title: Genetic analysis of Trichinella populations by 'cold' single-strand conformation polymorphism analysis.
abstract: A non-isotopic single-strand conformation polymorphism ('cold' SSCP) technique has been assessed for the analysis of sequence variability in the expansion segment 5 (ES5) of domain IV and the D3 domain of nuclear ribosomal DNA within and/or among isolates and individual muscle (first-stage) larvae representing all currently recognized species/genotypes of Trichinella. Data are consistent with the ability of cold SSCP to identify intra-specific as well as inter-specific variability among Trichinella genotypes. The cold SSCP approach should be applicable to a range of other genetic markers for comparative studies of Trichinella populations globally.
authors.affiliation: Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia. robinbg@unimelb.edu.au
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.forename: Youssef Abs
authors.initials: YA
authors.lastname: El-Osta
authors.forename: Dante S
authors.initials: DS
authors.lastname: Zarlenga
authors.forename: Edoardo
authors.initials: E
authors.lastname: Pozio
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D014234:Trichinella / Q000235:genetics*
chemical_list: D018509:DNA, Helminth; D012335:RNA, Ribosomal