title: Mannheimia (Pasteurella) haemolytica leukotoxin binding domain lies within amino acids 1 to 291 of bovine CD18.
abstract: Previously, we identified bovine CD18 as the receptor for leukotoxin secreted by Mannheimia (Pasteurella) haemolytica. In this study, we constructed bovine-murine CD18 chimeras to locate the leukotoxin binding domain on CD18. Leukotoxin specifically lysed transfectants expressing bovine CD18 fragment encompassing amino acids 1 to 291, indicating that leukotoxin binding domain lies within amino acids 1 to 291 of bovine CD18.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Nebraska--Lincoln, Lincoln, Nebraska 68583-0905, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Gopinath
authors.forename: T C
authors.initials: TC
authors.lastname: Ambagala
authors.forename: M S
authors.initials: MS
authors.lastname: Deshpande
authors.forename: R O
authors.initials: RO
authors.lastname: Donis
authors.forename: S
authors.initials: S
authors.lastname: Srikumaran
mesh_terms: D000818:Animals; D018821:CD18 Antigens / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005098:Exotoxins / Q000235:genetics / Q000378:metabolism*; D016978:Mannheimia haemolytica / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018821:CD18 Antigens; D018076:DNA, Complementary; D005098:Exotoxins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C018579:leukotoxin