title: Identification of platelet receptors for the Streptococcus gordonii DL1 sialic acid-binding adhesin.
abstract: Aggregation of human platelets by Streptococcus gordonii DL1, an interaction implicated in the pathogenesis of infective endocarditis, requires the expression of hsa, the gene encoding the sialic acid-binding adhesin (Hsa) of this organism. To identify the sialoglycoproteins on the platelet surface as the receptors for Hsa, intrinsic membrane proteins were assessed by bacterial overlay assay. S. gordonii DL1 adhered to 130-140-kDa proteins, a reaction that was abolished by neuraminidase treatment of immobilized platelet surface proteins. These sialoglycoproteins were identified as platelet glycoprotein Ib alpha (GPIbalpha ) and glycoprotein IIb (GPIIb) by immunoprecipitation with specific monoclonal antibody against each glycoprotein.
authors.affiliation: Department of Microbiology, Nippon Dental University School of Dentistry at Tokyo, Tokyo, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Yajima
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Konishi
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000502:physiology*; D001792:Blood Platelets / Q000737:chemistry*; D002352:Carrier Proteins / Q000378:metabolism*; D006389:Hemagglutinins, Viral; D006801:Humans; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D013291:Streptococcus / Q000737:chemistry*
chemical_list: D018829:Adhesins, Bacterial; D002352:Carrier Proteins; D006389:Hemagglutinins, Viral; D019038:Platelet Glycoprotein GPIb-IX Complex; D010980:Platelet Membrane Glycoproteins; C023592:glycocalicin; C032193:glycoprotein receptor GPIb-IX; C450840:hsa protein, Streptococcus gordonii