Unexpected elevated production of Aquifex aeolicus cytochrome c555 in Escherichia coli cells lacking disulfide oxidoreductases.
Mutant strains of Escherichia coli lacking DsbA, DsbB, or DsbD (proteins required for disulfide bond formation in the periplasm) did not produce mitochondrial or chloroplast cytochromes c, as previously observed for bacterial ones. Unexpectedly, however, cytochrome c(555) (AA c(555)) from a hyperthermophile, Aquifex aeolicus, was produced in the E. coli periplasm without Dsb proteins, three times more than with them. These results indicate that the Dsb proteins are not necessarily required for AA c(555) production in E. coli, possibly because of hyperthermophilic origin compared with the others.
Kojima
Nobusuke
N
Graduate School of Biosphere Science, Hiroshima University, CREST of Japan Science and Technology Corporation.
Yamanaka
Masaru
M
Ichiki
Shin-ichi
S
Sambongi
Yoshihiro
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003574:Cytochrome c Group / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D019704:Protein Disulfide-Isomerases / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D008565:Membrane Proteins; D011994:Recombinant Proteins; C018887:cytochrome c-555; D010088:Oxidoreductases; D019704:Protein Disulfide-Isomerases