[A study of inductive effect of hemin on expression of the beta-globin genes in K562 cells].
The recombinant plasmid HG was constructed,in which the reporter gene encoding the enhanced green fluorescent protein (EGFP) was driven by the beta-globin promoter and regulated under the HS2 element. The inductive effect of hemin on the expression of the beta-globin gene and transiently transfected beta-globin genes in K562 cells was analysed by FACS as well as RT-PCR method. The results showed that the level of gamma and beta-globin gene mRNA in K562 cells increased significantly after 24,48 and 72 hours induced with 30 micromol/LHm.And this inductive effect was stronger after 24 and 48 hours. Furthermore,the transient expression of plasmid HG in K562 cells increased significantly with hemin induction. These results indicated that the mechanism of inductive erythroid differentiation with hemin may be correlated with mechanism of gamma-->beta-globin gene.
Jia
Chun-ping
CP
Shanghai Institute of Medical Genetics Shanghai Childrens Hospital, Shanghai 200040, China.
Chen
Mei-jue
MJ
Huang
Shu-zhen
SZ
Zeng
Yi-tao
YT