title: Effect of indole acetic acid administration on the neutrophil functions and oxidative stress from neutrophil, mesenteric lymph node and liver.
abstract: This study was done to investigate the effect of the in vivo administration of indole acetic acid (IAA) on the neutrophil function, the activities of antioxidants enzymes in neutrophils, the mesenteric lymph node and on the oxidative stress in liver and plasma. The animals received subcutaneous administration of IAA in a phosphate-buffered saline (the control group received only the phosphate-buffered saline). The other groups received IAA at concentrations of 1 mg (T1), 2 mg (T2) and 18 mg (T3) per kg of body mass per day. Administration of IAA in both treatments T2 and T3 promoted a significant rise in the phagocytic capacity of neutrophils (by 51%), in comparison with the control. Another alteration was observed in antioxidant enzyme activities of the neutrophil and lymph node. But in the liver, the treatments imposed a significant decrease in the activity of catalase of 19% and 30% for T2 and T3, respectively, in comparison with the control. A similar effect was observed in the activity of hepatic glutathione peroxidase for T3 where a significant decrease of 31%, compared with the control, was obtained. The IAA did not show another significant alteration of the activities of superoxide dismutase and glutathione reductase activities in liver. The hepatic lipid peroxidation level, available by reactive products with thiobarbituric acid, has shown a significant decrease of 27% and 29% with T1 and T3 respectively, in comparison with the control. IAA treatment did not show a significant alteration in reduced glutathione contents in comparison with the liver and plasma controls. In conclusion, the IAA administration has a good potential animal utilization for increasing the phagocytic capacity with no prooxidant effect.
authors.affiliation: Laboratory of Biologic Chemistry, Department of Basic Science, Faculty of Zootechny and Food Engineering (FZEA), University of São Paulo, Pirassununga, Brazil.
authors.forename: Patricia G
authors.initials: PG
authors.lastname: Lins
authors.forename: Claudia R
authors.initials: CR
authors.lastname: Valle
authors.forename: Silvana M P
authors.initials: SM
authors.lastname: Pugine
authors.forename: Diogo L
authors.initials: DL
authors.lastname: Oliveira
authors.forename: Mariana S L
authors.initials: MS
authors.lastname: Ferreira
authors.forename: Ernane J X
authors.initials: EJ
authors.lastname: Costa
authors.forename: Mariza P
authors.initials: MP
authors.lastname: De Melo
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008198:Lymph Nodes / Q000378:metabolism*; D008297:Male; D008643:Mesentery; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007210:Indoleacetic Acids; C030737:indoleacetic acid; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D005978:Glutathione