Comparison of the use of aqueous and nonaqueous buffers in association with cyclodextrin for the chiral separation of 3,4-methylenedioxymethamphetamine and related compounds.
A comparison of the use of aqueous and nonaqueous buffers in association with beta-CD for the chiral separation of (R)- and (S)-3,4-methylenedioxymethamphetamine and related compounds is described. The (R)- and (S)-isomers of 3,4-methylenedioxymethamphetamine (MDMA) and its major metabolite 3,4-methylenedioxyamphetamine (MDA) were prepared. Under aqueous and nonaqueous buffer conditions and based on the CZE and MEKC modes, the order of migration of (R)-MDA, (S)-MDA, (R)-MDMA, and the (S)-MDMA enantioisomers were determined. Several electrophoretic parameters, including the concentration of beta-CD (aqueous, 25-60 mM; nonaqueous, 20-150 mM) used in the electrophoretic separation and the amount of organic solvents required for the separation, were optimized.
Huang
Yu-San
YS
Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan.
Tsai
Chung-Chen
CC
Liu
Ju-Tsung
JT
Lin
Cheng-Huang
CH
D015104:3,4-Methylenedioxyamphetamine / Q000031:analogs & derivatives / Q000302:isolation & purification; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000031:analogs & derivatives / Q000302:isolation & purification*; D012997:Solvents; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D002021:Buffers; D012997:Solvents; D047392:beta-Cyclodextrins; D015104:3,4-Methylenedioxyamphetamine; D018817:N-Methyl-3,4-methylenedioxyamphetamine