Anicemycin, a new inhibitor of anchorage-independent growth of tumor cells from Streptomyces sp. TP-A0648.
The anchorage-independence of cells is closely related to their tumorigenicity. In the screening of inhibitors of anchorage-independent growth of tumor cells, anicemycin was isolated from the fermentation broth of an actinomycete strain TP-A0648. The producing strain was isolated from a leaf of Aucuba japonica collected in Toyama, Japan and identified as Streptomyces sp. based on the taxonomic data. The structure of anicemycin was elucidated as a new analog of spicamycin by NMR and MS analysis. Anicemycin inhibited the anchorage-independent growth of the human ovary cancer SKOV-3 cells with an IC50 of 0.015 microM about three times more potently than their anchorage-dependent growth.
Igarashi
Yasuhiro
Y
Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Imizu, Toyama 939-0398, Japan. yas@pu-toyama.ac.jp
Ootsu
Koushirou
K
Onaka
Hiroyasu
H
Fujita
Tsuyoshi
T
Uehara
Yoshimasa
Y
Furumai
Tamotsu
T
D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004354:Drug Screening Assays, Antitumor; D005285:Fermentation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D008958:Models, Molecular; D018515:Plant Leaves / Q000382:microbiology; D011684:Purine Nucleosides / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D011684:Purine Nucleosides; C502978:anicemycin