[The protective effect of cholic acid on an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells].
OBJECTIVE
To establish an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells of rats and observe the protective effect of cholic acid.


METHODS
Cultured rat microvascular endothelial cells were subjected to the oxygen-glucose deprivation (OGD) (Krebs solution) and recovery of oxygen-glucose, which simulated in vitro ischemia and reperfusion injury, and treated with cholic acid. The A value was measured with MIT chromatometry.


RESULTS
Cultured cells were impaired after OGD for 4 hours and recovery of oxygen-glucose for 12 hours, the A value of the cells treated with cholic acid was significantly higher than that of the cells without treatment (P < 0.01).


CONCLUSIONS
Cholic acid could obviously protect rat cerebral microvascular endothelial cells from injury induced by an in vitro ischemia-reperfusion.
Gao
Yong-Hong
YH
The cerebropathia laboratory of Dongzhinen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China.
Yuan
Zheng-Zhong
ZZ
Niu
Fu-Ling
FL
Cui
Wei
W
Zhu
Ling-Qun
LQ
Wang
Shuo-Ren
SR
D000818:Animals; D001921:Brain / Q000098:blood supply*; D002545:Brain Ischemia / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019826:Cholic Acid / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects / Q000473:pathology*; D008297:Male; D008833:Microcirculation; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology*
D018696:Neuroprotective Agents; D019826:Cholic Acid