title: Biocompatibility in vitro tests of mineral trioxide aggregate and regular and white Portland cements.
abstract: Mineral trioxide aggregate (MTA) and Portland cement are being used in dentistry as root end-filling materials. However, biocompatibility data concerning genotoxicity and cytotoxicity are needed for complete risk assessment of these compounds. In the present study, genotoxic and cytotoxic effects of MTA and Portland cements were evaluated in vitro using the alkaline single cell gel (comet) assay and trypan blue exclusion test, respectively, on mouse lymphoma cells. The results demonstrated that the single cell gel (comet) assay failed to detect DNA damage after a treatment of cells by MTA and Portland cements for concentrations up to 1000 microg/ml. Similarly, results showed that none of the compounds tested were cytotoxic. Taken together, these results seem to indicate that MTA and Portland cements are not genotoxins and do not induce cellular death.
authors.affiliation: Center for Genotoxins and Carcinogens Evaluation (TOXICAN), Department of Pathology, Botucatu Medical School, UNESP, SP Brazil. ak92@hotmail.com
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
authors.forename: Marco Antonio Hungaro
authors.initials: MA
authors.lastname: Duarte
authors.forename: Mariza Akemi
authors.initials: MA
authors.lastname: Matsumoto
authors.forename: Mariangela Esther Alencar
authors.initials: ME
authors.lastname: Marques
authors.forename: Daisy Maria Favero
authors.initials: DM
authors.lastname: Salvadori
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D017610:Calcium Compounds / Q000633:toxicity; D045744:Cell Line, Tumor / Q000187:drug effects; D004396:Coloring Agents; D020552:Comet Assay; D004249:DNA Damage; D003738:Dental Cements / Q000633:toxicity; D004338:Drug Combinations; D051379:Mice; D010087:Oxides / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity; D018709:Statistics, Nonparametric; D014343:Trypan Blue
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004396:Coloring Agents; D003738:Dental Cements; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C472247:accelerated Portland cement; C086631:mineral trioxide aggregate; D014343:Trypan Blue