Cloning and characterization of Munc18c(L), a novel murine Munc18c gene paralog.
We have identified and characterized a new mouse gene sequence, Munc18c(L), that appears closely related to the syntaxin-binding protein, Munc18c. The novel Munc18c(L) gene is comprised of 2 exons separated by a 600bp intron sequence with non-consensus donor and acceptor sites. Exons 1 and 2 of Munc18c(L) overlap with exons 1 through half of 9 of the Munc18c gene. The deduced amino acid sequence of Munc18c(L) is 271 amino acids long with homology to Munc18c protein ending at position 250. RT-PCR of murine tissues showed expression of Munc18c(L) in various tissues. RT-PCR carried out with a primer spanning the ATG codon and another one specific for the exon 2 of Munc18c(L) revealed two different transcripts of 0.8 and 1.4kbp in length. Using 5'-RACE, the start of Munc18c(L) exon 1 matches the one predicted for Munc18c, but the proximal promoter differ. This first identification of Munc18c(L) is vital in differentiating between Munc18c(L) and Munc18c and their potential roles in insulin-mediated glucose uptake.
Schlaepfer
Isabel R
IR
Division of Endocrinology, Metabolism and Diabetes, University of Colorado at Denver and Health Sciences Center, Aurora, CO, USA.
Pulawa
Leslie K
LK
Eckel
Robert H
RH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D051938:Munc18 Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014018:Tissue Distribution
D051938:Munc18 Proteins; C511447:Munc18c(L) protein, mouse