Identification of new CNS-resident macrophage subpopulation molecular markers for the discrimination with murine systemic macrophages.
A controversial issue in neurobiology concerns the respective functions of central nervous system (CNS)-resident macrophages and systemic infiltrating macrophages morphologically and phenotypically similar during most of CNS injury processes. In a previous work, we isolated sixteen mRNAs differentially expressed between two microglial EOC clones. By studying their pattern of expression, we found that three of them were not expressed in peripheral macrophages, even after stimulation with IFNgamma, TNFalpha or IL10. These three molecules are physiologically expressed by murine adult microglia and could be used to evaluate in vivo their discriminative potential toward CNS-infiltrating macrophages during inflammatory events.
Donnou
Sabrina
S
Laboratoire de Biologie Moléculaire, Immunologie et Thérapeutique des Cancers (UPRES EA 3140), CHU, bâtiment Monteclair, 4 rue Larrey, 49033 ANGERS cedex 01, France.
Fisson
Sylvain
S
Mahe
Dominique
D
Montoni
Alicia
A
Couez
Dominique
D
D000818:Animals; D015703:Antigens, CD / Q000145:classification / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D015152:Blotting, Northern / Q000379:methods; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology*; D018076:DNA, Complementary / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D016753:Interleukin-10 / Q000494:pharmacology; D008262:Macrophage Activation / Q000502:physiology*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D017628:Microglia / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
D015703:Antigens, CD; D015415:Biomarkers; D018076:DNA, Complementary; D005904:Glial Fibrillary Acidic Protein; D016898:Interferon-alpha; D012333:RNA, Messenger; D016753:Interleukin-10; D007371:Interferon-gamma