Flavonoid glycosides inhibit oral cancer cell proliferation--role of cellular uptake and hydrolysis to the aglycones.
Epidemiologic evidence supports the view that dietary flavonoids exert protective effects in oral diseases, including cancer. However, the dietary forms of flavonoids, the flavonoid glycosides, are thought to be inactive, thus they must first be hydrolysed to their active aglycones. This may occur in the saliva in the oral cavity. We have examined if the flavonoid glycosides directly could affect cell proliferation, using the human oral squamous carcinoma SCC-9 cells. The cellular uptake and hydrolysis of the glycosides were assessed also. The four flavonoid glycosides tested each behaved differently. Genistin, the 7-glucoside of genistein, showed clear and consistent inhibition of cell proliferation, which appeared to be the result of rapid cellular uptake of the glucoside and hydrolysis to genistein. Spiraeoside, the 4'-glucoside of quercetin, showed a similar inhibition of cell proliferation, which also appeared to be associated with its hydrolysis to quercetin. Diosmin, the 7-rutinoside of diosmetin, surprisingly, was more potent and effective than diosmetin. In contrast, quercitrin, the 3-rhamnoside of quercetin, showed no effect and only minimal cellular uptake and no hydrolysis. In summary, dietary flavonoid glycosides may exert cellular effects in the oral cavity, but this varies greatly with the nature of the glycoside.
Browning
Alyson M
AM
Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA.
Walle
U Kristina
UK
Walle
Thomas
T
D002294:Carcinoma, Squamous Cell; D045744:Cell Line, Tumor / Q000166:cytology / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004032:Diet; D004145:Diosmin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D007529:Isoflavones / Q000494:pharmacology*; D009062:Mouth Neoplasms; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology
D005419:Flavonoids; D006027:Glycosides; D007529:Isoflavones; C040641:genistin; C012526:quercitrin; D004145:Diosmin; D011794:Quercetin; C080613:spiraeoside; C039602:diosmetin