[Study on low-temperature alkaline lipase from Bohaisea-9145].
Lipase-overproducing marine yeast Bohaisea-9145 isolated from the Bohai sea region was identified as psychrotrophlic Yarrowia lipolytica. The optimum composition of culture medium was ground soybean 4%, rice bran 4%, peanut power 4%, crude peanut oil 0.5%, MgSO4 0.05%, KH2PPO4 0.2%. The optimum temperature and pH were 26 +/- 1 degrees C pH5.0, the time of fermentation cycle was 23h. The lipase activity was improved to 258.67 U/mL through optimization, four-fold more than that of initial strain. The lipase displayed maximum activity at pH 8.5, 35 degrees C and was stable within the range of pH 4.0 - 9.0 at low temperature. It had high thermosensitivity, good compatibility with familiar metal ions and chemical reagents. It was antioxidant and had strong resistance to high salinity. As a novel marine low-temperature lipase, it has promising prospect in many fields, especially as an additive of detergent.
Shao
Tie-Juan
TJ
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. stjouqd-2001@sohu.com
Sun
Mi
M
Zheng
Jia-Sheng
JS
Wang
Yue-Jun
YJ
Qiu
Xiu-Bao
XB
D008049:Lipase / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013696:Temperature; D014871:Water Microbiology; D025062:Yarrowia / Q000201:enzymology* / Q000235:genetics
D008049:Lipase