title: Characterization of hydroxyaromatic compounds in vegetable oils by capillary electrophoresis with direct injection in an oil-miscible KOH/propanol/methanol medium.
abstract: The separation of hydroxyaromatic compounds in vegetable oils, including synthetic antioxidants (3-tert-butyl-4-hydroxyanisol and 2,6-di-tert-butyl-4-hydroxytoluene), E-vitamers and other natural oil components, by nonaqueous capillary electrophoresis in an oil-miscible background electrolyte (BGE) was investigated. The BGE contained 40 mM KOH in a methanol/1-propanol (PrOH) mixture (15:85 v/v). The oil samples were 1:1 diluted with PrOH and directly injected in the capillary. Under negative polarity (cathode at the injection end), the anionic solutes moved faster than the electroosmotic flow, being well-resolved among them and from the triacylglycerols. Using virgin palm, extra virgin olive, wheat germ, virgin soybean and other oils, the capability of the procedure to quickly yield a characteristic profile of the biophenols present in the sample was demonstrated. The alpha-, (beta + gamma)- (as unresolved pair) and delta-tocopherols of a soybean oil sample were quantified.
authors.affiliation: Departament de Química Analítica, Universitat de València, Burjassot, Spain.
authors.forename: Carla R B
authors.initials: CR
authors.lastname: Mendonça
authors.forename: Clara I D
authors.initials: CI
authors.lastname: Bica
authors.forename: Clarisse M S
authors.initials: CM
authors.lastname: Piatnicki
authors.forename: Ernesto F
authors.initials: EF
authors.lastname: Simó-Alfonso
authors.forename: Guillermo
authors.initials: G
authors.lastname: Ramis-Ramos
mesh_terms: D000433:1-Propanol / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006878:Hydroxides / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*; D012995:Solubility
chemical_list: D006878:Hydroxides; D010636:Phenols; D010938:Plant Oils; D017680:Potassium Compounds; D000433:1-Propanol; C029943:potassium hydroxide; D000432:Methanol