Induction of xylanases by sugar cane bagasse at different cell densities of Cellulomonas flavigena.
The effect of cell density on xylanolytic activity and productivity of Cellulomonas flavigena was evaluated under two different culturing conditions: fed-batch culture with discontinuous feed of sugar cane bagasse (SCB; condition 1) and glycerol fed-batch culture followed by addition of SBC as xylanases inducer (condition 2). The enzymatic profile of xylanases was similar in both systems, regardless of the initial cell density at time of induction. However, the xylanolytic activity changed with initial cell density at the time of induction (condition 2). The maximum volumetric xylanase activity increased with increased initial cell density from 4 to 34 g l(-1) but decreased above this value. The largest total volumetric xylanase productivity under condition 2 (1.3 IU ml(-1) h(-1)) was significantly greater compared to condition 1 (maximum 0.6 IU ml(-1) h(-1)). Consequently, induction of xylanase activity by SCB after growing of C. flavigena on glycerol at intermediate cell density can be a feasible alternative to improve activity and productivity of xylanolytic enzymes.
Amaya-Delgado
L
L
Department of Biotechnology and Bioengineering, CINVESTAV, San Pedro Zacatenco, México DF, Mexico.
Vega-Estrada
J
J
Flores-Cotera
L B
LB
Dendooven
L
L
Hidalgo-Lara
M E
ME
Montes-Horcasitas
M C
MC
D040143:Cellulomonas / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D002482:Cellulose / Q000494:pharmacology*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D004790:Enzyme Induction; D005990:Glycerol / Q000378:metabolism
D002482:Cellulose; C027433:bagasse; D043364:Endo-1,4-beta Xylanases; D005990:Glycerol