title: Detection of human papillomavirus DNA in benign and malignant sinonasal neoplasms.
abstract: Infections with human papillomaviruses are divided basically into three different infection types: those producing specific clinically visible lesions, those remaining subclinical, and those being latent. The assumed infection type thought to be present in tissue specimens has influence on the conclusions that can be made from an analysis, i.e. whether or not the HPV infection has a causal relationship with other epidemiological or molecular investigation observations. To determine whether HPV DNA detection in different entities of the upper aerodigestive tract represents a coincidental, persistent/latent or specific infection, 20 clinically intact mucosa specimens of the upper aerodigestive tract, 20 sinonasal polyps, 26 inverted papillomas, and 20 squamous cell carcinomas of the paranasal sinuses were investigated. HPV DNA was not detectable in specimens derived from clinically intact mucosa or in nasal polyps. Yet, three out of 26 inverted papillomas were HPV-positive, each showing double infection with HPV6 and 11. Four out of 20 squamous cell carcinomas were HPV16 positive. To our knowledge, we are presenting the first study contemporaneously analyzing benign as well as malignant non-proliferative and proliferative mucosal entities whilst applying identical methodical standards. The data corroborate the hypothesis that HPV DNA demonstration in tissue specimens represents a specific infection of the mucosa of the upper aerodigestive tract. It can thus be assumed that there is a causative involvement of HPV infections in the alteration of cell proliferation and in the case of infection with high risk HPV types even on progression to malignant transformation.
authors.affiliation: Klinik für Hals-, Nasen-, und Ohrenheilkunde, Kopf- und Halschirurgie, Christian-Albrechts-Universität Kiel, Arnold-Heller-Street 14, D-24105 Kiel, Germany. mhoffmann@hno.uni-kiel.de
authors.forename: Markus
authors.initials: M
authors.lastname: Hoffmann
authors.forename: Nina
authors.initials: N
authors.lastname: Klose
authors.forename: Stefan
authors.initials: S
authors.lastname: Gottschlich
authors.forename: Tibor
authors.initials: T
authors.lastname: Görögh
authors.forename: Asita
authors.initials: A
authors.lastname: Fazel
authors.forename: Claudia
authors.initials: C
authors.lastname: Lohrey
authors.forename: Werner
authors.initials: W
authors.lastname: Rittgen
authors.forename: Petra
authors.initials: P
authors.lastname: Ambrosch
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Schwarz
authors.forename: Tomas
authors.initials: T
authors.lastname: Kahn
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane; D009296:Nasal Cavity / Q000473:pathology / Q000821:virology; D009298:Nasal Polyps / Q000473:pathology / Q000821:virology; D018308:Papilloma, Inverted / Q000473:pathology / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D010255:Paranasal Sinus Neoplasms / Q000473:pathology / Q000821:virology*; D010256:Paranasal Sinuses / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral