title: West Nile virus detection in urine.
abstract: We report West Nile virus (WNV) RNA in urine collected from a patient with encephalitis 8 days after symptom onset. Viral RNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Sequence and phylogenetic analysis confirmed the PCR product to have > or = 99% similarity to the WNV strain NY 2000-crow3356.
authors.affiliation: Asia Pacific Institute of Tropical Medicine and Infectious Diseases John A. Burns School of Medicine, Honolulu, Hawaii 96816, USA. jtonry@rocketmail.com
authors.forename: Jessica H
authors.initials: JH
authors.lastname: Tonry
authors.forename: Craig B
authors.initials: CB
authors.lastname: Brown
authors.forename: Cecil B
authors.initials: CB
authors.lastname: Cropp
authors.forename: Juliene K G
authors.initials: JK
authors.lastname: Co
authors.forename: Shannon N
authors.initials: SN
authors.lastname: Bennett
authors.forename: Vivek R
authors.initials: VR
authors.lastname: Nerurkar
authors.forename: Timothy
authors.initials: T
authors.lastname: Kuberski
authors.forename: Duane J
authors.initials: DJ
authors.lastname: Gubler
mesh_terms: D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D016000:Cluster Analysis; D006801:Humans; D008297:Male; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014901:West Nile Fever / Q000188:drug therapy / Q000652:urine*; D014902:West Nile virus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral