[Hematopoietic effect of siwu decoction in the mice with blood deficiency induced by compound method of bleeding, starved feeding and exhausting].
OBJECTIVE
To observe the hematopoietic effects of Siwu decoction in the mice with blood deficiency induced by compound method of bleeding, starved feeding and exhausting and lay experimental basis under the blood-enriching function of Siwu decoction in different models of blood deficiency.


METHODS
The animal model of blood deficiency was established by a combination of starved feeding with routine food at the rate of 75 g(-1) kg(-1) body weight per day, swimming 20 minutes and removing 0.4 mL blood every other day in each mouse. The amount of peripheral blood cells, the cell cycle and the apoptosis of bone marrow cells and the percentage of CD34+ in bone marrow cells were detected by microcellcounter and flow cytometry.


RESULTS
The amount of red blood cells in model mice was significantly increased by Siwu decoction at different dosages, from 4.6 x 10(12) to 6.5, 7.1 and 7.0 x 10(12) respectively. At the 15th day, the percentages of bone marrow cells at S stage in the treated group were 14.34%, 14.03% and 13.58% which were significantly higher than that in model group without treatment of Siwu Decoction.


CONCLUSIONS
Siwu decoction significantly improve the hematopoiesis in the mice with blood deficiency induced by compound method mentioned above.
Tan
Wei
W
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Song
Chong-Shun
CS
Tan
Hong-Ling
HL
Liu
Li-Li
LL
Ma
Zeng-Chun
ZC
Wang
Yu-Guang
YG
Gao
Yue
Y
D029971:Angelica sinensis / Q000737:chemistry; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004906:Erythrocyte Count; D005221:Fatigue / Q000097:blood; D006410:Hematopoiesis / Q000187:drug effects*; D006470:Hemorrhage / Q000097:blood*; D030004:Ligusticum / Q000737:chemistry; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D032267:Rehmannia / Q000737:chemistry; D013217:Starvation / Q000097:blood
D018952:Antigens, CD34; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C403258:si-wu-tang