Cell death and cell proliferation in mouse submandibular gland during early post-irradiation phase.
The effects of irradiation on different cell compartments in the submandibular gland were analyzed in adult C57BL/6 mice exposed to X-ray irradiation and followed up for 10 days. Apoptosis was quantified using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end labeling method (TUNEL). Cell proliferation was detected using immunohistochemistry for proliferating cell nuclear antigen (PCNA). Radiation-induced apoptosis occurred rapidly, reaching a maximum 3 days post-irradiation. The percentage of apoptotic cells increased with the irradiation dose. At day 1 post-irradiation, cell proliferation was significantly reduced in comparison to sham-irradiated controls. After post-irradiation arrest of the cell cycle, proliferation increased in all gland compartments, reaching a maximum at day 6 post-irradiation. The proliferation response corresponded to the dose of irradiation. We suggest that the reason for gland dysfunction could be the coexistence of high apoptotic and proliferative activity in the irradiated gland.
Bralic
Marina
M
Department of Histology and Embryology, Faculty of Medicine, University of Rijeka, Croatia. bralic_marina@yahoo.com
Muhvic-Urek
Miranda
M
Stemberga
Valter
V
Golemac
Mijo
M
Jurkovic
Slaven
S
Borcic
Josipa
J
Braut
Alen
A
Tomac
Jelena
J
D000818:Animals; D001835:Body Weight; D002453:Cell Cycle / Q000528:radiation effects; D016923:Cell Death / Q000528:radiation effects*; D049109:Cell Proliferation / Q000528:radiation effects*; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D013363:Submandibular Gland / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D014965:X-Rays