CpG-oligodeoxynucleotide protects immune cells from gamma-irradiation-induced cell death.
Synthetic oligodeoxynucleotides (CpG-ODNs) and bacterial DNA containing unmethylated CpG dinucleotides in the context of particular base sequences (CpG motifs) are known to inhibit anti-IgM-induced growth arrest and apoptosis of WHEI 231 B lymphocytes, and spontaneous apoptosis of mature spleen B cells in a sequence-specific fashion of the CpG-ODN. Here we report that CpG-ODN protects from the cell death induced by gamma-irradiation of primary mouse spleen cells as well as mouse RAW 264.7 macrophage cells and human RPMI 8226 B cells. Experimental results showed that CpG-ODN promotes growth of the cells, and protects the cells from gamma-irradiation-induced cell death accompanying Bcl-xS/L and Bcl-2 upregulation. Furthermore, survival of macrophages was enhanced when splenocytes were pretreated with CpG-ODN. Our results suggest the potential application of CpG-ODNs for more efficient cancer radiotherapy by enhancing survival of normal immune cells after radiation damage.
Sohn
Wern-Joo
WJ
Department of Microbiology, College of Medicine, Hallym University, 39 Hallymdaehak-gil, Chuncheon 200-702, Republic of Korea.
Lee
Keun-Wook
KW
Choi
Soo Young
SY
Chung
Eunkyung
E
Lee
Younghee
Y
Kim
Tae Yoon
TY
Lee
Suk Kyeong
SK
Choe
Yong-Kyoung
YK
Lee
Jeung-Hoon
JH
Kim
Doo-Sik
DS
Kwon
Hyung-Joo
HJ
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000528:radiation effects*; D016923:Cell Death / Q000528:radiation effects; D002470:Cell Survival; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D013997:Time Factors; D015854:Up-Regulation / Q000235:genetics; D051020:bcl-X Protein / Q000235:genetics
C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein