[Transcription factors responsible for megakaryocyte-specific gene expression].
Megakaryocytes are the hematopoietic precursors of platelets, which play an essential role in thrombosis and hemostasis. Platelet factor 4 (PF4) is expressed exclusively in megakaryocytes and platelets and serves as a lineage-specific marker of megakaryocytic differentiation. We previously characterized a number of upstream enhancer and repressor elements and demonstrated that GATA-1 and ETS-1 are important for PF4 gene expression. Recently, we have determined the novel regulatory element termed "TME" in the PF4 promoter and identified a group of binding proteins from megakaryocytic HEL cells. Here we review the function of these proteins in PF4 gene expression and discuss megakaryocyte-specific gene expression and megakaryocytepoiesis.
Doi
Takefumi
T
Graduate School of Pharmaceutical Sciences, Osaka University, Yamadaoka, Suita, Japan. doi@phs.osaka-u.ac.jp
D000818:Animals; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics / Q000502:physiology; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D050982:GATA1 Transcription Factor / Q000502:physiology; D005786:Gene Expression Regulation / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology; D006801:Humans; D008533:Megakaryocytes / Q000502:physiology; D000075064:Myeloid Ecotropic Viral Integration Site 1 Protein; D009363:Neoplasm Proteins / Q000235:genetics / Q000502:physiology; D010978:Platelet Factor 4 / Q000235:genetics*; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D011401:Promoter Regions, Genetic; D050784:Proto-Oncogene Protein c-ets-1 / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology; D020080:Tandem Repeat Sequences; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D051720:Upstream Stimulatory Factors / Q000235:genetics / Q000502:physiology
D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins; D050982:GATA1 Transcription Factor; D018398:Homeodomain Proteins; D000075064:Myeloid Ecotropic Viral Integration Site 1 Protein; D009363:Neoplasm Proteins; D000075062:Pre-B-Cell Leukemia Transcription Factor 1; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; C493728:RUNX1 protein, human; D014157:Transcription Factors; D051720:Upstream Stimulatory Factors; C083400:PBX1 protein, human; D010978:Platelet Factor 4; D004247:DNA