[The influence of platelets on PTA1 expression and PDGF release from ECV304 cells].
OBJECTIVE
To study the influence of platelets on the expression of platelet/T cell activation antegen 1 (PTA1) and release of platelet-derived growth factor (PDGF) from vascular endothelial cells (ECV304) stimulated with pregnancy induced hypertention (PIH) patient sera.


METHODS
The expression of PTA1 on ECV304 cells stimulated with PIH patient sera and incubated with or without platelets was analyzed by flow cytometry. ELISA was employed to measure the quantity of PDGF released from ECV304 cells.


RESULTS
After incubation with platelets, PTA1 expression on ECV304 cells stimulated with PIH patient sera decreased. PIH sera induced more PDGF release from ECV304 cells than sera from normal pregnant women. Coculture with platelets increased PDGF release from ECV304 stimulated with PIH patient sera.


CONCLUSIONS
PTA1 molecule may mediate the adhesion between ECV304 cells and platelets, which lead to the release of PDGF.
Li
Chang-ling
CL
Department of Laboratory Diagnosis, Punan Hospital, Shanghai 200125, China. Lchl12@yahoo.com
Xu
Rui-huan
RH
Zhang
Hong-de
HD
Yin
Xue-nian
XN
D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D001792:Blood Platelets / Q000502:physiology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000097:blood* / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D011247:Pregnancy; D055815:Young Adult; D000098862:T Lineage-Specific Activation Antigen 1
D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; D010982:Platelet-Derived Growth Factor