title: Gelatin binding to the 8F19F1 module pair of human fibronectin requires site-specific N-glycosylation.
abstract: The gelatin (denatured collagen) binding domain of the extracellular matrix protein fibronectin contains three potential N-glycosylation sites. Complete deglycosylation of this domain is known to reduce the thermal stability of the eighth type 1 (8F1) module. We have conducted a site-specific analysis of the structural and functional consequences of N-linked glycosylation in the 8F19F1 module pair. Three glycoforms have been identified by mass spectrometry and nuclear magnetic resonance spectroscopy. Chemical shift differences between the glycoforms have revealed an intimate interaction between one N-linked sugar and the polypeptide that is critical for gelatin binding, as shown by affinity chromatography.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Millard
authors.forename: Iain D
authors.initials: ID
authors.lastname: Campbell
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Pickford
mesh_terms: D000595:Amino Acid Sequence; D001216:Asparagine / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D005353:Fibronectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D005780:Gelatin / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010843:Pichia / Q000201:enzymology; D017354:Point Mutation; D043484:Proprotein Convertases / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary
chemical_list: D005353:Fibronectins; D001216:Asparagine; D005780:Gelatin; D043484:Proprotein Convertases