[Daphnia magna Straus: a new model for evaluating the antioxidant acton of water-soluble preparations in vivo].
Hydrobionta species of Daphnia magna Straus were used as a test objects for in vivo evaluation of the antioxidant activity of three hydrophilic thiol compounds: reduced gluthatione, unithiol, and cysteine. These compounds exhibited significant differences in activity under oxidative stress conditions, in the dynamics of observed effects, and in the probability of inversion from anti- to pro-oxidant action. The main advantage of the proposed test objects in comparison to the conventional in vitro experiments (where the antioxidant effect is evaluated over a period of time from several minutes to several hours) is that the development of drug activity (pro- and antioxidant effects) can be monitored over a prolonged period of time (up to several days). In comparison to the tests on mammals, the new method is much simpler and allows the entire antioxidant protection (rather than separate systems) to be evaluated. It is recommended to use Daphnia magna Straus species for comparative evaluation of the antioxidant action of water-soluble preparations in vivo.
Podosinovikova
N P
NP
Petrov
V V
VV
Dolgo-Saburov
V B
VB
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D003545:Cysteine / Q000737:chemistry / Q000494:pharmacology; D003621:Daphnia / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D005978:Glutathione / Q000737:chemistry / Q000494:pharmacology; D008954:Models, Biological; D012996:Solutions / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000494:pharmacology*; D014494:Unithiol / Q000737:chemistry / Q000494:pharmacology; D014867:Water / Q000737:chemistry
D000975:Antioxidants; D012996:Solutions; D013438:Sulfhydryl Compounds; D014867:Water; D014494:Unithiol; D005978:Glutathione; D003545:Cysteine