[Fusion of TGF-beta 1 antigenic determinant and HBV core antigen as an anti-TGF-beta 1 vaccine].
OBJECTIVE
To examine the expression and purification of the TGFbeta1 vaccine from prokaryotic expression system and to determine the antigenicity of the fusion protein of recombinant vector pET28a/ HBcAg1-71-TGFbeta132-HBcAg89-144.


METHODS
The reconstructed vector pGEMEX-1/CTC was subcloned to pET28a and transformed into E. coli BL21 (DE3). The recombinant 6xHis- HBcAg1-71- TGFbeta132- HBcAg89-144 was to be expressed after induction by IPTG and purified with Ni-NTA-His affinity chromatography. The detection of the formation of core-like particles was done under an electron microscope and of their antigenity by using ELISA and Western blot.


RESULTS
A 2.46 x 10(4) protein was obtained by optimizing the conditions for both expression and purification. The protein had the TGFbeta1 antigenicity but not a HBc antigenity and the formed core-like particles were bigger than natural core particles.


CONCLUSIONS
The recombinant fusion protein in the prokaryotic expressed system can be used as an anti-TGFbeta1 vaccine to inhibit hepatic fibrosis.
Guo
Yong-hong
YH
Department of Infectious Diseases, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China. xiaoqing9759@hotmail.com
Luo
Jin-yan
JY
Hao
Zhi-ming
ZM
Wang
Quan-ying
QY
Yang
Guang-xiao
GX
D000939:Epitopes / Q000276:immunology*; D005822:Genetic Vectors; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008103:Liver Cirrhosis / Q000517:prevention & control*; D011387:Prokaryotic Cells / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000096:biosynthesis* / Q000276:immunology
D000939:Epitopes; D006512:Hepatitis B Core Antigens; D011993:Recombinant Fusion Proteins; D016212:Transforming Growth Factor beta; D014614:Vaccines, Synthetic