Resveratrol inhibits type II phosphatidylinositol 4-kinase: a key component in pathways of phosphoinositide turn over.
Resveratrol has anti-inflammatory, cardio protective and cancer chemopreventive properties. The molecular targets for resveratrol in early signaling cascades are not well understood. Resveratrol inhibits type II PtdIns 4-kinase but not PtdIns 3-kinase activity in vitro. Resveratrol directly binds to the enzyme with a Kd of 7.2 microM. Kinetic studies show that resveratrol competes with PtdIns binding. Inhibition of PtdIns 4-kinase activity by resveratrol/phenylarsine oxide reduces Jurkat cell adhesion to matrigel/fibronectin coated surfaces, suggesting a role for type II PtdIns 4-kinase in lymphocyte infiltration to the sites of inflammation.
Srivastava
Rupa
R
Biotechnology group, School of Bioscience and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Ratheesh
Aparna
A
Gude
Rajiv K
RK
Rao
K V K
KV
Panda
Dulal
D
Subrahmanyam
Gosukonda
G
D019870:1-Phosphatidylinositol 4-Kinase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D003001:Cloning, Molecular; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D010716:Phosphatidylinositols / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D010716:Phosphatidylinositols; D013267:Stilbenes; D019870:1-Phosphatidylinositol 4-Kinase; D000077185:Resveratrol