title: Validation of a method to partition the base deficit in meningococcal sepsis: a retrospective study.
abstract: BACKGROUND
  The base deficit is a useful tool for quantifying total acid-base derangement, but cannot differentiate between various aetiologies. The Stewart-Fencl equations for strong ions and albumin have recently been abbreviated; we hypothesised that the abbreviated equations could be applied to the base deficit, thus partitioning this parameter into three components (the residual being the contribution from unmeasured anions).
  
  
  METHODS
  The two abbreviated equations were applied retrospectively to blood gas and chemistry results in 374 samples from a cohort of 60 children with meningococcal septic shock (mean pH 7.31, mean base deficit -7.4 meq/L). Partitioning required the simultaneous measurement of plasma sodium, chloride, albumin and blood gas analysis.
  
  
  RESULTS
  After partitioning for the effect of chloride and albumin, the residual base deficit was closely associated with unmeasured anions derived from the full Stewart-Fencl equations (r2 = 0.83, y = 1.99 - 0.87x, standard error of the estimate = 2.29 meq/L). Hypoalbuminaemia was a common finding; partitioning revealed that this produced a relatively consistent alkalinising effect on the base deficit (effect +2.9 +/- 2.2 meq/L (mean +/- SD)). The chloride effect was variable, producing both acidification and alkalinisation in approximately equal proportions (50% and 43%, respectively); furthermore the magnitude of this effect was substantial in some patients (SD +/- 5.0 meq/L).
  
  
  CONCLUSIONS
  It is now possible to partition the base deficit at the bedside with enough accuracy to permit clinical use. This provides valuable information on the aetiology of acid-base disturbance when applied to a cohort of children with meningococcal sepsis.
authors.affiliation: Department of Paediatric Intensive Care, Guy's and Saint Thomas' Hospitals, London, UK. ellen.odell@gstt.sthames.nhs.uk
authors.forename: Ellen
authors.initials: E
authors.lastname: O'Dell
authors.forename: Shane M
authors.initials: SM
authors.lastname: Tibby
authors.forename: Andrew
authors.initials: A
authors.lastname: Durward
authors.forename: Jo
authors.initials: J
authors.lastname: Aspell
authors.forename: Ian A
authors.initials: IA
authors.lastname: Murdoch
mesh_terms: D000137:Acid-Base Imbalance / Q000097:blood* / Q000209:etiology*; D001774:Blood Chemical Analysis / Q000379:methods; D002648:Child; D002675:Child, Preschool; D002712:Chlorides / Q000097:blood; D015331:Cohort Studies; D006801:Humans; D007223:Infant; D008589:Meningococcal Infections / Q000097:blood* / Q000150:complications* / Q000175:diagnosis; D012044:Regression Analysis; D015203:Reproducibility of Results; D012189:Retrospective Studies; D018805:Sepsis / Q000097:blood* / Q000150:complications*; D012709:Serum Albumin / Q000378:metabolism
chemical_list: D002712:Chlorides; D012709:Serum Albumin