Detection of antibodies to opioid and glutamate receptors by latex agglutination and enzyme immunoassay.
We studied adsorption capacity of 5 latexes to synthetic peptide fragments of mu- and delta-opioid receptors and to GluR1 and NR2A subunits of glutamate receptor. Levels of autoantibodies to opioid receptors in the latex agglutination test and enzyme immunoassay were in good correlation. The level of autoantibodies to opioid receptors measured by these methods was increased in patients with opium narcomania, while the content of autoantibodies to the glutamate receptor subunits was increased in epileptics.
Kharitonova
A V
AV
Institute of High Molecular Compounds, Russian Academy of Sciences, St. Petersburg, Russia. akharitonova@front.ru
Menshikova
A Yu
AY
Evseeva
T G
TG
Chekina
N A
NA
Bychkov
E R
ER
Skulyabin
D I
DI
Dambinova
S A
SA
D001323:Autoantibodies / Q000097:blood*; D004827:Epilepsy / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007840:Latex / Q000737:chemistry / Q000276:immunology; D007841:Latex Fixation Tests; D009293:Opioid-Related Disorders / Q000175:diagnosis*; D018091:Receptors, AMPA / Q000276:immunology*; D016194:Receptors, N-Methyl-D-Aspartate / Q000276:immunology*; D011957:Receptors, Opioid / Q000276:immunology*
D001323:Autoantibodies; D007840:Latex; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; D011957:Receptors, Opioid; C120997:NR2A NMDA receptor