title: Validation of liquid chromatographic method for analysis of lidocaine hydrochloride, dexamethasone acetate, calcium dobesilate, buthylhydroxyanisol and degradation product hydroquinone in suppositories and ointment.
abstract: In this paper, there was developed a sensitive, precise and accurate reversed-phase liquid chromatographic (RP-HPLC) method and validated for simultaneous determination of lidocaine hydrochloride, dexamethasone acetate (DA) and calcium dobesilate (CD) in suppositories and ointment. Also there was achieved a parallel analysis of buthylhydroxyanisol, as a preservative, and hydroquinone, as a degradation product of calcium dobesilate, present in these dosage forms. The relative standard deviation (RSD) values for all five compounds indicated a good precision and accuracy of the RP-HPLC method. Method is selective, sensitive and reproducible with good recovery values and can be applied in simultaneous determination of all mentioned compounds.
authors.affiliation: Department of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, Vojvode Stepe 450, Belgrade, Serbia. ljzivan@pharmacy.bg.ac.yu
authors.forename: Lj
authors.initials: Lj
authors.lastname: Zivanovic
authors.forename: M
authors.initials: M
authors.lastname: Zecevic
authors.forename: S
authors.initials: S
authors.lastname: Markovic
authors.forename: S
authors.initials: S
authors.lastname: Petrovic
authors.forename: I
authors.initials: I
authors.lastname: Ivanovic
mesh_terms: D002083:Butylated Hydroxyanisole / Q000032:analysis*; D002123:Calcium Dobesilate / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000032:analysis; D006873:Hydroquinones / Q000032:analysis*; D008012:Lidocaine / Q000032:analysis*; D009824:Ointments / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013488:Suppositories / Q000737:chemistry*
chemical_list: D006873:Hydroquinones; D009824:Ointments; D013488:Suppositories; D002083:Butylated Hydroxyanisole; D002123:Calcium Dobesilate; D003907:Dexamethasone; D008012:Lidocaine; C018038:dexamethasone acetate; C031927:hydroquinone