Deep venous thrombosis: behaviour of the polymorphonuclear leukocyte integrin pattern at baseline and after in vitro activation.
In a group of 18 subjects with acute deep venous thrombosis (DVT), evidenced by clinical examination and echo-color-Doppler, we examined the phenotypical expression of the polymorphonuclear leukocyte (PMN) beta2-integrins (CD11a, CD11b, CD11c, CD18), obtained by using a flow cytofluorimeter. The evaluation was performed before and after in vitro activation (prolonged for 5 and 15 minutes) with 4-phorbol 12-myristate 13-acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). In DVT subjects, at baseline, the phenotypical expression of CD11b was decreased and that of CD11c was increased when compared with normal controls; no difference was found in CD11a and CD18 expression. In normal subjects PMN activation with both activators led to a constant increase of all PMN adhesion molecules; in DVT subjects CD11b, CD11c and CD18 increased, while CD11a expression did not show any change. These data indicate the presence of a functional alteration in circulating PMN cells from patients with DVT.
Caimi
G
G
Dipartimento di Medicina Interna, Malattie Cardiovascolari e Nefrourologiche, Università di Palermo, Italy. caimigre@unipa.it
Tozzi Ciancarelli
M G
MG
Ferrara
F
F
Montana
M
M
Calandrino
V
V
Canino
B
B
Lo Presti
R
R
D000328:Adult; D000368:Aged; D018845:CD11 Antigens / Q000032:analysis / Q000235:genetics; D018821:CD18 Antigens / Q000032:analysis / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D016023:Integrins / Q000032:analysis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D018375:Neutrophil Activation; D009504:Neutrophils / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D020246:Venous Thrombosis / Q000097:blood*
D018845:CD11 Antigens; D018821:CD18 Antigens; D016023:Integrins; D013755:Tetradecanoylphorbol Acetate