Sensitive determination of D-amino acids in mammals and the effect of D-amino-acid oxidase activity on their amounts.
The determination of small amounts of D-amino acids in mammalian tissues is still a challenging theme in the separation sciences. In this review, various gas-chromatographic and high-performance liquid chromatographic methods are discussed including highly selective and sensitive column-switching procedures. Based on these methods, the distributions of D-aspartic acid, D-serine, D-alanine, D-leucine and D-proline have been clarified in the mouse brain. As the regulation mechanisms of D-amino acid amounts in mammals, we focused on the D-amino-acid oxidase, which catalyzes the degradation of D-amino acids. Using the mutant mouse strain lacking D-amino-acid oxidase activity, the effects of the enzymatic activity on the amounts and distributions of various D-amino acids have been investigated.
Hamase
Kenji
K
Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Konno
Ryuichi
R
Morikawa
Akiko
A
Zaitsu
Kiyoshi
K
D000596:Amino Acids / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D003605:D-Amino-Acid Oxidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008322:Mammals / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout
D000596:Amino Acids; D003605:D-Amino-Acid Oxidase