Investigation of the expression of the EphB4 receptor tyrosine kinase in prostate carcinoma.
BACKGROUND
The EphB4 receptor tyrosine kinase has been reported as increased in tumours originating from several different tissues and its expression in a prostate cancer xenograft model has been reported.


METHODS
RT-PCR, western blotting and immunohistochemical techniques were used to examine EphB4 expression and protein levels in human prostate cancer cell lines LNCaP, DU145 and PC3. Immunohistochemistry was also used to examine localisation of EphB4 in tissue samples from 15 patients with prostate carcinomas.


RESULTS
All three prostate cancer cell lines expressed the EphB4 gene and protein. EphB4 immunoreactivity in vivo was significantly greater in human prostate cancers as compared with matched normal prostate epithelium and there appeared to be a trend towards increased expression with higher grade disease.


CONCLUSIONS
EphB4 is expressed in prostate cancer cell lines with increased expression in human prostate cancers when compared with matched normal tissue. EphB4 may therefore be a useful anti-prostate cancer target.
Lee
Yen-Ching
YC
Department of Haematology/Oncology, The Queen Elizabeth Hospital, Woodville, South Australia, 5011, Australia. ken.lee@student.adelaide.edu.au
Perren
Janeanne R
JR
Douglas
Evelyn L
EL
Raynor
Michael P
MP
Bartley
Maria A
MA
Bardy
Peter G
PG
Stephenson
Sally-Anne
SA
D000970:Antineoplastic Agents / Q000494:pharmacology; D015153:Blotting, Western; D002277:Carcinoma / Q000201:enzymology / Q000378:metabolism*; D045744:Cell Line, Tumor; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008856:Microscopy, Fluorescence; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000378:metabolism*; D036224:Receptor, EphB4 / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000970:Antineoplastic Agents; D036224:Receptor, EphB4