[Single strand conformation polymorphism (SSCP) of selected loci of the cps region for capsule synthesis in epidemic and casually isolated strains of Klebsiella pneumoniae in Poland].
The SSCP of ORFs 1, 2, 3, 15 from the cps region for capsule biosynthesis was determined for 56 epidemic isolates, 4 reference strains of K. pneumoniae including A5054 and B5055, and 12 strains isolated casually from stool samples. From 6 up to 14 different SSCP-profiles were observed for tested loci, which combined together distinguished 31 SSCP-genotypes. Epidemic strains could be diversified into 15 genotypes whereas 11 genotypes were detected for 12 casual isolates. Strains from the same outbreak belonged to a single genotype. Strains from different outbreaks represented separate genotypes, however majority of them was located in the same main branch of a dendrogram based on the cluster analysis of the SSCP-profiles diversity. Obtained results may suggest high genetic diversity of tested loci. The SSCP genotyping of multiple cps loci was found as potentially useful tool for tracing epidemic strains during an outbreak.
Gierczyński
Rafał
R
Zakład Bakteriologii PZH w Warszawie. rgierczynski@pzh.gov.pl
Kałuzewski
Stanisław
S
Zasada
Aleksandra A
AA
Rastawicki
Waldemar
W
Jagielski
Marek
M
D016667:Bacterial Capsules / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D005838:Genotype; D007711:Klebsiella pneumoniae / Q000235:genetics*; D011044:Poland; D018807:Polymorphism, Single-Stranded Conformational
D004269:DNA, Bacterial