Simultaneous choroidal tuberculoma and epididymo-orchitis caused by Mycobacterium tuberculosis.
OBJECTIVE
To report positive polymerase chain reaction (PCR) for Mycobacterium tuberculosis from the vitreous and epididymal fluid in a patient of choroidal tuberculoma and epididymitis.


METHODS
Interventional case report.


METHODS



METHODS
Institutional setting. intervention: Vitreous and epididymal fluid tap subjected to PCR for primer IS 6110 sequence specific for M. tuberculosis. Purification of DNA product and sequencing on an automated DNA sequencer. Institution of antitubercular therapy.


METHODS
Matching of sequence with NCBI's data bank response to antitubercular therapy.


RESULTS
DNA products from vitreous and epidiymal fluid matched with IS 6110 sequence of M. tuberculosis. Choroidal granuloma and epididymitis responded to antitubercular therapy.


CONCLUSIONS
Simultaneous choroidal tuberculoma and epididymitis resulted from an infection by M. tuberculosis, and both matched with IS 6110 sequence of M. tuberculosis.
Gupta
Vishali
V
Department of Ophthalmology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Gupta
Amod
A
Arora
Sunil
S
Sachdeva
Naresh
N
Bambery
Pardeep
P
D000328:Adult; D000995:Antitubercular Agents / Q000627:therapeutic use; D001826:Body Fluids / Q000382:microbiology; D015862:Choroid Diseases / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004823:Epididymitis / Q000382:microbiology*; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014375:Tuberculoma / Q000382:microbiology*; D014392:Tuberculosis, Ocular / Q000382:microbiology*; D014822:Vitreous Body / Q000382:microbiology
D000995:Antitubercular Agents; D004269:DNA, Bacterial