title: Microchip capillary gel electrophoresis using programmed field strength gradients for the ultra-fast analysis of genetically modified organisms in soybeans.
abstract: We have developed a novel method for the ultra-fast analysis of genetically modified organisms (GMOs) in soybeans by microchip capillary gel electrophoresis (MCGE) using programmed field strength gradients (PFSG) in a conventional glass double-T microchip. Under the programmed electric field strength and 0.3% poly(ethylene oxide) sieving matrix, the GMO in soybeans was analyzed within only 11 s of the microchip. The MCGE-PFSG method was a program that changes the electric field strength during GMO analysis, and was also applied to the ultra-fast analysis of PCR products. Compared to MCGE using a conventional and constantly applied electric field, the MCGE-PFSG analysis generated faster results without the loss of resolving power and reproducibility for specific DNA fragments (100- and 250-bp DNA) of GM-soybeans. The MCGE-PFSG technique may prove to be a new tool in the GMO analysis due to its speed, simplicity, and high efficiency.
authors.affiliation: Department of Chemistry, Chonbuk National University, Jeonju 561-756, South Korea.
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Kim
authors.forename: Joon-Seok
authors.initials: JS
authors.lastname: Chae
authors.forename: Jun Keun
authors.initials: JK
authors.lastname: Chang
authors.forename: Seong Ho
authors.initials: SH
authors.lastname: Kang
mesh_terms: D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013025:Glycine max / Q000235:genetics*
chemical_list: D018744:DNA, Plant