title: The Drosophila ACP65A cuticle gene: deletion scanning analysis of cis-regulatory sequences and regulation by DHR38.
abstract: The regulatory sequences of the Drosophila ACP65A cuticle gene were analyzed in vivo in transgenic flies, using both fusion genes constructs and transposase-mediated deletions within a P element containing ACP65A regulatory sequences fused to the lacZ gene (deletion scanning). The sequences located between -594 and +161 are sufficient to confer both temporal and spatial expression specificities, indicating the presence of tissue-specific enhancers and response elements to hormone-induced factors. In addition, timing of expression and tissue-specificity appear to be controlled by distinct cis-regulatory elements, which suggests the existence of independent hormonal and tissue-specific signaling pathways. Gain and loss of function studies also implicate DHR38, the Drosophila homolog of the vertebrate NGFI-B-type nuclear receptors, as an important activator of the ACP65A gene.
authors.affiliation: UMR CNRS 5548 Développement-Communication Chimique, Université de Bourgogne, Faculté des Sciences Gabriel, Dijon, France.
authors.forename: N
authors.initials: N
authors.lastname: Bruey-Sedano
authors.forename: J
authors.initials: J
authors.lastname: Alabouvette
authors.forename: M
authors.initials: M
authors.lastname: Lestradet
authors.forename: L
authors.initials: L
authors.lastname: Hong
authors.forename: A
authors.initials: A
authors.lastname: Girard
authors.forename: E
authors.initials: E
authors.lastname: Gervasio
authors.forename: B
authors.initials: B
authors.lastname: Quennedey
authors.forename: J P
authors.initials: JP
authors.lastname: Charles
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000502:physiology*; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D011679:Pupa / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology*; D011987:Receptors, Steroid / Q000502:physiology; D017384:Sequence Deletion; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C095176:Hr38 protein, Drosophila; D019476:Insect Proteins; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D014157:Transcription Factors; C047663:cuticle proteins, insects