Amburoside A, a glucoside from Amburanacearensis, protects mesencephalic cells against 6-hydroxydopamine-induced neurotoxicity.
This study evaluates the potential neuroprotective properties of amburoside A, a glucoside isolated from Amburana cearensis, on rat mesencephalic cell cultures exposure to the neurotoxin 6-hydroxydopamine (6-OHDA). The parameters determined were cell viability by the 3[4,5-dimethylthiazole-2-il]-2,5-diphenyltetrazolium bromide (MTT) method, nitric oxide (NO) and free radical formation by the measurement of nitrite concentration and thiobarbituric acid reacting substance (TBARS) formation as an indication of cellular lipid peroxidation. The results showed that AMB was less effective as a curative agent in the MTT assay, since its addition after 6-OHDA did not reverse the neurotoxin's effect, except at the highest concentration (AMB, 100 microg/ml). Similarly, the higher nitrite levels observed after exposure of the cells to 6-OHDA were only partially reversed by AMB, at this highest concentration. However, when AMB (0.5, 1, 10 and 100 microg/ml) was added before the toxin, it appeared to protect neuronal cells against 6-OHDA toxicity in a concentration-dependent manner, as shown by MTT assay. AMB also prevented free radical formation indicated by the increased nitrite concentration induced by 6-OHDA. Cells exposed to 6-OHDA showed a 3.4 times increase in TBARS concentration as compared to controls, and this effect was inhibited from 24% up to 64% by AMB (0.1-100 microg/ml), indicative of a neuroprotective effect. In conclusion, we show that AMB, acting as an antioxidant compound, presents a significant neuroprotective effect, suggesting that this compound could provide benefits as a therapeutic agent in neurodegenerative disease such as Parkinson's.
Leal
L K A M
LK
Department of Pharmacy, Federal University of Ceará, 60430-270 Fortaleza, Ceará, Brazil. kalyne@ufc.br
Nobre Júnior
H V
HV
Cunha
G M A
GM
Moraes
M O
MO
Pessoa
C
C
Oliveira
R A
RA
Silveira
E R
ER
Canuto
K M
KM
Viana
G S B
GS
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D004347:Drug Interactions; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D066298:In Vitro Techniques; D008636:Mesencephalon / Q000166:cytology; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D009498:Neurotoxins / Q000494:pharmacology; D016627:Oxidopamine / Q000494:pharmacology; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013565:Sympatholytics / Q000494:pharmacology
D000975:Antioxidants; D005960:Glucosides; D018696:Neuroprotective Agents; D009498:Neurotoxins; D010936:Plant Extracts; D013565:Sympatholytics; D016627:Oxidopamine