[Preliminary screening of specific surface markers of human spermatogonial stem cells].
OBJECTIVE
To explore the specific surface markers for the isolation and purification of human spermatogonial stem cells (SSC).


METHODS
Specific markers of human SSC were screened and identified in fetal and adult testes by immunohistochemical assay, using HSC markers c-kit, Thy-1 and human ES integrins.


RESULTS
In human adult testes, the alpha6 integrin extensively and significantly expressed on the surface of most of the germ cells in the seminiferous tubule, and beta1 integrin mainly expressed on the surface of the germ cells residing on or near the basal membrane in the seminiferous tubule. Thy-1 scattering expressed on the surface of some cells of the basal membrane, and on some Leydig cells as well. The three antigen markers expressed on the SSC of human adult testes specifically to some extent. SSEA-1 specifically expressed on the surface of the gonocytes in the fetal testes.


CONCLUSIONS
The alpha6 and beta1 integrins and Thy-1 may be used for the SSC isolation as positive markers. SSEA-1 can be used as an identification marker for the fetus SSC.
Li
Yan-feng
YF
Department of Urology, Institute of Field Surgery, Daping Hospital, the Third Military Medical University, Chongqing 400042, China. lyf1000@yahoo.com.cn
Guo
Ying-lu
YL
Li
Xiao-hong
XH
Jin
Feng-shuo
FS
Sun
Zhong-yi
ZY
Zhang
Ke-qin
KQ
D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D002454:Cell Differentiation; D005333:Fetus / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D039503:Integrin alpha6 / Q000032:analysis; D019012:Integrin beta1 / Q000032:analysis; D016256:Lewis X Antigen / Q000032:analysis; D008297:Male; D013093:Spermatogonia / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*; D013737:Testis / Q000166:cytology*; D018800:Thy-1 Antigens / Q000032:analysis
D015415:Biomarkers; D039503:Integrin alpha6; D019012:Integrin beta1; D016256:Lewis X Antigen; D018800:Thy-1 Antigens