Green tea polyphenol extract attenuates colon injury induced by experimental colitis.
Inflammatory bowel disease (IBD) is characterised by oxidative and nitrosative stress, leukocyte infiltration, and up-regulation of intercellular adhesion molecule 1 (ICAM-1) expression in the colon. The aim of the present study was to examine the effects of green tea extract in rats subjected to experimental colitis induced by intracolonic instillation of dinitrobenzene sulphonic acid (DNBS). At 4 days after DNBS administration the rats were sacrificed. Treatment with green tea extract significantly attenuated diarrhoea and loss of body weight. This was associated with a remarkable amelioration of the disruption of the colonic architecture, significant reduction of colonic myeloperoxidase (MPO) and tumor necrosis factor-alpha (TNF-alpha) production. Green tea extract also reduced the appearance of nitrotyrosine immunoreactivity in the colon and reduced the up-regulation of ICAM-1.
Mazzon
Emanuela
E
Department of Clinical and Experimental Medicine and Pharmacology, Torre Biologica, Policlinico Universitario, Messina, Italy.
Muià
Carmelo
C
Paola
Rosanna Di
RD
Genovese
Tiziana
T
Menegazzi
Marta
M
De Sarro
Angelina
A
Suzuki
Hisanori
H
Cuzzocrea
Salvatore
S
D000818:Animals; D001557:Benzenesulfonates; D002851:Chromatography, High Pressure Liquid; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology / Q000517:prevention & control; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use*; D006419:Heme Oxygenase (Decyclizing) / Q000032:analysis; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use*; D008517:Phytotherapy; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
D001557:Benzenesulfonates; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013662:Tea; D014409:Tumor Necrosis Factor-alpha; C007488:dinitrobenzenesulfonic acid; D018799:Intercellular Adhesion Molecule-1; C002744:3-nitrotyrosine; D014443:Tyrosine; D009195:Peroxidase; D006419:Heme Oxygenase (Decyclizing)