title: Activation of monoamine oxidase isotypes by prolonged intake of aluminum in rat brain.
abstract: Rats were fed 100 microM aluminum maltolate for one year in their drinking water. Brain aluminum contents have increased 4.2-fold in the aluminum-treated group, whereas no significant changes in the body weight, brain weight, and brain protein content were observed. Long-term aluminum feeding induced apoptosis as assessed by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method and showed activatory effects on the catalytic efficiency (kcat/KM) of monoamine oxidase-A and monoamine oxidase-B up to 1.9- and 3.8-fold, respectively. The expression level of monoamine oxidase isotypes on the Western blot remained unchanged between the two groups, suggesting a change in post-translational regulation of the activities of monoamine oxidase isotypes by long-term aluminum feeding.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, 388-1 Poongnap-dong, Songpa-gu, Seoul 138-736, Republic of Korea.
authors.forename: Jae-Wan
authors.initials: JW
authors.lastname: Huh
authors.forename: Myung-Min
authors.initials: MM
authors.lastname: Choi
authors.forename: Jang Han
authors.initials: JH
authors.lastname: Lee
authors.forename: Seung-Ju
authors.initials: SJ
authors.lastname: Yang
authors.forename: Mi Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Jene
authors.initials: J
authors.lastname: Choi
authors.forename: Kwan Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Jong Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
mesh_terms: D000284:Administration, Oral; D000535:Aluminum / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology; D004789:Enzyme Activation; D020869:Gene Expression Profiling; D008297:Male; D008995:Monoamine Oxidase / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000535:Aluminum; D008995:Monoamine Oxidase