Interaction between lipid monolayers and poloxamer 188: an X-ray reflectivity and diffraction study.
The mechanism by which poloxamer 188 (P188) seals a damaged cell membrane is examined using the lipid monolayer as a model system. X-ray reflectivity and grazing-incidence x-ray diffraction results show that at low nominal lipid density, P188, by physically occupying the available area and phase separating from the lipids, forces the lipid molecules to pack tightly and restore the barrier function of the membrane. Upon compression to bilayer equivalent pressure, P188 is squeezed out from the lipid monolayer, allowing a graceful exit of P188 when the membrane integrity is restored.
Wu
Guohui
G
Department of Chemistry, the Institute for Biophysical Dynamics and the James Franck Institute, the University of Chicago, 5735 S. Ellis Avenue, Chicago, IL 60637, USA.
Majewski
Jaroslaw
J
Ege
Canay
C
Kjaer
Kristian
K
Weygand
Markus Jan
MJ
Lee
Ka Yee C
KY
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D001703:Biophysics / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism; D004583:Electrons; D008051:Lipid Bilayers / Q000737:chemistry; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D015233:Models, Statistical; D010715:Phosphatidylglycerols / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D020442:Poloxamer / Q000737:chemistry*; D011312:Pressure; D012542:Scattering, Radiation; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction / Q000379:methods*; D014965:X-Rays
D008051:Lipid Bilayers; D008055:Lipids; D008563:Membrane Lipids; D010715:Phosphatidylglycerols; D010743:Phospholipids; D013501:Surface-Active Agents; D014867:Water; D020442:Poloxamer; D015060:1,2-Dipalmitoylphosphatidylcholine; C030345:1,2-dipalmitoylphosphatidylglycerol