title: Relative retention order of all isomers of cis/trans conjugated linoleic acid FAME from the 6,8- to 13,15-positions using silver ion HPLC with two elution systems.
abstract: CLA, defined as one or more octadecadienoic acids (18:2) with conjugated double bonds, has been reported to be active in a number of biological systems. GC and silver ion HPLC (Ag(+)-HPLC) have been the primary techniques for identifying specific CLA isomers in both foods and biological extracts. Recently, GC relative retention times were reported for all c,c, c/t (c,t and tc), and t,t CLA FAME from the 6,8- to the 13,15-positions in octadecadienoic acid (18:2). Presented here is the relative retention order of the same CLA FAME using Ag(+)-HPLC with two different elution systems. The first elution system, consisting of 0.1% acetonitrile/0.5% diethyl ether (DE)/hexane, has been used previously to monitor CLA composition in foods. Also presented here is the retention order of CLA FAME using 2% acetic acid/hexane elution solvent, which has advantages of more stable retention volumes and a complementary elution order of CLA FAME isomers. The data are reported using retention volumes (RV) adjusted for toluene, an estimator for dead volume, and relative to c9,t11-18:2. Measurement of relative RV in the analysis of 88 samples of cow plasma, milk, and rumen fluids using Ag(+)-HPLC is also presented here. The % CV ranged from 1.04 to 1.62 for t,t isomers and from 0 to 0.48 for c/t isomers.
authors.affiliation: US Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, Maryland 20740, USA.
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Delmonte
authors.forename: Ai
authors.initials: A
authors.lastname: Kataok
authors.forename: B A
authors.initials: BA
authors.lastname: Corl
authors.forename: D E
authors.initials: DE
authors.lastname: Bauman
authors.forename: Martin P
authors.initials: MP
authors.lastname: Yurawecz
mesh_terms: D000097:Acetonitriles; D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004986:Ether; D007536:Isomerism; D044243:Linoleic Acids, Conjugated / Q000097:blood / Q000302:isolation & purification*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012417:Rumen / Q000737:chemistry; D012834:Silver
chemical_list: D000097:Acetonitriles; D044243:Linoleic Acids, Conjugated; D004986:Ether; D012834:Silver; C032159:acetonitrile