Immunohistochemical localisation of PDE5 in Leydig and myoid cells of prepuberal and adult rat testis.
Expression of phosphodiesterase 5 (PDE5) in the rat testis at several pre and postnatal developmental stages was investigated by immunohistochemistry. The enzyme was localised in vascular smooth muscle cells, as well as in Leydig and peritubular cells. The latter were identified as myoid, based on their immunoreactivity to desmin and alpha-smooth muscle actin. The presence of PDE5 in myoid cells was confirmed by Western blot analysis and immunohistochemistry performed on highly purified cell fractions, obtained from 16-day-old rats. The expression of PDE5 in these somatic cells of rat testis is discussed in view of the roles played by cGMP signal transduction pathways in the mammalian male reproductive function.
Scipioni
Angela
A
Department of Cellular and Developmental Biology, University La Sapienza, Piazzale Aldo Moro 5, Rome, 00185, Italy.
Stefanini
Stefania
S
Santone
Rocco
R
Giorgi
Mauro
M
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002458:Cell Fractionation; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; D047109:Fetal Development / Q000502:physiology*; D019084:Fluorescent Antibody Technique, Indirect; D005865:Gestational Age; D007124:Immunoenzyme Techniques; D007985:Leydig Cells / Q000166:cytology / Q000201:enzymology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000166:cytology / Q000378:metabolism; D013737:Testis / Q000196:embryology* / Q000201:enzymology
D000199:Actins; D015415:Biomarkers; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; C517384:Pde5a protein, rat