Application of PCR, rep-PCR and RAPD techniques for typing of Lactococcus lactis strains.
A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for the acmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic identification of Lactococcus lactis strains by RAPD; rep-PCR did not distinguish between L. lactis subspecies. In some cases, phenotypic classification of L. lactis subspecies did not correlate with genotypic characterization.
Prodelalová
J
J
Department of Microbiology, Faculty of Science, Masaryk University, 602 00 Brno, Czechia. prodelalova@vri.cz
Spanová
A
A
Rittich
B
B
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D013294:Lactococcus lactis / Q000145:classification* / Q000235:genetics; D009113:Muramidase / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C092497:AcmA protein, Lactococcus lactis; D009113:Muramidase