The late blight resistance locus Rpi-bib3 from Solanum bulbocastanum belongs to a major late blight R gene cluster on chromosome 4 of potato.
Late blight, caused by Phytophthora infestans, is one of the most devastating diseases in cultivated potato. Breeding of new potato cultivars with high levels of resistance to P. infestans is considered the most durable strategy for future potato cultivation. In this study, we report the identification of a new late-blight resistance (R) locus from the wild potato species Solanum bulbocastanum. Using several different approaches, a high-resolution genetic map of the new locus was generated, delimiting Rpi-blb3 to a 0.93 cM interval on chromosome 4. One amplification fragment length polymorphism marker was identified that cosegregated in 1,396 progeny plants of an intraspecific mapping population with Rpi-blb3. For comparative genomics purposes, markers linked to Rpi-blb3 were tested in mapping populations used to map the three other late-blight R loci Rpi-abpt, R2, and R2-like also to chromosome 4. Marker order and allelic conservation suggest that Rpi-blb3, Rpi-abpt, R2, and R2-like reside in the same R gene cluster on chromosome 4 and likely belong to the same gene family. Our findings provide novel insights in the evolution of R gene clusters conferring late-blight resistance in Solanum spp.
Park
Tae-Ho
TH
Graduate School Experimental Plant Sciences, Laboratory of Plant Breeding, Department of Plant Sciences, Wageningen University, Binnenhaven 5, 6709 PD, Wageningen, The Netherlands.
Gros
Jack
J
Sikkema
Anne
A
Vleeshouwers
Vivianne G A A
VG
Muskens
Marielle
M
Allefs
Sjefke
S
Jacobsen
Evert
E
Visser
Richard G F
RG
van der Vossen
Edwin A G
EA
D001483:Base Sequence; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D023281:Genomics; D005810:Multigene Family; D010838:Phytophthora / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D011110:Polymorphism, Genetic; D032322:Solanum / Q000235:genetics* / Q000382:microbiology*; D011198:Solanum tuberosum / Q000235:genetics* / Q000382:microbiology*; D013045:Species Specificity
D018744:DNA, Plant