Glycine metabolism in intact leaves by in vivo 13C and 15N labeling.
Solid-state (13)C NMR measurements of intact soybean leaves labeled by (13)CO(2) (at subambient concentrations) show that excess glycine from the photorespiratory C(2) cycle (i.e. glycine not part of the production of glycerate in support of photosynthesis) is either fully decarboxylated or inserted as (13)C-labeled glycyl residues in proteins. This (13)C incorporation in leaf protein, which is uniformly (15)N labeled by (15)NH(4)(15)NO(3), occurs as soon as 2 min after the start of (13)CO(2) labeling. In those leaves with lower levels of available nitrogen (as measured by leaf nitrate and glutamine-glutamate concentrations), the excess glycine is used primarily as glycyl residues in protein.
Cegelski
Lynette
L
Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.
Schaefer
Jacob
J
D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D005998:Glycine / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism; D009587:Nitrogen Isotopes; D010788:Photosynthesis; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D013025:Glycine max / Q000378:metabolism*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D010940:Plant Proteins; D002245:Carbon Dioxide; D009584:Nitrogen; D005998:Glycine