title: Susceptibility to oxidative stress of mussels (Mytilus galloprovincialis) in the Venice Lagoon (Italy).
abstract: The aim of this study was to evaluate the susceptibility to pollutant mediated oxidative stress of the Mediterranean mussel Mytilus galloprovincialis in the Venice lagoon (Italy). In June 2003, mussels from a farm were transplanted to eight sites in the lagoon for five weeks. Oxidative stress responses were measured by: (i) total oxyradical scavenging capacity (TOSC) assay, for an overall evaluation of the oxidative stress response capability; (ii) catalase (CAT), as a key enzyme involved in the antioxidant defence system; (iii) malondialdehyde (MDA), as an indicator of lipid peroxidation, to evaluate an oxidative damage; (iv) metallothioneins (MTs), as they play a role in the antioxidant defence. The TOSC analysis revealed a reduced capability to eliminate: (i) peroxyl radical in mussels transplanted at Palude della Rosa, Valle Millecampi and Chioggia; (ii) hydroxyl radical at Campalto and Valle Millecampi; (iii) peroxynitrite at Valle Millecampi. Inhibition in CAT activity, observed in all the monitored sites, confirms the presence of an oxidative pressure in transplanted mussels. In addition, Pearson correlation analysis was performed in order to observe possible links between the various parameters. The PCA was a powerful tool to discriminate impacted sites, suggesting that the mussels transplanted throughout the Venice lagoon were subjected to different levels of oxidative pressure. Furthermore, it provided an easy and useful tool to summarize the obtained results.
authors.affiliation: Institute of Marine Science, ISMAR-CNR, Castello 1363/a, Venice, Italy.
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Pampanin
authors.forename: Lionel
authors.initials: L
authors.lastname: Camus
authors.forename: Alessio
authors.initials: A
authors.lastname: Gomiero
authors.forename: Ilenia
authors.initials: I
authors.lastname: Marangon
authors.forename: Elisa
authors.initials: E
authors.lastname: Volpato
authors.forename: Cristina
authors.initials: C
authors.lastname: Nasci
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D002374:Catalase / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods; D016166:Free Radical Scavengers / Q000737:chemistry; D006458:Hemolymph / Q000737:chemistry; D017665:Hydroxyl Radical / Q000032:analysis; D007558:Italy; D008315:Malondialdehyde / Q000032:analysis; D008668:Metallothionein / Q000032:analysis; D049878:Mytilus / Q000737:chemistry / Q000201:enzymology / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D010545:Peroxides / Q000032:analysis; D030421:Peroxynitrous Acid / Q000032:analysis; D025341:Principal Component Analysis; D013223:Statistics as Topic
chemical_list: D015415:Biomarkers; D016166:Free Radical Scavengers; D010545:Peroxides; D030421:Peroxynitrous Acid; C049375:perhydroxyl radical; D017665:Hydroxyl Radical; D008315:Malondialdehyde; D008668:Metallothionein; D002374:Catalase