The role of protein kinase activity expressed by the UL13 gene of herpes simplex virus 1: the activity is not essential for optimal expression of UL41 and ICP0.
Herpes simplex virus 1 (HSV-1) UL13 is a viral protein kinase that is packaged into virions and regulates optimal expression of ICP0 and a subset of late (gamma) proteins, including UL41 in infected cells. In the present study, we investigated the role(s) of the protein kinase activity of UL13 in viral replication using a recombinant virus expressing enzymatically inactive UL13 after an amino acid substitution in the invariant lysine of UL13. The recombinant virus carrying this mutation formed smaller plaques yielded 10-fold less progeny than wild-type virus but could not be differentiated from wild-type virus with respect to accumulation of UL41 and ICP0 in infected cells. These results indicate that the protein kinase activity of UL13 plays a role in viral replication in cell culture, but the activity is not essential for the optimal expression of UL41 and ICP0.
Tanaka
Michiko
M
Department of Pathology, National Institute of Infectious Disease, Shinjuku-ku, Tokyo 162-8640, Japan.
Nishiyama
Yukihiro
Y
Sata
Tetsutaro
T
Kawaguchi
Yasushi
Y
D019943:Amino Acid Substitution; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D015967:Gene Expression Regulation, Viral; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000502:physiology*; D017874:Immediate-Early Proteins / Q000096:biosynthesis* / Q000235:genetics; D015151:Immunoblotting; D020125:Mutation, Missense; D011494:Protein Kinases / Q000235:genetics / Q000502:physiology*; D011817:Rabbits; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis* / Q000235:genetics; D014709:Vero Cells; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics; D014779:Virus Replication
D017874:Immediate-Early Proteins; C081688:Ul41 protein, Human herpesvirus 1; D014764:Viral Proteins; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1; D011494:Protein Kinases; C082380:UL13 protein, Simplexvirus