title: Interactions between CAP70 and actinfilin are important for integrity of actin cytoskeleton structures in neurons.
abstract: The integrity of dynamic actin structures is coupled to a variety of neurological processes. Actin-binding proteins play a critical role in regulating actin structure dynamics. A link between actin-binding proteins and receptor interacting scaffolding proteins may provide a conduit for transmitting signaling events to the cytoskeleton. Actinfilin is a brain-enriched actin-binding protein, though its functions are currently unknown. We report here that actinfilin interacts with the multi-PDZ domain protein CAP70. Recombinant expression of an actin-binding domain of actinfilin progressively causes marked changes of cellular morphology. The effect on cell morphology may be reduced by co-expression with CAP70. Mutation of actinfilin lacking the ability to interact with CAP70 abolished the effect by CAP70. The evidence suggests a role of actinfilin and possible regulation by scaffolding proteins.
authors.affiliation: Department of Neuroscience and High Throughput Biology Center, Johns Hopkins University School of Medicine, BRB311, Baltimore, MD 21205, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Li
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000648:ultrastructure; D002352:Carrier Proteins / Q000737:chemistry / Q000502:physiology*; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000737:chemistry / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D017403:In Situ Hybridization; D009474:Neurons / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012441:Saccharomyces cerevisiae; D013347:Subcellular Fractions / Q000187:drug effects / Q000648:ultrastructure; D014162:Transfection
chemical_list: D000199:Actins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C109245:Pdzk1 protein, rat