Blunted inflammatory response to lipopolysaccharide endotracheal instillation due to thermal preconditioning.
OBJECTIVE
Heat shock proteins (HSPs), induced by thermal stress, make the cells more resistant to following stress. We aimed to verify if rats heating reduces the production of TNF-alpha in a model of acute respiratory distress syndrome (ARDS).


METHODS
We divided 34 Wistar rats in Group A and Group B. The rats of Group A received thermal shock and tracheal instillation of lipopolysaccharide (LPS), the rats of Group B only tracheal instillation of LPS. All the rats have been then subdivided in 6 subgroups sacrificed at different times after instillation of LPS: A30 and B30 at 30 min, A90 and B90 at 90 min and A180 and B180 at 180 min. We dosed the serum concentration of TNF-alpha and the values of every subgroup have been compared with the Student t-test.


RESULTS
The serum concentration of TNF-alpha in the subgroup A90 resulted significantly lower than in the subgroup B90.


CONCLUSIONS
The exposure to thermal stress resulting in the production of HSPs reduced the concentration of TNF-alpha in this model of ARDS.
Di Filippo
A
A
Section of Anaesthesia, Department of Critical Care, University of Florence, Viale Morgagni 85, 50124 Florence, Italy. aledanibatibeba@hotmail.com
Rinaldi
S
S
Pascente
C
C
Messeri
D
D
De Gaudio
A R
AR
D000818:Animals; D001831:Body Temperature; D004195:Disease Models, Animal; D006358:Hot Temperature; D007322:Instillation, Drug; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012128:Respiratory Distress Syndrome / Q000276:immunology; D014132:Trachea; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000187:drug effects
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha