Interfacial ternary complex DNA/Ca/lipids at anionic vesicle surfaces.
The electroporative transfer of gene DNA and other bioactive substances into tissue cells by electric pulses gains increasing importance in the new disciplines of electrochemotherapy and electrogenetherapy. The efficiency of the electrotransfer depends crucially on the adsorption of the gene DNA and oligonucleotides to the plasma cell membranes. Here it is shown that the adsorption of larger oligonucleotides such as fragments (ca. 300 bp) of sonicated calf-thymus DNA, to anionic lipids of unilamellar vesicles (diameter Phi=300+/-90 nm) is greatly enhanced by divalent cations such as Ca(2+)-ions. Applying centrifugation, bound and free DNA are monitored optically at the wavelength lambda=260 nm. Using arsenazo III as a Ca(2+)-indicator and atomic absorption spectroscopy (AAS), Ca(2+)-titrations of DNA and vesicles yield the individual equilibrium constants of Ca(2+)- and DNA-binding not only for the binary complexes: Ca/lipids, Ca/DNA and DNA/lipids, respectively, but also for the various processes to form the ternary complex DNA/Ca/lipids. The data provide the basis for goal-directed optimization protocols for the adsorption and thus efficient electrotransfer of oligonucleotides and polynucleotides into cells.
Frantescu
Alina
A
Physical and Biophysical Chemistry, Faculty of Chemistry, University of Bielefeld, P. O. Box 100131, D-33615 Bielefeld, Germany.
Tönsing
Katja
K
Neumann
Eberhard
E
D000838:Anions; D002118:Calcium / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004058:Diffusion; D018274:Electroporation / Q000379:methods*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
D000838:Anions; D008051:Lipid Bilayers; D008081:Liposomes; D004247:DNA; C427203:calf thymus DNA; D002118:Calcium