title: Changes in gelatinase activity in the gastrointestinal tract after anastomotic construction in the ileum or colon.
abstract: OBJECTIVE
  The strength of the uninjured and anastomosed intestinal wall is determined by its submucosal connective tissue. Matrix degradation by matrix metalloproteinases may result in loss of strength. It is known that anastomotic construction leads to up-regulation of matrix metalloproteinase activity in the wound area, but no quantitative data are available as to the extent of this effect throughout the intestinal wall. This study was designed to quantitate changes in gelatinolytic activity in the intestine after anastomotic construction in the ileum or colon.
  
  
  METHODS
  An anastomosis was constructed in the distal ileum or distal colon of rats, and animals were killed after one or three days. Tissue samples (5 mm) were collected containing the suture line, its adjacent segments (2- x 5-mm in both directions) and at nine other, more distant, sites throughout the gastrointestinal tract. Similar samples were collected from nonoperated control rats. All samples were analyzed by quantitative gelatin zymography.
  
  
  RESULTS
  In control rats, the most prominent gelatinolytic activities were found at 80 kDa, thought to represent a nonspecific proteolytic activity, 60 kDa and 50 kDa, representing the proform and active form of matrix metalloproteinase-2, respectively. Activities were higher in the small bowel than in the large bowel. Anastomotic construction led to massive up-regulation of an activity at 105 kDa, and its dimer, believed to represent promatrix metalloproteinase-9. Matrix metalloproteinase-2 remained unaffected, whereas the activity of the 80 kDa protein was significantly (P < 0.05) reduced. Significantly increased matrix metalloproteinase-9 activity was found in the actual anastomotic segments and in the immediately adjacent tissue. Matrix metalloproteinase-9 activities in the anastomotic segments were highest at Day 1 in the ileum and at Day 3 in the colon. Anastomotic construction in the ileum or colon did not lead to any significant changes of any gelatinolytic activity at the more distant sites in the bowel wall.
  
  
  CONCLUSIONS
  Up-regulation of gelatinase activity after anastomotic construction in the intestine is caused by matrix metalloproteinase-9. Because the effect is local and not systemic, unwanted matrix degradation at distant sites seems unlikely.
authors.affiliation: Department of Surgery, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
authors.forename: Ignace H J T
authors.initials: IH
authors.lastname: de Hingh
authors.forename: Roger M L M
authors.initials: RM
authors.lastname: Lomme
authors.forename: Harry
authors.initials: H
authors.lastname: van Goor
authors.forename: Robert P
authors.initials: RP
authors.lastname: Bleichrodt
authors.forename: Thijs
authors.initials: T
authors.lastname: Hendriks
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000601:surgery*; D018093:Gelatinases / Q000378:metabolism*; D007082:Ileum / Q000601:surgery*; D007422:Intestines / Q000201:enzymology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000201:enzymology*; D013997:Time Factors
chemical_list: D018093:Gelatinases