Expression of IGF-II mRNA-binding proteins (IMPs) in gonads and testicular cancer.
Insulin-like growth factor-II mRNA-binding proteins 1, 2 and 3 (IMP1, IMP2 and IMP3) belong to a family of RNA-binding proteins implicated in mRNA localization, turnover and translational control. We examined their expression pattern during development of murine and human testis and ovaries. In the mouse, IMPs were expressed in male and female gonadal cells at embryonic day 12.5 (E12.5). From E16.5, IMP1 and IMP3 became restricted to the developing germ cells, whereas IMP2 expression persisted in the interstitial cells. In mature mouse and human ovaries, IMP1, IMP2 and IMP3 were detected in resting and growing oocytes and in the granulosa cells. In testis, IMP1 and IMP3 were found mainly in the spermatogonia, whereas IMP2 was expressed in the immature Leydig cells. Moreover, all three IMPs were detected in human semen. The developmental expression pattern of IMP1 and IMP3 in the human testis prompted us to examine their possible involvement in testicular neoplasia. IMPs were detected primarily in germ-cell neoplasms, including preinvasive testicular carcinoma in situ, classical and spermatocytic seminoma, and nonseminomas, with particularly high expression in undifferentiated embryonal carcinoma. The relative expression of IMP1, IMP2 and IMP3 varied among tumor types and only IMP1 was detected in all carcinoma in situ cells. Thus IMPs, and in particular IMP1, may be useful auxiliary markers of testicular neoplasia.
Hammer
Niels A
NA
Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark.
Hansen
Thomas v O
Tv
Byskov
Anne Grete
AG
Rajpert-De Meyts
Eva
E
Grøndahl
Marie Louise
ML
Bredkjaer
Helle E
HE
Wewer
Ulla M
UM
Christiansen
Jan
J
Nielsen
Finn C
FC
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D001755:Blastocyst / Q000737:chemistry; D015153:Blotting, Western / Q000379:methods; D002278:Carcinoma in Situ / Q000737:chemistry; D018236:Carcinoma, Embryonal / Q000737:chemistry; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D005865:Gestational Age; D006066:Gonads / Q000737:chemistry*; D006107:Granulosa Cells / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D007985:Leydig Cells / Q000737:chemistry; D008297:Male; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis; D009373:Neoplasms, Germ Cell and Embryonal / Q000737:chemistry*; D009865:Oocytes / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000032:analysis*; D012661:Semen / Q000737:chemistry; D018239:Seminoma / Q000737:chemistry; D013094:Spermatozoa / Q000737:chemistry; D013736:Testicular Neoplasms / Q000737:chemistry*; D013737:Testis / Q000737:chemistry
D014408:Biomarkers, Tumor; C406144:IGF2BP1 protein, human; C498779:IGF2BP2 protein, human; C498780:IGF2BP3 protein, human; D009363:Neoplasm Proteins; D016601:RNA-Binding Proteins; D007335:Insulin-Like Growth Factor II