title: Characterization of a humanized monoclonal antibody recognizing clumping factor A expressed by Staphylococcus aureus.
abstract: We report the humanization and characterization of monoclonal antibody (MAb) T1-2 or tefibazumab, a monoclonal antibody that recognizes clumping factor A expressed on the surface of Staphylococcus aureus. We demonstrate that the binding kinetics of MAb T1-2 is indistinguishable compared to that of its murine parent. Furthermore, MAb T1-2 is shown to enhance the opsonophagocytic uptake of ClfA-coated latex beads, protect against an intravenous challenge in a prophylactic model of rabbit infective endocarditis, and enhance the efficacy of vancomycin therapy in a therapeutic model of established infective endocarditis.
authors.affiliation: Inhibitex, Inc., 9005 Westside Parkway, Suite 400, Alpharetta, GA 30004, USA.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Domanski
authors.forename: Pratiksha R
authors.initials: PR
authors.lastname: Patel
authors.forename: Arnold S
authors.initials: AS
authors.lastname: Bayer
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Hall
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Syribeys
authors.forename: Elena L
authors.initials: EL
authors.lastname: Gorovits
authors.forename: Dawn
authors.initials: D
authors.lastname: Bryant
authors.forename: John H
authors.initials: JH
authors.lastname: Vernachio
authors.forename: Jeff T
authors.initials: JT
authors.lastname: Hutchins
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Patti
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood / Q000276:immunology*; D002460:Cell Line; D003030:Coagulase / Q000276:immunology*; D006801:Humans; D011817:Rabbits; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D003030:Coagulase