1H-13C polarization transfer in membranes: a tool for probing lipid dynamics and the effect of cholesterol.
Phospholipid bilayers with over 20% cholesterol can form a liquid-ordered (l(o)) phase, which can be found in lateral domains, called rafts, in biomembranes. We show here that high-resolution (13)C and (1)H solid-state NMR are well suited to explore this phase, intermediate between gel and fluid. This approach can be applied to artificial or natural membranes, with no isotopic enrichment and with the help of magic-angle spinning (MAS), taking advantage of the high resolution and sensitivity of these nuclei. The sensitivity of magnetization transfer schemes to different lipid states has allowed us here to discriminate between various phases. We show that the phase composed of unsaturated phospholipids and cholesterol differs, in terms of lipid dynamics, both from the previously described l(o) phase and from the liquid-disordered phase.
Warschawski
Dror E
DE
UMR 7099, CNRS, Institut de Biologie Physico-Chimique, Paris, France. Dror.Warschawski@ibpc.fr
Devaux
Philippe F
PF
D002784:Cholesterol / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry*; D013696:Temperature
D008051:Lipid Bilayers; D010743:Phospholipids; D002784:Cholesterol