title: Evaluation of histological techniques for the detection of fungal infections caused by Leptolegnia chapmanii (Oomycetes: Saprolegniales) in Aedes aegypti (Diptera: Culicidae) larvae.
abstract: We evaluated which of the fixatives and stains most frequently used for observation of insect tissues were the most appropriate for histopathological visualization of entomopathogenic fungal infections with Leptolegnia chapmanii in larvae of Aedes aegypti. The best contrast between the host tissues and the fungal structures was obtained when using a combination of Camoy fixative with Grocott staining contrasted with light green. Masson trichromic stain combined with 10% formaldehyde-phosphate buffer also provided satisfactory results--a good contrast and clearly distinguishable host tissues and fungal structures.
authors.affiliation: Centro de Estudios Parasitológicos y de Vectores, Consejo Nacional de Investigaciones Cientificas y Tecnicas de la República Argentina-Universidad Nacional de la Plata, La Plata, Argentina.
authors.forename: V E
authors.initials: VE
authors.lastname: Dikgolz
authors.forename: A V
authors.initials: AV
authors.lastname: Toledo
authors.forename: P E
authors.initials: PE
authors.lastname: Topa
authors.forename: C C
authors.initials: CC
authors.lastname: López Lastra
mesh_terms: D019342:Acetic Acid; D000330:Aedes / Q000254:growth & development / Q000382:microbiology*; D000818:Animals; D002725:Chloroform; D000431:Ethanol; D005404:Fixatives; D016591:Histocytological Preparation Techniques / Q000379:methods*; D007814:Larva / Q000382:microbiology; D009868:Oomycetes / Q000472:pathogenicity / Q000502:physiology / Q000648:ultrastructure*; D013172:Spores, Fungal / Q000648:ultrastructure
chemical_list: C033474:Carnoy's solution; D005404:Fixatives; D000431:Ethanol; D002725:Chloroform; D019342:Acetic Acid