Changes in gene expression of Zif, c-fos and cyclooxygenase-2 associated with spinal long-term potentiation.
Spinal cord long-term potentiation is often studied as a model for cellular memory of nociceptive information. In the present report, extracellular single-unit recordings and quantitative real-time reverse transcriptase polymerase chain reaction were used to examine whether the induction of spinal cord long-term potentiation involves changes in expression of Zif, c-fos and cyclooxygenase-2. The data demonstrated that induction of spinal cord long-term potentiation was associated with a transient increase in the expression of Zif at 120 min (p < 0.05, long-term potentiation group vs. control group). In contrast, a decrease or no changes were observed in the expression of c-fos and cyclooxygenase-2. The transient increase of the expression of Zif is consistent with an involvement in the transition from the early to the late-phase of spinal cord long-term potentiation.
Gjerstad
Johannes
J
National Institute of Occupational Health, Pb 8149 Dep, N-0033 Oslo, Norway. johannes.gjerstad@stami.no
Lien
Guro F
GF
Pedersen
Linda M
LM
Valen
Elin C
EC
Mollerup
Steen
S
D000818:Animals; D051546:Cyclooxygenase 2; D004268:DNA-Binding Proteins / Q000235:genetics*; D051766:Early Growth Response Protein 1; D004594:Electrophysiology; D005260:Female; D015870:Gene Expression / Q000502:physiology; D017781:Genes, Immediate-Early / Q000502:physiology; D017874:Immediate-Early Proteins / Q000235:genetics*; D017774:Long-Term Potentiation / Q000502:physiology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D014157:Transcription Factors; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases