Can p503s, p504s and p510s gene expression in peripheral-blood be useful as a marker of prostatic cancer?
BACKGROUND
The aim of the study was to investigate whether p503S, p504S and p510S gene expression in peripheral-blood be useful as a diagnostic or prognostic marker of prostatic cancer.


METHODS
Circulating cells were identified by reverse transcription-polymerase chain reaction (RT-PCR) to detect p503S, p504S and p510S mRNA in peripheral blood (PB) from 11 patients with treated prostatic carcinoma (CaP), 11 with newly-diagnosed untreated CaP and 20 with benign prostatic hyperplasia (BPH) (controls).


RESULTS
RT-PCR amplified P503S in 7 of 11 untreated and 2 of 11 treated patients with CaP and 5 of 20 with BPH; p504S in 7 of 11 untreated and in 9 of 11 treated patients with CaP and 11 of 20 with BPH; whereas it amplified p510S in all subjects with CaP and in 15 of 20 with BPH.


CONCLUSIONS
These findings suggest that the investigated genes are poorly specific and probably of little use as diagnostic or prognostic prostatic markers in peripheral blood for monitoring disease progression and recurrence.
Cardillo
Maria Rosaria
MR
Department of Experimental Medicine and Pathology, University La Sapienza, Viale Regina Elena, 161 Rome, Italy. mariarosaria.cardillo@uniroma1.it
Gentile
Vincenzo
V
Ceccariello
Antonio
A
Giacomelli
Laura
L
Messinetti
Stefano
S
Di Silverio
Franco
F
D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D014408:Biomarkers, Tumor; D018076:DNA, Complementary / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D008875:Middle Aged; D008969:Molecular Sequence Data; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000097:blood*; D020411:Oligonucleotide Array Sequence Analysis; D011470:Prostatic Hyperplasia / Q000097:blood* / Q000175:diagnosis*; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis*; D012313:RNA / Q000378:metabolism; D019998:Racemases and Epimerases / Q000097:blood*; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059470:Tetraspanins
D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D008565:Membrane Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012313:RNA; D019998:Racemases and Epimerases; D059470:Tetraspanins; C073492:ABCC4 protein, human; C508125:TSPAN1 protein, human; C108352:alpha-methylacyl-CoA racemase