title: Structure of yeast 5-aminolaevulinic acid dehydratase complexed with the inhibitor 5-hydroxylaevulinic acid.
abstract: The X-ray structure of the enzyme 5-aminolaevulinic acid dehydratase (ALAD) from yeast complexed with the competitive inhibitor 5-hydroxylaevulinic acid has been determined at a resolution of 1.9 A. The structure shows that the inhibitor is bound by a Schiff-base link to one of the invariant active-site lysine residues (Lys263). The inhibitor appears to bind in two well defined conformations and the interactions made by it suggest that it is a very close analogue of the substrate 5-aminolaevulinic acid (ALA).
authors.affiliation: School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, England.
authors.forename: P T
authors.initials: PT
authors.lastname: Erskine
authors.forename: L
authors.initials: L
authors.lastname: Coates
authors.forename: R
authors.initials: R
authors.lastname: Newbold
authors.forename: A A
authors.initials: AA
authors.lastname: Brindley
authors.forename: F
authors.initials: F
authors.lastname: Stauffer
authors.forename: G D E
authors.initials: GD
authors.lastname: Beaven
authors.forename: R
authors.initials: R
authors.lastname: Gill
authors.forename: A
authors.initials: A
authors.lastname: Coker
authors.forename: S P
authors.initials: SP
authors.lastname: Wood
authors.forename: M J
authors.initials: MJ
authors.lastname: Warren
authors.forename: P M
authors.initials: PM
authors.lastname: Shoolingin-Jordan
authors.forename: R
authors.initials: R
authors.lastname: Neier
authors.forename: J B
authors.initials: JB
authors.lastname: Cooper
mesh_terms: D000622:Aminolevulinic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D012545:Schiff Bases
chemical_list: C505712:5-hydroxylaevulinic acid; D005656:Fungal Proteins; D012545:Schiff Bases; D000622:Aminolevulinic Acid; D000623:Porphobilinogen Synthase