Molecular recognition via triplex formation of mixed purine/pyrimidine DNA sequences using oligoTRIPs.
Stable DNA triple-helical structures are normally restricted to homopurine sequences. We have described a system of four heterocyclic bases (TRIPsides) that, when incorporated into oligomers (oligoTRIPs), can recognize and bind in the major groove to any native sequence of DNA [Li et al., J. Am. Chem. Soc. 2003, 125, 2084]. To date, we have reported on triplex-forming oligomers composed of two of these TRIPsides, i.e., antiTA and antiGC, and their ability to form intramolecular triplexes at mixed purine/pyrimidine sequences. In the present study, we describe the synthesis and characterization of the antiCG TRIPside and its use in conjunction with antiTA and antiGC to form sequence-specific intra- and/or intermolecular triplex structures at mixed purine/pyrimidine sequences that require as many as four major groove crossovers.
Li
Jian-Sen
JS
Eppley Institute for Research in Cancer and Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Nebraska Medical Center, Omaha, Nebraska 68198-6805, USA.
Chen
Fa-Xian
FX
Shikiya
Ronald
R
Marky
Luis A
LA
Gold
Barry
B
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D006860:Hydrogen Bonding; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D011687:Purines / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry*
D009841:Oligonucleotides; D011687:Purines; D011743:Pyrimidines; C051802:triplex DNA; D004247:DNA; C030986:pyrimidine; C030985:purine