title: Comparison of silanization/hydrosilation and organosilanization modification procedures on etched capillaries for electrokinetic chromatography.
abstract: Etched capillaries for use in open tubular electrochromatography are modified by silanization/hydrosilation and organosilanization. The migration behavior of both types of capillaries is evaluated with small basic molecules, peptides and proteins. Comparisons of peak symmetry and efficiency are used to measure the effectiveness of the two methods for modifying the etched surface. From this information, the suitability of each method for use with etched capillaries can be determined.
authors.affiliation: Department of Chemistry, San Jose State University, One Washington Square, San Jose, CA 95192, USA.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Matyska
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Pesek
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D045304:Cytochromes c / Q000302:isolation & purification; D009113:Muramidase / Q000302:isolation & purification; D017646:Organosilicon Compounds / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D017646:Organosilicon Compounds; D045304:Cytochromes c; D009113:Muramidase