title: The yliA, -B, -C, and -D genes of Escherichia coli K-12 encode a novel glutathione importer with an ATP-binding cassette.
abstract: Glutathione protects cells and organisms from oxygen species and peroxides and is indispensable for aerobically living organisms. Moreover, it acts against xenobiotics and drugs by the formation and excretion of glutathione S conjugates. In this study, we show that the yliA, -B, -C, and -D genes of Escherichia coli K-12 encode a glutathione transporter with the ATP-binding cassette. The transporter imports extracellular glutathione into the cytoplasm in an ATP-dependent manner. This transporter, along with gamma-glutamyltranspeptidase, has an important role in E. coli growth with glutathione as a sole sulfur source.
authors.affiliation: Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan. hideyuki@lif.kyoto-u.ac.jp
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Takashi
authors.initials: T
authors.lastname: Koyanagi
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Izuka
authors.forename: Akiko
authors.initials: A
authors.lastname: Onishi
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Kumagai
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D003593:Cytoplasm / Q000378:metabolism; D017931:DNA Primers; D048168:Escherichia coli K12 / Q000235:genetics* / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D018698:Glutamic Acid