title: Up- and downregulated genes in muscles that undergo developmentally programmed cell death in the insect Manduca sexta.
abstract: This study was designed to investigate changes in gene expression associated with stage-specific programmed cell death (PCD) in intersegmental muscles (ISMs) of the moth, Manduca sexta. The technique of differential display reverse transcription PCR was applied to compare mRNA levels before and after the onset of PCD in ISMs. Expression of E75B transcription factor was repressed while another factor, betaFTZ-F1, stayed at a very low level. However, gene coding for a translation-initiation factor (eIF1A) was upregulated. Expression of these genes had not been previously reported to be altered in dying ISMs. An ecdysteroid agonist, RH-5849, that prevented PCD in ISMs also blocked these changes.
authors.affiliation: Department of Anatomy, Cell and Developmental Biology, Loránd Eötvös University, Budapest, Pázmány Péter sétány 1/C., H-1117, Hungary. peterlow@cerberus.elte.hu
authors.forename: Péter
authors.initials: P
authors.lastname: Löw
authors.forename: Gábor Csaba
authors.initials: GC
authors.lastname: Talián
authors.forename: Miklós
authors.initials: M
authors.lastname: Sass
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D026461:Ecdysteroids / Q000037:antagonists & inhibitors; D015977:Eukaryotic Initiation Factor-1 / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006834:Hydrazines / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D018402:Manduca / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D026461:Ecdysteroids; D015977:Eukaryotic Initiation Factor-1; D006834:Hydrazines; D019476:Insect Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C026022:eukaryotic peptide initiation factor-1A; C056565:1,2-dibenzoyl-tert-butylhydrazine