Down-regulation of SMT3A gene expression in association with DNA synthesis induction after X-ray irradiation in nevoid basal cell carcinoma syndrome (NBCCS) cells.
Fibroblast cells derived from nevoid basal carcinoma syndrome (NBCCS) patients show increased levels of DNA synthesis after X-ray irradiation. Genes, whose expression is modulated in association with the DNA synthesis induction, were searched by using PCR-based mRNA differential display analysis in one of the NBCCS cell lines, NBCCS1 cells. Decreased levels of SMT3A gene expression were found in X-ray-irradiated NBCCS1 cells. This decrease was also shown by RT-PCR analysis in another cell line, NBCCS3 cells. In addition to NBCCS cells, normal fibroblast cells showed the DNA synthesis induction after X-ray irradiation when they were treated with antisense oligonucleotides (AO) for SMT3A. However, treatment of normal fibroblasts with the random oligonucleotides (RO) resulted in decreased levels of DNA synthesis after X-ray irradiation. Thus, down-regulation of SMT3A gene expression may be involved in the DNA synthesis induction after X-ray irradiation in the NBCCS cells at least tested.
Sugaya
Shigeru
S
Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chiba 260-8670, Japan.
Nakanishi
Hiroshi
H
Tanzawa
Hideki
H
Sugita
Katsuo
K
Kita
Kazuko
K
Suzuki
Nobuo
N
D001478:Basal Cell Nevus Syndrome / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000528:radiation effects; D015536:Down-Regulation / Q000528:radiation effects*; D004996:Ethidium / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D006801:Humans; D007700:Kinetics; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism*; D014965:X-Rays
D004273:DNA, Neoplasm; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C492189:SUMO3 protein, human; D014452:Ubiquitins; D004996:Ethidium