title: Quantitative analysis of membrane proteins from breast cancer cell lines BT474 and MCF7 using multistep solid phase mass tagging and 2D LC/MS.
abstract: We introduce a new multistep mass tagging technique and show its utility for reducing sample complexity when coupled with two-dimensional liquid chromatography/nano-electrospray ionization ion trap mass spectrometry (2D LC/nano ESI-MS). Solid-phase mass tagging reagents were used to identify and obtain relative quantitation of membrane proteins from two established breast cancer cell lines, BT474 and MCF7. The results presented in this study show that sample complexity can be reduced with corresponding increases in protein identification and quantitation.
authors.affiliation: Department of Chemical Engineering, Yale University, New Haven, CT 06520-8286, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
authors.forename: Rong
authors.initials: R
authors.lastname: Xiang
authors.forename: Csaba
authors.initials: C
authors.lastname: Horváth
authors.forename: James A
authors.initials: JA
authors.lastname: Wilkins
mesh_terms: D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000737:chemistry*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry / Q000235:genetics; D015203:Reproducibility of Results
chemical_list: D008565:Membrane Proteins; D009363:Neoplasm Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases