Sensitive MS/MS-liquid chromatography assay for simultaneous determination of tegafur, 5-fluorouracil and 5-fluorodihydrouracil in plasma.
Ftorafur (FT), an oral prodrug of 5-FU, is part of UFT and S1, two oral prodrugs widely used in digestive tract cancer. We set up a liquid chromatography tandem mass spectrometry (LC/MS-MS) method, chosen for its specificity of detection, for simultaneously measuring in human plasma FT, 5-FU and 5-FUH2. Separation was performed on a Hypercarb column. Linearity, precision and accuracy were validated in the concentration range studied for each compound. This simple and reliable LC/MS-MS method allows specific, sensitive and reproducible quantification of FT, 5-FU and FUH2 in human plasma and can be applied to further pharmacokinetic studies in patients treated with FT-based prodrugs.
Remaud
G
G
Oncopharmacology-Pharmacogenetic, INSERM U564, Anticancer Centre Paul Papin, 2 rue Moll, 49933 Angers Cedex 9, France.
Boisdron-Celle
M
M
Morel
A
A
Gamelin
A
A
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005472:Fluorouracil / Q000031:analogs & derivatives / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D005641:Tegafur / Q000097:blood; D013997:Time Factors
D005641:Tegafur; C019389:5-fluorodihydrouracil; D005472:Fluorouracil