[Construction of co-expression vector pSLC-IRES-IL-2 and its expression in COS-7 cells].
OBJECTIVE
To construct an eukaryotic co-expression plasmid pSLC-IRES-IL-2, and to express it in COS-7 cells.


METHODS
Human IL-2 and SLC genes were cloned by RT-PCR and PCR, respectively, and then the eukaryotic expression plasmid pSLC-IRES-IL-2 was constructed.The constructed plasmid was transfected into COS-7 cells by electroporation method. The expression of SLC and IL-2 was detected by Western blot.


RESULTS
SLC and IL-2 in the culture supernatant and lysate of transfected COS-7 cells were detected by Western blot. The relative molecular masses of the expressed products were consistent with the theoretical values.


CONCLUSIONS
The SLC and IL-2 co-expression plasmid is successfully constructed and expressed in COS-7 cells, which offers a pathway for research on gene therapy of tumors.
Hou
Li
L
Medical Research Center of Shandong Provincial Hospital, Shandong University, Jinan 250021, China. angelhl@126.com
Zhao
Yue-ran
YR
Wang
Lai-cheng
LC
Jiao
Yu-lian
YL
Zhang
Jie
J
Ma
Chun-yan
CY
Cui
Bin
B
D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D054421:Chemokine CCL21 / Q000235:genetics* / Q000378:metabolism*; D002522:Chlorocebus aethiops; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D054421:Chemokine CCL21; D007376:Interleukin-2