title: Thyroid hormone regulation of cell migration and oxidative metabolism in polymorphonuclear leukocytes: clinical evidence in thyroidectomized subjects on thyroxine replacement therapy.
abstract: Migration and superoxide anion (O2-) generation were studied in polymorphonuclear leukocytes (PMNs) from 14 athyreotic patients, previously treated by total thyroidectomy and radioiodine therapy for differentiated thyroid carcinoma, and from age- and sex-matched euthyroid healthy controls. Patients were studied twice: in hypothyroidism (visit 1) and after TSH-suppressive L-T4 replacement therapy (visit 2). Random migration and N-formyl-Met-Leu-Phe (fMLP) 0.1-microM induced chemotaxis were similar in cells from patients at both visit 1 and visit 2 and from healthy controls. On the contrary, resting O2- generation in cells from patients was significantly lower than control values, both at visit 1 and 2. At visit 1, fMLP 0.1 muM-induced O2- generation was significantly lower than control values, while phorbol-myristate acetate (PMA) 100-ng/ml induced O2- generation was similar in cells from patients and from controls. At visit 2 both responses increased, resulting in fMLP-induced O2- generation superimposable to control values and PMA-induced O2- generation significantly higher with respect to both visit 1 and cells from controls. In vitro exposure of PMNs from healthy subjects to L-T4 did not affect O2- generation in resting cells, and significantly increased that induced by fMLP or PMA only at high, supra-physiological concentrations. Neither TSH nor T3 had significant effects at any of the concentrations tested. The present results document the existence of a correlation between thyroid status and oxidative metabolism of human PMNs, which is however unlikely to depend upon a direct action of thyroid hormones on these cells.
authors.affiliation: Department of Clinical Medicine, Section of Experimental and Clinical Pharmacology, University of Insubria, Via Ottorino Rossi n. 9, 21100 Varese VA, Italy.
authors.forename: Franca
authors.initials: F
authors.lastname: Marino
authors.forename: Luigina
authors.initials: L
authors.lastname: Guasti
authors.forename: Marco
authors.initials: M
authors.lastname: Cosentino
authors.forename: Davide
authors.initials: D
authors.lastname: De Piazza
authors.forename: Cinzia
authors.initials: C
authors.lastname: Simoni
authors.forename: Eliana
authors.initials: E
authors.lastname: Piantanida
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Cimpanelli
authors.forename: Catherine
authors.initials: C
authors.lastname: Klersy
authors.forename: Luigi
authors.initials: L
authors.lastname: Bartalena
authors.forename: Achille
authors.initials: A
authors.lastname: Venco
authors.forename: Sergio
authors.initials: S
authors.lastname: Lecchini
mesh_terms: D000328:Adult; D000368:Aged; D002465:Cell Movement / Q000187:drug effects*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D005260:Female; D020249:Hormone Replacement Therapy; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D016897:Respiratory Burst / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism; D013963:Thyroid Hormones / Q000502:physiology*; D013965:Thyroidectomy; D013972:Thyrotropin / Q000494:pharmacology; D013974:Thyroxine / Q000096:biosynthesis / Q000627:therapeutic use*; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D013963:Thyroid Hormones; D014284:Triiodothyronine; D013481:Superoxides; D013972:Thyrotropin; D013974:Thyroxine