title: Differential distribution of voltage-gated calcium channel alpha-2 delta (alpha2delta) subunit mRNA-containing cells in the rat central nervous system and the dorsal root ganglia.
abstract: Voltage-gated calcium channels (VGCCs) play an essential role in controlling neurotransmitter release, neuronal excitability, and gene expression in the nervous system. The distribution of cells that contain mRNAs encoding the auxiliary alpha2delta-1, alpha2delta-2, and alpha2delta-3 subunits of the VGCCs in the central nervous system (CNS) and the dorsal root ganglia (DRG) was examined in rats by using in situ hybridization. Specific labeling of alpha2delta-1, alpha2delta-2, and alpha2delta-3 mRNAs appeared to be largely confined to neurons and was widely, although differentially, distributed in the brain, the spinal cord, and the DRG. Importantly, alpha2delta-2 mRNA was found to be expressed in interneurons in the cortex, the hippocampus, the striatum, and in regions that contain dense cholinergic neurons. Our results suggest that different alpha2delta subunits may exert distinctive functions in the CNS. The alpha2delta-1 subunit mRNA is localized in brain regions known to be involved in cortical processing, learning and memory, defensive behavior, neuroendocrine secretion, autonomic activation, primary sensory transmission, and general arousal. The alpha2delta-2 subunit mRNA is present in brain regions known to modulate the overall activities of the cortex, the hippocampus, and the thalamus. The alpha2delta-2 subunit is also found in brain regions known to be involved in olfaction, somatic motor control, fluid homeostasis, ingestive and defensive behaviors, neuroendocrine functions, and circadian rhythm. In addition to being localized in brain regions that express alpha2delta-1 and alpha2delta-2 subunit mRNAs, alpha2delta-3 subunit mRNA is highly expressed in regions involved in auditory information processing and somatic movement.
authors.affiliation: Department of Pharmacology, Merck Research Laboratories, San Diego, California 92037, USA.
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Cole
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Lechner
authors.forename: Mark E
authors.initials: ME
authors.lastname: Williams
authors.forename: Pat
authors.initials: P
authors.lastname: Prodanovich
authors.forename: Leo
authors.initials: L
authors.lastname: Bleicher
authors.forename: Mark A
authors.initials: MA
authors.lastname: Varney
authors.forename: Guibao
authors.initials: G
authors.lastname: Gu
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D002490:Central Nervous System / Q000166:cytology*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D016000:Cluster Analysis; D005727:Ganglia, Spinal / Q000166:cytology*; D015870:Gene Expression / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D009474:Neurons / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015220:Calcium Channels; D010320:Parvalbumins; D021122:Protein Subunits; D012333:RNA, Messenger; D002795:Choline O-Acetyltransferase