Bronchoalveolar lavage examined by solid phase microextraction, gas chromatography--mass spectrometry and selected ion flow tube mass spectrometry.
Samples (210 in total) of broncholaveolar lavages (BALs), obtained from patients hospitalized with pneumonia in various departments of two hospitals, were analysed using the method of solid phase microextraction-gas chromatography (SPME-GC) with FID detection. Up to 20% (9% unequivocally, 11% probably) of these samples was found to contain volatile fatty acids (VFAs) in the series from acetic acid to heptanoic acid. Importantly, the presence of these acids indicates the presence of fermenting anaerobic bacteria, which were not detected by the conventional microbiological examination. Other compounds, namely the heptanol and cyclohexanone, were also detected by this method in some samples. Cyclohexanone occurred almost exclusively in samples from patients receiving intensive care with mechanical ventilation, and is suspected to originate from plastic parts of ventilators. Selected representative samples were also analysed using further methods, namely gas chromatography-mass spectrometry (GC-MS) of native and silylated samples, and selected ion flow tube mass spectrometry (SIFT-MS). These methods confirmed the identities of above mentioned compounds, and detected numerous other compounds tentatively identified as various alcohols, aldehydes, ketones, esters and hydrogen cyanide, HCN. Most of these compounds occurred in small amounts and their origin and diagnostic significance remains uncertain, except, that is, for the HCN, which indicates the presence of Pseudomonas aeruginosa.
Julák
Jaroslav
J
Institute of Immunology and Microbiology, The First Faculty of Medicine, Charles University, Studnićkova 7, 128 08 Praha 2, Czech Republic. jaroslav.julak@lfl.cuni.cz
Stránská
Eva
E
Rosová
Vlasta
V
Geppert
Helmut
H
Spanel
Patrik
P
Smith
David
D
D001421:Bacteria, Anaerobic / Q000302:isolation & purification* / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000382:microbiology*; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D018410:Pneumonia, Bacterial / Q000378:metabolism / Q000382:microbiology*
D005232:Fatty Acids, Volatile