[The comparative study of the effect of new fluorine-containing guanidine-derivate compounds and pinacidil on contractile function of the urinary bladder in vitro].
The effects of the new fluorine-containing guanidine-derivate compounds on bladder contractile functionwere examined on isolated bladder strips in vitro. Female rats (250-350 g) were used. The bladder was removed and placed in the room temperaturePSS of the following composition (mM): NaCl (118.4), KCl (4.7),CaCl2 (2.5), MgSO4 (1.2), KH2PO4 (1.2), NaHCO3 (24.9), and D-glucose (11.1) gassed with O2-CO2. 95%-5%. The base of the bladder was then cut into strips 4-to 5-mm wide and 10-mm long. Then strips were pretensioned (0.25-0.5 g) and after 30 min of equilibration were contracted with an additional 60 mM KCl and again were allowed to equilibrate. Compounds were administered directly into the tissue bath. All compounds produced a concentration-dependent relaxation of isolated rat bladder strips.
Lymarenko
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D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D019791:Guanidine / Q000031:analogs & derivatives / Q000494:pharmacology*; D006845:Hydrocarbons, Fluorinated / Q000494:pharmacology*; D009126:Muscle Relaxation / Q000187:drug effects*; D009924:Organ Culture Techniques; D020110:Pinacidil / Q000031:analogs & derivatives / Q000494:pharmacology*; D015221:Potassium Channels / Q000378:metabolism; D051381:Rats; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism
D006845:Hydrocarbons, Fluorinated; D015221:Potassium Channels; D020110:Pinacidil; D000255:Adenosine Triphosphate; D019791:Guanidine