Fluorinated diamond-like carbon as antithrombogenic coating for blood-contacting devices.
Diamond-like carbon (DLC) is being considered for widespread clinical use as a surface coating for cardiovascular devices. We synthesized fluorinated DLC (F-DLC) coatings in order to create a more hydrophobic surface with improved antithrombogenicity and flexibility when compared with conventional DLC coatings by combining the inertness of DLC films with the advantage of fluorination. The purpose of this study was to evaluate the in vitro hemocompatibility and in vivo biocompatibility of the F-DLC coating for medical devices. The in vitro whole blood model confirmed that platelet loss was lower in the F-DLC group than in the noncoated group (SUS316L), which suggests the adhesion of a smaller number of platelets to F-DLC-coated materials. Furthermore, the biomarkers of mechanically induced platelet activation (beta-thromboglobulin) and activated coagulation (thrombin-antithrombin-three complex) were markedly reduced in the F-DLC-coated group. In vivo rat implant model studies revealed no excessive local and systemic inflammatory responses in the F-DLC group. The thickness of the fibrous tissue capsule surrounding the F-DLC-coated disk was almost equal to that of the noncoated SUS316L disk, which has the favorable biocompatibility for metallic implant materials. F-DLC coating thus appears to be a promising candidate for use as a coating material in blood-contacting devices.
Hasebe
Terumitsu
T
Department of Radiology, Tachikawa Hospital, 4-2-22 Nishiki-cho, Tachikawa, Tokyo, Japan 190-8531. teru_hasebe@hotmail.com
Shimada
Atsushi
A
Suzuki
Tetsuya
T
Matsuoka
Yoshiaki
Y
Saito
Toshiya
T
Yohena
Satoshi
S
Kamijo
Aki
A
Shiraga
Nobuyuki
N
Higuchi
Mutsumi
M
Kimura
Kanako
K
Yoshimura
Hirokuni
H
Kuribayashi
Sachio
S
D000818:Animals; D001769:Blood; D002244:Carbon; D020099:Coated Materials, Biocompatible; D018130:Diamond; D005343:Fibrinolytic Agents; D005461:Fluorine; D005549:Foreign-Body Reaction / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000473:pathology; D008297:Male; D008422:Materials Testing; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D013927:Thrombosis / Q000097:blood / Q000517:prevention & control*
D020099:Coated Materials, Biocompatible; D005343:Fibrinolytic Agents; D005461:Fluorine; D002244:Carbon; D018130:Diamond