title: [Construction of co-expression vector pSLC-IRES-IL-2 and its expression in COS-7 cells].
abstract: OBJECTIVE
  To construct an eukaryotic co-expression plasmid pSLC-IRES-IL-2, and to express it in COS-7 cells.
  
  
  METHODS
  Human IL-2 and SLC genes were cloned by RT-PCR and PCR, respectively, and then the eukaryotic expression plasmid pSLC-IRES-IL-2 was constructed.The constructed plasmid was transfected into COS-7 cells by electroporation method. The expression of SLC and IL-2 was detected by Western blot.
  
  
  RESULTS
  SLC and IL-2 in the culture supernatant and lysate of transfected COS-7 cells were detected by Western blot. The relative molecular masses of the expressed products were consistent with the theoretical values.
  
  
  CONCLUSIONS
  The SLC and IL-2 co-expression plasmid is successfully constructed and expressed in COS-7 cells, which offers a pathway for research on gene therapy of tumors.
authors.affiliation: Medical Research Center of Shandong Provincial Hospital, Shandong University, Jinan 250021, China. angelhl@126.com
authors.forename: Li
authors.initials: L
authors.lastname: Hou
authors.forename: Yue-ran
authors.initials: YR
authors.lastname: Zhao
authors.forename: Lai-cheng
authors.initials: LC
authors.lastname: Wang
authors.forename: Yu-lian
authors.initials: YL
authors.lastname: Jiao
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Ma
authors.forename: Bin
authors.initials: B
authors.lastname: Cui
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D054421:Chemokine CCL21 / Q000235:genetics* / Q000378:metabolism*; D002522:Chlorocebus aethiops; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D054421:Chemokine CCL21; D007376:Interleukin-2