Cytotoxic effect of 7beta-hydroxycholesterol on human NCI-H460 lung cancer cells.
The cytotoxic activity of 7beta-hydroxycholesterol (7beta-OHC) was evaluated on human NCI-H460 lung cancer cells. 7beta-OHC decreased clonogenic survival of NCI-H460 in a dose dependent pattern. 7beta-OHC induced apoptosis in NCI-H460, with the characteristic features like increase in sub-G(1) hypodiploid (apoptotic) cells, and apoptotic body formation, as evidenced by flow cytometry and fluorescence microscope, respectively. Apoptosis was also associated with loss of mitochondrial transmembrane potential, and the activation of caspases 9 and 3. 7beta-OHC resulted in generation of reactive oxygen species (ROS) during apoptosis. On the whole, the results indicated that 7beta-OHC inhibited the proliferation of NCI-H460 cells through apoptosis via caspase activation.
Kang
Kyoung Ah
KA
Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Korea.
Chae
Sungwook
S
Lee
Kyoung Hwa
KH
Park
Moon Taek
MT
Lee
Su Jae
SJ
Lee
Yun Sil
YS
Hyun
Jin Won
JW
D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000473:pathology*; D008564:Membrane Potentials; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D017382:Reactive Oxygen Species
D006888:Hydroxycholesterols; D017382:Reactive Oxygen Species; C011724:cholest-5-en-3 beta,7 alpha-diol