title: MtdC, a novel class of methylene tetrahydromethanopterin dehydrogenases.
abstract: Novel methylene tetrahydromethanopterin (H4MPT) dehydrogenase enzymes, named MtdC, were purified after expressing in Escherichia coli genes from, respectively, Gemmata sp. strain Wa1-1 and environmental DNA originating from unidentified microbial species. The MtdC enzymes were shown to possess high affinities for methylene-H4MPT and NADP but low affinities for methylene tetrahydrofolate or NAD. The substrate range and the kinetic properties revealed by MtdC enzymes distinguish them from the previously characterized bacterial methylene-H4MPT dehydrogenases, MtdA and MtdB. While revealing higher sequence similarity to MtdA enzymes, MtdC enzymes appear to fulfill a function homologous to the function of MtdB, as part of the H4MPT-linked pathway for formaldehyde oxidation/detoxification.
authors.affiliation: Laboratorie des Interactions Plantes-Microorganismes, 31326 Castanet-Tolosan, France.
authors.forename: Julia A
authors.initials: JA
authors.lastname: Vorholt
authors.forename: Marina G
authors.initials: MG
authors.lastname: Kalyuzhnaya
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Hagemeier
authors.forename: Mary E
authors.initials: ME
authors.lastname: Lidstrom
authors.forename: Ludmila
authors.initials: L
authors.lastname: Chistoserdova
mesh_terms: D001419:Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D000587:Oxidoreductases Acting on CH-NH Group Donors; C047126:methylenetetrahydromethanopterin dehydrogenase