title: Iron-mediated free-radical formation of signaling lipids in a model system.
abstract: It has been shown using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and its combination with thin-layer chromatography (TLC) that the action of the ascorbate/Fe(2+)/H(2)O(2) oxidizing system on cardiolipin and galactocerebroside results in the formation of phosphatidic acid (PA) and ceramide (Cer), respectively. These data, when combined with results obtained on radiolysis of similar substances, allowed the conclusion that the formation of PA and Cer occurs via an OH-induced fragmentation taking place in polar moiety of the starting substrates.
authors.affiliation: Department of Organic Chemistry, Belarusian State University, Minsk, Belarus.
authors.forename: Irina
authors.initials: I
authors.lastname: Yurkova
authors.forename: Mikhail
authors.initials: M
authors.lastname: Kisel
authors.forename: Juergen
authors.initials: J
authors.lastname: Arnhold
authors.forename: Oleg
authors.initials: O
authors.lastname: Shadyro
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry; D002308:Cardiolipins / Q000737:chemistry*; D002518:Ceramides / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D005296:Ferrous Compounds / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D005699:Galactosylceramides / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D008823:Micelles; D010084:Oxidation-Reduction; D010712:Phosphatidic Acids / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002308:Cardiolipins; D002518:Ceramides; D005296:Ferrous Compounds; D005609:Free Radicals; D005699:Galactosylceramides; D008823:Micelles; D010712:Phosphatidic Acids; C002454:galactocerebroside; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid