[Effect of hepatocyte growth factor on oxygen-glucose deprived injury of astrocytes].
OBJECTIVE
To explore the effect of hepatocyte growth factor (HGF) on oxygen-glucose deprived injury and apoptosis of astrocytes.


METHODS
The injury of primary cultured rat cerebral cortical astrocytes was induced by oxygen-glucose deprivation. Astrocytes were treated with HGF at various final concentrations of 20 - 100 ng/mL. The cell damage and viability were evaluated by the lactate dehydrogenase (LDH) released rate and the 3- (4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) conversion method. Detection of apoptotic cells was determined by the flow cytometry, and the ultrastructure was observed by the transmission electron microscope.


RESULTS
Oxygen-glucose deprivation increased the LDH release rate, decreased the cell viability and increased the number of apoptotic astrocytes. While exposed to HGF at the same condition, the LDH release rate decreased, the cell viability increased, and the percentage of apoptotic cells decreased (P <0.05). The maximum protective effect of HGF was observed at 60 ng/mL.


CONCLUSIONS
HGF can protect cultured astrocytes from oxygen-glucose deprived injury, and attenuate the apoptosis of astrocytes in a dose-dependent manner.
He
Fang
F
Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Wu
Li-Xiang
LX
Liu
Fa-Yi
FY
Cao
Li
L
Yang
Li-Juan
LJ
Zhou
Xuan
X
Zhao
Yan
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001253:Astrocytes / Q000473:pathology*; D015687:Cell Hypoxia; D005947:Glucose / Q000494:pharmacology; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*
D017228:Hepatocyte Growth Factor; D005947:Glucose