[Purification and characterization of the proliferation of rat osteoblast-like cells UMR-106 from pilose antler].
OBJECTIVE
The activity of deer serum albumin on proliferation of rat osteogenic-like cells UMR-106 and the IGF-I secretion were investigated in order to elucidate pilose antler's bone-strengthening mechanism.


METHODS
Deer serum albumin was isolated from freeze-dry pilose antler powder extract. The methods were Sephacryl S-200HR gel filtration, POROS 20QE ion-exchange and TSK G3000SW chromatographies. The effect of deer serum albumin on proliferatio of UMR-106 cells was assaied by MTT, and the secretion of IGF-I of UMR-106 cells was assaied by RIA.


RESULTS
Deer serum albumin, with the molecular weight of 56.3 kDa, significantly increased the proliferation rate of the osteoblast-like UMR-106 cell and IGF-I secretion. When concentration of deer serum albumin reached 0.149 microg x mL(-1), UMR-106 cell proliferation rate was 241.03% and IGF-I secretion was 66.89 ng x mL(-1).


CONCLUSIONS
The concentration of deer serum albumin, from 14.9 ng x mL(-1) to 14.90 microg x mL(-1), significantly increased the proliferation rate of the osteoblast-like UMR-106 cell and IGF- I secretion.
Lin
Dong-Yun
DY
Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China.
Huang
Xiao-Nan
XN
Ke
Li-Jing
LJ
Chen
Xiao-Chao
XC
Ye
Xiu-Yun
XY
Huo
Yu-Shu
YS
Rao
Ping-Fan
PF
D000818:Animals; D000999:Antlers / Q000737:chemistry; D001859:Bone Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003670:Deer; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology; D012516:Osteosarcoma / Q000473:pathology*; D051381:Rats; D012709:Serum Albumin / Q000302:isolation & purification / Q000494:pharmacology*
D008419:Materia Medica; D012709:Serum Albumin; D007334:Insulin-Like Growth Factor I