Characterisation of long term cat placental explant cultures: uptake of taurine by system beta.
Dietary taurine is essential for cats and deficiency during pregnancy may lead to abortion, growth restriction or impaired neurological function of kittens. We previously described Na(+)- and Cl(-)-dependent taurine transport by system beta in fragments of freshly isolated cat placenta [Champion EE, Bailey SJ, Glazier JD, Jones CJP, Mann SJ, Rawlings JM, et al. Taurine uptake into cat placental tissue fragments. Placenta 2001;22:A.42]. Here we evaluate long term culture of cat placental explants as a model for the future study of chronic nutrient regulation of amino acid transport in this species. The cat placental explants displayed (i) Na(+)-dependent [(3)H]taurine uptake and (ii) taurine transporter protein on day 7 of culture, as observed in fresh cat placental fragments. The explants had preserved the ability to secrete PGF(2alpha) hormone until day 11 of culture and remained morphologically largely intact until day 7 of culture. This model of placental explant culture will provide an important in vitro method for the study of chronic regulation of amino acid transport in the cat.
Champion
E E
EE
Academic Unit of Child Health, University of Manchester, St. Mary's Hospital, Manchester, UK.
Mann
S J
SJ
Glazier
J D
JD
Jones
C J P
CJ
Rawlings
J M
JM
Sibley
C P
CP
Greenwood
S L
SL
D026905:Amino Acid Transport Systems / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002415:Cats; D015237:Dinoprost / Q000378:metabolism; D005260:Female; D023421:Models, Animal; D009924:Organ Culture Techniques; D010920:Placenta / Q000033:anatomy & histology / Q000378:metabolism*; D013654:Taurine / Q000378:metabolism*
D026905:Amino Acid Transport Systems; D015415:Biomarkers; D013654:Taurine; D015237:Dinoprost