Lifestyle intervention and fatty acid metabolism in glucose-intolerant subjects.
OBJECTIVE
Free fatty acid (FFA) oxidation is reduced in subjects with type 2 diabetes mellitus and impaired glucose tolerance (IGT). Weight reduction does not improve these impairments. Because exercise training is known to increase fatty acid (FA) oxidation, we investigated whether a combined diet and physical activity intervention program can improve FA oxidation in subjects with IGT.


METHODS
Sixteen subjects with IGT were studied before and after 1 year of a lifestyle intervention program [nine intervention (INT) subjects, seven controls (CON)]. INT subjects received regular (i.e., every 3 months) dietary advice and were stimulated to increase their level of physical activity. Glucose tolerance, anthropometric characteristics, and substrate use at rest and during exercise were evaluated before and after 1 year. Substrate oxidation was measured at rest and during moderate intensity exercise using indirect calorimetry in combination with stable isotope infusion ([U-(13)C]palmitate and [6,6-(2)H(2)-]glucose).


RESULTS
After 1 year, no differences were seen in substrate use at rest. During exercise, total fat and plasma FFA oxidation were slightly increased in the INT group and decreased in the CON group, with the change being significantly different (change after 1 year: INT, +2.0 +/- 1.4 and +1.9 +/- 0.9 micromol/kg per minute; CON, -3.5 +/- 1.6 and -1.8 +/- 0.5 micromol/kg per minute for total and plasma FFA, respectively; p < 0.05).


CONCLUSIONS
A combined diet and physical activity intervention program can prevent further deterioration of impaired FA oxidation during exercise in subjects with IGT.
Mensink
Marco
M
Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht, Maastricht University, The Netherlands. m.mensink@hb.unimaas.nl
Blaak
Ellen E
EE
Wagenmakers
Anton J
AJ
Saris
Wim H
WH
D003924:Diabetes Mellitus, Type 2 / Q000178:diet therapy / Q000378:metabolism* / Q000628:therapy*; D004734:Energy Metabolism; D005081:Exercise Therapy; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D008019:Life Style; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction
D005227:Fatty Acids