Comparative validation study to demonstrate the equivalence of a minor modification to AOAC methods 996.09, Vip for EHEC and 996.10, assurance Eia EHEC with the reference culture method for the detection of Escherichia coli O157:H7 in beef.
The Visual Immunoprecipitate Assay (VIP) method for the detection of enterohemorrhagic Escherichia coli O157:H7 (VIP for EHEC) and Assurance Enzyme Immunoassay (EIA) method for the detection of EHEC (EHEC EIA) are AOAC INTERNATIONAL Official Methods 996.09 and 996.10, respectively. A minor modification to the enrichment medium used in both methods has been developed. This modification, the BioControl modified EHEC medium (BioControl mEHEC) provides a more cost-effective procedure with performance equivalent to that of the cultural method for detection of E. coli O157:H7 in beef.
Feldsine
Philip T
PT
BioControl Systems, Inc., 12822 SE 32nd St, Bellevue, WA 98005, USA. ptf@biocontrolsys.com
Green
Shannon T
ST
Lienau
Andrew H
AH
Kerr
David E
DE
D000818:Animals; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards*; D003470:Culture Media; D019453:Escherichia coli O157 / Q000378:metabolism*; D005504:Food Analysis; D005506:Food Contamination; D005516:Food Microbiology; D007124:Immunoenzyme Techniques; D008460:Meat / Q000382:microbiology*; D012015:Reference Standards; D015203:Reproducibility of Results
D003470:Culture Media