title: Pseudoazurin-nitrite reductase interactions.
abstract: The nitrite reductase-binding site on pseudoazurin has been determined by using NMR chemical-shift perturbations. It comprises residues in the hydrophobic patch surrounding the exposed copper ligand His81 as well as several positively charged residues. The binding site is similar for both redox states of pseudoazurin, despite differences in the binding mode. The results suggest that pseudoazurin binds in a well-defined orientation. Docking simulations provide a putative structure of the complex with a binding site on nitrite reductase that has several hydrophobic and polar residues as well as a ridge of negatively charged side chains and a copper-to-copper distance of 14 A.
authors.affiliation: Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300RA Leiden, The Netherlands.
authors.forename: Antonietta
authors.initials: A
authors.lastname: Impagliazzo
authors.forename: Ludwig
authors.initials: L
authors.lastname: Krippahl
authors.forename: Marcellus
authors.initials: M
authors.lastname: Ubbink
mesh_terms: D001400:Azurin / Q000737:chemistry* / Q000378:metabolism*; D003300:Copper / Q000737:chemistry; D008958:Models, Molecular; D009572:Nitrite Reductases / Q000737:chemistry* / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D020836:Protein Structure, Quaternary
chemical_list: C047163:pseudoazurin; D001400:Azurin; D003300:Copper; D009572:Nitrite Reductases