Cloning and expression studies of the Dunaliella salina UDP-glucose dehydrogenase cDNA.
The enzyme UDP-glucose dehydrogenase (EC 1.1.1.22) converts UDP-glucose to UDP-glucuronate. Plant UDP-glucose dehydrogenase (UGDH) is an important enzyme in the formation of hemicellulose and pectin, the components of primary cell walls. A cDNA, named DsUGDH, (GeneBank accession number: AY795899) corresponding to UGDH was cloned by RT-PCR approach from Dunaliella salina. The cDNA is 1941-bp long and has an open reading frame encoded a protein of 483 amino acids with a calculated molecular weight of 53 kDa. The derived amino acids sequence shows high homology with reported plants UGDHs, and has highly conserved amino acids motifs believed to be NAD binding site and catalytic site. Although UDP-glucose dehydrogenase is a comparatively well characterized enzyme, the cloning and characterization of the green alga Dunaliella salina UDP-glucose dehydrogenase gene is very important to understand the salt tolerance mechanism of Dunaliella salina. Northern analyses indicate that NaCl can induce the expression the DsUGDH.
Qinghua
He
H
Key Laboratory of bio-resources and Eco-environment (Sichuan University), Ministry of Education, Chengdu, China.
Dairong
Qiao
Q
Qinglian
Zhang
Z
Shunji
He
H
Yin
Li
L
Linhan
Bai
B
Zhirong
Yang
Y
Yi
Cao
C
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D001665:Binding Sites; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D056890:Eukaryota / Q000201:enzymology*; D008956:Models, Chemical; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology; D014018:Tissue Distribution; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000737:chemistry* / Q000235:genetics*
D000596:Amino Acids; D018076:DNA, Complementary; D012965:Sodium Chloride; D014533:Uridine Diphosphate Glucose Dehydrogenase