Development of a shear force scanning near-field fluorescence microscope for biological applications.
In this paper, a shear force scanning near-field fluorescence microscope combined with a confocal laser microspectrofluorometer is described. The shear force detection is realized based on a bimorph cantilever, which provides a very sensitive, reliable, and easy to use method to control the probe-sample distance during scanning. With the system, high-quality shear force imaging of various samples has been carried out. Furthermore, simultaneous shear force and near-field fluorescence imaging of biological cells has also been realized. As an example, we especially present the result on the distribution of P-glycoprotein in the plasma membrane of human small cell lung cancer cells, suggesting that the system would be a promising tool for biological applications.
Shang
G Y
GY
LMEN, (EA), UFR des Sciences, Université de Reims Champagne-Ardenne, 21 rue Clément Ader, 51685 Reims Cédex 2, France.
Qiao
W H
WH
Lei
F H
FH
Angiboust
J-F
JF
Troyon
M
M
Manfait
M
M
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism*; D018288:Carcinoma, Small Cell / Q000378:metabolism / Q000648:ultrastructure*; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000648:ultrastructure*; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation* / Q000379:methods*
D018435:ATP Binding Cassette Transporter, Subfamily B