title: Product and product-independent induction of butane oxidation in Pseudomonas butanovora.
abstract: Pseudomonas butanovora grows on butane by means of an inducible soluble alkane monooxygenase (sBMO). The induction of sBMO was studied using the wild type and a sBMO reporter strain. The reporter strain has the lacZ::kan cassette inserted into bmoX, the gene that encodes the alpha-subunit of the hydroxylase of sBMO. The beta-galactosidase activity in the reporter strain was not induced by butane, but was induced by 1-butanol and butyraldehyde. P. butanovora expressed sBMO product-independent activity at 3.0+/-1 nmol ethylene oxide min(-1) mg protein(-1) in stationary phase. The sBMO product-independent activity likely primes the expression of sBMO by butane.
authors.affiliation: Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA. sayavedl@science.oregonstate.edu
authors.forename: Luis A
authors.initials: LA
authors.lastname: Sayavedra-Soto
authors.forename: David M
authors.initials: DM
authors.lastname: Doughty
authors.forename: Elizabeth G
authors.initials: EG
authors.lastname: Kurth
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Bottomley
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Arp
mesh_terms: D020001:1-Butanol / Q000378:metabolism / Q000494:pharmacology; D000447:Aldehydes / Q000378:metabolism / Q000494:pharmacology; D002073:Butanes / Q000378:metabolism* / Q000494:pharmacology; D042926:Cytochrome P-450 CYP4A / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D017930:Genes, Reporter; D007763:Lac Operon; D008954:Models, Biological; D016254:Mutagenesis, Insertional; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012995:Solubility
chemical_list: D000447:Aldehydes; D002073:Butanes; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; C046888:butane; D020001:1-Butanol; D042926:Cytochrome P-450 CYP4A; C018475:butyraldehyde