title: Loss-of-function mutations in a glutathione S-transferase suppress the prune-Killer of prune lethal interaction.
abstract: The prune gene of Drosophila melanogaster is predicted to encode a phosphodiesterase. Null alleles of prune are viable but cause an eye-color phenotype. The abnormal wing discs gene encodes a nucleoside diphosphate kinase. Killer of prune is a missense mutation in the abnormal wing discs gene. Although it has no phenotype by itself even when homozygous, Killer of prune when heterozygous causes lethality in the absence of prune gene function. A screen for suppressors of transgenic Killer of prune led to the recovery of three mutations, all of which are in the same gene. As heterozygotes these mutations are dominant suppressors of the prune-Killer of prune lethal interaction; as homozygotes these mutations cause early larval lethality and the absence of imaginal discs. These alleles are loss-of-function mutations in CG10065, a gene that is predicted to encode a protein with several zinc finger domains and glutathione S-transferase activity.
authors.affiliation: Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.
authors.forename: Elayne
authors.initials: E
authors.lastname: Provost
authors.forename: Grafton
authors.initials: G
authors.lastname: Hersperger
authors.forename: Lisa
authors.initials: L
authors.lastname: Timmons
authors.forename: Wen Qi
authors.initials: WQ
authors.lastname: Ho
authors.forename: Evelyn
authors.initials: E
authors.lastname: Hersperger
authors.forename: Rosa
authors.initials: R
authors.lastname: Alcazar
authors.forename: Allen
authors.initials: A
authors.lastname: Shearn
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005123:Eye / Q000378:metabolism; D005260:Female; D005804:Genes, Lethal / Q000502:physiology*; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009154:Mutation / Q000235:genetics*; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D013489:Suppression, Genetic; D016335:Zinc Fingers
chemical_list: D029721:Drosophila Proteins; C070996:PN protein, Drosophila; D005982:Glutathione Transferase; D009701:Nucleoside-Diphosphate Kinase; C061642:awd protein, Drosophila