title: Absolute method for the assay of oleuropein in olive oils by atmospheric pressure chemical ionization tandem mass spectrometry.
abstract: Oleuropein (OLP, 1), the active ingredient present (i) in food integrators extracted from olive leaves, (ii) in table olives, and (iii) in extra virgin olive oils is a nutraceutical whose health benefits have been widely documented. A new analytical method for its assay, which is based on the utilization of atmospheric pressure chemical ionization tandem mass spectrometry and on the use of a synthetic labeled analogue, the 4-trideuteriocarboxyoleuropein (2), as an internal standard, is presented. The results obtained with extra virgin olive oils from different cultivars and different Italian regions are discussed.
authors.affiliation: Dipartimento di Chimica, Università della Calabria, via P. Bucci, cubo 12/C, I-87030 Arcavacata di Rende (CS), Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: De Nino
authors.forename: Leonardo
authors.initials: L
authors.lastname: Di Donna
authors.forename: Fabio
authors.initials: F
authors.lastname: Mazzotti
authors.forename: Enzo
authors.initials: E
authors.lastname: Muzzalupo
authors.forename: Enzo
authors.initials: E
authors.lastname: Perri
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sindona
authors.forename: Antonio
authors.initials: A
authors.lastname: Tagarelli
mesh_terms: D001274:Atmospheric Pressure; D057888:Iridoid Glucosides; D039823:Iridoids; D000069463:Olive Oil; D010938:Plant Oils / Q000032:analysis* / Q000737:chemistry*; D011714:Pyrans / Q000032:analysis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D013055:Spectrophotometry, Infrared; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: D057888:Iridoid Glucosides; D039823:Iridoids; D000069463:Olive Oil; D010938:Plant Oils; D011714:Pyrans; C002769:oleuropein