Promoter analysis of Mucor rouxii delta9-desaturase: its implication for transcriptional regulation in Saccharomyces cerevisiae.
Promoter study was performed to understand the transcriptional control of delta9-desaturase gene of Mucor rouxii. Several putative cis-elements involved in lipid metabolism were mapped by computational analysis. 5' deletion analysis shows the presence of elements with repressing activity, especially in 122 bp located upstream of the transcription start site. Truncation of these repressor domains showed that the promoter of M. rouxii is functional in Saccharomyces cerevisiae without additional components and is insensitive to nutritional depletion. The promoter also drove effectively the expression of a M. rouxii delta12-desaturase gene, and the linoleic acid content increased with the age of the yeast culture in parallel with the promoter activity. This approach provides a genetic tool for programming heterologous protein production in the yeast.
Laoteng
Kobkul
K
Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), King Mongkut's University of Technology Thonburi, Bangkhuntien, Bangkok 10150, Thailand. kobkul@biotec.or.th
Cheevadhanarak
Supapon
S
Tanticharoen
Morakot
M
Maresca
Bruno
B
D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D005227:Fatty Acids / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D005821:Genetic Techniques; D005822:Genetic Vectors; D007763:Lac Operon; D050356:Lipid Metabolism; D008969:Molecular Sequence Data; D009089:Mucor / Q000201:enzymology*; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013230:Stearoyl-CoA Desaturase / Q000235:genetics*; D014158:Transcription, Genetic
D017931:DNA Primers; D005227:Fatty Acids; D013230:Stearoyl-CoA Desaturase