[p53 gene codon 72 polymorphism and susceptibility to keloid].
OBJECTIVE
To investigate the relationship between p53 codon 72 polymorphism and susceptibility to keloid in a southern Chinese population.


METHODS
The p53 genotypes were determined by polymerase chain reaction-reverse dot blot (PCR-RDB) and DNA direct sequencing in 45 patients with keloid and 60 unrelated healthy controls.


RESULTS
The frequency of the p53 Pro allele among keloid patients was significantly higher than that among healthy controls (chi2 = 4.485, P = 0.034). The Pro/Arg and Arg/Arg genotype distribution among keloid patients was not significantly different from that among healthy controls (chi2 = 0.949, 1.346; P = 0.330, 0.246, respectively). However, the Pro/Pro genotype frequency among keloid patients was significantly higher than that among healthy controls (chi2 = 4.375, P = 0.036). The p53 Pro/Pro genotype significantly increased the risk for developing keloid, compared to the combination of Pro/Arg and Arg/Arg genotypes,with the odds ratio (OR) of 2.400 (95%CI: 1.048-5.498).


CONCLUSIONS
Determination of the p53 codon 72 genotype may be used as a stratification marker to predicate high-risk individuals for keloid.
Zhuo
Yang
Y
Department of Plastic Surgery, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, China.
Gao
Jian-hua
JH
Luo
Shen-qiu
SQ
Zeng
Wei-sen
WS
Hu
Zhi-qi
ZQ
Lu
Feng
F
Zhao
Yong-zhong
YZ
D000293:Adolescent; D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D002648:Child; D002681:China / Q000453:epidemiology; D003062:Codon; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007627:Keloid / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D055815:Young Adult
D003062:Codon; D016159:Tumor Suppressor Protein p53