title: Antibodies to cerebellar nerve fibres in two patients with paraneoplastic cerebellar ataxia.
abstract: The aim of this study was to characterize two new atypical anti-neuronal antibodies using an immunohistochemical method on rat cerebellum and Western blot techniques with primate cerebellar tissue and with recombinant neuronal proteins. Atypical sera from two patients with paraneoplastic neurological syndromes associated with different tumours were detected. Case number 1 presented cerebellar degeneration and Merkel cell carcinoma and case number 2 paraneoplastic brainstem encephalitis and malignant fibrous histiocytoma. By immunohistochemistry, the two new atypical antibodies showed a similar fibrillar positivity in the molecular and granular layers and around the Purkinje cells. The dot blot with recombinant neuronal proteins (HuD, NOVA-1, CDR62/Yo, Amphiphysin) was negative, whereas the Western blot with neuronal antigens of primate cerebellum identified two different proteins with molecular weights (64 kD in case number 1, and 70 kD in case number 2). In conclusion, the two new antibody reactivities against nerve fibres should be integrated into the diagnostic paraneoplastic neurological syndromes guidelines.
authors.affiliation: Department of Animal Biology, University of Pavia, Pavia, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Balegno
authors.forename: Mauro
authors.initials: M
authors.lastname: Ceroni
authors.forename: Manuel
authors.initials: M
authors.lastname: Corato
authors.forename: Diego
authors.initials: D
authors.lastname: Franciotta
authors.forename: Bruno
authors.initials: B
authors.lastname: Giometto
authors.forename: Kalliope
authors.initials: K
authors.lastname: Marinu-Aktipu
authors.forename: Rosanna
authors.initials: R
authors.lastname: Nano
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood*; D001369:Axons / Q000276:immunology / Q000473:pathology; D015266:Carcinoma, Merkel Cell / Q000097:blood / Q000276:immunology; D002524:Cerebellar Ataxia / Q000097:blood / Q000276:immunology*; D002531:Cerebellum / Q000276:immunology* / Q000473:pathology; D003712:Dendrites / Q000276:immunology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000276:immunology*; D020362:Paraneoplastic Cerebellar Degeneration / Q000097:blood / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012878:Skin Neoplasms / Q000097:blood / Q000276:immunology
chemical_list: D000906:Antibodies