KIAA0649, a 1A6/DRIM-interacting protein with the oncogenic potential.
We identified a 1A6/DRIM (down-regulated in metastasis) interacting protein, KIAA0649 during the yeast two-hybrid screen. The interaction between KIAA0649 and 1A6/DRIM was further confirmed by GST-pull-down and co-immunoprecipitation assays. KIAA0649 was originally identified from human brain tissue. However, its biological function remains unknown. In this report, we showed that KIAA0649 mRNA is widely expressed in human multiple tissues and cell lines. We have also demonstrated that KIAA0649 has oncogenic characteristics: it enhances colony formation, allows anchorage-independent growth. Moreover, KIAA0649 exogenous expression in NIH3T3 fibroblasts caused tumor development in nude mice.
Yang
Lin
L
Beijing Institute for Cancer Research, School of Oncology, Peking University No. 1 Da Hong Luo Chang St, West District, Beijing 100034, PR China.
Zhao
Jun
J
Lü
Wenqing
W
Li
Yong
Y
Du
Xiaojuan
X
Ning
Tao
T
Lu
Guirong
G
Ke
Yang
Y
D000818:Animals; D019556:COS Cells; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002522:Chlorocebus aethiops; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D041681:NIH 3T3 Cells; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009687:Nuclear Proteins; D015513:Oncogene Proteins / Q000378:metabolism*; D054645:Protein Phosphatase 1; D011506:Proteins / Q000378:metabolism*; D012261:Ribonucleoproteins; D020798:Two-Hybrid System Techniques
D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D015513:Oncogene Proteins; C511445:PPP1R26 protein, human; D011506:Proteins; D012261:Ribonucleoproteins; C113527:UTP20 protein, human; D054645:Protein Phosphatase 1