Immunohistological study for estrogenic activities of nitrophenols in diesel exhaust particles.
We recently isolated 3-methyl-4-nitrophenol (4-nitro-m-cresol; PNMC) and 4-nitro-3-phenylphenol (PNMPP) from diesel exhaust particles (DEP) and identified them as vasodilators and xenoestrogens. The estrogenic activity of PNMC and PNMPP was further examined by using immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in uterine luminal epithelium of ovariectomized 25-d-old immature female rats injected with PNMC and PNMPP subcutaneously for 2 d. Significant increases were observed in uterine luminal epithelium in PCNA positive cells of animals receiving 10 and 100 mg/kg PNMC and 0.1 mg/kg PNMPP compared with controls. These results clearly show the estrogenic activity of PNMC and PNMPP by cell proliferation on the uterine luminal epithelium.
Furuta
Chie
C
Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu Univ, Gifu, Japan.
Li
ChunMei
C
Taneda
Shinji
S
Suzuki
Akira K
AK
Kamata
Kazuyuki
K
Watanabe
Gen
G
Taya
Kazuyoshi
K
D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry / Q000633:toxicity; D003408:Cresols / Q000737:chemistry / Q000633:toxicity; D004847:Epithelial Cells / Q000473:pathology; D004968:Estrogens, Non-Steroidal; D005260:Female; D007150:Immunohistochemistry; D009596:Nitrophenols / Q000737:chemistry / Q000633:toxicity*; D010052:Ovariectomy; D018809:Proliferating Cell Nuclear Antigen / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014599:Uterus / Q000473:pathology; D001335:Vehicle Emissions / Q000032:analysis / Q000633:toxicity*
C494060:4-nitro-3-phenylphenol; D001713:Biphenyl Compounds; D003408:Cresols; D004968:Estrogens, Non-Steroidal; D009596:Nitrophenols; D018809:Proliferating Cell Nuclear Antigen; D001335:Vehicle Emissions; C008636:4-nitro-3-cresol