[Augmentation of revascularization by implantation of autologous bone marrow mononuclear cells in a rat ischemic hind limb model].
OBJECTIVE
To examine whether the in vivo implantation of autologous bone-marrow mononuclear cells (BM-MNCs) can augment postnatal neovascularization in rat model of hind limb ischemia.


METHODS
Rat BM-MNCs were isolated by centrifugation through a Ficoll-paque density gradient. The rat ischemic hind limb model was made by ligation of the right femoral artery and its branches of imbred Wistar rats. BM-MNCs (MNC group) or phosphate saline buffer (PBS group) were injected into 7 points of the ischemic muscles (20 microl/point). To assess angiogenesis, histologic evaluation was performed on tissue slices from adductor muscles with immunohistochemical staining at the 2nd and 4th weeks after the ligation. Severity of ischemic insult was evaluated by the femoral arteriovenous oxgen difference (AVDO2) at the 2nd and 4th weeks after the ligation.


RESULTS
The MNC groups had a higher capillary density and higher capillary/muscle fiber ratios than that of the sham and PBS groups (P < 0.01) at the 2nd and 4th weeks after the ligation. AVDO2 of the MNC group significantly decreased compared with that of the PBS groups (P < 0.05).


CONCLUSIONS
The implantation of autologous BM-MNCs induced neovascularization in a rat ischemic hind limb model. The implantation of autologous BM-MNCs augmented the collateral perfusion of ischemic hind limb.
Liu
Wen-liang
WL
Department of Thoracic and Cardiovascular Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China. liuwenliang1859@hotmail.com
Yin
Bang-liang
BL
Yu
Feng-lei
FL
Hu
Ye-rong
YR
Chen
Xiao-feng
XF
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000379:methods; D002478:Cells, Cultured; D007511:Ischemia / Q000601:surgery*; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D035002:Lower Extremity / Q000098:blood supply*; D008297:Male; D018919:Neovascularization, Physiologic; D011897:Random Allocation; D051381:Rats; D014182:Transplantation, Autologous