Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) for application of ascorbyl palmitate.
The aim of this study was to improve the chemical stability of ascorbyl palmitate (AP) in a colloidal lipid carrier for its topical use. For this purpose, AP-loaded solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLC) and for comparison, a nanoemulsion (NE) were prepared employing the high pressure homogenization technique and stored at room temperature (RT), 4 degrees C and 40 degrees C. During 3 months, physical stability of these formulations compared to placebo formulations which were prepared by the same production method, was studied including recrystallization behaviour of the lipid with differential scanning calorimetry (DSC), particle size distribution and storage stability with photon correlation spectroscopy (PCS) and laser diffractometry (LD). After evaluating data indicating excellent physical stability, AP-loaded SLN, NLC and NE were incorporated into a hydrogel by the same production method as the next step. Degradation of AP by HPLC and physical stability in the same manner were investigated at the same storage temperatures during 3 months. As a result, AP was found most stable in both the NLC and SLN stored at 4 degrees C (p > 0.05) indicating the importance of storage temperature. Nondegraded AP content in NLC, SLN and NE was found to be 71.1% +/- 1.4, 67.6% +/- 2.9 and 55.2% +/- 0.3 after 3 months, respectively. Highest degradation was observed with NE at all the storage temperatures indicating even importance of the carrier structure.
Uner
M
M
Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul University, Beyazit, Istanbul, Turkey. melikeuner@yahoo.com
Wissing
S A
SA
Yener
G
G
Müller
R H
RH
D001205:Ascorbic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D002138:Calibration; D002152:Calorimetry, Differential Scanning; D055598:Chemical Phenomena; D002626:Chemistry, Pharmaceutical; D002627:Chemistry, Physical; D003460:Crystallization; D018360:Crystallography, X-Ray; D004337:Drug Carriers; D004355:Drug Stability; D004356:Drug Storage; D004563:Electrochemistry; D005079:Excipients; D020100:Hydrogels; D008081:Liposomes / Q000737:chemistry*; D008863:Microspheres; D010316:Particle Size; D013696:Temperature
D004337:Drug Carriers; D005079:Excipients; D020100:Hydrogels; D008081:Liposomes; D001205:Ascorbic Acid; C031226:6-O-palmitoylascorbic acid