title: Tissue distribution of Aconitum alkaloids extracted from Radix aconiti preparata after oral administration to rats.
abstract: OBJECTIVE
  To develop an HPLC method for the determination of Aconitum alkaloids extracted from Radix aconiti preparata in rats.
  
  
  METHODS
  Waters 2690@996 PAD system was used. The analytical column was a Halsil 100 C18 column (250 mm x 4.6 mm ID, 5 microm). The mobile phase was water, methanol and diethyl amine at the ratio of 75:25:0.1. The flow rate was 0.9 mL.min(-1). The wavelength of the detector was 240 nm.
  
  
  RESULTS
  The linear ranges of aconitine in the heart, spleen, lung and kidney were 0.4-100 microg.mL(-1), the correlation coefficients were 0.9972, 0.9986, 0.9993 and 0.9994, respectively. The linear range of aconitine in liver was 2-200 microg.mL(-1) and the correlation coefficient was 0.9990. The linear ranges of hypaconitine in heart, liver, spleen, lung, kidney, brain and spinal cord were 5-100 microg.mL(-1), the correlation coefficients were 0.9994, 0.9997, 0.9998, 0.9984, 0.9998, 0.9998 and 0.9997, respectively. Detection limits (S/N = 3) of aconitine and hypaconitine were 0.4 microg.mL(-1). The recoveries of aconitine and hypaconitine ranged from 88.7% to 102.2% and 86.5% to 101.3%, respectively, and the RSD of precision of aconitine and hypaconitine was 10%.
  
  
  CONCLUSIONS
  It appears to be an accurate and effective method that can offer reference basis for in toxication of Radix aconiti preparata clinically.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Ma
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives / Q000378:metabolism*; D031841:Aconitum / Q000737:chemistry; D000284:Administration, Oral; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D004365:Drugs, Chinese Herbal; C058309:hypaconitine; D000157:Aconitine