title: Pumping of mammalian cells with a nozzle-diffuser micropump.
abstract: We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications.
authors.affiliation: Institute of Microelectronics and Microsystems, Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland. christophe.yamahata@a3.epfl.ch
authors.forename: Christophe
authors.initials: C
authors.lastname: Yamahata
authors.forename: Caroline
authors.initials: C
authors.lastname: Vandevyver
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lacharme
authors.forename: Paulina
authors.initials: P
authors.lastname: Izewska
authors.forename: Horst
authors.initials: H
authors.lastname: Vogel
authors.forename: Ruth
authors.initials: R
authors.lastname: Freitag
authors.forename: Martin A M
authors.initials: MA
authors.lastname: Gijs
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003585:Cell Biology; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D046508:Culture Techniques; D004058:Diffusion; D004867:Equipment Design; D006801:Humans; D006825:Hybridomas / Q000166:cytology; D019169:Jurkat Cells; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D013778:Tetrazolium Salts / Q000494:pharmacology; D013997:Time Factors; D014343:Trypan Blue / Q000494:pharmacology
chemical_list: C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; D013778:Tetrazolium Salts; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D014343:Trypan Blue; D002118:Calcium