title: [Direct fluorescent and indirect immunofluorescent assay in buffy coat for candidemia diagnosis in pediatric patients: a comparative study].
abstract: The diagnosis of candidemia by blood culture has poor sensitivity; therefore, immunossupresed patients and those with risk factors may receive empirical antifungal therapy, wich is potentially toxic. Fluorescent tests have been developed to obtain an early and more sensitive diagnosis than blood culture. The aim of this study was to compare indirect immunofluorescence vs direct calcofluor white fluorescence in buffy coat for candidemia diagnosis. Sensitivity, specificity, predictive values, of positive and negative samples were 60%, 86%, 33%, 95% and 90%, 80%, 35%, 99%, for indirect immunofluorescence and calcofluor white, respectively.
authors.affiliation: Servicio de Infectología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Av. Cuauhtémoc 330, Col. Doctores Delegación Cuauhtémoc, México DF, CP 06780, México. drericflores@yahoo.com
authors.forename: Eric Moisés
authors.initials: EM
authors.lastname: Flores-Ruiz
authors.forename: María Guadalupe
authors.initials: MG
authors.lastname: Miranda-Novales
authors.forename: Fortino
authors.initials: F
authors.lastname: Solórzano-Santos
authors.forename: Gustavo
authors.initials: G
authors.lastname: Sánchez-Huerta
authors.forename: Humberto
authors.initials: H
authors.lastname: Díaz-Ponce
mesh_terms: D000293:Adolescent; D001557:Benzenesulfonates / Q000032:analysis; D002177:Candidiasis / Q000097:blood / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000032:analysis; D016469:Fungemia / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D016867:Immunocompromised Host; D007223:Infant; D007231:Infant, Newborn; D007962:Leukocytes / Q000382:microbiology*; D008297:Male; D008856:Microscopy, Fluorescence; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D001557:Benzenesulfonates; D005456:Fluorescent Dyes; C007061:C.I. Fluorescent Brightening Agent 28