title: Selective reduction of multifetal pregnancy using high-intensity focused ultrasound in the rabbit model.
abstract: OBJECTIVE
  High-order multifetal pregnancies carry a significant risk of obstetric complications and poor pregnancy outcome. Selective reduction has traditionally been performed using transabdominal and transvaginal ultrasound-guided intracardiac injection of potassium chloride. We have previously shown that high-intensity focused ultrasound (HIFU) can create a coagulative tissue necrosis in the sheep fetus. The objective of this study was to investigate the feasibility of non-invasive selective fetal reduction using HIFU in a rabbit model.
  
  
  METHODS
  A protocol for HIFU-induced tissue coagulation was developed in the rabbit model. The fetal heart was targeted with ultrasound-guided tissue ablation by a HIFU beam. Five time-mated does between 20-29 days' gestation underwent transabdominal fetal cardiac ablation in a total of 11 fetuses. The HIFU system consisted of a 7-MHz high-power transducer, operated at 2000 W/cm2. The fetal heart rate was observed using real-time ultrasound with Doppler flow velocimetry. All lesions were assessed macroscopically and by histological analysis.
  
  
  RESULTS
  Severe bradycardia leading to asystole was observed in all targeted fetuses with ultrasound examination. Dissection of fetuses demonstrated a necrotic intrathoracic lesion similar in size to the HIFU focus (approximately 1 x 9 mm). None of the surrounding fetuses was found to have bradycardia during the procedure or a macroscopic lesion on dissection.
  
  
  CONCLUSIONS
  In this pilot study HIFU seems promising to ablate even highly vascularized tissue in the fetus.
authors.affiliation: Department of Obstetrics and Gynecology, University of Washington, Seattle, WA 98195-6460, USA. bettina@u.washington.edu
authors.forename: B
authors.initials: B
authors.lastname: Paek
authors.forename: J
authors.initials: J
authors.lastname: Foley
authors.forename: V
authors.initials: V
authors.lastname: Zderic
authors.forename: F
authors.initials: F
authors.lastname: Starr
authors.forename: L E
authors.initials: LE
authors.lastname: Shields
authors.forename: S
authors.initials: S
authors.lastname: Vaezy
mesh_terms: D000818:Animals; D005240:Feasibility Studies; D005260:Female; D023421:Models, Animal; D011247:Pregnancy; D018607:Pregnancy Reduction, Multifetal / Q000295:instrumentation / Q000379:methods*; D011817:Rabbits; D014464:Ultrasonic Therapy / Q000379:methods*