Aptameric GT oligomers need to be complexed to ethoxylated polyethylenimine as pre-paired duplexes to efficiently exert their cytotoxic activity in human lymphoblastic cancer cells.
The aptameric oligonucleotides GT were found to exert a selective, specific and dose-dependent cell growth inhibition effect on a variety of human cancer cells by recognising specific nuclear proteins and among these in particular an isoform of the eukaryotic elongation factor 1A1 (EEF1A1). The potential development of these aptameric oligomers needs that they retain serum and intracellular stabilities. Polycations are safe non-viral carriers of the nucleic acids. We demonstrated that a weakly basic polycation, the ethoxylated polyethylenimine (EPEI), can efficiently deliver cytotoxic GT oligomers when they were complexed as partial pre-paired duplex. In this way, nuclease-resistance of the oligomer was markedly improved and the administration of the duplex complexed with EPEI to lymphoblastic cancer cells caused a specific cytotoxic effect at concentrations lower than that of naked GT. However, the cytotoxic activity of the oligomer-EPEI complex resulted strictly related to the GC content and Tm of the duplex region. The single-stranded GT and the duplex with high GC content and Tm, although complexed with EPEI failed to exert cytotoxicity. Overall results indicated that aptameric oligomers complexed with polycations can be efficiently delivered into the cells and display the desired biological effect designing a balanced partial duplex whose stability can allow oligomer release from the polycation under the physiological cellular conditions.
Scaggiante
Bruna
B
Molecular Biology Section, Department of Biochemistry, Biophysics and Macromolecular Chemistry, via Giorgeri, 1, University of Trieste, 34127 Trieste, Italy.
Dapas
Barbara
B
Perissin
Laura
L
Manzini
Giorgio
G
D001483:Base Sequence; D016923:Cell Death; D002457:Cell Extracts; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006147:Guanine / Q000378:metabolism; D006207:Half-Life; D006801:Humans; D015458:Leukemia, T-Cell / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry* / Q000494:pharmacology; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine / Q000737:chemistry* / Q000494:pharmacology; D013941:Thymine / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D002457:Cell Extracts; D006147:Guanine; D009687:Nuclear Proteins; D009841:Oligonucleotides; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine; D013941:Thymine