title: Molecular characterization of Clostridium tetani strains by pulsed-field gel electrophoresis and colony PCR.
abstract: Pulsed-field gel electrophoresis and PCR were applied for the first time to the molecular characterization of Clostridium tetani. Among five strains tested, one (CN1339) turned out to contain a mixture of two genetically different clones and two (D11 and G761) to contain bacteria differing by the presence or absence of the 74-kb plasmid harboring the tetX gene.
authors.affiliation: Research Department, Aventis Pasteur SA, av. Marcel Mérieux, Marcy L'Etoile 69280, France.
authors.forename: Lucile
authors.initials: L
authors.lastname: Plourde-Owobi
authors.forename: Delphine
authors.initials: D
authors.lastname: Seguin
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Baudin
authors.forename: Catherine
authors.initials: C
authors.lastname: Moste
authors.forename: Bachra
authors.initials: B
authors.lastname: Rokbi
mesh_terms: D015373:Bacterial Typing Techniques; D003017:Clostridium tetani / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013744:Tetanus Toxin / Q000235:genetics*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D013744:Tetanus Toxin