title: Comparison of strand displacement and ligase chain amplification for detection of Chlamydia trachomatis infection in urogenital specimens.
abstract: Two amplification tests for the diagnosis of Chlamydia trachomatis infection, namely the ligase chain reaction (LCx) and the strand displacement assay (ProbeTec), were compared using samples from 1183 patients at sexually transmitted disease clinics. The overall prevalence of positive results was 8.0%, with agreement between the two assays of 98.8%. For endocervical, urethral and male urine samples, agreement was 99.3%, 99.4% and 97.7%, respectively. For ten discrepant samples, alternative amplification assays suggested that the LCx and ProbeTec assays gave erroneous results in six and four cases, respectively. Inhibition of amplification was observed with three (0.25%) urine specimens.
authors.affiliation: Department of Clinical Microbiology and Immunology, Lund University Hospital, Lund, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Zeeberg
authors.forename: H
authors.initials: H
authors.lastname: Miörner
authors.forename: I
authors.initials: I
authors.lastname: Thelin
authors.forename: S
authors.initials: S
authors.lastname: Agren
authors.forename: C
authors.initials: C
authors.lastname: Schalén
mesh_terms: D002584:Cervix Uteri / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis; D005260:Female; D052776:Female Urogenital Diseases / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D022521:Ligase Chain Reaction / Q000379:methods; D008297:Male; D052801:Male Urogenital Diseases; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010957:Plasmids; D013548:Sweden; D014521:Urethra / Q000382:microbiology; D014556:Urine / Q000382:microbiology