title: Flow cytometric analysis of CD55 and CD59 expression on blood cells in paroxysmal nocturnal haemoglobinuria.
abstract: PNH is a rare clonal disorder of hematopoietic stem cells, therefore all blood cells lineages are involved. The main feature is an increased sensitivity of erythrocytes to complement-mediated cell lysis due to deficiency of membrane-bound GPI (glycosylphosphatidylinositol)-anchored proteins which normally function as inhibitors of reactive hemolysis. In the present study, we performed flow cytometric analysis using monoclonal antibodies against CD55 and CD59 for the detection of PNH-type clone in the blood of 50 patients (28 females and 22 males, age range 7-67 yrs). In one patient only we found a large population (95%) of granulocytes with decreased expression of both CD55 and CD59 molecules (type I PNH) and in two others with partial loss of CD55 expression (type II PNH). The expression was determined chiefly on granulocytes which in the control group showed reliable and high expression of CD55 and CD59.
authors.affiliation: Deptartment of Clinical Immunology, University of Medical Sciences, Poznań, Poland. gdwrck@wp.pl
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Dworacki
authors.forename: Jan
authors.initials: J
authors.lastname: Sikora
authors.forename: Ewa
authors.initials: E
authors.lastname: Mizera-Nyczak
authors.forename: Magdalena
authors.initials: M
authors.lastname: Trybus
authors.forename: Iwona
authors.initials: I
authors.lastname: Mozer-Lisewska
authors.forename: Anna
authors.initials: A
authors.lastname: Czyz
authors.forename: Jan
authors.initials: J
authors.lastname: Zeromski
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018958:CD55 Antigens / Q000097:blood*; D018957:CD59 Antigens / Q000097:blood*; D002648:Child; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood / Q000276:immunology*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology
chemical_list: D018958:CD55 Antigens; D018957:CD59 Antigens; D008562:Membrane Glycoproteins