Histologic evaluation of the effect of nicotine administration on bone regeneration. A study in dogs.
The objective of this study was to investigate the histometric impact of nicotine on bone regeneration of surgically created alveolar ridge defects in dogs. Sixteen mongrel dogs were used. One defect was surgically created unilaterally in the mandible, and left to heal spontaneously. The animals were randomly assigned to one of the following groups: Group 1--control (n = 8) and Group 2--subcutaneous nicotine administration (2 mg/kg) twice a day (n = 8). After 4 months, the animals were sacrificed and the specimens routinely processed for semi-serial decalcified sections. Bone height (BH), bone width (BW), bone density (BD), and bone area (BA) of the newly-formed bone were evaluated. Intergroup analysis (Mann-Whitney rank sum test) showed that regardless of the presence of nicotine, no significant differences were observed regarding bone width (BW), bone area (BA) and bone height (BH) (p > 0.05). On the other hand, it was demonstrated that nicotine administration significantly influenced the proportion of mineralized tissue within the limits of the newly-formed bone (BD) (p < 0.001). Within the limits of the present study, it can be concluded that nicotine might affect but not prevent bone healing in defects left to heal spontaneously.
Saldanha
Juliana Bezerra
JB
Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry of Piracicaba, State University of Campinas.
Pimentel
Suzana Peres
SP
Casati
Marcio Zaffalon
MZ
Sallum
Antonio Wilson
AW
Sallum
Enilson Antonio
EA
Nociti
Francisco Humberto Júnior
FH
D000539:Alveolar Process / Q000187:drug effects* / Q000601:surgery; D000541:Alveolectomy; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D003367:Cotinine / Q000097:blood; D004285:Dogs; D023421:Models, Animal; D009538:Nicotine / Q000097:blood / Q000494:pharmacology*; D011897:Random Allocation; D018709:Statistics, Nonparametric; D014945:Wound Healing / Q000187:drug effects
D009538:Nicotine; D003367:Cotinine