Effects of hold time after extracellular ice formation on intracellular freezing of mouse oocytes.
MII mouse oocytes in 1 and 1.5M ethylene glycol(EG)/phosphate buffered saline have been subjected to rapid freezing at 50 degrees C/min to -70 degrees C. When this rapid freezing is preceded by a variable hold time of 0-3 min after the initial extracellular ice formation (EIF), the duration of the hold time has a substantial effect on the temperature at which the oocytes subsequently undergo intracellular ice formation (IIF). For example, in 1M EG, the IIF temperatures are -23.7 and -39.2 degrees C with 0 and 2 min hold times; in 1.5M EG, the corresponding IIF temperatures are -29.1 and -40.8 degrees C.
Mazur
Peter
P
Fundamental and Applied Cryobiology Group, Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37932-2575, USA. pmazur@utk.edu
Pinn
Irina L
IL
Seki
Shinsuke
S
Kleinhans
Frederick W
FW
Edashige
Keisuke
K
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D015925:Cryopreservation / Q000379:methods*; D019855:Ethylene Glycol / Q000494:pharmacology; D005260:Female; D005615:Freezing; D007053:Ice; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
D007053:Ice; D019855:Ethylene Glycol