Development of a coral cDNA array to examine gene expression profiles in Montastraea faveolata exposed to environmental stress.
The development of a cDNA array of coral genes and its application to investigate changes in coral gene expression associated with stressful conditions is described. The array includes both well-characterized and previously unidentified coral genes from Acropora cervicornis and Montastraea faveolata. Corals were exposed to either natural or anthropogenic stressors to elicit the expression of stress genes for isolation and incorporation onto the array. A total of 32 genes involved in protein synthesis, apoptosis, cell signaling, metabolism, cellular defense and inflammation were included on the array. Labeled cDNA from coral (Montastraea faveolata) exposed to elevated seawater temperature, salinity and ultraviolet light was tested against the microarray to determine patterns of gene expression associated with each stressor. Carbonic anhydrase, thioredoxin, a urokinase plasminogen activator receptor (uPAR) and three ribosomal genes demonstrated differential expression across all replicates on the array and between replicate colonies. Specific gene expression patterns produced in response to different stressors demonstrate the potential for gene expression profiling in characterizing the coral stress response.
Edge
Sara E
SE
School of Biology, Georgia Institute of Technology, 310 Ferst Drive, Atlanta, GA 30332-0230, USA. gte405r@mail.gatech.edu
Morgan
Michael B
MB
Gleason
Daniel F
DF
Snell
Terry W
TW
D000818:Animals; D037421:Anthozoa / Q000235:genetics* / Q000378:metabolism*; D015152:Blotting, Northern; D020224:Expressed Sequence Tags; D005431:Florida; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*; D013696:Temperature; D043922:Toxicogenetics / Q000379:methods; D014466:Ultraviolet Rays
D012965:Sodium Chloride