title: Using the chorions of fertilized zebrafish eggs as a biomaterial for the attachment and differentiation of mouse stem cells.
abstract: The development of proper biomaterials is critical for the success of cell therapy and modern tissue engineering. Here, we extruded the yolk and remaining inner mass from fertilized zebrafish eggs and used the resulting chorions as a biomaterial for the differentiation and attachment of mouse P19 embryonic carcinoma (EC) cells. Cells inserted into the chorion showed the spontaneous formation of embryoid body due to the repulsive cell adhesion of the chorion and differentiated specifically into neural cells and cardiomyocytes. In contrast, dissolved chorion extracellular matrix (ECM) conferred enhanced cell attachment on it, suggesting that a unique property of the zebrafish chorion with nanoporous structure appears to be responsible for the simple and controllable embryoid formation for stem cell differentiation. These results indicate that chorions from fertilized zebrafish eggs may be used as an extracellular matrix alternative and applied for stem cell differentiation to specific cell lineages.
authors.affiliation: Microsystem Research Center, Korea Institute of Science Technology, Seoul 136-791, Korea. bkju@korea.ac.kr
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Lee
authors.forename: Dae Seok
authors.initials: DS
authors.lastname: Na
authors.forename: Su Kyoung
authors.initials: SK
authors.lastname: Chae
authors.forename: Choong
authors.initials: C
authors.lastname: Kim
authors.forename: Ji Yoon
authors.initials: JY
authors.lastname: Kang
authors.forename: Byeong Kwon
authors.initials: BK
authors.lastname: Ju
authors.forename: Hwang
authors.initials: H
authors.lastname: Lee
authors.forename: Sun Uk
authors.initials: SU
authors.lastname: Kim
authors.forename: Chang Nam
authors.initials: CN
authors.lastname: Hwang
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D002823:Chorion / Q000737:chemistry*; D054278:Embryonal Carcinoma Stem Cells; D051379:Mice; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D015027:Zebrafish / Q000196:embryology
chemical_list: D001672:Biocompatible Materials