B-Cells in ocular adnexal lymphoproliferative lesions express B-cell attracting chemokine 1 (CXCL13).
OBJECTIVE
The homeostatic chemokine, B cell attracting chemokine 1 (CXCL13), has been implicated in the pathogenesis of lymphocyte-mediated diseases. We investigated the cellular expression of this chemokine in the spectrum of ocular adnexal lymphoproliferative lesions.


METHODS
Laboratory investigation.


METHODS
CXCL13 expression in paraffin-embedded adnexal biopsy specimens was determined by indirect immunohistochemistry with antigen retrieval.


RESULTS
In 15 of 16 biopsy specimens, including reactive lymphoid hyperplasia (n = 7), atypical lymphoid hyperplasia (n = 3), and B cell lymphoma (n = 6), CXCL13 was detected. CD20-positive B-cells, as well as dendritic cells and endothelial cells, expressed the chemokine.


CONCLUSIONS
B-cells in ocular adnexal lymphoproliferative lesions demonstrate expression of CXCL13, a chemokine that may participate in tumor pathogenesis and is a potential target for novel therapies.
Falkenhagen
Katherine M
KM
Casey Eye Institute, Oregon Health & Science University, Portland, Oregon 97239-4197, USA.
Braziel
Rita M
RM
Fraunfelder
Frederick W
FW
Smith
Justine R
JR
D000911:Antibodies, Monoclonal; D018951:Antigens, CD20 / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism*; D001706:Biopsy; D054382:Chemokine CXCL13; D019743:Chemokines, CXC / Q000378:metabolism*; D003229:Conjunctival Diseases / Q000378:metabolism / Q000473:pathology; D003230:Conjunctival Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000378:metabolism* / Q000473:pathology; D019310:Pseudolymphoma / Q000378:metabolism* / Q000473:pathology
D000911:Antibodies, Monoclonal; D018951:Antigens, CD20; C110663:CXCL13 protein, human; D054382:Chemokine CXCL13; D019743:Chemokines, CXC