title: Mediation of neurogenic ion transport by acetylcholine, prostanoids and 5-hydroxytryptamine in porcine ileum.
abstract: Enteric neural activity modulates active transepithelial ion transport in the intestine. We investigated the neural circuits mediating neurogenic secretion in mucosal explants from porcine ileum. Transmural electrical stimulation increased short-circuit current, a measure of active ion transport, by 35+/-2 microA/cm2. The neuronal Na+ channel blocker saxitoxin, the muscarinic cholinergic receptor antagonist atropine, the 5-hydroxytryptamine3 receptor antagonist tropisetron, and the cyclooxygenase inhibitor indomethacin inhibited this response. In addition, tropisetron inhibited the atropine-resistant portion of the response, and both atropine and indomethacin attenuated the saxitoxin-resistant component. Neurogenic secretion in porcine ileum appears to be mediated by tryptaminergic and prostanoid-sensitive cholinergic pathways.
authors.affiliation: Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, MN 55108-6010, USA.
authors.forename: DeWayne
authors.initials: D
authors.lastname: Townsend
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Casey
authors.forename: David R
authors.initials: DR
authors.lastname: Brown
mesh_terms: D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004558:Electric Stimulation; D005260:Female; D007082:Ileum / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000294:innervation / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects; D008297:Male; D011453:Prostaglandins / Q000494:pharmacology*; D012530:Saxitoxin / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology*; D018490:Serotonin Agents / Q000494:pharmacology; D012702:Serotonin Antagonists / Q000494:pharmacology; D013552:Swine; D000077526:Tropisetron; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D007211:Indoles; D011453:Prostaglandins; D018490:Serotonin Agents; D012702:Serotonin Antagonists; D014665:Vasodilator Agents; D012701:Serotonin; D012530:Saxitoxin; C025421:eicosa-8,11,14-trien-5-ynoic acid; D000077526:Tropisetron; D001285:Atropine; D015126:8,11,14-Eicosatrienoic Acid; D000109:Acetylcholine; D007213:Indomethacin