Human pescadillo induces large-scale chromatin unfolding.
The human pescadillo gene encodes a protein with a BRCT domain. Pescadillo plays an important role in DNA synthesis, cell proliferation and transformation. Since BRCT domains have been shown to induce chromatin large-scale unfolding, we tested the role of Pescadillo in regulation of large-scale chromatin unfolding. To this end, we isolated the coding region of Pescadillo from human mammary MCF10A cells. Compared with the reported sequence, the isolated Pescadillo contains in-frame deletion from amino acid 580 to 582. Targeting the Pescadillo to an amplified, lac operator-containing chromosome region in the mammalian genome results in large-scale chromatin decondensation. This unfolding activity maps to the BRCT domain of Pescadillo. These data provide a new clue to understanding the vital role of Pescadillo.
Zhang
Hao
H
Beijing Institute of Biotechnology, Beijing 100850, China.
Fang
Yan
Y
Huang
Cuifen
C
Yang
Xiao
X
Ye
Qinong
Q
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D016466:CHO Cells; D002460:Cell Line; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011506:Proteins / Q000235:genetics* / Q000502:physiology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014162:Transfection
D002843:Chromatin; D018076:DNA, Complementary; C484463:PES1 protein, human; D011506:Proteins; D016601:RNA-Binding Proteins; D012313:RNA