Synergistic defect in 60S ribosomal subunit assembly caused by a mutation of Rrs1p, a ribosomal protein L11-binding protein, and 3'-extension of 5S rRNA in Saccharomyces cerevisiae.
Rrs1p, a ribosomal protein L11-binding protein, has an essential role in biogenesis of 60S ribosomal subunits. We obtained conditionally synthetic lethal allele with the rrs1-5 mutation and determined that the mutation is in REX1, which encodes an exonuclease. The highly conserved leucine at 305 was substituted with tryptophan in rex1-1. The rex1-1 allele resulted in 3'-extended 5S rRNA. Polysome analysis revealed that rex1-1 and rrs1-5 caused a synergistic defect in the assembly of 60S ribosomal subunits. In vivo and in vitro binding assays indicate that Rrs1p interacts with the ribosomal protein L5-5S rRNA complex. The rrs1-5 mutation weakens the interaction between Rrs1p with both L5 and L11. These data suggest that the assembly of L5-5S rRNA on 60S ribosomal subunits coordinates with assembly of L11 via Rrs1p.
Nariai
Masanobu
M
Department of Bioresource Science and Technology, Graduate School of Biosphere Science, Hiroshima University Kagamiyama, Higashi-Hiroshima 739-8528, Japan.
Tanaka
Tomohisa
T
Okada
Takafumi
T
Shirai
Chiharu
C
Horigome
Chihiro
C
Mizuta
Keiko
K
D048708:Cell Growth Processes; D005800:Genes, Fungal; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D026721:RNA 3' End Processing; D012341:RNA, Ribosomal, 5S / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D009687:Nuclear Proteins; D012341:RNA, Ribosomal, 5S; C485770:RRS1 protein, S cerevisiae; D012269:Ribosomal Proteins; C027624:ribosomal protein L11; C029742:ribosomal protein L5