title: Differential amplification of murine bipotent megakaryocytic/erythroid progenitor and precursor cells during recovery from acute and chronic erythroid stress.
abstract: Two murine bipotent erythroid/megakaryocytic cells, the progenitor (MEP) and precursor (PEM) cells, recently have been identified on the basis of the phenotypes of linnegc-kitposSca-1neg CD16/CD32lowCD34low and TER119pos4A5pos or 2D5pos, respectively. However, the functional relationship between these two subpopulations and their placement in the hemopoietic hierarchy is incompletely understood. We compared the biological properties of these subpopulations in marrow and spleen of mice with and without acute or chronic erythroid stress. MEP cells, but not PEM cells, express c-kit, respond to stem cell factor in vitro, and form spleen colonies in vivo. PEM cells comprise up to 50%-70% of the cells in BFU-E-derived colonies but are not present among the progeny of purified MEP cells cultured under erythroid and megakaryocytic permissive conditions. PEM cells increase 10- to 20-fold under acute and chronic stress, whereas MEP cell increases (21%-84%) are observed only in acutely stressed animals. These data suggest that MEP and PEM cells represent distinct cell populations that may exist in an upstream-downstream differentiation relationship under conditions of stress. Whereas the dynamics of both populations are altered by stress induction, the differential response to acute and chronic stress suggests different regulatory mechanisms. A model describing the relationship between MEP, PEM, and common myeloid progenitor cells is presented.
authors.affiliation: Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Sanchez
authors.forename: Irving L
authors.initials: IL
authors.lastname: Weissman
authors.forename: Maria
authors.initials: M
authors.lastname: Pallavicini
authors.forename: Mauro
authors.initials: M
authors.lastname: Valeri
authors.forename: Paola
authors.initials: P
authors.lastname: Guglielmelli
authors.forename: Alessandro Maria
authors.initials: AM
authors.lastname: Vannucchi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Migliaccio
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Migliaccio
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001853:Bone Marrow / Q000378:metabolism*; D002454:Cell Differentiation; D015672:Erythroid Precursor Cells / Q000378:metabolism / Q000502:physiology*; D004920:Erythropoiesis; D005434:Flow Cytometry; D050982:GATA1 Transcription Factor / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000502:physiology*; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D010659:Phenylhydrazines / Q000494:pharmacology; D013154:Spleen / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D050982:GATA1 Transcription Factor; D010659:Phenylhydrazines; C030299:phenylhydrazine