Ligation of CD47 mediates phosphatidylserine expression on erythrocytes and a concomitant loss of viability in vitro.
CD47 ligation has been shown to induce phosphatidylserine (PS) expression as part of a death pathway in nucleated blood cells. Using Annexin V binding assays we showed that ligation of CD47 with the specific CD47-binding peptide 41NK, anti-CD47 monoclonal antibody, and its natural ligand thrombospondin-1 also induced PS expression on enucleated erythrocytes. PS expression was associated with a concomitant loss of erythrocyte viability in vitro. Further characterisation of the CD47-PS signalling pathway on erythrocytes may help develop clinical strategies to further preserve the life of blood donations and improve our understanding of certain types of haemolytic anaemias.
Head
David J
DJ
Genomics Research Institute and Centre for Research in Biomedicine, University of the West of England, Frenchay, Bristol, UK.
Lee
Zoe E
ZE
Swallah
Muhammed M
MM
Avent
Neil D
ND
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000937:Antigen-Antibody Reactions; D015703:Antigens, CD / Q000378:metabolism*; D051928:CD47 Antigen; D002470:Cell Survival; D002478:Cells, Cultured; D004912:Erythrocytes / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D008024:Ligands; D010718:Phosphatidylserines / Q000378:metabolism*; D011485:Protein Binding; D019700:Thrombospondin 1 / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D051928:CD47 Antigen; C497894:CD47 protein, human; D008024:Ligands; D010718:Phosphatidylserines; D019700:Thrombospondin 1