title: Cloning of a human parvovirus by molecular screening of respiratory tract samples.
abstract: The identification of new virus species is a key issue for the study of infectious disease but is technically very difficult. We developed a system for large-scale molecular virus screening of clinical samples based on host DNA depletion, random PCR amplification, large-scale sequencing, and bioinformatics. The technology was applied to pooled human respiratory tract samples. The first experiments detected seven human virus species without the use of any specific reagent. Among the detected viruses were one coronavirus and one parvovirus, both of which were at that time uncharacterized. The parvovirus, provisionally named human bocavirus, was in a retrospective clinical study detected in 17 additional patients and associated with lower respiratory tract infections in children. The molecular virus screening procedure provides a general culture-independent solution to the problem of detecting unknown virus species in single or pooled samples. We suggest that a systematic exploration of the viruses that infect humans, "the human virome," can be initiated.
authors.affiliation: Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden. tobias.allander@karolinska.se
authors.forename: Tobias
authors.initials: T
authors.lastname: Allander
authors.forename: Martti T
authors.initials: MT
authors.lastname: Tammi
authors.forename: Margareta
authors.initials: M
authors.lastname: Eriksson
authors.forename: Annelie
authors.initials: A
authors.lastname: Bjerkner
authors.forename: Annika
authors.initials: A
authors.lastname: Tiveljung-Lindell
authors.forename: Björn
authors.initials: B
authors.lastname: Andersson
mesh_terms: D002675:Child, Preschool; D003001:Cloning, Molecular; D005260:Female; D016679:Genome, Viral; D006801:Humans; D015994:Incidence; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology; D017992:Parvovirus / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D012140:Respiratory Tract Diseases / Q000821:virology*