[Inhibition of proliferation and induction of apoptosis by tanshinone II A in NCI-H460 cell].
OBJECTIVE
To investigate the inhibition of tanshinone II A in NCI-H460 cell line and its mechanism.


METHODS
MTT was used to measure the levels of the proliferation of NCI-H460 cultured with tanshinone II A in different concentrations. The effects of tanshinone II A on cell cycle of NCI-H460 were observed by FCM. After treated with tanshinone II A for 48h, the level of mRNA of bcl-2 and C-myc in NCI-H460 was tested by RT-PCR method.


RESULTS
The proliferation of NCI-H460 was obviously inhibited by tanshinone II A in a dose dependent manner. The outcome of FCM showed that the apoptotic cell rate was 1.2%, 3.4%, 7.7% respectively, when cultured with tanshinone II A at 0.5 microg/ml for 24, 48, 72 h. The apoptotic cell rate was 2.6%, 5.9%, 13.4%, when cultured with tanshinone II A at 1.0 microg/ml for 24, 48, 72 h. The apoptotic cell rate was 4.1%, 8.7%, 37.6%, when cultured with tanshinone II A at 2.0 microg/ml for 24, 48, 72 h. The outcome of RT-PCR showed that the expression of proto-onco gene bcl-2 and C-myc was notably decrease, after cultured with tanshinone II A for 48 h.


CONCLUSIONS
Tanshinone IIA can inhibit the proliferation of NCI-H460 and induce the apoptosis of the cell.
Hu
Haiyan
H
Hematologic Institute, Medical College, Jinan University, Guangzhou 510623.
Zhang
Yuan
Y
Huang
Feng
F
Deng
Hui
H
D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D010616:Phenanthrenes / Q000008:administration & dosage / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D027561:Salvia miltiorrhiza / Q000737:chemistry*
D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D010616:Phenanthrenes; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C021751:tanshinone