title: Administration of 6-methoxybenzoxazolinone (MBOA) does not augment ovulatory responses in St. Croix White ewes superovulated with PMSG.
abstract: The objective of this investigation was to examine the effects of 6-methoxy-benzoxazolinone (MBOA), a plant compound that resembles melatonin and alters ovarian function in rodents, in combination with PMSG on superovulatory responses in the cycling ewe. In Experiment I, St. Croix White ewes (n = 44) were synchronized (intra-vaginal progestin sponge) for 14days followed by hCG (750 IU) at 1 day after sponge removal (day 0). Ewes were assigned to one of six treatments administered on day -1: Control (no PMSG or MBOA; n = 7); PMSG (1000 IU i.m.; n = 7); Low MBOA (0.43 mg/kg i.m.; n = 7); High MBOA (1.15 mg/kg i.m.; n = 7); Low MBOA + PMSG (n = 8); High MBOA + PMSG (n = 8). In Experiment II, St. Croix White ewes (n = 24) were synchronized (progestin CIDR) for 14 days followed by hCG on day 1 after CIDR removal (day 0). Ewes were assigned to one of three treatments administered on day -1: Control (n = 8); PMSG (n = 8); Low MBOA+PMSG (n = 8). Laparoscopy was performed on day 9 to assess numbers of corpora lutea (CL) and visible follicles on each ovary. Blood samples were collected on day -13, -1, 0, 1, and days 6 or 7-12 for analysis of serum progesterone (P4) by RIA. Treatment groups receiving PMSG (alone or with MBOA) exhibited greater (P < 0.05) serum concentrations of P4 post-synchrony than Control and MBOA-only groups. Ovulation rate was lower (P < 0.05) for Control and MBOA-only treated ewes than ewes receiving PMSG. Ovulation rate in ewes treated with MBOA alone was similar (P > 0.10) to Controls, and PMSG treatment alone did not differ (P > 0.10) from MBOA + PMSG treatment. Ewes treated with PMSG alone did not differ (P > 0.10) in follicle number from High MBOA + PMSG treated ewes, however, Low MBOA + PMSG treated ewes had greater numbers of follicles at day 9 (P < 0.05) than the PMSG or High MBOA + PMSG groups in Experiment I; although, this was not replicated in Experiment II with numbers of follicles in the Low MBOA + PMSG group being similar (P > 0.10) to PMSG alone. In summary, the addition of MBOA in combination with PMSG as part of a synchronization-superovuation protocol in the ewe did not increase ovulation rate.
authors.affiliation: Department of Animal and Dairy Sciences, Mississippi State University, Box 9815-CVM Wise Center, Mississippi State, MS 39762, USA. swillard@ads.msstate.edu
authors.forename: S T
authors.initials: ST
authors.lastname: Willard
authors.forename: T
authors.initials: T
authors.lastname: Dickerson
authors.forename: R
authors.initials: R
authors.lastname: Dodson
authors.forename: A
authors.initials: A
authors.lastname: Weis
authors.forename: R W
authors.initials: RW
authors.lastname: Godfrey
mesh_terms: D000818:Animals; D001583:Benzoxazoles / Q000008:administration & dosage*; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D003338:Corpus Luteum / Q000033:anatomy & histology; D004347:Drug Interactions; D004971:Estrus; D004973:Estrus Synchronization; D005260:Female; D003425:Flurogestone Acetate / Q000008:administration & dosage; D006064:Gonadotropins, Equine / Q000008:administration & dosage*; D006080:Ovarian Follicle / Q000033:anatomy & histology; D011374:Progesterone / Q000097:blood; D012756:Sheep / Q000502:physiology*; D013480:Superovulation / Q000187:drug effects*; D013997:Time Factors
chemical_list: D001583:Benzoxazoles; D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D011374:Progesterone; C031896:6-methoxybenzoxazolinone; D003425:Flurogestone Acetate