title: [Expression of the heat shock protein-70 in the myocardial cells of cyanosis congenital heart diseases].
abstract: OBJECTIVE
  To evaluate the expression and myocardial protection of heat shock protein-70 (HSP70) in the myocardial cells of cyanosis congenital heart diseases.
  
  
  METHODS
  The study enrolled 24 patients with congenital heart disease: 12 cases of non-cyanosis (group A), and the other 12 cases of cyanosis (group B). During the operation the samples of myocardium in the right atrium were attained at 0 min ischemia, 20 min ischemia, and 20 min reperfusion. They were used to evaluate the content of HSP70 by immunohistochemistry and activation of SOD and content of MDA by chemochromatometry.
  
  
  RESULTS
  The content of HSP70 in myocardial cells during the operation was not significantly different in both groups (P > 0. 05). The content of HSP70 significantly decreased in group B compared with that of group A (P <0.05). The activity of SOD significantly decreased and the content of MDA significantly increased in group B during the operation (P < 0.05).
  
  
  CONCLUSIONS
  Decreased expression of HSP70 is associated with the decreased tolerance to ischemia-reperfusion injury in myocardial cells of cyanosis congenital heart disease.
authors.affiliation: Department of Cardiothoracic Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Shi-Bing
authors.initials: SB
authors.lastname: Wen
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Hu
authors.forename: Zhong-Xia
authors.initials: ZX
authors.lastname: Ma
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Zhou
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Yang
authors.forename: Feng-Lei
authors.initials: FL
authors.lastname: Yu
authors.forename: Min-Song
authors.initials: MS
authors.lastname: Hu
mesh_terms: D004310:Double Outlet Right Ventricle / Q000378:metabolism*; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D013771:Tetralogy of Fallot / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D013482:Superoxide Dismutase