title: A genome scan for quantitative trait loci affecting the Salmonella carrier-state in the chicken.
abstract: Selection for increased resistance to Salmonella colonisation and excretion could reduce the risk of foodborne Salmonella infection. In order to identify potential loci affecting resistance, differences in resistance were identified between the N and 6(1) inbred lines and two QTL research performed. In an F2 cross, the animals were inoculated at one week of age with Salmonella enteritidis and cloacal swabs were carried out 4 and 5 wk post inoculation (thereafter called CSW4F2 and CSW4F2) and caecal contamination (CAECF2) was assessed 1 week later. The animals from the (N x 6(1)) x N backcross were inoculated at six weeks of age with Salmonella typhimurium and cloacal swabs were studied from wk 1 to 4 (thereafter called CSW1BC to CSW4BC). A total of 33 F2 and 46 backcross progeny were selectively genotyped for 103 and 135 microsatellite markers respectively. The analysis used least-squares-based and non-parametric interval mapping. Two genome-wise significant QTL were observed on Chromosome 1 for CSW2BC and on Chromosome 2 for CSW4F2, and four suggestive QTL for CSW5F2 on Chromosome 2, for CSW5F2 and CSW2BC on chromosome 5 and for CAECF2 on chromosome 16. These results suggest new regions of interest and the putative role of SAL1.
authors.affiliation: Unité de Génétique, Faculté d'ingénierie biologique, agronomique et environnementale, Université catholique de Louvain, Croix du Sud 2 bte 14, B-1348 Louvain-la-Neuve, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Tilquin
authors.forename: Paul A
authors.initials: PA
authors.lastname: Barrow
authors.forename: José
authors.initials: J
authors.lastname: Marly
authors.forename: Frédérique
authors.initials: F
authors.lastname: Pitel
authors.forename: Florence
authors.initials: F
authors.lastname: Plisson-Petit
authors.forename: Philippe
authors.initials: P
authors.lastname: Velge
authors.forename: Alain
authors.initials: A
authors.lastname: Vignal
authors.forename: Philippe V
authors.initials: PV
authors.lastname: Baret
authors.forename: Nat
authors.initials: N
authors.lastname: Bumstead
authors.forename: Catherine
authors.initials: C
authors.lastname: Beaumont
mesh_terms: D000818:Animals; D002353:Carrier State; D002645:Chickens / Q000235:genetics / Q000382:microbiology*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D016678:Genome; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats; D011201:Poultry Diseases / Q000235:genetics* / Q000382:microbiology; D040641:Quantitative Trait Loci; D012475:Salmonella / Q000235:genetics*; D012481:Salmonella Infections, Animal / Q000235:genetics* / Q000382:microbiology