Albumin and urea production by hepatocytes cultured on extracellular matrix proteins-conjugated poly(vinyl alcohol) membranes.
Production of albumin and urea by mouse hepatocytes on poly(vinylalcohol-co-ethylamine) (PVA-EA) membranes containing immobilized extracellular matrix (ECM) proteins was investigated for 7 days. The amount of ECM proteins (collagen, vitronectin and laminin) immobilized on PVA-EA and PVA-ECM membranes was determined to range from 1.09 microg/cm2 to 1.60 microg/cm2. Hepatocytes cultured on PVA-ECM membranes in serum-free media showed higher albumin production than those cultured on PVA-EA membranes after a 7-day incubation under the conditions in this study. Urea production by hepatocytes on PVA-ECM membranes was also determined to be higher than that on PVA-EA membranes up until day 5 of incubation in serum-free media, whereas no difference of urea production by hepatocytes on different PVA-ECM membranes and PVA-EA membranes was observed at 7 days of incubation. The effect of ECM proteins in PVA-ECM membranes on hepatocyte function (such as albumin and urea production) was observed in hepatocytes cultured in serum-free media up to day 5 of incubation. The ECM proteins immobilized on the PVA-ECM membranes contributed not only to the long-term stable production of albumin and urea by hepatocytes, but also the improved surviVal (viability) of hepatocytes on PVA-ECM membranes.
Higuchi
Akon
A
Department of Applied Chemistry, Seikei University, 3-3-1 Kichijoji Kitamachi, Musashino, Tokyo 180-8633, Japan. higuchi@ch.seikei.ac.jp
Kurihara
Marie
M
Kobayashi
Kenichi
K
Cho
Chong Su
CS
Akaike
Toshihiro
T
Hara
Mariko
M
D000418:Albumins / Q000096:biosynthesis*; D000818:Animals; D018929:Cell Culture Techniques; D048708:Cell Growth Processes / Q000502:physiology; D005021:Ethylamines; D016326:Extracellular Matrix Proteins; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D008567:Membranes, Artificial; D051379:Mice; D008813:Mice, Inbred ICR; D011142:Polyvinyl Alcohol; D013997:Time Factors; D014508:Urea / Q000378:metabolism*
D000418:Albumins; D005021:Ethylamines; D016326:Extracellular Matrix Proteins; D008567:Membranes, Artificial; C478709:poly(vinyl alcohol-co-ethylamine); D014508:Urea; D011142:Polyvinyl Alcohol