Isocratic high-performance liquid chromatographic method with ultraviolet detection for simultaneous determination of levels of voriconazole and itraconazole and its hydroxy metabolite in human serum.
A simple, specific method is presented for simultaneous determination of voriconazole and itraconazole and its metabolite, hydroxyitraconazole, in human serum using one-step liquid-liquid extraction and high-performance liquid chromatography. Linearity tests ranged from 0.1 to 8.0 microg/ml; the minimum detectable concentration was 0.03 microg/ml.
Khoschsorur
GholamAli
G
Institute of Medical and Chemical Laboratory Diagnostics (LB.I), Auenbruggerplatz 29, A-8036 Graz, Austria. g.Khoschsorur@klinikum-graz.at
Fruehwirth
Franz
F
Zelzer
Sieglinde
S
D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D017964:Itraconazole / Q000031:analogs & derivatives* / Q000097:blood*; D011743:Pyrimidines / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014230:Triazoles / Q000097:blood*; D014466:Ultraviolet Rays; D065819:Voriconazole
D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; C084794:hydroxyitraconazole; D017964:Itraconazole; D065819:Voriconazole