title: Basic molecular biology.
abstract: Rapid advances in molecular biology over the past 20 years have and will continue to impact on the practice of medicine. Advances in molecular biology are having an immense impact in determining the underlying aetiology of lung disease and its treatment. In this review, basic molecular biology techniques will be discussed with examples of how these techniques are used in clinical practice.
authors.affiliation: Divisions of Neonatology and Pulmonary Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
authors.forename: Albert P
authors.initials: AP
authors.lastname: Senft
authors.forename: Ann Marie
authors.initials: AM
authors.lastname: LeVine
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008967:Molecular Biology; D016133:Polymerase Chain Reaction; D011486:Protein C / Q000378:metabolism; D012333:RNA, Messenger / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011486:Protein C; D012333:RNA, Messenger