Actinic light-energy dependence of proton release from bacteriorhodopsin.
Measuring the light-density (fluence) dependence of proton release from flash excited bacteriorhodopsin with two independent methods we found that the lifetime of proton release increases and the proton pumping activity, defined as a number of protons per number of photocycle, decreases with increasing fluence. An interpretation of these results, based on bending of purple membrane and electrical interaction among the proton release groups of bacteriorhodopsin trimer, is presented.
Tóth-Boconádi
R
R
Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
Taneva
S G
SG
Keszthelyi
L
L
D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D018499:Linear Energy Transfer; D017494:Proton Pumps / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage; D014466:Ultraviolet Rays
D017494:Proton Pumps; D001436:Bacteriorhodopsins