Glucose trimming of N-glycan in endoplasmic reticulum is indispensable for the growth of Raphanus sativus seedling (kaiware radish).
Recently I found that glycosidase inhibitors such as castanospermine, deoxynojirimycin, swainsonine, 2-acetamindo 2,3-dideoxynojirimycin, and deoxymannojirimycin change the N-glycan structure of root glycoproteins, and that the glucosidase inhibitors castanospermine and deoxynojirimycin suppress the growth of Raphanus sativus seedlings (Mega, T., J. Biochem., 2004). The present study undertook to see whether the growth suppression is due to the inhibition of glucose trimming in endoplasmic reticulum (ER). The study, using three glucosidase inhibitors, castanospermine, N-methyl deoxynojirimycin, and deoxynojirimycin, upon the growth of R. sativus foliage leaf, made clear that glucose trimming is indispensable for plant growth, because the inhibition of glucose trimming correlated with leaf growth. On the other hand, processing inhibition in the Golgi apparatus by other glycosidase inhibitors had little effect on plant growth, although N-glycan processing was disrupted depending on inhibitor specificity. These results suggest that N-glycan processing after glucosidase processing is dispensable for plant growth and cell differentiation.
Mega
Tomohiro
T
Department of Chemistry, Osaka University Graduate School of Science, Machikaneyama-cho 1-1, Toyonaka, Osaka 560-0043, Japan. mega@chem.sci.osaka-u.ac.jp
D017485:1-Deoxynojirimycin / Q000494:pharmacology / Q000031:analogs & derivatives; D000606:Amino Sugars / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D007212:Indolizines / Q000494:pharmacology; D008954:Models, Biological; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D031224:Raphanus / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D012210:Rhamnose / Q000031:analogs & derivatives / Q000494:pharmacology; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D017026:Swainsonine / Q000494:pharmacology; D054836:Indolizidines
D017485:1-Deoxynojirimycin; D000606:Amino Sugars; D004791:Enzyme Inhibitors; D005947:Glucose; D006026:Glycoside Hydrolases; D007212:Indolizines; D009844:Oligosaccharides; D011134:Polysaccharides; D012210:Rhamnose; D017026:Swainsonine; C435774:6-deoxymannojirimycin; C037806:castanospermine; D054836:Indolizidines