title: Single-walled carbon nanotube biosensors using aptamers as molecular recognition elements.
abstract: We report the real-time detection of protein using SWNT-FET-based biosensors comprising DNA aptamers as molecular recognition elements. Anti-thrombin aptamers that are highly specific to serine protein thrombin were immobilized on the sidewall of a SWNT-FET using CDI-Tween linking molecules. The binding of thrombin aptamers to SWNT-FETs causes a rightward shift of the threshold gate voltages, presumably due to the negatively charged backbone of the DNA aptamers. While the addition of thrombin solution causes an abrupt decrease in the conductance of the thrombin aptamer immobilized SWNT-FET, no noticeable change was observed with elastase.
authors.affiliation: Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 305-343, Korea.
authors.forename: Hye-Mi
authors.initials: HM
authors.lastname: So
authors.forename: Keehoon
authors.initials: K
authors.lastname: Won
authors.forename: Yong Hwan
authors.initials: YH
authors.lastname: Kim
authors.forename: Byoung-Kye
authors.initials: BK
authors.lastname: Kim
authors.forename: Beyong Hwan
authors.initials: BH
authors.lastname: Ryu
authors.forename: Pil Sun
authors.initials: PS
authors.lastname: Na
authors.forename: Hyojin
authors.initials: H
authors.lastname: Kim
authors.forename: Jeong-O
authors.initials: JO
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013917:Thrombin / Q000737:chemistry*; D013997:Time Factors
chemical_list: D037742:Nanotubes, Carbon; D009841:Oligonucleotides; D004247:DNA; D013917:Thrombin