Carbonylation of milk powder proteins as a consequence of processing conditions.
During industrial treatments, milk proteins could be oxidatively modified, thus leading to the formation of modified/oxidised amino acid residues. The apparition of such modified residues may contribute to the formation of new immunologically reactive structures. Some of these adducts could, in an advanced stage, lead to cross-linked protein species whose proteolytic susceptibility would be drastically decreased. Such protein species, that are resistant to digestion, could also constitute major food allergens. Therefore, these oxidative protein modifications tend to increase the natural allergenicity of milk proteins. For these reasons, monitoring milk protein oxidative modifications could be very useful regarding both product quality and allergenicity issues. In the present paper, we highlight, using different analytical approaches, the preferential carbonylation of beta-lactoglobulin (beta-Lg) during industrial treatments of milk. This result is particularly interesting since native beta-Lg represents one of the major milk allergens.
Fenaille
François
F
Nestlé Research Center, Nestec Ltd., Vers-Chez-Les-Blanc, CH-1000 Lausanne 26, Switzerland.
Parisod
Véronique
V
Tabet
Jean-Claude
JC
Guy
Philippe A
PA
D000447:Aldehydes / Q000737:chemistry; D000485:Allergens; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D002244:Carbon / Q000737:chemistry*; D004044:Dietary Proteins; D005511:Food Handling; D005512:Food Hypersensitivity; D006358:Hot Temperature; D007118:Immunoassay; D007782:Lactoglobulins / Q000737:chemistry; D013058:Mass Spectrometry; D008892:Milk / Q000737:chemistry; D016269:Milk Hypersensitivity / Q000175:diagnosis; D008894:Milk Proteins / Q000032:analysis* / Q000737:chemistry*; D008969:Molecular Sequence Data; D010100:Oxygen / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013053:Spectrophotometry; D014466:Ultraviolet Rays
D000447:Aldehydes; D000485:Allergens; D004044:Dietary Proteins; D007782:Lactoglobulins; D008894:Milk Proteins; D011506:Proteins; D002244:Carbon; C027576:4-hydroxy-2-nonenal; D010100:Oxygen