Genome-wide map of nucleosome acetylation and methylation in yeast.
Eukaryotic genomes are packaged into nucleosomes whose position and chemical modification state can profoundly influence regulation of gene expression. We profiled nucleosome modifications across the yeast genome using chromatin immunoprecipitation coupled with DNA microarrays to produce high-resolution genome-wide maps of histone acetylation and methylation. These maps take into account changes in nucleosome occupancy at actively transcribed genes and, in doing so, revise previous assessments of the modifications associated with gene expression. Both acetylation and methylation of histones are associated with transcriptional activity, but the former occurs predominantly at the beginning of genes, whereas the latter can occur throughout transcribed regions. Most notably, specific methylation events are associated with the beginning, middle, and end of actively transcribed genes. These maps provide the foundation for further understanding the roles of chromatin in gene expression and genome maintenance.
Pokholok
Dmitry K
DK
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA.
Harbison
Christopher T
CT
Levine
Stuart
S
Cole
Megan
M
Hannett
Nancy M
NM
Lee
Tong Ihn
TI
Bell
George W
GW
Walker
Kimberly
K
Rolfe
P Alex
PA
Herbolsheimer
Elizabeth
E
Zeitlinger
Julia
J
Lewitter
Fran
F
Gifford
David K
DK
Young
Richard A
RA
D000107:Acetylation; D002874:Chromosome Mapping / Q000379:methods; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics; D016681:Genome, Fungal; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D008745:Methylation; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics
D006657:Histones; D009707:Nucleosomes