title: Separation and identification of Aconitum alkaloids and their metabolites in human urine.
abstract: To study the safety of Aconitum medicinal herbs in clinic and identify Aconitum alkaloids poisoning in forensic medicine, Aconitum alkaloids and their metabolites were separated and identified in human urine by liquid chromatography-electrospray ionization-multi-stage mass spectrometry (LC-ESI-MS(n)) and chemical pathway of metabolism was investigated. The alkaloids and their metabolites in the urine sample were extracted with solid-phase cartridges and separated by HPLC with acetonitrile-water-formic acid (40:60:0.5) mobile phase. Structures of five metabolites and three parent Aconitum alkaloids were identified with multi-stage mass spectrometry data through comparison with authentic substances as aconitine (M(1)), mesaconitine (M(2)), hypaconitine (M(3)), benzoylaconine (M(4)), benzoylmesaconine (M(5)), benzoylhypaconine (M(6)), 16-O-demethylaconitine (M(7)) and 16-O-demethylhypaconitine (M(8)), respectively. Among them, M(8) was identified and reported for the first time. Metabolic pathways of Aconitum alkaloids in human body were proposed.
authors.affiliation: College of Chemistry, Jilin University, 10 Qianwein Street, Changchun 130012, China. Zhangh4@163.com
authors.forename: Hong-Gui
authors.initials: HG
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Duan
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Chen
authors.forename: Da-Fang
authors.initials: DF
authors.lastname: Zhong
authors.forename: Han-Qi
authors.initials: HQ
authors.lastname: Zhang
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000302:isolation & purification* / Q000652:urine; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000378:metabolism; D005260:Female; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal