title: [Rapid amplification of 3' cDNA end of Suaeda liaotungensis betaine aldehyde dehydrogenase using one-step PCR].
abstract: Based on part of a known cDNA sequence of Suaeda liaotungensis betaine aldehyde dehydrogenase, we successfully cloned the 3' cDNA end of S. lianotungensis betaine aldehyde dehydrogenase using one-step PCR with a gene-specific primer and universal primer. Compared with the typical 3' RACE, one-step PCR is rapid, simple and inexpensive. It is very rapid to amplify an unknown cDNA 3' end using this method.
authors.affiliation: Biology Department of Liaoning Normal University, Dalian 116029, China.
authors.forename: Qiu-Li
authors.initials: QL
authors.lastname: Li
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Gao
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Yuan
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Liu
authors.forename: Li-Jia
authors.initials: LJ
authors.lastname: An