title: Effects of aromatase inhibitors on proliferation and apoptosis in eutopic endometrial cell cultures from patients with endometriosis.
abstract: OBJECTIVE
  To study the effect of letrozole (Let) and anastrozole (Anas) on apoptosis and cell proliferation in epithelial endometrial cells (EEC) from patients with endometriosis (EDT).
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Research institute and clinical fertility center.
  
  
  METHODS
  Eighteen women with untreated EDT.
  
  
  METHODS
  Biopsy specimens of eutopic endometrium were obtained from all subjects. Apoptosis and cell proliferation were examined in EEC after incubation with Let or Anas.
  
  
  METHODS
  Percentage of apoptotic cells (ApC) was evaluated by the acridine orange-ethidium bromide technique; cell proliferation was assessed by 3H-thymidine incorporation.
  
  
  RESULTS
  Treatment with Let 10 nM and Let 100 nM enhanced values of ApC in cultures from EDT patients. Epithelial endometrial cells treated with Anas 100 nM or Anas 500 nM showed a statistically significant induction on apoptosis levels. Cultures treated with Let 1 nM or Anas 50 nM did not show any significant differences in ApC levels compared with basal conditions. 3H-Thymidine uptake was down regulated by Let 10 nM and Let 100 nM. Similarly, Anas 100 nM and Anas 500 nM showed a significantly lower degree of cell proliferation in EEC. Lower concentrations of Let and Anas did not induce any significant change in cell proliferation rates.
  
  
  CONCLUSIONS
  Our results show that Let and Anas produced a significant and positive effect on apoptosis and cell proliferation on EEC from EDT patients. These findings support the further investigation of aromatase inhibitors as a treatment option in EDT.
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME), Hospìtal de Clínicas José de San Martín, Buenos Aires, Argentina. meresman@dna.uba.ar
authors.forename: Gabriela F
authors.initials: GF
authors.lastname: Meresman
authors.forename: Mariela
authors.initials: M
authors.lastname: Bilotas
authors.forename: Verónica
authors.initials: V
authors.lastname: Abello
authors.forename: Ricardo
authors.initials: R
authors.lastname: Buquet
authors.forename: Marta
authors.initials: M
authors.lastname: Tesone
authors.forename: Carlos
authors.initials: C
authors.lastname: Sueldo
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D047072:Aromatase Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004715:Endometriosis / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D004717:Endometrium / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D005260:Female; D006801:Humans; D011446:Prospective Studies; D018709:Statistics, Nonparametric
chemical_list: D047072:Aromatase Inhibitors