title: Effect of aerobic exercise training on mtDNA deletion in soleus muscle of trained and untrained Wistar rats.
abstract: BACKGROUND
  According to the theory of mitochondrial aging, oxidative stress plays a major role in aging and age related degenerative diseases. Since oxygen consumption and reactive oxygen species rate increase during aerobic exercise, we hypothesised that heavy aerobic training could lead to enhanced mitochondrial DNA (mtDNA) deletion in postmitotic tissues, leading in turn to premature aging and degenerative diseases.
  
  
  METHODS
  Sixty adult male 2 month old Wistar14848 rats were divided into six equal groups. Two groups were trained for 3 months by running on a treadmill (5 days/week, incline 6 degrees; group 1: 40 m/min, 20 min/day; group 2: 20 m/min, 40 min/day), while two sedentary groups participated in aerobic exercise only at the end of the study (incline 6 degrees; group 3: 40 m/min; group 4: 20 m/min). To control for physical and physiological parameters, two groups of untrained animals were killed at the beginning (group 6) and end (group 5) of the study. Expand long PCR was used to investigate mtDNA deletion in soleus muscle and a sequencing method was used to confirm the mtDNA deletion break point.
  
  
  RESULTS
  Our results did not show any mtDNA deletion in untrained rats or in those that underwent moderate training (group 2) We only found mtDNA deletion (4.6 kb) in the soleus muscle of heavily trained rats (group 1).
  
  
  CONCLUSIONS
  These results demonstrate that one session of aerobic exercise does not cause mtDNA deletion in skeletal muscle. The difference in results between heavy and moderate aerobic training may be due to low work rate or up-regulation of inducible antioxidant systems in moderate training.
authors.affiliation: Tabriz University, 29 Bahman Street, Tabriz 98, Iran. ajafari@tabrizu.ac.ir
authors.forename: A
authors.initials: A
authors.lastname: Jafari
authors.forename: M A
authors.initials: MA
authors.lastname: Hosseinpourfaizi
authors.forename: M
authors.initials: M
authors.lastname: Houshmand
authors.forename: A A
authors.initials: AA
authors.lastname: Ravasi
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D017353:Gene Deletion; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species