Translocation of double-strand DNA through a silicon oxide nanopore.
We report double-strand DNA translocation experiments using silicon oxide nanopores with a diameter of about 10 nm . By monitoring the conductance of a voltage-biased pore, we detect molecules with a length ranging from 6557 to 48 500 base pairs. We find that the molecules can pass the pore both in a straight linear fashion and in a folded state. Experiments on circular DNA further support this picture. We sort the molecular events according to their folding state and estimate the folding position. As a proof-of-principle experiment, we show that a nanopore can be used to distinguish the lengths of DNA fragments present in a mixture. These experiments pave the way for quantitative analytical techniques with solid-state nanopores.
Storm
A J
AJ
Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands.
Chen
J H
JH
Zandbergen
H W
HW
Dekker
C
C
D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry; D001692:Biological Transport; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D008422:Materials Testing; D008567:Membranes, Artificial; D009038:Motion; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D012822:Silicon Dioxide; D004247:DNA