Isolation of the salmonid rhamnose-binding lectin STL2 from spores of the microsporidian fish parasite Loma salmonae.
The microsporidian parasite, Loma salmonae, is the causative agent of gill disease in both wild and netpen-reared salmonids worldwide. In this paper we report the finding of a rhamnose-binding lectin from steelhead trout, Oncorhynchus mykiss, which was found bound in high concentration to the surface coat of L. salmonae spores. SDS-PAGE, immunoblot, N-terminal sequencing and mass spectrometric analyses were used to determine that the dominant 24 kDa protein lectin observed on SDS-PAGE analysis of intact spore extracts is the O. tshawytscha variant of the previously identified rhamnose-binding lectin STL2 from rainbow trout, O. mykiss. Although the physiological role of these lectins has not been clearly delineated, they have been implicated in a variety of functions, including inhibition of pathogenic bacteria by opsonization and macrophage-mediated tumour lysis.
Booy
A
A
University of Victoria - Genome British Columbia Proteomics Centre, Victoria, BC, Canada.
Haddow
J D
JD
Olafson
R W
RW
D000595:Amino Acid Sequence; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D029941:Fish Proteins / Q000235:genetics*; D015151:Immunoblotting; D037102:Lectins / Q000235:genetics*; D050262:Loma / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D012487:Salmonidae / Q000378:metabolism / Q000382:microbiology*; D020539:Sequence Analysis, Protein; D013172:Spores, Fungal / Q000378:metabolism*
D029941:Fish Proteins; D037102:Lectins; C442147:rhamnose-binding lectin, trout