Lotus burttii takes a position of the third corner in the lotus molecular genetics triangle.
In order to consolidate molecular genetic system in Lotus japonicus and to further access the biological diversity in Lotea, we introduce here Lotus burttii B-303 derived from West Pakistan as the third crossing partner of the Gifu ecotype (B-129-S9) for a genetic analysis. L. burttii is a relatively small and early flowering plant with non-shattering behavior. The general chromosome morphology is very similar to Gifu, and fluorescence in situ hybridization (FISH) analysis revealed that the short arm of chromosome 1 in L. burttii is comparable to that of Gifu, indicating that the translocation event involving chromosomes 1 and 2, which was observed in L. japonicus Miyakojima MG-20, is not present in L. burttii. In addition L. burttii has a higher level of DNA polymorphism compared to Gifu and MG-20 enabling design of codominant markers such as SSR, CAPS and dCAPS. Using an F2 population from a cross between Gifu and L. burttii, codominant makers that co-segregated at the translocation site could be expanded. In order to normalize the genetic background, L. burttii was inbred for nine generations and the germplasm L. burttii B-303-S9 was established.
Kawaguchi
Masayoshi
M
Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan. masayosi@biol.s.u-tokyo.ac.jp
Pedrosa-Harand
Andrea
A
Yano
Koji
K
Hayashi
Makoto
M
Murooka
Yoshikatsu
Y
Saito
Katsuharu
K
Nagata
Toshiyuki
T
Namai
Kiyoshi
K
Nishida
Hiroshi
H
Shibata
Daisuke
D
Sato
Shusei
S
Tabata
Satoshi
S
Hayashi
Masaki
M
Harada
Kyuya
K
Sandal
Niels
N
Stougaard
Jens
J
Bachmair
Andreas
A
Grant
William F
WF
D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D000070116:Lotus / Q000145:classification / Q000235:genetics*; D008967:Molecular Biology; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D014178:Translocation, Genetic / Q000235:genetics
D018744:DNA, Plant; D005819:Genetic Markers