Theory of peak capacity in gradient elution.
Peak capacity is the best measure of the performance of a gradient separation. In this paper, the theory of peak capacity for the standard operating conditions of reversed-phase and ion-exchange chromatography is outlined. The influence of the operating conditions on the peak capacity of a separation are discussed. Finally, bandspreading phenomena in gradient chromatography are analyzed.
Neue
Uwe D
UD
Waters Corporation, 34 Maple Street, Milford, MA 01757-3696, USA. Uwe_Neue@Waters.com
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002852:Chromatography, Ion Exchange / Q000379:methods*; D008956:Models, Chemical; D011506:Proteins / Q000032:analysis / Q000737:chemistry*
D011506:Proteins