The mitochondrial genome of Debaryomyces (Schwanniomyces) occidentalis encodes subunits of NADH dehydrogenase complex I.
nad genes encoding subunits of the NADH dehydrogenase complex 1 have been revealed in the yeast Debaryomyces (Schwanniomyces) occidentalis. nad1, nad3, nad5, nad6 and most large mitochondrial genes have been located on a circular 41-kb map of mitochondrial DNA from this petite negative species. The genes nad1-nad6 are co-transcribed and the transcription is not inhibited by glucose. Sequences of nad6 and 5'-nad1 compared to homologs in other yeasts indicate better amino acids conservation for nad1 product than for nad6. A cytochrome b deficient mutant dependent on alternative oxidase and functional complex 1 for growth on respirable substrates also exhibits co-transcription of nad1-nad6.
Fernet
Caroline
C
Centre de Génétique Moléculaire, UPR 2167 du CNRS associée a l'UPMC, Paris 6, Gif-sur-Yvette, 91198, France. fernet@cgm.cnrs-gif.fr
Claisse
Maurice
M
Clark-Walker
G Desmond
GD