Stage-dependent gene expression profiles during natural killer cell development.
Natural killer (NK) cells develop from hematopoietic stem cells (HSCs) in the bone marrow. To understand the molecular regulation of NK cell development, serial analysis of gene expression (SAGE) was applied to HSCs, NK precursor (pNK) cells, and mature NK cells (mNK) cultured without or with OP9 stromal cells. From 170,464 total individual tags from four SAGE libraries, 35,385 unique genes were identified. A set of genes was expressed in a stage-specific manner: 15 genes in HSCs, 30 genes in pNK cells, and 27 genes in mNK cells. Among them, lipoprotein lipase induced NK cell maturation and cytotoxic activity. Identification of genome-wide profiles of gene expression in different stages of NK cell development affords us a fundamental basis for defining the molecular network during NK cell development.
Kang
Hyung-Sik
HS
Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
Kim
Eun-Mi
EM
Lee
Sanggyu
S
Yoon
Suk-Ran
SR
Kawamura
Toshihiko
T
Lee
Young-Cheol
YC
Kim
Sangsoo
S
Myung
Pyung-Keun
PK
Wang
San Ming
SM
Choi
Inpyo
I
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D020869:Gene Expression Profiling; D015723:Gene Library; D006412:Hematopoietic Stem Cells / Q000502:physiology; D007694:Killer Cells, Natural / Q000502:physiology*; D008071:Lipoprotein Lipase / Q000096:biosynthesis / Q000235:genetics; D051379:Mice
D008071:Lipoprotein Lipase