title: Plasma concentrations of vascular endothelial growth factor and basic fibroblast growth factor in lymphoproliferative disorders.
abstract: Angiogenesis plays a major role in the development and progression of haematological malignancies. In our study we measured plasma concentrations of key angiogenic activators vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) using comercially available sandwich enzyme-linked immunosorbent assay (ELISA) in 37 patients with lymphoid malignancies and 20 healthy donors. We found a statistically significant increase in bFGF concentrations in patients with B-cell chronic lymphocytic leukemia (B-CLL, n=18) compared to the control group (median 118.8 vs. 9.3 pg/ml, p<0.001). However, we didn't find any significant difference in VEGF concentrations between B-CLL patients and the control group. There was also no significant increase in bFGF or VEGF in patients with multiple myeloma (n=7) and non-Hodgkin's lymphoma (n=12). Our pilot study shows that measurement of angiogenic activators in plasma is a feasible and reproducible method of angiogenesis assessment. Larger studies are needed for correlation between serum and plasma concentrations and detailed statistical evaluation including the impact on patients' survival.
authors.affiliation: Charles University in Prague, Faculty of Medicine in Hradec Králové, 2nd Department of Internal Medicine, Hradec Králové, Czech Republic. smolej@seznam.cz
authors.forename: Lukás
authors.initials: L
authors.lastname: Smolej
authors.forename: Ctirad
authors.initials: C
authors.lastname: Andrýs
authors.forename: Vladimír
authors.initials: V
authors.lastname: Maisnar
authors.forename: Ludĕk
authors.initials: L
authors.lastname: Pour
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Malý
mesh_terms: D016222:Fibroblast Growth Factor 2 / Q000097:blood*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood; D008228:Lymphoma, Non-Hodgkin / Q000097:blood; D008232:Lymphoproliferative Disorders / Q000097:blood*; D009101:Multiple Myeloma / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2