Physiological activity of irradiated green tea polyphenol on the human skin.
Physiological activity of irradiated green tea polyphenol on the human skin was investigated for further industrial application. The green tea polyphenol was separated and irradiated at 40 kGy by y-ray. For an anti-wrinkle effect, the collagenase inhibition effect was higher in the irradiated sample (65.3%) than that of the non-irradiated control (56.8%) at 200 ppm of the concentration (p < 0.05). Collagen biosynthesis rates using a human fibroblast were 19.4% and 16.3% in the irradiated and the non-irradiated polyphenols, respectively. The tyrosinase inhibition effect, which is related to the skin-whitening effect, showed a 45.2% and 42.9% in the irradiated and the non-irradiated polyphenols, respectively, at a 100 ppm level. A higher than 90% growth inhibition on skin cancer cells (SK-MEL-2 and G361) was demonstrated in both the irradiated and the non-irradiated polyphenols. Thus, the irradiation of green tea polyphenol did not change and even increased its anti-wrinkle, skin-whitening and anticancer effects on the human skin. The results indicated that irradiated green tea polyphenol can be used as a natural ingredient with excellent physiological functions for the human skin through cosmetic or food composition.
An
Bong-Jeun
BJ
Department of Cosmeceutical Science, Daegu Haany University, Kyungsan 712-715, South Korea. anbj@dhu.ac.kr
Kwak
Jae-Hoon
JH
Son
Jun-Ho
JH
Park
Jung-Mi
JM
Lee
Jin-Young
JY
Park
Tae Soon
TS
Kim
So-Yeun
SY
Kim
Yeoung-Sun
YS
Jo
Cheorun
C
Byun
Myung-Woo
MW
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D003094:Collagen / Q000096:biosynthesis / Q000378:metabolism; D003358:Cosmetics; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D005514:Food Irradiation; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D008545:Melanoma / Q000188:drug therapy*; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D012867:Skin / Q000166:cytology; D015595:Skin Aging / Q000187:drug effects*; D012878:Skin Neoplasms / Q000188:drug therapy*; D013662:Tea
D000970:Antineoplastic Agents; D003358:Cosmetics; D005419:Flavonoids; D061965:Matrix Metalloproteinase Inhibitors; D010636:Phenols; D059808:Polyphenols; D013662:Tea; D003094:Collagen; D014442:Monophenol Monooxygenase