title: Increase in liver antioxidant enzyme activities in non-alcoholic fatty liver disease.
abstract: OBJECTIVE
  Steatosis may increase oxidative stress, which is counteracted by cellular enzymatic (cytosolic and mitochondrial superoxide dismutases (Cu/Zn-SOD and Mn-SOD), glutathione peroxidase (GPx), catalase) and non-enzymatic antioxidant systems. We aimed to determine, in patients with non-alcoholic fatty liver disease (NAFLD), the level of antioxidant defenses (1) in liver biopsies, to demonstrate the existence of oxidative stress; (2) in erythrocytes and plasma, to determine whether their antioxidant defenses reflect liver oxidative stress.
  
  
  METHODS
  Erythrocyte and plasma antioxidant defenses were prospectively studied in two groups of 16 patients: patients with NAFLD and controls. Liver biopsies were performed in eight NAFLD patients; liver antioxidant enzyme activities were measured and compared with those in 12 control livers used for transplantation.
  
  
  RESULTS
  Cu/Zn-SOD, GPx and catalase activities were significantly higher in NAFLD livers than in controls whereas no significant differences were observed in Mn-SOD activity, and thiobarbituric acid-reactive substance (TBARS) concentration. No differences were observed in erythrocyte antioxidant enzyme activities (GPx, catalase, Cu/Zn-SOD), erythrocyte TBARS concentration, and plasma alpha-tocopherol concentration.
  
  
  CONCLUSIONS
  Liver antioxidant enzyme activities were high in patients with NAFLD, reflecting an oxidative stress possibly involved in inflammation and fibrogenesis. However, erythrocyte and plasma antioxidant defenses did not reflect intrahepatic peroxidation.
authors.affiliation: Service d'hépato-gastroentérologie, Hôpital Antoine Béclère, AP-HP, 157 rue de la Porte de Trivaux, 92141 Clamart Cedex, France. gabriel.perlemuter@abc.aphp.fr
authors.forename: Gabriel
authors.initials: G
authors.lastname: Perlemuter
authors.forename: Anne
authors.initials: A
authors.lastname: Davit-Spraul
authors.forename: Claudine
authors.initials: C
authors.lastname: Cosson
authors.forename: Marc
authors.initials: M
authors.lastname: Conti
authors.forename: Amélie
authors.initials: A
authors.lastname: Bigorgne
authors.forename: Valérie
authors.initials: V
authors.lastname: Paradis
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Corre
authors.forename: Lydie
authors.initials: L
authors.lastname: Prat
authors.forename: Viceth
authors.initials: V
authors.lastname: Kuoch
authors.forename: Arnaud
authors.initials: A
authors.lastname: Basdevant
authors.forename: Gilles
authors.initials: G
authors.lastname: Pelletier
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Oppert
authors.forename: Catherine
authors.initials: C
authors.lastname: Buffet
mesh_terms: D000328:Adult; D000368:Aged; D002374:Catalase / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology; D005234:Fatty Liver / Q000201:enzymology*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase