title: Establishment and characterization of human glioblastoma cell line (HUBT-n).
abstract: We succeeded in primary culture of 3 in 4 cases of glioblastomas. The long-term passage cultures were not done from the primary cultures of original tumor, but glioblastoma cell line (HUBT-n) was established from a xenograft of nude mouse. This line grew well without interruption for 4 years and was subcultivated over 120 times. The cells were spindle like or round in shape and neoplastic and pleomorphic features contained glial fibrillar acid protein (GFAP) and S-100 protein and grew multilayering without contact inhibition. A bough-shaped long projection was noted from a small cell. One of the characteristics of the HUBT-n cells was existence of well developed intermediate filaments in their cytoplasm. The cells proliferated rapidly, and the population doubling time was about 32 hours. The chromosome number showed a narrow distribution of diploid range. Abnormal constitution was observed in all cells by G-band karyotyping. The culture cells were easily transplanted into the subcutis of nude mouse and produced the tumor resembling the original tumor.
authors.affiliation: Ishiwata Obstetrics and gynecology Hosp.
authors.forename: Isamu
authors.initials: I
authors.lastname: Ishiwata
authors.forename: Chieko
authors.initials: C
authors.lastname: Ishiwata
authors.forename: Emiko
authors.initials: E
authors.lastname: Ishiwata
authors.forename: Megumi
authors.initials: M
authors.lastname: Iguchi
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Sato
authors.forename: Makoto
authors.initials: M
authors.lastname: Sonobe
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kiguchi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tachibana
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
mesh_terms: D000368:Aged; D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D009368:Neoplasm Transplantation