title: [Specific killing effects of adenovirus-mediated double suicide gene driven by KDR promoter on venous endothelial cells and gastric cancer cells in vitro].
abstract: OBJECTIVE
  To study the specific killing effect of adenovirus(Ad)-mediated double suicide gene under regulation by KDR promoter on gastric cancer cells and venous endothelial cells in vitro.
  
  
  METHODS
  KDR-expressing MGC803 and ECV304 cells and non-KDR-expressing LS174T cells were infected by the two Ads (AdEasy-KDR-CDglyTK and AdEasy-CMV- CDglyTK), and expression of CDglyTK was detected by reverse transcriptional (RT) PCR. After treatment with 5-FC and GCV, the killing effects of the double suicide genes on various cells were evaluated.
  
  
  RESULTS
  The infection rate of the two resultant recombinant Ads did not differ significantly in the cells. RT-PCR demonstrated the presence of CDglyTK gene product in all the cells infected by Ad-CMV-CDglyTK and all but SL147T cells infected by Ad-KDR-CDglyTK. All the cells infected by Ad-CMV-CDglyTK and ECV304 and MGC803 cells infected Ad-KDR-CDglyTK were highly sensitive to the prodrugs. In contrast, LS174T cells infected by Ad-KDR-CDglyTK did not appear sensitive to the two prodrugs (P<0.001). In addition, the effect of the double suicide gene was much stronger than that of either of the single suicide gene (P<0.001), showing also considerable bystander effect.
  
  
  CONCLUSIONS
  The double suicide gene driven by KDR promoter has specific killing effect on KDR-expressing gastric tumor cells and venous endothelial cells in vitro.
authors.affiliation: Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Zong-hai
authors.initials: ZH
authors.lastname: Huang
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Su
authors.forename: Jin-long
authors.initials: JL
authors.lastname: Yu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Li
authors.forename: Ying-fang
authors.initials: YF
authors.lastname: Fan
authors.forename: Guang-jun
authors.initials: GJ
authors.lastname: Zhou
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Song
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D043525:Cytosine Deaminase / Q000235:genetics; D042783:Endothelial Cells / Q000166:cytology*; D041722:Genes, Transgenic, Suicide / Q000235:genetics*; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D011401:Promoter Regions, Genetic; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D011993:Recombinant Fusion Proteins; D020794:Receptor Protein-Tyrosine Kinases; D043525:Cytosine Deaminase