Temperature alternation by an on-chip microheater to reveal enzymatic activity of beta-galactosidase at high temperatures.
A method to measure enzymatic activity at high temperatures by rapid temperature alternation of a microreactor with a microheater is proposed. On-chip microreactor and microheater were integrated on a glass plate by MEMS technology; this microheater can control the temperature of the microreactor with a response speed of 34.2 and 31.5 K/s for temperature rise and fall, respectively, with an accuracy of 3 degrees C. The enzyme, beta-galactosidase, was revealed to survive short exposure (4-s pulses) to temperatures above that which would "normally" denature them. Its activity at 60 degrees C was revealed to be approximately 4 times greater than that at room temperature. This method not only gives new kinetic information in biochemistry but also enables application in highly sensitive biosensors.
Arata
Hideyuki F
HF
Institute of Industrial Science, The University of Tokyo, Meguro-ku, Tokyo 153-8505, Japan. hideyuki@fujita3.iis.u-tokyo.ac.jp
Rondelez
Yannick
Y
Noji
Hiroyuki
H
Fujita
Hiroyuki
H
D006358:Hot Temperature; D056656:Lab-On-A-Chip Devices; D046208:Microchip Analytical Procedures / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D001616:beta-Galactosidase / Q000378:metabolism* / Q000648:ultrastructure
D001616:beta-Galactosidase