Ectopic expression of the human adenine nucleotide translocase, isoform 3 (ANT-3). Characterization of ligand binding properties.
The adenine nucleotide translocase (ANT) is a key component in maintaining cellular energy homeostasis, and has also been implicated in formation of the mitochondrial permeability transition pore. Human ANT-3 was cloned from a human heart cDNA library and expressed as a histidine-tagged fusion protein in the mitochondria of the Trichoplusia ni. cell line. Overexpression resulted in a concomitant decrease in the endogenous ANT content, allowing for the characterization of binding of known ANT ligands to the human protein. Binding affinities for bongkrekic acid (BKA), ADP, and atractyloside (ATR) were measured in mitochondria from the human ANT-3 expressing cell line, and compared to similar preparations from bovine heart mitochondria by use of a novel radioiodinated derivative of ATR. Binding to ANT-3 by the high affinity inhibitors BKA and ATR, as well as the lower affinity natural ligand ADP, was similar to that measured in bovine heart mitochondria, and to that previously reported for mammalian heart mitochondria. Characterizations such as these of human ANT isoforms may lead to drug development for enhanced mitochondrial function and cellular viability.
Carroll
A K
AK
MitoKor, Inc., San Diego, CA 92121, USA. acarroll@amylin.com
Clevenger
W R
WR
Szabo
T
T
Ackermann
L E
LE
Pei
Y
Y
Ghosh
S S
SS
Glasco
S
S
Nazarbaghi
R
R
Davis
R E
RE
Anderson
C M
CM
D033781:Adenine Nucleotide Translocator 3 / Q000235:genetics* / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D001865:Bongkrekic Acid / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D004734:Energy Metabolism; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007700:Kinetics; D008024:Ligands; D008929:Mitochondria, Heart / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism*; D009036:Moths; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D033781:Adenine Nucleotide Translocator 3; D007457:Iodine Radioisotopes; D008024:Ligands; D024101:Mitochondrial Proteins; D011994:Recombinant Proteins; D001865:Bongkrekic Acid; D001278:Atractyloside; D000244:Adenosine Diphosphate