Staphylococcus simiae sp. nov., isolated from South American squirrel monkeys.
Eight coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococcal strains were isolated from the gastrointestinal tracts of South American squirrel monkeys (Saimiri sciureus L.). These strains were differentiated from known staphylococcal species on the basis of 16S rRNA gene and hsp60 gene sequencing, and from the most closely related species by using DNA-DNA hybridization, ribotyping, whole-cell protein profiles and biotyping. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that these strains are members of the Staphylococcus aureus species group (99% similarity) but are biochemically similar to Staphylococcus piscifermentans, from which they can be phenotypically distinguished by resistance to polymyxin B, acid production from D-mannitol, the inability to hydrolyse aesculin and DNA and the absence of alpha-glucosidase. On the basis of these analyses, a novel species of the genus Staphylococcus is described, for which the name Staphylococcus simiae sp. nov. is proposed, with CCM 7213(T) (=LMG 22723(T)) as the type strain.
Pantůček
Roman
R
Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic.
Sedláček
Ivo
I
Czech Collection of Microorganisms, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
Petráš
Petr
P
Reference Laboratory for Staphylococci, National Institute of Public Health, Šrobárova 48, 100 42 Prague, Czech Republic.
Koukalová
Dagmar
D
Institute of Microbiology, Faculty of Medicine, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic.
Švec
Pavel
P
Czech Collection of Microorganisms, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
Štětina
Vlastimil
V
Czech Collection of Microorganisms, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
Vancanneyt
Marc
M
BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Chrastinová
Lenka
L
Zoo Olomouc, Darwinova 29, 772 00 Olomouc, Czech Republic.
Vokurková
Jitka
J
Zoo Olomouc, Darwinova 29, 772 00 Olomouc, Czech Republic.
Růžičková
Vladislava
V
Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic.
Doškař
Jiří
J
Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic.
Swings
Jean
J
BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Hájek
Václav
V
Institute of Microbiology, Faculty of Medicine, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic.
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D041981:Gastrointestinal Tract / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D012453:Saimiri / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013020:South America; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S