title: Preventing hepatocyte oxidative stress cytotoxicity with Mangifera indica L. extract (Vimang).
abstract: Vimang is an aqueous extract of Mangifera indica used in Cuba to improve the quality of life in patients suffering from inflammatory diseases. In the present study we evaluated the effects of Vimang at preventing reactive oxygen species (ROS) formation and lipid peroxidation in intact isolated rat hepatocytes. Vimang at 20, 50 and 100 microg/ml inhibited hepatocyte ROS formation induced by glucose-glucose oxidase. Hepatocyte cytotoxicity and lipid peroxidation induced by cumene hydroperoxide was also inhibited by Vimang in a dose and time dependent manner at the same concentration. Vimang also inhibited superoxide radical formation by xanthine oxidase and hypoxanthine. The superoxide radical scavenging and antioxidant activity of the Vimang extract was likely related to its gallates, catechins and mangiferin content. To our knowledge, this is the first report of cytoprotective antioxidant effects of Vimang in cellular oxidative stress models.
authors.affiliation: Centro Nacional para el Control Estatal de la Calidad de los Medicamentos, Playa, Habana, Cuba.
authors.forename: Diadelis
authors.initials: D
authors.lastname: Remirez
authors.forename: Shahrzad
authors.initials: S
authors.lastname: Tafazoli
authors.forename: Rene
authors.initials: R
authors.lastname: Delgado
authors.forename: Asghar A
authors.initials: AA
authors.lastname: Harandi
authors.forename: Peter J
authors.initials: PJ
authors.lastname: O'Brien
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001555:Benzene Derivatives / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005707:Gallic Acid / Q000494:pharmacology; D005949:Glucose Oxidase / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D019271:Hypoxanthine / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D031022:Mangifera; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011435:Propyl Gallate / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001555:Benzene Derivatives; C501277:Mangifera indica extract; D016877:Oxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013481:Superoxides; D019271:Hypoxanthine; D005707:Gallic Acid; D011435:Propyl Gallate; D005949:Glucose Oxidase; D014969:Xanthine Oxidase; C007164:cumene hydroperoxide