A competition assay of magnesium affinity for EF-hand proteins based on the fluorescent indicator magnesium green.
Erickson
Jeffrey R
JR
Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Moerland
Timothy S
TS
D000814:Aniline Compounds; D000818:Animals; D001667:Binding, Competitive; D002151:Calorimetry / Q000379:methods; D002347:Carps; D020832:EF Hand Motifs; D005456:Fluorescent Dyes; D005998:Glycine / Q000031:analogs & derivatives; D007700:Kinetics; D008274:Magnesium / Q000737:chemistry* / Q000378:metabolism; D010320:Parvalbumins / Q000737:chemistry; D015203:Reproducibility of Results; D017186:Titrimetry; D014966:Xanthenes
D000814:Aniline Compounds; D005456:Fluorescent Dyes; D010320:Parvalbumins; D014966:Xanthenes; C453498:magnesium green; C059715:Fluo-3; D008274:Magnesium; D005998:Glycine