title: On-line hyphenation of quantum cascade laser and capillary electrophoresis.
abstract: We report the first successful hyphenation of a Fabry Pérot quantum cascade (QC) laser to a capillary electrophoresis system. This involved use of a dedicated IR-transparent flow cell, made of CaF2, constructed by means of SU-8 based lithography and low temperature wafer bonding techniques. Adenosine, guanosine, xanthosine and adenosine-5'-monophosphate were separated in a borate-containing separation electrolyte (10 mM, pH 9.3). Functional group (carbohydrate) detection was accomplished by use of the 1080 cm(-1) emission line of the available QC-laser. The assessable optical path length could be increased, from the normally available 10-15 microm in CE-FTIR analyses, to 60 microm using this powerful mid-infrared laser and aqueous solutions.
authors.affiliation: Department of Analytical Chemistry, Stockholm University, SE-106 91 Stockholm, Sweden.
authors.forename: Malin
authors.initials: M
authors.lastname: Kölhed
authors.forename: Stefan
authors.initials: S
authors.lastname: Schaden
authors.forename: Bo
authors.initials: B
authors.lastname: Karlberg
authors.forename: Bernhard
authors.initials: B
authors.lastname: Lendl
mesh_terms: D000241:Adenosine / Q000302:isolation & purification; D000249:Adenosine Monophosphate / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D006151:Guanosine / Q000302:isolation & purification; D007834:Lasers; D009862:Online Systems; D011789:Quantum Theory; D012263:Ribonucleosides / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D012263:Ribonucleosides; D006151:Guanosine; D000249:Adenosine Monophosphate; D000241:Adenosine