title: Imaging proteins with atomic force microscopy: an overview.
abstract: Atomic force microscopy (AFM) has become a common tool for biophysical studies of proteins; mainly due its property to perform characterizations near physiological conditions. The tertiary and quaternary structures, forces driving folding-unfolding processes, and secondary structure elements can be studied in their native environments allowing high resolution level associated with small distortions. This review outlines the operational principles and applications of AFM for protein biophysics.
authors.affiliation: Departamento de Genética e Morfologia, Universidade de Brasilia, 70910-900, Brasília, DF, Brazil. paulinoluciano@unb.br
authors.forename: Luciano Paulino
authors.initials: LP
authors.lastname: Silva
mesh_terms: D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D008024:Ligands; D018625:Microscopy, Atomic Force / Q000379:methods*; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D008024:Ligands; D011506:Proteins; D004247:DNA