[Effect of flavonoids of ginkgo biloba on anti-oxidizing system of mice after acute alcohol administration].
OBJECTIVE
To investigate the protective effects of flavonoids of ginkgo biloba on anti-oxidizing system damaged by acute alcohol administration.


METHODS
Adult male Kunming mice were employed and divided into randomly flavonoid intervention group, normal control and ethanol control group according to body weight. After pretreated with flavonoids of ginkgo biloba (96mg/kg bw), the mice in flavonoid intervention group ingested alcohol (ethanol 4.8g/kg bw) via i.g. and were decapitated after 0.5, 1, 2, 4, 6, 9, and 15 h of ethanol administration. The same treatment was carried out on ethanol control group except that physiological saline was applied instead of flavonoid of ginkgo biloba. Meanwhile, the normal control group was established.


RESULTS
The concentration of glutathione (GSH), malondialdehyde (MDA) and the activity of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) in the serum and liver were determined. The experiment displays that the content of GSH and the activities of GSH-Px and SOD decreased rapidly after 1 h of treatment with alcohol and dropped to the lowest level at 4h of treatment. After 6h of treatment, these indexes came to the normal level rapidly. The level of MDA of serum and liver increased rapidly after 1 h of treatment and reached the climax at 4h and 6h respectively. It went back to the normal concentration until 15h and 9 h respectively. On a whole, there were similar curves between flavonoids intervention group and alcohol control group on the indexes. However, to some extent, the supplement of flavonoid of ginkgo biloba can prohibit the rise of MDA level and the decline of GSH-Px, SOD, GSH which were induced by acute alcohol intakes.


CONCLUSIONS
Flavonoid of ginkgo biloba have some protective effects on the damage of anti-oxidizing system of mice induced by acute alcohol adminstration.
Yao
Ping
P
School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Liu
Lie-Gang
LG
Jia
Wen-Bo
WB
Song
Fang-Fang
FF
Zhou
Shaoliang
S
Zhang
Xiping
X
Sun
Xiufa
X
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D000431:Ethanol / Q000633:toxicity*; D005419:Flavonoids / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry*; D008297:Male; D051379:Mice; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D005419:Flavonoids; D000431:Ethanol; D013482:Superoxide Dismutase