title: Distribution of epithelial sodium channels and mineralocorticoid receptors in cardiovascular regulatory centers in rat brain.
abstract: Epithelial sodium channels (ENaC) are important for regulating sodium transport across epithelia. Functional studies indicate that neural mechanisms acting through mineralocorticoid receptors (MR) and sodium channels (presumably ENaC) are crucial to the development of sympathoexcitation and hypertension in experimental models of salt-sensitive hypertension. However, expression and localization of the ENaC in cardiovascular regulatory centers of the brain have not yet been studied. RT-PCR and immunohistochemistry were performed to study ENaC and MR expression at the mRNA and protein levels, respectively. Both mRNA and protein for alpha-, beta-, and gamma-ENaC subunits and MR were found to be expressed in the rat brain. All three ENaC subunits and MR were present in the supraoptic nucleus, magnocellular paraventricular nucleus, hippocampus, choroid plexus, ependyma, and brain blood vessels, suggesting the presence of multimeric channels and possible regulation by mineralocorticoids. In most cortical areas, thalamus, amygdala, and suprachiasmatic nucleus, notable expression of gamma-ENaC was undetectable, whereas alpha- and beta-ENaC were abundantly expressed pointing to the possibility of a heterogeneous population of channels. The findings suggest that stoichiometrically different populations of ENaC may be present in both epithelial and neural components in the brain, which may contribute to regulation of cerebrospinal fluid and interstitial Na+ concentration as well as neuronal excitation.
authors.affiliation: Hypertension Unit, Univ. of Ottawa Heart Institute, Ottawa, ON, Canada K1Y 4W7.
authors.forename: Md Shahrier
authors.initials: MS
authors.lastname: Amin
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Wang
authors.forename: Erona
authors.initials: E
authors.lastname: Reza
authors.forename: Stewart C
authors.initials: SC
authors.lastname: Whitman
authors.forename: Balwant S
authors.initials: BS
authors.lastname: Tuana
authors.forename: Frans H H
authors.initials: FH
authors.lastname: Leenen
mesh_terms: D000818:Animals; D017704:Baroreflex / Q000502:physiology*; D001921:Brain / Q000378:metabolism*; D053503:Epithelial Sodium Channels; D006321:Heart / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018161:Receptors, Mineralocorticoid / Q000378:metabolism*; D015222:Sodium Channels / Q000378:metabolism*; D014018:Tissue Distribution; D014882:Water-Electrolyte Balance / Q000502:physiology*
chemical_list: D053503:Epithelial Sodium Channels; D018161:Receptors, Mineralocorticoid; D015222:Sodium Channels