[Effect of surface property of different polyether-ester copolymers on growth of smooth muscle cells and endothelial cells].
OBJECTIVE
To investigate the effect of surface property of different polyether-ester block copolymers [poly(ethylene glycol-terephthalate)/poly(butylene terephthalate), PEGT/PBT] on the growth of smooth muscle cells (SMCs) and endothelial cells(ECs).


METHODS
Three kinds of copolymers were synthesized, which were 1000-T20 (group A), 1000PEGT70/PBT30 (group B) and 600PEGT70/PBT30 (group C). The water-uptake and contact angle of three polyether-ester membranes were determined. The canine aorta smooth muscle cells and external jugular vein endothelial cells were primarily harvested, subcultured, and then identified. The proliferation of SMCs and ECs on the different polyether-ester membranes were investigated.


RESULTS
The water-uptake of three copolymers arranged as the sequence of group C<group A<group B, and contact angle as the sequence of group C>group A> group B, indicating group B being more hydrophilic. However, smooth muscle cells and endothelial cells grew poorly on the membrane of group B after low density seeding, but proliferated well on the membranes of group A and group C.


CONCLUSIONS
In contrast with more hydrophilic 1000PEGT70/PBT30, moderately hydrophilic 1000-T20 and 600PEGT70/PBT30 has better compatibility with vascular cells. The above results indicate that the vascular cells can grow well on moderately hydrophilic PEGT/PBT and that PEGT/PBT can be used in vascular tissue engineering.
Chen
Liang
L
Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P. R. China. yangcai3133@sina.com
Xi
Tingfei
T
Yang
Zhiming
Z
Wang
Wei
W
Liu
Qingyang
Q
Wu
Yingfeng
Y
Gu
Yongquan
Y
Wang
Jiancai
J
Feng
Zengguo
Z
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D002478:Cells, Cultured; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology; D004987:Ethers / Q000737:chemistry; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering
D001672:Biocompatible Materials; D004987:Ethers; D011091:Polyesters; D011108:Polymers