Immune response to Saccharomyces cerevisiae mannan conjugate in mice.
Mannan, the surface polysaccharide antigen of Saccharomyces cerevisiae was partially oxidized and conjugated to a protein carrier. Prepared conjugate was immunogenic in mice and re-injection elicited significant increase of anti-S. cerevisiae specific IgG and IgM serum antibodies. There was somewhat less increase in IgM serum antibodies. Serum distribution of IgG subclasses, especially IgG(2(a+b)):IgG(1) ratio throughout the immunization demonstrated effective Th1 predominance of immune response. Newly synthesized S. cerevisiae mannan conjugate could be considered as a perspective vaccine candidate for preventive immunomodulation treatment.
Paulovicová
Ema
E
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
Bystrický
Slavomír
S
Masárová
Jana
J
Machová
Eva
E
Mislovicová
Danica
D
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood / Q000276:immunology*; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology*; D008351:Mannans / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008813:Mice, Inbred ICR; D012441:Saccharomyces cerevisiae / Q000276:immunology* / Q000378:metabolism; D018074:Vaccines, Conjugate / Q000276:immunology*
D000908:Antibodies, Fungal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008351:Mannans; D018074:Vaccines, Conjugate