title: A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation.
abstract: Phosphorus, one of the essential elements for plants, is often a limiting nutrient because of its low availability and mobility in soils. Significant changes in plant morphology and biochemical processes are associated with phosphate (Pi) deficiency. However, the molecular bases of these responses to Pi deficiency are not thoroughly elucidated. Therefore, a comprehensive survey of global gene expression in response to Pi deprivation was done by using Arabidopsis thaliana whole genome Affymetrix gene chip (ATH1) to quantify the spatio-temporal variations in transcript abundance of 22,810 genes. The analysis revealed a coordinated induction and suppression of 612 and 254 Pi-responsive genes, respectively. The functional classification of some of these genes indicated their involvement in various metabolic pathways, ion transport, signal transduction, transcriptional regulation, and other processes related to growth and development. This study is a detailed analysis of Pi starvation-induced changes in gene expression of the entire genome of Arabidopsis correlated with biochemical processes. The results not only enhance our knowledge about molecular processes associated with Pi deficiency, but also facilitate the identification of key molecular determinants for improving Pi use by crop species.
authors.affiliation: Laboratoire de Biologie du Développement des Plantes, Unite Mixte de Recherche 6191, Centre National de la Recherche Scientifique-Commissariat à l'Energie Atomique, Aix-Marseille II, 13108 Saint-Paul-lez-Durance, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Misson
authors.forename: Kashchandra G
authors.initials: KG
authors.lastname: Raghothama
authors.forename: Ajay
authors.initials: A
authors.lastname: Jain
authors.forename: Juliette
authors.initials: J
authors.lastname: Jouhet
authors.forename: Maryse A
authors.initials: MA
authors.lastname: Block
authors.forename: Richard
authors.initials: R
authors.lastname: Bligny
authors.forename: Philippe
authors.initials: P
authors.lastname: Ortet
authors.forename: Audrey
authors.initials: A
authors.lastname: Creff
authors.forename: Shauna
authors.initials: S
authors.lastname: Somerville
authors.forename: Norbert
authors.initials: N
authors.lastname: Rolland
authors.forename: Patrick
authors.initials: P
authors.lastname: Doumas
authors.forename: Philippe
authors.initials: P
authors.lastname: Nacry
authors.forename: Luis
authors.initials: L
authors.lastname: Herrerra-Estrella
authors.forename: Laurent
authors.initials: L
authors.lastname: Nussaume
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Thibaud
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D010710:Phosphates / Q000172:deficiency* / Q000494:pharmacology; D015203:Reproducibility of Results; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D010710:Phosphates