Synthesis and studies on antioxidants: ethoxyquin (EQ) and its derivatives.
In our study ethoxyquin (EQ) and its two complexes with flavonoids were obtained from ethoxyquin (1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline, EQ) and quercetin (EQ-Q, 1:1) or rutin (EQ-R, 1:1). Cytotoxicity of the tested compounds was studied using the trypan blue exclusion method and the properties of the studied compounds were also analyzed with the TUNEL method evaluating their ability to induce apoptosis. It was shown that EQ induced apoptosis in cultured human lymphocytes, especially at 0.25 and 0.5 mM concentrations. The same effects were also observed after the incubation of lymphocytes with EQ-Q and EQ-R, but the numbers of apoptotic cells observed were lower than for EQ.
Błaszczyk
Alina
A
Department of Cytogenetics and Plant Molecular Biology, University of Lódź , 12/16 Banacha Str., 90-237 Lódź.
Skolimowski
Janusz
J
D000975:Antioxidants / Q000138:chemical synthesis* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005015:Ethoxyquin / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D005520:Food Preservatives / Q000138:chemical synthesis; D006801:Humans; D020287:In Situ Nick-End Labeling; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D011310:Preservatives, Pharmaceutical / Q000138:chemical synthesis; D013997:Time Factors
D000975:Antioxidants; D005520:Food Preservatives; D011310:Preservatives, Pharmaceutical; D005015:Ethoxyquin