Activation of NF-kappaB in bone marrow cells of BALB/cJ mice following exposure in vivo to low doses of (137)Cs gamma-rays.
We measured levels of NF-kappaB activation in bone marrow (BM) cells collected at 1 and 4 h from male BALB/cJ mice (10-12 weeks old) given a whole body dose of 0, 0.05, 0.1 and 1 Gy of (137)Cs gamma-rays (at the dose rate of 0.75 Gy/min). At each harvest time-point, BM cells were collected from five mice per dose of radiation. We used two methods for detecting NF-kappaB activation (1) the NF-kappaB/p65 transcription factor enzyme-linked immunosorbance assay (ELISA) and (2) immunofluorescence staining with NF-kappaB/p65 antibody. Results from ELISA indicated 2.0 and 2.8-fold increases in NF-kappaB activation in BM cells isolated at 1 h post-exposure of mice to 0.1 or 1.0 Gy. The immunofluorescence staining method showed similar results. In samples isolated 4 h post-irradiation, however, no activated NF-kappaB signal was found, regardless of the method of detection. The data also demonstrated that NF-kappaB was not activated in bone marrow cells collected either at 1 or 4 h from BALB/cJ mice exposed to a single dose of 0.05 Gy (137)Cs gamma-rays. Taken together, the results from our in vivo study indicate the involvement of NF-kappaB activation in early response to 0.1 and 1.0 Gy (but not 0.05 Gy) of (137)Cs gamma-rays.
Rithidech
Kanokporn Noy
KN
Pathology Department, State University of New York at Stony Brook, Stony Brook, NY 11794-8691, USA. krithidech@notes.cc.sunysb.edu
Tungjai
Montree
M
Arbab
Edgar
E
Simon
Sanford R
SR
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000528:radiation effects*; D002588:Cesium Radioisotopes / Q000009:adverse effects; D004307:Dose-Response Relationship, Radiation; D004797:Enzyme-Linked Immunosorbent Assay; D005720:Gamma Rays / Q000009:adverse effects*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051996:Transcription Factor RelA / Q000378:metabolism*
D002588:Cesium Radioisotopes; D051996:Transcription Factor RelA