Geographical distribution of genetic polymorphism of the pathogen Histoplasma capsulatum isolated from infected bats, captured in a central zone of Mexico.
Fourteen Histoplasma capsulatum isolates recovered from infected bats captured in Mexican caves and two human H. capsulatum reference strains were analyzed using random amplification of polymorphic DNA PCR-based and partial DNA sequences of four genes. Cluster analysis of random amplification of polymorphic DNA-patterns revealed differences for two H. capsulatum isolates of one migratory bat Tadarida brasiliensis. Three groups were identified by distance and maximum-parsimony analyses of arf, H-anti, ole, and tub1 H. capsulatum genes. Group I included most isolates from infected bats and one clinical strain from central Mexico; group II included the two isolates from T. brasiliensis; the human G-217B reference strain from USA formed an independent group III. Isolates from group II showed diversity in relation to groups I and III, suggesting a different H. capsulatum population.
Taylor
Maria Lucia
ML
Laboratorio de Inmunología de Hongos, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria. emello@servidor.unam.mx
Chávez-Tapia
Catalina B
CB
Rojas-Martínez
Alberto
A
del Rocio Reyes-Montes
Maria
M
del Valle
Mirian Bobadilla
MB
Zúñiga
Gerardo
G
D000818:Animals; D002685:Chiroptera / Q000382:microbiology*; D005656:Fungal Proteins / Q000235:genetics; D006658:Histoplasma / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006660:Histoplasmosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012015:Reference Standards; D017422:Sequence Analysis, DNA
D005656:Fungal Proteins