title: Identification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico.
abstract: Three isolates of Histoplasma capsulatum were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel's ornamental potted plants that had been fertilized with organic material known as compost. The presence of H. capsulatum in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual histoplasmosis outbreak in Acapulco. Although, diversity between the H. capsulatum isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of H-anti and ole fragment genes revealed a high homology (92-99%) between them.
authors.affiliation: Laboratorio de Inmunología de Hongos, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma México (UNAM), Ciudad Universitaria s/n. emello@servidor.unam.mx
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Taylor
authors.forename: Guillermo M
authors.initials: GM
authors.lastname: Ruíz-Palacios
authors.forename: María
authors.initials: M
authors.lastname: del Rocío Reyes-Montes
authors.forename: Gabriela
authors.initials: G
authors.lastname: Rodríguez-Arellanes
authors.forename: Laura E
authors.initials: LE
authors.lastname: Carreto-Binaghi
authors.forename: Esperanza
authors.initials: E
authors.lastname: Duarte-Escalante
authors.forename: Aurora
authors.initials: A
authors.lastname: Hernández-Ramírez
authors.forename: Armando
authors.initials: A
authors.lastname: Pérez
authors.forename: Roberto O
authors.initials: RO
authors.lastname: Suárez-Alvarez
authors.forename: Yuri A
authors.initials: YA
authors.lastname: Roldán-Aragón
authors.forename: Rafael
authors.initials: R
authors.lastname: Romero-Martínez
authors.forename: Jorge H
authors.initials: JH
authors.lastname: Sahaza-Cardona
authors.forename: José
authors.initials: J
authors.lastname: Sifuentes-Osornio
authors.forename: Luis E
authors.initials: LE
authors.lastname: Soto-Ramírez
authors.forename: Gabriela R
authors.initials: GR
authors.lastname: Peña-Sandoval
mesh_terms: D000818:Animals; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005656:Fungal Proteins / Q000235:genetics; D006658:Histoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006660:Histoplasmosis / Q000453:epidemiology* / Q000382:microbiology / Q000473:pathology; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014195:Travel
chemical_list: D005656:Fungal Proteins