Cardioprotective effect of perfluorochemical emulsion for cardiac preservation after six-hour cold storage.
Perfluoro-octyl bromide (PFOB) emulsion is capable of transferring oxygen to tissues even at 4 degrees C, suggesting an application in myocardial preservation. We evaluated the cardioprotective effect of PFOB emulsion added to the storage solution. Guinea pig hearts were isolated (n = 24) and perfused with Krebs-Henseleit solution (KHS) and then cooled and perfused with St. Thomas Hospital II solution (STS). The hearts were stored in three different solutions: STS (STS group), oxygenated (O2) STS (O2 STS group), and O2 STS with PFOB emulsion (30%) (O2 STS + PFOB group) for 6 hours at 4 degrees C. After storage, rewarming was performed, followed by reperfusion with KHS. Left ventricular developed pressure of O2 STS + PFOB group was significantly higher than that of the other groups during reperfusion (p < 0.01). There was no difference among the three groups in O2 extraction and cardiac efficiency; however, cardiac oxygen consumption in the O2 STS + PFOB group significantly improved during reperfusion. In the O2 STS + PFOB group, creatinine kinase, lactate dehydrogenase, and myocardial water content were significantly decreased (p < 0.01). This study suggests that PFOB emulsion is beneficial for the cardioprotection of donor hearts, allowing a prolonged cardiac storage time.
Isaka
Mitsuhiro
M
Department of Cardiovascular Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
Imamura
Michiaki
M
Sakuma
Ichiro
I
Shiiya
Norihiko
N
Fukushima
Shoji
S
Nakai
Kunihiko
K
Kitabatake
Akira
A
Yasuda
Keishu
K
D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003402:Creatine Kinase / Q000378:metabolism; D015925:Cryopreservation; D004562:Electrocardiography; D004655:Emulsions; D005069:Evaluation Studies as Topic; D005466:Fluorocarbons / Q000494:pharmacology* / Q000627:therapeutic use; D005947:Glucose; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D006339:Heart Rate / Q000187:drug effects; D006842:Hydrocarbons, Brominated; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000737:chemistry; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010101:Oxygen Consumption; D010477:Perfusion; D011897:Random Allocation; D015424:Reperfusion; D013997:Time Factors; D014325:Tromethamine; D014867:Water / Q000032:analysis
D002316:Cardiotonic Agents; D004655:Emulsions; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; C074097:Krebs-Henseleit solution; D019188:Organ Preservation Solutions; D014325:Tromethamine; D014867:Water; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D005947:Glucose; C003072:perflubron