Salmonella typhimurium grown in iron-rich media, inactivated with ferric chloride and adjuvanted with homologous bacterial DNA is potent and efficacious vaccine in mice.
The present study describes our attempt to construct a novel vaccine formulation that affords full protection against murine typhoid under experimental conditions. Ferric chloride, 100mM, as inactivating agent, bacterial growth under iron-rich conditions and homologous bacterial DNA as adjuvant were used for construction of the experimental Salmonella typhimurium vaccine. The vaccine inoculated twice at 2 weeks interval in Swiss albino mice elicited statistically significant IgG levels when compared with non-adjuvanted and other control groups. All the mice inoculated with the novel vaccine withstood challenge with 50 LD(50) dose of S. typhimurium strain St 585. No significant safety problems were found in mice.
Kumar
Devender
D
Immunology Section, Department of Veterinary Microbiology, College of Veterinary Sciences, Chaudhary Charan Singh Haryana Agricultural University, Hisar 125004, Haryana, India. devender601@rediffmail.com
Singh
Ajit
A
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000917:Antibody Formation; D002712:Chlorides; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000008:administration & dosage*; D005290:Ferric Compounds / Q000494:pharmacology*; D007501:Iron / Q000378:metabolism*; D007928:Lethal Dose 50; D051379:Mice; D012480:Salmonella Infections / Q000276:immunology / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000008:administration & dosage* / Q000276:immunology; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D014611:Vaccination
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D002712:Chlorides; D003470:Culture Media; D004269:DNA, Bacterial; D005290:Ferric Compounds; D022562:Salmonella Vaccines; D007501:Iron; C024555:ferric chloride