GABAergic system of the pineal organ of an elasmobranch (Scyliorhinus canicula): a developmental immunocytochemical study.
The present immunocytochemical study provides evidence of a previously unrecognized, rich, gamma-aminobutyric acid (GABA)-ergic innervation of the pineal organ in the dogfish (Scyliorhinus canicula). In this elasmobranch, the pineal primordium is initially detected at embryonic stage 24 and grows to form a long pineal tube by stage 28. Glutamic acid decarboxylase (GAD)-immunoreactive (-ir) fibers were first observed at stage 26, and by stage 28, thin GAD-ir fibers were detectable at the base of the pineal neuroepithelium. In pre-hatchling embryos, most fibers gave rise to GAD-ir boutons that were localized in the basal region of the neuroepithelium, although a smaller number of labeled terminals ascended to the pineal lumen. A few pale GAD-ir perikarya were observed within the pineal organ of stage 29 embryos, but GAD-ir perikarya were not observed at other developing stages or in adults. In contrast, GABA immunocytochemistry revealed the presence of GABAergic perikarya and fibers in the pineal organ of late stage embryos and adults. Although high densities of GABAergic cells were observed in the paracommissural pretectum, posterior tubercle, and tegmentum of dogfish embryos (regions previously demonstrated to contain pinealopetal cells), the presence of GABA-ir perikarya in the pineal organ strongly suggests that the rich GABAergic innervation of the elasmobranch pineal organ is intrinsic. This contrasts with the central origin of GABAergic fibers in the pineal gland of some mammals.
Carrera
Iván
I
Department of Cell Biology and Ecology, Faculty of Biology, University of Santiago de Compostela, 15706 Santiago de Compostela, Spain.
Sueiro
Catalina
C
Molist
Pilar
P
Holstein
Gay R
GR
Martinelli
Giorgio P
GP
Rodríguez-Moldes
Isabel
I
Anadón
Ramón
R
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004284:Dogfish / Q000502:physiology*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000201:enzymology; D010870:Pineal Gland / Q000196:embryology* / Q000294:innervation* / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D015415:Biomarkers; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase