Application of spectrofluorometry to the prediction of PHB concentrations in a fed-batch process.
On-line estimation of biopolymer production during fermentation would be a useful adjunct to the development of strategies for process control and optimization. This study examined the applicability of spectrofluorometry, along with other on-line measurements, for the prediction of poly-beta-hydroxybutyric acid (PHB) concentrations in a high-cell density fed-batch fermentation of Ralstonia eutropha. Models previously used for modelling PHB evolution with time are not sufficiently accurate for situations where transient intermediate accumulations or PHB degradation occur. Thus, the mass balance in the model was modified to account for these situations. An estimation algorithm was developed that is based on a hybrid model consisting of a dynamic mass balance of PHB where the main reaction coefficient was regressed with respect to spectrofluorometric data. The regression between the kinetic parameter and the spectrofluorometric data was accomplished using partial least squares (PLS) regression to avoid high sensitivity to noise expected from highly correlated data, such as the spectrofluorometric readings. The model accounts for dynamics of intermediates and in this way allows the prediction of dynamic behaviour in PHB concentrations that cannot be predicted with other reported mathematical models.
Henneke
Daniel
D
Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada, N2L 3G1.
Hagedorn
Andrea
A
Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada, N2L 3G1.
Budman
Hector M
HM
Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada, N2L 3G1.
Legge
Raymond L
RL
Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada, N2L 3G1. rllegge@engmail.uwaterloo.ca.
D000465:Algorithms; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D020118:Cupriavidus necator / Q000254:growth & development* / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D008954:Models, Biological; D009862:Online Systems; D011091:Polyesters / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D000094122:Polyhydroxybutyrates
D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates