title: Macrophages direct tumour histology and clinical outcome in a colon cancer model.
abstract: Macrophages generally constitute a major component of the tumour stroma. Although conventionally considered to be cytotoxic effector cells, macrophages have recently been described as promoters of tumour progression. The present study shows that selective depletion of peritoneal or liver macrophages prior to CC531 tumour cell inoculation resulted in highly differentiated tumours in rats. In contrast, tumours from control rats, in which macrophages are abundantly present, showed a desmoplastic stromal reaction with hallmark features of malignancy, such as neovascularization and matrix remodelling, indicating that the presence of macrophages is associated with malignant histopathological differentiation. Remarkably, macrophage-depleted rats, bearing highly differentiated tumours, had a worse prognosis, as they displayed a higher tumour load and poorer survival. Thus, while macrophages direct tumours towards malignant tumour histology, their role in anti-tumour defence is important. The selective inhibition of macrophage functions involved in malignant progression without interfering with cytotoxic ability may therefore identify important new targets for cancer therapy.
authors.affiliation: Department of Surgical Oncology, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Oosterling
authors.forename: Gerben J
authors.initials: GJ
authors.lastname: van der Bij
authors.forename: Gerrit A
authors.initials: GA
authors.lastname: Meijer
authors.forename: Cornelis W
authors.initials: CW
authors.lastname: Tuk
authors.forename: Evert
authors.initials: E
authors.lastname: van Garderen
authors.forename: Nico
authors.initials: N
authors.lastname: van Rooijen
authors.forename: Sybren
authors.initials: S
authors.lastname: Meijer
authors.forename: Joost R M
authors.initials: JR
authors.lastname: van der Sijp
authors.forename: Robert H J
authors.initials: RH
authors.lastname: Beelen
authors.forename: Marjolein
authors.initials: M
authors.lastname: van Egmond
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D004195:Disease Models, Animal; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D007150:Immunohistochemistry / Q000379:methods; D007700:Kinetics; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary; D008264:Macrophages / Q000473:pathology*; D017737:Macrophages, Peritoneal / Q000473:pathology; D008297:Male; D009389:Neovascularization, Pathologic / Q000473:pathology; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D046888:Tissue Array Analysis / Q000379:methods
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm