A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation.
Phosphorus, one of the essential elements for plants, is often a limiting nutrient because of its low availability and mobility in soils. Significant changes in plant morphology and biochemical processes are associated with phosphate (Pi) deficiency. However, the molecular bases of these responses to Pi deficiency are not thoroughly elucidated. Therefore, a comprehensive survey of global gene expression in response to Pi deprivation was done by using Arabidopsis thaliana whole genome Affymetrix gene chip (ATH1) to quantify the spatio-temporal variations in transcript abundance of 22,810 genes. The analysis revealed a coordinated induction and suppression of 612 and 254 Pi-responsive genes, respectively. The functional classification of some of these genes indicated their involvement in various metabolic pathways, ion transport, signal transduction, transcriptional regulation, and other processes related to growth and development. This study is a detailed analysis of Pi starvation-induced changes in gene expression of the entire genome of Arabidopsis correlated with biochemical processes. The results not only enhance our knowledge about molecular processes associated with Pi deficiency, but also facilitate the identification of key molecular determinants for improving Pi use by crop species.
Misson
Julie
J
Laboratoire de Biologie du Développement des Plantes, Unite Mixte de Recherche 6191, Centre National de la Recherche Scientifique-Commissariat à l'Energie Atomique, Aix-Marseille II, 13108 Saint-Paul-lez-Durance, France.
Raghothama
Kashchandra G
KG
Jain
Ajay
A
Jouhet
Juliette
J
Block
Maryse A
MA
Bligny
Richard
R
Ortet
Philippe
P
Creff
Audrey
A
Somerville
Shauna
S
Rolland
Norbert
N
Doumas
Patrick
P
Nacry
Philippe
P
Herrerra-Estrella
Luis
L
Nussaume
Laurent
L
Thibaud
Marie-Christine
MC
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D010710:Phosphates / Q000172:deficiency* / Q000494:pharmacology; D015203:Reproducibility of Results; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics
D029681:Arabidopsis Proteins; D010710:Phosphates