Oxidative and nitrosative responses of the chicken macrophage cell line MQ-NCSU to experimental Salmonella infection.
Phagocytes limit replication or kill ingested organisms by producing toxic reactive oxygen and nitrogen species via NADPH oxidase and inducible nitric oxide synthase (iNOS). The present experiments were to investigate the production and the possible roles of superoxide, hydrogen peroxide (H2O2) and nitric oxide (NO) in the MQ-NCSU chicken macrophage cell line infected with Salmonella in vitro. After infection, intracellular Salmonella viable counts remained constant until 24 h post infection (PI) and started to decline from 48 h PI. Infection of cells with S. Typhimurium, S. Enteritidis and S. Gallinarum, as well as exposure to S. Enteritidis LPS induced low, but significant concentrations of superoxide 1 to 2 h PI, as determined by reduction of ferricytochrome c. There was no difference in superoxide production in infected cells and control cells after 4 h. Increased H2O2 was observed from cells infected with all the different Salmonella species between 2 and 3 h of infection. Nitrite was always greater in infected cells compared to uninfected cells at all times. However, Salmonella was not completely eliminated from the cells though these cells are capable of eliciting a noticeable oxidative burst response and great nitrosative responses, indicating that a strong oxidative burst (and other mechanism/s) is essential for the elimination of intracellular Salmonella.
Withanage
G S K
GS
Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, England. sam.withanage@spcorp.com
Mastroeni
P
P
Brooks
H J
HJ
Maskell
D J
DJ
McConnell
I
I
D000818:Animals; D002460:Cell Line; D002645:Chickens / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress; D019779:Salmonella enterica / Q000276:immunology / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
D013481:Superoxides; D009569:Nitric Oxide; D006861:Hydrogen Peroxide