title: Effect of daidzin, genistin, and glycitin on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts.
abstract: OBJECTIVE
  To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic transdifferentiation of osteoblasts.
  
  
  METHODS
  MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed.
  
  
  RESULTS
  Daidzin, genistin, and glycitin 1*10(-8), 5*10(-7), 1*10(-6), 5*10(-6), and 1*10(-5) mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5*10(-7) mol/L and genistin 1*10(-6) mol/L promoted the osteogenesis of MSC. Genistin 1*10(-8), 5*10(-7), 1*10(-6), 5*10(-6), and 1*10(-5) mol/L and glycitin 1*10(-8), 1*10(-6), and 1*10(-5) mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1*10(-8), 5*10(-7), 1*10(-6), 5*10(-6), and 1*10(-5) mol/L all inhibited the adipocytic transdifferentiation of osteoblasts.
  
  
  CONCLUSIONS
  Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferen-tiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis.
authors.affiliation: State Key Laboratory of Biomembrane, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
authors.forename: Xiang-hui
authors.initials: XH
authors.lastname: Li
authors.forename: Jin-chao
authors.initials: JC
authors.lastname: Zhang
authors.forename: Sen-fang
authors.initials: SF
authors.lastname: Sui
authors.forename: Meng-su
authors.initials: MS
authors.lastname: Yang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D007529:Isoflavones / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects*; D048789:Phytoestrogens / Q000494:pharmacology*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects
chemical_list: D007529:Isoflavones; D048789:Phytoestrogens; C040641:genistin; C013908:daidzin; C433283:glycitin