Photodynamic actions of indocyanine green and trypan blue on human lens epithelial cells in vitro.
OBJECTIVE
The purpose of this study was to evaluate the toxicity and photodynamic activity of indocyanine green (ICG) and trypan blue (TryB) on cultured human lensepithelial cells (LECs).


METHODS
Experimental study.


METHODS
Lens epithelial cell viability was assessed after treatment with ICG and TryB concentrations ranging from 0.025 to 5.0 mg/ml, and exposure to 806 nm diode laser.


RESULTS
At ICG concentrations below 0.5 mg/ml, there was > or =75% cell viability; at higher ICG concentrations there was dose-dependent cytotoxicity in addition to loss of cellular viability due to ICG photosensitization. TryB had little cytotoxicity to the LECs: >80% cells were viable irrespective of the dye concentration or laser treatment.


CONCLUSIONS
These data indicate that ICG may have application as a photosensitizer in the selective eradication of residual LECs after cataract surgery to reduce the incidence of posterior capsule opacification.
Melendez
Robert F
RF
Department of Ophthalmology, University of Texas Health Science Center, San Antonio, 78229, USA. robertmelendez@mac.com
Kumar
Neeru
N
Maswadi
Saher M
SM
Zaslow
Kenneth
K
Glickmank
Randolph D
RD
D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D007208:Indocyanine Green / Q000633:toxicity*; D007834:Lasers; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000633:toxicity*; D014343:Trypan Blue / Q000633:toxicity*
D004396:Coloring Agents; D017319:Photosensitizing Agents; D014343:Trypan Blue; D007208:Indocyanine Green