title: Detecting correlation between sequence and expression divergences in a comparative analysis of human serpin genes.
abstract: Physiological functions and characteristic structures of the serpin gene superfamily have been studied extensively, yet the evolution of the serpin genes remains unclear. Gene duplication in this superfamily may shed light on this issue. Two models are used to predict the preservation of duplicated genes: the classical model and the duplication-degeneration-complementation (DDC) model. In this study, we analyzed the phylogenetic relationships of 33 human serpin genes and the expression data of some members of the serpin superfamily from a DNA microarray of human leukemia U937 cells with stably inducible expression of the leukemia-related AML1-ETO gene. We then determined the utility of the DDC model by mapping serpin superfamily expression data to the phylogenetic tree. The correlation between sequence and expression divergences as measured by the Pearson correlation coefficient indicated that human serpin genes evolved under the DDC model. Our study provides a new strategy for comparative analysis of gene sequences and microarray data.
authors.affiliation: School of Life Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Zuofeng
authors.initials: Z
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Mangen
authors.initials: M
authors.lastname: Song
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Peng
authors.initials: P
authors.lastname: Nan
authors.forename: Ying
authors.initials: Y
authors.lastname: Cao
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Chen
authors.forename: Yixue
authors.initials: Y
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhong
mesh_terms: D045744:Cell Line, Tumor; D019295:Computational Biology / Q000379:methods*; D019143:Evolution, Molecular; D020440:Gene Duplication; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D015843:Serpins / Q000737:chemistry* / Q000235:genetics*; D049490:Systems Biology; D020298:U937 Cells
chemical_list: D015843:Serpins