title: Diverse roles of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in the growth of glioblastoma cells in vivo.
abstract: Hepatocyte growth factor activator inhibitor type-1 (HAI-1) is an integral-membrane proteinase inhibitor. In this study, we examined the effects of HAI-1 on human glioblastoma cells. Two glioblastoma cell lines (YKG-1, U251) were stably transfected with expression plasmid harboring mature membrane-form or truncated secreted-form HAI-1. Culture characteristics were not altered by the expression of HAI-1, whereas in vitro invasiveness of U251 was suppressed. On the other hand, the expression of membrane-form HAI-1 resulted in significantly enhanced tumorigenicity of both cell lines in vivo. In contrast, secreted-form HAI-1 did not promote the tumorigenicity. These results suggest that HAI-1 may play complex roles in progression of glioblastoma cells, and membrane-form HAI-1 may mediate an undefined important signaling in the cells.
authors.affiliation: Second Department of Pathology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Miyata
authors.forename: Shunro
authors.initials: S
authors.lastname: Uchinokura
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Fukushima
authors.forename: Ryouichi
authors.initials: R
authors.lastname: Hamasuna
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Itoh
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Akiyama
authors.forename: Shinichi
authors.initials: S
authors.lastname: Nakano
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Wakisaka
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kataoka
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D053491:Proteinase Inhibitory Proteins, Secretory
chemical_list: D008562:Membrane Glycoproteins; D053491:Proteinase Inhibitory Proteins, Secretory; C109149:SPINT1 protein, human; C504591:Spint1 protein, mouse