Blood-brain barrier-specific properties of a human adult brain endothelial cell line.
Establishment of a human model of the blood-brain barrier has proven to be a difficult goal. To accomplish this, normal human brain endothelial cells were transduced by lentiviral vectors incorporating human telomerase or SV40 T antigen. Among the many stable immortalized clones obtained by sequential limiting dilution cloning of the transduced cells, one was selected for expression of normal endothelial markers, including CD31, VE cadherin, and von Willebrand factor. This cell line, termed hCMEC/D3, showed a stable normal karyotype, maintained contact-inhibited monolayers in tissue culture, exhibited robust proliferation in response to endothelial growth factors, and formed capillary tubes in matrix but no colonies in soft agar. hCMEC/D3 cells expressed telomerase and grew indefinitely without phenotypic dedifferentiation. These cells expressed chemokine receptors, up-regulated adhesion molecules in response to inflammatory cytokines, and demonstrated blood-brain barrier characteristics, including tight junctional proteins and the capacity to actively exclude drugs. hCMEC/D3 are excellent candidates for studies of blood-brain barrier function, the responses of brain endothelium to inflammatory and infectious stimuli, and the interaction of brain endothelium with lymphocytes or tumor cells. Thus, hCMEC/D3 represents the first stable, fully characterized, well-differentiated human brain endothelial cell line and should serve as a widely usable research tool.
Weksler
B B
BB
Institut Cochin, CNRS UMR 8104-INSERM U567, Université René Descartes, Paris, France. babette@med.cornell.edu
Subileau
E A
EA
Perrière
N
N
Charneau
P
P
Holloway
K
K
Leveque
M
M
Tricoire-Leignel
H
H
Nicotra
A
A
Bourdoulous
S
S
Turowski
P
P
Male
D K
DK
Roux
F
F
Greenwood
J
J
Romero
I A
IA
Couraud
P O
PO
D000362:Agar / Q000737:chemistry; D000818:Animals; D015703:Antigens, CD; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis / Q000235:genetics; D001812:Blood-Brain Barrier / Q000187:drug effects; D015153:Blotting, Western; D001921:Brain / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015820:Cadherins / Q000096:biosynthesis; D002196:Capillaries / Q000473:pathology; D002417:Cattle; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003094:Collagen / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D004338:Drug Combinations; D018432:Drug Resistance, Multiple; D042783:Endothelial Cells / Q000166:cytology* / Q000473:pathology; D004730:Endothelium, Vascular / Q000166:cytology / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D007797:Laminin / Q000494:pharmacology; D016086:Lentivirus / Q000235:genetics; D008214:Lymphocytes / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D010477:Perfusion; D010539:Permeability; D010641:Phenotype; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D011509:Proteoglycans / Q000494:pharmacology; D012313:RNA / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation; D014841:von Willebrand Factor / Q000096:biosynthesis; D000099152:Cadherin 5
D000362:Agar; D015703:Antigens, CD; D000952:Antigens, Polyomavirus Transforming; D015820:Cadherins; D003094:Collagen; D016207:Cytokines; D004338:Drug Combinations; D007797:Laminin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011509:Proteoglycans; D012313:RNA; D019098:Telomerase; D014841:von Willebrand Factor; D000099152:Cadherin 5; C059485:matrigel