A new small temperate DNA phage BcP15 isolated from Burkholderia cepacia DR11.
A Burkholderia cepacia DR11 strain was isolated during the survey of microorganisms from coastal water of deltaic Sunderbans. This strain always released temperate phage BcP15 into culture supernatant. UV irradiation of the strain also induced phage induction. The phage titer was 2.3 x 10(8). New temperate phage BcP15 has unusual structure. It has a hexagonal head, 65 nm in diameter and a tail 200 nm long, attached with single thick wavy tail fiber (424-705 nm). Phage DNA is double stranded 11.9 kb long. Southern hybridization result indicated that the phage DNA was in lysogenic state into the B. cepacia DR11 genome. SDS-PAGE of phage protein showed two major bands of molecular weight 20 kDa and 40 kDa.
Hens
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DK
National Institute of Cholera and Enteric Diseases, Kolkata, India.
Ghosh
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AN
Kumar
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D001435:Bacteriophages / Q000302:isolation & purification* / Q000648:ultrastructure*; D015139:Blotting, Southern; D016956:Burkholderia cepacia / Q000302:isolation & purification / Q000528:radiation effects / Q000821:virology*; D002876:Chromosomes, Bacterial / Q000821:virology; D004247:DNA; D004279:DNA, Viral / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008242:Lysogeny; D008854:Microscopy, Electron; D008970:Molecular Weight; D019251:Nucleocapsid / Q000648:ultrastructure; D014764:Viral Proteins / Q000032:analysis / Q000302:isolation & purification; D017376:Viral Tail Proteins / Q000648:ultrastructure; D014775:Virus Activation; D014871:Water Microbiology
D004279:DNA, Viral; D014764:Viral Proteins; D017376:Viral Tail Proteins; D004247:DNA