title: Characterization of a novel porin protein from Moraxella catarrhalis and identification of an immunodominant surface loop.
abstract: Moraxella catarrhalis is a gram-negative bacterium that is mainly responsible for respiratory tract infections. In this study we report a novel outer membrane protein (OMP), designated M35, with a molecular mass of 36.1 kDa. This protein was structurally homologous to classic gram-negative porins, such as OMP C from Escherichia coli and OMP K36 from Klebsiella pneumoniae, with a predicted structure of 8 surface loops and 16 antiparallel beta-sheets. The DNA sequences of the genes from 18 diverse clinical isolates showed that the gene was highly conserved (99.6 to 100% of nucleotides), with only one isolate (ID78LN266) having base variations that resulted in amino acid substitutions. Electrophoresis and analysis of recognition of the protein using mouse anti-M35 sera showed that M35 was expressed on the bacterial surface and constitutively expressed across M. catarrhalis isolates, with only ID78LN266 showing poor antibody recognition. Our results showed that the single amino acid mutation in loop 3 significantly affected antibody recognition, indicating that loop 3 appeared to contain an immunodominant B-cell epitope. The antibody specificity to loop 3 may be a potential mechanism for evasion of host immune responses targeted to M35, since loop 3 should theoretically orientate into the porin channel. Thus, M35 is a highly conserved, surface-expressed protein that is of significance for its potential functional role as an M. catarrhalis porin and is of interest as a vaccine candidate.
authors.affiliation: Division of Health, Design and Science, Gadi Research Centre, University of Canberra, Canberra, ACT 2601, Australia.
authors.forename: Donna M
authors.initials: DM
authors.lastname: Easton
authors.forename: Adam
authors.initials: A
authors.lastname: Smith
authors.forename: Sara Gomez
authors.initials: SG
authors.lastname: Gallego
authors.forename: A Ruth
authors.initials: AR
authors.lastname: Foxwell
authors.forename: Allan W
authors.initials: AW
authors.lastname: Cripps
authors.forename: Jennelle M
authors.initials: JM
authors.lastname: Kyd
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D005798:Genes, Bacterial; D001936:Moraxella catarrhalis / Q000737:chemistry*; D018272:Porins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018272:Porins