title: The transgenic Arabidopsis plant system, pER8-GFP, as a powerful tool in searching for natural product estrogen-agonists/antagonists.
abstract: The transgenic Arabidopsis plant system, pER8-GFP, may be used as a powerful tool in searching for natural estrogen-agonists/antagonists. Among selected plant extracts and natural products, the method was able to distinguish active extracts (e.g., Glycine max and Pueraria lobata) and pure compounds (e.g., 17beta-estradiol (1), genistein (10), and daidzein (11)) and also to distinguish effects of structural changes on activity. Thus, this rapid sensitive system was proven to be suitable for the discovery of natural products with estrogenic activity.
authors.affiliation: Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan, Republic of China. aaronfrc@kmu.edu.tw
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Chang
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Hayashi
authors.forename: Nam-Hai
authors.initials: NH
authors.lastname: Chua
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kamio
authors.forename: Zih-You
authors.initials: ZY
authors.lastname: Huang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nozaki
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
mesh_terms: D017360:Arabidopsis; D001685:Biological Factors / Q000302:isolation & purification* / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000302:isolation & purification*; D004967:Estrogens / Q000819:agonists* / Q000302:isolation & purification*; D048789:Phytoestrogens / Q000302:isolation & purification* / Q000494:pharmacology*; D030821:Plants, Genetically Modified; D013329:Structure-Activity Relationship
chemical_list: D001685:Biological Factors; D004965:Estrogen Antagonists; D004967:Estrogens; D048789:Phytoestrogens