Diversification of CDK11 transcripts during chicken testis development and regression.
CDK11 (cyclin-dependent kinase 11, formerly known as PITSLRE) is a serine/threonine kinase that associates with the cyclin L2 regulatory partner. CDK11 catalytic activity has been associated with apoptosis, transcription, and RNA processing. Here, we identify novel chicken testis CDK11 transcripts that differ in their 5'UTR, 3'UTR, splicing of the exon 6, and polyadenylation. We have also characterized the differential expression of CDK11 in somatic tissues, during testis development and upon testicular regression by diethylstilbestrol (DES) treatment. The heterogeneity of CDK11 transcripts presented in this study suggests new possibilities for post-transcriptional regulation.
Francone
Victor P
VP
Departament de Ciències Fisiològiques I, Laboratori de Genètica Molecular, Institut d'Investigacions Biomediques August Pi i Sunyer, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain. vfrancone@uchc.edu
Mezquita
Jovita
J
D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D015152:Blotting, Northern; D002645:Chickens / Q000235:genetics* / Q000254:growth & development; D018844:Cyclin-Dependent Kinases / Q000235:genetics* / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004054:Diethylstilbestrol; D015971:Gene Expression Regulation, Enzymologic; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013737:Testis / Q000254:growth & development*
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D004054:Diethylstilbestrol; C447612:CDK11a protein, human; D018844:Cyclin-Dependent Kinases