Phenotypic characterization of chondrosarcoma-derived cell lines.
Gene expression profiling of three chondrosarcoma derived cell lines (AD, SM, 105KC) showed an increased proliferative activity and a reduced expression of chondrocytic-typical matrix products compared to primary chondrocytes. The incapability to maintain an adequate matrix synthesis as well as a notable proliferative activity at the same time is comparable to neoplastic chondrosarcoma cells in vivo which cease largely cartilage matrix formation as soon as their proliferative activity increases. Thus, the investigated cell lines are of limited value as substitute of primary chondrocytes but might have a much higher potential to investigate the behavior of neoplastic chondrocytes, i.e. chondrosarcoma biology.
Schörle
C M
CM
Osteoarticular and Arthritis Research, Department of Pathology, University of Erlangen-Nürnberg, Krankenhausstr. 8-10, D-91054 Erlangen, Germany.
Finger
F
F
Zien
A
A
Block
J A
JA
Gebhard
P M
PM
Aigner
T
T
D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D019902:Chondrocytes / Q000166:cytology* / Q000502:physiology*; D002813:Chondrosarcoma / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D016133:Polymerase Chain Reaction
D017931:DNA Primers