title: Gene expression profiling analysis of Mycobacterium tuberculosis genes in response to salicylate.
abstract: Salicylate stimulates the oxygen consumption and also induces multiple drug resistance in Mycobacterium tuberculosis. To gain insight into the mechanisms involved in the above observations, a microarray analysis of M. tuberculosis genes in response to salicylate was performed. Salicylate, besides highly inducing the 27 kD gene (Rv0560c) previously identified as highly salicylate-inducible, also caused increased transcription of a range of genes including an open reading frame (Rv0559c) that is located immediately downstream of the 27 kD gene, and some membrane/transmembrane proteins that may serve as potential efflux pumps or porins. Salicylate also caused a general shutdown of transcription and translation and energy production by down-regulating a range of genes involved in RNA and protein synthesis and ATP synthesis. The role of the salicylate-regulated genes in salicylate induced drug resistance and its unique effect on stimulating oxygen consumption in tubercle bacillus is discussed.
authors.affiliation: Department of Molecular Microbiology and Immunology Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD 21205, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Denkin
authors.forename: Sean
authors.initials: S
authors.lastname: Byrne
authors.forename: Charles
authors.initials: C
authors.lastname: Jie
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D016366:Open Reading Frames; D010101:Oxygen Consumption; D012329:RNA, Bacterial / Q000096:biosynthesis; D012980:Sodium Salicylate / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D012329:RNA, Bacterial; D000255:Adenosine Triphosphate; D012980:Sodium Salicylate