Phosphoproteomic analysis of the effect of cyclo-[His-Pro] dipeptide on PC12 cells.
The effects of dipeptide cyclo-[His-Pro] (CHP), known to participate in the appetite behavior and food intake control, have been investigated using PC12 cells in culture as model system. We found that only in the presence of experimental conditions that cause cellular stress the cyclic dipeptide affect cellular proliferation and protects from apoptosis. It greatly enhances the phosphorylation of hsp27, alpha-B-crystallin, Cdc2, and p-38 MAPK, whereas it decreases the phosphorylation of MEK1, Cav 2, GSK3a, PKB/Akt, PKCdelta, PKCgamma, and Erk2. PKA and PKG are involved in ERK1/2 deactivation via a receptor that appears to be dually coupled to Gs and Gq protein subfamilies.
Minelli
Alba
A
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Perugia, via del Giochetto, 06123 Perugia, Italy. albaminelli@virgilio.it
Bellezza
Ilaria
I
Grottelli
Silvia
S
Pinnen
Francesco
F
Brunetti
Luigi
L
Vacca
Michele
M
D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D004151:Dipeptides / Q000737:chemistry* / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology; D016716:PC12 Cells; D010456:Peptides, Cyclic / Q000502:physiology*; D010750:Phosphoproteins / Q000502:physiology*; D010879:Piperazines; D040901:Proteomics; D051381:Rats
D004151:Dipeptides; D018696:Neuroprotective Agents; D010456:Peptides, Cyclic; D010750:Phosphoproteins; D010879:Piperazines; C015924:histidyl-proline diketopiperazine