Genetic manipulation of proline accumulation influences the concentrations of other amino acids in soybean subjected to simultaneous drought and heat stress.
The effect of simultaneous drought and heat stress on free amino acid levels was compared in wild type and transgenic soybean (Glycine max (L.) Merr cv Ibis) plants transformed with the cDNA coding for the last enzyme of Pro biosynthesis, l-Delta(1)-pyrroline-5-carboxylate reductase (EC 1.5.1.2), in sense and antisense directions. The most rapid increase in Pro content was found in the sense transformants that exhibited the least water loss, while the slowest elevation of Pro levels was detected in the antisense transformants that exhibited the greatest water loss during stress. Correspondingly, the level of the Pro precursors Glu and Arg was higher in sense transformants and lower in antisense ones compared to the wild type plants during the initial part of the stress. Interestingly, genetic manipulation of Pro levels also affected the stress-induced changes in the concentration of several other amino acids, which indicates the coordinated regulation of their metabolic pathways.
Simon-Sarkadi
Livia
L
Department of Biochemistry and Food Technology, Budapest University of Technology and Economics, Hungary. sarkadi@mail.bme.hu
Kocsy
Gábor
G
Várhegyi
Agnes
A
Galiba
Gábor
G
de Ronde
Jacoba A
JA
D000596:Amino Acids / Q000032:analysis*; D006358:Hot Temperature; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism*; D011392:Proline / Q000096:biosynthesis*; D011762:Pyrroline Carboxylate Reductases / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014867:Water; D000097502:delta-1-Pyrroline-5-Carboxylate Reductase
D000596:Amino Acids; D011392:Proline; D011762:Pyrroline Carboxylate Reductases; D014867:Water; D000097502:delta-1-Pyrroline-5-Carboxylate Reductase