Cytokine responses in immunized and non-immunized calves after Ostertagia ostertagi infection.
The objective of this study was to evaluate abomasal cytokine responses in helminth-naive calves and calves vaccinated with protective antigen fractions from Ostertagia ostertagi after an experimental challenge infection with infective third stage (L3) larvae. Abomasal lymph nodes and/or abomasal mucosa were collected and messenger RNA for the Th1 cytokines (IFN-gamma, IL-2, IL-12 p40 subunit), the Th2 cytokines (IL-4, IL-5, IL-6, IL-10, IL-13, IL-15) and the Th3/Tr cytokine TGF-beta was quantified by real-time RT-PCR. Vaccination had no effect on cytokine profiles in either the abomasal lymph nodes or the abomasal mucosa. However, following infection all calves showed a significant decrease in the Th1 cytokines, IFN-gamma and IL-12 p40, and a significant increase in the Th2 cytokines, IL-4, IL-5, IL-10 and IL-13 in the lymph nodes, compared to non-infected calves. No correlation between the Th2 response and protection induced by vaccination could be demonstrated. In contrast, a Th2 pattern was not observed in the mucosa of the infected calves, which exhibited an increase in IFN-gamma as well as in the Th2 cytokines IL-4, IL-5 and IL-10 mRNA. No significant association was observed in the abomasal mucosa between any examined cytokine mRNA level and immune effector responses such as parasite-specific antibodies or the number of mucosal mast cells or eosinophils.
Claerebout
E
E
Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. edwin.claerebout@ugent.be
Vercauteren
I
I
Geldhof
P
P
Olbrechts
A
A
Zarlenga
D S
DS
Goddeeris
B M
BM
Vercruysse
J
J
D000018:Abomasum / Q000276:immunology; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D002452:Cell Count; D016207:Cytokines / Q000032:analysis* / Q000235:genetics; D004804:Eosinophils; D020869:Gene Expression Profiling; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D008407:Mast Cells; D009092:Mucous Membrane / Q000276:immunology; D010028:Ostertagia / Q000276:immunology*; D010029:Ostertagiasis / Q000276:immunology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000909:Antibodies, Helminth; D016207:Cytokines; D012333:RNA, Messenger; D014612:Vaccines