Expression of the adrenomedullin gene in adipose tissue.
Adrenomedullin (AM) is a potent vasodilating peptide originally isolated from human pheochromocytoma cells. This report concerns the expression and secretion of AM from adipose tissue. Northern blot analysis demonstrated marked expression of AM mRNA in mouse adipose tissue. Expression levels in adipose tissues were 2.5-3.2 times higher than in the kidney. AM mRNA level in mature adipocytes was 7.3 times higher than in the stroma-vascular fraction of adipose tissue. In mature adipocyte culture, time-dependent increase of AM peptide concentration in the culture medium was detected. AM expression was also detected in human subcutaneous adipose tissue. Adipose AM expression significantly increased in obesity mouse model, high-fat diet fed mice and ob/ob mice. These results suggest that adipose tissue, especially mature adipocytes, is major source of AM in the body, and that adipocyte-derived AM plays a pathophysiological role in obesity.
Nambu
Takuo
T
Department of Medicine and Clinical Science Kyoto University Graduate School of Medicine 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
Arai
Hiroshi
H
Komatsu
Yasato
Y
Yasoda
Akihiro
A
Moriyama
Kenji
K
Kanamoto
Naotetsu
N
Itoh
Hiroshi
H
Nakao
Kazuwa
K
D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D053607:Adrenomedullin; D000818:Animals; D015992:Body Mass Index; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000378:metabolism*; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
D010455:Peptides; D012333:RNA, Messenger; D053607:Adrenomedullin