title: Coordinates, DNA content and heterogeneity of cell nuclei and segments of the Caenorhabditis elegans intestine.
abstract: A specific protocol allowed to selectively stain and image the cell nuclei of the intestine of the nematode Caenorhabditis elegans. Digital processing of these images yielded quantitative information on the coordinates and the relative DNA content of these cell nuclei. With this technique, the exact locations (in relation to the length of worm or intestine) of the 20 intestinal cell nuclei of larval stage L1 and of the 34 intestinal cell nuclei of larval stages L2-4 and adults were determined. Tracking the DNA content of an individual intestinal cell nuclei allowed to study nuclear DNA endoreduplications during larval growth. The evaluation of the DNA content of all intestinal cell nuclei revealed a strong heterogeneity between the cell nuclei and intestinal segments with a maximum in segments int4-int5 in the L1 stage and int5-int6 in the L2-L4 stages. Minimum values were detected in the segments int2 and int8-int9 of all larval stages. Heterogeneity between the intestinal segments of C. elegans does not only concern the DNA content of cell nuclei, but also other cellular features like the quantity of intracellular vesicles or the absorption of particles out of the intestinal lumen.
authors.affiliation: Institut für Zoophysiologie, Westfälische Wilhelms-Universität Münster, Hindenburgplatz 55, 48143, Münster, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Wolf
authors.forename: Frank
authors.initials: F
authors.lastname: Nunes
authors.forename: Rüdiger J
authors.initials: RJ
authors.lastname: Paul
mesh_terms: D000818:Animals; D006690:Bisbenzimidazole / Q000737:chemistry; D017173:Caenorhabditis elegans / Q000502:physiology*; D002455:Cell Division; D002467:Cell Nucleus / Q000737:chemistry*; D018509:DNA, Helminth / Q000032:analysis* / Q000096:biosynthesis; D005456:Fluorescent Dyes / Q000737:chemistry; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007422:Intestines / Q000737:chemistry* / Q000166:cytology; D007814:Larva; D018715:Microscopy, Video
chemical_list: D018509:DNA, Helminth; D005456:Fluorescent Dyes; D006690:Bisbenzimidazole