HF10, an attenuated herpes simplex virus (HSV) type 1 clone, lacks neuroinvasiveness and protects mice against lethal challenge with HSV types 1 and 2.
Herpes simplex virus (HSV), a neurotropic virus, establishes life-long and, although rare, life-threatening infection in humans, and it may precipitate substantial medical and psychosocial morbidity. Here we show that HSV-1 strain HF clone 10 (HF10) exhibits impaired neuroinvasiveness in peripheral olfactory, vomeronasal and trigeminal conduits following intranasal as well as corneal inoculation. HF10 attenuation likely arises from multiple defects of HSV genes, so that HF10 will not revert to a virulent phenotype. Intranasal vaccination of mice with HF10 conferred significant protection against lethal challenge with HSV-1 and HSV-2 via the intranasal and intravaginal routes. Thus, we propose that HF10 explicitly meets the prerequisites for a candidate live attenuated HSV vaccine.
Mori
Isamu
I
Department of Microbiology and Immunology, Aichi Medical University School of Medicine, 480-1195, Japan. isamor@aichi-med-u.ac.jp
Liu
Beixing
B
Goshima
Fumi
F
Ito
Hiroyasu
H
Koide
Naoki
N
Yoshida
Tomoaki
T
Yokochi
Takashi
T
Kimura
Yoshinobu
Y
Nishiyama
Yukihiro
Y
D000818:Animals; D001835:Body Weight; D004195:Disease Models, Animal; D005260:Female; D006561:Herpes Simplex / Q000473:pathology / Q000517:prevention & control*; D022301:Herpes Simplex Virus Vaccines / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000276:immunology* / Q000472:pathogenicity*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000821:virology*; D009832:Olfactory Nerve / Q000821:virology; D014276:Trigeminal Nerve / Q000821:virology; D014613:Vaccines, Attenuated / Q000276:immunology; D019147:Vomeronasal Organ / Q000821:virology
D022301:Herpes Simplex Virus Vaccines; D014613:Vaccines, Attenuated