title: Proteome analysis reveals adaptation of Pseudomonas aeruginosa to the cystic fibrosis lung environment.
abstract: Pseudomonas aeruginosa is known for the chronic lung colonization of cystic fibrosis (CF) patients in addition to eye, ear and urinary tract infections. With the underlying disease CF patients are predisposed to P. aeruginosa chronic lung infection, which leads to morbidity and mortality. In this study, we compared the protein expression profile of a CF lung-adapted P. aeruginosa strain C with that of the burn-wound isolate PAO. Differentially expressed proteins from the whole-cell, membrane, periplasmic as well as extracellular fraction were identified. The whole-cell proteome of strain C showed down-regulation of several proteins involved in amino acid metabolism, fatty acid metabolism, energy metabolism and adaptation leading to a highly distinct proteome pattern for strain C in comparison to PAO. Analysis of secreted proteins by strain C compared to PAO revealed differential expression of virulence factors under non-inducing conditions. The membrane proteome of strain C showed modulation of the expression of porins involved in nutrient and antibiotic influx. The proteome of the periplasmic space of strain C showed retention of elastase despite that the equal amounts were secreted by strain C and PAO. Altogether, our results elucidate adaptive strategies of P. aeruginosa towards the nutrient-rich CF lung habitat during the course of chronic colonization.
authors.affiliation: Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Dinesh Diraviam
authors.initials: DD
authors.lastname: Sriramulu
authors.forename: Manfred
authors.initials: M
authors.lastname: Nimtz
authors.forename: Ute
authors.initials: U
authors.lastname: Romling
mesh_terms: D000222:Adaptation, Physiological; D003550:Cystic Fibrosis / Q000382:microbiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D020543:Proteome; D011550:Pseudomonas aeruginosa / Q000378:metabolism* / Q000502:physiology
chemical_list: D020543:Proteome