Direct application of the INNO-LiPA Rif.TB line-probe assay for rapid identification of Mycobacterium tuberculosis complex strains and detection of rifampin resistance in 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis.
The INNO-LiPA Rif.TB assay for the identification of Mycobacterium tuberculosis complex strains and the detection of rifampin (RIF) resistance has been evaluated with 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis (MDR-TB). The sensitivity when compared to conventional identification/culture methods was 82.2%, and the specificity was 66.7%; the sensitivity and specificity were 100.0% and 96.9%, respectively, for the detection of RIF resistance. This assay has the potential to provide rapid information that is essential for the effective management of MDR-TB.
Viveiros
Miguel
M
Unidade de Micobacterias do Instituto de Higiene e Medicina Tropical, Lisboa, Portugal.
Leandro
Clara
C
Rodrigues
Liliana
L
Almeida
Josefina
J
Bettencourt
Rosário
R
Couto
Isabel
I
Carrilho
Lurdes
L
Diogo
José
J
Fonseca
Ana
A
Lito
Luís
L
Lopes
João
J
Pacheco
Teresa
T
Pessanha
Mariana
M
Quirim
Judite
J
Sancho
Luísa
L
Salfinger
Max
M
Amaral
Leonard
L
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D012321:DNA-Directed RNA Polymerases; D024881:Drug Resistance, Bacterial; D006801:Humans; D015994:Incidence; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012293:Rifampin / Q000494:pharmacology*; D013183:Sputum / Q000382:microbiology*; D013997:Time Factors; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000382:microbiology
D000904:Antibiotics, Antitubercular; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin