title: Recognition of host immune activation by Pseudomonas aeruginosa.
abstract: It is generally reasoned that lethal infections caused by opportunistic pathogens develop permissively by invading a host that is both physiologically stressed and immunologically compromised. However, an alternative hypothesis might be that opportunistic pathogens actively sense alterations in host immune function and respond by enhancing their virulence phenotype. We demonstrate that interferon-gamma binds to an outer membrane protein in Pseudomonas aeruginosa, OprF, resulting in the expression of a quorum-sensing dependent virulence determinant, the PA-I lectin. These observations provide details of the mechanisms by which prokaryotic organisms are directly signaled by immune activation in their eukaryotic host.
authors.affiliation: Department of Surgery, University of Chicago, Pritzker School of Medicine, Chicago, IL 60637, USA.
authors.forename: Licheng
authors.initials: L
authors.lastname: Wu
authors.forename: Oscar
authors.initials: O
authors.lastname: Estrada
authors.forename: Olga
authors.initials: O
authors.lastname: Zaborina
authors.forename: Manjeet
authors.initials: M
authors.lastname: Bains
authors.forename: Le
authors.initials: L
authors.lastname: Shen
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Kohler
authors.forename: Nachiket
authors.initials: N
authors.lastname: Patel
authors.forename: Mark W
authors.initials: MW
authors.lastname: Musch
authors.forename: Eugene B
authors.initials: EB
authors.lastname: Chang
authors.forename: Yang-Xin
authors.initials: YX
authors.lastname: Fu
authors.forename: Michael A
authors.initials: MA
authors.lastname: Jacobs
authors.forename: Michael I
authors.initials: MI
authors.lastname: Nishimura
authors.forename: Robert E W
authors.initials: RE
authors.lastname: Hancock
authors.forename: Jerrold R
authors.initials: JR
authors.lastname: Turner
authors.forename: John C
authors.initials: JC
authors.lastname: Alverdy
mesh_terms: D018829:Adhesins, Bacterial / Q000096:biosynthesis*; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D037102:Lectins / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D018272:Porins / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity*; D011710:Pyocyanine / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial; D016207:Cytokines; D037102:Lectins; D018272:Porins; D011994:Recombinant Proteins; C038692:adhesin, Pseudomonas; D007371:Interferon-gamma; D011710:Pyocyanine