[Effects of A23187 on intracellular free Ca2+ concentration and apoptosis on human squamous cell carcinoma of larynx].
OBJECTIVE
To study the effects of A23187 on intracellular free Ca2+ concentration and apoptosis on human squamous cell carcinoma of larynx.


METHODS
Human squamous cell carcinoma of larynx were randomly divided into 4 groups ( 3 treated with A23187 groups and 1 control). Intracellular free Ca2+ was measured by using the fluorescence Ca2+ indicator Fura-2/AM; Apoptotic rate was measured by using FACS 420 Flow Cytometer; DNA gel electrophoresis at different time points.


RESULTS
Intracellular [Ca2+]i at 5 h, 24 h, 48 h after A23187 exposure were (123.48+/-21.54) mmol/L, (110.54+/-11.32) mmol/L, (88.63+/-9.95) mmol/L and all were higher than control group (54.23+/-8.73) nmol/L (P <0.01); Apoptotic rate in 5, 24, 48 h were (3.84+/-1.12), (7.65+/-1.35), (9.49+/-1.31) and also higher than control group (2.63+/-0.75) (P < 0.01) and increased with time prolonged.


CONCLUSIONS
Exposuring to A23187 would interfere with intracellular free Ca2+ concentration and apoptotic rate in human squamous cell carcinoma of larynx suggests that there may be certain relation between them.
Zhang
Xiaowen
X
Department of Otolaryngology-Head and Neck Surgery, the Third Affiliated Hospital of Sun-Yat-Sen University, Guangzhou, 510630, China.
Kong
Weijia
W
D017209:Apoptosis / Q000187:drug effects*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000032:analysis*; D045744:Cell Line, Tumor; D006801:Humans; D007476:Ionophores / Q000494:pharmacology*; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*
D007476:Ionophores; D000001:Calcimycin; D002118:Calcium