A theoretical analysis of codon adaptation index of the Boophilus microplus bm86 gene directed to the optimization of a DNA vaccine.
DNA vaccines utilize host cell molecules for gene transcription and translation to proteins, and the interspecific difference of codon usage is one of the major obstacles for effective induction of specific and strong immune response. In an attempt to improve codon usage effects of DNA vaccine on protein expression, a quantitative study was conducted to clarify the relationship of codon usage in the tick gene bm86 and its potential expression in bovine cells. The calculated relative synonymous codon usage (RSCU) and codon adaptation index (CAI) values of bm86 from Boophilus microplus and a set of 14 highly expressed genes from Bos taurus indicated that some codons utilized frequently in bm86 are rarely used in B. taurus genes and vice versa. The different translational efficiencies obtained suggested that after DNA vaccination using the wild bm86 gene, the protein Bm86 would be expressed in bovines, but it would not be the optimum sequence. However, using the codon-optimized bm86 gene to bovines, whose sequence was theoretically designed, would probably improve the level of the immune response generated against ticks.
Ruiz
Lina María
LM
Unidad de Biotecnología y Control Biológico, Corporación para Investigaciones Biológicas, Apartado Aéreo 7378, Carrera 72A #78B-141, Medellín, Colombia.
Armengol
Gemma
G
Habeych
Edwin
E
Orduz
Sergio
S
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology / Q000517:prevention & control*; D003062:Codon; D015870:Gene Expression; D008562:Membrane Glycoproteins / Q000235:genetics*; D008957:Models, Genetic; D018448:Models, Immunological; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics*; D013984:Tick Infestations / Q000517:prevention & control*; D013987:Ticks / Q000235:genetics; D014611:Vaccination; D014612:Vaccines / Q000235:genetics*; D019444:Vaccines, DNA / Q000235:genetics*
C085065:BM86 protein, Boophilus; D003062:Codon; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014612:Vaccines; D019444:Vaccines, DNA