Disruption of histone deacetylase gene RPD3 accelerates PHO5 activation kinetics through inappropriate Pho84p recycling.
The histone deacetylase Rpd3p functions as a transcriptional repressor of a diverse set of genes, including PHO5. Here we describe a novel role for RPD3 in the regulation of phosphate transporter Pho84p retention in the cytoplasmic membrane. We show that under repressing conditions (with P(i)), PHO5 expression is increased in a pho4Delta rpd3Delta strain, demonstrating PHO regulatory pathway independence. However, the effect of RPD3 disruption on PHO5 activation kinetics is dependent on the PHO regulatory pathway. Upon switching to activating conditions (without P(i)), PHO5 transcripts accumulated more rapidly in rpd3Delta cells. This more rapid response correlates with a defect in phosphate uptake due to premature recycling of Pho84p, the high-affinity H+/PO4(3-) symporter. Thus, RPD3 also participates in PHO5 regulation through a previously unidentified effect on maintenance of high-affinity phosphate uptake during phosphate starvation. We propose that Rpd3p has a negative role in the regulation of Pho84p endocytosis.
Wongwisansri
Sriwan
S
Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1870, USA.
Laybourn
Paul J
PJ
D000135:Acid Phosphatase / Q000378:metabolism; D001692:Biological Transport; D002462:Cell Membrane / Q000201:enzymology; D004705:Endocytosis; D015966:Gene Expression Regulation, Fungal; D005838:Genotype; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D028061:Phosphate Transport Proteins / Q000235:genetics / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D028081:Proton-Phosphate Symporters / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D048348:Reverse Transcription; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction; D013489:Suppression, Genetic; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
C105495:PHO84 protein, S cerevisiae; D028061:Phosphate Transport Proteins; D010710:Phosphates; D028081:Proton-Phosphate Symporters; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000135:Acid Phosphatase; C488916:PHO5 protein, S cerevisiae; D006655:Histone Deacetylases