title: Characterization of 26 isolates of Staphylococcus aureus, predominantly from dairy sheep, using four different techniques of molecular epidemiology.
abstract: Little information is available regarding the molecular epidemiology of Staphylococcus aureus-induced mastitis in dairy sheep. In this study, 4 different typing techniques were compared in typing 26 S. aureus isolates, predominantly from cases of subclinical mastitis in dairy ewes. The 4 techniques were pulsed-field gel electrophoresis (PFGE), restriction fragment length polymorphism (RFLP) on 2 genes (coagulase and clumping factor B), randomly amplified polymorphic DNA-polymerase chain reaction (PCR) (RAPD-PCR), and multilocus sequence typing (MLST). On the basis of discriminatory power as the key parameter of typing systems, MLST and PFGE were found to be the most powerful techniques. The MLST and PFGE could contribute to epidemiological surveillance and evaluation of mastitis control programs, by documenting prevalence and dissemination of endemic clones in infected populations. The results of this study show that a single clone of S. aureus is widely distributed in infected ewe mammary glands.
authors.affiliation: Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches sur les Petits Ruminants et les Abeilles, BP 111, F-06902 Sophia Antipolis, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Vautor
authors.forename: Corinne
authors.initials: C
authors.lastname: Jay
authors.forename: Nicolas
authors.initials: N
authors.lastname: Chevalier
authors.forename: Nathalie
authors.initials: N
authors.lastname: Visomblin
authors.forename: Guy
authors.initials: G
authors.lastname: Vernet
authors.forename: Michel
authors.initials: M
authors.lastname: Pépin
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods / Q000662:veterinary*; D003030:Coagulase / Q000235:genetics; D003612:Dairying; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods / Q000662:veterinary; D005260:Female; D008413:Mastitis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000382:microbiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics
chemical_list: D003030:Coagulase