Protective effect of membrane cofactor protein against complement-dependent injury.
OBJECTIVE
To evaluate the protective role of membrane cofactor protein (MCP, CD46) on complement-dependent injury.


METHODS
MCP was separated by ion exchange chromatography on a DEAE sephadex A-50 column from pig erythrocyte ghosts. Its protective effect was tested in models such as cobra venom factor (CVF)-induced platelet metamorphosis and aggregation, human serum-induced injury in isolated working guinea pig heart and reverse passive Arthus reaction.


RESULTS
MCP inhibited CVF-induced platelet metamorphosis with an IC50 of 56.7 mg/L+/-2.6 mg/L, and prevented injury induced by activated complement in isolated working guinea pig hearts. In the rat model of reverse Arthus reaction, MCP relieved the skin lesions induced by immune complexes.


CONCLUSIONS
MCP has a protective effect against complement-dependent injury.
Xu
Dong
D
Department of Pharmacology, Sun Yat-Sen Medical College of Sun Yat-Sen University, Guangzhou 510080, China.
Huang
Shou-jian
SJ
Wang
Jin-qun
JQ
Wu
Chu-kun
CK
D000818:Animals; D001183:Arthus Reaction / Q000276:immunology / Q000517:prevention & control; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D003165:Complement System Proteins / Q000494:pharmacology / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004546:Elapid Venoms / Q000494:pharmacology; D004912:Erythrocytes / Q000737:chemistry; D005260:Female; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D051916:Membrane Cofactor Protein / Q000302:isolation & purification / Q000494:pharmacology*; D020556:Neutrophil Infiltration / Q000187:drug effects / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D010974:Platelet Aggregation / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012756:Sheep; D012867:Skin / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D013552:Swine; D015054:Zymosan / Q000494:pharmacology
D004546:Elapid Venoms; D051916:Membrane Cofactor Protein; C016310:cobra venom factor; D010047:Ovalbumin; D003165:Complement System Proteins; D015054:Zymosan