Comparison of measurements of canine plasma creatinine, glucose, proteins, urea, alanine aminotransferase, and alkaline phosphatase obtained with Spotchem SP 4430 and Vitros 250 analyzers.
The suitability of the Spotchem 4430 benchtop biochemistry analyzer for canine blood samples was tested for creatinine, glucose, proteins, urea, alkaline phosphatases and alanine aminotransferase. Results obtained from whole blood and corresponding heparin plasma were identical except for proteins which were higher in plasma (n=10). Between series imprecision (n=10) was <5% for substrates and <10% for enzymes. Comparison of results from 100 Li-heparin samples with those measured with a Vitros 250 analyzer showed good correlation (r>0.93). The slopes of the Passing-Bablock's regression ranged from 0.90 to 1.20 and intercepts were low. The mean biases were low, except for creatinine for which the results obtained by Spotchem (Jaffe reaction) were about 20 micromol/L higher than with the Vitros (enzymatic reaction). The results of this study show that the Spotchem analyzer is suitable for use in canine whole blood or plasma when small numbers of tests are to be performed and large analyzers are not available.
Trumel
C
C
Département des Sciences cliniques des animaux de sport et de loisirs, Ecole nationale vétérinaire, 23 Chemin des Capelles, 31076--Toulouse Cedex 3, France. c.trumel@envt.fr
Diquélou
A
A
Germain
C
C
Palanché
F
F
Braun
J P
JP
D000410:Alanine Transaminase / Q000097:blood*; D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D001331:Automation; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000662:veterinary*; D001786:Blood Glucose / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis*; D003404:Creatinine / Q000097:blood*; D004285:Dogs; D014508:Urea / Q000097:blood*
D001786:Blood Glucose; D001798:Blood Proteins; D014508:Urea; D003404:Creatinine; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase