title: IgE- and FcepsilonRI-mediated migration of human basophils.
abstract: Local accumulation of basophils at inflammatory sites is observed in experimental antigen challenge and in allergic diseases. It is not fully known what factor(s) regulates local basophil influx in tissues, and it has not been determined whether antigens belong in a panel of basophil chemoattractants. This study was designed to elucidate whether IgE- and high-affinity receptor for IgE (FcepsilonRI)-mediated stimulation can induce human basophil migration. The migration-inducing potency of an anti-FcepsilonRI alpha-chain mAb, CRA-1, was examined on human basophils. CRA-1 mAb elicited significant migration of basophils. The migration-inducing potency of this mAb was maximal at 100 ng ml-1, and CRA-1 mAb at 100 ng ml-1 attracted approximately 10% of total inoculated basophils above baseline levels after incubation for 2.5 h. Checkerboard analysis indicated that basophil migration induced by this mAb was mainly chemotactic and partially chemokinetic. An antigen, Der f 2, also induced migration of basophils from Der f-sensitive subjects. Basophils mixed with 1 ng ml-1 of CRA-1 mAb showed an exaggerated migration response to eotaxin, indicating that FcepsilonRI cross-linkage enhances basophil migration to other chemoattractants. Induction of basophil migration by IgE- and FcepsilonRI-cross-linking stimulation may, at least in part, explain the pathogenesis of local basophil accumulation clinically observed in allergic diseases such as asthma.
authors.affiliation: Department of Allergy and Rheumatology and.
authors.forename: Maho
authors.initials: M
authors.lastname: Suzukawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Hirai
authors.forename: Motoyasu
authors.initials: M
authors.lastname: Iikura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nagase
authors.forename: Akiko
authors.initials: A
authors.lastname: Komiya
authors.forename: Chitose
authors.initials: C
authors.lastname: Yoshimura-Uchiyama
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yamada
authors.forename: Chisei
authors.initials: C
authors.lastname: Ra
authors.forename: Ken
authors.initials: K
authors.lastname: Ohta
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Masao
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D039741:Antigens, Dermatophagoides / Q000494:pharmacology*; D060829:Arthropod Proteins; D001491:Basophils / Q000166:cytology / Q000276:immunology*; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology* / Q000494:pharmacology; D017455:Receptors, IgE / Q000276:immunology*
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451160:Dermatophagoides farinae antigen f 2; D017455:Receptors, IgE; D007073:Immunoglobulin E