Comparative genomic hybridization in extramammary Paget's disease.
BACKGROUND
Extramammary Paget's disease (EMPD) is a distinct skin cancer of unknown histogenesis. Data from genome-wide surveys for chromosomal aberrations in EMPD are limited.


OBJECTIVE
To identify chromosomal aberrations that are present in EMPD.


METHODS
Fifteen cases of EMPD were analysed by comparative genomic hybridization (CGH). We used pooled DNA CGH, instead of studying a single sample. In addition, immunohistochemistry was performed for detection of androgen receptor (AR).


RESULTS
The most recurrent change was amplification at chromosomes Xcent-q21 and 19, and loss at 10q24-qter. In addition, expression of AR, located in chromosome X, was found in six cases.


CONCLUSIONS
Results suggest that AR may play a role in EMPD tumorigenesis.
Lee
M-W
MW
Pathology, Asan Medical Center, College of Medicine, University of Ulsan, 388-1 Poongnap-Dong, Songpa-Gu, Seoul 138-736, Korea. miumiu@www.amc.seoul.kr
Jee
K-J
KJ
Gong
G-Y
GY
Choi
J-H
JH
Moon
K-C
KC
Koh
J-K
JK
D002869:Chromosome Aberrations; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D041321:Chromosomes, Human, X / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods; D010145:Paget Disease, Extramammary / Q000235:genetics*
D004273:DNA, Neoplasm