title: Identification of genes expressed in response to acid stress in Synechocystis sp. PCC 6803 using DNA microarrays.
abstract: Plant cells are always exposed to various environmental stresses such as high light, low temperature and acid rain, and thus have to respond in order to survive these stresses. Although some mechanisms of responses to high light and low temperature etc., have been clarified, there is little information about the acclimation process to acid stress. In this study, the gene expression changes of Synechocystis sp. PCC 6803 in response to acid stress were examined using DNA microarrays (CyanoCHIP). We compared gene expression profiles of the cells treated at pH 8 (control) and pH 3 for 0.5, 1, 2 or 4 h. As a result, we found that 32 genes were upregulated by more than 3-fold, and 29 genes were downregulated by at least 3-fold after the acid treatment. Among these upregulated genes, expressions of slr0967 and sll0939 kept-increasing until 4 h under the acid stress and increased by 7 to 16-fold after the 4 h treatment. This suggests that the products of these two genes play important roles in the acid acclimation process.
authors.affiliation: Department of Biology, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Tokyo 162-8601, Japan.
authors.forename: Hisataka
authors.initials: H
authors.lastname: Ohta
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Shibata
authors.forename: Youhei
authors.initials: Y
authors.lastname: Haseyama
authors.forename: Yuka
authors.initials: Y
authors.lastname: Yoshino
authors.forename: Takehiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kagasawa
authors.forename: Ayako
authors.initials: A
authors.lastname: Kamei
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikeuchi
authors.forename: Isao
authors.initials: I
authors.lastname: Enami
mesh_terms: D000222:Adaptation, Physiological; D015536:Down-Regulation / Q000187:drug effects; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects* / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D020411:Oligonucleotide Array Sequence Analysis; D046939:Synechocystis / Q000187:drug effects* / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects