[Detection of the integration of human FIX (hFIX)on chromosomes of transgenic mice by fluorescence in situ hybridization].
Fluorescence in situ hybridization (FISH) was used to detect the integration of hFIX on chromosomes of transgenic mice from F1 to F4 generation in two strains. For transgenic mice, 98%-100% of metaphases and 85%-94% of interphases showed hybridization signal. For negative control mice,100% of metaphases and 95%-96% of interphases showed no hybridization signal. The results demonstrated that FISH developed to detect the integration sites of hFIX was high efficient and specific. The integration sites of the transgenic mice analyzed were both single but different between the two strains. The integration chromosomes can be found in the transgenic mice from F1 to F4 generation and the integration sites were the same as each of the strains,which indicated that the transgene was stably integrated and transmitted to offspring.
Xiao
Yan-Ping
YP
Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai 200040, China. ypxiao2001@yahoo.com
Xi
Ying
Y
Huang
Wen-Ying
WY
Huang
Ying
Y