Molecular characterization of Egyptian Trichomonas vaginalis clinical isolates by HSP70 restriction fragment length polymorphism.
Molecular typing of 20 Egyptian Trichomonas vaginalis clinical isolates was performed using the Restriction Fragment Length Polymorphism (RFLP) analysis employing a probe from the heat-inducible cytoplasmic HSP70 gene family hybridized with EcoR l-digested genomic DNA. In each of the isolates tested 5 to 6 distinct DNA fragments ranging from 2.7 Kb to 7.5 Kb in size were detected. Analysis of 13 isolates from symptomatic and 7 isolates from asymptomatic women revealed 6 distinct RFLP pattern subtypes of T. vaginalis. Eleven isolates (55%) showed the same RFLP pattern, teen of them (90.9%) were from symptomatic patients. T. vaginalis virus (TVV) was present in 7 isolates (35%). Only one isolate was considered resistant to Metronidazole. There were no relations between TVV infection or Metronidazole susceptibility and RFLP subtypes.
Hussien
Eman M
EM
Department of Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
El-Sayed
Hanan Z
HZ
El-Moamly
Amal A
AA
Helmy
Moshira M
MM
Shaban
Mohamed M
MM
D000818:Animals; D000994:Antitrichomonal Agents / Q000494:pharmacology; D016054:DNA, Protozoan / Q000737:chemistry*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology; D008970:Molecular Weight; D012150:Polymorphism, Restriction Fragment Length; D014247:Trichomonas Vaginitis / Q000469:parasitology*; D014246:Trichomonas vaginalis / Q000737:chemistry / Q000145:classification* / Q000235:genetics
D000994:Antitrichomonal Agents; D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D008795:Metronidazole