title: Effects of a high-cholesterol diet on cell behavior in rat periodontitis.
abstract: Studies have shown an association between periodontitis and serum cholesterol levels. We hypothesized that high dietary cholesterol could influence periodontitis as a result of proliferation of the junctional epithelium. Rats were divided into 4 groups. Two groups were fed a regular diet, and 2 groups were fed a high-cholesterol diet. One of each dietary group was treated with periodontitis-inducing agents (lipopolysaccharide and proteases), while the other was treated with pyrogen-free water. Feeding rats with a high-cholesterol diet induced an increase in blood total cholesterol and a decrease in high-density lipoprotein cholesterol. Proliferation of the junctional epithelium with increasing bone resorption was promoted by the consumption of a high-cholesterol diet. High dietary cholesterol further increased the cell-proliferative activity of the junctional epithelium induced by lipopolysaccharide and proteases. These results suggest that high dietary cholesterol can initiate and augment periodontitis in the rat periodontitis model.
authors.affiliation: Department of Oral Health, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8525, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tomofuji
authors.forename: H
authors.initials: H
authors.lastname: Kusano
authors.forename: T
authors.initials: T
authors.lastname: Azuma
authors.forename: D
authors.initials: D
authors.lastname: Ekuni
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D016301:Alveolar Bone Loss / Q000209:etiology; D000704:Analysis of Variance; D000818:Animals; D049109:Cell Proliferation; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000009:adverse effects*; D053938:DNA Fragmentation; D004846:Epithelial Attachment / Q000166:cytology / Q000187:drug effects*; D020287:In Situ Nick-End Labeling; D008297:Male; D010518:Periodontitis / Q000097:blood / Q000209:etiology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D014280:Triglycerides / Q000097:blood
chemical_list: D002791:Cholesterol, Dietary; D018809:Proliferating Cell Nuclear Antigen; D014280:Triglycerides; D002784:Cholesterol