Identification of food-derived collagen peptides in human blood after oral ingestion of gelatin hydrolysates.
In the present study, we identified several food-derived collagen peptides in human blood after oral ingestion of some gelatin hydrolysates. Healthy human volunteers ingested the gelatin hydrolysates (9.4-23 g) from porcine skin, chicken feet, and cartilage after 12 h of fasting. Negligible amounts of the peptide form of hydroxyproline (Hyp) were observed in human blood before the ingestion. After the oral ingestion, the peptide form of Hyp significantly increased and reached a maximum level (20-60 nmol/mL of plasma) after 1-2 h and then decreased to half of the maximum level at 4 h after the ingestion. Major constituents of food-derived collagen peptides in human serum and plasma were identified as Pro-Hyp. In addition, small but significant amounts of Ala-Hyp, Ala-Hyp-Gly, Pro-Hyp-Gly, Leu-Hyp, Ile-Hyp, and Phe-Hyp were contained.
Iwai
Koji
K
Department of Food Sciences and Nutritional Health, Kyoto Prefectural University, 1-5 Shimogamo Kyoto 606-8522, Japan.
Hasegawa
Takanori
T
Taguchi
Yasuki
Y
Morimatsu
Fumiki
F
Sato
Kenji
K
Nakamura
Yasushi
Y
Higashi
Akane
A
Kido
Yasuhiro
Y
Nakabo
Yukihiro
Y
Ohtsuki
Kozo
K
D000328:Adult; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000097:blood*; D004044:Dietary Proteins / Q000008:administration & dosage*; D005780:Gelatin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006801:Humans; D010455:Peptides / Q000097:blood*; D011492:Protein Hydrolysates / Q000008:administration & dosage* / Q000493:pharmacokinetics; D013552:Swine
D004044:Dietary Proteins; D010455:Peptides; D011492:Protein Hydrolysates; D005780:Gelatin; D003094:Collagen