title: Enterovirus RNA sequences in sera of schoolchildren in the general population and their association with type 1-diabetes-associated autoantibodies.
abstract: Type 1 diabetes (T1D) is an autoimmune disease linked with genetic factors as well as with environmental triggers, such as virus infections, but the aetiology is still unclear. The authors analysed serum from autoantibody-positive (n=50) and autoantibody-negative (n=50) schoolchildren as well as children newly diagnosed with T1D (n=47; time from diagnosis, median 5 days, interquartile range 1-12 days) for the presence and frequency of enterovirus (EV) and adenovirus sequences. The autoantibody-positive and -negative groups were part of the Karlsburg Type 1 Diabetes Risk Study of a Normal Schoolchild Population, which represents a general population without T1D first-degree relatives. There was no significant seasonality of sampling in any of the three groups investigated. EV RNA sequences were detected in 10 of 50 (20%) autoantibody-positive children and in 17 of 47 (36%) children newly diagnosed with T1D, but only in two of 50 (4%) of the age- and sex-matched controls (P<0.05, P<0.001). Characterization of the EV amplicons by direct sequencing revealed high homology with coxsackievirus B group. For adenovirus we found no data to support an association with T1D. The data support the hypothesis that different enteroviruses may be aetiologically important as a trigger and/or accelerating factor in the process of T1D development.
authors.affiliation: Friedrich Loeffler Institute of Medical Microbiology1 and Institute of Pathophysiology2, Ernst Moritz Arndt University of Greifswald, Germany 3Center of Diabetes and Metabolic Disorders, Karlsburg, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Moya-Suri
authors.affiliation: Friedrich Loeffler Institute of Medical Microbiology1 and Institute of Pathophysiology2, Ernst Moritz Arndt University of Greifswald, Germany 3Center of Diabetes and Metabolic Disorders, Karlsburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schlosser
authors.affiliation: Friedrich Loeffler Institute of Medical Microbiology1 and Institute of Pathophysiology2, Ernst Moritz Arndt University of Greifswald, Germany 3Center of Diabetes and Metabolic Disorders, Karlsburg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Zimmermann
authors.affiliation: Friedrich Loeffler Institute of Medical Microbiology1 and Institute of Pathophysiology2, Ernst Moritz Arndt University of Greifswald, Germany 3Center of Diabetes and Metabolic Disorders, Karlsburg, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Rjasanowski
authors.affiliation: Friedrich Loeffler Institute of Medical Microbiology1 and Institute of Pathophysiology2, Ernst Moritz Arndt University of Greifswald, Germany 3Center of Diabetes and Metabolic Disorders, Karlsburg, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Gürtler
authors.affiliation: Friedrich Loeffler Institute of Medical Microbiology1 and Institute of Pathophysiology2, Ernst Moritz Arndt University of Greifswald, Germany 3Center of Diabetes and Metabolic Disorders, Karlsburg, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Mentel
mesh_terms: D000293:Adolescent; D000914:Antibodies, Viral / Q000097:blood; D001323:Autoantibodies / Q000097:blood*; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000150:complications* / Q000821:virology; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000914:Antibodies, Viral; D001323:Autoantibodies; D012367:RNA, Viral