title: [In situ hybridization analysis of mRNA expression of 5-Hydroxytryptamine4 receptor in intestinal mucosa mast cells].
abstract: OBJECTIVE
  To verify 5-HT(4) receptor mRNA expression in intestinal mucosa mast cells (IMMC).
  
  
  METHODS
  IMMC was isolated from the whole intestines of normal rats by collagenase digestion and purified by percoll. In situ hybridization was performed to detect the expression of 5-HT(4) receptor mRNA in IMMC.
  
  
  RESULTS
  Evidently positive staining was shown in the cytoplasm of IMMC.
  
  
  CONCLUSIONS
  We have verified the expression of 5-HT(4) receptor mRNA in IMMC for the first time and provided evidence for further research.
authors.affiliation: Department of Gastroenterology, Peking University First Hospital, Beijing 100034, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chi
authors.forename: Xin-guang
authors.initials: XG
authors.lastname: Liu
mesh_terms: D000818:Animals; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D011985:Receptors, Serotonin