Suspension culture of gametophytes of transgenic kelp in a photobioreactor.
Transgenic Laminaria japonica gametophytes producing a recombinant tissue-type plasminogen activator (rtPA) protein, which is an effective third-generation thrombolytic agent for acute myocardial infarction (AMI), were cultured in an illuminated bubble column bioreactor. A maximum final dry cell weight of 1120 mg l(-1) was obtained in batch culture with an initial dry cell weight of 126 mg l(-1) and with aeration rate of 1.2 l air min(-1 )l(-1) culture, nitrate at 1.5 mM: and phosphate at 0.17 mM: . The yield of rtPA was 56 microg g(-1) dry cell wt. This is the first report regarding cultivation of a transgenic macroalga in a bioreactor.
Gao
Jiangtao
J
Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, P.R. China.
Zhang
Yicheng
Y
Wang
Honghua
H
Qin
Song
S
D018533:Biomass; D019149:Bioreactors; D006801:Humans; D044423:Kelp / Q000235:genetics / Q000254:growth & development*; D007795:Laminaria / Q000235:genetics / Q000254:growth & development*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D010959:Tissue Plasminogen Activator