[A method for construction of restriction cDNA library].
A kind of cDNA library constructed according to Restriction Display PCR(RD-PCR) technology setup in our lab,which was called restriction cDNA library,was introduced in this paper. In the construction of cDNA library, cDNA was digested with restriction endonuclease, linked with special adaptor, amplified with PCR in groups. Each group of the restriction cDNA library contained special cDNAs. The method greatly reduced the repetitive frequency and accelerated the speed of identification.
Zhu
Ji
J
Department of Bioengincering, South China University of Technology, Guangzhou 510640, China.
Ma
Wen-Li
WL
Li
Ling
L
Yao
Ru-Hua
RH
Zheng
Wen-Ling
WL