Genomic fingerprinting of antituberculosis agents-resistant Lactobacillus ruminus SPM0211 using the Microbial Uniprimer kit.
A Lactobacillus isolate was collected from the feces of a healthy Korean individual and named as Lactobacillus ruminus SPM0211. It was further characterized by subjecting it to an antibiotic resistance test and genetic analysis. In the antibiotic resistance test, all tested Lactobacillus spp. were classified as "high resistance" for multiple antibiotics, such as isoniazid, ethambutol, cycloserine, and vancomycin. L. ruminus SPM0211 was classified as "high resistance" for streptomycin also, while the other tested Lactobacillus spp. were classified as low resistance. This suggests that the antimicrobial spectra may be a good indicator in the discrimination of this strain among the tested Lactobacillus spp. In a polymerase chain reaction-random amplified polymorphic DNA (PCR-RAPD) analysis using the Microbial Uniprimer kit, L. ruminus SPM0211, and L. suebicus were clustered as a group with a 74.3% similarity level, suggesting that these two species are genetically related. Thus, our data suggest that the PCR-RADP method using the Microbial Uniprimer kit may be valuable in discriminating L. ruminus SPM0211 from other Lactobacillus spp.
Kang
Byung Yong
BY
Research Institute for Life Science, Sahmyook University, Seoul 139-742, Korea.
Song
Moon Seok
MS
Kim
Yun A
YA
Park
So Hee
SH
Chung
Myung Jun
MJ
Kim
Soo Dong
SD
Baek
Dae Heoun
DH
Kim
Kyungjae
K
Ha
Nam Joo
NJ
D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000995:Antitubercular Agents / Q000494:pharmacology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004977:Ethambutol / Q000494:pharmacology; D005243:Feces / Q000382:microbiology*; D005798:Genes, Bacterial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D007778:Lactobacillus / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D011933:Reagent Kits, Diagnostic; D013307:Streptomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D000995:Antitubercular Agents; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; D004977:Ethambutol; D007538:Isoniazid; D013307:Streptomycin