title: Recombinant p-hydroxyphenylpyruvate dioxygenase of high activity.
abstract: Inhibitors of p-hydroxyphenylpyruvate dioxygenase (HPPD) are bleaching compounds impairing the formation of colored carotenoids. This activity makes them promising candidates for herbicides. Detailed studies on enzyme-inhibitor complexes or on the binding niche of the enzyme have still to be performed. Enzyme preparation from plants is time-consuming and the yield is poor. This paper describes in relevant detail the preparation of recombinant enzyme from Arabidopsis thaliana with good yield and high specific activity.
authors.affiliation: Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, D-78457 Konstanz, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Lederer
authors.forename: Peter
authors.initials: P
authors.lastname: Böger
mesh_terms: D015111:4-Hydroxyphenylpyruvate Dioxygenase / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D011994:Recombinant Proteins; D015111:4-Hydroxyphenylpyruvate Dioxygenase