title: Germline polymorphism of p53 codon 72 in gynecological cancer.
abstract: OBJECTIVE
  To investigate the biological significance of single nucleotide polymorphism at codon 72 of the p53 gene in the development of gynecological cancer.
  
  
  METHODS
  p53 codon 72 polymorphism was examined in a total of 354 blood samples from 95 normal, 83 cervical, 108 endometrial and 68 ovarian cancer cases using polymerase chain reaction and restriction fragment length polymorphism techniques.
  
  
  RESULTS
  When p53 codon 72 genotype was classified into two subgroups of Arg/Arg and Arg/Pro + Pro/Pro, the Arg/Arg genotype was associated with an increased risk for the development of endometrial cancer (OR = 1.86, 95% CI = 1.06 to 3.26) compared with the Arg/Pro + Pro/Pro genotype (P = 0.0301). The Arg allele also increased the risk of endometrial cancer (OR = 1.42, 95% CI = 0.93 to 1.52) compared with the Pro allele, but no statistical difference was found (P = 0.1031). There was no significant difference in the genotype or allele prevalence between control subjects and cervical or ovarian cancer patients.
  
  
  CONCLUSIONS
  Homozygous Arg at codon 72 of the p53 gene may be a risk factor for developing endometrial cancer in a Japanese population.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka Medical College, 2-7 Daigakumachi, Takatsuki Osaka 569-8686, Japan. gyn017@poh.osaka-med.ac.jp
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Ueda
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Terai
authors.forename: Koji
authors.initials: K
authors.lastname: Kanda
authors.forename: Masanori
authors.initials: M
authors.lastname: Kanemura
authors.forename: Mikio
authors.initials: M
authors.lastname: Takehara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Koji
authors.initials: K
authors.lastname: Nishiyama
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yasuda
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueki
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D003062:Codon; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D005833:Genital Neoplasms, Female / Q000235:genetics*; D005838:Genotype; D018095:Germ-Line Mutation; D006801:Humans; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D003062:Codon