Relationship among lipoperoxides, jasmonates and indole-3-acetic acid formation in potato tuber after wounding.
Plant responses to biotic and abiotic stress can be mediated by oxidised products and in this study we analysed the relation among some of them and the growth factor indole-3-acetic acid (IAA). The plant material used was potato tuber sliced below bud and incubated for different lengths of time before analysis. Wounding in potato tuber leads, in a very short time (0-30?min), to the generation of lipid hydroperoxides (LOOH) from polyunsaturated fatty acids (PUFA). These reactive species could cause a subsequent increase of 9 and 13-lipoxygenase (LOX, E.C.1.13.12.12.), analysed by RT-PCR and spectrophotometric assay, LOOH, Jasmonates and IAA all quantified by GC-MS analysis. The activation of 9 and 13-LOX, using different timing, leads to the formation of LOOH with a subsequent generation of jasmonates and IAA as highlighted by the addition on the potato tuber slices of salicylhydroxamic acid (SHAM), an inhibitor of LOX activity. A correlation between jasmonates and IAA resulted by testing their reciprocal influence during wounding in potato tuber. The relationship occurring among each hormone analysed during wounding underlines the fact that the jasmonates level can be regulated in situ and this can suggest a role for these compounds in potato tuber which has been underestimated up to now.
Reverberi
Massimo
M
Dipartimento Biologia Vegetale, Università La Sapienza, Largo Cristina di Svezia, 24, Rome, 00165, Italy. massimo.reverberi@uniroma1.it
Fanelli
Corrado
C
Zjalic
Slaven
S
Briganti
Stefania
S
Picardo
Mauro
M
Ricelli
Alessandra
A
Fabbri
Anna Adele
AA
D003517:Cyclopentanes / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007210:Indoleacetic Acids / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D008084:Lipoxygenase / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D054883:Oxylipins; D010935:Plant Diseases; D012333:RNA, Messenger / Q000235:genetics; D011198:Solanum tuberosum / Q000378:metabolism*; D013997:Time Factors
D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D007210:Indoleacetic Acids; D008054:Lipid Peroxides; D016859:Lipoxygenase Inhibitors; D054883:Oxylipins; D012333:RNA, Messenger; C011006:jasmonic acid; C030737:indoleacetic acid; D008084:Lipoxygenase