[Microarray CGH: principle and use for constitutional disorders].
Chips technology has allowed to miniaturize process making possible to realize in one step and using the same device a lot of chemical reactions. The application of this technology to molecular cytogenetics resulted in the development of comparative genomic hybridization (CGH) on microarrays technique. Using this technique it is possible to detect very small genetic imbalances anywhere in the genome. Its usefulness has been well documented in cancer and more recently in constitutional disorders. In particular it has been used to detect interstitial and subtelomeric submicroscopic imbalances, to characterize their size at the molecular level or to define the breakpoints of translocation. The challenge today is to transfer this technology in laboratory medicine. Nevertheless this technology remains expensive and the existence of numerous sequence polymorphisms makes its interpretation difficult. Finally its is unlikely that it will make karyotyping obsolete as it does not allow to detect balanced rearrangements which after meiotic segregation might result in genome imbalance in the progeny.
Sanlaville
D
D
Service de cytogénétique, hôpital Necker-Enfants-malades, Assistance-publique-hôpitaux-de-Paris, 149, rue de Sèvres, 75015 Paris, France. damien.sanlaville@nck.ap-hop-paris.fr
Lapierre
J M
JM
Coquin
A
A
Turleau
C
C
Vermeesch
J
J
Colleaux
L
L
Borck
G
G
Vekemans
M
M
Aurias
A
A
Romana
S P
SP
D002648:Child; D003937:Diagnosis, Differential; D030342:Genetic Diseases, Inborn / Q000175:diagnosis*; D005820:Genetic Testing; D006801:Humans; D006824:Hybridization, Genetic; D007621:Karyotyping; D046228:Microarray Analysis / Q000379:methods*