Effect of glyphosate herbicide on acetylcholinesterase activity and metabolic and hematological parameters in piava (Leporinus obtusidens).
In this study, teleostean fish Leporinus obtusidens (piava) were exposed to different concentrations of Roundup, a glyphosate (acid equivalent) herbicide: 0 (control), 3, 6, 10, and 20 mg/L for 96 h (short-term). Acetylcholinesterase (AChE) activity was verified in brain and muscle tissues. Metabolic parameters in the liver and muscle (lactate, glycogen, glucose, protein, and ammonia), as well as some hematological parameters, were determined. Unexposed fish exhibited significantly higher brain AChE activity when compared to that of the muscle (P<0.05) (13.8+/-0.76 and 6.1+/-1.31 micromol/min/g protein, respectively). Results indicated that AChE activity significantly decreased in the brain of fish exposed to all glyphosate concentrations tested, but in the muscle this parameter was not altered. In addition, fish exposed to all glyphosate concentrations showed a significant increase in hepatic glycogen and glucose, but a significant reduction in muscle glycogen and glucose. Lactate and protein of fish exposed to all glyphosate concentrations presented a significant decrease in the liver, but did not change significantly in the muscle. Levels of ammonia in both tissues increase in fish at all glyphosate concentrations. Exposure to this herbicide produced a decrease in all hematological parameters tested. These results indicate that AChE activity as well as metabolic and hematological parameters may be good early indicators of herbicide contamination in L. obtusidens.
Glusczak
Lissandra
L
Laboratório de Bioquímica Adaptativa, Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
dos Santos Miron
Denise
D
Crestani
Márcia
M
Braga da Fonseca
Milene
M
de Araújo Pedron
Fábio
F
Duarte
Marta Frescura
MF
Vieira
Vânia Lúcia Pimentel
VL
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001772:Blood Cell Count; D001798:Blood Proteins / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D005399:Fishes; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000097797:Glyphosate
D000110:Acetylcholinesterase; D001798:Blood Proteins; D005998:Glycine; D006540:Herbicides; D014874:Water Pollutants, Chemical; D000097797:Glyphosate