title: Choristoneura fumiferana Granulovirus pk-1: a baculoviral protein kinase.
abstract: Open reading frame (ORF) 3 on the Choristoneura fumiferana granulovirus (ChfuGV), located in the 11 kb fragment of the BamHI genomic bank encodes a predicted 32-kDa putative kinase protein. Bioinformatics analysis on the predicted amino acid sequence of ChfuGV PK-1 revealed the existence of 11 catalytic subdomains. Sequence analysis within the 5'-untranslated region (5'-UTR) of ChfuGV pk- 1 indicates the presence of both putative early and late promoter motifs, indicating that pk-1 may be expressed throughout the infection cycle. Promoter sequence analysis reveals that pk-1 is deprived of a TATA box and appears instead to be regulated by other cis-acting transcriptional regulatory elements. Temporal transcription analysis by RT-PCR confirms the appearance of transcripts detected from 2 h p.i. until 72 h p.i. Northern blot hybridization characterizes pk-1 transcription as a 1.2 kb transcript. Homology comparisons reveal that ChfuGV PK-1 protein is most closely related to Phthorimaea operculalla granulovirus (PoGV) with 80 % amino acid identity.
authors.affiliation: Institut national de la recherche scientifique-Institut Armand-Frappier, Laval, Canada.
authors.forename: Paresa N
authors.initials: PN
authors.lastname: Giannopoulos
authors.forename: Nasha
authors.initials: N
authors.lastname: Nassoury
authors.forename: Lucie
authors.initials: L
authors.lastname: Lamontagne
authors.forename: Claude
authors.initials: C
authors.lastname: Guertin
authors.forename: Kianoush Khajeh
authors.initials: KK
authors.lastname: Rashidan
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D025722:Granulovirus / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000737:chemistry* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D011494:Protein Kinases