title: Activation of immature microglia in response to stab wound in embryonic quail retina.
abstract: Activation of mature (ramified) microglia in response to injury in the adult central nervous system (CNS) is well documented. However, the response of immature (ameboid) microglia to injury in the developing CNS has received little attention. In this study, a stab wound was made in embryonic quail retinas at incubation days 7 and 9, and the response of retinal microglial cells was analyzed at different times between days 1 and 37 postinjury. The appearance of microglial cells within the wound occurred at the same time as the arrival of the first migrating ameboid microglial cells at an equivalent area in control retinas. Therefore, no specific attraction of microglia toward the wound was observed. Microglial cells in the wound had phenotypic features similar to those of activated microglia in the adult CNS. Thus, their shape was more compact compared with microglial cells outside the wound, expression of the molecule recognized by the QH1 antibody was up-regulated, and their lysosomal compartment was markedly increased. Transitional forms between normal ameboid and activated-like microglial cells were seen at the wound edge, supporting the view that ameboid microglia become activated when they contact the wound during the normal course of their migration in the retina. The microglial reaction was maintained within the wound at 37 days postinjury. In addition to the stab wound, secondary damage areas were found in experimental retinas. Activated cells could still be observed in these areas at 37 days postinjury.
authors.affiliation: Departamento de Biología Celular, Facultad de Ciencias, Universidad de Granada, and Banco de Líneas Celulares de Andalucía, Hospital Universitario San Cecilio, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Sánchez-López
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Cuadros
authors.forename: Ruth
authors.initials: R
authors.lastname: Calvente
authors.forename: Mohamed
authors.initials: M
authors.lastname: Tassi
authors.forename: José L
authors.initials: JL
authors.lastname: Marín-Teva
authors.forename: Julio
authors.initials: J
authors.lastname: Navascués
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D048430:Cell Shape / Q000502:physiology; D003370:Coturnix / Q000196:embryology*; D004625:Embryo, Nonmammalian; D017628:Microglia / Q000166:cytology* / Q000473:pathology / Q000502:physiology*; D012160:Retina / Q000166:cytology* / Q000196:embryology* / Q000293:injuries; D014945:Wound Healing / Q000502:physiology*; D014951:Wounds, Stab / Q000473:pathology / Q000503:physiopathology