title: Phosphoenolpyruvate carboxykinase is an acid-induced, chromosomally encoded virulence factor in Agrobacterium tumefaciens.
abstract: The pckA gene, encoding phosphoenolpyruvate carboxykinase, catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate to form phosphoenolpyruvate. Located on the circular chromosome of Agrobacterium, this locus is adjacent to the loci chvG and chvI, encoding a two-component regulatory system that has been shown to be important in virulence. Using a reporter gene fusion, studies showed that the pckA gene is induced by acidic pH but not by acetosyringone. This acid induction is regulated by the chvG-chvI regulatory system, which controls acid-inducible genes. A pckA mutant had no demonstrable PckA enzyme activity and grew on AB minimal medium with glucose but did not grow on the same medium with succinate as the sole carbon source and was more inhibited in its growth than the wild-type strain by an acidic environment. A pckA mutant was highly attenuated in tumor-inducing ability on tobacco leaf disks and was severely attenuated in vir gene expression. Although vir gene induction was completely restored when a constitutive virG gene was introduced into the mutant strain, virulence was only partially restored. These results suggest that avirulence may be due to a combination of the inhibition of this mutant in the acidic plant wound environment and the poor induction of the vir genes.
authors.affiliation: Department of Microbiology, Box 357242, University of Washington, Seattle, WA 98195-7242, USA.
authors.forename: Pu
authors.initials: P
authors.lastname: Liu
authors.forename: Derek
authors.initials: D
authors.lastname: Wood
authors.forename: Eugene W
authors.initials: EW
authors.lastname: Nester
mesh_terms: D016960:Agrobacterium tumefaciens / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003470:Culture Media; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D005816:Genetic Complementation Test; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation; D014774:Virulence / Q000235:genetics*
chemical_list: D003470:Culture Media; D017931:DNA Primers; D019764:Phosphoenolpyruvate Carboxykinase (ATP)