Dogs cloned from adult somatic cells.
Several mammals--including sheep, mice, cows, goats, pigs, rabbits, cats, a mule, a horse and a litter of three rats--have been cloned by transfer of a nucleus from a somatic cell into an egg cell (oocyte) that has had its nucleus removed. This technology has not so far been successful in dogs because of the difficulty of maturing canine oocytes in vitro. Here we describe the cloning of two Afghan hounds by nuclear transfer from adult skin cells into oocytes that had matured in vivo. Together with detailed sequence information generated by the canine-genome project, the ability to clone dogs by somatic-cell nuclear transfer should help to determine genetic and environmental contributions to the diverse biological and behavioural traits associated with the many different canine breeds.
Lee
Byeong Chun
BC
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Gwanak-gu, Seoul 151-742, South Korea.
Kim
Min Kyu
MK
Jang
Goo
G
Oh
Hyun Ju
HJ
Yuda
Fibrianto
F
Kim
Hye Jin
HJ
Hossein
M Shamim
MS
Shamim
M Hossein
MH
Kim
Jung Ju
JJ
Kang
Sung Keun
SK
Schatten
Gerald
G
Hwang
Woo Suk
WS
D000375:Aging; D000818:Animals; D019976:Cloning, Organism; D004285:Dogs / Q000145:classification / Q000196:embryology / Q000235:genetics* / Q000254:growth & development*; D004624:Embryo Transfer; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D053652:Nuclear Transfer Techniques; D018587:Oocyte Donation; D009865:Oocytes / Q000166:cytology / Q000502:physiology / Q000637:transplantation; D011247:Pregnancy; D012867:Skin / Q000166:cytology; D013997:Time Factors