Effect of short- vs. long-term estrogen on reinstatement of cocaine-seeking behavior in female rats.
Estrogen effects on cocaine-induced reinstatement of lever responding were examined in sham-operated, vehicle-treated (SH+VEH), ovariectomized (OVX+VEH), and OVX female Wistar rats with estrogen replacement (OVX+EB). The effect of long- (64+/-1.56 days) and short-term (9 days) EB treatment on reinstatement of cocaine-seeking behavior was compared in Experiment 1 and 2, respectively, in order to compare the effect of EB when it was present during the development vs. expression of reinstatement of cocaine-seeking behavior. Rats were trained to self-administer 0.4 mg/kg/inf cocaine. After the acquisition criteria were met, rats continued to respond for cocaine for 2 h/day for a 14-day maintenance period. Cocaine was then replaced with saline and the 21-day extinction period commenced. Subsequently, rats were tested for reinstatement of lever responding on the previously drug-paired lever after alternating daily injections of saline or cocaine. In both experiments, there were no differences between groups in self-administration behavior during training, maintenance, or extinction. In Experiment 1, SH+VEH and chronically treated OVX+EB rats had greater cocaine-induced reinstatement than OVX+VEH rats. In Experiment 2, short-term treated OVX+EB rats also showed enhanced cocaine-induced reinstatement compared to OVX+VEH rats. The results indicate that EB-mediated enhancement of cocaine-induced reinstatement is dependent on EB presence during the expression of reinstatement but not during the formation of stimulus-reward associations during the development of cocaine-reinforced behavior.
Larson
Erin B
EB
Graduate Program in Neuroscience, University of Minnesota, MMC 392 Minneapolis, MN 55455, USA. larso323@umn.edu
Roth
Megan E
ME
Anker
Justin J
JJ
Carroll
Marilyn E
ME
D000818:Animals; D003042:Cocaine / Q000008:administration & dosage*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D009042:Motivation; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar
D004967:Estrogens; D003042:Cocaine