title: Binding of all-trans-retinoic acid to MLTC-1 proteins.
abstract: The covalent incorporation of [(3)H]all-trans-retinoic acid into proteins has been studied in tumoural Leydig (MLTC-1) cells. The maximum retinoylation activity of MLTC-1 cell proteins was 710+/-29 mean+/-SD) fmoles/8 x 10(4) cells at 37 degrees C. About 90% of [(3)H]retinoic acid was trichloroacetic acid-soluble after proteinase-K digestion and about 65--75% after hydrolysis with hydroxylamine. Thus, retinoic acid is most probably linked to proteins as a thiol ester. The retinoylation reaction was inhibited by 13-cis-retinoic acid and 9-cis-retinoic acid with IC(50) values of 0.9 microM and 0.65 microM, respectively. Retinoylation was not inhibited by high concentrations of palmitic or myristic acids (250 microM); but there was an increase of the binding activity of about 25% and 130%, respectively. On the other hand, the retinoylation reaction was inhibited (about 40%) by 250 microM lauric acid. After pre-incubation of the cells with different concentrations of unlabeled RA, the retinoylation reaction with 100 nM [(3)H]RA involved first an increase at 100 nM RA and then a decrease of retinoylation activity between 200 and 600 nM RA. After cycloheximide treatment of the tumoural Leydig cells the binding activity of [(3)H]RA was about the same as that in the control, suggesting that the bond occurred on proteins in pre-existing cells.
authors.affiliation: Dipartimento Farmaco-Biologico, Università della Calabria, Cosenza 87100, Italia.
authors.forename: Erika
authors.initials: E
authors.lastname: Cione
authors.forename: Paola
authors.initials: P
authors.lastname: Tucci
authors.forename: Valentina
authors.initials: V
authors.lastname: Senatore
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Ioele
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Genchi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D007700:Kinetics; D007985:Leydig Cells / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000378:metabolism*; D011485:Protein Binding; D014212:Tretinoin / Q000378:metabolism*
chemical_list: D009363:Neoplasm Proteins; D014212:Tretinoin