Validation assay for total flavonoids, as rutin equivalents, from Trichilia catigua Adr. Juss (Meliaceae) and Ptychopetalum olacoides Bentham (Olacaceae) commercial extract.
An ultraviolet spectrophotometric method was validated for total flavonoid quantitation, as rutin equivalents, present in the Trichilia catigua Adr. Juss (Meliaceae) and Ptychopetalum olacoides Bentham (Olacaceae) commercial extract. Parameters as linearity, interval (range), specificity, estimated limit of detection (LOD, microg/mL), estimated limit of quantitation (LOQ, microg/mL), recovery (R, %), precision or relative standard deviation (RSD, %), and accuracy (E, %) were established. The analytical method was validated according to the experimental results: correlation coefficient (r = 0.9997); interval (RSD = 0.15-0.47%; E = 98.98-101.24%); specificity to total flavonoids quantitation, as rutin equivalents, at wavelength 361.0 nm; LOD = 0.09 microg/mL and LOQ = 0.27 microg/mL; R = 99.36-102.14%; adequate intra- and interrun precision (0.30-0.49% and 0.31-0.81%), and intra- and interrun accuracy (100.60-102.38% and 98.58-100.38%).
Rolim
André
A
University of São Paulo, Department of Pharmacy, Faculty of Pharmaceutical Sciences, 580 Prof. Lineu Prestes Ave, B1. 13, Conjunto das Químicas, 05508-900, São Paulo, Brazil. andrerb@usp.br
Maciel
Carolina P M
CP
Kaneko
Telma M
TM
Consiglieri
Vladi O
VO
Salgado-Santos
Idalina M N
IM
Velasco
Maria Valéria R
MV
D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D029582:Meliaceae / Q000378:metabolism*; D008956:Models, Chemical; D031656:Olacaceae / Q000378:metabolism*; D010936:Plant Extracts / Q000032:analysis*; D015203:Reproducibility of Results; D012431:Rutin / Q000031:analogs & derivatives / Q000032:analysis; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D014466:Ultraviolet Rays
D005419:Flavonoids; D010936:Plant Extracts; C056067:rutin sulfate; D012431:Rutin