title: Angiogenesis: the major abnormality of the keratin-14 IL-4 transgenic mouse model of atopic dermatitis.
abstract: OBJECTIVE
  Angiogenesis plays an important role in psoriasis, but its role in atopic dermatitis is unknown. The authors examined the dermal microvasculature of an IL-4 transgenic mouse model of atopic dermatitis to determine whether angiogenesis was present.
  
  
  METHODS
  Transmission and scanning electron microscopy and confocal microscopy studies were performed.
  
  
  RESULTS
  Transmission electron microscopy showed sprouting, transcapillary pillars of intussusception, thickened endothelial cells with large nuclei, and increased interendothelial junctional cleft number and length. Compared to nontransgenic littermates, there was a significant increase in the lengths and numbers of the interendothelial junctional clefts, along with a decrease in the length ratios of tight junction to interendothelial junctional clefts in both the early and late disease stages. In the early and late skin lesions, scanning electron microscopy of vascular corrosion casts showed disorganization of the capillary network hierarchy with increased density of capillary sprouts. Confocal microscopy of the animals with early and late skin lesions showed significant reduction in tight junction protein claudin-5.
  
  
  CONCLUSIONS
  Angiogenesis is the major pathologic feature in this model of atopic dermatitis. The chronic skin inflammation is intertwined with and may cause the angiogenesis, but the angiogenesis itself is likely to be important in this disease process.
authors.affiliation: Department of Dermatology, University of Illinois, Chicago, 60612, USA.
authors.forename: Rania
authors.initials: R
authors.lastname: Agha-Majzoub
authors.forename: Robert P
authors.initials: RP
authors.lastname: Becker
authors.forename: Dean E
authors.initials: DE
authors.lastname: Schraufnagel
authors.forename: Lawrence S
authors.initials: LS
authors.lastname: Chan
mesh_terms: D000818:Animals; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D020405:Dermis / Q000098:blood supply* / Q000648:ultrastructure; D004195:Disease Models, Animal; D015847:Interleukin-4 / Q000235:genetics* / Q000378:metabolism; D053547:Keratin-14; D007633:Keratins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: D053547:Keratin-14; C507446:Krt14 protein, mouse; D015847:Interleukin-4; D007633:Keratins