Ebselen prevents mitochondrial ageing due to oxidative stress: in vitro study of fish erythrocytes.
Nucleated trout erythrocytes under oxidative stress suffer DNA membrane damage and inactivation of glutathione peroxidase. In addition, oxidative damage increases with the age of the cell. In the present paper, we evaluate the effects of oxidative stress and ageing on mitochondrial functionality by means of transmission electron microscopy and cytofluorimetric determination of mitochondrial membrane potential and intracellular levels of reactive oxygen species. The protective activity of the antioxidant organoselenium compound ebselen, a mimic of glutathione peroxidase, is also evaluated. Ebselen prevents the drastic structural and functional changes in mitochondria in aged RBCs induced by oxidative stress. However, the antioxidant does not prevent swelling of the mitochondria.
Tiano
Luca
L
Dipartimento di Biologia Molecolare, Cellulare e Animale, Università degli Studi di Camerino, Via Camerini 2, I-62032, Camerino (MC), Italy. luca.tiano@unicam.it
Fedeli
Donatella
D
Santoni
Giorgio
G
Davies
Ian
I
Wakabayashi
Takashi
T
Falcioni
Giancarlo
G