Lethal and severe coronary arteritis in DBA/2 mice induced by fungal pathogen, CAWS, Candida albicans water-soluble fraction.
CAWS is a microbial pathogen-associated molecular patterns (PAMPs) produced by Candida albicans. CAWS is a mannoprotein-beta-glucan complex and secreted into the culture supernatant. CAWS has various biological effects, causing acute shock and disrupting vascular permeability. Intraperitoneal administration of CAWS induces coronary arteritis in various strains of inbred mice. The CAWS-induced coronary arteritis is strain-dependent and most severe in DBA/2 mice with a significant number of these animals expiring with cardiomegaly during the observation period. In vivo and in vitro, splenocytes of DBA/2 mice produced various cytokines, such as IL-6, TNF-alpha, and IFN-gamma in response to CAWS. GM-CSF was also produced in response to CAWS. The production of cytokines was significantly enhanced in the presence of recombinant GM-CSF. In contrast, anti-GM-CSF significantly reduced the production of TNF-alpha and IFN-gamma. Augmented production of cytokines in response to CAWS would be a key to the severity of coronary arteritis.
Nagi-Miura
Noriko
N
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Harada
Toshie
T
Shinohara
Hiroyasu
H
Kurihara
Kiyoshi
K
Adachi
Yoshiyuki
Y
Ishida-Okawara
Akiko
A
Oharaseki
Toshiaki
T
Takahashi
Kei
K
Naoe
Shiro
S
Suzuki
Kazuo
K
Ohno
Naohito
N
D000818:Animals; D001167:Arteritis / Q000382:microbiology* / Q000401:mortality* / Q000473:pathology; D002176:Candida albicans / Q000472:pathogenicity*; D002458:Cell Fractionation; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000382:microbiology* / Q000473:pathology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000009:adverse effects / Q000502:physiology; D008297:Male; D008562:Membrane Glycoproteins / Q000009:adverse effects; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D012720:Severity of Illness Index; D012995:Solubility; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000473:pathology; D014867:Water / Q000008:administration & dosage*; D047071:beta-Glucans / Q000009:adverse effects
D008562:Membrane Glycoproteins; D047071:beta-Glucans; C025363:mannoproteins; D014867:Water; D016178:Granulocyte-Macrophage Colony-Stimulating Factor