title: Serological and molecular diversity in the cattle MHC class I region.
abstract: Information on major histocompatibility complex (MHC) diversity in cattle is important to aid our understanding of immune responses and may contribute to maintenance of healthy cattle populations. Equally, understanding the mechanisms involved in generating this diversity may shed light on the complex nature of mammalian MHC evolution. The aim of this study was to assess molecular and serological variation within cattle MHC class I molecules and to study the mechanisms generating diversity. To address this aim, sequence variation was examined in 12 serologically assigned alleles from three putative loci and correlated with monoclonal antibody (mAb) binding data. The results demonstrate that both alloantisera and mAbs often fail to distinguish gene products that differ by a significant number of amino acids. Conversely, some mAbs could distinguish alleles differing by only one or two amino acids. Examination of the sequences demonstrates sharing of motifs between alleles, some encoded at distinct loci, supporting the occurrence of interlocus recombination within the cattle MHC class I region. The implications of this for MHC sequence diversity, and functional capability, are discussed.
authors.affiliation: Institute for Animal Health, Compton, Newbury, UK. Shirley.ellis@bbsrc.ac.uk
authors.forename: Shirley A
authors.initials: SA
authors.lastname: Ellis
authors.forename: W Ivan
authors.initials: WI
authors.lastname: Morrison
authors.forename: Niall D
authors.initials: ND
authors.lastname: MacHugh
authors.forename: James
authors.initials: J
authors.lastname: Birch
authors.forename: Alison
authors.initials: A
authors.lastname: Burrells
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Stear
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D018076:DNA, Complementary / Q000737:chemistry; D005805:Genes, MHC Class I; D014644:Genetic Variation; D015395:Histocompatibility Antigens Class I / Q000737:chemistry; D008969:Molecular Sequence Data
chemical_list: D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D015395:Histocompatibility Antigens Class I