title: A statistical mechanical deconvolution of the differential scanning calorimetric profiles of the thermal denaturation of cyanomethemoglobin.
abstract: A differential scanning calorimetric study of the thermal unfolding of horse cyanomethemoglobin (as an irreversible protein system) was carried out in phosphate-EDTA buffer (20 mM phosphate, 1 mM EDTA) pH 7.2. The calorimetric rescanning of the protein solution was found to be irreversible and the process unfolded state --> final state appears to follow first order kinetic. Assuming the system to be comprised of n reversible states and one irreversible final state, the number of particles participating in the reversible states changes with time because they ultimately transit to the final irreversible denatured state. Hence, we carried out the deconvolution analysis using the grand canonical ensembles instead of just the canonical ensembles. This change was effected by introducing a correction term into the related equations which determines the outlet share of those particles exiting from the reversible states and converting into the final irreversible state. This approach provided an improved interpretation of the experimental data, which supports the following two-step process for the thermal denaturation of cyanomethemoglobin: alpha(2)beta(2) --> (alpha + alphabeta + beta)(excited) --> alpha(melt) + (alphabeta)(melt) + (beta(melt).
authors.affiliation: Department of Biology, Faculty of Science, University of Tehran, Tehran, Iran. safarian@khayam.ut.ac.ir
authors.forename: Shahrokh
authors.initials: S
authors.lastname: Safarian
authors.forename: Masoud
authors.initials: M
authors.lastname: Alimohammadi
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Saberi
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Moosavi-Movahedi
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D006736:Horses; D006358:Hot Temperature; D008706:Methemoglobin / Q000031:analogs & derivatives* / Q000737:chemistry; D008962:Models, Theoretical; D011487:Protein Conformation; D011489:Protein Denaturation; D013816:Thermodynamics
chemical_list: C007169:cyanomethemoglobin; D008706:Methemoglobin