Equine FISH mapping of 36 genes known to locate on human chromosome ends.
The INRA and the CHORI-241 horse BAC libraries were screened by hybridization with DNA probes and/or directly by PCR with primers designed in consensus sequences of genes localized at the end of each human chromosome. BAC clones were retrieved and 36 could be FISH mapped after the expected gene was confirmed in each BAC by sequencing. Our results show that 16 BACs can be considered to be at telomeric or centromeric positions in the horse and 15 were found at the boundary of actually defined conserved segments even-though often located within conserved syntenic fragments between horse and human. There is no straightforward relation between the end position of a marker in human and its end position in the horse. A gene was first anchored to ECA27 by FISH mapping. The localization of these markers expands the cytogenetic map of the horse and will serve as anchors for the integrated and future physical maps. It should also help to better understand the different chromosomal rearrangements that occurred during evolution of genomes derived from a common ancestral karyotype.
Perrocheau
M
M
Département de Génétique Animale, Institut National de la Recherche Agronomique, Centre de Recherches de Jouy, Jouy-en-Josas, France.
Boutreux
V
V
Chadi-Taourit
S
S
Di Meo
G P
GP
Perucatti
A
A
Incarnato
D
D
Cribiu
E P
EP
Guérin
G
G
Iannuzzi
L
L
D001483:Base Sequence; D018929:Cell Culture Techniques; D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial; D002877:Chromosomes, Human; D020732:Cytogenetic Analysis / Q000379:methods; D017931:DNA Primers; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D005819:Genetic Markers