title: Cytotoxic effect of 7beta-hydroxycholesterol on human NCI-H460 lung cancer cells.
abstract: The cytotoxic activity of 7beta-hydroxycholesterol (7beta-OHC) was evaluated on human NCI-H460 lung cancer cells. 7beta-OHC decreased clonogenic survival of NCI-H460 in a dose dependent pattern. 7beta-OHC induced apoptosis in NCI-H460, with the characteristic features like increase in sub-G(1) hypodiploid (apoptotic) cells, and apoptotic body formation, as evidenced by flow cytometry and fluorescence microscope, respectively. Apoptosis was also associated with loss of mitochondrial transmembrane potential, and the activation of caspases 9 and 3. 7beta-OHC resulted in generation of reactive oxygen species (ROS) during apoptosis. On the whole, the results indicated that 7beta-OHC inhibited the proliferation of NCI-H460 cells through apoptosis via caspase activation.
authors.affiliation: Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Korea.
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.forename: Sungwook
authors.initials: S
authors.lastname: Chae
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Lee
authors.forename: Moon Taek
authors.initials: MT
authors.lastname: Park
authors.forename: Su Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Yun Sil
authors.initials: YS
authors.lastname: Lee
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000473:pathology*; D008564:Membrane Potentials; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D017382:Reactive Oxygen Species
chemical_list: D006888:Hydroxycholesterols; D017382:Reactive Oxygen Species; C011724:cholest-5-en-3 beta,7 alpha-diol