Genetic differentiation of charcoal rot pathogen, Macrophomina phaseolina, into specific groups using URP-PCR.
Forty isolates of Macrophomina phaseolina, a pathogen causing charcoal dry root rot of soybean, cotton, and chickpea, were genetically characterized with universal rice primers (URP; primers derived from DNA repeat sequences in the rice genome) using polymerase chain reaction (URP-PCR). Out of 12 URPs used in this study, 5 primers were effective in producing polymorphic fingerprint patterns from the DNA of M. phaseolina isolates. Three primers (URP-2F, URP-6R, and URP-30F) were quite informative and produced high levels of polymorphism among the isolates of M. phaseolina. Analysis of the entire fingerprint profiles using unweighted pair-group method with arithmetic averages (UPGMA) clearly differentiated M. phaseolina isolates obtained from soybean, cotton, and chickpea hosts into specific groups. In this study, we found for the first time transferability and use of PCR primers derived from plant genomes to generate host-specific fingerprint profiles of M. phaseolina, a broad host range plant pathogenic fungus. These results demonstrate that URPs are sensitive and technically simple to use for assaying genetic variability in M. phaseolina populations.
Jana
T K
TK
National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, Pusa, New Delhi, India.
Singh
N K
NK
Koundal
K R
KR
Sharma
T R
TR
D001203:Ascomycota / Q000145:classification* / Q000235:genetics*; D029863:Cicer / Q000382:microbiology; D017931:DNA Primers; D014644:Genetic Variation; D018745:Genome, Plant; D003368:Gossypium / Q000382:microbiology; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D012275:Oryza / Q000235:genetics*; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D013025:Glycine max / Q000382:microbiology; D013045:Species Specificity
D017931:DNA Primers