Inhibitory effect of Epimedium extract on S-adenosyl-L-homocysteine hydrolase and biomethylation.
In the present paper, the inhibitory effect of Epimedium extract on the activity of S-adenosyl-L-homocysteine (AdoHcy) Hydrolase was studied. The results showed that Epimedium extract inhibited the activity of recombinant human AdoHcy hydrolase in a dose-dependent manner. This inhibitory effect was also observed in hepatic cell line 7701 and hepatoma HepG2, however, the effect in 7701 cells was more potent than in HepG2 cells. The extract could significantly reduce AdoMet/AdoHcy ratio in 7701 cells in a dose-dependent manner, suggesting reduced biomethylation level in 7701 cells. In contrast, it resulted in elevated AdoMet/AdoHcy ratio in the HepG2 cells. The result of MALDI-MS assay indicated that epimedin A and ikarisoside F from the extract could bind to AdoHcy hydrolase. The present data suggested that Epimedium extract could inhibit the activity of AdoHcy hydrolase, thus regulating the cellular biomethylation as well as reducing cellular Hcy level. These results will provide new clues to the mechanisms of Epimedium in curing of cardiovascular disease and regulating tumor cell growth.
Zhang
Xiaoyu
X
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Changchun 130022, China.
Li
Yanjie
Y
Yang
Xiaoda
X
Wang
Kui
K
Ni
Jiazuan
J
Qu
Xiaogang
X
D043383:Adenosylhomocysteinase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000465:Algorithms; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000494:pharmacology; D034181:Epimedium / Q000737:chemistry*; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008745:Methylation; D010936:Plant Extracts / Q000494:pharmacology; D011994:Recombinant Proteins; D012436:S-Adenosylmethionine / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004791:Enzyme Inhibitors; D047309:Flavones; D010936:Plant Extracts; D011994:Recombinant Proteins; D012436:S-Adenosylmethionine; D043383:Adenosylhomocysteinase; D005978:Glutathione