Analysis of cellular phenotypes that mediate genetic resistance to tuberculosis using a radiation bone marrow chimera approach.
Adoptive transfer of bone marrow cells from tuberculosis-resistant (I/St x A/Sn)F(1) donor mice into lethally irradiated susceptible I/St recipients changed their phenotype following infection with virulent Mycobacterium tuberculosis. Compared to I/St-->I/St control animals, F(1)-->I/St chimeras demonstrated (i) prolonged survival time, (ii) increased antimycobacterial function of lung macrophages, (iii) elevated gamma interferon production by lung cells, and (iv) decreased infiltration of the lungs with CD4(+) and CD8(+) T cells and Ly-6G(+) neutrophils.
Majorov
Konstantin B
KB
Laboratory for Immunogenetics, Central Institute for Tuberculosis, Yauza alley 2, Moscow 107564, Russia.
Eruslanov
Evgeny B
EB
Rubakova
Elvira I
EI
Kondratieva
Tatiana K
TK
Apt
Alexander S
AS
D019264:Adoptive Transfer; D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology*; D016026:Bone Marrow Transplantation / Q000276:immunology / Q000473:pathology; D005260:Female; D020022:Genetic Predisposition to Disease; D016130:Immunophenotyping; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008805:Mice, Inbred A; D010641:Phenotype; D011828:Radiation Chimera; D014397:Tuberculosis, Pulmonary / Q000235:genetics* / Q000276:immunology* / Q000401:mortality / Q000473:pathology