HPLC-DAD for analysis of different classes of drugs in plasma.
A solid-phase extraction technique and HPLC-DAD method for drug screening in plasma were developed. This analytical method extracts and tests a number of drugs of different classes. The method includes; an acidic and basic Solid Phase Extraction (SPE) of plasma with C18 cartridges, a gradient elution of a modified cyano column with acidic buffer/acetonitrile eluent and a photodiode array ultraviolet (UV) detection. The drug screening procedure applied uses retention index and UV spectral data for the identification of compounds, may be appropriate in particular laboratory settings.
Alabdalla
Mahmoud A
MA
Department of Chemical Analysis, Forensic Science Laboratory, Amman, Jordan. mah13669@hotmail.com
D000097:Acetonitriles; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D009294:Narcotics / Q000097:blood; D004364:Pharmaceutical Preparations / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D015813:Substance Abuse Detection / Q000379:methods
D000097:Acetonitriles; D009294:Narcotics; D004364:Pharmaceutical Preparations; C032159:acetonitrile