title: Evidence for a relationship between activity and the tetraprotomeric assembly of solubilized pig gastric H/K-ATPase.
abstract: Activity-oligomeric assembly relationships using octaethylene glycol dodecyl ether (C12E8) solubilized pig gastric H/K-ATPase (unmodified H/K-ATPase) or H/K-ATPase modified with Fluorescein 5'-isothiocyanate (FITC-H/K-ATPase) were examined. The amount of oligomeric species in FITC-H/K-ATPase, which retained little H/K-ATPase activity was estimated by a single-molecule detection technique using total internal reflection fluorescence microscopy. Solubilization of the FITC-H/K-ATPase reduced the potassium-dependent p-nitrophenyl phosphatase (K-pNPPase) activity to around 5% of the level of the membrane-bound enzyme with the formation of 50% protomer and 40% diprotomer. The solubilization of unmodified H/K-ATPase also reduced both the K-pNPPase and H/K-ATPase activities to around 5%. However, solubilization with increasing concentrations of potassium acetate induced significant and similar increases in K-pNPPase activity (K0.5 = 35 mM) with an increase in the amount of the tetraprotomer of FITC-H/K-ATPase, and the K-pNPPase (K0.5 = 28 mM) and H/K-ATPase (K0.5 = 40 mM) activities of the unmodified H/K-ATPase. The correlation coefficient between the proportion of tetraprotomer and the proportion of the K-pNPPase activity for the same FITC-H/K-ATPase preparation was estimated to be 0.93. Similar coefficients were also obtained between the proportion of tetraprotomer in the FITC-H/K-ATPase and the proportion of K-pNPPase and H/K-ATPase activities in the unmodified H/K-ATPase, with value of 0.85 and 0.86, respectively. Such positive correlations were not obtained between these activities and other oligomeric species. These data, the first direct comparison of oligomeric assembly and enzyme activity both stabilized by K+ in C12E8-solubilized gastric H/K-ATPase, provide strong evidence that the catalytic unit of C12E8-solubilized gastric H/K-ATPase is a tetraprotomer.
authors.affiliation: Biological Chemistry, Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Abe
authors.forename: Shunji
authors.initials: S
authors.lastname: Kaya
authors.forename: Kazuya
authors.initials: K
authors.lastname: Taniguchi
authors.forename: Yutaro
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Imagawa
authors.forename: Mahito
authors.initials: M
authors.lastname: Kikumoto
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Oiwa
authors.forename: Kazuyasu
authors.initials: K
authors.lastname: Sakaguchi
mesh_terms: D009597:4-Nitrophenylphosphatase / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D004987:Ethers / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D005753:Gastric Mucosa / Q000201:enzymology*; D017506:H(+)-K(+)-Exchanging ATPase / Q000737:chemistry* / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D019347:Potassium Acetate; D020836:Protein Structure, Quaternary; D013552:Swine
chemical_list: D004987:Ethers; D005456:Fluorescent Dyes; D009597:4-Nitrophenylphosphatase; D017506:H(+)-K(+)-Exchanging ATPase; D016650:Fluorescein-5-isothiocyanate; D019347:Potassium Acetate