title: Platelet and granulocyte alloimmunisation in multitransfused Tunisian patients.
abstract: We determined the frequency of post-transfusion alloimmunisation against platelet and granulocyte antigens in 51 Tunisian polytransfused patients with haematological diseases. Serum samples were analysed by a standard and an antiglobulin-augmented lymphocytotoxicity technique, a granulocyte agglutination test, a granulocyte immunofluorescence test, a platelet immunofluorescence test and the monoclonal antibody-specific immobilisation of platelet antigens assay. No granulocyte-specific antibodies were detected. HLA antibodies were found in 58.8% of patients. Platelet-specific antibodies were detected in four patients and were directed against human platelet antigen (HPA)-5b, HPA-1b and HPA-3a. The three patients with Glanzmann's thrombasthenia developed anti-GPIIb/IIIa antibodies. This study provides immunogenetic information that could improve the management of transfusion therapy in Tunisia.
authors.affiliation: Laboratoire d'Hématologie et Banque du Sang, CHU Sahloul, Sousse, Tunisie.
authors.forename: Hadef
authors.initials: H
authors.lastname: Skouri
authors.forename: Ali
authors.initials: A
authors.lastname: Bettaieb
authors.forename: Patricia
authors.initials: P
authors.lastname: Fromont
authors.forename: Halima
authors.initials: H
authors.lastname: Elomri
authors.forename: Souad
authors.initials: S
authors.lastname: Ennabli
authors.forename: Philippe
authors.initials: P
authors.lastname: Bierling
mesh_terms: D001323:Autoantibodies / Q000097:blood; D001792:Blood Platelets / Q000276:immunology*; D006098:Granulocytes / Q000276:immunology* / Q000637:transplantation; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D017713:Platelet Transfusion; D014416:Tunisia
chemical_list: D001323:Autoantibodies; D006680:HLA Antigens