[Evaluation of IGL-1, a new organ preservation solution: preclinical results in renal transplantation].
BACKGROUND
Renal ischaemia and reperfusion lesions partly determine short-term and long-term graft survival. Organ preservation conditions appear to play a decisive role. This article presents the preclinical experimental results obtained in renal transplantation with an extracellular organ preservation solution, in which polyethylene glycol (PEG) is used as colloid.


RESULTS
The effects of inversion of Na+ and K+ gradients in the IGL-1 preservation solution compared to UW and replacement of hydroxylethyl starch (HES) by PEG were evaluated in an ex vivo isolated, perfused rat kidney model and then in a pig renal autotransplantation model. In these experimental models, after 24 hours of static storage, the sodium reabsorption fraction correlated with the quality of tubular function of the kidney and the glomerular filtration rate were constantly better in the IGL-1 group than in the UW group. In vivo, in the pig, resumption of renal function was significantly better in the IGL-1 group and histological examination demonstrated a significant reduction of expression of Major Histocompatibility Complex (MHC) type II, an indirect marker of inflammation, but also a reduction of markers of apoptosis and fibrosis for kidneys preserved in IGL-1.


CONCLUSIONS
In animal renal transplantation, IGL-1 ensures better resumption of renal function than UW, which currently remains the "gold standard"preservation solution. Further studies must be conducted to determine whether this new generation solution can replace UW as the reference solution.
Badet
Lionel
L
Service d'Urologie et Transplantation, Hôpital Edouard Herriot, Lyon, France. lionel.badet@chu-lyon.fr
Abdennebi
Hassen Ben
HB
Petruzzo
Palmina
P
McGregor
Brigitte
B
Espa
Michele
M
Hadj-Aissa
Aoumeur
A
Ramella-Virieux
Silvina
S
Steghens
Jean-Paul
JP
Portoghese
Francesca
F
Morelon
Emmanuel
E
Martin
Xavier
X
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D005919:Glomerular Filtration Rate; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007668:Kidney; D016030:Kidney Transplantation; D008297:Male; D023421:Models, Animal; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011092:Polyethylene Glycols; D051381:Rats; D017207:Rats, Sprague-Dawley; D014182:Transplantation, Autologous
D015415:Biomarkers; D000949:Histocompatibility Antigens Class II; D019188:Organ Preservation Solutions; D011092:Polyethylene Glycols