title: Effect of various antagonists on the Channa striatus fillet extract antinociception in mice.
abstract: The effects of an aqueous supernatant of haruan (ASH) (Channa striatus) fillet extract on various antinociception receptor system activities were examined using a mouse abdominal-constriction model. Mice that were pretreated with distilled water, s.c., followed 10 min later by administration of 25%, 50%, and 100% concentration ASH, s.c., produced a significant concentration-dependent antinociceptive activity (p < 0.001). Pretreatment with naloxone (0.3, 1.0, and 3.0 mg/kg body mass), 10 min before ASH administration, failed to block the extract antinociception. Pretreatment of the 100% concentration ASH with mecamylamine (5 mg/kg), pindolol (10 mg/kg), and haloperidol (1 mg/kg) also did not cause any significant change in its antinociception. However, pretreatment with atropine (5 mg/kg), bicuculline (10 mg/kg), phenoxybenzamine (10 mg/kg), and methysergide (5 mg/kg) were found to reverse ASH antinociception. Based on the above findings, the ASH is suggested to contain different types of bioactive compounds that act synergistically on muscarinic, GABAA, alpha-adrenergic, and serotonergic receptor systems to produce the observed antinociception.
authors.affiliation: Department of Biomedical Sciences, Faculty of Medical and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. shaza8174@hotmail.com
authors.forename: Z A
authors.initials: ZA
authors.lastname: Zakaria
authors.forename: M R
authors.initials: MR
authors.lastname: Sulaiman
authors.forename: A M
authors.initials: AM
authors.lastname: Mat Jais
authors.forename: M N
authors.initials: MN
authors.lastname: Somchit
mesh_terms: D000700:Analgesics; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009119:Muscle Contraction / Q000187:drug effects; D018482:Muscle, Skeletal / Q000502:physiology*; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010147:Pain Measurement / Q000187:drug effects; D010473:Perciformes / Q000502:physiology*; D011930:Reaction Time / Q000187:drug effects; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors; D014020:Tissue Extracts / Q000037:antagonists & inhibitors* / Q000502:physiology*
chemical_list: D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D009292:Narcotic Antagonists; D011956:Receptors, Cell Surface; D014020:Tissue Extracts; D009270:Naloxone; D001241:Aspirin