High-performance liquid chromatography-tandem mass spectrometry validation of medroxyprogesterone acetate in products of pork origin and serum.
Different extraction and purification methods are described here to determine medroxyprogesterone acetate (MPA) in pork meat and serum. Spiked samples are investigated over the concentration range of MPA 0.5-20 ng/g. Pork meat tissues are subjected to extraction using organic solvent, and pork serum is simply diluted with acetate buffer. Clean-up is performed using solid-phase extraction on a C18 cartridge, and MPA is eluted with ethanol. Aliquots are injected into a high-performance liquid chromatography-mass spectrometry system. MPA content is determined on the basis of m/z 387-327 and 387-123 transitions.
Giannetti
Luigi
L
Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Via Appia Nuova, 1411- Rome, Italy.
Barchi
Daniela
D
Fiorucci
Fulvia
F
Gennuso
Elisa
E
Sanna
Patrizia
P
Pallagrosi
Marco
M
Neri
Bruno
B
D045930:Anabolic Agents / Q000032:analysis* / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat / Q000032:analysis*; D017258:Medroxyprogesterone Acetate / Q000032:analysis* / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
D045930:Anabolic Agents; D017258:Medroxyprogesterone Acetate