title: Biochemical characterization of rat intestine development using high-resolution magic-angle-spinning 1H NMR spectroscopy and multivariate data analysis.
abstract: We report details of metabolic profiles for small intestinal samples obtained using high-resolution magic-angle-spinning (HRMAS) (1)H NMR spectroscopy. Intact samples of jejunum and ileum from male Long Evans rats were analyzed on a 600 MHz spectrometer using standard one and two-dimensional (1)H NMR spectroscopic pulse sequences. The metabolic profiles of ileum and jejunum predominantly comprised a number of amino acids, lipids, glycerophosphocholine (GPC), choline, creatine, and ethanol, a number of carboxylic acids including acetate and lactate, and nucleoside bases including cytosine, isocytosine, and uracil. Principal component analysis (PCA) was applied to these NMR data to characterize the biochemical differences between jejunum and ileum tissues. Compared with ileum, jejunum contained higher levels of lipids, GPC, choline, lactate and creatinine, but lower levels of amino acids and acetate. In addition, the age dependence of the biochemical composition of intestinal tissues from young rats (15, 36 days and 3-4 months old) was studied. In general, levels of lipids, lactate, taurine and creatinine were positively correlated with age while amino acids and GPC decreased in the older age group. This study will provide a metabolic reference for further studies assessing the metabolic consequences of nutrition, stress and gut microbiota on intestinal composition.
authors.affiliation: Biological Chemistry, Biomedical Sciences Division, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London, SW7, 2AZ, United Kingdom. yulan.wang@imperial.ac.uk
authors.forename: Yulan
authors.initials: Y
authors.lastname: Wang
authors.forename: Huiru
authors.initials: H
authors.lastname: Tang
authors.forename: Elaine
authors.initials: E
authors.lastname: Holmes
authors.forename: John C
authors.initials: JC
authors.lastname: Lindon
authors.forename: Marco E
authors.initials: ME
authors.lastname: Turini
authors.forename: Norbert
authors.initials: N
authors.lastname: Sprenger
authors.forename: Gabriela
authors.initials: G
authors.lastname: Bergonzelli
authors.forename: Laurent B
authors.initials: LB
authors.lastname: Fay
authors.forename: Sunil
authors.initials: S
authors.lastname: Kochhar
authors.forename: Jeremy K
authors.initials: JK
authors.lastname: Nicholson
mesh_terms: D000367:Age Factors; D000818:Animals; D007421:Intestine, Small / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D008297:Male; D015999:Multivariate Analysis; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011522:Protons; D051381:Rats; D020318:Rats, Long-Evans
chemical_list: D011522:Protons