Molecular recognition of nucleic acids: coralyne binds strongly to poly(A).
The small molecule coralyne was found to bind preferentially and strongly to single-stranded poly(A) with an apparent association constant (Ka) of (1.8+/-0.3) x 10(6)M(-1). Binding of coralyne to poly(A) is predominantly enthalpically driven with a stoichiometry of one coralyne per four adenine bases. Poly(A) forms a coralyne dependent secondary structure with a melting temperature of 60 degrees C, for the conditions of our study.
Xing
Feifei
F
Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Song
Guangtao
G
Ren
Jinsong
J
Chaires
Jonathan B
JB
Qu
Xiaogang
X
D000241:Adenosine / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001600:Berberine Alkaloids / Q000378:metabolism*; D002942:Circular Dichroism; D046911:Macromolecular Substances; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D001600:Berberine Alkaloids; D046911:Macromolecular Substances; D011108:Polymers; D012333:RNA, Messenger; C069424:polyadenosine; C000666:coralyne; D000241:Adenosine