Host humoral immune response to Leishmania lipid-binding protein.
SUMMARY We report on the use of Leishmania donovani lipid-binding proteins (LBPs) as antigens capable of being recognized by serum from immunocompetent patients from southern Spain suffering from visceral leishmaniasis and from Peruvian patients with localized cutaneous leishmaniasis caused by Leishmania braziliensis. The absorbance found by immunoenzymatic techniques gave significantly different results for the serum samples from patients with and without leishmaniasis. Specificity by ELISA testing was 93.2% and sensibility 100%. Dot blots from human patient serum samples or naturally infected dogs from Spain gave similarly significant results. All the human serum samples from individuals with visceral leishmaniasis and the Leishmania-positive canine samples recognized two bands, with molecular weights of 8 and 57 kDa. The serum from individuals with cutaneous leishmaniasis caused by L. braziliensis recognized an additional band of 16 kDa. We discuss the role of Leishmania FABP and compare the immunological reactions found with serum samples from other protozoan infections such as toxoplasma and Chagas as well as bacterial infections such as tuberculosis and syphilis.
Maache
M
M
Instituto de Biotecnología, Grupo de Bioquímica y Parasitología Molecular, Universidad de Granada, Granada, Spain.
Azzouz
S
S
Diaz de la Guardia
R
R
Alvarez
P
P
Gil
R
R
de Pablos
L M
LM
Osuna
A
A
D000293:Adolescent; D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001499:Bayes Theorem; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D050556:Fatty Acid-Binding Proteins; D006801:Humans; D007892:Leishmania braziliensis / Q000276:immunology*; D007893:Leishmania donovani / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000276:immunology*; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins