Comparison of zirconia- and silica-based reversed stationary phases for separation of enkephalins.
In this study, the separation of biologically active peptides on two zirconia-based phases, polybutadiene (PBD)-ZrO2 and polystyrene (PS)-ZrO2, and a silica-based phase C18 was compared. Basic differences in interactions on both types of phases led to quite different selectivity. The retention characteristics were investigated in detail using a variety of organic modifiers, buffers, and temperatures. These parameters affected retention, separation efficiency, resolution and symmetry of peaks. Separation systems consisting of Discovery PBD-Zr column and mobile phase composed of a mixture of acetonitrile and phosphate buffer, pH 2.0 (45:55, v/v) at 70 degrees C and Discovery PS-Zr with acetonitrile and phosphate buffer, pH 3.5 in the same (v/v) ratio at 40 degrees C were suitable for a good resolution of enkephalin related peptides. Mobile phase composed of acetonitrile and phosphate buffer, pH 5.0 (22:78, v/v) was appropriate for separation of enkephalins on Supelcosil C18 stationary phase.
Soukupová
K
K
Department of Analytical Chemistry, Faculty of Science, Charles University, Albertov 2030, 12840 Prague 2, Czech Republic.
Krafková
E
E
Suchánková
Jana
J
Tesarová
E
E
D002021:Buffers; D004745:Enkephalins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature; D015040:Zirconium / Q000737:chemistry*
D002021:Buffers; D004745:Enkephalins; D012822:Silicon Dioxide; D015040:Zirconium; C028541:zirconium oxide