title: [Effects of bone marrow fibroblasts conditioned media on the expansion of megakaryocytes in vitro].
abstract: OBJECTIVE
  To investigate the effect of bone marrow fibroblasts conditioned media on the expansion of megakaryocytes in vitro.
  
  
  METHODS
  Serum-free bone marrow fibroblasts conditioned medium (BMF-CM) was prepared and collected. The effect of BMF-CM or BMF-CM combined with IL-11 on the expansion of mature megakaryocytes and the effect of BMF-CM on the expansion of megakaryocytic progenitors were observed. RT-PCR was applied to determine the expression of NF-E2 mRNA in human megakaryoblastic leukemia cell line--Meg-0l.
  
  
  RESULTS
  BMF-CM promoted the expansion of mature megakaryocytes. The effect of BMF-CM combined with IL-11 on the expansion of mature megakaryocytes was further enhanced. BMF-CM promoted the expansion of megakaryocytic progenitors. NF-E2 mRNA expression in megakaryocytic cell lines Meg-0l was enhanced when cultures were exposed to 30% BMF-CM for 4 hours, compared with control cultures exposed to IMDM for 4 hours.
  
  
  CONCLUSIONS
  BMF-CM can promote the expansion of megakaryocytes in vitro.
authors.affiliation: Laboratory of Experimental Hematology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Zhou
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Huang
authors.forename: Qi-ru
authors.initials: QR
authors.lastname: Wang
authors.forename: Guang-xiu
authors.initials: GX
authors.lastname: Lu
authors.forename: Meng-qun
authors.initials: MQ
authors.lastname: Tan
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D002455:Cell Division; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology*; D014407:Tumor Cells, Cultured
chemical_list: D017077:Culture Media, Conditioned