title: Frequent loss of membranous E-cadherin in gastric cancers: A cross-talk with Wnt in determining the fate of beta-catenin.
abstract: The potential correlation of E-cadherin reduction and Wnt2 up-regulation in determining the intracellular distribution of beta-catenin in gastric cancers was investigated by the methods of frozen tissue array-based immunohistochemistry, Western blot and RT-PCR analysis. It was revealed that membranous E-cadherin was reduced frequently in the two major subtypes of gastric cancer (intestinal gastric cancer, i-GC and diffuse gastric cancer, d-GC) and closely correlated with the risk of lymphoid node metastasis (P < 0.05). The reduction of membranous E-cadherin was paralleled with cytosolic and nuclear accumulation of beta-catenin and the increased Wnt2 expression. These results indicate that the reduced E-cadherin is a common genetic phenotype of GCs and plays beneficial roles in tumor metastasis. Altered beta-catenin distribution may result from the imbalance of E-cadherin production and Wnt expression, which confers on gastric cancer cells more aggressive behaviors.
authors.affiliation: Cancer Institute and The Liaoning Provincial Key Laboratory of Cancer Genomics, College of Basic Medical Sciences, Dalian Medical University, Dalian 116027, P. R. China.
authors.forename: Xiao-Xin
authors.initials: XX
authors.lastname: Cheng
authors.forename: Zi-Chuang
authors.initials: ZC
authors.lastname: Wang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sun
authors.forename: Qing-You
authors.initials: QY
authors.lastname: Kong
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Guan
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D015820:Cadherins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry; D002467:Cell Nucleus / Q000737:chemistry; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000378:metabolism*; D003600:Cytosol / Q000737:chemistry; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008207:Lymphatic Metastasis; D009154:Mutation; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D046888:Tissue Array Analysis; D015534:Trans-Activators / Q000032:analysis / Q000378:metabolism*; D051153:Wnt Proteins; D051154:Wnt2 Protein; D051176:beta Catenin
chemical_list: C495270:CTNNB1 protein, human; D015820:Cadherins; D003598:Cytoskeletal Proteins; D036341:Intercellular Signaling Peptides and Proteins; D015534:Trans-Activators; C495244:WNT2 protein, human; D051153:Wnt Proteins; D051154:Wnt2 Protein; D051176:beta Catenin