Retroviral targeting of proliferating endothelial cells.
Tumor growth requires the formation of new blood vessels by endothelial cells. Thus, surface molecules -- such as angiogenin receptors -- that are selectively expressed on growing endothelium represent an attractive target for directed delivery of compounds to tumor tissue. We attempted to obtain genetically engineered retroviral vectors targeted to the endothelium by inserting the human angiogenin sequence into Moloney murine leukemia virus envelope glycoprotein. Abundant expression of the chimeric protein could be verified. However, while being selective for proliferating human endothelial cells, the recombinant retroviral particles displayed low transduction efficiencies and thus have to be further improved.
Gornikiewicz
Alexander
A
Department of Surgery, Vienna General Hospital, Medical University of Vienna, Vienna, Austria.
Zommer
Anna
A
Jakesz
Raimund
R
Gnant
Michael
M
Brostjan
Christine
C
D016475:3T3 Cells; D043925:Angiogenesis Inducing Agents / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D042783:Endothelial Cells / Q000378:metabolism / Q000821:virology*; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D011991:Receptors, Virus / Q000378:metabolism; D012190:Retroviridae / Q000502:physiology*; D012191:Retroviridae Proteins / Q000235:genetics*; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism
D043925:Angiogenesis Inducing Agents; D006023:Glycoproteins; D011991:Receptors, Virus; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins