title: [Effects of anther--pretreatments on the survival percentage of isolated microspores of maize].
abstract: Using maize anthers of 2 cultivars as experimental materials, the effects of mannitol, colchcine and proline concentration in pretreatment solution and pretreating temperature on the survival percentage of isolated microspore were investigated. The survival percentage of isolated microspores were able to be respectively and obviously improved by pretreating anthers with mannitol from low to high concentration (0.35 mol/L, 24h --> 0.40 mol/L, 24h --> 0.45 mol/L, 24h), or with colchicine 200 mg/L or with proline 200 mg/L and pretreating at 15 degrees C for 48h. The probable mechanism of pretreatments was discussed.
authors.affiliation: Shanghai Key Lab. of Agricultural Genetics and Breeding, Agro-Biotech Research Center, Shanghai Academy of Agricultural Sciences, Shanghai 201106.
authors.forename: Rui Ju
authors.initials: RJ
authors.lastname: Lu
authors.forename: Yi Fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Yue Fang
authors.initials: YF
authors.lastname: Sun
authors.forename: Run Mei
authors.initials: RM
authors.lastname: Zhou
authors.forename: Jian Hua
authors.initials: JH
authors.lastname: Huang
mesh_terms: D003078:Colchicine / Q000494:pharmacology; D035264:Flowers / Q000187:drug effects* / Q000502:physiology*; D008353:Mannitol / Q000494:pharmacology; D011392:Proline / Q000494:pharmacology; D013696:Temperature; D003313:Zea mays / Q000187:drug effects* / Q000502:physiology*
chemical_list: D008353:Mannitol; D011392:Proline; D003078:Colchicine