title: Cytogenetic and immunological changes after dermal exposure to polycyclic aromatic hydrocarbons and UV radiation.
abstract: Goeckerman's therapy (GT), which combines exposure to coal tar (polycyclic aromatic hydrocarbons - PAHs) and UV radiation (UV) is often used as the first option for treatment of psoriasis. However, PAHs and UV represent mutagenic, carcinogenic and immunotoxic agents. Therefore GT can represent a health risk for the patients. The group under observation consisted of thirty patients undergoing GT. Before and after the treatment, blood samples were collected and chromosomal aberrations and selected immunological markers were determined. The relationships between chromosomal aberrations and immunological markers and the extent (duration) of exposure to GT were evaluated. The Psoriasis Area and Severity Index (PASI) score confirmed the high efficacy of GT. However, significantly elevated levels of chromosomal aberrations of peripheral lymphocytes were also found after the therapy (p<0.001). The levels of chromosomal abnormalities correlated to the extent and the total duration of exposure to PAHs (r = 0.682, p<0.01 and r = 0.605, p<0.05). After the therapy, significantly decreased levels of IgE, IgM isotypes of immunoglobulin, alpha(2)-macroglobulin and transferrin together with beta(2)-microglobulin were found. From the immunological markers listed above only the decreased level of alpha(2)-macroglobulin correlated to the extent of exposure to PAHs (r = -0.568, p<0.05). No correlation was found between chromosomal aberrations, significantly changed immunological markers and the duration of UV exposure. Our study revealed that GT has a significant impact on both genetic and immunological parameters of psoriatic patients. The results indicate that GT could increase genotoxic risk and modulates immunity of treated patients.
authors.affiliation: Institute of Pathological Physiology, Charles University in Prague, Faculty of Medicine in Hradec Králové, Simkova 870, 500 38, Hradec Králové, Czech Republic. borka@lfhk.cuni.cz
authors.forename: L
authors.initials: L
authors.lastname: Borská
authors.forename: Z
authors.initials: Z
authors.lastname: Fiala
authors.forename: J
authors.initials: J
authors.lastname: Krejsek
authors.forename: K
authors.initials: K
authors.lastname: Hamáková
authors.forename: C
authors.initials: C
authors.lastname: Andrýs
authors.forename: J
authors.initials: J
authors.lastname: Smejkalová
authors.forename: D
authors.initials: D
authors.lastname: Vokurková
authors.forename: J
authors.initials: J
authors.lastname: Kremlácek
mesh_terms: D000279:Administration, Cutaneous; D000328:Adult; D002869:Chromosome Aberrations; D003033:Coal Tar / Q000009:adverse effects / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007107:Immune System / Q000187:drug effects* / Q000528:radiation effects*; D007073:Immunoglobulin E / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D011084:Polycyclic Aromatic Hydrocarbons / Q000008:administration & dosage / Q000009:adverse effects* / Q000627:therapeutic use; D011565:Psoriasis / Q000097:blood / Q000276:immunology / Q000628:therapy*; D012720:Severity of Illness Index; D012867:Skin / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D014168:Transferrin / Q000032:analysis; D016896:Treatment Outcome; D014467:Ultraviolet Therapy / Q000009:adverse effects*; D000511:alpha-Macroglobulins / Q000032:analysis; D001613:beta 2-Microglobulin / Q000097:blood
chemical_list: D007075:Immunoglobulin M; D011084:Polycyclic Aromatic Hydrocarbons; D014168:Transferrin; D000511:alpha-Macroglobulins; D001613:beta 2-Microglobulin; D007073:Immunoglobulin E; D003033:Coal Tar