title: The effect of polyclonal antibody on intracellular calcium increase induced by Mycoplasma hyopneumoniae in porcine tracheal cells.
abstract: An investigation was undertaken to assess whether polyclonal convalescent and hyperimmune sera obtained from pigs inhibit Mycoplasma hyopneumoniae induced increases in intracellular calcium [Ca2+](i) in ciliated porcine tracheal cells. Basal [Ca2+](i) in the tracheal cells was 97+/-13 nM (n=22 cells in four experiments) and after exposure to M. hyopneumoniae (300 micro g/mL or 10(11) CCU/mL), [Ca2+](i) increased by 246+/-56 nM within 100 s. After pre-treatment with hyperimmune or convalescent serum, M. hyopneumoniae increased [Ca2+](i) by 196+/-43 and 223+/-65 nM, respectively. It was found that neither hyperimmune nor convalescent serum significantly prevented the increase in [Ca2+](i) compared with M. hyopneumoniae alone. It was concluded that polyclonal antibodies produced by mycoplasma vaccination or exposure to the pathogen do not prevent M. hyopneumoniae-induced increase in [Ca2+](i).
authors.affiliation: Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Mi-Hyun
authors.initials: MH
authors.lastname: Hwang
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Lim
authors.forename: Hyo-In
authors.initials: HI
authors.lastname: Yun
authors.forename: Jong-Choon
authors.initials: JC
authors.lastname: Kim
authors.forename: Byeong-Yeal
authors.initials: BY
authors.lastname: Jung
authors.forename: Walter H
authors.initials: WH
authors.lastname: Hsu
authors.forename: Seung-Chun
authors.initials: SC
authors.lastname: Park
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D008856:Microscopy, Fluorescence; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000378:metabolism* / Q000382:microbiology; D013552:Swine; D014132:Trachea
chemical_list: D000907:Antibodies, Bacterial; D002118:Calcium