[Studies on the nasal epithelium toxicity of adjuvants and recombination hirudin (rHV2) nasal spary].
OBJECTIVE
To investigate the nasal epithelium toxicity of adjuvants and rHV2 nasal spary(HVS).


METHODS
Ciliary movement were evaluated with in situ toad palate model; The histology assessment of nasal epithelium were carried out after long-lasting and repeated use of HVS.


CONCLUSIONS
Adjuvants included SDS, Brij 35, azone, lecithin, EDTA, menthol, nipagin and thiomersal were able to significantly inhibited the ciliary movement, while tween80, glycyrrhizic acid monoammonium salt, benzalkonium bromide, sodium benzoate and adhensive materials investigated had less influence on it. HVS was able to damaged the nasal epithelium, but this effect recovered soon after stopping administration. It was demonstrated that SDS, Brij 35, azone,lecithin, EDTA, menthol, nipagin and thiomersal. It had significant cilitoxity, while tween80, glycyrrhizic acid monoammonium salt, benzalkonium bromide, sodium benzoate and adhensive materials investigated had no significance; Chitosan co-administration with some adjuvants may make the cillitoxity severer; It is available that rHV2 be administered by nasal spary.
Zhang
Yu-Jie
YJ
School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, China.
Hou
Jun-Ling
JL
Ma
Chang-Hua
CH
Wang
Xiao-Liang
XL
Chen
Ming-Xia
MX
Zhang
Qiang
Q
D000277:Adjuvants, Pharmaceutic / Q000008:administration & dosage / Q000633:toxicity*; D000281:Administration, Intranasal; D000818:Animals; D002023:Bufo bufo; D048271:Chitosan / Q000008:administration & dosage / Q000633:toxicity; D002923:Cilia / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects; D005260:Female; D006629:Hirudins / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D009297:Nasal Mucosa / Q000187:drug effects*; D010159:Palate / Q000187:drug effects; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000633:toxicity
D000277:Adjuvants, Pharmaceutic; D006629:Hirudins; D011994:Recombinant Proteins; C072299:hirudin HV2; D048271:Chitosan