Production of rosmarinic acid and a new rosmarinic acid 3'-O-beta-D-glucoside in suspension cultures of the hornwort Anthoceros agrestis Paton.
Cell suspension cultures of the hornwort Anthoceros agrestis Paton (Anthocerotaceae) were cultivated and characterized in CB-media containing 2 and 4% sucrose. The suspension cells accumulated rosmarinic acid up to 5.1% of the cell dry weight as well as caffeoyl-4'-hydroxyphenyllactate. Moreover, a more hydrophilic compound was detected which was isolated and identified as rosmarinic acid 3'-O-beta-D-glucoside, a new rosmarinic acid derivative. This new rosmarinic acid derivative was found up to 1.0% of the cell dry weight in suspension cells of A. agrestis.
Vogelsang
Katharina
K
Institut für Pharmazeutische Biologie, Philipps-Universität Marburg, Deutschhausstr. 17A, 35037 Marburg, Germany.
Schneider
Bernd
B
Petersen
Maike
M
D049936:Anthocerotophyta / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis; D018929:Cell Culture Techniques; D002934:Cinnamates / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D003470:Culture Media; D053630:Depsides; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009005:Monosaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013997:Time Factors; D000097123:Rosmarinic Acid
D002241:Carbohydrates; D002934:Cinnamates; D003470:Culture Media; D053630:Depsides; D009005:Monosaccharides; D000097123:Rosmarinic Acid; C501718:3'-O-beta-D-glucopyranosyl rosmarinic acid