Aberrant activation of the TNF-alpha system and production of Fas and scavenger receptors on monocytes in patients with end-stage renal disease.
Ten patients with nondialyzed chronic renal failure (CRF), 14 receiving continuous ambulatory peritoneal dialysis (CAPD), 16 receiving hemodialysis (HD), and 10 normal controls (NC), were evaluated. Levels of Fas antigen (CD95), scavenger receptors (CD36 and CD68), and tumor necrosis factor-receptor 2 (CD120b) on monocytes were measured using flow cytometry. All patients showed lymphocytopenia, and monocyte counts were decreased in those with CRF. Fas levels were higher in patients receiving HD than the others, and were higher in the CRF and CAPD groups than in controls. CD120b levels were similar to those of Fas. Monocyte CD36 levels in the dialysis groups were significantly higher than in the CRF and NC groups. CD68 was also significantly elevated in HD patients. Fas levels were positively correlated with those of CD120b and CD68. The patient groups showed higher levels of apoptotic markers and scavenger receptors, combined with activation of the TNF-alpha system, especially in patients receiving HD.
Wu
Chia-Chao
CC
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
Chen
Jin-Shuen
JS
Lin
Shih-Hua
SH
Chu
Pauling
P
Lin
Yuh-Feng
YF
Lin
Su-Mei
SM
Liao
Tung-Nan
TN
D000368:Aged; D015703:Antigens, CD / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology; D018955:CD36 Antigens / Q000276:immunology; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D051116:Receptors, Scavenger / Q000276:immunology*; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000276:immunology*; D006435:Renal Dialysis; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*; D014511:Uremia / Q000276:immunology; D019014:fas Receptor / Q000276:immunology*; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018955:CD36 Antigens; D051116:Receptors, Scavenger; D047889:Receptors, Tumor Necrosis Factor, Type II; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor; C067980:CD68 antigen, human; D000097382:CD68 Molecule