title: Applications of the double-barreled data in whole-genome shotgun sequence assembly and analysis.
abstract: Double-barreled (DB) data have been widely used for the assembly of large genomes. Based on the experience of building the whole-genome working draft of Oryza sativa L. ssp. Indica, we present here the prevailing and improved uses of DB data in the assembly procedure and report on novel applications during the following data-mining processes such as acquiring precise insert fragment information of each clone across the genome, and a new kind of low-cost whole-genome microarray. With the increasing number of organisms being sequenced, we believe that DB data will play an important role both in other assembly procedures and in future genomic studies.
authors.affiliation: College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Han
authors.forename: Peixiang
authors.initials: P
authors.lastname: Ni
authors.forename: Hong
authors.initials: H
authors.lastname: Lü
authors.forename: Jia
authors.initials: J
authors.lastname: Ye
authors.forename: Jianfei
authors.initials: J
authors.lastname: Hu
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Xiangang
authors.initials: X
authors.lastname: Huang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Cong
authors.forename: Guangyuan
authors.initials: G
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Gu
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Songgang
authors.initials: S
authors.lastname: Li
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D015723:Gene Library; D018745:Genome, Plant; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D018744:DNA, Plant