Comparison of the bacterial Enhancin-like proteins from Yersinia and Bacillus spp. with a baculovirus Enhancin.
Enhancins are a class of metalloproteases found in some baculoviruses that enhance viral infection by degrading the peritrophic membrane (PM) of the insect midgut. However, sequencing has revealed enhancin-like genes with 24-25% homology to viral enhancins, in the genomes of Yersinia pestis and Bacillus anthracis. AcMNPV does not encode enhancin therefore recombinant AcMNPV budded viruses (BVs) and polyhedra inclusion bodies (PIBs) were generated expressing the bacterial Enhancins. Bacterial Enhancins were found to be cytotoxic when compared to viral enhancin, however, larval bioassays suggested that the bacterial Enhancins did not enhance infection in the same way as viral Enhancin. This suggests that the bacterial Enhancins may have evolved a distinct biochemical function.
Galloway
C S
CS
School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading RG1 6AJ, UK.
Wang
P
P
Winstanley
D
D
Jones
I M
IM
D000818:Animals; D001407:Bacillus / Q000378:metabolism*; D016367:Baculoviridae / Q000378:metabolism*; D001681:Biological Assay; D016923:Cell Death; D002460:Cell Line; D007814:Larva; D045726:Metalloproteases / Q000378:metabolism / Q000494:pharmacology*; D018411:Spodoptera / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D015007:Yersinia / Q000378:metabolism*
D045726:Metalloproteases