title: Do serum levels of eosinophil granule-derived protein change in patients undergoing pelvic radiotherapy?
abstract: OBJECTIVE
  Eosinophils have an important role in the pathogenesis of inflammatory bowel disease, with faecal levels of the eosinophil granule proteins, eosinophil cationic protein (ECP) and eosinophil protein X (EPX) reflecting disease activity. Eosinophil crypt abscesses are a characteristic histological finding in acute gastrointestinal radiation-induced mucosal damage. This pilot study aimed to investigate changes in serum levels of ECP/EPX during pelvic radiotherapy.
  
  
  METHODS
  Patients with no history of inflammatory bowel disease, starting a 5-week course of pelvic radiotherapy, had serum ECP/EPX levels measured before radiotherapy and during the fourth week of treatment. Bowel toxicity was graded at week 4 using the Common Toxicity Criteria Scale.
  
  
  RESULTS
  Fifteen patients who were to undergo adjuvant radiotherapy for gynaecological cancer were recruited. The mean serum levels of ECP and EPX before treatment were 17.3 microg/l (range 2.0-49.3 microg/l) and 37.3 microg/l (range 12.0-94.0 microg/l), respectively. The mean serum levels during week 4 of radiotherapy for ECP and EPX were 43.0 microg/l (range 2.4-164.0 microg/l) and 38.7 microg/l (range 9.0-79.0 microg/l), respectively. Serum ECP levels increased at week 4 compared with levels before radiotherapy (P = 0.02). Acute bowel toxicity was seen in 12 patients (80%) at week 4: Grade 1 in 25% patients and Grade 2 in 75%. In this small study, no correlation was seen between acute bowel toxicity at week 4 and serum ECP or EPX levels.
  
  
  CONCLUSIONS
  Serum ECP levels increase in response to pelvic irradiation. This may reflect the known involvement of eosinophils in the acute response to radiotherapy. Further study is required to determine when levels start to rise and their relationship to the degree of acute bowel toxicity.
authors.affiliation: Department of Oncology, East Gloucestershire NHS Trust, Cheltenham, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Bowen
authors.forename: K
authors.initials: K
authors.lastname: Newbold
authors.forename: P
authors.initials: P
authors.lastname: Blake
authors.forename: G
authors.initials: G
authors.lastname: Wild
authors.forename: W
authors.initials: W
authors.lastname: Egner
authors.forename: A R
authors.initials: AR
authors.lastname: Norman
authors.forename: H J N
authors.initials: HJ
authors.lastname: Andreyev
mesh_terms: D000368:Aged; D047091:Eosinophil Granule Proteins / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D005260:Female; D005833:Genital Neoplasms, Female / Q000532:radiotherapy; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood; D008875:Middle Aged; D010388:Pelvis / Q000528:radiation effects*; D010865:Pilot Projects; D018714:Radiotherapy, Adjuvant / Q000009:adverse effects*; D013997:Time Factors
chemical_list: D047091:Eosinophil Granule Proteins