title: [The influence of ionizing radiation on the development of posterior capsule opacification in vitro].
abstract: OBJECTIVE
  Histologically, the posterior capsule opacification (PCO) corresponds to regenerative tissue of transformed lens epithelial cells (LECs) with extracellular matrix production. In this study, the influence of ionizing radiation on proliferating LECs and the development of PCO was investigated in vitro.
  
  
  METHODS
  Each four and 14 pork lenses, respectively, were irradiated with 6 MeV electrons with single doses of 8, 10, 12, and 20 Gy. 1-2 h after irradiation the lens was removed by capsulorrhexis and hydrodissection. After fixation of the capsular bag in a special device the proliferation of residual LECs was examined daily. The experiment was considered to be finished when the capsular bag was completely opacified by confluent cell proliferates.
  
  
  RESULTS
  Single dose irradiation with electrons in a dose range from 8 to 12 Gy significantly protracted the development of PCO with complete inhibition of PCO after application of 20 Gy.
  
  
  CONCLUSIONS
  To inhibit PCO in vitro, a single dose of 20 Gy is necessary. The actual in vitro model allows an optimal investigation of PCO formation under different external influences and is therefore very suitable for radiobiological questions.
authors.affiliation: Klinik für Strahlentherapie, Universitätsklinik, Regensburg. barbara.dietl@klinik.uni-regensburg.de
authors.forename: Barbara
authors.initials: B
authors.lastname: Dietl
authors.forename: Sascha
authors.initials: S
authors.lastname: Hunner
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Herrmann
authors.forename: Jörg
authors.initials: J
authors.lastname: Marienhagen
authors.forename: Michael
authors.initials: M
authors.lastname: Müller
authors.forename: Chris
authors.initials: C
authors.lastname: Lohmann
authors.forename: Veit-Peter
authors.initials: VP
authors.lastname: Gabel
mesh_terms: D000818:Animals; D002386:Cataract / Q000473:pathology / Q000517:prevention & control*; D002478:Cells, Cultured; D003627:Data Interpretation, Statistical; D004847:Epithelial Cells / Q000166:cytology / Q000528:radiation effects; D005109:Extracellular Matrix; D007903:Lens Capsule, Crystalline / Q000166:cytology / Q000473:pathology / Q000528:radiation effects*; D011829:Radiation Dosage; D011853:Radiobiology; D013552:Swine; D013997:Time Factors