title: Effect of some pharmaceutical excipients on the survival of probiotic vaginal lactobacilli.
abstract: Lactobacilli are the predominant microorganisms of the vaginal bacterial microbiota, and they play a major role in the maintenance of a healthy urogenital tract. In consequence, the interest in their potential use as probiotics has significantly increased during the last decade. In the present study we assessed the influence of different excipients on the survival of 4 probiotic vaginal lactobacilli incorporated into glycerinated gelatin ovules and stored at 5 degrees C for 60 d. Results showed that viability after storage was a strain-dependent characteristic, but inclusion of ascorbic acid significantly increased survival in 3 of the 4 strains tested. The best survival was observed for Lactobacillus salivarius CRL 1328 in ovules containing skimmed milk. No significant differences in viability were observed between control ovules (glycerogelatin base without excipients) and those containing lactose or Tween 80 for any of the strains tested. Lactobacillus acidophilus CRL 1259 and Lactobacillus crispatus CRL 1266 were, respectively, the most resistant and sensitive strains to the storage with the different substances. In conclusion, these results provide a basis for selecting excipients to improve the survival of lactobacilli in a probiotic product, in an attempt to ensure the delivery of an adequate number of viable cells to the urogenital tract.
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, San Miguel de Tucumán, Argentina.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Zárate
authors.forename: María Silvina Juárez
authors.initials: MS
authors.lastname: Tomás
authors.forename: María Elena
authors.initials: ME
authors.lastname: Nader-Macias
mesh_terms: D000818:Animals; D001205:Ascorbic Acid; D005079:Excipients / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects* / Q000254:growth & development; D008892:Milk; D019936:Probiotics; D013045:Species Specificity; D014621:Vagina / Q000382:microbiology
chemical_list: D005079:Excipients; D001205:Ascorbic Acid