title: Comparison of extracellular matrix and apoptotic markers between benign lesions and carcinomas in human breast.
abstract: The expression of the extracellular matrix-related genes, such as fibronectin, laminin and tenascin C, and apoptosis-related genes, such as bax, bcl2 and survivin, was evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and by immunohistochemistry in normal breast tissue and benign and malignant breast tumors and then correlated to several clinical parameters: estrogen and progesterone receptors, Ki67, ErbB2, tumor size, lymph node status and grading. Seventy-three breast tissue samples were examined. After RNA extraction, an RT-PCR was performed to detect fibronectin, laminin, tenascin C, bax, bcl2 and survivin gene expression. Thirty-two samples were evaluated also by immunohistochemistry at the protein level to detect fibronectin, laminin, tenascin C, bax and survivin. We found a significant correlation (P=0.025) between fibronectin gene expression and lymph node status, and a significant negative correlation (P=0.049) between laminin gene expression and Ki67. In addition, we found a statistically significant increase in survivin transcription in malign tumors compared to fibroadenomas (P=0.024). The negative correlation between laminin transcription and Ki67 could suggest that laminin impacts negatively on tumor proliferation, and the positive correlation between fibronectin and lymph node status may lead to consider fibronectin as predictive of long distance metastasis.
authors.affiliation: Department of Experimental Medicine and Pathology, University of Rome La Sapienza, Rome, Italy.
authors.forename: Fortunata
authors.initials: F
authors.lastname: Vasaturo
authors.forename: Elvira
authors.initials: E
authors.lastname: Sallusti
authors.forename: Angela
authors.initials: A
authors.lastname: Gradilone
authors.forename: Carolina
authors.initials: C
authors.lastname: Malacrino
authors.forename: Tiziana
authors.initials: T
authors.lastname: Nardo
authors.forename: Grazia
authors.initials: G
authors.lastname: Avagnano
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Aglianò
authors.forename: Teresa
authors.initials: T
authors.lastname: Granato
authors.forename: Bruno
authors.initials: B
authors.lastname: De Vincenzi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Coppotelli
authors.forename: Antonella
authors.initials: A
authors.lastname: Marzullo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Soda
authors.forename: Lucilla
authors.initials: L
authors.lastname: Simonelli
authors.forename: Mauro
authors.initials: M
authors.lastname: Modesti
authors.forename: Susanna
authors.initials: S
authors.lastname: Scarpa
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor; D001941:Breast Diseases / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003593:Cytoplasm / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins; D019394:Ki-67 Antigen / Q000096:biosynthesis; D007797:Laminin / Q000378:metabolism; D008207:Lymphatic Metastasis; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D019063:Tenascin / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D005353:Fibronectins; D051034:Inhibitor of Apoptosis Proteins; D019394:Ki-67 Antigen; D007797:Laminin; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012313:RNA; D018719:Erb-b2 Receptor Tyrosine Kinases; D000077022:Survivin; D019063:Tenascin; D051028:bcl-2-Associated X Protein; C107489:BIRC5 protein, human