Influence of diamino acid derivatives of protoporphyrin IX on mouse immunological system: preliminary results.
Immunological response related to photodynamic therapy (PDT) is one of the basic elements that influence on the efficiency of this cancer treatment method. Diamino acid derivatives of protoporphyrin IX are promising photosensitizing agents that are intended to be the components of new anti-tumor drug. The influence of three derivatives - PP(Ser)(2)Arg(2), PP(Ala)(2)Arg(2), PP(Phe)(2)Arg(2) and a mixture of these compounds called Sensyphyrine on mouse immunological system was investigated where animals were exposed and unexposed to the laser irradiation. Porphyrins solutions were injected intravenously, mice were irradiated with the red diode laser at lambda=632 nm. Cells and blood samples were taken at time intervals after irradiation. The levels of interleukin-6, interleukin-1beta and the production of reactive forms of nitrogen by macrophages were determined. The results show that all of the diamino acid derivatives of protoporphyrin IX indicate an immunological response when the light is applied. Each of the porphyrin revealed different impact on mice immunological system. The most potent stimulant properties disclosed PP(Phe)(2)Arg(2) derivative for which the highest values of IL-1beta, IL-6 and NO(2)(-) were noticed. The weakest immunological activation revealed PP(Ser)(2)Arg(2) derivative.
Kwitniewski
Mateusz
M
Department of Molecular Microbiology and Serology, National Salmonella Centre, Medical University of Gdansk, 9b Powstania Styczniowego Street, PL-81519 Gdynia, Poland.
Kunikowska
Danuta
D
Dera-Tomaszewska
Bozena
B
Tokarska-Pietrzak
Ewa
E
Dziadziuszko
Halina
H
Graczyk
Alfreda
A
Glosnicka
Renata
R
D000599:Amino Acids, Diamino / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D051379:Mice / Q000276:immunology* / Q000378:metabolism; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011524:Protoporphyrins / Q000494:pharmacology*; D044967:Serum / Q000378:metabolism; D013997:Time Factors
D000599:Amino Acids, Diamino; D015850:Interleukin-6; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D009569:Nitric Oxide; C028025:protoporphyrin IX