Ceramide-1-P induces Ca2+ mobilization in Jurkat T-cells by elevation of Ins(1,4,5)-P3 and activation of a store-operated calcium channel.
Sphingolipids comprise a very important class of second messengers involved in cell growth, differentiation, and apoptosis, among other different functions. Recently, these lipids have been implicated in calcium mobilization in different cell lines, including Jurkat T-lymphocytes. However, the effect of each particular sphingolipid appears to be cell-line specific. Among them, the least studied is ceramide-1-P (Cer-1-P). Here, we show that Cer-1-P increased the intracellular Ca(2+) concentration in Jurkat T-cells. Furthermore, laser-scanning confocal microscopy indicated that Ca(2+) is released from the endoplasmic reticulum. An effect on store-operated Ca(2+) channels was evidenced by whole-cell "patch clamp" measurements after Cer-1-P induced Ca(2+) store depletion. The mechanism of action of Cer-1-P resembles that of the Jurkat anti-TCR antibody, but differs from that of ceramide, since Cer-1-P induced an increase in Ins(1,4,5)-P(3).
Colina
Claudia
C
Laboratorio de Permeabilidad Iónica, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.
Flores
Adriana
A
Castillo
Cecilia
C
Garrido
María del Rosario
Mdel R
Israel
Anita
A
DiPolo
Reinaldo
R
Benaim
Gustavo
G
D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism*; D002518:Ceramides / Q000494:pharmacology*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D019169:Jurkat Cells; D018408:Patch-Clamp Techniques; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism
D015220:Calcium Channels; D002518:Ceramides; C065576:ceramide 1-phosphate; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium