Eukaryotic membrane protein overproduction in Lactococcus lactis.
Eukaryotic membrane proteins play many vital roles in the cell and are important drug targets. Approximately 25% of all genes identified in the genome are known to encode membrane proteins, but the vast majority have no assigned function. Although the generation of structures of soluble proteins has entered the high-throughput stage, for eukaryotic membrane proteins only a dozen high-resolution structures have been obtained so far. One major bottleneck for the functional and structural characterisation of membrane proteins is the overproduction of biologically active material. Recent advances in the development of the Lactococcus lactis expression system have opened the way for the high-throughput functional expression of eukaryotic membrane proteins.
Kunji
Edmund R S
ER
The Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Wellcome Trust/MRC Building, Cambridge CB2 2XY, United Kingdom. ek@mrc-dunn.cam.ac.uk
Chan
Ka Wai
KW
Slotboom
Dirk Jan
DJ
Floyd
Suzanne
S
O'Connor
Rosemary
R
Monné
Magnus
M
D000818:Animals; D001419:Bacteria / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005057:Eukaryotic Cells / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D013294:Lactococcus lactis / Q000235:genetics*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000235:genetics; D000226:Mitochondrial ADP, ATP Translocases / Q000096:biosynthesis / Q000235:genetics; D018000:Receptors, Peptide / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics
C064459:KDEL receptor; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C112225:PET9 protein, S cerevisiae; D018000:Receptors, Peptide; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D000226:Mitochondrial ADP, ATP Translocases