title: GalNAc-GD1a is localized specifically in ventral spinal roots, but not in dorsal spinal roots.
abstract: We investigated the localization of GalNAc-GD1a biochemically in the human and bovine peripheral nervous system (PNS). The high-performance thin-layer chromatography (HPTLC)-overlay method with rabbit IgG polyclonal antibody against GalNAc-GD1a (anti-GalNAc-GD1a antibody) revealed expression of GalNAc-GD1a in the ventral spinal nerve roots (VRs) but not in the dorsal spinal nerve roots (DRs) of both species. The amount of GalNAc-GD1a in the human and bovine VRs was 2.22 +/- 0.35 microg/g wet tissue and 7.71 +/- 0.49 microg/g wet tissue, respectively. These results suggest that IgG anti-GalNAc-GD1a antibody may be involved in disturbance of peripheral motor nerves and in the pathogenesis of pure motor neuropathy.
authors.affiliation: Department of Neurology, Kohnodai Hospital, National Center of Neurology and Psychiatry, 1-7-1 Kohnodai, Ichikawa, Chiba 2720-8516, Japan.
authors.forename: Hiide
authors.initials: H
authors.lastname: Yoshino
authors.forename: Iku
authors.initials: I
authors.lastname: Utsunomiya
authors.forename: Kyoji
authors.initials: K
authors.lastname: Taguchi
authors.forename: Toshio
authors.initials: T
authors.lastname: Ariga
authors.forename: Takumi
authors.initials: T
authors.lastname: Nagaoka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aoyagi
authors.forename: Atsuko
authors.initials: A
authors.lastname: Asano
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Yamada
authors.forename: Tadashi
authors.initials: T
authors.lastname: Miyatake
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D013126:Spinal Nerve Roots / Q000276:immunology* / Q000378:metabolism*
chemical_list: D005732:Gangliosides; D007074:Immunoglobulin G; C055253:IV(4)-galactosyl-N-acetylganglioside GD1a