title: Establishment and characterization of a novel myxofibrosarcoma cell line.
abstract: We established a novel human myxofibrosarcoma cell line NMFH-1 and analyzed it with spectral karyotyping and comparative genomic hybridization (CGH). NMFH-1 cells are composed of two different types of cells, small, spindle-shaped mononuclear cells and bizarre multinucleated giant cells, which were maintained in vitro over 200 passages. Xenografted tumor showed typical features of myxofibrosarcoma, which included bizarre multinucleated giant cells. Cytogenetic analyses revealed complex abnormalities, including a t(17;22)(q2?2;q13), which has been found in dermatofibrosarcoma protuberans. Subsequent reverse-transcription polymerase chain reaction revealed that the cell line did not have the COL1A1-PDGFB gene fusion. Significant gains of the 1q12 approximately q23 and 8q13 approximately qter regions and loss of the 9p21 approximately pter and 13q12 regions often found in MFH were observed by CGH analysis. We investigated the origin of multinucleated giant cells in xenografted tumor through DNA in situ hybridization. In this system, the human-specific Alu sequence and the mouse L1 sequence were used as specific cell markers of identity. In situ hybridization revealed neoplastic proliferation of the multinucleated giant cells of human origin.
authors.affiliation: Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Niigata 951-8510, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawashima
authors.forename: Akira
authors.initials: A
authors.lastname: Ogose
authors.forename: Wenguang
authors.initials: W
authors.lastname: Gu
authors.forename: Jun
authors.initials: J
authors.lastname: Nishio
authors.forename: Naoko
authors.initials: N
authors.lastname: Kudo
authors.forename: Naoki
authors.initials: N
authors.lastname: Kondo
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Hotta
authors.forename: Hajime
authors.initials: H
authors.lastname: Umezu
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tohyama
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Nishijima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iwasaki
authors.forename: Naoto
authors.initials: N
authors.lastname: Endo
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D049109:Cell Proliferation; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D024042:Collagen Type I / Q000502:physiology; D000090602:Collagen Type I, alpha 1 Chain; D018223:Dermatofibrosarcoma / Q000235:genetics; D005260:Female; D005354:Fibrosarcoma / Q000145:classification / Q000235:genetics* / Q000473:pathology; D015726:Giant Cells / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D009693:Nucleic Acid Hybridization; D015514:Oncogene Proteins, Fusion / Q000502:physiology*; D020574:Proto-Oncogene Proteins c-sis / Q000502:physiology; D012878:Skin Neoplasms / Q000235:genetics; D032681:Spectral Karyotyping; D014178:Translocation, Genetic; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D015514:Oncogene Proteins, Fusion; D020574:Proto-Oncogene Proteins c-sis