[Mechanisms of heat shock proteins inhibiting C2C12 cell apoptosis induced by hydrogen peroxide].
OBJECTIVE
To explore the mechanisms of C2C12 cell apoptosis induced by hydrogen peroxide (H2O2) which is inhibited by heat shock proteins.


METHODS
The expression of heat shock proteins in mouse embryonic myogenic cell line, C2C12 cell,was induced by heat shock response. C2C12 cell apoptosis induced by 0.5 mmol/L hydrogen peroxide (H2O2) was determined by Hoechst 33258 staining. The activities of caspase -3,8,9 were assayed by caspase colorimetric assay kit and Western-blotting. The release of cytochrome C from mitochondria was observed by Western-blotting of cell mitochondria and cytosol fractions.


RESULTS
The expression of HSP70 and alphaB-crystallin in C2C12 cell significantly increased at 24 hour after the heat shock response. Heat shock response could inhibit the release of cytochrome c from mitochondria to cytoplasm,the activation of caspase -3,8,9 and the subsequent apoptosis induced by H2O2 in C2C12 cell.


CONCLUSIONS
HSPs can inhibit the C2C12 cell apoptosis through interference with the activation of both mitochondrial and death receptor pathways, which can provide new clues for the prevention of cardiovascular diseases.
Xiao
Wei-Min
WM
Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Jiang
Bi-Mei
BM
Shi
Yong-Zhong
YZ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D045304:Cytochromes c / Q000378:metabolism; D004622:Embryo, Mammalian; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D051379:Mice; D009206:Myocardium / Q000473:pathology*
D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D045304:Cytochromes c; D006861:Hydrogen Peroxide; C505409:Casp3 protein, mouse; C505526:Casp8 protein, mouse; C506434:Casp9 protein, mouse; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases