Molecular characterization of Agrotis segetum nucleopolyhedrovirus from Poland.
The turnip moth, Agrotis segetum (Lepidoptera, Noctuidae), is an important pest insect in Europe, Asia, and Africa. We have genetically characterized and classified a nucleopolyhedrovirus isolated from A. segetum larvae in Poland (AgseNPV-P). The restriction pattern of AgseNPV-P was distinct from an isolate from England/France (AgseNPV-UK and AgseNPV-F). Sequence analysis of three conserved baculovirus genes, polyhedrin, lef-8 and pif-2, revealed that AgseNPV-P differs substantially from the already described NPVs isolated from A. segetum and possibly represents a new NPV species. Phylogenetic analysis placed AgseNPV-P among group II NPVs and showed the closest relationship to Agrotis ipsilon (Agip) NPV and Spodoptera exigua (Se) MNPV.
Jakubowska
Agata
A
Laboratory of Virology, Wageningen University, Binnenhaven 11, Wageningen, 6709 PD, The Netherlands.
van Oers
Monique M
MM
Ziemnicka
Jadwiga
J
Lipa
Jerzy J
JJ
Vlak
Just M
JM
D000818:Animals; D020869:Gene Expression Profiling; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D010802:Phylogeny; D011044:Poland