Effects of dietary n-6 or n-3 polyunsaturated fatty acids protected or not against ruminal hydrogenation on plasma lipids and their susceptibility to peroxidation in fattening steers.
Two experiments were conducted using crossbred Salers x Charolais fattening steers fed diets enriched with no supplemental oilseeds or oils rich in either n-6 PUFA (from sunflower seeds) or n-3 PUFA (from linseeds) provided either as seeds incorporated in the diet (i.e., not protected from ruminal bacterial hydrogenation) or by chronic infusion into the duodenum (protected form). In the Sunflower experiment, animals (initial age = 454 +/- 20 d; initial BW = 528 +/- 36 kg) received a control diet for 70 d (CS, n = six) consisting of hay and concentrate, or the same basal diet supplemented with sunflower oil (4% of dietary DM), either fed as seeds (SS, n = six) or infused into the duodenum (ISO, n = six). The same experimental design was applied to animals (initial age = 412 +/- 33 d; initial BW = 536 +/- 33 kg) used in the Linseed experiment (CL, LS, and ILO; n = 8 per group). For all animals, blood was sampled every 15 d during 70 d. In both trials, a significant diet x time interaction (P < 0.001) was detected for plasma concentrations of apolipoprotein A-I, phospholipids, and free and esterified cholesterol, with values increasing with time during administration of the PUFA-rich diets being more evident with ISO and ILO diets. Plasma fatty acids were altered with oil infusions, with increased concentrations of n-6 (1.6-fold; P < 0.05) and n-3 PUFA (4.5-fold; P < 0.05) and of their respective indicies of peroxidizability (1.2- and 1.5-fold with Diets ISO and ILO, respectively; P < 0.05). In vitro copper-induced peroxidation of lipids revealed a decreased length of the lag phase in the process of conjugated diene generation by 48% (P < 0.005) with the ILO diet, indicating less resistance against peroxidation than in control steers. Compared with CS, the ISO treatment increased plasma alpha-tocopherol (x2.5; P < 0.05) leading to similar resistance against peroxidation. After depletion of this vitamin, the rates of peroxidation and production of conjugated dienes were greater (twofold; P < 0.05) with the ISO and ILO diets than with the others. In conclusion, infusion of sunflower or linseed oil into the duodenum altered the composition and distribution of plasma lipids and increased the plasma concentration of PUFA. The sensitivity of plasma PUFA to peroxidation depends on the plasma level of antioxidants, especially vitamin E, a nutrient important both for the health of animals and for the stability of the blood lipids until their tissue deposit.
Scislowski
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V
INRA, Research Unit on Herbivores, Nutrients and Metabolisms Group, Research Centre of Clermont-Ferrand/Theix, Saint Genès-Champanelle, France.
Bauchart
D
D
Gruffat
D
D
Laplaud
P M
PM
Durand
D
D
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002417:Cattle / Q000097:blood / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D002839:Chromans / Q000097:blood; D004032:Diet / Q000662:veterinary; D004386:Duodenum / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000097:blood; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D006865:Hydrogenation; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D010949:Plasma / Q000378:metabolism; D011897:Random Allocation; D012417:Rumen / Q000378:metabolism* / Q000382:microbiology; D013997:Time Factors; D024502:alpha-Tocopherol / Q000097:blood
D002839:Chromans; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D002784:Cholesterol; D024502:alpha-Tocopherol; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid