title: Babesia microti-like parasites detected in feral raccoons (Procyon lotor) captured in Hokkaido, Japan.
abstract: Raccoons (Procyon lotor), which have recently become feral in Japan, were examined for the presence of Babesia microti-like parasites. Out of 372 raccoons captured in the west-central part of Hokkaido, 24 animals with splenomegaly were selected and tested by nested PCR targeting the babesial 18S rRNA gene. B. microti-like parasites were detected in two of the 24 individuals, and their DNA sequences were identical to that of the B. microti-like parasite reported from raccoons in the United States, suggesting that the parasites were probably imported into Japan and that the life cycle of the parasite has already been established in the country. The potential risk of this B. microti-like parasite spreading among dogs and foxes in Japan will need to be carefully monitored, as parasitization by phylogenetically very close parasites has been reported from such animals.
authors.affiliation: School of Veterinary Medicine, Rakuno-Gakuen University, Ebetsu, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Kawabuchi
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Tsuji
authors.forename: Ayako
authors.initials: A
authors.lastname: Sado
authors.forename: Yohei
authors.initials: Y
authors.lastname: Matoba
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Asakawa
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ishihara
mesh_terms: D000818:Animals; D041001:Babesia microti / Q000235:genetics*; D001404:Babesiosis / Q000453:epidemiology* / Q000469:parasitology / Q000662:veterinary*; D002335:Carnivora / Q000097:blood / Q000469:parasitology*; D017931:DNA Primers; D007564:Japan / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013163:Splenomegaly / Q000469:parasitology
chemical_list: D017931:DNA Primers; D012337:RNA, Ribosomal, 18S