Adiponectin is expressed in the brown adipose tissue and surrounding immature tissues in mouse embryos.
Adiponectin is one of the adipocytokines, which are adipose-specific secretory factors. We examined its expression during embryogenesis. Transcripts of adiponectin were detected at a late stage of embryogenesis on embryonic (E) day E16.5. In situ hybridization showed that adiponectin transcripts were localized in brown adipose tissues (BATs) and surrounding immature tissues in mouse embryos. Immunohistochemistry using a specific anti-adiponectin antibody showed that the distribution of adiponectin closely parallels that of its mRNA. Adiponectin was also detected in serum at day E16.5, and its concentration peaked at birth. By contrast, transcripts of both the adiponectin receptors 1 and 2 were already expressed by day E12.5 in many tissues. Thus, their expression profile differed from that of adiponectin itself. Furthermore, experiments using primary cultures of brown adipocytes showed that adiponectin is regulated in brown adipocytes by various modulators, similar to its regulation in white adipose tissues (WATs). These data indicate that adiponectin has important roles in glucose and lipid metabolism during the perinatal period.
Fujimoto
Noritaka
N
Department of Anatomy, Biology and Medicine, Oita University, Faculty of Medicine, 1-1 Hasama-machi, Oita 879-5593, Japan.
Matsuo
Noritaka
N
Sumiyoshi
Hideaki
H
Yamaguchi
Kenji
K
Saikawa
Tetunori
T
Yoshimatsu
Hironobu
H
Yoshioka
Hidekatsu
H
D052242:Adiponectin / Q000096:biosynthesis*; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002839:Chromans / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007328:Insulin / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D011817:Rabbits; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045162:Thiazolidinediones / Q000494:pharmacology; D014284:Triiodothyronine / Q000494:pharmacology; D000077288:Troglitazone
D052242:Adiponectin; D002839:Chromans; D007328:Insulin; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface; D045162:Thiazolidinediones; C475419:adiponectin receptor 1, mouse; C475421:adiponectin receptor 2, mouse; D014284:Triiodothyronine; D003907:Dexamethasone; D000077288:Troglitazone; D007545:Isoproterenol