HIV-1 genetic diversity in antenatal cohort, Canada.
We studied HIV genetic diversity in a cohort of 127 pregnant, HIV-infected women who received prenatal care at Sainte-Justine Hospital in Montreal, Canada, between 1999 and 2003. Clade assignments were derived by phylogenetic analysis of amplified pol sequences. Genotyping was successful in 103 of 127 women, 59 (57.3%) of whom were infected with clade B HIV-1, and 44 (42.7%) with nonclade B viruses, including subtypes A, C, D, F, G, and H. Four sequences remained unassigned. Forty-three of 44 women infected with non-clade B viruses were newcomers from sub-Saharan Africa, and subtype identity was consistent with those circulating in their countries of origin. These results highlight the epidemiologic importance of non-B HIV-1 in antenatal populations in a large North American urban center, underscore the influence of population movements on clade intermixing, and identify a group of patients who could be targeted for surveillance and drug therapy followup.
Akouamba
Bertine S
BS
Hôpital Sainte-Justine, Montreal, Quebec, Canada.
Viel
Janique
J
Charest
Hugues
H
Merindol
Natacha
N
Samson
Johanne
J
Lapointe
Normand
N
Brenner
Bluma G
BG
Lalonde
Richard
R
Harrigan
P Richard
PR
Boucher
Marc
M
Soudeyns
Hugo
H
D000328:Adult; D017954:Africa South of the Sahara / Q000208:ethnology; D001208:Asia / Q000208:ethnology; D002170:Canada / Q000453:epidemiology; D017691:Caribbean Region / Q000208:ethnology; D016000:Cluster Analysis; D015331:Cohort Studies; D005260:Female; D015684:Gene Products, pol / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D015658:HIV Infections / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D010802:Phylogeny; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000453:epidemiology / Q000821:virology*; D012367:RNA, Viral / Q000097:blood / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014505:Urban Population; D019562:Viral Load; D054302:pol Gene Products, Human Immunodeficiency Virus
D015684:Gene Products, pol; C060467:HIV pol protein p65; D012367:RNA, Viral; D054302:pol Gene Products, Human Immunodeficiency Virus