title: Efficient transfection of DNA by liposomes formulated with cationic gemini amphiphiles.
abstract: Cationic liposomes formulated with neutral 1,2-dimyristoyl-sn-glycero-3-phosphocholine and cationic gemini surfactants were used for transfecting different cell lines with a reporter gene. The efficiency in the transfection has been correlated to the high extent of DNA condensation observed by circular dichroism, condensation shown to depend heavily on the gemini spacer structure. Transfection efficiency was better than that obtained with a commercial lipofection kit.
authors.affiliation: Istituto di Metodologie Chimiche del CNR and Dipartimento di Chimica, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
authors.forename: Cecilia
authors.initials: C
authors.lastname: Bombelli
authors.forename: Francesca
authors.initials: F
authors.lastname: Faggioli
authors.forename: Paola
authors.initials: P
authors.lastname: Luciani
authors.forename: Giovanna
authors.initials: G
authors.lastname: Mancini
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Sacco
mesh_terms: D000818:Animals; D002082:Butylamines / Q000737:chemistry; D002412:Cations; D002460:Cell Line; D002522:Chlorocebus aethiops; D002942:Circular Dichroism; D003587:Cytomegalovirus / Q000235:genetics; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D005021:Ethylamines / Q000737:chemistry; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D011401:Promoter Regions, Genetic; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D051381:Rats; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000737:chemistry*; D014162:Transfection
chemical_list: D002082:Butylamines; D002412:Cations; D005021:Ethylamines; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D049452:Green Fluorescent Proteins; D004247:DNA; D004134:Dimyristoylphosphatidylcholine