title: The HLDA8 blind panel: findings and conclusions.
abstract: There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity.
authors.affiliation: Child Health Research Institute, Women's and Children's Hospital and Department of Paediatrics, University of Adelaide, 72 King William Rd, North Adelaide, 5006, Australia.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Swart
authors.forename: Mikhail P
authors.initials: MP
authors.lastname: Salganik
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Wand
authors.forename: Kathryn
authors.initials: K
authors.lastname: Tinckam
authors.forename: Edgar L
authors.initials: EL
authors.lastname: Milford
authors.forename: Karel
authors.initials: K
authors.lastname: Drbal
authors.forename: Pavla
authors.initials: P
authors.lastname: Angelisova
authors.forename: Vaclav
authors.initials: V
authors.lastname: Horejsi
authors.forename: Peter
authors.initials: P
authors.lastname: Macardle
authors.forename: Sheree
authors.initials: S
authors.lastname: Bailey
authors.forename: Enoc
authors.initials: E
authors.lastname: Hollemweguer
authors.forename: Greg
authors.initials: G
authors.lastname: Hodge
authors.forename: Judith
authors.initials: J
authors.lastname: Nairn
authors.forename: Debbrah
authors.initials: D
authors.lastname: Millard
authors.forename: Attila
authors.initials: A
authors.lastname: Dagdeviren
authors.forename: Geoffrey W
authors.initials: GW
authors.lastname: Dandie
authors.forename: Heddy
authors.initials: H
authors.lastname: Zola
mesh_terms: D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D015703:Antigens, CD / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D007120:Immunochemistry
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D000954:Antigens, Surface