title: Protective effect of propofol against kainic acid-induced lipid peroxidation in mouse brain homogenates: comparison with trolox and melatonin.
abstract: This study compared the effectiveness of propofol with that of trolox and melatonin for reduction of lipid peroxidation in vitro. Lipid peroxidation was induced by addition of kainic acid (KA; 10 mM), hydrogen peroxide (H2O2; 10 mM), or ferrous ammonium sulfate (5 microM) to mouse brain homogenate, and thiobarbituric acid-reactive substances (TBA-RS) were used as a marker of lipid peroxidation. Propofol, trolox, and melatonin reduced KA-, H2O2-, and ferrous ammonium sulfate-induced lipid peroxidation in a concentration-dependent manner. In reducing KA-induced lipid peroxidation, 50% inhibitory concentration (IC50) values of antioxidants were as follows: propofol (11.33 mM), trolox (4.00 mM), and melatonin (9.72 mM). In reducing H2O2-induced lipid peroxidation, IC50 values of antioxidants were as follows: propofol (56.86 mM), trolox (33.34 mM), and melatonin (26.63 mM). In reducing ferrous ion-induced lipid peroxidation, IC50 values of antioxidants were as follows: propofol (49.57 mM), trolox (60.35 mM), and melatonin (22.02 mM). Under the in vitro conditions of this experiment, propofol was an excellent and a very potent antioxidant in inhibiting KA-, H2O2-, and ferrous ion-induced lipid peroxidation in mouse brain homogenates. We conclude that the antioxidant properties of propofol at clinically relevant anesthetic concentrations may have a neuroprotective effect.
authors.affiliation: Department of Neurology, School of Medicine, Keimyung University, Taegu, South Korea.
authors.forename: Hyung
authors.initials: H
authors.lastname: Lee
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Jang
authors.forename: Seong-Ryong
authors.initials: SR
authors.lastname: Lee
mesh_terms: D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001923:Brain Chemistry / Q000187:drug effects*; D002839:Chromans / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000633:toxicity*; D005296:Ferrous Compounds / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008550:Melatonin / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D015742:Propofol / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D018686:Anesthetics, Intravenous; D000975:Antioxidants; D002839:Chromans; D018691:Excitatory Amino Acid Antagonists; D005296:Ferrous Compounds; D000644:Quaternary Ammonium Compounds; D017392:Thiobarbituric Acid Reactive Substances; C038178:ammonium ferrous sulfate; D006861:Hydrogen Peroxide; D008550:Melatonin; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; D007608:Kainic Acid; D015742:Propofol