title: Spoligotyping and Mycobacterium tuberculosis.
abstract: We evaluated the clinical usefulness of spoligotyping, a polymerase chain reaction-based method for simultaneous detection and typing of Mycobacterium tuberculosis strains, with acid-fast bacilli-positive slides from clinical specimens or mycobacterial cultures. Overall sensitivity and specificity were 97% and 95% for the detection of M. tuberculosis and 98% and 96% when used with clinical specimens. Laboratory turnaround time of spoligotyping was less than that for culture identification by a median of 20 days. In comparison with IS6110-based restriction fragment length polymorphism typing, spoligotyping overestimated the number of isolates with identical DNA fingerprints by approximately 50%, but showed a 100% negative predictive value. Spoligotyping resulted in the modification of ongoing antimycobacterial treatment in 40 cases and appropriate therapy in the absence of cultures in 11 cases. The rapidity of this method in detection and typing could make it useful in the management of tuberculosis in a clinical setting.
authors.affiliation: Institute of Infectious Diseases, L. Sacco Hospital, University of Milan, Milan, Italy. andrea.gori@unimi.it
authors.forename: Andrea
authors.initials: A
authors.lastname: Gori
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bandera
authors.forename: Giulia
authors.initials: G
authors.lastname: Marchetti
authors.forename: Anna
authors.initials: A
authors.lastname: Degli Esposti
authors.forename: Lidia
authors.initials: L
authors.lastname: Catozzi
authors.forename: Gian Piero
authors.initials: GP
authors.lastname: Nardi
authors.forename: Lidia
authors.initials: L
authors.lastname: Gazzola
authors.forename: Giulio
authors.initials: G
authors.lastname: Ferrario
authors.forename: Jan D A
authors.initials: JD
authors.lastname: van Embden
authors.forename: Dick
authors.initials: D
authors.lastname: van Soolingen
authors.forename: Mauro
authors.initials: M
authors.lastname: Moroni
authors.forename: Fabio
authors.initials: F
authors.lastname: Franzetti
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013997:Time Factors; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial