title: Polymorphisms in 16S rRNA genes of Flavobacterium psychrophilum correlate with elastin hydrolysis and tetracycline resistance.
abstract: Flavobacterium psychrophilum is the etiological agent of bacterial coldwater disease, which causes significant problems to aquaculture worldwide. A recent study (Soule M, Cain K, LaFrentz S, Call DR [2005] Infect Immun 73:3799-3802) identified two 16S rRNA gene sequence variants (6 base differences) within the variable stem-loop region 3 for F. psychrophilum strains ATCC 49418 and CSF 259-93. That study also hypothesized that F. psychrophilum is composed of at least 2 distinct genetic lineages (I and II) described by a microarray-based comparative genomics study. In the present study, we augmented an existing 16S rDNA microarray to detect both 16S rRNA sequence variants from F. psychrophilum. Subsequent microarray experiments showed that CSF 259-93 hybridized as expected, but ATCC 49418 was positive for both sequence variants. We then developed a PCR-restriction fragment length polymorphism (RFLP) assay (MnlI and MaeIII) to distinguish between the 2 sequences. Gel isolation of PCR-RFLP products, cloning, and sequencing confirmed that ATCC 49418 harbors both 16S rRNA sequences. Microarray experiments showed that 11 of 14 strains from genetic Lineage I harbor both the CSF 259-93 and ATCC 49418 16S rRNA sequence variants, whereas all 15 Lineage II strains were only positive for the CSF 259-93 sequence (p < 0.0001). Elastin hydrolysis and tetracycline resistance were most closely associated with the latter strains (p < 0.0001 and p = 0.024, respectively). These data support the hypothesis that F. psychrophilum is composed of at least 2 distinct genetic lineages that are closely associated with host origin.
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040, USA.
authors.forename: Marilyn
authors.initials: M
authors.lastname: Soule
authors.forename: Stacey
authors.initials: S
authors.lastname: LaFrentz
authors.forename: Kenneth
authors.initials: K
authors.lastname: Cain
authors.forename: Scott
authors.initials: S
authors.lastname: LaPatra
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Call
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004351:Drug Resistance / Q000235:genetics*; D004549:Elastin / Q000378:metabolism*; D005417:Flavobacterium / Q000235:genetics* / Q000378:metabolism; D006868:Hydrolysis; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013752:Tetracycline / Q000378:metabolism*
chemical_list: D012336:RNA, Ribosomal, 16S; D004549:Elastin; D013752:Tetracycline