Strain differences in myenteric neuron number and CCK1 receptor mRNA expression may account for differences in CCK induced c-Fos activation.
We utilized a diaminobenzidine reaction enhanced with nickel to compare dorsal vagal complex (DVC) and myenteric neuronal Fos-Like immunoreactivity (Fos-LI), in response to sulfated cholecystokinin-8 (CCK-8) (5, 10, 20, 40 microg/kg), among Sprague-Dawley (SD), Standard Long-Evans (SLE), Otsuka Long-Evans Tokushima Fatty (OLETF), and Long-Evans Tokushima Otsuka (LETO) rats. All rat strains but OLETF expressed Fos-LI in response to CCK-8. In addition, SD rats expressed more Fos-LI in the area postrema and myenteric neurons than SLE and LETO rats. To investigate the basis for these differences, we utilized cuprolinic blue staining, which stains neuronal cell bodies, to quantify the number of myenteric neurons, and a reverse transcriptase chain polymerase reaction to measure the gene expression of CCK(1) receptor in the gut. We found that SD rats have significantly more duodenal myenteric neurons than the other strains. In addition, this strain expressed significantly higher levels of the CCK(1) gene in both the duodenum and jejunum than the other strains. In conclusion, SD rats may express more myenteric Fos-LI in response to CCK due to increased numbers of myenteric neurons or more intestinal CCK(1) receptors than the other strains of rats.
Gulley
Stephen
S
Department of Biomedical Sciences, Gastroenterology Laboratory, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL 36088, USA.
Sharma
Sanjay K
SK
Mansour
Mahmoud
M
Sullivan
Cherese N
CN
Moran
Timothy H
TH
Sayegh
Ayman I
AI
D000818:Animals; D002452:Cell Count; D002766:Cholecystokinin / Q000378:metabolism* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D007150:Immunohistochemistry; D009197:Myenteric Plexus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D020334:Rats, Inbred OLETF; D020318:Rats, Long-Evans; D017207:Rats, Sprague-Dawley; D011949:Receptors, Cholecystokinin / Q000235:genetics* / Q000378:metabolism*; D012844:Sincalide / Q000378:metabolism / Q000494:pharmacology; D013045:Species Specificity
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D011949:Receptors, Cholecystokinin; D002766:Cholecystokinin; D012844:Sincalide