Microarray analysis of the genes induced by tetracycline-regulated expression of NDRF/NeuroD2 in P19 cells.
NeuroD-related factor (NDRF)/NeuroD2 is a basic helix-loop-helix (bHLH) protein that plays important roles in neuronal development. To elucidate the NDRF transcription network, we used mouse cDNA microarray analysis combined with a tetracycline-regulatable expression system in P19 embryonal carcinoma cells. Five genes were identified to be up-regulated in the presence of NDRF protein. RNA hybridization analysis confirmed that brain-lipid-binding protein (BLBP) and inhibitor of differentiation 1 (Id1) genes were among the five genes that were rapidly and significantly up-regulated after induction of NDRF. When a dominant negative form of NDRF protein was expressed during retinoic acid-induced neuronal differentiation of P19 cells, the BLBP gene, but not the Id1 gene, was potently repressed. Immunohistochemical analysis revealed that both NDRF and Id1 immunoreactivities were observed in some granule cells of the cerebellum in the postnatal period. These results suggest that NDRF or its related bHLH proteins may act upstream of these genes in a subset of developing neurons.
Oda
Hisanobu
H
Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 862-0976, Japan. histonecode@yahoo.co.jp
Fushimi
Fumiyoshi
F
Kato
Masaki
M
Kitagawa
Michinori
M
Araki
Kimi
K
Seki
Naohiko
N
Ohkubo
Hiroaki
H
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000196:embryology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000378:metabolism; D004318:Doxycycline / Q000494:pharmacology; D005786:Gene Expression Regulation; D005799:Genes, Dominant; D007150:Immunohistochemistry; D051798:Inhibitor of Differentiation Protein 1; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009479:Neuropeptides / Q000096:biosynthesis* / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D013752:Tetracycline / Q000494:pharmacology*; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology; D015854:Up-Regulation
D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary; D004318:Doxycycline; D051798:Inhibitor of Differentiation Protein 1; D009479:Neuropeptides; D012313:RNA; D012333:RNA, Messenger; D012097:Repressor Proteins; D013752:Tetracycline; D014157:Transcription Factors; D014212:Tretinoin; C497455:Idb1 protein, mouse; C098995:Neurod2 protein, mouse