title: Mitochondrial membrane permeabilization produced by PTP, Bax and apoptosis: a 1H-NMR relaxation study.
abstract: To analyze the involvement of structured water (bound to macromolecules) in apoptosis-induced mitochondrial outer-membrane permeability, we compared the dynamics of water protons from nuclear magnetic resonance (NMR) data in apoptotic liver mitochondria with that of control mitochondria incubated in vitro with free Ca(2+) (opening of the permeability transition pore, PTP) or with Bax alpha. Our results demonstrate that water molecules in apoptotic mitochondria exhibit an accelerated translational motion of structured water common with that induced by the opening of the PTP, but limited in amplitude. On the other hand, no significant quantitative change in structured water was observed in apoptotic mitochondria, a phenomenon also observed with Bax alpha-induced permeability. We conclude that the changes observed in the different water phases differ both quantitatively and qualitatively during the opening of the PTP and the Bax alpha-induced permeability, and that the apoptotic mitochondria exhibit mixed properties between these model situations.
authors.affiliation: Inserm UMR 601, Equipe 4, Institut de Biologie, 9 Quai Moncousu, 44035 Nantes cédex, France. daniel.pouliquen@univ-nantes.fr
authors.forename: D
authors.initials: D
authors.lastname: Pouliquen
authors.forename: G
authors.initials: G
authors.lastname: Bellot
authors.forename: G
authors.initials: G
authors.lastname: Guihard
authors.forename: P
authors.initials: P
authors.lastname: Fichet
authors.forename: K
authors.initials: K
authors.lastname: Meflah
authors.forename: F M
authors.initials: FM
authors.lastname: Vallette
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002118:Calcium / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000502:physiology / Q000648:ultrastructure; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008933:Mitochondrial Swelling; D010539:Permeability / Q000187:drug effects; D051381:Rats; D013696:Temperature; D050994:Voltage-Dependent Anion Channels / Q000187:drug effects / Q000494:pharmacology / Q000502:physiology; D014867:Water / Q000502:physiology; D051028:bcl-2-Associated X Protein / Q000494:pharmacology*
chemical_list: D050994:Voltage-Dependent Anion Channels; D051028:bcl-2-Associated X Protein; D014867:Water; D002118:Calcium