title: Characterization and primer development for amplification of chloroplast microsatellite regions of Fraxinus excelsior.
abstract: This study reports the characterization of chloroplast DNA (cpDNA) variation of Fraxinus excelsior at five loci and the successful development of primer pairs for the amplification of three of these containing mononucleotide microsatellites. We detected high levels of haplotype variation among provenances of Fraxinus around Europe and within Ireland.
authors.affiliation: Department of Botany, University of Dublin, Trinity College, Ireland. mharbourne@hotmail.com
authors.forename: M E
authors.initials: ME
authors.lastname: Harbourne
authors.forename: G C
authors.initials: GC
authors.lastname: Douglas
authors.forename: S
authors.initials: S
authors.lastname: Waldren
authors.forename: T R
authors.initials: TR
authors.lastname: Hodkinson
mesh_terms: D001483:Base Sequence; D002736:Chloroplasts / Q000235:genetics*; D017931:DNA Primers; D031661:Fraxinus / Q000235:genetics*; D018745:Genome, Plant; D018895:Microsatellite Repeats / Q000235:genetics*
chemical_list: D017931:DNA Primers