Electrospinning of collagen nanofibers: effects on the behavior of normal human keratinocytes and early-stage wound healing.
Electrospinning of type I collagen in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) to fabricate a biomimetic nanofibrous extracellular matrix for tissue engineering was investigated. The average diameter of collagen nanofibers electrospun from 8% collagen solution in HFIP was 460 nm (range of 100-1200 nm). The as-spun collagen nanofibrous matrix was chemically cross-linked by glutaraldehyde vapor with a saturated aqueous solution and then treated with aqueous 0.1m glycine to block unreacted aldehyde groups. With vapor phase cross-linking for 12h, porosity of the collagen matrix decreased from 89% to 71%. The collagen nanofibrous matrix showed good tensile strength, even in aqueous solution. Effects on cytocompatibility, cell behavior, cell and collagen nanofiber interactions, and open wound healing in rats were examined. Relatively low cell adhesion was observed on uncoated collagen nanofibers, whereas collagen nanofibrous matrices treated with type I collagen or laminin were functionally active in responses in normal human keratinocytes. Collagen nanofibrous matrices were very effective as wound-healing accelerators in early-stage wound healing. Our results indicate that cross-linked collagen nanofibers coated with ECM proteins, particularly type I collagen, may be a good candidate for biomedical applications, such as wound dressing and scaffolds for tissue engineering.
Rho
Kyong Su
KS
Department of Oral Biochemistry and Craniomaxillofacial Reconstructive Science, Dental Research Institute, and BK21 HLS, Seoul National University College of Dentistry, Seoul 110-749, South Korea.
Jeong
Lim
L
Lee
Gene
G
Seo
Byoung-Moo
BM
Park
Yoon Jeong
YJ
Hong
Seong-Doo
SD
Roh
Sangho
S
Cho
Jae Jin
JJ
Park
Won Ho
WH
Min
Byung-Moo
BM
D000328:Adult; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D024042:Collagen Type I; D005109:Extracellular Matrix; D006801:Humans; D015603:Keratinocytes / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D036103:Nanotechnology / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000473:pathology; D014945:Wound Healing / Q000502:physiology*
D001672:Biocompatible Materials; D024042:Collagen Type I