title: The upstream area of the chicken alpha-globin gene domain is transcribed in both directions in the same cells.
abstract: It was demonstrated previously that in erythroid chicken cells an extended upstream area of the alpha-globin gene domain is transcribed in both directions as a part of ggPRX gene and a part of a full domain transcript of the alpha-globin gene domain. Here, we show that both DNA chains of the above-mentioned region are transcribed in the same cells and that the corresponding transcripts coexist in nuclei. The data obtained suggest that cells possess a molecular mechanism which in some cases prevents the formation of dsRNA and subsequent destruction of both transcripts in spite of the presence of complementary RNA chains in the cell nucleus.
authors.affiliation: Laboratory of Structural and Functional Organization of Chromosomes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov Street, 119334 Moscow, Russia.
authors.forename: Victoria
authors.initials: V
authors.lastname: Borunova
authors.forename: Olga V
authors.initials: OV
authors.lastname: Iarovaia
authors.forename: Yegor S
authors.initials: YS
authors.lastname: Vassetzky
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Razin
mesh_terms: D000818:Animals; D002645:Chickens; D005914:Globins / Q000235:genetics*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012313:RNA / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D012313:RNA; D005914:Globins