Effects of supercoiling on the sequence-specific photo-modification of DNA by a vanadium(V)-peroxo complex.
Sequence-specific photo-modification of DNA has been demonstrated, for the first time, in a vanadium(V)-peroxo complex, NH4[VO(O2)2(5,6-Me2phen)] (where 5,6-Me2phen = 5,6-dimethyl-1,10-phenanthroline). Using molecular cloning technique, a consensus sequence motif of 5'-G(A/G)TA(T/C)C was identified associated with the two specific photo-modification sites, 5'-ATC and 5'-TACC found on a plasmid DNA, pBluescript, by a modified Sanger sequencing technique. DNA supercoiling was shown to be a critical prerequisite for this observed sequence-specific photo-modification activity.
Kwong
Daniel W J
DW
Department of Chemistry, Hong Kong Baptist University, Hong Kong, PR China. dkwong@hkbu.edu.hk
Chan
O Y
OY
Shek
L K
LK
Wong
Ricky N S
RN
D001483:Base Sequence; D003001:Cloning, Molecular; D004278:DNA, Superhelical; D008969:Molecular Sequence Data; D009942:Organometallic Compounds / Q000737:chemistry*; D010545:Peroxides / Q000737:chemistry*; D010777:Photochemistry; D010957:Plasmids; D017968:Vanadium Compounds / Q000737:chemistry*
D004278:DNA, Superhelical; D009942:Organometallic Compounds; D010545:Peroxides; D017968:Vanadium Compounds; C505746:ammonium oxodiperoxo(5,6-dimethyl-1,10-phenanthroline)vanadate(III)