Development of a murine prostatespecific 2-in-1 inducible bicistronic expression vector.
Transgenic research often suffers from the lack of strong tissue specific promoters and the lack of suitable antibodies for transgene detection. We have now constructed a novel prostate specific 2-in-1 Tetracycline-off (Tet-off), bicistronic expression vector, designated PbTetOIE, for transgenic research. The vector allows potent induction as well as inducible suppression of transgene expression in the prostate epithelial cells, and also allows the detection of transgene expression at the cellular level through the detection of the internal enhanced green fluorescent protein (EGFP) downstream of the transgene.
Young
Lei
L
Department of Medicine, University of Sydney, NSW, Australia. lyoung@student.usyd.edu.au
Dong
Qihan
Q
D000727:Androgen-Binding Protein / Q000235:genetics*; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011467:Prostate / Q000378:metabolism*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism; D013752:Tetracycline / Q000494:pharmacology; D014162:Transfection; D019076:Transgenes / Q000235:genetics*
D000727:Androgen-Binding Protein; D011993:Recombinant Fusion Proteins; C027519:probasin; D049452:Green Fluorescent Proteins; D009955:Ornithine Decarboxylase; D013752:Tetracycline