[Introduction of a simple and effective method for plant total RNA isolation].
Wheat leaf and seeds at different development stages had been squashed in liquid nitrogen, then lysed by urea buffer which contains 0.1% SDS and 0.1% LDS, denatured protein had been removed by NaAc and chloroform precipitation, total RNA was further purified by LiCl. The RNA we obtained had sharp bands of 28S and 18S after agarose gel electrophoresis, 23S and 16S RNA bands can also be seen clearly in leaf RNA extract, the value of OD260/OD280 of RNA was 2.05-2.105 mg RNA can been isolated from 10 g leaf of wheat. This method can also been used in high molecular weight DNA isolation but the concentration of SDS and LDS must be increased to 1%.
Zhao
Shuang-Yi
SY
School of Life Sciences, Shandong University, Jinan 250100, China. syzhao4123@sina.com
Wu
Yao-Rong
YR
Xia
Guang-Min
GM