title: Hepatotoxic effects of tert-butyl hydroperoxide (t-BHP) and protection by antioxidants.
abstract: t-BHP induced oxidative stress and Ca2+ function impairment in fresh hepatocytes was studied in order to understand its role in cytotoxicity. Viability of hepatocytes by the release of lactate dehydrogenase and methyl thiazoletetrazolium reduction method alongwith malondialdehyde formation indicated oxidative stress in the hepatotoxic action of t-BHP.
authors.affiliation: Department of Biochemistry, Dr. R. M. L. Avadh University, Faizabad 224 001, India.
authors.forename: Sangram
authors.initials: S
authors.lastname: Singh
authors.forename: Sudhir
authors.initials: S
authors.lastname: Mehrotra
authors.forename: Rajeev
authors.initials: R
authors.lastname: Pandey
authors.forename: Rajat
authors.initials: R
authors.lastname: Sandhir
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D008054:Lipid Peroxides; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide