Yeast two-hybrid system screening.
The yeast two-hybrid system is a powerful molecular genetic tool conceived by Fields and Song. The article is a comprehensive set of methods designed to take the reader through a yeast two-hybrid analysis of your favorite gene (YFG). This article details the preparation for a screen, the screen itself, as well as the analysis of the positives identified. Using these methods, the readers should be able to successfully negotiate a yeast two-hybrid screen using the Fields' or related systems.
Gietz
R Daniel
RD
Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Canada.
D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D015723:Gene Library; D017930:Genes, Reporter; D005822:Genetic Vectors; D007763:Lac Operon; D008969:Molecular Sequence Data; D009172:Mycology / Q000379:methods; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D004271:DNA, Fungal; D001616:beta-Galactosidase