[Effect of TGFbeta1 antisense RNA expression vector on synthesis and secretion of extracellular matrix in cultured human peritoneal mesothelial cells].
OBJECTIVE
To determine the effect of transforming growth factor beta1 (TGFbeta1) antisense RNA on the synthesis and secretion of extracellular matrix in human peritoneal mesothelial cells (HPMC).


METHODS
The recombinant human TGFbeta1 antisense eukaryotic expression vector was transfected into HPMC. Fibronectin (FN) and plasminogen activator inhibitor-1 (PAI-1) were detected by ELISA method. The expression of FN, collagen I (col I) and PAI-1 mRNA were detected by RT-PCR method.


RESULTS
The mRNA of FN, Col I,nd PAI-1 in HPMC were repressed by TGFbeta1 antisense RNA 24 hours after the transfection in comparison with the control group, which decreased 17%, 26%, and 9.6%, respectively. The protein of FN and PAI-1 were inhibited 48 hours after the transfection (P < 0.05).


CONCLUSIONS
Recombinant human TGFbeta1 antisense eukaryotic expression vector can inhibit the synthesis and secretion of extracellular matrix in HPMC which may be effective in gene therapy for peritoneal fibrosis.
Yuan
Fang
F
Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Liu
Fu-you
FY
Liu
Ying-hong
YH
Duan
Shao-bin
SB
Peng
You-ming
YM
Huang
Liang-qun
LQ
D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000378:metabolism; D005109:Extracellular Matrix / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D010537:Peritoneum / Q000166:cytology / Q000378:metabolism*; D016372:RNA, Antisense / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
D016372:RNA, Antisense; D011994:Recombinant Proteins; D053773:Transforming Growth Factor beta1