title: Evaluation of beta-lactamase inhibitors in disk tests for detection of plasmid-mediated AmpC beta-lactamases in well-characterized clinical strains of Klebsiella spp.
abstract: The diagnostic utility of the AmpC beta-lactamase inhibitors LN-2-128, 48-1220, and Syn 2190 in combination with cefotetan (CTT) or cefoxitin in a disk test for the detection of clinical isolates of Klebsiella spp. producing plasmid-mediated AmpC beta-lactamases (pAmpCs) was evaluated. The combination of Syn 2190 and CTT had a sensitivity of 91%, a specificity of 100%, and a reproducibility of 100% and showed the best potential of using an inhibitor for detection of Klebsiella spp. producing pAmpCs.
authors.affiliation: Center for Research in Anti-infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska, USA.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Black
authors.forename: Kenneth S
authors.initials: KS
authors.lastname: Thomson
authors.forename: John D
authors.initials: JD
authors.lastname: Buynak
authors.forename: Johann D D
authors.initials: JD
authors.lastname: Pitout
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000037:antagonists & inhibitors; D015313:Cefotetan / Q000494:pharmacology; D002440:Cefoxitin / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D007709:Klebsiella / Q000187:drug effects / Q000201:enzymology*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D065093:beta-Lactamase Inhibitors; D015313:Cefotetan; D002440:Cefoxitin; C048795:AmpC beta-lactamases; D001618:beta-Lactamases