title: [Expression and antigenicity analysis of p46000 antigen from newborn larvae of Trichinella spiralis].
abstract: OBJECTIVE
  To express and analyze p46000 antigen from newborn larvae of Trichinella spiralis.
  
  
  METHODS
  p46000 antigen gene was subcloned to the pET28a expression system by PCR. The recombinant transformant was induced by IPTG and the antigenicity was analyzed with ELISA and Western blotting.
  
  
  RESULTS
  The molecular weight of the expressed protein was about Mr 48000. ELISA and Western blotting showed that this recombinant protein could be recognized by T. spiralis infected swine serum and rabbit anti-recombinant protein serum, and the rabbit anti-recombinant protein serum could recognize a Mr 46000 protein from newborn larvae of T. spiralis.
  
  
  CONCLUSIONS
  p46000 recombinant antigen from newborn larvae of T. spiralis was expressed which shows a specific antigenicity.
authors.affiliation: Faculty of Military Veterinary Quartermaster University of PLA, Changchun 130062, China.
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Yuan
authors.forename: Bao-quan
authors.initials: BQ
authors.lastname: Fu
authors.forename: Ming-yuan
authors.initials: MY
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Ya-lan
authors.initials: YL
authors.lastname: Zhang
authors.forename: Xiu-ping
authors.initials: XP
authors.lastname: Wu
authors.forename: Ben-fu
authors.initials: BF
authors.lastname: Lin
authors.forename: Lian-rui
authors.initials: LR
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.forename: Qi-jun
authors.initials: QJ
authors.lastname: Chen
authors.forename: Pascal
authors.initials: P
authors.lastname: Boireau
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis* / Q000276:immunology; D015870:Gene Expression; D007106:Immune Sera; D007814:Larva / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D013552:Swine; D017160:Trichinella spiralis / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D007106:Immune Sera; D011994:Recombinant Proteins