title: Oral vaccination against Helicobacter pylori with recombinant cholera toxin B-subunit.
abstract: BACKGROUND
  The innocuous pure recombinant cholera toxin B-subunit (rCTB) is very attractive as a strong adjuvant for host immunization, but little is known about rCTB's gastric mucosal immunoadjuvanticity against Helicobacter pylori. The immunoadjuvanticity of rCTB against H. pylori was tested.
  
  
  METHODS
  Mice were immunized with sonicated H. pylori and rCTB orally or intranasally and sacrificed on day 42 after immunization. Passive cutaneous anaphylaxis (PCA) test was performed to evaluate IgE-mediated anaphylaxis with serum from mice to which H. pylori-antigen with rCTB had been administered. Immunoglobulin titer specific to H. pylori in serum, lavation of the gastrointestinal tracts and feces were examined. Gastritis in vaccinated mice after a challenge was assessed with the scoring defined from grading of gastric inflammation. H. pylori proliferation after immunization was investigated by counting colony forming units (CFU) per gram of stomach tissue.
  
  
  RESULTS
  PCA test exhibited no reactions against the serum from mice immunized with H. pylori-antigen with rCTB administered orally and intranasally. Oral and nasal coadministrations of rCTB significantly raised systemic and mucosal immunities against H. pylori and suppressed proliferation of H. pylori in gastric mucosa. The score of gastritis in mice immunized orally was significantly higher than that of mice immunized nasally due to postimmunization gastritis. Only oral administration of rCTB suppressed H. pylori proliferation as compared with intranasal administration and without rCTB.
  
  
  CONCLUSIONS
  The present study indicated that rCTB has systemic and mucosal immunoadjuvanticities against H. pylori and that oral vaccination with rCTB might additively support antibiotic eradication.
authors.affiliation: Department of Internal Medicine and Bioregulation, Nagoya City University Graduate School of Medical Sciences, Kawasumi Mizuho, Nagoya 467-8601, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Kubota
authors.forename: Takashi
authors.initials: T
authors.lastname: Joh
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tanida
authors.forename: Makoto
authors.initials: M
authors.lastname: Sasaki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kataoka
authors.forename: Katsushi
authors.initials: K
authors.lastname: Watanabe
authors.forename: Keisuke
authors.initials: K
authors.lastname: Itoh
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Oshima
authors.forename: Naotaka
authors.initials: N
authors.lastname: Ogasawara
authors.forename: Shouzo
authors.initials: S
authors.lastname: Togawa
authors.forename: Tsuneya
authors.initials: T
authors.lastname: Wada
authors.forename: Tomonori
authors.initials: T
authors.lastname: Yamada
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Mori
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Fujita
authors.forename: Takaya
authors.initials: T
authors.lastname: Shimura
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Ohara
authors.forename: Masanori
authors.initials: M
authors.lastname: Isaka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yasuda
authors.forename: Makoto
authors.initials: M
authors.lastname: Itoh
mesh_terms: D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002772:Cholera Toxin / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D015169:Colony Count, Microbial; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology; D016481:Helicobacter Infections / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D016480:Helicobacter pylori / Q000254:growth & development / Q000472:pathogenicity*; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D011994:Recombinant Proteins; D002772:Cholera Toxin