title: [Effects of mineral cations on the accumulation of tanshinone II A and protocatechuic aldehyde in the adventitious root culture of Salvia niltiorrhiza].
abstract: OBJECTIVE
  To study the effects of mineral cations on the growth of Salvia niltiorrhiza adventitious roots and the accumulation of tanshinone II A and protocatechuic aldehyde.
  
  
  METHODS
  The adventitious roots were cultured under different concentrations of metal cations and the contents of tanshinone II A and protocatechuic aldehyde were determined by HPLC.
  
  
  RESULTS
  Fe2 + and Mn2+ in MS medium are suitable for adventitious root growth, but Cu2+ and Mg2+ in high concentration could improve root proliferation, and Zn2+ has no obvious effect on root growth. As for tanshinone II A and protocatechuic aldehyde biosynthesis, Cu2+ and Zn2+ would inhibit the biosynthesis of protocatechuic aldehyde, furthermore, proper Fe2+ and Mg2+ concentration could advance the biosynthesis of protocatechuic aldehyde, whereas, Mn2+ in high concentration could accelerate the biosynthesis of protocatechuic aldehyde. Cu2+, Zn2+, Mg2+ in low concentration and Fe2+ and Mn2+ in high concentration would all advance the biosynthesis of tanshinone II A.
  
  
  CONCLUSIONS
  The mineral cations have obvious effects on the secondary metabolites biosynthesis in adventitious root culture of S. niltiorrhiza.
authors.affiliation: The College of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Guo
authors.forename: Wen-Yuan
authors.initials: WY
authors.lastname: Gao
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Chen
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
mesh_terms: D045784:Abietanes; D001547:Benzaldehydes / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology*; D010616:Phenanthrenes / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000254:growth & development* / Q000378:metabolism; D046509:Tissue Culture Techniques; D015032:Zinc / Q000494:pharmacology
chemical_list: D045784:Abietanes; D001547:Benzaldehydes; D002396:Catechols; D010616:Phenanthrenes; C021751:tanshinone; D008345:Manganese; C005581:protocatechualdehyde; D003300:Copper; D007501:Iron; D008274:Magnesium; D015032:Zinc