title: [Determination of ligustrazine in human plasma by high performance liquid chromatography with solid-phase extraction].
abstract: A high performance liquid chromatographic method was developed for the determination of ligustrazine in human plasma. The chromatographic separation was performed on a Luna C18 column (150 mm x 4.6 mm i.d., 5 microm) at column temperature of 40 degrees C. The mobile phase, a mixture of methanol-acetonitrile-acetate buffer of pH 5.0 (50:8:42, v/v), was delivered at a flow rate of 1.0 mL/min. The detection wavelength was 280 nm. Plasma samples were prepared with a C8 solid-phase extraction column. Linearity was confirmed in the mass concentration range of 25-5000 microg/L with the correlation coefficient of 0.9999. The extraction recovery of ligustrazine ranged from 96.72% to 100.90%. The relative standard deviations (RSDs) of intra- and inter-day assay at the mass concentrations of 50, 500 and 3000 microg/L were less than 8.64% and the accuracies were between 99.59%-103.26%. The limit of detection (LOD) was 10 microg/L. The results of this method validation satisfactorily meet the acceptance criteria of bioanalysis and the method is applicable to the pharmacokinetic studies of ligustrazine in human beings.
authors.affiliation: School of Pharmacy, Fudan University, Shanghai 200032, China. wyweng@shmu.edu.cn
authors.forename: Weiyu
authors.initials: W
authors.lastname: Weng
authors.forename: Teng
authors.initials: T
authors.lastname: Shen
authors.forename: Huinan
authors.initials: H
authors.lastname: Xu
authors.forename: Jianfang
authors.initials: J
authors.lastname: Zhang
authors.forename: Jianming
authors.initials: J
authors.lastname: Huang
authors.forename: Zhaochang
authors.initials: Z
authors.lastname: Huang
authors.forename: Jia
authors.initials: J
authors.lastname: Cai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011719:Pyrazines / Q000097:blood*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D011719:Pyrazines; C017953:tetramethylpyrazine