title: A proteomic approach to study protein variation in GM durum wheat in relation to technological properties of semolina.
abstract: Genetic manipulation of durum wheats by tobacco rab-1 genes influence the trafficking of gluten proteins through the secretory system by up- or down-regulating the transport step from the ER to the Golgi apparatus which may in turn modify functional performance of the grain. Gluten proteins were extracted from two genetically manipulated lines - Svevo B730 1-1 and Ofanto B688 1-2 - and their control lines and were analyzed by two dimensional gel electrophoresis. When the two-dimensional maps were compared by image analysis no significant differences between the GM line with an up-regulated trafficking containing the wild type tobacco rab1 (Svevo B730 1-1) and its control (Svevo control). By contrast, significant differences were found between the GM line with a down-regulated trafficking due to the tobacco rab1 mutant form (Ofanto B688 1-2) and its control (Ofanto control). Of the new protein spots detected in the down-regulated Ofanto B688 1-2 map, only a beta-amylase was identified. The remaining spots were susceptible to chymotripsin action but not to trypsin one, as in the case of the gluten protein. Rheological measurements showed that gluten quality was enhanced in the down-regulated Ofanto B688 1-2 without an increase in the amount of gluten. Proteomics is a useful and powerful tool for investigating protein changes in GMOs and in understanding events in food science and technology.
authors.affiliation: Dipartimento di Produzioni Animali, Università di Bari, via Amendola 156/A, Bari, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Di Luccia
authors.forename: Carmela
authors.initials: C
authors.lastname: Lamacchia
authors.forename: Clara
authors.initials: C
authors.lastname: Fares
authors.forename: Lucia
authors.initials: L
authors.lastname: Padalino
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Mamone
authors.forename: Barbara
authors.initials: B
authors.lastname: La Gatta
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gambacorta
authors.forename: Michele
authors.initials: M
authors.lastname: Faccia
authors.forename: Natale
authors.initials: N
authors.lastname: Di Fonzo
authors.forename: Ennio
authors.initials: E
authors.lastname: La Notte
mesh_terms: D015536:Down-Regulation; D005524:Food Technology; D005983:Glutens / Q000096:biosynthesis* / Q000378:metabolism; D030821:Plants, Genetically Modified; D040901:Proteomics; D014908:Triticum / Q000235:genetics*; D015854:Up-Regulation; D020691:rab GTP-Binding Proteins / Q000235:genetics*
chemical_list: D005983:Glutens; D020691:rab GTP-Binding Proteins