title: Transcriptional signaling from membrane raft-associated glucocorticoid receptor.
abstract: The contribution of plasma membrane-associated glucocorticoid receptor (GR) to transcriptional signaling is unclear. We observed GR in low-density detergent-resistant membrane (DRM) rafts derived from human hepatoma Hep3B cells in complexes with caveolin-1, HSP90, and STAT3. In transient transfection assays, GR-stimulated transcriptional signaling was reversibly inhibited by membrane-raft disrupters filipin III and progesterone. These data provide clear evidence for a functional contribution of DRM-associated GR to transcriptional signaling.
authors.affiliation: Department of Pathology, New York Medical College, Valhalla, NY 10595, USA.
authors.forename: Sudhir
authors.initials: S
authors.lastname: Jain
authors.forename: Yanna
authors.initials: Y
authors.lastname: Li
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.forename: Pravin B
authors.initials: PB
authors.lastname: Sehgal
mesh_terms: D001483:Base Sequence; D051242:Caveolin 1; D022461:Caveolins / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D005372:Filipin / Q000494:pharmacology; D006801:Humans; D017830:Octoxynol / Q000737:chemistry; D009841:Oligonucleotides; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; D009841:Oligonucleotides; D011965:Receptors, Glucocorticoid; D005372:Filipin; D017830:Octoxynol