Anesthetic-induced alteration in expression of myocardial calcium cycling proteins.
OBJECTIVE
The purpose of this study was to test the hypothesis that anesthesia alone or in combination with high F(I)(O)2 alters expression of the myocardial calcium cycling proteins, sarcoplasmic endoreticular calcium adenosine triphosphatase subtype 2a (SERCA2a), and the sarcolemmal sodium-calcium exchanger (NCX).


METHODS
Multigroup comparison of protein expression using analysis of variance.


METHODS
University research laboratory.


METHODS
Twenty-seven New Zealand white rabbits.


METHODS
After sedation and the induction of anesthesia, animals underwent either tracheal intubation and ventilation for 5 hours with 1.0% end-tidal halothane in oxygen (HAL-O(2) , n = 5) or air (HAL-air, n = 5) or time-control recovery while spontaneously breathing oxygen (TC-O(2) , n = 5) or air (TC-air, n = 5) for 5 hours. Halothane dose was based on pilot data from 2 rabbits. Animals were then sacrificed, and the hearts were removed for Western blot analysis. Data were normalized to those from a group of rabbits immediately sacrificed (n = 5) without any prior treatment.


RESULTS
In comparison to their respective time controls, SERCA2a was decreased 23 % in both HAL-air and HAL-O(2) groups, whereas NCX was increased 34% and 122%, respectively. Expression was distinctly different between HAL-air and HAL-O(2) for both SERCA2a (p = 0.009) and NCX (p < 0.001), indicating an influence of high F(I)O(2). Similarly, SERCA2a in the TC-O(2) group was reduced 25% relative to the TC-air group.


CONCLUSIONS
Halothane alters the expression of myocardial calcium cycling proteins, and this effect is potentiated by high F(I)O(2) . These data offer the broad conclusion that perioperative interventions may influence the study of myocardial molecular remodeling and suggest the possibility of anesthetic-induced myocardial molecular remodeling.
Heerdt
Paul M
PM
Department of Anesthesiology, Weill Medical College of Cornell University, Memorial Sloan-Kettering Cancer Center, New York, New York, USA. pmheerd@mail.med.cornell.edu
The
Andrew H S
AH
Markov
Nickolay P
NP
D000704:Analysis of Variance; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001784:Blood Gas Analysis / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D002135:Calcium-Binding Proteins / Q000187:drug effects / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000187:drug effects / Q000378:metabolism*; D006221:Halothane / Q000494:pharmacology*; D007442:Intubation, Intratracheal / Q000379:methods; D009206:Myocardium / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D011817:Rabbits; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D019831:Sodium-Calcium Exchanger / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D018685:Anesthetics, Inhalation; D002135:Calcium-Binding Proteins; D019831:Sodium-Calcium Exchanger; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases; D010100:Oxygen; D006221:Halothane