title: Lipid peroxides and antioxidant enzymes in cisplatin-induced chronic nephrotoxicity in rats.
abstract: Cisplatin (CDDP), an anticancer drug, induces remarkable toxicity in the kidneys of animals and humans and it has been well documented that reactive oxygen species and the renal antioxidant system are strongly involved in acute renal damage induced by CDDP. The aim of the present study was to investigate whether or not the renal antioxidant system plays also an important role in chronic renal damage induced by repeated doses of CDDP (1 mg/kg intraperitoneally twice weekly during 10 weeks in rats). In order to elucidate it, serum creatinine and urea levels, renal glutathione and thiobarbituric acid-reactive substances (TBARS) content, as well as renal superoxide dismutase and glutathione peroxidase activities were measured in the kidney homogenates of chronically CDDP-treated rats and additionally histological studies were performed in the rat kidneys. The chronic treatment with CDDP induced a significant increase in creatinine and urea levels in serum, but the other parameters mentioned above were not significantly modified as compared to the values in nontreated rats. Taking into account these results, we conclude that chronic CDDP administration induces also severe nephrotoxicity, in contrast to CDDP acute application, without any significant modification in the activity of relevant antioxidant enzymes such as superoxide dismutase and glutathione peroxidase, renal glutathione and lipid peroxides, by which the role of the antioxidant system in chronic nephrotoxicity induced by CDDP in rats is uncertain.
authors.affiliation: Department of Biomedicine, Ozone Research Center, National Center for Scientific Research, Havana, Cuba. ozono@infomed.sld.cu
authors.forename: Ricardo
authors.initials: R
authors.lastname: González
authors.forename: Cheyla
authors.initials: C
authors.lastname: Romay
authors.forename: Aluet
authors.initials: A
authors.lastname: Borrego
authors.forename: Frank
authors.initials: F
authors.lastname: Hernández
authors.forename: Nelson
authors.initials: N
authors.lastname: Merino
authors.forename: Zullyt
authors.initials: Z
authors.lastname: Zamora
authors.forename: Enis
authors.initials: E
authors.lastname: Rojas
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000633:toxicity*; D000975:Antioxidants / Q000378:metabolism*; D002945:Cisplatin / Q000008:administration & dosage / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014020:Tissue Extracts / Q000737:chemistry; D014508:Urea / Q000097:blood
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D014020:Tissue Extracts; D014508:Urea; D013482:Superoxide Dismutase; D005978:Glutathione; D002945:Cisplatin