title: Vascular endothelial growth factor (VEGF) in human tooth germ center.
abstract: Many oncogenis and tumour suppressor genes found inside normal and pathological cells are fundamental for the processes of development, proliferation and tissue differentiation. The purpose of our study is to show the presence and a possible relationship of the VEGF protein during different phases of the development of human dental germ centers. After cephalometric investigation in 8 orthodontic patients with a mean age of 13 years, (4 females and 4 males), hyperdivergence of the third molars were extracted. The 40 surgical samples were tested with monoclonal human anti-VEGFs antibodies carrying out a semi-quantitative analysis to look for a positive reaction. Reaction for anti-VEGF antibodies was detected in normal embryological tissues and in microvessels near odontogenic cells. During different phases of embryologic development of the dental bud our search showed intracytoplasmatic positive immunoreactions both in the ameloblastic and odontoblastic cells. Additionally, a positive reaction was observed for the VEGF protein in the cells of the stellate reticulum and in those endothelial tissue surrounding the microvessels in all the samples examined.
authors.affiliation: Oral Surgery of Oral Science Department, University G. d'Annunzio, Chieti, Italy. filibertomastrangelo@hotmail.com
authors.forename: F
authors.initials: F
authors.lastname: Mastrangelo
authors.forename: M
authors.initials: M
authors.lastname: Piccirilli
authors.forename: M
authors.initials: M
authors.lastname: Dolci
authors.forename: S
authors.initials: S
authors.lastname: Teté
authors.forename: L
authors.initials: L
authors.lastname: Speranza
authors.forename: A
authors.initials: A
authors.lastname: Patruno
authors.forename: F
authors.initials: F
authors.lastname: Gizzi
authors.forename: M
authors.initials: M
authors.lastname: Felaco
authors.forename: L
authors.initials: L
authors.lastname: Artese
authors.forename: M A
authors.initials: MA
authors.lastname: De Lutiis
mesh_terms: D000293:Adolescent; D000328:Adult; D000565:Ameloblasts / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015153:Blotting, Western; D002648:Child; D003720:Densitometry; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D018919:Neovascularization, Physiologic; D009804:Odontoblasts / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014083:Tooth Germ / Q000098:blood supply / Q000737:chemistry* / Q000166:cytology / Q000196:embryology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A