title: [Dynamic pattern of gene expression in rat hearts responding to transient ischemia/reperfusion detected by cDNA microarray and cluster analysis].
abstract: OBJECTIVE
  To analyze the pattern of gene expression programs during rat myocardial ischemia/reperfusion at multiple time points.
  
  
  METHODS
  Rat model of myocardial ischemia/reperfusion was established by repeating the occlusion and relaxation of left coronary artery. cDNA microarray was used to analyze the pattern of gene expression programs in rat myocardium at 1, 3, 6, 12, 24 hours after reperfusion. SOM cluster analysis was used to identify different cluster of genes in which each cluster had similar expression pattern.
  
  
  RESULTS
  Altogether 75, 779, 205, 155, and 166 genes were differentially expressed at 1, 3, 6, 12, 24 hours after the reperfusion respectively. Clusters analysis identified 12 clusters of genes in which each cluster had similar expression pattern.
  
  
  CONCLUSIONS
  Analysis of gene expression pattern revealed sequential induction of subsets of genes that characterize each response.
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China. xianzhongxiao@hotmail.com
authors.forename: Can
authors.initials: C
authors.lastname: Yuan
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Qing-Lan
authors.initials: QL
authors.lastname: Lu
authors.forename: Qiu-Peng
authors.initials: QP
authors.lastname: Wang
authors.forename: Xian-Zhong
authors.initials: XZ
authors.lastname: Xiao
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000235:genetics*; D009206:Myocardium / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes; D051381:Rats; D013997:Time Factors
chemical_list: D015345:Oligonucleotide Probes