[Repair of bone defect with allograft demineralized bone containing basic fibroblast growth factor in rabbits].
OBJECTIVE
To evaluate the ability of inductive osteogenesis of allograft demineralized bone containing basic fibroblast growth factor (bFGF/ALB) in repairing bone defect.


METHODS
Thirty-two New Zealand white rabbits were randomly divided into four groups (groups A,B,C and D, n=8). A segmental bone defect of 15 mm in length was made on the bilateral radius respectively and the defects filled with ALB/bFGF in group A, with ALB in group B, with bFGF in group C and without any materials in group D serving as blank control. At 2, 4, 6 and 8 weeks after operation, all restored bones were evaluated by roentgenography, histological observation and Ca2+ detection of osteotylus.


RESULTS
The X-ray films showed that groups A and B had a little shadow of bone formation at 2 weeks, while groups C and D had transparent shadow; that group A had denser shadow and new bone formation at 4 weeks and 6 weeks, groups B and C had a little increase of shadow and group D had little shadow at fractured ends; and that group A had formation of bone bridge at 8 weeks, the new formed bone in fractured ends of group B closed with each other, the gap still existed in group C, and the defects filled with the soft tissue in group D. The Ca2+ content of group A was higher than that of groups B, C and D at 4 weeks (P<0.05) and 8 weeks (P<0.01). The histological observation showed that the degree of bone restoration of group A was superior to that of groups B, C and D.


CONCLUSIONS
bFGF/ALB is a good material to improve bone restoration.
Li
Feng
F
Department of Material, College of Stomatology, Fourth Military Medical University, Xi'an Shaanxi, 710032, P. R. China.
Zhao
Xinyi
X
Wu
Junzheng
J
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials; D018775:Bone Demineralization Technique; D001861:Bone Regeneration; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000295:instrumentation / Q000379:methods*; D004195:Disease Models, Animal; D016222:Fibroblast Growth Factor 2; D048091:Guided Tissue Regeneration; D011817:Rabbits; D011884:Radius / Q000293:injuries / Q000503:physiopathology / Q000601:surgery*; D011897:Random Allocation; D023822:Tissue Engineering; D014184:Transplantation, Homologous
D001672:Biocompatible Materials; D018786:Bone Substitutes; D016222:Fibroblast Growth Factor 2