title: [Purification and properties of recombinant Erwinia carotovora L-asparaginase expressed in E.coli cells].
abstract: The method of purification Erwinia carotovora recombinant L-asparaginase, expressed in E.coli, including ultrasonic disintegration of biomass, fractionation ammonium sulfate and column chromatography on CM- or SP-Sepharose has been developed. According to SDS-PAAGE the enzyme preparation was homogeneous, its specific activity and yield consist respectively about 620 IU/mg of protein and 75%. Physical-chemical and structural properties of recombinant Erwinia carotovora L-asparaginase are similar to the enzymes from the wild strains Erwinia carotovora and recombinant L-asparaginase Erwinia chrysanthemi.
authors.forename: A A
authors.initials: AA
authors.lastname: Borisova
authors.forename: M A
authors.initials: MA
authors.lastname: El'darov
authors.forename: A A
authors.initials: AA
authors.lastname: Zhgun
authors.forename: S S
authors.initials: SS
authors.lastname: Aleksandrova
authors.forename: N M
authors.initials: NM
authors.lastname: Omel'ianiuk
authors.forename: B N
authors.initials: BN
authors.lastname: Sokov
authors.forename: T T
authors.initials: TT
authors.lastname: Berezov
authors.forename: N N
authors.initials: NN
authors.lastname: Sokolov
mesh_terms: D001215:Asparaginase / Q000096:biosynthesis / Q000302:isolation & purification*; D002847:Chromatography, Agarose; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D016973:Pectobacterium carotovorum / Q000201:enzymology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D001215:Asparaginase