Mouse anterior pituitary gland: analysis by ion trap mass spectrometry.
We investigated the proteome of the anterior pituitary gland (AP) in a species in which the genome has been sequenced. Subcellular fractions of APs from 2-month-old male mice were prepared for protein denaturation, treatment with trypsin and analyses utilizing micro liquid chromatography tandem mass spectrometry and the database search software SEQUEST. In the nuclear, non-nuclear 100,000 g and cytosolic fractions, we identified 49, 36 and 68 different proteins, respectively. A total of 115 distinct proteins were detected. We identified growth hormone, prolactin, pro-opiomelanocortin, the alpha-subunit for the glycoprotein hormones, and luteinizing hormone-beta. Groups of other identified proteins included hormone-processing, secretion granule-associated, non-hormonal endoplasmic reticulum-associated, calcium-binding, protein kinase C-associated, histones, non-histone chromosomal, other RNA-binding, heterogeneous nuclear ribonucleoproteins, splicing factors, helicases, lamins, ribosomal, microtubule-associated, microfilament-associated, adenosine triphosphate- and guanosine triphosphate-associated, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation, enzymes in glycolysis and the tricarboxylic and urea cycles and the pentose phosphate path, heat-shock, glutathione-associated, peroxidases, ubiquitin-associated, catabolic, protease inhibitors, other, and blood proteins. The 115 proteins reported in this study and the 145 proteins reported in a previous study on the AP of the adult male Golden Syrian hamster are compared and form a foundation for defining the proteome in normal adult male AP.
Blake
Charles A
CA
Department of Cell and Developmental Biology and Anatomy, University of South Carolina School of Medicine, Columbia, 29208, USA. Blake@med.sc.edu
Kakhniashvili
David G
DG
Goodman
Steven R
SR
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D002853:Chromatography, Liquid; D006224:Cricetinae; D003600:Cytosol / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008647:Mesocricetus; D051379:Mice; D010903:Pituitary Gland, Anterior / Q000737:chemistry* / Q000648:ultrastructure; D010907:Pituitary Hormones / Q000032:analysis; D011489:Protein Denaturation; D020543:Proteome / Q000032:analysis*; D012984:Software; D014357:Trypsin / Q000378:metabolism
D010907:Pituitary Hormones; D020543:Proteome; D014357:Trypsin