Enhanced production of Penicillium expansum PED-03 lipase through control of culture conditions and application of the crude enzyme in kinetic resolution of racemic Allethrolone.
Alkaline lipase production was performed in submerged fermentation by Penicillium expansum PED-03. It was found that the suitable carbon source and nitrogen source for lipase production were 0.5% starch and 4.0% soybean meal, respectively. The maximal lipase activity (850 U/mL) of production was achieved at initial pH 5.5-6.0, 26 degrees C, 72 h. Tween-80 was an effective enhancer for lipase production. Agitation speed of the fermentor played an important role, and the suitable agitation speed for lipase production was 500 r/min. The lipase was stable within the range of pH 7.0-10.0 and 20-40 degrees C, and the optimum conditions for the enzymatic reaction were 35 degrees C and pH 9.5. The enzymatic resolution of racemic allethrolone (4-hydroxy-3-methyl-2-(2-propenyl)-2- cyclopenten-1-one) was carried out by the lipase from P. expansum PED-03, and the conversion reached 48% with excellent enantioselectivity (E > 100), which showed a good application potential in the production of optically pure allethrolone.
Dai
Dazhang
D
Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, P. R. China.
Xia
Liming
L
D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D003517:Cyclopentanes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D007536:Isomerism; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism*; D013025:Glycine max; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature
D003470:Culture Media; D003517:Cyclopentanes; D013501:Surface-Active Agents; C506804:allethrolone; D002244:Carbon; D008049:Lipase