[Cloning of the genes associated with the pathogenesis of pregnancy induced hypertension using subtractive hybridization].
OBJECTIVE
To clone the genes related to pregnancy induced hypertension (PIH) for the study of PIH pathogenesis.


METHODS
The differential expressed cDNA of PIH and normal pregnant placenta were cloned using modified polymerase chain reaction based subtractive hybridization. The differential cDNA were sequenced.


RESULTS
Among 86 clones from subtractive hybridization, 14 clones containing differential expressed gene fragments in PIH and normal placenta were identified, and these might be the genes associated with PIH. Compared with the expressed sequence tags (EST) in dbEST of National Center for Biotechnology Information, it showed that 11 of them were known gene fragments, and three were unknown. The three unknown gene fragments were registered in GenBank, the accession number is AF 232216, AF 232217, AF 233648 respectively.


CONCLUSIONS
Fourteen PIH related genes were cloned with subtractive hybridization. A new approach to study on the pathogenesis of PIH is presented.
Yao
Y
Y
Department of Obstetrics Hospital, Fourth Military Medical University, Xi'an 710038, China.
Shi
J
J
Yan
W
W
D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011247:Pregnancy; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary