Haplotyping by capillary electrophoresis.
The investigation of the genetic background and phenotype structures of complex diseases, such as cardiovascular or psychiatric disorders and tumors, is one of the most scrutinized fields of the post genomic era. Besides the multiplex analysis of genetic markers and polymorphisms throughout the whole genome, more and more attention is focused on the interaction between the etiological factors of these traits. Haplotype determination, rather than multiplex genotyping seems to be one of the first building blocks of this endeavor. This review focuses on the importance and theoretical background of haplotyping, and summarizes the recent examples of novel and emerging haplotyping techniques by capillary gel electrophoresis based DNA fragment analysis, a powerful tool for the examination of the inheritance of complex traits.
Szantai
Eszter
E
Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest, Hungary.
Ronai
Zsolt
Z
Szilagyi
Agnes
A
Sasvari-Szekely
Maria
M
Guttman
András
A
D004247:DNA / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D005821:Genetic Techniques; D005838:Genotype; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D004247:DNA