[Detection of expression of heat shock protein 47 mRNA in pathological scar tissue by using real-time fluorescent quantitative RT-PCR].
OBJECTIVE
To detect the expression of heat shock protein 47 mRNA in pathological scar tissue by using real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR).


METHODS
The tissues of normal skin (n = 6), hypertrophic scar (n = 6) and keloid (n = 6) were adopted, which were diagnosed by Pathology Department. Based on fluorescent TaqMan methodology, the real-time fluorescent quantitative RT-PCR were adopted to detect the expression of heat shock protein 47 mRNA.


RESULTS
Compared with normal skin tissue (0.019 +/- 0.021) x 10(5), the expressions of heat shock protein 47 cDNA of hypertrophic scar tissue (1.233 +/- 1.039) x 10(5) and keloid tissue (1.222 +/- 0.707) x 10(5) were higher, being significant differences (P < 0.05).


CONCLUSIONS
A fluorescent quantitative method was successfully applied to detecting the expression of heat shock protein 47 mRNA. Heat shock protein 47 may play an important role in promoting the formation of pathological scar tissue.
Chen
Junjie
J
Department of Burns and Plastic Surgery, West China Hospital, Sichuan University, Chengdu Sichuan 610041, P R China. cjjemail@163.com
Cen
Ying
Y
D000293:Adolescent; D000328:Adult; D002648:Child; D017439:Cicatrix, Hypertrophic / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling; D050958:HSP47 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D007627:Keloid / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012867:Skin / Q000378:metabolism / Q000473:pathology; D055815:Young Adult
D050958:HSP47 Heat-Shock Proteins; D012333:RNA, Messenger