Syngeneic mouse mammary carcinoma cell lines: two closely related cell lines with divergent metastatic behavior.
Two cell lines, Met-1(fvb2) and DB-7(fvb2), with different metastatic potential, were derived from mammary carcinomas in FVB/N-Tg(MMTV-PyVmT) and FVB/N-Tg(MMTV-PyVmT ( Y315F/Y322F )) mice, transplanted into syngeneic FVB/N hosts and characterized. The lines maintain a stable morphological and biological phenotype after multiple rounds of in vitro culture and in vivo transplantation. The Met-1(fvb2) line derived from a FVB/N-Tg(MMTV-PyVmT) tumor exhibits invasive growth and 100% metastases when transplanted into the females FVB/N mammary fat pad. The DB-7(fvb2) line derived from the FVB/N-Tg(MMTV-PyVmT ( Y315F/Y322F )) with a "double base" modification at Y315F/Y322F exhibits more rapid growth when transplanted into the mammary fat pad, but a lower rate of metastasis (17%). The Met1(fvb2) cells show high activation of AKT, while DB-7(fvb2) cells show very low levels of AKT activation. The DNA content and gene expression levels of both cell lines are stable over multiple generations. Therefore, these two cell lines provide a stable, reproducible resource for the study of metastasis modulators, AKT molecular pathway interactions, and gene target and marker discovery.
Borowsky
Alexander D
AD
Department of Medical Pathology and Laboratory Medicine, UC Davis School of Medicine, Sacramento, CA 95817, USA. adborowsky@ucdavis.edu
Namba
Ruria
R
Young
Lawrence J T
LJ
Hunter
Kent W
KW
Hodgson
J Graeme
JG
Tepper
Clifford G
CG
McGoldrick
Erik T
ET
Muller
William J
WJ
Cardiff
Robert D
RD
Gregg
Jeffrey P
JP
D000818:Animals; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D009362:Neoplasm Metastasis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt; D011960:Receptors, Estrogen / Q000032:analysis / Q000378:metabolism
D011518:Proto-Oncogene Proteins; D011960:Receptors, Estrogen; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt