title: Amblyomma tigrinum (Acari: Ixodidae): new data on hosts and biology of immature stages and on DNA composition.
abstract: Biological data of immature stages of Amblyomma tigrinum were obtained from larvae and nymphs both fed on rats and rabbits. Data from nymphs recovered from a wild rodent (Galea musteloides) are also reported. Additional results in DNA composition of males moulted from nymphs fed on laboratory and wild hosts are presented. The ticks were maintained in darkness at 27 +/- 1 degrees C and 83-86% RH. The mean recovery rates were 49.1% and 43.6% with a moulting success of 96.2% and 90.8% in larvae fed on rats and rabbits, respectively. The engorgement weights were almost identical in larvae recovered from both hosts. The mean recovery rates of nymphs were 37.3% and 69.9% in specimens fed on rats and rabbits, respectively. The moulting success was 94.5%, 100% and 98.1% in nymphs fed on rats, rabbits and G. musteloides, respectively. Nymphs from all hosts moulting to females were significantly heavier (P < 0.01) than those moulting to males despite their range of engorgement weight showed overlap. A higher proportion (>or=61.5%) of nymphs from all hosts moulted to females. Present results suggest that members of Rodentia and Lagomorpha are suitable hosts for the immature stages of A. tigrinum, contrasting with previous results from Brazilian colonies of this tick. The DNA sequence from ticks fed on G. musteloides showed 99.7% identity with that from ticks fed on rabbits and also with the DNA sequence already available (GenBank AY498562 ) for A. tigrinum.
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Salta, CC 228, CP 4400 Salta, Argentina. daguirre@correo.inta.gov.ar
authors.forename: D H
authors.initials: DH
authors.lastname: Aguirre
authors.forename: A J
authors.initials: AJ
authors.lastname: Mangold
authors.forename: M M
authors.initials: MM
authors.lastname: Cafrune
authors.forename: A A
authors.initials: AA
authors.lastname: Guglielmone
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D005260:Female; D026863:Ixodidae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D008297:Male; D018978:Molting / Q000502:physiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S