OD1, the first toxin isolated from the venom of the scorpion Odonthobuthus doriae active on voltage-gated Na+ channels.
In this study, we isolated and pharmacologically characterized the first alpha-like toxin from the venom of the scarcely studied Iranian scorpion Odonthobuthus doriae. The toxin was termed OD1 and its primary sequence was determined: GVRDAYIADDKNCVYTCASNGYCNTECTKNGAESGYCQWIGRYGNACWCIKLPDEVPIRIPGKCR. Using the two-electrode voltage clamp technique, the pharmacological effects of OD1 were studied on three cloned voltage-gated Na+ channels expressed in Xenopus laevis oocytes (Na(v)1.2/beta1, Na(v)1.5/beta1, para/tipE). The inactivation process of the insect channel, para/tipE, was severely hampered by 200 nM of OD1 (EC50 = 80+/-14 nM) while Na(v)1.2/beta1 still was not affected at concentrations up to 5 microM. Na(v)1.5/beta1 was influenced at micromolar concentrations.
Jalali
Amir
A
Department of Toxicology and Pharmacology, Shaheed Beheshti University of Medical Science, Tehran, Iran.
Bosmans
Frank
F
Amininasab
Mehriar
M
Clynen
Elke
E
Cuypers
Eva
E
Zaremirakabadi
Abbas
A
Sarbolouki
Mohammad-Nabi
MN
Schoofs
Liliane
L
Vatanpour
Hossein
H
Tytgat
Jan
J
D000595:Amino Acid Sequence; D000818:Animals; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D012604:Scorpion Venoms / Q000737:chemistry*; D012605:Scorpions; D017386:Sequence Homology, Amino Acid; D015222:Sodium Channels / Q000187:drug effects*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009498:Neurotoxins; D012604:Scorpion Venoms; D015222:Sodium Channels