title: Thermodynamic stability of RNA structures formed by CNG trinucleotide repeats. Implication for prediction of RNA structure.
abstract: Trinucleotide repeat expansion diseases (TREDs) are correlated with elongation of CNG DNA and RNA repeats to pathological level. This paper shows, for the first time, complete data concerning thermodynamic stabilities of RNA with CNG trinucleotide repeats. Our studies include the stability of oligoribonucleotides composed of two to seven of CAG, CCG, CGG, and CUG repeats. The thermodynamic parameters of helix propagation correlated with the presence of multiple N-N mismatches within CNG RNA duplexes were also determined. Moreover, the total stability of CNG RNA hairpins, as well as the contribution of trinucleotide repeats placed only in the stem or loop regions, was evaluated. The improved thermodynamic parameters allow to predict much more accurately the thermodynamic stabilities and structures of CNG RNAs.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznañ, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Broda
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Kierzek
authors.forename: Zofia
authors.initials: Z
authors.lastname: Gdaniec
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Kulinski
authors.forename: Ryszard
authors.initials: R
authors.lastname: Kierzek
mesh_terms: D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D020871:RNA Stability; D013816:Thermodynamics; D018911:Trinucleotide Repeats
chemical_list: D012313:RNA