[Identification of genes that are specifically/preferentially expressed in developing cotton fibers by mRNA fluorescence differential display (FDD)].
Fluorescence differential display (FDD) technique was used to identify genes that are specifically or preferentially expressed in different developmental stages of cotton fiber cells. One hundred and nine differentially displayed cDNA fragments were isolated using 9, 21 and 27 DPA (days postanthesis) fibers as experimental materials. By a combination of two rounds of reverse Northern hybridization and Northern blot analyses, a number of such cDNA fragments were proved to represent fiber-specific/preferential genes. Sequencing determination and database searching indicated that most of these genes are novel. This work is an important step towards cloning the full-length cDNAs and characterizing the cellular functions of aforementioned genes in fiber development.
Sun
Jie
J
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Li
Yuan-Li
YL
Wang
Ruo-Hai
RH
Xia
Gui-Xian
GX
D015152:Blotting, Northern; D032561:Cotton Fiber; D005453:Fluorescence; D020869:Gene Expression Profiling / Q000379:methods*; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction