Effects of a 50 Hz electric field on plasma lipid peroxide level and antioxidant activity in rats.
The effects of exposure to extremely low frequency electric fields (ELF EFs) on plasma lipid peroxide levels and antioxidant activity (AOA) in Sprague-Dawley rats were studied. The test was based on comparisons among rats treated with a combination of the oxidizing agent, 2,2'-azobis(2-aminopropane) dihydrochloride (AAPH) and 50 Hz EF of 17.5 kV/m intensity for 15 min per day for 7 days, AAPH alone, EF alone or no treatment. EF significantly decreased the plasma peroxide level in rats treated with AAPH, similar to treatment by ascorbic acid or the superoxide dismutase. Ascorbic acid increased AOA; however, EF and superoxide dismutase did not change AOA compared with sham exposure in stressed rats. No influence on the lipid peroxide level and AOA in unstressed rats was observed with EF exposure alone. Although the administration of AAPH decreased AOA, this decrease did not change when EF was added. These data indicate that the ELF EF used in this study influenced the lipid peroxide level in an oxidatively stressed rat.
Harakawa
Shinji
S
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan. harakawa@bd5.so-net.ne.jp
Inoue
Noboru
N
Hori
Takuya
T
Tochio
Koji
K
Kariya
Tatsuya
T
Takahashi
Kunihito
K
Doge
Fuyuki
F
Suzuki
Hiroshi
H
Nagasawa
Hideyuki
H
D000578:Amidines / Q000008:administration & dosage; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004560:Electricity; D004574:Electromagnetic Fields; D004781:Environmental Exposure; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage; D051381:Rats; D017207:Rats, Sprague-Dawley
D000578:Amidines; D000975:Antioxidants; D008054:Lipid Peroxides; C046728:2,2'-azobis(2-amidinopropane)