A subpopulation of human B lymphocytes can express a functional Tissue Factor in response to phorbol myristate acetate.
Tissue Factor (TF,CD142) is a transmembrane glycoprotein that belongs to the cytokine receptor superfamily. This multifunctional protein is constitutively present in most tissues. Among circulating blood cells, TF expression is known to be restricted to monocytes which do not constitutively produce TF but express TF in response to various stimuli. Here, we report that highly purified B lymphocytes can support a procoagulant activity (PCA) in response to phorbol myristate acetate (PMA). Using flow cytometry (FCM), we observed that a subpopulation of B lymphocytes (CD19+) can express TF and anionic phospholipids in response to PMA. TF protein was identified and characterized by immunofluorescence, immunocytochemistry and electronic microscopy. Using RT-PCR, we identified the presence of TF mRNA in response to PMA. In conclusion, B lymphocytes are a potential source of functional TF in human.
Mechiche
Houria
H
Laboratoire Central d'Hématologie, CHU de Reims, Avenue du Général Koenig, 51092 Reims Cedex, France.
Cornillet-Lefebvre
Pascale
P
Nguyen
Philippe
P
D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D002478:Cells, Cultured; D003029:Coagulants / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009000:Monocytes / Q000166:cytology; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013925:Thromboplastin / Q000096:biosynthesis*; D013997:Time Factors
D003029:Coagulants; D012333:RNA, Messenger; D013925:Thromboplastin; D013755:Tetradecanoylphorbol Acetate