HPLC method for the determination of ecdysterone in extractive solution from Pfaffia glomerata.
A RP-LC method was developed and validated to quantify ecdysterone in extractive solution from subterraneous parts of Pfaffia glomerata. The analysis was performed using a RP-18 column with acetonitrile:water isocratic elution and the detection was carried out by UV at 242 nm. The standard curve for ecdysterone was linear over the range of 5.2-41.6 microg/ml (R2=0.9995). The extractive solution showed linear response in the range of 25.05-175.35 microg/ml (R2=0.9977). This method showed excellent repeatability (relative standard deviation, R.S.D.<2.0%), intermediary precision (R.S.D.=2.13%) and accuracy (101.04; R.S.D.=1.51%). The limit of detection (LOD) was 0.036 microg/ml and the limit of quantification (LOQ) was 0.110 microg/ml, demonstrating the sensitivity of the method. This assay can be readily utilized as quality controlled method for P. glomerata preparations.
Zimmer
Aline R
AR
Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, 906010-000 Porto Alegre, RS, Brazil.
Bruxel
Fernanda
F
Bassani
Valquíria L
VL
Gosmann
Grace
G
D000097:Acetonitriles; D027703:Amaranthaceae; D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004441:Ecdysterone / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014867:Water
D000097:Acetonitriles; D010936:Plant Extracts; D014867:Water; D004441:Ecdysterone; C032159:acetonitrile