Detection of two functional polymorphisms in the promoter region of the IL-18 gene by single-tube allele specific PCR and melting temperature analysis.
IL-18 is an important cytokine in both innate and acquired immunity. IL-18 promoter polymorphisms have been investigated in several autoimmune and infectious diseases. Here we present two assays for a medium to a high throughput genotyping of C-607A and G-137C IL-18 SNPs. Using these protocols we have been able to rapidly genotype a cohort of 131 healthy Italian individuals. The assays, based on a multiplex allele-specific PCR and the melting temperature analysis of the PCR product, are rapid, easily automated, inexpensive and well suited to case-control association studies.
Boniotto
Michele
M
Genetics Service, IRCCS Burlo Garofolo, Via dell'Istria 65/1, 34100 Trieste, Italy.
Segat
Ludovica
L
Milanese
Michele
M
Crovella
Sergio
S
D000483:Alleles; D002648:Child; D005838:Genotype; D006358:Hot Temperature; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
D020382:Interleukin-18