[The photodynamic therapy effect of a new cationic porphyrin on human laryngeal cancer Hep-2 cell in vitro].
OBJECTIVE
To investigate the photodynamic therapy (PDT) effect of a new kind of cationic porphyrin on human laryngeal cancer Hep-2 cell in vitro.


METHODS
A new cationic porphyrin (5-(4-methoxycarbonylphenyl)-10, 15, 20-tris(4-N-methylpyridiniumyl)porphyrin triiodide ) was synthesized. The Hep-2 cells were treated with three different concentrations of porphyrin (10(-7) mol/L, 10(-6) mol/L, 10(-5) mol/L) followed by irradiation of different visible light (0, 0.5, 1.0, 1.5, 2.0 h). MTT and TUNEL were applied to measure the inhibitory effect of porphyrin on the Hep-2 cells growth, inhibitory rate and induced apoptosis rate.


RESULTS
Under definite condition, the cantionic porphyrin concentrations had a significant inhibitory effect and induced apoptosis effect. The effect depended greatly on the doses of the concentration and the time of irradiation of light. At the highest concentration of porphyrin (10(-5) mol/L), the growth inhibitory rate were 1.18%, 35.21%, 58.59%, 70.42%, 84.34% respectively and the apoptosis rate were 3.30% +/- 0.29%, 29.41% +/- 2.12%, 32.30% +/- 2.26%, 34.52% +/- 3.11% in different visible light (0.05, 1.0, 1.5, 2.0 h), respectively. Under high irradiation energy, it can causesome Hep-2 cells death directly.


CONCLUSIONS
Porphyrin has a distinctive killing effect on Hep-2 cells and correlated with the doses and the irradiation time.
Yan
Fubo
F
Department of Otolaryngology-Head and Neck Surgery,Renmin Hospital,Wuhan University, Wuhan, 430060, China. yfb85680076@sina.com
Tao
Zezhang
Z
Jia
Tao
T
Xiao
Bokui
B
Chi
Huaming
H
Chen
Shiming
S
D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010778:Photochemotherapy; D011166:Porphyrins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D011166:Porphyrins