Hedgehog signaling controls Soma-Germen interactions during Drosophila ovarian morphogenesis.
The genetic analysis of Drosophila adult oogenesis has provided insights into the molecular mechanisms that control cell proliferation, differentiation, migration, and intercellular signaling. However, little is known about the larval and pupal cellular events leading to the formation of the highly organized adult ovary, which is composed of ovarioles each containing germline cells enveloped by specialized somatic cells. We describe here the presence of ovarioles devoid of any germ cells in adult females mutant for fused, which encodes a Hedgehog signal transducing serine/threonine kinase. We show that this phenotype corresponds to a requirement for fused function for the organization of germ cells with respect to ovarian somatic cells during ovariole formation specifically during pupal stages and provide some evidence by means of clonal analysis suggesting that fused function may be necessary in the germline. hedgehog is expressed specifically in somatic terminal filament cells in pupal ovaries, and females bearing hedgehog strong loss-of-function mutations also exhibit aberrant germ cell distribution and formation of agametic ovarioles. These results indicate a positive role for Fused in the transduction of somatic Hedgehog signaling instructing ovariole morphogenesis. We also provide evidence for the use of noncanonical Hedgehog signal transducer(s) within germline cells.
Besse
Florence
F
Institut J. Monod, UMR 7592-CNRS/Université Pierre et Marie Curie/Université Denis Diderot, Laboratoire de Génétique du Développement et Evolution 2, place Jussieu, Paris, France.
Busson
Denise
D
Pret
Anne-Marie
AM
D000483:Alleles; D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D003433:Crosses, Genetic; D004330:Drosophila; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000378:metabolism; D053823:Hedgehog Proteins; D007150:Immunohistochemistry; D008297:Male; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D009024:Morphogenesis; D009154:Mutation; D010053:Ovary / Q000196:embryology* / Q000473:pathology; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000502:physiology*; D011995:Recombination, Genetic; D015398:Signal Transduction; D013997:Time Factors; D019076:Transgenes
D029721:Drosophila Proteins; D053823:Hedgehog Proteins; C077245:hh protein, Drosophila; C085770:fu protein, Drosophila; D017346:Protein Serine-Threonine Kinases