Direct high-performance liquid chromatographic analysis of D-tocopheryl acid succinate and derivatives.
A method of analysis of a Vitamin E derivative D-tocopheryl acid succinate (TS) in biological fluids and commercially available products is necessary to study the kinetics of in vitro and in vivo metabolism, tissue distribution, and content uniformity. A simple and inexpensive high-performance liquid chromatographic method was developed for the direct determination of D-tocopheryl acid succinate in commercially available products, rat serum, and rat tissues. This method can also be applied to the determination of 15 Vitamin E derivatives. Rat serum (0.1 ml) was extracted with sodium dodecyl sulfate, ethanol, hexane, and then dried under nitrogen gas after addition of the internal standard, DL-alpha-tocopherol acetate. Separation was achieved on a C18 column with UV detection at 205 nm. The calibration curve for D-tocopheryl acid succinate was linear ranging from 0.025 to 100 microg/ml. The mean extraction efficiency was >92%. Precision of the assay was <5% (CV), and was within 5% at the limit of quantitation (0.025 microg/ml). Bias of the assay was lower than 5%, and was within 5% at the limit of quantitation. The assay was applied successfully to the serum and tissue distribution of D-tocopheryl acid succinate in rats, various Vitamin E derivatives, and content uniformity in commercially available products containing D-tocopheryl acid succinate.
Good
Renee L
RL
College of Pharmacy Department of Pharmaceutical Sciences and Pharmacotherapy, Washington State Washington, Pullman, 99164-6534, USA.
Roupe
Kathryn A
KA
Fukuda
Chie
C
Clifton
G Dennis
GD
Fariss
Marc W
MW
Davies
Neal M
NM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014018:Tissue Distribution; D024505:Tocopherols; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics
D014810:Vitamin E; D024505:Tocopherols