A human protein atlas for normal and cancer tissues based on antibody proteomics.
Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, approximately 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.
Uhlén
Mathias
M
Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH), SE-106 91 Stockholm, Sweden. mathias.uhlen@biotech.kth.se
Björling
Erik
E
Agaton
Charlotta
C
Szigyarto
Cristina Al-Khalili
CA
Amini
Bahram
B
Andersen
Elisabet
E
Andersson
Ann-Catrin
AC
Angelidou
Pia
P
Asplund
Anna
A
Asplund
Caroline
C
Berglund
Lisa
L
Bergström
Kristina
K
Brumer
Harry
H
Cerjan
Dijana
D
Ekström
Marica
M
Elobeid
Adila
A
Eriksson
Cecilia
C
Fagerberg
Linn
L
Falk
Ronny
R
Fall
Jenny
J
Forsberg
Mattias
M
Björklund
Marcus Gry
MG
Gumbel
Kristoffer
K
Halimi
Asif
A
Hallin
Inga
I
Hamsten
Carl
C
Hansson
Marianne
M
Hedhammar
My
M
Hercules
Görel
G
Kampf
Caroline
C
Larsson
Karin
K
Lindskog
Mats
M
Lodewyckx
Wald
W
Lund
Jan
J
Lundeberg
Joakim
J
Magnusson
Kristina
K
Malm
Erik
E
Nilsson
Peter
P
Odling
Jenny
J
Oksvold
Per
P
Olsson
Ingmarie
I
Oster
Emma
E
Ottosson
Jenny
J
Paavilainen
Linda
L
Persson
Anja
A
Rimini
Rebecca
R
Rockberg
Johan
J
Runeson
Marcus
M
Sivertsson
Asa
A
Sköllermo
Anna
A
Steen
Johanna
J
Stenvall
Maria
M
Sterky
Fredrik
F
Strömberg
Sara
S
Sundberg
Mårten
M
Tegel
Hanna
H
Tourle
Samuel
S
Wahlund
Eva
E
Waldén
Annelie
A
Wan
Jinghong
J
Wernérus
Henrik
H
Westberg
Joakim
J
Wester
Kenneth
K
Wrethagen
Ulla
U
Xu
Lan Lan
LL
Hober
Sophia
S
Pontén
Fredrik
F
D000906:Antibodies / Q000737:chemistry* / Q000302:isolation & purification; D000912:Antibodies, Neoplasm / Q000737:chemistry* / Q000302:isolation & purification; D015153:Blotting, Western; D002846:Chromatography, Affinity; D030562:Databases, Protein; D000939:Epitopes / Q000737:chemistry; D020224:Expressed Sequence Tags; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000276:immunology*; D011506:Proteins / Q000276:immunology; D020543:Proteome / Q000276:immunology* / Q000302:isolation & purification; D012016:Reference Values
D000906:Antibodies; D000912:Antibodies, Neoplasm; D000939:Epitopes; D011506:Proteins; D020543:Proteome