Chromatographic behavior of open-chain polyamines NH2-(CH2)2-[NH-(CH2)2]n-NH2 and their quantitative determination in sea water by high-performance ion-exchange chromatography.
The results are reported of a study using high-performance liquid chromatography for the chromatographic behavior of a homologous series of open-chain polyamines having the general molecular formula NH2-(CH2)2-[NH-(CH2)2]n-NH2 (n = 0-4). The exchange column is a carboxylate-functionalized resin. Amines are eluted with mixtures of HClO4 0.200 mol/L, NaClO4 from 0.100 to 1.40 mol/L, and acetonitrile (ACN) from 0% to 40% (v/v) and detected amperometrically. The dependence of the retention factor k' on sodium and ACN concentrations in the eluent and the total number of nitrogen atoms in the amines are considered and discussed. Suitable relationships are derived. Examples of polyamines separation are given, and a quantitative determination of analytes in spiked sea water is shown.
Crea
Francesco
F
Dipartimento di Chimica Inorganica, Chimica Analitica e Chimica Fisica, Università di Messina, Salita Sperone, 31 - I-98166 Messina (Vill. S. Agata), Italy.
De Robertis
Alessandro
A
Sammartano
Silvio
S