Marinococcus halotolerans sp. nov., isolated from Qinghai, north-west China.
An aerobic bacterium was isolated from saline soil located in Qinghai, north-west China. The bacterium, designated YIM 70157(T), was investigated using a polyphasic taxonomic approach. The Gram reaction of the organism was positive. Comparative 16S rRNA gene sequence analysis demonstrated the isolate to be a member of the genus Marinococcus, the closest phylogenetic neighbour of the unknown bacterium being Marinococcus halophilus DSM 20408(T) with a similarity of 99.4%. The peptidoglycan type of YIM 70157(T) was A1gamma, with meso-diaminopimelic acid as diagnostic diamino acid. The major fatty acids were ai-C(15:0), ai-C(17:0) and i-C(16:0). The menaquinones were MK-7 and MK-6. The phospholipids were diphosphatidylglycerol and phosphatidylinositol. The G+C content of total DNA was 48.5 mol%. On the basis of phylogenetic and phenotypic evidence and DNA-DNA hybridization data, this isolate should be classified as a novel species of Marinococcus, for which the name Marinococcus halotolerans sp. nov. is proposed. The type strain is YIM 70157(T) (=DSM 16375(T)=KCTC 19045(T)).
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Schumann
Peter
P
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Zhang
Yu-Qin
YQ
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Chen
Guo-Zhong
GZ
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Tian
Xin-Peng
XP
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Xu
Li-Hua
LH
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Stackebrandt
Erko
E
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Jiang
Cheng-Lin
CL
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D020459:Genes, rRNA; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D012988:Soil Microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride