title: Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant.
abstract: Yellow-pigmented, Gram-negative organisms isolated from raw chicken were investigated by means of a polyphasic taxonomic approach and were shown to represent a novel species in the genus Chryseobacterium, for which the name Chryseobacterium vrystaatense sp. nov. is proposed. Its nearest phylogenetic neighbours were Chryseobacterium joostei, Chryseobacterium indologenes and Chryseobacterium gleum, which showed 16S rRNA gene sequence similarity levels of 96.9, 97.1 and 96.1%, respectively. Levels of DNA-DNA hybridization between strains of C. vrystaatense and Chryseobacterium reference species were below 46%. Strain LMG 22846(T) (=CCUG 50970(T)) was chosen as the type strain and has a DNA G+C content of 37.1 mol%.
authors.affiliation: School for Agriculture and Environmental Science, Central University of Technology: Free State, P/bag x20539, 1 Pres. Brandt Street, Bloemfontein 9300, South Africa.
authors.forename: Hanli
authors.initials: H
authors.lastname: de Beer
authors.affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa.
authors.forename: Celia J
authors.initials: CJ
authors.lastname: Hugo
authors.affiliation: Department of Biotechnology and Food Technology, Tshwane University of Technology, Pretoria, South Africa.
authors.forename: Piet J
authors.initials: PJ
authors.lastname: Jooste
authors.affiliation: Laboratorium of Microbiology, Ghent University, K.L. Ledeganckstraat 35, Ghent B-9000, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Willems
authors.affiliation: BCCM/LMG Bacteria Collection, Ghent University, K.L. Ledeganckstraat 35, Ghent B-9000, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Vancanneyt
authors.affiliation: Laboratorium of Microbiology, Ghent University, K.L. Ledeganckstraat 35, Ghent B-9000, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Coenye
authors.affiliation: Laboratorium of Microbiology, Ghent University, K.L. Ledeganckstraat 35, Ghent B-9000, Belgium.
authors.forename: Peter A R
authors.initials: PAR
authors.lastname: Vandamme
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002645:Chickens / Q000382:microbiology*; D045247:Chryseobacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005511:Food Handling / Q000379:methods*; D005525:Food-Processing Industry; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S