title: A monoclonal antibody-based ELISA allows discrimination between responses induced by bovine herpesvirus subtypes 1 (BoHV-1.1) and 2 (BoHV-1.2).
abstract: Bovine herpesvirus type 1 (BoHV-1) has distinct subtypes according to genomic characterization. Immune responses induced by BoHV-1 subtype 1 (BoHV-1.1) are not distinguishable from those induced by BoHV-1 subtype 2 (BoHV-1.2) through conventional serological methods. In the present report, an enzyme linked immunosorbent assay is described that allows discrimination between immune responses in cattle immunized with either subtype, based on a monoclonal antibody that recognizes specifically the amino-terminal region of glycoprotein C (gC) on BoHV-1.1 strains, thus not reacting with BoHV-1.2a. The test displayed a sensitivity of 92%, specificity of 90% and a good correlation with serum neutralization tests on samples from BoHV-1.1-immunized calves (kappa = 0.799). The test may be useful to provide new insights into the roles played by each of these two subtypes in the epidemiology of BoHV-1 infections.
authors.affiliation: Centro de Pesquisas Veterinárias Desidério Finamor (CPVDF/FEPAGRO), Eldorado do Sul, Brazil. spilki@unicamp.br
authors.forename: Fernando Rosado
authors.initials: FR
authors.lastname: Spilki
authors.forename: Paulo Augusto
authors.initials: PA
authors.lastname: Esteves
authors.forename: Alessandra D'Avila
authors.initials: AD
authors.lastname: da Silva
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Franco
authors.forename: Frans A M
authors.initials: FA
authors.lastname: Rijsewijk
authors.forename: Paulo Michel
authors.initials: PM
authors.lastname: Roehe
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000918:Antibody Specificity; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006561:Herpes Simplex / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology*; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D001911:Herpesvirus 2, Bovine / Q000276:immunology*; D007114:Immunization; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology*; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins