title: Viruses activate a genetically conserved cell death pathway in a unicellular organism.
abstract: Given the importance of apoptosis in the pathogenesis of virus infections in mammals, we investigated the possibility that unicellular organisms also respond to viral pathogens by activating programmed cell death. The M1 and M2 killer viruses of Saccharomyces cerevisiae encode pore-forming toxins that were assumed to kill uninfected yeast cells by a nonprogrammed assault. However, we found that yeast persistently infected with these killer viruses induce a programmed suicide pathway in uninfected (nonself) yeast. The M1 virus-encoded K1 toxin is primarily but not solely responsible for triggering the death pathway. Cell death is mediated by the mitochondrial fission factor Dnm1/Drp1, the K+ channel Tok1, and the yeast metacaspase Yca1/Mca1 encoded by the target cell and conserved in mammals. In contrast, cell death is inhibited by yeast Fis1, a pore-forming outer mitochondrial membrane protein. This virus-host relationship in yeast resembles that of pathogenic human viruses that persist in their infected host cells but trigger programmed death of uninfected cells.
authors.affiliation: Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Iva
authors.initials: I
authors.lastname: Ivanovska
authors.forename: J Marie
authors.initials: JM
authors.lastname: Hardwick
mesh_terms: D020169:Caspases / Q000502:physiology; D016923:Cell Death; D018920:Coculture Techniques; D055646:Killer Factors, Yeast; D008928:Mitochondria / Q000502:physiology; D009183:Mycotoxins / Q000502:physiology*; D012328:RNA Viruses / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000502:physiology* / Q000821:virology*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology; D015398:Signal Transduction
chemical_list: C089359:K1 killer toxin; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins; D020169:Caspases; C456272:MCA1 protein, S cerevisiae