title: HF10, an attenuated herpes simplex virus (HSV) type 1 clone, lacks neuroinvasiveness and protects mice against lethal challenge with HSV types 1 and 2.
abstract: Herpes simplex virus (HSV), a neurotropic virus, establishes life-long and, although rare, life-threatening infection in humans, and it may precipitate substantial medical and psychosocial morbidity. Here we show that HSV-1 strain HF clone 10 (HF10) exhibits impaired neuroinvasiveness in peripheral olfactory, vomeronasal and trigeminal conduits following intranasal as well as corneal inoculation. HF10 attenuation likely arises from multiple defects of HSV genes, so that HF10 will not revert to a virulent phenotype. Intranasal vaccination of mice with HF10 conferred significant protection against lethal challenge with HSV-1 and HSV-2 via the intranasal and intravaginal routes. Thus, we propose that HF10 explicitly meets the prerequisites for a candidate live attenuated HSV vaccine.
authors.affiliation: Department of Microbiology and Immunology, Aichi Medical University School of Medicine, 480-1195, Japan. isamor@aichi-med-u.ac.jp
authors.forename: Isamu
authors.initials: I
authors.lastname: Mori
authors.forename: Beixing
authors.initials: B
authors.lastname: Liu
authors.forename: Fumi
authors.initials: F
authors.lastname: Goshima
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Ito
authors.forename: Naoki
authors.initials: N
authors.lastname: Koide
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Yoshida
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokochi
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kimura
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nishiyama
mesh_terms: D000818:Animals; D001835:Body Weight; D004195:Disease Models, Animal; D005260:Female; D006561:Herpes Simplex / Q000473:pathology / Q000517:prevention & control*; D022301:Herpes Simplex Virus Vaccines / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000276:immunology* / Q000472:pathogenicity*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000821:virology*; D009832:Olfactory Nerve / Q000821:virology; D014276:Trigeminal Nerve / Q000821:virology; D014613:Vaccines, Attenuated / Q000276:immunology; D019147:Vomeronasal Organ / Q000821:virology
chemical_list: D022301:Herpes Simplex Virus Vaccines; D014613:Vaccines, Attenuated