title: Speciation analysis of inorganic arsenic by microchip capillary electrophoresis coupled with hydride generation atomic fluorescence spectrometry.
abstract: A novel method for speciation analysis of inorganic arsenic was developed by on-line hyphenating microchip capillary electrophoresis (chip-CE) with hydride generation atomic fluorescence spectrometry (HG-AFS). Baseline separation of As(III) and As(V) was achieved within 54 s by the chip-CE in a 90 mm long channel at 2500 V using a mixture of 25 mmol l(-1) H3BO3 and 0.4 mmol l(-1) CTAB (pH 8.9) as electrolyte buffer. The precisions (RSD, n=5) ranged from 1.9 to 1.4% for migration time, 2.1 to 2.7% for peak area, and 1.8 to 2.3% for peak height for the two arsenic species at 3.0 mg l(-1) (as As) level. The detection limits (3sigma) for As(III) and As(V) based on peak height measurement were 76 and 112 microg l(-1) (as As), respectively. The recoveries of the spikes (1 mg l(-1) (as As) of As(III) and As(V)) in four locally collected water samples ranged from 93.7 to 106%.
authors.affiliation: Research Centerfor Analytical Sciences, College of Chemistry, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Wang
authors.forename: Xiu-Ping
authors.initials: XP
authors.lastname: Yan
authors.forename: Rong-Guo
authors.initials: RG
authors.lastname: Su
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
mesh_terms: D001128:Argon; D001152:Arsenicals / Q000032:analysis*; D001894:Borohydrides; D046209:Electrophoresis, Microchip / Q000379:methods*; D009862:Online Systems / Q000295:instrumentation; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D001152:Arsenicals; D001894:Borohydrides; C028371:potassium borohydride; D001128:Argon