title: ERM is required for transcriptional control of the spermatogonial stem cell niche.
abstract: Division of spermatogonial stem cells produces daughter cells that either maintain their stem cell identity or undergo differentiation to form mature sperm. The Sertoli cell, the only somatic cell within seminiferous tubules, provides the stem cell niche through physical support and expression of surface proteins and soluble factors. Here we show that the Ets related molecule (ERM) is expressed exclusively within Sertoli cells in the testis and is required for spermatogonial stem cell self-renewal. Mice with targeted disruption of ERM have a loss of maintenance of spermatogonial stem cell self-renewal without a block in normal spermatogenic differentiation and thus have progressive germ-cell depletion and a Sertoli-cell-only syndrome. Microarray analysis of primary Sertoli cells from ERM-deficient mice showed alterations in secreted factors known to regulate the haematopoietic stem cell niche. These results identify a new function for the Ets family transcription factors in spermatogenesis and provide an example of transcriptional control of a vertebrate stem cell niche.
authors.affiliation: Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Wenjun
authors.initials: W
authors.lastname: Ouyang
authors.forename: Vadim
authors.initials: V
authors.lastname: Grigura
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Kay
authors.initials: K
authors.lastname: Carnes
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Lim
authors.forename: Guang-Quan
authors.initials: GQ
authors.lastname: Zhao
authors.forename: Silvia
authors.initials: S
authors.lastname: Arber
authors.forename: Natasza
authors.initials: N
authors.lastname: Kurpios
authors.forename: Theresa L
authors.initials: TL
authors.lastname: Murphy
authors.forename: Alec M
authors.initials: AM
authors.lastname: Cheng
authors.forename: John A
authors.initials: JA
authors.lastname: Hassell
authors.forename: Varadaraj
authors.initials: V
authors.lastname: Chandrashekar
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Hofmann
authors.forename: Rex A
authors.initials: RA
authors.lastname: Hess
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Murphy
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics*; D013093:Spermatogonia / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; C513242:Etv5 protein, mouse; D014157:Transcription Factors