Kinetic modeling of acetophenone reduction catalyzed by alcohol dehydrogenase from Thermoanaerobacter sp.
NADPH-dependent alcohol dehydrogenase (ADH) from Thermoanaerobacter sp. was kinetically characterized using reduction of acetophenone as a model. To achieve 98% conversion of acetophenone, cofactor regeneration by oxidation of 2-propanol with the same enzyme was used. The enzyme was stable in the batch reactor. It was enantioselective towards (S)-1-phenylethanol (ee>99.5%). Due to its high deactivation in continuously operated stirred tank reactor (kd=0.0141 min-1) there was no way to keep high conversion of acetophenone at 98%. The deactivation occurred in the repetitive batch as well. A mathematical model for the acetophenone reduction with cofactor regeneration describing the behaviour in a batch, repetitive-batch and continuously stirred tank reactor was developed.
Findrik
Zvjezdana
Z
Faculty of Chemical Engineering and Technology, University of Zagreb, Savska c. 16, HR-10000, Zagreb, Croatia.
Vasic'-Racki
Durda
D
Lütz
Stephan
S
Daussmann
Thomas
T
Wandrey
Christian
C
D019840:2-Propanol / Q000737:chemistry; D000098:Acetophenones / Q000737:chemistry*; D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D002384:Catalysis; D002849:Chromatography, Gas; D007700:Kinetics; D008956:Models, Chemical; D008962:Models, Theoretical; D009249:NADP / Q000737:chemistry / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000737:chemistry; D046968:Thermoanaerobacter / Q000201:enzymology / Q000378:metabolism*; D013997:Time Factors
D000098:Acetophenones; D009249:NADP; D000426:Alcohol Dehydrogenase; D010626:Phenylethyl Alcohol; D019840:2-Propanol; C038699:acetophenone; D010100:Oxygen