Genomewide cDNA microarray screening of genes related to benefits and toxicities of platinum-based chemotherapy in patients with advanced lung cancer.
The authors conducted a study using cDNA microarray analysis to determine whether expression levels of genes in tumors were correlated with the outcome of chemotherapy. Forty-seven patients were studied, and all except 3 received platinum-based chemotherapy. The expression levels of 1176 genes in transbronchial biopsy specimens of tumors that were obtained before chemotherapy were analyzed using the Atlas Human Cancer 1.2 Array. Multivariate regression analysis revealed that 3 genes were each independent factors related to tumor resistance to chemotherapy and patient survival (P < 0.01). Among various chemotherapy-related toxicities, 1, 3, 3, 1, and 1 genes were also revealed to be independent factors that were correlated with neutropenia, anemia, diarrhea, infection, and increased serum creatinine respectively (P < 0.01). It is concluded that not only the benefits but also the toxicities of chemotherapy can be predicted by cDNA microarray using tumor specimens obtained before chemotherapy.
Oshita
Fumihiro
F
Department of Thoracic Oncology, Kanagawa Cancer Center, Yokohama, Japan. foshita@kcch.jp
Ikehara
Mizuki
M
Sekiyama
Akiko
A
Hamanaka
Nobuyuki
N
Saito
Haruhiro
H
Yamada
Kouzo
K
Noda
Kazumasa
K
Kameda
Yoichi
Y
Miyagi
Yohei
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000740:Anemia / Q000097:blood / Q000139:chemically induced / Q000235:genetics; D000970:Antineoplastic Agents / Q000009:adverse effects* / Q000627:therapeutic use*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000235:genetics; D018288:Carcinoma, Small Cell / Q000188:drug therapy / Q000235:genetics; D003404:Creatinine / Q000097:blood; D003967:Diarrhea / Q000097:blood / Q000139:chemically induced / Q000235:genetics; D005260:Female; D015870:Gene Expression; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D007239:Infections / Q000097:blood / Q000139:chemically induced / Q000235:genetics; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009503:Neutropenia / Q000097:blood / Q000139:chemically induced / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D017671:Platinum Compounds / Q000009:adverse effects* / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D017671:Platinum Compounds; D003404:Creatinine