title: Statistical methods for comparing comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry results: metabolomic analysis of mouse tissue extracts.
abstract: The potential for the utilization of GC x GC-time-of-flight (TOF) MS for high-resolution metabolomics studies is discussed, with the implementation of some statistical comparisons for biomarker detection. Metabolite profiles from NZO obese mice versus BL/6 control mice are compared and contrasted using a number of chromatogram comparison routines, including direct chromatogram comparisons, chromatogram subtraction and averaging routines, as well as a method for generating relative weighted peak surface difference chromatograms, and a more conventional Student's t-test statistical approach.
authors.affiliation: Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Shellie
authors.forename: Werner
authors.initials: W
authors.lastname: Welthagen
authors.forename: Jitka
authors.initials: J
authors.lastname: Zrostliková
authors.forename: Joachim
authors.initials: J
authors.lastname: Spranger
authors.forename: Michael
authors.initials: M
authors.lastname: Ristow
authors.forename: Oliver
authors.initials: O
authors.lastname: Fiehn
authors.forename: Ralf
authors.initials: R
authors.lastname: Zimmermann
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D051379:Mice