Catecholate receptor proteins in Salmonella enterica: role in virulence and implications for vaccine development.
Three outer membrane proteins of Salmonella enterica serovar Typhimurium function as catecholate siderophore receptors. IroN promotes uptake of enterobactin, salmochelins and 2,3-dihydroxybenzoylserine, FepA transports enterobactin and 2,3-dihydroxybenzoylserine, and Cir is a receptor for 2,3-dihydroxybenzoylserine. In addition, all three proteins are required for l-norepinephrine-facilitated iron uptake from transferrin as judged by failure of a fepA iroN cir triple mutant to grow in serum-containing medium in the presence of l-norepinephrine. Moreover, pre-treatment of mice with l-norepinephrine resulted in enhanced systemic spread of the parental strain, but had no effect on the fepA iroN cir mutant. Inoculation of mice with the triple mutant, which is significantly attenuated, elicited a significant protective effect against subsequent challenge with the parental strain.
Williams
P H
PH
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK. phw2@le.ac.uk
Rabsch
W
W
Methner
U
U
Voigt
W
W
Tschäpe
H
H
Reissbrodt
R
R
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000502:physiology*; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology; D002432:Cecum / Q000382:microbiology; D005260:Female; D017353:Gene Deletion; D008099:Liver / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D009638:Norepinephrine / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology; D022562:Salmonella Vaccines; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D044967:Serum / Q000382:microbiology; D016019:Survival Analysis; D037521:Virulence Factors
D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D011956:Receptors, Cell Surface; D022562:Salmonella Vaccines; D037521:Virulence Factors; C029489:enterobactin receptor; D009638:Norepinephrine