title: The human platelet membrane proteome reveals several new potential membrane proteins.
abstract: We present the first focused proteome study on human platelet membranes. Due to the removal of highly abundant cytoskeletal proteins a wide spectrum of known platelet membrane proteins and several new and hypothetical proteins were accessible. In contrast to other proteome studies we focused on prefractionation and purification of membranes from human platelets according to published protocols to reduce sample complexity and enrich interesting membrane proteins. Subsequently protein separation by common one-dimensional SDS-PAGE as well as the combined benzyldimethyl-n-hexadecylammonium chloride/SDS separation technique was performed prior to mass spectrometry analysis by nano-LC-ESI-MS/MS. We demonstrate that the application of both separation systems in parallel is required for maximization of protein tagging out of a complex sample. Furthermore the identification of several potential membrane proteins in human platelets yields new potential targets in functional platelet research.
authors.affiliation: Protein Mass Spectrometry and Functional Proteomics Group, Rudolf Virchow Center for Experimental Biomedicine, Julius Maximilians University of Wuerzburg, Versbacher Strasse 9, 97078 Wuerzburg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Moebius
authors.forename: René Peiman
authors.initials: RP
authors.lastname: Zahedi
authors.forename: Urs
authors.initials: U
authors.lastname: Lewandrowski
authors.forename: Claudia
authors.initials: C
authors.lastname: Berger
authors.forename: Ulrich
authors.initials: U
authors.lastname: Walter
authors.forename: Albert
authors.initials: A
authors.lastname: Sickmann
mesh_terms: D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D008565:Membrane Proteins; D020543:Proteome