title: Comparative evaluation of TRI-DOT Rapid HIV test with fourth-generation ELISA for the detection of human immunodeficiency virus.
abstract: This study evaluated the TRI-DOT Rapid HIV test for the early detection of human immunodeficiency virus (HIV) infection in comparison with a fourth-generation ELISA (Vironostika HIV Uniform II). Of 23,609 sera, seven (0.03%) gave discordant results. Six of these were reactive only by the fourth-generation assay and were p24 antigen-positive by VIDAS DUO, Western blot and qualitative RT-PCR tests. The remaining discordant serum was considered to be false-positive by the TRI-DOT assay, as it was negative by repeat ELISA and Western blot tests. The sensitivity and specificity of the TRI-DOT test were 99.48% and 99.99%, respectively, compared with the fourth-generation ELISA.
authors.affiliation: Department of Microbiology, Nizam's Institute of Medical Sciences, Hyderabad, AP, India.
authors.forename: T
authors.initials: T
authors.lastname: Sudha
authors.forename: V D
authors.initials: VD
authors.lastname: Teja
authors.forename: M
authors.initials: M
authors.lastname: Gopal
authors.forename: M
authors.initials: M
authors.lastname: Rajesh
authors.forename: V
authors.initials: V
authors.lastname: Lakshmi
mesh_terms: D015492:AIDS Serodiagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000097:blood; D015488:HIV Antigens / Q000097:blood*; D016655:HIV Core Protein p24 / Q000097:blood; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification*; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity
chemical_list: D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24