Effects of mushroom tyrosinase on anisaldehyde.
Anisaldehyde (p-methoxybenzaldehyde) was previously reported to inhibit the tyrosinase-catalyzed oxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) noncompetitively as long as the enzyme activity was monitored by measuring dopachrome formation. However, anisaldehyde did not inhibit this oxidation if a longer reaction time was observed, although it suppressed the initial rate of oxidation to a certain extent. Anisaldehyde significantly suppressed the rate of enzymatic oxidation of L-tyrosine.
Ha
Tae Joung
TJ
Department of Environmental Science, Policy, and Management, University of California, Berkeley, California 94720-3112, USA.
Tamura
Sadaaki
S
Kubo
Isao
I
D000363:Agaricales / Q000201:enzymology*; D001547:Benzaldehydes / Q000378:metabolism / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D007980:Levodopa / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D010084:Oxidation-Reduction
D001547:Benzaldehydes; D004791:Enzyme Inhibitors; D007980:Levodopa; C024896:4-anisaldehyde; D014442:Monophenol Monooxygenase