[Effect of BSA on random amplified polymorphic DNA (RAPD) in plants].
Using Metasequoia glyptostroboides and Heptacodium miconioides DNA as templates,the effect of bovine serum albumin (BSA) on RAPD in plants was studied. The results showed that suitable concentrations of BSA used in Metasequoia glyptostroboides and Heptacodium miconioides RAPD were different, which were 0.6 microg/microl and 1 microg/microl, respectively. The inhibition of acetylated BSA on the amplification of plant RAPD could be relieved by BSA. BSA could reduce the dosage of Taq DNA polymerase.
Bian
Cai-Miao
CM
Biology and Chemistry Department, Taizhou Teachers College, Linhai, Zhejiang 317000, China. lijm@mail.tzptt.zj.cn
Li
Jun-Min
JM
Jin
Ze-Xin
ZX
Ge
Ming-Ju
MJ