title: Syndecan recycling [corrected] is controlled by syntenin-PIP2 interaction and Arf6.
abstract: Syndecans are heparan sulfate proteoglycans that modulate the activity of several growth factors and cell adhesion molecules. PDZ domains in the adaptor protein syntenin interact with syndecans and with the phosphoinositide PIP(2), which is involved in the regulation of the actin cytoskeleton and membrane trafficking. Here, we show that the syntenin PDZ domain-PIP(2) interaction controls Arf6-mediated syndecan recycling through endosomal compartments. FGF receptor accompanies syndecan along the syntenin-mediated recycling pathway, in a heparan sulfate- and FGF-dependent manner. Syndecans that cannot recycle via this pathway become trapped intracellularly and inhibit cell spreading. This syntenin-mediated syndecan recycling pathway may regulate the surface availability of a number of cell adhesion and signaling molecules.
authors.affiliation: Laboratory for Glycobiology and Developmental Genetics, Department of Human Genetics, University of Leuven, Belgium. pascale.zimmerman@med.kuleuven.be
authors.forename: Pascale
authors.initials: P
authors.lastname: Zimmermann
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Gisèle
authors.initials: G
authors.lastname: Degeest
authors.forename: Eva
authors.initials: E
authors.lastname: Mortier
authors.forename: Iris
authors.initials: I
authors.lastname: Leenaerts
authors.forename: Christien
authors.initials: C
authors.lastname: Coomans
authors.forename: Joachim
authors.initials: J
authors.lastname: Schulz
authors.forename: Francisca
authors.initials: F
authors.lastname: N'Kuli
authors.forename: Pierre J
authors.initials: PJ
authors.lastname: Courtoy
authors.forename: Guido
authors.initials: G
authors.lastname: David
mesh_terms: D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D011992:Endosomes / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008954:Models, Biological; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000737:chemistry / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D053669:Syndecan-2; D053667:Syndecans; D053676:Syntenins
chemical_list: D000089982:ADP-Ribosylation Factor 6; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D019269:Phosphatidylinositol 4,5-Diphosphate; D011509:Proteoglycans; C508245:SDC2 protein, human; C109621:SDCBP protein, human; C419574:SDCBP2 protein, human; D053667:Syndecans; D053676:Syntenins; D053669:Syndecan-2; D020727:ADP-Ribosylation Factors; C000715348:ARF6 protein, human