Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass.
We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor alpha gene expressions were upregulated in SV-T2 and were thought to be candidates for call surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.
Tuoya
Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
Hirayama
Koichi
K
Nagaoka
Tadahiro
T
Yu
Dongwei
D
Fukuda
Takayuki
T
Tada
Hiroko
H
Yamada
Hidenori
H
Seno
Masaharu
M
D000818:Animals; D041702:BALB 3T3 Cells; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
D015415:Biomarkers; D008565:Membrane Proteins