title: Analysis and frequency of bovine lymphocyte antigen (BoLA-DRB3) alleles in Iranian Holstein cattle.
abstract: The bovine lymphocyte antigen (BoLA-DRB3) gene encodes cell surface glycoproteins that initiate immune response by presenting processed antigenic peptides to CD4 T helper cells. DRB3 is the most polymorphic bovine MHC class II gene which encodes the peptide-binding groove. DRB3 gene has been extensively evaluated as a candidate marker for association with various bovine diseases and immunological traits. This study describes genetic variability in the BoLA-DRB3 in Iranian Holstein cattle. This is the first study of the DNA polymorphism of the BoLA-DRB3 gene in Iranian Holstein cattle. Hemi-nested PCR-RFLP method is used for identification the frequency of BoLA-DRB3 alleles. The BoLA-DRB3 locus is highly polymorphic in the studied herd (26 alleles). Almost 67% of the alleles were accounted for four alleles (BoLA-DRB3.2*8, *24, *11 and *16) in Iranian Holstein cattle. The DRB3.2*8 allele frequency (26.6%) was higher than the others. The frequencies of the DRB3.2*54, *37, *36, *28, *25, *14, *13, *10, *1 alleles were lower than 1%. Significant distinctions have been found between Iranian Holstein cattle and other cattle breeds studied. In Iranian Holstein cattle the alleles (BoLA-DRB3.2*22, *2 and *16) associated with a lower risk of cystic ovarian disease in Holstein cattle are found. The alleles associated with the resistance to mastitis and to bovine leukemia virus infection BoLA-DRB3.2*11 and *23 are detected with the frequencies 10.4% and 4.4%, respectively. Thus in the Iranian Holstein cows studied are found alleles which are associated with resistance to various diseases. The method of DNA-typing of animals can be used in agricultural practice for BoLA-DRB3 allele genotyping of cattle in order to reduce spreading of alleles providing susceptibility to mastitis or leukemia in cattle herds.
authors.affiliation: Center of Excellence in Animal Science, College ofAgriculture, Ferdowsi University of Mashhad, Mashhad 91775-1163, Iran. m_nassiry@yahoo.com
authors.forename: M R
authors.initials: MR
authors.lastname: Nassiry
authors.forename: F Eftekhar
authors.initials: FE
authors.lastname: Shahroodi
authors.forename: J
authors.initials: J
authors.lastname: Mosafer
authors.forename: A
authors.initials: A
authors.lastname: Mohammadi
authors.forename: E
authors.initials: E
authors.lastname: Manshad
authors.forename: S
authors.initials: S
authors.lastname: Ghazanfari
authors.forename: M R
authors.initials: MR
authors.lastname: Mohammad Abadi
authors.forename: G E
authors.initials: GE
authors.lastname: Sulimova
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D005787:Gene Frequency / Q000235:genetics* / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D007492:Iran; D007938:Leukemia / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D008297:Male; D008414:Mastitis, Bovine / Q000235:genetics / Q000276:immunology
chemical_list: C423282:BoLA-DRB3 antigen; D000949:Histocompatibility Antigens Class II