Quantification of immunoglobulin free light chains in cerebrospinal fluid by nephelometry.
Oligoclonal free light chains (FLC) banding has been described in multiple sclerosis (MS) and should be correlated with disease activity. However, discrepancies between studies have been reported because of differences in methods. A new quantitative, rapid, and automated method using nephelometry is now available. Our objective was to investigate the interest of this method for the diagnosis and prognosis of MS. For this purpose, FLC index was determined in paired samples of CSF and serum from consecutive and unselected patients from the same department of neurology. We enrolled 89 patients (33 MS, 15 "possible MS", and 41 controls) and correlated with IgG index, IgG oligoclonal banding, and clinical MS progression criteria. The main results were (1) FLC kappa index was more sensitive but less specific than IgG index for the diagnosis of MS, (2) two MS patients were negative for oligoclonal banding but exhibited a positive kappa index, (3) no relation between FLC kappa indices, MS clinical criteria, and disease progression was found. In conclusion, FLC kappa index should be considered as a useful complementary test for MS diagnosis. Its pronostic interest remains to be determined on a larger cohort of possible MS patients.
Desplat-Jégo
Sophie
S
Laboratoire d'Immunologie, Hôpital de la Conception, 147, Bd Baille, 13005 Marseille/UMR 6184, Faculté de Médecine Timone, 27, Bd Jean Moulin, 13005 Marseille, France.
Feuillet
Lionel
L
Pelletier
Jean
J
Bernard
Dominique
D
Chérif
André Ali
AA
Boucraut
José
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018450:Disease Progression; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid; D007147:Immunoglobulin Light Chains / Q000097:blood / Q000134:cerebrospinal fluid*; D007145:Immunoglobulin kappa-Chains / Q000097:blood / Q000134:cerebrospinal fluid; D007146:Immunoglobulin lambda-Chains / Q000097:blood / Q000134:cerebrospinal fluid; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D009391:Nephelometry and Turbidimetry / Q000379:methods; D012680:Sensitivity and Specificity
D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains