[Effects of CD147 on the production of matrix metalloproteinase-9 by fibroblasts and the invasion of melanoma cells].
OBJECTIVE
To investigate a possible role of CD147 in the production of matrix metalloproteinase-9 (MMP-9) by fibroblasts and the invasion of melanoma cells.


METHODS
We cocultured CD147-expression melanoma cells with fibroblasts and examined the MMP-9 expression of fibroblasts by zymography and the invasion of melanoma cells by transwell invasion assay.


RESULTS
MMP-9 expression was enhanced in conditioned media, while cocultured with melanoma cells in a dose-dependent manner, and CD147 antibody inhibited the production of MMP-9 in the fibroblasts. When fibroblasts were cultured at the bottom of the lower compartment of transwell invasion model, the number of melanoma cells that invaded significantly increased. Addition of anti-CD147 antibody to the upper compartment transwell invasion model resulted in the significant inhibition of the melanoma cell invasion in reconstituted basement membrane.


CONCLUSIONS
CD147 expressed in melanoma cells plays an important role in the melanoma cell invasion by stimulating the production of MMP-9 by fibroblasts.
Chen
Xiang
X
Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008. China. chenxck@yahoo.com
Tian
Li-Hong
LH
Xie
Hong-Fu
HF
Shi
Wei
W
Feng
Hao
H
Li
Ji
J
Chen
Fu-Wen
FW
D051926:Basigin / Q000276:immunology / Q000494:pharmacology*; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D008545:Melanoma / Q000473:pathology*; D009361:Neoplasm Invasiveness; D014407:Tumor Cells, Cultured
D051926:Basigin; D020780:Matrix Metalloproteinase 9