Identification of genes involved in cytochrome c biogenesis in Shewanella oneidensis, using a modified mariner transposon.
A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon insertions in the cytochrome c maturation genes ccmC and ccmF1, respectively, were analyzed. Both mutants were deficient in anaerobic respiration and cytochrome c production.
Bouhenni
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R
Department of Biological Sciences, University of Wisconsin-Milwaukee, 3209 N. Maryland Ave., Milwaukee, WI 53211, USA.
Gehrke
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A
Saffarini
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D
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D045304:Cytochromes c / Q000096:biosynthesis*; D004251:DNA Transposable Elements / Q000235:genetics*; D015247:Deoxyribonuclease HindIII / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D017422:Sequence Analysis, DNA; D020592:Shewanella / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism
D001426:Bacterial Proteins; C116246:CcmC protein, bacteria; D004251:DNA Transposable Elements; D008565:Membrane Proteins; D045304:Cytochromes c; D015247:Deoxyribonuclease HindIII