Microbial regulation of intestinal radiosensitivity.
We describe a method for treating germ-free (GF) mice with gamma-irradiation and transplanting them with normal or genetically manipulated bone marrow while maintaining their GF status. This approach revealed that GF mice are markedly resistant to lethal radiation enteritis. Furthermore, administering lethal doses of total body irradiation to GF mice produces markedly fewer apoptotic endothelial cells and lymphocytes in the mesenchymal cores of their small intestinal villi, compared with conventionally raised animals that have acquired a microbiota from birth. Analysis of GF and conventionally raised Rag1-/- mice disclosed that mature lymphocytes are not required for the development of lethal radiation enteritis or the microbiota-associated enhancement of endothelial radiosensitivity. Studies of gnotobiotic knockout mice that lack fasting-induced adipose factor (Fiaf), a fibrinogen/angiopoietin-like protein normally secreted from the small intestinal villus epithelium and suppressed by the microbiota, showed that Fiaf deficiency results in loss of resistance of villus endothelial and lymphocyte populations to radiation-induced apoptosis. Together, these findings provide insights about the cellular and molecular targets involved in microbial regulation of intestinal radiosensitivity.
Crawford
Peter A
PA
Center for Genome Sciences and Department of Molecular Biology, Washington University School of Medicine, St. Louis, MO 63108, USA.
Gordon
Jeffrey I
JI
D000075803:Angiopoietin-Like Protein 4; D042682:Angiopoietins; D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D001419:Bacteria / Q000472:pathogenicity*; D001798:Blood Proteins / Q000172:deficiency; D016026:Bone Marrow Transplantation; D042783:Endothelial Cells / Q000528:radiation effects; D004751:Enteritis / Q000209:etiology; D005720:Gamma Rays; D005856:Germ-Free Life; D018398:Homeodomain Proteins / Q000235:genetics; D007421:Intestine, Small / Q000473:pathology; D007422:Intestines / Q000382:microbiology* / Q000528:radiation effects*; D008214:Lymphocytes / Q000528:radiation effects; D051379:Mice; D018345:Mice, Knockout; D011836:Radiation Tolerance; D014916:Whole-Body Irradiation / Q000009:adverse effects
D000075803:Angiopoietin-Like Protein 4; D042682:Angiopoietins; C407088:Angptl4 protein, mouse; D001798:Blood Proteins; D018398:Homeodomain Proteins; C064658:RAG-1 protein