Prevention of neomycin-induced nephrotoxic event in pig proximal tubular epithelial cell line by apolipoprotein E3.
Nephrotoxicity is one of critical problems of aminoglycoside antibiotics. We examined the protective effect of apolipoprotein E3 (apoE3), one of ligands for megalin, on neomycin-induced extracellular release of lactate dehydrogenase, a marker of cell necrosis using pig proximal tubular LLC-PK1 cells. Neomycin significantly induced the extracellular release of lactate dehydrogenase, but apoE3 successfully suppressed it. This result indicated that apoE3 protects the proximal tubular cells from the eventual cell death induced by nephrotoxic aminoglycosides.
Takamoto
Kozo
K
Numazu Bio-Medical Research Institute, Microbial Chemistry Research Center, 18-24 Miyamoto, Numazu-shi, Shizuoka 410-0301, Japan.
Kawada
Manabu
M
Ikeda
Daishiro
D
D000818:Animals; D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D053318:Apolipoprotein E3; D001057:Apolipoproteins E / Q000494:pharmacology*; D002460:Cell Line; D004847:Epithelial Cells / Q000201:enzymology / Q000473:pathology*; D005110:Extracellular Space / Q000201:enzymology / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced* / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000201:enzymology / Q000473:pathology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018374:LLC-PK1 Cells; D009355:Neomycin / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D013552:Swine
D000900:Anti-Bacterial Agents; D053318:Apolipoprotein E3; D001057:Apolipoproteins E; D007770:L-Lactate Dehydrogenase; D009355:Neomycin