title: Molecular analysis of intrafamiliar transmission of Moraxella catarrhalis.
abstract: The possible intrafamiliar transmission of Moraxella catarrhalis was evaluated in 3 pairs between children and their parents, and 8 pairs between siblings from 11 families. Of the 22 isolates, all were found producing beta-lactamase. Molecular typing by pulsed-field gel electrophoresis (PFGE) with Not I and Spe I showed that the PFGE patterns in 2 of 3 pairs between children and their parents, and 4 of 8 pairs between siblings were indistinguishable and those of the remaining pairs were different. These data indicate a possible high rate of intrafamiliar transmission of M. catarrhalis.
authors.affiliation: Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. h-wata@net.nagasaki-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Hoshino
authors.forename: Rinya
authors.initials: R
authors.lastname: Sugita
authors.forename: Norichika
authors.initials: N
authors.lastname: Asoh
authors.forename: Heinner
authors.initials: H
authors.lastname: Guio
authors.forename: Liang
authors.initials: L
authors.lastname: Qin
authors.forename: Chiharu
authors.initials: C
authors.lastname: Kaji
authors.forename: Kiwao
authors.initials: K
authors.lastname: Watanabe
authors.forename: Kazunori
authors.initials: K
authors.lastname: Oishi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nagatake
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D005192:Family Health; D006801:Humans; D007223:Infant; D007564:Japan; D017720:Molecular Epidemiology; D001936:Moraxella catarrhalis / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D045828:Moraxellaceae Infections / Q000382:microbiology* / Q000635:transmission*; D012150:Polymorphism, Restriction Fragment Length; D001618:beta-Lactamases / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; C056436:endodeoxyribonuclease SpeI; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases; D001618:beta-Lactamases