Effects of formaldehyde inhalation on lung of rats.
OBJECTIVE
To analyze protein changes in the lung of Wistar rats exposed to gaseous formaldehyde (FA) at 32-37 mg/m3 for 4 h/day for 15 days using proteomics technique.


METHODS
Lung samples were solubilized and separated by two-dimensional electrophoresis (2-DE), and gel patterns were scanned and analyzed for detection of differently expressed protein spots. These protein spots were identified by MALDI-TOF-MS and NCBInr protein database searching.


RESULTS
Four proteins were altered significantly in 32-37 mg/m3 FA group, with 3 proteins up-regulated, 1 protein down-regulated. The 4 proteins were identified as aldose reductase, LIM protein, glyceraldehyde-3-phosphate dehydrogenase, and chloride intracellular channel 3.


CONCLUSIONS
The four proteins are related to cell proliferation induced by FA and defense reaction of anti-oxidation. Proteomics is a powerful tool in research of environmental health, and has prospects in search for protein markers for disease diagnosis and monitoring.
Yang
Yu-Hua
YH
Institute of Health and Environmental Medicine, Tianjin 300050, China. anniyang001@sohu.com
Xi
Zhu-Ge
ZG
Chao
Fu-Huan
FH
Yang
Dan-Feng
DF
D000280:Administration, Inhalation; D000818:Animals; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005557:Formaldehyde / Q000633:toxicity*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011506:Proteins; D005557:Formaldehyde