A preliminary cinemicrographic study on the effect of NGF on the growth of chick embryonic sensory neurites in vitro.
Trypsin dissociated dorsal root ganglia of 8-day-old chick embryos were cultured and filmed in the presence or absence of NGF. The behaviour of neurites and their association with Schwann cells and fibroblasts were observed and described. Isolated neurons cultured in the presence or absence of NGF were filmed and quantitatively analysed to determine the rate of increase in length of their neurites. Isolated neurons growing in control medium were perfused with either NGF or Medium 199 to determine the effect these agents had on rate of advance of nerve processes. Under these particular experimental conditions NGF was shown to increase the rate of growth in length of embryonic neurites but no more than did the nutrient Medium 199.
Blood
L A
LA
School of Anatomy, University of Queensland, St. Lucia, Australia.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology / Q000196:embryology; D008853:Microscopy; D009040:Motion Pictures; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure
D003470:Culture Media; D020932:Nerve Growth Factor