title: Separation and determination of clotrimazole, methylparaben and propylparaben in pharmaceutical preparation by micellar electrokinetic chromatography.
abstract: In this study, micellar electrokinetic chromatography (MEKC) method was developed for the determination of clotrimazole (CLO), methylparaben (MP) and propylparaben (PP) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (60 cm x 75 microm i.d.) at 25 kV with UV detection at 212 nm. Optimized background electrolyte (BGE) was 15 mM phosphate buffer (pH 7.2) containing 30 mM sodium dodecyl sulfate (SDS) as a surfactant. Rectilinear calibration ranges were 50-500 mg l(-1) for CLO, 10-100 mg l(-1) for MP and 2.5-25 mg l(-1) for PP. The total analysis time was < 12 min.
authors.affiliation: Charles University, Faculty of Pharmacy, Department of Analytical Chemistry, Heyrovského 1203, CZ-500 05 Hradec Králové, Czech Republic.
authors.forename: Rafífa
authors.initials: R
authors.lastname: Hamoudová
authors.forename: Marie
authors.initials: M
authors.lastname: Pospísilová
authors.forename: Andrea
authors.initials: A
authors.lastname: Kavalírová
authors.forename: Petr
authors.initials: P
authors.lastname: Solich
authors.forename: Jan
authors.initials: J
authors.lastname: Sícha
mesh_terms: D000891:Anti-Infective Agents, Local / Q000032:analysis / Q000737:chemistry; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003022:Clotrimazole / Q000032:analysis* / Q000737:chemistry; D004573:Electrolytes; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010226:Parabens / Q000032:analysis* / Q000737:chemistry; D010710:Phosphates / Q000032:analysis; D011310:Preservatives, Pharmaceutical / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000032:analysis; D013696:Temperature; D013997:Time Factors
chemical_list: D000891:Anti-Infective Agents, Local; D004573:Electrolytes; D010226:Parabens; D010710:Phosphates; D011310:Preservatives, Pharmaceutical; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C015358:methylparaben; D003022:Clotrimazole; C006068:propylparaben