Interactions of charged ligands with beta(2)-microglobulin conformers in affinity capillary electrophoresis.
Alternative conformations of beta(2)-microglobulin (beta(2)m) are involved in its transformation from soluble monomeric precursor molecules to the insoluble polymeric material that constitutes beta(2)m amyloid. Accordingly, non-native conditions such as low pH or high ionic strength promote beta(2)m amyloid formation in vitro. The early events in these processes are not well known, partly because of the paucity of techniques available for the characterization of transient folding intermediates in proteins. We have used high-resolution separations in capillaries (capillary electrophoresis, CE) to resolve putative conformer fractions in native and structurally modified beta(2)m and to show the induction of alternatively folded beta(2)m under different experimental conditions. The conformer fractions are observed as distinct peaks in the separation profiles and thus it is possible to probe for the reactivity of these individual beta(2)m species with specific ligands that, upon binding, alter analyte mobility in affinity capillary electrophoresis experiments. Interactions were shown in this way for the negatively charged substances heparin, Congo red, and suramin, as well as for Cu(2+) ions. Marked differences in the binding behavior of the beta(2)m conformational variants compared with native beta(2)m could be demonstrated. This approach for conformer separation and binding characterization is a valuable starting point for the assessment of various ligand molecules, or analogues thereof, as agents capable of perturbing the mechanisms of fibril formation.
Heegaard
Niels H H
NH
Department of Autoimmunology, Statens Serum Institute, Bldg. 81, Rm. 536, Artillerivej 5, DK-2300 Copenhagen S, Denmark. nhe@ssi.dk
De Lorenzi
Ersilia
E
D000682:Amyloid / Q000096:biosynthesis*; D003224:Congo Red / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D019075:Electrophoresis, Capillary; D006493:Heparin / Q000737:chemistry; D008024:Ligands; D009154:Mutation; D011487:Protein Conformation; D017510:Protein Folding; D013498:Suramin / Q000737:chemistry; D001613:beta 2-Microglobulin / Q000737:chemistry* / Q000235:genetics
D000682:Amyloid; D008024:Ligands; D001613:beta 2-Microglobulin; D003224:Congo Red; D013498:Suramin; D003300:Copper; D006493:Heparin; C029892:cupric chloride