title: Genomic sequence analysis and organization of BmKalphaTx11 and BmKalphaTx15 from Buthus martensii Karsch: molecular evolution of alpha-toxin genes.
abstract: Based on the reported cDNA sequences of BmKalphaTxs , the genes encoding toxin BmKalphaTx11 and BmKalphaTx15 were amplified by PCR from the Chinese scorpion Buthus martensii Karsch genomic DNA employing synthetic oligonucleotides. Sequences analysis of nucleotide showed that an intron about 500 bp length interrupts signal peptide coding regions of BmKalphaTx11 and BmKalphaTx15. Using cDNA sequence of BmKalphaTx11 as probe, southern hybridization of BmK genome total DNA was performed. The result indicates that BmKalphaTx11 is multicopy genes or belongs to multiple gene family with high homology genes. The similarity of BmKalpha-toxin gene sequences and southern hybridization revealed the evolution trace of BmKalpha-toxins: BmKalpha-toxin genes evolve from a common progenitor, and the genes diversity is associated with a process of locus duplication and gene divergence.
authors.affiliation: Department of Biotechnology, College of Life Sciences, Wuhan University,Wuhan 430072, Hubei Province, PR China.
authors.forename: Xiuling
authors.initials: X
authors.lastname: Xu
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Cao
authors.forename: Jiqun
authors.initials: J
authors.lastname: Sheng
authors.forename: Wenlan
authors.initials: W
authors.lastname: Wu
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Sha
authors.forename: Xin
authors.initials: X
authors.lastname: Mao
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Dahe
authors.initials: D
authors.lastname: Jiang
authors.forename: Wenxin
authors.initials: W
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D017353:Gene Deletion; D020440:Gene Duplication; D016678:Genome; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D012604:Scorpion Venoms / Q000235:genetics*; D012605:Scorpions / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: C505415:BmKalphaTx11 protein, Buthus martensii; C505416:BmKalphaTx15 protein, Buthus martensii; D018076:DNA, Complementary; D012604:Scorpion Venoms