Utilisation of crown ethers in microemulsion electrokinetic chromatography for the separation of inorganic cations.
A new method to influence the separation selectivity of inorganic cations in capillary electrophoresis is presented. This method combines the use of certain crown ethers to form complexes with a specific cation (changing its ionic radius/charge ratio and thereby its electrophoretic mobility) with partitioning of the crown ether/analyte complex between an aqueous phase and a pseudo-stationary phase, such as the oil droplet of a microemulsion. Several microemulsions, including uncharged oil droplets and oil droplets with different degrees of surface charge were tested to evaluate their ability to improve the separation of the selected analytes.
Cook
Helmy A
HA
Institute of Analytical Chemistry, Johannes Kepler University Linz, Altenbergerstr. 69, A-4040 Linz, Austria.
Klampfl
Christian W
CW
Buchberger
Wolfgang
W
D002412:Cations / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D043844:Crown Ethers / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D004655:Emulsions / Q000737:chemistry; D015203:Reproducibility of Results
D002412:Cations; D043844:Crown Ethers; D004655:Emulsions