title: Agaric acid induces mitochondrial permeability transition through its interaction with the adenine nucleotide translocase. Its dependence on membrane fluidity.
abstract: The effect of agaric acid as inducer of mitochondrial permeability transition was studied. It was found that: (i) agaric acid (AA) promoted efflux of accumulated Ca2+, collapse of transmembrane potential, and mitochondrial swelling; (ii) these effects depend on membrane fluidity; (iii) ADP inhibited the effect of AA on Ca2+ efflux, and (iv) AA blocked binding of the sulfhydryl reagent, eosin-5-maleimide, to the adenine nucleotide translocase. It is proposed that AA induces pore opening through binding of the citrate moiety to the ADP/ATP carrier; this interaction must be stabilized by insertion of the alkyl chain in the lipid milieu of the membrane.
authors.affiliation: Departamento de Bioquímica, Instituto Nacional de Cardiología, Ignacio Chávez, Juan Badiano No. 1, Col. Sección XVI, Tlalpan, DF 014080, Mexico.
authors.forename: Noemí
authors.initials: N
authors.lastname: García
authors.forename: Cecilia
authors.initials: C
authors.lastname: Zazueta
authors.forename: Natalia
authors.initials: N
authors.lastname: Pavón
authors.forename: Edmundo
authors.initials: E
authors.lastname: Chávez
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D019343:Citric Acid / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004801:Eosine Yellowish-(YS) / Q000031:analogs & derivatives / Q000378:metabolism; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology*; D007653:Ketocholesterols / Q000494:pharmacology; D008560:Membrane Fluidity / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D000226:Mitochondrial ADP, ATP Translocases / Q000187:drug effects* / Q000378:metabolism; D008933:Mitochondrial Swelling / Q000187:drug effects; D051381:Rats; D013439:Sulfhydryl Reagents / Q000378:metabolism; D013696:Temperature
chemical_list: D007653:Ketocholesterols; D013439:Sulfhydryl Reagents; D019343:Citric Acid; C063833:6-ketocholestanol; C011396:agaric acid; D000244:Adenosine Diphosphate; C033531:eosin maleimide; D000226:Mitochondrial ADP, ATP Translocases; D002118:Calcium; D004801:Eosine Yellowish-(YS)