title: A novel neisserial shuttle plasmid: a useful new tool for meningococcal research.
abstract: We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram-negative bacterium Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from Acinetobacter baumannii and pNI10 from Pseudomonas. Modified by the incorporation of a kanamycin resistance cassette, the plasmid can be stably maintained in Escherichia coli and Neisseria meningitidis, and can be used as a shuttle plasmid in meningococcal research.
authors.affiliation: Molecular Infectious Diseases Group, Department of Paediatrics, Imperial College London, Norfolk Place, London W2 1PG, UK. c.odwyer@imperial.ac.uk
authors.forename: Clíona A
authors.initials: CA
authors.lastname: O' Dwyer
authors.forename: Paul R
authors.initials: PR
authors.lastname: Langford
authors.forename: J Simon
authors.initials: JS
authors.lastname: Kroll
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005819:Genetic Markers; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D007613:Kanamycin Resistance / Q000235:genetics; D008969:Molecular Sequence Data; D009343:Neisseria / Q000235:genetics*; D009345:Neisseria meningitidis / Q000235:genetics*; D016366:Open Reading Frames; D010957:Plasmids / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005819:Genetic Markers