Monitoring "promiscuous" drug effects on single cells of multiple cell types.
Recent advances in genomics and molecular pathogenesis studies have determined that many diseases are caused by a multiplicity of factors. New drug regimens may consist of multiple biologically active agents designed to act synergistically on multiple biochemical targets. Live cell assays are becoming a standard for identifying new drug candidates with an emphasis on "homogeneous" living cell assays in which multiple cell lines are mixed and monitored simultaneously. In this study, we used a high-density single living cell array, based on an optical imaging fiber bundle microwell array, to simultaneously monitor "promiscuous" drug effects on single cells of multiple cell types. Such a capability allows for a more comprehensive understanding of how cells dynamically respond to combinatorial drug libraries or how different cellular pathways and regulation circuits respond cooperatively to drugs in individual cells.
Kuang
Yina
Y
Department of Chemistry, Tufts University, Medford, MA 02155, USA.
Walt
David R
DR
D020650:Combinatorial Chemistry Techniques; D004249:DNA Damage; D004353:Drug Evaluation, Preclinical; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D005336:Fiber Optic Technology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D023281:Genomics; D049452:Green Fluorescent Proteins / Q000235:genetics; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D007700:Kinetics; D007763:Lac Operon / Q000235:genetics; D046228:Microarray Analysis; D016685:Mitomycin / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D011938:Rec A Recombinases / Q000235:genetics
C485182:Cyan Fluorescent Protein; D009153:Mutagens; D049452:Green Fluorescent Proteins; D007544:Isopropyl Thiogalactoside; D016685:Mitomycin; D011938:Rec A Recombinases