The assessment of oxygen metabolism selected parameters of blood platelets exposed to low frequency magnetic radiation in cars--in vitro studies.
OBJECTIVE
The aim of the study was to determine how free radicals generation in blood platelets exposed to electromagnetic field (EMF) occurring in cars affects the process of these morphotic elements cell membranes phospholipid peroxidation.


METHODS
The suspension of human blood platelets was exposed to EMF of proper characteristics in a specially arranged research stand. After 30, 60 and 90 min exposure of the platelet specimen to EMF, free radicals generation was measured with chemiluminescence and malondialdehyde concentration according to Placer et al. method. The obtained results were compared with the control values.


RESULTS
The increase of free radicals generation was observed after 30 and 90 min exposure of platelets to magnetic field. Malondialdehyde reached the highest values also after 30 and 90 min exposure of the platelets to EMF as compared to the control.


CONCLUSIONS
The increase in oxygen reactive species generation under the effect of exogenic magnetic radiation as well as proportional intensification of the peroxidation process determined on the basis of malondialdehyde concentration (the marker of this phenomenon) point to the platelet sensitivity to the investigated environmental factor.
Buczyński
A
A
Health Promotion and Education Department, Medical University Lódz, Poland.
Pacholski
K
K
Dziedziczak-Buczyńska
M
M
Henrykowska
G
G
Jerominko
A
A
D001792:Blood Platelets / Q000378:metabolism / Q000528:radiation effects*; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects; D004574:Electromagnetic Fields; D005609:Free Radicals / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000528:radiation effects; D010100:Oxygen / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
D005609:Free Radicals; D010743:Phospholipids; D017382:Reactive Oxygen Species; D010100:Oxygen