The zinc uptake regulator Zur is essential for the full virulence of Xanthomonas campestris pv. campestris.
Zur is a regulator of the high-affinity zinc uptake system in many bacteria. In Xanthomonas campestris pv. campestris 8004, a putative protein encoded by the open reading frame designated as XC1430 shows 42% amino acid similarity with the Zur of Escherichia coli. An XC1430-disrupted mutant 1430nk was constructed by homologous suicide plasmid integration. 1430nk failed to grow in rich medium supplemented with Zn2+ at a concentration of 400 microM and in nonrich medium supplemented with Zn2+ at a concentration of 110 microM, whereas the wild-type strain grew well in the same conditions. In rich medium with 400 microM Zn2+, 1430nk accumulated significantly more Zn2+ than the wild-type strain. 1430nk showed a reduction in virulence on the host plant Chinese radish (Raphanus sativus L. var. radiculus Pers.) and produced less extracellular polysaccharide (EPS) than did the wild-type strain in the absence of added zinc. These results revealed that XC1430 is a functional member of the Zur regulator family that controls zinc homeostasis, EPS production, and virulence in X. campestris pv. campestris.
Tang
Dong-Jie
DJ
The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China.
Li
Xiang-Jiang
XJ
He
Yong-Qiang
YQ
Feng
Jia-Xun
JX
Chen
Baoshan
B
Tang
Ji-Liang
JL
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010935:Plant Diseases / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D031224:Raphanus / Q000382:microbiology; D017386:Sequence Homology, Amino Acid; D014774:Virulence / Q000235:genetics; D016959:Xanthomonas campestris / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D015032:Zinc / Q000378:metabolism*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D011135:Polysaccharides, Bacterial; C114819:Zur protein, E coli; D015032:Zinc