[Nitric oxide synthase expression and multiple drug resistance in patients with acute leukemia].
OBJECTIVE
To determine the relationship between nitric oxide synthase (NOS) expression and Pgp/mdr-1.


METHODS
The expressions of iNOS, eNOS, and Pgp were detected by immunohistochemistry, and the expressions of iNOS gene and mdr-1 gene were detected by RT-PCR.


RESULTS
NOS expression increased in patients with acute leukemia compared with that of the controls. There was some correlation between iNOS and Pgp/mdr-1 gene expression.


CONCLUSIONS
NO may influcence multiple drug resistance in patients with acute leukemia.
Zhu
Yan
Y
Department of Hematology, Xiangya Hospital, Central South University, Changsha 41008, China.
Xie
Zhao-Xia
ZX
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis* / Q000235:genetics; D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005260:Female; D019450:Genes, MDR / Q000235:genetics; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis / Q000235:genetics
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III