Expression of calcium-binding proteins in the proliferative zones around the corticostriatal junction of rabbits during pre- and postnatal development.
Herein we asked whether cells expressing calcium-binding proteins around the corticostriatal junction are of pallial or subpallial origin. Brains of rabbit embryos between embryonic day E18 and E28 and postnatal day 0-P22 were immunoreacted with monoclonal antibodies raised against calretinin, calbindin and parvalbumin. At E18-E21, calbindin- and calretinin-immunoreactive cells were seen in distinct proliferative zones in the vicinity of the corticostriatal junction. Whereas calbindin-immunoreactive neurons were in the ventricular zone of the ventral pallium (the medial wall of the lateral ventricular angle), calretinin-immunoreactive cells were, nearby, in the subventricular zone of the subpallium at the lateral edge of the lateral ganglionic eminence. From E25 to P22, both calbindin- and calretinin-immunoreactive cells appeared in the pallial ventricular and subventricular zones around the lateral ventricular angle. Some of these cells resembled migratory neuroblasts. Parvalbumin-immunoreactive cells appeared at P5-P10, albeit they were almost negligible in the proliferative zones around the corticostriatal junction and the lateral ventricular angle. The results suggest that a number of the calbindin-expressing neurons that are generated in mid-gestation and postnatally are of pallial origin. They also indicate that only a few of the late-generated calretinin-immunoreactive cells may have a pallial source. The origin of the parvalbumin-immunoreactive cells was not ascertained in the present study.
Reblet
C
C
Department of Neurosciences, School of Medicine and Dentistry, The University of the Basque Country, Leioa, Spain. onpreloc@lg.chu.es
Alejo
A
A
Fuentes
T
T
Pró-Sistiaga
P
P
Mendizabal-Zubiaga
J
J
Bueno-López
J L
JL
D000818:Animals; D000831:Animals, Newborn; D064032:Calbindin 2; D064026:Calbindins; D002135:Calcium-Binding Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002540:Cerebral Cortex / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D003342:Corpus Striatum / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D010320:Parvalbumins / Q000378:metabolism; D011817:Rabbits; D064030:S100 Calcium Binding Protein G / Q000378:metabolism
D064032:Calbindin 2; D064026:Calbindins; D002135:Calcium-Binding Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G