Determination of mono- to octachlorobiphenyls in fish oil using florisil adsorption followed by headspace solid-phase microextraction and gas chromatography with time-of-flight mass spectrometric detection.
A simple and reliable method for the determination of polychlorinated biphenyls (PCBs) from mono- to octachlorobiphenyls in fish oil for dietary supplement is described. The method combines Florisil clean up and headspace solid-phase microextraction on 65 microm polydimethylsiloxane-divinylbenzene (PDMS-DVB). Analyte detection was carried out using GC-time-of-flight mass spectrometry (GC-TOF-MS). Fifty three PCB congeners including the seven indicator PCBs (IUPAC Nos. 28, 52, 101, 118, 138, 153 and 180) were analyzed. Under optimal conditions, the method detection limit (MDL) of each congener in the range from 0.8 to 31 ng/g was found. A certified reference material (BCR-349) was analyzed and it showed good agreement with the certified data.
Agustin
Mary Rose R
MR
Advanced Analysis Center, Korea Institute of Science and Technology, P.O. Box 131 Cheongryangri, Seoul 130-650, South Korea.
Park
Hyun-Mee
HM
Hong
Soon-Min
SM
Ryu
Jae-Chun
JC
Lee
Kang-Bong
KB
D000327:Adsorption; D005395:Fish Oils / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D017633:Magnesium Silicates / Q000737:chemistry*; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results
D005395:Fish Oils; D017633:Magnesium Silicates; C005013:Florisil; D011078:Polychlorinated Biphenyls