Assessing phylogenetic relationships of Lycium samples using RAPD and entropy theory.
OBJECTIVE
To evaluate the phylogenetic relationships among related species of Lycium samples.


METHODS
Random amplified polymorphic DNA (RAPD) fingerprinting and lab-on-a-chip electrophoresis techniques were used to analyze the characteristics of Lycium species. Seven species and 3 varieties of Lycium were studied. Based on RAPD fingerprint data obtained from 11 primers, we proposed a new index, called dispersivity, using entropy theory and projection methods to depict the diversity of the DNA fingerprints.


RESULTS
Using the proposed dispersivity, primers were sorted and the dendrograms of the 7 species and 3 varieties of Lycium were constructed synthetically by merging primer information.


CONCLUSIONS
Phylogenetic relationships among Lycium samples were constructed synthetically based on RAPD fingerprint data generated from 11 primers.
Yin
Xiao-lin
XL
School of Mathematics and Statistics, Northeast Normal University, Changchun 130024, China. yinx1805@nenu.edu.cn
Fang
Kai-tai
KT
Liang
Yi-zeng
YZ
Wong
Ricky N S
RN
Ha
Amber W Y
AW
D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D019277:Entropy; D014644:Genetic Variation; D032305:Lycium / Q000145:classification / Q000235:genetics*; D046208:Microchip Analytical Procedures; D010802:Phylogeny; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
D017931:DNA Primers; D018744:DNA, Plant