Protective effect of Toki-shakuyaku-san on amyloid beta25-35-induced neuronal damage in cultured rat cortical neurons.
Amyloid beta protein (Abeta) is the major component of senile plaques, the pathological hallmark of the neurodegeneration associated with Alzheimer's disease (AD). This study investigated the effect of Toki-shakuyaku-san (TSS), a traditional medicine, on Abeta25-35-induced neuronal death and lipid peroxidation assessed by measuring lactate dehydrogenase (LDH) and malondialdehyde (MDA), respectively. Abeta25-35 at 10 microM induced neuronal damage and increased the LDH and MDA. TSS at concentrations of 100 and 300 microg/mL significantly reduced the Abeta25-35-induced neuronal death and the lipid peroxidation. These results suggest that TSS has a protective effect against Abeta25-35-induced neuronal damage. TSS may be beneficial for the treatment of AD.
Egashira
Nobuaki
N
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1, Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
Iwasaki
Katsunori
K
Akiyoshi
Yuki
Y
Takagaki
Yuki
Y
Hatip-Al-Khatib
Izzettin
I
Mishima
Kenichi
K
Kurauchi
Kouji
K
Ikeda
Tomoaki
T
Fujiwara
Michihiro
M
D016229:Amyloid beta-Peptides; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D010446:Peptide Fragments; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar
D016229:Amyloid beta-Peptides; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); C413220:toki-shakuyaku-san; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase