title: Glycosaminoglycans modulate C6 glioma cell adhesion to extracellular matrix components and alter cell proliferation and cell migration.
abstract: BACKGROUND
  Adhesion to extracellular matrix (ECM) components has been implicated in the proliferative and invasive properties of tumor cells. We investigated the ability of C6 glioma cells to attach to ECM components in vitro and described the regulatory role of glycosaminoglycans (GAGs) on their adhesion to the substrate, proliferation and migration.
  
  
  RESULTS
  ECM proteins (type IV collagen, laminin and fibronectin) stimulate rat C6 glioma cell line adhesion in vitro, in a dose-dependent manner. The higher adhesion values were achieved with type IV collagen. Exogenous heparin or chondroitin sulfate impaired, in a dose-dependent manner the attachment of C6 glioma cell line to laminin and fibronectin, but not to type IV collagen. Dextran sulfate did not affect C6 adhesion to any ECM protein analyzed, indicating a specific role of GAGs in mediating glioma adhesion to laminin and fibronectin. GAGs and dextran sulfate did not induce C6 glioma detachment from any tested substrate suggesting specific effect in the initial step of cell adhesion. Furthermore, heparin and chondroitin sulfate impaired C6 cells proliferation on fibronectin, but not on type IV collagen or laminin. In contrast, both GAGs stimulate the glioma migration on laminin without effect on type IV collagen or fibronectin.
  
  
  CONCLUSIONS
  The results suggest that GAGs and proteoglycans regulate glioma cell adhesion to ECM proteins in specific manner leading to cell proliferation or cell migration, according to the ECM composition, thus modulating tumor cell properties.
authors.affiliation: Departamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, Brazil. atrentin@ccb.ufsc.br
authors.forename: Claudia Beatriz Nedel Mendes de
authors.initials: CB
authors.lastname: Aguiar
authors.forename: Bruno
authors.initials: B
authors.lastname: Lobão-Soares
authors.forename: Marcio
authors.initials: M
authors.lastname: Alvarez-Silva
authors.forename: Andréa Gonçalves
authors.initials: AG
authors.lastname: Trentin
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D024141:Collagen Type IV / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000494:pharmacology / Q000502:physiology*; D005353:Fibronectins / Q000494:pharmacology; D005910:Glioma / Q000473:pathology*; D006025:Glycosaminoglycans / Q000494:pharmacology*; D007797:Laminin / Q000494:pharmacology; D009361:Neoplasm Invasiveness; D011509:Proteoglycans / Q000494:pharmacology; D051381:Rats
chemical_list: D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006025:Glycosaminoglycans; D007797:Laminin; D011509:Proteoglycans