title: [Construction of cell lines producing high-titer recombinant retroviruses of message and antisense cDNA of human glucocorticoid receptor beta].
abstract: OBJECTIVE
  To construct cell lines producing high-titer recombinant retroviruses of human glucocorticoid receptor beta (hGRbeta).
  
  
  METHODS
  The recombinant retrorinal vectors of message and antisense cDNA of hGRbeta were transfected into PA317 cells by liposomes, respectively, and cell colonies were selected with G418. The titers of viruses from the 2 cell culture supernatants were determined by colony numbers produced in NIH3T3 cells.
  
  
  RESULTS
  The presence of aimed RNAs in supernatant of the G418-resistant cell cultures were detected by RT-PCR and subsequent sequence analysis. The titers of retroviruses were 2. 0 x 10(5) and 1.8 x 10(5), each for recombinant message and antisense hGRbeta cDNA.
  
  
  CONCLUSIONS
  We demonstrated here the obtaining of transfect PA317 cells that produce recombinant retroviruses containing message and antisense RNAs of hGRbeta with highly infective efficiencies.
authors.affiliation: Molecular Biology Research Center, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Qing-nan
authors.initials: QN
authors.lastname: He
authors.forename: Gang
authors.initials: G
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: C I
authors.initials: CI
authors.lastname: Msangi
authors.forename: Wei-xin
authors.initials: WX
authors.lastname: Hu
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D016373:DNA, Antisense / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection
chemical_list: D016373:DNA, Antisense; D018076:DNA, Complementary; D011965:Receptors, Glucocorticoid; D011994:Recombinant Proteins; C121943:glucocorticoid receptor beta