Genotyping of hepatitis B virus isolated from chronic hepatitis B patients in the south of Turkey by DNA cycle-sequencing method.
The 8 genotypes of hepatitis B virus (HBV A-H) show a distinct geographic distribution and influence the course of disease and the prognosis of treatment. In this study, we have genotyped 50 HBV isolates circulating in the south of Turkey by DNA cycle sequencing, based on their compatibility with reference sequences of a part of S gene. In our cases, all 50 (100%) HBV sequences from the patients demonstrated full compatibility with the sequences of ayw subtype viruses in genotype D. However, we have found some nucleotide sequence variations within genotype D, 47 (94%) of which were related to HBVGEN1 (Z35716 genotype D) and 3 (6%) were related to HBVDNA (X68292, genotype D).
Serin
Mehmet S
MS
Department of Microbiology, Faculty of Pharmacy, University of Mersin, Mersin, Turkey 33169. serinmss@cu.edu.tr
Akkiz
Hikmet
H
Abayli
Bahri
B
Oksuz
Murat
M
Aslan
Gonul
G
Emekdas
Gurol
G
D004279:DNA, Viral / Q000032:analysis; D005838:Genotype; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014421:Turkey
D004279:DNA, Viral