title: Modulation of cutaneous wound healing by ozone: differences between young and aged mice.
abstract: Cutaneous tissues are frequently exposed to prooxidative environments, including UV radiation and air pollutants. Among the latter, ozone (O(3)) is of particular concern because of its high and dominating presence in photochemical smog. It is well known that O(3) depletes small molecular weight antioxidants, oxidizes proteins, induces lipid peroxidation and activates cellular responses in various tissues. Using an in vivo model (SKH-1 hairless mice), the interaction between O(3) exposure (0.5ppmx6h/day) and age was examined in relation to cutaneous wound healing. Compared to younger (8 weeks) mice, older (18 months) mice exposed to O(3) (day 0 to day 9 after wounding) exhibited delayed wound closure, increased lipid peroxidation (measured as 4-HNE protein adducts) and protein oxidation (measured as carbonyls concentration) and decreased levels of P-IkappaBalpha and TGFbeta protein. These findings support the hypothesis that oxidant pollutant exposure and age interact so as to disrupt normal wound healing processes.
authors.affiliation: Department of Internal Medicine, School of Medicine, UC Davis, CA, USA.
authors.forename: Yunsook
authors.initials: Y
authors.lastname: Lim
authors.forename: Anh D
authors.initials: AD
authors.lastname: Phung
authors.forename: Ana M
authors.initials: AM
authors.lastname: Corbacho
authors.forename: Hnin Hnin
authors.initials: HH
authors.lastname: Aung
authors.forename: Emanuela
authors.initials: E
authors.lastname: Maioli
authors.forename: Abraham Z
authors.initials: AZ
authors.lastname: Reznick
authors.forename: Carroll E
authors.initials: CE
authors.lastname: Cross
authors.forename: Paul A
authors.initials: PA
authors.lastname: Davis
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Valacchi
mesh_terms: D000375:Aging / Q000502:physiology*; D000447:Aldehydes / Q000032:analysis / Q000737:chemistry; D000818:Animals; D003432:Cross-Linking Reagents / Q000032:analysis / Q000737:chemistry; D005260:Female; D020672:I-kappa B Proteins / Q000096:biosynthesis; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D008812:Mice, Hairless; D023421:Models, Animal; D000072000:NF-KappaB Inhibitor alpha; D010126:Ozone / Q000633:toxicity*; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D012867:Skin / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000447:Aldehydes; D003432:Cross-Linking Reagents; D020672:I-kappa B Proteins; C000606205:Nfkbia protein, mouse; D011506:Proteins; D016212:Transforming Growth Factor beta; D000072000:NF-KappaB Inhibitor alpha; D010126:Ozone; C027576:4-hydroxy-2-nonenal