title: Glucose transporter GLUT1 in colorectal adenocarcinoma cell lines is inversely correlated with tumour cell proliferation.
abstract: The high glucose consumption of tumour cells has been well known since Warburg's investigations. Nevertheless, the reason for this phenomenon is still a matter of speculation. Glucose uptake of tumour cells is mainly mediated by GLUT1. In this study, the question of whether glucose uptake as well as expression of GLUT1 are both related to tumour cell proliferation was addressed. Three kinetically different cell lines of colorectal adenocarcinomas (HRT-18, HT29 and CX-2) were used for the experiments. All the cell lines expressed GLUT1 at a high level, being mainly localized within the cell membrane and the endoplasmic reticulum. Surprisingly, the highest expression of GLUT1 was found in the most slowly proliferating cell line, CX-2. Moreover, induction of cell cycle arrest increased both GLUT1 expression and glucose consumption, as well as global protein synthesis. These data suggest that the protein synthesis of tumour cells is much more glucose-consuming than proliferation and that both processes are inversely related.
authors.affiliation: Institute of Pathology, Martin-Luther-University Halle-Wittenberg, Magdeburger Str. 14, D-06097 Halle (Saale), Germany. steffen.hauptmann@medizin.uni-halle.de
authors.forename: Steffen
authors.initials: S
authors.lastname: Hauptmann
authors.forename: Verena
authors.initials: V
authors.lastname: Grünewald
authors.forename: Denise
authors.initials: D
authors.lastname: Molls
authors.forename: Wolfgang D
authors.initials: WD
authors.lastname: Schmitt
authors.forename: Martin
authors.initials: M
authors.lastname: Köbel
authors.forename: Karen
authors.initials: K
authors.lastname: Kriese
authors.forename: Annette
authors.initials: A
authors.lastname: Schürmann
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology*; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D048708:Cell Growth Processes / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1; D019073:HT29 Cells; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D000964:Antimetabolites, Antineoplastic; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; C495677:SLC2A1 protein, human; D005947:Glucose; D008727:Methotrexate