Cloning and characterization of a bile salt hydrolase (bsh) from Bifidobacterium adolescentis.
A gene coding for bile salt hydrolase (BSH) from Bifidobacterium adolescentis was cloned and expressed in Escherichia coli, and the nucleotide sequence was determined. The BSH of E. coli transformants was produced intracellularly in the absence of bile salts. A unique bsh promoter (P(bsh)) sequence was identified by using a Neural Network Promoter Prediction (NNPP, version 2.2). In spite of their high-level sequence homology with other bsh genes in the Bifidobacterium species, their genetic organization surrounding the bsh gene and their promoter sequences are different depending on the species.
Kim
Geun-Bae
GB
Department of Food Science and Agricultural Chemistry, McGill University, Quebec, Canada.
Brochet
Mathieu
M
Lee
Byong H
BH
D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001644:Bifidobacterium / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D024363:Transcription Initiation Site
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000581:Amidohydrolases; C023154:choloylglycine hydrolase