title: Oncolytic herpesvirus with secretable angiostatic proteins in the treatment of human lung cancer cells.
abstract: BACKGROUND
  The wild-type herpes simplex virus type 1 (HSV-1) has strong infectivity and cytolytic effect on almost all types of mammalian cells. Genetic engineering can now restrict this cytolysis to only malignant cells. G207 is an oncolytic HSV-1 vector developed based on this strategy.
  
  
  METHODS
  We used G207 as the backbone and integrated the exogenous endostatin-angiostatin fusion protein gene to generate a new vector, AE618.
  
  
  RESULTS
  Marked expressions of fusion protein in A549 and H460 lung cancer cells and culture medium were found 24 hours after treatment with AE618. In comparison with the G207 treatment group, the secreted protein from H460 cells treated with AE618 significantly inhibited the growth of human umbilical vein endothelial cells (HUVEC). AE618 also significantly inhibited the growth of xenografted tumors in vivo.
  
  
  CONCLUSIONS
  We propose that AE618 has the potential to be a novel anticancer agent with both oncolytic and anti-angiogenesis effects.
authors.affiliation: Department of Internal Medicine, Chang Gung Memorial Hospital, Chiayi, Taiwan.
authors.forename: Cheng-Ta
authors.initials: CT
authors.lastname: Yang
authors.forename: Yu-Chin
authors.initials: YC
authors.lastname: Lin
authors.forename: Chun-Liang
authors.initials: CL
authors.lastname: Lin
authors.forename: Jessica
authors.initials: J
authors.lastname: Lu
authors.forename: Xuexian
authors.initials: X
authors.lastname: Bu
authors.forename: Ying-Huang
authors.initials: YH
authors.lastname: Tsai
authors.forename: William W G
authors.initials: WW
authors.lastname: Jia
mesh_terms: D043168:Angiostatins / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000628:therapy* / Q000821:virology; D045744:Cell Line, Tumor; D043169:Endostatins / Q000096:biosynthesis / Q000235:genetics*; D042783:Endothelial Cells / Q000821:virology; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000235:genetics; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000628:therapy* / Q000821:virology; D051379:Mice; D016513:Mice, SCID; D021382:Protein Sorting Signals / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
chemical_list: D043169:Endostatins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D018664:Interleukin-12; D043168:Angiostatins