title: Neurotrophic factor receptor expression and in vitro nerve growth of geniculate ganglion neurons that supply divergent nerves.
abstract: We investigated which neurotrophic factors may contribute to the divergence of two peripheral nerves emanating from the geniculate ganglion. We compared receptor mRNA profiles of the neurons that supply the nerves, and also the growth of their neurites in response to neurotrophic factors in culture. Three mRNAs, Gfra2, TrkA, and TrkC, were differentially expressed. Only one ligand, Neurturin, promoted substantially different nerve regrowth from the nerves, and therefore may contribute to nerve divergence. Three receptor mRNAs were expressed in 100% of the neurons: TrkB, TrkB.T2 (kinase-lacking isoform), and NCAM-140. Ligands for these Trks and FRalpha-1 promoted more outgrowth than ligands for the other receptors. NT-3 and BDNF synergistically promoted outgrowth. Finally, receptors are coexpressed at random rates, arguing against the existence of neuronal subtypes defined by a combinatorial code of these receptors.
authors.affiliation: Department of Biology, Loyola University Chicago, Chicago, Ill., USA.
authors.forename: Adam
authors.initials: A
authors.lastname: Yamout
authors.forename: Andrej
authors.initials: A
authors.lastname: Spec
authors.forename: Jason
authors.initials: J
authors.lastname: Cosmano
authors.forename: Manoj
authors.initials: M
authors.lastname: Kashyap
authors.forename: M William
authors.initials: MW
authors.lastname: Rochlin
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002814:Chorda Tympani Nerve / Q000166:cytology / Q000378:metabolism; D005830:Geniculate Ganglion / Q000166:cytology / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D020932:Nerve Growth Factor / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis*
chemical_list: D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; D020932:Nerve Growth Factor