title: Expression of heparanase mRNA in anti-sense oligonucleotide-transfected human esophageal cancer EC9706 cells.
abstract: OBJECTIVE
  To investigate the effects of anti-sense oligonucleotides (ASODNs) on mRNA expression of heparanase in human esophageal cancer EC9706 cells.
  
  
  METHODS
  One non-sense oligonucleotide (N-ODN) and five ASODNs against different heparanase mRNA sites were transfected into EC9706 cells, then the expression of heparanase mRNA in EC9706 cells was studied by in situ hybridization.
  
  
  RESULTS
  The expression of heparanase mRNA could be inhibited by ASODNs. There was no significant difference among five ASODNs (P>0.05), but there was a significant difference between ASODNs and N-ODN or non-transfected group (ASODN1: 2.25+/-0.25, ASODN2: 2.21+/-0.23, ASODN3: 2.23+/-0.23, ASODN4: 2.25+/-0.24 vs N-ODN: 3.47+/-2.80 or non- transfected group: 3.51+/-2.93 respectively, P<0.05).
  
  
  CONCLUSIONS
  The expression of heparanase mRNA in EC9706 cells can be inhibited by ASODNs in vivo, and heparanase ASODNs can inhibit metastasis of esophageal squamous cell carcinoma or other tumors by inhibiting the expression of heparanase.
authors.affiliation: Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Key Laboratory of Henan Tumor Pathology, Zhengzhou 450052, Henan Province, China. chenksh2002@yahoo.com.cn
authors.forename: Kui-Sheng
authors.initials: KS
authors.lastname: Chen
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
authors.forename: Yun-Han
authors.initials: YH
authors.lastname: Zhang
authors.forename: Dong-Ling
authors.initials: DL
authors.lastname: Gao
authors.forename: Liang
authors.initials: L
authors.lastname: Yan
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005966:Glucuronidase / Q000235:genetics*; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D014162:Transfection; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D000099175:Heparanase