The red wine polyphenol, resveratrol, exerts acute direct actions on guinea-pig ventricular myocytes.
Epidemiological evidence suggests that moderate consumption of red wine may be cardioprotective, although the precise mechanism(s) responsible remains poorly understood. We hypothesized that the red wine polyphenol, resveratrol, may exert direct actions on the heart and thus potentially contribute to cardioprotection. We show that resveratrol acutely decreases Ca2+ transient amplitude in isolated cardiac myocytes. Intriguingly, resveratrol simultaneously increases cell shortening in half the cells tested, while decreasing shortening in the other half. The former could be attributed to heightened myofilament Ca2+ sensitivity. This was no longer observed in myocytes that had been incubated with the oestrogen receptor antagonist, ICI 182,780, suggesting an oestrogen-receptor dependent mechanism of action. In addition, resveratrol significantly decreased action potential duration and the peak L-type Ca2+ current. Our findings provide evidence that resveratrol exerts multiple direct actions on cardiac myocytes, the net result of which is no overall change in cell contraction. The clinical significance of these results remains to be determined.
Liew
Reginald
R
Cardiac Medicine, National Heart and Lung Institute, Imperial College London, Dovehouse Street, London, SW3 6LY, United Kingdom.
Stagg
Mark A
MA
MacLeod
Kenneth T
KT
Collins
Peter
P
D000818:Animals; D002118:Calcium / Q000378:metabolism; D048429:Cell Size / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D000077267:Fulvestrant; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology; D059808:Polyphenols; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology; D016276:Ventricular Function; D014920:Wine
D004965:Estrogen Antagonists; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013267:Stilbenes; D014665:Vasodilator Agents; D000077267:Fulvestrant; D004958:Estradiol; D000077185:Resveratrol; D002118:Calcium