Spectrofluorimetric assay of azathioprine and 6-mercaptopurine in human blood plasma.
In this review is discussed a new modified assay measuring Azathioprine (AZA) and 6-Mercaptopurine (6-MP) in human plasma using spectrofluorimetric method. After completed hydrolysis of AZA to 6-MP, the last has been oxidised to Purine-6-sulphonate by two methods: using potassium chromate in the first method and potassium permanganate in the second one. Fluorescence of the solutions was measured at 393 nm with an excitation wavelength of 289 nm. Percentage of 6-MP remaining in toluene in the first method was 58 to 64. Comparing both methods of determination 6-MP it was noticed, that the first method is repeatable and can be applied for therapeutic drug monitoring.
Gajewska
M
M
Department of Drug Chemistry, Institute of Drug Science, School of Medicine, 1 Banacha Str., 02-097 Warsaw, Poland.
Dzierzgowska
A
A
Pawiński
T
T
Czerwińska
K
K
D001379:Azathioprine / Q000097:blood*; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood; D015122:Mercaptopurine / Q000097:blood*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D007166:Immunosuppressive Agents; D015122:Mercaptopurine; D001379:Azathioprine