Estimation of partition coefficients by MEKC part 2: anthocyanins.
The octanol-water partition coefficients of seven anthocyanins (in neutral form, pH = 7.0) were measured by means of MEKC. A new iterative method for the estimation of migration time of electroosmotic flow (EOF) and micelles in MEKC is presented. This calculation is based on Gauss-Newton linearization of the dependence between migration indices and migration times of a set of suitable standards and the application of an advanced statistical evaluation procedure. The values of partition coefficients obtained with the aid of an iterative process are compared with values obtained on use of EOF and micelle markers.
Müller
Lukás
L
Department of Analytical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic. mlluk@post.cz
Bednár
Petr
P
Barták
Petr
P
Lemr
Karel
K
Sevcík
Juraj
J
D000872:Anthocyanins / Q000737:chemistry / Q000302:isolation & purification*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods* / Q000706:statistics & numerical data; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008823:Micelles; D015233:Models, Statistical; D000442:Octanols; D014867:Water
D000872:Anthocyanins; D008823:Micelles; D000442:Octanols; D014867:Water