title: Molecular cloning and expression analysis of two hepcidin genes from olive flounder Paralichthys olivaceus.
abstract: Hepcidin is a cysteine-rich cationic antimicrobial peptide central to iron metabolism. We report a comparative analysis of the sequences, gene organization and expression of two hepcidin genes from olive flounder Paralichthys olivaceus. Both consist of two introns and three exons that encode a prepropeptide (81 amino acids for hepcidin I and 89 amino acids for hepcidin II). A TATA box and several consensus-binding motifs for transcription factors were found upstream of the transcriptional starting site. Hepcidin II was predominantly expressed in the liver and highly inducible under the effect of lipopolysaccharide (LPS), while a large amount of hepcidin I transcripts was detected in various tissues but did not appear to have a significant effect during LPS-stimulation.
authors.affiliation: Biotechnology Research Center, National Fisheries Research and Development Institute, 408-1 Gijang, Busan 619-0902, Korea. yokim@momaf.go.kr
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Kim
authors.forename: Suhee
authors.initials: S
authors.lastname: Hong
authors.forename: Bo-Hye
authors.initials: BH
authors.lastname: Nam
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Kyung-Kil
authors.initials: KK
authors.lastname: Kim
authors.forename: Sang-Jun
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics*; D005432:Flounder / Q000235:genetics*; D020869:Gene Expression Profiling; D064451:Hepcidins; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D029941:Fish Proteins; D064451:Hepcidins; D020033:Protein Isoforms; D012313:RNA; D004247:DNA