title: Inhibition of invasion and metastasis in oral cancer by targeting urokinase-type plasminogen activator receptor.
abstract: There have been reports of strong correlations between poor prognosis in various cancers and concomitant expression of urokinase-type plasminogen activator (uPA) and its surface receptor (uPAR). We and others have previously shown that the uPA system plays a significant role in a subset of head and neck squamous cell carcinoma. In the present study, we found that uPAR is required for invasion and metastasis of highly malignant oral cancer cells (OSC-19). Treating OSC-19 cells with antisense oligonucleotides (AS) targeting uPAR resulted in a dramatic decrease of uPAR mRNA expression. Furthermore, pretreatment with AS or siRNA targeting uPAR inhibited progression of OSC-19 cells in experimental models. These results suggest that overexpression of uPAR increases the invasiveness and metastasis of OSC-19 cells, and that uPAR is a promising therapeutic target for regulation of progression of oral cancer.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kanazawa University Graduate School of Medical Science, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8641, Japan. snozaki@med.kanazawa-u.ac.jp
authors.forename: Shinichi
authors.initials: S
authors.lastname: Nozaki
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Endo
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Nakahara
authors.forename: Kunio
authors.initials: K
authors.lastname: Yoshizawa
authors.forename: Yukari
authors.initials: Y
authors.lastname: Hashiba
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kawashiri
authors.forename: Akira
authors.initials: A
authors.lastname: Tanaka
authors.forename: Kiyomasa
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Yudai
authors.initials: Y
authors.lastname: Matsuoka
authors.forename: Mikihiko
authors.initials: M
authors.lastname: Kogo
authors.forename: Etsuhide
authors.initials: E
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000556:secondary; D002642:Chick Embryo; D006801:Humans; D009062:Mouth Neoplasms / Q000201:enzymology* / Q000473:pathology; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D011956:Receptors, Cell Surface / Q000378:metabolism*; D055293:Receptors, Urokinase Plasminogen Activator; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator