Optimization of microwave-assisted extraction followed by solid phase micro extraction and gas chromatography-mass spectrometry detection for the assay of some semi volatile organic pollutants in sebum.
Methodology using MAE/SPME/GC-MS is being pursued for the analysis of organic pollutants in sebum. The microwave-assisted extraction (MAE) of standards of semi volatile organic pollutants from sebum was optimized. All compounds were extracted from sebum with recoveries analyzed by GC/MS ranging from 94% to 100% under the optimum MAE conditions: 10mL acetone-hexane (2:1), 60 degrees C, and 10 min microwave heating. To improve the detection limits a SPME procedure was optimized. Linearity ranged from 0.70 ppb to 25 ppb. R.S.D. were in the range of 1-23% for the SPME step. Preliminary real samples were analyzed and a range of compounds was detected. The optimized MAE/SPME/GC-MS methodology promises to be useful for different applications.
Díaz-Vázquez
Liz M
LM
Department of Chemistry, University of Puerto Rico, Río Piedras Campus, P.O. Box 23346, San Juan 00931-3346, Puerto Rico. lizvazquez8@hotmail.com
García
Oscar
O
Velázquez
Zorangelys
Z
Marrero
Ivelisse
I
Rosario
Osvaldo
O
D004785:Environmental Pollutants / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007202:Indicators and Reagents; D008872:Microwaves; D012629:Sebum / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014835:Volatilization
D004785:Environmental Pollutants; D007202:Indicators and Reagents