title: Novel antibacterial proteins from entomocidal crystals of Bacillus thuringiensis ssp. israelensis.
abstract: Proteins with molecular masses of 36 and 34 kDa (Bti36 and Bti34) were isolated from entomocidal crystals formed by Bacillus thuringiensis ssp. israelensis cells. The samples of Bti36 contained the admixture of a protein with a molecular mass of 33 kDa (Bti33), apparently a product of proteolysis of Bti36. These 3 proteins are significantly different in N-terminal sequences from known delta-endotoxins of B. thuringiensis and show antibacterial activity toward Micrococcus luteus. The combination of Bti36 and Bti33 also suppresses the growth of some other microorganisms including Streptomyces chrysomallus. The effects of the mixture of Bti36 and Bti33 on the M. luteus cell surface and on the surface of S. chrysomallus cells and exospores are similar, but they are different from the effect of endotoxin Cry11A on micrococcal cells.
authors.affiliation: The Scientific Research Institute for Genetics and Selection of Industrial Microorganisns, 1st Dorozhny proezd 1, Moscow 117545, Russia.
authors.forename: Lyudmila P
authors.initials: LP
authors.lastname: Revina
authors.forename: Lyubov I
authors.initials: LI
authors.lastname: Kostina
authors.forename: Maria A
authors.initials: MA
authors.lastname: Dronina
authors.forename: Igor A
authors.initials: IA
authors.lastname: Zalunin
authors.forename: Galina G
authors.initials: GG
authors.lastname: Chestukhina
authors.forename: Tatyana G
authors.initials: TG
authors.lastname: Yudina
authors.forename: Anna V
authors.initials: AV
authors.lastname: Konukhova
authors.forename: Anna V
authors.initials: AV
authors.lastname: Izumrudova
mesh_terms: D000330:Aedes / Q000187:drug effects; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001413:Bacillus thuringiensis / Q000378:metabolism* / Q000502:physiology; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001427:Bacterial Toxins / Q000737:chemistry*; D004731:Endotoxins / Q000737:chemistry*; D006460:Hemolysin Proteins; D008826:Microbial Sensitivity Tests; D016982:Micrococcus luteus / Q000187:drug effects* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D013171:Spores, Bacterial / Q000502:physiology / Q000648:ultrastructure; D013302:Streptomyces / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis