title: Structure-activity relationships of Erwinia carotovora quorum sensing signaling molecules.
abstract: Production of virulence factors and secondary metabolites is regulated in the phytopathogen Erwinia carotovora by quorum sensing involving N-acylated homoserine lactone (AHL) signaling molecules. Non-hydrolyzable AHL analogues were synthesized and screened in vivo. The biological activity of each compound was correlated with its ability to bind Erwinia AHL receptor proteins (LuxR homologues) in vitro. There is an excellent correlation between carbapenem production in vivo and in vitro binding to CarR. However, no such correlation could be found between exoprotease production and analogue binding to EccR. Our data are consistent with the involvement of a third, as yet uncharacterized LuxR homologue.
authors.affiliation: Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK.
authors.forename: Martin
authors.initials: M
authors.lastname: Welch
authors.forename: Jenny M
authors.initials: JM
authors.lastname: Dutton
authors.forename: Freija G
authors.initials: FG
authors.lastname: Glansdorp
authors.forename: Gemma L
authors.initials: GL
authors.lastname: Thomas
authors.forename: Debra S
authors.initials: DS
authors.lastname: Smith
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Coulthurst
authors.forename: Anne M L
authors.initials: AM
authors.lastname: Barnard
authors.forename: George P C
authors.initials: GP
authors.lastname: Salmond
authors.forename: David R
authors.initials: DR
authors.lastname: Spring
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis; D001665:Binding Sites; D015780:Carbapenems / Q000096:biosynthesis; D015169:Colony Count, Microbial; D020689:Exopeptidases / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects; D016973:Pectobacterium carotovorum / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D012097:Repressor Proteins; D015534:Trans-Activators; C064778:LuxR autoinducer binding proteins; C088386:homoserine lactone; D020689:Exopeptidases; D015107:4-Butyrolactone