title: Protein-protein interaction map of the Trypanosoma cruzi ribosomal P protein complex.
abstract: The large subunit of the eukaryotic ribosome possesses a long and protruding stalk formed by the ribosomal P proteins. Four out of five ribosomal P proteins of Trypanosoma cruzi, TcP0, TcP1alpha, TcP2alpha, and TcP2beta had been previously characterized. Data mining of the T. cruzi genome data base allowed the identification of the fifth member of this protein group, a novel P1 protein, named P1beta. To gain insight into the assembly of the stalk, a yeast two-hybrid based protein interaction map was generated. A parasite specific profile of interactions amongst the ribosomal P proteins of T. cruzi was evident. The TcP0 protein was able to interact with all both P1 and both P2 proteins. Moreover, the interactions between P2beta with P1alpha as well as with P2alpha were detected, as well as the ability of TcP2beta to homodimerize. A quantitative evaluation of the interactions established that the strongest interacting pair was TcP0-TcP1beta.
authors.affiliation: Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECH), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Centro de Genómica Aplicada (CeGA), Universidad de Buenos Aires, Argentina.
authors.forename: Maximiliano
authors.initials: M
authors.lastname: Juri Ayub
authors.forename: Cristian R
authors.initials: CR
authors.lastname: Smulski
authors.forename: Benson
authors.initials: B
authors.lastname: Nyambega
authors.forename: Natalia
authors.initials: N
authors.lastname: Bercovich
authors.forename: Dan
authors.initials: D
authors.lastname: Masiga
authors.forename: Martín P
authors.initials: MP
authors.lastname: Vazquez
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Aguilar
authors.forename: Mariano J
authors.initials: MJ
authors.lastname: Levin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019281:Dimerization; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000235:genetics* / Q000378:metabolism; D010449:Peptide Mapping / Q000379:methods; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D012269:Ribosomal Proteins; D014349:Trypanosoma cruzi / Q000235:genetics*; D020798:Two-Hybrid System Techniques
chemical_list: D046912:Multiprotein Complexes; D010750:Phosphoproteins; D015800:Protozoan Proteins; D012269:Ribosomal Proteins; C035690:ribosomal phosphoprotein P1