Transcriptional factor FOXL2 interacts with DP103 and induces apoptosis.
Blepharophimosis-ptosis-epicanthus inversus syndrome type I is an autosomal disorder caused by mutations in FOXL2 gene and associated with premature ovarian failure in women by a dominant inheritance. FOXL2 is a recently identified protein that belongs to forkhead family transcription factor, of which signaling pathways are still unknown. Here, we show that FOXL2 induces apoptosis in both Chinese hamster ovary cells and rat granulosa cells, and it interacts with DP103, a DEAD box-containing protein. Overexpression of DP103 itself did not affect cell viability while its coexpression with FOXL2 led to the potentiation of cell death. Our results present previously undiscovered functions of these proteins, an apoptotic activity of FOXL2 in the ovary and a modulating activity of DP103 by interacting with FOXL2.
Lee
Kangseok
K
Department of Life Science, College of Natural Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Pisarska
Margareta D
MD
Ko
Jeong-Jae
JJ
Kang
Yeongseup
Y
Yoon
Seongmin
S
Ryou
Sang-Mi
SM
Cha
Kwang-Yul
KY
Bae
Jeehyeon
J
D000818:Animals; D017209:Apoptosis; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D055541:DEAD Box Protein 20; D053487:DEAD-box RNA Helicases; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors; D006801:Humans; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D020365:RNA Helicases / Q000378:metabolism*; D051381:Rats; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D004268:DNA-Binding Proteins; C421604:FOXL2 protein, human; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors; D014157:Transcription Factors; D055541:DEAD Box Protein 20; C504972:Ddx20 protein, rat; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases