Histochemical and immunohistochemical evaluation of gingival collagen and metalloproteinases in peri-implantitis.
The extra-cellular matrix of the gingival tissue plays an important role in the homeostasis of dental implants. In this work we have studied immunohistochemically the distribution of collagen I-III-IV-V, tenascin, metalloproteinases (MMP) 1-3-8-13 and TIMP-1 in three groups of patients: (1) subjects with natural teeth (healthy periodontal tissue), (2) subjects with normal peri-implant mucosa and (3) subjects with clinically evident peri-implantitis. The immunolabelling for collagen I-III-IV showed a similar pattern in all three groups. The labelling for collagen V increased in lamina propria of healthy peri-implant tissue and peri-implantitis. Tenascin immunolabelling in healthy and peri-implant tissues was scattered in lamina propria. In peri-implantitis tenascin immunolabelling increased mainly near to the basal lamina. The MMP-1-3-8 and TIMP-1 immunolabelling were very faint and localized in the stroma in all three groups. In healthy and peri-implant tissues MMP-13 immunolabelling was found in the lamina propria whereas in peri-implantitis MMP-13 immunolabelling was also in epithelium. On the whole, these data suggest that in the extracellular matrix of peri-implantitis there are alterations of collagen V, tenascin and MMP-13 patterns.
Borsani
Elisa
E
Division of Human Anatomy, Department of Biomedical Sciences and Biotechnologies, University of Brescia, Viale Europa, 11, 25123 Italy.
Salgarello
Stefano
S
Mensi
Magda
M
Boninsegna
Ramon
R
Stacchiotti
Alessandra
A
Rezzani
Rita
R
Sapelli
Pierluigi
P
Bianchi
Rossella
R
Rodella
Luigi F
LF
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D015921:Dental Implants; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005881:Gingiva / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D045726:Metalloproteases / Q000378:metabolism*; D008875:Middle Aged; D010518:Periodontitis / Q000378:metabolism*; D013194:Staining and Labeling; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
D015415:Biomarkers; D015921:Dental Implants; D019715:Tissue Inhibitor of Metalloproteinase-1; D003094:Collagen; D045726:Metalloproteases