title: Induction of branchial ion transporter mRNA expression during acclimation to salinity change in the euryhaline crab Chasmagnathus granulatus.
abstract: Using quantitative real-time PCR, the expression of mRNAs encoding three transport-related proteins and one putative housekeeping protein was analyzed in anterior and posterior gills of the euryhaline crab Chasmagnathus granulatus following transfer from isosmotic conditions (30 per thousand salinity) to either dilute (2 per thousand) or concentrated (45 per thousand) seawater. Modest changes were observed in the abundance of mRNAs encoding the housekeeping protein arginine kinase and the vacuolar-type H(+)-ATPase B-subunit, both of which were highly expressed under all conditions. By contrast, the expression of Na(+)/K(+)-ATPase alpha-subunit mRNA and Na(+)/K(+)/2Cl(-) cotransporter mRNA was strongly responsive to external salinity. During acclimation to dilute seawater, cotransporter mRNA increased 10-20-fold in posterior gills within the first 24 h while Na(+)/K(+)-ATPase alpha-subunit mRNA increased 35-55-fold. During acclimation to concentrated seawater, cotransporter mRNA increased 60-fold by 96 h and Na(+)/K(+)-ATPase alpha-subunit increased approximately 25-fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.
authors.affiliation: Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Ciudad Universitaria, Argentina.
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Luquet
authors.forename: Dirk
authors.initials: D
authors.lastname: Weihrauch
authors.forename: Mihaela
authors.initials: M
authors.lastname: Senek
authors.forename: David W
authors.initials: DW
authors.lastname: Towle
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D000704:Analysis of Variance; D000818:Animals; D003386:Brachyura / Q000378:metabolism*; D017931:DNA Primers; D005786:Gene Expression Regulation; D005880:Gills / Q000378:metabolism; D016623:Ion Pumps / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D012623:Seawater / Q000737:chemistry*; D013997:Time Factors
chemical_list: D017931:DNA Primers; D016623:Ion Pumps; D012333:RNA, Messenger