Precise proteomic identification using mass spectrometry coupled with stable isotope labeling.
Stable isotope labeling (SIL) can assist mass spectrometry to improve its specificity and throughput in protein identification, de novo sequencing, and characterization of post translational modifications. This Education article summarizes the unique characteristics of stable isotope-assisted mass spectrometry for accurate protein identification without requirements for ultrahigh mass accuracy. Some applications are discussed here to demonstrate the general experimental procedures, data interpretation, and the improvements in accuracy and throughput compared to the use of mass spectrometry alone.
Gu
Sheng
S
MS M888, Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Chen
Xian
X
D000818:Animals; D006801:Humans; D007553:Isotope Labeling; D013058:Mass Spectrometry; D040901:Proteomics / Q000379:methods*; D020539:Sequence Analysis, Protein