title: Distribution of P2Y2 receptors in the guinea pig enteric nervous system and its coexistence with P2X2 and P2X3 receptors, neuropeptide Y, nitric oxide synthase and calretinin.
abstract: The distribution of P2Y2 receptor-immunoreactive (ir) neurons and fibers and coexistence of P2Y2 with P2X2 and P2X3 receptors, neuropeptide Y (NPY), calretinin (CR), calbindin (CB) and nitric oxide synthase (NOS) was investigated with immunostaining methods. The results showed that P2Y2-ir neurons and fibers were distributed widely in myenteric and submucous plexuses of the guinea pig stomach corpus, jejunum, ileum and colon. The typical morphology of P2Y2-ir neurons was a long process with strong positive staining on the same side of the cell body. The P2Y2-ir neurons could be Dogiel type 1. About 40-60% P2X3-ir neurons were immunoreactive for P2Y2 in the myenteric plexus and all the P2X3-ir neurons expressed the P2Y2 receptor in the submucosal plexus; almost all the NPY-ir neurons and the majority of CR-ir neurons were also immunoreactive for P2Y2, especially in the myenteric plexus of the small intestine; no P2Y2-ir neurons were immunoreactive for P2X2 receptors, CB and NOS. It is shown for the first time that S type/Dogiel type 1 neurons with fast P2X and slow P2Y receptor-mediated depolarizations could be those neurons expressing both P2Y2-ir and P2X3-ir and that they are widely distributed in myenteric and submucosal plexuses of guinea pig gut.
authors.affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, 200433, Shanghai, People's Republic of China.
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Xiang
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Burnstock
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D064032:Calbindin 2; D019084:Fluorescent Antibody Technique, Indirect; D006168:Guinea Pigs; D007124:Immunoenzyme Techniques; D009197:Myenteric Plexus / Q000166:cytology / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D058476:Receptors, Purinergic P2X2; D058477:Receptors, Purinergic P2X3; D058473:Receptors, Purinergic P2Y2; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013368:Submucous Plexus / Q000166:cytology / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D064032:Calbindin 2; D009478:Neuropeptide Y; D018048:Receptors, Purinergic P2; D058476:Receptors, Purinergic P2X2; D058477:Receptors, Purinergic P2X3; D058473:Receptors, Purinergic P2Y2; D064030:S100 Calcium Binding Protein G; D052248:Nitric Oxide Synthase Type I