title: The effects of a novel synthetic retinoid, seletinoid G, on the expression of extracellular matrix proteins in aged human skin in vivo.
abstract: BACKGROUND
  Although retinoids have potential efficacy in aged skin, their side effect (skin irritation) remains a clinical problem. We designed a novel synthetic retinoid, seletinoid G, by using computer-aided molecular modeling, and investigated its effects on the expression of extracellular matrix proteins in human skin in vivo.
  
  
  METHODS
  Twenty-three subjects were tested on the buttocks using 4-day occlusive application of seletinoid G and all-trans retinoic acid (tRA). Skin irritation after topical application was quantified by the degree of erythema and cutaneous blood flow. The expression of extracellular matrix proteins and interstitial collagenase (MMP-1) in skin biopsies was investigated by immunohistochemical staining and Western blotting.
  
  
  RESULTS
  The topical application of seletinoid G under occlusion induced no skin irritation in contrast to tRA, which caused severe erythema. The topical treatment with seletinoid G increased the expressions of type I procollagen, tropoelastin, and fibrillin-1, and reduced MMP-1 in old skin in vivo. Seletinoid G was found to inhibit not only the UV-induced decrease of type I procollagen but the UV-induced increase of MMP-1 and c-Jun protein in young skin in vivo.
  
  
  CONCLUSIONS
  Seletinoid G is a novel synthetic retinoid, which has little the side effect of skin irritation after topical application. Seletinoid G can repair altered connective tissue in old skin and inhibit UV-induced collagen deficiency in young skin.
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Korea.
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Serah
authors.initials: S
authors.lastname: Lee
authors.forename: Ho Sik
authors.initials: HS
authors.lastname: Rho
authors.forename: Duck Hee
authors.initials: DH
authors.lastname: Kim
authors.forename: Ih Seop
authors.initials: IS
authors.lastname: Chang
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000328:Adult; D000368:Aged; D004148:Dioxolanes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D015394:Molecular Structure; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D011714:Pyrans / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D012176:Retinoids / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D004148:Dioxolanes; D016326:Extracellular Matrix Proteins; D016755:Proto-Oncogene Proteins c-jun; D011714:Pyrans; D012176:Retinoids; C507164:seletinoid G; D014212:Tretinoin; D020781:Matrix Metalloproteinase 1