Auramine orange stain with fluorescence microscopy is a rapid and sensitive technique for the detection of cervical lymphadenitis due to mycobacterial infection using fine needle aspiration cytology: a case series.
OBJECTIVE
We sought to evaluate the effectiveness of the auramine orange (AO) stain in diagnosing mycobacterial cervical adenitis (MCA) from fine needle aspiration (FNA) cytology.


METHODS
A retrospective review of 19 patients evaluated at 2 urban hospitals from 2000 to 2003 for suspected MCA. FNA specimens were inoculated to culture media and had direct smears stained by the auramine acid fast method.


RESULTS
Mycobacteria were identified in 16 (84.2%) of 19 AO-stained FNA specimens, with results available within 4 hours. Corresponding cultures were positive for mycobacteria in 12 specimens, 9 tuberculous and 3 nontuberculous, and grew Mycobacterium tuberculosis from the 3 AO-negative specimens. Three of the 4 patients with negative cultures had previously taken anti-mycobacterial medications.


CONCLUSIONS
The AO stain with fluorescence microscopy is a sensitive and rapid method for detecting tuberculous and nontuberculous mycobacteria. It is a valuable tool for the otolaryngologists and pathologists in the diagnosis of MCA.
Cheng
Alan G
AG
Department of Otolaryngology--Head and Neck Surgery, University of Washington Medical Center, Seattle, 98195, USA. aglcheng@u.washington.edu
Chang
Anthony
A
Farwell
D Greg
DG
Agoff
S Nicholas
SN
D000328:Adult; D001576:Benzophenoneidum; D044963:Biopsy, Fine-Needle; D004396:Coloring Agents; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods; D008875:Middle Aged; D015269:Mycobacterium avium Complex / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014388:Tuberculosis, Lymph Node / Q000382:microbiology* / Q000473:pathology*
D004396:Coloring Agents; D001576:Benzophenoneidum