[Effect of 5-fluorouracil on DNA synthesis, mitotic index and spleen cells in mice after syngeneic bone marrow transplantation].
The effects of 5-fluorouracil (5-FU) on DNA synthesis, mitotic index and cell composition of mouse spleens after 6 Gy X rays irradiation of mice and syngenic bone marrow transplantation were investigated. One dose of 5-FU (100 mg/kg body weight) administered 16 hrs after bone marrow transplantation reduced mitotic index and development of bone marrow grafts, but did not inhibit incorporation of 3H-thymidine into DNA. It correlates with high thymidylate synthase activity previously noted in the same model. Also the increased number of cells from lineage of erythroblasts correlates with high DNA synthesis and thymidylate synthase activity indicating, probably, the presence in those cell lines, the cells with high thymidylate synthase content, resistant to 5-FU.
Panfil
B
B
Zakład Radiobiologii, Wojskowy Instytut Higieny i Epidemiologii, ul. Kozielska 4, 01-163 Warszawa.
Chomiczewski
K
K
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology*; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D016026:Bone Marrow Transplantation; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D005472:Fluorouracil / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008938:Mitosis / Q000187:drug effects*; D008940:Mitotic Index; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D014185:Transplantation, Isogeneic
D000963:Antimetabolites; D000964:Antimetabolites, Antineoplastic; D004247:DNA; D005472:Fluorouracil