[Expression of the heat shock protein-70 in the myocardial cells of cyanosis congenital heart diseases].
OBJECTIVE
To evaluate the expression and myocardial protection of heat shock protein-70 (HSP70) in the myocardial cells of cyanosis congenital heart diseases.


METHODS
The study enrolled 24 patients with congenital heart disease: 12 cases of non-cyanosis (group A), and the other 12 cases of cyanosis (group B). During the operation the samples of myocardium in the right atrium were attained at 0 min ischemia, 20 min ischemia, and 20 min reperfusion. They were used to evaluate the content of HSP70 by immunohistochemistry and activation of SOD and content of MDA by chemochromatometry.


RESULTS
The content of HSP70 in myocardial cells during the operation was not significantly different in both groups (P > 0. 05). The content of HSP70 significantly decreased in group B compared with that of group A (P <0.05). The activity of SOD significantly decreased and the content of MDA significantly increased in group B during the operation (P < 0.05).


CONCLUSIONS
Decreased expression of HSP70 is associated with the decreased tolerance to ischemia-reperfusion injury in myocardial cells of cyanosis congenital heart disease.
Wen
Shi-Bing
SB
Department of Cardiothoracic Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Hu
Jian-Guo
JG
Ma
Zhong-Xia
ZX
Zhou
Xin-Min
XM
Yang
Yi-Feng
YF
Yu
Feng-Lei
FL
Hu
Min-Song
MS
D004310:Double Outlet Right Ventricle / Q000378:metabolism*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D013771:Tetralogy of Fallot / Q000378:metabolism*
D018840:HSP70 Heat-Shock Proteins; D013482:Superoxide Dismutase