Genotyping Clostridium botulinum toxinotype A isolates from patients using amplified rDNA restriction analysis.
In this study, the application of amplified rDNA restriction analysis (ARDRA) for characterizing Clostridium botulinum toxinotype A strains isolated from individuals with botulism was evaluated. Ten restriction enzymes were tested for their suitability in ARDRA as a typing method and HhaI was selected for the best outcome. Analysis of HhaI restriction profiles of the amplified products divided C. botulinum isolates into three clusters. Non-toxigenic Clostridium sporogenes strains showed an ARDRA restriction pattern that was distinct from those observed for C. botulinum. The successful use of ARDRA for subdivision of C. botulinum in this study confirmed that this technique is a powerful method for typing of C. botulinum toxinotype A clonal diversity. In addition, it is rapid, sensitive and simple.
Pourshafie
M
M
Pasteur Institute of Iran, Department of Bacteriology, Pasteur Avenue, Tehran, Iran 2Logman Hospital, Department of Infectious Diseases, Tehran, Iran 3Institut Pasteur, Unité des Anaérobies, 25-28 Rue du Dr Roux, F-75724, Paris Cedex 15, France.
Vahdani
P
P
Pasteur Institute of Iran, Department of Bacteriology, Pasteur Avenue, Tehran, Iran 2Logman Hospital, Department of Infectious Diseases, Tehran, Iran 3Institut Pasteur, Unité des Anaérobies, 25-28 Rue du Dr Roux, F-75724, Paris Cedex 15, France.
Popoff
M
M
Pasteur Institute of Iran, Department of Bacteriology, Pasteur Avenue, Tehran, Iran 2Logman Hospital, Department of Infectious Diseases, Tehran, Iran 3Institut Pasteur, Unité des Anaérobies, 25-28 Rue du Dr Roux, F-75724, Paris Cedex 15, France.
D015373:Bacterial Typing Techniques / Q000379:methods*; D001906:Botulism / Q000382:microbiology*; D047013:Clostridium botulinum type A / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D020459:Genes, rRNA; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases