Cell-based treatment of osteochondral defects in the rabbit knee with natural and synthetic matrices: cellular seeding determines the outcome.
BACKGROUND
Matrix-associated transplantation of cartilage constructs is an appealing method in cartilage repair. Three different matrices seeded with allogenic chondrocytes were compared in an osteochondral defect model in the rabbit. An investigation was conducted to identify the best matrix for cell-based treatment of osteochondral defects in the rabbit knee joint.


METHODS
Osteochondral defects (diameter 3 mm) were created in the trochlea and the femoral condyles of 33 New Zealand White rabbits, which were then treated with bioartificial cartilage constructs. The cartilage constructs were created in vitro using three different resorbable carrier materials (two fleece matrices: one of PLLA, and one composite of polydioxanon/ polyglactin, as well as one consisting of lyophilized dura) cultured with isolated allogenic chondrocytes. The defects were evaluated macroscopically, by histological and immunhistological techniques, and by scanning electron microscopy after 6 weeks, 6 months, and 12 months. The chondrocyte-seeded constructs were compared to defects treated with carrier material alone as well as to untreated control defects.


RESULTS
There was a significant improvement in defect repair quality in the transport materials, which were cultured with chondrocytes prior to implantation (P < 0.0005). No significant differences were observed between the three carrier matrices, and no significant differences were seen between the unseeded matrices and the untreated control defects.


CONCLUSIONS
There is no difference in the outcome between the three tested matrices in the treatment of osteochondral defects in the rabbit knee. The results of this in vitro experiment are promising and with refinement may lead to useful clinical therapies.
Rudert
M
M
Orthopaedic Department, University of Tübingen, Hoppe-Seyler-Str 3, 72076 Tübingen, Germany. maximilian.rudert@med.uni-tuebingen.de
Wilms
U
U
Hoberg
M
M
Wirth
C J
CJ
D000818:Animals; D002358:Cartilage, Articular / Q000293:injuries*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000637:transplantation*; D003094:Collagen / Q000378:metabolism; D005260:Female; D006614:Hindlimb; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods
D003094:Collagen