Dendritic-NK cell interactions in P. gingivalis-specific responses.
Patients with localized aggressive periodontitis have type-1 cytokines in gingival crevicular fluid and high titers of IFN-gamma-dependent IgG2 reactive with P. gingivalis in gingival crevicular fluid and serum. Localized aggressive periodontitis monocytes spontaneously differentiate into dendritic cells that can stimulate IFN-gamma production by NK cells. These relationships prompted the hypothesis that P. gingivalis-dendritic cell-NK cell interactions might promote type-1 cytokine responses. Although P. gingivalis is not a potent inducer of Th1 responses, it stimulated strong IL-12 responses by monocyte-derived dendritic cells in the presence of IFN-gamma, and IFN-gamma was produced by NK cells within 24 hrs in the presence of dendritic cells. Anti-P. gingivalis IgG2 responses were enhanced by dendritic cells, and removal of NK cells reduced IFN-gamma- and P. gingivalis-specific IgG2. Thus, P. gingivalis-dendritic cell-NK cell interactions apparently resulted in reciprocal stimulation and increased type-1 cytokine production by both dendritic cells and NK cells, and increased P. gingivalis-specific IgG2.
Kikuchi
T
T
Clinical Research Center for Periodontal Diseases, School of Dentistry, VCU, PO Box 980556, Richmond, VA 23298-0556, USA.
Willis
D L
DL
Liu
M
M
Purkall
D B
DB
Sukumar
S
S
Barbour
S E
SE
Schenkein
H A
HA
Tew
J G
JG
D000328:Adult; D000704:Analysis of Variance; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000378:metabolism; D002450:Cell Communication; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism / Q000382:microbiology / Q000502:physiology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000378:metabolism / Q000382:microbiology / Q000502:physiology*; D051379:Mice; D009504:Neutrophils / Q000378:metabolism; D010518:Periodontitis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma