Relationship between IL-1beta gene polymorphism and gastric mucosal IL-1beta levels in patients with Helicobacter pylori infection.
BACKGROUND
Interkeukin-1 (IL-1) gene cluster polymorphisms that are thought to enhance the production of IL-1beta are associated with an increased risk of gastric cancer. To determine the role of host genetic factors in Helicobacter pylori infection, we examined the relationship between gastric mucosal IL-1beta levels and IL-1B polymorphisms in patients with H. pylori infection.


METHODS
Biopsy tissues obtained from 99 patients were homogenized and gastric mucosal IL-1beta levels were measured by enzyme-linked immunosorbent assay (ELISA). Single-base polymorphisms at positions -511 and -31 in IL-1B were analyzed.


RESULTS
The IL-1beta level in the antrum was significantly higher in genotype IL-1B-511C/C than in H. pylori-negative patients (P < 0.05). The IL-1B polymorphism did not influence the degree of gastric neutrophil and mononuclear cell infiltration, or gastric atrophy. IL-1beta levels in the corpus, but not those in the antrum, correlated to the severity of gastric atrophy.


CONCLUSIONS
These findings indicate that IL-1B polymorphisms enhance IL-1beta production in the antrum; however, other factors might regulate the production of IL-1beta in the corpus of the stomach, regardless of IL-1B polymorphisms, and high IL-1beta production may be associated with the grade of gastric atrophy in the corpus mucosa in patients with H. pylori infection.
Xuan
Jiang
J
Department of Internal Medicine, Tokai University School of Medicine, Boseidai, Isehara, 259-1193, Japan.
Deguchi
Ryuzo
R
Watanabe
Sumio
S
Ozawa
Hideki
H
Urano
Tetsuya
T
Ogawa
Yoshiaki
Y
Fukuda
Ryuki
R
Kijima
Hiroshi
H
Koga
Yasuhiro
Y
Takagi
Atsushi
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001284:Atrophy; D001706:Biopsy; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry*; D016481:Helicobacter Infections / Q000235:genetics* / Q000378:metabolism*; D016480:Helicobacter pylori; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011706:Pyloric Antrum / Q000473:pathology
D007375:Interleukin-1