Excitation mapping with the organic cation AGB2+.
Excitation mapping is a method of visualizing the signaling history of neurons with permeant organic cations. It is compatible with high-resolution imaging, allowing concurrent visualization of all neuronal classes and their glutamate-gated excitation histories. Excitation mapping documents the stability and precision of neuronal signaling within a given neuronal class, arguing that single unit electrophysiological sampling accurately reflects neuronal diversity. We here review the theory of excitation mapping, provide methods and protocol links; outline imaging concepts; provide parametric data on the temporal range and physiological sensitivity of excitation mapping; and show that immunocytochemical methods for macromolecules are compatible with excitation mapping.
Marc
Robert E
RE
John A. Moran Eye Center, University of Utah School of Medicine, Salt Lake City, 84132, USA. robert.marc@hsc.utah.edu
Kalloniatis
Michael
M
Jones
Bryan W
BW
D000376:Agmatine / Q000737:chemistry / Q000493:pharmacokinetics*; D000818:Animals; D002412:Cations; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D018698:Glutamic Acid / Q000494:pharmacology*; D007150:Immunohistochemistry; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
D002412:Cations; D018691:Excitatory Amino Acid Antagonists; D018698:Glutamic Acid; D000376:Agmatine