A novel oscillating bioreactor BelloCell: implications for insect cell culture and recombinant protein production.
BelloCell is a novel packed bed bioreactor that allows alternating nutrient and gas transfer to a culture. Spodoptera frugiperda Sf-9 grown in the BelloCell (300 ml culture) reached 1.3-1.5x10(7) cells ml-1 in 7-8 days and the total baculovirus-expressed protein yield was 2.3-times that in a stirred tank bioreactor (600 ml culture). The superior cell and protein yields underline the potential of BelloCell for cell culture and recombinant protein production.
Lu
Jen-Te
JT
Department of Chemical Engineering, National Tsing Hua University, 300, Hsinchu, Taiwan.
Chung
Yao-Chi
YC
Chan
Zun-Ren
ZR
Hu
Yu-Chen
YC
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D019149:Bioreactors; D002452:Cell Count; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured / Q000737:chemistry; D005740:Gases; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007313:Insecta; D008856:Microscopy, Fluorescence; D009991:Oscillometry; D011994:Recombinant Proteins / Q000737:chemistry*; D013997:Time Factors
D005740:Gases; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins