Specificity of a Bacteroides thetaiotaomicron marker for human feces.
A bacterial primer set, known to produce a 542-bp amplicon specific for Bacteroides thetaiotaomicron, generated this product in PCR with 1 ng of extracted DNA from 92% of 25 human fecal samples, 100% of 20 sewage samples, and 16% of 31 dog fecal samples. The marker was not detected in 1 ng of fecal DNA from 61 cows, 35 horses, 44 pigs, 24 chickens, 29 turkeys, and 17 geese.
Carson
C Andrew
CA
Dept. of Veterinary Pathobiology, 201 Connaway Hall, University of Missouri, Columbia, MO 65211, USA. carsonc@missouri.edu
Christiansen
Jessica M
JM
Yampara-Iquise
Helen
H
Benson
Verel W
VW
Baffaut
Claire
C
Davis
Jerri V
JV
Broz
Robert R
RR
Kurtz
William B
WB
Rogers
Wendi M
WM
Fales
William H
WH
D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D001439:Bacteroides / Q000235:genetics*; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D004285:Dogs; D005243:Feces / Q000382:microbiology*; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D012680:Sensitivity and Specificity; D012722:Sewage / Q000382:microbiology; D014876:Water Pollution / Q000032:analysis*
D004269:DNA, Bacterial; D005819:Genetic Markers; D012722:Sewage