Gene expression profiling of mouse host response to Listeria monocytogenes infection.
The purpose of this study was to evaluate gene expression profiles in the liver and blood for prediction of infection severity from Listeria monocytogenes (LM). Mice were injected with medium broth (control) or a nonlethal or lethal dose of LM and sacrificed 6 h later. Gene expression changes were determined using Affymetrix MGU74Av2 GeneChips and confirmed by real-time polymerase chain reaction analysis. We identified discernable genes whose gene expression profiles can be used in pattern recognition to predict and classify samples in differently treated groups, with >or=90% accuracy in liver samples and 80% accuracy in blood at prediction; however, different genes were predictive in each tissue. Our results suggest that gene expression profiling in response to LM in mice may be able to distinguish samples in groups with varying severity of infection and provide information in finding molecular mechanisms and early biomarkers for subsequent conventional clinical endpoints.
Ng
Hanna H
HH
Biosciences Division, SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493, USA. hanna.ng@sri.com
Frantz
Christopher E
CE
Rausch
Linda
L
Fairchild
David C
DC
Shimon
Julie
J
Riccio
Edward
E
Smith
Sherrod
S
Mirsalis
Jon C
JC
D000410:Alanine Transaminase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D016000:Cluster Analysis; D017931:DNA Primers; D005260:Female; D020869:Gene Expression Profiling; D005796:Genes / Q000235:genetics*; D008089:Listeria monocytogenes; D008088:Listeriosis / Q000097:blood* / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase