Mitochondria and calcium signaling.
The kinetic properties for the uptake, storage and release of Ca2+ from isolated mitochondria accurately predict the behaviour of the organelles within the intact cell. While the steady-state cycling of Ca2+ across the inner membrane between independent uptake and efflux pathways seems at first sight to be symmetrical, the distinctive kinetics of the uniporter, which is highly dependent on external free Ca2+ concentration and the efflux pathway, whose activity is clamped over a wide range of total matrix Ca2+ by the solubility of the calcium phosphate complex provide a mechanism whereby mitochondria reversibly sequester transient elevations in cytoplasmic Ca2+. Under non-stimulated conditions, the same transport processes can regulate matrix Ca2+ concentrations and hence citric acid cycle activity.
Nicholls
David G
DG
Buck Institute for Age Research, Redwood Boulevard, Novato, CA 94945, USA. dnicholls@buckinstitute.org
D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D002130:Calcium Phosphates / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology*; D006801:Humans; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*
D002130:Calcium Phosphates; C020243:calcium phosphate