Cutting edge: proangiogenic properties of alternatively activated dendritic cells.
Angiogenesis plays an important role in tissue remodeling and repair during the late phase of inflammation. In the present study, we show that human dendritic cells (DC) that matured in the presence of anti-inflammatory molecules such as calcitriol, PGE2, or IL-10 (alternatively activated DC) selectively secrete the potent angiogenic cytokine vascular endothelial growth factor (VEGF) isoforms VEGF165 and VEGF121. No VEGF production was observed in immature or classically activated DC. Also, the capacity to produce VEGF was restricted to the myeloid DC subset. When implanted in the chick embryo chorioallantoic membrane, alternatively activated DC elicit a marked angiogenic response, which is inhibited by neutralizing anti-VEGF Abs and by the VEGFR-2 inhibitor SU5416. Therefore, alternatively activated DC may contribute to the resolution of the inflammatory reaction by promoting VEGF-induced angiogenesis.
Riboldi
Elena
E
Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia, Brescia, Italy.
Musso
Tiziana
T
Moroni
Emanuela
E
Urbinati
Chiara
C
Bernasconi
Sergio
S
Rusnati
Marco
M
Adorini
Luciano
L
Presta
Marco
M
Sozzani
Silvano
S
D002117:Calcitriol / Q000494:pharmacology; D003713:Dendritic Cells / Q000502:physiology*; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology; D018919:Neovascularization, Physiologic; D012333:RNA, Messenger / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000502:physiology
D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D016753:Interleukin-10; D040301:Vascular Endothelial Growth Factor Receptor-2; D002117:Calcitriol