title: Effects of selenium stress on photosynthetic pigment contents and growth of Chlorella vulgaris.
abstract: Changes in photosynthetic pigment and protein contents, growth and the spectral characteristics in Chlorella vulgaris in response to selenium stress were investigated. Carotenoids (beta-carotene and xanthophylls) and chlorophyll (Chl a and Chl b) contents in cells exposed to Se 50 mg/L increased primarily and decreased afterwards, while photosynthetic pigments in cells exposed to Se 800 mg/L decreased significantly. Chlorophyll (Chl) absorption peak at 693 nm and prominent Chl emission peak at 700 nm, weakened significantly after Se stress. The excitation spectra showed a decrease in excitation energy transfer efficiency in Se-stressed cells. Total soluble protein decreased after Se stress. The changes in total Se, Mg(2+), Ca(2+), K(+) and Na(+) concentrations in culture medium and cells were also determined by ICP-AES.
authors.affiliation: Department of Chemistry, Jinan University, China.
authors.forename: Tian-Feng
authors.initials: TF
authors.lastname: Chen
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Zheng
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Bai
authors.forename: Fang
authors.initials: F
authors.lastname: Tu
mesh_terms: D002118:Calcium / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism*; D048408:Chlorella vulgaris / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D002851:Chromatography, High Pressure Liquid; D008274:Magnesium / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012643:Selenium / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism; D024341:Xanthophylls / Q000378:metabolism; D019207:beta Carotene / Q000378:metabolism
chemical_list: D024341:Xanthophylls; D019207:beta Carotene; D002734:Chlorophyll; D002338:Carotenoids; C037184:chlorophyll b; D012964:Sodium; D012643:Selenium; D008274:Magnesium; D011188:Potassium; D002118:Calcium; D000077194:Chlorophyll A