title: Gene expression profiling in single cells within tissue.
abstract: We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.
authors.affiliation: Aureon Laboratories, 28 Wells Avenue, Yonkers, New York 10701, USA. paola.capodieci@aureon.com
authors.forename: Paola
authors.initials: P
authors.lastname: Capodieci
authors.forename: Michael
authors.initials: M
authors.lastname: Donovan
authors.forename: Heidi
authors.initials: H
authors.lastname: Buchinsky
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Jeffers
authors.forename: Carlos
authors.initials: C
authors.lastname: Cordon-Cardo
authors.forename: William
authors.initials: W
authors.lastname: Gerald
authors.forename: Jon
authors.initials: J
authors.lastname: Edelson
authors.forename: Shailesh M
authors.initials: SM
authors.lastname: Shenoy
authors.forename: Robert H
authors.initials: RH
authors.lastname: Singer
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D016612:Paraffin Embedding / Q000379:methods*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins