Antioxidative and antigenotoxic effects of Japanese horse chestnut (Aesculus turbinata) seeds.
Japanese horse chestnut seed extract (HCSE) dose-dependently inhibited the autooxidation of linoleic acid (IC(50): 0.2 mg/ml), and the inhibition was almost complete at a concentration of 1 mg/ml. The HCSE scavenged DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals and superoxide anions with EC(50)s of 0.65 and 0.21 mg/ml, respectively. However, it had no effect on hydrogen peroxide. The HCSE inhibited the genotoxicities of furylfuramide, N-methyl-N-nitrosourea, methyl methanesulfonate, mitomycin C, 2-aminoanthracene and aflatoxin B1 at a concentration of 1 mg/ml or more. Total polyphenol content of the HCSE was 21 mg/g (13 mg/g-seeds). These results indicate that the Japanese horse chestnut seed is an antioxidative and antimutagenic botanical resource.
Sato
Itaru
I
Laboratories of Veterinary Public Health, Faculty of Agriculture, Iwate University, Japan.
Suzuki
Tadahiko
T
Kobayashi
Haruo
H
Tsuda
Shuji
S
D031319:Aesculus / Q000737:chemistry*; D016604:Aflatoxin B1 / Q000378:metabolism; D000873:Anthracenes / Q000378:metabolism; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005668:Furylfuramide / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D019787:Linoleic Acid / Q000037:antagonists & inhibitors; D008741:Methyl Methanesulfonate / Q000378:metabolism; D008770:Methylnitrosourea / Q000378:metabolism; D016685:Mitomycin / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*
D000873:Anthracenes; D016587:Antimutagenic Agents; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D005668:Furylfuramide; D016685:Mitomycin; D008770:Methylnitrosourea; C006593:2-anthramine; D019787:Linoleic Acid; D016604:Aflatoxin B1; D008741:Methyl Methanesulfonate; D006861:Hydrogen Peroxide