Modification of the content of plasma protein carbonyl groups in donors after granulocyte colony stimulating factor-induced stem cell mobilization.
Granulocyte-colony stimulating factor (G-CSF), the most established agent for the mobilization of stem cells in current clinical practice, could induce a condition of oxidative stress. Herein plasmatic levels of protein carbonyl groups (a biomarker of oxidative stress) were measured in a group of donors given recombinant human G-CSF (rHuG-CSF) at different times: (1) before starting rHuG-CSF administration, (2) on day 5 of rHuG-CSF administration, (3) on the same day immediately after the end of the first leukapheresis procedure and (4) one week after rHuG-CSF withdrawal. Plasma levels of protein carbonyl groups enhanced significantly in donors after 5 days of rHuG-CSF treatment and appeared further slightly increased following leukapheresis procedure; 7 days following rHuG-CSF withdrawal, they showed a tendency to normalization. These findings may contribute to better understand the oxidative reactions following rHuG-CSF treatment and leukapheresis.
Morabito
Fortunato
F
Bone Marrow Transplant Unit, Azienda Ospedaliera, Bianchi-Melacrino-Morelli, 89100 Reggio Calabria, Italy.
Tomaino
Antonio
A
Cristani
Mariateresa
M
Cimino
Francesco
F
Martino
Massimo
M
Minciullo
Paola Lucia
PL
Calabrò
Concetta
C
Saija
Antonella
A
Gangemi
Sebastiano
S
D000293:Adolescent; D000328:Adult; D001782:Blood Donors; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D006801:Humans; D007937:Leukapheresis / Q000379:methods; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011994:Recombinant Proteins
D001798:Blood Proteins; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor