Functional analysis of the PsbP-like protein (sll1418) in Synechocystis sp. PCC 6803.
A recent proteomic analysis of the thylakoid lumen of Arabidopsis thaliana revealed the presence of several PsbP-like proteins, and a homologue to this gene family was detected in the genome of the cyanobacterium Synechocystis sp. PCC 6803 (Schubert M, Petersson UA, Haas BJ, Funk C, Schröder WP, Kieselbach T (2002) J Biol Chem 277, 8354-8365). Using a peptide-directed antibody against this cyanobacterial PsbP-like protein (sll1418) we could show that it was localized in the thylakoid membrane and associated with Photosystem II. While salt washes did not remove the PsbP-like protein from the thylakoid membrane, it was partially lost during the detergent-based isolation of PSII membrane fractions. In total cell extracts this protein is present in the same amount as the extrinsic PsbO protein. We did not see any significant functional difference between the wild-type and a PsbP-like insertion mutant.
Ishikawa
Yasuo
Y
Department of Biochemistry, Umeå University, 90187, Umeå, Sweden.
Schröder
Wolfgang P
WP
Funk
Christiane
C
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D016254:Mutagenesis, Insertional; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D046939:Synechocystis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020524:Thylakoids / Q000737:chemistry / Q000378:metabolism
D001426:Bacterial Proteins; D045332:Photosystem II Protein Complex