title: Cell cycle, DNA replication, repair, and recombination in the dimorphic human pathogenic fungus Paracoccidioides brasiliensis.
abstract: DNA replication, together with repair mechanisms and cell cycle control, are the most important cellular processes necessary to maintain correct transfer of genetic information to the progeny. These processes are well conserved throughout the Eukarya, and the genes that are involved provide essential information for understanding the life cycle of an organism. We used computational tools for data mining of genes involved in these processes in the pathogenic fungus Paracoccidiodes brasiliensis. Data derived from transcriptome analysis revealed that the cell cycle of this fungus, as well as DNA replication and repair, and the recombination machineries, are highly similar to those of the yeast Saccharomyces cerevisiae. Among orthologs detected in both species, there are genes related to cytoskeleton structure and assembly, chromosome segregation, and cell cycle control genes. We identified at least one representative gene from each step of the initiation of DNA replication. Major players in the process of DNA damage and repair were also identified.
authors.affiliation: Laboratório de Biologia Molecular, Departamento de Biologia Celular, Universidade de Brasília, 70910-900 Brasília, DF, Brazil.
authors.forename: Viviane Castelo Branco
authors.initials: VC
authors.lastname: Reis
authors.forename: Fernando Araripe Gonçalves
authors.initials: FA
authors.lastname: Torres
authors.forename: Marcio José
authors.initials: MJ
authors.lastname: Poças-Fonseca
authors.forename: Marlene Teixeira
authors.initials: MT
authors.lastname: De-Souza
authors.forename: Diorge Paulo de
authors.initials: DP
authors.lastname: Souza
authors.forename: João Ricardo Moreira
authors.initials: JR
authors.lastname: Almeida
authors.forename: Camila
authors.initials: C
authors.lastname: Marinho-Silva
authors.forename: Nádia Skorupa
authors.initials: NS
authors.lastname: Parachin
authors.forename: Alessandra da Silva
authors.initials: Ada S
authors.lastname: Dantas
authors.forename: Thiago Machado
authors.initials: TM
authors.lastname: Mello-de-Sousa
authors.forename: Lídia Maria Pepe de
authors.initials: LM
authors.lastname: Moraes
mesh_terms: D002453:Cell Cycle / Q000235:genetics* / Q000502:physiology; D004260:DNA Repair / Q000235:genetics* / Q000502:physiology; D004261:DNA Replication / Q000235:genetics* / Q000502:physiology; D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D006801:Humans; D009154:Mutation / Q000235:genetics; D010228:Paracoccidioides / Q000166:cytology / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D004271:DNA, Fungal