Cation fluxes cause plasma membrane depolarization involved in beta-glucan elicitor-signaling in soybean roots.
Inducible and specific ion fluxes on plasma membranes represent very early events during elicitation of plant cells. The hierarchy of such ion fluxes involved is still unknown. The effect of Phytophthora sojae-derived beta-glucan elicitors on the plasma membrane potential as well as on surface K+, Ca2+, and H+ fluxes has been investigated on soybean roots using ion-selective microelectrodes. Beta-Glucans with different degrees of polymerization transiently depolarized the plasma membrane. The elicitor concentration necessary for half-maximal depolarization closely resembled the corresponding binding affinities of soybean root membranes toward the respective beta-glucans. Upon repeated elicitor treatment, the root cells responded partially refractory, suggesting a complex responsiveness of the system. Within the root hair space, characteristic decreasing K(+)- and Ca(2+)-free concentrations were induced by the elicitors, probably causing depolarization through the influx of positive charges. Whereas K+ fluxes were inverted after passing the K+ equilibrium (Nernst-) potential, Ca2+ influx continued. No anion fluxes sufficient to account for charge compensation were observed under the same experimental conditions. K+ and Ca2+ fluxes as well as depolarization were inhibited by 100 microM or less of the Ca2+ antagonist La3+. Contrasting other systems, in soybean the main cause for elicitor-induced plasma membrane depolarization is the activation of cation instead of anion fluxes.
Mithöfer
Axel
A
Max-Planck-Institut für Chemische Okologie, Bioorganische Chemie, Hans-Knöll-Str. 8, D-07745 Jena, Germany. amithoefer@ice.mpg.de
Ebel
Jürgen
J
Felle
Hubert H
HH
D002412:Cations / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects; D007811:Lanthanum / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D010838:Phytophthora / Q000737:chemistry / Q000472:pathogenicity; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013025:Glycine max / Q000187:drug effects* / Q000378:metabolism* / Q000382:microbiology; D047071:beta-Glucans / Q000302:isolation & purification / Q000494:pharmacology*
D002412:Cations; D047071:beta-Glucans; D007811:Lanthanum