Development and validation of a method for the quantitative determination of aflatoxin contaminants in Maytenus ilicifolia by HPLC with fluorescence detection.
A method for the quantification of aflatoxins B1, G1, B2 and G2 in the medicinal herb Maytenus ilicifolia was developed and validated. The method used immunoaffinity columns for sample clean-up and HPLC with fluorescence detection without any derivatisation step. The method showed good inter-day accuracy (bias values in the range 4.5-10.7%) and precision (5-16% RSD) when applied to the determination of levels of aflatoxins ranging from 7 to 20 ppb in the plant material. The detection limits for samples of the plant material spiked with aflatoxins were 3.5 ng/g for B1 and G1 and 0.1 ng/g for B2 and G2. The method was successfully applied to commercial samples of Maytenus ilicifolia for the screening of aflatoxin contaminants.
Braga
Sayonara Maria Lia Fook Meira
SM
UDEM/LTF, Universidade Federal da Paraíba, Caixa Postal 5044, CEP: 58051-970, João Pessoa, PB, Brazil.
de Medeiros
Francinalva Dantas
FD
de Oliveira
Eduardo Jesus
EJ
Macedo
Rui Oliveira
RO
D000348:Aflatoxins / Q000032:analysis*; D001938:Brazil; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029755:Maytenus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D000348:Aflatoxins; D010936:Plant Extracts