Listeria monocytogenes ferritin protects against multiple stresses and is required for virulence.
In this study, the role of Listeria monocytogenes ferritin was investigated. The fri gene encoding the ferritin was deleted and the phenotype of the mutant was analyzed demonstrating that ferritin is necessary for optimal growth in minimal medium in both presence and absence of iron, as well as after cold- and heat-shock. We also showed that ferritin provides protection against reactive oxygen species and is essential for full virulence of L. monocytogenes. A comparative proteomic analysis revealed an effect of the fri deletion on the levels of listeriolysin O and several stress proteins. Together, our study demonstrates that fri has multiple roles that contribute to Listeria virulence.
Dussurget
Olivier
O
Unité des Interactions Bactéries-Cellules, INSERM U604, INRA USC2020, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France.
Dumas
Emilie
E
Archambaud
Cristel
C
Chafsey
Ingrid
I
Chambon
Christophe
C
Hébraud
Michel
M
Cossart
Pascale
P
D000222:Adaptation, Physiological; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D001427:Bacterial Toxins / Q000032:analysis; D004195:Disease Models, Animal; D005293:Ferritins / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D006360:Heat-Shock Proteins / Q000032:analysis; D006460:Hemolysin Proteins; D008089:Listeria monocytogenes / Q000254:growth & development / Q000472:pathogenicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D020543:Proteome / Q000032:analysis; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000502:physiology
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D020543:Proteome; D037521:Virulence Factors; D005293:Ferritins; C038705:hlyA protein, Listeria monocytogenes