title: Purification of 3-indolylacetic acid by solid phase extraction.
abstract: This paper deals with the use of solid-phase extraction (SPE) for the extraction and purification of 3-indolylacetic acid (IAA), the main and most important representative of the plant growth regulators auxins. The procedure is based on the use of C18-SPE columns in two steps. In the first one, raw extract in methanol:water (4:1) is applied on the column in order to remove neutral ballast. In the second step the eluate is diluted with water to a final methanol concentration of 20% (to decrease the elution strength of the solvent) and acidified with formic acid to a final concentration of 1% (to convert the IAA from the anionic to the neutral form). Neutral IAA is then retained on the second SPE column, eluted by (acidified) methanol, and subjected to final analysis. Scintillation counting of tritium labeled IAA standard was used for the investigation and optimization of the SPE procedure. Gas chromatography with mass spectrometric detection was suggested as a convenient method for routine determination of IAA after its methylation with diazomethane. Overall recovery of the procedure was estimated as 89-94% and a physiological level of IAA equal to 0.48 nmol/g (84 ng/g) fresh weight was found using an optimized SPE-GC-MS method.
authors.affiliation: Laboratory of Growth Regulators, Palacký University & Institute of Experimental Botany ASCR, Olomouc, Czech Republic. rolcikj@aix.upol.cz
authors.forename: Jakub
authors.initials: J
authors.lastname: Rolcík
authors.forename: Jana
authors.initials: J
authors.lastname: Recinská
authors.forename: Petr
authors.initials: P
authors.lastname: Bartak
authors.forename: Miroslav
authors.initials: M
authors.lastname: Strnad
authors.forename: Els
authors.initials: E
authors.lastname: Prinsen
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007210:Indoleacetic Acids / Q000302:isolation & purification*; D031316:Pelargonium / Q000737:chemistry; D010937:Plant Growth Regulators / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid