Improved sensitivity of human immunodeficiency virus type 2 subtype B plasma viral load assay.
We developed a new assay for human immunodeficiency virus type 2 plasma RNA quantification based on a previous format. The new version performed significantly better than the original regarding the detection of subtype B, allowing the detection of 14 out of 36 plasma RNAs in the subtype B-infected patients not detected with the original version.
Damond
Florence
F
Laboratoire de Virologie, Hôpital Bichat Claude Bernard, 46 rue Henri Huchard, 75018 Paris, France. florence.damond@bch.ap-hop-paris.fr
Collin
Gilles
G
Descamps
Diane
D
Matheron
Sophie
S
Pueyo
Sophie
S
Taieb
Audrey
A
Campa
Pauline
P
Benard
Antoine
A
Chêne
Geneviève
G
Brun-Vezinet
Francoise
F
D001331:Automation; D015498:HIV-2 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D015203:Reproducibility of Results; D019562:Viral Load / Q000379:methods*
D012367:RNA, Viral