[Influence of 1.8 GHz microwave on DNA damage induced by 4 chemical mutagens].
OBJECTIVE
To observe the influence of 1.8 GHz microwave (MW) specific absorption rate (SAR, 3 W/kg) on human lymphocytes DNA damage induced by 4 chemical mutagens [mitomycin C (MMC), bleomycin (BLM), methyl methanesulfonate (MMS), and 4-nitroquinoline 1-oxide (4NQO)].


METHODS
Comet assay in vitro was used to detect human lymphocyte DNA damage induced by 1.8 GHz MW, 4 chemical mutagens, and MW plus 4 chemicals 0 h and 21 h respectively after exposure. The time exposed to MW or mutagens was 2 h or 3 h respectively. The results were showed by tail length (TL) and tail moment (TM).


RESULTS
The difference of DNA damage between MW group and control group was not statistically significant (P > 0.05). DNA damages in MW plus MMC groups and MW plus 4NQO groups were significantly greater than those in the corresponding concentrations of MMC groups and 4NQO groups (P < 0.01 or P < 0.05). However, MW did not enhance DNA damage induced by MMS and BLM (P > 0.05).


CONCLUSIONS
Exposure to 1.8 GHz (SAR, 3 W/kg) microwave may not induce human lymphocyte DNA damage, but could enhance DNA damage induced by MMC and 4NQO.
Wang
Bao-hong
BH
The Institute of Occupational and Environmental Health, Zhejiang University, Hangzhou 310006, China.
Lu
De-qiang
DQ
Jin
Li-fen
LF
Zheng
Wei
W
Lou
Jian-lin
JL
Deng
Hong-ping
HP
Li
Qing-yu
QY
He
Ji-liang
JL
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000328:Adult; D001761:Bleomycin / Q000633:toxicity; D002478:Cells, Cultured; D020552:Comet Assay; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects*; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D008872:Microwaves / Q000009:adverse effects*; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*
D009153:Mutagens; D001761:Bleomycin; D016685:Mitomycin; D015112:4-Nitroquinoline-1-oxide; D004247:DNA; D008741:Methyl Methanesulfonate