title: Isolation of alpha- and beta-subunits of peak-I hCG and generation of highly specific polyclonal antisera.
abstract: Development of polyclonal antisera is still a choice in some hard-pressed budget laboratories. In the present study, an attempt was made to isolate alpha- and beta-subunits from peak-I hCG, generation of polyclonal antisera and their characterization. The anti-hCG-a antisera showed titres of 1: 8000 and anti-hCG-beta antisera 1: 16,000 at 50% binding to radiolabelled hCG in RIA. Studies on specificity using anti hCG-beta antiserum demonstrated no cross-reaction with several hormones tested in the present study, except for hCG-beta and hCG, thus eliciting a highly specific hCG-beta antiserum.
authors.affiliation: Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, Munirka, New Delhi, India.
authors.forename: Pramod V
authors.initials: PV
authors.lastname: Prasad
authors.forename: Shail K
authors.initials: SK
authors.lastname: Chaube
authors.forename: T G
authors.initials: TG
authors.lastname: Shrivastav
authors.forename: G L
authors.initials: GL
authors.lastname: Kumari
authors.forename: S
authors.initials: S
authors.lastname: Duraiswami
authors.forename: K
authors.initials: K
authors.lastname: Muralidhar
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000276:immunology* / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D015292:Glycoprotein Hormones, alpha Subunit / Q000276:immunology / Q000302:isolation & purification*; D007106:Immune Sera; D011817:Rabbits
chemical_list: D018997:Chorionic Gonadotropin, beta Subunit, Human; D015292:Glycoprotein Hormones, alpha Subunit; D007106:Immune Sera