title: Vacuolar H(+)-ATPase and plasma membrane H(+)-ATPase contribute to the tolerance against high-pressure carbon dioxide treatment in Saccharomyces cerevisiae.
abstract: As a non-thermal sterilization process, high-pressure carbon dioxide treatment (HPCT) is considered to be promising. The main sterilizing effect of HPCT is thought to be acidification in cytoplasm of microorganisms. We investigated the tolerance mechanism of Saccharomyces cerevisiae to HPCT with special reference to vacuolar and plasma membrane H(+)-ATPases. HPCT was imposed at 35 degrees C, 4 to 10 MPa, for 10 min. slp1 mutant defective in vacuole morphogenesis was more sensitive to HPCT than its isogenic parent. Concanamycin A, a specific inhibitor of vacuolar H(+)-ATPase (V-ATPase), at 10 microM rendered the parent more HPCT-sensitive to the level of slp1. To confirm further the contribution of V-ATPase to the tolerance against HPCT in S. cerevisiae, we compared vma1 mutant of V-ATPase with its isogenic parent for their HPCT sensitivity. vma1 mutant was more sensitive to HPCT than its parent. Addition of 10 microM vanadate, an inhibitor of plasma membrane H(+)-ATPase (P-ATPase), to the wild type strains also increased the inactivation ratio. These results clearly show that V- and P-ATPases contribute to the tolerance against HPCT in S. cerevisiae by accumulating excess H(+) from cytoplasm to vacuole and excluding H(+) outside of the cell, respectively.
authors.affiliation: Laboratory of Food Microbiology, Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866, Kameino, Fujisawa-shi, Kanagawa 252-8510, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Watanabe
authors.forename: Soichi
authors.initials: S
authors.lastname: Furukawa
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kitamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Takatsuki
authors.forename: Ryogo
authors.initials: R
authors.lastname: Hirata
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ogihara
authors.forename: Makari
authors.initials: M
authors.lastname: Yamasaki
mesh_terms: D002245:Carbon Dioxide / Q000494:pharmacology*; D005519:Food Preservation / Q000379:methods*; D005524:Food Technology; D018942:Macrolides / Q000494:pharmacology; D011312:Pressure; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D013696:Temperature; D013997:Time Factors; D025262:Vacuolar Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D014638:Vanadates / Q000494:pharmacology
chemical_list: D018942:Macrolides; D002245:Carbon Dioxide; D014638:Vanadates; C036978:concanamycin A; D025262:Vacuolar Proton-Translocating ATPases