The protein composition of honeybee venom reconsidered by a proteomic approach.
Pure honeybee venom samples were submitted to two-dimensional gel electrophoresis. A total of 49 excised spots were analyzed by mass spectrometry; 39 of them resulted in the identification of 6 different known bee venom proteins and of 3 proteins that have not been described in such samples before. The first new venom protein has a platelet-derived and vascular endothelial growth factor family domain, the second protein shows no homologies with any known protein and the third matches a hypothetical protein similar to major royal jelly protein 8.
Peiren
Nico
N
Laboratory of Zoophysiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Vanrobaeys
Frank
F
de Graaf
Dirk C
DC
Devreese
Bart
B
Van Beeumen
Jozef
J
Jacobs
Frans J
FJ
D000595:Amino Acid Sequence; D000818:Animals; D001514:Bee Venoms / Q000737:chemistry* / Q000302:isolation & purification; D001516:Bees; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D019476:Insect Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D020543:Proteome
D001514:Bee Venoms; D019476:Insect Proteins; D010446:Peptide Fragments; D020543:Proteome