title: An insertion/deletion polymorphism in the gene encoding angiotensin converting enzyme is not associated with generalised vitiligo in an English population.
abstract: Vitiligo is an acquired hypomelanotic skin disorder characterised by circumscribed depigmented macules resulting from the loss of functional melanocytes from the cutaneous epidermis and autoimmunity has been suggested to play a role in the pathogenesis of the disease. Recently, an insertion/deletion (I/D) polymorphism of a 287-base pair repetitive sequence in intron 16 of the angiotensin converting enzyme (ACE) gene has been associated with autoimmune disease and with the development of vitiligo. In this study, the distribution of ACE gene I/D genotypes was investigated in a population of 106 English patients with generalised (non-segmental) vitiligo and 174 ethnically matched healthy controls using a restriction fragment length polymorphism-polymerase chain reaction genotyping method. No significant difference in the frequencies of II, ID and DD genotypes was detected between vitiligo patients and control subjects (P=0.35). The same result was evident for the genotype distribution in vitiligo patients with an autoimmune disease and for those without when compared with controls (P=0.33 and P=0.53, respectively). In addition, the results indicated that the D allele was not significantly over-represented in the group of patients with vitiligo compared with controls (P=0.42) and that this was also the case for patients with and without associated autoimmunity (P=0.40 and P=0.62, respectively).
authors.affiliation: Division of Clinical Sciences (North), University of Sheffield, Northern General Hospital, Herries Road, Sheffield S5 7AU, UK.
authors.forename: Samia
authors.initials: S
authors.lastname: Akhtar
authors.forename: Nikos G
authors.initials: NG
authors.lastname: Gavalas
authors.forename: David J
authors.initials: DJ
authors.lastname: Gawkrodger
authors.forename: Philip F
authors.initials: PF
authors.lastname: Watson
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Weetman
authors.forename: E Helen
authors.initials: EH
authors.lastname: Kemp
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004739:England; D005260:Female; D017353:Gene Deletion; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014820:Vitiligo / Q000235:genetics*; D044465:White People
chemical_list: D007703:Peptidyl-Dipeptidase A