Erythrocyte adaptation to oxidative stress in endurance training.
BACKGROUND
We tested the hypothesis that endurance training may reduce exercise oxidative stress damage on erythrocytes.


METHODS
Fifteen subjects performed a standardized endurance exercise at 75% of maximal oxygen consumption weekly during a 19-week training period. Blood samples taken before and after exercise were analyzed by Fourier transform-infrared (FT-IR) spectrometry to determine exercise-induced change in plasma concentrations and erythrocyte IR absorptions.


RESULTS
Training first induced a stabilization of plasma concentration changes during exercise (unchanged for glucose, increased for lactate, triglycerides, glycerol, and fatty acids), whereas erythrocyte phospholipid alterations remained elevated (p <0.05). Further, training reduced the exercise-induced erythrocyte lactate content increase (nuC-O; p <0.05) and phospholipid alterations (nuC-H(n) and nuP=O; p <0.05) during exercise. These changes paralleled the decrease of exercise-induced hemoconcentration (p <0.05) and plasma lactate increase (p <0.05).


CONCLUSIONS
These correlated changes between plasma and erythrocyte parameters suggest that endurance training reduces erythrocyte susceptibility to oxidative stress.
Petibois
Cyril
C
Groupe de Chimie Bio-Organique, Université Victor Segalen Bordeaux 2, France. cyril.petibois@u-bordeaux2.fr
Déléris
Gérard
G
D000222:Adaptation, Physiological; D000328:Adult; D004032:Diet; D004912:Erythrocytes / Q000737:chemistry / Q000201:enzymology / Q000502:physiology*; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D010101:Oxygen Consumption; D010807:Physical Endurance; D010949:Plasma / Q000737:chemistry / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000378:metabolism
D013482:Superoxide Dismutase