Identification of the testis-specific protein 10 (TSGA10) as serologically defined tumour-associated antigen in primary cutaneous T-cell lymphoma.
BACKGROUND
The number of identified tumour-associated antigens for cutaneous lymphoma is still very restricted, which limits the elucidation of the tumour immunology of these malignancies and the development of specific immunotherapies and immunodiagnostics.


OBJECTIVE
To identify new serologically defined antigens associated with cutaneous lymphoma.


METHODS
A phage expression library of the human testis transcriptom was established and immunoscreened with sera from 100 patients with cutaneous lymphoma and nine with parapsoriasis, and 81 age-matched control donors. Positive expression clones were sequenced to identify the respective antigen.


RESULTS
The testis-specific protein 10 (TSGA10) was identified as an antigen recognized by sera of two patients with Mycosis fungoides but not by sera from healthy donors. By reverse transcription-polymerase chain reaction analysis, TSGA10 was found expressed in all cutaneous lymphoma samples tested, various tumour cell lines, testis, peripheral blood mononuclear cells, skin, isolated lymphocytes, keratinocytes and fibroblasts. TSGA10 overexpression had previously been reported for other cancers.


CONCLUSIONS
TSGA10 is a new tumour-associated antigen of cutaneous lymphoma.
Theinert
S M
SM
Department of Dermatology, Venereology and Allergy, Charité-Universitaetsmedizin Berlin, Humboldt University, D-10098 Berlin, Germany.
Pronest
M M
MM
Peris
K
K
Sterry
W
W
Walden
P
P
D020121:5' Untranslated Regions; D000368:Aged; D000951:Antigens, Neoplasm / Q000097:blood*; D016022:Case-Control Studies; D003598:Cytoskeletal Proteins; D004274:DNA, Recombinant / Q000032:analysis; D005260:Female; D015723:Gene Library; D006801:Humans; D016410:Lymphoma, T-Cell, Cutaneous / Q000276:immunology*; D008297:Male; D009182:Mycosis Fungoides / Q000097:blood; D010267:Parapsoriasis / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000032:analysis*; D012878:Skin Neoplasms / Q000276:immunology*; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution
D020121:5' Untranslated Regions; D000951:Antigens, Neoplasm; D003598:Cytoskeletal Proteins; D004274:DNA, Recombinant; D011506:Proteins; C429756:TSGA10 protein, human