Characterization of an Escherichia coli sulfite oxidase homologue reveals the role of a conserved active site cysteine in assembly and function.
We report the biochemical and biophysical characterization of YedYZ, a sulfite oxidase homologue from Escherichia coli. YedY is a soluble catalytic subunit with a twin arginine leader sequence for export to the periplasm by the Tat translocation system. YedY is the only molybdoenzyme so far isolated from E. coli with the Mo-MPT form of the molybdenum cofactor. The electron paramagnetic resonance (EPR) signal of the YedY molybdenum is similar to that of known Mo-MPT containing enzymes, with the exception that only the Mo(IV) --> Mo(V) transition is observed, with a midpoint potential of 132 mV. YedZ is a membrane-intrinsic cytochrome b with six putative transmembrane helices. The single heme b of YedZ has a midpoint potential of -8 mV, determined by EPR spectroscopy of YedZ-enriched membrane preparations. YedY does not associate strongly with YedZ on the cytoplasmic membrane. However, mutation of the YedY active site Cys102 to Ser results in very efficient targeting of YedY to YedZ in the membrane, demonstrating a clear role for YedZ as the membrane anchor for YedY. Together, YedYZ comprise a well-conserved bacterial heme-molybdoenzyme found in a variety of bacteria that can be assigned to the sulfite oxidase class of enzyme.
Brokx
Stephen J
SJ
Membrane Protein Research Group, Department of Biochemistry, 474 Medical Sciences Building, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada.
Rothery
Richard A
RA
Zhang
Guijin
G
Ng
Derek P
DP
Weiner
Joel H
JH
D001665:Binding Sites / Q000235:genetics; D019295:Computational Biology; D017124:Conserved Sequence / Q000235:genetics; D003545:Cysteine / Q000737:chemistry* / Q000235:genetics; D004578:Electron Spin Resonance Spectroscopy; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D006639:Histidine / Q000737:chemistry; D016297:Mutagenesis, Site-Directed; D009842:Oligopeptides / Q000737:chemistry; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D011499:Protein Processing, Post-Translational; D012694:Serine / Q000235:genetics; D040681:Structural Homology, Protein; D013379:Substrate Specificity / Q000235:genetics; D014414:Tungsten / Q000737:chemistry
D029968:Escherichia coli Proteins; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D012694:Serine; D006639:Histidine; D010088:Oxidoreductases; C495992:YedY protein, E coli; D050862:Oxidoreductases Acting on Sulfur Group Donors; D003545:Cysteine; D014414:Tungsten