Factors influencing [F-18] 2-fluoro-2-deoxy-D-glucose (F-18 FDG) uptake in melanoma cells: the role of proliferation rate, viability, glucose transporter expression and hexokinase activity.
Using human (SK-MEL 23, SK-MEL 24 and G361) and murine (B16) melanoma cell lines, the coregulatory potential of the uptake of the positron emission tomography (PET) tracer, [Fluorine-18] 2-fluoro-2-deoxy-D-glucose (F-18 FDG) has been investigated in relationship to tumor characteristics. Comparative studies among the four melanoma cell lines demonstrated that the lowest FDG uptake in SK-MEL 24 corresponded strongly to the data for DT (population doubling time) and MTT (tetrazolium salt) cell viability as well as hexokinase (HK) activity, but was not related to the glucose transporter 1 (GLUT 1) expression level. Furthermore, the FDG uptake in each melanoma cell line measured by cell cycle kinetics was significantly positively correlated to both the proliferation index (PI=S/G2M phase fractions) and the cell viability, though with one exception relating to the PI of the lowest FDG uptake cell line, SK-MEL 24. No positive correlation was found between the expression of GLUT 1 and FDG uptake in any individual cell line. However, the HK activities in SK-MEL 23 and 24 showed considerable positive relationships with FDG uptake. Our present study suggests that both the proliferation rate and the cell viability of melanoma cells may be key factors for FDG uptake and that HK activity, rather than GLUT 1 expression, seems to be a major factor.
Yamada
Kiyoshi
K
Department of Internal Medicine 1, Hematology/Oncology, Freiburg University Medical Center, Hugstetter str. 55, 79106 Freiburg i Br., Germany.
Brink
Ingo
I
Bissé
Emmanuel
E
Epting
Thomas
T
Engelhardt
Rupert
R
D000818:Animals; D001682:Biological Availability; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D005947:Glucose / Q000378:metabolism; D006593:Hexokinase / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008544:Melanocytes / Q000187:drug effects* / Q000502:physiology*; D008545:Melanoma / Q000000981:diagnostic imaging / Q000473:pathology; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000032:analysis / Q000378:metabolism*; D049268:Positron-Emission Tomography; D012307:Risk Factors; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014407:Tumor Cells, Cultured
D009004:Monosaccharide Transport Proteins; D019788:Fluorodeoxyglucose F18; D006593:Hexokinase; D005947:Glucose