title: [Effect of intranasal interleukin-12 gene therapy for allergic rhinitis in murine model].
abstract: OBJECTIVE
  To investigate whether the local application of IL-12 gene with EBV-plasmid vector to nasal mucosa could prevent allergic inflammation in murine allergic rhinitis model.
  
  
  METHODS
  Thirty-six BALB/C mice were randomly divided into allergic rhinitis group gene therapy group and control group. In mice with OVA-induced allergic rhinitis, the EBV/lipoplex (a novel cationic lipid combined with EBV-plasmid vector, pGEG. mIL-12) was locally administered into nasal mucosa before OVA challenge. The expression of IL-12 mRNA and protein, the change of eosinophilia and mast cell, and Th2 cytokine production in the nasal mucosa were measured.
  
  
  RESULTS
  The amounts of IL-12 mRNA positive cells and IL-12 positive cells in nasal mucosa of gene therapy group were significantly higher than that of allergic rhinitis group (P < 0.01 and P < 0.05). The amount of eosinophils, mast cells, and the level of IL-5 expression in nasal mucosa in allergic rhinitis group were significantly higher than those in gene therapy group and control group (P < 0.01). The level of total IgE of peripheral blood in allergic rhinitis group was significantly higher than that in gene therapy group and control group (F = 1216.21, P < 0.01).
  
  
  CONCLUSIONS
  These findings indicated that IL-12 mRNA and protein were expressed effectively after the local administration of pGEG. mIL-12 in the nasal mucosa. The local application of pGEG. mIL-12 is effective in modulating nasal allergic response and may be a convenient method for future approach to allergic rhinitis.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Beijing Tongren Hospital Affiliated to the Capital University of Medical Sciences, Beijing 100730, China. entzhou@263.net
authors.forename: Bing
authors.initials: B
authors.lastname: Zhou
authors.forename: De-min
authors.initials: DM
authors.lastname: Han
authors.forename: Tong
authors.initials: T
authors.lastname: Wang
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Wang
authors.forename: Er-zhong
authors.initials: EZ
authors.lastname: Fan
authors.forename: Zhong-yan
authors.initials: ZY
authors.lastname: Liu
mesh_terms: D000818:Animals; D015316:Genetic Therapy; D005822:Genetic Vectors; D018664:Interleukin-12 / Q000235:genetics / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000378:metabolism; D012221:Rhinitis, Allergic, Perennial / Q000628:therapy*
chemical_list: D018664:Interleukin-12