Series quartz crystal sensor for remote bacteria population monitoring in raw milk via the Internet.
A remote monitoring system based on a piezoelectric quartz crystal (SPQC) sensor was developed for the determination of the bacteria population in raw milk. The system employs the Windows XP server operating system, and its programs for data acquisition, display and transmission were developed using the LabVIEW 7.1 programming language. The circuit design consists of a circuit with a piezoelectric quartz crystal (SPQC) and a pair of electrodes. This system can provide dynamic data monitoring on a web-page via the Internet. Immersion of the electrodes in a cell culture with bacteria inoculums resulted in a change of frequency caused by the impedance change due to microbial metabolism and the adherence of bacteria on the surface of the electrodes. The calibration curve of detection times against density of bacteria showed a linear correlation coefficient (R(2) = 0.9165) over the range of 70-10(6) CFU ml(-1). The sensor could acquire sufficient data rapidly (within 4 h) and thus enabled real-time monitoring of bacteria growth via the Internet. This system has potential application in the detection of bacteria concentration of milk at dairy farms.
Chang
Ku-Shang
KS
Department of Food Science, Yuanpei University of Science and Technology, Taiwan, ROC. kushang@mail.yust.edu.tw
Jang
Hung-Der
HD
Lee
Ching-Fu
CF
Lee
Yuan-Guey
YG
Yuan
Chiun-Jye
CJ
Lee
Sheng-Hsien
SH
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002417:Cattle; D015169:Colony Count, Microbial / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D020407:Internet; D008892:Milk / Q000382:microbiology*; D011791:Quartz; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014584:User-Computer Interface
D011791:Quartz