Soluble angiopoietin receptor Tie-2 in patients with acute myocardial infarction and its effects on angiogenesis.
Tie-2 receptor has been shown to play a role in the neovascularization of tumors, but little is known about the role it may play in acute myocardial infarction (AMI). The aims of this research are (1) To study the variety of soluble Tie-2 (sTie-2) in patients with AMI. (2) To study the effects of recombinant soluble Tie-2/Fc on HUVECs viability and tube formation in vitro. Serum levels of sTie-2 in 27 patients with AMI were measured on admission (day 1), day 2, day 3 and day 7 after onset of chest pain and 28 healthy controls by ELISA. In addition, the viability of HUVECs and tube formation area were measured after stimulated with recombinant Tie-2/Fc chimera. Median level of sTie-2 increased significantly in the AMI patients when compared with the controls and the maximum level appeared at day 2 after onset of AMI. Tie-2/Fc induced EC apoptosis and inhibited HUVEC tube formation in vitro. Results of this study showed that the level of sTie-2 increased in AMI. The effects of Tie-2/Fc on EC viability and tube formation indicated that angiogenesis might be inhibited in the acute phase of AMI.
Wang
Chunling
C
Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, FuWai Cardiovascular Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China.
Fu
Panfeng
P
Li
Hongwei
H
Gao
Runlin
R
Xiu
Ruijuan
R
D000368:Aged; D016022:Case-Control Studies; D002470:Cell Survival / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000473:pathology*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D042787:Receptor, TIE-2 / Q000097:blood* / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology*; D012995:Solubility; D013997:Time Factors; D014471:Umbilical Veins
D007141:Immunoglobulin Fc Fragments; D011994:Recombinant Proteins; D042787:Receptor, TIE-2