Clonal T cells of pure red-cell aplasia.
This study detected clonal T cells in patients with acquired pure red-cell aplasia (PRCA) by Southern blotting and polymerase chain reaction (PCR). Twenty-nine adult patients with acquired PRCA were enrolled in this study. Seventeen patients had primary acquired PRCA, while 12 patients had the secondary form. Twenty-two of 29 (76%) patients demonstrated TCR rearrangement by at least one method. We divided the patients into three groups depending on T-cell clonality. The CD4/8 ratio of patients who were positive on Southern blotting was significantly lower than that of other groups. Except for the CD4/8 ratio, other laboratory findings did not significantly differ among the three groups. The CD4/8 ratio should be a useful surrogate marker to detect T-cell clonality.
Masuda
Michihiko
M
Department of Hematology, Tokyo Women's Medical University, Tokyo, Japan. masuda@dh.twmu.ac.jp
Teramura
Masanao
M
Matsuda
Akira
A
Bessho
Masami
M
Shimamoto
Takashi
T
Ohyashiki
Kazuma
K
Omine
Mitsuhiro
M
Motoji
Toshiko
T
Mizoguchi
Hideaki
H
D000328:Adult; D015139:Blotting, Southern; D002999:Clone Cells; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D016133:Polymerase Chain Reaction; D012010:Red-Cell Aplasia, Pure / Q000150:complications / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*