title: [A specific human scFv against malignant melanoma selected from a large human phage antibody library].
abstract: OBJECTIVE
  To obtain the specific human scFv against malignant melanoma (MM) using phage antibody library technology.
  
  
  METHODS
  The library was panned with a certain kind of MM cells for 4 rounds. The antigen binding activities of random clones were tested by ELISA in order to select specific antibodies, which were then examined by DNA sequence analysis and immunohistochemistry.
  
  
  RESULTS
  In ELISA and immunohistochemical staining, the positive clone selected from the 80 random clones was able to bind one kind of MM cell specifically, while not able to bind other irrelavant cells or antigens, such as human's squamous carcinoma cells, keratinocytes, melanocytes, keratin, trypsinase, transferrin and mouse IgG, etc. The antibody's V(H) genes belonged to human IgG V(H)5 subgroup and the V(L) belonged to human V(kappa) subtype determined by DNA sequence analysis.
  
  
  CONCLUSIONS
  One specific human phage antibody against MM cells was obtained by phage display technique, which may be useful for further development of tumor-targeted therapies.
authors.affiliation: Department of Dermatology Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. youlonglm@sina.com
authors.forename: Miao
authors.initials: M
authors.lastname: Li
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Chun-ying
authors.initials: CY
authors.lastname: Li
authors.forename: He-hui
authors.initials: HH
authors.lastname: Yin
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Tian
authors.forename: Tian-wen
authors.initials: TW
authors.lastname: Gao
mesh_terms: D000906:Antibodies / Q000235:genetics* / Q000276:immunology*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology*; D007150:Immunohistochemistry; D008545:Melanoma / Q000276:immunology; D019151:Peptide Library; D017422:Sequence Analysis, DNA
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D007135:Immunoglobulin Variable Region; D019151:Peptide Library