Requirements of myocyte-specific enhancer factor 2A in zebrafish cardiac contractility.
Myocyte-specific enhancer factor 2A (MEF2A) regulates a broad range of fundamental cellular processes including cell division, differentiation and death. Here, we tested the hypothesis that MEF2A is required in cardiac contractility employing zebrafish as a model organism. MEF2A is highly expressed in heart as well as somites during zebrafish embryogenesis. Knock-down of MEF2A in zebrafish impaires the cardiac contractility and results in sarcomere assembly defects. Dysregulation of cardiac genes in MEF2A morphants suggests that sarcomere assembly disturbances account for the cardiac contractile deficiency. Our studies suggested that MEF2A is essential in cardiac contractility.
Wang
Yue-Xiang
YX
Department of Molecular Genetics, Shanghai Medical School and Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, Shanghai 200032, PR China.
Qian
Lin-Xi
LX
Yu
Zhang
Z
Jiang
Qiu
Q
Dong
Yong-Xin
YX
Liu
Xue-Fei
XF
Yang
Xin-Ying
XY
Zhong
Tao P
TP
Song
Hou-Yan
HY
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D026161:MADS Domain Proteins; D064326:MEF2 Transcription Factors; D008845:Microinjections; D009024:Morphogenesis; D009200:Myocardial Contraction / Q000502:physiology*; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D018006:Myogenic Regulatory Factors; D016376:Oligonucleotides, Antisense; D010641:Phenotype; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000033:anatomy & histology / Q000196:embryology* / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D026161:MADS Domain Proteins; D064326:MEF2 Transcription Factors; C578035:MEF2A protein, human; D018006:Myogenic Regulatory Factors; D016376:Oligonucleotides, Antisense; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors