title: BIOLAB, EPU and EMCS for cell culture experiments on the ISS.
abstract: Two ESA facilities are under development for biological research on the International Space Station: BIOLAB as part of the European "Columbus" Laboratory and the European Modular Cultivation System (EMCS), foreseen for accommodation in the US Lab "Destiny". Both facilities have an incubator (18-40 degrees C) and use standard Experiment Containers, mounted on two centrifuge rotors providing either microgravity or variable g-levels from 0.001 x g to 2.0 x g. Standard interface plates supply each container with power and data lines, with gas (controlled CO2, O2 and water vapour concentration; trace gas removal), and--for EMCS only--with water. The degree of automation is higher in BIOLAB: it contains a robotic Handling Mechanism for automatic sampling and handling of liquids, which can be stored at cool or cold temperatures or injected for automatic on-board analysis into a microscope or a spectrophotometer. For analyses on the running centrifuge, small automatic microscopes can be installed in the Experiment Containers. Several designs for supporting cell culture experiments have been studied for BIOLAB and EMCS. BIOLAB has in addition a Bio-Glovebox, which can be sterilised and where new cell cultures may be prepared under 1 x g conditions from deep-frozen samples in the Experiment Preparation Unit (EPU): the cryo-protectant will be removed by automatic washing cycles. Both facilities, EMCS and BIOLAB (with EPU), have also provisions for telescience operations through video, data and command lines, either operated by the crew or by the experimenter on ground.
authors.affiliation: European Space Agency (ESA), ESTEC/HME-GFB, Noordwijk, The Netherlands. Enno.Brinckmann@esa.int
authors.forename: Enno
authors.initials: E
authors.lastname: Brinckmann
mesh_terms: D001331:Automation; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002478:Cells, Cultured; D002498:Centrifugation; D004867:Equipment Design; D005060:Europe; D018471:Hypergravity; D007185:Incubators; D007390:International Agencies; D008853:Microscopy / Q000295:instrumentation; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D012371:Robotics; D013026:Space Flight / Q000295:instrumentation*; D013685:Telecommunications; D014893:Weightlessness