Effect of Cd on GSH and GSH-related enzymes of Chlamydomonas sp. ICE-L existing in Antarctic ice.
Glutathione (GSH) and GSH-related enzymes play a great role in protecting organisms from oxidative damage. The GSH level and GSH-related enzymes activities were investigated as well as the growth yield and malonyldialdehyde (MDA) content in the Antarctic ice microalga Chlamydomonas sp. ICE-L exposure to the different cadmium concentration in this paper. The results showed that the higher concentration Cd inhibited the growth of ICE-L significantly and Cd would induce formation of MDA. At the same time, it is clear that GSH level, glutathione peroxidases (GPx) activity and glutathione S-transferases (GST), activity were higher in ICE-L exposed to Cd than the control. But GR activity dropped notably when ICE-L were cultured in the medium containing Cd. Increase of GSH level, GPx and GST activities acclimate to oxidative stress induced by Cd and protect Antarctic ice microalga Chlamydomonas sp. ICE-L from toxicity caused by Cd exposure. These parameters may be used to assess the biological impact of Cd in the Antarctic pole region environment.
Ding
Yu
Y
Marine Life College, Ocean University of China, Qingdao 266003, China. dingyuddd@163.com
Miao
Jin-Lai
JL
Li
Guang-You
GY
Wang
Quan-Fu
QF
Kan
Guang-Feng
GF
Wang
Guo-Dong
GD
D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D002696:Chlamydomonas / Q000201:enzymology* / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D007053:Ice
D007053:Ice; D002104:Cadmium; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione