title: Biotransformation of bile acids by pathogenic actinomycetes Nocardia otitidiscaviarum and Amycolatopsis sp. strains.
abstract: Three sterol-type compounds (compounds 4, 5 and 6) were isolated from culture broth of pathogenic Nocardia otitidiscaviarum IFM 0988 and Amycolatopsis sp. IFM 0703 strains which were isolated from Japanese patients. The structures of the compounds were determined by NMR and mass spectrometric analyses. The structural studies indicated that compound 4 is a biotransformation product from cholic acid derivative in a nutrient culture medium constituent by a reductase-type enzyme, and the remaining two compounds 5 and 6 are also biotransformation ones by oxidase-type enzymes.
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicosis, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba-city, Chiba 260-8673, Japan. mikami@faculty.chiba-u.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Mukai
authors.forename: Katsukiyo
authors.initials: K
authors.lastname: Yazawa
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Mikami
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Harada
authors.forename: Udo
authors.initials: U
authors.lastname: Gräfe
mesh_terms: D039903:Actinobacteria / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism*; D001711:Biotransformation; D019826:Cholic Acid / Q000378:metabolism; D002853:Chromatography, Liquid; D003470:Culture Media; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009615:Nocardia / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism
chemical_list: D001647:Bile Acids and Salts; D003470:Culture Media; D010088:Oxidoreductases; D019826:Cholic Acid