Ability of some plant extracts, traditionally used to treat ciguatera fish poisoning, to prevent the in vitro neurotoxicity produced by sodium channel activators.
The effects of 31 plant extracts, which most are traditionally used to treat ciguatera fish poisoning in the Pacific area, were studied on the cytotoxicity of mouse neuroblastoma cells produced by ouabain, veratridine and/or brevetoxin-3 or Pacific ciguatoxin-1. The cell viability was determined using a quantitative colorimetric method. A marked cytotoxicity of seven of the 31 plant extracts studied, was observed. Despite this, these plant extracts were suspected to contain active compound(s) against the cytotoxicity produced by brevetoxin (2 extracts), brevetoxin, ouabain and/or veratridine (3 extracts), or only against that of ouabain and/or veratridine (2 extracts). Among the 24 plant extracts that exhibited by themselves no cytotoxicity, 22 were active against the effect of brevetoxin or against that of both veratridine and brevetoxin. Similar results were obtained when the seven most active plant extracts were reassayed using ciguatoxin instead of brevetoxin. In conclusion, the present work reports the first activity assessment of some plant extracts, achieved in vitro on a quite large scale. The fact that 27 plant extracts were found to exert, in vitro, a protective effect against the action of ciguatoxin and/or brevetoxin, paves the way for finding new active compounds to treat ciguatera fish poisoning, provided these compounds also reverse the effects of sodium channel activators.
Boydron-Le Garrec
Raphaële
R
Laboratoire de Pharmacochimie des Substances Naturelles et Pharmacophores Redox, UMR 152, IRD-Université Paul Sabatier, Centre IRD de Nouméa, BP A5, 98848 Nouméa, New Caledonia.
Benoit
Evelyne
E
Sauviat
Martin-Pierre
MP
Lewis
Richard J
RJ
Molgó
Jordi
J
Laurent
Dominique
D
D000818:Animals; D001681:Biological Assay; D045744:Cell Line, Tumor; D002922:Ciguatoxins / Q000037:antagonists & inhibitors* / Q000633:toxicity; D003124:Colorimetry; D003601:Cytotoxicity Tests, Immunologic; D008387:Marine Toxins / Q000037:antagonists & inhibitors* / Q000633:toxicity; D051379:Mice; D010042:Ouabain / Q000037:antagonists & inhibitors* / Q000633:toxicity; D035964:Oxocins / Q000037:antagonists & inhibitors* / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*; D062687:Sodium Channel Agonists; D015222:Sodium Channels / Q000378:metabolism; D013045:Species Specificity; D014701:Veratridine / Q000037:antagonists & inhibitors* / Q000633:toxicity; D000095702:Polyether Toxins
D002922:Ciguatoxins; D008387:Marine Toxins; D010042:Ouabain; D035964:Oxocins; D010936:Plant Extracts; D062687:Sodium Channel Agonists; D015222:Sodium Channels; D014701:Veratridine; C502852:brevetoxin 3, Karenia brevis; D000095702:Polyether Toxins