A colorimetric titration method for quantification of millimolar glucose in a pH 7.4 aqueous phosphate buffer.
3-Pyridinylboronic acid is identified as a key sensing element for reversible sugar complexation in an aqueous solution at the physiological pH. The utility of a sensing element has been demonstrated through a simple colorimetric titration of glucose using absorption spectroscopy in the visible region. The mechanism of the high diol/triol binding affinity of pyridine boronic acid in the neutral pH is discussed based on the 1H and 11B NMR spectroscopic studies.
Boduroglu
Serhan
S
Department of Chemistry, The University of Akron, Akron, OH 44325-3601, USA.
El Khoury
Jouliana M
JM
Venkat Reddy
D
D
Rinaldi
Peter L
PL
Hu
Jun
J
D001897:Boronic Acids; D002021:Buffers; D002138:Calibration; D003124:Colorimetry / Q000379:methods*; D005947:Glucose / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D013057:Spectrum Analysis; D017186:Titrimetry
D001897:Boronic Acids; D002021:Buffers; D005947:Glucose