title: Influenza A H5N1 detection.
abstract: We developed a sensitive and rapid real-time reverse transcription-polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinical samples. This assay was evaluated with samples from H5N1-infected patients and demonstrated greater sensitivity and faster turnaround time than nested RT-PCR.
authors.affiliation: Virology Division, Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region, China.
authors.forename: Enders K O
authors.initials: EK
authors.lastname: Ng
authors.forename: Peter K C
authors.initials: PK
authors.lastname: Cheng
authors.forename: Antia Y Y
authors.initials: AY
authors.lastname: Ng
authors.forename: T L
authors.initials: TL
authors.lastname: Hoang
authors.forename: Wilina W L
authors.initials: WW
authors.lastname: Lim
mesh_terms: D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics; D006388:Hemagglutinins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000956:Antigens, Viral; D006388:Hemagglutinins; D012367:RNA, Viral