Radial compression elasticity of single DNA molecules studied by vibrating scanning polarization force microscopy.
The radial compression properties of single DNA molecules have been studied using vibrating scanning polarization force microscopy. By imaging DNA molecules at different vibration amplitude set-point values, we obtain the correlations between radially applied force and DNA compression, from which the radial compressive elasticity can be deduced. The estimated elastic modulus is approximately 20-70 MPa under small external forces (<0.4 nN) and increases to approximately 100-200 MPa for large loads.
Zhou
Xing-Fei
XF
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
Sun
Jie-Lin
JL
An
Hong-Jie
HJ
Guo
Yun-Chang
YC
Fang
Hai-Ping
HP
Su
Chanmin
C
Xiao
Xu-Dong
XD
Huang
Wen-Hao
WH
Li
Min-Qian
MQ
Shen
Wen-Qing
WQ
Hu
Jun
J
D019245:Compressive Strength; D003198:Computer Simulation; D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D004548:Elasticity; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008846:Micromanipulation / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008859:Microscopy, Polarization / Q000379:methods*; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D013314:Stress, Mechanical; D014732:Vibration
D004247:DNA