A carotenoid cleavage dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation.
A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy-beta-ionone, a C(13)-norisoprenoidic compound, and a C(14)-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching véraison was observed in both cultivars. In parallel, the C(13)-norisoprenoid level increased from véraison to maturity in both cultivars.
Mathieu
Sandrine
S
UMR IR2B, ENSAM-INRA-Université Montpellier II, F-34060 Montpellier cedex 1, France.
Terrier
Nancy
N
Procureur
Jérôme
J
Bigey
Frédéric
F
Günata
Ziya
Z
D002247:Carbon Isotopes; D018076:DNA, Complementary / Q000235:genetics; D049308:Dioxygenases / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli; D020224:Expressed Sequence Tags; D005638:Fruit / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D045792:Norisoprenoids / Q000378:metabolism*; D010802:Phylogeny; D027843:Vitis / Q000201:enzymology* / Q000235:genetics*
D002247:Carbon Isotopes; D018076:DNA, Complementary; D045792:Norisoprenoids; D049308:Dioxygenases; C431939:carotenoid cleavage dioxygenase 1