title: Habitual exercise induced resistance to oxidative stress.
abstract: We investigated whether habitual exercise (HE) modulates levels of oxidative DNA damage and responsiveness to oxidative stress induced by renal carcinogen Fe-nitrilotriacetic acid (Fe-NTA). During a ten week protocol, two groups of rats either remained sedentary or underwent swimming for 15--60 min per day, 5 days per week, with or without a weight equivalent to 5% of their body weight. Then we injected Fe-NTA and sacrificed the rats 1 h after the injection. We determined the activity of superoxide dismutase (SOD) in diaphragm and kidney, evaluated levels of 8-hydroxydeoxyguanosine (8OHdG), catalase, and glutathione peroxidase, and assayed OGG1 protein levels in kidney. SOD activity in the diaphragm and kidney was increased in HE rats. By itself, HE had no effect on the level of 8OHdG, but it did significantly suppress induction of 8OHdG by Fe-NTA, and the amount of suppression correlated with intensity of exercise. These results suggest that HE induces resistance to oxidative stress and, at least at the initiation stage, inhibits carcinogenesis.
authors.affiliation: Department of Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, Sakuragaoka, Kagoshima, 890-8544, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Nakatani
authors.forename: Masaharu
authors.initials: M
authors.lastname: Komatsu
authors.forename: Toyohiro
authors.initials: T
authors.lastname: Kato
authors.forename: Takao
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takekura
authors.forename: Akira
authors.initials: A
authors.lastname: Wagatsuma
authors.forename: Kohji
authors.initials: K
authors.lastname: Aoyama
authors.forename: Baohui
authors.initials: B
authors.lastname: Xu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hirano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kasai
authors.forename: Seiichi
authors.initials: S
authors.lastname: Ando
authors.forename: Toru
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001835:Body Weight; D002273:Carcinogens; D004249:DNA Damage / Q000502:physiology*; D045647:DNA Glycosylases / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D003964:Diaphragm / Q000201:enzymology / Q000378:metabolism; D005290:Ferric Compounds; D007668:Kidney / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D005082:Physical Exertion; D051381:Rats; D013550:Swimming
chemical_list: D002273:Carcinogens; D005290:Ferric Compounds; D000080242:8-Hydroxy-2'-Deoxyguanosine; D045647:DNA Glycosylases; C483741:OGG1 protein, rat; D003849:Deoxyguanosine; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate