title: Isolated del(14)(q21) in a case of precursor B-cell acute lymphoblastic leukemia.
abstract: We present a case of del(14)(q21) as a sole abnormality in a 4-year-old boy diagnosed with precursor B-cell acute lymphoblastic leukemia (pre-B ALL). To our knowledge, this is the first case of isolated del(14)(q21) in pre-B ALL. Two pretreatment bone marrow samples obtained 5 days apart were analyzed by cytogenetics. The G-banded karyotypes of the two samples were similar, differing only in the ratio of normal/abnormal metaphases detected. Both samples showed a del(14)(q21) as the only abnormality. Fluorescence in situ hybridization performed using the probes TEL/AML1 and immunoglobulin heavy chain (IGH) showed no fusion involving the TEL and AML1 genes and only a single IGH signal in 20% of the interphase cells analyzed.
authors.affiliation: Laboratory of Cytogenetics, Quest Diagnostics Nichols Institute, 14225 Newbrook Drive, Chantilly, VA 20151, USA. aurelia.m.meloni-ehrig@questdiagnostics.com
authors.forename: Aurelia M
authors.initials: AM
authors.lastname: Meloni-Ehrig
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Tirado
authors.forename: Kuoping
authors.initials: K
authors.lastname: Chen
authors.forename: Jennifer
authors.initials: J
authors.lastname: Jahn
authors.forename: Stephany
authors.initials: S
authors.lastname: Suchan
authors.forename: Jay
authors.initials: J
authors.lastname: Scheerle
authors.forename: Maria Gabriela
authors.initials: MG
authors.lastname: Crosby
authors.forename: Holly
authors.initials: H
authors.lastname: Meany
authors.forename: Nita
authors.initials: N
authors.lastname: Seibel
authors.forename: David
authors.initials: D
authors.lastname: Leitenberg
authors.forename: Deborah W
authors.initials: DW
authors.lastname: Heritage
authors.forename: Philip N
authors.initials: PN
authors.lastname: Mowrey
mesh_terms: D002675:Child, Preschool; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D050676:Core Binding Factor Alpha 2 Subunit; D017353:Gene Deletion; D015321:Gene Rearrangement; D006801:Humans; D007143:Immunoglobulin Heavy Chains; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007621:Karyotyping; D008297:Male; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D007143:Immunoglobulin Heavy Chains; D015514:Oncogene Proteins, Fusion; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein