[Study on human leukocyte antigen G1 reducing xeno-cell-rejection by transfecting porcine endothelial cells].
OBJECTIVE
To study whether the porcine endothelial cells (PECs) lines transfected by HLA-G1 can alter the lysis mediated by human peripheral blood mononuclear cell (PBMC) and natural killer cell 92 (NK-92).


METHODS
By use of liposomes pack, the pcDNA3. 0 eukaryotic expression vector carrying HLA-G1 was transfected into PECs. Using indirect immunofluorescence and RT-PCR assays, the HLA-G1 expression in PECs was detected. The alteration of the lysis mediated by PBMC and NK-92 was detected by 51Cr-release assays.


RESULTS
HLA-G1 expression could be detected in PECs after transfection of HLA-G1 at the levels of protein and RNA. It also could be found that the survival rate of transfected PECs was much higher than that of non-transfected PECs, when both of them faced the lysis mediated by human PBMC and NK-92. After transfecting the expression of HLA-G1 could be found in the transfected PECs and the lysis mediated by PBMC and NK-92 to PECs decreased obviously (P<0.05).


CONCLUSIONS
The PECs transfected by HLA-G1 can decrease the NK lysis, so that it may provide us a new thought to inhibit the xeno-cell-rejection.
Xia
Zhenxiong
Z
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, P. R. China.
Han
Junyan
J
Wang
Shusen
S
Zeng
Ronghua
R
Zhu
Min
M
Qi
Honggang
H
Chen
Dong
D
Wu
Xiongwen
X
Gong
Feili
F
Chen
Shi
S
D000818:Animals; D002460:Cell Line; D042783:Endothelial Cells / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation*; D019084:Fluorescent Antibody Technique, Indirect; D005822:Genetic Vectors; D006084:Graft Rejection / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014162:Transfection / Q000379:methods*; D014183:Transplantation, Heterologous
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I