Electrochemical detection of single a-g mismatch using biosensing surface based on gold nanoparticles.
The study of small drug molecules interacting with nucleic acids is an area of intense research that has particular relevance in our understanding of relative mechanism in chemotherapeutic applications and the association between genetics (including sequence variation) and drug response. In this contribution, we demonstrate how the sequence-specific binding of an anticancer drug Dacarbazine (DTIC) to single base (A-G) mismatch could be sensitively detected by combining electrochemical detection with biosensing surface based on gold nanoparticles.
Zhang
Ren-Yun
RY
Chien-Shiung Wu Laboratory, National Lab of Molecular and Biomolecular Electronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China. xuewang@seu.edu.cn
Wang
Xue-Mei
XM
Gong
Sheng-Jin
SJ
He
Nong-Yue
NY
D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D003606:Dacarbazine / Q000378:metabolism*; D004563:Electrochemistry; D006046:Gold; D007202:Indicators and Reagents; D049329:Nanostructures; D009841:Oligonucleotides / Q000378:metabolism
D007202:Indicators and Reagents; D009841:Oligonucleotides; D006046:Gold; D003606:Dacarbazine