title: Over-representation of bcr3 subtype of PML/RARalpha fusion gene in APL in Indian patients.
abstract: Thirty six patients with acute promyelocytic leukemia were studied by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR. There was concordance between the results achieved by both the methods except in one case, which was negative by RT-PCR but positive by real-time PCR. The prevalence of bcr3 (short isoform) was found to be significantly higher than that of bcr1 (long isoform) (64 vs. 36%, P=0.03). No correlation was found between age, sex, and white blood cell (WBC) count at diagnosis. Molecular remission was achieved in 66.6% of patients with bcr3 isoform. Median WBC count at presentation was found to be higher than that in the West.
authors.affiliation: Department of Haematology, All India Institute of Medical Sciences, New Delhi, 110 029, India.
authors.forename: Sudha
authors.initials: S
authors.lastname: Sazawal
authors.forename: Syed Khizer
authors.initials: SK
authors.lastname: Hasan
authors.forename: Pankhi
authors.initials: P
authors.lastname: Dutta
authors.forename: Bijender
authors.initials: B
authors.lastname: Kumar
authors.forename: Rajat
authors.initials: R
authors.lastname: Kumar
authors.forename: Lalit
authors.initials: L
authors.lastname: Kumar
authors.forename: V P
authors.initials: VP
authors.lastname: Choudhry
authors.forename: Renu
authors.initials: R
authors.lastname: Saxena
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007194:India; D015473:Leukemia, Promyelocytic, Acute / Q000097:blood* / Q000175:diagnosis; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D015514:Oncogene Proteins, Fusion / Q000096:biosynthesis*; D015995:Prevalence; D020033:Protein Isoforms / Q000096:biosynthesis; D051562:Proto-Oncogene Proteins c-bcr / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D020033:Protein Isoforms; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein; D051562:Proto-Oncogene Proteins c-bcr