[Preliminary report: effects of artificial blood on hypoxic pulmonary vasoconstriction].
BACKGROUND
We examined the effects of artificial blood on hypoxic pulmonary vasoconstriction (HPV).


METHODS
Pump-perfused rabbit lungs were reperfused and ventilated with a mixture of 21% O2, 5% CO2, and balance N2. HPV was induced by reduction of O2 concentration from 21 to 3% for 5 min. Pulmonary arterial pressure (PAP) was measured using a low-pressure transducer. Two perfusion solutions, (1) a mixture of autologous red blood cells and physiological salt solution supplemented with 5% albumin and (2) artificial red blood cells (liposome-encapsulated hemoglobin) and physiological saline supplemented with 5% albumin, were made. Using each solution, HPV was evaluated by PAP increase with and without inhalation of nitric oxide (NO).


RESULTS
(1) With both autologous and artificial perfusion solutions, hypoxic stimulation (HS) increased PAP. (2) With the autologous solution, NO inhalation suppressed the HS-induced PAP increase, but with the artificial solutions no such a phenomenon was observed.


CONCLUSIONS
NO inhalation failed to suppress HPV with the artificial solution, probably since the liposome-encapsulated hemoglobin strongly inactivated NO.
Oshima
Yoshiaki
Y
Department of Anesthesiology, Shimane Prefectural Central Hospital, Izumo 693-8555.
Okazaki
Naoto
N
D000280:Administration, Inhalation; D000818:Animals; D001802:Blood Substitutes / Q000009:adverse effects*; D005260:Female; D000860:Hypoxia; D009569:Nitric Oxide / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010477:Perfusion; D011651:Pulmonary Artery / Q000187:drug effects*; D011669:Pulmonary Wedge Pressure / Q000187:drug effects; D011817:Rabbits; D014661:Vasoconstriction / Q000187:drug effects*
D001802:Blood Substitutes; D009569:Nitric Oxide