title: Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines.
abstract: The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT-PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic polymer peptides from this protein produced antibodies specifically recognizing a 25-kDa band in mycobacterial sonicate. U937 and A549 cells were used in binding assays involving 20-amino-acid-long synthetic peptides covering the whole Rv2536 protein sequence. Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY180) presented high specific binding to both types of cells; the binding was saturable and presented nanomolar affinity constants. Cross-linking assays revealed that this peptide specifically binds to 50 kDa U937 cell membrane and 45 kDa A549 cell membrane proteins.
authors.affiliation: Fundación Instituto de Inmunologia de Colombia and the Universidad Nacional de Colombia, Avda. Calle 26 No. 5000, Bogotá, Colombia 020304. javgar22@hotmail.com
authors.forename: Javier
authors.initials: J
authors.lastname: García
authors.forename: Alvaro
authors.initials: A
authors.lastname: Puentes
authors.forename: Luis
authors.initials: L
authors.lastname: Rodríguez
authors.forename: Marisol
authors.initials: M
authors.lastname: Ocampo
authors.forename: Hernando
authors.initials: H
authors.lastname: Curtidor
authors.forename: Ricardo
authors.initials: R
authors.lastname: Vera
authors.forename: Ramses
authors.initials: R
authors.lastname: Lopez
authors.forename: John
authors.initials: J
authors.lastname: Valbuena
authors.forename: Jimena
authors.initials: J
authors.lastname: Cortes
authors.forename: Magnolia
authors.initials: M
authors.lastname: Vanegas
authors.forename: Carlos
authors.initials: C
authors.lastname: Barrero
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Patarroyo
authors.forename: Mauricio
authors.initials: M
authors.lastname: Urquiza
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Patarroyo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line / Q000378:metabolism*; D002942:Circular Dichroism; D003432:Cross-Linking Reagents; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007106:Immune Sera; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000235:genetics*; D011817:Rabbits; D020298:U937 Cells
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D007106:Immune Sera; D008565:Membrane Proteins