Antigens from Ascaris suum trigger in vitro macrophage NO production.
SUMMARY We investigated the in vitro effect of total excretory/secretory and somatic antigens from Ascaris suum adults (ESA and SA) and larvae 3 (ESL3 and SL3), and of 10 purified protein fractions from ESA components on rat alveolar macrophage nitric oxide (NO) production. Our results showed that in vitro incubation of macrophages with SA and SL3 antigens of A. suum did not result in NO release from cells, whereas incubation with ESA or ESL3 antigens resulted in the stimulation of NO production by these cells, both in a specific (inhibited by L-NAME and L-canavanine) and dose-dependent manner. In addition, we could demonstrate that a purified ESA fraction consisting of three Coomassie-stained bands of approximately 37, 44 and 46 kDa is involved in the in vitro triggering of NO production by host cells. These three bands were subjected to MALDI-peptide mass fingerprint, showing similarities with phosphoglycerate kinase, elongation factor Tu and enolase molecules, respectively. Future studies will focus on the characterization of these parasite-derived molecules.
Andrade
M Amparo
MA
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Salamanca, Spain.
Siles-Lucas
Mar
M
López-Abán
Julio
J
Carranza
Cristina
C
Pérez-Arellano
José L
JL
Muro
Antonio
A
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001196:Ascariasis / Q000276:immunology*; D017165:Ascaris suum / Q000276:immunology*; D002172:Canavanine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology*; D008297:Male; D008970:Molecular Weight; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis / Q000276:immunology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D009573:Nitrites / Q000032:analysis; D010449:Peptide Mapping; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000947:Antigens, Helminth; D004791:Enzyme Inhibitors; D009573:Nitrites; D009569:Nitric Oxide; D002172:Canavanine; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester