title: [Investigation of Coxiella burnetii contamination in commercial milk and PCR method for the detection of C. burnetii in egg].
abstract: A total of 244 milk samples collected from supermarkets in Tokyo were examined for contamination with Coxiella burnetii. C. burnetii DNA was detected in 131 (53.7%) of the samples by nested PCR. PCR-positive samples were injected into immunosuppressed A/J strain mice. Of the 22 PCR-positive milk samples tested, none resulted in isolation of C. burnetii from the mice. Heat-treatment was sufficient to inactivate C. burnetii in commercial milk. In addition, a PCR detection method for C. burnetii in chicken egg was developed. Egg yolk was added to an equal volume of 1 mol/L of NaCl phosphate buffer and homogenized for removal of protein and lipid. After centrifugal separation, the supernatant was removed, and template DNA in the precipitate was extracted using SDS, proteinase K and NaI. Using such prepared samples, 3.2 x 10(1) C. burnetii particles in 1 g of egg yolk could be detected by nested PCR. All of 200 chicken egg samples collected from supermarkets in Tokyo were negative for C. burnetii by the nested PCR method.
authors.affiliation: Tokyo Metropolitan Institute of Public Health: 3-24-1, Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hirai
authors.forename: Seiji
authors.initials: S
authors.lastname: Kaneko
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakama
authors.forename: Naoto
authors.initials: N
authors.lastname: Ishizaki
authors.forename: Megumi
authors.initials: M
authors.lastname: Odagiri
authors.forename: Akemi
authors.initials: A
authors.lastname: Kai
authors.forename: Kenji
authors.initials: K
authors.lastname: Sadamasu
authors.forename: Takayuki
authors.initials: T
authors.lastname: Shinkai
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Morozumi
mesh_terms: D000818:Animals; D002021:Buffers; D002645:Chickens; D016997:Coxiella burnetii / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D004530:Egg Yolk / Q000382:microbiology; D004531:Eggs / Q000382:microbiology*; D005516:Food Microbiology; D006358:Hot Temperature; D051379:Mice; D008892:Milk / Q000382:microbiology*; D010710:Phosphates / Q000494:pharmacology; D016133:Polymerase Chain Reaction / Q000379:methods*; D014041:Tokyo
chemical_list: D002021:Buffers; D004269:DNA, Bacterial; D010710:Phosphates; C018279:sodium phosphate