Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro.
We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS-2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (approximately 07:00 to 23:00 h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time-PCR analysis. DEX stimulation of human BEAS-2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes.
Burioka
Naoto
N
Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, Japan. burioka@grape.med.tottori-u.ac.jp
Takata
Miyako
M
Okano
Yoko
Y
Ohdo
Shigehiro
S
Fukuoka
Yasushi
Y
Miyata
Masanori
M
Takane
Hiroshi
H
Endo
Masahiro
M
Suyama
Hisashi
H
Shimizu
Eiji
E
D000328:Adult; D001980:Bronchi / Q000033:anatomy & histology; D018797:Cell Cycle Proteins; D002460:Cell Line; D002940:Circadian Rhythm / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D056950:Period Circadian Proteins; D020545:Respiratory Mucosa / Q000187:drug effects / Q000502:physiology
D018797:Cell Cycle Proteins; D005938:Glucocorticoids; D009687:Nuclear Proteins; C539022:PER1 protein, human; D056950:Period Circadian Proteins; D003907:Dexamethasone