title: Real-time fluorescence polymerase chain reaction (PCR) identification of Streptococcus pneumoniae from pleural fluid and tissue.
abstract: A diagnosis of pneumococcal pneumonia and empyema was established by real-time fluorescence polymerase chain reaction (PCR) performed in pleural fluid and tissue samples from a child in whom cultures were negative. This case demonstrates that the use of solid-phase nucleic acid purification technology and real-time fluorescence PCR have an application in the rapid diagnosis of S. pneumoniae pneumonia.
authors.affiliation: Department of Pathology and Laboratory Medicine, UCLA Medical Center, USA.
authors.forename: Melinda D
authors.initials: MD
authors.lastname: Poulter
authors.forename: Jaime G
authors.initials: JG
authors.lastname: Deville
authors.forename: James D
authors.initials: JD
authors.lastname: Cherry
authors.forename: Alan M
authors.initials: AM
authors.lastname: Shapiro
authors.forename: James C
authors.initials: JC
authors.lastname: McAvin
mesh_terms: D015373:Bacterial Typing Techniques; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D016724:Empyema, Pleural / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D005453:Fluorescence; D006801:Humans; D010994:Pleura / Q000382:microbiology*; D010996:Pleural Effusion / Q000382:microbiology*; D011018:Pneumonia, Pneumococcal / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial