Rapid single nucleotide polymorphism mapping in C. elegans.
BACKGROUND
In C. elegans, single nucleotide polymorphisms (SNPs) can function as silent genetic markers, with applications ranging from classical two- and three-factor mapping to measuring recombination across whole chromosomes.


RESULTS
Here, we describe a set of 48 primer pairs that flank SNPs evenly spaced across the C. elegans genome and that work under identical PCR conditions. Each SNP in this set alters a DraI site, enabling rapid and parallel scoring. We describe a procedure using these reagents to quickly and reliably map mutations. We show that these techniques correctly map a known gene, dpy-5. We then use these techniques to map mutations in an uncharacterized strain, and show that its behavioral phenotype can be simultaneously mapped to three loci.


CONCLUSIONS
Together, the reagents and methods described represent a significant advance in the accurate, rapid and inexpensive mapping of genes in C. elegans.
Davis
M Wayne
MW
Department of Biology, University of Utah, Salt Lake City, Utah 84112-0840, USA. wdavis@biology.utah.edu
Hammarlund
Marc
M
Harrach
Tracey
T
Hullett
Patrick
P
Olsen
Shawn
S
Jorgensen
Erik M
EM
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D016678:Genome; D008297:Male; D008957:Models, Genetic; D009154:Mutation; D009710:Nucleotide Mapping; D010641:Phenotype; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011995:Recombination, Genetic
D017931:DNA Primers; D005819:Genetic Markers