Cumulative and bolus in vitro contracture testing with 4-chloro-3-ethylphenol in malignant hyperthermia positive and negative human skeletal muscles.
In this study we evaluated the in vitro effects of 4-chloro-3-ethylphenol (CEP) using cumulative (12.5-200 micromol/L) or bolus (75 and 100 micromol/L) administrations, on muscle specimens from malignant hyperthermia (MH) susceptible and MH nonsusceptible patients, respectively. In the cumulative CEP in vitro contracture test, contractures were significantly greater in the MH susceptible compared with the MH nonsusceptible muscles in all concentrations between 25 and 100 micromol/L. There was no overlap between the diagnostic groups at 75 micromol/L of CEP, so this test appears to be feasible for diagnosis of MH susceptibility. The two bolus tests are not diagnostically useful, as overlaps between the diagnostic groups were observed.
Gerbershagen
Mark Ulrich
MU
*Department of Anesthesiology, Hospital Köln-Merheim, University Witten/Herdecke, Cologne, Germany; †Department of Anesthesiology, University Hospital Hamburg-Eppendorf, Martinistraβe 52, 20246 Hamburg, Germany.
Fiege
Marko
M
Weisshorn
Ralf
R
Kolodzie
Kerstin
K
Esch
Jochen Schulte Am
JSA
Wappler
Frank
F
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002733:Chlorophenols / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008305:Malignant Hyperthermia / Q000175:diagnosis / Q000503:physiopathology*; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000187:drug effects*; D011237:Predictive Value of Tests
D002733:Chlorophenols; C091710:4-chloro-3-ethylphenol; D004121:Dimethyl Sulfoxide