title: A high-throughput 3-D composite dielectrophoretic separator.
abstract: Dielectrophoresis has great potential to offer a range of diverse fields from bioprocessing to clinical medicine, but is hampered by low throughput rates to the micrometer scale of the electrodes required to generate highly nonuniform fields. Here we describe a novel approach to electrode construction, using a drilled laminated structure to form channels bearing electrodes 30 microm across and 150 microm apart. Since these electrodes appear along all sides of the drilled bore, the trapping efficiency is improved over conventional devices. We have developed and demonstrated a separator capable of sorting a 50:50 mixture of viable and nonviable yeast cells into an 86:14 mixture at 25 mLhr(-1).
authors.affiliation: School of Engineering, University of Surrey, Surrey GU2 7XH, UK.
authors.forename: Henry O
authors.initials: HO
authors.lastname: Fatoyinbo
authors.forename: David
authors.initials: D
authors.lastname: Kamchis
authors.forename: Reginald
authors.initials: R
authors.lastname: Whattingham
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Ogin
authors.forename: Michael P
authors.initials: MP
authors.lastname: Hughes
mesh_terms: D003585:Cell Biology; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D003198:Computer Simulation; D017076:Computer-Aided Design; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008904:Miniaturization; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification*