[Isolation and identification Alicyclobacillus from concentrated apple juice processing].
The forty-five strains of bacteria have been isolated from concentrated apple juice. All of them can grow at K agar (pH 4.0), but can't grow at LB palate (pH 7.0). By using heat resistance test, 38 strains of these bacteria could be classified into 3 groups according to their spore-forming time. 33 strains of them had the same spore-forming time as DSM 3922, which could produce spores within 48 h when cultivated at 45 degrees C; 3 strains of them could grow very fast and produced spores within 17 h; and 3 strains of them produced spores 48h later. Based on the results of 16S rDNA PCR-RFLP analysis, 7 strains of unknown, probably new species and 5 reference strains were selected for 19 phenotypic characteristics test and the result were analyses by UPGMA. The results of phenotypic characteristics test had good agreement with 16S rDNA PCR-RFLP analyses, that the 7 strains were different from all the 5 reference strains, they are new species of Alicyclobacillus.
Chen
Shi-Qiong
SQ
College of Food Science and Nutrition Engineering, China Agriculture University, Beijing 100083, China. shiqiongchen@sohu.com
Hu
Xiao-Song
XS
Shi
Wei-Ni
WN
Dong
Jian-Cheng
JC
Wang
Jian
J
D001628:Beverages / Q000382:microbiology*; D005511:Food Handling; D016937:Gram-Positive Endospore-Forming Rods / Q000302:isolation & purification* / Q000502:physiology; D027845:Malus / Q000382:microbiology; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013171:Spores, Bacterial / Q000502:physiology
D012336:RNA, Ribosomal, 16S