Measurement of resiniferatoxin in serum samples by high-performance liquid chromatography.
A novel and simple method of extraction, separation, identification and quantification of resiniferatoxin (RTX) in serum samples is reported. Human serum and whole blood were treated with acetonitrile to denature proteins, such as orosomucoid, and the soluble fraction was passed through a reversed-phase C18 cartridge. RTX eluted from the cartridge was quantified by high-performance liquid chromatography (HPLC) using a reversed-phase C18 column. Reproducible recovery of RTX and tinyatoxin, an internal standard, from serum was achieved. Isocratic elution with 62% acetonitrile provided a suitable retention time without interfering peaks eluting near the analyte. Therefore, the procedure described provides a useful assay for determination of serum RTX pharmacokinetic parameters.
Mannes
Andrew J
AJ
DASS/NIH, Building 49, Room 1A08, 49 Convent Drive Bethesda, MD 20892, USA. amannes@nih.gov
Brown
Dorothy Cimino
DC
Keller
Jason
J
Cordes
Lauren
L
Eckenhoff
Roderic G
RG
Caudle
Robert M
RM
Iadarola
Michael J
MJ
Meng
Qing C
QC
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes / Q000097:blood* / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results
D004224:Diterpenes; C024353:resiniferatoxin