title: Silver-ion reversed-phase comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection in lipidic food analysis.
abstract: The triacylglycerol (TAG) profiles present in real world lipidic samples are usually quite complex and, as such, monodimensional high-performance liquid chromatographic (HPLC) techniques are inadequate when challenged with such matrices. In this respect, the complementary use of silver-ion (Ag) and non-aqueous (NA) reversed-phase (RP) HPLC can be exploited if thorough TAG separations are required. The present investigation reports the employment of a newly developed comprehensive LC (LC x LC) system, based on the different separation mechanisms of the aforementioned techniques, and applied to a rice oil sample. The approach was successful in the separation of a high number of solutes, otherwise unachievable through monodimensional LC. Furthermore, the use of atmospheric pressure chemical ionization mass spectrometry (APCI-MS), as detection system, provided a third analytical dimension boosting the identification power of the comprehensive chromatographic method.
authors.affiliation: Dipartimento Farmaco-Chimico, Facoltá di Farmacia, Universitá di Messina, viale Annunziata, 98168 Messina, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Mondello
authors.forename: P Q
authors.initials: PQ
authors.lastname: Tranchida
authors.forename: Vaclav
authors.initials: V
authors.lastname: Stanek
authors.forename: Pavel
authors.initials: P
authors.lastname: Jandera
authors.forename: G
authors.initials: G
authors.lastname: Dugo
authors.forename: P
authors.initials: P
authors.lastname: Dugo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis; D008055:Lipids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D008055:Lipids