title: [Investigation of immunological mechanism of the in vivo anti-tumor effect of K8 (C6H11N3O2)2[SiW9Ti3O40]] (WT)].
abstract: OBJECTIVE
  To investigate the in vivo anti-tumor effect of K(8)(C(6)H(11)N(3)O(2))(2)[SiW(9)Ti(3)O(40)] (WT) and the immunological mechanism.
  
  
  METHODS
  WT was administrated intragastrically to mice bearing H22 hepatocarcinoma for 10 days and then the tumors were isolated and weighed. MTT colorimetry was used to detect lymphocyte transformation function and cytotoxicity of NK cells. The morphology of apoptotic tumor cells was observed under phase contrast microscope and light microscope after HE staining. The apoptosis rate of BEL-7402 cells treated with WT was detected by flow cytometry.
  
  
  RESULTS
  Compared to the control group, WT obviously inhibited the growth of H22 tumor in tumor bearing mice (P<0.05). It enhanced significantly lymphocyte transformation function and cytotoxicity of NK cells (P<0.05). Apoptotic tumor cells were observed under microscope and detected by flow cytometry.
  
  
  CONCLUSIONS
  WT inhibits significantly the growth of tumor by up-regulating the activity of immunocytes and inducing apoptosis of tumor cells.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Song-ling
authors.initials: SL
authors.lastname: Zhang
authors.forename: Shao-lin
authors.initials: SL
authors.lastname: Mei
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Chang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000276:immunology*; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008858:Microscopy, Phase-Contrast
chemical_list: D000970:Antineoplastic Agents