Development of an antigen ELISA to detect sapovirus in clinical stool specimens.
Human sapovirus (SaV) strains are etiological agents of mild and/or acute gastroenteritis in children and adults. In this study, we describe the development of a novel antigen enzyme-linked immunosorbent assay (ELISA) detection system that was based on hyperimmune rabbit and guinea pig antisera raised against SaV genogroup I (GI) virus-like particles. The ELISA had 100% specificity, and sensitivities of 60% and 25% when compared to single-round PCR and nested PCR, respectively. Our results have shown the ELISA was useful in detecting SaV GI antigens in clinical stool specimens collected two days after the onset of illness.
Hansman
G S
GS
Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan. ghansman@nih.go.jp
Guntapong
R
R
Pongsuwanna
Y
Y
Natori
K
K
Katayama
K
K
Takeda
N
N
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000821:virology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000175:diagnosis / Q000821:virology; D006168:Guinea Pigs; D006801:Humans; D011817:Rabbits; D029341:Sapovirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral