Probing cytoskeleton modulation by optical biosensors.
This paper reported the use of resonant waveguide grating biosensors for studying the cytoskeleton structure in cells. This was achieved by measuring the changes in mass within the bottom portion of cells upon exposure to saponin in the absence and presence of cytoskeleton modulators. Treatment of Chinese hamster ovary cells with saponin led to a dose-dependent and dynamic mass changes. When a higher concentration of saponin (> 60 microg/ml) was used, a net loss in mass was observed. This is probably resulted from the diffusion of soluble intracellular materials away from the bottom portion of cells after pore formation in the cell plasma membranes by saponin. The pretreatment of cells with actin disruption agents, cytochalasin B and latrunculin A, led to significantly increased loss in cell mass induced by either 75 or 125 microg/ml saponin. These results suggested that optical biosensors provide an attractive means to study the cytoskeleton structure and screen modulators that affect the cytoskeleton structure.
Fang
Ye
Y
Biochemical Technologies, Science and Technology Division, Corning Incorporated, Sullivan Park, Corning, NY 14870, USA. fangy2@corning.com
Ferrie
Ann M
AM
Li
Guangshan
G
D000818:Animals; D015374:Biosensing Techniques; D016466:CHO Cells; D006224:Cricetinae; D003599:Cytoskeleton / Q000502:physiology*; D055095:Optics and Photonics; D012503:Saponins / Q000494:pharmacology
D012503:Saponins