The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: target proteins identified.
Infection of Escherichia coli by bacteriophage T4 leads to the expression of three phage mono-ADP-ribosyltransferases (namely, Alt, ModA, and ModB), each of which modifies a distinct group of host proteins. To improve understanding of these interactions and their consequences for the T4 replication cycle, we used high-resolution two-dimensional gel electrophoresis and mass-spectrometry to identify some of the putative target proteins ADP-ribosylated in vitro by Alt (total approximately 27) and ModB (total approximately 8). E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication.
Depping
Reinhard
R
AG Molekulare Genetik, Ruhr-Universität Bochum, Bochum, Germany. Depping@physio.uni-luebeck.de
Lohaus
Christiane
C
Meyer
Helmut E
HE
Rüger
Wolfgang
W
D036002:ADP Ribose Transferases / Q000378:metabolism*; D017122:Bacteriophage T4 / Q000378:metabolism*; D001665:Binding Sites; D016503:Drug Delivery Systems / Q000379:methods; D004926:Escherichia coli / Q000821:virology*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D010444:Peptide Elongation Factor Tu / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D014779:Virus Replication / Q000502:physiology*
D029968:Escherichia coli Proteins; D036002:ADP Ribose Transferases; D010444:Peptide Elongation Factor Tu; C403324:trigger factor, E coli; D019696:Peptidylprolyl Isomerase