[Effect of varicocele on expression of apoptosis associated gene Bcl-2 and Bax in spermatogenic cells of adolescent rats].
OBJECTIVE
To study the effect of varicocel (VC) on expression of apoptosis associated gene Bcl-2 and Bax in spermatogenic cells of adolescent rats, and investigate the mechanism of the infertility resulting from varicocele.


METHODS
The varicocele model was created by partial ligation of the left renal vein in the adolescent rats, two and four and eight weeks after creation of the varicocele model, the expressions of Bcl-2 and Bax were detected by immunohistochemical method.


RESULTS
With the duration of varicocele model, the Bcl-2 expression in experiment group was decreased as compared with that in control group. And in experiment group, the Bcl-2 expression level was lower in left testis than in right testis. But Bax expression in experiment group was increased as compared with that in control group, and in experiment group, the Bax expression level was higher in left testis than in right testis.


CONCLUSIONS
Experimental varicocele could change obviously expression of apoptosis associated gene Bcl-2 and Bax in spermatogenic cells of adolescent rats.
He
Xue-jun
XJ
Department of Urology, The First Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China.
Chen
Le-zhong
LZ
Fang
De-xin
DX
Ye
He-song
HS
She
Shao-yi
SY
Hong
Han-ye
HY
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013091:Spermatogenesis; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology*; D014646:Varicocele / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis* / Q000235:genetics
D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein