[Study on fingerprint chromatograms of water-soluble constituents of Salvia miltiorrhiza Bge. by high performance liquid chromatography].
The fingerprint chromatograms were established for the quality evaluation of Salvia miltiorrhiza Bge. by high performance liquid chromatography. The analysis was performed on a Zorbax SB-C18 column (5 microm, 4.6 mm i.d. x 250 mm) with acetonitrile-water (containing 0.4% (v/v) acetic acid ) (the volume fraction of acetonitrile from 0 to 40% within 70 min) as mobile phase at a flow rate of 1.0 mL/min, and at a column temperature of 25 degrees C. The detection wavelength was 254 nm. The relative standard deviations of relative retention values and relative peak areas were less than 3%. The mutual fingerprint of the extract of Salvia miltiorrhiza Bge. collected from Danshen Base in Tongren City of Guizhou Province was established by using the similarity calculation software of Chinese herbal fingerprint. The method is reliable and can be helpful in effectively controlling the quality of Salvia miltiorrhiza Bge.
Zhou
Xin
X
The Key Laboratory of Chemistry for Natural Products of Guizhou Province and The Chinese Academy of Sciences, Guiyang 550002, China. alice9800@sina.com
Wang
Daoping
D
Liang
Guangyi
G
Zhang
Xueqin
X
Zhang
Zhonglin
Z
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D010618:Phenanthrolines / Q000032:analysis*; D027561:Salvia miltiorrhiza / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D010618:Phenanthrolines; C028972:dan-shen root extract