title: Cell lysis directed by sigmaE in early stationary phase and effect of induction of the rpoE gene on global gene expression in Escherichia coli.
abstract: It has been shown that Escherichia coli cells with increased expression of the rpoE gene encoding sigma(E) exhibit enhanced cell lysis in early stationary phase. Further analysis of the lysis phenomenon was performed using a transient expression system of the rpoE gene and by DNA microarray. The former analysis revealed a sigma(E)-directed cell lysis, specific for early stationary phase but not for the exponential phase. The microarray analysis with RNAs from exponential and early stationary phase cells revealed that a large number of genes were up- or down-regulated when the rpoE gene was induced, and that several genes were induced in a phase-specific manner. The upregulated genes include many previously identified sigma(E) regulon genes, suggesting that a large number of genes are under the control of sigma(E) in this organism. These genes are involved in various cellular activities, including the cell envelope, cellular processes, regulatory functions, transport and translation. Genes that are presumably related to phase-specific cell lysis in E. coli are discussed.
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Md Shahinur
authors.initials: MS
authors.lastname: Kabir
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamashita
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Koyama
authors.affiliation: Graduate School of Information Science, Nara Institute of Science and Technology, Nara 630-01, Japan.
authors.forename: Taku
authors.initials: T
authors.lastname: Oshima
authors.affiliation: Graduate School of Information Science, Nara Institute of Science and Technology, Nara 630-01, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Kurokawa
authors.affiliation: R&E Center of Informatics, Nara Institute of Science and Technology, Nara 630-01, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Maeda
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Ryouichi
authors.initials: R
authors.lastname: Tsunedomi
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Murata
authors.affiliation: Laboratory of Plasma Membrane and Nuclear Signalling, Graduate School of Biostudies, 1302, Kyoto University, Kyoto 606-8502, Japan.|CREST, Nara Institute of Science and Technology, Nara 630-01, Japan.
authors.forename: Chieko
authors.initials: C
authors.lastname: Wada
authors.affiliation: R&E Center of Informatics, Nara Institute of Science and Technology, Nara 630-01, Japan.|CREST, Nara Institute of Science and Technology, Nara 630-01, Japan.
authors.forename: Hirotada
authors.initials: H
authors.lastname: Mori
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Yamada
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D012808:Sigma Factor / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D029968:Escherichia coli Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C063202:sporulation-specific sigma factors