Moderate effect of enamel matrix derivative (Emdogain Gel) on Porphyromonas gingivalis growth in vitro.
OBJECTIVE
The aim of this study was to analyse the antibacterial effects of Emdogain Gel or its constituents on the growth of the suspected periodontopathogen Porphyromonas gingivalis.


METHODS
The effects of the proteins of enamel matrix derivative (EMD), the commercial product Emdogain Gel or its vehicle propylene glycol alginate (PGA) (Straumann, Switzerland) on P. gingivalis growth were determined by two methods: broth dilution assay (BDA) and agar diffusion assay (ADA).


RESULTS
BDA-Emdogain Gel inhibited moderately the growth of P. gingivalis, whereas EMD showed no effect. The PGA vehicle inhibited the growth completely. ADA-Emdogain Gel resulted in some inhibition in growth but was not significantly different from control. EMD revealed no zone of inhibition. PGA demonstrated statistically significant zones of inhibition.


CONCLUSIONS
Emdogain Gel shows moderate antibacterial activities against P. gingivalis. These properties seem to be due to the PGA component of the gel preparation.
Walter
Clemens
C
Department of Periodontology, Charité, University Medicine, Augustenburger Platz 1, 13353 Berlin, Germany. clemens.walter@unibas.ch
Jawor
Przemyslaw
P
Bernimoulin
Jean-Pierre
JP
Hägewald
Stefan
S
D000464:Alginates / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial / Q000379:methods; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D007164:Immunosorbents / Q000494:pharmacology; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000254:growth & development
D000464:Alginates; D000900:Anti-Bacterial Agents; D003746:Dental Enamel Proteins; D007164:Immunosorbents; C109069:enamel matrix proteins; C038550:propylene glycol alginate ester