title: Fine mapping of porcine chromosome 6 QTL and LEPR effects on body composition in multiple generations of an Iberian by Landrace intercross.
abstract: The leptin receptor gene (LEPR) is a candidate for traits related to growth and body composition, and is located on SSC6 in a region where fatness and meat composition quantitative trait loci (QTL) have previously been detected in several F2 experimental designs. The aims of this work were: (i) to fine map these QTL on a larger sample of animals and generations (F3 and backcross) of an Iberian x Landrace intercross and (ii) to examine the effects of LEPR alleles on body composition traits. Eleven single nucleotide polymorphisms (SNPs) were detected by sequencing LEPR coding regions in Iberian and Landrace pig samples. Three missense polymorphisms were genotyped by pyrosequencing in 33 F0, 70 F1, 418 F2, 86 F3 and 128 individuals coming from the backcross of four F2 males with 24 Landrace females. Thirteen microsatellites and one SNP were also genotyped. Traits analysed were: backfat thickness at different locations (BF(T)), intramuscular fat percentage (IMF(P)), eye muscle area (EM(A)), loin depth (LO(D)), weight of shoulder (SH(W)), weight of ribs (RIB(W)) and weight of belly bacon (BB(W)). Different statistical models were applied in order to evaluate the number and effects of QTL on chromosome 6 and the possible causality of the LEPR gene variants with respect to the QTL. The results support the presence of two QTL on SSC6. One, at position 60-100 cM, affects BF(T) and RIB(W). The other and more significant maps in a narrow region (130-132 cM) and affects BF(T), IMF(P), EM(A), LO(D), SH(W), RIB(W) and BB(W). Results also support the association between LEPR alleles and BF(T) traits. The possible functional implications of the analysed polymorphisms are considered.
authors.affiliation: Departamento de Mejora Genética Animal, SGIT-INIA, 28040 Madrid, Spain. ovilo@inia.es
authors.forename: C
authors.initials: C
authors.lastname: Ovilo
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.forename: J L
authors.initials: JL
authors.lastname: Noguera
authors.forename: C
authors.initials: C
authors.lastname: Barragán
authors.forename: R
authors.initials: R
authors.lastname: Letón
authors.forename: C
authors.initials: C
authors.lastname: Rodríguez
authors.forename: A
authors.initials: A
authors.lastname: Mercadé
authors.forename: E
authors.initials: E
authors.lastname: Alves
authors.forename: J M
authors.initials: JM
authors.lastname: Folch
authors.forename: L
authors.initials: L
authors.lastname: Varona
authors.forename: M
authors.initials: M
authors.lastname: Toro
mesh_terms: D000483:Alleles; D000818:Animals; D001823:Body Composition; D002874:Chromosome Mapping / Q000379:methods*; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000378:metabolism; D008040:Genetic Linkage; D005838:Genotype; D006239:Haplotypes; D016013:Likelihood Functions; D018895:Microsatellite Repeats; D008957:Models, Genetic; D020125:Mutation, Missense; D010641:Phenotype; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D013552:Swine
chemical_list: D018076:DNA, Complementary