[Expression of osteoclast differentiation factor and intercellular adhesion molecule-1 of bone marrow mesenchymal stem cells enhanced with osteogenic differentiation].
OBJECTIVE
To investgate whether the expression of osteoclast differentiation factor (ODF), intercellular adhesion molecule-1 (ICAM-1) depended on the stage of osteoblastic differentiation from rat bone marrow mesenchymal stem cells (BMSC).


METHODS
BMSC (4 passage) were selected for osteogenic differentiation by treated with osteogenic supplements (OS). Cells were harvested by day 0, 3, 6, 9, 12, 18 respectively after osteogenic inducement. In each experiment, control and OS-treated cells were processed in parallel. ODF and ICAM-1 mRNA were analyzed by semiquantitave RT-PCR assay.


RESULTS
Expression of ODF was enhanced with osteogenic differentiation guadully. whereas, expression of ICAM-1 was activated at OS-treated day 6, then keeping at a stable level.


CONCLUSIONS
This study indicated that BMSC undergoing osteogenic inducement was an ideal model for studying the differentiation and maturation of osteoblasts. During the early stage of differentiation along osteoblasts from stem cells to osteocytes, BMSC or osteoprogenitor react somewhat differently from osteoblasts, suggesting the ability of osteoblasts to regulating differentiation and maturation of osteoclasts have been improved with osteogenic culture.
Wang
Jun
J
Key Laboratory of Oral Biomedical Engineering, Ministry of Education, Sichuan University, Chengdu 610041, China.
Zhao
Zhi-He
ZH
Luo
Song-Jia
SJ
Fan
Yu-Bo
YB
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D018799:Intercellular Adhesion Molecule-1; D059630:Mesenchymal Stem Cells; D010006:Osteoblasts; D010010:Osteoclasts; D010012:Osteogenesis; D053245:RANK Ligand; D051381:Rats; D013234:Stem Cells
D053245:RANK Ligand; D018799:Intercellular Adhesion Molecule-1