Effect of oral glutamine administration on bacterial tanslocation, endotoxemia, liver and ileal morphology, and apoptosis in rats with obstructive jaundice.
Postoperative complications in patients with obstructive jaundice remain increased when associated with endotoxemia and the inflammatory response due to gut barrier failure. Administration of glutamine has been proposed to maintain the integrity of the gut mucosa and thus reduce bacterial translocation (BT), but the effects of this pretreatment on apoptosis and histologic morphology of various organs affected by BT in obstructive jaundice have not been studied. We therefore studied the effects of oral glutamine supplementation on endotoxemia, BT, liver and terminal ileal morphology, and apoptosis in an experimental model of obstructive jaundice. A total of 60 male Wistar rats were randomly divided into four groups of 15 each: I, controls; II, sham-operated; III, bile duct ligation (BDL); IV, BDL + glutamine (4.5 g/kg/day in drinking water). Ileal samples for histology, DNA and protein content, liver biopsies, mesenteric lymph nodes (MLNs) for culture, and portal and systemic blood samples for endotoxin measurements were obtained 10 days later. Compared to the controls, a significant increase in contaminated MLN and liver samples and increased endotoxemia were noted in group III (p < 0.01) but were significantly reduced in group IV (p < 0.05). Group IV also had a significantly higher number of mitoses per crypt (M/c) (p < 0.05), less apoptotic body counts (ABCs) (p < 0.05), and a higher DNA content than did group III (p < 0.05). Liver biopsies from group III displayed typical changes of large duct obstruction that significantly improved after glutamine treatment, with decreased ductular proliferation. We concluded that supplementation of oral glutamine in the presence of obstructive jaundice ameliorates BT, endotoxemia, and apoptosis and improves the ileal and liver histology.
Margaritis
Vassilios G
VG
Department of Gastroenterology, University of Patras, School of Medicine, Patras 26500, Greece.
Filos
Kriton S
KS
Michalaki
Marina A
MA
Scopa
Chrisoula D
CD
Spiliopoulou
Iris
I
Nikolopoulou
Vassiliki N
VN
Vagianos
Constantine E
CE
D000284:Administration, Oral; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D018988:Bacterial Translocation / Q000187:drug effects*; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000188:drug therapy* / Q000209:etiology; D005765:Gastrointestinal Agents / Q000008:administration & dosage*; D005973:Glutamine / Q000008:administration & dosage*; D007082:Ileum / Q000187:drug effects / Q000473:pathology; D041781:Jaundice, Obstructive / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005765:Gastrointestinal Agents; D005973:Glutamine