[In situ hybridization analysis of mRNA expression of 5-Hydroxytryptamine4 receptor in intestinal mucosa mast cells].
OBJECTIVE
To verify 5-HT(4) receptor mRNA expression in intestinal mucosa mast cells (IMMC).


METHODS
IMMC was isolated from the whole intestines of normal rats by collagenase digestion and purified by percoll. In situ hybridization was performed to detect the expression of 5-HT(4) receptor mRNA in IMMC.


RESULTS
Evidently positive staining was shown in the cytoplasm of IMMC.


CONCLUSIONS
We have verified the expression of 5-HT(4) receptor mRNA in IMMC for the first time and provided evidence for further research.
Chi
Yan
Y
Department of Gastroenterology, Peking University First Hospital, Beijing 100034, China.
Liu
Xin-guang
XG
D000818:Animals; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D011985:Receptors, Serotonin