[Application of real-time fluorescence quantitative polymerase chain reaction techniques for prenatal diagnosis of Down syndrome].
OBJECTIVE
To investigate the value of real-time fluorescence quantitative PCR in diagnosis of Down syndrome with uncultured amniotic cells.


METHODS
The uncultured amniocytes of 80 fetuses who were confirmed disomy 21 by chromosome analysis and 5 fetuses detected trisomy 21 and peripheral blood samples of 7 children diagnosed as Down syndrome were collected to extract gDNA and the real-time fluorescence quantitative PCR method was used to detect the original copies of Down syndrome critical region gene(3) (DSCR(3)) and GAPDH gene and then the ratio of DSCR(3)/GAPDH was calculated.


RESULTS
The PCR product ratios of DSCR(3) to GAPDH in trisomy 21 from amniocytes and peripheral blood were ranged from 1.64 to 1.98 while in the normal control the ratio was only from 0.46 to 1.30.


CONCLUSIONS
Real-time fluorescence quantitative PCR is a valuable method for rapid and accurate prenatal diagnosis of Down syndrome in uncultured amniotic cells.
Zheng
Ming-ming
MM
Department of Obstetrics and Gynecology, Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China.
Hu
Ya-li
YL
Xu
Zheng-feng
ZF
Wang
Zhi-qun
ZQ
Shi
Ting-qi
TQ
Ye
Xiao-dong
XD
Cui
Heng-mi
HM
D004314:Down Syndrome / Q000175:diagnosis*; D005260:Female; D005453:Fluorescence; D006801:Humans; D007621:Karyotyping; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*