title: Specificity of a Bacteroides thetaiotaomicron marker for human feces.
abstract: A bacterial primer set, known to produce a 542-bp amplicon specific for Bacteroides thetaiotaomicron, generated this product in PCR with 1 ng of extracted DNA from 92% of 25 human fecal samples, 100% of 20 sewage samples, and 16% of 31 dog fecal samples. The marker was not detected in 1 ng of fecal DNA from 61 cows, 35 horses, 44 pigs, 24 chickens, 29 turkeys, and 17 geese.
authors.affiliation: Dept. of Veterinary Pathobiology, 201 Connaway Hall, University of Missouri, Columbia, MO 65211, USA. carsonc@missouri.edu
authors.forename: C Andrew
authors.initials: CA
authors.lastname: Carson
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Christiansen
authors.forename: Helen
authors.initials: H
authors.lastname: Yampara-Iquise
authors.forename: Verel W
authors.initials: VW
authors.lastname: Benson
authors.forename: Claire
authors.initials: C
authors.lastname: Baffaut
authors.forename: Jerri V
authors.initials: JV
authors.lastname: Davis
authors.forename: Robert R
authors.initials: RR
authors.lastname: Broz
authors.forename: William B
authors.initials: WB
authors.lastname: Kurtz
authors.forename: Wendi M
authors.initials: WM
authors.lastname: Rogers
authors.forename: William H
authors.initials: WH
authors.lastname: Fales
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D001439:Bacteroides / Q000235:genetics*; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D004285:Dogs; D005243:Feces / Q000382:microbiology*; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D012680:Sensitivity and Specificity; D012722:Sewage / Q000382:microbiology; D014876:Water Pollution / Q000032:analysis*
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers; D012722:Sewage