White shrimp (Litopenaeus vannamei) recombinant lysozyme has antibacterial activity against Gram negative bacteria: Vibrio alginolyticus, Vibrio parahemolyticus and Vibrio cholerae.
C-type lysozyme has been described as an antibacterial component of the shrimp innate defence system. We determined quantitatively the antibacterial activity of white shrimp (Litopenaeus vannamei) recombinant lysozyme against three Gram negative bacteria: Vibrio alginolyticus, Vibrio parahemolyticus and Vibrio cholerae, using a turbidimetric assay with live bacteria and differential bacterial viable count after interaction with the protein. In conclusion, the antibacterial activity of recombinant shrimp lysozyme against Vibrio sp. is at least equal to the values against the Gram positive M. luteus and more active against the shrimp pathogens V. alginolyticus and V. parahemolyticus.
de-la-Re-Vega
Enrique
E
Aquatic Molecular Biology Laboratory, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Sonora 83000, Mexico.
García-Galaz
Alfonso
A
Díaz-Cinco
Martha E
ME
Sotelo-Mundo
Rogerio R
RR
D000818:Animals; D009113:Muramidase / Q000276:immunology* / Q000494:pharmacology; D009391:Nephelometry and Turbidimetry; D033561:Penaeidae / Q000276:immunology* / Q000382:microbiology*; D011994:Recombinant Proteins / Q000276:immunology* / Q000494:pharmacology; D013997:Time Factors; D014733:Vibrio / Q000187:drug effects* / Q000276:immunology
D011994:Recombinant Proteins; D009113:Muramidase