Effect of standardized extract of Ocimum sanctum Linn. on gastric mucosal offensive and defensive factors.
The standardized methanolic extract of leaves of O. sanctum (OSE; eugenol content 5%) given in doses of 50-200 mg/kg, orally, twice daily for five days showed dose-dependent ulcer protective effect against cold restraint stress induced gastric ulcers. Optimal effective dose (100 mg/kg) of OSE showed significant ulcer protection against ethanol and pyloric ligation-induced gastric ulcers, but was ineffective against aspirin-induced ulcers. OSE significantly healed ulcers induced by 50% acetic acid after 5 and 10 days treatment OSE (100 mg/kg) significantly inhibited the offensive acid-pepsin secretion and lipid peroxidation and increased the gastric defensive factors like mucin secretion, cellular mucus, and life span of mucosal cells and had antioxidant effect, but did not induce mucosal cell proliferation. The results indicate that the ulcer protective and healing effects of OSE may be due to its effects both on offensive and defensive mucosal factors.
Goel
R K
RK
Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India. rkgoel_bhu@yahoo.co.in
Sairam
K
K
Dorababu
M
M
Prabha
T
T
Rao
Ch V
ChV
D000284:Administration, Oral; D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D031339:Ocimum / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism; D014456:Ulcer / Q000188:drug therapy* / Q000209:etiology
D000897:Anti-Ulcer Agents; D005609:Free Radicals; D010936:Plant Extracts; D004247:DNA; D002374:Catalase; D013482:Superoxide Dismutase