LPS induces stefin A3 expression in mouse primary cultured glial cells.
We searched for a gene that is up-regulated in response to LPS at a later time point in primary cultured glial cells. Using a Gene Chip Probe Array, we identified stefin A3, which is known as a cysteine protease inhibitor. As assessed by RT-PCR, we observed a time-dependent (2 to 48 h) up-regulation of stefin A3. The results indicate that stefin A3 is involved in infection and inflammation at a later time point.
Hosoi
Toru
T
Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
Suzuki
Sawako
S
Okuma
Yasunobu
Y
Kawakami
Akiko
A
Ogawa
Naoki
N
Ozawa
Koichiro
K
Nomura
Yasuyuki
Y
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D055312:Cystatin A; D055313:Cystatin B; D015891:Cystatins / Q000235:genetics*; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis
C524992:Cstb protein, mouse; D055312:Cystatin A; D015891:Cystatins; D017931:DNA Primers; D008070:Lipopolysaccharides; C524998:Stfa3 protein, mouse; D055313:Cystatin B