title: Secretory immunoglobulin A in saliva of women with oral and genital HPV infection.
abstract: Secretory IgA contributes towards the protection of mucosal surfaces against invading microorganisms.
  
  
  OBJECTIVE
  Quantify secretory IgA titers in the saliva of women with HPV in the oropharynx and/or in the genital area.
  
  
  METHODS
  Seventy women with clinical genital HPV lesions and 70 women without HPV infection were tested for oral HPV DNA and the levels of total IgA in their saliva. One millilitre of saliva was collected, centrifuged and stored at -80 degrees C for the measurement of secretory IgA by nephelometry technique. A pool of oral pharyngeal cells was collected for HPV identification by polymerase chain reaction.
  
  
  RESULTS
  Oral HPV PCR was positive in 29 (21%) women (26 women with genital HPV and only 3 women without genital HPV). Titers of secretory IgA were extremely lower in-patients with HPV DNA in the oropharynx when compared to HPV negative women (p<0.0001). Genital HPV and smoking were also associated to low levels of total sIgA in saliva (p<0.01). After multivariable analyses only the presence of HPV in the oral cavity and/or in genital area, but not smoking, was related to low levels of total secretory IgA.
  
  
  CONCLUSIONS
  Women with low levels of total secretory IgA could be more susceptible to having their oral mucosa colonized by HPV.
authors.affiliation: The State University of Campinas, School of Medical Sciences, São Paulo, Brazil.
authors.forename: Ana Katherine S
authors.initials: AK
authors.lastname: Gonçalves
authors.forename: Paulo
authors.initials: P
authors.lastname: Giraldo
authors.forename: Silvia
authors.initials: S
authors.lastname: Barros-Mazon
authors.forename: Marisa Lins
authors.initials: ML
authors.lastname: Gondo
authors.forename: Rose Luce
authors.initials: RL
authors.lastname: Amaral
authors.forename: Cláudia
authors.initials: C
authors.lastname: Jacyntho
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005836:Genitalia, Female / Q000821:virology*; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000302:isolation & purification*; D009055:Mouth / Q000821:virology*; D009061:Mouth Mucosa; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012463:Saliva / Q000276:immunology*
chemical_list: D004279:DNA, Viral; D007071:Immunoglobulin A, Secretory