Promotion of long-term heart allograft survival by combination of mobilized donor plasmacytoid dendritic cells and anti-CD154 monoclonal antibody.
Infusion of donor immature dendritic cells (DC) can significantly prolong survival of organ allografts, and this is believed to be due to antigen recognition by T cells in the absence of co-stimulation. In this study we report that a single pre-operative infusion of donor-mobilized immature plasmacytoid dendritic cells (pDCs) is superior to that of other DC sub-sets in suppressing allograft rejection. The combination of pDC infusion with injection of anti-CD154 monoclonal antibody further inhibited graft rejection and, in 50% of the mice, led to indefinite graft survival. This finding suggests a role for the plasmacytoid DC sub-set in facilitating organ transplant survival and also in the treatment of autoimmune disorders.
Björck
P
P
Department of Dermatology, University of Pittsburgh, Pennsylvania, USA. bjorck@stanford.edu
Coates
P T H
PT
Wang
Z
Z
Duncan
F J
FJ
Thomson
A W
AW
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology*; D023201:CD40 Ligand / Q000494:pharmacology*; D003131:Combined Modality Therapy; D003713:Dendritic Cells / Q000637:transplantation*; D004195:Disease Models, Animal; D006084:Graft Rejection; D006085:Graft Survival; D016027:Heart Transplantation / Q000276:immunology* / Q000379:methods; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011336:Probability; D011897:Random Allocation; D012016:Reference Values; D012680:Sensitivity and Specificity; D014181:Transplantation Immunology / Q000187:drug effects* / Q000502:physiology; D014184:Transplantation, Homologous
D000888:Antibodies, Anti-Idiotypic; D023201:CD40 Ligand