title: Changes in reducing power profile of gastric juice in patients with active duodenal ulcer.
abstract: Reactive oxygen species have been postulated to play a role in the pathogenesis of mucosal GI injury and in peptic ulcer disease (PUD). The low molecular weight antioxidants (LMWA) group plays an important role in the defense mechanism of the GI tract against oxidative damage, and is a major component of the reducing capacity of biological tissues and fluids. We hypothesized that altered gastric LMWA anti oxidative status might play a role in the pathogenesis of upper GI disorders such as PUD and could be evaluated by measuring gastric juice reducing power. The aim of the present study was to determine, by cyclic voltammetry, changes in the overall antioxidant activity of the gastric juice in active duodenal ulcer (DU) obtained during upper endoscopy from patients as compared with normal subjects. The results show that in 28/37 (76%) of the control subjects, gastric juice demonstrated a reducing power of at least two anodic waves indicating at least two different LMWA groups. Three or more anodic waves were recorded in 12 normal subject (32%). In contrast, 16/25 (64%) of gastric juice samples obtained from active DU patients exhibited only one anodic wave usually at a high potential (>900 mV). These results imply that gastric juice normally possesses a reducing power profile that can be determined by cyclic voltammetry. This profile is significantly changed in untreated DU disease. These changes in active DU may indicate decreased gastric antioxidant activity reflecting reduced mucosal protection that leading to increased susceptibility of the gastro-duodenum to injury.
authors.affiliation: Gastroenterology Unit, Division of Medicine, Hebrew University Hadassah Medical Center, Ein Kerem. P.O. Box 12000, Jerusalem 91120, Israel. ligum@md.huji.ac.il
authors.forename: Moshe
authors.initials: M
authors.lastname: Ligumsky
authors.forename: Aharon
authors.initials: A
authors.lastname: Klar
authors.forename: Jimmy
authors.initials: J
authors.lastname: Siguencia
authors.forename: Ron
authors.initials: R
authors.lastname: Arnon
authors.forename: Irith
authors.initials: I
authors.lastname: Gati
authors.forename: Ron
authors.initials: R
authors.lastname: Kohen
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000032:analysis*; D004381:Duodenal Ulcer / Q000378:metabolism*; D005260:Female; D005745:Gastric Acidity Determination; D005750:Gastric Juice / Q000378:metabolism*; D016480:Helicobacter pylori / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D014510:Urease / Q000032:analysis
chemical_list: D000975:Antioxidants; D014510:Urease