title: Genome-wide map of nucleosome acetylation and methylation in yeast.
abstract: Eukaryotic genomes are packaged into nucleosomes whose position and chemical modification state can profoundly influence regulation of gene expression. We profiled nucleosome modifications across the yeast genome using chromatin immunoprecipitation coupled with DNA microarrays to produce high-resolution genome-wide maps of histone acetylation and methylation. These maps take into account changes in nucleosome occupancy at actively transcribed genes and, in doing so, revise previous assessments of the modifications associated with gene expression. Both acetylation and methylation of histones are associated with transcriptional activity, but the former occurs predominantly at the beginning of genes, whereas the latter can occur throughout transcribed regions. Most notably, specific methylation events are associated with the beginning, middle, and end of actively transcribed genes. These maps provide the foundation for further understanding the roles of chromatin in gene expression and genome maintenance.
authors.affiliation: Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA.
authors.forename: Dmitry K
authors.initials: DK
authors.lastname: Pokholok
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Harbison
authors.forename: Stuart
authors.initials: S
authors.lastname: Levine
authors.forename: Megan
authors.initials: M
authors.lastname: Cole
authors.forename: Nancy M
authors.initials: NM
authors.lastname: Hannett
authors.forename: Tong Ihn
authors.initials: TI
authors.lastname: Lee
authors.forename: George W
authors.initials: GW
authors.lastname: Bell
authors.forename: Kimberly
authors.initials: K
authors.lastname: Walker
authors.forename: P Alex
authors.initials: PA
authors.lastname: Rolfe
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Herbolsheimer
authors.forename: Julia
authors.initials: J
authors.lastname: Zeitlinger
authors.forename: Fran
authors.initials: F
authors.lastname: Lewitter
authors.forename: David K
authors.initials: DK
authors.lastname: Gifford
authors.forename: Richard A
authors.initials: RA
authors.lastname: Young
mesh_terms: D000107:Acetylation; D002874:Chromosome Mapping / Q000379:methods; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics; D016681:Genome, Fungal; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D008745:Methylation; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D006657:Histones; D009707:Nucleosomes