Postprandial, but not postabsorptive low-density lipoproteins increase the expression of intercellular adhesion molecule-1 in human aortic endothelial cells.
The magnitude of postprandial lipemia has been identified as independent risk factor for the development of coronary artery disease. To test the effect of postprandial versus postabsorptive low-density lipoproteins (LDL) on the expression of adhesion molecules, LDL were isolated from healthy subjects before and 4h after ingestion of a standardized fatty test meal. We used flow cytometry and Northern blotting to quantify cell adhesion molecules in human aortic endothelial cells (HAEC). The adherence of leukocytes to HAEC was analyzed using a monocyte adhesion assay. Incubation of HAEC with postprandial, but not postabsorptive LDL induced a two-fold increase in the surface expression of intercellular adhesion molecule-1 (ICAM-1), but not of E-selectin or vascular cell adhesion molecule-1. In addition, increased amounts of ICAM-1 transcripts were found in HAEC treated with postprandial LDL. The adhesion of monocytes to HAEC was enhanced after pretreatment with postprandial, but not with postabsorptive LDL. We conclude that postprandial, but not postabsorptive LDL increase the surface expression of ICAM-1 in HAEC apparently by de novo protein synthesis leading to increased adhesion of monocytes. The upregulation of ICAM-1 by postprandial LDL may explain part of the proatherogenic effect of high postprandial lipemia.
Marschang
Peter
P
Clinical Division of General Internal Medicine, Clinical Department of Internal Medicine, Innsbruck Medical University, Anichstr. 35, A-6020 Innsbruck, Austria.
Götsch
Claudia
C
Kirchmair
Rudolf
R
Kaser
Susanne
S
Kähler
Christian M
CM
Patsch
Josef R
JR
D001011:Aorta / Q000166:cytology* / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D015152:Blotting, Northern; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D003327:Coronary Disease / Q000097:blood / Q000209:etiology; D050171:Dyslipidemias / Q000097:blood / Q000150:complications; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D019518:Postprandial Period / Q000502:physiology*; D012313:RNA / Q000235:genetics*; D012307:Risk Factors
D015415:Biomarkers; D008077:Lipoproteins, LDL; D018799:Intercellular Adhesion Molecule-1; D012313:RNA