Coating of poly(dimethylsiloxane) with n-dodecyl-beta-D-maltoside to minimize nonspecific protein adsorption.
Poly(dimethylsiloxane)(PDMS) surface is coated with n-dodecyl-beta-D-maltoside, which reduces the nonspecifically adsorbed protein on the PDMS surface to the single molecule level.
Huang
Bo
B
Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA.
Wu
Hongkai
H
Kim
Samuel
S
Zare
Richard N
RN
D000327:Adsorption; D000818:Animals; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology*; D051379:Mice; D008856:Microscopy, Fluorescence; D040081:Protein Array Analysis / Q000295:instrumentation*; D012828:Silicones / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties
D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D005960:Glucosides; D012828:Silicones; C013830:baysilon; C040358:dodecyl maltoside; D019809:Streptavidin