[Study on extraction of coumarins in Cnidium monieri by supercritical CO2 and separation of compositions analysis].
OBJECTIVE
To analyze the chemical constituents of supercritical carbon dioxide extraction products from Cnidium monieri.


METHODS
Four-factor and three-level orthogonal experimental design was used to optimize the SFE conditions as guided by the content of total coumarins in the extract. The chemical constituents were separated and identified by recrystalization.


RESULTS
Optimum extraction process was established: 25 MPa as extraction pressure, 50 degrees C as extraction temperature, 6.5 MPa as separation pressure and 60 degrees C as separation temperature.


CONCLUSIONS
Changes in extraction pressure, temperature, time, pulverized degree and separation pressure affect the extracting results remarkably. The two kinds of chemical constituents were separated by recrystallization from C. monieri and identified by the methods of UV, IR, MS, NMR.
Mi
Hong
H
Jilin University, Changchun 130021, China.
Qu
Li-Li
LL
Ren
Yu-Lin
YL
D025924:Chromatography, Supercritical Fluid; D031048:Cnidium / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry; D011564:Furocoumarins / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*
D003374:Coumarins; D011564:Furocoumarins; C031534:imperatorin; C046627:osthol