[Establishment of rhesus model for haploidentical hematopoietic stem cell transplantation with nonmyeloablative conditioning].
To study if rhesus haploidentical hematopoietic stem cell transplantation model can be established by non-myeloablative conditioning, parent monkeys were used as donors, offspring monkeys were used as recipients. The recipient monkeys received a nonmyeloablative conditioning consisting of fludarabine, cyclophosphamide, total body irradiation and rabbit anti-human thymocyte globulin. Cyclosporine, mycophenolate mofetil and anti CD25 antibody were used for GVHD prevention. Donor mobilized peripheral blood stem cells were transplantated on day 0. Hematopoietic recovery, chimerism level, GVHD were assessed regularly. The results indicated that hematopoietic recoveries in all 4 cases were observed within 8 days after transplantation. Donor hematopoietic chimerism could be induced in all cases, chimerism analysis showed full donor chimerism (FDC) in case 3 and 4, and II to III grade GVHD developed on day 12 and 14. In case 1, only low level donor chimerism was detected on day 7, and transplantation rejection happened eventually. Unfortunately, kidney failure happened in case 2 after conditioning and died several days later, chimerism analysis showed 50% donor rate on day 7. It is concluded that the rhesus transplantation model was successfully established by nonmyeloablative conditioning for striding over the MHC barrier. This rhesus monkey model would provide a basis for future research.
Liu
Li-Hui
LH
Department of Hematology, The Affiliated Hospital of Academy of Military Medical Sciences, Beijing 10039, China.
Sun
Qi-Yun
QY
Hu
Kai-Xun
KX
Fan
Chuan-Bo
CB
Huang
Ya-Jing
YJ
Bian
Li
L
Xiao
Xiu-Bin
XB
Yao
Bo
B
Guo
Mei
M
Yu
Chang-Lin
CL
Ai
Hui-Sheng
HS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D016572:Cyclosporine / Q000008:administration & dosage; D006086:Graft vs Host Disease / Q000097:blood / Q000209:etiology / Q000517:prevention & control; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D007621:Karyotyping; D008253:Macaca mulatta; D023421:Models, Animal; D009173:Mycophenolic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives; D013997:Time Factors; D018183:Transplantation Chimera / Q000097:blood / Q000235:genetics; D019172:Transplantation Conditioning / Q000379:methods*; D014184:Transplantation, Homologous
D000911:Antibodies, Monoclonal; D053645:Interleukin-2 Receptor alpha Subunit; D016572:Cyclosporine; D009173:Mycophenolic Acid