SMYD3-NY, a novel SMYD3 mRNA transcript variant, may have a role in human spermatogenesis.
Identification of genes specifically expressed in adult and fetal testis is important to further our understanding of testis development and function. In this study, a novel SMYD3 transcript variant, termed SMYD3-NY (GenBank Accession No. AY186742), was identified by hybridization of adult and fetal human testis cDNA probes with a human cDNA microarray. SMYD3-NY transcript was expressed at 2.3-fold higher levels in adult human testis than in fetal testis, with a low expression level in human spermatozoa. Bioinformatical analysis showed that SMYD3-NY protein has the SET domain that is involved in histone methyltransferase (HTMase) activity. Southern blotting showed that SMYD3-NY is distributed in several tissues, including testis. In summary, SMYD3-NY is a novel transcript variant of the SMYD3 gene, and SMYD3-NY protein may influence transcriptional regulation during spermatogenesis via HTMase activity.
Zhou
Zuomin
Z
Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
Ren
Xuan
X
Huang
Xiaoyan
X
Lu
Li
L
Xu
Min
M
Yin
LanLan
L
Li
Jianmin
J
Sha
Jiahao
J
D000328:Adult; D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D020869:Gene Expression Profiling; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017385:Sequence Homology; D013091:Spermatogenesis / Q000235:genetics* / Q000502:physiology*; D013737:Testis / Q000166:cytology
D012333:RNA, Messenger; D011495:Histone-Lysine N-Methyltransferase; C506278:SMYD3 protein, human