title: Analytical methods for assay of ellagic acid and its solubility studies.
abstract: Ultra-violet (UV) spectrophotometric and high performance liquid chromatographic methods for quantitative determination of ellagic acid (EA), an antioxidant were developed. The analytical methods were validated for linearity, accuracy, intra- and inter-day variability, and precision. EA was eluted using a polyethylene glycol (PEG) column with mobile phase composed of acetonitrile and 5 mM potassium dihydrogen orthophosphate buffer pH 2.5 (80:20, v/v). The UV and high performance liquid chromatography (HPLC) methods have lower detection limits of 0.2 and 0.1 microg/ml, respectively. Because of the increasing pharmaceutical interest in phytochemicals and their solubility problems, solubility studies for EA were also carried out. Various organic solvents and surfactants were screened for assessing solubility of EA and the compound was found to be soluble in some pharmaceutically acceptable solvents like triethanolamine, polyethylene glycol 400 and N-methyl pyrrollidone (NMP). Aqueous and pH dependent solubility of EA were determined using UV and HPLC methods, respectively.
authors.affiliation: Department of pharmaceutics, National Institute of Pharmaceutical Education and Research, NIPER, Sector 67, SAS Nagar, Phase X Mohali 160062, Punjab, India.
authors.forename: I
authors.initials: I
authors.lastname: Bala
authors.forename: V
authors.initials: V
authors.lastname: Bhardwaj
authors.forename: S
authors.initials: S
authors.lastname: Hariharan
authors.forename: M N V Ravi
authors.initials: MN
authors.lastname: Kumar
mesh_terms: D000975:Antioxidants / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004304:Dosage Forms; D004610:Ellagic Acid / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D004364:Pharmaceutical Preparations; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility; D012997:Solvents / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D013997:Time Factors; D014466:Ultraviolet Rays; D014867:Water
chemical_list: D000975:Antioxidants; D004304:Dosage Forms; D004364:Pharmaceutical Preparations; D012997:Solvents; D014867:Water; D004610:Ellagic Acid