title: Mutator phenotype resulting from DNA polymerase IV overproduction in Escherichia coli: preferential mutagenesis on the lagging strand.
abstract: We investigated the mutator effect resulting from overproduction of Escherichia coli DNA polymerase IV. Using lac mutational targets in the two possible orientations on the chromosome, we observed preferential mutagenesis during lagging strand synthesis. The mutator activity likely results from extension of mismatches produced by polymerase III holoenzyme.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02106 Warsaw, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kuban
authors.forename: Magdalena
authors.initials: M
authors.lastname: Banach-Orlowska
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Bialoskorska
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Lipowska
authors.forename: Roel M
authors.initials: RM
authors.lastname: Schaaper
authors.forename: Piotr
authors.initials: P
authors.lastname: Jonczyk
authors.forename: Iwona J
authors.initials: IJ
authors.lastname: Fijalkowska
mesh_terms: D004258:DNA Polymerase III / Q000235:genetics; D019951:DNA Polymerase beta / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D016296:Mutagenesis / Q000235:genetics*; D010641:Phenotype
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III; D019951:DNA Polymerase beta; C468316:dnaQ protein, E coli