Production of inbred and hybrid transgenic mice carrying large (> 200 kb) foreign DNA fragments by intracytoplasmic sperm injection.
We have developed a mouse transgenesis technique that facilitates the insertion of large (approximately 200 kilo base pairs) DNA fragments into host genomes of both inbred and hybrid mice. Six inbred and three hybrid transgenic mice carrying a single bacterial artificial chromosome (BAC) clone with genes located in the Down syndrome critical region of human chromosome 21 were produced using this technology.
Osada
Tomoharu
T
Department of Anatomy and Reproductive Biology, The Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii, USA.
Toyoda
Atsushi
A
Moisyadi
Stefan
S
Akutsu
Hidenori
H
Hattori
Masahira
M
Sakaki
Yoshiyuki
Y
Yanagimachi
Ryuzo
R
D000818:Animals; D015139:Blotting, Southern; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D017931:DNA Primers; D004229:Dithiothreitol; D004314:Down Syndrome / Q000235:genetics*; D004624:Embryo Transfer; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*
D017931:DNA Primers; D004229:Dithiothreitol