title: Efficacy of directed misting application of a peroxygen disinfectant for environmental decontamination of a veterinary hospital.
abstract: OBJECTIVE
  To evaluate effectiveness of 4% peroxymonosulfate disinfectant applied as a mist to surfaces in a large animal hospital as measured by recovery of Staphylococcus aureus and Salmonella enterica serovar Typhimurium.
  
  
  METHODS
  Field trial.
  
  
  METHODS
  Polyester transparencies inoculated with bacteria.
  
  
  METHODS
  Polyester transparencies were inoculated with S aureus or S Typhimurium and placed in various locations in the hospital. After mist application of the peroxygen disinfectant, viable bacterial numbers were quantified and compared with growth from control transparencies to assess reduction in bacterial count.
  
  
  RESULTS
  When applied as a mist directed at environmental surfaces contaminated with a geometric mean of 4.03 x 10(7) CFUs of S aureus (95% confidence interval [CI], 3.95 x 10(7) to 4.11 x 10(7)) or 6.17 x 10(6) CFUs of S Typhimurium (95% Cl, 5.55 x 10(6) to 6.86 x 10(6)), 4% peroxymonosulfate reduced the geometric mean number of viable S aureus by 3.04 x 10(7) CFUs (95% Cl, 8.6 x 10(5) to 1.7 x 10(6)) and S Typhimurium by 3.97 x 10(6) CFUs (95% Cl, 8.6 x 10(5) to 3.5 x 10(6)).
  
  
  CONCLUSIONS
  Environmental disinfection with directed mist application of a 4% peroxymonosulfate solution was successful in reducing counts of bacterial CFUs by > 99.9999%. Directed mist application with this peroxygen disinfectant as evaluated in this study appeared to be an effective and efficient means of environmental disinfection in a large animal veterinary hospital and would be less disruptive than more traditional approaches to intensive environmental cleaning and disinfection.
authors.affiliation: Animal Population Health Institute, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Gage
authors.initials: G
authors.lastname: Patterson
authors.forename: Paul S
authors.initials: PS
authors.lastname: Morley
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Blehm
authors.forename: David E
authors.initials: DE
authors.lastname: Lee
authors.forename: Magda
authors.initials: M
authors.lastname: Dunowska
mesh_terms: D000336:Aerosols; D000818:Animals; D015169:Colony Count, Microbial / Q000662:veterinary; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D004783:Environmental Microbiology; D006762:Hospitals, Animal / Q000592:standards*; D010545:Peroxides / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000336:Aerosols; D004202:Disinfectants; D010545:Peroxides; C038288:peroxymonosulfate