Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen attack.
Cymbidium Mosaic Virus (CymMV) and Erwinia carotovora have been reported to cause severe damage to orchid plants. To enhance the resistance of orchids to both viral and bacterial phytopathogens, gene stacking was applied on Phalaenopsis orchid by double transformation. PLBs originally transformed with CymMV coat protein cDNA (CP) were then re-transformed with sweet pepper ferredoxin-like protein cDNA (Pflp) by Agrobacterium tumefaciens, to enable expression of dual (viral and bacterial) disease resistant traits. A non-antibiotic selection procedure in the second transformation minimized the potential rate of 'stacking' antibiotic genes in the orchid gene pool. Transgene integration in transgenic Phalaenopsis lines was confirmed by Southern blot analysis for both CP and pflp genes. Expression of transgenes was detected by northern blot analysis, and disease resistant assays revealed that transgenic lines exhibited enhanced resistance to CymMV and E. carotovora. This is the first report describing a transgenic Phalaenopsis orchid with dual resistance to phytopathogens.
Chan
Yuan-Li
YL
Graduate Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan.
Lin
Kuang-Hung
KH
Sanjaya
Liao
Li-Jen
LJ
Chen
Wen-Huei
WH
Chan
Ming-Tsair
MT
D002212:Capsicum / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D020868:Gene Silencing; D029595:Orchidaceae / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D016973:Pectobacterium carotovorum; D010935:Plant Diseases / Q000382:microbiology / Q000821:virology; D010942:Plant Viruses; D030821:Plants, Genetically Modified
D036022:Capsid Proteins