title: Leukotoxin family genes in Staphylococcus aureus isolated from domestic animals and prevalence of lukM-lukF-PV genes by bacteriophages in bovine isolates.
abstract: Leukotoxin family genes in Staphylococcus aureus isolated from domestic animals were examined by polymerase chain reaction. LukS and lukF genes were detected in all 48 avian and 72 porcine isolates of S. aureus. LukE and lukD genes, located in a putative staphylococcal pathogenicity island (Sapln3/Saplm3), were recognized in 44 (91.7%) of 48 avian isolates, but these genes were not detected in porcine isolates. In 297 bovine isolates collected from mastitic cow's milk and bulk milk from dairy farms in two regions, lukM and lukF-PV(P83) genes in addition to lukS-lukF and lukE-lukD genes were detected in 100 (62.5%) of the 160 isolates from Ishikawa and in118 (86.1%) of the 137 isolates from Hokkaido. When the lysogeny of S. aureus bovine isolates was examined by treatment with mitomycin C, clearing of the culture due to cell lysis was observed in 34 (91.9%) of 37 lukM-lukF-PV(P83) genes--positive isolates. In addition, we isolated a novel lukM-lukF-PV(P83)-carrying (designated phiLukM), and revealed that the lukM-lukF-PV(P83) genes were located very close to an amidase gene on the temperate phage genomes. These results suggest horizontal transmission of lukM-lukF-PV(P83) genes by temperate bacteriophages in S. aureus of bovine origin.
authors.affiliation: Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University, 4-1-1 Kenjyojima Matsuoka, Fukui 910-1195, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yamada
authors.forename: Naoko
authors.initials: N
authors.lastname: Tochimaru
authors.forename: Sachiko
authors.initials: S
authors.lastname: Nakasuji
authors.forename: Eiji
authors.initials: E
authors.lastname: Hata
authors.forename: Hideki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Masashi
authors.initials: M
authors.lastname: Eguchi
authors.forename: Jun
authors.initials: J
authors.lastname: Kaneko
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kamio
authors.forename: Toshio
authors.initials: T
authors.lastname: Kaidoh
authors.forename: Shotaro
authors.initials: S
authors.lastname: Takeuchi
mesh_terms: D000820:Animal Diseases / Q000382:microbiology*; D000818:Animals; D000829:Animals, Domestic; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001717:Birds; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D005098:Exotoxins / Q000096:biosynthesis / Q000235:genetics*; D007166:Immunosuppressive Agents / Q000378:metabolism; D007956:Leukocidins; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013204:Staphylococcus Phages; D013211:Staphylococcus aureus / Q000235:genetics*; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005098:Exotoxins; D007166:Immunosuppressive Agents; D007956:Leukocidins; C018579:leukotoxin; C074300:LukF protein, Staphylococcus aureus