Resistance to eyespot of wheat, caused by Tapesia yallundae, derived from Thinopyrum intermedium homoeologous group 4 chromosome.
Thinopyrum intermedium was identified previously as resistant to Tapesia yallundae, cause of eyespot of wheat. Using GUS-transformed isolates of T. yallundae as inoculum, we determined that wheat lines carrying Th. intermedium chromosome 4 Ai#2 or the short arm of chromosome 4 Ai#2 were as resistant to the pathogen as the eyespot-resistant wheat- Th. ponticum chromosome substitution line SS 767 (PI 611939) and winter wheat cultivar Madsen, which carries gene Pch 1 for eyespot resistance. Chromosome 4 E from Th. elongatum and chromosome 4 J from Th. bessarabicum did not confer resistance to T. yallundae. Genome-specific PCR primers confirmed the presence of Thinopyrum chromatin in these wheat- Thinopyrum lines. Genomic in situ hybridization using an St genomic probe from Pseudoroegneria strigosa demonstrated that chromosome 4 Ai#2 belongs to the J(s) genome of Thinopyrum. The eyespot resistance in the wheat- Th. intermedium lines is thus controlled by the short arm of this J(s) chromosome. This is the first report of resistance to T. yallundae controlled by a J(s) genome chromosome of Th. intermedium.
Li
H J
HJ
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China.
Arterburn
M
M
Jones
S S
SS
Murray
T D
TD
D001203:Ascomycota / Q000235:genetics* / Q000472:pathogenicity*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018745:Genome, Plant; D007113:Immunity, Innate / Q000235:genetics; D017403:In Situ Hybridization; D010935:Plant Diseases / Q000382:microbiology; D014908:Triticum / Q000235:genetics* / Q000382:microbiology