Induction of apoptotic cell death by 2'-hydroxycinnamaldehyde is involved with ERK-dependent inactivation of NF-kappaB in TNF-alpha-treated SW620 colon cancer cells.
2'-Hydroxycinnamaldehyde (HCA) inhibits cell growth of several human cancer cells with unknown mechanisms. We investigated the inhibitory effect of HCA on TNF-alpha-induced cell growth and possible signal pathway in SW620 colon cancer cells. HCA inhibited TNF-alpha-induced SW620 colon cell growth in time- and dose-dependent manner through induction of apoptotic cell death. Parallel with inhibitory effect on cell growth, HCA dose dependency inhibited TNF-alpha-induced activation of NF-kappaB accompanied with inhibition of the translocation of p50. HCA also induced expression of caspase-3 and Bax, but decreased Bcl-2. HCA furthermore activated ERK pathway, and ERK inhibitor reversed inhibitory effect of HCA on cell growth and transcriptional activation of NF-kappaB. These results demonstrate that HCA inhibits cell growth through induction of apoptotic cell death by ERK pathway-dependent NF-kappaB inactivation.
Lee
Seung Ho
SH
College of Pharmacy, Chungbuk National University, Heungduk-gu, Cheongju, South Korea.
Lee
Chung Woo
CW
Lee
Jae Woong
JW
Choi
Myoung Suk
MS
Son
Dong Ju
DJ
Chung
Youn Bok
YB
Lee
Chong Kil
CK
Oh
Ki Wan
KW
Moon
Dong Chul
DC
Kwon
Byoung Mog
BM
Hong
Jin Tae
JT
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002934:Cinnamates / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology*; D024202:Electrophoretic Mobility Shift Assay; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D002934:Cinnamates; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C117045:p-hydroxycinnamaldehyde; D048049:Extracellular Signal-Regulated MAP Kinases