[Androgen receptor and SMRT].
OBJECTIVE
To explore the interaction between androgen receptor (AR) and silencing mediator for retinoid and thyroid hormone receptor (SMRT) and their interaction site. Methods We recombined and constructed AR, SMRT gene and gene fragments, in vitro translated 35S fusion proteins to investigate the relationship between AR and SMRT using transient transfection, mammalian two-hybrid test, GST pull-down assay, and indirect immunofluorescence staining. Results AR possessed an intrinsic transcriptional repression activity and AR interacted directly with SMRT. One interactive surface on AR was mapped to the ligand-binding domain (LBD), and the presence of DNA binding domain enhanced this interaction. The binding surface on SMRT was mapped to the carboxyl-terminal nuclear receptor interacting domain (ID), and mutation of the LXXXIXXXI/L corepressor motif within this domain interferred with the interaction.


CONCLUSIONS
LBD domain on the AR can interact with ID2 motif on the SMRT.
Liao
Guo-Qing
GQ
Department of Surgery, Xiangya Hospital, Central South University, Changsha 410008, China. liaoguoqing@medmail.com.cn
Tang
Hui-Huan
HH
Lü
Xin-Sheng
XS
D020816:Amino Acid Motifs / Q000502:physiology; D059002:Androgen Receptor Antagonists; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008297:Male; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D056971:Nuclear Receptor Co-Repressor 1; D056985:Nuclear Receptor Co-Repressor 2; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011944:Receptors, Androgen / Q000378:metabolism*; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism*
D059002:Androgen Receptor Antagonists; D004268:DNA-Binding Proteins; C539127:NCOR1 protein, human; C539130:NCOR2 protein, human; D009687:Nuclear Proteins; D056971:Nuclear Receptor Co-Repressor 1; D056985:Nuclear Receptor Co-Repressor 2; D011944:Receptors, Androgen; D012097:Repressor Proteins