Ligand-regulated peptides: a general approach for modulating protein-peptide interactions with small molecules.
We engineered a novel ligand-regulated peptide (LiRP) system where the binding activity of intracellular peptides is controlled by a cell-permeable small molecule. In the absence of ligand, peptides expressed as fusions in an FKBP-peptide-FRB-GST LiRP scaffold protein are free to interact with target proteins. In the presence of the ligand rapamycin, or the nonimmunosuppressive rapamycin derivative AP23102, the scaffold protein undergoes a conformational change that prevents the interaction of the peptide with the target protein. The modular design of the scaffold enables the creation of LiRPs through rational design or selection from combinatorial peptide libraries. Using these methods, we identified LiRPs that interact with three independent targets: retinoblastoma protein, c-Src, and the AMP-activated protein kinase. The LiRP system should provide a general method to temporally and spatially regulate protein function in cells and organisms.
Binkowski
Brock F
BF
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Miller
Russell A
RA
Belshaw
Peter J
PJ
D055372:AMP-Activated Protein Kinases; D000595:Amino Acid Sequence; D001665:Binding Sites; D002478:Cells, Cultured; D004347:Drug Interactions; D005982:Glutathione Transferase / Q000378:metabolism*; D008024:Ligands; D009097:Multienzyme Complexes / Q000378:metabolism; D019151:Peptide Library; D010455:Peptides / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000235:genetics / Q000378:metabolism; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism*; D022021:Tacrolimus Binding Proteins / Q000235:genetics / Q000378:metabolism*
D008024:Ligands; D009097:Multienzyme Complexes; D019151:Peptide Library; D010455:Peptides; D016160:Retinoblastoma Protein; D005982:Glutathione Transferase; D016392:Proto-Oncogene Proteins pp60(c-src); D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases; D022021:Tacrolimus Binding Proteins