title: The 2b protein as a minor structural component of PRRSV.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) ORF2 contains an internal ORF that codes for a small non-glycosylated protein known as 2b. Previous work had identified the presence of a 10kDa 2b protein in virus-infected cells and the induction of an anti-2b response in PRRSV-infected pigs, as well as a possible association of 2b with the virion (, Virology 287:183-191). In this study, we utilized two experimental approaches, including the use of a 2b peptide-specific monoclonal antibody, to demonstrate that the PRRSV 2b protein is an integral component of the PRRSV virion. This study suggests that 2b in PRRSV is similar to the E protein in EAV and forms a minor structural component of the virion.
authors.affiliation: Department of Veterinary Science, South Dakota State University, Box 2175, Brookings, SD 57007, USA.
authors.forename: Wai-Hong
authors.initials: WH
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.forename: Raymond R R
authors.initials: RR
authors.lastname: Rowland
authors.forename: Steven R
authors.initials: SR
authors.lastname: Lawson
authors.forename: Jane
authors.initials: J
authors.lastname: Christopher-Hennings
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Yoon
authors.forename: Eric A
authors.initials: EA
authors.lastname: Nelson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D047468:Immunoprecipitation; D019316:Porcine respiratory and reproductive syndrome virus / Q000737:chemistry* / Q000378:metabolism; D015678:Viral Structural Proteins / Q000276:immunology / Q000378:metabolism*; D014771:Virion / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015678:Viral Structural Proteins