Solution conformations of two naturally occurring RNA nucleosides: 3-methyluridine and 3-methylpseudouridine.
The conformations of 3-methyluridine and 3-methylpseudouridine are determined using a combination of sugar proton coupling constants from 1D NMR spectra and 1D NOE difference spectroscopy. Both C2'-endo and C3'-endo conformations are observed for 3-methyluridine (59:41, 37 degrees C, D2O) and 3-methylpseudouridine (51:49, 37 degrees C, D2O). 3-Methyluridine preferentially adopts an anti conformation in solution, whereas 3-methylpseudouridine is primarily in a syn conformation. anti/syn-Relationships are deduced by 1D NOE difference spectroscopy.
Desaulniers
Jean-Paul
JP
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Chui
Helen M-P
HM
Chow
Christine S
CS
D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D011560:Pseudouridine / Q000031:analogs & derivatives* / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D013696:Temperature; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry
D012996:Solutions; D011560:Pseudouridine; C008513:3-methyluridine; D012313:RNA; C034686:3-methylpseudouridine; D014529:Uridine