title: Investigation of Fe(III) chloride as a colour reagent for the spectrophotometric determination of bumetanide in the pure form and in preparations.
abstract: The formation and stability of the complex between bumetanide (3-butylamino-4-phenoxy-5-sulphamoylbenzoic acid) and Fe(III) ion was studied. It has been found that bumetanide reacts with Fe(III) chloride in the presence of ammonium thiocyanate at the pH 1.83-1.92 to form a violet complex soluble in chlorophorm, with the maximum absorbance at 514.5 nm. At the optimum experimental conditions the conditional stability constant of the complex was found to be log K' = 7.34. The Beer's law was obeyed up to the 160 microg x cm(-3) and the relative standard deviation varied from 0.406% to 0.475% (n = 10). The proposed method was found to be suitable for the accurate and sensitive analysis of bumetanide as a pure compound and from tables and ampoules.
authors.affiliation: Institute of Pharmaceutical Chemistry, Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Yugoslavia.
authors.forename: L
authors.initials: L
authors.lastname: Zivanovic
authors.forename: S
authors.initials: S
authors.lastname: Agatonovic-Kustrin
authors.forename: M
authors.initials: M
authors.lastname: Vasiljevic
authors.forename: D
authors.initials: D
authors.lastname: Radulovic
mesh_terms: D002034:Bumetanide / Q000032:analysis*; D002712:Chlorides; D004232:Diuretics / Q000032:analysis; D005290:Ferric Compounds; D007202:Indicators and Reagents; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods; D013607:Tablets
chemical_list: D002712:Chlorides; D004232:Diuretics; D005290:Ferric Compounds; D007202:Indicators and Reagents; D013607:Tablets; D002034:Bumetanide