title: The clpB gene of Bifidobacterium breve UCC 2003: transcriptional analysis and first insights into stress induction.
abstract: The so-called clp genes, which encode components of the Clp proteolytic complex, are widespread among bacteria. The Bifidobacterium breve UCC 2003 genome contains a clpB gene with significant homology to predicted clpB genes from other members of the Actinobacteridae group. The heat- and osmotic-inducibility of the B. breve UCC 2003 clpB homologue was verified by slot-blot analysis, while Northern blot and primer extension analyses showed that the clpB gene is transcribed as a monocistronic unit with a single promoter. The role of a hspR homologue, known to control the regulation of clpB and dnaK gene expression in other high G+C content bacteria was investigated by gel mobility shift assays. Moreover the predicted 3D structure of HspR provides further insight into the binding mode of this protein to the clpB promoter region, and highlights the key amino acid residues believed to be involved in the protein-DNA interaction.
authors.affiliation: Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland.
authors.forename: Marco
authors.initials: M
authors.lastname: Ventura
authors.affiliation: Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland.
authors.forename: John G
authors.initials: JG
authors.lastname: Kenny
authors.affiliation: Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland.
authors.forename: Ziding
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland.
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Fitzgerald
authors.affiliation: Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland.
authors.forename: Douwe
authors.initials: D
authors.lastname: van Sinderen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001644:Bifidobacterium / Q000235:genetics* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018869:Heat-Shock Response / Q000235:genetics / Q000502:physiology; D018832:Molecular Chaperones / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones