Biodegradation of pyridine in a completely mixed activated sludge process.
A potential bacterial culture (P1), isolated from garden soil and identified as Pseudomonas pseudoalcaligenes-KPN, was used as a starter seed to develop the biomass in a completely mixed activated sludge (CMAS) reactor and the system was evaluated for treatment of wastewater containing pyridine. The results of this study indicate that pyridine could be degraded efficiently at a loading of 0.251 kg pyridine kg MLSS(-1) d(-1) (0.156 kg TOC kg MLSS(-1) d(-1)) and at an optimal hydraulic retention time (HRT) of 24 h. Pyridine was used as the sole source of carbon and nitrogen by the biomass. Ammonia-nitrogen (NH3-N) was formed due to the metabolism of the pyridine ring. In the present investigation, the performance of CMAS with reference to pyridine biodegradation and the bio-kinetic constants for the biodegradation of pyridine, in a continuous system, were computed. The results indicate that a CMAS system inoculated with P. pseudoalcaligenes-KPN, under optimum conditions of HRT and pyridine loading, gives a yield coefficient of (Y) 0.29, decay coefficient (Kd) 0.0011 d(-1), maximum growth rate constant (mumax) 0.108 d(-1) and saturation rate constant (Ks) 5.37 mg L(-1) for pyridine.
Padoley
K V
KV
National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440 020, India.
Rajvaidya
A S
AS
Subbarao
T V
TV
Pandey
R A
RA
D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D007700:Kinetics; D044205:Pseudomonas pseudoalcaligenes / Q000378:metabolism; D011725:Pyridines / Q000378:metabolism*; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology; D012988:Soil Microbiology; D014865:Waste Disposal, Fluid / Q000379:methods*
D011725:Pyridines; D012722:Sewage; C023666:pyridine