Delayed stimulatory effect of low-intensity shockwaves on human periosteal cells.
We investigated the effect of shockwaves on cells explanted from normal human periosteum to study the potential mechanisms of their responses and to determine suitable treatment settings. The cells were subjected to one shockwave treatment with systematic combinations of energy intensities (range, 0.05-0.5 mJ/mm) and number of shocks (range, 500-2000) whereas control cells received no treatment. The immediate effect on cell viability and the long-lasting effect on proliferation, viable cell number at Day 18, and mineralization at Day 35 were assessed. We observed an immediate dose-dependent destructive effect of shockwaves. Energy intensity and number of shocks contributed equally to viability. Total energy dose (intensity x number of shocks) was a better reference for determining the shockwave effect. We also found a long-term stimulatory effect on proliferation, viable cell number, and calcium deposition of human periosteal cells. At the same total energy dose, low-intensity shockwaves with more shocks (0.12 mJ/mm at 1250 shocks) were more favorable for enhancing cellular activities than high-intensity waves with fewer shocks (0.5 mJ/mm at 300 shocks). These findings document some of the biochemical changes of periosteal cells during shockwave treatments.
Tam
Kam-Fai
KF
Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
Cheung
Wing-Hoi
WH
Lee
Kwong-Man
KM
Qin
Ling
L
Leung
Kwok-Sui
KS
D049109:Cell Proliferation / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D017102:Fracture Healing / Q000528:radiation effects*; D006801:Humans; D010012:Osteogenesis / Q000528:radiation effects; D010521:Periosteum / Q000166:cytology / Q000502:physiology / Q000528:radiation effects*; D014465:Ultrasonics / Q000009:adverse effects*; D014463:Ultrasonography / Q000009:adverse effects*