TaqMan applications in genetic and molecular toxicology.
Quantification of nucleic acids has become a common procedure in many toxicology laboratories. Among the technologies that accomplish this is the fluorogenic 5'-nuclease assay, commonly known as TaqMan. Three TaqMan applications for genetic and molecular toxicology are presented in this article: quantification of gene expression, detection of genetic polymorphisms, and quantification of chromosomal DNA deletions. Of these, quantification of gene expression is the most widely used, and established TaqMan as a benchmark technology for nucleic acid quantification. Two additional applications, polymorphism detection and quantification of DNA deletions, demonstrate the flexibility and quantitative strengths that make TaqMan so powerful, including high precision, excellent sensitivity, and broad linear dynamic range. These and similar applications improve our ability to investigate genetic and molecular dimensions of toxicological phenomena, and have promoted the widespread use of TaqMan in toxicology departments in the pharmaceutical industry. In addition to presenting these applications, the authors discuss some of the challenges of integrating TaqMan and other new technologies into the drug development process.
Watson
David E
DE
Investigative Toxicology, Toxicology and Drug Disposition, A Division ofEli Lilly and Company, Greenfield, Indiana 46140, USA. Watson_David_E@Lilly.com
Li
Baohui
B
D000818:Animals; D017931:DNA Primers / Q000032:analysis; D004345:Drug Industry; D015870:Gene Expression / Q000187:drug effects; D009152:Mutagenicity Tests / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers