Amino acid sequences of ferredoxins from Atropa belladonna and Hyoscyamus niger: their similarities to those in other tropane-alkaloid-containing plants.
The complete amino acid sequences of [2Fe-2S] ferredoxin from Atropa belladonna and Hyoscyamus niger have been determined by automated Edman degradation of the entire S-carboxymethylcysteinyl proteins and of the peptides obtained by enzymatic digestion. These two ferredoxins exhibited 1-8 differences in their amino acid sequences compared to those of other tropane-alkaloid-containing plants (Scopolia japonica, Datura stramonium, D. metel, and D. arborea), and only 1 or 4 differences compared to S. japonica and D. arborea. In contrast, 9-23 differences were observed among the other solanaceous ferredoxins. This suggests that tropane-alkaloid-containing plants are closely related taxonomically.
Mino
Yoshiki
Y
Department of Environment Analysis, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. mino@gly.oups.ac.jp
Yukita
Mayumi
M
Hiratsuka
Nobuhiro
N
Wariishi
Hiroyuki
H
D000595:Amino Acid Sequence; D001532:Atropa belladonna / Q000737:chemistry* / Q000145:classification; D005288:Ferredoxins / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D032304:Hyoscyamus / Q000737:chemistry* / Q000145:classification; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
D005288:Ferredoxins