Developmental alterations as a result of in ovo exposure to the pesticide metabolite p,p'-DDE in Alligator mississippiensis.
The pesticide metabolite p,p'-DDE (1,1-dichloro-2,2-bis (p-chlorophenyl) ethylene), has been implicated in numerous reproductive and developmental abnormalities. Previous work has shown this ubiquitous contaminant to behave in an estrogenic or antiandrogenic manner, depending on the species and endpoints examined. In the current study, we examined the effects of embryonic exposure to p,p'-DDE in the alligator, a species that exhibits temperature-dependent sex determination. We compared sex ratios at an intermediate and all male producing temperature, as well as plasma testosterone (T) and gonad aromatase activity relative to untreated controls and in ovo estradiol-17beta (E(2))-treated neonates that served as positive estrogenic controls. We also compared oviductal epithelial cell height (ECH) and phallus size-estrogen and androgen responsive tissue, respectively. A female biased sex ratio was observed among hatchlings exposed to p,p'-DDE at 100 parts per billion (ppb) wet egg mass at the intermediate incubation temperature. No effect on sex determination was observed for p,p'-DDE at the all male producing temperature. Significant influence on sex determination was observed for E(2) at 100 and 0.1 ppb at the intermediate temperature and 100 ppb at the all male producing temperature. Both p,p'-DDE and E(2) failed to influence plasma T, gonadal aromatase activity, oviductal ECH, and phallus morphology at the concentrations used. Our data show that gonadal differentiation is highly sensitive to chemical perturbation relative to the other endpoints examined, and that the response to the interaction of dose and temperature should be taken into account in similar studies.
Milnes
Matthew R
MR
Department of Zoology, University of Florida, 223 Bartram Hall, Gainesville, FL 32611-8525, USA.
Bryan
Teresa A
TA
Medina
Jennifer Gates
JG
Gunderson
Mark P
MP
Guillette
Louis J
LJ
D000489:Alligators and Crocodiles / Q000196:embryology* / Q000254:growth & development*; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D003633:Dichlorodiphenyl Dichloroethylene / Q000633:toxicity*; D005260:Female; D008297:Male; D010053:Ovary / Q000187:drug effects / Q000196:embryology; D010057:Oviducts / Q000033:anatomy & histology / Q000187:drug effects; D010063:Ovum / Q000187:drug effects*; D010413:Penis / Q000033:anatomy & histology / Q000187:drug effects; D019849:Sex Determination Processes; D013737:Testis / Q000187:drug effects / Q000196:embryology; D013739:Testosterone / Q000097:blood
D013739:Testosterone; D003633:Dichlorodiphenyl Dichloroethylene; D001141:Aromatase