[Effects of astragalus polysaccharides-chitosan/polylactic acid composite material on biological behavior of canine bone marrow stromal cells cultured in vitro].
OBJECTIVE
To observe the biological behavior of canine bone marrow stromal cells (BMSCs) cultured in vitro with the astragalus polysaccharides-chitosan/polylactic acid (AP-C/PLA) and with the chitosan/polylactic acid (C/PLA) and to find a suitable compound material for periodontal tissue engineering.


METHODS
BMSCs (induced 14 days by 50 mg/L vitamine C, 10(-8) mol/L dexamethasone, 10 mmol/L beta-sodium glycerylphosphate) were cultured on AP-C/PLA or C/PLA for 5 days respectively. The BMSCs attachment and the morphology were observed with scanning electronic microscope and the combining rates were counted. Type I collagen synthesis was examined with immunohistochemistry staining and the content of osteocalin was determined with radio-immunological method.


RESULTS
Combining rates, type I collagen synthesis, and the content of osteocalin of BMSCs on AP-C/PLA were significantly higher than those on C/PLA.


CONCLUSIONS
AP-C/PLA may promote the BMSC proliferation, differentiation and extracellular matrix synthesis, and it can be used as a good scaffold material for bone tissue engineering.
Xu
Chun-Jiao
CJ
Department of Periodontology; Xiangya Hospital, Central South University, Changsha, China. jianxinchun@hotmail.com
Jian
Xin-Chun
XC
Peng
Jie-Ying
JY
Guo
Feng
F
Huang
Bai-Ying
BY
Xiong
Cheng-Dong
CD
Pan
Gao-Feng
GF
D000818:Animals; D027885:Astragalus propinquus; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D024042:Collagen Type I / Q000096:biosynthesis; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D019344:Lactic Acid / Q000494:pharmacology*; D008297:Male; D015675:Osteocalcin / Q000096:biosynthesis; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology*; D023822:Tissue Engineering
D024042:Collagen Type I; D004365:Drugs, Chinese Herbal; D011091:Polyesters; D011108:Polymers; D011134:Polysaccharides; D015675:Osteocalcin; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan