Structural proteomics of macromolecular assemblies using oxidative footprinting and mass spectrometry.
Understanding the composition, structure and dynamics of macromolecules and their assemblies is at the forefront of biological science today. Hydroxyl-radical-mediated protein footprinting using mass spectrometry can define macromolecular structure, macromolecular assembly and conformational changes of macromolecules in solution based on measurements of reactivity of amino acid side-chain groups with covalent-modification reagents. Subsequent to oxidation by reactive oxygen species, proteins are digested by specific proteases to generate peptides for analysis by mass spectrometry. Accurate measurements of side-chain reactivity are achieved using quantitative liquid-chromatography-coupled mass spectrometry, whereas the side-chain sites within the macromolecular probes are identified using tandem mass spectrometry. In addition, the use of footprinting data in conjunction with computational modeling approaches is a powerful new method for testing and refining structural models of macromolecules and their complexes.
Guan
Jing-Qu
JQ
Case Center for Proteomics and Mass Spectrometry, 930 BRB, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Chance
Mark R
MR
D002853:Chromatography, Liquid; D019295:Computational Biology; D017665:Hydroxyl Radical; D046911:Macromolecular Substances; D013058:Mass Spectrometry; D008961:Models, Structural; D010084:Oxidation-Reduction; D011487:Protein Conformation; D023302:Protein Footprinting; D011506:Proteins / Q000378:metabolism; D040901:Proteomics; D017382:Reactive Oxygen Species; D013329:Structure-Activity Relationship
D046911:Macromolecular Substances; D011506:Proteins; D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical