NR1, NR2A and NR2C subunits expression after cervical spinal cord transplant and section in dogs.
This paper served to evaluate the expression levels of subunits NR1, NR2A and NR2C which are implicated in neuronal plasticity events. A 50% (right half) 4 mm longitudinal resection of the spinal cord was done at the C5-C6 level with preservation of the anterior spinal artery. This was effected in a dog model after either a homologous transplant or a pure spinal cord section. In this study we used two groups of dogs with four individuals each, as well as a control group. The transplant group (n=4) was analyzed at days 3 and 28 post surgery. The section group (n=4) was also analyzed at days 3 and 28 post op. All three groups (transplant, section and control) were evaluated as to the subunit expression in each of the segments corresponding to the transplanted or sectioned sites, the site contralateral to the transplanted or sectioned sites at levels half a centimeter both proximal and distal to the site of transplant and section. The results showed a variety of changes in each of the subunits depending on the group, the segment and the time of evaluation (acute versus chronic). This could be closely related to mechanisms which participate in regeneration and functional recuperation.
Alatorre
W E Bitar
WE
Lab. Neurobiol. Cel. y Molec. División de Neurociencias, C.I.B.O-IMSS, Mexico. dremiliobitar@prodigy.net.mx
Flores Soto
M E
ME
Beas Zarate
C
C
D000818:Animals; D004285:Dogs; D008297:Male; D009473:Neuronal Plasticity / Q000502:physiology; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D016194:Receptors, N-Methyl-D-Aspartate / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013116:Spinal Cord / Q000378:metabolism* / Q000637:transplantation*; D013119:Spinal Cord Injuries / Q000378:metabolism*
C409466:NR1 NMDA receptor; C120997:NR2A NMDA receptor; C432823:NR2C NMDA receptor; D016194:Receptors, N-Methyl-D-Aspartate; D012313:RNA