title: Secretion of matrix metalloproteinase-9 by macrophages, in vitro, in response to Helicobacter pylori.
abstract: We have previously shown that matrix metalloproteinase-9 (MMP-9) activity is greatly enhanced within the active chronic inflammation of Helicobacter pylori infected individuals, of which a major fraction derives from macrophages in the tissue. Here, we have investigated the ability of macrophages to secrete MMPs in response to H. pylori. Human macrophages secrete MMP-9 in response to live and inactivated H. pylori, as well as to specific bacterial products. Protein kinase C, phosphatiolylinositol 3-kinase and calcium uptake channels all play a role in MMP-9 secretion, whereas neither tumour necrosis factor alpha, interleukin-8, nor interleukin-1beta autocrine stimulation appear to contribute. We conclude that human macrophages have the ability to react directly against several H. pylori derived factors, utilising several signalling pathways.
authors.affiliation: Departments of Medical Microbiology and Immunology, Göteborg University, Sweden. p.j.bergin@qmul.ac.uk
authors.forename: Philip James
authors.initials: PJ
authors.lastname: Bergin
authors.forename: Wen
authors.initials: W
authors.lastname: Sicheng
authors.forename: Pan-Hammarström
authors.initials: PH
authors.lastname: Qiang
authors.forename: Quiding-Järbrink
authors.initials: QJ
authors.lastname: Marianne
mesh_terms: D001483:Base Sequence; D016207:Cytokines / Q000378:metabolism; D015870:Gene Expression; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D066298:In Vitro Techniques; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D009500:Neutralization Tests; D012313:RNA / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D019714:Tissue Inhibitor of Metalloproteinases / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D019714:Tissue Inhibitor of Metalloproteinases; D012313:RNA; D020780:Matrix Metalloproteinase 9