alpha,omega-Dicarboxylic acid accumulation by acyl-CoA oxidase deficient mutants of Yarrowia lipolytica.
alpha,omega-Dicarboxylic acid accumulation from alkanes and alkane degradation intermediates was investigated using Yarrowia lipolytica wild type strain W29 as well as a double, a triple and a quadruple POX-deleted strains. Six genes, POX1 through POX6, encode six acyl-CoA oxidase isozymes in Y. lipolytica. All the strains accumulated dodecanedioic acid (5-20 mg ml(-1)) from the diterminal functionalised 1,12-dodecane diol and 12-hydroxdodecanoic acid. The quadruple-deleted strain was the only strain that was able to accumulate dioic acids from C16 alkanol and monocarboxylic acid as well as from C12, C14 and C16 alkanes (maximum 8 mg ml(-1) from dodecane).
Smit
Martha S
MS
Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa. smitms.sci@mail.uovs.ac.za
Mokgoro
Masego M
MM
Setati
Evodia
E
Nicaud
Jean-Marc
JM
D042962:Acyl-CoA Oxidase / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D000473:Alkanes / Q000378:metabolism; D001711:Biotransformation; D003998:Dicarboxylic Acids / Q000378:metabolism*; D017353:Gene Deletion; D005838:Genotype; D007527:Isoenzymes / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D013997:Time Factors; D025062:Yarrowia / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
D000473:Alkanes; D003998:Dicarboxylic Acids; D007527:Isoenzymes; C036836:dodecanedioic acid; D042962:Acyl-CoA Oxidase