title: Electroenzymatic degradation of azo dye using an immobilized peroxidase enzyme.
abstract: Azo dyes are largely resistant to biodegradation and persist in conventional wastewater treatment processes. Combining enzymatic catalysis and the electrochemical generation of hydrogen peroxide (H2O2), an electroenzymatic process was developed, which is a potential alternative to traditional processes. In this study, an electroenzymatic method that uses an immobilized horseradish peroxidase enzyme (HRP), was investigated to degrade orange II (azo dye) within a two-compartment packed-bed flow reactor. To evaluate the electroenzymatic degradation of orange II, electrolytic experiments were carried out with 0.42 U/mL HRP at -0.5 V. It was found that removal of orange II was partly due to its adsorption to the graphite felt. The overall application of the electroenzymatic led to a greater degradation rate than the use of electrolysis alone. Also the by-products formed were found to consist primarily of an aromatic amine, sulfanilic acid, and unknown compounds.
authors.affiliation: Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), 1 Oryong-dong, Buk-gu, Gwangju 500-712, South Korea.
authors.forename: Gha-Young
authors.initials: GY
authors.lastname: Kim
authors.forename: Ki-Beom
authors.initials: KB
authors.lastname: Lee
authors.forename: Seung-Hee
authors.initials: SH
authors.lastname: Cho
authors.forename: Joonmok
authors.initials: J
authors.lastname: Shim
authors.forename: Seung-Hyeon
authors.initials: SH
authors.lastname: Moon
mesh_terms: D001391:Azo Compounds / Q000737:chemistry*; D001557:Benzenesulfonates / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007220:Industrial Waste / Q000517:prevention & control*; D009994:Osmolar Concentration; D009195:Peroxidase / Q000737:chemistry*; D013783:Textile Industry; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*; D014877:Water Pollution, Chemical / Q000517:prevention & control*
chemical_list: D001391:Azo Compounds; D001557:Benzenesulfonates; D004396:Coloring Agents; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D006861:Hydrogen Peroxide; D009195:Peroxidase; C014722:2-naphthol orange