title: Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by use of Chelex 100 resin and polymerase chain reaction.
abstract: A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.
authors.affiliation: State of New York Department of Health, Centers for Molecular Medicine, Stony Brook University, Stony Brook, NY 11794-5120, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Thill
authors.forename: P Bryon
authors.initials: PB
authors.lastname: Backenson
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Prusinski
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Kogut
authors.forename: Joon-Hak
authors.initials: JH
authors.lastname: Lee
authors.forename: James L
authors.initials: JL
authors.lastname: Coleman
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology; D041001:Babesia microti / Q000235:genetics*; D002614:Chelating Agents; D016054:DNA, Protozoan / Q000032:analysis*; D018884:Ixodes / Q000469:parasitology*; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D012117:Resins, Synthetic
chemical_list: D002614:Chelating Agents; D016054:DNA, Protozoan; D012117:Resins, Synthetic; C024997:Chelex 100