[Direct fluorescent and indirect immunofluorescent assay in buffy coat for candidemia diagnosis in pediatric patients: a comparative study].
The diagnosis of candidemia by blood culture has poor sensitivity; therefore, immunossupresed patients and those with risk factors may receive empirical antifungal therapy, wich is potentially toxic. Fluorescent tests have been developed to obtain an early and more sensitive diagnosis than blood culture. The aim of this study was to compare indirect immunofluorescence vs direct calcofluor white fluorescence in buffy coat for candidemia diagnosis. Sensitivity, specificity, predictive values, of positive and negative samples were 60%, 86%, 33%, 95% and 90%, 80%, 35%, 99%, for indirect immunofluorescence and calcofluor white, respectively.
Flores-Ruiz
Eric Moisés
EM
Servicio de Infectología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores Delegación Cuauhtémoc, México DF, CP 06780, México. drericflores@yahoo.com
Miranda-Novales
María Guadalupe
MG
Solórzano-Santos
Fortino
F
Sánchez-Huerta
Gustavo
G
Díaz-Ponce
Humberto
H
D000293:Adolescent; D001557:Benzenesulfonates / Q000032:analysis; D002177:Candidiasis / Q000097:blood / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000032:analysis; D016469:Fungemia / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D016867:Immunocompromised Host; D007223:Infant; D007231:Infant, Newborn; D007962:Leukocytes / Q000382:microbiology*; D008297:Male; D008856:Microscopy, Fluorescence; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D001557:Benzenesulfonates; D005456:Fluorescent Dyes; C007061:C.I. Fluorescent Brightening Agent 28