Transformation of mouse fibroblasts alters the induction pattern of type I IFNs after virus infection.
Type I interferons (IFNs) have been shown to be involved in many immune defence and inflammatory responses. We here show that IFN-beta plays an absolute essential role in the efficient induction of all type I IFNs after infection of primary embryonic as well as primary adult fibroblasts with Sendai virus. In contrast, after immortalization of such fibroblasts with SV40 large T antigen, IFN-alpha4 can be induced independently of IFN-beta. However, efficient secretion of type I IFNs even in immortalized fibroblasts is only found when the complete signalling loop is induced by IFN-beta.
Samuelsson
Christofer V
CV
Molecular Immunology, GBF, German Research Centre for Biotechnology, Braunschweig, Germany. csa@gbf.de
Lienenklaus
Stefan
S
Müller
Peter P
PP
Zawatzky
Rainer
R
Hauser
Hansjörg
H
Weiss
Siegfried
S
D000818:Animals; D001681:Biological Assay; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism* / Q000821:virology; D007249:Inflammation; D007370:Interferon Type I / Q000096:biosynthesis*; D007372:Interferons / Q000378:metabolism; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029082:Sendai virus / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
D007370:Interferon Type I; D012333:RNA, Messenger; D012313:RNA; D007372:Interferons