title: Utilization of zirconia stationary phase as a tool in drug control.
abstract: Zirconia-based stationary phases represent an interesting alternative to silica-based materials. Two zirconia-based stationary phases were studied as an option for use in drug analysis. The different properties of zirconia material, distinct from RP silica-columns, were employed for the development of a novel and rapid stability monitoring HPLC method. This method enables simultaneous control of possible degradation processes of active substance (ibuprofen) as well as antimicrobial excipients (methyl-and propylparaben). The separation of ibuprofen, its two main degradation products 2-(4-isobutyrylphenyl)propionic acid and 4-isobutylacetophenone, parabens, and 4-hydroxybenzoic acid as their degradation product was successfully accomplished on a Zr-CarbonC18 column using a mobile phase consisting of acetonitrile-phosphate buffer (pH 4.8)-propan-2-ol (27:56:17, v/v/v). Detection was performed at 258 nm and the analysis was completed within 17 minutes.
authors.affiliation: Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control, Hradec Králové, Czech Republic. radim.kucera@faf.cuni.cz
authors.forename: Radim
authors.initials: R
authors.lastname: Kucera
authors.forename: Václav
authors.initials: V
authors.lastname: Zizkovský
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Sochor
authors.forename: Jirí
authors.initials: J
authors.lastname: Klimes
authors.forename: Jirí
authors.initials: J
authors.lastname: Dohnal
mesh_terms: D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D007052:Ibuprofen / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics; D010226:Parabens / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry; D013696:Temperature; D015040:Zirconium
chemical_list: D010226:Parabens; D004364:Pharmaceutical Preparations; D015040:Zirconium; C028541:zirconium oxide; D007052:Ibuprofen