The mRNA expression differences of RNA editing enzymes in differentiated and undifferentiated NG108-15 cells.
The alteration of the editing pattern of serotonin 2C receptor (5-HT2CR) mRNA has been reported during cell differentiation. Since editing of 5-HT2CR mRNA is regulated by adenosine deaminases acting on RNA (ADARs), it is of interest to investigate if the expression of these enzymes changes during cell differentiation. The level of ADAR1 mRNA in NG108-15 cells was decreased by cell differentiation. These results suggest that the decrease of ADAR1 expression during cell differentiation may play an important role in the differentiation-induced alteration of the 5-HT2CR editing pattern in NG108-15.
Sukma
Monrudee
M
Division of Medicinal Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama 930-0194, Japan.
Tohda
Michihisa
M
Watanabe
Hiroshi
H
Matsumoto
Kinzo
K
D000243:Adenosine Deaminase / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D017393:RNA Editing; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins; D044405:Receptor, Serotonin, 5-HT2C / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D044405:Receptor, Serotonin, 5-HT2C; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase