title: Rv2358 and FurB: two transcriptional regulators from Mycobacterium tuberculosis which respond to zinc.
abstract: In a previous work, we demonstrated that the Mycobacterium tuberculosis Rv2358-furB operon is induced by zinc. In this study, the orthologous genes from Mycobacterium smegmatis mc(2)155 were inactivated and mutants analyzed. Rv2358 protein was purified and found to bind upstream of the Rv2358 gene. Binding was inhibited by Zn(2+) ions.
authors.affiliation: Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, via Ferrata 1, 27100 Pavia, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Canneva
authors.forename: Manuela
authors.initials: M
authors.lastname: Branzoni
authors.forename: Giovanna
authors.initials: G
authors.lastname: Riccardi
authors.forename: Roberta
authors.initials: R
authors.lastname: Provvedi
authors.forename: Anna
authors.initials: A
authors.lastname: Milano
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D017353:Gene Deletion; D008969:Molecular Sequence Data; D020102:Mycobacterium smegmatis / Q000235:genetics* / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism; D009876:Operon / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000502:physiology; D011485:Protein Binding / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors; D015032:Zinc