title: Variant histone H3.3 is deposited at sites of nucleosomal displacement throughout transcribed genes while active histone modifications show a promoter-proximal bias.
abstract: Deposition of variant histones provides a mechanism to reset and to potentially specify chromatin states. We determined the distribution of H3 and its variant H3.3 relative to chromatin structure and elongating polymerase. H3.3 is enriched throughout active genes similar to polymerase, yet its distribution is very distinct from that of several euchromatic histone modifications, which are highly biased toward the 5' part of active genes. Upon gene induction we observe displacement of both H3 and H3.3 followed by selective deposition of H3.3. These results support a model in which H3.3 deposition compensates for transcription-coupled nucleosomal displacement yet does not predetermine tail modifications.
authors.affiliation: Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland.
authors.forename: Christiane
authors.initials: C
authors.lastname: Wirbelauer
authors.forename: Oliver
authors.initials: O
authors.lastname: Bell
authors.forename: Dirk
authors.initials: D
authors.lastname: Schübeler
mesh_terms: D000818:Animals; D004330:Drosophila; D005455:Fluorescent Antibody Technique; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D009707:Nucleosomes / Q000378:metabolism*; D011401:Promoter Regions, Genetic
chemical_list: D006657:Histones; D009707:Nucleosomes