Determination of antihypertensive small peptides, Val-Tyr and Ile-Val-Tyr, by fluorometric high-performance liquid chromatography combined with a double heart-cut column-switching technique.
A double column-switching HPLC method with naphthalene-2,3-dialdehyde (NDA) was applied for determination of two plasma antihypertensive peptides, Val-Tyr (VY) and Ile-Val-Tyr (IVY). After a first separation on a Phe-ODS column, double heart-cuts of the retention time corresponding to NDA-VY and NDA-IVY elutions were successfully separated on an analytical ODS column: 60% acetonitrile in 0.1% trifluoroacetic acid containing 5 mM sodium 1-octanesulfonate at 1.0 mL/min. Within-run coefficients of variation were 1.73% and 4.73% for VY and IVY, respectively.
Ueno
Takao
T
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Tanaka
Mitsuru
M
Matsui
Toshiro
T
Matsumoto
Kiyoshi
K
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004151:Dipeptides / Q000097:blood / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods*; D008297:Male; D009842:Oligopeptides / Q000097:blood / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
D004151:Dipeptides; D009842:Oligopeptides; C409454:isoleucyl-valyl-tyrosine; C414029:valyltyrosine