title: GAL4-GFP enhancer trap lines for genetic manipulation of lateral root development in Arabidopsis thaliana.
abstract: Lateral root development occurs throughout the life of the plant and is responsible for the plasticity of the root system. In Arabidopsis thaliana, lateral root founder cells originate from pericycle cells adjacent to xylem poles. In order to study the mechanisms of lateral root development, a population of Arabidopsis GAL4-GFP enhancer trap lines were screened and two lines were isolated with GAL4 expression in root xylem-pole pericycle cells (J0121), i.e. in cells competent to become lateral root founder cells, and in young lateral root primordia (J0192). These two enhancer trap lines are very useful tools with which to study the molecular and cellular bases of lateral root development using targeted gene expression. These lines were used for genetic ablation experiments by targeting the expression of a toxin-encoding gene. Moreover, the molecular bases of the enhancer trap expression pattern were characterized. These results suggest that the lateral-root-specific GAL4 expression pattern in J0192 is due to a strong enhancer in the promoter of the LOB-domain protein gene LBD16.
authors.affiliation: UMR 1098, Institut de Recherche pour le Développement, 911 Avenue Agropolis, F-34394 Montpellier cedex 5, France. laplaze@mpl.ird.fr
authors.forename: Laurent
authors.initials: L
authors.lastname: Laplaze
authors.forename: Boris
authors.initials: B
authors.lastname: Parizot
authors.forename: Andrew
authors.initials: A
authors.lastname: Baker
authors.forename: Lilian
authors.initials: L
authors.lastname: Ricaud
authors.forename: Alexandre
authors.initials: A
authors.lastname: Martinière
authors.forename: Florence
authors.initials: F
authors.lastname: Auguy
authors.forename: Claudine
authors.initials: C
authors.lastname: Franche
authors.forename: Laurent
authors.initials: L
authors.lastname: Nussaume
authors.forename: Didier
authors.initials: D
authors.lastname: Bogusz
authors.forename: Jim
authors.initials: J
authors.lastname: Haseloff
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins; D004742:Enhancer Elements, Genetic; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D016254:Mutagenesis, Insertional; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins