[Anticonvulsant effect of phencynonate hydrochloride on maximal electroshock seizure and the metrazol seizure threshold test in mice].
OBJECTIVE
To test the antiepileptic effect of phencynonate hydrochloride and investigate its antiepileptic mechanism.


METHODS
Through establishment of different epilepsy models, antiepileptic effects of phencynonate hydrochloride and other drugs were examined. Besides, the effect of phencynonate hydrochloride and other compounds against NMDA-induced lethality in mice, NMDA-induced injury in rat primary hippocampal neuronal cultures and NMDA-induced current were also observed.


RESULTS
Phencynonate hydrochloride produced a significant anticonvulsant effect on different epilepsy models. Furthermore, phencynonate hydrochloride also exerted its obvious protection against the lethal effects of NMDA in mice, antagonized the NMDA-induced injury in rat primary hippocampal neuronal cultures and blocked NMDA-induced current in a dose-dependent manner.


CONCLUSIONS
Phencynonate hydrochloride had a notable anticonvulsant effect on typical epilepsy models, its antiepileptic mechanism might relate to its antagonism against NMDA receptor.
Wang
Yong-An
YA
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. yonganw908@yahoo.com.cn
Zhou
Wen-Xia
WX
Liu
Yan-Qin
YQ
Zheng
Jian-Quan
JQ
Liu
Ke-Liang
KL
Ruan
Jin-Xiu
JX
D000818:Animals; D000831:Animals, Newborn; D000927:Anticonvulsants / Q000494:pharmacology / Q000627:therapeutic use*; D001372:Aza Compounds / Q000494:pharmacology / Q000627:therapeutic use*; D002478:Cells, Cultured; D004597:Electroshock; D005260:Female; D006016:Glycolates / Q000494:pharmacology / Q000627:therapeutic use*; D006624:Hippocampus / Q000166:cytology*; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D016202:N-Methylaspartate / Q000633:toxicity; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010433:Pentylenetetrazole; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy*
C504860:3-methyl-3-azabicyclo(3,3,1)nonanyl-9-alpha-yl-alpha-cyclopentyl-alpha-phenyl-alpha-glycolate; D000927:Anticonvulsants; D001372:Aza Compounds; D006016:Glycolates; D018696:Neuroprotective Agents; D016202:N-Methylaspartate; D010433:Pentylenetetrazole