Phenolsulfonphthalein transport by potential-sensitive urate transport system.
The purpose of this study was to elucidate the transporter-mediated secretion systems for phenolsulfonphthalein in brush-border membranes. In human and rat renal brush-border membranes, a potential-sensitive transport system has been shown to be involved in the efflux of organic anions. The uptake of phenolsulfonphthalein into rat renal brush-border membrane vesicles was stimulated by an inside-positive membrane potential. This potential-sensitive uptake of phenolsulfonphthalein was inhibited by probenecid, pyrazinoate and urate. p-Aminohippurate had no effect on the potential-sensitive uptake of phenolsulfonphthalein. Moreover, urate competitively inhibited the uptake of phenolsulfonphthalein. On the other hand, the uptake of phenolsulfonphthalein was slightly increased in the presence of an outward Cl- gradient. These results suggest that phenolsulfonphthalein has high affinity for the potential-sensitive urate transport system but has low affinity for an anion exchanger.
Itagaki
Shirou
S
Department of Clinical Pharmaceutics and Therapeutics, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12-jo, Nishi 6-chome, Kita-ku, Sapporo 060-0812, Japan.
Shimamoto
Soji
S
Sugawara
Mitsuru
M
Kobayashi
Michiya
M
Miyazaki
Katsumi
K
Hirano
Takeshi
T
Iseki
Ken
K
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D002712:Chlorides / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007476:Ionophores / Q000494:pharmacology; D007668:Kidney / Q000166:cytology / Q000648:ultrastructure; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008564:Membrane Potentials / Q000502:physiology; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism; D010637:Phenolsulfonphthalein / Q000493:pharmacokinetics* / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D011718:Pyrazinamide / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014527:Uric Acid / Q000493:pharmacokinetics* / Q000494:pharmacology; D014634:Valinomycin / Q000494:pharmacology
D002712:Chlorides; D007476:Ionophores; D014634:Valinomycin; D014527:Uric Acid; D011718:Pyrazinamide; C005296:pyrazinoic acid; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D008353:Mannitol; D011189:Potassium Chloride; D010637:Phenolsulfonphthalein; D011339:Probenecid