[The structure of uncommon lipid A from the marine bacterium Marinomonas communis ATCC 27118T].
Lipid A was obtained in a high yield (27%) by the hydrolysis of lipopolysaccharide from the marine gamma proteobacterium Marinomonas communis ATCC 27118T with 1% AcOH. Using chemical analysis and ID and 2D NMR spectroscopic and fast atom bombardment mass spectrometric methods, it was shown to be beta-(1',6)-linked D-glucosaminobiose 1-phosphate acylated with (R)-3-dodecanoyl- or (R)-3-decanoyloxydecanoic acid, (R)-3-[(R)-3-hydroxydecanoyloxy)]decanoic acid, and (R)-3-hydroxydecanoic acid at the C2, C2' and C3 positions, respectively. Uncommon structural peculiarities (a low acylation and phosphorylation degree) of the M. communis lipid A in comparison with those of terrestrial bacteria may be of pharmacological interest. The potential physiological meaning of this lipid A and compounds of similar structure are discussed.
Vorob'eva
E V
EV
Dmitrenok
A S
AS
Dmitrenok
P S
PS
Isakov
V V
VV
Krasikova
I N
IN
Solov'eva
T F
TF
D002850:Chromatography, Gel; D008050:Lipid A / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D044130:Oceanospirillaceae / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D008050:Lipid A