title: Simultaneous detection of double-stranded RNA-induced protein kinase and its specific mRNA by single cell analysis.
abstract: Fluorescent in situ hybridization was combined with flow cytometry to detect the expression of the double-stranded-RNA-induced protein kinase (PKR) in single cells. Labeled anti-sense oligonucleotide was used to target the specific mRNA while the protein was targeted with an antibody. It was demonstrated that the PKR-mRNA signal could be protected through a lengthy immunostaining procedure. The expression pattern of the PKR-mRNA with respect to DNA content was shown to be comparable to that of 18S ribosomal RNA.
authors.affiliation: Chemical Engineering Program, University of California La Jolla, San Diego, 92093, USA.
authors.forename: Mónica M
authors.initials: MM
authors.lastname: Lozano
authors.forename: Tanya L
authors.initials: TL
authors.lastname: Chantawansri
authors.forename: Pao C
authors.initials: PC
authors.lastname: Chau
mesh_terms: D045744:Cell Line, Tumor; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D005456:Fluorescent Dyes; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D011494:Protein Kinases