title: Peyer patches are not required for acute graft-versus-host disease after myeloablative conditioning and murine allogeneic bone marrow transplantation.
abstract: Graft-versus-host disease (GVHD) is a multistep disease process following allogeneic bone marrow transplantation (BMT). It has been postulated that the induction of acute GVHD requires the presence of Peyer patches (PPs). A new tumor necrosis factor (TNF)-deficient strain has been developed that totally lacks PPs and displays the defects characteristic of TNF ablation but not lymphotoxin-associated defects characterized by lack of both PPs and lymph nodes. To determine the necessity of PPs in acute lethal GVHD induction, we transplanted full major histocompatibility complex (MHC)-mismatched grafts into myeloablated TNF knockout recipients. No differences in the survival or GVHD-associated histopathologic lesions were observed between the recipients. We conclude that neither PPs nor host TNF-alpha is required for the development of acute lethal GVHD in mice that undergo myeloablative conditioning and allogeneic BMT.
authors.affiliation: Department of Microbiology and Immunology, University of Nevada School of Medicine, Mail Stop 199, Reno, NV 89557, USA. lwelniak@unr.edu
authors.forename: Lisbeth A
authors.initials: LA
authors.lastname: Welniak
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Kuprash
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Tumanov
authors.forename: Angela
authors.initials: A
authors.lastname: Panoskaltsis-Mortari
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Blazar
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Nedospasov
authors.forename: William J
authors.initials: WJ
authors.lastname: Murphy
mesh_terms: D000208:Acute Disease; D000818:Animals; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D006086:Graft vs Host Disease / Q000209:etiology* / Q000473:pathology; D007410:Intestinal Diseases / Q000209:etiology; D051379:Mice; D018345:Mice, Knockout; D010581:Peyer's Patches / Q000502:physiology*; D019172:Transplantation Conditioning / Q000379:methods; D014184:Transplantation, Homologous; D014409:Tumor Necrosis Factor-alpha / Q000172:deficiency
chemical_list: D014409:Tumor Necrosis Factor-alpha