Comparison of recombinant adenovirus and synthetic peptide for DC-based melanoma vaccination.
Optimal strategies for antigen-specific melanoma vaccination are currently being defined in experimental mouse models. Using a single H2-K(b)-binding peptide epitope derived from the melanosomal enzyme tyrosinase-related protein 2 (TRP2) in C57BL/6 mice, we show that adenovirus-transduced dendritic cells (DC) are clearly superior to peptide-pulsed DC for the induction of CD8+ T cells and antimelanoma immunity. Vaccine efficacy strictly depended on the presence of linked CD4+ T-cell help during the priming but not the effector phase of the immune response. These results provide important information for the translation of melanoma vaccine strategy in future clinical applications.
Steitz
J
J
Department of Dermatology, Laboratory of Experimental Dermatology, University of Bonn, Bonn, Germany.
Tormo
D
D
Schweichel
D
D
Tüting
T
T
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D015704:CD4 Antigens / Q000235:genetics / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D004274:DNA, Recombinant / Q000494:pharmacology; D003713:Dendritic Cells / Q000276:immunology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D008175:Lung Neoplasms / Q000276:immunology / Q000556:secondary / Q000628:therapy; D008546:Melanoma, Experimental / Q000276:immunology / Q000473:pathology / Q000517:prevention & control* / Q000628:therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014161:Transduction, Genetic; D014611:Vaccination
D015704:CD4 Antigens; D019496:Cancer Vaccines; D004274:DNA, Recombinant; D010446:Peptide Fragments; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase