Probing of specific binding of synthetic sulfonylurea with the insulinoma cell line MIN6.
To overcome the limitation of conventional sulfonylurea (SU) for investigation of biological mechanisms related to KATP channels, a hypoglycemic sulfonylurea (SU) was conjugated with a non-reducing glucose bearing polystyrene (PS) derivative to provide enhanced interaction with an insulinoma cell line (MIN6). The specific interaction between the SU (K+ channel closer)-conjugated copolymer and MIN6 cells was confirmed by confocal laser microscopic images using rhodamine B isothiocyanate (RITC)-labeled SU-conjugated polymer, which revealed the specific interaction between SU-conjugated polymer and MIN6 cells. Moreover, the location of labeled polymer and the site of Ca2+ ion mobilization obtained from the same MIN6 cells were identical. Based on the specificity and insulinotropic activity, the SU-conjugated polymer is expected to be useful tool for the study of biological mechanisms of KATP channels.
Park
Keun-Hong
KH
College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute 605, Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Korea.
Akaike
Toshihiro
T
D002118:Calcium / Q000378:metabolism; D003981:Diazoxide / Q000494:pharmacology; D005905:Glyburide / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000378:metabolism / Q000494:pharmacology*; D007340:Insulinoma / Q000378:metabolism* / Q000473:pathology; D018613:Microscopy, Confocal; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism; D013453:Sulfonylurea Compounds / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D007004:Hypoglycemic Agents; D015221:Potassium Channels; D013453:Sulfonylurea Compounds; D003981:Diazoxide; D005905:Glyburide; D002118:Calcium