title: Ionic liquid-based aqueous two-phase system, a sample pretreatment procedure prior to high-performance liquid chromatography of opium alkaloids.
abstract: An ionic liquid, 1-butyl-3-methylimidazolium chloride ([C4 mim]Cl)/salt aqueous two-phase systems (ATPS) was presented as a simple, rapid and effective sample pretreatment technique coupled with high-performance liquid chromatography (HPLC) for analysis of the major opium alkaloids in Pericarpium papaveris. To find optimal conditions, the partition behaviors of codeine and papaverine in ionic liquid/salt aqueous two-phase systems were investigated. Various factors were considered systematically, and the results indicated that both the pH value and the salting-out ability of salt had great influence on phase separation. The recoveries of codeine and papaverine were 90.0-100.2% and 99.3-102.0%, respectively, from aqueous samples of P. papaveris by the proposed method.
authors.affiliation: The Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors.forename: Shehong
authors.initials: S
authors.lastname: Li
authors.forename: Chiyang
authors.initials: C
authors.lastname: He
authors.forename: Huwei
authors.initials: H
authors.lastname: Liu
authors.forename: Kean
authors.initials: K
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003061:Codeine / Q000302:isolation & purification*; D007093:Imidazoles; D010207:Papaver / Q000737:chemistry; D010208:Papaverine / Q000302:isolation & purification*; D010710:Phosphates; D017680:Potassium Compounds; D013696:Temperature
chemical_list: D007093:Imidazoles; D010710:Phosphates; D017680:Potassium Compounds; C502841:1-butyl-3-methylimidazolium chloride; C013216:potassium phosphate; D010208:Papaverine; D003061:Codeine