title: Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen attack.
abstract: Cymbidium Mosaic Virus (CymMV) and Erwinia carotovora have been reported to cause severe damage to orchid plants. To enhance the resistance of orchids to both viral and bacterial phytopathogens, gene stacking was applied on Phalaenopsis orchid by double transformation. PLBs originally transformed with CymMV coat protein cDNA (CP) were then re-transformed with sweet pepper ferredoxin-like protein cDNA (Pflp) by Agrobacterium tumefaciens, to enable expression of dual (viral and bacterial) disease resistant traits. A non-antibiotic selection procedure in the second transformation minimized the potential rate of 'stacking' antibiotic genes in the orchid gene pool. Transgene integration in transgenic Phalaenopsis lines was confirmed by Southern blot analysis for both CP and pflp genes. Expression of transgenes was detected by northern blot analysis, and disease resistant assays revealed that transgenic lines exhibited enhanced resistance to CymMV and E. carotovora. This is the first report describing a transgenic Phalaenopsis orchid with dual resistance to phytopathogens.
authors.affiliation: Graduate Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Yuan-Li
authors.initials: YL
authors.lastname: Chan
authors.forename: Kuang-Hung
authors.initials: KH
authors.lastname: Lin
authors.lastname: Sanjaya
authors.forename: Li-Jen
authors.initials: LJ
authors.lastname: Liao
authors.forename: Wen-Huei
authors.initials: WH
authors.lastname: Chen
authors.forename: Ming-Tsair
authors.initials: MT
authors.lastname: Chan
mesh_terms: D002212:Capsicum / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D020868:Gene Silencing; D029595:Orchidaceae / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D016973:Pectobacterium carotovorum; D010935:Plant Diseases / Q000382:microbiology / Q000821:virology; D010942:Plant Viruses; D030821:Plants, Genetically Modified
chemical_list: D036022:Capsid Proteins