KDR and Sema3 genes expression in bone marrow stromal cells and hematopoietic cells from leukemia patients and normal individuals.
Kinase domain receptor (KDR) and Semaphorin3 (Sema3) have a functional relationship and are expressed in human bone marrow (BM). We cultured in vitro bone marrow stromal cells (BMSCs) and collected nonadherent cells from patients with acute myeloid leukemia (AML), chronic myelogenous leukemia (CML), acute lymphoblastic leukemia (ALL) and normal individuals. Reverse transcriptase polymerase chain reaction enzyme-linked immunosorbent assay (RT-PCR-ELISA) was performed to examine KDR and Sema3 genes expression, using beta2 microglobulin as an internal reference. KDR expression ratio in normal control BMSCs (97.0%, 32/33) was higher than in its corresponding nonadherent cells (70.8%, 17/24). KDR expression levels in ALL BMSCs and AML nonadherent cells were significantly higher than in normal controls. Sema3 expression ratios in nonadherent cells from AML (78.6%, 11/14) and CML (71.4%, 10/14) were both significantly lower than in normal control (100%, 27/27), its expression levels were also significantly lower than in normal control. Sema3 expression level in normal BMSCs was significantly lower than in nonadherent cells. Sema3 and KDR genes expression levels displayed a significantly positive correlation in normal control and ALL nonadherent cells (r=0.703, P=0.002; r=0.999, P=0.001). These results suggests that KDR may play a critical role in sustaining the hematopoietic microenvironment due to its high expression, and that KDR may be involved in pathogenesis of AML and ALL. Sema3 could also sustain the survival of hematopoietic cells with its high expression, while Sema3 gene expression may be inhibited in AML and CML.
Wang
Zhen
Z
Guangdong Provincial Lung Cancer Research Institute, Cancer Center, Guangdong Provincial People's Hospital, Guangzhou 510800, P.R. China.
Li
Yangqiu
Y
Wu
Xiuli
X
Cheng
Shaohua
S
Yang
Lijian
L
Wu
Yilong
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D002448:Cell Adhesion; D002648:Child; D002675:Child, Preschool; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006412:Hematopoietic Stem Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D040121:Semaphorin-3A / Q000096:biosynthesis* / Q000235:genetics; D017154:Stromal Cells / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis* / Q000235:genetics
C108639:SEMA3A protein, human; D040121:Semaphorin-3A; D040301:Vascular Endothelial Growth Factor Receptor-2