title: Is mitotic chromatid segregation random?
abstract: The question of whether mitotic segregation of chromatids is random or programmed assumes great significance for cellular differentiation if one recognizes that sister chromatids may have epigenetic differences and carry them from one generation into the next. The literature was examined for evidence of non-random chromosomal and chromatid segregation. Many organisms were described as undergoing non-random homologue segregation in meiosis I. The explanations for these phenomena were attributed in some instances, to peculiarities of the meiotic spindle, though in some convincing experiments, the epigenetic heterochromatin of the kinetochores was implicated. The few existing descriptions of non-random mitotic segregation were also described. Existing literature on ultrastructural, immunohistochemical, and physiological features of the chromatid kinetochores during the mitotic process was searched for evidence of asymmetry or structural differences between sister chromatids, which is presented. Also reported are descriptions of how epigenetic changes and cell differentiation can influence centromeric function and ultimately, kinetochore function. Fundamental to the hypothesis of gene regulation presented here, is the assumption that genetic foci on different chromosomes interact, and must be proximate to each other and stereologically compatible for interactions to occur. Also described are spatial changes in chromosomal territories associated with function and differentiation. These territories can be in varying nuclear locations depending on gene function, and may show asymmetry between daughter cells. Despite evidence presented for the possibility of non-random chromatid segregation at mitosis, this question will remain unanswered until the matter is specifically addressed by experiment.
authors.affiliation: Division of Anatomical Pathology, Department of Laboratory Medicine, St.Michael's Hospital, Toronto, Canada. dbell@smh.toronto.on.ca
authors.forename: C D
authors.initials: CD
authors.lastname: Bell
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002842:Chromatids / Q000235:genetics*; D020090:Chromosome Segregation / Q000235:genetics*; D006801:Humans; D007399:Interphase / Q000235:genetics; D008938:Mitosis / Q000235:genetics*