title: Transcytosis of NgCAM in epithelial cells reflects differential signal recognition on the endocytic and secretory pathways.
abstract: NgCAM is a cell adhesion molecule that is largely axonal in neurons and apical in epithelia. In Madin-Darby canine kidney cells, NgCAM is targeted to the apical surface by transcytosis, being first inserted into the basolateral domain from which it is internalized and transported to the apical domain. Initial basolateral transport is mediated by a sequence motif (Y(33)RSL) decoded by the AP-1B clathrin adaptor complex. This motif is a substrate in vitro for tyrosine phosphorylation by p60src, a modification that disrupts NgCAM's ability to interact with clathrin adaptors. Based on the behavior of various NgCAM mutants, it appears that after arrival at the basolateral surface, the AP-1B interaction site is silenced by phosphorylation of Tyr(33). This slows endocytosis and inhibits basolateral recycling from endosomes, resulting in NgCAM transcytosis due to a cryptic apical targeting signal in its extracellular domain. Thus, transcytosis of NgCAM and perhaps other membrane proteins may reflect the spatial regulation of recognition by adaptors such as AP-1B.
authors.affiliation: Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, CT 06520, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Anderson
authors.forename: Sandra
authors.initials: S
authors.lastname: Maday
authors.forename: Jeff
authors.initials: J
authors.lastname: Sfakianos
authors.forename: Michael
authors.initials: M
authors.lastname: Hull
authors.forename: Bettina
authors.initials: B
authors.lastname: Winckler
authors.forename: David
authors.initials: D
authors.lastname: Sheff
authors.forename: Heike
authors.initials: H
authors.lastname: Fölsch
authors.forename: Ira
authors.initials: I
authors.lastname: Mellman
mesh_terms: D033961:Adaptor Protein Complex 1 / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D016764:Cell Polarity; D003593:Cytoplasm / Q000378:metabolism; D004285:Dogs; D004705:Endocytosis; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D008969:Molecular Sequence Data; D019000:Phosphotyrosine / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D015398:Signal Transduction
chemical_list: D033961:Adaptor Protein Complex 1; D015815:Cell Adhesion Molecules; D019000:Phosphotyrosine