[Metalloproteinase (MMP-1, 2 and 11), tissue inhibitor of metalloproteinase-1 (TIMP-1), and p53 expression in nasal-type angiocentric T/NK-cell lymphoma: an immunohistochemical study].
Twenty cases of extraganglionar Nasal-type T/NK-cell lymphomas were analyzed at the National Cancer Institute of Mexico. We studied immunophenotype of neoplastic cells, nuclear p53 expression, and enzymes as matrix metalloplroteinases participating in invasion, tissular destruction and metastases.


METHODS
Paraffin blocks from all cases were retrieved and analyzed by hermatoxilin and eosin. Histopathological features included cellular size and cytologic characteristics. We performed immunohistochemistry to determine CD3, CD56, p53 cellular type and expression of (MMPs-1, 2, 11) matrix metalloproteinases and one tissue inhibitor of TIMP-1 metalloproteinase. Demographic variables included, age, sex, primaony location, clinical stage, treatment and follow-up. STATISTIC ALANALYSIS: The association of different matrix metalloproteinases in epthial and tumoral cells, stroma, necrosis and endothelial cells were found to be significant using Fisher's exact test.


RESULTS
All studied cases were positive to cytoplasmic CD3, CD56 (NK cells), 19 of them were positive to p53, five of them with nuclear overexpression of p53 in more than 50% of neoplastic cells. There was significant expression of MMP-1 in tumoral cells; the epithelium displayed significant expression of TIMP-1 and MMP-11. Patients with p53 overexpression displayed a poorer prognosis. Three of them had undergone radiotherapy and died within the first month of treatment.


CONCLUSIONS
This type of lymphoma is a common neoplasm in Asia, Latin America and Mexico. It is worth noting it has has been linked to Epstein-Barr virus with T/NK-cell phenotype, which often displays cellular atypia, an angiocentric growth pattern and necrosis. It is clinically expressed by progressive destruction of midline facial soft tissue and has a poor prognosis.
Meneses-García
Abelardo
A
Instituto Nacional de Cancerología, DF, México. aameneses@hotmail.com
Herrera
Jorge
J
Mohar
Alejandro
A
García-Cuellar
Claudia
C
Súchil-Bernal
Laura
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003627:Data Interpretation, Statistical; D005260:Female; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020782:Matrix Metalloproteinases; D045726:Metalloproteases / Q000378:metabolism*; D008875:Middle Aged; D009296:Nasal Cavity; D009303:Nasopharyngeal Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009669:Nose Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010157:Palatal Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53
D019715:Tissue Inhibitor of Metalloproteinase-1; D016159:Tumor Suppressor Protein p53; D045726:Metalloproteases; D020782:Matrix Metalloproteinases