Fluorescence in situ hybridization sperm analysis of six translocation carriers provides evidence of an interchromosomal effect.
OBJECTIVE
To investigate the existence of an interchromosomal effect in balanced reciprocal translocation carriers and to evaluate their risk of having an affected child or repeated spontaneous abortions.


METHODS
Analysis of chromosomal sperm content by fluorescence in situ hybridization (FISH) using probes for chromosomes 1, 15, 16, 17, 18, X, and Y.


METHODS
University hospital.


METHODS
Six male carriers of balanced chromosome rearrangements, one with normal sperm parameters and five with oligoasthenoteratozoospermia, and seven fertile controls.


METHODS
Fluorescence in situ hybridization for chromosomal enumeration on sperm samples.


METHODS
Rate of disomy for the studied chromosomes.


RESULTS
A total of 123,842 spermatozoa were scored (82,181 for controls and 41,661 for patients). For each patient, at least one chromosome studied presented a significantly increased rate of disomy. One patient showed a clear interchromosomal effect for at least three of the six chromosomes studied. Disomy rates were statistically significantly and inversely correlated with the total progressive motility of spermatozoa.


CONCLUSIONS
The observed interchromosomal effect seems to be translocation, patient, and chromosome dependent. Variable effects were observed, according to the chromosome studied. When we looked at patients carrying the same translocation, the level of disomy rate was variable and affected different chromosomes.
Machev
Nadejda
N
Service de Biologie de la Reproduction, SIHCUS-CMCO, CHU de Strasbourg, Schiltigheim, France.
Gosset
Philippe
P
Warter
Stéphanie
S
Treger
Michèle
M
Schillinger
Monique
M
Viville
Stéphane
S
D000328:Adult; D016009:Chi-Square Distribution; D002874:Chromosome Mapping; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000235:genetics; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D014178:Translocation, Genetic / Q000235:genetics*; D024182:Uniparental Disomy