Highly parallel microbial diagnostics using oligonucleotide microarrays.
Oligonucleotide microarrays are highly parallel hybridization platforms, allowing rapid and simultaneous identification of many different microorganisms and viruses in a single assay. In the past few years, researchers have been confronted with a dramatic increase in the number of studies reporting development and/or improvement of oligonucleotide microarrays for microbial diagnostics, but use of the technology in routine diagnostics is still constrained by a variety of factors. Careful development of microarray essentials (such as oligonucleotide probes, protocols for target preparation and hybridization, etc.) combined with extensive performance testing are thus mandatory requirements for the maturation of diagnostic microarrays from fancy technological gimmicks to robust and routinely applicable tools.
Loy
Alexander
A
Department of Microbial Ecology, University of Vienna, Austria. loy@microbia-ecology.net
Bodrossy
Levente
L
D001419:Bacteria / Q000302:isolation & purification*; D004783:Environmental Microbiology; D005819:Genetic Markers; D016678:Genome; D006801:Humans; D007239:Infections / Q000175:diagnosis / Q000382:microbiology / Q000821:virology; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D012680:Sensitivity and Specificity; D014780:Viruses / Q000302:isolation & purification*
D005819:Genetic Markers; D015345:Oligonucleotide Probes