title: Myonuclear addition to uninjured laryngeal myofibers in adult rabbits.
abstract: OBJECTIVE
  In normal mature limb skeletal muscle, satellite cells are quiescent and myonuclei do not divide after formation of their associated myofibers in the absence of injury. The possibility of myonuclear addition in uninjured laryngeal myofibers of adult rabbits was investigated in an immunohistochemical pilot study.
  
  
  METHODS
  Bromodeoxyuridine (brdU), a marker for cell division, was administered by intraperitoneal injection over a 12-hour period in rabbits. The number of brdU-positive myonuclei per myofiber was determined on cross sections through the thyroarytenoid (TA) and posterior cricoarytenoid (PCA) muscles.
  
  
  RESULTS
  In the TA muscle, 0.13% +/- 0.03% (mean +/- SEM) of the myofibers counted had a brdU-positive nucleus. In the PCA muscle, 0.13% +/- 0.01% of the myofibers counted had a brdU-positive nucleus. Approximately 0.2% and 0.3% of the myofibers of the TA and PCA muscles, respectively, had brdU-positive satellite cells associated with them. Tibialis anterior and pectoralis major muscle controls were negative for brdU-positive myonuclei.
  
  
  CONCLUSIONS
  These data support the possibility of continuous remodeling in uninjured adult laryngeal myofibers and accentuate the distinct nature of laryngeal muscle relative to limb skeletal muscle in the rabbit model.
authors.affiliation: Department of Otolaryngology, University of Minnesota, Minneapolis, Minnesota, USA.
authors.forename: George S
authors.initials: GS
authors.lastname: Goding
authors.forename: Kais I
authors.initials: KI
authors.lastname: Al-Sharif
authors.forename: Linda K
authors.initials: LK
authors.lastname: McLoon
mesh_terms: D000818:Animals; D007821:Laryngeal Muscles / Q000473:pathology*; D018485:Muscle Fibers, Skeletal / Q000473:pathology*; D011817:Rabbits; D032496:Satellite Cells, Skeletal Muscle / Q000473:pathology