Functional analysis of organic cation transporter 3 expressed in human placenta.
The aim of this study is to investigate the placental transport mechanism of cationic compounds by comparison of the uptake of an organic cation into human placental basal membrane vesicles (BLMVs) with that into organic cation transporter 3 (OCT3)-expressing cells. Reverse transcription-polymerase chain reaction analysis demonstrated that OCT3 is the only OCT isoform expressed in the human placenta. The function of OCT3 was investigated by measuring the uptake of 1-methyl-4-phenylpyridinium (MPP(+)) into human embryonic kidney (HEK)293 cells stably expressing OCT3 (HEK/OCT3 cells). The OCT3-mediated uptake of MPP(+) was sodium- and chloride-independent and saturable, with a Michaelis constant (K(m)) of 82.5 microM. The OCT3-mediated uptake was inhibited by various cationic drugs in a concentration-dependent manner but not by anionic compounds, such as p-aminohippuric acid and captopril, or a zwitterion, carnitine. Western blotting analysis of membrane vesicles prepared from human term placenta revealed that OCT3 is expressed only in BLMVs but not in microvillous membrane vesicles. The uptake of MPP(+) into BLMVs was membrane potential-dependent and saturable, with a K(m) value of 51.8 muM, which is similar to that in HEK293/OCT3 cells. The inhibitory spectrum of various compounds on MPP(+) uptake by BLMVs was also similar to that in HEK293/OCT3 cells. These results suggest that OCT3 is expressed on the basal membrane of human trophoblast cells and plays an important role in the placental transport of cationic compounds.
Sata
Ryoko
R
Department of Medico-Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Ohtani
Hisakazu
H
Tsujimoto
Masayuki
M
Murakami
Hideyasu
H
Koyabu
Noriko
N
Nakamura
Takanori
T
Uchiumi
Takeshi
T
Kuwano
Michihiko
M
Nagata
Hideaki
H
Tsukimori
Kiyomi
K
Nakano
Hitoo
H
Sawada
Yasufumi
Y
D000328:Adult; D015153:Blotting, Western; D002412:Cations / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002824:Chorionic Villi / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D027701:Organic Cation Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000099254:Organic Cation Transporter 3
D002412:Cations; D027701:Organic Cation Transport Proteins; D012333:RNA, Messenger; D000099254:Organic Cation Transporter 3