Rapid screening of DOM's metal-binding ability using a fluorescence-based microplate assay.
This note describes the development of a method to rapidly quantify the metal-binding ability of low-volume DOM samples based on their fluorescence properties. The method uses 96-well microplates to screen the fluorescence quenching observed when increasing concentrations of various metals are added to the pH-buffered DOM sample. Only 1.6 mL of DOM sample is required to quantify the binding affinity for each metal and the result is obtained in a few minutes. This study presents results for a surface soil DOM sample for which binding was assessed for Cu2+, Fe2+, Ni2+ at pH 5 and 9.
Dudal
Y
Y
INRA, UMR Climat, Sol et Environnement, Domaine St Paul, Site Agroparc 84914 Avignon Cedex 9, France. Yves.Dudal@avignon.inra.fr
Holgado
R
R
Maestri
G
G
Guillon
E
E
Dupont
L
L
D002244:Carbon / Q000032:analysis; D003300:Copper / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods*; D007501:Iron / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D012987:Soil; D013050:Spectrometry, Fluorescence
D012987:Soil; D002244:Carbon; D003300:Copper; D009532:Nickel; D007501:Iron