title: In vitro evaluation of platelet reactivity toward annuloplasty devices treated with heparin coating: studies under flow conditions.
abstract: We have applied an in vitro perfusion model to explore the potential thrombogenicity of polyester annulolasty fabric used in valve repair and to investigate the possible thromboresistance characteristics conferred by a special heparin coating (Duraflotrade mark treatment). Samples of human blood from i) untreated or ii) heparin-coated extracorporeal circuits were recirculated through annular perfusion chambers containing a) untreated or b) treated annuloplasty cloth material. Perfusion experiments were performed at a shear rate of 600 s(-1) for 20 min. Platelet interaction with the material was morphometrically evaluated. In experiments performed with blood from untreated circuits and cloth material, the average cross-sectional area of platelet mass was 615 +/- 135 microm2. Treatment of cloth material with Duraflotrade mark statistically decreased the area of interacting platelets to 319 +/- 101 microm2 (*p < 0.05, n = 10). Blood samples from heparin-coated extracorporeal circuits showed a decrease of total area of platelets (308 +/- 58 microm2 vs 138 +/- 30 microm2, *p < 0.05, n = 9). The combined treatment of Duraflotrade mark in extracorporeal circuits and cloth material caused a more consistent reduction (p < 0.05). The in vitro perfusion experimental model was sensitive to evaluate the thrombogenic potential of Duraflotrade mark treatment. Our results indicate that the heparin coating of cloth material and extracorporeal circuits improves the biocompatibility of the original material and reduces the thrombogenic profile.
authors.affiliation: Servicio de Hemoterapia-Hemostasia, Hospital Clínic, Facultad de Medicina, Fundació Clinic, IDIBAPS, Barcelona, Spain. agalan@clinic.ub.es
authors.forename: R
authors.initials: R
authors.lastname: Tonda
authors.forename: A M
authors.initials: AM
authors.lastname: Galán
authors.forename: M
authors.initials: M
authors.lastname: Pino
authors.forename: M R
authors.initials: MR
authors.lastname: Hernández
authors.forename: C
authors.initials: C
authors.lastname: Ayats
authors.forename: J L
authors.initials: JL
authors.lastname: Pomar
authors.forename: A
authors.initials: A
authors.lastname: Ordinas
authors.forename: G
authors.initials: G
authors.lastname: Escolar
mesh_terms: D000368:Aged; D000925:Anticoagulants / Q000494:pharmacology; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D002315:Cardiopulmonary Bypass / Q000295:instrumentation* / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005260:Female; D006351:Heart Valves / Q000473:pathology*; D006487:Hemostasis; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D010477:Perfusion; D019736:Prostheses and Implants
chemical_list: D000925:Anticoagulants; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D006493:Heparin