Morphological variations of Anopheles minimus A in Tak Province, Thailand.
Anopheles minimus Theobald is one of the major vectors of malaria throughout the Oriental Region, and it's complex consists of at least 2 sibling species (A and C) in Thailand. This study aimed to determine the morphological variations of wings of An. minimus A and to clarify the specific status of An. minimus in Ban Khun Huay, Ban Pa Dae, and Ban Tham Seau, Mae Sot district, Tak Province, Thailand. Anopheline larvae were collected from the fields between October 2002 and September 2003, allowed to emerge into adults in the laboratory and identified by morphological and molecular characterization. About 1,715 of female An. minimus A were separated into 8 groups based on their wing scale patterns. Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) assay (ribosomal DNA ITS2) confirmed the identification of An. minimus A in all 8 groups.
Jaichapor
Boonsong
B
Department of Entomology, US Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Kengluecha
Ampornpan
A
Rongnoparut
Pornpimol
P
Rueda
Leopoldo M
LM
Jones
James W
JW
Sithiprasasna
Ratana
R
D000818:Animals; D000852:Anopheles / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics; D017931:DNA Primers; D005260:Female; D007303:Insect Vectors / Q000033:anatomy & histology* / Q000235:genetics; D007814:Larva; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D013785:Thailand; D014921:Wings, Animal / Q000033:anatomy & histology
D017931:DNA Primers