Immunization by Arg-gingipain A DNA vaccine protects mice against an invasive Porphyromonas gingivalis infection through regulation of interferon-gamma production.
We previously demonstrated that a Porphyromonas gingivalis rgpA DNA vaccine induced protective immune responses against P. gingivalis infection in mice. In the present study, reduction in lethality against infection by lethal doses of P. gingivalis was observed in the rgpA DNA vaccine-immunized mice. Cytokine levels in the mouse model with nonlethal doses of infection by P. gingivalis were evaluated to analyze the mechanism of protection by immunization with the rgpA DNA vaccine. After nonlethal challenge with invasive P. gingivalis W50, production of interleukin (IL)-2, IL-4, IL-5 and IL-12 was elevated; however, interferon (IFN)-gamma was lower in the serum of the DNA vaccine-immunized mice than in the serum of nonimmunized mice. The regulation of IFN-gamma production elicited by immunization with the rgpA DNA vaccine may play a significant role in protection against P. gingivalis infection in mice.
Yonezawa
H
H
Department of Microbiology, Tokyo Dental College, Chiba, Japan.
Kato
T
T
Kuramitsu
H K
HK
Okuda
K
K
Ishihara
K
K
D018829:Adhesins, Bacterial / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D016866:Bacteroidaceae Infections / Q000517:prevention & control*; D003546:Cysteine Endopeptidases / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins / Q000276:immunology*; D007114:Immunization; D007371:Interferon-gamma / Q000097:blood*; D018664:Interleukin-12 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D016966:Porphyromonas gingivalis / Q000145:classification / Q000235:genetics / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D007376:Interleukin-2; D015848:Interleukin-5; D019444:Vaccines, DNA; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma; D003546:Cysteine Endopeptidases