title: Detection of C-reactive protein utilizing magnetic permeability detection based immunoassays.
abstract: A new sensing technology platform integrating magnetic permeability detection and a two-site heterogeneous immunoassay in a one-step analysis is described. As a platform model, measurements of C-reactive protein (CRP), a cardiac and inflammation marker, were performed in a rapid (11.5 min) high-sensitivity (hs) procedure with a low detection limit (0.2 mg/L) and accuracy (CV = 11%). The two-site heterogeneous immunoassay was performed in 1.2-mL disposable reagents vials containing solid phase (polyclonal anti-CRP conjugated silica microparticles), labeling agent (monoclonal anti-CRP conjugated superparamagnetic nanoparticles), and reaction buffer. Whole blood (20 muL) was assayed by introducing the sample into a reagent vial using a glass capillary and mixing its contents by hand for 30 s. After a 11-min sedimentation step, the vial was placed into the coil of the magnetic permeability detector, which measured the enrichment of superparamagnetic nanoparticles in the solid-phase sediment. Magnetic permeability detection and quantification is based on the principle that when paramagnetic materials are placed inside a coil, the inductance of the coil is influenced. Screening of CRP on whole blood patient samples showed good correlation with central hospital measurements for hsCRP (y = 1.018x - 0.021, R(2) = 0.980, n = 103) and normal range CRP (y = 1.02x + 2.53, R(2)= 0.991, n = 33) analyses. The mean differences of the two methods according to the Bland and Altman plots were -0.03 +/- 1.12 mg/L for hsCRP analysis and -3.4 +/- 8.64 mg/L for normal range CRP analysis.
authors.affiliation: Department of Pure and Applied Biochemistry, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Kirstin
authors.initials: K
authors.lastname: Kriz
authors.forename: Filiz
authors.initials: F
authors.lastname: Ibraimi
authors.forename: Min
authors.initials: M
authors.lastname: Lu
authors.forename: Lars-Olof
authors.initials: LO
authors.lastname: Hansson
authors.forename: Dario
authors.initials: D
authors.lastname: Kriz
mesh_terms: D002097:C-Reactive Protein / Q000032:analysis* / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D007118:Immunoassay; D007700:Kinetics; D008280:Magnetics; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D010539:Permeability
chemical_list: D002097:C-Reactive Protein