Selective immobilization of fusion proteins on poly(hydroxyalkanoate) microbeads.
A novel fusion protein system employing the substrate-binding domain (SBD) of poly(hydroxyalkanoate) (PHA) depolymerase was developed for the specific immobilization of proteins on PHA microbeads, and was consequently used for immunoassays. The enhanced green fluorescent protein, red fluorescent protein, and severe acute respiratory syndrome coronavirus envelope protein were used as model proteins, and were selectively and functionally immobilized to the PHA microbeads by fusing them to the SBD. Using this PHA microbead system combined with SBD fusion technology, immunoassays could be successfully carried out.
Lee
Seok Jae
SJ
Department of Chemical & Biomolecular Engineering, BioProcess Engineering Research Center, Daejeon 305-701, Korea.
Park
Jong Pil
JP
Park
Tae Jung
TJ
Lee
Sang Yup
SY
Lee
Seongnam
S
Park
Jung Ki
JK
D005434:Flow Cytometry; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011091:Polyesters / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D011091:Polyesters; D011993:Recombinant Fusion Proteins