title: Ultrastructure and histochemistry of rat myocardial capillary endothelial cells in response to diabetes and hypertension.
abstract: Insufficient growth and rarefaction of capillaries, followed by endothelial dysfunction may represent one of the most critical mechanisms involved in heart damage. In this study we examined histochemical and ultrastructural changes in myocardial capillary endothelium in two models of heart failure streptozotocin-induced diabetes mellitus (STZ) and NO-deficient hypertension in male Wistar rats. Diabetes was induced by a single i.v. dose of STZ (45 mg/kg) and chronic 9-week stage was analysed. To induce NO-deficient hypertension, animals were treated with inhibitor of NO synthase L-nitroarginine methylester (L-NAME) (40 mg/kg) for 4 weeks. Left ventricular tissue was processed for enzyme catalytic histochemistry of capillary alkaline phosphatase (AlPh), dipeptidyl peptidase IV (DPP IV), and endothelial NO synthase/NADPH-diaphorase (NOS) and for ultrastructural analysis. In diabetic and hypertensive rats, lower/absent AlPh and DPP IV activities were found in focal micro-areas. NOS activity was significantly reduced and persisted only locally. Quantitative evaluation demonstrated reduction of reaction product intensity of AlPh, DPP and NOS by 49.50%, 74.36%, 20.05% in diabetic and 62.93%, 82.71%, 37.65% in hypertensive rats. Subcellular alterations of endothelial cells were found in heart of both groups suggesting injury of capillary function as well as compensatory processes. Endothelial injury was more significant in diabetic animals, in contrast the adaptation was more evident in hypertensive ones. CONCLUDING: both STZ-induced diabetes- and NO-deficient hypertension-related cardiomyopathy were accompanied by similar features of structural remodelling of cardiac capillary network manifested as angiogenesis and angiopathy. The latter was however, predominant and may accelerate disappearance of capillary endothelium contributing to myocardial dysfunction.
authors.affiliation: Institute for Heart Research, Slovak Academy of Sciences, 840 05 Bratislava, Slovak Republic. usrdokru@savba.sk
authors.forename: Ludmila
authors.initials: L
authors.lastname: Okruhlicova
authors.forename: Narcis
authors.initials: N
authors.lastname: Tribulova
authors.forename: Peter
authors.initials: P
authors.lastname: Weismann
authors.forename: Ruzena
authors.initials: R
authors.lastname: Sotnikova
mesh_terms: D000818:Animals; D002196:Capillaries / Q000201:enzymology / Q000648:ultrastructure*; D048909:Diabetes Complications / Q000201:enzymology / Q000473:pathology; D003920:Diabetes Mellitus / Q000139:chemically induced / Q000473:pathology*; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000201:enzymology / Q000648:ultrastructure*; D006333:Heart Failure / Q000139:chemically induced / Q000209:etiology / Q000473:pathology*; D006973:Hypertension / Q000139:chemically induced / Q000150:complications / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000648:ultrastructure*; D019331:NG-Nitroarginine Methyl Ester; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
chemical_list: D013311:Streptozocin; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester