title: Characterization of avian paramyxovirus type 1 strains isolated in Germany during 1992 to 1996.
abstract: In Germany all avian paramyxoviruses (APMV) isolated in regional laboratories are collected and characterized by the National Reference Laboratory. From 1992 until 1996, 635 APMV-1 virus isolates were submitted from almost all regions. Of these viruses, 371 were isolated from chickens, 39 from other poultry, 171 from pigeons and 54 from exotic birds. All isolates were examined for virulence in intracerebral pathogenicity index (ICPI) tests, for their ability to react with a panel of monoclonal antibodies (mAb) and their thermostability. In addition, the nucleotide sequences of the cleavage site of the fusion protein of a few virus isolates were determined. Most isolates from chickens and other poultry were of the velogenic pathotype. This virus was responsible for the epizootic in 1993 to 1995 in many small flocks. The same virus was obtained from some pigeons and some exotic birds. The pathogenicity of the velogenic/epizootic virus was high with most viruses giving ICPI values of 1.8 to 1.9, and the sequences of the cleavage site of all velogenic isolates tested were closely related. However, viruses isolated at the beginning of the epizootic period differed from viruses isolated towards the end in their reaction with some mAbs. 149 virus isolates were identified as pigeon variant PMV-1 (PPMV-1). Most of these were obtained from pigeons but a few were isolated from chickens and other birds. Most lentogenic isolates proved to be vaccine virus strains.
authors.affiliation: Institute for Applied Virology, Friedrich-Loeffler-Institute, Federal Research Centre for Virus Diseases of Animals, Insel Riems, Germany. ortrud.weiner@rie.bfav.de
authors.forename: O
authors.initials: O
authors.lastname: Werner
authors.forename: A
authors.initials: A
authors.lastname: Römer-Oberdörfer
authors.forename: B
authors.initials: B
authors.lastname: Köllner
authors.forename: R J
authors.initials: RJ
authors.lastname: Manvell
authors.forename: D J
authors.initials: DJ
authors.lastname: Alexander