[Metabolism of 125I labeled anti-p185 antibodies in vitro and their biodistribution in vivo].
OBJECTIVE
To study the cellular metabolism(RIA) in vitro and the biodistribution in vivo of (125)I labeled anti-p185 antibodies(murine mAb A21, mouse-human chimeric antibody A21scFv-Fc and single chain antibody A21 scFv), to provide the basis for clinical application in the diagnosis and treatment of tumor overexpressing p185.


METHODS
The specificity of binding of these antibodies to SKOV(3) known to highly express surface antigen p185 was assessd by FACS. The metabolism of the antibodies in SKOV(3) was assayed by cellular RIA. The biodistribution of radiolabeled antibodies was evaluated in nude mice bearing SKOV(3) tumor.


RESULTS
Cellular RIA demonstrated these antibodies were internalized after binding to cells, followed by degradation and deiodination in cells, (125)I was then exocytosed. In vivo test showed that these antibodies were concentrated in tumor, but no concentration of control antibody in tumor was found.


CONCLUSIONS
mAb A21, A21 scFv-Fc and A21 scFv can target human ovarian carcinoma cells (SKOV(3)) overexpressing p185 in vitro and in vivo and may be used in the diagnosis and treatment of tumors overexpressing p185.
Wang
Hai-yang
HY
Lab. of Cellular and Molecular Immunology, School of Life Science, University of Science and Technology of China, Hefei 230027, China. why7878@yahoo.com.cn
Liu
Hai-bo
HB
Dong
Yan-qiu
YQ
Yang
Jian-hong
JH
Cheng
Lian-sheng
LS
Liu
Jing
J
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000737:chemistry* / Q000378:metabolism* / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007205:Indium Radioisotopes / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000188:drug therapy; D011863:Radioimmunoassay; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology*
D000906:Antibodies; D007205:Indium Radioisotopes; D018719:Erb-b2 Receptor Tyrosine Kinases