title: Dynamic assays of inhibin B, anti-Mullerian hormone and estradiol following FSH stimulation and ovarian ultrasonography as predictors of IVF outcome.
abstract: BACKGROUND
  We evaluated basal and dynamic hormonal markers [(FSH, inhibin B, estradiol and anti-Mullerian hormone (AMH)] during the follicular phase and luteal phase of the menstrual cycle and ultrasonic ovarian morphology as predictors of IVF outcome.
  
  
  METHODS
  Fifty-six women, aged <38 years, with normal day 3 FSH levels were included prospectively. Serum estradiol, inhibin B and AMH were measured before and 24 h after administration of 300 IU of recombinant FSH on cycle day 3-4 and during the luteal phase. Ovarian volume and antral follicle count (AFC) were evaluated on cycle day 3-4. The predictive value of oocyte number and pregnancy were assessed using uni- and multivariate analysis.
  
  
  RESULTS
  Poor responders (<6 oocytes) had significantly lower luteal AMH levels, while high responders (>20 oocytes) had significantly higher AFC, AMH and luteal stimulated inhibin B and estradiol than normal responders. Multivariate regression analyses showed that the best models for predicting oocyte number included AFC, follicular phase AMH and stimulated inhibin B. Only AMH showed a significant difference between pregnant and non-pregnant women at both cycle phases.
  
  
  CONCLUSIONS
  In young women (<38 years), AFC or basal AMH and stimulated inhibin B predict ovarian response for IVF. The only predictor for pregnancy is follicular or luteal phase AMH.
authors.affiliation: IVF Unit, Department of Obstetrics and Gynecology, Shaare-Zedek Medical Center, Ben Gurion University of the Negev, Jerusalem 91031, Israel. gevat@szmc.org.il
authors.forename: Talia
authors.initials: T
authors.lastname: Eldar-Geva
authors.forename: Avraham
authors.initials: A
authors.lastname: Ben-Chetrit
authors.forename: Irving M
authors.initials: IM
authors.lastname: Spitz
authors.forename: Ron
authors.initials: R
authors.lastname: Rabinowitz
authors.forename: Einat
authors.initials: E
authors.lastname: Markowitz
authors.forename: Tzvia
authors.initials: T
authors.lastname: Mimoni
authors.forename: Michael
authors.initials: M
authors.lastname: Gal
authors.forename: Edit
authors.initials: E
authors.lastname: Zylber-Haran
authors.forename: Ehud J
authors.initials: EJ
authors.lastname: Margalioth
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone; D002452:Cell Count; D004958:Estradiol / Q000097:blood*; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000627:therapeutic use*; D005498:Follicular Phase / Q000502:physiology; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007265:Inhibins / Q000097:blood*; D008183:Luteal Phase / Q000502:physiology; D009865:Oocytes / Q000166:cytology; D006080:Ovarian Follicle / Q000166:cytology; D010053:Ovary / Q000000981:diagnostic imaging*; D011247:Pregnancy; D011379:Prognosis; D013735:Testicular Hormones / Q000097:blood*; D016896:Treatment Outcome; D014463:Ultrasonography
chemical_list: D006023:Glycoproteins; D013735:Testicular Hormones; C422364:inhibin B; D004958:Estradiol; D007265:Inhibins; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone