[Study of lipoprotein (a) phenotypes in Ivorian subject].
The objective of this study was to identify the apo (a) phenotypes and to find a correlation between apo (a) isoform size and Lp (a) plasma level in Ivorian subjects. This study involved 30 healthy subjects (11 females and 19 males) aged of 35 +/- 2 years. Lp (a) plasma levels have been determined by technical ELISA, while phenotypes of apo (a) have been identified by agarose high resolution electrophoresis followed by Western blot. The Lp (a) plasma level in our population was 329 +/- 291 mg/l and 30% of our population's Lp (a) plasma levels were above 300 mg/l. 70% of our subjects have homozygous phenotype and 30% have heterozygous phenotype. Three apo (a) isoforms (S2, S3, S4) have been identified in homozygous subjects whereas four apo (a) isoforms (B, S2, S3, S4) have been detected in heterozygous subjects. Their sizes varied from 13 to 33 kringle 4.77% of our subjects had apo (a) isoforms whose sizes were above 22 kringle 4. No correlation has been observed between the size of apo (a) isoforms and the Lp (a) plasma level in homozygous subjects. Our results highlight apo (a) polymorphism in Ivorians. Homozygous phenotypes and large size apo (a) isoforms predominate in this population.
Tiahou
G
G
Laboratoire de biochimie, Hôpital Lapeyronie, Montpellier, France. tiahoug@yahoo.fr
Aveynier
E
E
Djessou
P
P
Sess
D
D
Cristol
J P
JP
D000328:Adult; D001054:Apolipoproteins A / Q000097:blood / Q000235:genetics; D001161:Arteriosclerosis / Q000097:blood; D015153:Blotting, Western; D007560:Cote d'Ivoire; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D017270:Lipoprotein(a) / Q000097:blood* / Q000235:genetics; D008297:Male; D010641:Phenotype; D011110:Polymorphism, Genetic; D020033:Protein Isoforms; D012307:Risk Factors
D001054:Apolipoproteins A; D017270:Lipoprotein(a); D020033:Protein Isoforms