Novel probes for protein chip applications.
Protein microarrays (protein chips), due to their high throughput, have great potential to reduce the cycle time of drug discovery and to improve the efficiency of medical diagnostics. A great deal of effort has been made to facilitate the development of protein chips, such as the antibody microarrays. High-throughput fabrication of purified high-affinity probes is now one of the bottlenecks for protein microarrays. We discuss here three novel strategies to fabricate probes, namely, single chain antibodies displayed by phage, protein-oligonucleotide conjugates, and aptamers, which are promising in the protein chip technology.
Liao
Wei
W
Department of Chemical Biology, College of Chemistry, Peking University, Beijing 100871, China.
Guo
Su
S
Zhao
Xin Sheng
XS
D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques; D019295:Computational Biology; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D008027:Light; D008954:Models, Biological; D009841:Oligonucleotides / Q000737:chemistry*; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods*; D011487:Protein Conformation; D025941:Protein Interaction Mapping / Q000379:methods; D011506:Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods; D013499:Surface Properties
D000906:Antibodies; D007128:Immunoglobulin Fragments; D009841:Oligonucleotides; D019151:Peptide Library; D011506:Proteins; D020543:Proteome