The histone chaperone SET/TAF-Ibeta interacts functionally with the CREB-binding protein.
The oncoprotein SET/TAF-Ibeta is a histone chaperone which is involved in cell-cycle control and chromatin remodeling. Confocal laser scanning microscopy reveals that SET is localized in distinct foci of variable size throughout the nucleoplasm of interphase cells. We report here that SET interacts directly with the acetyltransferase CREB-binding protein (CBP) and enhances the transactivation potential of the transcription coactivator. Our data suggest that the histone chaperone SET regulates the CBP-mediated transcription and may indicate a general principle by which transcriptional regulators cooperate with histone chaperones for gene activation.
Karetsou
Zoe
Z
Laboratory of Biological Chemistry, Medical School, University of Ioannina, 451 10 Ioannina, Greece.
Martic
Goran
G
Sflomos
George
G
Papamarcaki
Thomais
T
D019921:Biotinylation; D050882:CREB-Binding Protein; D002453:Cell Cycle; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins; D019084:Fluorescent Antibody Technique, Indirect; D005982:Glutathione Transferase / Q000378:metabolism; D006367:HeLa Cells; D056488:Histone Chaperones; D006657:Histones / Q000737:chemistry*; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D018832:Molecular Chaperones / Q000737:chemistry; D009154:Mutation; D009687:Nuclear Proteins / Q000737:chemistry*; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry; D015534:Trans-Activators / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D006657:Histones; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D011994:Recombinant Proteins; C513473:SET protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; D005982:Glutathione Transferase