title: Optimization of bacteriocin ST311LD production by Enterococcus faecium ST311LD, isolated from spoiled black olives.
abstract: Bacteriocin ST311LD is approximately 2.3 kDa in size. Low levels of bacteriocin activity were recorded in BHI and M17 broth (800 AU/ml) and in 10% (w/v) soy milk (3,200 AU/ml). No bacteriocin production was recorded in 10% (w/v) molasses, despite good growth. Optimal levels (12,800 AU/ml) were detected in MRS broth which had been supplemented with tryptone (20.0 g/l), saccharose (5.0 or 10.0 g/l) or vitamin C (1 ppm). Increased potassium levels did not result in higher levels of activity, and glycerol (1.0 g/l) inhibited the production of bacteriocin ST311LD.
authors.affiliation: Department of Microbiology, Stellenbosch University, 7600 Stellenbosch, South Africa.
authors.forename: Svetoslav D
authors.initials: SD
authors.lastname: Todorov
authors.forename: Leon M T
authors.initials: LM
authors.lastname: Dicks
mesh_terms: D001430:Bacteriocins / Q000096:biosynthesis*; D016984:Enterococcus faecium / Q000378:metabolism*; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D031658:Olea / Q000382:microbiology*
chemical_list: D001430:Bacteriocins