Identification of RNA-binding regions on the P and V proteins of human parainfluenza virus type 2.
We have shown that the P and V proteins of human parainfluenza virus type 2 (hPIV-2) bind to genomic RNA by using Northwestern blot analysis. To identify the RNA-binding regions on the P and V proteins, we used a set of deletion mutants produced in Escherichia coli. One region required for the RNA-binding was found in the P-V common domain (aa 1-82). Others were found in the P protein-specific region (aa 249-354) and the V protein-specific region (aa 176-225). In addition, we have shown that substitutions of some basic residues with alanines in these regions abrogate RNA-binding by the P or V proteins. Intriguingly, the P and V proteins of hPIV2 can selectively bind to the viral RNA under our experimental conditions.
Nishio
Machiko
M
Department of Microbiology, Mie University School of Medicine, 2-174, Edobashi, Tsu, Mie 514-8507, Japan. nishio@doc.medic.mie-u.ac.jp
Tsurudome
Masato
M
Ito
Morihiro
M
Ito
Yasuhiko
Y
D019943:Amino Acid Substitution; D015152:Blotting, Northern; D015153:Blotting, Western; D016297:Mutagenesis, Site-Directed; D010223:Parainfluenza Virus 2, Human / Q000502:physiology*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
C407030:P protein, Human parainfluenza virus 2; D010750:Phosphoproteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; C068854:V protein, human parainfluenza virus type 1; D014764:Viral Proteins