Non-biofouling materials prepared by atom transfer radical polymerization grafting of 2-methacryloloxyethyl phosphorylcholine: separate effects of graft density and chain length on protein repulsion.
Biomimetic poly(2-methacryloyloxyethyl phosphorylcholine) (poly(MPC)) brushes with graft density 0.06-0.39 chains/nm2 and chain length 5-200 monomer units were prepared from silicon wafer surfaces by combining self-assembly of initiator and surface-initiated atom transfer radical polymerization (ATRP). Water contact angle, X-ray photoelectron spectroscopy, and atomic force microscopy were used to characterize the modified surfaces. These surfaces with well-controlled poly(MPC) brushes were tested for protein repelling performance. Fibrinogen adsorption from tris-buffered saline at pH 7.4 decreased significantly with increasing graft density and/or chain length of poly(MPC) and reached a level of < 10 ng/cm2 at graft density > or = 0.29 chains/nm2 and chain length > or = 100 units, compared to ca. 570 ng/cm2 for the unmodified samples. While the fibrinogen adsorption was determined by both graft density and chain length, it showed a stronger dependence on graft density than on chain length.
Feng
Wei
W
Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ont., Canada L8S 4L7.
Brash
John L
JL
Zhu
Shiping
S
D002021:Buffers; D005340:Fibrinogen / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D011109:Polymethacrylic Acids; D012825:Silicon / Q000737:chemistry; D014867:Water
D002021:Buffers; D005609:Free Radicals; D008689:Methacrylates; D011109:Polymethacrylic Acids; C115766:poly(2-methacryloyloxyethyl-phosphorylcholine); D014867:Water; D010767:Phosphorylcholine; D005340:Fibrinogen; D012825:Silicon