title: OD1, the first toxin isolated from the venom of the scorpion Odonthobuthus doriae active on voltage-gated Na+ channels.
abstract: In this study, we isolated and pharmacologically characterized the first alpha-like toxin from the venom of the scarcely studied Iranian scorpion Odonthobuthus doriae. The toxin was termed OD1 and its primary sequence was determined: GVRDAYIADDKNCVYTCASNGYCNTECTKNGAESGYCQWIGRYGNACWCIKLPDEVPIRIPGKCR. Using the two-electrode voltage clamp technique, the pharmacological effects of OD1 were studied on three cloned voltage-gated Na+ channels expressed in Xenopus laevis oocytes (Na(v)1.2/beta1, Na(v)1.5/beta1, para/tipE). The inactivation process of the insect channel, para/tipE, was severely hampered by 200 nM of OD1 (EC50 = 80+/-14 nM) while Na(v)1.2/beta1 still was not affected at concentrations up to 5 microM. Na(v)1.5/beta1 was influenced at micromolar concentrations.
authors.affiliation: Department of Toxicology and Pharmacology, Shaheed Beheshti University of Medical Science, Tehran, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Jalali
authors.forename: Frank
authors.initials: F
authors.lastname: Bosmans
authors.forename: Mehriar
authors.initials: M
authors.lastname: Amininasab
authors.forename: Elke
authors.initials: E
authors.lastname: Clynen
authors.forename: Eva
authors.initials: E
authors.lastname: Cuypers
authors.forename: Abbas
authors.initials: A
authors.lastname: Zaremirakabadi
authors.forename: Mohammad-Nabi
authors.initials: MN
authors.lastname: Sarbolouki
authors.forename: Liliane
authors.initials: L
authors.lastname: Schoofs
authors.forename: Hossein
authors.initials: H
authors.lastname: Vatanpour
authors.forename: Jan
authors.initials: J
authors.lastname: Tytgat
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D012604:Scorpion Venoms / Q000737:chemistry*; D012605:Scorpions; D017386:Sequence Homology, Amino Acid; D015222:Sodium Channels / Q000187:drug effects*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009498:Neurotoxins; D012604:Scorpion Venoms; D015222:Sodium Channels