title: Development of a competitive immunoassay for the determination of N-(2-hydroxypropyl)valine adducts in human haemoglobin and its application in biological monitoring.
abstract: Propylene oxide (PO) is an important industrial compound and a directly acting mutagen. Human exposure to PO can be monitored by the determination of haemoglobin adducts. An immunoassay that quantifies the N-terminal adduct N-(2-hydroxypropyl)valine in whole haemoglobin was developed and its potential usefulness as a tool for biologically monitoring occupational exposure was demonstrated. Analytical reliability was confirmed in a comparative study with GC-MS (range 3.7-992 nmol g-1 haemoglobin (Hb), correlation coefficient 0.99, n=10). The assay has been configured as a competitive enzyme-linked immunosorbent assay to facilitate the rapid throughput of samples. The assay employs a whole blood matrix and has a working range of 2-250 pmol g-1 Hb. It does not appear to be affected by structurally similar metabolites and has been used to determine adducts in human blood samples. The first results in potentially exposed workers indicate the assay's high potential usefulness in routine occupational biomonitoring of exposure to PO.
authors.affiliation: AB Biomonitoring Ltd, Heath Park, Cardiff, UK. lball@abbiomonitoring.co.uk
authors.forename: L
authors.initials: L
authors.lastname: Ball
authors.forename: A
authors.initials: A
authors.lastname: Jones
authors.forename: P
authors.initials: P
authors.lastname: Boogaard
authors.forename: W
authors.initials: W
authors.lastname: Will
authors.forename: P
authors.initials: P
authors.lastname: Aston
mesh_terms: D000818:Animals; D015415:Biomarkers; D002855:Chromatography, Thin Layer; D004784:Environmental Monitoring; D004797:Enzyme-Linked Immunosorbent Assay; D004852:Epoxy Compounds / Q000009:adverse effects*; D008401:Gas Chromatography-Mass Spectrometry; D006433:Hemocyanins / Q000737:chemistry; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007118:Immunoassay; D009153:Mutagens / Q000009:adverse effects*; D016273:Occupational Exposure / Q000032:analysis*; D010455:Peptides / Q000138:chemical synthesis; D011817:Rabbits; D012709:Serum Albumin / Q000737:chemistry; D014633:Valine / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D015415:Biomarkers; D004852:Epoxy Compounds; D006454:Hemoglobins; D009153:Mutagens; D010455:Peptides; D012709:Serum Albumin; D006433:Hemocyanins; C057443:2-hydroxypropylvaline; D014633:Valine; C009068:propylene oxide