title: [Determination of Chinese traditional medicine activity eliminating superoxide anion radical by kinetics spectrophotometry].
abstract: A new method to determine Chinese traditional medicine activity eliminating superoxide anion radical by kinetic spectrophotometry was developed. It is shown that the optimum determination condition may be obtained when wavelength is 520 nm, the concentration of enzyme is 4 x 10(-3) microg x mL(-1), the reaction time is between 2-7 min. Vitamin C activity eliminating superoxide anion radical was determined under the condition, and the results were identical with literature. The method is used to determine IC50 of Cortex Magnoliae Officinalis and Cortex Eucommia, and they are 7.580 and 323.800 mg x L(-1) respectively.
authors.affiliation: College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China.
authors.forename: Yi-ling
authors.initials: YL
authors.lastname: Tian
authors.forename: Guan-hua
authors.initials: GH
authors.lastname: Chen
authors.forename: Tong
authors.initials: T
authors.lastname: Cui
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001205:Ascorbic Acid / Q000737:chemistry* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008516:Medicine, Chinese Traditional; D013053:Spectrophotometry / Q000379:methods*; D013481:Superoxides / Q000037:antagonists & inhibitors / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D013481:Superoxides; D001205:Ascorbic Acid