In deuterated water the unspecific adsorption of proteins is significantly slowed down: results of an SPR study using model organic surfaces.
The control of unspecific adsorption of proteins to natural and technical surfaces plays an important role in biology and also for many applications. Organic model surfaces, e.g., self-assembled monolayers, are often used to identify fundamental surface and/or protein properties that rule protein adsorption. Some techniques involved in biointerface research require the use of heavy water, e.g. neutron scattering techniques. Also in NMR studies D(2)O is the solvent of choice when focusing on biomolecular and hydration dynamics. So far several studies have been concerned with the characterization of the unspecific adsorption of proteins from normal water buffers. In the present work, we report a comparison of the unspecific protein adsorption from normal and heavy water buffers. So far it has been assumed that the surface kinetic of the unspecific adsorption is unaffected by the substitution of water by D(2)O. However, for the four proteins investigated here, this assumption does not hold. The ratio k(H)/k(D) of the adsorption rate constants of the different buffer conditions describe the strength of the isotope effect. We have measured ratios between 1.0 and 2.6, indicating that the adsorption kinetics are strongly affected by a H(2)O-D(2)O substitution.
Grunwald
Christian
C
Physikalische Chemie I, Ruhr-Universität, Bochum, Germany, and Max-Planck-Institut für Molekulare Physiologie, Dortmund, Germany.
Kuhlmann
Jürgen
J
Wöll
Christof
C
D000327:Adsorption; D017666:Deuterium Oxide / Q000737:chemistry*; D005982:Glutathione Transferase / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013997:Time Factors; D014867:Water / Q000737:chemistry*
D011506:Proteins; D014867:Water; D012710:Serum Albumin, Bovine; D005982:Glutathione Transferase; D012260:Ribonucleases; D017666:Deuterium Oxide