[Antitumor effect of diallyl disulfide on human gastric cancer MGC803 cells xenograft in nude mice].
OBJECTIVE
Diallyl disulfide (DADS) can inhibit growth of various cancer cell lines in vitro, but little is known about its in vivo antitumor effect. This study was designed to investigate effect of DADS on growth of human gastric carcinoma MGC803 cells xenograft in BALB/C nude mice.


METHODS
MGC803 cells, with or without 1-day treatment of DADS (30 mg/L), were subcutaneously transplanted into the right axial regions of nude mice. The xenograft tumor growth in mice was observed after in vitro treatment or intraperitoneal injection of DADS. Proliferating cell nuclear antigen (PCNA) expression was detected by Western blot.


RESULTS
No xenograft tumor was observed in the nude mice inoculated with DADS-treated MGC803 cells. When injected with 50, 100, and 200 mg/kg of DADS, the inhibition rate of tumor growth in the nude mice inoculated with untreated MGC803 cells were 27.8%, 66.1%, and 73.0%; the expression of PCNA was inhibited.


CONCLUSIONS
DADS could suppress incidence of human gastric carcinoma xenograft in BALB/C nude mice, and inhibit growth of transplanted tumor.
Xiang
Shu-Lin
SL
Cancer Insititue, Nanhua University, Hengyang, Hunan, P. R. China.
Xiao
Xiao-Lan
XL
Ling
Hui
H
Liao
Qian-Jin
QJ
Zhou
Xiu-Tian
XT
Dong
Lin
L
Su
Qi
Q
D000498:Allyl Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004220:Disulfides / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*
D000498:Allyl Compounds; D000970:Antineoplastic Agents; D004220:Disulfides; D018809:Proliferating Cell Nuclear Antigen; C028009:diallyl disulfide