title: ProRule: a new database containing functional and structural information on PROSITE profiles.
abstract: BACKGROUND
  Increase the discriminatory power of PROSITE profiles to facilitate function determination and provide biologically relevant information about domains detected by profiles for the annotation of proteins.
  
  
  CONCLUSIONS
  We have created a new database, ProRule, which contains additional information about PROSITE profiles. ProRule contains notably the position of structurally and/or functionally critical amino acids, as well as the condition they must fulfill to play their biological role. These supplementary data should help function determination and annotation of the UniProt Swiss-Prot knowledgebase. ProRule also contains information about the domain detected by the profile in the Swiss-Prot line format. Hence, ProRule can be used to make Swiss-Prot annotation more homogeneous and consistent. The format of ProRule can be extended to provide information about combination of domains.
  
  
  BACKGROUND
  ProRule can be accessed through ScanProsite at http://www.expasy.org/tools/scanprosite. A file containing the rules will be made available under the PROSITE copyright conditions on our ftp site (ftp://www.expasy.org/databases/prosite/) by the next PROSITE release.
authors.affiliation: Swiss Institute of Bioinformatics (SIB), 1 rue Michel Servet, CH-1211 Geneva 4, Switzerland. christian.sigrist@isb-sib.ch
authors.forename: Christian J A
authors.initials: CJ
authors.lastname: Sigrist
authors.forename: Edouard
authors.initials: E
authors.lastname: De Castro
authors.forename: Petra S
authors.initials: PS
authors.lastname: Langendijk-Genevaux
authors.forename: Virginie
authors.initials: V
authors.lastname: Le Saux
authors.forename: Amos
authors.initials: A
authors.lastname: Bairoch
authors.forename: Nicolas
authors.initials: N
authors.lastname: Hulo
mesh_terms: D000595:Amino Acid Sequence; D003628:Database Management Systems; D030562:Databases, Protein; D016247:Information Storage and Retrieval / Q000379:methods*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D020539:Sequence Analysis, Protein / Q000379:methods*; D013329:Structure-Activity Relationship
chemical_list: D011506:Proteins