[Effects of methyl jasmonate and salicylic acid on phenylethanoid glycosides synthesis in suspension cultures of Cistanche deserticola].
The present study investigated the influence of the methyl jasmonate and salicylic acid elicitors on the formation of phenylethanoid glycosides (PeG) in the suspension cultures of Cistanche deserticola. The results showed that methyl jasmonate and salicylic acid enhanced greatly the accumulation of PeG and echinacoside (Echin), but their optimum elicitation dosage and addition time were different. The yields of PeG and Echin were significantly increased in the presence of 5 micromol/L methyl jasmonate on day 14 (up to 2.59-fold and 3.82-fold, respectively), whereas treated with 50 micromol/L salicylic acid on day 28, the maximum content of them were, respectively, 2.71 and 3.16-fold higher than the untreated cell cultures.
Xu
Liang-Sheng
LS
Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Xue
Xiao-Feng
XF
Fu
Chun-Xiang
CX
Jin
Zhi-Ping
ZP
Chen
Yu-Quan
YQ
Zhao
De-Xiu
DX
D000085:Acetates / Q000494:pharmacology*; D018929:Cell Culture Techniques; D031671:Cistanche / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D003517:Cyclopentanes / Q000494:pharmacology*; D006027:Glycosides / Q000096:biosynthesis*; D054883:Oxylipins / Q000494:pharmacology*; D010626:Phenylethyl Alcohol / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology*
D000085:Acetates; D003470:Culture Media; D003517:Cyclopentanes; D006027:Glycosides; D054883:Oxylipins; C072239:methyl jasmonate; D010626:Phenylethyl Alcohol; D020156:Salicylic Acid