title: Autoanalyzer for continuous fractionation and quantitation of the polyphenols content in wines.
abstract: A simple continuous flow autoanalyzer for the on-line fractionation of the polyphenols content in wines is proposed. The target compounds are isolated from the matrix by solid-phase extraction on an RP-C18 sorbent column, using selective solvents for the sequential elution of each polyphenol family. Moreover, evaporative light scattering detection (ELSD) is used for the first time for the on-line monitorization of the three polyphenol fractions present in the wine samples. Thus, a single sample injection is required to determine the global concentration of the three selected polyphenol fractions and the whole analysis is completed within a few minutes. Three calibration graphs were constructed for quantitative analysis of the global compounds concentration in every fraction and covered the range 5-300 mg l(-1) (expressed as gallic acid). Average repeatability, expressed as relative standard deviation, was 4%. The proposed autonalyzer was applied to the analysis of a variety of commercial wine samples. The results obtained were compared with those provided by the Folin-Ciocalteau method, being similar in all instances.
authors.affiliation: Department of Analytical Chemistry, Marie Curie Building (Annex), Campus de Rabanales, University of Córdoba, E-14071 Córdoba, Spain. qa1meobj@uco.es
authors.forename: Rafael
authors.initials: R
authors.lastname: Lucena
authors.forename: Soledad
authors.initials: S
authors.lastname: Cárdenas
authors.forename: Mercedes
authors.initials: M
authors.lastname: Gallego
authors.forename: Miguel
authors.initials: M
authors.lastname: Valcárcel
mesh_terms: D000872:Anthocyanins / Q000032:analysis / Q000302:isolation & purification; D001322:Autoanalysis / Q000295:instrumentation*; D005591:Chemical Fractionation / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification*; D044948:Flavonols / Q000032:analysis / Q000302:isolation & purification; D009862:Online Systems / Q000295:instrumentation; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification*; D059808:Polyphenols; D044945:Proanthocyanidins / Q000032:analysis / Q000302:isolation & purification; D027843:Vitis / Q000737:chemistry; D014920:Wine / Q000032:analysis*
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D044948:Flavonols; D010636:Phenols; D059808:Polyphenols; D044945:Proanthocyanidins