Fluorescent properties of spinach leaf plasma membranes and chloroplast envelopes.
Plasma membranes and chloroplast envelopes were isolated from green spinach leaves, and emission and excitation spectra recorded in aqueous solution at 25 degrees C. Chloroplast envelopes excited by 420 nm showed strong emission peaks at 635 and 680 nm that came from chlorophyll precursors present only in these membranes. Upon UV excitation, both plasma membranes and chloroplast envelopes exhibited emission peak at 420 nm originating from pterins and 520 nm due to flavins. Oxidation of the membranes increased both the emission and excitation fluorescence intensity.
Cvetić
Tijana
T
Faculty of Biology, University of Belgrade, Serbia and Montenegro. cvetic@ibiss.bg.ac.yu
Vucinić
Zeljko
Z
D002462:Cell Membrane / Q000737:chemistry*; D002736:Chloroplasts / Q000737:chemistry*; D005453:Fluorescence; D010788:Photosynthesis; D018515:Plant Leaves / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D018724:Spinacia oleracea / Q000737:chemistry*