title: Involvement of SIP1 in positioning of somite boundaries in the mouse embryo.
abstract: Periodical production of somites provides an excellent model system for understanding genesis of metameric structures underlying embryonic development. This study reports production of somites with roughly half rostro-caudal length in homozygous Sip1 (Smad-interacting protein 1) knockout mouse embryos. This altered periodicity of somitogenesis is caused by the rostral expansion of the expression domain of genes involved in the maintenance of unsegmented state of paraxial mesoderm, e.g., Fgf8, Wnt3a, Dll3, and Tbx6. This is accompanied by the rostral extension of oscillatory gene expression such as L-fng, Hes7, and Dll1, and the rostrally shifted termination of Raldh2 expression that continues from the anterior embryonic side. The phenotype of Sip1-/- embryo introduces a new molecular component SIP1 in positioning of somite boundaries, and provides support for the current "clock and wavefront" model.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.
authors.forename: Mitsuji
authors.initials: M
authors.lastname: Maruhashi
authors.forename: Tom
authors.initials: T
authors.lastname: Van De Putte
authors.forename: Danny
authors.initials: D
authors.lastname: Huylebroeck
authors.forename: Hisato
authors.initials: H
authors.lastname: Kondoh
authors.forename: Yujiro
authors.initials: Y
authors.lastname: Higashi
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism*; D051524:Fibroblast Growth Factor 8 / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006579:Heterozygote; D006720:Homozygote; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D007438:Introns; D008565:Membrane Proteins / Q000378:metabolism; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D009991:Oscillometry; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D019170:Somites / Q000378:metabolism; D020825:T-Box Domain Proteins; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism; D060508:Wnt3 Protein; D060509:Wnt3A Protein
chemical_list: D000445:Aldehyde Oxidoreductases; D051792:Basic Helix-Loop-Helix Proteins; D051524:Fibroblast Growth Factor 8; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D051153:Wnt Proteins; D060508:Wnt3 Protein; D060509:Wnt3A Protein; C496666:DLL3 protein, human; C496457:Fgf8 protein, mouse; C430525:Hes7 protein, mouse; C491561:Sip1 protein, mouse; C102932:Tbx6 protein, mouse; C555510:WNT3A protein, human; C555511:Wnt3a protein, mouse; C470579:RALDH2 protein, mouse