Ran-GTP regulates kinetochore attachment in somatic cells.
The Ran GTPase controls multiple mitotic processes in Xenopus egg extracts, including mitotic checkpoints, spindle assembly and post-mitotic nuclear envelope reassembly. We have analyzed Ran's role in somatic cells. We uncovered a novel mitotic role of Ran-GTP, involving the Crm1 nuclear export receptor. This pathway is an important mode of Ran-GTP function during mitosis in mammalian somatic cells, which mediates the recruitment of the RanGAP1/RanBP2 complex to kinetochores and maintains the microtubule-based fibers connecting kinetochores to spindle poles (k-fibers). Here we discuss potential implications of these findings for normal k-fiber assembly.
Arnaoutov
Alexei
A
Laboratory of Gene Regulation and Development, NICHD, NIH, Bethesda, Maryland 20892-5431, USA.
Dasso
Mary
M
D021581:Active Transport, Cell Nucleus; D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000378:metabolism; D016678:Genome; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000737:chemistry; D028884:Karyopherins / Q000378:metabolism; D018386:Kinetochores / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D008954:Models, Biological; D018832:Molecular Chaperones / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism; D014981:Xenopus; D029867:Xenopus Proteins / Q000378:metabolism; D020931:ran GTP-Binding Protein / Q000502:physiology*; D000097606:Exportin 1 Protein
D018797:Cell Cycle Proteins; D020690:GTPase-Activating Proteins; D020662:Guanine Nucleotide Exchange Factors; D006160:Guanosine Triphosphate; D028884:Karyopherins; D018832:Molecular Chaperones; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D029867:Xenopus Proteins; D020931:ran GTP-Binding Protein; D000097606:Exportin 1 Protein; C499304:RCC1 protein, Xenopus; C513268:RanGAP1 protein, Xenopus; C094493:ran-binding protein 2