title: [Altered expression of the HSD17B4 gene in esophageal squamous cell carcinoma and loss of heterozygosity analysis].
abstract: OBJECTIVE
  To investigate the alteration of the gene HSD17B4 in esophageal squamous cell carcinoma and its potential significance.
  
  
  METHODS
  The mRNA expression and loss of heterozygosity (LOH) of HSD17B4 in 40 primary esophageal tumors were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and microsatellite analysis with the intragenic marker D5S1384 of the gene.
  
  
  RESULTS
  The frequencies of allelic loss of D5S1384 and the rate of down-regulation of gene HSD17B4 were 46.2% and 62.5%, respectively.
  
  
  CONCLUSIONS
  HSD17B4 may be a candidate tumor suppressor gene associated with esophageal squamous cell carcinoma.
authors.affiliation: State Key Laboratory of Molecular Oncology, Cancer Institute, CAMS and PUMC, Beijing 100021, China.
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Li
authors.forename: Xiang-yang
authors.initials: XY
authors.lastname: Liu
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Huang
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Fu
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Cai
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Han
authors.forename: Tie-hua
authors.initials: TH
authors.lastname: Rong
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.forename: Qi-min
authors.initials: QM
authors.lastname: Zhan
authors.forename: Ming-rong
authors.initials: MR
authors.lastname: Wang
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000096:biosynthesis / Q000235:genetics*; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D015536:Down-Regulation; D004746:Enoyl-CoA Hydratase / Q000096:biosynthesis / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006801:Humans; D006836:Hydro-Lyases; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000235:genetics*; D064029:Peroxisomal Multifunctional Protein-2; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D009097:Multienzyme Complexes; D012333:RNA, Messenger; D015067:17-Hydroxysteroid Dehydrogenases; D006836:Hydro-Lyases; D064029:Peroxisomal Multifunctional Protein-2; C104587:HSD17B4 protein, human; D004746:Enoyl-CoA Hydratase