Species identification of mutans streptococci by groESL gene sequence.
The near full-length sequences of the groESL genes were determined and analysed among eight reference strains (serotypes a to h) representing five species of mutans group streptococci. The groES sequences from these reference strains revealed that there are two lengths (285 and 288 bp) in the five species. The intergenic spacer between groES and groEL appears to be a unique marker for species, with a variable size (ranging from 111 to 310 bp) and sequence. Phylogenetic analysis of groES and groEL separated the eight serotypes into two major clusters. Strains of serotypes b, c, e and f were highly related and had groES gene sequences of the same length, 288 bp, while strains of serotypes a, d, g and h were also closely related and their groES gene sequence lengths were 285 bp. The groESL sequences in clinical isolates of three serotypes of S. mutans were analysed for intraspecies polymorphism. The results showed that the groESL sequences could provide information for differentiation among species, but were unable to distinguish serotypes of the same species. Based on the determined sequences, a PCR assay was developed that could differentiate members of the mutans streptococci by amplicon size and provide an alternative way for distinguishing mutans streptococci from other viridans streptococci.
Hung
Wei-Chung
WC
Department of Clinical Laboratory Sciences and Medical Biotechnology1, Center for Optoelectronic Biomedicine2 and Department of Microbiology5, National Taiwan University College of Medicine, Taipei, Taiwan 3,4,6Division of Neurosurgery, Department of Surgery3, Department of Laboratory Medicine4 and Department of Dentistry6, National Taiwan University Hospital, Taipei, Taiwan.
Tsai
Jui-Chang
JC
Department of Clinical Laboratory Sciences and Medical Biotechnology1, Center for Optoelectronic Biomedicine2 and Department of Microbiology5, National Taiwan University College of Medicine, Taipei, Taiwan 3,4,6Division of Neurosurgery, Department of Surgery3, Department of Laboratory Medicine4 and Department of Dentistry6, National Taiwan University Hospital, Taipei, Taiwan.
Hsueh
Po-Ren
PR
Department of Clinical Laboratory Sciences and Medical Biotechnology1, Center for Optoelectronic Biomedicine2 and Department of Microbiology5, National Taiwan University College of Medicine, Taipei, Taiwan 3,4,6Division of Neurosurgery, Department of Surgery3, Department of Laboratory Medicine4 and Department of Dentistry6, National Taiwan University Hospital, Taipei, Taiwan.
Chia
Jean-San
JS
Department of Clinical Laboratory Sciences and Medical Biotechnology1, Center for Optoelectronic Biomedicine2 and Department of Microbiology5, National Taiwan University College of Medicine, Taipei, Taiwan 3,4,6Division of Neurosurgery, Department of Surgery3, Department of Laboratory Medicine4 and Department of Dentistry6, National Taiwan University Hospital, Taipei, Taiwan.
Teng
Lee-Jene
LJ
Department of Clinical Laboratory Sciences and Medical Biotechnology1, Center for Optoelectronic Biomedicine2 and Department of Microbiology5, National Taiwan University College of Medicine, Taipei, Taiwan 3,4,6Division of Neurosurgery, Department of Surgery3, Department of Laboratory Medicine4 and Department of Dentistry6, National Taiwan University Hospital, Taipei, Taiwan.
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015373:Bacterial Typing Techniques; D018835:Chaperonin 10 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018834:Chaperonin 60 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018833:Chaperonins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013045:Species Specificity; D013295:Streptococcus mutans / Q000145:classification* / Q000235:genetics
D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D004269:DNA, Bacterial; C097970:GroESL protein, Bacteria; D018833:Chaperonins