title: Solution-mediated effect of bioactive glass in poly (lactic-co-glycolic acid)-bioactive glass composites on osteogenesis of marrow stromal cells.
abstract: A previous study demonstrated that the incorporation of bioactive glass (BG) into poly (lactic-co-glycolic acid) (PLGA) can promote the osteoblastic differentiation of marrow stromal cells (MSCs) on PLGA by promoting the formation of a calcium-phosphate-rich layer on its surface. To further understand the mechanisms underlying the osteogenic effect of PLGA-BG composite scaffolds, whether solution-mediated factors derived from composite scaffolds/hybrids can promote osteogenesis of marrow stromal cells was tested. The dissolution product from PLGA-30%BG scaffold stimulated osteogenesis of MSCs, as was confirmed by increased mRNA expression of osteoblastic markers such as osteocalcin (OCN), alkaline phosphatase (ALP), and bone sialoprotein (BSP). The three-dimensional structure of the scaffolds may contribute to the production of cell-derived factors that promoted distant MSC differentiation. Thus PLGA-BG composites demonstrate significant potential as a bone-replacement material.
authors.affiliation: Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. junyao1@yahoo.com
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.forename: Shula
authors.initials: S
authors.lastname: Radin
authors.forename: Gwendolen
authors.initials: G
authors.lastname: Reilly
authors.forename: Phoebe S
authors.initials: PS
authors.lastname: Leboy
authors.forename: Paul
authors.initials: P
authors.lastname: Ducheyne
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000502:physiology*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D019344:Lactic Acid; D008297:Male; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology*; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017154:Stromal Cells / Q000502:physiology
chemical_list: D015415:Biomarkers; D011108:Polymers; D012333:RNA, Messenger; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000469:Alkaline Phosphatase