title: Fluviicola taffensis gen. nov., sp. nov., a novel freshwater bacterium of the family Cryomorphaceae in the phylum 'Bacteroidetes'.
abstract: A novel, strictly aerobic, Gram-negative, yellow-orange-pigmented, motile, catalase-positive, oxidase-negative bacterium, RW262(T), was isolated from water of the River Taff, Cardiff, UK, during January 2000. Phylogenetic analysis of the 16S rRNA gene indicated that strain RW262(T) was a member of the family Cryomorphaceae within the phylum 'Bacteroidetes'. The DNA G+C content of strain RW262(T) was 37.2 mol%. The predominant fatty acid was the branched-chain saturated fatty acid i15:0 (44.2%). On the basis of polyphasic analysis of phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, it is proposed that this freshwater bacterium represents a novel genus and species within the family Cryomorphaceae, Fluviicola taffensis gen. nov., sp. nov. The type strain is RW262(T) (=NCIMB 13979(T)=DSM 16823(T)).
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff CF10 3TL, UK.
authors.forename: Louise A
authors.initials: LA
authors.lastname: O'Sullivan
authors.affiliation: School of Earth, Ocean and Planetary Sciences, Cardiff University, Main Building, Park Place, Cardiff CF10 3YE, UK.
authors.forename: Joachim
authors.initials: J
authors.lastname: Rinna
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff CF10 3TL, UK.
authors.forename: Gavin
authors.initials: G
authors.lastname: Humphreys
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff CF10 3TL, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Weightman
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff CF10 3TL, UK.
authors.forename: John C
authors.initials: JC
authors.lastname: Fry
mesh_terms: D041963:Bacteroidetes / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S