[Altered expression of the HSD17B4 gene in esophageal squamous cell carcinoma and loss of heterozygosity analysis].
OBJECTIVE
To investigate the alteration of the gene HSD17B4 in esophageal squamous cell carcinoma and its potential significance.


METHODS
The mRNA expression and loss of heterozygosity (LOH) of HSD17B4 in 40 primary esophageal tumors were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and microsatellite analysis with the intragenic marker D5S1384 of the gene.


RESULTS
The frequencies of allelic loss of D5S1384 and the rate of down-regulation of gene HSD17B4 were 46.2% and 62.5%, respectively.


CONCLUSIONS
HSD17B4 may be a candidate tumor suppressor gene associated with esophageal squamous cell carcinoma.
Li
Xiao-dong
XD
State Key Laboratory of Molecular Oncology, Cancer Institute, CAMS and PUMC, Beijing 100021, China.
Liu
Xiang-yang
XY
Huang
Xiao-ping
XP
Fu
Jian-hua
JH
Hu
Yi
Y
Xu
Xin
X
Cai
Yan
Y
Han
Ya-ling
YL
Rong
Tie-hua
TH
Wu
Min
M
Zhan
Qi-min
QM
Wang
Ming-rong
MR
D015067:17-Hydroxysteroid Dehydrogenases / Q000096:biosynthesis / Q000235:genetics*; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D015536:Down-Regulation; D004746:Enoyl-CoA Hydratase / Q000096:biosynthesis / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006801:Humans; D006836:Hydro-Lyases; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000235:genetics*; D064029:Peroxisomal Multifunctional Protein-2; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D009097:Multienzyme Complexes; D012333:RNA, Messenger; D015067:17-Hydroxysteroid Dehydrogenases; D006836:Hydro-Lyases; D064029:Peroxisomal Multifunctional Protein-2; C104587:HSD17B4 protein, human; D004746:Enoyl-CoA Hydratase