title: General metabolism of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis.
abstract: Annotation of the transcriptome of the dimorphic fungus Paracoccidioides brasiliensis has set the grounds for a global understanding of its metabolism in both mycelium and yeast forms. This fungus is able to use the main carbohydrate sources, including starch, and it can store reduced carbons in the form of glycogen and trehalose; these provide energy reserves that are relevant for metabolic adaptation, protection against stress and infectivity mechanisms. The glyoxylate cycle, which is also involved in pathogenicity, is present in this fungus. Classical pathways of lipid biosynthesis and degradation, including those of ketone body and sterol production, are well represented in the database of P. brasiliensis. It is able to synthesize de novo all nucleotides and amino acids, with the sole exception of asparagine, which was confirmed by the fungus growth in minimal medium. Sulfur metabolism, as well as the accessory synthetic pathways of vitamins and co-factors, are likely to exist in this fungus.
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, 70910-900 Brasília, DF, Brazil.
authors.forename: Fabrício B M
authors.initials: FB
authors.lastname: Arraes
authors.forename: Bruno
authors.initials: B
authors.lastname: Benoliel
authors.forename: Rafael T
authors.initials: RT
authors.lastname: Burtet
authors.forename: Patrícia L N
authors.initials: PL
authors.lastname: Costa
authors.forename: Alexandro S
authors.initials: AS
authors.lastname: Galdino
authors.forename: Luanne H A
authors.initials: LH
authors.lastname: Lima
authors.forename: Camila
authors.initials: C
authors.lastname: Marinho-Silva
authors.forename: Luciana
authors.initials: L
authors.lastname: Oliveira-Pereira
authors.forename: Pollyanna
authors.initials: P
authors.lastname: Pfrimer
authors.forename: Luciano
authors.initials: L
authors.lastname: Procópio-Silva
authors.forename: Viviane Castelo-Branco
authors.initials: VC
authors.lastname: Reis
authors.forename: Maria Sueli S
authors.initials: MS
authors.lastname: Felipe
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D050260:Carbohydrate Metabolism; D020224:Expressed Sequence Tags / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D010228:Paracoccidioides / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011687:Purines / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D011687:Purines; D011743:Pyrimidines; D013455:Sulfur