title: Isolation and molecular typing of Naegleria fowleri from the brain of a cow that died of primary amebic meningoencephalitis.
abstract: Naegleria fowleri causes an acute and rapidly fatal central nervous system infection called primary amebic meningoencephalitis (PAM) in healthy children and young adults. We describe here the identification of N. fowleri isolated from the brain of one of several cows that died of PAM based on sequencing of the internal transcribed spacers, including the 5.8S rRNA genes.
authors.affiliation: Division of Parasitic Diseases, MS-F-13, Chamblee Campus, Centers for Diseases Control and Prevention, Atlanta, GA 30341-3724, USA. gsv1@cdc.gov
authors.forename: Govinda S
authors.initials: GS
authors.lastname: Visvesvara
authors.forename: Johan F
authors.initials: JF
authors.lastname: De Jonckheere
authors.forename: Rama
authors.initials: R
authors.lastname: Sriram
authors.forename: Barbara
authors.initials: B
authors.lastname: Daft
mesh_terms: D000562:Amebiasis / Q000469:parasitology / Q000662:veterinary*; D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D001921:Brain / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D020808:Central Nervous System Protozoal Infections / Q000469:parasitology / Q000662:veterinary*; D005260:Female; D008590:Meningoencephalitis; D008969:Molecular Sequence Data; D016848:Naegleria fowleri / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*