Deletion analysis of the sapovirus VP1 gene for the assembly of virus-like particles.
Human sapovirus (SaV) strains are agents of gastroenteritis. They cannot be grown in cell culture. In this study, constructs containing SaV N- and C-terminal-deleted recombinant capsid proteins (rVP1) were expressed in a baculovirus expression system to allow us to better understand the sequence requirements for the formation of virus-like particles (VLPs). Only proteins derived from N-terminal-deleted rVP1 constructs that began 49 nucleotides downstream assembled into VLPs, which included both small and native-size VLPs. Our results were similar to those reported in a rabbit hemorrhagic disease virus (RHDV) N- and C-terminal-deleted rVP1 expression study but were distinct from those reported in a norovirus N- and C-terminal-deleted rVP1 expression study, suggesting that SaV and RHDV may have similar expression requirements.
Hansman
G S
GS
Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan. ghansman@nih.go.jp
Matsubara
N
N
Oka
T
T
Ogawa
S
S
Natori
K
K
Takeda
N
N
Katayama
K
K
D016367:Baculoviridae; D036022:Capsid Proteins / Q000235:genetics / Q000502:physiology*; D005822:Genetic Vectors; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D029322:Norovirus / Q000235:genetics; D029341:Sapovirus / Q000235:genetics / Q000502:physiology*; D017384:Sequence Deletion; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D014772:Viroids / Q000648:ultrastructure; D019065:Virus Assembly / Q000235:genetics / Q000502:physiology*
D036022:Capsid Proteins; D014764:Viral Proteins