MicroRNA gene expression deregulation in human breast cancer.
MicroRNAs (miRNAs) are a class of small noncoding RNAs that control gene expression by targeting mRNAs and triggering either translation repression or RNA degradation. Their aberrant expression may be involved in human diseases, including cancer. Indeed, miRNA aberrant expression has been previously found in human chronic lymphocytic leukemias, where miRNA signatures were associated with specific clinicobiological features. Here, we show that, compared with normal breast tissue, miRNAs are also aberrantly expressed in human breast cancer. The overall miRNA expression could clearly separate normal versus cancer tissues, with the most significantly deregulated miRNAs being mir-125b, mir-145, mir-21, and mir-155. Results were confirmed by microarray and Northern blot analyses. We could identify miRNAs whose expression was correlated with specific breast cancer biopathologic features, such as estrogen and progesterone receptor expression, tumor stage, vascular invasion, or proliferation index.
Iorio
Marilena V
MV
Comprehensive Cancer Center, Ohio State University, Columbus, Ohio 43210, USA.
Ferracin
Manuela
M
Liu
Chang-Gong
CG
Veronese
Angelo
A
Spizzo
Riccardo
R
Sabbioni
Silvia
S
Magri
Eros
E
Pedriali
Massimo
M
Fabbri
Muller
M
Campiglio
Manuela
M
Ménard
Sylvie
S
Palazzo
Juan P
JP
Rosenberg
Anne
A
Musiani
Piero
P
Volinia
Stefano
S
Nenci
Italo
I
Calin
George A
GA
Querzoli
Patrizia
P
Negrini
Massimo
M
Croce
Carlo M
CM
D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*
D035683:MicroRNAs