title: Rapid PrP(Sc) detection in lymphoid tissue and application to scrapie surveillance of fallen stock in Japan: variable PrP(Sc) accumulation in palatal tonsil in natural scrapie.
abstract: Rapid western blot (WB) procedure for an abnormal isoform of prion protein (PrP(Sc) ) detection in lymphoid tissues was established and has been applied to the surveillance of fallen stock. In this program, brain and palatal tonsil were examined by WB and three cases of sheep scrapie were detected. While one clinically scrapie-infected sheep harbored PrP(Sc) in the brain and palatal tonsil, the two sheep in the pre-clinical stage harbored PrP(Sc) in the brain, but not in the palatal tonsil. This study shows that PrP(Sc) accumulation in palatal tonsil is variable in natural scrapie, even among genetically susceptible sheep.
authors.affiliation: Prion Disease Research Center, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: Kimi
authors.initials: K
authors.lastname: Shimada
authors.forename: Hiroko K
authors.initials: HK
authors.lastname: Hayashi
authors.forename: Yuka
authors.initials: Y
authors.lastname: Ookubo
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Iwamaru
authors.forename: Morikazu
authors.initials: M
authors.lastname: Imamura
authors.forename: Masuhiro
authors.initials: M
authors.lastname: Takata
authors.forename: Mary Jo
authors.initials: MJ
authors.lastname: Schmerr
authors.forename: Morikazu
authors.initials: M
authors.lastname: Shinagawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D020022:Genetic Predisposition to Disease; D007564:Japan; D008221:Lymphoid Tissue / Q000737:chemistry*; D014066:Palatine Tonsil / Q000737:chemistry; D018620:PrPSc Proteins / Q000032:analysis* / Q000235:genetics / Q000472:pathogenicity; D012608:Scrapie / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism*; D012756:Sheep
chemical_list: D018620:PrPSc Proteins