title: Modelling of ceramide interactions with porous graphite carbon in non-aqueous liquid chromatography.
abstract: Interactions of solutes on porous graphitic carbon (PGC) with non-aqueous mobile phases are studied by the linear solvation energy relationship (LSER). Studies have been carried out with eight binary mixtures composed of a weak solvent (acetonitrile or methanol) and a strong solvent (tetrahydrofuran, n-butanol, CH2Cl2, 1,1,2-trichloro-2,2,1-trifluoroethane). The systematic analysis of a set of test compounds was performed for each solvent mixture in isocratic mode (50:50). The results were compared to those obtained on PGC with hydro-organic liquids and supercritical fluids. They were then correlated with the observed retention behaviour of lipid compounds, more particularly ceramides.
authors.affiliation: LETIAM, University de Paris-Sud (XI), IUT d'Orsay, Plateau du Moulon, F-91400 Orsay, France.
authors.forename: C
authors.initials: C
authors.lastname: West
authors.forename: G
authors.initials: G
authors.lastname: Cilpa
authors.forename: K
authors.initials: K
authors.lastname: Gaudin
authors.forename: P
authors.initials: P
authors.lastname: Chaminade
authors.forename: E
authors.initials: E
authors.lastname: Lesellier
mesh_terms: D002518:Ceramides / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006108:Graphite / Q000737:chemistry*
chemical_list: D002518:Ceramides; D006108:Graphite