title: [Nuclear localization region in soluble adenylyl cyclase].
abstract: OBJECTIVE
  To locate the region responsible for nuclear localization of protein sAC.
  
  
  METHODS
  The eukaryotic expression vector of vairous sAC deletion mutants were transfected into Hela cells. The localization of each mutant was observed using confocal microscope.
  
  
  RESULTS
  For some mutants, the localization of sAC changed. Deletion of some region made it unable to locate in the nuclear.
  
  
  CONCLUSIONS
  It is possible to figure out that the nucleotide region (739-1038 and 1045-1261) take charge of nuclear localization of sAC.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
authors.forename: Qi-ping
authors.initials: QP
authors.lastname: Feng
authors.forename: Jin
authors.initials: J
authors.lastname: Zuo
authors.forename: Yan
authors.initials: Y
authors.lastname: Meng
authors.forename: Fu-de
authors.initials: FD
authors.lastname: Fang
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005820:Genetic Testing; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D009687:Nuclear Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D009687:Nuclear Proteins; D000262:Adenylyl Cyclases