Aggrecanase analysis of synovial fluid of temporomandibular joint disorders.
OBJECTIVE
To determine whether or not aggrecanase in synovial fluid can be used as a biochemical marker in the diagnosis of temporomandibular joint disorder (TMJD).


METHODS
Forty-four samples of synovial fluid were obtained from 35 patients with internal derangement or osteoarthritis and 15 control samples from 10 asymptomatic volunteers. Aggrecanase in the synovial fluid was examined by immunoblotting.


RESULTS
The incidence of aggrecanase expression in TMJD group were significantly higher than that in the normal control group (P < 0.05). Those with severe OA and anterior disc displacement without reduction showed significantly high expression of aggrecanase compared with other disease subgroups (P < 0.05).


CONCLUSIONS
These findings suggested that aggrecanase could be a potential biochemical marker for cartilage degeneration in the TMJD.
Yoshida
K
K
Department of Oral and Maxillofacial Surgery, Graduate School of Medical Science, Kanazawa University, Ishikawa, Japan. kany@oral.m.kanazawa-u.ac.jp
Takatsuka
S
S
Tanaka
A
A
Hatada
E
E
Nakamura
H
H
Nakagawa
K
K
Okada
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D010450:Endopeptidases / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D004204:Joint Dislocations / Q000201:enzymology; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology; D013582:Synovial Fluid / Q000201:enzymology*; D013705:Temporomandibular Joint Disorders / Q000201:enzymology*
D015415:Biomarkers; D010450:Endopeptidases; C083641:aggrecanase