title: Detection and recovery of hydrolytic enzymes from spent compost of four mushroom species.
abstract: To evaluate the potential of using the enzymes from spent mushroom compost (SMC) as an industrial enzyme, the production of alpha-amylase, cellulase, beta-glucosidase, laccase, and xylanase was determined from the SMC of four edible mushroom species (Pleurotus ostreatus, Lentinula edodes, Flammulina velutipes and Hericium erinaceum). Among the tested SMC, the SMC of L. edodes showed the highest enzyme activity in alpha-amylase (229 nkat/g), cellulase (759 nkat/g) and beta-glucosidase (767 nkat/g) in 0.5% Triton X-100, and that of P. ostreatus showed the highest activity in laccase (1452 nkat/g) in phosphate-buffered 0.2% Triton X-100. The highest xylanase activity (119 nkat/g) was found in the SMC of F. velutipes.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
authors.forename: Han Gyu
authors.initials: HG
authors.lastname: Ko
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Park
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Kim
authors.forename: Hyuk Gu
authors.initials: HG
authors.lastname: Park
authors.forename: Won Mok
authors.initials: WM
authors.lastname: Park
mesh_terms: D001487:Basidiomycota / Q000145:classification / Q000201:enzymology* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002480:Cellulase / Q000302:isolation & purification / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000302:isolation & purification / Q000378:metabolism; D006867:Hydrolases / Q000302:isolation & purification* / Q000378:metabolism*; D042845:Laccase / Q000302:isolation & purification / Q000378:metabolism; D020076:Pleurotus / Q000201:enzymology; D020942:Shiitake Mushrooms / Q000201:enzymology; D000516:alpha-Amylases / Q000302:isolation & purification / Q000378:metabolism; D001617:beta-Glucosidase / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D042845:Laccase; D006867:Hydrolases; D000516:alpha-Amylases; D001617:beta-Glucosidase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases