Attenuated expression of DFFB is a hallmark of oligodendrogliomas with 1p-allelic loss.
Allelic loss of chromosome 1p is frequently observed in oligodendroglioma. We screened 177 oligodendroglial tumors for 1p deletions and found 6 tumors with localized 1p36 deletions. Several apoptosis regulation genes have been mapped to this region, including Tumor Protein 73 (p73), DNA Fragmentation Factor subunits alpha (DFFA) and beta (DFFB), and Tumor Necrosis Factor Receptor Superfamily Members 9 and 25 (TNFRSF9, TNFRSF25). We compared expression levels of these 5 genes in pairs of 1p-loss and 1p-intact tumors using quantitative reverse-transcriptase PCR (QRTPCR) to test if 1p deletions had an effect on expression. Only the DFFB gene demonstrated decreased expression in all tumor pairs tested. Mutational analysis did not reveal DFFB mutations in 12 tested samples. However, it is possible that DFFB haploinsufficiency from 1p allelic loss is a contributing factor in oligodendroglioma development.
McDonald
J Matthew
JM
Department of Pathology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, USA. jonmatth@mdanderson.org
Dunmire
Valerie
V
Taylor
Ellen
E
Sawaya
Raymond
R
Bruner
Janet
J
Fuller
Gregory N
GN
Aldape
Kenneth
K
Zhang
Wei
W
D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D003851:Deoxyribonucleases / Q000235:genetics* / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D009837:Oligodendroglioma / Q000235:genetics* / Q000378:metabolism*; D000075223:Poly-ADP-Ribose Binding Proteins; D012333:RNA, Messenger / Q000235:genetics
D000075223:Poly-ADP-Ribose Binding Proteins; D012333:RNA, Messenger; C113696:DFFB protein, human; D003851:Deoxyribonucleases