title: Expression and purification of N and E proteins from severe acute respiratory syndrome (SARS)-associated coronavirus: a comparative study.
abstract: Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, 300 Hsinchu, Taiwan.
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Lai
authors.forename: Yao-Chi
authors.initials: YC
authors.lastname: Chung
authors.forename: Yiu-Kay
authors.initials: YK
authors.lastname: Lai
authors.forename: Margaret Dah-Tsyr
authors.initials: MD
authors.lastname: Chang
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Hu
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000235:genetics; D002846:Chromatography, Affinity / Q000379:methods; D000086462:Coronavirus Nucleocapsid Proteins; D015870:Gene Expression; D018613:Microscopy, Confocal; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000378:metabolism*; D018411:Spodoptera; D013997:Time Factors; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D000086082:Viroporin Proteins
chemical_list: D000086462:Coronavirus Nucleocapsid Proteins; C501689:E protein, SARS coronavirus; D019590:Nucleocapsid Proteins; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D000086082:Viroporin Proteins