Hydrogel optimization for cultured elastic chondrocytes seeded onto a polyglycolic acid scaffold.
The purpose of this study was to compare the effect of different hydrogels on the production of tissue-engineered cartilage based on polyglycolic acid (PGA). Chondrocytes were isolated from adult sheep auricles. Alginate, Type I collagen, methylcellulose, and pluronic F127 hydrogels were evaluated, as were controls prepared without hydrogels. Proliferated chondrocytes were mixed with each hydrogel at 20 x 10(6) cells/mL and seeded onto PGA (1 x 1 x 0.2 cm, n = 60). The constructs were cultured with serum-free medium containing 5 ng/mL TGF-beta(2) and 5 ng/mL des(1-3)IGF-I in rotational bioreactors for up to 6 weeks. The cellular morphology, histology, and biochemistry were analyzed. Type I collagen, methylcellulose, and pluronic F127 displayed improved cartilage matrix deposition in terms of histology and biochemistry compared to alginate. It was not concluded that the combined seeding of chondrocytes and hydrogels on a PGA scaffold had significantly better effects than cell seeding without hydrogels. However, the histology and other useful findings in this ECM analyses suggested that Type I collagen and MC hydrogels were the best candidates for cartilage regeneration, because of their stimulation for chondrocyte proliferation in a three-dimensional culture as well as cartilage regeneration.
Terada
Shinichi
S
Laboratory for Tissue Engineering and Organ Fabrication, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA.
Yoshimoto
Hiroshi
H
Fuchs
Julie R
JR
Sato
Michio
M
Pomerantseva
Irina
I
Selig
Martin K
MK
Hannouche
Didier
D
Vacanti
Joseph P
JP
D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000648:ultrastructure; D024042:Collagen Type I; D024043:Collagen Type II; D020100:Hydrogels; D046529:Microscopy, Electron, Transmission; D011100:Polyglycolic Acid; D012756:Sheep
D024042:Collagen Type I; D024043:Collagen Type II; D020100:Hydrogels; D011100:Polyglycolic Acid