Evidence for adjuvanticity of anthrax edema toxin.
Bacillus anthracis edema factor (EF) is an adenylate cyclase that increases intracellular cAMP concentrations. Since EF is present as a contaminant in the licensed protective antigen(PA)-based vaccines, we investigated its effect on anti-PA humoral immune response in BALB/c mice. We observed a significant increase of anti-PA IgG response in mice immunised with PA in association with EF as compared to PA alone. These results clearly show an adjuvant effect of EF, which is consistent with the data concerning the cellular effects of EF on antigen-presenting cells.
Quesnel-Hellmann
Anne
A
Unité d'Immunobiologie, Département de biologie des agents transmissibles, Centre de Recherches du service de Santé des Armées (CRSSA), BP87, F-38702 La Tronche cedex; France.
Cleret
Aurélie
A
Vidal
Dominique R
DR
Tournier
Jean-Nicolas
JN
D000262:Adenylyl Cyclases / Q000494:pharmacology*; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D000242:Cyclic AMP / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D007074:Immunoglobulin G; C030325:anthrax toxin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases