title: [Experience with the PCR method in chlamydia detection.].
abstract: OBJECTIVE
  The study was intended to summarize and evaluate the results in patients with a suspected infection by the genus Chlamydia, investigated with an in-house method of nested PCR (polymerase chain reaction). The study worked with data from patients living in eastern Bohemia, who were examined in the years 2001-2003 at the Dept. of Molecular Biology, a research laboratory shared by the Institute of Clinical Microbiology and the Institute of Clinical Biochemistry and Diagnostics.
  
  
  METHODS
  291 explorations were done in 2001, in 2002 already 562 and in 2003 their figure reached 760. The total number of samples received for investigation during that period was 1 613. 1 587 were actually investigated, 26 were unsuitable and could not be used. More than 70 % of all investigations were done with three types of material: urine (41.8 % of all the investigated samples), BAL (15.3 % of all the investigated samples) and whole blood (14.9 % of all the investigated samples). The investigations were carried out with the in-house nested PCR method, which uses primers from the MOMP(ompA) area of the genus Chlamydia spp.
  
  
  RESULTS
  Total positivity was 5.67 %, in 1.26 % of the samples the resulted was considered uncertain and 93.07 % of the investigated samples were negative. In men PCR positivity was 6.11 %, in women 5.35 %. The major proportion of positive samples was from the age groups 70-79 years (11.67 %), 10-19 years (6.51 %) and 40-49 years (6.45 %). Overall positivity in smears from the urogenital system was 6.48 %, from urine 3.92 %, from BAL 10.70 % and from whole blood 5.51 %.
  
  
  BACKGROUND
  nested PCR, Chlamydia spp., detection.
authors.affiliation: Center of Biological Protection Techonin, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Fajfr
authors.forename: Miroslav
authors.initials: M
authors.lastname: Förstl
authors.forename: Vlasta
authors.initials: V
authors.lastname: Stepánová
authors.forename: Lenka
authors.initials: L
authors.lastname: Plísková
authors.forename: Jana
authors.initials: J
authors.lastname: Fajfrová
authors.forename: Zbyneek
authors.initials: Z
authors.lastname: Valenta
authors.forename: Miroslav
authors.initials: M
authors.lastname: Splino
mesh_terms: D002690:Chlamydia Infections; D002692:Chlamydia trachomatis; D006801:Humans; D016133:Polymerase Chain Reaction