Measured depletion of ions at the biomembrane interface.
Expected from theory and simulations, depletion of ions at fuzzy biomembrane interfaces has long eluded experiments. Here, we show how salt exclusion can be accurately measured by surprisingly simple yet accurate benchtop measurements. Multilamellar aggregates of common phospholipids sink in low salt but float in salt solutions that are much less dense than the lipid itself. By manipulating bath and lipid densities, using heavy water and varied lipid chain length, we obtain accurate exclusion curves over a wide range of KCl and KBr concentrations. While maintaining a constant width at low salt, the exclusion layer decreases in high salt, following the Debye screening length. Consistent with interfacial accumulation of polarizable ions, bromide salts are less excluded than chloride, with an attraction of approximately 2kBT per Br- ion. So far neglected in theoretical descriptions, the competition between salt exclusion and binding is critical to understanding membrane interactions and specific ionic effects.
Petrache
Horia I
HI
Laboratory of Physical and Structural Biology, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA. petrachh@mail.nih.gov
Kimchi
Itamar
I
Harries
Daniel
D
Parsegian
V Adrian
VA
D001965:Bromides / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry*; D011189:Potassium Chloride / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D001965:Bromides; D007477:Ions; D008563:Membrane Lipids; D017680:Potassium Compounds; D014867:Water; D011189:Potassium Chloride; C039004:potassium bromide