Yeast fluorescence microscopy.
Fluorescence microscopy is the essential technique for investigation of the intracellular distribution of macromolecules and various organelles also in yeast cells. In this chapter, detailed practical procedures for fluorescence microscopic observations developed or adopted in our laboratory are described. These include labeling of the cell wall and chitin, F-actin structures, nuclear and mitochondrial DNA, and two different procedures for investigation of yeast cells by immunofluorescence. In addition, our experience with multicolor labeling experiments is introduced and discussed.
Hasek
Jirí
J
Laboratory of Cell Production, Institute of Microbiology, Academy of the Sciences of the Czech Republic.
D000199:Actins / Q000378:metabolism; D002473:Cell Wall / Q000648:ultrastructure; D002686:Chitin / Q000378:metabolism; D004271:DNA, Fungal / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D009172:Mycology / Q000379:methods; D015388:Organelles / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*
D000199:Actins; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D011993:Recombinant Fusion Proteins; D002686:Chitin; D049452:Green Fluorescent Proteins