title: cAMP regulates axon outgrowth and guidance during optic nerve regeneration in goldfish.
abstract: Increased cAMP improves neuronal survival and axon regeneration in mammals. Here, we assess cAMP levels and identify activated pathways in a spontaneously regenerating central nervous system. Following optic nerve crush in goldfish, almost all retinal ganglion cells (RGC) survive and regenerate retinotectal topography. Goldfish received injections of a cAMP analogue (CPT-cAMP), a protein kinase A (PKA) inhibitor (KT5720), both compounds combined, or PBS (control). RGC survival in experimental groups was unaffected at any stage. The rate of axon regeneration was accelerated by the activator and decelerated both by the inhibitor and by combined injections, suggesting a PKA-dependent pathway. In addition, errors in regenerate retinotectal topography were observed when agents were applied in vivo and RGC response to the guidance cue ephrin-A5 in vitro was altered by the inhibitor. Our results highlight that therapeutic manipulation of cAMP levels to enhance axonal regeneration in mammals must ensure that topography, and consequently function, is not disrupted.
authors.affiliation: School of Animal Biology, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Western Australia. jrodger@cyllene.uwa.edu.au
authors.forename: J
authors.initials: J
authors.lastname: Rodger
authors.forename: H
authors.initials: H
authors.lastname: Goto
authors.forename: Q
authors.initials: Q
authors.lastname: Cui
authors.forename: P B
authors.initials: PB
authors.lastname: Chen
authors.forename: A R
authors.initials: AR
authors.lastname: Harvey
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003463:Cues; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D036386:Ephrin-A5 / Q000378:metabolism; D006054:Goldfish / Q000033:anatomy & histology / Q000378:metabolism*; D020439:Growth Cones / Q000378:metabolism* / Q000648:ultrastructure; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000502:physiology*; D009900:Optic Nerve / Q000166:cytology / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013477:Superior Colliculi / Q000166:cytology / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D036386:Ephrin-A5; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases