Probing reversible self-association of therapeutic proteins by sedimentation velocity in the analytical ultracentrifuge.
Perez-Ramirez
Bernardo
B
BioFormulations Development, Genzyme, Framingham, MA, USA.
Steckert
John J
JJ
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use; D016466:CHO Cells; D011232:Chemical Precipitation; D006224:Cricetinae; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use*; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use; D014461:Ultracentrifugation / Q000379:methods*
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2