[New non-nucleoside substrates for terminal deoxynucleotidyl transferase: the synthesis and the dependence of substrate properties on structure].
N-(9-Fluorenylmethoxycarbonyl)-omega-aminoalkyl, N-(9-fluorenylmethoxycarbonyl)-8-amino-3,6-dioxaoctyl, and N-(9-fluorenylmethoxycarbonyl)-6-aminohexanoyl]-2-aminoethyl triphosphates were synthesized. All of them were shown to be the substrates of the calf thymus terminal deoxynucleotidyl transferase. Their substrate properties depend on the length and structure of linker between the 9-fluorenylmethoxycarbonyl and triphosphate moieties.
Khandazhinskaia
A L
AL
Kukhanova
M K
MK
Ias'ko
M V
MV
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D015394:Molecular Structure; D010755:Organophosphates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013950:Thymus Gland / Q000201:enzymology
D010755:Organophosphates; D004253:DNA Nucleotidylexotransferase