title: Structural elucidation of A-74528, an inhibitor for 2',5'-phosphodiesterase isolated from Streptomyces sp.
abstract: A-74528 (1) is a metabolite of Streptomyces sp. discovered in the screening for 2',5'-oligoadenylate phosphodiesterase inhibitors. The planar structure of 1 was mainly elucidated by NMR techniques including natural abundance INADEQUATE, and the relative configuration and the conformation were elucidated by the analyses of NOEs and assessment of dihedral angles predicted by QUANTA/CHARMm computations and coupling constants. It was proved that 1 is a highly fused polyketide with a side-chain branching site that never appeared before from the nature.
authors.affiliation: Sankyo R&D Facility, Sankyo Co. Ltd., 2-58, Hiromachi 1-chome, Shinagawa-ku, Tokyo 140-8710, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Fujita
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kasuya
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Matsushita
authors.forename: Miwa
authors.initials: M
authors.lastname: Suga
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kizuka
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Iijima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ogita
mesh_terms: D004791:Enzyme Inhibitors / Q000737:chemistry*; D005095:Exoribonucleases / Q000037:antagonists & inhibitors*; D018942:Macrolides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D011083:Polycyclic Compounds / Q000737:chemistry*; D011753:Pyrones / Q000737:chemistry*; D013237:Stereoisomerism; D013302:Streptomyces / Q000201:enzymology*; D013329:Structure-Activity Relationship
chemical_list: C506219:A-74528; D004791:Enzyme Inhibitors; D018942:Macrolides; D011083:Polycyclic Compounds; D011753:Pyrones; D005095:Exoribonucleases; C029364:2'-phosphodiesterase