[Determination of peimine and peiminine in Fritillaria thunbergii by HPLC-ELSD].
OBJECTIVE
To determine peimine and peiminine in Fritillaria drug simultaneously by RP-HPLC-ELSD.


METHODS
HPLC was carried out with a Waters Alliance, Model 2690, equipped with XTerra RP18 column (150 mm x 3.9 mm ID, 5 microm) and evaporated light scattering detector. The mobile phase (acetonitrile-10 mmol.L(-1) NH4HCO3 adjusted to pH 10.10 by ammonia solution) was eluted in gradient mode.


RESULTS
The recoveries of peimine and peiminine were 98.96% (n = 4), with RSD 1.01% and 98.40% (n = 4), with RSD 2.63%, respectively.


CONCLUSIONS
The method is simple, sensitive and reliable. It can be used for quantitative determination of Fritillaria drug.
Xue
Yan
Y
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. yanxue@imm.ac.cn
Gu
Hao-Liang
HL
D002595:Cevanes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031410:Fritillaria / Q000737:chemistry* / Q000145:classification; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D002595:Cevanes; C014242:verticine; C047331:peiminine