Differential identification of mycoplasma pulmonis and M. arthritidis using PCR-based RFLP.
Mycoplasma pulmonis and Mycoplasma arthritidis were differentially identified using PCR-restriction fragment length polymorphism (RFLP). A genus-specific sequence of mycoplasma was amplified by PCR and the PCR products were digested with the restriction enzyme SmaI. Each PCR product from the four isolates of M. pulmonis was digested with SmaI into two fragments; however, there was no digestion in the PCR product from M. arthritidis. This method might be useful to differentiate infection of M. pulmonis from that of M. arthritidis.
Kim
Dong-Jae
DJ
Department of Laboratory Animal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Park
Jong-Hwan
JH
Seok
Seung-Hyeok
SH
Cho
Sun-A
SA
Baek
Min-Won
MW
Lee
Hui-Young
HY
Yang
Ki-Hwa
KH
Jang
Dong Deuk
DD
Han
Beom-Seok
BS
Park
Jae-Hak
JH
D000818:Animals; D001170:Arthritis, Infectious / Q000175:diagnosis / Q000662:veterinary*; D004269:DNA, Bacterial / Q000032:analysis; D003937:Diagnosis, Differential; D051379:Mice; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D045645:Mycoplasma arthritidis / Q000235:genetics / Q000302:isolation & purification*; D045803:Mycoplasma pulmonis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D012376:Rodent Diseases / Q000175:diagnosis*
D004269:DNA, Bacterial