title: Bone formation using human adipose tissue-derived stromal cells and a biodegradable scaffold.
abstract: Human adipose tissue, obtained by liposuction, was processed to obtain a fibroblast-like population of cells or adipose tissue-derived stromal cells (ATSCs). The ATSCs, as well as bone marrow-derived mesenchymal stem cells (BMSCs), have the capacity for renewal and the potential to differentiate into multiple lineages of mesenchymal tissues. These cells are capable of forming bone when implanted ectopically in an appropriate scaffold. The aim of this study was to evaluate a beta-tricalcium phosphate (beta-TCP) as a scaffold and to compare the potential of osteogenic differentiation of ATSCs with BMSCs. Both cell types were loaded into beta-TCP disk and cultured in an osteogenic induction medium. Optimal osteogenic differentiation in ATSCs in vitro, as determined by secretion of osteocalcin, scanning electron microscope, and histology, were obtained in the culturing with the beta-TCP disk. Furthermore, bone formation in vivo was examined by using the ATSC- or BMSC-loaded scaffolds in nude mice. The present results show that ATSCs have a similar ability to differentiate into osteoblasts and to synthesize bone in beta-TCP disk as have BMSCs.
authors.affiliation: Division of Biomedical Engineering, Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: Hidemi
authors.initials: H
authors.lastname: Hattori
authors.forename: Kazunori
authors.initials: K
authors.lastname: Masuoka
authors.forename: Masato
authors.initials: M
authors.lastname: Sato
authors.forename: Miya
authors.initials: M
authors.lastname: Ishihara
authors.forename: Takashi
authors.initials: T
authors.lastname: Asazuma
authors.forename: Bonpei
authors.initials: B
authors.lastname: Takase
authors.forename: Makoto
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Nemoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D001846:Bone Development; D002130:Calcium Phosphates; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D017154:Stromal Cells / Q000166:cytology*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate