Single-nucleotide polymorphism detection with "wire-like" DNA probes that display quasi "on-off" digital action.
Establishing a reliable genotyping protocol is a critical matter in post-sequence genetics. In this article, we describe a highly sensitive electrochemical detection of complementary DNAs (up to 43-mer) based on hole transport with molecular-scale, "wire-like" DNA probes. The presence of a single-base mismatch in the DNA duplexes caused a dramatic decrease in the electrochemical response. We applied this method to detect all of the possible transition and transversion SNPs and achieved "on-off"-type discrimination of fully complementary DNAs from their SNPs. Furthermore, naturally occurring polymorphisms, "hot spots" from the p53 gene, could clearly be distinguished from wild type by using our methodology.
Inouye
Masahiko
M
Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Japan. inouye@ms.toyama-mpu.ac.jp
Ikeda
Reona
R
Takase
Masayoshi
M
Tsuri
Takashi
T
Chiba
Junya
J
D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics*; D004563:Electrochemistry; D004566:Electrodes; D015394:Molecular Structure; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D015342:DNA Probes