Genome-wide analysis of retroviral DNA integration.
Retroviral vectors are often used to introduce therapeutic sequences into patients' cells. In recent years, gene therapy with retroviral vectors has had impressive therapeutic successes, but has also resulted in three cases of leukaemia caused by insertional mutagenesis, which has focused attention on the molecular determinants of retroviral-integration target-site selection. Here, we review retroviral DNA integration, with emphasis on recent genome-wide studies of targeting and on the status of efforts to modulate target-site selection.
Bushman
Frederic
F
University of Pennsylvania School of Medicine, Department of Microbiology, 3610 Hamilton Walk, Philadelphia, Pennsylvania 19104-6076, USA. bushman@mail.med.upenn.edu
Lewinski
Mary
M
Ciuffi
Angela
A
Barr
Stephen
S
Leipzig
Jeremy
J
Hannenhalli
Sridhar
S
Hoffmann
Christian
C
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics; D011533:Proviruses / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D013045:Species Specificity; D016662:Virus Integration
D018076:DNA, Complementary