Detection and characterization of human group C rotaviruses in Bangladesh.
Group C rotaviruses were detected by reverse transcription-PCR in 14 (2.3%) of 611 group A rotavirus-negative stool specimens from the patients admitted to the International Centre for Diarrhoeal Disease Research, Bangladesh hospital, Dhaka, Bangladesh, during July to December 2003. The low rate of detection suggested that infection with group C rotaviruses was an uncommon cause of hospitalization due to gastroenteritis. In addition, coinfections with pathogenic enteric bacteria were frequently observed in group C rotavirus-infected patients. Nucleotide sequence comparison of the VP4, VP6, and VP7 genes revealed that the Bangladeshi group C rotaviruses were most similar to Nigerian group C rotavirus strains. Phylogenetic analysis suggested that all human group C rotaviruses, including the strains isolated in our study, clustered in a monophyletic branch, which was distantly related to the branch comprised of animal group C rotaviruses.
Rahman
Mustafizur
M
ICDDR,B, Centre for Health and Population Research, Mohakhali, Dhaka, Bangladesh. mustafizur.rahman@uz.kuleuven.ac.be
Banik
Sukalyani
S
Faruque
Abu S G
AS
Taniguchi
Koki
K
Sack
David A
DA
Van Ranst
Marc
M
Azim
Tasnim
T
D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000235:genetics; D001459:Bangladesh / Q000453:epidemiology; D036022:Capsid Proteins / Q000235:genetics; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D017422:Sequence Analysis, DNA
D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus