Effect of oxidized-LDL on NF-kappaB nuclear translocation in aortic smooth muscle cells originated from rats of different ages.
OBJECTIVE
To investigate the molecular mechanism of atherosclerosis that related to age.


METHODS
Immunohistochemistry staining and Western blot were adopted to determine the nuclear translocation of nuclear factor-kappa B (NF-kappaB) and expression of platelet-derived growth factor B (PDGF-B) in smooth muscle cells (SMCs) co-cultured with low density lipoprotein (LDL), oxidized LDL (ox-LDL), and ox-LDL+high density lipoprotein (HDL) originated from rats of 2 and 10 months old respectively. Fat stain was used to identify the lipid intake in SMCs.


RESULTS
The optimal stimulation time of ox-LDL to SMCs was 12 hours. NF-kappaB intensity increased in most nuclei of SMCs that originated from rats of either 2 or 10 months old co-cultured with ox-LDL. The intensity of NF-KB and the amount of intracellular lipid taken in SMCs were more obvious in cells from 10-month-old rats than from the younger ones. Change of PDGF-B expression in SMCs was not remarkable in each group of rats.


CONCLUSIONS
The 10-month-old rats are more susceptive to ox-LDL than 2-month-old rats in activating nuclear translocation of NF-kappaB. Maybe this is one of the important reasons contributing to the difference between the older and younger rats on the initiation and development of atherosclerosis lesion. Expression of PDGF-B is not associated with the activity of nuclear translocation of NF-kappaB.
Zhang
Jun-hua
JH
Department of Pathology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing.
Zhou
Li
L
Yin
Hong-chao
HC
Liu
Pei-mao
PM
Zhang
Hua
H
She
Ming-peng
MP
D000367:Age Factors; D000818:Animals; D001011:Aorta / Q000166:cytology; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D008075:Lipoproteins, HDL / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D003470:Culture Media; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D016328:NF-kappa B; D020574:Proto-Oncogene Proteins c-sis; C099252:oxidized low density lipoprotein