Molecular characterization and chromosomal mapping of porcine adipose differentiation-related protein (ADRP).
ADRP plays an important role in regulating lipid storage in various cells. We investigated the ADRP gene as a candidate gene for intramuscular fat deposition and marbling traits in pigs. A full-length transcript of porcine ADRP was cloned by RT-PCR and RACE. The porcine ADRP cDNA (1848 bp) contains a 1377-bp open reading frame, encoding a deduced protein of 459 amino acids, which has amino acid sequence identities of 89, 89, 82 and 81% with cattle, human, mouse and rat ADRP genes respectively. The genomic structure and sequence of the porcine ADRP were also analysed using a BAC clone of a Korean native pig. Pig ADRP comprises eight exons spanning approximately 13 kb and is located on chromosome 1 q2.3-q2.7 between microsatellite markers SW2185 and SW974. Several sequence variations were detected from nine different pig breeds. The biological role of this gene and the mapping localization indicated that the porcine ADRP is a possible candidate gene for fat deposition and marbling traits.
Kim
T H
TH
Animal Genomics and Bioinformatics Division, National Livestock Research Institute, Rural Development Administration, Suwon, Korea. kth6160@rda.go.kr
Choi
B H
BH
Chang
G W
GW
Lee
K T
KT
Lee
H Y
HY
Lee
J H
JH
Kim
K S
KS
Park
C K
CK
Moran
C
C
D000273:Adipose Tissue / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001823:Body Composition / Q000235:genetics; D002874:Chromosome Mapping / Q000662:veterinary; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D005787:Gene Frequency; D050356:Lipid Metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D000070780:Perilipin-2; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D013552:Swine / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry
D018076:DNA, Complementary; D008565:Membrane Proteins; C000602982:PLIN2 protein, human; D000070780:Perilipin-2; C000602983:Plin2 protein, mouse; C000602984:Plin2 protein, rat; D014157:Transcription Factors; D012313:RNA