title: Effects of repeated Chlamydia pneumoniae inoculations on aortic lipid accumulation and inflammatory response in C57BL/6J mice.
abstract: Chlamydia pneumoniae is a common respiratory tract pathogen, and persistent infections have been associated with atherosclerosis. We studied the effects of repeated chlamydial inoculations on the inflammatory response and on aortic lipid accumulation in C57BL/6J mice. Mice fed a diet supplemented with 0.2% cholesterol were infected three or six times with C. pneumoniae every fourth week. Sera and lungs were analyzed for inflammatory responses, lung tissues were tested for the presence of C. pneumoniae DNA and RNA, and intimal lipid accumulation in the aortic sinus was quantified. High levels of chlamydial heat shock protein 60 (Hsp60) immunoglobulin G2c subclass antibodies were detected in all of the infected mice, and a positive and statistically significant correlation was found between these antibodies and autoantibodies against mouse Hsp60. Both Hsp60 antibody levels correlated with the severity of lung tissue inflammation. The cholesterol supplement in the diet had no effect on serum cholesterol levels. Significantly larger intimal lipid lesions were seen in the mouse group infected six times (6,542 mum(2)) than in the control group (1,376 mum(2); P = 0.034). In conclusion, repeated inoculations increased aortic sinus lipid accumulation in normocholesterolemic mice. The correlation between the antibodies to mouse and chlamydial Hsp60 proteins and their association with lung inflammation further support the theory of the development of an autoimmune response against heat shock proteins after repeated chlamydial infections.
authors.affiliation: National Public Health Institute, Oulu, Finland. liisa.tormakangas@ktl.fi
authors.forename: Liisa
authors.initials: L
authors.lastname: Törmäkangas
authors.forename: Leena
authors.initials: L
authors.lastname: Erkkilä
authors.forename: Taina
authors.initials: T
authors.lastname: Korhonen
authors.forename: Terttu
authors.initials: T
authors.lastname: Tiirola
authors.forename: Aini
authors.initials: A
authors.lastname: Bloigu
authors.forename: Pekka
authors.initials: P
authors.lastname: Saikku
authors.forename: Maija
authors.initials: M
authors.lastname: Leinonen
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001161:Arteriosclerosis / Q000209:etiology / Q000378:metabolism / Q000382:microbiology*; D018834:Chaperonin 60 / Q000276:immunology; D023521:Chlamydophila Infections / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D016993:Chlamydophila pneumoniae / Q000472:pathogenicity*; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D008168:Lung / Q000737:chemistry / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018410:Pneumonia, Bacterial / Q000378:metabolism / Q000382:microbiology*; D012329:RNA, Bacterial / Q000032:analysis; D012850:Sinus of Valsalva / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D014280:Triglycerides / Q000097:blood
chemical_list: D000907:Antibodies, Bacterial; D018834:Chaperonin 60; D002791:Cholesterol, Dietary; D004269:DNA, Bacterial; D008055:Lipids; D012329:RNA, Bacterial; D014280:Triglycerides; D002784:Cholesterol