Hsp70 does not mediate the hyperthermia-associated hyporesponsiveness to angiotensin in isolated rat portal veins.
In rat portal veins (RPV) isolated from septic rats, we previously showed that the contractile response to angiotensin II (AT(II)) was significantly decreased and that the vascular failure was correlated with the severity of the disease. We hypothesized that hyperthermia might be one of the factors responsible for the vascular failure. Moreover, hyperthermia should concomitantly increase heat shock proteins (Hsps) expression. We then compared the vascular contractility and the heat shock protein 70 (Hsp70) expression in RPV incubated at 37 degrees C and 39.5 degrees C and sought for a relationship between both events. In our experimental model, hyperthermia increased the Hsp70 expression and decreased the contractile response to AT(II). Incorporation of the Hsp70 antisense oligonucleotide in RPV blocked the increase in Hsp70 expression but had no consequence on the contractile response to AT(II). In conclusion, hyperthermia increases Hsp70 expression but does not mediate the decreased response to AT(II). Hsp70 overexpression has no effect on the actin-myosin interaction in vascular smooth muscle.
Frossard
Jean-Louis
JL
Division de Gastroentérologie, Hôpitaux Universitaires de Genève, Geneva, Switzerland.
Mastrangelo
Dominique
D
Hadengue
Antoine
A
Pastor
Catherine M
CM
D000809:Angiotensins / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D005334:Fever / Q000503:physiopathology*; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D011169:Portal Vein / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D000809:Angiotensins; D018840:HSP70 Heat-Shock Proteins; D016376:Oligonucleotides, Antisense; D014662:Vasoconstrictor Agents