Preparation and evaluation of poly(polyethylene glycol methyl ether acrylate-co-polyethylene glycol diacrylate) monolith for protein analysis.
A poly(polyethylene glycol methyl ether acrylate-co-polyethylene glycol diacrylate) monolith was prepared by UV-initiated polymerization. Methanol and ethyl ether were selected as porogens from a variety of organic solvents to achieve the desirable characteristics of the monolith. The preparation of the monolith could be achieved within 10 min. The monolith was macroscopically homogeneous, had low flow resistance, and did not swell or shrink significantly in tetrahydrofuran. Inverse size-exclusion data indicate that the monolith had a total porosity of 75.4% and an internal porosity of 9.1%. The monolith could be used for size-exclusion separation of peptides, although it could not separate proteins with molecular masses between 10 and 100 K due to its unique pore size distribution. It was found to resist adsorption of proteins in capillary liquid chromatography when using 100 mM phosphate buffer (pH 7.0) containing 0.5 M NaCl. Complete recovery of both acidic and basic proteins was achieved. The monolith can be used for applications in which inert materials are required for protein analysis.
Gu
Binghe
B
Department of Chemistry and Biochemistry, Brigham Young University, P O. Box 25700, Provo, UT 84602-5700, USA.
Armenta
Jenny M
JM
Lee
Milton L
ML
D000179:Acrylates / Q000138:chemical synthesis / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D010455:Peptides / Q000032:analysis; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*
D000179:Acrylates; D010455:Peptides; D011108:Polymers; D011109:Polymethacrylic Acids; D011506:Proteins; C437167:poly(ethylene glycol)diacrylate; C503797:poly(polyethylene glycol methyl ether acrylate-co-polyethylene glycol diacrylate); D011092:Polyethylene Glycols; C035956:methyl acrylate