Identification of a crucial site for synoviolin expression.
Synoviolin is an E3 ubiquitin ligase localized in the endoplasmic reticulum (ER) and serving as ER-associated degradation system. Analysis of transgenic mice suggested that synoviolin gene dosage is implicated in the pathogenesis of arthropathy. Complete deficiency of synoviolin is fatal embryonically. Thus, alternation of Synoviolin could cause breakdown of ER homeostasis and consequently lead to disturbance of cellular homeostasis. Hence, the expression level of Synoviolin appears to be important for its biological role in cellular homeostasis under physiological and pathological conditions. To examine the control of protein level, we performed promoter analysis to determine transcriptional regulation. Here we characterize the role of synoviolin transcription in cellular homeostasis. The Ets binding site (EBS), termed EBS-1, from position -76 to -69 of the proximal promoter, is responsible for synoviolin expression in vivo and in vitro. Interestingly, transfer of EBS-1 decoy into NIH 3T3 cells conferred not only the repression of synoviolin gene expression but also a decrease in cell number. Fluorescence-activated cell sorter analysis using annexin V staining confirmed the induction of apoptosis by EBS-1 decoy and demonstrated recovery of apoptosis by overexpression of Synoviolin. Our results suggest that transcriptional regulation of synoviolin via EBS-1 plays an important role in cellular homeostasis. Our study provides novel insight into the transcriptional regulation for cellular homeostasis.
Tsuchimochi
Kaneyuki
K
Department of Genomic Science, Institute of Medical Science, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan.
Yagishita
Naoko
N
Yamasaki
Satoshi
S
Amano
Tetsuya
T
Kato
Yukihiro
Y
Kawahara
Ko-ichi
K
Aratani
Satoko
S
Fujita
Hidetoshi
H
Ji
Fengyun
F
Sugiura
Akiko
A
Izumi
Toshihiko
T
Sugamiya
Asako
A
Maruyama
Ikuro
I
Fukamizu
Akiyoshi
A
Komiya
Setsuro
S
Nishioka
Kusuki
K
Nakajima
Toshihiro
T
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000737:chemistry; D017209:Apoptosis; D001169:Arthritis, Experimental; D001483:Base Sequence; D001665:Binding Sites; D015153:Blotting, Western; D002469:Cell Separation; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000737:chemistry; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D008070:Lipopolysaccharides / Q000737:chemistry; D051379:Mice; D008822:Mice, Transgenic; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D041681:NIH 3T3 Cells; D009841:Oligonucleotides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets; D034741:RNA, Small Interfering / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D019076:Transgenes; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D001616:beta-Galactosidase / Q000378:metabolism
D017304:Annexin A5; D000911:Antibodies, Monoclonal; D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D009841:Oligonucleotides; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D034741:RNA, Small Interfering; D014157:Transcription Factors; D004247:DNA; C498235:Syvn1 protein, mouse; D044767:Ubiquitin-Protein Ligases; D001616:beta-Galactosidase