SAR by ILOEs: an NMR-based approach to reverse chemical genetics.
Reverse chemical genetics is an emerging technique that makes use of small molecule inhibitors to characterize how a protein functions. In this regard, we have developed an NMR-based approach (SAR by ILOEs) that enables the identification of high affinity ligands for a given protein target without the need of a specific assay. Our approach is of general applicability and could result very powerful in reverse chemical-genetics studies, target validation, and lead discovery. We report a recent application on the design and synthesis of compounds that inhibit protein-membrane interactions.
Becattini
Barbara
B
The Burnham Institute, 10901 North Torrey Pines Rd. La Jolla, CA 92037, USA.
Pellecchia
Maurizio
M
D002462:Cell Membrane / Q000378:metabolism; D015195:Drug Design; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000138:chemical synthesis / Q000378:metabolism; D011506:Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013329:Structure-Activity Relationship
D004364:Pharmaceutical Preparations; D011506:Proteins