Genome-wide screening for genetic changes in a matched pair of benign and prostate cancer cell lines using array CGH.
Copy number alterations in a matched pair of benign epithelial and prostate cancer cell lines derived from the same patient were assessed using array-based comparative genomic hybridisation (aCGH). The cancer cell line showed a gain of chromosome 7, deletion of chromosome 8, gains (including high level) and losses on chromosome 11, loss of 18p and gain of 20q. Deletions on chromosome 8 were confirmed with microsatellite markers. The aCGH results were compared to gene expression data obtained using DNA microarrays and suggested the involvement of caspases and ICEBERG on 11q and E2F1 on chromosome 20q.
Brookman-Amissah
N
N
Prostate Cancer Research Centre, Institute of Urology, University College London, London, UK.
Duchesnes
C
C
Williamson
M P
MP
Wang
Q
Q
Ahmed
A
A
Feneley
M R
MR
Mackay
A
A
Freeman
A
A
Fenwick
K
K
Iravani
M
M
Weber
B
B
Ashworth
A
A
Masters
J R
JR
D045744:Cell Line, Tumor; D002877:Chromosomes, Human / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005820:Genetic Testing / Q000379:methods*; D015894:Genome, Human; D006579:Heterozygote; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics; D008297:Male; D046228:Microarray Analysis; D018895:Microsatellite Repeats / Q000235:genetics; D009693:Nucleic Acid Hybridization; D011467:Prostate / Q000166:cytology* / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*