title: Metalloprotease gene of Arthroderma gypseum.
abstract: The full-length cDNA sequence for metalloprotease (MEP) of Arthroderma gypseum (one of the teleomorphs of the Microsporum gypseum complex) was determined by the 5'-rapid amplification of cDNA ends (RACE) and 3'-RACE methods using cDNA as a template. The full-length cDNA sequence of the MEP (2,670 bp) gene was proved to encode 677 amino acids. The amino acid sequence of the A. gypseum MEP gene shared about 89 and 66% sequence similarity with the conserved region of the Microsporum canis MEP gene and Aspergillus fumigatus, respectively. Southern hybridization analysis of genomic DNA with an MEP probe gave many distinct bands in BamHI, EcoRI and HindIII digests of genomic DNA from A. gypseum. Reverse transcriptase-PCR analysis suggested that keratin might stimulate the expression of MEP mRNA in A. gypseum.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, Kanagawa 252-8510, Japan. kano@brs.nihon-u.ac.jp
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Koichi
authors.initials: K
authors.lastname: Makimura
authors.forename: Hideyo
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Shinichi
authors.initials: S
authors.lastname: Watanabe
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D000595:Amino Acid Sequence; D003883:Arthrodermataceae / Q000235:genetics* / Q000254:growth & development; D003470:Culture Media; D018076:DNA, Complementary / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007633:Keratins; D045726:Metalloproteases / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D003470:Culture Media; D018076:DNA, Complementary; D007633:Keratins; D045726:Metalloproteases