Comparative study on the analytical performance of three waveforms for the determination of several aminoglycoside antibiotics with high performance liquid chromatography using amperometric detection.
A preliminary comparative study was carried out on the analytical performances of a new six-potential waveform and other two detection waveforms, triple-potential waveform and quadruple-potential waveform. The analytical performances compared included signal response, background noise, signal/noise ratio and signal stability. Compared with triple-potential waveform and quadruple-potential waveform, the new six-potential waveform had higher signal response, signal/noise ratio, and sensitivity. As for determination reproducibility, the six-potential waveform also exhibited a slightly better performance than the other two waveforms. Under the selected experimental conditions based on the six-potential waveform, there is a linear correlation between peak area and concentration over two to three orders of magnitude for nine aminoglycoside antibiotics with a correlation coefficients better than 0.998 and the detection limits measured as three times the peak height signal-to-noise ratio for the nine aminoglycoside antibiotics were in the range of 0.0198-0.889 microg/mL. The proposed method had been used to analyze real gentamicin sulphate drug sample.
Cai
Yaqi
Y
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
Cai
Yu'e
Y
Cheng
Jun
J
Mou
Shifen
S
Yiqiang
Lu
L
D000617:Aminoglycosides / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D005839:Gentamicins / Q000032:analysis
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D005839:Gentamicins