Fixation of microsporidian spores for electron microscopy.
Fresh and frozen spores of the microsporidia Nosema apis and Nosema bombi were fixed using various fixatives at different times and temperatures. Paraformaldehyde and technical formaldehyde gave results comparable to or better than glutaraldehyde. Increased fixation temperature improved the fixation of spores from terrestrial hosts. Freezing did not destroy the cytology of the spore.
Larsson
J I Ronny
JI
Department of Cell and Organism Biology, University of Lund, Helgonavagen 3, S-223 62 Lund, Sweden. ronny.larsson@cob.lu.se
D008854:Microscopy, Electron; D016814:Microsporidia / Q000648:ultrastructure*; D013170:Spores / Q000648:ultrastructure*; D016707:Tissue Fixation