Allele-specific, bidirectional silencing of an alcohol dehydrogenase gene in different organs of interspecific diploid cotton hybrids.
Interspecific hybridization is a common and important process that generates phenotypic novelty and can lead to hybrid speciation as well as to changes in gene expression. Using two different interspecific cotton (Gossypium) diploid hybrids, we show organ-specific, bidirectional allelic silencing at a heterozygous Adh locus, with alternate alleles being silenced in leaves and many floral organs, respectively. These results show that developmental regulation of gene expression is changed immediately upon hybridization between diploid species, possibly due to epigenetic factors or regulatory mismatch.
Adams
Keith L
KL
UBC Botanical Garden, Centre for Plant Research and Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada. keitha@interchange.ubc.ca
Wendel
Jonathan F
JF
D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D035264:Flowers / Q000235:genetics; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D003368:Gossypium / Q000235:genetics*; D006579:Heterozygote; D006824:Hybridization, Genetic; D018515:Plant Leaves / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D000426:Alcohol Dehydrogenase