Identification of self-incompatibility genotypes of apricot (Prunus armeniaca L.) by S-allele-specific PCR analysis.
A cDNA of 417 bp encoding an S-RNase gene, named PA S3, was isolated from apricot, Prunus aremeniaca. Nine S-alleles, S1-S9, were recognized by S-allele-specific PCR and confirmed by Southern blot analysis using PA S3 as probe. The S-genotypes of the six cultivars were determined and the results of self- and cross-pollination tests among the six cultivars were consistent with the predicted S-haplotypes by PCR analysis.
Jie
Qi
Q
College of Horticulture, Shandong Agriculture University, 271018, Taian, China.
Shupeng
Gai
G
Jixiang
Zhang
Z
Manru
Gu
G
Huairui
Shu
S
D000483:Alleles; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D027861:Prunus / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D012098:Reproduction / Q000502:physiology; D012260:Ribonucleases / Q000235:genetics*
D012260:Ribonucleases