[Effect of human plasma on preservation of red blood cells].
In order to study whether plasma can affect the structure and function of red blood cells during their storage period, the differences of pH value, concentration of K(+), Na(+), osmotic fragility, plasma hemoglobin, AchE, ATP, 2.3-DPG, P50 in suspended RBC, washed RBC, and RBC with various plasma volume at different storage times were compared. The results showed that plasma helped the blood to keep the RBC at high pH value, low K(+), high Na(+) and maintain RBC-ATP, oxygen carry capacity and deformability, but no effect on maintenance of osmotic fragility, and levels of plasma hemoglobin, AchE, ATP and 2.3-DPG was found in preservated blood. In conclusion, human plasma may be in favour of the preservation of red blood cells.
Qiu
Yan
Y
Beijing Red Cross Blood Center, Beijing 100088, China. Qiu444cn@yahoo.com.cn
Yang
Hai-Ping
HP
Zha
Yi
Y
Li
Li-Li
LL
Li
Su-Bo
SB
Wang
De-Hai
DH
Zhang
Zi-Xin
ZX
Zhang
Yang-Pei
YP
D019794:2,3-Diphosphoglycerate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010949:Plasma / Q000502:physiology*; D011188:Potassium / Q000097:blood; D015203:Reproducibility of Results; D012964:Sodium / Q000097:blood
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate; D012964:Sodium; D011188:Potassium