title: Genetic characterization of Cryptosporidium isolates from ringed seals (Phoca hispida) in Northern Quebec, Canada.
abstract: This study reports the molecular characterization of Cryptosporidium spp. isolates identified from intestinal contents of ringed seals (Phoca hispida) from Nunavik (Quebec, Canada). Cryptosporidium spp. fragments of 18S rRNA, HSP-70, and actin loci were amplified by PCR from seal intestinal contents. PCR-positive specimens were sequenced and compared with other Cryptosporidium species and genotypes reported previously. Sequence analysis showed the presence of C. muris and 2 novel genotypes in ringed seals.
authors.affiliation: Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute Agricultural Research Service, United States Department of Agriculture, Building 173, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA.
authors.forename: Mónica
authors.initials: M
authors.lastname: Santín
authors.forename: Brent R
authors.initials: BR
authors.lastname: Dixon
authors.forename: Ronald
authors.initials: R
authors.lastname: Fayert
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D016054:DNA, Protozoan / Q000737:chemistry; D005260:Female; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007422:Intestines / Q000469:parasitology; D008297:Male; D008969:Molecular Sequence Data; D046049:Phoca / Q000469:parasitology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011792:Quebec; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D016415:Sequence Alignment / Q000662:veterinary; D015047:Zoonoses / Q000469:parasitology / Q000635:transmission
chemical_list: D000199:Actins; D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D012337:RNA, Ribosomal, 18S