Comparison of Microcapillary Cytometry Technology and Flow Cytometry for CD4+ and CD8+ T-Cell Estimation.
An alternative technology for the estimation of T cells based on a microcapillary technique (Guava Technologies, Hayward, CA) was compared to FACSCount (Becton Dickinson, San Jose, CA). Samples from 51 human immunodeficiency virus-infected and 21 healthy individuals were tested. The correlation (r) of the two systems for CD4(+) T cells was 0.994, and the coefficient of variation was 6.5%, establishing equable performance between the two technologies.
Kandathil
A J
AJ
Department of Clinical Virology, Christian Medical College, Vellore, 632004 Tamilnadu, India.
Kannangai
R
R
David
S
S
Nithyanandam
G
G
Solomon
S
S
Balakrishnan
P
P
Abraham
O C
OC
Subramanian
S
S
Rupali
P
P
Verghese
V P
VP
Pulimood
S
S
Sridharan
G
G
D016516:CD4-CD8 Ratio; D003592:Cytophotometry / Q000295:instrumentation* / Q000379:methods; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D006801:Humans; D018655:Lymphocyte Count / Q000295:instrumentation* / Q000379:methods; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*