Formation of polyelectrolyte multilayer architectures with embedded DMPC studied in situ by neutron reflectometry.
The coupling of lipid molecules to polymer components in a planar biomimetic model membrane made of a lipid bilayer (dimyristoylphosphatidylcholine) supported by polyelectrolyte multilayers is studied. The polyelectrolyte support was prepared by layer-by-layer deposition of positively charged poly(allylamine hydrochloride) (PAH) and negatively charged poly(sodium 4-styrenesulfonate) (PSS). Two polymer sample terminations were considered: positively charged (PAH-terminated) and negatively charged (PSS-terminated). Neutron reflectometry studies showed that, whereas positively charged samples did not favor the deposition of lipid, negatively charged samples allowed the deposition of a lipid bilayer with a thickness of approximately 5 nm. In the latter case, formation of polyelectrolyte layers after the deposition of the lipid layer was also possible.
Delajon
Christophe
C
Max-Planck Institute of Colloids and Interfaces, Wissenschaftspark Golm, 14424 Potsdam, Germany.
Gutberlet
Thomas
T
Steitz
Roland
R
Möhwald
Helmuth
H
Krastev
Rumen
R
D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D015394:Molecular Structure; D009502:Neutrons
D004573:Electrolytes; D004134:Dimyristoylphosphatidylcholine