Premature sister chromatid separation in HIV-1-infected peripheral blood lymphocytes.
To investigate the mechanism of aneuploidy that is frequently observed in AIDS, we examined premature sister chromatid separation (PCS), a sign of genomic instability, in peripheral blood cells of HIV-1-infected individuals. PCS was found in all six HIV-1 individuals at a high incidence. When peripheral blood cells from healthy volunteers were infected with HIV-1 in vitro, the incidence of PCS increased. This suggests that HIV-1 infection causes PCS and has the potential to induce aneuploidy.
Shimura
Mari
M
Department of Intractable Diseases, Research Institute, International Medical Center of Japan, Shinjuku-ku, Tokyo 162-8655, Japan .
Tokunaga
Kenzo
K
Konishi
Mitsuru
M
Sato
Yuko
Y
Kobayashi
Chizuko
C
Sata
Tetsutaro
T
Ishizaka
Yukihito
Y
D000782:Aneuploidy; D002478:Cells, Cultured; D002842:Chromatids / Q000235:genetics*; D020090:Chromosome Segregation / Q000235:genetics; D015658:HIV Infections / Q000097:blood / Q000235:genetics* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000821:virology*; D008677:Metaphase; D012367:RNA, Viral / Q000097:blood
D012367:RNA, Viral