[Study on the active protein fractions from scorpii tegument].
OBJECTIVE
Extracting the total protein from scorpii tegument, investigating its effect on immune system by transformation of T/B cell.


METHODS
Water-soluble protein(ST1) was extracted by distilled water method and salting-out method, while keratin(ST2) by deoxidization method. Sephadex G-50 was used to isolate the protein. The effects of components isolated by sephadex G-50 on T/B and NK cell were investigated.


RESULTS
The protein from scorpii tegument could increase the transformation of T/B cell distinctly in vitro, while no apparent effect on NK cell.


CONCLUSIONS
Protein from scorpii tegument could modulate the immune system, which may offer a new way for people to find protein drugs.
Wu
Li
L
Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China.
Zhang
Li
L
Du
Guan-Hua
GH
Lu
Yang
Y
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D005260:Female; D007633:Keratins / Q000302:isolation & purification* / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D012605:Scorpions / Q000737:chemistry*; D012867:Skin / Q000737:chemistry; D013601:T-Lymphocytes / Q000187:drug effects
D011506:Proteins; D007633:Keratins