Expression of bacterial endonucleases in Saccharomyces cerevisiae mitochondria.
Expression vectors were created in which the 5' end of the Saccharomyces cerevisiae CDC9 gene, which encodes a mitochondrial targeting peptide, was cloned in-frame with the coding regions of the EcoR I, Hind III, and Pst I endonuclease genes. Expression of the EcoR I and Hind III fusion proteins inhibited growth of yeast on glycerol-containing media and resulted in the nearly quantitative restriction digestion of their mitochondrial DNA. In contrast, expression of Pst I, which does not recognize any sites within yeast mitochondrial DNA, had no effect on growth in glycerol-containing media, and did not affect the integrity of the mitochondrial genome.
Donahue
Sarah L
SL
Department of Pharmacology, University of Minnesota Medical School, 6-120 Jackson Hall, 321 Church Street, SE, Minneapolis, MN 55455, USA.
Lakshmipathy
Uma
U
Campbell
Colin
C