Short-term exposures of fish to perfluorooctane sulfonate: acute effects on fatty acyl-coa oxidase activity, oxidative stress, and circulating sex steroids.
This study investigated the effects of exposure to waterborne perfluorooctane sulfonate (PFOS) on oxidative stress and reproductive endpoints in fish. Exposures utilized species commonly used in toxicological testing, including the fathead minnow (Pimephales promelas) and rainbow trout (Oncorhynchus mykiss), as well as relatively insensitive taxa such as creek chub (Semotilus atromaculatus), spottail shiner (Notropis hudsonius), and white sucker (Catostomus commersoni). In all fish species, short-term (14-28 d) exposure to PFOS produced only modest mortality at concentrations consistent with environmental spill scenarios. However, PFOS consistently increased hepatic fatty acyl-CoA oxidase activity and increased oxidative damage, as quantified using the 2-thiobarbituric acid-reactive substances assay. Plasma testosterone, 11-ketotestosterone, and 17beta-estradiol titers were often elevated with PFOS exposure. Vitellogenin, the egg yolk precursor protein, was occasionally altered in the plasma with PFOS exposure, but responses varied with maturity. Oviposition frequency and egg deposition in fathead minnow were not significantly impaired with PFOS exposure, despite a trend toward progressive impairment with increasing exposure concentrations. Although short-term PFOS exposure produced significant impacts on biochemical and reproductive endpoints in fish at concentrations consistent with environmental spills, the impact of long-term exposure to environmentally relevant concentrations of PFOS is unclear.
Oakes
Ken D
KD
Department of Zoology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Sibley
Paul K
PK
Martin
Jon W
JW
MacLean
Dan D
DD
Solomon
Keith R
KR
Mabury
Scott A
SA
Van Der Kraak
Glen J
GJ
D042962:Acyl-CoA Oxidase / Q000378:metabolism*; D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D004781:Environmental Exposure; D004958:Estradiol / Q000097:blood; D005260:Female; D005399:Fishes; D005466:Fluorocarbons / Q000633:toxicity*; D010058:Oviposition / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D013256:Steroids / Q000097:blood*; D013739:Testosterone / Q000031:analogs & derivatives / Q000097:blood; D014874:Water Pollutants, Chemical / Q000633:toxicity
D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D013256:Steroids; D014874:Water Pollutants, Chemical; D013739:Testosterone; D004958:Estradiol; C076994:perfluorooctane sulfonic acid; D042962:Acyl-CoA Oxidase; C003600:11-ketotestosterone