title: A new dendritic cell vaccine generated with interleukin-3 and interferon-beta induces CD8+ T cell responses against NA17-A2 tumor peptide in melanoma patients.
abstract: Dendritic cells derived from monocytes cultured in the presence of type I interferon were found to induce efficient T cell responses against tumor antigens in vitro. We vaccinated eight stage III or IV melanoma patients with dendritic cells generated with interferon-beta and interleukin-3, activated by poly I: C, and pulsed with the tumor-specific antigen NA17.A2. This dendritic cell vaccine was well-tolerated with only minor and transient flu-like symptoms and inflammatory reactions at the injection sites. In most patients, isotopic imaging documented dendritic cells (DC) migration from the intradermal injection site to the draining lymph nodes. Finally, mixed lymphocyte-peptide culture under limiting dilution conditions followed by tetramer labeling indicated that three out of eight patients mounted a CD8 T cell response against the NA17.A2 antigenic peptide. We conclude that DC generated in type I-IFN represent an interesting alternative to DC generated in IL-4 and GM-CSF for cancer immunotherapy.
authors.affiliation: Department of Medical Oncology, Erasme Hospital, Université Libre de Bruxelles, Route de Lennik 808, 1070, Brussels, Belgium.
authors.forename: Myrto
authors.initials: M
authors.lastname: Trakatelli
authors.forename: Michel
authors.initials: M
authors.lastname: Toungouz
authors.forename: Didier
authors.initials: D
authors.lastname: Blocklet
authors.forename: Ygierne
authors.initials: Y
authors.lastname: Dodoo
authors.forename: Laurence
authors.initials: L
authors.lastname: Gordower
authors.forename: Marianne
authors.initials: M
authors.lastname: Laporte
authors.forename: Pierre
authors.initials: P
authors.lastname: Vereecken
authors.forename: Francois
authors.initials: F
authors.lastname: Sales
authors.forename: Laurent
authors.initials: L
authors.lastname: Mortier
authors.forename: Naima
authors.initials: N
authors.lastname: Mazouz
authors.forename: Micheline
authors.initials: M
authors.lastname: Lambermont
authors.forename: Serge
authors.initials: S
authors.lastname: Goldman
authors.forename: Pierre
authors.initials: P
authors.lastname: Coulie
authors.forename: Michel
authors.initials: M
authors.lastname: Goldman
authors.forename: Thierry
authors.initials: T
authors.lastname: Velu
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes; D019496:Cancer Vaccines / Q000276:immunology*; D018929:Cell Culture Techniques; D002465:Cell Movement; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D016899:Interferon-beta / Q000276:immunology; D007377:Interleukin-3 / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D008545:Melanoma / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D008875:Middle Aged; D012878:Skin Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy*
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D007377:Interleukin-3; D016899:Interferon-beta