title: The effect of supplementation with n-6 polyunsaturated fatty acids on 1-, 2- and 3-series prostaglandin F production by ovine uterine epithelial cells.
abstract: Linoleic acid (LA, 18:2n-6) has variously been found to increase or inhibit synthesis of 2-series prostaglandins (PGs), derived from arachidonic acid (AA, 20:4n-6). gamma-linolenic acid (GLA, 18:3n-6) containing oils are promoted to women for a variety of reproductive problems. Little is known concerning their actual effects on reproduction. We investigated the effects of LA, GLA and AA supplementation (25-100 microM) on basal and oxytocin (OT) stimulated production of 1-, 2- and-3 series PGs by uterine epithelial cells isolated from non-pregnant ewes, used as a model system to study endometrial PG production. PGF isomers were measured using radioimmunoassays following separation by high performance chromatography (HPLC). OT challenge increased the proportion of PGF2alpha in relation to PGF1alpha and PGF3alpha in control medium. LA supplementation decreased all PGF isomer production and reduced responsiveness to OT. GLA increased both absolute and proportional PGF1alpha production and slightly enhanced PGF2alpha generation. AA increased PGF2alpha generation and raised its isometric proportion. Both GLA and AA increased overall PGF output significantly but prevented the cells from responding to OT. These results suggest that consumption of LA and GLA are likely to differentially alter both uterine PG metabolism and responsiveness to OT. This may have implications for the control of a variety of reproductive processes.
authors.affiliation: Reproduction and Development Group, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK. zcheng@rvc.ac.uk
authors.forename: Zhangrui
authors.initials: Z
authors.lastname: Cheng
authors.forename: D Robert E
authors.initials: DR
authors.lastname: Abayasekara
authors.forename: D Claire
authors.initials: DC
authors.lastname: Wathes
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D019587:Dietary Supplements; D004717:Endometrium / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D019787:Linoleic Acid / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D010121:Oxytocin / Q000378:metabolism; D011247:Pregnancy; D011460:Prostaglandins F / Q000737:chemistry / Q000378:metabolism*; D011863:Radioimmunoassay; D012756:Sheep; D014599:Uterus / Q000033:anatomy & histology*; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism
chemical_list: D043371:Fatty Acids, Omega-6; D011460:Prostaglandins F; D017962:alpha-Linolenic Acid; D016718:Arachidonic Acid; D010121:Oxytocin; D019787:Linoleic Acid