A comparative study of classical and biochemical endpoints for phytotoxicity testing of chlorobenzoic acids.
The phytotoxicity of chlorobenzoic acids (CBAs) was studied and the biochemical endpoints' suitability and sensibility was evaluated. Two terrestrial plant species in the same family were exposed to different concentrations of CBAS and tested their germination according to the guideline of Organization for Economic Cooperation and Development (OECP, 1984). The results showed that CBA dose-inhibition rate of classical endpoint had the distinct linear relationship in the range of 10%-50% inhibition rate for root elongation (p < 0.01), and the dose variances of CBAs had the greater influence on the inhibition rate of germination than on inhibition rate of root elongation. The CBA dose half effect concentration-inhibition rate of two antioxidant enzyme activity superoxide dismutase (SOD) and catalase (CAT) had the quadratic relationship, and CBA dose-inhibition rate of the peroxides (POD) activity had the linear relationship (p < 0.05). Comparing the half effect concentration (EC50) of two kinds of endpoints, the POD activity was more sensitive than classical endpoint, however, SOD and CAT activity were not sensitive in the experiment.
Li
Pei-Jun
PJ
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Beijing 110016, China.
Yin
Pei-Jie
PJ
Zhou
Qi-Xing
QX
Shi
Xing-Qun
XQ
Xiong
Xian-Zhe
XZ
D001565:Benzoates / Q000633:toxicity*; D001937:Brassica / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D023381:Endpoint Determination / Q000379:methods; D018525:Germination / Q000187:drug effects; D010545:Peroxides / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development; D012044:Regression Analysis; D013482:Superoxide Dismutase / Q000378:metabolism; D018675:Toxicity Tests / Q000379:methods
D001565:Benzoates; D010545:Peroxides; D002374:Catalase; D013482:Superoxide Dismutase