Exploration of the global antioxidant capacity of the stratum corneum by cyclic voltammetry.
Cyclic voltammetry is proposed as a new method for evaluating the antioxidant capacity of skin based on the reducing properties of low molecular weight antioxidants (LMWA). Experiments were performed simply by recording the anodic current at 0.9 V/SCE of a platinum microelectrode placed directly on the epidermis surface without any gel or water. This method ensured a direct, rapid (less than 1 min), reliable (accuracy 12%) and non-invasive measurement of the global antioxidant capacity of the stratum corneum with a high spatiotemporal resolution. At the same time, the pH of the skin surface was determined by recording the cathodic current at 0 V/SCE. Based on an exploratory study involving nine volunteer subjects, the evolution of the amperometric response of the microelectrode with time revealed a periodic modification of the redox properties.
Ruffien-Ciszak
Audrey
A
Laboratoire de Génie Chimique, UMR CNRS 5503, Université Paul Sabatier - ToulouseIII, 118 route de Narbonne, 31062 Toulouse, France.
Gros
Pierre
P
Comtat
Maurice
M
Schmitt
Anne-Marie
AM
Questel
Emmanuel
E
Casas
Christiane
C
Redoules
Daniel
D
D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D001205:Ascorbic Acid / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D004594:Electrophysiology; D004817:Epidermis / Q000187:drug effects* / Q000502:physiology*; D016166:Free Radical Scavengers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011199:Potentiometry / Q000379:methods*; D017382:Reactive Oxygen Species; D015203:Reproducibility of Results; D013997:Time Factors
D000975:Antioxidants; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D001205:Ascorbic Acid