[Differential gene expression in nasopharyngeal carcinoma cell with reduced and normal expression of 6A8 alpha-mannosidase].
OBJECTIVE
To detect the differential display of mRNA expression between human nasopharyngeal carcinoma cell CNE-2L2 with reduced malignancy caused by transduction of a DNA antisense to 6A8 alpha-mannosidase cDNA (AS cell) and the wild type cell (W cell).


METHODS
Differential display of mRNA expression was analyzed using DNA microarray analysis. The datasets were confirmed by Northern blotting and RT-PCR.


RESULTS
Out of the 1069 genes analyzed, 34 genes were up-regulated in AS cells relative to W cells. Conversely, 42 genes were down-regulated. The genes, up-regulation of which might have suppressive effect on tumor malignant behaviors, were P130 mRNA for 130K protein, TGF-betaIIR alpha, GABBR1, TGFBR1, TNFAIP1, STANIN, E-CADHERIN, CTNNA1 and 2, RFX2, TMPO, etc. The genes, down-regulation of which might have suppressive effect on tumor malignant behaviors, were CD44, NDRG1, TGFB1, RPS5, LEGUMAIIN, CBS, CD59, SNRPA1, etc. The microarray datasets were confirmed by Northern blot and RT-PCR analysis.


CONCLUSIONS
In comparison to the W cell, AS cell has up-regulation of 34 genes and down-regulation of 42 genes. Changes of the gene expression may play a role in the malignancy reduction of AS cell.
Chen
Shuang-ling
SL
Department of Immunology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Shi
Yan
Y
Jin
Yu-lan
YL
Liu
Yin
Y
Zhao
Fang-tao
FT
Zhu
Li-ping
LP
D020869:Gene Expression Profiling; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000201:enzymology* / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D014407:Tumor Cells, Cultured; D043323:alpha-Mannosidase / Q000096:biosynthesis* / Q000235:genetics
D043323:alpha-Mannosidase