[Establishment of 2-dE map of human low differentiation nasopharyngeal carcinoma cell line CNE-2 proteome].
OBJECTIVE
To establish a two-dimensional polyacrylamide gel electrophoresis (2-DE) map of human low differentiation nasopharyngeal carcinoma (NPC) cell line CNE-2 proteome.


METHODS
Immobilized pH gradient 2-DE was applied to separate the total proteins of CNE-2 cells, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) and database searching were used to indentify proteins.


RESULTS
A good 2-DE pattern with high resolution and reproducibility was obtained. Seventy-eight protein spots were incised from sliver staining gel and digested in gel by trypisin. Of them 77 maps of peptide mass fingerprints (PMF) were obtained and 48 proteins were identified.


CONCLUSIONS
A reference 2-DE map of CNE-2 proteome has been established and the data coupled with similar proteome analysis of other NPC cell lines will expand the human nasopharyngeal carcinoma proteome database.
Yang
Hai-Yan
HY
Key Laboratory of Cancer Proteonomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, China.
Xiao
Zhi-Qiang
ZQ
Li
Feng
F
D000595:Amino Acid Sequence; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry* / Q000473:pathology; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D010449:Peptide Mapping; D020543:Proteome / Q000302:isolation & purification
D009363:Neoplasm Proteins; D020543:Proteome