A universal HPLC method for the determination of phenolic acids in compound herbal medicines.
A universal method to separate and quantify 13 phenolic acids (gallic acid, chlorogenic acid, gentsic acid, vanillic acid, caffeic acid, syringic acid, sinapic acid, p-coumaric acid, ferulic acid, anisic acid, rosmarinic acid, salicylic acid, and cinnamic acid) in some compound herbal medicines was established by liquid chromatographic (HPLC). On an Agela XBP-C18 (5 microm, 4.6 mm x 150 mm) column, a multistep binary gradient elution program and a simplified sample pretreatment approach were used in the experiment. For all of the phenolic acids, detection limits ranged around 0.01 mg/L. Linear ranges of higher than 2 orders of magnitude were obtained with a correlation coefficient of 0.9991 to 1. Repeatability was 0.39-2.24% (relative standard deviation, RSD) for intraday, 1.17-3.96% (RSD) for interday, and 0.14-5.33% (RSD) for drug sample analysis. Recovery, tested by a standard addition method, ranged from 83.3% to 104.9% for various trace phenolic acids.
Wen
Dawei
D
Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
Li
Chenchen
C
Di
Hao
H
Liao
Yiping
Y
Liu
Huwei
H
D000146:Acids, Carbocyclic / Q000032:analysis*; D002109:Caffeic Acids; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D062385:Hydroxybenzoates / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results
D000146:Acids, Carbocyclic; D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; C017616:phenolic acid; C040048:caffeic acid