Antioxidant activity of Glossogyne tenuifolia.
Glossogyne tenuifolia is a native traditional anti-inflammatory herb in Taiwan. It has previously been shown that the ethanol extract of G. tenuifolia (GT) inhibited the LPS-induced inflammatory mediator release from murine macrophage cell line and human whole blood. In the present work, the ethanol extracts of G. tenuifolia and its major constituent, luteolin-7-glucoside, were shown to be scavengers of 1,1-diphenyl-2-picrylhydrazyl, superoxide, and hydroxyl radicals. Moreover, copper-induced low-density lipoprotein oxidation was suppressed by GT and luteolin-7-glucoside as measured by decreased formation of malondialdehyde and conjugated diene as well as reduced electrophoretic mobility. GT and luteolin-7-glucoside were also against N-formyl-methionyl-leucyl-phenylalanine-induced reactive oxygen species (ROS) production in human polymorphonuclear neutrophils and peripheral blood mononuclear cells. In summary, these data indicated that GT is a potential ROS scavenger and may prevent atherosclerosis via inhibiting LDL oxidation or ROS production in human leukocytes. Moreover, luteolin-7-glucoside may serve as the active principal of GT.
Wu
Ming-Jiuan
MJ
Department of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan 717, Taiwan. imwu@mail.chna.edu.tw
Huang
Chia-Lin
CL
Lian
Tzi-Wei
TW
Kou
Mei-Chuan
MC
Wang
Lisu
L
D000975:Antioxidants / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D001713:Biphenyl Compounds; D000431:Ethanol; D016166:Free Radical Scavengers; D005960:Glucosides / Q000494:pharmacology; D006801:Humans; D017665:Hydroxyl Radical; D007963:Leukocytes, Mononuclear / Q000502:physiology; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000737:chemistry; D047311:Luteolin / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects; D013481:Superoxides
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005960:Glucosides; D008077:Lipoproteins, LDL; D010851:Picrates; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013481:Superoxides; D017665:Hydroxyl Radical; D000431:Ethanol; D009240:N-Formylmethionine Leucyl-Phenylalanine; C066408:luteolin-7-glucoside; C004931:1,1-diphenyl-2-picrylhydrazyl; D047311:Luteolin