title: [Preparation and characterization of monoclonal antibodies against erythrocyte-binding antigen 175 of Plasmodium falciparum].
abstract: OBJECTIVE
  To prepare and characterize the monoclonal antibodies (mAbs) against erythrocyte-binding antigen 175 of Plasmodium falciparum (EBA-175).
  
  
  METHODS
  BALB/ c mice were immunized with purified recombinant EBA-175 and mAbs against EBA-175 were prepared by means of hybridoma technique. Enzyme-linked immunosorbent assay (ELISA) and Western blotting were employed for characterization of the mAbs.
  
  
  RESULTS
  Six McAbs against EBA-175 antigen were obtained, 5 of which were identified as IgG1 and one as IgG2a. The titer of these aAbs was 1:12,800 to 1:25,600 in the ascites and 1:256 to 1:512 in supernatant, and ELISA demonstrated specific binding of the 4 mAbs (1F3, 2H5, 4A1 and 4H9)with Plasmodium falciparum. Three of these mAbs recognized the protein of EBA-175 as shown by Western blotting.
  
  
  CONCLUSIONS
  Six hybridoma cell lines secreting the mAbs against EBA175 with high specificity are successfully established.
authors.affiliation: School of Biotechnology, Southern Medical University, Guangzhou 510515, China. haowa@126.com
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Hao
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Hong
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Sun
authors.forename: Yunshan
authors.initials: Y
authors.lastname: Ning
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Zhang
authors.forename: Wei-qing
authors.initials: WQ
authors.lastname: Pan
authors.forename: Ming
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C065668:erythrocyte-binding antigen 175, Plasmodium