[A template DNA preparation method for PCR and RAPD reaction by using alkali-treated wheat leaf tissues].
In this paper we introduce a new method of PCR and RAPD reaction by using a small quantity of wheat leaf tissue with alkali treatment. It can be used either to detect target gene or to analyze polymorphism of wheat varieties. This method is simple and reliable, especially for a large-scale detection.
Wang
Xiu-Feng
XF
Key Laboratory of Rice Genetics and Breeding for Agricultural Ministry of China, AAAS, Hefei 230031, China. bioinfo@mail.hf.ah.cn
Yang
Jian-Bo
JB
Wu
Li-Fang
LF
Li
Li
L
Xiang
Tai-He
TH