title: The glycopeptide CSF114(Glc) detects serum antibodies in multiple sclerosis.
abstract: Synthetic glycopeptides have the potential to detect antibodies in multiple sclerosis (MS). In the present study, we analyzed the antibodies (IgM class, IgG class and IgG subclasses) to the synthetic glycopeptide CSF114(Glc) in the serum of 186 MS patients, 166 blood donors (BDs), 25 patients affected by meningitis/encephalitis, 41 affected by systemic lupus erythematosus (SLE) and 49 affected by rheumatoid arthritis (RA). The IgM antibody level to CSF114(Glc) was significantly increased in MS patients versus BDs (p<0.001) or versus other autoimmune diseases (SLE or RA, p<0.001). The IgG response was restricted to the subclass IgG2. IgM antibodies to CSF114(Glc) were found in 30% of relapsing/remitting MS patients and, at lower levels, in subjects affected by meningitis/encephalitis. The study of antibodies to CSF114(Glc) is a new, potential immunological marker of MS.
authors.affiliation: Dipartimento di Scienze Neurologiche e Psichiatriche, Università degli Studi di Firenze, viale Morgagni 85, I-50134 Firenze, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Lolli
authors.forename: Benedetta
authors.initials: B
authors.lastname: Mazzanti
authors.forename: Marta
authors.initials: M
authors.lastname: Pazzagli
authors.forename: Elisa
authors.initials: E
authors.lastname: Peroni
authors.forename: Maria Claudia
authors.initials: MC
authors.lastname: Alcaro
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Sabatino
authors.forename: Roberta
authors.initials: R
authors.lastname: Lanzillo
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Brescia Morra
authors.forename: Lucio
authors.initials: L
authors.lastname: Santoro
authors.forename: Claudio
authors.initials: C
authors.lastname: Gasperini
authors.forename: Stefania
authors.initials: S
authors.lastname: Galgani
authors.forename: Mario Milco
authors.initials: MM
authors.lastname: D'Elios
authors.forename: Valentina
authors.initials: V
authors.lastname: Zipoli
authors.forename: Stefano
authors.initials: S
authors.lastname: Sotgiu
authors.forename: Maura
authors.initials: M
authors.lastname: Pugliatti
authors.forename: Paolo
authors.initials: P
authors.lastname: Rovero
authors.forename: Mario
authors.initials: M
authors.lastname: Chelli
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Papini
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D000906:Antibodies / Q000097:blood* / Q000145:classification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006020:Glycopeptides / Q000138:chemical synthesis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood* / Q000145:classification / Q000175:diagnosis / Q000276:immunology; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D006020:Glycopeptides