Effect of acute alcohol intake on prostate tissue and serum PSA-like protein levels in rats.
OBJECTIVE
To investigate both the possible changes induced by acute alcohol intake on prostate tissue at the ultrastructural level and its effect on serum prostate-specific-antigen (PSA)-like protein levels in rats.


METHODS
44 male Wistar rats were included in the study in four separate groups; 7 treatment and 4 control animals were selected for each group. The treatment group received 40% ethanol (6 g/kg) while the control group was injected with the same dose of intraperitoneal isotonic saline. The first group was sacrificed after 3 h, the second group after 24 h, the third group after 72 h and the fourth group on day 7. Samples were examined by light and Jeol-100 electron microscope. Total serum PSA-like protein levels were determined by a Tosoh immunoenzymometric analyzer AIA 600.


RESULTS
In groups 1-3, electron microscopy showed dilatations in the endoplasmic reticulum cisternae of prostatic acinar cells, disarrangements in apparent Golgi complex and apertures belonging to the basal labyrinth. It was also observed that there was a mass of debris cells inside the acinar lumen, and the secretory epithelium was detached from the basal membrane in some places. No pathology was found in group 4 by light and electron microscopy. In group 1, significant levels of increased serum total PSA-like protein were found compared to controls and other treatment groups (p = 0.006).


CONCLUSIONS
The fundamental effect of acute alcohol intake on prostate tissue was observed in intermediate stages participating in the exocrine secretion process in the cellular organelles. At the same time, this influences the levels of serum PSA-like protein.
Gumus
Eyup
E
2nd Urology Clinic, Sisli Etfal Training and Research Hospital, Istanbul, Turkey. eyupg@superonline.com
Solakoglu
Seyhun
S
Mutus
Rifat
R
Altay
Bulent
B
Kiziler
Ali Riza
AR
Miroglu
Cengiz
C
D000208:Acute Disease; D000435:Alcoholic Intoxication / Q000097:blood / Q000473:pathology*; D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000097:blood; D002492:Central Nervous System Depressants / Q000008:administration & dosage / Q000633:toxicity*; D004195:Disease Models, Animal; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D007124:Immunoenzyme Techniques; D007274:Injections, Intraperitoneal; D008297:Male; D008854:Microscopy, Electron; D011467:Prostate / Q000187:drug effects* / Q000648:ultrastructure*; D017430:Prostate-Specific Antigen / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D002492:Central Nervous System Depressants; D000431:Ethanol; D017430:Prostate-Specific Antigen