title: Genetic effects of anorganic bovine bone (Bio-Oss) on osteoblast-like MG63 cells.
abstract: Bio-Oss (Geistlich, Wolhusen, Switzerland) is composed by anorganic bovine bone and is widely used in several bone regeneration procedures in oral surgery. How this biomaterial alters osteoblast gene expression to promote bone formation is poorly understood. We therefore attempted to address this question by using microarray techniques to identify genes that are differentially regulated in osteoblasts exposed to Bio-Oss. By using DNA microarrays containing 20,000 genes, we identified in osteoblast-like cells line (MG-63) cultured with Bio-Oss several genes which expression was significantly up- and down-regulated. The differentially expressed genes cover a broad range of functional activities: (a) signaling transduction, (b) transcription, (c) cell cycle regulation, (d) vesicular transport, (e) apoptosis, and (f) immunity. These results could explain the reported bioaffinity of Bio-Oss to host animals, its biological affinity to osteogenic cells and its capability to stimulate osteoblastic differentiation. The data reported are, to our knowledge, the first genetic portrait of Bio-Oss effects. They can be relevant to our improved understanding of the molecular mechanism underlying bone regenerative procedures and as a model for comparing other materials with similar clinical effects.
authors.affiliation: University of Ferrara, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Carinci
authors.forename: Adriano
authors.initials: A
authors.lastname: Piattelli
authors.forename: Marco
authors.initials: M
authors.lastname: Degidi
authors.forename: Annalisa
authors.initials: A
authors.lastname: Palmieri
authors.forename: Vittoria
authors.initials: V
authors.lastname: Perrotti
authors.forename: Luca
authors.initials: L
authors.lastname: Scapoli
authors.forename: Marcella
authors.initials: M
authors.lastname: Martinelli
authors.forename: Gregorio
authors.initials: G
authors.lastname: Laino
authors.forename: Furio
authors.initials: F
authors.lastname: Pezzetti
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects / Q000235:genetics; D018786:Bone Substitutes / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D002468:Cell Physiological Phenomena / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D020869:Gene Expression Profiling / Q000379:methods; D008903:Minerals / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D008903:Minerals