title: Adiponectin is synthesized and secreted by human and murine cardiomyocytes.
abstract: Adiponectin is thought to play a decisive role in the relationships among obesity, insulin resistance and cardiovascular risk. This study investigated whether cardiomyocytes synthesize and secrete adiponectin, and the effects of this hormone on cardiac cells. RT-PCR showed that mouse, rat and human cardiomyocytes produced mRNA for adiponectin and adiponectin receptors 1 and 2. Immunohistochemistry confirmed the presence of adiponectin in the cytoplasm of cultured cardiomyocytes, and radioimmunoassay showed that these cells secreted adiponectin into the culture medium. Exogenous adiponectin enhanced glucose and fatty acid uptake and induced AMPK phosphorylation in cultured cardiomyocytes. Our results demonstrate that adiponectin is synthesized and secreted by isolated murine and human cardiomyocytes, and suggest that the local production of this hormone by cardiomyocytes could be involved in the regulation of cardiac metabolism and function.
authors.affiliation: Unidad de Investigación del Servicio de Cardiología, Hospital Clínico Universitario, Santiago de Compostela, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Piñeiro
authors.forename: María J
authors.initials: MJ
authors.lastname: Iglesias
authors.forename: Rosalía
authors.initials: R
authors.lastname: Gallego
authors.forename: Kawtar
authors.initials: K
authors.lastname: Raghay
authors.forename: Sonia
authors.initials: S
authors.lastname: Eiras
authors.forename: José
authors.initials: J
authors.lastname: Rubio
authors.forename: Carlos
authors.initials: C
authors.lastname: Diéguez
authors.forename: Oreste
authors.initials: O
authors.lastname: Gualillo
authors.forename: José R
authors.initials: JR
authors.lastname: González-Juanatey
authors.forename: Francisca
authors.initials: F
authors.lastname: Lago
mesh_terms: D055372:AMP-Activated Protein Kinases; D052242:Adiponectin; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D005227:Fatty Acids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009097:Multienzyme Complexes / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D052242:Adiponectin; D005227:Fatty Acids; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases; D005947:Glucose