title: Surface properties of sulfur- and ether-linked phosphonolipids with and without purified hydrophobic lung surfactant proteins.
abstract: Two novel C16:0 sulfur-linked phosphonolipids (S-lipid and SO(2)-lipid) and two ether-linked phosphonolipids (C16:0 DEPN-8 and C16:1 UnDEPN-8) were studied for surface behavior alone and in mixtures with purified bovine lung surfactant proteins (SP)-B and/or SP-C. Synthetic C16:0 phosphonolipids all had improved adsorption and film respreading compared to dipalmitoyl phosphatidylcholine, and SO(2)-lipid and DEPN-8 reached maximum surface pressures of 72mN/m (minimum surface tensions of <1mN/m) in compressed films on the Wilhelmy balance (23 degrees C). Dispersions of DEPN-8 (0.5mg/ml) and SO(2)-lipid (2.5mg/ml) also reached minimum surface tensions of <1mN/m on a pulsating bubble surfactometer (37 degrees C, 20cycles/min, 50% area compression). Synthetic lung surfactants containing DEPN-8 or SO(2)-lipid+0.75% SP-B+0.75% SP-C had dynamic surface activity on the bubble equal to that of calf lung surfactant extract (CLSE). Surfactants containing DEPN-8 or SO(2)-lipid plus 1.5% SP-B also had very high surface activity, but less than when both apoproteins were present together. Adding 10wt.% of UnDEPN-8 to synthetic lung surfactants did not improve dynamic surface activity. Surfactants containing DEPN-8 or SO(2)-lipid plus 0.75% SP-B/0.75% SP-C were chemically and biophysically resistant to phospholipase A(2) (PLA(2)), while CLSE was severely inhibited by PLA(2). The high activity and inhibition resistance of synthetic surfactants containing DEPN-8 or SO(2)-lipid plus SP-B/SP-C are promising for future applications in treating surfactant dysfunction in inflammatory lung injury.
authors.affiliation: Department of Pediatrics, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA.
authors.forename: Yusuo
authors.initials: Y
authors.lastname: Chang
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Wang
authors.forename: Adrian L
authors.initials: AL
authors.lastname: Schwan
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Wang
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Holm
authors.forename: John E
authors.initials: JE
authors.lastname: Baatz
authors.forename: Robert H
authors.initials: RH
authors.lastname: Notter
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D004987:Ethers / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D010741:Phospholipases A / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D037701:Pulmonary Surfactant-Associated Protein B / Q000737:chemistry*; D037721:Pulmonary Surfactant-Associated Protein C / Q000737:chemistry*; D013450:Sulfones / Q000737:chemistry*; D013500:Surface Tension; D013816:Thermodynamics
chemical_list: D004987:Ethers; D010743:Phospholipids; D037701:Pulmonary Surfactant-Associated Protein B; D037721:Pulmonary Surfactant-Associated Protein C; D013450:Sulfones; C028299:phosphonolipids; D010741:Phospholipases A