A novel type of immunity protein, NukH, for the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1.
Staphylococcus warneri ISK-1 produces a lantibiotic, nukacin ISK-1. The nukacin ISK-1 gene cluster consists of at least six genes, nukA, -M, -T, -F, -E, and -G, and two open reading frames, ORF1 and ORF7 (designated nukH). Sequence comparisons suggested that NukF, -E, -G, and -H contribute to immunity to nukacin ISK-1. We investigated the immunity levels of recombinant Lactococcus lactis expressing nukFEG and nukH against nukacin ISK-1. The co-expression of nukFEG and nukH resulted in a high degree of immunity. The expression of either nukFEG or nukH conferred partial immunity against nukacin ISK-1. These results suggest that NukH contributes cooperatively to self-protection with NukFEG. The nukacin ISK-1 immunity system might function against another lantibiotic, lacticin 481. Western blot analysis showed that NukH expressed in Staphylococcus carnosus was localized in the membrane. Peptide release/bind assays indicated that the recombinant L. lactis expressing nukH interacted with nukacin ISK-1 and lacticin 481 but not with nisin A. These findings suggest that NukH contributes cooperatively to host immunity as a novel type of lantibiotic-binding immunity protein with NukFEG.
Aso
Yuji
Y
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University.
Okuda
Ken-ichi
K
Nagao
Jun-ichi
J
Kanemasa
Youhei
Y
Thi Bich Phuong
Nguyen
N
Koga
Hanako
H
Shioya
Kouki
K
Sashihara
Toshihiro
T
Nakayama
Jiro
J
Sonomoto
Kenji
K
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001430:Bacteriocins / Q000276:immunology / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family / Q000235:genetics; D009561:Nisin / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D013210:Staphylococcus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D001426:Bacterial Proteins; D001430:Bacteriocins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C421186:nukacin ISK-1; C082508:lacticin 481; D009561:Nisin; C102526:nisin A