A fluorogenic and chromogenic probe that detects the esterase activity of trace copper(II).
Bis(picolinoyl)fluorescein, a new fluorogenic and chromogenic probe for monitoring the esterase activity of metal ions, has been synthesized. The probe is highly selective for Cu2+ and is applicable both to quantification of the free ion and detection of esterolytic activity of Cu bound to organic ligands or biomolecules, with a detection limit of 100 nM by fluorimetry.
Kierat
Radoslaw M
RM
Anorganisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany.
Krämer
Roland
R
D002863:Chromogenic Compounds; D003300:Copper / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D005456:Fluorescent Dyes; D006868:Hydrolysis; D007700:Kinetics; D008024:Ligands; D015335:Molecular Probes; D013329:Structure-Activity Relationship
D002863:Chromogenic Compounds; D005456:Fluorescent Dyes; D008024:Ligands; D015335:Molecular Probes; D003300:Copper; D004950:Esterases