title: Use of fine needle aspirates and flow cytometry for the diagnosis, classification, and immunophenotyping of canine lymphomas.
abstract: Fifty canine lymphomas were classified cytomorphologically using the updated Kiel classification scheme. Aspirates of lymph nodes from dogs with lymphoma were stained using 5 canine-specific antibodies and 3 human-specific antibodies that cross-react with canine lymphocytes. The antibody-stained aspirates were analyzed by flow cytometry. A total of 32 (64%) of the 50 lymphomas were characterized as B-cell origin and 18 (36%) were of T-cell origin. B-cell lymphomas were identified in 12 females and 20 males with a mean age of 8.35 years. T-cell lymphomas were identified in 8 females and 10 males with a mean age of 7.9 years. A minority of the lymphomas were low-grade B-cell and T-cell lymphomas (6/50, 12% and 4/50, 8%, respectively). The most common morphologic types were high-grade centroblastic and unclassifiable plasmacytoid for B- and T-cell lymphomas (18/50, 36% and 7/50, 14%, respectively).
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, University of Kafkas, Pasacayiri, Kars 36100, Turkey.
authors.forename: Mahmut
authors.initials: M
authors.lastname: Sözmen
authors.forename: Silvia
authors.initials: S
authors.lastname: Tasca
authors.forename: Erika
authors.initials: E
authors.lastname: Carli
authors.forename: Davide
authors.initials: D
authors.lastname: De Lorenzi
authors.forename: Tommaso
authors.initials: T
authors.lastname: Furlanello
authors.forename: Marco
authors.initials: M
authors.lastname: Caldin
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D044963:Biopsy, Fine-Needle / Q000379:methods*; D004283:Dog Diseases / Q000145:classification / Q000175:diagnosis / Q000276:immunology; D004285:Dogs; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000662:veterinary*; D008198:Lymph Nodes / Q000473:pathology; D008223:Lymphoma / Q000145:classification / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D016393:Lymphoma, B-Cell / Q000145:classification / Q000175:diagnosis / Q000662:veterinary; D016399:Lymphoma, T-Cell / Q000145:classification / Q000175:diagnosis / Q000662:veterinary; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology