Description of a simple multiplex PCR-SSCP method for AB0 genotyping and its application to the peopling of the Canary Islands.
A simple and affordable multiplex polymerase chain reaction-single-strand conformation polymorphism method is proposed for the molecular study of AB0 polymorphisms. Application of this method to the peopling of the Canary Islands, analyzing a total of 2,200 chromosomes, detected that in addition to Berbers and Basques, the rare alleles 0210 and O303 are also present in the Iberian Peninsula and in the Canary Islands. Allele B101, with the highest frequency in Northwest (NW) Africa, shows a negative correlation (R = -0.822, p = 0.023) between geographic distances from this continent and insular frequencies, congruent with a main aborigine colonization from East to West still detectable today. Similar to previous autosomal studies, admixture estimations point to a major Iberian contribution (82 +/- 0.5%) to the Canary Islands, although, in some islands as La Gomera, the NW African component raised to 62 +/- 4.3%.
Fregel
Rosa
R
Departamento de Genética, Facultad de BiologíaFacultad de Biología, Universidad de La Laguna, Tenerife, Spain.
Maca-Meyer
Nicole
N
Cabrera
Vicente Martínez
VM
González
Ana María
AM
Larruga
José María
JM
D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational
D000017:ABO Blood-Group System