LSECtin interacts with filovirus glycoproteins and the spike protein of SARS coronavirus.
Cellular attachment factors like the C-type lectins DC-SIGN and DC-SIGNR (collectively referred to as DC-SIGN/R) can augment viral infection and might promote viral dissemination in and between hosts. The lectin LSECtin is encoded in the same chromosomal locus as DC-SIGN/R and is coexpressed with DC-SIGNR on sinusoidal endothelial cells in liver and lymphnodes. Here, we show that LSECtin enhances infection driven by filovirus glycoproteins (GP) and the S protein of SARS coronavirus, but does not interact with human immunodeficiency virus type-1 and hepatitis C virus envelope proteins. Ligand binding to LSECtin was inhibited by EGTA but not by mannan, suggesting that LSECtin unlike DC-SIGN/R does not recognize high-mannose glycans on viral GPs. Finally, we demonstrate that LSECtin is N-linked glycosylated and that glycosylation is required for cell surface expression. In summary, we identified LSECtin as an attachment factor that in conjunction with DC-SIGNR might concentrate viral pathogens in liver and lymph nodes.
Gramberg
Thomas
T
Institute for Clinical and Molecular Virology, University Erlangen-Nürnberg, Germany.
Hofmann
Heike
H
Möller
Peggy
P
Lalor
Patricia F
PF
Marzi
Andrea
A
Geier
Martina
M
Krumbiegel
Mandy
M
Winkler
Thomas
T
Kirchhoff
Frank
F
Adams
David H
DH
Becker
Stephan
S
Münch
Jan
J
Pöhlmann
Stefan
S
D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D042783:Endothelial Cells / Q000821:virology; D016563:Filoviridae / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007668:Kidney; D037181:Lectins, C-Type / Q000378:metabolism*; D008099:Liver / Q000166:cytology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D016296:Mutagenesis; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D045473:Severe acute respiratory syndrome-related coronavirus / Q000378:metabolism*; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
C505228:CLEC4G protein, human; D017931:DNA Primers; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus