[High-performance liquid chromatographic determination of propanidid and etomidate].
High-performance reversed-phase liquid chromatographic determination of propanidid and etomidate was performed on ODS silica, after precipitation of the proteins in plasma with methanol, extraction of the drugs with diethyl ether, evaporation and dissolving in a mobile phase: acetonitrile-phosphate buffer pH 4.44 (7:3, v/v); UV detection at 254 nm. Dionine hydrochloride was used as an internal standard. Propanidid was determined in a range 5-25 microg/cm3 of plasma and etomidate in 0.1-0.5 microg/cm3 of plasma, thus enables the analysis of therapeutic levels of the drugs.
Zawisza
P
P
Akademia Medyczna, ul. Okopowa 1, 20-022 Lublin.
Przyborowski
L
L
D018686:Anesthetics, Intravenous / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005045:Etomidate / Q000097:blood*; D006801:Humans; D011410:Propanidid / Q000097:blood*
D018686:Anesthetics, Intravenous; D011410:Propanidid; D005045:Etomidate