Characterization of caprine herpesvirus 1 glycoprotein D gene and its translation product.
Caprine herpesvirus 1 (CpHV-1) is responsible of systemic infection in neonatal kids as well as abortion and fertility disorders in adult goats. This virus is closely related to bovine herpesvirus 1 (BoHV-1) which causes infectious bovine rhinotracheitis. Glycoprotein D (gD) mediates important functions in alphaherpesviruses and is also a main immunogen. The sequence of CpHV-1 gD gene and the biochemical properties of its translation product were analyzed and compared to those of BoHV-1 and other alphaherpesviruses. A relatively high homology was found between CpHV-1 and BoHV-1 glycoproteins D amino acid sequences (similarity of 68.8%). Moreover, six cysteine residues are conserved by CpHV-1 gD and the other studied alphaherpesviruses. CpHV-1 gD has a molecular mass similar to BoHV-1 gD and contains complex N-linked oligosaccharides. In contrast to the BoHV-1 gD, CpHV-1 gD is expressed as a late protein. In spite of the observed differences which could influence its biological functions, CpHV-1 gD shares most characteristics with other alphaherpesviruses and especially BoHV-1.
Keuser
Véronique
V
Department of Infectious and Parasitic Diseases, Virology and Immunology, Faculty of Veterinary Medicine, University of Liège, B-4000 Liège, Belgium.
Detry
Bruno
B
Thiry
Julien
J
de Fays
Katalin
K
Schynts
Frédéric
F
Pastoret
Paul-Pierre
PP
Vanderplasschen
Alain
A
Thiry
Etienne
E
D018138:Alphaherpesvirinae / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D017124:Conserved Sequence; D003545:Cysteine / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D018140:Varicellovirus / Q000737:chemistry* / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics*
D004279:DNA, Viral; D006023:Glycoproteins; D009844:Oligosaccharides; D014759:Viral Envelope Proteins; D003545:Cysteine