title: Nosocomial urosepsis caused by Enterobacter kobei with aberrant phenotype.
abstract: Enterobacter kobei is the species of the Enterobacter cloacae complex, which is phenotypically most closely related to the species E. cloacae. This is the first report of infection caused by a new biotype of E. kobei. A patient with a history of urinary bladder operation developed a urosepsis with an Enterobacter isolate displaying the E. cloacae phenotype. The isolate was classified to the species E. kobei by sequence analysis of the 16S-rDNA, 4 protein-coding genes and enterobacterial repetitive intergenic consensus (ERIC)-cluster analysis. E. kobei was originally described to be Voges-Proskauer (VP) negative. However, the isolates of the present case were VP-positive. After analyzing 120 biochemical tests included in the API20E and the Biotype 100 systems, 4 biochemical tests were identified potentially differentiating this new biotype from E. cloacae.
authors.affiliation: Institute of Microbiology and Laboratory Medicine, Pneumological Teaching Hospital of the Ludwig-Maximilians-University Munich, Robert-Koch-Allee 2, D-82131 Gauting, Germany. harald.hoffmann@asklepios.com
authors.forename: Harald
authors.initials: H
authors.lastname: Hoffmann
authors.forename: Sabine
authors.initials: S
authors.lastname: Schmoldt
authors.forename: Konrad
authors.initials: K
authors.lastname: Trülzsch
authors.forename: Anita
authors.initials: A
authors.lastname: Stumpf
authors.forename: Stefan
authors.initials: S
authors.lastname: Bengsch
authors.forename: Thomas
authors.initials: T
authors.lastname: Blankenstein
authors.forename: Jürgen
authors.initials: J
authors.lastname: Heesemann
authors.forename: Andreas
authors.initials: A
authors.lastname: Roggenkamp
mesh_terms: D003428:Cross Infection / Q000382:microbiology* / Q000635:transmission; D016972:Enterobacter cloacae / Q000145:classification* / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000382:microbiology* / Q000635:transmission; D005260:Female; D006801:Humans; D008875:Middle Aged; D018307:Neoplasms, Squamous Cell / Q000150:complications; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D012336:RNA, Ribosomal, 16S