title: Potential marker for smoked methamphetamine hydrochloride based on a gas chromatography-mass spectrometry quantification method for trans-phenylpropene.
abstract: Residue from smoked methamphetamine hydrochloride contains pyrolytic products that are detectable by gas chromatography-mass spectrometry (GC-MS). A validated GC-MS method was developed for the determination of trans-phenylpropene, a pyrolytic product of methamphetamine HCl, in residue of smoked drug as well as in human urine. trans-Phenylpropene and an isomeric internal standard, 2-phenylpropene, were extracted from urine using n-hexane. The method was validated for linearity over a range of 0.1-10 microg/mL with a limit of detection of 0.05 microg/mL, limit of quantification of 0.1 microg/mL, interday accuracy within 10.5%, intraday accuracy better than 3.7%, interday precision of 15.4%, intraday precision of 14.4%, and recovery of 89.1%. The method was applied to the detection of trans-phenylpropene found in the residue of methamphetamine HCl heated beyond its melting temperature on aluminum foil under simulated smoking conditions. The method is applicable to the detection of trans-phenylpropene in urine as a potential marker for smoked methamphetamine HCl abuse.
authors.affiliation: Basic Pharmaceutical Sciences, West Virginia University, P.O. Box 9530, Morgantown, West Virginia 26506, USA.
authors.forename: Diaa M
authors.initials: DM
authors.lastname: Shakleya
authors.forename: Sandra G
authors.initials: SG
authors.lastname: Tarr
authors.forename: James C
authors.initials: JC
authors.lastname: Kraner
authors.forename: David J
authors.initials: DJ
authors.lastname: Clay
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Callery
mesh_terms: D015415:Biomarkers / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008694:Methamphetamine / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012906:Smoke / Q000032:analysis*; D013343:Styrenes / Q000652:urine*; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D015415:Biomarkers; D012906:Smoke; D013343:Styrenes; D008694:Methamphetamine; C505958:1-phenylpropene