title: Kidneys extract BNP and NT-proBNP in healthy young men.
abstract: Renal metabolism of the cardiac marker NH2-terminal-pro-brain natriuretic peptide (NT-proBNP) has been suggested. Therefore, we determined the renal extraction ratios of NT-proBNP and its bioactive coproduct brain natriuretic peptide (BNP) at rest and during exercise. In addition, the cerebral ratios were evaluated. Ten young healthy men were investigated at baseline, during moderate cycle exercise (heart rate: 140, Borg scale: 14-15), and in the recovery with BNP and NT-proBNP measured from the brachial artery and the jugular and renal veins, and the renal and cerebral extraction ratios (Ext-Ren and Ext-Cer, respectively) were calculated. Cardiac output, stroke volume, heart rate, mean arterial pressures, and estimated glomerular filtration were determined. BNP and NT-proBNP were extracted by the kidneys but not by the brain. We observed no effect of exercise. The mean values (+/- SE) of Ext-Ren of NT-proBNP were similar (0.19 +/- 0.05, 0.21 +/- 0.06, and 0.12 +/- 0.03, respectively) during the three sessions (P > 0.05). Also the Ext-Ren of BNP were similar (0.18 +/- 0.07, 0.15 +/- 0.11, and 0.14 +/- 0.06, respectively; P > 0.05). There were no significant differences between Ext-Ren of BNP and NT-proBNP during the three sessions (P > 0.05). The Ext-Cer of both peptides varied insignificantly between -0.21 +/- 0.15 and 0.11 +/- 0.08. The renal extraction ratio of both BNP and NT-proBNP is approximately 0.15-0.20. There is no cerebral extraction, and short-term moderate exercise does not affect these values. Our findings suggest that the kidneys extract BNP and NT-proBNP to a similar extent in healthy young men.
authors.affiliation: Dept. of Endocrinology and Cardiology, Frederiksberg Univ. Hospital, Ndr Fasanvej 57-59, DK-2000 Frederiksberg, Denmark. m.schou@dadlnet.dk
authors.forename: Morten
authors.initials: M
authors.lastname: Schou
authors.forename: Mads K
authors.initials: MK
authors.lastname: Dalsgaard
authors.forename: Otto
authors.initials: O
authors.lastname: Clemmesen
authors.forename: Ellen A
authors.initials: EA
authors.lastname: Dawson
authors.forename: Chie C
authors.initials: CC
authors.lastname: Yoshiga
authors.forename: Henning B
authors.initials: HB
authors.lastname: Nielsen
authors.forename: Finn
authors.initials: F
authors.lastname: Gustafsson
authors.forename: Per R
authors.initials: PR
authors.lastname: Hildebrandt
authors.forename: Niels H
authors.initials: NH
authors.lastname: Secher
mesh_terms: D000328:Adult; D001794:Blood Pressure; D001921:Brain / Q000378:metabolism; D015444:Exercise / Q000502:physiology*; D005919:Glomerular Filtration Rate; D006339:Heart Rate; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008297:Male; D020097:Natriuretic Peptide, Brain / Q000097:blood / Q000378:metabolism*; D010446:Peptide Fragments / Q000097:blood / Q000378:metabolism*; D011498:Protein Precursors / Q000097:blood / Q000378:metabolism; D013318:Stroke Volume
chemical_list: D010446:Peptide Fragments; D011498:Protein Precursors; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain