title: [Effects of bone marrow endothelial cell conditioned medium on the expansion of mature megakaryocytes and CFU-Meg in vitro].
abstract: OBJECTIVE
  To investigate the effects of serum-free bone marrow endothelial cell conditioned medium (E-CM) and serum-free bone marrow fibroblast conditioned medium (F-CM) on the expansion of mature megakaryocytes and CFU-Meg .
  
  
  METHODS
  E-CM and F-CM were collected and added in liquid culture system respectively.
  
  
  RESULTS
  E-CM and F-CM significantly promoted the expansion of mature megakaryocytes and CFU-Meg in liquid culture system. The effect of E-CM on the expansion of CFU-Meg was better than the effect of F-CM, While the effect of E-CM on the expansion of mature megakaryocytes was inferior to the effect of F-CM.
  
  
  CONCLUSIONS
  E-CM can be used to expand CFU-Meg while F-CM can be used to expand the mature megakaryocytes in vitro.
authors.affiliation: Research Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Huang
authors.forename: Qi-Ru
authors.initials: QR
authors.lastname: Wang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000378:metabolism; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D008297:Male; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice
chemical_list: D003115:Colony-Stimulating Factors; D017077:Culture Media, Conditioned