Isolation and identification of Sphingomonas sp. that yields tert-octylphenol monoethoxylate under aerobic conditions.
Topsoil samples were collected from eight golf courses in Yamaguchi Prefecture, Japan, and enrichment cultures were carried out with a basal-salt medium containing 0.2% 4-tert-octylphenol polyethoxylate (OPPEO) as sole carbon source. OPPEO-degrading activity was detected in one of the samples, from which a strain of OPPEO-degrading bacterium was isolated. The isolated bacterium grew on a nutritionally enriched medium (NE medium) containing 0.2% OPPEO as sole carbon source, and accumulated 4-tert-octylphenol diethoxylate (OP2EO) (63%), 4-tert-octylphenol triethoxylate (OP3EO) (14%), and 4-tert-octylphenol monoethoxylate (OP1EO) (2%) after 7 d cultivation under aerobic conditions. The addition of clay mineral (vermiculite) to the medium accelerated the degradation of OP2EO (40%) and OP3EO (4%) to OP1EO (23%). This is the first report about bacteria that can degrade OPPEO to OP1EO under aerobic conditions. The strain was identified as Sphingomonas macrogoltabidus, based on the homology of a 16S rDNA sequence.
Nishio
Eriko
E
Department of Environmental Chemistry, Kyushu Kyoritsu University, Kitakyushu 807-8585, Japan. eriko@kyukyo-u.ac.jp
Yoshikawa
Hiromichi
H
Wakayama
Manabu
M
Tamura
Hiroto
H
Morita
Shiro
S
Tomita
Yoshifumi
Y
D000332:Aerobiosis; D000538:Aluminum Silicates / Q000494:pharmacology; D001673:Biodegradation, Environmental / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000235:genetics; D005019:Ethyl Ethers / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism; D020578:Sphingomonas / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D000538:Aluminum Silicates; D004269:DNA, Bacterial; D005019:Ethyl Ethers; D010636:Phenols; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C003760:vermiculite; C105260:4-tert-octylphenol