Release of IL-1beta via IL-1beta-converting enzyme in a skin dendritic cell line exposed to 2,4-dinitrofluorobenzene.
We used a mouse fetal skin dendritic cell line (FSDC) to study the effect of the strong allergen 2,4-dinitrofluorobenzene (DNFB) on interleukin (IL)-1beta release and IL-1beta receptor immunoreactivity. Stimulation with DNFB (30 minutes) increased IL-1 release without changing the mRNA levels of the protein. Furthermore, DNFB increased transiently the interleukin-1beta-converting enzyme (ICE) activity, as measured with its fluorogenic substrate Z-Tyr-Val-Ala-Asp-AFC. The ICE inhibitor Z-YVAD-FMK prevented the release of IL-1beta evoked by DNFB. Incubation of the cells with DNFB (30 minutes) strongly increased IL-1beta receptor immunoreactivity. The rapid effect of DNFB on the release of mature IL-1beta, without inducing an increase of IL-1beta mRNA in FSDC, suggests a posttranslational modification of pro-IL-1beta by ICE activity.
Matos
Teresa J
TJ
Centro de Neurosciências e Biologia Celular, Universidade de Coimbra, Portugal.
Jaleco
Sara P
SP
Gonçalo
Margarida
M
Duarte
Carlos B
CB
Lopes
M Celeste
MC
D000590:Amino Acid Chloromethyl Ketones / Q000378:metabolism; D000818:Animals; D020170:Caspase 1 / Q000378:metabolism*; D061945:Caspase Inhibitors; D002460:Cell Line; D015853:Cysteine Proteinase Inhibitors / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D004139:Dinitrofluorobenzene / Q000276:immunology / Q000494:pharmacology*; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011499:Protein Processing, Post-Translational; D017472:Receptors, Interleukin-1 / Q000378:metabolism; D012867:Skin / Q000166:cytology*
D000590:Amino Acid Chloromethyl Ketones; D061945:Caspase Inhibitors; D015853:Cysteine Proteinase Inhibitors; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; C460579:benzyloxycarbonyltyrosyl-valyl-alanyl-aspartic acid fluoromethyl ketone; D004139:Dinitrofluorobenzene; D020170:Caspase 1