title: Development of two cytogenetically abnormal clones from multipotential hematopoietic stem cells in a patient with juvenile myelomonocytic leukemia.
abstract: We report a patient with juvenile myelomonocytic leukemia who had two cytogenetically independent clones at the time of diagnosis. Fluorescence in situ hybridization analyses showed that 42.5% of myeloperoxidase(+) cells and 27.3% of CD20(+) cells had three signals for chromosome 8, while 13.1% of myeloperoxidase(+) cells and 6.0% of CD20(+) cells had del (Y). However, a great majority of CD3(+) cells had no numerical aberration of the two chromosomes. The two karyotypically abnormal clones might have developed from multipotential hematopoietic stem cells with the potential to differentiate into myeloid and B-lymphoid lineages, but not T-lymphoid lineage.
authors.affiliation: Department of Pediatrics, Shinshu University School of Medicine, 3-1-1, Asahi, Matsumoto, 390-8621 Nagano, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Matsuda
authors.forename: Jun
authors.initials: J
authors.lastname: Miki
authors.forename: Yozo
authors.initials: Y
authors.lastname: Nakazawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakashita
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kamijo
authors.forename: Eiko
authors.initials: E
authors.lastname: Hidaka
authors.forename: Kenichi
authors.initials: K
authors.lastname: Koike
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation; D019070:Cell Lineage; D002675:Child, Preschool; D018450:Disease Progression; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D013601:T-Lymphocytes / Q000166:cytology*