The tropical cedar tree (Cedrela fissilis Vell., Meliaceae) homolog of the Arabidopsis LEAFY gene is expressed in reproductive tissues and can complement Arabidopsis leafy mutants.
A homolog of FLORICAULA/LEAFY, CfLFY (for Cedrela fissilis LFY), was isolated from tropical cedar. The main stages of the reproductive development in C. fissilis were documented by scanning electron microscopy and the expression patterns of CfLFY were studied during the differentiation of the floral meristems. Furthermore, the biological role of the CfLFY gene was assessed using transgenic Arabidopsis plants. CfLFY showed a high degree of similarity to other plant homologs of FLO/LFY. Southern analysis showed that CfLFY is a single-copy gene in the tropical cedar genome. Northern blot analysis and in situ hybridization results showed that CfLFY was expressed in the reproductive buds during the transition from vegetative to reproductive growth, as well as in floral meristems and floral organs but was excluded from the vegetative apex and leaves. Transgenic Arabidopsis lfy26 mutant lines expressing the CfLFY coding region, under the control of the LFY promoter, showed restored wild-type phenotype. Taken together, our results suggest that CfLFY is a FLO/LFY homolog probably involved in the control of tropical cedar reproductive development.
Dornelas
Marcelo Carnier
MC
Centro de Energia Nuclear na Agricultura. Laboratório de Biotecnologia Vegetal, Universidade de São Paulo, Av. Centenário, 303 CEP, 13400-970 Piracicaba, SP, Brazil. mcdornel@cena.usp.br
Rodriguez
Adriana Pinheiro Martinelli
AP
D017360:Arabidopsis / Q000235:genetics / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics; D031587:Cedrela / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D003001:Cloning, Molecular; D035264:Flowers / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D005816:Genetic Complementation Test; D017403:In Situ Hybridization; D018519:Meristem / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009154:Mutation; D016366:Open Reading Frames; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism / Q000648:ultrastructure; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; C074747:LFY protein, Arabidopsis; D010940:Plant Proteins; D014157:Transcription Factors