Molecular cloning, phylogenetic analysis, expressional profiling and in vitro studies of TINY2 from Arabidopsis thaliana.
A cDNA that was rapidly induced upon abscisic acid, cold, drought, mechanical wounding and to a lesser extent, by high salinity treatment, was isolated from Arabidopsis seedlings. It was classified as DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and was closely related to the TINY gene, we named it TINY2. Gel retardation assay revealed that TINY2 was able to form a specific complex with the previously characterized DRE element while showed only residual affinity to the GCC box. When fused to the GAL4 DNA-binding domain, either full-length or its C-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay while its N-terminus was completely inactive. Our data indicate that TINY2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream genes in response to environmental stress.
Wei
Gang
G
The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
Pan
Yi
Y
Lei
Juan
J
Zhu
Yu-Xian
YX
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010802:Phylogeny; D012097:Repressor Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C107465:ERF protein, human; D012097:Repressor Proteins; C505418:TINY2 protein, Arabidopsis; D014157:Transcription Factors; D000040:Abscisic Acid