title: Validation of basophil histamine release against the autologous serum skin test and outcome of serum-induced basophil histamine release studies in a large population of chronic urticaria patients.
abstract: BACKGROUND
  Endogenous histamine-releasing factors (HRFs) are involved in 30-60% of patients with chronic urticaria (CU). Evidence for their existence comes from in vivo studies of autoreactivity with the autologous serum skin test (ASST), in vitro immunoassays demonstrating autoantibodies against the immunoglobulin E (IgE) or the high affinity IgE receptor (FcepsilonRI) and serum-induced histamine release (HR) from basophils and mast cells. We have examined the correlation between the ASST and a new basophil histamine-releasing assay (the HR-Urtikaria test) in a group of well-characterized CU patients and subsequently determined the frequency of HR-Urticaria-positive sera from a larger population of CU patients.
  
  
  METHODS
  Group 1 consisted of 28 patients with CU (16 were ASST-positive) 20 patients with atopic dermatitis, 24 patients with allergy to birch and nine healthy controls. Group 2 consisted of 873 unselected CU patients.
  
  
  METHODS
  White blood cells containing 1-2% basophils from a healthy nonatopic donor were incubated with patients sera in the presence of interleukin (IL)-3. Histamine was measured by the glass fibre method.
  
  
  RESULTS
  Using the ASST as the true outcome, the HR-Urticaria test showed a sensitivity and specificity of 75% in group 1 using a cut-off value for HR of >16.5%. None of the controls was positive in the HR-Urticaria test. In group 2, we found no difference in the frequency of positives between male (34.6%, n = 254) and female adults (35.1%, n = 576) but twice as many females as males were tested.
  
  
  CONCLUSIONS
  Our studies have shown that the HR-Urticaria test has a good sensitivity and specificity for endogenous HRFs demonstrated by the ASST in patients with CU and that about one-third of unselected patients with CU have a positive result.
authors.affiliation: National University Hospital, Allergy Clinic, Laboratory of Medical Allergology, Copenhagen, Denmark.
authors.forename: M H
authors.initials: MH
authors.lastname: Platzer
authors.forename: C E H
authors.initials: CE
authors.lastname: Grattan
authors.forename: L K
authors.initials: LK
authors.lastname: Poulsen
authors.forename: P S
authors.initials: PS
authors.lastname: Skov
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000276:immunology; D001491:Basophils / Q000276:immunology / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D005260:Female; D006632:Histamine / Q000096:biosynthesis*; D006801:Humans; D007118:Immunoassay; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012882:Skin Tests; D014581:Urticaria / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D006632:Histamine