title: Human THROMBOSPONDIN-1 gene contains a natural antisense transcript, and characterization of its expression in human multiple tissues and cells.
abstract: Human THROMBOSPONDIN-1 play versatile roles in platelet aggregation, angiogenesis, and tumorigenesis, which forms a disulfide-linked homotrimeric complex. Here we reported that the genomic lotus of TSP1 also transcribes a natural antisense transcript (NAT) with an overlapping and reverse complement region against TSP1. The NAT of TSP1 was expressed in human testis, lung, thymus, colon, placenta, kidney and skeleton muscle, revealed by PCR amplification. It was also expressed in some tumor cell lines. The identification of NAT of TSP1 would be of significant importance to understand the functions of TSP1, and it would also suggest the potential attempt of using RNA interference for related tumor therapy, for instance, breast cancer.
authors.affiliation: Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, China.
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Ye
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Chen
authors.forename: Cong
authors.initials: C
authors.lastname: Chen
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Yu
authors.forename: Xin
authors.initials: X
authors.lastname: Gu
authors.forename: Chao-Qun
authors.initials: CQ
authors.lastname: Wu
authors.forename: Jin-Hu
authors.initials: JH
authors.lastname: Guo
authors.forename: Shou-Yuan
authors.initials: SY
authors.lastname: Zhao
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D016373:DNA, Antisense / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D013045:Species Specificity; D019700:Thrombospondin 1 / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D016373:DNA, Antisense; D019700:Thrombospondin 1