Analysis of public single nucleotide polymorphisms in commercial pig populations.
More than 5500 pig single nucleotide polymorphisms (SNPs) were recently identified and deposited in the public domain. To test the usefulness of these public SNPs, 109 SNPs were analysed for polymorphism within six commercial pig populations. A functional polymerase chain reaction (PCR) assay was obtained for 103 SNPs and it was possible to validate c. 59% by PCR-restriction fragment length polymorphism. Furthermore, polymorphism was found using a relatively limited number of genomic DNA samples, indicating that these polymorphisms are segregating at a useful frequency in these populations. The high percentage of validated markers demonstrates the utility of these public pig SNPs to identify loci responsible for economically important traits in commercial pig populations.
Kollers
S
S
Sygen International plc, Sygen Cambridge Research Laboratory, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
Mégy
K
K
Rocha
D
D
D000818:Animals; D017931:DNA Primers; D030541:Databases, Genetic; D014644:Genetic Variation; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D034421:Sus scrofa / Q000235:genetics*
D017931:DNA Primers