title: High-performance liquid chromatography-tandem mass spectrometry validation of medroxyprogesterone acetate in products of pork origin and serum.
abstract: Different extraction and purification methods are described here to determine medroxyprogesterone acetate (MPA) in pork meat and serum. Spiked samples are investigated over the concentration range of MPA 0.5-20 ng/g. Pork meat tissues are subjected to extraction using organic solvent, and pork serum is simply diluted with acetate buffer. Clean-up is performed using solid-phase extraction on a C18 cartridge, and MPA is eluted with ethanol. Aliquots are injected into a high-performance liquid chromatography-mass spectrometry system. MPA content is determined on the basis of m/z 387-327 and 387-123 transitions.
authors.affiliation: Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Via Appia Nuova, 1411- Rome, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Giannetti
authors.forename: Daniela
authors.initials: D
authors.lastname: Barchi
authors.forename: Fulvia
authors.initials: F
authors.lastname: Fiorucci
authors.forename: Elisa
authors.initials: E
authors.lastname: Gennuso
authors.forename: Patrizia
authors.initials: P
authors.lastname: Sanna
authors.forename: Marco
authors.initials: M
authors.lastname: Pallagrosi
authors.forename: Bruno
authors.initials: B
authors.lastname: Neri
mesh_terms: D045930:Anabolic Agents / Q000032:analysis* / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat / Q000032:analysis*; D017258:Medroxyprogesterone Acetate / Q000032:analysis* / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D045930:Anabolic Agents; D017258:Medroxyprogesterone Acetate