Excitation energy trapping in photosystem I complexes depleted in Lhca1 and Lhca4.
We report a time-resolved fluorescence spectroscopy characterization of photosystem I (PSI) particles prepared from Arabidopsis lines with knock-out mutations against the peripheral antenna proteins of Lhca1 or Lhca4. The first mutant retains Lhca2 and Lhca3 while the second retains one other light-harvesting protein of photosystem I (Lhca) protein, probably Lhca5. The results indicate that Lhca2/3 and Lhca1/4 each provides about equally effective energy transfer routes to the PSI core complex, and that Lhca5 provides a less effective energy transfer route. We suggest that the specific location of each Lhca protein within the PSI-LHCI supercomplex is more important than the presence of so-called red chlorophylls in the Lhca proteins.
Ihalainen
Janne A
JA
Vrije Universiteit Amsterdam, Department of Physics and Astronomy, Biophysics, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands. janihal@nat.vu.nl
Klimmek
Frank
F
Ganeteg
Ulrika
U
van Stokkum
Ivo H M
IH
van Grondelle
Rienk
R
Jansson
Stefan
S
Dekker
Jan P
JP
D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D061146:Chlorophyll Binding Proteins; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D045331:Photosystem I Protein Complex / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D029681:Arabidopsis Proteins; D061146:Chlorophyll Binding Proteins; C490278:LHCA1 protein, Arabidopsis; C490277:Lhca2 protein, Arabidopsis; C493777:Lhca5 protein, Arabidopsis; D045342:Light-Harvesting Protein Complexes; D045331:Photosystem I Protein Complex; D010940:Plant Proteins