Effect of ultraviolet B (302 nm) irradiation on viability, metabolic and detoxification functions of goat hepatocytes--in vitro study.
The object of the present study was to investigate the effect(s) of UV-B irradiation on the functional integrity, metabolic and detoxifying capacity of the isolated goat hepatocytes. Isolated goat hepatocytes were subjected to UV-B irradiation invitro for 0, 250, 500, 1250, 2500 and 7500 Joules/m2 which correspond to the irradiation time of 0, 1, 2, 5, 10 and 30 min. Cells were then analysed for Viability (Trypan blue exclusion test [TBE], 3-[4,5-dimethylthiozol-2yl]-2,5-diphenyltetrazolium bromide [MTT] assay, Membrane integrity (Lactate dehydrogenase [LDH] leakage, Lipid peroxidation) Detoxification (Ureagenesis, Cytochrome P450 activity [CYP450, Diazepam metabolism] and Glutathione-S-Transferase [GST] activity. The results show that there was no difference in functional, metabolic as well as detoxifying parameters of the hepatocytes when irradiated from 0-1250 Joules/m2, whereas a significant alteration was appreciable in the parameters such as LDH leakage, lipid peroxidation, and CYP450 activity when irradiated beyond 1250 Joules/m2. Our present findings suggest that the biologically compatible and feasible dose of UV-B irradiation for xenotransplantation appears to be 1250 Joules/m2.
Shakeel
Naseem Begum
NB
Owaisi Hospital and Research Centre, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad, 500 058, India.
Venkateshan
Vijayalakshmi
V
Parveen
Capoor
Adarsh K
AK
Habeeb
Mohammed Aejaz
MA
Khan
Ansar Ali
AA
Muzeeb
Syed
S
Mamidi
N V S Rao
NV
Khan
Aleem Ahmed
AA
Habibullah
Chittoor Mohammed
CM
D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D006041:Goats; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects* / Q000637:transplantation; D066298:In Vitro Techniques; D008658:Inactivation, Metabolic / Q000528:radiation effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000528:radiation effects; D014183:Transplantation, Heterologous; D014466:Ultraviolet Rays
D003577:Cytochrome P-450 Enzyme System; D007770:L-Lactate Dehydrogenase