High-throughput analysis of vitamin C in human plasma with the use of HPLC with monolithic column and UV-detection.
Vitamin C plays a central role in the body. One of its important functions is its role as an antioxidant, and accurate measurements are important for interpretations of this role. However, its reactive nature and instability complicates the assessment, especially in biological samples. A high-throughput chromatographic method using monolithic column and UV-detection was developed for the assessment of plasma ascorbic acid and total ascorbic acid. The method showed excellent analytical sensitivity, specificity, precision, recovery and linearity during the validation study. The method was used for the assessment of ascorbic acid and total ascorbic acid during several clinical studies.
Karlsen
Anette
A
Institute of Basic Medical Sciences, Department of Nutrition Research, University of Oslo, P.O. Box 1046, Blindern, N-0316 Oslo, Norway.
Blomhoff
Rune
R
Gundersen
Thomas E
TE
D000328:Adult; D001205:Ascorbic Acid / Q000097:blood* / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003683:Dehydroascorbic Acid / Q000097:blood; D006801:Humans; D007223:Infant; D008875:Middle Aged; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid