Secretion of matrix metalloproteinase-9 by macrophages, in vitro, in response to Helicobacter pylori.
We have previously shown that matrix metalloproteinase-9 (MMP-9) activity is greatly enhanced within the active chronic inflammation of Helicobacter pylori infected individuals, of which a major fraction derives from macrophages in the tissue. Here, we have investigated the ability of macrophages to secrete MMPs in response to H. pylori. Human macrophages secrete MMP-9 in response to live and inactivated H. pylori, as well as to specific bacterial products. Protein kinase C, phosphatiolylinositol 3-kinase and calcium uptake channels all play a role in MMP-9 secretion, whereas neither tumour necrosis factor alpha, interleukin-8, nor interleukin-1beta autocrine stimulation appear to contribute. We conclude that human macrophages have the ability to react directly against several H. pylori derived factors, utilising several signalling pathways.
Bergin
Philip James
PJ
Departments of Medical Microbiology and Immunology, Göteborg University, Sweden. p.j.bergin@qmul.ac.uk
Sicheng
Wen
W
Qiang
Pan-Hammarström
PH
Marianne
Quiding-Järbrink
QJ
D001483:Base Sequence; D016207:Cytokines / Q000378:metabolism; D015870:Gene Expression; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*; D006801:Humans; D066298:In Vitro Techniques; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D009500:Neutralization Tests; D012313:RNA / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D019714:Tissue Inhibitor of Metalloproteinases / Q000276:immunology / Q000378:metabolism
D016207:Cytokines; D019714:Tissue Inhibitor of Metalloproteinases; D012313:RNA; D020780:Matrix Metalloproteinase 9