[Red/ET recombination and its biomedical applications].
Red/ET recombination, a powerful homologous recombination system based on the Red operon of lambda phage or RecE/ RecT from Rac phage, provides an innovative approach for DNA engineering. Deletion, insertion and mutation can be quickly and precisely performed on the target gene mediated by Red/ET recombination with PCR derived DNA fragments or oligonucleotides. This technical platform has extensive applications in biomedical field including bacterial artificial chromosome modification, gene knock-out construction and genetic modification of E. coli strains as well as some other kinds of microorganisms. Recently, Red/ET recombination was improved in several aspects so that it becomes more powerful and maneuverable. The characteristic and development of Red/ET recombination and its biomedical applications were described in this review.
Wang
Jun-Ping
JP
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, The Third Military Medical University, Chongqing 400038, China. wangjunp@yahoo.com
Zhang
You-Ming
YM
D010582:Bacteriophage lambda / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D011938:Rec A Recombinases / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics*
D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C084497:RecT protein, E coli; D011938:Rec A Recombinases; D005090:Exodeoxyribonucleases; C038225:recE protein, E coli