A novel insertion sequence element, IS Yen2, as an epidemiological marker for weakly pathogenic bioserotypes of Yersinia enterocolitica.
The structure and distribution of IS Yen2, a new Yersinia enterocolitica insertion sequence element, were investigated. ISYen2 is related to IS elements of the IS21 family and is present in two isoforms in Y. enterocolitica serotype O:3. Analysis of all copies of both isoforms, IS Yen2A and IS Yen2B, by PCR and sequencing indicated that they are not flanked by direct repeats which are typical of the IS21 family of repetitive elements. IS Yen2 is present in multiple copies in Y. enterocolitica O:3 and O:9 and in a single copy in Y. enterocolitica O:1 and O:2 serotypes. The probe for IS Yen2 efficiently detected all weakly pathogenic Y. enterocolitica bioserotypes investigated, whereas it did not hybridize with other strains. This indicates that IS Yen2 can serve as an additional tool for Y. enterocolitica differentiation and epidemiological studies. Distribution of the different groups of IS elements in two Y. enterocolitica pathotypes is in favor of the parallel evolution of American and European Y. enterocolitica strains.
Golubov
Andrey
A
Yersinia Group I, Max von Pettenkofer-Institute for Hygiene and Medical Microbiology, Pettenkofer Str. 9a, D-80336 Munich, Germany.
Gierczynski
Rafal
R
Heesemann
Jürgen
J
Rakin
Alexander
A
D000595:Amino Acid Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012703:Serotyping / Q000379:methods; D014774:Virulence / Q000235:genetics*; D015009:Yersinia Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D015008:Yersinia enterocolitica / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial