HIV-1 Cis Enhancing Sequence (CES) enhances CTE-dependent Gag expression.
In order to export intron-containing RNA from nucleus, retroviruses use either viral trans-acting factors or constitutive cellular factors interacting with cis-elements in their intron-containing RNA. We have previously identified a Cis Enhancing Sequence (CES) in HIV-1 env region that could co-operate with Rev and RRE to enhance Gag expression by promoting RNA stabilization and exportation. In this study, we found that CES could function in a Rev-independent manner by co-operating with a Constitutive Transport Element (CTE) of Mason-Pfizer monkey viruses (MPMV). RRE and CTE promote intron-containing RNA exportation through different pathways. The fact that CES could function in both pathways of RNA export suggested that CES might function at a common step either up- or downstream to Rev/RRE or CTE functions. Known hnRNP-A1-binding sites as well as other 3 highly conserved sequences in the CES were found to be required for its activity.
Suptawiwat
Ornpreya
O
Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Lee
Tun-Hou
TH
Auewarakul
Prasert
P
D000818:Animals; D001483:Base Sequence; D001693:Biological Transport, Active; D019556:COS Cells; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D015870:Gene Expression; D015750:Genes, gag; D005816:Genetic Complementation Test; D014644:Genetic Variation; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007438:Introns; D016093:Mason-Pfizer monkey virus / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D012333:RNA, Messenger; D012367:RNA, Viral