A previously found thylakoid membrane protein of 14kDa (TMP14) is a novel subunit of plant photosystem I and is designated PSI-P.
We show that the thylakoid membrane phosphoprotein TMP14 is a novel subunit of plant photosystem I (PSI). Blue native/SDS-PAGE and sucrose gradient fractionation demonstrated the association of the protein exclusively with PSI. We designate the protein PSI-P. The presence of PSI-P subunit in Arabidopsis mutants lacking other PSI subunits was analyzed and suggested a location in the proximity of PSI-L, -H and -O subunits. The PSI-P protein was not differentially phosphorylated in state 1 and state 2.
Khrouchtchova
Anastassia
A
Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Copenhagen, Denmark.
Hansson
Maria
M
Paakkarinen
Virpi
V
Vainonen
Julia P
JP
Zhang
Suping
S
Jensen
Poul Erik
PE
Scheller
Henrik Vibe
HV
Vener
Alexander V
AV
Aro
Eva-Mari
EM
Haldrup
Anna
A
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins; D007525:Isoelectric Focusing; D008027:Light; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008970:Molecular Weight; D010766:Phosphorylation; D045331:Photosystem I Protein Complex / Q000737:chemistry* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism*; D020524:Thylakoids / Q000737:chemistry* / Q000378:metabolism
D029681:Arabidopsis Proteins; C504242:At2g46820 protein, Arabidopsis; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D021122:Protein Subunits