Berberine inhibits arylamine N-acetyltransferase activity and gene expression in Salmonella typhi.
The effects of berberine on growth, arylamine N-acetyltransferase (NAT) activity, and gene expression in Salmonella Typhi (Typhi) were described. The growth inhibition of Typhi was determined by measuring absorbance by optical density (OD at 650 nm). The NAT activity was determined by measuring the levels of 2-aminofluorene (AF) and N-acetyl-2-aminofluorene (AAF) by high-performance liquid chromatography. The results demonstrated that 24-h berberine treatment decreased bacteria growth and amounts of AAF in Typhi. Western blotting and flow cytometry were used for examining the levels of NAT after bacteria were cotreated with or without various concentrations of berberine, and results indicated that berberine decreased the levels of NAT in Typhi. Polymerase chain reaction was used for examining the gene expression of NAT (mRNA NAT), and results indicated that berberine affects mRNA NAT1 expression in Typhi.
Wu
Lii-Tzu
LT
Department of Microbiology, China Medical University, Taichung, Taiwan.
Tsou
Mei-Fen
MF
Ho
Chin-Chin
CC
Chuang
Jing-Yuan
JY
Kuo
Hsiu-Maan
HM
Chung
Jing-Gung
JG
D001191:Arylamine N-Acetyltransferase / Q000378:metabolism*; D001599:Berberine / Q000494:pharmacology*; D028142:Berberis; D015536:Down-Regulation; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D012485:Salmonella typhi / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D001599:Berberine; D001191:Arylamine N-Acetyltransferase