Electrophoresis karyotype and chromosome-length polymorphism of Histoplasma capsulatum clinical isolates from Latin America.
Intact chromosomes of 19 clinical isolates of Histoplasma capsulatum recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed-field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5-7 bands, ranging from 1.3 to 10 Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7 Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of H. capsulatum varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source.
Canteros
Cristina Elena
CE
Departamento Micología, INEI ANLIS Dr. Carlos G. Malbrán, Buenos Aires, Argentina. ccanteros@anlis.gov.ar
Zuiani
María Fernanda
MF
Ritacco
Viviana
V
Perrotta
Diego E
DE
Reyes-Montes
María Rocío
MR
Granados
Julio
J
Zúñiga
Gerardo
G
Taylor
Maria Lucia
ML
Davel
Graciela
G
D001118:Argentina; D002871:Chromosome Banding; D015825:Chromosomes, Fungal / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006166:Guatemala; D006658:Histoplasma / Q000235:genetics / Q000302:isolation & purification; D006660:Histoplasmosis / Q000382:microbiology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007621:Karyotyping / Q000379:methods*; D007843:Latin America; D008800:Mexico; D011110:Polymorphism, Genetic