[Effect of interleukin-12 on HBV pan-S gene vaccine].
OBJECTIVE
To investigate the effect of interleukin-12 (IL-12) on immune responses induced by pcDNA3 which expresses hepatitis B virus pan-S gene.


METHODS
BABL/c mice were immunized with pcDNA3 alone (control group), pcDNA3-PS alone, or co-immunized with pcDNA3-PS and pcDNA3-IL12. Six weeks after the immunization, sero anti-HBS antibody was examined, lymphocyte proliferation test and specific cytokine level were assayed. RESULTS The titer of anti-HBs antibody, the stimulation index of splenocytes, and cytokine level of IL-12 and IFN-gamma from the mice coimmunized with pcDNA3-PS and pcDNA3-IL12 were significantly higher than those of the mice immunized alone with pcDNA3-PS. Antibody level, SI, and cytokine level of the immunized mice were higher than those of the control group.


CONCLUSIONS
IL-12 expression plasmid can act as immunological adjuvants for HBV pan-S gene vaccine.
Tan
De-Ming
DM
Institute of Infectious Disease, Xiangya Hospital, Central South University, Changsha 410008, China.
Yang
Yong-Feng
YF
Liu
Shui-Ping
SP
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D006510:Hepatitis B Antibodies / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics*; D018664:Interleukin-12 / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000276:immunology*
D000276:Adjuvants, Immunologic; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D019444:Vaccines, DNA; D018664:Interleukin-12