Slime production and proteinase activity of Candida species isolated from blood samples and the comparison of these activities with minimum inhibitory concentration values of antifungal agents.
Slime and proteinase activity of 54 strains consisting of 19 Candida parapsilosis and 35 C. albicans strains isolated from blood samples were investigated in this study. Ketoconazole, amphothericin B, and fluconazole susceptibility of Candida species were compared with slime production and proteinase activity of these species. For both Candida species, no correlation was detected between the slime activity and minimum inhibitory concentration (MIC) values of the three antifungal agents. For both Candida species no correlation was detected between the proteinase activity and the MIC values of amphothericin B, and fluconazole however, statistically significant difference, was determined between the proteinase activity and MIC values of ketoconazole (p = 0.007). Slime production was determined by using modified Christensen macrotube method and proteinase activity was measured by the method of Staib. Antifungal susceptibility was determined through the guidelines of National Committee for Laboratory Standards (NCCLS M27-A).
Ozkan
Semiha
S
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Gazi University, Ankara, Turkey. sozkan@gazi.edu.tr
Kaynak
Fatma
F
Kalkanci
Ayse
A
Abbasoglu
Ufuk
U
Kustimur
Semra
S
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002175:Candida / Q000187:drug effects* / Q000201:enzymology; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D007654:Ketoconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010447:Peptide Hydrolases / Q000187:drug effects / Q000378:metabolism*
D000935:Antifungal Agents; D000666:Amphotericin B; D015725:Fluconazole; D010447:Peptide Hydrolases; D007654:Ketoconazole