Adrenomedullin modulates COX-2 and HGF expression in reserpine-injuried gastric mucosa in the rat.
Here we show the increased hepatocyte growth factor (HGF) and cyclooxygenase-2 (COX-2) expression in gastric mucosa of rats which have developed a reserpine-induced ulcer. Such an increase of HGF and COX-2 expression was blunted in rats pretreated with adrenomedullin. Pretreatment with adrenomedullin and the adrenomedullin22-52 fragment did not result in changes of HGF and COX-2 expression, compared to the reserpine and adrenomedullin treated group. Pretreatment with adrenomedullin and the calcitonin gene-related peptide8-37 fragment (CGRP8-37) increased HGF and COX-2 expression, compared to the reserpine and adrenomedullin treated group. Our results suggest that the inhibitory effect of adrenomedullin on the expression of HGF and COX-2 is mediated by CGRP receptors.
Cantarella
Giuseppina
G
Department of Experimental and Clinical Pharmacology, School of Medicine, University of Catania, Italy.
Martinez
Giuseppa
G
Cutuli
Vincenza Maria
VM
Loreto
Carla
C
D'Alcamo
Maria
M
Prato
Agata
A
Amico-Roxas
Matilde
M
Bernardini
Renato
R
Clementi
Giuseppe
G
D053607:Adrenomedullin; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D051546:Cyclooxygenase 2; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012110:Reserpine / Q000633:toxicity*
D010446:Peptide Fragments; D010455:Peptides; C058636:calcitonin gene-related peptide (8-37); D053607:Adrenomedullin; D017228:Hepatocyte Growth Factor; D012110:Reserpine; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D015740:Calcitonin Gene-Related Peptide