Direct fluorimetric determination of ascorbic acid by the supramolecular system of AA with beta-cyclodextrin derivative.
We established a simple and selective fluorimetric method for the determination of ascorbic acid (AA) with the novel mono-[6-N(4-carboxy-phenyl)]-beta-cyclodextrin (ACD). The method is based on the fluorescence intensity of ACD decreases as the ACD-AA supramolecular complexes form. The fluorescence intensity is measured at excitation and emission wavelengths of 270 and 352 nm, respectively. Under optimum condition, a linear relationship is obtained between the fluorescence intensity and the concentration of AA in the range of 0.05-8.0 microgml(-1). The detection limit is 0.012 microgml(-1). The method has been applied to the direct analysis of AA in real samples with satisfactory results.
Wang
Lun
L
College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. zhangli322@etang.com
Zhang
Li
L
She
Shike
S
Gao
Feng
F
D000042:Absorption; D001205:Ascorbic Acid / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry*; D002138:Calibration; D003683:Dehydroascorbic Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000737:chemistry*
D047392:beta-Cyclodextrins; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid