title: Blunted inflammatory response to lipopolysaccharide endotracheal instillation due to thermal preconditioning.
abstract: OBJECTIVE
  Heat shock proteins (HSPs), induced by thermal stress, make the cells more resistant to following stress. We aimed to verify if rats heating reduces the production of TNF-alpha in a model of acute respiratory distress syndrome (ARDS).
  
  
  METHODS
  We divided 34 Wistar rats in Group A and Group B. The rats of Group A received thermal shock and tracheal instillation of lipopolysaccharide (LPS), the rats of Group B only tracheal instillation of LPS. All the rats have been then subdivided in 6 subgroups sacrificed at different times after instillation of LPS: A30 and B30 at 30 min, A90 and B90 at 90 min and A180 and B180 at 180 min. We dosed the serum concentration of TNF-alpha and the values of every subgroup have been compared with the Student t-test.
  
  
  RESULTS
  The serum concentration of TNF-alpha in the subgroup A90 resulted significantly lower than in the subgroup B90.
  
  
  CONCLUSIONS
  The exposure to thermal stress resulting in the production of HSPs reduced the concentration of TNF-alpha in this model of ARDS.
authors.affiliation: Section of Anaesthesia, Department of Critical Care, University of Florence, Viale Morgagni 85, 50124 Florence, Italy. aledanibatibeba@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Di Filippo
authors.forename: S
authors.initials: S
authors.lastname: Rinaldi
authors.forename: C
authors.initials: C
authors.lastname: Pascente
authors.forename: D
authors.initials: D
authors.lastname: Messeri
authors.forename: A R
authors.initials: AR
authors.lastname: De Gaudio
mesh_terms: D000818:Animals; D001831:Body Temperature; D004195:Disease Models, Animal; D006358:Hot Temperature; D007322:Instillation, Drug; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012128:Respiratory Distress Syndrome / Q000276:immunology; D014132:Trachea; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000187:drug effects
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha