[Study on the expression of human ERMAP gene in erythropoietic and macrophage differentiation of K562 cells].
In order to investigate the potential of human ERMAP gene in erythroid cell differentiation, K562 cells were induced to erythroid lineage by Ara-C and to macrophage lineage by TPA, human ERMAP mRNA was detected by fluorescent quantitative PCR. The results showed that human ERMAP mRNA increased while K562 cells were induced to erythroid lineage after treatment with Ara-C at 2.5 x 10(-6) mmol/L/L and 1.0 x 10(-6) mmol/L/L. Human ERMAP mRNA not changed while K562 cells were induced to macrophage lineage after treatment with TPA at 2.0 x 10(-6) mmol/L/L and 1.0 x 10(-6) mmol/L/L. It is concluded that human ERMAP gene plays an important role in differentiation and proliferation of erythroid cells.
He
Ying-Yi
YY
Department of Pediatrics, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.
Zhang
Xiao-Hong
XH
Ye
Tie-Zhen
TZ
Wu
Zi-Liang
ZL
D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001789:Blood Group Antigens / Q000235:genetics*; D000071797:Butyrophilins; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D003561:Cytarabine / Q000494:pharmacology; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D008264:Macrophages / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011990:Receptors, Transferrin / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D063268:Sialic Acid Binding Ig-like Lectin 3; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D001789:Blood Group Antigens; D000071797:Butyrophilins; C571163:CD33 protein, human; C068126:CD71 antigen; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D063268:Sialic Acid Binding Ig-like Lectin 3; C472715:scianna antigens; D003561:Cytarabine; D013755:Tetradecanoylphorbol Acetate