Evidence of diversity within the SnRK1b gene family of Hordeum species.
Adaptor-specific polymerase chain reaction (PCR) was used to amplify 3 different products, termed variant A, B, and C, from the seed-specific class (SnRK1b) of the sucrose nonfermenting-1-related protein kinase gene family (SnRK1) of different Hordeum species and cultivars of barley (Hordeum vulgare). Standard PCR or reverse transcription-PCR (RT-PCR) at a high temperature, using primers that differed by 1 or 2 nucleotides, was then used to amplify and clone 3 specific variants. One primer pair amplified a variant from I genome species suggesting that this could be a useful I-genome specific marker. The corresponding genes of the 3 variants (A, B, and C) were termed SnRK1b.1, 2, and 3, respectively. SnRK1b.1 and 2, showed 98% - 100% nucleotide sequence identity in the coding region, and 89% - 90% identity in the promoter region (up to 200 bp upstream of the translation start site, ATG). However, they differed in having insertions, deletions, and base pair changes at potentially important sites in the polymerase binding regions. SnRK1b.3 showed 90% nucleotide sequence identity with SnRK1b.1 in the coding region and 86% in the promoter region. This gene predominates in H-genome species within the genus Hordeum and could be a useful marker for this group.
Clark
Jeremy S C
JS
Long Ashton Research Station, Bristol, UK.
Dani
Maria
M
Halford
Nigel G
NG
Karp
Angela
A
D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D018745:Genome, Plant; D001467:Hordeum / Q000201:enzymology / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012639:Seeds / Q000201:enzymology / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D018076:DNA, Complementary; D018744:DNA, Plant; D007527:Isoenzymes; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases