Combined deficiency of IL-1beta18, but not IL-1alphabeta, reduces susceptibility to hypoxia-ischemia in the immature brain.
Interleukin (IL)-1 and IL-18 belong to the IL-1 family. IL-18 deficiency has been shown to confer moderate protection after hypoxia-ischemia (HI) in the immature brain, while the contribution of the two isoforms of IL-1 (IL-1alpha and IL-1beta) in neonatal HI brain injury has not been investigated previously. The aim of this study was to examine the contribution of the different members of the IL-1 family to neonatal HI damage. Unilateral HI was induced at postnatal day 9 in IL-1beta, IL-1beta18, and IL-1alphabeta knockout and wild-type mice and brain injury was evaluated 1 week later. IL-1beta18-deficient mice showed 17% reduction in brain injury, while no significant reduction in injury was detected between any of the other groups. These results indicate that IL-18, but not IL-1beta, or the combination of IL-1alpha and IL-1beta, is a contributor to HI injury in the immature brain.
Hedtjärn
Maj
M
Department of Physiology and Pharmacology, Perinatal Center, Goteborg University, Goteborg, Sweden. maj.hedtjarn@fysiologi.gu.se
Mallard
Carina
C
Iwakura
Yoichiro
Y
Hagberg
Henrik
H
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D017931:DNA Primers; D004198:Disease Susceptibility; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism* / Q000473:pathology; D007375:Interleukin-1 / Q000378:metabolism*; D020382:Interleukin-18 / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D007375:Interleukin-1; D020382:Interleukin-18