Apoptosis of pancreatic cancer BXPC-3 cells induced by indole-3-acetic acid in combination with horseradish peroxidase.
OBJECTIVE
To explore the mechanisms underlying the apoptosis of human pancreatic cancer BXPC-3 cells induced by indole-3-acetic acid (IAA) in combination with horseradish peroxidase (HRP).


METHODS
BXPC-3 cells derived from human pancreatic cancer were exposed to 40 or 80 micromol/L IAA and 1.2 microg/mL HRP at different times. Then, MTT assay was used to detect the cell proliferation. Flow cytometry was performed to analyze cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay was used to detect apoptosis. 2,7-Dichlorofluorescin diacetate uptake was measured by confocal microscopy to determine free radicals. Level of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were measured by biochemical methods.


RESULTS
IAA/HRP initiated growth inhibition of BXPC-3 cells in a dose- and time-dependent manner. Flow cytometry revealed that the cells treated for 48 h were arrested at G1/G0. After exposure to 80 micromol/L IAA plus 1.2 microg/mL HRP for 72 h, the apoptosis rate increased to 72.5 per thousand, which was nine times that of control. Content of MDA and activity of SOD increased respectively after treatment compared to control. Meanwhile, IAA/HRP stimulated the formation of free radicals.


CONCLUSIONS
The combination of IAA and HRP can inhibit the growth of human pancreatic cancer BXPC-3 cells in vitro by inducing apoptosis.
Huang
Chen
C
Department of Cytobiology and Medical Genetics, College of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
Liu
Li-Ying
LY
Song
Tu-Sheng
TS
Ni
Lei
L
Yang
Ling
L
Hu
Xiao-Yan
XY
Hu
Jing-Song
JS
Song
Li-Ping
LP
Luo
Yu
Y
Si
Lu-Sheng
LS
D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004359:Drug Therapy, Combination; D006735:Horseradish Peroxidase / Q000494:pharmacology*; D006801:Humans; D007210:Indoleacetic Acids / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000188:drug therapy*; D010937:Plant Growth Regulators / Q000494:pharmacology*
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D006735:Horseradish Peroxidase