title: Advanced glycosylation end products might promote atherosclerosis through inducing the immune maturation of dendritic cells.
abstract: OBJECTIVE
  Both advanced glycosylation end products (AGEs) and dendritic cells (DCs) have been shown to play a causative role in atherosclerosis. However, whether they function interactively in the process remains uncertain. We therefore studied the effects of AGE-bovine serum albumin (AGE-BSA) on the maturation of DCs and the expressions of scavenger receptor-A (SR-A) and receptor for AGEs (RAGE) on DCs.
  
  
  RESULTS
  AGE-BSA induced DCs maturation accompanied with increased expressions of CD1a, CD40, CD80, CD83, CD86, and MHC class II. The capacity of DCs to stimulate T-cell proliferation and secretion of cytokines (interferon [IFN], IFN-gamma, interleukin [IL]-10 and IL-12) was also enhanced by AGE-BSA. AGE-BSA significantly upregulated SR-A and RAGE expression on DCs and the upregulation was abolished by inhibition of mitogen-activated protein (MAP) kinase Jnk, but not by that of Erk and p38 MAP kinase. AGE-BSA-induced expression of CD83 and secretion of IL-12 were partly inhibited by either an anti-RAGE neutralizing antibody or a Jnk inhibitor.
  
  
  CONCLUSIONS
  AGE-BSA induces maturation of DCs and augmented their capacity to stimulate T-cell proliferation and cytokine secretions possibly through upregulation of RAGE and SR-A, which at least in part through Jnk. These findings might explain in part the interactive roles of AGEs and DCs in the processes of atherosclerosis.
authors.affiliation: Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, 180 Fenglin Rd, Shanghai 200032, People's Republic of China. gejunbo@zshospital.net
authors.forename: Junbo
authors.initials: J
authors.lastname: Ge
authors.forename: Qingzhe
authors.initials: Q
authors.lastname: Jia
authors.forename: Chun
authors.initials: C
authors.lastname: Liang
authors.forename: Yukun
authors.initials: Y
authors.lastname: Luo
authors.forename: Dong
authors.initials: D
authors.lastname: Huang
authors.forename: Aijun
authors.initials: A
authors.lastname: Sun
authors.forename: Keqiang
authors.initials: K
authors.lastname: Wang
authors.forename: Yunzeng
authors.initials: Y
authors.lastname: Zou
authors.forename: Haozhu
authors.initials: H
authors.lastname: Chen
mesh_terms: D000873:Anthracenes / Q000494:pharmacology; D000906:Antibodies / Q000494:pharmacology; D015703:Antigens, CD / Q000378:metabolism; D050197:Atherosclerosis / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D002450:Cell Communication / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology*; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D066298:In Vitro Techniques; D018664:Interleukin-12 / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism; D009000:Monocytes / Q000166:cytology; D000067759:Receptor for Advanced Glycation End Products; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051117:Scavenger Receptors, Class A / Q000235:genetics / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology; D000097802:CD83 Antigen
chemical_list: D000873:Anthracenes; D000906:Antibodies; D015703:Antigens, CD; D017127:Glycation End Products, Advanced; D007136:Immunoglobulins; D018664:Interleukin-12; D048031:JNK Mitogen-Activated Protein Kinases; D008562:Membrane Glycoproteins; D000067759:Receptor for Advanced Glycation End Products; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D051117:Scavenger Receptors, Class A; D012710:Serum Albumin, Bovine; D000097802:CD83 Antigen; C081628:advanced glycation end products-bovine serum albumin; C432165:pyrazolanthrone