title: Cardiopulmonary bypass reduces atrial Na+-K+-ATPase expression in children.
abstract: Cardiopulmonary bypass (CPB) may induce serious side effects, potentially leading to myocardial failure. The Na(+)-K(+)-ATPase is a key component for myocardial function. Due to its developmental regulation, results from adult studies cannot be adopted to the situation in childhood. Right atrial myocardium from patients with left-to-right shunts at atrial level (VO, n=8) and those without (NO, n=8) was excised during heart surgery before and after CPB. Na(+)-K(+)-ATPase isoforms ATP1A1 (p=0.008) and ATP1A3 (p=0.038) decreased during CPB, which decrease was restricted to the VO group. This study highlights the importance of the underlying heart defect for susceptibility to the effects of CPB, showing a reduced Na(+)-K(+)-ATPase mRNA expression only in patients with left-to-right shunts on the atrial level. This seemed to be an early molecular event, as apart from one, none of the patients showed heart failure before or after surgery.
authors.affiliation: Division of Pediatric Cardiology, Department of Pediatrics, University Hospital, 3010 Berne, Switzerland. mladen.pavlovic@insel.ch
authors.forename: Mladen
authors.initials: M
authors.lastname: Pavlovic
authors.forename: André
authors.initials: A
authors.lastname: Schaller
authors.forename: Roland A
authors.initials: RA
authors.lastname: Ammann
authors.forename: Javier
authors.initials: J
authors.lastname: Sanz
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Pfammatter
authors.forename: Thierry
authors.initials: T
authors.lastname: Carrel
authors.forename: Pascal
authors.initials: P
authors.lastname: Berdat
authors.forename: Sabina
authors.initials: S
authors.lastname: Gallati
mesh_terms: D000293:Adolescent; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D001026:Coronary Artery Bypass; D017931:DNA Primers; D005260:Female; D006325:Heart Atria / Q000201:enzymology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase