title: CD98, a novel marker of transient amplifying human keratinocytes.
abstract: Identification of plasma membrane markers of basal keratinocytes is essential for sorting basal cells and, subsequently, adult epidermal stem cells. In this study, we isolated caveolin-1-enriched microdomains from human HaCaT keratinocytes and identified proteins representing potential cell surface markers of the epidermis by a proteomic approach. The purification of this caveolae domain allowed us to characterize 53 proteins of which 26% were transmembrane and 32% associated-membrane proteins. One of them, CD98, was found to be co-localized with beta1 integrin at the plasma membrane of the basal keratinocytes of healthy human epidermis. We then isolated CD98-positive keratinocytes from fresh skin biopsies. Using clonogenic assays, we demonstrate that CD98 may be considered as a marker of transient amplifying human keratinocytes.
authors.affiliation: Service de Génomique Fonctionnelle, CEA, Evry, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Lemaître
authors.forename: Florence
authors.initials: F
authors.lastname: Gonnet
authors.forename: Pierre
authors.initials: P
authors.lastname: Vaigot
authors.forename: Xavier
authors.initials: X
authors.lastname: Gidrol
authors.forename: Michèle T
authors.initials: MT
authors.lastname: Martin
authors.forename: Jeanine
authors.initials: J
authors.lastname: Tortajada
authors.forename: Gilles
authors.initials: G
authors.lastname: Waksman
mesh_terms: D051242:Caveolin 1 / Q000378:metabolism; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D027261:Fusion Regulatory Protein-1 / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D015603:Keratinocytes / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D051242:Caveolin 1; D027261:Fusion Regulatory Protein-1