[Multiple resistance of neisseria gonorrhoeae and mutations in the inverted sequence within the mtrR promoter region].
OBJECTIVE
To evaluate the correlation between multiple resistance of Neisseria gonorrhoeae and mutations in the inverted sequence within the mtrR promoter region.


METHODS
The susceptibilities of 56 Neisseria gonorrhoeae to 5 antibiotic agents were tested by disc diffusion method. 13 bp inverted sequences positioned within the mtrR promoter region were amplified by PCR and examined by single strand conformation polymorphism technology. According to the results of SSCP, 12 strains were selected and were directly sequenced, and their nucleotide sequences were compared with those of susceptible strain ATCC19 424.


RESULTS
In the 56 strains of Neisseria gonorrhea, 5 strains were susceptible to all antibiotics and 22 strains were resistant to one antibiotic agent. In the 19 strains that were resistant to 2 antibiotic agents, 2 had mutations in a 13 bp inverted sequence positioned within the mtrR promoter region. Another 7, 2 and 1 strain which was resistant to 3,4 and 5 antibiotic agents respectively all had mutations in a 13 bp inverted sequence positioned within the mtrR promoter region. All of the 12 strains which contained the same mutation exhibited a single base pair deletion in a 13 bp inverted sequence positioned within the mtrR promoter region.


CONCLUSIONS
Deletions in the 13 bp inverted sequence positioned within the mtrR promoter region mediate multiple resistances in Neisseria gonorrhoeae. A single base pair deletion in a 13 bp inverted sequence positioned within the mtrR promoter region is associated with multiple resistance.
Wang
Dong-mei
DM
Department of Microbiology, Xiangya School of Medicine, Changsha 410078.
Xia
Zhong-di
ZD
Qi
Su-wen
SW
Zou
Ming-xiang
MX
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006069:Gonorrhea / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D017384:Sequence Deletion
D001426:Bacterial Proteins; D012097:Repressor Proteins; C087450:mtrR protein, Neisseria gonorrhoeae