Design and expression of oligomeric fibronectin fusion protein: a strategy for enhancing cell adhesion activity.
Cell adhesion to extracellular matrices, including fibronectin, results in clustering of integrins in focal adhesions. To promote the clustering of fibronectin and thus enhance its activity at the sites of focal adhesion formation, we have engineered a fusion protein containing recombinant fibronectin fragment (hFN) connected to the tetramerization helix domain of lac repressor for oligomeric assembly. Purified Lac-hFN fusion protein exhibited significant increase of cell adhesion and proliferation of GF cells compared with hFN alone (p < 0.05).
Chung
Chong-Pyoung
CP
Intellectual Biointerface Engineering Center and Department of Periodontology, Seoul National University College of Dentistry, Korea.
Jang
Jun-Hyeog
JH
D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods; D019281:Dimerization; D004926:Escherichia coli / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005353:Fibronectins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005881:Gingiva / Q000166:cytology; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics; D013997:Time Factors
D005353:Fibronectins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins