title: Progenitor cell injury after irradiation to the developing brain can be modulated by mild hypothermia or hyperthermia.
abstract: Ionizing radiation induced acute cell death in the dentate gyrus subgranular zone (SGZ) and the subventricular zone (SVZ). Hypomyelination was also observed. The effects of mild hypothermia and hyperthermia for 4 h after irradiation (IR) were studied in postnatal day 9 rats. One hemisphere was irradiated with a single dose of 8 Gy and animals were randomized to normothermia (rectal temperature 36 degrees C for 4 h), hypothermia (32 degrees C for 4 h) or hyperthermia (39 degrees C for 4 h). Cellular injury, e.g. chromatin condensation and nitrotyrosine formation, appeared to proceed faster when the body temperature was higher. Caspase-3 activation was more pronounced in the hyperthermia group and nuclear translocation of p53 was less pronounced in the hypothermia group 6 h after IR. In the SVZ the loss of nestin-positive progenitors was more pronounced (48%) and the size was smaller (45%) in the hyperthermia group 7 days post-IR. Myelination was not different after hypo- or hyperthermia. This is the first report to demonstrate that hypothermia may be beneficial and that hyperthermia may aggravate the adverse side-effects after radiation therapy to the developing brain.
authors.affiliation: The Arvid Carlsson Institute of Neuroscience at the Institute of Clinical Neuroscience, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden.
authors.forename: Aya
authors.initials: A
authors.lastname: Fukuda
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Fukuda
authors.forename: Marie
authors.initials: M
authors.lastname: Jönsson
authors.forename: Janos
authors.initials: J
authors.lastname: Swanpalmer
authors.forename: Sven
authors.initials: S
authors.lastname: Hertzman
authors.forename: Birgitta
authors.initials: B
authors.lastname: Lannering
authors.forename: Thomas
authors.initials: T
authors.lastname: Björk-Eriksson
authors.forename: Ildikó
authors.initials: I
authors.lastname: Màrky
authors.forename: Klas
authors.initials: K
authors.lastname: Blomgren
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects; D001831:Body Temperature / Q000502:physiology*; D001921:Brain / Q000254:growth & development / Q000503:physiopathology / Q000528:radiation effects*; D001925:Brain Damage, Chronic / Q000503:physiopathology / Q000517:prevention & control / Q000628:therapy*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D018891:Dentate Gyrus / Q000254:growth & development / Q000503:physiopathology / Q000528:radiation effects; D005260:Female; D006979:Hyperthermia, Induced / Q000009:adverse effects; D007036:Hypothermia, Induced; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008297:Male; D009410:Nerve Degeneration / Q000503:physiopathology / Q000517:prevention & control / Q000628:therapy; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000502:physiology / Q000528:radiation effects*; D011833:Radiation Injuries, Experimental / Q000503:physiopathology / Q000517:prevention & control / Q000628:therapy*; D011839:Radiation, Ionizing; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000502:physiology / Q000528:radiation effects*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D016159:Tumor Suppressor Protein p53; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases