S434F in NrdE generates the thermosensitive phenotype of corynebacterium ammoniagenes CH31 and enhances thermolability by increasing the surface hydrophobicity of the NrdE(Ts) protein.
The thermosensitive phenotype of strain CH31, a derivative of Corynebacterium ammoniagenes ATCC 6872, was allocated by cloning, sequencing, and genetic complementation to a single C-->T exchange in the nrdE (nucleotide reduction) gene at nucleotide 1301. Protein modeling indicates the impaired surface hydrophobicity of NrdE(Ts) due to the S434F transition.
Elhariry
Hesham M
HM
Institut für Mikrobiologie der Universität, Schneiderberg 50, D-30167 Hannover, Germany. auling@ifmb.uni-hannover.de
Meens
Jochen
J
Stehr
Matthias
M
Auling
Georg
G
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003352:Corynebacterium / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D004795:Enzyme Stability; D006358:Hot Temperature; D057927:Hydrophobic and Hydrophilic Interactions; D008769:Methylnitronitrosoguanidine; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010641:Phenotype; D017354:Point Mutation; D012264:Ribonucleotide Reductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D008769:Methylnitronitrosoguanidine; D012264:Ribonucleotide Reductases