title: [Hydrolysis and mechanism of wastewater contained protein using immobilized protein enzyme].
abstract: Protein-enzyme was immobilized on big-hole resin, activated charcoal and silica particles by a absorption method. This study focuses on effect of enzyme immobilization, thermal stability and hydrolysis efficiency of wastewater. The result of batch test show that the ratio of immobilized enzyme on activated charcoal, big-hole resin and silica particles was 61.95%, 37.85% and 20.31% respectively. The highest of thermal ability of activated charcoal, big-hole resin and silica particles was 18.68 mg/min, 21.48 mg/min and 3.6 mg/min respectively at pH 5.5. The total amino-acids of big-hole resin and activated charcoal immobilized enzyme after hydrolysis was respectively increased to 21.26 times and 14.6 times. The kind of amino-acid was increased from 14 to 16 kinds. The continuous-flow test result indicate that concentration of substrate in influent had significant effect on the hydrolysis rate of immobilized enzyme reactor. The reactor was able to gain hydrolysis rate of 80% for protein and 36.1% (VFA/COD%) for organic materials in the wastewater with a moderate concentration of protein (500 mg/L). The ethanol and acetic of volatile hydrolysis-mixture composition were 50.3% and 33.3% respectively.
authors.affiliation: College of Environment and Resource, Jilin University, Changchun 130026, China.
authors.forename: Hong-bing
authors.initials: HB
authors.lastname: Yu
authors.forename: Xue-yu
authors.initials: XY
authors.lastname: Lin
authors.forename: Lan-ying
authors.initials: LY
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Yang
authors.forename: Ji-li
authors.initials: JL
authors.lastname: Liu
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry; D006868:Hydrolysis; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D014865:Waste Disposal, Fluid / Q000379:methods*; D014873:Water Pollutants / Q000032:analysis* / Q000737:chemistry; D018508:Water Purification / Q000379:methods
chemical_list: D004800:Enzymes, Immobilized; D011506:Proteins; D014873:Water Pollutants