title: A carotenoid cleavage dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation.
abstract: A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy-beta-ionone, a C(13)-norisoprenoidic compound, and a C(14)-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching véraison was observed in both cultivars. In parallel, the C(13)-norisoprenoid level increased from véraison to maturity in both cultivars.
authors.affiliation: UMR IR2B, ENSAM-INRA-Université Montpellier II, F-34060 Montpellier cedex 1, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Mathieu
authors.forename: Nancy
authors.initials: N
authors.lastname: Terrier
authors.forename: Jérôme
authors.initials: J
authors.lastname: Procureur
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bigey
authors.forename: Ziya
authors.initials: Z
authors.lastname: Günata
mesh_terms: D002247:Carbon Isotopes; D018076:DNA, Complementary / Q000235:genetics; D049308:Dioxygenases / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli; D020224:Expressed Sequence Tags; D005638:Fruit / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D045792:Norisoprenoids / Q000378:metabolism*; D010802:Phylogeny; D027843:Vitis / Q000201:enzymology* / Q000235:genetics*
chemical_list: D002247:Carbon Isotopes; D018076:DNA, Complementary; D045792:Norisoprenoids; D049308:Dioxygenases; C431939:carotenoid cleavage dioxygenase 1