Assessment of heparin binding to the AN69 ST hemodialysis membrane: I. Preclinical studies.
The AN69 ST membrane was designed to render the surface of the native polyacrylonitrile polymer less cationic. This was achieved by layering the membrane with the polycationic biopolymer polyethyleneimine. This new membrane is able to bind heparin to its surface, through electrical interactions, without altering the reactivity of the sulfonate groups of the membrane, regularly distributed in the membrane bulk. The kinetics of unfractionated or low-molecular-weight heparins were studied in vitro and in vivo in sheep. Encouraging results were obtained indicating that heparin-coated hemodialyzers are potent anticoagulants. Priming the AN69 ST membrane-equipped hemodialyzer with heparin, as in regular hemodialysis, could allow drastic reduction of heparin consumption in hemodialysis.
Chanard
Jacques
J
Service de Néphrologie, Centre Hospitalier et Universitaire and CNRS FRE 2534 Reims, France.
Lavaud
Sylvie
S
Paris
Bruno
B
Toure
Fatouma
F
Rieu
Philippe
P
Renaux
Jean-Louis
JL
Thomas
Michel
M
D000180:Acrylic Resins; D000327:Adsorption; D000706:Anaphylatoxins / Q000378:metabolism; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D000990:Antithrombin III / Q000378:metabolism; D001672:Biocompatible Materials; D003124:Colorimetry; D003167:Complement Activation; D003176:Complement C3 / Q000096:biosynthesis; D016207:Cytokines / Q000145:classification / Q000493:pharmacokinetics; D017985:Dalteparin / Q000494:pharmacology; D017984:Enoxaparin / Q000494:pharmacology; D005112:Extracorporeal Circulation; D015951:Factor Xa / Q000378:metabolism; D065427:Factor Xa Inhibitors; D006493:Heparin / Q000378:metabolism*; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D007700:Kinetics; D008567:Membranes, Artificial; D017762:Nadroparin / Q000494:pharmacology; D010314:Partial Thromboplastin Time; D011094:Polyethyleneimine; D011485:Protein Binding; D006435:Renal Dialysis / Q000295:instrumentation* / Q000379:methods; D012756:Sheep
D000180:Acrylic Resins; D000706:Anaphylatoxins; D000925:Anticoagulants; D001672:Biocompatible Materials; D003176:Complement C3; D016207:Cytokines; D017984:Enoxaparin; D065427:Factor Xa Inhibitors; D006495:Heparin, Low-Molecular-Weight; D008567:Membranes, Artificial; D017762:Nadroparin; C010504:polyacrylonitrile; D000990:Antithrombin III; D011094:Polyethyleneimine; D006493:Heparin; D015951:Factor Xa; D017985:Dalteparin