Sodium dodecyl sulfate agarose gel electropheresis and electroelution of high molecular weight human salivary mucin.
The aim of this study was to test the applicability of sodium dodecyl sulfate (SDS) agarose gel electrophoresis, electroelution and electrophoretic filtration as methods of separation, detection, and purification of high molecular weight human salivary mucin. SDS agarose gel electrophoresis of whole saliva and mucin prepared by density gradient ultracentrifugation revealed bands with molecular weights in excess of 450 kDa and between 1.6x10(6) and 2x10(6) Da. Electroelution of material from gel and subsequent electrophoresis resulted in highly purified high molecular weight material. Electrophoretic filtration, a method of collecting the material remaining from whole saliva in the slot after penetration of low molecular weight constituents into SDS polyacrylamide gel, failed to produce pure high molecular weight material. It is concluded that SDS agarose gel electrophoresis and electroelution are suitable methods for studying high molecular weight salivary mucin glycoproteins.
Gabriel
Matthias
M
Academic Centre for Dentistry Amsterdam (ACTA), Louwesweg 1, 1066 EA, Amsterdam, The Netherlands.
Zentner
Andrej
A
D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005374:Filtration; D006023:Glycoproteins / Q000302:isolation & purification; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight; D009077:Mucins / Q000302:isolation & purification*; D012471:Salivary Proteins and Peptides / Q000302:isolation & purification*
D006023:Glycoproteins; D009077:Mucins; D012471:Salivary Proteins and Peptides