Nucleoside-specific tolerance suppresses anti-nucleoside antibody forming cells.
Since its original development by Jerne, the haemolytic plaque assay has increased our understanding of antibody formation to a wide variety of antigens, including proteins, lipopolysaccharides, and simple haptens. We have now developed an assay to detect plaque forming cells (PFC) making anti-nucleoside antibodies. Previously we reported the suppression of circulating antibody to DNA determinants by nucleoside-IgG conjugates. Here we show that BALB/c mice can be rendered tolerant in terms of both direct and indirect anti-nucleoside antibody forming cells and that the state of tolerance is nucleoside-specific at the cellular level.
Stollar
B D
BD
Department of Biochemistry and Pharmacology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Borel
Y
Y
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology*; D000918:Antibody Specificity; D007108:Immune Tolerance / Q000276:immunology*; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009705:Nucleosides / Q000737:chemistry / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000906:Antibodies; D007074:Immunoglobulin G; D009705:Nucleosides