[Influences of FVP1 on the curative and negative effects of CTX].
OBJECTIVE
To observe the influences of FVP1 on both curative and negative effects of CTX.


METHODS
The present study included two parts of experiments. In the part 1, 0.2 mL of 1 x 10(7) mL(-1) of S180 cells were inoculated in the subcutaneous layer of the right armpit of mice. All the mice were randomly divided into 3 groups: control group, in which mice were given with normal saline in 10 consecutive days, CTX group, in which mice were injected with 30 mg of CTX in the first and third days and saline in the other 8 days during the 10 consecutive days of treatment, and FVP1 and CTX group, in which the mice were injected with 30 mg x kg(-1) of CTX in the first and third days and FVP1 at 10 mg x kg(-1) in all 10 consecutive days of treatment. After above 10-day treatment , all the mice were killed and the tumor body was taken out and weighed to calculate the inhibiting rates on tumor. In the part 2 of experiments all the mice were divided into 3 groups: Normal control group, in which mice were not treated with any drugs, CTX-induced model group of inhibiting immune system, in which mice were injected with CTX at dose of 10 mg x kg(-1) in first two days and saline in the following 7 days; and small-, meddle-and large-dosage of FVP1 groups, in which mice were injected with CTX at the same dose as above in first two days and FVP1 intraperitoneally at 5, 10 and 20 mg x kg(-1) respectively in the following 7 days. CTX group was regarded as the control model. After the treatment, the peripheral white cells, thymus index, spleen index, the phagocytic power of macrophage of abdominal cavity, lymphocyte trastation rate and the activity of NK cell were detected.


RESULTS
(DFVP1 plus small dose of CTX obviously enhanced the inhibiting rate of CTX on tumor in the mice inoculated with S180 cells. (2) FVP1 at the different dose obviously antagozized CTX-induced leucopenia, atrophy, reduction of the phagocytic power of macrophage in abdominal cavity and restored the function of lymphocyte translation and the activity of NK cells.


CONCLUSIONS
FPV1 could enhance the curative effect of CTX in depressing tumor and attenuate the negative effect of CTX in inhibiting the function of immune system.
Yuan
Qiang
Q
Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China.
Chen
Zhi-Yun
ZY
Yan
Mao-Xiang
MX
D000363:Agaricales / Q000737:chemistry; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000009:adverse effects / Q000494:pharmacology; D045744:Cell Line, Tumor; D003520:Cyclophosphamide / Q000009:adverse effects / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D007694:Killer Cells, Natural / Q000187:drug effects; D007970:Leukopenia / Q000139:chemically induced; D008213:Lymphocyte Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000502:physiology; D051379:Mice; D009368:Neoplasm Transplantation; D010587:Phagocytosis / Q000187:drug effects*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011897:Random Allocation; D012510:Sarcoma 180 / Q000473:pathology*
D018906:Antineoplastic Agents, Alkylating; D011134:Polysaccharides; D003520:Cyclophosphamide