Subpallial origin of part of the calbindin-positive neurons of the claustral complex and piriform cortex.
The aim of the present study was to investigate whether part of the calbindin-positive neurons of the claustral complex and piriform cortex originate in the subpallium. To that end, we prepared organotypic cultures of embryonic telencephalic slices, and applied the cell tracker CMTMR to the ventricular/subventricular zone of the lateral or medial ganglionic eminence. Following 48 h of incubation, we observed a number of CMTMR-labeled cells (showing red fluorescence) of subpallial origin in the claustral complex and piriform cortex. To know whether some of these cells of subpallial origin were calbindin-positive, we performed immunofluorescence for calbindin using an Alexa 488-conjugated secondary antiserum (green fluorescence). Our results showed that some of the CMTMR-labeled cells of subpallial origin in the claustral complex and piriform cortex are calbindin-positive (and possibly GABAergic). The subpallial origin of part of these cells was confirmed by observation of double labeled neurons in the claustral complex that expressed both Lhx6 mRNA (a marker of cells derived from the medial ganglionic eminence) and calbindin. Future studies will be required to analyze the existence of a subpopulation of non-GABAergic calbindin cells in the claustral complex and piriform cortex, and to know their origin.
Legaz
Isabel
I
Department of Human Anatomy, Faculty of Medicine, University of Murcia, Spain.
García-López
Margarita
M
Medina
Loreta
L
D000818:Animals; D000831:Animals, Newborn; D002097:C-Reactive Protein / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D064026:Calbindins; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005917:Globus Pallidus / Q000166:cytology* / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D060850:LIM-Homeodomain Proteins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D012235:Rhodamines / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism; D000099153:Pentraxins
D002097:C-Reactive Protein; D015820:Cadherins; D064026:Calbindins; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D012235:Rhodamines; D064030:S100 Calcium Binding Protein G; D014157:Transcription Factors; D005680:gamma-Aminobutyric Acid; D000099153:Pentraxins; C116572:(((4-chloromethyl)benzoyl)amino)-tetramethylrhodamine; C112316:CDH8 protein, human; C498504:LHX6 protein, mouse