Virus attachment and replication are promoted after acquisition of host CD28 and CD152 by HIV-1.
CD28 is constitutively expressed on CD4(+) cells, but its homologue CD152 is only weakly expressed after cell activation. To determine whether these 2 costimulatory molecules can be inserted into human immunodeficiency virus type 1 (HIV-1), virus was produced in CD28- and CD152-expressing Jurkat-derived cells. Both molecules were efficiently acquired by virions. Virus attachment and infectivity were more affected by CD152 than by CD28. Given that CD28/CD152-CD80/CD86 interactions play a dominant role in antigen presentation, it can thus be proposed that the association between virus-anchored host CD28/CD152 and cell-surface CD80/CD86 on target cells might have consequences for the transmission and pathogenesis of HIV-1.
Giguere
Jean-Francois
JF
Research Center in Infectious Diseases, Laval University Hospital Research Center, and Faculty of Medicine, Laval University, Quebec, Canada.
Diou
Juliette
J
Madrenas
Joaquim
J
Tremblay
Michel J
MJ
D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000378:metabolism*; D018106:CD28 Antigens / Q000378:metabolism*; D060908:CTLA-4 Antigen; D015536:Down-Regulation; D015497:HIV-1 / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D015854:Up-Regulation; D014771:Virion / Q000378:metabolism
D015703:Antigens, CD; D000943:Antigens, Differentiation; D018106:CD28 Antigens; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human