title: Expression and tissue distribution of astacin-like squid metalloprotease (ALSM).
abstract: Astacin metalloprotease family members function in a wide variety of biologic events, including cell differentiation and morphogenesis during embryonic development and adult tissue differentiation. We previously isolated and characterized an astacin-like squid metalloprotease (ALSM). To elucidate the embryonic expression of ALSM, we performed immunohistochemical analysis with specific antibodies and examined the expression profiles of ALSM isoforms by in situ hybridization analysis. Tissue distribution and expression were also examined in adult spear squid. mRNA expression of ALSM isoforms I and III was first detected in newly hatched squid and was restricted to the liver. No mRNA signals were detected in other tissues even in adult squids. At the protein level, both isoforms were prominent in the liver of embryos and later in digestive organs of adult squid. Both isoforms were also detected in muscle tissues, including mantle and tentacle muscle. Staining for ALSM III was also identified in the iris and in tissues near the eye in squid embryos. However, no reactive bands were detected by immunoblotting of adult squid eyes. Thus, ALSM is initially expressed at the late stage of embryogenesis in spear squid, and expression is restricted to the liver. Thereafter, ALSM isoforms function in various tissues in an isoform-dependent manner.
authors.affiliation: Department of Chemistry, Faculty of Science and Technology, Sophia University, 102-8554, Tokyo, Japan. n-kanza@sophia.ac.jp
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kanzawa
authors.forename: Shuntaro
authors.initials: S
authors.lastname: Tatewaki
authors.forename: Ryousuke
authors.initials: R
authors.lastname: Watanabe
authors.forename: Ikuko
authors.initials: I
authors.lastname: Kunihisa
authors.forename: Haruka
authors.initials: H
authors.lastname: Iwahashi
authors.forename: Kaori
authors.initials: K
authors.lastname: Nakamura
authors.forename: Takahide
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D049832:Decapodiformes / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis; D008099:Liver / Q000737:chemistry*; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D000906:Antibodies; D007527:Isoenzymes; D012333:RNA, Messenger; D008666:Metalloendopeptidases; C071288:astacin