title: New concepts in the chromatography of peptides and proteins.
abstract: Although chromatography using a variety of novel bed configurations (e.g. fluidized beds, expanded beds, simulated moving beds, annular rotating beds, etc.) has been of recent interest, the majority of practical applications of analytical and preparative chromatography employ a stationary adsorbent bed into which a feed slug is charged periodically, similar to the technique first described by Mikhail Tswett over 100 years ago. However, new concepts in both the practice and theory of fixed-bed chromatography are continuing to expand the available range of applications for separating peptides and proteins.
authors.affiliation: Department of Chemical and Biochemical Engineering, 1000 Hilltop Circle, University of Maryland Baltimore County, Baltimore, Maryland 21250, USA. dfrey1@umbc.edu
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Frey
authors.forename: Xuezhen
authors.initials: X
authors.lastname: Kang
mesh_terms: D000465:Algorithms; D002845:Chromatography / Q000379:methods* / Q000639:trends; D002846:Chromatography, Affinity / Q000379:methods / Q000639:trends; D004058:Diffusion; D007128:Immunoglobulin Fragments / Q000737:chemistry; D007135:Immunoglobulin Variable Region / Q000737:chemistry; D047668:Inteins / Q000235:genetics; D044085:Microfluidics; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D014783:Viscosity
chemical_list: D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D010455:Peptides; D011108:Polymers; D011506:Proteins