Nucleoplasm staining patterns and cell cycle-associated expression of Ki-67 in middle ear cholesteatoma.
OBJECTIVE
To compare Ki-67 expression patterns in middle ear cholesteatoma with the corresponding retroauricular and external auditory canal skins, and to determine the cell cycle-dependent localization of Ki-67.


METHODS
MIB-1 monoclonal antibody was used for comparative assessment of proliferative activity of middle ear cholesteatoma, external auditory canal skin, and retroauricular skin samples on formalin-fixed paraffin-embedded tissue sections. Primary keratinocytes from cholesteatoma tissue were isolated and subjected to kinetic analysis of the cell cycle.


RESULTS
Higher proliferative activity was established in cholesteatoma in comparison with retroauricular and external auditory canal skins. Three different staining patterns have been described. Kinetic analysis revealed continuous expression of Ki-67 during all active phases of the cell cycle and remained "silent" in resting cells.


CONCLUSIONS
The established correlation between the staining patterns and cell cycle-associated expression of Ki-67 specifies Ki-67 as a reliable and stable marker of proliferation for middle ear cholesteatoma.
Raynov
Alexander M
AM
Department of Otorhinolaryngology, University Hospital Tzaritza Joanna, Medical University-Sofia, Sofia, Bulgaria.
Moon
Sung-Kyun
SK
Choung
Yun-Hoon
YH
Hong
Sung Pyo
SP
Park
Keehyun
K
D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism*; D004424:Ear Canal / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism*; D051379:Mice; D013194:Staining and Labeling; D014432:Tympanic Membrane / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D019394:Ki-67 Antigen