Local delivery of osteopontin attenuates vascular remodeling by altering matrix metalloproteinase-2 in a rabbit model of aortic injury.
OBJECTIVE
Vascular remodeling, often accelerated after cardiovascular procedures, may result in stenosis or aneurysm formation. The bone-associated protein osteopontin has been suggested to be involved in vascular remodeling, yet the effect of locally applied osteopontin in an acute vascular injury model of aortic calcification has not been examined.


METHODS
Vascular healing of rabbit thoracic aortas treated locally with recombinant osteopontin (dose: 1 microg; n = 16) or albumin (control, n = 16) after acute injury created by end-to-end anastomosis was evaluated. Matrix metalloproteinase-2 level was quantified by gelatin zymography. Proliferation of smooth muscle cells was detected by immunostaining for proliferative cell nuclear antigen.


RESULTS
Osteopontin-treated aortas showed significantly diminished vascular remodeling with less calcification (P = .001) and reduced anastomotic luminal stenosis (4% vs 28%, P = .002) compared with controls 2 months postsurgery. Moreover, osteopontin-treated aortas revealed a thickened adventitia with increased fibrosis (P = .006). Matrix metalloproteinase-2 level was up-regulated 2-fold with osteopontin treatment compared with control at 1 week, returning to baseline by 1 month. Staining for proliferation cell nuclear antigen disclosed an increase in proliferation cell nuclear antigen-positive smooth muscle cells in the media of osteopontin-treated aortas at 1 week, normalizing by 1 month.


CONCLUSIONS
These data suggest a beneficial effect of locally applied osteopontin after acute injury possibly by altering matrix metalloproteinase-2 activity and smooth muscle cell proliferation. Brief application of osteopontin may effectively enhance vascular healing by reducing calcification and thus maintaining luminal integrity. The role of the osteopontin-related increase in adventitial fibrosis on vascular healing has to be explored.
Seipelt
Ralf G
RG
Department of Surgey, Children's Memorial Hospital, Northwestern Univeristy Feinberg School of Medicine, Chicago, IL 60614, USA.
Backer
Carl L
CL
Mavroudis
Constantine
C
Stellmach
Veronica
V
Cornwell
Mona
M
Seipelt
Ingrid M
IM
Schoendube
Friedrich A
FA
Crawford
Susan E
SE
D000818:Animals; D001013:Aorta, Thoracic / Q000473:pathology*; D002114:Calcinosis; D002317:Cardiovascular Agents / Q000494:pharmacology*; D003251:Constriction, Pathologic / Q000517:prevention & control; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D023421:Models, Animal; D053495:Osteopontin; D011817:Rabbits; D012795:Sialoglycoproteins / Q000494:pharmacology*; D014652:Vascular Diseases / Q000517:prevention & control; D014945:Wound Healing / Q000187:drug effects*
D002317:Cardiovascular Agents; D012795:Sialoglycoproteins; D053495:Osteopontin; D020778:Matrix Metalloproteinase 2