title: Elevated circulating platelet-derived microparticles in patients with active inflammatory bowel disease.
abstract: OBJECTIVE
  Platelet-derived microparticles (PDMPs) are active molecules involved in the hemostatic and inflammatory responses. To evaluate the changes in the platelet function in patients with inflammatory bowel disease (IBD), we measured circulating PDMP levels.
  
  
  METHODS
  Twenty-five healthy controls, 44 patients with ulcerative colitis (UC), and 43 patients with Crohn's Disease (CD) were studied. The PDMP and soluble P-selectin (sP-selectin) levels were measured by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  In the healthy controls, the PDMP levels were 17.2 +/- 6.2 U/mL. Significant differences were not observed between the healthy controls and inactive UC patients (20.8 +/- 9.5 U/mL, n = 25) or between the healthy controls and inactive CD patients (17.6 +/- 7.8 U/mL, n = 24). In contrast, the PDMP levels were significantly higher in both active UC (49.2 +/- 33.6 U/mL, n = 19) and active CD (48.6 +/- 42.8 U/mL, n = 19) patients than in the healthy controls. A significant correlation was found between the PDMP levels and the clinical activity indexes (CAI) of UC patients (r = 0.65, p < 0.01, n = 44), and between the PDMP levels and Crohn's disease activity indexes (CDAI) (r = 0.72, p < 0.01, n = 43). Elevated PDMP levels in active patients were significantly reduced after remission. A significant correlation was observed between the PDMP levels and the sP-selectin levels (r = 0.60, p < 0.01, n = 122).
  
  
  CONCLUSIONS
  Elevated circulating PDMPs in active IBD patients suggest a role for platelets in the pathogenesis of IBD.
authors.affiliation: Department of Internal Medicine, Shiga University of Medical Science, Seta Tukinowa, Otsu, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Andoh
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Tsujikawa
authors.forename: Kazunori
authors.initials: K
authors.lastname: Hata
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Araki
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Kitoh
authors.forename: Masaya
authors.initials: M
authors.lastname: Sasaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Fujiyama
mesh_terms: D000208:Acute Disease; D000328:Adult; D001792:Blood Platelets / Q000502:physiology / Q000648:ultrastructure*; D003093:Colitis, Ulcerative / Q000097:blood*; D003424:Crohn Disease / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D019007:P-Selectin / Q000097:blood
chemical_list: D019007:P-Selectin