title: Nonradioactive telomerase activity assay by microchip electrophoresis: privileges to the classical gel electrophoresis assay.
abstract: The present study accents on the privileges of microchip-based electrophoresis to the conventional gel electrophoresis in separation of telomerase repeat amplification protocol/polymerase chain reaction (PCR) ladder products obtained in telomerase-catalyzed reaction in cancer cells. We try to clarify the interpretation of the results obtained by both electrophoretic procedures and to avoid misinterpretation as a result of PCR-dependent artefacts.
authors.affiliation: Single-Molecule Bioanalysis Laboratory (SMBL), National Institute for Advanced Industrial Science and Technology, AIST-Shikoku, Takamatsu, Japan.
authors.forename: Zhivko
authors.initials: Z
authors.lastname: Zhelev
authors.forename: Rumiana
authors.initials: R
authors.lastname: Bakalova
authors.forename: Ashraf
authors.initials: A
authors.lastname: Ewis
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohba
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D046209:Electrophoresis, Microchip; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D020014:K562 Cells; D016133:Polymerase Chain Reaction; D019098:Telomerase / Q000032:analysis / Q000378:metabolism*
chemical_list: D019098:Telomerase