Spectrofluorimetric assay of ciprofloxacin hydrochloride in tablets.
OBJECTIVE
To describe the spectrofluorimetric method for the determination of ciprofloxacin hydrochloride in tablets under optimum conditions.


METHODS
Examination by spectrofluorimetry.


RESULTS
The wavelengths of excitation and emission were 290 and 450 nm, respectively. The fluorescence intensity was linearly related to the drug concentration over the range 2-8 microg/mL, with a limit of detection of 1.4 microg/mL and a limit of quantitation of 2.0 microg/mL. The method was highly accurate and precise, having a relative SD of < 1.0 %. The validity of the method was tested by recovery studies of the standard addition to pharmaceuticals, and the results were satisfactory. The results obtained by the application of this method and the official one were in good agreement, and statistical comparison by Student's t test and the variance ratio F test showed no significant difference between the 2 methods.


CONCLUSIONS
The proposed method is simple and sufficiently precise for quality control purposes.
Durmus
Zehra
Z
Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey. zdurmus@science.ankara.edu.tr
Canel
Esin
E
Kiliq
Esma
E
D002939:Ciprofloxacin / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
D013607:Tablets; D002939:Ciprofloxacin