title: Detection of enteroviruses in renal biopsies from patients with immunoglobulin A nephropathy.
abstract: Viruses have been suspected to be one of the causes of IgA nephropathy (IgAN). Recent studies have detected viruses in renal tissues of patients with IgAN. Enteroviruses have been reported as pathogenic agents in some renal diseases. We previously reported that group B coxsackieviruses cause pathological changes in experimentally infected mouse kidney. The aim of the present study was to examine the participation of enteroviruses in the pathogenesis of renal diseases including IgAN. Renal biopsies of ten patients with IgAN (group 1) and of 19 patients with non-IgAN renal disease (group 2) were analyzed by polymerase chain reaction (PCR) for the presence of enteroviral RNA. Positive PCR results were obtained for three patients (30%) of group 1. We confirmed by sequencing that the positive PCR products were derived from strains of enteroviruses. One of these three patients also had a positive result for lymphocytes from peripheral blood. In contrast, enteroviral RNA was detected in none of the 19 patients of group 2. The incidence of enteroviral RNA detection in patients of group 1 was higher than that in group 2 (P<0.05). Our findings suggest that enteroviral infection may have the possibility of becoming one of the factors involved in the mechanism of onset or evolution of IgAN.
authors.affiliation: Department of Pediatrics, Fukushima Medical University School of Medicine, Fukushima, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Takahashi
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Kawasaki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yoshida
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Mochizuki
authors.forename: Masato
authors.initials: M
authors.lastname: Isome
authors.forename: Ken
authors.initials: K
authors.lastname: Honzumi
authors.forename: Ruriko
authors.initials: R
authors.lastname: Nozawa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Suzuki
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Hosoya
authors.forename: Junzo
authors.initials: J
authors.lastname: Suzuki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D001706:Biopsy; D002648:Child; D004770:Enterovirus / Q000302:isolation & purification*; D005260:Female; D005922:Glomerulonephritis, IGA / Q000821:virology*; D006801:Humans; D007668:Kidney / Q000821:virology*; D008214:Lymphocytes / Q000821:virology; D008297:Male; D009393:Nephritis / Q000821:virology; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis
chemical_list: D012367:RNA, Viral