Detection of myelination using a novel histological probe.
Current methods for myelin staining in tissue sections include both histological and immunohistochemical techniques. Fluorescence immunohistochemistry, which uses antibodies against myelin components such as myelin basic protein, is often used because of the convenience for multiple labeling. To facilitate studies on myelin, this paper describes a quick and easy method for direct myelin staining in rodent and human tissues using novel near-infrared myelin (NIM) dyes that are comparable to other well-characterized histochemical reagents. The near-infrared fluorescence spectra of these probes allow fluorescent staining of tissue sections in multiple channels using visible light fluorophores commonly used in immunocytochemistry. These dyes have been used successfully to detect normal myelin structure and myelin loss in a mouse model of demyelination disease.
Xiang
Zhongmin
Z
The MassGeneral Institute for Neurodegenerative Diseases (MIND), Charlestown, MA 02129-4404, USA.
Nesterov
Evgueni E
EE
Skoch
Jesse
J
Lin
Tong
T
Hyman
Bradley T
BT
Swager
Timothy M
TM
Bacskai
Brian J
BJ
Reeves
Steven A
SA
D000818:Animals; D001921:Brain / Q000378:metabolism; D003711:Demyelinating Diseases / Q000378:metabolism; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D019265:Spectroscopy, Near-Infrared
D005456:Fluorescent Dyes