Efficacy of directed misting application of a peroxygen disinfectant for environmental decontamination of a veterinary hospital.
OBJECTIVE
To evaluate effectiveness of 4% peroxymonosulfate disinfectant applied as a mist to surfaces in a large animal hospital as measured by recovery of Staphylococcus aureus and Salmonella enterica serovar Typhimurium.


METHODS
Field trial.


METHODS
Polyester transparencies inoculated with bacteria.


METHODS
Polyester transparencies were inoculated with S aureus or S Typhimurium and placed in various locations in the hospital. After mist application of the peroxygen disinfectant, viable bacterial numbers were quantified and compared with growth from control transparencies to assess reduction in bacterial count.


RESULTS
When applied as a mist directed at environmental surfaces contaminated with a geometric mean of 4.03 x 10(7) CFUs of S aureus (95% confidence interval [CI], 3.95 x 10(7) to 4.11 x 10(7)) or 6.17 x 10(6) CFUs of S Typhimurium (95% Cl, 5.55 x 10(6) to 6.86 x 10(6)), 4% peroxymonosulfate reduced the geometric mean number of viable S aureus by 3.04 x 10(7) CFUs (95% Cl, 8.6 x 10(5) to 1.7 x 10(6)) and S Typhimurium by 3.97 x 10(6) CFUs (95% Cl, 8.6 x 10(5) to 3.5 x 10(6)).


CONCLUSIONS
Environmental disinfection with directed mist application of a 4% peroxymonosulfate solution was successful in reducing counts of bacterial CFUs by > 99.9999%. Directed mist application with this peroxygen disinfectant as evaluated in this study appeared to be an effective and efficient means of environmental disinfection in a large animal veterinary hospital and would be less disruptive than more traditional approaches to intensive environmental cleaning and disinfection.
Patterson
Gage
G
Animal Population Health Institute, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Morley
Paul S
PS
Blehm
Kenneth D
KD
Lee
David E
DE
Dunowska
Magda
M
D000336:Aerosols; D000818:Animals; D015169:Colony Count, Microbial / Q000662:veterinary; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D004783:Environmental Microbiology; D006762:Hospitals, Animal / Q000592:standards*; D010545:Peroxides / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development
D000336:Aerosols; D004202:Disinfectants; D010545:Peroxides; C038288:peroxymonosulfate