title: Selective immobilization of fusion proteins on poly(hydroxyalkanoate) microbeads.
abstract: A novel fusion protein system employing the substrate-binding domain (SBD) of poly(hydroxyalkanoate) (PHA) depolymerase was developed for the specific immobilization of proteins on PHA microbeads, and was consequently used for immunoassays. The enhanced green fluorescent protein, red fluorescent protein, and severe acute respiratory syndrome coronavirus envelope protein were used as model proteins, and were selectively and functionally immobilized to the PHA microbeads by fusing them to the SBD. Using this PHA microbead system combined with SBD fusion technology, immunoassays could be successfully carried out.
authors.affiliation: Department of Chemical & Biomolecular Engineering, BioProcess Engineering Research Center, Daejeon 305-701, Korea.
authors.forename: Seok Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jong Pil
authors.initials: JP
authors.lastname: Park
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Park
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Seongnam
authors.initials: S
authors.lastname: Lee
authors.forename: Jung Ki
authors.initials: JK
authors.lastname: Park
mesh_terms: D005434:Flow Cytometry; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011091:Polyesters / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D011091:Polyesters; D011993:Recombinant Fusion Proteins