Induction of interleukin-6 and interleukin-8 gene expression by root canal sealers in human osteoblastic cells.
Histological investigations have demonstrated that root canal sealers can induce mild to severe inflammatory alternations. However, there is little information on the precise mechanisms about root canal sealers-induced inflammatory reaction. Dysregulated cytokine productions at local disease sites have been considered to be major contributors to the development of inflammatory diseases. Interleukin (IL)-6 and IL-8 released have been reported to play an important role in the pathogenesis of inflammation. The aim of this study was to investigate the effects of root canal sealers N2 (zinc-oxide eugenol based) and AH Plus (epoxy resin based) on the expression of IL-6 and IL-8 mRNA gene in human osteoblastic cell line U2OS cells. The levels of mRNAs were measured by the semi-quantitative reverse-transcriptase polymerase chain reaction analysis. The exposure of quiescent U2OS cells to N2 and AH Plus resulted in the induction of IL-6 and IL-8 mRNA gene expression (p < 0.05). The intensity of IL-8 mRNA gene was found to be significant higher than IL-6 mRNA gene (p < 0.05). Taken together, the activation of IL-6 and IL-8 mRNA gene expression may be one of the pathogenesis of zinc oxide-eugenol based and epoxy resin based root canal sealers-induced periapical inflammation.
Huang
Fu-Mei
FM
Department of Dentistry, College of Oral Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan.
Tsai
Chung-Hung
CH
Yang
Shun-Fa
SF
Chang
Yu-Chao
YC
D002478:Cells, Cultured; D004338:Drug Combinations; D004853:Epoxy Resins / Q000494:pharmacology*; D005054:Eugenol / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology*; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D015034:Zinc Oxide / Q000494:pharmacology*
D004338:Drug Combinations; D004853:Epoxy Resins; D015850:Interleukin-6; D016209:Interleukin-8; D012333:RNA, Messenger; D012387:Root Canal Filling Materials; C534916:epoxy resin-based root canal sealer; D005557:Formaldehyde; D005054:Eugenol; C003799:N2 Dental Cement; D015034:Zinc Oxide