Peptide-assisted synthesis of gold nanoparticles and their self-assembly.
A novel gold nanoparticle-tripeptide (GNP-tripeptide) conjugate was prepared by peptide in-situ redox technique at ambient temperatureusing a newly designed tripeptide. This new tripeptide was nso designed that it has a C-terminus tyrosine residue, which reduced Au+3 to Au, and the terminally located free amino group was bound to the gold nanoparticle (GNP) surface resulting in highly stable Au colloids. The average diameter of the tripeptide-stabilized GNP is 8.7 +/- 2.3 nm. Tripeptide bound gold nanoparticles formed three-dimensional assemblies in the presence of an excess of similar or disimilar tripeptides. The aggregation of GNPs results in a red shift in the surface plasmon resonance from lambda max = 527 to 556 nm. The effect of the solvent, concentration, and nature of the tripeptides on the assembly process were investigated by TEM and UV-visible spectroscopy.
Bhattacharjee
Rama Ranjan
RR
Polymer Science Unit Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700 032, India.
Das
Apurba K
AK
Haldar
Debasish
D
Si
Satyabrata
S
Banerjee
Arindam
A
Mandal
Tarun K
TK
D003102:Colloids / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D008956:Models, Chemical; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D043942:Nanotubes / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014443:Tyrosine / Q000737:chemistry; D014466:Ultraviolet Rays
D003102:Colloids; D046911:Macromolecular Substances; D010455:Peptides; D014443:Tyrosine; D006046:Gold