title: The targeted disruption of the MYPT1 gene results in embryonic lethality.
abstract: Myosin phosphatase (MP) is a major phosphatase responsible for the dephosphorylation of the regulatory light chain of myosin II. MYPT1, a target subunit of smooth and nonmuscle MP, is responsible for activation and regulation of MP. To identity the physiological roles of MP, we have generated MYPT1-deficient mice by gene targeting. The heterozygous mice showed no changes in expression levels of MYPT1 and no distinct phenotype compared to wild-type mice was observed. None of the F2 mice were homozygous for the MYPT1 deletion, indicating that the targeted disruption of the MYPT1 gene resulted in embryonic lethality. The point of embryonic lethality is before 7.5 dpc. These findings indicate that MYPT1 is essential for mouse embryogenesis.
authors.affiliation: The First Department of Internal Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Okamoto
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ito
authors.forename: Noboru
authors.initials: N
authors.lastname: Suzuki
authors.forename: Mariko
authors.initials: M
authors.lastname: Kongo
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Moriki
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Saito
authors.forename: Hideki
authors.initials: H
authors.lastname: Tsumura
authors.forename: Kyoko
authors.initials: K
authors.lastname: Imanaka-Yoshida
authors.forename: Kazushi
authors.initials: K
authors.lastname: Kimura
authors.forename: Akira
authors.initials: A
authors.lastname: Mizoguchi
authors.forename: David J
authors.initials: DJ
authors.lastname: Hartshorne
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakano
mesh_terms: D000818:Animals; D005260:Female; D018390:Gene Targeting; D005804:Genes, Lethal; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D043263:Myosin-Light-Chain Phosphatase / Q000235:genetics* / Q000378:metabolism
chemical_list: D043263:Myosin-Light-Chain Phosphatase