Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by use of Chelex 100 resin and polymerase chain reaction.
A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.
Thill
Charles
C
State of New York Department of Health, Centers for Molecular Medicine, Stony Brook University, Stony Brook, NY 11794-5120, USA.
Backenson
P Bryon
PB
Prusinski
Melissa A
MA
Kogut
Sarah J
SJ
Lee
Joon-Hak
JH
Coleman
James L
JL
D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology; D041001:Babesia microti / Q000235:genetics*; D002614:Chelating Agents; D016054:DNA, Protozoan / Q000032:analysis*; D018884:Ixodes / Q000469:parasitology*; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D012117:Resins, Synthetic
D002614:Chelating Agents; D016054:DNA, Protozoan; D012117:Resins, Synthetic; C024997:Chelex 100