title: [Affection of different preparations on the content of toxic constituents in traditional Chinese medicines, examplified with Caulis Aristolochiae Manshuriensis].
abstract: OBJECTIVE
  Taking Caulis Aristolochiae Manshuriensis (Guanmutong in Chinese, derived from the stem of Aristolochia manshuriensis) as an example, to study the affection of different preparations on the content of toxic constituents in traditional Chinese medicines.
  
  
  METHODS
  The separation was performed on a zorbax SB-C18 column with mobile phase of acetonitrile-3.7 mmol x L(-1) phosphoric acid buffer, detected at 260 nm.
  
  
  RESULTS
  The extraction percentage of aristolochic acids I, II and IV a in water extraction (1 h x 2) of Guanmutong were 53.4%, 75.5% and 61.9%, respectively; the remaining quantity of aristolochic acids I, II and IVa in the dregs of the decoction were 22.3%, 15.7% and 30.3%, respectively; Aristolochic acid I was still main substance among these aristolohic acids in the decoction of Guanmutong.
  
  
  CONCLUSIONS
  The content of toxic constituents of the traditional Chinese medicines varies evidently with different preparations of Guanmutong. So the preparation methods of traditional Chinese medicines should be suitably selected according to characteristics of the toxic constituents so as to lessen the body damages of human.
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
authors.forename: Cui-Ying
authors.initials: CY
authors.lastname: Zhang
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Ming-Ying
authors.initials: MY
authors.lastname: Shang
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Jun-Yu
authors.initials: JY
authors.lastname: Xu
authors.forename: Zhen-Guo
authors.initials: ZG
authors.lastname: Lh
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Li
authors.forename: Shao-Qing
authors.initials: SQ
authors.lastname: Cai
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Namba
mesh_terms: D030022:Aristolochia / Q000737:chemistry*; D034341:Aristolochic Acids / Q000032:analysis*; D004350:Drug Residues / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry* / Q000302:isolation & purification; D019999:Pharmaceutical Solutions / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders / Q000737:chemistry
chemical_list: D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal; D019999:Pharmaceutical Solutions; D011208:Powders