Bone tissue engineering evaluation based on rat calvaria stromal cells cultured on modified PLGA scaffolds.
Using natural materials to coat the scaffolds used for tissue-engineered bone-repair techniques is expected to increase osteoblast adhesion to the scaffold and to express normal physiological function. To test this hypothesis, we therefore modified poly(DL-lactic-co-glycolic acid) (PLGA) substrate by coating it with natural biomaterial solutions of collagen, chitosan, or N-succinyl-chitosan, and then used these three combinations as scaffolds to evaluate their effects on osteoblast attachment, proliferation, and differentiation. The results demonstrated that the pore size of scaffolds ranging from 125-500 microm did not affect the osteoblast phenotype; however, the surface modification of the scaffolds coated with these natural biomaterials did. Collagen increased cell attachment and proliferation, but chitosan and N-succinyl-chitosan decreased them. Chitosan and N-succinyl-chitosan increased differentiation, but collagen decreased it. These results provide us a new strategy for modifying microenvironments to increase osteoblast adhesion, proliferation, and differentiation on PLGA scaffolds, a strategy that might be useful for tissue regeneration.
Wu
Yu-Chun
YC
Institute of Biotechnology, National Cheng Kung University, Tainan 70428, Taiwan.
Shaw
Shyh-Yu
SY
Lin
Hong-Ru
HR
Lee
Tzer-Min
TM
Yang
Chyun-Yu
CY
D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002478:Cells, Cultured; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D012886:Skull / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology*; D023822:Tissue Engineering; D014867:Water / Q000737:chemistry
D011108:Polymers; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid