title: Fate redirection of hippocampal astrocytes toward neuronal lineage by aggregate culture.
abstract: Mammalian cells that have been committed to a certain cell lineage cannot be directed to other lineages. However, some astrocytes in the mammalian brains have been reported to represent plasticity to redirect to other cell lineages. We found that mouse hippocampal astrocytes cultured in aggregate forms of "astrosphere", redirected to MAP2-positive immature neurons. In astrospheres, basic HLH factors positively regulating neuronal differentiation were up-regulated and Id3 inhibiting basic HLH factors was down-regulated. Ectopic Id3 induction repressed redirection of astrocytes to a neuronal lineage, suggesting that astrosphere formation induced plasticity of astrocytes by changing the gene expression patterns.
authors.affiliation: Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan. myanagisawa@mail.mcg.edu
authors.forename: Makoto
authors.initials: M
authors.lastname: Yanagisawa
authors.forename: Kinichi
authors.initials: K
authors.lastname: Nakashima
authors.forename: Wataru
authors.initials: W
authors.lastname: Ochiai
authors.forename: Takumi
authors.initials: T
authors.lastname: Takizawa
authors.forename: Takao
authors.initials: T
authors.lastname: Setoguchi
authors.forename: Atsumi
authors.initials: A
authors.lastname: Uemura
authors.forename: Makiko
authors.initials: M
authors.lastname: Takizawa
authors.forename: Ikuo
authors.initials: I
authors.lastname: Nobuhisa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Taga
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D002449:Cell Aggregation; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology*; D007150:Immunohistochemistry; D051796:Inhibitor of Differentiation Proteins; D051379:Mice; D020932:Nerve Growth Factor / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D011506:Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051796:Inhibitor of Differentiation Proteins; D011506:Proteins; C067679:Idb3 protein, mouse; D020932:Nerve Growth Factor