title: Total internal reflection fluorescence microscopy in single molecule nanobioscience.
abstract: Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of biomolecules at the single-molecule level both in vitro and in living cells. This technique has opened a new era in bioscience, so-called single-molecule nanobioscience. In this review, we will summarize the theoretical basis of TIRFM, explain the instruments of TIRFM for single-molecules imaging, and discuss some novel applications of TIRFM to single-molecule nanobioscience.
authors.affiliation: Laboratory of Nanodevices, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan. wazawa@fbs.osaka-u.ac.jp
authors.forename: Tetsuichi
authors.initials: T
authors.lastname: Wazawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ueda
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*; D011506:Proteins / Q000378:metabolism* / Q000648:ultrastructure*; D012586:Science / Q000295:instrumentation / Q000379:methods
chemical_list: D011506:Proteins