[Antitumor immune response to cervix carcinoma in mice induced by HPV16 E6 DNA vaccines].
OBJECTIVE
To investigate the effect of HPV16E6 DNA vaccines on inducing the antitumor immune response to cervix carcinoma in vivo.


METHODS
We prepared pCB/HPV16E6 DNA vaccines to prevent and treat 615 modle mice transplanted E6 + U14 in vivo respectively. Survival and tumor sizes of all mice were recorded and their lymphnodes and lung's metastases were detected by microscopic observation. The cytotoxicity of the spleen T cells of those mice immuned with HPV E6 DNA vaccines against the E6 U14 and U14 was detected by MTT assay.


RESULTS
HPV16 E6 DNA vaccines prevented the transplanted tumor growth effectively in inbred strain mice and cured some tumor-bearing mice (P < 0. 001). Pathological results showed that the number of lymphodes with metastases foci decreased in the preventing group (P < 0.05). HPV16 E6 DNA vaccines induced special and higher cytotoxicity T lymphocytes against the E6 + U14 (P < 0.001).


CONCLUSIONS
HPV16 E6 DNA vaccines can induce antitumor immune protection of the mice against E6 + U14 cells, suggesting that the vaccines may be effective in preventing and treating cervix cancer after an operation.
Tao
Guang-Shi
GS
Department of Obstetrics and Gynecology, Second Xiangya Hospital, China.
Hu
Jin-Yue
JY
Liu
Feng-Ying
FY
D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D004279:DNA, Viral / Q000276:immunology; D005260:Female; D051379:Mice; D009368:Neoplasm Transplantation; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D012097:Repressor Proteins / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D002583:Uterine Cervical Neoplasms / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology*
D019496:Cancer Vaccines; D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins; D019444:Vaccines, DNA