title: Vaccinations with T-helper type 1 directing adjuvants have different suppressive effects on the development of allergen-induced T-helper type 2 responses.
abstract: BACKGROUND
  Allergen-induced T-helper type 2 (Th2) responses can be inhibited with Th1 directing vaccines. However, studies comparing the efficacy of the different adjuvants have not been performed in detail.
  
  
  OBJECTIVE
  For this reason we compare the effects of live Bacillus-Calmette-Guerin(BCG), heat-killed (hk)-BCG, CpG-ODN (oligodeoxynucleotide) or PPD on the development of allergen-induced Th2 responses in mice.
  
  
  METHODS
  Ovalbumin (OVA)-specific allergic responses were induced in C57BL/6 mice by two intraperitoneally (i.p.) applications of OVA/alum followed by the intranasal challenge with OVA. The different Th1-inducing adjuvants were applied to the mice together with OVA/alum i.p. during the OVA-sensitization period and, subsequently, different parameters of allergic immune responses were evaluated.
  
  
  RESULTS
  All the adjuvants were effective in inhibiting the development of allergen-induced airway eosinophilia, mucous production and, with the exception of PPD, also airway hyper-reactivity, when they were applied together with OVA/alum. However, allergen-specific IgG1 and IgE serum levels were only reduced in live BCG- and PPD-treated mice. Suppression of airway eosinophilia was not observed in IFN-gamma- or IL-12-deficient mice (hk-BCG, CpG-ODN and PPD). Interestingly, live BCG was still able to suppress allergen-induced Th2 responses in the absence of either IFN-gamma or IL-12. When mice vaccinated with the different adjuvants together with OVA/alum were subjected to a second period of OVA/alum immunization, only live and hk-BCG were able to efficiently suppress the development of airway inflammation. This effect could be adoptively transferred by splenic CD4(+) T cells.
  
  
  CONCLUSIONS
  Taken together our data suggest that live BCG>hk-BCG>CpG-ODN >PPD are effective in suppressing allergen-induced Th2 responses. The degree of suppression and the component of the Th2 response affected (airway inflammation vs. the production of allergen-specific IgE and IgG1) were dependent upon the adjuvant used and how it was applied. Our results contribute to the design of novel vaccines protecting humans from developing allergic disorders.
authors.affiliation: Center for Infectious Diseases, University of Würzburg, Würzburg, Germany. claudia.trujillo@mail.uni-wuerzburg.de
authors.forename: C M
authors.initials: CM
authors.lastname: Trujillo-Vargas
authors.forename: K D
authors.initials: KD
authors.lastname: Mayer
authors.forename: T
authors.initials: T
authors.lastname: Bickert
authors.forename: A
authors.initials: A
authors.lastname: Palmetshofer
authors.forename: S
authors.initials: S
authors.lastname: Grunewald
authors.forename: J R
authors.initials: JR
authors.lastname: Ramirez-Pineda
authors.forename: T
authors.initials: T
authors.lastname: Polte
authors.forename: G
authors.initials: G
authors.lastname: Hansen
authors.forename: G
authors.initials: G
authors.lastname: Wohlleben
authors.forename: K J
authors.initials: KJ
authors.lastname: Erb
mesh_terms: D000276:Adjuvants, Immunologic; D019264:Adoptive Transfer / Q000379:methods; D000485:Allergens / Q000276:immunology*; D000818:Animals; D001500:BCG Vaccine / Q000276:immunology; D002478:Cells, Cultured; D004804:Eosinophils / Q000276:immunology; D005260:Female; D007108:Immune Tolerance / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D012137:Respiratory System / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology; D014611:Vaccination / Q000379:methods*
chemical_list: D000276:Adjuvants, Immunologic; D000485:Allergens; D001500:BCG Vaccine; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D014373:Tuberculin; D018664:Interleukin-12; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin