Validation of liquid chromatographic method for analysis of lidocaine hydrochloride, dexamethasone acetate, calcium dobesilate, buthylhydroxyanisol and degradation product hydroquinone in suppositories and ointment.
In this paper, there was developed a sensitive, precise and accurate reversed-phase liquid chromatographic (RP-HPLC) method and validated for simultaneous determination of lidocaine hydrochloride, dexamethasone acetate (DA) and calcium dobesilate (CD) in suppositories and ointment. Also there was achieved a parallel analysis of buthylhydroxyanisol, as a preservative, and hydroquinone, as a degradation product of calcium dobesilate, present in these dosage forms. The relative standard deviation (RSD) values for all five compounds indicated a good precision and accuracy of the RP-HPLC method. Method is selective, sensitive and reproducible with good recovery values and can be applied in simultaneous determination of all mentioned compounds.
Zivanovic
Lj
Lj
Department of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, Vojvode Stepe 450, Belgrade, Serbia. ljzivan@pharmacy.bg.ac.yu
Zecevic
M
M
Markovic
S
S
Petrovic
S
S
Ivanovic
I
I
D002083:Butylated Hydroxyanisole / Q000032:analysis*; D002123:Calcium Dobesilate / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000032:analysis; D006873:Hydroquinones / Q000032:analysis*; D008012:Lidocaine / Q000032:analysis*; D009824:Ointments / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013488:Suppositories / Q000737:chemistry*
D006873:Hydroquinones; D009824:Ointments; D013488:Suppositories; D002083:Butylated Hydroxyanisole; D002123:Calcium Dobesilate; D003907:Dexamethasone; D008012:Lidocaine; C018038:dexamethasone acetate; C031927:hydroquinone