[Expression of intercellular adhesion molecule-1 and neutrophil infiltration induced by injection of thrombin in rats].
OBJECTIVE
To investigate the role of the thrombin in inducing inflammatory response in brain tissue adjacent to hematoma in the brain.


METHODS
Seventy-two male Wistar rats were randomly divided into two groups: sham-operated control group and thrombin injection group. Immunohistochemical technique and myeloperoxidase (MPO) determination were respectively used to evaluate intercellular adhesion molecule-1 (ICAM-1) expression and neutrophil infiltration in adjacent brain tissue of the thrombin injection zone at 6, 12, 24, 48, 72 and 128 hours after thrombin injection.


RESULTS
The expression of ICAM-1 in tissues adjacent to thrombin injection started to increase 6 hours after the injection, peaking at 72 hours, and lasting for 120 hours. MPO activity of tissues in the area increased 6 hours, reached a high level at 24 hours, then decreased slightly, and it peaked at 72 hours after thrombin injection, correlating closely with the increase in ICAM-1 expression except the changes at 24 hours.


CONCLUSIONS
The increased expression of ICAM-1 after thrombin injection is correlated with the degree of the neutrophil infiltration in the brain tissue around the thrombin injection zone, indicating thrombin is important to produce secondary brain damage after cerebral hemorrhage.
Gao
Chang-yue
CY
Department of Neurology, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Zhou
Hua-dong
HD
Chen
Man-e
ME
D000818:Animals; D002543:Cerebral Hemorrhage / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D020556:Neutrophil Infiltration / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013917:Thrombin / Q000494:pharmacology*
D018799:Intercellular Adhesion Molecule-1; D013917:Thrombin