title: Stage-dependent gene expression profiles during natural killer cell development.
abstract: Natural killer (NK) cells develop from hematopoietic stem cells (HSCs) in the bone marrow. To understand the molecular regulation of NK cell development, serial analysis of gene expression (SAGE) was applied to HSCs, NK precursor (pNK) cells, and mature NK cells (mNK) cultured without or with OP9 stromal cells. From 170,464 total individual tags from four SAGE libraries, 35,385 unique genes were identified. A set of genes was expressed in a stage-specific manner: 15 genes in HSCs, 30 genes in pNK cells, and 27 genes in mNK cells. Among them, lipoprotein lipase induced NK cell maturation and cytotoxic activity. Identification of genome-wide profiles of gene expression in different stages of NK cell development affords us a fundamental basis for defining the molecular network during NK cell development.
authors.affiliation: Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Hyung-Sik
authors.initials: HS
authors.lastname: Kang
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Kim
authors.forename: Sanggyu
authors.initials: S
authors.lastname: Lee
authors.forename: Suk-Ran
authors.initials: SR
authors.lastname: Yoon
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Kawamura
authors.forename: Young-Cheol
authors.initials: YC
authors.lastname: Lee
authors.forename: Sangsoo
authors.initials: S
authors.lastname: Kim
authors.forename: Pyung-Keun
authors.initials: PK
authors.lastname: Myung
authors.forename: San Ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Inpyo
authors.initials: I
authors.lastname: Choi
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D020869:Gene Expression Profiling; D015723:Gene Library; D006412:Hematopoietic Stem Cells / Q000502:physiology; D007694:Killer Cells, Natural / Q000502:physiology*; D008071:Lipoprotein Lipase / Q000096:biosynthesis / Q000235:genetics; D051379:Mice
chemical_list: D008071:Lipoprotein Lipase