Growth of human mast cells from bone marrow and peripheral blood-derived CD34+ pluripotent progenitor cells.
Human mast cells (HMCs) are derived from a CD34+ pluripotent progenitor cell that is Kit (CD117+), CD13+, Fc epsilonRI- and lacks lineage-specific surface markers. Bone marrow and peripheral blood are two tissue sources available for obtaining CD34+ progenitor cells from which to culture HMCs. CD34+ cells can be isolated and enriched by magnetic separation columns and stored under specific conditions until ready for use. Alternatively, enriched CD34+ cells may be immediately cultured in serum-free culture media containing recombinant human stem cell factor (rhSCF), rhIL-6, and rhIL-3 (first week only). Weekly hemidepletions and the removal of adherent cells and/or debris enables the investigator to obtain HMC cultures, identified by Wright-Giemsa and acidic toluidine blue stains, by 8-10 wk.
Kirshenbaum
Arnold S
AS
Laboratory of Allergic Diseases, Mast Cell Biology Section, NIH/NIAID, Bethesda, MD, USA.
Metcalfe
Dean D
DD
D018952:Antigens, CD34 / Q000378:metabolism; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D007962:Leukocytes / Q000166:cytology; D008407:Mast Cells / Q000166:cytology / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*
D018952:Antigens, CD34; D015415:Biomarkers