title: The effect of preconditioning on the Cu, Zn superoxide dismutase expression and enzyme activity in rat brain at the early period after severe hypobaric hypoxia.
abstract: Severe hypoxia results in functional and structural injury of the brain. A preconditioning with repetitive episodes of mild hypoxia considerably ameliorates neuronal resistance to subsequent severe hypoxia. Activation of endogenous antioxidants including Cu, Zn-depending superoxide dismutase (Cu, Zn-SOD) (EC.1.15.1.1) is one of the main cell defense mechanisms against oxidative stress induced by hypoxia. Alterations of expression and enzyme activity of Cu, Zn-SOD 3 and 24h after severe hypobaric hypoxia in forebrain structures of preconditioned and non-preconditioned rats were investigated. We found that hypoxia without preconditioning suppressed the Cu, Zn-SOD enzyme activity at 3h time-point but preconditioning essentially modified the reaction to severe hypoxia by increasing the expression and activity of Cu, Zn-SOD during early stages of reoxygenation crucial for apoptosis initiation.
authors.affiliation: Department of Developmental Biology, Tampere University Medical School and Department of Pathology, Tampere University Hospital, Tampere FIN-33014, Finland.
authors.forename: Serguei A
authors.initials: SA
authors.lastname: Stroev
authors.forename: Tatjana S
authors.initials: TS
authors.lastname: Gluschenko
authors.forename: Ekaterina I
authors.initials: EI
authors.lastname: Tjulkova
authors.forename: Elena A
authors.initials: EA
authors.lastname: Rybnikova
authors.forename: Michail O
authors.initials: MO
authors.lastname: Samoilov
authors.forename: Markku
authors.initials: M
authors.lastname: Pelto-Huikko
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002452:Cell Count / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D000860:Hypoxia / Q000201:enzymology*; D007150:Immunohistochemistry / Q000379:methods; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*; D013997:Time Factors
chemical_list: D013482:Superoxide Dismutase