title: Developmental validation of the quantifiler real-time PCR kits for the quantification of human nuclear DNA samples.
abstract: The Quantifiler Human DNA Quantification Kit and the Quantifiler Y Human Male DNA Quantification Kit were designed for the quantification of human genomic DNA in forensic samples. The kits use a real-time PCR-based process to quantify, respectively, total human DNA or human male DNA only. We report the results of a developmental validation study that we performed with the Quantifiler Kits, following the official SWGDAM guidelines. The Quantifiler Kits were tested for performance criteria such as species specificity, sensitivity, stability, precision and accuracy, and in addition, were tested with forensic case-type samples and mixed (male:female) DNA samples. The Quantifiler Kit methods were highly specific for human DNA, and could detect as little as 32 picograms of DNA using 2 microL of sample per assay. The accuracy and precision of the Quantifiler Kit methods was comparable or superior to that of other quantification methods.
authors.affiliation: Applied Biosystems, Foster City, CA 94404, USA. greenrx@appliedbiosystems.com
authors.forename: Robert L
authors.initials: RL
authors.lastname: Green
authors.forename: Ines C
authors.initials: IC
authors.lastname: Roinestad
authors.forename: Cherisse
authors.initials: C
authors.lastname: Boland
authors.forename: Lori K
authors.initials: LK
authors.lastname: Hennessy
mesh_terms: D000818:Animals; D041322:Chromosomes, Human, Y; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA