title: Hybrid nanogels with physical and chemical cross-linking structures as nanocarriers.
abstract: Polymerizable nanogels were prepared by self-assembly of cholesteryl group-bearing pullulan (CHP) with methacryloyl groups (CHPMA). The CHPMA nanogel was polymerized with 2-methacryloyloxyethyl phosphorylcholine (MPC) by radical polymerization in dilute aqueous solution. The solution properties of the polymers in water were investigated by TEM, SEC-MALS, and fluorescence quenching technique. Monodispersed hybrid nanogels of CHPMA-MPC (CM nanogels) (25-30 nm in radius of gyration) were obtained by using CHPMA nanogel as a seed-nanogel. CM nanogels have a dual cross-linking structure that is physically cross-linked with the cholesteryl groups and chemically cross-linked with the MPC polymer chains. CM nanogels trap heat-denatured carbonic anhydrase B (CAB) and prevent their aggregations. The nanogels maintained the ability of trapping and releasing enzymes by host-guest interaction of cholesteryl group and cyclodextrin.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10, Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Morimoto
authors.forename: Takao
authors.initials: T
authors.lastname: Endo
authors.forename: Michiko
authors.initials: M
authors.lastname: Ohtomi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Kazunari
authors.initials: K
authors.lastname: Akiyoshi
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D005936:Glucans; D020100:Hydrogels; D008689:Methacrylates; D011108:Polymers; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; C009109:pullulan; D002784:Cholesterol