title: Improvement of the lectin-antibody enzyme immunoassay of the alphafetoprotein carbohydrate chain for automation with the enzyme immunoassay robot.
abstract: The lectin-antibody enzyme immunoassay of the alphafetoprotein-L3 carbohydrate chain, a tumor marker of liver cancer, has not been automated. We improved the technique of the assay for automation. Consequently, alphafetoprotein-L3 and total alphafetoprotein were detected with two lectins using an automatic paramagnetic bead handling robot. This indicates that the improved method is potentially applicable to the automated enzyme immunoassay robot.
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tamano
authors.forename: Mika
authors.initials: M
authors.lastname: Sugiura
authors.forename: Jun
authors.initials: J
authors.lastname: Natsuki
authors.forename: Kazumi
authors.initials: K
authors.lastname: Sawakami-Kobayashi
authors.forename: Hideji
authors.initials: H
authors.lastname: Tajima
authors.forename: Masayuki
authors.initials: M
authors.lastname: Machida
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D012371:Robotics; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
chemical_list: D002241:Carbohydrates; D000509:alpha-Fetoproteins