Detection of T cell memory to measles virus in experimentally infected rhesus macaques by cytokine flow cytometry.
A low, average frequency (0.61%) of measles virus (MV)-specific CD4 and CD8+ T cells was detected in rhesus monkeys experimentally infected with or vaccinated against MV. Both IFN-gamma and TNF-alpha positive T cells were visualized by flow cytometry. However, the conditions of short-term culture and stimulation to detect MV-specific T cells required significant modifications from a previously established method that reliably detects T cells in rhesus monkeys persistently infected with SIV. Both whole viral antigen and short synthetic peptide pools were an adequate antigenic stimulus. MV-specific T cells were detectable up to 11 years after exposure to the virus, although we cannot rule out possible subclinical re-exposure of the monkeys to vaccine virus during this time. Thus, flow cytometric methods can permit mechanistic studies of antigen-specific memory T cell dynamics following an acute viral infection in a primate model.
Pahar
Bapi
B
California National Primate Research Center, University of California-Davis, Davis, California 95616, USA.
Li
Jun
J
McChesney
Michael B
MB
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D007156:Immunologic Memory / Q000276:immunology*; D037181:Lectins, C-Type; D008253:Macaca mulatta; D008297:Male; D008457:Measles / Q000276:immunology / Q000378:metabolism; D008458:Measles Vaccine / Q000008:administration & dosage / Q000276:immunology*; D008459:Measles virus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014764:Viral Proteins / Q000276:immunology; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D016207:Cytokines; D037181:Lectins, C-Type; D008458:Measles Vaccine; D014409:Tumor Necrosis Factor-alpha; D014764:Viral Proteins; D000099192:CD69 Antigens