[The insect resistance of OC and Bt transplastomic plant and the phenotype of their progenies].
The Bt gene and OC gene were cotransformed to tobacco chloroplast with particle bombardment method and spectinomycin resistance tobacco seedlings were obtained. Bioassays showed that the transgenic tobacco containing both genes had enhanced toxicity to the larvae of cotton bollworm (helicoverpa zea) by comparison with the plants containing only Bt or OC gene. Southern-blotting analysis and genetic analysis of progenies showed that the Bt and OC gene expressed and was inherited maternally to the progenies.
Su
Ning
N
Biotechnology Research Institute, CAAS, Beijing 100081, China. sn68@sina.com
Sun
Meng
M
Yang
Bo
B
Meng
Kun
K
Liu
Chun-Ying
CY
Ni
Pi-Chong
PC
Shen
Gui-Fang
GF