Generation and characterization of the PKC gamma-Cre mouse line.
PKCgamma is a protein kinase C (PKC) isoform that is expressed in the central nervous system (CNS). We generated a PKCgamma-Cre mouse line and characterized the expression and activity of the Cre protein. Our studies show that Cre expression largely recapitulates the endogenous expression of PKCgamma. Several major sites of Cre expression are the cerebral cortex, hippocampus, thalamus, amygdala, and spinal cord. Examination of PKCgamma-Cre/ROSA26 mice reveals a similar X-gal staining pattern in the CNS, indicating that Cre recombinase is capable of removing LoxP sites in vivo. These data indicate that the PKCgamma-Cre mouse line could be a useful reagent for generating Cre-mediated tissue-specific knockouts in the CNS.
Ding
Yu-Qiang
YQ
Department of Anesthesiology, School of Medicine,Washington University Pain Center, St. Louis, Missouri 63110, USA.
Xiang
Chuan-Xi
CX
Chen
Zhou-Feng
ZF
D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology; D017930:Genes, Reporter; D007150:Immunohistochemistry; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D011493:Protein Kinase C / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated; D013116:Spinal Cord / Q000201:enzymology
C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D011506:Proteins; D022661:RNA, Untranslated; C087857:protein kinase C gamma; D011493:Protein Kinase C; C045073:Cre recombinase; D019426:Integrases