title: SCAR markers linked to the common bean rust resistance gene Ur-13.
abstract: Rust in common bean (Phaseolus vulgaris L.) is caused by Uromyces appendiculatus Pers.:Pers. (Unger) which exhibits a high level of pathogenic diversity. Resistance to this disease is conditioned by a considerable number of genes. Pyramiding resistance genes is desirable and could be simplified by the use of molecular markers closely linked to the genes. The resistance gene Ur-13, present in the South African large seeded cultivar Kranskop, has been used extensively in the local breeding program. The purpose of this study was the development of a molecular marker linked to Ur-13. An F(2) population derived from a cross between Kranskop and a susceptible (South African) cultivar Bonus was used in combination with bulked segregant analysis utilizing the amplified fragment length polymorphism (AFLP) technique. Seven AFLP fragments linked significantly to the rust resistance and five were successfully converted to sequence characterized amplified region (SCAR) markers. The co-dominant SCAR markers derived from a 405 bp EAACMACC fragment, KB 126, was located 1.6 cM from the gene. Two additional SCAR markers and one cleaved amplified polymorphic sequence marker were located further from the gene. The gene was mapped to linkage group B8 on the BAT 93/Jalo EEP 558 core map (chromosome 3).
authors.affiliation: ARC-Grain Crops Institute, Private Bag X1251, Potchefstroom 2520, South Africa. MienieC@arc.agric.za
authors.forename: C M S
authors.initials: CM
authors.lastname: Mienie
authors.forename: M M
authors.initials: MM
authors.lastname: Liebenberg
authors.forename: Z A
authors.initials: ZA
authors.lastname: Pretorius
authors.forename: P N
authors.initials: PN
authors.lastname: Miklas
mesh_terms: D001483:Base Sequence; D001487:Basidiomycota / Q000472:pathogenicity*; D003433:Crosses, Genetic; D005784:Gene Amplification; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D027805:Phaseolus / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers