Degradation of Chlamydia pneumoniae by peripheral blood monocytic cells.
Chlamydia pneumoniae is a common human respiratory pathogen that has been associated with a variety of chronic diseases, including atherosclerosis. The role of this organism in the pathogenesis of atherosclerosis remains unknown. A key question is how C. pneumoniae is transferred from the site of primary infection to a developing atherosclerotic plaque. It has been suggested that circulating monocytes could be vehicles for dissemination of C. pneumoniae since the organism has been detected in peripheral blood monocytic cells (PBMCs). In this study we focused on survival of C. pneumoniae within PBMCs isolated from the blood of healthy human donors. We found that C. pneumoniae does not grow and multiply in cultured primary monocytes. In C. pneumoniae-infected monocyte-derived macrophages, growth of the organism was very limited, and the majority of the bacteria were eradicated. We also found that the destruction of C. pneumoniae within infected macrophages resulted in a gradual diminution of chlamydial antigens, although some of these antigens could be detected for days after the initial infection. The detected antigens present in infected monocytes and monocyte-derived macrophages represented neither chlamydial inclusions nor intact organisms. The use of {N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]}-6-aminocaproyl-d-erythro-sphingosine as a vital stain for chlamydiae proved to be a sensitive method for identifying rare C. pneumoniae inclusions and was useful in the detection of even aberrant developmental forms.
Wolf
Katerina
K
Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, NIAID, NIH, Rocky Mountain Laboratories, Hamilton, MT 59840, USA.
Fischer
Elizabeth
E
Hackstadt
Ted
T
D000942:Antigens, Bacterial / Q000276:immunology; D015703:Antigens, CD / Q000378:metabolism; D016993:Chlamydophila pneumoniae / Q000276:immunology / Q000378:metabolism*; D022162:Cytoplasmic Vesicles / Q000382:microbiology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D002479:Inclusion Bodies / Q000276:immunology; D008070:Lipopolysaccharides / Q000378:metabolism; D051907:Lysosomal Membrane Proteins; D008264:Macrophages / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D009000:Monocytes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D010588:Phagosomes / Q000378:metabolism; D014176:Protein Biosynthesis / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology
D000942:Antigens, Bacterial; D015703:Antigens, CD; D008070:Lipopolysaccharides; D051907:Lysosomal Membrane Proteins