title: Region-specific alterations of adenosine receptors expression level in kidney of diabetic rat.
abstract: Pathological alterations of renal function in insulin-dependent diabetes have been attributed to numerous factors, including adenosine. This study examined the expression levels of adenosine receptors (ARs) in the kidney of the streptozotocin-induced diabetic rat. In the diabetic kidney A1-AR mRNA levels increased 1.7- and 2.8-fold in cortex and medulla, respectively. This was accompanied by increased A1-AR protein levels in membranes of kidney cortex (1.5-fold) and medulla (threefold). A1-AR immunoreactivity increased strongly along medullar tubules especially in the collecting duct. The levels of A2a-AR mRNA increased twofold in diabetic kidney cortex but remained unchanged in medulla; however, A2a-AR protein levels increased more than threefold in cortex. Immunohistochemistry showed increased A2a-AR immunoreactivity in luminal membranes of cortical collecting ducts and in epithelial cells of preglomerular vessels. There were no significant changes in A2b-AR expression in diabetic kidney except in medullar membranes, where the receptor protein content decreased by 60%. A3-AR mRNA levels in diabetic kidney remained unchanged, but membrane-associated A3-AR protein levels increased by 70% in diabetic kidney cortex and decreased by 80% in medulla. These changes in ARs genes expression, receptor protein content, and cellular and tissue distribution, correspond to abnormalities characteristic of the diabetic kidney, suggesting involvement in pathogenesis of diabetic nephropathy.
authors.affiliation: Department of Molecular Medicine, Medical University of Gdansk, ul. Debinki 7, paw. 29, 80-211 Gdansk, Poland. tkpaw@amg.gda.pl
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Pawelczyk
authors.forename: Marzena
authors.initials: M
authors.lastname: Grden
authors.forename: Robert
authors.initials: R
authors.lastname: Rzepko
authors.forename: Monika
authors.initials: M
authors.lastname: Sakowicz
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szutowicz
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D007672:Kidney Cortex / Q000378:metabolism* / Q000473:pathology; D007679:Kidney Medulla / Q000378:metabolism* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018047:Receptors, Purinergic P1 / Q000235:genetics / Q000378:metabolism*; D013311:Streptozocin; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D018047:Receptors, Purinergic P1; D013311:Streptozocin