title: Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides.
abstract: Absolute quantification in proteomics usually involves simultaneous determination of representative proteolytic peptides and stable isotope-labeled analogs. The principal limitation to widespread implementation of this approach is the availability of standard signature peptides in accurately known amounts. We report the successful design and construction of an artificial gene encoding a concatenation of tryptic peptides (QCAT protein) from several chick (Gallus gallus) skeletal muscle proteins and features for quantification and purification.
authors.affiliation: Department of Veterinary Preclinical Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZJ, UK. r.beynon@liv.ac.uk
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Beynon
authors.forename: Mary K
authors.initials: MK
authors.lastname: Doherty
authors.forename: Julie M
authors.initials: JM
authors.lastname: Pratt
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Gaskell
mesh_terms: D000818:Animals; D002645:Chickens; D020869:Gene Expression Profiling / Q000379:methods*; D009124:Muscle Proteins / Q000032:analysis* / Q000378:metabolism; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D009124:Muscle Proteins; D010455:Peptides; D020543:Proteome