title: Inositol polyphosphate multikinase is a nuclear PI3-kinase with transcriptional regulatory activity.
abstract: Phosphatidylinositol 3,4,5-trisphosphate is a major intracellular messenger molecule thought to be formed almost exclusively by cytosolic, wortmannin-inhibited phosphoinositide 3-kinase family members. Inositol polyphosphate multikinase was identified as an enzyme that generates a series of water-soluble inositol phosphates. We now report the robust, physiologic, and evolutionarily conserved phosphoinositide 3-kinase activity of inositol polyphosphate multikinase, which is localized to nuclei and unaffected by wortmannin. In yeast, this inositol lipid kinase activity physiologically regulates transcription.
authors.affiliation: Department of Neuroscience, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.
authors.forename: Adam C
authors.initials: AC
authors.lastname: Resnick
authors.forename: Adele M
authors.initials: AM
authors.lastname: Snowman
authors.forename: Bingnan N
authors.initials: BN
authors.lastname: Kang
authors.forename: K Joseph
authors.initials: KJ
authors.lastname: Hurt
authors.forename: Solomon H
authors.initials: SH
authors.lastname: Snyder
authors.forename: Adolfo
authors.initials: A
authors.lastname: Saiardi
mesh_terms: D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology* / Q000235:genetics; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000637:Transaminases / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D000637:Transaminases; D000077191:Wortmannin; C018731:acetylornithine transaminase; C434257:inositol polyphosphate multikinase