title: Synthesis of the isotope-labeled derivatization reagent for carboxylic acids, 7-(N,N-dimethylaminosulfonyl)-4-(aminoethyl)piperazino-2,1,3-benzoxadiazole (d6) [DBD-PZ-NH2 (D)], and its application to the quantification and the determination of relative amount of fatty acids in rat plasma samples by high-performance liquid chromatography/mass spectrometry.
abstract: The isotope-labeled benzofurazan derivatization reagent for carboxylic acids, 7-(N,N-dimethylaminosulfonyl)-4-(aminoethyl)piperazino-2,1,3-benzoxadiazole (d6) [DBD-PZ-NH2 (D)] was synthesized. DBD-PZ-NH2 (D) was used for the accurate quantification of fatty acids by liquid chromatography/mass spectrometry (LC/MS). The standard fatty acids were derivatized with DBD-PZ-NH2 (D) to the stable isotope-labeled compounds for the fatty acids derivatives of DBD-PZ-NH2 and used for the internal standards. The obtained calibration curves for fatty acids were linear over the range 0.1-200 microM (r2 > 0.999). Fatty acids in plasma samples were determined after derivatization with DBD-PZ-NH2 and analyzed by LC/MS using standard fatty acid DBD-PZ-NH2 (D) derivatives as internal standards. Furthermore, the relative amounts of fatty acids in two plasma samples were determined after derivatization with DBD-PZ-NH2 and DBD-PZ-NH2) (D). The isotope-labeled derivatization reagent was useful for accurate quantification and the determination of relative amounts of the metabolites in biological samples having the target functional group.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yuhki
authors.initials: Y
authors.lastname: Tsukamoto
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Santa
authors.forename: Hiroo
authors.initials: H
authors.lastname: Yoshida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyano
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fukushima
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hirayama
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Imai
authors.forename: Takashi
authors.initials: T
authors.lastname: Funatsu
mesh_terms: D000818:Animals; D002138:Calibration; D002264:Carboxylic Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003903:Deuterium; D005227:Fatty Acids / Q000097:blood*; D007553:Isotope Labeling / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D010069:Oxadiazoles / Q000138:chemical synthesis*; D010879:Piperazines / Q000138:chemical synthesis*; D051381:Rats
chemical_list: C466110:7-(N,N-dimethylaminosulfonyl)-5-N-(4-N-aminoethyl)piperazino-2,1,3-benzoxadiazole; D002264:Carboxylic Acids; D005227:Fatty Acids; D010069:Oxadiazoles; D010879:Piperazines; D003903:Deuterium