Protective effect of propofol against kainic acid-induced lipid peroxidation in mouse brain homogenates: comparison with trolox and melatonin.
This study compared the effectiveness of propofol with that of trolox and melatonin for reduction of lipid peroxidation in vitro. Lipid peroxidation was induced by addition of kainic acid (KA; 10 mM), hydrogen peroxide (H2O2; 10 mM), or ferrous ammonium sulfate (5 microM) to mouse brain homogenate, and thiobarbituric acid-reactive substances (TBA-RS) were used as a marker of lipid peroxidation. Propofol, trolox, and melatonin reduced KA-, H2O2-, and ferrous ammonium sulfate-induced lipid peroxidation in a concentration-dependent manner. In reducing KA-induced lipid peroxidation, 50% inhibitory concentration (IC50) values of antioxidants were as follows: propofol (11.33 mM), trolox (4.00 mM), and melatonin (9.72 mM). In reducing H2O2-induced lipid peroxidation, IC50 values of antioxidants were as follows: propofol (56.86 mM), trolox (33.34 mM), and melatonin (26.63 mM). In reducing ferrous ion-induced lipid peroxidation, IC50 values of antioxidants were as follows: propofol (49.57 mM), trolox (60.35 mM), and melatonin (22.02 mM). Under the in vitro conditions of this experiment, propofol was an excellent and a very potent antioxidant in inhibiting KA-, H2O2-, and ferrous ion-induced lipid peroxidation in mouse brain homogenates. We conclude that the antioxidant properties of propofol at clinically relevant anesthetic concentrations may have a neuroprotective effect.
Lee
Hyung
H
Department of Neurology, School of Medicine, Keimyung University, Taegu, South Korea.
Jang
Young-Ho
YH
Lee
Seong-Ryong
SR
D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001923:Brain Chemistry / Q000187:drug effects*; D002839:Chromans / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000633:toxicity*; D005296:Ferrous Compounds / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008550:Melatonin / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D015742:Propofol / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D018686:Anesthetics, Intravenous; D000975:Antioxidants; D002839:Chromans; D018691:Excitatory Amino Acid Antagonists; D005296:Ferrous Compounds; D000644:Quaternary Ammonium Compounds; D017392:Thiobarbituric Acid Reactive Substances; C038178:ammonium ferrous sulfate; D006861:Hydrogen Peroxide; D008550:Melatonin; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; D007608:Kainic Acid; D015742:Propofol