title: Determination of diarylheptanoids from Alpinia officinarum (Lesser Galangal) by HPLC with photodiode array and electrochemical detection.
abstract: Normal-phase column chromatography followed by semi-preparative reversed-phase HPLC has been used to isolate, from the rhizomes of Alpinia officinarum, five diarylheptanoids identified as 5-hydroxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone, 5-methoxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone, 7-(4"-hydroxyphenyl)-1-phenylhept-4-en-3-one, 7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-hept-4-en-3-one, 1,7-diphenylhept-4-en-3-one. The levels of these five diarylheptanoids in root material were determined quantitatively by HPLC with UV detection and the assay methods so developed were simple, rapid and accurate. Four of the diarylheptanoids could also be detected by HPLC with electrochemical detection (ECD) in the oxidative mode, and ECD was found to have a higher sensitivity than photodiode array detection.
authors.affiliation: Food Science Department and Center for Advanced Food Technology, Cook College, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Liu
authors.forename: Shengmin
authors.initials: S
authors.lastname: Sang
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Hartman
authors.forename: Chi-Tang
authors.initials: CT
authors.lastname: Ho
authors.forename: Robert T
authors.initials: RT
authors.lastname: Rosen
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D006536:Heptanes / Q000302:isolation & purification*; D007659:Ketones / Q000302:isolation & purification*; D013058:Mass Spectrometry; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D027343:Rhizome / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004365:Drugs, Chinese Herbal; D006536:Heptanes; D007659:Ketones