title: Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125.
abstract: A considerable fraction of life develops in the sea at temperatures lower than 15 degrees C. Little is known about the adaptive features selected under those conditions. We present the analysis of the genome sequence of the fast growing Antarctica bacterium Pseudoalteromonas haloplanktis TAC125. We find that it copes with the increased solubility of oxygen at low temperature by multiplying dioxygen scavenging while deleting whole pathways producing reactive oxygen species. Dioxygen-consuming lipid desaturases achieve both protection against oxygen and synthesis of lipids making the membrane fluid. A remarkable strategy for avoidance of reactive oxygen species generation is developed by P. haloplanktis, with elimination of the ubiquitous molybdopterin-dependent metabolism. The P. haloplanktis proteome reveals a concerted amino acid usage bias specific to psychrophiles, consistently appearing apt to accommodate asparagine, a residue prone to make proteins age. Adding to its originality, P. haloplanktis further differs from its marine counterparts with recruitment of a plasmid origin of replication for its second chromosome.
authors.affiliation: Genoscope, CNRS-UMR 8030, Atelier de Génomique Comparative, 91006 Evry Cedex, France.
authors.forename: Claudine
authors.initials: C
authors.lastname: Médigue
authors.forename: Evelyne
authors.initials: E
authors.lastname: Krin
authors.forename: Géraldine
authors.initials: G
authors.lastname: Pascal
authors.forename: Valérie
authors.initials: V
authors.lastname: Barbe
authors.forename: Andreas
authors.initials: A
authors.lastname: Bernsel
authors.forename: Philippe N
authors.initials: PN
authors.lastname: Bertin
authors.forename: Frankie
authors.initials: F
authors.lastname: Cheung
authors.forename: Stéphane
authors.initials: S
authors.lastname: Cruveiller
authors.forename: Salvino
authors.initials: S
authors.lastname: D'Amico
authors.forename: Angela
authors.initials: A
authors.lastname: Duilio
authors.forename: Gang
authors.initials: G
authors.lastname: Fang
authors.forename: Georges
authors.initials: G
authors.lastname: Feller
authors.forename: Christine
authors.initials: C
authors.lastname: Ho
authors.forename: Sophie
authors.initials: S
authors.lastname: Mangenot
authors.forename: Gennaro
authors.initials: G
authors.lastname: Marino
authors.forename: Johan
authors.initials: J
authors.lastname: Nilsson
authors.forename: Ermenegilda
authors.initials: E
authors.lastname: Parrilli
authors.forename: Eduardo P C
authors.initials: EP
authors.lastname: Rocha
authors.forename: Zoé
authors.initials: Z
authors.lastname: Rouy
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sekowska
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Tutino
authors.forename: David
authors.initials: D
authors.lastname: Vallenet
authors.forename: Gunnar
authors.initials: G
authors.lastname: von Heijne
authors.forename: Antoine
authors.initials: A
authors.lastname: Danchin
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D003080:Cold Temperature; D016166:Free Radical Scavengers; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D020543:Proteome; D031161:Pseudoalteromonas / Q000235:genetics* / Q000502:physiology*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017382:Reactive Oxygen Species
chemical_list: D016166:Free Radical Scavengers; D020543:Proteome; D012329:RNA, Bacterial; D017382:Reactive Oxygen Species