[Dynamic release of tinidazole from the degradable PLLA-tinidazole blend gel].
OBJECTIVE
To determine the dynamic release of tinidazole from the degradable poly-lactic acid (PLLA)-tinidazole blend gel (a periodontal local drug delivery) by high-performance liquid chromatography (HPLC) in vitro and to observe the polylactic acid (as a vector of tinidazole) how to affect the release of tinidazole.


METHODS
HPLC analysis was conducted. The operating conditions were C18-ODS-A column, water-methanol-acetic acid as mobile phase at a flow rate of 1.5 ml/min and a detection wavelength at 310 nm and a column temperature at 30 degrees C.


RESULTS
The retention time of tinidazole was about 12 min. There was good linear relationship between the concentrations of tinidazole in blend gel and their peak areas in the ranges of 5 - 500 mg/L. The regression reaction was y = 0.836 + 8.452 2 x 10(-5) x (r = 0.998 6). RSD was 0.2%. Polylactic acid had no influence on the determination of tinidazole. The daily average release of tinidazole was 5.60%. The cumulative release of tinidazole was about 78.40% in 14 days.


CONCLUSIONS
The in vitro drug release analysis is an useful method in evaluating the performance of local drug delivery system. HPLC analysis is a simple and accurate method with good reproducibility.
Liu
Juan
J
Laboratory of Oral Biomedical Engineering, Ministry of Education, Sichuan University, Chengdu 610041, China
Xiao
Li-Ying
LY
Li
Wei
W
Ding
Yi
Y
Zhang
Ping
P
Yuan
Ming-Long
ML
D002851:Chromatography, High Pressure Liquid; D019344:Lactic Acid; D011091:Polyesters; D011108:Polymers; D015203:Reproducibility of Results; D014011:Tinidazole
D011091:Polyesters; D011108:Polymers; D014011:Tinidazole; D019344:Lactic Acid; C033616:poly(lactide)