[Expression of human insulin gene in vitro and in vivo].
The transfection of mutated human insulin gene was studied using the complex of different proportion of plasmid and Liposome. Hepatic cell was used as the target cell in vitro,isolation of Hepatic cell including insulin gene was carried out by G418, the expression level of insulin in medium was measured by RIA method. The portal vein was cannulated with therapeutic gene in vivo, the blood glucose of the model was regularly examined and the insulin level was detected on the seventh day after transfection. The results showed that the target gene was transferred into the hepatic cell, expression of mature insulin was detected both in vivo and in vitro. It reached the peak 10.45microIU/ml on the 24th hour after transfection with the proportion 1:6 of plasmid and Liposome in hepatic cell. Diabetic symptom of the model was improved after transgene, the blood glucose could decrease 55% at the most.
Cao
Yue-qing
YQ
Biotechnology Center, Yi Ling Pharmacy Institute, Shijiazhuang, China.
Zhou
Ling
L
Han
Zhu
Z
Shang
Lei-xiong
LX
Hu
Liang-ju
LJ
Yao
Wen-bin
WB