One-step screen-printed electrode modified in its bulk with HRP based on direct electron transfer for hydrogen peroxide detection in flow injection mode.
The preparation and performances of screen-printed carbon electrodes modified in their bulk with HRP (HRP-SPCE) is reported. The resulting modified HRP-SPCE was prepared in a one-step procedure, and then was optimised as an amperometric biosensor operating at [0-100] mV versus Ag/AgCl in flow injection mode for hydrogen peroxide. The amperometric response was due to direct electron transfer (DET) between HRP and SPCE surface. Factors such as chemical modification of the enzyme or the nature and rate of the binder were investigated regards to their influence on the sensitivity, linear range and operational stability. The best performing HRP-SPCE in terms of sensitivity and operational stability was obtained when graphite powder was modified with HRP previously oxidised by periodate ion (IO(4)(-)).
Ledru
Sophie
S
Groupe Méthodologie Electrochimique-LAIEM, UMR-CNRS 6006-FCMN, FR-CNRS 2465-Faculté des Sciences et des Techniques 2, Nantes, France.
Ruillé
Nadine
N
Boujtita
Mohammed
M
D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004579:Electron Transport; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011485:Protein Binding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D020099:Coated Materials, Biocompatible; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase