title: Antitumor response against myeloma cells by immunization with mouse syngenic dendritoma.
abstract: In order to generate an immune response against myeloma cells in an homogenous murine model, a stable hybrid cell line (DCSp) was established through the syngenic fusion between mouse dendritic cells (DC) and mouse Sp2/0 myeloma cells. DCSp cells behaved as potent T cell stimulators and were able to induce Sp2/0 specific cytotoxicity. When mice were immunized with irradiated hybrids before SP2/0 injection, they exhibited a significantly higher rate of survival as compared with controls. When tumors were detected, their emergence was not delayed, and time elapsed between tumor clinical perception and death remained unchanged. A humoral immune response was also always associated. We assume that this stable dendritoma cell line can be considered a valuable tool for myeloma studies in an homogenous mouse model. The efficiency of dendritoma as a weapon against tumor cells and the benefit of syngeny in experimental models are discussed.
authors.affiliation: Immunocytologie et Oncologie Moléculaire, Université de Technologie de Compiègne, Compiègne, France.
authors.forename: S
authors.initials: S
authors.lastname: Quéant
authors.forename: C-O
authors.initials: CO
authors.lastname: Sarde
authors.forename: M-G
authors.initials: MG
authors.lastname: Gobert
authors.forename: J
authors.initials: J
authors.lastname: Kadouche
authors.forename: A
authors.initials: A
authors.lastname: Roseto
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D002455:Cell Division; D003713:Dendritic Cells / Q000276:immunology*; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009101:Multiple Myeloma / Q000276:immunology* / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology
chemical_list: D019496:Cancer Vaccines