title: Streptococcus iniae phosphoglucomutase is a virulence factor and a target for vaccine development.
abstract: Streptococcus iniae represents a major health and economic problem in fish species worldwide. Random Tn917 mutagenesis and high-throughput screening in a hybrid striped bass (HSB) model of meningoencephalitis identified attenuated S. iniae mutants. The Tn917 insertion in one mutant disrupted an S. iniae homologue of a phosphoglucomutase (pgm) gene. Electron microscopy revealed a decrease in capsule thickness and cell wall rigidity, with DeltaPGM mutant cells reaching sizes approximately 3-fold larger than those of the wild type (WT). The DeltaPGM mutant was cleared more rapidly in HSB blood and was more sensitive to killing by cationic antimicrobial peptides including moronecidin from HSB. In vivo, the DeltaPGM mutant was severely attenuated in HSB, as intraperitoneal challenge with 1,000 times the WT lethal dose produced only 2.5% mortality. Reintroduction of an intact copy of the S. iniae pgm gene on a plasmid vector restored antimicrobial peptide resistance and virulence to the DeltaPGM mutant. In analysis of the aborted infectious process, we found that DeltaPGM mutant organisms initially disseminated to the blood, brain, and spleen but were eliminated by 24 h without end organ damage. Ninety to 100% of fish injected with the DeltaPGM mutant and later challenged with a lethal dose of WT S. iniae survived. We conclude that the pgm gene is required for virulence in S. iniae, playing a role in normal cell wall morphology, surface capsule expression, and resistance to innate immune clearance mechanisms. An S. iniae DeltaPGM mutant is able to stimulate a protective immune response and may have value as a live attenuated vaccine for aquaculture.
authors.affiliation: Department of Pediatrics, University of California, San Diego, School of Medicine, La Jolla, 92093, USA. jtbuchan@ucsd.edu
authors.forename: John T
authors.initials: JT
authors.lastname: Buchanan
authors.forename: Jason A
authors.initials: JA
authors.lastname: Stannard
authors.forename: Xavier
authors.initials: X
authors.lastname: Lauth
authors.forename: Vaughn E
authors.initials: VE
authors.lastname: Ostland
authors.forename: Henry C
authors.initials: HC
authors.lastname: Powell
authors.forename: Mark E
authors.initials: ME
authors.lastname: Westerman
authors.forename: Victor
authors.initials: V
authors.lastname: Nizet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology; D001492:Bass / Q000382:microbiology; D005393:Fish Diseases / Q000382:microbiology / Q000517:prevention & control*; D029941:Fish Proteins / Q000494:pharmacology; D017353:Gene Deletion; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D010733:Phosphoglucomutase / Q000235:genetics*; D013290:Streptococcal Infections / Q000517:prevention & control / Q000662:veterinary*; D022541:Streptococcal Vaccines / Q000276:immunology; D013291:Streptococcus / Q000187:drug effects / Q000235:genetics* / Q000276:immunology*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D023181:Antimicrobial Cationic Peptides; D029941:Fish Proteins; D022541:Streptococcal Vaccines; D037521:Virulence Factors; C449802:moronecidin protein, Morone saxatilis; D010733:Phosphoglucomutase