title: Characterization of a poplar NIMA-related kinase PNek1 and its potential role in meristematic activity.
abstract: Meristems are sites of undifferentiated cell division, which carry on developing into functional organs. Using the two-hybrid system with a poplar 14-3-3, we uncovered poplar NIMA-related kinase 1 (PNek1) as an interacting protein. PNek1 shows high homology to the mammalian NIMA-related kinases, which are thought to be involved in cell cycle progression. Using a synchronized poplar cell suspension, we observed an accumulation of PNek1 mRNA at the G1/S transition and throughout the G2-to-M progression. Moreover, PNek1-GFP fusion protein localized in the cytoplasm and in both the nuclear and nucleolar regions. Overexpression of PNek1-GFP in Arabidopsis caused morphological abnormalities in flower and siliques. Overall, these results suggest that PNek1 is involved in plant development.
authors.affiliation: Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 3800, Sainte-Foy, Que., Canada.
authors.forename: Monikca
authors.initials: M
authors.lastname: Cloutier
authors.forename: Frédéric
authors.initials: F
authors.lastname: Vigneault
authors.forename: Denis
authors.initials: D
authors.lastname: Lachance
authors.forename: Armand
authors.initials: A
authors.lastname: Séguin
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000033:anatomy & histology / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D018519:Meristem / Q000502:physiology*; D008969:Molecular Sequence Data; D000072380:NIMA-Related Kinase 1; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D032107:Populus / Q000033:anatomy & histology / Q000201:enzymology* / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques
chemical_list: D048948:14-3-3 Proteins; D018797:Cell Cycle Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C000606914:NEK1 protein, human; D000072380:NIMA-Related Kinase 1; D017346:Protein Serine-Threonine Kinases