Cloning of a protein arginine methyltransferase PRMT1 homologue from Schistosoma mansoni: evidence for roles in nuclear receptor signaling and RNA metabolism.
The most studied arginine methyltransferase is the type I enzyme, which catalyzes the transfer of an S-adenosyl-L-methionine to a broad spectrum of substrates, including histones, RNA-transporting proteins, and nuclear hormone receptor coactivators. We cloned a cDNA encoding a protein arginine methyltransferase in Schistosoma mansoni (SmPRMT1). SmPRMT1 is highly homologous to the vertebrate PRMT1 enzyme. In vitro methylation assays showed that SmPRMT1 recombinant protein was able to specifically methylate histone H4. Two schistosome proteins likely to be involved in RNA metabolism, SMYB1 and SmSmD3, that display a number of RGG motifs, were strongly methylated by SmPRMT1. In vitro GST pull-down assays showed that SMYB1 and SmSmD3 physically interacted with SmPRMT1. Additional GST pull-down assay suggested the occurrence of a ternary complex including SmPRMT1, SmRXR1 nuclear receptor, and the p160 (SRC-1) nuclear receptor coactivator. Together, these data suggest a mechanism by which SmPRMT1 plays a role in nuclear receptor-mediated chromatin remodeling and RNA transactions.
Mansure
José João
JJ
Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro 21941-590, Brazil.
Furtado
Daniel Rodrigues
DR
de Oliveira
Francisco Meirelles Bastos
FM
Rumjanek
Franklin David
FD
Franco
Glória Regina
GR
Fantappié
Marcelo Rosado
MR
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D019317:Evidence-Based Medicine; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D007527:Isoenzymes / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011484:Protein-Arginine N-Methyltransferases / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D034443:RNA Transport / Q000502:physiology*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D012550:Schistosoma mansoni / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology*
D007527:Isoenzymes; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins; D012313:RNA; D011484:Protein-Arginine N-Methyltransferases