title: Antimicrobial effects and human gingival biocompatibility of hydroxyapatite sol-gel coatings.
abstract: The sol-gel method was employed to synthesize hydroxyapatite (HAp) coatings modified with Ag or Zn ions onto Ti-6Al-4V substrate. A bacterial strain Streptococcus mutans (S. mutans) and a human gingival fibroblast (HGF-1) cell line were used to investigate the antimicrobial effect and biocompatibility, respectively. HAp coatings containing 100 ppm Ag(+) ions suppressed the growth of S. mutans. An apparent inhibition zone around the HAp coating was further observed at Ag(+) concentration up to 10,000 ppm. However, for coatings containing Zn(2+) ions, a clear inhibition zone was observed at Zn(2+) concentration of 10,000 ppm. Nevertheless, the results of HGF-1 cultivation demonstrated that the Zn(2+)-modified HAp coatings exhibited better attachment and spread of HGF-1 than did the Ag(+)-modified coatings. Zn(2+) modified HAp coatings also increased the plating efficiency of HGF-1 cells. The cytotoxicity associated with the addition of Ag and the cell-conductive capacity associated with the addition of Zn are proportional to the added concentration, from 100 to 10,000 ppm. The dosages of both Ag(+) and Zn(2+) ions that should be added to HAp coatings were considered to prevent infection and improve biocompatibility. The results of this study ensure that HAp coatings modified with a moderate amount of Ag/Zn efficiently resist microorganisms and improve biocompatibility.
authors.affiliation: Department of Materials Science and Engineering, National Tsing Hua University, Hsinchu 300, Taiwan, Republic of China.
authors.forename: Ren-Jei
authors.initials: RJ
authors.lastname: Chung
authors.forename: Ming-Fa
authors.initials: MF
authors.lastname: Hsieh
authors.forename: Chine-Wen
authors.initials: CW
authors.lastname: Huang
authors.forename: Li-Hsiang
authors.initials: LH
authors.lastname: Perng
authors.forename: Hsiao-Wei
authors.initials: HW
authors.lastname: Wen
authors.forename: Tsung-Shune
authors.initials: TS
authors.lastname: Chin
mesh_terms: D001672:Biocompatible Materials; D005782:Gels; D005881:Gingiva / Q000187:drug effects*; D006801:Humans; D006882:Hydroxyapatites; D008855:Microscopy, Electron, Scanning
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D006882:Hydroxyapatites