title: Comparison of the antimicrobial adhesion potential of human body fluid glycoconjugates using fucose-binding lectin (PA-IIL) of Pseudomonas aeruginosa and Ulex europaeus lectin (UEA-I).
abstract: Pseudomonas aeruginosa produces a fucose-binding lectin (PA-IIL) which strongly binds to human cells. This lectin was shown to be highly sensitive to inhibition by fucose-bearing human milk glycoproteins. Since the glycans of these glycoproteins mimic human cell receptors, they may function as decoys in blocking lectin-dependent pathogen adhesion to the host cells. Human saliva and seminal fluid also contain such compounds, and body fluids of individuals who are "secretors" express additional fucosylated (alpha 1,2) residues. The latter are selectively detected by Ulex europaeus lectin UEA-I. The aim of the present research was to compare the PA-IIL and UEA-I interactions with human salivas and seminal fluids of "secretors" and "nonsecretors" with those obtained with the respective milks. Using hemagglutination inhibition and Western blot analyses, we showed that PA-IIL interactions with the saliva and seminal fluid glycoproteins were somewhat weaker than those obtained with the milk and that "nonsecretor" body fluids were not less efficient than those of "secretors" in PA-IIL blocking. UEA-I, which interacted only with the "secretors" glycoproteins, was most sensitive to those of the seminal fluids.
authors.affiliation: Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel.
authors.forename: Batia
authors.initials: B
authors.lastname: Lerrer
authors.forename: Efrat
authors.initials: E
authors.lastname: Lesman-Movshovich
authors.forename: Nechama
authors.initials: N
authors.lastname: Gilboa-Garber
mesh_terms: D001422:Bacterial Adhesion; D015153:Blotting, Western; D005260:Female; D006001:Glycoconjugates / Q000032:analysis / Q000378:metabolism*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008895:Milk, Human / Q000378:metabolism*; D037121:Plant Lectins / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D012463:Saliva / Q000378:metabolism*; D012661:Semen / Q000378:metabolism*
chemical_list: D006001:Glycoconjugates; D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; C027112:fucose-binding lectin