Lycopene-epsilon-cyclase pre-mRNA is alternatively spliced in Cara Cara navel orange (Citrus sinensis Osbeck).
Lycopene-epsilon-cyclase is one of the key enzymes related to alpha-carotene metabolism in plants. A full-length cDNA of 1300 bp encoding lycopene-epsilon-cyclase (Lyce) was generated from Cara Cara navel orange, a unique navel orange containing both lycopene and beta-carotene in its pulp, with little or no alpha-carotene. The gene had a 14 bp nucleotides deletion and caused a terminal mutation. DNA sequence corresponding to the deletion region revealed that two repeats of 6 bp (AGGTGT) were flanking the region in both Cara Cara and its original variety, Washington navel oranges, but a 2 bp (AT) insertion was only found in Cara Cara which explain the alternative splicing character of the gene.
Tao
Neng-Guo
NG
National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, 430070, Wuhan, P.R. China.
Xu
Juan
J
Cheng
Yun-Jiang
YJ
Deng
Xiu-xin
XX
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D032084:Citrus sinensis / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D019753:Intramolecular Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics; D012322:RNA Precursors / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
D018076:DNA, Complementary; D010940:Plant Proteins; D012322:RNA Precursors; D019753:Intramolecular Lyases; C070925:lycopene cyclase-isomerase