title: Cytotoxicities of xanthones and cinnamate esters from Hypericum hookerianum.
abstract: 5-Hydroxy-2-methoxyxanthone (1), 2-hydroxy-3-methoxyxanthone (2), trans-kielcorin (3), 4-hydroxy-3-methoxyphenyl ferulate (4) and 3beta-O-caffeoylbetulinic acid (5) were isolated from Hypericum hookerianum. Compounds 1-5 were tested against the growth of three human tumor cell lines, MCF-7, NCI-H460 and SF-268. Compounds 4 and 5 exhibited significant inhibitory activity effects against all three; GI50 values for 4 were 15.1 +/- 1.6, 18.7 +/- 2.3 and 15.9 +/- 2.7 and for 5 12.2 +/- 2.4, 19.6 +/- 2.3 and 24.3 +/- 2.5. Compound 3 was less active with GI50 values of 55.1 +/- 2.3, 49.7 +/- 3.0 and 40.5 +/- 1.5, while 1 and 2 exhibited only weak effects. Compounds 4 and 5 were moderately effective in influencing the mitogenic response to human lymphocytes to hemoagglutinin, with IC values of 26.1 +/- 3.6 and 40.8 +/- 4.9, respectively.
authors.affiliation: ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Porto, Portugal.
authors.forename: Rujida
authors.initials: R
authors.lastname: Wilairat
authors.forename: Jiradej
authors.initials: J
authors.lastname: Manosroi
authors.forename: Aranya
authors.initials: A
authors.lastname: Manosroi
authors.forename: Anake
authors.initials: A
authors.lastname: Kijjoa
authors.forename: Maria São
authors.initials: MS
authors.lastname: Nascimento
authors.forename: Madalena
authors.initials: M
authors.lastname: Pinto
authors.forename: Artur M
authors.initials: AM
authors.lastname: Silva
authors.forename: Graham
authors.initials: G
authors.lastname: Eaton
authors.forename: Werner
authors.initials: W
authors.lastname: Herz
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002934:Cinnamates / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D004952:Esters / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D020902:Hypericum; D020128:Inhibitory Concentration 50; D008214:Lymphocytes / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D044004:Xanthones / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002934:Cinnamates; D004952:Esters; D010936:Plant Extracts; D044004:Xanthones