Organic dust induced inflammation--role of atopy and TLR-4 and CD14 gene polymorphisms.
BACKGROUND
Polymorphisms in the genes controlling for CD14 and TLR-4 may influence the outcome of endotoxin-induced effects.


METHODS
A study was performed on 146 workers in industries with exposure to organic dust containing endotoxin and 53 controls. Endotoxin exposure was measured at representative work sites. Inflammagenic markers, cytokines, and CD14 and TLR-4 polymorphisms were determined in blood.


RESULTS
Among workers, serum levels of esinophilic cationic protein (ECP) were lower compared to controls and the proportion of atopics was lower. Atopic persons had lower levels of IL-6 and IL-8, both among controls and exposed. IL-6 and ECP values were lower among those with TLR-4 /+896 AG and GG polymorphisms as compared to AA. Among atopic workers with CD-14(-550) polymorphism CC, IL-8 was lower.


CONCLUSIONS
The results suggest that CD14 and TLR-4 polymorphisms influence regulators of the inflammation induced by endotoxin in organic dusts. Atopic persons have a lower secretion of certain inflammatory cytokines.
Rylander
Ragnar
R
Department of Environmental Medicine, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
Michel
Olivier
O
D016022:Case-Control Studies; D004391:Dust; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000209:etiology / Q000235:genetics* / Q000503:physiopathology; D007124:Immunoenzyme Techniques; D007221:Industry; D007249:Inflammation / Q000209:etiology / Q000235:genetics* / Q000503:physiopathology; D018950:Lipopolysaccharide Receptors / Q000235:genetics* / Q000276:immunology; D008070:Lipopolysaccharides / Q000633:toxicity; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D011110:Polymorphism, Genetic; D013548:Sweden; D051197:Toll-Like Receptor 4 / Q000235:genetics*
D004391:Dust; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D051197:Toll-Like Receptor 4