Allosterically activated Diels-Alder catalysis by a ribozyme.
We describe the allosteric control of Diels-Alder reactions by a small organic effector, theophylline. This is achieved by converting a Diels-Alder ribozyme into an allosterically regulated system. In contrast to other published systems, we have a bond-forming reaction with two small-molecule substrates and multiple turnover. This system could be very attractive for the development of assays for a variety of analytes and can be regarded as a prototype of fully synthetic signaling cascades.
Helm
Mark
M
Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
Petermeier
Markus
M
Ge
Bixia
B
Fiammengo
Roberto
R
Jäschke
Andres
A
D000873:Anthracenes / Q000737:chemistry*; D001483:Base Sequence; D001710:Biotin / Q000737:chemistry; D002384:Catalysis; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry; D007700:Kinetics; D008301:Maleimides / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D013806:Theophylline / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry
D000873:Anthracenes; D008301:Maleimides; D009711:Nucleotides; D016337:RNA, Catalytic; D014764:Viral Proteins; D012313:RNA; D001710:Biotin; D013806:Theophylline; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; C034020:anthracene