Appearance of Salmonella enterica isolates producing plasmid-mediated AmpC beta-lactamase, CMY-2, in South Korea.
Of 176 Salmonella isolates isolated from 2000 to 2003 in a Korean university hospital, 2 cefoxitin-resistant isolates of Salmonella enterica serovar Rissen were found in 2002. Both isolates were resistant to multiple beta-lactam antibiotics, including ampicillin, amoxicillin-clavulanate, cefoxitin, cefotaxime, and ceftazidime. Cell sonicates of the both clinical isolates and their transconjugants had beta-lactamase band of approximate isoelectric point of >8.6. The resistance to cefoxitin was transferred by conjugation to the recipient Escherichia coli J53. The resistance determinant was on conjugative plasmids that encoded a CMY-2, a plasmid-mediated AmpC beta-lactamase. To our knowledge, these are the first description of S. enterica serovar Rissen harboring CMY-2 in South Korea.
Song
Wonkeun
W
Department of Laboratory Medicine, Hallym University College of Medicine, Kangnam Sacred Heart Hospital, 948-1, Daelim-dong, Youngdeungpo-gu, Seoul 150-950, South Korea. swonkeun@hallym.or.kr
Kim
Jae-Seok
JS
Kim
Han-Sung
HS
Park
Min-Jeong
MJ
Lee
Kyu Man
KM
D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002440:Cefoxitin / Q000494:pharmacology; D018550:Cephalosporin Resistance / Q000235:genetics; D003227:Conjugation, Genetic; D003967:Diarrhea / Q000382:microbiology; D005260:Female; D006785:Hospitals, University; D006801:Humans; D007223:Infant; D007723:Korea; D008297:Male; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D012189:Retrospective Studies; D012480:Salmonella Infections / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002440:Cefoxitin; C103022:beta-lactamase CMY-2; C048795:AmpC beta-lactamases; D001618:beta-Lactamases