Mitochondrial gene knockout HL60rho0 cells show preferential differentiation into monocytes/macrophages.
This study compares the differentiation potential of mitochondrial gene knockout (rho0) and parental HL60 cells in response to 1.25% dimethylsulfoxide (DMSO) and 10nM phorbol myristate acetate (PMA). Compared to HL60 cells, undifferentiated HL60rho0 cells showed partial monocyte/macrophage differentiation, with increased CD11c and CD14 expression, decreased CD71 expression, and weak non-specific esterase staining. Differentiation along the monocyte/macrophage pathway (PMA) was more pronounced in HL60rho0 than parental HL60 cells with increased CD11c and CD14 expression and stronger non-specific esterase staining. DMSO-exposure resulted in a poorly differentiated nuclear morphology, small respiratory burst and marginal up-regulation of CD15 expression in HL60rho0 cells.
Herst
Patries M
PM
Malaghan Institute of Medical Research, P.O. Box 7060, Wellington, New Zealand.
Levine
D Matt
DM
Berridge
Michael V
MV
D015703:Antigens, CD / Q000378:metabolism; D039521:CD11c Antigen / Q000378:metabolism; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004579:Electron Transport; D016166:Free Radical Scavengers / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D016256:Lewis X Antigen / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008264:Macrophages / Q000166:cytology*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology*; D010101:Oxygen Consumption; D010587:Phagocytosis; D011990:Receptors, Transferrin / Q000378:metabolism; D016897:Respiratory Burst / Q000502:physiology
D015703:Antigens, CD; D039521:CD11c Antigen; C068126:CD71 antigen; D004272:DNA, Mitochondrial; D016166:Free Radical Scavengers; D016256:Lewis X Antigen; D018950:Lipopolysaccharide Receptors; D011990:Receptors, Transferrin; D004121:Dimethyl Sulfoxide