Suppression of expression of muscle-associated proteins by PPARalpha in brown adipose tissue.
Peroxisome proliferator-activated receptor alpha (PPARalpha) belongs to the steroid/nuclear receptor superfamily. Two-dimensional (2D) SDS-PAGE analysis of brown adipose tissue (BAT) unexpectedly revealed six spots that were present only in PPARalpha-null mice. Proteomic analysis indicated that these proteins were tropomyosin-1 alpha chain, tropomyosin beta chain, myosin regulatory light chain 2, myosin light chain 3, and parvalbumin alpha. Analyses of mRNA have revealed that PPARalpha suppressed the genes encoding these proteins in a synchronous manner in adult wild-type mice. Histological and physiological analyses of BAT showed in adult wild-type mice, a marked suppression of BAT growth concurrent with a prominent decrease in lipolytic and thermogenesis activities. These results suggest that in adult mice, PPARalpha functions to suppress the expression of the proteins that may be involved in the architecture of BAT, and thus may function in keeping BAT in a quiescent state.
Tong
Yuhong
Y
Department of Metabolic Regulation, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
Hara
Atsushi
A
Komatsu
Makiko
M
Tanaka
Naoki
N
Kamijo
Yuji
Y
Gonzalez
Frank J
FJ
Aoyama
Toshifumi
T
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D008066:Lipolysis; D051379:Mice; D018345:Mice, Knockout; D009124:Muscle Proteins / Q000235:genetics*; D047493:PPAR alpha / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D017931:DNA Primers; D009124:Muscle Proteins; D047493:PPAR alpha; D012333:RNA, Messenger