Surface plasmon resonance for real-time evaluation of immobilized fructosyltransferase activity.
The extracellular enzyme fructosyltransferase (FTF) is considered to be a significant virulence factor in the dental biofilm. We have developed a method using surface plasmon resonance to detect the activity of immobilized FTF in situ. This real time technique provides a sensitive direct assay for characterizing functional properties of immobilized enzymes such as FTF.
Shemesh
Moshe
M
Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah POB 12272, Jerusalem 91120, Israel.
Steinberg
Doron
D
D018441:Biofilms; D003773:Dental Plaque / Q000382:microbiology; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005630:Fructans / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D013295:Streptococcus mutans / Q000201:enzymology / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*; D001426:Bacterial Proteins
D004800:Enzymes, Immobilized; D005630:Fructans; D005632:Fructose; D005947:Glucose; D006602:Hexosyltransferases; C021451:inulosucrase; D001426:Bacterial Proteins