Live, attenuated Salmonella typhimurium vectoring Campylobacter antigens.
We describe the evaluation of three live, attenuated deltaphoP/Q Salmonella enteric serovar Typhimurium strains expressing PEB1 minus its signal sequence (PEB1-ss) from three different plasmids: a pBR-based asd plasmid, an arabinose-based runaway plasmid, which each expressed PEB1-ss in the bacterial cytosol, and a PEB1::HlyA fusion plasmid that directs secretion of PEB1-ss into the extracellular milieu. Serum IgG responses specific for PEB1-ss were induced by pBR-derived and runaway plasmids, with 100 and 90% seroconversion, respectively, at a 1:500 dilution of anti-sera as measured by Western blot analysis, while the PEB1-ss::HlyA fusion plasmid induced serum IgG in only 20% of the mice. Although significant levels of anti-PEB serum IgG were induced, no protection against oral Campylobacter jejuni challenge was observed.
Sizemore
Donata R
DR
Battelle Memorial Institute, Columbus, OH 43201, USA. sizemored@battelle.org
Warner
Beth
B
Lawrence
Julie
J
Jones
Amy
A
Killeen
Kevin P
KP
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D002169:Campylobacter Infections / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D016123:Campylobacter jejuni / Q000276:immunology*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D005260:Female; D005822:Genetic Vectors; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D021382:Protein Sorting Signals / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D006460:Hemolysin Proteins; D007074:Immunoglobulin G; C489977:PEB1 protein, Campylobacter; D021382:Protein Sorting Signals; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic