Portable in situ fluorescence cytometry of microscale cell-based assays.
A portable fluorescence cytometric system has been developed for characterizing chemical concentration and cellular status in microscale cell culture analog (microCCA) devices. Based on discrete optical components, the system provides a modular platform for real-time image measurements applicable to a variety of cell-based microassays. As a feasibility study, we investigated the real-time dynamics of daunorubicin uptake with cultured mouse L-cells in a microCCA compartment. Time course results measured by the portable fluorescence cytometric system confirmed that in the microCCA devices daunorubicin accumulation is proportional to the liquid turnover rate.
Tatosian
Daniel A
DA
School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14850, USA.
Shuler
Michael L
ML
Kim
Donghyun
D
D000818:Animals; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D002460:Cell Line; D003630:Daunorubicin / Q000493:pharmacokinetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D005347:Fibroblasts / Q000378:metabolism*; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization
D003630:Daunorubicin