[Optimization of isolation of the concentrate of stem cells from the umbilical blood].
OBJECTIVE
To study correlations between body mass and height of the newborn, Apgar scale estimates, gestation time, volume of the obtained umbilical blood (UB), number of nucleated cells (NC); to compare manual and automatic modes of UB processing.


METHODS
330 procurements of UB were made, 230 (69.7%) samples were frozen. Comparison of 2 techniques of UB processing was made in 73 cases of double centrifugation with hydroxyethylstarch (HES) and 47 cases of using separator Sepax (Biosafe, Switzerland). Blood cell count before and after UB processing and number of CD34+ cells were estimated.


RESULTS
A correlation analysis was made of dependence of the volume of 102 samples of UB on the weight (r = 0.268, p < 0.01) and height of the fetus (r = 0.203, p < 0.05), estimation by Apgar scale (r = -0.092, p < 0.1) and gestation term (r = -0.003, p > 0.1); analysis of the number of NC dependence on the volume of UB (r = 0.102 p < 0.1), mass (r = 0.073 p > 0.1) and fetus height (r = 0.121 p > 0.1), gestation time (r = 0.159 p > 0.1), Apgar scale assessment (r = -0.174 p > 0.1). In manual UB management NC yield made up 71.9 +/- 6.7%, in automatic--81 +/- 8.0% (p < 0.05). Percent of erythrocytes removal was 73 +/- 5.7% and 80.5 +/- 6.1% (p < 0.05), respectively.


CONCLUSIONS
A weak correlation was found between UB volume, mass and height of the fetus. The number of NC in UB depends on none of the parameters. Automatic processing of UB provides a greater release of NC and better elimination of erythrocytes in minimal risk of contamination.
Tiumina
O V
OV
Savchenko
V G
VG
Gusarova
G I
GI
Pavlov
V V
VV
Zharkov
M N
MN
Volchkov
S E
SE
Rossiev
V A
VA
Gridasov
G N
GN
D018952:Antigens, CD34 / Q000276:immunology; D001771:Blood Banks / Q000592:standards; D001772:Blood Cell Count; D001793:Blood Preservation / Q000592:standards; D001827:Body Height; D001835:Body Weight; D002498:Centrifugation; D015925:Cryopreservation; D004900:Erythroblasts / Q000166:cytology; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology*; D020858:Tissue and Organ Harvesting / Q000379:methods*
D018952:Antigens, CD34