Application of cryofixation and cryoultramicrotomy for biological electron microscopy.
Conventional chemical fixation and embedding of specimens in resins are accompanied by many artifacts, including postmortem structural alterations. Antigenicity of constituents of specimens can be deteriorated and soluble elements relocated in the process of chemical fixation and resin-embedding. Cryofixation and cryoultramicrotomy will overcome many of these drawbacks of chemical fixation and resin embedding. The theoretical background, equipment, methods, and applications of cryofixation and cryoultramicrotomy for biological specimens are reviewed.
Saga
Kenji
K
Department of Dermatology, Sapporo Medical University School of Medicine, Minami 1 Nishi 16, Chuo-ku, Sapporo, 060-8543, Japan. ksaga@sapmed.ac.jp
D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods; D016616:Cryoultramicrotomy / Q000295:instrumentation / Q000379:methods; D004577:Electron Probe Microanalysis; D006801:Humans; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods; D016253:Microscopy, Immunoelectron / Q000379:methods