Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125.
A considerable fraction of life develops in the sea at temperatures lower than 15 degrees C. Little is known about the adaptive features selected under those conditions. We present the analysis of the genome sequence of the fast growing Antarctica bacterium Pseudoalteromonas haloplanktis TAC125. We find that it copes with the increased solubility of oxygen at low temperature by multiplying dioxygen scavenging while deleting whole pathways producing reactive oxygen species. Dioxygen-consuming lipid desaturases achieve both protection against oxygen and synthesis of lipids making the membrane fluid. A remarkable strategy for avoidance of reactive oxygen species generation is developed by P. haloplanktis, with elimination of the ubiquitous molybdopterin-dependent metabolism. The P. haloplanktis proteome reveals a concerted amino acid usage bias specific to psychrophiles, consistently appearing apt to accommodate asparagine, a residue prone to make proteins age. Adding to its originality, P. haloplanktis further differs from its marine counterparts with recruitment of a plasmid origin of replication for its second chromosome.
Médigue
Claudine
C
Genoscope, CNRS-UMR 8030, Atelier de Génomique Comparative, 91006 Evry Cedex, France.
Krin
Evelyne
E
Pascal
Géraldine
G
Barbe
Valérie
V
Bernsel
Andreas
A
Bertin
Philippe N
PN
Cheung
Frankie
F
Cruveiller
Stéphane
S
D'Amico
Salvino
S
Duilio
Angela
A
Fang
Gang
G
Feller
Georges
G
Ho
Christine
C
Mangenot
Sophie
S
Marino
Gennaro
G
Nilsson
Johan
J
Parrilli
Ermenegilda
E
Rocha
Eduardo P C
EP
Rouy
Zoé
Z
Sekowska
Agnieszka
A
Tutino
Maria Luisa
ML
Vallenet
David
D
von Heijne
Gunnar
G
Danchin
Antoine
A
D000222:Adaptation, Physiological / Q000235:genetics*; D003080:Cold Temperature; D016166:Free Radical Scavengers; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D020543:Proteome; D031161:Pseudoalteromonas / Q000235:genetics* / Q000502:physiology*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017382:Reactive Oxygen Species
D016166:Free Radical Scavengers; D020543:Proteome; D012329:RNA, Bacterial; D017382:Reactive Oxygen Species