The mechanism of lipopolysaccharide infiltration through HUVEC membrane surface in direct endotoxin injury.
In this study, the HUVEC's cellular biomechanical properties of HUVEC (elastic modulus K1, K2 and viscoefficient mu) were determined with micropipette aspiration system and analyzed after being directly damaged with lipopolysaccharide (LPS). The phospholipid compositions of HUVEC membrane were analyzed with high-performance capillary electrophoresis and PLA2 activity was determined to research the modification and metabolism of HUVEC membrane phospholipid. Infiltration of LPS on HUVEC membrane was studied by observation with confocal microscopy and fluorescent microscopy. Results showed that LPS direct injuring HUVEC can cause the changes of HUVEC biomechanical properties and membrane lipid contents; HUVEC directly damage by LPS could also activate HUVEC phospholipase A2 (PLA2), influencing membrane lipid metabolism; LPS could directly infiltrate and intercalate HUVEC membrane, causing and membrane contents variation. Based on these experimental results, the mechanism of lipopolysaccharide infiltration VEC membrane surface in direct LPS injury was studied and analyzed in view of the cellular biomechanical mechanism.
Wang
Xiang
X
College of Bioengineering, Key Laboratory for Biomechanics and Tissue Engineering under the State Ministry of Education, Chongqing University, Chongqing 400044, PR China. xwangchn@cqu.edu.cn
Cai
Shao-Xi
SX
Wang
Xiao-Jun
XJ
Luo
Xiang-Dong
XD
Yang
Zong-Cheng
ZC
D001696:Biomechanical Phenomena; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D019075:Electrophoresis, Capillary; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004731:Endotoxins / Q000494:pharmacology*; D004789:Enzyme Activation; D006801:Humans; D007700:Kinetics; D008070:Lipopolysaccharides / Q000378:metabolism* / Q000494:pharmacology; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology
D004731:Endotoxins; D008070:Lipopolysaccharides; D010741:Phospholipases A; D054467:Phospholipases A2