title: Infection of human B lymphoma cells by Mycoplasma fermentans induces interaction of its elongation factor with the intracytoplasmic domain of Epstein-Barr virus receptor (gp140, EBV/C3dR, CR2, CD21).
abstract: Human cell lines are often infected by mycoplama strains. We have demonstrated that when infected by Mycoplasma fermentans, human B lymphoma cell proliferation increased strongly. These infected B cells expressed a p45 kDa protein which interacted with the intracellular domain of CD21, the EBV/C3d receptor. p45 analysis demonstrated that this is a new gene which encodes an elongation factor originating from Mycoplasma fermentans. p45 interaction with CD21 was specific, there being no interaction with CD19. This is the first demonstration that Mycoplasma fermentans, in infecting human B cells, generates a p45 Mycoplasma component that interacts with CD21, which is involved in B cell proliferation.
authors.affiliation: INSERM U.672 (ex U.354), Immunochimie des Régulations Cellulaires et des Interactions Virales, Bâtiment G8, Campus 1, Génopole d'Evry, 5 rue Henri Desbruères, 91030, EVRY Cedex, France.
authors.forename: Michelle
authors.initials: M
authors.lastname: Balbo
authors.forename: Monique
authors.initials: M
authors.lastname: Barel
authors.forename: Séverine
authors.initials: S
authors.lastname: Lottin-Divoux
authors.forename: Didier
authors.initials: D
authors.lastname: Jean
authors.forename: Raymond
authors.initials: R
authors.lastname: Frade
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000382:microbiology* / Q000473:pathology*; D002455:Cell Division; D045744:Cell Line, Tumor; D003062:Codon / Q000235:genetics; D003593:Cytoplasm / Q000378:metabolism; D017931:DNA Primers; D006801:Humans; D016393:Lymphoma, B-Cell / Q000382:microbiology* / Q000473:pathology*; D008969:Molecular Sequence Data; D009175:Mycoplasma Infections / Q000378:metabolism*; D016858:Mycoplasma fermentans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry; D016133:Polymerase Chain Reaction; D017464:Receptors, Complement 3d / Q000235:genetics / Q000378:metabolism*
chemical_list: D003062:Codon; D017931:DNA Primers; D010446:Peptide Fragments; D017464:Receptors, Complement 3d