title: [Subcellular localization and tissues expression profile of hGPCRc: an orphan G protein-coupled receptor].
abstract: As a member of orphan G protein-coupled receptors (oGPCRs), hGPCRc was cloned from human colon tissue and analyzed by bioinformatic softwares. It was showed that the corresponding amino acids of hGPCRc formed seven-transmembrane domains as the key characteristic of GPCRs. Then, the recombinant GFP-hGPCRc was constructed by fussing hGPCRc into pEGFP-N1 carrying green fluorescent protein (GFP) gene, and CHO-K1 cells were subsequently transfected with the GFP-hGPCRc or pEGFP-N1. The green fluorescence protein expression in the two different transfected cells was observed under the laser scanning confocal microscopy (LSCM). It was showed that green fluorescence protein was distributed in the whole bodies of the cells transfected with pEGFP-N1, but mainly distributed on the plasma membrane and cytoplasm membrane transfected with GFP-hGPCRc. Thus, the localization on the membrane of hGPCRc was accorded with the predication by bioinformatic analysis. The expression analysis of hGPCRc by RT-PCR indicated that hGPCRc was abundantly expressed in heart, kidney, cerebel and colon etc., but absent in liver, cerebra, small intestine and muscle etc. The expressing profile of hGPCRc could provide some useful clues to understanding its effects on embryonic development and physiological functions.
authors.affiliation: Institute of Maize, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Guang-Sheng
authors.initials: GS
authors.lastname: Yuan
authors.forename: Guang-Tang
authors.initials: GT
authors.lastname: Pan
authors.forename: Fang-Ming
authors.initials: FM
authors.lastname: Wu
authors.forename: Chun-Guang
authors.initials: CG
authors.lastname: Han
authors.forename: Huo-Gao
authors.initials: HG
authors.lastname: Huang
authors.forename: Ming
authors.initials: M
authors.lastname: Hu
authors.forename: Li
authors.initials: L
authors.lastname: Sheng
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Yong-Xue
authors.initials: YX
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution; D014162:Transfection
chemical_list: C531500:GPR22 protein, human; D043562:Receptors, G-Protein-Coupled; D049452:Green Fluorescent Proteins