The nadA gene of Pseudomonas fluorescens PGPR strain 267.1.
An insertion mutant of Pseudomonas fluorescens PGPR strain 267.1 was found to be auxotrophic for niacin (nicotinic acid) and could not synthesize quinolinic acid. The Tn5 interrupted gene was cloned and sequenced. The cloned fragment contained an open reading frame, nadA, capable of encoding a 359-amino-acid protein (39.0 kDa) with substantial identity to various bacterial quinolinate synthetases. The nadA gene complemented quinolinic acid synthesis deficiency and niacin auxotrophy of the P. fluorescens 106 P nadA mutant.
Marek-Kozaczuk
Monika
M
Department of General Microbiology, University of Maria Curie-Skłodowska, Akademicka 19 st, Lublin 20-033, Poland.
Rogalski
Jerzy
J
Skorupska
Anna
A
D005798:Genes, Bacterial; D009097:Multienzyme Complexes / Q000235:genetics*; D009525:Niacin / Q000378:metabolism*; D010802:Phylogeny; D011551:Pseudomonas fluorescens / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D011805:Quinolinic Acids / Q000378:metabolism*
D009097:Multienzyme Complexes; D011805:Quinolinic Acids; D009525:Niacin; C022512:quinolinic acid synthetase