The effects of synthetic salmon calcitonin on thyroid C and follicular cells in adult female rats.
Structural and morphometric features of thyroid C and follicular cells were studied in adult rat females after treatment with synthetic salmon calcitonin (CT). The animals were chronically treated with either a low (10 IU/kg b.w) or a high (100 IU/kg b.w) dose of CT. A stereological method was applied to determine the volume density and the number of immunoreactive C cells. The height and volume density of follicular epithelium, colloid, interstitium and the follicles (epithelium plus colloid), as well as the index of activation rate were calculated. A significant decrease in body weight, as well as the volume density of immunoreactive C cells and the number of C cells per mm2, was observed in rats treated with both doses of CT. The height and volume density of follicular epithelium and follicles, as well as the index of activation rate were significantly increased in the animals given the high CT dose, while the volume densities of colloid and interstitium were reduced. No significant changes in the examined morphometric parameters were detected after treatment with the low CT dose. According to these results it can be concluded that the structural features of thyroid C and follicular cells were affected by the high dose CT treatment in the opposite manner, while the low dose CT treatment influenced only C cells.
Sekulić
M
M
Institute for Biological Research Sinisa Stanković, Belgrade, Serbia and Montenegro. sekmic@ibiss.bg.ac.yu
Sosić-Jurjević
B
B
Filipović
B
B
Milosević
V
V
Nestorović
N
N
Manojlović-Stojanoski
M
M
D000818:Animals; D002116:Calcitonin / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007150:Immunohistochemistry; D051381:Rats; D017208:Rats, Wistar; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure
C028815:salmon calcitonin; D002116:Calcitonin