title: In situ visualization of DSBs to assess the extranuclear/extracellular effects induced by low-dose alpha-particle irradiation.
abstract: Extranuclear/extracellular effects may have a significant effect on low-dose radiation risk assessment as well as on the shape of the dose-response relationship. Numerous studies using different end points such as sister chromatid exchanges, micronuclei and mutation have shown that this phenomenon exists in many cell types. However, these end points mostly reflect the late events after radiation damage, and little is known about the early response in this phenomenon. DNA double-strand breaks (DSBs) induced by ionizing radiation or carcinogenic chemicals can be visualized in situ using gamma-H2AX immunofluorescence staining, and there is evidence that the number of gamma-H2AX foci can be closely correlated with DSBs induced. Here we used gamma-H2AX as a biomarker to assess the extranuclear/extracellular effects induced by low-dose alpha particles in situ. The results show that a greater fraction of positive cells with DSBs (48.6%) was observed than the number of cells whose nuclei were actually traversed by the 1-cGy dose of alpha particles (9.2%). The fraction of DSB-positive cells was greatly reduced after treatment with either lindane or DMSO. These results suggest that in situ visualization of DSBs can be used to assess radiation-induced extranuclear/extracellular effects soon after irradiation. Moreover, the in situ DSB assay may provide a means to evaluate the spatial effect on unirradiated cells that are located in the neighboring region of cells irradiated by alpha particles.
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, People's Republic of China. ljw@ipp.ac.cn
authors.forename: Burong
authors.initials: B
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Lijun
authors.initials: L
authors.lastname: Wu
authors.forename: Huiyun
authors.initials: H
authors.lastname: Feng
authors.forename: Xuelan
authors.initials: X
authors.lastname: Liu
authors.forename: Leilei
authors.initials: L
authors.lastname: Zhang
authors.forename: An
authors.initials: A
authors.lastname: Xu
authors.forename: T K
authors.initials: TK
authors.lastname: Hei
authors.forename: Zengliang
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000512:Alpha Particles / Q000009:adverse effects*; D002460:Cell Line; D002467:Cell Nucleus / Q000528:radiation effects / Q000648:ultrastructure; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D045604:Extracellular Fluid / Q000528:radiation effects; D005347:Fibroblasts / Q000473:pathology* / Q000502:physiology / Q000528:radiation effects*; D005455:Fluorescent Antibody Technique / Q000379:methods; D006657:Histones / Q000235:genetics* / Q000528:radiation effects; D006801:Humans; D009152:Mutagenicity Tests / Q000379:methods*; D011829:Radiation Dosage
chemical_list: C468783:H2AX protein, human; D006657:Histones; D004247:DNA