title: Amplified fragment length polymorphism to detect clonal diversity and distribution of Mycobacterium avium subspecies paratuberculosis in selected Minnesota dairy cattle.
abstract: The molecular ecology of Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease, is not well understood in the United States. In this study, a DNA fingerprinting method, amplified fragment length polymorphism (AFLP), was used to subtype the pathogen and assess the clonal diversity of MAP in Minnesota dairy herds. Fifty-six fecal culture test-positive isolates from various Minnesota counties and culture dates were analyzed in this study. The AFLP identified 11 profiles with 50% of isolates representing 1 major profile. The major profile was distributed across the state. The genetic diversity of bovine MAP clones in Minnesota based on AFLP analysis of this data appears to be relatively low.
authors.affiliation: Centers for Disease Control and Prevention, Minneapolis, MN 55414, USA.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Kiehnbaum
authors.forename: Alongkorn
authors.initials: A
authors.lastname: Amonsin
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Wells
authors.forename: Vivek
authors.initials: V
authors.lastname: Kapur
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D008910:Minnesota; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004269:DNA, Bacterial