title: Development of a micro dual beam fluorometric detector specific for microchip analysis of benzo[a]pyrene and benzo[k]fluoranthene in diesel exhaust particulate samples.
abstract: We developed a device and some systems for detecting benzo[a]pyrene (B[a]P) and benzo[k]fluoranthene (B[k]F). The device uses a UV light-emitting diode that emits light with a wavelength of 370 nm and a violet laser diode that emits light with a wavelength of 395 nm as excitation light sources. The detection method is based on the following observation: characteristic fluorescence is emitted from both B[a]P and B[k]F, with intensities up to 10 times greater than those from 22 other polycyclic aromatic hydrocarbons (PAHs). The excitation and emission wavelengths for fluorescence from B[a]P and B[k]F are 370 and 429 nm, and 395 and 429 nm, respectively. Further, we calculated their concentrations in diesel exhaust particles by means of two calibration curves determined using a mixture of 24 PAHs. The detection limits of the proposed device with a microchip are 5.58 microg L(-1) for B[a]P and 6.03 microg L(-1) for B[k]F. We applied this method to analyze B[a]P or B[k]F contained in three types of diesel exhaust particles, and the results agreed well with those obtained by liquid chromatography.
authors.affiliation: Department of Chemistry, Graduated School of Science, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji Tokyo 192-0397, Japan. add9code@comp.metro-u.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Masaki
authors.forename: Hironori
authors.initials: H
authors.lastname: Susaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Korenaga
mesh_terms: D001564:Benzo(a)pyrene / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods*; D004867:Equipment Design; D005449:Fluorenes / Q000032:analysis; D005470:Fluorometry / Q000295:instrumentation; D006801:Humans; D056656:Lab-On-A-Chip Devices; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D001335:Vehicle Emissions / Q000032:analysis*
chemical_list: D005449:Fluorenes; D011084:Polycyclic Aromatic Hydrocarbons; D001335:Vehicle Emissions; D001564:Benzo(a)pyrene; C022921:benzo(k)fluoranthene