title: Analysis of properdin (BF) genotypes associated with litter size in a commercial pig cross population.
abstract: Properdin (BF) was investigated as a candidate gene influencing litter size in a commercial pig cross population. The BF gene was chosen because of its integral role in influencing uterine epithelium growth and because several quantitative trait loci (QTL) with impact on reproductive traits have been detected near the centromere of porcine chromosome 7. A total of 123 F2 (Large White x Landrace) x Leicoma sows were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The sows were divided into two extreme performance groups, one with a high litter size (n = 61, > or = 14.3 piglets per litter) and the other with a low litter size (n = 62, < or = 11.3 piglets per litter). Although genotype and allele frequencies were uneven with 2.4% (AA), 16.3% (AB), 81.3% (BB) and 0.11 (A): 0.89 (B), the allele A was the unfavourable one, leading to less offspring. With regard to the level of significance at p < 0.05, the total number of born (TNB) and number of born alive (NBA) piglets were associated with BF genotypes. The genotype AA led to 10.55 TNB and 10.00 NBA, whereas the genotype BB led to 13.19 TNB and 12.11 NBA. The genotype AB was intermediate. In future, a systematic mating test is necessary in order to obtain more balanced genotype frequencies. Furthermore, it should be taken into consideration that the investigated polymorphism is located in an intronic region and the causative mutation is not clear yet.
authors.affiliation: Department of Breeding Biology and Molecular Genetics, Institute of Animal Sciences, Agricultural and Horticultural Faculty, Humboldt-University of Berlin, Berlin, Germany. bernd.buske@staff.hu-berlin.de
authors.forename: B
authors.initials: B
authors.lastname: Buske
authors.forename: C
authors.initials: C
authors.lastname: Brunsch
authors.forename: K
authors.initials: K
authors.lastname: Zeller
authors.forename: P
authors.initials: P
authors.lastname: Reinecke
authors.forename: G
authors.initials: G
authors.lastname: Brockmann
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D008098:Litter Size / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D011414:Properdin / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics; D013552:Swine / Q000235:genetics / Q000502:physiology*
chemical_list: D011414:Properdin