Tissue distribution of a plasmid DNA containing epitopes of foot-and-mouth disease virus in mice.
It is known that only the minority of plasmid DNAs effect a cure or prevention after intramuscular injection into host. But what is the fate of the majority? And indeed how many of the injected DNAs work? Till now, little is known about it. To answer these questions, two methods including PCR and autoradiography were used in distribution study in mice that had received a single muscular inoculation of plasmid DNA containing antigenic epitopes of foot-and-mouth disease virus. The results showed that the plasmid DNAs were distributed by blood circulation and degraded soon. The degradation ratio of super coiled plasmid DNA was 20.9% in 10 min, 34.1% in 1h, 86.8% in 1 day and 97.8% in 1 week in sera in vivo. And over a half of the whole were output in urine and faeces. The rest resided most in muscles as 'antigen pool', next in immune organs, kidney, liver, heart, lung and little in brain or gonad. About 40% or 0.5% of total plasmid DNAs, inferring to be effective, resided in muscles or immune organs, respectively. Collective results suggested that 'nude' DNA, as water injection, was characterized as quick absorbent, extensive distribution, but low utilization rate. Finally, the immune mechanism for the DNA vaccine was discussed.
Zhang
Hong-Ying
HY
Department of Biological Science and Technology and the State Key Laboratory of Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, Nanjing 210093, China. xiao99now@yahoo.com.cn
Sun
Shu-Han
SH
Guo
Ying-Jun
YJ
Chen
Zu-Huan
ZH
Huang
Li
L
Gao
Yuan-Jian
YJ
Wan
Bin
B
Zhu
Wei-Jia
WJ
Xu
Gen-Xing
GX
Wang
Jian-Jun
JJ
D000818:Animals; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics*; D007273:Injections, Intramuscular; D051379:Mice; D010957:Plasmids / Q000009:adverse effects / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution; D019444:Vaccines, DNA / Q000378:metabolism*; D014765:Viral Vaccines / Q000235:genetics / Q000378:metabolism*
D000939:Epitopes; D019444:Vaccines, DNA; D014765:Viral Vaccines