Influence of dietary protein supply on resistance to experimental infections with Haemonchus contortus in Ile de France and Santa Ines lambs.
The effect of Haemonchus contortus infection in sheep fed with a moderate and high protein content diet was evaluated in two breeds of sheep. Forty-eight Ile de France and Santa Ines lambs were maintained indoors since birth, in worm-free conditions. The lambs were allocated after weaning in four groups of six animals per breed, which were either infected or remain uninfected and given access to either a moderately or highly metabolizable protein diet. The moderately and highly metabolizable protein diets were calculated to supply 75 and 129 g metabolizable protein per kg of dry matter (MP/kg DM), respectively. The infection consisted of a trickle infection with 300 infective larvae, three times a week, for 12 weeks. Significant differences were observed for mast cell, globule leukocyte and eosinophil counts in the abomasal mucosa of the infected groups compared to the control of both breeds (P<0.05), regardless of the diet supplied. Significantly higher IgA anti-L5 antibody was detected in the infected Santa Ines groups than in the infected Ile de France groups (P<0.05). Increased metabolizable protein supply resulted in larger body weight gain and higher packed cell volumes for both breeds (P<0.05). Both breeds showed an increased ability to withstand the pathophysiological effects of H. contortus infection when given access to the highly metabolizable protein diet. However, increased metabolizable protein supply resulted in reduced worm burdens in Santa Ines lambs but not in the Ile de France lambs (P<0.05). The present results show that the increase in protein content in growing lamb diets may benefit resistance and resilience to gastrointestinal parasites but that these benefits may vary among breeds.
Bricarello
P A
PA
Departamento de Parasitologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brazil. bricarellopa@yahoo.com.br
Amarante
A F T
AF
Rocha
R A
RA
Cabral Filho
S L
SL
Huntley
J F
JF
Houdijk
J G M
JG
Abdalla
A L
AL
Gennari
S M
SM
D000018:Abomasum / Q000469:parasitology; D000818:Animals; D001772:Blood Cell Count / Q000662:veterinary; D001835:Body Weight / Q000502:physiology; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000469:parasitology; D005260:Female; D005753:Gastric Mucosa / Q000469:parasitology; D005767:Gastrointestinal Diseases / Q000276:immunology / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D020022:Genetic Predisposition to Disease; D006188:Haemonchiasis / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000254:growth & development*; D006400:Hematocrit / Q000662:veterinary; D006632:Histamine / Q000032:analysis; D006651:Histocytochemistry / Q000662:veterinary; D016014:Linear Models; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000378:metabolism / Q000469:parasitology*
D004044:Dietary Proteins; D006632:Histamine