[Study on the inhibitory effects of juice of tomato on the growth of human prostate carcinoma PC-3 cells].
OBJECTIVE
To study on the inhibitory effects of juice of tomato on the growth of human prostate carcinoma PC-3 cells and its possible mechanism.


METHODS
PC-3 cells were treated with the juice of tomato in different concentration (40, 80, 120 ul/ml) for 48h; the proliferation of PC-3 cells were measured by MTT assay, the comet assay was used to measure the DNA damage of PC-3 cells.


RESULTS
Juice of tomato could inhibit the proliferation of PC-3 cells, the growth inhibitory rate of experimental groups were significantly higher than that of control group, with very statistical significance; and it could induce the breakage of DNA single strand of PC-3 cells and resulted in comet cells with tail, Rate of DNA tail and the tail length of DNA increased with the increasing of concentration of juice of tomato, showing the obvious dose effect relationship.


CONCLUSIONS
Juice of tomato could lead to DNA damage of PC-3 cells, it was related to that could inhibit the proliferation of PC-3 cells.
Luo
Qiong
Q
School of Public Health, Wuhan University, Wuhan 430071, China.
Pang
Ya-Qin
YQ
Li
Zhuo-Neng
ZN
Yan
Jun
J
Yang
Mingguang
M
Zeng
Xiulin
X
D001628:Beverages; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D018551:Solanum lycopersicum / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000473:pathology*
D010936:Plant Extracts