Transient ischemia-induced changes of neurofilament 200 kDa immunoreactivity and protein content in the main olfactory bulb in gerbils.
This study was carried out to investigate alterations of neurofilament 200 kDa (NF-200) and its polyphosphorylation form (RT97) immunoreactivity and protein content in the main olfactory bulb (MOB) after 5 min of transient forebrain ischemia in gerbils. In the sham-operated group, weak NF-200 immunoreactivity was detectable in a few somata of mitral cells, which projected weak NF-200-immunoreactive processes to the external plexiform layer (EPL). At 1-5 days after ischemia, strong NF-200 and RT97 immunoreactivity was shown by the mitral cell processes; however, somata of mitral cells did not show NF-200 immunoreactivity. At this time point, strong NF-200-immunoreactive mitral cell processes ran to the EPL and glomerular layer (GL). Thereafter, NF-200 and RT97 immunoreactivity was decreased up to 30 days after ischemia. In the 15 days post-ischemic group, the distribution pattern of NF-200 and RT97 immunoreactivity was slightly lower than that in the 1-5 days post-ischemic groups. In the 30 days post-ischemic group, moderate NF-200 and RT97 immunoreactivity was found in the mitral cells processes, but the immunoreactivity in the EPL and GL nearly disappeared. A Western blot study showed a pattern of NF-200 and RT97 expression at all post-ischemic time points similar to that of immunohistochemistry after ischemia. This result indicates that NF-200 and RT97 accumulates in injured mitral cell processes a few days after transient ischemia, which suggests that the axonal transport in the MOB may be disturbed during this period after transient ischemia.
Hwang
In Koo
IK
Department of Anatomy, College of Medicine, Hallym University, Chunchon 200-702, South Korea.
Koh
Un-San
US
Lee
Jae Chul
JC
Yoo
Ki-Yeon
KY
Song
Ji-Hyun
JH
Jung
Ju-Young
JY
Nam
Young Sam
YS
Lee
In Se
IS
Kang
Tae-Cheon
TC
Won
Moo Ho
MH
D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D001369:Axons / Q000276:immunology / Q000473:pathology; D015153:Blotting, Western; D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D003599:Cytoskeleton / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005849:Gerbillinae; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D016900:Neurofilament Proteins / Q000378:metabolism*; D009474:Neurons / Q000276:immunology / Q000473:pathology; D019581:Neuropil / Q000276:immunology / Q000473:pathology; D009830:Olfactory Bulb / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009833:Olfactory Pathways / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D010766:Phosphorylation; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D013997:Time Factors
D016900:Neurofilament Proteins; D011506:Proteins; C051774:neurofilament protein H