Post-mortem human brain autoradiography of the norepinephrine transporter using (S,S)-[18F]FMeNER-D2.
The binding of the norepinephrine transporter radioligand, (S,S)-[18F]FMeNER-D2, to human brain post-mortem was examined in vitro by whole hemisphere autoradiography. The rank order for the density of labelling was: locus coeruleus>>cortex approximately cerebellum approximately thalamus>caudate approximately putamen. The NET-selectivity of binding was confirmed by co-incubation with desipramine. The dual NET/SERT inhibitor duloxetine also inhibited specific binding, whereas PE2I or citalopram had no evident effect.
Schou
Magnus
M
Karolinska Institutet, Department of Clinical Neuroscience, Psychiatry Section, Karolinska Hospital, S-17176 Stockholm, Sweden. magnus.schou@cns.ki.se
Halldin
Christer
C
Pike
Victor W
VW
Mozley
P David
PD
Dobson
David
D
Innis
Robert B
RB
Farde
Lars
L
Hall
Håkan
H
D018759:Adrenergic Uptake Inhibitors / Q000494:pharmacology; D001344:Autopsy; D001345:Autoradiography; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D003891:Desipramine / Q000494:pharmacology; D000068736:Duloxetine Hydrochloride; D005462:Fluorine Radioisotopes / Q000493:pharmacokinetics; D006801:Humans; D009025:Morpholines / Q000493:pharmacokinetics*; D011180:Postmortem Changes; D011869:Radioligand Assay; D013876:Thiophenes / Q000494:pharmacology
C506462:2-(alpha-(2-fluoromethoxyphenoxy)benzyl)morpholine; D018759:Adrenergic Uptake Inhibitors; D005462:Fluorine Radioisotopes; D009025:Morpholines; D013876:Thiophenes; D000068736:Duloxetine Hydrochloride; D003891:Desipramine