Statistical methods for comparing comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry results: metabolomic analysis of mouse tissue extracts.
The potential for the utilization of GC x GC-time-of-flight (TOF) MS for high-resolution metabolomics studies is discussed, with the implementation of some statistical comparisons for biomarker detection. Metabolite profiles from NZO obese mice versus BL/6 control mice are compared and contrasted using a number of chromatogram comparison routines, including direct chromatogram comparisons, chromatogram subtraction and averaging routines, as well as a method for generating relative weighted peak surface difference chromatograms, and a more conventional Student's t-test statistical approach.
Shellie
Robert A
RA
Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
Welthagen
Werner
W
Zrostliková
Jitka
J
Spranger
Joachim
J
Ristow
Michael
M
Fiehn
Oliver
O
Zimmermann
Ralf
R
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D051379:Mice