title: Characterization of phages virulent for Sarothamnus scoparius bradyrhizobia.
abstract: Four virulent phages--PhiDl, PhiTl, PhiCYT21, and PhiOS6, infective on Sarothamnus scoparius rhizobia--were isolated from the soil and characterized for morphology, host range, rate of adsorption to bacterial cells, and genome size. New phages were separated into two morphological families: Siphoviridae with long, noncontractile tails (PhiDl, PhiTl) and Myoviridae with long, contractile tails (PhiCYT21, PhiOS6). They were also classified into two groups by a host specificity. One of them included viruses (PhiDl and PhiTl) that lysed S. scoparius bradyrhizobia and Bradyrhizobium sp. (Lupinus) strain Dl, and the second one comprised phages (PhiCYT21 and PhiOS6) that parasitized only Scotch broom native microsymbionts. Phages specific for S. scoparius rhizobia were differentiated not only by morphology and host range but also by a genome size that was in the range from 47,583 to 60,098 b.p.
authors.affiliation: Department of General Microbiology, M. Curie-Skłodowska University, Lublin, Poland. wanda@biotop.umcs.lublin.pl
authors.forename: Wanda
authors.initials: W
authors.lastname: Małek
authors.forename: Ewa
authors.initials: E
authors.lastname: Sajnaga
authors.forename: Sylwia
authors.initials: S
authors.lastname: Wdowiak-Wróbel
authors.forename: Bozena
authors.initials: B
authors.lastname: Studzińska
authors.forename: Izabela Swie
authors.initials: IS
authors.lastname: Icka
authors.forename: Izabela
authors.initials: I
authors.lastname: Nosalewicz
authors.forename: Marta
authors.initials: M
authors.lastname: Słomka
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Tatara
authors.forename: Antoni
authors.initials: A
authors.lastname: Gawron
mesh_terms: D041841:Bradyrhizobiaceae / Q000502:physiology / Q000821:virology*; D029865:Cytisus / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D008854:Microscopy, Electron; D017900:Myoviridae / Q000302:isolation & purification / Q000472:pathogenicity* / Q000648:ultrastructure; D017906:Siphoviridae / Q000302:isolation & purification / Q000472:pathogenicity* / Q000648:ultrastructure; D012988:Soil Microbiology; D014774:Virulence
chemical_list: D004279:DNA, Viral