title: Simple and effective technical procedures for the packing and application of nanospray PicoFrit HPLC columns for proteomic analyses.
abstract: A simple procedure was developed for packing PicoFrit HPLC columns with chromatographic stationary phase using a reservoir fabricated from standard laboratory HPLC fittings. Packed columns were mounted onto a stainless steel ultra-low volume precolumn filter assembly containing a 0.5-microm pore size steel frit. This format provided a conduit for the application of the nanospray voltage and protected the column from obstruction by sample material. The system was characterised and operational performance assessed by analysis of a range of peptide standards (n = 9).
authors.affiliation: Department of Transmissible Molecular Biology, Veterinary Laboratories Agency, Addlestone, Surrey, UK.
authors.forename: Laurence C
authors.initials: LC
authors.lastname: Howells
authors.forename: Adam O
authors.initials: AO
authors.lastname: Whelan
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Woodward
authors.forename: Nick G
authors.initials: NG
authors.lastname: Coldham
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D040901:Proteomics / Q000295:instrumentation
chemical_list: D010455:Peptides