High-yield solution-phase synthesis of di- and trinucleotide blocks assisted by polymer-supported reagents.
A new solution-phase phosphoramidite approach is reported for oligonucleotide synthesis employing recyclable solid-supported reagents. It uses polyvinyl pyridinium tosylate as the activator of a nucleoside-3'-O-phosphoramidite in the coupling step with a 5'-OH nucleoside or dinucleotide. The resulting phosphite triester was either sulfurized or oxidized using polystyrene-bound trimethylammonium tetrathionate or periodiate. This method avoids complicated purification steps, as excess reagents are easily removed by filtration. [reaction: see text]
Dueymes
Cécile
C
ERT Oligonucléotides: Méthodologie, Valorisation, UMR 5625 CNRS-UM2 University Montpellier II, 34095 Montpellier, France.
Schönberger
Andreas
A
Adamo
Ilaria
I
Navarro
Aude-Emmanuelle
AE
Meyer
Albert
A
Lange
Meinolf
M
Imbach
Jean-Louis
JL
Link
Fritz
F
Morvan
François
F
Vasseur
Jean-Jacques
JJ
D002384:Catalysis; D007202:Indicators and Reagents; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*
D007202:Indicators and Reagents; D009841:Oligonucleotides; D011108:Polymers