Interactions among measles virus hemagglutinin, fusion protein and cell receptor signaling lymphocyte activation molecule (SLAM) indicating a new fusion-trimer model.
For measles viruses, fusion on the cell membrane is an important initial step in the entry into the infected cells. The recent research indicated that hemagglutinin firstly leads the conformational changes in the fusion protein then co-mediates the membrane fusion. In the work, we use the co-immunoprecipitation and pull-down techniques to identify the interactions among fusion protein, hemagglutinin and signaling lymphocyte activation molecule (SLAM), which reveal that the three proteins can form a functional complex to mediate the SLAM-dependent fusion. Moreover, under the confocal microscope, fusion protein and hemagglutinin protein can show the cocapping mediated by the SLAM. So fusion protein not only is involved in the fusion but also might directly interact with the SLAM to be a new fusion-trimer model, which might account for the infection mechanism of measles virus.
Zhang
Peng
P
National Key Lab of Virology, College of Life Scince, Wuhan University, Hu Bei, Wuhan 430072, P. R. China.
Li
Lingyun
L
Hu
Chunlin
C
Xu
Qin
Q
Liu
Xin
X
Qi
Yipeng
Y
D000818:Animals; D015703:Antigens, CD; D016466:CHO Cells; D006224:Cricetinae; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D008459:Measles virus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D008561:Membrane Fusion / Q000502:physiology*; D010957:Plasmids; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins / Q000235:genetics; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D015703:Antigens, CD; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D007136:Immunoglobulins; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1