Gender-related differences in recovery of locomotor function after spinal cord injury in mice.
METHODS
In order to study the role of gender in recovery, we induced a thoracic compression spinal cord injury (SCI) separately in 2-month-old male and female C57Bl/6 mice.


OBJECTIVE
We intended to assess effects of gender on recovery of hindlimb motor function and to correlate these with histomorphologic profiles of injured spinal cord tissue.


METHODS
Locomotor function was evaluated by three means: a modified locomotor scoring system for rodents, beam walking and computerized activity meter. Histology was analyzed by comparison of hematoxylin and eosin-stained perfused specimens.


RESULTS
Locomotor scores were 2.2+/-0.9 on day 1 in male mice, while, in contrast, they were significantly higher, 7.3+/-1.7, in females (P<0.02). On day 14 Basso, Beattie and Bresnahan scores were 9.5+/-2.2 in male mice and 16.0+/-2.2 in females (P<0.03). Terminal histology showed that the spinal cord architecture was relatively better preserved in female mice and that the extent of necrosis and infiltration of inflammatory cells was less compared to males.


METHODS
Neurobiology Research Laboratory of University of Kansas Medical School in US Department of Veterans Affairs Medical Center, Kansas City, Missouri.


CONCLUSIONS
We found that the severity of the initial injury as well as the ultimate recovery of motor function after SCI is significantly influenced by gender, being remarkably better in females. The mechanism(s) of neuroprotection in females, although not yet elucidated, may be associated with the effects of estrogen on pathophysiological processes (blood flow, leukocyte migration inhibition, antioxidant properties, and inhibition of apoptosis).


BACKGROUND
Medical Research, US Department of Veterans Affairs, the Christopher Reeve Paralysis Foundation and NIH.
Farooque
M
M
Neurobiology Research Laboratory, Department of Veterans Affairs Medical Center, Kansas City, MO 64128, USA.
Suo
Z
Z
Arnold
P M
PM
Wulser
M J
MJ
Chou
C-T
CT
Vancura
R W
RW
Fowler
S
S
Festoff
B W
BW
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006614:Hindlimb / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000502:physiology*; D009460:Neurologic Examination; D011597:Psychomotor Performance / Q000502:physiology; D020127:Recovery of Function / Q000502:physiology*; D012720:Severity of Illness Index; D012727:Sex Characteristics; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors