title: Determination of major ginsenosides in Panax quinquefolius (American ginseng) using high-performance liquid chromatography.
abstract: In order to determine the active ingredients in root extracts of Panax quinquefolius (American ginseng), a gradient HPLC method involving UV photodiode array detection was applied to separate and quantify simultaneously the ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf and Rg1. All ginseng saponins were baseline-resolved under the selected conditions, and the detection limits were 1.0 microg/mL or less. The method has been applied to analyse ginsenosides extracted from American ginseng cultivated in both Wisconsin and Illinois. Ginsenosides Re and Rb1 were the two main ginseng saponins in the root. The amounts of Re in 5- and 7-year Illinois-cultivated samples were greater than those found in ginseng cultivated for 3 or 4 years in Wisconsin, whereas the levels of Rb1 were greater in the younger Wisconsin samples.
authors.affiliation: Tang Center for Herbal Medicine Research, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Anbao
authors.initials: A
authors.lastname: Wang
authors.forename: Chong-Zhi
authors.initials: CZ
authors.lastname: Wang
authors.forename: Ji-An
authors.initials: JA
authors.lastname: Wu
authors.forename: Joachim
authors.initials: J
authors.lastname: Osinski
authors.forename: Chun-Su
authors.initials: CS
authors.lastname: Yuan
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry; D036145:Ginsenosides / Q000302:isolation & purification*; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides