Peyer patches are not required for acute graft-versus-host disease after myeloablative conditioning and murine allogeneic bone marrow transplantation.
Graft-versus-host disease (GVHD) is a multistep disease process following allogeneic bone marrow transplantation (BMT). It has been postulated that the induction of acute GVHD requires the presence of Peyer patches (PPs). A new tumor necrosis factor (TNF)-deficient strain has been developed that totally lacks PPs and displays the defects characteristic of TNF ablation but not lymphotoxin-associated defects characterized by lack of both PPs and lymph nodes. To determine the necessity of PPs in acute lethal GVHD induction, we transplanted full major histocompatibility complex (MHC)-mismatched grafts into myeloablated TNF knockout recipients. No differences in the survival or GVHD-associated histopathologic lesions were observed between the recipients. We conclude that neither PPs nor host TNF-alpha is required for the development of acute lethal GVHD in mice that undergo myeloablative conditioning and allogeneic BMT.
Welniak
Lisbeth A
LA
Department of Microbiology and Immunology, University of Nevada School of Medicine, Mail Stop 199, Reno, NV 89557, USA. lwelniak@unr.edu
Kuprash
Dmitry V
DV
Tumanov
Alexei V
AV
Panoskaltsis-Mortari
Angela
A
Blazar
Bruce R
BR
Sun
Kai
K
Nedospasov
Sergei A
SA
Murphy
William J
WJ
D000208:Acute Disease; D000818:Animals; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D006086:Graft vs Host Disease / Q000209:etiology* / Q000473:pathology; D007410:Intestinal Diseases / Q000209:etiology; D051379:Mice; D018345:Mice, Knockout; D010581:Peyer's Patches / Q000502:physiology*; D019172:Transplantation Conditioning / Q000379:methods; D014184:Transplantation, Homologous; D014409:Tumor Necrosis Factor-alpha / Q000172:deficiency
D014409:Tumor Necrosis Factor-alpha