Isaac-CACTA transposons: new genetic markers in maize and sorghum.
CACTA is an En/Spm transposon superfamily present in high copy number in plant genomes, and Isaac is a subfamily of the CACTA superfamily. The TIR sequence of the Isaac subfamily was used for the purpose of transposon display (TD) in maize and sorghum. The Isaac TD produced 50-80 amplified fragments, depending on the primer combination, and the amplification profile was highly polymorphic among maize inbred lines. Isaac TD-based phylogenetic clustering distinguished the maize inbred lines according to their lineages and was consistent with the results of phylogenetic reports derived from other marker techniques by others researchers. The Isaac TD profile proved to be highly reproducible with different brands of Taq DNA polymerases and thermocyclers. The Isaac TD was also applied to recombinant inbred lines to assess genetic segregation; we observed 40-50 recordable segregation markers, depending on the primer combination. These Isaac TD markers segregated mostly as dominant markers, although, in a few cases, non-parental bands were observed in the segregating populations. In addition, the Isaac TD was very successful in the amplification of sorghum accessions. Therefore, the Isaac TD may provide another useful protocol for genetic analysis in maize and sorghum.
Lee
Ju Kyong
JK
Plant Biotechnology Program, Division of Biotechnology, Kangwon National University, Chunchon, Korea.
Kwon
Soon-Jae
SJ
Park
Kyong-Cheul
KC
Kim
Nam-Soo
NS
D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D005819:Genetic Markers; D011110:Polymorphism, Genetic; D045868:Sorghum / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D017931:DNA Primers; D004251:DNA Transposable Elements; D005819:Genetic Markers