title: Large scale real-time PCR analysis of mRNA abundance in rainbow trout eggs in relationship with egg quality and post-ovulatory ageing.
abstract: The mRNA levels of 39 target genes were monitored in unfertilized eggs of 14 rainbow trout sampled the day of ovulation and again 5, 14, and 21 days later. For all 56 collected egg batches, an egg sample was fertilized to estimate egg quality by monitoring embryonic development. Remaining eggs were used for RNA extraction and subsequent real-time PCR analysis. A significant drop of egg quality was observed when eggs were held in the body cavity for 14 or 21 days post-ovulation (dpo). During the same period, eight transcripts (nucleoplasmin or Npm2, ferritin H, tubulin beta, JNK1, cyclin A1, cyclin A2, cathepsin Z, and IGF2) exhibited a differential abundance at one or several collection time(s). Interestingly, we observed higher levels of cyclins A1 and A2 mRNAs in eggs taken 5 days post-ovulation than in eggs taken, from the same females, at the time of ovulation. In addition, seven transcripts exhibited a differential abundance between low quality and high quality eggs. Low quality eggs were characterized by lower levels of Npm2, tubulin beta, and IGF1 transcripts. In contrast, keratins 8 and 18, cathepsin Z, and prostaglandin synthase 2 were more abundant in low quality eggs than in high quality eggs. In this study, we have demonstrated differences in mRNA levels in the rainbow trout egg that are reflective of developmental competence differences induced by post-ovulatory ageing. The putative role of these transcripts in post-ovulatory ageing-induced egg quality defects is discussed with special attention for corresponding cellular functions.
authors.affiliation: Institut National de la Recherche Agronomique, SCRIBE, Campus de Beaulieu, Rennes Cedex, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Aegerter
authors.forename: Bernard
authors.initials: B
authors.lastname: Jalabert
authors.forename: Julien
authors.initials: J
authors.lastname: Bobe
mesh_terms: D000367:Age Factors; D000818:Animals; D017931:DNA Primers; D005434:Flow Cytometry; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000378:metabolism*; D010060:Ovulation / Q000378:metabolism*; D010063:Ovum / Q000166:cytology* / Q000378:metabolism*; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric
chemical_list: D017931:DNA Primers; D011506:Proteins; D012333:RNA, Messenger