Regular expression of discoidin domain receptor 2 in the improved adjuvant-induced animal model for rheumatoid arthritis.
OBJECTIVE
To investigate the expression of discoidin domain receptor 2 (DDR2) of fibroblast-like synovial cells in improved adjuvant-induced animal (AIA) model for rheumatoid arthritis (RA) and to provide evidence for DDR2's antagonist use clinically.


METHODS
AIA was modified by administrating 0.1 mL of complete Freund's adjuvant (CFA, mixed with 5 mg Bacillus Calmette-Guerin vaccine/mL) into rats' right hind paws and 0.125 mL tumor necrosis factor-alpha (2 U/mL) into right ankles and subpatellar fatty tissue. The expression of DDR2 in fibroblast-like synovial cells was assessed using immunohistochemistry, immunofluorescence histochemistry, and in situ hybridization methods. Levels of anti-collagen II antibody were measured using enzyme-linked immunosorbent assay.


RESULTS
Given the terms mentioned above, we found a more practical rat model, apparently decreasing immunization time (average 3-5 days). DDR2 can be detected upon the 15th day of immunization; expression gradually increased with time going on, and reaching a peak 35 days after immunization before gradually decreasing. Serum anti-collagen II antibody showed similar expression patterns as DDR2, but reached peak later than DDR2, about 40 days after immunization.


CONCLUSIONS
Regular expression of DDR2 in animal models infers its important role in the pathological process of RA.
Li
Wei
W
Department of Histology and Embryology, the Fourth Military University, Xi'an.
Zhang
Yuan-qiang
YQ
Liu
Xin-ping
XP
Yao
Li-bo
LB
Sun
Lan
L
D000818:Animals; D000906:Antibodies / Q000097:blood; D001169:Arthritis, Experimental / Q000209:etiology / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000139:chemically induced / Q000378:metabolism*; D001500:BCG Vaccine; D024043:Collagen Type II / Q000276:immunology; D000070818:Discoidin Domain Receptors; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005620:Freund's Adjuvant; D051381:Rats; D017207:Rats, Sprague-Dawley; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D011975:Receptors, Mitogen / Q000378:metabolism*; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism*
D000906:Antibodies; D001500:BCG Vaccine; D024043:Collagen Type II; D011975:Receptors, Mitogen; D005620:Freund's Adjuvant; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases