[Cytotoxicity study of heparin coated polyvinyl chloride material].
In this study, heparin ionically coated polyvinyl chloride (PVC) material was prepared by heparin-benzalkonium chloride complex (Group A), heparin-benzalkonium bromide complex (Group B) and heparin-polyethyleneimine compound (Group C). Cytotoxicity evaluation was conducted by direct cell contact evaluation and MTT colorimetry method. The results showed Group A and Group B caused L-929 cells to die out while Group C showed good compatibility with cells. The OD levels of Group A and B were lower than that of the Group C in MTT test. Method A and method B of heparin coating had remarkable cytotoxicity, while method C had little cytotoxicity and could be further studied for clinical use.
Yang
Jian
J
Center of Cardiovascular Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710033, China. yangjian1212@hotmail.com
Yi
Dinghua
D
Liu
Jincheng
J
Liu
Jian
J
Cao
Ruijun
R
Qin
Feng
F
D000818:Animals; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000633:toxicity*; D005347:Fibroblasts / Q000166:cytology*; D006493:Heparin / Q000633:toxicity*; D008422:Materials Testing; D051379:Mice; D011143:Polyvinyl Chloride / Q000633:toxicity*
D020099:Coated Materials, Biocompatible; D011143:Polyvinyl Chloride; D006493:Heparin