[Creation and hybrid identification of the sexual progenies using citrus somatic hybrid as pollen parent].
110 plantlets were obtained from the cross between citrus allotetraploid somatic hybrid [Hamlin sweet orange (Citrus sinensis Osback) + Rough Lemon (C. jambhiri Luss)] (as the pollen parent) and mono-embryonic diploid type Iben No.4 [Huanongbendizao tangerine (C. reticulata Blanco) x Ichang papeda (C. ichangensis Swingle)] by the means of young embryo culture in vitro, and there were 93 triploids and 17 diploids through chromosome counting and the ploidy analyser identification. RAPD analyses showed that all sexual progenies were hybrid.
Yi
Hua Lin
HL
National Key Laboratory on Crop Genetic Improvement, National Citrus Breeding Center, Huazhong Agricultural University, Wuhan 430070. yihualin@mail.hzau.edu.cn
Deng
Xiu Xin
XX
D032461:Chromosomes, Plant / Q000235:genetics; D002957:Citrus / Q000235:genetics*; D004171:Diploidy; D006824:Hybridization, Genetic / Q000235:genetics*; D011058:Pollen / Q000235:genetics / Q000502:physiology*; D011123:Polyploidy; D019105:Random Amplified Polymorphic DNA Technique