title: A FancD2-monoubiquitin fusion reveals hidden functions of Fanconi anemia core complex in DNA repair.
abstract: In DNA damage responses, the Fanconi anemia (FA) protein, FancD2, is targeted to chromatin and forms nuclear foci following its monoubiquitination, a process likely catalyzed by the FA core complex. Here, we show that a chicken FancD2-ubiquitin fusion protein, carrying a Lys-Arg substitution removing the natural monoubiquitination site (D2KR-Ub), could reverse cisplatin hypersensitivity and localize to chromatin in FANCD2-deficient DT40 cells. Importantly, the chromatin targeting was dependent on three core complex components as well as the hydrophobic surface of ubiquitin that may direct protein-protein interactions. Furthermore, a constitutively chromatin bound fusion of D2KR-histone H2B could complement cisplatin sensitivity in FANCD2- but not FANCC-, FANCG-, or FANCL-deficient cells. Thus these core complex components have an additional function in the DNA repair, which is independent of the monoubiquitination and chromatin targeting of FancD2. These results define functional consequences of FancD2 monoubiquitination and reveal previously hidden functions for the FA protein core complex.
authors.affiliation: Department of Immunology and Molecular Genetics, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Matsushita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kitao
authors.forename: Masamichi
authors.initials: M
authors.lastname: Ishiai
authors.forename: Naoki
authors.initials: N
authors.lastname: Nagashima
authors.forename: Seiki
authors.initials: S
authors.lastname: Hirano
authors.forename: Katsuya
authors.initials: K
authors.lastname: Okawa
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Ohta
authors.forename: David S
authors.initials: DS
authors.lastname: Yu
authors.forename: Peter J
authors.initials: PJ
authors.lastname: McHugh
authors.forename: Ian D
authors.initials: ID
authors.lastname: Hickson
authors.forename: Ashok R
authors.initials: AR
authors.lastname: Venkitaraman
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kurumizaka
authors.forename: Minoru
authors.initials: M
authors.lastname: Takata
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D002843:Chromatin / Q000378:metabolism; D002869:Chromosome Aberrations; D004260:DNA Repair; D005816:Genetic Complementation Test; D006657:Histones / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D006657:Histones; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; D025801:Ubiquitin