title: Application of polymerase chain reaction to detect adulteration of sheep's milk with goats' milk.
abstract: The polymerase chain reaction has been applied for the specific detection of goats' milk in sheep's milk using primers targeting the mitochondrial 12S ribosomal RNA gene. The use of goat-specific primers yielded a 122-bp fragment from goats' milk DNA, whereas no amplification signal was obtained in sheep's, cows', and water buffaloes' milk DNA. Polymerase chain reaction analysis of raw and heat-treated milk binary mixtures of sheep/goat enabled the specific detection of goats' milk with a sensitivity threshold of 0.1%. This study demonstrates the usefulness of the proposed polymerase chain reaction assay for authentication of milk products in routine analysis.
authors.affiliation: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: López-Calleja
authors.forename: I
authors.initials: I
authors.lastname: González
authors.forename: V
authors.initials: V
authors.lastname: Fajardo
authors.forename: I
authors.initials: I
authors.lastname: Martín
authors.forename: P E
authors.initials: PE
authors.lastname: Hernández
authors.forename: T
authors.initials: T
authors.lastname: García
authors.forename: R
authors.initials: R
authors.lastname: Martín
mesh_terms: D000818:Animals; D002020:Buffaloes; D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling; D006041:Goats / Q000235:genetics*; D006358:Hot Temperature; D008892:Milk / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000235:genetics; D012756:Sheep
chemical_list: D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D012313:RNA; D004247:DNA