[Quantitative analysis of Danshensu in Tongmai Oral Solution by HPLC].
OBJECTIVE
To establish a method by RP-HPLC for determination of content of Danshensu (3,4-hydroxybenzyl lacticacid) in Tongmai Oral Solution.


METHODS
The samples were separated on an ODS column Hypersil C18 (10 microm, 4.6 mm x 200 mm) with the mobile phase 0. 5% acetic acid-methanol-dimethyl formamide (99:0.5:0.5). The flow rate was 1.2 ml/min, and the detection wavelength was set at 335nm.


RESULTS
The average recovery of Danshensu was 98.31%, and RSD was 2. 11%.


CONCLUSIONS
The method is efficient and accurate with a good repeatability and can be used for the quality control of Tongmai Oral Solution.
Huang
Chao
C
Shenzhen Traditional Chinese Medicine General Factory, Shenzhen.
Fang
Si
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D007773:Lactates / Q000032:analysis* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid