title: Recognition and ubiquitination of Salmonella type III effector SopA by a ubiquitin E3 ligase, HsRMA1.
abstract: Salmonella translocate bacterial effectors into host cells to confer bacterial entry and survival. It is not known how the host cells cope with the influx of these effectors. We report here that the Salmonella effector, SopA, interacts with host HsRMA1, a ubiquitin E3 ligase with a previously unknown function. SopA is ubiquitinated and degraded by the HsRMA1-mediated ubiquitination pathway. A sopA mutant escapes out of the Salmonella-containing vacuoles less frequently to the cytosol than wild type Salmonella in HeLa cells in a HsRMA1-dependent manner. Our data suggest that efficient bacterial escape into the cytosol of epithelial cells requires HsRMA1-mediated SopA ubiquitination and contributes to Salmonella-induced enteropathogenicity.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wendy
authors.initials: W
authors.lastname: Higashide
authors.forename: Shipan
authors.initials: S
authors.lastname: Dai
authors.forename: Debra M
authors.initials: DM
authors.lastname: Sherman
authors.forename: Daoguo
authors.initials: D
authors.lastname: Zhou
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism* / Q000502:physiology*; D004926:Escherichia coli / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D034741:RNA, Small Interfering / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D012475:Salmonella / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014162:Transfection; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin / Q000737:chemistry; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D025801:Ubiquitin; C453342:rma protein, Salmonella; D044767:Ubiquitin-Protein Ligases; C060971:SopA protein, Bacteria