Comparative effects of palm vitamin E and alpha-tocopherol on healing and wound tissue antioxidant enzyme levels in diabetic rats.
The effect of supplementing 200 mg/kg body weight palm vitamin E (PVE) and 200 mg/kg body weight alpha-tocopherol (alpha-Toc) on the healing of wounds in streptozotocin-induced diabetic rats was evaluated. The antioxidant potencies of these two preparations of vitamin E were also evaluated by determining the antioxidant enzyme activities, namely, glutathione peroxidase (GPx) and superoxide dismutase (SOD), and malondialdehyde (MDA) levels in the healing of dermal wounds. Healing was evaluated by measuring wound contractions and protein contents in the healing wounds. Cellular redistribution and collagen deposition were assessed morphologically using cross-sections of paraffin-embedded day-10 wounds stained according to the Van Gieson method. GPx and SOD activities as well as MDA levels were determined in homogenates of day-10 dermal wounds. Results showed that PVE had a greater potency to enhance wound repair and induce the increase in free radical-scavenging enzyme activities than alpha-Toc. Both PVE and alpha-Toc, however, were potent antioxidants and significantly reduced the lipid peroxidation levels in the wounds as measured by the reduction in MDA levels.
Musalmah
M
M
Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. musalmah@medic.ukm.my
Nizrana
M Y
MY
Fairuz
A H
AH
NoorAini
A H
AH
Azian
A L
AL
Gapor
M T
MT
Wan Ngah
W Z
WZ
D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000473:pathology; D004798:Enzymes / Q000187:drug effects / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries / Q000473:pathology; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology; D014950:Wounds, Penetrating / Q000188:drug therapy / Q000473:pathology*; D024502:alpha-Tocopherol / Q000494:pharmacology*
D000975:Antioxidants; D004798:Enzymes; D014810:Vitamin E; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D024502:alpha-Tocopherol