title: Sensitive determination of alpha-methyltryptamine (AMT) and 5-methoxy-N,N-diisopropyltryptamine (5MeO-DIPT) in whole blood and urine using gas chromatography-mass spectrometry.
abstract: We devised a sensitive and simple method to determine alpha-methyltryptamine (AMT) and 5-methoxy-N,N-diisopropyltryptamine (5MeO-DIPT) in whole blood and urine, using gas chromatography-mass spectrometry (GC-MS). AMT and 5MeO-DIPT were extracted using an Extrelut column with an internal standard, bupivacaine, followed by derivatization with acetic anhydride. The derivatized extract was used for GC-MS analysis of EI-SIM mode. The calibration curves of AMT and 5MeO-DIPT were linear in the concentration range from 10 to 750 ng/ml in both blood and urine samples. The method detection limit (MDL) of AMT and 5MeO-DIPT were 1 ng/ml each in whole blood and 5 ng/ml each in urine. This method should be most useful to accurately determine the presence of these drugs in blood and urine in clinical and forensic cases.
authors.affiliation: Department of Forensic Pathology and Sciences, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ishida
authors.forename: Keiko
authors.initials: K
authors.lastname: Kudo
authors.forename: Akiko
authors.initials: A
authors.lastname: Kiyoshima
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Inoue
authors.forename: Akiko
authors.initials: A
authors.lastname: Tsuji
authors.forename: Noriaki
authors.initials: N
authors.lastname: Ikeda
mesh_terms: D008735:5-Methoxytryptamine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D014363:Tryptamines / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D014363:Tryptamines; C033696:5-methoxy-N,N-diisopropyltryptamine; D008735:5-Methoxytryptamine; C015293:indopan