title: High-performance liquid chromatographic assay for the active saponins from Panax notoginseng in rat tissues.
abstract: A reversed-phase liquid chromatographic method was used to determine the ginsenosides Rg1, Rb1 and Rd of Panax notoginseng in rat tissues (kidney, liver, heart, spleen and lung) after the administration of total saponins of P. notoginseng. The tissue samples were treated with solid-phase extraction prior to HPLC. The calibration curves for the three saponins were linear in the given concentration ranges. The intra-day and inter-day assay coefficients in tissues were between 76 and 120% respectively. The recoveries of all the tissues were higher than 70%. This method was applied to evaluate the distribution of the three major saponins of P. notoginseng in rat tissues.
authors.affiliation: School of Pharmaceutical Sciences and Modern Research Center for Traditional Chinese Medicine, Peking University, Beijing, 100083, People's Republic of China.
authors.forename: Lie
authors.initials: L
authors.lastname: Li
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Sheng
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Zhang
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wang
authors.forename: De-An
authors.initials: DA
authors.lastname: Guo
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D036145:Ginsenosides / Q000032:analysis* / Q000493:pharmacokinetics*; D008297:Male; D005894:Panax / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D014018:Tissue Distribution
chemical_list: D036145:Ginsenosides; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1; C049863:ginsenoside Rd