Periplasmic protein-protein contacts in the inner membrane protein Wzc form a tetrameric complex required for the assembly of Escherichia coli group 1 capsules.
The K antigenic capsular polysaccharide forms a structural layer, the capsule, on the surfaces of Escherichia coli cells. The capsule provides an important protective covering that helps protect encapsulated bacteria from host immune defenses. The assembly and translocation of the capsule requires proteins in the inner and outer membranes. The inner membrane protein Wzc is a tyrosine autokinase that plays an essential role in what is believed to be a coordinated biosynthesis and secretion process. Mutants lacking Wzc can form K antigen oligosaccharides but are unable to polymerize high molecular weight capsular polymers. Wzc homologs have been identified in exopolymer biosynthesis systems in many different Gram-negative and -positive bacteria. Using single particle averaging on cryo-negatively stained samples, we have produced the first three-dimensional structure of this type of membrane protein in its phosphorylated state at approximately 14 A resolution. Perfluoro-octanoate-PAGE analysis of detergent-solubilized oligomeric Wzc and symmetry analysis of the transmission electron microscopy data clearly demonstrated that Wzc forms a tetrameric complex with C4 rotational symmetry. Viewed from the top of the complex, the oligomer is square with a diameter of approximately 100 A and can be divided into four separate densities. From the side, Wzc is approximately 110 A high and has a distinctive appearance similar to an extracted molar tooth. The upper "crown" region is approximately 55 A high and forms a continuous ring of density. Four unconnected "roots" ( approximately 65 A high) emerge from the underside of the crown. We propose that the crown is formed by protein-protein contacts from the four Wzc periplasmic domains, while each root represents an individual cytoplasmic tyrosine autokinase domain.
Collins
Richard F
RF
Faculty of Life Science, University of Manchester, Manchester M60 1QD, United Kingdom. Richard.Collins@manchester.ac.uk
Beis
Konstantinos
K
Clarke
Bradley R
BR
Ford
Robert C
RC
Hulley
Martyn
M
Naismith
James H
JH
Whitfield
Chris
C
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002210:Caprylates / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D005466:Fluorocarbons / Q000494:pharmacology; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D008970:Molecular Weight; D009154:Mutation; D009844:Oligosaccharides / Q000737:chemistry; D033903:Periplasmic Proteins / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D025941:Protein Interaction Mapping; D011505:Protein-Tyrosine Kinases / Q000737:chemistry* / Q000378:metabolism; D013045:Species Specificity; D014443:Tyrosine / Q000737:chemistry
D001426:Bacterial Proteins; D002210:Caprylates; D003902:Detergents; D005466:Fluorocarbons; D008565:Membrane Proteins; D009844:Oligosaccharides; D033903:Periplasmic Proteins; C417168:WZC protein, Bacteria; D014443:Tyrosine; C023036:perfluorooctanoic acid; D011505:Protein-Tyrosine Kinases