title: A sericin-derived peptide protects sf9 insect cells from death caused by acute serum deprivation.
abstract: Sericin is the silk protein enveloping fibroin fibers in cocoons. Sericin hydrolysate protects cultured Sf9 insect cells from death caused by serum deprivation; the activity depends on the repeats of 38 amino acids. A partial peptide from the 38 residues, SGGSSTYGYS, inhibited serum-deprivation death as well. Cell viabilities in the presence of 10% (v/v) foetal calf serum, no additives and 1 mM: SGGSSTYGYS were 96, 12 and 31% on the third day after inoculation, respectively. Aromatic residues seemed to be important because SGGSSTWGWS had the same activity as SGGSSTYGYS but SGGSSTAGAS had no activity.
authors.affiliation: Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjojima, 910-1195 Fukui, Japan. mastak@fpu.ac.jp
authors.forename: Masakazu
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Tsujimoto
authors.forename: Youichi
authors.initials: Y
authors.lastname: Kato
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Yamada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takagi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Nakamori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology*; D047030:Sericins / Q000737:chemistry / Q000494:pharmacology*; D018411:Spodoptera / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
chemical_list: D016895:Culture Media, Serum-Free; D010446:Peptide Fragments; D047030:Sericins