Functional analysis of fengycin synthetase FenD.
Fengycin is a cyclic lipopeptidic antibiotic produced nonribosomally by Bacillus subtilis. A fengycin synthetase mutant of B. subtilis F29-3 was generated with Tn917lux, which contains a transposon inserted in a 7716-bp gene, fenD. The mutation can be genetically complemented by transforming a plasmid carrying a wild-type fenD, confirming the participation of the gene in fengycin synthesis. Sequencing and biochemical analysis reveal that this gene encodes an enzyme that includes two amino acid-activating modules, FenD1 and FenD2, which activate l-Tyr and l-Thr, the third and the fourth amino acids in fengycin, respectively.
Lin
Tsuey-Pin
TP
Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan 701, Taiwan.
Chen
Chyi-Liang
CL
Fu
Hui-Chuan
HC
Wu
Cheng-Yeu
CY
Lin
Guang-Huey
GH
Huang
Shih-Hao
SH
Chang
Li-Kwan
LK
Liu
Shih-Tung
ST
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics*; D017124:Conserved Sequence; D004926:Escherichia coli / Q000235:genetics; D055666:Lipopeptides; D008074:Lipoproteins / Q000096:biosynthesis; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D046908:Peptide Biosynthesis, Nucleic Acid-Independent; D010453:Peptide Synthases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids
D055666:Lipopeptides; D008074:Lipoproteins; C049972:fengycin; D010453:Peptide Synthases; C110972:fengycin synthetase