title: Complete genome sequence and lytic phase transcription profile of a Coccolithovirus.
abstract: The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi. Among the 472 predicted genes of the 407,339-base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi.
authors.affiliation: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK. whw@pml.ac.uk
authors.forename: William H
authors.initials: WH
authors.lastname: Wilson
authors.forename: Declan C
authors.initials: DC
authors.lastname: Schroeder
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Allen
authors.forename: Matthew T G
authors.initials: MT
authors.lastname: Holden
authors.forename: Julian
authors.initials: J
authors.lastname: Parkhill
authors.forename: Bart G
authors.initials: BG
authors.lastname: Barrell
authors.forename: Carol
authors.initials: C
authors.lastname: Churcher
authors.forename: Nancy
authors.initials: N
authors.lastname: Hamlin
authors.forename: Karen
authors.initials: K
authors.lastname: Mungall
authors.forename: Halina
authors.initials: H
authors.lastname: Norbertczak
authors.forename: Michael A
authors.initials: MA
authors.lastname: Quail
authors.forename: Claire
authors.initials: C
authors.lastname: Price
authors.forename: Ester
authors.initials: E
authors.lastname: Rabbinowitsch
authors.forename: Danielle
authors.initials: D
authors.lastname: Walker
authors.forename: Marie
authors.initials: M
authors.lastname: Craigon
authors.forename: Douglas
authors.initials: D
authors.lastname: Roy
authors.forename: Peter
authors.initials: P
authors.lastname: Ghazal
mesh_terms: D017209:Apoptosis; D001482:Base Composition; D002518:Ceramides / Q000096:biosynthesis; D019295:Computational Biology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D056890:Eukaryota / Q000821:virology; D015870:Gene Expression; D020869:Gene Expression Profiling; D005814:Genes, Viral; D016679:Genome, Viral; D020411:Oligonucleotide Array Sequence Analysis; D010447:Peptide Hydrolases / Q000235:genetics; D017862:Phycodnaviridae / Q000145:classification / Q000235:genetics* / Q000502:physiology*; D011401:Promoter Regions, Genetic; D021122:Protein Subunits; D017422:Sequence Analysis, DNA; D013107:Sphingolipids / Q000096:biosynthesis; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D002518:Ceramides; D004279:DNA, Viral; D021122:Protein Subunits; D013107:Sphingolipids; D012321:DNA-Directed RNA Polymerases; D010447:Peptide Hydrolases