Time-course changes in the expression of heme oxygenase-1 in human subcutaneous hemorrhage.
To determine the time-course of human subcutaneous hemorrhage, heme oxygenase (HO)-1 expression and macrophage infiltration were observed using an immunohistochemical technique and semiquantitative analysis. The number of immunoreactive cells and the number of all infiltrating cells of each microscopic field were counted, and the ratio of the former to the latter was calculated as the positive cells ratio. An increase in the HO-1-positive cells ratio was observed starting at 3 h after injury, and the maximum ratio was observed 3 days after injury. The pattern of the increase in the macrophage ratio was similar to that of the HO-1-positive cells ratio in the early period after injury. Observation of serial sections revealed that the expression of HO-1 in the cells corresponded to the localization of macrophage. The present results suggest that the determination of HO-1 expression, as derived from macrophages, might be useful for the estimation of the time-course of subcutaneous hemorrhage.
Nakajima
Toru
T
Department of Legal Medicine, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan. keisei@ho.chiba-u.ac.jp
Hayakawa
Mutsumi
M
Yajima
Daisuke
D
Motani-Saitoh
Hisako
H
Sato
Yayoi
Y
Kiuchi
Masahiro
M
Ichinose
Masaharu
M
Iwase
Hirotaro
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D049429:Forensic Pathology; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006470:Hemorrhage / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism; D008875:Middle Aged; D011180:Postmortem Changes; D017695:Soft Tissue Injuries / Q000378:metabolism*; D013997:Time Factors
D051547:Heme Oxygenase-1