title: [Morphological and functional characteristics of lymphokine-activated killer cells generated from the mononuclear cells of human peripheral blood].
abstract: The aim of this research was to study the morphological, functional and immunophenotypical characteristics of lymphokine-activated killer cells (LAKC) generated from the mononuclear cells (MNC) of healthy donors' peripheral blood at different time intervals after the cultivation with interleukin-2 (IL-2). LAKC had the appearance of large lymphoid cells of prolymphocyte and immunoblast type with highly pyroninophilic cytoplasm and electrone-microscopic features indicative of synthetic activity. LAKC were shown to intensely express activation antigens and adhesion molecules on their surface and to posess high cytotoxic potential in respect to tumor cells. Time-course of LAKC surface antigen expression corresponded to the changes of a proportion of activated cellular forms, generated from MNC of healthy donors' peripheral blood by incubation with IL-2. On the basis of these experimental findings, the usage of 3-5-day culture of LAKC could be recommended for the immunotherapy of malignant tumors.
authors.forename: O V
authors.initials: OV
authors.lastname: Lebedinskaia
authors.forename: E O
authors.initials: EO
authors.lastname: Khalturina
authors.forename: N K
authors.initials: NK
authors.lastname: Akhmatova
authors.forename: I Zh
authors.initials: IZh
authors.lastname: Shubina
authors.forename: O G
authors.initials: OG
authors.lastname: Leonova
authors.forename: V I
authors.initials: VI
authors.lastname: Popenko
authors.forename: S V
authors.initials: SV
authors.lastname: Melekhin
authors.forename: M V
authors.initials: MV
authors.lastname: Kiselevskiĭ
mesh_terms: D036541:ADP-ribosyl Cyclase / Q000276:immunology; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD / Q000276:immunology; D018968:CD58 Antigens / Q000276:immunology; D016923:Cell Death / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000378:metabolism; D020014:K562 Cells; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins; D009000:Monocytes / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D015375:Receptors, Interleukin-2 / Q000276:immunology
chemical_list: D015703:Antigens, CD; D018968:CD58 Antigens; D006684:HLA-DR Antigens; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D015375:Receptors, Interleukin-2; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1