Over-representation of bcr3 subtype of PML/RARalpha fusion gene in APL in Indian patients.
Thirty six patients with acute promyelocytic leukemia were studied by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR. There was concordance between the results achieved by both the methods except in one case, which was negative by RT-PCR but positive by real-time PCR. The prevalence of bcr3 (short isoform) was found to be significantly higher than that of bcr1 (long isoform) (64 vs. 36%, P=0.03). No correlation was found between age, sex, and white blood cell (WBC) count at diagnosis. Molecular remission was achieved in 66.6% of patients with bcr3 isoform. Median WBC count at presentation was found to be higher than that in the West.
Sazawal
Sudha
S
Department of Haematology, All India Institute of Medical Sciences, New Delhi, 110 029, India.
Hasan
Syed Khizer
SK
Dutta
Pankhi
P
Kumar
Bijender
B
Kumar
Rajat
R
Kumar
Lalit
L
Choudhry
V P
VP
Saxena
Renu
R
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007194:India; D015473:Leukemia, Promyelocytic, Acute / Q000097:blood* / Q000175:diagnosis; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D015514:Oncogene Proteins, Fusion / Q000096:biosynthesis*; D015995:Prevalence; D020033:Protein Isoforms / Q000096:biosynthesis; D051562:Proto-Oncogene Proteins c-bcr / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D020033:Protein Isoforms; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein; D051562:Proto-Oncogene Proteins c-bcr