[Expression of two plant agglutinin genes in transgenic tobacco plants].
A plant expression vector pBACG containing the DNA sequence coding for Amaranthus caudatus agglutinin (ACA) and a modified Glanthus nivalis agglutinin (GNA) gene was constructed. Leaf explants of Nicotiana tobacum cv. SRI were transformed with A. tumefaciens LBA4404 harbouring the above expression vector. Results from PCR and Southern blotting analysis showed that both the ACA and GNA gene were inserted into the genome of transformed tobacco plants. Western blottingting analysis of soluble protein isolated from transgenic plants showed that ACA and GNA were synthesized. The results from insect bioassay with peach aphids ( Myzus persicae) revealed that the transgenic plants of pBACG had acquired high resistance against peach aphids. The average aphid-inhibition rate reached up to 83.9% and 85.3% for transgenic plants (T0) and their selfed progenies (T1) respectively,indicating that the functions of these two genes were inheritable.
Liu
Zhao-Hua
ZH
Institution of Microbiology, Chinese Academy of Sciences, National Key Laboratory of Plant Genomics, Beijing 100080, China. liuzhaohua3333@sohu.com
Zhang
Zhen-Shan
ZS
Guo
Hong-Nian
HN
Jia
Yan-Tao
YT
Zheng
Guang-Yu
GY
Tian
Ying-Chuan
YC
D016960:Agrobacterium tumefaciens / Q000235:genetics; D027721:Amaranthus / Q000235:genetics; D000818:Animals; D001042:Aphids / Q000254:growth & development; D015139:Blotting, Southern; D015153:Blotting, Western; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D007113:Immunity, Innate / Q000235:genetics; D037241:Mannose-Binding Lectins / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D037121:Plant Lectins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology; D014170:Transformation, Genetic
D018744:DNA, Plant; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; C063117:snowdrop lectin