Cell sorting by one gravity SPLITT fractionation.
The need for innovative separative techniques suitable for the fractionation of biomaterials prompted this investigation into the performance of the gravitational split-flow thin channel (G-SPLITT) system as a cell sorter. The rigorous mathematical description of the separation mechanism allows achievement of fast separation of several million myeloma cells from healthy splenocytes using flow conditions calculated from theory. Separation in G-SPLITT is based on differences in sedimentation rate. For accurate prediction of the optimal working conditions, this parameter was directly measured by cell tracking velocimetry rather than relying on a measure of diameter (by Multisizer) and an assumed density for each cell population. We also discuss the influence of different flow conditions on the effectiveness of separation.
Benincasa
Maria-Anna
MA
Department of Chemistry, University of Rome La Sapienza, Piazzale A. Moro 5, 00185 Rome, Italy. mariaanna.benincasa@uniroma1.it
Moore
Lee R
LR
Williams
P Stephen
PS
Poptic
Earl
E
Carpino
Francesca
F
Zborowski
Maciej
M
D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D031521:Fractionation, Field Flow / Q000295:instrumentation* / Q000379:methods*; D006112:Gravitation; D008545:Melanoma; D013154:Spleen