title: Lotus burttii takes a position of the third corner in the lotus molecular genetics triangle.
abstract: In order to consolidate molecular genetic system in Lotus japonicus and to further access the biological diversity in Lotea, we introduce here Lotus burttii B-303 derived from West Pakistan as the third crossing partner of the Gifu ecotype (B-129-S9) for a genetic analysis. L. burttii is a relatively small and early flowering plant with non-shattering behavior. The general chromosome morphology is very similar to Gifu, and fluorescence in situ hybridization (FISH) analysis revealed that the short arm of chromosome 1 in L. burttii is comparable to that of Gifu, indicating that the translocation event involving chromosomes 1 and 2, which was observed in L. japonicus Miyakojima MG-20, is not present in L. burttii. In addition L. burttii has a higher level of DNA polymorphism compared to Gifu and MG-20 enabling design of codominant markers such as SSR, CAPS and dCAPS. Using an F2 population from a cross between Gifu and L. burttii, codominant makers that co-segregated at the translocation site could be expanded. In order to normalize the genetic background, L. burttii was inbred for nine generations and the germplasm L. burttii B-303-S9 was established.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan. masayosi@biol.s.u-tokyo.ac.jp
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Andrea
authors.initials: A
authors.lastname: Pedrosa-Harand
authors.forename: Koji
authors.initials: K
authors.lastname: Yano
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Murooka
authors.forename: Katsuharu
authors.initials: K
authors.lastname: Saito
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Nagata
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Namai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishida
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shibata
authors.forename: Shusei
authors.initials: S
authors.lastname: Sato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
authors.forename: Masaki
authors.initials: M
authors.lastname: Hayashi
authors.forename: Kyuya
authors.initials: K
authors.lastname: Harada
authors.forename: Niels
authors.initials: N
authors.lastname: Sandal
authors.forename: Jens
authors.initials: J
authors.lastname: Stougaard
authors.forename: Andreas
authors.initials: A
authors.lastname: Bachmair
authors.forename: William F
authors.initials: WF
authors.lastname: Grant
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D000070116:Lotus / Q000145:classification / Q000235:genetics*; D008967:Molecular Biology; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D014178:Translocation, Genetic / Q000235:genetics
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers