[Roles of endothelin and nitrogen monoxide in the adenine-induced acute renal failure models].
OBJECTIVE
To determine the roles of endothelin (ET) and nitrogen monoxide (NO) in the adenine-induced acute renal failure (ARF) models.


METHODS
ARF models were established by feeding adenine in rats. The activity of NO synthase (NOS) in kidneys of rats was tested by NADPH-diaphorase. Expression of endothelin-1 (ET-1) in kidneys of rats was studied by immunoreactivity method. The area densities for ET-1 and NOS expressions in kidneys were determined by CMM3 pathology image analysis system to calculate the mean light density.


RESULTS
Expressions of ET-1 in normal rat kidneys were wide and weak, but they were greatly enhanced in medulla renis of the ARF models while remained unchanged in the cortex. The activities of NOS were stronger in the cortex and the inner medulla than in the outer medulla. The renal functions of the ARF models were improved after they were treated with L-argine, but the expressions of ET-1 and NOS were not improved. After the withdrawal of adenine, the ARF model rats recovered gradually in their renal functions, and the same was true for the expressions of ET-1 and NOS.


CONCLUSIONS
ET-1 and NO both play important roles in the occurrence, development and recovery of ARF. It is supposed that L-Arg can partly protect the renal functions of ARF rats.
Gui
Ming
M
Department of Nephrology, Third Xiangya Hospital, Central South University, Changsha, China. zkbgm@hotmail.com
Ji
Long-Zhen
LZ
Peng
Chang-Ying
CY
Yang
Jun
J
D058186:Acute Kidney Injury / Q000139:chemically induced / Q000378:metabolism*; D000225:Adenine; D000818:Animals; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000235:genetics; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D019332:Endothelin-1; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D000225:Adenine