[PCR detection of transgenic elements in feed raw material].
Based on the heterogeneous genes usually used in transgenic crops, the PCR technique was performed with primers derived from CaMV 35S promoter (35S-promoter,originated from cauliflower mosaic virus), NOS terminator (nopaline synthase-terminator,derived from Agrobacterium tumefaciens), EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene, and CryIA(b) (delta-endotoxin,evolved from Bacillus thuringiensis subsp. kurstaki) gene to detect transgenic agents from feed raw materials of soybean dregs and corn gluten meal, respectively. Endogenous corn Zein (a protein extracted from corn gluten) gene, soybean Lectin (chitin-binding protein) gene and negative, positive control were applied for avoiding false results. The method established here has been successfully applied in detecting transgenic elements in imported feed raw material.
Wang
Ying
Y
Shenzhen Exit-Entry Inspection and Quarantine Bureau, Shenzhen 518100, China. wy-liza@21cn.com
Yu
Dao-Jian
DJ
Kang
Lin
L
Zhang
Gui-Ming
GM
Jin
Xian-Zhong
XZ
Yang
Wei-Dong
WD
Huang
Pei-Qing
PQ
Wu
Qiong
Q
Chen
Zhi-Nan
ZN
Chu
Cheng-Cai
CC
Cheng
Ying-Hui
YH