Flow-injection-chemiluminescence method for the determination of penicillin G potassium.
The degradation product of penicillin G potassium can react with potassium permanganate in acidic medium and produce chemiluminescence, which is greatly enhanced by formaldehyde. The optimum conditions for this chemiluminescent reaction were studied in detail using a flow-injection system. The experiments indicated that under optimum conditions, the chemiluminescence intensity was linearly related to the concentration of penicillin G potassium within the range 1.0 x 10(-7)-1.0 x 10(-5) g/mL, with a detection limit (3sigma) of 7 x 10(-8) g/mL. The relative standard deviation was 1.0% for 4.0 x 10(-7) g/mL penicillin G potassium solution (n = 11). This method has the advantages of simple operation, fast response and high sensitivity. The method was successfully applied to the analysis of penicillin G potassium in raw medicines.
Cao
Wei
W
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
Yang
Jing-He
JH
Sun
Chang-Xia
CX
Zhang
Zhi-Juang
ZJ
Gao
Qing-Fei
QF
D017022:Flow Injection Analysis / Q000379:methods*; D005557:Formaldehyde / Q000737:chemistry; D006358:Hot Temperature; D006851:Hydrochloric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D015394:Molecular Structure; D010400:Penicillin G / Q000737:chemistry* / Q000378:metabolism; D011188:Potassium / Q000032:analysis* / Q000737:chemistry; D011196:Potassium Permanganate / Q000737:chemistry; D012680:Sensitivity and Specificity; D012972:Sodium Hydroxide / Q000737:chemistry
D011196:Potassium Permanganate; D005557:Formaldehyde; D012972:Sodium Hydroxide; D010400:Penicillin G; D006851:Hydrochloric Acid; D011188:Potassium