Preparation of de novo globular proteins based on proline dendrimers.
A synthetic method for the preparation of protein-like globular dendrimers derived from a combination of proline, glycine and imidazolidin ring as branching unit is described. The methodology allows the synthesis of novel peptide dendrimers up to fourth generation. Dendrimers were synthesized by a convergent solid-phase peptide synthesis approach. The conformational properties of branched polyproline peptides and proline dendrimers were studied by CD experiments. CD data suggest conformational plasticity of branched peptides for PPI and PPII, and a stable well-defined secondary structure of proline dendrimers for PPII.
Sanclimens
Gloria
G
Barcelona Biomedical Research Institute, Barcelona Science Park, University of Barcelona, Josep Samitier 1-5, 08028 Barcelona, Spain.
Crespo
Laia
L
Giralt
Ernest
E
Albericio
Fernando
F
Royo
Miriam
M
D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D015394:Molecular Structure; D011392:Proline / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D011392:Proline