title: Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454).
abstract: A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP.
authors.affiliation: Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Jun-ichiro
authors.initials: J
authors.lastname: Sekiguchi
authors.forename: Prasit
authors.initials: P
authors.lastname: Tharavichitkul
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.forename: Vena
authors.initials: V
authors.lastname: Chupia
authors.forename: Tomoko
authors.initials: T
authors.lastname: Fujino
authors.forename: Minako
authors.initials: M
authors.lastname: Araake
authors.forename: Atsushi
authors.initials: A
authors.lastname: Irie
authors.forename: Koji
authors.initials: K
authors.lastname: Morita
authors.forename: Tadatoshi
authors.initials: T
authors.lastname: Kuratsuji
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
mesh_terms: D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000494:pharmacology*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003428:Cross Infection / Q000382:microbiology*; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005493:Folic Acid Antagonists / Q000494:pharmacology*; D006801:Humans; D016106:Methicillin Resistance; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D014295:Trimethoprim / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D003470:Culture Media; D005493:Folic Acid Antagonists; D014295:Trimethoprim; D013762:Tetrahydrofolate Dehydrogenase