title: Extracellular oxidases of the lignin-degrading fungus Panus tigrinus.
abstract: Two extracellular oxidases (laccases) were isolated from the extracellular fluid of the fungus Panus (Lentinus) tigrinus cultivated in low-nitrogen medium supplemented with birch sawdust. The enzymes were purified by successive chromatography on columns with TEAE-cellulose and DEAE-Toyopearl 650M. Both oxidases catalyze oxidation of pyrocatechol and ABTS. Moreover, oxidase 1 also catalyzes oxidation of guaiacol, o-phenylenediamine, and syringaldazine. The enzymes have identical pH (7.0) and temperature (60-65 degrees C) optimums. Absorption spectra of the oxidases differ from the spectra of typical "blue" laccases and are similar to the spectrum of yellow oxidase.
authors.affiliation: Department of Biotechnology, Biological Faculty, Ogarev Mordovian State University, Bolshevistskaya ul. 68, 430000 Saransk, Russia. biotech@moris.ru
authors.forename: D A
authors.initials: DA
authors.lastname: Cadimaliev
authors.forename: V V
authors.initials: VV
authors.lastname: Revin
authors.forename: N A
authors.initials: NA
authors.lastname: Atykyan
authors.forename: V D
authors.initials: VD
authors.lastname: Samuilov
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D002384:Catalysis; D002852:Chromatography, Ion Exchange; D042845:Laccase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D010084:Oxidation-Reduction; D013379:Substrate Specificity
chemical_list: D008031:Lignin; D042845:Laccase