title: [Nine polymorphisms of fibrinogen gene and their association with plasma fibrinogen levels in Hainan Han population].
abstract: OBJECTIVE
  To investigate the allelic frequencies of polymorphisms of alpha Taq I and beta Bcl I, Hinf I A/C, 448 G/A, beta BsmA I G/C, +1689T/G, -148C/T, -249C/T, -455G/A in Hainan Han population and their association with plasma fibrinogen level.
  
  
  METHODS
  Turbidmetric assay was used to measure plasma fibrinogen level of two hundred and thirty-eight healthy individuals. The genotypes were characterized by PCR-RFLP and sequence analysis. The relationships between the genotypes and plasma fibrinogen levels were analyzed by t test and ANOVA.
  
  
  RESULTS
  The frequencies of the rare alleles of alpha Taq I and beta Bcl I, Hinf I A/C, 448 G/A, beta BsmA I G/C, +1689T/G, -148C/T, -249C/T, -455G/A polymorphisms were 0.445, 0.239, 0.134, 0.235, 0.273, 0.241, 0.265, 0.441, 0.254 respectively. In the general population, the plasma fibrinogen level is significantly higher in the groups of genotypes -455GA and AA, -148CT and TT, alpha Taq I T1T1 than in the group of wild types(P=0.004, 0.015 and 0.043 respectively). In the men, plasma fibrinogen level is significantly higher in the groups of genotypes -455GA and AA, -148CT and TT, alpha Taq I T1T1, alpha Taq I T1T2 than in the group of wild types(P=0.001, 0.023, 0.003 and 0.032 respectively). In the women, no significant genotype association with plasma fibrinogen level was detected.
  
  
  CONCLUSIONS
  There was linkage disequilibrium between the fibrinogen gene loci. The beta -455G/A beta 448G/A, alpha Fg Taq I polymorphisms were associated with the difference in plasma fibrinogen in men. A(-455), T(-148) and alpha Taq I T1 alleles were associated with higher fibrinogen levels.
authors.affiliation: Department of Biochemistry, Hainan Medical College, Haikou, Hainan, 571101 PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Liang
authors.forename: Chuan
authors.initials: C
authors.lastname: Sun
authors.forename: Feng
authors.initials: F
authors.lastname: Xiao
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Tang
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Chen
authors.forename: Dai-feng
authors.initials: DF
authors.lastname: Zhou
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Guo-xun
authors.initials: GX
authors.lastname: Liu
authors.forename: Shuang
authors.initials: S
authors.lastname: Cheng
authors.forename: Guang-xin
authors.initials: GX
authors.lastname: Ling
authors.forename: Cheng-xin
authors.initials: CX
authors.lastname: Bao
authors.forename: Wang-wei
authors.initials: WW
authors.lastname: Cai
mesh_terms: D000328:Adult; D002681:China; D005260:Female; D005340:Fibrinogen / Q000235:genetics* / Q000378:metabolism; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D005340:Fibrinogen