Bone marrow engraftment analysis after granulocyte transfusion.
We present the case of a 6-year-old male who received an allogeneic bone marrow transplant as part of treatment for acute lymphoblastic leukemia. The patient relapsed 5 months after transplantation and received additional chemotherapy. He acquired an angioinvasive fungal infection that required transfusion of granulocytes. Approximately 5 weeks after relapsing (181 days after transplant), a bone marrow specimen was taken for molecular engraftment analysis and flow cytometry to assess graft loss as well as residual disease. The engraftment results generated by the multiple short tandem repeat loci tested were inconsistent, and alleles were present at several loci that were of neither patient nor donor origin. An error in specimen identification was initially considered. Further investigation into the circumstances surrounding procurement of the patient's bone marrow aspirate revealed that the patient had received a granulocyte transfusion approximately 10 hours before the bone marrow specimen was taken. In addition, morphological and flow cytometric analyses of the same bone marrow aspirate demonstrated a significant degree of peripheral blood contamination. We determined that the unknown alleles in the bone marrow engraftment specimen were derived from the donor of the transfused granulocytes. This case illustrates that white cell transfusion can lead to erroneous bone marrow engraftment results, particularly if only one microsatellite locus is used to monitor engraftment.
Swierczynski
Sharon L
SL
Department of Pathology, Johns Hopkins Medical Institutions, Park Building, Room SB202, 600 North Wolfe St., Baltimore, MD 21287, USA.
Hafez
Michael J
MJ
Philips
Juliet
J
Higman
Meghan A
MA
Berg
Karin D
KD
Murphy
Kathleen M
KM
D016026:Bone Marrow Transplantation; D002648:Child; D004247:DNA / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D006085:Graft Survival / Q000235:genetics*; D006098:Granulocytes / Q000637:transplantation; D006801:Humans; D017708:Leukocyte Transfusion; D008297:Male; D018895:Microsatellite Repeats; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000601:surgery; D014184:Transplantation, Homologous
D004247:DNA