[Effects of recipes for replenishing qi and activating blood on senescence related gene expressions in the liver of aging rats].
OBJECTIVE
To observe the effects of recipes for replenishing qi and activating blood on p16, p21, proliferating cell nuclear antigen (PCNA), cyclin D1 and cyclin E gene expressions in the liver of aging rats.


METHODS
A recipe for replenishing qi and a recipe for activating blood were administered to aging rats respectively, and the effects of the above recipes on the expressions of senescence related genes (p16, p21, PCNA, cyclin D1 and cyclin E) were examined by RT-PCR and Western blotting methods.


RESULTS
The expressions of p16, p21 and cyclin D1 mRNAs and proteins in the liver of the untreated aging rats were up-regulated, while the expressions of PCNA and cyclin E mRNAs and proteins decreased. As compared with the untreated aging rats, both recipes could down-regulate the expressions of cyclin D1 mRNA and protein and up-regulate the expressions of cyclin E mRNA and protein, but had no obvious effects on the expressions of mRNAs and proteins of p16, p21 and PCNA.


CONCLUSIONS
Recipes for replenishing qi and activating blood can improve the liver cell proliferation of aging rats via down-regulating the expressions of cyclin D1 mRNA and protein and up-regulating the expressions of cyclin E mRNA and protein.
Li
Zhi-Ping
ZP
Department of Biochemistry, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. lizhiping1030@sina.com
Zhao
Wei-Kang
WK
Xu
Pin-Chu
PC
Chen
Fang-Min
FM
Jin
Guo-Qin
GQ
Yao
Ming-Zhong
MZ
D000375:Aging / Q000235:genetics*; D000818:Animals; D016922:Cellular Senescence; D019938:Cyclin D1 / Q000096:biosynthesis / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015870:Gene Expression; D008099:Liver / Q000378:metabolism*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis* / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D019941:Cyclin-Dependent Kinase Inhibitor p16; D004365:Drugs, Chinese Herbal; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D019938:Cyclin D1; D016283:Proto-Oncogene Proteins p21(ras)