title: [Methodical approaches to obtaining of isolated cardiomyocytes].
abstract: Isolated cardiac cells represent a unique model system for the study of many aspects of myocardial function. In this paper, several procedures for isolation ofmyocytes from adult animals and human hearts are described and discussed. They are based on successive treatments with Ca(2+)-free medium, disaggregating enzymes and/or mechanical agiatation.
authors.forename: M V
authors.initials: MV
authors.lastname: Egorova
authors.forename: S A
authors.initials: SA
authors.lastname: afanas'ev
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D006801:Humans; D009206:Myocardium / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology; D051381:Rats