[The expressions of K-ras in human laryngeal squamous cell carcinoma cell lines (Hep-2) and its significance].
OBJECTIVE
K-ras oncogene activation has been common in pancreatic carcinomas and colon cancer and lung cancer. Some researchers in other countries have reported that human laryngeal squamous cell carcinoma is unrelated to K-ras gene activation. This study aims to present our research on the expression of K-ras in human laryngeal squamous cell carcinoma cell lines (Hep-2) and its significance for establishing a solid foundation for further study of the relativity of human laryngeal squamous cell carcinoma and K-ras gene point mutations.


METHODS
We have measured the expression of K-ras in human laryngeal squamous cell carcinoma cell lines (Hep-2) and in human pancreatic carcinoma cell lines (MIAPaCa-2) with RT-PCR technology.


RESULTS
The expression of K-ras mRNA in Hep-2 and MIAPaCa-2 is strong and positive.


CONCLUSIONS
The expression of K-ras mRNA in human laryngeal squamous cell carcinoma cell lines (Hep-2) is positive. Development of laryngeal carcinoma could be in connection with the activation of K-ras gene point mutation.
Chen
Xiong
X
Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. chenxiong716@yahoo.com.cn
Kong
Weijia
W
Cai
Guanglian
G
Zhang
Sulin
S
Zhang
Dan
D
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D017354:Point Mutation; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger