Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo.
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank.
Nascimento
Elvira Maria Mendes
EM
Laboratório de Rickettsias, Instituto Adolfo Lutz, São Paulo, SP, Brazil.
Gehrke
Flávia de Sousa
Fde S
Maldonado
Rosa Amélia
RA
Colombo
Silvia
S
Silva
Luiz Jacintho da
LJ
Schumaker
Teresinha Tizu Sato
TT
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D001938:Brazil; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D004269:DNA, Bacterial / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D019353:Endemic Diseases; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012282:Rickettsia Infections / Q000175:diagnosis* / Q000821:virology; D012284:Rickettsia rickettsii / Q000235:genetics*; D013987:Ticks / Q000382:microbiology; D014709:Vero Cells
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial