Antiproliferative effect of diallyl disulfide (DADS) on prostate cancer cell line LNCaP.
Garlic has been used throughout the world to treat coughs, toothache, earache, dandruff, hypertension, hysteria, diarrhoea, dysentery, diptheria, vaginitis and many other conditions. Garlic contains a complex mixture of oil and water-soluble organosulfur compounds. Diallyl disulfide (DADS), an oil-soluble constituent of garlic seems to be effective in reducing tumour cells originating from colon, lung and skin. Hence our present study focuses on the dose-dependent effect of DADS on an androgen-dependent prostate cancer cell line. Various concentrations of DADS ranging from 25 to 100 microM were given to LNCaP cells and the activity of lactate dehydrogenase (LDH) prostatic acid phosphatase (PAcP) and the level of prostate specific antigen were studied. DADS reduced the secretory activity of LNCaP cells with the gradual increase in dosage. DADS was found to act as a good antiproliferative agent, which was confirmed by proliferation assay. DADS also induced apoptosis and nuclear segmentation in the higher doses.
Gunadharini
D N
DN
Department of Endocrinology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India.
Arunkumar
A
A
Krishnamoorthy
G
G
Muthuvel
R
R
Vijayababu
M R
MR
Kanagaraj
P
P
Srinivasan
N
N
Aruldhas
M M
MM
Arunakaran
J
J
D000135:Acid Phosphatase; D000498:Allyl Compounds / Q000494:pharmacology*; D000728:Androgens / Q000378:metabolism; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects*; D004220:Disulfides / Q000494:pharmacology*; D005737:Garlic; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D017430:Prostate-Specific Antigen / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000188:drug therapy*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism
D000498:Allyl Compounds; D000728:Androgens; D016588:Anticarcinogenic Agents; D004220:Disulfides; C028009:diallyl disulfide; D007770:L-Lactate Dehydrogenase; D000135:Acid Phosphatase; C428439:prostatic acid phosphatase; D017027:Protein Tyrosine Phosphatases; D017430:Prostate-Specific Antigen