title: Analysis of the AhR, ARNT, and AhRR gene polymorphisms: genetic contribution to endometriosis susceptibility and severity.
abstract: OBJECTIVE
  To explore whether polymorphisms in AhR, ARNT, and AhRR contribute to endometriosis susceptibility and severity.
  
  
  METHODS
  Case control study.
  
  
  METHODS
  Hospital.
  
  
  METHODS
  One hundred thirty-eight Japanese women with or without endometriosis, diagnosed endoscopically.
  
  
  METHODS
  Endoscopic laparoscopy, with blood samples for genotyping obtained before the laparoscopic examination for genomic DNA extraction from peripheral leukocytes.
  
  
  METHODS
  AhR, ARNT, and AhRR polymorphisms were genotyped using real-time polymerase chain reaction (PCR) analysis. Odds ratios and 95% confidence intervals were calculated for AhR, ARNT, and AhRR genotypes to evaluate the risk of endometriosis. Associations between these polymorphisms and stage of endometriosis were also examined.
  
  
  RESULTS
  The C/G + G/G genotypes at codon 185 of AhRR showed a statistically significant association with risk of endometriosis (adjusted odds ratio, 2.53; 95% confidence interval, 1.16-5.55). Furthermore, a statistically significant trend associated the C/G + G/G genotypes with the clinical stage of endometriosis. No statistically significant association was observed between AhR codon 554 or ARNT codon 189 polymorphisms and endometriosis.
  
  
  CONCLUSIONS
  AhRR codon 185 polymorphism was associated with susceptibility to and severity of endometriosis in Japanese women.
authors.affiliation: Department of Public Health, Miyazaki Medical College, University of Miyazaki, Miyazaki, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Tsuchiya
authors.forename: Takahiko
authors.initials: T
authors.lastname: Katoh
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Motoyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sasaki
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Tsugane
authors.forename: Tsuyomu
authors.initials: T
authors.lastname: Ikenoue
mesh_terms: D000328:Adult; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000235:genetics*; D051792:Basic Helix-Loop-Helix Proteins; D016022:Case-Control Studies; D016001:Confidence Intervals; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic / Q000235:genetics*; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D012720:Severity of Illness Index
chemical_list: D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D051792:Basic Helix-Loop-Helix Proteins; D018336:Receptors, Aryl Hydrocarbon; D012097:Repressor Proteins; C492162:AHRR protein, human