[Determination of platelet serotonin by high performance liquid chromatography with fluorometric detector].
OBJECTIVE
To establish a simple, rapid and precise method to determine platelet serotonin.


METHODS
Serotonin (5-hydroxytryptamine; 5-HT) was determined in platelet pellet after simple deproteinization, following ion-pair high performance liquid chromatography (HPLC) on reverse-phase column: Nova-pak C18 column (150 mm x 4.6 mm, 4 microm), serotonin and internal standard (N-methylserotonin) were detected by fluorometry.


RESULTS
The intra-day CV was 2.29% and inter-day CV was 3.88%. The mean recovery of serotonin in platelet was 97.5%, the linear range was 20 - 20000 nmol/ L, and the detection limit was 1 nmol/L. 5-hydroxyindole acetic acid (5-HIAA), Trp, Phe and Tyr had no interference with the chromatographic condition used in this trial.


CONCLUSIONS
The metod described here is simple, convenient, rapid, sensitive, and accurate for clinical and scientific research.
Wang
Qiang
Q
Department of Laboratory Medicine, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Tang
Ai-Guo
AG
Tan
Li-Wen
LW
D000328:Adult; D001792:Blood Platelets / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005470:Fluorometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000097:blood*
D012701:Serotonin