title: Combinatorial synthesis of MUC1 glycopeptides: polymer blotting facilitates chemical and enzymatic synthesis of highly complicated mucin glycopeptides.
abstract: The chemoselective polymer blotting method allows for rapid and efficient synthesis of glycopeptides based on a "catch and release" strategy between solid-phase and water-soluble polymer supports. We have developed a heterobifunctional linker sensitive to glutamic acid specific protease (BLase). The general procedure consists of five steps, namely (i) the solid-phase synthesis of glycopeptide containing BLase sensitive linker, (ii) subsequent deprotections and the release of the glycopeptide from the resin, (iii) chemoselective blotting of the glycopeptide intermediates in the presence of water-soluble polymers with oxylamino functional groups, (iv) sugar elongations using glycosyltransferases, and (v) the release of target glycopeptides from the polymer platform by selective BLase promoted hydrolysis. The combined use of the solid-phase chemical syntheses of peptides and the enzymatic syntheses of carbohydrates on water-soluble polymers would greatly contribute to the production of complicated glycopeptide libraries, thereby enhancing applicative research. We report here a high-throughput synthetic system for the various types of MUC1 glycopeptides exhibiting a variety of sugar moieties. It is our belief that this concept will become part of the entrenched repertoire for the synthesis of biologically important glycopeptides on the basis of glycosyltransferase reactions in automated and combinatorial syntheses.
authors.affiliation: Glycochemosynthesis Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo 062-8517, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Fumoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hinou
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohta
authors.forename: Takaomi
authors.initials: T
authors.lastname: Ito
authors.forename: Kuriko
authors.initials: K
authors.lastname: Yamada
authors.forename: Akio
authors.initials: A
authors.lastname: Takimoto
authors.forename: Hirosato
authors.initials: H
authors.lastname: Kondo
authors.forename: Hiroki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Inazu
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nakahara
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
mesh_terms: D002240:Carbohydrate Sequence; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012697:Serine Endopeptidases / Q000737:chemistry*
chemical_list: D006020:Glycopeptides; D009077:Mucins; D011108:Polymers; D012697:Serine Endopeptidases