A novel HPLC procedure for detection and quantification of aminoacetone, a precursor of methylglyoxal, in biological samples.
Increase in methylglyoxal is thought to be involved in different pathological conditions. Deamination of aminoacetone by semicarbazide-sensitive amine oxidase (SSAO) leads to production of methylglyoxal. We have synthesized aminoacetone and developed a novel HPLC procedure for its quantitative determination. The urinary excretion of aminoacetone is approximately 20-30 microg/mouse/day, and the concentration is about 0.5 microg/g in mouse liver and small intestine. SSAO inhibitor increases aminoacetone levels in both tissues and urines. Results confirm that aminoacetone is an endogenous substrate for SSAO. However, data also indicate that deamination is not the only catabolic pathway for aminoacetone.
Kazachkov
Michael
M
Neuropsychiatry Research Unit, Department of Psychiatry, University of Saskatchewan, A114 Medical Research Building, 103 Wiggins Road, Saskatoon, Sask., Canada.
Yu
Peter H
PH
D000096:Acetone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D000498:Allyl Compounds / Q000494:pharmacology; D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors / Q000378:metabolism; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D000837:Anion Exchange Resins; D002082:Butylamines / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005449:Fluorenes / Q000737:chemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D011765:Pyruvaldehyde / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
D000498:Allyl Compounds; D000596:Amino Acids; D000837:Anion Exchange Resins; D002082:Butylamines; D004791:Enzyme Inhibitors; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D000096:Acetone; C058000:mofegiline; D011765:Pyruvaldehyde; D006631:Amine Oxidase (Copper-Containing); C006495:aminoacetone