title: Preparative isolation and purification of two phenylbutenoids from the rhizomes of Zingiber cassumunar by upright counter-current chromatography.
abstract: Two phenylbutenoids, (E)-4-(3',4'-dimethoxyphenyl)but-3-enyl acetate and (E)-4-(3',4'-dimethoxyphenyl)but-1,3-diene, were separated from the rhizomes of Zingiber Cassumunar using a preparative upright counter-current chromatography (CCC). With a two-phase solvent system composed of light petroleum (b.p. 60-90 degrees C)-ethanol-diethyl ether-water (5:4:2:1, v/v), 150 mg of (E)-4-(3',4'-dimethoxyphenyl)but-3-enyl acetate and 175 mg of (E)-4-(3','-dimethoxyphenyl)but- 1,3-diene with the purity of 98.7 and 95.1%, respectively, were obtained from 600 mg of the crude sample of Z. Cassumunar in a single-step separation. Structures of these two compounds were identified by ESI-MS, 1H NMR and 13C NMR.
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang Province 310027, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Lu
authors.forename: Cuirong
authors.initials: C
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuanjiang
authors.initials: Y
authors.lastname: Pan
mesh_terms: D000085:Acetates / Q000737:chemistry*; D002073:Butanes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: C504953:4-(3',4'-dimethoxyphenyl)but-3-enyl acetate; D000085:Acetates; D002073:Butanes