Targeting of the virulence factor acetohydroxyacid synthase by sulfonylureas results in inhibition of intramacrophagic multiplication of Brucella suis.
The acetohydroxyacid synthase (AHAS) of Brucella suis can be effectively targeted by the sulfonylureas chlorimuron ethyl and metsulfuron methyl. Growth in minimal medium was inhibited, and multiplication in human macrophages was totally abolished with 100 microM of sulfonylureas. Metsulfuron methyl-resistant mutants showed reduced viability in macrophages and reduced AHAS activity.
Boigegrain
Rose-Anne
RA
INSERM U-431, Université Montpellier II, CC 100, Place E. Bataillon, F-34095 Montpellier, France.
Liautard
Jean-Pierre
JP
Köhler
Stephan
S
D000095:Acetolactate Synthase / Q000037:antagonists & inhibitors*; D001190:Arylsulfonates / Q000494:pharmacology; D041021:Brucella suis / Q000187:drug effects* / Q000254:growth & development*; D003470:Culture Media; D016503:Drug Delivery Systems; D008264:Macrophages / Q000382:microbiology*; D013453:Sulfonylurea Compounds / Q000494:pharmacology*; D037521:Virulence Factors
D001190:Arylsulfonates; D003470:Culture Media; D013453:Sulfonylurea Compounds; D037521:Virulence Factors; C050296:metsulfuron methyl; D000095:Acetolactate Synthase