Relation between insulin resistance and fast-migrating LDL subfraction as characterized by capillary isotachophoresis.
The proportion of the electronegative low density lipoprotein [LDL(-)] subfraction, which is atherogenic, is increased in type 2 diabetes but is not reduced by glycemic control. Therefore, we evaluated the ability of a new technique, capillary isotachophoresis (cITP), to quantify charge-based LDL subfractions and examined the relation between insulin resistance and the cITP fast-migrating (f) LDL levels. Seventy-five 10-year-old boys were included. The two cITP LDL subfractions, fLDL and major LDL subfractions, were proportional to the LDL protein content within the range of 0.1-0.8 mg/ml LDL protein. Levels of cITP fLDL were positively correlated with triglyceride (TG) levels and negatively correlated with LDL size. Insulin resistance as assessed by the homeostasis model assessment (HOMA-IR) was positively correlated (P < 0.01) with cITP fLDL levels (r = 0.41). The relation between HOMA-IR and cITP fLDL levels depended on TG levels but was independent of body mass index and LDL size. cITP lipoprotein analysis is an accurate and sensitive method for quantifying charge-based LDL subfractions in human plasma, and insulin resistance is related to cITP fLDL independent of LDL size.
Zhang
Bo
B
Department of Cardiology, Fukuoka University School of Medicine, Fukuoka, Japan. bozhang @fukuoka-u.ac.jp
Kaneshi
Takuya
T
Ohta
Takao
T
Saku
Keijiro
K
D000368:Aged; D015992:Body Mass Index; D002648:Child; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood; D015228:Hypertriglyceridemia / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology*; D008074:Lipoproteins / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification*; D008297:Male
D008074:Lipoproteins; D008077:Lipoproteins, LDL