Liquid chromatography-diode array detection-electrospray ionisation mass spectrometry/nuclear magnetic resonance analyses of the anti-hyperglycemic flavonoid extract of Genista tenera. Structure elucidation of a flavonoid-C-glycoside.
The anti-hyperglycemic flavonoid extract obtained from Genista tenera was first studied by liquid chromatography (LC)-diode array detection (DAD) which showed the presence of two major compounds. One of them was identified as genistein-7-O-glucoside. Luteolin-7-O-glucoside was detected as a minor constituent, while luteolin-7,3'-di-O-glucoside and rutin were found in trace amounts. LC-DAD-ESI-MS and NMR were used to confirm the structure of these compounds and allowed the elucidation of the structure of the unknown major compound, which is the flavonoid 5,7,4'-trihydroxyisoflavone-8-C-glucoside.
Rauter
Amélia P
AP
Departamento de Química e Bioquímica/Centro de Química e Bioquímica, Faculdade de Ciências da Universidade de Lisboa, Ed C8, 5 Piso, 1749-016 Lisboa, Portugal. aprauter@fc.ul.pt
Martins
Alice
A
Borges
Carlos
C
Ferreira
Joana
J
Jorge
Justino
J
Bronze
Maria-Rosário
MR
Coelho
Ana V
AV
Choi
Young H
YH
Verpoorte
Robert
R
D002236:Carbohydrate Conformation; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D039761:Genista / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D005419:Flavonoids; D006027:Glycosides; D010936:Plant Extracts