Determination of diarylheptanoids from Alpinia officinarum (Lesser Galangal) by HPLC with photodiode array and electrochemical detection.
Normal-phase column chromatography followed by semi-preparative reversed-phase HPLC has been used to isolate, from the rhizomes of Alpinia officinarum, five diarylheptanoids identified as 5-hydroxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone, 5-methoxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone, 7-(4"-hydroxyphenyl)-1-phenylhept-4-en-3-one, 7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-hept-4-en-3-one, 1,7-diphenylhept-4-en-3-one. The levels of these five diarylheptanoids in root material were determined quantitatively by HPLC with UV detection and the assay methods so developed were simple, rapid and accurate. Four of the diarylheptanoids could also be detected by HPLC with electrochemical detection (ECD) in the oxidative mode, and ECD was found to have a higher sensitivity than photodiode array detection.
Liu
Zhihua
Z
Food Science Department and Center for Advanced Food Technology, Cook College, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA.
Sang
Shengmin
S
Hartman
Thomas G
TG
Ho
Chi-Tang
CT
Rosen
Robert T
RT
D032429:Alpinia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D006536:Heptanes / Q000302:isolation & purification*; D007659:Ketones / Q000302:isolation & purification*; D013058:Mass Spectrometry; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D027343:Rhizome / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D004365:Drugs, Chinese Herbal; D006536:Heptanes; D007659:Ketones