The influence of ultrasound on the mechanical properties of healing tendons in rabbits.
BACKGROUND
Therapeutic ultrasound is commonly used for treatment of injuries to tendons, ligaments and joint capsules. Opinions still differ, however, as to the beneficial effects of ultrasound treatment of the above-mentioned conditions.


METHODS
We studied the effect of various ultrasound intensities on the healing of tenotomized, sutured and immobilized Achilles tendons of adult rabbits. Different intensities of pulsating ultrasound (0, 50, 100, 200, 500, 750, 1000 and 2000 mW/cm2, frequency 3 MHz) were given over the healing tendons for 5 min daily, using a gel as the coupling agent between the ultrasound probe and the skin. Eleven days after the tenotomy, the healing tendons were analyzed in a materials testing machine.


RESULTS
The extensibility of the healing tendons was greater after sonication at an intensity of 2000 mW/cm2 than after 50 mW/cm2. We found no significant effect on the load at rupture and normalized load at rupture of the ultrasound-treated healing tendons compared with mock-sonicated healing tendons. A gradual decline was observed, however, in the stiffness and collagen content of the healing tendons with increasing intensity of the ultrasound treatment.


CONCLUSIONS
The pulsating ultrasound treatment applied did not improve the mechanical properties of healing Achilles tendons at day 11 after the operation. On the other hand, a slight decline in stiffness was observed with increasing intensity of ultrasound treatment.
Larsen
Allan
A
Department of Connective Tissue Biology, Institute of Anatomy, University of Aarhus, Aarhus, Denmark.
Kristensen
Gert
G
Thorlacius-Ussing
Ole
O
Oxlund
Hans
H
D000125:Achilles Tendon / Q000293:injuries / Q000502:physiology*; D000818:Animals; D001696:Biomechanical Phenomena; D005260:Female; D011817:Rabbits; D014464:Ultrasonic Therapy; D014945:Wound Healing / Q000502:physiology*