title: Synergistic recognition of an epigenetic DNA element by Pleiohomeotic and a Polycomb core complex.
abstract: Polycomb response elements (PREs) are cis-acting DNA elements that mediate epigenetic gene silencing by Polycomb group (PcG) proteins. Here, we report that Pleiohomeotic (PHO) and a multiprotein Polycomb core complex (PCC) bind highly cooperatively to PREs. We identified a conserved sequence motif, named PCC-binding element (PBE), which is required for PcG silencing in vivo. PHO sites and PBEs function as an integrated DNA platform for the synergistic assembly of a repressive PHO/PCC complex. We termed this nucleoprotein complex silenceosome to reflect that the molecular principles underpinning its assemblage are surprisingly similar to those that make an enhanceosome.
authors.affiliation: Department of Biochemistry, Centre for Biomedical Genetics, Erasmus University Medical Center, 3000 DR Rotterdam, The Netherlands.
authors.forename: Adone
authors.initials: A
authors.lastname: Mohd-Sarip
authors.forename: Fabienne
authors.initials: F
authors.lastname: Cléard
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Mishra
authors.forename: François
authors.initials: F
authors.lastname: Karch
authors.forename: C Peter
authors.initials: CP
authors.lastname: Verrijzer
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D044127:Epigenesis, Genetic; D020868:Gene Silencing; D063150:Polycomb Repressive Complex 1; D063146:Polycomb-Group Proteins; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C066770:Pc protein, Drosophila; D063146:Polycomb-Group Proteins; D014157:Transcription Factors; C113295:pho protein, Drosophila; D004247:DNA; D063150:Polycomb Repressive Complex 1