A sericin-derived peptide protects sf9 insect cells from death caused by acute serum deprivation.
Sericin is the silk protein enveloping fibroin fibers in cocoons. Sericin hydrolysate protects cultured Sf9 insect cells from death caused by serum deprivation; the activity depends on the repeats of 38 amino acids. A partial peptide from the 38 residues, SGGSSTYGYS, inhibited serum-deprivation death as well. Cell viabilities in the presence of 10% (v/v) foetal calf serum, no additives and 1 mM: SGGSSTYGYS were 96, 12 and 31% on the third day after inoculation, respectively. Aromatic residues seemed to be important because SGGSSTWGWS had the same activity as SGGSSTYGYS but SGGSSTAGAS had no activity.
Takahashi
Masakazu
M
Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjojima, 910-1195 Fukui, Japan. mastak@fpu.ac.jp
Tsujimoto
Kazuhisa
K
Kato
Youichi
Y
Yamada
Hideyuki
H
Takagi
Hiroshi
H
Nakamori
Shigeru
S
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology*; D047030:Sericins / Q000737:chemistry / Q000494:pharmacology*; D018411:Spodoptera / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
D016895:Culture Media, Serum-Free; D010446:Peptide Fragments; D047030:Sericins