High rate of aneuploidy in luteinized granulosa cells obtained from follicular fluid in women who underwent controlled ovarian hyperstimulation.
We performed a prospective study to detect the cell cycle changes in luteinized granulosa cells in patients undergoing controlled ovarian stimulation with GnRH analog and gonadotropin therapy. Aneuploid DNA content was observed in 37 of 70 women (52.9%), and multiple logistic regression analysis revealed that only the dose of gonadotropins used significantly predicted the ploidy status of granulosa cells, which indicates that ovarian hyperstimulation in IVF treatment might be detrimental for the DNA content of granulosa cells.
Kaleli
Semih
S
Department of Obstetrics and Gynecology, Istanbul University, Cerrahpaşa School of Medicine, Istanbul, Turkey. semihkaleli@yahoo.com
Yanikkaya-Demirel
Gulderen
G
Erel
C Tamer
CT
Senturk
Levent M
LM
Topçuoğlu
Ata
A
Irez
Tulay
T
D000782:Aneuploidy; D005260:Female; D015571:Follicular Fluid / Q000166:cytology / Q000502:physiology*; D006107:Granulosa Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D034461:Luteinization / Q000502:physiology*; D016471:Ovarian Hyperstimulation Syndrome / Q000139:chemically induced / Q000503:physiopathology*; D010062:Ovulation Induction / Q000009:adverse effects* / Q000706:statistics & numerical data; D011446:Prospective Studies