Kinetics of heat-induced whey protein denaturation and aggregation in skim milks with adjusted whey protein concentration.
Microfiltration and ultrafiltration were used to manufacture skim milks with an increased or reduced concentration of whey proteins, while keeping the casein and milk salts concentrations constant. The skim milks were heated on a pilot-scale UHT plant at 80, 90 and 120 degrees C. The heat-induced denaturation and aggregation of beta-lactoglobulin (beta-lg), alpha-lactalbumin (alpha-la) and bovine serum albumin (BSA) were quantified by polyacrylamide gel electrophoresis. Apparent rate constants and reaction orders were calculated for beta-lg, alpha-la and BSA denaturation. Rates of beta-lg, alpha-la and BSA denaturation increased with increasing whey protein concentration. The rate of alpha-la and BSA denaturation was affected to a greater extent than beta-lg by the change in whey protein concentration. After heating at 120 degrees C for 160 s, the concentration of beta-lg and alpha-la associated with the casein micelles increased as the initial concentration of whey proteins increased.
Oldfield
David J
DJ
Whole Protein Technical Centre, Fonterra Co-operative Group Limited, Private Bag 1, Edgecumbe, New Zealand. david.oldfield@fonterra.com
Singh
Harjinder
H
Taylor
Mike W
MW
D000818:Animals; D002364:Caseins / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004220:Disulfides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D007700:Kinetics; D007768:Lactalbumin / Q000737:chemistry; D007782:Lactoglobulins / Q000737:chemistry; D008055:Lipids / Q000032:analysis; D008823:Micelles; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis* / Q000737:chemistry*; D011489:Protein Denaturation; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014462:Ultrafiltration; D000067816:Whey Proteins
D002364:Caseins; D004220:Disulfides; D007782:Lactoglobulins; D008055:Lipids; D008823:Micelles; D008894:Milk Proteins; D000067816:Whey Proteins; D012710:Serum Albumin, Bovine; D007768:Lactalbumin