title: Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro.
abstract: We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS-2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (approximately 07:00 to 23:00 h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time-PCR analysis. DEX stimulation of human BEAS-2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes.
authors.affiliation: Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, Japan. burioka@grape.med.tottori-u.ac.jp
authors.forename: Naoto
authors.initials: N
authors.lastname: Burioka
authors.forename: Miyako
authors.initials: M
authors.lastname: Takata
authors.forename: Yoko
authors.initials: Y
authors.lastname: Okano
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Ohdo
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Fukuoka
authors.forename: Masanori
authors.initials: M
authors.lastname: Miyata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takane
authors.forename: Masahiro
authors.initials: M
authors.lastname: Endo
authors.forename: Hisashi
authors.initials: H
authors.lastname: Suyama
authors.forename: Eiji
authors.initials: E
authors.lastname: Shimizu
mesh_terms: D000328:Adult; D001980:Bronchi / Q000033:anatomy & histology; D018797:Cell Cycle Proteins; D002460:Cell Line; D002940:Circadian Rhythm / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D056950:Period Circadian Proteins; D020545:Respiratory Mucosa / Q000187:drug effects / Q000502:physiology
chemical_list: D018797:Cell Cycle Proteins; D005938:Glucocorticoids; D009687:Nuclear Proteins; C539022:PER1 protein, human; D056950:Period Circadian Proteins; D003907:Dexamethasone