[Platelet-washing solution optimization].
Different washing and homogénéisation solutions are hereby analysed by comparing the evolution of functional indicators during the preservation of washed aphaeresis platelet concentrates: physiological pH 4.5 and 6 solutions, buffered physiological pH 6.8 glucose solution, and two physiological pH 7 citrate solutions with acetate. Prior acidification of platelet concentrates proved to be essential. Two washings with manual or automated technique, guarantee residual proteins at a level of less than 0.5 g. Solutions T-Sol Baxter or SSP Macopharma allow us to obtain a product that meet the PSL specifications. Routine since June 2004, washings are done with a physiological pH 6 solution, then homogeneised with T-Sol solution. Platelet recovery, swirling phenomenon, lack of agrgegates, pH maintenance, low increase in the platelet average volume and maintenance of intra-cell potassium level, suggest that platelet entirety is preserved beyond the product's expiration date. The platelet transfusion yield of these products is satisfactory.
Grossin
E
E
Etablissement de transfusion sanguine Ile-de-France, service préparation des produits sanguins labiles, site de Pontoise.
Chamfly
V
V
D000328:Adult; D000741:Anemia, Aplastic / Q000628:therapy; D001331:Automation / Q000295:instrumentation; D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects* / Q000528:radiation effects / Q000648:ultrastructure; D001798:Blood Proteins / Q000032:analysis; D002021:Buffers; D048429:Cell Size / Q000187:drug effects; D002498:Centrifugation; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017713:Platelet Transfusion / Q000331:legislation & jurisprudence / Q000592:standards; D010983:Plateletpheresis / Q000295:instrumentation / Q000379:methods*; D011188:Potassium / Q000097:blood; D012996:Solutions / Q000494:pharmacology*
D001798:Blood Proteins; D002021:Buffers; D012996:Solutions; D011188:Potassium