[Design and improvement of an elective culture medium for bacteriological diagnosis of Salmonella infection].
Experimental studies have provided a nutrient broth for elective accumulation of Salmonella (MA broth) and a differential diagnostic culture medium for selective isolation of the causative agents of Salmonella toxic infections. The MA broth is used for bacteriological isolation of salmonellae from the low-infected material and for a sanitary-and-bacteriological study of meat-and- sausage and other foods for this pathogen. A combination of high accumulating and inhibiting properties of the designed broth is its main advantage of over its foreign analogue (Muller's tetrathionate broth). The differential diagnostic culture medium for selective isolation of salmonellae (analogue of brilliant grun-agar) has a high sensitivity to the bacteria of the genus Salmonella, such as S. typhimurium, S. enteriditis, and S. london, that are causative agents of food toxic infections and form transparent bright-pink colonies on the medium. The growth of microbial associates, such as Escherichia coli, Proteus, Shigella, pathogens of a typhoid-and-paratyphoid group, is considerably suppressed. Bacteria of the genus Klebsiella are well differentiated as transparent bright-yellow colonies.
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods; D003470:Culture Media; D006801:Humans; D012475:Salmonella / Q000254:growth & development* / Q000302:isolation & purification; D012480:Salmonella Infections / Q000175:diagnosis / Q000382:microbiology*
D003470:Culture Media