[Effective cryopreservation of human embryonic stem cells by programmed freezing].
Cryopreservation of human embryonic stem cells is an important and unsolved problem. A computer-controlled programmable cooler was used in the preservation of ES cells. Several effects have been experimentally studied, which include the cooling rates, the temperature of seeding, the temperatures before the samples being plunged into liquid nitrogen, and the cryoprotective agents. It was found that the favorable constitution of cryoprotective agents was Me2SO+ FBS+DMEM(1:3:6, v/v/v) with cooling protocol of -0.5 degrees C/min from 0 degrees C to -35 degrees C (seeding at -10 degrees C), and being plunged into the liquid nitrogen immediately. The high survival rate (81.8%) was obtained.
Yang
Peng Fei
PF
Institute of Cryomedicine and Cryogenics, Shanghai University of Science and Technology, Shanghai 200093.
Tsung
Hsiao Chien
HC
Cheng
Qi Kang
QK
Hua
Tse Chao
TC
Wu
Chun Fang
CF
Cao
Yi Lin
YL
D002460:Cell Line; D015925:Cryopreservation / Q000379:methods*; D053595:Embryonic Stem Cells; D006801:Humans