Effects of the type of dietary fat on acetylcholine-evoked amylase secretion and calcium mobilization in isolated rat pancreatic acinar cells.
Olive oil is a major component of the Mediterranean diet, and its role in human health is being actively debated. This study aimed to clarify the mechanism of pancreatic adaptation to dietary fat. For this purpose, we examined whether dietary-induced modification of pancreatic membranes affects acinar cell function in response to the secretagogue acetylcholine (ACh). Weaning male Wistar rats were assigned to one of two experimental groups and fed for 8 weeks with a commercial chow (C) or a semisynthetic diet containing virgin olive oil as dietary fat (OO). The fatty acid composition of pancreatic plasma membranes was determined by gas-liquid chromatography. For assessment of secretory function, viable acini were incubated with ACh and amylase of supernatant was further assayed with a substrate reagent. Changes in cytosolic Ca(2+) concentration in response to ACh were measured by fura-2 AM fluorimetry. Compared to C rats, pancreatic cell membranes of OO rats had a higher level of monounsaturated fatty acids and a lower level of both saturated and polyunsaturated fatty acids, thus, reflecting the type of dietary fat given. Net amylase secretion in response to ACh was greatly enhanced after OO feeding, although this was not paralleled by enhancement of ACh-evoked Ca(2+) peak increases. In conclusion, chronic intake of diets that differ in the fat type influences not only the fatty acid composition of rat pancreatic membranes but also the responsiveness of acinar cells to ACh. This mechanism may be, at least in part, responsible for the adaptation of the exocrine pancreas to the type of fat available.
Yago
María D
MD
Departamento de Fisiología, Instituto de Nutrición y Tecnología de Alimentos, Universidad de Granada, Spain. mdyago@ugr.es
Díaz
Ricardo J
RJ
Martínez
María A
MA
Audi
Nama'a
N
Naranjo
José A
JA
Martínez-Victoria
Emilio
E
Mañas
Mariano
M
D000109:Acetylcholine / Q000494:pharmacology*; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage*; D002149:Energy Intake; D005227:Fatty Acids / Q000032:analysis; D008297:Male; D000069463:Olive Oil; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D010938:Plant Oils / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar
D004041:Dietary Fats; D005227:Fatty Acids; D000069463:Olive Oil; D010938:Plant Oils; D000681:Amylases; D000109:Acetylcholine; D002118:Calcium