Effects of propyl gallate on interaction between TNF-alpha and sTNFR-I using an affinity biosensor.
OBJECTIVE
To study the effects of propyl gallate on the interaction of tumor necrosis factor-alpha (TNF-alpha) with its soluble receptor, sTNFR-I.


METHODS
Interactions between TNF-alpha and sTNFR-I were analyzed using an IAsys biosensor. sTNFR-I was immobilized on the carboxymethyl dextran (CMD) surface of the IAsys biosensor cuvettes, and TNF-alpha preincubated with different concentrations of propyl gallate was added to the cuvettes. The resonant angle shift caused by the binding between TNF-alpha and sTNFR-I was then recorded.


RESULTS
sTNFR-I was immobilized on the CMD surface at a density of 2.76 ng/mm(2). TNF-alpha then bound the immobilized sTNFR-I specifically, and propyl gallate was able to enhance the binding between TNF-alpha and sTNFR-I in a dose-dependent manner.


CONCLUSIONS
The binding between TNF-alpha and sTNFR-I is one of the targets that propyl gallate can act on in vivo. The IAsys biosensor offers a new clue as to the study on the mechanisms of action of propyl gallate.
Li
Jing
J
Laboratory of Biosensors, College of Life Science, Nankai University, Tianjin 300071, China.
Huang
Jia-dong
JD
Wu
Bo-yan
BY
Chen
Qiang
Q
D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000379:methods; D004305:Dose-Response Relationship, Drug; D011435:Propyl Gallate / Q000008:administration & dosage / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000975:Antioxidants; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha; D011435:Propyl Gallate