[A study on the hairy root culture and antitumor alkaloids production of Catharanthus roseus].
OBJECTIVE
To establish transformation system and obtain alkaloids from the hairy root of Catharanthus roseus.


METHODS
Hairy roots were obtained by infecting the different explants of C. roseus. Culture conditions of hairy root were optimized.


RESULTS
The best transformation condition was leaf infected by two-day's pre-culture and two-day's co-culture and additional A(S) (hydroxyacetosyringone) 100 mg x L(-1). The inducing rate of hairy root was up to 86.25%. The best condition of hairy root culture was MS medium with sucrose as carbon material and lactalbumin as nitron material. The analysis result showed that the contents of total alkaloids in hairy roots were higher than explants and calli.


CONCLUSIONS
Hairy root of C. roseus will be useful for the production of active components in C. roseus.
Sun
Min
M
School of Life Science, Southwest Normal University, Chongqing 400715, China.
Zeng
Jian-jun
JJ
D000470:Alkaloids / Q000032:analysis*; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis*; D030008:Catharanthus / Q000737:chemistry / Q000254:growth & development* / Q000382:microbiology; D003470:Culture Media; D007768:Lactalbumin; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development / Q000382:microbiology; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development* / Q000382:microbiology; D012231:Rhizobium; D013395:Sucrose; D046509:Tissue Culture Techniques / Q000379:methods; D014747:Vinblastine / Q000032:analysis; D014750:Vincristine / Q000032:analysis
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D003470:Culture Media; D013395:Sucrose; D014750:Vincristine; D014747:Vinblastine; D007768:Lactalbumin