Expression of base excision DNA repair genes as a biomarker of oxidative DNA damage.
Oxidative stress induced DNA damage is considered to be the most common insult affecting the genome. Moreover, it is recognized as a common pathway to mutations and is suggested to play a major role in the development of chronic diseases such as cancer. However, current analytical methods used to detect oxidative DNA damage have been hampered by both technical and biological obstacles. These include spurious oxidation during DNA isolation and processing, and the inherent removal of damaged bases by numerous operating DNA repair systems. The removal of oxidized bases is performed predominantly by the base excision repair (BER) pathway and it has been shown that induction of DNA repair genes occurs in response to oxidative stress. Here, we demonstrate the utility of measuring changes in expression of BER genes as a sensitive in vivo biomarker for oxidative DNA damage.
Powell
Christine L
CL
Curriculum in Toxicology and Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, NC 27599, USA.
Swenberg
James A
JA
Rusyn
Ivan
I
D015415:Biomarkers / Q000032:analysis; D020552:Comet Assay; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D045643:DNA Repair Enzymes / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
D015415:Biomarkers; D017382:Reactive Oxygen Species; D045643:DNA Repair Enzymes