title: Correlating structure with solute and water transport in a chronic model of peritoneal inflammation.
abstract: To study the process of chronic peritoneal inflammation from sterile solutions, we established an animal model to link structural changes with solute and water transport. Filtered solutions containing 4% N-acetylglucosamine (NAG) or 4% glucose (G) were injected intraperitoneally daily in 200- to 300-g rats and compared with controls (C). After 2 mo, each animal underwent transport studies using a chamber affixed to the parietal peritoneum to determine small-solute and protein mass transfer, osmotic filtration, and hydraulic flow. After euthanasia, parietal tissues were sampled for histological analysis, which demonstrated significant differences in peritoneal thickness (microm; C, 42.6 +/- 7.5; G, 80.4 +/- 22.3; NAG, 450 +/- 104; P < 0.05). Staining for VEGF correlated with CD-31 vessel counts (no./mm2: C, 53.1 +/- 16.1; G, 166 +/- 32; NAG, 183 +/- 32; P < 0.05). Tissue analysis showed treatment effects on tissue hyaluronan (micro/g: C, 962 +/- 73; G, 1,169 +/- 69; NAG, 1,428 +/- 69; P < 0.05) and collagen (microg/g: C, 56.9 +/- 12.0; G, 107 +/- 12; NAG, 97.6 +/- 11.4; P < 0.05) but not sulfated glycosaminoglycan. Transport experiments revealed no significant differences in mannitol transfer or osmotic flow. Changes were seen in hydrostatic pressure-driven flux (microl x min(-1) x cm(-2): C, 0.676 +/- 0.133; G, 0.317 +/- 0.124; NAG, 0.284 +/- 0.117; P < 0.05) and albumin transfer (microl x min(-1) x cm(-2): C, 0.331 +/- 0.028; G, 0.286 +/- 0.026; NAG, 0.229 +/- 0.025; P < 0.04). We conclude that alteration of the interstitial matrix correlates with diminished hydraulic conductivity and macromolecular transport.
authors.affiliation: Nephrology Div., Dept. of Medicine, Univ. of Mississippi Medical Ctr., 2500 North State St., Jackson, MS 39216-4505, USA. mflessner@medicine.umsmed.edu
authors.forename: Michael F
authors.initials: MF
authors.lastname: Flessner
authors.forename: Jaehwa
authors.initials: J
authors.lastname: Choi
authors.forename: Heather
authors.initials: H
authors.lastname: Vanpelt
authors.forename: Zhi
authors.initials: Z
authors.lastname: He
authors.forename: Kimberly
authors.initials: K
authors.lastname: Credit
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Henegar
authors.forename: Michael
authors.initials: M
authors.lastname: Hughson
mesh_terms: D034861:Abdominal Wall / Q000473:pathology; D000818:Animals; D001692:Biological Transport; D003094:Collagen / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D006820:Hyaluronic Acid / Q000378:metabolism*; D008954:Models, Biological; D010530:Peritoneal Dialysis / Q000379:methods; D010537:Peritoneum / Q000378:metabolism*; D010538:Peritonitis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water; D006820:Hyaluronic Acid; D003094:Collagen