[Construction of cell lines producing high-titer recombinant retroviruses of message and antisense cDNA of human glucocorticoid receptor beta].
OBJECTIVE
To construct cell lines producing high-titer recombinant retroviruses of human glucocorticoid receptor beta (hGRbeta).


METHODS
The recombinant retrorinal vectors of message and antisense cDNA of hGRbeta were transfected into PA317 cells by liposomes, respectively, and cell colonies were selected with G418. The titers of viruses from the 2 cell culture supernatants were determined by colony numbers produced in NIH3T3 cells.


RESULTS
The presence of aimed RNAs in supernatant of the G418-resistant cell cultures were detected by RT-PCR and subsequent sequence analysis. The titers of retroviruses were 2. 0 x 10(5) and 1.8 x 10(5), each for recombinant message and antisense hGRbeta cDNA.


CONCLUSIONS
We demonstrated here the obtaining of transfect PA317 cells that produce recombinant retroviruses containing message and antisense RNAs of hGRbeta with highly infective efficiencies.
Zhu
Min
M
Molecular Biology Research Center, Xiangya School of Medicine, Central South University, Changsha 410078, China.
He
Qing-nan
QN
Zhou
Gang
G
Zhang
Lei
L
Msangi
C I
CI
Hu
Wei-xin
WX
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D016373:DNA, Antisense / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection
D016373:DNA, Antisense; D018076:DNA, Complementary; D011965:Receptors, Glucocorticoid; D011994:Recombinant Proteins; C121943:glucocorticoid receptor beta