Association interaction and voltammetric determination of 1-aminopyrene and 1-hydroxypyrene at cyclodextrin and DNA based electrochemical sensors.
The aim of this work was voltammetric determination of 1-aminopyrene and 1-hydroxypyrene using carbon paste electrodes modified with cyclodextrin derivatives and double stranded deoxyribonucleic acid (dsDNA). The detection schemes based on a preconcentration and differential pulse voltammetric (DPV) determination at beta-cyclodextrin and gamma-cyclodextrin modified carbon paste electrode (beta-CD/CPE, gamma-CD/CPE), neutral beta-cyclodextrin polymer and carboxymethyl-beta-cyclodextrin polymer modified screen-printed electrode (beta-CDP/SPE, beta-CDPA/SPE) and dsDNA modified screen-printed electrode (DNA/SPE) are proposed for the trace determination of studied analytes within the concentration range from 2 x 10(-8) to 4 x 10(-7) mol dm(-3) and from 2 x 10(-7) to 4 x 10(-6) mol dm(-3) with the limits of quantification down to 10(-8) mol dm(-3). Depending on pH, 1-aminopyrene interacts with both surface attached CD and DNA by electrostatic bonds and supramolecular complexation while 1-hydroxypyrene associates with the CD hosts via complexation. The 1-aminopyrene interaction with dsDNA was confirmed by fluorimetric measurements in the solution phase using a competing DNA-TO-PRO-3 dye complex. In addition, the effect of temperature on this association was investigated using an electrically heated DNA-modified carbon paste electrode (DNA/CPE).
Ferancová
A
A
Department of Analytical Chemistry, Slovak University of Technology, 81237 Bratislava, Slovak Republic. adriana.ferancova@stuba.sk
Bucková
M
M
Korgová
E
E
Korbut
O
O
Gründler
P
P
Wärnmark
I
I
Stepán
R
R
Barek
J
J
Zima
J
J
Labuda
J
J
D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D003505:Cyclodextrins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D011199:Potentiometry; D011721:Pyrenes / Q000032:analysis*
D003505:Cyclodextrins; D011721:Pyrenes; D002244:Carbon; D004247:DNA; C004482:1-aminopyrene; C033146:1-hydroxypyrene