title: An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants.
abstract: A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.
authors.affiliation: Institute of Chemistry, University of Potsdam, Germany. schmaelzlin@chem.uni-potsdam.de
authors.forename: Elmar
authors.initials: E
authors.lastname: Schmälzlin
authors.forename: Joost T
authors.initials: JT
authors.lastname: van Dongen
authors.forename: Ingo
authors.initials: I
authors.lastname: Klimant
authors.forename: Bettina
authors.initials: B
authors.lastname: Marmodée
authors.forename: Martin
authors.initials: M
authors.lastname: Steup
authors.forename: Joachim
authors.initials: J
authors.lastname: Fisahn
authors.forename: Peter
authors.initials: P
authors.lastname: Geigenberger
authors.forename: Hans-Gerd
authors.initials: HG
authors.lastname: Löhmannsröben
mesh_terms: D044784:Chara / Q000378:metabolism*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007424:Intracellular Fluid / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010100:Oxygen / Q000032:analysis*; D010101:Oxygen Consumption / Q000502:physiology*; D010944:Plants / Q000378:metabolism*
chemical_list: D010100:Oxygen