[Effect of jiuxinfumai injection on L-type calcium currents in single guinea pig ventricular myocytes].
OBJECTIVE
To determine the effect of 311 and 417, both active ingredients isolated from Jiuxinfumai injection (Citrus Aurantium) on L-type calcium currents (I(Ca-L)) in ventricular myocytes of guinea pigs.


METHODS
Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of 311and 417.


RESULTS
311 (10, 25, 50, 100 mmol/L) increased the (I(Ca-L)) by 8.27%, 27.29%, 41.01%, and 48.74% (P < 0.05), respectively. 417 (10, 25, 50, 100 mmol/L) increased the (I(Ca-L)) by 10.05%, 30.12%, 43.05%, and 51.90% (P < 0.05), respectively. Both 311 and 417 changed the (I(Ca-L)) significantly in a concentration-dependent manner. They did not change the shape of I-V cruves.


CONCLUSIONS
311 and 417 can increase I(I(Ca-L)) n ventricular myocytes of guinea pigs in a dose-response manner.
Fang
Fang
F
School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China.
Song
Tao
T
Xie
Li-xin
LX
Liang
Song-ping
SP
Liu
Mei-li
ML
Zhou
Hong-hao
HH
Fang
Yun-xiang
YX
D000818:Animals; D020746:Calcium Channels, L-Type / Q000187:drug effects* / Q000502:physiology; D002957:Citrus / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000166:cytology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques
D020746:Calcium Channels, L-Type; D004365:Drugs, Chinese Herbal