title: Development and validation of a method for the quantitative determination of aflatoxin contaminants in Maytenus ilicifolia by HPLC with fluorescence detection.
abstract: A method for the quantification of aflatoxins B1, G1, B2 and G2 in the medicinal herb Maytenus ilicifolia was developed and validated. The method used immunoaffinity columns for sample clean-up and HPLC with fluorescence detection without any derivatisation step. The method showed good inter-day accuracy (bias values in the range 4.5-10.7%) and precision (5-16% RSD) when applied to the determination of levels of aflatoxins ranging from 7 to 20 ppb in the plant material. The detection limits for samples of the plant material spiked with aflatoxins were 3.5 ng/g for B1 and G1 and 0.1 ng/g for B2 and G2. The method was successfully applied to commercial samples of Maytenus ilicifolia for the screening of aflatoxin contaminants.
authors.affiliation: UDEM/LTF, Universidade Federal da Paraíba, Caixa Postal 5044, CEP: 58051-970, João Pessoa, PB, Brazil.
authors.forename: Sayonara Maria Lia Fook Meira
authors.initials: SM
authors.lastname: Braga
authors.forename: Francinalva Dantas
authors.initials: FD
authors.lastname: de Medeiros
authors.forename: Eduardo Jesus
authors.initials: EJ
authors.lastname: de Oliveira
authors.forename: Rui Oliveira
authors.initials: RO
authors.lastname: Macedo
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D001938:Brazil; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029755:Maytenus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D000348:Aflatoxins; D010936:Plant Extracts