Effect of molecular weight heterogeneity on drug encapsulation efficiency of gelatin nano-particles.
Influence of molecular weight heterogeneity and drug solubility, drug loading and hydrodynamic conditions on drug release kinetics from gelatin nanoparticles were investigated. Also to assess the ability of gelatin nanoparticles as a potential intravascular probe for diagnostic purposes and in improving the biodelivery of cycloheximide (CHX), which is being used as a representative drug. Comparative characterization of 75 Bloom (type B, bovine), 175 and 300 Bloom (type A, porcine) gelatin nanoparticles was done to understand the phase behavior and hydrodynamic properties of gelatin chains and its nanoparticles. Gelatin nanoparticles were prepared by two-step desolvation method. Dynamic light scattering studies were performed to estimate hydrodynamic radii as well as intermolecular interaction. Effects of parameters like pH, temperature and molecular weight on the size and stability of the nanoparticles were studied. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) measurements were done for size and stability analysis. Enhanced visco-elastic properties of nanoparticles were observed as compared to normal solutions of gelatin.
Saxena
Anita
A
School of Physical Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Sachin
Kumar
K
Bohidar
H B
HB
Verma
Anita Kamra
AK
D005780:Gelatin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D036103:Nanotechnology; D010316:Particle Size; D004364:Pharmaceutical Preparations; D013696:Temperature; D014783:Viscosity
D004364:Pharmaceutical Preparations; D005780:Gelatin