Toxicity of tire debris extracts on human lung cell line A549.
TD, produced by tire wear, is a significant constituent of PM(10) in urban areas where traffic related emissions are predominant. TD contains a lot of chemicals which can affect human respiratory system and it has received little attention until now, even the toxicity of PM has been extensively documented. A549 cells, a human alveolar lung cells, were exposed for 24, 48, 72 h to 10, 50, 60, 75 microg/ml of TD organic extract. MTT and Trypan Blue assays were used to evaluate cytotoxicity and Comet Assay to evidence DNA damage. TD extracts induced a dose-dependent increase in cell mortality and DNA damage. A significant toxicity was observed when cells were exposed to 60 microg/ml for 72 h. Moreover cell morphology observed at ultra structural level, was severely affected at the highest dose.
Gualtieri
Maurizio
M
Department of Environmental Science, University of Milano-Bicocca, Piazza della Scienza 1, 20126 Milano, Italy.
Rigamonti
Laura
L
Galeotti
Valentina
V
Camatini
Marina
M
D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D006801:Humans; D010316:Particle Size; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000473:pathology; D012408:Rubber / Q000633:toxicity*; D013997:Time Factors
D004785:Environmental Pollutants; D012408:Rubber