title: Effect of 4-hydroxynonenal on phosphatidylethanolamine containing condensed monolayer and on its interaction with apolipoprotein A-I.
abstract: 4-Hydroxynonenal (4HNE), generated during polyunsaturated fatty acid oxidation, is present in atherosclerotic lesions. As 4HNE is able to react with phosphatidylethanolamine (PE), we investigated, using AC polarography, whether it may alter the physico-chemical state of a condensed PE-containing phospholipid monolayer and its interaction with apoA-I. The stability of a phospholipid monolayer relative to potential (around the potential of zero charge) is dependent on lipid composition (PE>PC>PE/PC). ApoA-I insertion into PE/PC monolayer is easier than in PC monolayer. Pre-treatment of PE/PC monolayer by 4HNE does not alter monolayer stability, but decreases apo A-I insertion into the monolayer.
authors.affiliation: INSERM U-466, Biochemistry Department, IFR31, CHU Rangueil 1 Avenue Jean Poulhés, TSA 50032-31059, Toulouse Cedex 9, France. lecompte@cict.fr
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Lecompte
authors.forename: Jean
authors.initials: J
authors.lastname: Clavilier
authors.forename: Corinne
authors.initials: C
authors.lastname: Rolland
authors.forename: Xavier
authors.initials: X
authors.lastname: Collet
authors.forename: Anne
authors.initials: A
authors.lastname: Nègre-Salvayre
authors.forename: Robert
authors.initials: R
authors.lastname: Salvayre
mesh_terms: D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism*; D016632:Apolipoprotein A-I / Q000737:chemistry / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000447:Aldehydes; D016632:Apolipoprotein A-I; D003432:Cross-Linking Reagents; D010714:Phosphatidylethanolamines; C027576:4-hydroxy-2-nonenal