Mannheimia (Pasteurella) haemolytica leukotoxin binding domain lies within amino acids 1 to 291 of bovine CD18.
Previously, we identified bovine CD18 as the receptor for leukotoxin secreted by Mannheimia (Pasteurella) haemolytica. In this study, we constructed bovine-murine CD18 chimeras to locate the leukotoxin binding domain on CD18. Leukotoxin specifically lysed transfectants expressing bovine CD18 fragment encompassing amino acids 1 to 291, indicating that leukotoxin binding domain lies within amino acids 1 to 291 of bovine CD18.
Gopinath
R S
RS
Department of Veterinary and Biomedical Sciences, University of Nebraska--Lincoln, Lincoln, Nebraska 68583-0905, USA.
Ambagala
T C
TC
Deshpande
M S
MS
Donis
R O
RO
Srikumaran
S
S
D000818:Animals; D018821:CD18 Antigens / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005098:Exotoxins / Q000235:genetics / Q000378:metabolism*; D016978:Mannheimia haemolytica / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
D018821:CD18 Antigens; D018076:DNA, Complementary; D005098:Exotoxins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C018579:leukotoxin