[Effects of carbon sources on the expression of cry1D -lacZ in Bacillus thuringiensis].
In order to investigate the influence of the carbon source on the expression of cry1D -lacZ in Bacillus thuringiensis with different genetic backgrounds, cry1D -lacZ was introduced into different strains and cultured in liquid medium containing different carbon sources. Analysis of activity of beta-galactosidase showed that cry1D -lacZ expressed differently in the same host because of different carbon sources and differently in different hosts utilizing same carbon sources,suggesting that metabolism pathway of carbon source in HD-133-5 was special. Sodium succinate as carbon source could lead to earlier sporulation and earlier expression of cry1D -lacZ. When sodium acetate acted as carbon source, cry1D -lacZ could keep high, stable expression in different hosts. These results are helpful for future fermentation of Bacillus thuringiensis.
Zhang
Li-Li
LL
Huazhong Agricultural University, Key Laboratory of Agricultural Microbiology, Ministry of Agriculture, Wuhan 430070, China. zhanglyly@yahoo.com
Arthur
Aronson I
AI
Yu
Zi-Niu
ZN