Iteratively acting glycosyltransferases involved in the hexasaccharide biosynthesis of landomycin A.
Detailed studies on the biosynthesis of the hexasaccharide side chain of landomycin A, produced by S. cyanogenus S136, revealed the function of each glycosyltransferase gene of the biosynthetic gene cluster. Analyses of generated mutants as well as feeding experiments allowed us to determine that LanGT2 and LanGT3 catalyze the attachment of one sugar, whereas LanGT1 and LanGT4 attach two sugars during landomycin A biosynthesis. The generation of a lanZ2 deletion mutant provided evidence that LanZ2 is controlling the elongation of the saccharide side chain.
Luzhetskyy
Andriy
A
Albert-Ludwigs-Universität, Institut für Pharmazeutische Wissenschaften, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
Fedoryshyn
Marta
M
Dürr
Clemens
C
Taguchi
Takaaki
T
Novikov
Volodymyr
V
Bechthold
Andreas
A
D000617:Aminoglycosides / Q000096:biosynthesis* / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002384:Catalysis; D008401:Gas Chromatography-Mass Spectrometry; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D011134:Polysaccharides; C064648:landomycin A; D016695:Glycosyltransferases