title: [Study on low-temperature alkaline lipase from Bohaisea-9145].
abstract: Lipase-overproducing marine yeast Bohaisea-9145 isolated from the Bohai sea region was identified as psychrotrophlic Yarrowia lipolytica. The optimum composition of culture medium was ground soybean 4%, rice bran 4%, peanut power 4%, crude peanut oil 0.5%, MgSO4 0.05%, KH2PPO4 0.2%. The optimum temperature and pH were 26 +/- 1 degrees C pH5.0, the time of fermentation cycle was 23h. The lipase activity was improved to 258.67 U/mL through optimization, four-fold more than that of initial strain. The lipase displayed maximum activity at pH 8.5, 35 degrees C and was stable within the range of pH 4.0 - 9.0 at low temperature. It had high thermosensitivity, good compatibility with familiar metal ions and chemical reagents. It was antioxidant and had strong resistance to high salinity. As a novel marine low-temperature lipase, it has promising prospect in many fields, especially as an additive of detergent.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. stjouqd-2001@sohu.com
authors.forename: Tie-Juan
authors.initials: TJ
authors.lastname: Shao
authors.forename: Mi
authors.initials: M
authors.lastname: Sun
authors.forename: Jia-Sheng
authors.initials: JS
authors.lastname: Zheng
authors.forename: Yue-Jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Xiu-Bao
authors.initials: XB
authors.lastname: Qiu
mesh_terms: D008049:Lipase / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013696:Temperature; D014871:Water Microbiology; D025062:Yarrowia / Q000201:enzymology* / Q000235:genetics
chemical_list: D008049:Lipase