Developing a biosensor for estrogens in water samples: study of the real-time response of live cells of the estrogen-sensitive yeast strain RMY/ER-ERE using fluorescence microscopy.
Using a fluorescein di-beta-D-galactopyranoside (FDG) substrate we show that in live cells of an estrogen-sensitive yeast strain RMY/ER-ERE with human estrogen receptor (ERalpha) gene and the lacZ gene which encodes beta-galactosidase, the uptake of 17beta-estradiol (E2) and the subsequent production of beta-galactosidase enzyme occur quite rapidly, with maximal enzyme-catalyzed product formation evident after about 30 min of exposure to E2. This finding which agrees with the well-known rates of enzyme-catalyzed reactions could have implications for shortening the duration of environmental sample screening and monitoring regimes using yeast-based estrogen assays, and the development of biosensors for environmental estrogens to complement quantification methods.
Wozei
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Lawrence Berkeley National Laboratory, Center for Environmental Biotechnology, MS 70A-3317, Berkeley, CA 94720, USA. EWozei@LBL.gov
Hermanowicz
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SW
Holman
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HY
D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003199:Computer Systems; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004967:Estrogens / Q000032:analysis* / Q000494:pharmacology; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D013045:Species Specificity; D014873:Water Pollutants / Q000032:analysis* / Q000494:pharmacology
D004967:Estrogens; D014873:Water Pollutants