title: [Determination of platelet serotonin by high performance liquid chromatography with fluorometric detector].
abstract: OBJECTIVE
  To establish a simple, rapid and precise method to determine platelet serotonin.
  
  
  METHODS
  Serotonin (5-hydroxytryptamine; 5-HT) was determined in platelet pellet after simple deproteinization, following ion-pair high performance liquid chromatography (HPLC) on reverse-phase column: Nova-pak C18 column (150 mm x 4.6 mm, 4 microm), serotonin and internal standard (N-methylserotonin) were detected by fluorometry.
  
  
  RESULTS
  The intra-day CV was 2.29% and inter-day CV was 3.88%. The mean recovery of serotonin in platelet was 97.5%, the linear range was 20 - 20000 nmol/ L, and the detection limit was 1 nmol/L. 5-hydroxyindole acetic acid (5-HIAA), Trp, Phe and Tyr had no interference with the chromatographic condition used in this trial.
  
  
  CONCLUSIONS
  The metod described here is simple, convenient, rapid, sensitive, and accurate for clinical and scientific research.
authors.affiliation: Department of Laboratory Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Tang
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Tan
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005470:Fluorometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000097:blood*
chemical_list: D012701:Serotonin