title: The large first periplasmic loop of SecD and SecF plays an important role in SecDF functioning.
abstract: A remarkable feature of proteins of the SecD and SecF family involved in protein translocation is that they possess a very large first periplasmic domain. Here we report that this large first periplasmic domain is not required for the SecD-SecF interaction but that it is important for catalyzing protein translocation.
authors.affiliation: Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands. n.p.nouwen@rug.nl
authors.forename: Nico
authors.initials: N
authors.lastname: Nouwen
authors.forename: Magdalena
authors.initials: M
authors.lastname: Piwowarek
authors.forename: Greetje
authors.initials: G
authors.lastname: Berrelkamp
authors.forename: Arnold J M
authors.initials: AJ
authors.lastname: Driessen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016296:Mutagenesis; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C499394:SecD protein, E coli; C489583:SecF protein, E coli; C075767:secD protein, Bacteria