[Construction of monovalent and compound nucleic acid vaccines against Toxoplasma gondii with gene encoding p30].
OBJECTIVE
To construct a monovalent gene vaccine pcDNA3.1-p30 and a compound gene vaccine pcDNA3.1-p30-ROP2 and assess the protective effect of the two vaccines against Toxoplasma gondii.


METHODS
The sequences encoding p30 and ROP2 were amplified from the genomic DNA of T. gondii RH strain by polymerase chain reaction (PCR) and inserted into eukaryotic vector pcDNA3.1 to construct pcDNA3.1-p30 and pcDNA3.1-p30-ROP2. Mice were injected with the recombinant plasmid to observe the immunoprotectivity of the nucleic acid vaccine by using ELISA for detection of total IgG and observing the survival time after tachyzoites challenge.


RESULTS
The recombinant plasmids pcDNA3.1-p30 and pcDNA3.1-p30-ROP2 were constructed. Mice in pcDNA3.1-p30-ROP2 group showed higher IgG (P < 0.05) and survived longer than those in pcDNA3.1-p30 group (P < 0.01) after challenged with T. gondii.


CONCLUSIONS
Compound vaccine of genes from different stages of T. gondii elicits stronger immunoprotectivity in mice than a single gene vaccine.
Yang
Ting-ting
TT
Department of Parasitology, Medical College of Shandong University, Jinan 250012, China.
He
Shen-yi
SY
Jiang
Hua
H
Gu
Qin-min
QM
Cong
Hua
H
Zhou
Huai-yu
HY
Zhang
Jia-qin
JQ
Li
Ying
Y
Zhao
Qun-li
QL
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D016054:DNA, Protozoan / Q000235:genetics; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D007074:Immunoglobulin G; D008565:Membrane Proteins; D015800:Protozoan Proteins; C090555:ROP 2 protein, Toxoplasma gondii; C047168:SAG1 antigen, Toxoplasma; D019444:Vaccines, DNA