[Therapeutic effect of qingkailing and shengmai injection alone or combined on the acute lung injury induced by oleic acid in rabbits].
OBJECTIVE
To investigate the effect of Qingikailing and Shengmai injection alone or combined on the acute lung injury (AL) induced by oleic acid in rabbits.


METHODS
The rabbits were randomly divided into 11 groups: oleic acid group; control group; treatment groups including low, middle and high dosage groups of Qingkailing and Shengmai injection alone and combined, respectively. ALI model was established by iv oleic acid (0.05 mL x kg(-1)) in these groups, and then iv above drugs respectively,while in control group, the same volume of normal saline was given. The respiratory amplitude and rate were observed, and blood samples were taken from cervical artery for blood-gas analysis before and at 30, 60, 120 min after oleic acid or normal saline administration. At the end of experiment, the concentration of LDH, CAT and MDA in the lung tissue were measured and pathologic changes of lung tissue were observed microscopically.


RESULTS
Compared with oleic acid group, the respiratory amplitude markedly enhanced (P < 0.05) in the low and high dose groups of Qingkailing and Shengmai injection. PaO2 increased significantly (P < 0.05) in the low dose group of combined Qingkailing and Shengmai injection, PaCO2 decreased markedly (P < 0.05) in the low dose groups of Qingkailing and Shengmai injection alone and combined. The level of MDA significantly decreased (P < 0.05) in the each group of Qingkailing and Shengmai injection alone, the level of MDA significantly decreased (P < 0.05) and CAT increased (P < 0.05) in the low dose group of combined Qingkailing with Shengmai injection. The low dose group of combined Qingkailing and Shengmai injection can alleviate the pathological changes induced by oleic acid.


CONCLUSIONS
The curative effect of the low dose group of combined Qingkailing with Shengmai injection for the ALI induced by oleic acid was better than Qingkailing and Shengmai injection alone at the same dosage.
Zhang
Chun-ying
CY
Institute of Chinese Material Medica, China Academy of Traditional Chinese Medicine, Beiing 100700, China.
Du
Gui-you
GY
Lang
Ri-xin
RX
Wang
Xiu-rong
XR
Gao
Wei
W
Cui
Hai-feng
HF
Zhao
Yong
Y
Wu
Zi-lun
ZL
He
Rong
R
Gao
Shuang-rong
SR
Hui
Lian-qiang
LQ
Zhu
Xiao-xin
XX
D000818:Animals; D002245:Carbon Dioxide / Q000097:blood; D002374:Catalase / Q000097:blood; D004338:Drug Combinations; D004359:Drug Therapy, Combination; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D019301:Oleic Acid; D010100:Oxygen / Q000097:blood; D010946:Plants, Medicinal / Q000737:chemistry; D011817:Rabbits; D011897:Random Allocation; D012119:Respiration / Q000187:drug effects*; D012128:Respiratory Distress Syndrome / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D002245:Carbon Dioxide; D019301:Oleic Acid; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D010100:Oxygen