A resistive-pulse sensor chip for multianalyte immunoassays.
MultiAnalyte immunoassays are often required to diagnose a pathologic condition. Here, we show how resistive-pulse sensing and multiple artificial pores can be integrated together on a single chip to detect different antigens rapidly and simultaneously. We use multiple pores on a single chip to detect the size change of latex colloids upon specific antigen-antibody binding on the colloid surface. As a proof-of-principle, we demonstrate our ability to detect simultaneously human G-CSF and GM-CSF antigens on a single chip. Our novel technique is a scalable technology that can lead to the sensing of at least N2 antigens simultaneously with an N N array of pores on a single chip.
Carbonaro
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A
Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA.
Sohn
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LL
D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004867:Equipment Design; D016179:Granulocyte Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D046208:Microchip Analytical Procedures / Q000379:methods; D008855:Microscopy, Electron, Scanning; D040081:Protein Array Analysis / Q000379:methods; D013223:Statistics as Topic
D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor