[Combination effects of vitamin A and zinc on human's PMBC DNA damage in vitro].
OBJECTIVE
To study the combination effects of vitamin A (Vit. A) and zinc on human's PBMC DNA damage in vitro. Methods The PBMC from healthy volunteer was purification and cultured in the basic culture medium with Vit.A of 10(-4), 10(-5) and 10(-6) mol/L, or/and with Zn of 10(-6), 10(-5) 10(-4) and 10(-3) mol/L. The basic culture medium with K2Cr2O7 of 1 mmol/L was the male control. The damage of PBMC DNA was respectively examined by SCGE and the image analyzer measured the comet tail rate and length. The apoptosis of each group PBMC was detected by FCM.


RESULTS
The overdose supplement of zinc and Vit. A can damage the PBMC DNA in vitro culture (P < 0.01), while the suitable supplement doesn't find. When the Vit. A supplement has been excessive, giving suitable zinc could repair the damage of DNA, but would expand the injured cell. While when the zinc supplement has been excessive, giving suitable Vit. A could protect the healthy cells, but would aggravate the damage degree of injured cell.


CONCLUSIONS
In vitro culture, the overdose Vit. A and zinc supplement could damage the human's PBMC DNA significantly. This need more researches to provide.
Li
Ting-Xin
TX
West China School of Public Health,Sichuan University, Chengdu 610041, China.
Li
Yun
Y
Lu
Zi-Long
ZL
D002478:Cells, Cultured; D004249:DNA Damage; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects*; D014801:Vitamin A / Q000008:administration & dosage / Q000009:adverse effects*; D015032:Zinc / Q000008:administration & dosage / Q000009:adverse effects*
D014801:Vitamin A; D015032:Zinc