title: Protective effect of vascular endothelial growth factor on histologic changes in testicular ischemia-reperfusion injury.
abstract: OBJECTIVE
  To evaluate the efficacy of vascular endothelial growth factor (VEGF) on long-term histologic damage in testicular ischemia-reperfusion injury.
  
  
  METHODS
  Controlled experimental study using rats.
  
  
  METHODS
  University of Mersin School of Medicine, Mersin, Turkey.
  
  
  METHODS
  Sixteen adult male Wistar rats.
  
  
  METHODS
  Five rats underwent 2 hours of testicular torsion. Six rats received VEGF injection into the testis before detorsion. Five rats underwent sham operation.
  
  
  METHODS
  Mean seminiferous tubular diameter (MSTD), germinal epithelial cell thickness (GECT), mean testicular biopsy score (MTBS), and apoptosis (caspase-3-positive cells).
  
  
  RESULTS
  Testicular torsion-detorsion caused a significant decrease in MSTD, GECT, and MTBS and a significant increase in the mean value of caspase-3-positive cells in ipsilateral testes but not in the contralateral testes. The MSTD, GECT, and MTBS of the ipsilateral testes were significantly higher in the group treated with VEGF than in the torsion-detorsion group. The animals treated with VEGF had a significant decrease in the mean value of ipsilateral testicular caspase-3-positive cells compared with the torsion-detorsion group.
  
  
  CONCLUSIONS
  Vascular endothelial growth factor might have a role in testicular damage caused by ischemia-reperfusion. Administering VEGF before reperfusion might have the potential to decrease the long-term histologic damage after testicular torsion.
authors.affiliation: Department of Urology, University of Mersin School of Medicine, Mersin, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Tunçkiran
authors.forename: Selahittin
authors.initials: S
authors.lastname: Cayan
authors.forename: Murat
authors.initials: M
authors.lastname: Bozlu
authors.forename: Necat
authors.initials: N
authors.lastname: Yilmaz
authors.forename: Deniz
authors.initials: D
authors.lastname: Acar
authors.forename: Erdem
authors.initials: E
authors.lastname: Akbay
mesh_terms: D000818:Animals; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology* / Q000628:therapy; D013086:Spermatic Cord Torsion / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology / Q000517:prevention & control; D013737:Testis / Q000098:blood supply / Q000473:pathology* / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage / Q000502:physiology*
chemical_list: D042461:Vascular Endothelial Growth Factor A