Crohn's disease in Japanese is associated with a SNP-haplotype of N-acetyltransferase 2 gene.
OBJECTIVE
To investigate the frequency and distribution of N-acetyltransferase 2 (NAT2) and uridine 5'-diphosphate (UDP)-glucuronosyltransferase 1A7 (UGT1A7) genes in patients with ulcerative colitis (UC) and Crohn's disease (CD).


METHODS
Frequencies and distributions of NAT2 and UGT1A7 SNPs as well as their haplotypes were investigated in 95 patients with UC, 60 patients with CD, and 200 gender-matched, unrelated, healthy, control volunteers by PCR-restriction fragment length polymorphism (RFLP), PCR-denaturing high-performance liquid chromatography (DHPLC), and direct DNA sequencing.


RESULTS
Multiple logistic regression analysis revealed that the frequency of haplotype, NAT2*7B, significantly increased in CD patients, compared to that in controls (P = 0.0130, OR = 2.802, 95%CI = 1.243-6.316). However, there was no association between NAT2 haplotypes and UC, or between any UGT1A7 haplotypes and inflammatory bowel disease (IBD).


CONCLUSIONS
It is likely that the NAT2 gene is one of the determinants for CD in Japanese. Alternatively, a new CD determinant may exist in the 8p22 region, where NAT2 is located.
Machida
Haruhisa
H
Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
Tsukamoto
Kazuhiro
K
Wen
Chun-Yang
CY
Shikuwa
Saburou
S
Isomoto
Hajime
H
Mizuta
Yohei
Y
Takeshima
Fuminao
F
Murase
Kunihiko
K
Matsumoto
Naomichi
N
Murata
Ikuo
I
Kohno
Shigeru
S
Wen
Chen-Yang
CY
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D003093:Colitis, Ulcerative / Q000201:enzymology*; D003424:Crohn Disease / Q000201:enzymology* / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012016:Reference Values
D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human