[Study on the mechanism of oocyte aneuploidy by spectral karyotyping].
OBJECTIVE
To establish the method of detecting oocyte aneuploidy by spectral karyotyping (SKY).


METHODS
The unfertilized oocytes were fixed 1-2 days after oocyte retrieval. Spectral karyotyping was performed according to the protocol.


RESULTS
64% of oocytes were normal, 36% of oocytes were aneuploidy, of which 22% were due to nondisjunction and 14% unbalanced predivision.


CONCLUSIONS
SKY is an effective method for detecting oocyte aneuploidy. Both nondisjunction and unbalanced predivision are involved in oocyte aneuploidy formation.
Chen
Wen
W
Department of Gynecology and Obstetrics, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030 PR China. chenwenyyy@yahoo.com
Zhu
Gui-jin
GJ
Mettler
Liselotte
L
D000782:Aneuploidy; D005260:Female; D006801:Humans; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D015203:Reproducibility of Results; D032681:Spectral Karyotyping / Q000379:methods*