title: Proteomic analysis of the outer membrane of Anabaena sp. strain PCC 7120.
abstract: Anabaena is a model to analyze the evolutionary development of plastids, cell differentiation, and the regulation of nitrogen fixation. Thereby, the outer membrane proteome is the place of sensing environmental differences and during plastid development, systems for intracellular communication had to be added to the proteome of this membrane. We present a protocol for the isolation of the outer membrane from Anabaena and the analysis of the proteome using different tools. 55 proteins were identified.
authors.affiliation: Department of Biology I, LMU Munich, Menzinger Strasse 67, D-80638 Münich, Germany.
authors.forename: Suncana
authors.initials: S
authors.lastname: Moslavac
authors.forename: Rolf
authors.initials: R
authors.lastname: Bredemeier
authors.forename: Oliver
authors.initials: O
authors.lastname: Mirus
authors.forename: Bernhard
authors.initials: B
authors.lastname: Granvogl
authors.forename: Lutz A
authors.initials: LA
authors.lastname: Eichacker
authors.forename: Enrico
authors.initials: E
authors.lastname: Schleiff
mesh_terms: D017033:Anabaena / Q000166:cytology*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000302:isolation & purification*; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry*; D019295:Computational Biology; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D020543:Proteome