title: Monocyte enriched apheresis for preparation of dendritic cells (DC) to be used in cellular therapy.
abstract: We describe that with one leukapheresis procedure it is feasible to obtain sufficient numbers of monocytes to be utilized in dendritic cell therapies. Twenty-two leukaphereses were performed on eight healthy volunteers and 13 cancer patients, using Cobe Spectra. An on-line sample was drawn as soon as a stable interface was established. The concentration of monocytes in the sample was used to calculate the volume to be collected to reach target numbers of monocytes. A recovery unit was used to calculate the efficacy of the leukaphereses and we demonstrate an efficacy for monocytes correlating with the amount of processed blood.
authors.affiliation: Immunhemotherapy unit, Bldg L2:00, Department of Clinical Immunology and Transfusion Medicine, Karolinska University Hospital and Karolinska Institute, SE-171 76 Stockholm, Sweden. anna.svensson@karolinska.se
authors.forename: Anna
authors.initials: A
authors.lastname: Svensson
authors.forename: Lars
authors.initials: L
authors.lastname: Adamson
authors.forename: Pavel
authors.initials: P
authors.lastname: Pisa
authors.forename: Max
authors.initials: M
authors.lastname: Petersson
authors.forename: Mona
authors.initials: M
authors.lastname: Hansson
mesh_terms: D000328:Adult; D000368:Aged; D003713:Dendritic Cells / Q000166:cytology / Q000637:transplantation; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods; D007937:Leukapheresis / Q000379:methods; D007958:Leukocyte Count / Q000379:methods; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology; D009369:Neoplasms / Q000628:therapy*