Ovarian cancer marker of 11.7 kDa detected by proteomics is a serum amyloid A1.
In this study, to reduce the number of major plasma components, we examined thermostable plasma fractions to search for a biomarker of ovarian cancer. An apparent cancer biomarker of 11.7 kDa was detected in these fractions using ProteinChip SELDI-TOF mass spectrometry system. This peak invariably appeared with another close peak of about 11.5 kDa, suggesting that it is a derivative of a larger mass molecule. Of 27 cancer plasma specimens, 15 (55.6%) demonstrated this peak pair, whereas only 2 of 34 controls specimens (5.8%) were shown to express it with low intensity. Using a method involving cysteine modification by 4-vinylpyridine (4-VP), 2-DE and HPLC, these peaks were identified by mass spectrometry as serum amyloid A1 (11.68 kDa) and its N-terminal arginine-truncated form (11.52 kDa).
Moshkovskii
Sergei A
SA
Institute of Biomedical Chemistry, Moscow, Russia. sergei.moshkovskii@ibmc.msk.ru
Serebryakova
Marina V
MV
Kuteykin-Teplyakov
Konstantin B
KB
Tikhonova
Olga V
OV
Goufman
Eugene I
EI
Zgoda
Victor G
VG
Taranets
Irina N
IN
Makarov
Oleg V
OV
Archakov
Alexander I
AI
D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000097:blood* / Q000175:diagnosis; D040901:Proteomics; D000685:Serum Amyloid A Protein / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D000685:Serum Amyloid A Protein