title: [Effects of A23187 on intracellular free Ca2+ concentration and apoptosis on human squamous cell carcinoma of larynx].
abstract: OBJECTIVE
  To study the effects of A23187 on intracellular free Ca2+ concentration and apoptosis on human squamous cell carcinoma of larynx.
  
  
  METHODS
  Human squamous cell carcinoma of larynx were randomly divided into 4 groups ( 3 treated with A23187 groups and 1 control). Intracellular free Ca2+ was measured by using the fluorescence Ca2+ indicator Fura-2/AM; Apoptotic rate was measured by using FACS 420 Flow Cytometer; DNA gel electrophoresis at different time points.
  
  
  RESULTS
  Intracellular [Ca2+]i at 5 h, 24 h, 48 h after A23187 exposure were (123.48+/-21.54) mmol/L, (110.54+/-11.32) mmol/L, (88.63+/-9.95) mmol/L and all were higher than control group (54.23+/-8.73) nmol/L (P <0.01); Apoptotic rate in 5, 24, 48 h were (3.84+/-1.12), (7.65+/-1.35), (9.49+/-1.31) and also higher than control group (2.63+/-0.75) (P < 0.01) and increased with time prolonged.
  
  
  CONCLUSIONS
  Exposuring to A23187 would interfere with intracellular free Ca2+ concentration and apoptotic rate in human squamous cell carcinoma of larynx suggests that there may be certain relation between them.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the Third Affiliated Hospital of Sun-Yat-Sen University, Guangzhou, 510630, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Zhang
authors.forename: Weijia
authors.initials: W
authors.lastname: Kong
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000032:analysis*; D045744:Cell Line, Tumor; D006801:Humans; D007476:Ionophores / Q000494:pharmacology*; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*
chemical_list: D007476:Ionophores; D000001:Calcimycin; D002118:Calcium