Hippocampal NMDA receptor subunit expression and watermaze learning in estrogen deficient female mice.
The aromatase knockout (ArKO) mouse is estrogen deficient. Using reverse-transcription and real-time PCR, we showed that transcript levels of the N-methyl-d-aspartate (NMDA) receptor subunit NR2B are significantly higher in the hippocampus of female ArKO mice compared to wild-type (WT) littermates. Expression levels of NR1, NR2A, but not NR2C, also tended to be higher in ArKO mice. In the Morris watermaze test for spatial memory, both genotypes displayed equal significant improvement in the latency in locating the invisible platform over the 5-day training period. These findings show that selective loss of estrogen synthesis is associated with changes in NMDA receptor subunit expression in the hippocampus but little change in spatial learning ability.
Boon
Wah Chin
WC
Prince Henry's Institute of Medical Research, P.O. Box 5152, Clayton, VIC 3168, Australia. wah.chin.boon@phimr.monash.edu.au
Diepstraten
Jeroen
J
van der Burg
Jorien
J
Jones
Margaret E E
ME
Simpson
Evan R
ER
van den Buuse
Maarten
M
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004967:Estrogens / Q000172:deficiency*; D005260:Female; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000502:physiology*; D018782:Maze Learning / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016133:Polymerase Chain Reaction; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*
D017931:DNA Primers; D004967:Estrogens; C409466:NR1 NMDA receptor; C121001:NR2B NMDA receptor; C432823:NR2C NMDA receptor; D016194:Receptors, N-Methyl-D-Aspartate