title: High-performance liquid chromatography with post-column derivatisation and fluorescence detection for sensitive determination of aflatoxin M1 in milk and cheese.
abstract: A new HPLC method with fluorescence detection using pyridinium hydrobromide perbromide as a post-column derivatising agent has been developed to determine aflatoxin M1 in milk and cheese. The detection limits were 1 ng/kg for milk and 5 ng/kg for cheese. The calibration curve was linear from 0.001 to 0.1 ng injected. The method includes a preliminary C18-SPE clean-up and the average recoveries of Aflatoxin M1 from milk and cheese, spiked at levels of 25-75 ng/kg and 100-300 ng/kg, respectively, were 90 and 76%; the precision (RSDr) ranged from 1.7 to 2.6% for milk and from 3.5 to 6.5% for cheese. The method is rapid, easily automatable and therefore useful for accurate and precise screening of aflatoxin M1 in milk and cheese.
authors.affiliation: Department of Food and Feed Science, University of Teramo, Viale F. Crispi 212, 64100 Teramo, Italy. a_manetta@virgilio.it
authors.forename: Anna Chiara
authors.initials: AC
authors.lastname: Manetta
authors.forename: Lorella
authors.initials: L
authors.lastname: Di Giuseppe
authors.forename: Melania
authors.initials: M
authors.lastname: Giammarco
authors.forename: Isa
authors.initials: I
authors.lastname: Fusaro
authors.forename: Anselmo
authors.initials: A
authors.lastname: Simonella
authors.forename: Alessandro
authors.initials: A
authors.lastname: Gramenzi
authors.forename: Andrea
authors.initials: A
authors.lastname: Formigoni
mesh_terms: D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002611:Cheese / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D008892:Milk / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D016607:Aflatoxin M1