title: [Specific and nonspecific interactions of antibodies and immunoglobulins with antigens and the methods of analysis of these interactions].
abstract: The problem of specific and nonspecific interaction of serum immunoglobulins and antigens was considered. It was shown that high-sensitive methods allow to reveal low-affinity non-specific interaction of immunoglobulins and antigens. If the concentration of the specific antibodies in a studied sample of serum is low, the non-specific interaction of serum immunoglobulins may exceed substantially the effect of specific reaction. In this case the obtained results could be misinterpreted. In this connection the conclusion has been done that in such a case it is necessary to take into account the capability of serum immunoglobulins to interact non-specifically with antigens and to discriminate between specific and non-specific interaction. The methods of the diminishing the non-specific interaction are suggested.
authors.forename: S A
authors.initials: SA
authors.lastname: Bobrovnik
mesh_terms: D000818:Animals; D000915:Antibody Affinity / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology*; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D001666:Binding Sites, Antibody; D002417:Cattle; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D009211:Myoglobin / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D009211:Myoglobin; D012710:Serum Albumin, Bovine; D010047:Ovalbumin