title: Spectral study of interactions of 4,8,4'-trimethylpsoralen and 4,4'-dimethylangelicin dyes with DNA.
abstract: Absorption and fluorescence spectra for six new synthetic dyes of 4,8,4'-trimethylpsoralen and 4,4'-dimethylangelicin derivatives containing various terminal substituents at 5'-position have been investigated in different environments using a wide range of the DNA/ligand concentrations. Various spectral and binding characteristics of the DNA-ligand systems have been determined. General principles characterizing mechanisms responsible for changes in the fluorescent properties of nucleotide-specific dyes have been proposed; they take into consideration chemical structure of the dyes, properties of the environment, and degree of sorption on substrate.
authors.affiliation: Mendeleyev Russian Research Institute for Metrology, St. Petersburg, 190005, Russia. vs1969r@mail.ru
authors.forename: V S
authors.initials: VS
authors.lastname: Sibirtsev
authors.forename: A Yu
authors.initials: AY
authors.lastname: Tolmachev
authors.forename: M V
authors.initials: MV
authors.lastname: Kovaleva
authors.forename: A V
authors.initials: AV
authors.lastname: Garabadzhiu
authors.forename: V F
authors.initials: VF
authors.lastname: Traven
mesh_terms: D004396:Coloring Agents / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D011564:Furocoumarins / Q000138:chemical synthesis / Q000737:chemistry*; D008024:Ligands; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods; D014307:Trioxsalen / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D011564:Furocoumarins; D008024:Ligands; C038161:4,4'-dimethylangelicin; D004247:DNA; D014307:Trioxsalen