Clinical and immunological responses to undiluted and diluted smallpox vaccine with vaccinia virus of Lister strain.
The potential to increase the supply of vaccine by diluting the vaccinia virus of Lister strain to face possible bioterrorism with smallpox was evaluated. Vaccinia-naïve subjects (n=97) were randomized to receive either undiluted or diluted (1:5, 1:10) vaccine, and previously vaccinated subjects (n=122) were randomized to receive either undiluted or diluted (1:10, 1:30) vaccine. Except two subjects who received 1:30 diluted vaccine, the vaccination of all subjects was successful clinically. All subjects had significant vaccinia-specific T cell and antibody responses. The diluted vaccine was not associated with decreased local or systemic reactions, lower T cell responses, or higher antibody titers when compared with undiluted vaccine. Here we show the diluted vaccine of Lister strain can be used in vaccinia-naïve subjects and previously vaccinated subjects if viral titer > or =10(8) and 10(7.5) pfu/mL after dilution, respectively. The reactogenicity of vaccinia virus may not be a dose-dependent response.
Hsieh
Szu-Min
SM
Division of Infectious Diseases, Department of Internal Medicine, National Taiwan University Hospital and College of Medicine, National Taiwan University, No. 7 Chung-Shan South Road, Taipei 100, Taiwan, ROC.
Chen
Shey-Ying
SY
Sheu
Gwo-Chang
GC
Hung
Min-Nan
MN
Chou
Wen-Hsiang
WH
Chang
Shan-Chwen
SC
Hsu
Kwo-Hsiung
KH
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D016037:Single-Blind Method; D012900:Smallpox Vaccine / Q000008:administration & dosage* / Q000009:adverse effects / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology; D014611:Vaccination; D014616:Vaccinia virus; D012901:Variola virus / Q000276:immunology*
D000914:Antibodies, Viral; D012900:Smallpox Vaccine