Effects of R-(-)-BPAP on the expressions of neurotrophins and their receptors in mesencephalic slices.
R-(-)-1-(Benzofuran-2-yl)-2-propylaminopentane [R-(-)-BPAP] enhances electric field stimulation-induced release of catecholamine from isolated brain stem and ameliorates motor deficits in rats. We evaluated the effects of R-(-)-BPAP on the expression of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), and their receptors, trkB and p75(NTR) in rat mesencephalic slice cultures. Levels of mRNA and protein were measured at 48 h after R-(-)-BPAP treatment by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. R-(-)-BPAP significantly increased the mRNA and protein levels of BDNF, without affecting the level of NT-3 mRNA. In addition, R-(-)-BPAP significantly increased the mRNA level of trkB, but not that of p75(NTR). These effects of R-(-)-BPAP may result in enhanced BDNF/trkB signaling, and could thus underlie the potential neurotrophic and antidepressant actions of this drug.
Hirami
Chieko
C
Research Institute, Fujimoto Pharmaceutical Corporation, 1-3-40 Nishiotsuka, Matsubara, Osaka 580-0011, Japan.
Takahata
Kazue
K
Shimazu
Seiichiro
S
Yoneda
Fumio
F
Hayashi
Kyozo
K
Katsuki
Hiroshi
H
Akaike
Akinori
A
D000818:Animals; D001483:Base Sequence; D001572:Benzofurans / Q000494:pharmacology*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects*; D066298:In Vitro Techniques; D008636:Mesencephalon / Q000187:drug effects* / Q000378:metabolism; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020813:Receptor, trkB / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C404200:1-(benzofuran-2-yl)-2-propylaminopentane; D001572:Benzofurans; D019208:Brain-Derived Neurotrophic Factor; D017931:DNA Primers; D020932:Nerve Growth Factor; D020813:Receptor, trkB