[Studies on the extraction and purification of total saponins from Parched Semen Ziziphi Spinosae].
OBJECTIVE
To study the extraction and purification process of the total saponin from Parched Semen Ziziphi Spinosae with ethanol and macroporous resin.


METHODS
The total saponins were extracted with ethanol and purified with macroporous resin by orthogonal design, taking content and purity of jujuboside A as guideline.


RESULTS
The optimum extraction condition was adding 6 times amount of 80% ethanol and refluxing 3 times, for 30 minutes each time. The purification process with macroporous resin HPD-100 was using 0.5% NaOH (150ml), 30% ethanol (150ml) to wash out impurity, and 70% ethanol 50 ml to wash out saponin. The purity of jujuboside A was up to 17.9% and the eluted ratio 72.8%.
Wu
Yulan
Y
Nanjing University of TCM.
Ding
Anwei
A
Bao
Beihua
B
D000704:Analysis of Variance; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D031957:Ziziphus / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D012117:Resins, Synthetic; D012503:Saponins; D000431:Ethanol