Distribution of two selenonucleosides among the selenium-containing tRNAs from Methanococcus vannielii.
Naturally occurring seleno-tRNAs from Methanococcus vannielii account for 13-20% of the total tRNA population. Two different selenium-modified nucleosides were detected in these seleno-tRNAs. Of the total selenium incorporated, about 60% is present in 5-methylaminomethyl-2-selenouridine, and the other 40% occurs in a second selenonucleoside with spectral characteristics typical of a derivative of 2-selenouridine. The 5-methylaminomethyl-2-selenouridine was found in the seleno-tRNA species present in the early fractions of a linear salt gradient elution profile from a reversed-phase chromatographic system 5 (RPC-5) column, whereas the second selenonucleoside occurred in the tRNA species eluted late in the profile.
Ching
W M
WM
Laboratory of Biochemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20205.
Wittwer
A J
AJ
Tsai
L
L
Stadtman
T C
TC
D002851:Chromatography, High Pressure Liquid; D017017:Methanococcus / Q000737:chemistry*; D009705:Nucleosides / Q000302:isolation & purification*; D016566:Organoselenium Compounds / Q000302:isolation & purification*; D012343:RNA, Transfer / Q000737:chemistry*; D014529:Uridine / Q000031:analogs & derivatives* / Q000302:isolation & purification*
C502847:2-selenouridine; D009705:Nucleosides; D016566:Organoselenium Compounds; C366009:seleno-tRNA; C043608:5-((methylamino)methyl)-2-selenouridine; D012343:RNA, Transfer; D014529:Uridine