Involvement of PGE2 and TNF-alpha in LPS-tolerance in rat glial primary cultures.
The effect of repeated exposure of primary newborn rat brain glial cultures to the endotoxin lipopolysaccharide (LPS), a component of Escherichia coli membrane, on PGE(2) and TNF-alpha production was examined. PGE(2) production by cell cultures exposed to LPS at a dose of 5 microg/ml, 4 and 24 h after initial exposure did not differ from PGE(2) production by the same cultures 4 and 24 h after the first exposure to the same dose of LPS. TNF-alpha production was significantly lower with the second exposure as compared with the production by the same culture, exposed to the same dose of LPS, examined 4 and 24 h after the first exposure. We concluded that endotoxin tolerance in our culture model is associated with reduced secretion of TNF-alpha, caused by the initial exposure to LPS, while PGE(2) production remained unchanged.
Shemi
D
D
Department of Clinical Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Kaplanski
J
J
D000818:Animals; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone