Determination of testosterone concentrations in rat plasma using liquid chromatography-atmospheric pressure chemical ionization mass spectrometry combined with ethyl oxime and acetyl ester derivatization.
A quantitative method for measuring testosterone (T) concentrations in rat plasma was developed using ethyl oxime and acetyl ester derivatization and liquid chromatography-atmosphere pressure chemical ionization tandem mass spectrometry (LC-APCI-MS/MS). The method utilizes a solid phase extraction with Varian Bond Elut C18, a derivatization process to form testosterone ethoxime acetate and LC-APCI-MS/MS with a reversed phase LC and a C8 column. This method is capable of detecting testosterone concentrations as low as 0.2 ng/ml in a 0.05 ml sample of rat plasma. This method can be used as a sensitive chromatography-based assay for small sample volumes of rat blood.
Niwa
Makoto
M
Pharmacokinetics Group, Technical Development Department, Research and Development Division, Pharmaceuticals Group, Nippon Kayaku Co. Ltd., 3-31-12, Shimo, Kita-ku, Tokyo 115-8588, Japan. makoto.niwa@nipponkayaku.co.jp
Watanabe
Noriko
N
Ochiai
Hisashi
H
Yamashita
Kouwa
K
D000818:Animals; D001274:Atmospheric Pressure; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004952:Esters / Q000737:chemistry; D005260:Female; D008297:Male; D010091:Oximes / Q000737:chemistry; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013739:Testosterone / Q000097:blood* / Q000592:standards
D004952:Esters; D010091:Oximes; D013739:Testosterone