title: Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA).
abstract: Cell/tissue-specific gene expression are tightly regulated by various combinations of multiple transcription factors (TFs). Here, we present an oligonucleotide array-based transcription factor assay (OATFA), which allows the simultaneous assay of multiple active TFs. In this proof-of-principle work, both purified TFs and cell extracts were analyzed using OATFA and further antibody-based validation confirmed the chip data. This method could simplify the assay of multiple TFs and may facilitate high-throughput profiling of large numbers of TFs.
authors.affiliation: Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shao
authors.forename: Hua-Jiang
authors.initials: HJ
authors.lastname: Wei
authors.forename: Ji-Ying
authors.initials: JY
authors.lastname: Qiao
authors.forename: Yong-Chao
authors.initials: YC
authors.lastname: Zhao
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Sun
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
mesh_terms: D001681:Biological Assay / Q000379:methods; D002467:Cell Nucleus / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D014157:Transcription Factors / Q000032:analysis*
chemical_list: D014157:Transcription Factors