title: The proteome of maritime pine wood forming tissue.
abstract: Wood is one of our most important natural resources. Surprisingly, we know hardly anything about the details of the process of wood formation. The aim of this work was to describe the main proteins expressed in wood forming tissue of a conifer species (Pinus pinaster Ait.). Using high resolution 2-DE with linear pH gradient ranging from 4 to 7, a total of 1039 spots were detected. Out of the 240 spots analyzed by MS/MS, 67.9% were identified, 16.7% presented no homology in the databases, and 15.4% corresponded to protein mixtures. Out of the 57 spots analyzed by MALDI-MS, only 15.8% were identified. Most of the 175 identified proteins play a role in either defense (19.4%), carbohydrates (16.6%) and amino acid (14.9%) metabolisms, genes and proteins expression (13.1%), cytoskeleton (8%), cell wall biosynthesis (5.7%), secondary (5.1%) and primary (4%) metabolisms. A summary of the identified proteins, their putative functions, and behavior in different types of wood are presented. This information was introduced into the PROTICdb database and is accessible at http://cbib1.cbib.u-bordeaux2.fr/Protic/Protic/home/index.php. Finally, the average protein amount was compared with their respective transcript abundance as quantified through EST counting in a cDNA-library constructed with mRNA extracted from wood forming tissue.
authors.affiliation: UMR 1202 BIOGECO, INRA, Equipe de Génétique, Cestas, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Gion
authors.forename: Céline
authors.initials: C
authors.lastname: Lalanne
authors.forename: Grégoire
authors.initials: G
authors.lastname: Le Provost
authors.forename: Hélène
authors.initials: H
authors.lastname: Ferry-Dumazet
authors.forename: Jorge
authors.initials: J
authors.lastname: Paiva
authors.forename: Phillipe
authors.initials: P
authors.lastname: Chaumeil
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Frigerio
authors.forename: Jean
authors.initials: J
authors.lastname: Brach
authors.forename: Aurélien
authors.initials: A
authors.lastname: Barré
authors.forename: Antoine
authors.initials: A
authors.lastname: de Daruvar
authors.forename: Stéphane
authors.initials: S
authors.lastname: Claverol
authors.forename: Marc
authors.initials: M
authors.lastname: Bonneu
authors.forename: Nicolas
authors.initials: N
authors.lastname: Sommerer
authors.forename: Luc
authors.initials: L
authors.lastname: Negroni
authors.forename: Christophe
authors.initials: C
authors.lastname: Plomion
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D020224:Expressed Sequence Tags; D028223:Pinus; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020543:Proteome; D012333:RNA, Messenger / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014934:Wood
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012333:RNA, Messenger