title: Isolation and characterization of a white rot fungus Bjerkandera sp. strain capable of oxidizing phenanthrene.
abstract: Strain BOL13 was selected from 18 fungal strains isolated from an oil-spill contaminated site in Oruro, Bolivia. It was identified as a basidiomycete with high homology to Bjerkandera. The fungus degraded 100 mg phenanthrene l(-1) at 0.17 mg l(-1) d(-1) at 30 degrees C at pH 7. During phenanthrene degradation, a maximum manganese peroxidase activity of 100-120 U l(-1) was measured after 10 days of incubation. The ability of Bjerkandera sp. to produce lignin-modifying enzymes and to oxidize phenanthrene under various pH and temperature conditions was confirmed.
authors.affiliation: Biotechnology Department, Center for Chemistry and Chemical Engineering, Lund University, Sweden.
authors.forename: Enrique
authors.initials: E
authors.lastname: Terrazas-Siles
authors.forename: Teresa
authors.initials: T
authors.lastname: Alvarez
authors.forename: Benoit
authors.initials: B
authors.lastname: Guieysse
authors.forename: Bo
authors.initials: B
authors.lastname: Mattiasson
mesh_terms: D001487:Basidiomycota / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism; D010616:Phenanthrenes / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D010616:Phenanthrenes; C031181:phenanthrene; D008031:Lignin; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; D005947:Glucose