title: Triacylglycerol oxidation in pig lipoproteins after a diet rich in oxidized sunflower seed oil.
abstract: The effects of two sunflower seed oil diets differing in oxidation levels (PV in oils 1 and 190 mequiv O2/kg) on lipoprotein TAG and total lipid oxidation were investigated in growing pigs. For 2 wk, two groups of 10 pigs were fed either of the diets, after which blood samples were collected. A method based on RP-HPLC and electrospray ionization-MS was used for the analysis of oxidized TAG molecules in chylomicrons and VLDL. The baseline diene conjugation method was used for the estimation of in vivo levels of lipoprotein lipid oxidation. TAG molecules with a hydroxy, an epoxy, or a keto group attached to a FA, as well as TAG core aldehydes were detected in the samples. Typically, lipoprotein TAG and total lipids were more oxidized in the pigs fed on the oxidized oil compared with those fed on nonoxidized oil. Oxidation of dietary fat was thus reflected in the lipoprotein oxidation, which confirmed our earlier findings.
authors.affiliation: Department of Biochemistry and Food Chemistry, University of Turku, FI-20014 Turku, Finland. jusuom@utu.fi
authors.forename: Jukka-Pekka
authors.initials: JP
authors.lastname: Suomela
authors.forename: Markku
authors.initials: M
authors.lastname: Ahotupa
authors.forename: Heikki
authors.initials: H
authors.lastname: Kallio
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002914:Chylomicrons / Q000032:analysis; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D008074:Lipoproteins / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000737:chemistry; D008079:Lipoproteins, VLDL / Q000737:chemistry; D008297:Male; D010084:Oxidation-Reduction; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D000074242:Sunflower Oil; D013552:Swine; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D002914:Chylomicrons; D004041:Dietary Fats; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D010938:Plant Oils; D000074242:Sunflower Oil; D014280:Triglycerides