title: Chlamydia trachomatis variant with nonfusing inclusions: growth dynamic and host-cell transcriptional response.
abstract: We compared growth rate and host-cell transcriptional responses of a Chlamydia trachomatis variant strain and a prototype strain. Growth dynamics were estimated by 16S rRNA level and by inclusion-forming units (IFUs) at different times after infection in HeLa cells. When inoculated at the same multiplicity of infection and observed 24-48 h after infection, the variant 16S rRNA transcriptional level was 3%-4% that of the prototype, and the IFUs of the variant strain were 0.1%-1% those of the prototype. Specific host-cell transcriptional responses to the variant were identified in a global-expression microarray in which variant strain-infected cells were compared with mock-infected and prototype strain-infected cells. In variant strain-infected cells, 47% (16/34) of specifically induced host genes were related to immunity and 32% (8/25) of specifically suppressed genes were related to lipid metabolism. The variant strain grew significantly more slowly and induced a modified host-cell transcriptional response, compared with the prototype strain.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University of Washington, Seattle, USA.
authors.forename: Minsheng
authors.initials: M
authors.lastname: Xia
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Suchland
authors.forename: Roger E
authors.initials: RE
authors.lastname: Bumgarner
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Daniel D
authors.initials: DD
authors.lastname: Rockey
authors.forename: Walter E
authors.initials: WE
authors.lastname: Stamm
mesh_terms: D002692:Chlamydia trachomatis / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D005786:Gene Expression Regulation; D014644:Genetic Variation; D006367:HeLa Cells / Q000276:immunology / Q000382:microbiology; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis; D008561:Membrane Fusion; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D008055:Lipids; D011506:Proteins; D012336:RNA, Ribosomal, 16S