DNA-dependent conversion of Oct-1 and Oct-2 into transcriptional repressors by Groucho/TLE.
POU domain proteins contain a bipartite DNA-binding element that can confer allosteric control of coactivator recruitment. Dimerization of Oct-1 and Oct-2 on palindromic response elements results in the conformational dependent inclusion or exclusion of the transcriptional coactivator OBF-1. In this paper, we demonstrate that Oct-1 and Oct-2 can function as transcriptional repressors by recruiting and physically interacting with members of the Grg/TLE family of corepressors. In accordance with a model of DNA induced cofactor assembly, and analogous to the recruitment of the OBF-1 coactivator, the different Grg/TLE members can discriminate between both Oct-1 and Oct-2, and the monomeric or dimeric nature of the POU/DNA complex.
Malin
Stephen
S
Department of Microbiology and Tumor biology Box 280, Karolinska Institutet, 171 77 Stockholm, Sweden.
Linderson
Ylva
Y
Almqvist
Jenny
J
Ernberg
Ingemar
I
Tallone
Tiziano
T
Pettersson
Sven
S
D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D001665:Binding Sites; D005075:Biological Evolution; D002460:Cell Line; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D019281:Dimerization; D006801:Humans; D050811:Octamer Transcription Factor-1; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*; D020218:Response Elements; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D015533:Transcriptional Activation
D051792:Basic Helix-Loop-Helix Proteins; D004247:DNA; D004268:DNA-Binding Proteins; D050811:Octamer Transcription Factor-1; D012097:Repressor Proteins; D014157:Transcription Factors; C494165:POU2F1 protein, human; C091023:gro protein, Drosophila