title: Secreted autotransporter toxin produced by a diffusely adhering Escherichia coli strain causes intestinal damage in animal model assays.
abstract: Bacterial diarrheal diseases remain a major cause of morbidity and mortality in developing countries. Diffusely adhering Escherichia coli (DAEC) is a newly proposed category of diarrheagenic E. coli based on epidemiological studies. Sat, a new virulence factor of some uropathogeic Escherichia coli, was described with a vacuolating cytotoxic action in bladder and kidney tissues. In the present study, we analyzed the Sat effects, produced by a DAEC strain in rabbit ileal intestinal tissue and cultured epithelial cells. We observed enterotoxic activity in rabbit ileum tissues by Ussing chamber assays, a pronounced fluid accumulation in rabbit ileum loops with villous necrosis observed in the histopathologic examination, and morphological changes in monolayer cultures of Y1 adrenal cells. Our results suggest that DAEC strains may be involved in diarrhea.
authors.affiliation: Faculdade de Ciências Farmacêuticas, Departamento de Análises, Clinicas e Toxicologicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580 - bl. 17, CEP 05508-900, São Paulo, Brazil.
authors.forename: Carla R
authors.initials: CR
authors.lastname: Taddei
authors.forename: Alessio
authors.initials: A
authors.lastname: Fasano
authors.forename: Antônio J P
authors.initials: AJ
authors.lastname: Ferreira
authors.forename: Luiz R
authors.initials: LR
authors.lastname: Trabulsi
authors.forename: Marina B
authors.initials: MB
authors.lastname: Martinez
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001427:Bacterial Toxins / Q000633:toxicity*; D002460:Cell Line; D003967:Diarrhea / Q000382:microbiology* / Q000473:pathology; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000378:metabolism / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000473:pathology; D029968:Escherichia coli Proteins / Q000633:toxicity*; D007082:Ileum / Q000473:pathology*; D051379:Mice; D011817:Rabbits; D037521:Virulence Factors / Q000633:toxicity
chemical_list: D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; D037521:Virulence Factors; C506813:secreted autotransporter toxin, E coli