title: [Hybrid Capture 2 vs HPV Consensus kit: comparison of 2 assays for human papillomavirus detection and typing].
abstract: BACKGROUND
  Many laboratories use the DNA Hybrid Capture 2 HPV-high risk assay (Digene) to detect and type oncogenic HPV. The aim of this work was to compare this assay with a new HPV genotyping assay: HPV Consensus kit (Argène). Actually, this assay is not commercially available.
  
  
  METHODS
  Ninety-four cervical samples were tested with both the routine assay Hybrid Capture 2 and the HPV Consensus kit. Discordant results were analysed by amplification with a nested PCR and sequencing of amplified products.
  
  
  RESULTS
  Only 81 results could be analysed concerning the oncogenic risk. The overall concordance was 92,6%. But we find 13 "generic" results with the HPV Consensus kit, the generic probe including high risk and low risk genotypes.
  
  
  CONCLUSIONS
  HPV Consensus kit results showed a better detection sensitivity for this assay than Hybrid Capture 2 assay. Nevertheless, "generic" results give no information about the oncogenic risk of the HPV detected in a sample.
authors.affiliation: Laboratoire de bactériologie-virologie-hygiène, CHRU Dupuytren, 2 avenue Martin-Luther-King, 87042 Limoges cedex, France. shantz@wanadoo.fr
authors.forename: S
authors.initials: S
authors.lastname: Hantz
authors.forename: H
authors.initials: H
authors.lastname: Caly
authors.forename: E
authors.initials: E
authors.lastname: Decroisette
authors.forename: V
authors.initials: V
authors.lastname: Fermeaux
authors.forename: D
authors.initials: D
authors.lastname: Bakeland
authors.forename: B
authors.initials: B
authors.lastname: Pascal
authors.forename: G
authors.initials: G
authors.lastname: Darreye
authors.forename: C
authors.initials: C
authors.lastname: Dussartre
authors.forename: S
authors.initials: S
authors.lastname: Rogez
authors.forename: Y
authors.initials: Y
authors.lastname: Aubard
authors.forename: F
authors.initials: F
authors.lastname: Denis
authors.forename: S
authors.initials: S
authors.lastname: Alain
mesh_terms: D017931:DNA Primers; D005838:Genotype; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers