title: [Construction of a new vector for direct cloning of PCR products].
abstract: A new method for construction of a cloning vector (T-vector) for direct ligation with PCR products was described. The T-vector derived from pUC118 in which the unique restriction site of Eam1105 I in the region of Amp(r) gene was deleted and an artificial DNA fragment flanking two Eam1105 I was introduced at the site of BamHI. The modified vector was named as pUC118E. A T-vector with 3' over hang end of a single T can be obtained via digesting of pUC118E with Eam1105I. PCR products can be easily cloned with this T-vector.
authors.affiliation: Medical College of Dalian University, Dalian 116622, China. huxuejun@mail.dlptt.ln.cn
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Hu
authors.forename: Jing-Quan
authors.initials: JQ
authors.lastname: Li
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Yuan
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Xu
authors.forename: Dong-Li
authors.initials: DL
authors.lastname: Zhao