Stress proteins induced by exposure to sublethal levels of heavy metals in sea bream (Sparus sarba) blood cells.
Blood cells freshly collected from silver sea bream (Sparus sarba) were exposed in vitro to different sublethal concentrations of cadmium(II), lead(II) or chromium(VI). HSP70 stress proteins were significantly overexpressed after exposure to metal concentration as low as 0.1 microM. Under our experimental conditions, no overexpression of metallothioneins in blood cells was evidenced. Our results show that fish blood cells may constitute an interesting biological model for experimental and applied toxicology, especially in the case of environmental pollution.
Fulladosa
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E
Department of chemical Engineering, Universitat de Girona, Avda. Lluís Santaló, Campus de Montilivi, 17071 Girona, Spain. elena.fulladosa@udg.es
Deane
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E
Ng
A H Y
AH
Woo
N Y S
NY
Murat
J C
JC
Villaescusa
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I
D000818:Animals; D001773:Blood Cells; D002104:Cadmium / Q000633:toxicity*; D002857:Chromium / Q000633:toxicity*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D007854:Lead / Q000633:toxicity*; D021541:Sea Bream / Q000097:blood*; D018675:Toxicity Tests / Q000379:methods; D014874:Water Pollutants, Chemical / Q000633:toxicity
D018840:HSP70 Heat-Shock Proteins; D014874:Water Pollutants, Chemical; D002104:Cadmium; D002857:Chromium; C074702:chromium hexavalent ion; D007854:Lead