The effect of tripeptide tyroserleutide (YSL) on animal models of hepatocarcinoma.
This study aimed to observe the effects of tyroserleutide (tyrosyl-seryl-leucine, YSL) on the survival time of mice transplanted with the ascitic fluid-type hepatocarcinoma H22, as well as the inhibitory effect of tyroserleutide on the human hepatocarcinoma Bel-7402 that was transplanted into nude mice. At doses of 80, 20 and 5 microg/kg/d, tyroserleutide significantly prolonged the survival of mice transplanted with H22 tumor cells, producing survival rates of 89%, 39% and 49%, respectively, which were statistically significantly different from the saline group (P < 0.05). YSL, at doses of 80, 160 and 320 microg/kg/d significantly inhibited the growth of the human hepatocarcinoma Bel-7402 tumor in nude mice, producing inhibition of 40%, 64% and 59%, respectively; this inhibition was significantly greater than that by saline (P < 0.05). HE staining and electron microscopy of the pathological changes of the tumor in nude mice showed that YSL changed the structure Bel-7402 tumor cells that were transplanted into nude mice, and also induced tumor cell apoptosis and necrosis, which could be a mechanism by which YSL inhibits the tumor growth in animal models.
Yao
Zhi
Z
Department of Immunology, Tianjin Medical University, Tianjin 300070, China. yaozhi@tmu.cn
Lu
Rong
R
Jia
Jing
J
Zhao
Pengpeng
P
Yang
Jing
J
Zheng
Minna
M
Lu
Jing
J
Jin
Mengjue
M
Yang
Haixian
H
Gao
Wenyuan
W
D000818:Animals; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008956:Models, Chemical; D009336:Necrosis; D009368:Neoplasm Transplantation; D009842:Oligopeptides / Q000494:pharmacology*
D009842:Oligopeptides; C484773:H-Tyr-Ser-Leu-OH