[Isolation and identification of taxane from Taxus chinensis cell cultures].
OBJECTIVE
To study taxanes of the cell cultures of Taxus chinensis.


METHODS
The cell cultures were extracted with 95% alcohol, distributed by different solvents, isolated via column chromatogaphy on silica gel by eluting with various proportions of petroleum ether-ethyl acetate and purified by crystallization with ethanol.


RESULTS
A taxane was isolated from the cell cultures of Taxus chinensis and its structure was identified as 2alpha,5alpha, 10beta, 14beta-tetraacetoxy-taxa-4 (20), 11-diene (Taxuyunnanine C) on the base of spectrum data.


CONCLUSIONS
The compound was isolated from the cell cultures of Taxus chinensis for the first time.
Zhou
Zhongqiang
Z
College of Chemistry and Life Sciences, South-Central University for Nationalities, Wuhan.
Mei
Xingguo
X
D002478:Cells, Cultured; D002681:China; D003470:Culture Media; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D043823:Taxoids / Q000737:chemistry / Q000302:isolation & purification*; D020946:Taxus / Q000737:chemistry* / Q000166:cytology
D003470:Culture Media; D043823:Taxoids