[Effects of 5F from Pteri semipinnata on the expression of ETS-1 mRNA and VEGF protein of highly metastatic ovarian carcinoma HO-8910PM cells].
OBJECTIVE
To study the effect of 5F from Pteri semipinnata L. on the expression of ETS-1 mRNA and VEGF protein, and to investigate the antitumor mechanisms of 5F from Pteri semipinnata L.


METHODS
RT-PCR assay was used to assess the expression level of ETS-1 mRNA. The expression level of VEGF protein was assessed by Western blot analysis.


RESULTS
The expression level of of VEGF protein obviously decreased in HO-8910PM cells treated with 25 to approximately 100 micromol/L 5F for 24 hours. 5F significantly down-regulated the expression of ETS-1 mRNA.


CONCLUSIONS
The antitumor activity of 5F from Pteri semipinnata L is involved in the expression of ETS-1 mRNA and VEGF protein.
He
Taiping
T
Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023.
Mo
Lier
L
Liang
Nianci
N
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D004224:Diterpenes / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D050784:Proto-Oncogene Protein c-ets-1 / Q000096:biosynthesis* / Q000235:genetics; D032495:Pteris / Q000737:chemistry*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D050784:Proto-Oncogene Protein c-ets-1; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A