Poly(ADP-ribose) glycohydrolase is a component of the FMRP-associated messenger ribonucleoparticles.
PARG [poly(ADP-ribose) glycohydrolase] is the only known enzyme that catalyses the hydrolysis of poly(ADP-ribose), a branched polymer that is synthesized by the poly(ADP-ribose) polymerase family of enzymes. Poly(ADP-ribosyl)ation is a transient post-translational modification that alters the functions of the acceptor proteins. It has mostly been studied in the context of DNA-damage signalling or DNA transaction events, such as replication and transcription reactions. Growing evidence now suggests that poly(ADP-ribosyl)ation could have a much broader impact on cellular functions. To elucidate the roles that could be played by PARG, we performed a proteomic identification of PARG-interacting proteins by mass spectrometric analysis of PARG pulled-down proteins. In the present paper, we report that PARG is resident in FMRP (Fragile-X mental retardation protein)-associated messenger ribonucleoparticles complexes. The localization of PARG in these complexes, which are components of the translation machinery, was confirmed by sedimentation and microscopy analysis. A functional link between poly(ADP-ribosyl)ation modulation and FMRP-associated ribonucleoparticle complexes are discussed in a context of translational regulation.
Gagné
Jean-Philippe
JP
Health and Environment Unit, Laval University Medical Research Center, CHUQ, Faculty of Medicine, Laval University, 2705 Boulevard Laurier, Ste-Foy, Québec, Canada, G1V 4G2.
Bonicalzi
Marie-Eve
ME
Gagné
Pierre
P
Ouellet
Marie-Eve
ME
Hendzel
Michael J
MJ
Poirier
Guy G
GG
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002417:Cattle; D002522:Chlorocebus aethiops; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000378:metabolism*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000378:metabolism*
D051860:Fragile X Messenger Ribonucleoprotein 1; D006026:Glycoside Hydrolases; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; C022407:poly ADP-ribose glycohydrolase