Place of pattern in proteomic biomarker discovery.
The role of pattern in biomarker discovery and clinical diagnosis is examined in its historical context. The use of MS-derived pattern is treated as a logical extension of prior applications of non-MS-derived pattern. Criticisms pertaining to specific technology platforms and analytic methodologies are considered separately from the larger issues of pattern utility and deployment in biomarker discovery. We present a hybrid strategy that marries the desirable attributes of high-information content MS pattern with the capability to obtain identity, and explore the key steps in establishing a data analysis pipeline for pattern-based biomarker discovery.
Gillette
Michael A
MA
The Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, MA 02141, USA. gillette@broad.mit.edu
Mani
D R
DR
Carr
Steven A
SA
D015415:Biomarkers / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry; D025202:Molecular Diagnostic Techniques; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D015203:Reproducibility of Results
D015415:Biomarkers; D020543:Proteome