The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses.
The completion of the 5,373,180-bp genome sequence of the marine psychrophilic bacterium Colwellia psychrerythraea 34H, a model for the study of life in permanently cold environments, reveals capabilities important to carbon and nutrient cycling, bioremediation, production of secondary metabolites, and cold-adapted enzymes. From a genomic perspective, cold adaptation is suggested in several broad categories involving changes to the cell membrane fluidity, uptake and synthesis of compounds conferring cryotolerance, and strategies to overcome temperature-dependent barriers to carbon uptake. Modeling of three-dimensional protein homology from bacteria representing a range of optimal growth temperatures suggests changes to proteome composition that may enhance enzyme effectiveness at low temperatures. Comparative genome analyses suggest that the psychrophilic lifestyle is most likely conferred not by a unique set of genes but by a collection of synergistic changes in overall genome content and amino acid composition.
Methé
Barbara A
BA
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. bmethe@tigr.org
Nelson
Karen E
KE
Deming
Jody W
JW
Momen
Bahram
B
Melamud
Eugene
E
Zhang
Xijun
X
Moult
John
J
Madupu
Ramana
R
Nelson
William C
WC
Dodson
Robert J
RJ
Brinkac
Lauren M
LM
Daugherty
Sean C
SC
Durkin
Anthony S
AS
DeBoy
Robert T
RT
Kolonay
James F
JF
Sullivan
Steven A
SA
Zhou
Liwei
L
Davidsen
Tanja M
TM
Wu
Martin
M
Huston
Adrienne L
AL
Lewis
Matthew
M
Weaver
Bruce
B
Weidman
Janice F
JF
Khouri
Hoda
H
Utterback
Terry R
TR
Feldblyum
Tamara V
TV
Fraser
Claire M
CM
D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D002244:Carbon / Q000378:metabolism; D003081:Cold Climate; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004734:Energy Metabolism; D020563:Gammaproteobacteria / Q000235:genetics* / Q000378:metabolism*; D016680:Genome, Bacterial; D023281:Genomics; D008386:Marine Biology; D008560:Membrane Fluidity; D008954:Models, Biological; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D040901:Proteomics; D013045:Species Specificity
D000596:Amino Acids; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D002244:Carbon; D009584:Nitrogen