title: In vitro Epstein-Barr virus-immortalized lymphoma cell line carrying t(9;14)(p13;q32) chromosome abnormality, derived from splenic lymphoma with villous lymphocytes.
abstract: We herein describe splenic lymphoma with villous lymphocytes (SLVL) carrying t(9;14)(p13;q32). The t(9;14)(p13;q32) is a rare reciprocal chromosome translocation found in a subset of B-cell malignancies, mainly in low-grade non-Hodgkin's lymphomas. In t(9;14)(p13;q32), PAX-5 gene on 9p13 is involved with the immunoglobulin heavy-chain gene on 14q32. It has been thought that the deregulated expression of PAX-5 as a result of t(9;14)(p13;q32) may contribute to abnormal cell proliferation. Although continuous cell lines are invaluable tools for studying lymphomagenesis in the t(9;14)(p13;q32)-bearing lymphomas, establishment of such cell lines is extremely difficult since they are usually mature B-cell malignancies. In an attempt to transform the SLVL cells into a proliferating cell line, we examined the responses of the cells to infection by Epstein-Barr virus (EBV). SLVL cells were found to be susceptible to immortalization by EBV, resulting in a permanent cell line. The cell line, designated SL-15, possessed the t(9;14)(p13;q32). Genotype analysis and immunophenotype profiles confirmed that the cell line arose from the primary lymphoma cells. The cells had characteristic cytoplasmic villi. SL-15 cells has been growing over 2 years equivalent to 350-400 population doubling levels without proliferative crisis that is often observed in EBV-positive lymphoblastoid cell lines. Furthermore, SL-15 cells, when inoculated into nude mice, formed t(9;14)(p13;q32)-bearing tumors with cytoplasmic villi. The validated SLVL-derived cell line provide a useful model system to study molecular biology of t(9;14)(p13;q32)-bearing B-cell malignancies as well as lymphomagenesis of SLVL in vitro and in vivo.
authors.affiliation: Department of Hematology and Respiratory Medicine, Kochi Medical School, Kochi University, Kochi, Japan. daibatam@med.kochi-u.ac.jp
authors.forename: Masanori
authors.initials: M
authors.lastname: Daibata
authors.forename: Takahiro
authors.initials: T
authors.lastname: Taguchi
authors.forename: Yuiko
authors.initials: Y
authors.lastname: Nemoto
authors.forename: Shinji
authors.initials: S
authors.lastname: Iwasaki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: Hirokuni
authors.initials: H
authors.lastname: Taguchi
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002883:Chromosomes, Human, Pair 14; D002899:Chromosomes, Human, Pair 9; D020031:Epstein-Barr Virus Infections; D005260:Female; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000472:pathogenicity*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008214:Lymphocytes / Q000648:ultrastructure; D008223:Lymphoma / Q000235:genetics* / Q000821:virology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D051757:PAX5 Transcription Factor / Q000235:genetics / Q000502:physiology; D013160:Splenic Neoplasms / Q000235:genetics* / Q000821:virology*; D014178:Translocation, Genetic; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D007143:Immunoglobulin Heavy Chains; D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human