title: Hippocampal progenitor cells express nestin following cerebral ischemia in rats.
abstract: This study addresses whether hippocampal progenitor cells express nestin following cerebral ischemia in rats. Cell counts within the hippocampal hilus were significantly greater following severe (eight-vessel occlusion) ischemia than following intermediate (four-vessel occlusion) ischemia (1527+/-87/mm2 vs. 918+/-71/mm2). Bromedeoxyuridine-positive cell counts were significantly higher with severe ischemia than with intermediate ischemia or in sham-operated animals, respectively (368+/-45, 43+/-14 and 7+/-1/mm2). In the eight-vessel occlusion group, 47+/-8/mm2 bromedeoxyuridine-labeled cells expressed nestin, significantly higher than in the four-vessel occlusion group and sham-operated animals (1+/-1 and 1+/-0/mm2, P<0.01 vs. eight-vessel occlusion, respectively). Confocal microscopy verified that a subset of the bromedeoxyuridine-positive cells expressed nestin. In conclusion, severe ischemia elicits nestin expression in hippocampal progenitor cells in rats.
authors.affiliation: Department of Pharmacology, Mayo Clinic Jacksonville, Jacksonville, Florida 32224, USA. he.zhen@mayo.edu
authors.forename: Zhen
authors.initials: Z
authors.lastname: He
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.forename: James F
authors.initials: JF
authors.lastname: Meschia
authors.forename: Dennis W
authors.initials: DW
authors.lastname: Dickson
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Brott
authors.forename: James W
authors.initials: JW
authors.lastname: Simpkins
authors.forename: Arthur L
authors.initials: AL
authors.lastname: Day
authors.forename: Michael
authors.initials: M
authors.lastname: McKinney
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001157:Arterial Occlusive Diseases / Q000150:complications / Q000378:metabolism; D002545:Brain Ischemia / Q000209:etiology / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D005260:Female; D006624:Hippocampus / Q000166:cytology*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D001973:Bromodeoxyuridine