title: Modelling and optimisation of enzymatic separating micro-reactor.
abstract: A mathematical model of an enzymatic separating microreactor with the electro-osmotic control of reaction component transport rates is analysed. The micro-reactor is considered in a form of a thin channel filled with a gel containing an immobilised enzyme and an adsorbent where the enzyme reaction, the molecular diffusion, the electro-osmotic flux and the adsorption take place. The substrate inhibited enzyme reaction splitting a non-ionic substrate to two non-ionic products is considered. The reactor operates in a periodic regime, when the channel entry is exposed to the periodic substrate concentration pulses. A chromatographic separation of reaction components, therefore, proceeds in the channel. Effects of principal operational parameters of the reactor system-the reaction channel length, the electric current density, the substrate inlet concentration, the rate of adsorption, and the enzyme activity--on resolution of the products at reactor outlet are analysed. The existence of optimum parameter values (maximising the resolution of reaction products) is shown and a multiparametric optimisation of the reactor performance is accomplished.
authors.affiliation: Department of Chemical Engineering & Center for Nonlinear Dynamics of Chemical and Biological Systems, Institute of Chemical Technology, Prague, 166 28, Prague 6, Czech Republic.
authors.forename: F
authors.initials: F
authors.lastname: Tmej
authors.forename: Z
authors.initials: Z
authors.lastname: Limbergová
authors.forename: P
authors.initials: P
authors.lastname: Hasal
mesh_terms: D019149:Bioreactors; D004800:Enzymes, Immobilized / Q000378:metabolism*; D008954:Models, Biological
chemical_list: D004800:Enzymes, Immobilized