In situ stable isotope probing of methanogenic archaea in the rice rhizosphere.
Microorganisms living in anoxic rice soils contribute 10 to 25% of global methane emissions. The most important carbon source for CH4 production is plant-derived carbon that enters soil as root exudates and debris. Pulse labeling of rice plants with 13CO2 resulted in incorporation of 13C into the ribosomal RNA of Rice Cluster I Archaea in the soil, indicating that this archaeal group plays a key role in CH4 production from plant-derived carbon. This group of microorganisms has not yet been isolated but appears to be of global environmental importance.
Lu
Yahai
Y
College of Resources and Environmental Sciences, China Agricultural University, Beijing 100094, China.
Conrad
Ralf
R
D001105:Archaea / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes / Q000378:metabolism*; D003001:Cloning, Molecular; D017753:Ecosystem; D006859:Hydrogen / Q000378:metabolism; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism / Q000382:microbiology*; D010788:Photosynthesis; D010802:Phylogeny; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D019642:RNA, Archaeal / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D012988:Soil Microbiology
D002247:Carbon Isotopes; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D002245:Carbon Dioxide; D006859:Hydrogen; D008697:Methane