Mapping of the binding site for Mannheimia haemolytica leukotoxin within bovine CD18.
To map the site involved in Mannheimia haemolytica leukotoxin (LktA) binding and biological activity within bovine CD18, bovine x human CD18 chimeric constructs were generated and coexpressed with bovine CD11a in K562 cells. Studies with the chimeric leukocyte function-associated antigen 1 transductants demonstrate that the site required for LktA binding and biological effects resides within amino acid residues 500 and 600 of the extracellular region of bovine CD18.
Dileepan
T
T
Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA.
Kannan
M S
MS
Walcheck
B
B
Thumbikat
P
P
Maheswaran
S K
SK
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000276:immunology*; D015153:Blotting, Western; D040881:CD11a Antigen / Q000276:immunology; D018821:CD18 Antigens / Q000276:immunology*; D002417:Cattle; D005098:Exotoxins / Q000276:immunology*; D006460:Hemolysin Proteins / Q000276:immunology*; D016978:Mannheimia haemolytica / Q000276:immunology*; D016871:Pasteurellaceae Infections / Q000276:immunology
D001426:Bacterial Proteins; D040881:CD11a Antigen; D018821:CD18 Antigens; D005098:Exotoxins; D006460:Hemolysin Proteins; C102677:lktA protein, bacteria