title: [Oxidative damage and apoptosis in NIT-1 cells induced by ethanol].
abstract: OBJECTIVE
  To decide whether ethanol induce apoptosis in mouse insulinoma cells (NIT-1) and the potential mechanism involved.
  
  
  METHODS
  After NIT-1 cells were exposed to various concentrations of ethanol, agarose gel electrophoresis was used to detected DNA fragmentation. malondialdehyde (MDA) content, glutathione (GSH) level, superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px) were measured in NIT-1 cells to evaluate the oxidative damage degree. Caspase-3 relative activity was determined by colorimetric assay.
  
  
  RESULTS
  The results showed a "DNA ladder" pattern after treated with ethanol in NIT-1 cells. The MDA content was higher, the SOD activity, GSH-Px activity and GSH content were lower in ethanol group than those of control group. Ethanol increased the caspase-3 activity by a dose and time dependent manner.
  
  
  CONCLUSIONS
  Higher concentration of ethanol induces imbalance of oxidation and antioxidative ability in NIT-1 cells, and oxidative stress further leads to apoptosis which may be related to the increased caspase-3 activity.
authors.affiliation: School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Hao
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Hu
authors.forename: Xiu-Fa
authors.initials: XF
authors.lastname: Sun
authors.forename: Chen-Jiang
authors.initials: CJ
authors.lastname: Ying
authors.forename: Liegang
authors.initials: L
authors.lastname: Liu
authors.forename: Limei
authors.initials: L
authors.lastname: Mao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D000431:Ethanol / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007340:Insulinoma / Q000378:metabolism* / Q000473:pathology*; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000431:Ethanol; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C505409:Casp3 protein, mouse; D053148:Caspase 3; D005978:Glutathione