Involvement of a small GTP binding protein in HIV-1 release.
BACKGROUND
There is evidence suggesting that actin binding to HIV-1 encoded proteins, or even actin dynamics themselves, might play a key role in virus budding and/or release from the infected cell. A crucial step in the reorganisation of the actin cytoskeleton is the engagement of various different GTP binding proteins. We have thus studied the involvement of GTP-binding proteins in the final steps of the HIV-1 viral replication cycle.


RESULTS
Our results demonstrate that virus production is abolished when cellular GTP binding proteins involved in actin polymerisation are inhibited with specific toxins.


CONCLUSIONS
We propose a new HIV budding working model whereby Gag interactions with pre-existing endosomal cellular tracks as well as with a yet non identified element of the actin polymerisation pathway are required in order to allow HIV-1 to be released from the infected cell.
Audoly
Gilles
G
Unité des Rickettsies, CNRS UMR6020, Faculté de Médecine, 27 bd Jean Moulin, 13385 Marseille cedex 05, IFR48, France. gilles.audoly@medecine.univ-mrs.fr
Popoff
Michel R
MR
Gluschankof
Pablo
P
D036002:ADP Ribose Transferases / Q000494:pharmacology; D001426:Bacterial Proteins / Q000494:pharmacology; D001427:Bacterial Toxins / Q000494:pharmacology; D001905:Botulinum Toxins / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000502:physiology*; D015683:Gene Products, gag / Q000378:metabolism; D016655:HIV Core Protein p24 / Q000378:metabolism; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D011498:Protein Precursors / Q000378:metabolism; D020764:cdc42 GTP-Binding Protein / Q000502:physiology
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D015683:Gene Products, gag; D016655:HIV Core Protein p24; D011498:Protein Precursors; C072292:lethal toxin LT, Clostridium sordellii; C059767:p55 gag precursor protein, Human immunodeficiency virus 1; C031815:toxB protein, Clostridium difficile; D015638:Cytochalasin D; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins; D019204:GTP-Binding Proteins; D020764:cdc42 GTP-Binding Protein