title: Plague bacteria target immune cells during infection.
abstract: The plague is caused by the bacterium Yersinia pestis. Plague bacteria are thought to inject effector Yop proteins into host cells via the type III pathway. The identity of the host cells targeted for injection during plague infection is unknown. We found, using Yop beta-lactamase hybrids and fluorescent staining of live cells from plague-infected animals, that Y. pestis selected immune cells for injection. In vivo, dendritic cells, macrophages, and neutrophils were injected most frequently, whereas B and T lymphocytes were rarely selected. Thus, it appears that Y. pestis disables these cell populations to annihilate host immune responses during plague.
authors.affiliation: Department of Microbiology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Melanie M
authors.initials: MM
authors.lastname: Marketon
authors.forename: R William
authors.initials: RW
authors.lastname: DePaolo
authors.forename: Kristin L
authors.initials: KL
authors.lastname: DeBord
authors.forename: Bana
authors.initials: B
authors.lastname: Jabri
authors.forename: Olaf
authors.initials: O
authors.lastname: Schneewind
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D005434:Flow Cytometry; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D017737:Macrophages, Peritoneal / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D010930:Plague / Q000276:immunology / Q000382:microbiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D014169:Transformation, Bacterial; D015010:Yersinia pestis / Q000378:metabolism / Q000472:pathogenicity*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D011993:Recombinant Fusion Proteins; C063239:yopE protein, Yersinia; C060772:yopM protein, Yersinia