The lipolytic proteome of mouse adipose tissue.
Hydrolysis of triacylglycerols and cholesteryl esters is a key event in energy homeostasis of animals. However, many lipolytic activities still await their molecular identification. Here we report on a novel tool for concomitant analysis of lipases in complex proteomes. Fluorescent activity tags mimicking lipid substrates were used to label the proteome of mouse adipose tissue. Analysis by two-dimensional gel electrophoresis and LC-MS/MS led to the identification of all known intracellular lipases as well as a number of novel candidates. One of them was recently shown to be involved in triacylglycerol mobilization in adipocytes and therefore named adipose triglyceride lipase. Functional characterization of expressed enzymes demonstrated that lipolytic and esterolytic activities could be well discriminated. Thus our results show the first map of the lipolytic proteome of mouse adipose tissue and demonstrate the general applicability of our method for rapid profiling and identification of lipolytic activities in complex biological samples.
Birner-Gruenberger
Ruth
R
Institute of Biochemistry, Graz University of Technology, Petersgasse 12/2, Graz, Austria.
Susani-Etzerodt
Heidrun
H
Waldhuber
Markus
M
Riesenhuber
Gernot
G
Schmidinger
Hannes
H
Rechberger
Gerald
G
Kollroser
Manfred
M
Strauss
Juliane G
JG
Lass
Achim
A
Zimmermann
Robert
R
Haemmerle
Guenter
G
Zechner
Rudolf
R
Hermetter
Albin
A
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D008049:Lipase / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects; D051379:Mice; D018345:Mice, Knockout; D020543:Proteome / Q000378:metabolism*; D002787:Sterol Esterase / Q000172:deficiency / Q000378:metabolism
D020543:Proteome; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; D002787:Sterol Esterase; D008049:Lipase; D009327:4-Chloro-7-nitrobenzofurazan