Isolation of the gene encoding the bovine neonatal Fc receptor.
The role of an IgG-Fc receptor (FcRn) that resembles a class I MHC Ag in transporting IgGs through epithelial cells was recently shown in selected species. Here we report our preliminary characterization of a clone encoding the alpha chain of the bovine FcRn from a BAC library. The recombinant BAC DNA was digested, analyzed by Southern blot hybridization and a bovine FcRn positive 9 kb long fragment was subcloned and partially sequenced. The exon/intron organization of the bovine FcRn alpha chain gene was deduced by comparison with its cDNA sequence. The sequence revealed a similar organization to the human and mouse FcRn alpha chain genes. The bovine FcRn alpha chain gene has acquired several repetitive sequences in its 5'-flanking region, including multiple SINE and LINE elements. Potential binding sites for transcription factors within the 5'-flanking sequence were identified using TESS and TFSEARCH programs. The 5'-flanking region of the bFcRn alpha chain gene was analyzed for its ability to directly express the luciferase reporter gene in bovine mammary gland epithelial cells. Transient transfection of a luciferase construct revealed that there was promoter activity in the region -1787 to +92 of the 5'-flanking sequence.
Doleschall
Márton
M
Department of Physiology and Biochemistry, Faculty of Veterinary Science, Szent Istvan University, P.O. Box 2, H-1400 Budapest, Hungary.
Zhao
Yaofeng
Y
Mayer
Balázs
B
Hammarström
Lennart
L
Kacskovics
Imre
I
D000818:Animals; D000831:Animals, Newborn; D001665:Binding Sites / Q000235:genetics; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005091:Exons; D005260:Female; D017930:Genes, Reporter; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D018928:Immunity, Mucosal; D007438:Introns; D020084:Long Interspersed Nucleotide Elements; D008321:Mammary Glands, Animal / Q000276:immunology; D051379:Mice; D011961:Receptors, Fc / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020086:Short Interspersed Nucleotide Elements; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
D015395:Histocompatibility Antigens Class I; D011961:Receptors, Fc; D011994:Recombinant Proteins; D014157:Transcription Factors; D004247:DNA; C100688:Fc receptor, neonatal