title: Proliferation and tube formation of periodontal endothelial cells.
abstract: Angiogenesis is indispensable to guide a regeneration of good periodontal tissue in the wound healing after periodontal surgery. Hepatocyte growth factor is well known for a strong angiogenic factor and it may play important roles in the periodontal tissue during periodontal wound healing. In exploring the promotion of angiogenesis in the periodontal ligament, proliferative and tubulogenic responses of endothelial cells to hepatocyte growth factor and to soluble factors secreted by fibroblasts were investigated. Pavement-shaped cells isolated from a human periodontal ligament were identified as the endothelial cell by their granular immunoreactivity for factor VIII. The proliferation of the endothelial cells was accelerated by the addition of hepatocyte growth factor or fibroblast-conditioned medium, and far more by adding both than either. The endothelial cells seeded on the agar containing both hepatocyte growth factor and fibroblast products formed a dense network in a shorter time than on the agar containing either. The endothelial cells in the dense network took a tube-like structure with lumen and were covered with laminin. These results suggest that hepatocyte growth factor administered into the regenerating periodontal tissue may promote, synergistically with local factors produced by the activated fibroblast, the proliferation and tubulogenesis of the remaining endothelial cells.
authors.affiliation: Department of Anatomy, The Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Etsuko
authors.initials: E
authors.lastname: Sato
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Hisahiro
authors.initials: H
authors.lastname: Kamoi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Tachibana
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Kyuichi
authors.initials: K
authors.lastname: Kamoi
mesh_terms: D000293:Adolescent; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology* / Q000648:ultrastructure; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000648:ultrastructure; D017228:Hepatocyte Growth Factor / Q000494:pharmacology / Q000502:physiology; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology*; D013268:Stimulation, Chemical
chemical_list: D017077:Culture Media, Conditioned; D017228:Hepatocyte Growth Factor; D003094:Collagen