Inducement and reversal of tetracycline resistance in Escherichia coli K-12 and expression of proton gradient-dependent multidrug efflux pump genes.
Expression of eight transporter genes of Escherichia coli K-12 and its DeltaacrAB mutant prior to and after induction of both strains to tetracycline resistance and after reversal of induced resistance were analyzed by quantitative reverse transcriptase PCR. All transporter genes were overexpressed after induced resistance with acrF being 80-fold more expressed in the DeltaacrAB tetracycline-induced strain.
Viveiros
Miguel
M
Unit of Mycobacteriology, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua Junqueira 96, 1349-008 Lisbon, Portugal.
Jesus
Ana
A
Brito
Mafalda
M
Leandro
Clara
C
Martins
Marta
M
Ordway
Diane
D
Molnar
Ana Maria
AM
Molnar
Joseph
J
Amaral
Leonard
L
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D048168:Escherichia coli K12 / Q000187:drug effects*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008826:Microbial Sensitivity Tests; D009154:Mutation; D017494:Proton Pumps / Q000378:metabolism*; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance
D000900:Anti-Bacterial Agents; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D017494:Proton Pumps; D013752:Tetracycline