title: Transient activation of protein phosphatase 2A induced by electroconvulsive shock in the rat frontal cortex.
abstract: We have attempted to determine the effects of electroconvulsive shock (ECS) on protein phosphatase 2A (PP2A) in the frontal cortices of rats. PP2A exhibited a 30% increase in activity immediately after ECS treatment. Immunoblot analysis revealed that phosphorylation signals, including protein kinase B (Akt/PKB), glycogen synthase kinase-3beta (GSK-3beta), and cyclic adenosine monophosphate response element binding protein (CREB) were reduced immediately after ECS treatment. When an additional ECS was administered after the activation of these kinases, the immediate reactivation of PP2A overrode the kinase activity. ECS induces transient PP2A activation prior to kinase activation, and this pattern of activity may induce the biphasic phosphorylation of substrate proteins.
authors.affiliation: Department of Psychiatry & Behavioral Science, Seoul National University College of Medicine, Seoul, South Korea.
authors.forename: Ung Gu
authors.initials: UG
authors.lastname: Kang
authors.forename: Won Je
authors.initials: WJ
authors.lastname: Jeon
authors.forename: Yeni
authors.initials: Y
authors.lastname: Kim
authors.forename: Chun Kee
authors.initials: CK
authors.lastname: Chung
authors.forename: Joo Bae
authors.initials: JB
authors.lastname: Park
authors.forename: Yong Sung
authors.initials: YS
authors.lastname: Juhnn
authors.forename: Yong Sik
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D004307:Dose-Response Relationship, Radiation; D004597:Electroshock / Q000379:methods*; D004789:Enzyme Activation / Q000528:radiation effects; D005625:Frontal Lobe / Q000378:metabolism / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000528:radiation effects*; D008297:Male; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation / Q000528:radiation effects; D011494:Protein Kinases / Q000378:metabolism; D054648:Protein Phosphatase 2; D051381:Rats; D017207:Rats, Sprague-Dawley; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism; D013997:Time Factors
chemical_list: D013912:Threonine; D012694:Serine; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2