Hippocampal progenitor cells express nestin following cerebral ischemia in rats.
This study addresses whether hippocampal progenitor cells express nestin following cerebral ischemia in rats. Cell counts within the hippocampal hilus were significantly greater following severe (eight-vessel occlusion) ischemia than following intermediate (four-vessel occlusion) ischemia (1527+/-87/mm2 vs. 918+/-71/mm2). Bromedeoxyuridine-positive cell counts were significantly higher with severe ischemia than with intermediate ischemia or in sham-operated animals, respectively (368+/-45, 43+/-14 and 7+/-1/mm2). In the eight-vessel occlusion group, 47+/-8/mm2 bromedeoxyuridine-labeled cells expressed nestin, significantly higher than in the four-vessel occlusion group and sham-operated animals (1+/-1 and 1+/-0/mm2, P<0.01 vs. eight-vessel occlusion, respectively). Confocal microscopy verified that a subset of the bromedeoxyuridine-positive cells expressed nestin. In conclusion, severe ischemia elicits nestin expression in hippocampal progenitor cells in rats.
He
Zhen
Z
Department of Pharmacology, Mayo Clinic Jacksonville, Jacksonville, Florida 32224, USA. he.zhen@mayo.edu
Cui
Li
L
Meschia
James F
JF
Dickson
Dennis W
DW
Brott
Thomas G
TG
Simpkins
James W
JW
Day
Arthur L
AL
McKinney
Michael
M
D000704:Analysis of Variance; D000818:Animals; D001157:Arterial Occlusive Diseases / Q000150:complications / Q000378:metabolism; D002545:Brain Ischemia / Q000209:etiology / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D005260:Female; D006624:Hippocampus / Q000166:cytology*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000378:metabolism*
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D001973:Bromodeoxyuridine