The detection of DNA deamination by electrocatalysis at DNA-modified electrodes.
The method of electrocatalysis based on using a methylene blue (MB) as an electrochemical indicator and ferricyanide ions [Fe(CN)6]3- as an electron acceptor was applied in screening DNA for lesions caused by deamination of nucleobases. The damaged DNA was modeled by short 18-mer oligonucleotides containing the different number of mismatched target bases (uracil instead of cytosine residues). The hybridization capacity of these oligomers with complementary probes (immobilized on gold electrodes or free) was investigated by both electrochemical methods and UV spectroscopy. We have shown that the amplitude of the reduction signal corresponding to ferricyanide ions considerably increases in the presence of MB. This electrocatalytic effect allowed us to detect the changes in electrochemical properties of DNA caused by dU.dG mismatches. Using differential pulse voltammetry and cyclic voltammetry, we showed that the electron transport from the electrode through the double-stranded DNA to MB and then to ferricyanide ions is suppressed by the mismatches in duplex structure. According to UV-monitored melting data, single or multiple wobble dU.dG base pairs destabilize 18-mer DNA duplex by 9-27 degrees C.
Ostatná
V
V
Faculty of Mathematics, Physics and Computer Sciences, Comenius University, Mlynská dolina F1, 842 48 Bratislava, Slovak Republic.
Dolinnaya
N
N
Andreev
S
S
Oretskaya
T
T
Wang
J
J
Hianik
T
T
D002384:Catalysis; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000528:radiation effects; D003641:Deamination; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D014466:Ultraviolet Rays
D004247:DNA