Analysis of polymorphic sites in the promoter of the nitric oxide synthase 2 gene.
A point mutation (G --> C) in the gene promoter for the human nitric oxide synthase (NOS) 2 at position -954 is associated with protection against severe Plasmodium falciparum malaria in Gabon. Carriers of this mutation show higher basal levels of nitric oxide production than wild type individuals. To obtain information about the possible binding transcription factors, nucleic proteins from the lung carcinoma cell line were enriched by affinity chromatography using DEAE-Sepharose and immobilized oligonucleotides derived from the promoter sequence. A mutational analysis was performed on 30 samples to detect polymorphisms in the NOS2 promoter region that contains important NF-kappaB sites. Three point mutations were identified in this region. In vitro studies with promoter constructs showed an altered expression of the marker gene depending on the promoter variant used.
Coia
Valentina
V
Institute for Tropical Medicine, Department of Parasitology, University of Tübingen, Wilhelmstr. 27, 72074 Tübingen, Germany.
Jüliger
Simone
S
Mordmüller
Benjamin
B
Kreidenweis
Andrea
A
Stroh
Anna-Lena
AL
Ortega
Claudia
C
Vindigni
Alessandro
A
Dengjel
Jörn
J
Lukyanov
Dmitry V
DV
Destro-Bisol
Giovanni
G
Fedorov
Anton
A
Podgornaya
Olga I
OI
Kun
Jürgen F J
JF
D001483:Base Sequence; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
D029742:Caenorhabditis elegans Proteins; C400703:nos-2 protein, C elegans