title: Fluorous affinity purification of oligonucleotides.
abstract: [structure: see text] Nucleoside phosphoramidites bearing a fluorous dimethoxytrityl (FDMT) group were used to synthesize fluorous-tagged oligonucleotides, which were subjected to solid-phase extraction using a pH-stable fluorinated adsorbent. On-column detritylation afforded the purified oligonucleotides. The fluorous affinity purification method offers one-pass loading without ammonia removal, high selectivity for the removal of failure sequences, high recoveries (typically 70-100%), and the ability to purify long oligonucleotides (e.g., 50-100-mers).
authors.affiliation: Berry & Associates, Inc., 2434 Bishop Circle East, Dexter, Michigan 48130, USA. wpearson@berryassoc.com
authors.forename: William H
authors.initials: WH
authors.lastname: Pearson
authors.forename: David A
authors.initials: DA
authors.lastname: Berry
authors.forename: Patrick
authors.initials: P
authors.lastname: Stoy
authors.forename: Kee-Yong
authors.initials: KY
authors.lastname: Jung
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Sercel
mesh_terms: D000345:Affinity Labels / Q000737:chemistry*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D005461:Fluorine / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000302:isolation & purification*
chemical_list: D000345:Affinity Labels; D009841:Oligonucleotides; D005461:Fluorine