Spectral study of interactions of 4,8,4'-trimethylpsoralen and 4,4'-dimethylangelicin dyes with DNA.
Absorption and fluorescence spectra for six new synthetic dyes of 4,8,4'-trimethylpsoralen and 4,4'-dimethylangelicin derivatives containing various terminal substituents at 5'-position have been investigated in different environments using a wide range of the DNA/ligand concentrations. Various spectral and binding characteristics of the DNA-ligand systems have been determined. General principles characterizing mechanisms responsible for changes in the fluorescent properties of nucleotide-specific dyes have been proposed; they take into consideration chemical structure of the dyes, properties of the environment, and degree of sorption on substrate.
Sibirtsev
V S
VS
Mendeleyev Russian Research Institute for Metrology, St. Petersburg, 190005, Russia. vs1969r@mail.ru
Tolmachev
A Yu
AY
Kovaleva
M V
MV
Garabadzhiu
A V
AV
Traven
V F
VF
D004396:Coloring Agents / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D011564:Furocoumarins / Q000138:chemical synthesis / Q000737:chemistry*; D008024:Ligands; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods; D014307:Trioxsalen / Q000138:chemical synthesis / Q000737:chemistry*
D004396:Coloring Agents; D011564:Furocoumarins; D008024:Ligands; C038161:4,4'-dimethylangelicin; D004247:DNA; D014307:Trioxsalen