title: A new method for the analysis of amikacin using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization and high-performance liquid chromatography with UV-detection.
abstract: A simple and sensitive reversed-phase liquid chromatographic method has been developed for the determination of amikacin (AMK) by derivatization. The method is based on the pre-column derivatization of AMK with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). The derivatization reaction proceeds in aqueous solution at room temperature with a borate buffer of pH 8.0. The formation of the corresponding derivative of AMK is instantaneous and it is stable for more than 36 h. Detection was performed by UV-absorption instead of fluorescence. Several factors influencing the derivatization reaction yields were studied and optimized. The system offered the following analytical parameters: limit of detection (LOD) of 0.068 micro g ml(-1) (3sigma), linear correlation coefficient of 0.9998 and linear range response from 2 to 50 microg ml(-1). The precision of the method was <1%. As a preliminary application, the method has been successfully applied to the amikacin determination in parenteral pharmaceutical formulations.
authors.affiliation: Analysis and Quality Control Department, Faculty of Pharmacy, University of Los Andes, Venezuela.
authors.forename: José F
authors.initials: JF
authors.lastname: Ovalles
authors.forename: María Del Rosario
authors.initials: Mdel R
authors.lastname: Brunetto
authors.forename: Máximo
authors.initials: M
authors.lastname: Gallignani
mesh_terms: D000583:Amikacin / Q000032:analysis*; D000634:Aminoquinolines / Q000737:chemistry*; D002219:Carbamates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: C078178:6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; D000634:Aminoquinolines; D002219:Carbamates; D000583:Amikacin