title: Differential effect of gamma-radiation-induced heme oxygenase-1 activity in female and male C57BL/6 mice.
abstract: Ionizing radiation produces reactive oxygen species, which exert diverse biological effects on cells and animals. We investigated alterations of heme oxygenase (HO) and non-protein thiols (NPSH), which are known as two major anti-oxidant enzymes, in female and male C57BL/6 mice in the lung, liver, and brain after whole-body gamma-irradiation with 10 Gy (1-7 days) as well as in the lung after whole-thorax gamma-irradiation (WTI) with 12.5 Gy (1-26 weeks). Most significant alteration of HO activity was observed in the liver, which elevated 250% in males. NPSH level in female liver was increased on the 5th-7th days but decreased in males on the 3rd day. In the lung, the elevation of HO activity in both sexes and the pattern of NPSH change were similar to that of the liver. On the other hand, the increase of HO activity on the 16th week and the decrease of NPSH level on the 2nd week were observed only in male lung after WTI. This study shows that the liver is the most sensitive tissue to gamma-irradiation-induced alterations of HO activity in both female and male mice. In addition, there exists significant differential effect of gamma-irradiation on anti-oxidant system in female and male mice.
authors.affiliation: Laboratory of Radiation Immunology, Korea Institute of Radiological & Medical Sciences, KAERI, Seoul.
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Han
authors.forename: Alexander
authors.initials: A
authors.lastname: Platonov
authors.forename: Medea
authors.initials: M
authors.lastname: Akhalaia
authors.forename: Yeon-Sook
authors.initials: YS
authors.lastname: Yun
authors.forename: Jie-Young
authors.initials: JY
authors.lastname: Song
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000378:metabolism / Q000528:radiation effects; D005260:Female; D005720:Gamma Rays; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects; D008099:Liver / Q000201:enzymology* / Q000378:metabolism / Q000528:radiation effects; D008168:Lung / Q000201:enzymology* / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012737:Sex Factors; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013997:Time Factors; D014916:Whole-Body Irradiation
chemical_list: D012333:RNA, Messenger; D013438:Sulfhydryl Compounds