[Effect of Angelica sinensis polysaccharide on lymphocyte proliferation and cytokine induction].
OBJECTIVE
To investigate the effects of Angelica sinensis polysaccharide (AP) and its three fractions (AP-1, AP-2 and AP-3) on lymphocyte proliferation induction of IL-2 and IFN-gamma.


METHODS
Effects of AP on proliferation of murine splenocyte, macrophage, mixed lymphocyte reaction were tested by MTT assay. The production of IL-2 and IFN-gamma in supernatant were determined by ELISA.


RESULTS
At the range of 30 - 300 microg/ml, AP and its three fractions directly stimulated proliferation of murine splenocyte; AP-3 significantly enhanced the proliferation of macrophage and mixed lymphocyte, the treatment of AP-3 augmented the production of IL-2 and IFN-gamma in cell culture supernatant.


CONCLUSIONS
AP could active macrophage and T lymphocyte, also regulate the immune response through upregulating IL-2, IFN-gamma expression.
Yang
Tiehong
T
Department of Pharmacology, Fourth Military Medical University, Xi'an.
Jia
Min
M
Mei
Qibing
Q
D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000276:immunology; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000166:cytology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology*
D007376:Interleukin-2; D011134:Polysaccharides; D007371:Interferon-gamma