title: The influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsA.
abstract: The lack of a membrane environment in membrane protein crystals is considered one of the major limiting factors to fully imply X-ray structural data to explain functional properties of ion channels [Gulbis, J.M. and Doyle, D. (2004) Curr. Opin. Struct. Biol. 14, 440-446]. Here, we provide infrared spectroscopic evidence that the structure and stability of the potassium channel KcsA and its chymotryptic derivative 1-125 KcsA reconstituted into native-like membranes differ from those exhibited by these proteins in detergent solution, the latter taken as an approximation of the mixed detergent-protein crystal conditions.
authors.affiliation: Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche, 03202 Alicante, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Encinar
authors.forename: M L
authors.initials: ML
authors.lastname: Molina
authors.forename: J A
authors.initials: JA
authors.lastname: Poveda
authors.forename: F N
authors.initials: FN
authors.lastname: Barrera
authors.forename: M L
authors.initials: ML
authors.lastname: Renart
authors.forename: A M
authors.initials: AM
authors.lastname: Fernández
authors.forename: J M
authors.initials: JM
authors.lastname: González-Ros
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D003902:Detergents / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005960:Glucosides / Q000737:chemistry; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D048170:Streptomyces lividans / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D003902:Detergents; D005960:Glucosides; D010446:Peptide Fragments; D015221:Potassium Channels; D011994:Recombinant Proteins; C107790:prokaryotic potassium channel; C040358:dodecyl maltoside