[Effect of IL-15 on the proliferation, differentiation and anti-apoptosis of CD34+ cells in patients with MDS].
To study the effect of interleukin-15 (IL-15) on the proliferation, differentiation and apoptosis of MDS CD34(+) cells, CD34(+) cells of high enrichment were separated by MACS system, and cultured in liquid media with different concentration of IL-15 in treated group and without IL-15 in the control group. Apoptosis of hematopoietic precursors was assayed by propidium iodine staining and cell by FCM, and the other MDS CD34(+) cells were stained by cytochemical staining after culture. The results showed that after culture with IL-15 the proliferation and differentiation of MDS CD34(+) cells were obviously promoted. It was found the every lineage of mature cells developed, the expressions of cell surface antigens CD71, CD33 and CD19 all increased in the MDS CD34(+) cell treated with IL-15. It is suggested that IL-15 stimulates the proliferation and differentiation of MDS CD34(+) cells, and partly shows anti-apoptosis effects which may be applicable to the therapy MDS.
Cheng
Ming-Zheng
MZ
Department of Pediatrics, Affiliated Hospital of Guangdong Medical College, Zhangjiang 524001, China.
Ye
Zhong-Lu
ZL
Cai
Kang-Rong
KR
Huang
Xiu-Lan
XL
Cheng
Ri-Ling
RL
Chen
Han-Rong
HR
D015703:Antigens, CD / Q000276:immunology; D018941:Antigens, CD19 / Q000276:immunology; D018952:Antigens, CD34 / Q000276:immunology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology; D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D019409:Interleukin-15 / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D009190:Myelodysplastic Syndromes / Q000097:blood / Q000276:immunology* / Q000473:pathology; D011990:Receptors, Transferrin / Q000276:immunology; D063268:Sialic Acid Binding Ig-like Lectin 3
D015703:Antigens, CD; D018941:Antigens, CD19; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; C068126:CD71 antigen; D019409:Interleukin-15; D011990:Receptors, Transferrin; D063268:Sialic Acid Binding Ig-like Lectin 3