Evidence for localisation of accumulated chlorophyll cation on the D1-accessory chlorophyll in the reaction centre of photosystem II.
Absorption and circular dichroism spectra of Photosystem II (PS II) reaction centres (RC) were studied and compared with spectra calculated on the basis of point-dipole approximation. Chlorophyll cation was accumulated during a light treatment of PS II RC in the presence of artificial electron acceptor silicomolybdate. Light-induced difference spectra and their calculated counterparts revealed the location of accumulated cation at the accessory chlorophyll of the D1 protein subunit.
Vacha
Frantisek
F
Institute of Physical Biology, University of South Bohemia, Zamek 136, 373 33 Nove Hrady, Czech Republic. vacha@jcu.cz
Psencik
Jakub
J
Kuty
Michal
M
Durchan
Milan
M
Siffel
Pavel
P
D002412:Cations / Q000737:chemistry / Q000378:metabolism; D002734:Chlorophyll / Q000737:chemistry* / Q000378:metabolism*; D002942:Circular Dichroism; D004735:Energy Transfer; D008027:Light; D008954:Models, Biological; D018532:Pisum sativum / Q000737:chemistry / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D013054:Spectrophotometry, Atomic; D013696:Temperature
D002412:Cations; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D002734:Chlorophyll