title: Binding of mycoplasmas to solid phase adsorbents.
abstract: The capture of mycoplasmas (M. hominis, M. buccale, M. fermentans, M. bovis, M. synoviae, M. gallisepticum and M. arthritidis) based on lipid structures and adhesion molecules present in the mycoplasmal membrane was tested using different chromatographic resins (ActiClean Etox, ClarEtox, Heparin-Actigel, Sulfated Hiflow and SulfEtox). All of the resins efficiently reduced mycoplasma concentrations in Phosphate Buffered Saline (PBS) and in Fetal Bovine Serum (FBS) by 3-8 logs in a few minutes. This technology could be used for removing mycoplasmas from tissue culture components such as serum, and for concentrating mycoplasmas in vaccine production.
authors.affiliation: Clarigen Inc, Carlsbad, CA, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Szathmáry
authors.forename: Nandani
authors.initials: N
authors.lastname: Rajapakse
authors.forename: Ibolya
authors.initials: I
authors.lastname: Székely
authors.forename: E
authors.initials: E
authors.lastname: Pitlik
authors.forename: Judit
authors.initials: J
authors.lastname: Bíró
authors.forename: Noémi
authors.initials: N
authors.lastname: Erdei
authors.forename: L
authors.initials: L
authors.lastname: Stipkovits
mesh_terms: D000327:Adsorption; D001422:Bacterial Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D002845:Chromatography; D009174:Mycoplasma / Q000302:isolation & purification* / Q000502:physiology*; D012117:Resins, Synthetic
chemical_list: D015815:Cell Adhesion Molecules; D012117:Resins, Synthetic