title: Mast cell activation by lipoproteins.
abstract: Mast cells are activated by a number of agents that act independently from immunoglobulin E (IgE)-mediated hypersensitivity. One of these agents is oxidized low-density lipoprotein (oxLDL). OxLDL has been implicated in the pathogenesis of atherosclerosis and has been shown to induce microvascular dysfunction by the activation of mast cells. In this chapter, we describe the method for isolation of human LDL, oxidation of LDL, and demonstrate that oxLDL activates mast cells by measuring messenger ribonucleic acid (mRNA) levels and protein levels of interleukin (IL)-8 an inflammatory cytokine. IL-8 is a potent chemoattractant for neutrophils and monocytes, which would result in a chronic inflammatory response. IL-8 mRNA levels were measured by reverse-transcription polymerase chain reaction and protein levels by enzyme-linked immunoassay.
authors.affiliation: Department of Internal Medicine, Quillen College of Medicine, East Tennessee State University, Johnson City, TN, USA.
authors.forename: Jim
authors.initials: J
authors.lastname: Kelley
authors.forename: Gregory
authors.initials: G
authors.lastname: Hemontolor
authors.forename: Walid
authors.initials: W
authors.lastname: Younis
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Li
authors.forename: Guha
authors.initials: G
authors.lastname: Krishnaswamy
authors.forename: David S
authors.initials: DS
authors.lastname: Chi
mesh_terms: D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000276:immunology*; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D010084:Oxidation-Reduction
chemical_list: D003470:Culture Media; D016209:Interleukin-8; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D007073:Immunoglobulin E