[Genotyping of Candida albicans strains isolated from nosocomial fungemia patients by pulsed-field gel electrophoresis].
Candida albicans has the most important place among fungal agents causing systemic infections. Molecular epidemiologic methods, especially pulsed-field gel electrophoresis (PFGE) are used frequently for outbreak investigations and to reveal existing clones in hospital environment. In this study, from 2000 through 2003, 42 C. albicans strains, isolated from the patients with nosocomial fungemia, were genotyped by PFGE. The results revealed that 31 different karyotypes existed among the study population. In conclusion, these C. albicans strains which were isolated in our hospital during the study period were not clonally related, whereas they were most probably arising from endogenous sources. It might be emphasized that the combination of PFGE method with a secondary method would be very useful to improve its discriminatory power although PFGE alone gives highly reproducible results.
Saraçli
Mehmet Ali
MA
GATA Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim, Mikoloji Bilim Dali, Ankara.
Yildiran
Sinasi Taner
ST
Gönlüm
Ahmet
A
Doğanci
Levent
L
D002176:Candida albicans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology*; D003428:Cross Infection / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D016469:Fungemia / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D007621:Karyotyping / Q000379:methods; D010802:Phylogeny