title: Effect of arachidonic, eicosapentaenoic and docosahexaenoic acids on the oxidative status of C6 glioma cells.
abstract: n-3 polyunsaturated fatty acids (PUFAs) have been described to have beneficial effects on brain development and in the prevention and treatment of brain damage. C6 glioma cells were incubated with 100 microM of either C20:4n-6 (ARA), or C20:5n-3 (EPA), or C22:6n-3 (DHA) for different time periods to assess whether these acids altered the cellular oxidative state. The ARA and EPA were promptly metabolised to C22:4n-6 and C22:5n-3, respectively, whereas DHA treatment simply increased the amount of DHA in the cells. Cell viability was not affected by ARA, while a cytotoxic effect was observed 72 h after n-3 PUFAs supplementation. The levels of reactive oxygen species and thiobarbituric acid-reactive substances were significantly higher in DHA-treated cells than in EPA- and ARA-treated groups. This modification in the oxidative cellular status was also highlighted by a significant increase in catalase activity and a decrease in glutathione content in DHA-supplemented cells. Glucose-6-phosphate dehydrogenase activity, an enzyme involved in redox regulation, and O2*- release were significantly increased both in EPA and DHA groups. The effect of DHA was more severe than that of EPA. No significant changes were observed in the ARA group with respect to untreated cells. These data show that EPA and DHA induce alterations in the oxidative status that could affect the glial function.
authors.affiliation: Food Science, Nutrition and Health Department, Intituto Superiore di Sanità, Rome, Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Leonardi
authors.forename: Lucilla
authors.initials: L
authors.lastname: Attorri
authors.forename: Rita
authors.initials: R
authors.lastname: Di Benedetto
authors.forename: Antonella
authors.initials: A
authors.lastname: Di Biase
authors.forename: Massimo
authors.initials: M
authors.lastname: Sanchez
authors.forename: Mirella
authors.initials: M
authors.lastname: Nardini
authors.forename: Serafina
authors.initials: S
authors.lastname: Salvati
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004281:Docosahexaenoic Acids / Q000494:pharmacology* / Q000633:toxicity; D015118:Eicosapentaenoic Acid; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology* / Q000633:toxicity; D005434:Flow Cytometry; D005909:Glioblastoma; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D013482:Superoxide Dismutase; D013858:Thiobarbiturates / Q000378:metabolism
chemical_list: D005231:Fatty Acids, Unsaturated; D013858:Thiobarbiturates; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D005954:Glucosephosphate Dehydrogenase; D013482:Superoxide Dismutase; D005978:Glutathione; C029684:thiobarbituric acid