title: Inducement and reversal of tetracycline resistance in Escherichia coli K-12 and expression of proton gradient-dependent multidrug efflux pump genes.
abstract: Expression of eight transporter genes of Escherichia coli K-12 and its DeltaacrAB mutant prior to and after induction of both strains to tetracycline resistance and after reversal of induced resistance were analyzed by quantitative reverse transcriptase PCR. All transporter genes were overexpressed after induced resistance with acrF being 80-fold more expressed in the DeltaacrAB tetracycline-induced strain.
authors.affiliation: Unit of Mycobacteriology, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua Junqueira 96, 1349-008 Lisbon, Portugal.
authors.forename: Miguel
authors.initials: M
authors.lastname: Viveiros
authors.forename: Ana
authors.initials: A
authors.lastname: Jesus
authors.forename: Mafalda
authors.initials: M
authors.lastname: Brito
authors.forename: Clara
authors.initials: C
authors.lastname: Leandro
authors.forename: Marta
authors.initials: M
authors.lastname: Martins
authors.forename: Diane
authors.initials: D
authors.lastname: Ordway
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Molnar
authors.forename: Joseph
authors.initials: J
authors.lastname: Molnar
authors.forename: Leonard
authors.initials: L
authors.lastname: Amaral
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D048168:Escherichia coli K12 / Q000187:drug effects*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008826:Microbial Sensitivity Tests; D009154:Mutation; D017494:Proton Pumps / Q000378:metabolism*; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance
chemical_list: D000900:Anti-Bacterial Agents; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D017494:Proton Pumps; D013752:Tetracycline