title: [Recombinant human pigment epithelium-derived factor inhibits the proliferation of the endothelial cells from blood vessels].
abstract: OBJECTIVE
  To express and purify the recombinant human pigment epithelium-derived factor (PEDF) which inhibits the proliferation of the endothelium cells from blood vessel in E.coli.
  
  
  METHODS
  PEDF gene was inserted into the prokaryotic expression vector pGEX-4T-2. The recombinant protein PEDF was expressed in E.coli BL-21, and purified by the GST Sepharose 4B affinity column. The recombinant human PEDF protein was identified by Western blot and mass spectrum. The biological activity of the recombinant human PEDF protein was measured by using MTT.
  
  
  RESULTS
  The 46 kDa recombinant human PEDF protein was obtained. It significantly inhibited the proliferation of the human umbilical vein cell line HUVEC.
  
  
  CONCLUSIONS
  The recombinant human PEDF with anti-angiogenesis activity protein may be successfully purify through prokaryotic expression.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
authors.forename: Fei-chan
authors.initials: FC
authors.lastname: Qiu
authors.forename: Zhong-wei
authors.initials: ZW
authors.lastname: Wen
authors.forename: Sheng-tao
authors.initials: ST
authors.lastname: Zhu
authors.forename: Jian-gang
authors.initials: JG
authors.lastname: Yuan
authors.forename: Bo-qin
authors.initials: BQ
authors.lastname: Qiang
authors.forename: Xiao-zhong
authors.initials: XZ
authors.lastname: Peng
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004730:Endothelium, Vascular / Q000166:cytology*; D005136:Eye Proteins / Q000494:pharmacology*; D006801:Humans; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology*; D011387:Prokaryotic Cells / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D015843:Serpins / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D020533:Angiogenesis Inhibitors; D005136:Eye Proteins; D009414:Nerve Growth Factors; D011994:Recombinant Proteins; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor