[Resistance to antibiotics and histamine production at the bacteria, isolated from the stomatopharynx of the children with bronchial asthma].
1549 strains of bacteria (gram-positive and gram-negative bacteria and Candida) were isolated from the 117 children (65 with bronchial asthma and 52 healthy one) aged from 3 to 14 years old. Susceptibility of 1213 strains to 20 antibiotics was determined by disk-diffusion method. It was shown that 336 strains produce histidine decarboxylase and for 40 strains the quantity of the produced histamine was measured on the Moeller medium. Histamine-modifying activity was investigated at 32 strains in vitro by fluorometric method. Reduced colonization resistance of the pharynx of the children with bronchial asthma was shown. Spectrum of bacteria with histidine decarboxylase activity was more wide at the children with asthma. Histamine production was about 1 mcg/mL at streptococci, staphylococci and hemophilic bacteria, from 1 to 3 mcg/mL - at bacilli, corynebacteria and Candida, from 1 to 10 mcg/mL - at lactobacilli, enterobacteria, pseudomonades, acinetobacteria. Ability for partial histamine destruction in virowas demonstrated for some strains of lactobacilli, enterobacteria and pseudomonades (initial histamine concentration reduced on 19.4-35.8 per cent after 48-hour incubation).
Voropaeva
E A
EA
D000293:Adolescent; D001249:Asthma / Q000382:microbiology*; D002175:Candida / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D005260:Female; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism*; D006640:Histidine Decarboxylase / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009960:Oropharynx / Q000382:microbiology*
D006632:Histamine; D006640:Histidine Decarboxylase