title: Spectrofluorimetric assay of ciprofloxacin hydrochloride in tablets.
abstract: OBJECTIVE
  To describe the spectrofluorimetric method for the determination of ciprofloxacin hydrochloride in tablets under optimum conditions.
  
  
  METHODS
  Examination by spectrofluorimetry.
  
  
  RESULTS
  The wavelengths of excitation and emission were 290 and 450 nm, respectively. The fluorescence intensity was linearly related to the drug concentration over the range 2-8 microg/mL, with a limit of detection of 1.4 microg/mL and a limit of quantitation of 2.0 microg/mL. The method was highly accurate and precise, having a relative SD of < 1.0 %. The validity of the method was tested by recovery studies of the standard addition to pharmaceuticals, and the results were satisfactory. The results obtained by the application of this method and the official one were in good agreement, and statistical comparison by Student's t test and the variance ratio F test showed no significant difference between the 2 methods.
  
  
  CONCLUSIONS
  The proposed method is simple and sufficiently precise for quality control purposes.
authors.affiliation: Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey. zdurmus@science.ankara.edu.tr
authors.forename: Zehra
authors.initials: Z
authors.lastname: Durmus
authors.forename: Esin
authors.initials: E
authors.lastname: Canel
authors.forename: Esma
authors.initials: E
authors.lastname: Kiliq
mesh_terms: D002939:Ciprofloxacin / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
chemical_list: D013607:Tablets; D002939:Ciprofloxacin