[Effects of compositions of Mahuang decoction on chemotaxis and leukotriene production from neutrophils in rats].
OBJECTIVE
To explore the regularity of recipe composition by observing inhibitory effects of disassembled compositions of Mahuang decoction (MHD) on chemotaxis and leukotriene production from neutrophils in rats.


METHODS
Neutrophil aggregation was induced by intraperitoneal injection of glycogen in rats. Intraperitoneal lavage fluid (PLF) was collected and neu-trophils were removed. Neutrophils were stimulated by calciumionophore A23187 in vitro to produce leukotriene B4. The concentrations of leukotriene B4 was measured by reversed-phase high performance liquid chromatography(HPLC), chemotatic chamber assay was used to investigate the regulative role of MHD on chenmotaxis of the neutrophils in response to LPS stimulation.


RESULTS
Disassembled compositions of MHD could inhibite chemotaxis and leukotriene production from neutrophils in rats. Inhibitory effects of MHD on mast cells were different.


CONCLUSIONS
MHD has significantly inhibitory effects on chemotaxis and leukotriene production from neutrophils in rats. The original formula (MHD) works best. These results have confirmed the rationality and scientific level of MHD.
Liu
Yong-Gang
YG
Key laboratory of Pharmaceutical of Materia Medica, First Military University, Guangzhou 510515, China.
Luo
Jia-Bo
JB
He
Feng
F
D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D007975:Leukotriene B4 / Q000096:biosynthesis*; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007975:Leukotriene B4