title: Dogs cloned from adult somatic cells.
abstract: Several mammals--including sheep, mice, cows, goats, pigs, rabbits, cats, a mule, a horse and a litter of three rats--have been cloned by transfer of a nucleus from a somatic cell into an egg cell (oocyte) that has had its nucleus removed. This technology has not so far been successful in dogs because of the difficulty of maturing canine oocytes in vitro. Here we describe the cloning of two Afghan hounds by nuclear transfer from adult skin cells into oocytes that had matured in vivo. Together with detailed sequence information generated by the canine-genome project, the ability to clone dogs by somatic-cell nuclear transfer should help to determine genetic and environmental contributions to the diverse biological and behavioural traits associated with the many different canine breeds.
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Gwanak-gu, Seoul 151-742, South Korea.
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
authors.forename: Min Kyu
authors.initials: MK
authors.lastname: Kim
authors.forename: Goo
authors.initials: G
authors.lastname: Jang
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Oh
authors.forename: Fibrianto
authors.initials: F
authors.lastname: Yuda
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: M Shamim
authors.initials: MS
authors.lastname: Hossein
authors.forename: M Hossein
authors.initials: MH
authors.lastname: Shamim
authors.forename: Jung Ju
authors.initials: JJ
authors.lastname: Kim
authors.forename: Sung Keun
authors.initials: SK
authors.lastname: Kang
authors.forename: Gerald
authors.initials: G
authors.lastname: Schatten
authors.forename: Woo Suk
authors.initials: WS
authors.lastname: Hwang
mesh_terms: D000375:Aging; D000818:Animals; D019976:Cloning, Organism; D004285:Dogs / Q000145:classification / Q000196:embryology / Q000235:genetics* / Q000254:growth & development*; D004624:Embryo Transfer; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D053652:Nuclear Transfer Techniques; D018587:Oocyte Donation; D009865:Oocytes / Q000166:cytology / Q000502:physiology / Q000637:transplantation; D011247:Pregnancy; D012867:Skin / Q000166:cytology; D013997:Time Factors