Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China.
A spore-forming, halophilic bacterium, designated strain BH163(T), was isolated from a salt lake in China. Cells were motile, strictly aerobic rods that contained type A1gamma peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. The isolate showed Gram- and catalase-positive reactions and formed a terminal endospore with a swollen sporangium. The major cellular fatty acids were anteiso-C(15:0), iso-C(15:0), anteiso-C(17:0) and iso-C(16:0). The genomic DNA G+C content of the strain was 41.0 mol%. Comparative analysis of 16S rRNA gene sequences showed that strain BH163(T) formed a distinct line within the phyletic group classically defined as the genus Bacillus and was most closely related to the taxa [Bacillus] haloalkaliphilus DSM 5271(T) and Filobacillus milosensis DSM 13259(T), with 16S rRNA gene sequence similarities of 95.9 and 94.5%, respectively. On the basis of physiological and molecular properties, it is proposed that [Bacillus] haloalkaliphilus DSM 5271(T) is reclassified in the new genus Alkalibacillus as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. Strain BH163(T) (=KCTC 3916(T)=DSM 16460(T)) was assigned as the type strain of the novel species Alkalibacillus salilacus.
Jeon
Che Ok
CO
Environmental Biotechnology National Core Research Center, Division of Environmental Biotechnology, Gyeongsang National University, 660-701, Korea.
Lim
Jee-Min
JM
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Lee
Jung-Min
JM
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
Xu
Li-Hua
LH
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Jiang
Cheng-Lin
CL
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Kim
Chang-Jin
CJ
Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Republic of Korea.
D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride