Spatially controlled co-culture of neurons and glial cells.
The ability to create and maintain neuron and glial cell co-cultures is important for neuronal regeneration as well as for fundamental studies on neuron and glial cell interactions. We demonstrate here a method for spatially controlling the arrangement of neurons and glial cells. Line patterns of cell resistant, poly(oligoethyleneglycol methacrylate-co-methacrylic acid), was microcontact printed on various substrates to spatially control the attachment of neurons. Neuron-like cells, PC12 and SH-SY5Y cells, were confined within the unprinted line patterns and extended neurites along the line patterns. Subsequent attachment of glial cells was accomplished by converting the originally cell-resistant line patterns of poly(oligoethyleneglycol methacrylate-co-methacrylic acid) to cell adhesive by electrostatic adsorption of cationic poly-lysine, chitosan, or poly(ethyleneimine). This method for creating patterned co-cultures of neuron and glial cells provides a useful tool for investigating neuron-glial cell interactions and has potential applications in the repair or regeneration of nervous systems.
Yang
In Hong
IH
Department of Chemical and Material Engineering, University of Cincinnati, Cincinnati, Ohio 45221-0012, USA.
Co
Carlos C
CC
Ho
Chia-Chi
CC
D000818:Animals; D001672:Biocompatible Materials; D045744:Cell Line, Tumor; D048271:Chitosan; D018920:Coculture Techniques; D006801:Humans; D019344:Lactic Acid; D009457:Neuroglia; D009474:Neurons; D016716:PC12 Cells; D011091:Polyesters; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D011108:Polymers; D011109:Polymethacrylic Acids; D051381:Rats
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D011109:Polymethacrylic Acids; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); D011094:Polyethyleneimine; D048271:Chitosan