title: MspI polymorphisms in the 3rd intron of the swine POU1F1 gene and their associations with growth performance.
abstract: The study aimed to compare MspI polymorphisms in the 3rd intron of the porcine gene encoding the pituitary-1 transcription factor (Pit-1, renamed as POU1F1) among 5 breeds and to determine the associations between its genotypes and growth performance in a commercial pig population by using the PCR-RFLP technique. Significant differences in genotypic and allelic frequencies were found between the meat-type and fat-type breeds (P < 0.05), and between miniature pigs and others (P < 0.05). No breed deviated from the Hardy-Weinberg equilibrium (verified by chi-square test). The general linear model analysis revealed that higher body weight on day 180 (BW180) and average daily gain (ADG) were significantly associated with POU1F1 DD genotype (P < 0.05). The differences in BW180 and ADG between DD pigs and both CD and CC pigs were significant (P < 0.05), and the DD pigs had a significantly higher body weight on day 45 (BW45) and on day 70 (BW70) than CC pigs (P < 0.05). All measured growth traits, except for body weight at birth (BWB), showed higher values in DD pigs. The D allele had a favorable positive effect on growth traits. Thus POU1F1 is a potential major gene or marker for growth traits.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou City, 12 Wenhui East Road, Jiangsu Province 225009, P.R. China. schengyi@hotmail.com
authors.forename: Chengyi
authors.initials: C
authors.lastname: Song
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.forename: Yong
authors.initials: Y
authors.lastname: Teng
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Zhiyue
authors.initials: Z
authors.lastname: Wang
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Li
authors.forename: Haifeng
authors.initials: H
authors.lastname: Mi
authors.forename: Rongbin
authors.initials: R
authors.lastname: Jing
authors.forename: Jiude
authors.initials: J
authors.lastname: Mao
mesh_terms: D000483:Alleles; D000818:Animals; D001835:Body Weight / Q000235:genetics*; D001947:Breeding; D019003:Deoxyribonuclease HpaII / Q000502:physiology*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D007438:Introns / Q000235:genetics*; D008297:Male; D008460:Meat; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000097:blood / Q000235:genetics* / Q000254:growth & development*; D014157:Transcription Factors / Q000235:genetics*; D015430:Weight Gain
chemical_list: D014157:Transcription Factors; D019003:Deoxyribonuclease HpaII