title: Spatially controlled co-culture of neurons and glial cells.
abstract: The ability to create and maintain neuron and glial cell co-cultures is important for neuronal regeneration as well as for fundamental studies on neuron and glial cell interactions. We demonstrate here a method for spatially controlling the arrangement of neurons and glial cells. Line patterns of cell resistant, poly(oligoethyleneglycol methacrylate-co-methacrylic acid), was microcontact printed on various substrates to spatially control the attachment of neurons. Neuron-like cells, PC12 and SH-SY5Y cells, were confined within the unprinted line patterns and extended neurites along the line patterns. Subsequent attachment of glial cells was accomplished by converting the originally cell-resistant line patterns of poly(oligoethyleneglycol methacrylate-co-methacrylic acid) to cell adhesive by electrostatic adsorption of cationic poly-lysine, chitosan, or poly(ethyleneimine). This method for creating patterned co-cultures of neuron and glial cells provides a useful tool for investigating neuron-glial cell interactions and has potential applications in the repair or regeneration of nervous systems.
authors.affiliation: Department of Chemical and Material Engineering, University of Cincinnati, Cincinnati, Ohio 45221-0012, USA.
authors.forename: In Hong
authors.initials: IH
authors.lastname: Yang
authors.forename: Carlos C
authors.initials: CC
authors.lastname: Co
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Ho
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D045744:Cell Line, Tumor; D048271:Chitosan; D018920:Coculture Techniques; D006801:Humans; D019344:Lactic Acid; D009457:Neuroglia; D009474:Neurons; D016716:PC12 Cells; D011091:Polyesters; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D011108:Polymers; D011109:Polymethacrylic Acids; D051381:Rats
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D011109:Polymethacrylic Acids; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); D011094:Polyethyleneimine; D048271:Chitosan