The effect of dimyristoylphosphatidylethanol on the lateral and rotational mobilities of liposome lipid bilayers.
The aim of this study was to provide the basis to further examine the mode of action of ethanol. Fluorescent probes reported to have different membrane mobilities were used to evaluate the effect of dimyristoylphosphatidylethanol (DMPEt) on the lateral and rotational mobilities of liposome lipid bilayers. An experimental procedure, based on the selective quenching of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups, was used. DMPEt increased the bulk lateral and rotational mobilities, and had a greater fluidizing effect on the outer than the inner monolayer. These effects of DMPEt on liposomes may be responsible for some, but not all, of the general anesthetic actions of ethanol.
Jang
Hye-Ock
HO
Research Institute for Oral Biotechnology, College of Dentistry, Pusan National University, Busan 602-739, Korea.
Huh
Min-Hoi
MH
Lee
Seung-Woo
SW
Lee
Young-Ho
YH
Lee
Jong-Hwa
JH
Seo
Jun-Bong
JB
Koo
Kyo-Il
KI
Jin
Seong-Deok
SD
Jeong
Je-Hyung
JH
Lim
Jang-Seop
JS
Bae
Moon-Kyung
MK
Yun
Il
I
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D002492:Central Nervous System Depressants / Q000494:pharmacology; D004161:Diphenylhexatriene; D000431:Ethanol / Q000494:pharmacology; D020404:Glycerophospholipids / Q000494:pharmacology*; D006825:Hybridomas; D008051:Lipid Bilayers / Q000378:metabolism*; D008081:Liposomes; D008560:Membrane Fluidity / Q000187:drug effects*; D011721:Pyrenes; D013843:Thiazines
C104364:1,2-dimyristoyl-sn-glycero-3-phosphatidylethanol; D002492:Central Nervous System Depressants; D020404:Glycerophospholipids; D008051:Lipid Bilayers; D008081:Liposomes; D011721:Pyrenes; D013843:Thiazines; C053505:fluorescent dye D 282; D004161:Diphenylhexatriene; D000431:Ethanol; C035116:1,3-di-(1-pyrenyl)propane