title: Multivalent liposome-based vaccines containing different serosubtypes of PorA protein induce cross-protective bactericidal immune responses against Neisseria meningitidis.
abstract: Four serosubtypes (P1.7, 16, P1.7-2, 4, P1.19, 15 and P1.5-1, 10-4) of the PorA outer-membrane protein of Neisseria meningitidis were purified as recombinant proteins and incorporated into liposomes to investigate their immunogenicity. Each serosubtype induced high levels of bactericidal activity against the homologous strain. In addition, liposome preparations containing multiple serosubtypes induced high levels of bactericidal activity against each of the four strains. Significantly, antisera raised against monovalent and multivalent liposomes also showed cross-reactive bactericidal activity against heterologous strains. These data demonstrate that multivalent liposome vaccines, containing multiple PorA serosubtypes, have the potential to provide protection against a broad range of meningococcal strains.
authors.affiliation: Molecular Microbiology Group, Division of Infection, Inflammation and Repair, University of Southampton Medical School, Southampton General Hospital, Mailpoint 814, Level C, Lab and Path Block, Tremona Road, Southampton SO16 6YD, UK.
authors.forename: Holly E
authors.initials: HE
authors.lastname: Humphries
authors.forename: Jeannette N
authors.initials: JN
authors.lastname: Williams
authors.forename: Ramona
authors.initials: R
authors.lastname: Blackstone
authors.forename: Keith A
authors.initials: KA
authors.lastname: Jolley
authors.forename: Ho M
authors.initials: HM
authors.lastname: Yuen
authors.forename: Myron
authors.initials: M
authors.lastname: Christodoulides
authors.forename: John E
authors.initials: JE
authors.lastname: Heckels
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D003429:Cross Reactions; D007106:Immune Sera / Q000276:immunology; D008081:Liposomes; D022401:Meningococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009345:Neisseria meningitidis / Q000276:immunology; D018272:Porins / Q000235:genetics / Q000276:immunology*; D012703:Serotyping; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D007106:Immune Sera; D008081:Liposomes; D022401:Meningococcal Vaccines; D018272:Porins; D014614:Vaccines, Synthetic; C057699:porin protein, Neisseria