[Protective effects of San Baoxin on injury induced by myocardical ischemic reperfusion and anti-thrombosis in rats].
OBJECTIVE
To investigate the effects of San Baoxin on myocardial injury induced by ischemia-reperfusion (MI/R) and thrombogenesis in rats in vivo and ex vivo.


METHODS
The experimental model was established by ligation of left anterior descending coronary artery for 30 min followed by reperfusion for 180 min. The Chandler method was used to produced ex vivo thrombosis and an electrical stimulation of common carotid artery was adopted to form in vivo thrombosis respectively, the effect of antithrombosis induced by San Baoxin was observed.


RESULTS
San Baoxin significantly decreased the content of malondialdehyde (MDA), obviously elevated the activity of superoxide dismutase (SOD) and increased the amount of NO of the serum simultaneously. The San Baoxin at the dosage of 10 g x kg(-1) could remarkably lengthen the OT ( P < 0.05). All San Baoxin dosages could inhibit the formation of ex vivo thrombosis.


CONCLUSIONS
San Baoxin protects the myocardium from injury of ischemic and reperfusion. The protective effect of San Baoxin may be due to that it can dilate vessels, increase the activity of clearance enzyme of free radical and inhibit lipid peroxidation and the formation of ex vivo and in vivo thrombosis.
Gao
Hui-Li
HL
Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Wang
Yu-Ying
YY
Li
Yi-Kui
YK
Ning
Ke-Yong
KY
Li
Lian-Da
LD
D000818:Animals; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005343:Fibrinolytic Agents / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D017202:Myocardial Ischemia / Q000097:blood / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000097:blood* / Q000473:pathology; D017942:Nitric Acid / Q000097:blood; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood*; D013927:Thrombosis / Q000473:pathology*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005343:Fibrinolytic Agents; D017942:Nitric Acid; D008315:Malondialdehyde; D013482:Superoxide Dismutase