title: Mass spectrometric analysis of histone posttranslational modifications.
abstract: The uses of tandem and Fourier transform mass spectrometric methodologies for assignment of the posttranslational sites and occupancies of histones and their isoforms is described employing several illustrative examples. A comparison of information that can be obtained from intact protein sequencing and proteolytic digestion is presented.
authors.affiliation: Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143-0446, USA. alb@itsa.ucsf.edu
authors.forename: A L
authors.initials: AL
authors.lastname: Burlingame
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Chalkley
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005583:Fourier Analysis; D006657:Histones / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007477:Ions / Q000737:chemistry; D008239:Lysine / Q000378:metabolism; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D008745:Methylation; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods; D014357:Trypsin / Q000378:metabolism
chemical_list: D006657:Histones; D007477:Ions; D010446:Peptide Fragments; D014357:Trypsin; D008239:Lysine