Polyvalent DNA prime and envelope protein boost HIV-1 vaccine elicits humoral and cellular responses and controls plasma viremia in rhesus macaques following rectal challenge with an R5 SHIV isolate.
Immunization of macaques with multivalent DNA encoding gp120 genes from HIV-1 subtypes A, B, C and E and a gag gene followed by boosting with homologous gp120 proteins elicited strong anti-gp120 antibodies capable of neutralizing homologous and to a lesser degree heterologous HIV-1 isolates. Both Env- and Gag-specific cell mediated immune (CMI) responses were detected in the immunized animals. Following rectal challenge with an SHIV isolate encoding HIV-1(Ba-L)env, plasma viremia in the infected immunized animals was significantly lower than that observed in the naïve animals. Further, one of six immunized animals was completely protected whereas all six naïve animals were infected. These results demonstrate that a vaccine based on priming with a polyvalent DNA vaccine from multiple HIV-1 subtypes followed by boosting with homologous Env proteins elicits anti-HIV-1 immune responses capable of controlling rectal transmission of SHIV(Ba-L).
Pal
Ranajit
R
Advanced BioScience Laboratories, Kensington, MD 20895, USA. ranajit.pal@ablinc.com
Wang
Shixia
S
Kalyanaraman
V S
VS
Nair
B C
BC
Whitney
Stephen
S
Keen
Timothy
T
Hocker
Lindsey
L
Hudacik
Lauren
L
Rose
Nicolas
N
Cristillo
Anthony
A
Mboudjeka
Innocent
I
Shen
Siyuan
S
Wu-Chou
Te-Hui
TH
Montefiori
David
D
Mascola
John
J
Lu
Shan
S
Markham
Phillip
P
D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015750:Genes, gag / Q000235:genetics / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology; D017958:Lentiviruses, Primate / Q000276:immunology; D008253:Macaca mulatta; D008992:Monkey Diseases / Q000276:immunology* / Q000821:virology*; D009000:Monocytes / Q000276:immunology; D009500:Neutralization Tests / Q000662:veterinary; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control*; D014766:Viremia / Q000662:veterinary
D016915:AIDS Vaccines; D000914:Antibodies, Viral; D015699:HIV Envelope Protein gp120; D007371:Interferon-gamma