[Inducement and proliferation of MSCs in vitro and preliminary study on interface between MSCs and metal materials].
OBJECTIVE
The aims of the present study were to assess the characteristics of MSCs which were induced and proliferated in vitro, then to learn the interfaces between them and metal materials.


METHODS
Adult dogs were selected as experimental animals, the MSCs (marrow stem cells) were aspirated from the femur, which were induced to the phenotype of OB (osteoblast), then to proliferated in vitro with metal materials. Using biochemical tests, phase contrast microscopy, transmit electromicroscope, scanning electromicroscope to detect MSCs and the image of interface.


RESULTS
MSCs had powerful osteogenic phenotype when they were induced in vitro, and they also attached to the metal materials. And there were more cells which attached on tensile stress area than other areas.


CONCLUSIONS
The MSCs induced in vitro could be used as source of osteoblast. The affects of air--abrasion with alumina are not significant, but they have relation to the extent of strain and property of stress.
Yu
Chun-Hua
CH
Dept. of Prosthodontics, The Ninth People's Hospital Affiliated to Shanghai Second Medical University, Shanghai 200011, China.
Zhang
Bao-Wei
BW
Zhang
Xiu-Yin
XY
Gao
Jian-Xin
JX
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004285:Dogs; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells; D008670:Metals; D010006:Osteoblasts
D008670:Metals