Purification of xylitol from fermented hemicellulosic hydrolyzate using liquid-liquid extraction and precipitation techniques.
Xylitol was produced by Candida guilliermondii by fermentation of sugarcane bagasse hemicellulosic hydrolysate. Undesirable impurities were extracted from the broth using either ethyl acetate, chloroform or dichloromethane. The best results on clarification of the broth without xylitol loss were obtained with ethyl acetate. When ethanol, acetone or tetrahydrofuran were used for precipitation of impurities, only tetrahydrofuran clarified the fermented broth, but a high xylitol loss (approximately 30%) was observed.
Mussatto
Solange I
SI
Department of Biotechnology, Faculty of Chemical Engineering of Lorena, Rodovia Itajubá-Lorena Km 74, 5-CEP, 12600-970, Lorena, SP, Brazil.
Santos
Júlio C
JC
Filho
Walter C Ricardo
WC
Silva
Silvio S
SS
D000085:Acetates / Q000737:chemistry; D000096:Acetone / Q000494:pharmacology; D001671:Biochemistry / Q000379:methods*; D001709:Biotechnology / Q000379:methods*; D011232:Chemical Precipitation; D002725:Chloroform / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D005663:Furans / Q000737:chemistry; D006868:Hydrolysis; D008752:Methylene Chloride / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D014993:Xylitol / Q000737:chemistry* / Q000302:isolation & purification*; D014994:Xylose / Q000737:chemistry
D000085:Acetates; D005663:Furans; D011134:Polysaccharides; D000096:Acetone; C018674:tetrahydrofuran; D008752:Methylene Chloride; C007650:ethyl acetate; D002725:Chloroform; C007916:hemicellulose; D014994:Xylose; D014993:Xylitol