title: Pharmacokinetics of guanfu base I, an active metabolite of guanfu base A, in rats.
abstract: A liquid chromatographic-mass spectrometric (LC-MS) method was developed and validated for determination of guanfu base I (GFI), and the pharmacokinetics of GFI in Sprague-Dawley rats was examined. The method was linear in the 0.05-20 mug/ml concentration range (r=0.9994). The recovery of guanfu base I was more than 80%. The intraday and interday precision, expressed as the relative standard deviation (RSD), was generally good (<15%). After i.v. dosing, plasma GFI concentration declined in a bi-phasic manner with a terminal elimination half-life of 2.49 h. The total plasma clearance values was 1.46 l/h/kg. After oral dosing, the plasma GFI concentration reached a maximum within 0.5 h. The absolute bioavailability of GFI was 71.31%.
authors.affiliation: Key Laboratory of Drug Metabolism & Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Li
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
authors.forename: Sujun
authors.initials: S
authors.lastname: Wang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Sun
authors.forename: Jinghan
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D006207:Half-Life; D006576:Heterocyclic Compounds, 4 or More Rings / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D006576:Heterocyclic Compounds, 4 or More Rings; C040615:Guan-fu base A