Rapid and simple procedure for homogenizing leaf tissues suitable for mini-midi-scale DNA extraction in rice.
We describe a rapid and simple procedure for homogenizing leaf samples suitable for mini/midi-scale DNA preparation in rice. The methods used tungsten carbide beads and general vortexer for homogenizing leaf samples. In general, two samples can be ground completely within 11.3+/-1.5 sec at one time. Up to 20 samples can be ground at a time using a vortexer attachment. The yields of the DNA ranged from 2.2 to 7.6 microg from 25-150 mg of young fresh leaf tissue. The quality and quantity of DNA was compatible for most of PCR work and RFLP analysis.
Yi
Gihwan
G
Rice Division, Yeongnam Agricultural Research Institute, Milyang, Korea. gihwan@rda.go.kr
Choi
Jun-Ho
JH
Lee
Jong-Hee
JH
Jeong
Unggi
U
Nam
Min-Hee
MH
Yun
Doh-Won
DW
Eun
Moo-Young
MY
D018744:DNA, Plant / Q000302:isolation & purification*; D008722:Methods; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics; D017973:Tungsten Compounds / Q000737:chemistry
D018744:DNA, Plant; D017973:Tungsten Compounds; C002802:tungsten carbide