[Does smoking influence the course of Crohn's disease in patients with the presence of gene NOD2/CARD15 mutation?].
Inflammatory bowel diseases can be divided into two diseases: ulcerative colitis and Crohn's disease. It is well known that there is the influence of smoking on these course of diseases and the number of flares. This influence is different in both diseases and is definitely negative among patients with Crohn's disease. The aim of the study was to establish if there is a difference of smoking influence on the course of disease depending if the patient is the carrier of NOD2/CARD15 mutation or not. 150 patients with CD was examined by careful interview about smoking habits, physically and there was molecular analyze performed of monocytes' DNA. The most often variant of NOD2/CARD15 mutation in Polish population was 802 C>T which causes the conversion of proline into serine in 268 position of protein product. The second most often observed variant was found during the analyze of exon 11 in the temperature of 20 degrees C. There was a frameshift mutation 3020insC present in 14.9% patients with CD which causes the C insertion in 3020 position of protein product. All patients with the frameshift mutation were also carriers of 802C>T mutation. The analysed features were the course of disease, the smoking habit and the difference in group of NOD2/CARD15 carries and patients without the mutation. We took into consideration the fact if the affected person was smoker, ex-smoker or non-smoker. Patients with 802C>T mutation ex-smokers and smokers were older on the onset of the disease (average: 35.8 years), whereas the non-smokers (average: 26.07 years). And what is more interesting, the non-smoking patients were statistically less frequent operated (the partial resection of terminal ileum). Only 31.82% of non-smoking patient with 802C>T mutation were operated.
Dobrowolska-Zachwieja
Agnieszka
A
Klinika Gastroenterologii, Zywienia Człowieka i Chorób Wewnetrznych Akademii Medycznej w Poznaniu.
Kaczmarek
Marta
M
Hoppe-Gołebiewska
Justyna
J
Kalak
Robert
R
Słomski
Ryszard
R
Linke
Krzysztof
K
D003424:Crohn Disease / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009154:Mutation; D053473:Nod2 Signaling Adaptor Protein; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012907:Smoking / Q000009:adverse effects*
D047908:Intracellular Signaling Peptides and Proteins; C429018:NOD2 protein, human; D053473:Nod2 Signaling Adaptor Protein