title: Improvement of phenylethanoid glycosides biosynthesis in Cistanche deserticola cell suspension cultures by chitosan elicitor.
abstract: Effect of chitosan elicitor on growth and phenylethanoid glycosides (PeGs) accumulation in Cistanche deserticola cell suspension cultures was investigated. PeGs accumulation was dramatically improved by addition of selected chitosan at optimal elicitation conditions. Furthermore, a strategy of repeated addition of the chitosan elicitor for enhancing PeGs accumulation was developed. The chitosan elicitor of 10 mg l(-1)-medium repeatedly added on days 15 and 17 improved PeGs accumulation further, and the final PeGs production in the treated cell cultures of C. deserticola reached 364.6 mg l(-1), which was 3.4-fold higher than that of the control without elicitation. The increase of PeGs accumulation in C. deserticola cell suspension cultures was related to the increase of phenylalanine ammonium lyase activity stimulated by the chitosan elicitor.
authors.affiliation: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, PR China.
authors.forename: Xi-Yu
authors.initials: XY
authors.lastname: Cheng
authors.forename: Hua-Ying
authors.initials: HY
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Cui
authors.forename: Wen
authors.initials: W
authors.lastname: Ni
authors.forename: Chun-Zhao
authors.initials: CZ
authors.lastname: Liu
mesh_terms: D019149:Bioreactors; D002614:Chelating Agents / Q000494:pharmacology*; D048271:Chitosan / Q000494:pharmacology*; D031671:Cistanche / Q000166:cytology / Q000378:metabolism*; D006027:Glycosides / Q000096:biosynthesis*
chemical_list: D002614:Chelating Agents; D006027:Glycosides; D048271:Chitosan