Effect of 4-hydroxynonenal on phosphatidylethanolamine containing condensed monolayer and on its interaction with apolipoprotein A-I.
4-Hydroxynonenal (4HNE), generated during polyunsaturated fatty acid oxidation, is present in atherosclerotic lesions. As 4HNE is able to react with phosphatidylethanolamine (PE), we investigated, using AC polarography, whether it may alter the physico-chemical state of a condensed PE-containing phospholipid monolayer and its interaction with apoA-I. The stability of a phospholipid monolayer relative to potential (around the potential of zero charge) is dependent on lipid composition (PE>PC>PE/PC). ApoA-I insertion into PE/PC monolayer is easier than in PC monolayer. Pre-treatment of PE/PC monolayer by 4HNE does not alter monolayer stability, but decreases apo A-I insertion into the monolayer.
Lecompte
Marie-France
MF
INSERM U-466, Biochemistry Department, IFR31, CHU Rangueil 1 Avenue Jean Poulhés, TSA 50032-31059, Toulouse Cedex 9, France. lecompte@cict.fr
Clavilier
Jean
J
Rolland
Corinne
C
Collet
Xavier
X
Nègre-Salvayre
Anne
A
Salvayre
Robert
R
D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism*; D016632:Apolipoprotein A-I / Q000737:chemistry / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000378:metabolism*
D000447:Aldehydes; D016632:Apolipoprotein A-I; D003432:Cross-Linking Reagents; D010714:Phosphatidylethanolamines; C027576:4-hydroxy-2-nonenal