title: Soluble glycoprotein V as a quality marker of platelet concentrates stressed by transportation.
abstract: BACKGROUND
  Despite ongoing improvements in storage conditions for platelet concentrates (PCs) for clinical use, leukoreduced platelets (PLTs) undergo subtle changes that are partly due to PLT activation. As PLTs are activated, the expression of P-selectin (CD62P) increases, and soluble glycoprotein V (sGPV) is released. GPV, part of the GPIbIXV complex, has been suggested as a marker of PLT activation.
  
  
  METHODS
  An array of assays, used for quality control of PCs, was performed and the results were compared. The tests included PLT count, swirling, mean PLT volume, extent of shape change (ESC), hypotonic shock response (HSR), CD62P, lysosomal membrane protein (CD63), sGPV, and the metabolic tests (pH, pO(2), pCO(2), lactate, glucose). The performance of the assays was evaluated during the storage period by comparing buffy coat-derived PCs (24 PCs of 4 units) stored on flatbed agitator or stressed twice by overnight transportation.
  
  
  RESULTS
  The repeatability of all tests was good. ESC and HSR correlated with each other (r = 0.559). Importantly, there were also associations between sGPV and ESC (r = -0.564) and HSR (r = -0.389). The correlations of sGPV with lactate and glucose concentrations and with expression of CD62P and CD63 were also good. No significant changes were induced by two overnight transportations.
  
  
  CONCLUSIONS
  sGPV might be applicable for statistical process control of the quality of PCs, in addition to metabolic tests. It may also be helpful in analyzing potential improvements in blood component processing. Repeat transportation of PCs may cause minimal changes on PLT in vitro properties, if any.
authors.affiliation: Finnish Red Cross Blood Service, Kivihaantie 7, FIN-00310 Helsinki, Finland. kaija.javela@bts.redcross.fi
authors.forename: Kaija
authors.initials: K
authors.lastname: Javela
authors.forename: Juha
authors.initials: J
authors.lastname: Eronen
authors.forename: Seppo
authors.initials: S
authors.lastname: Sarna
authors.forename: Riitta
authors.initials: R
authors.lastname: Kekomäki
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D001793:Blood Preservation; D005947:Glucose / Q000493:pharmacokinetics; D006801:Humans; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000378:metabolism; D015539:Platelet Activation; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D017713:Platelet Transfusion / Q000592:standards*; D011786:Quality Control; D012995:Solubility; D013917:Thrombin / Q000494:pharmacology; D014186:Transportation
chemical_list: D015415:Biomarkers; D019038:Platelet Glycoprotein GPIb-IX Complex; D019344:Lactic Acid; D013917:Thrombin; D005947:Glucose