title: Ultrastructural effects of antimicrobial peptides from bovine lactoferrin on the membranes of Candida albicans and Escherichia coli.
abstract: Antimicrobial peptides allegedly exert their action on microbial membranes. Bovine lactoferrin enfold two antimicrobial domains, lactoferricin B (LFcin B) and lactoferrampin (LFampin). Effects of representative peptides thereof on the membranes of Candida albicans and Escherichia coli were investigated. Confocal laser scanning microscopy revealed that these peptides were internalized within a few minutes, concurrently with disrupting membrane integrity as indicated by freeze-fracture transmission electron microscopy. The most striking findings were induction of distinct vesicle-like structures in the membrane of C. albicans by the LFampin peptide, and detachment of the outer membrane and surface protrusions in E. coli by the LFcin B peptide.
authors.affiliation: Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit and Universiteit van Amsterdam, Van der Boechorststraat 7, 1081BT Amsterdam, The Netherlands.
authors.forename: Marieke I A
authors.initials: MI
authors.lastname: van der Kraan
authors.forename: Jan
authors.initials: J
authors.lastname: van Marle
authors.forename: Kamran
authors.initials: K
authors.lastname: Nazmi
authors.forename: Jasper
authors.initials: J
authors.lastname: Groenink
authors.forename: Wim
authors.initials: W
authors.lastname: van 't Hof
authors.forename: Enno C I
authors.initials: EC
authors.lastname: Veerman
authors.forename: Jan G M
authors.initials: JG
authors.lastname: Bolscher
authors.forename: Arie V
authors.initials: AV
authors.lastname: Nieuw Amerongen
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects* / Q000648:ultrastructure; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005614:Freeze Fracturing; D007781:Lactoferrin / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry; D018613:Microscopy, Confocal; D008854:Microscopy, Electron / Q000379:methods; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology
chemical_list: D023181:Antimicrobial Cationic Peptides; D007782:Lactoglobulins; D010446:Peptide Fragments; C502040:lactoferrampin; C082171:lactoferricin B; D007781:Lactoferrin; D016650:Fluorescein-5-isothiocyanate