title: Presence of the P2X(7) purinergic receptor on immune cells that invade the rat endometrium during oestrus.
abstract: Eosinophils, macrophages and other leucocytes invade the uterine endometrium during oestrus and play a role in the tissue remodeling and immune responses that occur prior to implantation of the fertilized ovum. Adenosine 5'-triphosphate (ATP) and its metabolites influence uterine function via ATP receptors. In this study, we investigated the presence and localisation of the P2X(7) nucleotide receptor in the cells that infiltrate the uterine endometrium of adult female rats during oestrus at the electron microscope level, using gold-silver pre-embedding immunocytochemical techniques. P2X(7) receptor expression was found in the cytoplasm and the cell membrane of eosinophils, macrophages and fibroblasts in the endometrium during oestrus. These results suggest that ATP-mediated responses may be important in uterine preparation and remodeling before implantation and that this may involve several types of cells. In particular, the presence of P2X(7) receptors on endometrial stromal cells may indicate their involvement in apoptosis and immune and inflammatory responses.
authors.affiliation: Department of Anatomy, Christian Medical College, Vellore 632002, India.
authors.forename: Rachel
authors.initials: R
authors.lastname: Koshi
authors.forename: Robson
authors.initials: R
authors.lastname: Coutinho-Silva
authors.forename: Cynthia Machado
authors.initials: CM
authors.lastname: Cascabulho
authors.forename: Andrea
authors.initials: A
authors.lastname: Henrique-Pons
authors.forename: Gillian E
authors.initials: GE
authors.lastname: Knight
authors.forename: Andrzej
authors.initials: A
authors.lastname: Loesch
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Burnstock
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D004717:Endometrium / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D004804:Eosinophils / Q000737:chemistry* / Q000648:ultrastructure; D004971:Estrus / Q000276:immunology*; D005260:Female; D010539:Permeability / Q000187:drug effects; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000032:analysis*; D058486:Receptors, Purinergic P2X7; D013154:Spleen / Q000166:cytology
chemical_list: C546470:P2rx7 protein, rat; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; D000255:Adenosine Triphosphate