title: Identification of nitric oxide donors by biomimetic HTS application.
abstract: Metalloporphyrin catalyzed biomimetic oxidation was used for the identification of nitric oxide (NO) donors with diverse chemical structure. Methodology was validated by testing known NO donors. Efficient automation of the test allowed us to investigate a subset of our corporate library. Several hits identified in this campaign were validated in both the chemical and also microsomal model that revealed all hits to be active in the biological system, as well. One of the hits showed comparable activity to V-PYRRO/NO, the prototypic liver selective NO donor.
authors.affiliation: Pilot Plant II, Gedeon Richter Ltd., P.O. Box 27, H-1475, Budapest, Hungary.
authors.forename: György T
authors.initials: GT
authors.lastname: Balogh
authors.forename: Balázs
authors.initials: B
authors.lastname: Dalmadi
authors.forename: Attila
authors.initials: A
authors.lastname: Bielik
authors.forename: György M
authors.initials: GM
authors.lastname: Keserü
mesh_terms: D000818:Animals; D032701:Biomimetics; D020650:Combinatorial Chemistry Techniques; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D008665:Metalloporphyrins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008862:Microsomes, Liver; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
chemical_list: D008665:Metalloporphyrins; D020030:Nitric Oxide Donors; D006861:Hydrogen Peroxide