Activation of monoamine oxidase isotypes by prolonged intake of aluminum in rat brain.
Rats were fed 100 microM aluminum maltolate for one year in their drinking water. Brain aluminum contents have increased 4.2-fold in the aluminum-treated group, whereas no significant changes in the body weight, brain weight, and brain protein content were observed. Long-term aluminum feeding induced apoptosis as assessed by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method and showed activatory effects on the catalytic efficiency (kcat/KM) of monoamine oxidase-A and monoamine oxidase-B up to 1.9- and 3.8-fold, respectively. The expression level of monoamine oxidase isotypes on the Western blot remained unchanged between the two groups, suggesting a change in post-translational regulation of the activities of monoamine oxidase isotypes by long-term aluminum feeding.
Huh
Jae-Wan
JW
Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, 388-1 Poongnap-dong, Songpa-gu, Seoul 138-736, Republic of Korea.
Choi
Myung-Min
MM
Lee
Jang Han
JH
Yang
Seung-Ju
SJ
Kim
Mi Jung
MJ
Choi
Jene
J
Lee
Kwan Ho
KH
Lee
Jong Eun
JE
Cho
Sung-Woo
SW
D000284:Administration, Oral; D000535:Aluminum / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology; D004789:Enzyme Activation; D020869:Gene Expression Profiling; D008297:Male; D008995:Monoamine Oxidase / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000535:Aluminum; D008995:Monoamine Oxidase