title: Biodegradable and photocrosslinkable polyphosphoester hydrogel.
abstract: A new biodegradable, photocrosslinkable and multifunctional macromer, poly(6-aminohexyl propylene phosphate) (PPE-HA)-ACRL, was synthesized by conjugation of acrylate groups to the side chains of PPE-HA. By controlling the synthetic conditions, different weight fractions of acrylate in the macromers were achieved as confirmed by 1H NMR. The hydrogels obtained from PPE-HA-ACRL through photocrosslinking were dominantly elastic. With increasing acrylate contents in the macromers, the hydrogels exhibited a lower swelling ratio and higher mechanical strength. The hydrogels with different crosslinking densities lost between 4.3% and 37.4% of their mass in 84 days when incubated in phosphate-buffered saline at 37 degrees C. No significant cytotoxicity of the macromers against bone marrow-derived mesenchymal stem cells from goat (GMSC) was observed at a concentration up to 10mg/ml. Finally, GMSCs encapsulated in the photopolymerized gel maintained their viability when cultured in osteogenic medium for three weeks. Clear mineralization in the hydrogel scaffold was revealed by Von Kossa staining. This study suggests the potential of these biodegradable and photocrosslinkable as injectable tissue engineering scaffolds.
authors.affiliation: Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Shilpa
authors.initials: S
authors.lastname: Shahani
authors.forename: Danny D N
authors.initials: DD
authors.lastname: Sun
authors.forename: Blanka
authors.initials: B
authors.lastname: Sharma
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Elisseeff
authors.forename: Kam W
authors.initials: KW
authors.lastname: Leong
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000528:radiation effects; D006041:Goats; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010777:Photochemistry / Q000379:methods; D011126:Polypropylenes / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects; D023822:Tissue Engineering / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020100:Hydrogels; D011126:Polypropylenes; C508217:poly(6-aminohexyl propylene phosphate)