The CellCard System: a novel approach to assessing compound selectivity for lead prioritization of G protein-coupled receptors.
Advances in high throughput screening technologies have led to the identification of many small molecules, "hits", with activities toward the target of interest. And, as the screening technologies become faster and more robust, the rate at which the molecules are identified continues to increase. This evolution of high throughput screening technologies has generated a significant strain on the laboratories involved with the downstream profiling of these hits using cell-based assays. The CellCard System, by enabling multiple targets and/or cell lines to be assayed simultaneously within a single well, provides a platform on which selectivity screening can be quickly and robustly performed. Here we describe two case studies using the beta-lactamase and beta-galactosidase reporter gene systems to characterize G protein-coupled receptor agonist activity. Using these examples we demonstrate how the implementation of this technology enables assay miniaturization without micro-fluidic devices as well as how the inclusion of intra-well controls can provide a means of data quality assessment within each well.
Beske
Oren
O
Vitra Bioscience, Inc., 2450 Bayshore Parkway, Mountain View, CA 94043, USA. obeske@vitrabio.com
Goldbard
Simon
S
Turpin
Pierre
P
D000818:Animals; D001681:Biological Assay; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D017930:Genes, Reporter; D006801:Humans; D043562:Receptors, G-Protein-Coupled / Q000819:agonists / Q000187:drug effects* / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism; D001618:beta-Lactamases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D043562:Receptors, G-Protein-Coupled; D001616:beta-Galactosidase; D001618:beta-Lactamases