Effect of methylmercury on some neurotransmitters and oxidative damage of rats.
In order to study the molecular mechanism of injury in rat organs induced by methylmercury, and the relationship between neurotransmitter and oxidative damage in the toxicity process of rat injury by methylmercury was studied. The control group was physiological saline of 0.9%, the concentration of exposure groups were 5 mg/( kg x d) and 10 mg/( kg x d) respectively. The content of AChE, ACh, NOS, NO, MDA, SOD, GSH-Px and GSH in different organs of rats were determined with conventional methods. The results showed that after exposure to methylmercury for 7 d, the mercury content in brain of exposure groups increased clearly and had significant difference compared with the control group (P < 0.01). In rat's brain, serum, liver and kidney, the content of ACh and AChE were all decreased; the content of NOS and NO were all increased; the content of MDA was increased compared with the control group, the exposure groups had significant difference (P < 0.01); the content of SOD, GSH and GSH-Px was decreased compared with the control group, the exposure groups had significant difference (P < 0.01). It could be concluded that methylmercury did effect the change of neurotransmitter and free radical. They participated in the toxicity process of injury by methylmercury. The damage of neurotransmitter maybe cause the chaos of free radical and the chaos of free radical may also do more damage to neurotransmitter vice versa.
Cheng
Jin-Ping
JP
School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, China. jpcheng@sjtu.edu.cn
Yang
Yi-Chen
YC
Hu
Wei-Xuan
WX
Yang
Liu
L
Wang
Wen-Hua
WH
Jia
Jin-Ping
JP
Lin
Xue-Yu
XY
D000109:Acetylcholine / Q000097:blood / Q000378:metabolism; D000110:Acetylcholinesterase / Q000097:blood / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008767:Methylmercury Compounds / Q000633:toxicity*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009569:Nitric Oxide / Q000097:blood / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000097:blood / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats / Q000378:metabolism*; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
D008767:Methylmercury Compounds; D018377:Neurotransmitter Agents; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase; D005978:Glutathione; D000109:Acetylcholine