High-level production of arachidonic acid by fed-batch culture of Mortierella alpina using NH4OH as a nitrogen source and pH control.
Mortierella alpina was grown in a fed-batch culture using a 12-l jar fermenter with an initial 8-l working volume containing 20 g glucose l-1 and 10 g corn-steep powder l-1. Glucose was intermittently fed to give 32 g l-1 at each time. The pH of culture was maintained using 14% (v/v) NH4OH, which also acted as a nitrogen source. A final cell density of 72.5 g l-1 was reached after 12.5 days with a content of arachidonic acid (ARA) at 18.8 g l-1. These values were 4 and 1.8 times higher than the respective values in batch culture. Our results suggest that the combined feeding of glucose and NH4+ to the growth of M. alpina could be applied for the industrial scale production of ARA.
Hwang
Byung-Hae
BH
Department of Molecular Science and Technology, Ajou University, San5, Woncheong-dong, 442-749, Yeongtong-gu, Suwon, Republic of Korea.
Kim
Jin-Wook
JW
Park
Chang-Yeol
CY
Park
Chang-Seo
CS
Kim
Yong-Sung
YS
Ryu
Yeon-Woo
YW
D064753:Ammonium Hydroxide; D016718:Arachidonic Acid / Q000096:biosynthesis*; D019149:Bioreactors / Q000382:microbiology; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000378:metabolism* / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis; D020094:Mortierella / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D025282:Mycelium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism* / Q000494:pharmacology; D013997:Time Factors
D003470:Culture Media; D006878:Hydroxides; D008055:Lipids; D000644:Quaternary Ammonium Compounds; D016718:Arachidonic Acid; D064753:Ammonium Hydroxide; D005947:Glucose