Quantification of phosphocholine and glycerophosphocholine with 31P edited 1H NMR spectroscopy.
Choline and the related compounds phosphocholine (PC) and glycerophosphocholine (GPC) are considered to be important metabolites in oncology. Past studies have demonstrated correlations linking the relative ratios and concentrations of these metabolites with the development and progression of cancer. Currently, in vivo and tissue ex vivo magnetic resonance spectroscopy methods have mostly centered on measuring the total concentration of these metabolites and have difficulty in differentiating between them. Here, a new scheme that uses (31)P edited (1)H spectroscopy to quantify the concentrations of choline, PC and GPC in biological samples is reported and its applicability is demonstrated using samples of human brain tumor extracts. This method is particularly well-suited for analytical situations where the PC and GPC resonances are not sufficiently resolved and/or are obscured by other metabolites. Consequently, this scheme has the potential to be used for the analysis of choline compounds in ex vivo tissue samples.
Loening
Nikolaus M
NM
Department of Chemistry, Lewis & Clark College, Portland, OR 97219, USA. loening@lclark.edu
Chamberlin
Anne M
AM
Zepeda
Andrea G
AG
Gonzalez
R Gilberto
RG
Cheng
Leo L
LL
D014408:Biomarkers, Tumor / Q000737:chemistry / Q000378:metabolism; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000737:chemistry / Q000378:metabolism; D005997:Glycerylphosphorylcholine / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010759:Phosphorus Isotopes / Q000737:chemistry*; D010767:Phosphorylcholine / Q000737:chemistry* / Q000378:metabolism; D012015:Reference Standards; D014020:Tissue Extracts / Q000737:chemistry
D014408:Biomarkers, Tumor; D010759:Phosphorus Isotopes; D014020:Tissue Extracts; D010767:Phosphorylcholine; D005997:Glycerylphosphorylcholine