title: Application of dye-enhanced laser ablation for liver resection. Production of protein sealant on the cut surface of the liver by enhanced thermal energy of low power diode laser.
abstract: BACKGROUND
  Dye-enhanced laser ablation (DLA) using a low-power diode laser for indocyanine green (ICG)-stained tissue has proven its effectiveness in dye-enhanced laser photocoagulation of retinal vessels or endoscopic surgical mucosectomy. We have applied DLA in hepatectomy and described its histological distinction in comparison with the cavitron ultrasonic surgical aspirator (CUSA).
  
  
  METHODS
  A diode laser (UDL-60 Laser unit, Olympus, Tokyo, Japan) with 810 +/- 20 nm wavelength was employed for this study. The ICG dye (Diagnogreen, Daiichi Pharmaceutical, Tokyo, Japan) with a peak absorption wavelength at 800-810 nm was injected topically into the resection plane of the liver. The liver tissue was divided by touching the tip of the diode laser. Three different concentrations of ICG solution such as 2.0, 1.0 and 0.5 mg/ml were tested in the preliminary animal experiment. The use of a low-power diode laser at 10 W with an ICG concentration of 0.5 mg/ml was the appropriate combination for liver resection. In the clinical series, 27 hepatectomies were performed by DLA, and 10 with CUSA.
  
  
  RESULTS
  DLA demonstrated smooth cutting and good hemostasis in liver resection. Among the hepatectomy cases given DLA, no postoperative hemorrhage or bile leakage was noted. The postoperative hospital stay was significantly shorter in the DLA than the CUSA group. The cut surface of the liver was sealed microscopically with a layer of protein coagulum.
  
  
  CONCLUSIONS
  A layer of protein sealant on the cut surface of the liver contributes to the short postoperative hospital stay when using DLA.
authors.affiliation: Second Department of Surgery, Fukuoka University School of Medicine, Fukuoka, Japan. noritomi@cis.fukuoka-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Noritomi
authors.forename: Y
authors.initials: Y
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Kodama
authors.forename: K
authors.initials: K
authors.lastname: Mikami
authors.forename: T
authors.initials: T
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Konno
authors.forename: T
authors.initials: T
authors.lastname: Maekawa
authors.forename: T
authors.initials: T
authors.lastname: Shirakusa
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D004396:Coloring Agents; D004285:Dogs; D005260:Female; D006488:Hemostasis, Surgical / Q000379:methods*; D006498:Hepatectomy / Q000379:methods*; D006801:Humans; D007208:Indocyanine Green; D053685:Laser Therapy / Q000379:methods*; D007902:Length of Stay; D008099:Liver / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D013396:Suction; D014465:Ultrasonics
chemical_list: D004396:Coloring Agents; D007208:Indocyanine Green