Construction and enzymatic degradation of multilayered poly-l-lysine/DNA films.
The layer-by-layer (LbL) self-assembly of poly-l-lysine (PLL) and deoxyribonucleic acid (DNA) was used to construct the enzymatic biodegradable multilayered films. The LbL build up of DNA multilayers was monitored by UV-vis spectrometry, and atomic force microscopy (AFM). AFM, UV-vis spectrometry and fluorescence spectrometry measurements indicated that 90% of DNA within the films was released almost linearly under 5 U mL(-1)alpha-chymotrypsin in PBS at 37 degrees C in 35 h. TEM and zeta potential experiments revealed that the released DNA molecules were condensed into the slight positive complexes with size from 20 to several hundred nanometers. The well-structured, easy processed enzymatic biodegradable multilayered film may have great potential for gene applications in tissue engineering, medical implants, etc.
Ren
Kefeng
K
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Ji
Jian
J
Shen
Jiacong
J
D001672:Biocompatible Materials / Q000737:chemistry*; D001673:Biodegradation, Environmental; D002918:Chymotrypsin / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry*; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D008422:Materials Testing; D008567:Membranes, Artificial; D010316:Particle Size; D011107:Polylysine / Q000737:chemistry*
D001672:Biocompatible Materials; D004337:Drug Carriers; D008567:Membranes, Artificial; D011107:Polylysine; D004247:DNA; D002918:Chymotrypsin; C117608:alpha-chymotrypsin