[Effects of RGD tripeptide on the expression of FAK and HA in glomerular mesangial cells].
OBJECTIVE
Observing the effects of Arg-Gly-Asp (RGD) peptides on the proliferation of glomerular mesangial cells (GMCs), and on the expression of focal adheshion kinase (FAK) and hyaluronic acid (HA) in GMCs.


METHODS
GMCs of rats were induced with RGD peptide in vitro. The proliferation of GMCs was measured by MTT; the expression of FAK, by immunohistochemistry; and the quantity of HA, by radioimmunoassay.


RESULTS
After being incubated with RGD peptides, some of the attached GMCs became round, detached, and floated. As compared with the control, the proliferation (A value) of the induced with RGD peptide GMCs was significantly lower. After the cultured GMCs had been induced with RGD (1 mmol/L) for 24 h, the expression of FAK decreased by 3.10%, and the secretion of HA decreased by 5.64%. After the cultured GMCs had been incubated with RGD (4 mmol/L) for 8 h and 24 h, the expression of FAK decreased by 6.50% and 15.95%, and the secretion of HA decreased by 6.37% and 18.43%, respectively.


CONCLUSIONS
RGD peptides can inhibit the attachment of the cultured GMCs, reduce the proliferation of GMCs and decrease the secretion of the extracellular matrix.
Xiao
Li
L
Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
Mi
Xu-hua
XH
Huang
Song-min
SM
Tan
Hong
H
Liu
Xiao-jing
XJ
Zhou
Hai-yan
HY
Zhou
Li
L
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D051417:Focal Adhesion Kinase 1 / Q000096:biosynthesis* / Q000235:genetics; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D009842:Oligopeptides; D010455:Peptides; C047981:arginyl-glycyl-aspartic acid; D006820:Hyaluronic Acid; D051417:Focal Adhesion Kinase 1