title: Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells.
abstract: OBJECTIVE
  To evaluate the cytotoxic effects of two brands of mineral trioxide aggregate (MTA) (Pro-Root MTA and MTA Angelus) and Portland cement (PC) on the human ECV 304 endothelial cell line.
  
  
  METHODS
  Endothelial ECV 304 cells were incubated at 37 degrees C in an atmosphere of 95% air, 5% carbon dioxide and 100% humidity for 7 days and grown in F12 medium supplemented with 10% fetal bovine serum with 50 microg mL(-1) of gentamicin sulphate. Effects of the materials on mitochondrial functions were measured by a colorimetric assay. At each experimental time interval (24, 48 and 72 h), a dimethyl-thiazol-diphenyl tetrazolium bromid assay was conducted to measure cell viability. All assays were repeated three times to ensure reproducibility. Results were expressed as average absorbance (A(570/nm)) +/- SD and the data were analysed statistically by one-way analysis of variance and the Bonferroni post-test. A P-value < 0.05 was considered statistically significant.
  
  
  RESULTS
  No statistically significant difference was shown between any of the experimental materials (P > 0.05).
  
  
  CONCLUSIONS
  The two brands of MTA analysed, as well as the PC, initially showed a similar elevated cytotoxic effect that decreased gradually with time allowing the cell culture to become reestablished.
authors.affiliation: Department of Endodontics, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil. endogus@gmail.com
authors.forename: G
authors.initials: G
authors.lastname: De Deus
authors.forename: R
authors.initials: R
authors.lastname: Ximenes
authors.forename: E D
authors.initials: ED
authors.lastname: Gurgel-Filho
authors.forename: M C
authors.initials: MC
authors.lastname: Plotkowski
authors.forename: T
authors.initials: T
authors.lastname: Coutinho-Filho
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity; D000704:Analysis of Variance; D017610:Calcium Compounds / Q000633:toxicity; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents / Q000378:metabolism; D003738:Dental Cements / Q000633:toxicity; D004338:Drug Combinations; D042783:Endothelial Cells / Q000187:drug effects*; D006801:Humans; D016014:Linear Models; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010087:Oxides / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004396:Coloring Agents; D003738:Dental Cements; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; C472247:accelerated Portland cement; C086631:mineral trioxide aggregate; C022616:thiazolyl blue