title: Cellular immune response induced by Salmonella enterica serotype Typhi iron-regulated outer-membrane proteins at peripheral and mucosal levels.
abstract: The role of purified iron-regulated outer-membrane proteins (IROMPs) from Salmonella enterica serotype Typhi in modulation of specific T-cell responses was studied. The cellular immune response induced by IROMPs was measured by assessing the delayed-type hypersensitivity (DTH) response, lymphocyte proliferation, T-cell phenotyping and cytokine-producing cells using lymphocytes isolated from the spleen and Peyer's patches of IROMPs-immunized, immunized-challenged, infected and control mice. IROMPs immunization resulted in an enhanced DTH response and exhibited a significant increase in the protein-specific proliferative response of lymphocyte from the spleen as well as Peyer's patches. A significant increase was also observed in the ratio of CD4+/CD8+ cells in the immunized mice as compared to the infected mice. Results of the cytokine analysis revealed that during the initial period there was increased production of interleukin (IL)-2- and interferon (IFN)-gamma-producing cells in the spleen and Peyer's patches, indicating a Th1 type response, whereas in the later period of the study, increased production of IL-4-producing cells suggested a Th2 type response. The results of this study suggest a role for S. Typhi IROMPs in modulating the cellular immune response at peripheral and mucosal levels.
authors.affiliation: Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India 2Department of Microbiology, Panjab University, Chandigarh 160 014, India.
authors.forename: Shaloo
authors.initials: S
authors.lastname: Sood
authors.affiliation: Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India 2Department of Microbiology, Panjab University, Chandigarh 160 014, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Rishi
authors.affiliation: Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India 2Department of Microbiology, Panjab University, Chandigarh 160 014, India.
authors.forename: Harpreet
authors.initials: H
authors.lastname: Vohra
authors.affiliation: Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India 2Department of Microbiology, Panjab University, Chandigarh 160 014, India.
authors.forename: Saroj
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India 2Department of Microbiology, Panjab University, Chandigarh 160 014, India.
authors.forename: Nirmal K
authors.initials: NK
authors.lastname: Ganguly
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage / Q000276:immunology*; D016207:Cytokines; D005260:Female; D006968:Hypersensitivity, Delayed; D007114:Immunization; D016130:Immunophenotyping; D007501:Iron / Q000378:metabolism*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology; D012485:Salmonella typhi / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D016207:Cytokines; D007501:Iron