title: Use of random amplified microsatellites to type isolates from an outbreak of nosocomial aspergillosis in a general medical ward.
abstract: Numerous patients were diagnosed with aspergillosis in a nosocomial outbreak caused by Aspergillus fumigatus and Aspergillus flavus. Thirty-three isolates of the former and 28 isolates of the latter were collected from the hospital environment and from the patients and studied for genetic relatedness by random amplified microsatellites (RAMS) analysis, in which two polymorphic regions were tested. Twenty-eight genotypes of A. fumigatus and 23 genotypes of A. flavus were identified. Four patients were infected by two isolates with the same genotype as the environmental isolates. One clinical genotype was shared by three patients and another was shared by two patients. We found that RAMS was useful for fingerprinting Aspergillus spp.
authors.affiliation: Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Guarro
authors.forename: María
authors.initials: M
authors.lastname: Solé
authors.forename: Roser
authors.initials: R
authors.lastname: Castany
authors.forename: Josep
authors.initials: J
authors.lastname: Cano
authors.forename: Anna
authors.initials: A
authors.lastname: Teixidó
authors.forename: Isabel
authors.initials: I
authors.lastname: Pujol
authors.forename: Josepa
authors.initials: J
authors.lastname: Gené
authors.forename: Antoni
authors.initials: A
authors.lastname: Castro
authors.forename: Pilar
authors.initials: P
authors.lastname: Sarda
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001228:Aspergillosis / Q000453:epidemiology* / Q000382:microbiology; D001231:Aspergillus flavus / Q000145:classification* / Q000235:genetics; D001232:Aspergillus fumigatus / Q000145:classification* / Q000235:genetics; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D017720:Molecular Epidemiology; D016533:Mycological Typing Techniques; D010362:Patients' Rooms; D019105:Random Amplified Polymorphic DNA Technique