Construction of a cucumber genetic linkage map with SRAP markers and location of the genes for lateral branch traits.
Using SRAP (sequence-related amplified polymorphism) markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F2 individuals derived from a cross of the two cucumber lines, S06 and S52. In the survey of parental polymorphisms with 182 primer combinations, 64 polymorphism-revealing primer pairs were screened out, which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair. The constructed molecular linkage map included 92 loci, distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci. Based on this linkage map, the quantitative trait loci (QTL) for the lateral branch number (Ibn) and the lateral branch average length (Ibl) in cucumber were identified by QTLMapperl.6. A major QTL Ibn1 located between ME11 SA4B and ME5EM5 in LG2 could explain 10.63% of the total variation with its positively effecting allele from S06. A major QTL Ibl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38% of the total variation with its positively effecting allele from S06.
Wang
Gang
G
Agriculture and Biology School of Shanghai, Jiaotong University, Shanghai 201101, China.
Pan
Junsong
J
Li
Xiaozun
X
He
Huanle
H
Wu
Aizhong
A
Cai
Run
R
D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics*; D018553:Cucumis sativus / Q000235:genetics* / Q000254:growth & development; D017343:Genes, Plant / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D005838:Genotype; D021141:Nucleic Acid Amplification Techniques; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics
D005819:Genetic Markers