title: A novel splice variant of human gene NPL, mainly expressed in human liver, kidney and peripheral blood leukocyte.
abstract: From the human fetal brain cDNA library constructed by our lab, a novel variant cDNA of a human gene was successfully cloned and identified. Because the gene has been named N-acetylneuraminate pyruvate lyase (NPL), accordingly we term our splice variant NPL_v2. The cDNA of NPL_v2 has a full-length open reading frame (ORF) from the nucleotide position 320 to 1225 that encodes a protein comprising 301 amino acids. SMART analysis showed that our hypothetical protein has one dihydrodipicolinate synthase (DHDPS) domain. Phosphorylation analysis of the deduced protein show that there are five phosphorylation sites including three "serine" and two "threonine" at the region that are not found in other splice variant. RT-PCR experiment revealed that our splice variant of the gene is mainly expressed in human placenta, liver, kidney, pancreas, spleen, thymus, ovary, small intestine and peripheral blood leukocyte.
authors.forename: Maoqing
authors.initials: M
authors.lastname: Wu
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gu
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Xianqiong
authors.initials: X
authors.lastname: Zou
authors.forename: Huarui
authors.initials: H
authors.lastname: Zheng
authors.forename: Zhe
authors.initials: Z
authors.lastname: Jin
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D007652:Oxo-Acid-Lyases / Q000096:biosynthesis* / Q000235:genetics*; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000737:chemistry; D013552:Swine; D013912:Threonine / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D013912:Threonine; D012694:Serine; D007652:Oxo-Acid-Lyases; C018728:N-acetylneuraminate lyase