title: Murine model of buckwheat allergy by intragastric sensitization with fresh buckwheat flour extract.
abstract: Food allergies affect about 4% of the Korean population, and buckwheat allergy is one of the most severe food allergies in Korea. The purpose of the present study was to develop a murine model of IgE-mediated buckwheat hypersensitivity induced by intragastric sensitization. Young female C3H/HeJ mice were sensitized and challenged intragastricly with fresh buckwheat flour (1, 5, 25 mg/dose of proteins) mixed in cholera toxin, followed by intragastric challenge. Anaphylactic reactions, antigen-specific antibodies, splenocytes proliferation assays and cytokine productions were evaluated. Oral buckwheat challenges of sensitized mice provoked anaphylactic reactions such as severe scratch, perioral/periorbital swellings, or decreased activity. Reactions were associated with elevated levels of buckwheatspecific IgE antibodies. Splenocytes from buckwheat allergic mice exhibited significantly greater proliferative responses to buckwheat than non-allergic mice. Buckwheat-stimulated IL-4, IL-5, and INF-gamma productions were associated with elevated levels of buckwheat-specific IgE in sensitized mice. In this model, 1 mg and 5 mg dose of sensitization produced almost the same degree of Th2-directed immune response, however, a 25 mg dose showed blunted antibody responses. In conclusion, we developed IgE-mediated buckwheat allergy by intragastric sensitization and challenge, and this model could provide a good tool for future studies.
authors.affiliation: Department of Pediatrics, Ajou University School of Medicine, Suwon, Korea. jsjs87@ajou.ac.kr
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Sejo
authors.initials: S
authors.lastname: Oh
authors.forename: Kisun
authors.initials: K
authors.lastname: Lee
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Jang
authors.forename: Myung-Hyun
authors.initials: MH
authors.lastname: Sohn
authors.forename: Kyoung-En
authors.initials: KE
authors.lastname: Lee
authors.forename: Kyu-Earn
authors.initials: KE
authors.lastname: Kim
mesh_terms: D000707:Anaphylaxis / Q000097:blood / Q000276:immunology; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D019612:Fagopyrum / Q000276:immunology*; D005260:Female; D005433:Flour; D005512:Food Hypersensitivity / Q000097:blood / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D051379:Mice; D008809:Mice, Inbred C3H; D010936:Plant Extracts / Q000008:administration & dosage / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013270:Stomach / Q000187:drug effects / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: D007074:Immunoglobulin G; D015848:Interleukin-5; D010936:Plant Extracts; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma