[Extraction and purification of the Klebsiella pneumoniae capsular polysaccharide and the effection on the cell immunoactivity].
Klebsiella pneumoniae was cultured followed by the preparation and immunoactivity elucidating of its polysaccharide (CPS). The lysis of cell is the first key step in the preparation, under the co-action of trypsin, lysozyme and NP-40, the cell lysed within 2h, then the lysate was concentrated by ultrafiltration which serves as concentrating and partial purifying action simultaneously. Crude CPS was got by ethanol precipitation, then purified through the Ion-exchange and gel filtration, the purity of CPS was judged by the gel filtration and agarose gel electrophoresis. The effect of CPS on the cell immunoactivity was studied in detail, the results show that CPS possesses bidirectional immunoregulation on the spleen cells of mice, that is, low concentration of CPS can stimulate the immune response while the high concentration manifests the inhibition significantly. The investigation results will benefit on the exploitation of the CPS.
Zhang
Yan
Y
College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300222, China. yzhang@tust.edu.cn
Li
Qing-Zhou
QZ
Du
Lian-Xiang
LX
Qi
Wei
W
Chen
Jin-Ying
JY
D000818:Animals; D016667:Bacterial Capsules / Q000737:chemistry*; D003470:Culture Media; D007711:Klebsiella pneumoniae / Q000737:chemistry* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial / Q000276:immunology* / Q000302:isolation & purification*; D013154:Spleen / Q000166:cytology
D003470:Culture Media; D011135:Polysaccharides, Bacterial