Identification of cold acclimated genes in leaves of Citrus unshiu by mRNA differential display.
Citrus unshiu is freeze tolerant to -10 degrees C when fully acclimated after exposure to cold, nonfreezing temperatures. To gain an understanding of its cold tolerance mechanism, mRNA differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) and quantitative relative RT-PCR were used to study gene expression under a gradual cold-acclimation temperature regime. Six up-regulated and two down regulated genes were identified based on their amino acid sequences. The identified proteins encoded by the up-regulated genes were: 14-3-3 protein, 40S ribosomal protein S23, putative 60S ribosomal protein L15, nucleoside diphosphate kinase III protein, regulator of chromosome condensation-like protein, and amino acid permease 6. The proteins encoded by the two down-regulated genes were: miraculin-like protein and beta-galactosidase. Their individual function has been briefly reviewed based on published information. In addition to the findings in this study, we compared the function of cold responsive genes of Poncirus trifoliata, a very cold hardy relative of Citrus species that is freeze tolerant to -30 degrees C when fully acclimated, to the function of genes in the current study.
Lang
Ping
P
Department of Horticulture, Auburn University, Auburn, AL, 36849 USA.
Zhang
Can-Kui
CK
Ebel
Robert C
RC
Dane
Fenny
F
Dozier
William A
WA
D048948:14-3-3 Proteins / Q000235:genetics; D000064:Acclimatization / Q000235:genetics*; D000591:Amino Acid Isomerases / Q000235:genetics; D002957:Citrus / Q000235:genetics*; D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D054778:NM23 Nucleoside Diphosphate Kinases; D009687:Nuclear Proteins / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D032104:Poncirus / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012269:Ribosomal Proteins / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
D048948:14-3-3 Proteins; D018076:DNA, Complementary; D054778:NM23 Nucleoside Diphosphate Kinases; D009687:Nuclear Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C043649:ribosomal protein L15; D009701:Nucleoside-Diphosphate Kinase; D001616:beta-Galactosidase; D000591:Amino Acid Isomerases