title: [Experimental study on effect of jinye baidu preparation in inhibiting human cytomegalovirus protein kinase pul 97].
abstract: OBJECTIVE
  To study the inhibitory effect of Jinye Baidu Preparation (JBP), a Chinese medicinal preparation, on human cytomegalovirus protein kinase pu197 and to explore its molecular mechanism in treating human cytomegalovirus (HCMV) infection.
  
  
  METHODS
  Expression of the HCMV pu197mRNA in infected cells was measured by semi-quantitative RT-PCR before and after intervention of JBP or Ganciclovir (GCV), and effect of the two medicines on the proliferation activity of the infected cells was observed by MTT.
  
  
  RESULTS
  Both JBP and GCV showed obvious inhibitory action on HCMV pu197mRNA. They could significantly enhance the proliferation activity of the cells 72 hours after HCMV infection.
  
  
  CONCLUSIONS
  JBP could inhibit the gene expression and duplication of HCMV by inhibiting the gene expression of HCMV protein kinase pu197 to enhance the proliferation activity of the infected cells so as to achieve its anti-virus action.
authors.affiliation: Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan. yuanhui02@yeah.net
authors.forename: Hui
authors.initials: H
authors.lastname: Yuan
authors.forename: Liang-Zhen
authors.initials: LZ
authors.lastname: Wen
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Jixiao
authors.initials: J
authors.lastname: Liu
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D003587:Cytomegalovirus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology / Q000821:virology; D006801:Humans; D008168:Lung / Q000166:cytology; D011494:Protein Kinases / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D014764:Viral Proteins; D011494:Protein Kinases