Multiplex approaches in protein microarray technology.
The success of genome sequencing projects has provided the basis for systematic analysis of protein function and has led to a shift from the description of single molecules to the characterization of complex samples. Such a task would not be possible without the provision of appropriate high-throughput technologies, such as protein microarray technology. In addition, the increasing number of samples necessitates the adaptation of such technologies to a multiplex format. This review will discuss protein microarray technology in the context of multiplex analysis and highlight its current prospects and limitations.
Kersten
Birgit
B
Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Strasse 10, D-13125 Berlin-Buch, Germany. b.kersten@mdc-berlin.de
Wanker
Erich E
EE
Hoheisel
Jörg D
JD
Angenendt
Philipp
P
D000818:Animals; D004247:DNA / Q000235:genetics; D006801:Humans; D007118:Immunoassay; D020411:Oligonucleotide Array Sequence Analysis; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D011506:Proteins; D004247:DNA