Extracellular oxidases of the lignin-degrading fungus Panus tigrinus.
Two extracellular oxidases (laccases) were isolated from the extracellular fluid of the fungus Panus (Lentinus) tigrinus cultivated in low-nitrogen medium supplemented with birch sawdust. The enzymes were purified by successive chromatography on columns with TEAE-cellulose and DEAE-Toyopearl 650M. Both oxidases catalyze oxidation of pyrocatechol and ABTS. Moreover, oxidase 1 also catalyzes oxidation of guaiacol, o-phenylenediamine, and syringaldazine. The enzymes have identical pH (7.0) and temperature (60-65 degrees C) optimums. Absorption spectra of the oxidases differ from the spectra of typical "blue" laccases and are similar to the spectrum of yellow oxidase.
Cadimaliev
D A
DA
Department of Biotechnology, Biological Faculty, Ogarev Mordovian State University, Bolshevistskaya ul. 68, 430000 Saransk, Russia. biotech@moris.ru
Revin
V V
VV
Atykyan
N A
NA
Samuilov
V D
VD
D001487:Basidiomycota / Q000201:enzymology*; D002384:Catalysis; D002852:Chromatography, Ion Exchange; D042845:Laccase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D010084:Oxidation-Reduction; D013379:Substrate Specificity
D008031:Lignin; D042845:Laccase