Photochemiluminescent detection of antiradical activity. VII. Comparison with a modified method of thermo-initiated free radical generation with chemiluminescent detection.
The method of photosensitized chemiluminescence (PCL) allows the quantification of water- and lipid-soluble antioxidants and activity of superoxide dismutase (SOD) in the same measuring system. However, it needs a special device, which we have described in a previous paper in this series. Another method suitable for the assay of water- and lipid-soluble antioxidants is the thermo-initiated decay of azo-compounds combined with the measurement of O2 consumption (Niki, 1985; Wayner et al., 1985). Its long duration and the complicated measuring procedure is not acceptable for routine medical applications. We show that a modification using CL detection of free radicals with luminol, has results comparable with PCL for the determination of non-enzymic water- and lipid-soluble antioxidants, SOD activity and oxidative modification of proteins. In contrast to PCL, it is possible to use any luminometer with a heatable measuring cell and to investigate coloured samples. While the new method has an overall higher sensitivity and is scalable to microtitre plates, PCL measurements can be made at different pH. The advantages and analytical information content of certain components of the integral antioxidative capacity of blood plasma are discussed in comparison with other methods.
Popov
I
I
Research Institute for Antioxidant Therapy GmbH, Berlin, Germany. ip@antioxidant-research.com
Lewin
G
G
D001205:Ascorbic Acid / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D002138:Calibration; D003920:Diabetes Mellitus / Q000097:blood; D005609:Free Radicals / Q000037:antagonists & inhibitors* / Q000097:blood / Q000378:metabolism*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D010777:Photochemistry; D017319:Photosensitizing Agents; D012995:Solubility; D013696:Temperature; D014527:Uric Acid / Q000097:blood; D014867:Water
D001798:Blood Proteins; D005609:Free Radicals; D017319:Photosensitizing Agents; D014867:Water; D014527:Uric Acid; D008165:Luminol; D001205:Ascorbic Acid