title: Simultaneous choroidal tuberculoma and epididymo-orchitis caused by Mycobacterium tuberculosis.
abstract: OBJECTIVE
  To report positive polymerase chain reaction (PCR) for Mycobacterium tuberculosis from the vitreous and epididymal fluid in a patient of choroidal tuberculoma and epididymitis.
  
  
  METHODS
  Interventional case report.
  
  
  METHODS
  
  
  
  METHODS
  Institutional setting. intervention: Vitreous and epididymal fluid tap subjected to PCR for primer IS 6110 sequence specific for M. tuberculosis. Purification of DNA product and sequencing on an automated DNA sequencer. Institution of antitubercular therapy.
  
  
  METHODS
  Matching of sequence with NCBI's data bank response to antitubercular therapy.
  
  
  RESULTS
  DNA products from vitreous and epidiymal fluid matched with IS 6110 sequence of M. tuberculosis. Choroidal granuloma and epididymitis responded to antitubercular therapy.
  
  
  CONCLUSIONS
  Simultaneous choroidal tuberculoma and epididymitis resulted from an infection by M. tuberculosis, and both matched with IS 6110 sequence of M. tuberculosis.
authors.affiliation: Department of Ophthalmology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Vishali
authors.initials: V
authors.lastname: Gupta
authors.forename: Amod
authors.initials: A
authors.lastname: Gupta
authors.forename: Sunil
authors.initials: S
authors.lastname: Arora
authors.forename: Naresh
authors.initials: N
authors.lastname: Sachdeva
authors.forename: Pardeep
authors.initials: P
authors.lastname: Bambery
mesh_terms: D000328:Adult; D000995:Antitubercular Agents / Q000627:therapeutic use; D001826:Body Fluids / Q000382:microbiology; D015862:Choroid Diseases / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004823:Epididymitis / Q000382:microbiology*; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D014375:Tuberculoma / Q000382:microbiology*; D014392:Tuberculosis, Ocular / Q000382:microbiology*; D014822:Vitreous Body / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial