Long form leptin receptor mRNA expression in the hypothalamus and pituitary during early pregnancy in the pig.
OBJECTIVE
The aim of this study was the detection and location of long form leptin receptor (OB-Rb) in different area of hypothalamus and pituitary in the pig during early pregnancy.


METHODS
Expression of OB-Rb was examined by RT-PCR in the different area of hypothalamus: medial basal hypothalamus (MBH), preoptic area (POA), stalk median eminence (SME), as well as pituitary: the anterior (AP) and posterior (NP) lobe collected from gilts at days 14-16 (n=4) and 30-32 (n=4) of pregnancy.


RESULTS
The results showed that OB-Rb mRNA was expressed in the hypothalamus (MBH, POA and SME), pituitary (AP, NP) and adipose tissue in the pig during early pregnancy (at days 14-16 and 30-32).


CONCLUSIONS
These findings support the idea that leptin might play a role in the regulation of the hypothalamic-pituitary axis activity, and consequently in the control of pregnancy during critical period of embryo implantation in the pig.
Siawrys
Gabriela
G
Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo, Poland. gabriela.siawrys@uwm.edu.pl
Przala
Jadwiga
J
Kaminski
Tadeusz
T
Smolinska
Nina
N
Gajewska
Alina
A
Kochman
Kazimierz
K
Skowronski
Mariusz
M
Staszkiewicz
Jaroslaw
J
D000818:Animals; D005260:Female; D007031:Hypothalamus / Q000502:physiology*; D007033:Hypothalamus, Middle / Q000502:physiology; D008473:Median Eminence / Q000502:physiology; D010903:Pituitary Gland, Anterior / Q000502:physiology*; D010904:Pituitary Gland, Posterior / Q000502:physiology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011301:Preoptic Area / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000235:genetics*; D054411:Receptors, Leptin; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin