title: Export-mediated assembly of mycobacterial glycoproteins parallels eukaryotic pathways.
abstract: Protein O-mannosylation is an essential and evolutionarily conserved post-translational modification among eukaryotes. This form of protein modification is also described in Mycobacterium tuberculosis; however, the mechanism of mannoprotein assembly remains unclear. Evaluation of differentially translocated chimeric proteins and mass spectrometry to monitor glycosylation demonstrated that specific translocation processes were required for protein O-mannosylation in M. tuberculosis. Additionally, Rv1002c, a M. tuberculosis membrane protein homolog of eukaryotic protein mannosyltransferases, was shown to catalyze the initial step of protein mannosylation. Thus, the process of protein mannosylation is conserved between M. tuberculosis and eukaryotic organisms.
authors.affiliation: Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA.
authors.forename: Brian C
authors.initials: BC
authors.lastname: VanderVen
authors.forename: Jeffery D
authors.initials: JD
authors.lastname: Harder
authors.forename: Dean C
authors.initials: DC
authors.lastname: Crick
authors.forename: John T
authors.initials: JT
authors.lastname: Belisle
mesh_terms: D003001:Cloning, Molecular; D005057:Eukaryotic Cells / Q000378:metabolism; D019143:Evolution, Molecular; D006031:Glycosylation; D008358:Mannose / Q000378:metabolism; D008364:Mannosyltransferases / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D020102:Mycobacterium smegmatis / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; C025363:mannoproteins; D008364:Mannosyltransferases; C082696:protein O-mannosyltransferase; D008358:Mannose