title: Multi-residue method for the determination of thyreostats in urine samples using liquid chromatography coupled to tandem mass spectrometry after derivatisation with 3-iodobenzylbromide.
abstract: Thyreostats are banned compounds in Europe since 1981 (directive 81/602/EC) because of their carcinogenic and teratogenic properties. The control of their illegal use in breeding animals is amongst the most difficult because of their low molecular weight, high polarity and the existence of tautomeric forms. The analytical procedure described in this paper involves, after a derivatisation step with 3-iodobenzylbromide, the detection and identification at low level (microg/L) by LC-ESI(-)-MS/MS of eight thyreostats in various biological matrices (urine, muscle, liver, thyroid, animal feed, faeces and hair). The urine method has been validated according to the EU criteria (2002/657/EC decision): CCalpha and CCbeta were found in the range (0.1-5.2 microg/L) and (2.6-23.2 microg/L), respectively. The performances fulfil the requirements of the EU regarding the provisional minimum required performance limit (MRPL) fixed at 100 microg/L in urine.
authors.affiliation: Laboratoire d'Etude des Résidus et Contaminants dans les Aliments, Ecole Nationale Vétérinaire de Nantes, France. pinel@vet-nantes.fr
authors.forename: Gaud
authors.initials: G
authors.lastname: Pinel
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Bichon
authors.forename: Karine
authors.initials: K
authors.lastname: Pouponneau
authors.forename: Daniel
authors.initials: D
authors.lastname: Maume
authors.forename: François
authors.initials: F
authors.lastname: André
authors.forename: Bruno
authors.initials: B
authors.lastname: Le Bizec
mesh_terms: D000818:Animals; D013956:Antithyroid Agents / Q000032:analysis* / Q000652:urine; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry; D007462:Iodobenzenes / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine
chemical_list: D013956:Antithyroid Agents; D007462:Iodobenzenes