[Effects of an antioxidant (An7845) on the proliferation and apoptosis of K562 leukemia cells].
OBJECTIVE
To determine the effects of an antioxidant (An7845) on the proliferation inhibition and apoptosis induction of a human leukemia cell line (K562).


METHODS
We evaluated the effects of An7845 on K562 cell proliferation by cellular enumeration in liquid culture assay, MTT assay and colony formation assay. We also verified the effects of An7845 on K562 cell apoptosis by cellular morphology and the DNA fragments appeared in agarose gel electrophoresis.


RESULTS
The proliferation of K562 leukemia cells was inhibited by An7845 (5 x 10(-8) - 5 x 10(5) mol/L) in a dose-dependent manner. K562 cells were induced toward apoptosis by An7845 (5 x 10(-7) mol/L).


CONCLUSIONS
An7845 can effectively inhibit the proliferation and induce the apoptosis of K562 leukemia cells.
Song
Kui
K
Research Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China. hqun888@tom.com
He
Qun
Q
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020014:K562 Cells
D000975:Antioxidants