title: Solid phase microextraction for analysis of alkanes and aromatic hydrocarbons in human breath.
abstract: In this work, solid phase microextraction-gas chromatograph (SPME-GC) was applied to analyze alkanes and aromatic hydrocarbons in human breath, providing a potential non-invasive method to screen lung cancer. This method has been optimized and evaluated. It provided quantification limits ranging from 0.04 to 4.2 ng/mL, linear correlations ranging from 0.9845 to 0.9966 and R.S.D. values less than 9.8%. Total 30 breath samples, from 15 lung cancer patients and 15 healthy persons, were analyzed, and the alkanes and aromatic hydrocarbons were detected in 73.3% lung cancer patients and in 13.3% healthy persons by this method. Above all, It was demonstrated that this SPME-GC method provided a sensitive and non-invasive measure means to analyze alkanes and aromatic hydrocarbons in human breath, and brought forward a potential application for screening lung cancer.
authors.affiliation: Medical Electronic Engineering, Department of Electronics Communication Engineering, Shenzhen Polytechnic, Shenzhen 518055, China. vinyyu@hotmail.com
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000368:Aged; D000473:Alkanes / Q000032:analysis*; D001944:Breath Tests / Q000379:methods*; D002849:Chromatography, Gas / Q000379:methods*; D005260:Female; D006801:Humans; D006813:Humidity; D006841:Hydrocarbons, Aromatic / Q000032:analysis*; D008175:Lung Neoplasms / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D000473:Alkanes; D006841:Hydrocarbons, Aromatic