title: Production of inbred and hybrid transgenic mice carrying large (> 200 kb) foreign DNA fragments by intracytoplasmic sperm injection.
abstract: We have developed a mouse transgenesis technique that facilitates the insertion of large (approximately 200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.
authors.affiliation: Department of Anatomy and Reproductive Biology, The Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii, USA.
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Osada
authors.forename: Atsushi
authors.initials: A
authors.lastname: Toyoda
authors.forename: Stefan
authors.initials: S
authors.lastname: Moisyadi
authors.forename: Hidenori
authors.initials: H
authors.lastname: Akutsu
authors.forename: Masahira
authors.initials: M
authors.lastname: Hattori
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sakaki
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Yanagimachi
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D017931:DNA Primers; D004229:Dithiothreitol; D004314:Down Syndrome / Q000235:genetics*; D004624:Embryo Transfer; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*
chemical_list: D017931:DNA Primers; D004229:Dithiothreitol