[Experimental study on anti-inflammatory effects of mahuang decoction and its compositions].
OBJECTIVE
To explore the regularity of recipe composition by observing anti-inflammatory effects of Mahuang Decoction (MHD) and its compositions.


METHODS
Neutrophil aggregation was induced by intraperitoneal injection of glycogen in rats. Intraperitoneal lavage fluid (PLF) was collected and neutrophils were removed. Neutrophils were stimulated by calciumionophore A23187 in vitro to produce leukotriene B4. The concentration of leukotriene B4 was measured by reversed-phase HPLC, anti-inflammatory effect was observed by the auricular edema with dimethylbenze.


RESULTS
MHD and its compositions could inhibit the auricular edema with dimethylbenze and leukotriene production from neutrophils in rats. Inhibitory effects of MHD compositions were different.


CONCLUSIONS
MHD had significantly inhibitory effects on the auricular edema with dimethylbenze and leukotriene production from neutrophils in rats. The original formula (MHD) worked best. These result confirmed the rationality and scientific level of MHD.
Liu
Yonggang
Y
Key Laboratory of Pharmaceutical of Materia Medica, Nanfang Medical University, Guangzhou.
Luo
Jiabo
J
He
Feng
F
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002449:Cell Aggregation; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D007975:Leukotriene B4 / Q000378:metabolism*; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010031:Otitis / Q000139:chemically induced; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014992:Xylenes
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; D014992:Xylenes; D007975:Leukotriene B4