Y. enterocolitica translocated Yops impair stimulation of T-cells by antigen presenting cells.
As T helper cells play a crucial role in the defense of the mouse immune system against Yersinia enterocolitica, an effective subversion strategy for the pathogen would be the inhibition of T-cell activation. In this study, we investigated whether Y. enterocolitica impairs this process on the level of antigen presentation. For this purpose, we used T-cells to measure the antigen presentation capacity of dendritic cells after they had been incubated with different types of Yersinia mutants. We could show that Y. enterocolitica impairs the processing of antigens by dendritic cells, that this effect is dependent on factors translocated by the pathogenicity-plasmid-encoded type III secretion system and that the most important factor appears to be YopP. The YopP effect is partly mediated by the killing of APCs, but in addition to this there appears to be an alternative way of action that results in the inhibition of antigen processing. The YopP effect is not mediated by soluble factors. In contrast to antigen processing, antigen presentation was only weakly affected by pathogenicity plasmid encoded factors in dendritic cells, but obviously in A20.J B-cells.
Kramer
Uwe
U
Department of Medical Microbiology and Hygiene, University of Tübingen, Germany. uwe.kramer@fgk-cro.de
Wiedig
Carolin A
CA
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D017209:Apoptosis; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000382:microbiology*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009154:Mutation; D010047:Ovalbumin / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D010957:Plasmids; D013601:T-Lymphocytes / Q000276:immunology*; D015008:Yersinia enterocolitica / Q000235:genetics / Q000472:pathogenicity*
D001426:Bacterial Proteins; C111390:OVA 323-339; D010446:Peptide Fragments; C109227:YopP protein, Yersinia; D010047:Ovalbumin