title: The late blight resistance locus Rpi-bib3 from Solanum bulbocastanum belongs to a major late blight R gene cluster on chromosome 4 of potato.
abstract: Late blight, caused by Phytophthora infestans, is one of the most devastating diseases in cultivated potato. Breeding of new potato cultivars with high levels of resistance to P. infestans is considered the most durable strategy for future potato cultivation. In this study, we report the identification of a new late-blight resistance (R) locus from the wild potato species Solanum bulbocastanum. Using several different approaches, a high-resolution genetic map of the new locus was generated, delimiting Rpi-blb3 to a 0.93 cM interval on chromosome 4. One amplification fragment length polymorphism marker was identified that cosegregated in 1,396 progeny plants of an intraspecific mapping population with Rpi-blb3. For comparative genomics purposes, markers linked to Rpi-blb3 were tested in mapping populations used to map the three other late-blight R loci Rpi-abpt, R2, and R2-like also to chromosome 4. Marker order and allelic conservation suggest that Rpi-blb3, Rpi-abpt, R2, and R2-like reside in the same R gene cluster on chromosome 4 and likely belong to the same gene family. Our findings provide novel insights in the evolution of R gene clusters conferring late-blight resistance in Solanum spp.
authors.affiliation: Graduate School Experimental Plant Sciences, Laboratory of Plant Breeding, Department of Plant Sciences, Wageningen University, Binnenhaven 5, 6709 PD, Wageningen, The Netherlands.
authors.forename: Tae-Ho
authors.initials: TH
authors.lastname: Park
authors.forename: Jack
authors.initials: J
authors.lastname: Gros
authors.forename: Anne
authors.initials: A
authors.lastname: Sikkema
authors.forename: Vivianne G A A
authors.initials: VG
authors.lastname: Vleeshouwers
authors.forename: Marielle
authors.initials: M
authors.lastname: Muskens
authors.forename: Sjefke
authors.initials: S
authors.lastname: Allefs
authors.forename: Evert
authors.initials: E
authors.lastname: Jacobsen
authors.forename: Richard G F
authors.initials: RG
authors.lastname: Visser
authors.forename: Edwin A G
authors.initials: EA
authors.lastname: van der Vossen
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D023281:Genomics; D005810:Multigene Family; D010838:Phytophthora / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D011110:Polymorphism, Genetic; D032322:Solanum / Q000235:genetics* / Q000382:microbiology*; D011198:Solanum tuberosum / Q000235:genetics* / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D018744:DNA, Plant