title: Analysis of phenoxymethylpenicillin potassium by capillary electrophoresis.
abstract: The application of capillary electrophoresis for separation of penicillin V and its impurities was investigated. The phosphate-borate buffer supplemented with sodium dodecyl sulfate (SDS) 20.0 g/L (69 mM) and pentanesulfonic acid sodium salt (PS) 2.2 g/L (12.5 mM) adjusted to pH 6.3, and current voltage 15 kV seem to provide optimal conditions for this aim. The resolution between penicillin V and each impurity was very good. The statistical analysis of phenoxymethylpenicillin V assay showed no significant differences between the results obtained by CE and HPLC methods.
authors.affiliation: Department of Antibiotics and Microbiology, National Institute of Public Health, 30/34 Chebmska Street, 00-725 Warsaw, Poland. tyski@il.waw.pl
authors.forename: Genowefa
authors.initials: G
authors.lastname: Pajchel
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Michalska
authors.forename: Stefan
authors.initials: S
authors.lastname: Tyski
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D010404:Penicillin V / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D010404:Penicillin V