Simultaneous determination of adenine and guanine in ruminant bacterial pellets by ion-pair HPLC.
An ion-pair reversed-phase high-performance liquid chromatography with gradient elution and UV detection was used to measure adenine (A) and guanine (G) in lyophilized bacterial pellets from ruminants using allopurinol as internal standard. The separation was performed on a Symmetry C18 column and the detection was monitored at 280 nm. Calibration curves were found to be linear in the concentration range from 5 to 50 mg/l with correlation coefficients (r2)>0.999. Mean recoveries of A and G standards added to bacterial samples were 102.2 and 98.2, respectively. The method proposed yielded sharp, well-resolved peaks within 25 min and was successfully applied for the determination of A and G in bacterial pellets.
García del Moral
Pilar
P
Analytical Chemistry, Department of Biochemistry and Molecular Biology, Campus de Vegazana s/n, University of León, 24071 León, Spain.
Arín
María Jesús
MJ
Resines
José Antonio
JA
Díez
María Teresa
MT
D000225:Adenine / Q000032:analysis*; D000818:Animals; D001419:Bacteria / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006147:Guanine / Q000032:analysis*; D012417:Rumen / Q000382:microbiology
D006147:Guanine; D000225:Adenine