title: Berberine inhibits arylamine N-acetyltransferase activity and gene expression in Salmonella typhi.
abstract: The effects of berberine on growth, arylamine N-acetyltransferase (NAT) activity, and gene expression in Salmonella Typhi (Typhi) were described. The growth inhibition of Typhi was determined by measuring absorbance by optical density (OD at 650 nm). The NAT activity was determined by measuring the levels of 2-aminofluorene (AF) and N-acetyl-2-aminofluorene (AAF) by high-performance liquid chromatography. The results demonstrated that 24-h berberine treatment decreased bacteria growth and amounts of AAF in Typhi. Western blotting and flow cytometry were used for examining the levels of NAT after bacteria were cotreated with or without various concentrations of berberine, and results indicated that berberine decreased the levels of NAT in Typhi. Polymerase chain reaction was used for examining the gene expression of NAT (mRNA NAT), and results indicated that berberine affects mRNA NAT1 expression in Typhi.
authors.affiliation: Department of Microbiology, China Medical University, Taichung, Taiwan.
authors.forename: Lii-Tzu
authors.initials: LT
authors.lastname: Wu
authors.forename: Mei-Fen
authors.initials: MF
authors.lastname: Tsou
authors.forename: Chin-Chin
authors.initials: CC
authors.lastname: Ho
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Chuang
authors.forename: Hsiu-Maan
authors.initials: HM
authors.lastname: Kuo
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
mesh_terms: D001191:Arylamine N-Acetyltransferase / Q000378:metabolism*; D001599:Berberine / Q000494:pharmacology*; D028142:Berberis; D015536:Down-Regulation; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D012485:Salmonella typhi / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D001599:Berberine; D001191:Arylamine N-Acetyltransferase