Mutational analysis of 9 different tumour-associated genes in human malignant mesothelioma cell lines.
Seven tumour suppressor genes (Chk1, Chk2, Apaf1, Rb1, p53, p16(INK4a) and p14(ARF)) and two oncogenes (N-ras and BRAF) were screened in nine human malignant melanoma (HMM) cell lines for point mutations or small deletions/insertions by DGGE, TGGE and SCCP analysis. For the first time in human mesothelioma, Chk1 gene mutations were detected in two of the nine investigated HMM cell lines. P53 gene mutations were found in three cell lines and p16(INK4a) mutations in 5. Mutation of the Chk1 gene implies a novel disruption mechanism of the p53 pathway in HMM, without affecting p53 itself. According to our knowledge, this is the first mutation screening of Chk1, Chk2, Apaf1 and Rb1 in human malignant mesothelioma.
Kumar
Krishan
K
University of Rostock, Department of Biological Sciences, Institute of Cell Biology and Biosystem Technology, Albert Einstein Strasse 3, D-18051 Rostock, Germany.
Rahman
Qamar
Q
Schipper
Holger
H
Matschegewski
Claudia
C
Schiffmann
Dietmar
D
Papp
Thilo
T
D001483:Base Sequence; D045744:Cell Line, Tumor; D000071877:Checkpoint Kinase 1; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D004252:DNA Mutational Analysis; D006801:Humans; D008654:Mesothelioma / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D015513:Oncogene Proteins / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D019941:Cyclin-Dependent Kinase Inhibitor p16; D015513:Oncogene Proteins; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D011494:Protein Kinases; C000605867:CHEK1 protein, human; D000071877:Checkpoint Kinase 1