title: Differential response of two cell lines sequentially irradiated with low X-ray doses.
abstract: An experiment was designed to compare the effect of repeated low doses of X-rays in two different cell lines: one transformed, epithelial like and aneuploid Chinese hamster ovary K-1 (CHO-K1); the other originated from a human primary culture, fibroblast, diploid and non-transformed, MRC-5. CHO and MRC-5 cells were cultured for 14 or eight passages, respectively. Irradiation was performed once per passage when cells were in the quiescent state (90 - 95% in G1/G0). Cells were exposed to 10.0 mSv X-ray doses. Ionizing radiation did not induce apoptosis or necrosis in the exposed CHO cell population. Significant increases of low-level damaged cells (degrees 1 and 2) were found for the 14 cycles of radiation when compared with controls, except for the first irradiation cycle. No significant increases in the frequency of cells with severe damage were observed. The frequency of MRC-5 cells with low-level damage increased significantly when compared with controls for radiation cycles seven and eight. Significant increases of apoptosis, necrosis and severe damage were found only for the highest dose. Transformed and non-transformed cell types responded differently to direct and indirect damage using low-dose repeat exposures to ionizing radiation. Though more investigation is needed to understand the mechanisms of radiation effects in chronic low-dose-exposed cell populations, cellular type should be taken into account in the design of in vitro experiments for understanding low-dose-irradiation effects.
authors.affiliation: CIGEBA (Centro de Investigaciones en Genética Básica y Aplicada), Facultad de Ciencas Veterinarias, Universidad Nacional de La Plata, Argentina.
authors.forename: A M
authors.initials: AM
authors.lastname: Güerci
authors.forename: F N
authors.initials: FN
authors.lastname: Dulout
authors.forename: C A
authors.initials: CA
authors.lastname: Grillo
authors.forename: A I
authors.initials: AI
authors.lastname: Seoane
mesh_terms: D000818:Animals; D017209:Apoptosis; D016466:CHO Cells / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D009336:Necrosis; D011832:Radiation Injuries