Hypocretin/orexin-containing neurons are produced in one sharp peak in the developing ventral diencephalon.
The birth date of hypocretin-containing neurons was analysed using the bromodeoxyuridine method in the rat. The results indicate that these neurons are generated between embryonic days 11 (E11) and E14, with a sharp peak on E12. This spatiotemporal pattern of genesis contrasts with that of the co-distributed neurons producing the melanin-concentrating hormone in the lateral hypothalamic area, which have been described as generated in one large peak from E10 to E16. These observations may be linked to the relative distribution area of both populations.
Amiot
C
C
Université de Franche-Comté, EA 3922, Faculté de Médecine et de Pharmacie, 19 rue Ambroise Paré, 25041 Besançon cedex 3, France.
Brischoux
F
F
Colard
C
C
La Roche
A
A
Fellmann
D
D
Risold
P Y
PY
D000367:Age Factors; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D004027:Diencephalon / Q000166:cytology* / Q000196:embryology; D004622:Embryo, Mammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007026:Hypothalamic Area, Lateral / Q000196:embryology / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D008543:Melanins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D000068797:Orexins; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
D047908:Intracellular Signaling Peptides and Proteins; D008543:Melanins; D009479:Neuropeptides; D000068797:Orexins; D001973:Bromodeoxyuridine