title: Tumor-immune memory T cells from the bone marrow exert GvL without GvH reactivity in advanced metastasized cancer.
abstract: The bone marrow has been shown to represent a unique microenvironment where T cell immune responses against tumor associated antigens can be initiated and tumor-immune memory T cells are enriched. In the present study the graft versus leukemia (GvL) reactivity of bone marrow derived tumor-immune memory T cells was analyzed in an allogeneic minor histocompatibility different murine GvL tumor model with late stage disease. A single adoptive cell transfer of B10.D2 donor immune bone marrow cells (iBM) into sub-lethally irradiated late stage ESb-MP tumor-bearing DBA/2 mice led to a complete tumor remission and to significant life prolongation. Even though the frequency of bone marrow resident T cells is only around 2%, the GvL reactivity of iBM was superior to immune cells from the spleen (iSPL) or to those from the peritoneal cavity (iPEC) of the same immunized donors. iPEC exerted mostly unwanted graft versus host (GvH) reactivity, while iSPL exerted GvL and GvH reactivity. Bone marrow cells from naive donor mice or T cell depleted iBM cells were completely devoid of GvL reactivity. The low number of tumor-immune memory T cells thus conferred the GvL reactivity of iBM cells.
authors.affiliation: German Cancer Research Center, Division of Cellular Immunology, D-69120 Heidelberg, Germany. v.schirrmacher@dkfz-heidelberg.de
authors.forename: Volker
authors.initials: V
authors.lastname: Schirrmacher
authors.forename: Philipp
authors.initials: P
authors.lastname: Beckhove
authors.forename: Carmen
authors.initials: C
authors.lastname: Choi
authors.forename: Andreas
authors.initials: A
authors.lastname: Griesbach
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Mahnke
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D006087:Graft vs Host Reaction; D007107:Immune System / Q000378:metabolism; D007156:Immunologic Memory; D007700:Kinetics; D051379:Mice; D008811:Mice, Inbred DBA; D009362:Neoplasm Metastasis; D010641:Phenotype; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013997:Time Factors