title: [Effects of long-term replacement therapy of compound nylestriol tablet or low-dose 17 beta-estradiol on the expression of nerve growth factor in OVX rat hippocampal formation].
abstract: OBJECTIVE
  To determine the effects of long-term estrogen deficiency and replacement therapy of compound nylestriol tablet or low-dose 17 beta-estradiol on the expression of nerve growth factor (NGF) in rat hippocampal formation.
  
  
  METHODS
  Fifty 7 month-old female Sprague-Dawley rats were randomly divided into 5 groups: normal control, sham operated (SHAM), ovariectomized (OVX), OVX plus 17 beta-estradiol (OVX/ERT), and OVX plus compound nylestriol tablet (OVX/NL) groups. Immunohistochemistry of NGF was used to quantitatively determine the levels of expression of NGF using cell counting and imaging system in ovariectomized rat hippocampal formation.
  
  
  RESULTS
  The number and optical density of NGF-positive neurons of all hippocampal subregions and dentate gyrus in OVX rats were obviously lower than those of the normal control, SHAM, OVX/NL, and OVX/ERT rats.
  
  
  CONCLUSIONS
  Long-term estrogen deficiency can lead to a decrease of NGF expression in hippocampal formation, while the replacement of low-dose 17 beta-estradiol or compound nylestriol tablet can equally preserve the expression of NGF to a normal level, showing a neurotrophic effect of estrogen.
authors.affiliation: Department of Neurology, Second Xiangya Hospital, Central South University, Changsha 410011, China. Jiangbao2002@yahoo.com.cn
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Er-yuan
authors.initials: EY
authors.lastname: Liao
authors.forename: Li-ming
authors.initials: LM
authors.lastname: Tan
authors.forename: Ru-chun
authors.initials: RC
authors.lastname: Dai
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Xiao
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Liao
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000378:metabolism; D004958:Estradiol / Q000008:administration & dosage*; D015914:Estrogen Replacement Therapy; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D010052:Ovariectomy; D011800:Quinestrol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004958:Estradiol; C010722:nylestriol; D020932:Nerve Growth Factor; D011800:Quinestrol