Membrane on a chip: a functional tethered lipid bilayer membrane on silicon oxide surfaces.
Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as functionality of a bilayer membrane. Here, we describe the synthesis of lipids for a more modular design and the adaptation of the linker part to silane chemistry. We were able to form a functional tethered bilayer lipid membrane with good electrical sealing properties covering a silicon oxide surface. We demonstrate the functional incorporation of the ion carrier valinomycin and of the ion channel gramicidin.
Atanasov
Vladimir
V
Max Planck Institute for Polymer Research, 55128 Mainz, Germany.
Knorr
Nikolaus
N
Duran
Randolph S
RS
Ingebrandt
Sven
S
Offenhäusser
Andreas
A
Knoll
Wolfgang
W
Köper
Ingo
I
D001703:Biophysics / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry; D006096:Gramicidin / Q000494:pharmacology; D007473:Ion Channels / Q000737:chemistry*; D007476:Ionophores / Q000494:pharmacology; D007477:Ions; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D010713:Phosphatidylcholines / Q000737:chemistry; D010836:Phytol / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D012821:Silanes / Q000737:chemistry* / Q000378:metabolism; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013053:Spectrophotometry; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors; D014634:Valinomycin / Q000737:chemistry / Q000494:pharmacology
C506632:2,3-di-O-phytanyl-sn-glycerol-1-tetraethylene glycol-(3-chloro-dimethylpropyl-silane) ether lipid; C506630:2,3-di-O-phytanyl-sn-glycerol-1-tetraethylene glycol-(3-trichloropropyl-silane) ether lipid; D007473:Ion Channels; D007476:Ionophores; D007477:Ions; D008051:Lipid Bilayers; D008055:Lipids; D010713:Phosphatidylcholines; D012821:Silanes; D006096:Gramicidin; D010836:Phytol; D014634:Valinomycin; C021016:1,2-diphytanoylphosphatidylcholine; D006046:Gold; D012822:Silicon Dioxide; D012825:Silicon