The effect of serum from women with preeclampsia on JAR (trophoblast-like) cell line.
OBJECTIVE
Pathologic placentation has been implicated in the pathogenesis of preeclamsia. We sought to assess the effect serum obtained from women with preeclampsia would have on JAR human choriocarcinoma cells regarding growth, invasiveness, and matrix metalloproteinase (MMP) secretion as compared to normotensive pregnant woman.


METHODS
Blood was collected from 11 healthy pregnant women and from10 patients with preeclampsia at 28-33 weeks of gestation. The JAR human choriocarcinoma cell line was cultured in the presence of 10% serum obtained from each group. Cell proliferation, invasiveness, and MMP secretion was measured using a cell proliferation kit, the Matrigel (BD Biosciences, Beit-Ha'Emek, Israel) invasion assay, and gel zymography, respectively.


RESULTS
Cell growth increased by 6% when exposed to serum from patients with preeclampsia compared to 30% from controls (P <.01). Trophoblast invasion was significantly (P <.01) reduced in the preeclampsia group (21 +/- 1.9%) compared to controls (27 +/- 2.5%). Valid MMP-2 secretion was reduced by 51% in the preeclampsia group compared to controls (P <.05).


CONCLUSIONS
Serum obtained from women with preeclampsia contains a factor or factors that exhibit an inhibitory effect on JAR trophoblast cell proliferation, invasiveness, and MMP-2 secretion. These factors may be involved in the pathologic placentation associated with the pathogenesis of preeclampsia.
Mahameed
Safa
S
Laboratory for Research in Reproductive Sciences, Department of Obstetrics and Gynecology, Ha'Emek Medical Center, Afula, Israel.
Goldman
Shlomit
S
Gabarin
Diane
D
Weiss
Amir
A
Shalev
Eliezer
E
D000328:Adult; D016022:Case-Control Studies; D049109:Cell Proliferation; D002822:Choriocarcinoma / Q000473:pathology; D005260:Female; D006801:Humans; D020782:Matrix Metalloproteinases / Q000378:metabolism; D009361:Neoplasm Invasiveness; D011225:Pre-Eclampsia / Q000503:physiopathology*; D011247:Pregnancy; D044967:Serum / Q000737:chemistry*; D014327:Trophoblasts; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000473:pathology
D020782:Matrix Metalloproteinases