The effects of oridonin on cell growth, cell cycle, cell migration and differentiation in melanoma cells.
Oridonin, a diterpenoid isolated from Rabdosia rubescens (Hemsl.) Hara, is currently one of the most important traditional Chinese herbal medicines. We investigated the anti-proliferation effect of oridonin on the cultured murine melanoma cell line K1735M2. The growth inhibition activity of oridonin for K1735M2 cells occurred in a time- and dose-dependent manner (IC50 was 7.4+/-0.6 microM). Further studies showed that these inhibition effects were associated with dose-dependent G2/M phase arrest and differentiation induction. Detection of morphological observation showed that oridonin could induce K1735M2 cells to produce dendrite-like structures. The results of the migration indicated that oridonin affected motility of K1735M2 cells in a dose-dependent manner. These results suggested that oridonin is a potential candidate for melanoma cancer therapy.
Ren
Kui-Kui
KK
Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China.
Wang
Hong-Zhong
HZ
Xie
Li-Ping
LP
Chen
Da-Wei
DW
Liu
Xing
X
Sun
Juan
J
Nie
Yan-Cheng
YC
Zhang
Rong-Qing
RQ
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D004224:Diterpenes / Q000302:isolation & purification / Q000627:therapeutic use*; D045786:Diterpenes, Kaurane; D006801:Humans; D037602:Isodon; D008546:Melanoma, Experimental / Q000188:drug therapy*; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
D004224:Diterpenes; D045786:Diterpenes, Kaurane; D010936:Plant Extracts; C011959:oridonin