title: 7-hydroxy-3-(4-hydroxybenzyl)chroman and broussonin b: neurotrophic compounds, isolated from Anemarrhena asphodeloides BUNGE, function as proteasome inhibitors.
abstract: The extract of Anemarrhenae Rhizoma (rhizomes of Anemarrhena asphodeloides BUNGE) showed neurotrophic activity toward rat pheochromocytoma (PC-12) cells. Bioassay-guided purification afforded four compounds, 2,6,4'-trihydroxy-4-methoxybenzophenone (1), 7-hydroxy-3-(4-hydroxybenzyl)chroman (2), broussonin B (3), and cis-hinokiresinol (4). Compounds 1-3 induced neurite outgrowth in PC-12 cells at concentration of 50 microg/ml, while 4 was less active. In addition, compounds 2-4 showed moderate inhibitory activities against a chymotrypsin-like activity of the proteasome.
authors.affiliation: Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan. sachiko@p.kanazawa-u.ac.jp
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tsukamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Wakana
authors.forename: Keiichirou
authors.initials: K
authors.lastname: Koimaru
authors.forename: Takushi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Mako
authors.initials: M
authors.lastname: Sato
authors.forename: Tomihisa
authors.initials: T
authors.lastname: Ohta
mesh_terms: D000473:Alkanes / Q000494:pharmacology*; D031403:Anemarrhena / Q000737:chemistry*; D000818:Animals; D001593:Benzyl Compounds / Q000494:pharmacology*; D002491:Central Nervous System Agents / Q000494:pharmacology*; D002839:Chromans / Q000494:pharmacology*; D002918:Chymotrypsin / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D009420:Nervous System / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects; D016716:PC12 Cells; D010636:Phenols / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011480:Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats
chemical_list: C504759:7-hydroxy-3-(4-hydroxybenzyl)chroman; D000473:Alkanes; D001593:Benzyl Compounds; D002491:Central Nervous System Agents; D002839:Chromans; D010636:Phenols; D011480:Protease Inhibitors; C504760:broussonin B; D002918:Chymotrypsin