Heterogeneity of axon terminals expressing VGLUT1 in the cerebral neocortex.
Using immunocytochemical techniques and confocal microscopy we have studied the localization of the vesicular glutamate transporters (VGLUTs) 1 and 2 in the mammalian cerebral cortex. The cardinal observations gathered to date can be summarized as follows: 1) Many VGLUT1-positive puncta coexpressing synaptophysin-1 outline pyramidal cell somata and proximal dendrites; of these, a sizeable fraction coexpress VGAT, the vesicular transporter for GABA; 2) VGLUT2-positive puncta are also present in layers II-III and some of them coexpress VGLUTI. These findings suggest that in the cerebral cortex of adult rats axon terminals expressing VGLUT1 are heterogeneous.
Conti
F
F
Department of Neuroscience, Section of Physiology, Università Politecnica delle Marche, Via Tronto 10/A, Torrette di Ancona, 1-60020 Ancona, Italy. f.conti@univpm.it
Candiracci
C
C
Fattorini
G
G
D026905:Amino Acid Transport Systems / Q000378:metabolism; D000818:Animals; D018698:Glutamic Acid / Q000378:metabolism*; D006801:Humans; D026901:Membrane Transport Proteins / Q000378:metabolism*; D019579:Neocortex / Q000378:metabolism* / Q000648:ultrastructure; D017729:Presynaptic Terminals / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology*; D050598:Vesicular Glutamate Transport Protein 1; D050599:Vesicular Glutamate Transport Protein 2; D050495:Vesicular Inhibitory Amino Acid Transport Proteins; D033921:Vesicular Transport Proteins / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D026905:Amino Acid Transport Systems; D026901:Membrane Transport Proteins; C493476:SLC17A6 protein, human; C493464:SLC17A7 protein, human; C118623:Slc32a1 protein, rat; D050598:Vesicular Glutamate Transport Protein 1; D050599:Vesicular Glutamate Transport Protein 2; D050495:Vesicular Inhibitory Amino Acid Transport Proteins; D033921:Vesicular Transport Proteins; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid