Tracking movement in cell biology.
This article is an overview of techniques for measuring movement of proteins, vesicles and cells using digital image processing. Diverse techniques have been developed during the last decade. Both strong and weak points of each technique are discussed, together with short description on the actual application. Among these techniques, the optical flow estimation technique is explained in more detail and an example of protein movement analysis is described.
Miura
Kota
K
Cell Biology and Biophysics Programme, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany. miura@embl.de
D000465:Algorithms; D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods*; D009038:Motion; D010363:Pattern Recognition, Automated / Q000379:methods*; D021381:Protein Transport / Q000502:physiology*; D012815:Signal Processing, Computer-Assisted; D013382:Subtraction Technique