Identification of three isoforms for mitochondrial adenine nucleotide translocator in the pufferfish Takifugu rubripes.
Three adenine nucleotide translocator (ANT) genes were identified through in silico data mining of the Fugu genome database along with isolation of their corresponding cDNAs in vivo from the pufferfish (Takifugu rubripes). As a result of phylogenetic analysis, the ANT gene on scaffold_254 corresponded to mammalian ANT1, whereas both of those on scaffold_6 and scaffold_598 to mammalian ANT3. The ANT gene encoded by scaffold_6 was expressed ubiquitously in various tissues, whereas the ANT genes encoded by scaffold_254 and scaffold_598 were predominantly expressed in skeletal muscle and heart, respectively.
Itoi
Shiro
S
Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.
Misaki
Ryohei
R
Hirayama
Makoto
M
Nakaniwa
Makiko
M
Liang
Chun-Shi
CS
Kondo
Hidehiro
H
Watabe
Shugo
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D017124:Conserved Sequence; D018076:DNA, Complementary; D016678:Genome; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D008929:Mitochondria, Heart / Q000201:enzymology; D008931:Mitochondria, Muscle / Q000201:enzymology; D000226:Mitochondrial ADP, ATP Translocases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D023741:Takifugu / Q000235:genetics*; D014018:Tissue Distribution; D014158:Transcription, Genetic
D018076:DNA, Complementary; D007527:Isoenzymes; D000226:Mitochondrial ADP, ATP Translocases