[A polymorphism of kynureninase gene in a hypertensive candidate chromosomal region is associated with essential hypertension].
OBJECTIVE
To identify single nucleotide polymorphisms (SNP) in the regulatory and coding regions of human kynureninase (KYNU) gene in a hypertensive candidate chromosomal region 2q14-q23 of Han Chinese, and to investigate the relationship between polymorphisms in KYNU and essential hypertension.


METHODS
The SNPs in the promoter region and exons of the KYNU gene were detected by direct DNA sequencing. Genotyping of the nonsynonymous Lys412Glu (A/G) polymorphism was performed by DHPLC technology in 456 hypertensive patients and 430 normal controls.


RESULTS
Sixteen SNP were identified in the KYNU gene, including 6 in the regulatory region and 2 in the coding region (both of them lead to substitution of amino acid). Significant differences between hypertensive patients and normal controls were observed for the distribution of alleles (chi(2) = 6.693, P = 0.035) and genotypes (chi(2) = 4.188, P = 0.041) of the Lys412Glu polymorphism in all subjects, and for the distribution of alleles in the subgroup of men (chi(2) = 4.424, P = 0.035).


CONCLUSIONS
The Lys412Glu polymorphism of the KYNU gene in a hypertensive candidate chromosomal region is associated with essential hypertension in Han Chinese.
Zhang
Yi
Y
Rui Jin Hospital, Shanghai Institute of Hypertension, State Key Laboratory of Medical Genomics, Shanghai Key Laboratory of Vascular Biology, Shanghai Second Medical University, Shanghai 200025, China.
Zhang
Kui-xing
KX
He
Xin
X
Yuan
Wen-tao
WT
Wang
Gu-liang
GL
Mao
Shou-yu
SY
Gao
Ping-jin
PJ
Huang
Wei
W
Zhu
Ding-liang
DL
D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D005091:Exons; D005260:Female; D005787:Gene Frequency; D006801:Humans; D006867:Hydrolases / Q000235:genetics*; D006973:Hypertension / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
D006867:Hydrolases; C021480:kynureninase