Probiotics reduce enterohemorrhagic Escherichia coli O157:H7- and enteropathogenic E. coli O127:H6-induced changes in polarized T84 epithelial cell monolayers by reducing bacterial adhesion and cytoskeletal rearrangements.
The aim of this study was to determine if probiotics reduce epithelial injury following exposure to Escherichia coli O157:H7 and E. coli O127:H6. The pretreatment of intestinal (T84) cells with lactic acid-producing bacteria reduced the pathogen-induced drop in transepithelial electrical resistance. These findings demonstrate that probiotics prevent epithelial injury induced by attaching-effacing bacteria.
Sherman
Philip M
PM
Gastroenterology and Nutrition, Room 8409, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada. sherman@sickkids.ca
Johnson-Henry
Kathene C
KC
Yeung
Helen P
HP
Ngo
Peter S C
PS
Goulet
Jacques
J
Tompkins
Thomas A
TA
D000185:Actinin / Q000378:metabolism; D001422:Bacterial Adhesion / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology; D004927:Escherichia coli Infections / Q000378:metabolism / Q000628:therapy; D019453:Escherichia coli O157 / Q000378:metabolism*; D006801:Humans; D007779:Lactobacillus acidophilus / Q000378:metabolism; D019936:Probiotics / Q000378:metabolism*
D000185:Actinin