Adhesion molecule expression by osteogenic cells cultured on various biodegradable scaffolds.
Design of tissue-engineered cell-loaded device involves cells seeding onto scaffolds in vitro, allowing them to settle and grow before in vivo transplantation. Interaction between scaffold and cells is important in the development of desired tissues. The present study aimed to investigate the effect of cell-polymer interactions on cell morphology and expression of surface markers of osteogenic MBA-15 cells cultured on various bioresorbable polymers. In this study, we used various polymers: poly(L-lactic acid) (PLLA), poly(DL-lactic acid) (PDLLA), poly(L-lactic-glycolic acid) (PLGA), and poly(DL-lactide-glycolide acid) PDLGA1 and PDLGA2. Expression of integrinalpha-M (CD11b), selectin-E (CD62E), and PECAM-1 (CD31), important in cell-cell and cell-matrix interactions, were quantified by flow-cytometry analysis. Cells grown on PDLGA1 films demonstrated fivefold increase in CD62E expression and two-folds increase in CD11b expression. None of the polymers affected the levels of CD31. Identified differential effect of polymers on the expression of cell-adhesion molecules by osteoprogenitors in vitro might help to choose optimal parameters for successful engraftment of cell-loaded constructs.
Shur
I
I
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 69978, Israel.
Zilberman
M
M
Benayahu
D
D
Einav
S
S
D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics*; D018929:Cell Culture Techniques; D002460:Cell Line; D005434:Flow Cytometry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000502:physiology; D017154:Stromal Cells / Q000378:metabolism
D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules