Voltammetric study of interaction between polymers (PEI and TMO) and pDNA on a hanging mercury drop electrode.
Electrochemical DNA biosensors can become a powerful tool for the investigation of potent changes on the plasmid DNA structure caused by polymers used as non-viral vectors in gene delivery. Trimethylated chitosan oligomer (TMO) and polyethylenimine (PEI), offering biocompatibility, low immunogenicity and minimal cytotoxicity, are being studied as model non-viral carriers. The information obtained is intended to serve as a basis for developing a new analytical system for the study of the effect of any physically or chemically synthesized polymer on DNA structure.
Gherghi
I Ch
ICh
Analytical Chemistry Laboratory, Department of Chemistry, Aristotle University of Thessaloniki, Greece.
Girousi
S Th
ST
Thanou
M
M
Voulgaropoulos
A N
AN
Tzimou-Tsitouridou
R
R
D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004566:Electrodes; D008628:Mercury / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*
D011094:Polyethyleneimine; D004247:DNA; D048271:Chitosan; D008628:Mercury