Effects of endothelin-1 on melanocyte adhesion and migration.
OBJECTIVE
To investigate the effect of endothelin-1 (ET-1) on the adhesion and migration of melanocyte in vitro.


METHODS
Human epidermal melanocytes that had been cultured and purified were treated with ET-1 and observed for adhesion to bovine serum fibronectin-coated culture dishes. Stem cell factor (SCF) and ET-1 treated cells were also examined for migration into micropore filters coated with the same protein.


RESULTS
Compared with the SCF group, ET-1 treated melanocytes were easier to adhere to the dishes and to be moved into the filters, especially when the concentration was 32 nmol/L. When the concentration of ET-1 was 128 nmol/L or more, it could inhibit the adhesion and migration of melanocyte. At any concentration of ET-1 except at 2 nmol/L, there was a significant difference between ET-1-treated and untreated melanocytes (P < 0.01) when the adhesion test was carried out. However, even at 2 nmol/ L ET-1, there was obviously increased migration compared with those of ET-1 untreated melanocytes and SCF-treated cells (P < 0.01).


CONCLUSIONS
ET-1 may influence the adhesion and migration of melanocyte, which can partly explain the capacity of ET-1 to regulate the melanocyte function in vitiligo lesions.
Mou
Kuan-Hou
KH
Department of Dermatology, First Hospital of Xi'an Jiaotong University, Xi'an, China. moukuanhou@sohu.com
Zhang
Xian-Qi
XQ
Yu
Bing
B
Zhang
Zhi-Ling
ZL
Feng
Jie
J
D002448:Cell Adhesion / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D019332:Endothelin-1 / Q000494:pharmacology*; D006801:Humans; D008544:Melanocytes / Q000166:cytology*; D012867:Skin / Q000166:cytology; D014820:Vitiligo / Q000628:therapy
D019332:Endothelin-1