title: Global changes of chlorophyll excitonic interactions in photosystem II during thermal denaturation.
abstract: Photosystem II (PSII) is a multisubunit chlorophyll-binding enzyme that absorbs light to catalyze water oxidation and plastoquinone reduction. Chlorophyll excitonic interaction changes in PSII were studied by absorption and circular dichroism spectra from 25 degrees C to 80 degrees C, and protein subunit denaturation was monitored by differential scanning calorimetry. A four-stage process of chlorophyll excitonic interaction change was observed being correlated with the denaturation of protein subunits.
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Ding
authors.forename: Yandao
authors.initials: Y
authors.lastname: Gong
authors.forename: Tingyun
authors.initials: T
authors.lastname: Kuang
authors.forename: Nanming
authors.initials: N
authors.lastname: Zhao
mesh_terms: D002734:Chlorophyll / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism*; D011489:Protein Denaturation; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D013057:Spectrum Analysis; D018724:Spinacia oleracea / Q000166:cytology / Q000378:metabolism; D013696:Temperature
chemical_list: D045332:Photosystem II Protein Complex; D021122:Protein Subunits; D002734:Chlorophyll