title: [Relation between genotype of HBV and its clinical manifestation by PCR with genotype-specific primers].
abstract: OBJECTIVE
  To determine the relation between genotype of HBV and its clinical manifestation.
  
  
  METHODS
  Sera of 220 patients from Hunan Province of China, infected chronically with asymptomatic, mild, moderate, severe and fulminant hepatitis hepatitis B, were genotyped by PCR with genotype-specific primers. The results were statistically analyzed.
  
  
  RESULTS
  Only genotype B (86.4%) and C (13.6%) were found. With the aggravations of patients' condition, genotype C was found more often (P < 0.05). The levels of alanine aminotransferase (ALT), total bilirubin (TBIL) and HBV-DNA of genotype C was higher than those of genotype B, but there was no statistical difference. However, the ALT elevation rates of genotype C (96.7%) were significantly higher than those of genotype B (75.2%) (P < 0.05). There was no statistical difference in HBeAg rate between genotype B and C in general, but in patients with chronic fulminant hepatitis or aged 21 approximately 30, the HBeAg rates of genotype C (35.0% and 50.0%) were significantly higher than those of genotype B (14.4% and 24.5%) (P < 0.05).
  
  
  CONCLUSIONS
  The dominant genotype of HBV in Hunan Province of China is genotype B and C. Genotype of HBV correlates with its clinical manifestation and genotype C of HBV can lead to more severe hepatitis than genotype B of HBV.
authors.affiliation: Department of Epidemiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Zhi-Li
authors.initials: ZL
authors.lastname: Wen
authors.forename: De-Ming
authors.initials: DM
authors.lastname: Tan
authors.forename: Zhou-Hua
authors.initials: ZH
authors.lastname: Hou
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D017114:Liver Failure, Acute / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods