title: [Method of polymerase chain reaction in toxoplasmosis diagnosis].
abstract: Since introduction of polymerase chain reaction for the detection of DNA Toxoplasma gondii 398 biological samples from 301 patients were examined from 2000 to 2003. Positive finding of toxoplasmosis DNA we noted in 23 cases. Polymerase chain reaction enables exact and fast diagnosis of the actual parasitemia Toxoplasma gondii, which is important especially in the pregnant patients, new-born with suspicion on congenital toxoplasmosis and in the patients with immunosupression.
authors.affiliation: Fakultní nemocnice v Hradci Králové, Ustav klinické mikrobiologie. cermakovaz@fnhk.cz
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Cermáková
authors.forename: Lenka
authors.initials: L
authors.lastname: Plísková
authors.forename: Petr
authors.initials: P
authors.lastname: Prásil
authors.forename: Olga
authors.initials: O
authors.lastname: Rysková
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis*; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D014122:Toxoplasma / Q000235:genetics; D014123:Toxoplasmosis / Q000175:diagnosis*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis; D014126:Toxoplasmosis, Ocular / Q000175:diagnosis
chemical_list: D016054:DNA, Protozoan