Upregulation of carp GDNF mRNA by the immunomodulator AS101.
Although the glia derived neurotrophic factor (GDNF) is defined as a molecule that maintains neuronal cells, it possesses a range of functions outside the nervous system. For example, it is essential for uretric branching in kidney morphogenesis and for regulating the differentiation of stem cells during spermatogenesis, cardiac, hair follicle and vascular differentiation and the maintenance of immune cells. In the present work, the presence of GDNF in carp peripheral blood leukocytes (PBL) and head kidney cells (HK) was evidenced and its evolutionary importance in both neural and immune systems development was suggested. Using the northern-blot technique, we could observe the expression of two different transcripts of this gene. GDNF upregulation was detected using semi-quantitative PCR, following ex vivo treatment of PBL and HK cells with the immunomodulator AS101 which was previously shown to inhibit IL-10 and to up-regulate GDNF protein levels in human SVG astrocyte cell line, in 6-OHDA hemi-parkinsonian mice in vivo and in rat glomerular mesengial cells in vitro.
Okun
Eitan
E
Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, 5600 Nathan, Shock Drive, Baltimore, MD 21224, USA. redgingi@yahoo.com
Saida
Hagar
H
Albeck
Michael
M
Sredni
Benjamin
B
Avtalion
Ramy R
RR
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002347:Carps / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005030:Ethylenes / Q000494:pharmacology*; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000096:biosynthesis*; D007155:Immunologic Factors / Q000494:pharmacology*; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
D005030:Ethylenes; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007155:Immunologic Factors; D012333:RNA, Messenger; D016753:Interleukin-10; C054105:ammonium trichloro(dioxoethylene-O,O'-)tellurate