[The insertion/deletion polymorphism of angiotensin I converting enzyme gene and coronary heart disease--discordant sib pair analysis and transmission/disequilibrium test].
To investigate whether the insertion/deletion polymorphism of the human angiotensin I converting enzyme gene increased the risk of coronary heart disease (CHD) in CHD pedigrees,discordant sib pair analysis (DSP) and transmission/disequilibrium test (TDT) were used. Forty-five CHD pedigrees with at least one CHD patient in the first degree relatives of probands were recruited during Oct. 1998 to Feb. 1999, of which parental genotype known, one or both parental genotype missing was 21.2 and 22 respectively. ACE genotype was measured by PCR technique. Conditional Logistic regression was used to analyze the DSP, and TDT-STDT program 1.1 was used for TDT and STDT. Univariable conditional Logistic regression did not find significant difference of the distribution of three different ACE genotypes in the 106 discordant sib pairs obtained from the 45 pedigrees. After adjusting effects of traditional risk factors of CHD, no significant difference of the distribution was found by multiple Logistic regression model. Neither the TDT for 13 nuclear families or STDT (sib transmission/disequilibrium test) for 24 sibships showed significant difference between the transmitted and untransmitted ACE gene D allele distributions. Our results show that the insertion/deletion polymorphism of ACE gene is not associated or linked with CHD in Chinese population.
Wang
Xiao-Ling
XL
Fuwai Hospital and Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China. wangxl174@263.net
Gu
Dong-Feng
DF
Fan
Zhong-Jie
ZJ
Sun
Feng
F
Yu
Quan-Jun
QJ
Wu
Xi-Gui
XG
Jia
Xuan
X
Zhao
Jian-Gong
JG
Chen
Yan
Y