Multicentre survey of the in-vitro activity of seven antimicrobial agents, including ertapenem, against recently isolated Gram-negative anaerobic bacteria in Greece.
The in-vitro activities of penicillin, ticarcillin-clavulanic acid, cefoxitin, imipenem, ertapenem, metronidazole and clindamycin were evaluated against 138 Gram-negative anaerobic isolates (82 Bacteroides fragilis group, 17 non-fragilis Bacteroides spp., 31 Prevotella spp., four Fusobacterium spp., two Veillonella spp., one Porphyromonas sp. and one Tissierella praeacuta) collected from six general hospitals in Athens, Greece. Overall rates of non-susceptibility (both resistant and intermediately-resistant) to penicillin and ticarcillin-clavulanic acid were 81.8% and 2.3%, respectively. The rates of non-susceptibility to cefoxitin and clindamycin were 30.3% and 31.1%, respectively, and that for metronidazole was 4.3% (four Prevotella spp. isolates, one Porphyromonas sp. isolate and one B. fragilis isolate). Only the single B. fragilis isolate was nim-positive by PCR. Only one B. fragilis isolate was resistant to both carbapenems tested, while six more Bacteroides spp. isolates were imipenem-susceptible and ertapenem-non-susceptible. The MIC range, MIC(50) and MIC(90) values were comparable for imipenem and ertapenem, although ertapenem MIC(90)s were one or two two-fold dilutions higher.
Papaparaskevas
J
J
Department of Microbiology, Laikon General Hospital, National and Kapodistrian University of Athens, Athens, Greece. ipapapar@med.uoa.gr
Pantazatou
A
A
Katsandri
A
A
Legakis
N J
NJ
Avlamis
A
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001421:Bacteria, Anaerobic / Q000187:drug effects* / Q000302:isolation & purification; D001441:Bacteroides fragilis / Q000187:drug effects; D015780:Carbapenems / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D000077727:Ertapenem; D006089:Gram-Negative Anaerobic Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006115:Greece; D006801:Humans; D020128:Inhibitory Concentration 50; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D047090:beta-Lactams / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D015780:Carbapenems; D047090:beta-Lactams; D008795:Metronidazole; D000077727:Ertapenem