A new model system for investigation of ionic channels in filamentous fungi: evidence for existence of two K+-permeable ionic channels in Phycomyces blakesleeanus.
A technique was developed to obtain viable cytoplasmic droplets, enabling the formation of a gigaohm seal. Such cytoplasmic droplets can be used for characterization of plasma membrane ion channels from filamentous fungi by patch-clamp technique. Two K(+) ionic channels are characterized with a conductance of 43 pS and 74 pS.
Zivić
Miroslav
M
Institute of General and Physical Chemistry, Belgrade, Serbia and Montenegro. mzivic@iofh.bg.ac.yu
Popović
Marko
M
Zivanović
Branka
B
Vucinić
Zeljko
Z
D002463:Cell Membrane Permeability / Q000502:physiology*; D003593:Cytoplasm / Q000737:chemistry / Q000648:ultrastructure; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods*; D005658:Fungi / Q000254:growth & development* / Q000648:ultrastructure; D007473:Ion Channels / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D008954:Models, Biological; D018408:Patch-Clamp Techniques; D010800:Phycomyces / Q000502:physiology*; D015221:Potassium Channels / Q000502:physiology
D007473:Ion Channels; D015221:Potassium Channels