An alternative method to prepare samples of the pathogenic yeast Cryptococcus neoformans for scanning electron microscopy analysis.
In this work, an alternative to conventional preparation procedures for scanning electron microscopy (SEM) analysis of Cryptococcus neoformans was performed. The cells were fixed directly in the agar culture. This method is simpler than others already reported and the morphology of the cells was well preserved.
Faganello
Josiane
J
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, 91501-970, Porto Alegre, RS, Brazil.
Arruda
Walquíria
W
Schrank
Augusto
A
Vainstein
Marilene Henning
MH
D000362:Agar; D003455:Cryptococcus neoformans / Q000648:ultrastructure*; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry
D011134:Polysaccharides; D000362:Agar