The effect of block copolymer structure on the internalization of polymeric micelles by human breast cancer cells.
The objective of this study was to assess the effect of hydrophilic/hydrophobic block chain lengths on the internalization of poly(ethylene oxide)-block-poly(epsilon-caprolactone) (PEO-b-PCL) micelles by cancer cells. PEO-b-PCL block copolymers with varied PEO and PCL chain lengths were synthesized, assembled to polymeric micelles and loaded with a hydrophobic fluorescent probe (DiI) through a co-solvent evaporation method of physical encapsulation. The slow release of the fluorescent probe from the micellar structure was evidenced following DiI transfer to lipid vesicles. The extent of micellar uptake by cancer cells was investigated through their incubation with MCF-7 cells followed by measurement of the fluorescent emission intensity of DiI (lambda=550 nm) in separated lysed cells. Cellular internalization of polymeric micelles was confirmed by laser scanning microscopy. The mechanism of micellar uptake was investigated by pretreatment of MCF-7 cells with chlorpromazine and cytochalasin B. Encapsulation of DiI in PEO-b-PCL micelles lowered the extent and rate of hydrophobic probe internalization by cancer cells. For polymeric micelles with 5000 gmol(-1) of PCL and varied PEO molecular weights of 2000, 5000 and 13,000 gmol(-1), maximum uptake was observed at a PEO molecular weight of 5000 gmol(-1). For polymeric micelles with 5000 gmol(-1) of PEO and varied PCL molecular weights of 5000, 13,000 and 24,000 gmol(-1), maximum uptake was observed at 13,000 gmol(-1) of PCL. Chlorpromazine reduced the cellular uptake of PEO-b-PCL micelles independent from the block copolymer structure, pointing to the involvement of clathrin mediated endocytosis mechanisms in the uptake of polymeric micelles by cancer cells. Inhibition of cellular uptake of PEO-b-PCL micelles by cytochalasin B, on the other hand, was found to be dependent on the chemical structure of the core/shell forming blocks.
Mahmud
Abdullah
A
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, 4119 DPC Edmonton, Alta. T6G 2N8 Canada.
Lavasanifar
Afsaneh
A
D001943:Breast Neoplasms / Q000737:chemistry / Q000378:metabolism*; D002232:Carbocyanines / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D003080:Cold Temperature; D016503:Drug Delivery Systems; D005260:Female; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D018613:Microscopy, Confocal; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D013194:Staining and Labeling
D002232:Carbocyanines; D005456:Fluorescent Dyes; D008823:Micelles; D011091:Polyesters; C458722:polyethylene oxide-polycaprolactone copolymer; C024286:3,3'-dioctadecylindocarbocyanine