Approaches to bacterial RNA isolation and purification for microarray analysis of Escherichia coli K1 interaction with human brain microvascular endothelial cells.
We established a protocol for isolation of microarray-grade bacterial RNA from Escherichia coli K1 interacting with human brain microvascular endothelial cells. The extracted RNA was free of human RNA contamination. More importantly, microarray analysis demonstrated that no bias was introduced in the gene expression pattern during the RNA isolation procedure.
Di Cello
Francescopaolo
F
Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Park 256, Baltimore, MD 21287, USA.
Xie
Yi
Y
Paul-Satyaseela
Maneesh
M
Kim
Kwang Sik
KS
D001921:Brain / Q000098:blood supply / Q000382:microbiology*; D042783:Endothelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D008833:Microcirculation / Q000382:microbiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012329:RNA, Bacterial / Q000302:isolation & purification*
D012329:RNA, Bacterial