title: Detection of human metabolites using multi-capillary columns coupled to ion mobility spectrometers.
abstract: The human breath contains indicators of human health and delivers information about different metabolism processes of the body. The detection and attribution of these markers provide the possibility for new, non-invasive diagnostic methods. In the recent study, ion mobility spectrometers are used to detect different volatile organic metabolites in human breath directly. By coupling multi-capillary columns using ion mobility spectrometers detection limits down to the ng/L and pg/L range are achieved. The sampling procedure of human breath as well as the detection of different volatiles in human breath are described in detail. Reduced mobilities and detection limits for different analytes occurring in human breath are reported. In addition, spectra of exhaled air using ion mobility spectrometers obtained without any pre-concentration are presented and discussed in detail. Finally, the potential use of IMS with respect to lung infection diseases will be considered.
authors.affiliation: ISAS, Institute for Analytical Sciences, Bunsen-Kirchhoff-Str. 11, 44139 Dortmund, Germany. ruzsanyi@ansci.de
authors.forename: Vera
authors.initials: V
authors.lastname: Ruzsanyi
authors.forename: Jörg Ingo
authors.initials: JI
authors.lastname: Baumbach
authors.forename: Stefanie
authors.initials: S
authors.lastname: Sielemann
authors.forename: P
authors.initials: P
authors.lastname: Litterst
authors.forename: M
authors.initials: M
authors.lastname: Westhoff
authors.forename: Lutz
authors.initials: L
authors.lastname: Freitag
mesh_terms: D001944:Breath Tests / Q000379:methods*; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007659:Ketones / Q000032:analysis; D008660:Metabolism; D009862:Online Systems; D009930:Organic Chemicals / Q000032:analysis*; D018410:Pneumonia, Bacterial / Q000503:physiopathology; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D014835:Volatilization
chemical_list: D007659:Ketones; D009930:Organic Chemicals