[Explore on the advanced method of G-banding of Rana plancyt].
Using the technique of natural aging,chloroform and EDTA treatment,well-resolved G-banding patterns were successfully obtained in Rana plancyt's mitosis chromosome firstly. The G-banding patterns appeared distinctive. When analyzing some homologous chromosomes from the same metaphase figures and four macrochromosomes from different metaphase figures,the characteristic and the number of the bands were well matched. The method in this study is also economic and simple and the result is stable and reliable. The possible mechanism of G-banding was primarily discussed. The application of this method in the cytogenetics research of Rana plancyt and other amphibians is expected.
Qian
Shui-ming
SM
College of Life Sciences, Wuhan University, Wuhan 430072, China.
Yu
Qi-xing
QX