A genome-wide set of congenic mouse strains derived from DBA/2J on a C57BL/6J background.
In the analysis of complex traits, congenic strains are powerful tools because they allow characterization of a single locus in the absence of genetic variation throughout the remainder of the genome. Here, we report the construction and initial characterization of a genome-wide panel of congenic strains derived from the donor strain DBA/2J on the background strain C57BL/6J. For many strains, we have carried out high-density SNP genotyping to precisely map the congenic interval and to identify any contaminating regions. Certain strains exhibit striking variation in litter size and in the ratio of females to males. We illustrate the utility of the set by "Mendelizing" the complex trait of myocardial calcification. These 65 strains cover more than 95% of the autosomal genome and should facilitate the analysis of the many genetic trait differences that have been reported between these parental strains.
Davis
Richard C
RC
Department of Microbiology, Immunology and Molecular Genetics, Department of Medicine, and Department of Human Genetics, University of California, Los Angeles, CA 90095-1679, USA. davisr@ucla.edu
Schadt
Eric E
EE
Smith
Desmond J
DJ
Hsieh
Elena W Y
EW
Cervino
Alessandra C L
AC
van Nas
Atila
A
Rosales
Melenie
M
Doss
Sudheer
S
Meng
Haijin
H
Allayee
Hooman
H
Lusis
Aldons J
AJ
D000818:Animals; D005260:Female; D016678:Genome; D023281:Genomics; D005838:Genotype; D008297:Male; D051379:Mice; D020297:Mice, Congenic / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci