Studies on the metabolism and toxicological detection of the new designer drug 4'-methyl-alpha-pyrrolidinobutyrophenone (MPBP) in rat urine using gas chromatography-mass spectrometry.
The aim of the presented study was to identify the metabolites of the new designer drug 4'-methyl-alpha-pyrrolidinobutyrophenone (MPBP) in rat urine using GC-MS techniques. After enzymatic hydrolysis, extraction and various derivatizations, seven metabolites of MPBP could be identified suggesting the following metabolic steps: oxidation of the 4'-methyl group to the corresponding alcohol and further oxidation to the respective carboxy compound, hydroxylation of the pyrrolidine ring followed by dehydrogenation to the corresponding lactam or reduction of the keto group to the 1-dihydro compound. A previously published GC-MS-based screening procedure for pyrrolidinophenones involving enzymatic hydrolysis and mixed-mode solid-phase extraction of urine samples allowed detection of MPBP metabolites. Assuming similar metabolism and dosages in humans, an intake of MPBP should be detectable via its metabolites in urine.
Peters
Frank T
FT
Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Saarland, D-66421 Homburg (Saar), Germany. frank.peters@uniklinikum-saarland.de
Meyer
Markus R
MR
Fritschi
Giselher
G
Maurer
Hans H
HH
D000818:Animals; D002090:Butyrophenones / Q000378:metabolism / Q000633:toxicity / Q000652:urine*; D015198:Designer Drugs / Q000032:analysis* / Q000633:toxicity; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D011759:Pyrrolidines / Q000378:metabolism / Q000633:toxicity / Q000652:urine*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
C504661:4'-methyl-alpha-pyrrolidinobutyrophenone; D002090:Butyrophenones; D015198:Designer Drugs; D011759:Pyrrolidines