title: Unusual accumulation of demethylspheroidene in anaerobic-phototrophic growth of crtA-deleted mutants of Rhodovulum sulfidophilum.
abstract: Rhodovulum sulfidophilum produces carotenoids in the spheroidene pathway. Spheroidene monooxygenase, CrtA, catalyzes the conversion of spheroidene to spheroidenone. crtA-deleted mutants of R. sulfidophilum did not produce spheroidenone and demethylspheroidenone. In these mutants, the ratio of demethylspheroidene to spheroidene increased with exposure to light. One mutant exhibiting a spheroidene-predominant phenotype did not grow under anaerobic-light conditions and was devoid of bacteriochlorophyll a, even under semiaerobic-light conditions There was no difference in the growth of the mutants under aerobic-dark conditions. These data suggest that demethylspheroidene is important for photosynthesis in R. sulfidophilum.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, 565-0871, Japan.
authors.forename: Isamu
authors.initials: I
authors.lastname: Maeda
authors.forename: Hidenori
authors.initials: H
authors.lastname: Yamashiro
authors.forename: Daiki
authors.initials: D
authors.lastname: Yoshioka
authors.forename: Masanori
authors.initials: M
authors.lastname: Onodera
authors.forename: Shunsaku
authors.initials: S
authors.lastname: Ueda
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyasaka
authors.forename: Fusako
authors.initials: F
authors.lastname: Umeda
authors.forename: Masaya
authors.initials: M
authors.lastname: Kawase
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takaichi
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Yagi
mesh_terms: D000693:Anaerobiosis; D002338:Carotenoids / Q000378:metabolism*; D017353:Gene Deletion; D008027:Light; D006899:Mixed Function Oxygenases / Q000172:deficiency / Q000235:genetics; D010788:Photosynthesis; D041903:Rhodovulum / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: C005637:spheroidene; C104880:spheroidenone; D002338:Carotenoids; D006899:Mixed Function Oxygenases