title: Labeling of proteins and oligopeptides with luminescent lanthanide(III) chelates.
abstract: Synthesis of a building block that allows introduction of photoluminescent europium(III) and samarium(III) chelates to synthetic oligopeptides on solid phase using standard Fmoc chemistry is described. Upon completion of the oligopeptide synthesis, these conjugates were converted to the corresponding lanthanide(III) chelates by treatment with appropriate lanthanide(III) salt. Also synthesis of a new terpyridine-based europium(III) chelate designed for solution phase protein labeling is demonstrated.
authors.affiliation: PerkinElmer Life and Analytical Sciences, Turku Site, P.O. Box 10, FIN-20101 Turku, Finland.
authors.forename: Lassi
authors.initials: L
authors.lastname: Jaakkola
authors.forename: Jari
authors.initials: J
authors.lastname: Peuralahti
authors.forename: Harri
authors.initials: H
authors.lastname: Hakala
authors.forename: Veli-Matti
authors.initials: VM
authors.lastname: Mukkala
authors.forename: Pertti
authors.initials: P
authors.lastname: Hurskainen
authors.forename: Jari
authors.initials: J
authors.lastname: Hovinen
mesh_terms: D002614:Chelating Agents / Q000737:chemistry*; D007202:Indicators and Reagents; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry
chemical_list: D002614:Chelating Agents; D007202:Indicators and Reagents; D028581:Lanthanoid Series Elements; D049408:Luminescent Agents; D009842:Oligopeptides; D011506:Proteins; D012996:Solutions