title: Relationship between IL-1beta gene polymorphism and gastric mucosal IL-1beta levels in patients with Helicobacter pylori infection.
abstract: BACKGROUND
  Interkeukin-1 (IL-1) gene cluster polymorphisms that are thought to enhance the production of IL-1beta are associated with an increased risk of gastric cancer. To determine the role of host genetic factors in Helicobacter pylori infection, we examined the relationship between gastric mucosal IL-1beta levels and IL-1B polymorphisms in patients with H. pylori infection.
  
  
  METHODS
  Biopsy tissues obtained from 99 patients were homogenized and gastric mucosal IL-1beta levels were measured by enzyme-linked immunosorbent assay (ELISA). Single-base polymorphisms at positions -511 and -31 in IL-1B were analyzed.
  
  
  RESULTS
  The IL-1beta level in the antrum was significantly higher in genotype IL-1B-511C/C than in H. pylori-negative patients (P < 0.05). The IL-1B polymorphism did not influence the degree of gastric neutrophil and mononuclear cell infiltration, or gastric atrophy. IL-1beta levels in the corpus, but not those in the antrum, correlated to the severity of gastric atrophy.
  
  
  CONCLUSIONS
  These findings indicate that IL-1B polymorphisms enhance IL-1beta production in the antrum; however, other factors might regulate the production of IL-1beta in the corpus of the stomach, regardless of IL-1B polymorphisms, and high IL-1beta production may be associated with the grade of gastric atrophy in the corpus mucosa in patients with H. pylori infection.
authors.affiliation: Department of Internal Medicine, Tokai University School of Medicine, Boseidai, Isehara, 259-1193, Japan.
authors.forename: Jiang
authors.initials: J
authors.lastname: Xuan
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Deguchi
authors.forename: Sumio
authors.initials: S
authors.lastname: Watanabe
authors.forename: Hideki
authors.initials: H
authors.lastname: Ozawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Urano
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Ryuki
authors.initials: R
authors.lastname: Fukuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kijima
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Koga
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takagi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001284:Atrophy; D001706:Biopsy; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry*; D016481:Helicobacter Infections / Q000235:genetics* / Q000378:metabolism*; D016480:Helicobacter pylori; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011706:Pyloric Antrum / Q000473:pathology
chemical_list: D007375:Interleukin-1