title: The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses.
abstract: The completion of the 5,373,180-bp genome sequence of the marine psychrophilic bacterium Colwellia psychrerythraea 34H, a model for the study of life in permanently cold environments, reveals capabilities important to carbon and nutrient cycling, bioremediation, production of secondary metabolites, and cold-adapted enzymes. From a genomic perspective, cold adaptation is suggested in several broad categories involving changes to the cell membrane fluidity, uptake and synthesis of compounds conferring cryotolerance, and strategies to overcome temperature-dependent barriers to carbon uptake. Modeling of three-dimensional protein homology from bacteria representing a range of optimal growth temperatures suggests changes to proteome composition that may enhance enzyme effectiveness at low temperatures. Comparative genome analyses suggest that the psychrophilic lifestyle is most likely conferred not by a unique set of genes but by a collection of synergistic changes in overall genome content and amino acid composition.
authors.affiliation: The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. bmethe@tigr.org
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Methé
authors.forename: Karen E
authors.initials: KE
authors.lastname: Nelson
authors.forename: Jody W
authors.initials: JW
authors.lastname: Deming
authors.forename: Bahram
authors.initials: B
authors.lastname: Momen
authors.forename: Eugene
authors.initials: E
authors.lastname: Melamud
authors.forename: Xijun
authors.initials: X
authors.lastname: Zhang
authors.forename: John
authors.initials: J
authors.lastname: Moult
authors.forename: Ramana
authors.initials: R
authors.lastname: Madupu
authors.forename: William C
authors.initials: WC
authors.lastname: Nelson
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Dodson
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Brinkac
authors.forename: Sean C
authors.initials: SC
authors.lastname: Daugherty
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Durkin
authors.forename: Robert T
authors.initials: RT
authors.lastname: DeBoy
authors.forename: James F
authors.initials: JF
authors.lastname: Kolonay
authors.forename: Steven A
authors.initials: SA
authors.lastname: Sullivan
authors.forename: Liwei
authors.initials: L
authors.lastname: Zhou
authors.forename: Tanja M
authors.initials: TM
authors.lastname: Davidsen
authors.forename: Martin
authors.initials: M
authors.lastname: Wu
authors.forename: Adrienne L
authors.initials: AL
authors.lastname: Huston
authors.forename: Matthew
authors.initials: M
authors.lastname: Lewis
authors.forename: Bruce
authors.initials: B
authors.lastname: Weaver
authors.forename: Janice F
authors.initials: JF
authors.lastname: Weidman
authors.forename: Hoda
authors.initials: H
authors.lastname: Khouri
authors.forename: Terry R
authors.initials: TR
authors.lastname: Utterback
authors.forename: Tamara V
authors.initials: TV
authors.lastname: Feldblyum
authors.forename: Claire M
authors.initials: CM
authors.lastname: Fraser
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D002244:Carbon / Q000378:metabolism; D003081:Cold Climate; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004734:Energy Metabolism; D020563:Gammaproteobacteria / Q000235:genetics* / Q000378:metabolism*; D016680:Genome, Bacterial; D023281:Genomics; D008386:Marine Biology; D008560:Membrane Fluidity; D008954:Models, Biological; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D040901:Proteomics; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D002244:Carbon; D009584:Nitrogen