Staphylococcus aureus peptidoglycan is a toll-like receptor 2 activator: a reevaluation.
Since the ability of peptidoglycan (PGN) to activate Toll-like receptor 2 (TLR2) was recently questioned, we reevaluated activation of TLR2 by PGN. Polymeric soluble or insoluble Staphylococcus aureus PGN, repurified by sodium dodecyl sulfate or phenol extraction, activated TLR2 at 0.1 to 1 or 10 mug/ml, respectively, and induced tumor necrosis factor alpha production. The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator.
Dziarski
Roman
R
Northwest Center for Medical Education, Indiana University School of Medicine, 3400 Broadway, Gary, IN 46408, USA. rdziar@iun.edu
Gupta
Dipika
D
D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism*; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors
D008562:Membrane Glycoproteins; D010457:Peptidoglycan; D011956:Receptors, Cell Surface; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors