Highly active and stable DNAzyme-carbon nanotube hybrids.
We report herein the generation and characterization of DNAzyme-carbon nanotube conjugates. Biotinylated DNAzyme was attached to streptavidin-functionalized MWNTs. The resultant DNAzyme-MWNT conjugates exhibited Michaelis-Menten kinetics and multiple catalytic turnovers. The specific activity of the nanoscale conjugates was approximately 1/3 of that for freely soluble DNAzyme. Such high activity may allow myriad applications ranging from the directed assembly of nanomaterials to nanoscale cellular therapeutics that rely on the RNA cleaving ability of DNAzymes.
Yim
Tae-Jin
TJ
Department of Chemical and Biological Engineering and Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Liu
Juewen
J
Lu
Yi
Y
Kane
Ravi S
RS
Dordick
Jonathan S
JS
D002384:Catalysis; D003461:Crystallography; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D013997:Time Factors
D021881:DNA, Catalytic; D037742:Nanotubes, Carbon; D019809:Streptavidin