title: Morphological variations of Anopheles minimus A in Tak Province, Thailand.
abstract: Anopheles minimus Theobald is one of the major vectors of malaria throughout the Oriental Region, and it's complex consists of at least 2 sibling species (A and C) in Thailand. This study aimed to determine the morphological variations of wings of An. minimus A and to clarify the specific status of An. minimus in Ban Khun Huay, Ban Pa Dae, and Ban Tham Seau, Mae Sot district, Tak Province, Thailand. Anopheline larvae were collected from the fields between October 2002 and September 2003, allowed to emerge into adults in the laboratory and identified by morphological and molecular characterization. About 1,715 of female An. minimus A were separated into 8 groups based on their wing scale patterns. Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) assay (ribosomal DNA ITS2) confirmed the identification of An. minimus A in all 8 groups.
authors.affiliation: Department of Entomology, US Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
authors.forename: Boonsong
authors.initials: B
authors.lastname: Jaichapor
authors.forename: Ampornpan
authors.initials: A
authors.lastname: Kengluecha
authors.forename: Pornpimol
authors.initials: P
authors.lastname: Rongnoparut
authors.forename: Leopoldo M
authors.initials: LM
authors.lastname: Rueda
authors.forename: James W
authors.initials: JW
authors.lastname: Jones
authors.forename: Ratana
authors.initials: R
authors.lastname: Sithiprasasna
mesh_terms: D000818:Animals; D000852:Anopheles / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics; D017931:DNA Primers; D005260:Female; D007303:Insect Vectors / Q000033:anatomy & histology* / Q000235:genetics; D007814:Larva; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D013785:Thailand; D014921:Wings, Animal / Q000033:anatomy & histology
chemical_list: D017931:DNA Primers