Carbonic anhydrase-related protein VIII deficiency is associated with a distinctive lifelong gait disorder in waddles mice.
The waddles (wdl) mouse is a unique animal model that exhibits ataxia and appendicular dystonia without pathological abnormalities of either the central or the peripheral nervous systems. A 19-bp deletion in exon 8 of the carbonic anhydrase-related protein VIII gene (Car8) was detected by high-throughput temperature-gradient capillary electrophoresis heteroduplex analysis of PCR amplicons of genes and ESTs within the wdl locus on mouse chromosome 4. Although regarded as a member of the carbonic anhydrase gene family, the encoded protein (CAR8) has no reported enzymatic activity. In normal mice, CAR8 is abundantly expressed in cerebellar Purkinje cells as well as in several other cell groups. Compatible with nonsense-mediated decay of mutant transcripts, CAR8 is virtually absent in mice homozygous for the wdl mutation. These data indicate that the wdl mouse is a Car8 null mutant and that CAR8 plays a central role in motor control.
Jiao
Yan
Y
Department of Orthopedic Surgery-Campbell Clinic and Pathology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA.
Yan
Jian
J
Zhao
Yu
Y
Donahue
Leah Rae
LR
Beamer
Wesley G
WG
Li
Xinmin
X
Roe
Bruce A
BA
Ledoux
Mark S
MS
Gu
Weikuan
W
D000595:Amino Acid Sequence; D000818:Animals; D014408:Biomarkers, Tumor; D002256:Carbonic Anhydrases / Q000172:deficiency* / Q000235:genetics; D002874:Chromosome Mapping; D005808:Genes, Recessive; D007150:Immunohistochemistry; D007794:Lameness, Animal / Q000235:genetics*; D051379:Mice; D008818:Mice, Neurologic Mutants; D008969:Molecular Sequence Data; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000172:deficiency* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
D014408:Biomarkers, Tumor; C512821:Car8 protein, mouse; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D002256:Carbonic Anhydrases