[Effect of EGCG on H2O2-induced MnSOD gene expression in cultured spiral ganglion cells].
OBJECTIVE
To investigate the influence of EGCG on H2O2-induced gene expression of manganese superoxide dismutase (MnSOD or SOD2) in cultured spiral ganglion cells (SGCs) in vitro.


METHODS
SGCs were cultured in vitro, and H2O2 and/or EGCG in different concentrations were used. Semi-quantitative RT-PCR was applied to observe MnSOD gene expression in SGCs after H2O2 and EGCG treatment.


RESULTS
The expression of MnSOD gene was up-regulated with the increase of the concentration of H2O2 in cultured SGCs, and MnSOD gene expression was significantly up-regulated at a dose of H2O2 > or =100 micromol/L. However, this up-regulation was suppressed after simultaneously treated with 100 microg/ml EGCG.


CONCLUSIONS
EGCG suppresses H2O2-induced up-regulation of MnSOD gene expression in cultured SGCs by getting rid of oxygen free radicals, reinforcing the activity of antioxidant enzymes such as MnSOD, and protects cultured SGCs from H2O2-induced oxidizing damage.
Liu
Guo-hui
GH
Department of Otorhinolaryngology, Third Xiangya Hospital, Central South University, Changsha 410013, China. guohliu@hotmail.com
Xie
Ding-hua
DH
Zhu
Gang-hua
GH
Wu
Wei-jing
WJ
Ge
Sheng-lei
SL
D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D003051:Cochlea / Q000166:cytology / Q000294:innervation; D005260:Female; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D051379:Mice; D013136:Spiral Ganglion / Q000166:cytology / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics
D002392:Catechin; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate; D013482:Superoxide Dismutase