Urinary high performance reverse phase chromatography cortisol and cortisone analyses before and at the end of a race in elite cyclists.
A functional and basic method for the quantitative analysis of urine cortisol (F) and cortisone (E) using a Solid-Phase Extraction column and HPLC with ultraviolet detection is here described and validated to analyse urine samples. Urine specimens were analysed to study F and E relation and ratio in athletes and healthy sedentary subjects. The F and E concentrations in random urine specimens were significantly higher in the post exercise versus pre exercise condition in cyclists (F: 136+/-93 nmol/l versus 67+/-50 nmol/l (p<0.001); E: 797+/-400 nmol/l versus 408+/-252 nmol/l (p<0.001)). The F/E ratio was 0.18+/-0.11 versus 0.16+/-0.07, respectively, and a significant difference was only demonstrated comparing sedentary (0.11+/-0.07) and cyclist individuals at rest (p<0.05).
Gatti
Rosalba
R
Section of Clinical Biochemistry, Department of Medical Diagnostic Sciences and Spec. Ther., University of Padua, 35100 Padua, Italy.
Cappellin
Enrico
E
Zecchin
Barbara
B
Antonelli
Giorgia
G
Spinella
Paolo
P
Mantero
Franco
F
De Palo
Elio Franco
EF
D000293:Adolescent; D001642:Bicycling; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003348:Cortisone / Q000652:urine*; D006801:Humans; D006854:Hydrocortisone / Q000652:urine*; D008297:Male; D015203:Reproducibility of Results; D013997:Time Factors
D003348:Cortisone; D006854:Hydrocortisone