[Effects of the glucosidorum tripterygll totorum on the proliferation and apoptosis of cell line HL-60 in human acute promyelocytic leukemia].
OBJECTIVE
To determine the effects of the glucosidorum tripterygll totorum on the proliferation inhibition and apoptosis induction of cell line HL-60 in human acute promyelocytic leukemia.


METHODS
The survival rates of cells were tested by MTT assay, fluorescence stain, flow cytometry test, and DNA fragmentation.


RESULTS
Glucosidorum tripterygll totorum could notably reduce the survival rate of HL-60 cells, and inhibit the proliferation of tumor cells. The 50% inhibitory concentration (IC50) was 5.0 microg/ml and it arrested the cell cycle at G2/M phase (P < 0. 05). The typical apoptosis morphologic cells were seen, the rates of apoptosis cells were increased (P < 0.05), and DNA ladder appeared in the experiment group.


CONCLUSIONS
Glucosidorum tripterygll totorum could notably inhibit the proliferation and induce the apoptosis of HL-60 cells.
Liu
Jing
J
Research Center qf Molecular Biology, Xiangya School of Medicine, Center South University, Changsha 410078, China.
Hu
Wei-Xin
WX
Ye
Mao
M
He
Li-Fang
LF
Zhang
Bin
B
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D029757:Tripterygium / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D005960:Glucosides