[Effect of wortmannin on endothelial cell proliferation and migration induced by high glucose Müller cell conditioned medium].
OBJECTIVE
To investigate the effect of wortmannin on endothelial cells proliferation and migration induced by high glucose Müller cell conditioned medium (HGMCM).


METHODS
Immunofluorescence, flow cytometry and Boyden chamber migration models were used to analyze the effect of wortmannin on endothelial cells.


RESULTS
50 nmol/L wortmannin could significantly inhibit the proliferation and migration of endothelial cells induced by HGMCM. The percentage of endothelial cells in S phase was obviously decreased [from (37.82 +/- 0.57)% to (21.91 +/- 0.23)%, P < 0.01], while there was an increase in the percentage of cells in G(0)/G(1) phase [from (54.57 +/- 1.19)% to (65.59 +/- 0.41)%, < 0.01] and G(2)/M phase (< 0.05).


CONCLUSIONS
Wortmannin can inhibit proliferation and migration of endothelial cells induced by HGMCM, suggesting that wortmannin carries an anti-angiogenetic ability in diseased retina.
Wang
Yue-hui
YH
The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China.
Wang
Xi-yuan
XY
Wang
Xin-rui
XR
Ni
Jin-song
JS
Xin
Ying
Y
Shi
Bo
B
Wu
Jia-xiang
JX
D000730:Androstadienes / Q000494:pharmacology*; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D005947:Glucose; D006801:Humans; D008297:Male; D009389:Neovascularization, Pathologic; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011817:Rabbits; D012160:Retina / Q000166:cytology*; D000077191:Wortmannin
D000730:Androstadienes; D017077:Culture Media, Conditioned; D000081082:Phosphoinositide-3 Kinase Inhibitors; D005947:Glucose; D000077191:Wortmannin