title: Primers for clinical detection of Paracoccidioides brasiliensis.
abstract: From a 0.72-kb fragment universally generated in Paracoccidioides brasiliensis strains, primers were designed and tested on genomic DNA of this and other pathogenic fungi. They were specific and highly sensitive for P. brasiliensis DNA. Positive results were obtained when these were tested in clinical samples.
authors.affiliation: Instituto Venezolano de Investigaciones Científicas, Centro de Microbiología y Biología Celular, Apartado 21827, Caracas 1020A, Venezuela. sanblasg@ivic.ve
authors.forename: Gioconda
authors.initials: G
authors.lastname: San-Blas
authors.forename: Gustavo
authors.initials: G
authors.lastname: Niño-Vega
authors.forename: Laura
authors.initials: L
authors.lastname: Barreto
authors.forename: Flavia
authors.initials: F
authors.lastname: Hebeler-Barbosa
authors.forename: Eduardo
authors.initials: E
authors.lastname: Bagagli
authors.forename: Rosa
authors.initials: R
authors.lastname: Olivero de Briceño
authors.forename: Rinaldo Poncio
authors.initials: RP
authors.lastname: Mendes
mesh_terms: D000328:Adult; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010228:Paracoccidioides / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal