A new RNA synthetic method with a 2'-O-(2-cyanoethoxymethyl) protecting group.
A novel method for the synthesis of RNA oligomers with 2-cyanoethoxymethyl (CEM) as the 2'-hydroxyl protecting group has been developed. The new method allows the synthesis of oligoribonucleotides with an efficiency and final purity comparable to that obtained in DNA synthesis. [structure: see text]
Ohgi
Tadaaki
T
Discovery Research Laboratories, Nippon Shinyaku Co. Ltd., 3-14-1 Sakura, Tsukuba City, Ibaraki 305-0003, Japan. t.ohgi@po.nippon-shinyaku.co.jp
Masutomi
Yutaka
Y
Ishiyama
Kouichi
K
Kitagawa
Hidetoshi
H
Shiba
Yoshinobu
Y
Yano
Junichi
J
D001483:Base Sequence; D008956:Models, Chemical; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009570:Nitriles / Q000737:chemistry*; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
D009570:Nitriles; D012313:RNA