title: Co-localization of PARP-1 and lamin B in the nuclear architecture: a halo-fluorescence- and confocal-microscopy study.
abstract: A functional interaction between poly(ADP-ribose) polymerase-1 (PARP-1) and lamin B has recently been proposed by nuclear fractionation, crosslinking, and immunoprecipitation experiments. Here we use fluorescence microscopy to verify and extend these findings. We analyze nuclear halo preparations by fluorescence in situ immuno staining (FISIS), which shares attributes with traditional nuclear fractionation techniques, and by confocal laser scanning microscopy (CLSM). The results agree in that a major part of the enzyme co-localizes with lamin B under physiological conditions, where PARP-1 only has basal activity. After DNA damage and the associated activation of PARP-1, and during the subsequent entry into apoptosis, dramatic changes occur: a gradual release of the enzyme from the lamina, accompanied by its accumulation in nucleoli. Our observations are in line with biochemical evidence for lamin B-PARP-1 interactions under physiological conditions and suggest ways by which these interactions are modified to support PARP-functions in damage and its fate in apoptosis.
authors.affiliation: Molecular Biology Laboratory, Institute for Biological Research, Despot Sephen Blvd. 142, 11060 Belgrade, Serbia and Montenegro.
authors.forename: Melita
authors.initials: M
authors.lastname: Vidaković
authors.forename: Mario
authors.initials: M
authors.lastname: Koester
authors.forename: Sandra
authors.initials: S
authors.lastname: Goetze
authors.forename: Silke
authors.initials: S
authors.lastname: Winkelmann
authors.forename: Martin
authors.initials: M
authors.lastname: Klar
authors.forename: Goran
authors.initials: G
authors.lastname: Poznanović
authors.forename: Juergen
authors.initials: J
authors.lastname: Bode
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure*; D005720:Gamma Rays; D007150:Immunohistochemistry / Q000379:methods; D034921:Lamin Type B / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*
chemical_list: D034921:Lamin Type B; C499321:Parp1 protein, mouse; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases