title: Protection by recombinant viral proteins against a respiratory challenge with virulent avian metapneumovirus.
abstract: Protection by recombinant avian metapneumovirus (aMPV) N or M proteins against a respiratory challenge with virulent aMPV was examined. N, M or N+M proteins were administered intramuscularly (IM) with incomplete Freund's adjuvant (IFA) or by the oculonasal (ON) route with cholera toxin-B (CTB). Each turkey received 40 or 80 microg of each recombinant protein. Birds were considered protected against challenge if the challenge virus was not detectable in the choanal swabs by RT-PCR. At a dose of 40 microg/bird, N protein given with IFA by the IM route protected eight out of nine birds. M protein at the same dose protected three out of seven birds, while a combination of N+M proteins (40 microg each) protected three out of four birds. At a dose of 80 microg of each of N and M proteins per bird given with IFA by the IM route, 100% protection was achieved. ON immunization with a mixture of N and M proteins induced partial protection when the proteins were given with CTB; no detectable protection was noted without CTB. N and M proteins induced anti-aMPV antibodies, although protection against virulent virus challenge did not appear to be associated with the level or presence of antibodies.
authors.affiliation: Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
authors.forename: Parag
authors.initials: P
authors.lastname: Chary
authors.forename: M Kariuki
authors.initials: MK
authors.lastname: Njenga
authors.forename: Jagdev M
authors.initials: JM
authors.lastname: Sharma
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D029121:Metapneumovirus / Q000276:immunology* / Q000472:pathogenicity*; D018184:Paramyxoviridae Infections / Q000276:immunology* / Q000517:prevention & control* / Q000662:veterinary / Q000821:virology; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000276:immunology; D014422:Turkeys / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D011994:Recombinant Proteins; D014764:Viral Proteins; D014765:Viral Vaccines