[Influence of cultivation conditions on bacterial DNA content and bacterial membrane permeability].
The study included measurement of intracellular and extracellular DNA content and evaluation of bacterial membrane condition during plankton growth and in isolated communities. This was performed at different stages of bacterial development and after heating at the temperature of heat shock system activation. The content of intracellular bacterial DNA in the community decreased after 72 hours of cultivation growth more prominently than during plankton growth. Change of intracellular bacterial DNA content in the community overtook the increase of membrane permeability, which may indicate the onset of apoptosis. Heating at 420' decreased the permeability of bacterial membranes in the community, but did not affect this parameter in the bacteria during plankton growth. Extracellular DNA was found in the matrix of the communities after 24 hours of growth. Appearance of this DNA after 48-74 hours of cultivation may be associated with apoptotic process.
Tets
V V
VV
Ivanov
S D
SD
Tets
G V
GV
Pisareva
M M
MM
Iamshanov
V A
VA
D001419:Bacteria / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis* / Q000187:drug effects; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism
D003470:Culture Media; D004269:DNA, Bacterial