title: Modified vaccinia Ankara expressing HIVA antigen stimulates HIV-1-specific CD8 T cells in ELISpot assays of HIV-1 exposed infants.
abstract: Recombinant modified vaccinia virus Ankara expressing HIV-1 antigens (MVA.HIVA) was used in ELISpot assays to monitor HIV-1-specific T cell responses in infants. Responses to MVA.HIVA and HIV-1 peptides were examined in 13 infected and 81 exposed uninfected infants in Nairobi, Kenya. Responses to MVA.HIVA (38%) and peptide stimulation (38%) were similar in frequency (p=1.0) and magnitude (mean 176 versus 385 HIVSFU/10(6), p=0.96) in HIV-1 infected infants. In exposed uninfected infants, MVA.HIVA detected more positive responses and higher magnitude responses as compared to peptide. MVA.HIVA ELISpot is a sensitive method for quantification of HIV-1-specific CD8+ T cell responses in HIV-1 exposed infants. These results demonstrate the relevance of HIV-1 clade A consensus-derived immunogen HIVA for the viruses currently circulating in Nairobi.
authors.affiliation: Department of Medical Microbiology, University of Nairobi, Kenya.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Slyker
authors.forename: Barbara L
authors.initials: BL
authors.lastname: Lohman
authors.forename: Dorothy A
authors.initials: DA
authors.lastname: Mbori-Ngacha
authors.forename: Marie
authors.initials: M
authors.lastname: Reilly
authors.forename: Edmund G-T
authors.initials: EG
authors.lastname: Wee
authors.forename: Tao
authors.initials: T
authors.lastname: Dong
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: McMichael
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Rowland-Jones
authors.forename: Tomas
authors.initials: T
authors.lastname: Hanke
authors.forename: Grace
authors.initials: G
authors.lastname: John-Stewart
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000517:prevention & control; D015497:HIV-1 / Q000737:chemistry / Q000145:classification / Q000276:immunology*; D006801:Humans; D007223:Infant; D008213:Lymphocyte Activation / Q000276:immunology*; D014611:Vaccination; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
chemical_list: D015488:HIV Antigens; D014765:Viral Vaccines