Effect of fasting on nychthemeral concentrations of plasma growth hormone (GH), insulin-like growth factor I (IGF-I), and cortisol in channel catfish (Ictalurus punctatus).
This experiment was conducted to characterize the effect of fasting versus satiety feeding on plasma concentrations of GH, IGF-I, and cortisol over a nychthemeron. Channel catfish fingerlings were acclimated for two weeks under a 12L:12D photoperiod, then fed or fasted for 21 d. On day 21, blood samples were collected every 2 h for 24 h. Weight of fed fish increased an average of 66.2% and fasted fish lost 21.7% of body weight on average. Average nychthemeral concentrations of plasma GH were not significantly different between fed (24.7 ng/mL) and fasted (26.8 ng/mL) fish, but average nychthemeral IGF-I concentrations were higher in fed (23.4 ng/mL) versus fasted (17.8 ng/mL) fish. An increase in plasma IGF-I concentrations was observed in fasted fish 2 h after a peak in plasma GH, but not in fed fish. Average nychthemeral plasma cortisol concentrations were higher in fed (14.5 ng/mL) versus fasted (11.0 ng/mL) fish after 21 d. Significant fluctuations and a postprandial increase in plasma cortisol were observed in fed fish and there was an overall increase in plasma cortisol of both fasted and fed fish during the scotophase. The present experiment indicates little or no effect of 21-d fasting on plasma GH levels but demonstrates fasting-induced suppression of plasma IGF-I and cortisol levels in channel catfish.
Small
Brian C
BC
USDA/ARS Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, P.O. Box 38, Stoneville, MS 38776, USA. bsmall@ars.usda.gov
D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D005508:Food Deprivation / Q000502:physiology*; D013006:Growth Hormone / Q000097:blood / Q000378:metabolism*; D006854:Hydrocortisone / Q000097:blood / Q000378:metabolism*; D007059:Ictaluridae / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D006854:Hydrocortisone