Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China.
Glyphosate has been used globally as a safe herbicide for weed control. It inhibits 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (AroA), which is a key enzyme in the aromatic amino acid biosynthetic pathway in microorganisms and plants. A Pseudomonas putida strain, 4G-1, was isolated from a soil heavily contaminated by glyphosate in China. Its AroA-encoding gene (aroA) has been cloned, sequenced, and expressed in Escherichia coli. Phylogenetic analysis revealed that this AroA belongs neither to class I nor to class II AroA enzymes. When compared with E. coli AroA, 4G-1 AroA shows similar values for K(m)[PEP], K(m)[S3P], and specific enzyme activity. Moreover, 4G-1 AroA exhibits high tolerance to glyphosate, which indicates a protein with a high potential for structural and functional studies of AroA in general and its potential usage for the generation of transgenic crops resistant to the herbicide.
Sun
Yi-Cheng
YC
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100081, People's Republic of China.
Chen
Yan-Cheng
YC
Tian
Zhe-Xian
ZX
Li
Feng-Mei
FM
Wang
Xin-Yue
XY
Zhang
Jing
J
Xiao
Zhen-Long
ZL
Lin
Min
M
Gilmartin
Niamh
N
Dowling
David N
DN
Wang
Yi-Ping
YP
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D002681:China; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016958:Pseudomonas putida / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000494:pharmacology; D000097797:Glyphosate
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases; D005998:Glycine; D006540:Herbicides; D012989:Soil Pollutants; D000097797:Glyphosate