Pneumococci: drug susceptibilities and preliminary epidemiological studies by penicillin binding protein genotyping.
A collection of 307 pneumococcal isolates form 84 children and 223 adults admitted to Siriraj Hospital were separated into two groups, penicillin-susceptible (PSSP) and penicillin-nonsusceptible (PNSP). Each group was tested for susceptibilities to 12 drugs (cefuroxime, amoxicillin, chloramphenicol, tetracycline, cefotaxime, ceftriaxone, imipenem, meropenem, ciprofloxacin, ofloxacin, erythromycin and co-trimoxazole). PSSP were susceptible to cefuroxime (87.5%), amoxicillin (100%), chloramphenicol (84.7%), tetracycline (45.8%), cefotaxime (99%), ceftriaxone (99%), imipenem (99%), meropenem (100%), ciprofloxacin (76%), ofloxacin (99%), erythromycin (94.8%) and co-trimoxazole (61.5%). PNSP were resistant to most drugs, except for amoxicillin (99%), ofloxacin (99%) and ciprofloxacin (86.3%). Twenty-two pneumococcal isolates belonging to the three most common serotypes (6, 19, 23) were randomly selected for studies of the pbp2b gene with RFLP. There were 7 distinct pbp2b RFLP patterns. RFLP pattern 1 was the most predominant resistant pattern. The RFLP pattern 2 was found only in PSSP.
Srifeungfung
Somporn
S
Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, 2 Prannok Rd, Bangkok 10700, Thailand. sissf@mahidol.ac.th
Thammawart
Sutiwan
S
Leelarasamee
Amorn
A
Chokephaibulkit
Kulkanya
K
Dejsirilert
Surang
S
Tribuddharat
Chanwit
C
D000293:Adolescent; D000328:Adult; D019881:Aminoacyltransferases / Q000235:genetics*; D002648:Child; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D015378:Imipenem / Q000494:pharmacology / Q000627:therapeutic use; D000077731:Meropenem; D008875:Middle Aged; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D010406:Penicillins / Q000494:pharmacology* / Q000627:therapeutic use; D011008:Pneumococcal Infections / Q000188:drug therapy* / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics*; D013785:Thailand / Q000453:epidemiology; D013845:Thienamycins / Q000494:pharmacology / Q000627:therapeutic use
D046915:Penicillin-Binding Proteins; D010406:Penicillins; D013845:Thienamycins; D015378:Imipenem; D019881:Aminoacyltransferases; C478701:penicillin-binding protein 2b, Streptococcus; D000077731:Meropenem