title: Identification of Actinobacillus suis genes essential for the colonization of the upper respiratory tract of swine.
abstract: Actinobacillus suis has emerged as an important opportunistic pathogen of high-health-status swine. A colonization challenge method was developed, and using PCR-based signature-tagged transposon mutagenesis, 13 genes belonging to 9 different functional classes were identified that were necessary for A. suis colonization of the upper respiratory tract of swine.
authors.affiliation: University of Guelph, Ontario, Canada.
authors.forename: Shivani
authors.initials: S
authors.lastname: Ojha
authors.forename: Marc
authors.initials: M
authors.lastname: Sirois
authors.forename: Janet I
authors.initials: JI
authors.lastname: Macinnes
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary*; D044132:Actinobacillus suis / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D000818:Animals; D004251:DNA Transposable Elements; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D016254:Mutagenesis, Insertional; D009305:Nasopharynx / Q000382:microbiology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014774:Virulence / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements