Buffer solution can control the porosity of DNA-chitosan complexes.
The current studies examine the pore properties and biological effects of DNA-chitosan complexes, which may be useful as scaffolds for tissue engineering. The porosity of the DNA-chitosan complexes was controlled by rinsing them with several different pH 7.2 buffer solutions, including phosphate-buffered saline (PBS), Tris-HCl, boric acid, and N-(2-hydroxyethyl)piperazine-N'-(2-ethanesufonic acid) (HEPES). Rinsing with PBS resulted in 84% porosity, whereas rinsing with Tris-HCl produced 94% porosity. It was further found that daunorubicin hydrochloride complex intercalated with and bound to the groove of the DNA-chitosan complexes, indicating that DNA in the complexes maintains its double-stranded helical structure. The DNA-chitosan complexes were not toxic to MG-63 osteoblast-like cells and caused only a mild tissue response when implanted subcutaneously in the backs of rats. These results suggest that buffer-rinsed DNA-chitosan complexes may be useful as a scaffold material in tissue engineering.
Fukushima
Tadao
T
Bioengineering Section, Department of Dental Engineering, Fukuoka Dental College, Sawara-ku, Fukuoka 814-0193, Japan. tadaof@college.fdcnet.ac.jp
Hayakawa
Tohru
T
Okamura
Kazuhiko
K
Takeda
Shoji
S
Inoue
Yusuke
Y
Miyazaki
Koji
K
Okahata
Yoshio
Y
D002021:Buffers; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts
D002021:Buffers; D004247:DNA; D048271:Chitosan