[Effect of hypoxia on the expression of urokinase-type plasminogen activator in human tongue squamous cell carcinoma cells (Tca8113)].
OBJECTIVE
To observe the expression of urokinase-type plasminogen activator (uPA) in human tongue squamous carcinoma cells (Tca8113) under hypoxia, in order to explore the relation between hypoxia and invasion and metastasis of oral cancer.


METHODS
Under different hypoxic times(0, 3, 6, 12, 24 h), the expression of uPA protein was examined quantitatively using immunohistochemical technique (IH) and flow cytometry (FCM).


RESULTS
Hypoxia promoted the expression of uPA. The longer hypoxic time was, the higher expression of uPA was.


CONCLUSIONS
Hypoxia can promote invasion and metastasis of oral squamous carcinoma through stimulating the expression of uPA.
Wang
Sheng-Zhi
SZ
Dept. of Oral and Maxillofacial Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai 264000, China.
Liang
Xin-Hua
XH
Cheng
Zhou
Z
Bi
Hong-Guang
HG
Mao
Zu-Yi
ZY
Wang
Do-Zhang
DZ
D002294:Carcinoma, Squamous Cell; D006801:Humans; D009062:Mouth Neoplasms; D009361:Neoplasm Invasiveness; D014062:Tongue Neoplasms; D014568:Urokinase-Type Plasminogen Activator
D014568:Urokinase-Type Plasminogen Activator