Immunosorbent assay microchip system for analysis of human immunoglobulin G on MagnaBind carboxyl derivatized beads.
An immunosorbent assay system was integrated into a PMVS microchip. MagnaBind carboxyl derivatized beads were introduced into a microchannel, and then human immunoglobulin G (IgG) was bound to the bead surface in the microchannel of the chip. Immunoreaction was conducted in the microchannel for the bead-bounded antigen IgG with the antibody FITC-labelled IgG. On-chip detection was performed using a laser-induced fluorescence (LIF) system. The integration shortened the overall analysis time from 7 h to less than 40 min.
Chen
Tian
T
Department of Chemistry, Tsinghua University, Beijing 100084, People's Republic of China.
Lei
Jian-Du
JD
Tong
Ai-Jun
AJ
D000941:Antigens / Q000737:chemistry / Q000276:immunology; D002138:Calibration; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D007164:Immunosorbents / Q000276:immunology*; D056656:Lab-On-A-Chip Devices; D008832:Microchemistry; D046208:Microchip Analytical Procedures / Q000379:methods*; D008863:Microspheres
D000941:Antigens; D007074:Immunoglobulin G; D007164:Immunosorbents