Molecular character of the recombinant antitumor lectin from the edible mushroom Agrocybe aegerita.
The lectin from Agrocybe aegerita (AAL) has been found to possess potent tumor-suppressing function and tumor cell apoptosis-inducing activity. In this paper, we report the full sequence, the active expression of the gene encoding AAL at a high level and bioassay of the binding property with lactose, apoptosis-inducing activity and DNase activity of recombinant AAL (rAAL). The results reveal that AAL is a member of the galectin family and the dimeric form is the active unit for the functional performance. The rAAL showed comparable tumor cell apoptosis-inducing activity with the wild AAL but no DNase activity at all. The molecular characters revealed by this study are significant for the in-depth investigation of the functional mechanism of this interesting protein.
Yang
Na
N
Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing.
Tong
Xin
X
Xiang
Ye
Y
Zhang
Ying
Y
Liang
Yi
Y
Sun
Hui
H
Wang
Da-Cheng
DC
D000363:Agaricales / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D003001:Cloning, Molecular; D018076:DNA, Complementary; D003851:Deoxyribonucleases / Q000378:metabolism; D037161:Galectins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006367:HeLa Cells; D006386:Hemagglutination Tests; D006801:Humans; D007785:Lactose / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000970:Antineoplastic Agents; D018076:DNA, Complementary; D037161:Galectins; D011994:Recombinant Proteins; D003851:Deoxyribonucleases; D007785:Lactose