Complete genome sequence and lytic phase transcription profile of a Coccolithovirus.
The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi. Among the 472 predicted genes of the 407,339-base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi.
Wilson
William H
WH
Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK. whw@pml.ac.uk
Schroeder
Declan C
DC
Allen
Michael J
MJ
Holden
Matthew T G
MT
Parkhill
Julian
J
Barrell
Bart G
BG
Churcher
Carol
C
Hamlin
Nancy
N
Mungall
Karen
K
Norbertczak
Halina
H
Quail
Michael A
MA
Price
Claire
C
Rabbinowitsch
Ester
E
Walker
Danielle
D
Craigon
Marie
M
Roy
Douglas
D
Ghazal
Peter
P
D017209:Apoptosis; D001482:Base Composition; D002518:Ceramides / Q000096:biosynthesis; D019295:Computational Biology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D056890:Eukaryota / Q000821:virology; D015870:Gene Expression; D020869:Gene Expression Profiling; D005814:Genes, Viral; D016679:Genome, Viral; D020411:Oligonucleotide Array Sequence Analysis; D010447:Peptide Hydrolases / Q000235:genetics; D017862:Phycodnaviridae / Q000145:classification / Q000235:genetics* / Q000502:physiology*; D011401:Promoter Regions, Genetic; D021122:Protein Subunits; D017422:Sequence Analysis, DNA; D013107:Sphingolipids / Q000096:biosynthesis; D014158:Transcription, Genetic; D014779:Virus Replication
D002518:Ceramides; D004279:DNA, Viral; D021122:Protein Subunits; D013107:Sphingolipids; D012321:DNA-Directed RNA Polymerases; D010447:Peptide Hydrolases