Inhibition of Colorado potato beetle larvae by a locust proteinase inhibitor peptide expressed in potato.
The cDNA for a 73-mer peptide containing two locust serine proteinase inhibitors was cloned, fused to the constitutive CaMV35S promoter and introduced into potato by Agrobacterium-mediated transformation. From 23 independent transgenic lines, three with high mRNA level and proteinase inhibitory activity were propagated in vitro and transferred to pots. The peptide from the leaves was identified by its N-terminal sequence and by K(i) values against chymotrypsin and trypsin. Colorado potato beetle larvae reared on transgenic plants grew slightly but significantly more slowly than those on control plants. This supports the notion that expression of multifunctional proteinase inhibitors of insect origin might be a good strategy to improve insect resistance in plants.
Kondrák
Mihály
M
Institute of Plant Biology, Agricultural Biotechnology Center, Gödöllo, Hungary.
Kutas
János
J
Szenthe
Borbála
B
Patthy
András
A
Bánfalvi
Zsófia
Z
Nádasy
Miklós
M
Gráf
László
L
Asbóth
Bence
B
D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D001517:Coleoptera / Q000254:growth & development*; D005247:Feeding Behavior / Q000502:physiology; D015870:Gene Expression; D006110:Grasshoppers / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics* / Q000502:physiology; D007814:Larva / Q000254:growth & development; D018515:Plant Leaves / Q000235:genetics / Q000469:parasitology; D030821:Plants, Genetically Modified; D011480:Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D011198:Solanum tuberosum / Q000235:genetics* / Q000469:parasitology; D013997:Time Factors
D019476:Insect Proteins; D011480:Protease Inhibitors