Isolation of an N-acetyl-D-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity.
A lectin with antiproliferative activity towards human cancer cell lines and mitogenic towards human peripheral blood mononuclear cells was purified from the rhizomes of Arundo donax (Linn.) by affinity chromatography on N-acetyl-d-glucosamine linked to epoxy-activated sepharose-6B. The pure preparation apparently yielded a single band of approximately 15 kDa on SDS-PAGE, pH 8.3, under both reducing and non-reducing conditions. The molecular mass of native lectin was 32 kDa as determined by gel filtration chromatography. This showed the lectin to be a dimer, with subunits not held together by disulphide linkages. The A. donax lectin (ADL) agglutinated rabbit erythrocytes and the agglutination was inhibited by N-acetyl-d-glucosamine and its di- and trimer. The lectin was thermostable upto 55 degrees C and showed optimum activity in the range of pH 7.0-9.0 and comprised of 2.1% carbohydrate content.
Kaur
Amandeep
A
Department of Molecular Biology & Biochemistry, Guru Nanak Dev University, Amritsar 143 005, Punjab, India.
Singh
Jatinder
J
Kamboj
Sukhdev Singh
SS
Sexana
A K
AK
Pandita
Renu Moti
RM
Shamnugavel
M
M
D000117:Acetylglucosamine / Q000737:chemistry* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008934:Mitogens / Q000737:chemistry / Q000494:pharmacology; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D008517:Phytotherapy; D006109:Poaceae / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D037102:Lectins; D008934:Mitogens; D000117:Acetylglucosamine