Inhibition of superoxide dismutase, Vitamin C and glutathione on chemiluminescence produced by luminol and the mixture of sulfite and bisulfite.
In a system which consisted of luminol (3-aminophthalhydrazide), cobalt sulfate (CoSO4), alkaline buffer and the mixture of NaSO3 and sodium bisulfite (NaHSO3) (sulfite and bisulfite=3:1, m/m), a strong chemiluminescence (CL) was observed using a BPCL ultra-weak luminometer. The CL signals resulted from 3-aminophthalate (the product of oxidized luminol), and were affected by the buffer pH, buffer medium and the concentrations of luminol, CoSO4 and the NaSO3-NaHSO3 mixture. The observation that the CL intensities were inhibited by superoxide dismutase (SOD), Vitamin C (Vc) and glutathione (GSH) in a dose-dependent manner suggested that superoxide radical (O2*-) was involved in the CL reaction and responsible for oxidation of luminol.
Geng
Hong
H
Institute of Environmental Medicine and Toxicology, Shanxi University, Wucheng Road, Taiyuan 030006, PR China.
Meng
Ziqiang
Z
D001205:Ascorbic Acid / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D049449:Luminescence; D008165:Luminol / Q000737:chemistry*; D013447:Sulfites / Q000737:chemistry; D013482:Superoxide Dismutase / Q000737:chemistry*
D013447:Sulfites; D003035:Cobalt; D008165:Luminol; D013482:Superoxide Dismutase; D005978:Glutathione; C026305:cobalt sulfate; D001205:Ascorbic Acid; C009279:sodium bisulfite; C025026:sodium sulfite