[Nine polymorphisms of fibrinogen gene and their association with plasma fibrinogen levels in Hainan Han population].
OBJECTIVE
To investigate the allelic frequencies of polymorphisms of alpha Taq I and beta Bcl I, Hinf I A/C, 448 G/A, beta BsmA I G/C, +1689T/G, -148C/T, -249C/T, -455G/A in Hainan Han population and their association with plasma fibrinogen level.


METHODS
Turbidmetric assay was used to measure plasma fibrinogen level of two hundred and thirty-eight healthy individuals. The genotypes were characterized by PCR-RFLP and sequence analysis. The relationships between the genotypes and plasma fibrinogen levels were analyzed by t test and ANOVA.


RESULTS
The frequencies of the rare alleles of alpha Taq I and beta Bcl I, Hinf I A/C, 448 G/A, beta BsmA I G/C, +1689T/G, -148C/T, -249C/T, -455G/A polymorphisms were 0.445, 0.239, 0.134, 0.235, 0.273, 0.241, 0.265, 0.441, 0.254 respectively. In the general population, the plasma fibrinogen level is significantly higher in the groups of genotypes -455GA and AA, -148CT and TT, alpha Taq I T1T1 than in the group of wild types(P=0.004, 0.015 and 0.043 respectively). In the men, plasma fibrinogen level is significantly higher in the groups of genotypes -455GA and AA, -148CT and TT, alpha Taq I T1T1, alpha Taq I T1T2 than in the group of wild types(P=0.001, 0.023, 0.003 and 0.032 respectively). In the women, no significant genotype association with plasma fibrinogen level was detected.


CONCLUSIONS
There was linkage disequilibrium between the fibrinogen gene loci. The beta -455G/A beta 448G/A, alpha Fg Taq I polymorphisms were associated with the difference in plasma fibrinogen in men. A(-455), T(-148) and alpha Taq I T1 alleles were associated with higher fibrinogen levels.
Liang
Liang
L
Department of Biochemistry, Hainan Medical College, Haikou, Hainan, 571101 PR China.
Sun
Chuan
C
Xiao
Feng
F
Tang
Xiao-lan
XL
Chen
Xiao-dan
XD
Zhou
Dai-feng
DF
Wang
Xiao-ying
XY
Wang
Zheng
Z
Liu
Guo-xun
GX
Cheng
Shuang
S
Ling
Guang-xin
GX
Bao
Cheng-xin
CX
Cai
Wang-wei
WW
D000328:Adult; D002681:China; D005260:Female; D005340:Fibrinogen / Q000235:genetics* / Q000378:metabolism; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D005340:Fibrinogen