title: Glucose intolerance modifies the inflammatory response after intestinal ischemia-reperfusion.
abstract: Streptozotocin administration in newborn rats (nSTZ-rats) leads to adults with mild insulin deficiency and normoglycemia, and is accepted as a model of type 2 diabetes. We examined possible differences in the production of inflammatory mediators between healthy and nSTZ-rats after ischemia-reperfusion (I-R). Two-month-old control and nSTZ-rats were randomly separated into control and intestinal I-R groups. After reperfusion, samples were obtained from the portal vein (PV) infrahepatic cava vein (ICV), suprahepatic cava vein (SCV), jejunal wall, and pancreas. Nitric oxide (NO), lipid hydroperoxides (LPO), tumor necrosis factor alpha (TNF-alpha), 60 kDa receptor (sTNF-R1), 80 kDa (sTNF-R2), and intercellular adhesion molecule-1 (ICAM-1), were determined. After I-R, nSTZ-rats showed increased plasma concentrations of LPO, NO, ICAM-1 (0.5141 +/- 0.083 vs 0.024 +/- 0.003, ICV; 0.574 +/- 0.075 vs 0.023 +/- 0.003, SCV; 0.528 +/- 0.067 vs 0.027 +/- 0.003 PV; ng/ml), TNF-alpha (42.4 +/- 5.7 ICV, 248.4 +/- 28.2 SCV, and 33.6 +/- 4.0 PV. In n STZ-rats, vs 4.36 +/- 0.57, 4.74 +/- 0.77, and 3.16 +/- 0.32, respectively, in control rats; pg/ml), and sTNF-R1. Both TNF-alpha and NO plasma levels were higher in SCV than in ICV and PV after I-R. In addition, after I-R, jejunal wall of nSTZ-rats showed an increase of TNF-alpha IL-1, and IL-10 levels. A pre-existing state of glucose intolerance intensifies the inflammatory response after intestinal I-R.
authors.affiliation: School of Medicine and Surgery, Hospital San Carlos, Universidad Complutense, Madrid, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Garcia-Perez
authors.forename: Javier
authors.initials: J
authors.lastname: Arias-Diaz
authors.forename: Elena
authors.initials: E
authors.lastname: Vara
authors.forename: Jose L
authors.initials: JL
authors.lastname: Balibrea
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D018149:Glucose Intolerance / Q000097:blood* / Q000503:physiopathology; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D007422:Intestines / Q000098:blood supply*; D015227:Lipid Peroxidation; D008297:Male; D009569:Nitric Oxide / Q000097:blood; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018124:Receptors, Tumor Necrosis Factor / Q000097:blood; D015427:Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology; D018709:Statistics, Nonparametric; D013311:Streptozocin
chemical_list: D016207:Cytokines; D018124:Receptors, Tumor Necrosis Factor; D018799:Intercellular Adhesion Molecule-1; D009569:Nitric Oxide; D013311:Streptozocin