[Bioprotection by local and whole-body preheating--bioprotection of damage to mice tongue from burning by local preheating of oral cavity and of radiation damage of small intestine from whole-body preheating].
We have reported the cytoprotective effects of HSP 70 on various stress damage induced by mild heating. In this study, we examined the cytoprotective effects of HSP 70 induced by the local preheating of the oral cavity of mice at 42 degrees C for 30 min, and the following results (1-4) were obtained. We also examined the cytoprotective effects against radiation injury by whole-body preheating at 41.3-41.6 degrees C for 30 min (5-6). 1) The concentration of HSP 70 in lymphocytes was increased 2 days after preheating, but not significantly. 2) The concentration of HSP 70 in masseter muscle was significantly increased 2 days after preheating. 3) Under non-heat stress (control), tongue muscle was strongly stained with immunoblotting of HSP 72 antibody, an antibody of induced-type HSP 70. 4) Tongue damage and weight loss of the mice in the preheating group, whose tongues were burned, were less than in the control group. These results showed that HSP 70 induced by local preheating of the oral cavity protected against tongue damage from burning. 5) Radiation injury of the small intestine on HE stain of whole-body radiated mice was obviously reduced by whole-body preheating. 6) Decrease of the ratio of the villus length to the crypt of whole body-irradiated mice was significantly improved by whole-body preheating. From these results, it was concluded that local and whole-body preheating were useful for cytoprotection from stressful damage.
Ito
Youko H
YH
Radioisotope Research Center, Aichi Medical University.
Ippongi
Shinya
S
Nakano
Masanori
M
Kurabe
Teruhisa
T
Kazaoka
Yoshiaki
Y
Ishiguchi
Tsuneo
T
D000818:Animals; D002056:Burns / Q000517:prevention & control*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000502:physiology*; D006358:Hot Temperature / Q000627:therapeutic use*; D007421:Intestine, Small / Q000528:radiation effects*; D008297:Male; D051379:Mice; D011832:Radiation Injuries / Q000517:prevention & control*; D011835:Radiation Protection / Q000379:methods*; D014059:Tongue / Q000293:injuries*
D018840:HSP70 Heat-Shock Proteins