Molecular serotyping of Escherichia coli O26:H11.
Serotyping is the foundation of pathogenic Escherichia coli diagnostics; however, few laboratories have this capacity. We developed a molecular serotyping protocol that targets, genetically, the same somatic and flagellar antigens of E. coli O26:H11 used in traditional serotyping. It correctly serotypes strains untypeable by traditional methods, affording primary laboratories serotyping capabilities.
Durso
Lisa M
LM
U.S. Meat Animal Research Center, U.S. Department of Agriculture/ARS, P.O. Box 166, State Spur 18D, Clay Center, NE 68933, USA.
Bono
James L
JL
Keen
James E
JE
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D015373:Bacterial Typing Techniques; D002417:Cattle; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005408:Flagellin / Q000235:genetics; D008969:Molecular Sequence Data; D019081:O Antigens / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D012703:Serotyping
D000942:Antigens, Bacterial; C022863:H antigen; D019081:O Antigens; D005408:Flagellin