[The isolation and culture of Sertoli cells from adult testis and their immune privilege mechanism].
OBJECTIVE
To set up a method of isolation and culture of Sertoli cells from adult testis and to investigate their immune privilege mechanism.


METHODS
Adult testis Sertoli cells were prepared successfully by digestion with trypsin, collagenase, hyaluronidase and DNase, and then the expression of Fas-L and TGF-beta1 was examined by SABC staining. The Sertoli cells were cocultured with adult splenocytes to detect the inhibitory effect of Sertoli cells on splenocyte proliferation by MTT colorimetry.


RESULTS
In cultured cells, Sertoli cells accounted for more than 80% of total cells. The activity of Sertoli cells reached 90%. Sertoli cells expressed Fas-L and TGF-beta1 and could inhibit the proliferation of cocultured splenocytes in vitro.


CONCLUSIONS
A method of isolation and culture of Sertoli cells from adult testis has been established. The immune privilege mechanism of Sertoli cells may be related to the expression of Fas-L and TGF-beta1.
Teng
Yan
Y
Department of Kidney Transplantation, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. tengyan2000@163.com
Xue
Wu-jun
WJ
Feng
Xin-shun
XS
Tian
Pu-xun
PX
Li
Zhao-lun
ZL
D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D053222:Fas Ligand Protein / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008297:Male; D012708:Sertoli Cells / Q000166:cytology* / Q000276:immunology*; D013737:Testis / Q000166:cytology*; D053773:Transforming Growth Factor beta1 / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
D053222:Fas Ligand Protein; D053773:Transforming Growth Factor beta1