title: Semiquantitative histomorphometric analysis of myocardium following partial left ventriculectomy: 1-year follow-up.
abstract: BACKGROUND
  Although partial left ventriculectomy (PLV) may have beneficial clinical effects in patients with dilated cardiomyopathy (DCM), there are no reports on effects of PLV on myocardial histology. The objective of this study was to assess histological properties of the LV myocardium 1 year following PLV as compared to histology at the time of the operation.
  
  
  METHODS
  The study group consisted of 15 consecutive PLV survivors, predominantly male (13/15), aged 45+/-12 years. Surgical specimens and endomyocardial biopsies, taken 12 months postoperatively, were processed routinely and stained with Masson-trichrome. The following morphometric parameters were assessed semiquantitavely: (1) degree of hypertrophy and attenuation; (2) nuclear evidence of hypertrophy; (3) myofibrillar volume fraction; (4) degree of degenerative vacuolar changes; and (5) fibrosis volume fraction.
  
  
  RESULTS
  Both New York Heart Association (NYHA) functional class and ejection fraction (EF) improved 12 months following surgery as compared to preoperative values (2.40+/-0.69 vs. 3.33+/-0.49, p<0.001, and 33.21+/-12.05% vs. 20.21+/-9.07%, p<0.001, respectively). Morphometric analysis demonstrated postoperative decrease in the degree of attenuation as compared to preoperative values (1.40+/-0.51 vs. 2.47+/-0.64, p<0.01), as well as a decrease in fibrosis volume fraction (2.07+/-0.80 vs. 2.67+/-0.49, p<0.001) and nuclear hypertrophy (1.27+/-0.46 vs. 1.67+/-0.62, p<0.05). On the other hand, postoperative increase in myofibrillar volume fraction (1.87+/-0.61 vs. 1.40+/-0.61, p<0.01) was noted.
  
  
  CONCLUSIONS
  One year postoperatively, PLV has favourable effects on myocardial morphology that parallels improvement in the patient's functional status and LV systolic function.
authors.affiliation: Institute of Pathology, Belgrade University School of Medicine, Dr. Subotića 1, 11000, Serbia and Montenegro. lole@beotel.yu
authors.forename: Jovan D
authors.initials: JD
authors.lastname: Vasiljević
authors.forename: Petar
authors.initials: P
authors.lastname: Otasević
authors.forename: Zoran B
authors.initials: ZB
authors.lastname: Popović
authors.forename: Aleksandar N
authors.initials: AN
authors.lastname: Nesković
authors.forename: Radoslav
authors.initials: R
authors.lastname: Vidaković
authors.forename: Zoran V
authors.initials: ZV
authors.lastname: Popović
authors.forename: Branislav
authors.initials: B
authors.lastname: Radovancević
authors.forename: O Howard
authors.initials: OH
authors.lastname: Frazier
authors.forename: Sinisa
authors.initials: S
authors.lastname: Gradinac
mesh_terms: D000328:Adult; D002311:Cardiomyopathy, Dilated / Q000503:physiopathology / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D006352:Heart Ventricles / Q000601:surgery*; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology*; D013318:Stroke Volume; D016277:Ventricular Function, Left