title: [Effect of EGCG on H2O2-induced MnSOD gene expression in cultured spiral ganglion cells].
abstract: OBJECTIVE
  To investigate the influence of EGCG on H2O2-induced gene expression of manganese superoxide dismutase (MnSOD or SOD2) in cultured spiral ganglion cells (SGCs) in vitro.
  
  
  METHODS
  SGCs were cultured in vitro, and H2O2 and/or EGCG in different concentrations were used. Semi-quantitative RT-PCR was applied to observe MnSOD gene expression in SGCs after H2O2 and EGCG treatment.
  
  
  RESULTS
  The expression of MnSOD gene was up-regulated with the increase of the concentration of H2O2 in cultured SGCs, and MnSOD gene expression was significantly up-regulated at a dose of H2O2 > or =100 micromol/L. However, this up-regulation was suppressed after simultaneously treated with 100 microg/ml EGCG.
  
  
  CONCLUSIONS
  EGCG suppresses H2O2-induced up-regulation of MnSOD gene expression in cultured SGCs by getting rid of oxygen free radicals, reinforcing the activity of antioxidant enzymes such as MnSOD, and protects cultured SGCs from H2O2-induced oxidizing damage.
authors.affiliation: Department of Otorhinolaryngology, Third Xiangya Hospital, Central South University, Changsha 410013, China. guohliu@hotmail.com
authors.forename: Guo-hui
authors.initials: GH
authors.lastname: Liu
authors.forename: Ding-hua
authors.initials: DH
authors.lastname: Xie
authors.forename: Gang-hua
authors.initials: GH
authors.lastname: Zhu
authors.forename: Wei-jing
authors.initials: WJ
authors.lastname: Wu
authors.forename: Sheng-lei
authors.initials: SL
authors.lastname: Ge
mesh_terms: D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D003051:Cochlea / Q000166:cytology / Q000294:innervation; D005260:Female; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D051379:Mice; D013136:Spiral Ganglion / Q000166:cytology / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D002392:Catechin; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate; D013482:Superoxide Dismutase