Recognition of host immune activation by Pseudomonas aeruginosa.
It is generally reasoned that lethal infections caused by opportunistic pathogens develop permissively by invading a host that is both physiologically stressed and immunologically compromised. However, an alternative hypothesis might be that opportunistic pathogens actively sense alterations in host immune function and respond by enhancing their virulence phenotype. We demonstrate that interferon-gamma binds to an outer membrane protein in Pseudomonas aeruginosa, OprF, resulting in the expression of a quorum-sensing dependent virulence determinant, the PA-I lectin. These observations provide details of the mechanisms by which prokaryotic organisms are directly signaled by immune activation in their eukaryotic host.
Wu
Licheng
L
Department of Surgery, University of Chicago, Pritzker School of Medicine, Chicago, IL 60637, USA.
Estrada
Oscar
O
Zaborina
Olga
O
Bains
Manjeet
M
Shen
Le
L
Kohler
Jonathan E
JE
Patel
Nachiket
N
Musch
Mark W
MW
Chang
Eugene B
EB
Fu
Yang-Xin
YX
Jacobs
Michael A
MA
Nishimura
Michael I
MI
Hancock
Robert E W
RE
Turner
Jerrold R
JR
Alverdy
John C
JC
D018829:Adhesins, Bacterial / Q000096:biosynthesis*; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D037102:Lectins / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D018272:Porins / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity*; D011710:Pyocyanine / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation; D014774:Virulence
D018829:Adhesins, Bacterial; D016207:Cytokines; D037102:Lectins; D018272:Porins; D011994:Recombinant Proteins; C038692:adhesin, Pseudomonas; D007371:Interferon-gamma; D011710:Pyocyanine