title: Evaluation of reduced toxicity of zearalenone by extrusion processing as measured by the MTT cell proliferation assay.
abstract: The objective of this study was to determine loss of toxicity of zearalenone in extruded cereal-based products by the MTT (tetrazolium salt) cell proliferation assay using a sensitive MCF-7 human breast cancer cell line and to compare the results to chemical (high-performance liquid chromatography, HPLC) and biochemical (enzyme-linked immunosorbent assay, ELISA) methods of analysis. A split-split plot design was used for the extrusion process experiments at temperatures of 150, 175, and 200 degrees C and screw speeds of 70 and 140 rpm. The initial zearalenone concentration in the artificially contaminated corn grits with Fusarium graminearum was found at a mean concentration of 37.88 microg/g as measured by HPLC. The percent reductions of zearalenone in the contaminated corn grits upon extrusion processing were in the ranges of 67-81, 60-72, and 66-78% as measured by HPLC, ELISA, and the MTT cell proliferation assay, respectively. The MTT cell proliferation assay results were more closely correlated with HPLC results (r = 0.96) than ELISA results (r = 0.83). The MTT cell proliferation assay was demonstrated to be a useful method for quantification of zearalenone as well as a potential toxicity screening method for contaminated extruded cereal-based products.
authors.affiliation: Department of Food Science and Technology, University of Nebraska-Lincoln, 143 Filley Hall, Lincoln, Nebraska 68583-0919, USA.
authors.forename: Yuksel
authors.initials: Y
authors.lastname: Cetin
authors.forename: Lloyd B
authors.initials: LB
authors.lastname: Bullerman
mesh_terms: D001943:Breast Neoplasms; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents; D002523:Edible Grain / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005511:Food Handling / Q000379:methods*; D006801:Humans; D013778:Tetrazolium Salts; D013844:Thiazoles; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D015025:Zearalenone; C022616:thiazolyl blue