Gene silencing using small interference RNA in mast cells.
Small interfering RNA (siRNA) is a potent and specific method of inducing gene silencing through induction of RNA interference. siRNAs can be allowed for in vitro and in vivo applications. siRNAs have been successfully studied in vitro, but little is known about its efficacy in vivo. We have successfully applied the siRNA technique for silencing glucose transporter 3 in cultured L6 muscle cells. The siRNA technique has been used efficiently to silence RasGRP4 expression in mast cells.
Yin
Deling
D
Department of Internal Medicine, Quillen College of Medicine, East Tennessee State University, Johnson City, TN, USA.
Stuart
Charles A
CA
D002460:Cell Line; D020868:Gene Silencing; D051274:Glucose Transporter Type 3 / Q000235:genetics / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D020663:ras Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism
D051274:Glucose Transporter Type 3; C462507:RASGRP4 protein, human; D034741:RNA, Small Interfering; D020663:ras Guanine Nucleotide Exchange Factors