Methylene blue photoinactivation abolishes West Nile virus infectivity in vivo.
The prevalence of West Nile virus (WNV) infections and associated morbidity has accelerated in recent years. Of particular concern is the recent demonstration that this virus can be transmitted by blood products and can cause severe illness and mortality in transfusion recipients. We have evaluated methylene blue (MB)+light as a safe and cost-effective means to inactivate WNV in vitro. This regimen inactivated WNV with an IC50 of 0.10 microM. Up to 10(7)pfu/ml of WNV could be inactivated by MB+light with no residual infectivity. MB+light inactivated three primary WNV isolates from the years 1999, 2002 and 2003 and prevented mortality in a murine model for WNV infection. Since MB is already approved for human use at a dose of 100mg/kg/day, we conjecture that MB+light treatment of blood products for high-risk patients will be efficacious and suitable for use in resource-limited settings.
Papin
James F
JF
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, USA.
Floyd
Robert A
RA
Dittmer
Dirk P
DP
D000818:Animals; D001769:Blood / Q000821:virology; D001803:Blood Transfusion; D006801:Humans; D008027:Light; D008751:Methylene Blue / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012367:RNA, Viral / Q000187:drug effects / Q000528:radiation effects; D013242:Sterilization; D010948:Viral Plaque Assay; D014901:West Nile Fever / Q000821:virology; D014902:West Nile virus / Q000187:drug effects* / Q000528:radiation effects*
D012367:RNA, Viral; D008751:Methylene Blue