title: Determination of alpha-hydroxy acids in cosmetic products by capillary electrophoresis.
abstract: In this work, a simple and reliable method for the simultaneous analysis of alpha-hydroxy acids such as tartaric, glycolic and lactic acids in cosmetic products was developed using capillary electrophoresis with indirect UV detection at 254 nm. A buffer solution containing 10 mmoll(-1) potassium phthalate (pH 4.1) and 0.5 mmoll(-1) cetyltrimethylammonium bromide as electroosmotic flow modifier allowed baseline resolution of the analytes in approximately 3 min. A few validation parameters of the proposed method include: good linearity for all compounds in the range from 10 to 100 mgl(-1) with coefficients of correlation larger than 0.9999. The average recoveries of tartaric, glycolic and lactic acids in commercial samples were 99.12, 99.41 and 99.43%, respectively. The method presented acceptable precision with average relative standard deviation of 0.54% (assay of commercial samples), 0.44% (peak area) and 0.16% (migration time).
authors.affiliation: Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, P.O. Box 66355, 05389-970 São Paulo, SP, Brazil.
authors.forename: Elizângela Abreu
authors.initials: EA
authors.lastname: Dutra
authors.forename: Maria Inês Rocha Miritello
authors.initials: MI
authors.lastname: Santoro
authors.forename: Gustavo Amadeu
authors.initials: GA
authors.lastname: Micke
authors.forename: Marina Franco Maggi
authors.initials: MF
authors.lastname: Tavares
authors.forename: Erika Rosa Maria
authors.initials: ER
authors.lastname: Kedor-Hackmann
mesh_terms: D003358:Cosmetics / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006016:Glycolates / Q000032:analysis; D006880:Hydroxy Acids / Q000032:analysis*; D019344:Lactic Acid / Q000032:analysis; D015203:Reproducibility of Results; D013644:Tartrates / Q000032:analysis
chemical_list: D003358:Cosmetics; D006016:Glycolates; D006880:Hydroxy Acids; D013644:Tartrates; C031149:glycolic acid; D019344:Lactic Acid; C029768:tartaric acid