Establishment of a transplantable human myeloid BALB/c nude mouse model.
To establish a mouse model bearing transplantable human chronic myeloid leukemia for hematopoietic stem cell transplantation to treat leukemia, 4 - 5-week-old female BALB/c nude mice were given cyclophosphamide 2 mg/mouse at day -2, -1, and then the human chronic myeloid leukemia K562 cells were engrafted into the mice at day 0 by injection via tail vein or peritoneal cavity. PB and BM cells were collected, the CD45, CD13, and CD33 antigens were delected by using FCM, the bcr/abl fusion gene mRNA was examined by RT-PCR. The results showed that transplantable leukemic mice could be yielded from 4 - 5-week-old nude mice either by injection through tail vein or peritoneal cavity when the total number of inoculated tumor cells was more than 2 x 10(5) per mouse, whether being pretreated with 2 mg CTX/mouse or not. The transplanted mice could survive 30 - 60 day with leukemia. In conclusion, the mouse model bearing leukemia can be established by inoculation 2 x 10(5) K562 cells into immunodeficient BALB/c nude mice.
Wei
Ya-Ming
YM
Department of Blood Transfusion, Nanfang Hospital, The First Military Medical University, Guangzhou 510515, China. weiyaming@163.com
Lin
Ji-Hong
JH
Xia
Rong
R
Lan
Jun-Cai
JC
D000818:Animals; D015703:Antigens, CD / Q000097:blood; D015214:Antigens, Differentiation, Myelomonocytic / Q000097:blood; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D018826:CD13 Antigens / Q000097:blood; D003520:Cyclophosphamide / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D007942:Leukemia, Experimental / Q000097:blood / Q000235:genetics / Q000473:pathology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D063268:Sialic Acid Binding Ig-like Lectin 3; D014183:Transplantation, Heterologous
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018906:Antineoplastic Agents, Alkylating; C571163:CD33 protein, human; C571165:Cd33 protein, mouse; D012333:RNA, Messenger; D063268:Sialic Acid Binding Ig-like Lectin 3; D003520:Cyclophosphamide; D016044:Fusion Proteins, bcr-abl; D018826:CD13 Antigens