title: Validation of a new method to measure hydrogen peroxide in exhaled breath condensate.
abstract: Inflammatory processes in the lung can or will elicit oxidative stress. The degree of oxidative stress can be determined by measuring hydrogen peroxide (H(2)O(2)) concentration in exhaled breath condensate (EBC) but the methods to measure H(2)O(2) are all rather time consuming and only reliable and/or accurate in the hand of skilled technicians in a dedicated laboratory. We tested a new commercial device (Ecocheck), developed to offer a less time-consuming method to measure H(2)O(2). We validated this new method according the NCCLS EP10-A2 protocol. The validation shows that the imprecision in the low range is high (28.4%) and declines with higher concentrations of H(2)O(2) (up to 6.6%). The Ecocheck is "an easy to use" measuring device for routine measurements getting quick results without the need for skilled technicians to determine H(2)O(2) concentrations.
authors.affiliation: Department of Clinical Chemistry, St. Antonius Hospital, PO Box 2500, NL-3430 EM Nieuwegein, The Netherlands. icb75097@nifty.com
authors.forename: Wim B
authors.initials: WB
authors.lastname: Gerritsen
authors.forename: Pieter
authors.initials: P
authors.lastname: Zanen
authors.forename: Anja A
authors.initials: AA
authors.lastname: Bauwens
authors.forename: Jules M
authors.initials: JM
authors.lastname: van den Bosch
authors.forename: Fred J
authors.initials: FJ
authors.lastname: Haas
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques; D001944:Breath Tests / Q000295:instrumentation / Q000379:methods*; D045853:Exhalation; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis; D015203:Reproducibility of Results; D012907:Smoking / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D015415:Biomarkers; D006861:Hydrogen Peroxide