Identification and molecular cloning of a gene encoding Phospholipase A2 (plaA) from Aspergillus nidulans.
The plaA gene encoding a protein that contains the cytosolic Phospholipase A(2) (cPLA(2)) motif is cloned for the first time from the filamentous fungus, Aspergillus nidulans. The translated 837 amino acid protein product of plaA comprises conserved lipase regions that are present in most mammalian cPLA(2) homologs. High expression of plaA was observed in glucose-lactose medium by Northern blot analyses. Deletion mutants of plaA grew and formed conidia similar to the wild-type strain, but showed decreased PLA(2) activity. Expression of the N-terminal truncated form of plaA in yeast cells resulted in increased Ca(2+)-dependent PLA(2) activity with (14)C-labeled phosphatidylcholine (PC) and phosphatidylethanolamine (PE) as substrates, compared with vector-transformed cells. In conclusion, we have identified and cloned a phospholipid-hydrolyzing novel cPLA(2) protein from A. nidulans for the first time.
Hong
SaHyun
S
Department of Biotechnology, The University of Tokyo, Japan. strepto13@hotmail.com
Horiuchi
Hiroyuki
H
Ohta
Akinori
A
D000595:Amino Acid Sequence; D000818:Animals; D001233:Aspergillus nidulans / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D005656:Fungal Proteins / Q000235:genetics; D005784:Gene Amplification; D006801:Humans; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D010802:Phylogeny; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D005656:Fungal Proteins; D011994:Recombinant Proteins; D010741:Phospholipases A; D054467:Phospholipases A2