title: A study of glutathione status in the blood and tissues of patients with breast cancer.
abstract: Glutathione plays an important role in the antioxidant system that is required for the maintenance of the redox status of the cell, defence against free radicals and detoxification of toxic compounds. Reduced glutathione (redGSH) can be converted to oxidized glutathione (GSSG) during oxidative stress. The ratio of redGSH/total glutathione can be regarded as an index of the redox status and a useful indicator of disease risks. We conducted experiments by the capillary zone electrophoresis method to investigate the alterations of the glutathione status in the blood and tissue samples from patients with breast cancer. The results showed that the levels of redGSH, GSSG, total glutathione and the ratio of redGSH/total glutathione were significantly decreased in the blood of the patients with breast cancer compared to those of the control subjects. The levels of various forms of glutathione were lower and more pronounced in stage III. In contrast, the levels of redGSH, GSSG, total glutathione and the redGSH/total glutathione ratio in breast cancer tissues were significantly increased relative to those of the adjacent cancer-free tissues, especially in stage II. We suggest that the high redGSH levels are associated with the enhancement of cell proliferation and resistance to apoptosis in the cancer cells, and the loss of the large amount of erythrocyte redGSH may be due to increased detoxification capacities and defence against oxidative stress. We propose that redGSH should be regarded as an important biochemical parameter for detecting breast malignancy.
authors.affiliation: Graduate Institute of Medicine, Kaohsiung Medical University, No. 100 Shi-Chuan 1st Road, Kaohsiung, Taiwan.
authors.forename: Chih-Ching
authors.initials: CC
authors.lastname: Yeh
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Hou
authors.forename: Szu-Hsien
authors.initials: SH
authors.lastname: Wu
authors.forename: Shih-Meng
authors.initials: SM
authors.lastname: Tsai
authors.forename: Shu-Kai
authors.initials: SK
authors.lastname: Lin
authors.forename: Linda Ann
authors.initials: LA
authors.lastname: Hou
authors.forename: Hsu
authors.initials: H
authors.lastname: Ma
authors.forename: Li-Yu
authors.initials: LY
authors.lastname: Tsai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood* / Q000737:chemistry / Q000175:diagnosis*; D005260:Female; D005978:Glutathione / Q000032:analysis / Q000097:blood*; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D012016:Reference Values
chemical_list: D014408:Biomarkers, Tumor; D005978:Glutathione