Effects of pixantrone on immune-cell function in the course of acute rat experimental allergic encephalomyelitis.
Pixantrone is an immunesuppressor similar to mitoxantrone but with lower cardiotoxicity. We evaluated the effect of pixantrone on B cells and lymphomononuclear cells in the course of acute EAE. Pixantrone reduced the number of B cells and suppressed myelin basic protein (MBP) specific IgG production. In vitro, pixantrone induced apoptosis of rat B lymphocytes in a way similar to mitoxantrone. In addition, pixantrone inhibited antigen specific and mitogen induced lymphomononuclear cell proliferation, as well as IFN-gamma production, during EAE. These findings suggest a similar mechanism of action for pixantrone and mitoxantrone on the effector function of lymphomonocyte B and T cells.
Mazzanti
Benedetta
B
Department of Neurological and Psychiatric Sciences, University of Florence, Florence, Italy.
Biagioli
Tiziana
T
Aldinucci
Alessandra
A
Cavaletti
Guido
G
Cavalletti
Ennio
E
Oggioni
Norberto
N
Frigo
Maura
M
Rota
Stefania
S
Tagliabue
Elena
E
Ballerini
Clara
C
Massacesi
Luca
L
Riccio
Paolo
P
Lolli
Francesco
F
D000704:Analysis of Variance; D000818:Animals; D000906:Antibodies / Q000097:blood; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D048749:Cytokinesis; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D007546:Isoquinolines / Q000627:therapeutic use*; D004676:Myelin Basic Protein / Q000008:administration & dosage / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000187:drug effects; D013997:Time Factors
D000906:Antibodies; D015703:Antigens, CD; D016207:Cytokines; D007166:Immunosuppressive Agents; D007546:Isoquinolines; D004676:Myelin Basic Protein; C086548:pixantrone