title: Bone marrow lacks a transplantable progenitor for smooth muscle type alpha-actin-expressing cells.
abstract: While some studies have suggested that hematopoietic stem cells might give rise to other tissue types, others indicate that transdifferentiation would have to be an extremely rare event. We have now exploited smooth muscle type alpha-actin (alphaSMA) promoter-driven green fluorescent protein (GFP) transgenic mice (alphaSMA-GFP mice) for bone marrow transplantation to evaluate their potential to generate donor-type tissues in irradiation chimeras. There was a highly restricted pattern of GFP expression in the transgenic mice, marking bone marrow stromal cells and mesangial cells in the kidney. However, these characteristics were not transferable to wild-type animals given transgenic marrow cells even though hematopoietic cells were largely replaced. Our findings support earlier studies suggesting that the bone marrow microenvironment is difficult to transplant and indicate that hematopoietic stem cells are unlikely to give rise to alphaSMA-expressing progeny.
authors.affiliation: Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73190-3048, USA.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Yokota
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Nagai
authors.forename: Jian-xing
authors.initials: JX
authors.lastname: Ma
authors.forename: Jen-Yue
authors.initials: JY
authors.lastname: Tsai
authors.forename: Paul W
authors.initials: PW
authors.lastname: Kincade
authors.forename: Sanai
authors.initials: S
authors.lastname: Sato
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D050527:Mesangial Cells / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D017154:Stromal Cells; D018183:Transplantation Chimera
chemical_list: D000199:Actins; D049452:Green Fluorescent Proteins