Effects of caffeic acid phenethyl ester on endotoxin-induced uveitis in rats.
OBJECTIVE
Caffeic acid phenethyl ester (CAPE) has antimicrobial, anti-inflammatory, antioxidant, immunomodulatory, and carcinostatic properties. In this study, the efficacy of CAPE in endotoxin-induced uveitis (EIU) in rats is investigated.


METHODS
EIU was induced by a footpad injection of lipopolysaccharide (LPS). In the treatment group, 10 micromol/kg CAPE was injected intraperitoneally immediately after LPS injection. At 24 hr after LPS injection, the number of infiltrating cells, protein concentration, and levels of myeloperoxidase (MPO) in aqueous humor; malondialdehyde (MDA), MPO, and total antioxidant levels in serum were determined. Eyes were enucleated for histopathologic evaluation, and, counting inflammatory cells in iris-ciliary body (ICB), the efficacy of treatment was determined.


RESULTS
CAPE significantly suppressed LPS-induced increase in the number of inflammatory cells (p = 0.0001), protein concentration (p = 0.0001), and MPO levels (p = 0.0001) in aqueous humor as well as MDA (p = 0.001) and MPO (p = 0.0001) levels in serum. Histopathologic evaluation of ICB showed significant reduction in the inflammatory cell counts in the treatment group (p = 0.0001).


CONCLUSIONS
CAPE was found efficient in suppressing inflammation and ocular tissue damage induced by LPS in rats.
Yilmaz
Ayça
A
Mersin University School of Medicine, Ophthalmology Department, Mersin, Turkey. aycayilmaz@mersin.edu.tr
Yildirim
Ozlem
O
Tamer
Lülüfer
L
Oz
Ozay
O
Cinel
Leyla
L
Vatansever
Halil
H
Değirmenci
Ulaş
U
Kanik
Arzu
A
Atik
Uğur
U
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D001082:Aqueous Humor / Q000166:cytology / Q000378:metabolism; D002109:Caffeic Acids / Q000627:therapeutic use*; D002924:Ciliary Body / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007498:Iris / Q000378:metabolism / Q000473:pathology; D008070:Lipopolysaccharides; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium; D014605:Uveitis / Q000139:chemically induced / Q000188:drug therapy*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002109:Caffeic Acids; D005136:Eye Proteins; D008070:Lipopolysaccharides; D009195:Peroxidase; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol