Weighing in on ubiquitin: the expanding role of mass-spectrometry-based proteomics.
Mass-spectrometry-based proteomics has become an essential tool for the qualitative and quantitative analysis of cellular systems. The biochemical complexity and functional diversity of the ubiquitin system are well suited to proteomic studies. This review summarizes advances involving the identification of ubiquitinated proteins, the elucidation of ubiquitin-modification sites and the determination of polyubiquitin chain linkages, as well as offering a perspective on the application of emerging technologies for mechanistic and functional studies of protein ubiquitination.
Kirkpatrick
Donald S
DS
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
Denison
Carilee
C
Gygi
Steven P
SP
D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008954:Models, Biological; D010446:Peptide Fragments / Q000032:analysis; D025821:Polyubiquitin / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000379:methods; D025801:Ubiquitin / Q000032:analysis* / Q000737:chemistry; D043743:Ubiquitin-Protein Ligase Complexes / Q000737:chemistry; D014452:Ubiquitins / Q000032:analysis / Q000737:chemistry
D010446:Peptide Fragments; D011506:Proteins; D025801:Ubiquitin; D014452:Ubiquitins; D025821:Polyubiquitin; D043743:Ubiquitin-Protein Ligase Complexes