title: An improved arbitrary primed PCR method for rapid characterization of transposon insertion sites.
abstract: Modifications were made to published arbitrary primed polymerase chain reaction (AP-PCR) procedures that resulted in increased specificity and sensitivity. Several arbitrary primer sequences were also evaluated, resulting in recommendations for primer design.
authors.affiliation: Philips Institute of Oral and Craniofacial Molecular Biology, Virginia Commonwealth University, Richmond, VA 23298-0566, USA.
authors.forename: Sankar
authors.initials: S
authors.lastname: Das
authors.forename: Jody C
authors.initials: JC
authors.lastname: Noe
authors.forename: Sehmi
authors.initials: S
authors.lastname: Paik
authors.forename: Todd
authors.initials: T
authors.lastname: Kitten
mesh_terms: D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013298:Streptococcus sanguis / Q000235:genetics
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements