[Establishment of a drug resistant leukemia cell line caused by mdr1 gene].
OBJECTIVE
To establish a drug resistant leukemia cell line caused by mdr1 gene for the mdr1 reversing research.


METHODS
The leukemia sensitive cell line K562 was transfected with mdr1 cDNA by liposome-induced gene transfection and selected by G418. The mdr1 phenotype was identified by RT-PCR, the P-gp expression was detected by flow cytometry, the function of P-gp was measured by Rh123 efflux experiment, and the sensitivity of cell lines to the drug was detected by MTT test. Results The transfected K562/mdr1 with mdr1 cDNA highly expressed mdr1 and showed a remarkable drug resistant feature.


CONCLUSIONS
mdr1 plays an important role in drug resistance and the mdr1 cell line established by gene transfection can provide a better cell model for mdr1 reversing research.
Gu
Ling
L
Department of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
Liu
Ting
T
Gong
Yu-ping
YP
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis* / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D019450:Genes, MDR / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D034622:RNA Interference; D014162:Transfection
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018076:DNA, Complementary