Gene expression profiling in single cells within tissue.
We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.
Capodieci
Paola
P
Aureon Laboratories, 28 Wells Avenue, Yonkers, New York 10701, USA. paola.capodieci@aureon.com
Donovan
Michael
M
Buchinsky
Heidi
H
Jeffers
Yusuf
Y
Cordon-Cardo
Carlos
C
Gerald
William
W
Edelson
Jon
J
Shenoy
Shailesh M
SM
Singer
Robert H
RH
D014408:Biomarkers, Tumor / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D016612:Paraffin Embedding / Q000379:methods*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins