Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation.
The Arabidopsis thaliana metal tolerance protein 1 (MTP1) of the cation diffusion facilitator family of membrane transport proteins can mediate the detoxification of Zn in Arabidopsis and yeast. Xenopus laevis oocytes expressing AtMTP1 accumulate more Zn than oocytes expressing the AtMTP1(D94A) mutant or water-injected oocytes. An AtMTP1-GFP fusion protein localizes to the vacuolar membrane in root and leaf cells. The analysis of Arabidopsis transformed with a promoter-GUS construct suggests that AtMTP1 is not produced throughout the plant, but primarily in the subpopulation of dividing, differentiating and expanding cells. RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues.
Desbrosses-Fonrouge
Anne-Garlonn
AG
Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Golm, Germany.
Voigt
Katrin
K
Schröder
Astrid
A
Arrivault
Stéphanie
S
Thomine
Sébastien
S
Krämer
Ute
U
D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D007425:Intracellular Membranes / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014617:Vacuoles / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism* / Q000633:toxicity
D029681:Arabidopsis Proteins; D027682:Cation Transport Proteins; D017931:DNA Primers; D015032:Zinc; C500829:ZAT protein, Arabidopsis