Targeted delivery of arjunglucoside I using surface hydrophilic and hydrophobic nanocarriers to combat experimental leishmaniasis.
The purpose of the present study was to investigate the therapeutic efficacy of the indigenous drug arjunglucoside I (AG) against in vivo models of experimental leishmaniasis by incorporating it in surface hydrophilic co-polymeric nanogel particles of size less than 100 nm diameter and to compare its efficacy with that of the free drug as well as the drug encapsulated in hydrophobic poly-dl-lactide (PLA) nanoparticles. The drug AG, having glucose at the terminal end of the glycosidic chain, was isolated from an indigenous source. Drug-incorporated ultra-low-sized nanogels (approximately 90 nm in diameter) composed of cross-linked random co-polymer of N-isopropylacrylamide (NIPAAM) and N-vinyl pyrrolidone(VP) were prepared, characterized and used as delivery vehicles to combat experimental leishmaniasis in hamster models. For comparison, drug-encapsulated hydrophobic nanoparticles (approximately 250 nm in diameter) made from PLA were used as a control. The drug AG was incorporated in these nanocarriers and these drug-nanocarrier complexes were physically characterized. The efficacy of lowering spleen parasite load by the free drug, as well as that incorporated in nanogels and PLA nanoparticles were examined in vivo in equimolar concentration against hamsters undergoing experimental leishmaniasis. The reduction of drug toxicity by the nanogels and PLA nanoparticles was also assessed. The efficacy in the lowering of spleen parasite load with the free drug was found to be only 38% but was much higher when the drug was incorporated in co-polymeric nanogels (79%) or in polymeric nanoparticles (75%). Both the nanocarriers were found to be effective in reducing hepatotoxicity and nephrotoxicity nearly to the same extent. It was apparent that in addition to a smaller size and better drug release profile, the contribution of other parameters, e.g. overall surface hydrophilicity or hydrophobicity of the vehicles, also play an important role in the macrophage uptake of the drug. However, whatever be the exact mechanism, being highly efficient, non-hepatotoxic and non-nephrotoxic, AG in either of the two nanoparticulate forms may have useful application in humans
Tyagi
Richa
R
Department of Chemistry, University of Delhi, Delhi, 110 007, India.
Lala
Sanchaita
S
Verma
Anita K
AK
Nandy
A K
AK
Mahato
Shashi Bhushan
SB
Maitra
Amarnath
A
Basu
Mukul Kumar
MK
D000818:Animals; D000977:Antiparasitic Agents / Q000008:administration & dosage* / Q000627:therapeutic use* / Q000633:toxicity; D001806:Blood Urea Nitrogen; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000473:pathology; D002626:Chemistry, Pharmaceutical; D003404:Creatinine / Q000097:blood; D006224:Cricetinae; D004337:Drug Carriers; D004798:Enzymes / Q000097:blood; D005260:Female; D005782:Gels; D005960:Glucosides / Q000008:administration & dosage* / Q000627:therapeutic use* / Q000633:toxicity; D007674:Kidney Diseases / Q000201:enzymology / Q000473:pathology; D019344:Lactic Acid; D007893:Leishmania donovani; D007898:Leishmaniasis, Visceral / Q000188:drug therapy* / Q000469:parasitology; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D009828:Oleanolic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use / Q000633:toxicity; D010316:Particle Size; D010592:Pharmaceutic Aids; D024301:Plant Bark / Q000737:chemistry; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D012542:Scattering, Radiation; D017550:Spectroscopy, Fourier Transform Infrared; D029766:Terminalia / Q000737:chemistry; D014961:X-Ray Diffraction
D000977:Antiparasitic Agents; D004337:Drug Carriers; D004798:Enzymes; D005782:Gels; D005960:Glucosides; D010592:Pharmaceutic Aids; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D009828:Oleanolic Acid; D003404:Creatinine; C479759:sericoside