title: miBLAST: scalable evaluation of a batch of nucleotide sequence queries with BLAST.
abstract: A common task in many modern bioinformatics applications is to match a set of nucleotide query sequences against a large sequence dataset. Existing tools, such as BLAST, are designed to evaluate a single query at a time and can be unacceptably slow when the number of sequences in the query set is large. In this paper, we present a new algorithm, called miBLAST, that evaluates such batch workloads efficiently. At the core, miBLAST employs a q-gram filtering and an index join for efficiently detecting similarity between the query sequences and database sequences. This set-oriented technique, which indexes both the query and the database sets, results in substantial performance improvements over existing methods. Our results show that miBLAST is significantly faster than BLAST in many cases. For example, miBLAST aligned 247 965 oligonucleotide sequences in the Affymetrix probe set against the Human UniGene in 1.26 days, compared with 27.27 days with BLAST (an improvement by a factor of 22). The relative performance of miBLAST increases for larger word sizes; however, it decreases for longer queries. miBLAST employs the familiar BLAST statistical model and output format, guaranteeing the same accuracy as BLAST and facilitating a seamless transition for existing BLAST users.
authors.affiliation: Department of Electrical Engineering and Computer Science, University of Michigan Ann Arbor, MI 48109, USA.
authors.forename: You Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Andrew
authors.initials: A
authors.lastname: Boyd
authors.forename: Brian D
authors.initials: BD
authors.lastname: Athey
authors.forename: Jignesh M
authors.initials: JM
authors.lastname: Patel
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D019295:Computational Biology; D030561:Databases, Nucleic Acid; D006801:Humans; D020407:Internet; D015233:Models, Statistical; D015345:Oligonucleotide Probes / Q000737:chemistry; D018401:Sample Size; D016415:Sequence Alignment; D012984:Software; D013997:Time Factors
chemical_list: D015345:Oligonucleotide Probes