Detection of herbicide subclasses by an optical multibiosensor based on an array of photosystem II mutants.
Massive use of herbicides in agriculture over the last few decades has become a serious environmental problem. The residual concentration of these compounds frequently exceeds the maximum admissible concentration in drinking water for human consumption and is a real environmental risk for the aquatic ecosystem. Herbicides inhibiting photosynthesis via targeting photosystem II function still represent the basic means of weed control. A multibiosensor was constructed for detecting herbicides using as biosensing elements photosynthetic preparations coupled to an optical fluorescence transduction system (Giardi et al. EU patent EP1134585, 01830148.1-2204); this paper is about its application in the detection of herbicide subclasses in river water. Photosynthetic material was immobilized on a silicio septum inside a series of flow cells, close to diodes so as to activate photosystem II (PSII) fluorescence. The principle of the detection was based on the factthat herbicides selectively modify PSII fluorescence activity. The multibiosensor has the original feature of being able to distinguish the subclasses of the photosynthetic herbicides by using specific immobilized biomediators isolated from mutated organisms. This setup resulted in a reusable, portable multibiosensor for the detection of herbicide subclasses with a half-life of 54 h for spinach thylakoids and limit of detection of 3 x 10(-9) M for herbicides present in river water.
Giardi
Maria Teresa
MT
Institute of Crystallography, Department of Molecular Design and Nanotechnology, CNR, Area of Research of Rome, Via Salaria Km 29, 3-Monterotondo Scalo, Rome, Italy. giardi@mlib.cnr.it
Guzzella
Licia
L
Euzet
Pierre
P
Rouillon
Regis
R
Esposito
Dania
D
D015374:Biosensing Techniques; D004784:Environmental Monitoring / Q000379:methods; D006540:Herbicides / Q000032:analysis* / Q000737:chemistry*; D009154:Mutation; D055095:Optics and Photonics; D010788:Photosynthesis / Q000187:drug effects; D045332:Photosystem II Protein Complex / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis*
D006540:Herbicides; D045332:Photosystem II Protein Complex; D012989:Soil Pollutants