Functional analysis of pathogenicity proteins of the potato cyst nematode Globodera rostochiensis using RNAi.
RNA interference (RNAi) has been used widely as a tool for examining gene function and a method that allows its use with plant-parasitic nematodes recently has been described. Here, we use a modified method to analyze the function of secreted beta-1,4, endoglucanases of the potato cyst nematode Globodera rostochiensis, the first in vivo functional analysis of a pathogenicity protein of a plant-parasitic nematode. Knockout of the beta-1,4, endoglucanases reduced the ability of the nematodes to invade roots. We also use RNAi to show that gr-ams-1, a secreted protein of the main sense organs (the amphids), is essential for host location.
Chen
Qing
Q
Plant-Pathogen Interactions Programme, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK.
Rehman
S
S
Smant
G
G
Jones
John T
JT
D000818:Animals; D001483:Base Sequence; D002480:Cellulase / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics*; D010935:Plant Diseases / Q000469:parasitology; D034622:RNA Interference; D011198:Solanum tuberosum / Q000469:parasitology*; D014431:Tylenchoidea / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence
D018509:DNA, Helminth; D015801:Helminth Proteins; D002480:Cellulase