[Research on the protein C-activating property and influencial factors of protein C activator isolated from the venom of Agkistrodon halys].
OBJECTIVE
To investigate the protein C-activating property and experimental conditions that may affect the activities of protein C activator (PCA-SV).


METHODS
PCA-SV's functional characteristic, possible anticoagulant and amidase activities were measured by method of APTT and that of specific chromogenic substrate respectively in various experimental conditions.


RESULTS
PCA-SV can specifically act on protein C. The pH-optimum and temperature-optimum for the action of PCA-SV were 6.0 - 7.0 and 37 - 40 degrees C respectively. The anticoagulants (heparin, sodium citrate) had no effect on the activities.


CONCLUSIONS
PCA-SV is a new protein C-activating reagent that has high potency and specificity.
Hou
Liqiang
L
Research Institute of Snake Venom, Guangzhou Medical College, Guangzhou.
Zhao
Luning
L
Zhao
Zhongxi
Z
Huang
Shaoling
S
He
Quanhua
Q
Kong
Tianahn
T
Guan
Jinxia
J
D017836:Agkistrodon; D000581:Amidohydrolases / Q000097:blood; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D003435:Crotalid Venoms / Q000737:chemistry*; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D036341:Intercellular Signaling Peptides and Proteins; D008419:Materia Medica / Q000494:pharmacology*; D010314:Partial Thromboplastin Time; D010455:Peptides / Q000494:pharmacology; D011486:Protein C / Q000187:drug effects / Q000378:metabolism*; D013696:Temperature
D003435:Crotalid Venoms; D005343:Fibrinolytic Agents; D036341:Intercellular Signaling Peptides and Proteins; D008419:Materia Medica; D010455:Peptides; D011486:Protein C; C049647:snake venom protein C activator; D000581:Amidohydrolases