[Propanidid and etomidate identification from the blood by thin-layer chromatography].
Propanidid and etomidate were extracted from blood with diethyl ether at pH ca. 8 (phosphate buffer), then separated and identified by thin-layer chromatography on silica gel, using diethyl ether-acetone (3:1, v/v) for propanidid or dioxane-acetic acid (47:3, v/v) for etomidate as the mobile phase. Both compounds can be revealed on dried chromatograms using sodium carbonate solution up to the amount 1 microg/cm3 of plasma, and it is sufficient sensivity for propanidid adhibited in therapeutic doses, whereas this limits the detection of etomidate for overdosed patients only.
Zawisza
P
P
Akademia Medyczna, ul. Okopowa 1, 20-022 Lublin.
Przyborowski
L
L
D018686:Anesthetics, Intravenous / Q000097:blood; D002855:Chromatography, Thin Layer; D005045:Etomidate / Q000097:blood*; D006801:Humans; D011410:Propanidid / Q000097:blood*
D018686:Anesthetics, Intravenous; D011410:Propanidid; D005045:Etomidate