title: Excitation energy trapping in photosystem I complexes depleted in Lhca1 and Lhca4.
abstract: We report a time-resolved fluorescence spectroscopy characterization of photosystem I (PSI) particles prepared from Arabidopsis lines with knock-out mutations against the peripheral antenna proteins of Lhca1 or Lhca4. The first mutant retains Lhca2 and Lhca3 while the second retains one other light-harvesting protein of photosystem I (Lhca) protein, probably Lhca5. The results indicate that Lhca2/3 and Lhca1/4 each provides about equally effective energy transfer routes to the PSI core complex, and that Lhca5 provides a less effective energy transfer route. We suggest that the specific location of each Lhca protein within the PSI-LHCI supercomplex is more important than the presence of so-called red chlorophylls in the Lhca proteins.
authors.affiliation: Vrije Universiteit Amsterdam, Department of Physics and Astronomy, Biophysics, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands. janihal@nat.vu.nl
authors.forename: Janne A
authors.initials: JA
authors.lastname: Ihalainen
authors.forename: Frank
authors.initials: F
authors.lastname: Klimmek
authors.forename: Ulrika
authors.initials: U
authors.lastname: Ganeteg
authors.forename: Ivo H M
authors.initials: IH
authors.lastname: van Stokkum
authors.forename: Rienk
authors.initials: R
authors.lastname: van Grondelle
authors.forename: Stefan
authors.initials: S
authors.lastname: Jansson
authors.forename: Jan P
authors.initials: JP
authors.lastname: Dekker
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D061146:Chlorophyll Binding Proteins; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D045331:Photosystem I Protein Complex / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D029681:Arabidopsis Proteins; D061146:Chlorophyll Binding Proteins; C490278:LHCA1 protein, Arabidopsis; C490277:Lhca2 protein, Arabidopsis; C493777:Lhca5 protein, Arabidopsis; D045342:Light-Harvesting Protein Complexes; D045331:Photosystem I Protein Complex; D010940:Plant Proteins