Identification of four novel HLA-DOA alleles, DOA*010106, DOA*0102, DOA*0103, and DOA*0104N, by sequence-based typing*.
DOA sequences are currently known to have identical protein sequences. However, in this study, we report four novel allele types of human leucocyte antigen-DOA, including one synonymous and three non- synonymous amino acid changes from the Korean population. DOA*010106 has identical protein sequence with previously known DOA*010103 except one nucleotide difference at codon 45 (TCG-->TCA). In contrast, DOA*0102 and DOA*0103 have a sequence change at codon 99 (CTG-->GTG) and codon 105 (CGC-->TGC), causing non-synonymous amino acid changes, Leu99Val and Arg105Cys, respectively. In addition, DOA*0104N has a sequence deletion at codon 36 (CCC-->CC-), resulting in a frame shift leading to a stop codon at codon 62.
Moon
S-M
SM
National Genome Research Institute, National Institute of Health, Eunpyung-Gu, Seoul, Korea.
Gu
H
H
Ryu
H-J
HJ
Kim
J-J
JJ
Kim
H-T
HT
Han
B-G
BG
Kimm
K
K
Lee
J-K
JK
Oh
B
B
D000483:Alleles; D001483:Base Sequence; D003062:Codon; D018388:Codon, Terminator; D017931:DNA Primers / Q000235:genetics; D016368:Frameshift Mutation; D005802:Genes, MHC Class II; D006681:HLA-D Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D007723:Korea; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D018388:Codon, Terminator; D017931:DNA Primers; D006681:HLA-D Antigens