title: A Markov chain Monte Carlo technique for identification of combinations of allelic variants underlying complex diseases in humans.
abstract: In recent years, the number of studies focusing on the genetic basis of common disorders with a complex mode of inheritance, in which multiple genes of small effect are involved, has been steadily increasing. An improved methodology to identify the cumulative contribution of several polymorphous genes would accelerate our understanding of their importance in disease susceptibility and our ability to develop new treatments. A critical bottleneck is the inability of standard statistical approaches, developed for relatively modest predictor sets, to achieve power in the face of the enormous growth in our knowledge of genomics. The inability is due to the combinatorial complexity arising in searches for multiple interacting genes. Similar "curse of dimensionality" problems have arisen in other fields, and Bayesian statistical approaches coupled to Markov chain Monte Carlo (MCMC) techniques have led to significant improvements in understanding. We present here an algorithm, APSampler, for the exploration of potential combinations of allelic variations positively or negatively associated with a disease or with a phenotype. The algorithm relies on the rank comparison of phenotype for individuals with and without specific patterns (i.e., combinations of allelic variants) isolated in genetic backgrounds matched for the remaining significant patterns. It constructs a Markov chain to sample only potentially significant variants, minimizing the potential of large data sets to overwhelm the search. We tested APSampler on a simulated data set and on a case-control MS (multiple sclerosis) study for ethnic Russians. For the simulated data, the algorithm identified all the phenotype-associated allele combinations coded into the data and, for the MS data, it replicated the previously known findings.
authors.affiliation: Bioinformatics Laboratory, GosNIIGenetika, Fersmana St. 3-1-31, Moscow 117545, Russia. favorov@sensi.org
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Favorov
authors.forename: Timophey V
authors.initials: TV
authors.lastname: Andreewski
authors.forename: Marina A
authors.initials: MA
authors.lastname: Sudomoina
authors.forename: Olga O
authors.initials: OO
authors.lastname: Favorova
authors.forename: Giovanni
authors.initials: G
authors.lastname: Parmigiani
authors.forename: Michael F
authors.initials: MF
authors.lastname: Ochs
mesh_terms: D000465:Algorithms; D000483:Alleles; D001499:Bayes Theorem; D003198:Computer Simulation; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006801:Humans; D040582:Inheritance Patterns / Q000235:genetics*; D008390:Markov Chains; D008957:Models, Genetic; D009010:Monte Carlo Method; D009103:Multiple Sclerosis / Q000235:genetics