Cross-clade conservation of HIV type 1 Nef immunodominant regions recognized by CD8+ T cells of HIV type 1 CRF02_AG-infected Ivorian (West Africa).
Most HIV vaccine trials in the world are conducted with clade B while most circulating viral strains in Africa are non-B subtypes. We determined whether CD8+ T cells from HIV-1 intersubtype CRF02_AG-infected Ivorian individuals were able to recognize clade B epitopes. CD8+ T cell responses of nine HIV-1 intersubtype CRF02_AG-infected Ivorian patients and nine HIV-1 subtype B-infected French patients were studied using pools of HIV-1 clade B peptides (110 well-defined HIV CD8+ T cell epitopes) in an ELISPOT IFN-gamma assay. There was no difference in the number of recognized peptide pools between Ivorian and French cohorts (mean of four pools in both cases). Ivorian individuals had generated CD8+ T cell responses cross-reactive against HIV-1 subtype B and some individual peptides had been identified. Furthermore, sequence analysis of nef HIV genes of the Ivorian patients and nef cloning in two patients revealed very few variations between HIV- 1 intersubtype CRF02_AG and subtype B in nef immunodominant regions included in HIV clade B lipopeptide vaccines, currently tested in France.
Inwoley
André
A
CeDReS/PAC-CI, CHU Treichville, Abidjan, Côte d'Ivoire.
Recordon-Pinson
Patricia
P
Dupuis
Marion
M
Gaston
Jessintha
J
Genête
Mathieu
M
Minga
Albert
A
Letourneur
Franck
F
Rouet
François
F
Choppin
Jeannine
J
Fleury
Hervé
H
Guillet
Jean-Gérard
JG
Andrieu
Muriel
M
D000595:Amino Acid Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015331:Cohort Studies; D007560:Cote d'Ivoire; D015693:Gene Products, nef / Q000737:chemistry* / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D054311:nef Gene Products, Human Immunodeficiency Virus
D015693:Gene Products, nef; D016056:Immunodominant Epitopes; D054311:nef Gene Products, Human Immunodeficiency Virus