title: Regular arrangement of periodates bound to lysozyme.
abstract: The structure of tetragonal hen egg-white lysozyme soaked in a periodate solution has been determined to a resolution of 1.8 A. Four high-occupancy periodate positions have been identified on the basis of the anomalous signal of the I atoms. The four periodates exhibit a regular rectangular arrangement on the surface of the lysozyme molecule. No similar regular arrangement was found either in lysozyme crystals soaked in other heavy-atom anions or in other structures from the Protein Data Bank. Depending on their position on the surface of the protein, the periodate ions deviate to a varying extent from ideal octahedral geometry.
authors.affiliation: Department of Recombinant Expression and Structural Biology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo n. 2, CZ-16637 Praha 6, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Ondrácek
authors.forename: Manfred S
authors.initials: MS
authors.lastname: Weiss
authors.forename: Jirí
authors.initials: J
authors.lastname: Brynda
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Fiala
authors.forename: Frantisek
authors.initials: F
authors.lastname: Jursík
authors.forename: Pavlína
authors.initials: P
authors.lastname: Rezácová
authors.forename: Lasse B
authors.initials: LB
authors.lastname: Jenner
authors.forename: Juraj
authors.initials: J
authors.lastname: Sedlácek
mesh_terms: D000818:Animals; D002645:Chickens; D018360:Crystallography, X-Ray; D004527:Egg Proteins; D005260:Female; D006860:Hydrogen Bonding; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry*; D010504:Periodic Acid / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D004527:Egg Proteins; D010504:Periodic Acid; C009288:metaperiodate; D009113:Muramidase