title: New genotype-phenotype linkages for directed evolution of functional proteins.
abstract: The key practical consideration in directed evolution of functional biomolecules is the linkage of genotype and phenotype. In vitro selections offer the potential to select from libraries with up to 10(10)-10(14) members, with fewer constraints than current cell-based selections. New approaches such as mRNA display, ribosome display and in vitro compartmentalisation have complementary areas of application in selections for binding or catalysis.
authors.affiliation: Department of Biochemistry, Cambridge University, Cambridge CB2 1GA, UK.
authors.forename: Hans
authors.initials: H
authors.lastname: Leemhuis
authors.forename: Viktor
authors.initials: V
authors.lastname: Stein
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Griffiths
authors.forename: Florian
authors.initials: F
authors.lastname: Hollfelder
mesh_terms: D019020:Directed Molecular Evolution; D005838:Genotype; D010641:Phenotype; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D011506:Proteins