[Structure and function influence of cochlear after dexamethasone intratympanic application].
OBJECTIVE
To study the changes of the structure and function of cochlear after dexamethasone intratympanic application.


METHODS
Forty-five guinea pigs were divided into three groups as normal group, 0.9% sodium chloride injection group and 0.5% dexamethasone group. By using the auditory brainstem response (ABR), the superoxide dismutase (SOD) activity, the nitric oxide (NO) value and cochlear surface preparation technique, the changes of structure and function of cochlear after intratympanic Dexamethasone application has been investigated in this study.


RESULTS
There was not significant difference among three groups in ABR thresholds of wave III (F = 0.5, P = 0.5). There was not significant difference among three groups in SOD activity (F = 2.45, P = 0.96). There was not significant difference among three groups in NO value (F = 3.1, P = 0.3). There was not significant difference among three groups in necrotic values of hair cells on the cochlear surface specimens in 15 mm from basal turn (F = 0.93, P = 0.45).


CONCLUSIONS
There is no change of cochlear construction and function after intratympanic Dexamethasone application.
Liu
Hong-jian
HJ
Department of Otorhinolaryngology, Eye Ear Nose & Throat Hospital, Fudan University, Shanghai 200031, China. lhjent@163.com
Dong
Ming-min
MM
Chi
Fang-lu
FL
D000287:Administration, Topical; D000818:Animals; D003051:Cochlea / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology*; D004432:Ear, Middle / Q000187:drug effects / Q000502:physiology; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects; D006168:Guinea Pigs; D009569:Nitric Oxide / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis
D009569:Nitric Oxide; D003907:Dexamethasone; D013482:Superoxide Dismutase