Use of transgenic zebrafish reporter lines to study calcium signalling in development.
Calcium signals are associated with many of the events common to animal development. Understanding the role of these calcium signals requires the ability to visualise and manipulate calcium levels in the developing embryo. Recent work has led to the development of sensitive protein-based probes that can be used to generate transgenic animals for the analysis of calcium signalling in vivo. This paper focuses on the use of genetically encoded calcium probes to follow calcium signals in zebrafish. It reviews progress and speculates on the potential for use in the future.
Ashworth
Rachel
R
Department of Physiology, University College of London, London, UK.
Brennan
Caroline
C
D000818:Animals; D030801:Animals, Genetically Modified; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D004625:Embryo, Nonmammalian / Q000502:physiology; D047108:Embryonic Development; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000737:chemistry; D009474:Neurons / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D015027:Zebrafish
D008164:Luminescent Proteins; D002118:Calcium