The effect of norflurazon on protein composition and chlorophyll organization in pigment-protein complex of photosystem II.
The pyridazinone-type herbicide norflurazon SAN 9789 inhibiting the biosynthesis of long-chain carotenoids results in significant decrease in PS II core complexes and content of light-harvesting complex (LHC) polypeptides in the 29.5-21 kDa region. The Chl a forms at 668, 676, and 690 nm that belong to LHC and antenna part of PS I disappear completely after treatment. The intensity of the Chl b form at 648 nm is sharply decreased in treated seedlings grown under 30 or 100 lx light intensity. The bands of carotenoid absorption at 421, 448 (Chl a), 452, 480, 492, 496 (beta-carotene), and 508 nm also disappear. The band shift from 740 to 720 nm and decrease in its intensity relative to the 687 nm emission peak in the low-temperature fluorescence spectrum (77 K) suggests a disturbance of energy transfer from LHC to the Chl a form at 710-712 nm.
Guseinova
Irada M
IM
Department of Molecular-Genetic Bases of Production Processes, Institute of Botany, Azerbaijan National Academy of Sciences, Patamdar Shosse 40, 1073 Baku, Azerbaijan.
Suleimanov
Saftar Y
SY
Aliyev
Jalal A
JA
D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D006540:Herbicides / Q000494:pharmacology*; D008027:Light; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism*; D011724:Pyridazines / Q000494:pharmacology*; D014908:Triticum / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism
D006540:Herbicides; D045332:Photosystem II Protein Complex; D011724:Pyridazines; D002734:Chlorophyll; D002338:Carotenoids; C008677:norflurazone