[Preparation and characterization of monoclonal antibodies against erythrocyte-binding antigen 175 of Plasmodium falciparum].
OBJECTIVE
To prepare and characterize the monoclonal antibodies (mAbs) against erythrocyte-binding antigen 175 of Plasmodium falciparum (EBA-175).


METHODS
BALB/ c mice were immunized with purified recombinant EBA-175 and mAbs against EBA-175 were prepared by means of hybridoma technique. Enzyme-linked immunosorbent assay (ELISA) and Western blotting were employed for characterization of the mAbs.


RESULTS
Six McAbs against EBA-175 antigen were obtained, 5 of which were identified as IgG1 and one as IgG2a. The titer of these aAbs was 1:12,800 to 1:25,600 in the ascites and 1:256 to 1:512 in supernatant, and ELISA demonstrated specific binding of the 4 mAbs (1F3, 2H5, 4A1 and 4H9)with Plasmodium falciparum. Three of these mAbs recognized the protein of EBA-175 as shown by Western blotting.


CONCLUSIONS
Six hybridoma cell lines secreting the mAbs against EBA175 with high specificity are successfully established.
Hao
Wen-bo
WB
School of Biotechnology, Southern Medical University, Guangzhou 510515, China. haowa@126.com
Hong
Yan-hua
YH
Sun
Xiao-min
XM
Ning
Yunshan
Y
Zhang
Dong-mei
DM
Pan
Wei-qing
WQ
Li
Ming
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C065668:erythrocyte-binding antigen 175, Plasmodium