[Optical detection system for micro biochemical analyses].
For the need of biochemical chip, which consumes fewer specimens and is easy to integrate with micro-fluid chip, two kinds of spectrophotometric analysis methods are described in the present paper. Both the direct detection method and evanescent wave detection method are used in the experiments with visible light (460-800 nm). The experimental results proved that the direct detection is simple and evident; on the other hand the evanescent wave detection method consumes much less reagent and is easy to integrate with microchips.
Li
Feng
F
Changchun Institute of Optics, Fine Mechanics and Physics, State Key Lab of Applied Optics, Changchun 130033, China.
Wu
Yi-hui
YH
Zhao
Hua-bing
HB
Ju
Hui
H
D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D055096:Optical Devices; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation; D013053:Spectrophotometry / Q000295:instrumentation* / Q000379:methods