[Cloning and sequencing of heat shock protein 60 (HSP60) gene of Legionella pneumophila].
OBJECTIVE
To obtain the heat shock protein 60 (HSP60) gene of Legionella pneumophila and clone it into plasmid pUC18 for nucleotide sequencing.


METHODS
The HSP60 gene of Legionella pneumophila was amplified by PCR, then inserted into pUC18 vector and transformed into Escherichia coli (E. coli) strain JM109. The recombinant plasmids were identified by restriction analysis and PCR. HSP60 gene was sequenced by the dideoxy chain termination method, then analyzed and compared with that reported in GenBank.


RESULTS
The HSP60 gene of 1647 bp in length was successfully cloned. Comparison of the sequence of HSP60 gene with that reported in GenBank showed that the identities were 98%.


CONCLUSIONS
A confirmed HSP60 gene has been obtained in this paper. The study laid a good foundation of the recombination,expression and research of the gene.
Liao
Tao
T
Department of Parasitology and Morphology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Chen
Jian-ping
JP
Liu
Ming-jie
MJ
D001483:Base Sequence; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D016952:Legionella pneumophila / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D019444:Vaccines, DNA
D018834:Chaperonin 60; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D019444:Vaccines, DNA