A general procedure for the production of antibody reagents against eukaryotic ribosomal proteins.
Despite recent progress in the structural and functional analysis of bacterial and archaeal ribosomes, the structure and biogenesis of eukaryotic ribosomes still awaits a detailed characterization. Ribosomal protein-specific antibodies would be valuable tools for such studies, but their production is commonly hindered by the poor expression and solubility of eukaryotic ribosomal proteins in E. coli. We report here an improved general procedure for the over-production of recombinant eukaryotic ribosomal proteins and for the generation of the corresponding polyclonal antibodies. The specificity and sensitivity of detection of the antibodies produced by this procedure are documented.
Dieci
Giorgio
G
Dipartimento di Biochimica e Biologia Molecolare, Università degli Studi di Parma, Parco Area delle Scienze 23/A, 43100 Parma, Italy. giorgio.dieci@unipr.it
Bottarelli
Lorena
L
Ottonello
Simone
S
D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000235:genetics; D005057:Eukaryotic Cells / Q000276:immunology / Q000378:metabolism*; D015151:Immunoblotting; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D012269:Ribosomal Proteins / Q000172:deficiency / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D011994:Recombinant Proteins; D012269:Ribosomal Proteins