title: The first crystal structure of a family 31 carbohydrate-binding module with affinity to beta-1,3-xylan.
abstract: Here, we present the crystal structure of the family 31 carbohydrate-binding module (CBM) of beta-1,3-xylanase from Alcaligenes sp. strain XY-234 (AlcCBM31) determined at a resolution of 1.25A. The AlcCBM31 shows affinity with only beta-1,3-xylan. The AlcCBM31 molecule makes a beta-sandwich structure composed of eight beta-strands with a typical immunoglobulin fold and contains two intra-molecular disulfide bonds. The folding topology of AlcCBM31 differs from that of the large majority of other CBMs, in which eight beta-strands comprise a beta-sandwich structure with a typical jelly-roll fold. AlcCBM31 shows structural similarity with CBM structures of family 34 and family 9, which also adopt structures based on immunoglobulin folds.
authors.affiliation: International Graduate school of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan. hash@tsurumi.yokohama-cu.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Youichi
authors.initials: Y
authors.lastname: Tamai
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Okazaki
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamaru
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Toshiyoshi
authors.initials: T
authors.lastname: Araki
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sato
mesh_terms: D000421:Alcaligenes / Q000201:enzymology*; D000595:Amino Acid Sequence; D050260:Carbohydrate Metabolism; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D043325:Xylan Endo-1,3-beta-Xylosidase / Q000737:chemistry* / Q000378:metabolism; D014990:Xylans / Q000378:metabolism*
chemical_list: D014990:Xylans; C065917:1,3-xylan; D043325:Xylan Endo-1,3-beta-Xylosidase