title: Quantitative trait locus (QTL) isogenic recombinant analysis: a method for high-resolution mapping of QTL within a single population.
abstract: In the quest for fine mapping quantitative trait loci (QTL) at a subcentimorgan scale, several methods that involve the construction of inbred lines and the generation of large progenies of such inbred lines have been developed (Complex Trait Consortium 2003). Here we present an alternative method that significantly speeds up QTL fine mapping by using one segregating population. As a first step, a rough mapping analysis is performed on a small part of the population. Once the QTL have been mapped to a chromosomal interval by standard procedures, a large population of 1000 plants or more is analyzed with markers flanking the defined QTL to select QTL isogenic recombinants (QIRs). QIRs bear a recombination event in the QTL interval of interest, while other QTL have the same homozygous genotype. Only these QIRs are subsequently phenotyped to fine map the QTL. By focusing at an early stage on the informative individuals in the population only, the efforts in population genotyping and phenotyping are significantly reduced as compared to prior methods. The principles of this approach are demonstrated by fine mapping an erucic acid QTL of rapeseed at a subcentimorgan scale.
authors.affiliation: Keygene N.V., NL-6708 PW, Wageningen, The Netherlands. johan.peleman@keygene.com
authors.forename: Johan D
authors.initials: JD
authors.lastname: Peleman
authors.forename: Crispin
authors.initials: C
authors.lastname: Wye
authors.forename: Jan
authors.initials: J
authors.lastname: Zethof
authors.forename: Anker P
authors.initials: AP
authors.lastname: Sørensen
authors.forename: Henk
authors.initials: H
authors.lastname: Verbakel
authors.forename: Jan
authors.initials: J
authors.lastname: van Oeveren
authors.forename: Tom
authors.initials: T
authors.lastname: Gerats
authors.forename: Jeroen Rouppe
authors.initials: JR
authors.lastname: van der Voort
mesh_terms: D029741:Brassica rapa / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping / Q000706:statistics & numerical data*; D004883:Erucic Acids / Q000378:metabolism; D005819:Genetic Markers; D005828:Genetics, Population / Q000706:statistics & numerical data; D040641:Quantitative Trait Loci; D011995:Recombination, Genetic; D018401:Sample Size
chemical_list: D004883:Erucic Acids; D005819:Genetic Markers; C049811:erucic acid