title: MonoADP-ribosylation of the NAD+-dependent alcohol dehydrogenase from Entamoeba histolytica.
abstract: The human parasite Entamoeba histolytica is an amitochondrial protozoan whose metabolism depends on glucose fermentation. Among the metabolic enzymes absolutely required for amoeba growth is the NAD+-dependent alcohol dehydrogenase (EhADH2). The polymeric form of EhADH2 was sedimented at 160,000 g, and in this fraction we observed [32P]-labeling of a 96-kDa protein under mono-ADP-ribosylation conditions with [32P]NAD+. The [32P]-labeled protein had the same molecular weight as the EhADH2 monomer. Because of the importance of monoADP-ribosylation in the regulation of many physiological processes, the aim of this study was to determine whether EhADH2 is ADP-ribosylated, and what would be the consequence of this modification on its alcohol and aldehyde dehydrogenase enzymatic activities. This study describes the ADP-ribosylation of EhADH2. This modification did not have an effect on the enzymatic activities, but it may regulate other functions of EhADH2.
authors.affiliation: Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato, P. O. Box 187, Guanajuato, Gto, México, CP36000.
authors.forename: Susana M L
authors.initials: SM
authors.lastname: Fuentes
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Martínez-Cadena
authors.forename: Mónica E
authors.initials: ME
authors.lastname: Silva
authors.forename: Araceli
authors.initials: A
authors.lastname: López
authors.forename: Carmen
authors.initials: C
authors.lastname: Sánchez
authors.forename: Angel H
authors.initials: AH
authors.lastname: Alvarez
authors.forename: Eva E
authors.initials: EE
authors.lastname: Avila
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002498:Centrifugation; D004748:Entamoeba histolytica / Q000378:metabolism*; D008970:Molecular Weight; D009243:NAD / Q000031:analogs & derivatives* / Q000378:metabolism*
chemical_list: D009243:NAD; D000426:Alcohol Dehydrogenase