Effect of processed Scutellaria baicalensis on dextran sulfate sodium-induced colitis in mice.
Scutellaria baicalensis Georgi (Labiatae) has been used in the treatment of inflammatory diseases. Drug processing (Poje) is the process of treating crude drugs by several methods before use. The aim of this study was to determine the effect of processed Scutellaria baicalensis on experimental ulcerative colitis induced by dextran sulfate sodium (DSS). The types of processed Scutellaria baicalensis used in this study were parched Scutellaria baicalensis (PS) and rice wine-baked Scutellaria baicalensis (RWBS). Experimental colitis was induced in mice using a daily treatment of 5% DSS in the drinking water for 7 days. The water extracts of processed Scutellaria baicalensis (1 g/kg) were administered orally once a day for 7 days. The mice were divided in four groups: i) water plus DSS group, ii) crude Scutellaria baicalensis (CS) plus DSS group, iii) PS plus DSS group, and iv) RWBS plus DSS group. RWBS ameliorated all of the inflammatory symptoms, such as body weight loss, rectal bleeding and histological damage, compared to CS. Furthermore, RWBS significantly reduced the mucosal myeloperoxidase activity, and TNF-alpha (tumor necrosis factor-alpha), COX-2 (cyclooxygenase-2), NF-kappaB (nuclear factor-kappa B) and chymase expression more than CS. But these effects were not shown in the PS plus DSS group. Efficacy of Scutellaria baicalensis was increased after rice wine baking, but not after parching. The findings in this study suggest that RWBS may be a useful therapeutic agent for ulcerative colitis.
Choi
Yeon-A
YA
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea.
Kang
Ok-Hwa
OH
Park
Hye-Jung
HJ
Tae
Jin
J
Kim
Dae-Ki
DK
Kang
Chon Sik
CS
Choi
Suck-Chei
SC
Yun
Ki-Jung
KJ
Choi
Suck-Jun
SJ
Nah
Yong-Ho
YH
Kim
Young-Ho
YH
Bae
Ki-Hwan
KH
Lee
Young-Mi
YM
D000818:Animals; D015153:Blotting, Western; D003092:Colitis / Q000139:chemically induced / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate / Q000008:administration & dosage / Q000633:toxicity; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry*; D014867:Water; D014920:Wine
D016328:NF-kappa B; D010936:Plant Extracts; D014867:Water; D016264:Dextran Sulfate; D009195:Peroxidase