title: Comparative radical scavenging and antidiabetic activities of methanolic extract and fractions from Achillea ligustica ALL.
abstract: The yield of methanolic extract and total phenol and non polar content of flowered parts from Achillea ligustica ALL. are reported. GC-MS analysis of the non polar fraction showed that the triterpene moretenol was the major constituent (17.228%) followed by stigmast-6-en-3beta-ol, veridiflorol and beta-amyrin (7.524%, 5.078% 4.470%, respectively). The antioxidant activities of the methanolic extract and its fractions from A. ligustica were carried out using two different in vitro assays, 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and lipid peroxidation of liposomes assay. Methanolic extract showed higher radical scavenging activity on DPPH (IC50 of 50 microg/ml). This activity is probably due to the phenolic fraction which shown an IC50 value of 22 microg/ml. A different result was obtained from the methanolic extract on the lipid peroxidation of liposomes (IC50 of 416 microg/ml). The alpha-amylase inhibition assay was applied to evaluate antidiabetic activity. The methanolic extract showed weak activity (28.18% at 1 mg/ml) while the n-hexane fraction showed 74.96% inhibition at 250 microg/ml.
authors.affiliation: Department of Pharmaceutical Sciences, University of Calabria, I-87036 Rende (CS), Italy. filomena.conforti@unical.it
authors.forename: Filomena
authors.initials: F
authors.lastname: Conforti
authors.forename: Monica Rosa
authors.initials: MR
authors.lastname: Loizzo
authors.forename: Giancarlo Antonio
authors.initials: GA
authors.lastname: Statti
authors.forename: Francesco
authors.initials: F
authors.lastname: Menichini
mesh_terms: D031162:Achillea / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D006834:Hydrazines / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D000432:Methanol; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology; D012997:Solvents; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D006834:Hydrazines; D007004:Hypoglycemic Agents; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; C004931:1,1-diphenyl-2-picrylhydrazyl; D000516:alpha-Amylases; D000432:Methanol