title: Affordable, abbreviated roche monitor assay for quantification of human immunodeficiency virus type 1 RNA in plasma.
abstract: The cost for the Roche Monitor assay kit can be reduced 50% by using only the 1:1, 1:25, and 1:625 human immunodeficiency virus (HIV) and the 1:1 quantitation standard dilutions. This abbreviated test applied to 1,774 mostly African samples had results nearly identical to those obtained following the package insert instructions. To make this approach feasible, Roche would have to provide additional lysis buffer and master mix.
authors.affiliation: Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. fiscussa@med.unc.edu
authors.forename: Susan A
authors.initials: SA
authors.lastname: Fiscus
authors.forename: Shu
authors.initials: S
authors.lastname: Chen
authors.forename: Donald
authors.initials: D
authors.lastname: Hoover
authors.forename: Melissa G
authors.initials: MG
authors.lastname: Kerkau
authors.forename: Paul
authors.initials: P
authors.lastname: Alabanza
authors.forename: Som
authors.initials: S
authors.lastname: Siharath
authors.forename: John
authors.initials: J
authors.lastname: Schmitz
authors.forename: Newton
authors.initials: N
authors.lastname: Kumwenda
authors.forename: Taha E
authors.initials: TE
authors.lastname: Taha
mesh_terms: D005260:Female; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D011247:Pregnancy; D011446:Prospective Studies; D012367:RNA, Viral / Q000097:blood*; D011933:Reagent Kits, Diagnostic / Q000191:economics*; D019562:Viral Load
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic