Assessment of paraoxonase 1 activity and malondialdehyde levels in patients with rheumatoid arthritis.
OBJECTIVE
We aimed to evaluate antioxidant paraoxonase 1 activity together with malondialdehyde (MDA) (an oxidative stress parameter) levels in patients with rheumatoid arthritis.


METHODS
Fifty-seven rheumatoid arthritis patients were included in the study and subgrouped according to disease activity (active, n = 31; inactive, n = 26) and compared with healthy controls (n = 25). Serum paraoxonase 1 activity and MDA levels were measured according to an enzymatic spectrophotometric method.


RESULTS
Serum MDA level was higher (P = 0.001) whereas paraoxonase 1 activity was lower (P = 0.001) in the patient group than the controls. When active and inactive subgroups were compared with the control group, there was a statistically significant difference between each parameter. Serum MDA levels were significantly higher, while paraoxonase 1 activity was lower in the active and inactive rheumatoid arthritis groups than the control group. But there was not any difference between active and inactive patients with RA. There was a negative correlation between MDA levels and paraoxonase 1 activity.


CONCLUSIONS
Increased reactive oxygen species levels in rheumatoid arthritis may result in a pro-oxidation environment, which in turn could result in decreased antioxidant paraoxonase 1 activity and increased MDA levels.
Baskol
Gulden
G
Department of Biochemistry and Clinical Biochemistry, Erciyes University Faculty of Medicine, Kayseri, Turkey. gbaskol@yahoo.com
Demir
Huseyin
H
Baskol
Mevlut
M
Kilic
Eser
E
Ates
Filiz
F
Kocer
Derya
D
Muhtaroglu
Sabahattin
S
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood*; D043303:Aryldialkylphosphatase / Q000097:blood*; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D017382:Reactive Oxygen Species / Q000097:blood
D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D043303:Aryldialkylphosphatase