Development of a one-step strip test for the diagnosis of chicken infectious bursal disease.
A rapid diagnostic strip for chicken infectious bursal disease (IBD) was developed based on membrane chromatography using high-affinity monoclonal antibodies directed to chicken infectious bursal disease virus (IBDV). The diagnostic strip has high specificity for detection of chicken IBDV antigen and recognizes a variety of the virus isolates, including virulent and attenuated strains, with no cross-reactivity to other viruses, such as Newcastle disease virus, Marek's disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, and egg-drop-syndrome virus. The results showed that its specificity was highly consistent with the agar-gel precipitation test (AGP). The diagnostic strip detected as low as 800 median egg lethal dose (ELD50) viruses in the IBDV BC6/85-infected sample, which was comparable with AC-ELISA (400 ELD50) and 32 times more sensitive than the AGP test (2.56 x 10(4) ELD50). In experimental infection, IBDV was detected in the bursa as early as 36 hr postinfection with the diagnostic strip before the clinical signs and gross lesions appeared. It takes only 1-2 min to do a strip test to detect chicken IBDV antigen after the specimen is grounded in a whirl pack with finger massage.
Zhang
Gai-Ping
GP
Institute of Biotechnology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Li
Qing-Mei
QM
Yang
Yan-Yan
YY
Guo
Jun-Qing
JQ
Li
Xue-Wu
XW
Deng
Rui-Guang
RG
Xiao
Zhi-Jun
ZJ
Xing
Guang-Xu
GX
Yang
Ji-Fei
JF
Zhao
Dong
D
Cai
Shu-Jun
SJ
Zang
Wei-Min
WM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000302:isolation & purification*; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D002460:Cell Line; D002645:Chickens; D019937:Diagnostic Techniques and Procedures / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D007243:Infectious bursal disease virus / Q000276:immunology*; D051379:Mice; D011201:Poultry Diseases / Q000175:diagnosis*; D011817:Rabbits; D011934:Reagent Strips
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D011934:Reagent Strips