Age associated changes in erythrocyte membrane surface charge: Modulatory role of grape seed proanthocyanidins.
Aging, a multifactorial process of enormous complexity is characterized by impairment of physio-chemical and biological aspects of cellular functions. It is closely associated with increased free radical production, which situation ultimately leads to devastation of normal cell function and membrane integrity. The present study was aimed to determine the effect of proanthocyanidins rich grape seed extract (GSP) on membrane surface charge density in erythrocytes during animal age associated oxidative stress. GSP (100 mg/day/kg body weight) was administered orally for 15 and 30 days to young and aged rats. Significant decrease in surface charge levels with concomitant increase in protein carbonyls and decrease in glycoprotein, antioxidants status was noted in erythrocytes of aged rats when compared with young rat erythrocytes. Duration dependent supplementation of GSP increased the erythrocyte surface charge density to near normalcy in aged rats. Decrease in protein carbonyls level and increase in glycoproteins as well as antioxidant status was observed in aged rat erythrocytes on GSP treatment. Thus, from our results, we conclude that GSP is an effective anti-aging drug in preventing the oxidative stress associated loss of membrane surface charge, which thereby maintains the erythrocyte membrane integrity and functions in elderly.
Sangeetha
Purushotham
P
Department of Medical Biochemistry, Dr AL Mudaliar Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, India.
Balu
Muthaiya
M
Haripriya
Dayalan
D
Panneerselvam
Chinnakannu
C
D000375:Aging / Q000502:physiology*; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000097:blood; D002374:Catalase / Q000032:analysis; D004910:Erythrocyte Membrane / Q000502:physiology*; D005978:Glutathione / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000032:analysis; D027843:Vitis
D000975:Antioxidants; D006023:Glycoproteins; D010936:Plant Extracts; D044945:Proanthocyanidins; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid