Generation of embryoid bodies from mouse embryonic stem cells cultured on STO feeder cells.
Embryoid bodies, which are similar to post-implantation egg-cylinder stage embryos, provide a model for the study of embryo development and stem cell differentiation. We describe here a novel method for generating embryoid bodies from murine embryonic stem (ES) cells cultured on the STO feeder layer. The ES cells grew into compact aggregates in the first 3 days of coculture, then became simple embryoid bodies (EBs) possessing primitive endoderm on the outer layer. They finally turned into cystic embryoid bodies after being transferred to Petri dishes for 1-3 days. Evaluation of the EBs in terms of morphology and differentiating potential indicates that they were typical in structure and could generate cells derived from the three germ layers. The results show that embryoid bodies can form not only in suspension culture but also directly from ES cells cultured on the STO feeder layer.
Zhou
Qing-Jun
QJ
College of Life Science, Zhejiang University, Hangzhou, Zhejiang 310012, PR China.
Shao
Jian-Zhong
JZ
Xiang
Li-Xin
LX
Hu
Ruo-Zhen
RZ
Lu
Yong-Liang
YL
Yao
Hang
H
Dai
Li-Cheng
LC
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D002449:Cell Aggregation; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D019070:Cell Lineage; D018920:Coculture Techniques / Q000379:methods*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D050991:GATA4 Transcription Factor / Q000032:analysis / Q000235:genetics; D005855:Germ Layers / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D022781:Hepatocytes / Q000737:chemistry / Q000166:cytology; D051379:Mice; D046529:Microscopy, Electron, Transmission; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000166:cytology; D016900:Neurofilament Proteins / Q000032:analysis / Q000235:genetics; D009474:Neurons / Q000737:chemistry / Q000166:cytology; D012333:RNA, Messenger / Q000032:analysis; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D014212:Tretinoin / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000032:analysis / Q000235:genetics
D002087:Butyrates; D050991:GATA4 Transcription Factor; C484171:Gata4 protein, mouse; D016900:Neurofilament Proteins; D012333:RNA, Messenger; D000509:alpha-Fetoproteins; D014212:Tretinoin; D004121:Dimethyl Sulfoxide