[Study on tissue culture and plant regeneration of Nervilia fordii].
OBJECTIVE
Tissue culture of Nervilia fordii to get its regeneration.


METHODS
Effects on indusement of rhizoma and plant regeneration of different implants, density of hormones, additives were studied.


RESULTS
The best implant was conn. Effect of 6-BA 2 mg x L(-1) were better than 6-BA 1 on rhizoma reducing. The coconut juice and active carbon could increase the growth of rhizoma.


CONCLUSIONS
Bud could be induced on 1/2MS + 6-BA 2 mg x L(-1) by inoculating corm on culture mediem, and could grow lots of rhizoma after inoculating on the culture mediem containing 10% coconut juice and 1 per thousand active carbon. The white rhizoma could be induced to corms and regeneration plants on 1/2MS + 1 per thousand active carbon. The green rhizoma could be induced directerly to regeneration plants on 1/2MS + 6-BA 2 + NAA 2.
Du
Qin
Q
School of Chinese Materia Medica, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Chen
Wen-Li
WL
Wang
Zhen-Hua
ZH
D002244:Carbon / Q000494:pharmacology; D003470:Culture Media; D029595:Orchidaceae / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010946:Plants, Medicinal / Q000254:growth & development*; D012038:Regeneration; D027343:Rhizome / Q000254:growth & development; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010937:Plant Growth Regulators; D002244:Carbon