Application of microencapsulated isoflavone into milk.
This study was designed to develop a microencapsulated, water-soluble isoflavone for application into milk and to examine the hypocholesterolemic effect of such a milk product in a rat diet. The coating material was medium-chain triglyceride (MCT) and the core material was water-soluble isoflavone. The microencapsulation efficiency was 70.2% when the ratio (w/w) of coating material to core material was 15:1. The isoflavone release from the microcapsules was 8% after 3-day storage at 40 degrees C. In in vitro study, 4.0-9.3% of water-soluble isoflavone in simulated gastric fluid was released in the pH range of 2 to 5 after 60 min incubation; however, in simulated intestinal fluid at pH 8, 87.6% of isoflavone was released from the capsules after 40 min incubation time. In sensory analysis, the scores of bitterness, astringency, and off-taste in the encapsulated isoflavone-added milk were slightly, but not significantly, different from those in uncapsulated, isoflavone-added milk. In blood analysis, total cholesterol was significantly decreased in the isoflavone-added group compared with that in the control after 6-week feeding. Therefore, this study confirmed the acceptability of MCT as a coating material in the microencapsulation of water-soluble isoflavone for application into milk, although a slight adverse effect was found in terms of sensory attributes. In addition, blood total cholesterol was lowered in rats which had been fed a cholesterol-reduced and microencapsulated, isoflavone-added milk for 6 weeks.
Jeon
Byung-Ju
BJ
Department of Food Science and Technology, Sejong University, 98 Kunja-Dong, Seoul 143-747, Korea.
Kim
Nam-Chul
NC
Han
Eun-Mi
EM
Kwak
Hae-Soo
HS
D000818:Animals; D000924:Anticholesteremic Agents / Q000008:administration & dosage* / Q000737:chemistry; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D018752:Diet, Fat-Restricted; D004339:Drug Compounding; D004355:Drug Stability; D005527:Food, Fortified; D006863:Hydrogen-Ion Concentration; D006937:Hypercholesterolemia / Q000097:blood / Q000178:diet therapy*; D007529:Isoflavones / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D008892:Milk / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature; D014280:Triglycerides / Q000097:blood
D000924:Anticholesteremic Agents; D008076:Cholesterol, HDL; D007529:Isoflavones; D014280:Triglycerides; D002784:Cholesterol