The role of Rac1 and Rac2 in bacterial killing.
The small Rho guanosine triphosphatases (GTPases) Rac1 and Rac2 have distinct roles in regulating neutrophil chemotaxis; however, little is known about their possible unique roles in mediating bacterial killing. To elucidate the relative roles of Rac1 and Rac2 in regulating neutrophil-mediated bacterial killing, we utilized the previously described mice model in which mouse neutrophils are deficient in either Rac1, Rac2, or both isoforms. We demonstrate here that while both Rac isoforms are required for normal neutrophil chemotaxis and bacterial killing, they have non-overlapping roles in bacterial phagocytosis and NADPH oxidase function.
Koh
Adeline Loo Yee
AL
Faculty of Dentistry, University of Toronto, Toronto, Canada.
Sun
Chun Xiang
CX
Zhu
Fei
F
Glogauer
Michael
M
D000818:Animals; D002634:Chemotaxis, Leukocyte; D004926:Escherichia coli / Q000166:cytology / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D009195:Peroxidase / Q000378:metabolism; D010587:Phagocytosis; D020033:Protein Isoforms / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D020830:rac1 GTP-Binding Protein / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000097766:RAC2 GTP-Binding Protein
D009195:Peroxidase; D020033:Protein Isoforms; D020744:rac GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein; D000097766:RAC2 GTP-Binding Protein