Differences in circadian variations of tissue factor pathway inhibitor type 1 between able-bodied and spinal cord injured.
BACKGROUND
Tissue factor pathway inhibitor type 1 (TFPI) is the physiological inhibitor of the tissue factor pathway of coagulation. TFPI is produced by endothelial cells, and most intravascular TFPI is composed of full-length TFPI associated with the endothelium. Circulating TFPI is mainly truncated and lipoprotein-associated, but a small fraction circulates in a free full-length form. Although hormonal state influences the plasma variation of TFPI between individuals, other factors like temporal variation may be important. Hence, in the current study we aimed at exploring the intra-individual variation with focus on the possible circadian variations of TFPI.


METHODS
TFPI free and total antigen from 8 able-bodied and 6 tetraplegic men were measured at 12 time points during a 24 h period.


RESULTS
TFPI free antigen in the able-bodied exhibited circadian variation with the highest levels (approximately 20% above mean) from 12:00 to 18:00 h and the lowest levels (approximately 15% below mean) at 09:00 and 02:00 h. In contrast, TFPI free antigen in the tetraplegic group showed no circadian variation. TFPI total antigen exhibited circadian variation in neither group, but mean TFPI total antigen was lower in the tetraplegic group compared with the able-bodied (80 versus 110 ng/mL, respectively). Notably, even if TFPI total antigen in both groups did not vary according to any specific circadian rhythm, the intra-individual variation was higher than the assay variation.


CONCLUSIONS
TFPI free antigen exhibited circadian variations in able-bodied, but not in tetraplegic subjects and the able-bodied had higher levels of TFPI total antigen than the tetraplegic group.
Dahm
Anders E A
AE
Department of Haematology, Ullevaal University Hospital, Oslo, Norway. a.e.a.dahm@medisin.uio.no
Iversen
Per Ole
PO
Hjeltnes
Nils
N
Sandset
Per Morten
PM
D000328:Adult; D016022:Case-Control Studies; D002940:Circadian Rhythm; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011782:Quadriplegia / Q000097:blood*; D013119:Spinal Cord Injuries / Q000097:blood*
D008074:Lipoproteins; C051928:lipoprotein-associated coagulation inhibitor