[The light diffusion technique for estimation of the size of urine particles in patients with type 2 diabetes mellitus in the Tamm-Horsfall protein precipitation].
Tamm-Horsfall uroprotein accounts for more than 50% of the urinary proteins in healthy individuals. In abnormalities, it creates a favorable background for detecting smaller-sized uroproteins and for diagnosing pathological processes from the results of native urine tests. In this connection, there is a need for precipitating Tamm-Horsfall glycoprotein while applying laser correlation spectroscopy to analyze the size of urine particles in patients with type 2 diabetes mellitus. Eighty patients with this condition concurrent with different stages of diabetic nephropathy and 23 apparently healthy individuals were examined. The findings suggest that the subfraction urine composition before and after Tamm-Horsfall protein precipitation is different in apparently healthy individuals and patients with type 2 diabetes mellitus concurrent with diabetic nephropathy. This is most likely to be due to the change in the qualitative composition of protein as renal lesion progresses, to the specific features of protein excretion at different stages of a pathological process, and to different concentrations of other low and high molecular-weight proteins.
Zakharchenko
V M
VM
Khorovskaia
L A
LA
Emanuél'
V L
VL
Shustov
S B
SB
Nagibovich
O A
OA
Landa
S B
SB
Arutiunian
A V
AV
Gupalova
T V
TV
Palagniuk
V G
VG
D000328:Adult; D011232:Chemical Precipitation; D003924:Diabetes Mellitus, Type 2 / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D009088:Mucoproteins / Q000737:chemistry; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011237:Predictive Value of Tests; D011506:Proteins / Q000032:analysis*; D058949:Uromodulin
D009088:Mucoproteins; D011506:Proteins; C548612:UMOD protein, human; D058949:Uromodulin