Platelet-rich plasma influence on human osteoblasts growth.
OBJECTIVE
The influence of progressively high concentrations of platelet-rich plasma (PRP) on human osteoblast hFOB1.19 proliferation was evaluated.


METHODS
The PRP was obtained from a human source. Two experiments were conducted. In the first one, PRP was diluted to 50%, 25%, 12.5% and 6.125% (v/v) with culture medium (Modified Eagle's Medium (MEM) : Ham's F12 Medium (HAM-F12%) and 1% antibiotics-antimicotic) supplemented with 10% of fetal bovine serum (FBS). In the second experiment, all conditions were identical except for the absence of FBS in the culture medium.


RESULTS
The results of the osteoblast proliferation test were higher when stimulated by the 50% PRP dilution, with or without FBS. A further study is suggested to determine if concentrations above 50% could cause higher rates of osteoblast proliferation. In this study, the results were not statistically different (P<0.05) with 12.5% and 6.125% PRP dilutions. Additionally, it was shown that FBS is not necessary for PRP-mediated induction of osteoblast proliferation.


CONCLUSIONS
This study concluded that PRP promotes osteoblast proliferation and suggested its clinical application to bone graft procedures in implant dentistry.
Ferreira
Cimara Fortes
CF
Department of Dental Implantology, University of Santa Catarina, Florianópolis, Brazil. cimarafortes@hotmail.com
Carriel Gomes
Márcia Cristina
MC
Filho
José Scarso
JS
Granjeiro
José Mauro
JM
Oliveira Simões
Cláudia Maria
CM
Magini
Ricardo de Souza
Rde S
D001792:Blood Platelets / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D004396:Coloring Agents; D003470:Culture Media; D016895:Culture Media, Serum-Free; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000502:physiology*; D010949:Plasma; D013778:Tetrazolium Salts; D013844:Thiazoles
D004396:Coloring Agents; D003470:Culture Media; D016895:Culture Media, Serum-Free; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue