Antiangiogenic activity of nasunin, an antioxidant anthocyanin, in eggplant peels.
Nasunin, delphinidin-3-(p-coumaroylrutinoside)-5-glucoside, an antioxidant anthocyanin isolated from eggplant peels, was demonstrated as an angiogenesis inhibitor. Nasunin at higher 10 microM suppressed microvessel outgrowth in an ex vivo angiogenesis assay using a rat aortic ring. The effect of nasunin was examined in various in vitro angiogenesis models using human umbilical vein endothelial cells (HUVECs). Nasunin suppressed HUVEC proliferation in a dose-dependent manner (50-200 microM); however, it had no significant effect on HUVEC chemotaxis in a Boyden chamber assay and HUVEC tube formation on a reconstituted basement membrane. These results imply that nasunin with both antioxidant and antiangiogenic activities might be useful to prevent angiogenesis-related diseases.
Matsubara
Kiminori
K
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan. kmatsuba@fhw.oka-pu.ac.jp
Kaneyuki
Takao
T
Miyake
Tsuyoshi
T
Mori
Masaharu
M
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D000872:Anthocyanins / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants; D001011:Aorta / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D032323:Solanum melongena / Q000737:chemistry*; D014471:Umbilical Veins / Q000187:drug effects
D020533:Angiogenesis Inhibitors; D000872:Anthocyanins; D000975:Antioxidants; C118727:nasunin