Partial nucleotide sequences and expression patterns of frog (Rana pipiens) ephrin-A2 and ephrin-A5 mRNA.
We have generated 362 bp and 547 bp partial sequences for Rana pipiens ephrin-A2 and ephrin-A5 mRNA, respectively. Translation homologies for the comparable segments of cDNA of chicken, mouse and human are 90.8, 86.9 and 84.4% for the ephrin-A2 sequence and 85.7, 85.0 and 85.0% for the ephrin-A5 sequence. Digoxigenin-labeled riboprobes were prepared and applied by means of in situ hybridization to whole-mounts of the brains of mature adults and expression patterns in tadpoles were also explored. The RNA probes revealed similar posterior (high) to anterior (low) expression gradients in the adult tectum, demonstrating that both ephrin-As are expressed in the adult Ranid frog tectum. Only the ephrin-A2 probe was tested on tadpole brain, yielding an appropriately graded expression pattern similar to the adult.
Yagita
Yoshiki
Y
Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Suita City, Osaka 565-0871, Japan.
Barjis
Isaac
I
Hecht
Michael
M
Bach
Helene
H
Feldheim
David A
DA
Scalia
Frank
F
D000818:Animals; D002645:Chickens / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D036383:Ephrin-A2 / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D036386:Ephrin-A5 / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D019143:Evolution, Molecular; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D006801:Humans / Q000235:genetics; D007814:Larva / Q000235:genetics / Q000378:metabolism; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D011894:Rana pipiens / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013477:Superior Colliculi / Q000378:metabolism
D018076:DNA, Complementary; D036383:Ephrin-A2; D036386:Ephrin-A5; D009711:Nucleotides; D012333:RNA, Messenger