Development of a multicomponent method for Fusarium toxins using LC-MS/MS and its application during a survey for the content of T-2 toxin and deoxynivalenol in various feed and food samples.
A reliable, sensitive and selective method was developed to determine different Fusarium mycotoxins (trichothecenes Type A and B, zearalenone) simultaneously in cereals and cereal-based samples using liquid chromatography with tandem mass spectrometry (LC-ESI-MS/MS). Sample preparation is based on a standard solvent extraction step followed by two different kinds of solid-phase clean-up procedures: using a multifunctional MycoSep material for trichothecenes and zearalenone. The average recoveries for trichothecenes ranged from 65% for nivalenol (NIV) up to 96% for deoxynivalenol (DON) and 89% for zearalenone (ZON). The limit of quantification varied between 0.02 and 10 ppb for each substance. In addition, a screening survey with 685 samples was carried out to compare contents of T-2 toxin and deoxynivalenol and to investigate potential coherence in contamination pattern.
Biselli
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S
Eurofins/Wiertz-Eggert-Förissen, Hamburg, Germany. Scarlett.Biselli@wej.de
Hummert
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C
D002853:Chromatography, Liquid / Q000379:methods; D002523:Edible Grain / Q000737:chemistry*; D005433:Flour / Q000032:analysis; D005527:Food, Fortified; D005670:Fusarium / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D013605:T-2 Toxin / Q000031:analogs & derivatives / Q000032:analysis; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000737:chemistry; D015025:Zearalenone / Q000031:analogs & derivatives / Q000032:analysis*
D014255:Trichothecenes; C011140:verrucarol; C002469:fusarenon-X; C038972:acetyldeoxynivalenol; D015025:Zearalenone; C038405:nivalenol; D013605:T-2 Toxin; C007262:deoxynivalenol; C012351:HT-2 toxin; C001809:diacetoxyscirpenol