[Study on the extraction process for cannabinoids in hemp seed oil by orthogonal design].
OBJECTIVE
To select the optimum extracting procedure for cannabinoids from hemp seed oil.


METHODS
The optimum extracting procedure was selected with the content of cannabinol and delta9-tetrehydrocannabinol from hemp seed oil by orthogonal test design. We have examined three factors that may influence the extraction rate: the time of extraction, the times of extraction and the amount of methanol.


RESULTS
The optimum extraction condition was adding 5 ml, two times amount of methanol into hemp seed oil for 15 min.


CONCLUSIONS
The above extraction process gave the most rational, stable, feasible and satisfactory results. The method is convenient.
Zhang
Gang
G
North-east University, Shenyang.
Guo
Jiangning
J
Bi
Kaishun
K
D002186:Cannabinoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002188:Cannabis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D013759:Dronabinol / Q000032:analysis / Q000302:isolation & purification; D000432:Methanol; D010938:Plant Oils / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D014465:Ultrasonics
D002186:Cannabinoids; D010938:Plant Oils; D013759:Dronabinol; D000432:Methanol