title: Analysis of protein interactions on protein arrays by a novel spectral surface plasmon resonance imaging.
abstract: We presented a novel surface plasmon resonance (SPR) imaging method for analysis of protein arrays based on a wavelength interrogation-based SPR biosensor. The spectral imaging was performed by the combination of position control and resonance wavelengths calculated from SPR reflectivity spectra. The imaging method was evaluated by analyzing interactions of glutathione S-transferase-fusion proteins with their antibodies. Antigen-antibody interactions were successfully analyzed on glutathione S-transferase-fusion protein arrays by using the spectral imaging method, and the results were confirmed by a parallel analysis using a previously used spectral SPR biosensor based on wavelength interrogation. Specific binding of anti-Rac1 and anti-RhoA to Rac1 and RhoA on the protein arrays was qualitatively and quantitatively analyzed by the spectral SPR imaging. Thus, it was suggested that the novel spectral SPR imaging was a useful tool for the high-throughput analysis of protein-protein interactions on protein arrays.
authors.affiliation: Department of Molecular and Cellular Biochemistry and Nano-Bio Sensor Research Center, Kangwon National University College of Medicine, Chunchon, South Korea.
authors.forename: Jong Seol
authors.initials: JS
authors.lastname: Yuk
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Jae-Wan
authors.initials: JW
authors.lastname: Jung
authors.forename: Se-Hui
authors.initials: SH
authors.lastname: Jung
authors.forename: Seung-Joon
authors.initials: SJ
authors.lastname: Lee
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Kim
authors.forename: Jeong-A
authors.initials: JA
authors.lastname: Han
authors.forename: Young-Myeong
authors.initials: YM
authors.lastname: Kim
authors.forename: Kwon-Soo
authors.initials: KS
authors.lastname: Ha
mesh_terms: D000936:Antigen-Antibody Complex / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D025941:Protein Interaction Mapping / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
chemical_list: D000936:Antigen-Antibody Complex