title: The duality of beta-catenin function: a requirement in lens morphogenesis and signaling suppression of lens fate in periocular ectoderm.
abstract: In the current analysis, we have investigated both the cytoskeletal and signaling roles of beta-catenin during the early phases of lens development using conditional loss- and gain-of-function strategies. Conditional loss of beta-catenin in the presumptive lens does not perturb the normal sequential appearance of lens fate markers but results in a dramatic failure of the coordinated epithelial cell behavior that constitutes lens morphogenesis. Similarly, loss-of-function for Lrp6, the Wnt pathway coreceptor expressed in the eye primordium, does not prevent expression of lens induction markers. Surprisingly, conditional deletion of beta-catenin in periocular ectoderm results in the formation of Prox-1 and beta-crystallin-positive ectopic lentoid bodies. Combined with the observation that the Wnt pathway reporter TOPGAL is expressed in nasal periocular ectoderm, these data suggest that, in this location, the canonical Wnt signaling pathway normally suppresses lens fate in favor of other structures. Consistent with this proposal, a dominant-active form of beta-catenin causes a loss of lens fate and a complete absence of lens development when expressed in the presumptive lens ectoderm.
authors.affiliation: Division of Developmental Biology, Department of Ophthalmology, Children's Hospital Research Foundation, 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
authors.forename: April N
authors.initials: AN
authors.lastname: Smith
authors.forename: Leigh-Anne D
authors.initials: LA
authors.lastname: Miller
authors.forename: Ni
authors.initials: N
authors.lastname: Song
authors.forename: M Mark
authors.initials: MM
authors.lastname: Taketo
authors.forename: Richard A
authors.initials: RA
authors.lastname: Lang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D003599:Cytoskeleton / Q000378:metabolism; D004475:Ectoderm / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D005697:Galactosides; D007150:Immunohistochemistry; D007211:Indoles; D026502:LDL-Receptor Related Proteins / Q000378:metabolism; D007908:Lens, Crystalline / Q000196:embryology*; D060488:Low Density Lipoprotein Receptor-Related Protein-6; D051379:Mice; D008822:Mice, Transgenic; D009024:Morphogenesis / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D005697:Galactosides; D007211:Indoles; D026502:LDL-Receptor Related Proteins; D060488:Low Density Lipoprotein Receptor-Related Protein-6; C555476:Lrp6 protein, mouse; D051176:beta Catenin; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside