Targeting of mixed sequence double-stranded DNA using pyrene-functionalized 2'-amino-alpha-l-LNA.
Incorporation of a single pyrene-functionalized 2'-amino-alpha-l-LNA monomer X into short DNA strands induces extraordinarily high binding affinity towards complementary DNA (up to 16 degrees C increase per modification), whereas labile duplexes, suitable as probes for targeting of double stranded DNA, are formed upon positioning of two monomers X in an interstrand +1 zipper motif.
Hrdlicka
Patrick J
PJ
Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, DK-5230 Odense M, Denmark.
Kumar
T Santhosh
TS
Wengel
Jesper
J
D004247:DNA / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D011489:Protein Denaturation / Q000528:radiation effects; D011721:Pyrenes / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry; D014466:Ultraviolet Rays
C518188:2'-N-(pyren-1-yl)methyl-2'-amino-LNA monomer; D011721:Pyrenes; D012313:RNA; D004247:DNA; D013936:Thymidine