Molecular characterization of five medicinally important species of Typhonium (Araceae) through random amplified polymorphic DNA (RAPD).
The interrelationship of five medicinally important species of Typhonium (Araceae) including T. venosum, which was previously placed under the genus Sauromatum, was inferred by analysis of random amplified polymorphic DNA (RAPD). DNA from pooled leaf samples was isolated and RAPD analysis was performed using 20 decamer oligonucleotide primers. Out of a total of 245 bands amplified, 12 were found to be monomorphic while 233 bands were polymorphic including 86 species-specific bands. The genetic similarities were analyzed from the dendrogram constructed by the pooled RAPD data using a similarity index. The dendrogram showed two distinct clades, one containing T. roxburghii, T. trilobatum and T. venosum and the other containing the remainder two species, i.e. T. diversifolium and T. flagelliforme. Both the clusters shared a common node approx. at 23.7% level of similarity. The maximum similarity of 31.2% was observed between T. venosum and T. trilobatum. In view of its close genetic similarity with other members of Typhonium, transfer of Sauromatum venosum to the genus Typhonium and merger of the two genera was supported.
Acharya
Laxmikanta
L
DNA Fingerprinting Laboratory, Plant Biotechnology Division, Regional Plant Resource Centre, Nayapalli, Bhubaneswar 751 015, Orissa, India.
Mukherjee
Arup Kumar
AK
Panda
Pratap Chandra
PC
Das
Premananda
P
D029064:Araceae / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D018745:Genome, Plant; D010802:Phylogeny; D010946:Plants, Medicinal / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D017931:DNA Primers; D004269:DNA, Bacterial; D018744:DNA, Plant