Excision of pyrimidine dimers by several UV-sensitive mutants of S. pombe.
Nine radiation-sensitive mutants of S. pombe showing a variety of phenotypic characteristics were analysed for their ability to excise pyrimidine dimers after ultraviolet irradiation. From earlier studies using indirect parameters, it was expected that some would be excision-deficient. Data reported here show that all the mutants tested, like wild type cells, were able to remove a high percentage of pyrimidine dimers during post-irradiation incubation in several different holding media, but not in saline or phosphate buffer. These mutants included strains showing increased, as well as others which showed decreased, levels of UV-induced mutation frequency relative to that of the wild type at the same total dose.
Birnboim
H C
HC
Biology and Health Physics Division, Atomic Energy of Canada Limited, Chalk River, Ontario, Canada.
Nasim
A
A
D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011740:Pyrimidine Dimers / Q000737:chemistry* / Q000302:isolation & purification; D012568:Schizosaccharomyces / Q000737:chemistry / Q000235:genetics* / Q000528:radiation effects*; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D011740:Pyrimidine Dimers