Modification of the intrinsic fluorescence and the biochemical behavior of ATP after irradiation with visible and near-infrared laser light.
In this work, the effects of visible (655 nm) and near-infrared (830 nm) light on ATP in solution were examined. The addition of irradiated ATP to the hexokinase reaction caused significant differences in the reaction rates and in the Michaelis-Menten kinetic parameters, k(m) and v(max). Irradiated ATP cleavage by hexokinase occurred in less time. Changes were wavelength and dose dependent. Excitation of ATP with a 260 nm wavelength ultraviolet light induced a fluorescence emission that was decreased when Mg2+ was added due to ion binding of the phosphates, which are the structures that modify the fluorescence produced by the adenine dipoles. The irradiation of this ATP.Mg2+ solution using 655 and 830 nm light increased the fluorescence by a possible displacement of Mg2+ from the phosphates. In conclusion, visible and near-infrared light modifies the biochemical behavior of ATP in the hexokinase reaction and the fluorescence intensity of the molecule thus altering the Mg2+ binding strength to the oxygen atoms in the phosphate group.
Amat
Albert
A
Histology and Neurobiology Unit, Faculty of Medicine and Health Sciences, Rovira i Virgili University, c. Sant Llorenç 21, 43201 Reus, Spain. albert.amat@urv.net
Rigau
Josepa
J
Waynant
Ronald W
RW
Ilev
Ilko K
IK
Tomas
Josep
J
Anders
Juanita J
JJ
D000244:Adenosine Diphosphate / Q000528:radiation effects; D000249:Adenosine Monophosphate / Q000528:radiation effects; D000255:Adenosine Triphosphate / Q000378:metabolism / Q000528:radiation effects*; D005453:Fluorescence; D006593:Hexokinase / Q000378:metabolism*; D007259:Infrared Rays; D007700:Kinetics; D028022:Low-Level Light Therapy; D008274:Magnesium
D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006593:Hexokinase; D008274:Magnesium