Disruption of Bardet-Biedl syndrome ciliary proteins perturbs planar cell polarity in vertebrates.
The evolutionarily conserved planar cell polarity (PCP) pathway (or noncanonical Wnt pathway) drives several important cellular processes, including epithelial cell polarization, cell migration and mitotic spindle orientation. In vertebrates, PCP genes have a vital role in polarized convergent extension movements during gastrulation and neurulation. Here we show that mice with mutations in genes involved in Bardet-Biedl syndrome (BBS), a disorder associated with ciliary dysfunction, share phenotypes with PCP mutants including open eyelids, neural tube defects and disrupted cochlear stereociliary bundles. Furthermore, we identify genetic interactions between BBS genes and a PCP gene in both mouse (Ltap, also called Vangl2) and zebrafish (vangl2). In zebrafish, the augmented phenotype results from enhanced defective convergent extension movements. We also show that Vangl2 localizes to the basal body and axoneme of ciliated cells, a pattern reminiscent of that of the BBS proteins. These data suggest that cilia are intrinsically involved in PCP processes.
Ross
Alison J
AJ
Molecular Medicine Unit, Institute of Child Health, University College London, WC1N 1EH, UK.
May-Simera
Helen
H
Eichers
Erica R
ER
Kai
Masatake
M
Hill
Josephine
J
Jagger
Daniel J
DJ
Leitch
Carmen C
CC
Chapple
J Paul
JP
Munro
Peter M
PM
Fisher
Shannon
S
Tan
Perciliz L
PL
Phillips
Helen M
HM
Leroux
Michel R
MR
Henderson
Deborah J
DJ
Murdoch
Jennifer N
JN
Copp
Andrew J
AJ
Eliot
Marie-Madeleine
MM
Lupski
James R
JR
Kemp
David T
DT
Dollfus
Hélène
H
Tada
Masazumi
M
Katsanis
Nicholas
N
Forge
Andrew
A
Beales
Philip L
PL
D000818:Animals; D020788:Bardet-Biedl Syndrome / Q000235:genetics / Q000473:pathology*; D016764:Cell Polarity / Q000235:genetics; D002923:Cilia / Q000737:chemistry; D003051:Cochlea / Q000473:pathology; D004847:Epithelial Cells / Q000737:chemistry; D005143:Eyelids / Q000503:physiopathology; D056385:Group II Chaperonins; D051379:Mice; D008817:Mice, Mutant Strains; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D018832:Molecular Chaperones / Q000235:genetics*; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D009436:Neural Tube Defects / Q000473:pathology; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism
C506281:BBS4 protein, mouse; C506280:Bbs1 protein, mouse; C431577:Ltap protein, mouse; D008869:Microtubule-Associated Proteins; C506279:Mkks protein, mouse; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; D056385:Group II Chaperonins