title: Brown adipose tissue mitochondrial subpopulations show different morphological and thermogenic characteristics.
abstract: Rat brown adipose tissue mitochondrial subpopulations-isolated by differential centrifugation at 1000, 3000 and 8000g, giving the heavy, medium and light mitochondria-were characterized. Thus, contamination by non-mitochondrial subcellular components, morphological features, respiratory chain and antioxidant enzyme activities, both uncoupling protein 1 and mitochondrial protein content, mitochondrial DNA levels and mitochondrial integrity were measured. Results indicate that mitochondrial fractions showed important differences in the morphological, thermogenic and antioxidant properties. All the parameters studied were always higher in heavy mitochondria, which is indicative of a greater mitochondrial differentiation state.
authors.affiliation: Departament de Biologia Fonamental i Ciències de la Salut, Institut Universitari d'lnvestigacióen Ciències de la Salut (IUNICS), Universitat de les Illes Balears, E-07122 Palma de Mallorca, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Justo
authors.forename: Jordi
authors.initials: J
authors.lastname: Oliver
authors.forename: Magdalena
authors.initials: M
authors.lastname: Gianotti
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism* / Q000648:ultrastructure*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001483:Base Sequence; D002458:Cell Fractionation; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D008854:Microscopy, Electron; D008928:Mitochondria / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure*; D024101:Mitochondrial Proteins / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D022722:Thermogenesis
chemical_list: D000975:Antioxidants; D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins