De-repression and comparison of oil-water separation activity of the dibenzothiophene desulfurizing bacterium, Mycobacterium sp. G3.
Expression of the desulfurization genes (dsz) in Mycobacterium sp. G3 is repressed by sulfate, which is the product of biodesulfurization. An expression clone, pSMTABC, was constructed by placing the dsz genes downstream of the hsp60 promoter and the constructed plasmid was electroporated into G3. The recombinant strain G3-1 desulfurized dibenzothiophene in the presence of 0.5 mM: sulfate while the Dsz phenotype was completely repressed in the wild-type strain. However, there was no significant increase in the amount of desulfurization enzymes in G3-1. In addition, G3 had superior separation of diesel oil-water separation activity compared to E. coli, which is superior to desulfurizing rhodococci.
Takada
Masaki
M
Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki, Japan.
Nomura
Nobuhiko
N
Okada
Hideki
H
Nakajima-Kambe
Toshiaki
T
Nakahara
Tadaatsu
T
Uchiyama
Hiroo
H
D001673:Biodegradation, Environmental; D009161:Mycobacterium / Q000235:genetics / Q000378:metabolism*; D009821:Oils / Q000737:chemistry; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*; D014169:Transformation, Bacterial; D014867:Water / Q000737:chemistry
D009821:Oils; D011993:Recombinant Fusion Proteins; D013876:Thiophenes; D014867:Water; D013455:Sulfur; D010105:Oxygenases; C421210:dibenzothiophene sulfone monooxygenase; C016366:dibenzothiophene