title: Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens.
abstract: We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.
authors.affiliation: Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: A T
authors.initials: AT
authors.lastname: Hesselink
authors.forename: A J C
authors.initials: AJ
authors.lastname: van den Brule
authors.forename: Z M A
authors.initials: ZM
authors.lastname: Groothuismink
authors.forename: M
authors.initials: M
authors.lastname: Molano
authors.forename: J
authors.initials: J
authors.lastname: Berkhof
authors.forename: C J L M
authors.initials: CJ
authors.lastname: Meijer
authors.forename: P J F
authors.initials: PJ
authors.lastname: Snijders
mesh_terms: D002584:Cervix Uteri / Q000821:virology*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase; D014626:Vaginal Smears
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral; D019914:Taq Polymerase