title: Production of human monoclonal antibody in eggs of chimeric chickens.
abstract: The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and downstream DNA sequences of ovalbumin, a protein produced exclusively in very high quantities in chicken egg white, to drive tissue-specific expression of human mAb in chicken eggs. To accommodate these large regulatory regions, we established and transfected lines of chicken embryonic stem (cES) cells and formed chimeras that express mAb from cES cell-derived tubular gland cells. Eggs from high-grade chimeras contained up to 3 mg of mAb that possesses enhanced antibody-dependent cellular cytotoxicity (ADCC), nonantigenic glycosylation, acceptable half-life, excellent antigen recognition and good rates of internalization.
authors.affiliation: Origen Therapeutics, 1450 Rollins Road, Burlingame, California 94010, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Marie-Cecile
authors.initials: MC
authors.lastname: van de Lavoir
authors.forename: Jenny
authors.initials: J
authors.lastname: Albanese
authors.forename: David O
authors.initials: DO
authors.lastname: Beenhouwer
authors.forename: Pina M
authors.initials: PM
authors.lastname: Cardarelli
authors.forename: Severino
authors.initials: S
authors.lastname: Cuison
authors.forename: David F
authors.initials: DF
authors.lastname: Deng
authors.forename: Shrikant
authors.initials: S
authors.lastname: Deshpande
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Diamond
authors.forename: Lynae
authors.initials: L
authors.lastname: Green
authors.forename: Edward L
authors.initials: EL
authors.lastname: Halk
authors.forename: Babette S
authors.initials: BS
authors.lastname: Heyer
authors.forename: Robert M
authors.initials: RM
authors.lastname: Kay
authors.forename: Allyn
authors.initials: A
authors.lastname: Kerchner
authors.forename: Philip A
authors.initials: PA
authors.lastname: Leighton
authors.forename: Christine M
authors.initials: CM
authors.lastname: Mather
authors.forename: Sherie L
authors.initials: SL
authors.lastname: Morrison
authors.forename: Zivko L
authors.initials: ZL
authors.lastname: Nikolov
authors.forename: David B
authors.initials: DB
authors.lastname: Passmore
authors.forename: Alicia
authors.initials: A
authors.lastname: Pradas-Monne
authors.forename: Benjamin T
authors.initials: BT
authors.lastname: Preston
authors.forename: Vangipuram S
authors.initials: VS
authors.lastname: Rangan
authors.forename: Mingxia
authors.initials: M
authors.lastname: Shi
authors.forename: Mohan
authors.initials: M
authors.lastname: Srinivasan
authors.forename: Steven G
authors.initials: SG
authors.lastname: White
authors.forename: Peggy
authors.initials: P
authors.lastname: Winters-Digiacinto
authors.forename: Susan
authors.initials: S
authors.lastname: Wong
authors.forename: Wen
authors.initials: W
authors.lastname: Zhou
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Etches
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D015139:Blotting, Southern; D015153:Blotting, Western; D016466:CHO Cells; D002152:Calorimetry, Differential Scanning; D002241:Carbohydrates / Q000737:chemistry; D002645:Chickens; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D004529:Egg White; D004622:Embryo, Mammalian / Q000166:cytology; D004625:Embryo, Nonmammalian; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005822:Genetic Vectors; D016678:Genome; D006031:Glycosylation; D006801:Humans; D007074:Immunoglobulin G; D007150:Immunohistochemistry; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C; D008957:Models, Genetic; D009005:Monosaccharides / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D010047:Ovalbumin / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D013234:Stem Cells / Q000166:cytology
chemical_list: D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D007074:Immunoglobulin G; D009005:Monosaccharides; D009844:Oligosaccharides; D011993:Recombinant Fusion Proteins; D010047:Ovalbumin; D004247:DNA