Negative regulation of DNA repair gene (uvrA) expression by ArcA/ArcB two-component system in Escherichia coli.
The ArcA/ArcB two-component signal transduction system of Escherichia coli regulates gene expression in response to the redox conditions of growth. In this study, uvrA gene expression was repressed when ArcA was induced in E. coli. Transcription of uvrA increased in DeltaarcA and DeltaarcB strains more than in the wild-type strain, whose trend was remarkable under the anaerobic condition. In the wild-type strain grown in the presence of DTT (10 mM), the uvrA gene expression was also repressed. Furthermore, the results of in vitro transcription and DNase I footprinting experiments indicated that ArcA specifically bound to the ArcA box [(A/T)GTTAATTA(A/T)] in the uvrA promoter and represses its transcription. These results suggest that the ArcA/ArcB two-component system works to negatively regulate uvrA gene expression.
Ogasawara
Hiroshi
H
Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan.
Teramoto
Jun
J
Yamamoto
Satoshi
S
Hirao
Kiyo
K
Yamamoto
Kaneyoshi
K
Ishihama
Akira
A
Utsumi
Ryutaro
R
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D018983:DNA Footprinting; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008565:Membrane Proteins / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D015398:Signal Transduction
D001425:Bacterial Outer Membrane Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D012097:Repressor Proteins; C041610:arcA protein, E coli; D011494:Protein Kinases; C065271:arcB protein, E coli; C068631:UvrA protein, E coli; D000251:Adenosine Triphosphatases