Altered protein expression in endometrial carcinogenesis.
We have discovered several protein biomarkers that are altered during carcinogenesis of the human uterine endometrium. Proteins prepared from 19 endometrial carcinomas (Group A), and 20 normal endometria obtained from benign gynecological conditions (Group B), were investigated by Surface Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF-MS). Two proteins, EC1 and EC2, were consistently expressed differentially. EC1 had an increased level of expression in carcinomas (P<0.001), while EC2 was expressed at a lower level (P=0.004). The isoelectric points of EC1 and EC2 were approximately pH 5.0 and 7.0, respectively. These proteins are thus potential biomarkers for detection of endometrial carcinoma.
Yoshizaki
Tatsuo
T
Department of Obstetrics and Gynecology, Faculty of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
Enomoto
Takayuki
T
Nakashima
Ryuichi
R
Ueda
Yutaka
Y
Kanao
Hiroyuki
H
Yoshino
Kiyoshi
K
Fukumoto
Masahiro
M
Yoneda
Yoshihiro
Y
Buzard
Gregory S
GS
Murata
Yuji
Y
D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008875:Middle Aged; D040901:Proteomics
D014408:Biomarkers, Tumor