Mutant tamm-horsfall glycoprotein accumulation in endoplasmic reticulum induces apoptosis reversed by colchicine and sodium 4-phenylbutyrate.
As a consequence of uromodulin gene mutations, individuals develop precocious hyperuricemia, gout, and progressive renal failure. In vitro studies suggest that pathologic accumulation of uromodulin/Tamm-Horsfall glycoprotein (THP) occurs in the endoplasmic reticulum (ER), but the pathophysiology of renal damage is unclear. It was hypothesized that programmed cell death triggered by accumulation of misfolded THP in the ER causes progressive renal disease. Stably transfected human embryonic kidney 293 cells and immortalized thick ascending limb of Henle's loop cells with wild-type and mutated uromodulin cDNA were evaluated to test this hypothesis. Immunocytochemistry, ELISA, and deglycosylation studies indicated that accumulation of mutant THP occurred in the ER. FACS analyses showed a significant increase in early apoptosis signal in human embryonic kidney 293 and thick ascending limb of Henle's loop cells that were transfected with mutant uromodulin constructs. Colchicine and sodium 4-phenylbutyrate treatment increased secretion of THP from the ER to the cell membrane and into the culture media and significantly improved cell viability. These findings indicate that intracellular accumulation of THP facilitates apoptosis and that this may provide the pathologic mechanism responsible for the progressive renal damage associated with uromodulin gene mutations. Colchicine and sodium 4-phenylbutyrate reverse these processes and could potentially be beneficial in ameliorating the progressive renal damage in uromodulin-associated kidney diseases.
Choi
Sung Won
SW
NIH-NIDCR, 10 Center Drive, Building 10, Room 5-2531, Bethesda, MD 20892-1432, USA.
Ryu
Ok Hee
OH
Choi
Sun Jin
SJ
Song
In Sun
IS
Bleyer
Anthony J
AJ
Hart
Thomas C
TC
D017209:Apoptosis / Q000187:drug effects*; D001789:Blood Group Antigens / Q000235:genetics*; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology; D009088:Mucoproteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D010654:Phenylbutyrates / Q000494:pharmacology*; D058949:Uromodulin
D001789:Blood Group Antigens; D009088:Mucoproteins; D010654:Phenylbutyrates; C548612:UMOD protein, human; D058949:Uromodulin; C075773:4-phenylbutyric acid; D003078:Colchicine