CXCL16 participates in pathogenesis of immunological liver injury by regulating T lymphocyte infiltration in liver tissue.
OBJECTIVE
To investigate the role of CXCL16 in the pathogenesis of immunological liver injury and to explore the possible mechanism of T lymphocyte infiltration regulated by CXCL16.


METHODS
Immunological liver injury in murine model was induced by Bacille Calmette-Guerin and lipopolysaccharide. Expression pattern and distribution of CXCL16 were examined by real-time quantitative RT-PCR and immunohistochemical analysis. Anti-CXCL16 antibody was administrated in vivo to investigate its effect on T-cell recruitment and acute hepatic necrosis. The survival of murine model was also evaluated.


RESULTS
The murine immunological liver injury model was successfully established. CXCL16 expression increased and predominantly distributed in periportal areas and vascular endothelia in injured liver tissues. Administration of anti-CXCL16 Ab protected the mice from death and acute liver damage. Approximately 70% of the mice survived for 72 h in the anti-CXCL16 Ab treatment group, whereas 80% died within 72 h in control Ab group. The number of liver-infiltrating T lymphocytes was significantly reduced from 1.01 x 10(7) to 3.52 x 10(6)/liver, compared with control Ab treatment.


CONCLUSIONS
CXCL16 is involved in immunological liver injury by regulating T lymphocyte infiltration in liver tissue.
Xu
Huan-Bin
HB
Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, China.
Gong
Yan-Ping
YP
Cheng
Jin
J
Chu
Yi-Wei
YW
Xiong
Si-Dong
SD
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000075743:Chemokine CXCL16; D054427:Chemokine CXCL6; D019743:Chemokines, CXC / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D015870:Gene Expression / Q000276:immunology; D008107:Liver Diseases / Q000276:immunology* / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051116:Receptors, Scavenger; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*
D000906:Antibodies; D000075743:Chemokine CXCL16; D054427:Chemokine CXCL6; D019743:Chemokines, CXC; C498926:Cxcl16 protein, mouse; D008565:Membrane Proteins; D011971:Receptors, Immunologic; D051116:Receptors, Scavenger