title: Mechanisms of, and barriers to, horizontal gene transfer between bacteria.
abstract: Bacteria evolve rapidly not only by mutation and rapid multiplication, but also by transfer of DNA, which can result in strains with beneficial mutations from more than one parent. Transformation involves the release of naked DNA followed by uptake and recombination. Homologous recombination and DNA-repair processes normally limit this to DNA from similar bacteria. However, if a gene moves onto a broad-host-range plasmid it might be able to spread without the need for recombination. There are barriers to both these processes but they reduce, rather than prevent, gene acquisition.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. c.m.thomas@bham.ac.uk
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Thomas
authors.forename: Kaare M
authors.initials: KM
authors.lastname: Nielsen
mesh_terms: D001419:Bacteria / Q000235:genetics*; D003227:Conjugation, Genetic / Q000235:genetics; D019143:Evolution, Molecular; D018014:Gene Transfer Techniques; D008957:Models, Genetic; D009154:Mutation; D014169:Transformation, Bacterial / Q000235:genetics