title: [Recombinant MMP-14 catalytic domain inducing the apoptosis of human osteoblastic SaOS-2 cells].
abstract: OBJECTIVE
  To investigate the direct effects of recombinant matrix metalloproteinase-14 (MMP-14) catalytic domain (RMC) on cultured human osteoblastic SaOS-2 cells.
  
  
  METHODS
  Western Blotting was used to analyze the expression of MMP-14 in cultured media. The effect of RMC on activation of proMMP-2 was measured by gelatin zymograms analysis, and the influence of RMC on cells adhesion was analyzed as well. The apoptosis was detected by acidine orange/ethidium bromide staining and ELISA.
  
  
  RESULTS
  RMC activated proMMP-2 secreted into media by SaOS-2 cells, and this process was blocked by EDTA treatment. RMC inhibited the adhesion of SaOS-2 cells to immobilized Type I collagen in a dose-dependent manner, which was also abolished by EDTA. RMC induced SaOS-2 cells apoptosis in a dose-dependent manner, and apoptosis-inducing activity of MMP-14 catalytic domain was blocked by EDTA.
  
  
  CONCLUSIONS
  Since adhesion of cells to extracellular matrix serves as a survival mechanism in osteoblasts, the catalytic activity of recombinant MMP-14 catalytic domain on matrix proteins contributes to its apoptosis-inducing activity.
authors.affiliation: Institute of Endocrinology and Metabolism, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Guo
authors.forename: Xiang-Hang
authors.initials: XH
authors.lastname: Luo
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.forename: Hou-De
authors.initials: HD
authors.lastname: Zhou
authors.forename: Er-Yuan
authors.initials: EY
authors.lastname: Liao
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D020134:Catalytic Domain; D002478:Cells, Cultured; D006801:Humans; D020782:Matrix Metalloproteinases / Q000494:pharmacology*; D053504:Matrix Metalloproteinases, Membrane-Associated; D010006:Osteoblasts / Q000166:cytology*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D011994:Recombinant Proteins; D020782:Matrix Metalloproteinases; D053504:Matrix Metalloproteinases, Membrane-Associated