[Comparison of ALS gene mRNA between sessile and planktonic Candida albicans of oral cavities].
OBJECTIVE
To observe the different mRNA levels of Candida albicans ALS gene family between planktonic and biofilm-grown cells.


METHODS
ATCC 90038 and a wild strain of Candida albicans, biofilm models in vitro were formed on glass slides. After 48 hours' incubation, the biofilm-grown cells were harvested. Half-quantification of ALS1 and ALS4 mRNA was based on the amplification by one-step RT-PCR.


RESULTS
The amounts of ALS1 and ALS4 mRNA of the wild strain in biofilm increased comparing with planktonic cells, while ATCC 90038 didn't.


CONCLUSIONS
The members of ALS gene family may play important roles in the course of Candida albicans biofilm formation.
Qi
Qing-Gao
QG
Laboratory of Oral Biomedical Engineering, Ministry of Education, Sichuan University, Chengdu 610041, China.
Hu
Tao
T
Fu
Chu-Hua
CH
Zhou
Xue-Dong
XD
D018441:Biofilms; D002176:Candida albicans; D005656:Fungal Proteins; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D012333:RNA, Messenger
D005656:Fungal Proteins; D012333:RNA, Messenger