title: [Effects of astragalus polysaccharides-chitosan/polylactic acid composite material on biological behavior of canine bone marrow stromal cells cultured in vitro].
abstract: OBJECTIVE
  To observe the biological behavior of canine bone marrow stromal cells (BMSCs) cultured in vitro with the astragalus polysaccharides-chitosan/polylactic acid (AP-C/PLA) and with the chitosan/polylactic acid (C/PLA) and to find a suitable compound material for periodontal tissue engineering.
  
  
  METHODS
  BMSCs (induced 14 days by 50 mg/L vitamine C, 10(-8) mol/L dexamethasone, 10 mmol/L beta-sodium glycerylphosphate) were cultured on AP-C/PLA or C/PLA for 5 days respectively. The BMSCs attachment and the morphology were observed with scanning electronic microscope and the combining rates were counted. Type I collagen synthesis was examined with immunohistochemistry staining and the content of osteocalin was determined with radio-immunological method.
  
  
  RESULTS
  Combining rates, type I collagen synthesis, and the content of osteocalin of BMSCs on AP-C/PLA were significantly higher than those on C/PLA.
  
  
  CONCLUSIONS
  AP-C/PLA may promote the BMSC proliferation, differentiation and extracellular matrix synthesis, and it can be used as a good scaffold material for bone tissue engineering.
authors.affiliation: Department of Periodontology; Xiangya Hospital, Central South University, Changsha, China. jianxinchun@hotmail.com
authors.forename: Chun-Jiao
authors.initials: CJ
authors.lastname: Xu
authors.forename: Xin-Chun
authors.initials: XC
authors.lastname: Jian
authors.forename: Jie-Ying
authors.initials: JY
authors.lastname: Peng
authors.forename: Feng
authors.initials: F
authors.lastname: Guo
authors.forename: Bai-Ying
authors.initials: BY
authors.lastname: Huang
authors.forename: Cheng-Dong
authors.initials: CD
authors.lastname: Xiong
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Pan
mesh_terms: D000818:Animals; D027885:Astragalus propinquus; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D024042:Collagen Type I / Q000096:biosynthesis; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D019344:Lactic Acid / Q000494:pharmacology*; D008297:Male; D015675:Osteocalcin / Q000096:biosynthesis; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology*; D023822:Tissue Engineering
chemical_list: D024042:Collagen Type I; D004365:Drugs, Chinese Herbal; D011091:Polyesters; D011108:Polymers; D011134:Polysaccharides; D015675:Osteocalcin; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan