Flow-injection with enhanced chemiluminescence detection of ofloxacin in human plasma.
A simple, rapid and sensitive method for the determination of ofloxacin in plasma has been developed based on flow-injection analysis with enhanced chemiluminescence (CL) detection. This method employs the CL reaction of cerium(IV) and sulphite sensitized by a Tb(III)-ofloxacin complex. Plasma samples were deproteinized with acetonitrile before analysis. Under optimal conditions, the method allowed the quantitative analysis of ofloxacin in plasma over the range of 22.5-900 ng/mL with a detection limit of 7.5 ng/mL (3sigma). The intra-day and inter-day precision was < 3.0% (n = 11) and < 7.0% (n = 3), respectively. The method was a convenient tool, rapid (20 s/analysis) and well suited for pharmacokinetics studies in which thousands of samples must be analysed daily. It was applied to pharmacokinetics studies of ofloxacin.
Zhang
Xianhua
X
Department of Pharmacy, Peking University Third Hospital, Peking University TDMCT Centre, Beijing 100083, People's Republic of China.
Ouyang
Jin
J
Zhai
Suodi
S
Huang
Guangming
G
Zhao
Huichun
H
D000143:Acids / Q000737:chemistry; D002138:Calibration; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D015394:Molecular Structure; D015242:Ofloxacin / Q000097:blood* / Q000737:chemistry; D016877:Oxidants / Q000737:chemistry; D011522:Protons; D019163:Reducing Agents / Q000737:chemistry; D012680:Sensitivity and Specificity; D013725:Terbium
D000143:Acids; D016877:Oxidants; D011522:Protons; D019163:Reducing Agents; D013725:Terbium; D015242:Ofloxacin