Multiple-fluorophore-specie detection with a tapered Fabry-Perot fluorescence spectrometer.
A novel fluorescence spectrometer and method for the simultaneous detection of multiple-fluorophore species in a no-moving-parts, instantaneous manner is described. In the reported embodiment of the instrument, a tapered Fabry-Perot filter is used to spatially encode the fluorescence spectrum from a multiple-dye-containing test sample. Using a pseudoinverse reconstruction algorithm, we spectrally decode the particle concentration for each dye specie in the test sample. Experimental results are reported along with a theoretical treatment of the method.
Wahl
Jeremy A
JA
Department of Applied Physics, Cornell University, 115 Phillips Hall, Ithaca, New York 14853, USA. jaw45@cornell.edu
Van Delden
Jay S
JS
Tiwari
Sandip
S