title: Intracellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein: absence of nucleolar accumulation during infection and after expression as a recombinant protein in vero cells.
abstract: The nucleocapsid (N) protein of several members within the order Nidovirales localizes to the nucleolus during infection and after transfection of cells with N genes. However, confocal microscopy of N protein localization in Vero cells infected with the severe acute respiratory syndrome coronavirus (SARS-CoV) or transfected with the SARS-CoV N gene failed to show the presence of N in the nucleoplasm or nucleolus. Amino acids 369 to 389, which contain putative nuclear localization signal (NLS) and nucleolar localization signal motifs, failed to restore nuclear localization to an NLS-minus mutant Rev protein. These data indicate that nuclear localization is not a conserved property among all nidoviruses.
authors.affiliation: Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, 66506, USA. browland@vet.ksu.edu
authors.forename: Raymond R R
authors.initials: RR
authors.lastname: Rowland
authors.forename: Vinita
authors.initials: V
authors.lastname: Chauhan
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.forename: Andrew
authors.initials: A
authors.lastname: Pekosz
authors.forename: Maureen
authors.initials: M
authors.lastname: Kerrigan
authors.forename: Miriam D
authors.initials: MD
authors.lastname: Burton
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D002522:Chlorocebus aethiops; D000086462:Coronavirus Nucleocapsid Proteins; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals / Q000378:metabolism; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D045473:Severe acute respiratory syndrome-related coronavirus / Q000502:physiology*; D014709:Vero Cells / Q000378:metabolism*
chemical_list: D000086462:Coronavirus Nucleocapsid Proteins; D019913:Nuclear Localization Signals; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins