Extremely low electrical current generated by porcine small intestine smooth muscle alters bacterial autolysin production.
The effect of extremely low electrical currents, identical to those generated by the gut smooth muscle, on bacterial autolysin production in vitro was tested in the present study. When stimulated with these electrical currents, the bacteria Pediococcus pentosaceus 16:1 produced groups of peptidoglycan hydrolases that differed from those produced by the unstimulated (control) bacteria. The autolysins synthesized by the P. pentosaceus 16:1 under extremely low electrical currents were effective against peptidoglycans from the cell walls of various lactic acid bacteria strains, whereas the autolysins from the control bacteria acted exclusively against P. pentosaceus 16:1 cell walls. Thus, it can be predicted that in vivo the electrical currents generated by the intestinal smooth muscles, which can be recorded as the myoelectrical migrating complexes, could regulate lactic acid bacteria strain growth in the gut.
Kruszewska
Danuta
D
Department of Laboratory Medicine, Lund University, Sölvegatan 23, SE-223 62 Lund, Sweden.
Podgurniak
Pawel
P
Ljungh
Asa
A
Sebastian
Aleksandra
A
Larsson
Lennart
L
Zajdel-Dabrowska
Jolanta
J
Pierzynowski
Stefan G
SG
D000818:Animals; D002473:Cell Wall / Q000201:enzymology; D015180:Electrophoresis, Gel, Two-Dimensional; D004594:Electrophysiology; D007421:Intestine, Small / Q000382:microbiology* / Q000502:physiology; D007778:Lactobacillus / Q000502:physiology; D007934:Leuconostoc / Q000502:physiology; D009130:Muscle, Smooth / Q000502:physiology*; D016065:Myoelectric Complex, Migrating / Q000502:physiology*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000096:biosynthesis*; D010376:Pediococcus / Q000502:physiology*; D010457:Peptidoglycan / Q000378:metabolism; D013552:Swine
D010457:Peptidoglycan; D009238:N-Acetylmuramoyl-L-alanine Amidase