Global profiling of Streptococcus pneumoniae gene expression at different growth temperatures.
Streptococcus pneumoniae is a common commensal of the upper respiratory tract of healthy humans and is an important pathogen in young children, immunocompromised adults, and the elderly. To better understand the strategies employed by this bacterial species in adapting to conditions present at different infection sites in the host, global transcription profiling was used to study gene expression at different growth temperatures: 21, 29, 33, 37, and 40 degrees C. Here, we found that 658 genes (29%) out of 1717 genes were differently expressed (>or=1.5-fold change) in at least one growth temperature relative to 37 degrees C. The percentages of genes whose expression was altered in each growth temperature, respectively, were: 21 degrees C: 53% upward arrow, 47% downward arrow; 29 degrees C: 44% upward arrow, 56% downward arrow; 33 degrees C: 27% upward arrow, 73% downward arrow and 40 degrees C: 44% upward arrow, 56% downward arrow. Hierarchical clustering (HC) of the temperature regulated genes resulted in four clusters, namely A-D of differently expressed genes grouped by bacterial growth temperature. Cluster A represented 81 genes reflecting enhanced expression at 33 degrees C. Cluster B included 260 genes whose expression increased with growth temperature. Cluster C had 28 genes with 68% showing enhanced expression at 29 degrees C while cluster D had 289 genes with 74% genes showing enhanced expression at 21 degrees C relative to 37 degrees C. Principal component (PC) analysis also divided differentially expressed genes into four groups and was highly correlated with HC, suggesting that temperature regulated expression is not random but coordinated. Overall, these results indicated substantial reprogramming of transcription in response to growth temperature. Functional characterization of differential gene expression at different temperatures provides further information on the molecular mechanism(s) that allows S. pneumoniae to adapt to various host environments.
Pandya
Utpal
U
Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Texas 77555-1075, United States.
Allen
Christopher A
CA
Watson
David A
DA
Niesel
David W
DW
D001426:Bacterial Proteins / Q000235:genetics*; D015415:Biomarkers / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D013290:Streptococcal Infections / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000235:genetics* / Q000472:pathogenicity; D013696:Temperature; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D015415:Biomarkers