Identification of differentially expressed genes HSPC016 in dermal papilla cells with aggregative behavior.
The dermal papilla plays pivotal roles in hair follicle cycle and dermal papilla cells (DPCs) with aggregative behavior have more obviously inductive ability. In the present study, the suppression subtractive hybridization method was employed to identify the differentially expressed genes in dermal papillae cells with aggregative behavior. Following mRNA isolation of DPC with and without aggregative behavior, cDNA of both populations were prepared and subtracted by suppression PCR. Sequencing of enriched cDNAs identified five genes differentially expressed including capping protein, paladin, and vascular endothelial growth factor. Interestingly, HSPC016, first cloned from CD34+ hematopoietic stem/progenitor cells (HSPC), was identified by SSH, cDNA dot blot and Northern blot, which showed that this gene was differentially expressed in DPC with aggregative behavior. The full-length cDNA of HSPC016 was shown to be 366 bp, and the possible function of HSPC016 related to transcriptional regulation.
Zhiqiang
Song
S
Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, People's Republic of China. songzq@mail.tmmu.com.cn
Jiwen
Wang
W
Fei
Hao
H
Weibin
Yang
Y
Feng
Zou
Z
Xichuan
Yang
Y
Chunlan
Li
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D002449:Cell Aggregation; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D018859:Hair Follicle / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D011506:Proteins; C502622:TMA7 protein, human