Fractal intermediates in the self-assembly of silicatein filaments.
Silicateins are proteins with catalytic, structure-directing activity that are responsible for silica biosynthesis in certain sponges; they are the constituents of macroscopic protein filaments that are found occluded within the silica needles made by Tethya aurantia. Self-assembly of the silicatein monomers and oligomers is shown to form fibrous structures by a mechanism that is fundamentally different from any previously described filament-assembly process. This assembly proceeds through the formation of diffusion-limited, fractally patterned aggregates on the path to filament formation. The driving force for this self-assembly is suggested to be entropic, mediated by the interaction of hydrophobic patches on the surfaces of the silicatein subunits that are not found on highly homologous congeners that do not form filaments. Our results are consistent with a model in which silicatein monomers associate into oligomers that are stabilized by intermolecular disulfide bonds. These oligomeric units assemble into a fractal network that subsequently condenses and organizes into a filamentous structure. These results represent a potentially general mechanism for protein fiber self-assembly.
Murr
Meredith M
MM
Department of Molecular Cellular and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.
Morse
Daniel E
DE
D000818:Animals; D002403:Cathepsins / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D017709:Fractals; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D011161:Porifera / Q000737:chemistry; D011485:Protein Binding / Q000187:drug effects; D017510:Protein Folding; D020836:Protein Structure, Quaternary / Q000187:drug effects; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology
D021122:Protein Subunits; C112571:silicatein alpha; D012965:Sodium Chloride; D002403:Cathepsins