title: [The relationship between expression of fas mRNA and apoptosis after traumatic brain injury in rats and the role of GM-1 in protecting the brain].
abstract: OBJECTIVE
  To explore the mechanism of apoptosis after traumatic brain injury (TBI) in rats and elucidate the role of GM-1 by detecting the expression of Fas mRNA and apoptosis in hippocampi.
  
  
  METHODS
  After creating the model of Marmarou cranio-cerebral trauma and offering GM-1 therapy, we observed the expression of Fas mRNA and apoptotic cell death using in situ hybridization and terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL) technique.
  
  
  RESULTS
  Increased expression of Fas mRNA and increased apoptotic cells in hippocampi after TBI were observed. GM-1 could decrease the expression of Fas mRNA and apoptotic cell death.
  
  
  CONCLUSIONS
  The increased expression of Fas mRNA may be a noteworthy cause of apoptotic cell death after traumatic brain injury. GM-1 may play a protective role by way of decreasing the expression of Fas mRNA and apoptotic cell death.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Northern China Coal Medical College, Tangshan 063000, China.
authors.forename: Qing-jun
authors.initials: QJ
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Jing-xia
authors.initials: JX
authors.lastname: Zhao
authors.forename: Jian-min
authors.initials: JM
authors.lastname: Li
authors.forename: Ai-jun
authors.initials: AJ
authors.lastname: Fu
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001930:Brain Injuries / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000494:pharmacology; D006624:Hippocampus / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018696:Neuroprotective Agents; D012333:RNA, Messenger; D019014:fas Receptor; C502266:ganglioside GM1alpha; D005677:G(M1) Ganglioside