Biofunctionalization of fluorescent-magnetic-bifunctional nanospheres and their applications.
Hydrazide-containing bifunctional nanospheres were covalently coupled on the surface with IgG, avidin, and biotin, to generate novel fluorescent-magnetic-biotargeting trifunctional nanospheres, which can be used in a number of biomedical applications, including visual sorting and manipulation of apoptotic cells as demonstrated here.
Wang
Guo-Ping
GP
College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, Wuhan University, Wuhan 430072, P. R. China.
Song
Er-Qun
EQ
Xie
Hai-Yan
HY
Zhang
Zhi-Ling
ZL
Tian
Zhi-Quan
ZQ
Zuo
Chao
C
Pang
Dai-Wen
DW
Wu
Dao-Cheng
DC
Shi
Yun-Bo
YB
D000447:Aldehydes / Q000737:chemistry; D017304:Annexin A5; D017209:Apoptosis / Q000528:radiation effects; D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D002853:Chromatography, Liquid; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G / Q000737:chemistry; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D014466:Ultraviolet Rays
D000447:Aldehydes; D017304:Annexin A5; D005456:Fluorescent Dyes; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D001360:Avidin; D001710:Biotin; D016650:Fluorescein-5-isothiocyanate