Subcellular characterization of the primordial germ cell antigen PG2 in adult oocytes.
Primordial germ cells represent the founder population for establishing the germ line providing the continuity of life between generations. PG2, a germ cell-specific antigen, is one of the few continuously detectable epitopes in mammalian primordial germ cells and it is dynamically expressed during early post-fertilization development and during postnatal germ cell maturation. Immunoelectron microscopy shows a localization of PG2 in the peri-mitochondrial cytoplasm but its further subcellular or biochemical nature remains elusive. For further characterization of the PG2 epitope we used regular and semi-thin cryosection of ovulated and isolated follicular rabbit oocytes and localized all mitochondria with the help of the constitutive mitochondrial antigen MTC02 in double immunofluorescence stainings. Semi-thin cryosections of ovulated oocytes revealed a general close co-localization of both antibody reactions at the level of single mitochondria. In centrifuged follicular oocytes both antigens co-sedimented almost completely indicating a topographical association of the epitopes on the basis of a strong interaction of PG2 with mitochondria. To begin to characterize the germ cell epitope biochemically we treated oocyte cryosections either with acetone to reduce lipids or with N-glycosidase F to remove N-linked glycosylations before the immuoreaction. Neither treatment affected the antibody characteristics, which suggests that the PG2 epitope is most probably a protein. Because of the close interaction of PG2 with the mitochondria we speculate that PG2 is involved in the change of the mitochondrial morphology typically observed during differentiation of germ cells.
Püschel
Bernd
B
Zentrum Anatomie, Abteilung Anatomie und Embryologie, Georg-August-Universität Göttingen, Kreuzbergring 36, 37075, Göttingen, Germany.
Demus
Uta
U
Viebahn
Christoph
C
D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000276:immunology / Q000378:metabolism*; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D008928:Mitochondria / Q000276:immunology / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism* / Q000648:ultrastructure; D011817:Rabbits
D000906:Antibodies; D000941:Antigens; D000939:Epitopes