Genetic analysis of two bile salt hydrolase activities in Lactobacillus acidophilus NCFM.
Two genes, bshA and bshB, encoding bile salt hydrolase enzymes (EC 3.5.1.24) were identified in the genome sequence of Lactobacillus acidophilus NCFM. Targeted inactivation of these genes via chromosomal insertion of an integration vector demonstrated different substrate specificities for these two enzymes.
McAuliffe
Olivia
O
Dept. of Food Science, North Carolina State University, Raleigh, NC 27695, USA.
Cano
Raul J
RJ
Klaenhammer
Todd R
TR
D000581:Amidohydrolases / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D001647:Bile Acids and Salts / Q000378:metabolism / Q000494:pharmacology*; D002876:Chromosomes, Bacterial; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D017353:Gene Deletion; D005822:Genetic Vectors; D006801:Humans; D007779:Lactobacillus acidophilus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013379:Substrate Specificity
D001426:Bacterial Proteins; D001647:Bile Acids and Salts; D003470:Culture Media; D004269:DNA, Bacterial; D000581:Amidohydrolases; C023154:choloylglycine hydrolase