title: Role of NQO1 polymorphisms as risk factors for squamous cell carcinoma of the head and neck.
abstract: A case: control study was carried out to determine if inactivating polymorphisms of the NQO1 gene at bases 609 and 465 are associated with altered risk of developing squamous cell carcinoma of the head and neck (SCCHN). Genotyping was carried out by PCR RFLP analysis on whole blood samples. The frequency of the inactive 609T and active 609C forms, and the inactive 465T and active 465C forms, of NQO1 were compared in patient and control groups by a logistic regression analysis and odds ratios (ORs) were calculated. Participants were stratified by tobacco and alcohol use, and genotype distributions in these sub-groups were compared. There were no significant differences in genotype distribution between SCCHN patients and the control population for the base 609 or 465 polymorphisms. There were also no significant differences in genotype distributions between patient and control groups for tobacco and/or alcohol users and non-users. Genotype distributions were similar for SCCHN patients at all disease sites with the exception of the nasopharynx where there was a higher incidence of the 609C:609T and 609T:609T genotypes. These results suggest that individuals having either 609T or 465T alleles generally do not have an altered risk of developing SCCHN.
authors.affiliation: Manitoba Institute of Cell Biology, CancerCare Manitoba, 675 McDermot Avenue, Winnipeg, Man., Canada. begleit@cc.umanitoba.ca
authors.forename: Asher
authors.initials: A
authors.lastname: Begleiter
authors.forename: Alia
authors.initials: A
authors.lastname: Norman
authors.forename: Darren
authors.initials: D
authors.lastname: Leitao
authors.forename: Teresa
authors.initials: T
authors.lastname: Cabral
authors.forename: Donna
authors.initials: D
authors.lastname: Hewitt
authors.forename: Sushu
authors.initials: S
authors.lastname: Pan
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Grandis
authors.forename: Jill M
authors.initials: JM
authors.lastname: Siegfried
authors.forename: Samy
authors.initials: S
authors.lastname: El-Sayed
authors.forename: Donna
authors.initials: D
authors.lastname: Sutherland
authors.forename: David A
authors.initials: DA
authors.lastname: Ross
authors.forename: Paul D
authors.initials: PD
authors.lastname: Kerr
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics*; D004812:Epidemiologic Methods; D005260:Female; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*
chemical_list: D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human