Expression of aquaporin-1 immunoreactivity by photoreceptor cells in the mouse retina.
Aquaporin water channels play a crucial role in the maintenance of ionic and osmotic homeostasis in the neural tissue. In the sensory retina, aquaporin-4 is expressed by Müller glial cells, predominantly in the inner retina, while aquaporin-1 is expressed mainly in the outer retina. However, it is unknown whether aquaporin-1 expression occurs in Müller cells or photoreceptor cells. By using immunohistochemical staining of retinal slices from rds mice, we show that the immunoreactivity for aquaporin-1 disappears along with the photoreceptor cell degeneration. In suspensions of dissociated retinal cells from control mice, photoreceptor cells expressed aquaporin-1 immunoreactivity while Müller cells were largely devoid of staining. The data suggest that photoreceptor cells, but not Müller cells, express aquaporin-1 in the murine retina.
Iandiev
Ianors
I
Department of Ophthalmology and Eye Clinic, University of Leipzig Medical Faculty, D-04103 Leipzig, Germany.
Pannicke
Thomas
T
Reichel
Martin B
MB
Wiedemann
Peter
P
Reichenbach
Andreas
A
Bringmann
Andreas
A
D000818:Animals; D000906:Antibodies; D051398:Aquaporin 1; D020346:Aquaporins / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains; D009419:Nerve Tissue Proteins / Q000235:genetics; D009457:Neuroglia / Q000378:metabolism; D064531:Peripherins; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism*; D012162:Retinal Degeneration / Q000235:genetics / Q000378:metabolism*; D014867:Water / Q000378:metabolism; D014882:Water-Electrolyte Balance / Q000502:physiology
D000906:Antibodies; C496021:Aqp1 protein, mouse; D020346:Aquaporins; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D064531:Peripherins; C578996:Prph2 protein, mouse; D014867:Water; D051398:Aquaporin 1