B-1 cells are pivotal for in vivo inflammatory giant cell formation.
The mechanisms that govern giant cell (GC) formation in inflammatory, neoplastic and physiologic conditions are far from being understood. Here, we demonstrate that B-1 cells are essential for foreign-body GC formation in the mouse. GCs were analysed on the surface of glass cover slips implanted into the subcutaneous tissue of the animals. It was demonstrated that GCs are almost absent on cover slips implanted into the subcutaneous tissue of BALB/c or CBA/N X-linked immunodeficient mice. As these animals do not have B-1 cells in the peritoneal cavity, they were reconstituted with B-1 cells obtained from cultures of adherent mouse peritoneal cells. Results showed that in B-1-reconstituted animals, the number of GCs on the implant surface surpassed the values obtained with preparations from wild animals. In animals selectively irradiated (pleural and peritoneal cavities) to deplete these cavities of B-1 cells, GCs were also not formed. Enriched suspensions of B-1 cells grown in culture were labelled with [(3)H]-tymidine and injected into the peritoneal cavity of naive mice before implantation of glass cover slips. After 4 days, about 17% of mononuclear cells had their nuclei labelled, and almost 70% of GCs had one or more of their nuclei labelled when analysed by histoautoradiographic technique. A few GCs expressed an immunoglobulin M when analysed by immunostaining and confocal microscopy. Overall, these data demonstrate that B-1 cells are pivotal in the mechanisms of foreign-body GC formation in the mouse.
Bogsan
Cristina Stewart Bittencourt
CS
Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo, Brazil.
Novaes e Brito
Ronni Rômulo
RR
Palos
Maiko da Cruz
Mda C
Mortara
Renato Arruda
RA
Almeida
Sandro Rogério
SR
Lopes
José Daniel
JD
Mariano
Mario
M
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D002478:Cells, Cultured; D005547:Foreign Bodies / Q000276:immunology; D008040:Genetic Linkage; D015726:Giant Cells / Q000276:immunology*; D007075:Immunoglobulin M / Q000032:analysis; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D007249:Inflammation / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D010529:Peritoneal Cavity / Q000473:pathology; D014960:X Chromosome
D007075:Immunoglobulin M