Determination of cell tropism of HIV-1.
With the discovery that changes in the biological properties of HIV-1 correlate with the progression to disease, it became more and more important to develop assays to distinguish between the viral phenotypes. In this chapter, it is described how the biological phenotype of HIV-1 with regard to cellular tropism can be determined on primary monocyte-derived macrophages, established T-cell lines: MT2, SupT1, and H9, and promonocytic cell lines: U937, HL-60, and THP-1.
Kootstra
Neeltje A
NA
Department of Clinical Viro-Immunology Sanquin Research at CLB, Academical Medical Center, Amsterdam, The Netherlands.
Schuitemaker
Hanneke
H
D002461:Cell Line, Transformed; D002478:Cells, Cultured; D015497:HIV-1 / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D008264:Macrophages / Q000502:physiology / Q000821:virology*; D009000:Monocytes / Q000502:physiology / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013601:T-Lymphocytes / Q000502:physiology / Q000821:virology; D018523:Tropism / Q000502:physiology*; D020298:U937 Cells