[Effect of HBsAg pulsed dendritic cells on the functions of cytokine-induced killer cells].
OBJECTIVE
To explore the effects of HBsAg-pulsed dendritic cells (DCs) on the proliferation and killing functions of cytokine-induced killer (CIK) cells.


METHODS
The peripheral blood mononuclear cells (PBMCs) were isolated from the patients with chronic hepatitis B by the routine method, and induced into specific DCs with HBsAg pulsed. The (3)H-TdR incorporation method was used to determine the stimulation effect of HBsAg-pulsed DCs on the proliferation of CIK cells. LDH release assay was used to measure the specific killing activity of CIK cells on HepG2215 cells.


RESULTS
The HBsAg-pulsed DCs could induce the memory proliferation of CIK cells and strengthen the killing activity of CIK cells (P<0.05).


CONCLUSIONS
HBsAg-pulsed DCs can enhance the proliferation and killing functions of CIK cells.
Liu
Shu-ren
SR
Liver Disease Center of the PLA, Chinese PLA 458th Hospital, Guangzhou 510600, China. liushuren2002@cn.yahoo.com
Zhang
Yan
Y
Xie
Qing
Q
Li
Zhuo-liang
ZL
Yu
Zhou-yao
ZY
Kong
Xiang-ping
XP
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D055612:Cytokine-Induced Killer Cells / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology* / Q000502:physiology; D006801:Humans
D006514:Hepatitis B Surface Antigens