SPINAL modulated decoupling in high field double- and triple-resonance solid-state NMR experiments on stationary samples.
Continuous wave irradiation has limited bandwidth for heteronuclear 1H decoupling at high fields and for 13C decoupling in 1H/13C/15N triple-resonance experiments. SPINAL-16 modulation is shown to improve the efficiency of 1H and 13C heteronuclear decoupling on single crystals of peptides and on magnetically aligned samples of membrane proteins in bicelles, which is of particular importance because aqueous samples of biomolecules are lossy at high fields, which limits the strengths of the RF fields that can be applied.
Sinha
Neeraj
N
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, 0307 La Jolla, CA 92093-0307, USA.
Grant
Christopher V
CV
Wu
Chin H
CH
De Angelis
Anna A
AA
Howell
Stanley C
SC
Opella
Stanley J
SJ
D001435:Bacteriophages / Q000737:chemistry*; D002247:Carbon Isotopes; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D006860:Hydrogen Bonding; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D010455:Peptides; D005998:Glycine