Potential use of a Yersinia ruckeri O1 auxotrophic aroA mutant as a live attenuated vaccine.
The aroA gene of Yersinia ruckeri, which encodes 5-enolpyruvylshikimate 3-phosphate synthase, was insertionally inactivated with a DNA fragment containing a kanamycin resistance determinant and reintroduced by allelic exchange into the chromosome of Y. ruckeri 21102 O1 by means of the suicide vector pIVET8. The Y. ruckeri aroA::Kan(r) mutant was highly attenuated when inoculated intraperitoneally into rainbow trout, with a 50% lethal dose of >5 x 10(7) CFU. The mutants were not recoverable from the internal organs 48 h post-inoculation or later. The vaccination of rainbow trout with the AroA mutant as a live vaccine conferred significant protection (relative percentage survival = 90%) against the pathogenic wild-type strain of Y. ruckeri.
Temprano
A
A
Departamento de Patología Animal (Sanidad Animal), Microbiología e Inmunología, University of León, Spain.
Riaño
J
J
Yugueros
J
J
González
P
P
de Castro
L
L
Villena
A
A
Luengo
J M
JM
Naharro
G
G
D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics* / Q000627:therapeutic use; D005393:Fish Diseases / Q000517:prevention & control*; D018014:Gene Transfer Techniques / Q000662:veterinary; D007928:Lethal Dose 50; D009154:Mutation / Q000235:genetics; D017686:Oncorhynchus mykiss; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary*; D015009:Yersinia Infections / Q000517:prevention & control / Q000662:veterinary*; D044124:Yersinia ruckeri / Q000235:genetics / Q000276:immunology*
D001428:Bacterial Vaccines