title: Tyl6, a novel Ty3/gypsy-like retrotransposon in the genome of the dimorphic fungus Yarrowia lipolytica.
abstract: The novel LTR retrotransposon Tyl6 was detected in the genome of the dimorphic fungus Yarrowia lipolytica. Sequence analysis revealed that this element is related to the well-known Ty3 element of Saccharomyces cerevisiae and, especially, to the recently described Tse3 retrotransposon of Saccharomyces exiguus and to the del1-like plant retrotransposons. Tyl6 is 5108 bp long, is flanked by two identical long terminal repeats (LTR), each of 276 bp, and its ORFs are separated by a -1 frameshift. Both ORFs are intact and deduced translation products display a significant similarity with those of previously described Ty3/gypsy retrotransposons. Distribution of Tyl6 among Y. lipolytica strains of different origins was also analysed. A single copy of the novel retrotransposon is present in some commonly used laboratory strains, which are derivatives of the wild-type isolate YB423-12, whereas other strains of independent origin are devoid of Ty16. No solo LTR of Tyl6 was detected in the analysed strains.
authors.affiliation: Institute of Microbiology, Dresden University of Technology, D-01062 Dresden, Germany.
authors.forename: Andriy
authors.initials: A
authors.lastname: Kovalchuk
authors.forename: Senam
authors.initials: S
authors.lastname: Senam
authors.forename: Stephan
authors.initials: S
authors.lastname: Mauersberger
authors.forename: Gerold
authors.initials: G
authors.lastname: Barth
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D014176:Protein Biosynthesis; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D018626:Retroelements / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D020079:Terminal Repeat Sequences; D025062:Yarrowia / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D018626:Retroelements; D012194:RNA-Directed DNA Polymerase