Measurement of mast cell cytokine release by multiplex assay.
Mast cells are highly responsive cells that are capable of secreting a variety of inflammatory mediators, including histamine, heparin, serine proteases, leukotrienes, prostaglandins, and thromboxanes. Studies from several laboratories have demonstrated that mast cells have the capacity to produce a variety of cytokines in response to various stimuli. Characterization of the cytokine profiles in mast cells has routinely been determined by the performance of individual enzyme-linked immunosorbent assays. This process is expensive, time-consuming, and requires a great deal of material to characterize multiple cytokines. In this chapter, we describe a multiplex cytokine assay to detect 17 cytokines simultaneously in 50 microL of culture supernatant derived from stimulated human cord blood-derived mast cells.
Breuel
Kevin F
KF
Department of OB/GYN, Quillen College of Medicine, East Tennessee State University, Johnson City, TN, USA.
De Ponti
W Keith
WK
D001681:Biological Assay / Q000379:methods*; D016207:Cytokines / Q000032:analysis*; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D015759:Ionomycin / Q000378:metabolism; D007476:Ionophores / Q000378:metabolism; D008407:Mast Cells / Q000737:chemistry* / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism
D016207:Cytokines; D007476:Ionophores; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate