Runx1 function in hematopoiesis is required in cells that express Tek.
Runx1 expression marks the putative hemogenic endothelium between embryonic days (E) 8.5 to 11.5 of mouse gestation and is required for the formation of intra-aortic hematopoietic clusters, leading to the hypothesis that Runx1 is required for the transition from endothelial to hematopoietic cell. To address this hypothesis, we ablated the Runx1 gene by Cre-recombinase-mediated excision, with Cre expression under the control of the Tek promoter and enhancer. Most embryos died between E12.5 and E13.5 with a phenotype almost identical to Runx1 deficiency. We conclude that Runx1 function in establishing definitive hematopoiesis is required in a Tek+ cell.
Li
Zhe
Z
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA.
Chen
Michael J
MJ
Stacy
Terryl
T
Speck
Nancy A
NA
D000818:Animals; D050676:Core Binding Factor Alpha 2 Subunit / Q000502:physiology*; D004622:Embryo, Mammalian; D047108:Embryonic Development; D042783:Endothelial Cells / Q000166:cytology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D051379:Mice; D018345:Mice, Knockout; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D042787:Receptor, TIE-2; D013234:Stem Cells / Q000378:metabolism*
D050676:Core Binding Factor Alpha 2 Subunit; C493726:Runx1 protein, mouse; D020794:Receptor Protein-Tyrosine Kinases; D042787:Receptor, TIE-2; C079086:Tek protein, mouse