Chemiluminescence of iron-chlorophyllin.
The iron-chlorophyllin complex was found to be chemiluminescent (CL) in an acetonitrile (22%)/water mixed solvent. In the presence of hydrogen peroxide, when iron-chlorophyllin was added to the mixed solvent, a sharp CL signal immediately appeared. Also, analysis of the absorption spectra revealed decomposition of iron-chlorophyllin (based on decrease in absorbance at 396 nm), hence iron-chlorophyllin is the CL substance. Moreover, the CL intensity decreased in the presence of potassium thiocyanate (KSCN), indicating that the axial coordinative position of iron-chlorophyllin acts as a point of catalytic activation. In addition, when fluorophores were present with iron-chlorophyllin CL, their CL intensity values were similar to or greater than that of the well-known trichlorophenylperoxalate ester (TCPO) CL. Thus, during the decomposition reaction of iron-chlorophyllin, the latter transfers its energy to the coexisting fluorophores. Moreover, since the decomposed compound in this CL reaction had a fluorescence, it was found that the iron-chlorophyllin also functions as an energy donor. Therefore, the iron-chlorophyllin complex acts not only as a CL substance, but also as a catalyst and energy donor in the reaction.
Nagoshi
Toshimasa
T
Department of Materials Science, Faculty of Engineering, Ibaraki University, Nakanarusawa, Hitachi 316-8511, Japan.
Ohno
Osamu
O
Kotake
Tomohiko
T
Igarashi
Shukuro
S
D002384:Catalysis; D002735:Chlorophyllides / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D006454:Hemoglobins / Q000737:chemistry; D007501:Iron / Q000737:chemistry*; D049449:Luminescence; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity
D002735:Chlorophyllides; D006454:Hemoglobins; D009942:Organometallic Compounds; D007501:Iron; C007020:chlorophyllin