[Development of methods on detection of enteropathogenic Escherichia coli by double PCR in meat and meat product].
OBJECTIVE
To establish a rapid method for detecting Enteropathogenic Escherichia coli (EPEC) in meat and meat product.


METHODS
Based on attaching and effacing lesion (eaeA gene) and bundle-forming pili (bfpA gene) of EPEC, two pairs of primers were designed. Then EPEC in meat and meat product were detected by double PCR.


RESULTS
The method is rapid and specific. The limit of detection is 10 cfu/g in 8 - 17 hours.


CONCLUSIONS
A simple, rapid, sensitive and specific PCR method can be established to detect EPEC in meat and meat product.
Shi
Yun
Y
Institute of Disease Control and Prevention, Acadimy of Military Medical Sciences, Beijing 100039, China.
Li
Ye-Peng
YP
Ji
Rong
R
D000818:Animals; D002417:Cattle; D054308:Enteropathogenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D008461:Meat Products / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine