title: Manual solid-phase synthesis of glutathione analogs: a laboratory-based short course.
abstract: This chapter provides a manual for a laboratory-based short course to introduce the common techniques of solid-phase peptide synthesis (SPPS). The course provides students the opportunity to design and manually synthesize analogs of glutathione using relatively simple equipment available in any unsophisticated laboratory. The manual provides compact protocols for both the different steps of SPPS and the final cleavage of peptides from resin supports. We also introduce a simple method for the synthesis of combinatorial libraries of glutathione analogs that is suitable for those relatively unfamiliar with the field of peptide chemistry.
authors.affiliation: Department of Biochemistry, Tartu University, Estonia.
authors.forename: Ursel
authors.initials: U
authors.lastname: Soomets
authors.forename: Mihkel
authors.initials: M
authors.lastname: Zilmer
authors.forename: Ulo
authors.initials: U
authors.lastname: Langel
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D020650:Combinatorial Chemistry Techniques / Q000193:education / Q000295:instrumentation / Q000379:methods; D005978:Glutathione / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D007202:Indicators and Reagents; D007753:Laboratories; D008967:Molecular Biology / Q000193:education* / Q000295:instrumentation / Q000379:methods; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry; D019151:Peptide Library; D012117:Resins, Synthetic
chemical_list: D000596:Amino Acids; D007202:Indicators and Reagents; D009842:Oligopeptides; D019151:Peptide Library; D012117:Resins, Synthetic; D005978:Glutathione