Apoptotic index as predictive marker for radiosensitivity of cervical carcinoma: evaluation of membrane fluidity, biochemical parameters and apoptosis after the first dose of fractionated radiotherapy to patients.
BACKGROUND
This study was aimed to develop possible predictive response of cervical carcinoma in stage IIIA and B patients by evaluating the changes in physical parameter, such as, membrane fluidity, biochemical parameters, such as, intracellular calcium, antioxidant enzymes [superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx)] and apoptotic cell death in cervical cancer cells from patients after treating with the first fractionated dose of 2 Gy in radiation therapy protocol.


METHODS
Biopsies of cervical carcinoma patients were collected before and 24h after first fractionated radiation dose of 2 Gy. Cell suspensions and tissue of cervix cancer biopsies were used to measure various physical and biochemical parameters.


CONCLUSIONS
A negative correlation was found to exist between observed fluidity of membrane/SOD level with the degree of apoptosis in cervical cells. On the other hand, a positive correlation was observed between intracellular calcium level and percent cellular apoptosis. These results suggest that changes in membrane fluidity, SOD and calcium level were involved in the mechanism of radiation induced cervical apoptosis as measured by TUNEL assay. Moreover, apoptotic sensitivity of these cells after the first dose of radiation treatment showed a direct correlation with the radiation treatment outcome in patients after completion of radiotherapy protocol (70 Gy) in the clinic suggesting that apoptotic index may form a basis for prognosis in radiotherapy in stage III cervix cancer patients.
Bhosle
Sushma M
SM
Radiation Oncology Division, Dr. Balabhai Nanavati Hospital, Mumbai 400 056, India.
Huilgol
Nagraj G
NG
Mishra
Kaushala P
KP
D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000528:radiation effects*; D014408:Biomarkers, Tumor / Q000528:radiation effects*; D002118:Calcium / Q000528:radiation effects; D002374:Catalase / Q000528:radiation effects; D019583:Dose Fractionation, Radiation; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005979:Glutathione Peroxidase / Q000528:radiation effects; D006801:Humans; D020287:In Situ Nick-End Labeling; D008560:Membrane Fluidity / Q000528:radiation effects*; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011836:Radiation Tolerance / Q000528:radiation effects*; D013482:Superoxide Dismutase / Q000187:drug effects; D016896:Treatment Outcome; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000532:radiotherapy*
D014408:Biomarkers, Tumor; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002118:Calcium