Unusual accumulation of demethylspheroidene in anaerobic-phototrophic growth of crtA-deleted mutants of Rhodovulum sulfidophilum.
Rhodovulum sulfidophilum produces carotenoids in the spheroidene pathway. Spheroidene monooxygenase, CrtA, catalyzes the conversion of spheroidene to spheroidenone. crtA-deleted mutants of R. sulfidophilum did not produce spheroidenone and demethylspheroidenone. In these mutants, the ratio of demethylspheroidene to spheroidene increased with exposure to light. One mutant exhibiting a spheroidene-predominant phenotype did not grow under anaerobic-light conditions and was devoid of bacteriochlorophyll a, even under semiaerobic-light conditions There was no difference in the growth of the mutants under aerobic-dark conditions. These data suggest that demethylspheroidene is important for photosynthesis in R. sulfidophilum.
Maeda
Isamu
I
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, 565-0871, Japan.
Yamashiro
Hidenori
H
Yoshioka
Daiki
D
Onodera
Masanori
M
Ueda
Shunsaku
S
Miyasaka
Hitoshi
H
Umeda
Fusako
F
Kawase
Masaya
M
Takaichi
Shinichi
S
Yagi
Kiyohito
K
D000693:Anaerobiosis; D002338:Carotenoids / Q000378:metabolism*; D017353:Gene Deletion; D008027:Light; D006899:Mixed Function Oxygenases / Q000172:deficiency / Q000235:genetics; D010788:Photosynthesis; D041903:Rhodovulum / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
C005637:spheroidene; C104880:spheroidenone; D002338:Carotenoids; D006899:Mixed Function Oxygenases