title: Hemodynamic conditions alter axial and circumferential remodeling of arteries engineered ex vivo.
abstract: We previously demonstrated that growth and remodeling was stimulated in arteries elongated ex vivo using step increases in axial strain. Viability and vasoactivity were similar to fresh arteries, however there was a substantial decrease in the ultimate circumferential stress. To test the hypothesis that the subphysiological perfusion conditions (i.e., low pressure and flow) previously used caused the reduction, arteries were subjected to the identical elongation protocol (50% increase over 9 days) while being perfused with physiological levels of flow, viscosity and pulsatile pressure. A significant increase in unloaded length was achieved by elongation under both perfusion conditions, although the increase was less under physiological (7 +/- 1%) than under subphysiological conditions (19 +/- 2%, p < 0.005). When length at physiological stress was estimated using mechanical testing data the values were similar. The ultimate circumferential stress of arteries elongated under physiological conditions was increased (33%), whereas the ultimate axial stress was decreased (50%) as compared with arteries elongated under subphysiological conditions. Elongated arteries under both perfusion conditions showed significant increases in proliferation and collagen mass, and similar viability and appearance to fresh arteries. These data suggest that there is substantial cross-talk between perfusion conditions and axial strain that modulates arterial remodeling and length.
authors.affiliation: Department of Bioengineering, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Jason W
authors.initials: JW
authors.lastname: Nichol
authors.forename: Matus
authors.initials: M
authors.lastname: Petko
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Myung
authors.forename: J William
authors.initials: JW
authors.lastname: Gaynor
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Gooch
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000166:cytology / Q000254:growth & development*; D049109:Cell Proliferation; D003094:Collagen / Q000096:biosynthesis; D009924:Organ Culture Techniques; D010477:Perfusion / Q000379:methods; D013314:Stress, Mechanical; D013552:Swine; D023822:Tissue Engineering / Q000379:methods
chemical_list: D003094:Collagen