title: Protective effect of Toki-shakuyaku-san on amyloid beta25-35-induced neuronal damage in cultured rat cortical neurons.
abstract: Amyloid beta protein (Abeta) is the major component of senile plaques, the pathological hallmark of the neurodegeneration associated with Alzheimer's disease (AD). This study investigated the effect of Toki-shakuyaku-san (TSS), a traditional medicine, on Abeta25-35-induced neuronal death and lipid peroxidation assessed by measuring lactate dehydrogenase (LDH) and malondialdehyde (MDA), respectively. Abeta25-35 at 10 microM induced neuronal damage and increased the LDH and MDA. TSS at concentrations of 100 and 300 microg/mL significantly reduced the Abeta25-35-induced neuronal death and the lipid peroxidation. These results suggest that TSS has a protective effect against Abeta25-35-induced neuronal damage. TSS may be beneficial for the treatment of AD.
authors.affiliation: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1, Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Egashira
authors.forename: Katsunori
authors.initials: K
authors.lastname: Iwasaki
authors.forename: Yuki
authors.initials: Y
authors.lastname: Akiyoshi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takagaki
authors.forename: Izzettin
authors.initials: I
authors.lastname: Hatip-Al-Khatib
authors.forename: Kenichi
authors.initials: K
authors.lastname: Mishima
authors.forename: Kouji
authors.initials: K
authors.lastname: Kurauchi
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Ikeda
authors.forename: Michihiro
authors.initials: M
authors.lastname: Fujiwara
mesh_terms: D016229:Amyloid beta-Peptides; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D010446:Peptide Fragments; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016229:Amyloid beta-Peptides; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); C413220:toki-shakuyaku-san; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase