Detection of phosphatidylcholine oxidation products in rat heart using quadrupole time-of-flight mass spectrometry.
An improved technique for the analysis of phosphatidylcholine (PC) and lyso-phosphatidylcholine (lyso-PC) oxidation products was developed using quadrupole time of flight (Q-TOF) mass spectrometry with electrospray ionization. We separated these products using an HPLC C(8) column with a gradient of methanol and 10mM aqueous ammonium acetate. Monohydroxides, oxo derivatives, and trihydroxides of palmitoyl-linoleoyl (C16:0/C18:2) PC, stearoyl-linoleoyl (C18:0/C18:2) PC, and oleoyl-linoleoyl (C18:1/C18:2) PC were detected mainly as MH(+) and [M+Na](+) ions in the heart of the intact rat. Using standard synthetic PC-OH (C16:0/C18:2-OH), the lipid extract component was identified as (C16:0/C18:2-OH) PC based on the product ions of ESI-MS-MS and, the PC-OH concentration was quantitated. Four oxidatively modified 1-lyso-phosphatidylcholines (lyso-PCs) were also detected. This is the first report showing the presence of monohydroxides, oxo derivatives, and trihydroxides of (C16:0/C18:2)PC, (C18:0/C18:2)PC, and (C18:1/C18:2) PC in the rat heart.
Adachi
Junko
J
Department of Legal Medicine, Kobe University Graduate School of Medicine, 7 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. adachi@med.kobe-u.ac.jp
Yoshioka
Naoki
N
Sato
Mariko
M
Nakagawa
Kanako
K
Yamamoto
Yorihiro
Y
Ueno
Yasuhiro
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D008054:Lipid Peroxides / Q000032:analysis; D008244:Lysophosphatidylcholines / Q000378:metabolism; D009206:Myocardium / Q000737:chemistry*; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000378:metabolism*; D051381:Rats; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D005231:Fatty Acids, Unsaturated; D008054:Lipid Peroxides; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; C006065:We 201