title: Paenibacillus motobuensis sp. nov., isolated from a composting machine utilizing soil from Motobu-town, Okinawa, Japan.
abstract: A novel bacterial strain, MC10(T), was isolated from a compost sample produced in a composting machine utilizing soil from Motobu-town, Okinawa, Japan. The isolate was Gram-negative, but produced endospores. These conflicting characters prompted a taxonomic study of the isolate. The isolate was examined using a combination of phenotypic characterization, cellular fatty acid analysis, DNA base composition determination and 16S rRNA gene sequence analysis. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, placed strain MC10(T) within the genus Paenibacillus. As in other Paenibacillus species, the isolate contained anteiso-C(15:0) as the major fatty acid and the DNA G+C content was 47.0 mol%. However, 16S rRNA gene sequence similarity values of less than 95.6% were found between this isolate and all members of the genus Paenibacillus. Based upon these results, strain MC10(T) (=GTC 1835(T)=JCM 12774(T)=CCUG 50090(T)) should be designated as the type strain of a novel species within the genus Paenibacillus, Paenibacillus motobuensis sp. nov.
authors.affiliation: Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-city, Fukuoka, 812-8582, Japan.
authors.forename: Ken-Ichiro
authors.initials: KI
authors.lastname: Iida
authors.affiliation: Motobu Noge Hospital, 880-1 Ohama, Motobu-cho, Kunigami-gun, Okinawa, 905-0212, Japan.
authors.forename: Yasuichi
authors.initials: Y
authors.lastname: Ueda
authors.affiliation: Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu-city, Gifu, 501-1194, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kawamura
authors.affiliation: Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu-city, Gifu, 501-1194, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ezaki
authors.affiliation: Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-city, Fukuoka, 812-8582, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Takade
authors.affiliation: Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-city, Fukuoka, 812-8582, Japan.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Yoshida
authors.affiliation: Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-city, Fukuoka, 812-8582, Japan.
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Amako
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D007564:Japan; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal / Q000295:instrumentation* / Q000379:methods; D012988:Soil Microbiology; D013171:Spores, Bacterial
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S