title: cDNA microarray analysis after laser microdissection in proliferating islets of partially pancreatectomized mice.
abstract: With islet transplantation having grown in popularity since the introduction of the Edmonton protocol, how to secure an unlimited source of islets has become an urgent problem. To resolve this problem, techniques to induce or proliferate islets are urgently required. To achieve this goal, gene expression analysis using a cDNA microarray in islets of partially pancreatectomized mice, in which the remaining islets regenerate and proliferate with insulin secretion and glucose responsiveness, provides us with valuable information. However, those experiments have two critical problems: first, how to selectively collect the regenerating or proliferating islets, and second, the shortage of total RNA extracted from one islet for a microarray analysis. A useful system was thus designed which combined laser microdissection, cDNA amplification by SMART PCR, which can maintain the relative expression profile of transcripts throughout reactions, and a cDNA microarray. Furthermore, this system is expected to contribute to future studies regarding not only islet regeneration but also the function of the islet itself, and this system may also be applicable to many other types of endocrine tissue. In this review, the details of this system are presented and discussed.
authors.affiliation: Laboratory of Molecular Immunology and Diabetes, Department of Health Sciences, School of Medicine, Kyushu University, Higashi-ku, Fukuoka, Japan. katsuta@shs.kyushu-u.ac.jp
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Katsuta
authors.forename: Rimiko
authors.initials: R
authors.lastname: Koyanagi-Katsuta
authors.forename: Masafumi
authors.initials: M
authors.lastname: Shiiba
authors.forename: Keizo
authors.initials: K
authors.lastname: Anzai
authors.forename: Tarou
authors.initials: T
authors.lastname: Irie
authors.forename: Tadateru
authors.initials: T
authors.lastname: Aida
authors.forename: Yuko
authors.initials: Y
authors.lastname: Akehi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nakano
authors.forename: Yohichi
authors.initials: Y
authors.lastname: Yasunami
authors.forename: Mine
authors.initials: M
authors.lastname: Harada
authors.forename: Seiho
authors.initials: S
authors.lastname: Nagafuchi
authors.forename: Junko
authors.initials: J
authors.lastname: Ono
authors.forename: Tetsuhiko
authors.initials: T
authors.lastname: Tachikawa
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D020869:Gene Expression Profiling / Q000379:methods*; D007515:Islets of Langerhans / Q000502:physiology*; D016381:Islets of Langerhans Transplantation; D007834:Lasers; D051379:Mice; D042282:Microdissection; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010180:Pancreatectomy; D012038:Regeneration / Q000235:genetics*; D023822:Tissue Engineering