The influence of cold shock proteins on transcription and translation studied in cell-free model systems.
Cold shock proteins (CSPs) form a family of highly conserved bacterial proteins capable of single-stranded nucleic acid binding. They are suggested to act as RNA chaperones during cold shock inhibiting the formation of RNA secondary structures, which are unfavourable for transcription and translation. To test this commonly accepted theory, isolated CSPs from a mesophilic, thermophilic and a hyperthermophilic bacterium (Bacillus subtilis, Bacillus caldolyticus and Thermotoga maritima) were studied in an Escherichia coli based cell free expression system on their capability of enhancing protein expression by reduction of mRNA secondary structures. The E. coli based expression of chloramphenicol acetyltransferase and of H-Ras served as model systems. We observed a concentration-dependent suppression of transcription and translation by the different CSPs which makes the considered addition of CSPs for enhancing the protein expression in in vitro translation systems obsolete. Protein expression was completely inhibited at CSP concentrations present under cold shock conditions. The CSP concentrations necessary for 50% inhibition were lowest (140 microm) for the protein of the hyperthermophilic and increased when the thermophilic (215 microm) or even the mesophilic protein (451 microm) was used. Isolated in vitro transcription under the influence of CSPs showed that the transcriptory effect is independent from the rest of the cell. It could be shown in a control experiment that the inhibition of protein expression can be removed by addition of hepta-2'-desoxy-thymidylate (dT7); a heptanucleotide that competitively binds to CSP. The data are in line with a hypothesis that CSPs act on bulk protein expression not as RNA chaperones but inhibit their transcription and translation by rather unspecific nucleic acid binding.
Hofweber
Roland
R
Institut für Biophysik und Physikalische Biochemie, Universität Regensburg, Germany.
Horn
Gudrun
G
Langmann
Thomas
T
Balbach
Jochen
J
Kremer
Werner
W
Schmitz
Gerd
G
Kalbitzer
Hans R
HR
D001407:Bacillus / Q000737:chemistry; D001426:Bacterial Proteins / Q000494:pharmacology*; D015500:Chloramphenicol O-Acetyltransferase; D003080:Cold Temperature; D005786:Gene Expression Regulation / Q000187:drug effects; D011905:Genes, ras; D006360:Heat-Shock Proteins / Q000494:pharmacology*; D018832:Molecular Chaperones / Q000494:pharmacology*; D009690:Nucleic Acid Conformation / Q000187:drug effects; D014176:Protein Biosynthesis / Q000187:drug effects*; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000494:pharmacology; D020124:Thermotoga maritima / Q000737:chemistry; D014158:Transcription, Genetic / Q000187:drug effects*
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D012329:RNA, Bacterial; D016601:RNA-Binding Proteins; D015500:Chloramphenicol O-Acetyltransferase