title: [Preparation of transfersomes of vincristine sulfate and study on its prcutaneous penetration].
abstract: OBJECTIVE
  To select the best preparation method of vincristine transfersomes (VCR-T) and predict its possibility of being a new formulation of VCR.
  
  
  METHODS
  Orthogonal design was used to optimize the preparation methods on the basis of single factor pretests; and the permeation tests in vitro were performed in modified Franz diffusion cells.
  
  
  RESULTS
  The optimum formula was: pH was equal to 7.3, the ratio of lecithin to sodium deoxycholate is 70/20, the weight of VCR is 10 mg, hydrating time is 30 minutes. The optimized solution was light yellow and transparent colloid solution. The VCR-T are spherical and smooth with average diameters of 94 nm and an encapsulation ratio of 90%. The test in vitro showed that VCR-T could permeat through mouse skin at zero rate with the cumulative penetrating quality amounting to 63.8%.
  
  
  CONCLUSIONS
  Transfersomes may become a promising carrier of VCR for clinic use.
authors.affiliation: College of Pharmacy, Sichuan University, Chengi 610041, China. toluyi@163.com
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Shi-Xiang
authors.initials: SX
authors.lastname: Hou
authors.forename: Tong
authors.initials: T
authors.lastname: Chen
authors.forename: Yi-Yi
authors.initials: YY
authors.lastname: Sun
authors.forename: Ben-Xia
authors.initials: BX
authors.lastname: Yang
authors.forename: Zi-Yan
authors.initials: ZY
authors.lastname: Yuan
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000493:pharmacokinetics; D003840:Deoxycholic Acid; D004337:Drug Carriers; D006863:Hydrogen-Ion Concentration; D051379:Mice; D010316:Particle Size; D010713:Phosphatidylcholines; D012869:Skin Absorption; D013678:Technology, Pharmaceutical / Q000379:methods*; D014750:Vincristine / Q000008:administration & dosage* / Q000493:pharmacokinetics
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004337:Drug Carriers; D010713:Phosphatidylcholines; D003840:Deoxycholic Acid; D014750:Vincristine