title: Live lambs born from zona-pellucida denuded embryos.
abstract: Progress with techniques using zona-pellucida denuded embryos has resulted in the birth of live cattle, pigs, and mice. The application of zona-free methods in sheep has been restricted to in vitro studies. In this report, we demonstrate that live lambs can be produced from zona-free IVF embryos. We are pursuing this method as a prerequisite to developing viral vector co-culture delivery strategies.
authors.affiliation: Department of Gene Function and Development, Roslin Institute, Roslin, Midlothian, United Kingdom. william.ritchie@bbsrc.ac.uk
authors.forename: William A
authors.initials: WA
authors.lastname: Ritchie
authors.forename: Jane E
authors.initials: JE
authors.lastname: Taylor
authors.forename: John O
authors.initials: JO
authors.lastname: Gardner
authors.forename: Ian
authors.initials: I
authors.lastname: Wilmut
authors.forename: Ailsa
authors.initials: A
authors.lastname: Carlisle
authors.forename: Claire
authors.initials: C
authors.lastname: Neil
authors.forename: Tim
authors.initials: T
authors.lastname: King
authors.forename: C Bruce A
authors.initials: CB
authors.lastname: Whitelaw
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D011247:Pregnancy; D012756:Sheep; D015044:Zona Pellucida / Q000502:physiology