title: [Inhibitory effect of emodin on proliferation of human lung adenocarcinoma cells in vitro].
abstract: OBJECTIVE
  To investigate the anti-tumor mechanisms of emodin on human lung adenocarcinoma cell line Anip973 (LACC).
  
  
  METHODS
  The influence of emodin of different concentration at different time on LACC proliferation were determined and compared using MTT colorimeric assay and cell growth curve assay. And the cell apoptotic rate was determined by flow cytometry and analyzed with electronic microscope.
  
  
  RESULTS
  In a certain range, the higher concentration and longer acting time of emodin could induce the stronger inhibitory effect on tumor cell growth and the higher apoptotic rate. The proliferation inhibitory rate reached 90% after LACC being treated with emodin 60 micromol/L for 72h.
  
  
  CONCLUSIONS
  Emodin can significantly inhibit proliferation of human LACC, showing dose-effect and time-effect relationship.
authors.affiliation: Department of Respiratory Internal Diseases, The Second Hospital Affiliated to Harbin Medical University, Harbin. ljn1209475@sina.com.cn
authors.forename: Jia-ning
authors.initials: JN
authors.lastname: Li
authors.forename: Fu-zhen
authors.initials: FZ
authors.lastname: Lu
authors.forename: Jin-ling
authors.initials: JL
authors.lastname: Xiao
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Bao
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004642:Emodin / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004642:Emodin