Influence of condensation temperature on selected exhaled breath parameters.
BACKGROUND
The effects of changes in cooling temperature on biomarker levels in exhaled breath condensate have been little investigated. The aim of the study was to test the effect of condensation temperature on the parameters of exhaled breath condensate and the levels of selected biomarkers.


METHODS
Exhaled breath condensate was collected from 24 healthy subjects at temperatures of -10, -5, 0 and +5 C degrees. Selected parameters (condensed volume and conductivity) and biomarkers (hydrogen peroxide, malondialdehyde) were measured.


RESULTS
There was a progressive increase in hydrogen peroxide and malondialdehyde concentrations, and condensate conductivity as the cooling temperature increased; total condensate volume increased as the cooling temperature decreased.


CONCLUSIONS
The cooling temperature of exhaled breath condensate collection influenced selected biomarkers and potential normalizing factors (particularly conductivity) in different ways ex vivo. The temperature of exhaled breath condensate collection should be controlled and reported.
Goldoni
Matteo
M
National Institute of Occupational Safety and Prevention, Research Center at the University of Parma, Parma, Italy. matgold@libero.it
Caglieri
Andrea
A
Andreoli
Roberta
R
Poli
Diana
D
Manini
Paola
P
Vettori
Maria Vittoria
MV
Corradi
Massimo
M
Mutti
Antonio
A
D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D001944:Breath Tests; D006801:Humans; D008171:Lung Diseases / Q000175:diagnosis*; D015203:Reproducibility of Results; D013696:Temperature
D015415:Biomarkers