Surface plasmon resonance: applications in understanding receptor-ligand interaction.
During last decade there has been significant progress in the development of analytical techniques for evaluation of receptor-ligand interaction. Surface plasmon resonance (SPR)-based optical biosensors are now being used extensively to define the kinetics of wide variety of macromolecular interactions and high- and low-affinity small molecule interactions. The experimental design data analysis methods are evolving along with widespread applications in ligand fishing, microbiology, virology, host-pathogen interaction, epitope mapping, and protein-, cell-, membrane-, nucleic acid-protein interactions. SPR-based biosensors have strong impact on basic and applied research significantly. This brief review describes the SPR technology and few of its applications in relation to receptor-ligand interaction that has brought significant change in the methodology, analysis, interpretation, and application of the SPR technology.
Pattnaik
Priyabrata
P
Virology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002 India. ppat@rediffmail.com
D015374:Biosensing Techniques; D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods
D008024:Ligands