Noninvasive intraocular pressure measurements in mice by pneumotonometry.
OBJECTIVE
To develop a reliable, noninvasive, continuous, and easily implemented system for measuring intraocular pressure (IOP) in mice.


METHODS
Pneumotonometry was adapted for measurement of mouse IOP. Measurements were compared with those obtained with the servo-null micropipette system (SNMS) and with direct anterior chamber cannulation. Heart rate was monitored by the precordial pulse, EKG, or tail pulse in anesthetized mice. The characteristic ocular hypotensive response to mannitol was assessed as an additional validation of the


METHODS



RESULTS
Measurements of IOP obtained using pneumotonometry agreed closely with values measured by SNMS or by direct cannulation. IOP oscillations were synchronous with the heart rate, with a coherence peak between them of approximately 2 Hz, equal to the pulse frequency. Hypertonic mannitol reduced IOP from 13.7 +/- 0.9 mm Hg by 7.7 +/- 0.7 mm Hg after 15 minutes.


CONCLUSIONS
Pneumotonometry is a reliable and noninvasive method for the measurement of IOP in mice and may permit comparisons of IOP to hemodynamic factors. This system is simpler and more adaptable for glaucoma research than previously reported methodologies for measuring IOP in mice.
Avila
Marcel Y
MY
Department of Physiological Sciences, Facultad de Medicina, Universidad Nacional de Colombia, Bogota, Colombia.
Múnera
Alejandro
A
Guzmán
Arcadio
A
Do
Chi Wai
CW
Wang
Zhao
Z
Stone
Richard A
RA
Civan
Mortimer M
MM
D000818:Animals; D006339:Heart Rate; D007429:Intraocular Pressure / Q000187:drug effects / Q000502:physiology*; D008353:Mannitol / Q000008:administration & dosage; D051379:Mice; D008810:Mice, Inbred C57BL; D015814:Ocular Hypotension / Q000139:chemically induced / Q000503:physiopathology; D015203:Reproducibility of Results; D014065:Tonometry, Ocular / Q000379:methods*
D008353:Mannitol