A system for inducible gene expression in retinal ganglion cells.
OBJECTIVE
To develop a system for inducible gene expression in retinal ganglion cells, Thy1 and ckit promoters were used to direct expression of a second-generation reverse tetracycline transactivator (rtTA2S-M2).


METHODS
Transgenic mice were generated that harbor rtTA2S-M2 under the control of either the Thy1 or ckit promoter. These animals were crossed with mice transgenic for the LacZ gene downstream of a cassette of tet operator (TRE) binding sites. Induction of the LacZ reporter gene in vivo after either oral or subcutaneous doxycycline administration and in vitro in cultured retinal cells was assessed. To examine induction of a secreted protein, expression of pigment epithelium-derived factor (PEDF) in mice harboring Thy1-rtTA and TRE-PEDF constructs was quantified.


RESULTS
Five of seven Thy1-rtTA lines showed induction with subcutaneous doxycycline: maximum induction in one line (Thy1-C), moderate in one line (Thy1-F), and minimal in three lines. There was no detectable retinal LacZ expression in the ckit-rtTA lines, despite expression of the ckit-rtTA transgene at the RNA level. In Thy1-rtTA lines, LacZ reporter expression as measured by X-gal staining was evenly dispersed throughout all quadrants of the retina, present in a subpopulation of retinal ganglion cell (RGC) bodies, RGC axons projecting through the retina and optic nerve, and some cells in the inner nuclear layer. Immunostaining for beta-galactosidase demonstrated more uniform expression in RGCs and cells of the inner aspect of the inner nuclear layer, which, by double staining with anti-beta-galactosidase and anti-calretinin antibodies, were consistent with amacrine cells. More than 95% of Thy-1 antigen-positive cells in the retina expressed the induced transgene. Subcutaneous doxycycline resulted in a more robust induction of LacZ than did oral administration. In vitro, the number of cells induced in culture increased in a dose-dependent manner, with maximum expression at 10 microg/mL at a level 3.4-fold over background. Thy1-rtTA/TRE-PEDF mice treated with doxycycline had 1000-fold induction in their retinal PEDF expression in comparison with nontransgenic mice and 600-fold induction over noninduced Thy1-rtTA/TRE-PEDF mice.


CONCLUSIONS
A transgenic system for inducible RGC expression has been developed that demonstrates minimal leakiness and significant induction with doxycycline. This system will be useful for several applications.
Kerrison
John B
JB
Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. jkerris1@jhmi.edu
Duh
Elia J
EJ
Yu
Yilin
Y
Otteson
Deborah C
DC
Zack
Donald J
DJ
D000818:Animals; D004318:Doxycycline; D005136:Eye Proteins / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D005838:Genotype; D007763:Lac Operon / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000378:metabolism*; D015843:Serpins / Q000096:biosynthesis*; D013754:Tetracyclines / Q000494:pharmacology; D018800:Thy-1 Antigens / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics; D015533:Transcriptional Activation; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism; D000099174:Pigment Epithelium-Derived Factor
D004318:Doxycycline; D005136:Eye Proteins; D009414:Nerve Growth Factors; D019009:Proto-Oncogene Proteins c-kit; D015843:Serpins; D013754:Tetracyclines; D018800:Thy-1 Antigens; D015534:Trans-Activators; D001616:beta-Galactosidase; D000099174:Pigment Epithelium-Derived Factor