Nucleolar localization of non-structural protein 3b, a protein specifically encoded by the severe acute respiratory syndrome coronavirus.
The open reading frame 3 (ORF3) of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted 154-amino acid protein, which lacks similarities to any known protein, and is named 3b. In this study, it was shown that 3b protein was predominately localized to nucleus with EGFP tag at its N- or C-terminus. The localization patterns were similar in different transfected cells. Immuno-fluorescence assay revealed that 3b protein was co-localized well with C23 in nucleolus. C23, B23 and fibrillarin all are important nucleolar proteins, which localize in the region of the nucleolus. Co-transfection of p3b-EGFP with pC23-DsRed, pB23-DsRed and pfibrillarin-DsRed further confirmed 3b's nucleolus localization. With construction of serial truncated mutants of 3b, a region (residues 134-154 aa) responsible for nucleolar localization was determinated in 3b protein. These results provide a new insight for further functional studies of SARS-CoV 3b protein.
Yuan
Xiaoling
X
Department of Pathophysiology, Beijing Institute of Radiation Medicine, No. 27 Taiping Road, Beijing 100850, China.
Yao
Zhenyu
Z
Shan
Yajun
Y
Chen
Bo
B
Yang
Zhen
Z
Wu
Jie
J
Zhao
Zhenhu
Z
Chen
Jiapei
J
Cong
Yuwen
Y
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000378:metabolism; D021382:Protein Sorting Signals; D016601:RNA-Binding Proteins / Q000378:metabolism; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D014162:Transfection; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000097796:Nucleolin
D002868:Chromosomal Proteins, Non-Histone; D049452:Green Fluorescent Proteins; D009687:Nuclear Proteins; D000090243:Nucleophosmin; D010750:Phosphoproteins; D021382:Protein Sorting Signals; D016601:RNA-Binding Proteins; D017361:Viral Nonstructural Proteins; D000097796:Nucleolin; C047458:fibrillarin; C118611:glycoprotein 3b, coronavirus