title: Effect of polymer matrix and glycerol on rapid single-strand conformation polymorphism analysis by capillary and microchip electrophoresis for detection of mutations in K-ras gene.
abstract: We present the rapid single-strand conformation polymorphism (SSCP) analysis by capillary and microchip electrophoresis to detect the mutations in K-ras gene. Parameters that might affect the analysis of mutation in K-ras gene, such as the polymer and the additive in the sieving matrix, have been studied systematically. Under the optimal conditions, the analysis of seven mutants of K-ras gene could be finished within 10 min by capillary electrophoresis (CE). Furthermore, with the wild-type gene as the inner standard, the analysis accuracy of mutations could be improved. In addition, by studying the properties of polymer solutions, the matrix suitable for microchip electrophoresis was found, and the detection of mutations in K-ras gene could be further shortened to 1 min.
authors.affiliation: Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Sciences, The University of Tokushima, Tokushima, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Endo
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Rumi
authors.initials: R
authors.lastname: Katashima
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Itakura
authors.forename: Erin A S
authors.initials: EA
authors.lastname: Doherty
authors.forename: Annelise E
authors.initials: AE
authors.lastname: Barron
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D019075:Electrophoresis, Capillary / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000379:methods*; D011905:Genes, ras; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D017931:DNA Primers