Hemolysin chrysolysin from Penicillium chrysogenum promotes inflammatory response.
Some strains of Penicillium chrysogenum produce a proteinaceous hemolysin, chrysolysinTM, when incubated on sheep's blood agar at 37 degrees C but not at 23 degrees C. However, 92% (11/12) of the indoor air isolates produced hemolysis but only 43% (3/7) of the non-indoor air isolates did so. Chrysolysin is an aggregating protein composed of approximately 2kDa monomers, contains one cysteine amino acid, and has an isoelectric point of 4.85. Treatment of murine macrophage cell line RAW 264.7 with purified chrysolysin caused statistically significant (T-test, p < 0.05) increased production of macrophage inflammatory protein-2 (MIP-2) in a dose dependent manner after 6 h treatment. This suggests that chrysolysin might act to promote the host's inflammatory response after P. chrysogenum exposures.
Donohue
Maura
M
National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, OH, USA.
Chung
Yongjoo
Y
Magnuson
Matthew L
ML
Ward
Marsha
M
Selgrade
Mary Jane
MJ
Vesper
Stephen
S
D000818:Animals; D002460:Cell Line; D054426:Chemokine CXCL2; D005656:Fungal Proteins / Q000302:isolation & purification / Q000633:toxicity*; D006460:Hemolysin Proteins / Q000302:isolation & purification / Q000633:toxicity*; D006461:Hemolysis; D007249:Inflammation; D051379:Mice; D015846:Monokines / Q000096:biosynthesis*; D010408:Penicillium chrysogenum / Q000737:chemistry* / Q000145:classification; D013696:Temperature
D054426:Chemokine CXCL2; C506186:Chrysolysin, Penicillium chrysogenum; D005656:Fungal Proteins; D006460:Hemolysin Proteins; D015846:Monokines