title: Mutations associated with sulfadoxine-pyrimethamine and chlorproguanil resistance in Plasmodium falciparum isolates from Blantyre, Malawi.
abstract: We conducted a prevalence study of mutations in Plasmodium falciparum that are associated with antifolate resistance in Blantyre, Malawi. The dihydrofolate reductase 164-Leu mutation, which confers resistance to both pyrimethamine and chlorproguanil, was found in 4.7% of the samples. Previously unreported mutations in dihydropteroate synthase were also found.
authors.affiliation: Department of Epidemiology, University of North Carolina School of Public Health, Chapel Hill, NC 27599-7435, USA. alker@unc.edu
authors.forename: Alisa P
authors.initials: AP
authors.lastname: Alker
authors.forename: Victor
authors.initials: V
authors.lastname: Mwapasa
authors.forename: Anne
authors.initials: A
authors.lastname: Purfield
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Rogerson
authors.forename: Malcolm E
authors.initials: ME
authors.lastname: Molyneux
authors.forename: Deborah D
authors.initials: DD
authors.lastname: Kamwendo
authors.forename: Eyob
authors.initials: E
authors.lastname: Tadesse
authors.forename: Ebbie
authors.initials: E
authors.lastname: Chaluluka
authors.forename: Steven R
authors.initials: SR
authors.lastname: Meshnick
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D017931:DNA Primers; D004338:Drug Combinations; D004351:Drug Resistance; D005493:Folic Acid Antagonists / Q000494:pharmacology; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology; D008295:Malawi; D009154:Mutation; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000235:genetics*; D002727:Proguanil / Q000031:analogs & derivatives* / Q000494:pharmacology; D011739:Pyrimethamine / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013413:Sulfadoxine / Q000494:pharmacology*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics / Q000378:metabolism
chemical_list: D000962:Antimalarials; D017931:DNA Primers; D004338:Drug Combinations; D005493:Folic Acid Antagonists; D013413:Sulfadoxine; C007030:chlorproguanil; D013762:Tetrahydrofolate Dehydrogenase; D002727:Proguanil; D011739:Pyrimethamine