title: Molecular characterization of Agrotis segetum nucleopolyhedrovirus from Poland.
abstract: The turnip moth, Agrotis segetum (Lepidoptera, Noctuidae), is an important pest insect in Europe, Asia, and Africa. We have genetically characterized and classified a nucleopolyhedrovirus isolated from A. segetum larvae in Poland (AgseNPV-P). The restriction pattern of AgseNPV-P was distinct from an isolate from England/France (AgseNPV-UK and AgseNPV-F). Sequence analysis of three conserved baculovirus genes, polyhedrin, lef-8 and pif-2, revealed that AgseNPV-P differs substantially from the already described NPVs isolated from A. segetum and possibly represents a new NPV species. Phylogenetic analysis placed AgseNPV-P among group II NPVs and showed the closest relationship to Agrotis ipsilon (Agip) NPV and Spodoptera exigua (Se) MNPV.
authors.affiliation: Laboratory of Virology, Wageningen University, Binnenhaven 11, Wageningen, 6709 PD, The Netherlands.
authors.forename: Agata
authors.initials: A
authors.lastname: Jakubowska
authors.forename: Monique M
authors.initials: MM
authors.lastname: van Oers
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Ziemnicka
authors.forename: Jerzy J
authors.initials: JJ
authors.lastname: Lipa
authors.forename: Just M
authors.initials: JM
authors.lastname: Vlak
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D010802:Phylogeny; D011044:Poland