title: Suppression of cell attachment and protein adsorption onto amphiphilic polylactide-grafted dextran films.
abstract: To develop novel biodegradable biomedical materials, polylactide-grafted dextrans (Dex-g-PLA)s having various lengths, numbers of graft chains and sugar units were synthesized using the trimethylsilyl (TMS) protection method. To explore the possibility of using Dex-g-PLA as a biomedical soft-material, the contact angle, cell attachment and protein adsorption properties of the films prepared from these biodegradable and amphiphilic graft co-polymers were investigated. The poly-L-lactide (PLLA) film did not absorb water at all because of its high hydrophobicity, while the graft co-polymer films started immediately to swell after immersion in PBS. The percentage of water absorption at equilibrium increased with increasing sugar unit content. The receding contact angle of the Dex-g-PLA films against water was smaller than that of the PLLA film. The receding contact angle of Dex-g-PLA films against water decreased with increasing the sugar unit content. The top surface of the Dex-g-PLA film was suggested to be covered with hydrophilic Dex segments by means of annealing in water and to afford the wettable surface. Such a wettable surface led to the suppression of cell attachment and protein adsorption onto the film.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering and High Technology Research Center, Kansai University, Suita, Osaka 564-8680, Japan. touchi@ipcku.kansai-u.ac.jp
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Ouchi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kontani
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Saito
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ohya
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002460:Cell Line; D003911:Dextrans / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D011091:Polyesters / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D003911:Dextrans; D011091:Polyesters; D012709:Serum Albumin; D014867:Water; C033616:poly(lactide)