Induced accumulation of oleanolic acid and ursolic acid in cell suspension cultures of Uncaria tomentosa.
Increasing sucrose from 20 to 50 g l(-1) in Uncaria tomentosa cell suspension cultures enhanced ursolic acid and oleanolic acid production from 129 +/- 61 to 553 +/- 193 microg g(-1) cell dry wt. The maximal concentration of both triterpenes (1680 +/- 39 microg g(-1) cell dry wt) was 8 days after elicitation by jasmonic acid, while yeast extract or citrus pectin treatments produced 1189 +/- 20 or 1120 +/- 26 microg g(-1) cell dry wt, respectively. The ratio of ursolic acid:oleanolic acid was constant at 70:30.
Feria-Romero
Iris
I
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional. Av. Instituto Politécnico Nacional, México.
Lazo
Elizabeth
E
Ponce-Noyola
Teresa
T
Cerda-García-Rojas
Carlos M
CM
Ramos-Valdivia
Ana C
AC
D018533:Biomass; D020948:Cat's Claw / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000737:chemistry / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors; D014315:Triterpenes / Q000737:chemistry / Q000378:metabolism*; D000097245:Ursolic Acid
D009828:Oleanolic Acid; D013395:Sucrose; D014315:Triterpenes; D000097245:Ursolic Acid