Hsp27 expression coincides with epidermal stratification during human epidermal morphogenesis.
Heat shock protein 27 (Hsp27), apart from its protective function in response to stress, is implicated in the regulation of cell growth, differentiation and apoptosis. Data on the expression of Hsp27 in the developing human epidermis are sparse and partially conflicting. Thus, the purpose of the present study was to investigate Hsp27 expression during the morphogenesis of human epidermis. Skin biopsies and dispase-separated epidermal sheets obtained from 7 human embryos (7 and 8 weeks estimated gestational age, EGA), from 79 human fetuses (9-23 weeks EGA) and from 10 healthy adult volunteers were investigated by immunohistochemistry and Western blotting, respectively. The earliest detection of Hsp27 expression was found by immunohistochemistry at the 12th week EGA (basal and intermediate layer) and by Western blotting at the 9th week EGA. From the 16th to the 23rd week EGA immunoreactivity was not detectable in the basal layer, whereas in the overlying layers it revealed a differentiation-related pattern. The simultaneous onset of epidermal stratification and Hsp27 expression (9th week EGA) and the alterations of the latter in the subsequent stages of development, suggest that this stress protein may be involved in the molecular events underlying human epidermal morphogenesis.
Monastirli
Alexandra
A
Department of Dermatology, University of Patras, and Department of Obstetrics and Gynecology, Iasso General Hospital, Athens, Greece. almonast@med.upatras.gr
Vourekas
Anastassios
A
Badavanis
George
G
Pasmatzi
Efi
E
Sagriotis
Alexandros
A
Drainas
Denis
D
Pavlidou
Despina
D
Georgiou
Sophia
S
Sakkis
Theofilos
T
Mantagos
Stefanos
S
Kourounis
George
G
Varakis
John
J
Stamatiou
George
G
Tsambaos
Dionysios
D
D000328:Adult; D015153:Blotting, Western; D004817:Epidermis / Q000196:embryology* / Q000378:metabolism*; D005865:Gestational Age; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D009024:Morphogenesis
D006360:Heat-Shock Proteins