Application of polymerase chain reaction to detect adulteration of sheep's milk with goats' milk.
The polymerase chain reaction has been applied for the specific detection of goats' milk in sheep's milk using primers targeting the mitochondrial 12S ribosomal RNA gene. The use of goat-specific primers yielded a 122-bp fragment from goats' milk DNA, whereas no amplification signal was obtained in sheep's, cows', and water buffaloes' milk DNA. Polymerase chain reaction analysis of raw and heat-treated milk binary mixtures of sheep/goat enabled the specific detection of goats' milk with a sensitivity threshold of 0.1%. This study demonstrates the usefulness of the proposed polymerase chain reaction assay for authentication of milk products in routine analysis.
López-Calleja
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I
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
González
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I
Fajardo
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V
Martín
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I
Hernández
P E
PE
García
T
T
Martín
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R
D000818:Animals; D002020:Buffaloes; D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling; D006041:Goats / Q000235:genetics*; D006358:Hot Temperature; D008892:Milk / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000235:genetics; D012756:Sheep
D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D012313:RNA; D004247:DNA