title: The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis.
abstract: Ca2+-regulated exocytosis of lysosomes was previously shown to be required for the repair of plasma membrane wounds. The small chemical vacuolin-1 alters the morphology of lysosomes without affecting the ability of cells to reseal their plasma membrane after injury. On the basis of a failure to detect Ca2+-triggered lysosomal exocytosis in vacuolin-1-treated cells, a recent study proposed that lysosomes are dispensable for resealing. Here, we show that vacuolin-1, despite altering lysosome morphology, does not inhibit the exocytosis of lysosomes induced by exposure to a Ca2+ ionophore, or by plasma membrane wounding. Thus, lysosomes cannot be excluded as agents of membrane repair in vacuolin-1-treated cells.
authors.affiliation: Section of Microbial Pathogenesis and Department of Cell Biology, Yale University School of Medicine, 295 Congress Avenue, New Haven, Connecticut 06536, USA.
authors.forename: Chau
authors.initials: C
authors.lastname: Huynh
authors.forename: Norma W
authors.initials: NW
authors.lastname: Andrews
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism* / Q000473:pathology; D018274:Electroporation; D005089:Exocytosis / Q000502:physiology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006576:Heterocyclic Compounds, 4 or More Rings / Q000378:metabolism*; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D051381:Rats; D001619:beta-N-Acetylhexosaminidases
chemical_list: D006576:Heterocyclic Compounds, 4 or More Rings; C503870:vacuolin-1; D001619:beta-N-Acetylhexosaminidases; D002118:Calcium