Proteomic analysis of the outer membrane of Anabaena sp. strain PCC 7120.
Anabaena is a model to analyze the evolutionary development of plastids, cell differentiation, and the regulation of nitrogen fixation. Thereby, the outer membrane proteome is the place of sensing environmental differences and during plastid development, systems for intracellular communication had to be added to the proteome of this membrane. We present a protocol for the isolation of the outer membrane from Anabaena and the analysis of the proteome using different tools. 55 proteins were identified.
Moslavac
Suncana
S
Department of Biology I, LMU Munich, Menzinger Strasse 67, D-80638 Münich, Germany.
Bredemeier
Rolf
R
Mirus
Oliver
O
Granvogl
Bernhard
B
Eichacker
Lutz A
LA
Schleiff
Enrico
E
D017033:Anabaena / Q000166:cytology*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000302:isolation & purification*; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry*; D019295:Computational Biology; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*
D001425:Bacterial Outer Membrane Proteins; D020543:Proteome