title: Activity, diversity and population size of ammonia-oxidising bacteria in oil-contaminated landfarming soil.
abstract: Chemolithotrophic ammonia-oxidising bacteria (AOB) present in oil-contaminated landfarming soil were studied over two growing seasons in 1999 and 2000. The number of AOB (4-9 x 10(5) cellsg(-1) of dry soil) determined with the quantitative polymerase chain reaction (real-time PCR) and the rate of potential ammonium oxidation (0.05-0.28 microg NO2(-)-N g(-1) of dry soil h(-1)) indicated the presence of stable AOB populations. Denaturing gradient gel electrophoresis (DGGE) profiling and sequence analysis of PCR-amplified AOB 16S rRNA genes showed dominance of Nitrosospira-like sequences in clusters 2 and 3. The present results from the chronically oil-contaminated landfarming soil support the suggested importance of Nitrosospira-like AOB in terrestrial environments.
authors.affiliation: Department of Ecological and Environmental Sciences, University of Helsinki, Niemenkatu 73, Lahti, Finland. jukka.kurola@helsinki.fi
authors.forename: Jukka
authors.initials: J
authors.lastname: Kurola
authors.forename: Mirja
authors.initials: M
authors.lastname: Salkinoja-Salonen
authors.forename: Tuula
authors.initials: T
authors.lastname: Aarnio
authors.forename: Jenni
authors.initials: J
authors.lastname: Hultman
authors.forename: Martin
authors.initials: M
authors.lastname: Romantschuk
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D014644:Genetic Variation; D043369:Nitrosomonadaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D009821:Oils / Q000378:metabolism*; D010084:Oxidation-Reduction; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D009821:Oils; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D000641:Ammonia