Radiation sensitivities of 31 human oesophageal squamous cell carcinoma cell lines.
The purpose of this study was to determine the radiosensitivities of 31 human oesophageal squamous cell carcinoma cell lines with a colony-formation assay. A large variation in radiosensitivity existed among 31 cell lines. Such a large variation may partly explain the poor result of radiotherapy for this cancer. One cell line (KYSE190) demonstrated an unusual radiosensitivity. Ataxia-telangiectasia-mutated (ATM) gene in these cells had five missense mutations, and ATM protein was truncated or degraded. Inability to phosphorylate Chk2 in the irradiated KYSE190 cells suggests that the ATM protein in these cells had lost its function. The dysfunctional ATM protein may be a main cause of unusual radiosensitivity of KYSE190 cells. Because the donor of these cells was not diagnosed with ataxia telangiectasia, mutations in ATM gene might have occurred during the initiation and progression of cancer. Radiosensitive cancer developed in non-hereditary diseased patients must be a good target for radiotherapy.
Ban
Sadayuki
S
Frontier Research Center, National Institute of Radiological Sciences, Anagawa, Inage-ku, Chiba 263-8555, Japan. s_ban@nirs.go.jp
Michikawa
Yuichi
Y
Ishikawa
Ken-ichi
K
Sagara
Masashi
M
Watanabe
Koji
K
Shimada
Yutaka
Y
Inazawa
Johji
J
Imai
Takashi
T
D064007:Ataxia Telangiectasia Mutated Proteins; D015153:Blotting, Western / Q000379:methods; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000532:radiotherapy*; D018797:Cell Cycle Proteins / Q000235:genetics; D045744:Cell Line, Tumor; D004260:DNA Repair / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics / Q000532:radiotherapy*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D020125:Mutation, Missense / Q000235:genetics; D009363:Neoplasm Proteins / Q000235:genetics; D009687:Nuclear Proteins; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics
D018797:Cell Cycle Proteins; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D025521:Tumor Suppressor Proteins; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases