[Effects of overexpression of PEMT2 on expression and translocation of different PKC isoforms in rat hepatoma cells].
OBJECTIVE
To explore the mechanism of cell proliferation inhibition by transfecting phosphatidylethanolamine N-methyltransferase 2 gene (PEMT2).


METHODS
The expression and translocation of different isoforms of protein kinase C (PKC) in cells were observed with immunocytochemistry and Western blot techniques. The content of diacylglycerol (DAG) was analyzed with high performance thin layer chromatography (HPTLC) technique.


RESULTS
Transfection of PEMT2 can inhibit the expression of cPKC alpha, but obviously promotes the expression and translocation from cytosol to plasma membrane of cPKC beta2. At the same time, the content of DAG was decreased in the transfected cells. Expression and translocation of other PKC isoforms were not changed by PEMT2 transfection.


CONCLUSIONS
Effects of overexpression of PEMT2 on the expression and translocation of different PKC isoforms might be related to the mechanism of cell proliferation inhibition and apoptosis induced by transfecting PEMT2.
Li
Ya-li
YL
Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116027, China.
Ma
Ke-li
KL
Zou
Wei
W
Xia
Quan
Q
Cui
Zhao-chun
ZC
D000818:Animals; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000473:pathology; D050918:Phosphatidylethanolamine N-Methyltransferase / Q000096:biosynthesis* / Q000235:genetics; D020033:Protein Isoforms; D011493:Protein Kinase C / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D014162:Transfection
D020033:Protein Isoforms; C494489:PEMT protein, human; D050918:Phosphatidylethanolamine N-Methyltransferase; D011493:Protein Kinase C