title: Osteogenic differentiation of mesenchymal progenitor cells in computer designed fibrin-polymer-ceramic scaffolds manufactured by fused deposition modeling.
abstract: Biomimetic scaffolds offer great potentials in the development of bone analogs for tissue engineering. The studies presented in this paper focus specifically on the osteogenic potential of the novel PCL/CaP matrices and its degradation behavior. Biodegradable Polymer-ceramic Scaffolds were fabricated using the solid free form fabrication technology: Fused Deposition Modeling (FDM). The scaffold architecture was characterized by a honeycomb-like design and a complete interconnectivity of the pores. Human mesenchymal stem cells (MSCs) were seeded together with fibrin glue into PCL/CaP scaffolds and cultured in vitro for periods of up to eight weeks. Cellular adhesion, proliferation and osteogenic differentiation were assessed in these constructs using a range of histological and microscopic techniques. In additional experiments, degradation was assessed by measuring mass loss, diameter change, molecular weight change and by scanning electron micrographs. MSCs were able to adhere, migrate, and differentiate along the osteogenic lineage with in these scaffolds. The PCL/CaP scaffolds showed up to 27 fold increased degradation of compared to PCL scaffolds.
authors.affiliation: Division of Bioengineering, National University of Singapore, Singapore. surjts@nus.edu.sg
authors.forename: Jan-Thorsten
authors.initials: JT
authors.lastname: Schantz
authors.forename: Arthur
authors.initials: A
authors.lastname: Brandwood
authors.forename: Dietmar Werner
authors.initials: DW
authors.lastname: Hutmacher
authors.forename: Hwei Ling
authors.initials: HL
authors.lastname: Khor
authors.forename: Katharina
authors.initials: K
authors.lastname: Bittner
mesh_terms: D001673:Biodegradation, Environmental; D040761:Biomimetic Materials / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D003201:Computers; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005337:Fibrin / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D023822:Tissue Engineering / Q000379:methods; D014057:Tomography, X-Ray Computed
chemical_list: D002130:Calcium Phosphates; D005456:Fluorescent Dyes; D011091:Polyesters; D011108:Polymers; D015675:Osteocalcin; C016240:polycaprolactone; D005337:Fibrin; D016650:Fluorescein-5-isothiocyanate