title: Determination of 1,1,1,2,3,3,3-heptafluoropropane (HFP) in blood by headspace gas chromatography-mass spectrometry.
abstract: The inert and certified medical gas 1,1,1,2,3,3,3-heptafluoropropane (HFP) is commonly used as a propellant in medical sprays. HFP is also of interest as tracer gas for lung function measurements. In this work a sensitive method for quantitative determination of HFP in blood was developed using headspace gas chromatography-mass spectrometry with N2 as internal standard. The limits of detection and quantification were 0.005 and 0.016 mg/L, respectively. The method was applied to blood samples from newborn piglets that were ventilated with air containing 0.8% HFP (v/v) for 30 min. This was done to assess accumulation of HFP during measurement of functional residual lung capacity by wash-in and wash-out techniques. A saturation concentration in blood between 2.0 and 2.5 mg/L was attained after 3 min ventilation and decreased with a half-life below 1 min after termination of the HFP supply. No relevant accumulation of HFP was observed.
authors.affiliation: Institute of Legal Medicine, University Hospital Charité, Hittorftrasse 18, D-14195 Berlin, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Auwärter
authors.forename: Hans
authors.initials: H
authors.lastname: Proquitté
authors.forename: Gerd
authors.initials: G
authors.lastname: Schmalisch
authors.forename: Roland
authors.initials: R
authors.lastname: Wauer
authors.forename: Fritz
authors.initials: F
authors.lastname: Pragst
mesh_terms: D000336:Aerosols; D000818:Animals; D000831:Animals, Newborn; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry; D006845:Hydrocarbons, Fluorinated / Q000008:administration & dosage / Q000097:blood*; D012015:Reference Standards; D012121:Respiration, Artificial; D013552:Swine / Q000097:blood; D005466:Fluorocarbons; D011407:Propane / Q000031:analogs & derivatives
chemical_list: D000336:Aerosols; D006845:Hydrocarbons, Fluorinated; C414673:1,1,1,3,3,3-hexafluoropropane; D005466:Fluorocarbons; D011407:Propane