title: Electrophoresis karyotype and chromosome-length polymorphism of Histoplasma capsulatum clinical isolates from Latin America.
abstract: Intact chromosomes of 19 clinical isolates of Histoplasma capsulatum recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed-field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5-7 bands, ranging from 1.3 to 10 Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7 Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of H. capsulatum varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source.
authors.affiliation: Departamento Micología, INEI ANLIS Dr. Carlos G. Malbrán, Buenos Aires, Argentina. ccanteros@anlis.gov.ar
authors.forename: Cristina Elena
authors.initials: CE
authors.lastname: Canteros
authors.forename: María Fernanda
authors.initials: MF
authors.lastname: Zuiani
authors.forename: Viviana
authors.initials: V
authors.lastname: Ritacco
authors.forename: Diego E
authors.initials: DE
authors.lastname: Perrotta
authors.forename: María Rocío
authors.initials: MR
authors.lastname: Reyes-Montes
authors.forename: Julio
authors.initials: J
authors.lastname: Granados
authors.forename: Gerardo
authors.initials: G
authors.lastname: Zúñiga
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Taylor
authors.forename: Graciela
authors.initials: G
authors.lastname: Davel
mesh_terms: D001118:Argentina; D002871:Chromosome Banding; D015825:Chromosomes, Fungal / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006166:Guatemala; D006658:Histoplasma / Q000235:genetics / Q000302:isolation & purification; D006660:Histoplasmosis / Q000382:microbiology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007621:Karyotyping / Q000379:methods*; D007843:Latin America; D008800:Mexico; D011110:Polymorphism, Genetic