title: Crk-associated substrate lymphocyte type is required for lymphocyte trafficking and marginal zone B cell maintenance.
abstract: The lymphocyte-specific Cas family protein Cas-L (Crk-associated substrate lymphocyte type) has been implicated to function in lymphocyte movement, mediated mainly by integrin signaling. However, its physiological role is poorly understood. In this study we analyzed the function of Cas-L in lymphocytes using gene-targeted mice. The mutant mice showed a deficit of marginal zone B (MZB) cells and a decrease of cell number in secondary lymphoid organs. An insufficient chemotactic response and perturbed cell adhesion were observed in Cas-L-deficient lymphocytes, suggesting that the aberrant localization was responsible for the deficit of MZB cells. Moreover, we found that lymphocyte trafficking was altered in Cas-L-deficient mice, which gave a potential reason for contraction of secondary lymphoid tissues. Thus, Cas-L affects homeostasis of MZB cells and peripheral lymphoid organs, which is considered to be relevant to impaired lymphocyte migration and adhesion.
authors.affiliation: Department of Hematology and Oncology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Seo
authors.forename: Takashi
authors.initials: T
authors.lastname: Asai
authors.forename: Toshiki
authors.initials: T
authors.lastname: Saito
authors.forename: Takahiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Morishita
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakamoto
authors.forename: Motoshi
authors.initials: M
authors.lastname: Ichikawa
authors.forename: Go
authors.initials: G
authors.lastname: Yamamoto
authors.forename: Masahito
authors.initials: M
authors.lastname: Kawazu
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamagata
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Sakai
authors.forename: Kinuko
authors.initials: K
authors.lastname: Mitani
authors.forename: Seishi
authors.initials: S
authors.lastname: Ogawa
authors.forename: Mineo
authors.initials: M
authors.lastname: Kurokawa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Chiba
authors.forename: Hisamaru
authors.initials: H
authors.lastname: Hirai
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002634:Chemotaxis, Leukocyte; D006706:Homeostasis; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology; D008221:Lymphoid Tissue / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011506:Proteins / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C505592:NEDD9 protein, mouse; D011506:Proteins