title: Development of a passive micromixer based on repeated fluid twisting and flattening, and its application to DNA purification.
abstract: We have developed a three-dimensional passive micromixer based on new mixing principles, fluid twisting and flattening. This micromixer is constructed by repeating two microchannel segments, a "main channel" and a "flattened channel", which are very different in size and are arranged perpendicularly. At the intersection of these segments the fluid inside the micromixer is twisted and then, in the flattened channel, the diffusion length is greatly reduced, achieving high mixing efficiency. Several types of micromixer were fabricated and the effect of microchannel geometry on mixing performance was evaluated. We also integrated this micromixer with a miniaturized DNA purification device, in which the concentration of the buffer solution could be rapidly changed, to perform DNA purification based on solid-phase extraction.
authors.affiliation: Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Nae Yoon
authors.initials: NY
authors.lastname: Lee
authors.forename: Masumi
authors.initials: M
authors.lastname: Yamada
authors.forename: Minoru
authors.initials: M
authors.lastname: Seki
mesh_terms: D005591:Chemical Fractionation / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D010812:Physical Stimulation / Q000295:instrumentation / Q000379:methods; D018511:Systems Integration
chemical_list: D004247:DNA