Comparative radical scavenging and antidiabetic activities of methanolic extract and fractions from Achillea ligustica ALL.
The yield of methanolic extract and total phenol and non polar content of flowered parts from Achillea ligustica ALL. are reported. GC-MS analysis of the non polar fraction showed that the triterpene moretenol was the major constituent (17.228%) followed by stigmast-6-en-3beta-ol, veridiflorol and beta-amyrin (7.524%, 5.078% 4.470%, respectively). The antioxidant activities of the methanolic extract and its fractions from A. ligustica were carried out using two different in vitro assays, 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and lipid peroxidation of liposomes assay. Methanolic extract showed higher radical scavenging activity on DPPH (IC50 of 50 microg/ml). This activity is probably due to the phenolic fraction which shown an IC50 value of 22 microg/ml. A different result was obtained from the methanolic extract on the lipid peroxidation of liposomes (IC50 of 416 microg/ml). The alpha-amylase inhibition assay was applied to evaluate antidiabetic activity. The methanolic extract showed weak activity (28.18% at 1 mg/ml) while the n-hexane fraction showed 74.96% inhibition at 250 microg/ml.
Conforti
Filomena
F
Department of Pharmaceutical Sciences, University of Calabria, I-87036 Rende (CS), Italy. filomena.conforti@unical.it
Loizzo
Monica Rosa
MR
Statti
Giancarlo Antonio
GA
Menichini
Francesco
F
D031162:Achillea / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D006834:Hydrazines / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D000432:Methanol; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology; D012997:Solvents; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D006834:Hydrazines; D007004:Hypoglycemic Agents; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; C004931:1,1-diphenyl-2-picrylhydrazyl; D000516:alpha-Amylases; D000432:Methanol