title: [Antigen analysis of Angiostrongylus cantonensis in different developmental stages].
abstract: OBJECTIVE
  To analyze the difference among antigens of Angiostrongylus cantonensis in different developmental stages and identify dominant diagnostic antigen for angiostrongyliasis.
  
  
  METHODS
  Antigens of A. cantonensis in different developmental stages were analyzed by SDS-PAGE and immunoblot.
  
  
  RESULTS
  The protein bands of all developmental stages were similar on SDS-PAGE. The Mr 40000, 50000, 66000 and 80000 antigens reacted not only with the sera of rats infected by A. cantonensis but also with the sera of normal rats. The Mr 104000 antigen could be discerned by sera of rats infected with A. cantonensis for 2 weeks. The Mr 32000 antigen could be recognized by sera of rats 2 weeks after infection, and the reaction became stronger with the infection continued.
  
  
  CONCLUSIONS
  The Mr 40000, 50000, 66000 and 80000 antigens might result in the unspecific reaction in the immunodiagnosis of angiostrongyliasis using the crude antigen of A. cantonensis. The Mr 104000 of larva, Mr 33000 of adult females and Mr 32000 of the worms might be used as candidate antigens in early diagnosis and epidemiological survey of angiostrongyliasis.
authors.affiliation: Department of Parasitology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Chen
authors.forename: Hao-xian
authors.initials: HX
authors.lastname: Shen
authors.forename: Hong-juan
authors.initials: HJ
authors.lastname: Peng
authors.forename: Xing-cun
authors.initials: XC
authors.lastname: Zhao
mesh_terms: D017185:Angiostrongylus cantonensis / Q000254:growth & development / Q000276:immunology*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D051381:Rats; D017207:Rats, Sprague-Dawley; D017206:Strongylida Infections / Q000175:diagnosis / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth