title: Evaluation of normal ocular bacterial flora with two different culture media.
abstract: BACKGROUND
  The purpose of this study was to determine if the use of broth culture medium is efficient in investigating bacterial flora of the normal eyelid and conjunctiva.
  
  
  METHODS
  Samples from the conjunctiva and eyelid of healthy patients of various ages who were undergoing ocular surgeries were obtained and cultured at 3 periods: before topical antibiotic prophylaxis, in the postoperative period during topical antibiotic treatment, and 15 days after discontinuation of antibiotic use. Samples were inoculated into both brain heart infusion broth and blood agar plate, and the growth results of both media were analyzed.
  
  
  RESULTS
  Brain heart infusion broth medium showed a significantly higher bacterial growth of gram-positive cocci in most periods. The solid blood agar medium had a higher recovery of gram-positive bacilli before prophylaxis only in the older patients.
  
  
  CONCLUSIONS
  Our results show that a more complete analysis of eyelid and conjunctival flora can be obtained using both liquid and solid media to increase the chances of isolate recovery. The inclusion of liquid media in this analysis was even more relevant in the period of concomitant use of antibiotic treatment.
authors.affiliation: Ophthalmology Department, Paulista School of Medicine, Federal University of São Paulo, Brazil. ceciliamoeller@uol.com.br
authors.forename: Cecília Tobias de Aguiar
authors.initials: CT
authors.lastname: Moeller
authors.forename: Bruno Castelo
authors.initials: BC
authors.lastname: Branco
authors.forename: Maria Cecília Zorat
authors.initials: MC
authors.lastname: Yu
authors.forename: Michel Eid
authors.initials: ME
authors.lastname: Farah
authors.forename: Manoel A A
authors.initials: MA
authors.lastname: Santos
authors.forename: Ana Luisa
authors.initials: AL
authors.lastname: Höfling-Lima
mesh_terms: D000328:Adult; D000368:Aged; D001419:Bacteria / Q000254:growth & development* / Q000302:isolation & purification*; D003228:Conjunctiva / Q000382:microbiology*; D003470:Culture Media; D005143:Eyelids / Q000382:microbiology*; D006801:Humans; D020731:Keratomileusis, Laser In Situ; D054018:Lasers, Excimer; D018918:Phacoemulsification; D018903:Photorefractive Keratectomy
chemical_list: D003470:Culture Media