Cytotoxicity of a dentine bonding agent on four different cell-lines.
The aim of this study was to compare the cytotoxicity of a recently available dentine bonding agent on four different cell-lines (three human dental pulp fibroblast cell-lines and one mouse 3T3 fibroblast cell-line).


METHODS
Three human dental pulp cell-lines from 3 different donors and one established 3T3 mouse cell-line were grown and sub-cultured. Cell viability following exposure to Scothbond was then compared to a similar number of controls using the MTT assay.


RESULTS
Scotchbond 1 was cytotoxic to all four cell-lines. 3T3 cells showed a survival rate of about 60% as compared to two of the human dental pulp cells which showed a significantly lower survival rate (p<0.05, Kruskal-Wallis Multiple-Comparison Test).


CONCLUSIONS
These findings indicated that is cytotoxic to both human pulp and 3T3 cell-lines. In general, the human pulp cell-lines showed higher sensitivity than the 3T3 cell-lines.


CONCLUSIONS
Scotchbond 1 cannot be recommended for direct pulp capping techniques and care should be taken when using this dentine bonding agent in cavities where the remaining dentine layer is minimal.
Moodley
D
D
Oral and Dental Research Institute, University of the Western Cape, Faculty of Dentistry, Tygerberg, South Africa.
Grobler
S R
SR
Olivler
A
A
D016475:3T3 Cells; D000328:Adult; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D003785:Dental Pulp Capping; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D051379:Mice; D019279:Resin Cements / Q000633:toxicity*
D017220:Dentin-Bonding Agents; D019279:Resin Cements; C041330:Scotchbond