title: Simultaneous liquid chromatographic assay of amantadine and its four related compounds in phosphate-buffered saline using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent derivatization reagent.
abstract: Simultaneous HPLC assay of 1-adamantanamine hydrochloride (amantadine) and its four related compounds [2-adamantanamine hydrochloride (2-ADA), 1-adamantanmethylamine (ADAMA), 1-(1-adamantyl)ethylamine hydrochloride (rimantadine) and 3,5-dimethyl-1-adamantanamine hydrochloride (memantine)] in phosphate-buffered saline (pH 7.4) after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was developed. Phosphate-buffered saline samples were mixed with borate buffer and NBD-F solution in acetonitrile at 60 degrees C for 5 min and injected into HPLC. Five derivatives were well separated from each other. The lower limits of detection of amantadine, 2-ADA, ADAMA, rimantadine and memantine were 0.008, 0.001, 0.0008, 0.0015 and 0.01 microg/mL, respectively. The coefficients of variation for intra- and inter-day assay were less than 6.4 and 8.2%, respectively. The method presented was applied to a binding study of these compounds to human alpha(1)-acid glycoprotein. While affinity constants and capacities for ADAMA, rimantadine and memantine were calculated by means of Scatchard plots, those for the others were not determined. ADAMA, rimantadine and memantine were bound with different affinities and capacities. These results indicate that NBD-F is a good candidate as a fluorescent reagent to simultaneously determine amantadine and its four related compounds by HPLC after pre-column derivatization. Our method can be applied to binding studies for protein.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3, Kanagawa-machi, Kanazawa 920-1181, Japan. y-higashi@hokuriku-u.ac.jp
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Higashi
authors.forename: Shota
authors.initials: S
authors.lastname: Nakamura
authors.forename: Hiroki
authors.initials: H
authors.lastname: Matsumura
authors.forename: Youichi
authors.initials: Y
authors.lastname: Fujii
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000737:chemistry; D000547:Amantadine / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*; D001667:Binding, Competitive; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008559:Memantine / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D010710:Phosphates / Q000737:chemistry; D015203:Reproducibility of Results; D012299:Rimantadine / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D010710:Phosphates; D012299:Rimantadine; D012965:Sodium Chloride; D000547:Amantadine; D009327:4-Chloro-7-nitrobenzofurazan; D008559:Memantine