Recognition of dengue virus protein using epitope-mediated molecularly imprinted film.
Molecularly imprinted film was fabricated in the presence of a pentadecapeptide onto a quartz crystal microbalance (QCM) chip. This 15-mer peptide has been known as the linear epitope of the dengue virus NS1 protein. Imprinting resulted in an increased polymer affinity toward the corresponding templates but also to the virus protein. Direct detection of the dengue virus protein was achieved quantitatively. The QCM chip response to the NS1 protein was obtained using epitope-mediated imprinting demonstrating a comparable frequency shift in chips immobilized with monoclonal antibodies. The binding effect was further enhanced and confirmed using a monoclonal antibody to form a sandwich with the MIP-NS1 protein complex on the chip. No pretreatment was required.
Tai
Dar-Fu
DF
Department of Chemistry and Institute of Biotechnology, National Dong-Hwa University, Hualien, Taiwan. dftai@mail.ndhu.edu.tw
Lin
Chung-Yin
CY
Wu
Tzong-Zeng
TZ
Chen
Li-Kuang
LK
D000818:Animals; D040761:Biomimetic Materials; D003716:Dengue Virus / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D046208:Microchip Analytical Procedures; D051381:Rats; D017361:Viral Nonstructural Proteins / Q000032:analysis* / Q000276:immunology*
D000939:Epitopes; D017361:Viral Nonstructural Proteins