title: Fluorescence anisotropy studies of molecularly imprinted polymers.
abstract: A molecularly imprinted polymer (MIP) is a biomimetic material that can be used as a biochemical sensing element. We studied the steady-state and time-resolved fluorescence and fluorescence anisotropy of anthracene-imprinted polyurethane. We compared MIPs with imprinted analytes present, MIPs with the imprinted analytes extracted, MIPs with rebound analytes, non-imprinted control polymers (non-MIPs) and non-MIPs bound with analytes to understand MIP's binding behaviour. MIPs and non-MIPs had similar steady-state fluorescence anisotropy in the range 0.11-0.24. Anthracene rebound in MIPs and non-MIPs had a fluorescence lifetime of tau = 0.64 ns and a rotational correlation time of phi(F) = 1.2-1.5 ns, both of which were shorter than that of MIPs with imprinted analytes present (tau = 2.03 ns and phi(F) = 2.7 ns). The steady-state anisotropy of polymer solutions increased exponentially with polymerization time and might be used to characterize the polymerization extent in situ.
authors.affiliation: Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR, USA.
authors.forename: Yin-Chu
authors.initials: YC
authors.lastname: Chen
authors.forename: Zheming
authors.initials: Z
authors.lastname: Wang
authors.forename: Mingdi
authors.initials: M
authors.lastname: Yan
authors.forename: Scott A
authors.initials: SA
authors.lastname: Prahl
mesh_terms: D005454:Fluorescence Polarization; D011108:Polymers / Q000737:chemistry*
chemical_list: D011108:Polymers