[Improvement of concentration method for detecting genotoxic activities of drinking water using umuC-test].
umuC-test is an effective method to determine the genotoxic activity. In this study, the highest genotoxic activity was found exactly at 37 degrees C by modifying original condition of umuC test. Effects of concentration methods on the genotoxic activity was studied, and it was found that the HLB solid phase extraction is most effective to extract the genotoxic chemicals from a drinking water when using acetone as eluant. The fate of genotoxic activity in drinking water treatment process was also studied, and chlorination promoted the induction of genotoxic activity.
Gu
Wei-min
WM
College of Environmental Science, Peking University, Beijing 100871, China.
Hu
Jian-ying
JY
Wang
Wei
W
Liu
Yan-hua
YH
He
Wen-jie
WJ
Han
Hong-da
HD
D004259:DNA-Directed DNA Polymerase; D004784:Environmental Monitoring / Q000379:methods; D029968:Escherichia coli Proteins; D009152:Mutagenicity Tests / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014881:Water Supply / Q000032:analysis*; D001616:beta-Galactosidase / Q000378:metabolism*
D029968:Escherichia coli Proteins; D014874:Water Pollutants, Chemical; C078202:UmuC protein, E coli; D004259:DNA-Directed DNA Polymerase; D001616:beta-Galactosidase