Organization of the CTX prophage in environmental isolates of Vibrio mimicus.
The organization of the CTX prophage in environmental strains of Vibrio mimicus was investigated. Sixteen hundred non-sucrose fermenting vibrios were examined for ctx gene by hybridization. Out of 1,600 isolates, 6 V. mimicus isolates contained ctxA gene. The organization of CTX prophage was determined by RFLP using ctxA probe. The CTX prophage integrated at a single site in V. mimicus genome which was present as a single copy flanked by at least a single RS element. Ribotype pattern revealed that a particular clone of V. mimicus acquired the CTXPhi in the aquatic environment. This study demonstrated that V. mimicus could act as a reservoir of CTXPhi in the aquatic environment.
Islam
Mohammad Sirajul
MS
International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh. sislam@icddrb.org
Rahman
Mohammed Ziaur
MZ
Khan
Sirajul Islam
SI
Mahmud
Zahid Hayat
ZH
Ramamurthy
Thandavarayan
T
Nair
Gopinath Balakrish
GB
Sack
Richard Bradley
RB
Sack
David Allen
DA
D004279:DNA, Viral / Q000032:analysis; D004783:Environmental Microbiology; D039002:Prophages / Q000235:genetics* / Q000302:isolation & purification; D014733:Vibrio / Q000302:isolation & purification / Q000472:pathogenicity / Q000821:virology*
D004279:DNA, Viral