The amino-terminal residue of glycoprotein B is critical for neutralization of bovine herpesvirus 1.
In order to address the neutralization epitope on bovine herpesvirus 1 (BHV1) glycoprotein B (gB), a panel of monoclonal antibodies (MAbs), a series of truncation forms of the glycoprotein and an MAb-escape mutant were used in this study. Immunocytochemistry on the truncations using MAbs against the glycoprotein revealed that the neutralization epitopes recognized by the MAbs lay between residues 1 and 52 of mature gB. Comparison of the sequences among the mutant, parent, and revertant viruses demonstrated that the amino-terminal residue of mature gB of the escape mutant was changed from Arg to Gln. These findings indicate that the amino-terminal residue of gB is critical for neutralization of BHV1.
Okazaki
Katsunori
K
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. kokazaki@hoku-iryo-u.ac.jp
Fujii
Sanae
S
Takada
Ayato
A
Kida
Hiroshi
H
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009154:Mutation; D020125:Mutation, Missense; D009500:Neutralization Tests; D017384:Sequence Deletion; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D010948:Viral Plaque Assay; D014764:Viral Proteins
D000914:Antibodies, Viral; D004279:DNA, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins