[Depression of mPges expression by troglitazone in human renal glomerulus induced by high glucose and LPS].
OBJECTIVE
To determine the influence of troglitanzone on the expression of mPGEs induced by high glucose or LPS in human glomeruli cultured in vitro.


METHODS
Human glomeruli were isolated and incubated by LPS or high glucose absence or presence with different concentrations of troglitazone. The concentration of mPGEs in the supernatant was measured by an enzyme-linked immunosorbent assay (ELISA).


RESULTS
LPS (10 microg/ml) and 5% glucose could improve the concentration of mPGEs significantly in the supernatant by cultured human glomeruli, and troglitazone (10 - 20 nmol/L) could block its increase (P < 0.05).


CONCLUSIONS
High glucose and LPS can improve mPGEs expression in cultured human glomeruli, and troglitazone can block this effects. These data suggest that PPAR-gamma agonist troglitanzone may be relevant to the treatment of diabetic nephropathy and inflammation in glomerluli.
Ling
Guang-Hui
GH
Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha 410011 , China.
Peng
You-Ming
YM
Wang
Hai-Tao
HT
Duan
Shao-Bing
SB
Liu
Ying-Hong
YH
Liu
Fu-You
FY
D001786:Blood Glucose / Q000378:metabolism*; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology*; D003864:Depression, Chemical; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D007678:Kidney Glomerulus / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D047495:PPAR gamma / Q000819:agonists; D000071876:Prostaglandin-E Synthases; D045162:Thiazolidinediones / Q000494:pharmacology*; D000077288:Troglitazone
D001786:Blood Glucose; D002839:Chromans; D008070:Lipopolysaccharides; D047495:PPAR gamma; D045162:Thiazolidinediones; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D000077288:Troglitazone