Higher plant RecA-like protein is homologous to RadA.
A novel RecA-like protein, differing from Dmc1 and Rad51, was characterized in Oryza sativa L. cv. Nipponbare. Because the protein is homologous to bacterial RadA, the gene was designated OsRadA. The open reading frame was predicted to encode a 66kDa protein of 619 amino acid residues and was found in plants but not animals or yeast. OsRadA showed D-loop and single-stranded DNA-dependent ATPase activities. Gene expression was found to be high in meristematic tissues, and was localized in the nucleus. An RNAi mutant of Arabidopsis thaliana RadA (AtRadA) was sensitive to mutagenic agents such as UV and MMC, suggesting that RadA functions in DNA repair.
Ishibashi
Toyotaka
T
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan.
Isogai
Minako
M
Kiyohara
Hiroyuki
H
Hosaka
Masahiro
M
Chiku
Hiroyuki
H
Koga
Asami
A
Yamamoto
Taichi
T
Uchiyama
Yukinobu
Y
Mori
Yoko
Y
Hashimoto
Junji
J
Ausió
Juan
J
Kimura
Seisuke
S
Sakaguchi
Kengo
K
D017360:Arabidopsis / Q000187:drug effects / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D019843:Archaeal Proteins / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D049109:Cell Proliferation; D003001:Cloning, Molecular; D004260:DNA Repair / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D016685:Mitomycin / Q000009:adverse effects; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000166:cytology / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D011938:Rec A Recombinases / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions; D014466:Ultraviolet Rays / Q000009:adverse effects
D029681:Arabidopsis Proteins; D019843:Archaeal Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D010940:Plant Proteins; C117252:RadA protein, archaeal; C100901:RadA protein, bacteria; D016685:Mitomycin; D011938:Rec A Recombinases