Chitinase production in solid-state fermentation by Enterobacter sp. NRG4 using statistical experimental design.
The optimization of nutrient levels for chitinase production by Enterobacter sp. NRG4 in solid-state fermentation conditions (SSF) was carried out using response surface methodology (RSM) based on central composite design (CCD). The design was employed by selecting wheat bran-to-flake chitin ratio, moisture level, inoculum size, and incubation time as model factors. The results of first-order factorial design experiments showed that all four independent variables have significant effects on chitinase production. The optimum concentrations for chitinase production were wheat bran-to-flake chitin ratio, 1; moisture level, 80%; inoculum size, 2.6 mL; and incubation time, 168 h. Using this statistical optimization method, chitinase production was found to increase from 616 U . g(-1) dry weight of solid substrate to 1475 U . g(-1) dry weight of solid substrate.
Dahiya
Neetu
N
Department of Biotechnology, Panjab University, Chandigarh, India. ineetudahiya@yahoo.com
Tewari
Rupinder
R
Tiwari
Ram Prakash
RP
Hoondal
Gurinder Singh
GS
D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D004754:Enterobacter / Q000145:classification / Q000201:enzymology* / Q000378:metabolism; D005285:Fermentation; D012107:Research Design; D013379:Substrate Specificity; D013696:Temperature
D002686:Chitin; D002688:Chitinases