[Effects of bone marrow fibroblasts conditioned media on the expansion of megakaryocytes in vitro].
OBJECTIVE
To investigate the effect of bone marrow fibroblasts conditioned media on the expansion of megakaryocytes in vitro.


METHODS
Serum-free bone marrow fibroblasts conditioned medium (BMF-CM) was prepared and collected. The effect of BMF-CM or BMF-CM combined with IL-11 on the expansion of mature megakaryocytes and the effect of BMF-CM on the expansion of megakaryocytic progenitors were observed. RT-PCR was applied to determine the expression of NF-E2 mRNA in human megakaryoblastic leukemia cell line--Meg-0l.


RESULTS
BMF-CM promoted the expansion of mature megakaryocytes. The effect of BMF-CM combined with IL-11 on the expansion of mature megakaryocytes was further enhanced. BMF-CM promoted the expansion of megakaryocytic progenitors. NF-E2 mRNA expression in megakaryocytic cell lines Meg-0l was enhanced when cultures were exposed to 30% BMF-CM for 4 hours, compared with control cultures exposed to IMDM for 4 hours.


CONCLUSIONS
BMF-CM can promote the expansion of megakaryocytes in vitro.
Zhou
Xiao-ying
XY
Laboratory of Experimental Hematology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Huang
Yan-hong
YH
Wang
Qi-ru
QR
Lu
Guang-xiu
GX
Tan
Meng-qun
MQ
D001854:Bone Marrow Cells / Q000166:cytology*; D002455:Cell Division; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology*; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned