On-line coupling of gel electrophoresis and inductively coupled plasma-sector field-mass spectrometry for the determination of dsDNA fragments.
The on-line coupling of gel electrophoresis (GE) and inductively coupled plasma-mass spectrometry (ICPMS) is described for the first time. The new method combines the separation power of GE for large biomolecules and the high sensitivity and elemental selectivity of ICPMS. This coupling has been achieved by means of gels housed in glass tubes (length 2.5-20 cm; i.d. 0.5-5 mm). The gel is fixed by glass frits permeable for the analytes. After the electrophoretic separation at voltages up to 500 V, the analytes are transferred to the nebulizer of the ICPMS by an eluent stream, which is separated from the electrode chamber by a membrane. This filter blocks molecules with molecular masses larger than 500 Da. Using double-stranded DNA fragments, commercially available standard solutions were analyzed with on-line GE-ICPMS for the first time by monitoring (31)P(+) with a double-focusing mass spectrometer at a mass resolution of 4000.
Brüchert
Wolfram
W
Institute of Inorganic Chemistry and Analytical Chemistry, University of Mainz, Duesbergweg 10-14, D-55099 Mainz, Germany.
Bettmer
Jörg
J
D002138:Calibration; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*
D004247:DNA