[Chromosome STR genetic markers in paternity identification].
OBJECTIVE
To explore the application of paternity identification by chromosome STR genetic markers (short tandem repeat).


METHODS
The paternity testings in 468 cases were routinely carried out using amphibian STR Profiler plus and Cofiler PCR amplification kits. When STR exclusions were found, HLA system and other blood groups were detected by molecular typing and loci of PowerPlex 16 system. STR loci were genotyped. If necessary, the genotyping of Y chromosome specific STR, X chromosome specific STR and HLA allelic sequencing were added.


RESULTS
W of 408 cases of paternity was more than 0.95, and that of 350 cases was more than 0. 9995. W of 60 cases of unrelated individuals was less than 0.8, and that of another 48 cases was less than 0.27. By Chi-Square test, there was significant difference (P < 0.001) in the entire-same allelic genes and the entire-different allelic genes and no significant difference (P > 0.05) in the half-same between allelic genes the two groups.


CONCLUSIONS
It is effective to identify the paternity by STR genetic markers. The pair is related when the locus number of entirely-different allelic genes is less than 1 or that of the entirely-same allelic genes is more than 6.
Li
Jing
J
Department of Forensic Medicine, Xiangya School of Medicine, Central South University, Changsha 410078, China. tzc9065@hotmail.com
D016172:DNA Fingerprinting; D005260:Female; D005554:Forensic Medicine; D005787:Gene Frequency; D005819:Genetic Markers; D006680:HLA Antigens / Q000235:genetics; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010334:Paternity; D011110:Polymorphism, Genetic
D005819:Genetic Markers; D006680:HLA Antigens