Amygdalin inhibits genes related to cell cycle in SNU-C4 human colon cancer cells.
OBJECTIVE
The genes were divided into seven categories according to biological function; apoptosis-related, immune response-related, signal transduction-related, cell cycle-related, cell growth-related, stress response-related and transcription-related genes.


METHODS
We compared the gene expression profiles of SNU-C4 cells between amygdalin-treated (5 mg/mL, 24 h) and non-treated groups using cDNA microarray analysis. We selected genes downregulated in cDNA microarray and investigated mRNA levels of the genes by RT-PCR.


RESULTS
Microarray showed that amygdalin downregulated especially genes belonging to cell cycle category: exonuclease 1 (EXO1), ATP-binding cassette, sub-family F, member 2 (ABCF2), MRE11 meiotic recombination 11 homolog A (MRE11A), topoisomerase (DNA) I (TOP1), and FK506 binding protein 12-rapamycin-associated protein 1 (FRAP1). RT-PCR analysis revealed that mRNA levels of these genes were also decreased by amygdalin treatment in SNU-C4 human colon cancer cells.


CONCLUSIONS
These results suggest that amygdalin have an anticancer effect via downregulation of cell cycle-related genes in SNU-C4 human colon cancer cells, and might be used for therapeutic anticancer drug.
Park
Hae-Jeong
HJ
Department of Pharmacology, Kohwang Medical Research Institute, College of Pharmacy, Kyung Hee University, Seoul 130-701, South Korea.
Yoon
Seo-Hyun
SH
Han
Long-Shan
LS
Zheng
Long-Tai
LT
Jung
Kyung-Hee
KH
Uhm
Yoon-Kyung
YK
Lee
Je-Hyun
JH
Jeong
Ji-Seon
JS
Joo
Woo-Sang
WS
Yim
Sung-Vin
SV
Chung
Joo-Ho
JH
Hong
Seon-Pyo
SP
D000678:Amygdalin / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle / Q000235:genetics*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D015536:Down-Regulation; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans
D000972:Antineoplastic Agents, Phytogenic; D000678:Amygdalin