[Research of the contents of in vitro protein in the seed of sck transgenic rice].
OBJECTIVE
To detect the expression level of CpTI and HPT proteins in the seed of sck transgenic rice.


METHODS
The seeds of T6 sck transgenic rice were detected by PCR. The CpTI and HPT proteins in the seeds, stems, roots and leaves of positive plants were detected by Western blot. The CpTI and HPT proteins in the seeds of positive plants and the control plants were detected by double sandwich ELISA.


RESULTS
hpt and sck genes were found in T6 sck rice. The CpTI and HPT proteins were positive in the leaves, stems, and roots in Western blot analysis, and negative in the seeds. The content of CpTI protein is lower than LLD of double sandwich ELISA (< 14 ug/L), and HPT protein could not be detected.


CONCLUSIONS
The content of CpTI protein in the seeds of T6 sck rice is lower than LLD of double sandwich ELISA, and HPT protein could not be detected.
Wang
Rui
R
National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Chen
Song-Biao
SB
Gong
Wan-Kui
WK
Chen
Xiao-Ping
XP
Yang
Lichen
L
Zhu
Zhen
Z
Yang
Xiaoguang
X
D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis*; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics; D012639:Seeds / Q000737:chemistry* / Q000235:genetics; D014361:Trypsin Inhibitors / Q000032:analysis
D010940:Plant Proteins; D014361:Trypsin Inhibitors; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase