Plasma homocysteine thiolactone adducts associated with risk of coronary heart disease.
BACKGROUND
Homocysteine thiolactone adducts have been proposed as the culprit of homocysteine related cardiovascular diseases. We studied the association of these adducts in plasma, and the gene polymorphism of paraoxonase-2 with coronary heart disease.


METHODS
254 patients and 308 controls were recruited for the study. Homocysteine thiolactone adducts were determined with ELISA. The codon 311 polymorphism of paraoxonase-2 gene was genotyped by using polymerase chain reaction and restrictive digestion.


RESULTS
The plasma level of homocysteine thiolactone adducts were significantly higher in patients than in controls (40.65 +/- 10.87 u/ml vs. 30.58 +/- 10.20 u/ml, P <0.01), with odds ratio of 7.34 (95% confidence interval 4.020-13.406, P <0.01), and increased according to the number of atherosclerotic coronary arteries: 35.59 +/- 10.34 units/ml (n = 76); 41.88 +/- 8.83 (n = 70) and 43.13 +/- 11.47 (n = 108) in subjects with 1, 2 and 3 affected arteries, respectively (r =0.174, P < 0.01). The frequency of CC genotype was significantly higher in patients with coronary heart disease (7.48%) than in controls (1.62%, P < 0.01), with adjusted odds ratio of 4.367 (95% confidence interval: 1.178 to 16.191, P < 0.01), so was the C allele (23.2% vs. 14.9%, P < 0.05).


CONCLUSIONS
High plasma homocysteine thiolactone adducts and the CC 311 genotype of paraoxonase-2 gene may be the emerging risk factor for coronary heart disease.
Yang
Xu
X
Sino-German Laboratory for Molecular Medicine, Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing 100037, PR China.
Gao
Yan
Y
Zhou
Jin
J
Zhen
Yisong
Y
Yang
Yuejin
Y
Wang
Jizheng
J
Song
Li
L
Liu
Yuqing
Y
Xu
Haiyan
H
Chen
Zaijia
Z
Hui
Rutai
R
D000328:Adult; D000483:Alleles; D043303:Aryldialkylphosphatase / Q000235:genetics; D003327:Coronary Disease / Q000097:blood* / Q000201:enzymology / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
D006710:Homocysteine; C007957:homocysteine thiolactone; D043303:Aryldialkylphosphatase