title: Time course of pelvic endometriotic lesion revascularization in a nude mouse model.
abstract: OBJECTIVE
  To assess the timing of endometrial lesion revascularization in a murine model.
  
  
  METHODS
  Prospective experimental study.
  
  
  METHODS
  An academic research environment.
  
  
  METHODS
  Twenty-six nude mice.
  
  
  METHODS
  Endometriosis was induced in mice by intraperitoneal deposition of human menstrual endometrium. Endometrial implants were recovered on days 1, 3, 5, 8, 10, 15, and 21 after implantation.
  
  
  METHODS
  Sections from the endometrial implants were immunostained with species-specific antiplatelet endothelial cell adhesion molecule-1 (PECAM-1) antibodies and vessels of murine and human origin were counted.
  
  
  RESULTS
  Endothelial cells of human origin in the implant progressively disappeared between day 3 and day 10. Seventy-eight percent of the vessel sections were positive for human PECAM-1 on day 5, 40.1% on day 8, and only 14.1% on day 10. However, there was a marked increase in murine PECAM-1-expressing vessels in endometrial stroma between day 5 (1.4%) and day 8 (68.0%), 10 (69.5%), and 15 (87.2%).
  
  
  CONCLUSIONS
  Our study demonstrates that PECAM-1 is a reliable endothelial cell marker to evaluate the role of angiogenesis in the nude mouse model. It also indicates that revascularization of human endometrial implants occurs between 5 and 8 days after implantation and involves the disappearance of native graft vessels, coinciding with the invasion of the interface and then the stroma by murine vessels.
authors.affiliation: Department of Gynaecology, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: Julie
authors.initials: J
authors.lastname: Eggermont
authors.forename: Jacques
authors.initials: J
authors.lastname: Donnez
authors.forename: Francoise
authors.initials: F
authors.lastname: Casanas-Roux
authors.forename: Hélène
authors.initials: H
authors.lastname: Scholtes
authors.forename: Anne
authors.initials: A
authors.lastname: Van Langendonckt
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology*; D004717:Endometrium / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology*; D010388:Pelvis / Q000098:blood supply / Q000473:pathology* / Q000502:physiology; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis; D013997:Time Factors
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1