title: Induction of apoptotic cell death by 2'-hydroxycinnamaldehyde is involved with ERK-dependent inactivation of NF-kappaB in TNF-alpha-treated SW620 colon cancer cells.
abstract: 2'-Hydroxycinnamaldehyde (HCA) inhibits cell growth of several human cancer cells with unknown mechanisms. We investigated the inhibitory effect of HCA on TNF-alpha-induced cell growth and possible signal pathway in SW620 colon cancer cells. HCA inhibited TNF-alpha-induced SW620 colon cell growth in time- and dose-dependent manner through induction of apoptotic cell death. Parallel with inhibitory effect on cell growth, HCA dose dependency inhibited TNF-alpha-induced activation of NF-kappaB accompanied with inhibition of the translocation of p50. HCA also induced expression of caspase-3 and Bax, but decreased Bcl-2. HCA furthermore activated ERK pathway, and ERK inhibitor reversed inhibitory effect of HCA on cell growth and transcriptional activation of NF-kappaB. These results demonstrate that HCA inhibits cell growth through induction of apoptotic cell death by ERK pathway-dependent NF-kappaB inactivation.
authors.affiliation: College of Pharmacy, Chungbuk National University, Heungduk-gu, Cheongju, South Korea.
authors.forename: Seung Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Chung Woo
authors.initials: CW
authors.lastname: Lee
authors.forename: Jae Woong
authors.initials: JW
authors.lastname: Lee
authors.forename: Myoung Suk
authors.initials: MS
authors.lastname: Choi
authors.forename: Dong Ju
authors.initials: DJ
authors.lastname: Son
authors.forename: Youn Bok
authors.initials: YB
authors.lastname: Chung
authors.forename: Chong Kil
authors.initials: CK
authors.lastname: Lee
authors.forename: Ki Wan
authors.initials: KW
authors.lastname: Oh
authors.forename: Dong Chul
authors.initials: DC
authors.lastname: Moon
authors.forename: Byoung Mog
authors.initials: BM
authors.lastname: Kwon
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002934:Cinnamates / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology*; D024202:Electrophoretic Mobility Shift Assay; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D002934:Cinnamates; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C117045:p-hydroxycinnamaldehyde; D048049:Extracellular Signal-Regulated MAP Kinases