[The acquisition of two silkworm CAPs markers and their use in genetic diversity and phylogenetic relationship].
Cleaved amplified polymorphic sequence (CAPs) markers are based on PCR amplification of known genes, cDNA sequences or RAPD sequences. The PCR products are digested by restriction enzymes, generating the simple type of data as heterozygotes and homozygotes. Here we designed primers based on silkworm attacin and alpha-amylase genes, then digested the PCR products in silkworm strains P50, C108 and their progeny F1 using 4 different restriction enzymes respectively. Furthermore, the genetic diversity and phylogenetic relationship of 12 silkworm strains were investigated using the obtained two CAPs markers.
Huang
Jian-Hua
JH
Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. jhhuang@iris.sipp.ac.cn
Miao
Xue-Xia
XX
Li
Mu-Wang
MW
Zhang
Yong
Y
Zhao
Wei-Guo
WG
Huang
Yong-Ping
YP
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000145:classification / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation; D019476:Insect Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid; D000516:alpha-Amylases / Q000235:genetics
D017931:DNA Primers; D005819:Genetic Markers; D019476:Insect Proteins; C039711:attacin antibacterial protein, insect; D000516:alpha-Amylases