Hairy root cultures of Gynostemma pentaphyllum (Thunb.) Makino: a promising approach for the production of gypenosides as an alternative of ginseng saponins.
Hairy root cultures of Gynostemma pentaphyllum were established by infecting leaf discs with Agrobacterium rhizogenes. The dry biomass of hairy roots grown in MS medium for 49 days was 7.3 g l(-1) with a gypenoside content of 38 mg g(-1) dry wt.
Chang
Chen-Kai
CK
Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan.
Chang
Ku-Shang
KS
Lin
Yi-Chen
YC
Liu
Sin-Yie
SY
Chen
Chien-Yuan
CY
D003001:Cloning, Molecular / Q000379:methods*; D029774:Gynostemma / Q000235:genetics* / Q000378:metabolism*; D005894:Panax / Q000378:metabolism; D010936:Plant Extracts / Q000096:biosynthesis; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012503:Saponins / Q000096:biosynthesis; D014162:Transfection / Q000379:methods*
D010936:Plant Extracts; D011994:Recombinant Proteins; D012503:Saponins; C474918:gypenoside