4-Dihydromethyltrisporate dehydrogenase, an enzyme of the sex hormone pathway in Mucor mucedo, is constitutively transcribed but its activity is differently regulated in (+) and (-) mating types.
4-Dihydromethyltrisporate dehydrogenase (TDH) converts the (+) mating type sex pheromone 4-dihydromethyltrisporate into methyltrisporate. In Mucor mucedo, this conversion is required only in the (-) mating type. Expression of the TDH encoding TSP1 gene was analyzed qualitatively using reverse-transcribed PCR. TSP1 is constitutively transcribed in the (+) and in the (-) mating type, irrespective of the mating situation. By immunodetection, the translation product is also formed constitutively. In contrast to gene expression, TDH enzyme activity depends on the sexual status of the mycelium. Activity is restricted to the sexually stimulated (-) mating type. Non-stimulated (-), as well as stimulated and non-stimulated (+) mycelia exhibit no activity and do not influence activity in stimulated (-) mycelia. Time course analysis shows strongly increased enzyme activity at 80 min after stimulation. Low activity exists from the onset of stimulation, indicating that additional regulation mechanisms are involved in TDH function.
Schimek
Christine
C
Department of General Microbiology and Microbial Genetics, Institute of Microbiology, Friedrich-Schiller-University Jena, Neugasse 24, D-07743 Jena, Germany. b9SCCR@uni-jena.de
Petzold
Annett
A
Schultze
Kornelia
K
Wetzel
Jana
J
Wolschendorf
Frank
F
Burmester
Anke
A
Wöstemeyer
Johannes
J
D015153:Blotting, Western; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D009089:Mucor / Q000201:enzymology* / Q000235:genetics*; D025282:Mycelium / Q000201:enzymology; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger; D010088:Oxidoreductases; C500532:dihydromethyl-trisporate dehydrogenase