[Effects of Gardenia-aweto compound by different extraction method on antagonizing acute respiratory distress syndrome].
OBJECTIVE
To study the effect of Gardenia-Aweto compound (GAC) and two component on preventing acute respiratory distress syndrome (ARDS) by the rabbit model of ARDS induced by intravenous injection of oleic acid. To detect the efficiency component of GAC in preventing ARDS.


METHODS
GAC was divided into two compounts, ethanol-soluble components (ESC) and ethanol-deposition components (EDC), based on polarity. Forty-three new zealand rabbits were randomly divided into five groups, the blank control group, the model group, the GAC groups, the ESC group, and the EDC group. The ARDS model was induced by intravenous injection of oleic acid. Dynamic changes of arterial blood gas, lung index, albumin in bronchoalveolar lavage fluid (BALF) in different groups and lung histological changes were observed and compared.


RESULTS
As compared with the blank group, in the model group, GAC group, ESC group, EDC group the arterial PO2 and oxygen saturation deprived continuously. While SO2 in GAC group at time points 30, 60, 90, 120 min (P < 0.05 or 0.01) and SO2 in ESC group at time points 30, 60, 90 min were higher than those in ARDS group. PO2 in ESC group at time points 30, 60 min (P < 0.05) were higher than those in ARDS group. The value of LI and W/D were higher in ARDS group than in sham group (P < 0.01), they were much lower in HD group than in ARDS group (P < 0.01). Concentration of BALF-albumin increased markedly in ARDS group and pre-treatment groups compared with sham group, but it was much lower in GAC group and ESC group, there was a significant difference between GAC group (P < 0.01), ESC group (P < 0.05) and ARDS group. The lung histological changes had been improved in GAC group and ESC group. But no significantly difference between above-mentioned parameters was found in comparison in the model group and in the EDC group.


CONCLUSIONS
Preventive administration of GAC or ESC an protect the damaged lung function in ARDS rabbits induced by oleic acid. The efficiency component of GAC in preventing ARDS is ESC. GAC antagonizing ARDS may relate to its anti-inflammatory, immuno-modulatory, anti-oxidant and antithrombotic effects.
Yan
Dong
D
First Affiliated Hospital College of Medical Science Zhejiang University, Hangzhou 310003, China.
Li
Lan-juan
LJ
Du
Wei-bo
WB
Ren
Guo-ping
GP
Cao
Hong-cui
HC
Zhu
Di-feng
DF
Xia
Lei
L
Zeng
Zhen
Z
Shao
Qing
Q
Zhang
Hai-jiang
HJ
Chen
Yi-yu
YY
D000818:Animals; D032661:Cordyceps / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000627:therapeutic use*; D005260:Female; D032062:Gardenia / Q000737:chemistry; D007915:Lepidoptera; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000627:therapeutic use*; D019301:Oleic Acid; D008517:Phytotherapy; D011659:Pulmonary Gas Exchange; D011817:Rabbits; D011897:Random Allocation; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D008419:Materia Medica; D019301:Oleic Acid