title: [p53 gene codon 72 polymorphism and susceptibility to keloid].
abstract: OBJECTIVE
  To investigate the relationship between p53 codon 72 polymorphism and susceptibility to keloid in a southern Chinese population.
  
  
  METHODS
  The p53 genotypes were determined by polymerase chain reaction-reverse dot blot (PCR-RDB) and DNA direct sequencing in 45 patients with keloid and 60 unrelated healthy controls.
  
  
  RESULTS
  The frequency of the p53 Pro allele among keloid patients was significantly higher than that among healthy controls (chi2 = 4.485, P = 0.034). The Pro/Arg and Arg/Arg genotype distribution among keloid patients was not significantly different from that among healthy controls (chi2 = 0.949, 1.346; P = 0.330, 0.246, respectively). However, the Pro/Pro genotype frequency among keloid patients was significantly higher than that among healthy controls (chi2 = 4.375, P = 0.036). The p53 Pro/Pro genotype significantly increased the risk for developing keloid, compared to the combination of Pro/Arg and Arg/Arg genotypes,with the odds ratio (OR) of 2.400 (95%CI: 1.048-5.498).
  
  
  CONCLUSIONS
  Determination of the p53 codon 72 genotype may be used as a stratification marker to predicate high-risk individuals for keloid.
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhuo
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Gao
authors.forename: Shen-qiu
authors.initials: SQ
authors.lastname: Luo
authors.forename: Wei-sen
authors.initials: WS
authors.lastname: Zeng
authors.forename: Zhi-qi
authors.initials: ZQ
authors.lastname: Hu
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
authors.forename: Yong-zhong
authors.initials: YZ
authors.lastname: Zhao
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002648:Child; D002681:China / Q000453:epidemiology; D003062:Codon; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007627:Keloid / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D055815:Young Adult
chemical_list: D003062:Codon; D016159:Tumor Suppressor Protein p53