title: The Drosophila gene CG9918 codes for a pyrokinin-1 receptor.
abstract: The database from the Drosophila Genome Project contains a gene, CG9918, annotated to code for a G protein-coupled receptor. We cloned the cDNA of this gene and functionally expressed it in Chinese hamster ovary cells. We tested a library of about 25 Drosophila and other insect neuropeptides, and seven insect biogenic amines on the expressed receptor and found that it was activated by low concentrations of the Drosophila neuropeptide, pyrokinin-1 (TGPSASSGLWFGPRLamide; EC50, 5 x 10(-8) M). The receptor was also activated by other Drosophila neuropeptides, terminating with the sequence PRLamide (Hug-gamma, ecdysis-triggering-hormone-1, pyrokinin-2), but in these cases about six to eight times higher concentrations were needed. The receptor was not activated by Drosophila neuropeptides, containing a C-terminal PRIamide sequence (such as ecdysis-triggering-hormone-2), or PRVamide (such as capa-1 and -2), or other neuropeptides and biogenic amines not related to the pyrokinins. This paper is the first conclusive report that CG9918 is a Drosophila pyrokinin-1 receptor gene.
authors.affiliation: Department of Cell Biology and Comparative Zoology, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cazzamali
authors.forename: Malene
authors.initials: M
authors.lastname: Torp
authors.forename: Frank
authors.initials: F
authors.lastname: Hauser
authors.forename: Michael
authors.initials: M
authors.lastname: Williamson
authors.forename: Cornelis J P
authors.initials: CJ
authors.lastname: Grimmelikhuijzen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D004330:Drosophila / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005091:Exons / Q000235:genetics; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism*; D010802:Phylogeny; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D029721:Drosophila Proteins; D009479:Neuropeptides; D043562:Receptors, G-Protein-Coupled; C079229:pyrokinin; C504361:pyrokinin-1 receptor, Drosophila