title: Self-made frits for nanoscale columns in proteomics.
abstract: We report here the production of self-made frits for nano-columns. The frits introduce a minor dead volume and can be placed in capillaries with a wide range of diameters (20-250 microm tested) in an extremely simple and low-cost procedure. The obtained columns appear to be comparable to "no-frit" columns with near-ideal chromatographic characteristics. We expect that this frit will be useful for the spotting of gradients onto MALDI plates but also where special ESI set-ups do not allow for "no-frit" solutions.
authors.affiliation: The FIRC Institute of Molecular Oncology Foundation, Milan, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Maiolica
authors.forename: Dario
authors.initials: D
authors.lastname: Borsotti
authors.forename: Juri
authors.initials: J
authors.lastname: Rappsilber
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation*; D002868:Chromosomal Proteins, Non-Histone; D013058:Mass Spectrometry / Q000295:instrumentation; D036103:Nanotechnology / Q000295:instrumentation*; D040901:Proteomics / Q000295:instrumentation; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014157:Transcription Factors
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D014157:Transcription Factors