title: A combined nucleocapsid vaccine induces vigorous SARS-CD8+ T-cell immune responses.
abstract: Several studies have shown that cell-mediated immune responses play a crucial role in controlling viral replication. As such, a candidate SARS vaccine should elicit broad CD8+ T-cell immune responses. Several groups of mice were immunized alone or in combination with SARS-nucleocapsid immunogen. A high level of specific SARS-CD8+ T-cell response was demonstrated in mice that received DNA encoding the SARS-nucleocapsid, protein and XIAP as an adjuvant. We also observed that co-administration of a plasmid expressing nucleocapsid, recombinant protein and montanide/CpG induces high antibody titers in immunized mice. Moreover, this vaccine approach merits further investigation as a potential candidate vaccine against SARS.
authors.affiliation: Infectious Disease and Vaccine Research Centre, Children's Hospital of Eastern Ontario Research Institute, Ottawa, ON, K1H 8L1, Canada. aliazizi555@yahoo.ca
authors.forename: Ali
authors.initials: A
authors.lastname: Azizi
authors.forename: Susan
authors.initials: S
authors.lastname: Aucoin
authors.forename: Helina
authors.initials: H
authors.lastname: Tadesse
authors.forename: Rita
authors.initials: R
authors.lastname: Frost
authors.forename: Masoud
authors.initials: M
authors.lastname: Ghorbani
authors.forename: Catalina
authors.initials: C
authors.lastname: Soare
authors.forename: Turaya
authors.initials: T
authors.lastname: Naas
authors.forename: Francisco
authors.initials: F
authors.lastname: Diaz-Mitoma