title: In vitro biological performances of phosphorylcholine-grafted ePTFE prostheses through RFGD plasma techniques.
abstract: Arterial prostheses made of microporous Teflon (ePTFE) are currently used in vascular surgery as bypasses for small and medium vessels. However, several clinical complications, such as thrombosis, frequently occur in these prostheses when implanted in humans. In this work, an original strategy was developed to improve the hemocompatibility of ePTFE prostheses, based on glow-discharge surface modification followed by chemical grafting of phosphorylcholine, known for its hemocompatible properties. This procedure leads to a covalent attachment of the molecules, therefore preventing their removal by shear stress induced by blood flow at the implant wall. The improvement of the blood compatibility properties of the modified ePTFE arterial prostheses have been investigated by in vitro tests such as thromboelastography, neutrophil adsorption, platelet aggregation, and cell cultures. These in vitro tests put in evidence that thrombogenicity index, platelet aggregation, and neutrophil adhesion were decreased by the molecule grafted on the prostheses. Moreover, the cell growth on the surface of the PRC-grafted prostheses was greatly enhanced in comparison to the virgin prosthesis. Based on these results, it could be concluded that PRC grafting on ePTFE prostheses permit to improve in vitro hemocompatibility and biocompatibility in comparison with their virgin counterpart.
authors.affiliation: Unité de Biotechnologie et de Bioingénierie, Centre de Recherche de l'Hôpital Saint-François d'Assise, CHUQ, 10 rue de l'Espinay, Québec G1L 3L5, Canada.
authors.forename: Pascale
authors.initials: P
authors.lastname: Chevallier
authors.forename: Richard
authors.initials: R
authors.lastname: Janvier
authors.forename: Diego
authors.initials: D
authors.lastname: Mantovani
authors.forename: Gaétan
authors.initials: G
authors.lastname: Laroche
mesh_terms: D000641:Ammonia / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007477:Ions / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D009504:Neutrophils / Q000187:drug effects; D010767:Phosphorylcholine / Q000138:chemical synthesis / Q000737:chemistry*; D010974:Platelet Aggregation; D011138:Polytetrafluoroethylene / Q000737:chemistry / Q000494:pharmacology*; D018629:Spectrometry, Mass, Secondary Ion; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013916:Thrombelastography
chemical_list: D001672:Biocompatible Materials; D007477:Ions; D010767:Phosphorylcholine; D000641:Ammonia; D011138:Polytetrafluoroethylene