[Improvement of restoration of heart function after ischemia by modified reperfusion solution].
OBJECTIVE
To elucidate effect of saline reperfusion solution (RS) containing aspartic acid, glucose, mannit, and trisamine (pH 7.5+/-0.1 at 22 degrees C) on restoration of isolated perfused rat heart function.


METHODS
After 20 minutes of initial perfusion hearts were sequentially subjected to 5 min of cardioplegia, 40 min of normothermic total ischemia and 30-min reperfusion. Hearts of groups 1 and 2 were stopped by hyper-potassium cardioplegic solution and after ischemia reperfused retrogradely for 5 min at 22 degrees C with Krebs solution and RS, respectively. This was followed by antegrade reperfusion with Krebs solution for 25 min at 37 degrees C. In groups 3 and 4 cardioplegia was instituted by perfusol while reperfusion was carried out in the same way as in groups 1 and 2.


RESULTS
RS significantly improved restoration of contractile and pump functions. Action of RS was more effective when it was used concomitantly with Perfusol. Thus by the end of reperfusion product of heart rate and left ventricular developed pressure was 76+/-7 and 56+/-2% of initial value in groups 4 and 3, respectively (p<0.05). External heart work calculated as product of cardiac output and mean aortic pressure was restored up to 72+/-4 and 35+/-3% of initial value in groups 4 and 3, respectively (p<0.001).


CONCLUSIONS
RS containing anaerobically utilizable substrates (aspartic acid, glucose), cellular membrane protectors, and antioxidants (mannit and trisamine), as well as optimal mixture of inorganic salts, significantly reduced damaging action of early reperfusion caused by activation of lipid peroxidation, Ca2+ overload and derangements of oxidative phosphorylation.
Pisarenko
O I
OI
Shul'zhnko
V S
VS
Studneva
I M
IM
D000818:Animals; D002302:Cardiac Output; D002314:Cardioplegic Solutions; D006324:Heart Arrest, Induced / Q000379:methods*; D006339:Heart Rate; D008297:Male; D017202:Myocardial Ischemia / Q000503:physiopathology; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D012996:Solutions; D013997:Time Factors
D002314:Cardioplegic Solutions; D012996:Solutions