Effect of foot shock stress on the interferon-gamma production of murine intestinal intraepithelial lymphocytes.
To investigate the effect of stress on interferon (IFN)-gamma production by intestinal intraepithelial lymphocytes (IEL), we exposed male C3H/HeN mice to electric foot shock for 30 min a day for 5 consecutive days. Immediately after the final foot shock stress, IEL from small intestine were isolated by Percoll density gradient. The stress induced a marked suppression of IFN-gamma production by IEL stimulated with immobilized anti-CD3 mAb and a marked decrease in the proportion of IFN-gamma-producing CD3+ IEL or alphabetaTCR+ IEL stimulated with PMA+ionomycin. The alphabetaTCR+ subset was the major cause of stress-induced suppression of IFN-gamma production by IEL. Glucocorticoid induced the suppression of IFN-gamma production by IEL in vitro, which was reversed by mifepristone (RU486), a glucocorticoid receptor antagonist. In vivo administration of RU486 reversed the stress-induced suppression of IFN-gamma production by IEL. In conclusion, repeated foot shock stress suppressed IFN-gamma production of IEL by stress-induced elevation of endogenous glucocorticoid. Substantial suppression of the alphabetaTCR+ subset was the major cause of the suppression.
Zhang
Xiumin
X
Department of Medicine and Science in Sports and Exercise, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, 980-8575 Sendai, Japan. xmzhang@mail.tains.tohoku.ac.jp
Okutsu
Mitsuharu
M
Kanemi
Osamu
O
Nagatomi
Ryoichi
R
D000818:Animals; D003345:Corticosterone / Q000097:blood; D003907:Dexamethasone / Q000097:blood; D004597:Electroshock; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology*; D007421:Intestine, Small / Q000276:immunology; D015759:Ionomycin; D007476:Ionophores; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D015735:Mifepristone / Q000008:administration & dosage / Q000494:pharmacology; D016853:Muromonab-CD3; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis / Q000276:immunology*; D011965:Receptors, Glucocorticoid / Q000037:antagonists & inhibitors; D013312:Stress, Physiological / Q000209:etiology / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate
D007476:Ionophores; D016853:Muromonab-CD3; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011965:Receptors, Glucocorticoid; D015735:Mifepristone; D015759:Ionomycin; D003907:Dexamethasone; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate; D003345:Corticosterone