Evidence of the protective immunogenicity of native and recombinant Clostridium haemolyticum phospholipase C (beta toxin) in guinea pigs.
The immunogenic effects of the beta toxin of Clostridium haemolyticum were investigated in guinea pigs. Guinea pigs passively immunized with toxin-neutralizing monoclonal antibodies were protected from a 100 LD(50) spore challenge that was lethal to nonvaccinated controls. Guinea pigs actively immunized with varying doses of immunoaffinity-purified native beta toxin were similarly protected. In a third experiment, a recombinant toxoid was prepared from E. coli expressing the beta toxin gene. Guinea pigs immunized three times with recombinant toxoid also were protected against challenge. In each experiment, protection was correlated to the presence of anti-beta toxin antibodies in the serum. Taken together, these results indicate that a neutralizing antibody response to the beta toxin is a key component of protective immunity to C. haemolyticum in guinea pigs.
Hauer
Paul J
PJ
Center for Veterinary Biologics, Veterinary Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Ames, IA 50010, USA. Paul.J.Hauer@aphis.usda.gov
Yeary
Teresa J
TJ
Rosenbusch
Ricardo F
RF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D001483:Base Sequence; D002846:Chromatography, Affinity / Q000379:methods; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology*; D017931:DNA Primers; D005260:Female; D006168:Guinea Pigs; D007106:Immune Sera; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D054799:Phospholipase C beta; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D010738:Type C Phospholipases / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D017931:DNA Primers; D007106:Immune Sera; D007527:Isoenzymes; D011994:Recombinant Proteins; D010738:Type C Phospholipases; D054799:Phospholipase C beta