title: Staphylococcus simiae sp. nov., isolated from South American squirrel monkeys.
abstract: Eight coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococcal strains were isolated from the gastrointestinal tracts of South American squirrel monkeys (Saimiri sciureus L.). These strains were differentiated from known staphylococcal species on the basis of 16S rRNA gene and hsp60 gene sequencing, and from the most closely related species by using DNA-DNA hybridization, ribotyping, whole-cell protein profiles and biotyping. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that these strains are members of the Staphylococcus aureus species group (99% similarity) but are biochemically similar to Staphylococcus piscifermentans, from which they can be phenotypically distinguished by resistance to polymyxin B, acid production from D-mannitol, the inability to hydrolyse aesculin and DNA and the absence of alpha-glucosidase. On the basis of these analyses, a novel species of the genus Staphylococcus is described, for which the name Staphylococcus simiae sp. nov. is proposed, with CCM 7213(T) (=LMG 22723(T)) as the type strain.
authors.affiliation: Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic.
authors.forename: Roman
authors.initials: R
authors.lastname: Pantůček
authors.affiliation: Czech Collection of Microorganisms, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Ivo
authors.initials: I
authors.lastname: Sedláček
authors.affiliation: Reference Laboratory for Staphylococci, National Institute of Public Health, Šrobárova 48, 100 42 Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Petráš
authors.affiliation: Institute of Microbiology, Faculty of Medicine, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Koukalová
authors.affiliation: Czech Collection of Microorganisms, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Švec
authors.affiliation: Czech Collection of Microorganisms, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Štětina
authors.affiliation: BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Vancanneyt
authors.affiliation: Zoo Olomouc, Darwinova 29, 772 00 Olomouc, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Chrastinová
authors.affiliation: Zoo Olomouc, Darwinova 29, 772 00 Olomouc, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Vokurková
authors.affiliation: Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic.
authors.forename: Vladislava
authors.initials: V
authors.lastname: Růžičková
authors.affiliation: Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Doškař
authors.affiliation: BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
authors.affiliation: Institute of Microbiology, Faculty of Medicine, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Hájek
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D041981:Gastrointestinal Tract / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D012453:Saimiri / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013020:South America; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S