Cloning and characterization of hoxH genes from Arthrospira and Spirulina and application in phylogenetic study.
Partial hoxH genes of 2 cyanobacterial genera, including 5 strains of Arthrospira and 2 strains of Spirulina, were cloned and characterized. This gene encodes the large subunit of nickel-iron hydrogenase. The results showed that these genes comprised 1349 nucleotides in Arthrospira and 1343 nucleotides in Spirulina, respectively. The GC contents of hoxH were 45.7% to 47.3% in Arthrospira and up to 50.4% to 50.9% in Spirulina. The hoxH gene was demonstrated to be single copy by Southern analysis, and its transcription was verified by reverse transcriptase polymerase chain reaction in Arthrospira platensis FACHB341. The similarities of nucleotide sequences among the 5 strains of Arthrospira ranged from 95.7% to 99.8%, which are higher than those between Arthrospira and Spirulina (72.9-77.0%). However, similarity between the 2 Spirulina strains was only 72.5%, slightly lower than that between the 2 genera. A phylogenetic tree based on hoxH was constructed. All 5 strains of Arthrospira formed a monophyletic clade, which was highly supported by bootstrap value, while the 2 strains of Spirulina were separated into 2 different clades.
Zhang
Xiaohui
X
College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, Peoples Republic of China. xczhang@ouc.edu.cn
Zhang
Xuecheng
X
Shiraiwa
Yoshihiro
Y
Mao
Yunxiang
Y
Sui
Zhenghong
Z
Liu
Jinjie
J
D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D016000:Cluster Analysis; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D017931:DNA Primers; D005801:Genes, Homeobox / Q000235:genetics*; D006864:Hydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity
D017931:DNA Primers; C043296:nickel-iron hydrogenase; D006864:Hydrogenase