[Experimental study on preparation of decellularized artery vascular graft matrix and explantation of carotid artery allografts].
OBJECTIVE
To set up a new process to access the preparation of decellularized artery grafts. And to evaluate the feasibility of decellularized artery allografts was evaluated.


METHODS
This study compared the effects of four extraction chemicals [1% t-octyl-phenoxypolyethoxyethanol (Triton X-100), 1% tri (n-butyl) phosphate (TnBP), and 1% sodium dodecyl sulfate (SDS) and trypsin (0.125, 0.25%) on thoracic artery vascular for 24 h (except trypsin for 2 h). At the base of it, a four-step process, including hypotonic, hypertonic solutions and combining with 1% Triton X-100 and 1% SDS detergents, were performed in rabbit thoracic artery vascular. Histological examination, tensile tests and expanding-burst tests were done on the samples. The decellularized carotid artery allografts were transplanted in other rabbits.


RESULTS
Treatment with 1% SDS or 1% Triton X-100 for 24 h could remove most cells with retention of near normal structure. A four-step process could remove all cells with the extracellular matrix well conserved. The pulling mechanical properties and burst pressure of decellularized carotid artery were similar to the control. The decellularized carotid artery allografts (diameter of 2 mm) were patent at explanting up to 2 months.


CONCLUSIONS
The acellular artery vascular graft matrix is well prepared with four-step process including detergents, such as TritonX-100, SDS without compromising the graft structure or mechanical properties significantly. The carotid artery allografts (diameter of 2 mm) decellularized by the process are patent at explanting up to 2 months.
Fan
Heng-hua
HH
Department of Orthopaedic, Air Force General Hospital, Beijing 100036, China. fanhh8@yahoo.com.cn
Zhang
Bo-xun
BX
Liang
Xiang-dang
XD
Wang
Ai-yuan
AY
Zhao
Bin
B
Cui
Xue-mei
XM
Hu
Yi-yun
YY
Wu
Di
D
Zhou
Hua
H
Wang
Yan
Y
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D002339:Carotid Arteries / Q000166:cytology / Q000637:transplantation*; D005240:Feasibility Studies; D005260:Female; D008297:Male; D011480:Protease Inhibitors / Q000494:pharmacology; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*
D011480:Protease Inhibitors; D012967:Sodium Dodecyl Sulfate