Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism.
The enantioselectivity of a Burkholderia cepacia lipase toward secondary alcohols could be both increased and decreased rationally by introducing only a single mutation on the basis of the mechanism proposed previously.
Ema
Tadashi
T
Department of Applied Chemistry, Faculty of Engineering, Okayama University, Tsushima, Okayama 700-8530, Japan. ema@cc.okayama-u.ac.jp
Fujii
Toshiyuki
T
Ozaki
Misa
M
Korenaga
Toshinobu
T
Sakai
Takashi
T
D000438:Alcohols / Q000737:chemistry; D016956:Burkholderia cepacia / Q000201:enzymology; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008049:Lipase / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013237:Stereoisomerism
D000438:Alcohols; D004269:DNA, Bacterial; D008049:Lipase