Multilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa.
A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, distributed across the X. fastidiosa genome, clearly differentiated and clustered X. fastidiosa strains collected from grape, almond, citrus, and oleander. They are well suited for differentiating strains and studying X. fastidiosa epidemiology and population genetics.
Lin
Hong
H
Crop Diseases, Pests & Genetics, U.S. Department of Agriculture-ARS, 9611 S. Riverbend Avenue, Parlier, CA 93648, USA. hlin@fresno.ars.usda.gov
Civerolo
Edwin L
EL
Hu
Rong
R
Barros
Samuel
S
Francis
Marta
M
Walker
M Andrew
MA
D001483:Base Sequence; D002957:Citrus / Q000382:microbiology; D017931:DNA Primers; D005819:Genetic Markers; D014644:Genetic Variation; D018598:Minisatellite Repeats / Q000235:genetics*; D030007:Nerium / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D027861:Prunus / Q000382:microbiology; D017422:Sequence Analysis, DNA; D027843:Vitis / Q000382:microbiology; D044167:Xylella / Q000145:classification* / Q000235:genetics
D017931:DNA Primers; D005819:Genetic Markers