Preliminary x-ray crystallographic analysis of centrin from ciliate Euplotes octocarinatus.
Centrins are four-EF-hand Ca(2+)-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 angstroms. The crystals belong to space group P2(1)2(1)2(1) and the unit-cell parameters are a=34.442 angstroms, b=48.954 angstroms, c=72.583 angstroms.
He
Xiao-Jing
XJ
Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China.
Li
Liu
L
Hou
Hai
H
Zhao
Yaqin
Y
Liang
Aihua
A
Rao
Zihe
Z
D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D002923:Cilia; D018360:Crystallography, X-Ray; D020832:EF Hand Motifs; D016812:Euplotes / Q000737:chemistry*; D015800:Protozoan Proteins / Q000737:chemistry*
D002135:Calcium-Binding Proteins; D015800:Protozoan Proteins