LT, VP1 and TCR-BKV sequence analysis in a patient with post-transplant BKV nephropathy associated with EBV-related PTLD.
A 10-year-old boy kidney transplant recipient (KTR) developed an abdominal post-transplant lymphoproliferative syndrome (PTLD) followed by BK virus nephropathy (BKVN). BK virus (BKV) and Epstein-Barr virus (EBV) were studied in renal and PTLD tissue by polymerase chain reaction (PCR) assay. Afterwards, the patient was monitored in relation to BKV in urine and serum; transcription control region (TCR)-BK polymorphism sequence analysis was also performed. In the PCR assay, both early large T antigen (LT) and late (VP1) transcriptional BKV coding regions were found in renal tissue, whereas EBV and only LT-BK were detected in PTLD abdominal tissue. On the other hand, TCR sequence analysis revealed the AS genomic BK variant.
Rubio
Luis
L
Department of Pathology, Laboratory of Molecular Pathology, University Hospital La Fe, Medical School of Valencia University, Avda. Campanar 21, 46009 Valencia, Spain.
Vera-Sempere
Francisco J
FJ
Moreno-Baylach
María J
MJ
García
Ana
A
Zamora
Isabel
I
Simón
José
J
D000957:Antigens, Viral, Tumor / Q000235:genetics; D001739:BK Virus / Q000235:genetics*; D002648:Child; D020031:Epstein-Barr Virus Infections / Q000150:complications*; D006801:Humans; D007674:Kidney Diseases / Q000821:virology*; D016030:Kidney Transplantation / Q000009:adverse effects*; D008232:Lymphoproliferative Disorders / Q000209:etiology*; D008297:Male; D027601:Polyomavirus Infections / Q000150:complications*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D017421:Sequence Analysis; D014158:Transcription, Genetic / Q000235:genetics; D014412:Tumor Virus Infections / Q000150:complications*
D000957:Antigens, Viral, Tumor