Association of fowl adenovirus serotype 12 with hydropericardium syndrome of poultry in India.
Eight fowl adenovirus (FAdV) isolates obtained from different geographical regions of India were typed by a virus-neutralization test (VNT) using rabbit antisera against all the 12 serotypes of FAdV and by PCR for the hyper-variable region of hexon gene combined with restriction fragment length polymorphism (RFLP) analysis using AluI and MboI restriction enzymes. It was found that six isolates belonged to FAdV-4, one to FAdV-12 and one to both of them. This study revealed the involvement of FAdV-12 alone or in association with FAdV-4 in precipitating inclusion body hepatitis--hydropericardium syndrome (IBH-HPS) among poultry flocks in the country.
Rahul
S
S
Division of Avian Diseases, Indian Veterinary Research Institute, Izatnagar--243122 (U.P.), India.
Kataria
J M
JM
Senthilkumar
N
N
Dhama
K
K
Sylvester
S A
SA
Uma
R
R
D000257:Adenoviridae Infections / Q000821:virology*; D000818:Animals; D000259:Aviadenovirus / Q000145:classification* / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D007194:India; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011200:Poultry; D011201:Poultry Diseases / Q000821:virology*; D012703:Serotyping
D004279:DNA, Viral; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases