Neuroanatomical differences between mouse strains as shown by high-resolution 3D MRI.
The search for new mouse models of human disease requires a sensitive metric to make three-dimensional (3D) anatomical comparisons in a rapid and quantifiable manner. This is especially true in the brain, where changes in complex shapes such as the hippocampus and ventricles are difficult to assess with 2D histology. Here, we report that the 3D neuroanatomy of three strains of mice (129S1/SvImJ, C57/Bl6, and CD1) is significantly different from one another. Using image co-registration, we 'morphed' together nine brains of each strain scanned by magnetic resonance imaging at (60 microm)3 resolution to synthesize an average image. We applied three methods of comparison. First, we used visual inspection and graphically examined the standard deviation of the variability in each strain. Second, we annotated 42 neural structures and compared their volumes across the strains. Third, we assessed significant local deviations in volume and displacement between the two inbred strains, independent of prior anatomical knowledge.
Chen
X Josette
XJ
Mouse Imaging Centre, Hospital for Sick Children, Department of Medical Biophysics, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8. josette@sickkids.ca
Kovacevic
Natasa
N
Lobaugh
Nancy J
NJ
Sled
John G
JG
Henkelman
R Mark
RM
Henderson
Jeffrey T
JT
D000465:Algorithms; D000818:Animals; D001931:Brain Mapping; D007091:Image Processing, Computer-Assisted; D016018:Least-Squares Analysis; D008279:Magnetic Resonance Imaging; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains / Q000033:anatomy & histology*; D013045:Species Specificity; D009626:Terminology as Topic