Single-walled carbon nanotube biosensors using aptamers as molecular recognition elements.
We report the real-time detection of protein using SWNT-FET-based biosensors comprising DNA aptamers as molecular recognition elements. Anti-thrombin aptamers that are highly specific to serine protein thrombin were immobilized on the sidewall of a SWNT-FET using CDI-Tween linking molecules. The binding of thrombin aptamers to SWNT-FETs causes a rightward shift of the threshold gate voltages, presumably due to the negatively charged backbone of the DNA aptamers. While the addition of thrombin solution causes an abrupt decrease in the conductance of the thrombin aptamer immobilized SWNT-FET, no noticeable change was observed with elastase.
So
Hye-Mi
HM
Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 305-343, Korea.
Won
Keehoon
K
Kim
Yong Hwan
YH
Kim
Byoung-Kye
BK
Ryu
Beyong Hwan
BH
Na
Pil Sun
PS
Kim
Hyojin
H
Lee
Jeong-O
JO
D001483:Base Sequence; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013917:Thrombin / Q000737:chemistry*; D013997:Time Factors
D037742:Nanotubes, Carbon; D009841:Oligonucleotides; D004247:DNA; D013917:Thrombin