Two novel lectins from Parkia biglandulosa and Parkia roxburghii: isolation, physicochemical characterization, mitogenicity and anti-proliferative activity.
Two mannose/glucose specific seed lectins were isolated from Parkia biglandulosa and Parkia roxburghii and were characterized w.r.t various physicochemical properties. Unlike other Parkia lectins a comparison of native and subunit molecular mass showed that both Parkia lectins were heterotetramers. Parkia biglandulosa lectin was found to be T-cell mitogen as revealed by IL-2 bioassay. These lectins showed anti-proliferative effect on two murine macrophage cancer cell lines i.e. P 388DI (50%) and J774 (70%). In addition Parkia roxburghii also inhibited proliferation of HB98 (65.47%), a B-cell hybridoma cell line.
Kaur
Navjot
N
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar-143005, India.
Singh
Jatinder
J
Kamboj
Sukhdev Singh
SS
Agrewala
Javed N
JN
Kaur
Manpreet
M
D000818:Animals; D050260:Carbohydrate Metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008934:Mitogens / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D013379:Substrate Specificity
D008934:Mitogens; D037121:Plant Lectins