Dynamic range and reproducibility of hepatitis B virus (HBV) DNA detection and quantification by Cobas Taqman HBV, a real-time semiautomated assay.
The Cobas Taqman assay for hepatitis B virus (HBV) DNA showed linear detection over 7 logs for genotypes A to D. The coefficient of variation was 1.2% at > or =1,000 IU/ml and 22.0% at 10 IU/ml. In 97 clinical samples, the log HBV DNA/ml differed by 0.11 between Cobas Amplicor and Cobas Taqman (r2 = 0.97).
Lindh
Magnus
M
Department of Clinical Virology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden. magnus.lindh@microbio.gu.se
Hannoun
Charles
C
D001331:Automation; D004279:DNA, Viral / Q000032:analysis*; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D016031:Liver Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D014766:Viremia / Q000821:virology
D004279:DNA, Viral