NMR spectroscopic characterization of the membrane affinity of polyols.
Residual dipolar couplings (RDCs) are applied here for the analysis of weak, transient binding events between phosphatidylcholine bilayers and polyols. Large signal responses are observed even for low percentages of 'ligand-receptor complexes', making RDCs a sensitive tool for the analysis of molecular recognition events. The different degree of alignment in solution can be compared as a result of the calculation of the alignment tensor elements. By varying polarity and/or charge of the molecules under investigation, nonspecific hydrophobic effects can be excluded.
Fischer
Daniela
D
Fachbereich Chemie, Philipps-Universität Marburg, D-35032 Marburg, Germany.
Geyer
Armin
A
D002247:Carbon Isotopes; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards; D008560:Membrane Fluidity; D015394:Molecular Structure; D010767:Phosphorylcholine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011522:Protons; D012015:Reference Standards
D002247:Carbon Isotopes; D008024:Ligands; D008051:Lipid Bilayers; D011108:Polymers; D011522:Protons; C024617:polyol; D010767:Phosphorylcholine