Reversion in vivo after inoculation of a molecular proviral DNA clone of simian immunodeficiency virus with a cytotoxic-T-lymphocyte escape mutation.
Vaccine-based control of the replication of a simian immunodeficiency virus (SIV), SIVmac239, in macaques has recently been shown. In the process of the control, a mutant virus escaping from epitope-specific cytotoxic-T-lymphocyte (CTL) responses was rapidly selected and contained. In this study, we show that the wild-type virus appeared and became predominant in the absence of the epitope-specific CTL after inoculation of naive macaques with a molecular clone DNA of the CTL escape mutant SIV. This is the first report describing reversion in vivo from an inoculated, molecular proviral DNA clone of immunodeficiency virus with a CTL escape mutation.
Kobayashi
Masahiro
M
Department of Microbiology, Graduate School of Medicine, The University of Tokyo, Japan.
Igarashi
Hiroko
H
Takeda
Akiko
A
Kato
Moriaki
M
Matano
Tetsuro
T
D016915:AIDS Vaccines / Q000008:administration & dosage*; D000595:Amino Acid Sequence; D000818:Animals; D004279:DNA, Viral / Q000008:administration & dosage*; D018984:Epitopes, T-Lymphocyte; D015683:Gene Products, gag / Q000235:genetics; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D009154:Mutation; D011533:Proviruses; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000502:physiology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014611:Vaccination; D014779:Virus Replication
D016915:AIDS Vaccines; D004279:DNA, Viral; D018984:Epitopes, T-Lymphocyte; D015683:Gene Products, gag