[Genomic changes in primary lesion and lymph node metastases of esophageal squamous cell carcinoma].
OBJECTIVE
Local lymph node and blood metastasis could occur at early stage of esophageal squamous cell carcinoma (ESCC), which may be the key factors of its recurrence and poor prognosis. However, the mechanism of ESCC metastasis is unclear. This study was to analyze the genetic changes in primary lesion and lymph node metastases of ESCC, to screen for and locate ESCC metastasis-related genes.


METHODS
Genomic alterations in 15 pairs of primary lesions and matched metastatic lymph nodes of ESCC were analyzed by comparative genomic hybridization (CGH).


RESULTS
In the 15 pairs of tissues, the most common chromosomal alterations were the gains of 3q, 8q, 6p, 20p, 5p, 18p, 2p, 2q and 1q, and the losses of 10p, 10q, 17p, 18q, 4p and 13q. Of these changes, the most significant finding was the gain of 6p with a frequency of 47% in metastatic lymph nodes and 13% in primary lesions, and the gain of 20p with a frequency of 73% in metastatic lymph nodes and 33% in primary lesions. The second interesting finding was the loss of 10p with a frequency of 53% in metastatic lymph nodes and 13% in primary lesions, and the loss of 10q with a frequency of 47% in metastatic lymph nodes and 13% in primary lesions.


CONCLUSIONS
The gains of 6p and 20p and the losses of 10p and 10q are common genomic alterations in primary lesion and lymph node metastases of ESCC, which may code ESCC metastasis-related genes.
Qin
Yan-Ru
YR
Department of Oncology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, P.R. China.
Wang
Li-Dong
LD
Dora
Kwong
K
Guan
Xin-Yuan
XY
Zhuang
Ze-hao
ZH
Fan
Zong-Min
ZM
Deng
Wen
W
Cao
Shi-Hua
SH
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002872:Chromosome Deletion; D002879:Chromosomes, Human, Pair 10; D002890:Chromosomes, Human, Pair 20; D002896:Chromosomes, Human, Pair 6; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods