A comparison between hairy root cultures and wild plants of Saussurea involucrata in phenylpropanoids production.
Saussurea involucrata is an important medicinal plant that produces a few bioactive secondary metabolites, such as hispidulin, rutin, and syringin. Previously, we established a hairy root culture system for this species through Agrobacterium-mediated transformation. The present study addressed the issue as how hairy root cultures perform in phenylpronoid accumulation. From the ethanolic extract of a hairy root culture established for Saussurea involucrata, syringin, rutin and hispidulin, were isolated and their chemical structures were confirmed by HPLC-ESI-MS. A quantitative study of the compounds showed great levels of syringin and hispidulin (being 43.5+/-1.13 and 0.34+/-0.023 mg g-1 dry weight, respectively), about 40 and 3 times, respectively, higher than those from wild plants. But, the levels of rutin from hairy roots were much lower (0.71+/-0.043 vs. 6.59+/-0.56 mg g-1 dry weight). Compared with untransformed root cultures, syringin and hispidulin levels were also higher. An experiment on culture media showed that MS was superior to others for phenylpropanoids accumulation in hairy roots, a 28-day culture produced 405 mg l-1 syringin.
Fu
Chun-Xiang
CX
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing, 100093,, P.R., China.
Xu
Yan-jun
YJ
Zhao
De-Xiu
DX
Ma
Feng Shan
FS
D002851:Chromatography, High Pressure Liquid; D010666:Phenylpropionates / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D012015:Reference Standards; D030441:Saussurea / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
D010666:Phenylpropionates