[Role of eosinophils in allergic inflammation].
Eosinophils are one of the cells that play a critical role in the pathogenesis of allergic diseases. The increase in the number of eosinophils in such diseases is regulated by interleukin-5 (IL-5). The author have prepared recombinant rat IL-5 using a baculovirus expression system and examined its biological activities in rat eosinophils. It was demonstrated that recombinant rat IL-5 prolongs the survival of mature eosinophils and differentiates immature eosinophils into mature eosinophils, suggesting that rat IL-5 is a factor for eosinophilia in rats. Recombinant rat eosinophil-associated ribonuclease (Ear)-1 and Ear-2 were also prepared. Eosinophil granule proteins are thought to cause tissue damage due to their cytotoxic activity, but using recombinant rat Ear-1 and Ear-2, it was found that rat Ear-1 and Ear-2 have strong RNase A activity and bactericidal activity, suggesting that these proteins play critical roles in host defense. Finally, the important role of acetylation of histones was clarified in the differentiation of HL-60 clone 15 cells into eosinophils using the histone deacetylase inhibitors sodium n-butyrate, apicidin, and trichostatin A. These findings would be useful for further investigations of the role of eosinophils in allergic inflammation.
Ishihara
Kenji
K
Laboratory of Pathophysiological Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki Aoba, Sendai, Japan. ishihara@mail.pharm.tohoku.ac.jp
D000107:Acetylation; D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D004791:Enzyme Inhibitors / Q000494:pharmacology; D047150:Eosinophil Cationic Protein / Q000502:physiology; D047091:Eosinophil Granule Proteins / Q000502:physiology; D004804:Eosinophils / Q000166:cytology / Q000502:physiology*; D018922:HL-60 Cells / Q000166:cytology; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D006967:Hypersensitivity / Q000209:etiology*; D007249:Inflammation / Q000209:etiology*; D015848:Interleukin-5 / Q000502:physiology; D010456:Peptides, Cyclic / Q000494:pharmacology; D051381:Rats; D011994:Recombinant Proteins
D002087:Butyrates; D004791:Enzyme Inhibitors; D047091:Eosinophil Granule Proteins; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; D015848:Interleukin-5; D010456:Peptides, Cyclic; D011994:Recombinant Proteins; C102351:apicidin; C012589:trichostatin A; D047150:Eosinophil Cationic Protein