title: Chemokine-like factor 1 is a functional ligand for CC chemokine receptor 4 (CCR4).
abstract: Chemokine-like factor 1 (CKLF1) exhibits chemotactic effects on leukocytes. Its amino acid sequence shares similarity with those of TARC/CCL17 and MDC/CCL22, the cognate ligands for CCR4. The chemotactic effects of CKLF1 for CCR4-transfected cells could be desensitized by TARC/CCL17 and markedly inhibited by PTX. CKLF1 induced a calcium flux in CCR4-transfected cells and fully desensitized a subsequent response to TARC/CCL17, and TARC/CCL17 could partly desensitize the response to CKLF1. CKLF1 caused significant receptor internalization in pCCR4-EGFP transfected cells. Taken together, CKLF1 is a novel functional ligand for CCR4.
authors.affiliation: Center for Human Disease Genomics, Peking University, Beijing, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.forename: Wenling
authors.initials: W
authors.lastname: Han
authors.forename: Yanan
authors.initials: Y
authors.lastname: Liu
authors.forename: Qianmei
authors.initials: Q
authors.lastname: Xu
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Chunhui
authors.initials: C
authors.lastname: Di
authors.forename: Quansheng
authors.initials: Q
authors.lastname: Song
authors.forename: Dalong
authors.initials: D
authors.lastname: Ma
mesh_terms: D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D054414:Chemokine CCL17; D018925:Chemokines / Q000494:pharmacology*; D019742:Chemokines, CC / Q000494:pharmacology; D002633:Chemotaxis; D006801:Humans; D008024:Ligands; D062792:MARVEL Domain-Containing Proteins; D054398:Receptors, CCR4; D019707:Receptors, Chemokine / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection
chemical_list: C106230:CCL17 protein, human; C515354:CCR4 protein, human; C432676:CKLF protein, human; D054414:Chemokine CCL17; D018925:Chemokines; D019742:Chemokines, CC; D008024:Ligands; D062792:MARVEL Domain-Containing Proteins; D054398:Receptors, CCR4; D019707:Receptors, Chemokine; D011994:Recombinant Proteins; D002118:Calcium