title: Microarray analysis of toxicogenomic effects of peracetic acid on Pseudomonas aeruginosa.
abstract: Hospital-acquired (nosocomial) infection (HAI) represents a serious threat to public health, both in terms of human casualty and in terms of economic impact. On an annual basis, 2 million individuals require prolonged hospitalization, and an estimated 90 000 patients die due to HAI. Economic damages are reported to exceed $4.5 billion, annually. While many disinfectants, including peracetic acid, have been employed to eradicate infectious bacteria, a lack of understanding their mode of action and the corresponding defense mechanisms hinders successful antimicrobial application. We report here the first transcriptome analysis of the response of Pseudomonas aeruginosa, a pathogen infecting those with cystic fibrosis, upon 20 min exposure to a sublethal concentration (1 mM) of peracetic acid. As a result, we identified that 570 out of a total of 5570 P. aeruginosa genes showed statistically significant transcript level changes. Our findings indicate that (i) many genes associated with cellular protective processes were induced, (ii) the transcription of genes involved in primary metabolic pathways was repressed, and (iii) the transcription of genes encoding membrane proteins and small molecule transporters was altered. We also observed that genes within operons were highly cotranscribed in this study. Finally, this global transcriptional profile can help identify signature genes that are also activated with other oxidative disinfectants, which may be used to design new more effective treatments or more efficaciously apply existing compounds.
authors.affiliation: Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, Maryland 20742, USA.
authors.forename: Wook
authors.initials: W
authors.lastname: Chang
authors.forename: David A
authors.initials: DA
authors.lastname: Small
authors.forename: Freshteh
authors.initials: F
authors.lastname: Toghrol
authors.forename: William E
authors.initials: WE
authors.lastname: Bentley
mesh_terms: D004202:Disinfectants / Q000633:toxicity*; D005798:Genes, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D010463:Peracetic Acid / Q000633:toxicity*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D043922:Toxicogenetics; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D004202:Disinfectants; D010463:Peracetic Acid