Comparison of asthma phenotypes using different sensitizing protocols in mice.
BACKGROUND
Several methods have been reported to induce asthmatic reactions in mice but few studies have compared their efficiency. We evaluated the efficiency of the protocols frequently used in the literature.


METHODS
BALB/c mice were sensitized to ovalbumin (OVA) by intraperitoneal injection; 1] Once a week for two weeks using OVA with alum (IPOA-2) or without (IPO-2), and provocation on days 28-30 by 1% OVA inhalation; 2] seven times for two weeks by OVA with alum (IPOA-7) or without (IPO-7) and provocation by 1% OVA inhalation on days 42-44. 3] Sensitization by 1% OVA inhalation for ten days (IHO-10) and provocation by 1% OVA inhalation on days 28-30. After the last challenge, airway hyperresponsiveness was measured with single chamber plethysmography 24 hours later and mice were sacrificed 48 hours later.


RESULTS
Airway hyperresponsiveness, BALF eosinophilia, airway inflammation, and OVA-specific IgE and IgG1 production were effectively induced in IPOA-2, IPOA-7, and IPO-7. However, these phenotypes were not induced in IPO-2 (except for increased BALF eosinophils) or IHO-10 (except for an increased OVA-specific IgG1 level).


CONCLUSIONS
The intraperitoneal injections of OVA with alum once a week for two weeks proved to be the most efficient sensitization method of inducing an asthmatic reaction in mice.
Chang
Yoon-Seok
YS
Department of Internal Medicine, College of Medicine, Seoul National University Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul National University Hospital, Seoul, Korea.
Kim
Yoon-Keun
YK
Bahn
Joon-Woo
JW
Kim
Sang-Heon
SH
Park
Heung-Woo
HW
Kim
Tae-Bum
TB
Cho
Sang-Heon
SH
Min
Kyung-Up
KU
Kim
You-Young
YY
D000280:Administration, Inhalation; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000097:blood; D001249:Asthma / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D001985:Bronchial Provocation Tests; D004195:Disease Models, Animal; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology; D007274:Injections, Intraperitoneal; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D010641:Phenotype
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D010047:Ovalbumin