title: Transferrin receptor-1 and 2 expression in chronic lymphocytic leukemia.
abstract: Transferrin receptor (TfR)-1 and 2 mRNA and CD71 (TfR1) expression was analyzed in 118 CLL patients. Ninety-five out of 109 analyzed cases expressed CD71, mostly at a high level; 60% of CD71 (+) cases were IGH-mutated. All samples were TfR1 mRNA (+); TfR2-alpha/beta mRNA was detected in, respectively, 52/102 and 100/109 cases. Competitive RT-PCR showed widely divergent levels of TfR1 mRNA in cases with high CD71 expression, alluding to post-transcriptional control of TfR1 expression in CLL. The almost uniformly high CD71 expression in CLL is in keeping with the activated status of neoplastic cells, regardless of IGH mutational load.
authors.affiliation: Laboratory of Molecular Genetics and Cytogenetics, University of Thessaly, Larissa, Greece.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Smilevska
authors.forename: Kostas
authors.initials: K
authors.lastname: Stamatopoulos
authors.forename: Maria
authors.initials: M
authors.lastname: Samara
authors.forename: Chrysoula
authors.initials: C
authors.lastname: Belessi
authors.forename: Aliki
authors.initials: A
authors.lastname: Tsompanakou
authors.forename: George
authors.initials: G
authors.lastname: Paterakis
authors.forename: Niki
authors.initials: N
authors.lastname: Stavroyianni
authors.forename: Ioanna
authors.initials: I
authors.lastname: Athanasiadou
authors.forename: Ioanna
authors.initials: I
authors.lastname: Chiotoglou
authors.forename: Anastasia
authors.initials: A
authors.lastname: Hadzidimitriou
authors.forename: Anastasia
authors.initials: A
authors.lastname: Athanasiadou
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Douka
authors.forename: Riad
authors.initials: R
authors.lastname: Saloum
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Laoutaris
authors.forename: Achilles
authors.initials: A
authors.lastname: Anagnostopoulos
authors.forename: Athanasios
authors.initials: A
authors.lastname: Fassas
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Stathakis
authors.forename: Panagoula
authors.initials: P
authors.lastname: Kollia
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000032:analysis; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis / Q000235:genetics*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D016130:Immunophenotyping; D037181:Lectins, C-Type; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D012333:RNA, Messenger / Q000032:analysis; D011990:Receptors, Transferrin / Q000032:analysis / Q000235:genetics*; D000099192:CD69 Antigens
chemical_list: D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D007143:Immunoglobulin Heavy Chains; D037181:Lectins, C-Type; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D000099192:CD69 Antigens; C068126:CD71 antigen; C503640:TFR2 protein, human