[Determination of acrylamide in heated starchy food by liquid chromatography-electrospray ionization tandem mass spectrometry].
An isotope dilution liquid chromatographic-electrospray ionization tandem mass spectrometric method has been established for the determination of acrylamide in heated starchy food with a C18 analytical column and methanol-water containing 0.1% formic acid (2:98, v/v) as mobile phases. The clean-up of samples was performed on an Oasis HLB solid phase extraction cartridge. The calibration curve of acrylamide showed good linearity in the range of 10-500 microg/L with correlation coefficient of 0.9995. The detection limit of the method was 6 microg/kg (S/N = 3), and the limit of quantification was 20 microg/kg (S/N = 10). The average recoveries at three levels ranged from 96.8% to 97.4% (n = 5), and the relative standard derivations were lower than 10%.
Zhao
Rong
R
Beijing Center for Disease Prevention and Control, Beijing 100013, China.
Shao
Bing
B
Zhao
Jie
J
Wu
Yongning
Y
Wu
Guohua
G
Xue
Ying
Y
D020106:Acrylamide / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D006358:Hot Temperature; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
D020106:Acrylamide