Biosynthetic (14)C-labelling of xanthohumol in hop cones.
Xanthohumol (Xn) has well-established chemopreventive potential in vitro. In order to carry out in vivo bioavailability and tissue distribution studies,( 14)C-labelled Xn was produced by biolabelling. Supplying hop sprouts with 5 mCi [U-(14)C]glucose led to incorporation of (14)C into Xn. Delivering the radioactive precursor at once resulted in Xn with a specific activity of 318 microCi.mmol(-1); if however the amount was supplied in aliquots over 4 days a specific activity of only 53.1 microCi.mmol(-1) Xn was obtained.
Berwanger
Stefanie
S
Pharmakognosie und Analytische Phytochemie, Universität des Saarlandes, Saarbrücken, Germany.
Frank
Norbert
N
Knauft
Jutta
J
Becker
Hans
H
D002250:Carbon Radioisotopes; D005419:Flavonoids; D005947:Glucose / Q000378:metabolism; D027582:Humulus / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods*; D011427:Propiophenones / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D027343:Rhizome / Q000378:metabolism
D002250:Carbon Radioisotopes; D005419:Flavonoids; D011427:Propiophenones; D005947:Glucose; C104536:xanthohumol