title: Human papillomavirus DNA detection in women with primary abnormal cytology of the cervix: prevalence and distribution of HPV genotypes.
abstract: OBJECTIVE
  In this study, we focus on the prevalence and occurrence of different anogenital human papillomavirus (HPV) genotypes in a first abnormal cervical screening test, and correlate HPV genotyping with the cytological diagnosis on thin-layer liquid-based preparations in routine gynaecological screening.
  
  
  METHODS
  Out of 780 abnormal smears, 513 tested positive for HPV. All 25 different HPV types were identified by Line Probe Assay.
  
  
  RESULTS
  The prevalence of high-risk HPV types increased from 72% in atypical squamous cell of undetermined significance to 94.5% in high-grade intra-epithelial lesion (HSIL). Co-infection with multiple HPV types was predominantly found in HSIL (35.8%). In the HSIL group the most common HPV types were 16, 52, 51 and 31; type 18 was rarely present.
  
  
  CONCLUSIONS
  The role of types 31, 51 and 52 should be considered in future studies on vaccine development.
authors.affiliation: Department of Pathology, Ghent University Hospital, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Beerens
authors.forename: L
authors.initials: L
authors.lastname: Van Renterghem
authors.forename: M
authors.initials: M
authors.lastname: Praet
authors.forename: Y
authors.initials: Y
authors.lastname: Sturtewagen
authors.forename: S
authors.initials: S
authors.lastname: Weyers
authors.forename: M
authors.initials: M
authors.lastname: Temmerman
authors.forename: H
authors.initials: H
authors.lastname: Depypere
authors.forename: P
authors.initials: P
authors.lastname: Claeys
authors.forename: C A
authors.initials: CA
authors.lastname: Cuvelier
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D001530:Belgium / Q000453:epidemiology; D002584:Cervix Uteri / Q000473:pathology* / Q000821:virology*; D015897:Comorbidity; D003581:Cytodiagnosis; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D006801:Humans; D008403:Mass Screening / Q000379:methods; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology
chemical_list: D004279:DNA, Viral