title: Acute blood-brain barrier opening in experimentally induced focal cerebral ischemia is preferentially identified by quantitative magnetization transfer imaging.
abstract: Pathologic changes in brain tissue during and after stroke may lead to injury of the blood-brain barrier (BBB) and subsequent hemorrhagic transformation (HT). In a rat model of HT, the apparent diffusion coefficient of water, cerebral blood flow, relaxation times, T(1) and T(2), and magnetization transfer (MT) related parameters (T(1sat), K(for) and the MT ratio) were repetitively measured during 3 h of focal ischemia and 2 h of reperfusion (n = 8). Areas of BBB opening were identified by sequential assay of the transcapillary influx of Gd-diethylenetriaminepentaacetic acid (Gd-DTPA) by MRI and (14)C-alpha-aminoisobutyric acid (AIB) by quantitative autoradiography. Ischemia-injured regions of interest were identified from the MRI data and divided into those with and without BBB opening. Of the several MRI parameters measured, the T(1sat) in the caudate-putamen and preoptic area during ischemia and the first 2 h of reperfusion correlated best with the regional pattern of BBB opening observed thereafter. These data suggest that an ipsilateral/contralateral T(1sat) ratio > 1.6 demarcates leakage of small molecules such as Gd-DTPA and AIB across the BBB. As to clinical relevance, the quantitation of MT parameters in acute stroke may enable the early detection of areas of BBB opening and potential HT.
authors.affiliation: Department of Neurology, Henry Ford Health Sciences Center, Detroit, MI 48202, USA. knight@neurnis.neuro.hfh.edu
authors.forename: Robert A
authors.initials: RA
authors.lastname: Knight
authors.forename: Vijaya
authors.initials: V
authors.lastname: Nagesh
authors.forename: Tavarekere N
authors.initials: TN
authors.lastname: Nagaraja
authors.forename: James R
authors.initials: JR
authors.lastname: Ewing
authors.forename: Polly A
authors.initials: PA
authors.lastname: Whitton
authors.forename: Eric
authors.initials: E
authors.lastname: Bershad
authors.forename: Susan C
authors.initials: SC
authors.lastname: Fagan
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Fenstermacher
mesh_terms: D000208:Acute Disease; D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001812:Blood-Brain Barrier / Q000000981:diagnostic imaging / Q000473:pathology*; D001921:Brain / Q000098:blood supply / Q000000981:diagnostic imaging / Q000473:pathology; D002545:Brain Ischemia / Q000000981:diagnostic imaging / Q000473:pathology*; D003287:Contrast Media; D019786:Gadolinium DTPA; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
chemical_list: D003287:Contrast Media; D019786:Gadolinium DTPA