The secondary cell wall polymer of Geobacillus tepidamans GS5-97T: structure of different glycoforms.
Nuclear magnetic resonance spectroscopic studies of the strain-specific secondary cell wall polymer (SCWP) of the Gram-positive, moderately thermophilic organism Geobacillus tepidamans GS5-97T reveal two glycoforms consisting of identical tetrasaccharide repeating units with different chemical modifications of the amide moieties. On the basis of sugar analyses along with 1D and 2D 1H, 13C, 15N, and 31P NMR spectroscopy at natural isotope abundance, the basic backbone structure of the SCWP was established to be [beta-D-Manp-2,3-diNAcANH2-(1-->6)-alpha-D-Glcp-(1-->4)-beta-D-Manp-2,3-diNAcANH2-(1-->3)-alpha-D-GlcpNAc-(1-->]6-(1-->O)-PO2-(O-->6)-MurNAc-, with modifications of the amide groups. In one glycoform, all beta-D-Manp-2,3-diNAcANH2 (2,3-diacetamido-2,3-dideoxy-beta-D-mannopyranuronamide, ManpANH2) residues are substituted with two acetyl groups (glycoform I) at the amide group at C-6; in the other glycoform (glycoform II), only one proton of this amide group is substituted by an acetyl group. The ratio between both the glycoforms approximates 1:1.
Steindl
Christian
C
Institut für Organische Chemie, Johannes-Kepler-Universität Linz, Altenbergerstr. 69, A-4040 Linz, Austria.
Schäffer
Christina
C
Smrecki
Vilko
V
Messner
Paul
P
Müller
Norbert
N
D001406:Bacillaceae / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D011108:Polymers / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D008562:Membrane Glycoproteins; D011108:Polymers; D011135:Polysaccharides, Bacterial; C055764:S-layer proteins