Determination of co-receptor usage of HIV-1.
In addition to CD4, HIV-1 uses chemokine receptors for entry in their target cells. The most important chemokine receptors in this respect are beta-chemokine receptor 5 (CCR5) and alpha-chemokine receptor 4 (CXCR4). Coreceptor usage is an important feature of the biological phenotype of HIV-1 variants. In this chapter, methods are described to determine the co-receptor usage of HIV-1 variants.
Schuitemaker
Hanneke
H
Department of Clinical Viro-Immunology Sanquin Research at CLB, Academical Medical Center, Amsterdam, The Netherlands.
Kootstra
Neeltje A
NA
D065100:CCR5 Receptor Antagonists; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D005838:Genotype; D015497:HIV-1 / Q000378:metabolism*; D006720:Homozygote; D006801:Humans; D019713:Receptors, CCR5 / Q000235:genetics* / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D065100:CCR5 Receptor Antagonists; D019713:Receptors, CCR5; D019718:Receptors, CXCR4