In vitro micropropagation of Boswellia ovalifoliolata.
A protocol for micropropagation of Boswellia ovalifoliolata Bal & Henry (Burseraceae) was developed using cotyledonary nodal explant on Murashige and Skoog modified medium (MS). A comparative study of micropropagation with 6-benzyladenine, kinetin and thidiazuron along with 1-naphthalene acetic acid (0.054 microM) was conducted. The highest shoot multiplication (7.1 +/- 0.2 shoots per node) was achieved in 50 d on MS supplemented with thidiazuron (2.72 microM). Excised shoot cuttings of 3.0 cm were placed on the MS basal medium supplemented with indole-3-acetic acid and indole-3-butyric acid alone and in combinations for rooting. Activated charcoal (100 mg l(-1)) and polyvinylpyrrolidone (40 mg l(-1)) were added to the medium to prevent browning of cultures. The regenerated plantlets have been successfully acclimatized and transferred to soil.
Chandrasekhar
Thummala
T
Department of Botany, Sri Venkateswara Univerity, AP, India. chandrasekhart2k@yahoo.com
Hussain
T Mohammad
TM
Jayanand
Boddu
B
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000613:Aminobutyrates / Q000494:pharmacology; D029743:Boswellia / Q000502:physiology*; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D018525:Germination / Q000502:physiology*; D007701:Kinetin; D010671:Phenylurea Compounds / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D012098:Reproduction; D012639:Seeds / Q000502:physiology*; D013830:Thiadiazoles / Q000494:pharmacology
D000613:Aminobutyrates; D010671:Phenylurea Compounds; D010937:Plant Growth Regulators; D013830:Thiadiazoles; C016785:thidiazuron; C014328:2-amino-4-phenylbutyric acid; D000225:Adenine; D007701:Kinetin