Yeast transformation by the LiAc/SS Carrier DNA/PEG method.
The technique for the transformation of Saccharomyces cerevisiae using the LiAc/SS Carrier DNA/PEG method is described. We describe a rapid method, for use when large numbers of transformants are not necessary. A high-efficiency method for the generation of large numbers of transformants is also given. A method for the transformation of plasmid libraries, which includes yeast two-hybrid applications, also is listed to aid the reader in generating transformants to effectively cover the library complexity. Finally, a protocol for transformation using a 96-well format is included for transformation applications that require it.
Gietz
R Daniel
RD
Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Canada.
Woods
Robin A
RA
D000085:Acetates; D004274:DNA, Recombinant / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D015723:Gene Library; D009172:Mycology / Q000379:methods; D010957:Plasmids / Q000235:genetics; D011092:Polyethylene Glycols; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques
D000085:Acetates; D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D011092:Polyethylene Glycols; C488804:lithium acetate