Rapid separation and determination of aconitine alkaloids in traditional Chinese herbs by capillary electrophoresis using 1-butyl-3-methylimidazoium-based ionic liquid as running electrolyte.
A novel and very simple capillary electrophoretic method for analyzing aconitine components in Aconitum plants was developed using 1-butyl-3-methylimidazoium tetrafluoroborate (1B-3MI-TFB)-based ionic liquid as running electrolyte solution for the first time. The optimum conditions were 35 mM 1B-3MI-TFB solution (pH 8.50) and 15 kV applied voltage. The detection was performed at 254 nm. Aconitine, meaconitine and hypaconitine in Aconitum plants were separated and identified within 5 min. The recoveries were 91.0-103.0% for hypaconitine, 92.8-96.2% for aconitine and 96.0-106.6% for mesaconitine, respectively. Compared with other methods, the analytical time was decreased 4-8-fold and the effect of Joule heating was weaker because the current was smaller.
Qi
Shengda
S
Department of Chemistry, Lanzhou University, People's Republic of China.
Cui
Shuya
S
Cheng
Yuqiao
Y
Chen
Xingguo
X
Hu
Zhide
Z
D000157:Aconitine / Q000032:analysis*; D000470:Alkaloids / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D004573:Electrolytes; D007093:Imidazoles; D000157:Aconitine