Phenol degradation and toxicity assessment upon biostimulation to an indigenous Rhizobium Ralstonia taiwanensis.
This study provides a first attempt from a toxicological perspective to put forward, in general terms and explanations, combined toxic interactions and biostimulation strategy upon nutrient medium to Ralstonia taiwanensis for bioremediation. Dose-response analysis clearly revealed that most of the supplemented nutrients tested (except for gluconic acid) synergistically interact with chronic toxicity to phenol, especially at low doses. Acute toxicity based upon adaptation lag is a more appropriate indicator for comparative analysis of toxicity due to similar toxic ranking at almost all effective concentrations. In addition, comparison upon acute and chronic toxicity for various nutrient media also suggests in parallel that acute toxicity is more significant than chronic toxicity possibly as the result of a more sensitive response of adaptation lag to growth in different media. Feasibility of adding extra nutrient substrates (e.g., phenol, gluconic acid, yeast extract, pyruvic acid, acetic acid, and glycerol) to stimulate proliferation of phenol degraders for better phenol degradation performance was also assessed. The results show that using acetic acid as the augmented nutrient source might be the most feasible biostimulation strategy for phenol degradation.
Chen
Bor-Yann
BY
Department of Chemical and Materials Engineering, National I-Lan University, I-Lan, 260 Taiwan, R.O.C. bychen@niu.edu.tw
Chang
Jo-Shu
JS
D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000009:adverse effects / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005942:Gluconates / Q000494:pharmacology / Q000633:toxicity; D019800:Phenol / Q000378:metabolism* / Q000633:toxicity*; D043367:Ralstonia / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D018675:Toxicity Tests / Q000379:methods; D036401:Toxicity Tests, Chronic / Q000379:methods
D003470:Culture Media; D005942:Gluconates; D019800:Phenol; C030691:gluconic acid