title: [Differential regulation of phenazine-1-carboxylic acid and pyoluteorin production mediated by inactivated gacA in Pseudomonas sp. M18].
abstract: Pseudomonas sp. M18 is one of the plant-growth-promoting rhizobacteria, which can produce fungicides: phenazine-1-carboxylic acid (PCA) and pyoluteorin (Plt). The chromosomally gacA inactivated mutant named M18G was constructed and its PCA production was enhanced 31-fold and Plt production was almost blocked completely in KMB medium. To assess the mutual influence of two antibiotics, the plt gene cluster mutant M18T and the phz gene cluster mutant M18GA were then constructed. Non-Plt-producing M18T could synthesize the same amount of PCA as wild type strain. Plt could not be detected in M18GA while PCA production was inhibited dramatically in it. Results indicate that promotion of PCA and inhibition of Plt production in M18G do result from the inactivation of gacA gene. It suggests that the production of antibiotics in strain M18 is differentially mediated by the gacA.
authors.affiliation: School of Life Science & Biotechnology, Shanghai JiaoTong University, Shanghai 200240, China. yihe-ge@yahoo.com
authors.forename: Yi-He
authors.initials: YH
authors.lastname: Ge
authors.forename: Xian-Qing
authors.initials: XQ
authors.lastname: Huang
authors.forename: Su-Lian
authors.initials: SL
authors.lastname: Wang
authors.forename: Xue-Hong
authors.initials: XH
authors.lastname: Zhang
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Xu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005659:Fungicides, Industrial / Q000378:metabolism*; D010619:Phenazines / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D011758:Pyrroles / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D005659:Fungicides, Industrial; C091100:GacA protein, Bacteria; D010619:Phenazines; D010636:Phenols; D011758:Pyrroles; C095503:pyoluteorin; C037165:1-phenazinecarboxylic acid