Rapid and quantitative detection of hepatitis A virus from green onion and strawberry rinses by use of real-time reverse transcription-PCR.
In this study, an immunomagnetic capture method and a real-time reverse transcription-PCR assay were used to quantify hepatitis A virus (HAV) in green onion and strawberry rinses. This combined protocol detected as low as 0.5 PFU HAV in produce rinses and concentrated HAV levels up to 20-fold.
Shan
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XC
Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada. mgriffit@uoguelph.ca.
Wolffs
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Griffiths
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MW
D002021:Buffers; D031985:Fragaria / Q000821:virology*; D030041:Hepatitis A virus / Q000235:genetics / Q000302:isolation & purification*; D018189:Immunomagnetic Separation; D019697:Onions / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
D002021:Buffers; D012367:RNA, Viral