[Determination of nitric oxide in serum and plasma of human blood. Method of high pressure liquid chromatography].
Two methods of measurement of nitrite and nitrate determination in human serum and plasma (a colorimetric method based on Griess reaction and a HPLC method) have been compared HPLC method revealed extremely low level of nitrites blood plasma, which was roughly the same (about 3 microM or less) in healthy donors, patients with diabetes and hypertension. The level of nitrates in the group of healthy donors (29.9 +/- 2.8 microM) is lower than in patients with diabetes (58.6 +/- 6.9 microM), and in patients with hypertension (35.4 +/- 2.5 microM). Comparison of data obtained by colorimetric and HPLC methods revealed close correlation (r = 0.77, p < 0.001). Methodical requirements for preparation of samples of serum and plasma of blood for analysis and correct intepretation of results are discussed in view of biochemical properties of nitric oxide molecule.
Kobylianskiĭ
A G
AG
Kuznetsova
T V
TV
Soboleva
G N
GN
Bondarenko
O N
ON
Pogorelova
O A
OA
Titov
V N
VN
Masenko
V P
VP
D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000097:blood; D006801:Humans; D008072:Hyperlipoproteinemia Type I / Q000097:blood; D006973:Hypertension / Q000097:blood; D009569:Nitric Oxide / Q000097:blood*; D010949:Plasma / Q000378:metabolism*; D044967:Serum / Q000378:metabolism*
D009569:Nitric Oxide