Sulfato/thiosulfato reducing bacteria characterization by FT-IR spectroscopy: a new approach to biocorrosion control.
Sulfato and Thiosulfato Reducing Bacteria (SRB, TRB) can induce corrosion process on steel immersed in seawater. This phenomenon, called biocorrosion, costs approximatively 5 billion euros in France each year. We provide the first evidence that Fourier Transformed InfraRed (FTIR) spectroscopy is a competitive technique to evaluate the sulfurogen flora involved in biocorrosion in comparison with time consuming classical identification methods or PCR analyses. A great discrimination was obtained between SRB, TRB and some contamination bacteria known to be present in seawater and seem to be able to reduce sulfate under particular conditions. Moreover, this preliminary study demonstrates that FTIR spectroscopic and genotypic results present a good correlation (these results are confirmed by other data obtained before or later, data not shown here). The advantages gained by FTIR spectroscopy are to give information on strain phenotype and bacterial metabolism which are of great importance in corrosion processes.
Rubio
Celine
C
Corrodys, Equipe de Recherche en Physico-Chimie et Biotechnologie, EA 1852, IUT Caen, Université Caen Basse Normandie, 14032 Caen Cedex, France.
Ott
Christelle
C
Amiel
Caroline
C
Dupont-Moral
Isabelle
I
Travert
Josette
J
Mariey
Laurence
L
D018441:Biofilms; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D016999:Sulfur-Reducing Bacteria / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D014871:Water Microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S