Expression analysis of immune-related genes in CD34(+) progenitor-derived dendritic cells after exposure to the chemical contact allergen DNCB.
We studied the changes in gene expression after exposure of human dendritic cells (DCs) to the model allergen dinitrochlorobenzene (DNCB). DCs were derived from CD34(+) progenitor cells of three different donors and exposed to 10 microM DNCB or solvent for several time intervals (3, 6 and 12h). cDNA microarrays were used to assess the transcriptional activity of 11,000 human genes. Compared to control gene expression, changes larger than +/-two-fold were observed for 241 genes after exposure to DNCB. Of these genes, 137 were up-regulated and 104 down-regulated. Twenty of these genes encode proteins that are related to the immune response (cytokines, chemokines, their receptors, cytokine/chemokines-related genes, transcription and signal transduction genes) and are discussed in more detail. Our data indicate that exposure to DNCB does not induce a typical maturation pattern in DCs.
Schoeters
E
E
Vito (Flemish Institute for Technological Research), Centre of Expertise in Environmental Toxicology, Boeretang 200, 2400 Mol, Belgium.
Verheyen
G R
GR
Van Den Heuvel
R
R
Nelissen
I
I
Witters
H
H
Van Tendeloo
V F I
VF
Schoeters
G E R
GE
Berneman
Z N
ZN
D000485:Allergens / Q000633:toxicity*; D018952:Antigens, CD34 / Q000032:analysis / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D018925:Chemokines / Q000096:biosynthesis / Q000235:genetics; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D004137:Dinitrochlorobenzene / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000235:genetics; D013997:Time Factors
D000485:Allergens; D018952:Antigens, CD34; D018925:Chemokines; D016207:Cytokines; D004137:Dinitrochlorobenzene; D019707:Receptors, Chemokine