title: Identification and characterization of two antisense transcripts from the major immediate early region of rat cytomegalovirus.
abstract: We have identified and characterized two antisense transcripts from the rat cytomegalovirus (RCMV) major immediate early (MIE) region. These transcripts, designated IE-AS1 and IE-AS2, are complementary to part of the sense IE1 transcript. The IE-AS transcripts were first detected in peripheral blood leukocytes (PBL) of RCMV-infected rats at 7 days post-infection (pi) in the absence of IE1 transcription. Nevertheless, both the IE1 and IE-AS transcripts were found at the same time in the salivary glands of RCMV-infected rats at 7 and 120 days pi as well as in RCMV-infected rat embryo fibroblasts (REFs) at 48 h pi.
authors.affiliation: Department of Medical Microbiology, Cardiovascular Research Institute Maastricht (CARIM), University of Maastricht, Maastricht, The Netherlands.
authors.forename: K W R
authors.initials: KW
authors.lastname: van Cleef
authors.forename: M J
authors.initials: MJ
authors.lastname: Blok
authors.forename: K G M
authors.initials: KG
authors.lastname: Savelkouls
authors.forename: G E L M
authors.initials: GE
authors.lastname: Grauls
authors.forename: C A
authors.initials: CA
authors.lastname: Bruggeman
authors.forename: C
authors.initials: C
authors.lastname: Vink
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D005347:Fibroblasts / Q000821:virology; D017874:Immediate-Early Proteins / Q000235:genetics*; D007962:Leukocytes / Q000821:virology; D008969:Molecular Sequence Data; D018146:Muromegalovirus / Q000235:genetics*; D016372:RNA, Antisense / Q000032:analysis* / Q000235:genetics; D018075:RNA, Complementary / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D051381:Rats; D012469:Salivary Glands / Q000821:virology
chemical_list: D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D016372:RNA, Antisense; D018075:RNA, Complementary; D012333:RNA, Messenger; D012367:RNA, Viral; C044582:immediate-early proteins, cytomegalovirus