[Effects of methamidophos on microtubule and microfilament proteins in sciatic nerve of hens].
OBJECTIVE
To investigate the dynamic changes of alpha-tubulin, beta-tubulin and beta-actin in sciatic nerve of hen with organophosphorus ester-induced delayed neuropathy (OPIDN).


METHODS
OPIDN was induced in 10-month-old Roman hens by daily subcutaneous administration of 30 mg/kg methamidophos for 15 days. Hens were sacrificed 2, 10, and 23 days respectively after manifesting neuropathy. The sciatic nerves were dissected, homogenized and used for the determination of the alpha-tubulin, beta-tubulin and beta-actin levels by western blotting.


RESULTS
The levels of alpha-tubulin in supernatant of sciatic nerves were decreased by 6%, 15% and 25% respectively on day 2, 10 and 23 respectively, while those in pellet remained almost unchanged. beta-tubulin were decreased by 27%, 6%, 19% in pellet and 1%, 21%, 22% in supernatant of sciatic nerves on 2, 10 and 23 days. Beta-actin level in pellet of sciatic nerve increased by 24%, 48% and 17% on day 2, 10 and 23, and little changes were observed in supernatant.


CONCLUSIONS
Methamidophos may induced changes of alpha-tubulin, beta-tubulin and beta-actin levels in sciatic nerve of hen, which may be one of the mechanism of the contribution to the occurrence and development of OPIDN.
Zhao
Xiu-lan
XL
Institute of Toxicology, School of Public Health, Shandong University, Jinan, Shandong Province 250012, China.
Xie
Ke-qin
KQ
Han
Xiao-ying
XY
Yu
Li-hua
LH
Zhu
Zhen-ping
ZP
Zhang
Tian-liang
TL
Zhang
Cui-li
CL
D000199:Actins / Q000378:metabolism; D000818:Animals; D002645:Chickens; D005260:Female; D007306:Insecticides / Q000633:toxicity*; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D014404:Tubulin; C014655:methamidophos