Therapeutic vaccination with papillomavirus E6 and E7 long peptides results in the control of both established virus-induced lesions and latently infected sites in a pre-clinical cottontail rabbit papillomavirus model.
This study was performed to test the therapeutic efficacy of overlapping long E6 and E7 peptides, containing both CD4+ T-helper and CD8+ CTL epitopes, on CRPV-induced lesions, which is an appropriate pre-clinical model for HPV diseases, including recurrent respiratory papillomatosis (RRP). Therapeutic peptide vaccination was able to significantly control wart growth (p < 0.01) and abrogate latent CRPV infection (p = 0.0006) compared to controls. Vaccination was associated with a T(H)1 T cell response, as suggested by a strong DTH skin test, antigen-specific proliferation of PBMC and a minimal IgG antibody response. Thus, this study shows promise for treatment of RRP by vaccination with long peptides.
Vambutas
A
A
North Shore-Long Island Jewish Research Institute, Long Island Jewish Medical Center, New Hyde Park, NY 11040, USA. vambutas@lij.edu
DeVoti
J
J
Nouri
M
M
Drijfhout
J W
JW
Lipford
G B
GB
Bonagura
V R
VR
van der Burg
S H
SH
Melief
C J M
CJ
D000818:Animals; D049109:Cell Proliferation; D012777:Cottontail rabbit papillomavirus / Q000276:immunology*; D004279:DNA, Viral / Q000032:analysis; D004847:Epithelial Cells / Q000276:immunology; D005805:Genes, MHC Class I / Q000276:immunology; D005802:Genes, MHC Class II / Q000235:genetics; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular / Q000276:immunology; D007150:Immunohistochemistry; D009000:Monocytes / Q000276:immunology; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D011817:Rabbits; D012882:Skin Tests; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014412:Tumor Virus Infections / Q000276:immunology / Q000628:therapy* / Q000821:virology; D014611:Vaccination; D017735:Virus Latency; D014860:Warts / Q000276:immunology / Q000473:pathology / Q000517:prevention & control
D004279:DNA, Viral; C469554:E6 protein, Cottontail rabbit papillomavirus; C079183:E7 protein, cottontail rabbit papillomavirus; D009856:Oncogene Proteins, Viral