title: Long-term survival of bone marrow-derived retinal nerve cells in the retina.
abstract: Recently, we have demonstrated that bone marrow stem cells can differentiate into retinal nerve cells. In the present study, we show a new and efficient strategy for transplanting bone marrow stem cells into the retina. When bone marrow stem cells were injected into the vitreous cavity of untreated eyes, only very few cells were found in the retina 2 weeks after injection. In contrast, when laser photocoagulation was performed just before the injection of bone marrow stem cells, a large number of the injected cells survived 2 weeks after injection and the cells expressed neural cell-specific or retinal nerve cell-specific antigens. Moreover, we still detected bone marrow stem cell-derived retinal nerve cells in the retina 1 year after injection in the retina.
authors.affiliation: First Department of Pathology, Kansai Medical University, Moriguchi city, Osaka, Japan.
authors.forename: Keizo
authors.initials: K
authors.lastname: Minamino
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Adachi
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Yamada
authors.forename: Akiko
authors.initials: A
authors.lastname: Higuchi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Iwasaki
authors.forename: Keiji
authors.initials: K
authors.lastname: Nakano
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Koike
authors.forename: Hiromi
authors.initials: H
authors.lastname: Mukaide
authors.forename: Naoko
authors.initials: N
authors.lastname: Kiriyama
authors.forename: Akio
authors.initials: A
authors.lastname: Shigematsu
authors.forename: Miyo
authors.initials: M
authors.lastname: Matsumura
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002452:Cell Count / Q000379:methods; D006085:Graft Survival / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008028:Light Coagulation / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000502:physiology*; D012160:Retina / Q000166:cytology*; D012243:Rhodopsin / Q000378:metabolism; D013234:Stem Cells / Q000502:physiology*; D013997:Time Factors
chemical_list: D007381:Intermediate Filament Proteins; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D049452:Green Fluorescent Proteins; D012243:Rhodopsin