title: [Laboratory diagnosis of mycobacterial infections].
abstract: Every 10 seconds, one person in the world dies of tuberculosis (TB). It is estimated that one third of the world's population is latently infected with Mycobacterium tuberculosis. The proportion of multidrug-resistant strains of M. tuberculosis is increasing at an alarming rate in some parts of the world linked in part with the human immunodeficiency virus epidemic. For these reasons, TB remains a major public health problem, both in less-developed countries and in many industrialized countries, with 8-10 million new cases and 2 million deaths yearly in the world. Clinical, radiological and histological signs are not specific for tuberculosis or for other mycobacterial infections and allow only a presumptive diagnosis. In the same way, the tuberculin skin test is useful if the reaction is strong or phlyctenular because this test depends on various factors as previous BCG vaccination, contact or primary infection and host immune responses. The diagnosis of mycobacterial infection is proved only when bacilli are present in biological samples. Nevertheless, only 50% of cases in adults and 30% in infants have a positive bacteriological result. It seems necessary to develop new methods for a rapid and efficient diagnosis to optimize the therapy and the control of the epidemic. Laboratory testing in the mycobacterium field is experiencing more changes today than ever before. Determining what assays will be most useful to the clinician is a challenge, and acceptance of the new technology is under discussion. Progress in future will be linked probably to the progress of the genomic area. However the incidence rate is higher in less-developed countries, it is also important to develop now techniques possible to use in these countries. This review focuses on the current state-of-the-art resources useful for accurate and rapid laboratory diagnosis of mycobacterial infections.
authors.affiliation: Unité de génétique des mycobactéries, Institut Pasteur, 75015 Paris, France. valerie.martinez@psl.ap-hop-paris.fr
authors.forename: V
authors.initials: V
authors.lastname: Martinez
authors.forename: B
authors.initials: B
authors.lastname: Gicquel
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods; D001483:Base Sequence / Q000235:genetics; D006801:Humans; D009164:Mycobacterium Infections / Q000175:diagnosis* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D014376:Tuberculosis / Q000175:diagnosis