title: Identification of quantitative trait loci for susceptibility to mouse adenovirus type 1.
abstract: Adult SJL/J mice are highly susceptible to mouse adenovirus type 1 (MAV-1) infections, whereas other inbred strains, including BALB/cJ, are resistant (K. R. Spindler, L. Fang, M. L. Moore, C. C. Brown, G. N. Hirsch, and A. K. Kajon, J. Virol. 75:12039-12046, 2001). Using congenic mouse strains, we showed that the H-2(s) haplotype of SJL/J mice is not associated with susceptibility to MAV-1. Susceptibility of MAV-1-infected (BALB/cJ x SJL/J)F(1) mice was intermediate between that of SJL/J mice and that of BALB/cJ mice, indicating that susceptibility is a genetically controlled quantitative trait. We mapped genetic loci involved in mouse susceptibility to MAV-1 by analysis of 192 backcross progeny in a genome scan with 65 simple sequence length polymorphic markers. A major quantitative trait locus (QTL) was detected on chromosome 15 (Chr 15) with a highly significant logarithm of odds score of 21. The locus on Chr 15 alone accounts for 40% of the total trait variance between susceptible and resistant strains. QTL modeling of the data indicated that there are a number of other QTLs with small effects that together with the major QTL on Chr 15 account for 54% of the trait variance. Identification of the major QTL is the first step in characterizing host genes involved in susceptibility to MAV-1.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, 48109-0620, USA.
authors.forename: Amanda R
authors.initials: AR
authors.lastname: Welton
authors.forename: Elissa J
authors.initials: EJ
authors.lastname: Chesler
authors.forename: Carla
authors.initials: C
authors.lastname: Sturkie
authors.forename: Anne U
authors.initials: AU
authors.lastname: Jackson
authors.forename: Gwen N
authors.initials: GN
authors.lastname: Hirsch
authors.forename: Katherine R
authors.initials: KR
authors.lastname: Spindler
mesh_terms: D000256:Adenoviridae; D000257:Adenoviridae Infections / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006183:H-2 Antigens / Q000235:genetics; D051379:Mice; D020297:Mice, Congenic / Q000821:virology; D040641:Quantitative Trait Loci
chemical_list: D006183:H-2 Antigens