title: An aberrant autocrine activation of the platelet-derived growth factor alpha-receptor in follicular and papillary thyroid carcinoma cell lines.
abstract: Platelet-derived growth factor receptor (PDGFR) can bind to its ligand and consequently possess a kinase activity, and which is associated with the carcinogenesis of different cell types, including astrocytomas, oligodendrogliomas, and glioblastoma. In a cDNA microarray analysis, we observe the over-expressed mRNA of both PDGF-A and PDGF-alpha receptor in thyroid carcinoma cells. And the elevated protein expressions of PDGF-A and PDGF-alpha receptor in thyroid carcinoma cells were also confirmed by a Western blot analysis. The phosphorylation of PDGF-alpha receptor evaluated by an antibody against Tyr 720-phosphate was found in thyroid carcinoma cells. The tyrosine kinase activity of PDGF-alpha receptor was inhibited by tyrphostin AG1295 and showed a dose-dependent inhibition for the proliferation of thyroid carcinoma cells. These findings imply that autocrine activation of PDGF-alpha receptor plays a crucial role in the carcinogenesis of thyroid cells.
authors.affiliation: School of Medical Technology, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Kuei-Tien
authors.initials: KT
authors.lastname: Chen
authors.forename: Jen-Der
authors.initials: JD
authors.lastname: Lin
authors.forename: Miaw-Jene
authors.initials: MJ
authors.lastname: Liou
authors.forename: Hsiao-Fen
authors.initials: HF
authors.lastname: Weng
authors.forename: C Allen
authors.initials: CA
authors.lastname: Chang
authors.forename: Err-Cheng
authors.initials: EC
authors.lastname: Chan
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000235:genetics / Q000378:metabolism*; D000231:Adenocarcinoma, Papillary / Q000235:genetics / Q000378:metabolism*; D019898:Autocrine Communication; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D018076:DNA, Complementary; D004789:Enzyme Activation; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism; D020032:Tyrphostins / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D020032:Tyrphostins; C075278:platelet-derived growth factor A; D014443:Tyrosine; D020796:Receptor, Platelet-Derived Growth Factor alpha