title: Chlorinated pesticides (2,4-D and DDT) biodegradation at high concentrations using immobilized Pseudomonas fluorescens.
abstract: Degradation of two chlorinated pesticides (2,4-D and DDT) using a 54-mL glass column packed with tezontle (a low-cost basaltic scoria) was tested. Bacteria were cultured in YPG (yeast, peptone, and glucose) liquid medium at 32 degrees C. The rich medium was pumped during 24 h through the column to inoculate it. Later, the wasted medium was discharged and the pesticide added. Optical densities, TOC, and pesticide concentration were determined. Pesticide removals for 2,4-D (with initial concentration between 100 and 500 mg/L) were about 99%. DDT removal (at initial concentration of up to 150 mg/L) was as high as 55-99%. TOC removals for 2,4-D was in the 36-87% interval, whereas for DDT they were as high as 36-78%.
authors.affiliation: El Colegio de San Luis, AC, SLP, SLP, Mexico.
authors.forename: Germán
authors.initials: G
authors.lastname: Santacruz
authors.forename: Erick R
authors.initials: ER
authors.lastname: Bandala
authors.forename: Luis G
authors.initials: LG
authors.lastname: Torres
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003634:DDT / Q000032:analysis / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006540:Herbicides / Q000032:analysis / Q000378:metabolism*; D007700:Kinetics; D010575:Pesticides / Q000032:analysis / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism*
chemical_list: D006540:Herbicides; D010575:Pesticides; D015084:2,4-Dichlorophenoxyacetic Acid; D003634:DDT