title: Protein expression profiling identifies maspin and stathmin as potential biomarkers of adenoid cystic carcinoma of the salivary glands.
abstract: Adenoid cystic carcinoma (ACC) is one of the most common malignant tumors of the salivary glands. It tends to grow slowly but is associated with a poor prognosis compared to other malignant salivary gland tumors. To identify specific markers of ACC, we examined protein expression profiling in ACC xenograft and normal salivary glands (NSG) using fluorescent 2-dimensional differential in-gel electrophoresis (2-D-DIGE), an emerging technique for comparative proteomics, that improves the reproducibility and reliability of differential protein expression analysis between the samples. To identify the proteins, matrix-assisted laser desorption/ionization time-of-flight peptide mass fingerprinting was carried out. Using these strategies, we detected 4 upregulated proteins and 5 downregulated proteins in ACC xenograft. Maspin and stathmin were selected for further analyses. Western blotting and immunohistochemical staining showed a higher expression of these proteins in ACC xenograft and clinical ACC tissue compared to NSG. Furthermore, Expression of these proteins was correlated with the histologic grading of ACC (n = 10). Therefore, our data indicate that maspin and stathmin may be not only useful biomarkers of ACC but also markers of biologic behavior in this tumor.
authors.affiliation: Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, Chiba, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Nakashima
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Uzawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kasamatsu
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Koike
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Endo
authors.forename: Kengo
authors.initials: K
authors.lastname: Saito
authors.forename: Susumu
authors.initials: S
authors.lastname: Hashitani
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Numata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Urade
authors.forename: Hideki
authors.initials: H
authors.lastname: Tanzawa
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000556:secondary; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D040901:Proteomics; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D012469:Salivary Glands / Q000378:metabolism*; D015843:Serpins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D050777:Stathmin / Q000378:metabolism*; D014183:Transplantation, Heterologous
chemical_list: D014408:Biomarkers, Tumor; C085344:SERPIN-B5; D015843:Serpins; D050777:Stathmin