Structural characterization of the carbohydrate backbone of the lipooligosaccharide of the marine bacterium Arenibacter certesii strain KMM 3941(T).
The structure of the carbohydrate backbone of the lipooligosaccharide (LOS) of the marine bacterium Arenibacter certesii strain KMM 3941(T) has been elucidated. The structure was obtained by means of compositional analysis, matrix-assisted laser desorption/ionization mass spectrometry and complete 1H and 13C and 31P NMR spectroscopy. It shows novel and interesting aspects and is the first description of Arenibacter lipopolysaccharides. Strong and mild alkaline treatments, to fully deacylate and only to O-deacylate the LOS were performed in order to determine the core structure. The core consists of a mixture of species differing by the presence of a non-stoichiometric alpha-d-rhamnose residue. The Kdo unit is substituted at O-5 by alpha-mannose and at O-4 by a alpha-galactosyluronic acid phosphate. The lipid A is constituted by a bis-phosphorylated disaccharide unit composed by a 2,3-diamino-2,3-dideoxy-beta-d-glucopyranose (DAG) residue as non-reducing end and a GlcN as reducing end.
Silipo
Alba
A
Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, via Cintia 4, 80126 Napoli, Italy.
Molinaro
Antonio
A
Nazarenko
Evgeny L
EL
Sturiale
Luisa
L
Garozzo
Domenico
D
Gorshkova
Raisa P
RP
Nedashkovskaya
Olga I
OI
Lanzetta
Rosa
R
Parrilli
Michelangelo
M
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D041965:Flavobacteriaceae / Q000737:chemistry* / Q000254:growth & development; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008386:Marine Biology; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides