Synergistic recognition of an epigenetic DNA element by Pleiohomeotic and a Polycomb core complex.
Polycomb response elements (PREs) are cis-acting DNA elements that mediate epigenetic gene silencing by Polycomb group (PcG) proteins. Here, we report that Pleiohomeotic (PHO) and a multiprotein Polycomb core complex (PCC) bind highly cooperatively to PREs. We identified a conserved sequence motif, named PCC-binding element (PBE), which is required for PcG silencing in vivo. PHO sites and PBEs function as an integrated DNA platform for the synergistic assembly of a repressive PHO/PCC complex. We termed this nucleoprotein complex silenceosome to reflect that the molecular principles underpinning its assemblage are surprisingly similar to those that make an enhanceosome.
Mohd-Sarip
Adone
A
Department of Biochemistry, Centre for Biomedical Genetics, Erasmus University Medical Center, 3000 DR Rotterdam, The Netherlands.
Cléard
Fabienne
F
Mishra
Rakesh K
RK
Karch
François
F
Verrijzer
C Peter
CP
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D044127:Epigenesis, Genetic; D020868:Gene Silencing; D063150:Polycomb Repressive Complex 1; D063146:Polycomb-Group Proteins; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C066770:Pc protein, Drosophila; D063146:Polycomb-Group Proteins; D014157:Transcription Factors; C113295:pho protein, Drosophila; D004247:DNA; D063150:Polycomb Repressive Complex 1