title: A new recombinant virus system for the study of HIV-1 entry and inhibition.
abstract: The construction is described of a HIV-1 proviral, eGFP-tagged plasmid that allows for the recombination of any selected env gene without the use of restriction enzymes and for the quantitation of the infection by the recombinant virus using flow cytometry. The system was tested showing that an isoleucine to valine substitution at residue position 37 of the HIV-1 gp41 impairs the fitness of the virus but does not lead by itself to T-20 resistance.
authors.affiliation: Retrovirology Laboratory, Centre de Recherche-Public (CRP)-Santé, 84 rue Val Fleuri, L-1526 Luxembourg, Luxembourg. roman.f@retrovirology.lu
authors.forename: Francois
authors.initials: F
authors.lastname: Roman
authors.forename: Wim
authors.initials: W
authors.lastname: Ammerlaan
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Plesseria
authors.forename: Sabrina
authors.initials: S
authors.lastname: Deroo
authors.forename: Claude P
authors.initials: CP
authors.lastname: Muller
authors.forename: Vic
authors.initials: V
authors.lastname: Arendt
authors.forename: Francois
authors.initials: F
authors.lastname: Schneider
authors.forename: Robert
authors.initials: R
authors.lastname: Hemmer
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Schmit
mesh_terms: D019943:Amino Acid Substitution; D003001:Cloning, Molecular; D024882:Drug Resistance, Viral; D000077560:Enfuvirtide; D005434:Flow Cytometry; D015700:HIV Envelope Protein gp41 / Q000235:genetics / Q000494:pharmacology; D023581:HIV Fusion Inhibitors / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology; D011533:Proviruses / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
chemical_list: D015700:HIV Envelope Protein gp41; D023581:HIV Fusion Inhibitors; D010446:Peptide Fragments; D000077560:Enfuvirtide