Association of GST genotypes with age of onset and lymph node metastasis in oral squamous cell carcinoma.
BACKGROUND
Environment-gene interaction in oral carcinogenesis is well demonstrated by phase I and II enzymes that are involved in the metabolism of carcinogens. This study investigated the association of glutathione S-transferase (GST)T1 and GSTM1 genotypes of phase II enzyme genes with risk for, age of onset, and neck lymph node metastasis (LNM) in areca-associated oral squamous cell carcinoma (OSCC).


METHODS
A total of 114 OSCC male patients and 100 male controls were recruited. All subjects were areca users and tobacco smokers. DNA was obtained from peripheral blood samples. Genotyping of GSTT1 (non-null/null) and GSTM1 (non-null/null) was determined by polymerase chain reaction (PCR) analysis using specific primers that only amplify non-null alleles.


RESULTS
No association was found between GST genotype and the risk of OSCC based on case-controls. Patients with the GSTT1 null genotype were older at onset (P = 0.03). Those with the GSTM1 null genotype had a higher incidence of neck LNM than those with the GSTM1 non-null genotype (P = 0.01). Patients with the GSTM1/GSTT1 null genotype appeared to have later onset and a higher incidence of neck LNM than those carrying the opposite genotype.


CONCLUSIONS
The GST genotypes may be important markers for the age of onset and risk of metastasis in OSCC. The data also suggest that the various GST isoforms may be differentially involved in development or progression of OSCC.
Liu
Chung-Ji
CJ
Department of Oral and Maxillofacial Surgery, Mackay Memorial Hospital, Taipei, Taiwan.
Chang
Che-Shoa
CS
Lui
Man-Tin
MT
Dang
Ching-Wen
CW
Shih
Yin-Hua
YH
Chang
Kuo-Wei
KW
D000328:Adult; D017668:Age of Onset; D000368:Aged; D001114:Areca / Q000009:adverse effects; D002273:Carcinogens / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005787:Gene Frequency; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008658:Inactivation, Metabolic; D016015:Logistic Models; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000201:enzymology / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D016017:Odds Ratio; D020033:Protein Isoforms
D002273:Carcinogens; D005982:Glutathione Transferase; D020033:Protein Isoforms; C413545:glutathione S-transferase T1