p53 intronic G13964C variant in colon cancer and its association with HPV.
BACKGROUND
Inactivation of p53 is the most common change identified in human cancer. Nucleotide alterations in p53 intron 6 have been reported to be associated with the dysregulation of p53 function and tumor development. The aim of our study was to assess whether the intron 6 G13964C variant of the p53 gene is associated with the presence of human papillomavirus (HPV) as a risk factor in colon cancer.


METHODS
To determine whether the intronic G13964C alteration is involved in colon carcinogenesis, surgical specimens from 55 patients with colon cancer and HPV-positive tumors were examined by PCR-RFLP. Blood samples from 77 healthy subjects were used as the control group.


RESULTS
The 13964C variant was found in 14.5% of colon cancer patients and 12.9% of the control subjects. One patient and two controls were homozygous for this base change.


CONCLUSIONS
No evidence of a significant association between the p53 G13964C allele and colon carcinoma was found.
Buyru
Nur
N
I. U. Cerrahpasa Medical Faculty, Department of Medical Biology, Temel Bilimler Binasi, Kat 6 Kocamustafapasa, Istanbul.
Tezol
Ayda
A
Dalay
Nejat
N
D000483:Alleles; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000235:genetics* / Q000821:virology*; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007438:Introns; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000150:complications*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length