[Laboratory diagnosis of avian influenza by reverse transcription (RT)-PCR].
RT-PCR assays which amplify conserved regions of the influenza A virus gene are useful tools for the rapid and specific detection of infections of poultry with avian influenza virus (AIV) and for the investigation of large numbers of samples, e.g. within the framework of surveillance programs. Here, we present findings on the efficiency and on the limits of an RT-PCR assay which amplifies a part of the matrix protein gene. Sensitivity and specificity of the method were increased by the additional use of nested PCR. Parameters which may have an essential influence on the detection limit are outlined and discussed. A major focus of the study is the detection of AIV RNA from organ samples and swabs.
Starick
Elke
E
Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit, Insel Riems. elke.starick@rie.bfav.de
Werner
Ortrud
O
Kaden
Volker
V
D000818:Animals; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis*; D011200:Poultry; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D012367:RNA, Viral