[Isolation and characterization of new species hydrogen producing bacterium Ethanologenbacterium sp. strain X-1 and its capability of hydrogen production].
To obtain hydrogen-producing bacterium of high efficiency, a strain X-1 of hydrogen-producing bacteria was isolated from the continuous stirred-tank reactor (CSTR) by anaerobic Hungate technique. The Comparative sequence analysis of 16S rDNA showed that homology of strain X-1 with Clostridium cellulose and Acetanaerobacterium elongatum is less than 94%. All sequence alignment of 16S-23S rDNA intergenic spacer regions (ISR) indicated displayed that consensus region is tRNA(Ala), and tRNA(Ile), variable region is not homologous. Morphological, physic-biochemical character, and comparative sequence analysis of 16S rDNA and 16S-23S rDNA ISR indicated that strain X-1 belong to new genus named Ethanologenbacterium gen. nov.. Strain X-1 is facultative anaerobe bacillus; its main fermentative products are acetic acid, ethanol, H2 and CO2. The metabolic character of strain X-1 is typical ethanol type fermentation. Its capability of hydrogen production was measured in the batch culture experiment. X-1's maximum specific hydrogen producing rate is 28.3 mmol H2/( g dry cell x h) at pH 4.0 and 36 degrees C. Result of identify and analysis of hydrogen production ability demonstrated strain X-1 belong to new genus of high hydrogen-producing bacteria.
Xing
De-Feng
DF
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. ixdf@yahoo.com.cn
Ren
Nan-Qi
NQ
Li
Qiu-Bo
QB
D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature
D012336:RNA, Ribosomal, 16S; D000431:Ethanol; D006859:Hydrogen