title: Bioactive and biocompatible pieces of HA/sol-gel glass mixtures obtained by the gel-casting method.
abstract: Hydroxyapatite (HA)/glass mixtures have shown a faster bioactive behaviour than HA itself. On the other hand, the gel-casting method is a simple and reproducible colloidal method to produce ceramic pieces with complex shapes. In this work, pieces of HA/glass mixtures were prepared by the gel-casting method. A study for obtaining concentrated slurries of these mixtures is reported; the bioactivity and biocompatibility of the obtained pieces have been studied also. The influence of pH, dispersant concentration, the content and milling of glass, and the way to prepare the suspensions were investigated. The lowest viscosity and better rheological properties were achieved with the lowest glass content, when the glass was added after the dispersion of the HA powder and when the glass was not milled after calcination. Fluid suspensions with a high solid content (50 vol.%) could be prepared and well-shaped pieces were obtained from these slurries. These pieces showed in vitro bioactive behavior in simulated body fluid; additionally, the proliferation and spreading assays with osteoblastic cells (HOS) showed that the pieces are biocompatible. The results obtained indicate that the gel-casting of HA/glass mixtures produces bioactive and biocompatible pieces with the required shapes. Therefore, these materials could be good candidates for clinical applications and scaffolds for tissue engineering.
authors.affiliation: Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Padilla
authors.forename: S
authors.initials: S
authors.lastname: Sánchez-Salcedo
authors.forename: M
authors.initials: M
authors.lastname: Vallet-Regí
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D017886:Durapatite / Q000737:chemistry*; D004586:Electrophoresis; D005898:Glass / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000737:chemistry / Q000378:metabolism; D011208:Powders; D012212:Rheology; D013314:Stress, Mechanical; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D014783:Viscosity; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D006882:Hydroxyapatites; D011208:Powders; D017886:Durapatite