title: Diagnostic aspects of organophosphate poisoning.
abstract: Organophosphate (OP)-type chemical warfare agents (nerve agents) present a constant threat to the population. Sensitive and specific methods for the detection and verification of exposure to nerve agents are required for diagnosis, therapeutic monitoring, health surveillance and forensic purposes. Determination of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activity in blood remains a mainstay for the fast initial screening but lacks sensitivity and specificity. Quantitative analysis of nerve agents and their degradation products in plasma and urine by mass spectrometric methods may prove exposure but is limited to hours or days after the incident due to the short residence time of the analytes. Investigation of protein adducts extends the time interval between exposure and sampling and may be suitable to detect low-level exposure. Definitive prove of exposure requires a spectrum of different methods, expensive and sophisticated equipment and will be limited to specialized laboratories.
authors.affiliation: Bundeswehr Institute of Pharmacology and Toxicology, Neuherbergstrasse 11, 80937 Munich, Germany. franzworek@bundeswehr.org
authors.forename: Franz
authors.initials: F
authors.lastname: Worek
authors.forename: Marianne
authors.initials: M
authors.lastname: Koller
authors.forename: Horst
authors.initials: H
authors.lastname: Thiermann
authors.forename: Ladislaus
authors.initials: L
authors.lastname: Szinicz
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood*; D002091:Butyrylcholinesterase / Q000097:blood*; D006801:Humans; D062025:Organophosphate Poisoning; D010755:Organophosphates / Q000097:blood; D011041:Poisoning / Q000097:blood / Q000175:diagnosis
chemical_list: D010755:Organophosphates; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase