Determination of puerarin in rat cortex by high-performance liquid chromatography after intravenous administration of Puerariae flavonoids.
In the present study, a rapid and simple high-performance liquid chromatographic (HPLC) assay for determination of puerarin in rat cortex was developed. The analysis was carried out on a Zorbax SB-C18 column with mobile phase acetonitrile-0.5% aqueous phosphoric acid (11:89, v/v). The detection was by UV at 252 nm. The calibration curve for puerarin was linear (r=0.9999) over the concentration range 0.516-206.250 microg/mL. The limit of detection was 0.206 microg/mL (signal-to-noise ratio 3) and the limit of quantification (signal-to-noise ratio 10) was 0.516 microg/mL. Stability studies showed that puerarin was stable at temperatures of 4 degrees C in methanol for at least 30 days. The intra- and inter-day assays of puerarin from rat cortex were less than 2.5% at concentration range 0.516-206.250 microg/mL and good overall recoveries (97.4-101.7%) were found at same concentrations. The method was applied to determine the pharmacokinetic parameters and the time course of puerarin in rat cortex, following a single dosage of intravenous administration of flavonoids from Puerariae radix at 32 mg/kg of puerarin to male Wistar rats.
Yan
Bin
B
Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
Wang
Wei
W
Zhang
Lujun
L
Xing
Dongming
D
Wang
Daoyi
D
Du
Lijun
L
D000818:Animals; D002540:Cerebral Cortex / Q000737:chemistry*; D005419:Flavonoids / Q000008:administration & dosage*; D007275:Injections, Intravenous; D007529:Isoflavones / Q000032:analysis* / Q000493:pharmacokinetics; D008297:Male; D029908:Pueraria / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
D005419:Flavonoids; D007529:Isoflavones; C033607:puerarin