Evidence for inclusion of a segment of Escherichia coli genomic DNA in bovine tooth germ mRNA encoding salivary proline-rich protein P-B.
In the course of cloning of bovine cDNA for proline-rich protein (PRP) P-B from bovine tooth germ cDNA, we found that one clone with 662 bp contained a 5'-terminal 393 bp (1-393 bp) sequence essentially identical to that of human P-B cDNA (154-546 in D29833) and bovine P-B cDNA (1-356 bp in AB192573) and a sequence of 233 bp (394-626 bp) highly homologous to the segment of E. coli K12 genomic DNA (365511-365744 in NC000913). Although the latter sequence is contained in the vector pT7Blue, which we used, our results show that this chimeric structure in bovine tooth germ P-B cDNA is not an artifact formed during the cloning process, but intrinsic to the bovine genome since the chimeric structure was detected in bovine tooth germ and bovine genomic DNA.
Sato
Ritsuko
R
Department of Dental Hygiene, Nippon Dental University College at Niigata, Niigata 951-8580, Japan.
Isemura
Satoko
S
Fujiwara
Shigeki
S
Sanada
Kazuo
K
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D018076:DNA, Complementary / Q000235:genetics*; D048168:Escherichia coli K12 / Q000235:genetics*; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D055232:Proline-Rich Protein Domains; D012333:RNA, Messenger / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014083:Tooth Germ
D018076:DNA, Complementary; D010455:Peptides; D012333:RNA, Messenger