Genetic analysis of Trichinella populations by 'cold' single-strand conformation polymorphism analysis.
A non-isotopic single-strand conformation polymorphism ('cold' SSCP) technique has been assessed for the analysis of sequence variability in the expansion segment 5 (ES5) of domain IV and the D3 domain of nuclear ribosomal DNA within and/or among isolates and individual muscle (first-stage) larvae representing all currently recognized species/genotypes of Trichinella. Data are consistent with the ability of cold SSCP to identify intra-specific as well as inter-specific variability among Trichinella genotypes. The cold SSCP approach should be applicable to a range of other genetic markers for comparative studies of Trichinella populations globally.
Gasser
Robin B
RB
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia. robinbg@unimelb.edu.au
Hu
Min
M
El-Osta
Youssef Abs
YA
Zarlenga
Dante S
DS
Pozio
Edoardo
E
D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D014234:Trichinella / Q000235:genetics*
D018509:DNA, Helminth; D012335:RNA, Ribosomal