Generation of reversible Rb-knockdown mice.
This study describes the generation of reversible Rb-knockdown mice using Tet-off system coupled with Rb-deficient mice currently available. Mice expressing pRB conditionally in Rb-/- background were generated by crossings P(hCMV)-tTA/TRE-Rb transgenic mice with conventional Rb+/- mice. Transgenic Rb was tightly controlled with reversibility and biologically effective as exemplified by cyclin E expression in a doxycycline-dependent manner in mouse embryonic fibroblasts. However, its ectopic expression was not sufficient to rescue the phenotypes of Rb-/- embryos at organismal level, suggesting the requirement of more sophisticated regulation of pRB. With all, these results demonstrate that our experimental strategy can be an alternative way to convert classical gene-disrupted mice into reversible conditional ones.
Choi
Yoon Sik
YS
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute, 300 Chonchon-Dong, Changan-Gu, Suwon 440-746, Republic of Korea.
Lee
Jae Eun
JE
Cheong
Cheolho
C
Sung
Young Hoon
YH
Yang
Eun Young
EY
Park
Chan Bae
CB
Song
Jaewhan
J
Park
Sang Chul
SC
Lee
Han-Woong
HW
D000818:Animals; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010641:Phenotype; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes
D016160:Retinoblastoma Protein