title: Maturation of Borna disease virus glycoprotein.
abstract: The maturation of Borna disease virus (BDV) glycoprotein GP was studied in regard to intracellular compartmentalization, compartmentalization signal-domains, proteolytic processing, and packaging into virus particles. Our data show that BDV-GP is (i) predominantly located in the endoplasmic reticulum (ER), (ii) partially exists in the ER already as cleaved subunits GP-N and GP-C, (iii) is directed to the ER/cis-Golgi region by its transmembrane and/or cytoplasmic domains in CD8-BDV-GP hybrid constructs and (iv) is incorporated in the virus particles as authentic BDV glycoprotein exclusively in the cleaved form decorated with N-glycans of the complex type. Downregulation of BDV-glycoproteins on the cell surface, their limited proteolytic processing, and protection of antigenic epitopes on the viral glycoproteins by host-identical N-glycans are different strategies for persistent virus infections.
authors.affiliation: Institut für Virologie der Philipps-Universität Marburg, Robert-Koch-Strasse 17, D-35037 Marburg, Germany. eickmann@staff.uni-marburg.de
authors.forename: Markus
authors.initials: M
authors.lastname: Eickmann
authors.forename: Simone
authors.initials: S
authors.lastname: Kiermayer
authors.forename: Ina
authors.initials: I
authors.lastname: Kraus
authors.forename: Melanie
authors.initials: M
authors.lastname: Gössl
authors.forename: Jürgen A
authors.initials: JA
authors.lastname: Richt
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Garten
mesh_terms: D000818:Animals; D001891:Borna disease virus / Q000378:metabolism*; D019556:COS Cells; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum / Q000378:metabolism; D000939:Epitopes; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000939:Epitopes; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins