Anti-allergic effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on allergen-induced vascular permeability and hyperalgesia.
OBJECTIVE
We investigated the anti-allergic and analgesic properties of an oil and a derived fraction of tetranortriterpenoids (TNTP) obtained from the seeds of Carapa guianensis Aublet.


METHODS
Pleurisy, paw and ear edema were induced in Swiss and C57/Bl10 mice mice, whereas thermal hyperalgesia was assessed in Wistar rats (n = 6-10 per group). Values of p < 0.05 were regarded as significant.


RESULTS
C. guianensis oil (100 to 400 mg/kg, p.o.) and TNTP (12.5 to 100 mg/kg, p.o.) inhibited pleural exudation, paw and ear edema induced by ovalbumin (OVA) in sensitized mice. TNTP (12.5 to 100 mg/kg, p.o.) also inhibited paw edema induced by histamine, PAF and bradykinin. TNTP (100 mg/kg, p.o.) inhibited prostaglandin E(2) generation in the pleural cavity in response to antigenic challenge. Moreover, C. guianensis oil (100 to 400 mg/kg) and TNTP (12.5 to 100 mg/kg) decreased OVA- and histamine-induced hyperalgesia.


CONCLUSIONS
Taken together, these findings demonstrate the anti-edematogenic and analgesic effects of C. guianensis oil, and points out TNTP as the responsible bioactive compounds.
Penido
C
C
Departamento de Farmacologia Aplicada, Far-Manguinhos, Fundação Oswaldo Cruz, Rua Sizenando Nabuco 100, 21041-250 Rio de Janeiro, RJ, Brazil.
Costa
K A
KA
Pennaforte
R J
RJ
Costa
M F S
MF
Pereira
J F G
JF
Siani
A C
AC
Henriques
M G M O
MG
D000485:Allergens; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology; D001920:Bradykinin / Q000378:metabolism; D002199:Capillary Permeability; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004487:Edema; D006632:Histamine / Q000378:metabolism; D006930:Hyperalgesia; D007249:Inflammation; D036701:Limonins / Q000737:chemistry / Q000494:pharmacology*; D029582:Meliaceae / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008956:Models, Chemical; D010047:Ovalbumin / Q000737:chemistry / Q000494:pharmacology; D010539:Permeability; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000485:Allergens; D018926:Anti-Allergic Agents; D036701:Limonins; D010936:Plant Extracts; D006632:Histamine; D010047:Ovalbumin; D015232:Dinoprostone; D001920:Bradykinin