title: Impact of inspired substance concentrations on the results of breath analysis in mechanically ventilated patients.
abstract: A well-defined relationship has to exist between substance concentrations in blood and in breath if blood-borne volatile organic compounds (VOCs) are to be used as breath markers of disease or health. In this study, the impact of inspired substances on this relationship was investigated systematically. VOCs were determined in inspired and expired air and in arterial and mixed venous blood of 46 mechanically ventilated patients by means of SPME, GC/MS. Mean inspired concentrations were 25% of expired concentrations for pentane, 7.5% for acetone, 0.7% for isoprene and 0.4% for isoflurane. Only if inspired concentrations were <5% did substance disappearance rates from blood and exhalation rates correlate well. Exhaled substance concentrations depended on venous and inspired concentrations. Patients with sepsis had higher n-pentane and lower acetone concentrations in mixed venous blood than patients without sepsis (2.27 (0.37-8.70) versus 0.65 (0.33-1.48) nmol L-1 and 69 (22-99) versus 18 (6.7-56) micromol L-1). n-Pentane and acetone concentrations in breath showed no differences between the patient groups, regardless whether or not expired concentrations were corrected for inspired concentrations. In mechanically ventilated patients, concentration profiles of volatile substances in breath may considerably deviate from profiles in blood depending on the relative amount of inspired concentrations. A simple correction for inspired substance concentrations was not possible. Hence, substances having inspired concentrations>5% of expired concentrations should not be used as breath markers in these patients without knowledge of concentrations in blood and breath.
authors.affiliation: Department of Anaesthesiology and Intensive Care, University of Rostock, Rostock, Germany. jochen.schubert@med.uni-rostock.de
authors.forename: J K
authors.initials: JK
authors.lastname: Schubert
authors.forename: W
authors.initials: W
authors.lastname: Miekisch
authors.forename: T
authors.initials: T
authors.lastname: Birken
authors.forename: K
authors.initials: K
authors.lastname: Geiger
authors.forename: G F E
authors.initials: GF
authors.lastname: Nöldge-Schomburg
mesh_terms: D000096:Acetone / Q000032:analysis; D000328:Adult; D000368:Aged; D000465:Algorithms; D015415:Biomarkers; D001944:Breath Tests; D002070:Butadienes / Q000032:analysis; D002245:Carbon Dioxide / Q000032:analysis; D005260:Female; D045782:Hemiterpenes / Q000032:analysis; D006801:Humans; D019570:Inhalation Exposure / Q000032:analysis*; D007530:Isoflurane / Q000032:analysis; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010420:Pentanes / Q000032:analysis; D012044:Regression Analysis; D012121:Respiration, Artificial; D018805:Sepsis / Q000378:metabolism; D012997:Solvents / Q000032:analysis
chemical_list: D015415:Biomarkers; D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; D012997:Solvents; C005059:isoprene; D000096:Acetone; D002245:Carbon Dioxide; C033353:pentane; D007530:Isoflurane