title: Dendritic-NK cell interactions in P. gingivalis-specific responses.
abstract: Patients with localized aggressive periodontitis have type-1 cytokines in gingival crevicular fluid and high titers of IFN-gamma-dependent IgG2 reactive with P. gingivalis in gingival crevicular fluid and serum. Localized aggressive periodontitis monocytes spontaneously differentiate into dendritic cells that can stimulate IFN-gamma production by NK cells. These relationships prompted the hypothesis that P. gingivalis-dendritic cell-NK cell interactions might promote type-1 cytokine responses. Although P. gingivalis is not a potent inducer of Th1 responses, it stimulated strong IL-12 responses by monocyte-derived dendritic cells in the presence of IFN-gamma, and IFN-gamma was produced by NK cells within 24 hrs in the presence of dendritic cells. Anti-P. gingivalis IgG2 responses were enhanced by dendritic cells, and removal of NK cells reduced IFN-gamma- and P. gingivalis-specific IgG2. Thus, P. gingivalis-dendritic cell-NK cell interactions apparently resulted in reciprocal stimulation and increased type-1 cytokine production by both dendritic cells and NK cells, and increased P. gingivalis-specific IgG2.
authors.affiliation: Clinical Research Center for Periodontal Diseases, School of Dentistry, VCU, PO Box 980556, Richmond, VA 23298-0556, USA.
authors.forename: T
authors.initials: T
authors.lastname: Kikuchi
authors.forename: D L
authors.initials: DL
authors.lastname: Willis
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.forename: D B
authors.initials: DB
authors.lastname: Purkall
authors.forename: S
authors.initials: S
authors.lastname: Sukumar
authors.forename: S E
authors.initials: SE
authors.lastname: Barbour
authors.forename: H A
authors.initials: HA
authors.lastname: Schenkein
authors.forename: J G
authors.initials: JG
authors.lastname: Tew
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000378:metabolism; D002450:Cell Communication; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism / Q000382:microbiology / Q000502:physiology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000378:metabolism / Q000382:microbiology / Q000502:physiology*; D051379:Mice; D009504:Neutrophils / Q000378:metabolism; D010518:Periodontitis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma