Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture.
The objective of this study was to improve induction of embryogenesis in wheat microspore culture in order to obtain a high number of regenerable embryos. The arabinogalactan (AG) Larcoll and the arabinogalactan-protein (AGP) from gum arabic were tested on two spring genotypes to see if they could increase microspore viability and induce embryogenesis in the microspore culture. Adding Larcoll significantly decreased microspore mortality in both genotypes regardless of the presence or absence of ovaries in the culture. Similarly, gum arabic had a strong effect on the number of embryos produced and regenerated green plants. In fact, by using only gum arabic we were able to obtain green plants from wheat microspore cultures without the presence of ovaries. In addition to preventing a high mortality rate of the cells, our results show that the induction of embryogenesis in wheat microspore cultures is strongly affected by the use of both AG or AGP.
Letarte
Jocelyne
J
Department of Plant Agriculture, University of Guelph, Ontario, Canada, N1G 2W1.
Simion
Ecaterina
E
Miner
Mai
M
Kasha
Ken J
KJ
D003470:Culture Media; D005685:Galactans / Q000378:metabolism*; D009088:Mucoproteins / Q000502:physiology*; D010940:Plant Proteins / Q000502:physiology; D012639:Seeds / Q000254:growth & development*; D014908:Triticum / Q000196:embryology*
D003470:Culture Media; D005685:Galactans; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins; C005653:arabinogalactan