Isolation of three new naturally occurring compounds from the culture of Micromonospora sp. P1068.
Bioassay guided isolation of an antibacterial extract prepared from the fermentation broth of a Micromonospora sp. P1068 led to the isolation of eight compounds identified as (3R) 3,4',7-trihydroxy-isoflavanone (1), 3-hydroxydehydrodaidzein, daidzein (2), 3-methyl-1H-indole-2-carboxylic acid (3), 1H-indole-3-carboxaldehyde (4), 3-(p-hydroxyphenyl)-N-methylpropionamide, N-methylphloretamide (5), phenyl acetic acid (6), 2-hydroxy phenyl acetic acid (7) and 4-hydroxy-5-methoxy-benzoic acid (8). Compounds 1 and 5 were found to be novel chemical entities while 3 was isolated from a natural source for the first time. All compounds were evaluated for their antimicrobial activities against a panel of clinically significant microorganisms. Compound 4 was active against Staphylococcus aureus (MIC, 32 microg/ml), Enterococcus faecium (MIC, 32 microg/ml) and Escherichia coli (MIC, 64 microg/ml).
Gutierrez-Lugo
Maria-Teresa
MT
Department of Pharmacology and Toxicology, Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Arizona, 1703 E, Mabel Street, Tucson, Arizona 85721-0207, USA.
Woldemichael
Girma M
GM
Singh
Maya P
MP
Suarez
Paola A
PA
Maiese
William M
WM
Montenegro
Gloria
G
Timmermann
Barbara N
BN
D001681:Biological Assay; D005285:Fermentation; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D026121:Indole Alkaloids / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008847:Micromonospora / Q000737:chemistry*
D005419:Flavonoids; D026121:Indole Alkaloids