Modelling of ceramide interactions with porous graphite carbon in non-aqueous liquid chromatography.
Interactions of solutes on porous graphitic carbon (PGC) with non-aqueous mobile phases are studied by the linear solvation energy relationship (LSER). Studies have been carried out with eight binary mixtures composed of a weak solvent (acetonitrile or methanol) and a strong solvent (tetrahydrofuran, n-butanol, CH2Cl2, 1,1,2-trichloro-2,2,1-trifluoroethane). The systematic analysis of a set of test compounds was performed for each solvent mixture in isocratic mode (50:50). The results were compared to those obtained on PGC with hydro-organic liquids and supercritical fluids. They were then correlated with the observed retention behaviour of lipid compounds, more particularly ceramides.
West
C
C
LETIAM, University de Paris-Sud (XI), IUT d'Orsay, Plateau du Moulon, F-91400 Orsay, France.
Cilpa
G
G
Gaudin
K
K
Chaminade
P
P
Lesellier
E
E
D002518:Ceramides / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006108:Graphite / Q000737:chemistry*
D002518:Ceramides; D006108:Graphite