title: Genetic control of experimental spondylarthropathy.
abstract: OBJECTIVE
  To characterize experimentally induced spondylarthropathy (SpA) in arthritis-susceptible inbred mice and in their F(1) and F(2) hybrid generations of susceptible and resistant mouse strains.
  
  
  METHODS
  SpA was induced in susceptible BALB/c and C3H/HeJCr (C3H) strains of mice, and in their F(1) and F(2) generations derived from intercrosses with arthritis- and/or spondylitis-resistant DBA/2 and DBA/1 parent strains, by systemic immunization with cartilage proteoglycan (PG) aggrecan. The incidence and severity of PG-induced spondylitis (PGIS) were scored histologically, and these scores for spine involvement were correlated with serum antibody and cytokine levels and with in vitro T cell responses to cartilage PG.
  
  
  RESULTS
  PGIS was induced by systemic immunization with cartilage PG in adjuvant, and approximately 60-70% of susceptible mouse strains and their F(2) hybrids developed spondylitis either with or without arthritis. Adjuvants, particularly those activating the innate immune system and enforcing the Th1 dominance, had significant effects on the outcome and progression of SpA. The DBA/1 strain appeared to carry genes protecting this strain and its F(1) and F(2) hybrids from spondylitis, whereas the DBA/2 strain, although resistant to PGIS, harbored genes permitting PGIS in its hybrid generations. Arthritis- and/or spondylitis-susceptible BALB/c and C3H parent strains and their F(2) hybrids exhibited the highest incidence and severity of spondylitis.
  
  
  CONCLUSIONS
  PGIS, a murine model of autoimmune spondylitis, shows similarities to ankylosing spondylitis. Segregation of susceptibility to PG-induced arthritis (PGIA) from that to PGIS in different genetic crosses suggests that PGIA and PGIS are separate diseases. Therefore, this model allows for the elucidation of genetic components involved in the etiology of SpA, independent of those controlling the susceptibility to PGIA.
authors.affiliation: University of Debrecen, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szabó
authors.forename: Sándor
authors.initials: S
authors.lastname: Szántó
authors.forename: Anikó
authors.initials: A
authors.lastname: Végvári
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szekanecz
authors.forename: Katalin
authors.initials: K
authors.lastname: Mikecz
authors.forename: Tibor T
authors.initials: TT
authors.lastname: Glant
mesh_terms: D053674:Aggrecans; D000818:Animals; D001168:Arthritis / Q000000981:diagnostic imaging / Q000235:genetics / Q000276:immunology / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000276:immunology*; D005260:Female; D020022:Genetic Predisposition to Disease; D006824:Hybridization, Genetic; D015994:Incidence; D037181:Lectins, C-Type; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics; D011509:Proteoglycans / Q000276:immunology*; D011859:Radiography; D012720:Severity of Illness Index; D013045:Species Specificity; D025242:Spondylarthropathies / Q000000981:diagnostic imaging / Q000235:genetics* / Q000276:immunology* / Q000473:pathology
chemical_list: C508320:Acan protein, mouse; D053674:Aggrecans; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011509:Proteoglycans