Comparison of established cell lines at different passages by karyotype and comparative genomic hybridization.
Two established cancer cell lines, MCF-7 and Ishikawa, were both obtained directly from a cell repository and through another laboratory. The karyotypes from the two MCF-7 cell lines had up to 83 chromosomes and similarities for chromosomal gain and structural abnormalities. The two Ishikawa cell lines had up to 60 chromosomes with only a missing X as the common chromosome abnormality. CGH studies were performed by co-hybridizing the two Ishikawa or MCF-7 cell lines to normal metaphases. The differences seen between the two MCF-7 cell cultures reflect changes due to passage number and culture conditions. For Ishikawa, DNA polymorphic data and mutation studies suggest that the two cell lines are not derived from the same established tumor cell line. Our study shows the utilization of CGH in comparing cell lines originating from the same specimen. Our study also demonstrates the necessity for periodically evaluating cell lines to confirm their origin.
Wenger
Sharon L
SL
Department of Pathology, West Virginia University, P. O. Box 9203, Morgantown, WV, 26506-9203, USA. swenger@hsc.wvu.edu
Senft
Jamie R
JR
Sargent
Linda M
LM
Bamezai
Ramesh
R
Bairwa
Narendra
N
Grant
Stephen G
SG
D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D045744:Cell Line, Tumor / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D016889:Endometrial Neoplasms / Q000235:genetics; D005260:Female; D023281:Genomics; D006801:Humans; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D016324:Sequence Tagged Sites
D017931:DNA Primers; D004273:DNA, Neoplasm