Effects of advanced glycation end products on ezrin-dependent functions in LLC-PK1 proximal tubule cells.
We have recently shown that advanced glycation products (AGEs) bind to the ERM (ezrin, radixin, moesin) family of proteins. ERM proteins act as cross-linkers between cell membrane proteins and the actin cytoskeleton. They are also involved in signal transduction pathways. They therefore have a critical role in normal cell processes, including modulation of cell shape, adhesion, and motility. We postulate that AGEs may contribute to diabetic complications by disrupting ERM function. In support of this hypothesis, AGEs inhibit ezrin-dependent tubulogenesis of proximal tubule cells. Phosphorylation is an important activating mechanism for ERM proteins, and AGEs inhibit ezrin phosphorylation mediated by the epidermal growth factor receptor.
Bach
Leon A
LA
University of Melbourne, Department of Medicine, Austin Hospital, Heidelberg, Vic 3084 Australia. leon.bach@med.monash.edu.au
Gallicchio
Marisa A
MA
McRobert
E Anne
EA
Tikoo
Anjali
A
Cooper
Mark E
ME
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D003598:Cytoskeletal Proteins; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000502:physiology*; D008565:Membrane Proteins / Q000502:physiology; D010750:Phosphoproteins / Q000502:physiology*; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000502:physiology; D000099149:Ezrin
D003598:Cytoskeletal Proteins; D017127:Glycation End Products, Advanced; D008565:Membrane Proteins; D010750:Phosphoproteins; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D000099149:Ezrin