title: Rapid and quantitative detection of hepatitis A virus from green onion and strawberry rinses by use of real-time reverse transcription-PCR.
abstract: In this study, an immunomagnetic capture method and a real-time reverse transcription-PCR assay were used to quantify hepatitis A virus (HAV) in green onion and strawberry rinses. This combined protocol detected as low as 0.5 PFU HAV in produce rinses and concentrated HAV levels up to 20-fold.
authors.affiliation: Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada. mgriffit@uoguelph.ca.
authors.forename: X C
authors.initials: XC
authors.lastname: Shan
authors.forename: P
authors.initials: P
authors.lastname: Wolffs
authors.forename: M W
authors.initials: MW
authors.lastname: Griffiths
mesh_terms: D002021:Buffers; D031985:Fragaria / Q000821:virology*; D030041:Hepatitis A virus / Q000235:genetics / Q000302:isolation & purification*; D018189:Immunomagnetic Separation; D019697:Onions / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
chemical_list: D002021:Buffers; D012367:RNA, Viral