title: Semisynthetic tellurosubtilisin with glutathione peroxidase activity.
abstract: Reaction of the hydroxyl group of serine-221 of subtilisin with phenylmethanesulfonylfluoride followed by nucleophilic substitution with sodium hydrogen telluride, a semisynthetic telluroprotein, tellurosubtilisin, was prepared. Tellurosubtilisin, which displays high substrate specificity for aromatic thiols, exhibits remarkable peroxidase activity and catalyzes the reduction of hydrogen peroxide by 3-carboxy-4-nitrobenzenethiol 20 000 times more efficiently than diphenyl diselenide.
authors.affiliation: Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Mao
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Dong
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
authors.forename: Xiangqiu
authors.initials: X
authors.lastname: Li
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Liu
authors.forename: Guimin
authors.initials: G
authors.lastname: Luo
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
mesh_terms: D002384:Catalysis; D005979:Glutathione Peroxidase / Q000737:chemistry*; D020860:Subtilisin / Q000138:chemical synthesis* / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry
chemical_list: D005979:Glutathione Peroxidase; D020860:Subtilisin; D013691:Tellurium