Transcriptome analysis of the murine forelimb and hindlimb autopod.
To gain insight into the coordination of gene expression profiles during forelimb and hindlimb differentiation, a transcriptome analysis of mouse embryonic autopod tissues was performed using Affymetrix Murine Gene Chips (MOE-430). Forty-four transcripts with expression differences higher than 2-fold (T test, P < or = 0.05) were detected between forelimb and hindlimb tissues including 38 new transcripts such as Rdh10, Frzb, Tbx18, and Hip that exhibit differential limb expression. A comparison of gene expression profiles in the forelimb, hindlimb, and brain revealed 24 limb-signature genes whose expression was significantly enriched in limb autopod versus brain tissue (fold change >2, P < or = 0.05). Interestingly, the genes exhibiting enrichment in the developing autopod also segregated into significant fore- and hindlimb-specific clusters (P < or = 0.05) suggesting that by E 12.5, unique gene combinations are being used during the differentiation of each autopod type.
Shou
Siming
S
Research Division, Shriners Hospital for Children, Portland, OR 97239, USA.
Scott
Virginia
V
Reed
Cheryl
C
Hitzemann
Robert
R
Stadler
H Scott
HS
D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002454:Cell Differentiation; D005552:Forelimb / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D020869:Gene Expression Profiling; D006614:Hindlimb / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000378:metabolism*; D013997:Time Factors; D014212:Tretinoin / Q000378:metabolism
D014212:Tretinoin; D012313:RNA