Neuronal and glial cell lines as model systems for studying P2Y receptor pharmacology.
Investigation of the role of extracellular nucleotides in nervous system has been one of the main topics of the P2Y receptor research throughout the years. In parallel to numerous studies on primary culture systems, various neuronal and non-neuronal cell lines have been used to model in vitro the processes mediated by extracellular nucleotides. In this review article, a survey of expression profiles of G protein-coupled P2Y receptor subtypes in nervous-system-derived cell lines is presented, by analysing the receptor expression at the mRNA, protein, and functional level. The variability of receptor expression profiles in established cell lines is further discussed, bringing forward some general properties for neuronal and glial malignant cell lines.
Sak
Katrin
K
Rudolf-Boehm Institute of Pharmacology and Toxicology, and Interdisciplinary Center for Clinical Research, Medical Faculty, University of Leipzig, D-04107 Leipzig, Germany. katrin.sak.001@mail.ee
Illes
Peter
P
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods / Q000639:trends; D002460:Cell Line; D002490:Central Nervous System / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods / Q000639:trends; D006801:Humans; D008959:Models, Neurological; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009480:Neuropharmacology / Q000379:methods* / Q000639:trends; D009711:Nucleotides / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D058472:Receptors, Purinergic P2Y1
D009711:Nucleotides; C546382:P2RY1 protein, human; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D058472:Receptors, Purinergic P2Y1