Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000.
The complete genomic sequence of Pseudomonas syringae pv. syringae B728a (Pss B728a) has been determined and is compared with that of P. syringae pv. tomato DC3000 (Pst DC3000). The two pathovars of this economically important species of plant pathogenic bacteria differ in host range and other interactions with plants, with Pss having a more pronounced epiphytic stage of growth and higher abiotic stress tolerance and Pst DC3000 having a more pronounced apoplastic growth habitat. The Pss B728a genome (6.1 Mb) contains a circular chromosome and no plasmid, whereas the Pst DC3000 genome is 6.5 mbp in size, composed of a circular chromosome and two plasmids. Although a high degree of similarity exists between the two sequenced Pseudomonads, 976 protein-encoding genes are unique to Pss B728a when compared with Pst DC3000, including large genomic islands likely to contribute to virulence and host specificity. Over 375 repetitive extragenic palindromic sequences unique to Pss B728a when compared with Pst DC3000 are widely distributed throughout the chromosome except in 14 genomic islands, which generally had lower GC content than the genome as a whole. Content of the genomic islands varies, with one containing a prophage and another the plasmid pKLC102 of Pseudomonas aeruginosa PAO1. Among the 976 genes of Pss B728a with no counterpart in Pst DC3000 are those encoding for syringopeptin, syringomycin, indole acetic acid biosynthesis, arginine degradation, and production of ice nuclei. The genomic comparison suggests that several unique genes for Pss B728a such as ectoine synthase, DNA repair, and antibiotic production may contribute to the epiphytic fitness and stress tolerance of this organism.
Feil
Helene
H
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.
Feil
William S
WS
Chain
Patrick
P
Larimer
Frank
F
DiBartolo
Genevieve
G
Copeland
Alex
A
Lykidis
Athanasios
A
Trong
Stephen
S
Nolan
Matt
M
Goltsman
Eugene
E
Thiel
James
J
Malfatti
Stephanie
S
Loper
Joyce E
JE
Lapidus
Alla
A
Detter
John C
JC
Land
Miriam
M
Richardson
Paul M
PM
Kyrpides
Nikos C
NC
Ivanova
Natalia
N
Lindow
Steven E
SE
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D001482:Base Composition; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D039002:Prophages / Q000235:genetics; D044224:Pseudomonas syringae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial