Time-resolved fluorescent chirality sensing and imaging of malate in aqueous solution.
Chiral discrimination of malates in aqueous solutions at near-neutral pH is achieved through fluorescence measurement and imaging using the europium-tetracycline complex (EuTc) as a fluorescent probe. The method is based on the significantly different fluorescence properties of the ternary complexes (Eu-Tc-malate) formed between EuTc and the enantiomeric malates. The enantiomeric excess (ee) of chiral malates can be quantified by both steady-state and time-resolved fluorescence, using either a conventional fluorescence microplate reader or fluorescence imaging. It offers a facile and sensitive method for high-throughput chiral discrimination.
Lin
Zhihong
Z
Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany.
Wu
Meng
M
Wolfbeis
Otto S
OS
D005063:Europium; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008293:Malates / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013995:Time; D014867:Water / Q000737:chemistry*
D005456:Fluorescent Dyes; D008293:Malates; D014867:Water; D005063:Europium; C030298:malic acid