title: Live attenuated bluetongue vaccine viruses in Dorset Poll sheep, before and after passage in vector midges (Diptera: Ceratopogonidae).
abstract: The aim of this study was to address concerns associating with the use of BTV attenuated commercial vaccines in European sheep. These concerns include development of viraemia, possibility of transmission by vectors, reversion to virulence and re-assortment with wild-type viruses. The two vaccine viruses (BTV 2 and 9) replicated in two species of Culicoides subsequent to oral infection reaching titres suggesting transmission would occur. Viraemia in Dorset Poll sheep inoculated with either vaccine or insect passaged vaccine viruses persisted for up to 17 days, recording titres that ranged from 2.5 to 6.25 log(10)TCID(50)/ml, which is easily sufficient to infect vector Culicoides. Moderate to severe clinical signs of BT, albeit short lived, were observed in sheep following vaccination. However, to date there is no evidence of increasing virulence following two sequential passages through the vectors.
authors.affiliation: Institute for Animal Health, Department of Arbovirology, Ash Road, Pirbright, Woking, Surrey GU24 ONF, UK. eva.veronesi@bbsrc.ac.uk
authors.forename: E
authors.initials: E
authors.lastname: Veronesi
authors.forename: C
authors.initials: C
authors.lastname: Hamblin
authors.forename: P S
authors.initials: PS
authors.lastname: Mellor
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000635:transmission*; D001820:Bluetongue virus / Q000302:isolation & purification / Q000502:physiology*; D002519:Ceratopogonidae / Q000821:virology*; D004199:Disease Vectors; D007928:Lethal Dose 50; D012756:Sheep; D014613:Vaccines, Attenuated / Q000008:administration & dosage*; D014766:Viremia / Q000635:transmission / Q000662:veterinary* / Q000821:virology
chemical_list: D014613:Vaccines, Attenuated