[Quantitative analysis of pulegone in Herba Schizonepetae].
OBJECTIVE
To establish the determination method of pulegone in Herba Schizonepetae.


METHODS
HPLC method was developed for the determination of pulegone in Herba Schizonepetae. The separation was performed on C18 column with MeOH-H2O (80:20) as a mobile phase. The detection wavelength was set at 252 nm.


RESULTS
The quantitation of pulegone in Herba Schizonepetae varied from 0.2-0.7 mg x g(-1). The average recovery is 96.2%, RSD 0.81%.


CONCLUSIONS
The method is simple, rapid and accurate.
Yang
Jian-ping
JP
Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Bao
Bei-hua
BH
Zhang
Li
L
Ding
An-wei
AW
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000081005:Cyclohexane Monoterpenes; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019686:Lamiaceae / Q000737:chemistry*; D039821:Monoterpenes / Q000032:analysis*; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results
D000081005:Cyclohexane Monoterpenes; D004365:Drugs, Chinese Herbal; D039821:Monoterpenes; C039648:pulegone