title: Higher plant RecA-like protein is homologous to RadA.
abstract: A novel RecA-like protein, differing from Dmc1 and Rad51, was characterized in Oryza sativa L. cv. Nipponbare. Because the protein is homologous to bacterial RadA, the gene was designated OsRadA. The open reading frame was predicted to encode a 66kDa protein of 619 amino acid residues and was found in plants but not animals or yeast. OsRadA showed D-loop and single-stranded DNA-dependent ATPase activities. Gene expression was found to be high in meristematic tissues, and was localized in the nucleus. An RNAi mutant of Arabidopsis thaliana RadA (AtRadA) was sensitive to mutagenic agents such as UV and MMC, suggesting that RadA functions in DNA repair.
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan.
authors.forename: Toyotaka
authors.initials: T
authors.lastname: Ishibashi
authors.forename: Minako
authors.initials: M
authors.lastname: Isogai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kiyohara
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hosaka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Chiku
authors.forename: Asami
authors.initials: A
authors.lastname: Koga
authors.forename: Taichi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Uchiyama
authors.forename: Yoko
authors.initials: Y
authors.lastname: Mori
authors.forename: Junji
authors.initials: J
authors.lastname: Hashimoto
authors.forename: Juan
authors.initials: J
authors.lastname: Ausió
authors.forename: Seisuke
authors.initials: S
authors.lastname: Kimura
authors.forename: Kengo
authors.initials: K
authors.lastname: Sakaguchi
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D019843:Archaeal Proteins / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D049109:Cell Proliferation; D003001:Cloning, Molecular; D004260:DNA Repair / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D016685:Mitomycin / Q000009:adverse effects; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000166:cytology / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D011938:Rec A Recombinases / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D029681:Arabidopsis Proteins; D019843:Archaeal Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D010940:Plant Proteins; C117252:RadA protein, archaeal; C100901:RadA protein, bacteria; D016685:Mitomycin; D011938:Rec A Recombinases