Different expression patterns of duplicated PHANTASTICA-like genes in Lotus japonicus suggest their divergent functions during compound leaf development.
Recent studies on leaf development demonstrate that the mechanism on the adaxial-abaxial polarity pattern formation could be well conserved among the far-related species, in which PHANTASTICA (PAHN)-like genes play important roles. In this study, we explored the conservation and diversity on functions of PHAN-like genes during the compound leaf development in Lotus japonicus, a papilionoid legume. Two PHAN-like genes in L. japonicus, LjPHANa and LjPHANb, were found to originate from a gene duplication event and displayed different expression patterns during compound leaf development. Two mutants, reduced leaflets1 (rel1) and reduced leaflets3 (rel3), which exhibited decreased adaxial identity of leaflets and reduced leaflet initiation, were identified and investigated. The expression patterns of both LjPHANs in rel mutants were altered and correlated with abnormalities of compound leaves. Our data suggest that LjPHANa and LjPHANb play important but divergent roles in regulating adaxial-abaxial polarity of compound leaves in L. japonicus.
Luo
Jiang Hong
JH
National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Shanghai 200032, China.
Yan
Jun
J
Weng
Lin
L
Yang
Jun
J
Zhao
Zhong
Z
Chen
Jiang Hua
JH
Hu
Xiao He
XH
Luo
Da
D
D000595:Amino Acid Sequence; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D020131:Genes, Duplicate / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000070116:Lotus / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D017385:Sequence Homology
D018398:Homeodomain Proteins; C405985:Knox1 protein, plant; D010940:Plant Proteins