Flow injection fluorimetric determination of beta-estradiol using a molecularly imprinted polymer.
The steroidal compound 17beta-estradiol (E2) is a major estrogen now widely studied because of its potential endocrine disruption effects. The present paper describes a simple, rapid, flow injection (FI) method for the fluorimetric determination of beta-estradiol involving a molecularly imprinted polymer (MIP) packed into a microcolumn. Microwave-assisted extraction, a relatively new technique, was used to help remove the template from the MIP; this improved extraction efficiency. The optimization of the FI parameters and the intrinsic fluorescence of beta-estradiol allowed its direct determination without a derivatization step. When the possible interference of other estrogens was examined, the system displayed excellent specificity for beta-estradiol; no response to the natural estrogens estrone and estriol and the xenoestrogen bisphenol A was recorded. Under optimum experimental conditions, linear calibration graphs were obtained from 4 to 80 microg L(-1) with a detection limit of 1.12 microg L(-1).
Bravo
Juan C
JC
Departamento de Ciencias Analíticas, Facultad de Ciencias, Universidad Nacional de Educación a Distancia, 28040, Madrid, Spain.
Fernández
Pilar
P
Durand
Jesús S
JS
D004337:Drug Carriers; D004958:Estradiol / Q000032:analysis*; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D005470:Fluorometry / Q000379:methods; D006801:Humans; D008832:Microchemistry; D011108:Polymers; D014874:Water Pollutants, Chemical / Q000032:analysis*
D004337:Drug Carriers; D011108:Polymers; D014874:Water Pollutants, Chemical; D004958:Estradiol