Structural instability caused by a mutation at a conserved arginine in the alpha-crystallin domain of Chinese hamster heat shock protein 27.
Mutations in the alpha-crystallin domain of 4 of the small heat shock proteins (sHsp) (Hsp27/HspB1, alphaA-crystallin/ HspB4, alphaB-crystallin/HspB5, and HspB8) are responsible for dominant inherited diseases in humans. One such mutation at a highly conserved arginine residue was shown to cause major conformational defects and intracellular aggregation of alphaA- and alphaB-crystallins and HspB8. Here, we studied the effect of this Arg mutation on the structure and function of Hsp27. Chinese hamster Hsp27 with Arg148 replaced by Gly (Hsp27R148G) formed dimers in vitro and in vivo, which contrasted with the 12- or 24-subunit oligomers formed by the wild-type protein (Hsp27WT). Despite these alterations, Hsp27R148G had a chaperone activity almost as high as Hsp27WT. The dimers of Hsp27R148G did not further deoligomerize on phosphorylation and like the dimers formed by phosphorylated Hsp27WT were not affected by the deletion of the N-terminal WD/EPF (single letter amino acid code) motif, suggesting that mutation of Arg148, deletion of the N-terminal WD/EPF motif, and phosphorylation of Ser90 may produce similar structural perturbations. Nevertheless, the structure of Hsp27R148G appeared unstable, and the mutated protein accumulated as aggregates in many cells. Both a lower basal level of phosphorylation of Hsp27R148G and the coexpression of Hsp27WT could reduce the frequency of formation of these aggregates, suggesting possible mechanisms regulating the onset of the sHsp-mediated inherited diseases.
Chávez Zobel
Aura T
AT
Departamento de Morfología, Universidad Centroccidental Lisandro Alvarado, Decanato de Medicina, Sección de Anatomía Microscópica, Avenida Libertador con Avenida Andrés Bello, Barquisimeto, Estado Lara, Venezuela.
Lambert
Herman
H
Thériault
Jimmy R
JR
Landry
Jacques
J
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001120:Arginine / Q000737:chemistry / Q000235:genetics*; D017124:Conserved Sequence; D006224:Cricetinae; D003412:Cricetulus; D005455:Fluorescent Antibody Technique; D005976:Glutaral; D005990:Glycerol; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D002479:Inclusion Bodies / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics*; D017354:Point Mutation; D013997:Time Factors
D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D001120:Arginine; D005990:Glycerol; D005976:Glutaral