title: An alternative method to prepare samples of the pathogenic yeast Cryptococcus neoformans for scanning electron microscopy analysis.
abstract: In this work, an alternative to conventional preparation procedures for scanning electron microscopy (SEM) analysis of Cryptococcus neoformans was performed. The cells were fixed directly in the agar culture. This method is simpler than others already reported and the morphology of the cells was well preserved.
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, 91501-970, Porto Alegre, RS, Brazil.
authors.forename: Josiane
authors.initials: J
authors.lastname: Faganello
authors.forename: Walquíria
authors.initials: W
authors.lastname: Arruda
authors.forename: Augusto
authors.initials: A
authors.lastname: Schrank
authors.forename: Marilene Henning
authors.initials: MH
authors.lastname: Vainstein
mesh_terms: D000362:Agar; D003455:Cryptococcus neoformans / Q000648:ultrastructure*; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D000362:Agar