Effect of neutrophil depletion on gelatinase expression, edema formation and hemorrhagic transformation after focal ischemic stroke.
BACKGROUND
While gelatinase (MMP-2 and -9) activity is increased after focal ischemia/reperfusion injury in the brain, the relative contribution of neutrophils to the MMP activity and to the development of hemorrhagic transformation remains unknown.


RESULTS
Anti-PMN treatment caused successful depletion of neutrophils in treated animals. There was no difference in either infarct volume or hemorrhage between control and PMN depleted animals. While there were significant increases in gelatinase (MMP-2 and MMP-9) expression and activity and edema formation associated with ischemia, neutrophil depletion failed to cause any change.


CONCLUSIONS
The main finding of this study is that, in the absence of circulating neutrophils, MMP-2 and MMP-9 expression and activity are still up-regulated following focal cerebral ischemia. Additionally, neutrophil depletion had no influence on indicators of ischemic brain damage including edema, hemorrhage, and infarct size. These findings indicate that, at least acutely, neutrophils are not a significant contributor of gelatinase activity associated with acute neurovascular damage after stroke.
Harris
Alex K
AK
Program in Clinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, Georgia, USA. aharris@mail.mcg.edu
Ergul
Adviye
A
Kozak
Anna
A
Machado
Livia S
LS
Johnson
Maribeth H
MH
Fagan
Susan C
SC
D000818:Animals; D001929:Brain Edema / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D002545:Brain Ischemia / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D002452:Cell Count / Q000379:methods; D018093:Gelatinases / Q000096:biosynthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D020300:Intracranial Hemorrhages / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D008297:Male; D009503:Neutropenia / Q000150:complications / Q000201:enzymology*; D009504:Neutrophils / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000201:enzymology* / Q000235:genetics / Q000473:pathology
D018093:Gelatinases