Differentiation of Candida albicans and Candida dubliniensis using a single-enzyme PCR-RFLP method.
Candida dubliniensis is a novel Candida spp. that is similar to Candida albicans with respect to several phenotypic characteristics. However, they differ from each other with respect to epidemiology, pathogenesis, and the rapid development of resistance to fluconazole. In the present study, we used a single-enzyme PCR-restriction fragment length polymorphism (RFLP) technique to differentiate C. dubliniensis from C. albicans. The amplified ITS region of C. dubliniensis was digested once using the enzyme B1nI, whereas that of C. albicans remained intact. All standard strains tested were identified successfully by this method. None of 140 clinical isolates identified morphologically as C. albicans were recognized as C. dubliniensis based on their PCR-RFLP pattern. Our PCR-RFLP method easily differentiated C. dubliniensis from C. albicans, and this result was also demonstrated with standard strains.
Mirhendi
Hossein
H
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. mirhendi@yahoo.com
Makimura
Koichi
K
Zomorodian
Kamiar
K
Maeda
Nobuko
N
Ohshima
Tomoko
T
Yamaguchi
Hideyo
H
D000328:Adult; D002175:Candida / Q000235:genetics / Q000302:isolation & purification*; D002176:Candida albicans / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis* / Q000382:microbiology; D004251:DNA Transposable Elements / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D006801:Humans; D008297:Male; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D004251:DNA Transposable Elements; C064053:CCTAGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific