Evaluation of ascorbic acid as suppressor of cyclophosphamide induced lipid peroxidation using common laboratory markers.
The present study deals with exploration of lipid peroxidation induction capacity of cyclophosphamide, an anticancer drug, and in vitro evaluation of ascorbic acid as a suppressor of cyclophosphamide induced lipid peroxidation. Goat liver homogenate has been used as the lipid source. This evaluation was done by measuring the malondialdehyde, 4-hydroxy-2-nonenal, reduced glutathione and nitric oxide content of the tissue as markers of lipid peroxidation. The study revealed that ascorbic acid could suppress the drug induced lipid peroxidation to a significant extent.
Ray
Supratim
S
Division of Medicinal & Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, India.
Sengupta
Chandana
C
Roy
Kunal
K
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D003520:Cyclophosphamide / Q000633:toxicity*; D004353:Drug Evaluation, Preclinical; D005978:Glutathione / Q000378:metabolism; D006041:Goats; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D013997:Time Factors
D000447:Aldehydes; D018906:Antineoplastic Agents, Alkylating; D000975:Antioxidants; D015415:Biomarkers; D009569:Nitric Oxide; D008315:Malondialdehyde; D003520:Cyclophosphamide; D005978:Glutathione; C027576:4-hydroxy-2-nonenal; D001205:Ascorbic Acid