Insulin-like growth factor-I attenuates the inhibitory effect of type I collagen through beta1 integrin receptor.
Extracellular matrix and growth factors are the crucial factors that regulate healing and regenerating processes in human periodontal ligament cells. The purpose of this study was to examine the effects of type I collagen and insulin-like growth factor-I (IGF-I) on osteopontin (OPN) expression. The data showed that OPN expression was significantly decreased when cells were cultured on collagen-coated plates. Addition of IGF-I obviously induced OPN expression only in a collagen-coated condition, suggesting an attenuating effect of IGF-I on the decrease of OPN expression. Cells treated with a combination of inhibitory antibody to beta1 integrin and IGF-I showed the same level of OPN expression as those treated with either inhibitory antibody to beta1 integrin or IGF-I alone. These results indicate that IGF-I counteracts with the inhibitory signal from type I collagen through beta1 integrin receptor.
Chutivongse
Niraporn
N
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Henri-Dunant Road, Pathumwan, Bangkok 10330, Thailand.
Sumrejkanchanakij
Piyamas
P
Yongchaitrakul
Tussanee
T
Pavasant
Prasit
P
D002478:Cells, Cultured; D024042:Collagen Type I / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D019012:Integrin beta1 / Q000378:metabolism*; D053495:Osteopontin; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000378:metabolism*
D024042:Collagen Type I; D019012:Integrin beta1; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D053495:Osteopontin; D007334:Insulin-Like Growth Factor I