[Effect of PC-1 gene expression on migration ability of prostate cancer cells].
OBJECTIVE
PC-1 gene is highly expressed in C4-2 cell line, an androgen-independent and aggressive prostate cancer cell line, but lowly expressed in LNCaP cell line, an androgen-dependent prostate cancer cell line. This study was to assess the contribution of PC-1 gene to migration of prostate cancer cells.


METHODS
LNCaP cell line with stable expression of PC-1 gene and C4-2 cell line with antisense nucleotide-down-regulated PC-1 were established. The migration abilities of LNCaP and C4-2 cells were tested using an in vitro transwell invasion assay.


RESULTS
Migration cell count of LNCaP cells with up-regulation of PC-1 gene was similar to that of control LNCaP cells(P>0.05); migration cell count was significantly lower in C4-2 cells with down-regulation of endogenous PC-1 gene than in control C4-2 cells (P<0.05).


CONCLUSIONS
PC-1 may be involved in the invasion of prostate cancer cells.
Zhang
Hui
H
Laboratory of Molecular Oncology, Institute of Biotechnology, Academy of Military Medical Science, Beijing, P.R. China.
Wan
Li-Chuan
LC
Wang
Jian
J
Zhou
Jian-Guang
JG
Huang
Cui-Fen
CF
D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009376:Neoplasms, Hormone-Dependent / Q000378:metabolism* / Q000473:pathology; D010727:Phosphoric Diester Hydrolases / Q000235:genetics*; D010957:Plasmids; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011755:Pyrophosphatases / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins; D010727:Phosphoric Diester Hydrolases; C065962:ectonucleotide pyrophosphatase phosphodiesterase 1; D011755:Pyrophosphatases