Determination of nitrofuran metabolites in poultry muscle and eggs by liquid chromatography-tandem mass spectrometry.
The use of nitrofurans in food-producing animals has been banned in EU. Detection of the protein-bound nitrofuran metabolites is the best approach to evaluate their utilization. A fast, sensitive and reliable LC-MS-MS method is presented to analyze simultaneously the metabolites of four commonly used nitrofuran drugs, furazolidone, furaltadone, nitrofurazone and nitrofurantoin. The sample clean up was performed by a single liquid-liquid extraction step, after a hydrolysis and derivatisation process. Separation of the molecules was performed by liquid chromatography in a C18 column (100 mmx2.1 mm, 4 microm) at room temperature. The quantitative and confirmatory determination of these metabolites was performed by multiple reactions monitoring (MRM). Limits of quantification of 0.5 ngg(-1) were achieved and the total analysis was accomplished in 5 min. This protocol has been applied to identify contaminated samples of poultry muscle and egg products.
Finzi
Jane Kelly
JK
Galeno Research Unit, 1301 Latino Coelho, Parque Taquaral, Campinas, SP 13087-10, Brazil.
Donato
Jose Luiz
JL
Sucupira
Mauro
M
De Nucci
Gilberto
G
D000818:Animals; D002138:Calibration; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat / Q000032:analysis*; D009581:Nitrofurans / Q000032:analysis* / Q000378:metabolism; D015203:Reproducibility of Results
D009581:Nitrofurans