Human placental immunoglobulins show unique re-association patterns with isologous and third party acid treated trophoblast microvesicles in vitro.
OBJECTIVE
To study re-association pattern of human placental eluate immunoglobulins with acid treated isologous and third party trophoblast derived placental microvesicles.


METHODS
Laboratory based experimentation.


METHODS
Biological Sciences Department and Discipline for Reproductive Medicine University of Newcastle, Australia and the Department of Biochemistry, University of Nairobi, Kenya.


RESULTS
Placental eluate immunoglobulins re-associated with isologous and third party acidified microvesicles in three distinct patterns. I: eluate immunoglobulins re-associated more strongly with isologous and third party acid treated placental microvesicles, II: eluate immunoglobulins re-associated strongly with isologous but weakly with third party acid treated placental microvesicles, III: eluate immunoglobulins did not show preferential re-association with isologous and third party acid treated placental microvesicles.


CONCLUSIONS
Two types of antigenic epitopes I and II may be expressed on the human placentae. Type I antigens may be present on all human placentae while type II epitopes may be paternally derived hence unique to each pregnancy. Also, immunoglobulins produced to placental microvesicle antigens may be directed to some but not all antigenic epitopes expressed on the human placental trophoblast.
Omwandho
C A
CA
Department of Biochemistry, College of Health Sciences, University of Nairobi, P.O. Box 30197. Nairobi, Kenya.
Falconer
J
J
Gruessner
S E
SE
Mecha
E
E
Tumbo-Oeri
A G
AG
Roberts
T K
TK
Tinneberg
H R
HR
D000143:Acids / Q000494:pharmacology*; D000941:Antigens / Q000276:immunology* / Q000302:isolation & purification; D001769:Blood; D000939:Epitopes / Q000276:immunology*; D005260:Female; D005327:Fetal Resorption; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology* / Q000302:isolation & purification; D066298:In Vitro Techniques; D008431:Maternal-Fetal Exchange / Q000276:immunology; D010920:Placenta / Q000276:immunology*; D011247:Pregnancy / Q000276:immunology*; D014327:Trophoblasts / Q000276:immunology*
D000143:Acids; D000941:Antigens; D000939:Epitopes; D007136:Immunoglobulins