title: Development of a one-step strip test for the diagnosis of chicken infectious bursal disease.
abstract: A rapid diagnostic strip for chicken infectious bursal disease (IBD) was developed based on membrane chromatography using high-affinity monoclonal antibodies directed to chicken infectious bursal disease virus (IBDV). The diagnostic strip has high specificity for detection of chicken IBDV antigen and recognizes a variety of the virus isolates, including virulent and attenuated strains, with no cross-reactivity to other viruses, such as Newcastle disease virus, Marek's disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, and egg-drop-syndrome virus. The results showed that its specificity was highly consistent with the agar-gel precipitation test (AGP). The diagnostic strip detected as low as 800 median egg lethal dose (ELD50) viruses in the IBDV BC6/85-infected sample, which was comparable with AC-ELISA (400 ELD50) and 32 times more sensitive than the AGP test (2.56 x 10(4) ELD50). In experimental infection, IBDV was detected in the bursa as early as 36 hr postinfection with the diagnostic strip before the clinical signs and gross lesions appeared. It takes only 1-2 min to do a strip test to detect chicken IBDV antigen after the specimen is grounded in a whirl pack with finger massage.
authors.affiliation: Institute of Biotechnology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Gai-Ping
authors.initials: GP
authors.lastname: Zhang
authors.forename: Qing-Mei
authors.initials: QM
authors.lastname: Li
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Yang
authors.forename: Jun-Qing
authors.initials: JQ
authors.lastname: Guo
authors.forename: Xue-Wu
authors.initials: XW
authors.lastname: Li
authors.forename: Rui-Guang
authors.initials: RG
authors.lastname: Deng
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Xiao
authors.forename: Guang-Xu
authors.initials: GX
authors.lastname: Xing
authors.forename: Ji-Fei
authors.initials: JF
authors.lastname: Yang
authors.forename: Dong
authors.initials: D
authors.lastname: Zhao
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Cai
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000302:isolation & purification*; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D002460:Cell Line; D002645:Chickens; D019937:Diagnostic Techniques and Procedures / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D007243:Infectious bursal disease virus / Q000276:immunology*; D051379:Mice; D011201:Poultry Diseases / Q000175:diagnosis*; D011817:Rabbits; D011934:Reagent Strips
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D011934:Reagent Strips