99mTc-labeled 1-thio-beta-D-glucose as a new tumor-seeking agent: synthesis and tumor cell uptake assay.
99mTc-labeled 1-thio-beta-D-glucose (99mTc-1-TG) was synthesized under different ligand concentrations. 1-Thio-beta-D-glucose (1-TG) showed different chemical properties and higher stability at acid pH as compared to neutral and basic pH. Under different ligand concentrations, 99mTc-1-TG was obtained with high labeling efficiency of >97%, but the HPLC chromatogram varied with the ligand concentration. The results of a tumor cell uptake assay also depended on the ligand concentration. With the lowest ligand concentration tested (0.5 mg/ml of 1-TG), we obtained the highest uptake. With different glucose and insulin concentrations, 99mTc-1-TG uptake was not significantly different from that of [18F]FDG.
Jun Oh
Seung
S
Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, 388-1 Pungnap-dong, Songpa-gu, Seoul 138-736, Republic of Korea. sjoh@amc.seoul.kr
Ryu
Jin-Sook
JS
Yoon
Eun-Jin
EJ
Bae
Mun Sun
MS
Choi
Sun Joo
SJ
Park
Kyung Bae
KB
Moon
Dae Hyuk
DH
D001932:Brain Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D015179:Colorectal Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism; D005947:Glucose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling / Q000379:methods*; D008175:Lung Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D013667:Technetium / Q000737:chemistry*
D019275:Radiopharmaceuticals; D013667:Technetium; C018399:1-thioglucose; D005947:Glucose