title: Optimizing incubation conditions for the preservation of sperm motility in processed semen samples.
abstract: To determine the optimal conditions to maintain sperm motility and viability for prolonged periods, semen specimens were processed and cultured in different temperature and conditions. We conclude that processing of semen and incubation of sperm in protein-supplemented N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) human tubal fluid medium at room temperature best preserved viability and motility of sperm.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Carolina
authors.initials: C
authors.lastname: Petrella
authors.forename: Jennifer
authors.initials: J
authors.lastname: Hsieh
authors.forename: Kim
authors.initials: K
authors.lastname: Thrift
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Jarow
authors.forename: Howard
authors.initials: H
authors.lastname: Zacur
authors.forename: Yulian
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000328:Adult; D002470:Cell Survival / Q000502:physiology*; D006801:Humans; D008297:Male; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D014021:Tissue Preservation / Q000379:methods*