[DNA/MVA combined immunization: antibody response to Plasmodium falciparum merozoite surface protein 1 in mice].
OBJECTIVE
To explore the effect of DNA/MVA combined immunization in enhancing antibody response to MSP1.


METHODS
DNA vaccine and recombined MVA were constructed based on synthesized MSP1 gene (3D7). BALB/c mice were primed with DNA solely or together with GM-CSF expressing plasmid and then boosted with rMVA/ 190. Serum IgG and subtype IgG1 and IgG2a were assayed by ELISA. All mice were challenged with allelic replaced Plasmodium berghei.


RESULTS
Antibodies to MSP1-190 were detected after DNA immunization with an end-point dilution titer of 1:2500. When GM-CSF plasmid was added, the antibody end-point dilution titer reached 1:11150, with an increase of 53 and 10 times respectively after MVA boosting. Among them anti-19000 antibodies were prominent, 1/4-1/3 of total IgG in serum. However, when the mice were challenged with Pb-PfM19 no prolonged survival was observed (P>0.05).


CONCLUSIONS
High titer antibodies can be elicited in mice by using codon optimized MSPI gene and DNA/MVA combined immunization. The specificity and protection of these antibodies is being further investigated.
Li
Shu-Mei
SM
Department of Etiology, The Fourth Military Medical University, Xi'an 710032, China.
Li
Xun
X
Xue
Cai-Fang
CF
Miao
Jun
J
Lei
Jun-Chuan
JC
Liu
Zhong-Xiang
ZX
Wang
Xian-Feng
XF
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood*; D020066:Merozoite Surface Protein 1 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000276:immunology*; D017778:Vaccines, Combined / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D020066:Merozoite Surface Protein 1; D017778:Vaccines, Combined; D019444:Vaccines, DNA