[Detecting the DNA damage of N-ras gene induced by potassium dichromate].
OBJECTIVE
Applying RDPCR to detect the DNA damage of N-ras gene induced by Potassium dichromate


METHODS
Preparing single-stranded probes of exon 1 of N-ras gene in human. The genomic DNA was treated with Potassium dichromate, then amplified by RDPCR and detected by Southern hybridization with the probe.


RESULTS
The clear hybridized bands can be seen in the position which is induced by Potassium dichromate on the dose of 100 micromol/L, but can't be detected on the dose over 1000 mol/L.


CONCLUSIONS
It indicates that Potassium dichromate can cause the DNA damage of N-ras gene, which should be the key point of its carcinogenesis mechanisms.
Rao
Chao-Long
CL
Department of Environmental Health, School of Public Health, Sichuan University, Chengdu 610041, China.
Heng
Zheng-Chang
ZC
D004249:DNA Damage; D004785:Environmental Pollutants / Q000633:toxicity*; D011905:Genes, ras; D006801:Humans; D011192:Potassium Dichromate / Q000633:toxicity*
D004785:Environmental Pollutants; D011192:Potassium Dichromate