Evaluation of two TaqMan PCR assays for the detection of HIV-1 proviral DNA in blood samples.
This study compared two published TaqMan PCR assays targeting different regions of the HIV-1 genome for detection of HIV-1 proviral DNA. The gag specific PCR demonstrated a lower sensitivity than the assay targeting the LTR region. The LTR assay is a highly reproducible and specific technique for HIV-1 proviral DNA detection.
Moroney
Shannon M
SM
Esoteric Testing, Department of Pathology, Tampa General Hospital, P.O. Box 1289, Tampa, FL 33601, USA. smoroney@tgh.org
Heller
Loree C
LC
Widen
Ray H
RH
D004279:DNA, Viral / Q000097:blood*; D015750:Genes, gag; D015658:HIV Infections / Q000175:diagnosis / Q000821:virology; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019914:Taq Polymerase
D004279:DNA, Viral; D019914:Taq Polymerase