title: Structure of equine 2'-5'oligoadenylate synthetase (OAS) gene family and FISH mapping of OAS genes to ECA8p15-->p14 and BTA17q24-->q25.
abstract: Mammalian 2'-5' oligoadenylate (2-5A) synthetases are important mediators of the antiviral activity of interferons. Both human and mouse 2-5A synthetase gene families encode four forms of enzymes: small, medium, large and ubiquitin-like. In this study, the structures of four equine OAS genes were determined using DNA sequences derived from fifteen cDNA and four BAC clones. Composition of the equine OAS gene family is more similar to that of the human OAS family than the mouse Oas family. Two OAS-containing bovine BAC clones were identified in GenBank. Both equine and bovine BAC clones were physically assigned by FISH to horse and cattle chromosomes, ECA8p15-->p14 and BTA17q24--> q25, respectively. The comparative mapping data confirm conservation of synteny between ungulates, humans and rodents.
authors.affiliation: Biology Department, Georgia State University, Atlanta, GA 30302-4010, USA. aperelygin@gsu.edu
authors.forename: A A
authors.initials: AA
authors.lastname: Perelygin
authors.forename: T L
authors.initials: TL
authors.lastname: Lear
authors.forename: A A
authors.initials: AA
authors.lastname: Zharkikh
authors.forename: M A
authors.initials: MA
authors.lastname: Brinton
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D006736:Horses / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D005810:Multigene Family; D012330:RNA, Double-Stranded / Q000235:genetics
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012330:RNA, Double-Stranded; D015088:2',5'-Oligoadenylate Synthetase