Nutrient absorption by Aphidius ervi larvae.
It is well documented that in the model system Aphidius ervi Haliday (Hymenoptera, Braconidae)/Acyrthosiphon pisum (Harris) (Homoptera, Aphididae) host regulation by the parasitoid larva induces in the aphid haemolymph major changes of the titer of nutritional compounds such as proteins, acylglycerols and free amino acids, in order to meet the stage-specific demands of the developing larva. Since little is known about how the larva absorbs these mobilized nutritional resources, nutrient absorption by larval stages of A. ervi was studied. In 2nd instar larvae, leucine was ten-fold accumulated in the haemocoel, and tyrosine and glutamine two-fold. Glucose and fructose were readily absorbed and fructose was extensively metabolized by larval tissues. In 3rd instars, the presence of a number of larvae that did not ingest the incubation medium enabled us to determine the respective amounts of substrate absorbed by the epidermis and the midgut. An accumulation of leucine in the haemocoel was observed only when midgut cells were involved in absorption, while the amino acid concentration within body fluids never exceeded that of the incubation medium when the uptake was performed only by epidermal cells. The immunofluorescence analysis, the mutual inhibition exerted on labeled glucose or fructose uptakes by a 100-fold excess of the sugars and the strong inhibition of uptakes induced by 0.2mM cytochalasin B support the expression of facilitative GLUT2-like transporters in the apical and basal cell membranes of midgut epithelial cells. Taken together, these results prove that both midgut and epidermis are involved in nutrient absorption throughout the parasitoid development, that GLUT2 transporters are responsible for glucose and fructose uptakes and that the chemical gradient that favors the passive influx of the two sugars is maintained by their conversion to other substrates.
Caccia
S
S
Dipartimento di Biologia, Università di Milano, via Celoria 26, 20133 Milano, Italy.
Leonardi
M G
MG
Casartelli
M
M
Grimaldi
A
A
de Eguileor
M
M
Pennacchio
F
F
Giordana
B
B
D000042:Absorption; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D004044:Dietary Proteins / Q000378:metabolism*; D004064:Digestive System / Q000254:growth & development / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism; D005260:Female; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D051273:Glucose Transporter Type 2 / Q000378:metabolism; D005989:Glycerides / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D023421:Models, Animal; D013379:Substrate Specificity; D014443:Tyrosine / Q000378:metabolism; D014863:Wasps / Q000254:growth & development / Q000378:metabolism*
D000596:Amino Acids; D004044:Dietary Proteins; D051273:Glucose Transporter Type 2; D005989:Glycerides; D019476:Insect Proteins; D005632:Fructose; D014443:Tyrosine; D007930:Leucine; D005947:Glucose