title: Effect of cooling rates on the cold hardiness and cryoprotectant profiles of locust eggs.
abstract: To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eggs. Egg survival rate increased with decreasing cooling rate. The concentration of cryoprotectants (myo-inositol, trehalose, mannitol, glycerol, and sorbitol) increased in non-acclimated eggs, but varied significantly in response to different cooling rates in acclimated eggs. The acclimation process (5 degrees C for 3 days) did not increase eggs resistance to quick cooling ("plunge" cooling and 0.8 degrees C min(-1)). Earlier stage embryos were much more sensitive than later stage embryos to the same cooling rates. Time spent at subzero temperatures also had a strong influence on egg survival.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, the Chinese Academy of Sciences, 25 Beisihuanxi Road, Haidian District, Beijing 100080, PR China.
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Wang
authors.forename: Le
authors.initials: L
authors.lastname: Kang
mesh_terms: D000220:Adaptation, Biological / Q000187:drug effects / Q000502:physiology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods* / Q000592:standards; D003451:Cryoprotective Agents / Q000032:analysis / Q000494:pharmacology; D003627:Data Interpretation, Statistical; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian; D005260:Female; D048188:Locusta migratoria / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D008297:Male; D010063:Ovum / Q000502:physiology*; D013402:Sugar Alcohols / Q000032:analysis / Q000494:pharmacology; D013997:Time Factors
chemical_list: D003451:Cryoprotective Agents; D013402:Sugar Alcohols