Herminiimonas fonticola gen. nov., sp. nov., a Betaproteobacterium isolated from a source of bottled mineral water.
Several yellowish-pigmented bacteria with an optimum growth temperature of about 30 degrees C, were recovered from the source (borehole) of bottled mineral water in the Serra da Estrela in Eastern Portugal. Phylogenetic analyses of the 16S rRNA gene sequence of strains S-94T , S-97, S-99 and S-92 indicated that these organisms represent a new species of the Betaproteobacteria that is not closely related to any other known species. The major fatty acids of the strains are 16:1 omega7c and 16:0. Ubiquinone 8 is the major respiratory quinone. The new isolates are strictly organotrophic and aerobic. The new strains only assimilated organic acids, glycine and alanine. Casamino acids and a mixture of all natural amino acids are not used as sole carbon and nitrogen sources; these are used as nitrogen source in the presence of organic acids. On the basis of the phylogenetic analyses, physiological and biochemical characteristics, we are of the opinion that strains S-94T, S-97, S-99 and S-92 represent a new species of a novel genus for which we propose the name Herminiimonas fonticola gen. nov., sp. nov.
Fernandes
Chantal
C
Departamento de Bioquímica, Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3001-401 Coimbra, Portugal.
Rainey
Fred A
FA
Nobre
M Fernanda
MF
Pinhal
Isabel
I
Folhas
Fátima
F
da Costa
Milton S
MS
D001483:Base Sequence; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008900:Mineral Waters / Q000382:microbiology*; D008969:Molecular Sequence Data; D042802:Oxalobacteraceae / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D011174:Portugal; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D005227:Fatty Acids; D008900:Mineral Waters; D012336:RNA, Ribosomal, 16S