Magnetically responsive carboxylated magnetite-polydipyrrole/polydicarbazole nanocomposites of core-shell morphology. Preparation, characterization, and use in DNA hybridization.
Novel bis-heterocyclic mono- and dicarboxylated dipyrrole and dicarbazole monomers have been synthesized in a modular manner. Their oxidative polymerization around magnetite nanosized particles has been investigated and optimized toward new magnetic magnetite-polydipyrrole/polydicarbazole nanocomposites (NCs) of a core-shell morphology. These NCs were thoroughly characterized by FT-IR, TGA (Thermal Gravimetric Analysis), low- and high-resolution TEM/HR-TEM microscopies, and Mössbauer spectroscopy along with magnetization studies. Exploiting the versatile COOH chemistry (activation by water-soluble diimides) introduced by the polymeric shell, DNA hybridization experiments have been conducted onto NC surfaces using an efficient blue-colored HRP-based enzymatic screening biological system. Highly parallel NC-supported DNA hybridization experimentations revealed that these NCs presented an interesting potential for DNA-based diagnostic applications.
Lellouche
Jean-Paul
JP
Department of Chemistry, Bar-Ilan University, Ramat-Gan 52900, Israel. lellouj@mail.biu.ac.il
Senthil
Govindaraji
G
Joseph
Augustine
A
Buzhansky
Ludmila
L
Bruce
Ian
I
Bauminger
Erika R
ER
Schlesinger
Jacob
J
D001672:Biocompatible Materials / Q000737:chemistry; D002227:Carbazoles / Q000737:chemistry*; D002264:Carboxylic Acids / Q000737:chemistry; D015342:DNA Probes; D006571:Heterocyclic Compounds / Q000737:chemistry; D008280:Magnetics; D008956:Models, Chemical; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D001672:Biocompatible Materials; D002227:Carbazoles; D002264:Carboxylic Acids; D015342:DNA Probes; D006571:Heterocyclic Compounds; D011108:Polymers; D011758:Pyrroles