Regular arrangement of periodates bound to lysozyme.
The structure of tetragonal hen egg-white lysozyme soaked in a periodate solution has been determined to a resolution of 1.8 A. Four high-occupancy periodate positions have been identified on the basis of the anomalous signal of the I atoms. The four periodates exhibit a regular rectangular arrangement on the surface of the lysozyme molecule. No similar regular arrangement was found either in lysozyme crystals soaked in other heavy-atom anions or in other structures from the Protein Data Bank. Depending on their position on the surface of the protein, the periodate ions deviate to a varying extent from ideal octahedral geometry.
Ondrácek
Jan
J
Department of Recombinant Expression and Structural Biology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo n. 2, CZ-16637 Praha 6, Czech Republic.
Weiss
Manfred S
MS
Brynda
Jirí
J
Fiala
Jaroslav
J
Jursík
Frantisek
F
Rezácová
Pavlína
P
Jenner
Lasse B
LB
Sedlácek
Juraj
J
D000818:Animals; D002645:Chickens; D018360:Crystallography, X-Ray; D004527:Egg Proteins; D005260:Female; D006860:Hydrogen Bonding; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry*; D010504:Periodic Acid / Q000737:chemistry*; D011487:Protein Conformation
D004527:Egg Proteins; D010504:Periodic Acid; C009288:metaperiodate; D009113:Muramidase