title: Neuroendocrine carcinoma in the nasal cavity of ten dogs.
abstract: Neuroendocrine (NE) carcinoma was diagnosed in 10 dogs. In six cases examined by cephalometric radiography and computerized tomography, a large mass was seen to fill the nasal cavity. Histopathologically, sheets, nests or ribbons of neoplastic cells were separated by delicate or thick fibrovascular stroma. The neoplastic cells were round, oval, or spindle-shaped; cytoplasmic granules and hyperchromatic nuclei with prominent nucleoli were present. Neoplastic cells were invariably immunohistochemically positive for cytokeratin (CK) AE1/AE3, neuron-specific enolase, chromogranin A and vasoactive intestinal polypeptide. Eight dogs were positive for S100 protein, seven for synaptophysin, five for protein gene product 9.5, two for somatostatin, and one for Leu-7. Immunolabelling gave negative results for CK 8, CK 19, calcitonin, calcitonin gene-related polypeptide, neurofilaments, serotonin, gastrin and glial fibrillary acidic protein. Ultrastructurally, the neoplastic cells contained a large number of round, membrane-bounded, densely-cored granules corresponding to neurosecretory granules. These observations were consistent with the neuroendocrine nature of the carcinomas.
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, 582-1 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sako
authors.forename: Y
authors.initials: Y
authors.lastname: Shimoyama
authors.forename: Y
authors.initials: Y
authors.lastname: Akihara
authors.forename: T
authors.initials: T
authors.lastname: Ohmachi
authors.forename: K
authors.initials: K
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Kadosawa
authors.forename: T
authors.initials: T
authors.lastname: Nakade
authors.forename: E
authors.initials: E
authors.lastname: Uchida
authors.forename: M
authors.initials: M
authors.lastname: Okamoto
authors.forename: K
authors.initials: K
authors.lastname: Hirayama
authors.forename: H
authors.initials: H
authors.lastname: Taniyama
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D018278:Carcinoma, Neuroendocrine / Q000737:chemistry / Q000473:pathology / Q000662:veterinary*; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004283:Dog Diseases / Q000000981:diagnostic imaging / Q000401:mortality / Q000473:pathology*; D004285:Dogs; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D009296:Nasal Cavity / Q000000981:diagnostic imaging / Q000473:pathology*; D009490:Neurosecretory Systems / Q000648:ultrastructure; D009669:Nose Neoplasms / Q000737:chemistry / Q000473:pathology / Q000662:veterinary*; D015996:Survival Rate; D014057:Tomography, X-Ray Computed / Q000662:veterinary
chemical_list: D014408:Biomarkers, Tumor