title: Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies.
abstract: Fourteen hepatitis C virus (HCV) chronically infected patients were submitted to routine liver biopsy for histological evaluation. Liver samples were assayed to HCV-RNA by in situ hybridization, using digoxigenin labeled probe. HCV genotypes were found to be predominantly type 1 (71.4%), followed by genotype 3 (21.4%), and genotype 2 (7.2%). Alanine-aminotransferase levels were raised in 10 patients. The histopathological scores were minimal (21.4%), mild (57.2%), and moderate (21.4%). Viral RNA was detected in liver cells from nine patients (64.3%). ISH method provides localization and poor confirmation of HCV RNA in the liver tissue of HCV chronic patients.
authors.affiliation: Departamento de Virologia, Instituto Oswaldo Cruz, Fiocruz, 21045-900 Rio de Janeiro, RJ, Brazil. hustenc@hotmail.com
authors.forename: H S
authors.initials: HS
authors.lastname: Carvalho
authors.forename: M L
authors.initials: ML
authors.lastname: Baptista
authors.forename: M A
authors.initials: MA
authors.lastname: Pinto
authors.forename: M
authors.initials: M
authors.lastname: Silva
authors.forename: C M
authors.initials: CM
authors.lastname: Takiya
authors.forename: V
authors.initials: V
authors.lastname: Chagas
authors.forename: V
authors.initials: V
authors.lastname: Pannain
authors.forename: H S M
authors.initials: HS
authors.lastname: Coelho
authors.forename: C F T
authors.initials: CF
authors.lastname: Yoshida
mesh_terms: D000328:Adult; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood; D001706:Biopsy; D004076:Digoxigenin; D005260:Female; D005557:Formaldehyde; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D019698:Hepatitis C, Chronic / Q000473:pathology / Q000821:virology*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008099:Liver / Q000473:pathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D012150:Polymorphism, Restriction Fragment Length; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index
chemical_list: D012367:RNA, Viral; D005557:Formaldehyde; D000410:Alanine Transaminase; D004076:Digoxigenin