Isolation and characterization of a novel Bacillus sp., strain YAS1, capable of transforming tyrosol under hypersaline conditions.
A moderately halotolerant, Gram-positive, aerobic, motile, spore-forming bacterium, designated as strain YAS1, was isolated from an olive-brine fermentation rich in aromatic compounds, after enrichment on tyrosol. Strain YAS1 grew between 25 and 45 degrees C and optimally at 37 degrees C. It grew in the presence of 0-15% (v/w) NaCl, with an optimum of 3-6% (v/w) NaCl. The DNA G+C content was found to be 49.9 mol%. Phylogenetic analysis of the 16S rRNA gene revealed that this isolate was a member of the genus Bacillus. The newly isolated strain YAS1 represents the first moderately halotolerant bacterium transforming tyrosol to p-hydroxyphenylacetic acid (PHPA) in the presence of yeast extract.
Abdelkafi
Slim
S
Laboratoire des Bio-procédés, Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 4, 3038 BP "K", Sfax, Tunisie.
Chamkha
Mohamed
M
Casalot
Laurence
L
Sayadi
Sami
S
Labat
Marc
M
D000332:Aerobiosis; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005285:Fermentation; D008969:Molecular Sequence Data; D031658:Olea / Q000382:microbiology*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C011867:4-hydroxyphenylethanol; D012965:Sodium Chloride; D010626:Phenylethyl Alcohol