title: Identification of nitrated proteins in the normal rat brain using a proteomics approach.
abstract: BACKGROUND
  The nitration of tyrosine has been suggested to play a role in the pathogenesis of neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD) and Alzheimer's disease (AD).
  
  
  METHODS
  In the present study, we identified four targets of protein nitration, T-complex polypeptide 1 alpha subunit (TCP-1), neurofilament L (NFL), glial fibrillary acidic protein (GFAP) and clathrin heavy chain (CHC), in the normal rat cortex using a proteomics approach.
  
  
  CONCLUSIONS
  There have been no reports on these proteins being identified by proteomics as nitrated forms in the brain. For further study, we have to investigate alterations in these nitrated proteins during aging and in neurodegenerative disorders.
authors.affiliation: Department of Neurology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan. suzuyoko@showa.gunma-u.ac.jp
authors.forename: Yoko
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Makoto
authors.initials: M
authors.lastname: Tanaka
authors.forename: Makoto
authors.initials: M
authors.lastname: Sohmiya
authors.forename: Sachiyo
authors.initials: S
authors.lastname: Ichinose
authors.forename: Akira
authors.initials: A
authors.lastname: Omori
authors.forename: Koichi
authors.initials: K
authors.lastname: Okamoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000378:metabolism*; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins / Q000302:isolation & purification / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D033922:Clathrin Heavy Chains / Q000302:isolation & purification / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005904:Glial Fibrillary Acidic Protein / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D016900:Neurofilament Proteins / Q000302:isolation & purification / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005904:Glial Fibrillary Acidic Protein; D016900:Neurofilament Proteins; D009566:Nitrates; C044882:neurofilament protein L; D033922:Clathrin Heavy Chains; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins