Depletion of FKBP does not affect the interaction between isolated ryanodine receptors.
The ryanodine receptors/calcium release channels (RyRs) usually form two dimensional regular lattices in the endoplasmic/sarcoplasmic reticulum membranes. The native RyR is associated with many auxiliary proteins, including FKBP. It has been indicated that FKBP may play a role in the intermolecular interaction and coupled gating of neighboring RyRs. However, a more recent study shows that FKBP12 is not involved in the physical linkage between neighboring RyR1s. In the present work, the effect of FKBP12 on the interaction between RyR1s isolated from rabbit skeletal muscle was investigated in an aqueous medium with photon correlation spectroscopy. We found that the depletion of FKBP12 did not affect the oligomerization of RyR1s in the medium containing different [KCl] or under different channel functional states. No evidence is obtained for the involvement of FKBP12 in the intermolecular interaction between RyR1s.
Hu
Xiao-Fang
XF
Bio-X Life Science Research Center, College of Life Science and Biotechnology, Shanghai Jiao Tong University, 1954 Hua-Shan Road, Shanghai 200030, China.
Liang
Xin
X
Chen
Ke-Ying
KY
Zhu
Pei-Hong
PH
Hu
Jun
J
D000818:Animals; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D011485:Protein Binding; D011817:Rabbits; D019837:Ryanodine Receptor Calcium Release Channel / Q000737:chemistry / Q000378:metabolism*; D022061:Tacrolimus Binding Protein 1A / Q000378:metabolism*
D001704:Biopolymers; D019837:Ryanodine Receptor Calcium Release Channel; D022061:Tacrolimus Binding Protein 1A