title: Protective effect of nordihydroguaiaretic acid (NDGA) against norgestrel induced genotoxic damage.
abstract: Nordihydroguaiaretic acid (NDGA) is a phenolic lignan and possesses antioxidant and number of properties potentially useful to man. The effect of NDGA was studied against norgestrel induced genotoxic damage, using sister chromatid exchanges (SCEs), chromosomal aberrations (CAs), mitotic index (MI) and replication index (RI) as parameters. Amounts of 5, 10 and 20 microM of norgestrel was tested for its genotoxic effect in the absence as well as presence of S9 mix, and was found to be genotoxic at 10 and 20 microM in the presence of S9 mix. Again, 10 microM of norgestrel was treated with 0.5 and 1 microM of NDGA, separately, in the presence of S9 mix. Similar treatment was given with 20 microM of norgestrel. Treatments given with NDGA result in the reduction of SCE, CA and increase of MI as well as RI, suggesting its protective action on human lymphocytes in vitro against the norgestrel induced genotoxic damage.
authors.affiliation: Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202002, Uttar Pradesh, India. yasir_hasansiddique@rediffmail.com
authors.forename: Yasir Hasan
authors.initials: YH
authors.lastname: Siddique
authors.forename: Tanveer
authors.initials: T
authors.lastname: Beg
authors.forename: Mohammad
authors.initials: M
authors.lastname: Afzal
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002453:Cell Cycle; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced; D003280:Contraceptives, Oral, Synthetic / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009637:Masoprocol / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009644:Norgestrel / Q000633:toxicity*; D012854:Sister Chromatid Exchange
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D003280:Contraceptives, Oral, Synthetic; D009644:Norgestrel; D009637:Masoprocol