[Preliminary study on anti-tumor function of resveratrol and its immunological mechanism].
OBJECTIVE
To explore the immunological mechanism of the anti-tumor function of resveratrol (Res) through in vitro or vivo experiments.


METHODS
The tumor cell growth repression rate was measured by MTT colorimetry. Flow cytometry was used to analyze the cell cycle of Hep2 cells (laryngeal squamous cancer cell line). The immune function and cytokine levels in the serum of the tumor-bearing mice were determined by MTT colorimetry, hemolysis spectrophotography, improved Mayer's method and ELISA.


RESULTS
Res inhibited the tumor cell growth and enhanced the apoptosis of tumor cells in time-concentration-dependent manners showing the phenomenon of obvious G(0)/G(1) blocking and apoptotic peak. The maximal tumor inhibition rate came up to 42.76%. Furthermore, Res improved function of T, B lymphocytes, killing activity of NK cells, release of antibodies, and the total complement activity in serum. It also increased contents of IL-2 and TGF-beta1 but reduced that of IL-8 and VEGF.


CONCLUSIONS
Res can not only affect tumor cells directly but also exert anti-tumor efficiency through reinforcing cell-mediated, humoral immune response and accommodating lymphocytes to secrete cytokines.
Li
Tan
T
Department of Immunology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. litan2002@eyou.com
Sheng
Li
L
Fan
Gui-xiang
GX
Yuan
Yu-kang
YK
Li
Tong
T
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000188:drug therapy; D051379:Mice; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D000970:Antineoplastic Agents; D007376:Interleukin-2; D016209:Interleukin-8; D013267:Stilbenes; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D000077185:Resveratrol