The impact of fluid mechanical stress on Corynebacterium glutamicum during continuous cultivation in an agitated bioreactor.
The effect of mechanical stresses generated by an extreme agitation intensity or a high aeration rate on growth parameters and cell physiology were studied during continuous cultivation of the Gram-positive bacterium Corynebacterium glutamicum. It is concluded that variations in agitation, aeration rate, or dO2 concentrations down to about 1% of saturation do not damage the bacterial cells or cause a significant change in physiological response, as measured by flow cytometry, even though the cell size was slightly reduced.
Chamsartra
Saethawat
S
BBERG Bangsaen Biochemical Engineering Research Group, Department of Biology, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand.
Hewitt
Christopher J
CJ
Nienow
Alvin W
AW
D000332:Aerobiosis; D000465:Algorithms; D001431:Bacteriological Techniques / Q000379:methods; D019149:Bioreactors / Q000382:microbiology*; D048230:Corynebacterium glutamicum / Q000254:growth & development* / Q000648:ultrastructure; D005434:Flow Cytometry; D008855:Microscopy, Electron, Scanning; D013314:Stress, Mechanical