Identification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico.
Three isolates of Histoplasma capsulatum were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel's ornamental potted plants that had been fertilized with organic material known as compost. The presence of H. capsulatum in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual histoplasmosis outbreak in Acapulco. Although, diversity between the H. capsulatum isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of H-anti and ole fragment genes revealed a high homology (92-99%) between them.
Taylor
Maria Lucia
ML
Laboratorio de Inmunología de Hongos, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma México (UNAM), Ciudad Universitaria s/n. emello@servidor.unam.mx
Ruíz-Palacios
Guillermo M
GM
del Rocío Reyes-Montes
María
M
Rodríguez-Arellanes
Gabriela
G
Carreto-Binaghi
Laura E
LE
Duarte-Escalante
Esperanza
E
Hernández-Ramírez
Aurora
A
Pérez
Armando
A
Suárez-Alvarez
Roberto O
RO
Roldán-Aragón
Yuri A
YA
Romero-Martínez
Rafael
R
Sahaza-Cardona
Jorge H
JH
Sifuentes-Osornio
José
J
Soto-Ramírez
Luis E
LE
Peña-Sandoval
Gabriela R
GR
D000818:Animals; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005656:Fungal Proteins / Q000235:genetics; D006658:Histoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006660:Histoplasmosis / Q000453:epidemiology* / Q000382:microbiology / Q000473:pathology; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014195:Travel
D005656:Fungal Proteins