Acute ileal inflammatory cytokine response induced by irradiation is modulated by subdiaphragmatic vagotomy.
Neural involvement plays a role in the genesis of the peripheral inflammatory process that contributes to the irradiation intestinal disorders. However, little is known about the role of vagus nerve in modulating inflammatory process in rat. Here, we have shown that the NF-kappaB activation was consistent with the acute overexpression of pro-inflammatory cytokines (IL- 1beta, TNF-alpha, IL-6) at 3, 6, and 12 h induced by whole-body irradiation (8 Gy). Subdiaphragmatic vagotomy reduced NF-kappaB activation and cytokine transcription in the early period post-irradiation. In contrast, vagotomy amplified overexpression of irradiation-induced anti-cytokines (IL-10, IL-1Ra) and of receptors involved in anti-inflammatory effects (IL- 1RII, TNFRII). These results show that the vagus nerve is a pro-inflammatory pathway in early irradiation-induced intestinal inflammation.
Linard
Christine
C
Institute for Radioprotection and Nuclear Safety, IRSN, B.P. no17, F-92262 Fontenay-aux- Roses Cedex, France. christine.linard@irsn.fr
Marquette
Christel
C
Clarençon
Didier
D
Galonnier
Monique
M
Mathieu
Jacques
J
Pennequin
Andre
A
Benderitter
Marc
M
Gourmelon
Patrick
P
D000818:Animals; D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects*; D007082:Ileum / Q000528:radiation effects*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors; D015167:Vagotomy, Truncal / Q000379:methods*; D014916:Whole-Body Irradiation / Q000379:methods*
D016207:Cytokines; D016328:NF-kappa B; D012333:RNA, Messenger; D018121:Receptors, Cytokine