Immunophenotypic changes induced on human HL60 leukaemia cells by 1alpha,25-dihydroxyvitamin D3 and 12-O-tetradecanoyl phorbol-13-acetate.
1alpha,25-Dihydroxyvitamin D3 (1,25D3) induces HL60 cells to acquire a monocyte-like phenotype, while cells treated with 12-O-tetradecanoyl phorbol-13-acetate (TPA) resemble macrophages. Using a microarray of 82 CD antibodies, 24 cluster of differentiation (CD) antigens were detected on HL60 cells. 1,25D3 induced the following antigens in decreasing order of the change: CD14, CD11c, CD11b, CD54, CD86, CD38 and CD66c, with repression of CD117, CD71, CD95, CD45 and CD64. TPA induced the following antigens in decreasing order of the change: CD11c, CD9, CD11b, CD54, CD38, CD45RO and CD66c, with repression of CD4, CD117, CD95, CD71 and CD64. The results presented provide a basis for monitoring differentiation therapy of myeloid leukaemias in patients.
White
Sarah L
SL
School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia.
Belov
Larissa
L
Barber
Nicole
N
Hodgkin
Philip D
PD
Christopherson
Richard I
RI
D015703:Antigens, CD / Q000378:metabolism; D002117:Calcitriol / Q000494:pharmacology*; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D002273:Carcinogens / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020869:Gene Expression Profiling; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D046228:Microarray Analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D015703:Antigens, CD; D002120:Calcium Channel Agonists; D002273:Carcinogens; D002117:Calcitriol; D013755:Tetradecanoylphorbol Acetate