Serum microarrays for large scale screening of protein levels.
There is a great need for comprehensive proteomic analysis of large patient cohorts of plasma and serum samples to identify biomarkers of human diseases. Here we describe a new antibody-based proteomic approach involving a reverse array format where serum samples are spotted on a microarray. This enables all samples to be screened for their content of a certain serum protein in a single experiment using target-recognizing antibodies and fluorescently labeled secondary antibodies. The procedure is illustrated with the analysis of the IgA levels in 2009 spotted serum samples, and the data are compared with clinical routine measurements. The results suggest that it is possible to simultaneously screen thousands of complex clinical serum samples for their content of the relative amount of specific serum proteins of clinical relevance.
Janzi
Magdalena
M
Division of Clinical Immunology, Karolinska Institute, Karolinska Hospital in Huddinge, SE-14186 Stockholm, Sweden.
Odling
Jenny
J
Pan-Hammarström
Qiang
Q
Sundberg
Mårtenn
M
Lundeberg
Joakim
J
Uhlén
Mathias
M
Hammarström
Lennart
L
Nilsson
Peter
P
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001798:Blood Proteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D046228:Microarray Analysis / Q000379:methods*; D011817:Rabbits; D012680:Sensitivity and Specificity
D000906:Antibodies; D001798:Blood Proteins; D007070:Immunoglobulin A