Mist trickling bioreactor for Centaurium erythraea Rafn growth of shoots and production of secoiridoids.
Shoots of Centaurium erythraea Rafn were cultivated in 5 l mist trickling bioreactor for 21 and 28 days increasing their dry weight from 0.54 g to 13.7 g and 18.3 g, respectively. About 6880 shoots from 223 initial shoot-tips in 21-day bioreactor producing cycle were produced. The shoots could be successfully rooted and transferred to soil. Secoiridoid accumulation (expressed as a sum of gentiopicroside, sweroside and swertiamarin) in shoots after 21 days of culture reached about 303 mg l-1.
Piatczak
Ewelina
E
Department of Biology and Pharmaceutical Botany, Medical University, Kochanowskiego Str. 10, 51-601, Wroclaw, Poland. piatczak@biol.am.wroc.pl
Chmiel
Aleksander
A
Wysokinska
Halina
H
D019149:Bioreactors; D029966:Centaurium / Q000254:growth & development / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques / Q000379:methods; D039823:Iridoids / Q000032:analysis / Q000378:metabolism*; D010936:Plant Extracts / Q000032:analysis; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
D039823:Iridoids; D010936:Plant Extracts