Interleukin-10 promoter gene polymorphisms have no clear influence on interleukin-10 protein secretion in AIDS-associated B-cell lines.
Interleukin-10 (IL-10) is a pleiotropic cytokine involved in several immune responses and expressed by a variety of cell types. IL-10 interacts with at least two subunits of the IL-10 receptors (IL-10R1 and IL-10R2), which are members of the interferon receptor family, and play important roles in ligand binding and signaling. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) methods, the mRNA expression and secretion patterns of IL-10 were studied. IL-10R1 and IL-10R2 mRNA expression patterns were also studied in the tumor-derived human B-cell lines. IL-10 protein is expressed and predominantly secreted by AIDS-associated B-cell lines (AABCL). However, IL-10R1 and IL-10R2 are constitutively and ubiquitously expressed in all the B-cell lines included in our study. These results suggest that B-cell IL-10 functions as an autocrine growth factor, in AABCL. Furthermore, we report that higher secretion of IL-10 observed in AABCL could be due to the specific GCC haplotype of IL-10 promoter polymorphisms, although no specific correlation was observed between IL-10 promoter polymorphisms and IL-10 protein secretion as analyzed by PCR-sequence specific primers methodology and ELISA, respectively.
Huang
Yi-Chun
YC
Laboratory of Cytokine Research, Department of Biology, University of West Florida, Pensacola, FL 32514, USA.
Tsukamoto
Kana
K
Sharma
Venkatanarayanan
V
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood*; D000483:Alleles; D001402:B-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007107:Immune System; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013755:Tetradecanoylphorbol Acetate
D008024:Ligands; D012333:RNA, Messenger; D016753:Interleukin-10; D013755:Tetradecanoylphorbol Acetate