title: Nogo-66 receptor at cerebellar cortical glia gap junctions in the rat.
abstract: Nogo-A is a myelin inhibitor of neurite outgrowth that accounts for the difficulty in fiber regeneration in the central nervous system. Its 66-amino-acid extracellular domain (Nogo-66) contributes to the inhibitory activity of Nogo-A. The Nogo-66 receptor is widely distributed in neurons of the central nervous system, including the cerebellum. In our study on the distribution of Nogo-66 receptor in the cerebellar cortex in the rat, we unexpectedly found Nogo-66 receptor immunoreactivity in the glia cells, particularly abundant beneath the Purkinje cells. The presence of Nogo-66 receptor in glia cells has not been reported before. A detailed study was thus conducted. Immunoelectron microscopic investigation clearly demonstrated that the Nogo-66 receptor immunoreactivity could be ascertained at the gap junction between glia cells, indicating that the Nogo-66 receptor may modulate the communication between glia cells through gap junctions.
authors.affiliation: Institute of Neurosciences, 4th Military Medical University, Xi'an, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Liu
authors.forename: Wei-Lin
authors.initials: WL
authors.lastname: Jin
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Liu
authors.forename: Gong
authors.initials: G
authors.lastname: Ju
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002525:Cerebellar Cortex / Q000166:cytology*; D005455:Fluorescent Antibody Technique / Q000379:methods; D058851:GPI-Linked Proteins; D017629:Gap Junctions / Q000378:metabolism* / Q000648:ultrastructure; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000379:methods; D009185:Myelin Proteins / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology* / Q000648:ultrastructure; D000070816:Nogo Receptor 1; D009842:Oligopeptides / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D058851:GPI-Linked Proteins; D005904:Glial Fibrillary Acidic Protein; D009185:Myelin Proteins; D000070816:Nogo Receptor 1; D009842:Oligopeptides; C111711:RNAIII inhibiting peptide; D011956:Receptors, Cell Surface; C486581:Rtn4r protein, rat