title: Solar-simulated ultraviolet radiation induces abnormal maturation and defective chemotaxis of dendritic cells.
abstract: Exposure to ultraviolet (UV) light induces immunosuppression. Different evidences indicate that this phenomenon is mainly a consequence of the effect of UV light on skin dendritic cells (DC). To investigate the cellular and molecular basis of this type of immunosuppression, we assessed in vitro the effect of solar-simulated UV radiation on the phenotypic and functional characteristics of human monocyte-derived DC and Langerhans-like DC. UV radiation induced a decreased expression of molecules involved in antigen capture as DC-SIGN and the mannose receptor. This effect was accompanied by a diminished endocytic capacity, an enhanced expression of molecules involved in antigen presentation such as major histocompatibility complex-II and CD86, and a significant increase in their capability to stimulate T cells. Furthermore, irradiated DC failed to acquire a full mature phenotype upon treatment with lipopolysaccharide. On the other hand, solar-simulated radiation induced the secretion of tumor necrosis factor-alpha and interleukin (IL)-10 by DC, but no IL-12. Interestingly, solar-simulated UV radiation also caused an altered migratory phenotype, with an increased expression of CXCR4, and a lack of induction of CCR7, thus correlating with a high chemotactic response to stromal cell-derived factor 1(SDF-1) (CXCL12), but not to secondary lymphoid tissue chemokine (SLC) (CCL21). These data indicate that solar-simulated UV radiation induces a defective maturation and an anomalous migratory phenotype of DC.
authors.affiliation: Servicio de Inmunología, Hospital de la Princesa Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Mittelbrunn
authors.forename: Reyes
authors.initials: R
authors.lastname: Tejedor
authors.forename: Hortensia
authors.initials: H
authors.lastname: de la Fuente
authors.forename: M Angeles
authors.initials: MA
authors.lastname: García-López
authors.forename: Angeles
authors.initials: A
authors.lastname: Ursa
authors.forename: Pablo F
authors.initials: PF
authors.lastname: Peñas
authors.forename: Amaro
authors.initials: A
authors.lastname: García-Díez
authors.forename: José Luis
authors.initials: JL
authors.lastname: Alonso-Lebrero
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Pivel
authors.forename: Salvador
authors.initials: S
authors.lastname: González
authors.forename: Roberto
authors.initials: R
authors.lastname: Gonzalez-Amaro
authors.forename: Francisco
authors.initials: F
authors.lastname: Sánchez-Madrid
mesh_terms: D017209:Apoptosis / Q000276:immunology / Q000528:radiation effects; D002450:Cell Communication / Q000276:immunology / Q000528:radiation effects; D002454:Cell Differentiation / Q000276:immunology / Q000528:radiation effects; D002634:Chemotaxis, Leukocyte / Q000276:immunology / Q000528:radiation effects*; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D009000:Monocytes / Q000166:cytology; D013472:Sunlight / Q000009:adverse effects; D013601:T-Lymphocytes / Q000166:cytology; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D016207:Cytokines