Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China.
Two moderately halophilic spore-forming bacteria were isolated from salt lakes in the Xinjiang region of China. The two strains, designated AD-6(T) and D-8(T), were aerobic, Gram-positive, rod-shaped and motile by means of peritrichous flagella. Strains AD-6(T) and D-8(T) grew in the presence of 0.5-20% and 0.5-25% (w/v) NaCl in complex medium, respectively. Their cell-wall peptidoglycan was of the L-Orn-D-Asp type. The major menaquinone found in both strains was menaquinone-7 (MK-7). The fatty acid profile contained a large amount of branched fatty acids; the main fatty acids were anteiso-C(15:0), anteiso-C(17:0), iso-C(15:0) and iso-C(16:0). The DNA G+C content of strains D-8(T) and AD-6(T) was 41.4 and 42.2 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains D-8(T) and AD-6(T) were located in the genus Halobacillus. Levels of 16S rRNA gene sequence similarity between the isolated strains and the type strains of Halobacillus species were in the range 96.2-99.5%. DNA-DNA relatedness values of 17.0-52.2% were found between the two strains and other Halobacillus species. The DNA-DNA relatedness value between D-8(T) and AD-6(T) was 50.6%. On the basis of phenotypic and chemotaxonomic properties, phylogenetic analysis and genomic distinctiveness, strains D-8(T) and AD-6(T) should be placed in the genus Halobacillus as two novel species, for which the names Halobacillus dabanensis sp. nov. (type strain=JCM 12772(T)=CGMCC 1.3704(T)) and Halobacillus aidingensis sp. nov. (type strain=JCM 12771(T)=CGMCC 1.3703(T)) are proposed, respectively.
Liu
W Y
WY
Division of Mineral Resources, Metallurgy and Materials, General Research Institute for Nonferrous Metals, Beijing, 100088, P. R. China.|Department of Microbiology, College of Biological Sciences, China Agricultural University, Key Laboratory for Agro-Microbial Resource and Application, Ministry of Agriculture, Beijing, 100094, P. R. China.
Zeng
J
J
Department of Microbiology, College of Biological Sciences, China Agricultural University, Key Laboratory for Agro-Microbial Resource and Application, Ministry of Agriculture, Beijing, 100094, P. R. China.
Wang
L
L
Department of Microbiology, College of Biological Sciences, China Agricultural University, Key Laboratory for Agro-Microbial Resource and Application, Ministry of Agriculture, Beijing, 100094, P. R. China.
Dou
Y T
YT
Department of Microbiology, College of Biological Sciences, China Agricultural University, Key Laboratory for Agro-Microbial Resource and Application, Ministry of Agriculture, Beijing, 100094, P. R. China.
Yang
S S
SS
Department of Microbiology, College of Biological Sciences, China Agricultural University, Key Laboratory for Agro-Microbial Resource and Application, Ministry of Agriculture, Beijing, 100094, P. R. China.
D001406:Bacillaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002681:China; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride