LC-MS/MS-analysis of prostaglandin E2 and D2 in microdialysis samples of rats.
For the determination of prostaglandins in microdialysis samples, usually immunoassays are used. However, these assays may show cross-reactivity among various prostaglandins. To overcome this problem a specific method for the determination of prostaglandin E2 and D2 in rat microdialysis samples by using liquid chromatography-electrospay ionization-tandem mass spectrometry (LC-ESI-MS/MS) is described. Prostaglandin E2 and D2 were extracted from microdialysis samples with liquid-liquid extraction using deuterated prostaglandin D2, [2H4]-PGD2, as internal standard. Subsequently, prostaglandins were separated with a phenomenex Synergi Hydro-RP column and determined with a PE Sciex API 3000 mass spectrometer equipped with a turbo ion spray interface operating in negative ionization mode. The method showed a LLOQ of 25 pg/ml for prostaglandin E2 and 50 pg/ml for prostaglandin D2. The applicability of the method is shown in rat spinal cord microdialysis samples following peripheral nociceptive stimulation.
Schmidt
Ronald
R
pharmazentrum frankfurt/ZAFES/Institut für Klinische Pharmakologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt, Theodor Stern Kai 7, Haus 75/4.OG, 60590 Frankfurt, Germany. ronald.schmidt@em.uni-frankfurt.de
Coste
Ovidiu
O
Geisslinger
Gerd
G
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D015232:Dinoprostone / Q000032:analysis*; D004355:Drug Stability; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D017551:Microdialysis / Q000379:methods*; D015230:Prostaglandin D2 / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013116:Spinal Cord / Q000737:chemistry
D015232:Dinoprostone; D015230:Prostaglandin D2