title: Recurrent duplication of Xq27-qter in hematological malignancies revealed by multicolor fluorescence in situ hybridization and multicolor banding.
abstract: Multicolor fluorescence in situ hybridization (M-FISH) experiments were performed to determine the composition of abnormal complex karyotypes in 15 cases of hematological malignancy. Four cases were found to have unsuspected unbalanced X chromosome translocations, which resulted in the presence of extra X chromosome material. We determined the identity of the duplicated chromosome regions using the multicolor banding (mBAND) technique. Xq27-qter was duplicated in three of the four male cases with an X chromosome abnormality (i.e., in one third of male cases and one fifth of all cases). These preliminary results may point to the existence of a recurrent chromosome abnormality, either translocation at a specific Xq27 locus or duplication of Xq27-qter.
authors.affiliation: University of Melbourne Department of Medicine, St Vincent's Hospital, Melbourne, Victoria, Australia. ruth.mackinnon@svhm.org.au
authors.forename: Ruth N
authors.initials: RN
authors.lastname: MacKinnon
authors.forename: Adrian
authors.initials: A
authors.lastname: Zordan
authors.forename: Lynda J
authors.initials: LJ
authors.lastname: Campbell
mesh_terms: D000368:Aged; D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods*; D020223:Chromosome Painting; D041321:Chromosomes, Human, X; D019337:Hematologic Neoplasms / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged