Oral exposure to Microcystis increases activity-augmented antioxidant enzymes in the liver of loach (Misgurnus mizolepis) and has no effect on lipid peroxidation.
Recently, eutrophication has induced severe cyanobacterial blooms in the Naktong River, the second largest river of Korea. In the present study, lipid peroxidation and the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, were evaluated in the liver of loach (Misgurnus mizolepis) that were orally exposed to a low dose of Microcystis through dietary supplementation with bloom scum. Loach received 75 mg of dry cells/kg body weight mass (equal to 10 microg microcystin-RR/kg body mass), for 28 days under controlled conditions. Antioxidant enzymatic activity and lipid peroxidation were measured after termination of exposure. The activities of antioxidant enzyme were significantly increased in the livers of toxin-exposed loach after 28 days of exposure, as compared to control fish. However, lipid peroxidation remained stable in both groups. These results suggest that antioxidant enzymes were able to eliminate oxidative stress induced by low concentrations of microcystins and to prevent increased lipid peroxidation in the liver of loach.
Li
Xiao-Yu
XY
School of Life Science, Henan Normal University, XinXiang 453007, People's Republic of China.
Chung
Ik-Kyo
IK
Kim
Jung-In
JI
Lee
Jin-Ae
JA
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001427:Bacterial Toxins / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D003531:Cypriniformes / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D052998:Microcystins; D046931:Microcystis / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010456:Peptides, Cyclic / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D001427:Bacterial Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase