Transthyretin-related proteins function to facilitate the hydrolysis of 5-hydroxyisourate, the end product of the uricase reaction.
Purine catabolic pathway in Bacillus subtilis is consisted of more than 14 genes. Among these genes, pucL and pucM are required for uricase activity. While PucL is known to encode the uricase itself, the function of PucM is still unclear although this protein is also indispensable for uric acid decomposition. Here, we provide evidence that PucM, a transthyretin-related protein, functions to facilitate the hydrolysis of 5-hydroxyisourate, the end product of the uricase reaction. Based on these results, we propose that transthyretin-related proteins present in diverse organisms are not functionally related to transthyretin but actually function as a hydroxyisourate hydrolase.
Lee
Youra
Y
Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Oun-dong, Yuseong-gu, Daejeon 305-333, South Korea.
Lee
Do Hee
DH
Kho
Chang Won
CW
Lee
Ah Young
AY
Jang
Mi
M
Cho
Sayeon
S
Lee
Choong Hwan
CH
Lee
Jong Suk
JS
Myung
Pyung Keun
PK
Park
Byoung Chul
BC
Park
Sung Goo
SG
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011228:Prealbumin / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014503:Urate Oxidase / Q000378:metabolism*; D014527:Uric Acid / Q000737:chemistry* / Q000378:metabolism
D001426:Bacterial Proteins; D011228:Prealbumin; D014527:Uric Acid; D014503:Urate Oxidase