title: Posttransplant hematopoiesis in patients undergoing sibling allogeneic stem cell transplantation reflects that of their respective donors although with a lower functional capability.
abstract: We tested the principle of whether patient long-term hematopoiesis following allogeneic stem cell transplantation (allo-SCT) reflects the characteristics of the hematopoiesis of their respective donor. For this purpose, we analyzed bone marrow (BM) hematopoiesis using long-term cultures (LTC), delta assays, and clonogeneic assays as well as CD34+ cells and their subsets by flow cytometry in a series of 37 patients undergoing allo-SCT, and we compared it to that of their respective human leukocyte antigen-matched sibling donors in a paired study performed more than 1 year after the transplant procedure. Interestingly, the main factor that influenced post-allo-SCT BM hematopoiesis in the long term was donor hematopoiesis. Nevertheless, compared to their respective donors, patients exhibited a significantly lower number of colony-forming units granulomonocytic, burst-forming units erythroid, and immature progenitors (CD34++/CD38dim/CD90+/CD133+ cells, LTC-initiating cells, and colonies generated in the delta assay). Moreover, BM stromal function was diminished in patients undergoing allo-SCT compared to their donors. In addition, the presence of chronic graft-versus-host disease under immunosuppressive treatment also conditioned an impaired hematopoietic function. In summary, our study shows that BM hematopoiesis evaluated more than 1 year after an allo-SCT mainly reproduces that of their respective donors, although with a significantly decreased in vitro activity.
authors.affiliation: Servicio de Hematologia, Hospital Universitario de Salamanca, Universidad de Salamanca, Spain.
authors.forename: Fermin M
authors.initials: FM
authors.lastname: Sanchez-Guijo
authors.forename: Luis-Ignacio
authors.initials: LI
authors.lastname: Sanchez-Abarca
authors.forename: Eva
authors.initials: E
authors.lastname: Villaron
authors.forename: Natalia
authors.initials: N
authors.lastname: Lopez-Holgado
authors.forename: Mercedes
authors.initials: M
authors.lastname: Alberca
authors.forename: Lourdes
authors.initials: L
authors.lastname: Vazquez
authors.forename: Jose A
authors.initials: JA
authors.lastname: Perez-Simon
authors.forename: Jesus
authors.initials: J
authors.lastname: Lopez-Fidalgo
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
authors.forename: Maria-Dolores
authors.initials: MD
authors.lastname: Caballero
authors.forename: Maria-Consuelo
authors.initials: MC
authors.lastname: Del Cañizo
authors.forename: Jesus F
authors.initials: JF
authors.lastname: San Miguel
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D015672:Erythroid Precursor Cells / Q000166:cytology; D005260:Female; D006086:Graft vs Host Disease / Q000503:physiopathology; D042381:Granulocyte Precursor Cells / Q000166:cytology; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D017154:Stromal Cells / Q000166:cytology; D018183:Transplantation Chimera; D014184:Transplantation, Homologous
chemical_list: D015703:Antigens, CD