title: Leishmania (Viannia) braziliensis growth in vitro culture relies more on folic acid availability than Leihsmania (Leishmania) amazonensis.
abstract: We compared the in vitro growth of promastigotes from two Leishmania species in TC-100 and Schneider media. Leishmania (Leishmania) amazonensis replication rates were similar in both tissue culture media and reached maximum rates by 48 h. In contrast Leishmania (Viannia) braziliensis growth was significantly greater in TC-100 but maximum rates were achieved by 96 h. Folic acid appears to be the limiting factor and supplementation of Schneider media with this nutrient improved L. (V.) braziliensis replication rates and decreased the time of maximum replication to 48 h.
authors.affiliation: Laboratorio de Biofísica, Centro Internacional de Física, Bogotá, Colombia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Niño
authors.forename: Marcela
authors.initials: M
authors.lastname: Camacho
mesh_terms: D000818:Animals; D003470:Culture Media; D005492:Folic Acid / Q000378:metabolism*; D007891:Leishmania / Q000145:classification / Q000254:growth & development* / Q000378:metabolism; D007892:Leishmania braziliensis / Q000145:classification / Q000254:growth & development / Q000378:metabolism; D013045:Species Specificity
chemical_list: D003470:Culture Media; D005492:Folic Acid