Screening of cry2 genes in Bacillus thuringiensis isolates from Argentina.
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identification of cry2 genes from Bacillus thuringiensis (Bt) was established. Strains from different sources of Argentina were analyzed to study the distribution of cry2 genes. The results showed that cry2Aa/cry2Ab profile was the most abundant irrespective of source and represented 56 of 59 Bt isolates (94.9%). Three different cry2 profiles were found in this collection, one of which was novel.
Sauka
Diego H
DH
Area Bioinsumos Microbianos, Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria, De los Reseros y Las Cabañas, Castelar, Buenos Aires, Argentina. dsauka@cnia.inta.gov.ar
Cozzi
Jorge G
JG
Benintende
Graciela B
GB
D000818:Animals; D001118:Argentina; D001413:Bacillus thuringiensis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D004731:Endotoxins / Q000235:genetics* / Q000378:metabolism; D004783:Environmental Microbiology; D006460:Hemolysin Proteins; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis