Production of human caseinomacropeptide in recombinant Saccharomyces cerevisiae and Pichia pastoris.
Caseinomacropeptide is a polypeptide of 64 amino acid residues (106-169) derived from the C-terminal part of the mammalian milk k-casein. This macropeptide has various biological activities and is used as a functional food ingredient as well as a pharmaceutical compound. The gene encoding the human caseinomacropeptide (hCMP) was synthesized and expressed with an alpha-factor secretion signal in the two yeast strains, Saccharomyces cerevisiae and Pichia pastoris. The complete polypeptide of the recombinant hCMP was produced and secreted in a culture medium by both the strains, but the highest production was observed in S. cerevisiae with a galactose-inducible promoter. In a fed-batch bioreactor culture, 2.5 g/l of the recombinant hCMP was obtained from the S. cerevisiae at 97 h.
Kim
Yu-Jin
YJ
Department of Chemical Engineering, Pusan National University, 30 Jangjeon-dong, Geumjeong-gu, Busan, 609-735, Republic of Korea.
Oh
You-Kwan
YK
Kang
Whankoo
W
Lee
Eun Yeol
EY
Park
Sunghoon
S
D018533:Biomass; D019149:Bioreactors; D002364:Caseins / Q000096:biosynthesis* / Q000235:genetics; D005285:Fermentation; D005656:Fungal Proteins / Q000032:analysis / Q000302:isolation & purification; D005690:Galactose; D005813:Genes, Synthetic; D005822:Genetic Vectors; D006801:Humans; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000235:genetics; D010843:Pichia / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D002364:Caseins; D005656:Fungal Proteins; D010446:Peptide Fragments; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C065989:caseinomacropeptide; D005690:Galactose