title: Effects of N-acetylcysteine, quercetin, and phytic acid on spontaneous hepatic and renal lesions in LEC rats.
abstract: The effects of anti-oxidants were examined in Long-Evans Cinnamon (LEC) rats, which develop acute hepatic injury, and subsequent hepatic and renal tumors due to accumulation of excess Cu. The rats, at the age of 15 weeks, were supplied a diet containing either 1% of N-acetylcysteine (NAC), quercetin (QC), or phytic acid (PA), or basal diet alone. At weeks 2 and 6 posttreatment, animals were sacrificed for collection of blood and tissue samples. In the NAC-treated group, the development of hepatic and renal lesions was dramatically reduced. In addition, accumulation of Cu and Fe in the liver was suppressed. Acrolein-modified protein, a new marker for lipid peroxidation, was not detected in the liver or kidney of NAC treated rats, even though deposition was evident in control. Neither QC nor PA affected the development of spontaneous hepatic lesions. These results indicate that oxidative stress was reduced by NAC in the liver and kidney, and suggest that Cu and Fe may be involved in the generation of oxidative stress in the liver. In addition, it was suggested that the different effects of the anti-oxidants on lesion development in LEC rats might be related to different mechanisms of action with regard to oxidative stress.
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Nishikawa
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Fumio
authors.initials: F
authors.lastname: Furukawa
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Imazawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Umemura
authors.forename: Koji
authors.initials: K
authors.lastname: Uchida
authors.forename: Masao
authors.initials: M
authors.lastname: Hirose
mesh_terms: D000111:Acetylcysteine / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects; D010833:Phytic Acid / Q000737:chemistry / Q000494:pharmacology*; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D020332:Rats, Inbred LEC; D013997:Time Factors
chemical_list: D000975:Antioxidants; D010833:Phytic Acid; D011794:Quercetin; D000111:Acetylcysteine