title: OpsX from Haemophilus influenzae represents a novel type of heptosyltransferase I in lipopolysaccharide biosynthesis.
abstract: The inner core region of the lipopolysaccharide (LPS) of Haemophilus influenzae is characterized by the presence of a phosphorylated 3-deoxy-alpha-D-manno-octulosonic acid (Kdo). In this study, we show that the heptosyltransferase I adding the first L-glycero-D-manno-heptose residue to this acceptor is encoded by the gene opsX, which differs in substrate specificity from the other heptosyltransferase I, known as WaaC.
authors.affiliation: Division of Medical and Biochemical Microbiology, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 22, D-23845 Borstel, Germany. sgronow@fz-borstel.de
authors.forename: Sabine
authors.initials: S
authors.lastname: Gronow
authors.forename: Werner
authors.initials: W
authors.lastname: Brabetz
authors.forename: Buko
authors.initials: B
authors.lastname: Lindner
authors.forename: Helmut
authors.initials: H
authors.lastname: Brade
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016695:Glycosyltransferases / Q000378:metabolism*; D006193:Haemophilus influenzae / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D013058:Mass Spectrometry; D010957:Plasmids; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D016695:Glycosyltransferases; C504964:OpsX protein, Haemophilus influenzae