Bovine viral diarrhea virus entry is dependent on clathrin-mediated endocytosis.
Cellular mechanisms of bovine viral diarrhea virus (BVDV) entry in MDBK cells were investigated. Chloroquine, bafilomycin A1, or ammonium chloride inhibited BVDV infection, indicating that an acidic endosomal pH is required for BVDV entry. The tyrosine kinase inhibitor genistein partially inhibited BVDV infection at a postentry step, whereas BVDV entry was strongly inhibited by chlorpromazine or by the overexpression of a dominant-negative form of EPS15, a protein essential for the formation of clathrin-coated vesicles at the plasma membrane. Together, these data indicate that BVDV infection requires an active clathrin-dependent endocytic pathway.
Lecot
Steve
S
CNRS-UPR2511, Institut de Biologie de Lille, Lille Cedex, France.
Belouzard
Sandrine
S
Dubuisson
Jean
J
Rouillé
Yves
Y
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002746:Chlorpromazine / Q000494:pharmacology; D002966:Clathrin / Q000502:physiology*; D001908:Diarrhea Viruses, Bovine Viral / Q000502:physiology*; D004705:Endocytosis; D019833:Genistein / Q000494:pharmacology
D002966:Clathrin; D019833:Genistein; D002746:Chlorpromazine