title: Analysis of cellular events using CellCard System in cell-based high-content multiplexed assays.
abstract: High-content screening technologies utilize assays that monitor and quantify multiple cellular events. These assays are typically performed on a single cell type with automated microscopy and image analysis. However, in order to better understand the selectivity of a compound across multiple cell lines, these types of assay must be run serially, which is time consuming. The CellCard System developed by Vitra Bioscience enables multiple cell types to be assayed within a single microtiter well, thereby enabling the simultaneous determination of cellular responses across ten cell types. This multiplexed approach could address the demand for assay capacity, increase the quality of the biologic data, reduce timelines, and improve cost-effectiveness in hit identification and lead evaluation. The authors have carried out an in-depth evaluation of this technology platform using ten cancer cell lines and a library of compounds that affect cellular growth through different mechanisms. Multiple assays were used to investigate the compound effects on membrane integrity, cell cycle progression and apoptosis. In this technology review, the authors discuss personal experience with assay validation, data analysis, results such as cell type-specific compound effects, and the potential application of the CellCard System in drug discovery.
authors.affiliation: Bristol-Myers Squibb Company, Lead Discovery & Profiling, 5 Research Parkway, Wallingford, CT 06492, USA. taosheng.chen@bms.com
authors.forename: Taosheng
authors.initials: T
authors.lastname: Chen
authors.forename: George
authors.initials: G
authors.lastname: Hansen
authors.forename: Oren
authors.initials: O
authors.lastname: Beske
authors.forename: Kristin
authors.initials: K
authors.lastname: Yates
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Zhu
authors.forename: Monique
authors.initials: M
authors.lastname: Anthony
authors.forename: Michele
authors.initials: M
authors.lastname: Agler
authors.forename: Martyn
authors.initials: M
authors.lastname: Banks
mesh_terms: D001681:Biological Assay / Q000379:methods*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002468:Cell Physiological Phenomena; D002470:Cell Survival; D006801:Humans
chemical_list: D053148:Caspase 3