title: Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map.
abstract: The concept of selective (or bin) mapping is used here for the first time, using as an example the Prunus reference map constructed with an almond x peach F2 population. On the basis of this map, a set of six plants that jointly defined 65 possible different genotypes for the codominant markers mapped on it was selected. Sixty-three of these joint genotypes corresponded to a single chromosomal region (a bin) of the Prunus genome, and the two remaining corresponded to two bins each. The 67 bins defined by these six plants had a 7.8-cM average length and a maximum individual length of 24.7 cM. Using a unit of analysis composed of these six plants, their F1 hybrid parent, and one of the parents of the hybrid, we mapped 264 microsatellite (or simple-sequence repeat, SSR) markers from 401 different microsatellite primer pairs. Bin mapping proved to be a fast and economic strategy that could be used for further map saturation, the addition of valuable markers (such as those based on microsatellites or ESTs), and giving a wider scope to, and a more efficient use of, reference mapping populations.
authors.affiliation: Departament de Genètica Vegetal, Laboratori de Genètica Molecular Vegetal, CSIC-IRTA, 08348 Cabrils (Barcelona), Spain.
authors.forename: Werner
authors.initials: W
authors.lastname: Howad
authors.forename: Toshiya
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Dirlewanger
authors.forename: Raffaele
authors.initials: R
authors.lastname: Testolin
authors.forename: Patrick
authors.initials: P
authors.lastname: Cosson
authors.forename: Guido
authors.initials: G
authors.lastname: Cipriani
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Monforte
authors.forename: Laura
authors.initials: L
authors.lastname: Georgi
authors.forename: Albert G
authors.initials: AG
authors.lastname: Abbott
authors.forename: Pere
authors.initials: P
authors.lastname: Arús
mesh_terms: D002874:Chromosome Mapping; D005799:Genes, Dominant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D018598:Minisatellite Repeats; D027861:Prunus / Q000235:genetics*
chemical_list: D005819:Genetic Markers