Determination of morphine by molecular imprinting-chemiluminescence method.
A molecular imprinted polymer of morphine was synthesized. Using the imprinted polymer as the recognition material, sodium sulfite as the protective agent, and a potassium permanganate-morphine chemiluminescence system as the detection system, a molecular imprinting-chemiluminescence method for the determination of morphine was established. The linear response range of this method was 5.0 x 10-9-1.0 x 10-6 g/mL (r = 0.9981) and the detection limit was 2 x 10-9 g/mL. The coefficient of variation for 1.0 x 10-7 g/mL morphine solution was 2.8% (n = 9). This method was applied to the determination of morphine in the urine of the heroin abusers with satisfactory results.
He
Yunhua
Y
School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China. ljr@snnu.edu.cn
Lu
Jiuru
J
Liu
Mei
M
Du
Jianxiu
J
Nie
Fei
F
D002138:Calibration; D006801:Humans; D008163:Luminescent Measurements; D009020:Morphine / Q000032:analysis* / Q000652:urine; D011108:Polymers / Q000737:chemistry; D011196:Potassium Permanganate / Q000737:chemistry; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods*; D013447:Sulfites / Q000737:chemistry
D011108:Polymers; D013447:Sulfites; D011196:Potassium Permanganate; D009020:Morphine; C025026:sodium sulfite