The 2b protein as a minor structural component of PRRSV.
Porcine reproductive and respiratory syndrome virus (PRRSV) ORF2 contains an internal ORF that codes for a small non-glycosylated protein known as 2b. Previous work had identified the presence of a 10kDa 2b protein in virus-infected cells and the induction of an anti-2b response in PRRSV-infected pigs, as well as a possible association of 2b with the virion (, Virology 287:183-191). In this study, we utilized two experimental approaches, including the use of a 2b peptide-specific monoclonal antibody, to demonstrate that the PRRSV 2b protein is an integral component of the PRRSV virion. This study suggests that 2b in PRRSV is similar to the E protein in EAV and forms a minor structural component of the virion.
Wu
Wai-Hong
WH
Department of Veterinary Science, South Dakota State University, Box 2175, Brookings, SD 57007, USA.
Fang
Ying
Y
Rowland
Raymond R R
RR
Lawson
Steven R
SR
Christopher-Hennings
Jane
J
Yoon
Kyoung-Jin
KJ
Nelson
Eric A
EA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D047468:Immunoprecipitation; D019316:Porcine respiratory and reproductive syndrome virus / Q000737:chemistry* / Q000378:metabolism; D015678:Viral Structural Proteins / Q000276:immunology / Q000378:metabolism*; D014771:Virion / Q000737:chemistry* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015678:Viral Structural Proteins