title: Membrane blebbing in cancer cells treated with various apoptotic inducers.
abstract: The dynamics of cell morphology, in particular membrane blebbing, was studied after induction of apoptosis by etoposide or camptothecin in four human stabilized cell lines (Hep2, Bowes, HT-29 and HL-60). Time lapse videomicroscopy and F-actin staining revealed various dynamic parameters of this process including its duration, maximal extent, stages and final endpoints in individual cells. Although generally occurring in predictable patterns, our results indicate a relatively significant variability both in appearance and temporal organization of blebbing not only between different cell lines but also within them.
authors.affiliation: Charles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biology and Genetics, Hradec Králové, Czech Republic. rudolf@lfhk.cuni.cz
authors.forename: Emil
authors.initials: E
authors.lastname: Rudolf
authors.forename: Miroslav
authors.initials: M
authors.lastname: Cervinka
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000000981:diagnostic imaging / Q000187:drug effects; D002462:Cell Membrane / Q000000981:diagnostic imaging / Q000187:drug effects*; D003599:Cytoskeleton / Q000187:drug effects; D005047:Etoposide / Q000494:pharmacology*; D006801:Humans; D018715:Microscopy, Video; D014463:Ultrasonography
chemical_list: D000970:Antineoplastic Agents; D005047:Etoposide; D002166:Camptothecin