Interleukin-10 polymorphisms in Spanish patients with IBD.
BACKGROUND
Inflammatory bowel disease (IBD) is considered a heterogeneous, complex polygenic disease where both genetic and environmental factors are involved in the development of the disease. Interleukin-10 (IL-10) is a regulatory cytokine that might play an important role in disease pathogenesis. IL-10 contains single nucleotide polymorphisms and 2 polymorphic microsatellites in the 5'-flanking region. Our aim was to ascertain if any of these polymorphic markers is associated with IBD among Spanish patients.


METHODS
We genotyped 470 patients with IBD, 242 with ulcerative colitis and 228 with Crohn's disease (CD), and 572 ethnically matched controls for microsatellites IL-10R and IL-10G and 2 single nucleotide polymorphisms at positions -1082 and -819 in the proximal promoter of the gene.


RESULTS
IL-10G14 microsatellite allele as well as -1082G allele were significantly increased in patients with CD. The combined presence of both alleles in 1 individual notably increased the risk to develop CD (P = 0.00006, odds ratio = 3.18).


CONCLUSIONS
IL-10 polymorphisms contribute to susceptibility to CD in Spanish population.
Fernandez
Laura
L
Department of Clinical Immunology, Hospital Clinico San Carlos, Madrid, Spain. laura_ffranco@vodafone.es
Martinez
Alfonso
A
Mendoza
Juan L
JL
Urcelay
Elena
E
Fernandez-Arquero
Miguel
M
Garcia-Paredes
Julio
J
Diaz-Rubio
Manuel
M
de la Concha
Emilio G
EG
D001483:Base Sequence; D016022:Case-Control Studies; D015331:Cohort Studies; D003093:Colitis, Ulcerative / Q000453:epidemiology / Q000235:genetics*; D003424:Crohn Disease / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D015994:Incidence; D015212:Inflammatory Bowel Diseases / Q000453:epidemiology / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011336:Probability; D012016:Reference Values; D012720:Severity of Illness Index; D013030:Spain / Q000453:epidemiology
D016753:Interleukin-10