Mouse disabled 2 interacting protein 2 functions as a transcriptional repressor through direct binding onto its own promoter.
The mDaIP2 protein is a mouse orthologue of human Nostrin (a regulator of eNos). The absence of eNos activity in RA-treated F9 cell implies that the protein plays somehow different role from Nostrin. In this experiment, this protein has been shown to repress the expression of its own gene, via a feedback mechanism which involves binding to the promoter region. Data from cotransfection, DNAP, mDaIP2-silenced F9 cell, and EMSA analyses clearly support the repression activity and direct binding of the protein to the promoter region. The protein contains N-terminal FCH domain and C-terminal SH3 domain. The SH3 domain is known to interact with the proline-rich domain of mDab2, while even no function has been reported about the FCH domain. Here, we report a novel function of mDaIP2 as a transcriptional repressor and suggest the possible association of the FCH domain with transcriptional regulation.
Kim
Hyung-Wook
HW
School of Life Sciences and Biotechnology, Korea University, 5-ka, Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
Choi
Young-Joon
YJ
Kim
Jung-Ah
JA
Bae
Seong-Ho
SH
Kim
Kyoung-Rae
KR
Park
Sung-Soo
SS
D024506:5' Flanking Region; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001665:Binding Sites; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation; D051379:Mice; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; C495611:Nostrin protein, mouse; D012097:Repressor Proteins; D019001:Nitric Oxide Synthase