Investigation of Foscan interactions with plasma proteins.
The present study investigates the interaction of the second generation photosensitizer Foscan with plasma albumin and lipoproteins. Spectroscopic studies indicated the presence of monomeric and aggregated Foscan species upon addition to plasma protein solutions. Kinetics of Foscan disaggregation in albumin-enriched solutions were very sensitive to the protein concentration and incubation temperature. Kinetic analysis demonstrated that two types of Foscan aggregated species could be involved in disaggregation: dimers with a rate constant of k1 = (2.30+/-0.15) x 10(-3) s(-1) and higher aggregates with rate constants varying from (0.55+/-0.04) x 10(-3) s(-1) for the lowest to the (0.17+/-0.02) x 10(-3) s(-1) for the highest albumin concentration. Disaggregation considerably increased with the temperature rise from 15 degrees C to 37 degrees C. Compared to albumin, Foscan disaggregation kinetics in the presence of lipoproteins displayed poorer dependency on lipoprotein concentrations and smaller variations in disaggregation rate constants. Gel-filtration chromatography analysis of Foscan in albumin solutions demonstrated the presence of aggregated fraction of free, non-bound to protein Foscan and monomeric Foscan, bound to protein.
Sasnouski
Siarhei
S
Centre Alexis Vautrin, CRAN UMR 7039 CNRS-INPL-UHP, 54511 Vandoeuvre-Les-Nancy cedex, France.
Zorin
Vladimir
V
Khludeyev
Ivan
I
D'Hallewin
Marie-Ange
MA
Guillemin
François
F
Bezdetnaya
Lina
L
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D007700:Kinetics; D008027:Light; D008074:Lipoproteins / Q000097:blood*; D008652:Mesoporphyrins / Q000737:chemistry* / Q000378:metabolism; D017319:Photosensitizing Agents / Q000378:metabolism*; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000378:metabolism*; D012710:Serum Albumin, Bovine; D012996:Solutions; D013057:Spectrum Analysis
D008074:Lipoproteins; D008652:Mesoporphyrins; D017319:Photosensitizing Agents; D012709:Serum Albumin; D012996:Solutions; D012710:Serum Albumin, Bovine; C072269:temoporfin