Affinity chromatography of porcine pepsin A using quinolin-8-ol as ligand.
Stationary phase containing quinolin-8-ol immobilized on macroporous methacrylate support for the affinity chromatography of porcine pepsin A is described. Optimized chromatographic conditions for separation of porcine pepsin A on this stationary phase were found investigating the influence of pH, concentration, ionic strength and chemical composition of the used mobile phases. The stationary phase shows a good reproducibility of chromatographic analyses (relative standard deviation, +/-2%), a high recovery (ca. 93%) and a satisfactory capacity (13 mg pepsin A/1 mL stationary phase) for porcine pepsin A. The obtained findings confirm the applicability of affinity chromatography on the stationary phase with immobilized quinolin-8-ol to the isolation and determination of porcine pepsin A.
Novotná
Lenka
L
Institute of Pathophysiology, First Faculty of Medicine, Charles University, U Nemocnice 5, 12853 Praha 2, Czech Republic. lenka.novotna@lf1.cuni.cz
Hrubý
Martin
M
Benes
Milan J
MJ
Kucerová
Zdenka
Z
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D006912:Hydroxyquinolines / Q000737:chemistry; D008024:Ligands; D010434:Pepsin A / Q000302:isolation & purification*; D013552:Swine
D006912:Hydroxyquinolines; D008024:Ligands; D010434:Pepsin A