title: Extraction of chitin from red crab shell waste by cofermentation with Lactobacillus paracasei subsp. tolerans KCTC-3074 and Serratia marcescens FS-3.
abstract: For one-step extraction of chitin from red crab shell waste, cofermentation with Lactobacillus paracasei subsp. tolerans KCTC-3074, a lactic-acid-producing bacterium, and Serratia marcescens FS-3, a protease-producing bacterium, was conducted. Fermentation with single strain (L. 3074 or FS-3) was also conducted. At day 7, the pH in L. 3074, FS-3, and L. 3074+FS-3 (1:1) treatment decreased from 6.90 to 3.30, 5.88, and 3.48, respectively. Ash content in the residue after fermentation treatment of crab shells in L. 3074 and L. 3074+FS-3 (1:1) treatment drastically decreased from 41.2% to 3.19 and 1.15%, respectively. In L. 3074+FS-3 (1:1) cofermentation, the level of demineralization was the highest value of 97.2%, but the level of deproteinization in the cofermentation was 52.6% at day 7. Protein content in the treatment of FS-3 alone reduced from 22.4 to 3.62%. These results indicate that cofermentation of the shells using the two strains is efficient and applicable for the one-step extraction of crude chitin from red crab shell waste.
authors.affiliation: Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju, South Korea.
authors.forename: W J
authors.initials: WJ
authors.lastname: Jung
authors.forename: G H
authors.initials: GH
authors.lastname: Jo
authors.forename: J H
authors.initials: JH
authors.lastname: Kuk
authors.forename: K Y
authors.initials: KY
authors.lastname: Kim
authors.forename: R D
authors.initials: RD
authors.lastname: Park
mesh_terms: D000818:Animals; D018533:Biomass; D003386:Brachyura / Q000737:chemistry* / Q000378:metabolism; D002686:Chitin / Q000302:isolation & purification*; D005285:Fermentation; D007780:Lacticaseibacillus casei / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D012706:Serratia marcescens / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002686:Chitin