title: Determination of leflunomide in tablets by high performance liquid chromatography.
abstract: In the present study, a reverse phase high performance liquid chromatography (HPLC) method was validated and applied for the determination of leflunomide in tablets. Chromatographic separation of leflunomide and oxazepam as an internal standard was carried out on a C(18) column (50 mm, 3 mm i.d.) using a mobile phase, consisting of methanol and water (60:40, v/v), at a flow rate of 0.5 ml min(-1) and UV detection at 260 nm. The retention times for oxazepam and leflunomide were 2.6 and 5.2 min, respectively. The validated quantification range of the method was 2.7 x 10(-6) to 5.5 x 10(-5) M for leflunomide. The results of the developed procedure in tablets were compared with those of UV spectrophotometry to assess active leflunomide content.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Yeniceli
authors.forename: Dilek
authors.initials: D
authors.lastname: Dogrukol-Ak
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Tuncel
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007555:Isoxazoles / Q000032:analysis*; D000077339:Leflunomide; D008956:Models, Chemical; D010076:Oxazepam / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007555:Isoxazoles; D013607:Tablets; D010076:Oxazepam; D000077339:Leflunomide