Theoretical model of the interactions between Ca2+, calmodulin and myosin light chain kinase.
Active Ca2+/calmodulin (CaM)-dependent myosin light chain kinase (MLCK) plays an important role in the process of MLC phosphorylation and consecutive smooth muscle contraction. Here, we propose a mathematical model of a detailed kinetic scheme describing interactions among Ca2+, CaM and MLCK and taking into account eight different aggregates. The main model result is the prediction of the Ca2+ dependent active form of MLCK, which is in the model taken as proportional to the concentration of Ca4CaM.MLCK complex. Wegscheider's condition is additionally applied as a constraint enabling the prediction of some parameter values that have not yet been obtained by experiments.
Fajmut
Ales
A
Department of Physics, Medical Faculty and Faculty of Education, University of Maribor, Slomskov trg 15, SI-2000 Maribor, Slovenia. ales.fajmut@uni-mb.si
Brumen
Milan
M
Schuster
Stefan
S
D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism; D008962:Models, Theoretical; D018994:Myosin Light Chains / Q000378:metabolism; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D010766:Phosphorylation
D002147:Calmodulin; D002413:Cations, Divalent; D018994:Myosin Light Chains; D009219:Myosin-Light-Chain Kinase; D002118:Calcium