Nuclear status of four-cell preembryos predicts implantation potential in in vitro fertilization treatment cycles.
OBJECTIVE
To assess the value of routine screening of nuclear status of day-2 four-cell preembryos for single embryo transfer (SET) in predicting implantation.


METHODS
Retrospective analysis.


METHODS
Private IVF unit.


METHODS
A total of 1,985 fresh embryo transfers on day 2 or day 3 were performed from January 2002 to November 2004. In 1,295 (65.2%) of these transfers, SET was performed. All day-2 four-cell preembryos transferred in SET cycles (n = 861) were analyzed retrospectively for outcome in terms of implantation rate and its relation to the number of visible mononucleate blastomeres (MNBs), the degree of fragmentation, and equality of blastomeres.


METHODS
Light microscopic evaluation of preembryos before transfer on day 2.


METHODS
Implantation rate.


RESULTS
The number of MNBs was found to be related to implantation, whereas blastomere equality and rate of embryo fragmentation were not. The implantation rate was statistically significantly higher in cycles where a four-cell preembryo with four MNBs was transferred than after transfer of a four-cell preembryo with zero to three MNBs (42% versus 22%). In a logistic regression analysis, nucleation of all blastomeres was the only morphologic parameter that was associated with the implantation rate.


CONCLUSIONS
Evaluation of nuclear status of four-cell preembryos is important in predicting implantation potential. Visualization of four MNBs in a four-cell preembryo predicted a statistically significant higher implantation rate than in cases where not all four blastomeres were mononucleate.
Saldeen
Pia
P
IVF Clinic CURA, Malmö, Sweden. pia.saldeen@curakliniken.se
Sundström
Per
P
D000328:Adult; D001755:Blastocyst / Q000166:cytology* / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology*; D010064:Embryo Implantation / Q000502:physiology*; D004624:Embryo Transfer / Q000706:statistics & numerical data; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D016896:Treatment Outcome