Rapid identification of Lactobacillus acidophilus by restriction analysis of the 16S-23S rRNA intergenic spacer region and flanking 23S rRNA gene.
A rapid molecular approach was developed for the initial identification of Lactobacillus acidophilus strains which are difficult to identify using a single biochemical test. The 16S-23S rRNA intergenic spacer regions and flanking 23S rRNA genes of 19 strains of lactobacilli were amplified and the nucleotide sequences and restriction site polymorphisms were analyzed. AluI was the most useful of the restriction enzymes analyzed and produced reproducible digestion profiles in the L. helveticus, L. plantarum, and L. casei groups, as well as in L. acidophilus. This restriction fragment length polymorphism method may be useful for the identification of L. acidophilus strains in dairy products.
Han
Kyoung-Sik
KS
Division of Food Science, Korea University, 136-701, Seoul, Korea.
Kim
Younghoon
Y
Choi
Seungkook
S
Oh
Sejong
S
Park
Sungsu
S
Kim
Sae-Hun
SH
Whang
Kwang-Youn
KY
D024509:3' Flanking Region / Q000235:genetics; D015169:Colony Count, Microbial / Q000379:methods*; D021901:DNA, Intergenic / Q000235:genetics; D007779:Lactobacillus acidophilus / Q000235:genetics* / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D015183:Restriction Mapping / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods*
D021901:DNA, Intergenic; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S