Anti-CCP: history and its usefulness.
Antibodies directed to cyclic citrullinated peptides (anti-CCP) are highly specific for rheumatoid arthritis (RA) and can easily be detected in sera by using commercially available immunoassays. The second version of the anti-CCP test (anti-CCP2) demonstrated high specificity (89-98%) and good sensitivity (41-88%) for RA. Commercially available ELISA methods from three different companies are on the market. All three CCP2 assays show similar results as all CCP2 assays use the same antigen-coated plates. This study was an evaluation of a new automated method for the determination of anti-CCP2 in a routine laboratory setting. Five hundred and forty three serum samples were tested for anti-CCP2 within normal routine diagnostic using a commercially available ELISA and retested with a prelaunch version of a new and fully-automated method (EliA). The results were comparable. The new automated assay is easy to use and demonstrated a diagnostic sensitivity of 80% and specificity of 97%.
Herold
M
M
Rheumatology Unit, Clinical Department of General Internal Medicine, Innsbruck Medical University, A-6020 Innsbruck, Austria. manfred.herold@uibk.ac.at
Boeser
V
V
Russe
E
E
Klotz
W
W
D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000175:diagnosis* / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000706:statistics & numerical data; D005260:Female; D006801:Humans; D008297:Male; D010456:Peptides, Cyclic / Q000276:immunology*; D012680:Sensitivity and Specificity
D001323:Autoantibodies; D010456:Peptides, Cyclic; C487763:cyclic citrullinated peptide