Biological effects of extracts obtained from Stryphnodendron adstringens on Herpetomonas samuelpessoai.
We report the effect of Stryphnodendron adstringens on the trypanosomatid Herpetomonas samuelpessoai. The parasites were grown at 28 degrees C in a chemically defined medium containing crude extract and fractions at concentrations from 100 to 5000 microg/ml obtained from S. adstringens. Concentrations of 500, 1000, 2500, and 5000 microg/ml both crude extract and semi-purified fraction progressively inhibited the protozoans' growth. At a concentration of 100 microg/ml, crude extract or a semi-purified (F3) fraction did not affect the growth of the protozoans. The F3-9 - F3-12 sub-fractions, at a concentration of 1000 microg/ml, also showed increased inhibitory activity on H. samuelpessoai. The IC50 of the crude extract and the F3 fraction were 538 and 634 microg/ml, respectively. Ultrastructural and enzymatic alterations in the trypanosomatids were also evaluated. H. samuelpessoai cultivated in the presence of IC50 crude extract showed considerable ultrastructural alterations, such as marked mitochondrial swelling with a large number of cristae and evident Golgi complex vesiculation, as observed by transmission electron microscopy. Cells exposed to 538 microg/ml of crude extract at 28 degrees C for 72 h, showed decreased activity of the enzyme succinate cytochrome c reductase, a typical mitochondrion marker, as compared to untreated cells.
Holetz
Fabíola Barbieri
FB
Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, 87020-900 Maringá, PR, Brazil.
Ueda-Nakamura
Tânia
T
Dias Filho
Benedito Prado
BP
Mello
João Carlos Palazzo de
JC
Morgado-Díaz
José Andrés
JA
Toledo
Cleyton Eduardo Mendes de
CE
Nakamura
Celso Vataru
CV
D000818:Animals; D007887:Fabaceae / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000494:pharmacology; D013997:Time Factors; D014351:Trypanosomatina / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure
D010936:Plant Extracts