title: Biphenyl uptake by psychrotolerant Pseudomonas sp. strain Cam-1 and mesophilic Burkholderia sp. strain LB400.
abstract: We investigated the uptake of biphenyl by the psychrotolerant, polychlorinated biphenyl (PCB)-degrader, Pseudomonas sp. strain Cam-1 and the mesophilic PCB-degrader, Burkholderia sp. strain LB400. The effects of growth substrates, metabolic inhibitors, and temperature on [14C]biphenyl uptake were studied. Biphenyl uptake by both strains was induced by growth on biphenyl, and was inhibited by dinitrophenol (DNP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), which are metabolic uncouplers. The Vmax and Km for biphenyl uptake by Cam-1 at 22 degrees C were 5.4 +/- 1.7 nmol x min(-1) x (mg of cell protein)(-1) and 83.1 +/- 15.9 micromol x L(-1), respectively. The Vmax and Km for biphenyl uptake by LB400 at 22 degrees C were 3.2 +/- 0.3 nmol x min(-1) x (mg of cell protein(-1)) and 51.5 +/- 9.6 micromol x L(-1), respectively. At 15 degrees C, the maximum rate for biphenyl uptake by Cam-1 and LB400 was 3.1 +/- 0.3 nmol x min(-1) x (mg of cell protein)(-1) and 0.89 +/- 0.1 nmol x min(-1) x (mg of cell protein)(-1), respectively. Thus, the maximum rate for biphenyl uptake by Cam-1 at 15 degrees C was more than 3 times higher than that for LB400.
authors.affiliation: Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
authors.forename: Emma R
authors.initials: ER
authors.lastname: Master
authors.forename: James J
authors.initials: JJ
authors.lastname: McKinlay
authors.forename: Gordon R
authors.initials: GR
authors.lastname: Stewart
authors.forename: William W
authors.initials: WW
authors.lastname: Mohn
mesh_terms: D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism*; D019117:Burkholderia / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D003080:Cold Temperature; D004140:Dinitrophenols / Q000494:pharmacology; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D001713:Biphenyl Compounds; D004140:Dinitrophenols; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D011078:Polychlorinated Biphenyls