The HIV-1 capsid protein C-terminal domain in complex with a virus assembly inhibitor.
Immature HIV particles bud from infected cells after assembly at the cytoplasmic side of cellular membranes. This assembly is driven by interactions between Gag polyproteins. Mature particles, each containing a characteristic conical core, are later generated by proteolytic maturation of Gag in the virion. The C-terminal domain of the HIV-1 capsid protein (C-CA) has been shown to contain oligomerization determinants essential for particle assembly. Here we report the 1.7-A-resolution crystal structure of C-CA in complex with a peptide capable of inhibiting immature- and mature-like particle assembly in vitro. The peptide inserts as an amphipathic alpha-helix into a conserved hydrophobic groove of C-CA, resulting in formation of a compact five-helix bundle with altered dimeric interactions. This structure thus reveals the details of an allosteric site in the HIV capsid protein that can be targeted for antiviral therapy.
Ternois
François
F
Laboratoire de Virologie Moléculaire & Structurale, UMR 2472/1157 CNRS-INRA and IFR 115, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
Sticht
Jana
J
Duquerroy
Stéphane
S
Kräusslich
Hans-Georg
HG
Rey
Félix A
FA
D036022:Capsid Proteins / Q000737:chemistry / Q000378:metabolism*; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism / Q000502:physiology*; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D019065:Virus Assembly / Q000235:genetics* / Q000502:physiology; D014961:X-Ray Diffraction
D036022:Capsid Proteins; D015683:Gene Products, gag; D046912:Multiprotein Complexes; D010455:Peptides