Molecular cloning of canine nm23 cDNAs and their expression in normal and tumor tissues.
Two canine nm-23 cDNAs, designated as nm23-C1 and -C2, were isolated and characterized. Both have a putative open reading frame consisting of 459-bp encoding 152 amino acids and are highly similar to human, mouse and rat homologues. To understand the potential role of nm23-C1 and -C2 in the development of mammary gland tumors (MGT), we analyzed the mRNA expression in 14 MGT samples by RT-PCR. The samples were divided into categories according to their histopathology (benign/malignant) and metastasis. No significant difference in the mRNA expression levels of either nm23-C1 or -C2 were observed between the benign and malignant groups or the metastatic and non-metastatic groups. These results suggest that nm23-C1 and -C2 are not related to the establishment of malignancy and metastatic lesions in canine MGT cases.
Takahashi
Masashi
M
Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Kagoshima University, Japan.
Une
Ryoma
R
Fukushima
Kenjiro
K
Fujiki
Makoto
M
Misumi
Kazuhiro
K
Miyoshi
Noriaki
N
Endo
Yasuyuki
Y
Ohishi
Akihiro
A
Akuzawa
Masao
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004283:Dog Diseases / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D015870:Gene Expression; D015674:Mammary Neoplasms, Animal / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D054778:NM23 Nucleoside Diphosphate Kinases; D012333:RNA, Messenger; C517938:NME1 protein, human; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase