Structural and functional characterization of two novel peptide toxins isolated from the venom of the social wasp Polybia paulista.
Two novel inflammatory peptides were isolated from the venom of the social wasp Polybia paulista. They had their molecular masses determined by ESI-MS and their primary sequences were elucidated by Edman degradation chemistry as: Polybia-MPI: I D W K K L L D A A K Q I L-NH2 (1654.09 Da), Polybia-CP: I L G T I L G L L K S L-NH2 (1239.73 Da). Both peptides were functionally characterized by using Wistar rat cells. Polybia-MPI is a mast cell lytic peptide, which causes no hemolysis to rat erythrocytes and presents chemotaxis for polymorphonucleated leukocytes (PMNL) and with potent antimicrobial action both against Gram-positive and Gram-negative bacteria. Polybia-CP was characterized as a chemotactic peptide for PMNL cells, presenting antimicrobial action against Gram-positive bacteria, but causing no hemolysis to rat erythrocytes and no mast cell degranulation activity at physiological concentrations.
Souza
Bibiana M
BM
CEIS, Department of Biology/IBRC-UNESP (CAT-CEPID/FAPESP), Institute of Immunological Investigations (Millennium Institute-MCT/CNPq), Rio Claro, SP 13506-900, Brazil.
Mendes
Maria A
MA
Santos
Lucilene D
LD
Marques
Maurício R
MR
César
Lilian M M
LM
Almeida
Roberta N A
RN
Pagnocca
Fernando C
FC
Konno
Katsuhiro
K
Palma
Mario S
MS
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000633:toxicity; D015550:Cell Degranulation / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects / Q000502:physiology; D007962:Leukocytes / Q000166:cytology / Q000502:physiology; D008407:Mast Cells / Q000166:cytology / Q000502:physiology; D010455:Peptides / Q000737:chemistry* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D014862:Wasp Venoms / Q000737:chemistry* / Q000633:toxicity; D014863:Wasps / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D010455:Peptides; D014862:Wasp Venoms