title: Two-dimensional gel analysis of protein expression profile in squamous cervical cancer patients.
abstract: OBJECTIVE
  Screening in cervical cancer is progressing to find out candidate genes and proteins, which may work as biological markers and play a role in tumor progression. We examined the protein expression patterns of squamous cell carcinoma (SCC) tissues from Korean women using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometer.
  
  
  METHODS
  Normal cervix and SCC tissues were solubilized and 2-DE was performed using the pH 3-10 linear IPG strips of 17 cm length and silver stained. Protein expression was evaluated using PDQuest 2-D software. The differentially expressed protein spots were identified with MALDI-TOF mass spectrometer and the peptide mass spectra identification was performed using Mascot program searching the Swiss-prot or NCBInr databases.
  
  
  RESULTS
  A total of 35 proteins were detected in SCC. 17 proteins were up-regulated and 18 proteins were down-regulated. Among the proteins identified, 12 proteins (pigment epithelium derived factor, annexin A2 and A5, keratin 19 and 20, heat shock protein 27, smooth muscle protein 22 alpha, alpha-enolase, squamous cell carcinoma antigen 1 and 2, glutathione S-transferase, apolipoprotein a1) were previously known proteins involved in tumor and 21 proteins were newly identified in this study.
  
  
  CONCLUSIONS
  2-DE offers total protein expression profiles of SCC tissues and further characterization of proteins that are differentially expressed will give a chance to identify tumor-specific diagnostic markers for SCC.
authors.affiliation: Cancer Research Center, Catholic Research Institutes of Medical Science, The Catholic University of Korea, Seoul 137-040, South Korea.
authors.forename: Su Mi
authors.initials: SM
authors.lastname: Bae
authors.forename: Chang-Hun
authors.initials: CH
authors.lastname: Lee
authors.forename: Young Lae
authors.initials: YL
authors.lastname: Cho
authors.forename: Kye Hyun
authors.initials: KH
authors.lastname: Nam
authors.forename: Yong Wan
authors.initials: YW
authors.lastname: Kim
authors.forename: Chong Kook
authors.initials: CK
authors.lastname: Kim
authors.forename: Byoung Don
authors.initials: BD
authors.lastname: Han
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Lee
authors.forename: Heung Jae
authors.initials: HJ
authors.lastname: Chun
authors.forename: Woong Shick
authors.initials: WS
authors.lastname: Ahn
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D014176:Protein Biosynthesis; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014335:Tropomyosin / Q000032:analysis / Q000235:genetics; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: D009363:Neoplasm Proteins; D014335:Tropomyosin