Effect of resveratrol on angiogenesis and platelet/fibrin-accelerated tumor growth in the chick chorioallantoic membrane model.
We carried out this investigation to examine the effects on angiogenesis-mediated processes and to define anti-angiogenesis mechanisms for flavonoids. We examined the effects and mechanisms of the flavonoid resveratrol on angiogenesis and tumor growth using the chick chorioallantoic membrane (CAM) model of angiogenesis, the CAM tumor growth model, and the effect on p53 in fibroblast growth factor-2 (FGF2) stimulated human endothelial cells using immunoassay. Resveratrol demonstrated potent inhibition (effective dose50=0.7+/-0.1 microM) of FGF2-induced angiogenesis and tumor growth. Furthermore, resveratrol significantly (P<0.01) inhibited platelet/fibrin clot-promoted human colon and fibrosarcoma tumor growth in the CAM tumor model. Resveratrol in a concentration-dependent (1-3 microM) manner significantly promoted apoptosis in FGF2-stimulated endothelial cells by increasing p53 protein production. These data indicated potent anti-angiogenesis efficacy, inhibition of tumor growth, and clot-mediated enhanced tumor growth. These data suggest potential anticancer benefits as a chemopreventive and chemotherapeutic for the flavonoid resveratrol.
Mousa
Sarah S
SS
Pharmaceutical Research Institute at Albany, NY 12208, USA.
Mousa
Shaymaa S
SS
Mousa
Shaker A
SA
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D003110:Colonic Neoplasms / Q000473:pathology / Q000517:prevention & control; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D016222:Fibroblast Growth Factor 2; D005354:Fibrosarcoma / Q000473:pathology / Q000517:prevention & control; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
D020533:Angiogenesis Inhibitors; D005419:Flavonoids; D010975:Platelet Aggregation Inhibitors; D013267:Stilbenes; D016159:Tumor Suppressor Protein p53; D016222:Fibroblast Growth Factor 2; D000077185:Resveratrol