Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454).
A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP.
Sekiguchi
Jun-ichiro
J
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan.
Tharavichitkul
Prasit
P
Miyoshi-Akiyama
Tohru
T
Chupia
Vena
V
Fujino
Tomoko
T
Araake
Minako
M
Irie
Atsushi
A
Morita
Koji
K
Kuratsuji
Tadatoshi
T
Kirikae
Teruo
T
D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000494:pharmacology*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003428:Cross Infection / Q000382:microbiology*; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005493:Folic Acid Antagonists / Q000494:pharmacology*; D006801:Humans; D016106:Methicillin Resistance; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D014295:Trimethoprim / Q000494:pharmacology*
D000890:Anti-Infective Agents; D003470:Culture Media; D005493:Folic Acid Antagonists; D014295:Trimethoprim; D013762:Tetrahydrofolate Dehydrogenase