title: [Analysis of nine kinds of sweeteners in foods by LC/MS].
abstract: A simple and rapid method for the simultaneous determination of nine kinds of sweeteners (acesulfame potassium, AK; sucralose, SUC; saccharin, SA; cyclamate, CYC; aspartame, APM; dulcin, DU; glycyrrhizic acid, GA; stevioside, STV; rebaudioside A, REB) in various foods by high-performance liquid chromatography-electrospray mass spectrometry (LC/ESI-MS) was developed. The LC separation was performed on a ZORBAX Eclipse XDB-C18 (2.1 mm x 150 mm) with a mobile phase of 5 mmol/L dibutylammonium acetate (DBAA) and acetonitrile-water (8: 2). Mass spectral acquisition was done in the negative ion mode by applying selected ion monitoring (SIM). The sweeteners were extracted from foods with 0.08 mol/L phosphate buffer (pH 7.0)- ethanol (1:1), and the extract was cleaned up on a Sep-pak Vac C18 cartridge after the addition of tetrabutylammonium bromide and phosphate buffer (pH 3.0). The recovery of the nine kinds of sweeteners from five kinds of foods fortified at the level 0.01 g/kg, 0.05 g/kg and 0.20 g/kg was 75.7-109.2%, and the between-day SD values were 0.5-10.9%. The quantification limits of AK, SA, CYC, APM and STV were 0.001 g/kg, and those of SUC, DU, GA and REB were 0.005 g/kg. A recovery test from each cleaned-up sample solution was necessary to detect ionization suppression.
authors.affiliation: Laboratory, Japanese Consumers' Co-operative Union, Nishiki-cho, Warabi-shi, Saitama 335-0005, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Koyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yoshida
authors.forename: Nobutake
authors.initials: N
authors.lastname: Uchibori
authors.forename: Ichirou
authors.initials: I
authors.lastname: Wada
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Akiyama
authors.forename: Tamami
authors.initials: T
authors.lastname: Sasaki
mesh_terms: D001218:Aspartame / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003494:Cyclamates / Q000032:analysis; D045786:Diterpenes, Kaurane / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D005960:Glucosides / Q000032:analysis; D019695:Glycyrrhizic Acid / Q000032:analysis; D010671:Phenylurea Compounds / Q000032:analysis; D012439:Saccharin / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013395:Sucrose / Q000031:analogs & derivatives / Q000032:analysis; D013549:Sweetening Agents / Q000032:analysis*; D013843:Thiazines / Q000032:analysis
chemical_list: D003494:Cyclamates; D045786:Diterpenes, Kaurane; D005960:Glucosides; D010671:Phenylurea Compounds; D013549:Sweetening Agents; D013843:Thiazines; C012043:stevioside; D013395:Sucrose; D019695:Glycyrrhizic Acid; C004352:dulcin; C026285:trichlorosucrose; C025474:rebaudioside A; D012439:Saccharin; C006362:acetosulfame; D001218:Aspartame