One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on beta-cyclodextrin substituted agarose gel media.
An oligomerized beta-cyclodextrin ligand coupled to brominated allyl-group substituted Sepharose HP has been used for the one-step purification of polyphenolic epigallocatechin gallate (EGCG), an important antioxidant, by isocratic hydrogen bond adsorption chromatography. With a sample load of 1.33 mg crude green tea polyphenolic extract per ml column packing and with water/ethanol/acetonitrile (57/30/13, v/v) as the optimum mobile phase, an EGCG purity of about 98% with a recovery of approximate 73% could be achieved by proper peak cutting. After about 10 sample applications, the column performance started to deteriorate but could be regenerated to its original function by cleaning with 0.35 M NaOH.
Xu
Jun
J
Department of Bioengineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Zhang
Guifeng
G
Tan
Tianwei
T
Janson
Jan-Christer
JC
D002392:Catechin / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006860:Hydrogen Bonding; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000032:analysis; D012685:Sepharose / Q000737:chemistry; D013662:Tea / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry
D010936:Plant Extracts; D013662:Tea; D047392:beta-Cyclodextrins; D002392:Catechin; D012685:Sepharose; C045651:epigallocatechin gallate; C031215:betadex