Ciprofloxacin-induced DNA damage in primary culture of rat astrocytes and protection by Vitamin E.
Fluoroquinolones are generally well-tolerated antibiotics in patients. Gastrointestinal, central nervous system, and dermatological adverse events were the most frequent unwanted effects during therapy with these drugs. However, the mechanism of these adverse effects has not yet been elucidated. The aim of this study was to investigate the possible DNA damage-inducing effect of a fluoroquinolone (FQ) antibiotic, ciprofloxacin (CPFX) on primary culture of rat astrocytes. For this purpose, the cultured cells were incubated with various concentrations of CPFX, and DNA damage was monitored by comet assay. Our results showed a concentration-dependent induction of DNA damage by CPFX. Pretreatment of cells with Vitamin E for 4h provided partial protection against this effect. The data obtained in this study suggest that CPFX-induced DNA damage might be related to oxidative stress and should be considered for further mechanistic studies of central nervous system toxicity of CPFX.
Gürbay
Aylin
A
Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, 06100 Ankara, Turkey. Aylingurbay@hotmail.com
Gonthier
Brigitte
B
Signorini-Allibe
Nathalie
N
Barret
Luc
L
Favier
Alain
A
Hincal
Filiz
F
D000818:Animals; D000831:Animals, Newborn; D000890:Anti-Infective Agents; D000975:Antioxidants / Q000494:pharmacology; D001253:Astrocytes / Q000378:metabolism*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002939:Ciprofloxacin; D020552:Comet Assay; D019610:Cytoprotection / Q000187:drug effects*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000494:pharmacology*
D000890:Anti-Infective Agents; D000975:Antioxidants; D014810:Vitamin E; D002939:Ciprofloxacin