Differentiation of a passive vaccine and the humoral immune response toward infection: analysis of phage displayed peptides.
Antibody-genes undergo molecular events that produce unique binding-sites that recognize specific epitopes, thus, leading to B-cell clonal variation. As a result, different binding-site structures (paratope internal images) are produced even when two distinct B-cells bind one and the same epitope. Paratope structural variation can be exploited to enable one to evaluate antibody-diversity in a single polyclonal serum sample. This is accomplished through the selection of antibody-specific peptides isolated from combinatorial phage displayed peptide libraries. As an example, we demonstrate the analysis of macaque sera containing passively administered antibodies, given as a therapeutic vaccine and antibodies actively produced by the virus-infected monkeys.
Siman-Tov
Dror D
DD
Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, P.O. Box 39040, Ramat-Aviv, Tel Aviv 69978, Israel. drorsimantov@yahoo.com
Navon-Perry
Leehee
L
Haigwood
Nancy L
NL
Gershoni
Jonathan M
JM
D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D001435:Bacteriophages / Q000276:immunology; D003429:Cross Reactions; D018604:Epitope Mapping; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics; D008253:Macaca mulatta; D019151:Peptide Library; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018449:SAIDS Vaccines / Q000276:immunology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control
D007074:Immunoglobulin G; D019151:Peptide Library; D018449:SAIDS Vaccines