Use of low volatility mobile phases in electroosmotic thin-layer chromatography.
A variant of electroosmotic thin-layer chromatography is suggested with the use of low volatility compounds as mobile phases aimed at drastically decreasing the evaporation of the mobile phase and improving the reproducibility of the method. The linear movement velocity of zones of separated compounds is experimentally shown to increase 2-12-fold in electroosmotic chromatography (compared to similar values in traditional TLC). The separation efficiency is also considerably increased.
Berezkin
V G
VG
A.V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninski pr 29, GSP-1, 119991 Moscow, Russia. berezkin@ips.ac.ru
Balushkin
A O
AO
Tyaglov
B V
BV
Litvin
E F
EF
D000081:Acetamides / Q000737:chemistry; D002855:Chromatography, Thin Layer / Q000379:methods*; D004396:Coloring Agents / Q000302:isolation & purification; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004126:Dimethylformamide / Q000737:chemistry; D009570:Nitriles / Q000737:chemistry; D009995:Osmosis; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry*; D014835:Volatilization
D000081:Acetamides; D004396:Coloring Agents; D009570:Nitriles; D012997:Solvents; D004126:Dimethylformamide; C013959:dimethylacetamide; D004121:Dimethyl Sulfoxide