title: Tumour necrosis factor alpha is expressed in refractory skin lesions from patients with subacute cutaneous lupus erythematosus.
abstract: OBJECTIVE
  To investigate whether tumour necrosis factor alpha (TNFalpha) is expressed in subacute cutaneous lupus erythematosus (SCLE) skin lesions.
  
  
  METHODS
  The in situ expression of TNFalpha in refractory lesional and non-lesional skin biopsy specimens from patients with SCLE was analysed using an immunohistochemical approach. At the time of biopsy these patients were receiving treatment with systemic medications such as antimalarial agents, immunosuppressive drugs, and thalidomide. Expression of TNFalpha was also evaluated in cutaneous lesions of patients with other inflammatory and neoplastic skin diseases as controls.
  
  
  RESULTS
  The data showed that refractory lesional skin tissue from patients with SCLE displays a strongly positive distribution of TNFalpha, particularly within the epidermis. No prominent staining was seen in non-lesional skin from the same group of patients or in cutaneous lesions from the control group.
  
  
  CONCLUSIONS
  These findings suggest that TNFalpha is localised and produced by epidermal cells within SCLE skin lesions and support its potential role in the pathogenesis of SCLE. The tissue localisation of TNFalpha may represent a potential therapeutic target providing a new perspective in the treatment of refractory skin lesions in patients with SCLE.
authors.affiliation: Department of Clinical and Experimental Medicine, University of Padova, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Zampieri
authors.forename: M
authors.initials: M
authors.lastname: Alaibac
authors.forename: L
authors.initials: L
authors.lastname: Iaccarino
authors.forename: R
authors.initials: R
authors.lastname: Rondinone
authors.forename: A
authors.initials: A
authors.lastname: Ghirardello
authors.forename: P
authors.initials: P
authors.lastname: Sarzi-Puttini
authors.forename: A
authors.initials: A
authors.lastname: Peserico
authors.forename: A
authors.initials: A
authors.lastname: Doria
mesh_terms: D000328:Adult; D018501:Antirheumatic Agents / Q000627:therapeutic use; D001323:Autoantibodies / Q000032:analysis; D004817:Epidermis / Q000276:immunology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008178:Lupus Erythematosus, Cutaneous / Q000188:drug therapy / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000473:pathology; D008875:Middle Aged; D012867:Skin / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D018501:Antirheumatic Agents; D001323:Autoantibodies; D014409:Tumor Necrosis Factor-alpha