title: Cellular proliferation and migration following a controlled cortical impact in the mouse.
abstract: Neurogenesis following neural degeneration has been demonstrated in many models of disease and injury. The present study further examines the early proliferative and migratory response of the brain to a controlled cortical impact (CCI) model of traumatic brain injury. The CCI was centered over the forelimb sensorimotor cortex, unilaterally, in the adult mouse. To examine proliferation, bromo-deoxyuridine (BrdU) was injected i.p. immediately post-injury and on post-injury days 1, 2, and 3. To assess migration, we labeled SVZ cells with inert latex microspheres immediately post-injury. By combining microsphere labeling with BrdU, we determined if migrating cells had gone through the S-phase of the cell cycle after the lesion. In addition, we used a marker of neurogenesis and migration, doublecortin, to further characterize the response of the SVZ to the injury. Lastly, we determined whether subregions of the SVZ respond differentially to injury. The current study demonstrates that 3 days following CCI cellular proliferation is seen around the cortex, in the SVZ, corpus callosum, and subcortical areas anatomically connected to, but not directly damaged by the impact. It delineates that an increase in proliferation occurs in the dorsal-most aspect of the ipsilateral SVZ following impact. Lastly, it demonstrates that proliferating cells migrate from the SVZ to cortical and subcortical structures affected by the injury and that some of these cells are migrating neuroblasts.
authors.affiliation: Department of Biological Sciences, DePaul University, 2325 N. Clifton, Chicago, IL 60614, USA.
authors.forename: Shilpa
authors.initials: S
authors.lastname: Ramaswamy
authors.forename: Gwen E
authors.initials: GE
authors.lastname: Goings
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Soderstrom
authors.forename: Francis G
authors.initials: FG
authors.lastname: Szele
authors.forename: Dorothy A
authors.initials: DA
authors.lastname: Kozlowski
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000473:pathology* / Q000503:physiopathology*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D002540:Cerebral Cortex / Q000293:injuries / Q000473:pathology*; D004195:Disease Models, Animal; D000089622:Doublecortin Domain Proteins; D007839:Functional Laterality / Q000502:physiology; D020547:Lateral Ventricles / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008863:Microspheres; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D000089622:Doublecortin Domain Proteins; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D014446:Tyrosine 3-Monooxygenase; D001973:Bromodeoxyuridine