[Studies of DNA polymorphism at D7S21 locus in Hebei Han population].
To study the polymorphism at D7S21 locus in Hebei Han population, 124 unrelated individuals were detected rapidly by Minisatellite Variant Repeat-Polymerase Chain Reaction (MVR-PCR) and polyacrylamide gradient gel electrophoresis followed by silver staining,and digital codes were obtained. About 36 digital codes were obtained from each individual. No two unrelated individuals shared the same codes. The probability of identity in 36 digital codes was 3.48 x 10(-18). The percentage of three repeat units, a-type, t-type and 0-type was 48.5%, 49.4% and 2.1% respectively. The heterozygosity (H), excluding probability of paternity (EPP)and polymorphism information content (PIC) were 0.9876, 0.9746 and 0.9872 respectively. The results suggested that D7S21 locus has highly polymorphism in Hebei Han population. The method-polyacrylamide gradient gel electrophoresis followed by silver staining was simple,rapid and practical.
Cong
Bin
B
Department of molecular biology, Hebei Medical University, Shijiazhuang, 050017, China. congbin@263.net
Chai
Yang-Cheng
YC
Guo
Xiao-Qing
XQ
Peng
Yu-Cong
YC
Yao
Yu-Xia
YX
Wang
Jun-Xia
JX
You
Hong-Yu
HY