Presence of Chlamydia pneumoniae in patients with and without atherosclerosis.
Data published over the past decade show that Chlamydia pneumoniae is likely associated with the development of atherosclerosis. The aim of this study was to ascertain whether C. pneumoniae infections occur more frequently in patients with atherosclerosis than in healthy subjects. A total of 517 persons were studied. Serum samples, leukocytes, and tissue samples were assayed for the presence of C. pneumoniae-specific IgG and IgA antibodies and C. pneumoniae DNA. C. pneumoniae DNA was found in renal, iliac, and brachial vessels, but it was not detected in radial arteries. C. pneumoniae DNA was found most often in directional coronary atherectomy tissue specimens (11/41, 26.8%), but it was also found in the leukocytes of 14.9% (28/188) of patients with atherosclerosis and 24.6% (28/114) of patients without atheroma changes in vessels. Specific IgG and IgA antibodies were present in 63.8 and 49.9% of atheroma patients, respectively. The prevalence of C. pneumoniae antibodies differs significantly in patients with and without atherosclerosis (for IgG, p=0.002, and for IgA, p=0.006). The identification of persons with chlamydial infection of atherosclerotic arteries necessitates the examination of vascular tissues obtained during revascularization procedures. Serological investigation alone cannot identify individuals with vascular chlamydial infections. Detection of C. pneumoniae DNA in peripheral blood mononuclear cells does not seem to be the exclusive marker of persistent vascular infection. A more easily accessible parameter that allows prediction of chlamydial vascular infection is required.
Podsiadły
E
E
National Institute of Hygiene, Laboratory of Rickettsiae, Chlamydiae and Enzotic Spirochetes, Chocimska 24, 00-791, Warsaw, Poland. epodsiadly@pzh.gov.pl
Przyłuski
J
J
Kwiatkowski
A
A
Kruk
M
M
Wszoła
M
M
Nosek
R
R
Rowiński
W
W
Ruzyłło
W
W
Tylewska-Wierzbanowska
S
S
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D001161:Arteriosclerosis / Q000150:complications* / Q000276:immunology / Q000382:microbiology; D002339:Carotid Arteries / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D002690:Chlamydia Infections / Q000097:blood / Q000150:complications* / Q000276:immunology; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification* / Q000472:pathogenicity; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000737:chemistry / Q000382:microbiology* / Q000473:pathology; D008297:Male; D008875:Middle Aged
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial