title: Changes in cellular secretory processing during baculovirus infection.
abstract: In the baculovirus expression vector system (BEVS), secretory green fluorescent protein (sGFP) transcripts were expressed from day 2 to day 5 post-infection (p.i.), while transcripts of the endoplasmic reticulum (ER) molecular chaperone Bombyx mori protein disulfide isomerase (bPDI) were measurable in mock cells and in cells at day 1 p.i. The GFP was expressed from day 3 to day 5 p.i. whereas the levels of two ER chaperone proteins, bPDI and calnexin, decreased from day 3 p.i. and were not detected from day 4 p.i. These findings suggest that the rate-limited expression of ER molecular chaperones is strongly associated with the maximal expression of exogenous proteins in BEVS.
authors.affiliation: Department of Agricultural Biology, National Institute of Agricultural Science and Technology, 441-100, RDA, Suwon, Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Yun
authors.forename: Tae-Won
authors.initials: TW
authors.lastname: Goo
authors.forename: Sung-Wan
authors.initials: SW
authors.lastname: Kim
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Choi
authors.forename: Jae-Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Seok-Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: O-Yu
authors.initials: OY
authors.lastname: Kwon
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000254:growth & development*; D012831:Bombyx / Q000201:enzymology* / Q000821:virology; D037282:Calreticulin / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology* / Q000378:metabolism; D019476:Insect Proteins / Q000378:metabolism*; D019704:Protein Disulfide-Isomerases / Q000378:metabolism*
chemical_list: D037282:Calreticulin; D019476:Insect Proteins; D019704:Protein Disulfide-Isomerases