title: Lewis blood genotypes of peptic ulcer and gastric cancer patients in Taiwan.
abstract: OBJECTIVE
  The Lewis b (Le(b)) antigen has been implicated as a possible binding site for attachment of Helicobacter pylori (H pylori) to gastric mucosa. However, studies both supporting and denying this association have been reported in the literature. Differences in secretor (Se) genotype have been suggested as a possible reason for previous discrepancies. Therefore, we investigated the relationship between Le and Se genotypes and H pylori infection rates in people with peptic ulcer or gastric cancer.
  
  
  METHODS
  Peripheral blood samples were obtained from 347 patients with endoscopic evidence of peptic ulcer disease (235 cases of duodenal ulcer, 62 of gastric ulcer, and 50 of combined duodenal ulcer/ gastric ulcer) and 51 patients with gastric cancer on endoscopy. Peripheral blood specimens from 101 unrelated normal volunteers were used as controls. Lewis phenotype was determined using an antibody method, whereas Le and Se genotypes were determined by DNA amplification and restriction enzyme analysis. Gastric or duodenal biopsies taken from patients with endoscopic evidence of peptic ulcer or gastric cancer were cultured for H pylori. Isolates were identified as H pylori by morphology and production of urease and catalase. The H pylori infection status was also evaluated by rapid urease test (CLO test), and urea breath test ((13)C-UBT). Results of studies were analyzed by chi-square test (taken as significant).
  
  
  RESULTS
  H pylori was isolated from 83.7% (303/347) of patients with peptic ulcer disease. Statistical analysis did not show any significant difference in Lewis phenotype or genotype between patients with and without H pylori infection. No significant association was found between Lewis genotype and peptic ulcer or gastric cancer.
  
  
  CONCLUSIONS
  Lewis blood genotype or phenotype may not play a role in the pathogenesis of H pylori infection. However, bacterial strain differences and the presence of more than one attachment mechanism may limit the value of epidemiological studies in elucidating this matter.
authors.affiliation: Blood Bank, and Division of Hematology/Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Shih-Chuan 1st Road, Kaohsiung, Taiwan, China.
authors.forename: Chi-Jung
authors.initials: CJ
authors.lastname: Yei
authors.forename: Jan-Gowth
authors.initials: JG
authors.lastname: Chang
authors.forename: Mu-Chin
authors.initials: MC
authors.lastname: Shih
authors.forename: Sheng-Fung
authors.initials: SF
authors.lastname: Lin
authors.forename: Chao-Sung
authors.initials: CS
authors.lastname: Chang
authors.forename: Fu-Tsong
authors.initials: FT
authors.lastname: Ko
authors.forename: Kuang-Yang
authors.initials: KY
authors.lastname: Lin
authors.forename: Ta-Chih
authors.initials: TC
authors.lastname: Liu
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D016481:Helicobacter Infections / Q000235:genetics; D016480:Helicobacter pylori; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000235:genetics*; D010437:Peptic Ulcer / Q000235:genetics*; D015995:Prevalence; D015183:Restriction Mapping; D013276:Stomach Ulcer / Q000453:epidemiology / Q000235:genetics*; D013624:Taiwan / Q000453:epidemiology
chemical_list: D000017:ABO Blood-Group System; D017931:DNA Primers; D007983:Lewis Blood Group Antigens