title: Study of adhesion and survival of lactobacilli and bifidobacteria on table olives with the aim of formulating a new probiotic food.
abstract: With the aim of developing new functional foods, a traditional product, the table olive, was used as a vehicle for incorporating probiotic bacterial species. Survival on table olives of Lactobacillus rhamnosus (three strains), Lactobacillus paracasei (two strains), Bifidobacterium bifidum (one strain), and Bifidobacterium longum (one strain) at room temperature was investigated. The results obtained using a selected olive sample demonstrated that bifidobacteria and one strain of L. rhamnosus (Lactobacillus GG) showed a good survival rate, with a recovery of about 10(6) CFU g(-1) after 30 days. The Lactobacillus GG population remained unvaried until the end of the experiment, while a slight decline (to about 10(5) CFU g(-1)) was observed for bifidobacteria. High viability, with more than 10(7) CFU g(-1), was observed throughout the 3-month experiment for L. paracasei IMPC2.1. This strain, selected for its potential probiotic characteristics and for its lengthy survival on olives, was used to validate table olives as a carrier for transporting bacterial cells into the human gastrointestinal tract. L. paracasei IMPC2.1 was recovered from fecal samples in four out of five volunteers fed 10 to 15 olives per day carrying about 10(9) to 10(10) viable cells for 10 days.
authors.affiliation: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via Amendola, 122/O, 70126 Bari, Italy. paola.lavermicocca@ispa.cnr.it
authors.forename: Paola
authors.initials: P
authors.lastname: Lavermicocca
authors.forename: Francesca
authors.initials: F
authors.lastname: Valerio
authors.forename: Stella Lisa
authors.initials: SL
authors.lastname: Lonigro
authors.forename: Maria
authors.initials: M
authors.lastname: De Angelis
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Morelli
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Callegari
authors.forename: Carlo G
authors.initials: CG
authors.lastname: Rizzello
authors.forename: Angelo
authors.initials: A
authors.lastname: Visconti
mesh_terms: D000328:Adult; D001422:Bacterial Adhesion; D001644:Bifidobacterium / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure; D015169:Colony Count, Microbial; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D031658:Olea / Q000382:microbiology* / Q000648:ultrastructure; D019936:Probiotics