title: Resistin SNP-420 determines its monocyte mRNA and serum levels inducing type 2 diabetes.
abstract: Resistin, secreted from adipocytes, causes insulin resistance in rodents. Its roles and main source in humans remain unknown. The G/G genotype of resistin single nucleotide polymorphism, SNP-420, induces type 2 diabetes mellitus (T2DM) by increasing promoter activity. We elucidated factors correlated with serum resistin and effects of SNP-420 on monocyte resistin mRNA. In 198 T2DM and 157 controls, fasting serum resistin was higher in T2DM. Multiple regression analysis revealed that SNP-420 genotype was the strongest determinant of serum resistin. In T2DM, 1-year duration of T2DM and 1% HbA1c was also correlated with 0.19 and 0.54 ng/ml serum resistin, respectively. Logistic regression analysis revealed that serum resistin was an independent factor for T2DM. In 23 healthy volunteers, monocyte resistin mRNA was positively correlated with its simultaneous serum levels and was higher in G/G genotype. Thus, SNP-420 determines monocyte mRNA and serum levels of resistin, which could induce T2DM.
authors.affiliation: Department of Laboratory Medicine, Ehime University School of Medicine, Shitsukawa, Toon, Ehime 791-0295, Japan. harosawa@m.ehime-u.ac.jp
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Osawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Onuma
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ochi
authors.forename: Akiko
authors.initials: A
authors.lastname: Murakami
authors.forename: Junko
authors.initials: J
authors.lastname: Yamauchi
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takasuka
authors.forename: Fumiko
authors.initials: F
authors.lastname: Tanabe
authors.forename: Ikki
authors.initials: I
authors.lastname: Shimizu
authors.forename: Kenichi
authors.initials: K
authors.lastname: Kato
authors.forename: Wataru
authors.initials: W
authors.lastname: Nishida
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yamada
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Tabara
authors.forename: Masaki
authors.initials: M
authors.lastname: Yasukawa
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Fujii
authors.forename: Jun
authors.initials: J
authors.lastname: Ohashi
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Miki
authors.forename: Hideichi
authors.initials: H
authors.lastname: Makino
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004247:DNA / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000235:genetics*; D005260:Female; D005838:Genotype; D006729:Hormones, Ectopic / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D012044:Regression Analysis; D052243:Resistin; D017422:Sequence Analysis, DNA
chemical_list: D006729:Hormones, Ectopic; C499624:RETN protein, human; D012333:RNA, Messenger; D052243:Resistin; D004247:DNA