title: Rapid determination of methyl salicylate, a plant-signaling compound, in tomato leaves by direct sample introduction and thermal desorption followed by GC-MS.
abstract: Many plants infested by herbivores or viruses can rapidly produce and accumulate a plant-signaling compound, methyl salicylate (MeSA), in their leaves to activate disease resistance. In the present work, a simple, rapid, and sensitive method was developed for the determination of MeSA in tomato leaves by direct sample introduction and thermal desorption followed by GC-MS. Results show that the proposed method has a low detection limit (0.08 ng mg(-1)) and good precision (RSD = 8.9%). The present method was applied to the investigation of tomato plant defense response to tobacco mosaic virus (TMV) by rapid analysis of volatile compounds in plant leaves. It was found that tomato plants can produce large amounts of MeSA as a defense response to TMV. This indicates that MeSA may be a plant-signaling compound in tomato plant defense response to TMV.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Ji
authors.initials: J
authors.lastname: Qian
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhu
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yang
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000821:virology; D018515:Plant Leaves / Q000737:chemistry*; D012459:Salicylates / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors; D014027:Tobacco Mosaic Virus / Q000276:immunology
chemical_list: D012459:Salicylates; C033069:methyl salicylate