Photochemical patterning of biological molecules inside a glass capillary.
A simple way for photochemical patterning of biological molecules onto the inner wall of fused-silica capillary is described. The method is based on a modification of the inner capillary surface with photoactive benzophenone (BP) derivative. The UV irradiation at 365 nm of the capillary filled with a sample solution results in cross-linking of the solutes to the BP moiety via a stable covalent bond. As a proof of concept, oligonucleotides and proteins were arrayed inside the capillary using an inverted microscope as an irradiation device. We demonstrated that the capillary arrays produced in this way are functional and could be used in different bioassays including DNA hybridization, protein interaction studies, and immunoassays. Having a sensitivity comparable to the fluorophore-based assays in a planar format, the capillary array possesses several advantages including submicroliter sample volume and a short assay time. The capillary format should therefore be considered as a possible alternative to a planar format in a number of low-density array applications such as mutation detection and diagnostic immunoassays.
Balakirev
Maxim Y
MY
Laboratoire Biopuces, Département Réponse et Dynamique Cellulaires, and Laboratoire d'Electronique de Technologie de l'Information (LETI), Commissariat à l'Energie Atomique, 17 rue des Martyrs, 38054 Grenoble, France. mbalakirev@cea.fr
Porte
Stéphanie
S
Vernaz-Gris
Maud
M
Berger
Michel
M
Arié
Jean-Philippe
JP
Fouqué
Brigitte
B
Chatelain
François
F
D001577:Benzophenones / Q000737:chemistry; D002197:Capillary Action; D005898:Glass / Q000737:chemistry*; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry; D040081:Protein Array Analysis; D013499:Surface Properties
D001577:Benzophenones; D009841:Oligonucleotides; C047723:benzophenone