title: Noncovalent modification of chymotrypsin surface using an amphiphilic polymer scaffold: implications in modulating protein function.
abstract: We report here on a new amphiphilic homopolymer that binds noncovalently to proteins. This polymer not only binds to the target protein chymotrypsin with submicromolar affinity but also stabilizes the native structure of the protein. Since the polymer-protein binding process is based on electrostatic interaction, the bound protein can be released from the polymer surface and reactivated either by increasing the ionic strength or by adding complementary cationic surfactants. The electrostatic binding of polymer to the protein results in a marked change in the substrate specificity of chymotrypsin.
authors.affiliation: Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors.forename: Britto S
authors.initials: BS
authors.lastname: Sandanaraj
authors.forename: Dharma Rao
authors.initials: DR
authors.lastname: Vutukuri
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Simard
authors.forename: Akamol
authors.initials: A
authors.lastname: Klaikherd
authors.forename: Rui
authors.initials: R
authors.lastname: Hong
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
authors.forename: S
authors.initials: S
authors.lastname: Thayumanavan
mesh_terms: D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008823:Micelles; D008968:Molecular Conformation; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity; D013499:Surface Properties
chemical_list: D008823:Micelles; D011108:Polymers; D010649:Phenylalanine; C018389:N-succinylglycyl-phenylalanine-4-nitroanilide; D002918:Chymotrypsin