title: Expression of the adrenomedullin gene in adipose tissue.
abstract: Adrenomedullin (AM) is a potent vasodilating peptide originally isolated from human pheochromocytoma cells. This report concerns the expression and secretion of AM from adipose tissue. Northern blot analysis demonstrated marked expression of AM mRNA in mouse adipose tissue. Expression levels in adipose tissues were 2.5-3.2 times higher than in the kidney. AM mRNA level in mature adipocytes was 7.3 times higher than in the stroma-vascular fraction of adipose tissue. In mature adipocyte culture, time-dependent increase of AM peptide concentration in the culture medium was detected. AM expression was also detected in human subcutaneous adipose tissue. Adipose AM expression significantly increased in obesity mouse model, high-fat diet fed mice and ob/ob mice. These results suggest that adipose tissue, especially mature adipocytes, is major source of AM in the body, and that adipocyte-derived AM plays a pathophysiological role in obesity.
authors.affiliation: Department of Medicine and Clinical Science Kyoto University Graduate School of Medicine 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Takuo
authors.initials: T
authors.lastname: Nambu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Arai
authors.forename: Yasato
authors.initials: Y
authors.lastname: Komatsu
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yasoda
authors.forename: Kenji
authors.initials: K
authors.lastname: Moriyama
authors.forename: Naotetsu
authors.initials: N
authors.lastname: Kanamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Itoh
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D053607:Adrenomedullin; D000818:Animals; D015992:Body Mass Index; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000378:metabolism*; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D010455:Peptides; D012333:RNA, Messenger; D053607:Adrenomedullin