title: Optical nanoinjection of macromolecules into vital cells.
abstract: A tuneable near infrared 90MHz mode locked femtosecond laser was applied for targeted multiphoton optoporation of vital cells. Here, we demonstrate the laser assisted transfer of large amounts of exogenous materials and even macromolecules into living cells via a transient opening of the membrane and quantify the influx. The use of near infrared lasers also allow the optoporation of cells deep in three dimensional biological structures without photodestructive collateral effects.
authors.affiliation: Fraunhofer Institute for Biomedical Technology (IBMT), Lasermedicine, Ensheimer Street 48, D-66386 St. Ingbert, Germany. frank.stracke@ibmt.fraunhofer.de
authors.forename: Frank
authors.initials: F
authors.lastname: Stracke
authors.forename: Iris
authors.initials: I
authors.lastname: Rieman
authors.forename: Karsten
authors.initials: K
authors.lastname: König
mesh_terms: D000818:Animals; D016466:CHO Cells; D045744:Cell Line, Tumor; D006224:Cricetinae; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000378:metabolism; D006801:Humans; D007259:Infrared Rays; D007834:Lasers / Q000145:classification*; D046911:Macromolecular Substances / Q000008:administration & dosage* / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D008856:Microscopy, Fluorescence; D051381:Rats; D013050:Spectrometry, Fluorescence
chemical_list: D046911:Macromolecular Substances; D016650:Fluorescein-5-isothiocyanate