Expression of heparanase mRNA in anti-sense oligonucleotide-transfected human esophageal cancer EC9706 cells.
OBJECTIVE
To investigate the effects of anti-sense oligonucleotides (ASODNs) on mRNA expression of heparanase in human esophageal cancer EC9706 cells.


METHODS
One non-sense oligonucleotide (N-ODN) and five ASODNs against different heparanase mRNA sites were transfected into EC9706 cells, then the expression of heparanase mRNA in EC9706 cells was studied by in situ hybridization.


RESULTS
The expression of heparanase mRNA could be inhibited by ASODNs. There was no significant difference among five ASODNs (P>0.05), but there was a significant difference between ASODNs and N-ODN or non-transfected group (ASODN1: 2.25+/-0.25, ASODN2: 2.21+/-0.23, ASODN3: 2.23+/-0.23, ASODN4: 2.25+/-0.24 vs N-ODN: 3.47+/-2.80 or non- transfected group: 3.51+/-2.93 respectively, P<0.05).


CONCLUSIONS
The expression of heparanase mRNA in EC9706 cells can be inhibited by ASODNs in vivo, and heparanase ASODNs can inhibit metastasis of esophageal squamous cell carcinoma or other tumors by inhibiting the expression of heparanase.
Chen
Kui-Sheng
KS
Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Key Laboratory of Henan Tumor Pathology, Zhengzhou 450052, Henan Province, China. chenksh2002@yahoo.com.cn
Zhang
Lan
L
Tang
Lin
L
Zhang
Yun-Han
YH
Gao
Dong-Ling
DL
Yan
Liang
L
Zhang
Lei
L
D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005966:Glucuronidase / Q000235:genetics*; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D014162:Transfection; D000099175:Heparanase
D005966:Glucuronidase; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D000099175:Heparanase