Enhancement of humoral and cellular immune responses by an oral Salmonella choleraesuis vaccine expressing porcine prothymosin alpha.
Previously, we showed that murine prothymosin alpha (ProT) enhances the efficacy of a pseudorabies DNA vaccine delivered by bacterial vectors. In this study, we cloned and sequenced the cDNA for porcine ProT. The deduced amino acid sequence of porcine ProT exhibited high homology to ProT from other mammals. Oral Salmonella choleraesuis vaccine carrying the ProT eukaryotic expression plasmid protected mice against virulent S. choleraesuis challenge. The adjuvant effect of ProT on humoral and cellular immune responses enhanced protective efficacy of the vaccine. Furthermore, both humoral and cellular immune responses played roles in the protective immune responses induced by the vaccine. Collectively, our results show that delivery of the ProT gene carried by attenuated S. choleraesuis augmented the immunogenicity of oral S. choleraesuis vaccine.
Shiau
Ai-Li
AL
Department of Microbiology and Immunology, National Cheng Kung University Medical College, Tainan 701, Taiwan.
Chen
Chung-Cheng
CC
Yo
Yi-Te
YT
Chu
Chun-Yen
CY
Wang
Shiang-Yiu
SY
Wu
Chao-Liang
CL
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000235:genetics; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000914:Antibodies, Viral / Q000096:biosynthesis; D000917:Antibody Formation / Q000187:drug effects*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects; D051379:Mice; D011498:Protein Precursors / Q000008:administration & dosage* / Q000235:genetics; D012475:Salmonella / Q000235:genetics / Q000276:immunology*; D012480:Salmonella Infections / Q000276:immunology / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control; D013552:Swine; D013947:Thymosin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000235:genetics; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D001428:Bacterial Vaccines; D011498:Protein Precursors; D019444:Vaccines, DNA; C040782:prothymosin alpha; D013947:Thymosin