[Bone marrow infiltration in B-cell non-Hodgkin's lymphomas: comparison between flow cytometry and bone marrow biopsy].
A comparison of flow cytometry (FC) and bone marrow biopsy (BMB) to evaluate bone marrow infiltration was made in 114 patients suffering from B-cell non-Hodgkin's lymphomas (NHLs; 51 at diagnosis, 63 during post-therapy follow-up). The following parameters were indicative of bone marrow infiltration: altered surface k/l ratio; specific immunophenotypic pattern in particular NHLs (CLL, mantle cell lymphoma, hairy cell leukemia). FC and BMB agreed in 89.5% of cases (i.e. both showed 48 positive and 54 negative cases). In discordant cases (7.9%) and in cases not evaluable by FC (2.6%) IgH rearrangement and bcl-1 gene expression, both evaluated by PCR methods, were used to detect bone marrow infiltration with higher precision. These results show that a more complex analysis of bone marrow is needed to diagnose bone marrow infiltration, particularly in samples with minimal residual disease.
Carulli
Giovanni
G
Divisione di Ematologia, Dipartimento di Oncologia, dei Trapianti e delle Nuove Tecnologie in Medicina, Università, Ospedale S. Chiara, Pisa. giovanni_carulli@virgilio.it
Canigiani
Silvia
S
Volpini
Maila
M
Ciancia
Eugenio M
EM
Galimberti
Sara
S
Zucca
Alessandra
A
Marini
Alessandra
A
Buda
Gabriele
G
Petrini
Mario
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle / Q000379:methods; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology*; D001856:Bone Marrow Examination; D019046:Bone Marrow Neoplasms / Q000276:immunology / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D019940:Genes, bcl-1; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D016393:Lymphoma, B-Cell / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012189:Retrospective Studies