Analysis of mitochondrial DNA variability and genetic structure in populations of New World screwworm flies (Diptera: Calliphoridae) from Uruguay.
The New Word screwworm, Cochliomyia hominivorax (Coquerel 1858) (Diptera: Calliphoridae), is one of the most important insect pests of livestock in the Neotropical region. In this work, polymerase chain reaction-restriction fragment length polymorphism of mitochondrial DNA (mtDNA) was used to study the diversity and population structure of seven geographically distinct populations of C. hominivorax from most of the important livestock areas in Uruguay. The control region (A+T/12S) and subunits 1 and 2 of cytochrome oxidase (cox1/cox2) were amplified and digested with restriction endonucleases. Nine haplotypes were observed among the populations sampled. The mean nucleotide diversity and the haplotype diversity indicated high mtDNA variability in this species. The similarity index, average nucleotide divergence, and analysis of molecular variance results showed no evidence of subpopulation differentiation, indicating that the C. hominivorax populations of Uruguay form a single panmitic population. The distribution pattern of the genetic variation in natural populations of C. hominivorax and the implications of these results for establishing control program are discussed.
Lyra
Mariana Lúcio
ML
Laboratório de Genética Animal, Centro de Biologia Molecular e Engenharia Genética, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), P.O. Box 6010, Campinas, São Paulo, 13083-875, Brazil. marilyra@unicamp.br
Fresia
Pablo
P
Gama
Santos
S
Cristina
Juan
J
Klaczko
Louis Bernard
LB
Lima De Azeredo-Espin
Ana Maria
AM
D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D004175:Diptera / Q000235:genetics*; D014644:Genetic Variation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014583:Uruguay
D004272:DNA, Mitochondrial