The phosphoprotein of attenuated measles AIK-C vaccine strain contributes to its temperature-sensitive phenotype.
Measles AIK-C vaccine strain exhibits temperature-sensitivity (ts). To identify the structural proteins, which contribute to the ts property of AIK-C virus, reverse genetics was used. MV-minigenome RNA was replicated at 32.5, 37, and 39 degrees C when the plasmids expressing N, P, and L proteins of the Edmonston strain (the parental strain of AIK-C) were used, whereas the minigenome RNA replicated only at 32.5 degrees C but did not at 37 degrees C and higher temperature when N, P, and L protein expression plasmids of the AIK-C strain were used. A series of minigenome experiments revealed that the amino acid substitution of leucine at position 439 of the P protein by proline (P439-Pro) contributes to the ts phenotype of AIK-C. Four recombinant viruses having various P genes were rescued from the modified AIK-C genome cDNA and ts-characteristics were compared in Vero cells by plaque formation assay. The results showed that the P439-Pro of AIK-C virus played a key role in the ts phenotype, but the other substitutions in the P gene might have an accessory function in the expression of the phenotype.
Komase
Katsuhiro
K
Division of Research and Development, Research Center for Biologicals, The Kitasato Institute, 6-111 Arai, Kitamoto-shi, Saitama 364-0026, Japan.
Nakayama
Tetsuo
T
Iijima
Masumi
M
Miki
Kenji
K
Kawanishi
Ryuta
R
Uejima
Hajime
H
D019943:Amino Acid Substitution; D000818:Animals; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D006367:HeLa Cells; D006801:Humans; D008459:Measles virus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D010750:Phosphoproteins / Q000737:chemistry / Q000276:immunology*; D010957:Plasmids; D012367:RNA, Viral / Q000096:biosynthesis; D011995:Recombination, Genetic; D013696:Temperature; D014709:Vero Cells; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000737:chemistry / Q000276:immunology*; D014779:Virus Replication
D010750:Phosphoproteins; D012367:RNA, Viral; D014764:Viral Proteins