title: Platelet release of beta-thromboglobulin and platelet factor 4 and serotonin in plasma samples.
abstract: OBJECTIVE
  Platelet release of alpha granule-derived CXC chemokines and dense granule-derived serotonin in plasma samples was evaluated.
  
  
  METHODS
  Concentrations of the CXC chemokines beta-TG and PF4 were assayed by an enzyme immunoassay; serotonin was measured by an HPLC method.
  
  
  RESULTS
  Beta-TG and PF4 were more easily released than serotonin by in vitro procedures. Use of the anti-platelet cocktail CTAD and preservation of the samples at 4 degrees C were necessary to accurately measure beta-TG and PF4, but not serotonin.
  
  
  CONCLUSIONS
  Assaying serotonin may be useful for assessing platelet activation in vivo as a laboratory test because of facile preparation of plasma samples.
authors.affiliation: Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.
authors.forename: Ryunosuke
authors.initials: R
authors.lastname: Ohkawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hirowatari
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ohkubo
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ikeda
authors.forename: Mitsumasa
authors.initials: M
authors.lastname: Okada
authors.forename: Minoru
authors.initials: M
authors.lastname: Tozuka
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakahara
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yatomi
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000328:Adult; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D019743:Chemokines, CXC / Q000097:blood / Q000378:metabolism*; D019343:Citric Acid / Q000494:pharmacology; D004176:Dipyridamole / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D015539:Platelet Activation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D010978:Platelet Factor 4 / Q000378:metabolism*; D012701:Serotonin / Q000097:blood / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology; D001620:beta-Thromboglobulin / Q000378:metabolism*
chemical_list: D019743:Chemokines, CXC; D010975:Platelet Aggregation Inhibitors; D001620:beta-Thromboglobulin; D019343:Citric Acid; D012701:Serotonin; D010978:Platelet Factor 4; D004176:Dipyridamole; D004492:Edetic Acid; D013806:Theophylline; D000241:Adenosine