Porcine liver-expressed antimicrobial peptides, hepcidin and LEAP-2: cloning and induction by bacterial infection.
Hepcidin is a liver-expressed iron-regulating hormone that also is an antimicrobial peptide. Here we report the full-length cDNA sequences of porcine hepcidin and liver-expressed antimicrobial peptide-2 (LEAP-2). Porcine hepcidin and LEAP-2 cDNA sequences contain 411 and 525 bp, and encode predicted peptides of 82 and 77 amino acid residues, respectively. Both porcine hepcidin and LEAP-2 are highly expressed in liver and LEAP-2 also is expressed in intestinal tissues and kidney. Pigs infected with Salmonella enterica serovar Typhimurium showed inducible but differential expression of hepcidin and LEAP-2 in bone marrow and intestinal tissues. Conversely, although highly expressed in liver, expression of hepcidin mRNA in liver was not influenced by Salmonella infection. These findings provide fundamental comparative data showing the relationship of porcine hepcidin and LEAP-2 to other mammalian orthologs and indicate that bacterial infections influence their expression.
Sang
Yongming
Y
Department of Anatomy and Physiology, Kansas State University, Coles Hall 228, Manhattan, 66506-5802, USA.
Ramanathan
Balaji
B
Minton
J Ernest
JE
Ross
Chris R
CR
Blecha
Frank
F
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D064451:Hepcidins; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012480:Salmonella Infections / Q000235:genetics* / Q000382:microbiology; D019779:Salmonella enterica / Q000502:physiology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D034421:Sus scrofa / Q000235:genetics*
D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; C578702:HAMP protein, human; D064451:Hepcidins; D012333:RNA, Messenger