Reinnervation of muscular targets by nerve regeneration through guidance conduits.
We established histopathologic and neurophysiologic approaches to examine whether different designs of polycaprolactone-engineered nerve conduits (hollow vs. laminated) could promote nerve regeneration as autologous grafts after transection of sciatic nerves. The assessments included morphometric analysis at the level of sciatic nerve, neuromuscular junction (NMJ) and gastrocnemius muscle, and nerve conduction studies on sciatic nerves. Six months after nerve grafting, the nerve fiber density in the hollow-conduit group was similar to that in the autologous-graft group; the laminated-conduit group only achieved approximately 20% of these values. The consequences of these differences were reflected in nerve growth into muscular targets; this was demonstrated by combined cholinesterase histochemistry for NMJ and immunohistochemistry for nerve fibers innervating NMJ with an axonal marker, protein gene product 9.5. Hollow conduits had similar index of NMJ innervation as autologous grafts; the values were higher than those of laminated conduits. Among the 3 groups there were same patterns of differences in the cross-sectional area of muscle fibers and amplitudes of compound muscle action potential. These results indicate that hollow conduits were as efficient as autologous grafts to facilitate nerve regeneration, and provide a multidisciplinary approach to quantitatively evaluate muscular reinnervation after nerve injury.
Chiang
Hou-Yu
HY
Department of Anatomy and Cell Biology, National Taiwan University College of Medicine, Taipei, Taiwan.
Chien
Hsiung-Fei
HF
Shen
Hsin-Hsin
HH
Yang
Jean-Dean
JD
Chen
Yu-Hua
YH
Chen
Jui-Hsiang
JH
Hsieh
Sung-Tsang
ST
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001672:Biocompatible Materials; D002802:Cholinesterases / Q000378:metabolism; D004594:Electrophysiology; D007150:Immunohistochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D018482:Muscle, Skeletal / Q000294:innervation*; D009416:Nerve Regeneration / Q000502:physiology*; D009469:Neuromuscular Junction / Q000502:physiology; D011091:Polyesters; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology* / Q000637:transplantation / Q000648:ultrastructure; D014184:Transplantation, Homologous
D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D002802:Cholinesterases