A high-resolution karyotype of cucumber (Cucumis sativus L. 'Winter Long') revealed by C-banding, pachytene analysis, and RAPD-aided fluorescence in situ hybridization.
Using molecular cytogenetic DNA markers, C-banding, pachytene analysis, and fluorescence in situ hybridization (FISH), a high-resolution karyotype was established in the cucumber. C-banding showed distinct hetero chromatic bands on the pericentromeric, telomeric, and intercalary regions of the chromosomes. The C-banding patterns were also consistent with the morphology of 4'-6-diamino-2-phenylindole dihydrochloride (DAPI)-stained pachytene chromosomes. Two repetitive DNA fragments, CsRP1 and CsRP2, were obtained by PCR and localized on the mitotic metaphase and meiotic pachytene chromosomes. CsRP1 was detected on the pericentromeric heterochromatic regions of all chromosomes, except chromosome 1. CsRP2 was detected on 5 (chromosomes 1, 2, 3, 4, and 7) of 7 chromosomes. All homologous chromosome pairs could be distinguished by FISH using 2 RAPD markers. This is the first report on molecular karyotyping of mitotic and meiotic spreads of cucumber.
Koo
Dal-Hoe
DH
School of Bioscience and Biotechnology, Chungnam National University, Korea.
Choi
Hae-Woon
HW
Cho
Jeongki
J
Hur
Yoonkang
Y
Bang
Jae-Wook
JW
D001483:Base Sequence; D018553:Cucumis sativus / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles; D007621:Karyotyping; D008677:Metaphase / Q000235:genetics; D008969:Molecular Sequence Data; D049471:Pachytene Stage / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012091:Repetitive Sequences, Nucleic Acid
D004275:DNA, Ribosomal; D005819:Genetic Markers; D007211:Indoles; D012341:RNA, Ribosomal, 5S; C007293:DAPI