title: Biochemical characterization and membrane fluidity of membranous vesicles isolated from boar seminal plasma.
abstract: Mammalian seminal plasma contains membranous vesicles (MV), which differ in composition and origin. Among these particles, human prostasomes and equine prostasome-like MV have been the most studied. The aim of the present work is to characterize the biochemical composition and membrane fluidity of MV isolated from boar seminal plasma. The MV from boar seminal plasma were isolated by ultracentrifugation and further purification by gel filtration on Sephadex G-200. The MV were examined by electron microscopy (EM), amount of cholesterol, total phospholipid, protein content, and phospholipid composition were analyzed. Membrane fluidity of MV and spermatozoa were estimated from the electron spin resonance (ESR) spectra of the 5-doxilstearic acid incorporated into the vesicle membranes by the order parameter (S). The S parameter gives a measure of degree of structural order in the membrane and is defined as the ratio of the spectral anisotropy in the membranes to the maximum anisotropy obtained in a rigidly oriented system. The S parameter takes into consideration that S = 1 for a rapid spin-label motion of about only one axis and S = 0 for a rapid isotropic motion. Intermediate S values between S = 0 and S = 1 represents the consequence of decreased membrane fluidity. The EM revealed the presence of bilaminar and multilaminar electron-dense vesicles. Cholesterol to phospholipid molar ratio from the isolated MV was 1.8. Phospholipid composition showed a predominance of sphingomyelin. The S parameter for porcine MV and for boar spermatozoa was 0.73 +/- 0.02 and 0.644 +/- 0.008, respectively, with the S for MV being greater (p < 0.001) than the S for spermatozoa. The high order for S found for boar MV was in agreement with the greater cholesterol/phospholipids ratio and the lesser ratio for phosphatidylcholine/sphingomyelin. Results obtained in the present work indicate that MV isolated from boar semen share many biochemical and morphological characteristics with equine prostasome-like MV and human prostasomes. The characteristics of the porcine MV of the seminal plasma, however, differed from those of boar sperm plasma membranes.
authors.affiliation: Cátedra de Física, Facultad de Farmacia y Bioquímica and Laboratorio Nacional de Investigación y Servicios en Radicales Libres en Biología y Medicina, Universidad de Buenos Aires, Junín 956, Planta Baja, 1113-Buenos Aires, Argentina. lpiehl@ffyb.uba.ar
authors.forename: Lidia L
authors.initials: LL
authors.lastname: Piehl
authors.forename: Humberto
authors.initials: H
authors.lastname: Cisale
authors.forename: Natalia
authors.initials: N
authors.lastname: Torres
authors.forename: Francisco
authors.initials: F
authors.lastname: Capani
authors.forename: Norma
authors.initials: N
authors.lastname: Sterin-Speziale
authors.forename: Alfredo
authors.initials: A
authors.lastname: Hager
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry* / Q000648:ultrastructure; D002784:Cholesterol / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy / Q000662:veterinary; D007425:Intracellular Membranes / Q000737:chemistry; D008297:Male; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000032:analysis / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D012661:Semen / Q000737:chemistry / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology*; D013552:Swine
chemical_list: D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol