Semisynthetic tellurosubtilisin with glutathione peroxidase activity.
Reaction of the hydroxyl group of serine-221 of subtilisin with phenylmethanesulfonylfluoride followed by nucleophilic substitution with sodium hydrogen telluride, a semisynthetic telluroprotein, tellurosubtilisin, was prepared. Tellurosubtilisin, which displays high substrate specificity for aromatic thiols, exhibits remarkable peroxidase activity and catalyzes the reduction of hydrogen peroxide by 3-carboxy-4-nitrobenzenethiol 20 000 times more efficiently than diphenyl diselenide.
Mao
Shizhong
S
Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130012, People's Republic of China.
Dong
Zeyuan
Z
Liu
Junqiu
J
Li
Xiangqiu
X
Liu
Xiaoman
X
Luo
Guimin
G
Shen
Jiacong
J
D002384:Catalysis; D005979:Glutathione Peroxidase / Q000737:chemistry*; D020860:Subtilisin / Q000138:chemical synthesis* / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry
D005979:Glutathione Peroxidase; D020860:Subtilisin; D013691:Tellurium