Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2.
IF2 is one of three bacterial translation initiation factors that are conserved through all kingdoms of life. It binds the 30S and 50S ribosomal subunits, as well as fMet-tRNAf(Met). After these interactions, fMet-tRNAf(Met) is oriented to the ribosomal P-site where the first amino acid of the nascent polypeptide, formylmethionine, is presented. The C-terminal domain of Bacillus stearothermophilus IF2, which is responsible for recognition and binding of fMet-tRNAf(Met), contains two structured modules. Previously, the solution structure of the most C-terminal module, IF2-C2, has been elucidated by NMR spectroscopy and direct interactions between this subdomain and fMet-tRNAf(Met) were reported. In the present NMR study we have obtained the spectral assignment of the other module of the C-terminal domain (IF2-C1) and determined its solution structure and backbone dynamics. The IF2-C1 core forms a flattened fold consisting of a central four-stranded parallel beta-sheet flanked by three alpha-helices. Although its overall organization resembles that of subdomain III of the archaeal IF2-homolog eIF5B whose crystal structure had previously been reported, some differences of potential functional significance are evident.
Wienk
Hans
H
Bijvoet Center for Biomolecular Research, Department of NMR Spectroscopy, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Tomaselli
Simona
S
Bernard
Cédric
C
Spurio
Roberto
R
Picone
Delia
D
Gualerzi
Claudio O
CO
Boelens
Rolf
R
D015852:Eukaryotic Initiation Factor-2 / Q000737:chemistry* / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010448:Peptide Initiation Factors / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012996:Solutions; D040681:Structural Homology, Protein
D015852:Eukaryotic Initiation Factor-2; C118735:IF2 protein, archaeal; D010448:Peptide Initiation Factors; D012996:Solutions