title: Heterodimerization of ecdysone receptor and ultraspiracle on symmetric and asymmetric response elements.
abstract: Heterodimerization of nuclear receptors is facilitated by the interaction of two dimerization interfaces: one spanning the DNA-binding (C domain) region and the adjacent hinge (D domain) region, and the other in the ligand-binding (E domain) region. Ultraspiracle (USP) heterodimerizes with ecdysone receptor (EcR) and this complex participates in ecdysone signal transduction. The natural ecdysone response elements (EcREs) discovered so far are asymmetric elements composed of either imperfect palindromes or direct repeats. However, gel mobility shift assays have shown that both symmetric (perfect palindromes) and asymmetric (imperfect palindromes and direct repeats) elements can bind to the EcR/USP complex. Therefore, we analyzed EcR/USP domains involved in heterodimerization on different types of response elements (RE). Gel shift assays using full-length and truncated EcR and USP proteins showed that heterodimerization of these two proteins in the presence of asymmetric RE (DR4 and the natural EcRE hsp27) requires both dimerization interfaces present in CD and E domains of both proteins. In contrast, the dimerization interface present in the E domain of either EcR or USP was not essential for heterodimerization on symmetric RE such as PAL1 or IR1. We conclude that the use of heterodimerization interfaces present in CD and E domains of EcR/USP depends on the nature of response elements they bind to.
authors.affiliation: Great Lakes Forestry Centre, Canadian Forestry Service, Sault Ste. Marie, Ontario, Canada.
authors.forename: Srini C
authors.initials: SC
authors.lastname: Perera
authors.forename: Sichun
authors.initials: S
authors.lastname: Zheng
authors.forename: Qi-Li
authors.initials: QL
authors.lastname: Feng
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Krell
authors.forename: Arthur
authors.initials: A
authors.lastname: Retnakaran
authors.forename: Subba R
authors.initials: SR
authors.lastname: Palli
mesh_terms: D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D019281:Dimerization; D029721:Drosophila Proteins; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism; D007915:Lepidoptera / Q000235:genetics; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D011987:Receptors, Steroid / Q000737:chemistry* / Q000378:metabolism*; D020218:Response Elements / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D011987:Receptors, Steroid; D014157:Transcription Factors; C066030:USP protein, Drosophila; C055469:ecdysone receptor