[Cancer procoagulant and cathepsin D activity in blood serum in patients with bladder cancer].
The increasing morbidity and mortality rates of bladder cancer forced the scientists to search for new unfailing diagnostic and therapeutic methods that will improve treatment effects. There are biochemical cancer markers as cancer procoagulant (CP) and cathepsin D which may be used to this end. The aim of the study was to evaluate the activity of the cancer procoagulant and cathepsin D in the blood serum in patients with superficial bladder cancer. The venous blood samples were from 15 patients with microscopically proved superficial bladder carcinoma (i.e. study group) and 15 normal volunteers as a control group. The serum blood CP activity was determined by the Gordon-Benson's coagulation method and expressed by the clotting time in seconds (s) while the cathepsin D activity was determined by the Folin-Ciocalteau's method and expressed by a quantity of released tyrosine in nmol/ml per 4 hours. The CP activity in serum of patients with superficial bladder cancer was increased in statistically way as compared to the non-cancer controls (p<0.0001). The cathepsin D activity in blood serum of the study group was also enhanced as compared to the control group and the said values differed statistically (p<0.0351). It appears to be justifiable to apply the determination of the CP and cathepsin D activity in blood serum for the diagnostics of superficial bladder cancer.
Szajda
Sławomir Dariusz
SD
Samodzielna Pracownia Biofarmacji.
Darewicz
Barbara
B
Kudelski
Jacek
J
Chlabicz
Marcin
M
Domel
Tomasz
T
Chabielska
Ewa
E
Skrzydlewski
Zdzisław
Z
D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D002277:Carcinoma / Q000097:blood*; D016022:Case-Control Studies; D002402:Cathepsin D / Q000097:blood*; D003546:Cysteine Endopeptidases / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D001749:Urinary Bladder Neoplasms / Q000097:blood*
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant; D002402:Cathepsin D