Analysis of mitogen-activated protein kinase activation.
The biological functions of mast cells are regulated by several protein kinases, including the tyrosine kinases Fyn, Lyn, Syk, and FAK and the serine/threonine kinases Akt and PKC alpha/beta. The mitogen-activated protein kinases extracellular signal-regulated kinases, JNK, and p38MAPK also play a significant role in the regulation of mast cell biological function. This chapter will detail recent advances in determining mitogen-activated protein kinase activation in single cells. These methods are applicable to studies of signal transduction in mast cells.
Armstrong
Stephen C
SC
Cardiovascular Research Institute, University of South Dakota School of Medicine, Sioux Falls, SD, USA.
D000818:Animals; D045163:Antibodies, Phospho-Specific / Q000378:metabolism; D004789:Enzyme Activation; D007511:Ischemia; D020935:MAP Kinase Signaling System / Q000502:physiology; D008407:Mast Cells / Q000201:enzymology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010766:Phosphorylation; D011817:Rabbits
D045163:Antibodies, Phospho-Specific; D020928:Mitogen-Activated Protein Kinases