Establishment and characterization of a human colonic mucinous carcinoma cell line with predominant goblet-cell differentiation from liver metastasis.
Many human colorectal adenocarcinoma cell lines have been developed. However, differentiated type colorectal cancer cell lines, particularly, the goblet-cell differentiated type, are scarce. In the present study a novel colorectal adenocarcinoma cell line (designated as COLM-6) with predominant goblet-cell differentiation was established from the rectal mucinous adenocarcinoma of a Japanese woman. COLM-6 cells grow in a typical epithelial monolayer in culture. They expressed epidermal growth factor (EGF) receptor and HER2 on their surface and accordingly, their growth was significantly stimulated by EGF, transforming growth factor (TGF)-alpha and heregulin. COLM-6 cells form tumor with typical mucinous adenocarcinomatous appearance in nude mice. Immunohistochemical analysis of these subcutaneous tumors demonstrated that COLM-6 cells strongly express MUC2 as a goblet-cell marker and Cdx2 in the nucleus. Some weakly express villin and carbonic anhydrase 1 as a columnar absorptive-cell marker as well. They were also positive for adenomatous polyposis coli (APC) cytoplasmically and expressed beta-catenin in their cytoplasm and cell membrane without nuclear accumulation. These results indicate that COLM-6 cell line has unique characteristics and may provide a useful tool to study the mechanism of growth and differentiation of colonic epithelium as well as the biological behavior of colorectal mucinous adenocarcinoma.
Yamachika
Takasuke
T
Division of Oncological Pathology, Aichi Cancer Center Research Institute, Nagoya, Japan. tyamach@se.starcat.ne.jp
Nakanishi
Hayao
H
Yasui
Kenzo
K
Ikehara
Yuzuru
Y
Niwa
Toru
T
Wanibuchi
Hideki
H
Tatematsu
Masae
M
Fukushima
Shoji
S
D002288:Adenocarcinoma, Mucinous / Q000378:metabolism / Q000556:secondary* / Q000601:surgery; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D020397:Goblet Cells / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D013047:Specific Pathogen-Free Organisms
D014408:Biomarkers, Tumor