title: Maintenance of immune tolerance depends on normal tissue homeostasis.
abstract: Ags expressed at immune privileged sites and other peripheral tissues are able to induce T cell tolerance. In this study, we analyzed whether tolerance toward an intraocular tumor expressing a highly immunogenic CTL epitope is maintained, broken, or reverted into immunity in the event the anatomical integrity of the eye is lost. Inoculation of tumor cells into the anterior chamber of the eye of naive B6 mice leads to progressive intraocular tumor growth, an abortive form of CTL activation in the tumor-draining submandibular lymph node, and systemic tolerance as evidenced by the inability of these mice to reject an otherwise benign tumor cell inoculum. Loss of anatomical integrity of the eye as a consequence of phthisis resulted in loss of systemic tolerance and the emergence of effective antitumor immunity against an otherwise lethal tumor challenge. Phthisis was accompanied by dendritic cell maturation and preceded the induction of systemic tumor-specific CTL immunity. Our data show that normal tissue homeostasis and anatomical integrity is required for the maintenance of ocular tolerance and prevention of CTL-mediated immunity. These data also indicate that tissue injury in the absence of viral or microbial infection can act as a switch for the induction of CTL immunity.
authors.affiliation: Department of Ophthalmology.
authors.forename: Zita F H M
authors.initials: ZF
authors.lastname: Boonman
authors.forename: Geertje J D
authors.initials: GJ
authors.lastname: van Mierlo
authors.forename: Marieke F
authors.initials: MF
authors.lastname: Fransen
authors.forename: Rob J W
authors.initials: RJ
authors.lastname: de Keizer
authors.forename: Martine J
authors.initials: MJ
authors.lastname: Jager
authors.forename: Cornelis J M
authors.initials: CJ
authors.lastname: Melief
authors.forename: René E M
authors.initials: RE
authors.lastname: Toes
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000276:immunology; D017951:Antigen Presentation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000276:immunology; D039521:CD11c Antigen / Q000378:metabolism; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D045142:Cross-Priming / Q000276:immunology; D005134:Eye Neoplasms / Q000276:immunology*; D006706:Homeostasis / Q000276:immunology*; D007108:Immune Tolerance / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D039521:CD11c Antigen