title: [Effect of l-tetrahydropalmatine on expression of adhesion molecules induced by lipopolysaccharides in human umbilical vein endothelium cell].
abstract: OBJECTIVE
  To investigate the effect of l-tetrahydropalmatine (l-THP) on adhesion molecule expression induced by LPS in human umbilical vein endothelium cell (HUVEC).
  
  
  METHODS
  HUVEC were obtained from the human umbilical cord vein and treated by LPS or LPS plus different concentration of l-THP. Expression of ICAM-1 and E-selectin was assayed by flow cytometer.
  
  
  RESULTS
  l-THP(250 mg x L(-1)) markedly attenuated ICAM-1 and E-selectin expression induced by LPS(P <0.05). l-THP(50 mg x L(-1) inhibited E-selectin expression (P < 0.05) but had no effect on ICAM-1 (P > 0.05). l-THP (10 mg x L(-1)) had no effect on expression of both adhesion molecules. DXM (50 mg x L(-1)) completely inhibited both responses induced by LPS (P < 0.05).
  
  
  CONCLUSIONS
  l-THP depresses expression of ICAM-1 and E-selectin induced by LPS, which suggests that I-THP represent a promising candidate to be developed into therapies for the treatment of inflammation.
authors.affiliation: 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China. zzm681126@hotmail.com
authors.forename: Zhong-Miao
authors.initials: ZM
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Xiao-Xiang
authors.initials: XX
authors.lastname: Zheng
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D001600:Berberine Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D019040:E-Selectin / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001600:Berberine Alkaloids; D019040:E-Selectin; D008070:Lipopolysaccharides; D018799:Intercellular Adhesion Molecule-1; C014215:tetrahydropalmatine