Measuring the affinity of a radioligand with its receptor using a rotating cell dish with in situ reference area.
This report describes a semi-automated method for the measurement of affinity of radiolabeled ligands interacting with cell-surface receptors on intact cancer cells. The method saves labor time and reagents compared to common manual measurements. A complete affinity measurement can be performed in one cell dish by using a target cell area and a reference area and repeatedly measure the differential activity (i.e. target activity-reference activity). The affinities obtained for different ligand-receptor interactions agreed with affinities reported in the literature.
Björke
Henrik
H
Division of Biomedical Radiation Sciences, Uppsala University, Sweden.
Andersson
Karl
K
D000906:Antibodies / Q000378:metabolism; D001667:Binding, Competitive; D045744:Cell Line, Tumor; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D011869:Radioligand Assay / Q000295:instrumentation* / Q000379:methods; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
D000906:Antibodies; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D007457:Iodine Radioisotopes; D018719:Erb-b2 Receptor Tyrosine Kinases