title: Ascorbic acid and the oxidative processes in pea root cell wall isolates: characterization by fluorescence and EPR spectroscopy.
abstract: A comparative fluorescence and oxygen radical-sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H(2)O(2) is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen-dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals.
authors.affiliation: Center for Multidisciplinary Studies, University of Belgrade, Serbia and Montenegro. sonjajov@ibiss.bg.ac.yu
authors.forename: Sonja
authors.initials: S
authors.lastname: Veljović-Jovanović
authors.forename: Biljana
authors.initials: B
authors.lastname: Kukavica
authors.forename: Tijana
authors.initials: T
authors.lastname: Cvetić
authors.forename: Milos
authors.initials: M
authors.lastname: Mojović
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Vucinić
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D018532:Pisum sativum / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D011758:Pyrroles / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D019097:Spin Trapping
chemical_list: C510912:(diethoxyphosphoryl)-5-methylpyrroline N-oxide; D010636:Phenols; D011506:Proteins; D011758:Pyrroles; D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid; D010100:Oxygen