DNA biosensor based on chitosan film doped with carbon nanotubes.
A biosensor based on chitosan doped with carbon nanotube (CNT) was fabricated to detect salmon sperm DNA. Methylene blue (MB) was employed as a DNA indicator. It was found that CNTs can enhance the electroactive surface area threefold (0.28 +/- 0.03 and 0.093 +/- 0.06 cm(2) for chitosan-CNT- and chitosan-modified electrodes, respectively) and can accelerate the rate of electron transfer between the redox-active MB and the electrode. A low detection limit of 0.252 nM fish sperm DNA was achieved, and no interference was found in the presence of 5 microg/ml human serum albumin. The differential pulse voltammetry signal of MB was linear over the fish sperm DNA concentration range of 0.5-20 nM.
Li
Jia
J
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China.
Liu
Qian
Q
Liu
Yingju
Y
Liu
Shanchao
S
Yao
Shouzhuo
S
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D008751:Methylene Blue; D037742:Nanotubes, Carbon; D012474:Salmon
D037742:Nanotubes, Carbon; D004247:DNA; D048271:Chitosan; D008751:Methylene Blue