Phenotypic convergence mediated by GGDEF-domain-containing proteins.
GGDEF domain-containing proteins have been implicated in bacterial signal transduction and synthesis of the second messenger molecule cyclic-di-GMP. A number of GGDEF proteins are involved in controlling the formation of extracellular matrices. AdrA (Salmonella enterica serovar Typhimurium) and HmsT (Yersinia pestis) contain GGDEF domains and are required for extracellular cellulose production and biofilm formation, respectively. Here we show that hmsT is able to restore cellulose synthesis to a Salmonella serovar Typhimurium adrA mutant and that adrA can replace hmsT in Y. pestis Hms-dependent biofilm formation. Like Y. pestis HmsT overproducers, Y. pestis cells carrying adrA under the control of an arabinose-inducible promoter produced substantial biofilms in the presence of arabinose. Finally, we demonstrate that HmsT is involved in the synthesis of cyclic di-GMP.
Simm
Roger
R
Department of Microbiology, Immunology, and Molecular Genetics, MS415 Medical Center, University of Kentucky, Lexington, KY 40536-0298, USA.
Fetherston
Jacqueline D
JD
Kader
Abdul
A
Römling
Ute
U
Perry
Robert D
RD
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism; D005816:Genetic Complementation Test; D010641:Phenotype; D010957:Plasmids; D017434:Protein Structure, Tertiary; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D015010:Yersinia pestis / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; C122178:HMST protein, Yersinia pestis; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP