Transplantation of cultured bone marrow stromal cells to improve peripheral nerve regeneration.
The role of cultured bone marrow stromal cells (BMSCs) in peripheral nerve regeneration was examined using an established rabbit peroneal nerve regeneration model. A 15-mm peroneal nerve defect was bridged with a vein filled with BMSCs (1 x 10(6)), which had been embedded in collagen gel. On the contralateral side, the defect was bridged with a vein filled with collagen gel alone. When the regenerated tissue was examined 4, 8 and 12 weeks after grafting, the number and diameter of the myelinated fibers in the side with the BMSCs were significantly higher than in the control side without the BMSCs. This demonstrates the potential of using cultured BMSCs in peripheral nerve regeneration.
Choi
B-H
BH
Department of Oral and Maxillofacial Surgery, College of Dentistry, Yonsei University (Brain Korea 21 Project for Medical Sciences), Seoul, South Korea. choibh@wonju.yonsei.ac.kr
Zhu
S-J
SJ
Kim
B-Y
BY
Huh
J-Y
JY
Lee
S-H
SH
Jung
J-H
JH
D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000627:therapeutic use; D005347:Fibroblasts / Q000502:physiology; D005782:Gels; D048091:Guided Tissue Regeneration / Q000295:instrumentation / Q000379:methods; D008297:Male; D009413:Nerve Fibers, Myelinated / Q000502:physiology / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology*; D016900:Neurofilament Proteins / Q000032:analysis; D010543:Peroneal Nerve / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D011817:Rabbits; D009418:S100 Proteins / Q000032:analysis; D017154:Stromal Cells / Q000637:transplantation
D005782:Gels; D016900:Neurofilament Proteins; D009418:S100 Proteins; C061195:neurofilament protein M; D003094:Collagen; D010751:Phosphopyruvate Hydratase