title: Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass.
abstract: We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor alpha gene expressions were upregulated in SV-T2 and were thought to be candidates for call surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.
authors.affiliation: Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
authors.lastname: Tuoya
authors.forename: Koichi
authors.initials: K
authors.lastname: Hirayama
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Nagaoka
authors.forename: Dongwei
authors.initials: D
authors.lastname: Yu
authors.forename: Takayuki
authors.initials: T
authors.lastname: Fukuda
authors.forename: Hiroko
authors.initials: H
authors.lastname: Tada
authors.forename: Hidenori
authors.initials: H
authors.lastname: Yamada
authors.forename: Masaharu
authors.initials: M
authors.lastname: Seno
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins