In vivo reporter labeling of proteins via metabolic delivery of coenzyme A analogues.
We demonstrate site-specific reporter labeling of proteins within live cells. By accessing the coenzyme A (CoA) metabolic pathway with a cell-permeable pantetheine analogue, we deliver CoA-bound reporter molecules for post-translational protein modification in vivo. These methods may be applied to natural product pathway manipulation as well as applications in conventional molecular and cellular biology.
Clarke
Kristine M
KM
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0358, USA.
Mercer
Andrew C
AC
La Clair
James J
JJ
Burkart
Michael D
MD
D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D003065:Coenzyme A / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D010204:Pantetheine / Q000737:chemistry / Q000378:metabolism*
D002352:Carrier Proteins; D005456:Fluorescent Dyes; D010204:Pantetheine; D003065:Coenzyme A