Susceptibility to oxidative stress of mussels (Mytilus galloprovincialis) in the Venice Lagoon (Italy).
The aim of this study was to evaluate the susceptibility to pollutant mediated oxidative stress of the Mediterranean mussel Mytilus galloprovincialis in the Venice lagoon (Italy). In June 2003, mussels from a farm were transplanted to eight sites in the lagoon for five weeks. Oxidative stress responses were measured by: (i) total oxyradical scavenging capacity (TOSC) assay, for an overall evaluation of the oxidative stress response capability; (ii) catalase (CAT), as a key enzyme involved in the antioxidant defence system; (iii) malondialdehyde (MDA), as an indicator of lipid peroxidation, to evaluate an oxidative damage; (iv) metallothioneins (MTs), as they play a role in the antioxidant defence. The TOSC analysis revealed a reduced capability to eliminate: (i) peroxyl radical in mussels transplanted at Palude della Rosa, Valle Millecampi and Chioggia; (ii) hydroxyl radical at Campalto and Valle Millecampi; (iii) peroxynitrite at Valle Millecampi. Inhibition in CAT activity, observed in all the monitored sites, confirms the presence of an oxidative pressure in transplanted mussels. In addition, Pearson correlation analysis was performed in order to observe possible links between the various parameters. The PCA was a powerful tool to discriminate impacted sites, suggesting that the mussels transplanted throughout the Venice lagoon were subjected to different levels of oxidative pressure. Furthermore, it provided an easy and useful tool to summarize the obtained results.
Pampanin
Daniela M
DM
Institute of Marine Science, ISMAR-CNR, Castello 1363/a, Venice, Italy.
Camus
Lionel
L
Gomiero
Alessio
A
Marangon
Ilenia
I
Volpato
Elisa
E
Nasci
Cristina
C
D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D002374:Catalase / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods; D016166:Free Radical Scavengers / Q000737:chemistry; D006458:Hemolymph / Q000737:chemistry; D017665:Hydroxyl Radical / Q000032:analysis; D007558:Italy; D008315:Malondialdehyde / Q000032:analysis; D008668:Metallothionein / Q000032:analysis; D049878:Mytilus / Q000737:chemistry / Q000201:enzymology / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology*; D010545:Peroxides / Q000032:analysis; D030421:Peroxynitrous Acid / Q000032:analysis; D025341:Principal Component Analysis; D013223:Statistics as Topic
D015415:Biomarkers; D016166:Free Radical Scavengers; D010545:Peroxides; D030421:Peroxynitrous Acid; C049375:perhydroxyl radical; D017665:Hydroxyl Radical; D008315:Malondialdehyde; D008668:Metallothionein; D002374:Catalase