title: Establishment of novel embryonic stem cell lines derived from the common marmoset (Callithrix jacchus).
abstract: The successful establishment of human embryonic stem cell (hESC) lines has inaugurated a new era in regenerative medicine by facilitating the transplantation of differentiated ESCs to specific organs. However, problems with the safety and efficacy of hESC therapy in vivo remain to be resolved. Preclinical studies using animal model systems, including nonhuman primates, are essential to evaluate the safety and efficacy of hESC therapies. Previously, we demonstrated that common marmosets are suitable laboratory animal models for preclinical studies of hematopoietic stem cell therapies. As this animal model is also applicable to preclinical trials of ESC therapies, we have established novel common marmoset ESC (CMESC) lines. To obtain marmoset embryos, we developed a new embryo collection system, in which blastocysts can be obtained every 3 weeks from each marmoset pair. The inner cell mass was isolated by immunosurgery and plated on a mouse embryonic feeder layer. Some of the CMESC lines were cultured continuously for more than 1 year. These CMESC lines showed alkaline phosphatase activity and expressed stage-specific embryonic antigen (SSEA)-3, SSEA-4, TRA-1-60, and TRA-1-81. On the other hand, SSEA-1 was not detected. Furthermore, our novel CMESCs are pluripotent, as evidenced by in vivo teratoma formation in immunodeficient mice and in vitro differentiation experiments. Our established CMESC lines and the common marmoset provide an excellent experimental model system for understanding differentiation mechanisms, as well as the development of regenerative therapies using hESCs.
authors.affiliation: Division of Laboratory Animal Science, Central Institute for Experimental Animals, Kanagawa, and Department of Urology, Urayasu Hospital, Juntendo University, Chiba, Japan.
authors.forename: Erika
authors.initials: E
authors.lastname: Sasaki
authors.forename: Kisaburo
authors.initials: K
authors.lastname: Hanazawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Kurita
authors.forename: Akira
authors.initials: A
authors.lastname: Akatsuka
authors.forename: Takahito
authors.initials: T
authors.lastname: Yoshizaki
authors.forename: Hajime
authors.initials: H
authors.lastname: Ishii
authors.forename: Yoshikuni
authors.initials: Y
authors.lastname: Tanioka
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suemizu
authors.forename: Ayako
authors.initials: A
authors.lastname: Sugawara
authors.forename: Norikazu
authors.initials: N
authors.lastname: Tamaoki
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Izawa
authors.forename: Yukoh
authors.initials: Y
authors.lastname: Nakazaki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Hamada
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Suemori
authors.forename: Shigetaka
authors.initials: S
authors.lastname: Asano
authors.forename: Norio
authors.initials: N
authors.lastname: Nakatsuji
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Kenzaburo
authors.initials: K
authors.lastname: Tani
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002144:Callithrix; D002454:Cell Differentiation; D002460:Cell Line; D004195:Disease Models, Animal; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D008297:Male; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*