Analysis of recombinant group B streptococcal protein ScaAB and evaluation of its immunogenicity.
Group B streptococcal (GBS) gene encoding the putative lipoprotein and adherence factor ScaAB was cloned and expressed in E. coli. Recombinant ScaAB protein was isolated. Signal sequence of ScaAB was found to be cleaved in the E. coli host. ScaAB recombinant protein was immunogenic in mice and antibodies against this protein were discovered in mice sera after GBS infection. The perspectives of the use of ScaAB protein in GBS vaccine are discussed.
Vorobieva
E I
EI
Institute of Experimental Medicine, Russian Academy of Medical Sciences, 197 376 St. Petersburg, Russia.
Meringova
L F
LF
Leontieva
G F
GF
Grabovskaya
K B
KB
Suvorov
A N
AN
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007114:Immunization; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013290:Streptococcal Infections / Q000382:microbiology / Q000401:mortality / Q000517:prevention & control*; D013292:Streptococcus agalactiae / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins