[Analysis of gene expression profile of G1/S transition in gastric cancer cell cycle].
OBJECTIVE
To detect the variation of gene expression profile of G1/S transition and elucidate the role of related genes regulating cell cycle from G1 to S phase in gastric cancer.


METHODS
Nocodazole-thymidine and double thymidine methods were used to synchronize gastric cells at G2/M and G1/S point, cDNA microarray chips was applied to examine the gene expression profile at G1 early and late phase, S early and late phase during the cell cycle, hierarchy analysis was conducted by a professional software package.


RESULTS
A total of 2001 genes were detected available, 959 genes appeared to be upregulated or downregulated, including 147 genes upregulated at G1 late phase. These 147 genes are involved in DNA metabolism, transcription and translation,posttranslational modification, ubiquitination, signal transduction etc, which all affected cell cycle from different aspects.


CONCLUSIONS
Complex multiple gene processes, such as DNA metabolism, transcription and translation, posttranslational modification, ubiquitination, signal transduction etc,are implicated in and also essential for G1/S transition during gastric cancer cell cycle, part of these genes are significantly associate with overproliferation in gastric cancer.
Lan
Bin
B
Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Liu
Bing-ya
BY
Zhang
Ji
J
Wang
Kan-kan
KK
Chen
Xue-hua
XH
Zhu
Zheng-gang
ZG
D002455:Cell Division; D016193:G1 Phase / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016196:S Phase / Q000235:genetics*; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000503:physiopathology; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured