title: Shear stress alters the expression of myelin-associated glycoprotein (MAG) and myelin basic protein (MBP) in Schwann cells.
abstract: Schwann cells within a peripheral nerve respond robustly after an axonal injury. Recent results have revealed that Schwann cells undergo concurrent proliferation and apoptosis after a chronic nerve injury that is independent of axonal pathology. Although the exact nature of the stimulus that produces this Schwann cell response remains unknown, we postulated that this response may be triggered directly by mechanical stimuli. Thus, we sought to determine how pure Schwann cells responded to a sustained shear stress in the form of laminar fluid flow by evaluating for proliferation, expression of S-100, myelin-associated glycoprotein (MAG), and myelin basic protein (MBP). Immunohistochemistry demonstrated that the Schwann cells were positive for S-100, MAG, and MBP in greater than 99% of the experimental cells. Stimulated cells also revealed an increased rate of proliferation by as much as 100% (p<.001). The mRNA expression of MAG and MBP was down-regulated by 21% (p<.035) and 18% (p<.015), respectively, in experimental cells from RT-PCR assays. Furthermore, Western blot showed a down-regulation in MAG and MBP protein expression by 29% (p<.035) and 35% (p<.02), respectively. This study provides novel information regarding Schwann cell direct response to this physical stimulus that is not secondary to an axonal injury.
authors.affiliation: Peripheral Nerve Research Lab, Department of Orthopedic Surgery, University of California, Irvine, CA 92697, USA. ranjang@uci.edu
authors.forename: Ranjan
authors.initials: R
authors.lastname: Gupta
authors.forename: Linh
authors.initials: L
authors.lastname: Truong
authors.forename: David
authors.initials: D
authors.lastname: Bear
authors.forename: Dara
authors.initials: D
authors.lastname: Chafik
authors.forename: Edward
authors.initials: E
authors.lastname: Modafferi
authors.forename: Clark T
authors.initials: CT
authors.lastname: Hung
mesh_terms: D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D004676:Myelin Basic Protein / Q000032:analysis / Q000235:genetics*; D018992:Myelin-Associated Glycoprotein / Q000032:analysis / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D013314:Stress, Mechanical
chemical_list: D004676:Myelin Basic Protein; D018992:Myelin-Associated Glycoprotein