Synthesis of a biotinylated camptothecin derivative and determination of the binding sequence by T7 phage display technology.
A biotinylated derivative of the anti-tumor agent camptothecin (CPT) was synthesized and used in a phage display assay to identify drug-binding sequences. After three rounds of selection using C20-biotinylated CPT (CPT-20-B) as bait, a CPT-20-B-binding sequence, NSSQSARR, was identified.
Takakusagi
Yoichi
Y
Genome and Drug Discovery Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Ohta
Keisuke
K
Kuramochi
Kouji
K
Morohashi
Kengo
K
Kobayashi
Susumu
S
Sakaguchi
Kengo
K
Sugawara
Fumio
F
D000595:Amino Acid Sequence; D000970:Antineoplastic Agents; D017123:Bacteriophage T7; D001665:Binding Sites; D019921:Biotinylation; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013329:Structure-Activity Relationship
D000970:Antineoplastic Agents; D019151:Peptide Library; D010455:Peptides; D002166:Camptothecin