Flow injection chemiluminescence determination of isoniazid using the lucigenin-periodate system.
A weak chemiluminescence (CL) signal was observed during the mixing of isoniazid with lucigenin in alkaline aqueous solution. The CL signal was enhanced more than 100 times in the presence of potassium periodate. This CL system was developed for the determination of isoniazid using a flow injection mode. The CL intensity is proportional to the concentration of isoniazid in the range 0.005-1.0 mg/L. The limit of detection is 0.0034 mg/L and the relative standard deviation is 2.0% for 0.2 mg/L isoniazid solution in 11 repeated measurements. The method was applied to the determination of isoniazid in pharmaceutical preparations and satisfactory results were obtained.
Du
Jianxiu
J
School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China. jxdu@snnu.edu.cn
Lu
Jiuru
J
D000166:Acridines / Q000737:chemistry*; D000995:Antitubercular Agents / Q000032:analysis*; D017022:Flow Injection Analysis / Q000379:methods*; D007538:Isoniazid / Q000032:analysis*; D008163:Luminescent Measurements; D016877:Oxidants / Q000737:chemistry; D010504:Periodic Acid / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry
D000166:Acridines; D000995:Antitubercular Agents; D016877:Oxidants; D013501:Surface-Active Agents; D010504:Periodic Acid; C033472:10,10'-dimethyl-9,9'-biacridinium; D007538:Isoniazid