Synthesis of linear, branched, and cyclic peptide chimera.
Chimeric peptides are unnatural constructs consisting of bioactive compounds from at least two different peptide(s) and/or protein(s) or two sequences from different parts of the same protein. Such multifunctional peptide combinations are prepared to enhance the biological activity or selectivity of their components. New biological effects can also be achieved with the chimera. In this chapter the synthesis of three different types of chimeric peptides will be described. In a linear chimera, two peptide epitopes from different parts of glycoprotein D (gD) of herpes simplex virus (HSV) are combined. A branched chimera, built from linear peptides, consists of tuftsin oligomers with immunostimulatory activity and an epitope peptide of HSV gD. The third compound is a cyclic chimeric molecule, where alpha-conotoxin GI as a host peptide is modified by the incorporation of a core epitope from HSV gD as a guest sequence.
Mezö
Gábor
G
Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, Budapest, Hungary.
Hudecz
Ferenc
F
D000595:Amino Acid Sequence; D000818:Animals; D020916:Conotoxins / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics; D014405:Tuftsin / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D014759:Viral Envelope Proteins / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics
D020916:Conotoxins; D010456:Peptides, Cyclic; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1; C029960:conotoxin GI; D014405:Tuftsin