Perspectives for establishment and reorganization of carbohydrate-directed CD antibodies. Report of the carbohydrate section.
Complex glycosylated glycoproteins, glycolipids and proteoglycans are expressed on the cell surface and are also found as constituents of the extracellular matrix (ECM). Interactions of the carbohydrate moiety of these macromolecules with specific receptors (lectins) are involved in many functions of immune cells such as cell-cell or cell-ECM adhesion, recognition, and neutralization of pathogens and regulation of apoptosis. For studies on live cells mAbs recognizing distinct oligosaccharide structures are useful tools because in contrast to other analytical methods of carbohydrate biochemistry they are able to react with glycans in the complex sterical context of the cell surface. In general expression patterns of carbohydrate mAbs depend on (i) the number and type of carriers to which the glycans are linked (glycoproteins, glycolipids), (ii) the steric situation on the cell surface, and (iii) modifications of the basic glycotope (different branching, chain length, masking by sialylation, sulphation or fucosylation).
Schwartz-Albiez
Reinhard
R
German Cancer Research Center, Heidelberg, Germany. r.s-albiez@dkfz.de
Kniep
Bernhard
B
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D015703:Antigens, CD / Q000737:chemistry / Q000276:immunology*; D002241:Carbohydrates / Q000276:immunology*; D002460:Cell Line; D006801:Humans
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D002241:Carbohydrates