Differential chemical diagnosis of primary hyperoxaluria type II. Highly sensitive analysis of optical isomers of glyceric acid by GC/MS as diastereoisomeric derivatives.
We established a separation method for the optical isomers of glyceric acid in urine by modifying the derivatization steps of the procedure used for the screening and diagnosis. The trimethylsilyl derivatization step in the mass screening procedure was replaced by O-acetyl-(+)-2-butylation, and the samples were analyzed under equivalent GC/MS conditions by capillary gas chromatography on a DB-5MS column. This method can be applied to cases that show a high urinary concentration of glyceric acid to obtain a differential diagnosis of primary hyperoxaluria type II and d-glyceric aciduria easily. l-Glyceric acid was also isolated from the urine of healthy controls as one of the main peaks.
Inoue
Yoshito
Y
Division of Human Genetics, Medical Research Institute, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Ishikawa 920-0293, Japan. yosh@kanazawa-med.ac.jp
Shinka
Toshihiro
T
Ohse
Morimasa
M
Kuhara
Tomiko
T
D003937:Diagnosis, Differential; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005988:Glyceric Acids / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D006960:Hyperoxaluria, Primary / Q000175:diagnosis / Q000652:urine*; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D005988:Glyceric Acids; C042971:glyceric acid