title: Extremely low electrical current generated by porcine small intestine smooth muscle alters bacterial autolysin production.
abstract: The effect of extremely low electrical currents, identical to those generated by the gut smooth muscle, on bacterial autolysin production in vitro was tested in the present study. When stimulated with these electrical currents, the bacteria Pediococcus pentosaceus 16:1 produced groups of peptidoglycan hydrolases that differed from those produced by the unstimulated (control) bacteria. The autolysins synthesized by the P. pentosaceus 16:1 under extremely low electrical currents were effective against peptidoglycans from the cell walls of various lactic acid bacteria strains, whereas the autolysins from the control bacteria acted exclusively against P. pentosaceus 16:1 cell walls. Thus, it can be predicted that in vivo the electrical currents generated by the intestinal smooth muscles, which can be recorded as the myoelectrical migrating complexes, could regulate lactic acid bacteria strain growth in the gut.
authors.affiliation: Department of Laboratory Medicine, Lund University, Sölvegatan 23, SE-223 62 Lund, Sweden.
authors.forename: Danuta
authors.initials: D
authors.lastname: Kruszewska
authors.forename: Pawel
authors.initials: P
authors.lastname: Podgurniak
authors.forename: Asa
authors.initials: A
authors.lastname: Ljungh
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Sebastian
authors.forename: Lennart
authors.initials: L
authors.lastname: Larsson
authors.forename: Jolanta
authors.initials: J
authors.lastname: Zajdel-Dabrowska
authors.forename: Stefan G
authors.initials: SG
authors.lastname: Pierzynowski
mesh_terms: D000818:Animals; D002473:Cell Wall / Q000201:enzymology; D015180:Electrophoresis, Gel, Two-Dimensional; D004594:Electrophysiology; D007421:Intestine, Small / Q000382:microbiology* / Q000502:physiology; D007778:Lactobacillus / Q000502:physiology; D007934:Leuconostoc / Q000502:physiology; D009130:Muscle, Smooth / Q000502:physiology*; D016065:Myoelectric Complex, Migrating / Q000502:physiology*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000096:biosynthesis*; D010376:Pediococcus / Q000502:physiology*; D010457:Peptidoglycan / Q000378:metabolism; D013552:Swine
chemical_list: D010457:Peptidoglycan; D009238:N-Acetylmuramoyl-L-alanine Amidase