title: Role of the 5'-UTR in accumulation of the rbpA1 transcript at low temperature in the cyanobacterium Anabaena variabilis M3.
abstract: The expression of the rbp genes, which encode RNA-binding proteins with a single RNA-recognition motif and a glycine-rich sequence, is known to increase at low temperature in cyanobacteria. We previously showed that their regulation involved both transcription and mRNA stability. In the present study, various reporter constructs with deletions and mutations were used to analyze this regulation, revealing that at least the following three elements are involved. First, a putative enhancer element is located within the upstream gene. Second, the rbpA1 transcript is dramatically stabilized by a large stem-loop structure located at the 5' terminus. Third, the transcript is also destabilized by a downstream box located within the coding region.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, 255 Shimo-Ohkubo, Sakura, Saitama 338-8570, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Ehira
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ohmori
authors.forename: Naoki
authors.initials: N
authors.lastname: Sato
mesh_terms: D020121:5' Untranslated Regions / Q000502:physiology*; D046870:Anabaena variabilis / Q000235:genetics* / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics; D003080:Cold Temperature; D004742:Enhancer Elements, Genetic; D015964:Gene Expression Regulation, Bacterial; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics*; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: D020121:5' Untranslated Regions; D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D016601:RNA-Binding Proteins