Layer by layer self-assembled polyelectrolyte multilayers with embedded phospholipid vesicles obtained by spraying: integrity of the vesicles.
In a previous paper (Michel, M.; Vautier, D.; Voegel, J.-C.; Schaaf, P.; Ball, V. Langmuir 2004, 20, 4835), we showed that phospholipid vesicles can be incorporated into poly(glutamic-acid)/poly(allylamine) (PGA/PAH) multilayered polyelectrolyte films built by the alternated dipping of a surface in polyanion and polycation solutions. AFM imaging, quartz crystal microbalance, and ellipsometry suggested that the vesicles remain intact when adhering on the surface. In the present paper, we show that such films can also be realized by spraying both the polyelectrolyte solutions and the vesicles onto the surface. Using such vesicles filled with ferrocyanide ions, we prove by cyclic voltammetry that the sprayed vesicles remain intact when embedded in the multilayers. We show that multilayers containing two distinct layers of intact vesicles separated by several polyanion/polycation bilayers can also be constructed. Polyelectrolyte multilayers containing layers of phospholipid vesicles could act as reservoirs for drug or other biologically active molecules in controlled release bioactive coatings.
Michel
M
M
Institut National de la Santé et de la Recherche Médicale, Unité 595, Faculté de Médecine, 11 rue Humann, 67085 Strasbourg Cedex, France.
Izquierdo
A
A
Decher
G
G
Voegel
J-C
JC
Schaaf
P
P
Ball
V
V
D004563:Electrochemistry; D004573:Electrolytes / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry; D008567:Membranes, Artificial; D010743:Phospholipids / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D011099:Polyglutamic Acid / Q000737:chemistry*; D013499:Surface Properties
D004573:Electrolytes; D005295:Ferrocyanides; D008567:Membranes, Artificial; D010743:Phospholipids; D011073:Polyamines; D011099:Polyglutamic Acid; C020354:hexacyanoferrate II