Chryseobacterium shigense sp. nov., a yellow-pigmented, aerobic bacterium isolated from a lactic acid beverage.
A yellow-pigmented bacterium designated strain GUM-Kaji(T) was isolated from a lactic acid beverage. The strain had Gram-negative, non-motile, rod-shaped cells. It was strictly aerobic and chemo-organotrophic and grew at 5-30 degrees C and at pH 5-8. The major components of the non-polar and 3-hydroxy fatty acids were C15:0 iso and 3-OH-C17:0 iso, respectively. Menaquinone MK-6 was detected as the sole quinone. 16S rRNA gene sequence comparisons revealed that strain GUM-Kaji(T) is affiliated to the genus Chryseobacterium, with Chryseobacterium joostei as its phylogenetic neighbour, but there were low levels of similarity (<96%) to any established species of the genus. The G+C content of the genomic DNA was 36.6 mol%. The novel bacterium differed from any known species of Chryseobacterium in terms of a number of phenotypic properties. Thus, the name Chryseobacterium shigense sp. nov. is proposed for this novel bacterium. The type strain is strain GUM-Kaji(T) (=BAMY 1001(T)=NCIMB 14047(T)=DSM 17126(T)).
Shimomura
Kengo
K
NCIMB Japan Co., Ltd, Shimizunagasaki, Shizuoka-shi, Shizuoka 424-0065, Japan.
Kaji
Shigeo
S
Yumai Co., Ltd, Kouga-gun, Shiga 529-1835, Japan.
Hiraishi
Akira
A
Department of Ecological Engineering, Toyohashi University of Technology, Toyohashi 441-8580, Japan.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001628:Beverages / Q000382:microbiology*; D045247:Chryseobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D019344:Lactic Acid; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid