title: Relationship between inflammatory cytology of canine seminal fluid and significant aerobic bacterial, anaerobic bacterial or mycoplasma cultures of canine seminal fluid: 95 cases (1987-2000).
abstract: Seminal fluid was collected by manual ejaculation from 95 dogs. Quantitative aerobic bacterial, qualitative anaerobic bacterial and mycoplasma cultures were performed on the seminal fluid, and their association with presence of inflammatory cells present in the pellet formed after centrifugation of the fluid was investigated. There was a clinically meaningful aerobic bacterial growth in 28.4%, anaerobic bacterial growth in 13.7%, and mycoplasma growth in 57.9% of the seminal fluid samples. Presence of inflammatory cytology was statistically associated with clinically meaningful aerobic bacterial growth. However, of the 78 dogs (82.1%) with clinically meaningful growth of at least one aerobic, anaerobic or mycoplasma organism, 43 (55.1%) had non-inflammatory seminal fluid cytology.
authors.affiliation: University of Minnesota, Department of Veterinary Clinical Sciences, St. Paul, MN 55108, USA. rootk001@umn.edu
authors.forename: Margaret V Root
authors.initials: MV
authors.lastname: Kustritz
authors.forename: Shirley D
authors.initials: SD
authors.lastname: Johnston
authors.forename: Patricia N
authors.initials: PN
authors.lastname: Olson
authors.forename: Cindy J
authors.initials: CJ
authors.lastname: Lindeman
mesh_terms: D000818:Animals; D001420:Bacteria, Aerobic / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D001947:Breeding; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D007249:Inflammation / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D009174:Mycoplasma / Q000302:isolation & purification; D012189:Retrospective Studies; D012661:Semen / Q000166:cytology* / Q000382:microbiology*