title: Molecular taxonomic study of Chagas disease vectors from the phyllosoma, lecticularia, and rubrofasciata complexes.
abstract: The Triatiominae (Hemiptera: Reduviidae) are hematophagous hemipters of importance because they transmit Trypanosoma cruzi, the causal agent of Chagas disease. The aim of this study was to define the possible relationships between species of the Phyllosoma complex (Triatoma mazzottii, Triatoma pallidipennis, and Triatoma longipennis) and species of other complexes present in Mexico that have not been previously analyzed (Triatoma lecticularia and Triatoma rubida). In addition, it was determined the inclusion of Triatoma bassolsae in the Phyllosoma complex by using 10 isoenzymatic systems (corresponding to the 14 loci). Results of isoenzymatic study show that between the species of the Phyllosoma complex including Triatoma bassolsae, the polymorphism of the analyzed enzymes ranges from 14% to 50% (P < or = 0.95) and the species from external complexes showed polymorphism values of 43% (Triatoma lecticularia), 43% (Triatoma rubida), and 36% (Triatoma infestans). The genetic tree shows a clear difference between species of the Phyllosoma complex and the other complexes.
authors.affiliation: Department of Immunology, Biomedical Research Institute, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Martínez
authors.forename: Ricardo
authors.initials: R
authors.lastname: Alejandre-Aguilar
authors.forename: Yolanda Hortelano
authors.initials: YH
authors.lastname: Moncada
authors.forename: Bertha
authors.initials: B
authors.lastname: Espinoza
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000635:transmission*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D007303:Insect Vectors / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D008800:Mexico; D010802:Phylogeny; D011110:Polymorphism, Genetic; D013045:Species Specificity; D014224:Triatoma / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D014349:Trypanosoma cruzi
chemical_list: D007527:Isoenzymes