title: [Effect of HBsAg pulsed dendritic cells on the functions of cytokine-induced killer cells].
abstract: OBJECTIVE
  To explore the effects of HBsAg-pulsed dendritic cells (DCs) on the proliferation and killing functions of cytokine-induced killer (CIK) cells.
  
  
  METHODS
  The peripheral blood mononuclear cells (PBMCs) were isolated from the patients with chronic hepatitis B by the routine method, and induced into specific DCs with HBsAg pulsed. The (3)H-TdR incorporation method was used to determine the stimulation effect of HBsAg-pulsed DCs on the proliferation of CIK cells. LDH release assay was used to measure the specific killing activity of CIK cells on HepG2215 cells.
  
  
  RESULTS
  The HBsAg-pulsed DCs could induce the memory proliferation of CIK cells and strengthen the killing activity of CIK cells (P<0.05).
  
  
  CONCLUSIONS
  HBsAg-pulsed DCs can enhance the proliferation and killing functions of CIK cells.
authors.affiliation: Liver Disease Center of the PLA, Chinese PLA 458th Hospital, Guangzhou 510600, China. liushuren2002@cn.yahoo.com
authors.forename: Shu-ren
authors.initials: SR
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Xie
authors.forename: Zhuo-liang
authors.initials: ZL
authors.lastname: Li
authors.forename: Zhou-yao
authors.initials: ZY
authors.lastname: Yu
authors.forename: Xiang-ping
authors.initials: XP
authors.lastname: Kong
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D055612:Cytokine-Induced Killer Cells / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology* / Q000502:physiology; D006801:Humans
chemical_list: D006514:Hepatitis B Surface Antigens