Bacteriochlorophyll-c homolog composition in green sulfur photosynthetic bacterium Chlorobium vibrioforme dependent on the concentration of sodium sulfide in liquid cultures.
Green sulfur photosynthetic bacteria Chlorobium (Chl.) vibrioforme (DSM 263 strain and NCIB 8327 substrain possessing BChl-c) and Chl. tepidum (ATCC 49652) were photoautotrophically grown in liquid cultures containing different concentrations of sodium sulfide (Na2S). BChl-c homologs possessing a methyl group at the 12-position tended to increase in cells of the two strains of Chl. vibrioforme cultured under high Na2S concentrations. In contrast, the Na2S concentration in liquid cultures did not affect the relative composition of BChl-c homologs in Chl. tepidum. 8-Propyl-12-methyl([P,M])-BChl-c homolog, which has been little observed in usual cultivations, could be isolated by reverse-phase high-performance liquid chromatography from the cells of Chl. vibrioforme grown under high Na2S contents. The [P,M]-BChl-c homolog has the R-configuration at the 3(1)-position, which was determined by 1H-NMR analyses.
Saga
Yoshitaka
Y
Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, 525-8577, Kusatsu, Shiga, Japan.
Osumi
Shigeaki
S
Higuchi
Hirohisa
H
Tamiaki
Hitoshi
H
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001429:Bacteriochlorophylls / Q000737:chemistry* / Q000378:metabolism; D041883:Chlorobium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013440:Sulfides / Q000494:pharmacology*
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D003470:Culture Media; D013440:Sulfides; C002084:bacteriochlorophyll c; C033479:sodium sulfide