Influence of different adhesive restorative materials on mutans streptococci colonization.
OBJECTIVE
To evaluate the effect of some restorative materials on mutans streptococci colonization.


METHODS
Awild strain of mutans streptococci was isolated from human dental plaque and a monospecific biofilm adherent to the surfaces of 12 adhesive restorative materials (F2000, Dyract AP, Compoglass F, Z100 MP, Filtek Z250, Clearfil, Ketac-Bond, Ketac-Fil Plus, Ketac-Molar, Fuji Cap II, Fuji Bond LC and Fuji II LC) were tested. A colorimetric technique (MTT assay), based on the reduction of a yellow tetrazolium salt to a purple formazan, was used to evaluate the biomass adherent to the disk surfaces after a 24-hour growth.


RESULTS
One-way ANOVA showed that glass-ionomer cements (Fuji Cap II, Ketac-Bond, Ketac-Fil and Ketac-Molar) had similar antibacterial effects on mutans streptococci and were significantly more effective (P < 0.001) than all other products tested in reducing the biofilm development on their surfaces. The compomers (Dyract, Compoglass F and F 2000) showed a significantly higher value of bacterial colonization than all the other materials (P < 0.001).
Brambilla
Eugenio
E
University of Milan, Department of Medicine, Surgery and Dentistry San Paolo, Via Beldiletto 1/3, 20142 Milan, Italy. Eugenio.Brambilla@unimi.it
Cagetti
Maria Grazia
MG
Gagliani
Massimo
M
Fadini
Luigi
L
García-Godoy
Franklin
F
Strohmenger
Laura
L
D000269:Adhesives / Q000494:pharmacology*; D000704:Analysis of Variance; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects; D020770:Compomers / Q000494:pharmacology*; D003188:Composite Resins / Q000494:pharmacology*; D003773:Dental Plaque / Q000382:microbiology; D003793:Dental Restoration, Permanent; D005899:Glass Ionomer Cements / Q000494:pharmacology*; D006801:Humans; D013295:Streptococcus mutans / Q000187:drug effects*
D000269:Adhesives; D020770:Compomers; D003188:Composite Resins; D005899:Glass Ionomer Cements