title: Nanoparticle-assisted micropatterning of active proteins on solid substrate.
abstract: Micropatterning of proteins on silica substrate was achieved using a new method. Proteins were first immobilized onto silica nanoparticles which were then dispensed into arrayed microwells on silicon. Atomic force microscopy (AFM), fluorescence microscopy and Fourier transform infrared (FTIR) spectroscopy were used to characterize the samples. The results showed that, compared to a planar surface, curved surfaces of nanoparticles provide more space for attaching proteins and thus increases the intensity of fluorescence signal. Furthermore, after attaching to silica nanoparticles, bovine serum albumin (BSA) maintains its major structure and the cytokine IFN-gamma maintains its ability to bind to its antibody. Use of this method can be extended to micropatterning of other biomolecules, such as DNA and enzymes.
authors.affiliation: Division of Bioengineering, Faculty of Engineering, National University of Singapore.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ho Soon
authors.initials: HS
authors.lastname: Seng
authors.forename: Low Lee
authors.initials: LL
authors.lastname: Ngo
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D020099:Coated Materials, Biocompatible; D012710:Serum Albumin, Bovine