title: On-line identification of sugarcane (Saccharum officinarum L.) methoxyflavones by liquid chromatography-UV detection using post-column derivatization and liquid chromatography-mass spectrometry.
abstract: Sugarcane (Saccharum officinarum L., Gramineae) bagasse and leaves were investigated for their flavonoid content and transgenic sugarcane ("Bowman-Birk" and "Kunitz") was compared with non-modified ("control") plants. Analyses were carried out by high-performance liquid chromatography coupled to diode array UV detection (LC/UV), also using post-column addition of shift reagents, and tandem MS (atmospheric pressure chemical ionization-MS/MS and collision-induced dissociation-MS). On-line UV and MS data demonstrated the presence of methoxyflavone glycosides and aglycones in a total of seven compounds. Three naturally occurring flavones glycosides and two unusual erythro- and threo-diastereoisomeric flavolignan 7-O-glucosides were identified together with their aglycones.
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, Caixa Postal 780, 13560-970 São Carlos, SP, Brazil.
authors.forename: Renata a
authors.initials: Ra
authors.lastname: Colombo
authors.forename: Janete H
authors.initials: JH
authors.lastname: Yariwake
authors.forename: Emerson F
authors.initials: EF
authors.lastname: Queiroz
authors.forename: Karine
authors.initials: K
authors.lastname: Ndjoko
authors.forename: Kurt
authors.initials: K
authors.lastname: Hostettmann
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D047309:Flavones / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis; D006027:Glycosides / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D009862:Online Systems; D018515:Plant Leaves / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000737:chemistry; D031786:Saccharum / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D047309:Flavones; D005419:Flavonoids; D006027:Glycosides