Mediator lipidomics.
Lipidomics the systematic decoding of lipid-based information in biosystems is comprised of identification and profiling of lipids and lipid-derived mediators. As practiced today, lipidomics can be subdivided into architecture/membrane-lipidomics and mediator-lipidomics. The mapping of structural components and their relation to cell activation as well as generation of potent lipid mediators and networks involves a mass spectrometry-computational approach to appreciate inter-relationships and complex mediator networks important for cell homeostasis. Cell membranes are composed of a bilayer that contains phospholipids, fatty acids, integral membrane proteins, and membrane associated proteins, sphingolipids, etc. Membrane composition of many cell types is established. However, their organization and how they affect cell function remains an area of interest and a quest for lipidomics. Membranes serve barrier functions separating the inside from outside or compartments within cells, regulating passage of nutrients, gasses, and specific ions as well as generate signals to the intracellular milieu by the membrane's ability to interact with key proteins. The nature of these interactions and decoding the structure-function information within their organization is the promise of lipidomics (A-C). Metabolism of fatty acids is also an important energy source; hence, catabolism breakdown of fatty acids, areas of metabolomics that link to the signaling pathways, and roles of lipid mediators discussed herein.
Serhan
Charles N
CN
Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA. cnserhan@zeus.bwh.harvard.edu
D000465:Algorithms; D000818:Animals; D030801:Animals, Genetically Modified; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D007249:Inflammation; D007477:Ions; D008051:Lipid Bilayers / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D013058:Mass Spectrometry; D008954:Models, Biological; D008956:Models, Chemical; D008958:Models, Molecular; D010743:Phospholipids / Q000737:chemistry; D011453:Prostaglandins / Q000737:chemistry; D011487:Protein Conformation; D015398:Signal Transduction; D012984:Software; D019076:Transgenes
D005227:Fatty Acids; D007477:Ions; D008051:Lipid Bilayers; D008055:Lipids; D010743:Phospholipids; D011453:Prostaglandins