Simple and rapid method for the differentiation of Lens culinaris Medik. from false lentil species.
The practice of using both common vetch (Vicia sativa L.) and single-flower vetch (Vicia articulata Hornem.) seeds as food or feed is encouraged by the very high resemblance of their seeds with those of small-seeded lentil cultivars. Among the Vicieae, antinutritional and toxic factors are particularly important, because many species, containing high levels of these compounds, are not safe. A simple and fast capillary electrophoresis (CE) method was proposed for the differentiation of lentil cultivars from false lentil species (i.e., single-flour vetch and common vetch). Proteins were extracted from defatted milled seeds with an alcoholic/saline solution. Extracts were separated in an uncoated fused-silica capillary with iminodiacetic acid (IDA) isolectric buffer containing 0.05% hydroxypropylmethylcellulose (HPMC) and 20% acetonitrile. The presented method is useful also for the detection of contamination of whole or split seeds of lentil by vetch species. With respect to alternative techniques, such as DNA-based markers or thin-layer chromatography (TLC), CE has the advantages of being less expensive, faster, and fully automated.
Piergiovanni
Angela R
AR
Istituto di Genetica Vegetale-CNR, via Amendola 165/a, 70126 Bari, Italy. angelarosa.piergiovanni@igv.cnr.it
Taranto
Giovanni
G
D019075:Electrophoresis, Capillary; D005506:Food Contamination / Q000032:analysis*; D027806:Lens Plant / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*; D031306:Vicia / Q000737:chemistry
D010940:Plant Proteins