Overexpression of QM induces cell differentiation and mineralization in MC3T3-E1.
It has been reported that QM was highly expressed by cells isolated from epiphyseal cartilage as opposed to proliferative chondrocytes. In vitro investigation of the expression of QM revealed higher QM expression in nonmineralizing osteoblast and pericyte cultures as compared with mineralizing cultures. These evidences suggest that QM may play an essential role in cell differentiation before mineralization. However, our research results showed that QM overexpression in MC3T3-E1 enhanced cell differentiation and mineralization. In this study, alkaline phosphatase (ALP) activity and nodule mineralization were increased in MC3T3-E1 from QM overexpression cultures relative to normal expression QM cultures. RT-PCR revealed upregulation of the marker genes type I collagen, ALP, osteocalcin, osterix and BMP-2 and a slight decrease of a negative regulator osteopontin. These results suggest that the increasing of QM expression could stimulate osteoblast differentiation and mineralization in MC3T3-E1.
Zhao
Ying
Y
Department of Biological Sciences and Biotechnology, Medical School, Tsinghua University, Beijing 100084, P.R. China.
Guan
Hong
H
Liu
Shang-Feng
SF
Wu
Rong-Cong
RC
Wang
Zhao
Z
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002113:Calcification, Physiologic; D002454:Cell Differentiation / Q000235:genetics*; D024042:Collagen Type I / Q000378:metabolism; D017931:DNA Primers; D051379:Mice; D015675:Osteocalcin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000080882:Ribosomal Protein L10; D012269:Ribosomal Proteins / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics*
C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I; D017931:DNA Primers; D012269:Ribosomal Proteins; C000630690:Rpl10 protein, mouse; D016212:Transforming Growth Factor beta; D025521:Tumor Suppressor Proteins; D015675:Osteocalcin; D000469:Alkaline Phosphatase