title: Expression, purification and partial characterization of the Krüppel-associated box (KRAB) from the human ZNF2 protein.
abstract: The Krüppel-associated box (KRAB) domain is a potent transcription repression bipartite domain, shared by over 400 zinc finger proteins in humans, involved in the regulation of many functions. KRAB domains are both physically and functionally bipartite (A and B modules). The lone KRAB-A and composite KRAB-AB domains from the human ZNF2 protein were over-expressed as recombinant proteins in E. coli, isolated and purified to homogeneity to investigate their structure to function relationship.
authors.affiliation: Istituto di Genetica e Biofisica A. Buzzati Traverso, Consiglio Nazionale delle Ricerche, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Rivieccio
authors.forename: Riccardo
authors.initials: R
authors.lastname: Mannini
authors.forename: Luigi
authors.initials: L
authors.lastname: Concilio
authors.forename: Sabato
authors.initials: S
authors.lastname: D'Auria
authors.forename: Carlo
authors.initials: C
authors.lastname: Pedone
authors.forename: Giovanna
authors.initials: G
authors.lastname: Grimaldi
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D011994:Recombinant Proteins; C118913:ZNF2 protein, human