Induction of transcription within chromosomal DNA loops flanked by MAR elements causes an association of loop DNA with the nuclear matrix.
The spatial organization of an approximately 170 kb region of human chromosome 19, including CD22 and GPR40-GPR43 genes, was studied using in situ hybridization of a set of cosmid and PAC probes with nuclear halos prepared from proliferating and differentiated HL60 cells. The whole region under study was found to be looped out into the nuclear halo in proliferating cells. It is likely that the loop observed was attached to the nuclear matrix via MAR elements present at the flanks of the area under study. Upon dimethyl sulfoxide-induced differentiation of the cells the looped fragment became associated with the nuclear matrix. This change in the spatial organization correlated with the activation of transcription of at least two (CD22 and GPR43) genes present within the loop. The data obtained are discussed in the framework of the hypothesis postulating that the spatial organization of chromosomal DNA is maintained via constitutive (basic) and facultative (transcription-related) interactions of the latter with the nuclear matrix.
Iarovaia
Olga V
OV
Laboratory of Structural and Functional Organization of Chromosomes, Institute of Gene Biology RAS, Vavilov Street 34/5, 117984 Moscow, Russia.
Akopov
Sergey B
SB
Nikolaev
Lev G
LG
Sverdlov
Eugene D
ED
Razin
Sergey V
SV
D001483:Base Sequence; D002454:Cell Differentiation; D002875:Chromosomes / Q000737:chemistry*; D002888:Chromosomes, Human, Pair 19 / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D015533:Transcriptional Activation
D004247:DNA; D004250:DNA Topoisomerases, Type II