title: Denaturing high-performance liquid chromatography for mutation detection and genotyping.
abstract: Denaturing high-performance liquid chromatography (DHPLC) is an accurate and efficient screening technique used for detecting deoxyribonucleic acid sequence changes by heteroduplex analysis. It can also be used for genotyping of single-nucleotide polymorphisms. The high-sensitivity of DHPLC has made this technique one of the most reliable approaches to mutation analysis and is used in various areas of genetics, both in the research and clinical arena. This chapter describes the methods used for mutation detection analysis and the genotyping of single-nucleotide polymorphisms by DHPLC on the WAVETM system from Transgenomic Inc.
authors.affiliation: Department of Human Genetics, The University of Chicago, Chicago, IL, USA.
authors.forename: Donna Lee
authors.initials: DL
authors.lastname: Fackenthal
authors.forename: Pei Xian
authors.initials: PX
authors.lastname: Chen
authors.forename: Soma
authors.initials: S
authors.lastname: Das
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004247:DNA / Q000032:analysis*; D004252:DNA Mutational Analysis / Q000379:methods*; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D009154:Mutation / Q000235:genetics*
chemical_list: D004247:DNA