LIS1 RNA interference blocks neural stem cell division, morphogenesis, and motility at multiple stages.
Mutations in the human LIS1 gene cause the smooth brain disease classical lissencephaly. To understand the underlying mechanisms, we conducted in situ live cell imaging analysis of LIS1 function throughout the entire radial migration pathway. In utero electroporation of LIS1 small interference RNA and short hairpin dominant negative LIS1 and dynactin cDNAs caused a dramatic accumulation of multipolar progenitor cells within the subventricular zone of embryonic rat brains. This effect resulted from a complete failure in progression from the multipolar to the migratory bipolar state, as revealed by time-lapse analysis of brain slices. Surprisingly, interkinetic nuclear oscillations in the radial glial progenitors were also abolished, as were cell divisions at the ventricular surface. Those few bipolar cells that reached the intermediate zone also exhibited a complete block in somal translocation, although, remarkably, process extension persisted. Finally, axonal growth also ceased. These results identify multiple distinct and novel roles for LIS1 in nucleokinesis and process dynamics and suggest that nuclear position controls neural progenitor cell division.
Tsai
Jin-Wu
JW
Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
Chen
Yu
Y
Kriegstein
Arnold R
AR
Vallee
Richard B
RB
D000818:Animals; D001369:Axons / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D019556:COS Cells; D002455:Cell Division / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D018076:DNA, Complementary; D000072159:Dynactin Complex; D018274:Electroporation; D005260:Female; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D018715:Microscopy, Video; D008869:Microtubule-Associated Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009024:Morphogenesis / Q000235:genetics*; D009154:Mutation; D019579:Neocortex / Q000166:cytology; D011247:Pregnancy; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000196:embryology; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D015415:Biomarkers; D018076:DNA, Complementary; D000072159:Dynactin Complex; D035683:MicroRNAs; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins