title: Homologous prostatic fluid added to frozen-thawed dog spermatozoa prior to intravaginal insemination of bitches resulted in better fertility than albumin-free TALP.
abstract: The aim of this study was to determine whether canine prostatic fluid has intrinsic effects resulting in higher fertility than albumin-free Tyrode's albumin lactate pyruvate (afTALP) when added to thawed semen prior to intravaginal insemination. Twenty-four German shepherd bitches were inseminated intravaginally with frozen-thawed spermatozoa to which either homologous prostatic fluid (Group P; 12 bitches) or afTALP (Group T; 12 bitches) was added to give a final insemination volume of 7mL. Each bitch was inseminated daily starting when the vaginal folds first became angular and continuing until the day before a diestrus vaginal smear was first seen. Bitches were spayed about 3 weeks after the onset of diestrus and the number of corpora lutea and the number of conceptuses counted. Group P and Group T bitches were, respectively, inseminated 5.3+/-1.0 and 5.8+/-2.1 times with 48.9+/-8.6 and 50.4+/-8.3 million progressively motile spermatozoa per insemination. Eight Group P bitches and 10 Group T bitches conceived with totals of 76 and 45 conceptuses and 126 and 117 corpora lutea, respectively. Odds of conception were taken as the number of conceptuses divided by (the number of corpora lutea minus the number of conceptuses). After adjustment for the number of progressively motile spermatozoa per day and the random effect of bitch, the addition of prostatic fluid resulted in an increased odds of conception compared to afTALP. This effect decreased as the number of progressively motile spermatozoa per day increased.
authors.affiliation: Section of Reproduction, Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. johan.nothling@up.ac.za
authors.forename: J O
authors.initials: JO
authors.lastname: Nöthling
authors.forename: R
authors.initials: R
authors.lastname: Shuttleworth
authors.forename: K
authors.initials: K
authors.lastname: de Haas
authors.forename: P N
authors.initials: PN
authors.lastname: Thompson
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary; D004285:Dogs; D005260:Female; D005298:Fertility; D006358:Hot Temperature; D007315:Insemination, Artificial / Q000662:veterinary*; D007552:Isotonic Solutions; D008297:Male; D011467:Prostate / Q000502:physiology*; D012044:Regression Analysis; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D007552:Isotonic Solutions; C037692:Tyrode's solution