title: [Study on human leukocyte antigen G1 reducing xeno-cell-rejection by transfecting porcine endothelial cells].
abstract: OBJECTIVE
  To study whether the porcine endothelial cells (PECs) lines transfected by HLA-G1 can alter the lysis mediated by human peripheral blood mononuclear cell (PBMC) and natural killer cell 92 (NK-92).
  
  
  METHODS
  By use of liposomes pack, the pcDNA3. 0 eukaryotic expression vector carrying HLA-G1 was transfected into PECs. Using indirect immunofluorescence and RT-PCR assays, the HLA-G1 expression in PECs was detected. The alteration of the lysis mediated by PBMC and NK-92 was detected by 51Cr-release assays.
  
  
  RESULTS
  HLA-G1 expression could be detected in PECs after transfection of HLA-G1 at the levels of protein and RNA. It also could be found that the survival rate of transfected PECs was much higher than that of non-transfected PECs, when both of them faced the lysis mediated by human PBMC and NK-92. After transfecting the expression of HLA-G1 could be found in the transfected PECs and the lysis mediated by PBMC and NK-92 to PECs decreased obviously (P<0.05).
  
  
  CONCLUSIONS
  The PECs transfected by HLA-G1 can decrease the NK lysis, so that it may provide us a new thought to inhibit the xeno-cell-rejection.
authors.affiliation: Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, P. R. China.
authors.forename: Zhenxiong
authors.initials: Z
authors.lastname: Xia
authors.forename: Junyan
authors.initials: J
authors.lastname: Han
authors.forename: Shusen
authors.initials: S
authors.lastname: Wang
authors.forename: Ronghua
authors.initials: R
authors.lastname: Zeng
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Honggang
authors.initials: H
authors.lastname: Qi
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Xiongwen
authors.initials: X
authors.lastname: Wu
authors.forename: Feili
authors.initials: F
authors.lastname: Gong
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D042783:Endothelial Cells / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation*; D019084:Fluorescent Antibody Technique, Indirect; D005822:Genetic Vectors; D006084:Graft Rejection / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014162:Transfection / Q000379:methods*; D014183:Transplantation, Heterologous
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I