Protein interaction of nucleosome assembly protein 1 and casein kinase 2 during desiccation response in the insect-killing nematode Steinernema feltiae IS-6.
The change in gene expression induced by desiccation in the semiarid, entomopathogenic nematode Steinernema feltiae IS-6, includes induction of transcription of a nucleosome assembly protein, NAP1 homolog, and of casein kinase 2 (CK2) genes. Therefore, one of the events during the dehydration response of S. feltiae IS-6 may be transcriptional activation by S. feltiae IS-6 NAP1 homolog (Sf-Nap1), which is regulated by S. feltiae IS-6 CK2 (Sf-CK2). This regulation necessitates physical interaction between the Sf-Nap1 and Sf-CK2 proteins. In the present study we used yeast 2-hybrid analysis to demonstrate physical interaction between the 2 proteins, thus confirming the involvement of a protein interaction-based step in the desiccation response mechanism of S. feltiae IS-6.
Gal
T Z
TZ
Department of Nematology, ARO, The Volcani Center, Bet Dagan 50250, Israel.
Glazer
I
I
Sherman
A
A
Koltai
H
H
D000818:Animals; D047390:Casein Kinase II / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D003890:Desiccation; D015870:Gene Expression / Q000502:physiology; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D056490:Nucleosome Assembly Protein 1; D009707:Nucleosomes / Q000502:physiology; D010572:Pest Control, Biological; D017168:Rhabditida / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014170:Transformation, Genetic / Q000502:physiology
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D056490:Nucleosome Assembly Protein 1; D009707:Nucleosomes; D047390:Casein Kinase II