title: Molecular cloning, expression analysis and assignment of the porcine tumor necrosis factor superfamily member 10 gene (TNFSF10) to SSC13q34-->q36 by fluorescence in situ hybridization and radiation hybrid mapping.
abstract: We have cloned the complete coding region of the porcine TNFSF10 gene. The porcine TNFSF10 cDNA has an ORF of 870 nucleotides and shares 85% identity with human TNFSF10, and 75% and 72% identity with rat and mouse Tnfsf10 coding sequences, respectively. The deduced porcine TNFSF10 protein consists of 289 amino acids with the calculated molecular mass of 33.5 kDa and a predicted pI of 8.15. The amino acid sequence similarities correspond to 86, 72 and 70% when compared with human, rat and mouse sequences, respectively. Northern blot analysis detected TNFSF10-specific transcripts (approximately 1.7 kb) in various organs of a 10-week-old pig, suggesting ubiquitous expression. Real-time RT-PCR studies of various organs from fetal (days 73 and 98) and postnatal stages (two weeks, eight months) demonstrated developmental and tissue-specific regulation of TNFSF10 mRNA abundance. The chromosomal location of the porcine TNFSF10 gene was determined by FISH of a specific BAC clone to metaphase chromosomes. This TNFSF10 BAC clone has been assigned to SSC13q34-->q36. Additionally, the localization of the TNFSF10 gene was verified by RH mapping on the porcine IMpRH panel.
authors.affiliation: Institute for Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig Maximilian University, Munich, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Kemter
authors.forename: U
authors.initials: U
authors.lastname: Philipp
authors.forename: R
authors.initials: R
authors.lastname: Klose
authors.forename: H
authors.initials: H
authors.lastname: Kuiper
authors.forename: M
authors.initials: M
authors.lastname: Boelhauve
authors.forename: O
authors.initials: O
authors.lastname: Distl
authors.forename: E
authors.initials: E
authors.lastname: Wolf
authors.forename: T
authors.initials: T
authors.lastname: Leeb
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D021101:Radiation Hybrid Mapping / Q000379:methods; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D017931:DNA Primers; D014409:Tumor Necrosis Factor-alpha