title: Complex II inactivation is lethal in the nematode Caenorhabditis elegans.
abstract: RNA-mediated interference (RNAi) was employed to systematically inactivate the four subunits of complex II in the mitochondrial electron transport chain. Embryonic lethality was the predominant result of inactivating three subunits (ceSDHB, ceSDHC, and ceSDHD) when using the soaking method to inactivate RNA. The feeding method was employed to deliver dsRNA from the fourth subunit (ceSDHA) to wild-type, mev-1 (mutated in ceSDHC of complex II), and gas-1 animals (mutated in a complex I gene). Survival was reduced only in the mev-1 genetic background, and in an oxygen-dependent fashion. Collectively, these data provide further evidence that compromised complex II integrity can result in sensitivity to oxidative stress.
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Harumi
authors.initials: H
authors.lastname: Ichimiya
authors.forename: R Giselle
authors.initials: RG
authors.lastname: Huet
authors.forename: Phil
authors.initials: P
authors.lastname: Hartman
authors.forename: Hisako
authors.initials: H
authors.lastname: Amino
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kita
authors.forename: Naoaki
authors.initials: N
authors.lastname: Ishii