Isolation, solubilization, refolding, and chromatographic purification of human growth hormone from inclusion bodies of Escherichia coli cells: a case study.
Singh
Surinder M
SM
Product Development Cell, National Institute of Immunology, New Delhi, India.
Eshwari
A N S
AN
Garg
Lalit C
LC
Panda
Amulya K
AK
D002853:Chromatography, Liquid; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D019382:Human Growth Hormone / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D002479:Inclusion Bodies / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012995:Solubility
D011994:Recombinant Proteins; D019382:Human Growth Hormone