title: Platelet-rich plasma influence on human osteoblasts growth.
abstract: OBJECTIVE
  The influence of progressively high concentrations of platelet-rich plasma (PRP) on human osteoblast hFOB1.19 proliferation was evaluated.
  
  
  METHODS
  The PRP was obtained from a human source. Two experiments were conducted. In the first one, PRP was diluted to 50%, 25%, 12.5% and 6.125% (v/v) with culture medium (Modified Eagle's Medium (MEM) : Ham's F12 Medium (HAM-F12%) and 1% antibiotics-antimicotic) supplemented with 10% of fetal bovine serum (FBS). In the second experiment, all conditions were identical except for the absence of FBS in the culture medium.
  
  
  RESULTS
  The results of the osteoblast proliferation test were higher when stimulated by the 50% PRP dilution, with or without FBS. A further study is suggested to determine if concentrations above 50% could cause higher rates of osteoblast proliferation. In this study, the results were not statistically different (P<0.05) with 12.5% and 6.125% PRP dilutions. Additionally, it was shown that FBS is not necessary for PRP-mediated induction of osteoblast proliferation.
  
  
  CONCLUSIONS
  This study concluded that PRP promotes osteoblast proliferation and suggested its clinical application to bone graft procedures in implant dentistry.
authors.affiliation: Department of Dental Implantology, University of Santa Catarina, Florianópolis, Brazil. cimarafortes@hotmail.com
authors.forename: Cimara Fortes
authors.initials: CF
authors.lastname: Ferreira
authors.forename: Márcia Cristina
authors.initials: MC
authors.lastname: Carriel Gomes
authors.forename: José Scarso
authors.initials: JS
authors.lastname: Filho
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Granjeiro
authors.forename: Cláudia Maria
authors.initials: CM
authors.lastname: Oliveira Simões
authors.forename: Ricardo de Souza
authors.initials: Rde S
authors.lastname: Magini
mesh_terms: D001792:Blood Platelets / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D004396:Coloring Agents; D003470:Culture Media; D016895:Culture Media, Serum-Free; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000502:physiology*; D010949:Plasma; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D004396:Coloring Agents; D003470:Culture Media; D016895:Culture Media, Serum-Free; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue