title: Potential roles of follicular dendritic cell-associated osteopontin in lymphoid follicle pathology and repair and in B cell regulation in HIV-1 and SIV infection.
abstract: Osteopontin is a multifunctional protein with known roles in bone remodeling, wound healing, and normal and pathological immune responses. We showed in microarray studies that osteopontin gene expression is increased in human immunodeficiency virus type 1 (HIV-1)-infected lymphatic tissues after treatment, and we undertook mapping experiments to study osteopontin's possible functions in this context. We discovered species-specific colocalization of osteopontin with the follicular dendritic cell (FDC) network in lymphatic tissues in HIV-1 and simian immunodeficiency virus infections, and we found that changes in FDC-associated osteopontin covary with changes in lymphoid follicles during acute and late stages of infection and in response to treatment. We propose that this localization normally facilitates antibody production and plays a role in B cell abnormalities in infection and in the reconstitution of lymphoid follicles with treatment and that mapping genes identified in microarray studies is a useful experimental approach to gaining a better understanding of function in the context of a particular tissue and disease.
authors.affiliation: Department of Microbiology, University of Minnesota, Minneapolis, MN 55455 , USA.
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Li
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Lifson
authors.forename: Lijie
authors.initials: L
authors.lastname: Duan
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Schacker
authors.forename: Cavan
authors.initials: C
authors.lastname: Reilly
authors.forename: John
authors.initials: J
authors.lastname: Carlis
authors.forename: Jacob D
authors.initials: JD
authors.lastname: Estes
authors.forename: Ashley T
authors.initials: AT
authors.lastname: Haase
mesh_terms: D000818:Animals; D023241:Antiretroviral Therapy, Highly Active; D001402:B-Lymphocytes / Q000276:immunology*; D001706:Biopsy; D020566:Dendritic Cells, Follicular / Q000378:metabolism / Q000473:pathology* / Q000502:physiology* / Q000821:virology; D005260:Female; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology / Q000821:virology; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000378:metabolism; D015302:Simian Immunodeficiency Virus / Q000472:pathogenicity; D013045:Species Specificity
chemical_list: C506895:SPP1 protein, human; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin