title: Isolation of arboviruses from mosquitoes collected in northern Vietnam.
abstract: In response to recent increases in cases of pediatric encephalitis with unknown etiology in northern Vietnam, surveillance for arbovirus activity was conducted in four provinces surrounding the city of Hanoi during June 2002 and July-August 2004. A total of 20,615 mosquitoes consisting of 19 species in 1,122 pools were processed for virus isolation; virus isolates were obtained from 44 pools. Sagiyama virus (11 isolates), Getah virus (15 isolates), Oya virus (13 isolates), and Akabane virus (4 isolates) were identified by immunofluorescence assay and sequence analysis of reverse transcription-polymerase chain reaction fragments. Surprisingly, no isolates of Japanese encephalitis (JE) virus were obtained. Isolation of Akabane virus, Oya virus, Getah virus, and Sagiyama virus is reported for the first time from Vietnam.
authors.affiliation: Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA.
authors.forename: Juliet E
authors.initials: JE
authors.lastname: Bryant
authors.forename: Mary B
authors.initials: MB
authors.lastname: Crabtree
authors.forename: Vu Sin
authors.initials: VS
authors.lastname: Nam
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Yen
authors.forename: Hoang Minh
authors.initials: HM
authors.lastname: Duc
authors.forename: Barry R
authors.initials: BR
authors.lastname: Miller
mesh_terms: D000330:Aedes / Q000821:virology; D000524:Alphavirus / Q000302:isolation & purification; D000818:Animals; D000852:Anopheles / Q000821:virology; D001102:Arbovirus Infections / Q000453:epidemiology / Q000821:virology; D001103:Arboviruses / Q000302:isolation & purification*; D002043:Bunyaviridae / Q000302:isolation & purification; D003465:Culex / Q000821:virology; D009033:Culicidae / Q000145:classification / Q000821:virology*; D018792:Encephalitis, Viral / Q000453:epidemiology / Q000821:virology; D012398:Ross River virus / Q000302:isolation & purification; D012621:Seasons; D014744:Vietnam / Q000453:epidemiology