Constitutive alpha-amylase producing mutant and recombinant haploid strains of thermophilic fungus Thermomyces lanuginosus.
Morphological, developmental and antimetabolite-resistant mutants of T. lanuginosus were characterized and used for screening with the aim to develop constitutive alpha-amylase-hyperproducing strains. The protoplast fusion of two spontaneous mutants of T. lanuginosus, characterized as asporulating and resistant to 2-deoxy-D-glucose (2DG), resulted in sporulating, 2DG sensitive heterokaryotic fusants. A recombinant haploid strain F64fB developed there from produced alpha-amylase constitutively in glucose-containing medium. Constitutive alpha-amylase-hyperproducing mutant (III8) obtained after cyclic mutagenesis and screening yielded approximately 20 fold more alpha-amylase in a glycerol-containing medium than the wild strain.
Chadha
B S
BS
Department of Microbiology, Guru Nanak Dev University, Amritsar, 143 005 India.
Rubinder
K
K
Saini
H S
HS
D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001709:Biotechnology / Q000379:methods*; D006238:Haploidy; D006358:Hot Temperature; D009024:Morphogenesis; D016296:Mutagenesis; D009154:Mutation; D011995:Recombination, Genetic; D013172:Spores, Fungal; D000516:alpha-Amylases / Q000096:biosynthesis*
D000516:alpha-Amylases