title: Rapid and simple procedure for homogenizing leaf tissues suitable for mini-midi-scale DNA extraction in rice.
abstract: We describe a rapid and simple procedure for homogenizing leaf samples suitable for mini/midi-scale DNA preparation in rice. The methods used tungsten carbide beads and general vortexer for homogenizing leaf samples. In general, two samples can be ground completely within 11.3+/-1.5 sec at one time. Up to 20 samples can be ground at a time using a vortexer attachment. The yields of the DNA ranged from 2.2 to 7.6 microg from 25-150 mg of young fresh leaf tissue. The quality and quantity of DNA was compatible for most of PCR work and RFLP analysis.
authors.affiliation: Rice Division, Yeongnam Agricultural Research Institute, Milyang, Korea. gihwan@rda.go.kr
authors.forename: Gihwan
authors.initials: G
authors.lastname: Yi
authors.forename: Jun-Ho
authors.initials: JH
authors.lastname: Choi
authors.forename: Jong-Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Unggi
authors.initials: U
authors.lastname: Jeong
authors.forename: Min-Hee
authors.initials: MH
authors.lastname: Nam
authors.forename: Doh-Won
authors.initials: DW
authors.lastname: Yun
authors.forename: Moo-Young
authors.initials: MY
authors.lastname: Eun
mesh_terms: D018744:DNA, Plant / Q000302:isolation & purification*; D008722:Methods; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics; D017973:Tungsten Compounds / Q000737:chemistry
chemical_list: D018744:DNA, Plant; D017973:Tungsten Compounds; C002802:tungsten carbide