Quantification of a glucocorticoid receptor in sea bass (Dicentrarchus labrax, L.) reared at high stocking density.
To assess the effects of environmental stress conditions on fish, the examination of a suite of biomarkers, including endocrine parameters, has been suggested. In teleosts, glucocorticoids, including cortisol and corticosterone, are known to mediate stress response. Inside the cell, they bind to a high-affinity cytosolic glucocorticoid receptor (GR), which acts as ligand-dependent transcription factor to control and regulate gene expression. Receptor number or affinity may directly influence the degree of reactivity of target cells. GR transcripts have been cloned and characterized in different fish species, but no studies, to date, are available on the quantification of GR in sea bass (Dicentrarchus labrax, L.), although this is one of the most important species in Mediterranean aquaculture. These considerations prompted our interest in cloning the GR in this species and investigating the impact of long-term exposure to crowding stress on GR expression in the liver of sea bass. Our data clearly demonstrate that a high rearing density stress affects GR mRNA, whose abundance in the liver decreased inversely with blood cortisol levels.
Terova
Genciana
G
Department of Biotechnology and Molecular Sciences, University of Insubria, Via J.H. Dunant, 3, 21100 Varese, Italy. genciana.terova@uninsubria.it
Gornati
Rosalba
R
Rimoldi
Simona
S
Bernardini
Giovanni
G
Saroglia
Marco
M
D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000097:blood / Q000235:genetics*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000502:physiology*; D006854:Hydrocortisone / Q000097:blood / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000097:blood / Q000235:genetics*; D013312:Stress, Physiological / Q000097:blood / Q000235:genetics
D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D006854:Hydrocortisone