title: [Demonstration of simultaneous infection with dengue type 2 and 3 in a Chinese patient].
abstract: IgG and IgM antibodies to dengue virus(DEN) in patient serum sample were detected with an indirect immunofluorescence assay. The patient's serum was inoculated into the culture of C6/36 cells to isolate dengue virus. Viral RNA was extracted from cultured supernatant and RT-PCR amplification and sequencing were carried out. The result showed that there were anti-dengue virus IgG and IgM antibodies in the serum. Both dengue type 2 and 3 virus specific sequences were confirmed by RT-PCR and sequence analysis. The results presented here demonstrated the simultaneous infection with dengue type 2 and 3 viruses in the Chinese patient.
authors.affiliation: Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. yu_man@sohu.com
authors.forename: Man
authors.initials: M
authors.lastname: Yu
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Peng
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Fan
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Deng
authors.forename: E-De
authors.initials: ED
authors.lastname: Qin
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D003588:Cytopathogenic Effect, Viral; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000302:isolation & purification; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M