title: [Cyclosporin a induces titin expression in human trophoblast cells through the MEK/ERK1/2 signal pathway].
abstract: To investigate the role of MEK/ERK1/2 signal pathway in the regulation of cyclosporin A(CsA) -induced titin expression in human trophoblast cells. With RT-PCR and Western Blot, We examined the titin expression level of human trophoblast cells treated with different concentrations of CsA for various duration, then detected total ERK1/2 and phosphorated ERK1/2 level with Western Blot, and observed effect of U0126 on transcription of titin mRNA in human trophoblast cells stimulated by cyclosporin A. It was found that CsA could activate ERK1/2 in time-dependent and dosage-dependent manner, and induced titin to be expressed in human trophoblast cells. U0126, a MEK inhibitor, inhibited the transcription of titin in dosage-dependent manner. These results indicated that MEK/ERK1/2 signal pathway may play an important role in the expression of titin in human trophoblast induced by cyclosporin A.
authors.affiliation: Laboratory of Reproductive Immunology, Institute of Obstetrics and Gynecology, Fudan University, Shanghai 200011, China.
authors.forename: Mei Rong
authors.initials: MR
authors.lastname: Du
authors.forename: Da Jin
authors.initials: DJ
authors.lastname: Li
authors.forename: Yuan Chang
authors.initials: YC
authors.lastname: Yan
authors.forename: Li Ping
authors.initials: LP
authors.lastname: Jin
authors.forename: Ming Yan
authors.initials: MY
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Min Min
authors.initials: MM
authors.lastname: Yuan
authors.forename: Yi
authors.initials: Y
authors.lastname: Meng
mesh_terms: D015153:Blotting, Western; D002478:Cells, Cultured; D064211:Connectin; D016572:Cyclosporine / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D011247:Pregnancy; D011494:Protein Kinases / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D064211:Connectin; D004791:Enzyme Inhibitors; D009124:Muscle Proteins; C577285:TTN protein, human; D016572:Cyclosporine; D011494:Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020929:Mitogen-Activated Protein Kinase Kinases