title: Membrane properties of cationic liposomes composed of dipalmitoylphosphatidylcholine and dipalmityldimethylammonium bromide.
abstract: Cationic liposomes composed of dipalmitoylphosphatidylcholine (DPPC) and dipalmityldimethylammmonium bromide (DPAB) were prepared by the Bangham method and the effect of DPAB on the membrane properties was examined in terms of liposomal shape, particle size, trapping efficiency, surface potential and dispersibility. The dispersibility of the mixed DPPC/DPAB liposomes (the mole fraction of DPAB (XDPAB)>==0.05) was excellent and the dispersibility was maintained for 6 months, since the zeta-potential of the mixed liposomes was approximately +40 mV. The trapping efficiency of the mixed DPPC/DPAB liposomes (XDPAB=0.05) was 10 times greater than that of the DPPC liposomes, and the value was largest among the mixed liposomes (XDPAB=0-1.0). Freeze-fracture electron micrographs indicated that the shape of the mixed DPPC/DPAB liposomes (XDPAB=0.05) was that of large unilamellar vesicles (LUVs) with a diameter of approximately 2 microm, while the shape of the DPPC liposomes was that of multilamellar vesicles (MLVs). The mixed liposomes had, therefore, a high trapping efficiency. Furthermore, the shape of the mixed DPPC/DPAB liposomes (XDPAB=0.75) was also that of LUVs with a diameter of approximately 2 microm and these had a high trapping efficiency. Whereas, the particle size (500 nm) of the mixed DPPC/DPAB liposomes (XDPAB=0.25) was smaller than that of the former and had the minimum trapping efficiency. The phase transition temperature of the liposomal bilayer membranes indicated a maximum value at 0.25-0.30 mole fractions of DPAB. These facts were considered to be due to the fact that DPPC and DPAB, whose molar ratio was 7.5:2.5, were tightly packed in the liposomal bilayer membranes and that the curvature of the liposomal particle was resultantly large. Nevertheless, LUVs having a high trapping efficiency were easily obtained by mixing a small amount of DPAB with the DPPC.
authors.affiliation: School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-cho, Nobeoka-city, Miyazaki 882-8508, Japan. s.yokoyama@phoenix.ac.jp
authors.forename: Shoko
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Akiko
authors.initials: A
authors.lastname: Inagaki
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Tsubaki
authors.forename: Hideki
authors.initials: H
authors.lastname: Sakai
authors.forename: Masahiko
authors.initials: M
authors.lastname: Abe
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D001965:Bromides / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D005614:Freeze Fracturing; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008854:Microscopy, Electron; D008956:Models, Chemical; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry*; D013997:Time Factors
chemical_list: D001965:Bromides; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D015060:1,2-Dipalmitoylphosphatidylcholine