title: Rice Undeveloped Tapetum1 is a major regulator of early tapetum development.
abstract: The tapetum, the innermost of four sporophytic layers in the anther wall, comes in direct contact with the developing male gametophyte and is thought to play a crucial role in the development and maturation of microspores. Here, we report the identification of rice (Oryza sativa) Undeveloped Tapetum1 (Udt1), which is required for the differentiation of secondary parietal cells to mature tapetal cells. T-DNA or retrotransposon Tos17 insertions in the Udt1 gene caused male sterility. The anther walls and meiocytes of the mutants were normal during the early premeiosis stage, but their tapeta failed to differentiate and became vacuolated during the meiotic stage. In addition, meiocytes did not develop to microspores, and middle layer degeneration was inhibited. Consequently, the anther locules contained no pollen. The UDT1:green fluorescent protein fusion protein was localized to the nucleus. This, together with its homology with other basic helix-loop-helix proteins, suggests that UDT1 is a transcription factor. DNA microarray analysis identified 958 downregulated and 267 upregulated genes in the udt1-1 anthers, suggesting that Udt1 plays a major role in maintaining tapetum development, starting in early meiosis.
authors.affiliation: National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea. jkhim@postech.ac.kr
authors.forename: Ki-Hong
authors.initials: KH
authors.lastname: Jung
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Han
authors.forename: Yang-Seok
authors.initials: YS
authors.lastname: Lee
authors.forename: Yong-Woo
authors.initials: YW
authors.lastname: Kim
authors.forename: Inhwan
authors.initials: I
authors.lastname: Hwang
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Yeon-Ki
authors.initials: YK
authors.lastname: Kim
authors.forename: Baek Hie
authors.initials: BH
authors.lastname: Nahm
authors.forename: Gynheung
authors.initials: G
authors.lastname: An
mesh_terms: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D035264:Flowers / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D005854:Germ Cells / Q000254:growth & development / Q000378:metabolism; D008540:Meiosis / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D051479:Plant Infertility / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D050436:Regulatory Elements, Transcriptional / Q000235:genetics; D018626:Retroelements / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013170:Spores / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D004269:DNA, Bacterial; D009687:Nuclear Proteins; D010940:Plant Proteins; D018626:Retroelements; C036483:T-DNA; D014157:Transcription Factors