[Non-apoptotic, non-necrotic death of neutrophils induced by EP3 agonist--ONO-AE-248].
OBJECTIVE
To investigate the effect of EP(3) agonist--ONO-AE-248 on neutrophils.


METHODS
Electron microscopy, MTS assay, RT-PCR and FACS were used to observe the morphology, viability, EP(3) receptor expression and apoptosis of neutrophils treated with ONO-AE-248.


RESULTS
EP(3) receptor was expressed on neutrophils. ONO-AE-248 rapidly caused a unique form of neutrophil death, showing morphological changes of nucleus, including fusion of the lobules, blebbing and rupture of the nuclear membrane, which were different from typical morphological changes of apoptosis and necrosis. The death was dependent on ATP.


CONCLUSIONS
The death of neutrophils caused by ONO-AE-248 is likely to be one of the forms of "non-apoptotic programmed cell death".
Liu
Jia-jia
JJ
Department of Molecular Biology, Luzhou Medical College, Luzhou 646000, China. jiajialiu08@163.ccom
Zhang
Zi
Z
He
Hao
H
Li
Xin
X
He
Yan-zheng
YZ
D017209:Apoptosis; D016923:Cell Death / Q000187:drug effects*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000031:analogs & derivatives / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D046529:Microscopy, Electron, Transmission; D009336:Necrosis; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects*; D018078:Receptors, Prostaglandin E / Q000819:agonists*; D058308:Receptors, Prostaglandin E, EP3 Subtype; D020133:Reverse Transcriptase Polymerase Chain Reaction
C121546:ONO AE 248; C545647:PTGER3 protein, human; D018078:Receptors, Prostaglandin E; D058308:Receptors, Prostaglandin E, EP3 Subtype; D015232:Dinoprostone