Reactive oxygen scavenging effect of enzymatic extracts from Sargassum thunbergii.
The free radical scavenging activity of water soluble natural antioxidants from Sargassum thunbergii, which is a brown marine alga, was evaluated by examining the radical scavenging activities of the extracts of hydrolyzates from S. thunbergii on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radicals. A spin-trapping electron spin resonance (ESR) spectrometer was employed, and the results were compared for their ESR signal intensity. S. thunbergii was enzymatically hydrolyzed to prepare water soluble extracts by five carbohydrases (AMG, Celluclast, Termamyl, Ultraflo, and Viscozyme) and proteases (Alcalase, Flavorzyme, Kojizyme, Neutrase, and Protamex). The scavenging activity of the radicals increased with increased concentrations of the extracts. The scavenging results were higher for hydroxyl and alkyl radicals and lower for DPPH radical as compared with vitamin C as a reference. The hydrogen peroxide scavenging activity of the extracts was also investigated; the Alcalase extract showed the highest scavenging activity among the extracts prepared with the five proteases and five carbohydrates. In addition, the DNA damage was determined by using the comet assay with alkaline electrophoresis and was quantified by measuring the tail length. The preventive effect of Alcalase extract from S. thunbergii against DNA damage increased with increments of concentration of the enzymatic extracts.
Park
Pyo-Jam
PJ
Department of Biotechnology and Nokyong Research Center, Konkuk University, Chungju 380-701, Korea.
Heo
Soo-Jin
SJ
Park
Eun-Ju
EJ
Kim
Se-Kwon
SK
Byun
Hee-Guk
HG
Jeon
Byong-Tae
BT
Jeon
You-Jin
YJ
D004249:DNA Damage; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D044647:Sargassum / Q000201:enzymology*
D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D006026:Glycoside Hydrolases; C019822:carbohydrase