title: Quantitative determination of endogenous sorbitol and fructose in human erythrocytes by atmospheric-pressure chemical ionization LC tandem mass spectrometry.
abstract: Evaluation of different extraction methods for quantification of endogenous sorbitol and fructose in human red blood cells (RBCs) and matrix effects in ESI and APCI showed that protein-precipitation followed by mixed-mode solid-phase extraction was more effective extraction method and APCI more effective ionization method. Then the LC/APCI-MS/MS method was fully validated and successfully applied to analysis of clinical RBC samples. The concentrations of endogenous sorbitol and fructose were determined using calibration curves employing sorbitiol-13C6 and fructose-13C6 as surrogate analytes. The method has provided excellent intra- and inter-assay precision and accuracy with a linear range of 50.0-10,000 ng/mL (correlation coefficient >0.999) for sorbitol-13C6 and 250-50000 ng/mL (correlation coefficient >0.999) for fructose-13C6 in human RBCs.
authors.affiliation: Tandem Labs, A Division of NWT Inc., Salt Lake City, UT, USA. hairui.liang@us.crl.com
authors.forename: H R
authors.initials: HR
authors.lastname: Liang
authors.forename: T
authors.initials: T
authors.lastname: Takagaki
authors.forename: R L
authors.initials: RL
authors.lastname: Foltz
authors.forename: P
authors.initials: P
authors.lastname: Bennett
mesh_terms: D001274:Atmospheric Pressure; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry*; D005632:Fructose / Q000097:blood* / Q000737:chemistry / Q000592:standards; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D013012:Sorbitol / Q000097:blood* / Q000737:chemistry / Q000592:standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D013237:Stereoisomerism
chemical_list: D005632:Fructose; D013012:Sorbitol