Analyses of benzophenones by capillary electrochromatography using methacrylate ester-based monolithic columns.
In this study, eight benzophenones, which are commonly used as UV filters in various cosmetics and plastics, were analyzed by capillary electrochromatography with a methacrylate ester-based monolithic column. The effects of the composition and pH of mobile phase, porogenic solvent ratio, and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) content on benzophenone separations were examined. For all benzophenones, separation performances were markedly improved in monolithic columns with larger 1-propanol ratio and higher AMPS content. Furthermore, a twofold increase in AMPS content almost reduced the separation time in half when a monolithic column had an adequately high surface area, i.e. monolithic column was produced in a higher ratio of 1-propanol. As well, the retention behaviors of these analytes in the monolithic column were strongly influenced by the level of acetonitrile in the mobile phase, and the pH of the mobile phase also had an apparent influence on separation resolution.
Huang
Hsi-Ya
HY
Department of Chemistry and Center for Nano Technology, Chung Yuan Christian University, Chung-Li, Taiwan. hyhuang@cycu.edu.tw
Chiu
Chen-Wen
CW
Huang
I Yun
IY
Lee
Szetsen
S
D001577:Benzophenones / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D004952:Esters; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D012997:Solvents
D001577:Benzophenones; D004952:Esters; D008689:Methacrylates; D012997:Solvents