Raman spectroscopy for optical diagnosis in the larynx: preliminary findings.
OBJECTIVE
Raman spectroscopy (RS) provides information about molecular structure and is a potential tool for non-invasive tissue diagnosis. To determine if Raman spectra could be obtained rapidly from laryngeal tissue in vitro, and compare Raman spectra from normal, benign, and cancerous laryngeal tissue.


METHODS
Forty-seven laryngeal specimens were studied using RS with signal acquisition times (SAT) between 1 and 30 second(s). Multivariate analysis was used to determine the diagnostic ability of RS compared to standard histology (n = 18, 13, and 16 respectively for normal tissue, carcinoma, and squamous papilloma).


RESULTS
Good quality spectra were obtained with 5-second SAT. Spectral peak analysis showed prediction sensitivities of 89%, 69%, and 88%, and specificities of 86%, 94%, and 94% for normal tissue, carcinoma, and papilloma.


CONCLUSIONS
In the larynx, spectral differences appear to exist between normal tissue, carcinoma, and papilloma. The ability to obtain spectra rapidly supports potential for future in vivo studies.
Lau
David P
DP
Department of Otolaryngology, Singapore General Hospital, Singapore 169608, Singapore. gollpc@sgh.com.sg
Huang
Zhiwei
Z
Lui
Harvey
H
Anderson
Donald W
DW
Berean
Ken
K
Morrison
Murray D
MD
Shen
Liang
L
Zeng
Haishan
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D006801:Humans; D066298:In Vitro Techniques; D007822:Laryngeal Neoplasms / Q000175:diagnosis*; D008875:Middle Aged; D010212:Papilloma / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods*