[Study on the animal model for severe acute respiratory syndrome].
To screen small animals susceptible to SARS-CoV, five species of animals, including guinea pig, hamster, albino hamster, chicken and rat, were experimentally infected with SARS-CoV strain BJ-01 by different routes. On the basis of this, further cynomolgus and rhesus macaques were selected and experimentally inoculated SARS-CoV, the quality they serve as animal model for SARS was evaluated. The results showed that, all five species of small animals chosed were not susceptible to SARS-CoV, no characterized changes in clinical sign and histopathology were observed after infection, but from the lung samples of large rat and pig guinea, the genomic RNA of SARS-CoV could be detected by RT-PCR at day 14 post infection, this suggested that SARS-CoV could replicate in these animals. After inoculated with SARS-CoV, all inoculated cynomolgus and rhesus macaques had developed interstitial pneumonia of differing severity. These changes on histopathology were similar to that seen in SARS patients, but the pathological lesions were less severe than that of human. Except interstitial pneumonia, no other characterized pathological changes were observed. This suggested cynomolgus and rhesus macaques were not the ideal animal model for SARS in fact, but they could serve as animal model for SARS when a more ideal animal model is absent.
Liu
Bo-Hua
BH
Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China. bhl726915@sohu.com
Wu
Dong-Lai
DL
Zhan
Da-Wei
DW
Qin
E-De
ED
Zhu
Qing-Yu
QY
Wang
Cui-E
CE
Meng
Qing-Wen
QW
Peng
Wen-Ming
WM
Yin
Xun-Nan
XN
Yang
Yin-Hui
YH
Guan
Yun-Tao
YT
Han
Wei-Guo
WG
Li
Chang-Wen
CW
Liu
Yong-Gang
YG
Wang
Mou-Ping
MP
Liu
Quan-Gui
QG
Shi
Hui-Ying
HY
Ding
Zhi-Fen
ZF
D000818:Animals; D002645:Chickens; D004195:Disease Models, Animal; D006801:Humans; D008252:Macaca fascicularis; D008253:Macaca mulatta; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045169:Severe Acute Respiratory Syndrome / Q000473:pathology / Q000821:virology*; D014779:Virus Replication