Feasibility of using fluoresceinyl Cypridina luciferin analog in a novel chemiluminescence method for real-time photodynamic therapy dosimetry.
Singlet oxygen ((1)O(2)) is the most important cytotoxic agent in photodynamic therapy (PDT). The feasibility of using a chemiluminescence (CL) probe, 3,7-dihydro-6-[4-(2-(N'-(5-fluoresceinyl)thioureido)ethoxy)phenyl]-2-methylimidazo{1,2-a}pyrazin-3-one sodium salt (fluoresceinyl Cypridina luciferin analog, FCLA), to monitor (1)O(2) production during PDT is evaluated in vitro. Lymphoma cells were treated with various protocols of PDT. The results show that the FCLA-CL production during PDT is linearly related to the corresponding cytotoxicity, regardless of the treatment protocol. With minimum cytotoxicity and interference to the PDT treatment outcome, the FCLA-CL system is an effective means to quantify PDT (1)O(2) production and may provide an alternative real-time dosimeter.
Qin
Yanfang
Y
Institute of Laser Life Science, South China Normal University, Guangzhou, 510631, PR China.
Xing
Da
D
Luo
Shiming
S
Zhou
Jing
J
Zhong
Xueyun
X
Chen
Qun
Q
D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D017323:Dihematoporphyrin Ether / Q000737:chemistry; D005240:Feasibility Studies; D005452:Fluoresceins / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D049449:Luminescence; D010778:Photochemotherapy / Q000379:methods; D011874:Radiometry; D026082:Singlet Oxygen / Q000737:chemistry; D019810:Sodium Azide / Q000737:chemistry / Q000494:pharmacology
D005452:Fluoresceins; D007093:Imidazoles; C080213:3,7-dihydro-6-(4-(2-(N'-(5-fluoresceinyl)thioureido)ethoxy)phenyl)-2-methylimidazo-(1,2-a)pyrazin-3-one; D026082:Singlet Oxygen; D019810:Sodium Azide; D017323:Dihematoporphyrin Ether