title: Induced accumulation of oleanolic acid and ursolic acid in cell suspension cultures of Uncaria tomentosa.
abstract: Increasing sucrose from 20 to 50 g l(-1) in Uncaria tomentosa cell suspension cultures enhanced ursolic acid and oleanolic acid production from 129 +/- 61 to 553 +/- 193 microg g(-1) cell dry wt. The maximal concentration of both triterpenes (1680 +/- 39 microg g(-1) cell dry wt) was 8 days after elicitation by jasmonic acid, while yeast extract or citrus pectin treatments produced 1189 +/- 20 or 1120 +/- 26 microg g(-1) cell dry wt, respectively. The ratio of ursolic acid:oleanolic acid was constant at 70:30.
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Av. Instituto Politécnico Nacional, México.
authors.forename: Iris
authors.initials: I
authors.lastname: Feria-Romero
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Lazo
authors.forename: Teresa
authors.initials: T
authors.lastname: Ponce-Noyola
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Cerda-García-Rojas
authors.forename: Ana C
authors.initials: AC
authors.lastname: Ramos-Valdivia
mesh_terms: D018533:Biomass; D020948:Cat's Claw / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000737:chemistry / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors; D014315:Triterpenes / Q000737:chemistry / Q000378:metabolism*; D000097245:Ursolic Acid
chemical_list: D009828:Oleanolic Acid; D013395:Sucrose; D014315:Triterpenes; D000097245:Ursolic Acid