title: [Effect of different FSB concentration and first-time exchange of total volume medium on proliferation of bone marrow stromal cells from GFP transgenic mouse in vitro].
abstract: OBJECTIVE
  To evaluate the effect of different fetal bovine serum (FBS) concentration and first-time exchange of total volume medium on the proliferation of bone marrow stromal cells (BMSCs) from green fluorescence protein (GFP) transgenic mouse in vitro.
  
  
  METHODS
  Bone marrow cells isolated from GFP transgenic mice were cultured in DMEM/F12 containing 10%, 20%, 30% FBS respectively; the first exchange of the total volume medium was made at different times (4 h, 6 h, 8 h, 10 h, 12 h, 24 h, 48 h and 72 h) after 3 d primary culture; then the total volume medicum was exchanged every three days. The amplification of BMSCs was determined. The passage 5 BMSCs cultured in DMEM/F12 containing 10% and 20% FBS were examined with the antibodies CD44, CD45 and CD54 at the time of first exchange of the total volume medium.
  
  
  RESULTS
  The cultured cells proliferated well in DMEM/F12 containing 10% FBS and 20% FBS. With the extension of the time for first exchange of total volume medium, the density of the adhered cells increased, but the purity of BMSCs decreased.
  
  
  CONCLUSIONS
  The method of making cells adhere to culture plastic in different time for cultivating and purifying BMSCs from GFP transgenic mice is effective, the appropriate concentration of FBS is 10%-20% and the best time for the the first exchange of total volume medium is 8 hour.
authors.affiliation: The Institute of Neuroscience, Kunming Medical College, Kunming 650031, China.
authors.forename: Zhen-jiang
authors.initials: ZJ
authors.lastname: Shi
authors.forename: Gui-qin
authors.initials: GQ
authors.lastname: Huang
authors.forename: Ting-hua
authors.initials: TH
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Fu-qiang
authors.initials: FQ
authors.lastname: Li
authors.forename: Zhong-tang
authors.initials: ZT
authors.lastname: Feng
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D049452:Green Fluorescent Proteins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D011897:Random Allocation; D044967:Serum; D017154:Stromal Cells / Q000166:cytology*
chemical_list: D003470:Culture Media; D049452:Green Fluorescent Proteins