STAT4 is a target of the hematopoietic zinc-finger transcription factor Ikaros in T cells.
STAT4 is a transcription factor activated in response to IL-12, and is involved in Th1 cell development. The molecular mechanisms controlling the transcription of the STAT4 gene are however, unclear. Sequence comparison of the 5' flanking regions of human, mouse and pufferfish (Fugu rubripes) Stat4 genes revealed a high frequency of Ikaros (Ik) binding elements in all three species. We then investigated the role of Ik binding elements in the human STAT4 promoter using Jurkat T cells. Transactivation, electrophoretic mobility shift assay and RNA interference-mediated gene knockdown experiments revealed that Ik is involved in the regulation of STAT4 in human T cells.
Yap
Wai-Ho
WH
Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673, Singapore.
Yeoh
Elizabeth
E
Tay
Alice
A
Brenner
Sydney
S
Venkatesh
Byrappa
B
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D016678:Genome; D006410:Hematopoiesis / Q000235:genetics; D006801:Humans; D051740:Ikaros Transcription Factor; D019169:Jurkat Cells; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D050797:STAT4 Transcription Factor; D013601:T-Lymphocytes / Q000378:metabolism*; D023741:Takifugu / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000235:genetics*; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; C497239:IKZF1 protein, human; D050797:STAT4 Transcription Factor; C494090:STAT4 protein, human; C494091:Stat4 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; C497238:Zfpn1a1 protein, mouse; D051740:Ikaros Transcription Factor