New genotype-phenotype linkages for directed evolution of functional proteins.
The key practical consideration in directed evolution of functional biomolecules is the linkage of genotype and phenotype. In vitro selections offer the potential to select from libraries with up to 10(10)-10(14) members, with fewer constraints than current cell-based selections. New approaches such as mRNA display, ribosome display and in vitro compartmentalisation have complementary areas of application in selections for binding or catalysis.
Leemhuis
Hans
H
Department of Biochemistry, Cambridge University, Cambridge CB2 1GA, UK.
Stein
Viktor
V
Griffiths
Andrew D
AD
Hollfelder
Florian
F
D019020:Directed Molecular Evolution; D005838:Genotype; D010641:Phenotype; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D011506:Proteins