Single short-term signal that enhances cooperative activity of old rat hepatocytes acts for several days.
Primary cultures of rat hepatocytes were studied in serum-free medium. Ultradian protein synthesis rhythm was used as a marker of overall cell synchronization and cooperation amongst the population. The level of synchronization was determined by amplitudes of the rhythm. Low synchronization of old rat hepatocytes can be enhanced by addition of either gangliosides or phenylephrine to the medium. Incubation of cultures with gangliosides lasted for 2.5 h, while action of phenylephrine was only for 2 min. The amplitude of protein synthesis rhythm was increased 1.5-2 times. In cultures transferred to a fresh normal medium, this increased amplitude was observed for at least 2-3 days. Thus, both gangliosides and phenyleprine are triggers, which, as shown earlier, initiated calcium-dependent processes in the cytoplasm. The results are discussed in the light of concept of the cell self-organization by a direct cell-cell communication.
Brodsky
Vsevolod
V
Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov Street, 117808 Moscow, Russia. brodsky@proxima.idb.ac.ru
Zvezdina
Natalia D
ND
Nechaeva
Natalia V
NV
Novikova
Tatjana E
TE
Gvasava
Inessa G
IG
Fateeva
Valintina I
VI
Malchenko
Ludmila A
LA
D000818:Animals; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002450:Cell Communication; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology*; D007477:Ions; D008297:Male; D010656:Phenylephrine / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013997:Time Factors
D003470:Culture Media; D016895:Culture Media, Serum-Free; D005732:Gangliosides; D007477:Ions; D010656:Phenylephrine; D002118:Calcium