title: Effects of age and caloric restriction on lipid peroxidation: measurement of oxidative stress by F2-isoprostane levels.
abstract: The free radical theory of aging proposes that the accumulation of oxidative damage is a key component of the aging process. The discovery of F2-isoprostanes (F2-isoPs) and their establishment as a sensitive and accurate biomarker of lipid peroxidation represents a major advance for measuring the oxidative stress status of an organism. We have shown that plasma free and total (free plus esterified) F2-isoPs increase with age (185% and 66%, respectively), and that these increases are reduced by life-extending caloric restriction (50% and 23%, respectively). In addition, we found that levels of esterified F2-isoPs increase 68% with age in liver, and 76% with age in kidney. Caloric restriction modulated the age-related increase, reducing the esterified F2-isoPs levels 27% in liver and 35% in kidney. These age-related increases in esterified F2-isoPs levels correlate well with DNA oxidation, as measured by 8-oxodeoxyguanosine production demonstrating that F2-isoPs are an excellent biomarker for age-related changes in oxidative damage to membranes.
authors.affiliation: Department of Physiology-MSC-7756, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA. wardw@uthscsa.edu
authors.forename: Walter F
authors.initials: WF
authors.lastname: Ward
authors.forename: Wenbo
authors.initials: W
authors.lastname: Qi
authors.forename: Holly
authors.initials: H
authors.lastname: Van Remmen
authors.forename: William E
authors.initials: WE
authors.lastname: Zackert
authors.forename: L Jackson
authors.initials: LJ
authors.lastname: Roberts
authors.forename: Arlan
authors.initials: A
authors.lastname: Richardson
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000367:Age Factors; D000375:Aging / Q000502:physiology*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D031204:Caloric Restriction; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D028441:F2-Isoprostanes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D015415:Biomarkers; D028441:F2-Isoprostanes; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine