A novel strategy using MASCOT Distiller for analysis of cleavable isotope-coded affinity tag data to quantify protein changes in plasma.
A novel strategy consisting of cleavable Isotope-Coded Affinity Tag (cICAT) combined with MASCOT Distiller was evaluated as a tool for the quantification of proteins in "abnormal" patient plasma, prepared by pooling samples from patients with acute stroke. Quantification of all light and heavy cICAT-labelled peptide ion pairs was obtained using MASCOT Distiller combined with a proprietary software. Peptides displaying differences were selected for identification by MS. These preliminary results show the promise of our approach to identify potential biomarkers.
Leung
Kit-Yi
KY
Proteome Sciences plc, South Wing Laboratory, Institute of Psychiatry, London, UK. kit-yi.leung@proteomics.com
Lescuyer
Pierre
P
Campbell
James
J
Byers
Helen L
HL
Allard
Laure
L
Sanchez
Jean-Charles
JC
Ward
Malcolm A
MA
D000465:Algorithms; D000818:Animals; D015415:Biomarkers; D001798:Blood Proteins / Q000737:chemistry*; D019295:Computational Biology / Q000379:methods*; D030562:Databases, Protein; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D007477:Ions; D007554:Isotopes; D013058:Mass Spectrometry / Q000379:methods*; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D020543:Proteome; D040901:Proteomics / Q000379:methods; D012984:Software; D013997:Time Factors; D015854:Up-Regulation
D015415:Biomarkers; D001798:Blood Proteins; D007074:Immunoglobulin G; D007477:Ions; D007554:Isotopes; D010455:Peptides; D011506:Proteins; D020543:Proteome