title: Ecotoxicological evaluation of diethanolamine using a battery of microbiotests.
abstract: In order to investigate the potential ecotoxicity of diethanolamine (DEA), a battery of model systems was developed. DEA is widely used as a chemical intermediate and as a surface-active agent in cosmetic formulations, pharmaceuticals and agricultural products. DEA was studied using ecotoxicological model systems, representing four trophic levels, with several bioindicators evaluated at different exposure time periods. The battery included bioluminescence inhibition of the bacterium Vibrio fischeri, growth inhibition of the alga Chlorella vulgaris and immobilization of the cladoceran Daphnia magna. Cell morphology, total protein content, neutral red uptake, MTS metabolization, lysosomal function, succinate dehydrogenase activity, G6PDH activity, metallothionein levels and EROD activity were studied in the hepatoma fish cell line PLHC-1, derived from Poeciliopsis lucida. The systems most sensitive to DEA were both D. magna and V. fischeri, followed by C. vulgaris and the fish cell line PLHC-1. The most prominent morphological effect observed in PLHC-1 cultures exposed to DEA was the induction of a marked steatosis, followed by death at high concentrations, in some cases by apoptosis. The main biochemical modification was a nearly three-fold increase in metallothionein levels, followed by the stimulations of lysosomal function and succinate dehydrogenase and G6PDH activities. Judging by the EC(50) values in the assay systems, DEA is not expected to produce acute toxic effects in the aquatic biota. However, chronic and synergistic effects with other chemicals cannot be excluded.
authors.affiliation: National Institute of Toxicology and Forensic Sciences, Av. Dr Fedriani s/n, 41009 Sevilla, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Zurita
authors.forename: G
authors.initials: G
authors.lastname: Repetto
authors.forename: A
authors.initials: A
authors.lastname: Jos
authors.forename: A
authors.initials: A
authors.lastname: Del Peso
authors.forename: M
authors.initials: M
authors.lastname: Salguero
authors.forename: M
authors.initials: M
authors.lastname: López-Artíguez
authors.forename: D
authors.initials: D
authors.lastname: Olano
authors.forename: A
authors.initials: A
authors.lastname: Cameán
mesh_terms: D048908:Aliivibrio fischeri / Q000187:drug effects / Q000502:physiology; D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048408:Chlorella vulgaris / Q000187:drug effects / Q000254:growth & development; D003621:Daphnia / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D017753:Ecosystem; D004983:Ethanolamines / Q000633:toxicity*; D005399:Fishes; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D049449:Luminescence; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D018675:Toxicity Tests
chemical_list: D004983:Ethanolamines; D008668:Metallothionein; C020283:diethanolamine; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase