Inhibition by adiponectin of IL-8 production by human macrophages upon coculturing with late apoptotic cells.
Adiponectin, an adipocyte-derived hormone, reportedly suppresses the production of TNF-alpha and IL-6 by LPS-stimulated human or porcine macrophages, and the phagocytosis of microbeads by human macrophages. In this study, we used a high molecular weight form of adiponectin purified from human plasma to examine its effects on the phagocytosis of late apoptotic cells by human macrophages and the subsequent IL-8 production. Adiponectin suppressed both the phagocytosis of apoptotic cells and the IL-8 production. In contrast, adiponectin augmented both the phagocytosis of apoptotic cells and the IL-8 production in the presence of LPS. These results suggest that adiponectin is not an anti-inflammatory hormone but rather a dual modulator of innate responses.
Saijo
Shinji
S
Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1, Miyama, Funabashi, Chiba, Japan.
Nagata
Kisaburo
K
Nakano
Yasuko
Y
Tobe
Takashi
T
Kobayashi
Yoshiro
Y
D052242:Adiponectin; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D016209:Interleukin-8 / Q000096:biosynthesis*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*
D052242:Adiponectin; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8