Genetic characterization of Cryptosporidium isolates from ringed seals (Phoca hispida) in Northern Quebec, Canada.
This study reports the molecular characterization of Cryptosporidium spp. isolates identified from intestinal contents of ringed seals (Phoca hispida) from Nunavik (Quebec, Canada). Cryptosporidium spp. fragments of 18S rRNA, HSP-70, and actin loci were amplified by PCR from seal intestinal contents. PCR-positive specimens were sequenced and compared with other Cryptosporidium species and genotypes reported previously. Sequence analysis showed the presence of C. muris and 2 novel genotypes in ringed seals.
Santín
Mónica
M
Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA.
Dixon
Brent R
BR
Fayert
Ronald
R
D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D016054:DNA, Protozoan / Q000737:chemistry; D005260:Female; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007422:Intestines / Q000469:parasitology; D008297:Male; D008969:Molecular Sequence Data; D046049:Phoca / Q000469:parasitology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011792:Quebec; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D016415:Sequence Alignment / Q000662:veterinary; D015047:Zoonoses / Q000469:parasitology / Q000635:transmission
D000199:Actins; D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D012337:RNA, Ribosomal, 18S