An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants.
A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.
Schmälzlin
Elmar
E
Institute of Chemistry, University of Potsdam, Germany. schmaelzlin@chem.uni-potsdam.de
van Dongen
Joost T
JT
Klimant
Ingo
I
Marmodée
Bettina
B
Steup
Martin
M
Fisahn
Joachim
J
Geigenberger
Peter
P
Löhmannsröben
Hans-Gerd
HG
D044784:Chara / Q000378:metabolism*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007424:Intracellular Fluid / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010100:Oxygen / Q000032:analysis*; D010101:Oxygen Consumption / Q000502:physiology*; D010944:Plants / Q000378:metabolism*
D010100:Oxygen