Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library.
A 10-mer overlapping peptide library has been synthesized for screening and identification of linear B-cell epitopes of severe acute respiratory syndrome associated coronavirus (SARS-CoV), which spanned the major structural proteins of SARS-CoV. One hundred and eleven candidate peptides were positive according to the result of PEPscan, which were assembled into 22 longer peptides. Five of these peptides showed high cross-immunoreactivities (approximately 66.7 to 90.5%) to SARS convalescent patients' sera from the severest epidemic regions of the China mainland. Most interestingly, S(471-503), a peptide located at the receptor binding domain (RBD) of SARS-CoV, could specifically block the binding between the RBD and angiotensin-converting enzyme 2, resulting in the inhibition of SARS-CoV entrance into host cells in vitro. The study demonstrated that S(471-503) peptide was a potential immunoantigen for the development of peptide-based vaccine or a candidate for further drug evaluation against the SARS-CoV virus-cell fusion.
Hu
Hongbo
H
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, 1 Xian Nong Tan Street, Beijing 100050, P. R. China.
Li
Li
L
Kao
Richard Y
RY
Kou
Binbin
B
Wang
Zhanguo
Z
Zhang
Liang
L
Zhang
Huiyuan
H
Hao
Zhiyong
Z
Tsui
Wayne H
WH
Ni
Anping
A
Cui
Lianxian
L
Fan
Baoxing
B
Guo
Feng
F
Rao
Shuan
S
Jiang
Chengyu
C
Li
Qian
Q
Sun
Manji
M
He
Wei
W
Liu
Gang
G
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D020650:Combinatorial Chemistry Techniques; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D019151:Peptide Library; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry* / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology
D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; D019151:Peptide Library