[Inhibitory effect of sodium butyrate on 1,2-dimethylhydrazine-induced tumorigenesis of colorectal cancer in mice].
OBJECTIVE
In vitro studies proved that sodium butyrate (NaB) could stimulate cell differentiation, inhibit cell proliferation, and induce cell apoptosis in various tumors. This study was to evaluate the preventive effects of coloclysis of NaB on 1,2-dimethylhydrazine (DMH)-induced tumorigenesis of colorectal cancer in mice.


METHODS
The mice of Kunming species were divided into 5 groups and received relevant treatments: DMH alone group (with subcutaneous injection of 30 mg/kg of DMH weekly for 11 weeks), control (normal saline, NS) group, DMH plus low dose of NaB (1.25 mol/kg, 24-week coloclysis) group, DMH plus high dose of NaB (2.5 mol/kg, 24-week coloclysis) group, and high dose of NaB group. The mice were killed in batches at the 12th, 18th, and 24th weeks of carcinoma induction separately. The incidence of colorectal tumor in each group was observed. Meanwhile, the general condition, body weight increasing, liver and renal functions, and pathologic changes of liver, kidney, lungs, and pancreas of the mice in DMH plus high dose of NaB group were also observed.


RESULTS
No tumor was observed at the 12th week in each group. At the 18th week, the tumor incidence was significantly higher in DMH alone group and DMH plus low dose of NaB group than in DMH plus high dose of NaB group (58.3%, and 25.0% vs. 0, P < 0.01). At the 24th week, the tumor incidence was 95.0% in DMH alone group, 45.0% in DMH plus low dose of NaB group, and 15.0% in DMH plus high dose of NaB group; the differences among the 3 groups were significant (P=0.01, P < 0.01). No tumor was observed in control group and high dose of NaB group. There was no difference in the general condition, body weight increasing, and liver and renal functions of the mice between control group and high dose of NaB group (P > 0.05); no pathologic changes in liver, kidney, lungs, and pancreas were observed in the mice in high dose of NaB group.


CONCLUSIONS
NaB could inhibit DMH-induced tumorigenesis of colorectal cancer in mice with no obvious toxicity.
Liu
Cheng-Xia
CX
Department of Digestive Disease, Qilu Hospital, Shandong University, Jinan, Shandong, P. R. China. bzlcx@yahoo.com.cn
Zhang
Shang-Zhong
SZ
Zhang
Xiao-Wei
XW
Geng
Xiu-Lan
XL
Li
Tie-Jun
TJ
Huang
Li-Hua
LH
Wang
Bing
B
D019813:1,2-Dimethylhydrazine; D000230:Adenocarcinoma / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D000236:Adenoma / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D000818:Animals; D002087:Butyrates / Q000008:administration & dosage / Q000627:therapeutic use* / Q000633:toxicity; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007413:Intestinal Mucosa / Q000473:pathology; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D051379:Mice
D002087:Butyrates; D019813:1,2-Dimethylhydrazine