Chemical reaction interface mass spectrometry with high efficiency nebulization.
A high efficiency nebulizer (HEN) coupled to a heated spray chamber and a membrane desolvator is used for liquid sample introduction in chemical reaction interface mass spectrometry (CRIMS). Compared to the conventional thermospray nebulizer operated at solvent flow rate of 1 mL/min, the HEN provides small droplets at lower flow rates (10-100 microL/min), improving the desolvation and analyte transport efficiency. As a result, the sensitivity for carbon detection by CRIMS is improved by a factor of 4. The new arrangement offers an easy-to-use and robust interface, facilitating the availability of a variety of liquid chromatographic techniques to the CRIMS. Separation and detection of labeled peptides in a mixture of unlabeled biopolymers is illustrated at a solvent flow rate of 45 microL/min as an example of new possibilities offered by the improved liquid introduction interface.
Jorabchi
Kaveh
K
Department of Chemistry, George Washington University, Washington, D.C. 20052, USA.
Kahen
Kaveh
K
Lecchi
Paolo
P
Montaser
Akbar
A
D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D014835:Volatilization
D010455:Peptides; D011506:Proteins