In vivo antihepatotoxic effects of Ligularia fischeri var. spiciformis and the identification of the active component, 3,4-dicaffeoylquinic acid.
Pretreatment with a methanolic extract of Ligularia fischeri var. spiciformis (Compositae) herb inhibited hepatotoxicities caused by CCl4, D-galactosamine (GalN), alpha-naphthylisothiocyanate (ANIT), and DL-ethionine in rats. An ethyl acetate (EtOAc) extract fractionated from the methanolic extract showed a strong inhibitory effect. A major component, 3,4-dicaffeoylquinic acid (DCQA), isolated from the methanolic extract was examined for antihepatotoxicity. Pretreatment with DCQA (5 and 10 mg/kg, p.o.) significantly reduced serum aminotransferases (alanine and aspartate), sorbitol dehydrogenase, gamma-glutamyltransferase, alkaline phosphatase, and lactate dehydrogenase activities during CCl4- or GalN-induced hepatotoxicity, suggesting that DCQA is a major principle for the antihepatotoxic activity of L. fischeri var. spiciformis. DCQA also partially restored bile flow and reduced total bilirubin and cholic acid concentrations in rats with ANIT-induced cholestasis. Treatment with DCQA inhibited the increase in triglyceride, cholesterol, and total lipids in DL-ethionine-induced fatty liver. These results support the traditionally held belief that this plant can be used for the treatment of jaundice and hepatic failure.
Choi
Jongwon
J
College of Pharmacy, Kyungsung University, Busan, Korea.
Park
Jung-Kwan
JK
Lee
Kyung-Tae
KT
Park
Kwang-Kyun
KK
Kim
Won-Bae
WB
Lee
Jin-Ha
JH
Jung
Hyun-Ju
HJ
Park
Hee-Juhn
HJ
D015058:1-Naphthylisothiocyanate / Q000633:toxicity; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D019659:Asteraceae / Q000737:chemistry*; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury; D002726:Chlorogenic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis; D005001:Ethionine / Q000633:toxicity; D005234:Fatty Liver / Q000097:blood / Q000139:chemically induced; D005688:Galactosamine / Q000633:toxicity; D007064:L-Iditol 2-Dehydrogenase / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008055:Lipids / Q000097:blood; D008099:Liver / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology / Q000517:prevention & control*; D008297:Male; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D005723:gamma-Glutamyltransferase / Q000097:blood
D008055:Lipids; D002726:Chlorogenic Acid; D015058:1-Naphthylisothiocyanate; D005688:Galactosamine; D002251:Carbon Tetrachloride; C478100:3,4-di-O-caffeoylquinic acid; D007064:L-Iditol 2-Dehydrogenase; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D005001:Ethionine