title: Reticulon 3 is involved in membrane trafficking between the endoplasmic reticulum and Golgi.
abstract: Reticulons (RTNs) constitute a family of endoplasmic reticulum (ER)-associated proteins with a reticular distribution. Despite the implication of their neuronal isoforms in axonal regeneration, the function of their widely expressed isoforms is largely unknown. In this study, we examined the role of the ubiquitously expressed RTN3 in membrane trafficking. Ectopically expressed RTN3 exhibited heterogeneous patterns; filamentous, reticular, and granular distributions. The ER morphology changed accordingly. In cells where RTN3 displayed a filamentous/reticular distribution, protein transport between the ER and Golgi was blocked, and Golgi proteins were dispersed. In contrast, ERGIC-53, a marker for the ER-Golgi intermediate compartment, accumulated at the perinuclear region, and remained there even after cells were treated with agents that induce redistribution of Golgi proteins to the ER, indicating an inhibition of Golgi-to-ER transport of ERGIC-53. These results suggest that RTN3 plays a role in membrane trafficking in the early secretory pathway.
authors.affiliation: School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Wakana
authors.forename: Satoshi
authors.initials: S
authors.lastname: Koyama
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Nakajima
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Hatsuzawa
authors.forename: Masami
authors.initials: M
authors.lastname: Nagahama
authors.forename: Katsuko
authors.initials: K
authors.lastname: Tani
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Hauri
authors.forename: Paul
authors.initials: P
authors.lastname: Melançon
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Tagaya
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C084004:LMAN1 protein, human; D037241:Mannose-Binding Lectins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C476198:RTN3 protein, human; D011994:Recombinant Proteins