title: Galatosylation and sialylation of mammalian glycoproteins produced by baculovirus-madiated gene expression in insect cells.
abstract: The baculovirus expression vector system (BEVS) is used extensively for the production of proteins from exogenous cDNAs. However, BEVS is not ideal for pharmaceutical production of glycoproteins owing to the properties of the N-glycans in the expressed products and that insect cells lack several of the enzymes required for mammalian-type N-glycan synthesis. This study describes the effective mammalian-like production of glycoproteins, such as beta-1,4-galactosyltransferase and alpha-2,6-sialyltransferase, in the insect cell line Sf9.
authors.affiliation: Department of Agricultural Biology, National Institute of Agricultural Science and Technology, 441-100, RDA, Suwon, Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Yun
authors.forename: Tae-Won
authors.initials: TW
authors.lastname: Goo
authors.forename: Sung-Wan
authors.initials: SW
authors.lastname: Kim
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Choi
authors.forename: Jae-Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Seok-Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: O-Yu
authors.initials: OY
authors.lastname: Kwon
mesh_terms: D000818:Animals; D016367:Baculoviridae; D006031:Glycosylation; D006801:Humans; D046188:Protein Modification, Translational / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012799:Sialyltransferases / Q000096:biosynthesis* / Q000235:genetics; D018411:Spodoptera; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D011994:Recombinant Proteins; D012799:Sialyltransferases; C029156:N-acetyllactosaminide alpha-2,3-sialyltransferase; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase