Ultrastructural effects of acute organophosphate poisoning on rat kidney.
We investigated the ultrastructural effects of the organophosphate compound methamidophos and treatment with atropine and pralidoxime (2-PAM) on rat kidneys. Male Wistar albino rats were assigned to four groups. Group 1 received 30 mg/kg methamidophos, the LD50 for this compound in rats, via oral gavage. Group 2 received only physiologic saline. Group 3 rats received 30 mg/kg methamidophos and were treated with 2-PAM and atropine via intraperitoneal injection when cholinergic symptoms were noted. Group 4 served as a control, and received physiologic saline in equivalent volumes and routes to Group 3. Kidney tissues were prepared for electron microscopic studies. No ultrastructural changes were detected in Group 1 after acute poisoning with methamidophos and in Group 3 treated with antidotes after poisoning. Acute organophosphate poisoning and antidotal treatment in this model are not associated with histopathological changes in the rat kidney but the models with different organophosphate compounds, by administrating the different dosages, may be more illuminative in explaining the effects of these chemicals in kidney.
Satar
Salim
S
Department of Emergency Medicine, School of Medicine, Cukurova University, Balcali, Adana 01330, Turkey. ssatar@cu.edu.tr
Satar
Deniz
D
Mete
Ufuk Ozgu
UO
Suchard
Jeffrey R
JR
Topal
Metin
M
Kaya
Mehmet
M
D000208:Acute Disease; D000818:Animals; D000931:Antidotes / Q000494:pharmacology; D004195:Disease Models, Animal; D007668:Kidney / Q000187:drug effects / Q000648:ultrastructure*; D007674:Kidney Diseases / Q000188:drug therapy / Q000473:pathology; D007677:Kidney Function Tests; D008297:Male; D009946:Organothiophosphorus Compounds / Q000494:pharmacology / Q000506:poisoning*; D011220:Pralidoxime Compounds / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values
D000931:Antidotes; D009946:Organothiophosphorus Compounds; D011220:Pralidoxime Compounds; C014655:methamidophos; C028797:pralidoxime