[Effect of yisui shengxue granule on gene expression in children with beta-thalassemia].
OBJECTIVE
To investigate the mechanism of yisui shengxue granule (YSG) in children with betathalassemia.


METHODS
The changes of hemoglobin (Hb), red blood cells (RBC), reticulocyte (Ret) and fetal hemoglobin (HbF) of 20 children with beta-thalassemia were measured before and after treatment. The effect of YSG on gene expression in myelo-karyocytes in 3 selected children treated effectively by YSG was determined by using DDRT-PCR.


RESULTS
The hematologic parameters were significantly enhanced and the ferritin gene expression declined after treatment respectively, as compared with those before treatment, the difference were significant (P < 0.01 or P < 0.05).


CONCLUSIONS
YSG could improve the clinical symptoms of children with beta-thalassemia. One of its therapeutic mechanisms may be its action in decreasing ferritin gene expression so as to effectively relieve the accumulation of iron in body.
Chai
Li-min
LM
Department of Molecular Biology, Guang' anmen Hospital, China Academy of Traditional Chinese Medicne, Beijing
Wu
Zhi-kui
ZK
Zhang
Xin-hua
XH
Cai
Huiguo
H
D001483:Base Sequence; D002648:Child; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D005293:Ferritins / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D008517:Phytotherapy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D017086:beta-Thalassemia / Q000188:drug therapy* / Q000235:genetics
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D005293:Ferritins