title: Establishment of an in vitro model using NR8383 cells and Mycobacterium bovis Calmette-Guerin that mimics a chronic infection of Mycobacterium tuberculosis.
abstract: BACKGROUND
  Mycobacterium tuberculosis infection affects one-third of the world's population and causes the death of three million people each year. To clarify details of M. tuberculosis survival strategies, it is important to establish a suitable in vitro model that mimics a chronic infection in alveolar macrophages by M. tuberculosis. For this reason, we established a new in vitro model using a rat alveolar macrophage cell line, NR8383.
  
  
  METHODS
  Basic characteristics, including phagocytotic ability and production of nitrogen oxide and tumor necrosis factor in response to several stimuli, of NR8383 cells were compared with those of primary alveolar macrophages. The course after phagocytosis of live or killed M. bovis bacilli Calmette-Guerin (BCG) was examined over 21 days using NR8383 cells as the host.
  
  
  RESULTS
  The characteristics that have been examined to date were nearly the same for both primary alveolar macrophage and NR8383 cells. Live BCG phagocytosed by NR8383 cells had successfully begun to grow in the cells within 7 days, while killed BCG were almost completely destroyed by 21 days.
  
  
  CONCLUSIONS
  BCG-infected NR8383 cells are potentially a suitable in vitro model that mimics a chronic infection with M tuberculosis.
authors.affiliation: Institute for Health Sciences, Tokushima Bunri University, Nishihama, Yamashiro-cho, Tokushima-shi, 770-8514, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Hino
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Oda
authors.forename: Aya
authors.initials: A
authors.lastname: Yoshida
authors.forename: Kazue
authors.initials: K
authors.lastname: Nakata
authors.forename: Chie
authors.initials: C
authors.lastname: Kohchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishizawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inagawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hori
authors.forename: Kimiko
authors.initials: K
authors.lastname: Makino
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Terada
authors.forename: Gen-Ichiro
authors.initials: G
authors.lastname: Soma
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D003609:Dactinomycin / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D008264:Macrophages; D016676:Macrophages, Alveolar / Q000166:cytology / Q000382:microbiology; D051379:Mice; D008954:Models, Biological; D009163:Mycobacterium bovis / Q000472:pathogenicity*; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity*; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014376:Tuberculosis / Q000382:microbiology / Q000473:pathology*; D014380:Tuberculosis, Bovine / Q000382:microbiology / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha; D003609:Dactinomycin; D009569:Nitric Oxide; C033233:actinomycin D1