Primers for clinical detection of Paracoccidioides brasiliensis.
From a 0.72-kb fragment universally generated in Paracoccidioides brasiliensis strains, primers were designed and tested on genomic DNA of this and other pathogenic fungi. They were specific and highly sensitive for P. brasiliensis DNA. Positive results were obtained when these were tested in clinical samples.
San-Blas
Gioconda
G
Instituto Venezolano de Investigaciones Científicas, Centro de Microbiología y Biología Celular, Apartado 21827, Caracas 1020A, Venezuela. sanblasg@ivic.ve
Niño-Vega
Gustavo
G
Barreto
Laura
L
Hebeler-Barbosa
Flavia
F
Bagagli
Eduardo
E
Olivero de Briceño
Rosa
R
Mendes
Rinaldo Poncio
RP
D000328:Adult; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010228:Paracoccidioides / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004271:DNA, Fungal