title: Data standards for proteomics: mitochondrial two-dimensional polyacrylamide gel electrophoresis data as a model system.
abstract: Proteomics has emerged as a major discipline that led to a re-examination of the need for consensus and a nationally sanctioned set of proteomics technology standards. Such standards for databases and data reporting may be applied to two-dimensional polyacrylamide gel electrophoresis (2D PAGE) technology as a pilot project for assessing global and national needs in proteomics, and the role of the National Institute of Standards and Technology (NIST) and other similar standards and measurement organizations. The experience of harmonizing the heterogeneous data included in the Protein Data Bank (PDB) provides a paradigm for technology in an area where significant heterogeneity in technical detail and data storage has evolved. Here we propose an approach toward standardizing mitochondrial 2D PAGE data in support of a globally relevant proteomics consensus.
authors.affiliation: Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA. vravi@nist.gov
authors.forename: Veerasamy
authors.initials: V
authors.lastname: Ravichandran
authors.forename: Gregory B
authors.initials: GB
authors.lastname: Vasquez
authors.forename: Sudhir
authors.initials: S
authors.lastname: Srivastava
authors.forename: Mukesh
authors.initials: M
authors.lastname: Verma
authors.forename: Emanuel
authors.initials: E
authors.lastname: Petricoin
authors.forename: Joshua
authors.initials: J
authors.lastname: Lubell
authors.forename: Ram D
authors.initials: RD
authors.lastname: Sriram
authors.forename: Peter E
authors.initials: PE
authors.lastname: Barker
authors.forename: Gary L
authors.initials: GL
authors.lastname: Gilliland