title: Expression and modulation of LL-37 in normal human keratinocytes, HaCaT cells, and inflammatory skin diseases.
abstract: Defensins and cathelicidins (LL-37) are major antimicrobial peptides (AMPs) of the innate immune system of the human skin. In normal non-inflamed skin these peptides are negligible, but their expression can be markedly increased in inflammatory skin disease such as psoriasis. We designed this study to identify the expressions of LL-37 in normal human keratinocyte (NHK) and HaCaT cells after exposure to stimulants and to investigate difference of LL-37 expression accompanied with cell differentiation status, and come to understand difference of susceptibility to infection in atopic dermatitis and psoriasis. Expressions of LL-37 in NHKs and HaCaT cells were evaluated by using RT-PCR, Western blotting, and immunohistochemical (IHC) staining at 6, 12, and 24 hr post stimulation after exposure to Ultraviolet B irradiation and lipopolysaccharide. And expression of LL-37 in skin biopsy specimens from patients with atopic dermatitis and psoriasis was determined by immunohistochemical analysis. In time-sequential analyses of LL-37 expression revealed that LL-37 was expressed in NHKs, but not in HaCaT cells. IHC analysis confirmed the presence of abundant LL-37 in the epidermis of psoriasis. Therefore we deduced that expression of LL-37 is affected by UV irradiation, bacterial infection, and status of cell differentiation.
authors.affiliation: Department of Dermatology, College of Medicine, Chung Ang University, Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Beom Joon
authors.initials: BJ
authors.lastname: Kim
authors.forename: Mi Sook
authors.initials: MS
authors.lastname: Jeong
authors.forename: Seong Jun
authors.initials: SJ
authors.lastname: Seo
authors.forename: Myeung Nam
authors.initials: MN
authors.lastname: Kim
authors.forename: Chang Kwun
authors.initials: CK
authors.lastname: Hong
authors.forename: Byung In
authors.initials: BI
authors.lastname: Ro
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000032:analysis / Q000235:genetics*; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D023082:Defensins / Q000032:analysis / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology / Q000378:metabolism; D012871:Skin Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D023082:Defensins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D054804:Cathelicidins