[Investigation of immunological mechanism of the in vivo anti-tumor effect of K8 (C6H11N3O2)2[SiW9Ti3O40]] (WT)].
OBJECTIVE
To investigate the in vivo anti-tumor effect of K(8)(C(6)H(11)N(3)O(2))(2)[SiW(9)Ti(3)O(40)] (WT) and the immunological mechanism.


METHODS
WT was administrated intragastrically to mice bearing H22 hepatocarcinoma for 10 days and then the tumors were isolated and weighed. MTT colorimetry was used to detect lymphocyte transformation function and cytotoxicity of NK cells. The morphology of apoptotic tumor cells was observed under phase contrast microscope and light microscope after HE staining. The apoptosis rate of BEL-7402 cells treated with WT was detected by flow cytometry.


RESULTS
Compared to the control group, WT obviously inhibited the growth of H22 tumor in tumor bearing mice (P<0.05). It enhanced significantly lymphocyte transformation function and cytotoxicity of NK cells (P<0.05). Apoptotic tumor cells were observed under microscope and detected by flow cytometry.


CONCLUSIONS
WT inhibits significantly the growth of tumor by up-regulating the activity of immunocytes and inducing apoptosis of tumor cells.
Zhang
Song-ling
SL
Department of Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
Mei
Shao-lin
SL
Chang
Ya-ping
YP
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000276:immunology*; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008858:Microscopy, Phase-Contrast
D000970:Antineoplastic Agents