[In vivo micronuclei and gene mutation induced by DEN in the lacZ transgenic mouse].
OBJECTIVE
To assess mutagenicity of N-nitrosodiethylamine (DEN) in vivo using the lacZ transgenic mouse (Muta Mouse).


METHODS
LacZ gene was recovered through in vitro packing. Mutant frequencies (MFs) in the lacZ gene of liver, lung and bladder were examined after intraperitoneal treatment with DEN (25mg/kg per week x 4). Simultaneously, the clastogenicity of DEN was evaluated by the peripheral blood micronucleus assay.


RESULTS
The mean frequency of micronucleated reticulocytes (MNRETs) did not increase by the treatment with DEN. The MFs increase above spontaneous levels were apparent in liver and lung, with respectively 6.13 - and 3.66 fold increase. The MF of lacZ of bladder was of the background level.


CONCLUSIONS
Liver and lung are target organ of genotoxicity in vivo induced by DEN with different sensitivity.
Zhan
Li
L
National Chengdu Center for Safety Evaluation of Drugs, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Li-Shi
LS
Wang
Li
L
Zhu
Ling
L
Zhang
Hao
H
Suzuki
Takamasa
T
Wu
Desheng
D
D000818:Animals; D004052:Diethylnitrosamine / Q000633:toxicity*; D007763:Lac Operon / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D015162:Micronucleus Tests; D009154:Mutation / Q000187:drug effects*
D004052:Diethylnitrosamine