title: Atelocollagen for culture of human nucleus pulposus cells forming nucleus pulposus-like tissue in vitro: influence on the proliferation and proteoglycan production of HNPSV-1 cells.
abstract: Nucleus pulposus (NP) is responsible for maintaining function and structure of the disc. Scaffolds to culture disc cells three-dimensionally are emphasized in recent reports on development of a new method for treating disc degeneration using cell transplantation and tissue engineering. Among artificial scaffolds and cell carrying materials, Atelocollagen is a collagen gel that has an advantage in safety issues over others. However, to date there has been no study that investigated culture of human nucleus pulposus cells in Atelocollagen. To investigate whether Atelocollagen could be used as a culture scaffold and if it has any effect on cell proliferation and proteoglycan (PG) production, as well as to find the optimal commercially available Atelocollagen for NP cell transplantation and tissue engineering, we cultured human NP cell line HNPSV-1, in three different Atelocollagen and compared with alginate. Furthermore, NP-like tissues were generated using these cells and different Atellocollagen solutions. Results showed that both DNA synthesis and content is significantly greater when cultured in Atelocollagen than in alginate. On the other hand, proteoglycan synthesis and accumulation was significantly greater in alginate compared with the 0.3% Atelocollagen scaffolds; with 3% Atelocollagen, however, results were similar. NP-like tissue generated by Atelocollagen showed good water and proteoglycan preservation. The current study demonstrates that the use of Atelocollagen as an in vitro culture scaffold for three-dimensional culture of human NP cell lines is indeed feasible and moreover, Atelocollagen possesses the potential to become a candidate scaffold for cell transplantation or tissue engineering for the treatment of intervertebral disc degeneration.
authors.affiliation: Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan. daisakai@is.icc.u-tokai.ac.jp
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sakai
authors.forename: Joji
authors.initials: J
authors.lastname: Mochida
authors.forename: Toru
authors.initials: T
authors.lastname: Iwashina
authors.forename: Takuya
authors.initials: T
authors.lastname: Watanabe
authors.forename: Kaori
authors.initials: K
authors.lastname: Suyama
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ando
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Hotta
mesh_terms: D000328:Adult; D000464:Alginates / Q000494:pharmacology; D001672:Biocompatible Materials / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology*; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D005260:Female; D020723:Glucuronic Acid / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000032:analysis / Q000096:biosynthesis; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D007403:Intervertebral Disc / Q000166:cytology*; D009940:Organoids / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011509:Proteoglycans / Q000096:biosynthesis*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006025:Glycosaminoglycans; D006603:Hexuronic Acids; D011509:Proteoglycans; C059821:atelocollagen; C013786:A73025; D020723:Glucuronic Acid; D003094:Collagen; D004247:DNA