Antiangiogenic activities of polysaccharides isolated from medicinal fungi.
Extracted polysaccharides from medicinal fungi, including Antrodia cinnamomea, Antrodia malicola, Antrodia xantha, Antrodiella liebmannii, Agaricus murrill, and Rigidoporus ulmarius, were investigated for their effects on vascular endothelial growth factor (VEGF)-induced tube formation in endothelial cells (ECs). Chemical analysis revealed that myo-inositol, sorbitol, fucose, galactosamine, glucosamine, galactose, glucose, and mannose were the neutral sugars in these polysaccharides. These fungal polysaccharides showed no toxicity to ECs. For the inhibition of endothelial tube formation, extracted polysaccharides from A. xantha and R. ulmarius were shown to produce greater inhibition compared to those from other fungi. Fucose, glucose and mannose were the predominant monosaccharides from these two fungi. These results suggest that monosaccharides may play a role in the inhibitory effect of these fungi on endothelial tube formation. In contrast to the inhibition on tube formation from polysaccharides of A. cinnamomea and A. malicola, polysaccharides from A. xantha and R. ulmarius, with molecular weight between 2693-2876 and 304-325 kDa, were critical for this inhibitory activity. Our results show that polysaccharides isolated from A. xantha and R. ulmarius provide greater antiangiogenesis than those from commercialized A. murrill (Brazilian mushroom) and A. cinnamomea. These studies provide a basis for the potential development of these polysaccharides for antiangiogenesis usage.
Chen
Shih Chung
SC
Department of Medicine, Taipei Medical University-Wan-Fang Hospital, Taiwan; Institute of Clinical Medicine, National Yang-Ming University Medical College, Taipei 116, Taiwan.
Lu
Mei-Kuang
MK
Cheng
Jing-Jy
JJ
Wang
Danny Ling
DL
D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D001011:Aorta; D001487:Basidiomycota / Q000737:chemistry*; D002417:Cattle; D002850:Chromatography, Gel; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology
D020533:Angiogenesis Inhibitors; D009005:Monosaccharides; D011134:Polysaccharides