Crystal structure of Methanobacterium thermoautotrophicum phosphoribosyl-AMP cyclohydrolase HisI.
The metabolic pathway for histidine biosynthesis is interesting from an evolutionary perspective because of the diversity of gene organizations and protein structures involved. Hydrolysis of phosphoribosyl-AMP, the third step in the histidine biosynthetic pathway, is carried out by PR-AMP cyclohydrolase, the product of the hisI gene. The three-dimensional structure of PR-AMP cyclohydrolase from Methanobacterium thermoautotrophicum was solved and refined to 1.7 A resolution. The enzyme is a homodimer. The position of the Zn(2+)-binding site that is essential for catalysis was inferred from the positions of bound Cd(2+) ions, which were part of the crystallization medium. These metal binding sites include three cysteine ligands, two from one monomer and the third from the second monomer. The enzyme remains active when Cd(2+) is substituted for Zn(2+). The likely binding site for Mg(2+), also necessary for activity in a homologous cyclohydrolase, was also inferred from Cd(2+) positions and is comprised of aspartic acid side chains. The putative substrate-binding cleft is formed at the interface between the two monomers of the dimer. This fact, combined with the localization of the Zn(2+)-binding site, indicates that the enzyme is an obligate dimer.
Sivaraman
J
J
Biotechnology Research Institute, National Research Council, Montreal, Quebec H4P 2R2, Canada, Montreal.
Myers
Rebecca S
RS
Boju
Lorena
L
Sulea
Traian
T
Cygler
Miroslaw
M
Jo Davisson
V
V
Schrag
Joseph D
JD
D000595:Amino Acid Sequence; D000619:Aminohydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019843:Archaeal Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D002104:Cadmium / Q000737:chemistry; D018360:Crystallography, X-Ray; D019281:Dimerization; D006639:Histidine / Q000096:biosynthesis* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D017014:Methanobacterium / Q000201:enzymology* / Q000235:genetics; D017017:Methanococcus / Q000201:enzymology; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015032:Zinc / Q000378:metabolism
D019843:Archaeal Proteins; D002104:Cadmium; D006639:Histidine; D000619:Aminohydrolases; C022383:phosphoribosyl-AMP cyclohydrolase; D008274:Magnesium; D015032:Zinc