Inactivation of monocarboxylate transporter MCT3 by DNA methylation in atherosclerosis.
BACKGROUND
Monocarboxylate transporters (MCTs) mediate lactate transport across the plasma membrane of cells. The molecular mechanisms regulating monocarboxylate transport in smooth muscle cells (SMCs) remain poorly characterized. The aim of this study was to investigate the effects of DNA methylation on MCT expression and lactate transport in SMCs in relation to atherosclerosis.


RESULTS
MCT expression was determined by real-time reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry in SMCs isolated from human aortas and coronary arteries. Bisulfite sequencing and confocal microscopic analysis were used to study DNA methylation and lactate transport in SMCs, respectively. Downregulation of MCT3 and impaired lactate transport were detected in proliferating/synthetic SMCs, relative to the contractile phenotype. A passage number- and atherosclerotic lesion-dependent methylation pattern of MCT3 was demonstrated in the CpG island located in exon 2. Treatment of SMCs with the demethylating agent 5-aza-2'-deoxycytidine restored MCT3 expression and normalized lactate transport. Furthermore, small interfering RNA-mediated specific MCT3 knockdown substantially stimulated SMC proliferation.


CONCLUSIONS
These data indicate that DNA methylation may modify monocarboxylate transport by suppressing MCT3 expression, which could be important in regulating SMC function and the development of atherosclerosis.
Zhu
Shoukang
S
Department of Internal Medicine, Duke University Medical Center, Durham, NC 27710, USA.
Goldschmidt-Clermont
Pascal J
PJ
Dong
Chunming
C
D001011:Aorta / Q000378:metabolism; D050197:Atherosclerosis / Q000209:etiology* / Q000378:metabolism; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D001692:Biological Transport; D049109:Cell Proliferation; D002478:Cells, Cultured; D018899:CpG Islands; D019175:DNA Methylation; D000077209:Decitabine; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics*; D027501:Monocarboxylic Acid Transporters; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000494:pharmacology; D027981:Symporters
D026901:Membrane Transport Proteins; D027501:Monocarboxylic Acid Transporters; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C106260:SLC16A3 protein, human; D027981:Symporters; D019344:Lactic Acid; D000077209:Decitabine; D001374:Azacitidine