[Identification of the resource suspected sperm by DNA fingerprinting].
OBJECTIVE
To identify the resource suspected sperm of donor in human sperm bank and apply the parentage testing between the donor and his offspring.


METHODS
We took the 6 semen specimen of the donor involved and correspondently suspected semen as well as the semen of one volunteer and peripheral blood of his offspring. All specimens were amplified by PCR, and DNA fingerprint was detected by PAGE electrophoresis.


RESULTS
By DNA fingerprinting we discovered that the 5 suspected sperm samples came from corresponding donors and the other sample from another. The volunteer and his offspring were identified as consanguinity.


CONCLUSIONS
We can identify the difference between sperm of donors and the suspected sperm of donors, and sperm of donors and the peripheral blood of their offspring exactly by DNA fingerprinting.
Zhu
Wen-bing
WB
Reproduction and Stem Cell Engineering Institute, Central South University, Changsha 410078, China.
Fu
Jun-jiang
JJ
Liu
Shang-feng
SF
Fan
Li-qing
LQ
Li
Lu-yun
LY
Lu
Guang-xiu
GX
D000328:Adult; D003241:Consanguinity; D016172:DNA Fingerprinting; D006801:Humans; D008297:Male; D013074:Sperm Banks; D013094:Spermatozoa / Q000737:chemistry*; D014019:Tissue Donors