title: Determination of prevalence and risk factors of infection with Babesia in small ruminants from Greece by polymerase chain reaction amplification.
abstract: A total of 124 blood samples were collected from 92 sheep and 32 goats from 21 randomly selected herds located in two regions of Greece. Data on the characteristics of the animals (species, gender, age, tick burden, presence of haemoglobinuria, prior treatment for babesiosis) and the herd (location, size, species of animals, dogs associated with the herds, tick burden of dogs associated with the herds) were collected through questionnaires. Nineteen animals (15%) produced the DNA fragment specific for Babesia of which 16 were sheep and three were goats. Nucleotide sequence of PCR products revealed 100% homology with Babesia ovis 18S rRNA gene. Nine farms (43%) were found positive for B. ovis. The percentage of positive animals in each farm varied between 10 and 61%. The relative risk of the presence of ticks in sheep and goats (p<0.01) and farm dogs (p<0.01) for PCR-positive results for B. ovis in sheep and goats was found 6.63 and 4.14, respectively.
authors.affiliation: Department of Anatomy and Physiology of Farm Animals, Faculty of Animal Science, Agricultural University of Athens, 75 Iera Odos Street, Votanikos, 11855 Athens, Greece. gtheo@aua.gr
authors.forename: G
authors.initials: G
authors.lastname: Theodoropoulos
authors.forename: M
authors.initials: M
authors.lastname: Gazouli
authors.forename: J A
authors.initials: JA
authors.lastname: Ikonomopoulos
authors.forename: V
authors.initials: V
authors.lastname: Kantzoura
authors.forename: A
authors.initials: A
authors.lastname: Kominakis
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification; D001404:Babesiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D002417:Cattle; D016054:DNA, Protozoan / Q000097:blood; D015511:Goat Diseases / Q000453:epidemiology* / Q000469:parasitology; D006041:Goats; D006115:Greece / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D016053:RNA, Protozoan / Q000737:chemistry; D012337:RNA, Ribosomal, 18S / Q000737:chemistry; D012307:Risk Factors; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000469:parasitology; D013987:Ticks / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S