title: Induction of interleukin-6 and interleukin-8 gene expression by root canal sealers in human osteoblastic cells.
abstract: Histological investigations have demonstrated that root canal sealers can induce mild to severe inflammatory alternations. However, there is little information on the precise mechanisms about root canal sealers-induced inflammatory reaction. Dysregulated cytokine productions at local disease sites have been considered to be major contributors to the development of inflammatory diseases. Interleukin (IL)-6 and IL-8 released have been reported to play an important role in the pathogenesis of inflammation. The aim of this study was to investigate the effects of root canal sealers N2 (zinc-oxide eugenol based) and AH Plus (epoxy resin based) on the expression of IL-6 and IL-8 mRNA gene in human osteoblastic cell line U2OS cells. The levels of mRNAs were measured by the semi-quantitative reverse-transcriptase polymerase chain reaction analysis. The exposure of quiescent U2OS cells to N2 and AH Plus resulted in the induction of IL-6 and IL-8 mRNA gene expression (p < 0.05). The intensity of IL-8 mRNA gene was found to be significant higher than IL-6 mRNA gene (p < 0.05). Taken together, the activation of IL-6 and IL-8 mRNA gene expression may be one of the pathogenesis of zinc oxide-eugenol based and epoxy resin based root canal sealers-induced periapical inflammation.
authors.affiliation: Department of Dentistry, College of Oral Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Fu-Mei
authors.initials: FM
authors.lastname: Huang
authors.forename: Chung-Hung
authors.initials: CH
authors.lastname: Tsai
authors.forename: Shun-Fa
authors.initials: SF
authors.lastname: Yang
authors.forename: Yu-Chao
authors.initials: YC
authors.lastname: Chang
mesh_terms: D002478:Cells, Cultured; D004338:Drug Combinations; D004853:Epoxy Resins / Q000494:pharmacology*; D005054:Eugenol / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology*; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D015034:Zinc Oxide / Q000494:pharmacology*
chemical_list: D004338:Drug Combinations; D004853:Epoxy Resins; D015850:Interleukin-6; D016209:Interleukin-8; D012333:RNA, Messenger; D012387:Root Canal Filling Materials; C534916:epoxy resin-based root canal sealer; D005557:Formaldehyde; D005054:Eugenol; C003799:N2 Dental Cement; D015034:Zinc Oxide