[Apoptosis of human oral epidermoid carcinoma KB cells and multidrug resistant KBv200 cells induced by matrine].
OBJECTIVE
To investigate the induction of apoptosis on human oral epidermoid carcinoma KB cells and multidrug resistant KBv200 cells by Matrine.


METHODS
MTT assay was used to investigate the inhibition ability of Matrine on the cells in vitro. Transmission electron microscope was used to observe the ultrastructure feature of cells. after treated by Matrine. Acridine orange (AO)/Ethidium bromide (EB) fluorescent staining and flow cytometry were used to observe apoptosis induced by Matrine. Flow cytometry was applied to study the effects of the drug on cell cycles of the cells.


RESULTS
When 0.50, 1.00, 1.50, 2.00 mg/ml of Matrine was used, the vital rates of KB and KBv200 cells were decreased according to Matrine's concentration. The IC50 concentrations of Matrine on KB and KBv200 cells were 1.35 mg/ml and 1.43 mg/ml individually. The results of AO/EB fluorescent staining and flow cytometry showed that Matrine could induce apoptosis of two kinds of cells. While observed by transmission electron microscope, there were more contraction of cells, condensation of nuclei, bubble of cytoplasm in both kinds of cells after treated by Matrine. Matrine could stop the growth of KB and KBv200 cells at S period and restrain mitosis of cells.


CONCLUSIONS
Matrine can inhibit the growth of KB and KBv200 cells by inducing apoptosis. The apoptosis effect is dose-dependent and it has certain relation to the blocking of S period cells.
Zhang
Jin-Ting
JT
Dept. of Oral Medicine, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Cui
Hui-Xian
HX
Li
Qing-Xing
QX
Zhang
Hui-Jun
HJ
Bai
Yu
Y
D000470:Alkaloids; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell; D006801:Humans; D007624:KB Cells; D011807:Quinolizines; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; D000093842:Matrines