title: Methods for the culture of C. elegans and S. cerevisiae in microgravity.
abstract: To support the study of the effects of microgravity on biological systems, our group is developing and testing methods that allow the cultivation of C. elegans and S. cerevisiae in microgravity. Our aim is to develop the experimental means by which investigators may conduct peer reviewed biological experiments with C. elegans or S. cerevisiae in microgravity. Our protocols are aimed at enabling investigators to grow these organisms for extended periods during which samples may be sub-cultured, collected, preserved, frozen, and/or returned to earth for analysis. Data presented include characterization of the growth phenotype of these organisms in liquid medium in OptiCells(TM) (Biocrystal, LTD).
authors.affiliation: Lockheed Martin Space Operations, NASA Ames Research Center, Moffett Field, CA 94035, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Fahlen
authors.forename: June
authors.initials: J
authors.lastname: Sunga
authors.forename: Jon
authors.initials: J
authors.lastname: Rask
authors.forename: Anna
authors.initials: A
authors.lastname: Herrera
authors.forename: Kitty
authors.initials: K
authors.lastname: Lam
authors.forename: Luke
authors.initials: L
authors.lastname: Sing
authors.forename: Kevin
authors.initials: K
authors.lastname: Sato
authors.forename: Ross A
authors.initials: RA
authors.lastname: Ramos
authors.forename: Melissa
authors.initials: M
authors.lastname: Kirven-Brooks
authors.forename: Debra
authors.initials: D
authors.lastname: Reiss-Bubenheim
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000254:growth & development*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D005069:Evaluation Studies as Topic; D008021:Life Support Systems; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D014893:Weightlessness
chemical_list: D003470:Culture Media