Role of toll-like receptor 4 in the proinflammatory response to Vibrio cholerae O1 El tor strains deficient in production of cholera toxin and accessory toxins.
Following intranasal inoculation, Vibrio cholerae KFV101 (DeltactxAB DeltahapA DeltahlyA DeltartxA) colonizes and stimulates tumor necrosis factor alpha and interleukin 1beta (IL-1beta) in mice, similar to what occurs with isogenic strain P4 (DeltactxAB), but is less virulent and stimulates reduced levels of IL-6, demonstrating a role for accessory toxins in pathogenesis. Morbidity is enhanced in C3H/HeJ mice, indicating that Toll-like receptor 4 is important for infection containment.
Haines
G Kenneth
GK
Northwestern University, Dept. of Microbiology-Immunology, 303 E. Chicago Avenue, Tarry 3-713, Chicago, IL 60611, USA.
Sayed
Blayne Amir
BA
Rohrer
Melissa S
MS
Olivier
Verena
V
Satchell
Karla J Fullner
KJ
D000818:Animals; D002771:Cholera / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D002772:Cholera Toxin / Q000096:biosynthesis* / Q000235:genetics*; D022121:Cholera Vaccines / Q000276:immunology; D004195:Disease Models, Animal; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D007375:Interleukin-1 / Q000096:biosynthesis; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D011650:Pulmonary Alveoli / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D011971:Receptors, Immunologic / Q000502:physiology*; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014734:Vibrio cholerae / Q000276:immunology / Q000378:metabolism*
D022121:Cholera Vaccines; D007375:Interleukin-1; D011971:Receptors, Immunologic; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D002772:Cholera Toxin