Inhibition of septic arthritis by local administration of taurine chloramine, a product of activated neutrophils.
OBJECTIVE
Taurine is an amino acid able to react with hypochlorous acid, produced endogenously by neutrophils, resulting in the more stable and less toxic taurine chloramine (Tau-Cl). Since Tau-Cl has been shown to down-regulate the production of proinflammatory mediators and to exert anti-bacterial properties, we investigated the efficacy of Tau-Cl treatment for infectious arthritis.


METHODS
The murine model of hematogenous septic arthritis involved intravenous injection of a single dose of Staphylococcus aureus. Tau-Cl was administered by daily intraperitoneal injections. In another experiment S. aureus and Tau-Cl were injected intra-articularly. Evaluation of arthritis was performed clinically and histologically. The effect of Tau-Cl on bacterial growth in vitro was also assessed.


RESULTS
Growth of staphylococci, including the methicillin-resistant strain 67-0, was inhibited by Tau-Cl. Mice injected with bacteria and Tau-Cl locally in the joint exhibited significantly fewer arthritic lesions. In contrast, there were no obvious differences between Tau-Cl-treated animals and controls with regard to clinical or histological signs of arthritis when bacteria and Tau-Cl were administered systemically.


CONCLUSIONS
Our results show that Tau-Cl exerts an inhibitory effect on the development of bone and cartilage damage in the infected joint when administered intra-articularly.
Verdrengh
Margareta
M
Department of Rheumatology and Inflammation Research, Göteborg University, Sweden. margareta.verdrengh@rheuma.gu.se
Tarkowski
Andrej
A
D000818:Animals; D001170:Arthritis, Infectious / Q000188:drug therapy* / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects / Q000276:immunology; D005260:Female; D018836:Inflammation Mediators / Q000494:pharmacology*; D007270:Injections, Intra-Articular; D015850:Interleukin-6 / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D016106:Methicillin Resistance; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000276:immunology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology
D018836:Inflammation Mediators; D015850:Interleukin-6; D013654:Taurine; C043410:N-chlorotaurine