title: Long form leptin receptor mRNA expression in the hypothalamus and pituitary during early pregnancy in the pig.
abstract: OBJECTIVE
  The aim of this study was the detection and location of long form leptin receptor (OB-Rb) in different area of hypothalamus and pituitary in the pig during early pregnancy.
  
  
  METHODS
  Expression of OB-Rb was examined by RT-PCR in the different area of hypothalamus: medial basal hypothalamus (MBH), preoptic area (POA), stalk median eminence (SME), as well as pituitary: the anterior (AP) and posterior (NP) lobe collected from gilts at days 14-16 (n=4) and 30-32 (n=4) of pregnancy.
  
  
  RESULTS
  The results showed that OB-Rb mRNA was expressed in the hypothalamus (MBH, POA and SME), pituitary (AP, NP) and adipose tissue in the pig during early pregnancy (at days 14-16 and 30-32).
  
  
  CONCLUSIONS
  These findings support the idea that leptin might play a role in the regulation of the hypothalamic-pituitary axis activity, and consequently in the control of pregnancy during critical period of embryo implantation in the pig.
authors.affiliation: Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo, Poland. gabriela.siawrys@uwm.edu.pl
authors.forename: Gabriela
authors.initials: G
authors.lastname: Siawrys
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Przala
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Kaminski
authors.forename: Nina
authors.initials: N
authors.lastname: Smolinska
authors.forename: Alina
authors.initials: A
authors.lastname: Gajewska
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Kochman
authors.forename: Mariusz
authors.initials: M
authors.lastname: Skowronski
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Staszkiewicz
mesh_terms: D000818:Animals; D005260:Female; D007031:Hypothalamus / Q000502:physiology*; D007033:Hypothalamus, Middle / Q000502:physiology; D008473:Median Eminence / Q000502:physiology; D010903:Pituitary Gland, Anterior / Q000502:physiology*; D010904:Pituitary Gland, Posterior / Q000502:physiology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011301:Preoptic Area / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000235:genetics*; D054411:Receptors, Leptin; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
chemical_list: D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin