title: [Comparison of PCR and isoenzyme analysis in identification of Anopheles minimus A and C].
abstract: OBJECTIVE
  To compare the PCR and isoenzyme methods in identification of Anopheles minimus A and C.
  
  
  METHODS
  Samples of An. minimus collected in the field were identified from An. aconitus and An. jeyporiensis by PCR-RFLP after morphological examination, then classified by PCR-ASA. The mosquitoes identified by this method were further analyzed for isoenzyme to observe the difference, which was previously used as a technique to classify An. minimus A and C.
  
  
  RESULTS
  PCR method can differentiate An. minimus A and C simply and quickly. For Isoenzyme method, only EST allozyme pattern displayed difference.
  
  
  CONCLUSIONS
  PCR method is better than isoenzyme analysis in differentiating An. minimus A and C.
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis, Shanghai 200025, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zheng
authors.forename: Lin-Hua
authors.initials: LH
authors.lastname: Tang
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Ma
authors.forename: Xue-Zhong
authors.initials: XZ
authors.lastname: Wang
authors.forename: Shui-Sen
authors.initials: SS
authors.lastname: Zhou
authors.forename: Wen-Qi
authors.initials: WQ
authors.lastname: Shi
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D007527:Isoenzymes / Q000032:analysis; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D007527:Isoenzymes