Metabolism of fatty acid in yeast: characterisation of beta-oxidation and ultrastructural changes in the genus Sporidiobolus sp. cultivated on ricinoleic acid methyl ester.
Cell structure modifications and beta-oxidation induction were monitored in two strains of Sporidiobolus, Sp. Ruinenii and Sp. pararoseus after cultivation on ricinoleic acid methyl ester. Ultrastructural observations of the yeast before and after cultivation on fatty acid esters did not reveal major modifications in Sp. ruinenii. Unexpectedly, in Sp. pararoseus a proliferation of the mitochondrion was observed. After induction, Sp. ruinenii principally exhibited an increase in the activities of acyl-CoA oxidase (ACO), hydroxyacyl-CoA deshydrogenase (HAD), thiolase and catalase. In contrast, Sp. pararoseus lacked ACO and catalase activities, but an increase in acyl-CoA deshydrogenase (ACDH) and enoyl-CoA hydratase (ECH) activity was observed. These data suggest that in Sp. ruinenii, beta-oxidation is preferentially localized in the microbody, whereas in Sp. pararoseus it might be localized in the mitochondria.
Feron
Gilles
G
Laboratoire de Microbiologie, UMR INRA-UB 1232, 17 rue Sully, BP 86510, 21065 Dijon Cedex, France. feron@arome.dijon.inra.fr
Blin-Perrin
Caroline
C
Krasniewski
Isabelle
I
Mauvais
Geneviève
G
Lherminier
Jeannine
J
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D042962:Acyl-CoA Oxidase / Q000378:metabolism; D000217:Acyltransferases / Q000378:metabolism; D001487:Basidiomycota / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure*; D002374:Catalase / Q000378:metabolism; D002451:Cell Compartmentation; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D007700:Kinetics; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D010084:Oxidation-Reduction; D012277:Ricinoleic Acids / Q000378:metabolism*; D013045:Species Specificity
D005227:Fatty Acids; D012277:Ricinoleic Acids; C005235:methyl ricinoleate; D015094:3-Hydroxyacyl CoA Dehydrogenases; D002374:Catalase; D042962:Acyl-CoA Oxidase; D000217:Acyltransferases; D004746:Enoyl-CoA Hydratase