Role of parasympathetic overactivity in water immersion stress-induced gastric mucosal lesion in rat.
Stress ulcer is clinically prevalent, but the underlying mechanisms are not well understood. This study aimed to investigate the role of sympathovagal imbalance in the development of water immersion restraint stress (WRS)-induced gastric mucosal lesion. Wistar rats were subjected to either restraint stress (RS) (n = 7) or WRS (n = 7) for 5 h. Linear parameters of heart rate variability and Poincaré plot were analyzed on the basis of the surface ECGs. Gastric mucosal lesion was evaluated by gross anatomy and histology. Mean R-R intervals significantly increased (P < 0.001) in a time-dependent manner in the WRS group but slightly decreased (P < 0.001) in the RS group. Root mean square of successive differences of R-R intervals and high-frequency norm (high-frequency power normalized by the total frequency power) were significantly higher in the WRS group than the RS group (P < 0.001). Low-frequency norm and low-to-high-frequency ratio increased significantly 1 h after stress and then declined to similar levels in both groups. The Poincaré plots of R-R intervals in the WRS group shifted right-upwardly and showed dispersed patterns compared with the RS group. Gastric mucosae showed serious hemorrhage, effusion, and structural collapse in the WRS group but remained normal in the RS group. Bilateral cervical vagotomy suppressed the increase of heart rate variability and prevented the gastric mucosal lesion induced by WRS. We conclude that parasympathetic overactivity is the predominant autonomic response to WRS and is most probably the leading mechanism of WRS-induced gastric mucosal lesion in rat.
Xie
Yuan-Fang
YF
Dept. of Biomedical Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Jiao
Qing
Q
Guo
Shu
S
Wang
Fu-Zhen
FZ
Cao
Ji-Min
JM
Zhang
Zheng-Guo
ZG
D000818:Animals; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000294:innervation* / Q000473:pathology / Q000503:physiopathology*; D006321:Heart / Q000294:innervation / Q000503:physiopathology; D006339:Heart Rate; D007101:Immersion / Q000009:adverse effects* / Q000503:physiopathology*; D008297:Male; D010275:Parasympathetic Nervous System / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000294:innervation* / Q000503:physiopathology*; D013312:Stress, Physiological / Q000209:etiology / Q000473:pathology / Q000503:physiopathology