Characterization of cell lines developed from the glassy-winged sharpshooter, Homalodisca coagulata (Hemiptera: Cicadellidae).
Four continuous cell lines were established from the embryos of the glassy-winged sharpshooter, Homalodisca coagulata (Say), an economically important insect vector of bacterial pathogens of grape, almond, citrus, oleander, and other agricultural and ornamental plantings. The cell lines were designated GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH. The GWSS-Z10, GWSS-Z15, and GWSS-G3 lines were cultured in Ex-Cell 401 medium supplemented with 10% fetal bovine serum (FBS), whereas the GWSS-LH line was cultured in LH medium supplemented with 20% FBS. The cell lines were characterized in terms of their morphology, growth, protein composition, and polymerase chain reaction- amplification patterns of their chromosomal deoxyribonucleic acid. The population doubling times of GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH were 46.2, 90.9, 100.3, and 60.2 h, respectively. These lines should be useful for the study of insect-pathogenic viruses of leafhoppers, aphids, treehoppers, and other related insects as well as plant-pathogenic viruses that are transmitted by these insects.
Kamita
Shizuo G
SG
Department of Entomology, University of California-Davis, 1 Shields Avenue, Davis, CA 95616, USA.
Do
Zung N
ZN
Samra
Aman I
AI
Hagler
James R
JR
Hammock
Bruce D
BD
D000818:Animals; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002460:Cell Line / Q000166:cytology* / Q000378:metabolism; D049109:Cell Proliferation; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006430:Hemiptera / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D019476:Insect Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D019476:Insect Proteins; D004247:DNA