Self-made frits for nanoscale columns in proteomics.
We report here the production of self-made frits for nano-columns. The frits introduce a minor dead volume and can be placed in capillaries with a wide range of diameters (20-250 microm tested) in an extremely simple and low-cost procedure. The obtained columns appear to be comparable to "no-frit" columns with near-ideal chromatographic characteristics. We expect that this frit will be useful for the spotting of gradients onto MALDI plates but also where special ESI set-ups do not allow for "no-frit" solutions.
Maiolica
Alessio
A
The FIRC Institute of Molecular Oncology Foundation, Milan, Italy.
Borsotti
Dario
D
Rappsilber
Juri
J
D002853:Chromatography, Liquid / Q000295:instrumentation*; D002868:Chromosomal Proteins, Non-Histone; D013058:Mass Spectrometry / Q000295:instrumentation; D036103:Nanotechnology / Q000295:instrumentation*; D040901:Proteomics / Q000295:instrumentation; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014157:Transcription Factors
D002868:Chromosomal Proteins, Non-Histone; D014157:Transcription Factors