title: Exploiting pH mismatch in preparative high-performance liquid chromatographic recovery of ertapenem from mother liquor streams.
abstract: Preparative chromatography was successfully employed to recover ertapenem from mother liquor streams. The recovery process involved concentration of mother liquor stream by evaporation, purification by reversed-phase preparative high-performance liquid chromatography (HPLC), and removal of chromatographic solvents in the recovered fractions by evaporation. HPLC feed was prepared by stripping off the organic solvents from the mother liquor using a wiped-film evaporator. Purification was first carried out on a 25 cm x 0.46 cm analytical column packed with 10-microm Kromasil C8 particles and then scaled up to a 25 cm x 5 cm preparative column. Gram-level recovery of ertapenem with high purity was achieved by exploiting a novel approach based on pH mismatch between the feed and the eluent. Purified ertapenem streams from preparative HPLC runs were combined, evaporated and recycled into the crystallizer for ertapenem isolation.
authors.affiliation: Analytical Research, Merck Research Laboratories, Merck & Co., Inc., RY818-C220, P.O. Box 2000, Rahway, NJ 07065-0900, USA. anant_vailaya@merck.com
authors.forename: Anant
authors.initials: A
authors.lastname: Vailaya
authors.forename: Peter
authors.initials: P
authors.lastname: Sajonz
authors.forename: Osama
authors.initials: O
authors.lastname: Sudah
authors.forename: Vincent
authors.initials: V
authors.lastname: Capodanno
authors.forename: Roy
authors.initials: R
authors.lastname: Helmy
authors.forename: Firoz D
authors.initials: FD
authors.lastname: Antia
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D000077727:Ertapenem; D006863:Hydrogen-Ion Concentration; D007769:Lactams / Q000302:isolation & purification*; D017711:Nonlinear Dynamics; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D007769:Lactams; D047090:beta-Lactams; D000077727:Ertapenem