Thrombospondins, metallo proteases and thrombospondin receptors messenger RNA and protein expression in different tumour sublines of the Dunning prostate cancer model.
Thrombospondin is a potent inhibitor of angiogenesis and might therefore be important in controlling tumour growth. TSP interacts with a number of proteases and receptors and in this way inhibits stimulation of angiogenesis. An earlier study showed that thrombospondin is expressed in benign prostatic hyperplasia (BPH) and high-grade prostatic intraepithelial neoplasia (PIN) but is absent in prostate cancer. The present study was therefore designed to evaluate the expression of thrombospondin 1 and 2 (TSP-1, TSP-2), TSP receptors CD36 and CD47, and matrix-metalloproteases 2 and 9 (MMP-, MMP-9) in a rat prostate cancer model. By using immunohistochemistry, Western blot, and real-time PCR the expression patterns of TSP-1, TSP-2, CD36, CD47, MMP-2, and MMP-9 were investigated in normal rat prostate tissue and five malignant Dunning sublines tissue. TSP-1 mRNA levels were decreased in all tumours compared with normal prostate. However, there was no difference in expression of TSP-2 and CD36 mRNA in these samples. MMP-2 was increased with malignancy, but no expression of MMP-9 was seen. The CD47 receptor did slightly increase with malignancy except for H3327. The results showed that thrombospondin is expressed in normal prostate but not in prostate tumours in a rat model. Simultaneously, MMP-2 expression increases with malignancy.
Vallbo
Christina
C
Institute of Surgical Sciences, Department of Urology, Göteborg University, Sweden. christina.vallbo@urology.gu.se
Damber
Jan-Erik
JE
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D018955:CD36 Antigens / Q000032:analysis* / Q000235:genetics; D051928:CD47 Antigen; D015815:Cell Adhesion Molecules / Q000032:analysis; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D015972:Gene Expression Regulation, Neoplastic; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D020782:Matrix Metalloproteinases / Q000032:analysis* / Q000235:genetics; D009368:Neoplasm Transplantation; D011467:Prostate / Q000166:cytology; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology; D019700:Thrombospondin 1 / Q000032:analysis; D019699:Thrombospondins / Q000032:analysis* / Q000235:genetics; D000099262:Thrombospondin 2
D015703:Antigens, CD; D018955:CD36 Antigens; D051928:CD47 Antigen; D015815:Cell Adhesion Molecules; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D020782:Matrix Metalloproteinases; D012333:RNA, Messenger; D019700:Thrombospondin 1; D019699:Thrombospondins; D000099262:Thrombospondin 2