title: Fast separation and sensitive detection of carcinogenic aromatic amines by reversed-phase micro-liquid chromatography coupled with electrochemical detection.
abstract: A micro-LC method was developed for the fast and sensitive analysis of aromatic amines by electrochemical detection. The chromatographic separation of nine carcinogenic aromatic amines was performed on an ABZ + PLUS column with detection limits up to pM L(-1) levels. Mobile phase comprised of methanol-acetate buffer of pH 5 (45:55, v/v) used at a flow rate of 0.2 ml min(-1). The detection was performed with a 6 mm glassy carbon electrode at an applied potential of 0.8 V versus Ag/AgCl. An intraday RSD for retention time and peak area were between 0.22% and 0.73% and 1.86% and 4.03%, respectively. The interdays RSD for retention time and peak area were between 0.47% and 1.35% and 2.04% and 4.42%, respectively. The applicability of the assay has been demonstrated by analyzing these aromatic amines in lake water and synthetic food colour additives. A comparison is given between electrochemical and UV detection.
authors.affiliation: Microfluidics and MEMS Group, Regional Research Laboratory, Bhopal 462 026, India.
authors.forename: Manjusha
authors.initials: M
authors.lastname: Shelke
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Sanghi
authors.forename: Amit
authors.initials: A
authors.lastname: Asthana
authors.forename: Sushma
authors.initials: S
authors.lastname: Lamba
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Sharma
mesh_terms: D000588:Amines / Q000032:analysis / Q000302:isolation & purification*; D002273:Carcinogens / Q000032:analysis / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D005503:Food Additives / Q000737:chemistry; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D000588:Amines; D002273:Carcinogens; D005503:Food Additives; D014874:Water Pollutants, Chemical