title: Cell proliferation of cultured human cancer cells are affected by the elevated tumor pressures that exist in vivo.
abstract: Elevated interstitial fluid pressure (IFP) is observed in most solid tumors. However, the study of the cellular processes of tumors and the development of chemotherapy are routinely studied using in vitro culture systems at atmospheric pressure. Using a new pressurized cell culture system, we investigated the influence of hydrostatic pressure on population dynamics of three primary osteosarcoma (HOS, U2OS, SaOS2) and two metastatic tumor cell lines (MCF7 breast, H1299 lung) that invade bone. Values of IFP in normal human bone and muscle, and in osteosarcoma tumors obtained during their surgical biopsy established the hydrostatic pressure range for the in vitro cell studies. The IFP values were obtained from a retrospective review of patient records. IFP from confirmed osteosarcoma was 35.9+/- 16.2 mmHg. Tumor IFP was significantly higher than muscle IFP (p < 0.001) and bone IFP (p < 0.003). The in vitro study measured the cell-line proliferation using hydrostatic pressures of 0, 20, 50 and 100 mmHg. The findings suggest that hydrostatic pressure either increases or decreases tumor proliferation rates depending on cell type. Furthermore, cell death was not associated with apoptosis.
authors.affiliation: Department of Surgery/Orthopaedic Surgery, Memorial Sloan Kettering Cancer Center, Weill Medical College of Cornell University, New York, NY 10021, USA. direstag@mskcc.org
authors.forename: Gene R
authors.initials: GR
authors.lastname: Diresta
authors.forename: Saminathan S
authors.initials: SS
authors.lastname: Nathan
authors.forename: Mark W
authors.initials: MW
authors.lastname: Manoso
authors.forename: Jorge
authors.initials: J
authors.lastname: Casas-Ganem
authors.forename: Chris
authors.initials: C
authors.lastname: Wyatt
authors.forename: Tadaheko
authors.initials: T
authors.lastname: Kubo
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Boland
authors.forename: Edward A
authors.initials: EA
authors.lastname: Athanasian
authors.forename: Jonathan
authors.initials: J
authors.lastname: Miodownik
authors.forename: Richard
authors.initials: R
authors.lastname: Gorlick
authors.forename: John H
authors.initials: JH
authors.lastname: Healey
mesh_terms: D016923:Cell Death / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D006874:Hydrostatic Pressure; D009369:Neoplasms / Q000503:physiopathology*