title: Using biosensors to detect the release of serotonin from taste buds during taste stimulation.
abstract: CHO cells transfected with high-affinity 5HT receptors were used to detect and identify the release of serotonin from taste buds. Taste cells release 5HT when depolarized or when stimulated with bitter, sweet, or sour tastants. Sour- and depolarization-evoked release of 5HT from taste buds is triggered by Ca2+ influx from the extracellular fluid. In contrast, bitter- and sweet-evoked release of 5HT is triggered by Ca2+ derived from intracellular stores.
authors.affiliation: Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, FL 33136, USA.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Maruyama
authors.forename: K S
authors.initials: KS
authors.lastname: Lu
authors.forename: E
authors.initials: E
authors.lastname: Pereira
authors.forename: I
authors.initials: I
authors.lastname: Plonsky
authors.forename: J E
authors.initials: JE
authors.lastname: Baur
authors.forename: D
authors.initials: D
authors.lastname: Wu
authors.forename: S D
authors.initials: SD
authors.lastname: Roper
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D016466:CHO Cells; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D006224:Cricetinae; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D016257:Fura-2; D007202:Indicators and Reagents; D051379:Mice; D009924:Organ Culture Techniques; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism* / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D013649:Taste / Q000502:physiology*; D013650:Taste Buds / Q000187:drug effects / Q000378:metabolism*
chemical_list: D007202:Indicators and Reagents; D011985:Receptors, Serotonin; C078730:serotonin 5 receptor; D012701:Serotonin; D000255:Adenosine Triphosphate; D016257:Fura-2