[Isolation and purification of total tubeimosides from Bolbostemma paniculatum by macroporous resin].
OBJECTIVE
To select the optimum purification process of all tubeimosides from Bolbostemma paniculatum by macroporous resin.


METHODS
Static absorption test and deabsorption test were carried out to screen the best macroporous resin for all tubeimosides. The single factor test, orthogonal experimental designs and variance analysis were applied to optimize the manipulation parameters of macroporous resin.


RESULTS
The macroporous resin NKA possessed the strongest absorption ability to all tubeimosides among the 9 resins studied, in addition to an easy de-absorption property. The optimum absorption conditions were A2B3C1, namely concentration of feed 30 mg x mL(-1), diameter: height of the chromatography column 1:7, the weight ratio of raw material and resins 1:2.


CONCLUSIONS
The above experimental results can provide experimental basis for large-scale purification process of all tubeimosides.
Ma
Ting-jun
TJ
School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, China.
Tu
Peng-fei
PF
Lv
Fei-jie
FJ
D019662:Cucurbitaceae / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000302:isolation & purification*; D013678:Technology, Pharmaceutical / Q000379:methods*; D014315:Triterpenes / Q000302:isolation & purification*
D012117:Resins, Synthetic; D012503:Saponins; D014315:Triterpenes; C051084:tubeimoside I