[Optimization study on extraction technology of the seed of Ziziphus jujuba var. spinosa by orthogonal design with multi-targets].
OBJECTIVE
To optimize extraction technology of the seed of Ziziphus jujuba var. spinosa with the targets of the total saponin, total jujuboside A and B and total flavonoids.


METHODS
In the method of one-way and orthogonal tests, ethanol concentration, amount of ethanol, extraction time and extraction times were the factors in orthogonal test, and each factor with three levels.


RESULTS
Ethanol concentration and extraction times had significant effect on all the targets, other factors should be selected in accordance with production practice.


CONCLUSIONS
The best extraction technology is to extract for three times with 8 fold ethanol solution (60%), and 1.5 h each time.
Wang
Xiao-liang
XL
Department of Chinese Pharmacy , Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
Zhang
Yu-jie
YJ
Chen
Ming-xia
MX
Wang
Ze-feng
ZF
D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol; D047309:Flavones / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000032:analysis; D012639:Seeds / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D031957:Ziziphus / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D047309:Flavones; D012503:Saponins; D000431:Ethanol; C087808:jujuboside A