Accumulation of 23 kDa lipocalin during brain development and injury in Hyphantria cunea.
The cDNA corresponding to a novel lipocalin was identified from the fall webworm, Hyphantria cunea. This lipocalin cDNA encodes a 194 residue protein with a calculated molecular mass of 23 kDa. Sequence analyses revealed that the 23 kDa lipocalin cDNA is most similar to Drosophila lazarillo, human apolipoprotein D, and Bombyrin. Northern blot analyses showed that 23 kDa lipocalin transcript is expressed in the whole body only in 4- and 6-day-old pupae. By Western blot analysis it was confirmed that 23 kDa lipocalin is mainly accumulated in brain and subesophageal ganglion, though it is detected in a small amount in fat body and epidermis of Hyphantria cunea. The accumulation of 23 kDa lipocalin in brain tissue was upregulated in response to injury. The putative function of 23 kDa lipocalin in brain is discussed.
Kim
Hong Ja
HJ
Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Je
Hyun Jeong
HJ
Cheon
Hyang Mi
HM
Kong
Sun Young
SY
Han
JikHyun
J
Yun
Chi Young
CY
Han
Yeon Su
YS
Lee
In Hee
IH
Kang
Young Jin
YJ
Seo
Sook Jae
SJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D029721:Drosophila Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009036:Moths / Q000254:growth & development*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D011679:Pupa; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins