Isolation and expression of FIP-2 in wounded pulp of the rat.
Pulpal wound healing followed by cavity preparation may involve reactionary or reparative dentinogenesis in relation to the cavity position; however, little is known about the molecular responses. We aimed to isolate and analyze genes induced or suppressed in the wounded pulp to identify molecular processes involved in the pulp responses to injury. Twenty-three cDNAs were isolated by cDNA subtraction between healthy and wounded pulp of rats. By library screening, we identified rat 14.7K-interacting protein (rFIP)-2A and B genes homologous to human FIP-2, being involved in regulating membrane trafficking and cellular morphogenesis. RT-PCR analysis showed induction for only rFIP-2B in the wounded pulp. In situ hybridization analysis revealed that both rFIP-2s were expressed strongly in condensing mesenchymal cells of the palatal process and surrounding Meckel's cartilage, but not in intramembranous chondrogenic cells. Thus, up-regulated rFIP-2B expression may play a role in regulating membrane trafficking or cellular morphogenesis of these embryonic and wounded pulpal cells.
Oyama
M
M
Department of Pathophysiology-Periodontal Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikatacho, Okayama, 700-8525, Japan.
Myokai
F
F
Ohira
T
T
Shiomi
N
N
Yamashiro
K
K
Arai
H
H
Nishimura
F
F
Takashiba
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D003782:Dental Pulp / Q000196:embryology / Q000293:injuries* / Q000378:metabolism; D003809:Dentin, Secondary / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D008648:Mesoderm / Q000378:metabolism; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D035582:Transcription Factor TFIIIA / Q000096:biosynthesis* / Q000302:isolation & purification; D015854:Up-Regulation; D014945:Wound Healing / Q000235:genetics*
D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; C504996:FIP-2B protein, rat; C000598853:OPTN protein, rat; D012333:RNA, Messenger; D035582:Transcription Factor TFIIIA