title: [Variants of exon 4 and its flanking region of LPL Gene in patients with hyperlipidemia].
abstract: To elucidate abnormalities of LPL gene in hyperlipidemia in the Chinese population in Guangdong,genomic DNA was extracted from leukocyte of 258 patients with primary hyperlipidemia.A segment of LPL gene including exon 4 and its flanking sequences was analyzed by PCR-SSCP. The PCR products with abnormal SSCP pattern were cloned and sequenced. As a C-->T transition mutation at-6 bp of intron 3 was found in two Chinese with hyperlipidemia and the mutation was not found in 252 normolipidemic controls,the C-->T transition in intron 3 may be related to hyperlipedemia.
authors.affiliation: Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, China. zhaoys2000@21cn.com
authors.forename: Ying-she
authors.initials: YS
authors.lastname: Zhao
authors.forename: Zhong-han
authors.initials: ZH
authors.lastname: Yang
authors.forename: Jian-sheng
authors.initials: JS
authors.lastname: Feng
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Jiang
authors.forename: Mei-yu
authors.initials: MY
authors.lastname: Wu
authors.forename: Tian-hong
authors.initials: TH
authors.lastname: Zhou