[Study on degradable properties of 3D-SC artificial skin in vitro].
OBJECTIVE
To study the degradable properties of 3D-SC artificial skin in vitro.


METHODS
The 3D-SC artificial skin materials were respectively immersed into the solutions of 0.9% normal saline (control group), pancreatic tissue liquid (experimental group 1), physiological buffer (Hanks balanced salt solution,experimental group 2) and 0.2 mol/L phosphate buffer (pH 7.4, experimental group 3), and the degradation was carried out at 37 degrees C. The quality lost ratios were determined on the 3rd day, the 5th day, the 7th day, the 9th day, 11th day and 14th day in the experimental group 1, while on the 3rd day, 7th day, 14th day, 15th day, 21st day and 30th day in the other groups.


RESULTS
The 3D-SC artificial skin was degraded completely in pancreatic tissue liquid about within 14 days in the experimental group 1; in the control group, and in the experimental groups 2 and 3, the degradation ratios were 8.68% +/- 2.30%, 28.51% +/- 10.68% and 7.35% +/- 0.61% on the 14th day; 71.83% +/- 2.58%, 91.32% +/- 1.87% and 75.64% +/- 6.13% on the 15th day, being significant difference between the control group and the experimental group 2 (P < 0.01); and 91.87% +/- 8.15%, 95.62% +/- 1.36% and 92.10% +/- 2.26% on the 30th day, being no significant differences between these 3 groups(P > 0.05), respectively.


CONCLUSIONS
The 3D-SC artificial skin materials have good degradable properties. The trend of degradation speed is from slow to quick and then to slow without enzyme.
Wang
Kunyu
K
Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu Sichuan 610065, P R China.
Dan
Weihua
W
Li
Zhiqiang
Z
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D017768:Skin, Artificial; D013552:Swine; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials