Identification of new antigens in visceral leishmaniasis by expression cloning and immunoblotting with sera of kala-azar patients from Bihar, India.
Sera of kala-azar patients from Bihar, India, were used to identify Leishmania donovani antigens encoded by a phage expression library. Ten antigens were identified, five of which have not been described as leishmania antigens before. The antigens specifically react with sera of leishmania-infected patients but not of toxoplasma- or plasmodium-infected patients.
Theinert
Stephan M
SM
Clinical Research Group, Department of Dermatology, Charité University Medicine Berlin, Humboldt University, Germany.
Basu
Rajatava
R
Forgber
Michael
M
Roy
Syamal
S
Sundar
Shyam
S
Walden
Peter
P
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology*; D000953:Antigens, Protozoan / Q000032:analysis* / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D006801:Humans; D015151:Immunoblotting; D007194:India; D007893:Leishmania donovani / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology*; D008969:Molecular Sequence Data
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan