Protective effect of Eimeria stiedae coproantigen against hepatic coccidiosis in rabbits.
Immunization of rabbits against hepatic coccidiosis was tried. The animals were immunized twice with Eimeria stiedae coproantigen in freund's adjuvant with two week intervals. The rabbits were challenged orally with sporulated E. stiedae oocysts one week post last injection. The protection was assessed by number of oocysts output, number of focal liver lesions, clinical sings and antibody response. The immunization resulted in 70% protection from infection and decline in oocysts count. High level of IgG response in immunized rabbits than control infected ones was occurred and being responsible for the recorded protection. The electrophoretic make up of the coproantigen and oocyst antigen showed different patterns of separation. Common as well as specific bands to each antigen were identified. Using the rabbit sera after 3 weeks post vaccination in immunoblot assay, immunogenic components were detected of molecular weight 155, 103, 74, 66, 44, 22KD with coproantigen and 155, 115, 57, 26 KD with oocyst antigen. While, the rabbit sera after 2 and 4 weeks post challenge reacted with oocyts antigen, in immunoblot assay, revealing immunogenic bands of molecular weight 155, 115, 57, 26, 22KD and 155, 115, 57, 25 KD respectively. Bands of 22KD and 155KD are partially responsible for eliciting host protective immune response where they were recognized by immunized sera in coproantigen and by immunized infected sera in oocyst antigen.
Abdel Megeed
Kadria N
KN
Department of Parasitology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
Abuel Ezz
Nadia M T
NM
Abdel-Rahman
Eman H
EH
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology*; D003048:Coccidiosis / Q000517:prevention & control / Q000662:veterinary*; D004539:Eimeria / Q000276:immunology*; D005243:Feces / Q000469:parasitology; D007114:Immunization; D008099:Liver / Q000469:parasitology; D008109:Liver Diseases, Parasitic / Q000517:prevention & control / Q000662:veterinary*; D008297:Male; D011817:Rabbits / Q000276:immunology / Q000469:parasitology*; D011897:Random Allocation
D000909:Antibodies, Helminth; D000953:Antigens, Protozoan