Staphylococcal enterotoxin A modulates intracellular Ca2+ signal pathway in human intestinal epithelial cells.
We demonstrate here that staphylococcal enterotoxin A (SEA) induces an increase in intracellular calcium ([Ca2+]i) in human intestinal epithelial cells and the [Ca2+]i is released from intracellular stores. SEA-induced increase of [Ca2+]i was clearly inhibited by treatment with a nitric oxide synthase (NOS) inhibitors, N(G)-monomethyl-L-arginine and guanidine. Intestinal epithelial cells express endothelial NOS in resting cell condition, and express inducible NOS after stimulating with tumor necrosis factor (TNF)-alpha. TNF-alpha-pretreated cells showed a significant increase in [Ca2+]i that was also inhibited by the NOS inhibitor. These results suggest that SEA modulated [Ca2+]i signal is dependent on NOS expression in human intestinal epithelial cells.
Hu
Dong-Liang
DL
Department of Bacteriology, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan.
Suga
Sechiko
S
Omoe
Katsuhiko
K
Abe
Yoshinao
Y
Shinagawa
Kunihiro
K
Wakui
Makoto
M
Nakane
Akio
A
D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects*; D002413:Cations, Divalent / Q000378:metabolism; D004768:Enterotoxins / Q000494:pharmacology*; D019791:Guanidine / Q000494:pharmacology; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D017136:Ion Transport; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D019323:omega-N-Methylarginine / Q000494:pharmacology
D002413:Cations, Divalent; D004768:Enterotoxins; D014409:Tumor Necrosis Factor-alpha; D019323:omega-N-Methylarginine; C026066:enterotoxin A, Staphylococcal; D019001:Nitric Oxide Synthase; D019791:Guanidine; D002118:Calcium