Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli.
A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg(-1) dry cells. Recombinant allophycocyanin significantly inhibited H22 hepatoma (p ( 0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg(-1) d(-1).
Ge
Baosheng
B
Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, P.R. China.
Tang
Zhihong
Z
Lin
Liping
L
Ren
Yuhong
Y
Yang
Yu
Y
Qin
Song
S
D000818:Animals; D019149:Bioreactors / Q000382:microbiology; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000517:prevention & control; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005260:Female; D005285:Fermentation; D015870:Gene Expression; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000517:prevention & control; D051379:Mice; D009368:Neoplasm Transplantation; D010798:Phycocyanin / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology
D011994:Recombinant Proteins; C002497:allophycocyanin; D010798:Phycocyanin