[Separation of correctly refolded and mis-refolded consensus interferon by hydrophobic interaction chromatography].
Hydrophobic interaction chromatography was used to separate correctly refolded and mis-refolded consensus interferon. The effects of ligand types, salt concentration, pH and flow rate were investigated. The best result could be obtained by using Butyl Sepharose 4 Fast Flow, 0.8 mol/L of ammonium sulfate, pH 8.3 and 90cm/h of linear flow rate. Reverse-phase HPLC analysis showed the purity of the pooled fraction was as high as 99.6%. The specific activity of purified consensus interferon was 2.3 x 10(9) IU/mg, The mass recovery of targeth protein was 36.7%.
Zhao
Rong-Zhi
RZ
Civil & Environmental Engineering School, University of Science and Technology of Beijing, Beijing 100083, China.
Liu
Yong-Dong
YD
Wang
Fang-Wei
FW
Li
Jing-Jing
JJ
Xia
Xin
X
Su
Zhi-Guo
ZG
D002853:Chromatography, Liquid / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D007370:Interferon Type I / Q000737:chemistry / Q000302:isolation & purification*; D016898:Interferon-alpha; D011487:Protein Conformation; D017510:Protein Folding; D011994:Recombinant Proteins
D007370:Interferon Type I; D016898:Interferon-alpha; D011994:Recombinant Proteins; C050739:interferon alfacon-1