Hepatitis B virus X protein interacts with beta5 subunit of heterotrimeric guanine nucleotide binding protein.
BACKGROUND
To isolate cellular proteins interacting with hepatitis B virus X protein (HBX), from HepG2 cells infected with hepatitis B virus (HBV).


RESULTS
HBV particles were produced in culture medium of HepG2 cells transfected with the mammalian expression vector containing the linear HBV genome, as assessed by commercially available ELISA assay. A cDNA library was made from these cells exposed to HBV. From yeast two hybrid screening with HBX as bait, human guanine nucleotide binding protein beta subunit 5L (GNbeta5) was isolated from the cDNA library constructed in this study as a new HBX-interacting protein. The HBX-GNbeta5 interaction was further supported by mammalian two hybrid assay.


CONCLUSIONS
The use of a cDNA library constructed from HBV-transfected HepG2 cells has resulted in the isolation of new cellular proteins interacting with HBX.
Lwa
Siew Hui
SH
School of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, Blk 1 Innovation Centre, 637722, Singapore. SHLwa@yahoo.com
Chen
Wei Ning
WN
D002460:Cell Line; D015723:Gene Library; D006515:Hepatitis B virus / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D025941:Protein Interaction Mapping; D021122:Protein Subunits / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D054334:Viral Regulatory and Accessory Proteins / Q000378:metabolism*
D021122:Protein Subunits; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D020962:Heterotrimeric GTP-Binding Proteins