Production of eleutherosides in in vitro regenerated embryos and plantlets of Eleutherococcus chiisanensis.
High frequency somatic embryogenesis of Eleutheorcoccus chiisanensis was achieved through suspension culture of embryogenic cells in hormone-free Murashige and Skoog liquid medium supplemented with 30 g sucrose l-1. Cotyledonary somatic embryos were germinated and converted into plantlets using 20 microM: gibberellic acid which were then grown in a 10 l airlift bioreactor. HPLC analysis revealed the accumulation of eleutheroside B, E and E1 in the embryos and plantlets. Thus mass production of embryos and plantlets of E. chiisanensis can be achieved in liquid cultures and the biomass produced may become an alternative source of eleutherosides.
Jeong
J H
JH
Biotechnology Division, Korea Forest Research Institute, Suwon, 441-350, Korea.
Jung
S J
SJ
Murthy
H N
HN
Yu
K W
KW
Paek
K Y
KY
Moon
H K
HK
Choi
Y E
YE
D019149:Bioreactors; D028001:Eleutherococcus / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D010936:Plant Extracts / Q000096:biosynthesis*; D012038:Regeneration; D036226:Seedlings / Q000196:embryology / Q000378:metabolism* / Q000502:physiology; D012639:Seeds / Q000378:metabolism* / Q000502:physiology; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors; D046509:Tissue Culture Techniques
D010936:Plant Extracts; C029073:eleutherosides; D013395:Sucrose