title: Syngeneic mouse mammary carcinoma cell lines: two closely related cell lines with divergent metastatic behavior.
abstract: Two cell lines, Met-1(fvb2) and DB-7(fvb2), with different metastatic potential, were derived from mammary carcinomas in FVB/N-Tg(MMTV-PyVmT) and FVB/N-Tg(MMTV-PyVmT ( Y315F/Y322F )) mice, transplanted into syngeneic FVB/N hosts and characterized. The lines maintain a stable morphological and biological phenotype after multiple rounds of in vitro culture and in vivo transplantation. The Met-1(fvb2) line derived from a FVB/N-Tg(MMTV-PyVmT) tumor exhibits invasive growth and 100% metastases when transplanted into the females FVB/N mammary fat pad. The DB-7(fvb2) line derived from the FVB/N-Tg(MMTV-PyVmT ( Y315F/Y322F )) with a "double base" modification at Y315F/Y322F exhibits more rapid growth when transplanted into the mammary fat pad, but a lower rate of metastasis (17%). The Met1(fvb2) cells show high activation of AKT, while DB-7(fvb2) cells show very low levels of AKT activation. The DNA content and gene expression levels of both cell lines are stable over multiple generations. Therefore, these two cell lines provide a stable, reproducible resource for the study of metastasis modulators, AKT molecular pathway interactions, and gene target and marker discovery.
authors.affiliation: Department of Medical Pathology and Laboratory Medicine, UC Davis School of Medicine, Sacramento, CA 95817, USA. adborowsky@ucdavis.edu
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Borowsky
authors.forename: Ruria
authors.initials: R
authors.lastname: Namba
authors.forename: Lawrence J T
authors.initials: LJ
authors.lastname: Young
authors.forename: Kent W
authors.initials: KW
authors.lastname: Hunter
authors.forename: J Graeme
authors.initials: JG
authors.lastname: Hodgson
authors.forename: Clifford G
authors.initials: CG
authors.lastname: Tepper
authors.forename: Erik T
authors.initials: ET
authors.lastname: McGoldrick
authors.forename: William J
authors.initials: WJ
authors.lastname: Muller
authors.forename: Robert D
authors.initials: RD
authors.lastname: Cardiff
authors.forename: Jeffrey P
authors.initials: JP
authors.lastname: Gregg
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D009362:Neoplasm Metastasis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt; D011960:Receptors, Estrogen / Q000032:analysis / Q000378:metabolism
chemical_list: D011518:Proto-Oncogene Proteins; D011960:Receptors, Estrogen; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt