title: High-yield solution-phase synthesis of di- and trinucleotide blocks assisted by polymer-supported reagents.
abstract: A new solution-phase phosphoramidite approach is reported for oligonucleotide synthesis employing recyclable solid-supported reagents. It uses polyvinyl pyridinium tosylate as the activator of a nucleoside-3'-O-phosphoramidite in the coupling step with a 5'-OH nucleoside or dinucleotide. The resulting phosphite triester was either sulfurized or oxidized using polystyrene-bound trimethylammonium tetrathionate or periodiate. This method avoids complicated purification steps, as excess reagents are easily removed by filtration. [reaction: see text]
authors.affiliation: ERT Oligonucléotides: Méthodologie, Valorisation, UMR 5625 CNRS-UM2 University Montpellier II, 34095 Montpellier, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Dueymes
authors.forename: Andreas
authors.initials: A
authors.lastname: Schönberger
authors.forename: Ilaria
authors.initials: I
authors.lastname: Adamo
authors.forename: Aude-Emmanuelle
authors.initials: AE
authors.lastname: Navarro
authors.forename: Albert
authors.initials: A
authors.lastname: Meyer
authors.forename: Meinolf
authors.initials: M
authors.lastname: Lange
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Imbach
authors.forename: Fritz
authors.initials: F
authors.lastname: Link
authors.forename: François
authors.initials: F
authors.lastname: Morvan
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Vasseur
mesh_terms: D002384:Catalysis; D007202:Indicators and Reagents; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D009841:Oligonucleotides; D011108:Polymers