Extracellular matrix degrading enzymes at the prostasome surface.
Prostasomes, prostatic secretory vesicles found in human ejaculates, were analyzed to verify the existence at their surfaces of enzymes involved in the degradation of the extracellular matrix. Findings were compared with those of prostasomes isolated from two human adenocarcinoma cell lines that reflect clinical features and molecular pathways of androgen-insensitive and hormone-responsive prostate cancer. Our aim was to determine whether neoplastic transformation is accompanied by changes of glycosidase and protease activities. Our results show that decreases of dipeptidyl peptidase IV and increases of urokinase plasminogen activator and cathepsin B are consistent with the clinical features of the cell lines, whereas increases of glycosidase activities seem to be of scarce biological significance.
Bellezza
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Dipartimento Medicina Sperimentale Scienze Biochimiche, Sezione Biochimica Cellulare, Perugia, Italy.
Aisa
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MC
Palazzo
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Costanzi
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Mearini
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Minelli
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D002401:Cathepsin B / Q000378:metabolism; D045744:Cell Line, Tumor; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D008297:Male; D010447:Peptide Hydrolases / Q000378:metabolism; D010457:Peptidoglycan / Q000378:metabolism; D022142:Secretory Vesicles / Q000201:enzymology*; D012661:Semen / Q000166:cytology* / Q000201:enzymology*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D010457:Peptidoglycan; D006026:Glycoside Hydrolases; D010447:Peptide Hydrolases; D018819:Dipeptidyl Peptidase 4; D014568:Urokinase-Type Plasminogen Activator; D002401:Cathepsin B