[Construction of a new vector for direct cloning of PCR products].
A new method for construction of a cloning vector (T-vector) for direct ligation with PCR products was described. The T-vector derived from pUC118 in which the unique restriction site of Eam1105 I in the region of Amp(r) gene was deleted and an artificial DNA fragment flanking two Eam1105 I was introduced at the site of BamHI. The modified vector was named as pUC118E. A T-vector with 3' over hang end of a single T can be obtained via digesting of pUC118E with Eam1105I. PCR products can be easily cloned with this T-vector.
Hu
Xue-Jun
XJ
Medical College of Dalian University, Dalian 116622, China. huxuejun@mail.dlptt.ln.cn
Li
Jing-Quan
JQ
Yuan
Xiao-Dong
XD
Xu
Hong-Mei
HM
Zhao
Dong-Li
DL