Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil.
We report the frequency of the different diarrheagenic Escherichia coli (DEC) categories isolated from children with acute endemic diarrhea in Salvador, Bahia. The E. coli isolates were investigated by colony blot hybridization with the following genes probes: eae, EAF, bfpA, Stx1, Stx2, ST-Ih, ST-Ip, LT-I, LT-II, INV, and EAEC, as virulence markers to distinguish typical and atypical EPEC, EHEC/STEC, ETEC, EIEC, and EAEC. Seven of the eight categories of DEC were detected. The most frequently isolated was atypical EPEC (10.1%) followed by ETEC (7.5%), and EAEC (4.2%). EHEC, STEC, EIEC, and typical EPEC were each detected once. The strains of ETEC, EAEC, and atypical EPEC belonged to a wide variety of serotypes. The serotypes of the others categories were O26:H11 (EHEC), O21:H21 (STEC), O142:H34 (typical EPEC), and O:H55 (EIEC). We also present the clinical manifestations and other pathogenic species observed in children with DEC. This is the first report of EHEC and STEC in Salvador, and one of the first in Brazil.
Franzolin
Marcia Regina
MR
Laboratório Especial de Microbiologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil. mrfranzolin@butantan.gov.br
Alves
Rosely Cabette Barbosa
RC
Keller
Rogéria
R
Gomes
Tânia Aparecida Tardelli
TA
Beutin
Lothar
L
Barreto
Mauricio Lima
ML
Milroy
Craig
C
Strina
Agostino
A
Ribeiro
Hugo
H
Trabulsi
Luiz Rachid
LR
D015373:Bacterial Typing Techniques / Q000379:methods*; D001938:Brazil / Q000453:epidemiology; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D019353:Endemic Diseases; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006801:Humans; D010641:Phenotype; D015995:Prevalence