title: Expression analysis of immune-related genes in CD34(+) progenitor-derived dendritic cells after exposure to the chemical contact allergen DNCB.
abstract: We studied the changes in gene expression after exposure of human dendritic cells (DCs) to the model allergen dinitrochlorobenzene (DNCB). DCs were derived from CD34(+) progenitor cells of three different donors and exposed to 10 microM DNCB or solvent for several time intervals (3, 6 and 12h). cDNA microarrays were used to assess the transcriptional activity of 11,000 human genes. Compared to control gene expression, changes larger than +/-two-fold were observed for 241 genes after exposure to DNCB. Of these genes, 137 were up-regulated and 104 down-regulated. Twenty of these genes encode proteins that are related to the immune response (cytokines, chemokines, their receptors, cytokine/chemokines-related genes, transcription and signal transduction genes) and are discussed in more detail. Our data indicate that exposure to DNCB does not induce a typical maturation pattern in DCs.
authors.affiliation: Vito (Flemish Institute for Technological Research), Centre of Expertise in Environmental Toxicology, Boeretang 200, 2400 Mol, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Schoeters
authors.forename: G R
authors.initials: GR
authors.lastname: Verheyen
authors.forename: R
authors.initials: R
authors.lastname: Van Den Heuvel
authors.forename: I
authors.initials: I
authors.lastname: Nelissen
authors.forename: H
authors.initials: H
authors.lastname: Witters
authors.forename: V F I
authors.initials: VF
authors.lastname: Van Tendeloo
authors.forename: G E R
authors.initials: GE
authors.lastname: Schoeters
authors.forename: Z N
authors.initials: ZN
authors.lastname: Berneman
mesh_terms: D000485:Allergens / Q000633:toxicity*; D018952:Antigens, CD34 / Q000032:analysis / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D018925:Chemokines / Q000096:biosynthesis / Q000235:genetics; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D004137:Dinitrochlorobenzene / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000235:genetics; D013997:Time Factors
chemical_list: D000485:Allergens; D018952:Antigens, CD34; D018925:Chemokines; D016207:Cytokines; D004137:Dinitrochlorobenzene; D019707:Receptors, Chemokine