title: Effect of root canal filling/sealer systems on apical endotoxin penetration: a coronal leakage evaluation.
abstract: Endotoxin, elaborated by gram-negative organisms, is an important factor in apical periodontitis. The objective of this study was to evaluate the magnitude of endotoxin penetration through root canal treated teeth using a dual chamber model system. Forty-four maxillary anterior teeth were prepared endodontically and canals filled either by lateral condensation or a warm thermoplasticized technique in combination with either Roth's 801 or AH 26 sealer. Teeth were suspended in the model system with a mixed anaerobic bacterial suspension in the upper chamber and HBSS in the lower chamber. The QCL-1000 LAL assay was used to measure endotoxin at 0, 1, 7, 14, and 21 days. Response feature analysis using trapezoidal area under the curve was performed; the four treatment groups were compared using nonparametric methods. Groups differed (p = 0.028), with thermoplasticized root canal filling/Roth's 801 sealer permitting the least apical endotoxin penetration. Results suggest that Roth's 801 sealer may have a role in inhibiting endotoxin penetration.
authors.affiliation: Department of Endodontics, College of Dentistry, University of Iowa, Iowa City, IA 52242, USA. anne-williamson@uiowa.edu
authors.forename: Anne E
authors.initials: AE
authors.lastname: Williamson
authors.forename: Deborah V
authors.initials: DV
authors.lastname: Dawson
authors.forename: David R
authors.initials: DR
authors.lastname: Drake
authors.forename: Richard E
authors.initials: RE
authors.lastname: Walton
authors.forename: Eric M
authors.initials: EM
authors.lastname: Rivera
mesh_terms: D000704:Analysis of Variance; D001421:Bacteria, Anaerobic; D001729:Bismuth; D003763:Dental Leakage; D004338:Drug Combinations; D004731:Endotoxins; D004853:Epoxy Resins; D006171:Gutta-Percha; D012387:Root Canal Filling Materials; D012389:Root Canal Obturation / Q000379:methods*; D012834:Silver; D018709:Statistics, Nonparametric; D014025:Titanium; D019227:Tooth Apex; D015035:Zinc Oxide-Eugenol Cement
chemical_list: D004338:Drug Combinations; D004731:Endotoxins; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; D012834:Silver; C028044:epoxy resin AH-26; C074935:Roth's 801 Sealer; D006171:Gutta-Percha; D014025:Titanium; D001729:Bismuth