Functional studies on a ketoreductase gene from Streptomyces sp. AM-7161 to control the stereochemistry in medermycin biosynthesis.
Medermycin shows the same trans (3S,15R) configuration as actinorhodin in the pyran ring crucial for its bioactivity. One medermycin biosynthetic gene, med-ORF12, is assumed to be involved in the stereochemical control at C-3. Functional complementation suggested that it plays a similar role as actVI-ORF1 previously proved to determine the stereospecificity at C-3 in actinorhodin biosynthesis. Co-expression of med-ORF12 with actinorhodin early biosynthetic genes further demonstrated that med-ORF12 encodes a ketoreductase responsible for the enantioselective reduction at C-3 in the formation of the pyran ring.
Li
Aiying
A
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Tokyo 113-0033, Japan.
Itoh
Takayuki
T
Taguchi
Takaaki
T
Xiang
Ting
T
Ebizuka
Yutaka
Y
Ichinose
Koji
K
D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D015394:Molecular Structure; D005810:Multigene Family; D009285:Naphthoquinones / Q000737:chemistry / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D013237:Stereoisomerism; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D000095622:Benzoisochromanequinones
D000429:Alcohol Oxidoreductases; D009285:Naphthoquinones; C013104:medermycin; D000095622:Benzoisochromanequinones