A salt-bridge motif involved in ligand binding and large-scale domain motions of the maltose-binding protein.
The uptake of nutrients is essential for the survival of bacterial cells. Many specialized systems have evolved, such as the maltose-dependent ABC transport system that transfers oligosaccharides through the cytoplasmic membrane. The maltose/maltodextrin-binding protein (MBP) serves as an initial high-affinity binding component in the periplasm that delivers the bound sugar into the cognate ABC transporter MalFGK(2). We have investigated the domain motions induced by the binding of the ligand maltotriose into the binding cleft using molecular dynamics simulations. We find that MBP is predominantly in the open state without ligand and in the closed state with ligand bound. Oligosaccharide binding induces a closure motion (30.0 degrees rotation), whereas ligand removal leads to domain opening (32.6 degrees rotation) around a well-defined hinge affecting key areas relevant for chemotaxis and transport. Our simulations suggest that a "hook-and-eye" motif is involved in the binding. A salt bridge between Glu-111 and Lys-15 forms that effectively locks the protein-ligand complex in a semiclosed conformation inhibiting any further opening and promoting complete closure. This previously unrecognized feature seems to secure the ligand in the binding site and keeps MBP in the closed conformation and suggests a role in the initial steps of substrate transport.
Stockner
Thomas
T
Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta T2N 1N4, Canada.
Vogel
Hans J
HJ
Tieleman
D Peter
DP
D020816:Amino Acid Motifs; D001224:Aspartic Acid / Q000737:chemistry; D001665:Binding Sites; D001692:Biological Transport; D002244:Carbon / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D002633:Chemotaxis; D018360:Crystallography, X-Ray; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005998:Glycine / Q000737:chemistry; D008024:Ligands; D008239:Lysine / Q000737:chemistry; D008320:Maltose / Q000737:chemistry; D058619:Maltose-Binding Proteins; D008958:Models, Molecular; D009844:Oligosaccharides / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012492:Salts / Q000737:chemistry*; D013997:Time Factors
D002352:Carrier Proteins; D008024:Ligands; D058619:Maltose-Binding Proteins; D009844:Oligosaccharides; D012492:Salts; D001224:Aspartic Acid; D008320:Maltose; D002244:Carbon; D008239:Lysine; D005998:Glycine