title: PCR detection of viral DNA in serum as an ancillary analysis for the diagnosis of acute mononucleosis-like syndrome due to human cytomegalovirus (HCMV) in immunocompetent patients.
abstract: BACKGROUND
  Serologic tests are occasionally inconclusive for the diagnosis of mononucleosis-like syndrome due to human cytomegalovirus (HCMV).
  
  
  OBJECTIVE
  To determine the value of viral DNA detection in serum by PCR as an ancillary test for the diagnosis of HCMV mononucleosis.
  
  
  METHODS
  Sera from 34 previously healthy individuals with HCMV mononucleosis, obtained within 1 month after the onset of symptoms, were assayed for viral DNA by three commercial PCRs (QCA gB, QCA MIE and Amplicor CMV Monitor). Sera from 30 patients with evidence of past HCMV infection served as controls.
  
  
  RESULTS
  Viral DNA was detected in 20% of the samples from patients with HCMV mononucleosis by both QCA procedures, but in none of the controls. All samples tested negative by the Amplicor CMV Monitor.
  
  
  CONCLUSIONS
  Analysis of sera for the presence of HCMV DNA is of limited value for the diagnosis of HCMV mononucleosis.
authors.affiliation: Department of Microbiology, University Clinic Hospital, Blasco Ibáñez 17, 46010 Valencia, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Navalpotro
authors.forename: Concepción
authors.initials: C
authors.lastname: Gimeno
authors.forename: David
authors.initials: D
authors.lastname: Navarro
mesh_terms: D000293:Adolescent; D000914:Antibodies, Viral; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000097:blood / Q000276:immunology* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis* / Q000097:blood; D005260:Female; D006801:Humans; D007244:Infectious Mononucleosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D008297:Male; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic