[Research on cytokines gene express of rats alcoholic liver disease affected by tea polyphenol].
OBJECTIVE
To evaluate the effect of tea polyphenol(TP) on the rat with alcoholic liver damage.


METHODS
Rats were divided into 3 groups, in which 2 groups were stomach perfused with alcohol to result in ALD, and 1 group of them stomach perfused with TP simultaneously. Another group was normal control groups (stomach perfused with drinking water). In the end of 12 weeks, the liver specimen of each rat was observed by anglicizing its tissue damage, and all data collected was performed by statistical analysis in quantum and semi-quantum. Meanwhile cytokines gene express of each group is determined.


RESULTS
In the end of 12 weeks, alcoholic hepatitis appeared in rat liver. Hepatic injury in alcohol group and TP group were found, but could not be found in normal group. Compared with pure alcohol group, alcoholic liver damage mainly showing with steatosis in TP group were slight, in addition showing liver cellular swelling with small area, with less spot and focal necrosis, none bridging necrosis. Steatosis were slight relatively, mega-bubble steatosis were less found. Collagen deposition of TP group were less than those of pure alcohol group. Gene expression of. cytokine have diversity statistically such as IL-3, IL-4, IL-1R2, IL-6R, IL-7R2, IL-3Ra, IL-R1, IL-13, IL-1R1, IL-7R2, EPO-R, LIFR, IL-1R2, IL-5R2, CSF1, CD27, IL-6R.


CONCLUSIONS
TP is able to attenuate alcoholic liver damage. It's mechanism is possibly due to modulating cytokines gene expression of cytokine.
Zhang
Xing-Guo
XG
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. ZXG777@sohu.com
Yu
Chao-Hui
CH
Jiang
Qi
Q
Zhang
Yu
Y
Chen
Shao-Hua
SH
Lu
You-Ming
YM
D000818:Animals; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D015870:Gene Expression; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D059808:Polyphenols; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018123:Receptors, Interleukin / Q000096:biosynthesis* / Q000235:genetics; D013662:Tea / Q000737:chemistry
D005419:Flavonoids; D007378:Interleukins; D010636:Phenols; D059808:Polyphenols; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D013662:Tea