Gymnemic acids inhibit rabbit glyceraldehyde-3-phosphate dehydrogenase and induce a smearing of its electrophoretic band and dephosphorylation.
Gymnemic acids (GA) inhibited rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity. Binding of GA to GAPDH was observed by surface plasmon resonance measurement. Incubation of GAPDH with GA induced a smearing of the GAPDH band in SDS-PAGE. The GA-induced smearing was diminished by prior incubation of GA with gamma-cyclodextrin or by GA treatment with NAD. GA treatment did not affect the electrophoretic mobility of glucose-6-phosphate isomerase and dehydrogenase. GA treatment diminished the GAPDH band detected by an antibody to phosphoserine, but did not affect the phosphoserine bands of glucose-6-phosphate isomerase and dehydrogenase. These results indicated that GA specifically induced dephosphorylation of GAPDH.
Izutani
Yusuke
Y
Graduate School of Agriculture, Kyoto Prefectural University, Kyoto 606-8522, Japan.
Murai
Takuya
T
Imoto
Toshiaki
T
Ohnishi
Masatake
M
Oda
Masayuki
M
Ishijima
Sumio
S
D000818:Animals; D004586:Electrophoresis; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000037:antagonists & inhibitors*; D010766:Phosphorylation / Q000187:drug effects; D011817:Rabbits; D012503:Saponins / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*
D012503:Saponins; D014315:Triterpenes; C007898:gymnemic acid; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)