[The determination of echinacoside and acteoside in herbs of Cistanche tubulosa].
OBJECTIVE
To analyse the echinacoside and acteoside content of Cistanche tubulosa, collected from different hosts and different size of the cultivated, which is compared to the wilding by RP-HPLC method.


METHODS
An Agilent Eclipse XDB-C18(4.6 mm x 250 mm, 5 microm) column was used and a mixture of methanol-acetonitrile-1% acetic acid (15:10:75) was used as the mobile phase at a flow rate of 0.6 mL x min(-1). The column temperature was 30 degrees C and the UV detection wavelength was 334 nm.


RESULTS
The calibration curves of echinacoside and acteoside were in good linearity over the range of 0.904-9.04 microg (r = 0.999 9), and 1.27-12.7 microg (r = 0.999 9) respectively and the average recoveries of echinacoside (and acteoside) were 98.9% (n = 5, RSD 1.9%), and 97.0% (n = 5, RSD 0.97%).


CONCLUSIONS
The method is simple, quick, acurate. In all of the samples, the contents of echinacoside is markedly more than that of acteoside, the content of the two active component in the wilding is higher than that in the planting. The content of sample in the different sizes gradually increase from the big to the small, and the contents of samples collected from the different hosts vary markedly. These results are useful for the quality evaluation of medicinal materials of C. tubulosa.
Chen
Min
M
Institute of Chinese Materia Medica, Chinese Academy of Traditional Chinese Medicine, Beijing 100700, China.
Xiao
Su-Ping
SP
Cui
Guang-Hong
GH
Zhang
Si-Ju
SJ
Wu
Zhi-Gang
ZG
Huang
Lu-Qi
LQ
Liu
Ming-Ting
MT
D001947:Breeding; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031671:Cistanche / Q000033:anatomy & histology / Q000737:chemistry* / Q000502:physiology; D005960:Glucosides / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D006790:Host-Parasite Interactions; D010636:Phenols / Q000032:analysis*; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000737:chemistry* / Q000502:physiology; D032363:Tamaricaceae / Q000145:classification / Q000469:parasitology; D059808:Polyphenols
D005960:Glucosides; D006027:Glycosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols; C060297:echinacoside