Simple and effective technical procedures for the packing and application of nanospray PicoFrit HPLC columns for proteomic analyses.
A simple procedure was developed for packing PicoFrit HPLC columns with chromatographic stationary phase using a reservoir fabricated from standard laboratory HPLC fittings. Packed columns were mounted onto a stainless steel ultra-low volume precolumn filter assembly containing a 0.5-microm pore size steel frit. This format provided a conduit for the application of the nanospray voltage and protected the column from obstruction by sample material. The system was characterised and operational performance assessed by analysis of a range of peptide standards (n = 9).
Howells
Laurence C
LC
Department of Transmissible Molecular Biology, Veterinary Laboratories Agency, Addlestone, Surrey, UK.
Whelan
Adam O
AO
Woodward
Martin J
MJ
Coldham
Nick G
NG
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D040901:Proteomics / Q000295:instrumentation
D010455:Peptides