Studies on absorption and hydrolysis of ethyl alpha-D-glucoside in rat intestine.
Ethyl alpha-D-glucoside (alpha-EG) is normally contained in Sake, which has been taken by Japanese people since ancient times. In this study, the intestinal absorption of alpha-EG was investigated using rat everted intestinal sac. Furthermore, the alpha-EG hydrolytic activity in rat intestine was compared with disaccharides hydrolytic activities, and the effects of alpha-EG on disaccharides hydrolysis were examined using crude enzyme preparation from rat intestinal acetone powder. Glucose liberated from alpha-EG was detected in a serosal solution of everted rat intestinal sac, but it was only less than 4% of absorbed intact alpha-EG. alpha-EG absorption into small intestinal tissue was reduced by elimination of sodium ion from the mucosal solution or under the presence of phlorizin. The hydrolytic activity for alpha-EG was detected in crude enzyme preparation from rat intestinal acetone powder, but it showed a low value as compared to those for disaccharides. alpha-EG showed mixed type inhibition for maltose and sucrose hydrolysis, but inhibitory concentrations of alpha-EG required for 50% inhibition for the maltose and sucrose hydrolysis were higher than those of arabinose and acarbose. In conclusion, a small amount of alpha-EG was hydrolyzed and most of it was absorbed via SGLT1 as an intact form in the rat small intestine, and the inhibitory effect of alpha-EG on disaccharides hydrolysis was weak.
Mishima
Tomoyuki
T
The United Graduate School of Agricultural Science and Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. h6103007@cc.gifu-u.ac.jp
Tanaka
Keiko
K
Tsuge
Haruhito
H
Sugita
Jun
J
Nakahara
Michio
M
Hayakawa
Takashi
T
D000818:Animals; D004187:Disaccharides / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005960:Glucosides / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D007408:Intestinal Absorption; D007422:Intestines / Q000201:enzymology*; D007700:Kinetics; D051381:Rats; D017208:Rats, Wistar
D004187:Disaccharides; D005960:Glucosides; C108400:ethyl glucoside; D005947:Glucose