title: Variation in rDNA locus number and position among legume species and detection of 2 linked rDNA loci in the model Medicago truncatula by FISH.
abstract: Within Fabaceae, legume species have a variable genome size, chromosome number, and ploidy level. The genome distribution of ribosomal genes, easily detectable by fluorescent in situ hybridization (FISH), is a good tool for anchoring physical and genetic comparative maps. The organisation of 45S rDNA and 5S loci was analysed by FISH in the 4 closely related species: Pisum sativum, Medicago truncatula, Medicago sativa (2 diploid taxa), and Lathyrus sativus. The 2 types of rDNA arrays displayed interspecific variation in locus number and location, but little intraspecific variation was detected. In the model legume, M. truncatula, the presence of 2 adjacent 45S rDNA loci was demonstrated, and the location of the rDNA loci was independent of the general evolution of the genome DNA. The different parameters relative to clustering of the rDNA loci in specific chromosome regions and the possible basis of rDNA instability are discussed.
authors.affiliation: Institut national de la recherche agronomique (INRA), Unité de recherche sur les legumineuses (URLEG), Dijon, France. mona@epoisses.inra.fr
authors.forename: Mona
authors.initials: M
authors.lastname: Abirached-Darmency
authors.forename: Emilce
authors.initials: E
authors.lastname: Prado-Vivant
authors.forename: Liudmila
authors.initials: L
authors.lastname: Chelysheva
authors.forename: Thomas
authors.initials: T
authors.lastname: Pouthier
mesh_terms: D004275:DNA, Ribosomal / Q000235:genetics*; D018628:Gene Dosage; D008040:Genetic Linkage; D014644:Genetic Variation; D017404:In Situ Hybridization, Fluorescence; D046913:Medicago truncatula / Q000235:genetics*; D008957:Models, Genetic; D018532:Pisum sativum / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal