Differential diagnosis between complete and partial mole by TSSC3 antibody completely correlates to DNA diagnosis.
Complete hydatidiform moles (CHMs) are a type of androgenetic fertilization without an ovum. Cases of CHM exhibit a generalized swelling of the villi and are known to be highly associated with persistent disease or carcinoma. In contrast, partial hydatidiform moles (PHMs) also show characteristic hydropic changes among the villi, but the incidence of secondary disease is relatively low. Because PHMs are fertilized by one ovum and two sperm and CHMs are fertilized by one or two sperm alone, we considered whether or not maternally imprinted genes might be useful for achieving a differential diagnosis. The validity of the imprinted genes in CHMs was assessed by implementation of a microarray technique. Among the genes examined, TSSC3, SLC22A1L, KCNQ1, and Decorin were shown to be down-regulated, and TSSC3 was the most markedly suppressed of these genes. In this study, 20 cases of CHM, the diagnosis of which was confirmed by DNA polymorphism, were investigated. In all of these cases, the expression of TSSC3 was completely absent, as determined by Western blot analysis. Conversely, 12 cases of PHM, also diagnosed by DNA polymorphism, were examined here; in all of these 12 cases, TSSC3 was found to be expressed normally. Immunohistochemical (IHC) analysis also produced the same results. The complete silencing of TSSC3 in cases of CHM will provide a novel, convenient strategy for the diagnosis of molar lesions in the placenta.
Kato
Hidenori
H
Division of Molecular and Cell Therapeutics, Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, Oita, Japan. hidekato@beppu.kyushu-u.ac.jp
Matsuda
Takao
T
Hirakawa
Toshio
T
Ueda
Kyoko
K
Inoue
Takafumi
T
Miyanari
Yoko
Y
Asanoma
Kazuo
K
Nakano
Hitoo
H
Wake
Norio
N
D000906:Antibodies; D015153:Blotting, Western; D058575:Decorin; D003937:Diagnosis, Differential; D016326:Extracellular Matrix Proteins; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D006828:Hydatidiform Mole / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D027701:Organic Cation Transport Proteins / Q000235:genetics; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics; D011247:Pregnancy; D011248:Pregnancy Complications / Q000175:diagnosis; D011509:Proteoglycans / Q000235:genetics
D000906:Antibodies; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; C485621:KCNQ1OT1 long non-coding RNA, human; D008565:Membrane Proteins; D009687:Nuclear Proteins; D027701:Organic Cation Transport Proteins; D024642:Potassium Channels, Voltage-Gated; D011509:Proteoglycans; C111653:SLC22A18 protein, human; C109277:TSSC3 protein