title: [Establishment of reporter gene-based assays for the identification of (anti) androgenic effects of chemicals in CHO cells].
abstract: OBJECTIVE
  Develop reporter gene-based assays for the identification of (anti) androgenic effects of chemicals in CHO cells, explore the antiandrogenic effects of 2,4-DDT and methoxychlor.
  
  
  METHODS
  Chinese Ovary cells were cotransfected with the human androgen receptor expression vector, mouse mammary tumour virus MMTV-luciferase vector and phRL-SV40 using the transfection reagent Sofast, test the luciferase activity. After treatment of the cells for 24 h with dihydrotestosterone (DHT), flutamide (FLU), validate the effectiveness and specificity, also test the effect of 2,4-DDT and methoxychlor (METH).
  
  
  RESULTS
  DHT is a potent agonist, 1 x 10(-10) mol/L DHT could result in the enhancement of luciferase activity, FLU is a complete antagonist, that confirmed the effectiveness and specificity of test system. 2,4-DDT and METH are partial antagonists. 3 x 10(-7) mol/L and above 2,4-DDT and METH could inhibit the androgenic activity of DHT.
  
  
  CONCLUSIONS
  The assay we developed is doful, 2,4-DDT and METH could antagonize the androgenic effects.
authors.affiliation: Department of Toxicology, Second Military Medical University, Shanghai 200433, China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Zhao
authors.forename: Jiang-Bo
authors.initials: JB
authors.lastname: Zhu
authors.forename: Yong-Fei
authors.initials: YF
authors.lastname: Zhu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Xili
authors.initials: X
authors.lastname: Ma
authors.forename: Tianbao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000726:Androgen Antagonists / Q000633:toxicity*; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003634:DDT / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity*; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008731:Methoxychlor / Q000633:toxicity*; D014162:Transfection
chemical_list: D000726:Androgen Antagonists; D052244:Endocrine Disruptors; D003634:DDT; C016340:o,p'-DDT; D008156:Luciferases; D008731:Methoxychlor