Streptococcus pyogenes collagen type I-binding Cpa surface protein. Expression profile, binding characteristics, biological functions, and potential clinical impact.
The Streptococcus pyogenes collagen type I-binding protein Cpa (collagen-binding protein of group A streptococci) expressed by 28 serotypes of group A streptococci has been extensively characterized at the gene and protein levels. Evidence for three distinct families of cpa genes was found, all of which shared a common sequence encoding a 60-amino acid domain that accounted for selective binding to type I collagen. Surface plasmon resonance-based affinity measurements and functional studies indicated that the expression of Cpa was consistent with an attachment role for bacteria to tissue containing collagen type I. A cpa mutant displayed a significantly decreased internalization rate when incubated with HEp-2 cells but had no effect on the host cell viability. By utilizing serum from patients with a positive titer for streptolysin/DNase antibody, an increased anti-Cpa antibody titer was noted for patients with a clinical history of arthritis or osteomyelitis. Taken together, these results suggest Cpa may be a relevant matrix adhesin contributing to the pathogenesis of S. pyogenes infection of bones and joints.
Kreikemeyer
Bernd
B
Department of Medical Microbiology and Hospital Hygiene, Hospital of Rostock University, Schillingallee 70, D-18057 Rostock, Germany.
Nakata
Masanobu
M
Oehmcke
Sonja
S
Gschwendtner
Caroline
C
Normann
Jana
J
Podbielski
Andreas
A
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001665:Binding Sites; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D017930:Genes, Reporter; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms / Q000378:metabolism; D008156:Luciferases / Q000032:analysis / Q000378:metabolism; D008970:Molecular Weight; D016254:Mutagenesis, Insertional; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012703:Serotyping; D013290:Streptococcal Infections / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D020349:Surface Plasmon Resonance
D001426:Bacterial Proteins; D002352:Carrier Proteins; D024042:Collagen Type I; D011993:Recombinant Fusion Proteins; D008156:Luciferases