title: TANMEI/EMB2757 encodes a WD repeat protein required for embryo development in Arabidopsis.
abstract: We identified the Arabidopsis (Arabidopsis thaliana) tanmei/emb2757 (tan) mutation that causes defects in both embryo and seedling development. tan mutant embryos share many characteristics with the leafy cotyledon (lec) class of mutants in that they accumulate anthocyanin, are intolerant of desiccation, form trichomes on cotyledons, and have reduced accumulation of storage proteins and lipids. Thus, TAN functions both in the early and late phases of embryo development. Moreover, the TAN and LEC genes interact synergistically, suggesting that they do not act in series in the same genetic pathway but, rather, that they have overlapping roles during embryogenesis. tan mutants die as embryos, but immature mutant seeds can be germinated in culture. However, tan mutant seedlings are defective in shoot and root development, their hypocotyls fail to elongate in the dark, and they die as seedlings. We isolated the TAN gene and showed that the predicted polypeptide has seven WD repeat motifs, suggesting that TAN forms complexes with other proteins. Together, these results suggest that TAN interacts with other proteins to control many aspects of embryo development.
authors.affiliation: Section of Plant Biology, College of Biological Sciences, University of California, Davis, 95616, USA.
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Yamagishi
authors.forename: Noriko
authors.initials: N
authors.lastname: Nagata
authors.forename: Kelly Matsudaira
authors.initials: KM
authors.lastname: Yee
authors.forename: Siobhan A
authors.initials: SA
authors.lastname: Braybrook
authors.forename: Julie
authors.initials: J
authors.lastname: Pelletier
authors.forename: Shozo
authors.initials: S
authors.lastname: Fujioka
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yoshida
authors.forename: Robert L
authors.initials: RL
authors.lastname: Fischer
authors.forename: Robert B
authors.initials: RB
authors.lastname: Goldberg
authors.forename: John J
authors.initials: JJ
authors.lastname: Harada
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D036226:Seedlings; D012639:Seeds / Q000033:anatomy & histology / Q000196:embryology / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; C507146:TAN protein, Arabidopsis