Microplate nitrate reductase assay versus Alamar Blue assay for MIC determination of Mycobacterium tuberculosis.
A rapid colorimetric, microplate based nitrate reductase assay (NRA) method for minimum inhibitory concentration (MIC) determination of clinical isolates of Mycobacterium tuberculosis was compared with the microplate Alamar Blue assay (MABA), presently in vogue. Reproducible results were obtained in 7 days by NRA. The NRA method was found to be inexpensive, suitable for MIC determination against M. tuberculosis and can be suggested as an ideal substitute to MABA.
Kumar
M
M
Biotechnology Division, Regional Research Laboratory, Jammu Tawi, India.
Khan
I A
IA
Verma
V
V
Qazi
G N
GN
D001681:Biological Assay / Q000191:economics / Q000379:methods; D003362:Cost-Benefit Analysis; D024881:Drug Resistance, Bacterial; D007202:Indicators and Reagents; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D010078:Oxazines; D014397:Tuberculosis, Pulmonary / Q000188:drug therapy*; D014966:Xanthenes
D007202:Indicators and Reagents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin