Changes in the gene expression profiling of the thymus in response to fibrosarcoma growth.
The aim of this study was to determine whether subcutaneous injection of tumor cells into Balb-c mice, which induces a fibrosarcoma at the site of injection, produced a differential expression profile in the thymus that could be correlated with tumor growth. A dynamic transcriptional profile of the thymus in response to tumor development was observed using nylon cDNA microarrays. The Cluster-Tree View and the SAM programs were used to reveal induced and repressed genes during tumor growth. This experimental model-system showed that this approach is adequate to detect the presence of tumor cells in vivo.
Marques
Márcia M C
MM
Molecular Immunogenetics Group, Department of Genetics, Faculty of Medicine, University of São Paulo (USP), 14040-900, Ribeirão Preto, SP, Brazil.
Junta
Cristina M
CM
Cardoso
Renato S
RS
Mello
Stephano S
SS
Sakamoto-Hojo
Elza T
ET
Donadi
Eduardo A
EA
Passos
Geraldo A S
GA
D000626:Aminopeptidases / Q000235:genetics; D000818:Animals; D005260:Female; D005354:Fibrosarcoma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D013950:Thymus Gland / Q000378:metabolism*
D000626:Aminopeptidases