title: Lack of molecular relationships between lipid peroxidation and mitochondrial DNA single strand breaks in isolated rat hepatocytes and mitochondria.
abstract: We investigated the molecular relationships between lipid peroxidation and mitochondrial DNA (mtDNA) single strand breaks (ssb) in isolated rat hepatocytes and mitochondria exposed to tert-butylhydroperoxide (TBH). Our results show that mtDNA ssb induced by TBH are independent of lipid peroxidation and dependent on the presence of iron and of hydroxyl free radicals. These data contribute to the definition of the mechanisms whereby mtDNA ssb are induced and provide possible molecular targets for the prevention of this kind of damage in vivo.
authors.affiliation: Dipartimento di Medicina Sperimentale, Sezione di Patologia Generale, Università di Pavia, Piazza Botta 10, 27100 Pavia, Italy. claudia@manuzio.unipv.it
authors.forename: Claudia
authors.initials: C
authors.lastname: Scotti
authors.forename: Luisa
authors.initials: L
authors.lastname: Iamele
authors.forename: Andrea
authors.initials: A
authors.lastname: Alessandrini
authors.forename: Vanio
authors.initials: V
authors.lastname: Vannini
authors.forename: Ornella
authors.initials: O
authors.lastname: Cazzalini
authors.forename: Maria C
authors.initials: MC
authors.lastname: Lazzè
authors.forename: Raffaele
authors.initials: R
authors.lastname: Melli
authors.forename: Monica
authors.initials: M
authors.lastname: Savio
authors.forename: Roberto
authors.initials: R
authors.lastname: Pizzala
authors.forename: Lucia A
authors.initials: LA
authors.lastname: Stivala
authors.forename: Silvia
authors.initials: S
authors.lastname: Biglieri
authors.forename: Aldo
authors.initials: A
authors.lastname: Tomasi
authors.forename: Livia
authors.initials: L
authors.lastname: Bianchi