Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates.
A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of K. pneumoniae isolates.
Diancourt
Laure
L
Unité Biodiversité des Bactéries Pathogènes Emergentes (U389 INSERM), Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris, Cedex 15, France.
Passet
Virginie
V
Verhoef
Jan
J
Grimont
Patrick A D
PA
Brisse
Sylvain
S
D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D003428:Cross Infection / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D021521:Ribotyping; D017422:Sequence Analysis, DNA