title: Evaluations of blood compatibility via protein adsorption treatment of the vascular scaffold surfaces fabricated with polylactide and surface-modified expanded polytetrafluoroethylene for tissue engineering applications.
abstract: Blood compatibility was evaluated by short-term in vitro blood perfusion on candidate vascular scaffold surfaces of a biodegradable, porous polylactide scaffold and a chemically surface-modified expanded polytetrafluoroethylene (ePTFE) over a clinical ePTFE, by measuring blood cell adhesion either directly or after adsorption treatment with albumin and fibrinogen. The results indicated that the extent of blood cell adhesion was affected by scaffold surface properties and pre-adsorption of proteins such as fibrinogen and albumin. Surface morphologies and porosity of the scaffolds were characterized by scanning electron microscopy and porosimetry, and the amount of fibrinogen and albumin adsorbed on the scaffolds was measured and verified by employing radiolabeled C(14) albumin and I(125) fibrinogen by a scintillation counter and a gamma counter, respectively. Even though treatment of fibrinogen adsorption on the samples in advance led to higher induction of blood cell adhesion than those with no fibrinogen adsorption, the polylactide scaffold surface itself induced highest amount of the adhered blood cells in this study judged by analyses of their surface area. These results would be employed as guidance in determining a choice of the implant methods, in vitro versus in vivo tissue engineering, of the novel chemically modified ePTFE and the biodegradable polylactide scaffolds.
authors.affiliation: Chemical Engineering Department, Seoul National University of Technology, Gongnung 2-dong 172, Nowon-Gu, Seoul 139-743, Korea.
authors.forename: Yoon Jeong
authors.initials: YJ
authors.lastname: Choi
authors.forename: Sung Kwang
authors.initials: SK
authors.lastname: Choung
authors.forename: Choong Man
authors.initials: CM
authors.lastname: Hong
authors.forename: In Soo
authors.initials: IS
authors.lastname: Shin
authors.forename: Sue Nie
authors.initials: SN
authors.lastname: Park
authors.forename: Seung Hwa
authors.initials: SH
authors.lastname: Hong
authors.forename: Han Ki
authors.initials: HK
authors.lastname: Park
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Park
authors.forename: Youngsook
authors.initials: Y
authors.lastname: Son
authors.forename: Insup
authors.initials: I
authors.lastname: Noh
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001769:Blood; D004285:Dogs; D010949:Plasma; D011091:Polyesters / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry; D011506:Proteins; D013057:Spectrum Analysis; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011506:Proteins; C033616:poly(lactide); D011138:Polytetrafluoroethylene