Mouse mammary tumor virus Env-derived peptide associates with nucleolar targets in lymphoma, mammary carcinoma, and human breast cancer.
We have previously shown that the leader peptide (p14) of the Env-precursor of mouse mammary tumor virus is translocated into the nucleoli of murine T cell lymphomas that harbor this virus. Using a polyclonal antibody against recombinant p14, we show here that p14 is also localized to the nucleoli of murine mammary carcinomas and some human breast cancer samples. Affinity purification studies define a number of proteins, mostly nucleolar, that bind p14. Taken together, these findings point towards a more general involvement of p14 in lymphomagenesis and mammary carcinogenesis.
Bar-Sinai
Allan
A
Department of Cell and Animal Biology, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
Bassa
Nir
N
Fischette
Maria
M
Gottesman
Michael M
MM
Love
Dona C
DC
Hanover
John A
JA
Hochman
Jacob
J
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001943:Breast Neoplasms / Q000378:metabolism / Q000821:virology*; D002466:Cell Nucleolus / Q000378:metabolism / Q000821:virology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007150:Immunohistochemistry; D016399:Lymphoma, T-Cell / Q000378:metabolism / Q000821:virology*; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism* / Q000821:virology; D008324:Mammary Tumor Virus, Mouse / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins