[Effect of expression of p53 in squamous cell carcinoma of larynx and mucosa adjacent to tumor on the biological behavior].
OBJECTIVE
To investigate the expression of p53 in squamous cell carcinoma of larynx (LSCC) and mucosa adjacent to LSCC and study the correlation between it and the biological behavior with retrospective analysis.


METHODS
p53 protein overexpression was detected in 76 squamous cell carcinoma and the tissue beside the LSCC with SP method of Immuno-histochemical staining [IHC].


RESULTS
p53 overexpression was observed in 47.4% of LSCC, 40.0% of carcinoma in situ, 22.2% of atypical hyperplasia (AtH), 0% of normal mucosa. p53 overexpression in parenchyma of LSCC as well as in the corresponding carcinoma in situ is unaminous completely. There was a significant difference between p53 positive staining in AtH and in carcinoma of larynx as well as carcinoma in situ, but p53 overexpression in AtH adjacent to the laryngeal carcinoma is apparently correlated with that in the corresponding parenchyma of carcinoma of larynx (P < 0.01). No correlation was found between p53 over expression and sex, age, TNM stage, location and T stage, but there was significant correlation between p53 over expression and differentiation grading (G1-->G2 + G3). It was noted that of the rate p53 overexpression was 68.8% in lymphatic metastasis group, which was higher than the percentage (41.7%) found in lymphatic nonmetastasis group (P > 0.05).


CONCLUSIONS
p53 overexpression are present in LSCC of different stages and AtH with significant correlation. p53 may play an important role in an early stage of malignant transformation of a subset of LSCC. Multiple-step carcinogenesis and malignancy as well as prognosis may be in association with p53-related tumorigenesis.
Luo
Keqiang
K
Department of Otolaryngology, China-Japan Friendship Hospital, Beijing, 100029, China. luokeqiang@vip.sina.com
Wang
Zhizhong
Z
Wang
Naya
N
Zhang
Xiaobo
X
Yang
Jisheng
J
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
D016159:Tumor Suppressor Protein p53