Functional Genomics meets neurodegenerative disorders Part I: transcriptomic and proteomic technology.
Transcriptomics and proteomics are increasingly applied to gain a mechanistic insight into neurodegenerative disorders. These techniques not only identify distinct, differentially expressed mRNAs and proteins but are also employed to dissect signaling pathways and reveal networks by using an integrated approach. In part I of this back-to-back review, technical aspects are discussed: in the transcriptomics section, which includes enrichment by laser microcapture dissection, we comment on qRT-PCR, SAGE, subtractive hybridization, differential display and microarrays, including software packages. In the proteomics section we discuss two-dimensional (2D) gel electrophoresis, liquid chromatography, methods to label and enrich specific proteins or peptides, and different types of mass spectrometers. These tools have been applied to a range of neurodegenerative disorders and are discussed and integrated in part II (Functional Genomics meets neurodegenerative disorders. Part II: application and data integration).
David
Della C
DC
Brain and Mind Research Institute, University of Sydney, 100 Mallett St., Camperdown, NSW 2050, Australia.
Hoerndli
Frederic
F
Götz
Jürgen
J
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods / Q000639:trends; D020869:Gene Expression Profiling / Q000379:methods* / Q000639:trends; D023281:Genomics / Q000379:methods* / Q000639:trends; D006801:Humans; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics; D019636:Neurodegenerative Diseases / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000639:trends; D040901:Proteomics / Q000379:methods* / Q000639:trends; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000639:trends
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger