Brown adipose tissue mitochondrial subpopulations show different morphological and thermogenic characteristics.
Rat brown adipose tissue mitochondrial subpopulations-isolated by differential centrifugation at 1000, 3000 and 8000g, giving the heavy, medium and light mitochondria-were characterized. Thus, contamination by non-mitochondrial subcellular components, morphological features, respiratory chain and antioxidant enzyme activities, both uncoupling protein 1 and mitochondrial protein content, mitochondrial DNA levels and mitochondrial integrity were measured. Results indicate that mitochondrial fractions showed important differences in the morphological, thermogenic and antioxidant properties. All the parameters studied were always higher in heavy mitochondria, which is indicative of a greater mitochondrial differentiation state.
Justo
Roberto
R
Departament de Biologia Fonamental i Ciències de la Salut, Institut Universitari d'lnvestigacióen Ciències de la Salut (IUNICS), Universitat de les Illes Balears, E-07122 Palma de Mallorca, Spain.
Oliver
Jordi
J
Gianotti
Magdalena
M
D002001:Adipose Tissue, Brown / Q000378:metabolism* / Q000648:ultrastructure*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001483:Base Sequence; D002458:Cell Fractionation; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D008854:Microscopy, Electron; D008928:Mitochondria / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure*; D024101:Mitochondrial Proteins / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D022722:Thermogenesis
D000975:Antioxidants; D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins