Prenatal hypoxia impairs memory function but does not result in overt structural alterations in the postnatal chick brain.
We showed previously that hypoxia in ovo impairs memory consolidation in the chick tested 2 days after hatching. Our present aim was to investigate whether we could detect any morphological effects of the same prenatal hypoxia. Hypoxia was induced by half-wrapping the egg with an impermeable membrane from either days 10-18 (W10-18 chicks) or days 14-18 (W14-18 chicks) of incubation (hatching approximately 21 days). Measurement of blood gases showed that reducing the surface area of the egg for gas exchange resulted in reduced pO2 and increased pCO2 2 days after wrapping. Although this hypoxia was sufficient to impair cognitive processing in the postnatal chick, our data suggest that it did not produce overt structural alterations or changes in the number of neurons, glutamine synthetase-immunoreactive cells or immunoreactivity to synaptophysin in the presynaptic vesicles in the multimodal integration (cortical) area compared to controls. Hence, we found no differences in the astrocyte to neuron ratio, synaptic density and/or vesicle number. Analysis of the ontogeny of astrocytes during the prenatal period of hypoxia showed them to be present at embryonic day 12, but not at the earlier ages examined. Although we found cognitive deficits in chicks from embryos made hypoxic during incubation, our regimen of prenatal hypoxia did not alter any of the parameters measured in the brains. This does not preclude the possibility that changes have occurred at the cellular or molecular levels or in specific neurotransmitter systems.
Camm
Emily J
EJ
Fetal and Neonatal Research Group, Department of Physiology, Monash University, Victoria 3800, Australia.
Gibbs
Marie E
ME
Harding
Richard
R
Mulder
Twan
T
Rees
Sandra M
SM
D000818:Animals; D020520:Brain Infarction / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D002642:Chick Embryo; D002645:Chickens; D004195:Disease Models, Animal; D005311:Fetal Hypoxia / Q000473:pathology / Q000503:physiopathology*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D002534:Hypoxia, Brain / Q000473:pathology / Q000503:physiopathology*; D007150:Immunohistochemistry; D008569:Memory Disorders / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D046529:Microscopy, Electron, Transmission; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000378:metabolism / Q000648:ultrastructure; D016708:Synaptophysin / Q000378:metabolism
D016708:Synaptophysin; D018698:Glutamic Acid; D005974:Glutamate-Ammonia Ligase