Identification of quantitative trait loci for susceptibility to mouse adenovirus type 1.
Adult SJL/J mice are highly susceptible to mouse adenovirus type 1 (MAV-1) infections, whereas other inbred strains, including BALB/cJ, are resistant (K. R. Spindler, L. Fang, M. L. Moore, C. C. Brown, G. N. Hirsch, and A. K. Kajon, J. Virol. 75:12039-12046, 2001). Using congenic mouse strains, we showed that the H-2(s) haplotype of SJL/J mice is not associated with susceptibility to MAV-1. Susceptibility of MAV-1-infected (BALB/cJ x SJL/J)F(1) mice was intermediate between that of SJL/J mice and that of BALB/cJ mice, indicating that susceptibility is a genetically controlled quantitative trait. We mapped genetic loci involved in mouse susceptibility to MAV-1 by analysis of 192 backcross progeny in a genome scan with 65 simple sequence length polymorphic markers. A major quantitative trait locus (QTL) was detected on chromosome 15 (Chr 15) with a highly significant logarithm of odds score of 21. The locus on Chr 15 alone accounts for 40% of the total trait variance between susceptible and resistant strains. QTL modeling of the data indicated that there are a number of other QTLs with small effects that together with the major QTL on Chr 15 account for 54% of the trait variance. Identification of the major QTL is the first step in characterizing host genes involved in susceptibility to MAV-1.
Welton
Amanda R
AR
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, 48109-0620, USA.
Chesler
Elissa J
EJ
Sturkie
Carla
C
Jackson
Anne U
AU
Hirsch
Gwen N
GN
Spindler
Katherine R
KR
D000256:Adenoviridae; D000257:Adenoviridae Infections / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006183:H-2 Antigens / Q000235:genetics; D051379:Mice; D020297:Mice, Congenic / Q000821:virology; D040641:Quantitative Trait Loci
D006183:H-2 Antigens