title: Organization of the CTX prophage in environmental isolates of Vibrio mimicus.
abstract: The organization of the CTX prophage in environmental strains of Vibrio mimicus was investigated. Sixteen hundred non-sucrose fermenting vibrios were examined for ctx gene by hybridization. Out of 1,600 isolates, 6 V. mimicus isolates contained ctxA gene. The organization of CTX prophage was determined by RFLP using ctxA probe. The CTX prophage integrated at a single site in V. mimicus genome which was present as a single copy flanked by at least a single RS element. Ribotype pattern revealed that a particular clone of V. mimicus acquired the CTXPhi in the aquatic environment. This study demonstrated that V. mimicus could act as a reservoir of CTXPhi in the aquatic environment.
authors.affiliation: International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh. sislam@icddrb.org
authors.forename: Mohammad Sirajul
authors.initials: MS
authors.lastname: Islam
authors.forename: Mohammed Ziaur
authors.initials: MZ
authors.lastname: Rahman
authors.forename: Sirajul Islam
authors.initials: SI
authors.lastname: Khan
authors.forename: Zahid Hayat
authors.initials: ZH
authors.lastname: Mahmud
authors.forename: Thandavarayan
authors.initials: T
authors.lastname: Ramamurthy
authors.forename: Gopinath Balakrish
authors.initials: GB
authors.lastname: Nair
authors.forename: Richard Bradley
authors.initials: RB
authors.lastname: Sack
authors.forename: David Allen
authors.initials: DA
authors.lastname: Sack
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D004783:Environmental Microbiology; D039002:Prophages / Q000235:genetics* / Q000302:isolation & purification; D014733:Vibrio / Q000302:isolation & purification / Q000472:pathogenicity / Q000821:virology*
chemical_list: D004279:DNA, Viral