The csnD/csnE signalosome genes are involved in the Aspergillus nidulans DNA damage response.
The signalosome (CSN) is a conserved multiprotein complex involved in regulation of eukaryotic development and is also required to activate ribonucleotide reductase for DNA synthesis. In Aspergillus nidulans, csnD/csnE are key regulators of sexual development. Here, we investigated whether the csnD/csnE genes are involved in the DNA damage response in this fungus. The growth of the csnD/csnE deletion mutants was reduced by subinhibitory concentrations of hydroxyurea, camptothecin, 4-nitroquinoline oxide, and methyl methanesulfonate. A. nidulans increases csnD/csnE mRNA levels when it is challenged by different DNA-damaging agents. There is no significant transcriptional induction of the csnE promoter fused with lacZ gene in the presence of DNA-damaging agents, suggesting that increased mRNA accumulation is due to increased mRNA stability. Septation was not inhibited in the csnD/csnE deletion mutants while DeltauvsB DeltacsnE presented an increase in septation upon DNA damage caused by methyl methanesulfonate, suggesting that uvsB(ATR) and csnE genetically interact during checkpoint-dependent inhibition of septum formation. The double DeltacsnD/DeltacsnE DeltanpkA mutants were more sensitive to DNA-damaging agents than were the respective single mutants. Our results suggest that csnD/csnE genes are involved in the DNA damage response and that NpkA and UvsB(ATR) genetically interact with the signalosome.
Lima
Joel Fernandes
JF
Faculdade de Ciências Farmacêuticas de Ribeirão Preto and Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, 1404-903 Brazil.
Malavazi
Iran
I
von Zeska Kress Fagundes
Marcia Regina
MR
Savoldi
Marcela
M
Goldman
Maria Helena S
MH
Schwier
Elke
E
Braus
Gerhard H
GH
Goldman
Gustavo Henrique
GH
D001233:Aspergillus nidulans / Q000235:genetics* / Q000502:physiology; D000075686:COP9 Signalosome Complex; D004249:DNA Damage / Q000502:physiology*; D004260:DNA Repair / Q000235:genetics* / Q000502:physiology; D005800:Genes, Fungal; D046912:Multiprotein Complexes / Q000235:genetics*; D010447:Peptide Hydrolases / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D012098:Reproduction / Q000235:genetics; D012264:Ribonucleotide Reductases / Q000096:biosynthesis / Q000235:genetics
D046912:Multiprotein Complexes; D012333:RNA, Messenger; D012264:Ribonucleotide Reductases; D010447:Peptide Hydrolases; D000075686:COP9 Signalosome Complex