Response to gamma-irradiation in V79 cells conditioned by repeated treatment with low doses of hydrogen peroxide.
Chinese hamster V79 cells were conditioned by repeated treatment with low doses of hydrogen peroxide. After this treatment, the conditioned cells were compared to parental V79 cells with regard to different endpoints. It was found that, compared to parental cells, the conditioned cells tolerated low serum concentrations better, they suffered from higher levels of aneuploidy, and they showed enhanced antioxidant defense. When exposed to gamma-rays, they suffered from lipid peroxidation to a lesser extent, were more resistant to cell killing, exhibited higher mutation frequency at the HGPRT locus, and showed lower frequency of apoptosis. These cells also induced antioxidant enzymes in response to gamma-ray exposure that differently was from than the parental cells. Overall, the data suggest a stable adaptive response in the conditioned cells.
Bose Girigoswami
Koyeli
K
Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, 741235 Nadia, India.
Ghosh
Rita
R
D000222:Adaptation, Physiological; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D055592:Biophysical Phenomena; D001703:Biophysics; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D005720:Gamma Rays / Q000009:adverse effects*; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D009154:Mutation; D016877:Oxidants / Q000008:administration & dosage / Q000633:toxicity
D000975:Antioxidants; D016877:Oxidants; D006861:Hydrogen Peroxide