Protein expression profiling identifies maspin and stathmin as potential biomarkers of adenoid cystic carcinoma of the salivary glands.
Adenoid cystic carcinoma (ACC) is one of the most common malignant tumors of the salivary glands. It tends to grow slowly but is associated with a poor prognosis compared to other malignant salivary gland tumors. To identify specific markers of ACC, we examined protein expression profiling in ACC xenograft and normal salivary glands (NSG) using fluorescent 2-dimensional differential in-gel electrophoresis (2-D-DIGE), an emerging technique for comparative proteomics, that improves the reproducibility and reliability of differential protein expression analysis between the samples. To identify the proteins, matrix-assisted laser desorption/ionization time-of-flight peptide mass fingerprinting was carried out. Using these strategies, we detected 4 upregulated proteins and 5 downregulated proteins in ACC xenograft. Maspin and stathmin were selected for further analyses. Western blotting and immunohistochemical staining showed a higher expression of these proteins in ACC xenograft and clinical ACC tissue compared to NSG. Furthermore, Expression of these proteins was correlated with the histologic grading of ACC (n = 10). Therefore, our data indicate that maspin and stathmin may be not only useful biomarkers of ACC but also markers of biologic behavior in this tumor.
Nakashima
Dai
D
Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, Chiba, Japan.
Uzawa
Katsuhiro
K
Kasamatsu
Atsushi
A
Koike
Hirofumi
H
Endo
Yosuke
Y
Saito
Kengo
K
Hashitani
Susumu
S
Numata
Tsutomu
T
Urade
Masahiro
M
Tanzawa
Hideki
H
D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000556:secondary; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D040901:Proteomics; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D012469:Salivary Glands / Q000378:metabolism*; D015843:Serpins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D050777:Stathmin / Q000378:metabolism*; D014183:Transplantation, Heterologous
D014408:Biomarkers, Tumor; C085344:SERPIN-B5; D015843:Serpins; D050777:Stathmin