title: Profile of differentially expressed genes after transfer of chromosome 17 into the breast cancer cell line CAL51.
abstract: Previous studies have shown that transfer of chromosome 17 suppresses the tumorigenic phenotype of the breast cancer cell line CAL51, suggesting the presence of putative tumor suppressor genes on this chromosome. Suppression subtractive hybridization and oligonucleotide microarray analyses were performed to identify differentially expressed genes in nontumorigenic microcell hybrids, CAL/17-1 and CAL/17-3, when compared with CAL51 cells. In total, 263 differentially expressed transcripts were associated with these phenotypes. Of these, a high percentage is involved in various biological processes associated with tumorigenesis, including DNA-dependent regulation of transcription, regulation of cell cycle, signal transduction, and cell proliferation. Microarray analysis of selected chromosome 17 genes in a series of 25 human primary breast tumors showed associations with clinicopathologic parameters of the tumors. Of these genes, TOB1 (transducer of ERBB2) was selected for further expression analysis. Using RT-PCR and immunohistochemical staining of tissue microarrays, we could reveal a differential mRNA and protein expression of TOB1 in the majority of breast tumors and lymph node metastases compared with normal breast tissues, indicating a potential role of this protein in breast tumorigenesis.
authors.affiliation: Institute of Pathology, University Hospital Charité, Humboldt University, D-10117 Berlin, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Klebig
authors.forename: Susanne
authors.initials: S
authors.lastname: Seitz
authors.forename: Eberhard
authors.initials: E
authors.lastname: Korsching
authors.forename: Glen
authors.initials: G
authors.lastname: Kristiansen
authors.forename: Dirk
authors.initials: D
authors.lastname: Gustavus
authors.forename: Siegfried
authors.initials: S
authors.lastname: Scherneck
authors.forename: Iver
authors.initials: I
authors.lastname: Petersen
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002886:Chromosomes, Human, Pair 17 / Q000502:physiology*; D020869:Gene Expression Profiling; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D047908:Intracellular Signaling Peptides and Proteins; D012334:RNA, Neoplasm; C098669:TOB1 protein, human; D025521:Tumor Suppressor Proteins