[Increased expression of peroxisome proliferator-activated receptor-gamma in endothelial cells of spontaneously hypertensive rats].
OBJECTIVE
To evaluate the relationship between hypertension and the expression level of PPAR-gamma in endothelial cells (ECs) of spontaneously hypertensive rats (SHR).


METHODS
PPAR-gamma protein expression in ECs of 4-week and 16-week SHR was detected by immunohistochemistral technique and analyzed by image acquiring and analysis system, and age matched Wistar-Kyoto rats (WKY) were used as controls. The aortic ECs of different-aged rats were primarily cultured, and the protein and mRNA level of PPAR-gamma in cultured ECs (< or =3 passages) were detected by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
The blood pressure of 4-week SHR was slightly higher than those of 4-week WKY (P < 0.05). The protein and mRNA levels of PPAR-gamma in 4-week SHR were also higher than that of 4-week WKY, but the differences had no statistical significance (P > 0.05). Compared with the 16-week WKY, the blood pressure of 16-week SHR greatly increased (P < 0.01), and the protein and mRNA levels of PPAR-gamma in ECs from 16w SHR were approximately 1.5 times higher than those of the age matched WKY (P < 0.01). The protein and mRNA levels of PPAR-gamma in ECs from 16-week SHR were 2.5 times higher than those of 4-week SHR (P < 0.01), while in 16-week WKY it was only 1.5 times higher than those of 4-week WKY (P < 0.01). Conclusion In accordance with the hypertension progress, the protein and mRNA levels of PPAR-gamma in SHR can significantly increase.
Qi
Ying-xin
YX
Department of Cardiology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
Niu
Xiao-lin
XL
Wei
Jin
J
Wang
Nan-ping
NP
D000818:Animals; D001011:Aorta / Q000473:pathology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006973:Hypertension / Q000378:metabolism*; D008297:Male; D047495:PPAR gamma / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D047495:PPAR gamma; D012333:RNA, Messenger