Determination of norfloxacin using a terbium-sensitized electrogenerated chemiluminescence method.
A simple electrogenerated chemiluminescence (ECL) analysis method for the determination of norfloxacin (NFLX) is reported. It is based on ECL produced by Na(2)SO(3), which is sensitized by the Tb-NFLX complex. The relative ECL intensity of the Tb(3+)-NFLX-Na(2)SO(3) system is proportional to the amount of NFLX. The optimized experimental conditions were investigated. The linear range and detection limit for NFLX were 1.0 x 10(-10)-8.0 x 10(-7) mol/L and 2.8 x 10(-11) mol/L, respectively. This method was successfully applied to the determination of NFLX in a capsule. NFLX in urine can be directly detected without pretreatment or separation.
Chen
Shi-Lv
SL
Department of Chemistry, Beijing Normal University, Beijing 100875, People's Republic of China.
Liu
Yu
Y
Zhao
Hui-Chun
HC
Jin
Lin-Pei
LP
Zhang
Zhong-Lun
ZL
Zheng
Yan-Zhen
YZ
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D009643:Norfloxacin / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry; D013725:Terbium / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D013431:Sulfates; D013725:Terbium; C012036:sodium sulfate; D009643:Norfloxacin