title: Transposon tools and methods in zebrafish.
abstract: Zebrafish is an excellent model animal to study vertebrate development by genetic approaches. Hundreds of mutations affecting various processes of development have been isolated by chemical mutagenesis and insertional mutagenesis using a pseudotyped retrovirus. However, useful transposon tools and methods had not been available in zebrafish. This is mainly because no active transposable element has been found from the zebrafish genome. Recently, efficient transgenesis, gene trap, and enhancer trap methods have been developed in zebrafish by using the Tol2 and the Sleeping Beauty transposon systems. These methods should increase the usefulness of zebrafish as a model vertebrate and facilitate the study of developmental biology, genetics, and genomics.
authors.affiliation: Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan. kokawaka@lab.nig.ac.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004251:DNA Transposable Elements; D004742:Enhancer Elements, Genetic; D005091:Exons; D005821:Genetic Techniques; D016678:Genome; D023281:Genomics; D008957:Models, Genetic; D016296:Mutagenesis; D016254:Mutagenesis, Insertional; D015027:Zebrafish
chemical_list: D004251:DNA Transposable Elements