title: Conjugation of multiple copies of polyethylene glycol to hemoglobin facilitated through thiolation: influence on hemoglobin structure and function.
abstract: PEGylation induced changes in molecular volume and solution properties of HbA have been implicated as potential modulators of its vasoconstrictive activity. However, our recent studies with PEGylated Hbs carrying two PEG chains/Hb, have demonstrated that the modulation of the vasoconstrictive activity of Hb is not a direct correlate of the molecular volume and solution properties of the PEGylated Hb and implicated a role for the surface charge and/or the pattern of surface decoration of Hb with PEG. HbA has now been modified by thiolation mediated maleimide chemistry based PEGylation that does not alter its surface charge and conjugates multiple copies of PEG5K chains. This protocol has been optimized to generate a PEGylated Hb, (SP-PEG5K)(6)-Hb, that carries approximately six PEG5K chains/Hb - HexaPEGylated Hb. PEGylation increased the O(2) affinity of Hb and desensitized the molecule for the influence of ionic strength, pH, and allosteric effectors, presumably a consequence of the hydrated PEG-shell generated around the protein. The total PEG mass in (SP-PEG5K)(6)-Hb, its molecular volume, O(2) affinity and solution properties are similar to that of another PEGylated Hb, (SP-PEG20K)(2)-Hb, that carries two PEG20K chains/Hb. However, (SP-PEG5K)(6)-Hb exhibited significantly reduced vasoconstriction mediated response than (SP-PEG20K)(2)-Hb. These results demonstrate that the enhanced molecular size and solution properties achieved through the conjugation of multiple copies of small PEG chains to Hb is more effective in decreasing its vasoconstrictive activity than that achieved through the conjugation of a comparable PEG mass using a small number of large PEG chains.
authors.affiliation: Departments of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Belur N
authors.initials: BN
authors.lastname: Manjula
authors.forename: Amy G
authors.initials: AG
authors.lastname: Tsai
authors.forename: Marcos
authors.initials: M
authors.lastname: Intaglietta
authors.forename: Ching-Hsuan
authors.initials: CH
authors.lastname: Tsai
authors.forename: Chien
authors.initials: C
authors.lastname: Ho
authors.forename: Paul K
authors.initials: PK
authors.lastname: Smith
authors.forename: Krishnaveni
authors.initials: K
authors.lastname: Perumalsamy
authors.forename: Nirmala Devi
authors.initials: ND
authors.lastname: Kanika
authors.forename: Joel M
authors.initials: JM
authors.lastname: Friedman
authors.forename: Seetharama A
authors.initials: SA
authors.lastname: Acharya
mesh_terms: D000818:Animals; D006224:Cricetinae; D006439:Hemodynamics; D006441:Hemoglobin A / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008301:Maleimides / Q000737:chemistry; D015394:Molecular Structure; D009997:Osmotic Pressure; D011092:Polyethylene Glycols / Q000737:chemistry*; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D014783:Viscosity
chemical_list: D008301:Maleimides; D021122:Protein Subunits; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols; D006441:Hemoglobin A