title: Spectral and kinetic studies on the interaction between oxyhaemoglobin and peroxynitrite: a comparison with hydrogen peroxide.
abstract: Interaction between oxyhaemoglobin and peroxynitrite was studied using stopped-flow rapid-scan spectrophotometry. The influence of pH, peroxynitrite concentration and temperature on the pseudo-first-order rate constants was studied and the activation energy calculated. The kinetic curve for the oxyhaemoglobin-peroxynitrite reaction showed that a fast reaction occurred in the initial seconds, followed by a slow process of decrease in absorbance. The biphasic reaction kinetics of oxyhaemoglobin with peroxynitrite or hydrogen peroxide demonstrated the existence of an intermediary species. For the first time a rapid-scan stopped-flow spectrophotometry study is presented, yielding spectral and kinetic data of the reaction.
authors.affiliation: Key Laboratory of MOE for Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, People's Republic of China. rywang97@163.com
authors.forename: Rui-yong
authors.initials: RY
authors.lastname: Wang
authors.forename: Mi-na
authors.initials: MN
authors.lastname: Ji
authors.forename: Ru-xiu
authors.initials: RX
authors.lastname: Cai
authors.forename: Ying-tang
authors.initials: YT
authors.lastname: Lu
mesh_terms: D000818:Animals; D002417:Cattle; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007700:Kinetics; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000737:chemistry*; D030421:Peroxynitrous Acid / Q000737:chemistry*; D010777:Photochemistry; D013057:Spectrum Analysis
chemical_list: D010108:Oxyhemoglobins; D030421:Peroxynitrous Acid; D006861:Hydrogen Peroxide