title: Anti-inflammatory activity of Seabuckthorn (Hippophae rhamnoides) leaves.
abstract: Immunomodulatory activity of Seabuckthorn (SBT) leaf extract was evaluated in adjuvant induced arthritis (AIA) rat model. Inflammation was induced by injecting Complete Freund's Adjuvant (CFA) in the right hind paw of rats. SBT extract was administered intraperitoneally to treat the inflammation. The extent of inflammation and treatment response was evaluated by clinical analysis, scintigraphic visualization using technitium-99m-glutathione (Tc99m-GSH) and lymphocyte proliferation. Serial evaluation was carried out on days 1, 7, 14, 21 and 28 after creation of inflammation. The Tc99m-GSH uptake in the inflamed leg was compared with the normal contralateral leg of the same animal. The measurements were done by obtaining scintigraphic images using gamma camera and an online computer. Both qualitative and quantitative evaluation of radiotracer accumulation was considered to evaluate the anti-inflammatory response. The lymphocyte proliferation study revealed cellular immunosuppression during the early phase of the disease. Administration of SBT extract on the same day or 5 days prior to inflammatory insult into the joint, significantly reduced the inflammation as compared to the untreated animals in a dose dependent manner. These observations suggest that the SBT leaf extract has a significant anti-inflammatory activity and has the potential for the treatment of arthritis.
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi-110054, India. lganju@rediffmail.com
authors.forename: Lilly
authors.initials: L
authors.lastname: Ganju
authors.forename: Yogendra
authors.initials: Y
authors.lastname: Padwad
authors.forename: Richa
authors.initials: R
authors.lastname: Singh
authors.forename: Dev
authors.initials: D
authors.lastname: Karan
authors.forename: Sudipta
authors.initials: S
authors.lastname: Chanda
authors.forename: Mohinder Kumar
authors.initials: MK
authors.lastname: Chopra
authors.forename: Parul
authors.initials: P
authors.lastname: Bhatnagar
authors.forename: Ravi
authors.initials: R
authors.lastname: Kashyap
authors.forename: Ramesh Chandra
authors.initials: RC
authors.lastname: Sawhney
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005978:Glutathione / Q000031:analogs & derivatives; D031281:Hippophae / Q000737:chemistry*; D007111:Immunity, Cellular / Q000187:drug effects; D007274:Injections, Intraperitoneal; D007596:Joints / Q000000981:diagnostic imaging / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D010936:Plant Extracts / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D011877:Radionuclide Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D013667:Technetium
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; C090285:technetium Tc 99m glutathione; D013667:Technetium; D005978:Glutathione