R(1rho) relaxation for two-site chemical exchange: general approximations and some exact solutions.
Analytic expressions for the nuclear magnetic spin relaxation rate constant for magnetization spin-locked in the rotating reference frame under an applied radiofrequency field, R(1rho), are obtained for two-site chemical exchange. The theoretical approach is motivated by Laguerre's method and obtains R(1rho) as the root of a (p1,q1) Padé approximant. The general formula for R(1rho) obtained by this approach is substantially simpler than existing expressions and is equally or slightly more accurate, in most cases. In addition, particular solutions for the R(1rho) rate constant are presented for two special cases: equal populations of the two exchanging sites, or placement of the radiofrequency carrier at the average resonance frequency of the two sites. The solutions are exact when the R1 and R2 relaxation rate constants are identical, and nearly exact under realistic experimental conditions.
Miloushev
Vesselin Z
VZ
Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, USA.
Palmer
Arthur G
AG
D008956:Models, Chemical; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry
D011506:Proteins