title: Electrophoretic identification and quantitation of compounds in the polyphenolic fraction of extra-virgin olive oil.
abstract: A capillary zone electrophoresis method has been carried out to determine and quantitate some compounds of the polyphenolic fraction of virgin olive oil which have never previously been determined before using capillary electrophoresis, such as elenolic acid, ligstroside aglycon, oleuropein aglycon, and (+)-pinoresinol. The compounds were identified using standards obtained by semipreparative high-performance liquid chromatography (HPLC). A detailed method optimization was performed to separate the phenolic compounds present in olive oil using a methanol-water extract of Picual extra-virgin olive oil, and different extraction systems were compared (C18-solid phase extraction (SPE), Diol-SPE, Sax-SPE and liquid-liquid extraction). The optimized parameters were 30 mM sodium tetraborate buffer (pH 9.3) at 25 kV with 8 s hydrodynamic injection, and the quantitation was carried out by the use of two reference compounds at two different wavelengths.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain.
authors.forename: Ana María
authors.initials: AM
authors.lastname: Gómez Caravaca
authors.forename: Alegría
authors.initials: A
authors.lastname: Carrasco Pancorbo
authors.forename: Beatriz
authors.initials: B
authors.lastname: Cañabate Díaz
authors.forename: Antonio
authors.initials: A
authors.lastname: Segura Carretero
authors.forename: Alberto
authors.initials: A
authors.lastname: Fernández Gutiérrez
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D005663:Furans / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D057888:Iridoid Glucosides; D039823:Iridoids; D017705:Lignans / Q000032:analysis; D000069463:Olive Oil; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000032:analysis; D010938:Plant Oils / Q000737:chemistry*; D059808:Polyphenols; D011714:Pyrans / Q000032:analysis
chemical_list: D005419:Flavonoids; D005663:Furans; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D017705:Lignans; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D059808:Polyphenols; D011714:Pyrans; C011867:4-hydroxyphenylethanol; C002769:oleuropein; C000352:elenolic acid; C445380:ligstroside; D010626:Phenylethyl Alcohol; C103298:pinoresinol