title: Geographical distribution of genetic polymorphism of the pathogen Histoplasma capsulatum isolated from infected bats, captured in a central zone of Mexico.
abstract: Fourteen Histoplasma capsulatum isolates recovered from infected bats captured in Mexican caves and two human H. capsulatum reference strains were analyzed using random amplification of polymorphic DNA PCR-based and partial DNA sequences of four genes. Cluster analysis of random amplification of polymorphic DNA-patterns revealed differences for two H. capsulatum isolates of one migratory bat Tadarida brasiliensis. Three groups were identified by distance and maximum-parsimony analyses of arf, H-anti, ole, and tub1 H. capsulatum genes. Group I included most isolates from infected bats and one clinical strain from central Mexico; group II included the two isolates from T. brasiliensis; the human G-217B reference strain from USA formed an independent group III. Isolates from group II showed diversity in relation to groups I and III, suggesting a different H. capsulatum population.
authors.affiliation: Laboratorio de Inmunología de Hongos, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria. emello@servidor.unam.mx
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Taylor
authors.forename: Catalina B
authors.initials: CB
authors.lastname: Chávez-Tapia
authors.forename: Alberto
authors.initials: A
authors.lastname: Rojas-Martínez
authors.forename: Maria
authors.initials: M
authors.lastname: del Rocio Reyes-Montes
authors.forename: Mirian Bobadilla
authors.initials: MB
authors.lastname: del Valle
authors.forename: Gerardo
authors.initials: G
authors.lastname: Zúñiga
mesh_terms: D000818:Animals; D002685:Chiroptera / Q000382:microbiology*; D005656:Fungal Proteins / Q000235:genetics; D006658:Histoplasma / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006660:Histoplasmosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012015:Reference Standards; D017422:Sequence Analysis, DNA
chemical_list: D005656:Fungal Proteins