Protein phosphorylation is a prerequisite for intracellular Ca2+ release and ion channel control by nitric oxide and abscisic acid in guard cells.
Recent work has indicated that nitric oxide (NO) and its synthesis are important elements of signal cascades in plant-pathogen defence, and are a prerequisite for drought and abscisic acid (ABA) responses in Arabidopsis thaliana and Vicia faba guard cells. NO regulates inward-rectifying K+ channels and Cl- channels of Vicia guard cells via intracellular Ca2+ release. However, its integration with related signals, including the actions of serine-threonine protein kinases, is less well defined. We report here that the elevation of cytosolic-free [Ca2+] ([Ca2+]i) mediated by NO in guard cells is reversibly inhibited by the broad-range protein kinase antagonists staurosporine and K252A, but not by the tyrosine kinase antagonist genistein. The effects of kinase antagonism translate directly to a loss of NO-sensitivity of the inward-rectifying K+ channels and background (Cl- channel) current, and to a parallel loss in sensitivity of the K+ channels to ABA. These results demonstrate that NO-dependent signals can be modulated through protein phosphorylation upstream of intracellular Ca2+ release, and they implicate a target for protein kinase control in ABA signalling that feeds into NO-dependent Ca2+ release.
Sokolovski
Sergei
S
Laboratory of Plant Physiology and Biophysics, Bower Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
Hills
Adrian
A
Gay
Rob
R
Garcia-Mata
Carlos
C
Lamattina
Lorenzo
L
Blatt
Michael R
MR
D000040:Abscisic Acid / Q000502:physiology*; D002118:Calcium / Q000502:physiology*; D020013:Calcium Signaling / Q000502:physiology; D002227:Carbazoles / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology; D019833:Genistein / Q000494:pharmacology; D026121:Indole Alkaloids; D007473:Ion Channels / Q000502:physiology*; D008564:Membrane Potentials; D009569:Nitric Oxide / Q000502:physiology*; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D019311:Staurosporine / Q000494:pharmacology; D031307:Vicia faba / Q000166:cytology / Q000502:physiology*
D002227:Carbazoles; D026121:Indole Alkaloids; D007473:Ion Channels; D010940:Plant Proteins; D047428:Protein Kinase Inhibitors; D009569:Nitric Oxide; D000040:Abscisic Acid; C049985:staurosporine aglycone; D019833:Genistein; D011494:Protein Kinases; D019311:Staurosporine; D002118:Calcium