T- and B-cell clonality and frequency of human herpes viruses-6, -8 and Epstein Barr virus in angioimmunoblastic T-cell lymphoma.
Angioimmunoblastic T-cell lymphoma (T-AIL) is a peripheral T-cell lymphoma of unknown etiology. Previous clonality studies have shown a heterogeneous composition of this disease with varying restrictions of B- and T-cell populations in the tumour. For the first time in a single study and in the same pathological materials, we have analysed, lymphoid cell clonality and occurrence of human herpes viruses and Epstein Barr virus. Of 18 cases 12 (66.6%) had clonal T- and three (16.6%) had clonal B-cells. Presence of the lymphotropic viral genome of HHV6 was detected in four of 18 lymph node biopsies from T-AIL patients (22%), all were TCRgamma clonal. No HHV8 were found. Epstein Barr genome was found in 40% of cases. There was no significant association between T-cell clonality and HHV-6 or EBV infection, or between B-cell clonality and any virus infection. We conclude that T-AIL is a biologically and clinically heterogeneous entity whose true nature remains to be clarified.
Vrsalovic
Maruska Marusic
MM
Institute of Clinical Chemistry, Division of Molecular Diagnostics, Merkur University Hospital, Zagreb, Croatia.
Korac
Petra
P
Dominis
Mara
M
Ostojic
Slobodanka
S
Mannhalter
Christine
C
Kusec
Rajko
R
D016175:B-Lymphocyte Subsets / Q000473:pathology*; D002999:Clone Cells / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics; D020031:Epstein-Barr Virus Infections / Q000473:pathology / Q000821:virology; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D005803:Genes, Immunoglobulin; D019675:Genes, T-Cell Receptor gamma; D006566:Herpesviridae Infections / Q000473:pathology* / Q000821:virology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D015654:Herpesvirus 6, Human / Q000302:isolation & purification*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000473:pathology* / Q000821:virology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D016411:Lymphoma, T-Cell, Peripheral / Q000473:pathology* / Q000821:virology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics; D019349:Roseolovirus Infections; D016176:T-Lymphocyte Subsets / Q000473:pathology*; D014412:Tumor Virus Infections / Q000473:pathology* / Q000821:virology
D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains; D016692:Receptors, Antigen, T-Cell, gamma-delta