Expression and characterization of vanilloid receptor subtype 1 in human dental pulp cell cultures.
The expression of the vanilloid receptor subtype 1 (VR1, TRPV1) was detected in human dental pulp fibroblasts (PF-10) using RT-PCR, Western blotting, and immunocytochemical analysis. As revealed by ELISA, capsaicin induced IL-6 expression in PF-10 cells, and the VR1 antagonist capsazepine dose-dependently inhibited capsaicin-induced IL-6 production, indicating that capsaicin-induced IL-6 expression is related to VR1 activation. The interaction between capsaicin and mitogen-activated protein kinases (MAPKs) was investigated. The phosphorylation of p38 MAPK and c-Jun NH2-terminal kinase (JNK) were detected after capsaicin stimulation. p38 MAPK is involved in capsaicin-induced IL-6 production, as shown by the use of specific inhibitors of this kinase. The result of EMSA showed that capsaicin inhibited tumor necrosis factor-alpha (TNF-alpha)-induced nuclear factor-kappa B (NF-kappaB) activation in PF-10 cell cultures. These results suggest that the activation of VR1 plays an important role in dental pulp inflammation.
Miyamoto
Rie
R
Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Tokuda
Masayuki
M
Sakuta
Tetsuya
T
Nagaoka
Shigetaka
S
Torii
Mitsuo
M
D000328:Adult; D002211:Capsaicin / Q000378:metabolism; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000503:physiopathology*; D005260:Female; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D011955:Receptors, Drug / Q000378:metabolism*; D050916:TRPV Cation Channels
D016328:NF-kappa B; D011955:Receptors, Drug; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; D020928:Mitogen-Activated Protein Kinases; D002211:Capsaicin