[Isolation culture and identification of human neural stem cells from human embryos].
OBJECTIVE
To obtain and culture the human neural stem cells from the aborted human embryos.


METHODS
The neural stem cells were isolated and purified by the specific proliferous culture system of neural stem cells, and the molecular maker and multi-potency of the obtained neural stem cells were identified.


RESULTS
Human neural stem cells, which were isolated from 8 - 10 month old aborted human embryos, could express nestin ( a kind of specified antigen of the neural stem cell), and it could be differentiate into neurons and glials.


CONCLUSIONS
Neural stem cells can exist in human embryos, and it can be expanded in vitro.
Luo
Shu-Wei
SW
Human Reproductive Engineering Research Center, Xiangya School of Medicine , Central South University, Hunan Stem Cells Research Center, Changsha, China.
Xie
Chang-Qing
CQ
Lu
Guang-Xiu
GX
D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D009474:Neurons / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*