title: [Transplantation of mouse bone marrow mesenchymal stem cells into the xenogeneic testis].
abstract: OBJECTIVE
  To study transplantation of mouse bone marrow mesenchymal stem cells (MSCs) into the xenogeneic testis.
  
  
  METHODS
  (1) The tibias and femurs were dissected from 5-6-week-old mice. The marrow in the tibias and femurs was flushed out with medium. MSCs were isolated, cultured and purified in vitro by Percoll density gradient centrifugation combined with adherent method. (2) MSCs of the third generation were adopted and marked with Hoechest33342 for observation, and then made into cell-suspending fluid. (3) The marked MSCs were transplanted into the testis of the xenogeneic mouse by testis net injection. The biopsies of the testis tissues were carried out at different time and made into frost slices at three sites for observation.
  
  
  RESULTS
  (1) A lot of purified MSCs were obtained at the third generation. (2) The nucleoli of the marked MSCs showed light-yellow under the fluoroscope. (3) Xenogeneic transplantation of mouse bone marrow MSCs by testis net injection was successful, without immunoreaction. On the 1 st day after transplantation, MSCs only concentratively distributed in the medial slices, the nucleoli being light-yellow; On the 1 st and 3 rd day, MSCs dispersively distributed in the medial slices; On the 6th, 9th and 12th day, MSCs presented in all the slices of the three sites, some ranging tubally; On the 15th and 18th day, the fluorescence of MSCs weakened; On the 21 st day, the fluorescence of MSCs disappeared.
  
  
  CONCLUSIONS
  Transplantation of mouse bone marrow MSCs into the xenogeneic testis by testis net injection is effective and feasible, without immunoreaction. MSCs can survive after transplantation.
authors.affiliation: Reproductive Medical Center, the Second Peoples Hospital of Guangzhou, Guangzhou, Guangdong 510150, China.
authors.forename: Feng-hua
authors.initials: FH
authors.lastname: Liu
authors.forename: Dong-zi
authors.initials: DZ
authors.lastname: Yang
authors.forename: Yi-feng
authors.initials: YF
authors.lastname: Wang
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Liang
authors.forename: Wen-ming
authors.initials: WM
authors.lastname: Peng
authors.forename: Chang-an
authors.initials: CA
authors.lastname: Cao
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Ma
authors.forename: Xi-gu
authors.initials: XG
authors.lastname: Chen
authors.forename: Zhong-min
authors.initials: ZM
authors.lastname: Guo
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002478:Cells, Cultured; D006085:Graft Survival; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013737:Testis / Q000601:surgery*