title: Study of the interaction of proteins with curcumin and SDS and its analytical application.
abstract: It is found that protein and sodium dodecyl sulphonate (SDS) can enhance resonance light scattering (RLS) of curcumin (CU). Based on this phenomenon, a new quantitative method for protein in aqueous solution has been developed. In the BR (pH 3.5) buffer, the RLS intensity of CU-SDS system is greatly enhanced by protein. The enhanced RLS is proportional to the concentration of protein in the range of 0.00020-20.0 microgml(-1) for bovine serum albumin (BSA) and 0.00040-1.0 microgml(-1) for human serum albumin (HSA) and their detection limits are 0.16 and 0.041 ngml(-1), respectively. An actual sample is satisfactorily determined. In addition, the interaction mechanism between protein and CU-SDS is also studied by using multi-techniques such as RLS, absorption spectroscopy and fluorescence, zeta potential assay measurement.
authors.affiliation: Key Lab of Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Jinghe
authors.initials: J
authors.lastname: Yang
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Shufang
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D002021:Buffers; D002417:Cattle; D003474:Curcumin / Q000737:chemistry* / Q000528:radiation effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D002021:Buffers; D012709:Serum Albumin; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D003474:Curcumin