title: MUC1 and MUC5AC mucin expression in liver fluke-associated intrahepatic cholangiocarcinoma.
abstract: OBJECTIVE
  To investigate the expressions of MUC1 and MUC5AC in intrahepatic cholangiocarcinoma (ICC). Association of expressions of mucins MUC1 and MUC5AC with clinical findings, metastasis, and survival of the liver fluke-associated ICC patients was determined.
  
  
  METHODS
  The expressions of MUC1 and MUC5AC mucins were examined by immunohistochemical staining in 87 cases of histologically-proven ICC. The expressions of mucins in relationship between clinicopathological significance and prognosis of the patients were evaluated.
  
  
  RESULTS
  Fifty-two patients (60%) exhibited both MUC1 and MUC5AC expressions, whereas 31% expressed either MUC1 or MUC5AC, and 9% expressed neither. High MUC1 immunoreactivity displayed a significant correlation with tumor progression as reflected by vascular invasion (P<0.001), whereas high expression of MUC5AC significantly correlated with neural invasion (P = 0.022) and advanced ICC stage (P = 0.008). Patients with high expression of MUC1 had a significantly shorter survival (P = 0.0002). According to multivariate analyses, MUC1 reactivity (P = 0.026), histological grading and stage of tumor represented the least probability of survival.
  
  
  CONCLUSIONS
  MUC1 is overexpressed in liver fluke-associated cholangiocarcinoma and relates to vascular invasion and poor prognosis, whereas MUC5AC mucin is neoexpressed and relates to neural invasion and advanced ICC stage. High MUC1 expression in tumor may be useful for predicting the poor outcome of ICC patients.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Chanchai
authors.initials: C
authors.lastname: Boonla
authors.forename: Banchob
authors.initials: B
authors.lastname: Sripa
authors.forename: Peti
authors.initials: P
authors.lastname: Thuwajit
authors.forename: Ubon
authors.initials: U
authors.lastname: Cha-On
authors.forename: Anucha
authors.initials: A
authors.lastname: Puapairoj
authors.forename: Masanao
authors.initials: M
authors.lastname: Miwa
authors.forename: Sopit
authors.initials: S
authors.lastname: Wongkham
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001650:Bile Duct Neoplasms / Q000401:mortality / Q000469:parasitology* / Q000503:physiopathology; D001653:Bile Ducts, Intrahepatic / Q000469:parasitology* / Q000502:physiology; D014408:Biomarkers, Tumor; D018281:Cholangiocarcinoma / Q000401:mortality / Q000469:parasitology* / Q000503:physiopathology; D005210:Fasciola hepatica; D005211:Fascioliasis / Q000150:complications* / Q000401:mortality / Q000503:physiopathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC; D018396:Mucin-1 / Q000235:genetics* / Q000378:metabolism; D009077:Mucins / Q000235:genetics* / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C116055:MUC1 tandem repeat peptide; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D018396:Mucin-1; D009077:Mucins; D010446:Peptide Fragments