[Immunological mechanism in development of allergic dermatitis in guinea pig induced by trichloroethylene in vitro].
OBJECTIVE
To investigate the immunological mechanism of allergic dermatitis induced by trichloroethylene (TCE).


METHODS
The guinea pig model of TCE-induced allergic dermatitis was established by Guinea pig Maximization Test. The effects of TCE and its metabolites on splenic lymphocytes of TCE-sensitized and non-sensitized guinea pig were detected by MTT assay.


RESULTS
For TCE-sensitized guinea pig, the survival rate of lymphocytes cultured with TCE (+S9) was significantly higher than that cultured with TCE (-S9) (83.0% +/- 3.4% vs 75.9% +/- 7.9%, P < 0.01), while, for normal animals, the survival rate of lymphocytes cultured with TCE (+S9) was significantly lower than that cultured with TCE (-S9) (63.4% +/- 8.4% vs 77.0% +/- 7.2%, P < 0.01). The survival rate of lymphocytes cultured with TCE (+S9) in TCE-sensitized animals was higher than that in normal animals (83.0% +/- 3.4% vs 63.4% +/- 8.4%, P < 0.05), but no statistically significant difference was found for TCE (-S9) (75.9% +/- 7.9% vs 77.0% +/- 7.2%, P > 0.05).


CONCLUSIONS
Cytotoxicity of TCE to normal lymphocytes and proliferation of sensitized lymphocytes were enhanced by metabolic activation. The metabolites of TCE may act as effective immune hapten to stimulate the proliferation of hapten-specific lymphocytes in TCE-sensitized animals.
Dai
Yu-fei
YF
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Niu
Yong
Y
Cheng
Juan
J
Leng
Shu-guang
SG
Zheng
Yu-xin
YX
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017449:Dermatitis, Allergic Contact / Q000209:etiology / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D013154:Spleen / Q000187:drug effects* / Q000276:immunology; D014241:Trichloroethylene / Q000633:toxicity*
D014241:Trichloroethylene