Analysis of genes involved in methyl halide degradation in Aminobacter lissarensis CC495.
Aminobacter lissarensis CC495 is an aerobic facultative methylotroph capable of growth on glucose, glycerol, pyruvate and methylamine as well as the methyl halides methyl chloride and methyl bromide. Previously, cells grown on methyl chloride have been shown to express two polypeptides with apparent molecular masses of 67 and 29 kDa. The 67 kDa protein was purified and identified as a halomethane:bisulfide/halide ion methyltransferase. This study describes a single gene cluster in A. lissarensis CC495 containing the methyl halide utilisation genes cmuB, cmuA, cmuC, orf 188, paaE and hutI. The genes correspond to the same order and have a high similarity to a gene cluster found in Aminobacter ciceronei IMB-1 and Hyphomicrobium chloromethanicum strain CM2 indicating that genes encoding methyl halide degradation are highly conserved in these strains.
Warner
Karen L
KL
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
Larkin
Michael J
MJ
Harper
David B
DB
Murrell
J Colin
JC
McDonald
Ian R
IR
D020561:Alphaproteobacteria / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D005798:Genes, Bacterial; D006842:Hydrocarbons, Brominated / Q000378:metabolism*; D020582:Hyphomicrobium / Q000235:genetics; D008737:Methyl Chloride / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D026801:Synteny
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006842:Hydrocarbons, Brominated; C005218:methyl bromide; D008737:Methyl Chloride