[Construction of mutant population of differential race of Xa23 resistant to rice bacterial blight and avirulence activity identification of mutants].
The mutant population of Xanthomonas oryzae pv oryzae strain differential to rice bacterial blight resistance gene Xa23 has been constructed mediated by transposon in vivo . The results of PCR amplification with specific primers and analysis of flanking sequence of mutants indicated that the foreign DNA has been integrated into X. oryzae pv oryzae genome. Four mutants with changed avirulent activity to Xa23 gene have been identified by artificial inoculation. It is possible to clone genes that are required for AvrXa23 avirulence activity using this new strategy.
Zhou
Yong-Li
YL
Key Laboratory of Crop Genetic and Breeding of Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Pan
Ya-Jiao
YJ
Zhai
Wen-Xue
WX
Xu
Jian-Long
JL
Zhang
Qi
Q
Li
Zhi-Kang
ZK
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004251:DNA Transposable Elements; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000382:microbiology; D014774:Virulence; D014974:Xanthomonas / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology
D001426:Bacterial Proteins; D004251:DNA Transposable Elements