title: A rapid and efficient preparation of [123I]radiopharmaceuticals using a small HPLC (Rocket) column.
abstract: A simplified method for the rapid and efficient preparation of [(123)I]radiopharmaceuticals is described. Three radiopharmaceuticals, [(123)I]beta-CIT, [(123)I]MIBG and [(123)I]clioquinol, were synthesised and purified as model compounds. The radiotracers were labelled with iodine-123 using electrophilic oxidative conditions and purified by a compact semi-preparative reverse phase column (C-18, 3 microm, 7 x 53 mm, Alltima Rocket, Alltech) using aqueous-ethanol as HPLC solvents that were directly used for radiopharmaceutical formulation. The radiochemical purity of the radioiodinated tracers as assessed by analytical HPLC was higher than 99% with specific activity higher than 3 GBq/nmol. The total preparation time of a radiotracer ranged from 40 to 60 min and, starting from 3.7 GBq of iodine-123, more than 2.5 GBq of formulated radiopharmaceuticals were available for clinical investigations.
authors.affiliation: Radiopharmaceuticals Division R&D, Australian Nuclear Science & Technology, Organisation, Menai, NSW 2234, Sydney, Australia. akx@ansto.gov.au
authors.forename: Andrew
authors.initials: A
authors.lastname: Katsifis
authors.forename: Vahan
authors.initials: V
authors.lastname: Papazian
authors.forename: Timothy
authors.initials: T
authors.lastname: Jackson
authors.forename: Christian
authors.initials: C
authors.lastname: Loc'h
mesh_terms: D019797:3-Iodobenzylguanidine / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007464:Clioquinol / Q000737:chemistry*; D003042:Cocaine / Q000031:analogs & derivatives* / Q000737:chemistry; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D019275:Radiopharmaceuticals / Q000737:chemistry*
chemical_list: D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D019797:3-Iodobenzylguanidine; C069723:2beta-carbomethoxy-3beta-(4-iodophenyl)tropane; D007464:Clioquinol; D003042:Cocaine