Improved system for construction and analysis of single-copy beta-galactosidase operon fusions in Yersinia enterocolitica.
We report a significantly improved system for studying single-copy lacZ operon fusions in Yersinia enterocolitica: a simple procedure for the stable integration of lacZ operon fusions into the ara locus and a strain with a deletion mutation that abolishes the low level of endogenous beta-galactosidase activity.
Maxson
Michelle E
ME
Department of Microbiology MSB 228, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.
Darwin
Andrew J
AJ
D017353:Gene Deletion; D018628:Gene Dosage; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors; D007763:Lac Operon / Q000235:genetics*; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D015008:Yersinia enterocolitica / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase