Comparison of three different solid-phase microextraction fibres for analysis of essential oils in yacon (Smallanthus sonchifolius) leaves.
A headspace solid-phase microextraction (HS-SPME) procedure based on three commercialised fibers (100 microm polydimethylsiloxane, 65 microm polydimethylsiloxane-divinylbenzene and 50/30 microm divinylbenzene-Carboxen-polydimethylsiloxane) is presented for the determination of a selected essential oils in dried leaves of yacon (Smallanthus sonchifolius). The extraction performances of these compounds were compared using fibers with one, two and three coatings. The optimal experimental procedures for the adsorption and desorption of target compounds were determined. Significant parameters affecting sorption process such as sample weight, sorption and desorption time and temperature were optimised and discussed. Finally, the optimised procedures were applied successfully for the determination of these compounds in various yacon species. The relative concentration factors of three characteristic components of yacon were measured for relative evaluation of the fiber efficiency. Main essential oils were isolated from dried yacon leaves by appropriate solid-phase microextraction fiber and semi-quantitative analysis of the target volatiles was conducted by gas chromatography-flame ionisation detection (GC-FID) using a capillary column. Three compounds--beta-pinene, caryophylene and y-cadinene were found as the predominant essential oils. Its relative content was important for specification of yacon varieties. Solid-phase microextraction in combination with gas chromatography enabled a rapid and simple determination of relative content of essential oils in yacon.
Adam
M
M
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, nam. Cs. legii 565, 532 10 Pardubice, Czech Republic. martin.adam@upce.cz
Juklová
M
M
Bajer
T
T
Eisner
A
A
Ventura
K
K
D019659:Asteraceae / Q000737:chemistry*; D005591:Chemical Fractionation / Q000295:instrumentation* / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods*; D004129:Dimethylpolysiloxanes; D009822:Oils, Volatile / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry*; D010938:Plant Oils / Q000032:analysis*; D010945:Plants, Edible / Q000737:chemistry; D011145:Polyvinyls; D012828:Silicones; D013696:Temperature
D004129:Dimethylpolysiloxanes; D009822:Oils, Volatile; D010938:Plant Oils; D011145:Polyvinyls; D012828:Silicones; C445089:poly(dimethylsiloxane-divinylbenzene); C013830:baysilon