Assay of CCNU in tumor by RP-HPLC.
This paper reports a RP-HPLC method for the determination of Lomustine (CCNU) in mouse tumor tissue. CCNU was separated and determined on a reverse-phase C18 5 microm column with a mobile phase of H2O-acetonitrile (54:46), detected at 254 nm and no internal standard. The calibration curve was linear (r = 0.9999) within the range of 0.98-15.68 microg/ml for CCNU. The recovery ratio of CCNU was 68.64% (n = 5). The relative standard deviation (RSD) was 0.32%. This method is simple and accurate.
Zhao
Zhiquan
Z
National Pharmaceutical Research Center, Lunan Pharmaceutical Corporation, Shandong, China.
Sun
Yong
Y
Zhang
Qiqing
Q
Jiang
Jun
J
D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008130:Lomustine / Q000008:administration & dosage / Q000032:analysis*; D008297:Male; D051379:Mice; D012509:Sarcoma / Q000737:chemistry* / Q000188:drug therapy
D018906:Antineoplastic Agents, Alkylating; D008130:Lomustine