title: Peripheral T cells of patients with B cell non-Hodgkin's lymphoma show a shift in their memory status.
abstract: BACKGROUND
  Tumor-infiltrating T cells have a positive influence on the clinical course of B cell non-Hodgkin's lymphoma (NHL). T cells in the peripheral blood of patients with B cell NHL, however, have so far rarely been examined.
  
  
  METHODS
  Using flow cytometry we examined lymphocyte subpopulations and numbers of naïve/memory T cell subtypes among peripheral T cells of patients with B cell NHL (N=22), patients with metastasized solid tumors (N=27), and healthy controls (N=20). In addition, we analyzed the intracellular content of effector molecules granzyme B and perforin and expression of the T cell receptor zeta chain.
  
  
  RESULTS
  We observed increased percentages of potentially highly cytotoxic CD8+CD56+ T cells in the peripheral blood of patients with NHL. Both, patients with NHL and patients with solid tumors showed a much higher expression of the chemokine receptors CCR4 and CCR5 on their T cells than healthy controls, suggesting a polarization of their T cells following stimulation with antigen and/or cytokines in vivo. Furthermore, patients with B cell NHL and patients with solid tumors had far lower percentages of naïve CD45RA+CCR7+ T cells than healthy controls and, in the case of CD4+ T cells, patients with solid tumors. In contrast, patients with B cell NHL showed markedly increased levels of memory effector CD45RA-CCR7- CD4(+) T cells when compared to healthy controls and patients with metastasized solid tumors. Patients with NHL also showed elevated levels granzyme B within CD8(+) T cells, indicating that the increase in memory effector cells was of functional relevance.
  
  
  CONCLUSIONS
  These findings indicate a marked shift in the composition of peripheral T cells of patients with B cell NHL from naïve to memory effector-type cells.
authors.affiliation: Department of Medicine, Oncology/Hematology, University Hospital Eppendorf, Martinistr. 52, 20246 Hamburg, Germany.
authors.forename: Djordje
authors.initials: D
authors.lastname: Atanackovic
authors.forename: Jens
authors.initials: J
authors.lastname: Panse
authors.forename: Philippe
authors.initials: P
authors.lastname: Schafhausen
authors.forename: Christiane
authors.initials: C
authors.lastname: Faltz
authors.forename: Katrin
authors.initials: K
authors.lastname: Bartels
authors.forename: Ina
authors.initials: I
authors.lastname: Boeters
authors.forename: Dieter Kurt
authors.initials: DK
authors.lastname: Hossfeld
authors.forename: Susanna
authors.initials: S
authors.lastname: Hegewisch-Becker
mesh_terms: D016022:Case-Control Studies; D006801:Humans; D007156:Immunologic Memory; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D016393:Lymphoma, B-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*