title: Acetoacetyl-CoA synthetase gene is abundant in rat adipose, and related with fatty acid synthesis in mature adipocytes.
abstract: Acetoacetyl-CoA synthetase (AACS, acetoacetate-CoA ligase, EC 6.2.1.16) is a novel cytosolic ketone body (acetoacetate)-specific ligase, the physiological role of which remains to be elucidated. We examined the expression profiles of AACS mRNA in adult rat tissues, finding that it was particularly abundant in male subcutaneous white adipose tissue after weaning. In white adipose tissue, AACS mRNA was preferentially detected in mature adipocytes but not in preadipocytes. The AACS mRNA expression in primary preadipocytes increased during the adipocyte differentiation. These expression profiles were similar to that of acetyl-CoA carboxylase-1, but not like to that of 3-hydroxy-3-methylglutaryl-CoA reductase. These results suggest that AACS in adipose tissue plays an important role in utilizing ketone body for the fatty acid-synthesis during adipose tissue development.
authors.affiliation: Department of Health Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan. ymskmshr@hoshi.ac.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Shinya
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Kensuke
authors.initials: K
authors.lastname: Hidai
authors.forename: Yuji
authors.initials: Y
authors.lastname: Saitoh
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Fukui
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D005227:Fatty Acids; D012333:RNA, Messenger; D003066:Coenzyme A Ligases; C023747:acetoacetyl-CoA synthetase