Molecular cloning and expression analysis of two hepcidin genes from olive flounder Paralichthys olivaceus.
Hepcidin is a cysteine-rich cationic antimicrobial peptide central to iron metabolism. We report a comparative analysis of the sequences, gene organization and expression of two hepcidin genes from olive flounder Paralichthys olivaceus. Both consist of two introns and three exons that encode a prepropeptide (81 amino acids for hepcidin I and 89 amino acids for hepcidin II). A TATA box and several consensus-binding motifs for transcription factors were found upstream of the transcriptional starting site. Hepcidin II was predominantly expressed in the liver and highly inducible under the effect of lipopolysaccharide (LPS), while a large amount of hepcidin I transcripts was detected in various tissues but did not appear to have a significant effect during LPS-stimulation.
Kim
Young-Ok
YO
Biotechnology Research Center, National Fisheries Research and Development Institute, 408-1 Gijang, Busan 619-0902, Korea. yokim@momaf.go.kr
Hong
Suhee
S
Nam
Bo-Hye
BH
Lee
Jeong-Ho
JH
Kim
Kyung-Kil
KK
Lee
Sang-Jun
SJ
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics*; D005432:Flounder / Q000235:genetics*; D020869:Gene Expression Profiling; D064451:Hepcidins; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D029941:Fish Proteins; D064451:Hepcidins; D020033:Protein Isoforms; D012313:RNA; D004247:DNA