[Effect of selenium on expression of P38 mitogen-activated protein kinase and monocyte chemoattractant protein-1 in diabetic endothelial cells].
OBJECTIVE
To study the relationship between P38MAPK and MCP-1 in diabetic HUVEC and the mechanism of anti-atherosclerosis of selenium.


METHODS
HUVEC were treated with high concentration of glucose, advanced glycosylation end products (AGE), high concentration of insulin or H(2)O(2) with or without pre-treatment with SB203580 (P38MAPK specific inhibitor) or selenium. The expression of phospho-P38MAPK and MCP-1 in HUVEC was detected by Western blot or RT-PCR, respectively.


RESULTS
High concentration of glucose, AGE, high concentration of insulin and H(2)O(2) can activate P38MAPK and increase the expression of MCP-1 in HUVEC. The expression of MCP-1 was inhibited by SB203580. Selenium inhibited the activation of P38MAPK and reduced the expression of MCP-1.


CONCLUSIONS
P38MAPK is an upstream signaling molecule of MCP-1. P38MAPK may be one of the initiating signals of diabetic atherosclerosis. Selenium can inhibit the expression of MCP-1 by repressing P38MAPK signaling pathway and therefore prevent the development of atherosclerosis.
Li
Yan-bo
YB
Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. liyanbo65@163.com
Li
Wei-min
WM
Han
Jun-yong
JY
D015153:Blotting, Western; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D017127:Glycation End Products, Advanced / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012643:Selenium / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*
D018932:Chemokine CCL2; D017127:Glycation End Products, Advanced; D007093:Imidazoles; D007328:Insulin; D011725:Pyridines; D006861:Hydrogen Peroxide; D048051:p38 Mitogen-Activated Protein Kinases; D012643:Selenium; D005947:Glucose; C093642:SB 203580