Buccal DNA samples for DNA typing: new collection and processing methods.
With the recent expansion of DNA database laws in many states, there is a critical need for the rapid and simple collection of DNA samples and streamlined processing for downstream applications. The Buccal DNA Collector was developed to address the need for a reliable, practical alternative to blood collection that is compatible with high-throughput operations. The collection area consists of filter paper that is placed against the inside of the cheek, and the sample is taken by swiping the cheek several times while pulling the device out of the mouth. Using this method, DNA profiles have been obtained from samples stored for 2 years at room temperature. Cells are collected on all regions of the filter paper with the maximum DNA recovery from the tip. The processing of DNA for DNA typing is accomplished with BodeElute, a new product that prepares DNA for amplification in a single 30-min heating step. Extracted DNA samples were successfully amplified with four commonly used multiplex short tandem repeat (STR) amplification kits. These products provide simplified approaches for collecting and processing buccal cell samples.
Burger
Martha Frantz
MF
The Bode Technology Group Inc., Springfield, VA 22150, USA.
Song
Eun Young
EY
Schumm
James W
JW
D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000295:instrumentation / Q000379:methods*; D005554:Forensic Medicine / Q000295:instrumentation / Q000379:methods; D005838:Genotype; D006801:Humans; D009061:Mouth Mucosa / Q000737:chemistry*; D011933:Reagent Kits, Diagnostic; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods*
D011933:Reagent Kits, Diagnostic; D004247:DNA