Characterisation of uterine sarcoma cell lines exhibiting MDR phenotype by vibrational spectroscopy.
Multidrug resistance (MDR) enables cancer cells to escape cytotoxic insults of anticancer drugs. Rapid identification of cells exhibiting the MDR phenotype is very important since it can lead to an effective and individual patient based treatment plan. We have investigated a combined vibrational spectroscopic approach, using both micro-Raman and FTIR techniques, in order to characterise a sensitive human uterine sarcoma cell line MES-SA and its multidrug-resistant derivative Garf. In this study, these two complementary methods have been evaluated via the use of principal components analysis (PCA), for discrimination of cells exhibiting the MDR phenotype. Our results indicate that, though they inherently have different sensitivities, both Raman and IR methods can provide a good differentiation of cell phenotypes.
Murali Krishna
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Unité MéDIAN, CNRS UMR 6142, UFR de Pharmacie, Université de Reims, 51, rue Cognacq Jay, 51906, REIMS, France.
Kegelaer
G
G
Adt
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I
Rubin
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S
Kartha
V B
VB
Manfait
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Sockalingum
G D
GD
D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D010641:Phenotype; D012509:Sarcoma / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods; D014594:Uterine Neoplasms / Q000737:chemistry*