Determination of active components in Cynanchum chinense R. Br. by capillary electrophoresis.
A method for the determination of 7-O-alpha-l-rhamnopyranosyl-kaempferol-3-O-beta-d-glucopyranoside (GL) and 7-O-alpha-l-rhamnopyranosyl-kaempferol-3-O-alpha-l-rhamnopyranoside (RH) in the traditional Chinese herb Cynanchum chinense R. Br. by capillary electrophoresis has been developed. With botate buffer (30 mmol/L, pH 9.50) as running buffer and an applied voltage of 20 kV, the compounds were completely separated within 6 min and detected at UV 254 nm. The correlation coefficients of the calibration curves for GL and RH were 0.9990 and 0.9992, respectively, over the concentration ranges (15.0-1000.0 and 12.0-1000.0 microg/mL), and the recoveries were from 91.4 to 107.1%.
Liu
Huitao
H
Department of Applied Chemistry, Yantai University, Yantai 264005, People's Republic of China. liuht@lzu.edu.cn
Gao
Yuan
Y
Wang
Ketai
K
Hu
Zhide
Z
D031105:Cynanchum / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D044949:Kaempferols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results
C512122:7-O-alpha-L-rhamnopyranosyl-kaempferol-3-O-alpha-L-rhamnopyranoside; C512123:7-O-alpha-L-rhamnopyranosyl-kaempferol-3-O-beta-D-glucopyranoside; D005960:Glucosides; D006027:Glycosides; D044949:Kaempferols