[Therapeutic effect of Qingkailing and methylprednisolone injection alone or combined on the acute lung injury induced by oleic acid in rabbits].
OBJECTIVE
To investigate the effect of Qingkailing and Methylprednisolone (MP) injection alone or combined on the acute lung injury (ALI) induced by oleic acid in rabbits.


METHODS
The rabbits were randomly divided into 11 groups: oleic acid group; control group; treatment groups including low, middle and high dosage groups of Qingkailing and MP alone and combined, respectively. ALI model was established by i.v. oleic acid (0.05 mL x kg(-1)) in these groups, and then i.v. above drugs respectively, while in control group, the same volume of normal saline was given. The respiratory amplitude and rate were observed, and blood samples were taken from cervical artery for blood-gas analysis before and at 30, 60, 120 min after oleic acid or normal saline administration. At the end of experiment, the concentration of LDH, CAT and MDA in the lung tissue were measured and pathologic changes of lung tissue were observed microscopically.


RESULTS
Compared with oleic acid group, the respiratory amplitude markedly enhanced (P < 0.05) and respiratory rate lowered (P < 0.05) in the low, middle and high dose groups of Qingkailing and MP injection. On the 30 min of treatment, PaO2 increased significantly (P < 0.05) in the low and middle dose groups of combined Qingkailing and MP injection; PaCO2 decreased markedly (P < 0.05) on the 120 min of treatment in each treatment group. The level of LDH significantly increased (P < 0.05), CAT and MDA decreased (P < 0.05) in the middle and high groups of Qingkailing and MP injection. The low and middle dose groups of combined Qingkailing and MP injection can alleviate the pathological changes induced by oleic acid.


CONCLUSIONS
The curative effect of the low dose group of combined Qingkailing and MP for the ALI induced by oleic acid was better than Qingkailing and MP alone, while the big dose groups of Qingkailing and MP alone better than the combination at the same dosage.
Du
Gui-you
GY
Institute of Chinese Material Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China. duguiyou1942@vip.sina.com
Liang
Ri-xin
RX
Zhang
Chun-ying
CY
Wang
Xiu-rong
XR
Gao
Wei
W
Cui
Hai-feng
HF
Zhao
Yong
Y
Wu
Zi-lun
ZL
He
Rong
R
Gao
Shuang-rong
SR
Hui
Lian-qiang
LQ
Zhu
Xiao-xin
XX
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D001784:Blood Gas Analysis / Q000379:methods; D004359:Drug Therapy, Combination; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000627:therapeutic use*; D005260:Female; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D008775:Methylprednisolone / Q000627:therapeutic use*; D019301:Oleic Acid; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D011817:Rabbits; D011897:Random Allocation; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D012129:Respiratory Function Tests
D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D019301:Oleic Acid; D008775:Methylprednisolone