Regulation of mast cell development.
Human mast cells originated from multipotential hematopoietic progenitors in bone marrow (BM). A large proportion of these progenitors were CD34+CD38-c-kit+ cells and CD34+CD38+c-kit+ cells. Cloning of stem cell factor (SCF) contributed to the development of culture systems of human mast cells from different sources including BM mononuclear cells, CD34+ cord blood (CB) cells, and fetal liver cells. SCF could support mast cell development from CD34+ CB cells in the serum-deprived culture system. The cumulative mast cell number reached 10(10)-fold the input quantity at 50 weeks of SCF-containing serumdeprived culture. CB-derived mast cells expressed tryptase, chymase, and histamine similar to mast cells residing in tissues. The surface expression of FcepsilonRI, however, was very low on fetal liver- or CB-derived mast cells. Factors that had effects on SCF-dependent human mast cell development were divided into four types: (1) factors that stimulated both proliferation and maturation: SCF; (2) factors that stimulated only proliferation: thrombopoietin, interleukin (IL)-9; (3) factors that inhibited proliferation, but stimulated maturation: IL-4, IL-6, and (4) factors that inhibited proliferation and maturation: retinoids.
Shiohara
Masaaki
M
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
Koike
Kenichi
K
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D006801:Humans; D007378:Interleukins / Q000494:pharmacology; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D012176:Retinoids / Q000494:pharmacology; D019089:Stem Cell Factor / Q000494:pharmacology; D013926:Thrombopoietin / Q000494:pharmacology
D003470:Culture Media; D007378:Interleukins; D012176:Retinoids; D019089:Stem Cell Factor; D013926:Thrombopoietin