title: Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates.
abstract: A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of K. pneumoniae isolates.
authors.affiliation: Unité Biodiversité des Bactéries Pathogènes Emergentes (U389 INSERM), Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris, Cedex 15, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Diancourt
authors.forename: Virginie
authors.initials: V
authors.lastname: Passet
authors.forename: Jan
authors.initials: J
authors.lastname: Verhoef
authors.forename: Patrick A D
authors.initials: PA
authors.lastname: Grimont
authors.forename: Sylvain
authors.initials: S
authors.lastname: Brisse
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D003428:Cross Infection / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D021521:Ribotyping; D017422:Sequence Analysis, DNA