title: Analysis of expressed sequence tags of the water flea Daphnia magna.
abstract: To study gene expression in the water flea Daphnia magna we constructed a cDNA library and characterized the expressed sequence tags (ESTs) of 7210 clones. The EST sequences clustered into 2958 nonredundant groups. BLAST analyses of both protein and DNA databases showed that 1218 (41%) of the unique sequences shared significant similarities to known nucleotide or amino acid sequences, whereas the remaining 1740 (59%) showed no significant similarities to other genes. Clustering analysis revealed particularly high expression of genes related to ATP synthesis, structural proteins, and proteases. The cDNA clones and EST sequence information should be useful for future functional analysis of daphnid biology and investigation of the links between ecology and genomics.
authors.affiliation: Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Watanabe
authors.forename: Norihisa
authors.initials: N
authors.lastname: Tatarazako
authors.forename: Shigeto
authors.initials: S
authors.lastname: Oda
authors.forename: Hiroyo
authors.initials: H
authors.lastname: Nishide
authors.forename: Ikuo
authors.initials: I
authors.lastname: Uchiyama
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Morita
authors.forename: Taisen
authors.initials: T
authors.lastname: Iguchi
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D003621:Daphnia / Q000235:genetics*; D020224:Expressed Sequence Tags; D015870:Gene Expression; D015723:Gene Library; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary