Phytotoxic effects and chemical analysis of leaf extracts from three Phytolaccaceae species in South Korea.
We analyzed phenolic compounds and other elements in leaf extracts and compared morphology of three species of the Phytolaccaceae family found in South Korea. To test allelochemical effects of the three Phytolacca species, we also examined seed germination and dry weight of seedlings of Lactuca indica and Sonchus oleraceus treated with leaf extracts. The concentrations of total phenolic compounds were exotic Phytolacca esculenta (3.9 mg/l), native Phytolacca insularis (4.4 mg/l), and exotic Phytolacca americana (10.2 mg/l). There was no significant difference in concentrations between P. esculenta and P. insularis, but the concentration of total phenolics in P. americana was two times higher than either P. esculenta or P. insularis. Analysis of aqueous extracts by HPLC showed seven phenolic compounds (gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, m-hydroxybenzoic acid, p-coumaric acid, and cinnamic acid). Total phenolics in P. americana were eight to 16 times higher than either P. esculenta or P. insularis, respectively. P. americana inhibited seed germination and dry weight of the two assay species. The phytotoxic effects of the two Phytolacca species were different, despite the fact that P. esculenta and P. insularis had similar levels of total phenolic compounds. We also found that P. americana had invaded Ullung Island, which suggested that P. americana had excellent adaptability to the environment. The three species of Phytolaccaceae in South Korea can be distinguished by their different allelopathic potentials and morphologies.
Kim
Yong Ok
YO
Natural Resource Sciences Department, Washington State University, Puyallup WA 98371-4998, USA.
Johnson
Jon D
JD
Lee
Eun Ju
EJ
D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D018525:Germination; D007723:Korea; D018545:Lactuca / Q000254:growth & development; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D029602:Phytolaccaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D012639:Seeds; D031184:Sonchus / Q000254:growth & development
D010636:Phenols; D010936:Plant Extracts