Effect of cytokines on ICAM-1 and ZO-1 expression on human airway epithelial cells.
The presence of adhesion molecules on airway epithelial cells may be important in recruiting leukocytes to the epithelium. The study aimed at investigating the effects of interleukin (IL)-4, IL-8, IL-13 and interferon-gamma (IFN-gamma) on cell viability and intracellular adhesion molecule (ICAM)-1 and zonula occludens protein (ZO)-1 expression on cultured human basal and columnar airway epithelial cells. Cycloheximide (CHX) induced cell death in both cell lines. The cytokines IL-4, IL-8, IL-13 and IFN-gamma had only minor effects on cell proliferation in the columnar 16HBE14o-cells, and inhibited the effects of CHX on cell death. IFN-gamma increased ICAM-1 expression in both cell lines. Western blot analysis showed that CHX inhibited both ICAM-1 and ZO-1 expression in the basal cell line. A combination of IL-4 and IFN-gamma appeared to break the tight junctions. IL-4 and IL-13 potentiated CHX-induced apoptosis in basal cells but not in columnar cells, possibly due to low levels of the IL-4 receptor. It is concluded that cytokines produced by airway epithelium may have a role in regulating sequestering of leukocytes to the airways during airway inflammation.
Relova
Anne-Jacqueline
AJ
Department of Medical Cell Biology, University of Uppsala, Box 571, SE-75 123 Uppsala, Sweden.
Shahana
Shahida
S
Makeeva
Natalia
N
Roomans
Godfried M
GM
D001980:Bronchi / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D016207:Cytokines / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000276:immunology; D007371:Interferon-gamma / Q000502:physiology; D018793:Interleukin-13 / Q000502:physiology; D015847:Interleukin-4 / Q000502:physiology; D007962:Leukocytes / Q000502:physiology; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000276:immunology; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000276:immunology; D019948:Receptors, Interleukin-4 / Q000096:biosynthesis; D020545:Respiratory Mucosa / Q000276:immunology / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D019108:Tight Junctions / Q000502:physiology / Q000648:ultrastructure; D062826:Zonula Occludens-1 Protein
D016207:Cytokines; D018793:Interleukin-13; D008565:Membrane Proteins; D010750:Phosphoproteins; D019948:Receptors, Interleukin-4; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D018799:Intercellular Adhesion Molecule-1; D015847:Interleukin-4; D007371:Interferon-gamma