Chemical characterization of exopolysaccharides from Antarctic marine bacteria.
Exopolysaccharides (EPS) may have an important role in the Antarctic marine environment, possibly acting as ligands for trace metal nutrients such as iron or providing cryoprotection for growth at low temperature and high salinity. Ten bacterial strains, isolated from Southern Ocean particulate material or from sea ice, were characterized. Whole cell fatty acid profiles and 16S rRNA gene sequences showed that the isolates included representatives of the genera Pseudoalteromonas, Shewanella, Polaribacter, and Flavobacterium as well as one strain, which constituted a new bacterial genus in the family Flavobacteriaceae. The isolates are, therefore, members of the "Gammaproteobacteria" and Cytophaga-Flexibacter-Bacteroides, the taxonomic groups that have been shown to dominate polar sea ice and seawater microbial communities. Exopolysaccharides produced by Antarctic isolates were characterized. Chemical composition and molecular weight data revealed that these EPS were very diverse, even among six closely related Pseudoalteromonas isolates. Most of the EPS contained charged uronic acid residues; several also contained sulfate groups. Some strain produced unusually large polymers (molecular weight up to 5.7 MDa) including one strain in which EPS synthesis is stimulated by low temperature. This study represents a first step in the understanding of the role of bacterial EPS in the Antarctic marine environment.
Nichols
Carol Mancuso
CM
School of Agricultural Science, University of Tasmania, Hobart 7000, Tasmania, Australia. C.A.Mancuso@utas.edu.au
Lardière
Sandrine Garon
SG
Bowman
John P
JP
Nichols
Peter D
PD
A E Gibson
John
J
Guézennec
Jean
J
D000864:Antarctic Regions; D041963:Bacteroidetes / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003124:Colorimetry; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000737:chemistry / Q000235:genetics*; D008401:Gas Chromatography-Mass Spectrometry; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared; D014574:Uronic Acids / Q000032:analysis
D005227:Fatty Acids; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S; D014574:Uronic Acids