title: Gastric cancer risk in a Mexican population: role of Helicobacter pylori CagA positive infection and polymorphisms in interleukin-1 and -10 genes.
abstract: Several polymorphisms of the IL1B and IL10 gene promoters have been reported to be associated with gastric cancer risk in Caucasians. However, studies in other populations have shown differing results. We aimed to test for associations between polymorphisms in IL1B (-31 and +3954), IL10-592 and IL1RN variable number of tandem repeats (VNTR) and risk of gastric cancer in a Mexican population. DNA was extracted from sera of 183 gastric adenocarcinoma patients and 377 controls. The IL1B-31, IL1B+3954 and IL10-592 biallelic polymorphisms were discriminated using 5' Nuclease (TaqMan) assays and Pyrosequencing. The IL1RN penta-allelic VNTR polymorphism was genotyped using PCR followed by GeneScan analysis. A significant interaction was found between IL1B-31 and CagA status for the risk of intestinal-type gastric cancer (p = 0.023). Among CagA positive subjects, those with IL1B-31CC genotype had an increased risk of intestinal-type gastric cancer (OR 3.19, 95%CI = 1.05-9.68), compared to carriers of IL1B-31TT genotype. In contrast, among CagA negative subjects, no significant association of IL1B-31CC genotype with gastric cancer was observed. The IL10-592CC genotype was associated with more than doubling of the risk of the intestinal-type gastric cancer (OR, 2.20, 95%CI = 1.04-4.65). A nonsignificantly increased risk for intestinal-type gastric cancer was found in IL1RN*2 carriers (OR 1.49, 95%CI = 0.89-2.50). None of these polymorphisms was significantly related to the risk of diffuse-type gastric cancer. No significant association was found between risk of gastric cancer and the IL1B+3954 polymorphism. Individuals carrying 2 or more of the risk-associated alleles (IL1B-31C, IL1RN *2 and IL10-592C) were at increased risk for intestinal-type gastric cancer, compared to those with 0 or 1 risk-associated allele. The risk from multiple risk-associated alleles was especially high in subjects infected with CagA positive H. pylori. Our results support the identification of the IL1B-31 promoter polymorphism as a useful marker for risk of intestinal type gastric cancer in persons with CagA positive H. pylori infections.
authors.affiliation: Department of Pathology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA. lsicin@lsuhsc.edu
authors.forename: Liviu A
authors.initials: LA
authors.lastname: Sicinschi
authors.forename: Lizbeth
authors.initials: L
authors.lastname: Lopez-Carrillo
authors.forename: M Constanza
authors.initials: MC
authors.lastname: Camargo
authors.forename: Pelayo
authors.initials: P
authors.lastname: Correa
authors.forename: Rosa A
authors.initials: RA
authors.lastname: Sierra
authors.forename: Robin R
authors.initials: RR
authors.lastname: Henry
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.forename: Jovanny
authors.initials: J
authors.lastname: Zabaleta
authors.forename: Maria B
authors.initials: MB
authors.lastname: Piazuelo
authors.forename: Barbara G
authors.initials: BG
authors.lastname: Schneider
mesh_terms: D000230:Adenocarcinoma / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D016481:Helicobacter Infections / Q000235:genetics* / Q000382:microbiology; D016480:Helicobacter pylori / Q000472:pathogenicity; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017472:Receptors, Interleukin-1 / Q000235:genetics; D012307:Risk Factors; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D020080:Tandem Repeat Sequences
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; C080866:cagA protein, Helicobacter pylori; D016753:Interleukin-10