Core-shell CdS/Cd(OH)2 quantum dots: synthesis and bioconjugation to target red cells antigens.
We report a new and efficient methodology of labelling red blood cells, in order to investigate the expression of anti-A antigen, employing luminescent semiconductor nanocrystals. Highly luminescent and stable core-shell cadmium sulphide/cadmium hydroxide [CdS/CdS(OH)2] colloidal particles were obtained in the nanometre size range. The surface of these particles was characterized by using a monoclonal anti-A antibody via a one-step glutaraldehyde cross-linking procedure, followed by conjugation of the particles to red cells of blood groups A+, and O+. Laser scanning confocal microscopy images indicated that after conjugation for 30 min, A+ and erythrocytes presented different patterns of dual bright emission whereas the O+ group cells showed no emission. We suggest that this labelling procedure may be applied as a quantitative tool to investigate the distribution and expression of alloantigen in red blood cells.
de Farias
P M Albuquerque
PM
Departamento de Biofísica e Radiobiologia - CCB, Federal University of Pernambuco, Cid. Universitária CEP 50670-901 Recife-PE, Brazil.
Santos
B Saegesser
BS
de Menezes
F Duarte
FD
Ferreira
R de Carvalho
Rde C
Barjas-Castro
M de Lourdes
Mde L
Castro
V
V
Lima
P R Moura
PR
Fontes
A
A
Cesar
C L
CL
D000497:Alloys; D000911:Antibodies, Monoclonal / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000032:analysis; D002104:Cadmium / Q000737:chemistry; D002863:Chromogenic Compounds / Q000138:chemical synthesis* / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D036103:Nanotechnology / Q000379:methods*; D045663:Quantum Dots
D000497:Alloys; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002863:Chromogenic Compounds; D002104:Cadmium