Vanadium (V)-induced neurotoxicity in the rat central nervous system: a histo-immunohistochemical study.
As vanadium was found to induce oxidative stress in the central nervous system, the morphological alterations of neurons and astroglial cells in adult rat central nervous system after vanadium exposure was studied, using histological markers of cellular injury. Animals were intraperitoneally injected with 3 mg/kg body weight of sodium metavanadate for 5 consecutive days. NADPH diaphorase histochemistry and heat shock protein (hsp) 70, glial fibrillary acidic protein (GFAP), and S-100 immunohistochemistry were performed in floating sections of several brain areas. NADPHd staining was higher in the molecular and granular layers of the cerebellar cortex, and small NADPHd-stained interneurons were observed in hippocampal sections in V(+5)-exposed animals. hsp 70 immunostaining showed the presence of reactive neurons in cerebellum of treated animals. GFAP and S-100 immunohistochemistry showed enlarged astrocytes in cerebellum and hippocampus in the V(+5)-exposed animals. The histological markers used showed that the main areas affected by vanadium-mediated free-radical generation were the hippocampus and the cerebellum.
Garcia
Graciela Beatriz
GB
Morphology Department, School of Biochemical and Pharmaceutical Sciences, National University of Rosario, Santa Fe, Argentina. ggarcia@fbioyf.unr.edu.ar
Biancardi
Maria Eugenia
ME
Quiroga
Ariel Dario
AD
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000473:pathology; D002490:Central Nervous System / Q000473:pathology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D020258:Neurotoxicity Syndromes / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D009418:S100 Proteins / Q000096:biosynthesis; D014638:Vanadates / Q000633:toxicity*
D005904:Glial Fibrillary Acidic Protein; D018840:HSP70 Heat-Shock Proteins; D009418:S100 Proteins; D014638:Vanadates; D009252:NADPH Dehydrogenase