Differential activation of a latent polyphenol oxidase mediated by sodium dodecyl sulfate.
A kinetic study of the activity of soluble and membrane-bound latent polyphenol oxidase (PPO) extracted from beet root (Beta vulgaris) was carried out. For the first time, two types of behavior (hyperbolic and sigmoid) are reported in the same enzyme for PPO activation by the surfactant sodium dodecyl sulfate (SDS), depending on substrate nature. A kinetic model based on cooperative systems is developed to describe the activation effect of SDS, enabling the determination of the number of surfactant molecules binding to the enzyme in the activation process. The results indicate that the active site of the enzyme is not affected by SDS and that a stepwise conformational change favors the access of hydrophobic substrates compared to hydrophilic ones. Differential activation of PPO mediated by SDS may be of relevance in the control of PPO activity since the enzyme is able to express activity toward a specific substrate while remaining latent to others.
Gandía-Herrero
Fernando
F
Departamento de Bioquímica y Biología Molecular A, Unidad Docente de Biología, Facultad de Veterinaria, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain.
Jiménez-Atiénzar
Mercedes
M
Cabanes
Juana
J
García-Carmona
Francisco
F
Escribano
Josefa
J
D027461:Beta vulgaris / Q000201:enzymology*; D004156:Catechol Oxidase / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D018517:Plant Roots / Q000201:enzymology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*
D012967:Sodium Dodecyl Sulfate; D004156:Catechol Oxidase