title: Identification of new antigens in visceral leishmaniasis by expression cloning and immunoblotting with sera of kala-azar patients from Bihar, India.
abstract: Sera of kala-azar patients from Bihar, India, were used to identify Leishmania donovani antigens encoded by a phage expression library. Ten antigens were identified, five of which have not been described as leishmania antigens before. The antigens specifically react with sera of leishmania-infected patients but not of toxoplasma- or plasmodium-infected patients.
authors.affiliation: Clinical Research Group, Department of Dermatology, Charité University Medicine Berlin, Humboldt University, Germany.
authors.forename: Stephan M
authors.initials: SM
authors.lastname: Theinert
authors.forename: Rajatava
authors.initials: R
authors.lastname: Basu
authors.forename: Michael
authors.initials: M
authors.lastname: Forgber
authors.forename: Syamal
authors.initials: S
authors.lastname: Roy
authors.forename: Shyam
authors.initials: S
authors.lastname: Sundar
authors.forename: Peter
authors.initials: P
authors.lastname: Walden
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology*; D000953:Antigens, Protozoan / Q000032:analysis* / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D006801:Humans; D015151:Immunoblotting; D007194:India; D007893:Leishmania donovani / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology*; D008969:Molecular Sequence Data
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan