title: Structural and functional analysis of 5S rRNA in Saccharomyces cerevisiae.
abstract: 5S rRNA extends from the central protuberance of the large ribosomal subunit, through the A-site finger, and down to the GTPase-associated center. Here, we present a structure-function analysis of seven 5S rRNA alleles which are sufficient for viability in the yeast Saccharomyces cerevisiae when expressed in the absence of wild-type 5S rRNAs, and extend this analysis using a large bank of mutant alleles that show semi-dominant phenotypes in the presence of wild-type 5S rRNA. This analysis supports the hypothesis that 5S rRNA serves to link together several different functional centers of the ribosome. Data are also presented which suggest that in eukaryotic genomes selection has favored the maintenance of multiple alleles of 5S rRNA, and that these may provide cells with a mechanism to post-transcriptionally regulate gene expression.
authors.affiliation: Department of Chemistry, Moscow State University, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Kiparisov
authors.forename: Alexey
authors.initials: A
authors.lastname: Petrov
authors.forename: Arturas
authors.initials: A
authors.lastname: Meskauskas
authors.forename: Petr V
authors.initials: PV
authors.lastname: Sergiev
authors.forename: Olga A
authors.initials: OA
authors.lastname: Dontsova
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Dinman
mesh_terms: D000483:Alleles; D020029:Base Pairing; D001483:Base Sequence; D018965:Frameshifting, Ribosomal / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000235:genetics*; D012270:Ribosomes / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D012341:RNA, Ribosomal, 5S