[Production of functional lipids by lipase-catalyzed acidolysis of lard in solvent free system].
China has richly and inexpensive fat and oils from animal and plants, but these resources could not get effectively utilization. In order to make the best of these resources, lipase-catalyzed acidolysis of lard with caprylic acid to produce functional lipid in solvent free system was investigated. Of the five lipases that were tested in the initial screening, immobilized lipase TL IM fromca T. languginosa resulted in the highest incorporation of capry lic acid into lard. This enzyme was further studied for the effect of enzyme load, substrate ratib, reaction time, reaction temperature and added water content on the incorporation of caprylic acid into lard. HPLC analyzed the products from the acidolysis reaction. The highest incorporation was attained at 20% enzyme load. Time course studied suggest that the incorporation of caprylic acid into lard was increased up to 38.77 mol% after 24h. Desirable mole ratio of substrates was 1:2 (lard: caprylic acid), caprylic acid incorporation up to 30.95 mol%. In the range of 45 - 60 degrees C , temperature had no significant effect on enzyme activity and caprylic acid incorporation changed little. When temperature was above 60 degrees C, incorporation of caprylic acid into lard was decreased. The highest incorporation of caprylic acid into lard 35.76 mol% was attained when added water content was 2.5%.
Zhao
Hai-Zhen
HZ
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Lu
Zhao-Xin
ZX
Bie
Xiao-Mei
XM
Lü
Feng-Xia
FX
Liu
Zhan-Min
ZM
D002210:Caprylates / Q000737:chemistry*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004041:Dietary Fats / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D008055:Lipids / Q000138:chemical synthesis*; D012997:Solvents
D002210:Caprylates; D004041:Dietary Fats; D004800:Enzymes, Immobilized; D008055:Lipids; D012997:Solvents; D008049:Lipase; C031492:octanoic acid; C029310:lard