title: Fluorescent nanocrystals for use in early cervical cancer detection.
abstract: BACKGROUND
  Quantum dots (qdots) are a promising alternative to organic fluorophores for biological imaging. Advantages of quantum dots over organic fluorophores include broad excitation coupled with narrow, tunable emission, high resistance to chemical and metabolic degradation, a higher photobleaching threshold and finally the ability to be modified with a targeting ligand. These many properties allow quantum dots to be used in conjunction with optical detection methods for imaging.
  
  
  METHODS
  We are investigating the use of quantum dots to detect precancerous biomarkers. We have directly targeted epidermal growth factor receptors with quantum dots conjugated to anti-EGFR antibodies.
  
  
  RESULTS
  Compared to appropriate controls, we do see specific labeling of EGF receptors.
  
  
  CONCLUSIONS
  Quantum dots provide a promising alternative to conventional organic dyes for biological imaging. Combined with optical imaging technologies, quantum dots can help visualize changes in cervical cancer at the molecular level. This ability may alert health care providers to the need for intervention before a cancer can metastasize.
authors.affiliation: Optical Imaging and Spectroscopy Lab, The University of Texas at Austin, 2501 Speedway, ENS 10, Austin, TX 78712, USA. dawn.nida@engr.utexas.edu
authors.forename: Dawn L
authors.initials: DL
authors.lastname: Nida
authors.forename: Mohammed S
authors.initials: MS
authors.lastname: Rahman
authors.forename: Kristen D
authors.initials: KD
authors.lastname: Carlson
authors.forename: Rebecca
authors.initials: R
authors.lastname: Richards-Kortum
authors.forename: Michelle
authors.initials: M
authors.lastname: Follen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D051379:Mice; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000378:metabolism; D045663:Quantum Dots; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D066246:ErbB Receptors