title: Coagulation factor XIII-A. A flow cytometric intracellular marker in the classification of acute myeloid leukemias.
abstract: The association of coagulation factors with leukocytes have been demonstrated in several previous studies. This study was designed to study the sensitivity and specificity of factor XIII subunit A (FXIII-A) labelling in cultured myeloblastic and monoblastic cell lines and to investigate the intracytoplasmic expression of FXIII-A in de novo acute myeloid leukemia (AML) samples. Myeloblastic and a monoblastic cell lines were cultured and investigated for lineage specific maturation markers and FXIII-A expression. Furthermore, FXIII-A expression was investigated in 12 normal samples (7 bone marrow and 5 peripheral blood), 86 de novo AML samples and 6 chronic myelomonocytic leukemia (CMML) samples. In the monoblastic MonoMac6 cell line the appearance of FXIII-A preceded that of CD14 while it remained negative in the myeloblastic PLB-985 cell line throughout its maturation period. Among the AML samples the average frequency of FXIII-A positive cells in myeloblastic leukemia samples was below 10%, while in M4 and M5AML samples it was above 50% and was significantly higher than the generally used CD14 marker (p < 0.0001). In the AML M4 and M5 cases, FXIII-A proved sensitive for the identification of monoblasts. FXIII-A can be considered as a reliable intracytoplasmic marker for the monocytic and megakaryocytic series and its presence is highly predictive for mono- and megakaryocytic AML and for CMML.
authors.affiliation: Department of Clinical Biochemistry and Molecular Pathology, Medical and Health Science Centre, University of Debrecen, Hungary. kappelmayer@dote.hu
authors.forename: János
authors.initials: J
authors.lastname: Kappelmayer
authors.forename: Agnes
authors.initials: A
authors.lastname: Simon
authors.forename: Eva
authors.initials: E
authors.lastname: Katona
authors.forename: Attila
authors.initials: A
authors.lastname: Szanto
authors.forename: László
authors.initials: L
authors.lastname: Nagy
authors.forename: Attila
authors.initials: A
authors.lastname: Kiss
authors.forename: Csongor
authors.initials: C
authors.lastname: Kiss
authors.forename: László
authors.initials: L
authors.lastname: Muszbek
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D019070:Cell Lineage; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D026122:Factor XIIIa / Q000378:metabolism* / Q000502:physiology*; D005434:Flow Cytometry / Q000379:methods*; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000145:classification / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D008533:Megakaryocytes / Q000166:cytology; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D022423:Myeloid Cells / Q000166:cytology; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D018950:Lipopolysaccharide Receptors; D026122:Factor XIIIa