Rosmarinic acid content and RAPD analysis of in vitro regenerated basil (Ocimum americanum) plants.
Various in vitro cultures were established from shoot tips of Ocimum americanum seedlings. Rosmarinic acid content of the in vitro produced plants as well as parent plant were determined by HPLC analysis and subjected to RAPD analysis. MS medium with BA at a concentration of 1 mg/l and 0.25 mg/l IAA supports maximum rosmarinic acid production in plants produced from cultures grown on that medium. RAPD analysis revealed 64 scorable bands from four primers, including six polymorphic bands. The band pattern revealed differences between the parent plant and the in vitro regenerated plants. Certain band changes were found in O. americanum plants regenerated in vitro, suggesting the existence of genetic variation that might affect the biochemical synthesis of plants derived from tissue culture.
Rady
Mohamed R
MR
Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre, El-Tahrir St., 12311 Dokki, Giza, Egypt. drrady1@yahoo.com
Nazif
Naglaa M
NM
D018929:Cell Culture Techniques; D002934:Cinnamates / Q000378:metabolism*; D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis; D053630:Depsides; D006801:Humans; D031339:Ocimum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D008517:Phytotherapy; D018520:Plant Shoots / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D000097123:Rosmarinic Acid
D002934:Cinnamates; D017931:DNA Primers; D018744:DNA, Plant; D053630:Depsides; D000097123:Rosmarinic Acid