Are microproerythroblasts in human bone marrow real or artefacts? A cytochemical note.
Early erythroid precursors were studied in human bone marrow smears to provide more information on small proerythroblasts--"microproerythroblasts"--using a silver reaction to demonstrate silver stained nucleolar organizer regions (AgNORs) and light microscopic densitometry of large irregularly shaped nucleoli and cytoplasm stained for RNA. No significant differences were found for the density of such nucleoli and basophilic cytoplasm between characteristic large proerythroblasts with a nuclear diameter larger that 9 microm (K2 and K1 erythroblasts) and small proerythroblasts--"microproerythroblasts" representing a subpopulation of K1/2 erythroblasts (early basophilic erythroblasts), which are characterized by a smaller nuclear diameter. In addition, large irregularly shaped nucleoli of "microproerythroblasts" possessed numerous silver stained particles representing AgNORs similar to those of large proerythroblasts. The number of AgNORs in "microproerythroblasts" was slightly, but significantly, smaller than that in large characteristic proerythroblasts.
Smetana
Karel
K
Clinical Department, Institute of Haematology and Blood Transfusion, U nemocnice 1, Prague 2, 128 20 Czech Republic. karel.smetana@ukht.cz
Jirásková
Ilona
I
Klamová
Hana
H
D016477:Artifacts; D001854:Bone Marrow Cells / Q000166:cytology* / Q000473:pathology / Q000648:ultrastructure; D002460:Cell Line; D002466:Cell Nucleolus / Q000737:chemistry / Q000648:ultrastructure; D003593:Cytoplasm / Q000737:chemistry / Q000648:ultrastructure; D004900:Erythroblasts / Q000166:cytology* / Q000473:pathology / Q000648:ultrastructure; D006651:Histocytochemistry; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D018613:Microscopy, Confocal; D012313:RNA / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods
D012834:Silver; D012313:RNA