Construction and direct immunization of eukaryotic expression plasmid encoding human soluble B lymphocyte stimulator.
B lymphocyte stimulator of the tumor-necrosis-factor family (BLyS) enhances B cell survival, a function indispensable to B cell maturation. This factor plays a crucial role in enhancing immune responses. Here, to study the primary immune effect of BLyS gene in animals, we constructed recombinant eukaryotic expression plasmid of the human soluble BLyS by using expression vector pcDNA3.1 (-). The recombinant plasmid pcDNA3.1BLyS was injected subcutaneously into BALb/C mice and this administration induced the sustained expression of human BLyS and specific IgG against the recombinant BLyS protein. This study shows that recombinant eukaryotic expression plasmid, pcDNA3.1BLyS, might be used in human gene therapy.
Chen
Guangyu
G
Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, Beijing, China.
Du
Hongwu
H
Peng
Shanyun
S
Xu
Donggang
D
Wang
Jiaxi
J
D000818:Animals; D000906:Antibodies; D053264:B-Cell Activating Factor; D001402:B-Lymphocytes / Q000737:chemistry* / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007107:Immune System / Q000378:metabolism* / Q000473:pathology*; D007136:Immunoglobulins / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D015233:Models, Statistical; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D000906:Antibodies; D053264:B-Cell Activating Factor; D016207:Cytokines; D007136:Immunoglobulins; D008565:Membrane Proteins; D018124:Receptors, Tumor Necrosis Factor; D011994:Recombinant Proteins; C505719:TNFSF13B protein, human; C505720:Tnfsf13b protein, mouse; D014409:Tumor Necrosis Factor-alpha