Antitumor effect of cotylenin A plus interferon-alpha: possible therapeutic agents against ovary carcinoma.
OBJECTIVE
Recently, we found that cotylenin A and IFNalpha synergistically inhibited growth both in vitro and in vivo, and induced apoptosis in human cancer cells. For the clinical application of this combined treatment, suitable cancer targets should be selected.


METHODS
We examined the combined effects of these compounds on various types of cancer cells by a human cancer cell line panel assay, and on cancer cells that had been freshly isolated from patients in three-dimensional cultures embedded in collagen gel.


RESULTS
In the analysis of 39 cancer cell lines, ovarian cancer cells were highly sensitive to combined treatment with cotylenin A and IFNalpha in inhibiting cell growth. This treatment was also effective toward ovarian cancer cells that were refractory to CDDP, and significantly inhibited the growth of ovarian cancer cells as xenografts without apparent adverse effects. Ovarian cancer cells from the patients were also sensitive to the combined treatment in primary cultures.


CONCLUSIONS
Combined treatment with cotylenin A and IFNalpha may have therapeutic value in treating ovarian cancer.
Honma
Yoshio
Y
Saitama Cancer Center Research Institute, Saitama, and Department of Life Science, Shimane University Faculty of Medicine, Izumo, Japan. biohonma@med.shimane-u.ac.jp
Kasukabe
Takashi
T
Yamori
Takao
T
Kato
Nobuo
N
Sassa
Takeshi
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D045744:Cell Line, Tumor; D003094:Collagen; D004224:Diterpenes / Q000008:administration & dosage / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
D004224:Diterpenes; D016898:Interferon-alpha; C423222:cotylenin A; D003094:Collagen