title: Production of lysophosphatidic acid in blister fluid: involvement of a lysophospholipase D activity.
abstract: Lysophosphatidic acid (LPA) is present in abundance in serum resulting from platelet activation and is also found in other biological fluids. LPA controls numerous cellular responses and plays a role in specific functions such as wound healing, especially in the skin. Nevertheless, its presence in the skin has never been investigated. Since re-epithelialization occurs after blister rupture, we tested the presence of endogenous LPA in blister fluid and investigated a possible mechanism for its biosynthesis and biological functions. Using a radioenzymatic assay, LPA was detected in 33 blister fluids originating from 24 bullous dermatoses, and at higher concentrations than in plasma. In parallel, blister fluids contained a lysophospholipase D (LPLD) activity but no detectable phospholipase A2 activity. The expressions of the LPLD autotaxin (ATX) and of LPA1-receptor (LPA1-R) were greatly increased in blister skin when compared with normal skin. Finally, LPA was found to have a positive effect on the migration of cultured keratinocytes. These results show that LPA is present in blister fluid synthesized by the LPLD ATX. Due to its ability to enhance keratinocyte migration, LPA in blister fluid could, via the LPA1-R, play an important role in re-epithelialization occurring after blister rupture.
authors.affiliation: Service de Dermatologie, Hôpital Rangueil, Toulouse Cedex, France. mazereeuw-hautier.j@chu-toulouse.fr
authors.forename: Juliette
authors.initials: J
authors.lastname: Mazereeuw-Hautier
authors.forename: Sandra
authors.initials: S
authors.lastname: Gres
authors.forename: Madie
authors.initials: M
authors.lastname: Fanguin
authors.forename: Clotilde
authors.initials: C
authors.lastname: Cariven
authors.forename: Josette
authors.initials: J
authors.lastname: Fauvel
authors.forename: Bertrand
authors.initials: B
authors.lastname: Perret
authors.forename: Hugues
authors.initials: H
authors.lastname: Chap
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Salles
authors.forename: Jean-Sébastien
authors.initials: JS
authors.lastname: Saulnier-Blache
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001768:Blister / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement; D002478:Cells, Cultured; D005260:Female; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D008246:Lysophospholipids / Q000032:analysis / Q000096:biosynthesis* / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011755:Pyrophosphatases; D049368:Receptors, Lysophosphatidic Acid / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D014945:Wound Healing; D000099189:Lysophospholipase D
chemical_list: D005956:Glucose-6-Phosphate Isomerase; D006023:Glycoproteins; D008246:Lysophospholipids; D009097:Multienzyme Complexes; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D049368:Receptors, Lysophosphatidic Acid; D000099189:Lysophospholipase D; C032881:lysophosphatidic acid