Foldamers with hybrid biological and synthetic sequences as selective DNA fluorescent probes.
Foldable polymers with alternating single-strand deoxyribonucleic acid (ssDNA) and planar fluorescent organic chromophores can self-organize into folded nanostructures and hence are hybrid foldamers with biological sequences and synthetic properties. The biological sequence provides highly specific molecular recognition properties, while the physical properties of synthetic chromophores offer sensitive fluorescence detection. In this paper, we describe that rational designed hybrid foldamers exhibit potential in the detection of polynucleotides. Under strictly controlled laboratory conditions, fluorescence measurements indicate that configuration change due to binding of polynucleotides with one or two mismatched bases can be readily distinguished. These results shed light on the design and construction of nanostructured foldamers with actuator and sensory properties, which may find important applications as biological probes.
Wang
Wei
W
Department of Chemistry, Washington State University, Pullman, Washington 99164, USA.
Wan
Wei
W
Stachiw
Andrew
A
Li
Alexander D Q
AD
D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D007700:Kinetics; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D020641:Polymorphism, Single Nucleotide
D015342:DNA Probes; D005456:Fluorescent Dyes; D004247:DNA