title: Identification and characterization of a canine highly similar to retinoic acid receptor alpha.
abstract: A canine highly similar to retinoic acid receptor alpha (canine HS-RARa) cDNA was isolated from the spleen tissue. A database search and the alignment revealed that the canine cDNA was most similar to highly similar type of human RARa and was named canine HS-RARa. The expression of the genes encoding RARa in the dog was the highest in the testis and moderate in the blood, lymph node, mammary gland, pancreas, salivary gland, spleen, thyroid gland, tonsil and uterus. The nucleotide sequence encoded the 462-amino acid containing the conserved sequence motif of RARa. Though the amino acid sequences were well-conserved among species, some unique arrangements were observed within each class. In the phylogenetic analysis, each species separated according to their class. In the branch of mammals, the dog is in the cluster of humans, mice and western wild mice. However, hamsters and rats formed another branch.
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Miyajima
authors.forename: Manabu
authors.initials: M
authors.lastname: Watanabe
authors.forename: Emi
authors.initials: E
authors.lastname: Ohashi
authors.forename: Keitaro
authors.initials: K
authors.lastname: Ohmori
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nishimura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D051381:Rats; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D000072482:Retinoic Acid Receptor alpha; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; C000607071:RARA protein, human; C000607072:Rara protein, mouse; C000607073:Rara protein, rat; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha