Study of fibrinogen adsorption on self-assembled monolayers on Au(111) by atomic force microscopy.
The adsorption of plasma protein fibrinogen on the self-assembled monolayers (SAMs) of n-hexadecyl mercaptan and citrate was investigated with atomic force microscopy (AFM). On the basis of the preparation of these two flat SAMs on Au(111), high-resolution AFM images of bovine fibrinogen were obtained with different protein concentrations. The results indicated that the surface chemical composition significantly affected the adsorption behavior of fibrinogen. Since fibrinogen plays a key role in the regulation of both haemostasis and thrombosis, high-resolution AFM imaging on SAMs is expected to be an effective approach to study the haemocompatibility of materials with different surface chemistry.
Lin
Yi
Y
Center of Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Wang
Jun
J
Wan
Li-Jun
LJ
Fang
Xiao-Hong
XH
D000327:Adsorption; D000818:Animals; D002417:Cattle; D005340:Fibrinogen / Q000493:pharmacokinetics*; D018625:Microscopy, Atomic Force; D012834:Silver / Q000737:chemistry*; D013499:Surface Properties
D012834:Silver; D005340:Fibrinogen