Insertional inactivation of determinants for Mg2+ and Co2+ transport as a tool for screening recombinant Lactococcus species clones.
Insertional inactivation of the plasmid-encoded determinants for Mg(2+) and Co(2+) transport, orf18/corA, provides a tool for screening recombinant clones in Lactococcus, based on the observation that overexpression of orf18/corA results in cell growth inhibition on certain concentrations of CoCl(2). The lacticin 3147 immunity gene, ltnI, was used to insertionally inactivate orf18/corA. The resulting clones were capable of growth on concentrations of CoCl(2) that were inhibitory to the parent strain. Since only 3 of 17 lactococcal starters naturally harbor corA, the system has potential as a screen for selecting recombinant lactococcal clones.
Mills
Susan
S
Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, County Cork, Ireland.
Coffey
Aidan
A
Hill
Colin
C
Fitzgerald
Gerald F
GF
McAuliffe
Olivia
O
Ross
R Paul
RP
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001693:Biological Transport, Active; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003035:Cobalt / Q000378:metabolism* / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D017045:Lactococcus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism* / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D027682:Cation Transport Proteins; C072716:CorA protein, Salmonella; D011994:Recombinant Proteins; D003035:Cobalt; D008274:Magnesium