Electrophoretically separation of the synovial fluid proteins in rabbit temporomandibular arthritis induced by mechanical loading.
BACKGROUND
The concentration of protein in synovial fluid (SF) of temporomandibular joints (TMJs) with disorders tends to be increased. We investigated the protein composition of SF of rabbits in which arthritis of the TMJ was induced.


METHODS
Arthritis was induced in six TMJs in six rabbits by exertion of a load for 4 weeks. Six non-loaded TMJs in six rabbits served as controls. The protein concentration and content in TMJ SF of the two groups were compared.


RESULTS
The mean protein concentration was higher in the SF of the loaded group than in that of the non-loaded group (1824 microg/ml vs. 398 microg/ml, P = 0.002). Proteins with molecular weights of more than 95 kDa were abundant in the loaded group (P < 0.05).


CONCLUSIONS
Temporomandibular arthritis induced by mechanical loading in rabbit is accompanied by an increase in the abundance of relatively high molecular weight proteins in SF.
Fujimura
K
K
Department of Oral and Maxillofacial Surgery, Kanazawa Medical University, Ishikawa, Japan. l-kkk@kanazawa-med.ac.jp
Kobayashi
S
S
Yoshitake
Y
Y
Tsuruoka
N
N
Kaneyama
K
K
Segami
N
N
D000818:Animals; D001169:Arthritis, Experimental / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D001798:Blood Proteins / Q000032:analysis; D001864:Bone Wires; D016207:Cytokines / Q000032:analysis / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008335:Mandibular Condyle / Q000473:pathology; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D013314:Stress, Mechanical; D013582:Synovial Fluid / Q000737:chemistry*; D013585:Synovitis / Q000473:pathology; D013701:Temporal Bone / Q000473:pathology / Q000503:physiopathology; D013705:Temporomandibular Joint Disorders / Q000378:metabolism* / Q000473:pathology
D001798:Blood Proteins; D016207:Cytokines; D011506:Proteins