Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast.
Two rounds of chromosome segregation after only a single round of DNA replication enable the production of haploid gametes from diploid precursors during meiosis. To identify genes involved in meiotic chromosome segregation, we developed an efficient strategy to knock out genes in the fission yeast on a large scale. We used this technique to delete 180 functionally uncharacterized genes whose expression is upregulated during meiosis. Deletion of two genes, sgo1 and mde2, caused massive chromosome missegregation. sgo1 is required for retention of centromeric sister-chromatid cohesion after anaphase I. We show here that mde2 is required for formation of the double-strand breaks necessary for meiotic recombination.
Gregan
Juraj
J
Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
Rabitsch
Peter K
PK
Sakem
Benjamin
B
Csutak
Ortansa
O
Latypov
Vitaly
V
Lehmann
Elisabeth
E
Kohli
Juerg
J
Nasmyth
Kim
K
D002842:Chromatids / Q000235:genetics / Q000502:physiology; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D020090:Chromosome Segregation / Q000235:genetics*; D017931:DNA Primers; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006921:Hygromycin B; D008540:Meiosis / Q000235:genetics*; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013309:Streptothricins
D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers; D051858:Forkhead Transcription Factors; D029702:Schizosaccharomyces pombe Proteins; C485973:Sgo1 protein, S pombe; C509497:mde2 protein, S. pombe; D049452:Green Fluorescent Proteins; D006921:Hygromycin B; D013309:Streptothricins