How we process trephine biopsy specimens: epoxy resin embedded bone marrow biopsies.
Improved cytomorphology of semithin resin sections over paraffin wax embedded sections may be important in diagnostic haematopathology. However, resin embedding can make immunohistochemical antigen detection or DNA isolation for clonal gene rearrangement assays difficult. This review describes the processing of bone marrow biopsies using buffered formaldehyde based fixation and epoxy resin embedding, with or without EDTA decalcification. Traditional semithin resin sections are completely rehydrated after etching in home made sodium methoxide solution. Resin elimination allows high resolution staining of tissue components with common histological stains. Efficient antigen retrieval and the Envision-HRP system permit the immunohistological detection of many antigens of diagnostic relevance, with retention of high quality cytomorphology. Furthermore, DNA can be extracted for clonality analysis. The technique can be completed within a similar time period to that of paraffin wax processing with only approximately 30% increase in cost. This technique has been used for diagnosis in over 4000 bone marrow biopsies over the past 14 years. By meeting traditional and contemporary demands on the haematopathologist, it offers a powerful alternative to paraffin wax processing for diagnosis and research.
Krenacs
T
T
Laboratory of Tumour Pathology and Molecular Diagnostics, Institute for Biotechnology, Bay Zoltan Foundation for Applied Research, H-6726 Szeged, Derkovits fasor 2, Hungary. krenacsl@hotmail.com
Bagdi
E
E
Stelkovics
E
E
Bereczki
L
L
Krenacs
L
L
D000941:Antigens / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D001853:Bone Marrow / Q000473:pathology*; D001856:Bone Marrow Examination / Q000379:methods*; D019046:Bone Marrow Neoplasms / Q000175:diagnosis / Q000556:secondary; D004247:DNA / Q000032:analysis; D004853:Epoxy Resins; D015321:Gene Rearrangement; D006402:Hematologic Diseases / Q000175:diagnosis; D006801:Humans; D007124:Immunoenzyme Techniques; D008223:Lymphoma / Q000175:diagnosis; D016612:Paraffin Embedding; D016613:Plastic Embedding / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D013194:Staining and Labeling / Q000379:methods
D000941:Antigens; D015415:Biomarkers; D004853:Epoxy Resins; D004247:DNA