title: Angiogenesis in the human corpus luteum: changes in expression of angiopoietins in the corpus luteum throughout the menstrual cycle and in early pregnancy.
abstract: BACKGROUND
  Blood vessel stabilization is regulated by angiopoietins and important for angiogenesis in the corpus luteum.
  
  
  OBJECTIVE
  To study angiogenesis and blood vessel stabilization in the human corpus luteum, changes in expression of angiopoietin (Ang)-1, Ang-2, and their specific receptor, Tie-2, together with the number of blood vessels and pericytes were examined in the corpus luteum throughout the menstrual cycle and in early pregnancy.
  
  
  METHODS
  The number of blood vessels and pericytes was determined by immunohistochemistry for CD34 and alpha-smooth muscle actin, respectively. Ang and Tie-2 expression were examined by immunohistochemistry or RT-PCR.
  
  
  RESULTS
  The number of blood vessels increased during the early luteal phase, whereas the number of pericytes was small in the early luteal phase and increased in the midluteal phase, suggesting that angiogenesis is undergoing during the early luteal phase and blood vessels are stabilized in the midluteal phase. Blood vessels and pericytes decreased in number during the late luteal phase. The increased number of both blood vessels and pericytes seen in the corpus luteum of early pregnancy suggests that angiogenesis is undergoing accompanied by blood vessel stabilization. Ang-2 expression with low Ang-1 expression was found during the early luteal phase. Thereafter, increasing Ang-1 expression during the midluteal phase, declining Ang-1 expression with continued Ang-2 expression during the late luteal phase, and relatively high Ang-1 expression in early pregnancy were observed.
  
  
  CONCLUSIONS
  The change in Ang expression is closely associated with angiogenesis, blood vessel stabilization, and blood vessel regression during the divergent phases of luteal formation, luteal regression, and luteal rescue by pregnancy.
authors.affiliation: Division of Obstetrics and Gynecology, Department of Reproductive, Pediatric and Infectious Science, Yamaguchi University School of Medicine, Minamikogushi 1-1-1, Ube 755-8505, Japan. sugino@yamaguchi-u.ac.jp
authors.forename: Norihiro
authors.initials: N
authors.lastname: Sugino
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Aki
authors.initials: A
authors.lastname: Sakata
authors.forename: Ichiro
authors.initials: I
authors.lastname: Miwa
authors.forename: Hiromi
authors.initials: H
authors.lastname: Asada
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Taketani
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yamagata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tamura
mesh_terms: D000199:Actins / Q000032:analysis; D000328:Adult; D042683:Angiopoietin-1 / Q000032:analysis*; D042702:Angiopoietin-2 / Q000032:analysis*; D003338:Corpus Luteum / Q000098:blood supply*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008597:Menstrual Cycle / Q000502:physiology*; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D011247:Pregnancy / Q000502:physiology*; D042787:Receptor, TIE-2 / Q000032:analysis*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: C467630:ANGPT1 protein, human; D000199:Actins; D042683:Angiopoietin-1; D042702:Angiopoietin-2; D042461:Vascular Endothelial Growth Factor A; D042787:Receptor, TIE-2