[Co-expression of gpd1 and hor2 from Saccharomyces cerevisiae in Escherichia coli].
Based on the principle of the pathway engineering, a novel pathway of producing glycerol was built in E. coli. The gpd1 gene encoding glycerol 3-phosphate dehydrogenase and the hor2 gene encoding glycerol 3-phosphatase were cloned from Saccharomyces cerevisiae, respectively. The two genes were inserted into expression vector pSE380 together. A recombinant plasmid pSE-gpd1-hor2 containing polycistron was constructed under the control of the strong trc promoter. Then it was transformed into E. coli BL21. The result showed the recombinant microorganism GxB-gh could convert glucose to glycerol directly. And the recombinant microorganism GxB-gh was incubated to produce glycerol from D-glucose in the fermentor. The maximal concentration of glycerol was 46.67g/L at 26h. Conversion rate of glucose was 42.87%. The study is about "green" producing glycerol by recombinant microorganism and is also useful for further working in recombining microorganism of producing 1,3-propanediol.
Du
Li-Qin
LQ
Institute of Fermentation and Enzyme engineering, GuangXi University, Nanning 530005, China.
Wei
Yu-Tuo
YT
Chen
Fa-Zhong
FZ
Luo
Zhao-Fei
ZF
Huang
Ri-Bo
RB
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D005818:Genetic Engineering; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D005656:Fungal Proteins; D005993:Glycerolphosphate Dehydrogenase; D010744:Phosphoric Monoester Hydrolases; C040842:glycerol-1-phosphatase; D005990:Glycerol