title: Tobamovirus-resistant tobacco generated by RNA interference directed against host genes.
abstract: Two homologous Nicotiana tabacum genes NtTOM1 and NtTOM3 have been identified. These genes encode polypeptides with amino acid sequence similarity to Arabidopsis thaliana TOM1 and TOM3, which function in parallel to support tobamovirus multiplication. Simultaneous RNA interference against NtTOM1 and NtTOM3 in N. tabacum resulted in nearly complete inhibition of the multiplication of Tomato mosaic virus and other tobamoviruses, but did not affect plant growth or the ability of Cucumber mosaic virus to multiply. As TOM1 and TOM3 homologues are present in a variety of plant species, their inhibition via RNA interference should constitute a useful method for generating tobamovirus-resistant plants.
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Momoko
authors.initials: M
authors.lastname: Asano
authors.forename: Rena
authors.initials: R
authors.lastname: Satoh
authors.forename: Atsuko
authors.initials: A
authors.lastname: Mochizuki
authors.forename: Shinya
authors.initials: S
authors.lastname: Tsuda
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nishiguchi
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Hirai
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Meshi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Naito
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishikawa
mesh_terms: D000595:Amino Acid Sequence; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D034622:RNA Interference; D014026:Nicotiana / Q000235:genetics* / Q000821:virology*; D017898:Tobamovirus / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics / Q000502:physiology