Transcriptional signaling from membrane raft-associated glucocorticoid receptor.
The contribution of plasma membrane-associated glucocorticoid receptor (GR) to transcriptional signaling is unclear. We observed GR in low-density detergent-resistant membrane (DRM) rafts derived from human hepatoma Hep3B cells in complexes with caveolin-1, HSP90, and STAT3. In transient transfection assays, GR-stimulated transcriptional signaling was reversibly inhibited by membrane-raft disrupters filipin III and progesterone. These data provide clear evidence for a functional contribution of DRM-associated GR to transcriptional signaling.
Jain
Sudhir
S
Department of Pathology, New York Medical College, Valhalla, NY 10595, USA.
Li
Yanna
Y
Kumar
Ashok
A
Sehgal
Pravin B
PB
D001483:Base Sequence; D051242:Caveolin 1; D022461:Caveolins / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D005372:Filipin / Q000494:pharmacology; D006801:Humans; D017830:Octoxynol / Q000737:chemistry; D009841:Oligonucleotides; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction; D014158:Transcription, Genetic
C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; D009841:Oligonucleotides; D011965:Receptors, Glucocorticoid; D005372:Filipin; D017830:Octoxynol