title: Potential application of synchronous fluorescence spectroscopy to determine benzo[a]pyrene in soil extracts.
abstract: Benzo[a]pyrene (BaP) is a significant environmental pollutant and rapid, accurate methods to quantify this compound in soil for both research and environmental investigation purposes are required. In this work, solvent extracts from five contrasting soils spiked with four different polycyclic aromatic hydrocarbons (PAHs) were rapidly analysed by using a synchronous fluorescence spectroscopy (SFS) method. The SFS method was validated using HPLC with ultraviolet detection. A good correlation for the quantification of BaP in soil extracts by the two methods was observed. The detection limit of the SFS method was 1.6 x 10(-9) g/ml in CTAB micellar medium (7.8 mmol/l). The work demonstrates that SFS has potential as a sensitive, accurate, rapid, simple and economic methodology and an efficient alternative to HPLC for fast confirmation and quantification of BaP in complex soil extracts.
authors.affiliation: School of Biology, Institute for Research on the Environment and Sustainability, Devonshire Building, University of Newcastle upon Tyne, NE1 7RU, UK.
authors.forename: Guoxiong
authors.initials: G
authors.lastname: Hua
authors.forename: Ken
authors.initials: K
authors.lastname: Killham
authors.forename: Ian
authors.initials: I
authors.lastname: Singleton
mesh_terms: D001564:Benzo(a)pyrene / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D012989:Soil Pollutants / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D001564:Benzo(a)pyrene