High-performance liquid chromatographic assay for the active saponins from Panax notoginseng in rat tissues.
A reversed-phase liquid chromatographic method was used to determine the ginsenosides Rg1, Rb1 and Rd of Panax notoginseng in rat tissues (kidney, liver, heart, spleen and lung) after the administration of total saponins of P. notoginseng. The tissue samples were treated with solid-phase extraction prior to HPLC. The calibration curves for the three saponins were linear in the given concentration ranges. The intra-day and inter-day assay coefficients in tissues were between 76 and 120% respectively. The recoveries of all the tissues were higher than 70%. This method was applied to evaluate the distribution of the three major saponins of P. notoginseng in rat tissues.
Li
Lie
L
School of Pharmaceutical Sciences and Modern Research Center for Traditional Chinese Medicine, Peking University, Beijing, 100083, People's Republic of China.
Sheng
Yu-Xin
YX
Zhang
Jin-Lan
JL
Wang
Shan-Shan
SS
Guo
De-An
DA
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D036145:Ginsenosides / Q000032:analysis* / Q000493:pharmacokinetics*; D008297:Male; D005894:Panax / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D014018:Tissue Distribution
D036145:Ginsenosides; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1; C049863:ginsenoside Rd