Study of the interaction of hexa-amine cobalt (III) ion with DNA by a resonance light scattering technique and its analytical application.
The effect of hexa-amine cobalt cations on the DNA condensation in aqueous solution was investigated by resonance light scattering (RLS). When the relative concentration of hexa-amine cobalt (III) cations to DNA is in the appropriate range, the cations will induce DNA condensation and aggregation, which results in a strong RLS spectrum characterized by a peak at 290.0 nm. The RLS technique is a powerful tool for monitoring DNA condensation and, under optimal conditions, the enhanced RLS intensity at 290.0 nm was proportional to the concentration of DNA in the range 0.01-6.0 microg/mL. Based on this, a sensitive and convenient analysis method for the microdetermination of DNA was established. The detection limit (3 s) of calf thymus DNA by the proposed method is 1.9 ng/mL and few substances interfere in the DNA determination.
Li
Zheng-ping
ZP
College of Chemistry and Environment Science, Hebei University, Baoding 071002, Hebei Province, People's Republic of China. lzpbd@mail.hbu.edu.cn
Li
Yan-kun
YK
Wang
Yu-cong
YC
D000818:Animals; D002417:Cattle; D003035:Cobalt / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D008027:Light; D008163:Luminescent Measurements; D009691:Nucleic Acid Denaturation; D009994:Osmolar Concentration; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D017186:Titrimetry
D007477:Ions; C030862:cobalt ammonium complex; D003035:Cobalt; D004247:DNA; C427203:calf thymus DNA