title: Plasma tissue factor plus activated peripheral mononuclear cells activate factors VII and X in cardiac surgical wounds.
abstract: OBJECTIVE
  The purpose of this study was to test the hypothesis that activated monocytes with soluble plasma tissue factor (pTF) activate factors VII and X to generate thrombin.
  
  
  BACKGROUND
  Despite heparin, thrombin is progressively generated during cardiac surgery with cardiopulmonary bypass (CPB), produces intravascular fibrin and fibrinolysis, and causes serious thromboembolic and nonsurgical bleeding complications. Thrombin is primarily produced in the surgical wound, but mechanisms are unclear.
  
  
  METHODS
  In 13 patients, interactions of mononuclear cells, platelets, pTF, and pTF fractions to activate factors VII and X were evaluated in pre-bypass, perfusate, and pericardial wound blood before and during CPB.
  
  
  RESULTS
  Monocytes are activated in wound, but not in pre-bypass or perfusate plasma (monocyte chemotactic protein-1 = 29.5 +/- 2.1 pmoles/l vs. 2.8 +/- 1.2 pmoles/l and 3.3 +/-1.4 pmoles/l, respectively). Wound pTF is substantially elevated compared to other locations (3.64 +/- 0.45 pmoles/l vs. 0.71 +/- 0.65 pmoles/l and 1.31 +/- 1.4 pmoles/l). Supernatant wound pTF contains 81.7% of TF antigen; wound microparticle pTF contains 18.3%. Wound monocytes and all C5a-stimulated monocytes (but not activated platelets) completely convert factor VII to factor VIIa with wound pTF. Activated monocytes more efficiently activate factor X with wound supernatant TF/factor VII(VIIa) complex than with wound microparticle TF/factor VII(fVIIa). The correlation coefficient (r) between wound thrombin generation (F1.2) and wound pTF concentration is 0.944 (p = 0.0004).
  
  
  CONCLUSIONS
  During cardiac surgery with CPB, wound monocytes plus wound pTF or wound microparticle-free supernatant pTF preferentially accelerate activation of factor VII and factor X. This system represents a novel mechanism for thrombin generation via the TF coagulation pathway.
authors.affiliation: Harrison Department of Surgical Research, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hattori
authors.forename: Mohammad M H
authors.initials: MM
authors.lastname: Khan
authors.forename: Robert W
authors.initials: RW
authors.lastname: Colman
authors.forename: L Henry
authors.initials: LH
authors.lastname: Edmunds
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001777:Blood Coagulation / Q000502:physiology*; D001779:Blood Coagulation Factors / Q000502:physiology; D015153:Blotting, Western; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D005167:Factor VII / Q000032:analysis / Q000096:biosynthesis*; D005170:Factor X / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology*; D010949:Plasma / Q000737:chemistry*; D011182:Postoperative Care; D019106:Postoperative Hemorrhage / Q000503:physiopathology*; D013917:Thrombin / Q000096:biosynthesis*; D013925:Thromboplastin / Q000032:analysis*; D014945:Wound Healing
chemical_list: D001779:Blood Coagulation Factors; C045071:leukocyte procoagulant activity; D005167:Factor VII; D005170:Factor X; D013925:Thromboplastin; D013917:Thrombin