title: In vivo reporter labeling of proteins via metabolic delivery of coenzyme A analogues.
abstract: We demonstrate site-specific reporter labeling of proteins within live cells. By accessing the coenzyme A (CoA) metabolic pathway with a cell-permeable pantetheine analogue, we deliver CoA-bound reporter molecules for post-translational protein modification in vivo. These methods may be applied to natural product pathway manipulation as well as applications in conventional molecular and cellular biology.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0358, USA.
authors.forename: Kristine M
authors.initials: KM
authors.lastname: Clarke
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Mercer
authors.forename: James J
authors.initials: JJ
authors.lastname: La Clair
authors.forename: Michael D
authors.initials: MD
authors.lastname: Burkart
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D003065:Coenzyme A / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D010204:Pantetheine / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D005456:Fluorescent Dyes; D010204:Pantetheine; D003065:Coenzyme A