A fast, simple, and reliable high-yielding method for DNA extraction from different plant species.
Genetic studies and pathogen detection in plants using molecular methods require the isolation of DNA from a large number of samples in a short time span. A rapid and versatile protocol for extracting high-quality DNA from different plant species is described. This method yields from 1 to 2 mg of DNA per gram of tissue. The absorbance ratios (A260/A280) obtained ranged from 1.6 to 2.0. A minimal presence of contaminating metabolites (as polymerase chain reaction [PCR] inhibitors) in samples and a considerable savings in reagents are characteristics of this protocol, as well as the low cost of the analysis per sample. The quality of the DNA was suitable for PCR amplification.
Tapia-Tussell
Raul
R
Laboratorio GeMBio, Centro de Investigación Científica de Yucatán, Calle 43, #130, Col. Chuburná de Hidalgo, Mérida 97200, Yucatán, México.
Quijano-Ramayo
Andres
A
Rojas-Herrera
Rafael
R
Larque-Saavedra
Alfonso
A
Perez-Brito
Daisy
D
D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D010944:Plants / Q000145:classification / Q000235:genetics*; D013997:Time Factors
D018744:DNA, Plant