Y chromosome microdeletions: are they implicated in teratozoospermia?
BACKGROUND
Y chromosome microdeletions are known to impair spermatogenesis. Screenings for these microdeletions are performed mostly in patients with sperm count abnormalities.


METHODS
We have screened the Y chromosome of 80 infertile patients with sperm morphological abnormalities. DNA from sperm, peripheral blood or single sperm following multiple displacement amplification (MDA) was utilized to amplify 20 specific sequence-tagged sites (STS) by PCR.


RESULTS
Y chromosome microdeletions were detected in sperm DNA from four of the teratozoospermic patients; while none of the 53 men with normal sperm morphology had any deletions. Two of the four patients with deletions also provided peripheral blood and a fresh semen sample. Both patients had none of the STS deleted in the peripheral blood DNA. Y chromosome microdeletion analysis in the MDA amplified SRY-positive single sperm DNA confirmed the presence of the same deletion in all 10 sperm for one patient and eight out of 10 sperm in the second patient.


CONCLUSIONS
Our observations suggest that some of the teratozoospermia might be related to gonadal mosaic Y chromosome microdeletions. Gonadal mosaicism can be a source of de novo transmissions of Y chromosome microdeletions. The application of MDA can yield enough DNA from a single sperm for genetic analyses.
Hellani
Ali
A
Department of Pathology, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
Al-Hassan
Saad
S
Al-Duraihim
Adel
A
Coskun
Serdar
S
D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000648:ultrastructure*; D004247:DNA / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D017353:Gene Deletion; D020022:Genetic Predisposition to Disease; D016678:Genome; D015894:Genome, Human; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites; D012729:Sex Chromosome Aberrations; D013076:Sperm Count; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology
D017931:DNA Primers; D004247:DNA