title: Peptide hormones and histamine in plasma and synovial fluid of patients with rheumatoid arthritis and osteoarthrosis.
abstract: OBJECTIVE
  Hormones other than adrenal and gonadal steroids may play also a significant role in the pathogenesis of rheumatoid arthritis. The aim of this study was to investigate the levels of selected peptide hormones and histamine in synovial fluid of knee joints and in plasma of patients with rheumatoid arthritis and with osteoarthrosis.
  
  
  METHODS
  The concentrations of insulin, C-peptide, prolactin, growth hormone, free triiodothyronine (FT3), thyrotropin (TSH), and histamine were determined in synovial fluid and plasma of 27 patients with rheumatoid arthritis (RA) and in 12 patients with osteoarthrosis (OA).
  
  
  RESULTS
  The presence of peptide hormones in synovial fluid was demonstrated. The levels of TSH and growth hormone were lower in synovial fluid than in plasma in both groups, while those of prolactin were comparable in synovial fluid and in plasma. The levels of C-peptide (p < 0.05), insulin and FT3 were higher in synovial fluid than in plasma of OA patients, but lower in synovial fluid of RA patients as compared to their levels in plasma. Significant positive correlations between the levels in plasma and synovial fluid were observed in prolactin (p < 0.001, r = 0.741) and TSH (p < 0.05, r = 0.88) only. After age adjustment, no significant differences in synovial fluid and in plasma levels of all hormones were found between OA and RA patients. The levels of histamine in plasma were similar in RA and OA patients, in synovial fluid of both groups histamine was found in almost undetectable amounts.
  
  
  CONCLUSIONS
  The selected peptide hormones, e.g. insulin, C-peptide, prolactin, growth hormone, FT3 and TSH, are present in synovial fluid of RA and OA patients, some of them in the concentrations comparable to these in plasma. The role of the locally present hormones in pathogenesis of RA has to be investigated in further studies and analyses.
authors.affiliation: National Institute for Rheumatic Diseases, Piestany, Slovakia.
authors.forename: J
authors.initials: J
authors.lastname: Rovensky
authors.forename: R
authors.initials: R
authors.lastname: Imrich
authors.forename: Z
authors.initials: Z
authors.lastname: Radikova
authors.forename: E
authors.initials: E
authors.lastname: Simorova
authors.forename: O
authors.initials: O
authors.lastname: Greguska
authors.forename: M
authors.initials: M
authors.lastname: Vigas
authors.forename: L
authors.initials: L
authors.lastname: Macho
mesh_terms: D001172:Arthritis, Rheumatoid / Q000097:blood / Q000378:metabolism*; D002096:C-Peptide / Q000032:analysis / Q000097:blood; D005260:Female; D006632:Histamine / Q000032:analysis* / Q000097:blood; D019382:Human Growth Hormone / Q000032:analysis / Q000097:blood; D006801:Humans; D007328:Insulin / Q000032:analysis / Q000097:blood; D007719:Knee Joint; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000097:blood / Q000378:metabolism*; D036361:Peptide Hormones / Q000032:analysis* / Q000097:blood; D011388:Prolactin / Q000032:analysis / Q000097:blood; D013582:Synovial Fluid / Q000737:chemistry*; D013972:Thyrotropin / Q000032:analysis / Q000097:blood; D014284:Triiodothyronine / Q000032:analysis / Q000097:blood
chemical_list: D002096:C-Peptide; D007328:Insulin; D036361:Peptide Hormones; D014284:Triiodothyronine; D019382:Human Growth Hormone; D006632:Histamine; D011388:Prolactin; D013972:Thyrotropin