title: Expression and regulation of Sprouty-2 in the granulosa-lutein cells of the corpus luteum.
abstract: Growth factor signalling has important modulatory roles in the process of human follicular growth, oocyte maturation and corpus luteum (CL) formation. Recently, Sprouty-2, an inhibitor of receptor tyrosine kinase (RTK) signalling pathway was advocated as a marker of oocyte competence in the bovine ovary. We sought to study Sprouty-2 expression and regulation in the human ovary. RT-PCR was used to detect Sprouty-2 mRNA in human granulosa-lutein cells (GLC) collected from follicular aspiration of IVF patients. The effect of epidermal and fibroblast growth factors (EGF and FGF) on Sprouty-2 mRNA expression in GLC was studied using quantitative real-time PCR. Immunohistochemistry was performed on cultured GLC, human CL and stimulated rat ovary sections. Sprouty-2 mRNA was expressed in human GLC. EGF and basic FGF, but not FGF4 and FGF10, increased Sprouty-2 mRNA expression in GLC. The Sprouty protein was localized to GLC of early and late human CL but not to the theca cell layer. Immunostaining of developing rat CL confirmed the temporal and spatial expression of Sprouty in humans. The detection of Sprouty-2 mRNA and protein in human GLC may suggest a role for Sprouty-2 during the final stages of follicle maturation and CL formation.
authors.affiliation: Department of Obstetrics and Gynecology, Hadassah University Medical Center, The Hebrew University, Jerusalem, Israel.
authors.forename: Ronit
authors.initials: R
authors.lastname: Haimov-Kochman
authors.forename: Amir
authors.initials: A
authors.lastname: Ravhon
authors.forename: Diana
authors.initials: D
authors.lastname: Prus
authors.forename: Caryn
authors.initials: C
authors.lastname: Greenfield
authors.forename: Zvesdana
authors.initials: Z
authors.lastname: Finci-Yeheskel
authors.forename: Debra
authors.initials: D
authors.lastname: S Goldman-Wohl
authors.forename: Shira
authors.initials: S
authors.lastname: Natanson-Yaron
authors.forename: Reuven
authors.initials: R
authors.lastname: Reich
authors.forename: Simcha
authors.initials: S
authors.lastname: Yagel
authors.forename: Arye
authors.initials: A
authors.lastname: Hurwitz
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D003338:Corpus Luteum / Q000166:cytology / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000502:physiology; D005260:Female; D005346:Fibroblast Growth Factors / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008184:Luteal Cells / Q000378:metabolism*; D008565:Membrane Proteins; D008875:Middle Aged; D006080:Ovarian Follicle / Q000502:physiology; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; C485561:SPRY2 protein, human; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor