Optimization of culture conditions and medium composition for the production of micrococcin GO5 by Micrococcus sp. GO5.
To enhance the production of micrococcin GO5, a bacteriocin produced by Micrococcus sp. GO5, cultivation conditions and medium composition were optimized. The optimal initial pH and temperature for bacteriocin production were 7.0-9.0 and 37 degrees C, respectively. Micrococcus sp. GO5 displayed the highest micrococcin GO5 activity when grown in modified MRS medium that contained lactose or sucrose, rather than glucose, as a carbon source. The maximum bacteriocin activity was obtained in modified MRS medium containing 0.5% tryptone and 1.0% yeast extract as nitrogen sources instead of the other nitrogen sources present in MRS medium. Bacteriocin production was greatly affected by the concentration of K(2)HPO(4); strain GO5 produced eight-fold more bacteriocin in medium containing 2.0-2.5% K(2)HPO(4) than in medium containing 0.2% K(2)HPO(4). The optimal concentration of MgSO(4).7H(2)O for bacteriocin production was 0.5%. The production of micrococcin GO5 was increased 32-fold in shake flask culture and 16-fold in a bioreactor using the optimized medium (TY medium), compared with culturing in MRS medium.
Kim
Mi-Hee
MH
Department of Biological Science and Biotechnology, Hankuk University of Foreign Studies, Yongin 120-749, Republic of Korea.
Kong
Yoon-Jung
YJ
Baek
Hong
H
Hyun
Hyung-Hwan
HH
D001430:Bacteriocins / Q000096:biosynthesis*; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008278:Magnesium Sulfate / Q000737:chemistry; D008837:Micrococcus / Q000254:growth & development*; D010455:Peptides; D010710:Phosphates / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry
D001430:Bacteriocins; D003470:Culture Media; D010455:Peptides; D010710:Phosphates; D017680:Potassium Compounds; C009779:micrococcin; D008278:Magnesium Sulfate; C013216:potassium phosphate