title: Desferrioxamine E produced by Streptomyces griseus stimulates growth and development of Streptomyces tanashiensis.
abstract: The authors previously reported that interspecific stimulatory events between Streptomyces species for antibiotic production and/or morphological differentiation mediated by putative diffusible metabolites take place at a high frequency. This paper reports the isolation and characterization of a substance produced by Streptomyces griseus that stimulates the growth and development of Streptomyces tanashiensis. The substance was purified from the culture supernatant of S. griseus by using anion-exchange chromatography, gel filtration chromatography and reverse-phase HPLC. FAB-MS and NMR analyses of the purified preparation indicated the substance to be desferrioxamine E (synonym: nocardamine), a siderophore that is widely produced by Streptomyces species and related organisms. Similar stimulatory effects on the growth and development of S. tanashiensis were exerted by desferrioxamine E produced by another actinomycete strain, but not by other siderophores tested, including ferrichrome and nocobactin and free ferric ion. An exogenous supply of desferrioxamine E stimulated secondary metabolite formation and/or morphological differentiation in various actinomycete strains. Disruption of the desferrioxamine biosynthesis gene cluster in Streptomyces coelicolor A3(2) abolished the production of desferrioxamine E and the activity to stimulate the growth and differentiation of S. tanashiensis. The S. coelicolor mutant showed impaired growth and development on Bennett's/glucose agar medium, but it was rescued by the exogenous supply of desferrioxamine E. These results indicate that desferrioxamines play an important role in streptomycete physiology. Similar to several pathogenic bacteria and fungi, S. tanashiensis may be defective in the production of siderophores; however, it can utilize the siderophores excreted by other organisms.
authors.affiliation: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yamanaka
authors.affiliation: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Oikawa
authors.affiliation: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan.
authors.forename: Hiro-Omi
authors.initials: HO
authors.lastname: Ogawa
authors.affiliation: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan.
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Hosono
authors.affiliation: Department of Agriculture, Junior College, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan.
authors.forename: Fumie
authors.initials: F
authors.lastname: Shinmachi
authors.affiliation: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Takano
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Sakuda
authors.affiliation: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan.
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Beppu
authors.affiliation: Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Ueda
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003676:Deferoxamine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013302:Streptomyces / Q000187:drug effects; D013305:Streptomyces griseus / Q000737:chemistry*; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D015534:Trans-Activators; D003676:Deferoxamine