title: Effects of phase I periodontal treatment on gingival crevicular fluid levels of matrix metalloproteinase-3 and tissue inhibitor of metalloproteinase-1.
abstract: OBJECTIVE
  The aim of this study was to evaluate the effects of phase I periodontal treatment on gingival crevicular fluid (GCF) levels of matrix metalloproteinase (MMP)-3 and tissue inhibitors of metalloproteinase (TIMP)-1.
  
  
  METHODS
  Plaque index, gingival index, pocket depth and clinical attachment loss were recorded and GCF samples were collected from 20 chronic periodontitis (CP) patients and 20 periodontally healthy controls (C) before treatment. CP patients received phase I periodontal treatment and all clinical parameters were recorded and GCF samples were collected once more after treatment. Assays were performed by an enzyme-linked immunosorbent assay.
  
  
  RESULTS
  All of the clinical parameters improved significantly after the therapy (p<0.05). Baseline GCF levels of MMP-3 were significantly higher than C and that level was reduced significantly by treatment compared with baseline levels (p<0.05). Baseline GCF levels of TIMP-1 were lower than post-treatment levels and C (p<0.05). GCF levels of TIMP-1 increased significantly by treatment compared with baseline levels (p<0.05).
  
  
  CONCLUSIONS
  This study shows that the clinical improvements after phase I periodontal therapy are accompanied by reduction in MMP-3 and increasing in TIMP-1 GCF levels.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey. gulay@gazi.edu.tr
authors.forename: Gülay
authors.initials: G
authors.lastname: Tüter
authors.forename: Bülent
authors.initials: B
authors.lastname: Kurtiş
authors.forename: Muhittin
authors.initials: M
authors.lastname: Serdar
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Yücel
authors.forename: Eylem
authors.initials: E
authors.lastname: Ayhan
authors.forename: Burcu
authors.initials: B
authors.lastname: Karaduman
authors.forename: Gönen
authors.initials: G
authors.lastname: Ozcan
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002908:Chronic Disease; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000032:analysis*; D010518:Periodontitis / Q000201:enzymology* / Q000628:therapy; D018709:Statistics, Nonparametric; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis*
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D019278:Matrix Metalloproteinase 3