title: Probiotics reduce enterohemorrhagic Escherichia coli O157:H7- and enteropathogenic E. coli O127:H6-induced changes in polarized T84 epithelial cell monolayers by reducing bacterial adhesion and cytoskeletal rearrangements.
abstract: The aim of this study was to determine if probiotics reduce epithelial injury following exposure to Escherichia coli O157:H7 and E. coli O127:H6. The pretreatment of intestinal (T84) cells with lactic acid-producing bacteria reduced the pathogen-induced drop in transepithelial electrical resistance. These findings demonstrate that probiotics prevent epithelial injury induced by attaching-effacing bacteria.
authors.affiliation: Gastroenterology and Nutrition, Room 8409, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada. sherman@sickkids.ca
authors.forename: Philip M
authors.initials: PM
authors.lastname: Sherman
authors.forename: Kathene C
authors.initials: KC
authors.lastname: Johnson-Henry
authors.forename: Helen P
authors.initials: HP
authors.lastname: Yeung
authors.forename: Peter S C
authors.initials: PS
authors.lastname: Ngo
authors.forename: Jacques
authors.initials: J
authors.lastname: Goulet
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Tompkins
mesh_terms: D000185:Actinin / Q000378:metabolism; D001422:Bacterial Adhesion / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology; D004927:Escherichia coli Infections / Q000378:metabolism / Q000628:therapy; D019453:Escherichia coli O157 / Q000378:metabolism*; D006801:Humans; D007779:Lactobacillus acidophilus / Q000378:metabolism; D019936:Probiotics / Q000378:metabolism*
chemical_list: D000185:Actinin