title: Identification of Candida strains isolated from Tanzanian pregnant women with vaginal candidiasis.
abstract: OBJECTIVE
  To identify Candida strains isolated from Tanzanian women (13 to 45 years) with vaginal candidiasis.
  
  
  METHODS
  A cross-sectional study.
  
  
  METHODS
  Antenatal clinic in Ilala district hospital in Dar es Salaam, Tanzania from March 1998 to December 2000.
  
  
  RESULTS
  The identities of the 272 isolates tested with API Candida were: Candida albicans 180(66.2%), Candida tropicalis 13(4.7%), Candida glabrata 20(7.35%), Candida famata 6(2.2%), Candida parapsilosis 6 (2.2%) and Candida lusitaniae one (0.37%). API Candida could not speciate 43 (15.8%) isolates of these; two (0.7%) fell between C. albicans and C. tropicalis, 17(6.25%) C. lusitaniael, C. guilliermondii/C. famata, 14(5.15%) C. krusei, C. inconspicua, and C. norvegensis and nine (3.3%) either C. parapsilosis, C. krusei, C. incospicua or Geotrichum spp. Four (1.5%) isolates had an assimilation pattern of Trichosporo spp, but were all germ tube positive and had morphological features on cornemeal agar that were consistent with C. albicans. API 20C AUX was used for testing 29 isolates and results showed: 11/29 (37.9%) C. albicans, 1/29 (3.4%) C. tropicalis, 4/29 (13.8%) C. glabrata, 1/29 (3.4%) C. parapsilosis, 1/29 (3.4%) C. famata, 1/29 (3.4%) C. lusitaniae, 1/29 (3.4%) C. colliculosa/C. magnoliae, 5/29(17.2%) C. albicans/ C. tropicals 2/29 (6.8%) C. norvegensis/C. parapsilosis, and 2/29(6.8%) C. kruseil/C. inconspicua. Results of 20 isolates identified by Randomly Amplified Polymorphic DNA (RAPID) technique showed a 95% agreement with API Candida and a 100% agreement with API 20C AUX.
  
  
  CONCLUSIONS
  Although most (66.3%) of the species isolated from Dar es Salaam women with vaginal candidiasis were C. albicans, a considerable percentage (33.7%) were non-albicans, mainly C. glabrata, C. krusei and C. tropicalis. The high prevalence of non-albicans Candida spp observed in this population may have therapeutic implications.
authors.affiliation: Applied Microbiology Unit, Department of Botany, Faculty of Medicine, Muhimbili University College of Health Sciences, Dar es Salaam, Tanzania.
authors.forename: L A
authors.initials: LA
authors.lastname: Namkinga
authors.forename: M I N
authors.initials: MI
authors.lastname: Matee
authors.forename: A K
authors.initials: AK
authors.lastname: Kivaisi
authors.forename: A
authors.initials: A
authors.lastname: Kullaya
authors.forename: E E
authors.initials: EE
authors.lastname: Mneney
mesh_terms: D000293:Adolescent; D000328:Adult; D002175:Candida / Q000302:isolation & purification*; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D013636:Tanzania