The use of acid phosphatase test papers for DNA profiling.
The acid phosphatase (AP) test is a routine assay used to screen casework items for the possible presence of semen. This colour test is carried out on filter paper which is retained after testing. Two-year-old AP test papers were found to contain sufficient DNA for short tandem repeat (STR) profiling. Prior to polymerase chain reaction (PCR) amplification, the DNA was preferentially separated into sperm depleted and sperm enriched cell fractions. The implication of these findings for past and present cases is discussed.
Reshef
A
A
Forensic Biology Laboratory, Israel Police, Israel Police National HQ, Jerusalem, 91906 Israel.
Barash
M
M
Gallili
N
N
Michael
A
A
Brauner
P
P
D000135:Acid Phosphatase / Q000032:analysis*; D000293:Adolescent; D004796:Clinical Enzyme Tests / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008297:Male; D010209:Paper / Q000592:standards*; D012661:Semen / Q000201:enzymology*; D020080:Tandem Repeat Sequences; D013997:Time Factors
D000135:Acid Phosphatase