title: RNase P cleaves transient structures in some riboswitches.
abstract: RNase P from Escherichia coli cleaves the coenzyme B12 riboswitch from E. coli and a similar one from Bacillus subtilis. The cleavage sites do not occur in any recognizable structure, as judged from theoretical schemes that have been drawn for these 5' UTRs. However, it is possible to draw a scheme that is a good representation of the E. coli cleavage site for RNase P and for the cleavage site in B. subtilis. These data indicate that transient structures are important in RNase P cleavage and in riboswitch function. Coenzyme B12 has a small inhibitory effect on E. coli RNase P cleavage of the E. coli riboswitch. Both E. coli RNase P and a partially purified RNase P from Aspergillus nidulans mycelia succeeded in cleaving a putative arginine riboswitch from A. nidulans. The cleavage site may be a representative of another model substrate for eukaryotic RNase P. This 5' UTR controls splicing of the arginase mRNA in A. nidulans. Four other riboswitches in E. coli were not cleaved by RNase P under the conditions tested.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA. sidney.altman@yale.edu
authors.forename: Sidney
authors.initials: S
authors.lastname: Altman
authors.forename: Donna
authors.initials: D
authors.lastname: Wesolowski
authors.forename: Cecilia
authors.initials: C
authors.lastname: Guerrier-Takada
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D001233:Aspergillus nidulans; D001412:Bacillus subtilis; D020029:Base Pairing; D001483:Base Sequence; D003001:Cloning, Molecular; D003038:Cobamides / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers; D004926:Escherichia coli; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D043262:Ribonuclease P / Q000378:metabolism*; D001616:beta-Galactosidase
chemical_list: D003038:Cobamides; D017931:DNA Primers; D012333:RNA, Messenger; D043262:Ribonuclease P; D001616:beta-Galactosidase; C000913:cobamamide