[Expression of C II TA gene in five human malignant hematological cell lines and its significance].
To investigate the role of MHC class II Transactivator (C II TA) in expression of HLA molecules in five human malignant hematological cell lines.



The expressions of HLA molecules and C II TA protein were detected by immunohistochemistry and flow cytometry. The expression of C II TA gene was detected by RT-PCR. The response of peripheral T cells after stimulation by Jurkat cells was detected by mixed lymphocyte reaction.



The HLA II-positive tumor cells expressed the C II TA and IFN-gamma induced the expression of HLA I, II in tumor cells, which were able to express C II TA constitutively.



There is a correlation between the inability of the tumor cells in response to IFN-gamma for HLA expression and the deficiency in the inducible expression of C II TA.
Wei
Guo-qing
GQ
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Lin
Mao-fang
MF
D045744:Cell Line, Tumor; D005802:Genes, MHC Class II / Q000235:genetics*; D018922:HL-60 Cells; D006680:HLA Antigens / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics; D020298:U937 Cells
D006680:HLA Antigens; C083728:MHC class II transactivator protein; D009687:Nuclear Proteins; D015534:Trans-Activators