title: The association between eye color and corneal sensitivity measured using a Belmonte esthesiometer.
abstract: OBJECTIVE
  The purpose of this study is to determine the association between corneal sensitivity measured using a pneumatic esthesiometer and eye color quantified objectively.
  
  
  METHODS
  Twenty subjects had ocular surface sensitivity measured using a Belmonte esthesiometer. An ascending method of limits followed by the method of constant stimuli were used to estimate 1) cold detection thresholds, 2) discomfort detection thresholds (both using pneumatic stimuli at 20 degrees C, 3) mechanical detection thresholds using pneumatic stimuli at 50 degrees C (ocular surface temperature approximately 33 degrees C), and 4) percent CO2 chemical detection thresholds using 50 degrees C pneumatic stimuli at flow rates set at half of each subject's pneumatic detection threshold (therefore detected by the chemical content and not the mechanical content). Eye color was estimated 1) clinically by two observers ranking the color (light to dark) of digital images of each subject's iris, 2) photometrically by measuring iris luminance, and 3) using chromaticity obtained from a Photo Research 650 spectroradiometer with controlled illumination. Correlation and linear and nonlinear regression analyses were used to examine relationships between variables.
  
  
  RESULTS
  There were no associations between eye color (determined clinically or objectively) for mechanical and chemical detection thresholds (best r = 0.15, all p > 0.05). There was a significant linear association between 20 degrees detection thresholds and eye color (r = 0.39), which was substantially improved with a two-line function (part level and part increasing linearly, r = 0.65).
  
  
  CONCLUSIONS
  We were generally unable to demonstrate the relationship between eye color and sensitivity reported previously using a Cochet-Bonnet esthesiometer. However, for a subset of subjects with palest irises, there appears to be a linear association between eye color and sensitivity to cooling stimuli.
authors.affiliation: University of Waterloo, School of Optometry, Waterloo, Ontario, Canada.
authors.forename: Laura
authors.initials: L
authors.lastname: Henderson
authors.forename: David
authors.initials: D
authors.lastname: Bond
authors.forename: Trefford
authors.initials: T
authors.lastname: Simpson
mesh_terms: D000328:Adult; D003315:Cornea / Q000502:physiology*; D003941:Diagnostic Techniques, Ophthalmological; D005127:Eye Color / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D012677:Sensation / Q000502:physiology; D012684:Sensory Thresholds