Long-term kindled seizures induce alterations in hematopoietic functions: role of serum leptin.
Recent studies conducted in our laboratory have demonstrated marked increases in both serum leptin levels and colony numbers in bone marrow progenitor cells following long-term kindled seizures in rats. The present study sought to determine whether such changes in hematopoietic functions following kindling are linked to increased serum leptin levels. Kindled stage V seizures were induced for 30 days in Sprague-Dawley rats by stimulation of the basal complex of amygdala. The results revealed colony numbers in colony forming units-granulocyte/macrophage (CFU-GM) cultures from kindled rats increased significantly, an effect that was blocked by the presence of an anti-leptin antibody. The results further demonstrated that the addition of serum obtained from kindled rats to CFU-GM cultures from control rats significantly increased the numbers of colonies relative to non-serum added cultures. Moreover, the proliferative effects of serum from kindled rats were also blocked by adding an anti-leptin antibody. These findings were confirmed from the observations that the long isoform of the leptin receptor, which is capable of signal transduction, was present only in kindled, but not in control rats. Thus, the results provide evidence that the hematopoietic changes observed following long-term kindling are directly associated with elevated serum leptin levels.
Bhatt
Rekha
R
Department of Neurology and Neurosciences, MSB, H-512, University of Medicine and Dentistry, Newark, NJ 07103, USA.
Bhatt
Suresh
S
Rameshwar
Pranela
P
Siegel
Allan
A
D000704:Analysis of Variance; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002452:Cell Count; D003114:Colony-Forming Units Assay / Q000379:methods; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects / Q000502:physiology*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D007696:Kindling, Neurologic / Q000187:drug effects; D020738:Leptin / Q000097:blood* / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D054411:Receptors, Leptin; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012640:Seizures / Q000097:blood* / Q000503:physiopathology; D013997:Time Factors
D000906:Antibodies; D020738:Leptin; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D007041:Hypoxanthine Phosphoribosyltransferase