title: Yeast extract and methyl jasmonate-induced silymarin production in cell cultures of Silybum marianum (L.) Gaertn.
abstract: The biosynthesis of the flavonolignan silymarin, a constitutive compound of the fruits of Silybum marianum with strong antihepatotoxic and hepatoprotective activities, is severely reduced in cell cultures of this species. It is well known that elicitation is one of the strategies employed to increase accumulation of secondary metabolites. Our study here reports on the effect of several compounds on the production of silymarin in S. marianum cultures. Yeast extract (YE), chitin and chitosan were compared with respect to their effects on silymarin accumulation in S. marianum suspensions and only yeast extract stimulated production. Jasmonic acid (JA) potentiated the yeast extract effect. One of the jasmonic acid derivatives, methyl jasmonate (MeJA), strongly promoted the accumulation of silymarin. Methyl jasmonate acted in a number of steps of the metabolic pathway of flavonolignans and its stimulating effect was totally dependent of "de novo" protein synthesis. Chalcone synthase (CHS) activity was enhanced by methyl jasmonate; however there did not appear to be a temporal relationship between silymarin accumulation and increase in enzyme activity. Also, this increase was not blocked by the protein synthesis inhibitor cycloheximide (CH). This study indicates that elicitor treatment promotes secondary metabolite production in S. marianum cultures and that jasmonic acid and its functional analogue plays a critical role in elicitation.
authors.affiliation: Departamento de Fisiología Vegetal, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain.
authors.forename: M Angeles
authors.initials: MA
authors.lastname: Sánchez-Sampedro
authors.forename: Jorge
authors.initials: J
authors.lastname: Fernández-Tárrago
authors.forename: Purificación
authors.initials: P
authors.lastname: Corchete
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000217:Acyltransferases / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D002686:Chitin / Q000494:pharmacology; D048271:Chitosan / Q000494:pharmacology; D045424:Complex Mixtures / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D020944:Silybum marianum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology*; D020156:Salicylic Acid / Q000494:pharmacology; D012838:Silymarin / Q000032:analysis / Q000096:biosynthesis*; D015003:Yeasts / Q000737:chemistry
chemical_list: D000085:Acetates; D045424:Complex Mixtures; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D012838:Silymarin; D002686:Chitin; C072239:methyl jasmonate; D048271:Chitosan; D000217:Acyltransferases; C020141:flavanone synthetase; D020156:Salicylic Acid