Characterization and expression profile of AmphiCD63 encoding a novel member of TM4SF proteins from amphioxus Branchiostoma belcheri tsingtauense.
The study on CD antigen genes remains lacking in the cephalochordate amphioxus to date. In this report, the cDNA encoding CD63 was identified for the first time from the gut cDNA library of amphioxus Branchiostoma belcheri tsingtauense. Primary structural examination showed that the protein encoded by the cDNA contained four potential transmembrane domains characteristic of transmembrane 4 superfamily (TM4SF) proteins and a conserved CCG motif in the putative major extracellular loop. BLAST search revealed that the cDNA is closely associated with other known CD63 antigen genes, and it was thus designated AmphiCD63. Phylogenetic analysis indicated that AmphiCD63 is extremely close to vertebrate CD63, CD151 and CD53, suggesting they may have been evolved from a common ancestral gene. RT-PCR analysis exhibited that AmphiCD63 mRNA was abundant in muscle, ovary, foregut including hepatic caecum and hindgut, while it was present at considerably lower levels in notochord and gill and absent in testis.
Liu
Zhenhui
Z
Department of Marine Biology, Ocean University of China, Qingdao.
Zhang
Shicui
S
Li
Hongyan
H
Luan
Jing
J
Wang
Yongjun
Y
Wang
Lei
L
Xiang
Jianhai
J
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000737:chemistry; D015703:Antigens, CD / Q000737:chemistry* / Q000235:genetics*; D000954:Antigens, Surface / Q000737:chemistry; D001483:Base Sequence; D043344:Chordata; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D010980:Platelet Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics*; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D060149:Tetraspanin 30; D014018:Tissue Distribution
D000941:Antigens; D015703:Antigens, CD; D000954:Antigens, Surface; C554586:CD63 protein, human; D018076:DNA, Complementary; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D060149:Tetraspanin 30; D012313:RNA