Effect of iron restriction on outer membrane protein composition of Pseudomonas strains studied by conventional and microchip electrophoresis.
In this study the outer membrane protein (OMP) composition of six Pseudomonas aeruginosa strains had been analyzed by conventional CE and microchip electrophoresis. Bacterial OMPs are important virulence factors and play a significant role in the pathogenesis of infectious diseases. Changes in their composition might refer to the altered pathogenic properties and antibiotic sensitivity of a certain strain. Pathogenic bacteria invading the human host have to multiplicate under iron-restricted conditions that induce changes in the OMP composition. High-molecular-weight OMPs have to be expressed, which serve as receptors for the iron-siderophore complexes. OMP patterns of bacteria obtained by the two different methods in this study were similar, all major proteins could be detected by both techniques, and the molecular weights showed good correlations, although direct comparison of the peak areas is not straightforward due to the different detection methods (UV and LIF). Changes in OMP composition under iron restriction could be detected, and appearance of a 92 kDa protein in all six P. aeruginosa strains and a 94 kDa protein in the KT 2 strain could be demonstrated. Besides that up- and downregulation of certain proteins could be also detected. The increased separation speed, picoliters of sample consumption, baseline separation achieved more frequently by this method--especially in the high-molecular-weight region--showed the advantages of microchip electrophoresis in the analysis of clinical samples.
Kustos
Ildikó
I
Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Pécs, Hungary. Ildiko.Kustos@aok.pte.hu
Andrásfalvy
Márton
M
Kustos
Tamás
T
Kocsis
Béla
B
Kilár
Ferenc
F
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D004586:Electrophoresis; D046209:Electrophoresis, Microchip; D007501:Iron / Q000502:physiology; D011550:Pseudomonas aeruginosa / Q000737:chemistry*
D001425:Bacterial Outer Membrane Proteins; D007501:Iron