[Influence of carbohydrate substrate feeding rate on productivity of recombinant Escherichia coli strain].
Effect of different regimes of glucose feeding on growth rate and recombinant protein synthesis of recombinant E. coli was investigated. It was estimated that the selected regime of substrate feeding provided enhanced finished product yield up to 40-50 per cent. Changes in feeding regime caused significant changes in the quantity and pattern of the glucose metabolism by- products.
Chezhgalova
N A
NA
Ustiuzhanina
S V
SV
Iarotskiĭ
S V
SV
D001709:Biotechnology / Q000379:methods; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D011384:Proinsulin / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis*
D002241:Carbohydrates; D011994:Recombinant Proteins; D011384:Proinsulin; D005947:Glucose