title: Protein kinase D regulates vesicular transport by phosphorylating and activating phosphatidylinositol-4 kinase IIIbeta at the Golgi complex.
abstract: Protein kinase D (PKD) regulates the fission of vesicles originating from the trans-Golgi network. We show that phosphatidylinositol 4-kinase IIIbeta (PI4KIIIbeta) - a key player in the structure and function of the Golgi complex - is a physiological substrate of PKD. Of the three PKD isoforms, only PKD1 and PKD2 phosphorylated PI4KIIIbeta at a motif that is highly conserved from yeast to humans. PKD-mediated phosphorylation stimulated lipid kinase activity of PI4KIIIbeta and enhanced vesicular stomatitis virus G-protein transport to the plasma membrane. The identification of PI4KIIIbeta as one of the PKD substrates should help to reveal the molecular events that enable transport-carrier formation.
authors.affiliation: Institute for Cell Biology and Immunology, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany. angelika.hausser@izi.uni-stuttgart.de
authors.forename: Angelika
authors.initials: A
authors.lastname: Hausser
authors.forename: Peter
authors.initials: P
authors.lastname: Storz
authors.forename: Susanne
authors.initials: S
authors.lastname: Märtens
authors.forename: Gisela
authors.initials: G
authors.lastname: Link
authors.forename: Alex
authors.initials: A
authors.lastname: Toker
authors.forename: Klaus
authors.initials: K
authors.lastname: Pfizenmaier
mesh_terms: D019870:1-Phosphatidylinositol 4-Kinase / Q000378:metabolism*; D020816:Amino Acid Motifs / Q000502:physiology; D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D019556:COS Cells; D002462:Cell Membrane / Q000201:enzymology; D002522:Chlorocebus aethiops; D017124:Conserved Sequence / Q000502:physiology; D004789:Enzyme Activation / Q000502:physiology; D019143:Evolution, Molecular; D006056:Golgi Apparatus / Q000201:enzymology* / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D010766:Phosphorylation; D020033:Protein Isoforms / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D022161:Transport Vesicles / Q000201:enzymology*; D014759:Viral Envelope Proteins / Q000378:metabolism
chemical_list: C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D020033:Protein Isoforms; D014759:Viral Envelope Proteins; D019870:1-Phosphatidylinositol 4-Kinase; C089212:protein kinase D; D011493:Protein Kinase C