title: Gene transfection into HeLa cells by vesicles containing cationic peptide lipid.
abstract: Lipid vesicles are potentially useful as microcapsules for drug and/or gene delivery. We developed cationic lipid vesicles consisting mainly of sorbitan monooleate (Span 80) and cationic peptide lipid (CPL), and evaluated the CPL vesicles as gene transfection vectors. The optimum CPL concentration for gene transfection into HeLa cells was found to be 20 wt % of total lipid, and such CPL vesicles did not exhibit significant cytotoxicity. Co-culture of Poly-L-lysine and plasmids prior to making CPL vesicle-plasmid complexes was effective. Lipofection using LipofectAMINE was suppressed in 10% serum-supplemented medium. The transfection efficiency of 20 wt % CPL vesicles, however, was not affected by serum in the medium when plasmids were treated with poly-L-lysine.
authors.affiliation: Faculty of Agriculture, Ehime University, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Ohama
authors.forename: Yuji
authors.initials: Y
authors.lastname: Heike
authors.forename: Takuya
authors.initials: T
authors.lastname: Sugahara
authors.forename: Kozue
authors.initials: K
authors.lastname: Sakata
authors.forename: Norikazu
authors.initials: N
authors.lastname: Yoshimura
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hisaeda
authors.forename: Mami
authors.initials: M
authors.lastname: Hosokawa
authors.forename: Shigemitsu
authors.initials: S
authors.lastname: Takashima
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kato
mesh_terms: D001769:Blood; D003470:Culture Media; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D014162:Transfection
chemical_list: D003470:Culture Media; D008055:Lipids; D010455:Peptides