Acetazolamide affects breathing differently in ICR and C57 mice.
Acetazolamide (ACZ) administration was compared on ventilation in outbred male ICR Swiss Webster (ICR) and inbred C57BL/6J (C57) mice, used in development of transgenic strains. We hypothesized that in both strains ACZ would affect breathing similarly. Mice received intraperitoneally vehicle and the next week ACZ (40 mg/kg), and were exposed to air for 90 min, followed by 5-min exposure to 10% O(2), air for 15 min, and to 5 min of 5% CO(2) in O(2). Ventilation was evaluated using plethysmography. ACZ stimulated ventilation in both stains exposed to air. C57 mice minimally increased frequency and tidal volume, whereas ICR mice markedly increased frequency. Strain differences in the ventilatory pattern in response to hypoxia and hypercapnia occurred. ACZ-treated ICR mice decreased hypoxic responsiveness to 50% of vehicle values, whereas ACZ had no effect in C57 mice. ACZ decreased hypercapnic ventilatory responsiveness in both strains. Differential effects of ACZ breathing in these two strains suggest that genetic factors modulate its effect on breathing.
Schlenker
Evelyn
E
Division of Basic Biomedical Sciences, University of South Dakota School of Medicine, Vermillion, 57069, USA. eschlenk@usd.edu
Shi
Yijiang
Y
Johnson
Chris
C
Wipf
Joni
J
D000086:Acetazolamide / Q000494:pharmacology*; D000818:Animals; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D012123:Pulmonary Ventilation / Q000187:drug effects; D012119:Respiration / Q000187:drug effects*; D013045:Species Specificity; D013990:Tidal Volume / Q000187:drug effects
D002257:Carbonic Anhydrase Inhibitors; D000086:Acetazolamide