Molecular cloning and functional expression of d-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli.
A NADP-dependent d-arabitol dehydrogenase gene was cloned from Gluconobacter oxydans CGMCC 1.110 and functionally expressed in Escherichia coli. With d-arabitol as sole carbon source, E. coli transformants grew rapidly in minimal medium, and produced d-xylulose. The enzymatic properties of the 29kDa enzyme were documented. The DNA sequence surrounding the gene suggested that it is part of an operon with several components of a sugar alcohol transporter system, and the d-arabitol dehydrogenase gene belongs to the short-chain dehydrogenase family.
Cheng
Hairong
H
Center for Microbial Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Jiang
Ning
N
Shen
An
A
Feng
Youjun
Y
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D020600:Gluconobacter oxydans / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013402:Sugar Alcohols / Q000378:metabolism
D003470:Culture Media; D013402:Sugar Alcohols; D009249:NADP; D013401:Sugar Alcohol Dehydrogenases; C022751:D-arabinitol dehydrogenase; C014999:arabitol