title: In vitro synthesis of enzymes of the tryptophan operon of Escherichia coli.
abstract: In vitro synthesis of enzymes of the tryptophan (trp) operon of E. coli was studied in an extract prepared from E. coli, which is programmed with purified DNA from trp transducing phages with mutations that effect the expression of the trp genes in various ways. Our results show that control of transcription, i.e. initiation and termination, is similar in vivo and in vitro.
authors.affiliation: Medical Biological Laboratory TNO, Rijswijk Z. H., The Netherlands.
authors.forename: P H
authors.initials: PH
authors.lastname: Pouwels
authors.forename: J
authors.initials: J
authors.lastname: van Rotterdam
mesh_terms: D000878:Anthranilate Synthase / Q000096:biosynthesis* / Q000235:genetics; D003090:Coliphages / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology*; D009154:Mutation; D009876:Operon; D011401:Promoter Regions, Genetic; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D014364:Tryptophan / Q000235:genetics*; D014367:Tryptophan Synthase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014364:Tryptophan; D000878:Anthranilate Synthase; D014367:Tryptophan Synthase