Increased expression of S100A6 is associated with decreased metastasis and inhibition of cell migration and anchorage independent growth in human osteosarcoma.
While most osteosarcoma patients have metastatic or micrometastatic lesions, less than 15% of them have clinically detectable metastatic diseases at presentation. To identify potential markers that may predict osteosarcoma metastasis, we analyzed the expression of S100A6 in 50 osteosarcoma cases and found that 84% of the analyzed specimens stained positive for S100A6. There is a trend towards decreased clinically evident metastasis with increased S100A6 staining. Overexpression of S100A6 in osteosarcoma cells decreases cell motility and anchorage independent growth on collagen gels. Our findings provide evidence that, while S100A6 is commonly overexpressed in human osteosarcoma, loss of its expression correlates with a metastatic phenotype.
Luu
Hue H
HH
Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, IL 60637, USA.
Zhou
Lan
L
Haydon
Rex C
RC
Deyrup
Andrea T
AT
Montag
Anthony G
AG
Huo
Dezheng
D
Heck
Robert
R
Heizmann
Claus W
CW
Peabody
Terrance D
TD
Simon
Michael A
MA
He
Tong-Chuan
TC
D000293:Adolescent; D000328:Adult; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology*; D002448:Cell Adhesion; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000502:physiology; D002465:Cell Movement; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009362:Neoplasm Metastasis / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology*; D010641:Phenotype; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000096:biosynthesis* / Q000502:physiology; D016019:Survival Analysis; D014407:Tumor Cells, Cultured
D018797:Cell Cycle Proteins; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C055351:S100A6 protein, human