3D-extravasation model -- selection of highly motile and metastatic cancer cells.
Extravasation has been described as a rate-limiting step in the process of hematogeneous metastasis formation. Thereby, transendothelial migration of tumor cells consists of a complex series of events involving multiple cell-cell and cell-matrix interactions. 3D-extravasation assays are valuable tools for the identification of genes, which are the key players at switchboards of the intracellular signaling pathways. In consequence, the combination of 3D-modeling and whole genome expression analysis lead to unravel molecular parameters which descripe distinct clinical phenotypes of cancer and therefore, work as prognosticators, predictors of therapy and new therapy targets.
Brandt
Burkhard
B
Institute of Tumor Biology, Center of Experimental Medicine, University Medical Center, Hamburg-Eppendorf, Martinistr. 52, 20146 Hamburg, Germany.
Heyder
Christoph
C
Gloria-Maercker
Eva
E
Hatzmann
Wolfgang
W
Rötger
Antje
A
Kemming
Dirk
D
Zänker
Kurt S
KS
Entschladen
Frank
F
Dittmar
Thomas
T
D002465:Cell Movement; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D006801:Humans; D021621:Imaging, Three-Dimensional; D018613:Microscopy, Confocal; D008953:Models, Anatomic; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D010766:Phosphorylation; D015398:Signal Transduction; D013997:Time Factors