title: Purification and characterization of dioscin-alpha-L-rhamnosidase from pig liver.
abstract: Dioscin-alpha-L-rhamnosidase was isolated, purified and partially characterized from pig liver. The maximum activity was reached at pH 7, 42 degrees C, 24 h, and 2% of substrate concentration. Fe3+ and Cu2+ inhibited the enzyme; the ion Ca2+ activated it. Mg2+ was an inhibitor at 100 mM, but it was an activator at 200 mM. Zn2+ could be a weak activator of the enzyme. The molecular weight of dioscin-alpha-L-rhamnosidase was about 47 kDa as determined by the method of SDS-polyacrylamide gel electrophoresis.
authors.affiliation: College of Food Science, Shenyang Agricultural University, Dongling-lu No. 120, Dongling-qu, Shenyang 110161, PR China.
authors.forename: Srguleng
authors.initials: S
authors.lastname: Qian
authors.forename: Hongshan
authors.initials: H
authors.lastname: Yu
authors.forename: Chunzhi
authors.initials: C
authors.lastname: Zhang
authors.forename: Mingchun
authors.initials: M
authors.lastname: Lu
authors.forename: Hongying
authors.initials: H
authors.lastname: Wang
authors.forename: Fengxie
authors.initials: F
authors.lastname: Jin
mesh_terms: D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002855:Chromatography, Thin Layer; D004144:Diosgenin / Q000031:analogs & derivatives / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D013552:Swine
chemical_list: C019357:dioscin; D006026:Glycoside Hydrolases; C504488:dioscin-alpha-L-rhamnosidase, pig; D004144:Diosgenin