title: [Effects of an antioxidant (An7845) on the proliferation and apoptosis of K562 leukemia cells].
abstract: OBJECTIVE
  To determine the effects of an antioxidant (An7845) on the proliferation inhibition and apoptosis induction of a human leukemia cell line (K562).
  
  
  METHODS
  We evaluated the effects of An7845 on K562 cell proliferation by cellular enumeration in liquid culture assay, MTT assay and colony formation assay. We also verified the effects of An7845 on K562 cell apoptosis by cellular morphology and the DNA fragments appeared in agarose gel electrophoresis.
  
  
  RESULTS
  The proliferation of K562 leukemia cells was inhibited by An7845 (5 x 10(-8) - 5 x 10(5) mol/L) in a dose-dependent manner. K562 cells were induced toward apoptosis by An7845 (5 x 10(-7) mol/L).
  
  
  CONCLUSIONS
  An7845 can effectively inhibit the proliferation and induce the apoptosis of K562 leukemia cells.
authors.affiliation: Research Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China. hqun888@tom.com
authors.forename: Kui
authors.initials: K
authors.lastname: Song
authors.forename: Qun
authors.initials: Q
authors.lastname: He
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020014:K562 Cells
chemical_list: D000975:Antioxidants