The HLDA8 blind panel: findings and conclusions.
There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity.
Swart
Bernadette
B
Child Health Research Institute, Women's and Children's Hospital and Department of Paediatrics, University of Adelaide, 72 King William Rd, North Adelaide, 5006, Australia.
Salganik
Mikhail P
MP
Wand
Matthew P
MP
Tinckam
Kathryn
K
Milford
Edgar L
EL
Drbal
Karel
K
Angelisova
Pavla
P
Horejsi
Vaclav
V
Macardle
Peter
P
Bailey
Sheree
S
Hollemweguer
Enoc
E
Hodge
Greg
G
Nairn
Judith
J
Millard
Debbrah
D
Dagdeviren
Attila
A
Dandie
Geoffrey W
GW
Zola
Heddy
H
D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D007120:Immunochemistry
D000906:Antibodies; D015703:Antigens, CD; D000954:Antigens, Surface