Monitoring DNA structures by dual fluorescence of pyrene derivatives.
We have developed a nucleotide modified by a pyrene derivative with dual fluorescence. The dual fluorescence of the fluorophore, which was incorporated into DNA, was effectively controlled at ambient temperature according to DNA structural status. Our nucleoside with dual fluorescence is effective as a conceptually new probe for monitoring DNA hybridization by the color change without multilabeling with fluorescent dyes.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan. okamoto@sbchem.kyoto-u.ac.jp
Tainaka
Kazuki
K
Nishiza
Ken-ichiro
K
Saito
Isao
I
D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D011721:Pyrenes / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature
D011721:Pyrenes; D004247:DNA