title: 2-O-(2-hydroxybutyl)-beta-cyclodextrin as a chiral selector for the capillary electrophoretic separation of chiral drugs.
abstract: A new beta-cyclodextrin (beta-CD) derivative, 2-O-(2-hydroxybutyl)-beta-CD (HB-beta-CD), was successfully synthesized and used as chiral selector in capillary zone electrophoresis. Six chiral drugs, such as anisodamine, ketoconazole, propranolol, promethazine, adrenaline and chlorphenamine enantiomers, belonging to different classes of compounds of pharmaceutical interest were resolved. The chiral resolution (R(S)) was strongly influenced by the concentrations of the cyclodextrin derivative, the background electrolyte, and the pH of the background electrolyte. Under the conditions of 50 mmol/L tris-phosphate buffer at pH 2.5 containing 5 mmol/L 2-O-(2-hydroxybutyl)-beta-CD, the baseline separation of enantiomers, such as anisodamine (R(S) = 3.10), ketoconazole (R(S) = 3.01), propranolol (R(S) = 3.87), promethazine (R(S) = 3.63), adrenaline (R(S) = 3.42) and chlorphenamine (R(S) = 2.96), could be achieved.
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Wei
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Chenfu
authors.initials: C
authors.lastname: Zhu
authors.forename: Aiyou
authors.initials: A
authors.lastname: Hao
authors.forename: Minggang
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000737:chemistry; D000319:Adrenergic beta-Antagonists / Q000737:chemistry; D000935:Antifungal Agents / Q000737:chemistry; D002744:Chlorpheniramine / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D004837:Epinephrine / Q000737:chemistry; D006634:Histamine H1 Antagonists / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007654:Ketoconazole / Q000737:chemistry; D008968:Molecular Conformation; D015394:Molecular Structure; D011398:Promethazine / Q000737:chemistry; D011433:Propranolol / Q000737:chemistry; D012991:Solanaceous Alkaloids / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: C507312:2-O-(2-hydroxybutyl)-beta-cyclodextrin; D000316:Adrenergic alpha-Agonists; D000319:Adrenergic beta-Antagonists; D000935:Antifungal Agents; D006634:Histamine H1 Antagonists; D012991:Solanaceous Alkaloids; D047392:beta-Cyclodextrins; C003922:anisodamine; D002744:Chlorpheniramine; D011433:Propranolol; D011398:Promethazine; D007654:Ketoconazole; D004837:Epinephrine