Analysis of yeast lipids.
The precise quantitative determination of the different lipid classes in mutant cells is key to understand the possible role of the respective gene product in lipid homeostasis. In this chapter, we describe methods based on thin-layer chromatography that are employed routinely to determine the level and relative composition of the major lipid classes from yeast.
Schneiter
Roger
R
Division of Biochemistry, University of Fribourg, Switzerland.
Daum
Gunther
G
D002855:Chromatography, Thin Layer / Q000379:methods*; D020404:Glycerophospholipids / Q000032:analysis / Q000145:classification; D008055:Lipids / Q000032:analysis* / Q000145:classification; D009172:Mycology / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
D020404:Glycerophospholipids; D008055:Lipids