Determination of adenosine disodium triphosphate (ATP) using oxytetracycline-Eu3+ as a fluorescence probe by spectrofluorimetry.
A new spectrofluorimetric method was developed for determination of adenosine disodium triphosphate (ATP). We studied the interactions between oxytetracycline (OTC)-Eu3+ complex and adenosine disodium triphosphate (ATP) by using UV-vis absorption and fluorescence spectra. Using oxytetracycline (OTC)-Eu3+ as a fluorescence probe, under the optimum conditions, ATP can remarkably enhance the fluorescence intensity of the OTC-Eu3+ complex at lambda = 612 nm and the enhanced fluorescence intensity of Eu3+ ion is in proportion to the concentration of ATP. Optimum conditions for the determination of ATP were also investigated. The linear ranges for ATP are 8.00 x 10(-8)-1.50 x 10(-6) mol L(-1) with detection limits of 2.67 x 10(-9) mol L(-1). This method is simple, practical and relatively free interference from coexisting substances and can be successfully applied to determination of ATP in samples. The mechanism of fluorescence enhancement between oxytetracycline (OTC)-Eu3+ complex and ATP was also studied.
Hou
Faju
F
Department of Chemistry, Shandong Normal University, Jinan 250014, China.
Miao
Yanhong
Y
Jiang
Chongqiu
C
D000255:Adenosine Triphosphate / Q000737:chemistry*; D002412:Cations / Q000737:chemistry; D005063:Europium / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010118:Oxytetracycline / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
D002412:Cations; D005456:Fluorescent Dyes; D005063:Europium; D000255:Adenosine Triphosphate; D010118:Oxytetracycline