title: [Effect of wortmannin on endothelial cell proliferation and migration induced by high glucose Müller cell conditioned medium].
abstract: OBJECTIVE
  To investigate the effect of wortmannin on endothelial cells proliferation and migration induced by high glucose Müller cell conditioned medium (HGMCM).
  
  
  METHODS
  Immunofluorescence, flow cytometry and Boyden chamber migration models were used to analyze the effect of wortmannin on endothelial cells.
  
  
  RESULTS
  50 nmol/L wortmannin could significantly inhibit the proliferation and migration of endothelial cells induced by HGMCM. The percentage of endothelial cells in S phase was obviously decreased [from (37.82 +/- 0.57)% to (21.91 +/- 0.23)%, P < 0.01], while there was an increase in the percentage of cells in G(0)/G(1) phase [from (54.57 +/- 1.19)% to (65.59 +/- 0.41)%, < 0.01] and G(2)/M phase (< 0.05).
  
  
  CONCLUSIONS
  Wortmannin can inhibit proliferation and migration of endothelial cells induced by HGMCM, suggesting that wortmannin carries an anti-angiogenetic ability in diseased retina.
authors.affiliation: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China.
authors.forename: Yue-hui
authors.initials: YH
authors.lastname: Wang
authors.forename: Xi-yuan
authors.initials: XY
authors.lastname: Wang
authors.forename: Xin-rui
authors.initials: XR
authors.lastname: Wang
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Ni
authors.forename: Ying
authors.initials: Y
authors.lastname: Xin
authors.forename: Bo
authors.initials: B
authors.lastname: Shi
authors.forename: Jia-xiang
authors.initials: JX
authors.lastname: Wu
mesh_terms: D000730:Androstadienes / Q000494:pharmacology*; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D005947:Glucose; D006801:Humans; D008297:Male; D009389:Neovascularization, Pathologic; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011817:Rabbits; D012160:Retina / Q000166:cytology*; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D017077:Culture Media, Conditioned; D000081082:Phosphoinositide-3 Kinase Inhibitors; D005947:Glucose; D000077191:Wortmannin