[Identification of expanding-related genes from young fruit of cucumber after pollination].
Differential expression of genes in young fruit of cucumber (Cucumis sativus L.) before/after pollination was investigated via cDNA amplified fragment length polymorphism (cDNA-AFLP). There were 64 transcript-derived fragments (TDFs) displayed specially in young fruits after pollination of 6-72 h (Table 1), five of which were verified to be mainly expressed in young fruits by reverse Northern dot blotting (Fig.2). Sequence data showed one TDF was a new gene encoding expansin and was named as Cs-Expansin10 (CsExp10), which might be related to the expanding growth of cucumber fruit after pollination (Fig.3), another was significantly similar to the glutathione reductase gene, and the others had no similar sequence in GenBank database (Table 2).
Sun
Yong-Dong
YD
Key Lab in Vegetable Research of Chongqing, College of Horticulture and Landscape, Southwest Agricultural University, Chongqing 400716, China.
Zhang
Xing-Guo
XG
Hou
Rui-Xian
RX
Guo
Su-Ying
SY
Hao
Feng
F
D000595:Amino Acid Sequence; D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D015152:Blotting, Northern; D018553:Cucumis sativus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D054817:Pollination / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment
D010940:Plant Proteins