title: Oral administration of Bifidobacterium bifidum G9-1 suppresses total and antigen specific immunoglobulin E production in mice.
abstract: Recent studies have suggested that oral bacteriotherapy with probiotics might be useful in the management of allergic diseases. We investigated the effect of oral administration of Bifidobacterium bifidum G9-1 (BBG9-1) on immunoglobulin (Ig) E production in BALB/c mice. Live BBG9-1 was orally administered to mice for 2 weeks from 1 week before ovalbumin (OVA)-immunization. The treatment of BBG9-1 significantly reduced serum total IgE level. In addition, BBG9-1 significantly and largely reduced the serum level of OVA-specific IgE without lowering of the specific IgG1 and increasing of the specific IgG2a. We also examined T helper type (Th) 1 and Th2 cytokine production from OVA-immunized splenocytes by restimulation with OVA in vitro. Productions of interferon (IFN)-gamma, interleukin (IL)-4 and IL-5 from the splenocytes of mice given BBG9-1 were weaker than those of control mice. We conclude that oral administration of BBG9-1 selectively and powerfully suppresses total and antigen specific IgE production in mice. It is suggested that BBG9-1 is useful for the prophylactic treatment in IgE-dependent allergic diseases.
authors.affiliation: Research & Development Department, Biofermin Pharmaceutical Co. Ltd., 7-3-4 Higashi-nachi, Ibukidai, Nishi-ku, Kobe 651-2242, Japan. kenkyu@biofermin.co.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohno
authors.forename: Satoru
authors.initials: S
authors.lastname: Tsunemine
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Isa
authors.forename: Masaki
authors.initials: M
authors.lastname: Shimakawa
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamura
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001644:Bifidobacterium; D004797:Enzyme-Linked Immunosorbent Assay; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000097:blood; D013154:Spleen / Q000166:cytology / Q000378:metabolism
chemical_list: D007378:Interleukins; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin