Supplementing a ruminally undegradable protein supplement to maintain essential amino acid supply to the small intestine when forage intake is restricted in beef cattle.
Twelve Angus crossbred cattle (eight heifers and four steers; average initial BW = 594 +/- 44.4 kg) fitted with ruminal and duodenal cannulas and fed restricted amounts of forage plus a ruminally undegradable protein (RUP) supplement were used in a triplicated 4 x 4 Latin square design experiment to determine intestinal supply of essential AA. Cattle were fed four different levels of chopped (2.54 cm) bromegrass hay (11.4% CP, 57% NDF; OM basis): 30, 55, 80, or 105% of the forage intake required for maintenance. Cattle fed below maintenance were given specified quantities of a RUP supplement (6.8% porcine blood meal, 24.5% hydrolyzed feather meal, and 68.7% menhaden fish meal; DM basis) designed to provide duodenal essential AA flow equal to that of cattle fed forage at 105% of maintenance. Experimental periods lasted 21 d (17 d of adaptation and 4 d of sampling). Total OM intake and duodenal OM flow increased linearly (P < 0.001) as cattle consumed more forage; however, OM truly digested in the rumen (% of intake) did not change (P = 0.43) as intake increased. True ruminal N degradation (% of intake) tended (P = 0.07) to increase linearly, and true ruminal N degradation (g/d) decreased quadratically (P = 0.02) as intake increased from 30 to 105%. Duodenal N flow was equal (P = 0.33) across intake levels, even though microbial N flow increased linearly (P < 0.001) as forage OM intake increased. Total and individual essential AA intake decreased (cubic; P < 0.001) as forage intake increased because the supply of nonammonia, nonmicrobial N flow from RUP was decreased (linear; P < 0.001) by design. Total duodenal flow of essential AA did not differ (P = 0.39) across these levels of forage intake. Although the profile of essential AA reaching the duodenum differed (P < or = 0.02) for all 10 essential AA, the range of each essential AA as a proportion of total essential AA was low (11.1 to 11.2% of total essential AA for phenylalanine to 12.3 to 14.3% of total essential AA for lysine). Duodenal essential AA flow did not differ (P = 0.10 to 0.65) with forage intake level for eight of the 10 essential AA. Duodenal flow of arginine decreased linearly (P = 0.01), whereas duodenal flow of tryptophan increased linearly (P = 0.002) as forage intake increased from 30 to 105% of maintenance. Balancing intestinal essential AA supply in beef cattle can be accomplished by varying intake of a RUP supplement.
Scholljegerdes
E J
EJ
Department of Animal Science, University of Wyoming, Laramie, 82071, USA.
Weston
T R
TR
Ludden
P A
PA
Hess
B W
BW
D000596:Amino Acids / Q000032:analysis / Q000378:metabolism*; D000641:Ammonia / Q000032:analysis; D000818:Animals; D031724:Bromus / Q000378:metabolism; D002417:Cattle / Q000378:metabolism*; D004044:Dietary Proteins / Q000008:administration & dosage / Q000378:metabolism*; D019587:Dietary Supplements; D004386:Duodenum / Q000737:chemistry; D005232:Fatty Acids, Volatile / Q000032:analysis; D005260:Female; D005285:Fermentation / Q000502:physiology; D005508:Food Deprivation / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D009584:Nitrogen / Q000378:metabolism; D011897:Random Allocation; D012417:Rumen / Q000378:metabolism / Q000382:microbiology
D000596:Amino Acids; D004044:Dietary Proteins; D005232:Fatty Acids, Volatile; D000641:Ammonia; D009584:Nitrogen