Acquisition of lethal doses of Beauveria bassiana conidia by western flower thrips, Frankliniella occidentalis, exposed to foliar spray residues of formulated and unformulated conidia.
Secondary acquisition of Beauveria bassiana conidia was recorded on the whole bodies and selected body parts of second-instar nymphs and adult female western flower thrips exposed to foliar spray residues of three differently formulated conidial preparations, for 24 h. Conidia were formulated in emulsifiable oil or with clay (wettable powder), or were essentially unformulated conidia (technical grade powder suspended in water with a surfactant). Formulation had no significant effect on dose acquisition and no effect on virulence of acquired conidia. The mean nymphal LC50/LD50 was 116 conidia/mm2 and 52conidia/insect, respectively; the values for adults were 19 conidia/mm2 and 5conidia/insect. Greatest numbers of conidia were recorded on the legs and abdomens of nymphs and on the legs, wings, and thoraces of adults. As would be expected, numbers of conidia acquired increased with residue concentration (application rate). However, an inverse relationship was noted between acquisition rate (conidia acquired/total conidia applied) and residue concentration. The mechanism underlying this response was not determined. However, there was no indication that any body parts (e.g., tarsi) became saturated with spores, which suggests that either the thrips were repelled by the conidial residues or that as the concentrations of conidia on the substrate increased, conidia somehow became more difficult to acquire. Slopes of the LC probit regressions were lower than those of the LD regressions (mean 1.14 vs 1.78), suggesting that the low slopes often obtained in fungal pathogen assays could be partly an artifact of unequal rates of dose acquisition at low vs high application rates.
Ugine
Todd A
TA
Department of Entomology, Comstock Hall, Cornell University, Ithaca, NY 14853, USA. tau2@cornell.edu
Wraight
Stephen P
SP
Sanderson
John P
JP
D000336:Aerosols; D000818:Animals; D032661:Cordyceps / Q000472:pathogenicity*; D005247:Feeding Behavior; D005260:Female; D006430:Hemiptera / Q000254:growth & development / Q000382:microbiology*; D006790:Host-Parasite Interactions; D007928:Lethal Dose 50; D010572:Pest Control, Biological / Q000379:methods*; D035261:Plant Components, Aerial
D000336:Aerosols