A comparison of Verotoxin B-subunit (Stx1B) and CD77 antibody to define germinal centre populations.
We have directly compared the use of a CD77 antibody with the binding subunit of Shiga-like toxin 1, Verotoxin 1, and (Stx1B) for delineation on human tonsil cells. We determined that the Stx1B produced a greater intensity of staining than the CD77 antibody, allowing three sub-populations of germinal centre cells to be seen. The populations express high, medium, and low levels of globotriaosylceramide as determined by the binding of the Stx1B reagent. The strong staining patterns of Stx1B suggest that it may be useful in defining germinal center B cell populations.
Bailey
S
S
Department of Immunology, Allergy and Arthritis, Flinders Medical Centre and Flinders University, Adelaide, SA, Australia.
Mardell
C
C
Wheatland
L
L
Zola
H
H
Macardle
P J
PJ
D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000379:methods; D018858:Germinal Center / Q000166:cytology / Q000378:metabolism; D006801:Humans; D014066:Palatine Tonsil / Q000276:immunology; D022603:Shiga Toxins / Q000276:immunology* / Q000378:metabolism; D014281:Trihexosylceramides / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D022603:Shiga Toxins; D014281:Trihexosylceramides; C077239:stxB toxin; C018549:globotriaosylceramide