title: Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil.
abstract: We report the frequency of the different diarrheagenic Escherichia coli (DEC) categories isolated from children with acute endemic diarrhea in Salvador, Bahia. The E. coli isolates were investigated by colony blot hybridization with the following genes probes: eae, EAF, bfpA, Stx1, Stx2, ST-Ih, ST-Ip, LT-I, LT-II, INV, and EAEC, as virulence markers to distinguish typical and atypical EPEC, EHEC/STEC, ETEC, EIEC, and EAEC. Seven of the eight categories of DEC were detected. The most frequently isolated was atypical EPEC (10.1%) followed by ETEC (7.5%), and EAEC (4.2%). EHEC, STEC, EIEC, and typical EPEC were each detected once. The strains of ETEC, EAEC, and atypical EPEC belonged to a wide variety of serotypes. The serotypes of the others categories were O26:H11 (EHEC), O21:H21 (STEC), O142:H34 (typical EPEC), and O:H55 (EIEC). We also present the clinical manifestations and other pathogenic species observed in children with DEC. This is the first report of EHEC and STEC in Salvador, and one of the first in Brazil.
authors.affiliation: Laboratório Especial de Microbiologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil. mrfranzolin@butantan.gov.br
authors.forename: Marcia Regina
authors.initials: MR
authors.lastname: Franzolin
authors.forename: Rosely Cabette Barbosa
authors.initials: RC
authors.lastname: Alves
authors.forename: Rogéria
authors.initials: R
authors.lastname: Keller
authors.forename: Tânia Aparecida Tardelli
authors.initials: TA
authors.lastname: Gomes
authors.forename: Lothar
authors.initials: L
authors.lastname: Beutin
authors.forename: Mauricio Lima
authors.initials: ML
authors.lastname: Barreto
authors.forename: Craig
authors.initials: C
authors.lastname: Milroy
authors.forename: Agostino
authors.initials: A
authors.lastname: Strina
authors.forename: Hugo
authors.initials: H
authors.lastname: Ribeiro
authors.forename: Luiz Rachid
authors.initials: LR
authors.lastname: Trabulsi
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D001938:Brazil / Q000453:epidemiology; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D019353:Endemic Diseases; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006801:Humans; D010641:Phenotype; D015995:Prevalence