title: Soluble CD44v6 is not a sensitive tumor marker in patients with head and neck squamous cell cancer.
abstract: BACKGROUND
  In some epithelial tumors, isoforms of CD44 are overexpressed and soluble isoforms detectable in serum samples are elevated. In squamous cell cancer of the head and neck (SCCHN) the alteration of CD44 isoforms could be associated with poor prognosis. A comprehensive study was undertaken to examine the value of CD44v6 as a tumor marker for SCCHN.
  
  
  METHODS
  Serum samples of SCCHN and healthy smokers were analyzed for soluble CD44v6 by ELISA. The expression of CD44 isoforms was determined by immunohistochemical staining of healthy and dysplastic tissue.
  
  
  RESULTS
  There was no significant difference between the serum levels of sCD44v6 in SCCHN and healthy smokers. Nor was there a correlation between the serum level of sCD44v6 and UICC stage, TNM stage or histological grading. In tissue of primary SCCHN, expression of CD44v6 was found as a strong, specific staining of the lower epithelial layers. Similar amounts of CD44v6-positive-labelled tumor cells were found in invasive carcinoma.
  
  
  CONCLUSIONS
  Soluble CD44v6 is not a valuable tumor marker for SCCHN since the soluble form appears to be present in healthy smokers and does not reflect the stage of the disease.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Ludwig-Maximilians-University of Munich, Marchioninistrasse 15, 81377 Munich, Germany. Michaela.Andratschke@med.uni-muenchen.de
authors.forename: Michaela
authors.initials: M
authors.lastname: Andratschke
authors.forename: Sarita
authors.initials: S
authors.lastname: Chaubal
authors.forename: Christof
authors.initials: C
authors.lastname: Pauli
authors.forename: Brigitte
authors.initials: B
authors.lastname: Mack
authors.forename: Hjalmar
authors.initials: H
authors.lastname: Hagedorn
authors.forename: Barbara
authors.initials: B
authors.lastname: Wollenberg
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000097:blood*; D006258:Head and Neck Neoplasms / Q000097:blood* / Q000473:pathology; D006801:Humans; D018960:Hyaluronan Receptors / Q000097:blood*; D007150:Immunohistochemistry; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; C112697:CD44v6 antigen; D006023:Glycoproteins; D018960:Hyaluronan Receptors