title: Cloning and mapping of the porcine cytochrome-p450 2E1 gene and its association with skatole levels in the domestic pig.
abstract: The porcine cytochrome-p450 2E1 (CYP2E1) gene was isolated by screening a pig BAC library and partially sequenced. This sequence information was used to identify six single nucleotide polymorphisms (SNPs) within the CYP2E1 gene and its promoter. In addition, a microsatellite marker tightly linked to the CYP2E1 gene was subcloned from the BAC. One of these markers was used to map the CYP2E1 gene distal of SWC27 on SSC14, well outside reported quantitative trait loci on SSC14 for skatole, indole and taste test measures of boar taint. However, in a population of commercial pigs scored for backfat skatole levels, there was evidence of association between a SNP in the CYP2E1 promoter and skatole deposition, although there was no significant association between this SNP and skatole levels in the experimental cross.
authors.affiliation: Division of Genomics and Genetics, Roslin Institute, Roslin, Midlothian EH25 9PS, UK. tom.skinner@bbsrc.ac.uk
authors.forename: T M
authors.initials: TM
authors.lastname: Skinner
authors.forename: E
authors.initials: E
authors.lastname: Doran
authors.forename: J D
authors.initials: JD
authors.lastname: McGivan
authors.forename: C S
authors.initials: CS
authors.lastname: Haley
authors.forename: A L
authors.initials: AL
authors.lastname: Archibald
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D017931:DNA Primers; D005787:Gene Frequency; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D012862:Skatole / Q000378:metabolism*; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D012862:Skatole; D019392:Cytochrome P-450 CYP2E1