title: Generation of embryoid bodies from mouse embryonic stem cells cultured on STO feeder cells.
abstract: Embryoid bodies, which are similar to post-implantation egg-cylinder stage embryos, provide a model for the study of embryo development and stem cell differentiation. We describe here a novel method for generating embryoid bodies from murine embryonic stem (ES) cells cultured on the STO feeder layer. The ES cells grew into compact aggregates in the first 3 days of coculture, then became simple embryoid bodies (EBs) possessing primitive endoderm on the outer layer. They finally turned into cystic embryoid bodies after being transferred to Petri dishes for 1-3 days. Evaluation of the EBs in terms of morphology and differentiating potential indicates that they were typical in structure and could generate cells derived from the three germ layers. The results show that embryoid bodies can form not only in suspension culture but also directly from ES cells cultured on the STO feeder layer.
authors.affiliation: College of Life Science, Zhejiang University, Hangzhou, Zhejiang 310012, PR China.
authors.forename: Qing-Jun
authors.initials: QJ
authors.lastname: Zhou
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Shao
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Xiang
authors.forename: Ruo-Zhen
authors.initials: RZ
authors.lastname: Hu
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Lu
authors.forename: Hang
authors.initials: H
authors.lastname: Yao
authors.forename: Li-Cheng
authors.initials: LC
authors.lastname: Dai
mesh_terms: D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D002449:Cell Aggregation; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D019070:Cell Lineage; D018920:Coculture Techniques / Q000379:methods*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D050991:GATA4 Transcription Factor / Q000032:analysis / Q000235:genetics; D005855:Germ Layers / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D022781:Hepatocytes / Q000737:chemistry / Q000166:cytology; D051379:Mice; D046529:Microscopy, Electron, Transmission; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000166:cytology; D016900:Neurofilament Proteins / Q000032:analysis / Q000235:genetics; D009474:Neurons / Q000737:chemistry / Q000166:cytology; D012333:RNA, Messenger / Q000032:analysis; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D014212:Tretinoin / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000032:analysis / Q000235:genetics
chemical_list: D002087:Butyrates; D050991:GATA4 Transcription Factor; C484171:Gata4 protein, mouse; D016900:Neurofilament Proteins; D012333:RNA, Messenger; D000509:alpha-Fetoproteins; D014212:Tretinoin; D004121:Dimethyl Sulfoxide