Anti-inflammatory activity of Seabuckthorn (Hippophae rhamnoides) leaves.
Immunomodulatory activity of Seabuckthorn (SBT) leaf extract was evaluated in adjuvant induced arthritis (AIA) rat model. Inflammation was induced by injecting Complete Freund's Adjuvant (CFA) in the right hind paw of rats. SBT extract was administered intraperitoneally to treat the inflammation. The extent of inflammation and treatment response was evaluated by clinical analysis, scintigraphic visualization using technitium-99m-glutathione (Tc99m-GSH) and lymphocyte proliferation. Serial evaluation was carried out on days 1, 7, 14, 21 and 28 after creation of inflammation. The Tc99m-GSH uptake in the inflamed leg was compared with the normal contralateral leg of the same animal. The measurements were done by obtaining scintigraphic images using gamma camera and an online computer. Both qualitative and quantitative evaluation of radiotracer accumulation was considered to evaluate the anti-inflammatory response. The lymphocyte proliferation study revealed cellular immunosuppression during the early phase of the disease. Administration of SBT extract on the same day or 5 days prior to inflammatory insult into the joint, significantly reduced the inflammation as compared to the untreated animals in a dose dependent manner. These observations suggest that the SBT leaf extract has a significant anti-inflammatory activity and has the potential for the treatment of arthritis.
Ganju
Lilly
L
Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi-110054, India. lganju@rediffmail.com
Padwad
Yogendra
Y
Singh
Richa
R
Karan
Dev
D
Chanda
Sudipta
S
Chopra
Mohinder Kumar
MK
Bhatnagar
Parul
P
Kashyap
Ravi
R
Sawhney
Ramesh Chandra
RC
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005978:Glutathione / Q000031:analogs & derivatives; D031281:Hippophae / Q000737:chemistry*; D007111:Immunity, Cellular / Q000187:drug effects; D007274:Injections, Intraperitoneal; D007596:Joints / Q000000981:diagnostic imaging / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D010936:Plant Extracts / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D011877:Radionuclide Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D013667:Technetium
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; C090285:technetium Tc 99m glutathione; D013667:Technetium; D005978:Glutathione