title: Altered protein expression in endometrial carcinogenesis.
abstract: We have discovered several protein biomarkers that are altered during carcinogenesis of the human uterine endometrium. Proteins prepared from 19 endometrial carcinomas (Group A), and 20 normal endometria obtained from benign gynecological conditions (Group B), were investigated by Surface Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF-MS). Two proteins, EC1 and EC2, were consistently expressed differentially. EC1 had an increased level of expression in carcinomas (P<0.001), while EC2 was expressed at a lower level (P=0.004). The isoelectric points of EC1 and EC2 were approximately pH 5.0 and 7.0, respectively. These proteins are thus potential biomarkers for detection of endometrial carcinoma.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Yoshizaki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Enomoto
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Nakashima
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ueda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kanao
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yoshino
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fukumoto
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoneda
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Buzard
authors.forename: Yuji
authors.initials: Y
authors.lastname: Murata
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008875:Middle Aged; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor