Visualization of infectious pancreatic necrosis virus (IPNV) particles labeled with fluorescent probes.
Infectious pancreatic necrosis virus (IPNV) particles were labeled with SYBR Green I or a monoclonal antibody and FITC-conjugated secondary antibody and examined in a fluorescence microscope. Labeled viral particles were visualized in a narrow range of pixels. Comparing IPNV particles with fluorescent phage T4 virions, the former, as expected, were seen smaller in size. The method allows the rapid and accurate counting of viral particles both on filters and bound to the cell surface. In addition, IPNV particles can be specifically enumerated in the presence of other virions and the ratio between physical particles and virus infectivity can be easily calculated as well.
Osorio
Manuel
M
Laboratorio de Bioquímica y Virología, Instituto de Ciencias Biológicas y Químicas, Facultad de Ciencias, Universidad de Valparaíso, Casilla 50 30, Valparaíso, Chile.
Espinoza
Juan Carlos
JC
Kuznar
Juan
J
D000818:Animals; D052160:Benzothiazoles; D002460:Cell Line; D003959:Diamines; D018061:Infectious pancreatic necrosis virus / Q000302:isolation & purification*; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals; D011804:Quinolines; D013194:Staining and Labeling / Q000379:methods*
D052160:Benzothiazoles; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I