title: A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters.
abstract: Acyl-CoA-dependent O-acyltransferases catalyze reactions in which fatty acyl-CoAs are joined to acyl acceptors containing free hydroxyl groups to produce neutral lipids. In this report, we characterize a human multifunctional O-acyltransferase (designated MFAT) that belongs to the acyl-CoA:diacylglycerol acyltransferase 2/acyl-CoA:monoacylglycerol acyltransferase (MGAT) gene family and is highly expressed in the skin. Membranes of insect cells and homogenates of mammalian cells overexpressing MFAT exhibited significantly increased MGAT, acyl-CoA:fatty acyl alcohol acyltransferase (wax synthase), and acyl-CoA:retinol acyltransferase (ARAT) activities, which catalyze the synthesis of diacylglycerols, wax monoesters, and retinyl esters, respectively. Furthermore, when provided with the appropriate substrates, intact mammalian cells overexpressing MFAT accumulated more waxes and retinyl esters than control cells. We conclude that MFAT is a multifunctional acyltransferase that likely plays an important role in lipid metabolism in human skin.
authors.affiliation: Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA. eyen@gladstone.ucsf.edu
authors.forename: Chi-Liang Eric
authors.initials: CL
authors.lastname: Yen
authors.forename: Charles H
authors.initials: CH
authors.lastname: Brown
authors.forename: Mara
authors.initials: M
authors.lastname: Monetti
authors.forename: Robert V
authors.initials: RV
authors.lastname: Farese
mesh_terms: D000217:Acyltransferases / Q000737:chemistry* / Q000502:physiology*; D000818:Animals; D015152:Blotting, Northern; D019556:COS Cells; D002384:Catalysis; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004952:Esters / Q000737:chemistry*; D005989:Glycerides / Q000737:chemistry*; D006801:Humans; D015151:Immunoblotting; D007313:Insecta; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D010802:Phylogeny; D051142:Retinol O-Fatty-Acyltransferase / Q000737:chemistry; D012867:Skin / Q000378:metabolism*; D014018:Tissue Distribution; D014885:Waxes
chemical_list: D018076:DNA, Complementary; D004952:Esters; D005989:Glycerides; D008055:Lipids; D014885:Waxes; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase; C021802:2-acylglycerol O-acyltransferase; D051142:Retinol O-Fatty-Acyltransferase