Detection of Nepsilon-monomethyllysine using high-performance liquid chromatography and high-performance liquid chromatography-mass spectrometry.
Nepsilon-Monomethyllysine was identified in the serum, urine, brain, and liver samples of rats treated per os with L-deprenyl. The identification procedure included reaction with Fmoc chloride, clean-up, and analysis using HPLC-UV-MS. Oral administration of (-)-N-14C-methyl-N-propynyl(2-phenyl-1-methyl)ethylammonium hydrochloride L-deprenyl) to rats resulted in transfer of the radiolabelled methyl group to the Nepsilon-amino group of the endogenous lysine. The radiolabelled Nepsilon-monomethyllysine was urinary eliminated together with the other radiolabelled deprenyl metabolites, such as deprenyl-N-oxide and methamphetamine. The presence of Nepsilon-monomethyllysine has also been traced, and its concentrations were compared in the serum, liver and brain of rats subjected to L-deprenyl treatment. Methyl group transfer from the L-deprenyl to endogenous compounds; and the urinary elimination of their products may offer a vital way to eliminate or to decrease the degree of drug transmethylation to the lysine constituents of blood vessels' proteins.
Kalász
H
H
Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, H-1089 Budapest, Hungary. Huba.kalasz@gmail.com
Szücs
Z
Z
Tihanyi
M
M
Szilágyi
A
A
Lengyel
J
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
C005205:epsilon-N-methyllysine; D008239:Lysine