LEAFY COTYLEDON 2 activation is sufficient to trigger the accumulation of oil and seed specific mRNAs in Arabidopsis leaves.
LEAFY COTYLEDON 2 (LEC2) is a key regulator of seed maturation in Arabidopsis. To unravel some of its complex pleiotropic functions, analyses were performed with transgenic plants expressing an inducible LEC2:GR protein. The chimeric protein is functional and can complement lec2 mutation. Interestingly, the induction of LEC2 leads to the accumulation of storage oil in leaves. In addition, short-term induction and use of translation inhibitors allowed to demonstrate that LEC2 can directly trigger the accumulation of seed specific mRNAs. Consistent with these results, the expression of three other major seed regulators namely, LEC1, FUS3, and ABI3 were also induced by LEC2 activation.
Santos Mendoza
Monica
M
Seed Biology Laboratory, UMR 204 INRA/INAPG, Institut Jean-Pierre Bourgin, INRA, RD10, 78026 Versailles Cedex, France.
Dubreucq
Bertrand
B
Miquel
Martine
M
Caboche
Michel
M
Lepiniec
Loïc
L
D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D050356:Lipid Metabolism; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics*; D010938:Plant Oils; D012333:RNA, Messenger / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
C091601:ABI3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D022762:CCAAT-Enhancer-Binding Proteins; C502726:FUSCA3 protein, Arabidopsis; C113191:LEC1 protein, Arabidopsis; C504240:LEC2 protein, Arabidopsis; D010938:Plant Oils; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors