title: Beta2-adrenoceptor agonists induce the mammalian clock gene, hPer1, mRNA in cultured human bronchial epithelium cells in vitro.
abstract: The mammalian Per1 gene is one of the most important components of circadian clock function of the suprachiasmatic nucleus and peripheral tissues. We examined whether the beta2-adrenoceptor agonists, procaterol and fenoterol, induce human Per1 mRNA expression in human bronchial epithelium. The in vitro stimulation of beta2-adrenoceptor agonists in BEAS-2B cells led to a remarkable increase in the level of hPer1 mRNA. Moreover, fenoterol or procaterol induced the phosphorylation of CREB in BEAS-2B cells as verified by immunoblot analysis. beta2-adrenoceptor agonists induced human Per1 mRNA expression by the signaling pathways of cAMP-CREB in BEAS-2B cells.
authors.affiliation: Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, Japan.
authors.forename: Miyako
authors.initials: M
authors.lastname: Takata
authors.forename: Naoto
authors.initials: N
authors.lastname: Burioka
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Ohdo
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Fukuoka
authors.forename: Masanori
authors.initials: M
authors.lastname: Miyata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Endo
authors.forename: Hisashi
authors.initials: H
authors.lastname: Suyama
authors.forename: Eiji
authors.initials: E
authors.lastname: Shimizu
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism; D018797:Cell Cycle Proteins; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005280:Fenoterol / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D009687:Nuclear Proteins / Q000096:biosynthesis*; D056950:Period Circadian Proteins; D017265:Procaterol / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C539022:PER1 protein, human; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D005280:Fenoterol; D017265:Procaterol