Spylidone, a novel inhibitor of lipid droplet accumulation in mouse macrophages produced by Phoma sp. FKI-1840.
During our screening for microbial inhibitors of lipid droplet accumulation in macrophages, a new compound designated spylidone was isolated along with two structurally related known compounds, PF1052 and vermisporin, from the fermentation broth of Phoma sp. FKI-1840 by solvent extraction, silica gel column chromatography, ODS column chromatography and preparative HPLC. From the structure elucidation, spylidone has a spiro ring containing 2,4-pyrrolidinedione. Among the three compounds, only spylidone was found to inhibit lipid droplet accumulation in macrophages at 10 to approximately 50 microM without any cytotoxic effect.
Koyama
Nobuhiro
N
School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Nagahiro
Tomoko
T
Yamaguchi
Yuichi
Y
Ohshiro
Taichi
T
Masuma
Rokuro
R
Tomoda
Hiroshi
H
Omura
Satoshi
S
D000818:Animals; D001203:Ascomycota / Q000737:chemistry* / Q000145:classification; D001419:Bacteria / Q000187:drug effects; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005285:Fermentation; D005658:Fungi / Q000187:drug effects; D050356:Lipid Metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008826:Microbial Sensitivity Tests; D008968:Molecular Conformation; D010616:Phenanthrenes / Q000494:pharmacology*; D012988:Soil Microbiology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013141:Spiro Compounds / Q000494:pharmacology*
D010616:Phenanthrenes; D013141:Spiro Compounds; C502979:spylidone