Simple electrospray mass spectrometry detection of acylhomoserine lactones.
Simple (non-tandem) electrospray mass spectrometry (ESMS) can detect acyl homoserine lactones (AHL) in bacteriological media in picomole amounts. The chemical reactivity of AHLs and their extraction behaviour into ethyl acetate, coupled with detection in the ESMS, has shown that these lactones can be detected as the protonated pseudomolecular ions themselves as well as solvent and ammonium adducts, and as dimers. ESMS detects and identifies these molecules, utilizing simple chemical properties of AHLs.
Makemson
John
J
Department of Biological Sciences, Florida International University, Miami, 33199, USA. makemson@ful.edu
Eberhard
Anatol
A
Mathee
Kalai
K
D006714:Homoserine / Q000737:chemistry*; D007783:Lactones / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014733:Vibrio / Q000737:chemistry
D007783:Lactones; D006714:Homoserine