[The study of interaction between the osteoblast and bio-derived material by detecting the gene expression].
OBJECTIVE
To study the gene expressions of human osteoblasts during the construction of tissue engineered bone with the bio-derived material.


METHODS
The fetal osteoblasts were used to construct tissue engineered bone with the bio-derived material and then were cultured 2, 4, 6, 8 and 10 days in vitro. Real-time PCR analysis indicated that Cbfa 1, Osterix, Collagen type I, osteocalcin (OC) and Integrin alpha5 and beta1 were present in osteoblasts with bio-derived materials. Results The change of Cbfa1 was consistent with the change of Osterix. On 2nd day and 8th day, the expression of Osterix in experimental group was higher than that in control group, P<0.05. Collagen type I's change was consistent with change of OC expression, and its expression was higher in experimental group than that in control group on 2nd, 4th, 6th and 8th day. The Integrin expression was high all along. Conclusion The important genes can be expressed normally by integrating osteoblasts with bio-derived scaffolds. As skeleton tissue engineering scaffold, the bio-derived bone is conducive to keep the osteoblast's phenotype and differentiation with osteo-conductive ability. The osteoblast can enter proliferation stage favorably and the scaffold materials exert no effects on it. Bio-derived bone can also supply more space for cells to proliferate. The bio-derived materials promote osteoblasts adhesion.
Qi
Jie
J
State Key Laboratory of Biotherapy,West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P. R. China.
Yang
Zhiming
Z
Xie
Huiqi
H
D000328:Adult; D018786:Bone Substitutes; D001842:Bone and Bones; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D005333:Fetus; D015870:Gene Expression; D006801:Humans; D039081:Integrin alpha5beta1 / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D015675:Osteocalcin / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D014157:Transcription Factors / Q000235:genetics
D018786:Bone Substitutes; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D039081:Integrin alpha5beta1; D014157:Transcription Factors; D015675:Osteocalcin