Fate redirection of hippocampal astrocytes toward neuronal lineage by aggregate culture.
Mammalian cells that have been committed to a certain cell lineage cannot be directed to other lineages. However, some astrocytes in the mammalian brains have been reported to represent plasticity to redirect to other cell lineages. We found that mouse hippocampal astrocytes cultured in aggregate forms of "astrosphere", redirected to MAP2-positive immature neurons. In astrospheres, basic HLH factors positively regulating neuronal differentiation were up-regulated and Id3 inhibiting basic HLH factors was down-regulated. Ectopic Id3 induction repressed redirection of astrocytes to a neuronal lineage, suggesting that astrosphere formation induced plasticity of astrocytes by changing the gene expression patterns.
Yanagisawa
Makoto
M
Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan. myanagisawa@mail.mcg.edu
Nakashima
Kinichi
K
Ochiai
Wataru
W
Takizawa
Takumi
T
Setoguchi
Takao
T
Uemura
Atsumi
A
Takizawa
Makiko
M
Nobuhisa
Ikuo
I
Taga
Tetsuya
T
D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D002449:Cell Aggregation; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology*; D007150:Immunohistochemistry; D051796:Inhibitor of Differentiation Proteins; D051379:Mice; D020932:Nerve Growth Factor / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D011506:Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051796:Inhibitor of Differentiation Proteins; D011506:Proteins; C067679:Idb3 protein, mouse; D020932:Nerve Growth Factor