Transferrin receptor-1 and 2 expression in chronic lymphocytic leukemia.
Transferrin receptor (TfR)-1 and 2 mRNA and CD71 (TfR1) expression was analyzed in 118 CLL patients. Ninety-five out of 109 analyzed cases expressed CD71, mostly at a high level; 60% of CD71 (+) cases were IGH-mutated. All samples were TfR1 mRNA (+); TfR2-alpha/beta mRNA was detected in, respectively, 52/102 and 100/109 cases. Competitive RT-PCR showed widely divergent levels of TfR1 mRNA in cases with high CD71 expression, alluding to post-transcriptional control of TfR1 expression in CLL. The almost uniformly high CD71 expression in CLL is in keeping with the activated status of neoplastic cells, regardless of IGH mutational load.
Smilevska
Tatjana
T
Laboratory of Molecular Genetics and Cytogenetics, University of Thessaly, Larissa, Greece.
Stamatopoulos
Kostas
K
Samara
Maria
M
Belessi
Chrysoula
C
Tsompanakou
Aliki
A
Paterakis
George
G
Stavroyianni
Niki
N
Athanasiadou
Ioanna
I
Chiotoglou
Ioanna
I
Hadzidimitriou
Anastasia
A
Athanasiadou
Anastasia
A
Douka
Vassiliki
V
Saloum
Riad
R
Laoutaris
Nikolaos
N
Anagnostopoulos
Achilles
A
Fassas
Athanasios
A
Stathakis
Nikolaos
N
Kollia
Panagoula
P
D051997:ADP-ribosyl Cyclase 1 / Q000032:analysis; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis / Q000235:genetics*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D016130:Immunophenotyping; D037181:Lectins, C-Type; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D012333:RNA, Messenger / Q000032:analysis; D011990:Receptors, Transferrin / Q000032:analysis / Q000235:genetics*; D000099192:CD69 Antigens
D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D007143:Immunoglobulin Heavy Chains; D037181:Lectins, C-Type; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D000099192:CD69 Antigens; C068126:CD71 antigen; C503640:TFR2 protein, human