title: Differential expression of function-related antigens on blood monocytes in children with hemolytic uremic syndrome.
abstract: Monocytes (Mo) mediate central functions in inflammation and immunity. Different subpopulations of Mo with distinct phenotype and functional properties have been described. Here, we investigate the phenotype and function of peripheral Mo from children with hemolytic uremic syndrome (HUS). For this purpose, blood samples from patients in the acute period of HUS (HUS AP) were obtained on admission before dialysis and/or transfusion. The Mo phenotypic characterization was performed on whole blood by flow cytometry, and markers associated to biological functions were selected: CD14 accounting for lipopolysaccharide (LPS) responsiveness, CD11b for adhesion, Fc receptor for immunoglobulin G type I (FcgammaRI)/CD64 for phagocytosis and cytotoxicity, and human leukocyte antigen (HLA)-DR for antigen presentation. Some of these functions were also determined. Moreover, the percentage of CD14+ CD16+ Mo was evaluated. We found that the entire HUS AP Mo population exhibited reduced CD14, CD64, and CD11b expression and decreased LPS-induced tumor necrosis factor production and Fcgamma-dependent cytotoxicity. HUS AP showed an increased percentage of CD14+ CD16+ Mo with higher CD16 and lower CD14 levels compared with the same subset from healthy children. Moreover, the CD14++ CD16- Mo subpopulation of HUS AP had a decreased HLA-DR expression, which correlated with severity. In conclusion, the Mo population from HUS AP patients presents phenotypic and functional alterations. The contribution to the pathogenesis and the possible scenarios that led to these changes are discussed.
authors.affiliation: Division of Immunology of the Institute of Hematological Investigations, Academia Nacional de Medicina, Buenos Aires, Argentina. gfernandez@hematologia.anm.edu.ar
authors.forename: Gabriela C
authors.initials: GC
authors.lastname: Fernández
authors.forename: María V
authors.initials: MV
authors.lastname: Ramos
authors.forename: Sonia A
authors.initials: SA
authors.lastname: Gómez
authors.forename: Graciela I
authors.initials: GI
authors.lastname: Dran
authors.forename: Ramón
authors.initials: R
authors.lastname: Exeni
authors.forename: Marta
authors.initials: M
authors.lastname: Alduncín
authors.forename: Irene
authors.initials: I
authors.lastname: Grimoldi
authors.forename: Graciela
authors.initials: G
authors.lastname: Vallejo
authors.forename: Christian
authors.initials: C
authors.lastname: Elías-Costa
authors.forename: Martín A
authors.initials: MA
authors.lastname: Isturiz
authors.forename: Marina S
authors.initials: MS
authors.lastname: Palermo
mesh_terms: D000943:Antigens, Differentiation / Q000276:immunology*; D002452:Cell Count; D002675:Child, Preschool; D003602:Cytotoxicity, Immunologic; D005312:Fetal Blood / Q000276:immunology*; D005434:Flow Cytometry; D006463:Hemolytic-Uremic Syndrome / Q000175:diagnosis / Q000276:immunology* / Q000503:physiopathology*; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007223:Infant; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology*; D010641:Phenotype; D048069:Tumor Necrosis Factors / Q000276:immunology
chemical_list: D000943:Antigens, Differentiation; D007141:Immunoglobulin Fc Fragments; D008070:Lipopolysaccharides; D048069:Tumor Necrosis Factors