Pluchea lanceolata attenuates cadmium chloride induced oxidative stress and genotoxicity in Swiss albino mice.
Cadmium intoxication induces lipid peroxidation and causes oxidative damage to various tissues by altering antioxidant defence system enzymes. At 24 h after treatment with a single intraperitoneal dose of cadmium chloride (5 mg kg-1), Swiss albino mice showed a significant increase in the levels of malanodialdehyde and xanthine oxidase (P<0.001), and a concomitant depletion of renal glutathione, catalase (P<0.001) and other antioxidant enzymes. CdCl2 also led to a simultaneous increase in micronuclei formation (P<0.001) and chromosomal aberrations (P<0.05) in mouse bone marrow cells. Oral pre-treatment with Pluchea lanceolata extract at doses of 100 and 200 mg kg-1 for 7 consecutive days before CdCl2 intoxication caused a significant reduction in malanodialdehyde formation and xanthine oxidase activity (P<0.001). A significant restoration of the activity of antioxidant defence system enzymes such as catalase, glutathione peroxidase (P<0.05), glutathione-S-transferase and glutathione reductase (P<0.001) was observed. A significant dose-dependent decrease in chromosomal aberrations and micronuclei formation was also observed (P<0.05). The results indicate that pre-treatment with P. lanceolata attenuates cadmium chloride induced oxidative stress and genotoxicity by altering antioxidant enzymes and reducing chromatid breaks and micronuclei formation.
Jahangir
Tamanna
T
Section of Chemoprevention and Nutrition Toxicology, Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110062, India.
Khan
Tajdar Husain
TH
Prasad
Lakshmi
L
Sultana
Sarwat
S
D000284:Administration, Oral; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D019256:Cadmium Chloride / Q000008:administration & dosage / Q000009:adverse effects* / Q000037:antagonists & inhibitors*; D002374:Catalase / Q000037:antagonists & inhibitors / Q000187:drug effects; D002869:Chromosome Aberrations / Q000139:chemically induced / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry; D005978:Glutathione / Q000187:drug effects / Q000502:physiology; D005980:Glutathione Reductase / Q000187:drug effects / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008297:Male; D008515:Medicine, Ayurvedic; D051379:Mice; D015162:Micronucleus Tests / Q000379:methods; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry / Q000378:metabolism; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism
D010936:Plant Extracts; D000431:Ethanol; D002374:Catalase; D014969:Xanthine Oxidase; D005980:Glutathione Reductase; D005978:Glutathione; D019256:Cadmium Chloride