Ultrasmall superparamagnetic iron-oxide-enhanced MR imaging of normal bone marrow in rodents: original research original research.
OBJECTIVE
The objective is to compare three different ultrasmall superparamagnetic iron oxides (USPIOs) for magnetic resonance (MR) imaging of normal bone marrow in rodents.


METHODS
Femoral bone marrow in 18 Sprague-Dawley rats was examined by using MR imaging before and up to 2 and 24 hours postinjection (PI) of 200 mumol of Fe/kg of SHU555C (n = 6), ferumoxtran-10 (n = 6), or ferumoxytol (n = 6), using T1-weighted (50 ms/1.7 ms/60 degrees = repetition time [TR]/echo time [TE]/flip angle) and T2*-weighted (100 ms/15 ms/38 degrees = TR/TE/flip angle) three-dimensional spoiled gradient recalled echo sequences. USPIO-induced bone marrow was evaluated qualitatively and quantified as signal-to-noise ratio (SNR) and change in signal intensity (DeltaSI) values. A mixed-effect model was fitted to the SNR and DeltaSI values, and differences among USPIOs were tested for significance by using F tests.


RESULTS
At 2 hours PI, all three USPIOs showed marked positive signal enhancement on T1-weighted images and a corresponding marked signal loss on T2*-weighted images. At 24 hours PI, the T1 effect of all three USPIOs disappeared, whereas T2*-weighted images showed persistent signal loss on SHU555C and ferumoxytol-enhanced MR images, but not ferumoxtran-10-enhanced MR images. Corresponding SNR and DeltaSI values on T2*-weighted MR images at 24 hours PI were significantly different from baseline for SHU555C and ferumoxytol, but not ferumoxtran-10.


CONCLUSIONS
All three USPIO contrast agents, ferumoxtran-10, ferumoxytol, and SHU555C, can be applied for MR imaging of bone marrow. Ferumoxtran-10 apparently reveals a different kinetic behavior in bone marrow than ferumoxytol and SHU555C.
Simon
Gerhard H
GH
University of California at San Francisco Medical Center, Department of Radiology, S-372, Center for Pharmaceutical and Molecular Imaging, 513 Parnassus Avenue, San Francisco, CA 94143, USA. gsimon@radiology.ucsf.edu
Raatschen
Hans-Juergen
HJ
Wendland
Michael F
MF
von Vopelius-Feldt
Johannes
J
Fu
Yanjun
Y
Chen
Mei-Hsiu
MH
Daldrup-Link
Heike E
HE
D000818:Animals; D001853:Bone Marrow / Q000033:anatomy & histology*; D003287:Contrast Media / Q000493:pharmacokinetics*; D003911:Dextrans; D005260:Female; D005269:Femur; D052203:Ferrosoferric Oxide / Q000493:pharmacokinetics*; D021621:Imaging, Three-Dimensional; D007501:Iron / Q000493:pharmacokinetics*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D010087:Oxides / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; C097921:ferumoxtran-10; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide