Effect of hydrocarbons and other parameters on hydrocarbon-utilizing Pichia angusta MTCC-225.
Pichia angusta MTCC-225, a catalase-positive yeast that utilizes methanol and lighter hydrocarbons, is the subject of this investigation. An orthogonal experimental design L16 was used to investigate the effects of methanol, a gas mixture, zero air, temperature, agitation, and salts solution on hydrocarbon utilizing P. angusta. QUALITEK-4 Software was used for automatic design and analysis of the experimental results. Among the various parameters tested, agitation contributed the highest influence (56.5%). Zero air, methanol concentration, and gas mixture showed a moderate influence on the growth of P. angusta. Methanol concentration and gas mixture showed a 10.91 and 10.12% influence, respectively, on yeast growth. Zero air played an important role, with a 15.19% influence on the utilization of hydrocarbon.
Rao
R Sreenivas
RS
National Geophysical Research Institute, Uppal Road, Hyderabad-500007, India. srinu_ravella@yahoo.com
Rasheed
M A
MA
Kalpana
G
G
Patil
D J
DJ
Kumar
B
B
D000704:Analysis of Variance; D006838:Hydrocarbons / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism; D010843:Pichia / Q000378:metabolism* / Q000502:physiology*; D012492:Salts / Q000737:chemistry; D012984:Software; D013696:Temperature
D006838:Hydrocarbons; D012492:Salts; D000432:Methanol