Genietic and molecular characterization of Candystripel transposition events in sorghum.
In sorghum, the Candystripe1 (Cs1) transposable element causes a variegated pericarp phenotype due to its excision activity from the yl (yellow seed1) locus. The Y1 is a transcription regulator which is required for the biosynthesis of red 3-deoxyflavonoid pigments. Somatic variability in the transposition behavior of Cs1 was observed via biochemical analysis of 3-deoxyflavonoids in the leaf tissues of the Y1-cs alleles. Using somatic excisions of Cs1 as a tool, we establish that the Cs1 is active in young seedlings and the y1 locus is also functional in these tissues. Several somatic and germinal excision events were characterized and sequence analysis of independent events predominantly showed 2-bp footprints. Further, with the goal of ur.derstanding the properties of Cs1 that would facilitate the development of a transposon tagging system in sorghum, germinal excisions of Cs1 from y1 were used as a marker. Transposition of Cs1 was followed by characterization of putative insertion events. Genetic linkage between mutant phenotypes and the cosegregating restriction fragments of Cs1 provided additional evidence that Cs1 is an active transposable element in sorghum.
Carvalho
Carlos H S
CH
Embrapa, Varginha, MG 37026-400 Brazil
Boddu
Jayanand
J
Zehr
Usha B
UB
Axtell
John D
JD
Pedersen
Jeffery F
JF
Chopra
Surinder
S
D001483:Base Sequence; D003433:Crosses, Genetic; D004251:DNA Transposable Elements / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D005419:Flavonoids / Q000096:biosynthesis; D017343:Genes, Plant; D009154:Mutation; D010641:Phenotype; D045868:Sorghum / Q000235:genetics* / Q000378:metabolism
D004251:DNA Transposable Elements; D018744:DNA, Plant; D005419:Flavonoids