title: Metagenomic marine nitrogen fixation--feast or famine?
abstract: Natural populations of bacteria in different environments can be astonishingly diverse, as was revealed graphically by large-scale sequencing of samples of their so-called metagenomes. Among the sequence datasets from four different samples of marine bacterial metagenomes, we noted that nitrogen fixation (nif) genes were conspicuous by their absence from three of them. However, in one sample, more than one-third of the bacteria appeared to have a complement of these genes. Here, some reasons behind this site-to-site variability and their implications for how molecular methods, involving large-scale sequencing and/or functional metagenomics, can best be used to describe bacterial diversity in natural environments are discussed.
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK. a.johnston@uea.ac.uk
authors.forename: Andrew W B
authors.initials: AW
authors.lastname: Johnston
authors.forename: Youguo
authors.initials: Y
authors.lastname: Li
authors.forename: Lesley
authors.initials: L
authors.lastname: Ogilvie
mesh_terms: D001419:Bacteria / Q000235:genetics*; D044822:Biodiversity; D019295:Computational Biology; D030541:Databases, Genetic; D016680:Genome, Bacterial; D023281:Genomics; D009586:Nitrogen Fixation / Q000235:genetics*; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology