title: Cytoplasmic retention of the sex-determining factor SOX9 via the microtubule network.
abstract: SOX9 is a sex-determining factor which induces Sertoli cell differentiation and subsequent testis cord formation. It is expressed both in male and female undifferentiated gonads in the cytoplasmic compartment of pre-Sertoli cells. At the time of sexual differentiation, SOX9 moves into the nucleus of male pre-Sertoli cells whereas in female, it remains in the cytoplasm and then its expression decreases. To study the cytoplasmic localization of SOX9, we have analyzed its interaction with the cytoskeleton components. By treatment of NT2/D1 and transfected NIH3T3 cell lines and embryonic gonads with nocodazole, a drug depolymerizing the microtubules, we show that cytoplasmic retention of SOX9 requires the integrity of the microtubule network. Using biochemical experiments, we demonstrated that SOX9 is able to interact with microtubules in vitro and in vivo. On the other hand, we observed a complete male-specific reorganization of the microtubule network in epithelial Sertoli cells of the male embryonic gonad at the time of sexual differentiation and testis cord formation.
authors.affiliation: Department of Differentiation and Development, Institut de Génétique Humaine CNRS UPR1142, 141, rue de la Cardonille, 34396 Montpellier Cedex 05, France.
authors.forename: Safia
authors.initials: S
authors.lastname: Malki
authors.forename: Philippe
authors.initials: P
authors.lastname: Berta
authors.forename: Francis
authors.initials: F
authors.lastname: Poulat
authors.forename: Brigitte
authors.initials: B
authors.lastname: Boizet-Bonhoure
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D005260:Female; D006609:High Mobility Group Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D041681:NIH 3T3 Cells; D009924:Organ Culture Techniques; D010053:Ovary / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D055758:SOX9 Transcription Factor; D012708:Sertoli Cells / Q000378:metabolism; D019849:Sex Determination Processes; D013737:Testis / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
chemical_list: D006609:High Mobility Group Proteins; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C527933:Sox9 protein, mouse; D014157:Transcription Factors; D014404:Tubulin