Modulation of base selectivity for a base-discriminating fluorescent nucleobase by addition of mercury ion.
We altered the fluorescence emission selectivity of a base-discriminating fluorescent base, (Py)U, from A-selective to T-selective by the addition of mercury ion. The strong fluorescence from a duplex containing the (Py)U/T base pair was specific to the mercury ion among divalent metal ions, providing a unique method for sensing mercury ions in aqueous solutions.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan. okamoto@sbchem.kyoto-u.ac.jp
Ochi
Yuji
Y
Saito
Isao
I
D020029:Base Pairing; D005456:Fluorescent Dyes / Q000737:chemistry*; D008628:Mercury / Q000032:analysis* / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013941:Thymine; D014498:Uracil
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D014498:Uracil; D008628:Mercury; D013941:Thymine