The effect of three rad genes on survival, inter- and intragenic mitotic recombination in Saccharomyces. I. UV irradiation without photoreactivation or liquid-holding post-treatment.
The effect of UV irradiation on the survival, inter- and intragenic mitotic recombination of 3 diploid UV sensitive Saccharomyces mutants was studied and compared with the wild type RAD. These strains, homozygous for either the RAD, r1s rad 9-4, or rad 2-20 gene, have DRF values for survival of 1:1.6:3:20.6 respectively, at LD1. Their recombination behaviour is not correlated to their survival characteristics. The RAD, r1s, and rad 2-20 strains showed UV induced mitotic inter- and intragenic recombinants; the induction in the r1s diploid is ca. 100 times greater for both the inter- and intragenic recombinants than in the RAD strain. The rad 9-4 diploid produced no UV induced mitotic recombinants whatsoever, and is therefore considered to be a rec- mutation.
Kowalski
S
S
Zentralinstitut für Biochemie und Biophysik der Freien Universität Berlin.
Laskowski
W
W
D005800:Genes, Fungal; D009154:Mutation; D011995:Recombination, Genetic / Q000235:genetics* / Q000528:radiation effects; D012440:Saccharomyces / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays