title: Immune response to Saccharomyces cerevisiae mannan conjugate in mice.
abstract: Mannan, the surface polysaccharide antigen of Saccharomyces cerevisiae was partially oxidized and conjugated to a protein carrier. Prepared conjugate was immunogenic in mice and re-injection elicited significant increase of anti-S. cerevisiae specific IgG and IgM serum antibodies. There was somewhat less increase in IgM serum antibodies. Serum distribution of IgG subclasses, especially IgG(2(a+b)):IgG(1) ratio throughout the immunization demonstrated effective Th1 predominance of immune response. Newly synthesized S. cerevisiae mannan conjugate could be considered as a perspective vaccine candidate for preventive immunomodulation treatment.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
authors.forename: Ema
authors.initials: E
authors.lastname: Paulovicová
authors.forename: Slavomír
authors.initials: S
authors.lastname: Bystrický
authors.forename: Jana
authors.initials: J
authors.lastname: Masárová
authors.forename: Eva
authors.initials: E
authors.lastname: Machová
authors.forename: Danica
authors.initials: D
authors.lastname: Mislovicová
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood / Q000276:immunology*; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology*; D008351:Mannans / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008813:Mice, Inbred ICR; D012441:Saccharomyces cerevisiae / Q000276:immunology* / Q000378:metabolism; D018074:Vaccines, Conjugate / Q000276:immunology*
chemical_list: D000908:Antibodies, Fungal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008351:Mannans; D018074:Vaccines, Conjugate