Expression of HSG is essential for mouse blastocyst formation.
It has been shown recently that hyperplasia suppressor gene (HSG) is a powerful regulator for cell proliferation and has a critical role in mitochondrial fusion in many cells. However, little is known about its expression, localization, and function during oocyte maturation and early embryogenesis. In this study, with indirect immunofluorescent staining and Western blotting, we found that HSG was expressed in mouse oocytes and preimplantation embryos which primarily exhibited a submembrane distribution pattern in the cytoplasm. Moreover, HSG mainly associated with beta-tubulin during oocyte maturation and early embryonic development. When mouse zygotes were injected with HSG antisense plasmid and cultured in vitro, their capacity to form blastocysts was severely impaired. Our results indicate that HSG plays an essential role in mouse preimplantation development.
Jiang
Guang-Jian
GJ
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
Pan
Lei
L
Huang
Xiu-Ying
XY
Han
Mei
M
Wen
Jin-Kun
JK
Sun
Fang-Zhen
FZ
D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D015153:Blotting, Western; D049109:Cell Proliferation; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D020558:GTP Phosphohydrolases / Q000235:genetics* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D051381:Rats; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism
D016376:Oligonucleotides, Antisense; D014404:Tubulin; D020558:GTP Phosphohydrolases; C488751:Mfn2 protein, mouse