Flow-injection determination of streptomycin residues in milk using the luminol-periodate-Mn2+ chemiluminescence system.
A novel flow-injection method with chemiluminescence detection was developed for the determination of streptomycin residues in milk, based on the enhancement by streptomycin of the luminol-potassium periodate-Mn(2+) chemiluminescence system. The assay allowed analysis of streptomycin residues in whole milk samples ( approximately 3.5% fat) after sample work-up procedures. The limit of detection was 5.16 x 10(-9) mol/L for milk, and was far below the regulations of maximum residue limits (MRLs). The relative standard deviation (RSD) for 8.66 x 10(-8) mol/L streptomycin was 1.94% (n = 15) in milk. The results obtained for the assay of streptomycin residues in milk was comparable with those obtained by the official methods (ELISA) and demonstrated good accuracy and precision. The possible mechanism is briefly discussed.
Wan
Guo-Hui
GH
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
Cui
Hua
H
Zheng
Hai-Song
HS
Pang
Yong-Qiang
YQ
Liu
Li-Juan
LJ
Yu
Xiao-Feng
XF
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D017022:Flow Injection Analysis / Q000379:methods*; D007700:Kinetics; D008165:Luminol / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D010504:Periodic Acid / Q000737:chemistry*; D013307:Streptomycin / Q000032:analysis*
D000900:Anti-Bacterial Agents; D010504:Periodic Acid; D008345:Manganese; D008165:Luminol; D013307:Streptomycin