title: Detection of mercaptopyridines and mercaptopyrimidines in planar chromatography with iodine-azide reaction as a detection system.
abstract: Reaction between iodine and azide ion induced by mercaptopyridines and mercaptopyrimidines was utilized as a detection system in TLC and HPTLC. The developed plates were sprayed with a freshly prepared mixtures of sodium azide and starch solution adjusted to pH 5.5, and exposed to iodine vapour. The spots became visible as white spots on violet-grey background. The iodine-azide detection system has been proved to be the most favourable and enabled to detect quantities per spot in the range of 1-20 pmol (HPTLC) and 1-60 pmol (TLC). The iodine-azide tests were compared with other visualizing techniques commonly used in planar chromatography (iodine vapour and UV254). The developed method was applied to detection of thiopental in biological samples.
authors.affiliation: Department of Instrumental Analysis, University of Łódź, Pomorska 163, 90-236 Łódź, Poland. robzak@chemul.uni.lodz.pl
authors.forename: Robert
authors.initials: R
authors.lastname: Zakrzewski
authors.forename: Witold
authors.initials: W
authors.lastname: Ciesielski
mesh_terms: D001386:Azides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D007455:Iodine / Q000737:chemistry; D008875:Middle Aged; D011725:Pyridines / Q000032:analysis* / Q000097:blood / Q000652:urine; D011743:Pyrimidines / Q000032:analysis* / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013874:Thiopental / Q000097:blood / Q000652:urine
chemical_list: D001386:Azides; D011725:Pyridines; D011743:Pyrimidines; D013438:Sulfhydryl Compounds; C042861:4-thiopyridine; D007455:Iodine; D013874:Thiopental