Apoptosis in Vero cells infected with Akabane, Aino and Chuzan virus.
Akabane, Aino and Chuzan virus are arthropod-borne (arbo) viruses mainly associated with reproductive failures in cattle. We investigated apoptosis in Vero cells (C-1586) infected with Akabane, Aino and Chuzan virus. The fragmentation of chromosomal DNA was simultaneously detected with the progress of cytopathic effect from 48 hr to 72 hr post infection, depending on viruses. Although the treatment of cycloheximide blocked apoptosis in Vero cells infected with three viruses, actinomycin D did not prevent DNA oligomerization, thus indicating that de novo viral protein synthesis is critical for viral apoptosis. In addition, the activation of caspase-3 was also detected in Vero cells by indirect fluorescent assay. From the present results, it is of future interest whether apoptotic characteristics of these viruses are related to pathogenecity in vivo.
Lim
Seong In
SI
National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang, Korea.
Kweon
Chang Hee
CH
Yang
Dong Kun
DK
Tark
Dong Seob
DS
Kweon
Jun Hun
JH
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002043:Bunyaviridae / Q000502:physiology*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral / Q000502:physiology*; D053938:DNA Fragmentation / Q000502:physiology; D003609:Dactinomycin; D004789:Enzyme Activation; D018163:Orbivirus / Q000502:physiology*; D014709:Vero Cells
D003609:Dactinomycin; D053148:Caspase 3; D020169:Caspases