A convenient method for preparation of high-purity, Amadori-glycated phosphatidylethanolamine and its prooxidant effect.
Phospholipid-linked glycation has been implicated to play an important role in lipid peroxidation associated with several conditions, such as normal aging and diabetes. In this study, we established a convenient method for the preparation of a high-purity, Amadori-glycated phosphatidylethanolamine (Amadori-PE) standard in a methanol system. We evaluated the prooxidant effect of the Amadori-PE standard. When Amadori-PE was incubated in a micellar and liposome system in the presence of metal ion, a remarkable increase of lipid peroxidation was observed in both systems. In addition, the Amadori-PE-induced lipid peroxidation was effectively inhibited by superoxide dismutase, mannitol, catalase, and EDTA. These results indicate that Amadori-PE is a considerable risk factor that may cause membrane lipid peroxidation in the pathogenesis of diabetes and aging.
Miyazawa
Teruo
T
Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan. miyazawa@biochem.tohoku.ac.jp
Oak
Jeong-Ho
JH
Nakagawa
Kiyotaka
K
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006031:Glycosylation; D015227:Lipid Peroxidation; D008081:Liposomes; D008823:Micelles; D016877:Oxidants; D010714:Phosphatidylethanolamines
D008081:Liposomes; D008823:Micelles; D016877:Oxidants; D010714:Phosphatidylethanolamines