A novel chemiluminescence method for the determination of orciprenaline based on ferricyanide-rhodamine 6G.
A novel flow injection chemiluminescence method for the determination of orciprenaline was developed. The method is based on the chemiluminescence (CL) reaction of orciprenaline with potassium ferricyanide in sodium hydroxide medium, sensitized by the fluorescent dye rhodamine 6G. The proposed procedure allows quantitation of orciprenaline in the concentration range 0.01-1.2 microg/mL, with a detection limit of 7.2 x 10(-3) microg/mL. The relative standard deviation (RSD) is 2.7% for 0.1 microg/mL orciprenaline (n = 9). The sampling frequency was calculated at approximately 120/h. The method was successfully applied to the determination of orciprenaline in pharmaceutical preparations. A brief discussion on the possible CL reaction mechanism is presented.
Lv
Yi
Y
Department of Chemistry, Key Laboratory for Atomic and Molecular Nanosciences of the Education Ministry, Tsinghua University, Beijing 100084, People's Republic of China.
Zhao
Rui
R
Zhu
Zhenli
Z
Xu
Xianshun
X
Zhang
Xinrong
X
D005292:Ferricyanides / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D009921:Metaproterenol / Q000032:analysis* / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*
D005292:Ferricyanides; D012235:Rhodamines; C026188:rhodamine 6G; D009921:Metaproterenol