title: Iteratively acting glycosyltransferases involved in the hexasaccharide biosynthesis of landomycin A.
abstract: Detailed studies on the biosynthesis of the hexasaccharide side chain of landomycin A, produced by S. cyanogenus S136, revealed the function of each glycosyltransferase gene of the biosynthetic gene cluster. Analyses of generated mutants as well as feeding experiments allowed us to determine that LanGT2 and LanGT3 catalyze the attachment of one sugar, whereas LanGT1 and LanGT4 attach two sugars during landomycin A biosynthesis. The generation of a lanZ2 deletion mutant provided evidence that LanZ2 is controlling the elongation of the saccharide side chain.
authors.affiliation: Albert-Ludwigs-Universität, Institut für Pharmazeutische Wissenschaften, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
authors.forename: Andriy
authors.initials: A
authors.lastname: Luzhetskyy
authors.forename: Marta
authors.initials: M
authors.lastname: Fedoryshyn
authors.forename: Clemens
authors.initials: C
authors.lastname: Dürr
authors.forename: Takaaki
authors.initials: T
authors.lastname: Taguchi
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Novikov
authors.forename: Andreas
authors.initials: A
authors.lastname: Bechthold
mesh_terms: D000617:Aminoglycosides / Q000096:biosynthesis* / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002384:Catalysis; D008401:Gas Chromatography-Mass Spectrometry; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D011134:Polysaccharides; C064648:landomycin A; D016695:Glycosyltransferases