Expression and modulation of LL-37 in normal human keratinocytes, HaCaT cells, and inflammatory skin diseases.
Defensins and cathelicidins (LL-37) are major antimicrobial peptides (AMPs) of the innate immune system of the human skin. In normal non-inflamed skin these peptides are negligible, but their expression can be markedly increased in inflammatory skin disease such as psoriasis. We designed this study to identify the expressions of LL-37 in normal human keratinocyte (NHK) and HaCaT cells after exposure to stimulants and to investigate difference of LL-37 expression accompanied with cell differentiation status, and come to understand difference of susceptibility to infection in atopic dermatitis and psoriasis. Expressions of LL-37 in NHKs and HaCaT cells were evaluated by using RT-PCR, Western blotting, and immunohistochemical (IHC) staining at 6, 12, and 24 hr post stimulation after exposure to Ultraviolet B irradiation and lipopolysaccharide. And expression of LL-37 in skin biopsy specimens from patients with atopic dermatitis and psoriasis was determined by immunohistochemical analysis. In time-sequential analyses of LL-37 expression revealed that LL-37 was expressed in NHKs, but not in HaCaT cells. IHC analysis confirmed the presence of abundant LL-37 in the epidermis of psoriasis. Therefore we deduced that expression of LL-37 is affected by UV irradiation, bacterial infection, and status of cell differentiation.
Kim
Ji Eun
JE
Department of Dermatology, College of Medicine, Chung Ang University, Korea.
Kim
Beom Joon
BJ
Jeong
Mi Sook
MS
Seo
Seong Jun
SJ
Kim
Myeung Nam
MN
Hong
Chang Kwun
CK
Ro
Byung In
BI
D023181:Antimicrobial Cationic Peptides / Q000032:analysis / Q000235:genetics*; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D023082:Defensins / Q000032:analysis / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology / Q000378:metabolism; D012871:Skin Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D054804:Cathelicidins
D023181:Antimicrobial Cationic Peptides; D023082:Defensins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D054804:Cathelicidins