[Expression and antigenicity analysis of p46000 antigen from newborn larvae of Trichinella spiralis].
OBJECTIVE
To express and analyze p46000 antigen from newborn larvae of Trichinella spiralis.


METHODS
p46000 antigen gene was subcloned to the pET28a expression system by PCR. The recombinant transformant was induced by IPTG and the antigenicity was analyzed with ELISA and Western blotting.


RESULTS
The molecular weight of the expressed protein was about Mr 48000. ELISA and Western blotting showed that this recombinant protein could be recognized by T. spiralis infected swine serum and rabbit anti-recombinant protein serum, and the rabbit anti-recombinant protein serum could recognize a Mr 46000 protein from newborn larvae of T. spiralis.


CONCLUSIONS
p46000 recombinant antigen from newborn larvae of T. spiralis was expressed which shows a specific antigenicity.
Yuan
Li-hong
LH
Faculty of Military Veterinary Quartermaster University of PLA, Changchun 130062, China.
Fu
Bao-quan
BQ
Liu
Ming-yuan
MY
Gao
Fei
F
Zhang
Ya-lan
YL
Wu
Xiu-ping
XP
Lin
Ben-fu
BF
Li
Lian-rui
LR
Lu
Qiang
Q
Chen
Qi-jun
QJ
Boireau
Pascal
P
D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis* / Q000276:immunology; D015870:Gene Expression; D007106:Immune Sera; D007814:Larva / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D013552:Swine; D017160:Trichinella spiralis / Q000276:immunology*
D000947:Antigens, Helminth; D007106:Immune Sera; D011994:Recombinant Proteins