[Analysis of succeed percentage on sequencing ESTs and construction of porcine muscle cDNA library].
Using Longissimus Dorsi muscle as material and Lambda ZAP II as Vector, Xiang Pig Longissimus Dorsi muscle cDNA library has been constructed in our study. The results showed that the titration of the library was 3.4 x 10(7) pfu/ml, the recombinant percentage was 94%, and the fragment length of inserted average cDNA were 1.5 kb. The study pointed out that the more than 30 T insertion is the major factor for low percentage if sequencing the 3'-end.
Wang
Xiu-Li
XL
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China.
Li
Ning
N
Zhao
Zhi-Hui
ZH
Feng
Ji-Dong
JD
Zhao
Xing-Bo
XB
Li
Chang-Lv
CL
Wu
Chang-Xin
CX