Tenascin-C promotes cell survival by activation of Akt in human chondrosarcoma cell.
Tenascin-C (TnC) is an extracellular matrix protein that is highly expressed in tumor stroma. In this report, we examined the roles of TnC-mediated cell adhesion in the modulation of chondrosarcoma cell survival. We found that hTnC-mediated adhesion could confer a significant (P<0.05) survival advantage to human chondrosarcoma cell line, JJ012, following serum-deprivation compared with the same cells grown on poly-lysine. This pro-survival signal was due to the activation of the Akt upon adhesion to hTnC. Moreover, hTnC-induced Akt activation was blocked by LY294002 and the expression of dominant-negative Akt. Taken together, these studies support that the TnC-mediated adhesion can promote cell survival through Akt in human chondrosarcoma cells.
Jang
Jun-Hyeog
JH
Department of Biochemistry, Inha University College of Medicine, Jung-gu, Incheon 400-712, South Korea. juhjang@inha.ac.kr
Chung
Chong-Pyoung
CP
D001859:Bone Neoplasms / Q000473:pathology*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002470:Cell Survival; D002813:Chondrosarcoma / Q000473:pathology*; D004789:Enzyme Activation; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt; D015398:Signal Transduction; D019063:Tenascin / Q000502:physiology*
D011518:Proto-Oncogene Proteins; D019063:Tenascin; C494918:AKT1 protein, human; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt