Modulation of CSF-1-regulated post-natal development with anti-CSF-1 antibody.
Colony-stimulating factor-1 (CSF-1) regulates the survival, proliferation and differentiation of macrophages. CSF-1-deficient mice are osteopetrotic due to a lack of osteoclasts, while their tissue macrophage deficiencies and an absence of CSF-1 regulation of CSF-1 receptor-expressing cells in the female reproductive tract contribute to their pleiotropic phenotype. To further understand CSF-1 regulation of macrophages in vivo, we developed a neutralizing anti-mouse CSF-1 antibody which was expressed as a recombinant Fab' fragment and coupled to 40 kDa polyethylene glycol. As developmental regulation by CSF-1 is highest during the early post-natal period, the ability of this anti-CSF-1 reagent to inhibit development was tested by regular subcutaneous injection of mice from post-natal days 0.5-57.5. Antibody treatment decreased growth rate, decreased osteoclast number, induced osteopetrosis, decreased macrophage density in bone marrow, liver, dermis, synovium and kidney and decreased adipocyte size in adipose tissue, thereby inducing phenotypes shared by CSF-1- and CSF-1 receptor-deficient mice. While the antibody blocked macrophage development in some tissues, macrophage densities in other tissues were initially high and were reduced by treatment, proving that the antibody also blocked macrophage maintenance. Since cell surface CSF-1 is sufficient for the maintenance of normal synovial macrophage densities, these studies suggest that anti-CSF-1 Fab'-PEG efficiently neutralizes all three CSF-1 isoforms in vivo, namely the secreted proteoglycan, secreted glycoprotein and cell surface glycoprotein. Since CSF-1 has been shown to enhance chronic disease development in a number of mouse model systems, these studies demonstrate the feasibility of neutralizing CSF-1 effects in these models with an anti-CSF-1 antibody.
Wei
Suwen
S
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Lightwood
Daniel
D
Ladyman
Heather
H
Cross
Sue
S
Neale
Helen
H
Griffiths
Meryn
M
Adams
Ralph
R
Marshall
Diane
D
Lawson
Alastair
A
McKnight
Andrew J
AJ
Stanley
E Richard
ER
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology; D007107:Immune System / Q000187:drug effects / Q000254:growth & development*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism; D010022:Osteopetrosis / Q000209:etiology / Q000473:pathology
D007140:Immunoglobulin Fab Fragments; D016173:Macrophage Colony-Stimulating Factor