title: Expression of serum amyloid A, in normal, dysplastic, and neoplastic human colonic mucosa: implication for a role in colonic tumorigenesis.
abstract: Serum amyloid A (SAA) is an acute phase reactant, whose level in the blood is elevated in response to trauma, infection, inflammation, and neoplasia. Elevated levels of SAA in the serum of cancer patients were suggested to be of liver origin rather than a tumor cell product. The role of SAA in human malignancies has not been elucidated. We investigated the expression of SAA at various stages of human colon carcinoma progression. Nonradioactive in situ hybridization applied on paraffin tissue sections from 26 colon cancer patients revealed barely detected SAA mRNA expression in normal looking colonic epithelium. Expression was increased gradually as epithelial cells progressed through dysplasia to neoplasia. Deeply invading colon carcinoma cells showed the highest levels of SAA. Expression was also found in colon carcinoma metastases. Cells of lymphoid follicles of the intestinal wall, inflammatory cells, ganglion cells, and endothelial cells, also expressed SAA mRNA. Immunohistochemical staining revealed SAA protein expression that colocalized with SAA mRNA expression. RT-PCR analysis confirmed the expression of the SAA1 and SAA4 genes in colon carcinomas, expression that was barely detectable in normal colon tissues. These findings indicate local and differential expression of SAA in human colon cancer tissues and suggest its role in colonic tumorigenesis.
authors.affiliation: Department of Oncology, Hadassah-Hebrew University Medical Center, Mount Scopus, Jerusalem 91240, Israel.
authors.forename: Orit
authors.initials: O
authors.lastname: Gutfeld
authors.forename: Diana
authors.initials: D
authors.lastname: Prus
authors.forename: Zvi
authors.initials: Z
authors.lastname: Ackerman
authors.forename: Shira
authors.initials: S
authors.lastname: Dishon
authors.forename: Reinhold P
authors.initials: RP
authors.lastname: Linke
authors.forename: Mark
authors.initials: M
authors.lastname: Levin
authors.forename: Simcha
authors.initials: S
authors.lastname: Urieli-Shoval
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000556:secondary; D018256:Adenomatous Polyps / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D003106:Colon / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary; D008198:Lymph Nodes / Q000378:metabolism; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009852:Omentum / Q000378:metabolism; D010534:Peritoneal Neoplasms / Q000378:metabolism / Q000556:secondary; D011230:Precancerous Conditions / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000685:Serum Amyloid A Protein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D000685:Serum Amyloid A Protein