Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals.
HIV-1 can be isolated from peripheral blood mononuclear cells and is easily propagated on primary cells in vitro. Here we describe the method for bulk isolation of the HIV-1 quasispecies and a limiting dilution virus isolation protocol by which single coexisting clones can be obtained. In addition, methods for propagation and titration of HIV-1 are provided.
Schuitemaker
Hanneke
H
Department of Clinical Vitro-Immunology, Sanquin Research at CLB, Academical Medical Center, Amsterdam, The Netherlands.
Kootstra
Neeltje A
NA
D015658:HIV Infections / Q000097:blood* / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification* / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D009500:Neutralization Tests / Q000379:methods; D010948:Viral Plaque Assay / Q000379:methods; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication / Q000502:physiology*