Biocompatibility of EDTA, EGTA and citric acid.
This in vivo study evaluated, through the physicochemical assay method for quantification of enhanced vascular permeability, the irritating potential of EDTA, EGTA, citric acid and saline. Thirty-two male Wister rats were anesthetized and four experimental sites were demarcated on their backs. Injections of 2% Evans blue (20 mg/kg) were administered intravenously into the lateral caudal vein. The test solutions were immediately injected intradermally (0.01 mL) into the experimental sites. The animals were killed 30 min, 1, 3 and 6 h after injection of the solutions and each piece of skin was submerged in formamide and incubated at 45 masculineC for 72 h. After filtration, the optical density was measured in a spectrophotometer and the total amount of dye extracted from the samples was calculated by means of a standard calibration curve. Data were analyzed statistically by two-way ANOVA and Tukey's HSD test. Compared to control, EDTA had the greatest volume of dye followed by EGTA and citric acid, for all time periods. There were statistically significant differences between all solutions (p<0.01). Considering the periods assessed, a significant difference was observed between the 3- and 6-h groups (p<0.05), but not between the 30-min and 1-h groups. Among the organic acids evaluated in this study, citric acid yielded the lowest amount of extracted dye. This indicates that the citric acid was the least irritating solution.
Sousa
Simone Maria Galvão de
SM
Department of Materials Science and Metallurgy, Pontifical Catholic University, Rio de Janeiro, RJ, Brazil. ssousa@rdc.puc-rio.br
Bramante
Clovis Monteiro
CM
Taga
Eulázio Mikio
EM
D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D002199:Capillary Permeability / Q000187:drug effects*; D002614:Chelating Agents / Q000633:toxicity*; D019343:Citric Acid / Q000633:toxicity*; D004396:Coloring Agents; D004492:Edetic Acid / Q000633:toxicity*; D004533:Egtazic Acid / Q000633:toxicity*; D005070:Evans Blue; D007249:Inflammation / Q000139:chemically induced; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012388:Root Canal Irrigants / Q000633:toxicity*; D012965:Sodium Chloride / Q000633:toxicity
D001672:Biocompatible Materials; D002614:Chelating Agents; D004396:Coloring Agents; D012388:Root Canal Irrigants; D019343:Citric Acid; D012965:Sodium Chloride; D005070:Evans Blue; D004533:Egtazic Acid; D004492:Edetic Acid