A study of mutations in the c-kit gene of 32 dogs with mastocytoma.
Mutations in the intracellular juxtamembrane domain of the c-kit gene in 32 dogs with different grades of histologically confirmed mastocytoma were studied. Transcript RNAs extracted from neoplastic tissue surgically collected from dogs of different breeds and from a negative control were reverse transcribed into complementary DNA and amplified by polymerase chain reaction. The region corresponding to the c-kit juxtamembrane domain was sequenced and compared with GenBank sequences. Two different types of mutations were identified within exon 11: a previously underscribed single-nucleotide substitution and a 6-bp deletion. The c-kit juxtamembrane domain sequences of all dogs were grouped in 3 clusters. No mutations were detected in tissues constitutively expressing c-kit (cerebellum and spleen), obtained from dogs not affected by mastocytoma (controls). All the substitutions were found in dogs bearing grade I or II mast cell tumors; the deletion was detected in 1 dog with grade II mastocytoma.
Riva
Federica
F
Dipartimento di Patologia Animale, Igiene e Sanita' Pubblica Veterinaria, Universita' degli Studi di Milano, Italy.
Brizzola
Stefano
S
Stefanello
Damiano
D
Crema
Simone
S
Turin
Lauretta
L
D000818:Animals; D001483:Base Sequence; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005260:Female; D008297:Male; D034801:Mastocytoma / Q000235:genetics / Q000662:veterinary*; D008969:Molecular Sequence Data; D009154:Mutation; D009857:Oncogenes; D016133:Polymerase Chain Reaction / Q000662:veterinary; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D012334:RNA, Neoplasm / Q000737:chemistry
D012334:RNA, Neoplasm; D019009:Proto-Oncogene Proteins c-kit