Posttransplant hematopoiesis in patients undergoing sibling allogeneic stem cell transplantation reflects that of their respective donors although with a lower functional capability.
We tested the principle of whether patient long-term hematopoiesis following allogeneic stem cell transplantation (allo-SCT) reflects the characteristics of the hematopoiesis of their respective donor. For this purpose, we analyzed bone marrow (BM) hematopoiesis using long-term cultures (LTC), delta assays, and clonogeneic assays as well as CD34+ cells and their subsets by flow cytometry in a series of 37 patients undergoing allo-SCT, and we compared it to that of their respective human leukocyte antigen-matched sibling donors in a paired study performed more than 1 year after the transplant procedure. Interestingly, the main factor that influenced post-allo-SCT BM hematopoiesis in the long term was donor hematopoiesis. Nevertheless, compared to their respective donors, patients exhibited a significantly lower number of colony-forming units granulomonocytic, burst-forming units erythroid, and immature progenitors (CD34++/CD38dim/CD90+/CD133+ cells, LTC-initiating cells, and colonies generated in the delta assay). Moreover, BM stromal function was diminished in patients undergoing allo-SCT compared to their donors. In addition, the presence of chronic graft-versus-host disease under immunosuppressive treatment also conditioned an impaired hematopoietic function. In summary, our study shows that BM hematopoiesis evaluated more than 1 year after an allo-SCT mainly reproduces that of their respective donors, although with a significantly decreased in vitro activity.
Sanchez-Guijo
Fermin M
FM
Servicio de Hematologia, Hospital Universitario de Salamanca, Universidad de Salamanca, Spain.
Sanchez-Abarca
Luis-Ignacio
LI
Villaron
Eva
E
Lopez-Holgado
Natalia
N
Alberca
Mercedes
M
Vazquez
Lourdes
L
Perez-Simon
Jose A
JA
Lopez-Fidalgo
Jesus
J
Orfao
Alberto
A
Caballero
Maria-Dolores
MD
Del Cañizo
Maria-Consuelo
MC
San Miguel
Jesus F
JF
D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D015672:Erythroid Precursor Cells / Q000166:cytology; D005260:Female; D006086:Graft vs Host Disease / Q000503:physiopathology; D042381:Granulocyte Precursor Cells / Q000166:cytology; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D017154:Stromal Cells / Q000166:cytology; D018183:Transplantation Chimera; D014184:Transplantation, Homologous
D015703:Antigens, CD