Covalently anchored lipid structures on amine-enriched polystyrene.
The study of the adhesion of lipid vesicles on surfaces is of increasing interest in the field of medical implants and tissue engineering (protein-resistant surfaces), drug delivery, biosensors, and biochips. In this work, lipid coverage was developed from PEG-coated vesicles (with sizes from 100 to 300 nm) by covalently binding poly(ethylene glycol)-alpha-disteroylphosphatidylethanolamine-omega-benzotriazole carbonate (DSPE-PEG-BTC) molecules onto the surface amine groups by carbamate chemistry. Lipid surface density and the surface structure of multilamellar (MLVs) and extruded unilamellar (LUVs) vesicles deposited on three types of polystyrene (PS) well-plates were probed by fluorescence and atomic force microscopy (AFM) imaging. A significant difference in the vesicle surface coverage of PS substrates was observed with a substantial increase in lipid multilayers on the amine-enriched PS surface using both unilamellar and multilamellar vesicles.
Lunelli
Lorenzo
L
ITC-irst, Center for Scientific and Technological Research, Sommarive str. 18, 38050 Povo (Trento), Italy. lunelli@itc.it
Pasquardini
Laura
L
Pederzolli
Cecilia
C
Vanzetti
Lia
L
Anderle
Mariano
M
D000588:Amines / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*
D000588:Amines; D008055:Lipids; D008081:Liposomes; D011137:Polystyrenes; D011092:Polyethylene Glycols