title: Development of an infection-resistant bifunctionalized Dacron biomaterial.
abstract: A novel infection-resistant biomaterial was created by applying the antibiotic Ciprofloxacin (Cipro) to a recently developed bifunctionalized polyethylene terephthalate ("polyester," Dacron) material using textile-dyeing technology. Dacron was modified via exposure to ethylenediamine (EDA) to create amine and carboxylic acid sites within the polymer backbone. Cipro was applied to the bifunctionalized Dacron construct under varied experimental conditions, with resulting antimicrobial activity determined via zone of inhibition. Dacron segments treated at a liquor ratio of 20:1, with 5% Cipro on weight of fabric (owf), at pH 8 for 4 h at 70 degrees C followed by autoclaving showed antimicrobial activity for 78 days (length of study). Segments treated similarly but without autoclaving lost activity within 1 day. Dyeing time and temperature did not significantly affect antibiotic release/activity, but segments dyed at pHs higher or lower than 8 had less antimicrobial activity. The long-term infection resistance provided by this technique may answer major problems of infection from which implantable Dacron biomedical devices suffer.
authors.affiliation: Beth Israel Deaconess Medical Center, Vascular Surgery Research, 4 Blackfan Circle, Room 131, Boston, MA 02115, USA. aggarwal@bu.edu
authors.forename: Puja
authors.initials: P
authors.lastname: Aggarwal
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Phaneuf
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Bide
authors.forename: Kerry A
authors.initials: KA
authors.lastname: Sousa
authors.forename: Frank W
authors.initials: FW
authors.lastname: Logerfo
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry*; D002264:Carboxylic Acids / Q000737:chemistry; D002939:Ciprofloxacin / Q000737:chemistry / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008751:Methylene Blue / Q000737:chemistry / Q000494:pharmacology; D008956:Models, Chemical; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D011095:Polyethylenes / Q000737:chemistry; D011108:Polymers; D011140:Polyurethanes / Q000737:chemistry; D013053:Spectrophotometry; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D002264:Carboxylic Acids; D011093:Polyethylene Terephthalates; D011095:Polyethylenes; D011108:Polymers; D011140:Polyurethanes; D002939:Ciprofloxacin; D008751:Methylene Blue