title: Peptide mass mapping of acetylated isoforms of histone H4 from mouse lymphosarcoma cells treated with histone deacetylase (HDACs) inhibitors.
abstract: The acetylated isoforms of histone H4 from mouse lymphosarcoma cells treated with HDAC inhibitors trichostatin A (TSA) and depsipeptide (DDP) were separated by acetic acid urea-polyacrylamide gel electrophoresis (AU-PAGE), in-gel digested, and analyzed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS). The acetylation pattern of histone H4 in mouse lymphosarcoma cells induced by TSA was established in which acetylation initially occurred at K16 followed by K12 and then K8 and/or K5. An identical order of acetylation was found for cells treated with DDP.
authors.affiliation: Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Ren
authors.forename: Liwen
authors.initials: L
authors.lastname: Zhang
authors.forename: Michael A
authors.initials: MA
authors.lastname: Freitas
authors.forename: Kalpana
authors.initials: K
authors.lastname: Ghoshal
authors.forename: Mark R
authors.initials: MR
authors.lastname: Parthun
authors.forename: Samson T
authors.initials: ST
authors.lastname: Jacob
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D045744:Cell Line, Tumor; D047630:Depsipeptides / Q000008:administration & dosage*; D056572:Histone Deacetylase Inhibitors; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000008:administration & dosage*; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D047630:Depsipeptides; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; D020033:Protein Isoforms; C012589:trichostatin A