[Research on DNA polymorphism in mixed biological traces].
The authors present the results of VNTR-PCR DNA polymorphism of three STR loci. Gene profiles were determined in experimental spots of blood from 2 or 3 unrelated individuals mixed in the ratios 1:1-1:100. Genotypes were successfully determined in mixed blood spot samples of ratios 1:1-1:60. A small amount of DNA in all those samples manifested a light electrophoretic staining. The electrophoretic band disappearance in the mixed blood spot samples of high dilution (<1:70) did not allow for determination of their genotypes. In the paper the results obtained while identifying biological traces from sexual offences in which mixed DNA occurred have been also presented.
Droździok
Kornelia
K
Slaskiej Akademii Medycznej w Katowicach.
Kabiesz
Jadwiga
J
D001801:Blood Stains; D004247:DNA / Q000097:blood*; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D005838:Genotype; D006801:Humans; D008297:Male; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012742:Sex Offenses; D013050:Spectrometry, Fluorescence; D020080:Tandem Repeat Sequences / Q000235:genetics*
D004247:DNA