[Biostability and calcification resistance of valved bovine jugular veins stabilized by dye-mediated photooxidation].
This study was conducted to make an in vitro evaluation of the biological characteristic of valved bovine jugular veins stabilized by dye-mediated photooxidation. The fresh valved bovine jugular veins were stabilized by dye-mediated photooxidation. The glutaraldehyde-treated veins and fresh veins served as controls. The samples were subjected to protein extraction assay and pepsin digestion assay, and were analyzed by polyacrylamide gel electrophoresis. The rat subcutaneous model for assessment of tissue calcification and immunologic properties was used. Calcium levels were determined by elemental analysis on dried samples and von Kossa staining. Both photooxidized and glutaraldehyde-treated tissues were resistant to protein extraction and pepsin digestion, compared to fresh tissue. Calcification levels were much lower for photooxidized tissues when compared with those for both glutaraldehyde-treated and fresh tissues. The perigraft infiltrate was lower in the photooxidized tissues than in the fresh and glutaraldehyde-treated tissues. Valved bovine jugular veins stabilized by dye-mediated photooxidation were biostable and resistant to calcification, and the immunologic property was well.
Li
Shengli
S
Department of Surgery, Fuwoai Heart Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China. lichamp@yahoo.com
Hu
Shengshou
S
Zhou
Jianye
J
D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D002114:Calcinosis / Q000473:pathology / Q000517:prevention & control; D002118:Calcium / Q000032:analysis*; D002417:Cattle; D004396:Coloring Agents / Q000737:chemistry; D006801:Humans; D007601:Jugular Veins / Q000473:pathology / Q000502:physiology*; D008422:Materials Testing; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010777:Photochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D016707:Tissue Fixation / Q000379:methods
D004396:Coloring Agents; D010083:Oxidants, Photochemical; D002118:Calcium