title: [Influence of processing rhubarb on it's anthraquinone contents].
abstract: OBJECTIVE
  To investigate the influence of processing of Radix et Rhizoma Rhei on it's five anthraquinone components, and to develop a HPLC quantatitive method for these components in Radix et Rhizoma Rhei.
  
  
  METHODS
  Chromatographic conditions are as follow: Kromasil-C18 (4.6 mmx 150 mm, 5 microm) column, ethanol -H2O-H3PO4 (85:15:0.1) as mobile phase and the detection wavelength at 254 nm.
  
  
  RESULTS
  The average recoveries are 97.9% (aleo-emodin), 97.1% (rhein), 97.6% (emodin), 97.4% (chrysophanol) and 99.1% (physcion). RSD are 1.4%, 1.1%, 0.9%, 1.1% and 2.2% correspondingly. After processing, the contents of five anthraquinone aglycones descended compared with the crude material, the descending percentage are 15.9% (aleo-emodin), 28.0% (rhein), 25.8% (emodin), 10.0% (chrysophanol) and 10.3% (physcion).
  
  
  CONCLUSIONS
  This method is simple, sensitive and repeatable. It' canbeased for quality control of Radix et Rhizoma Rhei and it's processed products.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, BeJing 100700, China. maoshujie@163.com
authors.forename: Xian-Duan
authors.initials: XD
authors.lastname: Li
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
mesh_terms: D000880:Anthraquinones / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis; D006358:Hot Temperature; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012250:Rheum / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; C008905:physcione; D004642:Emodin; C027113:chrysophanic acid; C020491:rhein