title: Chromatin-related proteins in pluripotent mouse embryonic stem cells are downregulated after removal of leukemia inhibitory factor.
abstract: Embryonic stem (ES) cells have generated enormous interest due to their capacity to self-renew and the potential for growing many different cell types in vitro. Leukemia inhibitory factor (LIF), bone morphogenetic proteins, octamer-binding protein 3 or 4, and Nanog are important factors in the maintenance of pluripotency in mouse ES cells. However, the mechanisms by which these factors regulate the pluripotency remain poorly understood. To identify other proteins involved in this process, we did a proteomic analysis of mouse ES cells that were cultured in the presence or absence of LIF. More than 100 proteins were found to be involved specifically in either the differentiation process or the maintenance of undifferentiated state. Among these, chromatin-related proteins were identified as the major proteins in nuclear extracts of undifferentiated cells. Analysis with real-time RT-PCR revealed that enrichment of these proteins in pluripotent ES cells was regulated at the transcriptional levels. These results suggest that specific chromatin-related proteins may be involved in maintaining the unique properties of pluripotent ES cells.
authors.affiliation: The Institute for Enzyme Research, University of Tokushima, 3-18-15, Kuramoto, Tokushima 770-8503, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kurisaki
authors.forename: Tatsuo S
authors.initials: TS
authors.lastname: Hamazaki
authors.forename: Koji
authors.initials: K
authors.lastname: Okabayashi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Iida
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nishine
authors.forename: Ritsu
authors.initials: R
authors.lastname: Chonan
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kido
authors.forename: Susumu
authors.initials: S
authors.lastname: Tsunasawa
authors.forename: Osamu
authors.initials: O
authors.lastname: Nishimura
authors.forename: Makoto
authors.initials: M
authors.lastname: Asashima
authors.forename: Hiromu
authors.initials: H
authors.lastname: Sugino
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000502:physiology*; D015536:Down-Regulation; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002843:Chromatin; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse