Study of the inhibition of alpha-amylase by the benzo[c]phenanthridine alkaloids sanguinarine and chelerythrine.
Inhibition of porcine pancreas and human saliva alpha-amylase (EC 3.2.1.1) by sanguinarine and chelerythrine was studied. The inhibition of alpha-amylase was assayed using a biosensor method which utilises a flow system equipped with a peroxide electrode. 250 microM sanguinarine and 250 microM chelerythrine cause complete inhibition of 1.9 nkat alpha-amylase from porcine pancreas. The same concentration of sanguinarine and chelerythrine caused 23.9% and 7.5% inhibition, respectively, of 1.9 nkat alpha-amylase from human saliva. Mixed type and partially reversible inhibition was found for both alpha-amylases treated with either alkaloid.
Zajoncova
Ludmila
L
Department of Biochemistry, Faculty of Science, Palacký University, Slechtitelů 11, 783 71, Olomouc, Czech Republic. zajoncov@prfholnt.upol.cz
Kosina
Pavel
P
Vicar
Jaroslav
J
Ulrichová
Jitka
J
Pec
Pavel
P
D000470:Alkaloids / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D053119:Benzophenanthridines; D015374:Biosensing Techniques; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D006801:Humans; D007546:Isoquinolines; D007700:Kinetics; D010617:Phenanthridines / Q000378:metabolism* / Q000494:pharmacology; D013552:Swine; D013997:Time Factors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism
D000470:Alkaloids; D053119:Benzophenanthridines; D004791:Enzyme Inhibitors; D007546:Isoquinolines; D010617:Phenanthridines; C005705:sanguinarine; C016299:chelerythrine; D000516:alpha-Amylases