title: Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice.
abstract: The central hypothesis of this study was that matrix metalloproteinases (MMPs) would be enhanced following murine chlamydial infection and that their expression would vary in mouse strains that differ in their susceptibility to chronic chlamydia-induced disease. To address this hypothesis, female C3H/HeN and C57BL/6 mice were infected intravaginally with Chlamydia muridarum. Uterine and oviduct tissues were assessed for transcription of MMP genes and their tissue inhibitors. An increased activity of MMP genes relative to preinfection tissues was observed in the C3H/HeN mice when compared to C57BL/6 mice. Using gelatin zymography, we detected constitutive MMP-2 activity in both strains of mice but an increase in MMP-9. Casein zymography indicated the presence of two elastase-like activities consistent with MMP-12 and possibly MMP-7. Western blotting and antigen capture enzyme-linked immunoassay also confirmed an increase in MMP-9 but constitutive MMP-2 expression subsequent to the infection in both strains of mice. In C57BL/6 mice, MMP-9 was present in monomer and dimer form throughout the 56-day monitoring period. C3H/HeN mice produced dimeric MMP-9, but increases in the monomer form were also observed through day 14. Post-translational modification of MMP-9 between the two strains also differed. Immunohistochemistry revealed neutrophils as a prominent source for MMP-9 in both strains of mice. We conclude that differences in the relative expression and activity of MMPs, particularly MMP-9, occur in mice differing in their susceptibility to the development of chronic chlamydial disease. These differences may account for disparate outcomes with regard to chronic sequelae of the disease.
authors.affiliation: Department of Microbiology and Immunology, Chicago College of Osteopathic Medicine, Midwestern University, IL 60516, USA. kramse@midwestern.edu
authors.forename: K H
authors.initials: KH
authors.lastname: Ramsey
authors.forename: I M
authors.initials: IM
authors.lastname: Sigar
authors.forename: J H
authors.initials: JH
authors.lastname: Schripsema
authors.forename: N
authors.initials: N
authors.lastname: Shaba
authors.forename: K P
authors.initials: KP
authors.lastname: Cohoon
mesh_terms: D000818:Animals; D002690:Chlamydia Infections / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology; D023502:Chlamydia muridarum / Q000235:genetics; D019281:Dimerization; D005187:Fallopian Tubes / Q000378:metabolism; D005260:Female; D052776:Female Urogenital Diseases / Q000201:enzymology* / Q000382:microbiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D020782:Matrix Metalloproteinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000201:enzymology / Q000382:microbiology; D011499:Protein Processing, Post-Translational; D019714:Tissue Inhibitor of Metalloproteinases / Q000235:genetics / Q000378:metabolism; D014599:Uterus / Q000378:metabolism
chemical_list: D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases