[Induction of committed differentiation from yolk sac stem cells to CFU-GM].
OBJECTIVE
To set up a stable technology for the growth of yolk sac CFU-GM.


METHODS
The effects of various inductive factors on the growth of yolk sac CFU-GM and the characteristic of colonies were investigated. In vitro agar culture method was used in this study.


RESULTS
We found close relationship between the number of E7.5 - E8.5 yolk sac cells planted and the number of the formation of CFU-GM colonies. The number of the formation of CFU-GM colonies in DMEM culture system was higher than that in the IMDM culture system with the same number of yolk sac cells planted. The effect of HS culture system on the formation of CFU-GM colonies was better than that of FBS culture system. In contrast with GM-CSF, WEHI-3-CM had much more significant CFU-GM growth promoting effect (4.14 times improved, P < 0.01). The colonies derived from yolk sac cells in this study were CFU-GM colonies by in situ fixation and H. E staining.


CONCLUSIONS
The committed differentiation from yolk sac stem cells to CFU-GM is affected by many factors. The combination of DMEM, HS, GM-CSF or WEHI-3-CM in culture system is optimal for yolk sac CFU-GM growth.
Mi
Zu-Xia
ZX
Stem Cell Research Center, Hainan Medical College, Haikou 570102, China.
Xie
Qi-Yang
QY
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008264:Macrophages / Q000166:cytology; D051379:Mice; D038042:Myelopoiesis / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*; D015017:Yolk Sac / Q000166:cytology*