Real-time fluorescence polymerase chain reaction (PCR) identification of Streptococcus pneumoniae from pleural fluid and tissue.
A diagnosis of pneumococcal pneumonia and empyema was established by real-time fluorescence polymerase chain reaction (PCR) performed in pleural fluid and tissue samples from a child in whom cultures were negative. This case demonstrates that the use of solid-phase nucleic acid purification technology and real-time fluorescence PCR have an application in the rapid diagnosis of S. pneumoniae pneumonia.
Poulter
Melinda D
MD
Department of Pathology and Laboratory Medicine, UCLA Medical Center, USA.
Deville
Jaime G
JG
Cherry
James D
JD
Shapiro
Alan M
AM
McAvin
James C
JC
D015373:Bacterial Typing Techniques; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D016724:Empyema, Pleural / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D005453:Fluorescence; D006801:Humans; D010994:Pleura / Q000382:microbiology*; D010996:Pleural Effusion / Q000382:microbiology*; D011018:Pneumonia, Pneumococcal / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial