[Cultures of transgenic crown gall from Panax quinquefolium and its total ginsenosides content].
It was clearly demonstrated that the T-DNA of Agrobacterium tumefaciens Ti plastid was integrated into the crown gall of Panax quinquefolium L. by the experiments of paper electrophoresis. The influences of cultural factors on the growth of the crown gall and the ginesnosides accumulation were reported. The results showed that MS medium was the best one for the growth of the crown gall. The growth and total ginsenoside content reached their maximum at 24th and 28th day, respectively.
Song
Yongho
Y
College of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang.
Xu
Xhenxia
X
Jin
Qianxing
Q
Yu
Rongmin
R
D016960:Agrobacterium tumefaciens / Q000235:genetics; D002244:Carbon; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D036145:Ginsenosides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D005894:Panax / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018547:Plant Stems / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development*; D013696:Temperature
D003470:Culture Media; D036145:Ginsenosides; D002244:Carbon