title: Heat stable ssDNA/RNA-binding activity of a wheat cold shock domain protein.
abstract: The cold-induced wheat WCSP1 protein belongs to the cold shock domain (CSD) protein family. In prokaryotes and eukaryotes, the CSD functions as a nucleic acid-binding domain. Here, we demonstrated that purified recombinant WCSP1 is boiling soluble and binds ss/dsDNA and mRNA. Furthermore, boiled-WCSP1 retained its characteristic nucleic acid-binding activity. A WCSP1 deletion mutant, containing only a CSD, lost ssDNA/RNA-binding activity; while a mutant containing the CSD and the first glycine-rich region (GR) displayed the activity. These data indicated that the first GR of WCSP1 is necessary for the binding activity but is not for the heat stability of the protein.
authors.affiliation: Winter Stress Laboratory, Department of Low Temperature Sciences, National Agricultural Research Center for Hokkaido Region, NARO, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nakaminami
authors.forename: Kentaro
authors.initials: K
authors.lastname: Sasaki
authors.forename: Shinya
authors.initials: S
authors.lastname: Kajita
authors.forename: Hisashi
authors.initials: H
authors.lastname: Takeda
authors.forename: Dale
authors.initials: D
authors.lastname: Karlson
authors.forename: Kazuko
authors.initials: K
authors.lastname: Ohgi
authors.forename: Ryozo
authors.initials: R
authors.lastname: Imai
mesh_terms: D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D014908:Triticum; D016335:Zinc Fingers
chemical_list: D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D005998:Glycine