title: A rapid, sensitive electron-capture gas chromatographic procedure for analysis of metabolites of N-methyl,N-propargylphenylethylamine, a potential neuroprotective agent.
abstract: BACKGROUND
  N-Methyl,N-propargyphenylethylamine (MPPE) is a novel analog of (-)-deprenyl, a drug prescribed for Parkinson's disease and shown to have neuroprotective and neurorescue properties in a wide variety of in vitro and in vivo models. MPPE is also neuroprotective, but has the advantage over (-)-deprenyl of not being metabolized to amphetamine or N-methylamphetamine.
  
  
  METHODS
  In this paper, extractive derivatization with pentafluorobenzenesulfonyl chloride (PFBSC) followed by electron-capture gas chromatography was utilized to study the metabolism of MPPE.
  
  
  RESULTS
  The procedure is rapid and reproducible, giving derivatives with excellent chromatographic properties. Using this procedure, it has now been shown that beta-phenylethylamine (PEA), N-methylphenylethylamine (N-methylPEA) and N-propargylphenylethylamine (N-propargylPEA) are formed from MPPE during incubation of this drug with human liver microsomes. Levels of all three metabolites were shown to increase with increasing time of incubation with the microsomes.
  
  
  CONCLUSIONS
  Extractive derivatization with PFBSC followed by electron-capture gas chromatography represents an efficient means of separating and quantitating the metabolites of MPPE, a novel neuroprotective agent.
authors.affiliation: Neurochemical Research Unit, Department of Psychiatry, 1E7.31 Mackenzie Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2R7. kar9@ualberta.ca
authors.forename: Kay
authors.initials: K
authors.lastname: Rittenbach
authors.forename: B Duff
authors.initials: BD
authors.lastname: Sloley
authors.forename: Lei
authors.initials: L
authors.lastname: Ling
authors.forename: Ronald T
authors.initials: RT
authors.lastname: Coutts
authors.forename: Jackie
authors.initials: J
authors.lastname: Shan
authors.forename: Glen B
authors.initials: GB
authors.lastname: Baker
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D008694:Methamphetamine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D018696:Neuroprotective Agents / Q000032:analysis* / Q000378:metabolism; D010627:Phenethylamines / Q000032:analysis / Q000378:metabolism; D012642:Selegiline / Q000031:analogs & derivatives* / Q000032:analysis* / Q000378:metabolism; D013450:Sulfones; D013997:Time Factors; D005464:Fluorobenzenes; D005466:Fluorocarbons
chemical_list: D008694:Methamphetamine; D018696:Neuroprotective Agents; D010627:Phenethylamines; D012642:Selegiline; D013450:Sulfones; C508639:N-Methyl,N-propargyphenylethylamine; C027141:N-methylphenethylamine; C029261:phenethylamine; C043853:pentafluorobenzenesulfonyl chloride; D005464:Fluorobenzenes; D005466:Fluorocarbons