[Identification of markers linked to resistance locus of Marssonina leaf spot in poplars by bulked segregant analysis(BSA)].
DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis(BSA). The bulks consisted of individual with a extreme phenotype taken from a population of 91 F1 clones,which is a progeny of Populus deltoides Bartr.cv."Lux"(I-69/55)(Resistance) and P.euramericana cv.I-45(Susceptible). Out of 114 RAPD primers, four markers showed polymorphisms between the resistance-bulk and the susceptible-bulk.By using selective genotype linkage analysis,OPAI17-1550 and OPAI13-900 were found linked to the resistance locus. The genetic distances between the two markers and the resistance locus were 29.9cM and 37.4cM,respectively.
Zhang
Bo
B
The Key Laboratory of Forest Tree Genetic Engineering, Nanjing Forestry University, Nanjing,210037, China. zhangbo1978@hotmail.com
Huang
Min-ren
MR
Zhuge
Qiang
Q
Han
Zheng-min
ZM
Yin
Tong-ming
TM
Pan
Hui-xin
HX
Zhu
Li-huang
LH
Wu
Rong-ling
RL
Wang
Ming-xiu
MX