title: Effects of caffeic acid phenethyl ester on endotoxin-induced uveitis in rats.
abstract: OBJECTIVE
  Caffeic acid phenethyl ester (CAPE) has antimicrobial, anti-inflammatory, antioxidant, immunomodulatory, and carcinostatic properties. In this study, the efficacy of CAPE in endotoxin-induced uveitis (EIU) in rats is investigated.
  
  
  METHODS
  EIU was induced by a footpad injection of lipopolysaccharide (LPS). In the treatment group, 10 micromol/kg CAPE was injected intraperitoneally immediately after LPS injection. At 24 hr after LPS injection, the number of infiltrating cells, protein concentration, and levels of myeloperoxidase (MPO) in aqueous humor; malondialdehyde (MDA), MPO, and total antioxidant levels in serum were determined. Eyes were enucleated for histopathologic evaluation, and, counting inflammatory cells in iris-ciliary body (ICB), the efficacy of treatment was determined.
  
  
  RESULTS
  CAPE significantly suppressed LPS-induced increase in the number of inflammatory cells (p = 0.0001), protein concentration (p = 0.0001), and MPO levels (p = 0.0001) in aqueous humor as well as MDA (p = 0.001) and MPO (p = 0.0001) levels in serum. Histopathologic evaluation of ICB showed significant reduction in the inflammatory cell counts in the treatment group (p = 0.0001).
  
  
  CONCLUSIONS
  CAPE was found efficient in suppressing inflammation and ocular tissue damage induced by LPS in rats.
authors.affiliation: Mersin University School of Medicine, Ophthalmology Department, Mersin, Turkey. aycayilmaz@mersin.edu.tr
authors.forename: Ayça
authors.initials: A
authors.lastname: Yilmaz
authors.forename: Ozlem
authors.initials: O
authors.lastname: Yildirim
authors.forename: Lülüfer
authors.initials: L
authors.lastname: Tamer
authors.forename: Ozay
authors.initials: O
authors.lastname: Oz
authors.forename: Leyla
authors.initials: L
authors.lastname: Cinel
authors.forename: Halil
authors.initials: H
authors.lastname: Vatansever
authors.forename: Ulaş
authors.initials: U
authors.lastname: Değirmenci
authors.forename: Arzu
authors.initials: A
authors.lastname: Kanik
authors.forename: Uğur
authors.initials: U
authors.lastname: Atik
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D001082:Aqueous Humor / Q000166:cytology / Q000378:metabolism; D002109:Caffeic Acids / Q000627:therapeutic use*; D002924:Ciliary Body / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007498:Iris / Q000378:metabolism / Q000473:pathology; D008070:Lipopolysaccharides; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium; D014605:Uveitis / Q000139:chemically induced / Q000188:drug therapy*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002109:Caffeic Acids; D005136:Eye Proteins; D008070:Lipopolysaccharides; D009195:Peroxidase; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol