Simple and quick chemical aminoacylation of tRNA in cationic micellar solution under ultrasonic agitation.
A simple and quick method for direct aminoacylation of a tRNA with a non-natural amino acid was developed by using an N-protected amino acid cyanomethyl ester as a substrate solubilized in CTACl micelle under ultrasonic agitation.
Hashimoto
Naoto
N
Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama, 700-8530, Japan. sisido@cc.okayama-u.ac.jp.
Ninomiya
Keiko
K
Endo
Takamasa
T
Sisido
Masahiko
M
D000241:Adenosine / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry*; D015153:Blotting, Western; D002412:Cations; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000737:chemistry; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008823:Micelles; D012333:RNA, Messenger / Q000096:biosynthesis / Q000737:chemistry; D012343:RNA, Transfer / Q000737:chemistry*; D012996:Solutions; D019809:Streptavidin / Q000096:biosynthesis; D014465:Ultrasonics
D000596:Amino Acids; D002412:Cations; D007202:Indicators and Reagents; D008823:Micelles; D012333:RNA, Messenger; D012996:Solutions; D019809:Streptavidin; D012343:RNA, Transfer; D000241:Adenosine