Effects of electroacupuncture stimulation applied to limb and back on mesenteric microvascular hemodynamics.
The effects of electroacupuncture stimulation (EAS) of the hind paw and the back on the mesenteric microhemodynamics in anesthetized rats were investigated using an intravital microscope system. Red blood cell (RBC) velocity in the mesenteric arterioles was measured by the dual-sensor method developed by the authors. Electrical stimulation was applied using two acupuncture needles inserted into the skin and the underlying muscles of the hind paw and the dorsal Th13-L1 level area. The hind-paw EAS evoked intensity-dependent pressor responses and increase responses in RBC velocity in mesenteric precapillary arterioles, while the back EAS evoked depressor responses and decrease responses in RBC velocity. Heart rate showed increase responses accompanying EAS either on the hind paw or the back. The pressor responses and increase responses in RBC velocity in mesenteric precapillary arterioles accompanying the hind paw EAS were abolished by an intravenous administration of alpha-adrenergic receptor antagonist (phenoxybenzamine; POB), and the tachycardiac responses were abolished by administration of beta-adrenergic receptor antagonist (propranolol). Occasional but notable reflex vasoconstrictions in the mesenteric terminal arteriole were induced by EAS either on the hind paw or the back. These vasoconstrictive responses were not affected by the administration of POB. The present study directly demonstrated that hemodynamic changes at the level of precapillary arterioles accompanying EAS either on the hind paw or the back mainly depend on the changes of systemic arterial pressure regardless of stimulus current intensities. Moreover, the results in the present study suggest some receptors other than alpha-adrenergic receptor might be involved in the mechanism of EAS-induced vasoconstriction in the mesenteric arteriole.
Takagi
Kentaro
K
Department of Biomedical Engineering, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki 305-8575, Japan.
Yamaguchi
Shinjiro
S
Ito
Momoyo
M
Ohshima
Norio
N
D015669:Acupuncture Points; D000317:Adrenergic alpha-Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D001415:Back; D001783:Blood Flow Velocity / Q000187:drug effects; D015671:Electroacupuncture; D006339:Heart Rate / Q000187:drug effects; D006439:Hemodynamics; D006614:Hindlimb; D007275:Injections, Intravenous; D008297:Male; D008833:Microcirculation / Q000502:physiology; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000294:innervation; D010643:Phenoxybenzamine / Q000008:administration & dosage / Q000494:pharmacology; D011433:Propranolol / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013152:Splanchnic Circulation / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology; D014666:Vasomotor System / Q000502:physiology
D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D010643:Phenoxybenzamine; D011433:Propranolol