title: Amino acid sequences of ferredoxins from Atropa belladonna and Hyoscyamus niger: their similarities to those in other tropane-alkaloid-containing plants.
abstract: The complete amino acid sequences of [2Fe-2S] ferredoxin from Atropa belladonna and Hyoscyamus niger have been determined by automated Edman degradation of the entire S-carboxymethylcysteinyl proteins and of the peptides obtained by enzymatic digestion. These two ferredoxins exhibited 1-8 differences in their amino acid sequences compared to those of other tropane-alkaloid-containing plants (Scopolia japonica, Datura stramonium, D. metel, and D. arborea), and only 1 or 4 differences compared to S. japonica and D. arborea. In contrast, 9-23 differences were observed among the other solanaceous ferredoxins. This suggests that tropane-alkaloid-containing plants are closely related taxonomically.
authors.affiliation: Department of Environment Analysis, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. mino@gly.oups.ac.jp
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Mino
authors.forename: Mayumi
authors.initials: M
authors.lastname: Yukita
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hiratsuka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wariishi
mesh_terms: D000595:Amino Acid Sequence; D001532:Atropa belladonna / Q000737:chemistry* / Q000145:classification; D005288:Ferredoxins / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D032304:Hyoscyamus / Q000737:chemistry* / Q000145:classification; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D005288:Ferredoxins