Cell cycle, DNA replication, repair, and recombination in the dimorphic human pathogenic fungus Paracoccidioides brasiliensis.
DNA replication, together with repair mechanisms and cell cycle control, are the most important cellular processes necessary to maintain correct transfer of genetic information to the progeny. These processes are well conserved throughout the Eukarya, and the genes that are involved provide essential information for understanding the life cycle of an organism. We used computational tools for data mining of genes involved in these processes in the pathogenic fungus Paracoccidiodes brasiliensis. Data derived from transcriptome analysis revealed that the cell cycle of this fungus, as well as DNA replication and repair, and the recombination machineries, are highly similar to those of the yeast Saccharomyces cerevisiae. Among orthologs detected in both species, there are genes related to cytoskeleton structure and assembly, chromosome segregation, and cell cycle control genes. We identified at least one representative gene from each step of the initiation of DNA replication. Major players in the process of DNA damage and repair were also identified.
Reis
Viviane Castelo Branco
VC
Laboratório de Biologia Molecular, Departamento de Biologia Celular, Universidade de Brasília, 70910-900 Brasília, DF, Brazil.
Torres
Fernando Araripe Gonçalves
FA
Poças-Fonseca
Marcio José
MJ
De-Souza
Marlene Teixeira
MT
Souza
Diorge Paulo de
DP
Almeida
João Ricardo Moreira
JR
Marinho-Silva
Camila
C
Parachin
Nádia Skorupa
NS
Dantas
Alessandra da Silva
Ada S
Mello-de-Sousa
Thiago Machado
TM
Moraes
Lídia Maria Pepe de
LM
D002453:Cell Cycle / Q000235:genetics* / Q000502:physiology; D004260:DNA Repair / Q000235:genetics* / Q000502:physiology; D004261:DNA Replication / Q000235:genetics* / Q000502:physiology; D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D006801:Humans; D009154:Mutation / Q000235:genetics; D010228:Paracoccidioides / Q000166:cytology / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic / Q000235:genetics
D004271:DNA, Fungal