title: [Suppression of enhanced green fluorescent protein expression in cells by RNA interference].
abstract: OBJECTIVE
  To evaluate the role of RNA interference (RNAi) in silencing the enhanced green fluorescent protein (eGFP) expression in 293T and Mel cells.
  
  
  METHODS
  Nested-PCR was used to amplify H1 promoter from human 293T cells for driving RNAi synthesis. RNAi vectors (TR1) for silencing the eGFP expression was constructed. The eGFP vector and RNAi vector (TR1) were then co-transfected into the 293T and Mel cells, in which the silencing effect on eGFP expression was investigated by fluorescence microscopy, reverse transcription-PCR(RT-PCR), fluorescence-assited cell sorting(FACS) analysis and real-time RT-PCR.
  
  
  RESULTS
  RNAi could effectively reduce more than 50 percent of eGFP expression in 293T cells as well as in Mel cells.
  
  
  CONCLUSIONS
  The RNAi vector constructed in this way paper can effectively inhibit eGFP expression in cells.
authors.affiliation: Institute of Medical Genetics, School of Medicine, Shanghai Jiaotong University, 200040 PR China.
authors.forename: Shu-yang
authors.initials: SY
authors.lastname: Xie
authors.forename: Jing-zhi
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Shu-zhen
authors.initials: SZ
authors.lastname: Huang
authors.forename: Zhao-rui
authors.initials: ZR
authors.lastname: Ren
authors.forename: Yi-tao
authors.initials: YT
authors.lastname: Zeng
mesh_terms: D002460:Cell Line; D005434:Flow Cytometry; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins