title: Alteration of gonadotropin-releasing hormone receptor expression with the progression of prostate cancer in the Dunning rat adenocarcinoma sublines.
abstract: Inhibitory effects of GnRH analogues on tumour growth in vitro suggests that such direct effects may be of importance also in vivo. However, the role of GnRH receptors (GnRH-R) in prostate tumour progression is largely unknown. The aim was therefore to investigate the variation of GnRH-R expression with prostate tumour progression using Dunning rat adenocarcinoma sublines representing different prostate tumour grades. GnRH-R levels were quantified in the rat dorsolateral (DLP) and Dunning sublines (PAP, AT-1, AT-2, AT-3, MatLyLu) using competitive RT-PCR and Western blot. The results showed that all Dunning sublines had significantly elevated GnRH-R mRNA expression levels compared with DLP. Comparison of GnRH-R mRNA levels between different tumour grades revealed no difference in mRNA expression. However, the anaplastic and highly metastatic AT-3 and MatLyLu tumours displayed a tendency for lower GnRH-R mRNA values than the non-metastatic tumour sublines. Our data demonstrate the expression of GnRH-R in normal rat DLP and in different Dunning sublines. However, GnRH-R seems not to be involved in tumour progression.
authors.affiliation: Institute of Surgical and Perioperative Sciences, Urology & Andrology, Umeå University, Umeå, Sweden. ase.tieva@onkologi.umu.se
authors.forename: Ase
authors.initials: A
authors.lastname: Tieva
authors.forename: Anders
authors.initials: A
authors.lastname: Bergh
authors.forename: Jan-Erik
authors.initials: JE
authors.lastname: Damber
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology* / Q000556:secondary; D000708:Anaplasia; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D008297:Male; D011467:Prostate / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011966:Receptors, LHRH / Q000032:analysis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D011966:Receptors, LHRH