title: Immunogenicity and efficacy of three recombinant subunit Pasteurella multocida toxin vaccines against progressive atrophic rhinitis in pigs.
abstract: Three short fragments of recombinant subunit Pasteurella multocida toxin (rsPMT) were constructed for evaluation as candidate vaccines against progressive atrophic rhinitis (PAR) of swine. PMT-specific antibody secreting cells and evidence of cellular immunity were detected in rsPMT-immunized pigs following authentic PMT challenge or homologous antigen booster. Piglets immunized with rsPMT fragments containing either the N-terminal or the C-terminal portions of PMT developed high titers of neutralizing antibodies. Pregnant sows immunized with rsPMT had higher levels of maternal antibodies in their colostrum than did those immunized with a conventional PAR-toxoid vaccine. Offspring from rsPMT vaccinated sows had better survival after challenge with a five-fold lethal dose of authentic PMT and had better growth performance after challenge with a sublethal dose of toxin. Our findings indicate these non-toxic rsPMT proteins are attractive candidates for development of a subunit vaccine against PAR in pigs.
authors.affiliation: Graduate Institute of Veterinary Pathology, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan, ROC.
authors.forename: Chih-Ming
authors.initials: CM
authors.lastname: Liao
authors.forename: Chienjin
authors.initials: C
authors.lastname: Huang
authors.forename: Shih-Ling
authors.initials: SL
authors.lastname: Hsuan
authors.forename: Zeng-Weng
authors.initials: ZW
authors.lastname: Chen
authors.forename: Wei-Cheng
authors.initials: WC
authors.lastname: Lee
authors.forename: Cheng-I
authors.initials: CI
authors.lastname: Liu
authors.forename: James R
authors.initials: JR
authors.lastname: Winton
authors.forename: Maw-Sheng
authors.initials: MS
authors.lastname: Chien
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D007114:Immunization; D008213:Lymphocyte Activation; D016979:Pasteurella multocida / Q000276:immunology*; D012222:Rhinitis, Atrophic / Q000517:prevention & control / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D022223:Vaccines, Subunit / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; C062258:Pasteurella multocida toxin; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic