Evaluations of blood compatibility via protein adsorption treatment of the vascular scaffold surfaces fabricated with polylactide and surface-modified expanded polytetrafluoroethylene for tissue engineering applications.
Blood compatibility was evaluated by short-term in vitro blood perfusion on candidate vascular scaffold surfaces of a biodegradable, porous polylactide scaffold and a chemically surface-modified expanded polytetrafluoroethylene (ePTFE) over a clinical ePTFE, by measuring blood cell adhesion either directly or after adsorption treatment with albumin and fibrinogen. The results indicated that the extent of blood cell adhesion was affected by scaffold surface properties and pre-adsorption of proteins such as fibrinogen and albumin. Surface morphologies and porosity of the scaffolds were characterized by scanning electron microscopy and porosimetry, and the amount of fibrinogen and albumin adsorbed on the scaffolds was measured and verified by employing radiolabeled C(14) albumin and I(125) fibrinogen by a scintillation counter and a gamma counter, respectively. Even though treatment of fibrinogen adsorption on the samples in advance led to higher induction of blood cell adhesion than those with no fibrinogen adsorption, the polylactide scaffold surface itself induced highest amount of the adhered blood cells in this study judged by analyses of their surface area. These results would be employed as guidance in determining a choice of the implant methods, in vitro versus in vivo tissue engineering, of the novel chemically modified ePTFE and the biodegradable polylactide scaffolds.
Choi
Yoon Jeong
YJ
Chemical Engineering Department, Seoul National University of Technology, Gongnung 2-dong 172, Nowon-Gu, Seoul 139-743, Korea.
Choung
Sung Kwang
SK
Hong
Choong Man
CM
Shin
In Soo
IS
Park
Sue Nie
SN
Hong
Seung Hwa
SH
Park
Han Ki
HK
Park
Young Hwan
YH
Son
Youngsook
Y
Noh
Insup
I
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001769:Blood; D004285:Dogs; D010949:Plasma; D011091:Polyesters / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry; D011506:Proteins; D013057:Spectrum Analysis; D023822:Tissue Engineering
D001672:Biocompatible Materials; D011091:Polyesters; D011506:Proteins; C033616:poly(lactide); D011138:Polytetrafluoroethylene