Evaluation of vasopressin mediated effects on hemostatic mechanisms: relationship with aquaporins and caveolin proteins.
With a view to evaluate the role of AQP-1 and caveolin proteins in the hemostatic actions of vasopressin, hemostasis was evaluated by bleeding and clotting time respectively. Groups of mice and guinea pigs were treated with arginine vasopressin (AVP) and 1-deamino-8D-AVP (DDAVP) to evaluate their effects on the hemostasis. DDAVP and AVP were able to appreciably reduce the bleeding and clotting time after sodium thiopentone, but not effectively after TEA treatment. Animal groups were pretreated with aquaporin-1 (AQP-1) blockers or water deprived to enhance the expression of AQP-1 water channels. Another group of animals were treated with caveolin protein modulators, cholera toxin (CTX) and the effect of vasopressin analogues evaluated. The results suggest that AQP-1 water channels and caveolin proteins contribute to modulate the hemostatic mechanisms of vasopressin.
Tyagi
Manoj G
MG
Department of Pharmacology, Christian Medical College, Vellore 632 002, India. tyagi243@yahoo.co.in
Namboodri
Keshavan V
KV
D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins / Q000037:antagonists & inhibitors / Q000502:physiology*; D001127:Arginine Vasopressin / Q000494:pharmacology*; D001760:Bleeding Time; D051242:Caveolin 1; D022461:Caveolins / Q000378:metabolism / Q000502:physiology*; D002772:Cholera Toxin / Q000494:pharmacology; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology; D006168:Guinea Pigs; D006487:Hemostasis / Q000187:drug effects* / Q000502:physiology; D006490:Hemostatics / Q000494:pharmacology*; D051379:Mice; D019789:Tetraethylammonium / Q000494:pharmacology; D014868:Water Deprivation / Q000502:physiology
C496021:Aqp1 protein, mouse; D020346:Aquaporins; C495541:Cav1 protein, mouse; D051242:Caveolin 1; D022461:Caveolins; D006490:Hemostatics; D001127:Arginine Vasopressin; D051398:Aquaporin 1; D019789:Tetraethylammonium; D002772:Cholera Toxin; D003894:Deamino Arginine Vasopressin