West Nile virus detection in urine.
We report West Nile virus (WNV) RNA in urine collected from a patient with encephalitis 8 days after symptom onset. Viral RNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Sequence and phylogenetic analysis confirmed the PCR product to have > or = 99% similarity to the WNV strain NY 2000-crow3356.
Tonry
Jessica H
JH
Asia Pacific Institute of Tropical Medicine and Infectious Diseases John A. Burns School of Medicine, Honolulu, Hawaii 96816, USA. jtonry@rocketmail.com
Brown
Craig B
CB
Cropp
Cecil B
CB
Co
Juliene K G
JK
Bennett
Shannon N
SN
Nerurkar
Vivek R
VR
Kuberski
Timothy
T
Gubler
Duane J
DJ
D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D016000:Cluster Analysis; D006801:Humans; D008297:Male; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014901:West Nile Fever / Q000188:drug therapy / Q000652:urine*; D014902:West Nile virus / Q000235:genetics / Q000302:isolation & purification*
D000914:Antibodies, Viral; D012367:RNA, Viral