title: Pall eBDS: an enhanced bacterial detection system for screening platelet concentrates.
abstract: Bacterial contamination of blood components remains a significant problem in transfusion medicine. The Pall enhanced bacterial detection system (Pall eBDS) detects the presence of bacteria in leucodepleted platelet concentrates by measuring the reduction of oxygen in the sample, due to aerobic bacterial growth. Pooled platelet concentrates were spiked at 10 cfu mL(-1) with 10 organisms (one species per bag). Pall eBDS pouches were inoculated with the spiked platelet concentrates. After 24 and 30 h of incubation, the oxygen level was measured. A further set of pouches were taken from the inoculated platelet concentrates at 24 h. Incubation and reading intervals were as for the initial set of pouches. A sensitivity study was also performed comparing the Pall eBDS with the BacT/ALERT system. Spiking at 10 cfu mL(-1) and immediately sampling into Pall eBDS pouches resulted in 97.6 and 100% detection after an incubation period of 24 and 30 h, respectively. After 24 h of incubation of the spiked platelet concentrates and then sampling into Pall eBDS pouches, 99.1% detection was obtained after incubation for both 24 and 30 h. The sensitivity of the Pall eBDS and BacT/ALERT is similar and in the order of 1 cfu mL(-1). Implementation of either BacT/ALERT or Pall eBDS for routine screening of platelet concentrates has the potential to further increase the safety of the blood supply.
authors.affiliation: National Blood Service, Colindale, London, UK. carl.mcdonald@nbs.nhs.uk
authors.forename: C P
authors.initials: CP
authors.lastname: McDonald
authors.forename: S
authors.initials: S
authors.lastname: Pearce
authors.forename: K
authors.initials: K
authors.lastname: Wilkins
authors.forename: J
authors.initials: J
authors.lastname: Colvin
authors.forename: S
authors.initials: S
authors.lastname: Robbins
authors.forename: L
authors.initials: L
authors.lastname: Colley
authors.forename: J
authors.initials: J
authors.lastname: Taylor
authors.forename: J A J
authors.initials: JA
authors.lastname: Barbara
mesh_terms: D001420:Bacteria, Aerobic / Q000302:isolation & purification*; D001792:Blood Platelets / Q000382:microbiology*; D006801:Humans; D047589:Leukocyte Reduction Procedures; D008722:Methods; D010084:Oxidation-Reduction; D010100:Oxygen / Q000032:analysis / Q000378:metabolism; D017713:Platelet Transfusion / Q000592:standards*; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic; D010100:Oxygen