Rapid diagnosis of orbital mantle cell lymphoma utilizing fluorescent in situ hybridization technology.
OBJECTIVE
We describe a patient with an orbital lymphoma in which genetic analysis utilizing fluorescent in situ hybridization (FISH) on a touch preparation of the tumor identified the classic 11:14 translocation associated with a mantle cell lymphoma.


METHODS
Clinicopathologic case report.


METHODS
A 76-year-old woman presented complaining of ptosis and was found to have an orbital lesion suspected of being a lymphoproliferative malignancy. A biopsy of the lesion was performed in the office, and the sample was processed using a touch preparation technique.


RESULTS
Genetic analysis utilizing FISH on a touch preparation of the tumor identified the classic 11:14 translocation associated with a mantle cell lymphoma.


CONCLUSIONS
FISH has become useful in the differential diagnosis of lymphoproliferative lesions. The touch preparation method requires smaller amounts of tissue than standard methods, and samples may be obtained in an office setting.
Coffee
Robert
R
Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, One Baylor Plaza, MS 220, Houston, TX 77030, USA. rcoffee@bcm.tmc.edu
Lazarchick
John
J
Chévez-Barrios
Patricia
P
Howard
Gene
G
D000368:Aged; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis* / Q000235:genetics; D009918:Orbital Neoplasms / Q000175:diagnosis* / Q000235:genetics; D014178:Translocation, Genetic / Q000235:genetics