title: [A patient with Legionella pneumophila serogroup-3 pneumonia, detected by PCR].
abstract: A 59-year-old man was hospitalised because of dyspnoea, productive cough, fever, chills and malaise. Severe community-acquired pneumonia was diagnosed. Legionella urinary antigen testing, which can only detect serogroup 1, and the first culture ofa bronchoalveolar lavage (BAL) fluid sample were negative for Legionella. However, L. pneumophila DNA was detected by PCR in the BAL washing sample. Eventually, L. pneumophila serogroup 3 was isolated from this specimen by repeated culture. Although, in The Netherlands, legionellosis is caused by L. pneumophila serogroup 1 in more than 90% of all cases, this case demonstrates that a negative result of urinary antigen testing does not necessarily exclude this diagnosis. It is therefore advocated to expand the diagnostics to a Legionella PCR on respiratory material of patients with clinical signs of Legionella pneumonia in whom the urinary antigen test is negative.
authors.affiliation: St. Antonius Ziekenhuis, afd. Medische Microbiologie en Immunologie, Nieuwegein. b.herpers@antonius.net
authors.forename: B L
authors.initials: BL
authors.lastname: Herpers
authors.forename: A W J
authors.initials: AW
authors.lastname: Bossink
authors.forename: J W
authors.initials: JW
authors.lastname: Cohen Stuart
authors.forename: W N M
authors.initials: WN
authors.lastname: Hustinx
authors.forename: S F T
authors.initials: SF
authors.lastname: Thijsen
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D017714:Community-Acquired Infections / Q000175:diagnosis; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping
chemical_list: D004269:DNA, Bacterial