[Effect of Panax notoginseng saponins on intercellular adhesion molecule-1 expression and neutrophil infiltration in cerebral infarction tissue of rats].
OBJECTIVE
To investigate the effect of Panax notoginseng saponins (Pns) on the expression of intercellular adhesion molecule-1 (ICAM-1) and brain neutrophil infiltration induced by transient focal cerberal ischemia and reperfusion in rats.


METHODS
Focal cerebral ischemia-reperfusion model in rats (male) were made by transient occlusion of the middle cerebral artery (MCA) for 2 h followed by 24 h reperfusion. Pns 25 and 50 mg/kg were administered ip at 2 h after reperfusion. At 24 h after reperfusion, neutrophil infiltration and the expression of intercellular adhesion molecule-1 (ICAM-1) were measured with myeloperoxidase (MPO) activity, and immunohistochemistry, respectively.


RESULTS
After ischemia reperfusion, the expression of ICAM-1 and MPO activity in the ischemic hemisphere cortex of MCA area and caudate putamen were markedly increased. Treatment with 50 mg/kg Pns inhibited neutrophil infiltration and the expression of ICAM-1.


CONCLUSIONS
Pns inhibited neutrophil infiltration and the expression of ICAM-1 following focal cerebral ischemia-reperfusion in rats, attenuated the ischemic brain inflammation.
He
Wei
W
Gannan Medical College, Ganzhou, Jiangxi.
Zhu
Zunping
Z
D000818:Animals; D002544:Cerebral Infarction / Q000378:metabolism / Q000473:pathology*; D036145:Ginsenosides / Q000008:administration & dosage / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D020556:Neutrophil Infiltration / Q000187:drug effects*; D005894:Panax / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*
D036145:Ginsenosides; D018799:Intercellular Adhesion Molecule-1; D009195:Peroxidase