title: Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides.
abstract: Modified nucleosides can be prepared by microbial transglycosylation from cheaper nucleoside precursors using free or immobilised whole cells. An efficient screening method to find transglycosylation activity in microorganisms was developed for the synthesis of 6-modified purine nucleosides, such as 6-chloro-, 6-methoxy-, 6-iodo- and 6-mercaptopurine ribonucleoside. Out of 100 microorganisms screened, Bacillus stearothermophilus ATCC 12980 was the best for this purpose.
authors.affiliation: Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina.
authors.forename: J A
authors.initials: JA
authors.lastname: Trelles
authors.forename: A L
authors.initials: AL
authors.lastname: Valino
authors.forename: V
authors.initials: V
authors.lastname: Runza
authors.forename: E S
authors.initials: ES
authors.lastname: Lewkowicz
authors.forename: A M
authors.initials: AM
authors.lastname: Iribarren
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D002384:Catalysis; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000378:metabolism*; D006031:Glycosylation; D007218:Industrial Microbiology / Q000191:economics / Q000379:methods; D009705:Nucleosides / Q000378:metabolism; D011684:Purine Nucleosides / Q000096:biosynthesis*; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism
chemical_list: D009705:Nucleosides; D011684:Purine Nucleosides; D011683:Purine-Nucleoside Phosphorylase