Development of direct size-exclusion chromatography separation for the determination of molar mass of native procyanidins in the phenolate form.
A size-exclusion chromatographic method using an aqueous solvent as eluent was developed for the determination of the molecular mass of procyanidins has been developed. Size-exclusion chromatography is a chromatographic technique used to determine molecular weight distribution of polymers. During the development work, PL aquagel-OH columns and several different eluent compositions were investigated. The best separation of procyanidins was obtained with an eluent consisting of water-methanol (50:50, v/v), NaNO3: 0.18 M, pH < pKa + 2. The results from this aqueous SEC method were compared with results obtained using SEC with organic solvents.
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Tonnellerie Demptos,Centre d'Etude Structurale et d'Analyse des Molećules Organiques, Université Bordeaux I, 351 Cours de la Libération, Talence F-33405, France. n.vivas@cesamo.u-bordeaux1.fr
Vivas
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Gaulejac de Vivas
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Fouquet
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D002138:Calibration; D002850:Chromatography, Gel / Q000379:methods*; D008970:Molecular Weight; D044945:Proanthocyanidins / Q000737:chemistry / Q000302:isolation & purification*; D027843:Vitis / Q000737:chemistry
D044945:Proanthocyanidins