Optimization of bacteriocin ST311LD production by Enterococcus faecium ST311LD, isolated from spoiled black olives.
Bacteriocin ST311LD is approximately 2.3 kDa in size. Low levels of bacteriocin activity were recorded in BHI and M17 broth (800 AU/ml) and in 10% (w/v) soy milk (3,200 AU/ml). No bacteriocin production was recorded in 10% (w/v) molasses, despite good growth. Optimal levels (12,800 AU/ml) were detected in MRS broth which had been supplemented with tryptone (20.0 g/l), saccharose (5.0 or 10.0 g/l) or vitamin C (1 ppm). Increased potassium levels did not result in higher levels of activity, and glycerol (1.0 g/l) inhibited the production of bacteriocin ST311LD.
Todorov
Svetoslav D
SD
Department of Microbiology, Stellenbosch University, 7600 Stellenbosch, South Africa.
Dicks
Leon M T
LM
D001430:Bacteriocins / Q000096:biosynthesis*; D016984:Enterococcus faecium / Q000378:metabolism*; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D031658:Olea / Q000382:microbiology*
D001430:Bacteriocins