Immunoassay signal amplification on glass slides based on electroless deposition.
Saturated coverage of antibodies was first constructed on glass slides coated with polyelectrolyte multilayer; then sandwich immunoreactions were carried out. Following immunoreactions, the glass slides were immersed in a freshly prepared solution containing 0.01 wt% HAuCl4 and 0.4 mM NH2OH.HCl. The growth of immunogold nanoparticles on glass slides was monitored with UV-vis spectrometer in real time. The data indicated that the immunoassay signal amplification really depends on the time of electroless deposition process and the concentration of antigen.
Su
Yan-Lei
YL
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China. suyanlei@tju.edu.cn
D004305:Dose-Response Relationship, Drug; D004573:Electrolytes; D005898:Glass; D007118:Immunoassay / Q000379:methods*; D007150:Immunohistochemistry; D007202:Indicators and Reagents; D049329:Nanostructures / Q000737:chemistry; D013053:Spectrophotometry; D013997:Time Factors; D014466:Ultraviolet Rays; D014961:X-Ray Diffraction
D004573:Electrolytes; D007202:Indicators and Reagents