[Preparation of alpha-toxin's protective antigen of clostridium perfringens type A and research for its primary immunological protective function].
Induced by 42 degrees C, the recombinant engineering bacterial pBV/cpa408 was highly expressed. After having been pelleted by 80% (NH4)2 SO4 and dialysised, the expressed protein was isolated and purified by the gel filtration choromatography. Then according to an amount of 1.0 mg/kg, the Kunming Mice (body weighted 18g) were immuned with the purified protein by intraperitoneal inoculation. One week after the first enhanced immunization, the Kunming Mice were attacked with an amount of 1.0MLD alpha-toxin, in which the eight mice immuned all survive and the control group all died. During the period of immunization, the titre of the mouse's serum antibody was measured by ELISA. One week after the first immunization, the titre of the mice's serum antibody was 1:800, but that of one week after the first enhanced immunization reached to 1:6400.
Lin
Ming-Hui
MH
Laboratory Department of Kunming General Hospital, Kunming 650032, China.
Yin
Jun
J
Xing
Hong-Guang
HG
Hou
Xiao-Jun
XJ
Wang
Hui
H
Song
Wei
W
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D001427:Bacterial Toxins / Q000276:immunology*; D002135:Calcium-Binding Proteins / Q000276:immunology*; D005260:Female; D007114:Immunization; D008297:Male; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D010738:Type C Phospholipases / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D002135:Calcium-Binding Proteins; D011994:Recombinant Proteins; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens