Evaluation of radioprotective effect of vitamin E in salivary dysfunction in irradiated rats.
The aim of this study was to evaluate the radioprotective effect of vitamin E in salivary gland function, as well as analyse the total protein concentration. For this purpose 90 male rats were used and randomly divided into five experimental groups: control (I), in which animals received olive oil solution but were not irradiated; irradiated-olive oil (II), in which animals received olive oil solution and were irradiated with a single exposure dose of 15 Gy of gamma rays to the head and neck region; irradiated (III), in which animals were only irradiated with a single exposure dose of 15 Gy of gamma rays; vitamin E (IV), in which animals received alpha tocopherol acetate solution but were not irradiated; irradiated-vitamin E (V), in which animals received alpha tocopherol acetate solution before irradiation with a single exposure dose of 15 Gy gamma rays. The animals were sacrificed 4, 8 h and 30 days after the irradiation procedure. No differences were observed in salivary volumes between the groups at 4 and 8 h. At 30 days, the salivary volume in the animals pertaining to the irradiated-olive oil group was significantly reduced in relation to the control group. The only irradiated group (III) presented significantly diminished salivary volume. In the salivary composition, no significant differences were observed in the total protein content between the groups studied. It was concluded that radiation had no effect on the total protein content and that vitamin E protected the salivary function 30 days after irradiation. Thus, vitamin E can be considered as a potential radioprotective substance.
Ramos
Flávia Maria de Moraes
FM
Oral Radiology, School of Dentistry of Piracicaba, University of Campinas, Piracicaba, São Paulo, SP, Brazil. flaviamaria@fop.unicamp.br
Pontual
Maria Luiza Dos Anjos
ML
de Almeida
Solange Maria
SM
Bóscolo
Frab Norberto
FN
Tabchoury
Cinthia Pereira Machado
CP
Novaes
Pedro Duarte
PD
D000818:Animals; D005720:Gamma Rays; D008297:Male; D010862:Pilocarpine; D011832:Radiation Injuries / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012469:Salivary Glands / Q000378:metabolism / Q000528:radiation effects*; D012471:Salivary Proteins and Peptides / Q000378:metabolism; D012472:Salivation / Q000528:radiation effects; D014810:Vitamin E / Q000627:therapeutic use*
D011837:Radiation-Protective Agents; D012471:Salivary Proteins and Peptides; D010862:Pilocarpine; D014810:Vitamin E