title: A colorimetric titration method for quantification of millimolar glucose in a pH 7.4 aqueous phosphate buffer.
abstract: 3-Pyridinylboronic acid is identified as a key sensing element for reversible sugar complexation in an aqueous solution at the physiological pH. The utility of a sensing element has been demonstrated through a simple colorimetric titration of glucose using absorption spectroscopy in the visible region. The mechanism of the high diol/triol binding affinity of pyridine boronic acid in the neutral pH is discussed based on the 1H and 11B NMR spectroscopic studies.
authors.affiliation: Department of Chemistry, The University of Akron, Akron, OH 44325-3601, USA.
authors.forename: Serhan
authors.initials: S
authors.lastname: Boduroglu
authors.forename: Jouliana M
authors.initials: JM
authors.lastname: El Khoury
authors.forename: D
authors.initials: D
authors.lastname: Venkat Reddy
authors.forename: Peter L
authors.initials: PL
authors.lastname: Rinaldi
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
mesh_terms: D001897:Boronic Acids; D002021:Buffers; D002138:Calibration; D003124:Colorimetry / Q000379:methods*; D005947:Glucose / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013057:Spectrum Analysis; D017186:Titrimetry
chemical_list: D001897:Boronic Acids; D002021:Buffers; D005947:Glucose