Enhancement of inosine production by Bacillus subtilis through suppression of carbon overflow by sodium citrate.
Sodium citrate, added to culture medium at 2 g l-1, increased inosine production by B. subtilis by 18% to 16 g l-1. The phosphoenolpyruvate (PEP) pool was increased by 2.3-fold, while the pyruvate and acetate pools were decreased by 78% and 57%, respectively. Pyruvate kinase might thus be a regulatory site for inosine synthesis.
Chen
Shuangxi
S
State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, P.O. Box 329, 200237, Shanghai , P.R. China.
Chu
Ju
J
Zhuang
Yingping
Y
Zhang
Siliang
S
D000085:Acetates / Q000378:metabolism; D001412:Bacillus subtilis / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002950:Citrate (si)-Synthase / Q000378:metabolism; D002951:Citrates / Q000494:pharmacology*; D005941:Glucokinase / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007288:Inosine / Q000096:biosynthesis*; D010728:Phosphoenolpyruvate / Q000378:metabolism; D025221:Phosphofructokinases / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D000077559:Sodium Citrate; D013997:Time Factors
D000085:Acetates; D002264:Carboxylic Acids; D002951:Citrates; D000077559:Sodium Citrate; D007288:Inosine; D010728:Phosphoenolpyruvate; D019289:Pyruvic Acid; D005954:Glucosephosphate Dehydrogenase; D002950:Citrate (si)-Synthase; D025221:Phosphofructokinases; D005941:Glucokinase; D011770:Pyruvate Kinase; D005947:Glucose