title: In vitro and intra-macrophage gene expression by Rhodococcus equi strain 103.
abstract: Rhodococcus equi is a facultative intracellular respiratory pathogen of foals that persists and multiplies within macrophages. In foals, virulence is associated with 80-90 kb plasmids, which include a pathogenicity island (PI) containing the virulence-associated protein (vap) gene family, but detailed understanding of the basis of virulence is still poor. A 60 spot-based DNA microarray was developed containing eight PI genes and 42 chromosomal putative virulence or virulence-associated genes selected from a recent partial genome sequence in order to study transcription of these genes by R. equi grown inside macrophages and under in vitro conditions thought to simulate those of macrophages. In addition to seven PI genes, nine chromosomal genes involved in fatty acid and lipid metabolism (choD, fadD13, fbpB), heme biosynthesis (hemE), iron utilization (mbtF), heat shock resistance and genes encoding chaperones (clpB, groEL), a sigma factor (sigK), and a transcriptional regulator (moxR) were significantly induced in R. equi growing inside macrophages. The pattern of R. equi chromosomal genes significantly transcribed inside macrophages largely differed from those transcribed under in vitro conditions (37 degrees C, pH 5.0 or 50mM H2O2 for 30 min). This study has identified genes, other than those of the virulence plasmid, the transcription of which is enhanced within equine macrophages. These genes should be investigated further to improve understanding of how this organism survives intracellularly.
authors.affiliation: Department of Pathobiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Md Tanvir
authors.initials: MT
authors.lastname: Rahman
authors.forename: Valeria
authors.initials: V
authors.lastname: Parreira
authors.forename: John F
authors.initials: JF
authors.lastname: Prescott
mesh_terms: D000193:Actinomycetales Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D000831:Animals, Newborn; D015964:Gene Expression Regulation, Bacterial; D006734:Horse Diseases / Q000276:immunology / Q000382:microbiology*; D006736:Horses; D008264:Macrophages / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D010957:Plasmids / Q000235:genetics*; D012329:RNA, Bacterial / Q000032:analysis; D016929:Rhodococcus equi / Q000235:genetics / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics
chemical_list: D012329:RNA, Bacterial