title: Formation of polyelectrolyte multilayer architectures with embedded DMPC studied in situ by neutron reflectometry.
abstract: The coupling of lipid molecules to polymer components in a planar biomimetic model membrane made of a lipid bilayer (dimyristoylphosphatidylcholine) supported by polyelectrolyte multilayers is studied. The polyelectrolyte support was prepared by layer-by-layer deposition of positively charged poly(allylamine hydrochloride) (PAH) and negatively charged poly(sodium 4-styrenesulfonate) (PSS). Two polymer sample terminations were considered: positively charged (PAH-terminated) and negatively charged (PSS-terminated). Neutron reflectometry studies showed that, whereas positively charged samples did not favor the deposition of lipid, negatively charged samples allowed the deposition of a lipid bilayer with a thickness of approximately 5 nm. In the latter case, formation of polyelectrolyte layers after the deposition of the lipid layer was also possible.
authors.affiliation: Max-Planck Institute of Colloids and Interfaces, Wissenschaftspark Golm, 14424 Potsdam, Germany.
authors.forename: Christophe
authors.initials: C
authors.lastname: Delajon
authors.forename: Thomas
authors.initials: T
authors.lastname: Gutberlet
authors.forename: Roland
authors.initials: R
authors.lastname: Steitz
authors.forename: Helmuth
authors.initials: H
authors.lastname: Möhwald
authors.forename: Rumen
authors.initials: R
authors.lastname: Krastev
mesh_terms: D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D015394:Molecular Structure; D009502:Neutrons
chemical_list: D004573:Electrolytes; D004134:Dimyristoylphosphatidylcholine