Establishment and characterization of human malignant lymphoma cell line derived from uterine cervix.
Human uterine cervical malignant lymphoma (B-cell type) was cultured and the cell line (HIUML) was newly established. The HIUML cells were round in shape and had a tendency to make floating clusters. The cells had a smooth surface or protrusion on the margin of the cytoplasm, and proliferate in floatation. The population doubling time was about 32 hours and 42 or more passages were successfully observed in two years. The HIUML cells were not transplantable into nude mice but were successfully done in the cheek pouch of hamster with formation of malignant lymphoma. Epstein-Barr virus was detected in the HIUML cells.
Kiguchi
Kazushige
K
Department of Obstetrics and Gynecology, St.Marianna University School of Medicine.
Ishiwata
Isamu
I
Ishiwata
Chieko
C
Ishiwata
Emiko
E
Koshitaka
Yutaka
Y
Iwata
Masanori
M
Okuda
Yoshiko
Y
Kobayashi
Youichi
Y
Wada
Yasuna
Y
Ishizuka
Bunpei
B
Ishikawa
Hiroshi
H
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006224:Cricetinae; D005260:Female; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D007621:Karyotyping; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000473:pathology* / Q000821:virology; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D013997:Time Factors; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology* / Q000821:virology
D015703:Antigens, CD