Pumping of mammalian cells with a nozzle-diffuser micropump.
We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications.
Yamahata
Christophe
C
Institute of Microelectronics and Microsystems, Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland. christophe.yamahata@a3.epfl.ch
Vandevyver
Caroline
C
Lacharme
Frédéric
F
Izewska
Paulina
P
Vogel
Horst
H
Freitag
Ruth
R
Gijs
Martin A M
MA
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003585:Cell Biology; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D046508:Culture Techniques; D004058:Diffusion; D004867:Equipment Design; D006801:Humans; D006825:Hybridomas / Q000166:cytology; D019169:Jurkat Cells; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D013778:Tetrazolium Salts / Q000494:pharmacology; D013997:Time Factors; D014343:Trypan Blue / Q000494:pharmacology
C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; D013778:Tetrazolium Salts; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D014343:Trypan Blue; D002118:Calcium