[Environmental distribution of Cryptococcus neoformans in the department of Cundinamarca-Colombia].
Cryptococcus neoformans is an opportunistic fungal pathogen that could cause infection in patients with immunodeficiency and healthy patients. The AIDS epidemic has shown the importance of studying the ecology and epidemiology of this fungus. The aim of this investigation was to determine if there was a relationship between the environmental distribution of the different varieties of C. neoformans and the climate zones in two transects located in department of Cundinamarca, in Colombia. For the isolation and identification of the yeast, conventional phenotypic methods were used and it was determined the population density (CFU/g of sample) and which was the variety of greater prevalence in each altitudinal rank. A total of 765 samples, from 26 municipalities were collected; of these 146 corresponded to pigeon droppings (Columba livia), 437 to Eucalyptus detritus (Eucalyptus camaldulensis and related species) and 182 to detritus of almond trees (Terminalia cattapa). C. neoformans was isolated from 46% of the studied municipalities, in both transects and the climate zones: warm, temperate and cold. The results indicated that the greater frequency of positive isolations came from the last climate zone (cold). The population density in pigeon excrements oscillated between 50 and 9.2 x 1,000,000, in eucalyptus between 500 and 10 x 1,000,000 and in almond trees was 50 CFU/g. Of 100,000 positive isolations 31% were serotype A, 59% serotype B and 10% serotype C; 96% of the isolates grew to 37 degrees C and all showed capsule. In conclusion, C. neoformans prevails in the three habitats studied but it showed a predilection for the cold thermal floor; the population densities did not allow defining a standard pattern of occurrence.
Quintero
Elizabeth
E
Universidad de Puerto Rico, Recinto Universitario de Mayaguez, Puerto Rico. elizaquinte@hotmail.com
Castañeda
Elizabeth
E
Ruiz
Alejandro
A
D000531:Altitude; D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology / Q000382:microbiology; D003105:Colombia / Q000453:epidemiology; D010856:Columbidae / Q000382:microbiology; D003453:Cryptococcosis / Q000453:epidemiology* / Q000382:microbiology / Q000662:veterinary; D003455:Cryptococcus neoformans / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D004197:Disease Reservoirs; D004783:Environmental Microbiology; D005052:Eucalyptus / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D027861:Prunus / Q000382:microbiology; D012703:Serotyping; D012988:Soil Microbiology; D013696:Temperature; D014329:Tropical Climate; D014504:Urban Health