Immunogenicity of aerosol measles vaccine given as the primary measles immunization to nine-month-old Mexican children.
Aerosol measles vaccination has been found to be more immunogenic than subcutaneous administration as a booster in school aged children, and immunogenic in 12-month-old children as a primary dose. The objective of the study was to evaluate immunogenicity to aerosol measles vaccine in 9-month-old children.


METHODS
Nine-months-old infants received Edmonston-Zagreb measles vaccine by aerosol (10(3.58) CCID50/0.1 mL, estimated retained dose 10(2.81) CCID50 or subcutaneous route (10(4.28) CCID50/0.5 mL); cellular and humoral immunity and adverse events were assessed.


RESULTS
Measles-specific T cell proliferative responses developed in 42% of children given aerosolized vaccine compared with 67% of those who received subcutaneous vaccine (p = 0.01); the mean stimulation index (SI) was 4.4+/-0.7 versus 6.9+/-1, respectively, (p = 0.05). Seroconversion rates were 33 and 92% after aerosol or subcutaneous immunization (p < 0.001). Among infants who developed serologic responses, measles geometric mean titers (GMT; 95% CI) by neutralizing antibody assay were 215 mIU/mL (115-400) in aerosol vaccine recipients and 411 mIU/mL (345-490) in those given subcutaneous vaccine (p = 0.06).


CONCLUSIONS
The proportion of 9-month-old infants who developed cellular and/or humoral immunity to measles was lower in the aerosol group but measles antibody and T cell responses were comparable among those who developed measles immunity. Differences in response rates are attributable to the lower aerosol dose. Improving aerosol delivery or increasing the dose may enhance immunogenicity of primary aerosol measles vaccination in this age group.
Wong-Chew
Rosa María
RM
Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Dr. Balmis # 148, Colonia Doctores, 06726 Mexico City, México. rmwong@correo.unam.mx
Islas-Romero
Rocío
R
García-García
María de Lourdes
Mde L
Beeler
Judy A
JA
Audet
Susette
S
Santos-Preciado
Jose Ignacio
JI
Gans
Hayley
H
Lew-Yasukawa
Linda
L
Maldonado
Yvonne A
YA
Arvin
Ann M
AM
Valdespino-Gómez
José Luis
JL
D000336:Aerosols; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000917:Antibody Formation / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D007114:Immunization; D007223:Infant; D007279:Injections, Subcutaneous; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D008457:Measles / Q000276:immunology / Q000517:prevention & control*; D008458:Measles Vaccine / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology*; D008800:Mexico; D013601:T-Lymphocytes / Q000276:immunology; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D010948:Viral Plaque Assay
D000336:Aerosols; D000914:Antibodies, Viral; D008458:Measles Vaccine; D014613:Vaccines, Attenuated; D007371:Interferon-gamma