title: Enterococcus canintestini sp. nov., from faecal samples of healthy dogs.
abstract: The taxonomic position of strain LMG 13590(T), originally isolated from dog faeces and classified as Enterococcus dispar in the BCCM/LMG Bacteria Catalogue, was reinvestigated. This strain and 12 recent isolates from faecal samples of healthy dogs occupied a clearly separate position when investigated with multilocus sequence analysis (MLSA) of the genes encoding the alpha subunit of ATP synthase (atpA), RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS). The 16S rRNA gene sequence of one representative strain showed highest similarities of 98-99% with E. dispar LMG 13521(T), Enterococcus canis LMG 12316(T) and Enterococcus asini LMG 18727(T). A further polyphasic taxonomic study based on whole-cell protein fingerprinting, DNA-DNA hybridization and biochemical features demonstrated that the 13 enterococcal dog faecal strains represent a single, novel Enterococcus species for which the name Enterococcus canintestini sp. nov. is proposed. The type strain is LMG 13590(T) (=CCM 7285(T)).
authors.affiliation: BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.|Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
authors.forename: Sabri M
authors.initials: SM
authors.lastname: Naser
authors.affiliation: BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Vancanneyt
authors.affiliation: Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Evelyne
authors.initials: E
authors.lastname: De Graef
authors.affiliation: Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Luc A
authors.initials: LA
authors.lastname: Devriese
authors.affiliation: BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
authors.forename: Cindy
authors.initials: C
authors.lastname: Snauwaert
authors.affiliation: BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
authors.forename: Karen
authors.initials: K
authors.lastname: Lefebvre
authors.affiliation: BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Hoste
authors.affiliation: Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Švec
authors.affiliation: Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Annemie
authors.initials: A
authors.lastname: Decostere
authors.affiliation: Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
authors.forename: Freddy
authors.initials: F
authors.lastname: Haesebrouck
authors.affiliation: BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.|Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004285:Dogs / Q000382:microbiology*; D016983:Enterococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S