Inhibition of intestinal sodium-dependent inorganic phosphate transport by fibroblast growth factor 23.
The mechanisms by which fibroblast growth factor 23 (FGF23) alters inorganic phosphate (Pi) homeostasis is not entirely clear. In the present study, we examined the effect of FGF23 on intestinal sodium-dependent Pi transport in mice. Injection of FGF23(R179Q) markedly reduced serum Pi and 1,25(OH)2D3 levels in normal mice. Those animals show the reduction of intestinal sodium-dependent Pi transport activity and the amount of type IIb sodium-dependent Pi cotransporter (type IIb NaPi) protein in the brush border membrane vesicles. In vitamin D receptor null mice (VDR-/-), FGF23(R179Q) had no effect on intestinal sodium-dependent Pi transport activity and type IIb NaPi protein levels. The present study suggests that FGF23(R179Q) reduces intestinal sodium-dependent Pi transport activity and type IIb NaPi protein levels by a mechanism that is dependent on VDR.
Miyamoto
Ken-ichi
K
Department of Molecular Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima City, Japan. miyamoto@nutr.med.tokushima-u.ac.jp
Ito
Mikiko
M
Kuwahata
Masashi
M
Kato
Shigeaki
S
Segawa
Hiroko
H
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000097:blood; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology*; D015151:Immunoblotting; D007421:Intestine, Small / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010281:Parathyroid Hormone / Q000097:blood; D010710:Phosphates / Q000378:metabolism*; D050608:Sodium-Phosphate Cotransporter Proteins; D027981:Symporters / Q000378:metabolism; D014807:Vitamin D / Q000097:blood
C000717428:Fgf23 protein, mouse; D010281:Parathyroid Hormone; D010710:Phosphates; D050608:Sodium-Phosphate Cotransporter Proteins; D027981:Symporters; D014807:Vitamin D; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23; D002118:Calcium