Proopiomelanocortin gene expression and beta-endorphin localization in the pituitary, testis, and epididymis of stallion.
Proopiomelanocortin (POMC) is a precursor protein that contains the sequences of several bioactive peptides including adrenocorticotropin (ACTH), beta-endorphin (beta-EP), and melanocyte-stimulating-hormone (MSH). POMC is synthesized in the pituitary gland, brain, and many peripheral tissues. Immunoreactive POMC-derived peptides as well as POMC-like mRNA have been evidenced in several nonpituitary tissues, thus suggesting that POMC is actively synthesized by these tissues. The present study was aimed at evaluating if also in the case of stallion POMC-derived peptide, beta-EP, is produced locally in the testis, thus playing effects in a paracrine/autocrine fashion. To investigate this hypothesis the POMC gene expression was analyzed using 3' RACE-PCR and Northern Blot approaches in the testis and epididimys of stallion; moreover, immunocytochemical localization for beta-EP was also performed through confocal laser microscopy. The immunofluorescence results showed a positive beta-EP reaction not only in cellular nest of pituitary but also in the testis and genital tract of stallion, which function could be related with sperm mobility. Such role seem not to be no dependent on the peptide synthesized locally, because the molecular biology approach demonstrated the presence of POMC transcript in the pituitary only. In fact the Northern Blot analysis showed the presence of a single POMC transcript in the pituitary while no signal was detected in the testis and epididimys. The same results were obtained by applied 3' RACE-PCR analysis. In conclusion, opioid-derived peptide beta-EP is present in the genital tract of stallion, but is not locally produced as in other mammalian, and nonmammalian models; its possible biological function at testicular level could be linked to a long-loop feed-back mechanisms.
Soverchia
L
L
Dipartimento di Medicina Sperimentale e Sanità Pubblica, Università degli Studi di Camerino, via Scalzino 3, Camerino (MC), Italia.
Mosconi
G
G
Ruggeri
B
B
Ballarini
P
P
Catone
G
G
Degl'Innocenti
S
S
Nabissi
M
M
Polzonetti-Magni
A M
AM
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004822:Epididymis / Q000378:metabolism*; D006736:Horses / Q000378:metabolism*; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis* / Q000235:genetics*; D013737:Testis / Q000378:metabolism*; D001615:beta-Endorphin / Q000378:metabolism*
D001615:beta-Endorphin; D011333:Pro-Opiomelanocortin