Isolation, purification and structural investigation of a water-soluble polysaccharide from Solanum lyratum Thunb.
Polysaccharides, extracted from the herbs of Chinese natural Solanum lyratum Thunb (SLT), which is a traditional Chinese medicine with hot water and 0.04 M sodium hydroxide successively, were fractionated and purified by ion-exchange and gel-filtration chromatography. According to methylation, periodate oxidation, NMR spectroscopy, partial and graded acid and enzymic hydrolysis analysis, the results indicated the D-glucan to be linear and to contain both (1-->3)- and (1-->4)-linkages. The anomeric NMR and IR spectra measurements confirmed that the sugar residues were beta-glycosidically linked.
Yalin
Wu
W
Department of Chemistry, Zhejiang University, Hangzhou 310027, PR China.
Yuanjiang
Pan
P
Cuirong
Sun
S
D002236:Carbohydrate Conformation; D002498:Centrifugation; D002845:Chromatography; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004365:Drugs, Chinese Herbal; D005936:Glucans / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D046911:Macromolecular Substances / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D010100:Oxygen / Q000737:chemistry; D010504:Periodic Acid / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000737:chemistry; D032322:Solanum; D013055:Spectrophotometry, Infrared; D014867:Water / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D005936:Glucans; D046911:Macromolecular Substances; D011134:Polysaccharides; D014867:Water; D010504:Periodic Acid; D012972:Sodium Hydroxide; D005947:Glucose; D010100:Oxygen