[A new method for studying the lipid transport in vitro: the protein with molecular weight 45-kDa from the rat olfactory epithelium--specific carrier of phosphatidylinositol 3,4,5-triphosphate].
A new method for studying the lipid-protein interactions in vitro is developed. It enables the study of the transporting activity of a protein toward a lipid ligand, including the case with an unknown lipid type. The method can be considered as a variant of partition three-phase chromatography with two stationary (donor and acceptor) phases and one mobile phase. The protein under study is dissolved in an aqueous mobile phase and induces a specific delivery of a lipid to the acceptor lipid layer. The transported lipid is identified in the Folch lipid extracts from the acceptor layer and aqueous phase. The secretory protein with M 45 kDa from the rat olfactory epithelium is shown to be a carrier of phosphatidylinositol 3,4,5-triphosphate. Our approach opens up new possibilities in the study of lipid-protein interactions in vitro and has a number of advantages over the methods now used for these purposes.
Riadchenko
V V
VV
Shuvaeva
T M
TM
Il'nitskaia
E V
EV
Tret'iakov
V E
VE
Lipkin
V M
VM
D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry*; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry*; D008954:Models, Biological; D008970:Molecular Weight; D009831:Olfactory Mucosa / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000737:chemistry*; D051381:Rats
D002352:Carrier Proteins; D008024:Ligands; D008051:Lipid Bilayers; D018129:Phosphatidylinositol Phosphates; C060974:phosphatidylinositol 3,4,5-triphosphate