[Tissue culture and plantlet regeneration from embryo of Saussurea involucrate].
OBJECTIVE
To establish a protocol of rapid clonal propagation of Saussurea involucrate by using embryo as explants.


METHODS
MS medium was used as basal medium and BA and NAA were supplemented to find out the optimal hormone combinations for adventitious buds initiation, adventitious bud multiplication and rooting.


RESULTS
All embryo explants started to grow adventitious buds within 45 days when they were cultured on the media supplemented with 0.02-0.1 mg x L(-1) NAA and 2 mg x L(-1) BA; The adventitious buds multiplicated within 30 days when they were transferred to the media containing 0.1-0.2 mg x L(-1) NAA and 2-3 mg x L(-1) BA; 92% of the adventitious buds rooted well after they were planted on the MS medium containing macroelements at half strength and 0.4 mg x L(-1) NAA for 25 days. The regenerated plantlets grew well after they were transplanted and the survival rate was up to 70%.


CONCLUSIONS
Plantlets of S. invducrate regenerated high frequence through adventitious bud.
Wu
Li-Qin
LQ
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
Guo
Shun-Xing
SX
Xiao
Pei-Gen
PG
D003470:Culture Media; D010937:Plant Growth Regulators / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D012038:Regeneration; D030441:Saussurea / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D010937:Plant Growth Regulators