title: Synthesis and study of molecular interactions between phosphatidyl choline and two laminin derived peptides hydrophobically modified.
abstract: Two peptides based on the active SIKVAV sequence were synthesized and hydrophobically modified through attachment of a myristoyl residue to the amino terminal group. A comparative study was carried out on the physicochemical properties of both parent and hydrophobically modified structures. Properties studied were hydrophobicity, surface activity, spreadability on aqueous surfaces, penetration in lipid monolayers, aggregation and haemolytic activity. Results obtained indicate that myristoyl containing peptides form micelles at 10(-6) M concentration, whereas parent peptides start to aggregate at 10(-5) M. All of them are able to spread on aqueous surfaces forming stable monolayers. Concerning their haemolytic activity only one of the sequences promotes a partial lysis of erythrocytes after 30 min incubation at 37 degrees C and 10(-5) M concentration in the media.
authors.affiliation: Physicochemistry Department, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII s/n, 08028 Barcelona, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Alsina
authors.forename: A
authors.initials: A
authors.lastname: Ortiz
authors.forename: D
authors.initials: D
authors.lastname: Polo
authors.forename: F
authors.initials: F
authors.lastname: Comelles
authors.forename: F
authors.initials: F
authors.lastname: Reig
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D007797:Laminin / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D008823:Micelles; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D013499:Surface Properties
chemical_list: D007797:Laminin; D008055:Lipids; D008823:Micelles; D010455:Peptides; D010713:Phosphatidylcholines; D010743:Phospholipids