title: [Identification of markers linked to resistance locus of Marssonina leaf spot in poplars by bulked segregant analysis(BSA)].
abstract: DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis(BSA). The bulks consisted of individual with a extreme phenotype taken from a population of 91 F1 clones,which is a progeny of Populus deltoides Bartr.cv."Lux"(I-69/55)(Resistance) and P.euramericana cv.I-45(Susceptible). Out of 114 RAPD primers, four markers showed polymorphisms between the resistance-bulk and the susceptible-bulk.By using selective genotype linkage analysis,OPAI17-1550 and OPAI13-900 were found linked to the resistance locus. The genetic distances between the two markers and the resistance locus were 29.9cM and 37.4cM,respectively.
authors.affiliation: The Key Laboratory of Forest Tree Genetic Engineering, Nanjing Forestry University, Nanjing,210037, China. zhangbo1978@hotmail.com
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Min-ren
authors.initials: MR
authors.lastname: Huang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhuge
authors.forename: Zheng-min
authors.initials: ZM
authors.lastname: Han
authors.forename: Tong-ming
authors.initials: TM
authors.lastname: Yin
authors.forename: Hui-xin
authors.initials: HX
authors.lastname: Pan
authors.forename: Li-huang
authors.initials: LH
authors.lastname: Zhu
authors.forename: Rong-ling
authors.initials: RL
authors.lastname: Wu
authors.forename: Ming-xiu
authors.initials: MX
authors.lastname: Wang