title: Granulocyte/macrophage origin of glomerular mesangial cells.
abstract: We previously demonstrated the ability of hematopoietic stem cells (HSCs) to generate glomerular mesangial cells by trans-planting clonal populations of cells derived from a single enhanced green fluorescent protein (EGFP)-positive HSC into lethally irradiated mice. To define more precisely the hematopoietic differentiation pathway through which mesangial cells are derived, we studied the relationship between mesangial cell expression and individual hematopoietic lineages by means of a transplantation strategy. In a series of clonal HSC transplantation experiments, we generated 3 mice engrafted predominantly by granulocytes and macrophages (GMs) and 4 mice engrafted with B-cells or with B-cells and T-cells. When the kidneys of these mice were analyzed, the mice exhibiting high GM lineage engraftment revealed much higher levels of EGFP-positive mesangial cells than those with predominantly lymphocyte engraftment. Fluorescence in situ hybridization analysis of the kidneys from a male recipient of an EGFP-positive female donor excluded cell fusion as the cause for the observed differentiation. These results support the notion that glomerular mesangial cells share their origin with GMs.
authors.affiliation: Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, 29401, USA.
authors.forename: Takanori
authors.initials: T
authors.lastname: Abe
authors.forename: Paul A
authors.initials: PA
authors.lastname: Fleming
authors.forename: Masahiro
authors.initials: M
authors.lastname: Masuya
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Minamiguchi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ebihara
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Drake
authors.forename: Makio
authors.initials: M
authors.lastname: Ogawaa
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000502:physiology / Q000637:transplantation; D002454:Cell Differentiation / Q000502:physiology*; D005260:Female; D005920:Glomerular Mesangium / Q000166:cytology / Q000502:physiology*; D006098:Granulocytes / Q000166:cytology / Q000502:physiology; D018380:Hematopoietic Stem Cell Transplantation; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009000:Monocytes / Q000166:cytology / Q000502:physiology; D023461:Myeloid Progenitor Cells / Q000166:cytology / Q000502:physiology* / Q000637:transplantation; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology / Q000637:transplantation