title: [Effect of supernatants of bone marrow mononuclear cells on endothelial cell proliferation].
abstract: OBJECTIVE
  To define in vitro whether bone marrow mononuclear cells (BM-MNC) secrete vascular endothelial growth factor (VEGF) and to evaluate the effect of BM-MNC on endothelial cell proliferation.
  
  
  METHODS
  Rat BM-MNCs were isolated by centrifugation through a histopaque density gradient. BM-MNCs were cultured 4 weeks in vitro. Conditioned medium was assayed for VEGF and was added to cultured human umbilicus vein endothelial cells (HUVEC) to assess the effect of BM-MNC on endothelial cell (EC) proliferation.
  
  
  RESULTS
  Levels of VEGF in the conditioned medium increased compared with those in the control culture medium which was not exposed to BM-MNC. The conditioned medium enhanced, in a dose-related manner, HUVEC proliferation.
  
  
  CONCLUSIONS
  BM-MNCs is a valuable source of factors involved in angiogenesis. BM-MNCs secrete potent angiogenic cytokines such as VEGF and induce proliferation of endothelial cells.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China. liuwenliang1859@hotmail.com
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Liu
authors.forename: Bang-Liang
authors.initials: BL
authors.lastname: Ying
authors.forename: Feng-Lei
authors.initials: FL
authors.lastname: Yu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014471:Umbilical Veins / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D042461:Vascular Endothelial Growth Factor A