title: Resistance to intestinal Entamoeba histolytica infection is conferred by innate immunity and Gr-1+ cells.
abstract: Establishment of intestinal infection with Entamoeba histolytica depends on the mouse strain; C57BL/6 mice are highly resistant, and C3H/HeJ mice are relatively susceptible. We found that resistance to intestinal infection was independent of lymphocyte activity or H-2 haplotype and occurred in the first hours to days postchallenge according to in vivo imaging. At 18 h postchallenge, the ceca of resistant C57BL/6 mice were histologically unremarkable, in contrast to the severe inflammation observed in susceptible C3H/HeJ mice. Comparison of cecal gene expression in C3H/HeJ and C57BL/6 mice demonstrated that there was parasite-induced upregulation of proinflammatory and neutrophil chemotaxis transcripts and there was downregulation of transforming growth factor beta signaling molecules. Pretreatment with dexamethasone abrogated the partial resistance of C3H/HeJ or CBA mice through an innate, lymphocyte-independent mechanism, but it had no effect on the high-level resistance of C57BL/6 mice. Similarly, administration of a neutrophil-depleting anti-Gr-1 monoclonal antibody (RB6-8C5) decreased the partial resistance of CBA mice and led to severe pathology compared to control antibody-treated mice, but it had no effect on C57BL/6 resistance. These data indicate that there are discrete mechanisms of innate resistance to E. histolytica depending on the host background and, in contrast to other reports, imply that neutrophils are protective and not damaging in intestinal amebiasis.
authors.affiliation: Division of Infectious Diseases and International Health, MR4 Building, Room 2144, University of Virginia, Charlottesville, VA 22908-1363, USA.
authors.forename: Amon
authors.initials: A
authors.lastname: Asgharpour
authors.forename: Carol
authors.initials: C
authors.lastname: Gilchrist
authors.forename: Duza
authors.initials: D
authors.lastname: Baba
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Hamano
authors.forename: Eric
authors.initials: E
authors.lastname: Houpt
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004404:Dysentery, Amebic / Q000276:immunology*; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000276:immunology*; D020869:Gene Expression Profiling; D006183:H-2 Antigens / Q000276:immunology; D007113:Immunity, Innate / Q000187:drug effects; D007249:Inflammation / Q000276:immunology / Q000469:parasitology; D007422:Intestines / Q000276:immunology / Q000469:parasitology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D016513:Mice, SCID
chemical_list: D000893:Anti-Inflammatory Agents; D006183:H-2 Antigens; D003907:Dexamethasone