The vanB2 gene cluster of the majority of vancomycin-resistant Enterococcus faecium isolates from Taiwan is associated with the pbp5 gene and is carried by Tn5382 containing a novel insertion sequence.
Thirty-two vanB2 Enterococcus faecium isolates were found to harbor Tn5382. Twenty-four isolates had a 1,419-bp sequence inserted within the open reading frame (ORF) C of Tn5382. This 1,419-bp sequence contained a 638-bp ORF with a 72% amino acid sequence homology with the transposase gene of IS150. Thirty isolates had the pbp5 gene linked to Tn5382.
Lu
Jang-Jih
JJ
Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, Taipei, Taiwan 114, Republic of China. jjl@mail.ndmctsgh.edu.tw
Chang
Tein-Yao
TY
Perng
Cherng-Lih
CL
Lee
Shih-Yi
SY
D001426:Bacterial Proteins / Q000235:genetics*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D013624:Taiwan; D020713:Vancomycin Resistance / Q000235:genetics*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; C079913:VanB protein, Enterococcus