Nonradioactive telomerase activity assay by microchip electrophoresis: privileges to the classical gel electrophoresis assay.
The present study accents on the privileges of microchip-based electrophoresis to the conventional gel electrophoresis in separation of telomerase repeat amplification protocol/polymerase chain reaction (PCR) ladder products obtained in telomerase-catalyzed reaction in cancer cells. We try to clarify the interpretation of the results obtained by both electrophoretic procedures and to avoid misinterpretation as a result of PCR-dependent artefacts.
Zhelev
Zhivko
Z
Single-Molecule Bioanalysis Laboratory (SMBL), National Institute for Advanced Industrial Science and Technology, AIST-Shikoku, Takamatsu, Japan.
Bakalova
Rumiana
R
Ewis
Ashraf
A
Ohba
Hideki
H
Ishikawa
Mitsuru
M
Baba
Yoshinobu
Y
D046209:Electrophoresis, Microchip; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D020014:K562 Cells; D016133:Polymerase Chain Reaction; D019098:Telomerase / Q000032:analysis / Q000378:metabolism*
D019098:Telomerase