Quantitative analysis of pyoluteorin in anti-fungal fermentation liquor of Pseudomonas species by capillary zone electrophoresis with UV-vis detector.
This paper investigated potential utility of capillary zone electrophoresis (CZE) for very succinct but robust quantitative analysis of pyoluteorin (Plt) in anti-fungal fermentation liquor of Pseudomonas species. The experimental conditions for the separation and quantification of Plt were optimized at first. The optimized conditions are: 80 mmol/L pH 8.40 Gly-NaOH buffer, 51 cm total length (42 cm effective) and 75 microm I.D. capillary, 230 nm wavelength, 25 kV, 13 mbar 10s pressure sample injection and 24 degrees C air-cooling. Under the optimized conditions, the migration times of Plt and the internal standard phenobarbital are 2.09 and 2.49 min, respectively, the linear response of Plt concentration ranges from 5.0 to 1000 microg/mL with high correlation coefficient (r=0.99977, n=9), the limits of detection (LOD) and quantification (LOQ) for Plt are 0.66 and 2.2 microg/mL, the precision values (expressed as R.S.D.) of intra- and inter-day are 1.19-1.94% and 1.55-6.21%, respectively, the recoveries of Plt at three concentration levels of 750, 250 and 50 microg/mL range from 90.31% to 97.85% and to 98.96%, respectively. The developed method can be well used for the quantification of Plt in the fermentation liquor.
Wang
Qiu-Ling
QL
Laboratory of Analytical Biochemistry and Bioseparation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd. Minhang, 200240 Shanghai, PR China.
Zhang
Xue-Hong
XH
Fan
Liu-Yin
LY
Zhang
Wei
W
Xu
Yu-Qian
YQ
Hu
Hong-Bo
HB
Cao
Cheng-Xi
CX
D000935:Antifungal Agents / Q000032:analysis* / Q000302:isolation & purification; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification; D011549:Pseudomonas / Q000378:metabolism*; D011758:Pyrroles / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; D002021:Buffers; D010636:Phenols; D011758:Pyrroles; C095503:pyoluteorin