title: High sequence diversity and evidence of balancing selection in the Pvmsp3alpha gene of Plasmodium vivax in the Venezuelan Amazon.
abstract: The genetic diversity of a defined Plasmodium vivax population from the Venezuelan Amazon was evaluated by direct sequencing of the gene encoding the P. vivax merozoite surface protein-3alpha, Pvmsp3alpha. Three allele sizes (1.9, 1.4 and 1.1kb) were amplified from 58 isolates with frequencies of 59.3%, 21.9% and 18.8%, respectively. 27 Pvmsp3alpha nucleotide sequences were determined, with nine distinct haplotypes observed. The genetic diversity (h) at this single locus was 0.774. The P. vivax population in this region exhibits significant diversity in contrast to the genetically restricted diversity of the sympatric P. falciparum population. Despite sharing vector and human hosts, different control strategies may be required for these two species in this region. Substitution patterns in the conserved C-terminus of Pvmsp3alpha showed a significant departure from neutrality, suggesting these polymorphisms are being maintained by frequency-dependent selection as the result of an effective immune response from the host. Our findings support the use of Pvmsp3alpha genotyping as a tool for monitoring interventions aimed at control of P. vivax.
authors.affiliation: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK. rosalynn.ord@uclh.nhs.uk
authors.forename: Rosalynn
authors.initials: R
authors.lastname: Ord
authors.forename: Spencer
authors.initials: S
authors.lastname: Polley
authors.forename: Adriana
authors.initials: A
authors.lastname: Tami
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Sutherland
mesh_terms: D000483:Alleles; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D001483:Base Sequence; D014644:Genetic Variation; D006801:Humans; D016780:Malaria, Vivax / Q000469:parasitology; D010802:Phylogeny; D010966:Plasmodium vivax / Q000235:genetics*; D017434:Protein Structure, Tertiary / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics*; D012641:Selection, Genetic; D014687:Venezuela
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C089133:merozoite surface protein 3, Plasmodium