Localization of cathepsin K and tartrate-resistant acid phosphatase in synovium and cranial cruciate ligament in dogs with cruciate disease.
OBJECTIVE
To localize cathepsin K and tartrate-resistant acid phosphatase (TRAP) in synovium and cranial cruciate ligament (CCL) of dogs with cruciate disease.


METHODS
Dogs (n=15) with cruciate disease and ruptured CCL, and 12 dogs with intact CCL.


METHODS
Synovium and CCL were examined histologically and cells containing cathepsin K or TRAP were identified immunohistochemically and histochemically, respectively.


RESULTS
Increased cellular localization of cathepsin K and TRAP was detected in synovium and ruptured CCL in dogs with cruciate disease, when compared with tissues from dogs with intact CCL. Inflammation of synovium with TRAP+ macrophage-like cells was seen in 73% of dogs with CCL disease, but was not seen in dogs with intact CCL. The presence of cathepsin K and TRAP protein in synovium and CCL tissues was significantly correlated in dogs with CCL rupture.


CONCLUSIONS
Inflammation of the epiligament of ruptured CCL with cathepsin K+ and TRAP+ macrophage-like cells forms part of a similar, more generalized chronic inflammatory change within the periarticular tissues of the stifle of a large proportion of dogs with CCL rupture.


CONCLUSIONS
Production of matrix-degrading enzymes by the synovium may induce progressive pathologic rupture of the CCL. Therefore, these collagenolytic pathways may offer a novel target for medical therapy of joint inflammation in canine patients with cruciate disease.
Muir
Peter
P
Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA. muirp@svm.vetmed.wisc.edu
Schamberger
Gwenn M
GM
Manley
Paul A
PA
Hao
Zhengling
Z
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D016118:Anterior Cruciate Ligament / Q000201:enzymology* / Q000473:pathology; D000070598:Anterior Cruciate Ligament Injuries; D016022:Case-Control Studies; D056657:Cathepsin K; D002403:Cathepsins / Q000378:metabolism*; D004283:Dog Diseases / Q000201:enzymology* / Q000473:pathology; D004285:Dogs / Q000293:injuries*; D005260:Female; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D007592:Joint Diseases / Q000201:enzymology / Q000662:veterinary*; D012421:Rupture / Q000201:enzymology / Q000662:veterinary; D013583:Synovial Membrane / Q000201:enzymology*; D000071681:Tartrate-Resistant Acid Phosphatase
D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002403:Cathepsins; D056657:Cathepsin K