Characterization of a humanized monoclonal antibody recognizing clumping factor A expressed by Staphylococcus aureus.
We report the humanization and characterization of monoclonal antibody (MAb) T1-2 or tefibazumab, a monoclonal antibody that recognizes clumping factor A expressed on the surface of Staphylococcus aureus. We demonstrate that the binding kinetics of MAb T1-2 is indistinguishable compared to that of its murine parent. Furthermore, MAb T1-2 is shown to enhance the opsonophagocytic uptake of ClfA-coated latex beads, protect against an intravenous challenge in a prophylactic model of rabbit infective endocarditis, and enhance the efficacy of vancomycin therapy in a therapeutic model of established infective endocarditis.
Domanski
Paul J
PJ
Inhibitex, Inc., 9005 Westside Parkway, Suite 400, Alpharetta, GA 30004, USA.
Patel
Pratiksha R
PR
Bayer
Arnold S
AS
Zhang
Li
L
Hall
Andrea E
AE
Syribeys
Peter J
PJ
Gorovits
Elena L
EL
Bryant
Dawn
D
Vernachio
John H
JH
Hutchins
Jeff T
JT
Patti
Joseph M
JM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood / Q000276:immunology*; D002460:Cell Line; D003030:Coagulase / Q000276:immunology*; D006801:Humans; D011817:Rabbits; D013211:Staphylococcus aureus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003030:Coagulase