An experimental study on the therapeutic effect of consecutive low-dose cisplatin with caffeine in sarcoma-bearing mice.
The aims of this study were to investigate the anti-tumor effects of consecutive low-dose cisplatin (LD-CDDP) in comparison with single high-dose CDDP (HD-CDDP) in combination with caffeine.


METHODS
Human fibrosarcoma cells (HT-1080) were transplanted in BALB/C-nu mice. According to the administration of CDDP and caffeine, 5 groups were defined: HD-CDDP, LD-CDDP, HD-CDDP +caffeine, LD-CDDP +caffeine and control group. The total dose of CDDP was 3.5 or 6 mg/kg. CDDP was injected i.p. bolus (HD-CDDP) or divided into 5 days (LD-CDDP). Caffeine was injected i.p. at a dose of 60 mg/kg twice a day for 4 days.


RESULTS
Significant inhibition of tumor growth and prolongation of survival time were recognized in the HD- and LD-CDDP groups with and without caffeine compared to the control group. The caffeine-assisted groups had no advantage compared to the CDDP alone groups.


CONCLUSIONS
The effects of LD-CDDP were similar to the effect of HD-CDDP.
Takeda
Akira
A
Department of Orthopedic Surgery, Fukushima Medical University, School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan. takeda@fmu.ac.jp
Tsuchiya
Hiroyuki
H
Tajino
Takahiro
T
Kikuchi
Shin-Ichi
S
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000009:adverse effects / Q000494:pharmacology*; D002110:Caffeine / Q000008:administration & dosage / Q000009:adverse effects / Q000097:blood; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000008:administration & dosage / Q000009:adverse effects; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005354:Fibrosarcoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D023041:Xenograft Model Antitumor Assays
D002110:Caffeine; D002945:Cisplatin