Hepatotoxic effects of tert-butyl hydroperoxide (t-BHP) and protection by antioxidants.
t-BHP induced oxidative stress and Ca2+ function impairment in fresh hepatocytes was studied in order to understand its role in cytotoxicity. Viability of hepatocytes by the release of lactate dehydrogenase and methyl thiazoletetrazolium reduction method alongwith malondialdehyde formation indicated oxidative stress in the hepatotoxic action of t-BHP.
Singh
Sangram
S
Department of Biochemistry, Dr. R. M. L. Avadh University, Faizabad 224 001, India.
Mehrotra
Sudhir
S
Pandey
Rajeev
R
Sandhir
Rajat
R
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
D000975:Antioxidants; D008054:Lipid Peroxides; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide