Chemokine-like factor 1 is a functional ligand for CC chemokine receptor 4 (CCR4).
Chemokine-like factor 1 (CKLF1) exhibits chemotactic effects on leukocytes. Its amino acid sequence shares similarity with those of TARC/CCL17 and MDC/CCL22, the cognate ligands for CCR4. The chemotactic effects of CKLF1 for CCR4-transfected cells could be desensitized by TARC/CCL17 and markedly inhibited by PTX. CKLF1 induced a calcium flux in CCR4-transfected cells and fully desensitized a subsequent response to TARC/CCL17, and TARC/CCL17 could partly desensitize the response to CKLF1. CKLF1 caused significant receptor internalization in pCCR4-EGFP transfected cells. Taken together, CKLF1 is a novel functional ligand for CCR4.
Wang
Ying
Y
Center for Human Disease Genomics, Peking University, Beijing, PR China.
Zhang
Yingmei
Y
Yang
Xue
X
Han
Wenling
W
Liu
Yanan
Y
Xu
Qianmei
Q
Zhao
Rui
R
Di
Chunhui
C
Song
Quansheng
Q
Ma
Dalong
D
D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D054414:Chemokine CCL17; D018925:Chemokines / Q000494:pharmacology*; D019742:Chemokines, CC / Q000494:pharmacology; D002633:Chemotaxis; D006801:Humans; D008024:Ligands; D062792:MARVEL Domain-Containing Proteins; D054398:Receptors, CCR4; D019707:Receptors, Chemokine / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection
C106230:CCL17 protein, human; C515354:CCR4 protein, human; C432676:CKLF protein, human; D054414:Chemokine CCL17; D018925:Chemokines; D019742:Chemokines, CC; D008024:Ligands; D062792:MARVEL Domain-Containing Proteins; D054398:Receptors, CCR4; D019707:Receptors, Chemokine; D011994:Recombinant Proteins; D002118:Calcium