Mitostatic and mitodepressive activities of polysaccharides from Saccharomyces cerevisiae Meyen T-411 strain and their preliminary chemical studies.
Hot water extracts of Saccharomyces cerevisiae Meyen T-411 strain provided the A and B polysaccharide fractions by alcohol precipitation. Both fractions revealed marked mitostatic and, or mitodepressive activities in Allium test. The fractions were purified with DEAE-Sephadex A-25 column what resulted in obtaining of the A1-A5 and the B1-B5 subfractions. For the fractions and subfractions the elemental, sugar and amino acid analyses were carried out. Weight averaged molecular weights Mw of the A1 and B1 subfractions were estimated. Purified the A1-A5 and B1-B5 subfractions were again evaluated in Allium test but their activities markedly declined.
Grzybek
J
J
Department of Pharmaceutical Botany, School of Medicine, 16 Krupnicza Str., 31-123 Kraków, Poland.
Szafranek
J
J
Kaczmarek
J
J
Gajdus
J
J
Czaja
M
M
D008938:Mitosis / Q000187:drug effects*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
D011134:Polysaccharides