title: Morphine promotes phosphorylation of the human delta-opioid receptor at serine 363.
abstract: After prolonged stimulation, the delta-opioid receptor becomes desensitized by regulatory mechanisms such as receptor phosphorylation, internalization and down-regulation. In this study, we demonstrate that morphine treatment causes phosphorylation of S363 in the C-terminus of the human delta-opioid receptor. Morphine-mediated phosphorylation reached 53+/-8% of maximum deltorphin II-mediated phosphorylation. Phosphorylation of S363 may contribute to delta-opioid receptor desensitization by morphine.
authors.affiliation: Department of Medical Pharmacology, College of Medicine, The University of Arizona, Tucson, AZ 85724, USA.
authors.forename: Edita
authors.initials: E
authors.lastname: Navratilova
authors.forename: Miriam C
authors.initials: MC
authors.lastname: Eaton
authors.forename: Dagmar
authors.initials: D
authors.lastname: Stropova
authors.forename: Eva V
authors.initials: EV
authors.lastname: Varga
authors.forename: Todd W
authors.initials: TW
authors.lastname: Vanderah
authors.forename: William R
authors.initials: WR
authors.lastname: Roeske
authors.forename: Henry I
authors.initials: HI
authors.lastname: Yamamura
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D009020:Morphine / Q000494:pharmacology*; D009154:Mutation; D009842:Oligopeptides / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D017465:Receptors, Opioid, delta / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D012694:Serine / Q000235:genetics / Q000378:metabolism
chemical_list: D009842:Oligopeptides; D017465:Receptors, Opioid, delta; C060209:deltorphin II, Ala(2)-; D012694:Serine; D009020:Morphine