title: Rapamycin control of transgene expression from a single AAV vector in mouse salivary glands.
abstract: Salivary glands (SGs) appear to be a useful target site for gene therapeutics. The ability to control transgene expression is essential for clinical application. Previously, in a proof-of-concept study, we have shown that the rapamycin-inducible transcriptional regulation system can regulate protein expression after adenoviral-mediated gene transfer to SGs. To evaluate the potential ability to utilize this regulatory system for long-term control of transgene expression in this tissue, we employed a 'third generation', single adenoassociated serotype 2 viral (AAV2) vector encoding human erythropoietin (hEPO) under the control of a rapamycin-inducible promoter. The vector, rAAV-TF2.3-hEPO (10(10) particles/animal), was delivered to mouse SGs. No detectable increase in serum hEPO or hematocrit levels was observed in the absence of rapamycin administration. However, rapamycin induced elevation of serum hEPO levels, as well as concomitant hematocrit changes, that were dose-dependent, completely reversible, and relatively stable over the course of this study (6 months), with no appreciable change in rapamycin responsiveness. Our results suggest that the rapamycin transcriptional regulation system delivered in a single AAV2 vector to SGs may be valuable for systemic protein replacement applications.
authors.affiliation: Gene Therapy and Therapeutics Branch, NIDCR, NIH, DHHS, Bethesda, MD 20892, USA.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: A
authors.initials: A
authors.lastname: Voutetakis
authors.forename: M
authors.initials: M
authors.lastname: Papa
authors.forename: V M
authors.initials: VM
authors.lastname: Rivera
authors.forename: T
authors.initials: T
authors.lastname: Clackson
authors.forename: B M
authors.initials: BM
authors.lastname: Lodde
authors.forename: F
authors.initials: F
authors.lastname: Mineshiba
authors.forename: B J
authors.initials: BJ
authors.lastname: Baum
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004921:Erythropoietin / Q000097:blood / Q000235:genetics* / Q000493:pharmacokinetics; D015870:Gene Expression; D005786:Gene Expression Regulation; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000235:genetics; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D012469:Salivary Glands / Q000378:metabolism*; D020123:Sirolimus / Q000627:therapeutic use*; D013997:Time Factors; D014161:Transduction, Genetic; D019076:Transgenes
chemical_list: D007166:Immunosuppressive Agents; D004921:Erythropoietin; D020123:Sirolimus