TaqMan genotyping of insertion/deletion polymorphisms.
The 5' fluorogenic (TaqMan) assay has been successfully used in screening for single-nucleotide polymorphisms; the very few steps required and the ability to automate each step allow for high-throughput screening. Insertion/deletion polymorphisms are an important class of markers that can be studied for different applications, such as diagnostics, genome variation, and species identification. Polymerase chain reaction (PCR) and post-PCR analysis are required to score the insertion or the deletion allele. In this chapter, we describe an expansion of the TaqMan technology for a rapid, high-throughput, screening for insertion/deletion polymorphisms in which the exact endpoints are known. The method requires minimal post-PCR analysis and can be applied to polymorphisms of any size.
Robledo
Renato
R
Department of Biology, University of Cagliari, Italy.
Beggs
William R
WR
Bender
Patrick K
PK
D000483:Alleles; D017931:DNA Primers; D015342:DNA Probes; D005838:Genotype; D006801:Humans; D016254:Mutagenesis, Insertional; D020641:Polymorphism, Single Nucleotide; D017384:Sequence Deletion; D019914:Taq Polymerase / Q000235:genetics*
D017931:DNA Primers; D015342:DNA Probes; D019914:Taq Polymerase