Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA).
Cell/tissue-specific gene expression are tightly regulated by various combinations of multiple transcription factors (TFs). Here, we present an oligonucleotide array-based transcription factor assay (OATFA), which allows the simultaneous assay of multiple active TFs. In this proof-of-principle work, both purified TFs and cell extracts were analyzed using OATFA and further antibody-based validation confirmed the chip data. This method could simplify the assay of multiple TFs and may facilitate high-throughput profiling of large numbers of TFs.
Shao
Wei
W
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Wei
Hua-Jiang
HJ
Qiao
Ji-Ying
JY
Zhao
Yong-Chao
YC
Sun
Yi-Min
YM
Zhou
Yu-Xiang
YX
Cheng
Jing
J
D001681:Biological Assay / Q000379:methods; D002467:Cell Nucleus / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D014157:Transcription Factors / Q000032:analysis*
D014157:Transcription Factors