Method for isolation of Bacteroides bacteriophage host strains suitable for tracking sources of fecal pollution in water.
Bacteriophages infecting Bacteroides are potentially a good tool for fecal source tracking, but different Bacteroides host strains are needed for different geographic areas. A feasible method for isolating Bacteroides host strains for phages present in human fecal material is described. Useful strains were identified for application in Spain and the United Kingdom. One strain, GA-17, identified as Bacteroides thetaiotaomicron, was tested in several locations in Europe with excellent performance in Southern Europe.
Payan
Andrey
A
Department of Microbiology, University of Barcelona, Diagonal 645, 08028 Barcelona, Spain.
Ebdon
James
J
Taylor
Huw
H
Gantzer
Christophe
C
Ottoson
Jakob
J
Papageorgiou
Georgos T
GT
Blanch
Anicet R
AR
Lucena
Francisco
F
Jofre
Juan
J
Muniesa
Maite
M
D001434:Bacteriophage Typing; D001435:Bacteriophages / Q000302:isolation & purification* / Q000502:physiology; D001439:Bacteroides / Q000145:classification* / Q000302:isolation & purification / Q000821:virology; D004784:Environmental Monitoring / Q000379:methods*; D005060:Europe; D005243:Feces / Q000382:microbiology* / Q000821:virology; D006801:Humans; D013045:Species Specificity; D014876:Water Pollution