Influence of UV-irradiation on enzymes in mouse ocular lens: in vitro studies.
OBJECTIVE
In vitro study of the enzymes involved in aerobic, anaerobic and hexose monophosphate shunt in ultraviolet radiation exposed mice lenses.


METHODS
Of the selected enzymes, lactic dehydrogenase (LDH) was representative of anaerobic glucose oxidation, succinic dehydrogenase (SDH) of the aerobic oxidation, and Glucose-6-phosphate dehydrogenase (G-6-PDH) of the Hexose Monophosphate (HMP) shunt. Other enzymes studied were ATPase and glutathione reductase (GR).


RESULTS
Experiments with mice lenses in vitro showed that transparent lens became opaque following UV-irradiation at 360 nm. Opacification of the lens was accompanied by a change in enzyme activities for energy metabolism.


CONCLUSIONS
These changes were progressive in a manner analogous to sequential morphological changes, which would be crucial in maintaining lens transparency.
Jain
N K
NK
Department of Zoology, School of Sciences, Gujarat University, Ahmedabad, India.
Rawal
U M
UM
D000818:Animals; D002386:Cataract / Q000201:enzymology / Q000209:etiology; D004734:Energy Metabolism; D004798:Enzymes / Q000378:metabolism; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000201:enzymology* / Q000528:radiation effects*; D051379:Mice; D014466:Ultraviolet Rays / Q000009:adverse effects*
D004798:Enzymes