Platelet release of beta-thromboglobulin and platelet factor 4 and serotonin in plasma samples.
OBJECTIVE
Platelet release of alpha granule-derived CXC chemokines and dense granule-derived serotonin in plasma samples was evaluated.


METHODS
Concentrations of the CXC chemokines beta-TG and PF4 were assayed by an enzyme immunoassay; serotonin was measured by an HPLC method.


RESULTS
Beta-TG and PF4 were more easily released than serotonin by in vitro procedures. Use of the anti-platelet cocktail CTAD and preservation of the samples at 4 degrees C were necessary to accurately measure beta-TG and PF4, but not serotonin.


CONCLUSIONS
Assaying serotonin may be useful for assessing platelet activation in vivo as a laboratory test because of facile preparation of plasma samples.
Ohkawa
Ryunosuke
R
Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.
Hirowatari
Yuji
Y
Nakamura
Kazuhiro
K
Ohkubo
Shigeo
S
Ikeda
Hitoshi
H
Okada
Mitsumasa
M
Tozuka
Minoru
M
Nakahara
Kazuhiko
K
Yatomi
Yutaka
Y
D000241:Adenosine / Q000494:pharmacology; D000328:Adult; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D019743:Chemokines, CXC / Q000097:blood / Q000378:metabolism*; D019343:Citric Acid / Q000494:pharmacology; D004176:Dipyridamole / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D015539:Platelet Activation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D010978:Platelet Factor 4 / Q000378:metabolism*; D012701:Serotonin / Q000097:blood / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology; D001620:beta-Thromboglobulin / Q000378:metabolism*
D019743:Chemokines, CXC; D010975:Platelet Aggregation Inhibitors; D001620:beta-Thromboglobulin; D019343:Citric Acid; D012701:Serotonin; D010978:Platelet Factor 4; D004176:Dipyridamole; D004492:Edetic Acid; D013806:Theophylline; D000241:Adenosine