title: Desalting minimal amounts of DNA for electroporation in E. coli: a comparison of different physical methods.
abstract: Recovery and purification of ligated plasmid DNA is a critical and limiting step in modern molecular cloning techniques prior to electroporation in E. coli. Four different DNA purification and desalting methods were compared in regard to simple handling, reproducibility, efficiency and cost-effectiveness using picogram and nanogram amounts of DNA. Microcolumn purification of DNA was up to two orders of magnitude more efficient compared to gel filtration, ethanol precipitation or drop dialysis.
authors.affiliation: Institut für Laboratoriumsmedizin und Pathobiochemie, Charité Universitätsmedizin Berlin, Campus Virchow-Klinikum Augustenburger Platz 1, D-13353, Berlin, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Schlaak
authors.forename: Peter
authors.initials: P
authors.lastname: Hoffmann
authors.forename: Karin
authors.initials: K
authors.lastname: May
authors.forename: Andreas
authors.initials: A
authors.lastname: Weimann
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial