title: [The determination of echinacoside and acteoside in herbs of Cistanche tubulosa].
abstract: OBJECTIVE
  To analyse the echinacoside and acteoside content of Cistanche tubulosa, collected from different hosts and different size of the cultivated, which is compared to the wilding by RP-HPLC method.
  
  
  METHODS
  An Agilent Eclipse XDB-C18(4.6 mm x 250 mm, 5 microm) column was used and a mixture of methanol-acetonitrile-1% acetic acid (15:10:75) was used as the mobile phase at a flow rate of 0.6 mL x min(-1). The column temperature was 30 degrees C and the UV detection wavelength was 334 nm.
  
  
  RESULTS
  The calibration curves of echinacoside and acteoside were in good linearity over the range of 0.904-9.04 microg (r = 0.999 9), and 1.27-12.7 microg (r = 0.999 9) respectively and the average recoveries of echinacoside (and acteoside) were 98.9% (n = 5, RSD 1.9%), and 97.0% (n = 5, RSD 0.97%).
  
  
  CONCLUSIONS
  The method is simple, quick, acurate. In all of the samples, the contents of echinacoside is markedly more than that of acteoside, the content of the two active component in the wilding is higher than that in the planting. The content of sample in the different sizes gradually increase from the big to the small, and the contents of samples collected from the different hosts vary markedly. These results are useful for the quality evaluation of medicinal materials of C. tubulosa.
authors.affiliation: Institute of Chinese Materia Medica, Chinese Academy of Traditional Chinese Medicine, Beijing 100700, China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Su-Ping
authors.initials: SP
authors.lastname: Xiao
authors.forename: Guang-Hong
authors.initials: GH
authors.lastname: Cui
authors.forename: Si-Ju
authors.initials: SJ
authors.lastname: Zhang
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wu
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
authors.forename: Ming-Ting
authors.initials: MT
authors.lastname: Liu
mesh_terms: D001947:Breeding; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031671:Cistanche / Q000033:anatomy & histology / Q000737:chemistry* / Q000502:physiology; D005960:Glucosides / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D006790:Host-Parasite Interactions; D010636:Phenols / Q000032:analysis*; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000737:chemistry* / Q000502:physiology; D032363:Tamaricaceae / Q000145:classification / Q000469:parasitology; D059808:Polyphenols
chemical_list: D005960:Glucosides; D006027:Glycosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols; C060297:echinacoside