Modulation of cutaneous wound healing by ozone: differences between young and aged mice.
Cutaneous tissues are frequently exposed to prooxidative environments, including UV radiation and air pollutants. Among the latter, ozone (O(3)) is of particular concern because of its high and dominating presence in photochemical smog. It is well known that O(3) depletes small molecular weight antioxidants, oxidizes proteins, induces lipid peroxidation and activates cellular responses in various tissues. Using an in vivo model (SKH-1 hairless mice), the interaction between O(3) exposure (0.5ppmx6h/day) and age was examined in relation to cutaneous wound healing. Compared to younger (8 weeks) mice, older (18 months) mice exposed to O(3) (day 0 to day 9 after wounding) exhibited delayed wound closure, increased lipid peroxidation (measured as 4-HNE protein adducts) and protein oxidation (measured as carbonyls concentration) and decreased levels of P-IkappaBalpha and TGFbeta protein. These findings support the hypothesis that oxidant pollutant exposure and age interact so as to disrupt normal wound healing processes.
Lim
Yunsook
Y
Department of Internal Medicine, School of Medicine, UC Davis, CA, USA.
Phung
Anh D
AD
Corbacho
Ana M
AM
Aung
Hnin Hnin
HH
Maioli
Emanuela
E
Reznick
Abraham Z
AZ
Cross
Carroll E
CE
Davis
Paul A
PA
Valacchi
Giuseppe
G
D000375:Aging / Q000502:physiology*; D000447:Aldehydes / Q000032:analysis / Q000737:chemistry; D000818:Animals; D003432:Cross-Linking Reagents / Q000032:analysis / Q000737:chemistry; D005260:Female; D020672:I-kappa B Proteins / Q000096:biosynthesis; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D008812:Mice, Hairless; D023421:Models, Animal; D000072000:NF-KappaB Inhibitor alpha; D010126:Ozone / Q000633:toxicity*; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D012867:Skin / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D014945:Wound Healing / Q000187:drug effects*
D000447:Aldehydes; D003432:Cross-Linking Reagents; D020672:I-kappa B Proteins; C000606205:Nfkbia protein, mouse; D011506:Proteins; D016212:Transforming Growth Factor beta; D000072000:NF-KappaB Inhibitor alpha; D010126:Ozone; C027576:4-hydroxy-2-nonenal