Solar-simulated ultraviolet radiation induces abnormal maturation and defective chemotaxis of dendritic cells.
Exposure to ultraviolet (UV) light induces immunosuppression. Different evidences indicate that this phenomenon is mainly a consequence of the effect of UV light on skin dendritic cells (DC). To investigate the cellular and molecular basis of this type of immunosuppression, we assessed in vitro the effect of solar-simulated UV radiation on the phenotypic and functional characteristics of human monocyte-derived DC and Langerhans-like DC. UV radiation induced a decreased expression of molecules involved in antigen capture as DC-SIGN and the mannose receptor. This effect was accompanied by a diminished endocytic capacity, an enhanced expression of molecules involved in antigen presentation such as major histocompatibility complex-II and CD86, and a significant increase in their capability to stimulate T cells. Furthermore, irradiated DC failed to acquire a full mature phenotype upon treatment with lipopolysaccharide. On the other hand, solar-simulated radiation induced the secretion of tumor necrosis factor-alpha and interleukin (IL)-10 by DC, but no IL-12. Interestingly, solar-simulated UV radiation also caused an altered migratory phenotype, with an increased expression of CXCR4, and a lack of induction of CCR7, thus correlating with a high chemotactic response to stromal cell-derived factor 1(SDF-1) (CXCL12), but not to secondary lymphoid tissue chemokine (SLC) (CCL21). These data indicate that solar-simulated UV radiation induces a defective maturation and an anomalous migratory phenotype of DC.
Mittelbrunn
María
M
Servicio de Inmunología, Hospital de la Princesa Universidad Autónoma de Madrid, Madrid, Spain.
Tejedor
Reyes
R
de la Fuente
Hortensia
H
García-López
M Angeles
MA
Ursa
Angeles
A
Peñas
Pablo F
PF
García-Díez
Amaro
A
Alonso-Lebrero
José Luis
JL
Pivel
Juan Pablo
JP
González
Salvador
S
Gonzalez-Amaro
Roberto
R
Sánchez-Madrid
Francisco
F
D017209:Apoptosis / Q000276:immunology / Q000528:radiation effects; D002450:Cell Communication / Q000276:immunology / Q000528:radiation effects; D002454:Cell Differentiation / Q000276:immunology / Q000528:radiation effects; D002634:Chemotaxis, Leukocyte / Q000276:immunology / Q000528:radiation effects*; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D009000:Monocytes / Q000166:cytology; D013472:Sunlight / Q000009:adverse effects; D013601:T-Lymphocytes / Q000166:cytology; D014466:Ultraviolet Rays / Q000009:adverse effects*
D016207:Cytokines