Mouse thymocyte apoptosis and cell loss in response to exercise and antioxidant administration.
Various physical and psychological stressors can cause thymocyte apoptosis and cell loss in rodents. Although glucocorticoids (GC) are commonly implicated, oxidative stress may also play a role. The purpose of this study was to examine the effect of an acute bout of strenuous treadmill running, and the antioxidant N-acetyl-L-cysteine (NAC) on thymocyte loss and apoptosis. Eighty-eight female C57BL/6 mice were given NAC (1 g/kg, i.p.) or saline (SAL) 30 min before 90 min of treadmill exercise at a 2 degrees slope (EX; 30 min at 22 m/min; 30 min at 25 m/min; and 30 min at 28 m/min) and sacrificed immediately (Imm) or 24 h following EX. Control mice (NonEX) were exposed to treadmill noise and vibration without running. Thymocytes were isolated and analyzed for phosphatidylserine (PS) externalization (Annexin V), loss of membrane integrity, mitochondria membrane depolarization, intracellular hydrogen peroxide (H(2)O(2)) production, and intracellular glutathione (GSH) as well as protein levels of caspase 3, Bcl-2, and cytosolic cytochrome c. Blood was analyzed for corticosterone (CORT) concentrations by radioimmunoassay. Exercise stress caused a significant increase in plasma CORT concentrations in EX + SAL + Imm and EX + NAC + Imm groups compared to NonEX mice. Relative to NonEX mice, thymocytes isolated from EX + SAL + Imm mice showed signs of an early apoptotic profile as indicated by decreased GSH stores and increased mitochondrial membrane depolarization. These effects were followed by a 50% reduction in thymocyte numbers 24 h post-exercise (EX + SAL + 24 h). Alterations in GSH levels, mitochondrial membrane depolarization, and thymocyte loss were not observed in mice receiving NAC. These results suggest that exercise-induced thymocyte apoptosis and cell loss may be mediated via an oxidative stress pathway.
Quadrilatero
Joe
J
Department of Health Studies and Gerontology, Faculty of Applied Health Sciences, University of Waterloo, Ont., Canada.
Hoffman-Goetz
Laurie
L
D000117:Acetylglucosamine / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D003345:Corticosterone / Q000097:blood; D045304:Cytochromes c / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D018655:Lymphocyte Count; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D010718:Phosphatidylserines / Q000378:metabolism; D005082:Physical Exertion / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology*
D017304:Annexin A5; D000975:Antioxidants; D010718:Phosphatidylserines; D019253:Proto-Oncogene Proteins c-bcl-2; D045304:Cytochromes c; D006861:Hydrogen Peroxide; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases; D005978:Glutathione; D000117:Acetylglucosamine; D003345:Corticosterone