title: In vitro antileukaemic activity of extracts from berry plant leaves against sensitive and multidrug resistant HL60 cells.
abstract: The aim of the present study was to determine in vitro antileukaemic activity of extracts obtained from selected berry plant leaves (Fragaria x ananassa Duch. cv Elsanta, raspberry Rubus ideus L. cv Polana and blueberry Vaccinium corymbosum L. cv Bluecrop) against promyelocytic HL60 cell line and its multidrug resistant sublines exhibiting two different MDR phenotypes: HL60/VINC (overexpressing P-glycoprotein) and HL60/DOX (overexpressing MRP1 protein). It was found that the blueberry extract was the most efficient against sensitive HL60 cell line (about 2-fold more active than strawberry and raspberry extracts) but presented much lower activity towards resistant cells. In contrast, strawberry and raspberry extracts exhibited the high cytotoxic activity against sensitive leukaemia HL60 cell line as well as its MDR sublines. The values of resistance factor (RF) found for these extracts were very low lying in the range 0.32/2.0.
authors.affiliation: Department of Processing and Storage of Plant Raw Materials, Agricultural Academy of Szczecin, 17 Słowackiego st, 71-434 Szczecin, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Skupień
authors.forename: Jan
authors.initials: J
authors.lastname: Oszmiański
authors.forename: Dorota
authors.initials: D
authors.lastname: Kostrzewa-Nowak
authors.forename: Jolanta
authors.initials: J
authors.lastname: Tarasiuk
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis / Q000235:genetics; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D029798:Blueberry Plants; D049109:Cell Proliferation / Q000187:drug effects; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004610:Ellagic Acid / Q000032:analysis; D031985:Fragaria; D005707:Gallic Acid / Q000032:analysis; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000096:biosynthesis / Q000235:genetics; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000032:analysis
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000972:Antineoplastic Agents, Phytogenic; D004610:Ellagic Acid; D005707:Gallic Acid; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D010636:Phenols; D010936:Plant Extracts; D011794:Quercetin; C421109:multidrug resistance-associated protein 1