title: Transthyretin-related proteins function to facilitate the hydrolysis of 5-hydroxyisourate, the end product of the uricase reaction.
abstract: Purine catabolic pathway in Bacillus subtilis is consisted of more than 14 genes. Among these genes, pucL and pucM are required for uricase activity. While PucL is known to encode the uricase itself, the function of PucM is still unclear although this protein is also indispensable for uric acid decomposition. Here, we provide evidence that PucM, a transthyretin-related protein, functions to facilitate the hydrolysis of 5-hydroxyisourate, the end product of the uricase reaction. Based on these results, we propose that transthyretin-related proteins present in diverse organisms are not functionally related to transthyretin but actually function as a hydroxyisourate hydrolase.
authors.affiliation: Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Oun-dong, Yuseong-gu, Daejeon 305-333, South Korea.
authors.forename: Youra
authors.initials: Y
authors.lastname: Lee
authors.forename: Do Hee
authors.initials: DH
authors.lastname: Lee
authors.forename: Chang Won
authors.initials: CW
authors.lastname: Kho
authors.forename: Ah Young
authors.initials: AY
authors.lastname: Lee
authors.forename: Mi
authors.initials: M
authors.lastname: Jang
authors.forename: Sayeon
authors.initials: S
authors.lastname: Cho
authors.forename: Choong Hwan
authors.initials: CH
authors.lastname: Lee
authors.forename: Jong Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Pyung Keun
authors.initials: PK
authors.lastname: Myung
authors.forename: Byoung Chul
authors.initials: BC
authors.lastname: Park
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011228:Prealbumin / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014503:Urate Oxidase / Q000378:metabolism*; D014527:Uric Acid / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011228:Prealbumin; D014527:Uric Acid; D014503:Urate Oxidase