Morphological analysis of cell subpopulations within carotid atherosclerotic plaques.
Atherosclerosis is considered a chronic inflammatory process, prompted by lipid accumulation and propagated by cell-mediated mechanisms. The present work was undertaken to clarify this process by characterizing cellular components of inflammatory infiltrate localized within atheroma. Cryostat sections of atherosclerotic lesions obtained from human carotid endarterectomy were analysed immunohistochemically by using monoclonal and polyclonal antibody directed against T cell subpopulations (CD3, CD4, CD8), B cells (CD20), plasma cells (CD138), macrophages (CD14), mast cells (anti-tryptase). Our results assess that T cells are the predominant cell type among plaque infiltrating inflammatory cells. B cells were detected near the lipid core of atheroma and clusters of plasma cells were observed within cellular infiltrates in most plaques. Numerous tryptase positive mast cells were noticed in many areas of complicated lesions. Our results indicate the presence of many inflammatory cells within type V and VI atherosclerotic plaques, suggesting the involvement of those cells in plaque progression. In fact it was previously shown that stability of atherosclerotic lesions is influenced by mast cell-released matrix metalloproteinases which induce plaque rupture and by cytokines and chemokines which increase local inflammatory response and are produced by lymphocytes and macrophages.
Businaro
R
R
Department of Cardiovascular Sciences, University of Rome La Sapienza, Rome, Italy. rita.businaro@uniroma1.it
Digregorio
M
M
Riganò
R
R
Profumo
E
E
Buttari
B
B
Leone
S
S
Salvati
B
B
Capoano
R
R
D'Amati
G
G
Fumagalli
L
L
D000368:Aged; D000369:Aged, 80 and over; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D002340:Carotid Artery Diseases / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D016893:Carotid Stenosis / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000276:immunology / Q000473:pathology*; D018836:Inflammation Mediators / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology*; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D008407:Mast Cells / Q000276:immunology / Q000473:pathology; D008875:Middle Aged; D010950:Plasma Cells / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
D000954:Antigens, Surface; D018836:Inflammation Mediators