title: Adiponectin inhibits the binding of low-density lipoprotein to biglycan, a vascular proteoglycan.
abstract: The aim of this study was to test the possibility that adiponectin has an antiatherogenic effect through the inhibition of LDL binding to proteoglycans, an initial event in atherogenesis. Both full-length and globular adiponectin inhibited LDL binding in a dose-dependent manner. Both types of adiponectin bound to biglycan in a dose-dependent manner. Immunoprecipitation and immunoblotting analysis showed interaction of full-length adiponectin with LDL. Pretreatment of biglycan with globular adiponectin prior to LDL addition diminished the inhibitory effect, while pretreatment with full-length adiponectin retained the effect. This is a new antiatherogenic property that appears independent of the receptor-mediated hormonal action of adiponectin.
authors.affiliation: Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Japan. nihisak@intmed3.med.kyushu-u.ac.jp
authors.forename: Kunihisa
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Toyoshi
authors.initials: T
authors.lastname: Inoguchi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sonoda
authors.forename: Naotaka
authors.initials: N
authors.lastname: Sekiguchi
authors.forename: Hajime
authors.initials: H
authors.lastname: Nawata
mesh_terms: D052242:Adiponectin; D058578:Biglycan; D004305:Dose-Response Relationship, Drug; D024202:Electrophoretic Mobility Shift Assay; D016326:Extracellular Matrix Proteins; D047468:Immunoprecipitation; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D011509:Proteoglycans / Q000378:metabolism*
chemical_list: D052242:Adiponectin; D058578:Biglycan; D016326:Extracellular Matrix Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008077:Lipoproteins, LDL; D011509:Proteoglycans