[Antigen analysis of Angiostrongylus cantonensis in different developmental stages].
OBJECTIVE
To analyze the difference among antigens of Angiostrongylus cantonensis in different developmental stages and identify dominant diagnostic antigen for angiostrongyliasis.


METHODS
Antigens of A. cantonensis in different developmental stages were analyzed by SDS-PAGE and immunoblot.


RESULTS
The protein bands of all developmental stages were similar on SDS-PAGE. The Mr 40000, 50000, 66000 and 80000 antigens reacted not only with the sera of rats infected by A. cantonensis but also with the sera of normal rats. The Mr 104000 antigen could be discerned by sera of rats infected with A. cantonensis for 2 weeks. The Mr 32000 antigen could be recognized by sera of rats 2 weeks after infection, and the reaction became stronger with the infection continued.


CONCLUSIONS
The Mr 40000, 50000, 66000 and 80000 antigens might result in the unspecific reaction in the immunodiagnosis of angiostrongyliasis using the crude antigen of A. cantonensis. The Mr 104000 of larva, Mr 33000 of adult females and Mr 32000 of the worms might be used as candidate antigens in early diagnosis and epidemiological survey of angiostrongyliasis.
Li
Hua
H
Department of Parasitology, Southern Medical University, Guangzhou 510515, China.
Chen
Xiao-guang
XG
Shen
Hao-xian
HX
Peng
Hong-juan
HJ
Zhao
Xing-cun
XC
D017185:Angiostrongylus cantonensis / Q000254:growth & development / Q000276:immunology*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D051381:Rats; D017207:Rats, Sprague-Dawley; D017206:Strongylida Infections / Q000175:diagnosis / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth