[Role of cyclooxygenase-2 in inhibition of human gastric adenocarcinoma cell line SGC-7901 by c9, t11-conjugated linoleic acid].
OBJECTIVE
To study the effects of c9, t11-conjugated linoleic acid (c9, t11-CLA) on the critical enzyme (COX-2) and its product - prostaglandin E2 (PGE2) of linoleic acid metabolism path in human gastric adenocarcinoma cell line (SGC-7901).


METHODS
Expression of COX-2 mRNA and protein were measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blot, PGE2 was determined by radioimmunoassay (RIA).


RESULTS
At the concentrations of 25, 50, 100, 200 pmol/L, c9, t11-CLA suppressed the expression of COX-2 mRNA, protein and PGE.


CONCLUSIONS
COX-2 is involved anti-cancer action of c9, t11-CLA and is likely to act as an important target.
Zhang
Jing-Shu
JS
School of Public Health, Harbin Medical University, Harbin 150086, China.
Chen
Bing-Qing
BQ
Wang
Qi
Q
Yang
Yan-Mei
YM
Gao
Yanhui
Y
Sun
Wenguang
W
Song
Yang
Y
D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D015232:Dinoprostone / Q000378:metabolism; D006801:Humans; D019787:Linoleic Acid / Q000494:pharmacology; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000201:enzymology / Q000473:pathology*
D052246:Cyclooxygenase 2 Inhibitors; D044243:Linoleic Acids, Conjugated; D012333:RNA, Messenger; C046938:9,11-linoleic acid; D019787:Linoleic Acid; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D015232:Dinoprostone