[Effects of bone marrow endothelial cell conditioned medium on the expansion of mature megakaryocytes and CFU-Meg in vitro].
OBJECTIVE
To investigate the effects of serum-free bone marrow endothelial cell conditioned medium (E-CM) and serum-free bone marrow fibroblast conditioned medium (F-CM) on the expansion of mature megakaryocytes and CFU-Meg .


METHODS
E-CM and F-CM were collected and added in liquid culture system respectively.


RESULTS
E-CM and F-CM significantly promoted the expansion of mature megakaryocytes and CFU-Meg in liquid culture system. The effect of E-CM on the expansion of CFU-Meg was better than the effect of F-CM, While the effect of E-CM on the expansion of mature megakaryocytes was inferior to the effect of F-CM.


CONCLUSIONS
E-CM can be used to expand CFU-Meg while F-CM can be used to expand the mature megakaryocytes in vitro.
Huang
Yan-Hong
YH
Research Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
Wang
Qi-Ru
QR
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000378:metabolism; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D008297:Male; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice
D003115:Colony-Stimulating Factors; D017077:Culture Media, Conditioned