Expression of the Medicago truncatula DM12 gene suggests roles of the symbiotic nodulation receptor kinase in nodules and during early nodule development.
The Medicago truncatula DMI2 gene encodes a receptorlike kinase required for establishing root endosymbioses. The DMI2 gene was shown to be expressed much more highly in roots and nodules than in leaves and stems. In roots, its expression was not altered by nitrogen starvation or treatment with lipochitooligosaccharidic Nod factors. Moreover, the DMI2 mRNA abundance in roots of the nfp, dmil, dmi3, nsp1, nsp2, and hcl symbiotic mutants was similar to the wild type, whereas lower levels in some dmi2 mutants could be explained by regulation by the nonsense-mediated decay, RNA surveillance mechanism. Using pDMI2::GUS fusions, the expression of DMI2 in roots appeared to be localized primarily in the cortical and epidermal cells of the younger, lateral roots and was not observed in the root apices. Following inoculation with Sinorhizobium meliloti, the DMI2 gene was induced in the nodule primordia, before penetration by the infection threads. No increased expression was seen in lateral-root primordia. In nodules, expression was observed primarily in a few cell layers of the pre-infection zone. These results are consistent with the DMI2 gene mediating Nod factor perception and transduction leading to rhizobial infection, not only in root epidermal cells but also during nodule development.
Bersoult
Anne
A
Laboratoire des Interactions Plantes-Microorganismes, CNRS-INRA, BP52627, 31326 Castanet-Tolosan Cedex, France.
Camut
Sylvie
S
Perhald
Ariana
A
Kereszt
Attila
A
Kiss
György B
GB
Cullimore
Julie V
JV
D018506:Gene Expression Regulation, Plant; D046913:Medicago truncatula / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology* / Q000378:metabolism* / Q000382:microbiology; D021381:Protein Transport; D012333:RNA, Messenger / Q000378:metabolism; D013559:Symbiosis
D010940:Plant Proteins; D012333:RNA, Messenger