title: Inhibitory effect of some selected nutraceutic herbs on LDL glycation induced by glucose and glyoxal.
abstract: Anti-LDL glycative agents were investigated using aqueous extracts of Psidium guajava L. (PE), Toona sinensis Roem. (TE), Momordica charantia L. (ME) and Graptopetalum paragugayene E. Walther (GE). Concentrations of extracts 0.01-0.625 mg/mL, low density lipoprotein (LDL; 100 microg protein/mL) and inducers glucose (400 mM) and glyoxal (2.5 mM) were incubated at 37 degrees C. Evaluation parameters involved the thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD), relative electrophoretic mobility (REM), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capability and total polyphenolic content. Results for anti-TBARS efficiency (in%) were PE (75.77), TE (75.10), ME (68.81) and GE (19.81) at 0.5 mg/mL, respectively, when induced by glucose; 36.68, 35.60, 32.62 and inactive, respectively, by glyoxal. The lag times for CD formation (in min) were: 289 and 125 by PE and TE, respectively, comparing to the control (45). REM was 1.6 with respect to PE (0.1 mg/mL) compared to the control (4.2). PE at 0.01 mg/mL effectively inhibited with 63.45% efficiency on AGEs induced by glucose. We conclude that PE virtually is a potent antiglycative agent, which can be of great value in the preventive glycation-associated cardiovascular and neurodegenerative diseases.
authors.affiliation: Department of Food and Nutrition, Shalu County, Taichung, Taiwan, ROC.
authors.forename: Chiu-Lan
authors.initials: CL
authors.lastname: Hsieh
authors.forename: Yuh-Charn
authors.initials: YC
authors.lastname: Lin
authors.forename: Wang-Sheng
authors.initials: WS
authors.lastname: Ko
authors.forename: Chiung-Hui
authors.initials: CH
authors.lastname: Peng
authors.forename: Chien-Ning
authors.initials: CN
authors.lastname: Huang
authors.forename: Robert Y
authors.initials: RY
authors.lastname: Peng
mesh_terms: D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006031:Glycosylation; D006037:Glyoxal / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D010946:Plants, Medicinal; D059808:Polyphenols; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D005419:Flavonoids; D016166:Free Radical Scavengers; D017127:Glycation End Products, Advanced; D007004:Hypoglycemic Agents; D008077:Lipoproteins, LDL; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D017392:Thiobarbituric Acid Reactive Substances; D006037:Glyoxal; D005947:Glucose