Tagatose production by immobilized recombinant Escherichia coli cells containing Geobacillus stearothermophilus l-arabinose isomerase mutant in a packed-bed bioreactor.
Using immobilized recombinant Escherichia coli cells containing Geobacillus stearothermophilus l-arabinose isomerase mutant (Gali 152), we found that the galactose isomerization reaction was maximal at 70 degrees C and pH 7.0. Manganese ion enhanced galactose isomerization to tagatose. The immobilized cells were most stable at 60 degrees C and pH 7.0. The cell and substrate concentrations and dilution rate were optimal at 34 g/L, 300 g/L, and 0.05 h(-1), respectively. Under the optimum conditions, the immobilized cell reactor with Mn2+ produced an average of 59 g/L tagatose with a productivity of 2.9 g/L.h and a conversion yield of 19.5% for the first 20 days. The operational stability of immobilized cells with Mn2+ was demonstrated, and their half-life for tagatose production was 34 days. Tagatose production was compared for free and immobilized enzymes and free and immobilized cells using the same mass of cells. Immobilized cells produced the highest tagatose concentration, indicating that cell immobilization was more efficient for tagatose production than enzyme immobilization.
Jung
Eun-Sook
ES
Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea.
Kim
Hye-Jung
HJ
Oh
Deok-Kun
DK
D019747:Aldose-Ketose Isomerases / Q000235:genetics* / Q000378:metabolism; D019149:Bioreactors; D018914:Cells, Immobilized; D004867:Equipment Design; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D006601:Hexoses / Q000096:biosynthesis* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D008345:Manganese / Q000494:pharmacology; D008670:Metals / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature
D006601:Hexoses; D008670:Metals; D011994:Recombinant Proteins; D008345:Manganese; D019747:Aldose-Ketose Isomerases; C019714:D-arabinose isomerase; C030192:tagatose