The -250G-->A polymorphism in the human hepatic lipase gene promoter affects blood lipids in Chinese.
BACKGROUND
Human hepatic lipase (HL) is a glycoprotein that catalyzes the hydrolysis of triglycerides and phospholipids in all major classes of lipoproteins. We studied whether the hepatic lipase gene -250G(guanine)-->A(adenine) polymorphism affect blood lipids level and the coronary heart disease.


METHODS
Two hundred and thirty subjects were included. Among them there were 122 patients with coronary heart disease and 108 subjects without coronary heart disease. Polymerase chain reaction-restricted fragments length polymorphism was used to determine HL genotype.


RESULTS
The serum HDL-C level of HL-250A heterozygote (carriers of GA genotype) and homozygote (carriers of AA genotype) [(1.32+/-0.35) mmol/l] was significantly higher than wild type [carriers of GG genotype, (1.19+/-0.30) mmol/l, P<0.005]. This effect to blood lipids appears more evident in women (P<0.005). But the distribution of the 3 genotypes of HL-250 among the patients with coronary heart disease (GG54.1%, GA37.7%, AA8.2%) were similar with those of the control (GG54.6%, GA37.0%, AA8.4%, P>0.05). Both the male and the female had similar ratio for 3 HL genotypes.


CONCLUSIONS
HL-250G-->A variation affects blood lipids profile and results in the increasing of the serum HDL-C level. This beneficial effect to blood lipids profile is more obviously seen in the female.
Zhao
Shuiping
S
Department of Cardiology, The Second Xiangya Hospital, Central South University, 86# Renmin Middle Road, Changsha, Hunan 410011, PR China.
Xie
Xiangzhu
X
Nie
Sai
S
D000368:Aged; D001483:Base Sequence; D016022:Case-Control Studies; D002681:China; D003327:Coronary Disease / Q000097:blood / Q000235:genetics*; D017931:DNA Primers; D006801:Humans; D008049:Lipase / Q000235:genetics*; D008055:Lipids / Q000097:blood*; D008099:Liver / Q000201:enzymology*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
D017931:DNA Primers; D008055:Lipids; D008049:Lipase