[Cytotoxic effect of Shiga toxin type 2 and its B subunit on human renal tubular epithelial cell cultures].
Shiga toxin (Stx)-producing E. coli causing watery diarrhea, hemorrhagic colitis and hemolytic-uremic syndrome (HUS). In Argentina, HUS is the most common cause of acute renal failure in children. The purpose of the present study was to examine the cytotoxicity of Stx type 2 (Stx2) and its B subunit (Stx2B) on human renal tubular epithelial cells (HRTEC), in the presence and absence of inflammatory factors. Cytotoxic effects were assessed in terms of functionality of the epithelium, histological damage, cell viability, protein synthesis and cellular apoptosis. Results show that Stx2 regulates the passage of water through the HRTEC within an incubation period of 1h. Within longer periods, up to 72 hours, the study of morphology, viability, protein synthesis and apoptosis shows that HRTEC were sensitive to the cytotoxic action of Stx2 and Stx2B in a dose- and time-dependent manner. These effects were potentiated by lipopolysaccharides (LPS), IL-1beta, and butirate.
Creydt
Virginia Pistone
VP
Laboratorio de Fisiopatogenia, Departamento de Fisología, Facultad de Medicina, Universidad de Buenos Aires.
Nuñez
Pablo
P
Zotta
Elsa
E
Ibarra
Cristina
C
D000328:Adult; D017209:Apoptosis; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects* / Q000473:pathology; D004926:Escherichia coli / Q000472:pathogenicity; D006463:Hemolytic-Uremic Syndrome / Q000150:complications / Q000382:microbiology / Q000503:physiopathology*; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology*; D021122:Protein Subunits / Q000633:toxicity; D051437:Renal Insufficiency / Q000209:etiology; D022641:Shiga Toxin 2 / Q000633:toxicity*
D021122:Protein Subunits; D022641:Shiga Toxin 2; C450841:shiga toxin 2 B subunit