Challenges facing the development and use of protein chips to analyze the phosphoproteome.
Recent advances in analytical methods, particularly in the area of protein microarrays, have brought the field of proteomics to the forefront of biological science. Protein arrays have shown to be useful for the multiplexed analysis of several hundreds of proteins in parallel. While much of the effort has focused on developing methods to identify expressed proteins, the identification of post-translational modifications is equally important for comprehensive proteome characterization. Protein phosphorylation constitutes a major type of post-translational modification that mobilizes a high number of genes, is involved in many crucial cell functions and largely contributes to the complexity of the proteome. One of the major challenges to analyze phosphoproteins using arrays is the availability of specific antibodies. Thus far, this has hampered the development of highly complex phosphoprotein arrays. This review discusses some of the recent progress made in the development of techniques and reagents to quantitatively determine sites of protein phosphorylation.
Beernink
Hans T H
HT
Immunology R&D, BioSource International, 542 Flynn Rd, Camarillo, CA 93012, USA. hansb@biosource.com
Nock
Steffen
S
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D010750:Phosphoproteins; D020543:Proteome