title: p27(kip1) and Ki-67 (MIB1) immunohistochemical expression in radical prostatectomy specimens of patients with clinically localized prostate cancer.
abstract: The immunohistochemical expressions (IE) of p27(kip1) and Ki-67 (MIB-1), both involved in cell cycle regulation and cell proliferation, and their ability to predict biochemical failure, were assessed in patients with clinically localized prostate cancer who had underdone radical prostatectomy of curative intent. In addition, p27(kip1) and Ki-67 (MIB1) expressions were correlated with several pre-operative and post-operative parameters, such as Gleason score, extracapsular extension, seminal vesicle involvement, pelvic lymph nodes metastasis, positive surgical margins, coexistence of high-grade prostatic intraepithelial neoplasia, tumour size, prostate volume and PSA levels. Our analysis involved 130 consecutive radical prostatectomy specimens. A statistically significant correlation of low p27(kiP1) IE with seminal vesicles involvement, increased tumour volume and high pre-operative PSA values was documented. Low p27(kiP1) IE was significantly correlated with an increased likelihood of biochemical failure after radical prostatectomy. In addition, the increased IE of Ki-67 (MIB1) correlated significantly with metastatic disease in the pelvic lymph nodes and was a significant predictor of biochemical failure. Cox regression analysis, which included p27(kip1) expression, Ki-67 (MIB1) expression and all the pre-operative and post-operative parameters, showed that pelvic lymph node involvement and Ki-67 (MIB1) IE were independent prognostic markers of biochemical failure after radical prostatectomy.
authors.affiliation: Department of Pathology, "Evangelismos" General Hospital, Athens, Greece.
authors.forename: Kyriakos
authors.initials: K
authors.lastname: Revelos
authors.forename: Constantina
authors.initials: C
authors.lastname: Petraki
authors.forename: Alkiviadis
authors.initials: A
authors.lastname: Gregorakis
authors.forename: Andreas
authors.initials: A
authors.lastname: Scorilas
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Papanastasiou
authors.forename: Roxane
authors.initials: R
authors.lastname: Tenta
authors.forename: Michael
authors.initials: M
authors.lastname: Koutsilieris
mesh_terms: D000368:Aged; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000096:biosynthesis*; D049109:Cell Proliferation; D050760:Cyclin-Dependent Kinase Inhibitor p27; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009362:Neoplasm Metastasis; D011379:Prognosis; D016016:Proportional Hazards Models; D011467:Prostate / Q000473:pathology; D017430:Prostate-Specific Antigen / Q000096:biosynthesis; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000601:surgery*; D012044:Regression Analysis; D013997:Time Factors; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis*
chemical_list: D018797:Cell Cycle Proteins; D019394:Ki-67 Antigen; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D017430:Prostate-Specific Antigen