Improvement of the lectin-antibody enzyme immunoassay of the alphafetoprotein carbohydrate chain for automation with the enzyme immunoassay robot.
The lectin-antibody enzyme immunoassay of the alphafetoprotein-L3 carbohydrate chain, a tumor marker of liver cancer, has not been automated. We improved the technique of the assay for automation. Consequently, alphafetoprotein-L3 and total alphafetoprotein were detected with two lectins using an automatic paramagnetic bead handling robot. This indicates that the improved method is potentially applicable to the automated enzyme immunoassay robot.
Tamano
Koichi
K
National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
Sugiura
Mika
M
Natsuki
Jun
J
Sawakami-Kobayashi
Kazumi
K
Tajima
Hideji
H
Machida
Masayuki
M
D002241:Carbohydrates / Q000032:analysis*; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D012371:Robotics; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
D002241:Carbohydrates; D000509:alpha-Fetoproteins