Immunohistochemical staining of epoxy resin sections of peripheral nerve.
The authors describe a simple and cost-effective method of immunostaining semithin epoxy resin sections of peripheral nerve for light microscopy with antibodies to myelin protein zero, peripheral myelin protein 22, myelin basic protein, and neurofilament protein 200 using a combined technique of surface etching with sodium ethoxide and heat-induced antigen retrieval.
Cai
Zhao
Z
Centre for Neurological Disease, Hanson Institute, Adelaide, Australia.
Manavis
Jim
J
Cash
Kathy
K
Thompson
Philip D
PD
Blumbergs
Peter C
PC
D000906:Antibodies; D004853:Epoxy Resins; D000431:Ethanol / Q000031:analogs & derivatives; D006358:Hot Temperature; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D004676:Myelin Basic Protein; D018993:Myelin P0 Protein; D009185:Myelin Proteins; D009419:Nerve Tissue Proteins / Q000032:analysis*; D016900:Neurofilament Proteins; D010525:Peripheral Nerves / Q000737:chemistry*
D000906:Antibodies; D004853:Epoxy Resins; D004676:Myelin Basic Protein; D018993:Myelin P0 Protein; D009185:Myelin Proteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C073687:PMP22 protein, human; C098088:sodium ethoxide; D000431:Ethanol