Quantitative determination of SH groups using 19F NMR spectroscopy and disulfide of 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid.
A new method of measurement of thiol concentration by 19F NMR spectroscopy is developed. The method is based on the detection of products of the exchange reaction of thiols with a newly synthesized fluorinated disulfide, 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid (BSSB). A significant broadening of the 19F NMR signal of BSSB in the presence of thiols was observed and attributed to the exchange reaction between the parent disulfide and 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid. The rate constant for this reaction was found to be equal to (63 +/- 11) x 10(3) M(-1) s(-1) at pH 7.0. The method was applied for the measurement of concentration of glutathione and albumin in rat blood.
Potapenko
Dmitrii I
DI
International Tomography Center, Novosibirsk 630090, Russia.
Bagryanskaya
Elena G
EG
Grigoriev
Igor A
IA
Maksimov
Aleksander M
AM
Reznikov
Vladimir A
VA
Platonov
Vyacheslav E
VE
Clanton
Thomas L
TL
Khramtsov
Valery V
VV
D000818:Animals; D001565:Benzoates / Q000737:chemistry*; D004220:Disulfides / Q000097:blood / Q000737:chemistry*; D005461:Fluorine; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards; D008297:Male; D015394:Molecular Structure; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D013438:Sulfhydryl Compounds / Q000097:blood / Q000737:chemistry*
C507891:2,3,5,6-tetrafluoro-4-mercaptobenzoic acid disulfide; C507890:2,3,5,6-tetrafluoro-4-sulfanylbenzoic acid; D001565:Benzoates; D004220:Disulfides; D013438:Sulfhydryl Compounds; D005461:Fluorine