Effects of copper and zinc on the production of homocysteine-induced extracellular matrix metalloproteinase-2 in cultured rat vascular smooth muscle cells.
OBJECTIVE
To study the effects of copper and zinc on the production of homocysteine-induced extracellular matrix metalloproteinase-2 in cultured rats vascular smooth muscle cells.


METHODS
We examined the effects of different homocysteine levels (0-1000 micromol/l) on matrix metalloproteinase-2 (MMP-2) production, and the effects of different copper and zinc concentrations (0-300 micromol/l) on homocysteine-induced MMP-2 in cultured rat vascular smooth muscle cells (VSMCs) using gelatin zymography and western blotting. We further compared the changes of MMP-2 under various treatments for 24, 48 and 72 hours.


RESULTS
Homocysteine (50-1000 micromol/l) increased the production of MMP-2 significantly in a dose-dependent manner. Copper decreased the homocysteine-induced MMP-2 under these conditions in a dose-dependent manner, but zinc did not. Production of MMP-2 under various treatments for 72 h increased more than 24 h and 48 h.


CONCLUSIONS
Extracellular added copper decreased homocysteine-induced MMP-2 secretion.
Guo
Hangyuan
H
Department of Cardiology, Huayu Hospital, China Medical College, Shaoxin, Zhejiang, 312030, China. ghangyuan@hotmail.com
Lee
Jong-Dae
JD
Uzui
Hiroyasu
H
Toyoda
Kiyohiro
K
Geshi
Tooru
T
Yue
Hong
H
Ueda
Takanori
T
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology*; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D006710:Homocysteine / Q000494:pharmacology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015032:Zinc / Q000494:pharmacology*
D006710:Homocysteine; D003300:Copper; D020778:Matrix Metalloproteinase 2; D015032:Zinc