[Expression of ornithine decarboxylase mRNA in the cortex and hippocampus of rat after transient cerebral ischemia].
OBJECTIVE
To detect the expression of ornithine decarboxylase (ODC) mRNA in the cortex and hippocampus of rat over different postischemic recirculation periods, and to explore the mechanism of increase in ODC activity after transient cerebral ischemia.


METHODS
A 10-min transient cerebral ischemia was produced in anesthetized rat by ameliorated four-vessel occlusion, and the expression of ODC mRNA was measured using comparative reverse transcription-polymerase chain reaction (RT-PCR) in the cortex and hippocampus of rats sacrificed at 2, 4, 6 and 8 h after the ischemia. The variations were studied.


RESULTS
There was no expression of ODC mRNA in the cortex and hippocampus of rats in nonischemic control groups, and there was expression of ODC mRNA in the cortex and hippocampus of rats in the ischemic experimental groups, with a significant increase between 2 h and 6 h after the ischemia (P < 0.01).


CONCLUSIONS
Increased expression of ODC mRNA could contribute to the increase in ODC activity after transient cerebral ischemia.
Wan
Xin
X
Department of Nerosurgery, Xiangya Hospital, Central South University, Changsha 410008, China. yinxj19@sina.com
Jiang
Bing
B
Liu
Yun-sheng
YS
Liu
Hong-wei
HW
Wu
Guang-yong
GY
Li
Wen-ze
WZ
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism
D012333:RNA, Messenger; D009955:Ornithine Decarboxylase