[An experiment study of protection and treatment of Kudan granule on rats of pulmonary fibrosis induced by pinyangmycin].
OBJECTIVE
To explore the protection and treatment effects of Kudan granule on rats of pulmonary fibrosis induced by pinyangmycin.


METHODS
In asepsis condition, rat was anaesthetized by 3.5% chloral hydrate, inserted the needle above the bifurcation of trachea and injecting 5 mg x kg(-1) pinyangmycin normal saline solution.


RESULTS
For model-group rats, after injecting pinyangmycin at 7, 14, 28, 56 days, there were mass inflammation cell infiltration at pulmonary alveoli and interstitial, the pulmonary alveolar wall and interstitial thickened obviously, and the pulmonary interval broadened distinctly. The structure of collagen fiber was destroyed, and the pulmonary alveoli disappeared. By Masson dyeing, there were hunk collagen fiber and the consolidation of lung had come into being. Compared with the model group, the rats of Kudan granule big-dose group, at 7, 14, 28, 56 days after injecting pinyangmycin, there were still much inflammation cell infiltration at pulmonary alveoli and interstitial, pulmonary interstitial edema and spotty necrosis, thickened alveolar wall, broadened pulmonary interval, and much collagen fiber in pulmonary interstitial, but there was not bunk the consolidation of lung. The curative effect of Kudan granule small-dose group was not better than that of Kudan granule big-dose group, because there were still bunk collagen fiber and the consolidation of lung in pulmonary interstitial. Although the destroyed area was more than 50%, it was better than that of the model-group.


CONCLUSIONS
The big-dose Kudan granule show the better function of protection and treatment for pulmonary fibrosis of rats induced by pinyangmycin.
Song
Chong-shun
CS
The Dongzhimen Hospital Affiliated to Beijing University of Traditional Chinese Medicine, Beijing 100700, China. songlchyl@yahoo.com.cn
Ren
Ying
Y
Yin
Jun-xiang
JX
Sun
Hai-yun
HY
Song
Jing-jing
JJ
Cui
Jie-feng
JF
Tan
Wei
W
Guo
Jing
J
Dai
Xin
X
Zhao
Feng-zhi
FZ
Yang
Jin-duo
JD
D000818:Animals; D001761:Bleomycin / Q000031:analogs & derivatives; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D005260:Female; D008168:Lung / Q000473:pathology; D008297:Male; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry; D029909:Sophora / Q000737:chemistry
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D001761:Bleomycin; C021771:zhengguangmycin