Rv2358 and FurB: two transcriptional regulators from Mycobacterium tuberculosis which respond to zinc.
In a previous work, we demonstrated that the Mycobacterium tuberculosis Rv2358-furB operon is induced by zinc. In this study, the orthologous genes from Mycobacterium smegmatis mc(2)155 were inactivated and mutants analyzed. Rv2358 protein was purified and found to bind upstream of the Rv2358 gene. Binding was inhibited by Zn(2+) ions.
Canneva
Fabio
F
Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, via Ferrata 1, 27100 Pavia, Italy.
Branzoni
Manuela
M
Riccardi
Giovanna
G
Provvedi
Roberta
R
Milano
Anna
A
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D017353:Gene Deletion; D008969:Molecular Sequence Data; D020102:Mycobacterium smegmatis / Q000235:genetics* / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism; D009876:Operon / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000502:physiology; D011485:Protein Binding / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
D001426:Bacterial Proteins; D014157:Transcription Factors; D015032:Zinc