title: Genetic characterization of Trypanosoma cruzi natural clones from the state of Paraíba, Brazil.
abstract: Eighteen Trypanosoma cruzi stocks from the state of Paraíba, Brazil, isolated from man, wild mammals, and triatomine bugs were studied by multilocus enzyme electrophoresis and random primed amplified polymorphic DNA. Despite the low number of stocks, a notable genetic, genotypic, and phylogenetic diversity was recorded. The presence of the two main phylogenetic subdivisions, T. cruzi I and II, was recorded. The strong linkage disequilibrium observed in the population under survey suggests that T. cruzi undergoes predominant clonal evolution in this area too, although this result should be confirmed by a broader sample. The pattern of clonal variation does not suggests a recent origin by founder effect with a limited number of different genotypes.
authors.affiliation: Génétique et Evolution des Maladies Infectieuses, Unité Mixte de Recherche Centre National de la Recherche Scientifique, Institut de Recherche pour le Développement 2724, IRD, BP 64501, 34394 Montpellier Cedex 05, France. Christian.Bernabe@mpl.ird.fr
authors.forename: Christian
authors.initials: C
authors.lastname: Barnabé
authors.forename: M
authors.initials: M
authors.lastname: Tibayrenc
authors.forename: Carlos Brisola
authors.initials: CB
authors.lastname: Marcondes
mesh_terms: D000818:Animals; D001938:Brazil; D002999:Clone Cells; D016054:DNA, Protozoan / Q000032:analysis*; D048293:Didelphis / Q000469:parasitology; D004587:Electrophoresis, Agar Gel; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D014224:Triatoma / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D016054:DNA, Protozoan