title: Intrastromal invasion by limbal epithelial cells is mediated by epithelial-mesenchymal transition activated by air exposure.
abstract: Corneal epithelial stem cells are located in the basal layer of the limbus between the cornea and the conjunctiva. Regulation of these limbal epithelial progenitor cells by the stromal niche dictates corneal surface health. To further characterize this process, limbal explants were cultured at the air-fluid interface, termed air-lifting, to stimulate the niche. As compared to submerged cultures, air-lifting significantly promoted epithelial stratification, migration, proliferation, and intrastromal invasion by limbal epithelial cells. Epithelial intrastromal invasion was noted when the limbal, but not corneal, epithelium was recombined with the limbal stroma containing live, but not dead, cells. Invading limbal basal cells displayed up-regulated nuclear expression of p63 and Ki67, down-regulated E-cadherin and cornea-specific keratin 3, and switched expression of beta-catenin from intercellular junctions to the nucleus and cytoplasm, indicating the activation of the Wnt/beta-catenin pathway. Invaded cells isolated by collagenase from the stroma of air-lifted, but not submerged, explants showed vivid clonal growth on 3T3 fibroblast feeder layers and complete epithelial-mesenchymal transition by expressing nuclear p63 and cytoplasmic S100A4. These findings collectively suggest that epithelial-mesenchymal transition via the Wnt/beta-catenin pathway influences the fate of limbal epithelial cells, likely to be progenitor cells, between regeneration and fibrosis when the stromal niche is activated.
authors.affiliation: TissueTech, Inc., Ocular Surface Center, 7000 SW 97 Ave., Suite 213, Miami, FL 33173, USA.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kawakita
authors.forename: Edgar M
authors.initials: EM
authors.lastname: Espana
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Chia-Yiang
authors.initials: CY
authors.lastname: Liu
authors.forename: Scheffer C G
authors.initials: SC
authors.lastname: Tseng
mesh_terms: D000388:Air; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D003319:Corneal Stroma / Q000378:metabolism / Q000473:pathology*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D019573:Epithelium, Corneal / Q000378:metabolism / Q000473:pathology*; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007633:Keratins / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D016850:Limbus Corneae / Q000378:metabolism / Q000473:pathology*; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism; D011817:Rabbits; D015534:Trans-Activators / Q000378:metabolism; D051153:Wnt Proteins; D051176:beta Catenin
chemical_list: C495267:CTNNB1 protein, mouse; D015820:Cadherins; D003598:Cytoskeletal Proteins; D036341:Intercellular Signaling Peptides and Proteins; D019394:Ki-67 Antigen; D010750:Phosphoproteins; D015534:Trans-Activators; C486419:Trp63 protein, mouse; D051153:Wnt Proteins; D051176:beta Catenin; D007633:Keratins