Expression of a receptor kinase in Arabidopsis roots is stimulated by the basidiomycete Piriformospora indica and the protein accumulates in Triton X-100 insoluble plasma membrane microdomains.
Piriformospora indica, an endophytic fungus of the Sebacinaceae family, colonises the roots of a wide variety of plant species and promotes their growth, in a manner similar to mycorrhizal fungi. We demonstrate that the fungus also interacts with the non-mycorrhizal host Arabidopsis thaliana. Promotion of root growth was detectable even before noticeable root colonization, and was accompanied by a massive transfer of phosphate from the media to the aerial parts of the seedlings. During the recognition period of both organisms, the message for a receptor kinase with leucine-rich repeats is transiently upregulated. The kinase is located in Triton X-100-insoluble plasma membrane microdomains. Thus, this is one of the earliest events of a plant root in response to a fungus reported to date.
Shahollari
Bationa
B
Institute for General Botany and Plant Physiology, Friedrich-Schiller-University Jena, Dornburger Str 159, D-07743 Jena, Germany.
Varma
Ajit
A
Oelmüller
Ralf
R
D017360:Arabidopsis / Q000201:enzymology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000096:biosynthesis; D001487:Basidiomycota / Q000502:physiology*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D021962:Membrane Microdomains / Q000201:enzymology; D038821:Mycorrhizae; D010770:Phosphotransferases / Q000096:biosynthesis*; D018517:Plant Roots / Q000201:enzymology* / Q000382:microbiology*; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D013997:Time Factors
D029681:Arabidopsis Proteins; D011956:Receptors, Cell Surface; D010770:Phosphotransferases