[Effects of benefiting-bone capsule on the expression of transforming growth factor-beta1 mRNA in bone tissue of the ovariectomized rats].
OBJECTIVE
To investigate the effects of Benefiting-bone capsule (BBC) on the expression of transforming growth factor-beta1 mRNA in bone tissue of the ovariectomized rats to further explain the mechanism of BBC on preventing and treating osteopoprosis.


METHODS
Twenty-four rats were randomly chosen from 72 female SD rats aged 10 months, which were classified into group A and B of sham operation dealing with normal physiological saline. The left 48 rats were randomly divided into 4 groups after ovariectomy: group A and B of osteoporotic animal model with normal physiological saline, prevented and treated groups of osteoporotic animal model with BBC. Each group included 12 rats. Ovariectomy was used to establish the osteoporotic animal model and the expression of TGF-beta1 mRNA on gene level in bone tissue was detected by real time fluorescence quantitative PCR (FQ-PCR) method.


RESULTS
The expression of TGF-beta1 mRNA on gene level in bone tissue of the groups of osteoporotic animal model with BBC were all higher than that of the groups of osteoporotic animal model, which were close to the groups of sham operation.


CONCLUSIONS
BBC can improve the expression of TGF-beta1 mRNA on gene level in bone tissue, which may be the mechanism of BBC on preventing and treating osteoporosis.
Zhang
Ronghua
R
The Affiliated First Hospital, Jinan University, Guangzhou 510632.
Yang
Li
L
Zhu
Xiaofeng
X
Cai
Yu
Y
Huang
Feng
F
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D002214:Capsules; D004195:Disease Models, Animal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D010024:Osteoporosis / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control; D010052:Ovariectomy; D010946:Plants, Medicinal / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D053773:Transforming Growth Factor beta1
D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1