[Expression of SOCS-1, 3 induced by Chlamydia trachomatis-infected Hela229].
OBJECTIVE
To explore the relationship between SOCS, immune suppression and chlamydial persistence of Chlamydia trachomatis-infected cells.


METHODS
After being infected with Chlamydia trachomatis, RT-PCR was used to examine the expression of SOCS-1, 3 mRNA and Western blotting to examine the expression of SOCS-1,3 protein in Hela229 cells. Immunofluorescence was used to examine the effect of SOCS obstruction on the multiplication of Chlamydia trachomatis.


RESULTS
Chlamydia trachomatis-infected Hela229 cells can induce the expression of SOCS-1,3 both on mRNA level and on protein level. The multiplicity of Chlamydia trchomatis can be suppressed after the expression of SOCS-1,3 was obstructed.


CONCLUSIONS
Chlamydia trachomatis-infected Hela229 cells can induce the expression of SOCS-1,3, which is one of the mechanisms that Chlamydia trachomatis can suppress host immune and result in persistence.
Wei
Xiu-qing
XQ
Department of Immunology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Cheng
Wen
W
Yan
Jie
J
Mei
Bing
B
Huo
Zhi
Z
Yu
Ping
P
D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000254:growth & development / Q000472:pathogenicity*; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D007166:Immunosuppressive Agents; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis* / Q000235:genetics
D007166:Immunosuppressive Agents; D012333:RNA, Messenger; C106644:SOCS3 protein, human; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins