title: Screening of plants acting against Heterometrus laoticus scorpion venom activity on fibroblast cell lysis.
abstract: The aqueous extracts of 64 plant species, listed as animal- or insect-bite antidotes in old Thai drug recipes were screened for their activity against fibroblast cell lysis after Heterometrus laoticus scorpion venom treatment. The venom was preincubated with plant extract for 30 min and furthered treated to confluent fibroblast cells for 30 min. More than 40% efficiency (test/control) was obtained from cell treatment with venom preincubated with extracts of Andrographis paniculata Nees (Acanthaceae), Barringtonia acutangula (L.) Gaertn. (Lecythidaceae), Calamus sp. (Palmae), Clinacanthus nutans Lindau (Acanthaceae), Euphorbia neriifolia L. (Euphorbiaceae), Ipomoea aquatica Forssk (Convolvulaceae), Mesua ferrea L. (Guttiferae), Passiflora laurifolia L. (Passifloraceae), Plectranthus amboinicus (Lour.) Spreng. (Labiatae), Ricinus communis L. (Euphorbiaceae), Rumex sp. (Polygonaceae) and Sapindus rarak DC. (Sapindaceae), indicating that they had a tendency to be scorpion venom antidotes. However, only Andrographis paniculata and Barringtonia acutangula extracts provided around 50% viable cells from extract treatments without venom preincubation. These two plant extracts are expected to be scorpion venom antidotes with low cytotoxicity.
authors.affiliation: Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Nunthawun
authors.initials: N
authors.lastname: Uawonggul
authors.forename: Arunrat
authors.initials: A
authors.lastname: Chaveerach
authors.forename: Sompong
authors.initials: S
authors.lastname: Thammasirirak
authors.forename: Tarinee
authors.initials: T
authors.lastname: Arkaravichien
authors.forename: Chattong
authors.initials: C
authors.lastname: Chuachan
authors.forename: Sakda
authors.initials: S
authors.lastname: Daduang
mesh_terms: D000818:Animals; D000997:Antivenins / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D004353:Drug Evaluation, Preclinical; D005347:Fibroblasts / Q000187:drug effects*; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000627:therapeutic use*; D012604:Scorpion Venoms / Q000037:antagonists & inhibitors*; D012605:Scorpions; D013785:Thailand
chemical_list: D000997:Antivenins; D010936:Plant Extracts; D012604:Scorpion Venoms