Labeling of proteins and oligopeptides with luminescent lanthanide(III) chelates.
Synthesis of a building block that allows introduction of photoluminescent europium(III) and samarium(III) chelates to synthetic oligopeptides on solid phase using standard Fmoc chemistry is described. Upon completion of the oligopeptide synthesis, these conjugates were converted to the corresponding lanthanide(III) chelates by treatment with appropriate lanthanide(III) salt. Also synthesis of a new terpyridine-based europium(III) chelate designed for solution phase protein labeling is demonstrated.
Jaakkola
Lassi
L
PerkinElmer Life and Analytical Sciences, Turku Site, P.O. Box 10, FIN-20101 Turku, Finland.
Peuralahti
Jari
J
Hakala
Harri
H
Mukkala
Veli-Matti
VM
Hurskainen
Pertti
P
Hovinen
Jari
J
D002614:Chelating Agents / Q000737:chemistry*; D007202:Indicators and Reagents; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D015394:Molecular Structure; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry
D002614:Chelating Agents; D007202:Indicators and Reagents; D028581:Lanthanoid Series Elements; D049408:Luminescent Agents; D009842:Oligopeptides; D011506:Proteins; D012996:Solutions