Peptidoglycan precursor pools associated with MraY and FtsW deficiencies or antibiotic treatments.
Blocking peptidoglycan synthesis in Escherichia coli with moenomycin or vancomycin led to the accumulation of UDP-MurNAc-pentapeptide and of its immediate upstream precursors, whereas with cephaloridine or penicillin G the pool of UDP-MurNAc-pentapeptide decreased. With MraY and FtsW deficiencies the decrease of UDP-MurNAc-pentapeptide was accompanied by an increase of the upstream nucleotide precursors and the appearance of UDP-MurNAc-tetrapeptide.
Lara
Beatriz
B
Enveloppes Bactériennes et Antibiotiques, UMR 8619 CNRS, Bâtiment 430, Université Paris-Sud, 91405 Orsay, France.
Mengin-Lecreulx
Dominique
D
Ayala
Juan A
JA
van Heijenoort
Jean
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002240:Carbohydrate Sequence; D002509:Cephaloridine / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000494:pharmacology; D010400:Penicillin G / Q000494:pharmacology; D010457:Peptidoglycan / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D014166:Transferases / Q000235:genetics*; D017855:Transferases (Other Substituted Phosphate Groups); D014538:Uridine Diphosphate N-Acetylmuramic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D014640:Vancomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008565:Membrane Proteins; D009844:Oligosaccharides; D010457:Peptidoglycan; D011498:Protein Precursors; D014538:Uridine Diphosphate N-Acetylmuramic Acid; C061684:FtsW protein, Bacteria; C002225:UDP-N-acetylmuramic acid pentapeptide; D014640:Vancomycin; D014166:Transferases; D017855:Transferases (Other Substituted Phosphate Groups); C079803:mraY protein, Bacteria; D002509:Cephaloridine; C419802:moenomycin; D010400:Penicillin G