Extracellular production of a glycolipid biosurfactant, mannosylerythritol lipid, by Candida sp. SY16 using fed-batch fermentation.
Candida sp. strain SY16 produces a glycolipid-type biosurfactant, mannosylerythritol lipid (MEL-SY16), which can reduce the surface tension of a culture broth from 72 to 30 dyne cm(-1) and highly emulsify hydrocarbons when cultured in soybean-oil-containing media. As such, laboratory-scale fermentation for MEL-SY16 production was performed using optimized conditions. In batch fermentation, MEL-SY16 was mainly produced during the stationary phase of growth, and the concentration of MEL-SY16 reached 37 g l(-1) after 200 h. The effect of pH control on the production of MEL-SY16 was also examined in batch fermentation. The highest production yield of MEL-SY16 was when the pH was controlled at 4.0, and the production was significantly improved compared to batch fermentation without pH control. In fed-batch fermentation, glucose and soybean oil (1:1, w/w) were used in combination as the initial carbon sources for cell growth, and soybean oil was used as the feeding carbon source during the MEL production phase. The feeding of soybean oil resulted in the disappearance of any foam and a sharp increase in the MEL production until 200 h, at which point the concentration of MEL-SY16 was 95 g l(-1). Among the investigated culture systems, the highest MEL-SY16 production and volumetric production rate were achieved with fed-batch fermentation.
Kim
Hee-Sik
HS
Environmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yusong, Daejon, South Korea.
Jeon
Jong-Woon
JW
Kim
Byung-Hyuk
BH
Ahn
Chi-Yong
CY
Oh
Hee-Mock
HM
Yoon
Byung-Dae
BD
D002175:Candida / Q000378:metabolism*; D005285:Fermentation; D006017:Glycolipids / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D013501:Surface-Active Agents / Q000378:metabolism*
D006017:Glycolipids; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid