title: Comparison and evaluation of analysis procedures for the quantification of (2-methoxyethoxy)acetic acid in urine.
abstract: Several extraction and derivatization procedures were evaluated for the quantification of (2-methoxyethoxy)acetic acid (MEAA) in urine. MEAA is a metabolite and a biomarker for exposure to 2-(2-methoxyethoxy)ethanol, a glycol ether with widespread use in various industrial applications and the specific use as an anti-icing additive in the military jet fuel formulation JP-8. Quantification of glycol ether biomarkers is an active area of analytical research. Various sample preparation procedures were evaluated: liquid-liquid extraction (LLE) using ethyl acetate yielded the highest recovery, and solid-phase extraction (SPE) gave low recovery of MEAA. Two derivatization procedures were thoroughly investigated and validated, namely, silylation of MEAA with N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA), and esterification of MEAA using ethanol. Quantification was performed by gas chromatography (GC) with a mass spectrometer as detector and using a polydimethylsiloxane (HP-1) capillary column. Deuterated 2-butoxyacetic acid (d-BAA) was used as an internal standard. Recovery studies of spiked human urine demonstrated the accuracy and precision of both procedures. The limit of detection (LOD) and other figures of merit for both derivatization procedures will be discussed in detail. Applications of these analysis procedures are also discussed.
authors.affiliation: US Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Cincinnati, OH, 45226, USA. cbhymer@cdc.gov
authors.forename: Clayton
authors.initials: C
authors.lastname: B'Hymer
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Butler
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Cheever
mesh_terms: D000081:Acetamides; D000085:Acetates / Q000652:urine*; D015415:Biomarkers / Q000652:urine; D003903:Deuterium / Q000737:chemistry; D004951:Esterification; D000431:Ethanol / Q000031:analogs & derivatives / Q000737:chemistry; D005463:Fluoroacetates; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006016:Glycolates / Q000737:chemistry; D006801:Humans; D017646:Organosilicon Compounds / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014269:Trifluoroacetic Acid / Q000737:chemistry; D014556:Urine / Q000737:chemistry*
chemical_list: C487209:(2-methoxyethoxy)acetic acid; C403469:2-(2-methoxyethoxy)ethanol; D000081:Acetamides; D000085:Acetates; D015415:Biomarkers; D005463:Fluoroacetates; D006016:Glycolates; D017646:Organosilicon Compounds; C017095:n-butoxyacetic acid; D000431:Ethanol; C059151:N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide; D003903:Deuterium; D014269:Trifluoroacetic Acid