[Dynamic pattern of gene expression in rat hearts responding to transient ischemia/reperfusion detected by cDNA microarray and cluster analysis].
OBJECTIVE
To analyze the pattern of gene expression programs during rat myocardial ischemia/reperfusion at multiple time points.


METHODS
Rat model of myocardial ischemia/reperfusion was established by repeating the occlusion and relaxation of left coronary artery. cDNA microarray was used to analyze the pattern of gene expression programs in rat myocardium at 1, 3, 6, 12, 24 hours after reperfusion. SOM cluster analysis was used to identify different cluster of genes in which each cluster had similar expression pattern.


RESULTS
Altogether 75, 779, 205, 155, and 166 genes were differentially expressed at 1, 3, 6, 12, 24 hours after the reperfusion respectively. Clusters analysis identified 12 clusters of genes in which each cluster had similar expression pattern.


CONCLUSIONS
Analysis of gene expression pattern revealed sequential induction of subsets of genes that characterize each response.
Yuan
Can
C
Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China. xianzhongxiao@hotmail.com
Zhao
Zhen-Yu
ZY
Liu
Ying
Y
Lu
Qing-Lan
QL
Wang
Qiu-Peng
QP
Xiao
Xian-Zhong
XZ
D000818:Animals; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000235:genetics*; D009206:Myocardium / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes; D051381:Rats; D013997:Time Factors
D015345:Oligonucleotide Probes