Resistin SNP-420 determines its monocyte mRNA and serum levels inducing type 2 diabetes.
Resistin, secreted from adipocytes, causes insulin resistance in rodents. Its roles and main source in humans remain unknown. The G/G genotype of resistin single nucleotide polymorphism, SNP-420, induces type 2 diabetes mellitus (T2DM) by increasing promoter activity. We elucidated factors correlated with serum resistin and effects of SNP-420 on monocyte resistin mRNA. In 198 T2DM and 157 controls, fasting serum resistin was higher in T2DM. Multiple regression analysis revealed that SNP-420 genotype was the strongest determinant of serum resistin. In T2DM, 1-year duration of T2DM and 1% HbA1c was also correlated with 0.19 and 0.54 ng/ml serum resistin, respectively. Logistic regression analysis revealed that serum resistin was an independent factor for T2DM. In 23 healthy volunteers, monocyte resistin mRNA was positively correlated with its simultaneous serum levels and was higher in G/G genotype. Thus, SNP-420 determines monocyte mRNA and serum levels of resistin, which could induce T2DM.
Osawa
Haruhiko
H
Department of Laboratory Medicine, Ehime University School of Medicine, Shitsukawa, Toon, Ehime 791-0295, Japan. harosawa@m.ehime-u.ac.jp
Onuma
Hiroshi
H
Ochi
Masaaki
M
Murakami
Akiko
A
Yamauchi
Junko
J
Takasuka
Tomomi
T
Tanabe
Fumiko
F
Shimizu
Ikki
I
Kato
Kenichi
K
Nishida
Wataru
W
Yamada
Kazuya
K
Tabara
Yasuharu
Y
Yasukawa
Masaki
M
Fujii
Yasuhisa
Y
Ohashi
Jun
J
Miki
Tetsuro
T
Makino
Hideichi
H
D017667:Adipocytes / Q000378:metabolism; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004247:DNA / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000235:genetics*; D005260:Female; D005838:Genotype; D006729:Hormones, Ectopic / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D012044:Regression Analysis; D052243:Resistin; D017422:Sequence Analysis, DNA
D006729:Hormones, Ectopic; C499624:RETN protein, human; D012333:RNA, Messenger; D052243:Resistin; D004247:DNA