A monoclonal antibody-based ELISA allows discrimination between responses induced by bovine herpesvirus subtypes 1 (BoHV-1.1) and 2 (BoHV-1.2).
Bovine herpesvirus type 1 (BoHV-1) has distinct subtypes according to genomic characterization. Immune responses induced by BoHV-1 subtype 1 (BoHV-1.1) are not distinguishable from those induced by BoHV-1 subtype 2 (BoHV-1.2) through conventional serological methods. In the present report, an enzyme linked immunosorbent assay is described that allows discrimination between immune responses in cattle immunized with either subtype, based on a monoclonal antibody that recognizes specifically the amino-terminal region of glycoprotein C (gC) on BoHV-1.1 strains, thus not reacting with BoHV-1.2a. The test displayed a sensitivity of 92%, specificity of 90% and a good correlation with serum neutralization tests on samples from BoHV-1.1-immunized calves (kappa = 0.799). The test may be useful to provide new insights into the roles played by each of these two subtypes in the epidemiology of BoHV-1 infections.
Spilki
Fernando Rosado
FR
Centro de Pesquisas Veterinárias Desidério Finamor (CPVDF/FEPAGRO), Eldorado do Sul, Brazil. spilki@unicamp.br
Esteves
Paulo Augusto
PA
da Silva
Alessandra D'Avila
AD
Franco
Ana Cláudia
AC
Rijsewijk
Frans A M
FA
Roehe
Paulo Michel
PM
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000918:Antibody Specificity; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006561:Herpes Simplex / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology*; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D001911:Herpesvirus 2, Bovine / Q000276:immunology*; D007114:Immunization; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology*; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins