title: Modified determination method of total bromine in agricultural products by gas chromatography.
abstract: In the official method for determination of total bromine in fruit and grain foods, bromine is derivatized with 3-pentanone for GC analysis. Co-existing substances sometimes interfere with measurement of the derivative, though the method is highly selective. In this study, the notification method was modified to reduce impurity peaks by applying 3-hexanone. Samples were alkalized and reduced to ash in an electric furnace. After ashing, samples were oxidized with potassium permanganate solution and derivatized with 3-hexanone. The calibration curve was linear from 0.1 microg/mL up to 5.0 microg/mL. The detection limit (S/N = 10) was 0.1 microg/mL, i.e., 5 microg/g for herb, 2.5 microg/g for grains and 1.0 microg/g for fruits. The recoveries of bromine from fruit, grain foods and herbs added at the levels of 5 to 25 microg/g ranged from 84.2 to 96.9%. The values of relative standard deviation (RSD) were from 1.4 to 6.3%. This method should be useful for routine examination of total bromine in foods.
authors.affiliation: Tokyo Metropolitan Institute of Public Health: 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Yamaki
authors.forename: Shozo
authors.initials: S
authors.lastname: Horii
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Takeo
authors.initials: T
authors.lastname: Sasamoto
authors.forename: Fusao
authors.initials: F
authors.lastname: Ushio
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Kamata
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ibe
mesh_terms: D001966:Bromine / Q000032:analysis*; D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D010422:Pentanones / Q000494:pharmacology
chemical_list: D010422:Pentanones; D001966:Bromine