title: Phospholipase Cdelta1 associates with importin beta1 and translocates into the nucleus in a Ca2+-dependent manner.
abstract: Phospholipase C (PLC)delta1 shuttles between the nucleus and the cytoplasm. Here, we demonstrate that treatment of MDCK cells and PC12 cells with ionomycin causes nuclear accumulation of ectopically expressed and endogenous PLCdelta1, respectively, suggesting that signals that increase [Ca2+]i trigger nuclear translocation. To clarify the molecular mechanisms involved in this translocation, we have examined whether PLCdelta1 binds with importins. PLCdelta1 interacted with importin beta1 in a Ca2+-dependent manner in vitro even in the absence of importin alpha. A PLCdelta1 mutant E341A, which lacks Ca2+-binding to the catalytic core, did not show this interaction at any physiological Ca2+ concentration and did not translocate into the nucleus after ionomycin treatment when expressed in MDCK cells. These results suggested that the nuclear import of PLCdelta1 is mediated by its Ca2+-dependent interaction with importin beta1.
authors.affiliation: Graduate School of Life Science, University of Hyogo, Harima Science Garden City, Hyogo 678-1297, Japan. masashi@stkt.u-hyogo.ac.jp
authors.forename: Masashi
authors.initials: M
authors.lastname: Okada
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ishimoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Naito
authors.forename: Hajime
authors.initials: H
authors.lastname: Hirata
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yagisawa
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004285:Dogs; D015759:Ionomycin / Q000378:metabolism; D007476:Ionophores / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D054803:Phospholipase C delta; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D010738:Type C Phospholipases / Q000235:genetics / Q000378:metabolism*; D028961:beta Karyopherins / Q000235:genetics / Q000378:metabolism*
chemical_list: D007476:Ionophores; D007527:Isoenzymes; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D028961:beta Karyopherins; D015759:Ionomycin; D010738:Type C Phospholipases; D054803:Phospholipase C delta; C518026:Plcd1 protein, rat; D002118:Calcium