title: The yeaS (leuE) gene of Escherichia coli encodes an exporter of leucine, and the Lrp protein regulates its expression.
abstract: Overexpression of the yeaS gene encoding a protein belonging to the RhtB transporter family conferred upon cells resistance to glycyl-l-leucine, leucine analogues, several amino acids and their analogues. yeaS overexpression promoted leucine and, to a lesser extent, methionine and histidine accumulation by the respective producing strains. Our results indicate that yeaS encodes an exporter of leucine and some other structurally unrelated amino acids. The expression of yeaS (renamed leuE for "leucine export") was induced by leucine, l-alpha-amino-n-butyric acid and, to a lesser extent, by several other amino acids. The global regulator Lrp mediated this induction.
authors.affiliation: Ajinomoto-Genetika Research Institute, Laboratory N2, 1-st Dorozhny Proezd, b.1, Moscow 117545, Russia.
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Kutukova
authors.forename: Vitaliy A
authors.initials: VA
authors.lastname: Livshits
authors.forename: Irina P
authors.initials: IP
authors.lastname: Altman
authors.forename: Leonid R
authors.initials: LR
authors.lastname: Ptitsyn
authors.forename: Michael H
authors.initials: MH
authors.lastname: Zyiatdinov
authors.forename: Irina L
authors.initials: IL
authors.lastname: Tokmakova
authors.forename: Natalia P
authors.initials: NP
authors.lastname: Zakataeva
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006639:Histidine / Q000378:metabolism; D007930:Leucine / Q000737:chemistry / Q000378:metabolism*; D051756:Leucine-Responsive Regulatory Protein; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D014157:Transcription Factors; D024363:Transcription Initiation Site
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C504239:LeuE protein, E coli; C497278:Lrp protein, E coli; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C119928:RhtB protein, E coli; D014157:Transcription Factors; D051756:Leucine-Responsive Regulatory Protein; D006639:Histidine; D008715:Methionine; D007930:Leucine