The transgenic Arabidopsis plant system, pER8-GFP, as a powerful tool in searching for natural product estrogen-agonists/antagonists.
The transgenic Arabidopsis plant system, pER8-GFP, may be used as a powerful tool in searching for natural estrogen-agonists/antagonists. Among selected plant extracts and natural products, the method was able to distinguish active extracts (e.g., Glycine max and Pueraria lobata) and pure compounds (e.g., 17beta-estradiol (1), genistein (10), and daidzein (11)) and also to distinguish effects of structural changes on activity. Thus, this rapid sensitive system was proven to be suitable for the discovery of natural products with estrogenic activity.
Chang
Fang-Rong
FR
Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan, Republic of China. aaronfrc@kmu.edu.tw
Hayashi
Ken-ichiro
K
Chua
Nam-Hai
NH
Kamio
Shuichi
S
Huang
Zih-You
ZY
Nozaki
Hiroshi
H
Wu
Yang-Chang
YC
D017360:Arabidopsis; D001685:Biological Factors / Q000302:isolation & purification* / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000302:isolation & purification*; D004967:Estrogens / Q000819:agonists* / Q000302:isolation & purification*; D048789:Phytoestrogens / Q000302:isolation & purification* / Q000494:pharmacology*; D030821:Plants, Genetically Modified; D013329:Structure-Activity Relationship
D001685:Biological Factors; D004965:Estrogen Antagonists; D004967:Estrogens; D048789:Phytoestrogens