title: Downregulation of the major histocompatibility complex class I molecules by human herpesvirus type 8 and impaired natural killer cell activity in primary effusion lymphoma development.
abstract: Primary effusion lymphomas (PELs) are invariably infected by human herpesvirus type 8 (HHV8) and often co-infected by Epstein-Barr virus (EBV). We found that expression of major histocompatibility complex class I (MHC-I) surface molecules was significantly decreased in PEL cells when compared with HHV8 negative lymphomas, irrespective of EBV infection. MHC-I downregulation rendered PEL cells sensitive to recognition and killing by natural killer (NK) cells. Intriguingly, analysis of MHC-I non-restricted cytotoxicity in two PEL patients indicated a reduced NK cell activity when compared with healthy individuals. These data suggest that PEL outgrowth may require an impaired NK cell function.
authors.affiliation: Clinical Immunology, Department of Clinical Medicine, University of Roma La Sapienza, Italy. mariacaterina.sirianni@uniroma1.it
authors.forename: Maria Caterina
authors.initials: MC
authors.lastname: Sirianni
authors.forename: Fabio
authors.initials: F
authors.lastname: Libi
authors.forename: Massimo
authors.initials: M
authors.lastname: Campagna
authors.forename: Davide
authors.initials: D
authors.lastname: Rossi
authors.forename: Daniela
authors.initials: D
authors.lastname: Capello
authors.forename: Gaia
authors.initials: G
authors.lastname: Sciaranghella
authors.forename: Antonio
authors.initials: A
authors.lastname: Carbone
authors.forename: Cecilia
authors.initials: C
authors.lastname: Simonelli
authors.forename: Paolo
authors.initials: P
authors.lastname: Monini
authors.forename: Gianluca
authors.initials: G
authors.lastname: Gaidano
authors.forename: Barbara
authors.initials: B
authors.lastname: Ensoli
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D003601:Cytotoxicity Tests, Immunologic; D004279:DNA, Viral / Q000032:analysis; D015536:Down-Regulation; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis / Q000276:immunology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000235:genetics; D019288:Herpesvirus 8, Human / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D016393:Lymphoma, B-Cell / Q000276:immunology* / Q000821:virology*; D012698:Serologic Tests
chemical_list: D015415:Biomarkers; D004279:DNA, Viral; D015395:Histocompatibility Antigens Class I