Hypoxic-ischemic insult decreases glutamate uptake by hippocampal slices from neonatal rats: prevention by guanosine.
Brain injury secondary to hypoxic-ischemic disease is the predominant form of damage encountered in the perinatal period. The impact of neonatal hypoxia-ischemia (HI) in 7-day-old pups on the high-affinity [3H] glutamate uptake into hippocampal slices at different times after insult was examined. Immediately following, and 1 day after the insult there was no effect. But at 3 to 5 days after the HI insult, glutamate uptake into the hippocampus was markedly reduced; however, after 30 or 60 days the glutamate uptake into hippocampal slices returned to control levels. Also, this study demonstrated the effect of the nucleoside guanosine (Guo) on the [3H] glutamate uptake in neonatal HI injury, maintaining the [3H] glutamate uptake at control levels when injected before and after insult HI. We conclude that neonatal HI influences glutamate uptake a few days following insult, and that guanosine prevents this action.
Moretto
M B
MB
Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, RS, Brazil.
Arteni
N S
NS
Lavinsky
D
D
Netto
C A
CA
Rocha
J B T
JB
Souza
D O
DO
Wofchuk
S
S
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D020520:Brain Infarction / Q000209:etiology / Q000517:prevention & control*; D018698:Glutamic Acid / Q000378:metabolism*; D006151:Guanosine / Q000008:administration & dosage*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D020925:Hypoxia-Ischemia, Brain / Q000150:complications / Q000378:metabolism*; D066298:In Vitro Techniques; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D006151:Guanosine; D018698:Glutamic Acid