title: Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog.
abstract: Faithful transmission of genetic information during mitotic divisions depends on bipolar attachment of sister kinetochores to the mitotic spindle and on complete resolution of sister-chromatid cohesion immediately before the metaphase-to-anaphase transition. Separase is thought to be responsible for sister-chromatid separation, but its regulation is not completely understood. Therefore, we have screened for genetic loci that modify the aberrant phenotypes caused by overexpression of the regulatory separase complex subunits Pimples/securin and Three rows in Drosophila. An interacting gene was found to encode a constitutive centromere protein. Characterization of its centromere localization domain revealed the presence of a diverged CENPC motif. While direct evidence for an involvement of this Drosophila Cenp-C homolog in separase activation at centromeres could not be obtained, in vivo imaging clearly demonstrated that it is required for normal attachment of kinetochores to the spindle.
authors.affiliation: Department of Genetics, BZMB, University of Bayreuth, 95440 Bayreuth, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Heeger
authors.forename: Oliver
authors.initials: O
authors.lastname: Leismann
authors.forename: Ralf
authors.initials: R
authors.lastname: Schittenhelm
authors.forename: Oliver
authors.initials: O
authors.lastname: Schraidt
authors.forename: Stefan
authors.initials: S
authors.lastname: Heidmann
authors.forename: Christian F
authors.initials: CF
authors.lastname: Lehner
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics*; D002503:Centromere / Q000201:enzymology / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000235:genetics*; D004330:Drosophila / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics*; D010450:Endopeptidases / Q000737:chemistry* / Q000235:genetics*; D005124:Eye Abnormalities / Q000235:genetics; D017344:Genes, Insect; D018386:Kinetochores / Q000201:enzymology; D008938:Mitosis / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D064247:Separase; D008941:Spindle Apparatus / Q000201:enzymology / Q000235:genetics; D014921:Wings, Animal / Q000002:abnormalities / Q000254:growth & development
chemical_list: C581136:CENP-C protein, Drosophila; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; D021122:Protein Subunits; C060905:centromere protein C; D010450:Endopeptidases; D064247:Separase; C577989:Sse protein, Drosophila