[The effects of E1A gene on the proliferation and the cell cycle of human lung adenoma cell line].
OBJECTIVE
To investigate the effects of E1A gene on the proliferation rate and the cell cycle of human lung adenoma cell line.


METHODS
The characteristics of human adenoma cells transfected and untransfected with E1A gene were studied including the cell growth rate in vitro, the tumorigenicity in nude mouse, and cell cycle analyzed by flow cytometry. The level of P16, P21, P53 and cyclinB1 was assayed using Western blot.


RESULTS
The proliferation rate in vitro and the tumorigenicity in vivo of the human lung adenoma cells transfected with E1A gene (Anip973-E1A) were suppressed and cell cycle was retarded at G2/M phase. There was no significant change in the level of P16, P21 and P53, however, the level of cyclinB1 diminished remarkedly.


CONCLUSIONS
E1A gene could suppress the proliferation of human adenoma cells in vitro and in vivo. The expression level of cyclinB1, one of the key G2/M checkpoint modulators, was inhibited by E1A, resulting in retardation at G2/M phase. It may be one of the mechanisms of E1A as a tumor-suppressing gene.
Liang
L
L
Institute of Cancer Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
Qian
X
X
Ma
Y
Y
D017500:Adenovirus E1A Proteins / Q000235:genetics*; D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005814:Genes, Viral; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014162:Transfection
D017500:Adenovirus E1A Proteins