title: Five new triterpene glycosides from Lysimachia foenum-graecum and evaluation of their effect on the arachidonic acid metabolizing enzyme.
abstract: Five new oleanane-type triterpene saponins, named foenumosides A ( 1), B ( 2), C ( 3), D ( 4) and E ( 5), were isolated from the aerial parts of Lysimachia foenum-graecum Hance. Their structures were identified on the basis of 1D and 2D NMR techniques, including H-H COSY, HMQC, HMBC, HMQC-TOCSY, ROESY experiments as well as chemical methods. We have evaluated the cytotoxity of 1 - 5 against rat and human polymorphonuclear leukocytes and the effect of 5 on the arachidonic acid metabolizing enzyme. All compounds showed a high degree of toxicity except for compound 5, while 5 notably reduced the production of leukotriene B (4) (LTB (4)) from rat peritoneal leukocytes with an IC (50) value of 74 microM without inhibiting human elastase. Compound 5 also reduced the production of 12-HHTrE and 12-HETE by 14 % and 50 % as a measurement for cyclooxygenase-1 and 12-lipoxygenase inhibition at 100 microM.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China.
authors.forename: Yun-Heng
authors.initials: YH
authors.lastname: Shen
authors.forename: Zhi-Ying
authors.initials: ZY
authors.lastname: Weng
authors.forename: Qin-Shi
authors.initials: QS
authors.lastname: Zhao
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Zeng
authors.forename: José-Luis
authors.initials: JL
authors.lastname: Ríos
authors.forename: Wei-Lie
authors.initials: WL
authors.lastname: Xiao
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Han-Dong
authors.initials: HD
authors.lastname: Sun
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D006027:Glycosides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D020128:Inhibitory Concentration 50; D007962:Leukocytes / Q000187:drug effects / Q000201:enzymology; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D029623:Primulaceae; D051381:Rats; D014315:Triterpenes / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D006027:Glycosides; D010936:Plant Extracts; D014315:Triterpenes; D016718:Arachidonic Acid