title: A transposon-based activation-tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations.
abstract: A population of 9471 stable activation-tagged lines was generated by transposable element mediated activation tagging mutagenesis in Arabidopsis (TAMARA) using the maize En/Spm transposon system. Based on DNA gel blot and flanking sequence analysis, this population contains approximately 6000 independent transposon insertions. A greenhouse-based screen identified six dominant or semi-dominant activation tagged mutants with obvious developmental alterations, among these a new pistillata mutant allele. In addition, a subset of 1500 lines was screened by a HPLC based high-throughput method for dominant activation tagged mutants with enhanced contents of phenolic compounds. One dominant activation tagged mutant (hpc1-1D) was isolated showing accumulation of a particular compound due to the upregulation of an R2R3-MYB transcription factor.
authors.affiliation: Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50931 Köln, Germany. Anja.Schneider@uni-koeln.de
authors.forename: Anja
authors.initials: A
authors.lastname: Schneider
authors.forename: Thomas
authors.initials: T
authors.lastname: Kirch
authors.forename: Tamara
authors.initials: T
authors.lastname: Gigolashvili
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Mock
authors.forename: Uwe
authors.initials: U
authors.lastname: Sonnewald
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Simon
authors.forename: Ulf-Ingo
authors.initials: UI
authors.lastname: Flügge
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Werr
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism; D032461:Chromosomes, Plant; D004252:DNA Mutational Analysis; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016415:Sequence Alignment; D016324:Sequence Tagged Sites
chemical_list: D004251:DNA Transposable Elements; D005819:Genetic Markers