title: Localization, specific activity, and molecular forms of acetylcholinesterase in developmental stages of the cestode Mesocestoides corti.
abstract: The nervous system of flatworms is quite simple although there is increasing evidence indicating that it is chemically complex. Studies of the nervous system in these animals have only been performed in the larval stage or in the adult worms, which are easy to obtain in nature, while the description of the nervous system in developing stages of these organisms is missing. Mesocestoides corti is a parasitic platyhelminth whose larvae can be induced in vitro to develop to adult, sexually mature worms, opening the possibility of studying the nervous system of a flatworm in different stages of development. Here, we describe the presence, activity, location, and molecular forms of acetylcholinesterase (AChE) in different stages of development of M. corti, from the larvae to adult forms of this endoparasite, obtained in in vitro cultures after induction of the larval stage with trypsin. Our results point to AChE as a molecular marker of the nervous system in platyhelminthes. The change in molecular forms of this enzyme and the increase in its activity during development from larvae to adult worm may reflect the presence of a more complex nervous system, necessary to adjust and coordinate the movement of a much bigger structure. A relationship between the development of the reproductive apparatus in segmented and adult worms with a more complex nervous system in these stages is also apparent. Finally, our study opens the possibility of applying anti-AChE as more effective therapeutic strategies against cestode parasites.
authors.affiliation: Departamento de Estomatología, Facultad de Ciencias de la Salud, Universidad de Talca, Talca, Chile.
authors.forename: U
authors.initials: U
authors.lastname: Kemmerling
authors.forename: G
authors.initials: G
authors.lastname: Cabrera
authors.forename: E O
authors.initials: EO
authors.lastname: Campos
authors.forename: N C
authors.initials: NC
authors.lastname: Inestrosa
authors.forename: N
authors.initials: N
authors.lastname: Galanti
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D005260:Female; D008018:Life Cycle Stages / Q000187:drug effects / Q000502:physiology*; D008645:Mesocestoides / Q000254:growth & development* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000110:Acetylcholinesterase; D014357:Trypsin