Tracking and characterisation of transfused platelets by two colour, whole blood flow cytometry.
We describe a flow cytometric technique to study transfused platelets in patients. By selecting donor/recipient pairs discrepant for HLA-A2, transfused platelets were identified using anti-HLA-A2 with a detection limit of <5%, and accuracy within 4.3% of predicted (r2 > 0.96, P < 0.0001). In two of three episodes, transfused platelets were present in greater numbers than predicted from increment counts. Platelets with bound fibrinogen fell from 20.9 +/- 23.6% of donor platelets pretransfusion to 1.7 +/- 0.3% 1 h post-transfusion, whereas P-selectin-positive platelets fell gradually, from 24.1 +/- 6.7 to 7.3 +/- 3.3% at 6 h. This method avoids radio or chemical labelling, and could be used to assess novel platelet preparations post-transfusion.
Hughes
Darren L
DL
Department of Haematology, University of Cambridge, Cambridge, UK.
Evans
Gillian
G
Metcalfe
Paul
P
Goodall
Alison H
AH
Williamson
Lorna M
LM
D000911:Antibodies, Monoclonal / Q000032:analysis; D001792:Blood Platelets / Q000276:immunology / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation; D017713:Platelet Transfusion; D011485:Protein Binding; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D015789:HLA-A2 Antigen; D019007:P-Selectin; D005340:Fibrinogen