title: Urinary high performance reverse phase chromatography cortisol and cortisone analyses before and at the end of a race in elite cyclists.
abstract: A functional and basic method for the quantitative analysis of urine cortisol (F) and cortisone (E) using a Solid-Phase Extraction column and HPLC with ultraviolet detection is here described and validated to analyse urine samples. Urine specimens were analysed to study F and E relation and ratio in athletes and healthy sedentary subjects. The F and E concentrations in random urine specimens were significantly higher in the post exercise versus pre exercise condition in cyclists (F: 136+/-93 nmol/l versus 67+/-50 nmol/l (p<0.001); E: 797+/-400 nmol/l versus 408+/-252 nmol/l (p<0.001)). The F/E ratio was 0.18+/-0.11 versus 0.16+/-0.07, respectively, and a significant difference was only demonstrated comparing sedentary (0.11+/-0.07) and cyclist individuals at rest (p<0.05).
authors.affiliation: Section of Clinical Biochemistry, Department of Medical Diagnostic Sciences and Spec. Ther., University of Padua, 35100 Padua, Italy.
authors.forename: Rosalba
authors.initials: R
authors.lastname: Gatti
authors.forename: Enrico
authors.initials: E
authors.lastname: Cappellin
authors.forename: Barbara
authors.initials: B
authors.lastname: Zecchin
authors.forename: Giorgia
authors.initials: G
authors.lastname: Antonelli
authors.forename: Paolo
authors.initials: P
authors.lastname: Spinella
authors.forename: Franco
authors.initials: F
authors.lastname: Mantero
authors.forename: Elio Franco
authors.initials: EF
authors.lastname: De Palo
mesh_terms: D000293:Adolescent; D001642:Bicycling; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003348:Cortisone / Q000652:urine*; D006801:Humans; D006854:Hydrocortisone / Q000652:urine*; D008297:Male; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D003348:Cortisone; D006854:Hydrocortisone