Presence of CRF09_cpx and complex CRF02_AG/CRF09_cpx recombinant HIV type 1 strains in Côte d'Ivoire, West Africa.
Based on partial env and pol (protease and RT) subtyping, we recently documented that the majority (>80%) of the HIV-1 strains that circulate in Côte d'Ivoire were CRF02_AG and about 11% were recombinants or could not be clearly assigned to a known subtype or CRF. In order to determine in more detail the precise structure of these viruses we sequenced the full-length genomes for six such strains. Bootscan and phylogenetic tree analysis showed that four strains were complex and unique CRF02_AG/CRF09_cpx recombinants, one was a CRF02_AG/CRF06_cpx recombinant, and one was a pure CRF09_cpx. Reanalysis of the remaining recombinants asserted the predominance of CRF09_cpx within intersubtype recombinants and circulation of CRF09_cpx in Côte d'Ivoire. More detailed analysis of the CRF09_cpx strains revealed also that part of the pol gene belonged to subtype K. This is the first time that such recombinants are described.
Toni
Thomas
T
PAC-CI, Abidjan, Côte d'Ivoire.
Adjé-Touré
Christiane
C
Vidal
Nicole
N
Minga
Albert
A
Huet
Charlotte
C
Borger
Marie-Yolande
MY
Recordon-Pinson
Patricia
P
Masquelier
Bernard
B
Nolan
Monica
M
Nkengasong
John
J
Fleury
Hervé J
HJ
Delaporte
Eric
E
Peeters
Martine
M
D007560:Cote d'Ivoire; D015686:Gene Products, env / Q000235:genetics; D015684:Gene Products, pol / Q000235:genetics; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D013045:Species Specificity
D015686:Gene Products, env; D015684:Gene Products, pol