title: Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues.
abstract: Lymphocytes travel throughout the body to carry out immune surveillance and participate in inflammatory reactions. Their path takes them from blood through tissues into lymph and back to blood. Molecules that control lymphocyte recruitment into extralymphoid tissues are well characterized, but exit is assumed to be random. Here, we showed that lymphocyte emigration from the skin was regulated and was sensitive to pertussis toxin. CD4(+) lymphocytes emigrated more efficiently than CD8(+) or B lymphocytes. T lymphocytes in the afferent lymph expressed functional chemokine receptor CCR7, and CCR7 was required for T lymphocyte exit from the skin. The regulated expression of CCR7 by tissue T lymphocytes may control their exit, acting with recruitment mechanisms to regulate lymphocyte transit and accumulation during immune surveillance and inflammation.
authors.affiliation: Department of Pathology, Stanford University School of Medicine, Stanford University, Stanford, California 94305, USA. debes@stanford.edu
authors.forename: Gudrun F
authors.initials: GF
authors.lastname: Debes
authors.forename: Carrie N
authors.initials: CN
authors.lastname: Arnold
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Young
authors.forename: Stefan
authors.initials: S
authors.lastname: Krautwald
authors.forename: Martin
authors.initials: M
authors.lastname: Lipp
authors.forename: John B
authors.initials: JB
authors.lastname: Hay
authors.forename: Eugene C
authors.initials: EC
authors.lastname: Butcher
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D008196:Lymph / Q000276:immunology* / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D042601:Lymphatic Vessels / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D054400:Receptors, CCR7; D019707:Receptors, Chemokine / Q000172:deficiency / Q000276:immunology* / Q000378:metabolism; D012756:Sheep; D012867:Skin / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: C515371:Ccr7 protein, mouse; D054400:Receptors, CCR7; D019707:Receptors, Chemokine