Demonstration of P2Y4 purinergic receptors in the HT-29 human colon cancer cell line.
1 The aim of the current study was to investigate the existence of P 2 Y(4) purinergic receptors in the HT-29 human colon cancer cell line. 2 We utilized Western blots and immunocytochemistry for the analysis. 3 Western blotting demonstrated two bands that could not be found after the antibody had been preabsorbed with the control peptide, suggesting that both bands are related to the P 2 Y(4) purinergic receptor. 4 Immunocytochemistry showed immunoreactivity for the P 2 Y(4) purinergic receptor localized in the cytoplasm of the HT-29 cells. 5 This is the first demonstration of the protein expression of P 2 Y(4) purinergic receptors in a human colon cancer cell line.
Delbro
D S
DS
Department of Surgery, Institute of Surgical Sciences, Sahlgrenska University Hospital, SE-413 45 Göteborg, Sweden.
Nylund
G
G
Nordgren
S
S
D015153:Blotting, Western; D003593:Cytoplasm / Q000378:metabolism; D019073:HT29 Cells / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D018048:Receptors, Purinergic P2 / Q000032:analysis*
D018048:Receptors, Purinergic P2; C104749:purinoceptor P2Y4