[Effects of latanoprost eye drops on the proliferation of human Tenon's fibroblasts in tissue culture].
OBJECTIVE
To observe whether there is a direct effect of latanoprost eye drops on the proliferation of cultured human Tenon's capsule fibroblasts (HTCFs) in vitro.


METHODS
Human Tenon's capsule fibroblasts were cultured in vitro. Latanoprost eye drops was serially diluted in order to make nine concentrations ranging from 5.00 microg/ml, 0.50 microg/ml, 0.05 microg/ml to 5 x 10(-8) microg/ml. Fibroblasts growth was evaluated with MTT assay. Morphological changes were observed under phase contrast microscope.


RESULTS
Majority of cultured human Tenon's capsule fibroblasts was dead at the concentration of Latanoprost (5 microg/ml), the A values were significantly decreased (P < 0.01). There were no significant changes of the A values (P > 0.05) in lower concentration (under 0.05 microg/ml) compared with control. there was no statistical difference in proliferation of HTCFs after latanoprost treatment for 24 hours, 48 hours, 72 hours.


CONCLUSIONS
Latanoprost has the toxic effect on the fibroblast at higher concentrations, but there is no influence on the proliferation of HTCFs at the lower concentrations.
Chen
Yu-hong
YH
Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai 200031, China.
Sun
Xing-huai
XH
D000959:Antihypertensive Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D003228:Conjunctiva / Q000166:cytology; D003238:Connective Tissue / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D000077338:Latanoprost; D009883:Ophthalmic Solutions; D011461:Prostaglandins F, Synthetic / Q000494:pharmacology*; D046509:Tissue Culture Techniques
D000959:Antihypertensive Agents; D009883:Ophthalmic Solutions; D011461:Prostaglandins F, Synthetic; D000077338:Latanoprost