Effect of oxygen-glucose deprivation on degranulation and histamine release of mast cells.
The purpose of this study was to investigate the effect of oxygen-glucose deprivation (OGD) on degranulation and histamine release of mast cells. Cultured mast cells were exposed to OGD for 1, 2, 4, 8, or 16 h. At 2 h of OGD exposure, the degranulation percentage of mast cells had increased and subsequently showed a progressive further increase, associated with a similar change in lactate dehydrogenase release. Histamine release increased significantly from 1 h of OGD exposure. These results indicate that OGD induces mast cells to degranulate, possibly via a cytotoxic response. This in vitro ischemic model of mast cells might clarify their roles in the pathological processes induced by cerebral ischemia.
Hu
Weiwei
W
Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou, China, 310031.
Shen
Yao
Y
Fu
Qiuli
Q
Dai
Haibin
H
Tu
Hongming
H
Wei
Erqing
E
Luo
Jianhong
J
Chen
Zhong
Z
D000818:Animals; D015550:Cell Degranulation / Q000502:physiology; D015687:Cell Hypoxia / Q000502:physiology; D005947:Glucose / Q000172:deficiency*; D006636:Histamine Release / Q000502:physiology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley
D007770:L-Lactate Dehydrogenase; D005947:Glucose; D010100:Oxygen