title: Localization of two nitric oxide synthase isoforms, eNOS and iNOS, in the skin of Triturus italicus (Amphibia, Urodela) during development.
abstract: We analyzed the skin of Triturus italicus (Amphibia, Urodela) histologically during the larval, pre-metamorphic and adult phases in parallel with the immunohistochemical evaluation of the expression of two nitric oxide synthase (NOS) isoforms, i.e. the inducible NOS (iNOS) and the endothelial NOS (eNOS). Our results indicate that, during the larval and adult phases, substantial changes in the intensity and localization of both iNOS and eNOS are present. In contrast, the pre-metamorphic newts show a labelling pattern similar to that found in the skin of adult individuals. These data suggest an involvement of the NOS system in the larval epidermis during functional maturation phase starting at the climax and preceding structural rearrangements during metamorphosis, emphasizing the putative functional importance of differential isoform expression related to a distinct phase of the biological cycle.
authors.affiliation: Department of Ecology, University of Calabria, Via P. Bucci, I-87036, Rende, Cosenza, Italy. brunelli@unical.it
authors.forename: Elvira
authors.initials: E
authors.lastname: Brunelli
authors.forename: Ida
authors.initials: I
authors.lastname: Perrotta
authors.forename: Erminia
authors.initials: E
authors.lastname: Talarico
authors.forename: Sandro
authors.initials: S
authors.lastname: Tripepi
mesh_terms: D000818:Animals; D004847:Epithelial Cells / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D008675:Metamorphosis, Biological; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis* / Q000737:chemistry; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis* / Q000737:chemistry; D020033:Protein Isoforms; D012867:Skin / Q000196:embryology / Q000378:metabolism; D013997:Time Factors; D014319:Triturus / Q000196:embryology*
chemical_list: D020033:Protein Isoforms; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III