title: Changes in hepatic drug metabolizing enzymes and lipid peroxidation by methanol extract and major compound of Orostachys japonicus.
abstract: The effects of methanol extract and gallic acid (3,4,5-trihydroxybenzoic acid) of Orostachys japonicus A. Berger on hepatic drug metabolizing enzymes and lipid peroxidation were investigated in rats treated with bromobenzene. The methanol extract of Orostachys japonicus reduced the activities of phase I enzymes, aminopyrine N-demethylase and aniline hydroxylase, that had been increased by i.p. injection of bromobenzene. Gallic acid isolated from Orostachys japonicus also reduced the aniline hydroxylase activity, while it did not affect the aminopyrine N-demethylase activity. The methanol extract and gallic acid restored the activity of epoxide hydrolase which had been decreased by bromobenzene. Hepatic glutathione content was lowered, along with increase in hepatic lipid peroxide, by bromobenzene administration. The hepatic lipid peroxidation induced by bromobenzene was prevented with the methanol extract and gallic acid of Orostachys japonicus. However, the decrease in glutathione was not altered by gallic acid. The present results suggest that the methanol extract and gallic acid of Orostachys japonicus may protect liver from bromobenzene toxicity through, at least in part, inhibiting the cytochrome P450-dependent monooxygenase activities and enhancing the activity of epoxide hydrolase. Antioxidant effect also may contribute to the protection of Orostachys japonicus against the bromobenzene-induced hepatotoxicity.
authors.affiliation: Department of Oriental Medicine Resources, Research Institute of Korean Oriental Medicine, Sunchon National University, Suncheon, Jeonnam, Republic of Korea. flavonoid@empal.com
authors.forename: Jong Cheol
authors.initials: JC
authors.lastname: Park
authors.forename: Won Dong
authors.initials: WD
authors.lastname: Han
authors.forename: Jeong Ro
authors.initials: JR
authors.lastname: Park
authors.forename: Seong Hee
authors.initials: SH
authors.lastname: Choi
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Choi
mesh_terms: D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D001969:Bromobenzenes / Q000378:metabolism / Q000633:toxicity; D029627:Crassulaceae / Q000737:chemistry*; D005707:Gallic Acid / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001969:Bromobenzenes; D010936:Plant Extracts; D005707:Gallic Acid; C032036:bromobenzene; D000633:Aminopyrine N-Demethylase; D005978:Glutathione