title: Differential identification of mycoplasma pulmonis and M. arthritidis using PCR-based RFLP.
abstract: Mycoplasma pulmonis and Mycoplasma arthritidis were differentially identified using PCR-restriction fragment length polymorphism (RFLP). A genus-specific sequence of mycoplasma was amplified by PCR and the PCR products were digested with the restriction enzyme SmaI. Each PCR product from the four isolates of M. pulmonis was digested with SmaI into two fragments; however, there was no digestion in the PCR product from M. arthritidis. This method might be useful to differentiate infection of M. pulmonis from that of M. arthritidis.
authors.affiliation: Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
authors.forename: Dong-Jae
authors.initials: DJ
authors.lastname: Kim
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Park
authors.forename: Seung-Hyeok
authors.initials: SH
authors.lastname: Seok
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Cho
authors.forename: Min-Won
authors.initials: MW
authors.lastname: Baek
authors.forename: Hui-Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Ki-Hwa
authors.initials: KH
authors.lastname: Yang
authors.forename: Dong Deuk
authors.initials: DD
authors.lastname: Jang
authors.forename: Beom-Seok
authors.initials: BS
authors.lastname: Han
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D001170:Arthritis, Infectious / Q000175:diagnosis / Q000662:veterinary*; D004269:DNA, Bacterial / Q000032:analysis; D003937:Diagnosis, Differential; D051379:Mice; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D045645:Mycoplasma arthritidis / Q000235:genetics / Q000302:isolation & purification*; D045803:Mycoplasma pulmonis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D012376:Rodent Diseases / Q000175:diagnosis*
chemical_list: D004269:DNA, Bacterial