title: Accumulation of 23 kDa lipocalin during brain development and injury in Hyphantria cunea.
abstract: The cDNA corresponding to a novel lipocalin was identified from the fall webworm, Hyphantria cunea. This lipocalin cDNA encodes a 194 residue protein with a calculated molecular mass of 23 kDa. Sequence analyses revealed that the 23 kDa lipocalin cDNA is most similar to Drosophila lazarillo, human apolipoprotein D, and Bombyrin. Northern blot analyses showed that 23 kDa lipocalin transcript is expressed in the whole body only in 4- and 6-day-old pupae. By Western blot analysis it was confirmed that 23 kDa lipocalin is mainly accumulated in brain and subesophageal ganglion, though it is detected in a small amount in fat body and epidermis of Hyphantria cunea. The accumulation of 23 kDa lipocalin in brain tissue was upregulated in response to injury. The putative function of 23 kDa lipocalin in brain is discussed.
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Hong Ja
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Je
authors.forename: Hyang Mi
authors.initials: HM
authors.lastname: Cheon
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kong
authors.forename: JikHyun
authors.initials: J
authors.lastname: Han
authors.forename: Chi Young
authors.initials: CY
authors.lastname: Yun
authors.forename: Yeon Su
authors.initials: YS
authors.lastname: Han
authors.forename: In Hee
authors.initials: IH
authors.lastname: Lee
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Kang
authors.forename: Sook Jae
authors.initials: SJ
authors.lastname: Seo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D029721:Drosophila Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009036:Moths / Q000254:growth & development*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D011679:Pupa; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins