title: Chromosome abnormalities in 10 lung cancer cell lines of the NCI-H series analyzed with spectral karyotyping.
abstract: The karyotypes of 10 lung cancer cell lines of the NCI-H series were analyzed with spectral karyotyping (SKY): 7 non-small lung cancer (NSCLC) lines and 3 small cell lung cancer (SCLC) lines. Modal chromosome number ranged from 42 (NCI-H2171) to 72 (NCI-H2126). All lines showed at least six structural abnormalities, and most had amplifications visible as double minutes or homogeneously staining regions (HSRs). Four reciprocal translocations were found: t(1;17)(p10;p10) in NCI-H82, t(3;6)(q24;q21) and t(12;17)(p10;p10) in NCI-H2009, and a complex t(2;6) in NCI-H1437. NCI-H1770 had a striking HSR containing many copies of the NMYC region. Karyotypes showed a wide range of relationship between numerical and structural change. Two of the lines showed little numerical change but many structural rearrangements (NCI-H209 with mode 46, but 12 rearrangements, and NCI-H2009 with mode 48 but 27 rearrangements). A second group had karyotypes that appeared to have evolved by unbalanced translocation leading to proportionate loss of chromosomes, with or without endoreduplication. In other lines, notably NCI-H2122, the structurally abnormal chromosomes appeared to have been added to a near-diploid karyotype. The karyotypes contribute to a full genomic characterization of these lines, almost all of which have matching normal lymphoblastoid cell lines.
authors.affiliation: Cancer Genomics Program, Hutchison-MRC Research Centre, Departments of Pathology and Oncology, University of Cambridge, Hills Road, Cambridge CB2 2XZ, UK.
authors.forename: Mira
authors.initials: M
authors.lastname: Grigorova
authors.forename: Rachel C
authors.initials: RC
authors.lastname: Lyman
authors.forename: Carlos
authors.initials: C
authors.lastname: Caldas
authors.forename: Paul A W
authors.initials: PA
authors.lastname: Edwards
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002869:Chromosome Aberrations; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D032681:Spectral Karyotyping; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured