Polymorphisms of CD14 gene and TLR4 gene are not associated with ulcerative colitis in Chinese patients.
OBJECTIVE
Toll-like receptor 4 and CD14 are the components of the lipopolysaccharide receptor complex. The aim of this study was to investigate the associations between polymorphisms TLR4 Asp299Gly and CD14 C-260T and Chinese patients with ulcerative colitis (UC).


METHODS
Using a polymerase chain reaction based restriction fragment length polymorphism, the study genotyped polymorphisms TLR4 Asp299Gly and CD14 C-260T in 114 patients with UC and 160 healthy controls in the Chinese Han population. Moreover a comparison was made with 170 healthy Dutch white subjects.


RESULTS
No TLR4 Asp299Gly mutation was detected in any patients or healthy controls in the Chinese Han population, which was similar to Japanese subjects, but the mutation occurred in 10% of the Dutch white subjects. There were no significant differences of CD14 genotypes between healthy controls and the patients with UC.
Guo
Q S
QS
Department of Internal Medicine and Geriatrics, Research Centre of Digestive Diseases, Zhongnan Hospital, Wuhan University Medical School, Wuhan, PR of China.
Xia
B
B
Jiang
Y
Y
Morré
S A
SA
Cheng
L
L
Li
J
J
Crusius
J B A
JB
Peña
A S
AS
D044466:Asian People; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000208:ethnology / Q000235:genetics*; D016001:Confidence Intervals; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008297:Male; D016017:Odds Ratio; D011110:Polymorphism, Genetic / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics*
D018950:Lipopolysaccharide Receptors; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4