Comparison of different methods for radiochemical purity testing of [99mTc-EDDA-HYNIC-D-Phe1,Tyr3]-octreotide.
[99mTc-EDDA-HYNIC-D-Phe1,Tyr3]-octreotide (99mTc-EDDA-HYNIC-TOC) is an alternative radioligand for somatostatin receptor (SSTR) scintigraphy of neuroendocrine tumours. In order to allow a rapid and accurate determination of the quality in the clinical routine the aim of this study was to evaluate different methods of radiochemical purity (RCP) testing. Three different methods of RCP testing were compared: high-performance liquid chromatography (HPLC), thin layer chromatography (TLC) and minicolumn (Sep-Pak purification = SPE). HPLC was shown to be the most effective method for the quality control. The use of TLC and SPE is only recommended after sufficient practical labelling experience.
von Guggenberg
Elisabeth
E
Clincal Department of Nuclear Medicine, Innsbruck Medical University, Anichstrasse 35, 6020 Innsbruck, Austria. elisabeth.vonguggenberg@uklibk.ac.at
Penz
Barbara
B
Kemmler
Georg
G
Virgolini
Irene
I
Decristoforo
Clemens
C
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006801:Humans; D016014:Linear Models; D015282:Octreotide / Q000031:analogs & derivatives* / Q000032:analysis; D019275:Radiopharmaceuticals / Q000032:analysis*; D012822:Silicon Dioxide
D019275:Radiopharmaceuticals; D012822:Silicon Dioxide; C042953:octadecyl silane-bonded silica; D015282:Octreotide