title: Porcine-derived xenogeneic bone/poly(glycolide-co-lactide-co-caprolactone) composite and its affinity with rat OCT-1 osteoblast-like cells.
abstract: Porcine-derived xenogeneic bone (PDXB) was derived from cancellous bone of adult porcine. Its morphology and structure were characterized by SEM, FTIR and XRD. A series of composite films consisting of PDXB and poly(glycolide-co-lactide-co-caprolactone) (PGLC) polymer were prepared. Because of the introduction of PGLC polymer, the PDXB/PGLC composites especially PDXB/PGLC(30/70) and PDXB/PGLC(50/50) showed good processability and mechanical properties. In addition, the hydrophilicity of the composites was enhanced as well since the PDXB component was hydrophilic. Osteoblast-like cells (OCT-1) were used as an in-vitro model to assess the affinity of the PDXB/PGLC composites. It was found that compared with the pure PGLC film, PDXB/PGLC(30/70) and PDXB/PGLC(50/50) composite films promoted cell attachment, proliferation and ALP (alkaline phosphatase) activity obviously. In addition, the cells preferred growing on the areas of exposed PDXB. It was considered that the hydrophilicity, osteoconductivity and appropriate surface roughness (Sa=3.30, 4.00 microm) induced by PDXB facilitate cell growth. However, the introduction of too much PDXB, such as PDXB/PGLC(70/30) film, would obtain an adverse effect on the cell growth since the value of Sa was up to 7.33 microm. It indicated that only the composites with appropriate surface topography could favor cell growth. Surface topography probably has a more important effect on cell growth process than surface chemistry.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Qu
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Wan
authors.forename: Hanwei
authors.initials: H
authors.lastname: Zhang
authors.forename: Wenjin
authors.initials: W
authors.lastname: Cui
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Bei
authors.forename: Shenguo
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry; D001842:Bone and Bones / Q000648:ultrastructure; D002460:Cell Line; D048430:Cell Shape; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties; D013552:Swine; D013718:Tensile Strength; D023822:Tissue Engineering; D014183:Transplantation, Heterologous; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D011091:Polyesters; D014867:Water; D011100:Polyglycolic Acid