title: [Depression of mPges expression by troglitazone in human renal glomerulus induced by high glucose and LPS].
abstract: OBJECTIVE
  To determine the influence of troglitanzone on the expression of mPGEs induced by high glucose or LPS in human glomeruli cultured in vitro.
  
  
  METHODS
  Human glomeruli were isolated and incubated by LPS or high glucose absence or presence with different concentrations of troglitazone. The concentration of mPGEs in the supernatant was measured by an enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  LPS (10 microg/ml) and 5% glucose could improve the concentration of mPGEs significantly in the supernatant by cultured human glomeruli, and troglitazone (10 - 20 nmol/L) could block its increase (P < 0.05).
  
  
  CONCLUSIONS
  High glucose and LPS can improve mPGEs expression in cultured human glomeruli, and troglitazone can block this effects. These data suggest that PPAR-gamma agonist troglitanzone may be relevant to the treatment of diabetic nephropathy and inflammation in glomerluli.
authors.affiliation: Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha 410011 , China.
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Ling
authors.forename: You-Ming
authors.initials: YM
authors.lastname: Peng
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Wang
authors.forename: Shao-Bing
authors.initials: SB
authors.lastname: Duan
authors.forename: Ying-Hong
authors.initials: YH
authors.lastname: Liu
authors.forename: Fu-You
authors.initials: FY
authors.lastname: Liu
mesh_terms: D001786:Blood Glucose / Q000378:metabolism*; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology*; D003864:Depression, Chemical; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D007678:Kidney Glomerulus / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D047495:PPAR gamma / Q000819:agonists; D000071876:Prostaglandin-E Synthases; D045162:Thiazolidinediones / Q000494:pharmacology*; D000077288:Troglitazone
chemical_list: D001786:Blood Glucose; D002839:Chromans; D008070:Lipopolysaccharides; D047495:PPAR gamma; D045162:Thiazolidinediones; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D000077288:Troglitazone