Phenolic acids in neem (Azadirachta indica): a major pre-existing secondary metabolites.
High Performance Liquid Chromatographic (HPLC) analyses of various parts (fresh and dry bark of stem, mature and tender leaves, flower and different parts of fruit, i.e., raw and ripe fruit epicarp, mesocarp and seed) of neem (Azadirachta indica), which occupies an important place in socio-cultural-religious life in Indian communities, indicate that neem is rich in pre-existing secondary metabolites (phenolic acids). Dry bark showed only tannic acid but in fresh bark three phenolic acids were observed, i.e., gallic, tannic, and ferulic acids. In tender leaves only gallic and ferulic acids were detected, but the levels of these phenolic acids in mature leaves were about three times and fifty times greater, respectively. Flowers had only two phenolic acids in which gallic acid was maximum followed by chlorogenic acid. The level of phenolic acid was maximum in seeds followed by epicarp and pulp. In raw and ripe fruit seeds four phenolic acids were detected. Raw fruit seeds were rich in phenolic acids than ripe fruit seeds. Fruit epicarp was relatively richer than seed, seed pulp and flowers of the plants. Neem flowers were also rich in gallic and chlorogenic acids.
Singh
U P
UP
Department of Mycology and Plant Pathology, Banaras Hindu University, Varanasi 221005, India. ups@banaras.ernet.in
Maurya
S
S
Singh
D P
DP
D031601:Azadirachta; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000032:analysis; D005707:Gallic Acid / Q000032:analysis; D006801:Humans; D062385:Hydroxybenzoates / Q000032:analysis*; D007194:India; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D013634:Tannins / Q000032:analysis
D003373:Coumaric Acids; D062385:Hydroxybenzoates; D010936:Plant Extracts; D013634:Tannins; D005707:Gallic Acid; C004999:ferulic acid; C017616:phenolic acid