title: Rabbit monoclonal antibodies: a comparative study between a novel category of immunoreagents and the corresponding mouse monoclonal antibodies.
abstract: Rabbit monoclonal antibodies (RabMAbs) represent a novel category of immunoreagents that may combine the best properties of both mouse monoclonal antibodies (MMAs) and of rabbit antisera. In the attempt to verify the performance of this new class of antibodies on paraffin-embedded tissue, RabMAbs against estrogen receptor, progesterone receptor, Ki-67, cyclin D1, CD3, CD5, CD23, and synaptophysin were tested on several tumor types as well as normal tissues. The results were compared with those obtained with classic MMAs against the same antigens. RabMAbs appear to offer increased sensitivity with no apparent loss of specificity. On routine use they permit higher working dilutions (5 to 10 times on average), allowing significant improvement in terms of laboratory efficiency. The robustness of RabMAbs is further proved by the fact that in some instances optimal staining can be obtained even without antigen retrieval. In consideration of the high performance observed, routine use of RabMAbs may contribute significantly to standardize diagnostic immunohistochemical procedures.
authors.affiliation: Department of Pathology, Regional Hospital, Treviso, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Rossi
authors.forename: Licia
authors.initials: L
authors.lastname: Laurino
authors.forename: Alberto
authors.initials: A
authors.lastname: Furlanetto
authors.forename: Serena
authors.initials: S
authors.lastname: Chinellato
authors.forename: Enrico
authors.initials: E
authors.lastname: Orvieto
authors.forename: Fabio
authors.initials: F
authors.lastname: Canal
authors.forename: Fabio
authors.initials: F
authors.lastname: Facchetti
authors.forename: Angelo P
authors.initials: AP
authors.lastname: Dei Tos
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis*; D017252:CD3 Complex / Q000378:metabolism; D018956:CD5 Antigens / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods*; D019394:Ki-67 Antigen / Q000378:metabolism; D051379:Mice; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D011817:Rabbits; D011960:Receptors, Estrogen / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D012680:Sensitivity and Specificity; D016708:Synaptophysin / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D017252:CD3 Complex; D018956:CD5 Antigens; D019394:Ki-67 Antigen; D011960:Receptors, Estrogen; D017455:Receptors, IgE; D011980:Receptors, Progesterone; D016708:Synaptophysin; D019938:Cyclin D1