Two proline-rich peptides from pig (Sus scrofa) salivary glands generated by pre-secretory pathway underlying the action of a proteinase cleaving ProAla bonds.
The primary structures of two salivary proline-rich peptides (PRP-SP-A, M 6156.0 amu and PRP-SP-B, M 1905.0 amu), from pig (Sus scrofa) were determined. The PRP-SP-B peptide, 21 residues long, overlaps with a sequence repeated 43 times in three deposited cDNAs coding for PRP proteins cloned from porcine parotid glands (Swiss-Prot codes: Q95JC9, Q95JD1, Q95JD0). PRP-SP-A peptide, 56 amino acid residues long, overlaps with the N-terminus repeats of Q95JC9 and Q95JD1 and it is phosphorylated at Ser 12 and 14. The two peptides were found both in whole saliva and in granules from pig parotid glands. The biosynthesis of the two peptides implies the action of a proteinase responsible for Pro downward arrow Ala cleavage in the pre-secretory process.
Patamia
M
M
Istituto di Biochimica e Biochimica Clinica, Facoltà di Medicina, Università Cattolica e/o Istituto per la Chimica del Riconoscimento Molecolare, C.N.R., Largo F. Vito 1, 00168 Roma, Italy.
Messana
I
I
Petruzzelli
R
R
Vitali
A
A
Inzitari
R
R
Cabras
T
T
Fanali
C
C
Scarano
E
E
Contucci
A
A
Galtieri
A
A
Castagnola
M
M
D000409:Alanine / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002217:Carbachol / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary / Q000235:genetics; D030561:Databases, Nucleic Acid; D010450:Endopeptidases / Q000378:metabolism*; D005260:Female; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D010768:Phosphoserine / Q000032:analysis; D010862:Pilocarpine / Q000494:pharmacology; D011392:Proline / Q000378:metabolism; D055232:Proline-Rich Protein Domains; D012463:Saliva / Q000737:chemistry*; D012469:Salivary Glands / Q000187:drug effects / Q000378:metabolism*; D022142:Secretory Vesicles / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D021241:Spectrometry, Mass, Electrospray Ionization; D034421:Sus scrofa
D018076:DNA, Complementary; D010446:Peptide Fragments; D010455:Peptides; D010862:Pilocarpine; D010768:Phosphoserine; D002217:Carbachol; D011392:Proline; D010450:Endopeptidases; D000409:Alanine