Multiparametric assay to screen and dissect the mode of action of anti-human immunodeficiency virus envelope drugs.
A flow cytometry-based assay was used to simultaneously quantify X4 and R5 human immunodeficiency virus (HIV) envelope-mediated cell-to-cell viral transfer, cell death, and cell-to-cell fusion. In this assay, different anti-HIV envelope drugs showed characteristic inhibitory profiles for each measured parameter, allowing for the rapid identification of the mode of action of active compounds.
Blanco
Julià
J
Fundació irsiCaixa, Hospital Universitari Germans Trias i Pujol, 08916 Badalona, Catalonia, Spain. jblanco@irsicaixa.es
Clotet-Codina
Imma
I
Bosch
Berta
B
Armand-Ugón
Mercedes
M
Clotet
Bonaventura
B
Esté
José A
JA
D019380:Anti-HIV Agents / Q000494:pharmacology*; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D002459:Cell Fusion; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D015658:HIV Infections / Q000635:transmission*; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D013778:Tetrazolium Salts; D013844:Thiazoles; D014759:Viral Envelope Proteins / Q000037:antagonists & inhibitors* / Q000187:drug effects
D019380:Anti-HIV Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D014759:Viral Envelope Proteins; C022616:thiazolyl blue