title: A tightly regulated molecular motor based upon T7 RNA polymerase.
abstract: Controlled movement of materials or molecules within the nanometer range is essential in many applications of nanotechnology. Here we report the capture, movement, and release of cargo molecules along DNA by a modified form of T7 RNA polymerase (RNAP) in a manner that is controlled by the sequence of the DNA. Using single-molecule methods, we visualize the assembly and manipulation of nanodevices and the ability to harness rotary and linear forces of the RNAP motor.
authors.affiliation: Department of Microbiology and Immunology and Graduate Program in Molecular and Cellular Biology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203-2098, USA.
authors.forename: Richard T
authors.initials: RT
authors.lastname: Pomerantz
authors.forename: Ravi
authors.initials: R
authors.lastname: Ramjit
authors.forename: Zoher
authors.initials: Z
authors.lastname: Gueroui
authors.forename: Christophe
authors.initials: C
authors.lastname: Place
authors.forename: Michael
authors.initials: M
authors.lastname: Anikin
authors.forename: Sanford
authors.initials: S
authors.lastname: Leuba
authors.forename: Jordanka
authors.initials: J
authors.lastname: Zlatanova
authors.forename: William T
authors.initials: WT
authors.lastname: McAllister
mesh_terms: D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism; D004800:Enzymes, Immobilized; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology; D012313:RNA / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D020409:Molecular Motor Proteins; D014764:Viral Proteins; D012313:RNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases