title: Bacterial ghosts as an oral vaccine: a single dose of Escherichia coli O157:H7 bacterial ghosts protects mice against lethal challenge.
abstract: Enterohemorrhagic Escherichia coli (EHEC) is a bacterial pathogen that is associated with several life-threatening diseases for humans. The combination of protein E-mediated cell lysis to produce EHEC ghosts and staphylococcal nuclease A to degrade DNA was used for the development of an oral EHEC vaccine. The lack of genetic material in the oral EHEC bacterial-ghost vaccine abolished any hazard of horizontal gene transfer of resistance genes or pathogenic islands to resident gut flora. Intragastric immunization of mice with EHEC ghosts without the addition of any adjuvant induced cellular and humoral immunity. Immunized mice challenged at day 55 showed 86% protection against lethal challenge with a heterologous EHEC strain after single-dose oral immunization and 93.3% protection after one booster at day 28, whereas the controls showed 26.7% and 30% survival, respectively. These results indicate that it is possible to develop an efficacious single-dose oral EHEC bacterial-ghost vaccine.
authors.affiliation: Faculty of Life Science, University of Vienna, Department of Medical/Pharmaceutical Chemistry, Althanstrasse 14, UZAII, 2B522, 1090 Vienna, Austria. ulrike.beate.mayr@univie.ac.at
authors.forename: Ulrike Beate
authors.initials: UB
authors.lastname: Mayr
authors.forename: Christoph
authors.initials: C
authors.lastname: Haller
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Haidinger
authors.forename: Alena
authors.initials: A
authors.lastname: Atrasheuskaya
authors.forename: Eugenij
authors.initials: E
authors.lastname: Bukin
authors.forename: Werner
authors.initials: W
authors.lastname: Lubitz
authors.forename: Georgy
authors.initials: G
authors.lastname: Ignatyev
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000276:immunology*; D049109:Cell Proliferation; D003106:Colon / Q000276:immunology; D004927:Escherichia coli Infections / Q000276:immunology / Q000401:mortality / Q000517:prevention & control*; D019453:Escherichia coli O157 / Q000276:immunology* / Q000648:ultrastructure; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022223:Vaccines, Subunit; D007371:Interferon-gamma