title: Influence of carbon dioxide laser irradiation on the morphology and function of guinea pig cochlea.
abstract: Both experimental and clinical studies have demonstrated that carbon dioxide laser is suitable for stapedotomy. The aim of this study was to investigate morphological, electrophysiological and functional changes in the inner ear after irradiation with CO(2) laser set with different energy parameters. A cochleostomy in the basal cochlear turn of guinea pig cochleae was performed with CO(2) laser of 1, 2 and 3 w, respectively. The cochleae were removed three weeks after laser irradiation. The auditory evoked brainstem response (ABR) was measured before and after laser application and immediately before removal of the cochlea. Immunohistochemical methods were used to examine inducible nitric oxide synthase (iNOS/NOSII) and heat-shock protein 70 (Hsp70) concentrations in the cochlea after laser application. The organ of Corti was studied by scanning electron microscopy. Worse hearing loss was observed in animals receiving higher-power CO(2) laser. These findings correlated with more intense injury of the cochlear ultrastructure and with positive expression of iNOS and Hsp70 in spiral ganglion cells, nerve fibres, supporting cells of the organ of Corti and cells of the spiral ligament. The CO(2) laser as a noncontact procedure is shown to be effective and safe if the total amount of energy is kept within the limits applied in this study. Nitric oxide and stress proteins play important roles in the traumatic mechanism of the inner ear, which are related to hearing loss and injury of the ultrastructure of the inner ear.
authors.affiliation: Department of Otorhinolaryngology, Anhui Provincial Hospital, Anhui Medical University, Hefei City, China. rdd1101@yahoo.com.cn
authors.forename: DongDong
authors.initials: D
authors.lastname: Ren
authors.forename: Jingwu
authors.initials: J
authors.lastname: Sun
authors.forename: Guanglun
authors.initials: G
authors.lastname: Wan
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.forename: Fang
authors.initials: F
authors.lastname: Shen
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000627:therapeutic use*; D003051:Cochlea / Q000033:anatomy & histology / Q000502:physiology / Q000601:surgery*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D053685:Laser Therapy / Q000379:methods*; D008297:Male; D008855:Microscopy, Electron, Scanning; D013201:Stapes Surgery / Q000379:methods
chemical_list: D002245:Carbon Dioxide