[The study of coupling agents for the phosphorylation of 3'-azido-3'-deoxythymidine with (P32)orthophosphoric acid].
The kinetics of 3'-azido-3'-deoxythymidine phosphorylation with [32P]orthophosphoric acid was studied in the presence of various coupling agents. The most effective method with the use of BrCN provided the isolation of the target 3'-azido-3'-deoxythymidine 5'-[32P]monophosphate in 46% yield and a high specific radioactivity (>100 Ci/mmol).
Ianvarev
D V
DV
Shirokova
E A
EA
Skoblov
Iu S
IuS
D000097:Acetonitriles / Q000737:chemistry; D054306:Dideoxynucleotides; D010756:Phosphoric Acids / Q000737:chemistry*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D013942:Thymine Nucleotides / Q000737:chemistry*; D015215:Zidovudine / Q000031:analogs & derivatives* / Q000737:chemistry
D000097:Acetonitriles; D054306:Dideoxynucleotides; D010756:Phosphoric Acids; D010761:Phosphorus Radioisotopes; D013942:Thymine Nucleotides; C057287:3'-azido-3'-deoxythymidine 5'phosphate; D015215:Zidovudine; C030242:phosphoric acid