Catecholamine-synthesizing enzymes in the adult and prenatal human testis.
Catecholamines play functional roles in the mature and developing mammalian testis but the cell types responsible for their local synthesis are still controversially discussed. Here, we demonstrate that four enzymes involved in the biosynthesis of catecholamines, namely, tyrosine hydroxylase (TH), aromatic amino acid decarboxylase (AADC), dopamine beta-hydroxylase (DBH) and phenylethanolamine- N-methyltransferase (PNMT), are expressed in Leydig cells of the human testis. Tyrosine hydroxylase, the key enzyme of the biosynthesis of catecholamines, was localized to Leydig cells both at the transcript level (by RT-PCR analyses and by in situ hybridization assays) and at the protein level (by immunoblotting and by immunohistochemistry). The other enzymes were also demonstrated in Leydig cells by RT-PCR and immunohistochemical analyses. The presence of TH, AADC, DBH, and PNMT in human Leydig cells was found, in addition, by immunohistochemical approaches carried out on sections from prenatal human testes. Thus, the present study identifies the Leydig cells as the presumed sites of catecholamine production in both the mature and fetal human testes and further supports the previously recognized neuroendocrine characteristics of this cell type.
Davidoff
Michail S
MS
Institute of Anatomy, University Hospital Hamburg, Martinistrasse 52, 20246 Hamburg, Germany. davidoff@uke.uni-hamburg.de
Ungefroren
Hendrik
H
Middendorff
Ralf
R
Koeva
Yvetta
Y
Bakalska
Mariana
M
Atanassova
Nina
N
Holstein
Adolf F
AF
Jezek
Davor
D
Pusch
Wolfgang
W
Müller
Dieter
D
D000368:Aged; D000369:Aged, 80 and over; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism*; D002395:Catecholamines / Q000096:biosynthesis*; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007985:Leydig Cells / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D010625:Phenylethanolamine N-Methyltransferase / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000196:embryology / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D002395:Catecholamines; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D010625:Phenylethanolamine N-Methyltransferase; D001142:Aromatic-L-Amino-Acid Decarboxylases