title: LC-NMR coupling technology: recent advancements and applications in natural products analysis.
abstract: An overview of recent advances in nuclear magnetic resonance (NMR) coupled with separation technologies and their application in natural product analysis is given and discussed. The different modes of LC-NMR operation are described, as well as how technical improvements assist in establishing LC-NMR as an important tool in the analysis of plant-derived compounds. On-flow, stopped-flow and loop-storage procedures are mentioned, together with the new LC-SPE-NMR configuration. The implementation of mass spectrometry in LC-NMR is also useful on account of the molecular weight and fragmentation information that it provides, especially when new plant species are studied. Cryogenic technology and capillary LC-NMR are the other important recent developments. Since the plant kingdom is endless in producing potential drug candidates, development and optimization of LC-NMR techniques convert the study of natural products to a less-time-consuming task, speeding up identification.
authors.affiliation: NMR Center, Department of Chemistry, University of Ioannina, Greece. vexarc@cc.uoi.gr
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Exarchou
authors.forename: Manfred
authors.initials: M
authors.lastname: Krucker
authors.forename: Teris A
authors.initials: TA
authors.lastname: van Beek
authors.forename: Jacques
authors.initials: J
authors.lastname: Vervoort
authors.forename: Ioannis P
authors.initials: IP
authors.lastname: Gerothanassis
authors.forename: Klaus
authors.initials: K
authors.lastname: Albert
mesh_terms: D045784:Abietanes / Q000032:analysis; D001688:Biological Products / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis; D053630:Depsides; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008832:Microchemistry / Q000639:trends; D010616:Phenanthrenes / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000097123:Rosmarinic Acid
chemical_list: D045784:Abietanes; D001688:Biological Products; D002934:Cinnamates; D053630:Depsides; D010616:Phenanthrenes; D010936:Plant Extracts; D000097123:Rosmarinic Acid; C068623:carnosol; C018381:salvin