title: Annexin XI co-localises with calcyclin in proliferating cells of the embryonic mouse testis.
abstract: Mammalian sex determination relies on the expression of SRY, which triggers a tightly regulated cascade of gene expression leading to male differentiation. Many elements of this pathway remain to be identified. Here, we characterise Annexin XI (Anxa11), a gene whose major site of embryonic expression was within the undifferentiated and differentiating testis. Lower level expression was also observed in both sexes in the Müllerian and Wolffian ducts, the somitic dermamyotome, and the dorsal intermediate zone of the neural tube. Anxa11 transcripts were detected in the indifferent gonad from 10.5 days post coitum (dpc), becoming male specific as development proceeded. Expression was within the testis cords, initially in germ cells, and then in both Sertoli and germ cells. Annexin XI protein was seen in the testis cords from 12.5 dpc, localising to the cytoplasm of the Sertoli cells. Expression of calcyclin (S100a6), shown previously to interact with annexin XI in vitro, was also observed in proliferating cells of the embryonic testis, supporting a possible in vivo interaction.
authors.affiliation: Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Parkville, Australia.
authors.forename: Louise H
authors.initials: LH
authors.lastname: Williams
authors.forename: Peter J
authors.initials: PJ
authors.lastname: McClive
authors.forename: Jocelyn A
authors.initials: JA
authors.lastname: Van Den Bergen
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Sinclair
mesh_terms: D000818:Animals; D017302:Annexins / Q000096:biosynthesis* / Q000502:physiology; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000502:physiology; D049109:Cell Proliferation; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D005854:Germ Cells / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000096:biosynthesis* / Q000502:physiology; D012708:Sertoli Cells / Q000378:metabolism; D019849:Sex Determination Processes; D013737:Testis / Q000196:embryology* / Q000378:metabolism; D013997:Time Factors
chemical_list: D017302:Annexins; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489134:S100a6 protein, mouse; D012313:RNA