Homology, disruption and phenotypic analysis of CaGS Candida albicans gene induced during macrophage infection.
During macrophage infection Candida albicans expresses differentially several genes whose functions are associated with its survival strategy. Among others, we have isolated CaGS gene, which is homologous to SNF3, a glucose sensor of Saccharomyces cerevisiae. To elucidate its potential role during infection, CaGS has been disrupted and the resulting phenotype analyzed on different solid media. The null mutant lost the ability to form hyphae on a medium with low glucose concentration and serum. Furthermore, this mutant does not disrupt macrophage in in vitro infections. We believe that this putative glucose sensor is involved in hyphal development during macrophage infection.
Luongo
Marina
M
Laboratory of Molecular Fungal Pathogenesis, Institute of Genetics and Biophysics Buzzati Traverso, Naples, Italy.
Porta
Amalia
A
Maresca
Bruno
B
D002176:Candida albicans / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D002478:Cells, Cultured; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D014774:Virulence
D005656:Fungal Proteins; D009004:Monosaccharide Transport Proteins; C104463:SNF3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D005947:Glucose