[Effects of celecoxib on the proliferation and apoptosis of human colorectal cancer cell line HT-29].
OBJECTIVE
To determine the effect of Celecoxib on the proliferation and apoptosis of human colorectal cancer cell line HT-29 and the probable mechanism involved.


METHODS
Using MTT assay, flow cytometry, Acridine orange and Ethidium bromide staining, and Western blotting analysis, the effects of celecoxib on the proliferation and apoptosis of HT-29 cells as well as its related mechanism were studied.


RESULTS
The growth of HT-29 cell was inhibited by celecoxib in a dose- and time-dependent manner. Sub-G1 peak was detected by FCM, and apoptotic rate was between (7.31 +/- 2.37) % and (48.30 +/- 2.86) %. The cell ratio of G0/G1 phase increased,whereas the cell ratio of S and G2/M phases decreased in a dose-dependent manner after the treatment. The HT-29 cell line exhibited some morphologic features of apoptosis, including cell shrinkage, nuclear condensation, DNA fragmentation, and formation of apoptosis bodies, and the apoptotic rate was in a dose- and time-dependent manner. Celecoxib down-regulated the expression of CDK2, CDK4 and up-regulated the expression of P2 WAF1/CIP1


CONCLUSIONS
The effect of celecoxib inhibiting cell HT-29 proliferation and inducing cell apoptosis may relate to its blocking cell cycle progress.
Peng
Jie
J
Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhang
Gui-Ying
GY
Xiao
Zhi-Qiang
ZQ
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000068579:Celecoxib; D049109:Cell Proliferation / Q000187:drug effects*; D051358:Cyclin-Dependent Kinase 4 / Q000096:biosynthesis; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000096:biosynthesis; D019073:HT29 Cells; D006801:Humans; D011720:Pyrazoles / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D050759:Cyclin-Dependent Kinase Inhibitor p21; D011720:Pyrazoles; D013449:Sulfonamides; D051358:Cyclin-Dependent Kinase 4; D000068579:Celecoxib