Genetic characterization of a caprine pestivirus as the first member of a putative novel pestivirus subgroup.
Currently, the genus Pestivirus comprises four approved species, namely bovine viral diarrhoea viruses 1 and 2 (BVDV-1, BVDV-2), classical swine fever virus and border disease virus (BDV). Recently, three major genotypes have been identified within the species BDV and termed as subgroups BDV-1, BDV-2 and BDV-3. Here, an isolate from animals in a herd showing BD-like syndromes, which occurred in central Italy was analysed. A reverse transcriptase polymerase chain reaction was performed using primers that specifically amplify a fragment of the 5'-non-coding region (5'-NCR) from BDV. Both the 5'-NCR fragment and the entire Npro gene were sequenced and used for genetic typing. The 5'-NCR sequence revealed that the newly isolated Pestivirus could be allocated to the BDV species. Interestingly, the Npro sequence of this virus isolate significantly differed from all the ovine pestiviruses previously described, providing evidence for the presence of an additional subgroup within the species BDV.
De Mia
G M
GM
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Perugia, Italy. gm.demia@pg.izs.it
Greiser-Wilke
I
I
Feliziani
F
F
Giammarioli
M
M
De Giuseppe
A
A
D000818:Animals; D001882:Border Disease / Q000453:epidemiology* / Q000821:virology*; D018083:Border disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis*; D004196:Disease Outbreaks / Q000662:veterinary*; D006041:Goats; D007558:Italy / Q000453:epidemiology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep
D004279:DNA, Viral