Development of array-based technology for detection of HAV using gold-DNA probes.
A sensitive method for detection of Hepatitis A virus (HAV) by utilizing gold-DNA probe on an array was developed. Amino-modified oligodeoxynucleotides at the 5' position were arrayed on an activated glass surface to function as capture probes. Sandwich hybridization occurred among capture probes, the HAV amplicon, and gold nanoparticlesupported oligonucleotide probes. After a silver enhancement step, signals were detected by a standard flatbed scanner or just by naked eyes. As little as 100 fM of HAV amplicon could be detected on the array. Therefore, the array technology is an alternative to be applied in detection of HAV due to its low-cost and high-sensitivity.
Wan
Zhixiang
Z
Department of Biotechnology, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
Wang
Yefu
Y
Li
Shawn Shun-Cheng
SS
Duan
Lianlian
L
Zhai
Jianxin
J
D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry / Q000648:ultrastructure; D006046:Gold / Q000737:chemistry*; D006507:Hepatovirus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D046529:Microscopy, Electron, Transmission; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004279:DNA, Viral; D015345:Oligonucleotide Probes; D006046:Gold