[Experimental research on effect of human mesenchymal stem cells induced by shenqi fuzheng injection in cerebral infarction].
OBJECTIVE
To investigate the effect of shenqi fuzheng injection (SFI) in inducing differentiation of human mesenchymal stem cells (hMSCs) in brain stem and its effect on nervous function in model rats of cerebral infarction.


METHODS
Middle cerebral artery occlusion model rats were made, and hMSCs was injected into their brain after being amplified in vitro and incubated with SFI for 0.5 h, then the survival, migration and differentiation of hMSCs in brain stem as well as the change of nervous function in model rats were observed.


RESULTS
The post-transplantation reject reaction to hMSCs was low, it could survive as long as 6 weeks or more. No difference in area of infarction was shown before and after transplantation. Immunohistochemical staining showed that hMSCs expressed human neuron specific enolase (NSE), neurofilament (NF) and glial fibrillary acid protein (GFAP). The limb-kinetic function and tactile perception were improved in the model rats.


CONCLUSIONS
SFI can induce hMSCs differentiate into neurons in vivo, and hMSCs may be the ideal germinal cells for treating cerebral infarction.
Yao
Xiao-li
XL
The First Hospital Affiliated to Sun Yat-sen University, Guangzhou. yeyaoxiaoli@sohu.com
Zhang
Cheng
C
Lu
Xi-lin
XL
Feng
Shanwei
S
Deng
Yubin
Y
Liu
Zuguo
Z
D000818:Animals; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division; D002478:Cells, Cultured; D002544:Cerebral Infarction / Q000209:etiology / Q000601:surgery*; D003470:Culture Media; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D016900:Neurofilament Proteins / Q000096:biosynthesis; D009474:Neurons / Q000166:cytology; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014183:Transplantation, Heterologous
D003470:Culture Media; D004365:Drugs, Chinese Herbal; D016900:Neurofilament Proteins; D010751:Phosphopyruvate Hydratase