Efficient transfection of DNA by liposomes formulated with cationic gemini amphiphiles.
Cationic liposomes formulated with neutral 1,2-dimyristoyl-sn-glycero-3-phosphocholine and cationic gemini surfactants were used for transfecting different cell lines with a reporter gene. The efficiency in the transfection has been correlated to the high extent of DNA condensation observed by circular dichroism, condensation shown to depend heavily on the gemini spacer structure. Transfection efficiency was better than that obtained with a commercial lipofection kit.
Bombelli
Cecilia
C
Istituto di Metodologie Chimiche del CNR and Dipartimento di Chimica, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
Faggioli
Francesca
F
Luciani
Paola
P
Mancini
Giovanna
G
Sacco
Maria Grazia
MG
D000818:Animals; D002082:Butylamines / Q000737:chemistry; D002412:Cations; D002460:Cell Line; D002522:Chlorocebus aethiops; D002942:Circular Dichroism; D003587:Cytomegalovirus / Q000235:genetics; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D005021:Ethylamines / Q000737:chemistry; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D011401:Promoter Regions, Genetic; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D051381:Rats; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000737:chemistry*; D014162:Transfection
D002082:Butylamines; D002412:Cations; D005021:Ethylamines; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D049452:Green Fluorescent Proteins; D004247:DNA; D004134:Dimyristoylphosphatidylcholine