title: A conductive ormosil encapsulated with ferrocene conjugate and multiwall carbon nanotubes for biosensing application.
abstract: Highly non-toxic and conductive ormosil composite film was prepared using (3-aminopropyl)triethoxysilane and 2-(3,4-epoxycyclohexyl)-ethyltrimethoxysilane by doping with ferrocenemonocarboxylic acid-bovine serum albumin (FMC-BSA) conjugate and multiwall carbon nanotubes (MWNTs). With glucose oxidase (GOD) as a model enzyme this film could be used to design an amperometric biosensor for glucose determination. The entrapped FMC-BSA conjugate performed excellent redox electrochemistry and the immobilized GOD was highly stable. Under optimal conditions this biosensor was able to detect glucose with a detection limit of 20 microm (S/N=3) in the linear range of 0.05-20.0 mm in flow system, which was wider than the batch amperometric mode, with an analysis time of 25 s for each sample. The value of K(M)(app) was 6.6 mm. The proximity of these three components FMC-BSA, MWNTs and GOD enhanced the electron transfer between the film and electrode. This film could be used efficiently for the entrapment of other redox bioactive compounds and biosensing/bioelectrochemical applications.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science, Education Ministry of China, Department of Chemistry, Nanjing University, Nanjing 210093, China.
authors.forename: Vivek Babu
authors.initials: VB
authors.lastname: Kandimalla
authors.forename: Vijay Shyam
authors.initials: VS
authors.lastname: Tripathi
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D005296:Ferrous Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D000075163:Metallocenes; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size
chemical_list: D020099:Coated Materials, Biocompatible; D005296:Ferrous Compounds; D000075163:Metallocenes; D037742:Nanotubes, Carbon; D005949:Glucose Oxidase; D005947:Glucose; C004998:ferrocene