title: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: Major strengths of mass spectrometry analysis include the accuracy of the detection principle and the automatic data storage, making it a premier choice for high-throughput single-nucleotide polymorphism genotyping. We explain the assay principle in detail and give step-by-step laboratory instructions. Finally, references point toward further use of mass spectrometry analysis for molecular haplotyping, pooled DNA analysis, re-sequencing, and gene expression studies.
authors.affiliation: Molecular Epidemiology, GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg/Munich, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Wjst
authors.forename: Dirk
authors.initials: D
authors.lastname: van den Boom
mesh_terms: D001331:Automation; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*