Longitudinal assessment of HIV-1 and HSV-2 shedding in the genital tract of West African women.
Forty-two HSV-2-seropositive women, of whom 22 were HIV-1 seropositive, were followed up weekly for 3 weeks for assessment of HIV-1 and HSV-2 genital shedding in Bobo Dioulasso, Burkina Faso. HSV-2 DNA and HIV-1 RNA were quantitated in cervicovaginal lavages enriched with a cervical swabbing (eCVL) using real-time polymerase chain reaction. HIV-1 RNA shedding was detected in eCVL from 19 of 22 (86.4%) of the HIV-1-seropositive women and HSV-2 DNA was detected in eCVL from 12 of 42 (28.6%) of the women. Compared with the HIV-1-seronegative women, the HIV-1-seropositive women showed more persistent HSV-2 genital shedding and higher HSV-2 DNA loads in eCVL. This study showed that eCVL is a reliable sampling method for detecting both viruses. Three measurement points (at weekly intervals in this study) seem to adequately allow detection of most HIV-1 or HSV-2 genital shedders.
Nagot
Nicolas
N
Centre Muraz, Bobo Dioulasso, Burkina Faso.
Foulongne
Vincent
V
Becquart
Pierre
P
Mayaud
Philippe
P
Konate
Issouf
I
Ouedraogo
Abdoulaye
A
Defer
Marie-Christine
MC
Weiss
Helen
H
Van de Perre
Philippe
P
Segondy
Michel
M
D000328:Adult; D015331:Cohort Studies; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D005836:Genitalia, Female / Q000821:virology*; D015658:HIV Infections / Q000175:diagnosis; D015497:HIV-1 / Q000302:isolation & purification*; D006558:Herpes Genitalis / Q000175:diagnosis; D018258:Herpesvirus 2, Human / Q000302:isolation & purification*; D006801:Humans; D008137:Longitudinal Studies; D008875:Middle Aged; D011446:Prospective Studies; D012367:RNA, Viral / Q000302:isolation & purification; D007507:Therapeutic Irrigation; D014626:Vaginal Smears / Q000379:methods*; D019562:Viral Load; D017201:Virus Shedding
D004279:DNA, Viral; D012367:RNA, Viral