Neuroprotective and free radical scavenging activities of phenolic compounds from Hovenia dulcis.
The EtOAc-soluble fraction from a methanolic extract of Hovenia dulcis Thunb. exhibited neuroprotective activity against glutamate-induced neurotoxicity in mouse hippocampal HT22 cells. The neuroprotective activity-guided isolation resulted in 8 phenolic compounds (1-8), such as vanillic acid (1), ferulic acid (2), 3,5-dihydroxystilbene (3), (+)-aromadendrin (4), methyl vanillate (5), (-)-catechin (6), 2,3,4-trihydrobenzoic acid (7), and (+)-afzelechin (8). Among these, compounds 6 and 8 had a neuroprotective effect on the glutamate-induced neurotoxicity in HT22 cells. Furthermore, compound 6 had a DPPH free radical scavenging effect with an IC50 value of 57.7 microM, and a superoxide anion radical scavenging effect with an IC50 value of 8.0 microM. Both compounds 6 and 8 had ABTS cation radical scavenging effects with IC50 values of 7.8 microM and 23.7 microM, respectively. These results suggest that compounds 6 and 8 could be neuroprotectants owing to their free radical scavenging activities.
Li
Gao
G
Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yuseong, Daejeon 305-333, Korea.
Min
Byung-Sun
BS
Zheng
Changji
C
Lee
Joongku
J
Oh
Sei-Ryang
SR
Ahn
Kyung-Seop
KS
Lee
Hyeong-Kyu
HK
D000818:Animals; D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D002392:Catechin / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D005419:Flavonoids; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D018698:Glutamic Acid; D020128:Inhibitory Concentration 50; D051379:Mice; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D010851:Picrates / Q000378:metabolism; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal; D027823:Rhamnaceae; D013451:Sulfonic Acids / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013634:Tannins / Q000302:isolation & purification / Q000494:pharmacology*
D001578:Benzopyrans; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; D013634:Tannins; D013481:Superoxides; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D018698:Glutamic Acid; D002392:Catechin; C004931:1,1-diphenyl-2-picrylhydrazyl; C510796:afzelechin