The role of adsorbed fibrinogen in platelet adhesion to polyurethane surfaces: a comparison of surface hydrophobicity, protein adsorption, monoclonal antibody binding, and platelet adhesion.
Ten specially synthesized polyurethanes (PUs) were used to investigate the effects of surface properties on platelet adhesion. Surface composition and hydrophilicity, fibrinogen (Fg) and von Willebrand's factor (vWf) adsorption, monoclonal anti-Fg binding, and platelet adhesion were measured. PUs preadsorbed with afibrinogenemic plasma or serum exhibited very low platelet adhesion, while adhesion after preadsorption with vWf deficient plasma was not reduced, showing that Fg is the key plasma protein mediating platelet adhesion under static conditions. Platelet adhesion to the ten PUs after plasma preadsorption varied greatly, but was only partially consistent with Fg adsorption. Thus, while very hydrophilic PU copolymers containing PEG that had ultralow Fg adsorption also had very low platelet adhesion, some of the more hydrophobic PUs had relatively high Fg adsorption but still exhibited lower platelet adhesion. To examine why some PUs with high Fg adsorption had lower platelet adhesion, three monoclonal antibodies (mAbs) that bind to sites in Fg thought to mediate platelet adhesion were used. The antibodies were: M1, specific to gamma-chain C-terminal; and R1 and R2, specific to RGD containing regions in the alpha-chain N- and C-terminal, respectively. Platelet adhesion was well correlated with M1 binding, but not with R1 or R2 binding. When these mAbs were incubated with plasma preadsorbed surfaces, they blocked adhesion to variable degrees. The ability of the R1 and R2 mAbs to partially block adhesion to adsorbed Fg suggests that RGD sites in the alpha chain may also be involved in mediating platelet adhesion and act synergistically with the C-terminal of the gamma-chain.
Wu
Yuguang
Y
Department of Bioengineering, Box 351750, University of Washington, Seattle, Washington 98195, USA.
Simonovsky
Felix I
FI
Ratner
Buddy D
BD
Horbett
Thomas A
TA
D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D001672:Biocompatible Materials / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D010973:Platelet Adhesiveness; D011140:Polyurethanes / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties; D014841:von Willebrand Factor / Q000737:chemistry
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D011140:Polyurethanes; D014841:von Willebrand Factor; D005340:Fibrinogen