Development of immunoassay for detection of meat and bone meal in animal feed.
An immunoassay system was developed for efficient detection of prohibited meat and bone meal (MBM) in animal feed. Monoclonal antibodies (MAbs) were raised against bovine smooth muscle autoclaved at 130 degrees C for 20 min. Among the 1,500 supernatants of hybridoma cells screened, MAbs 3E1, 1G3, and 3E10 were selected and characterized in this study. The first set of MAbs produced, 3E1 and 1G3, had stronger reactivity against MBM than against smooth muscle that was heat treated at 90 degrees C for 10 min. However, reactivity gradually increased against smooth muscle that was autoclaved at 130 degrees C for up to 1 h. The enzyme-linked immunosorbent assay for detection of MBM in animal feed was optimized with the MAb 3E10 because of its superior performance. MAb 3E10 diluted to 100-fold was used to differentiate bovine MBM from that of other species in ingredients used for commercial animal feeds and could detect down to 0.05% MBM mixed in animal feed.
Kim
Shin-Hee
SH
Department of Nutritional Sciences, College of Human Environmental Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA. kimshin@okstate.edu
Huang
Tung-Shi
TS
Seymour
Thomas A
TA
Wei
Cheng-I
CI
Kempf
Stephen C
SC
Bridgman
C Roger
CR
Momcilovic
Dragan
D
Clemens
Roger A
RA
An
Haejung
H
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D001688:Biological Products; D002417:Cattle; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis*; D006825:Hybridomas / Q000276:immunology; D008903:Minerals; D009130:Muscle, Smooth / Q000276:immunology*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000941:Antigens; D001688:Biological Products; D008903:Minerals; C027762:bone meal