Transient activation of protein phosphatase 2A induced by electroconvulsive shock in the rat frontal cortex.
We have attempted to determine the effects of electroconvulsive shock (ECS) on protein phosphatase 2A (PP2A) in the frontal cortices of rats. PP2A exhibited a 30% increase in activity immediately after ECS treatment. Immunoblot analysis revealed that phosphorylation signals, including protein kinase B (Akt/PKB), glycogen synthase kinase-3beta (GSK-3beta), and cyclic adenosine monophosphate response element binding protein (CREB) were reduced immediately after ECS treatment. When an additional ECS was administered after the activation of these kinases, the immediate reactivation of PP2A overrode the kinase activity. ECS induces transient PP2A activation prior to kinase activation, and this pattern of activity may induce the biphasic phosphorylation of substrate proteins.
Kang
Ung Gu
UG
Department of Psychiatry & Behavioral Science, Seoul National University College of Medicine, Seoul, South Korea.
Jeon
Won Je
WJ
Kim
Yeni
Y
Chung
Chun Kee
CK
Park
Joo Bae
JB
Juhnn
Yong Sung
YS
Kim
Yong Sik
YS
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D004307:Dose-Response Relationship, Radiation; D004597:Electroshock / Q000379:methods*; D004789:Enzyme Activation / Q000528:radiation effects; D005625:Frontal Lobe / Q000378:metabolism / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000528:radiation effects*; D008297:Male; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation / Q000528:radiation effects; D011494:Protein Kinases / Q000378:metabolism; D054648:Protein Phosphatase 2; D051381:Rats; D017207:Rats, Sprague-Dawley; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism; D013997:Time Factors
D013912:Threonine; D012694:Serine; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2