Chitinase induces lysis of MCF-7 cells in culture and of human breast cancer xenograft B11-2 in SCID mice.
BACKGROUND
It has long been known that the polycarbohydrates on the neoplastic cell surface are different from those on normal cells; differences which allow one to attack tumor cells selectively. Although the exact differences between tumor cells and normal cells are still not clearly known, research into these differences is ongoing for anticancer drug development.


METHODS
The human breast cancer cell line MCF-7 in culture and human breast xenograft B(11)-2 in SCID mice were used in our observations. Two different samples of chitinase from different bacteria were tested in the experiments. Optical observation of regular H & E-stained tumor tissue sections and observations by transmission electron microscopic techniques were used in this study.


RESULTS
MCF-7 breast cancer cells in culture showed structural damage within 7 hours after 1.3 unit/ml of chitinase was added to the medium, while normal mice spleen cells did not. The transplanted B(11)-2 xenograft tissue in mice started to lyse 12 hours after chitinase was injected; the size of the tumor gradually reduced and finally a scab was formed, which came off the skin a few days later. All the tested tumor-bearing mice survived and these cured mice had no tumor re-growth during the following 1-year observation period.


CONCLUSIONS
Chitinase selectively lysed the tumor cells in vitro and in vivo. Injected chitinase destroyed the tumor tissue and cured the mice. The further development of this type of treatment and of the mechanisms of chitinase action are discussed.
Pan
Xing Q
XQ
College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA. pan.67@osu.edu
Shih
Chu C
CC
Harday
John
J
D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002688:Chitinases / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009336:Necrosis; D023041:Xenograft Model Antitumor Assays
D002688:Chitinases