title: [Detecting the DNA damage of N-ras gene induced by potassium dichromate].
abstract: OBJECTIVE
  Applying RDPCR to detect the DNA damage of N-ras gene induced by Potassium dichromate
  
  
  METHODS
  Preparing single-stranded probes of exon 1 of N-ras gene in human. The genomic DNA was treated with Potassium dichromate, then amplified by RDPCR and detected by Southern hybridization with the probe.
  
  
  RESULTS
  The clear hybridized bands can be seen in the position which is induced by Potassium dichromate on the dose of 100 micromol/L, but can't be detected on the dose over 1000 mol/L.
  
  
  CONCLUSIONS
  It indicates that Potassium dichromate can cause the DNA damage of N-ras gene, which should be the key point of its carcinogenesis mechanisms.
authors.affiliation: Department of Environmental Health, School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Chao-Long
authors.initials: CL
authors.lastname: Rao
authors.forename: Zheng-Chang
authors.initials: ZC
authors.lastname: Heng
mesh_terms: D004249:DNA Damage; D004785:Environmental Pollutants / Q000633:toxicity*; D011905:Genes, ras; D006801:Humans; D011192:Potassium Dichromate / Q000633:toxicity*
chemical_list: D004785:Environmental Pollutants; D011192:Potassium Dichromate