Asymmetric cooperativity in tandem hybridization of enantiomeric metal complex-tethered short fluorescent DNA probes.
The complex [Ru(phen)(2)(dppz)](2+)(phen = 1,10-phenanthroline, dppz = dipyrido[3,2-aratio2',3'-c]phenazine) was attached to the 5' end of a short oligonucleotide to form conjugates, the Delta-isomer of which showed a high cooperativity during the recognition of the repetitive sequence, while the Lambda-isomer did not.
Kitamura
Yusuke
Y
Department of Applied Chemistry and Biochemistry, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
Ihara
Toshihiro
T
Okada
Kenji
K
Tsujimura
Yusuke
Y
Shirasaka
Yoshinori
Y
Tazaki
Masato
M
Jyo
Akinori
A
D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D012091:Repetitive Sequences, Nucleic Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D015342:DNA Probes; D005456:Fluorescent Dyes; D008670:Metals; D009841:Oligonucleotides; D009942:Organometallic Compounds; C466600:Ru(phen)(2)dipyrido(3,2-a-2',3'-c)phenazine