Investigating the putative glycine hinge in Shaker potassium channel.
The crystal structure of an open potassium channel reveals a kink in the inner helix that lines the pore (Jiang, Y.X., A. Lee, J.Y. Chen, M. Cadene, B.T. Chait, and R. MacKinnon. 2002. Nature 417:523-526). The putative hinge point is a highly conserved glycine residue. We examined the role of the homologous residue (Gly466) in the S6 transmembrane segment of Shaker potassium channels. The nonfunctional alanine mutant G466A will assemble, albeit poorly, with wild-type (WT) subunits, suppressing functional expression. To test if this glycine residue is critical for activation gating, we did a glycine scan along the S6 segment in the background of G466A. Although all of these double mutants lack the higher-level glycosylation that is characteristic of mature Shaker channels, one (G466A/V467G) is able to generate voltage-dependent potassium current. Surface biotinylation shows that functional and nonfunctional constructs containing G466A express at comparable levels in the plasma membrane. Compared with WT channels, the shifted-glycine mutant has impairments in voltage-dependent channel opening, including a right-shifted activation curve and a decreased rate of activation. The double mutant has relatively normal open-channel properties, except for a decreased affinity for intracellular blockers, a consequence of the loss of the side chain of Val467. Control experiments with the double mutants M440A/G466A and G466A/V467A suggest that the flexibility provided by Gly466 is more important for channel function than its small size. Our results support roles for Gly466 both in biogenesis of the channel and as a hinge in activation gating.
Ding
Shinghua
S
Department of Physiology, Institute of Hyperexcitability, Jefferson Medical College, Philadelphia, PA 19107, USA.
Ingleby
Lindsey
L
Ahern
Christopher A
CA
Horn
Richard
R
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D000939:Epitopes / Q000032:analysis; D005998:Glycine / Q000737:chemistry* / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D015640:Ion Channel Gating / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D009154:Mutation; D009842:Oligopeptides; D009865:Oocytes / Q000378:metabolism; D010455:Peptides / Q000032:analysis; D017354:Point Mutation; D011188:Potassium / Q000378:metabolism; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015221:Potassium Channels / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017433:Protein Structure, Secondary; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D051637:Shaker Superfamily of Potassium Channels; D014162:Transfection; D014982:Xenopus laevis
D000939:Epitopes; D009842:Oligopeptides; D010455:Peptides; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D000644:Quaternary Ammonium Compounds; D051637:Shaker Superfamily of Potassium Channels; C085706:FLAG peptide; C027435:tetrapentylammonium; D011188:Potassium; D005998:Glycine