Induction of glutathione S-transferase and glutathione by toxic compounds and elicitors in reed canary grass.
Treatment of read canary grass leaves with phenol, 4-chlorophenol, naphthalic anhydride and phenylethylisothiocyanate increased glutathione S-transferase activity by 1.4-2.4-fold (control 17 U g(-1) DW). Benzothiadiazole, beta-aminobutyric acid and salicylic acid increased activity by 1.3-1.8-fold. Total glutathione pool was increased by the toxic compounds by 1.2-2-fold and by the elicitors 1.4-1.6-fold (control 593 nmol g(-1) DW). Unlike the other compounds, benzothiadiazole and salicylic acid did not decrease the redox state. Benzothiadiazole acted synergistically with chlorophenol on glutathione S-transferase and glutathione levels and counteracted the decrease in redox state caused by the xenobiotic. Reed canary grass thus has a strong potential to neutralize toxic compounds, which may be further enhanced by elicitors.
Urbanek
H
H
Department of Plant Physiology and Biochemistry, University of Łódź, 90-237 Łódź, Poland. henkurb@biol.uni.lodz.pl
Majorowicz
H
H
Zalewski
M
M
Saniewski
M
M
D000613:Aminobutyrates / Q000494:pharmacology; D002733:Chlorophenols / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D017879:Isothiocyanates / Q000494:pharmacology; D009281:Naphthalenes / Q000494:pharmacology; D009930:Organic Chemicals / Q000494:pharmacology*; D031783:Phalaris / Q000187:drug effects* / Q000378:metabolism; D019800:Phenol / Q000494:pharmacology; D020156:Salicylic Acid / Q000494:pharmacology; D013830:Thiadiazoles / Q000494:pharmacology
D000613:Aminobutyrates; D002733:Chlorophenols; D017879:Isothiocyanates; D009281:Naphthalenes; D009930:Organic Chemicals; D013830:Thiadiazoles; C015700:benzo-1,2,3-thiadiazole; D019800:Phenol; C047667:3-aminobutyric acid; C058305:phenethyl isothiocyanate; D005982:Glutathione Transferase; D005978:Glutathione; D020156:Salicylic Acid