title: Protective effect of polyethylene glycol-superoxide dismutase on leukocyte dynamics in rat retinal microcirculation under lipid hydroperoxide-induced oxidative stress.
abstract: The levels of lipid hydroperoxide (LHPs) in vitreous are elevated in a variety of retinal disorders. Recently, we have shown that increased levels of LHPs in the vitreous enhanced leukocyte-endothelium interaction in the retina, which should contribute to the initial disturbance of the retinal microcirculation. Based upon the previous work, the purpose of the present study was to investigate the effect of polyethylene glycol-superoxide dismutase (PEG-SOD), one of the important enzyme antioxidants, on leukocyte-endothelial interaction in the retinal microcirculation under LHP-induced oxidative stress. Male Brown-Norway rats weighing approximately 250 g were used. LHP(18:2) was made from linoleic acid (LA) with lipoxygenase and 10 microg of LHP dissolved in 5 microl of sodium borate buffer (SBB, 0.02 m) was slowly injected into the vitreous using a 33-gauge needle. PEG-SOD (5000 units/kg) was given intravenously 5 min before LHP injection. At 2, 4, 6, 12, 24 and 48 hr after the vitreous injections, we evaluated the number of rolling leukocytes along the major retinal veins and the number of leukocytes that accumulated in the retinal microvasculature with acridine orange digital fluorography. In LHP-treated rats, leukocyte rolling along the major retinal veins was maximal at 6 hr after LHP injection. The number of rolling leukocytes in the PEG-SOD-treated rats was decreased to 5.5% of those in the LHP-treated rats at 6 hr after LHP injection (P<0.01). No rolling leukocytes were observed in either control or vehicle-treated eyes. The number of accumulated leukocytes in LHP-treated eyes started to increase at 12 hr, and peaked at 24 hr which was significantly higher than in both control and vehicle-treated eyes (P<0.01). The number of accumulated leukocytes in the PEG-SOD-treated rats was reduced by 88.0% at 24 hr (P<0.01). Intravenous injection of PEG-SOD significantly inhibited the leukocyte rolling and its accumulation under LHP-induced oxidative stress. These results suggest that PEG-SOD might attenuate various retinal microcirculatory disorders associated with LHP.
authors.affiliation: Department of Ophthalmology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. akihisa_matsubara@meei.harvard.edu
authors.forename: Akihisa
authors.initials: A
authors.lastname: Matsubara
authors.forename: Kazushi
authors.initials: K
authors.lastname: Tamai
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Yuji
authors.initials: Y
authors.lastname: Niwa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Morita
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Tomida
authors.forename: Donald
authors.initials: D
authors.lastname: Armstrong
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Ogura
mesh_terms: D000818:Animals; D007091:Image Processing, Computer-Assisted; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects*; D008054:Lipid Peroxides / Q000494:pharmacology*; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D011914:Rats, Inbred BN; D012171:Retinal Vessels / Q000187:drug effects* / Q000502:physiology; D013482:Superoxide Dismutase / Q000494:pharmacology*; D014664:Vasodilation / Q000187:drug effects
chemical_list: D008054:Lipid Peroxides; D011092:Polyethylene Glycols; D013482:Superoxide Dismutase; C063471:polyethylene glycol-superoxide dismutase