Cytokinesis depends on the motor domains of myosin-II in fission yeast but not in budding yeast.
Budding yeast possesses one myosin-II, Myo1p, whereas fission yeast has two, Myo2p and Myp2p, all of which contribute to cytokinesis. We find that chimeras consisting of Myo2p or Myp2p motor domains fused to the tail of Myo1p are fully functional in supporting budding yeast cytokinesis. Remarkably, the tail alone of budding yeast Myo1p localizes to the contractile ring, supporting both its constriction and cytokinesis. In contrast, fission yeast Myo2p and Myp2p require both the catalytic head domain as well as tail domains for function, with the tails providing distinct functions (Bezanilla and Pollard, 2000). Myo1p is the first example of a myosin whose cellular function does not require a catalytic motor domain revealing a novel mechanism of action for budding yeast myosin-II independent of actin binding and ATPase activity.
Lord
Matthew
M
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520-8103, USA.
Laves
Ellen
E
Pollard
Thomas D
TD
D000199:Actins / Q000502:physiology; D000818:Animals; D048749:Cytokinesis / Q000235:genetics*; D018995:Myosin Heavy Chains / Q000235:genetics / Q000502:physiology*; D024462:Myosin Type II / Q000235:genetics / Q000502:physiology*; D024701:Myosin Type V / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D004718:Saccharomycetales / Q000235:genetics / Q000502:physiology*; D012568:Schizosaccharomyces / Q000235:genetics / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000502:physiology*
D000199:Actins; C112936:MYO1 protein, S cerevisiae; C077512:MYO2 protein, S cerevisiae; C110514:Myp2 protein, S pombe; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D024462:Myosin Type II; D024701:Myosin Type V; D018995:Myosin Heavy Chains