Thermincola carboxydiphila gen. nov., sp. nov., a novel anaerobic, carboxydotrophic, hydrogenogenic bacterium from a hot spring of the Lake Baikal area.
A novel anaerobic, thermophilic, alkalitolerant bacterium, strain 2204(T), was isolated from a hot spring of the Baikal Lake region. The cells of strain 2204(T) were straight rods of variable length, Gram-positive with an S-layer, motile with one to two lateral flagella, and often formed aggregates of 3-15 cells. The isolate was shown to be an obligate anaerobe oxidizing CO and producing equimolar quantities of H(2) and CO(2) according to the equation CO+H(2)O-->CO(2)+H(2). No organic substrates were used as energy sources. For lithotrophic growth on CO, 0.2 g acetate or yeast extract l(-1) was required but did not support growth in the absence of CO. Growth was observed in the temperature range 37-68 degrees C, the optimum being 55 degrees C. The pH range for growth was 6.7-9.5, the optimum pH being 8.0. The generation time under optimal conditions was 1.3 h. The DNA G+C content was 45 mol%. Penicillin, erythromycin, streptomycin, rifampicin, vancomycin and tetracycline completely inhibited both growth and CO utilization by strain 2204(T). Thus, isolate 2204(T) was found to be the first known moderately thermophilic and alkalitolerant H(2)-producing anaerobic carboxydotroph. The novel bacterium fell within the cluster of the family Peptococcaceae within the low-G+C-content Gram-positive bacteria, where it formed a separate branch. On the basis of morphological, physiological and phylogenetic features, strain 2204(T) should be assigned to a novel genus and species, for which the name Thermincola carboxydiphila gen. nov., sp. nov. is proposed. The type strain is strain 2204(T) (=DSM 17129(T)=VKM B-2283(T)=JCM 13258(T)).
Sokolova
Tatyana G
TG
Institute of Microbiology, Russian Academy of Sciences, Prospekt 60, let Oktyabrya 7/2, 117811 Moscow, Russia.
Kostrikina
Nadezhda A
NA
Institute of Microbiology, Russian Academy of Sciences, Prospekt 60, let Oktyabrya 7/2, 117811 Moscow, Russia.
Chernyh
Nikolai A
NA
Institute of Microbiology, Russian Academy of Sciences, Prospekt 60, let Oktyabrya 7/2, 117811 Moscow, Russia.
Kolganova
Tatjana V
TV
Institute of Microbiology, Russian Academy of Sciences, Prospekt 60, let Oktyabrya 7/2, 117811 Moscow, Russia.
Tourova
Tatjana P
TP
Institute of Microbiology, Russian Academy of Sciences, Prospekt 60, let Oktyabrya 7/2, 117811 Moscow, Russia.
Bonch-Osmolovskaya
Elizaveta A
EA
Institute of Microbiology, Russian Academy of Sciences, Prospekt 60, let Oktyabrya 7/2, 117811 Moscow, Russia.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D002248:Carbon Monoxide / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D045482:Hot Springs / Q000382:microbiology*; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests / Q000379:methods; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010459:Peptococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002248:Carbon Monoxide; D006859:Hydrogen