[Culture and characteristics of human dental papilla cells in vitro].
OBJECTIVE
To culture human dental papilla cells (HDPCs)and to study its cytobiological characters in vitro.


METHODS
HDPCs were isolated and cultured with explant culture technique in vitro; Type I collagen, fibronection and laminin were detected in HDPCs and its secreted matrix with the immunocyto-chemical stain; HDPCs were incubated in mineralized promoting solution containing 10 mmol/L beta-glycerophosphate, 100 mg/L of ascorbic acid and 10 nmol/L dexamethasone supplemented with 10% FBS and the form of mineralized nodules was tested with Alizarin Red S stainning.


RESULTS
Cultured HDPCs in vitro were well growing in DMEM/F12. Type I collagen, fibronection and laminin staining were all positive in both HDPCs and its secreted matrix, and laminin was stained with bunchiness in matrix. Mineralized nodules formed after cultured 27 days by Alizarin Red S stainning.


CONCLUSIONS
HDPCs isolated and cultured are well growing in vitro, have a capability of synthesizing and secreting matrix and in mineralized promoting solution, are able to form mineralizer, so, HDPCs have a capacity of seed cell of tissue engineering regeneration tooth.
Xie
Jia-Min
JM
Key Laboratory of Oral Biomedical Engineering Ministry of Education, Sichuan University, Chengdu 610041, China.
Tian
Wei-Dong
WD
Tang
Wei
W
Chen
Xi-Zhe
XZ
Zheng
Xiao-Hui
XH
Wang
Tao
T
D018929:Cell Culture Techniques; D002478:Cells, Cultured; D024042:Collagen Type I; D003771:Dental Papilla; D003907:Dexamethasone; D005994:Glycerophosphates; D006801:Humans; D066298:In Vitro Techniques; D023822:Tissue Engineering; D014070:Tooth
D024042:Collagen Type I; D005994:Glycerophosphates; D003907:Dexamethasone; C031463:beta-glycerophosphoric acid