Determination of limonin in rat plasma by liquid chromatography-electrospray mass spectrometry.
A simple, sensitive and selective liquid chromatography coupled with electrospray ionization mass spectrometry (LC/ESI/MS) method for determination of limonin (LM) in rat plasma has been developed and validated. The method had advantages of a single liquid-liquid extraction with ether and high sensitivity. Analyses were conducted at a flow rate of 0.2 ml/min by a gradient elution. The detection utilized selected ion monitoring in the negative ion mode at m/z 460.00 and 423.15 for the deprotonated molecular ions of LM and the internal standard, respectively. The quantitation limit for LM in rat plasma was 1.0 ng/ml. The linearity was also excellent over the concentration range of 1.9-500 ng/ml of LM. The intra- and inter-day precision (relative standard deviation (R.S.D.%)) was lower than 10% and accuracy ranged from 90 to 110%, showing a good reproducibility. This developed method was successfully applied to analysis of LM in biological fluids.
Liang
Yan
Y
China Pharmaceutical University, Shennong Road No. 1, Nanjning 210038, China.
Xie
Lin
L
Liu
Xiao-Dong
XD
Lu
Tong
T
Wang
Guang-Ji
GJ
Hu
Yu-Zhu
YZ
D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid; D005260:Female; D005615:Freezing; D036701:Limonins / Q000097:blood*; D008297:Male; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
D036701:Limonins; C001546:limonin