Crk-associated substrate lymphocyte type is required for lymphocyte trafficking and marginal zone B cell maintenance.
The lymphocyte-specific Cas family protein Cas-L (Crk-associated substrate lymphocyte type) has been implicated to function in lymphocyte movement, mediated mainly by integrin signaling. However, its physiological role is poorly understood. In this study we analyzed the function of Cas-L in lymphocytes using gene-targeted mice. The mutant mice showed a deficit of marginal zone B (MZB) cells and a decrease of cell number in secondary lymphoid organs. An insufficient chemotactic response and perturbed cell adhesion were observed in Cas-L-deficient lymphocytes, suggesting that the aberrant localization was responsible for the deficit of MZB cells. Moreover, we found that lymphocyte trafficking was altered in Cas-L-deficient mice, which gave a potential reason for contraction of secondary lymphoid tissues. Thus, Cas-L affects homeostasis of MZB cells and peripheral lymphoid organs, which is considered to be relevant to impaired lymphocyte migration and adhesion.
Seo
Sachiko
S
Department of Hematology and Oncology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
Asai
Takashi
T
Saito
Toshiki
T
Suzuki
Takahiro
T
Morishita
Yasuyuki
Y
Nakamoto
Tetsuya
T
Ichikawa
Motoshi
M
Yamamoto
Go
G
Kawazu
Masahito
M
Yamagata
Tetsuya
T
Sakai
Ryuichi
R
Mitani
Kinuko
K
Ogawa
Seishi
S
Kurokawa
Mineo
M
Chiba
Shigeru
S
Hirai
Hisamaru
H
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002634:Chemotaxis, Leukocyte; D006706:Homeostasis; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology; D008221:Lymphoid Tissue / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011506:Proteins / Q000502:physiology*
D048868:Adaptor Proteins, Signal Transducing; C505592:NEDD9 protein, mouse; D011506:Proteins