title: Development of a sensitive and specific solid phase extraction--gas chromatography-tandem mass spectrometry method for the determination of elenolic acid, hydroxytyrosol, and tyrosol in rat urine.
abstract: A novel gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed, using an ion trap mass spectrometer, for the simultaneous determination of olive oil bioactive components, elenolic acid, hydroxytyrosol, and tyrosol, in rat urine. Samples were analyzed by GC-MS/MS prior to and after enzymatic treatment. A solid phase extraction sample pretreatment step with greater than 80% analytical recoveries for all compounds was performed followed by a derivatization reaction prior to GC-MS/MS analysis. The calibration curves were linear for all compounds studied for a dynamic range between 1 and 500 ng. The limit of detection was in the mid picogram level for tyrosol and elenolic acid (300 pg) and in the low picogram level for hydroxytyrosol (2.5 pg). The method was applied to the analysis of rat urine samples after sustained oral intake of oleuropein or extra virgin olive oil as a diet supplement.
authors.affiliation: Department of Pharmacy, University of Patras, Panepistimiopolis, Rio 265 04, Greece.
authors.forename: Fotini N
authors.initials: FN
authors.lastname: Bazoti
authors.forename: Evangelos
authors.initials: E
authors.lastname: Gikas
authors.forename: Caroline
authors.initials: C
authors.lastname: Puel
authors.forename: Véronique
authors.initials: V
authors.lastname: Coxam
authors.forename: Anthony
authors.initials: A
authors.lastname: Tsarbopoulos
mesh_terms: D000818:Animals; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D013058:Mass Spectrometry; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000652:urine; D011714:Pyrans / Q000652:urine*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011714:Pyrans; C005975:3,4-dihydroxyphenylethanol; C011867:4-hydroxyphenylethanol; C000352:elenolic acid; D010626:Phenylethyl Alcohol