title: Development of a new colorimetric method determining the yield of microencapsulation of alpha-tocopherol.
abstract: Microencapsulation of alpha-tocopherol effectively protects alpha-tocopherol from oxidation and produces high-value-added and long-shelf-stable foods. High-performance liquid chromatography (HPLC) has been applied to measure the yield of microencapsulated alpha-tocopherol with high accuracy; however, it takes long analysis time. An alternative method is required to determine the yield of microencapsulated alpha-tocopherol in food industry. A new, easy, and sensitive colorimetric method using 5% cupric acetate pyridine and oleic acid was developed. Correlation coefficient (r) of colorimetric method on alpha-tocopherol in microencapsulation system and of results between colorimetric method and HPLC were +0.996 and +0.989, respectively, which indicates that this novel colorimetric method can be successfully applied to evaluate the yield of microencapsulated alpha-tocopherol instead of HPLC. The optimum storage temperature and pH of microencapsulated alpha-tocopherol for 7-day storage were 25 degrees C and pH 9, respectively, determined by this new colorimetric method.
authors.affiliation: Department of Food Science and Technology, Seoul National University of Technology, Korea. pschang@snut.ac.kr
authors.forename: Pahn-Shick
authors.initials: PS
authors.lastname: Chang
authors.forename: Jonghyuk
authors.initials: J
authors.lastname: Lee
authors.forename: Jaehwan
authors.initials: J
authors.lastname: Lee
mesh_terms: D002214:Capsules / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry / Q000379:methods*; D004339:Drug Compounding; D012680:Sensitivity and Specificity; D024502:alpha-Tocopherol / Q000032:analysis*
chemical_list: D002214:Capsules; D024502:alpha-Tocopherol