Resolving overlap in two-dimensional NMR spectra: nuclear Overhauser effects in a polysaccharide.
Overlapping resonances in the two-dimensional nuclear Overhauser (NOESY) spectrum of the O-antigenic polysaccharide from Escherichia coli O147 have been resolved by recording a tilted projection of a three-dimensional NOESY-HSQC spectrum, where the carbon-13 and proton evolutions are linked together. Through the introduction of small contributions from the appropriate carbon-13 shifts, a cluster of five unresolved proton peaks is separated into its components without the need to perform the full three-dimensional measurement, a time saving of at least an order of magnitude.
Kupce
Eriks
E
Varian Ltd, Yarnton, Oxford, UK.
Nishida
Toshiaki
T
Widmalm
Göran
G
Freeman
Ray
R
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011522:Protons
D002247:Carbon Isotopes; D019081:O Antigens; D011522:Protons