Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain reaction-denaturing gradient gel electrophoresis assay.
To investigate the microbial communities of microorganisms cultivated under different carbon sources, three sequencing batch reactors were operated. They were supplied with sewage, glucose and sodium acetate as carbon sources respectively and showed high phosphorus removal performance. The results of denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction-amplified (PCR) 16S rDNA fragments demonstrated that beta-protebacteria, Actinomyces sp. and gamma-protebacteria only exited in 1 # reactor. The microbiological diversity of 1 # reactor exceeded the other two reactors. Flavobacterium, Bacillales, Actinomyces, Actinobacteridae and uncultured bacteria (AF527584, AF502204, AY592749, AB076862, AJ619051, AF495454 and AY133070) could be detected in the biological phosphorus removal reactors.
Yu
Shui-Li
SL
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
Liu
Ya-Nan
YN
Jing
Guo-Lin
GL
Zhao
Bing-Jie
BJ
Guo
Si-Yuan
SY
D001419:Bacteria / Q000378:metabolism*; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism*; D017931:DNA Primers; D004586:Electrophoresis / Q000379:methods*; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D010710:Phosphates; D002244:Carbon