title: Surface plasmon resonance for real-time evaluation of immobilized fructosyltransferase activity.
abstract: The extracellular enzyme fructosyltransferase (FTF) is considered to be a significant virulence factor in the dental biofilm. We have developed a method using surface plasmon resonance to detect the activity of immobilized FTF in situ. This real time technique provides a sensitive direct assay for characterizing functional properties of immobilized enzymes such as FTF.
authors.affiliation: Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah POB 12272, Jerusalem 91120, Israel.
authors.forename: Moshe
authors.initials: M
authors.lastname: Shemesh
authors.forename: Doron
authors.initials: D
authors.lastname: Steinberg
mesh_terms: D018441:Biofilms; D003773:Dental Plaque / Q000382:microbiology; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005630:Fructans / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D013295:Streptococcus mutans / Q000201:enzymology / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*; D001426:Bacterial Proteins
chemical_list: D004800:Enzymes, Immobilized; D005630:Fructans; D005632:Fructose; D005947:Glucose; D006602:Hexosyltransferases; C021451:inulosucrase; D001426:Bacterial Proteins