title: Coxiella burnetii genotyping.
abstract: Coxiella burnetii is a strict intracellular bacterium with potential as a bioterrorism agent. To characterize different isolates of C. burnetii at the molecular level, we performed multispacer sequence typing (MST). MST is based on intergenic region sequencing. These regions are potentially variable since they are subject to lower selection pressure than the adjacent genes. We screened 68 spacers in 14 isolates and selected the 10 that exhibited the most variation. These spacers were then tested in 159 additional isolates obtained from different geographic areas or different hosts or were implicated in different manifestations of human disease caused by C. burnetii. The sequence analysis yielded 30 different allelic combinations. Phylogenic analysis showed 3 major clusters. MST allows easy comparison and exchange of results obtained in different laboratories and could be a useful tool for identifying bacterial strains.
authors.affiliation: Unité des Rickettsies, Faculté de Médecine, CNRS UMR 6020, IFR48, Marseille, France.
authors.forename: Olga
authors.initials: O
authors.lastname: Glazunova
authors.forename: Véronique
authors.initials: V
authors.lastname: Roux
authors.forename: Olga
authors.initials: O
authors.lastname: Freylikman
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Sekeyova
authors.forename: Ghislain
authors.initials: G
authors.lastname: Fournous
authors.forename: Judith
authors.initials: J
authors.lastname: Tyczka
authors.forename: Nikolai
authors.initials: N
authors.lastname: Tokarevich
authors.forename: Elena
authors.initials: E
authors.lastname: Kovacava
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Marrie
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
mesh_terms: D001483:Base Sequence; D016000:Cluster Analysis; D016997:Coxiella burnetii / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D010802:Phylogeny; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011778:Q Fever / Q000382:microbiology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer