Immobilization of heparin on a silicone surface through a heterobifunctional PEG spacer.
A novel method of immobilizing heparin on a silicone surface through a heterobifunctional PEG spacer was used yield well defined surfaces with highly active surface immobilized heparin and low non-specific protein adsorption. The heparin surface density achieved using this technique was 0.68 microg/cm2. Sessile drop water contact angles showed increased hydrophilicity of the silicone surface after PEG modification and a further decrease in the contact angles following the grafting of heparin. High specificity for ATIII with little fibrinogen adsorption was noted in plasma adsorption studies. This ATIII adsorption was mediated by the heparin layer, since surfaces modified with PEG only did not adsorb significant quantities of AT. The thrombin resistance of the heparin modified surfaces was demonstrably greater as measured by a chromogenic thrombin generation assay. The results suggest that the heterbifunctional PEG linker results in a high density of active heparin on the surfaces.
Chen
Hong
H
Department of Chemical Engineering, McMaster University, Hamilton Ont., Canada L8S 4L7.
Chen
Yang
Y
Sheardown
Heather
H
Brook
Michael A
MA
D000327:Adsorption; D000990:Antithrombin III / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000032:analysis / Q000737:chemistry*; D006493:Heparin / Q000032:analysis / Q000737:chemistry*; D008422:Materials Testing; D011092:Polyethylene Glycols / Q000032:analysis / Q000737:chemistry*; D011485:Protein Binding; D012828:Silicones / Q000032:analysis / Q000737:chemistry*; D013499:Surface Properties
D001798:Blood Proteins; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D012828:Silicones; D011092:Polyethylene Glycols; C013830:baysilon; D000990:Antithrombin III; D006493:Heparin