Separation and identification of Aconitum alkaloids and their metabolites in human urine.
To study the safety of Aconitum medicinal herbs in clinic and identify Aconitum alkaloids poisoning in forensic medicine, Aconitum alkaloids and their metabolites were separated and identified in human urine by liquid chromatography-electrospray ionization-multi-stage mass spectrometry (LC-ESI-MS(n)) and chemical pathway of metabolism was investigated. The alkaloids and their metabolites in the urine sample were extracted with solid-phase cartridges and separated by HPLC with acetonitrile-water-formic acid (40:60:0.5) mobile phase. Structures of five metabolites and three parent Aconitum alkaloids were identified with multi-stage mass spectrometry data through comparison with authentic substances as aconitine (M(1)), mesaconitine (M(2)), hypaconitine (M(3)), benzoylaconine (M(4)), benzoylmesaconine (M(5)), benzoylhypaconine (M(6)), 16-O-demethylaconitine (M(7)) and 16-O-demethylhypaconitine (M(8)), respectively. Among them, M(8) was identified and reported for the first time. Metabolic pathways of Aconitum alkaloids in human body were proposed.
Zhang
Hong-Gui
HG
College of Chemistry, Jilin University, 10 Qianwein Street, Changchun 130012, China. Zhangh4@163.com
Sun
Ying
Y
Duan
Ming-Yu
MY
Chen
Yu-Juan
YJ
Zhong
Da-Fang
DF
Zhang
Han-Qi
HQ
D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000302:isolation & purification* / Q000652:urine; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000378:metabolism; D005260:Female; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization
D000470:Alkaloids; D004365:Drugs, Chinese Herbal