[Screen selection of target genes regulated by HSF1 in heat shock response from myocardium of gene knockout mice].
OBJECTIVE
To screen select target genes regulated by heat shock transcription factor 1 (HSF1 ) in heat shock response from myocardium of HSF1 gene knockout mice using cDNA chip.


METHODS
HSF1 knockout mice were subjected to heat shock treatment (42 degrees C for 15 minutes, and recovered for 3 hours), and cDNA chip containing 15 000 mouse cDNAs were employed to investigate the expression of genes regulated by HSF1 in the myocardium. Then 4 of the differentially expressed genes in the myocardium of HSF1 knockout mice were further confirmed by RT-PCR. The promoter regions of the differentially expressed genes were analysed by TESS software to find HSE.


RESULTS
Three hundred and ninety-eight genes were down - regulated in the myocardium of HSF1 knockout mice in heat shock response,with 173 known. Whereas 641 genes were up-regulated in the myocardium, with 235 known. The 5 down-regulated genes and 6 up-regulated ones were shown to contain HSE in their promoter regions.


CONCLUSIONS
In heat shock response, expression of multiple genes is regulated directly or indirectly by HSF1.
Liu
Ying
Y
Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Yuan
Can
C
Zhang
Hua-Li
HL
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D000076249:Heat Shock Transcription Factors; D018869:Heat-Shock Response / Q000235:genetics*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009206:Myocardium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D014157:Transcription Factors