Changes in mussel Mytilus galloprovincialis protein profile as a reaction of water pollution.
In the present report the changes in mussels Mytilus galloprovincialis protein profile, as a reaction of water pollution, is presented. The antioxidant capacity was determined by the efficacy to scavenge the derived radical in mussel samples using the Trolox Equivalent Antioxidant Capacity (TEAC) assay. The highest percentage of inhibition was estimated in protein mussels from the dirty area (DM) in comparison with the clean samples (CM). The amounts of both zinc and copper determined by atomic spectroscopy were significantly higher in the protein mussels from the dirty than from the clean areas (P<0.05). Fourier Transform Infrared (FT-IR) spectroscopy and fluorescence revealed specific qualitative changes in secondary and tertiary structures of mussel proteins from the dirty area by the shift in the amides I and II positions and fluorescence intensity. Estimated temperature and enthalpy of denaturation in the protein mussels well correlated with fluorescence and spectroscopic measurements and showed the changes influenced by water pollution.
Gorinstein
Shela
S
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University-Hadassah Medical School, Jerusalem, Israel. gorin@cc.huji.ac.il
Arancibia-Avila
Patricia
P
Moncheva
Snejana
S
Toledo
Fernando
F
Trakhtenberg
Simon
S
Gorinstein
Arkadi
A
Goshev
Ivan
I
Namiesnik
Jacek
J
D000818:Animals; D002151:Calorimetry; D016166:Free Radical Scavengers / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology; D049878:Mytilus / Q000187:drug effects* / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
D016166:Free Radical Scavengers; D008670:Metals; D011506:Proteins; D014874:Water Pollutants, Chemical