title: Effect of cytokines on ICAM-1 and ZO-1 expression on human airway epithelial cells.
abstract: The presence of adhesion molecules on airway epithelial cells may be important in recruiting leukocytes to the epithelium. The study aimed at investigating the effects of interleukin (IL)-4, IL-8, IL-13 and interferon-gamma (IFN-gamma) on cell viability and intracellular adhesion molecule (ICAM)-1 and zonula occludens protein (ZO)-1 expression on cultured human basal and columnar airway epithelial cells. Cycloheximide (CHX) induced cell death in both cell lines. The cytokines IL-4, IL-8, IL-13 and IFN-gamma had only minor effects on cell proliferation in the columnar 16HBE14o-cells, and inhibited the effects of CHX on cell death. IFN-gamma increased ICAM-1 expression in both cell lines. Western blot analysis showed that CHX inhibited both ICAM-1 and ZO-1 expression in the basal cell line. A combination of IL-4 and IFN-gamma appeared to break the tight junctions. IL-4 and IL-13 potentiated CHX-induced apoptosis in basal cells but not in columnar cells, possibly due to low levels of the IL-4 receptor. It is concluded that cytokines produced by airway epithelium may have a role in regulating sequestering of leukocytes to the airways during airway inflammation.
authors.affiliation: Department of Medical Cell Biology, University of Uppsala, Box 571, SE-75 123 Uppsala, Sweden.
authors.forename: Anne-Jacqueline
authors.initials: AJ
authors.lastname: Relova
authors.forename: Shahida
authors.initials: S
authors.lastname: Shahana
authors.forename: Natalia
authors.initials: N
authors.lastname: Makeeva
authors.forename: Godfried M
authors.initials: GM
authors.lastname: Roomans
mesh_terms: D001980:Bronchi / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D016207:Cytokines / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000276:immunology; D007371:Interferon-gamma / Q000502:physiology; D018793:Interleukin-13 / Q000502:physiology; D015847:Interleukin-4 / Q000502:physiology; D007962:Leukocytes / Q000502:physiology; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000276:immunology; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000276:immunology; D019948:Receptors, Interleukin-4 / Q000096:biosynthesis; D020545:Respiratory Mucosa / Q000276:immunology / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D019108:Tight Junctions / Q000502:physiology / Q000648:ultrastructure; D062826:Zonula Occludens-1 Protein
chemical_list: D016207:Cytokines; D018793:Interleukin-13; D008565:Membrane Proteins; D010750:Phosphoproteins; D019948:Receptors, Interleukin-4; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D018799:Intercellular Adhesion Molecule-1; D015847:Interleukin-4; D007371:Interferon-gamma