title: Detection and sequence analysis of DNA polymerase and major envelope protein genes in koi herpesviruses derived from Cyprinus carpio in Gunma prefecture, Japan.
abstract: Koi herpesvirus (KHV), which is believed to be an emerging virus, causes fatal diseases in carps. Since the 1990s, the presence of KHV has been confirmed in several countries. In Japan, from 2003 to 2004, large outbreaks of KHV infection in farmed carps resulted in the death of numerous fishes. From April to May 2004, we collected 43 dead or dying carps exhibiting typical symptoms of KHV infection in Gunma prefecture. To conduct a molecular epidemiologic study of KHV in our prefecture, we amplified DNA polymerase and the major envelope protein genes of KHV derived from carp gills using newly designed primers. We also performed sequence analysis of both genes of KHV. Sensitivity of our PCR method for amplification of DNA polymerase and the major envelope protein genes of KHV was 3 x 10(2) (100 fg) and 3 x 10(3) (1000 fg) copies of KHV genome, respectively. We detected both DNA polymerase and major envelope protein genes in 37 of 43 carps (86%). No mutation was found in both the genes sequenced from 11 strains, which included two foreign strains and one domestic strain. The results suggested that KHV strains derived from carps in our prefecture were closely related genetically to the other KHV strains.
authors.affiliation: Gunma Prefectural Institute of Public Health and Environmental Sciences, 378 Kamioki, Maebashi, Gunma 371-0052, Japan.
authors.forename: Taisei
authors.initials: T
authors.lastname: Ishioka
authors.forename: Masakazu
authors.initials: M
authors.lastname: Yoshizumi
authors.forename: Shotaro
authors.initials: S
authors.lastname: Izumi
authors.forename: Kyuma
authors.initials: K
authors.lastname: Suzuki
authors.forename: Hiroko
authors.initials: H
authors.lastname: Suzuki
authors.forename: Kunihisa
authors.initials: K
authors.lastname: Kozawa
authors.forename: Masanao
authors.initials: M
authors.lastname: Arai
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Nobusawa
authors.forename: Yukio
authors.initials: Y
authors.lastname: Morita
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kato
authors.forename: Toshie
authors.initials: T
authors.lastname: Hoshino
authors.forename: Takaji
authors.initials: T
authors.lastname: Iida
authors.forename: Kanae
authors.initials: K
authors.lastname: Kosuge
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kimura
mesh_terms: D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004196:Disease Outbreaks / Q000662:veterinary; D005393:Fish Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D005784:Gene Amplification; D005880:Gills / Q000821:virology; D006564:Herpesviridae / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; D004259:DNA-Directed DNA Polymerase