Ultrastructural effects of antimicrobial peptides from bovine lactoferrin on the membranes of Candida albicans and Escherichia coli.
Antimicrobial peptides allegedly exert their action on microbial membranes. Bovine lactoferrin enfold two antimicrobial domains, lactoferricin B (LFcin B) and lactoferrampin (LFampin). Effects of representative peptides thereof on the membranes of Candida albicans and Escherichia coli were investigated. Confocal laser scanning microscopy revealed that these peptides were internalized within a few minutes, concurrently with disrupting membrane integrity as indicated by freeze-fracture transmission electron microscopy. The most striking findings were induction of distinct vesicle-like structures in the membrane of C. albicans by the LFampin peptide, and detachment of the outer membrane and surface protrusions in E. coli by the LFcin B peptide.
van der Kraan
Marieke I A
MI
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit and Universiteit van Amsterdam, Van der Boechorststraat 7, 1081BT Amsterdam, The Netherlands.
van Marle
Jan
J
Nazmi
Kamran
K
Groenink
Jasper
J
van 't Hof
Wim
W
Veerman
Enno C I
EC
Bolscher
Jan G M
JG
Nieuw Amerongen
Arie V
AV
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects* / Q000648:ultrastructure; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005614:Freeze Fracturing; D007781:Lactoferrin / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry; D018613:Microscopy, Confocal; D008854:Microscopy, Electron / Q000379:methods; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology
D023181:Antimicrobial Cationic Peptides; D007782:Lactoglobulins; D010446:Peptide Fragments; C502040:lactoferrampin; C082171:lactoferricin B; D007781:Lactoferrin; D016650:Fluorescein-5-isothiocyanate