title: Isovitexin suppresses lipopolysaccharide-mediated inducible nitric oxide synthase through inhibition of NF-kappa B in mouse macrophages.
abstract: Isovitexin exhibits potent antioxidant activities. In this study, the activity of nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-activated RAW264.7 macrophages after incubation with isovitexin was investigated. Isovitexin was able to reduce the production of hydrogen peroxide induced by LPS in mouse macrophage RAW264.7 cells. The cells incubated with isovitexin had markedly reduced LPS-stimulated NO production with an IC (50) value of 58.5 microM. The expression of iNOS was also inhibited when the cells were treated with isovitexin. A transient transfection experiment showed that isovitexin suppressed the iNOS promoter and NF-kappaB-dependent transcriptional activities. It was also found to inhibit IKK kinase activity and prevent the degradation of IkappaBalpha in activated RAW264.7 cells. Additionally, Western blotting analysis revealed that isovitexin prevented the translocation of NF-kappaB from the cytoplasm to the nucleus. Our results indicate that its ROS scavenger and IKK inhibitory activities also contribute to the suppression of ROS-mediated NF-kappaB activity. These results suggest that isovitexin, a food phytochemical contained in dietary rice products, might have biological significance.
authors.affiliation: College of Medicine, Taipei Medical University, Taipei, Taiwan. cmlin@tmu.edu.tw
authors.forename: Chun-Mao
authors.initials: CM
authors.lastname: Lin
authors.forename: Sheng-Tung
authors.initials: ST
authors.lastname: Huang
authors.forename: Yu-Chih
authors.initials: YC
authors.lastname: Liang
authors.forename: Mei-Shan
authors.initials: MS
authors.lastname: Lin
authors.forename: Chwen-Ming
authors.initials: CM
authors.lastname: Shih
authors.forename: Yuan-Ching
authors.initials: YC
authors.lastname: Chang
authors.forename: Tzong-Yueh
authors.initials: TY
authors.lastname: Chen
authors.forename: Chien-Tsu
authors.initials: CT
authors.lastname: Chen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D047310:Apigenin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D005434:Flow Cytometry; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D012275:Oryza; D008517:Phytotherapy; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000975:Antioxidants; D017931:DNA Primers; D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D016328:NF-kappa B; D047310:Apigenin; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C049772:isovitexin