title: [PCR detection of transgenic elements in feed raw material].
abstract: Based on the heterogeneous genes usually used in transgenic crops, the PCR technique was performed with primers derived from CaMV 35S promoter (35S-promoter,originated from cauliflower mosaic virus), NOS terminator (nopaline synthase-terminator,derived from Agrobacterium tumefaciens), EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene, and CryIA(b) (delta-endotoxin,evolved from Bacillus thuringiensis subsp. kurstaki) gene to detect transgenic agents from feed raw materials of soybean dregs and corn gluten meal, respectively. Endogenous corn Zein (a protein extracted from corn gluten) gene, soybean Lectin (chitin-binding protein) gene and negative, positive control were applied for avoiding false results. The method established here has been successfully applied in detecting transgenic elements in imported feed raw material.
authors.affiliation: Shenzhen Exit-Entry Inspection and Quarantine Bureau, Shenzhen 518100, China. wy-liza@21cn.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Dao-Jian
authors.initials: DJ
authors.lastname: Yu
authors.forename: Lin
authors.initials: L
authors.lastname: Kang
authors.forename: Gui-Ming
authors.initials: GM
authors.lastname: Zhang
authors.forename: Xian-Zhong
authors.initials: XZ
authors.lastname: Jin
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Yang
authors.forename: Pei-Qing
authors.initials: PQ
authors.lastname: Huang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Zhi-Nan
authors.initials: ZN
authors.lastname: Chen
authors.forename: Cheng-Cai
authors.initials: CC
authors.lastname: Chu
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Cheng