Product and product-independent induction of butane oxidation in Pseudomonas butanovora.
Pseudomonas butanovora grows on butane by means of an inducible soluble alkane monooxygenase (sBMO). The induction of sBMO was studied using the wild type and a sBMO reporter strain. The reporter strain has the lacZ::kan cassette inserted into bmoX, the gene that encodes the alpha-subunit of the hydroxylase of sBMO. The beta-galactosidase activity in the reporter strain was not induced by butane, but was induced by 1-butanol and butyraldehyde. P. butanovora expressed sBMO product-independent activity at 3.0+/-1 nmol ethylene oxide min(-1) mg protein(-1) in stationary phase. The sBMO product-independent activity likely primes the expression of sBMO by butane.
Sayavedra-Soto
Luis A
LA
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA. sayavedl@science.oregonstate.edu
Doughty
David M
DM
Kurth
Elizabeth G
EG
Bottomley
Peter J
PJ
Arp
Daniel J
DJ
D020001:1-Butanol / Q000378:metabolism / Q000494:pharmacology; D000447:Aldehydes / Q000378:metabolism / Q000494:pharmacology; D002073:Butanes / Q000378:metabolism* / Q000494:pharmacology; D042926:Cytochrome P-450 CYP4A / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D017930:Genes, Reporter; D007763:Lac Operon; D008954:Models, Biological; D016254:Mutagenesis, Insertional; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012995:Solubility
D000447:Aldehydes; D002073:Butanes; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; C046888:butane; D020001:1-Butanol; D042926:Cytochrome P-450 CYP4A; C018475:butyraldehyde