Antigen-specific and non-specific CD4+ T cell recruitment and proliferation during influenza infection.
To track epitope-specific CD4(+) T cells at a single-cell level during influenza infection, the MHC class II-restricted OVA(323-339) epitope was engineered into the neuraminidase stalk of influenza/A/WSN, creating a surrogate viral antigen. The recombinant virus, influenza A/WSN/OVA(II), replicated well, was cleared normally, and stimulated both wild-type and DO11.10 or OT-II TCR transgenic OVA-specific CD4(+) T cells. OVA-specific CD4 T cells proliferated during infection only when the OVA epitope was present. However, previously primed (but not naive) transgenic CD4(+) T cells were recruited to the infected lung both in the presence and absence of the OVA(323-339) epitope. These data show that, when primed, CD4(+) T cells may traffic to the lung in the absence of antigen, but do not proliferate. These results also document a useful tool for the study of CD4 T cells in influenza infection.
Chapman
Timothy J
TJ
Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, Aab Institute of Biomedical Sciences, University of Rochester Medical Center, NY 14642, USA.
Castrucci
Maria R
MR
Padrick
Ryan C
RC
Bradley
Linda M
LM
Topham
David J
DJ
D019264:Adoptive Transfer; D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D004195:Disease Models, Animal; D004285:Dogs; D009980:Influenza A virus / Q000302:isolation & purification; D007668:Kidney; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D010047:Ovalbumin / Q000737:chemistry; D010446:Peptide Fragments; D010948:Viral Plaque Assay
D010446:Peptide Fragments; D010047:Ovalbumin