title: Circulating inflammatory endothelial cells contribute to endothelial progenitor cell dysfunction in patients with vasculitis and kidney involvement.
abstract: Impaired angiogenic function has been reported in patients with kidney failure. During vascular damage, endothelial cells may detach from the site of inflammation and be released into the peripheral blood. With the use of Wegener's granulomatosis as a study model, whether circulating inflammatory endothelial cells (IEC) can (1) be used as a disease activity marker and (2) contribute to sustained vascular damage by inducing endothelial progenitor cell (EPC) dysfunction were examined. IEC-defined as endothelial cells that express the two inflammatory-associated markers vascular-adhesion protein-1 (VAP-1) and MHC class I-related chain A (MICA)-were increased significantly in patients with active disease as compared with those in remission. IEC expressed high levels of inducible nitric oxide synthase and neutrophil-activating chemokines, such as macrophage inflammatory protein-1alpha, growth-related oncogene-alpha, epithelial neutrophil activating peptide-78, and IL-8, and induced increased neutrophil migration. IEC levels significantly correlated with C-reactive protein and extent of organ involvement. Patients with active disease had decreased numbers of EPC colony-forming units and a high expression of VAP-1 and MICA in kidney endothelium. EPC did not express VAP-1 or MICA. IEC significantly inhibited proliferation, migration, and endothelial nitric oxide synthase expression in EPC. Thus, apart from being a new disease activity marker, IEC may contribute to vascular damage by impairing the functional capacity for repair by EPC. IEC may provide a unique in vitro system to study pathogenesis of kidney and vascular diseases.
authors.affiliation: Department of Transplantation Surgery B56, Karolinska University Hospital at Huddinge, Stockholm S-141 86, Sweden.
authors.forename: Carolina
authors.initials: C
authors.lastname: Holmén
authors.forename: Elzafir
authors.initials: E
authors.lastname: Elsheikh
authors.forename: Peter
authors.initials: P
authors.lastname: Stenvinkel
authors.forename: Abdul Rashid
authors.initials: AR
authors.lastname: Qureshi
authors.forename: Erna
authors.initials: E
authors.lastname: Pettersson
authors.forename: Sirpa
authors.initials: S
authors.lastname: Jalkanen
authors.forename: Suchitra
authors.initials: S
authors.lastname: Sumitran-Holgersson
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006631:Amine Oxidase (Copper-Containing) / Q000096:biosynthesis; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D014890:Granulomatosis with Polyangiitis / Q000097:blood* / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D006801:Humans; D008297:Male; D008875:Middle Aged; D012795:Sialoglycoproteins / Q000096:biosynthesis; D013234:Stem Cells / Q000378:metabolism; D014657:Vasculitis / Q000097:blood* / Q000276:immunology*
chemical_list: D015815:Cell Adhesion Molecules; D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D012795:Sialoglycoproteins; C076668:AOC3 protein, human; D006631:Amine Oxidase (Copper-Containing)