Apoptosis and biochemical biomarkers of stress in spiders from industrially polluted areas exposed to high temperature and dimethoate.
The aim of this study was to evaluate the relations between apoptosis and the activity of antioxidant enzymes (superoxide dismutase; catalase) and quantitative changes in stress protein positive cells (Hsp70; metallothionein) in midgut glands of funnel web spiders Agelena labyrinthica (Agelenidae) and wolf spiders Pardosa lugubris (Lycosidae) exposed to high temperature and pesticide under laboratory conditions. The spiders were collected from two meadow ecosystems differently polluted with metals (Olkusz and Pilica, southern Poland). Under stress conditions, P. lugubris had fewer apoptotic cells in the midgut glands than A. labyrinthica. In P. lugubris from both sites, the observed increase in the percentage of metallothionein and Hsp70-positive cells, simultaneous with intensification of superoxide dismutase and catalase activity, suggests an anti-apoptotic function of those proteins in representatives of wandering spiders. In the midgut glands of A. labyrinthica, heat shock and dimethoate increased the number of Annexin V-positive cells as well as the amounts of mitochondria with low transmembrane potential (DeltaPsi(m)) versus the control. The changes in the percentage of MT- and Hsp70-positive cells in funnel web spiders were less than in wolf spiders. The absence of change in SOD and CAT activity in A. labyrinthica shows that the participation of those enzymes in antioxidant reactions is minimal in this species.
Wilczek
Grazyna
G
Department of Animal Physiology and Ecotoxicology Faculty of Biology and Environmental Protection, The University of Silesia, Bankowa 9, 40-007 Katowice, Poland. gwilczek@us.edu.pl
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015415:Biomarkers; D002374:Catalase / Q000378:metabolism; D004117:Dimethoate / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D006358:Hot Temperature; D007221:Industry; D007306:Insecticides / Q000633:toxicity*; D008668:Metallothionein / Q000032:analysis; D019216:Metals, Heavy / Q000633:toxicity*; D013112:Spiders / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D015415:Biomarkers; D004785:Environmental Pollutants; D018840:HSP70 Heat-Shock Proteins; D007306:Insecticides; D019216:Metals, Heavy; D008668:Metallothionein; D002374:Catalase; D013482:Superoxide Dismutase; D004117:Dimethoate