title: Localization of neuronal calcium sensor-1 at the adult and developing rat neuromuscular junction.
abstract: Neuronal calcium sensor (NCS-1; frequenin) is a calcium-binding protein involved in the regulation of neurotransmission in the central and peripheral nervous systems from insects to vertebrates. This study reports the localization of NCS-1 immunoreactivity, by Western blotting and immunohistochemistry, at the adult and developing postnatal rat neuromuscular junction. Our confocal immunofluorescence results on the whole-mount muscle and on semithin cross-sections are indicative of the localization of NCS-1 to motor axon terminals. There is no evidence of immunoreactivity in the postsynaptic side of the neuromuscular junctions or teloglial Schwann cells. These results suggest that NCS-1 is involved in the formation and function of presynaptic nerve terminal part of the neuromuscular junction during synaptogenesis and in adult mammals.
authors.affiliation: Unitat d'Histologia i Neurobiologia (UHN), Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: Neus
authors.initials: N
authors.lastname: Garcia
authors.forename: Maria A
authors.initials: MA
authors.lastname: Lanuza
authors.forename: Nuria
authors.initials: N
authors.lastname: Besalduch
authors.forename: Manel M
authors.initials: MM
authors.lastname: Santafe
authors.forename: Andreas
authors.initials: A
authors.lastname: Jeromin
authors.forename: Josep
authors.initials: J
authors.lastname: Tomas
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western / Q000379:methods; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods; D009121:Muscle Denervation / Q000379:methods; D009469:Neuromuscular Junction / Q000254:growth & development / Q000378:metabolism*; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism; D050765:Qa-SNARE Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011978:Receptors, Nicotinic / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; D050765:Qa-SNARE Proteins; D011978:Receptors, Nicotinic; D009418:S100 Proteins; C101464:frequenin calcium sensor proteins