title: A new single nucleotide polymorphism in CAPN1 extends the current tenderness marker test to include cattle of Bos indicus, Bos taurus, and crossbred descent.
abstract: The three objectives of this study were to 1) test for the existence of beef tenderness markers in the CAPN1 gene segregating in Brahman cattle; 2) test existing CAPN1 tenderness markers in indicus-influenced crossbred cattle; and 3) produce a revised marker system for use in cattle of all subspecies backgrounds. Previously, two SNP in the CAPN1 gene have been described that could be used to guide selection in Bos taurus cattle (designated Markers 316 and 530), but neither marker segregates at high frequency in Brahman cattle. In this study, we examined three additional SNP in CAPN1 to determine whether variation in this gene could be associated with tenderness in a large, multisire American Brahman population. One marker (termed 4751) was associated with shear force on postmortem d 7 (P < 0.01), 14 (P = 0.015), and 21 (P < 0.001) in this population, demonstrating that genetic variation important for tenderness segregates in Bos indicus cattle at or near CAPN1. Marker 4751 also was associated with shear force (P < 0.01) in the same large, multisire population of cattle of strictly Bos taurus descent that was used to develop the previously reported SNP (referred to as the Germplasm Evaluation [GPE] Cycle 7 population), indicating the possibility that one marker could have wide applicability in cattle of all subspecies backgrounds. To test this hypothesis, Marker 4751 was tested in a third large, multisire cattle population of crossbred subspecies descent (including sire breeds of Brangus, Beefmaster, Bonsmara, Romosinuano, Hereford, and Angus referred to as the GPE Cycle 8 population). The highly significant association of Marker 4751 with shear force in this population (P < 0.001) confirms the usefulness of Marker 4751 in cattle of all subspecies backgrounds, including Bos taurus, Bos indicus, and crossbred descent. This wide applicability adds substantial value over previously released Markers 316 and 530. However, Marker 316, which had previously been shown to be associated with tenderness in the GPE Cycle 7 population, also was highly associated with shear force in the GPE Cycle 8 animals (P < 0.001). Thus, Marker 316 may continue to be useful in a variety of populations with a high percentage of Bos taurus backgrounds. An optimal marker strategy for CAPN1 in many cases will be to use both Markers 316 and 4751.
authors.affiliation: ARS, USDA, U.S. Meat Animal Research Center, Clay Center, NE 68933, USA.
authors.forename: S N
authors.initials: SN
authors.lastname: White
authors.forename: E
authors.initials: E
authors.lastname: Casas
authors.forename: T L
authors.initials: TL
authors.lastname: Wheeler
authors.forename: S D
authors.initials: SD
authors.lastname: Shackelford
authors.forename: M
authors.initials: M
authors.lastname: Koohmaraie
authors.forename: D G
authors.initials: DG
authors.lastname: Riley
authors.forename: C C
authors.initials: CC
authors.lastname: Chase
authors.forename: D D
authors.initials: DD
authors.lastname: Johnson
authors.forename: J W
authors.initials: JW
authors.lastname: Keele
authors.forename: T P L
authors.initials: TP
authors.lastname: Smith
mesh_terms: D000818:Animals; D002154:Calpain / Q000235:genetics* / Q000502:physiology; D002417:Cattle / Q000235:genetics* / Q000502:physiology; D003433:Crosses, Genetic; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D023061:Gene Order / Q000235:genetics; D005819:Genetic Markers / Q000502:physiology; D016678:Genome / Q000235:genetics; D005838:Genotype; D006824:Hybridization, Genetic / Q000235:genetics / Q000502:physiology; D008297:Male; D008460:Meat / Q000592:standards*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics / Q000502:physiology*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D002154:Calpain; C484807:CAPN1 protein, Bos taurus