title: Deposition and depletion of five anticoccidials in eggs.
abstract: Anticoccidials are compounds that are widely used as feed additives to prevent and treat coccidiosis. They are licensed for use in a prescribed concentration and during a certain time interval for broilers and pullets but not for laying hens. It was shown in the past that carry-over at the feeding mill is found to be the main reason for the presence of residues in eggs. An animal experiment was set up to investigate the effect of carry-over at the feeding mill on the presence of residues of anticoccidials in eggs. For the compounds diclazuril, robenidine, halofuginone and nicarbazin in combination with narasin, two concentration levels were tested: the maximum allowed concentration for broilers (100%) and a concentration corresponding to 5% carry-over during feed preparation. Also dimetridazole was included in the experiment but only at one concentration level. Eggs were sampled during treatment (14 days) and for a period of 30 days after withdrawal of the anticoccidial-containing feed. Residues were determined, and deposition and depletion curves were generated. Analyses were performed by ELISA and LC-MS/MS. For all compounds, substantial residues could be found in the 5% groups, which points out the risk of carry-over at the feeding mill. The distribution of the residues between egg yolk and white was determined by analyzing both fractions.
authors.affiliation: Ministry of the Flemish Community, Agricultural Research Centre Ghent (CLO), Department of Animal Product Quality and Transformation Technology (DVK), Brusselsesteenweg 370, 9090 Melle, Belgium. L.Mortier@clo.fgov.be
authors.forename: Leen
authors.initials: L
authors.lastname: Mortier
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Huet
authors.forename: Els
authors.initials: E
authors.lastname: Daeseleire
authors.forename: Gerard
authors.initials: G
authors.lastname: Huyghebaert
authors.forename: Terrence
authors.initials: T
authors.lastname: Fodey
authors.forename: Christopher
authors.initials: C
authors.lastname: Elliott
authors.forename: Philippe
authors.initials: P
authors.lastname: Delahaut
authors.forename: Carlos
authors.initials: C
authors.lastname: Van Peteghem
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002645:Chickens; D003049:Coccidiostats / Q000008:administration & dosage* / Q000032:analysis* / Q000493:pharmacokinetics; D004529:Egg White / Q000032:analysis; D004530:Egg Yolk / Q000737:chemistry; D004531:Eggs / Q000032:analysis*; D005260:Female; D007700:Kinetics
chemical_list: D003049:Coccidiostats