Expression of myristoyltransferase and its interacting proteins in epilepsy.
N-Myristoylation is a co-translational, irreversible addition of a fatty acyl moiety to the amino terminus of many eukaryotic cellular proteins. This modification is catalyzed by N-myristoyltransferase (NMT) and is recognized to be a widespread and functionally important modification of proteins. The myristoylated Src family kinases are involved in various signaling cascades, including the N-methyl-d-aspartate receptor functions. We examined the expression of NMT and its interacting proteins to gain further insight into the mechanisms in epileptic fowl. Higher expression of NMT1 and NMT2 was observed in carrier and epileptic fowl whereas expression of heat shock cognate protein 70, an inhibitor of NMT, was lower. Furthermore, protein-protein interaction of NMT with m-calpain, caspase-3, and p53 was established. The interaction of NMT2 with caspase-3 and p53 was weak in epileptic fowl compared with normal chicks while the interaction of NMT1 with m-calpain was weak in epileptics. Understanding the regulation of NMT by specific inhibitors may help us to control the action of this enzyme on its specific substrates and may lead to improvements in the management of various neurological disorders like Alzheimer's disease, ischemia, and epilepsy.
Selvakumar
Ponniah
P
Department of Pathology, College of Medicine and Health Research Division, Saskatchewan Cancer Agency, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 4H4.
Lakshmikuttyamma
Ashakumary
A
Charavaryamath
Chandrashekhar
C
Singh
Baljit
B
Tuchek
John
J
Sharma
Rajendra K
RK
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D004827:Epilepsy / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping
D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C492718:N-myristoyltransferase inhibitor protein 71, human; D009363:Neoplasm Proteins; D000217:Acyltransferases; C050480:glycylpeptide N-tetradecanoyltransferase