Rabbit monoclonal antibodies: a comparative study between a novel category of immunoreagents and the corresponding mouse monoclonal antibodies.
Rabbit monoclonal antibodies (RabMAbs) represent a novel category of immunoreagents that may combine the best properties of both mouse monoclonal antibodies (MMAs) and of rabbit antisera. In the attempt to verify the performance of this new class of antibodies on paraffin-embedded tissue, RabMAbs against estrogen receptor, progesterone receptor, Ki-67, cyclin D1, CD3, CD5, CD23, and synaptophysin were tested on several tumor types as well as normal tissues. The results were compared with those obtained with classic MMAs against the same antigens. RabMAbs appear to offer increased sensitivity with no apparent loss of specificity. On routine use they permit higher working dilutions (5 to 10 times on average), allowing significant improvement in terms of laboratory efficiency. The robustness of RabMAbs is further proved by the fact that in some instances optimal staining can be obtained even without antigen retrieval. In consideration of the high performance observed, routine use of RabMAbs may contribute significantly to standardize diagnostic immunohistochemical procedures.
Rossi
Sabrina
S
Department of Pathology, Regional Hospital, Treviso, Italy.
Laurino
Licia
L
Furlanetto
Alberto
A
Chinellato
Serena
S
Orvieto
Enrico
E
Canal
Fabio
F
Facchetti
Fabio
F
Dei Tos
Angelo P
AP
D000818:Animals; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis*; D017252:CD3 Complex / Q000378:metabolism; D018956:CD5 Antigens / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods*; D019394:Ki-67 Antigen / Q000378:metabolism; D051379:Mice; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D011817:Rabbits; D011960:Receptors, Estrogen / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D012680:Sensitivity and Specificity; D016708:Synaptophysin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D017252:CD3 Complex; D018956:CD5 Antigens; D019394:Ki-67 Antigen; D011960:Receptors, Estrogen; D017455:Receptors, IgE; D011980:Receptors, Progesterone; D016708:Synaptophysin; D019938:Cyclin D1