title: Neutrophils from pregnant women produce thromboxane and tumor necrosis factor-alpha in response to linoleic acid and oxidative stress.
abstract: OBJECTIVE
  Preeclampsia is associated with oxidative stress, neutrophil activation, neutrophil infiltration into systemic vasculature, and elevated plasma levels of linoleic acid, the fatty acid precursor to arachidonic acid and its metabolite, thromboxane. In this study we evaluated whether linoleic acid under conditions of oxidative stress would stimulate neutrophil production of thromboxane and tumor necrosis factor-alpha.
  
  
  METHODS
  Neutrophils were isolated from 14 normal pregnant women. Western blot demonstrated cyclooxygenase-2 expression at 18 hours of incubation, so this incubation time was used for experiments. Neutrophils (2 x 10(6) cells/mL) were incubated in Dulbecco's modified Eagle's medium/F-12 with: (1) linoleic acid control; (2) an oxidizing solution enriched with linoleic acid; (3) oxidizing solution enriched with linoleic acid plus indomethacin; (4) oxidizing solution enriched with linoleic acid plus aspirin; (5) oxidizing solution enriched with linoleic acid plus NS-398, a specific inhibitor of cyclooxygenase-2; or (6) oxidizing solution enriched with linoleic acid plus pinane thromboxane, a thromboxane synthase inhibitor and receptor blocker.
  
  
  RESULTS
  Oxidizing solution enriched with linoleic acid significantly increased oxidative stress in neutrophils. Compared with linoleic acid, oxidizing solution enriched with linoleic acid significantly increased neutrophil production of thromboxane and tumor necrosis factor-alpha. Indomethacin and aspirin inhibited oxidizing solution enriched with linoleic acid stimulation of thromboxane, but NS-398 was equally effective implicating cyclooxygenase-2 in the thromboxane response. Indomethacin inhibited oxidizing solution enriched with linoleic acid stimulation of tumor necrosis factor-alpha, but so did pinane thromboxane implicating thromboxane in the tumor necrosis factor-alpha response.
  
  
  CONCLUSIONS
  These data demonstrate that exposure of neutrophils from normal pregnant women to conditions present in preeclamptic women results in neutrophil activation with release of thromboxane and tumor necrosis factor-alpha. Newly synthesized thromboxane is cyclooxygenase-2 dependent and plays a role in the tumor necrosis factor-alpha response. Our data suggest a mechanism for maternal vasoconstriction and vascular inflammation in preeclampsia because activated, thromboxane-secreting neutrophils migrate across endothelium into the microenvironment of the vasculature in which they could promote vasoconstriction, whereas release of tumor necrosis factor-alpha could cause vascular inflammation.
authors.affiliation: Departments of Obstetrics and Gynecology, Virginia Commonwealth University Medical Center, Richmond, VA, USA.
authors.forename: John E
authors.initials: JE
authors.lastname: Vaughan
authors.forename: Scott W
authors.initials: SW
authors.lastname: Walsh
mesh_terms: D015153:Blotting, Western; D005260:Female; D006801:Humans; D019787:Linoleic Acid / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000032:analysis; D009504:Neutrophils / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D011247:Pregnancy; D013931:Thromboxanes / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D008054:Lipid Peroxides; D013931:Thromboxanes; D014409:Tumor Necrosis Factor-alpha; D019787:Linoleic Acid