[Study of the down-regulating effect of hepatitis C virus NS5A protein on NACA promoter].
OBJECTIVE
To investigate the effect of hepatitis C virus (HCV) non-structure protein NS5A on the activity of calcium-regulating protein alpha subunit of nascent polypeptide-associated complex (NACA) promoter.


METHODS
HepG2 cell plasmid pCAT3-NACA, containing NACA promoter, was transfected alone or cotransfected with pcDNA3.1(-)-NS5A, and chloramphenicol acetyl transferase (CAT) enzyme activity was assayed by enzyme-linked immunoassay (ELISA).


RESULTS
The CAT activity in the pcDNA3.1(-)-NS5A cotransfection group was 20.7% of the CAT activity in the pCAT3-NACA group.


CONCLUSIONS
HCV non-structural protein NS5A has a down-regulating effect on the promoter of NACA gene.
Dai
Jiu-zeng
JZ
Gene Therapy Research Center, Institute of Infectious Diseases, 302 Hospital of PLA, Beijing 100039, China. daijiuzeng18@163.com
Cheng
Jun
J
Qu
Jian-hui
JH
Ji
Dong
D
D001483:Base Sequence; D015536:Down-Regulation; D006801:Humans; D018832:Molecular Chaperones / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015533:Transcriptional Activation; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000494:pharmacology*; D012324:RNA-Dependent RNA Polymerase
D018832:Molecular Chaperones; D017361:Viral Nonstructural Proteins; C096099:nascent-polypeptide-associated complex; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase