High pressure near infrared study of the mutated light-harvesting complex LH2.
The pressure sensitivities of the near infrared spectra of the light-harvesting (LH2) complex and a mutant complex with a simplified BChl-B850 binding pocket were compared. In the mutant an abrupt change in the spectral properties occurred at 250 MPa, which was not observed with the native sample. Increased disorder due to collapse of the chromophore pocket is suggested.
Braun
P
P
Department of Biology, University of Munich, Munich, Germany.
Gebhardt
R
R
Kwa
L
L
Doster
W
W
D004926:Escherichia coli / Q000235:genetics*; D006874:Hydrostatic Pressure; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics*; D012242:Rhodobacter sphaeroides / Q000235:genetics*; D019265:Spectroscopy, Near-Infrared
D045342:Light-Harvesting Protein Complexes