[Affection of different preparations on the content of toxic constituents in traditional Chinese medicines, examplified with Caulis Aristolochiae Manshuriensis].
OBJECTIVE
Taking Caulis Aristolochiae Manshuriensis (Guanmutong in Chinese, derived from the stem of Aristolochia manshuriensis) as an example, to study the affection of different preparations on the content of toxic constituents in traditional Chinese medicines.


METHODS
The separation was performed on a zorbax SB-C18 column with mobile phase of acetonitrile-3.7 mmol x L(-1) phosphoric acid buffer, detected at 260 nm.


RESULTS
The extraction percentage of aristolochic acids I, II and IV a in water extraction (1 h x 2) of Guanmutong were 53.4%, 75.5% and 61.9%, respectively; the remaining quantity of aristolochic acids I, II and IVa in the dregs of the decoction were 22.3%, 15.7% and 30.3%, respectively; Aristolochic acid I was still main substance among these aristolohic acids in the decoction of Guanmutong.


CONCLUSIONS
The content of toxic constituents of the traditional Chinese medicines varies evidently with different preparations of Guanmutong. So the preparation methods of traditional Chinese medicines should be suitably selected according to characteristics of the toxic constituents so as to lessen the body damages of human.
Zhang
Cui-Ying
CY
School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Wang
Xuan
X
Shang
Ming-Ying
MY
Yu
Jie
J
Xu
Jun-Yu
JY
Lh
Zhen-Guo
ZG
Li
Xiao-Mei
XM
Cai
Shao-Qing
SQ
Namba
Tsuneo
T
D030022:Aristolochia / Q000737:chemistry*; D034341:Aristolochic Acids / Q000032:analysis*; D004350:Drug Residues / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry* / Q000302:isolation & purification; D019999:Pharmaceutical Solutions / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders / Q000737:chemistry
D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal; D019999:Pharmaceutical Solutions; D011208:Powders