[Role of dendritic cells in the pathogenesis of allergic rhinitis in mouse].
OBJECTIVE
To investigate the role of dendritic cells in the pathogenesis of acute attack of allergic rhinitis in mouse.


METHODS
The adherent precursors of DC were isolated from peripheral blood of allergic rhinitis mouse in acute attack stage and healthy controls. The adherent cells were induced with CpG ODN-1826 to DC in vitro. The expression of the surface molecules CD80, CD86, HLA-DR etc. on the DC was examined by fluorescent activated cell sorter (FACS), so the ability of DCs secrete IL-10, IL-12.


RESULTS
The results showed that the allergic rhinitis mouse in acute attack stage had remarkably decreased the ability to secret IL-10 compared with normal control subjects (P <0.01), while the ability to secret IL-12 remarkably decreased compared with normal control subjects (P < 0.01); meanwhile, the HLA-DR and co-stimulating factor CD86 expressed by DCs remarkably increased in the allergic rhinitis mouse in acute attack stage compared with the normal control subjects (P < 0.01).


CONCLUSIONS
DC possibly plays a vital role in the immunological mechanism of allergic rhinitis by means of inducing the differentiation of Th1/Th2, that is DC may be the key factor in initiating the airway allergic reaction and the possible mechanism may involve interleukins (especially IL-10 and IL-12, etc.) secreted by DCs.
Xie
Jun
J
Department of Otorhinolaryngology, Tongji Hospital, Tongji Medical College, HuazhongUniversity of Science and Technology, Wuhan, 430030, China.
Liao
Hui
H
Liu
Jie
J
Liu
Lisi
L
D000818:Animals; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism; D018664:Interleukin-12 / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012220:Rhinitis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology
D016753:Interleukin-10; D018664:Interleukin-12