Topography of c-fos immunoreactivity in an ovine head impact model.
Cortical neuronal and glial c-fos immunoreactivity has been demonstrated in experimental and human brain injury. c-fos is one of the immediate early genes important in signal transduction linking environmental stimuli to the cellular genome. c-fos immunoreactivity was semi-quantitated in a head impact sheep model using a grid system applied to standard coronal brain sections obtained from 12 impacted and 4 control sheep. Substantial glial and neuronal c-fos immunoreactivity was present in the pericontusional (penumbra) region, but was absent or minimal in the core of the contusion. Apart from these focal changes, c-fos immunoreactivity was diffusely distributed, with greater involvement in the cerebrum on the side of impact. In the cerebellum, Bergmann glia showed prominent c-fos immunoreactivity, while Purkinje cells were consistently immunonegative. c-fos immunoreactivity varied in different regions of the brain (focal and diffuse patterns) in this ovine head impact model.
Finnie
J
J
Hanson Institute Centre for Neurological Diseases, Institute of Medical and Veterinary Science and Department of Pathology, University of Adelaide, Adelaide, Australia.
Manavis
J
J
Jones
N
N
Blumbergs
P
P
D000818:Animals; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology / Q000662:veterinary*; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D012756:Sheep; D013702:Temporal Lobe / Q000473:pathology; D013997:Time Factors
D016760:Proto-Oncogene Proteins c-fos