title: Three-dimensional micro-computed tomographic evaluation of periodontal regeneration: a human report of intrabony defects treated with Bio-Oss collagen.
abstract: This study utilized three-dimensional micro-computed tomography (micro-CT) to evaluate the regenerative response to Bio-Oss Collagen when used alone or in combination with a Bio-Gide bilayer collagen membrane for the treatment of four intrabony defects (5 to 7 mm) around single-rooted teeth. The micro-CT observations are compared to the clinical, radiographic, and histologic results, which have been previously reported. After reflecting a full-thickness flap, thorough degranulation and root planing were accomplished. Bio-Oss Collagen was then used to fill the defects, and in two cases a Bio-Gide membrane was placed over the filled defect. Radiographs, clinical probing depths, and attachment levels were obtained before treatment and immediately preceding en bloc resection of teeth and surrounding tissues 9 months later. A mean pocket depth reduction of 5.75 mm and mean clinical attachment level gain of 5.25 mm were recorded. The histologic evaluation demonstrated the formation of a complete new attachment apparatus with new cementum, periodontal ligament, and alveolar bone at the level of and coronal to the calculus reference notch. Micro-CT evaluation confirmed the histologic results and demonstrated the absence of ankylosis or root resorption for all specimens. This human histologic study demonstrated that Bio-Oss Collagen has the capacity to facilitate regeneration of the periodontal attachment apparatus when placed in intrabony defects. Micro-CT observations confirmed the histologic results and enhanced the three-dimensional understanding of periodontal wound healing. The results indicate that micro-CT may be useful for three-dimensional evaluation of periodontal regenerative procedures.
authors.affiliation: Department of Periodontology, Harvard School of Dental Medicine, Cambridge, Massachusetts, USA. marc_nevins@hms.harvard.edu
authors.forename: Marc L
authors.initials: ML
authors.lastname: Nevins
authors.forename: Marcelo
authors.initials: M
authors.lastname: Camelo
authors.forename: Alberto
authors.initials: A
authors.lastname: Rebaudi
authors.forename: Samuel E
authors.initials: SE
authors.lastname: Lynch
authors.forename: Myron
authors.initials: M
authors.lastname: Nevins
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use; D018786:Bone Substitutes / Q000627:therapeutic use*; D002417:Cattle; D003094:Collagen / Q000627:therapeutic use; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008903:Minerals / Q000627:therapeutic use*; D010865:Pilot Projects; D014057:Tomography, X-Ray Computed / Q000379:methods*
chemical_list: C106416:Bio-Gide; C077540:Bio-Oss; D001672:Biocompatible Materials; D018786:Bone Substitutes; D008903:Minerals; D003094:Collagen