title: Crohn's disease in Japanese is associated with a SNP-haplotype of N-acetyltransferase 2 gene.
abstract: OBJECTIVE
  To investigate the frequency and distribution of N-acetyltransferase 2 (NAT2) and uridine 5'-diphosphate (UDP)-glucuronosyltransferase 1A7 (UGT1A7) genes in patients with ulcerative colitis (UC) and Crohn's disease (CD).
  
  
  METHODS
  Frequencies and distributions of NAT2 and UGT1A7 SNPs as well as their haplotypes were investigated in 95 patients with UC, 60 patients with CD, and 200 gender-matched, unrelated, healthy, control volunteers by PCR-restriction fragment length polymorphism (RFLP), PCR-denaturing high-performance liquid chromatography (DHPLC), and direct DNA sequencing.
  
  
  RESULTS
  Multiple logistic regression analysis revealed that the frequency of haplotype, NAT2*7B, significantly increased in CD patients, compared to that in controls (P = 0.0130, OR = 2.802, 95%CI = 1.243-6.316). However, there was no association between NAT2 haplotypes and UC, or between any UGT1A7 haplotypes and inflammatory bowel disease (IBD).
  
  
  CONCLUSIONS
  It is likely that the NAT2 gene is one of the determinants for CD in Japanese. Alternatively, a new CD determinant may exist in the 8p22 region, where NAT2 is located.
authors.affiliation: Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Machida
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tsukamoto
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Wen
authors.forename: Saburou
authors.initials: S
authors.lastname: Shikuwa
authors.forename: Hajime
authors.initials: H
authors.lastname: Isomoto
authors.forename: Yohei
authors.initials: Y
authors.lastname: Mizuta
authors.forename: Fuminao
authors.initials: F
authors.lastname: Takeshima
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Murase
authors.forename: Naomichi
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Ikuo
authors.initials: I
authors.lastname: Murata
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
authors.forename: Chen-Yang
authors.initials: CY
authors.lastname: Wen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D003093:Colitis, Ulcerative / Q000201:enzymology*; D003424:Crohn Disease / Q000201:enzymology* / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012016:Reference Values
chemical_list: D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human