[Targeted killing effect of mucin-1 single chain antibody targeting, lentivirus-mediated suicide gene therapy and ganciclovir in mucin-1+ ovarian epithelial carcinoma cells].
OBJECTIVE
To investigate the targeted killing effect of mucin-1 single chain antibody targeting, lentivirus-mediated suicide gene therapy and ganciclovir(GCV) in mucin-1+ ovarian epithelial carcinoma cells.


METHODS
Mucin-1 single chain antibody targeting lentivirus produced by packaging cell line 293T transduced herpes simplex virus thymidine kinase (HSV-tk) gene into the ovarian epithelial cancer cell line SKOV3 (MUC1+). The infection effect was observed through fluorescence microscopy. Polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) were resorted to demonstrate the successful transduction and transcription of the HSV-tk gene. After administration of GCV, changes of those cells were observed through optical microscopy. The cytotoxicity efficacy of HSV-tk/GCV system was evaluated by methyl thiazolyl tetrazolium method.


RESULTS
It was observed through fluorescence microscopy that anti-MUC1 directed lentivirus can specificly infect MUC1+ ovarian cancer cells. A fragment of 600 bp was generated through PCR and RT-PCR which indicated successful transduction and transcription of the HSV-tk gene in SKOV3 cells. Changes of cells followed by administration of GCV were observed with optical microscopy. Significant cytotoxicity efficacy of GCV to SKOV3 was observed.


CONCLUSIONS
The HSV-tk gene can be targetedly transducted into MUC1+ ovarian cancer cell line under the mediation of anti-MUC1 directed lentivirus, and such HSV-tk/GCV system has targetingly killing effect on MUC1+ cancer cells.
Song
L
L
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China.
Kong
B
B
Liu
C
C
D000914:Antibodies, Viral; D000998:Antiviral Agents / Q000627:therapeutic use; D002277:Carcinoma / Q000628:therapy*; D045744:Cell Line, Tumor; D005260:Female; D015774:Ganciclovir / Q000627:therapeutic use; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008856:Microscopy, Fluorescence; D018396:Mucin-1 / Q000276:immunology*; D010051:Ovarian Neoplasms / Q000628:therapy*; D016133:Polymerase Chain Reaction; D014161:Transduction, Genetic
D000914:Antibodies, Viral; D000998:Antiviral Agents; D018396:Mucin-1; D015774:Ganciclovir