[Effects of 2,5-hexanedione on NGF expression in motor and sensory neuronal cells of rats].
OBJECTIVE
To investigate the effects of 2,5-Hexanedione (2,5-HD), a metabolite of n-hexane,on nerve growth factor (NGF) expression in motor and sensory neuronal cells of peripheral nervous system of rats.


METHODS
Primary cultured dorsal root ganglion (DRG) neurons were obtained from new born rats on day 4. The cell line VSC4.1 were cultured. The growth condition of cells were observed with a phase contrast microscope. Immunohistochemistry technology was used to analyze the NGF expression in both VSC4.1 cells and primary cultured DRG cells treated with 4 different dosages of 2, 5-HD (2.5, 5.0, 10.0, 20.0 mmol/L) respectively for 24 hours.


RESULTS
The NGF expression decreased significantly in both DRG neurons and VSC4.1 cells dosed respectively with 5.0,10.0,20.0 mmol/L 2,5-HD in comparison with the control group (P < 0.05), with a tendency of dose-effect correlation.


CONCLUSIONS
The results suggested that 2,5-HD can decrease the endogenous NGF levels in both cultured DRG sensory neurons and VSC4.1 motor neuron cells of rats.
Liu
Qiang
Q
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Liu
Qing-Jun
QJ
Duan
Hua-Wei
HW
Zhang
Yan-Shu
YS
Le
Weidong
W
He
Fengsheng
F
Zheng
Yuxin
Y
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology*; D006588:Hexanones / Q000633:toxicity*; D009046:Motor Neurons / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011984:Sensory Receptor Cells / Q000378:metabolism*
D006588:Hexanones; D020932:Nerve Growth Factor; C011269:2,5-hexanedione