An in vitro evaluation of the cytotoxicity of various endodontic irrigants on human gingival fibroblasts.
The purpose of this study was to measure the cytotoxicity of six endodontic irrigants on cultured gingival fibroblasts using the CyQuant assay. Human gingival fibroblasts were grown in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum at 37 degrees C and 5% CO(2). At confluence, cells were split, plated in 96-well plates and incubated for 24-h to allow attachment. The following irrigants were tested at various concentrations: Sodium hypochlorite (NaOCl); iodine potassium-iodide (IKI); Betadine scrub (BS); calcium hydroxide [Ca(OH)2]; chlorine dioxide (SCD) and DMEM (positive control). Experimental groups were compared by the logarithmic difference between the clinical and LD50 concentrations of a particular irrigant. The results showed that IKI and Ca(OH)2 were significantly less cytotoxic than SCD, NaOCl, and BS. In conclusion, IKI and Ca(OH)2 are well tolerated by human gingival fibroblasts.
Barnhart
Brian D
BD
U.S. Army Endodontic Residency Program, Fort Gordon, GA 30905, USA.
Chuang
Augustine
A
Lucca
Jurandir J Dalle
JJ
Roberts
Steven
S
Liewehr
Frederick
F
Joyce
Anthony P
AP
D000704:Analysis of Variance; D002126:Calcium Hydroxide / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D017606:Chlorine Compounds / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D017613:Iodine Compounds / Q000633:toxicity; D007928:Lethal Dose 50; D010087:Oxides / Q000633:toxicity; D011206:Povidone-Iodine / Q000633:toxicity; D012388:Root Canal Irrigants / Q000633:toxicity*; D012973:Sodium Hypochlorite / Q000633:toxicity
D017606:Chlorine Compounds; D017613:Iodine Compounds; D010087:Oxides; D012388:Root Canal Irrigants; C439385:iodine potassium iodide; C025109:chlorine dioxide; D011206:Povidone-Iodine; D012973:Sodium Hypochlorite; D002126:Calcium Hydroxide