title: Screening and purification of a novel trypsin inhibitor from Prosopis juliflora seeds with activity toward pest digestive enzymes.
abstract: Several pests are capable of decreasing crop production causing severe economical and social losses. Aiming to find novel molecules that could impede the digestion process of different pests, a screening of alpha-amylase and trypsin-like proteinase inhibitors was carried out in Prosopis juliflora, showing the presence of both in dry seeds. Furthermore, a novel trypsin inhibitor, with molecular mass of 13,292 Da, was purified showing remarkable in vitro activity against T. castaneum and C. maculatus.
authors.affiliation: Center for Plant Molecular Biology, Tamil Nadu and Agricultural University, Coimbatore-641003, India.
authors.forename: S
authors.initials: S
authors.lastname: Sivakumar
authors.forename: O L
authors.initials: OL
authors.lastname: Franco
authors.forename: P D
authors.initials: PD
authors.lastname: Tagliari
authors.forename: C
authors.initials: C
authors.lastname: Bloch
authors.forename: M
authors.initials: M
authors.lastname: Mohan
authors.forename: B
authors.initials: B
authors.lastname: Thayumanavan
mesh_terms: D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D029862:Prosopis / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D010940:Plant Proteins; D014361:Trypsin Inhibitors; D000516:alpha-Amylases; D014357:Trypsin