Micellar electrokinetic capillary chromatography analysis of phthalic acid isomers.
A capillary electrophoretic method for the analysis and separation of ortho- and para-phthalic acid isomers was developed. The best separation was achieved using micellar electrokinetic capillary chromatography (MECC) performed in a 110 mM borate buffer pH 8.3 also containing 40 mM sodium dodecyl sulfate (SDS) and 20% methanol. The effect of the addition of methanol, acetonitrile, and beta-cyclodextrin on the separation of the isomers was investigated. The buffer system developed was shown to provide a dependable method of analysis from which the concentration of each analyte could be reliably determined.
Rigout
Muriel L A
ML
Colour and Polymer Chemistry Department, University of Leeds, Leeds, West Yorkshire, UK.
Lewis
David M
DM
Broadbent
Peter J
PJ
D002853:Chromatography, Liquid / Q000379:methods*; D003102:Colloids / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D007700:Kinetics; D008823:Micelles; D010795:Phthalic Acids / Q000032:analysis* / Q000737:chemistry*
D003102:Colloids; D008823:Micelles; D010795:Phthalic Acids; C032279:phthalic acid