Mucosal and systemic immunization elicited by Newcastle disease virus (NDV) transgenic plants as antigens.
As a step towards developing a safe and effective edible vaccine against Newcastle disease virus (NDV), we have explored the use of plants genetically engineered to express viral proteins. We report the construction of transgenic potato plants expressing the genes coding for immunogenic proteins of NDV under the regulation of CaMV 35S promoter and its immunogenicity in mice. All mice receiving transgenic plant extracts in incomplete Freund adjuvant produced specific anti-NDV antibodies. Animals fed with transgenic leaves showed a specific response against NDV. Detection of IgA released from in vitro-cultured intestinal tissue fragments indicated the presence of IgA-secreting cells in the gut.
Berinstein
Analia
A
Instituto de Biotecnología, CICVyA, INTA, Cc25 B1712WAA, Castelar, Buenos Aires, Argentina. aberinst@cicv.inta.gov.ar
Vazquez-Rovere
Cecilia
C
Asurmendi
Sebastian
S
Gómez
Evangelina
E
Zanetti
Flavia
F
Zabal
Osvaldo
O
Tozzini
Alejandro
A
Conte Grand
Daniela
D
Taboga
Oscar
O
Calamante
Gabriela
G
Barrios
Hebe
H
Hopp
Esteban
E
Carrillo
Elisa
E
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000917:Antibody Formation; D018928:Immunity, Mucosal / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009521:Newcastle Disease / Q000276:immunology* / Q000517:prevention & control; D009522:Newcastle disease virus / Q000235:genetics* / Q000276:immunology; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D014765:Viral Vaccines