Fluorous affinity purification of oligonucleotides.
[structure: see text] Nucleoside phosphoramidites bearing a fluorous dimethoxytrityl (FDMT) group were used to synthesize fluorous-tagged oligonucleotides, which were subjected to solid-phase extraction using a pH-stable fluorinated adsorbent. On-column detritylation afforded the purified oligonucleotides. The fluorous affinity purification method offers one-pass loading without ammonia removal, high selectivity for the removal of failure sequences, high recoveries (typically 70-100%), and the ability to purify long oligonucleotides (e.g., 50-100-mers).
Pearson
William H
WH
Berry & Associates, Inc., 2434 Bishop Circle East, Dexter, Michigan 48130, USA. wpearson@berryassoc.com
Berry
David A
DA
Stoy
Patrick
P
Jung
Kee-Yong
KY
Sercel
Anthony D
AD
D000345:Affinity Labels / Q000737:chemistry*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D005461:Fluorine / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000302:isolation & purification*
D000345:Affinity Labels; D009841:Oligonucleotides; D005461:Fluorine