title: [Synthesis of purpure-18-imide and study on its interaction with bovine serum albumins by fluorescence].
abstract: Purpure-18-imide was synthesized based on the porphyrin's special affinity for cancer cells and the antitumor activity of pyrrolidine compound. The binding reaction between purpure-18-imide and bovine serum albumins (BSA) in aqueous was studied by fluorescence and UV-Vis absorption spectra. The research results indicated that the combination reaction of them was a single static quenching process. In aqueous, purpure-18-imide strongly bound BSA with molar ratio of 1:1. The binding constant K0 was 5.386 x 10(5) L x mol(-1). The shortest binding distance (r = 3.54 nm) and energy transfer efficiencies (E = 0.26) between donor (BSA) and acceptor (purpure-18-imide) were obtained by Forster's nonradiative energy transfer mechanism.
authors.affiliation: College of Chemistry, Yantai University, Yantai 264005, China.
authors.forename: Jin-jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Yong-ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000465:Algorithms; D000818:Animals; D002417:Cattle; D004735:Energy Transfer; D005453:Fluorescence; D007094:Imides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008956:Models, Chemical; D015394:Molecular Structure; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007094:Imides; D011166:Porphyrins; C549861:purpure-18-imide; C059051:purpurin 18; D012710:Serum Albumin, Bovine