Acute respiratory infection with mouse adenovirus type 1.
Studies of the pathogenesis of adenovirus respiratory disease are limited by the strict species-specificity of the adenoviruses. Following intranasal inoculation of adult C57BL/6 mice with mouse adenovirus type 1 (MAV-1), we detected MAV-1 early region 3 (E3) and hexon gene expression in the lungs at 7 days post-infection (dpi). We detected MAV-1 E3 protein in the respiratory epithelium at 7 dpi. We did not detect viral mRNA or protein at 14 dpi, but MAV-1 DNA was detected by PCR at 21 dpi. Chemokine transcript levels increased between 7 and 14 dpi in the lungs of infected mice. MAV-1 infection induced a patchy cellular infiltrate in lungs at 7 and 14 dpi. This is the first report demonstrating the presence of MAV-1 in the respiratory epithelium of infected mice and describing chemokine responses in the lung induced by MAV-1 respiratory infection. MAV-1 infection of mice has the potential to serve as a model for inflammatory changes seen in human adenovirus respiratory disease.
Weinberg
Jason B
JB
University of Michigan Health System, Division of Pediatric Infectious Diseases, Department of Pediatrics, Ann Arbor, 48109-0244, USA. jbwein@umich.edu
Stempfle
Gregory S
GS
Wilkinson
John E
JE
Younger
John G
JG
Spindler
Katherine R
KR
D000818:Animals; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004195:Disease Models, Animal; D007249:Inflammation; D008168:Lung / Q000473:pathology / Q000821:virology; D017921:Mastadenovirus / Q000145:classification / Q000472:pathogenicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000473:pathology / Q000821:virology*
D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral