Binding of mycoplasmas to solid phase adsorbents.
The capture of mycoplasmas (M. hominis, M. buccale, M. fermentans, M. bovis, M. synoviae, M. gallisepticum and M. arthritidis) based on lipid structures and adhesion molecules present in the mycoplasmal membrane was tested using different chromatographic resins (ActiClean Etox, ClarEtox, Heparin-Actigel, Sulfated Hiflow and SulfEtox). All of the resins efficiently reduced mycoplasma concentrations in Phosphate Buffered Saline (PBS) and in Fetal Bovine Serum (FBS) by 3-8 logs in a few minutes. This technology could be used for removing mycoplasmas from tissue culture components such as serum, and for concentrating mycoplasmas in vaccine production.
Szathmáry
Susan
S
Clarigen Inc, Carlsbad, CA, USA.
Rajapakse
Nandani
N
Székely
Ibolya
I
Pitlik
E
E
Bíró
Judit
J
Erdei
Noémi
N
Stipkovits
L
L
D000327:Adsorption; D001422:Bacterial Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D002845:Chromatography; D009174:Mycoplasma / Q000302:isolation & purification* / Q000502:physiology*; D012117:Resins, Synthetic
D015815:Cell Adhesion Molecules; D012117:Resins, Synthetic