An ELISA method for the identification of salivary amylase.
An ELISA method for the detection of salivary amylase in dried stains using a monoclonal anti-human salivary amylase antibody was developed. Studies demonstrated the assay to be sensitive down to 0.0002 Sigma units and showed a linear response between absorbance and salivary amylase activity between 0.002 and 0.2 units. The assay showed no cross reactivity with either commercially purchased human pancreatic or bacterial amylase. Sample studies utilizing swabs from several human body fluids showed that 100% of all saliva containing swabs (sixteen of sixteen) and 13% of non-saliva human body fluid swabs (eight of sixty-three) showed a net absorbance with the method. Of these eight non-saliva swabs yielding a net absorbance, none exceeded a salivary amylase activity of 0.003 units. In contrast, only three of the sixteen saliva-containing swabs (swabs produced from saliva diluted 1:5, 1:6, and 1:10, respectively) showed an activity below 0.2 units. Of these swabs, the 1:100 dilution showed the lowest activity (0.048). This value is still more than ten times that of the non-saliva containing swab with the highest activity.
Quarino
Lawrence
L
Office of Chief Medical Examiner, Department of Forensic Biology, New York City, USA.
Dang
Qui
Q
Hartmann
John
J
Moynihan
Nora
N
D000681:Amylases / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal; D001774:Blood Chemical Analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000201:enzymology; D005260:Female; D005554:Forensic Medicine; D005766:Gastrointestinal Contents / Q000201:enzymology; D006801:Humans; D008297:Male; D010179:Pancreas / Q000201:enzymology; D012463:Saliva / Q000201:enzymology*; D012661:Semen / Q000201:enzymology; D013542:Sweat / Q000201:enzymology; D014556:Urine / Q000737:chemistry; D014621:Vagina / Q000201:enzymology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000681:Amylases