title: [Correlative study on the expression of ER mRNA and presenilin 2 in the pancreatic carcinoma tissues].
abstract: OBJECTIVE
  To study the expression of ER mRNA, presenilin 2 (PS2) and their relationship and clinicopathological significance in the pancreatic carcinoma.
  
  
  METHODS
  The assay of ER mRNA was detected by in situ hybridization technique and the expression of PS2 was completed by immunohistochemical method of avidin-biotin complex in the 10% neutral formalin-fixed and routinely paraffin-embedded sections of specimen with pancreatic carcinoma.
  
  
  RESULTS
  The positive rate and the score of PS2 were 45.7% and 1.82 +/- 1.34, also those of ER mRNA were 51.4% and 2.08 +/- 1.46 in 35 patients with pancreatic carcinoma. The highly positive correlations were found among the positive rate and the score of ER mRNA and PS2. The positive rate and the score were significantly higher in the cases of well-differentiated adenocarcinoma without metastasis than those in the ones of middle- or poorly-differentiated adenocarcinoma with metastasis.
  
  
  CONCLUSIONS
  The expression of ER mRNA or PS2 might reflect the biological behaviors and hormone-dependent status of pancreatic carcinoma. The assay of ER mRNA and meanwhile the detection of PS2 might have important clinical values for better understanding sex-hormone dependence and raising the therapeutic effect of clinical endocrine therapy of the pancreatic carcinoma.
authors.affiliation: Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Sheng-Fu
authors.initials: SF
authors.lastname: Huang
authors.forename: Zhu-Lin
authors.initials: ZL
authors.lastname: Yang
authors.forename: Xing-Hui
authors.initials: XH
authors.lastname: Deng
authors.forename: Xiong-Ying
authors.initials: XY
authors.lastname: Miao
authors.forename: Zan-Geng
authors.initials: ZG
authors.lastname: Zhuang
authors.forename: Yong-Guo
authors.initials: YG
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D053766:Presenilin-2; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D008565:Membrane Proteins; C508861:PSEN2 protein, human; D053766:Presenilin-2; D012333:RNA, Messenger; D011960:Receptors, Estrogen