An assessment of the effects of shell cross-linked nanoparticle size, core composition, and surface PEGylation on in vivo biodistribution.
Amphiphilic core-shell nanoparticles have drawn considerable interest in biomedical applications. The precise control over their physicochemical parameters and the ability to attach various ligands within specific domains suggest shell cross-linked (SCK) nanoparticles may be used as multi-/polyvalent scaffolds for drug delivery. In this study, the biodistribution of four SCKs, differing in size, core composition, and surface PEGylation, was evaluated. To facilitate in-vivo tracking of the SCKs, the positron-emitting radionuclide copper-64 was used. By using biodistribution and microPET imaging approaches, we found that small diameter (18 nm) SCKs possessing a polystyrene core showed the most favorable biological behavior in terms of prolonged blood retention and low liver accumulation. The data demonstrated that both core composition, which influenced the SCK flexibility and shape adaptability, and hydrodynamic diameter of the nanoparticle play important roles in the respective biodistributions. Surface modification with poly(ethylene glycol) (PEG) had no noticeable effects on SCK behavior.
Sun
Xiankai
X
Division of Radiological Sciences, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Rossin
Raffaella
R
Turner
Jeffrey L
JL
Becker
Matthew L
ML
Joralemon
Maisie J
MJ
Welch
Michael J
MJ
Wooley
Karen L
KL
D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002614:Chelating Agents / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D008024:Ligands; D051379:Mice; D008823:Micelles; D008956:Models, Chemical; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D049268:Positron-Emission Tomography; D017434:Protein Structure, Tertiary; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D001672:Biocompatible Materials; D002614:Chelating Agents; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D008024:Ligands; D008823:Micelles; D011108:Polymers; D011137:Polystyrenes; D011092:Polyethylene Glycols; D003300:Copper