title: [Testosterone's effect on tPA and PAI-1 mRNA levels of HUVEC].
abstract: OBJECTIVE
  To investigate the effect of testosterone with varied concentrations on the tPA and PAI-1 mRNA levels of Human umbilical vein endothelial cells (HUVEC).
  
  
  METHODS
  HUVEC within 2 - 3 passages were cultured with testosterone (3, 30, 3 x 10(3), 3 x 10(4) nmol/L) , and the control confluent cells were cultured in the same medium without steroid for 48 hours. RT-PCR was carried out to detect tPA and PAI-1 mRNA levels.
  
  
  RESULTS
  tPA mRNA level increased, while PAI-1 mRNA levels decreased significantly, at the testosterone concentrations ranging from 3 to 3 x 10(3) nmol/L (P < 0.05). Both tPA and PAI-1 mRNA level decreased obviously of 3 x 10(4) nmol/L group.
  
  
  CONCLUSIONS
  The results indicated that testosterone could stimulate tPA gene expression, while decreased PAI-1 mRNA level of HUVEC, which suggested that testosterone might have beneficial effects on preventing male's thrombotic diseases.
authors.affiliation: Department of Cardiology, First Affiliated Hospital to Medical College of Shantou University, Shantou, Guang-dong 515041, China. cherry1970@sina.com
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Li
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Wang
authors.forename: Xue-Rui
authors.initials: XR
authors.lastname: Tan
mesh_terms: D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013739:Testosterone / Q000494:pharmacology*; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis* / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger; D013739:Testosterone; D010959:Tissue Plasminogen Activator