The C-terminal tail of the polycystin-1 protein interacts with the Na,K-ATPase alpha-subunit.
Polycystin-1 (PC-1) is the product of the PKD1 gene, which is mutated in autosomal dominant polycystic kidney disease. We show that the Na,K-ATPase alpha-subunit interacts in vitro and in vivo with the final 200 amino acids of the polycystin-1 protein, which constitute its cytoplasmic C-terminal tail. Functional studies suggest that this association may play a role in the regulation of the Na,K-ATPase activity. Chinese hamster ovary cells stably expressing the entire PC-1 protein exhibit a dramatic increase in Na,K-ATPase activity, although the kinetic properties of the enzyme remain unchanged. These data indicate that polycystin-1 may contribute to the regulation of Na,K-ATPase activity in kidneys in situ, thus modulating renal tubular fluid and electrolyte transport.
Zatti
Alessandra
A
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Chauvet
Veronique
V
Rajendran
Vanathy
V
Kimura
Thoru
T
Pagel
Phillip
P
Caplan
Michael J
MJ
D000818:Animals; D016466:CHO Cells / Q000648:ultrastructure; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004285:Dogs; D004791:Enzyme Inhibitors; D004925:Escherichia; D009154:Mutation; D010042:Ouabain / Q000494:pharmacology*; D007690:Polycystic Kidney Diseases; D011506:Proteins; D011994:Recombinant Proteins; D012964:Sodium / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D050396:TRPP Cation Channels; D014162:Transfection
D004791:Enzyme Inhibitors; D010042:Ouabain; D011506:Proteins; D011994:Recombinant Proteins; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D050396:TRPP Cation Channels