title: Development of pressurized hot water extraction followed by headspace solid-phase microextraction and gas chromatography-mass spectrometry for determination of ligustilides in Ligusticum chuanxiong and Angelica sinensis.
abstract: In this work, a simple, rapid, solvent-free, and low-cost method was developed for the determination of ligustilides in traditional Chinese medicines (TCMs), which was based on pressurized hot water extraction (PHWE) followed by headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The two bioactive compounds Z-ligustilide and E-ligustilide in two common TCMs, viz. Ligusticum chuanxiong and Angelica sinensis, were extracted by water at 150 degrees C and 40 bar, followed by concentration with HS-SPME and detection by GC-MS. PHWE and HS-SPME parameters were investigated and method validation (precision and recovery) was studied. It has been shown that the proposed method provides a powerful approach for quantitative analysis of ligustilides in TCMs. The method was applied to determination of ligustildes in the TCMs from different growing areas. The results indicate that PHWE-HS-SPME-GC-MS is a potential tool for TCM quality assessment.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Jie
authors.initials: J
authors.lastname: Ji
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Wang
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000032:analysis; D029971:Angelica sinensis / Q000737:chemistry*; D002845:Chromatography; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D030004:Ligusticum; D010936:Plant Extracts / Q000737:chemistry*; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C027820:ligustilide; D015107:4-Butyrolactone; C101574:chuangxiong extract