title: Effects of pixantrone on immune-cell function in the course of acute rat experimental allergic encephalomyelitis.
abstract: Pixantrone is an immunesuppressor similar to mitoxantrone but with lower cardiotoxicity. We evaluated the effect of pixantrone on B cells and lymphomononuclear cells in the course of acute EAE. Pixantrone reduced the number of B cells and suppressed myelin basic protein (MBP) specific IgG production. In vitro, pixantrone induced apoptosis of rat B lymphocytes in a way similar to mitoxantrone. In addition, pixantrone inhibited antigen specific and mitogen induced lymphomononuclear cell proliferation, as well as IFN-gamma production, during EAE. These findings suggest a similar mechanism of action for pixantrone and mitoxantrone on the effector function of lymphomonocyte B and T cells.
authors.affiliation: Department of Neurological and Psychiatric Sciences, University of Florence, Florence, Italy.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Mazzanti
authors.forename: Tiziana
authors.initials: T
authors.lastname: Biagioli
authors.forename: Alessandra
authors.initials: A
authors.lastname: Aldinucci
authors.forename: Guido
authors.initials: G
authors.lastname: Cavaletti
authors.forename: Ennio
authors.initials: E
authors.lastname: Cavalletti
authors.forename: Norberto
authors.initials: N
authors.lastname: Oggioni
authors.forename: Maura
authors.initials: M
authors.lastname: Frigo
authors.forename: Stefania
authors.initials: S
authors.lastname: Rota
authors.forename: Elena
authors.initials: E
authors.lastname: Tagliabue
authors.forename: Clara
authors.initials: C
authors.lastname: Ballerini
authors.forename: Luca
authors.initials: L
authors.lastname: Massacesi
authors.forename: Paolo
authors.initials: P
authors.lastname: Riccio
authors.forename: Francesco
authors.initials: F
authors.lastname: Lolli
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000906:Antibodies / Q000097:blood; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D048749:Cytokinesis; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D007546:Isoquinolines / Q000627:therapeutic use*; D004676:Myelin Basic Protein / Q000008:administration & dosage / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000187:drug effects; D013997:Time Factors
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D016207:Cytokines; D007166:Immunosuppressive Agents; D007546:Isoquinolines; D004676:Myelin Basic Protein; C086548:pixantrone