title: [The role of Salmonella and Yersinia in the pathogenesis of spondyloarthropathies and rheumatoid arthritis. I. Detection of bacteria, bacterial DNA, lipopolysaccharide and ECA antigen in synovial fluid or blood].
abstract: To investigate the role of Salmonella and Yersinia in the pathogenesis of spondyloarthropathies and rheumatoid arthritis synovial specimens from 92 patients were analysed for the presence of bacterial DNA with the use of polymerase chain reaction and for the presence of lipopolysaccharide and enterobacterial common antigen (ECA) with the use of Dot-ELISA. In addition, peripheral blood samples were available for PCR analysis from 68 patients. Salmonella and Yersinia chromosomal DNA was not found in any of the synovial specimens and blood samples from the patients. All of the synovial fluids were also culture-negative. Salmonella LPS antigens were observed in 8 (8.6%), Yersinia in 20 (21.7%) and ECA antigens in 32 (34.9%) synovial specimens. Our findings revealed the presence of bacterial degradation products, but not bacteria from the genus Salmonella and Yersinia or their DNA in the synovial fluid or blood of patients with spondyloarthropathies and rheumatoid arthritis.
authors.affiliation: Zakład Bakteriologii PZH w Warszawie.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Rastawicki
authors.forename: Marek
authors.initials: M
authors.lastname: Jagielski
authors.forename: Rafał
authors.initials: R
authors.lastname: Gierczyński
authors.forename: Halina
authors.initials: H
authors.lastname: Garwolińska
authors.forename: Brygida
authors.initials: B
authors.lastname: Kwiatkowska
authors.forename: Anna
authors.initials: A
authors.lastname: Filipowicz-Sosnowska
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis; D001172:Arthritis, Rheumatoid / Q000382:microbiology*; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis; D008297:Male; D012475:Salmonella / Q000302:isolation & purification*; D025242:Spondylarthropathies / Q000382:microbiology*; D013582:Synovial Fluid / Q000382:microbiology*; D015007:Yersinia / Q000302:isolation & purification*
chemical_list: D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D008070:Lipopolysaccharides; C017286:enterobacterial common antigen