title: Presence and characterization of a mosaic genomic island which distinguishes sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- from E. coli O157:H7.
abstract: A mosaic genomic island comprising Shigella resistance locus (SRL) sequences flanked by segments of Escherichia coli O157:H7 strain EDL933 O islands 43, 81, and 82 was identified in sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H(-) strain 493/89. This mosaic island is absent from strain EDL933. PCR targeting the SRL-related sequence is a useful tool to distinguish SF EHEC O157:H(-) from EHEC O157:H7.
authors.affiliation: Institut für Hygiene, Universitätsklinikum Münster, Robert-Koch Strasse 41, D-48149 Münster, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Janka
authors.forename: Georg
authors.initials: G
authors.lastname: Becker
authors.forename: Anne-Katharina
authors.initials: AK
authors.lastname: Sonntag
authors.forename: Martina
authors.initials: M
authors.lastname: Bielaszewska
authors.forename: Ulrich
authors.initials: U
authors.lastname: Dobrindt
authors.forename: Helge
authors.initials: H
authors.lastname: Karch
mesh_terms: D015373:Bacterial Typing Techniques; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D005285:Fermentation; D044404:Genomic Islands; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D012703:Serotyping; D012760:Shigella / Q000187:drug effects / Q000235:genetics; D013012:Sorbitol / Q000378:metabolism*
chemical_list: D013012:Sorbitol