Using the chorions of fertilized zebrafish eggs as a biomaterial for the attachment and differentiation of mouse stem cells.
The development of proper biomaterials is critical for the success of cell therapy and modern tissue engineering. Here, we extruded the yolk and remaining inner mass from fertilized zebrafish eggs and used the resulting chorions as a biomaterial for the differentiation and attachment of mouse P19 embryonic carcinoma (EC) cells. Cells inserted into the chorion showed the spontaneous formation of embryoid body due to the repulsive cell adhesion of the chorion and differentiated specifically into neural cells and cardiomyocytes. In contrast, dissolved chorion extracellular matrix (ECM) conferred enhanced cell attachment on it, suggesting that a unique property of the zebrafish chorion with nanoporous structure appears to be responsible for the simple and controllable embryoid formation for stem cell differentiation. These results indicate that chorions from fertilized zebrafish eggs may be used as an extracellular matrix alternative and applied for stem cell differentiation to specific cell lineages.
Lee
Jin Woo
JW
Microsystem Research Center, Korea Institute of Science Technology, Seoul 136-791, Korea. bkju@korea.ac.kr
Na
Dae Seok
DS
Chae
Su Kyoung
SK
Kim
Choong
C
Kang
Ji Yoon
JY
Ju
Byeong Kwon
BK
Lee
Hwang
H
Kim
Sun Uk
SU
Hwang
Chang Nam
CN
Lee
Sang Ho
SH
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D002823:Chorion / Q000737:chemistry*; D054278:Embryonal Carcinoma Stem Cells; D051379:Mice; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D015027:Zebrafish / Q000196:embryology
D001672:Biocompatible Materials