Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines.
The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT-PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic polymer peptides from this protein produced antibodies specifically recognizing a 25-kDa band in mycobacterial sonicate. U937 and A549 cells were used in binding assays involving 20-amino-acid-long synthetic peptides covering the whole Rv2536 protein sequence. Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY180) presented high specific binding to both types of cells; the binding was saturable and presented nanomolar affinity constants. Cross-linking assays revealed that this peptide specifically binds to 50 kDa U937 cell membrane and 45 kDa A549 cell membrane proteins.
García
Javier
J
Fundación Instituto de Inmunologia de Colombia and the Universidad Nacional de Colombia, Avda. Calle 26 No. 5000, Bogotá, Colombia 020304. javgar22@hotmail.com
Puentes
Alvaro
A
Rodríguez
Luis
L
Ocampo
Marisol
M
Curtidor
Hernando
H
Vera
Ricardo
R
Lopez
Ramses
R
Valbuena
John
J
Cortes
Jimena
J
Vanegas
Magnolia
M
Barrero
Carlos
C
Patarroyo
Manuel A
MA
Urquiza
Mauricio
M
Patarroyo
Manuel E
ME
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line / Q000378:metabolism*; D002942:Circular Dichroism; D003432:Cross-Linking Reagents; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007106:Immune Sera; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000235:genetics*; D011817:Rabbits; D020298:U937 Cells
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D007106:Immune Sera; D008565:Membrane Proteins