Effects of modified low-density lipoproteins on human retinal pericyte survival.
According to a current paradigm cardiovascular diseases can be initiated by exposure of vascular cells to qualitatively modified low-density lipoproteins (LDL). Capillary leakage, an early feature of diabetic retinopathy, results in the exposure of retinal pericytes to modified LDL, including glycated (G-LDL) and heavily oxidized glycated LDL (HOG-LDL). We demonstrate here that modified LDL inhibits the proliferation and survival of cultured human retinal pericytes. Modified LDL also induced DNA fragmentation in bovine retinal pericytes. Overall, HOG-LDL produced a significantly higher extent of cytotoxicity and apoptosis in retinal pericytes. These results indicate that exposure of pericytes to HOG-LDL could be implicated in the development of diabetic retinopathy.
Song
Weiwei
W
Medicine Endocrinology, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, OK 73190, USA.
Barth
Jeremy L
JL
Lu
Kangmo
K
Yu
Yongxin
Y
Huang
Yan
Y
Gittinger
Cynthia K
CK
Argraves
W Scott
WS
Lyons
Timothy J
TJ
D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival; D053938:DNA Fragmentation / Q000187:drug effects; D017127:Glycation End Products, Advanced; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D020286:Pericytes / Q000166:cytology* / Q000187:drug effects; D012160:Retina / Q000166:cytology*; D000093844:Glycated Proteins
D017127:Glycation End Products, Advanced; D008077:Lipoproteins, LDL; C031797:glycated lipoproteins, LDL; D000093844:Glycated Proteins