Changes in ascorbate peroxidase, catalase, guaiacol peroxidase and superoxide dismutase activities in common bean (Phaseolus vulgaris) nodules under salt stress.
To analyse nodular antioxidant enzyme expression in response to salt stress, Phaseolus vulgaris genotype BAT477 was inoculated with reference strain CIAT899, and treated with 50 mM NaCl. Plant growth, nodulation and nitrogen fixing activity were analysed. Results showed that: (1) all parameters, particularly in nodules, were affected by salt treatments, and (2) confirmed preferential growth allocation to roots. The ARA was significantly decreased by salt treatments. Protein dosage confirmed that nodules were more affected by salt treatment than were roots. We analysed superoxide dismutase, catalase, ascorbate peroxidase and peroxidase in nodules, roots and a free rhizobial strain. Our results indicated that SOD and CAT nodular isozymes had bacterial and root origins. The SOD expressed the same CuZn, Fe and Mn SOD isoforms in nodules and roots, whereas in free rhizobia we found only one Fe and Mn SOD. APX and POX nodule and root profiles had only root origins, as no rhizobial band was detected. Under salt stress, plant growth, nitrogen fixation and activities of antioxidant defense enzymes in nodules were affected. Thus, these enzymes appear to preserve symbiosis from stress turned out that NaCl salinity lead to a differential regulation of distinct SOD and POX isoenzyme. So their levels in nodules appeared to be consistent with a symbiotic nitrogen fixing efficiency hypothesis, and they seem to function as the molecular mechanisms underlying the nodule response to salinity.
Jebara
Salwa
S
Institut National de Recherche Scientifique et Technique, Laboratoire Interaction Légumineuses Microorganismes, BP 95, 2050 Hammam Lif, Tunisia.
Jebara
Moez
M
Limam
Férid
F
Aouani
Mohamed Elarbi
ME
D060387:Ascorbate Peroxidases; D002374:Catalase / Q000378:metabolism*; D004789:Enzyme Activation; D009195:Peroxidase / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D027805:Phaseolus / Q000201:enzymology*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology* / Q000382:microbiology; D012965:Sodium Chloride / Q000633:toxicity; D013482:Superoxide Dismutase / Q000378:metabolism*
D010940:Plant Proteins; D012965:Sodium Chloride; D010544:Peroxidases; C121048:guaiacol peroxidase; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase