A genome wide quantitative trait linkage analysis for serum lipids in type 2 diabetes in an African population.
Lipid abnormalities are strongly linked with coronary heart disease and are common in type 2 diabetes. However, little is known about the genetic determinants of serum lipids in African populations. An autosomal genome scan was performed for linkage to five plasma lipid phenotypes (total cholesterol, triglycerides (TG), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C) and VLDL-cholesterol (VLDL-C)) in the Africa-America Diabetes Mellitus (AADM) study. Two hundred and ninety-five affected sibling pairs with type 2 diabetes mellitus enrolled from Ghana and Nigeria were genotyped for 390 microsatellite markers with an average inter-marker distance of 9cM. Multipoint variance components linkage analysis showed that HDL-C had a LOD score of 4.34 near marker D7S3061 and 3.00 near marker D7S513. Some clustering of linkage evidence to several lipid phenotypes was observed on chromosomes 5 (LDL-C, total cholesterol, VLDL-C), chromosome 7 (HDL-C, TG) and chromosome 19 (total cholesterol, LDL-C, TG). Principal component analysis of the five phenotypes yielded two factors, one (TG, HDL-C and VLDL) of which was linked to QTLs on chromosomes 2, 5 and 7, while the other (total cholesterol and LDL-C) was linked to a different set of QTLs on chromosomes 2, 5 and 18. Several of these regions have been reported to be linked to lipids in other studies. Follow up investigations are warranted in view of the central role serum lipids play in the aetiopathogenesis of cardiovascular disease.
Adeyemo
Adebowale A
AA
National Human Genome Center, Howard University, Genetic Epidemiology Unit, College of Medicine, 2216 6th Street, NW, Washington, DC 20059, USA.
Johnson
Thomas
T
Acheampong
Joseph
J
Oli
Johnnie
J
Okafor
Godfrey
G
Amoah
Albert
A
Owusu
Samuel
S
Agyenim-Boateng
Kofi
K
Eghan
Benjamin A
BA
Abbiyesuku
Fayeofori
F
Fasanmade
Olufemi
O
Rufus
Theresa
T
Doumatey
Ayo
A
Chen
Guanjie
G
Zhou
Jie
J
Chen
Yuanxiu
Y
Furbert-Harris
Paulette
P
Dunston
Georgia
G
Collins
Francis
F
Rotimi
Charles
C
D000328:Adult; D001741:Black or African American / Q000235:genetics*; D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000208:ethnology / Q000235:genetics*; D023281:Genomics; D005838:Genotype; D005869:Ghana / Q000208:ethnology; D006801:Humans; D008126:Lod Score; D008875:Middle Aged; D009549:Nigeria / Q000208:ethnology; D040641:Quantitative Trait Loci; D014481:United States / Q000453:epidemiology
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D002784:Cholesterol