A novel protein Depp, which is induced by progesterone in human endometrial stromal cells activates Elk-1 transcription factor.
Decidualization of the endometrial stromal cells (ESC), considered to be stimulated by progesterone and/or cAMP, is crucial for embryo implantation and placentation. In this study, we isolated a novel clone encoding decidual protein induced by progesterone (Depp) from a human ESC cDNA library enriched with progesterone-inducible genes. Depp mRNA was expressed in various human tissues including placenta, ovary and kidney. Increased expression of Depp was observed in endometria during mid- and late-secretory phases and 1st trimester deciduas. In vitro, Depp mRNA was induced in ESC within 30 min of progesterone treatment, which was inhibited by the antiprogestin RU486. Androgen alone also induced Depp expression. Depp increased the level of phosphorylated Erk and activated the Elk-1 transcription factor in human embryonal kidney 293 cells, suggesting that Depp modulates the effects of progesterone during decidualization and in the decidua by affecting gene expression. Elucidation of the biological function of Depp in the endometrium will facilitate our understanding of the molecular mechanisms of decidualization and placental development.
Watanabe
Hirohiko
H
Daigo Watanabe Clinic, Faculty of Medicine, Kyoto University, Kyoto, Japan.
Nonoguchi
Kohsuke
K
Sakurai
Toshiharu
T
Masuda
Tomoko
T
Itoh
Katsuhiko
K
Fujita
Jun
J
D000595:Amino Acid Sequence; D002460:Cell Line; D002478:Cells, Cultured; D018076:DNA, Complementary; D003656:Decidua / Q000166:cytology / Q000378:metabolism; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D015723:Gene Library; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D011374:Progesterone / Q000502:physiology*; D011506:Proteins / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D017154:Stromal Cells / Q000378:metabolism; D050807:ets-Domain Protein Elk-1 / Q000235:genetics / Q000378:metabolism*
C506096:DEPP1 protein, human; D018076:DNA, Complementary; C494112:ELK1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D012333:RNA, Messenger; D050807:ets-Domain Protein Elk-1; D011374:Progesterone