title: Evaluation of diphenylamine derivatives in apple peel using gradient reversed-phase liquid chromatography with ultraviolet-visible absorption and atmospheric pressure chemical ionization mass selective detection.
abstract: A method was developed for extracting, identifying, and quantifying diphenylamine (DPA) derivatives in the peel of DPA-treated apples using gradient reversed-phase liquid chromatography with ultraviolet-visible absorption and atmospheric pressure chemical ionization detection (LC-UV-vis-APCI-MS). Compounds routinely analyzed using this method included hydroxylated, nitrosated, nitrated, and methoxylated diphenylamine derivatives. Analysis of peel treated with 0-8 g L(-1) DPA showed that peel DPA content was a limiting factor in derivative production and that recovery of most compounds over this range was linear.
authors.affiliation: Tree Fruit Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 1104 N. Western Ave., Wenatchee, WA 98801, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Rudell
authors.forename: James P
authors.initials: JP
authors.lastname: Mattheis
authors.forename: John K
authors.initials: JK
authors.lastname: Fellman
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004159:Diphenylamine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000494:pharmacology; D027845:Malus / Q000737:chemistry* / Q000187:drug effects; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000975:Antioxidants; D004159:Diphenylamine