Stationary-phase expression and aminoacylation of a transfer-RNA-like small RNA.
Genome-scale analyses have shown numerous functional duplications in the canonical translational machinery. One of the most striking examples is the occurrence of unrelated class I and class II lysyl-transfer RNA synthetases (LysRS), which together may aminoacylate non-canonical tRNAs. We show that, in Bacillus cereus, the two LysRSs together aminoacylate a small RNA of unknown function named tRNA(Other), and that the aminoacylated product stably binds translation elongation factor Tu. In vitro reconstitution of a defined lysylation system showed that Lys-tRNA(Other) is synthesized in the presence of both LysRSs, but not by either alone. In vivo analyses showed that the class 2 LysRS was present both during and after exponential growth, whereas the class I enzyme and tRNA(Other) were predominantly produced during the stationary phase. Aminoacylation of tRNA(Other) was also found to be confined to the stationary phase, which suggests a role for this non-canonical tRNA in growth-phase-specific protein synthesis.
Ataide
Sandro F
SF
Department of Microbiology, The Ohio State University, Columbus, 43210-1292, USA.
Jester
Brian C
BC
Devine
Kevin M
KM
Ibba
Michael
M
D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry; D049148:Aminoacylation; D001409:Bacillus cereus / Q000201:enzymology* / Q000235:genetics*; D001412:Bacillus subtilis / Q000378:metabolism; D018407:Bacterial Physiological Phenomena; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D016678:Genome; D016680:Genome, Bacterial; D015151:Immunoblotting; D008239:Lysine / Q000737:chemistry; D010444:Peptide Elongation Factor Tu / Q000378:metabolism; D017433:Protein Structure, Secondary; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D012313:RNA; D012343:RNA, Transfer; D010444:Peptide Elongation Factor Tu; D000604:Amino Acyl-tRNA Synthetases; D008239:Lysine