title: Effects of removal of necrotic blastomeres from mouse cryopreserved embryos on blastocyst formation and hatching.
abstract: To evaluate whether the developmental potential of embryos that were partially damaged after freezing and thawing can be improved by removal of necrotic blastomeres. Eight-cell mouse embryos were cryopreserved using 1,2-propanediol and sucrose as cryoprotectant with slow cooling procedure. After thawing, blastocyst formation and hatching of fully intact embryos were compared between no treatment and with laser-assisted hatching. For partially intact embryos, the effects of removal of necrotic blastomeres with micromanipulation were evaluated. Laser-assisted hatching of mouse cryopreserved fully intact embryos significantly increased blastocyst hatching (63.4% versus 48.3%, P<0.05), but had little effect on blastocyst formation (72.0% versus 70.1%, P>0.05). The removal of necrotic blastomeres from partially damaged mouse cryopreserved embryos with micromanipulation significantly increased blastocyst formation (52.9% versus 32.0%, P<0.05) and blastocyst hatching (41.2% versus 22.0%, P>0.05) compared with the control group. The developmental potential of partially damaged cryopreserved embryos can be improved by removal of necrotic blastomeres with micromanipulation.
authors.affiliation: Department of Physiology, Sichuan University, Chengdu 610041, China. liuweixind@163.com
authors.forename: Wei-Xin
authors.initials: WX
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Meng-Jun
authors.initials: MJ
authors.lastname: Luo
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Yue
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: He
authors.forename: Jin-Hu
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D001757:Blastomeres / Q000473:pathology / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary*; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D007834:Lasers; D051379:Mice; D009336:Necrosis