[Mycophenolic acid induced apoptosis in T lymphocytic cells Molt-4 and its mechanism].
To determine whether mycophenolic acid (MPA) exerts an apoptotic effect on human leukemic T cells Molt-4 and to elucidate its possible mechanism.



Cell morphology, DNA fragmentation, cell cycle,percentage of annexin V positive cells and enzymatic activity of caspase-3 were measured by microscopic, electrophoretic and flow cytometric techniques respectively. Human leukemic B cell line Raji was used as control.



MPA could induce apoptosis of Molt-4 cells with dose-and time-dependent manners; cells were blocked in the S phase. The activity of caspase-3 was enhanced in a dose-dependent manner in Molt-4 cells treated with MPA for 24 h. MPA could not induce apoptosis in Raji cells.



MPA can induce apoptosis in Molt-4 cells which may be involved in the activation of caspase-3.
Zhu
Xiao-li
XL
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Li
Li
L
Shi
Ji-min
JM
Zheng
Wei-yan
WY
Yu
Jian
J
Huang
He
H
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015458:Leukemia, T-Cell / Q000473:pathology*; D009173:Mycophenolic Acid / Q000494:pharmacology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D009173:Mycophenolic Acid