Promoter activation of pepper class II basic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2-overexpressing Arabidopsis.
The activation of the CAChi2 promoter as the result of bacterial infection and osmotic stresses was examined using the Agrobacterium-mediated transient expression assay. Several stress-related cis-acting elements were revealed within the upstream genomic sequence of the CAChi2 gene. In tobacco leaf tissues transiently transformed with the CAChi2 promoter-beta-glucuronidase (GUS) gene, the CAChi2 promoter was up-regulated by Pseudomonas syringae pv. tabaci infection. The CAChi2-GUS activation was closely related to osmotic stresses, including treatment with mannitol and NaCl. The -378 CAChi2 promoter was sufficient for the CAChi2 gene induction by salicylic acid treatment. CAChi2 overexpression in the transgenic Arabidopsis plants enhanced bacterial disease resistance against Pseudomonas syringae pv. tomato infection. CAChi2-overexpressing Arabidopsis plants also exhibited increased tolerance to NaCl-induced osmotic stresses during seed germination and seedling growth. CAChi2 overexpression induced the expression of the NaCl stress-responsive gene RD29A in the absence of NaCl stress. The CAChi2-overexpressing transgenic plants exhibited increased sensitivity to abscisic acid during seed germination.
Hong
Jeum Kyu
JK
Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University, Anam-dong, Seoul 136-713, Korea. bkhwang@korea.ac.kr.
Hwang
Byung Kook
BK
D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D002212:Capsicum / Q000235:genetics* / Q000378:metabolism; D002688:Chitinases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000378:metabolism / Q000382:microbiology; D011401:Promoter Regions, Genetic; D044224:Pseudomonas syringae; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012231:Rhizobium / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism; D014157:Transcription Factors / Q000502:physiology; D015533:Transcriptional Activation
D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D012965:Sodium Chloride; D000040:Abscisic Acid; D002688:Chitinases; D020156:Salicylic Acid