Expression of a novel apoptosis inhibitor-survivin in colorectal carcinoma.
OBJECTIVE
To investigate the role of survivin expression in the pathogenesis of colorectal carcinoma.


METHODS
Immunohistochemistry S-P method and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) were used to detect the expression of survivin and apoptotic cell in situ in colorectal cancerous tissues, para-cancerous tissues and normal tissues of 48 cases of colorectal carcinoma.


RESULTS
The survivin positive unit (PU) was higher in cancerous tissues (38.76+/-5.14) than in para-cancerous (25.17+/-7.26) or normal tissues (0.57+/-0.03) (P<0.05). The apoptosis index (AI) of para-cancerous tissues was (7.51+/-2.63%) higher than cancerous tissues (4.65+/-1.76%). The expression of survivin was associated with pathological grade, lymph node metastasis and Dukes stage of colorectal carcinoma.


CONCLUSIONS
Survivin expression may play an important role in carcinogenesis of colorectal carcinoma and may be associated with malignant biological behaviors of colorectal carcinoma.
Tan
Hai-Yan
HY
Department of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China. liu9861jun@yahoo.com.cn
Liu
Jun
J
Wu
Shan-Min
SM
Luo
He-Sheng
HS
D000328:Adult; D000368:Aged; D017209:Apoptosis; D015179:Colorectal Neoplasms / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D000077022:Survivin
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D000077022:Survivin