Polyclonal antibodies inhibit the glycation-induced inactivation of bovine Cu,Zn-superoxide dismutase.
In vitro incubation of bovine Cu,Zn-SOD (Cu,Zn-superoxide dismutase) with glucose, ribose or fructose results in a remarkable inactivation of the enzyme. There was a progressive decrease in enzyme activity on incubation with glucose and, at the end of 7 days, only 26% of the initial activity remained. The inactivation was accompanied by a parallel decrease in the amount of protein detectable on gels after SDS/PAGE. Reaction of the sugars with SOD was ascertained using an immunoblot assay in which sugar-incubated SOD was derivatized with 2,4-dinitrophenylhydrazine and allowed to react with the dinitrophenol-specific antibody. Affinity purified antibodies from the sera of rabbits immunized with bovine SOD were highly effective in restricting the inactivation of the enzyme induced by glucose, ribose or fructose.
Jabeen
Rukhsana
R
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 2002 India.
Saleemuddin
M
M
D000818:Animals; D002417:Cattle; D004912:Erythrocytes / Q000201:enzymology; D016166:Free Radical Scavengers / Q000037:antagonists & inhibitors; D005632:Fructose / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D007074:Immunoglobulin G / Q000276:immunology*; D011506:Proteins / Q000032:analysis; D012266:Ribose / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors* / Q000276:immunology
D016166:Free Radical Scavengers; D007074:Immunoglobulin G; D011506:Proteins; D005632:Fructose; D012266:Ribose; D013482:Superoxide Dismutase; D005947:Glucose