title: Transcriptome overview of Paracoccidioides brasiliensis proteases.
abstract: Proteases perform a wide variety of functions inside and outside cells, regulating many biological processes. Infectious microorganisms use proteases, either secreted or attached to their cell surface to weaken and invade their hosts. Therefore, proteases are targets for drugs against a diverse set of diseases. Paracoccidioides brasiliensis is the most prevalent fungal pathogen causing systemic mycosis in Latin America. The development of paracoccidioidomycosis depends on interactions between fungal and host components and proteases have been described as important factors implicated in the mechanism of host colonization by fungi. The primary goal for this study is to present an overview of the transcriptome sequences--identified cDNAs that encode proteases. We obtained a total of 53 cDNAs encoding proteases; 15 were classified as ATP-independent, 12 as ATP-dependent, 22 as proteasome subunits, and 4 as deubiquitinating proteases. The mechanisms and biological activity of these proteases differ in substrate specificity and in catalytic mechanisms.
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001-970 Goiânia, GO, Brazil.
authors.forename: Juliana Alves
authors.initials: JA
authors.lastname: Parente
authors.forename: Milce
authors.initials: M
authors.lastname: Costa
authors.forename: Maristela
authors.initials: M
authors.lastname: Pereira
authors.forename: Célia Maria de Almeida
authors.initials: CM
authors.lastname: Soares
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics; D020224:Expressed Sequence Tags; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010228:Paracoccidioides / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D010229:Paracoccidioidomycosis / Q000821:virology; D010447:Peptide Hydrolases / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*; D014774:Virulence
chemical_list: D018076:DNA, Complementary; D010447:Peptide Hydrolases