title: Feasibility of using fluoresceinyl Cypridina luciferin analog in a novel chemiluminescence method for real-time photodynamic therapy dosimetry.
abstract: Singlet oxygen ((1)O(2)) is the most important cytotoxic agent in photodynamic therapy (PDT). The feasibility of using a chemiluminescence (CL) probe, 3,7-dihydro-6-[4-(2-(N'-(5-fluoresceinyl)thioureido)ethoxy)phenyl]-2-methylimidazo{1,2-a}pyrazin-3-one sodium salt (fluoresceinyl Cypridina luciferin analog, FCLA), to monitor (1)O(2) production during PDT is evaluated in vitro. Lymphoma cells were treated with various protocols of PDT. The results show that the FCLA-CL production during PDT is linearly related to the corresponding cytotoxicity, regardless of the treatment protocol. With minimum cytotoxicity and interference to the PDT treatment outcome, the FCLA-CL system is an effective means to quantify PDT (1)O(2) production and may provide an alternative real-time dosimeter.
authors.affiliation: Institute of Laser Life Science, South China Normal University, Guangzhou, 510631, PR China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Qin
authors.forename: Da
authors.initials: D
authors.lastname: Xing
authors.forename: Shiming
authors.initials: S
authors.lastname: Luo
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Xueyun
authors.initials: X
authors.lastname: Zhong
authors.forename: Qun
authors.initials: Q
authors.lastname: Chen
mesh_terms: D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D017323:Dihematoporphyrin Ether / Q000737:chemistry; D005240:Feasibility Studies; D005452:Fluoresceins / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D049449:Luminescence; D010778:Photochemotherapy / Q000379:methods; D011874:Radiometry; D026082:Singlet Oxygen / Q000737:chemistry; D019810:Sodium Azide / Q000737:chemistry / Q000494:pharmacology
chemical_list: D005452:Fluoresceins; D007093:Imidazoles; C080213:3,7-dihydro-6-(4-(2-(N'-(5-fluoresceinyl)thioureido)ethoxy)phenyl)-2-methylimidazo-(1,2-a)pyrazin-3-one; D026082:Singlet Oxygen; D019810:Sodium Azide; D017323:Dihematoporphyrin Ether