Separation and determination of clotrimazole, methylparaben and propylparaben in pharmaceutical preparation by micellar electrokinetic chromatography.
In this study, micellar electrokinetic chromatography (MEKC) method was developed for the determination of clotrimazole (CLO), methylparaben (MP) and propylparaben (PP) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (60 cm x 75 microm i.d.) at 25 kV with UV detection at 212 nm. Optimized background electrolyte (BGE) was 15 mM phosphate buffer (pH 7.2) containing 30 mM sodium dodecyl sulfate (SDS) as a surfactant. Rectilinear calibration ranges were 50-500 mg l(-1) for CLO, 10-100 mg l(-1) for MP and 2.5-25 mg l(-1) for PP. The total analysis time was < 12 min.
Hamoudová
Rafífa
R
Charles University, Faculty of Pharmacy, Department of Analytical Chemistry, Heyrovského 1203, CZ-500 05 Hradec Králové, Czech Republic.
Pospísilová
Marie
M
Kavalírová
Andrea
A
Solich
Petr
P
Sícha
Jan
J
D000891:Anti-Infective Agents, Local / Q000032:analysis / Q000737:chemistry; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003022:Clotrimazole / Q000032:analysis* / Q000737:chemistry; D004573:Electrolytes; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010226:Parabens / Q000032:analysis* / Q000737:chemistry; D010710:Phosphates / Q000032:analysis; D011310:Preservatives, Pharmaceutical / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013501:Surface-Active Agents / Q000032:analysis; D013696:Temperature; D013997:Time Factors
D000891:Anti-Infective Agents, Local; D004573:Electrolytes; D010226:Parabens; D010710:Phosphates; D011310:Preservatives, Pharmaceutical; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C015358:methylparaben; D003022:Clotrimazole; C006068:propylparaben