Harmonization of immunotoxicity guidelines in the ICH process--pathology considerations from the guideline Committee of the European Society of Toxicological Pathology (ESTP) .
As part of the ICH process of harmonization of testing guidelines for immunotoxicity, the European Society of Toxicologic Pathology (ESTP) has contributed to the scientific discussion on methods and evaluation of immunotoxicity studies with technical and scientific recommendations on toxicologic pathology. The weighing and sampling of immune organs is discussed taking into consideration specifically the value of lymph node weighing and the selection of appropriate lymph nodes for the detection of local and systemic effects. The different techniques of bone marrow preparation are considered for routine and extended investigations. Criteria are given for the gross and histopathological detection of effects in Peyer's patches. For the histopathological evaluation it is strongly recommended that each compartment within the different lymphoid organs is investigated separately and semiquantitatively since this approach has shown to increase the sensitivity and specificity of immunohistopathology.
Ruehl-Fehlert
Christine
C
Department of Toxicologic Pathology, Bayer HealthCare AG, Wuppertal, Germany. guidelines@eurotoxpath.org
Bradley
Alys
A
George
Catherine
C
Germann
Paul-Georg
PG
Bolliger
Anne Provencher
AP
Schultee
Agnes
A
D000818:Animals; D007107:Immune System / Q000187:drug effects* / Q000473:pathology; D007166:Immunosuppressive Agents / Q000633:toxicity*; D007391:International Cooperation; D010341:Pathology, Veterinary / Q000379:methods* / Q000592:standards; D012958:Societies, Scientific; D018675:Toxicity Tests / Q000379:methods* / Q000592:standards; D015262:Xenobiotics / Q000633:toxicity*
D007166:Immunosuppressive Agents; D015262:Xenobiotics