Changes in myofibrils and cytoskeleton of neonatal hamster myocardial cells in culture: an immunofluorescence study.
Myocardial cells in culture offer many possibilities for studying cellular and molecular biology of cardiac muscles. However, it is important to know how long these cells can be maintained in vitro without significant structural and biochemical changes. In this study, we have investigated the morphological changes of myofibril proteins and cytoskeletons by using immunofluorescent techniques in cultured neonatal hamster myocardial cells at different culture durations. Our results have demonstrated that these cultured cells still contain intact myofibrils and cytoskeletal proteins after 6 days in vitro incubation, however, the organization of some of these proteins is altered. The proteins most sensitive to these in vitro conditions are: myosin heavy chain, actin and desmin. The data indicate that the duration of the culture and the contractile activity of the myocardial cells in culture can influence organization of their contractile apparatus and cytoskeleton.
Zhang
C
C
Department of Biomedical Science, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431, USA.
Osinska
H E
HE
Lemanski
S L
SL
Huang
X P
XP
Lemanski
L F
LF
D000199:Actins / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival; D002478:Cells, Cultured; D064211:Connectin; D006224:Cricetinae; D003599:Cytoskeleton / Q000378:metabolism*; D003893:Desmin / Q000378:metabolism; D051180:Desmoplakins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D009124:Muscle Proteins / Q000378:metabolism; D009206:Myocardium / Q000166:cytology*; D009210:Myofibrils / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D016596:Vinculin / Q000378:metabolism
D000199:Actins; D064211:Connectin; D003893:Desmin; D051180:Desmoplakins; D009124:Muscle Proteins; D016596:Vinculin; D011494:Protein Kinases; D018995:Myosin Heavy Chains