Chemiluminescence biosensor system for lactic acid using natural animal tissue as recognition element.
A new method based on natural animal tissue porcine kidney as recognition element for chemiluminescence sensing of lactic acid is proposed in this paper. The principle for lactic acid sensing is that lactic acid is oxidized by oxygen under the catalysis of alpha-hydroxy acid oxidase in the tissue column to produce hydrogen peroxide, which can react with luminol in the presence of potassium ferricyanide to generate a CL signal. The experimental results show that the CL emission intensity was linear with lactic acid concentration in the range of 1-1000 micromol/L and the detection limit (3sigma) for lactic acid was 0.2 micromol/L. The biosensor could be used continuously for 6h with no significant changes in the response. More than 240 measurements were carried out during this time. A complete analysis, including sampling and washing, could be performed in 1.5 min with a relative standard deviation of 1.12% for 100 micromol/L lactic acid. The reproducibility among tissue columns was satisfactory (RSD among columns is less than 5%). The biosensor has been applied successfully to the analysis of lactic acid in plasma and milk samples.
Wu
Fangqiong
F
College of Chemistry and Chemical Engineering, Chongqing Key Laboratory of Modern Analytical Sciences, Southwest Normal University, Beibei, Chongqing 400715, PR China.
Huang
Yuming
Y
Huang
Chengzhi
C
D000818:Animals; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007668:Kidney / Q000187:drug effects* / Q000502:physiology; D019344:Lactic Acid / Q000032:analysis* / Q000494:pharmacology*; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D008892:Milk / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D046509:Tissue Culture Techniques / Q000295:instrumentation* / Q000379:methods
D019344:Lactic Acid