title: Decline in fertility of mouse sperm with abnormal chromatin during epididymal passage as revealed by ICSI.
abstract: BACKGROUND
  Recent studies showed that ICSI with cauda epididymal or ejaculated sperm of infertile mice or men, respectively, was less effective in fertilization and normal embryo development than ICSI using sperm from the testes. These studies suggested that sperm nuclear quality declined after release from the testis, but the site where this loss of fertility occurs has not been localized.
  
  
  METHODS
  We performed ICSI with testicular, caput, and cauda epididymal sperm from infertile Tnp1-/-Tnp2+/- mutant mice, which have a minimal level of transition nuclear proteins and are sterile by natural mating.
  
  
  RESULTS
  When the heads of motile sperm from the testis or caput epididymis of Tnp1-/-Tnp2+/- males were injected into enucleated mouse oocytes, sperm chromosomes showed no difference from those of wild-type mice, but the chromosomes from sperm taken from the cauda epididymis of mutant males showed increased abnormalities. Injection of testicular or caput epididymal sperm from Tnp1-/-Tnp2+/- males into intact oocytes resulted in normal embryonic and fetal development and yields of liveborn equivalent to wild-type, but cauda sperm from Tnp1-/-Tnp2-/- mice produced lower implantation rates and yields of liveborn than did those from wild-type mice.
  
  
  CONCLUSIONS
  These results demonstrate that in mice with sperm chromatin abnormalities, the decline in fertility of sperm with ICSI occurs after the caput epididymis. The advantage of using caput epididymal sperm for ICSI in certain situations may be considered as an approach to be tested in human assisted reproduction.
authors.affiliation: Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu, HI 96822, USA.
authors.forename: Ryota
authors.initials: R
authors.lastname: Suganuma
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Yanagimachi
authors.forename: Marvin L
authors.initials: ML
authors.lastname: Meistrich
mesh_terms: D000818:Animals; D002843:Chromatin / Q000737:chemistry*; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology; D004268:DNA-Binding Proteins; D004822:Epididymis / Q000166:cytology*; D005260:Female; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009687:Nuclear Proteins / Q000502:physiology; D011479:Protamines / Q000378:metabolism; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D011479:Protamines; C489505:Tnp2 protein, mouse; C053886:spermatid transition proteins