title: Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent.
abstract: Fosinopril sodium presents a prodrug for the active angiotensin converting enzyme (ACE) inhibitor, fosinoprilat. The dual elimination of fosinoprilat by the liver and the kidney distinguishes fosinopril from other angiotensin converting enzyme inhibitors. Such ways of elimination are important for antihypertensive therapy of patients on haemodialysis. The paper presents development and evaluation of a new and sensitive liquid chromatographic (LC) method for the analysis of fosinoprilat in plasma obtained from patients on haemodialysis. A microemulsion system mixture as mobile phase has been used for the separation and analysis of fosinoprilat in plasma samples. The plasma samples were injected directly onto the HPLC system (Waters Breeze) after appropriate sample dilution with mobile phase. Separations were performed on the Bakerbond ENV 4.6 mm x 150 mm, 5 microm particle size column with UV detection at 220 nm. The flow rate was 1.00 mL min(-1). The mobile phase consisted of 1.0% (w/v) of diisopropyl ether, 2.0% (w/v) of sodium dodecyl sulphate (SDS), 6.0% (w/v) of n-propanol and 91% (w/v) of aqueous 25 mM di-sodium hydrogen phosphate, pH adjusted to 2.8 with 85% orthophosphoric acid. The developed method was then subjected to method validation according to the criteria stated in the FDA bioanalytical method validation guidance. The results for specificity, linearity, low limit of quantification (LLOQ), precision, accuracy and stability were within the accepted criteria. The unique approach applied in this paper makes possible the determination of fosinoprilat even in the presence of metabolites of other drugs, so the method can be used for obtaining the reliable results in a fast and simple way.
authors.affiliation: Faculty of Pharmacy, Department of Drug Analysis, Vojvode Stepe 450, Belgrade, Serbia and Montenegro.
authors.forename: B
authors.initials: B
authors.lastname: Jancic
authors.forename: D
authors.initials: D
authors.lastname: Ivanovic
authors.forename: M
authors.initials: M
authors.lastname: Medenica
authors.forename: A
authors.initials: A
authors.lastname: Malenovic
authors.forename: N
authors.initials: N
authors.lastname: Dimkovic
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004655:Emulsions; D017328:Fosinopril / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D006435:Renal Dialysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D004655:Emulsions; D017328:Fosinopril; C046965:fosinoprilat