Reduced T cell response to beta-lactoglobulin by conjugation with acidic oligosaccharides.
We have previously reported that the conjugation of beta-lactoglobulin (beta-LG) with alginic acid oligosaccharide (ALGO) and phosphoryl oligosaccharides reduced the immunogenicity of beta-LG. In addition, those conjugates showed higher thermal stability and improved emulsifying properties than those of native beta-LG. We examine in this study the effect of conjugation on the T cell response. Our results demonstrate that the T cell response was reduced when mice were immunized with the conjugates. The findings obtained from an experiment using overlapping synthetic peptides show that novel epitopes were not generated by conjugation. One of the mechanisms for the reduced T cell response to the conjugates was found to be the reduced susceptibility of the conjugates to processing enzymes for antigen presentation. We further clarify that the beta-LG-ALGO conjugate modulated the immune response to Th1 dominance. We consider that this property of the beta-LG-ALGO conjugate would be effective for preventing food allergy as well as by its reduced immunogenicity. Our observations indicate that the method used in this study could be applied to various protein allergens to achieve reduced allergenicity with multiple improvements in their properties.
Yoshida
Tadashi
T
Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Sasahara
Yoshimasa
Y
Miyakawa
Shunpei
S
Hattori
Makoto
M
D000464:Alginates / Q000737:chemistry; D000485:Allergens / Q000276:immunology*; D000818:Animals; D005260:Female; D005512:Food Hypersensitivity / Q000517:prevention & control; D020723:Glucuronic Acid / Q000737:chemistry / Q000276:immunology; D006603:Hexuronic Acids / Q000737:chemistry / Q000276:immunology; D007114:Immunization; D007782:Lactoglobulins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology
D000464:Alginates; D000485:Allergens; D006603:Hexuronic Acids; D007782:Lactoglobulins; D009844:Oligosaccharides; D020723:Glucuronic Acid