[Expression of vascular endothelial growth factor and thrombospondin-1 in the ectopic and eutopic endometrium of women with endometriosis].
OBJECTIVE
To detect the expression of vascular endothelial growth factor (VEGF) and thrombospondin (TSP)-1 mRNA in the ectopic and eutopic endometrium of patients with endometriosis.


METHODS
Thirty-two specimens of ovarian endometrioma, 12 red peritoneal lesion, 8 uterosacral ligament nodule, and 30 matched eutopic endometrium were biopsied from 38 patients with endometriosis, another 25 specimens of endometrium were biopsied from women without endometriosis. Reverse transcript-polymerase chain reaction (RT-PCR) and Northern Blot analysis were utilized to examine VEGF mRNA and TSP-1 mRNA expression and their relative levels in the above endometrium.


RESULTS
Among endometriotic lesions, red peritoneal lesion expressed higher level of VEGF and lower level of TSP-1, whereas ovarian endometrioma expressed lower level VEGF and higher level of TSP-1. Eutopic endometrium of women with endometriosis had higer level of VEGF expression and lower level of TSP-1 expression than women without endometriosis.


CONCLUSIONS
The expression of VEGF and TSP-1 in endometriotic lesion appears to be associated with its neovalscualization extent. The imbalance in expression of VEGF and TSP-1 in endometrium might be related to the development of endometriosis and its biological behavior.
Tan
X
X
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Lang
J
J
Liu
D
D
D015152:Blotting, Northern; D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D010049:Ovarian Diseases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019700:Thrombospondin 1 / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D019700:Thrombospondin 1; D042461:Vascular Endothelial Growth Factor A