title: Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo.
abstract: Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank.
authors.affiliation: Laboratório de Rickettsias, Instituto Adolfo Lutz, São Paulo, SP, Brazil.
authors.forename: Elvira Maria Mendes
authors.initials: EM
authors.lastname: Nascimento
authors.forename: Flávia de Sousa
authors.initials: Fde S
authors.lastname: Gehrke
authors.forename: Rosa Amélia
authors.initials: RA
authors.lastname: Maldonado
authors.forename: Silvia
authors.initials: S
authors.lastname: Colombo
authors.forename: Luiz Jacintho da
authors.initials: LJ
authors.lastname: Silva
authors.forename: Teresinha Tizu Sato
authors.initials: TT
authors.lastname: Schumaker
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D001938:Brazil; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D004269:DNA, Bacterial / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D019353:Endemic Diseases; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012282:Rickettsia Infections / Q000175:diagnosis* / Q000821:virology; D012284:Rickettsia rickettsii / Q000235:genetics*; D013987:Ticks / Q000382:microbiology; D014709:Vero Cells
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial