title: [The photodynamic therapy effect of a new cationic porphyrin on human laryngeal cancer Hep-2 cell in vitro].
abstract: OBJECTIVE
  To investigate the photodynamic therapy (PDT) effect of a new kind of cationic porphyrin on human laryngeal cancer Hep-2 cell in vitro.
  
  
  METHODS
  A new cationic porphyrin (5-(4-methoxycarbonylphenyl)-10, 15, 20-tris(4-N-methylpyridiniumyl)porphyrin triiodide ) was synthesized. The Hep-2 cells were treated with three different concentrations of porphyrin (10(-7) mol/L, 10(-6) mol/L, 10(-5) mol/L) followed by irradiation of different visible light (0, 0.5, 1.0, 1.5, 2.0 h). MTT and TUNEL were applied to measure the inhibitory effect of porphyrin on the Hep-2 cells growth, inhibitory rate and induced apoptosis rate.
  
  
  RESULTS
  Under definite condition, the cantionic porphyrin concentrations had a significant inhibitory effect and induced apoptosis effect. The effect depended greatly on the doses of the concentration and the time of irradiation of light. At the highest concentration of porphyrin (10(-5) mol/L), the growth inhibitory rate were 1.18%, 35.21%, 58.59%, 70.42%, 84.34% respectively and the apoptosis rate were 3.30% +/- 0.29%, 29.41% +/- 2.12%, 32.30% +/- 2.26%, 34.52% +/- 3.11% in different visible light (0.05, 1.0, 1.5, 2.0 h), respectively. Under high irradiation energy, it can causesome Hep-2 cells death directly.
  
  
  CONCLUSIONS
  Porphyrin has a distinctive killing effect on Hep-2 cells and correlated with the doses and the irradiation time.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery,Renmin Hospital,Wuhan University, Wuhan, 430060, China. yfb85680076@sina.com
authors.forename: Fubo
authors.initials: F
authors.lastname: Yan
authors.forename: Zezhang
authors.initials: Z
authors.lastname: Tao
authors.forename: Tao
authors.initials: T
authors.lastname: Jia
authors.forename: Bokui
authors.initials: B
authors.lastname: Xiao
authors.forename: Huaming
authors.initials: H
authors.lastname: Chi
authors.forename: Shiming
authors.initials: S
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010778:Photochemotherapy; D011166:Porphyrins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D011166:Porphyrins