Identification of genetically modified potato (Solanum tuberosum) cultivars using event specific polymerase chain reaction.
Several genetically modified (GM) cultivars are registered in Canada although they are not currently in commercial production. The GM cultivars can be distinguished from the non-GM and other GM cultivars by analyzing the DNA nucleotide sequence at the insertion site of the transgene corresponding to a single transformation event in the plant genome. Techniques based on modified polymerase chain reaction (PCR) strategies were used to generate sequence information from the plant genome flanking the insertion site of transgenic DNA for specific GM potato events. The plant genome sequence adjacent to the transgenic insertion was used to design PCR primers, which could be used in combination with a primer annealing to one of the nearby inserted genetic elements to amplify an event specific DNA fragment. The event specific PCR fragments generated were sequenced to confirm the specificity of the method.
Côté
Marie-José
MJ
Center for Plant Quarantine Pests, Ottawa Laboratory (Fallowfield), Canadian Food Inspection Agency, 3851 Fallowfield Road, Ottawa, Ontario K2H 8P9, Canada. cotemj@inspection.gc.ca
Meldrum
Allison J
AJ
Raymond
Philippe
P
Dollard
Cheryl
C
D018744:DNA, Plant / Q000032:analysis*; D030821:Plants, Genetically Modified / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000235:genetics*
D018744:DNA, Plant