title: Multilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa.
abstract: A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, distributed across the X. fastidiosa genome, clearly differentiated and clustered X. fastidiosa strains collected from grape, almond, citrus, and oleander. They are well suited for differentiating strains and studying X. fastidiosa epidemiology and population genetics.
authors.affiliation: Crop Diseases, Pests & Genetics, U.S. Department of Agriculture-ARS, 9611 S. Riverbend Avenue, Parlier, CA 93648, USA. hlin@fresno.ars.usda.gov
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.forename: Edwin L
authors.initials: EL
authors.lastname: Civerolo
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.forename: Samuel
authors.initials: S
authors.lastname: Barros
authors.forename: Marta
authors.initials: M
authors.lastname: Francis
authors.forename: M Andrew
authors.initials: MA
authors.lastname: Walker
mesh_terms: D001483:Base Sequence; D002957:Citrus / Q000382:microbiology; D017931:DNA Primers; D005819:Genetic Markers; D014644:Genetic Variation; D018598:Minisatellite Repeats / Q000235:genetics*; D030007:Nerium / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D027861:Prunus / Q000382:microbiology; D017422:Sequence Analysis, DNA; D027843:Vitis / Q000382:microbiology; D044167:Xylella / Q000145:classification* / Q000235:genetics
chemical_list: D017931:DNA Primers; D005819:Genetic Markers