title: Alteration of striatal [11C]raclopride and 6-[18F]fluoro-L-3,4-dihydroxyphenylalanine uptake precedes development of methamphetamine-induced rotation following unilateral 6-hydroxydopamine lesions of medial forebrain bundle in rats.
abstract: We studied the positron emission tomography (PET) tracer distributions of ligands for dopamine D1 receptors ([11C]SCH23390) and D2 receptors ([11C]raclopride) and of the dopamine precursor analog 6-[18F]fluoro-L-3,4-dihydroxyphenylalanine ([18F]FDOPA) in the brain after 6-hydroxydopamine (6-OHDA) lesions of the medial forebrain bundle in rats. The number of methamphetamine-induced rotation was higher at 14 days than at 3 days after the 6-OHDA lesions. The brains of 6-OHDA-treated rats were analyzed by tissue dissection following i.v. bolus of each tracer at 3 days (acute stage) or 3 weeks (chronic stage) postlesion. [11C]Raclopride, but not [11C]SCH23390, showed higher accumulation in the striatum on the lesion side than on the non-lesion (intact) side both at 3 days and 3 weeks postlesion. On the other hand, lower accumulation of [18F]FDOPA was observed in the striatum on the lesion side at 3 days postlesion and in both the striatum and cerebral cortex on the lesion side at 3 weeks postlesion. Our studies demonstrate that an increase in [11C]raclopride and a decrease in [18F]FDOPA uptake in the denervated striatum is evident even at 3 days after the 6-OHDA lesions when the methamphetamine-induced rotational behavior is not established.
authors.affiliation: Department of Psychiatry, Miyazaki Medical College, University of Miyazaki, 5200 Kihara, Miyazaki-gun, Miyazaki 889-1692, Japan. ishiday@med.miyazaki-u.ac.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ishida
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kawai
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Magata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Yoshimoto
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Takeda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hashiguchi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Mukai
authors.forename: Hideo
authors.initials: H
authors.lastname: Saji
mesh_terms: D000818:Animals; D001692:Biological Transport; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D005462:Fluorine Radioisotopes / Q000493:pharmacokinetics; D007839:Functional Laterality; D008694:Methamphetamine / Q000494:pharmacology*; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology*; D016627:Oxidopamine / Q000633:toxicity*; D016548:Prosencephalon / Q000000981:diagnostic imaging* / Q000187:drug effects / Q000473:pathology; D020891:Raclopride / Q000493:pharmacokinetics*; D011877:Radionuclide Imaging; D051381:Rats; D012399:Rotation; D014018:Tissue Distribution
chemical_list: D002250:Carbon Radioisotopes; D005462:Fluorine Radioisotopes; C043437:fluorodopa F 18; D020891:Raclopride; D008694:Methamphetamine; D004295:Dihydroxyphenylalanine; D016627:Oxidopamine