Expression and characterization of human-elastin-repeat-based temperature-responsive protein polymers for biotechnological purposes.
Rapid progress has been made in the design and synthesis of oligomers and polymers that emulate the properties of natural proteins. Molecular bioengineering offers the chance to design and produce artificial polymeric proteins with tailored polymeric properties. The elastin-like polypeptides are a well-defined family of polymers with noteworthy characteristic based on the VPGVG repeated motif of bovine elastin. In the human homologue, the most regular sequence is represented by the repetition of the VAPGVG hexapeptidic motif. On the basis of this sequence, a synthetic gene has been designed, cloned and expressed in Escherichia coli to obtain artificial protein polymers. The rapid one-step in-frame cloning of any biologically active sequence can be achieved directly in the expression vector, allowing further improvement of the potential of the resulting product.
Bandiera
Antonella
A
Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università degli Studi di Trieste, via Giorgieri 1, 34127 Trieste, Italy. bandiera@bbcm.units.it
Taglienti
Anna
A
Micali
Fulvio
F
Pani
Bianca
B
Tamaro
Marisa
M
Crescenzi
Vittorio
V
Manzini
Giorgio
G
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001704:Biopolymers / Q000737:chemistry*; D001709:Biotechnology; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D004549:Elastin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
D001704:Biopolymers; D020099:Coated Materials, Biocompatible; D011506:Proteins; D011993:Recombinant Fusion Proteins; D004549:Elastin