title: Development of a Bacillus sphaericus tablet formulation and its evaluation as a larvicide in the biological control of Culex quinquefasciatus.
abstract: This study aimed to analyze the final fermentation culture of Bacillus sphaericus 2362, standardize it and develop an active tablet formulation for use in urban mosquito breeding sites. It was performed in three phases: analysis and standardization of a B. sphaericus fermented culture; physical, chemical, and biological analysis of the active powder (solubility, residual humidity, particle size, resting angle, flowing off time, compacted density, and biological activity against Culex quinquefasciatus larvae); and the development of fast-disintegrating tablets. Five formulations with differing compositions were developed and a UV protector was added to the selected formulation. The formulation products with or without UV protector, as well as the active powder caused 100% larval mortality from 1 day to 2 months after a single treatment under simulated field conditions. These results show that the UV protector does not affect the initial larvicide activity of B. sphaericus, nor its persistence over a period of two months.
authors.affiliation: Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, 50740-521 Recife, PE, Brazil. flavia.morais@lafepe.pe.gov.br
authors.forename: Flávia P Morais de
authors.initials: FP
authors.lastname: Medeiros
authors.forename: Maria Alice Varjal de Melo
authors.initials: MA
authors.lastname: Santos
authors.forename: Leda
authors.initials: L
authors.lastname: Regis
authors.forename: Eugênia M Maranhão
authors.initials: EM
authors.lastname: Rios
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Rolim Neto
mesh_terms: D000818:Animals; D001407:Bacillus; D003465:Culex; D007814:Larva; D007928:Lethal Dose 50; D010572:Pest Control, Biological / Q000379:methods*; D013607:Tablets; D013997:Time Factors
chemical_list: D013607:Tablets