Affordable, abbreviated roche monitor assay for quantification of human immunodeficiency virus type 1 RNA in plasma.
The cost for the Roche Monitor assay kit can be reduced 50% by using only the 1:1, 1:25, and 1:625 human immunodeficiency virus (HIV) and the 1:1 quantitation standard dilutions. This abbreviated test applied to 1,774 mostly African samples had results nearly identical to those obtained following the package insert instructions. To make this approach feasible, Roche would have to provide additional lysis buffer and master mix.
Fiscus
Susan A
SA
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. fiscussa@med.unc.edu
Chen
Shu
S
Hoover
Donald
D
Kerkau
Melissa G
MG
Alabanza
Paul
P
Siharath
Som
S
Schmitz
John
J
Kumwenda
Newton
N
Taha
Taha E
TE
D005260:Female; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D011247:Pregnancy; D011446:Prospective Studies; D012367:RNA, Viral / Q000097:blood*; D011933:Reagent Kits, Diagnostic / Q000191:economics*; D019562:Viral Load
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic