title: GATA-4:FOG interactions regulate gastric epithelial development in the mouse.
abstract: Transcription factor GATA-4 is a key participant in cytodifferentiation of the mouse hindstomach. Here we show that GATA-4 cooperates with a Friend-of-GATA (FOG) cofactor to direct gene expression in this segment of gut. Immunohistochemical staining revealed that GATA-4 and FOG-1 are co-expressed in hindstomach epithelial cells from embryonic days (E) 11.5 to 18.5. The other member of the mammalian FOG family, FOG-2, was not detected in gastric epithelium. To show that GATA-4:FOG interactions influence stomach development, we analyzed Gata4(ki/ki) mice, which express a mutant GATA-4 that cannot bind FOG cofactors. Sonic Hedgehog, an endoderm-derived signaling molecule normally down-regulated in the distal stomach, was over-expressed in hindstomach epithelium of E11.5 Gata4(ki/ki) mice, and there was a concomitant decrease in fibroblast growth factor-10 in adjacent mesenchyme. We conclude that functional interaction between GATA-4 and a member of the FOG family, presumably FOG-1, is required for proper epithelial-mesenchymal signaling in the developing stomach.
authors.affiliation: Department of Pediatrics, Washington University School of Medicine, St. Louis Children's Hospital, St. Louis, MO 63110, USA.
authors.forename: Christina M
authors.initials: CM
authors.lastname: Jacobsen
authors.forename: Susanna
authors.initials: S
authors.lastname: Mannisto
authors.forename: Susan
authors.initials: S
authors.lastname: Porter-Tinge
authors.forename: Elena
authors.initials: E
authors.lastname: Genova
authors.forename: Helka
authors.initials: H
authors.lastname: Parviainen
authors.forename: Markku
authors.initials: M
authors.lastname: Heikinheimo
authors.forename: Igor I
authors.initials: II
authors.lastname: Adameyko
authors.forename: Sergei G
authors.initials: SG
authors.lastname: Tevosian
authors.forename: David B
authors.initials: DB
authors.lastname: Wilson
mesh_terms: D000818:Animals; D015536:Down-Regulation; D004727:Endothelium / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D004848:Epithelium / Q000196:embryology* / Q000378:metabolism; D051526:Fibroblast Growth Factor 10 / Q000235:genetics / Q000502:physiology; D050991:GATA4 Transcription Factor / Q000378:metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008648:Mesoderm / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013270:Stomach / Q000196:embryology*; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: C496463:FGF10 protein, human; D051526:Fibroblast Growth Factor 10; D050991:GATA4 Transcription Factor; D053823:Hedgehog Proteins; D009687:Nuclear Proteins; C509239:SHH protein, human; D015534:Trans-Activators; D014157:Transcription Factors; C107310:Zfpm1 protein, mouse