[Effects of estradoil valerate on osteoporosis in ovariectomized rats].
OBJECTIVE
Compared with 17beta-estradiol, to evaluate the effects of estradiol valerate on osteopenia in ovariectomized rats.


METHODS
Forty two six months old female Wistar rats were randomly divided into 5 groups performed ovariectomy (OVX) or sham-operation. (1) 6 weeks after OVX (Ovxb, n = 8): sacrificed at 6 weeks after OVX; (2) Sham ovariectomized (n = 8); (3) 14 weeks after OVX (Ovxe, n = 8); (4) 17beta-estradoil treated group (O + E, n = 9, with 17beta-estradiol 20 microg x kg(-1) x d(-1) Sc.); (5) Estradoil valerate treated group (O + EV, n = 9, with estradiol valerate 800 microg x kg(-1) x d(-1) po). Treatment started 6 weeks after OVX and lasted for 8 weeks. Histomorphometry analysis of tibia, peripheral quantitative computed tomography (pQCT) scanning of femur, bone biomechanical test in femur were performed.


RESULTS
OVX induced osteopenia and increase of bone turnover. Compared with Ovxe, O + E and O + EV caused an evident reduction of urinary deoxypyridinoline/creatinine, a bone resorption marker, by 54.6% and 77.4% respectively (all P < 0.01). Both O + E and O + EV lowered the high level of eroded surface, mineral surface and bone formation rate induced by OVX. An increase of 122.3% and 119.7% in trabecular bone volume respectively in O + E and O + EV group, compared with Ovxe group. Microarchitecture of trabeculea also improved in those two groups. The trabeculae bone mineral density determined by pQCT were significantly increased in O + E and O + EV compared with Ovxe group (99.5% and 128.4% P < 0.01). Similarly, significant increases were found in cancellouse maximal load and canceallous stiffness of distal femur in O + E, but not O + EV. There was no significant difference in these changes between the group O + E and O + EV.


CONCLUSIONS
Oral estradiol valerate performed effects similar to those of 17beta-estradiol Sc. in inhibiting bone turnover, protecting bone loss and increasing bone mineral density of trabecular bone.
Xia
W
W
Department of Endocrinology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, China.
Meng
X
X
Xing
X
X
D000818:Animals; D015519:Bone Density / Q000187:drug effects; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D016723:Bone Remodeling / Q000187:drug effects*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D005260:Female; D005269:Femur; D010024:Osteoporosis / Q000517:prevention & control*; D010052:Ovariectomy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia
D050071:Bone Density Conservation Agents; D004958:Estradiol