Evolution of Buchloë dactyloides based on cloning and sequencing of matK, rbcL, and cob genes from plastid and mitochondrial genomes.
Buffalograss (Buchloë dactyloides (Nutt.) Englem), a C4 turfgrass species, is native to the Great Plains region of North America. The evolutionary implications of buffalograss are unclear. Sequencing of rbcL and matK genes from plastid and the cob gene from mitochondrial genomes was examined to elucidate buffalo grass evolution. This study is the first to report sequencing of these genes from organelle genomes in the genus Buchloë. Comparisons of sequence data from the mitochondrial and plastid genome revealed that all genotypes contained the same cytoplasmic origin. There were some rearrangements detected in mitochondrial genome. The buffalograss genome appears to have evolved through the rearrangements of convergent subgenomic domains. Combined analyses of plastid genes suggest that the evolutionary process in Buchloë accessions studied was monophyletic rather than polyphyletic. However, since plastid and mitochondrial genomes are generally uniparentally inherited, the evolutionary history of these genomes may not reflect the evolutionary history of the organism, especially in a species in which out-crossing is common. The sequence information obtained from this study can be used as a genome-specific marker for investigation of the buffalograss polyploidy complex and testing of the mode of plastid and mitochondrial transmission in genus Buchloë.
Budak
Hikmet
H
Sabanci University, Faculty of Engineering and Natural Science, Biological Science, and Bioengineering, Istanbul, Turkey. budak@sabanciuniv.edu
Shearman
Robert C
RC
Dweikat
Ismail
I
D002736:Chloroplasts / Q000201:enzymology* / Q000235:genetics; D045303:Cytochromes b / Q000235:genetics*; D004722:Endoribonucleases / Q000235:genetics*; D019143:Evolution, Molecular; D024101:Mitochondrial Proteins / Q000235:genetics; D009713:Nucleotidyltransferases / Q000235:genetics*; D010802:Phylogeny; D006109:Poaceae / Q000235:genetics*; D011123:Polyploidy; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*
D024101:Mitochondrial Proteins; D045303:Cytochromes b; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase