Genetic alteration of anxiety and stress-like behavior in mice lacking CaMKIV.
Calcium-calmodulin-dependent protein kinase IV (CaMKIV) phosphorylates the major transcription factor cyclic AMP-response element binding protein (CREB), which plays a role in emotional behavior. Here, CaMKIV knockout mice (CaMKIV(-/-)) were tested in a battery of stress and anxiety-related behavioral tests, to determine if CaMKIV plays a role in emotional behavior. CaMKIV(-/-) exhibited a decrease in anxiety-like behavior in both the elevated plus maze and dark-light emergence tests when compared to wild-type mice. Both the acoustic startle response and prepulse inhibition of startle were decreased with the deletion of CaMKIV. In addition, CaMKIV(-/-) mice displayed a lack of stress-induced analgesia following restraint or cold swim stress. Our results demonstrate a key role for CaMKIV in anxiety and stress-related behavior.
Shum
Fanny W F
FW
Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, M5S 1A8, Canada. fanny.shum@utoronto.ca
Ko
Shanelle W
SW
Lee
Yong-Seok
YS
Kaang
Bong-Kiun
BK
Zhuo
Min
M
D000698:Analgesia; D000818:Animals; D001007:Anxiety / Q000150:complications / Q000201:enzymology* / Q000235:genetics*; D001522:Behavior, Animal; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4 / Q000172:deficiency* / Q000378:metabolism; D003624:Darkness; D005106:Exploratory Behavior; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010121:Oxytocin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013216:Reflex, Startle / Q000235:genetics; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000150:complications / Q000201:enzymology* / Q000235:genetics*; D013550:Swimming
D012333:RNA, Messenger; D010121:Oxytocin; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4