title: Different expression patterns of duplicated PHANTASTICA-like genes in Lotus japonicus suggest their divergent functions during compound leaf development.
abstract: Recent studies on leaf development demonstrate that the mechanism on the adaxial-abaxial polarity pattern formation could be well conserved among the far-related species, in which PHANTASTICA (PAHN)-like genes play important roles. In this study, we explored the conservation and diversity on functions of PHAN-like genes during the compound leaf development in Lotus japonicus, a papilionoid legume. Two PHAN-like genes in L. japonicus, LjPHANa and LjPHANb, were found to originate from a gene duplication event and displayed different expression patterns during compound leaf development. Two mutants, reduced leaflets1 (rel1) and reduced leaflets3 (rel3), which exhibited decreased adaxial identity of leaflets and reduced leaflet initiation, were identified and investigated. The expression patterns of both LjPHANs in rel mutants were altered and correlated with abnormalities of compound leaves. Our data suggest that LjPHANa and LjPHANb play important but divergent roles in regulating adaxial-abaxial polarity of compound leaves in L. japonicus.
authors.affiliation: National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Shanghai 200032, China.
authors.forename: Jiang Hong
authors.initials: JH
authors.lastname: Luo
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Lin
authors.initials: L
authors.lastname: Weng
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhao
authors.forename: Jiang Hua
authors.initials: JH
authors.lastname: Chen
authors.forename: Xiao He
authors.initials: XH
authors.lastname: Hu
authors.forename: Da
authors.initials: D
authors.lastname: Luo
mesh_terms: D000595:Amino Acid Sequence; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D020131:Genes, Duplicate / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000070116:Lotus / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D017385:Sequence Homology
chemical_list: D018398:Homeodomain Proteins; C405985:Knox1 protein, plant; D010940:Plant Proteins