An optical glucose biosensor based on glucose oxidase immobilized on a swim bladder membrane.
An optical glucose biosensor using a swim bladder membrane as an enzyme immobilization platform and an oxygen-sensitive membrane as an optical oxygen transducer has been developed. During the enzymatic reaction, glucose is oxidized by glucose oxidase with a concomitant consumption of dissolved oxygen resulting in an increase in the fluorescence intensity of the optical oxygen transducer. The fluorescence intensity is directly related to the glucose concentration. The effects of pH, temperature, buffer concentration, and selectivity have been studied in detail. The immobilized enzyme retained 80% of its initial activity after being kept for more than 10 months at 4 degrees C. The glucose biosensor has been successfully applied to the determination of glucose content in human blood serum and urine samples.
Zhou
Zaide
Z
College of Chemistry, Sichuan University, Chengdu, 610065, P.R. China.
Qiao
Lina
L
Zhang
Peng
P
Xiao
Dan
D
Choi
Martin M F
MM
D000400:Air Sacs / Q000166:cytology; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis; D002347:Carps; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008566:Membranes / Q000378:metabolism*; D055095:Optics and Photonics; D014556:Urine / Q000737:chemistry
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D005947:Glucose