Ex vivo stimulation of cord blood mononuclear cells by dexamethasone and interleukin-7 results in the maturation of interferon-gamma-secreting effector memory T cells.
The effects of dexamethasone phosphate and interleukin-7 upon the proliferation of T-cells and the production of interferon-gamma in the newborn's cord blood mononuclear cell cultures were studied. The capability of dexamethasone to enhance T-cell proliferation induced by anti-CD3 with interleukin-7 in some newborn cord blood mononuclear cell cultures was identified. Dexamethasone suppressed production of interferon-gamma in 68-h cell cultures stimulated with anti-CD3 both in the presence of interleukin-7 and without it. However, a 68-h cultivation of newborn blood cells with dexamethasone, anti-CD3 and interleukin-7 resulted in the accumulation of T-lymphocytes capable of producing interferon-gamma after restimulation. As a result of it the amount of interferon-gamma producing CD7(+) T-cells and the concentration of interferon-gamma in cultural supernatants were maximal in the cell cultures incubated with anti-CD3, interleukin-7 and dexamethasone during the first 68 h and subsequently restimulated with phorbol 12-myristate 13-acetate and ionomycin. The stimulation of neonatal or adult blood cells by dexamethasone, anti-CD3 and interleukine-7 also causes a decrease in the number of naïve T-cells and central memory cells and an increase in the number of effector memory CD7(+)CD45RA(+)CD62L(-) cells in cultures. It is possible that these effects are caused by the influence of dexamethasone on IL-7 receptor expression: it is known that IL-7 receptor alpha-chain gene is a glucocorticoid-inducible gene.
Talayev
V Yu
VY
I. N. Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology, Ministry of Health of Russian Federation, Nizhny Novgorod, Russia. talaev@inbox.ru
Zaichenko
I Ye
IY
Babaykina
O N
ON
Lomunova
M A
MA
Talayeva
E B
EB
Nikonova
M F
MF
D000328:Adult; D017209:Apoptosis; D049109:Cell Proliferation; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D005312:Fetal Blood / Q000276:immunology*; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism*; D015851:Interleukin-7 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D013268:Stimulation, Chemical; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*
D005938:Glucocorticoids; D015851:Interleukin-7; D003907:Dexamethasone; D007371:Interferon-gamma