[Effect of naoyian on phosphorylated Akt in the neural stem cell injured with anoxia].
OBJECTIVE
To determine the effect of naoyian (NYA) on the expression of phosph-Akt in neural stem cells injured with anoxia.


METHODS
The hippocampal neural stem cells (NSC) taken from the Sprageu-Dawley rat brain at 5 days after birth were cultured in DMEM/F12 solution containing EGF and bFGF. After being treated with anoxia for 6 hours, the neural stem cells were incubated in solution containing the NYA serum or normal serum. The neural stem cells were then detected for phosph-Akt and TUNEL stain.


RESULTS
After the neural stem cells were injured with anoxia, the expression of the phosph-Akt in NYA serum group was higher than that in the normal serum group and the serum-free group. Treated with NYA serum, the amount of apoptotic NSC decreased.


CONCLUSIONS
After the neural stem cell injured by anoxia was treated with NYA serum, the phosphorylation of Akt increased, the apoptosis of NSC was inhibited.
Luo
Jie-kun
JK
Department of Combined Traditional Chinese and Western Medicine, Xiangya Hospital of Central South AUniversity, Changsha 410008, China.
Li
Xing-qun
XQ
Liu
Bai-yan
BY
Xiao
Lan
L
Zhang
Hua-xian
HX
D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006624:Hippocampus / Q000473:pathology*; D008297:Male; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000201:enzymology* / Q000473:pathology
D004365:Drugs, Chinese Herbal; D017346:Protein Serine-Threonine Kinases