Separation of microorganisms using electromigration techniques.
Like other colloidal particles bacteria have a surface charge that originates from the ionization of surface molecules and of the adsorption of ions from solution. Bacterial cell wall and membranes containing numerous proteins, lipid molecules, teichoic acids, lipopolisaccharides which give them characteristic charge. Therefore, bacterial cells undergo electrophoresis in a free solution with their own mobility depending on ionic strength and pH of buffer solution. Various electromigration techniques can be used to separate and determine the intact cells. Successful separation of five species of bacteria was obtained using a trimethylchlorosilane-modified capillary and a divinylbenzene-modified with suppressed EOF over a short distance (8.5 cm). The utilization of coated capillaries prevents adsorption of bacteria to the capillary wall. Another approach is utilization of a dilute dissolved polymer, polyethylene oxide (PEO) in the running buffer as a non-bonded coating for the purpose of altering the EOE These experiment have proved the possibility of diagnosing a variety of diseases and the ability to separate and identify viable cells.
Szumski
Michał
M
Department of Environmental Chemistry and Ecoanalytics, Faculty of Chemistry, Nicolas Copernicus University, ul. Gagarina 7, 87-100 Toruń, Poland.
Kłodzińska
Ewa
E
Buszewski
Bogusław
B
D001419:Bacteria / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D014297:Trimethylsilyl Compounds; D014753:Vinyl Compounds
D014297:Trimethylsilyl Compounds; D014753:Vinyl Compounds; C039293:trimethylchlorosilane; C004985:divinyl benzene