title: [Isolation and properties of the protein C activator from Agkistrodon halys halys venom].
abstract: Protein C activator from Agkistrodon halys halys venom has been purified by ion-exchange and gel-filtration chromatography. The purified enzyme consists of a single peptide chain with molecular weight of 34 kD. Its pH-optimum was the range of 7,5-8,0. The enzyme was inhibited by DFP, benzamidine, PMSF, EGTA. Protein C activator was as effective as Protac (Pentapharm AG, Switzerland) for determination of protein C level in blood plasma using APTT test and protein C chromogenic substrate.
authors.forename: O V
authors.initials: OV
authors.lastname: Gornitskaia
authors.forename: T N
authors.initials: TN
authors.lastname: Platonova
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D003435:Crotalid Venoms / Q000737:chemistry*; D004211:Disseminated Intravascular Coagulation / Q000097:blood / Q000175:diagnosis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D036341:Intercellular Signaling Peptides and Proteins; D010314:Partial Thromboplastin Time; D010455:Peptides / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D011486:Protein C / Q000032:analysis*
chemical_list: C031442:Agkistrodon venoms; D003435:Crotalid Venoms; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011486:Protein C; C049647:snake venom protein C activator