Pesticide resistance via transposition-mediated adaptive gene truncation in Drosophila.
To study adaptation, it is essential to identify multiple adaptive mutations and to characterize their molecular, phenotypic, selective, and ecological consequences. Here we describe a genomic screen for adaptive insertions of transposable elements in Drosophila. Using a pilot application of this screen, we have identified an adaptive transposable element insertion, which truncates a gene and apparently generates a functional protein in the process. The insertion of this transposable element confers increased resistance to an organophosphate pesticide and has spread in D. melanogaster recently.
Aminetzach
Yael T
YT
Department of Biological Sciences, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA.
Macpherson
J Michael
JM
Petrov
Dmitri A
DA
D000222:Adaptation, Physiological; D000483:Alleles; D019943:Amino Acid Substitution; D000818:Animals; D001387:Azinphosmethyl / Q000494:pharmacology; D001483:Base Sequence; D002794:Choline / Q000378:metabolism; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004330:Drosophila / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D019143:Evolution, Molecular; D005091:Exons; D005260:Female; D015870:Gene Expression; D017344:Genes, Insect; D006239:Haplotypes; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology; D007438:Introns; D020084:Long Interspersed Nucleotide Elements; D008969:Molecular Sequence Data; D009154:Mutation; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D012641:Selection, Genetic
D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D007306:Insecticides; D001387:Azinphosmethyl; D002794:Choline