title: Histologic evaluation of the effect of nicotine administration on bone regeneration. A study in dogs.
abstract: The objective of this study was to investigate the histometric impact of nicotine on bone regeneration of surgically created alveolar ridge defects in dogs. Sixteen mongrel dogs were used. One defect was surgically created unilaterally in the mandible, and left to heal spontaneously. The animals were randomly assigned to one of the following groups: Group 1--control (n = 8) and Group 2--subcutaneous nicotine administration (2 mg/kg) twice a day (n = 8). After 4 months, the animals were sacrificed and the specimens routinely processed for semi-serial decalcified sections. Bone height (BH), bone width (BW), bone density (BD), and bone area (BA) of the newly-formed bone were evaluated. Intergroup analysis (Mann-Whitney rank sum test) showed that regardless of the presence of nicotine, no significant differences were observed regarding bone width (BW), bone area (BA) and bone height (BH) (p > 0.05). On the other hand, it was demonstrated that nicotine administration significantly influenced the proportion of mineralized tissue within the limits of the newly-formed bone (BD) (p < 0.001). Within the limits of the present study, it can be concluded that nicotine might affect but not prevent bone healing in defects left to heal spontaneously.
authors.affiliation: Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry of Piracicaba, State University of Campinas.
authors.forename: Juliana Bezerra
authors.initials: JB
authors.lastname: Saldanha
authors.forename: Suzana Peres
authors.initials: SP
authors.lastname: Pimentel
authors.forename: Marcio Zaffalon
authors.initials: MZ
authors.lastname: Casati
authors.forename: Antonio Wilson
authors.initials: AW
authors.lastname: Sallum
authors.forename: Enilson Antonio
authors.initials: EA
authors.lastname: Sallum
authors.forename: Francisco Humberto Júnior
authors.initials: FH
authors.lastname: Nociti
mesh_terms: D000539:Alveolar Process / Q000187:drug effects* / Q000601:surgery; D000541:Alveolectomy; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D003367:Cotinine / Q000097:blood; D004285:Dogs; D023421:Models, Animal; D009538:Nicotine / Q000097:blood / Q000494:pharmacology*; D011897:Random Allocation; D018709:Statistics, Nonparametric; D014945:Wound Healing / Q000187:drug effects
chemical_list: D009538:Nicotine; D003367:Cotinine