Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems.
Low molecular weight peptides obtained from ultrafiltration (UF) of giant squid (Dosidicus gigas) muscle protein were studied for their antioxidative effects in different in vitro oxidative systems. The most potent two peptides, Asn-Ala-Asp-Phe-Gly-Leu-Asn-Gly-Leu-Glu-Gly-Leu-Ala (1307 Da) and Asn-Gly-Leu-Glu-Gly-Leu-Lys (747 Da), exhibited their antioxidant potential to act as chain-breaking antioxidants by inhibiting radical-mediated peroxidation of linoleic acid, and their activities were closer to highly active synthetic antioxidant, butylated hydroxytoluene. Addition of these peptides could enhance the viability of cytotoxic embryonic lung fibroblasts significantly (P<.05) at a low concentration of 50 microg/ml, and it was presumed due to the suppression of radical-induced oxidation of membrane lipids. Electron spin trapping studies revealed that the peptides were potent scavengers of free radicals in the order of carbon-centered (IC(50) 396.04 and 304.67 microM), hydroxyl (IC(50) 497.32 and 428.54 microM) and superoxide radicals (IC(50) 669.34 and 573.83 microM). Even though the exact molecular mechanism for scavenging of free radicals was unclear, unusually high hydrophobic amino acid composition (more than 75%) of giant squid muscle peptides was presumed to be involved in the observed activities.
Rajapakse
Niranjan
N
Department of Chemistry, Pukyong National University, Busan 608-737, South Korea.
Mendis
Eresha
E
Byun
Hee-Guk
HG
Kim
Se-Kwon
SK
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D049832:Decapodiformes; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004798:Enzymes / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000166:cytology / Q000187:drug effects; D008563:Membrane Lipids / Q000378:metabolism; D009132:Muscles / Q000737:chemistry; D010084:Oxidation-Reduction; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D018675:Toxicity Tests; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D000975:Antioxidants; D004798:Enzymes; D016166:Free Radical Scavengers; D005609:Free Radicals; D008563:Membrane Lipids; D010455:Peptides; D020122:tert-Butylhydroperoxide