title: Oxazolidinones inhibit cellular proliferation via inhibition of mitochondrial protein synthesis.
abstract: The oxazolidinones are a relatively new structural class of antibacterial agents that act by inhibiting bacterial protein synthesis. The oxazolidinones inhibit mitochondrial protein synthesis, as shown by [35S]methionine incorporation into intact rat heart mitochondria. Treatment of K562 human erythroleukemia cells with the oxazolidinone eperezolid resulted in a time- and concentration-dependent inhibition of cell proliferation. The cells remained viable, but an increase in doubling time was observed with eperezolid treatment. Inhibition was reversible, since washing and refeeding of cells in the absence of compound resulted in a resumption of growth. The growth-inhibitory effect of the oxazolidinones did not appear to be cell type specific, and inhibition of CHO and HEK cells also was demonstrated. Treatment of cells resulted in a decrease in mitochondrial cytochrome oxidase subunit I levels, consistent with an inhibition of mitochondrial protein synthesis. Eperezolid caused no growth inhibition of rho zero (rho0) cells, which contain no mitochondrial DNA; however, the growth of the parent 143B cells was inhibited. These results provide a direct demonstration that the inhibitory effect of eperezolid in mammalian cells is the result of mitochondrial protein synthesis inhibition.
authors.affiliation: Department of Antibacterial Pharmacology, Pfizer, Ann Arbor, MI 48105, USA.
authors.forename: Eva E
authors.initials: EE
authors.lastname: Nagiec
authors.forename: Luping
authors.initials: L
authors.lastname: Wu
authors.forename: Steve M
authors.initials: SM
authors.lastname: Swaney
authors.forename: John G
authors.initials: JG
authors.lastname: Chosay
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Ross
authors.forename: Joan K
authors.initials: JK
authors.lastname: Brieland
authors.forename: Karen L
authors.initials: KL
authors.lastname: Leach
mesh_terms: D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D020014:K562 Cells; D000069349:Linezolid; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism*; D010080:Oxazoles / Q000494:pharmacology*; D023303:Oxazolidinones / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D000081:Acetamides; D010080:Oxazoles; D023303:Oxazolidinones; D011500:Protein Synthesis Inhibitors; C098011:eperezolid; D000069349:Linezolid