A microfluidic device using a green organic light emitting diode as an integrated excitation source.
A simply fabricated microfluidic device using a green organic light emitting diode (OLED) and thin film interference filter as integrated excitation source is presented and applied to fluorescence detection of proteins. A layer-by-layer compact system consisting of glass/PDMS microchip, pinhole, excitation filter and OLED is designed and equipped with a coaxial optical fiber and for fluorescence detection a 300 microm thick excitation filter is employed for eliminating nearly 80% of the unwanted light emitted by OLEDs which has overlaped with the fluorescence spectrum of the dyes. The distance between OLED illuminant and microchannels is limited to approximately 1 mm for sensitive detection. The achieved fluorescence signal of 300 microM Rhodamine 6G is about 13 times as high as that without the excitation filter and 3.5 times the result of a perpendicular detection structure. This system has been used for fluorescence detection of Rhodamine 6G, Alexa 532 and BSA conjugates in 4% linear polyacrymide (LPA) buffer (in 1 x TBE, pH 8.3) and 1.4 fmol and 35 fmol mass detection limits at 0.7 nl injection volume for Alexa and Rhodamine dye have been obtained, respectively.
Yao
Bo
B
Department of Chemistry, Tsinghua University, Beijing, 100084, China.
Luo
Guoan
G
Wang
Liduo
L
Gao
Yudi
Y
Lei
Gangtie
G
Ren
Kangning
K
Chen
Lingxin
L
Wang
Yiming
Y
Hu
Yan
Y
Qiu
Yong
Y
D000180:Acrylic Resins / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D019075:Electrophoresis, Capillary; D046209:Electrophoresis, Microchip / Q000379:methods; D005456:Fluorescent Dyes / Q000494:pharmacology; D005898:Glass; D008027:Light; D046208:Microchip Analytical Procedures / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence; D012235:Rhodamines / Q000494:pharmacology; D012709:Serum Albumin / Q000378:metabolism; D012834:Silver; D013997:Time Factors; D000099166:Polyacrylamides
D000180:Acrylic Resins; D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D012235:Rhodamines; D012709:Serum Albumin; D012834:Silver; D000099166:Polyacrylamides; C026188:rhodamine 6G