title: Simultaneous measurement of superoxide generation and intracellular calcium ion of neutrophil-like culture cells.
abstract: We have developed a novel method for simultaneously measuring fluorescence and chemiluminescence. The generation of superoxide anion and the intracellular Ca(2+) ion concentration of neutrophil-like cells stimulated by agonists were measured in real time by our method. Our results were in agreement with the intracellular signalling in the neutrophils. We also found that the presence of Zn(2+) ion inhibited both the generation of superoxide anion and the influx of Ca(2+) ions.
authors.affiliation: Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamamatsu-City 434-8601, Japan. satozono@crl.hpk.co.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Satozono
authors.forename: Kimiko
authors.initials: K
authors.lastname: Kazumura
authors.forename: Sigetoshi
authors.initials: S
authors.lastname: Okazaki
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Hiramatsu
mesh_terms: D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D012680:Sensitivity and Specificity; D015398:Signal Transduction / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000032:analysis / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000494:pharmacology
chemical_list: D013481:Superoxides; C016658:phorbolol myristate acetate; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015032:Zinc; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium