title: Extensive modulation of a set of microRNAs in primary glioblastoma.
abstract: MicroRNAs (miRNAs) are short non-coding RNA molecules playing regulatory roles in animals and plants by repressing translation or cleaving RNA transcripts. The specific modulation of several microRNAs has been recently associated to some forms of human cancer, suggesting that these short molecules may represent a new class of genes involved in oncogenesis. In our study, we examined by microarray the global expression levels of 245 microRNAs in glioblastoma multiforme, the most frequent and malignant of primary brain tumors. The analysis of both glioblastoma tissues and glioblastoma cell lines allowed us to identify a group of microRNAs whose expression is significantly altered in this tumor. The most interesting results came from miR-221, strongly up-regulated in glioblastoma and from a set of brain-enriched miRNAs, miR-128, miR-181a, miR-181b, and miR-181c, which are down-regulated in glioblastoma.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Roma, Italy. ciafre@uniroma2.it
authors.forename: S A
authors.initials: SA
authors.lastname: Ciafrè
authors.forename: S
authors.initials: S
authors.lastname: Galardi
authors.forename: A
authors.initials: A
authors.lastname: Mangiola
authors.forename: M
authors.initials: M
authors.lastname: Ferracin
authors.forename: C-G
authors.initials: CG
authors.lastname: Liu
authors.forename: G
authors.initials: G
authors.lastname: Sabatino
authors.forename: M
authors.initials: M
authors.lastname: Negrini
authors.forename: G
authors.initials: G
authors.lastname: Maira
authors.forename: C M
authors.initials: CM
authors.lastname: Croce
authors.forename: M G
authors.initials: MG
authors.lastname: Farace
mesh_terms: D001921:Brain / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D012016:Reference Values
chemical_list: D035683:MicroRNAs