Cytotoxicity and the levels of oxidative stress parameters in WI38 cells following 2 macrocyclic crown ethers treatment.
BACKGROUND
Crown ethers as macrocyclic polyethers possess a hydrophilic cavity surrounded by hydrophobic ring which enable them to diffuse cell membrane. We evaluated cytotoxicity effects of 15-crown-5 and 18-crown-6 and the role of oxidative stress in WI38 cells culture.


METHODS
The effect of these ethers in a range of doses (0.1 to 2 mmol/l) on the activity of antioxidant enzymes; superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), and on some macromolecules oxidative damages end products; malondialdehyde (MDA) and dityrosine were assessed by spectrometry and HPLC methods.


RESULTS
Both compounds markedly inhibited the viability of cells with respect to control particularly at doses >0.5 mmol/l after 24- or 48 h incubation. The survivals of cells were measured using MTT assay. They lowered cell's viability and significantly promoted ROS generation, increased enzyme activities and enhanced oxidative damages in which 18-crown-6 was more effective. Treating cells with 30 microm of alpha-tocopherol in addition to 2 mmol/l of crown ethers showed significant decrease on the levels of ROS, enzyme activities, MDA and dityrosine.


CONCLUSIONS
We document the oxidative radicals forming ability of the studied crown ethers and further strengthens the documentation of their cytotoxicity effects through lipid and proteins oxidation damages.
Boojar
Massod Mashhadi Akbar
MM
Department of Biology, University of Tarbiat Moalem, No: 49, Dr. Mofateh Avenue, Tehran, P.O. Box: 15614, Iran. aboojar@yahoo.com
Goodarzi
Faranak
F
D000975:Antioxidants / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D043844:Crown Ethers / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000166:cytology; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013053:Spectrophotometry; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D024502:alpha-Tocopherol / Q000494:pharmacology
D000975:Antioxidants; D043844:Crown Ethers; D017382:Reactive Oxygen Species; C042148:15-crown-5; D014443:Tyrosine; D008315:Malondialdehyde; C015762:18-crown-6; C007543:dityrosine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D024502:alpha-Tocopherol