title: Eukaryotic membrane protein overproduction in Lactococcus lactis.
abstract: Eukaryotic membrane proteins play many vital roles in the cell and are important drug targets. Approximately 25% of all genes identified in the genome are known to encode membrane proteins, but the vast majority have no assigned function. Although the generation of structures of soluble proteins has entered the high-throughput stage, for eukaryotic membrane proteins only a dozen high-resolution structures have been obtained so far. One major bottleneck for the functional and structural characterisation of membrane proteins is the overproduction of biologically active material. Recent advances in the development of the Lactococcus lactis expression system have opened the way for the high-throughput functional expression of eukaryotic membrane proteins.
authors.affiliation: The Medical Research Council, Dunn Human Nutrition Unit, Hills Road, Wellcome Trust/MRC Building, Cambridge CB2 2XY, United Kingdom. ek@mrc-dunn.cam.ac.uk
authors.forename: Edmund R S
authors.initials: ER
authors.lastname: Kunji
authors.forename: Ka Wai
authors.initials: KW
authors.lastname: Chan
authors.forename: Dirk Jan
authors.initials: DJ
authors.lastname: Slotboom
authors.forename: Suzanne
authors.initials: S
authors.lastname: Floyd
authors.forename: Rosemary
authors.initials: R
authors.lastname: O'Connor
authors.forename: Magnus
authors.initials: M
authors.lastname: Monné
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005057:Eukaryotic Cells / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D013294:Lactococcus lactis / Q000235:genetics*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D026901:Membrane Transport Proteins / Q000096:biosynthesis / Q000235:genetics; D000226:Mitochondrial ADP, ATP Translocases / Q000096:biosynthesis / Q000235:genetics; D018000:Receptors, Peptide / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: C064459:KDEL receptor; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C112225:PET9 protein, S cerevisiae; D018000:Receptors, Peptide; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D000226:Mitochondrial ADP, ATP Translocases