Protective effect of green tea polyphenols on the SH-SY5Y cells against 6-OHDA induced apoptosis through ROS-NO pathway.
Green tea polyphenols (GTP) are thought to help prevent oxidative stress-related diseases, such as cancer, cardiovascular disease, neurodegenerative disease, and aging. We here investigate the protective mechanisms of GTP on SH-SY5Y cells against apoptosis induced by the pro-parkinsonian neurotoxin 6-hydroxydopamine (6-OHDA). GTP rescued the changes in condensed nuclear and apoptotic bodies, attenuated 6-OHDA-induced early apoptosis, prevented the decrease in mitochondrial membrane potential, and suppressed accumulation of reactive oxygen species (ROS) and of intracellular free Ca(2+). GTP also counteracted the 6-OHDA-induced nitric oxide increase and overexpression of nNOS and iNOS, and decreased the level of protein-bound 3-nitrotyrosine (3-NT). In addition, GTP inhibited the autooxidation of 6-OHDA and scavenged oxygen free radicals in a dose- and time-dependent manner. Our results show that the protective effects of GTP on SH-SY5Y cells are mediated, at least in part, by controlling the ROS-NO pathway.
Guo
Shuhong
S
Laboratory of Visual Information processing, Center of Brain & Cognitive Science, Institute of Biophysics, Academia Sinica, Beijing 100101, People's Republic of China.
Bezard
Erwan
E
Zhao
Baolu
B
D017304:Annexin A5 / Q000737:chemistry; D017209:Apoptosis; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival; D004396:Coloring Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D005434:Flow Cytometry; D005609:Free Radicals; D006160:Guanosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D009474:Neurons / Q000378:metabolism; D009569:Nitric Oxide / Q000737:chemistry / Q000378:metabolism; D016627:Oxidopamine / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D010300:Parkinson Disease / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D011809:Quinones / Q000378:metabolism; D017382:Reactive Oxygen Species; D013662:Tea; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism
D017304:Annexin A5; D004396:Coloring Agents; D005419:Flavonoids; D005609:Free Radicals; D010636:Phenols; D059808:Polyphenols; D011809:Quinones; D017382:Reactive Oxygen Species; D013662:Tea; D013778:Tetrazolium Salts; D013844:Thiazoles; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D006160:Guanosine Triphosphate; D016627:Oxidopamine; C022616:thiazolyl blue; D010100:Oxygen; D002118:Calcium