title: Virulence comparisons of Aspergillus nidulans mutants are confounded by the inflammatory response of p47phox-/- mice.
abstract: While investigating the requirement for phagosomal alkalinization in the host defense against pulmonary aspergillosis, we observed high morbidity of p47(phox)(-/-) mice infected with pH-insensitive Aspergillus nidulans mutants despite a paucity of fungal growth. Fatal infection also resulted from a normally avirulent p-aminobenzoate auxotroph. This demonstrates that p47(phox)(-/-) murine immunity contributes significantly to A. nidulans lethality. These data have wider implications for microbial virulence studies with p47(phox)(-/-) mice.
authors.affiliation: Department of Infectious Diseases, Imperial College London, Du Cane Road, London W12 0NN, United Kingdom. e.bignell@imperial.ac.uk
authors.forename: Elaine
authors.initials: E
authors.lastname: Bignell
authors.forename: Susana
authors.initials: S
authors.lastname: Negrete-Urtasun
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Calcagno
authors.forename: Herbert N
authors.initials: HN
authors.lastname: Arst
authors.forename: Tom
authors.initials: T
authors.lastname: Rogers
authors.forename: Ken
authors.initials: K
authors.lastname: Haynes
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D001233:Aspergillus nidulans / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D006863:Hydrogen-Ion Concentration; D008172:Lung Diseases, Fungal / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D051379:Mice / Q000276:immunology* / Q000378:metabolism; D009154:Mutation; D019255:NADPH Oxidases; D010750:Phosphoproteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014774:Virulence
chemical_list: D010750:Phosphoproteins; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1