[Expression of caspase 3 and p53 during physiological apoptosis and apoptosis induced by three teratologic agents during early craniofacial development of the mouse embryo].
The neural crest-derived mesectoderm gives rise to physiologic apoptosis areas in early vertebrate embryos. Certain teratologic agents increase this phenomenon. The purpose of this work was to detect caspase 3 (which is associated with the apoptosis cascade) and p53 in cell death areas, both during physiological apoptosis and during apoptosis induced by three agents (retinoic acid, methyl-triazene, irradiation). Antibody revelation was performed using the aBC peroxidase kit. Quantifications were also performed on histological sections. We observed caspase 3 uptake on some apoptotic and preapoptotic cells in control embryos, and in the embryos exposed to the three teratogens. Immunoreactivity generally preceded the development of cytological features of apoptosis. However, p53 was expressed only in the embryos exposed to ionizing radiation and methyl-triazene (an alkylating agent), but not significantly in embryos exposed to retinoic acid. The present results throw some light on apoptosis mechanisms in several teratologic conditions.
Gashegu
J
J
Laboratoire d'Anatomie et Embryologie, Faculté de Médecine, Universite Libre de Bruxelles, CP 619, route de Lennik 808, B 1070 Bruxelles.
Vanmuylder
N
N
Kassengera
Z
Z
Evrard
L
L
Glineur
R
R
Philippson
C
C
Rooze
M
M
Louryan
S
S
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D053148:Caspase 3; D020169:Caspases / Q000235:genetics* / Q000378:metabolism; D047108:Embryonic Development; D005145:Face / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000528:radiation effects; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D009432:Neural Crest / Q000166:cytology / Q000502:physiology; D012886:Skull / Q000166:cytology / Q000196:embryology* / Q000528:radiation effects; D013723:Teratogens / Q000633:toxicity*; D014212:Tretinoin / Q000494:pharmacology; D014226:Triazenes / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D013723:Teratogens; D014226:Triazenes; D016159:Tumor Suppressor Protein p53; D014212:Tretinoin; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases