[Conjugates of pancreatic ribonuclease and ligand-free human serum albumin].
Conjugates of pancreatic RNase and ligand-free human serum albumin (LFHSA) have been obtained. The number of hydrophobic binding sites both for initial HSA and LFHSA has been determined by the polarised luminescence method. Interaction between RNase and HSA involves additional electrovalent linkage. Unlike initial enzyme, conjugates exhibit activity toward double-strand RNA. After intravenous injection, transferase activity of unmodified enzyme remains in the blood during 20 min., whereas 30-40% of this activity is detected at the fourth day after administration of RNase conjugates. A single dose administration of LFHSA-RNAse conjugates exhibited high antiviral activity in mice, infected with influenza A and influenza B viruses.
Zelepuga
E A
EA
Ponomareva
R B
RB
Kolikov
V M
VM
Pautov
V D
VD
Sheveleva
T V
TV
Medvedev
M L
ML
Tret'iak
T M
TM
Trepelovskaia
O N
ON
D000818:Animals; D001665:Binding Sites; D006801:Humans; D008024:Ligands; D008657:Metabolic Clearance Rate; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D014018:Tissue Distribution
D008024:Ligands; D012709:Serum Albumin; D012259:Ribonuclease, Pancreatic