Simultaneous measurement of superoxide generation and intracellular calcium ion of neutrophil-like culture cells.
We have developed a novel method for simultaneously measuring fluorescence and chemiluminescence. The generation of superoxide anion and the intracellular Ca(2+) ion concentration of neutrophil-like cells stimulated by agonists were measured in real time by our method. Our results were in agreement with the intracellular signalling in the neutrophils. We also found that the presence of Zn(2+) ion inhibited both the generation of superoxide anion and the influx of Ca(2+) ions.
Satozono
Hiroshi
H
Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamamatsu-City 434-8601, Japan. satozono@crl.hpk.co.jp
Kazumura
Kimiko
K
Okazaki
Sigetoshi
S
Hiramatsu
Mitsuo
M
D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D012680:Sensitivity and Specificity; D015398:Signal Transduction / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000032:analysis / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000494:pharmacology
D013481:Superoxides; C016658:phorbolol myristate acetate; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015032:Zinc; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium