title: Mucosal and systemic immunization elicited by Newcastle disease virus (NDV) transgenic plants as antigens.
abstract: As a step towards developing a safe and effective edible vaccine against Newcastle disease virus (NDV), we have explored the use of plants genetically engineered to express viral proteins. We report the construction of transgenic potato plants expressing the genes coding for immunogenic proteins of NDV under the regulation of CaMV 35S promoter and its immunogenicity in mice. All mice receiving transgenic plant extracts in incomplete Freund adjuvant produced specific anti-NDV antibodies. Animals fed with transgenic leaves showed a specific response against NDV. Detection of IgA released from in vitro-cultured intestinal tissue fragments indicated the presence of IgA-secreting cells in the gut.
authors.affiliation: Instituto de Biotecnología, CICVyA, INTA, Cc25 B1712WAA, Castelar, Buenos Aires, Argentina. aberinst@cicv.inta.gov.ar
authors.forename: Analia
authors.initials: A
authors.lastname: Berinstein
authors.forename: Cecilia
authors.initials: C
authors.lastname: Vazquez-Rovere
authors.forename: Sebastian
authors.initials: S
authors.lastname: Asurmendi
authors.forename: Evangelina
authors.initials: E
authors.lastname: Gómez
authors.forename: Flavia
authors.initials: F
authors.lastname: Zanetti
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Zabal
authors.forename: Alejandro
authors.initials: A
authors.lastname: Tozzini
authors.forename: Daniela
authors.initials: D
authors.lastname: Conte Grand
authors.forename: Oscar
authors.initials: O
authors.lastname: Taboga
authors.forename: Gabriela
authors.initials: G
authors.lastname: Calamante
authors.forename: Hebe
authors.initials: H
authors.lastname: Barrios
authors.forename: Esteban
authors.initials: E
authors.lastname: Hopp
authors.forename: Elisa
authors.initials: E
authors.lastname: Carrillo
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000917:Antibody Formation; D018928:Immunity, Mucosal / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009521:Newcastle Disease / Q000276:immunology* / Q000517:prevention & control; D009522:Newcastle disease virus / Q000235:genetics* / Q000276:immunology; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines