[Comparison of PCR and isoenzyme analysis in identification of Anopheles minimus A and C].
OBJECTIVE
To compare the PCR and isoenzyme methods in identification of Anopheles minimus A and C.


METHODS
Samples of An. minimus collected in the field were identified from An. aconitus and An. jeyporiensis by PCR-RFLP after morphological examination, then classified by PCR-ASA. The mosquitoes identified by this method were further analyzed for isoenzyme to observe the difference, which was previously used as a technique to classify An. minimus A and C.


RESULTS
PCR method can differentiate An. minimus A and C simply and quickly. For Isoenzyme method, only EST allozyme pattern displayed difference.


CONCLUSIONS
PCR method is better than isoenzyme analysis in differentiating An. minimus A and C.
Zheng
Bin
B
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis, Shanghai 200025, China.
Tang
Lin-Hua
LH
Ma
Ya-Jun
YJ
Wang
Xue-Zhong
XZ
Zhou
Shui-Sen
SS
Shi
Wen-Qi
WQ
D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D007527:Isoenzymes / Q000032:analysis; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D007527:Isoenzymes