Forensic identification of urine samples: a comparison between nuclear and mitochondrial DNA markers.
Urine samples from 20 male volunteers of European Caucasian origin were stored at 4 degrees C over a 4-month period in order to compare the identification potential of nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) markers. The amount of nDNA recovered from urines dramatically declined over time. Consequently, nDNA likelihood ratios (LRs) greater than 1,000 were obtained for 100, 70 and 55% of the urines analysed after 6, 60 and 120 days, respectively. For the mtDNA, HVI and HVII sequences were obtained for all samples tested, whatever the period considered. Nevertheless, the highest mtDNA LR of 435 was relatively low compared to its nDNA equivalent. Indeed, LRs obtained with only three nDNA loci could easily exceed this value and are quite easier to obtain. Overall, the joint use of nDNA and mtDNA markers enabled the 20 urine samples to be identified, even after the 4-month period.
Castella
V
V
Laboratoire de Génétique Forensique, Institut Universitaire de Médecine Légale, rue du Bugnon 21, 1005 Lausanne, Switzerland. Vincent.Castella@chuv.ch
Dimo-Simonin
N
N
Brandt-Casadevall
C
C
Robinson
N
N
Saugy
M
M
Taroni
F
F
Mangin
P
P
D002467:Cell Nucleus / Q000235:genetics*; D022801:Complementarity Determining Regions / Q000235:genetics; D004272:DNA, Mitochondrial / Q000652:urine*; D053803:Forensic Genetics; D005819:Genetic Markers; D006801:Humans; D016013:Likelihood Functions; D008297:Male; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013048:Specimen Handling; D013997:Time Factors; D044465:White People / Q000235:genetics
D022801:Complementarity Determining Regions; D004272:DNA, Mitochondrial; D005819:Genetic Markers