Gold nanoparticle-based competitive colorimetric assay for detection of protein-protein interactions.
A gold nanoparticle-based competitive colorimetric assay uses the ensemble of Concanavalin (ConA) and mannopyranoside-encapsulated gold nanoparticles (Man-GNPs) to identify the binding partners for ConA and the binding constants are determined based on the wavelength shifts.
Tsai
Charng-Sheng
CS
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Yu
Ting-Bin
TB
Chen
Chao-Tsen
CT
D003124:Colorimetry; D003208:Concanavalin A / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold / Q000737:chemistry*; D037102:Lectins; D053758:Nanoparticles; D011506:Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance; D013954:Thyroglobulin / Q000737:chemistry
D037102:Lectins; D011506:Proteins; D003208:Concanavalin A; D006046:Gold; D013954:Thyroglobulin