Glycolysis and perinatal hypoxic-ischemic brain damage.
To ascertain the regulation of glycolysis during perinatal hypoxia-ischemia, 7-day postnatal rats were subjected to unilateral common carotid artery ligation followed by hypoxia with 8% oxygen for up to 90 min. Brain concentrations of glucose, lactate, and key glycolytic intermediates were determined at specific intervals of hypoxia. During hypoxia-ischemia, anaerobic glycolysis increased to approximately 62% of its maximal capacity, which equates to a 135% stimulation of the glycolytic flux. The key regulatory enzymes, hexokinase, phosphofructokinase and pyruvate kinase, were all stimulated during hypoxia-ischemia, and there were no enzymatic rate limitations. The major rate-limiting step for glycolysis was the transport of glucose across the blood-brain barrier into the brain.
Vannucci
Robert C
RC
Department of Pediatrics, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, PA, USA.
Brucklacher
Robert M
RM
Vannucci
Susan J
SJ
D000818:Animals; D000831:Animals, Newborn; D001812:Blood-Brain Barrier / Q000502:physiology; D002339:Carotid Arteries / Q000601:surgery; D004789:Enzyme Activation / Q000502:physiology; D005260:Female; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D006019:Glycolysis / Q000502:physiology*; D006593:Hexokinase / Q000378:metabolism; D002534:Hypoxia, Brain / Q000378:metabolism*; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism*; D019344:Lactic Acid / Q000032:analysis; D008026:Ligation; D008297:Male; D025221:Phosphofructokinases / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D019344:Lactic Acid; D025221:Phosphofructokinases; D006593:Hexokinase; D011770:Pyruvate Kinase; D005947:Glucose