title: The effect of dimyristoylphosphatidylethanol on the lateral and rotational mobilities of liposome lipid bilayers.
abstract: The aim of this study was to provide the basis to further examine the mode of action of ethanol. Fluorescent probes reported to have different membrane mobilities were used to evaluate the effect of dimyristoylphosphatidylethanol (DMPEt) on the lateral and rotational mobilities of liposome lipid bilayers. An experimental procedure, based on the selective quenching of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups, was used. DMPEt increased the bulk lateral and rotational mobilities, and had a greater fluidizing effect on the outer than the inner monolayer. These effects of DMPEt on liposomes may be responsible for some, but not all, of the general anesthetic actions of ethanol.
authors.affiliation: Research Institute for Oral Biotechnology, College of Dentistry, Pusan National University, Busan 602-739, Korea.
authors.forename: Hye-Ock
authors.initials: HO
authors.lastname: Jang
authors.forename: Min-Hoi
authors.initials: MH
authors.lastname: Huh
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Lee
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Lee
authors.forename: Jong-Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Jun-Bong
authors.initials: JB
authors.lastname: Seo
authors.forename: Kyo-Il
authors.initials: KI
authors.lastname: Koo
authors.forename: Seong-Deok
authors.initials: SD
authors.lastname: Jin
authors.forename: Je-Hyung
authors.initials: JH
authors.lastname: Jeong
authors.forename: Jang-Seop
authors.initials: JS
authors.lastname: Lim
authors.forename: Moon-Kyung
authors.initials: MK
authors.lastname: Bae
authors.forename: Il
authors.initials: I
authors.lastname: Yun
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D002492:Central Nervous System Depressants / Q000494:pharmacology; D004161:Diphenylhexatriene; D000431:Ethanol / Q000494:pharmacology; D020404:Glycerophospholipids / Q000494:pharmacology*; D006825:Hybridomas; D008051:Lipid Bilayers / Q000378:metabolism*; D008081:Liposomes; D008560:Membrane Fluidity / Q000187:drug effects*; D011721:Pyrenes; D013843:Thiazines
chemical_list: C104364:1,2-dimyristoyl-sn-glycero-3-phosphatidylethanol; D002492:Central Nervous System Depressants; D020404:Glycerophospholipids; D008051:Lipid Bilayers; D008081:Liposomes; D011721:Pyrenes; D013843:Thiazines; C053505:fluorescent dye D 282; D004161:Diphenylhexatriene; D000431:Ethanol; C035116:1,3-di-(1-pyrenyl)propane