Measurement of immunoglobulins G, A, and M levels in B-lymphocytes culture.
Nephelometric immunoassays were developed for human IgG, IgA, and IgM quantitation in B-lymphocytes culture media. They allowed measurement of immunoglobulin (Ig) levels over a broad range of concentrations with good accuracy and precision. The kinetics of Ig production in B-lymphocyte cultures was followed and the mean amount of each Ig was determined in six different samples after three days of culture. The nephelometric immunoassays reported here could be used to study, in vitro, the influence of various molecules (inhibitory or amplifying effect) on B-lymphocytes' functional capacities.
Grandjenette
Cindy
C
GRIP, Immunology Laboratory, Faculty of Medecine, Vandoeuvre lés Nancy, France.
Kennel
Anne
A
Massin
Frédéric
F
Faure
Gilbert C
GC
Béné
Marie C
MC
Montagne
Paul
P
D001402:B-Lymphocytes / Q000737:chemistry*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003470:Culture Media / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D007223:Infant; D009391:Nephelometry and Turbidimetry / Q000379:methods; D014066:Palatine Tonsil / Q000166:cytology; D015203:Reproducibility of Results
D003470:Culture Media; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M