title: Co-administration of porcine-specific CpG oligodeoxynucleotide enhances the immune responses to pseudorabies attenuated virus vaccine in newborn piglets in vivo.
abstract: Oligonucleotides containing CpG motifs (CpG ODN) are strong adjuvants for humoral and cellular immune responses in mice, but data on immune responses in piglets are scarce. In this report, porcine-specific CpG ODN were used as immunoadjuvants to enhance the immune responses of the newborn piglets to Pseudorabies attenuated virus (PRV) vaccine. The titres of specific antibodies and serum IgG1/IgG2 ratio to PRV vaccine, the proliferation of peripheral blood mononuclear cells (PBMCs), IL-4 and interferon-gamma(IFN-gamma) in piglets serum were examined to identify the immune response of the newborn piglets. The results showed that piglets immunized with PRV vaccine and CpG ODN presented high titers of PRV-specific antibodies and IgG2 isotype, a Th1-dominated (IFN-gamma) cytokine profile, together with inducing higher proliferation of PBMCs. All these data indicate that CpG ODN are potential effective adjuvants for the PRV vaccine in newborn piglets.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510640, China. lhzhang73212000@yahoo.com.cn
authors.forename: Zhang
authors.initials: Z
authors.lastname: Linghua
authors.forename: Guo
authors.initials: G
authors.lastname: Yong
authors.forename: Tian
authors.initials: T
authors.lastname: Xingshan
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fengzhen
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D018899:CpG Islands / Q000276:immunology*; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D009841:Oligonucleotides / Q000276:immunology*; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control*; D022421:Pseudorabies Vaccines / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control; D014613:Vaccines, Attenuated
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D009841:Oligonucleotides; D022421:Pseudorabies Vaccines; D014613:Vaccines, Attenuated; D015847:Interleukin-4; D007371:Interferon-gamma