title: Cytochrome c dissociation and release from mitochondria by truncated Bid and ceramide.
abstract: We have examined the effects of truncated Bid (tBid) and ceramide on mitochondrial membrane integrity and cytochrome c release, using mitochondria with intact outer membranes. While tBid permeabilizes the outer membrane and efficiently stimulates cytochrome c release, digitonin is unable to cause cytochrome c release in the absence of salt. Ceramides did not permeabilize the mitochondrial outer membrane, and stimulated cytochrome c release only in the presence of digitonin. Taken together, these observations support a model for cytochrome c release in which the first step is dissociation from the inner membrane followed by transit across the outer membrane.
authors.affiliation: Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Yuan
authors.forename: Scott D
authors.initials: SD
authors.lastname: Williams
authors.forename: Souichi
authors.initials: S
authors.lastname: Adachi
authors.forename: Tilman
authors.initials: T
authors.lastname: Oltersdorf
authors.forename: Roberta A
authors.initials: RA
authors.lastname: Gottlieb