title: Molecular characterization of Egyptian Trichomonas vaginalis clinical isolates by HSP70 restriction fragment length polymorphism.
abstract: Molecular typing of 20 Egyptian Trichomonas vaginalis clinical isolates was performed using the Restriction Fragment Length Polymorphism (RFLP) analysis employing a probe from the heat-inducible cytoplasmic HSP70 gene family hybridized with EcoR l-digested genomic DNA. In each of the isolates tested 5 to 6 distinct DNA fragments ranging from 2.7 Kb to 7.5 Kb in size were detected. Analysis of 13 isolates from symptomatic and 7 isolates from asymptomatic women revealed 6 distinct RFLP pattern subtypes of T. vaginalis. Eleven isolates (55%) showed the same RFLP pattern, teen of them (90.9%) were from symptomatic patients. T. vaginalis virus (TVV) was present in 7 isolates (35%). Only one isolate was considered resistant to Metronidazole. There were no relations between TVV infection or Metronidazole susceptibility and RFLP subtypes.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
authors.forename: Eman M
authors.initials: EM
authors.lastname: Hussien
authors.forename: Hanan Z
authors.initials: HZ
authors.lastname: El-Sayed
authors.forename: Amal A
authors.initials: AA
authors.lastname: El-Moamly
authors.forename: Moshira M
authors.initials: MM
authors.lastname: Helmy
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Shaban
mesh_terms: D000818:Animals; D000994:Antitrichomonal Agents / Q000494:pharmacology; D016054:DNA, Protozoan / Q000737:chemistry*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology; D008970:Molecular Weight; D012150:Polymorphism, Restriction Fragment Length; D014247:Trichomonas Vaginitis / Q000469:parasitology*; D014246:Trichomonas vaginalis / Q000737:chemistry / Q000145:classification* / Q000235:genetics
chemical_list: D000994:Antitrichomonal Agents; D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D008795:Metronidazole