title: Occurrence of pS86/pEF47-related plasmids in gram-positive cocci.
abstract: A small, low-copy-number cryptic 5.5-kb plasmid, designated pEF47, was isolated from a rumen bacterium Enterococcus faecalis 47 and partially characterized. Comparisons by restriction and partial sequence analysis of pEF47 demonstrated high homology to pS86 plasmid, recently isolated from a human clinical strain E. faecalis S-86. PCR followed by sequence analysis and Southern hybridization showed that pS86/pEF47-related replicons are regularly encountered in plasmids from gram-positive cocci.
authors.affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Soltesovej 4-6, Kosice, 040 01, Slovakia.
authors.forename: Adriana
authors.initials: A
authors.lastname: Sprincova
authors.forename: Viliam
authors.initials: V
authors.lastname: Stovcik
authors.forename: Peter
authors.initials: P
authors.lastname: Javorsky
authors.forename: Peter
authors.initials: P
authors.lastname: Pristas
mesh_terms: D000818:Animals; D013293:Enterococcus faecalis / Q000235:genetics; D006095:Gram-Positive Cocci / Q000235:genetics*; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification*; D012093:Replicon / Q000235:genetics; D012417:Rumen / Q000382:microbiology; D012689:Sequence Homology, Nucleic Acid