A transposon-based activation-tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations.
A population of 9471 stable activation-tagged lines was generated by transposable element mediated activation tagging mutagenesis in Arabidopsis (TAMARA) using the maize En/Spm transposon system. Based on DNA gel blot and flanking sequence analysis, this population contains approximately 6000 independent transposon insertions. A greenhouse-based screen identified six dominant or semi-dominant activation tagged mutants with obvious developmental alterations, among these a new pistillata mutant allele. In addition, a subset of 1500 lines was screened by a HPLC based high-throughput method for dominant activation tagged mutants with enhanced contents of phenolic compounds. One dominant activation tagged mutant (hpc1-1D) was isolated showing accumulation of a particular compound due to the upregulation of an R2R3-MYB transcription factor.
Schneider
Anja
A
Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50931 Köln, Germany. Anja.Schneider@uni-koeln.de
Kirch
Thomas
T
Gigolashvili
Tamara
T
Mock
Hans-Peter
HP
Sonnewald
Uwe
U
Simon
Rüdiger
R
Flügge
Ulf-Ingo
UI
Werr
Wolfgang
W
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism; D032461:Chromosomes, Plant; D004252:DNA Mutational Analysis; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016415:Sequence Alignment; D016324:Sequence Tagged Sites
D004251:DNA Transposable Elements; D005819:Genetic Markers