Involvement of the SCF complex in the control of Cdh1 degradation in S-phase.
The anaphase promoting complex/cyclosome (APC/C) is a multisubunit ubiquitin ligase that acts as a key regulator in the progression through mitosis (when mostly in complex with Cdc20) and as a stabilizer of the G1 phase (when in complex with Cdh1). Cdh1 is an activator of APC/C, and it has previously been reported that it is capable of mediating its own degradation during Go and G1. Herein, we show that the SCF complex (Skp1/Cul1/F-box protein/Roc1) intervenes in the surveillance of Cdh1 cellular abundance in S-phase.
Benmaamar
Ramla
R
Department of Pathology, New York University School of Medicine, New York, New York, USA. ramla.benmaamar@mssn.edu
Pagano
Michele
M
D020816:Amino Acid Motifs; D064173:Anaphase-Promoting Complex-Cyclosome; D064200:Cdh1 Proteins; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D004318:Doxycycline / Q000494:pharmacology; D016193:G1 Phase; D017353:Gene Deletion; D005799:Genes, Dominant; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008938:Mitosis; D011487:Protein Conformation; D034622:RNA Interference; D016192:Resting Phase, Cell Cycle; D016196:S Phase; D013997:Time Factors; D014162:Transfection; D043743:Ubiquitin-Protein Ligase Complexes / Q000502:physiology*; D014452:Ubiquitins / Q000737:chemistry
D064200:Cdh1 Proteins; D018797:Cell Cycle Proteins; C577584:FZR1 protein, human; D014452:Ubiquitins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D004318:Doxycycline