title: Analysis of 8-hydroxydeoxyguanosine among workers exposed to diesel particulate exhaust: comparison with urinary metabolites and PAH air monitoring.
abstract: Oxidative DNA damage and repair, as measured by 8-hydroxy-2'-deoxyguanosine (8-OHdG) in urine and DNA samples were studied in association with work-related diesel exhaust exposure among garage and waste collection workers. Seasonal variations of the urinary 8-OHdG levels in pre- and two post-workshift urine samples of 29 exposed workers and 36 control persons were evaluated. The mean+/-SE levels of post-workshift 8-OHdG (mumol/mol crea) were 1.52+/-0.44 in winter and 1.61+/-0.33 in summer for the exposed workers, and 1.56+/-0.61 in winter and 1.43+/-0.49 in summer for the controls, respectively. No significant difference in the urinary 8-OHdG levels between exposed workers and control subjects in winter (p=0.923) and summer (p=0.350) was observed. A linear mixed model, adjusted for years of employment, age, ex/non-smoking and BMI, indicated no significant dose exposure-relationships between the urinary 8-OHdG and 15 PAH air concentrations nor between the 8-OHdG and 7 PAH monohydroxy-metabolites analyzed in the same workers. 8-OHdG was also analyzed in the mononuclear cell DNA of 19 exposed and 18 control subjects. The mean value of 8-OHdG/non-modified 2'-deoxyguanosine (8-OHdG/105 dG+/-SE) were 4.89+/-0.17 for the exposed and 4.11+/-0.16 for the control persons, which showed no correlation with the urinary 8-OHdG levels (r=0.01, n=28, P=0.96). The PAH exposure at workplaces was mainly composed of volatile compounds, particularly naphthalene, suggesting low exposure through the respiratory tract and a low effect of PAH in ROS induction.
authors.affiliation: Finnish Institute of Occupational Health, Helsinki, Finland.
authors.forename: Minna
authors.initials: M
authors.lastname: Harri
authors.forename: Peter
authors.initials: P
authors.lastname: Svoboda
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Mori
authors.forename: Pertti
authors.initials: P
authors.lastname: Mutanen
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kasai
authors.forename: Kirsti
authors.initials: K
authors.lastname: Savela
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000395:Air Pollutants, Occupational / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D006801:Humans; D019570:Inhalation Exposure; D008214:Lymphocytes / Q000737:chemistry; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry; D016273:Occupational Exposure / Q000032:analysis*; D018384:Oxidative Stress; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000652:urine; D012621:Seasons; D001335:Vehicle Emissions / Q000032:analysis*
chemical_list: D000395:Air Pollutants, Occupational; D011084:Polycyclic Aromatic Hydrocarbons; D001335:Vehicle Emissions; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003404:Creatinine; D003849:Deoxyguanosine