Perlecan from human epithelial cells is a hybrid heparan/chondroitin/keratan sulfate proteoglycan.
Perlecan is a multidomain proteoglycan, usually substituted with heparan sulphate (HS), and sometimes substituted with both HS and chondroitin sulphate (CS). In this paper, we describe perlecan purified from HEK-293 cells substituted with HS, CS and keratan sulphate (KS). KS substitution was confirmed by immunoreactivity with antibody 5D4, sensitivity to keratanase treatment, and fluorophore-assisted carbohydrate electrophoresis. HEK-293 perlecan failed to promote FGF-dependent cell growth in an in vitro assay. This study is the first to report perlecan containing KS, and makes perlecan one of only a very few proteoglycans substituted with three distinct types of glycosaminoglycan chains.
Knox
S
S
Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA.
Fosang
A J
AJ
Last
K
K
Melrose
J
J
Whitelock
J
J
D002460:Cell Line; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000737:chemistry*; D005346:Fibroblast Growth Factors / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007632:Keratan Sulfate / Q000032:analysis* / Q000378:metabolism; D000071234:Lumican
D011508:Chondroitin Sulfate Proteoglycans; D019812:Heparan Sulfate Proteoglycans; C000604306:LUM protein, human; D000071234:Lumican; C071927:perlecan; D005346:Fibroblast Growth Factors; D007632:Keratan Sulfate