title: Comparison of NSP4 protein between group A and B human rotaviruses: detection of novel diarrhea-causing sequences in group B NSP4.
abstract: The human group B rotavirus is a causative agent of severe adult diarrhea. In this study, we analyzed the NSP4 structure of a group B rotavirus strain, CAL-1, and determined whether enterotoxin activity was present in CAL-1 NSP4. CAL-1 NSP4 was comprised of 219 amino acids which was longer than group A and C rotavirus NSP4, and the primary structures of their sequences differed considerably. However, CAL-1 NSP4 had an enterotoxin-like sequence (residues 106-127) that was only 27% identical to the enterotoxin region of NSP4 of KUN (a group A rotavirus strain) at residues 114-135. Interestingly, both of the synthetic peptides, one (residues 99-128) containing the enterotoxin-like sequence and the other (residues 191-219) containing 29 C-terminal amino acids of CAL-1 NSP4, induced diarrhea in 5.5-day-old mice, but not in 17.5-day-old mice, when administered parenterally. Thus, rotavirus "enterotoxin" sequences could be considerably divergent.
authors.affiliation: Department of Hygiene, Sapporo Medical University, Sapporo, Japan. ishino@sapmed.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Ishino
authors.forename: K
authors.initials: K
authors.lastname: Mise
authors.forename: H
authors.initials: H
authors.lastname: Takemura
authors.forename: M U
authors.initials: MU
authors.lastname: Ahmed
authors.forename: M M
authors.initials: MM
authors.lastname: Alam
authors.forename: T N
authors.initials: TN
authors.lastname: Naik
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D000833:Animals, Suckling; D003967:Diarrhea / Q000209:etiology*; D004768:Enterotoxins / Q000235:genetics*; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000502:physiology; D012401:Rotavirus / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014118:Toxins, Biological / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology
chemical_list: D004768:Enterotoxins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D010455:Peptides; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins