title: Enzymatic detergent formulation containing amylase from Aspergillus niger: a comparative study with commercial detergent formulations.
abstract: There is a wide range of biotechnological applications for amylases, including the textile, pharmaceutical, food and laundry industries. Hydrolytic enzymes are 100% biodegradable and enzymatic detergents can achieve effective cleaning with lukewarm water. Microorganisms and culture media were tested for amylase production and the best producer was Aspergillus niger L119 (3.9 U ml(-1) +/- 0.2) in submerged culture and its amylase demonstrated excellent activity at 50-55 degrees C and pH 4.0, remaining stable at 53 degrees C for up to 200 h. In order to establish the potential uses of this enzyme in detergents, different formulations were tested using the A. niger amylase extract. Enzyme activity was compared with three commercial formulations. The detergents are used in hospitals to clean surgical and endoscopy equipment. The presence of amylase in the formulation is because of its action within hospital drainage system, whether or not it has any function in cleaning the equipment.
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, CEP 91501-970, Porto Alegre, RS, Brazil. sydnei@mitidieri.com.br
authors.forename: Sydnei
authors.initials: S
authors.lastname: Mitidieri
authors.forename: Anne Helene
authors.initials: AH
authors.lastname: Souza Martinelli
authors.forename: Augusto
authors.initials: A
authors.lastname: Schrank
authors.forename: Marilene Henning
authors.initials: MH
authors.lastname: Vainstein
mesh_terms: D000681:Amylases / Q000737:chemistry*; D000704:Analysis of Variance; D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics; D003470:Culture Media; D003902:Detergents / Q000737:chemistry*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D013997:Time Factors
chemical_list: D003470:Culture Media; D003902:Detergents; D000681:Amylases