Applications of the double-barreled data in whole-genome shotgun sequence assembly and analysis.
Double-barreled (DB) data have been widely used for the assembly of large genomes. Based on the experience of building the whole-genome working draft of Oryza sativa L. ssp. Indica, we present here the prevailing and improved uses of DB data in the assembly procedure and report on novel applications during the following data-mining processes such as acquiring precise insert fragment information of each clone across the genome, and a new kind of low-cost whole-genome microarray. With the increasing number of organisms being sequenced, we believe that DB data will play an important role both in other assembly procedures and in future genomic studies.
Han
Yujun
Y
College of Life Sciences, Peking University, Beijing 100871, China.
Ni
Peixiang
P
Lü
Hong
H
Ye
Jia
J
Hu
Jianfei
J
Chen
Chen
C
Huang
Xiangang
X
Cong
Lijuan
L
Li
Guangyuan
G
Wang
Jing
J
Gu
Xiaocheng
X
Yu
Jun
J
Li
Songgang
S
D003001:Cloning, Molecular / Q000379:methods*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D015723:Gene Library; D018745:Genome, Plant; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*
D018744:DNA, Plant