title: Development of ELISA and immunochromatographic assay for the detection of neomycin.
abstract: BACKGROUND
  Reliable analytical methods are required to monitor neomycin residue levels in the livestock products. In particular, a more simple and rapid detection method is required in the veterinary fields.
  
  
  METHODS
  Competitive direct ELISA and immunochromatographic assay were developed using monoclonal antibody to detect neomycin in the animal plasma and milk.
  
  
  RESULTS
  No cross-reactivity of the antibody was observed with other aminoglycosides based on competitive direct ELISA methods, indicating that the antibody is highly specific for neomycin. Based on the standard curves, the detection limits were determined to be 6.85 ng/ml in PBS, 3.61 ng/ml in plasma, and 2.73 ng/ml in milk, respectively. Recoveries of neomycin from spiked plasma and milk at levels of 50-200 ng/ml ranged from 87% to 108%. Concentration of intramuscularly injected neomycin was successfully monitored in the rabbit plasma through competitive direct ELISA. Immunochromatographic method was also developed using colloidal gold-conjugated monoclonal antibody. Through this method, the detection limits were estimated to be about 10 ng/ml of neomycin in PBS, plasma, and milk.
  
  
  CONCLUSIONS
  Immunochromatographic assay developed in this study is suitable for the simple screening of neomycin residues in the veterinary field. Observed positives can be confirmed using a more sensitive laboratory method such as competitive direct ELISA.
authors.affiliation: Department of Biochemistry, College of Veterinary Medicine, Seoul National University, 151-742 Seoul, South Korea.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jin
authors.forename: Jin-Wook
authors.initials: JW
authors.lastname: Jang
authors.forename: Mun-Han
authors.initials: MH
authors.lastname: Lee
authors.forename: Chang-Hoon
authors.initials: CH
authors.lastname: Han
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D002845:Chromatography / Q000295:instrumentation / Q000379:methods* / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000662:veterinary; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008892:Milk / Q000737:chemistry; D009355:Neomycin / Q000032:analysis* / Q000097:blood / Q000276:immunology; D011817:Rabbits; D015203:Reproducibility of Results
chemical_list: D000911:Antibodies, Monoclonal; D009355:Neomycin