Direct isolation of satellite cells for skeletal muscle regeneration.
Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.
Montarras
Didier
D
CNRS Unité de Recherche Associée 2578, Department of Developmental Biology, INSERM, Pasteur Institute, 75724 Paris Cedex 15, France. dmontarr@pasteur.fr
Morgan
Jennifer
J
Collins
Charlotte
C
Relaix
Frédéric
F
Zaffran
Stéphane
S
Cumano
Ana
A
Partridge
Terence
T
Buckingham
Margaret
M
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D002469:Cell Separation; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis; D003964:Diaphragm; D016189:Dystrophin / Q000096:biosynthesis; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000032:analysis; D018398:Homeodomain Proteins / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D018101:Mice, Inbred mdx; D008819:Mice, Nude; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology*; D000072041:PAX3 Transcription Factor; D051763:PAX7 Transcription Factor; D051761:Paired Box Transcription Factors; D012038:Regeneration; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000502:physiology / Q000637:transplantation; D033581:Stem Cell Transplantation; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis
D018952:Antigens, CD34; D004268:DNA-Binding Proteins; D016189:Dystrophin; D018398:Homeodomain Proteins; D000072041:PAX3 Transcription Factor; D051763:PAX7 Transcription Factor; D051761:Paired Box Transcription Factors; C497322:Pax7 protein, mouse; D014157:Transcription Factors; C068686:Pax3 protein, mouse; D049452:Green Fluorescent Proteins