Hydroxyapatite/tricalcium phosphate matrix scaffold as cell carriers in vitro.
OBJECTIVE
To explore the possibility of hydroxyapatite/tricalcium phosphate (HA/TCP) matrix scaffold as cell carriers in tissue engineering, and to provide a direct evidence for the resurfacing of matrix scaffold.


METHODS
Human bone marrow stromal cells (hBMSCs) and umbilical vein endothelial cells (UVECs) were co-cultured on the surface of matrix scaffold. The morphologic characters during culture on HA/TCP matrix scaffold's surfaces were checked by light microscope, fluorescence microscope, and scanning electronic microscope.


RESULTS
Both human bone marrow stromal cells and human umbilical vein endothelial cells showed good biocompatibility with HA/TCP matrix scaffold. The cell growth into the micropore of HA/TCP matrix scaffold could be seen under both fluorescence microscopy and scaning electron microscopy.


CONCLUSIONS
HA/TCP matrix scaffold can be used as a vehicle for the cell transplantation.
Mao
Xin-Zhan
XZ
Department of Orthopedics, Second Xiangya Hospital, Central South University, Changsha, China. docmxz@sina.com
Zhou
Jiang-Nan
JN
Hu
Jian-Zhong
JZ
Ruan
Jian-Min
JM
Wang
Wan-Chun
WC
Ni
Jiang-Dong
JD
D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology*; D002130:Calcium Phosphates; D017690:Cell Transplantation; D002478:Cells, Cultured; D017886:Durapatite; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D017154:Stromal Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods; D014471:Umbilical Veins / Q000166:cytology
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D017886:Durapatite; C018392:tricalcium phosphate