title: Characteristics of manganese (Mn) transport in rat brain endothelial (RBE4) cells, an in vitro model of the blood-brain barrier.
abstract: Manganese (Mn), an essential elemental nutrient, is known to be neurotoxic at high occupational levels. We examined the transport of Mn across a monolayer of rat brain endothelial cell (RBE4) to evaluate whether an electromotive permeability mechanism is responsible for Mn transport across the blood-brain barrier (BBB). The (54)Mn(2+) apparent permeability and flux showed significant temperature-, energy- and pH-dependence, as well as partial sodium-dependence. Additionally, iron (Fe)-rich and Fe-deficient media significantly increased the apparent permeability of (54)Mn(2+). Finally, Mn flux and permeability decreased when RBE4 cells were grown in astrocyte-conditioned media (ACM), compared to standard alpha-media. These data reinforce observations that transport of Mn across the BBB occurs in part through active transport process.
authors.affiliation: Department of Pediatrics, B-3307 Medical Center North, Vanderbilt University School of Medicine, Nashville, TN 37232-2495, USA.
authors.forename: Vanessa A
authors.initials: VA
authors.lastname: Fitsanakis
authors.forename: Greg
authors.initials: G
authors.lastname: Piccola
authors.forename: Judy L
authors.initials: JL
authors.lastname: Aschner
authors.forename: Michael
authors.initials: M
authors.lastname: Aschner
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001812:Blood-Brain Barrier / Q000378:metabolism*; D002199:Capillary Permeability; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D042783:Endothelial Cells / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism; D008345:Manganese / Q000378:metabolism*; D051381:Rats; D012402:Rotenone / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D017077:Culture Media, Conditioned; D014475:Uncoupling Agents; D012402:Rotenone; D008345:Manganese; D012964:Sodium; D007501:Iron