Post mortem degradation of nucleosides in the brain: comparison of human and rat brains for estimation of in vivo concentration of nucleosides.
There is an increasing attention paid for nucleoside metabolism and changes of nucleoside concentrations in human brain because of its pathological and physiological relevance. In order to determine the post mortem degradation of nucleosides and nucleoside metabolites, the concentrations of four nucleosides and three nucleobases were measured in rat and neurosurgical human cerebral cortical samples with 30s to 24h post mortem delay. Adenosine degradation coefficient (a multiplying factor for calculating concentrations of investigated substances for the living state) was 0.886 for human brain at 2 h post mortem time, while it was 1.976 for rats. Hypoxanthine, an adenosine degradation product had coefficients 0.564 for human brain and 0.812 for the rat brain. We provide data and degradation coefficients for the concentrations of adenosine, guanosine, inosine, uridine, uracil, hypoxanthine and xanthine with 2, 4, 6 and 24 h post mortem delay. We also report a method how to validate human neurosurgical brain samples in terms of sample preparation and statistical analysis.
Kovács
Zsolt
Z
Department of Zoology, Berzsenyi Dániel College, Szombathely, Károlyi Gáspár tér 4., 9700, Hungary.
Kékesi
Katalin Adrienna
KA
Bobest
Mátyás
M
Török
Tamás
T
Szilágyi
Nóra
N
Szikra
Tamás
T
Szepesi
Zsuzsanna
Z
Nyilas
Rita
R
Dobolyi
Arpád
A
Palkovits
Miklós
M
Juhász
Gábor
G
D000368:Aged; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009705:Nucleosides / Q000145:classification / Q000378:metabolism*; D011180:Postmortem Changes; D051381:Rats; D017208:Rats, Wistar
D009705:Nucleosides