title: [Effect of bizhongxiao decotion on TNF-alpha and interleukin-1beta in plasma of rats with C II-induced rheumatoid arthritis].
abstract: OBJECTIVE
  To observe the influence of bizhongxiao decotion (BZXD) on the plasma TNF-alpha and IL-1beta in rats with C II-induced arthritis (CIA) and to explore the mechanism of BZXD in the treatment of rheumatoid arthritis.
  
  
  METHODS
  We divided 75 rats into 4 groups randomly. The rat experimented arthritis model was established by subcutaneous injection with collagen II. The plasma TNF-alpha and IL-1beta levels were detected with the radio-immunity assay at different time spots.
  
  
  RESULTS
  The incidence of arthritis in the rats immunized with C II was approximately 88%. The plasma TNF-alpha and IL-1beta levels of the model group, BZXD group and methotrexate (MTX) group were notably higher than those of the normal group (P < 0.05). The plasma TNF-alpha and IL-1beta levels of the model group were higher than those of the MTX control group and BZXD treatment group at different time spots (P < 0. 01). The plasma TNF-alpha and IL-1beta levels rose step by step, but those of the BZXD group and MTX group decreased gradually. Moreover, the plasma TNF-alpha and IL-1beta levels of the rats of BZXD group were lower than those of the MTX group (P < 0. 05).
  
  
  CONCLUSIONS
  TNF-alpha and IL-1beta play a very important role in the formation and development of rheumatoid arthritis. Both BZXD and MTX can notably decrease the plasma TNF-alpha and IL-1beta levels, but the effect of BZXD is better than that of MTX.
authors.affiliation: Institute of Combined Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: An-Yu
authors.initials: AY
authors.lastname: Wang
authors.forename: Qing-Hu
authors.initials: QH
authors.lastname: Liang
authors.forename: Jiang
authors.initials: J
authors.lastname: Chen
authors.forename: Xu
authors.initials: X
authors.lastname: Luo
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Li
authors.forename: Da-Cheng
authors.initials: DC
authors.lastname: Bao
authors.forename: Tian-Feng
authors.initials: TF
authors.lastname: Tang
authors.forename: Yong
authors.initials: Y
authors.lastname: Tan
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000139:chemically induced; D024043:Collagen Type II; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007375:Interleukin-1 / Q000097:blood*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D024043:Collagen Type II; D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha