[Nuclear localization region in soluble adenylyl cyclase].
OBJECTIVE
To locate the region responsible for nuclear localization of protein sAC.


METHODS
The eukaryotic expression vector of vairous sAC deletion mutants were transfected into Hela cells. The localization of each mutant was observed using confocal microscope.


RESULTS
For some mutants, the localization of sAC changed. Deletion of some region made it unable to locate in the nuclear.


CONCLUSIONS
It is possible to figure out that the nucleotide region (739-1038 and 1045-1261) take charge of nuclear localization of sAC.
Feng
Qi-ping
QP
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Zuo
Jin
J
Meng
Yan
Y
Fang
Fu-de
FD
D000262:Adenylyl Cyclases / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005820:Genetic Testing; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D009687:Nuclear Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D009687:Nuclear Proteins; D000262:Adenylyl Cyclases