title: Interaction of enteropathogenic and Shiga toxin-producing Escherichia coli and porcine intestinal mucosa: role of intimin and Tir in adherence.
abstract: The ileal in vitro organ culture (IVOC) model using tissues originating from colostrum-deprived newborn piglets has proven to be an effective way to study the attaching and effacing (A/E) phenotype of porcine enteropathogenic Escherichia coli (EPEC) ex vivo. The aim of this study was to investigate the role of intimin subtype and Tir in the adherence of EPEC and Shiga-toxin-producing E. coli (STEC), isolated from different animal species, to porcine intestinal IVOC. Moreover, the role of intimin in Tir-independent adherence of the human EPEC strain E2348/69 was investigated using intimin and Tir-deficient derivatives. Our results demonstrated that A/E E. coli strains (AEEC) from various animal species and humans induce the A/E phenotype in porcine ileal IVOC and that intimin subtype influences intestinal adherence and tropism of AEEC strains. We also showed that a tir mutant of EPEC strain E2348/69 demonstrates close adherence to the epithelial cells of porcine ileal IVOC segments, with microvillous effacement but with no evidence of actin polymerization or pedestal formation, and that intimin seems to be involved in this phenotype. Overall, this study provides further evidence for the existence of one or more host-cell-encoded intimin receptor(s) in the pig gut.
authors.affiliation: Groupe de Recherche sur les Maladies Infectieuses du Porc, Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Canada.
authors.forename: Francis
authors.initials: F
authors.lastname: Girard
authors.forename: Isabelle
authors.initials: I
authors.lastname: Batisson
authors.forename: Gad M
authors.initials: GM
authors.lastname: Frankel
authors.forename: Josée
authors.initials: J
authors.lastname: Harel
authors.forename: John M
authors.initials: JM
authors.lastname: Fairbrother
mesh_terms: D018829:Adhesins, Bacterial / Q000502:physiology*; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D006790:Host-Parasite Interactions / Q000502:physiology; D007082:Ileum / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D023421:Models, Animal; D009154:Mutation; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D022621:Shiga Toxin / Q000378:metabolism*; D013552:Swine
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D011956:Receptors, Cell Surface; C109287:Tir protein, E coli; C073819:eaeA protein, E coli; D022621:Shiga Toxin