title: Characteristics of human neural stem cells in vitro and after transplantation into rat brain.
abstract: We studied the effect of culturing conditions on the fate of human neural stem cells after transplantation into rat brain. Human neural stem cells cultured in the presence of mitogens without LIF migrated along the ependyma and cerebral vessels of recipients, but to a great extent degenerated by the 20th day after transplantation. Neural stem cells cultured with LIF migrated, apart from the above mentioned pathways, in the cortex and hippocampus, well survived; proliferating cells were retained 30 days after transplantation.
authors.affiliation: Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. aleksandrova@vigg.ru
authors.forename: M A
authors.initials: MA
authors.lastname: Aleksandrova
authors.forename: O V
authors.initials: OV
authors.lastname: Podgornyi
authors.forename: M V
authors.initials: MV
authors.lastname: Marei
authors.forename: R A
authors.initials: RA
authors.lastname: Poltavtseva
authors.forename: E B
authors.initials: EB
authors.lastname: Tsitrin
authors.forename: D V
authors.initials: DV
authors.lastname: Gulyaev
authors.forename: L V
authors.initials: LV
authors.lastname: Cherkasova
authors.forename: A V
authors.initials: AV
authors.lastname: Revishchin
authors.forename: L I
authors.initials: LI
authors.lastname: Korochkin
authors.forename: N G
authors.initials: NG
authors.lastname: Khrushchov
authors.forename: G N
authors.initials: GN
authors.lastname: Sukhikh
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D004622:Embryo, Mammalian / Q000166:cytology; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D053605:Leukemia Inhibitory Factor; D008934:Mitogens / Q000494:pharmacology; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008934:Mitogens