Imbalance between soluble tumour necrosis factor receptors type 1 and 2 in chronic periodontitis.
BACKGROUND
Soluble types of tumour necrosis factor (TNF) receptors type 1 and 2 modulate the TNF-alpha-mediated inflammatory responses in chronic periodontitis (CP).


OBJECTIVE
This study investigated the levels of TNF-alpha, soluble TNF receptor type 1 and 2 in gingival crevicular fluid (GCF) and serum of healthy subjects and CP patients.


METHODS
Thirty-eight sera and 73 GCF samples were collected from 16 healthy subjects and 22 CP patients. GCF was collected from probing pocket depth (PPD)< or =3 mm sites of healthy subjects, PPD< or =3, 4-6 and > or =7 mm sites of CP patients. The levels of TNF-alpha, soluble TNF receptor type 1 and 2 in the serum and GCF were quantified by enzyme-linked immunosorbant assay.


RESULTS
The total amounts of TNF-alpha, soluble TNF receptor type 1 and 2 in GCF significantly elevated with increasing PPD in both site-based (p<0.05) and subject-based (p<0.05) analyses. However, their levels progressively diverged as the pocket depths increased, with the soluble TNF receptor type 2 level being comparatively lower than type 1. On the other hand, soluble TNF receptor type 2/type 1 ratios in GCF decreased as the severity of periodontitis increased (p<0.0001).


CONCLUSIONS
The imbalance between soluble TNF receptor type 1 and 2 levels in GCF could be related to CP severity.
Ikezawa
Ikuyo
I
Division of Periodontology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Tai
Hideaki
H
Shimada
Yasuko
Y
Komatsu
Yasutaka
Y
Galicia
Johnah C
JC
Yoshie
Hiromasa
H
D016022:Case-Control Studies; D002908:Chronic Disease; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000097:blood*; D018709:Statistics, Nonparametric; D047990:TNF Receptor-Associated Factor 1 / Q000032:analysis* / Q000097:blood; D047992:TNF Receptor-Associated Factor 2 / Q000032:analysis* / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D047990:TNF Receptor-Associated Factor 1; D047992:TNF Receptor-Associated Factor 2; D014409:Tumor Necrosis Factor-alpha