title: Transient ischemia-induced changes of neurofilament 200 kDa immunoreactivity and protein content in the main olfactory bulb in gerbils.
abstract: This study was carried out to investigate alterations of neurofilament 200 kDa (NF-200) and its polyphosphorylation form (RT97) immunoreactivity and protein content in the main olfactory bulb (MOB) after 5 min of transient forebrain ischemia in gerbils. In the sham-operated group, weak NF-200 immunoreactivity was detectable in a few somata of mitral cells, which projected weak NF-200-immunoreactive processes to the external plexiform layer (EPL). At 1-5 days after ischemia, strong NF-200 and RT97 immunoreactivity was shown by the mitral cell processes; however, somata of mitral cells did not show NF-200 immunoreactivity. At this time point, strong NF-200-immunoreactive mitral cell processes ran to the EPL and glomerular layer (GL). Thereafter, NF-200 and RT97 immunoreactivity was decreased up to 30 days after ischemia. In the 15 days post-ischemic group, the distribution pattern of NF-200 and RT97 immunoreactivity was slightly lower than that in the 1-5 days post-ischemic groups. In the 30 days post-ischemic group, moderate NF-200 and RT97 immunoreactivity was found in the mitral cells processes, but the immunoreactivity in the EPL and GL nearly disappeared. A Western blot study showed a pattern of NF-200 and RT97 expression at all post-ischemic time points similar to that of immunohistochemistry after ischemia. This result indicates that NF-200 and RT97 accumulates in injured mitral cell processes a few days after transient ischemia, which suggests that the axonal transport in the MOB may be disturbed during this period after transient ischemia.
authors.affiliation: Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, South Korea.
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Un-San
authors.initials: US
authors.lastname: Koh
authors.forename: Jae Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Song
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Jung
authors.forename: Young Sam
authors.initials: YS
authors.lastname: Nam
authors.forename: In Se
authors.initials: IS
authors.lastname: Lee
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D001369:Axons / Q000276:immunology / Q000473:pathology; D015153:Blotting, Western; D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D003599:Cytoskeleton / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005849:Gerbillinae; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D016900:Neurofilament Proteins / Q000378:metabolism*; D009474:Neurons / Q000276:immunology / Q000473:pathology; D019581:Neuropil / Q000276:immunology / Q000473:pathology; D009830:Olfactory Bulb / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009833:Olfactory Pathways / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D010766:Phosphorylation; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D013997:Time Factors
chemical_list: D016900:Neurofilament Proteins; D011506:Proteins; C051774:neurofilament protein H