Solid-phase extraction and liquid chromatography-electrospray mass spectrometric analysis of saponins in a Chinese patent medicine of formulated Salvia miltiorrhizae and Panax notoginseng.
In this paper, a method using solid-phase extraction (SPE) and HPLC/ESI-MS(n) for the identification of the major saponins in "Danshen Dripping Pill", a Chinese patent medicine consisting of Salvia miltiorrhizae and Panax notoginseng, is described. Through solid-phase extraction process, the saponins in "Danshen Dripping Pill" were separated from the phenolic constituents of S. miltiorrhizae and, meanwhile, efficiently concentrated. Subsequently, these saponins were characterized by HPLC/ESI-MS(n) analysis. Based on the studies of MS and MS2 spectra and the comparison with reference compounds and literature data, a total of 19 saponins were identified.
Zhang
Haijiang
H
College of Pharmaceutical Sciences, Zhejiang University, 310027 Hangzhou, China.
Cheng
Yiyu
Y
D002853:Chromatography, Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D036145:Ginsenosides / Q000032:analysis; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts; D027561:Salvia miltiorrhiza; D012503:Saponins / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D010936:Plant Extracts; D012503:Saponins