Extraction of yeast lipids.
Quantitative extraction of lipids from the tissue or microorganism of choice is key to their subsequent analysis. In this chapter, we describe a simple and rapid protocol that relies on glass bead disruption in the presence of organic solvents to quantitatively extract lipids from yeast cells.
Schneiter
Roger
R
Division of Biochemistry, University of Fribourg, Switzerland.
Daum
Günther
G
D008055:Lipids / Q000302:isolation & purification*; D009172:Mycology / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D012997:Solvents; D013347:Subcellular Fractions / Q000737:chemistry
D008055:Lipids; D012997:Solvents