title: Effect of parenteral vitamin E and selenium supplementation on immune status of dogs vaccinated with subunit and somatic antigens against Taenia hydatigena.
abstract: Thirty clinically healthy dogs were divided into five equal groups; Gs 1 and 2 were vaccinated with subunit and somatic antigens (Ags) respectively in combination with vitamin E and selenium (Vit E/Se) supplement, Gs 3 and 4 were vaccinated with subunit and somatic Ags respectively and group 5 was kept unvaccinated as control positive. Dogs in the vaccinated-Vit E/Se supplemented groups had a significantly greater (P < 0.05) serum Se and alpha-tocopherol than un-supplemented Gs. Best immune response against T. hydatigena was observed in the Vit E/Se supplemented groups, as evidenced by increase production of antibody titer and IgG concentration in comparison with either vaccinated un-supplemented or control groups. Moreover, the highest protection level against T. hydatigena infection was observed in groups 1 and 2 (83.3%), while was 66.7% and 50% in groups 3 and 4 respectively. Subunit Ag was more efficient than somatic Ag in improving the immune status of dogs. Vit E and Se proved to be immuno-potentiating to dogs vaccinated with subunit and somatic Ags and increased the possibility for the protection against T. hydatigena infection.
authors.affiliation: Department of Parasitology and Animal Diseases, National Research Center, Giza.
authors.forename: Omnia M
authors.initials: OM
authors.lastname: Kandil
authors.forename: Hala A A
authors.initials: HA
authors.lastname: Abou-Zeina
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000947:Antigens, Helminth / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology / Q000517:prevention & control; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007110:Immunity, Active / Q000187:drug effects; D010288:Parenteral Nutrition / Q000662:veterinary; D011897:Random Allocation; D012643:Selenium / Q000008:administration & dosage*; D013621:Taenia / Q000276:immunology*; D013622:Taeniasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D014611:Vaccination / Q000592:standards / Q000662:veterinary*; D014810:Vitamin E / Q000008:administration & dosage*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D014810:Vitamin E; D012643:Selenium