Chlamydia trachomatis variant with nonfusing inclusions: growth dynamic and host-cell transcriptional response.
We compared growth rate and host-cell transcriptional responses of a Chlamydia trachomatis variant strain and a prototype strain. Growth dynamics were estimated by 16S rRNA level and by inclusion-forming units (IFUs) at different times after infection in HeLa cells. When inoculated at the same multiplicity of infection and observed 24-48 h after infection, the variant 16S rRNA transcriptional level was 3%-4% that of the prototype, and the IFUs of the variant strain were 0.1%-1% those of the prototype. Specific host-cell transcriptional responses to the variant were identified in a global-expression microarray in which variant strain-infected cells were compared with mock-infected and prototype strain-infected cells. In variant strain-infected cells, 47% (16/34) of specifically induced host genes were related to immunity and 32% (8/25) of specifically suppressed genes were related to lipid metabolism. The variant strain grew significantly more slowly and induced a modified host-cell transcriptional response, compared with the prototype strain.
Xia
Minsheng
M
Division of Infectious Diseases, Department of Medicine, University of Washington, Seattle, USA.
Suchland
Robert J
RJ
Bumgarner
Roger E
RE
Peng
Tao
T
Rockey
Daniel D
DD
Stamm
Walter E
WE
D002692:Chlamydia trachomatis / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D005786:Gene Expression Regulation; D014644:Genetic Variation; D006367:HeLa Cells / Q000276:immunology / Q000382:microbiology; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis; D008561:Membrane Fusion; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D008055:Lipids; D011506:Proteins; D012336:RNA, Ribosomal, 16S