title: ST6GalNAc I expression in MDA-MB-231 breast cancer cells greatly modifies their O-glycosylation pattern and enhances their tumourigenicity.
abstract: Sialyl-Tn is a carbohydrate antigen overexpressed in several epithelial cancers, including breast cancer, and usually associated with poor prognosis. Sialyl-Tn is synthesized by a CMP-Neu5Ac:GalNAcalpha2,6-sialyltransferase: CMP-Neu5Ac: R-GalNAcalpha1-O-Ser/Thr alpha2,6-sialyltransferase (EC 2.4.99.3) (ST6GalNAc I), which transfers a sialic acid residue in alpha2,6-linkage to the GalNAcalpha1-O-Ser/Thr structure. However, established breast cancer cell lines express neither ST6GalNAc I nor sialyl-Tn. We have previously shown that stable transfection of MDA-MB-231, a human breast cancer cell line, with ST6GalNAc I cDNA induces sialyl-Tn antigen (STn) expression. We report here the modifications of the O-glycosylation pattern of a MUC1-related recombinant protein secreted by MDA-MB-231 sialyl-Tn positive cells. We also show that sialyl-Tn expression and concomitant changes in the overall O-glycan profiles induce a decrease of adhesion and an increase of migration of MDA-MB-231. Moreover, STn positive clones exhibit an increased tumour growth in severe combined immunodeficiency (SCID) mice. These observations suggest that modification of the O-glycosylation pattern induced by ST6GalNAc I expression are sufficient to enhance the tumourigenicity of MDA-MB-231 breast cancer cells.
authors.affiliation: Centre Commun de Mesures Imagerie Cellulaire, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d'Ascq, France.
authors.forename: S
authors.initials: S
authors.lastname: Julien
authors.forename: E
authors.initials: E
authors.lastname: Adriaenssens
authors.forename: K
authors.initials: K
authors.lastname: Ottenberg
authors.forename: A
authors.initials: A
authors.lastname: Furlan
authors.forename: G
authors.initials: G
authors.lastname: Courtand
authors.forename: A-S
authors.initials: AS
authors.lastname: Vercoutter-Edouart
authors.forename: F-G
authors.initials: FG
authors.lastname: Hanisch
authors.forename: P
authors.initials: P
authors.lastname: Delannoy
authors.forename: X
authors.initials: X
authors.lastname: Le Bourhis
mesh_terms: D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006031:Glycosylation; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D046188:Protein Modification, Translational; D012799:Sialyltransferases / Q000096:biosynthesis*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; C055491:sialosyl-Tn antigen; D012799:Sialyltransferases; C038525:CMP-N-acetylneuraminate-alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase