title: Gram-scale chromatographic purification of beta-sitosterol. Synthesis and characterization of beta-sitosterol oxides.
abstract: An effective purification method for beta-sitosterol was developed starting from a commercial source of a phytosterol mixture using preparative adsorption column chromatography. beta-Sitosterol (> or = 95% purity) was obtained on a gram-scale. Thus, the synthesis of six beta-sitosterol oxides, including 7alpha-hydroxy, 7beta-hydroxy, 5,6alpha-epoxy, 5,6beta-epoxy, 7-keto, and 5alpha,6beta-dihydroxysitosterol, were successfully carried out. The spectral characteristics of all the synthetic intermediates and target compounds (approximately 95% purity) were well-documented.
authors.affiliation: Laboratoire de Chimie Analytique et Sciences de l'Aliment (UMR 7512), Faculté de Pharmacie, Université Louis Pasteur, 74 route du Rhin, 67400 Illkirch, France.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Philippe
authors.initials: P
authors.lastname: Geoffroy
authors.forename: Michel
authors.initials: M
authors.lastname: Miesch
authors.forename: Diane
authors.initials: D
authors.lastname: Julien-David
authors.forename: Francis
authors.initials: F
authors.lastname: Raul
authors.forename: Dalal
authors.initials: D
authors.lastname: Aoudé-Werner
authors.forename: Eric
authors.initials: E
authors.lastname: Marchioni
mesh_terms: D002845:Chromatography; D010087:Oxides / Q000138:chemical synthesis*; D010840:Phytosterols / Q000302:isolation & purification; D012855:Sitosterols / Q000737:chemistry* / Q000302:isolation & purification*; D013057:Spectrum Analysis
chemical_list: D010087:Oxides; D010840:Phytosterols; D012855:Sitosterols; C025473:gamma-sitosterol