Probing leukocyte traffic in lymph from oro-nasal mucosae by cervical catheterization in a sheep model.
Lymph nodes are instructed via the lymph about ongoing events in tissues both during the steady state and under provoked inflammation. In order to probe for tissue-to-node transduction mechanisms, we have developed a novel in vivo technique of pseudo-afferent lymph collection from the oro-nasal mucosae which represent the main portals of entry of micro-organisms and efficient routes for vaccination. After lateral lymph node resection of the head, a network of lymph ducts was reconstructed as checked by lymphography. Subsequent catheterization of the cervical lymph duct allowed the collection of cells that were shown to originate from the oro-nasal mucosae. These cells included dendritic cells, monocytes, granulocytes, memory CD45RAneg CD2pos integrin beta7lo CD4 T cells, CD25pos CD4, CD8, gamma/delta T cells, and B lymphocytes. This approach, which permits lymph collection over several weeks, opens a valuable and unique way to study leukocyte and particulate (micro-organisms, vaccines) trafficking from head tissue to nodes under homeostastic and immuno-stimulatory conditions in a highly physiological setting.
Schwartz-Cornil
Isabelle
I
Virologie et Immunologie Moléculaires UR892 INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France. isabelle.schwartz@jouy.inra.fr
Epardaud
Mathieu
M
Albert
Jean-Pierre
JP
Bourgeois
Christian
C
Gérard
Franck
F
Raoult
Isabelle
I
Bonneau
Michel
M
D000818:Animals; D002404:Catheterization; D002465:Cell Movement / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D016130:Immunophenotyping; D007962:Leukocytes / Q000166:cytology / Q000276:immunology*; D008196:Lymph / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology* / Q000601:surgery; D023421:Models, Animal; D009297:Nasal Mucosa / Q000276:immunology*; D012756:Sheep / Q000276:immunology* / Q000601:surgery