Modifier loci condition autoimmunity provoked by Aire deficiency.
Loss of function mutations in the autoimmune regulator (Aire) gene in autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy patients and mutant mice lead to autoimmune manifestations that segregate as a monogenic trait, but with wide variation in the spectrum of organs targeted. To investigate the cause of this variability, the Aire knockout mutation was backcrossed to mice of diverse genetic backgrounds. The background loci strongly influenced the pattern of organs that were targeted (stomach, eye, pancreas, liver, ovary, thyroid, and salivary gland) and the severity of the targeting (particularly strong on the nonobese diabetic background, but very mild on the C57BL/6 background). Autoantibodies mimicked the disease pattern, with oligoclonal reactivity to a few antigens that varied between Aire-deficient strains. Congenic analysis and a whole genome scan showed that autoimmunity to each organ had a distinctive pattern of genetic control and identified several regions that controlled the pattern of targeting, including the major histocompatibility complex and regions of Chr1 and Chr3 previously identified in controlling type 1 diabetes.
Jiang
Wenyu
W
Section on Immunology and Immunogenetics, Joslin Diabetes Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02215, USA.
Anderson
Mark S
MS
Bronson
Roderick
R
Mathis
Diane
D
Benoist
Christophe
C
D000818:Animals; D001327:Autoimmune Diseases / Q000235:genetics*; D005260:Female; D005756:Gastritis / Q000235:genetics / Q000276:immunology; D005819:Genetic Markers / Q000276:immunology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D009928:Organ Specificity; D010179:Pancreas / Q000378:metabolism / Q000473:pathology; D010195:Pancreatitis / Q000235:genetics / Q000276:immunology; D010641:Phenotype; D012720:Severity of Illness Index; D014157:Transcription Factors / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D000096927:AIRE Protein
D005819:Genetic Markers; D014157:Transcription Factors; D000096927:AIRE Protein