Expression and characterization of carboxylesterase E4 gene from peach-potato aphid (Myzus persicae) for degradation of carbaryl and malathion.
An abridged carboxylesterase E4 (CbE E4) gene was cloned from the peach-potato aphid, Myzus persicae, by reverse transcription-PCR and subcloned into the expression vector pET28b. The abridged CbE E4 gene was successfully expressed in E. coli BL21 (DE3). The recombinant CbE E4 hydrolyzed beta-naphthyl acetate and Carbaryl by 64% within 2.5 h, Malathion by 80% within 1.25 h. However, the hydrolysis of other pesticides (Dichlorovos, Parathion, Pirimicarb and Deltamethrin) was not detected.
Lan
Wen-sheng
WS
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology Chinese Academy of Sciences, 100080, Beijing, P.R. China.
Cong
Jian
J
Jiang
Hong
H
Jiang
Shu-ren
SR
Qiao
Chuan-ling
CL
D000818:Animals; D001042:Aphids / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D012721:Carbaryl / Q000737:chemistry; D043182:Carboxylesterase / Q000737:chemistry* / Q000235:genetics*; D004247:DNA / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors; D006859:Hydrogen / Q000737:chemistry; D006868:Hydrolysis; D007305:Insecticide Resistance; D008294:Malathion / Q000737:chemistry; D008956:Models, Chemical; D010575:Pesticides / Q000737:chemistry / Q000494:pharmacology; D011994:Recombinant Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D010575:Pesticides; D011994:Recombinant Proteins; D006859:Hydrogen; D004247:DNA; D043182:Carboxylesterase; D012721:Carbaryl; D008294:Malathion