OpsX from Haemophilus influenzae represents a novel type of heptosyltransferase I in lipopolysaccharide biosynthesis.
The inner core region of the lipopolysaccharide (LPS) of Haemophilus influenzae is characterized by the presence of a phosphorylated 3-deoxy-alpha-D-manno-octulosonic acid (Kdo). In this study, we show that the heptosyltransferase I adding the first L-glycero-D-manno-heptose residue to this acceptor is encoded by the gene opsX, which differs in substrate specificity from the other heptosyltransferase I, known as WaaC.
Gronow
Sabine
S
Division of Medical and Biochemical Microbiology, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 22, D-23845 Borstel, Germany. sgronow@fz-borstel.de
Brabetz
Werner
W
Lindner
Buko
B
Brade
Helmut
H
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016695:Glycosyltransferases / Q000378:metabolism*; D006193:Haemophilus influenzae / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D013058:Mass Spectrometry; D010957:Plasmids; D013379:Substrate Specificity
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D016695:Glycosyltransferases; C504964:OpsX protein, Haemophilus influenzae