title: Effect of NAD on recovery of adenine nucleotide pool, phosphorylation potential, and stimulation of apoptosis during late period of reperfusion damage to myocardium.
abstract: The system of energy supply in the myocardium of the left and right ventricles did not recover after short-term circulatory disturbances. ATP synthesis decreased in parallel with activation of poly-(ADP-ribose)-polymerase in the ischemic region of the right ventricle, extra-ischemic region, and in the left ventricle by 5.85, 5.4, and 2.2 times, respectively. Intravenous injection of NAD immediately after blood flow resumption in the subacute period of ischemia-reperfusion damage virtually completely normalized the pool of adenine nucleotides, energy change of the adenine nucleotide system, and phosphorylation potential. Exogenous NAD inhibited activity of poly-(ADP-ribose)-polymerase in the ischemic region of the right ventricle, extra-ischemic region, and in the ischemic region of the left ventricle by 2.4, 2.9, and 1.52 times, respectively. We hypothesize that NAD acts as a regulator of signal mechanism of apoptosis induction during ischemia-reperfusion damages to the myocardium.
authors.affiliation: N. V. Karsanov Republican Research Center for Medical Biophysics and New Biomedical Technologies, Tbilisi.
authors.forename: G V
authors.initials: GV
authors.lastname: Sukoyan
authors.forename: N A
authors.initials: NA
authors.lastname: Andriadze
authors.forename: E I
authors.initials: EI
authors.lastname: Guchua
authors.forename: N V
authors.initials: NV
authors.lastname: Karsanov
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D004285:Dogs; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009243:NAD / Q000494:pharmacology* / Q000502:physiology; D010725:Phosphocreatine / Q000378:metabolism; D010766:Phosphorylation; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism
chemical_list: D000227:Adenine Nucleotides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D010725:Phosphocreatine; D009243:NAD; D000255:Adenosine Triphosphate; D011065:Poly(ADP-ribose) Polymerases