Specific formation of beads-on-a-chain structures on giant DNA using a designed polyamine derivative.
Fluorescence microscopy was used to study the folding transition of giant DNAs, T4 DNA (ca. 166 kbp), and lambda DNA (ca. 48 kbp), which proceeds through intermediates with intramolecular segregation induced by pteridine-polyamine conjugates, i.e., 2-amino-6,7-dimethyl-4-(4,9,13-triazatridecylamino)pteridine and -4-(3-(aminopropyl)amino)pteridine. According to the results of DNA denaturation, UV and fluorescent spectroscopy, and transmission electron microscopic observations, it became clear that DNA folding induced by the polyamine derivative is not a continuous shrinking process but a combination of discontinuous processes.
Chen
Ning
N
Graduate School of Environmental Studies, Nagoya University, Chikusa, Nagoya 464-8601 Japan.
Zinchenko
Anatoly A
AA
Murata
Shizuaki
S
Yoshikawa
Kenichi
K
D017122:Bacteriophage T4 / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D009691:Nucleic Acid Denaturation; D011073:Polyamines / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D004279:DNA, Viral; D011073:Polyamines