Establishment and characterization of a human malignant choroids plexus papilloma cell line (HIBCPP).
A cell line designated "HIBSPP" was established from a human malignant choroids plexus papilloma of 29-year-old Japanese woman. This line grew well without interruption for 3 years and was subcultivated over 70 times. The cells were spindle, oval, and polygonal in shape, and neoplastic and pleomorphic features, a jigsaw puzzle-like arrangement, multilayering and forming papillary structures without contact inhibition. The cells proliferated slowly, and the population doubling time was about 69 hours. The chromosome number showed a wide distribution of aneuploidy. The mode was in the hypotetraploid range, and many marker chromosomes were observed. The culture cells were easily transplanted into the subcutis of nude mice and produced the tumor resembling the original tumor.
Ishiwata
Isamu
I
Ishiwata Obstetric and Gynecologic Hospital.
Ishiwata
Chieko
C
Ishiwata
Emiko
E
Sato
Yoshiro
Y
Kiguchi
Kazushige
K
Tachibana
Toshiaki
T
Hashimoto
Hisashi
H
Ishikawa
Hiroshi
H
D000328:Adult; D000782:Aneuploidy; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016545:Choroid Plexus Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D003470:Culture Media / Q000737:chemistry; D005260:Female; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013997:Time Factors
D000951:Antigens, Neoplasm; D003470:Culture Media