Simple and rapid method for cis- and trans-resveratrol and piceid isomers determination in wine by high-performance liquid chromatography using chromolith columns.
A simple and rapid HPLC method has been developed for simultaneous determination of the four resveratrol forms (aglycon and glycosidic) in a Grenache wine from Châteauneuf du Pape (Vaucluse). These analyses were achieved by using two commercial monolith HPLC columns and diode array detection. The method provided reliable separations at low pressure with a short analysis time. The limit of detection (LD) and limit of quantification (LQ) were calculated for each standard. The molecules were separated and quantified in a single run without any purification of the sample.
Vian
M Abert
MA
SARL Enigma, Département d'analyses biochimiques, Hameau de St. Veran, 84190 Beaumes de Venise, France.
Tomao
V
V
Gallet
S
S
Coulomb
P O
PO
Lacombe
J M
JM
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry; D000077185:Resveratrol; D013237:Stereoisomerism; D013267:Stilbenes / Q000032:analysis* / Q000737:chemistry; D014920:Wine / Q000032:analysis*
D005960:Glucosides; D013267:Stilbenes; D000077185:Resveratrol; C058229:polydatin