Plant and animal homeodomains use convergent mechanisms for intercellular transfer.
Homeoproteins are defined by the structure of their DNA-binding domain, the homeodomain. Intercellular transfer of homeoprotein was observed ex vivo between animal cells and in vivo in higher plants. In the latter case, transfer is through intercytoplasmic channels that connect plant cells, but these do not exist in animals. Here, we show that the homeodomain of KNOTTED1, a maize homeoprotein, is transferred between animal cells and that a mutation in the homeodomain blocking the intercellular transfer of KNOTTED1 in plants also inhibits the transfer of the KNOTTED1 homeodomain in animal cells. This mutation decreases nuclear addressing, and its effect on nuclear import and intercellular transfer is reverted by the addition of an ectopic nuclear localization signal. We propose that, despite evolutionary distance and the differences in multicellular organization, similar mechanisms are at work for intercellular transfer of homeoprotein in plants and animals. Furthermore, our results suggest that, at least in animals, homeodomain secretion requires passage through the nucleus.
Tassetto
Michel
M
Homeoprotein Cell Biology, CNRS UMR 8542, Ecole Normale Supérieure, 46 rue d'Ulm, 75005 Paris, France.
Maizel
Alexis
A
Osorio
Joana
J
Joliot
Alain
A
D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D015153:Blotting, Western; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000502:physiology; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D003313:Zea mays / Q000235:genetics*
D018398:Homeodomain Proteins; C095848:Kn1 protein, plant; D010940:Plant Proteins