Simple fluorescent pyrimidine analogues detect the presence of DNA abasic sites.
A family of simple pyrimidine analogues has been synthesized, and their photophysical properties have been investigated. The most responsive of the family was incorporated in DNA. This isosteric fluorescent DNA analogue monitors denaturation of a DNA duplex via fluorescence and positively detects the presence of abasic sites in DNA duplexes.
Greco
Nicholas J
NJ
Department of Chemistry and Biochemistry, University of California-San Diego, La Jolla, CA 92093, USA.
Tor
Yitzhak
Y
D004247:DNA / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D011743:Pyrimidines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D011743:Pyrimidines; D004247:DNA; C030986:pyrimidine