Deinococcus radiodurans strain R1 contains N6-methyladenine in its genome.
Methylation of DNA is known to be involved in DNA repair mechanisms in bacteria. Deinococcus radiodurans strain R1 on exposure to high radiation undergoes significant DNA damage, which is repaired without mutations. However, the presence of modified nucleotides has not been reported in its genome. We report here the detection of N6-methyladenine in the genome of D. radiodurans strain R1 using immunochemical techniques. This N6-methyladenine is not a part of GATC restriction-modification system. D. radiodurans cell extract also exhibited a DNA adenine methyltransferase activity which was reduced in the early post-irradiation recovery phase.
Prasad
Bhaskar Jyoti
BJ
Molecular Biology Research Laboratory, Department of Zoology, University of Pune, Pune 411007, India.
Sabnis
Ketaki
K
Deobagkar
Deepti D
DD
Deobagkar
Dileep N
DN
D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002474:Cell-Free System; D002845:Chromatography / Q000379:methods; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D019175:DNA Methylation; D004260:DNA Repair; D004269:DNA, Bacterial; D034301:Deinococcus / Q000235:genetics*; D016680:Genome, Bacterial; D007120:Immunochemistry; D010937:Plant Growth Regulators / Q000235:genetics; D016133:Polymerase Chain Reaction; D011827:Radiation; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000378:metabolism
D000225:Adenine; D004247:DNA; D004269:DNA, Bacterial; D010937:Plant Growth Regulators; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C000730567:6-methyladenine