A lipid raft-associated 67kDa laminin receptor mediates suppressive effect of epigallocatechin-3-O-gallate on FcepsilonRI expression.
(-)-Epigallocatechin-3-O-gallate (EGCG), a major green tea polyphenol, has previously exhibited a suppressive effect on the expression of the high-affinity IgE receptor (FcepsilonRI). This effect has been shown to be elicited by interaction with the plasma membrane microdomain lipid rafts. Recently, we have identified the 67 kDa laminin receptor (67LR) as a cell surface EGCG receptor that mediates an anti-cancer action. Here we show that the 67LR is highly associated with lipid rafts on human basophilic KU812 cells. Experiments using 67LR-enhanced and -reduced cells revealed that the EGCG's ability to downregulate FcepsilonRI expression correlated with the amount of 67LR. Thus, these results suggest that the lipid raft-associated 67LR plays an important role in mediating the FcepsilonRI-suppressive action of EGCG.
Fujimura
Yoshinori
Y
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Yamada
Koji
K
Tachibana
Hirofumi
H
D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015536:Down-Regulation; D006801:Humans; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism*; D008970:Molecular Weight; D017452:Receptors, IgG / Q000378:metabolism*; D017474:Receptors, Laminin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
C489017:FCGR1A protein, human; D017452:Receptors, IgG; D017474:Receptors, Laminin; D002392:Catechin; C045651:epigallocatechin gallate