title: Aldosterone stimulates reactive oxygen species production through activation of NADPH oxidase in rat mesangial cells.
abstract: It has recently been shown that glomerular mesangial injury is associated with increases in renal cortical reactive oxygen species (ROS) levels in rats treated chronically with aldosterone and salt. This study was conducted to determine the mechanisms responsible for aldosterone-induced ROS production in cultured rat mesangial cells (RMC). Oxidative fluorescent dihydroethidium was used to evaluate intracellular production of superoxide anion (O(2)(-)) in intact cells. The lucigenin-derived chemiluminescence assay was used to determine NADPH oxidase activity. The staining of dihydroethidium was increased in a dose-dependent manner by aldosterone (1 to 100 nmol/L) with a peak at 3 h in RMC. Aldosterone (100 nmol/L for 3 h) also significantly increased NADPH oxidase activity from 232 +/- 18 to 346 +/- 30 cpm/5 x 10(4) cells. Immunoblotting data showed that aldosterone (100 nmol/L for 3 h) increased p47phox and p67phox protein levels in the membrane fraction by approximately 2.1- and 2.3-fold, respectively. On the other hand, mRNA expression of NADPH oxidase membrane components, p22phox, Nox-1, and Nox-4, were not altered by aldosterone (for 3 to 12 h) in RMC. Pre-incubation with the selective mineralocorticoid receptor (MR) antagonist, eplerenone (10 micromol/L), significantly attenuated aldosterone-induced O(2)(-) production, NADPH oxidase activation and membranous translocation of p47phox and p67phox. These results suggest that aldosterone-induced ROS generation is associated with NAPDH oxidase activation through MR-mediated membranous translocation of p47phox and p67phox in RMC. These cellular actions of aldosterone may play a role in the pathogenesis of glomerular mesangial injury.
authors.affiliation: Department of Pharmacology, Kagawa Medical University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
authors.forename: Kayoko
authors.initials: K
authors.lastname: Miyata
authors.forename: Matlubur
authors.initials: M
authors.lastname: Rahman
authors.forename: Takatomi
authors.initials: T
authors.lastname: Shokoji
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nagai
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Zhang
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Sun
authors.forename: Shoji
authors.initials: S
authors.lastname: Kimura
authors.forename: Tokihito
authors.initials: T
authors.lastname: Yukimura
authors.forename: Hideyasu
authors.initials: H
authors.lastname: Kiyomoto
authors.forename: Masakazu
authors.initials: M
authors.lastname: Kohno
authors.forename: Youichi
authors.initials: Y
authors.lastname: Abe
authors.forename: Akira
authors.initials: A
authors.lastname: Nishiyama
mesh_terms: D000450:Aldosterone / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D050527:Mesangial Cells / Q000378:metabolism*; D019255:NADPH Oxidases / Q000502:physiology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D000450:Aldosterone; D019255:NADPH Oxidases