Long-term survival of bone marrow-derived retinal nerve cells in the retina.
Recently, we have demonstrated that bone marrow stem cells can differentiate into retinal nerve cells. In the present study, we show a new and efficient strategy for transplanting bone marrow stem cells into the retina. When bone marrow stem cells were injected into the vitreous cavity of untreated eyes, only very few cells were found in the retina 2 weeks after injection. In contrast, when laser photocoagulation was performed just before the injection of bone marrow stem cells, a large number of the injected cells survived 2 weeks after injection and the cells expressed neural cell-specific or retinal nerve cell-specific antigens. Moreover, we still detected bone marrow stem cell-derived retinal nerve cells in the retina 1 year after injection in the retina.
Minamino
Keizo
K
First Department of Pathology, Kansai Medical University, Moriguchi city, Osaka, Japan.
Adachi
Yasushi
Y
Yamada
Haruhiko
H
Higuchi
Akiko
A
Suzuki
Yasuhiro
Y
Iwasaki
Masayoshi
M
Nakano
Keiji
K
Koike
Yasushi
Y
Mukaide
Hiromi
H
Kiriyama
Naoko
N
Shigematsu
Akio
A
Matsumura
Miyo
M
Ikehara
Susumu
S
D000704:Analysis of Variance; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D002452:Cell Count / Q000379:methods; D006085:Graft Survival / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008028:Light Coagulation / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000502:physiology*; D012160:Retina / Q000166:cytology*; D012243:Rhodopsin / Q000378:metabolism; D013234:Stem Cells / Q000502:physiology*; D013997:Time Factors
D007381:Intermediate Filament Proteins; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D049452:Green Fluorescent Proteins; D012243:Rhodopsin