Glucose-6-phosphate dehydrogenase (G6PD) mutations in Cambodia: G6PD Viangchan (871G>A) is the most common variant in the Cambodian population.
We conducted a survey of malaria diagnoses and glucose-6-phosphate dehydrogenase (G6PD) testing in remote areas of Cambodia. Blood specimens from 670 people were collected by the finger-prick method. Of these people, 24.9% were found to have malaria, and 7.0% of people were G6PD deficient. In the Khmer, the largest ethnical population in Cambodia, the G6PD deficiency rate of males was 12.6% (25/199) whereas the rates in the minorities of the Tum Pun and the Cha Ray were 1.1% (1/93) and 3.2% (2/63), respectively. Of the G6PD-deficient subjects, 97.9% (46/47) were G6PD Viangchan (871G>A), and only one case (2.1%) was G6PD Union (1360C>T). Since G6PD Mahidol (487G>A) is common in Myanmar according to our previous study, the current finding suggests that the Cambodian population is derived from homogeneous ancestries and is different from the Myanmar population. All G6PD Viangchan cases were linked to two other mutations of 1311C>T and IVS-11 nt93T>C in the G6PD gene.
Matsuoka
Hiroyuki
H
Department of Medical Zoology, Jichi Medical School, 3311-1 Yakushij, Minami-kawachi, Tochigi, 329-0498, Japan. hiroyuki@jichi.ac.jp.
Nguon
Chea
C
National Malaria Center, Ministry of Health, Phnom Penh, Cambodia.
Kanbe
Toshio
T
Department of Advanced Medical Science, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Jalloh
Amadu
A
Department of Advanced Medical Science, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Sato
Hiroko
H
Faculty of Medicine, Department of Infectious Disease Control, Oita University, Oita, Japan.
Yoshida
Shigeto
S
Department of Medical Zoology, Jichi Medical School, 3311-1 Yakushij, Minami-kawachi, Tochigi, 329-0498, Japan.
Hirai
Makoto
M
Department of Medical Zoology, Jichi Medical School, 3311-1 Yakushij, Minami-kawachi, Tochigi, 329-0498, Japan.
Arai
Meiji
M
Department of Medical Zoology, Jichi Medical School, 3311-1 Yakushij, Minami-kawachi, Tochigi, 329-0498, Japan.
Socheat
Duong
D
National Malaria Center, Ministry of Health, Phnom Penh, Cambodia.
Kawamoto
Fumihiko
F
Faculty of Medicine, Institute of Scientific Research, Oita University, Oita, Japan.
D002160:Cambodia / Q000453:epidemiology; D004252:DNA Mutational Analysis; D017931:DNA Primers; D005006:Ethnicity / Q000235:genetics*; D005820:Genetic Testing; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000453:epidemiology* / Q000235:genetics; D006801:Humans; D008288:Malaria / Q000188:drug therapy / Q000453:epidemiology; D008297:Male; D009154:Mutation / Q000235:genetics*; D015995:Prevalence; D011319:Primaquine / Q000009:adverse effects / Q000627:therapeutic use; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D005954:Glucosephosphate Dehydrogenase; D011319:Primaquine