[Effect of l-tetrahydropalmatine on expression of adhesion molecules induced by lipopolysaccharides in human umbilical vein endothelium cell].
OBJECTIVE
To investigate the effect of l-tetrahydropalmatine (l-THP) on adhesion molecule expression induced by LPS in human umbilical vein endothelium cell (HUVEC).


METHODS
HUVEC were obtained from the human umbilical cord vein and treated by LPS or LPS plus different concentration of l-THP. Expression of ICAM-1 and E-selectin was assayed by flow cytometer.


RESULTS
l-THP(250 mg x L(-1)) markedly attenuated ICAM-1 and E-selectin expression induced by LPS(P <0.05). l-THP(50 mg x L(-1) inhibited E-selectin expression (P < 0.05) but had no effect on ICAM-1 (P > 0.05). l-THP (10 mg x L(-1)) had no effect on expression of both adhesion molecules. DXM (50 mg x L(-1)) completely inhibited both responses induced by LPS (P < 0.05).


CONCLUSIONS
l-THP depresses expression of ICAM-1 and E-selectin induced by LPS, which suggests that I-THP represent a promising candidate to be developed into therapies for the treatment of inflammation.
Zhang
Zhong-Miao
ZM
2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China. zzm681126@hotmail.com
Jiang
Bo
B
Zheng
Xiao-Xiang
XX
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D001600:Berberine Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D019040:E-Selectin / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors; D014471:Umbilical Veins / Q000166:cytology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001600:Berberine Alkaloids; D019040:E-Selectin; D008070:Lipopolysaccharides; D018799:Intercellular Adhesion Molecule-1; C014215:tetrahydropalmatine