[Urinary concentration of proinflammatory cytokines with regard to infected urinary tract region].
Uropathogenic bacteria stimulate epithelial cells of interstitial tissue and macrophages to secrete proinflammatory cytokines: interleukin I (IL-1beta), interleukin 6 (IL-6) and interleukin 8 (IL-8). The aim of the study was to check: 1) if the concentration of proinflammatory cytokines (IL-1beta, IL-6, IL-8) differs in dependence on region and clinical picture of urinary tract infection, 2) what is the influence of antibacterial treatment on their concentration.


METHODS
We examined 67 children, aged 1-15 years, who were divided into 3 groups: 27 children with acute pyelonephritis (AP), caused by E. coli (group I), in whom the examination was carried out twice: A - before treatment, B - after 14 days of antibacterial treatment, 10 children with chronic urinary tract infection (UTI) associated with neurogenic bladder (group II) and 30 healthy children (group K).


METHODS
Urinary concentration of examined cytokines was assessed using ELISA immunoenzymatic method and was expressed in pg/mg creatinine. Results showed that in group I before treatment the urinary concentration of examined cytokines was increased (p<0.05). After antibacterial treatment concentration of IL-1beta was normal and concentration of IL-6 and IL-8 decreased but was still higher than in control group (p<0.05). In group II before treatment the increase in concentration of IL-1beta and IL-8 was not so high (p<0.05) and the urinary concentration of IL-6 was normal (p>0.05). In examination A in children from group I and II a positive correlation between examined cytokines and C reactive protein was shown. We have also found a positive correlation between urinary concentration of IL-1beta a IL-8.


CONCLUSIONS
1. Urinary concentration of examined proinflammatory cytokines is different in children with AP and UTI associated with neurogenic bladder and correlates with concentration of C-reactive protein. 2. In most of children with AP after 14-days of antibacterial treatment the urinary concentration of proinflammatory cytokines has been increased.
Kassem
Jamal Ai-din M
JA
Z I Kliniki Chorób Dzieci Akademii Medycznej w Białymstoku.
Wasilewska
Anna M
AM
Zoch-Zwierz
Walentyna M
WM
D000293:Adolescent; D000704:Analysis of Variance; D002097:C-Reactive Protein / Q000652:urine; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D007375:Interleukin-1 / Q000652:urine; D015850:Interleukin-6 / Q000652:urine; D016209:Interleukin-8 / Q000652:urine; D008297:Male; D011044:Poland; D011704:Pyelonephritis / Q000188:drug therapy / Q000276:immunology* / Q000382:microbiology / Q000652:urine; D012307:Risk Factors; D018709:Statistics, Nonparametric; D001750:Urinary Bladder, Neurogenic / Q000188:drug therapy / Q000276:immunology* / Q000382:microbiology / Q000652:urine; D014552:Urinary Tract Infections / Q000188:drug therapy / Q000276:immunology* / Q000382:microbiology / Q000652:urine
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D002097:C-Reactive Protein