Neuron numbers in sensory cortices of five delphinids compared to a physeterid, the pygmy sperm whale.
With its large mass and enormous gyrification, the neocortex of whales and dolphins has always been a challenge to neurobiologists. Here we analyse the relationship between neuron number per cortical unit in three different sensory areas and brain mass in six different toothed whale species, five delphinids and one physeterid. Cortex samples, including primary cortical areas of the auditory, visual, and somatosensory systems were taken from both hemispheres of brains fixed in 10% buffered formalin. The samples were embedded in paraffin, sectioned at 25 microm thickness and stained with cresyl violet. Because cortical thickness varies among toothed whale species, cell counts were done in cortical units measuring 150mum in width, 25 microm in thickness, and extending from the pial surface to the white matter. By arranging the delphinid brains according to their total mass, 834-6052 g, we found decreasing neuron numbers in the investigated areas with increasing brain mass. The pigmy sperm whale (Kogia breviceps), a physeterid with an adult brain weight of 1000 g had a distinctly lower neuron number per cortical unit. As had been expected, an increase in adult brain weight in delphinid cetaceans (family Delphinidae) is not correlated with an increase in neuron number per cortical unit.
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Department of Anatomy (III), Johann Wolfgang Goethe University, Frankfurt aM, Germany. spindoctor_c@web.de
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Güntürkün
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Ridgway
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Oelschläger
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D000818:Animals; D048588:Benzoxazines; D002452:Cell Count / Q000706:statistics & numerical data; D002540:Cerebral Cortex / Q000166:cytology*; D004289:Dolphins / Q000033:anatomy & histology*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009474:Neurons / Q000166:cytology*; D009929:Organ Size / Q000502:physiology; D010078:Oxazines; D014907:Whales / Q000033:anatomy & histology*
D048588:Benzoxazines; D005904:Glial Fibrillary Acidic Protein; D010078:Oxazines; C028911:cresyl violet