title: Gymnemic acids inhibit rabbit glyceraldehyde-3-phosphate dehydrogenase and induce a smearing of its electrophoretic band and dephosphorylation.
abstract: Gymnemic acids (GA) inhibited rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity. Binding of GA to GAPDH was observed by surface plasmon resonance measurement. Incubation of GAPDH with GA induced a smearing of the GAPDH band in SDS-PAGE. The GA-induced smearing was diminished by prior incubation of GA with gamma-cyclodextrin or by GA treatment with NAD. GA treatment did not affect the electrophoretic mobility of glucose-6-phosphate isomerase and dehydrogenase. GA treatment diminished the GAPDH band detected by an antibody to phosphoserine, but did not affect the phosphoserine bands of glucose-6-phosphate isomerase and dehydrogenase. These results indicated that GA specifically induced dephosphorylation of GAPDH.
authors.affiliation: Graduate School of Agriculture, Kyoto Prefectural University, Kyoto 606-8522, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Izutani
authors.forename: Takuya
authors.initials: T
authors.lastname: Murai
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Imoto
authors.forename: Masatake
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Oda
authors.forename: Sumio
authors.initials: S
authors.lastname: Ishijima
mesh_terms: D000818:Animals; D004586:Electrophoresis; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000037:antagonists & inhibitors*; D010766:Phosphorylation / Q000187:drug effects; D011817:Rabbits; D012503:Saponins / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D012503:Saponins; D014315:Triterpenes; C007898:gymnemic acid; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)