title: [Studies on subset, location and secretion of natural keratin-reactive B cells].
abstract: OBJECTIVE
  To clarify the subsets and location of B cells responsible for the production of natural anti-keratin autoantibody (AK auto Ab), and analyze the function of each subset.
  
  
  METHODS
  Cells isolated from spleen and peritoneal cavity of C57BL/6 mice were cultured in vitro. FACS was employed to detect keratin reactive B cell subsets. The anti-keratin IgM level in supernatant was analyzed by ELISA, while ELISPOT was utilized to detect the B cells which can secrete AK auto Ab.
  
  
  RESULTS
  Keratin reactive B cells were mainly marginal zone B cells in spleen, while they were mainly B-1a cells in peritoneal cavity. There were more B cells producing AK auto Ab in peritoneal cavity than in spleen. The anti-keratin IgM level in the supernatant of cultured peritoneal cells was much higher than that of splenocytes.
  
  
  CONCLUSIONS
  There are three subsets of keratin-reactive B cells: B-1 B cells, follicular B cells and marginal zone B cells. B-1 B cells are active in secreting AK auto Ab.
authors.affiliation: Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. xyshadowxy@yahoo.com.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Xing
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Meng
authors.initials: M
authors.lastname: Fu
authors.forename: Hua
authors.initials: H
authors.lastname: Han
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Liu
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007633:Keratins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D007633:Keratins