Methods for the culture of C. elegans and S. cerevisiae in microgravity.
To support the study of the effects of microgravity on biological systems, our group is developing and testing methods that allow the cultivation of C. elegans and S. cerevisiae in microgravity. Our aim is to develop the experimental means by which investigators may conduct peer reviewed biological experiments with C. elegans or S. cerevisiae in microgravity. Our protocols are aimed at enabling investigators to grow these organisms for extended periods during which samples may be sub-cultured, collected, preserved, frozen, and/or returned to earth for analysis. Data presented include characterization of the growth phenotype of these organisms in liquid medium in OptiCells(TM) (Biocrystal, LTD).
Fahlen
Thomas
T
Lockheed Martin Space Operations, NASA Ames Research Center, Moffett Field, CA 94035, USA.
Sunga
June
J
Rask
Jon
J
Herrera
Anna
A
Lam
Kitty
K
Sing
Luke
L
Sato
Kevin
K
Ramos
Ross A
RA
Kirven-Brooks
Melissa
M
Reiss-Bubenheim
Debra
D
D000818:Animals; D017173:Caenorhabditis elegans / Q000254:growth & development*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D005069:Evaluation Studies as Topic; D008021:Life Support Systems; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D014893:Weightlessness
D003470:Culture Media