[Effect of oxygen and nitrogen active compounds on pH value in myometrium myocytes].
In suspension from a rat uterus using fluorescent pH1 probe BCECF-AM was established that the "apparent" pH1 value for this object was 6.72 +/- 0.02 at pHo = 7,4 (t = 25 degrees C, n = 5). Cell activation by 0.1 mM carbachol was accompanied by a rise in cytosolic pH up to 6.81 +/- 0.01. This process with different efficiency was inhibited by blockers of K(+)- and H(+)-channels: 0.1 mM 4-amidopyridine, tetraethilamonium and cadmium. The blockers of passive H(+)-transport do not inhibit completely alkalization of myoplasm that can testify in the favor of the non-channel way of passive transport H+ through plasmalemma. It was established that NO (0.1 mM sodium nitroprusside) and NO2- (10 nM) significantly enhanced the carbochol-induced rise of myoplasm pH in the cells from myometrium. It also was shown that carbachol-induced pH. in myocytes was increased by the addition of 10 nM H2O2. It was suggested that the studied compounds could influence Ca2+ transport systems by changing pH in myocytes.
Danylovych
Iu V
IuV
Tuhaĭ
V A
VA
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006859:Hydrogen / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism*; D009215:Myometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*; D051381:Rats
D009599:Nitroprusside; D009569:Nitric Oxide; D006859:Hydrogen; D002217:Carbachol; D006861:Hydrogen Peroxide; D010100:Oxygen; D002118:Calcium