Correlation of biological activity and reactor performance in biofiltration of toluene with the fungus Paecilomyces variotii CBS115145.
A biofiltration system inoculated with the mold Paecilomyces variotii CBS115145 showed a toluene elimination capacity (EC) of around 250 g/m3 of biofilter/h, which was higher than the values usually reported for bacteria. P. variotii assimilated m- and p-cresols but not the o isomer. Initial toluene hydroxylation occurred both on the methyl group and through the p-cresol pathway. These results were corroborated by detecting benzyl alcohol, benzaldehyde, and p-cresol as volatile intermediates. In liquid cultures with toluene as a substrate, the activity of toluene oxygenase (TO) was 5.6 nmol of O2/min/mg of biomass, and that of benzyl alcohol dehydrogenase was 16.2 nmol of NADH/min/mg of protein. Toluene biodegradation determined from the TO activity in the biofilter depended on the biomass distribution and the substrate concentration. The specific enzymatic activity decreased from 6.3 to 1.9 nmol of O2/min/mg of biomass along the reactor. Good agreement was found between the EC calculated from the TO activity and the EC measured on the biofilter. The results were confirmed by short-time biofiltration experiments. Average EC measured in different biofiltration experiments and EC calculated from the TO activity showed a linear relation, suggesting that in the biofilters, EC was limited by biological reaction. As the enzymatic activities of P. variotii were similar to those reported for bacteria, the high performance of the fungal biofilters can possibly be explained by the increased transfer of the hydrophobic compounds, including oxygen, from the gas phase to the mycelia, overcoming the transfer problems associated with the flat bacterial biofilms.
García-Peña
Inés
I
Department of Chemical Engineering, UAM-Iztapalapa, P.O. Box 55-534, 09340 Mexico City, Mexico.
Hernández
Sergio
S
Auria
Richard
R
Revah
Sergio
S
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism; D003470:Culture Media; D005374:Filtration; D010101:Oxygen Consumption; D010105:Oxygenases / Q000378:metabolism; D010143:Paecilomyces / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D014050:Toluene / Q000378:metabolism*
D003470:Culture Media; D002245:Carbon Dioxide; D014050:Toluene; D000429:Alcohol Oxidoreductases; C027016:benzyl alcohol dehydrogenase; D010105:Oxygenases