Humoral immune response to recombinant viral NS3 protein in patients with hepatitis C.
Full-length recombinant NS3 protein was used in a test system for detection of specific antibodies in the sera from patients with hepatitis C. Possible antigenic determinants in NS structure were predicted. It was demonstrated that serological analysis requires enzyme immunoassay with full-length NS3.
Gudim
E A
EA
Institute of Transplantology and Artificial Organs, Moscow, Russia. elenagdm@yandex.ru
Agapov
I I
II
Komolov
I S
IS
Tonevitskii
A G
AG
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases