title: [The isolation and culture of Sertoli cells from adult testis and their immune privilege mechanism].
abstract: OBJECTIVE
  To set up a method of isolation and culture of Sertoli cells from adult testis and to investigate their immune privilege mechanism.
  
  
  METHODS
  Adult testis Sertoli cells were prepared successfully by digestion with trypsin, collagenase, hyaluronidase and DNase, and then the expression of Fas-L and TGF-beta1 was examined by SABC staining. The Sertoli cells were cocultured with adult splenocytes to detect the inhibitory effect of Sertoli cells on splenocyte proliferation by MTT colorimetry.
  
  
  RESULTS
  In cultured cells, Sertoli cells accounted for more than 80% of total cells. The activity of Sertoli cells reached 90%. Sertoli cells expressed Fas-L and TGF-beta1 and could inhibit the proliferation of cocultured splenocytes in vitro.
  
  
  CONCLUSIONS
  A method of isolation and culture of Sertoli cells from adult testis has been established. The immune privilege mechanism of Sertoli cells may be related to the expression of Fas-L and TGF-beta1.
authors.affiliation: Department of Kidney Transplantation, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. tengyan2000@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Teng
authors.forename: Wu-jun
authors.initials: WJ
authors.lastname: Xue
authors.forename: Xin-shun
authors.initials: XS
authors.lastname: Feng
authors.forename: Pu-xun
authors.initials: PX
authors.lastname: Tian
authors.forename: Zhao-lun
authors.initials: ZL
authors.lastname: Li
mesh_terms: D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D053222:Fas Ligand Protein / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008297:Male; D012708:Sertoli Cells / Q000166:cytology* / Q000276:immunology*; D013737:Testis / Q000166:cytology*; D053773:Transforming Growth Factor beta1 / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
chemical_list: D053222:Fas Ligand Protein; D053773:Transforming Growth Factor beta1