[Establishment and characterization of a melanoma cell line HME1].
OBJECTIVE
To establish a melanoma cell line and identify its characteristics in vitro.


METHODS
The tissues from biopsies of melanoma were performed primary culture. After growing to 90% confluence, the cells were detached and transferred to another flask for subculture, and then we identified their characteristics including growth kinetic, morphology and tumorigenecity.


RESULTS
This cell line was cultivated for more than 90 times. Its characteristics were as follows:The pathological morphology of the tumor transplanted in nude mice were similar to that of the original lesion;The growth curves of the 20th passage were determined, and the population doubling time calculated was 56.9 h; The cloning efficiency in soft agar was 19.1%; Karyo-type analysis showed aneuploidy with the modal chromosomal number 85-102; Electronmicroscopical observation showed that there were rich microvilli on the surface of the cells, abundant ribosomes and melanoid grain; SP immunohistochemical staining showed that the cells expressed HMB-45.


CONCLUSIONS
The Melanoma cells were immortalized after being cultured in vitro and a new melanoma cell line was established.
Wu
Shang-Hui
SH
Department of Cell Biology, Xiangya School of Medicine, Central South University, Changsha 410078, China. wsh8856@163.com
Huang
Bai-Ying
BY
Gu
Huan-Hua
HH
D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D008545:Melanoma / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D012878:Skin Neoplasms / Q000473:pathology*