title: A new RNA synthetic method with a 2'-O-(2-cyanoethoxymethyl) protecting group.
abstract: A novel method for the synthesis of RNA oligomers with 2-cyanoethoxymethyl (CEM) as the 2'-hydroxyl protecting group has been developed. The new method allows the synthesis of oligoribonucleotides with an efficiency and final purity comparable to that obtained in DNA synthesis. [structure: see text]
authors.affiliation: Discovery Research Laboratories, Nippon Shinyaku Co. Ltd., 3-14-1 Sakura, Tsukuba City, Ibaraki 305-0003, Japan. t.ohgi@po.nippon-shinyaku.co.jp
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Ohgi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Masutomi
authors.forename: Kouichi
authors.initials: K
authors.lastname: Ishiyama
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kitagawa
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Shiba
authors.forename: Junichi
authors.initials: J
authors.lastname: Yano
mesh_terms: D001483:Base Sequence; D008956:Models, Chemical; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009570:Nitriles / Q000737:chemistry*; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D009570:Nitriles; D012313:RNA