Effects of acupuncturing Tsusanli (ST36) on expression of nitric oxide synthase in hypothalamus and adrenal gland in rats with cold stress ulcer.
OBJECTIVE
To study the protective effect of acupuncturing Tsusanli (S(T)36) on cold stress ulcer, and the expression of nitric oxide synthase (NOS) in hypothalamus and adrenal gland.


METHODS
Ulcer index in rats and RT-PCR were used to study the protective effect of acupuncture on cold stress ulcer, and the expression of NOS in hypothalamus and adrenal gland. Images were analyzed with semi-quantitative method.


RESULTS
The ulcer index significantly decreased in rats with stress ulcer. Plasma cortisol concentration was up regulated during cold stress, which could be depressed by pre-acupuncture. The expression of NOS1 in hypothalamus increased after acupuncture. The increased expression of NOS2 was related with stress ulcer, which could be decreased by acupuncture. The expression of NOS3 in hypothalamus was similar to NOS2, but the effect of acupuncture was limited. The expression of NOS2 and NOS3 in adrenal gland increased after cold stress, only the expression of NOS1 could be repressed with acupuncture. There was no NOS2 expression in adrenal gland in rats with stress ulcer.


CONCLUSIONS
The protective effect of acupuncturing Tsusanli (S(T)36) on the expression of NOS in hypothalamus and adrenal gland can be achieved.
Sun
Jin-Ping
JP
Emergency Neurology Department, Affiliated Hospital of Qingdao University Medical College, Shandong Province, China.
Pei
Hai-Tao
HT
Jin
Xiang-Lan
XL
Yin
Ling
L
Tian
Qing-Hua
QH
Tian
Shu-Jun
SJ
D026881:Acupuncture / Q000379:methods*; D000311:Adrenal Glands / Q000502:physiology*; D000818:Animals; D003080:Cold Temperature; D015971:Gene Expression Regulation, Enzymologic; D007031:Hypothalamus / Q000502:physiology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D013312:Stress, Physiological / Q000150:complications* / Q000503:physiopathology
D019001:Nitric Oxide Synthase