[Differentiation-inducing effects of perphenazine on K562 leukemia cells].
OBJECTIVE
To determine the differentiation-inducing effects of perphenazine on K562 leukemia cells.


METHODS
Differentiation-Inducing effects of a phenothiazine perphenazine were evaluated by proliferation, morphology and function of K562 cells. We evaluated the effects of perphenazine on K562 cells proliferation by cellular enumeration in liquid culture assay, MTT assay and clony formation assay, the morphology by Wight-Gimesa staining, and the function by detecting CD71 through flow cytometry.


RESULTS
Perphenazine enhanced the expression of CD71 on K562 cells and increased Hb content in K562 cells, while inhibited the proliferation of K562 cells. K562 cells showed differentiation morphology after the drug treatment.


CONCLUSIONS
Perphenazine possessed differentiation-inducing effects on K562 cells.
Yuan
Lin-Bo
LB
Research Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
He
Qun
Q
D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D010546:Perphenazine / Q000494:pharmacology*
D010546:Perphenazine