title: An optimized isolation and labeling platform for accurate microRNA expression profiling.
abstract: MicroRNAs (miRNAs) are small, noncoding RNAs that regulate gene expression in both plants and animals. miRNA genes have been implicated in a variety of important biological processes, including development, differentiation, apoptosis, fat metabolism, viral infection, and cancer. Similar to protein-coding messenger RNAs, miRNA expression varies between tissues and developmental states. To acquire a better understanding of global miRNA expression in tissues and cells, we have developed isolation, labeling, and array procedures to measure the relative abundance of all of the known human mature miRNAs. The method relies on rapid isolation of RNA species smaller than ~40 nucleotides (nt), direct and homogenous enzymatic labeling of the mature miRNAs with amine modified ribonucleotides, and hybridization to antisense DNA oligonucleotide probes. A thorough performance study showed that this miRNA microarray system can detect subfemtomole amounts of individual miRNAs from <1 mug of total RNA, with 98% correlation between independent replicates. The system has been applied to compare the global miRNA expression profiles in 26 different normal human tissues. This comprehensive analysis identified miRNAs that are preferentially expressed in one or a few related tissues and revealed that human adult tissues have unique miRNA profiles. This implicates miRNAs as important components of tissue development and differentiation. Taken together, these results emphasize the immense potential of microarrays for sensitive and high-throughput analysis of miRNA expression in normal and disease states.
authors.affiliation: Ambion, Inc., 2130 Woodward Street, Austin, TX 78744-1832, USA.
authors.forename: Jaclyn
authors.initials: J
authors.lastname: Shingara
authors.forename: Kerri
authors.initials: K
authors.lastname: Keiger
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Shelton
authors.forename: Walairat
authors.initials: W
authors.lastname: Laosinchai-Wolf
authors.forename: Patricia
authors.initials: P
authors.lastname: Powers
authors.forename: Richard
authors.initials: R
authors.lastname: Conrad
authors.forename: David
authors.initials: D
authors.lastname: Brown
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Labourier
mesh_terms: D000328:Adult; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009928:Organ Specificity / Q000235:genetics
chemical_list: D035683:MicroRNAs