[Hybridization and their application in the DNA array technology].
Hybridization and their application in the DNA array technology. DNA hybridization arrays measure simultaneously the expression of several genes. First, a known DNA sequence (probe) is fixed on a firm basis. Then the complementer sequence (target sequence) is linked to it during the hybridization process. The target sequence extracted from biological samples is fluorescently, enzimatically or radioactively labeled before detection. Higher expression results in higher signal in the detection system. Unlabeled DNA strands can also be detected, as the electronic and optical characteristics of the DNA is altered after complementer hybridization. In this review we summarize the basics of hybridisation and its newest application area in the DNA array systems.
Gyorffy
András
A
Semmelweis Egyetem, Altalános Orvostudomanyi Kar, II. Belgyógyászati Klinika, MTA-Semmelweis Egyetem Gasztroenterolégiai es Endokrinológiai Kutató Csoport, Budapest. foeszi@yahoo.com
Gyorffy
Balázs
B
Molnár
Béla
B
Tulassay
Zsolt
Z
D000818:Animals; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis