title: Rapid identification of Candida tropicalis from canine cystitis.
abstract: The isolate from urine of a dog with cystitis was molecularly identified Candida tropicalis and its minimum inhibitory concentration (MIC) was determined by a microdilution method. The 25S ribosomal DNA sequence analysis indicated that the clinical isolate was essentially identical to that of C. tropicalis and distinct from other Candida species. The MIC(50) and the MIC(90) of fluconazole (FLZ) for the clinical isolate of C. tropicalis was 6.25 and 25 microg/ml, respectively, indicating that susceptibility of the clinical isolate of C. tropicalis to FLZ was less than for other strains of C. tropicalis as well as C. albicans. The molecular analysis as presented in this study assisted the diagnosis of candidiasis by identifying the yeasts in urine samples within 2 days. The patient dog, a 10-year-old male Shih Tzu dog (7.0 kg) referred for examination of cystitis was successfully treated with itraconazole.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, Kameino, Kanagawa Perfecture, Fujisawa, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ozawa
authors.forename: Ken
authors.initials: K
authors.lastname: Okabayashi
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Watari
authors.forename: Shinichi
authors.initials: S
authors.lastname: Watanabe
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology / Q000627:therapeutic use; D041022:Candida tropicalis / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology / Q000662:veterinary*; D003556:Cystitis / Q000382:microbiology / Q000662:veterinary*; D004271:DNA, Fungal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D017964:Itraconazole / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D008826:Microbial Sensitivity Tests; D016533:Mycological Typing Techniques; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014556:Urine / Q000382:microbiology
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065039:RNA, ribosomal, 25S; D017964:Itraconazole