Automated genotyping of Saccharomyces cerevisiae using the RiboPrinter.
This research note addresses the development of an automated molecular typing system for yeast. Specifically, our objectives were to generate specific probes for genotyping yeast with an automated fingerprinting system. We have adapted the RiboPrinter microbial characterization system for use with Saccharomyces cerevisiae yeast using alternative probes based on specific multi-copy gene families. Manual construction and labeling of probes proved successful in initial experiments. Results indicate that this method could be applied to food or clinical environments if the appropriate probes are developed.
Arvik
Torey
T
Cornell University/New York State Agricultural Experiment Station, Department of Food Science and Technology, Food Science Laboratory Building, Geneva, New York 14456, USA. tja5@cornell.edu
Henick-Kling
Thomas
T
Gafner
Jürg
J
D004271:DNA, Fungal / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005838:Genotype; D021521:Ribotyping / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification*; D013045:Species Specificity
D004271:DNA, Fungal