[Expression and significance of PTEN and Survivin between the primary foci and the lymph node metastases foci of laryngeal squamous cell carcinoma].
OBJECTIVE
To investigate the molecular difference and significance between the primary foci and metastases foci of laryngeal squamous cell carcinoma (LSCC).


METHODS
Tissues of 18 cases of positive lymph node metastases (N+) LSCC were detected by using SP method. The LSCC primary foci and metastases foci and negative metastases lymph nodes were included, 22 cases of vocal cord polyp (VCP) were regarded as control. expression of PTEN and Survivin were evaluated by SP immunohistochemistry.


RESULTS
Expression of neither PTEN nor Survivin were positive in negative nodes. The positive rates of PTEN in LSCC, N+, VCP were 94.4% (17/18), 94.4% (17/18) and 95.5% (21/22) respectively, there were no significant difference among them (P>0.05). But the expression degree were more powerful in N+ than in the LSCC (P<0.05). There was no expression of Survivin in VCP. The positive rates of Survivin in LSCC and N+ were 55.5% (10/18), 50.0% (9/18), the difference were significant (P<0.01); but between LSCC and N+, neither the positive rate nor the expression degree had significant difference (P>0.05).


CONCLUSIONS
There were differences between the LSCC primary foci and the lymph node metastases foci in molecular level probably.
He
Long
L
Department of Otorhinolaryngology, the First Affiliated Hospital of Jinan University, Guangzhou, 510630, China. helong@126.com
Wang
Jiqun
J
Shan
Yanchun
Y
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000556:secondary; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D008875:Middle Aged; D009333:Neck; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis*; D000077022:Survivin
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D000077022:Survivin; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human