[Genetic analysis and identification of three groups three-line hybrid rice and their parents by RAPD markers].
A total of 248 arbitrary 10-mer oligonucleotide primers were screened using RAPD (random amplified polymorphic DNA) techniques with the genome DNA of three groups of three-line hybrid rice and their parents. Thirteen primers produced 43 polymorphism fragments. Six primers of them produced 20 obviously repeatable polymorphic markers among rice lines tested. Using this RAPD markers,the hybrid rice combinations (sterile-line, maintainer-line, restorer-line and F1) can be effectively identified, and the genetic relationship among them can be shown.
Mao
Jia-Ning
JN
The Key Laboratory of MOE for Plant Development Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Duan
Shi-Hua
SH
Li
Shao-Qing
SQ
Zhu
Ying-Guo
YG