Dissimilatory iron reduction and odor indicator abatement by biofilm communities in swine manure microcosms.
Animal waste odors arising from products of anaerobic microbial metabolism create community relations problems for livestock producers. We investigated a novel approach to swine waste odor reduction: the addition of FeCl(3), a commonly used coagulant in municipal wastewater treatment, to stimulate degradation of odorous compounds by dissimilatory iron-reducing bacteria (DIRB). Two hypotheses were tested: (i) FeCl(3) is an effective source of redox-active ferric iron (Fe(3+)) for dissimilatory reduction by bacteria indigenous to swine manure, and (ii) dissimilatory iron reduction results in significant degradation of odorous compounds within 7 days. Our results demonstrated that Fe(3+) from FeCl(3) was reduced biologically as well as chemically in laboratory microcosms prepared with prefiltered swine manure slurry and limestone gravel, which provided pH buffering and a substrate for microbial biofilm development. Addition of a 1-g liter(-1) equivalent concentration of Fe(3+) from FeCl(3), but not from presynthesized ferrihydrite, caused initial, rapid solids flocculation, chemical Fe(3+) reduction, and E(h) increase, followed by a 2-day lag period. Between 2 and 6 days of incubation, increases in Fe(2+) concentrations were accompanied by significant reductions in concentrations of volatile fatty acids used as odor indicators. Increases in Fe(2+) concentrations between 2 and 6 days did not occur in FeCl(3)-treated microcosms that were sterilized by gamma irradiation or amended with NaN(3), a respiratory inhibitor. DNA sequences obtained from rRNA gene amplicons of bacterial communities in FeCl(3)-treated microcosms were closely related to Desulfitobacterium spp., which are known representatives of DIRB. Use of iron respiration to abate wastewater odors warrants further investigation.
Castillo-Gonzalez
Hugo A
HA
Department of Crop and Soil Sciences, 116 ASI Building, The Pennsylvania State University, University Park, PA 16802, USA.
Bruns
Mary Ann
MA
D000822:Animal Husbandry; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D002712:Chlorides; D017753:Ecosystem; D005232:Fatty Acids, Volatile / Q000032:analysis; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008372:Manure / Q000382:microbiology*; D008969:Molecular Sequence Data; D009812:Odorants / Q000032:analysis*; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA; D013552:Swine
D002712:Chlorides; D005232:Fatty Acids, Volatile; D005290:Ferric Compounds; D008372:Manure; D007501:Iron; C024555:ferric chloride