There is no association between K469E ICAM-1 gene polymorphism and biliary atresia.
OBJECTIVE
To determine whether there was an association between inter-cellular adhesion molecule-1 (ICAM-1) gene polymorphism and biliary atresia (BA), and to investigate the relationship between serum soluble ICAM-1 (sICAM-1) and clinical outcome in BA patients after surgical treatment.


METHODS
Eighty-three BA patients and 115 normal controls were genotyped. K469E ICAM-1 polymorphism was analyzed using PCR assay. Serum sICAM-1 was determined using ELISA method from 72 BA patients. In order to evaluate the association between these variables and their clinical outcome, the patients were categorized into two groups: patients without jaundice and those with persistent jaundice.


RESULTS
There were no significant differences between BA patients and controls in terms of gender, K469E ICAM-1 genotypes, and alleles. The proportion of patients having serum sICAM-1 >=3 500 ng/mL in persistent jaundice group was significantly higher than that in the other group. In addition, there was no association between K469E ICAM-1 polymorphism and the status of jaundice in BA patients after Kasai operation.


CONCLUSIONS
ICAM-1 possibly plays an important and active role in the disease progression. However, the process is not associated with genetic variation of K469E ICAM-1 polymorphism.
Vejchapipat
Paisarn
P
Pediatric Surgery Unit, Department of Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Jirapanakorn
Naruemol
N
Thawornsuk
Nutchanart
N
Theamboonlers
Apiradee
A
Chongsrisawat
Voranush
V
Chittmittrapap
Soottiporn
S
Poovorawan
Yong
Y
D000293:Adolescent; D000328:Adult; D019943:Amino Acid Substitution; D001483:Base Sequence; D001656:Biliary Atresia / Q000097:blood / Q000235:genetics* / Q000601:surgery; D002648:Child; D004247:DNA / Q000235:genetics; D017931:DNA Primers; D005260:Female; D005838:Genotype; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood / Q000235:genetics*; D008297:Male; D020125:Mutation, Missense; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide
D017931:DNA Primers; D018799:Intercellular Adhesion Molecule-1; D004247:DNA