Effect of bisphenol A on maturation and quality of semen and eggs in the brown trout, Salmo trutta f. fario.
In the present study male and female brown trout (Salmo trutta f. fario) were exposed to environmentally relevant concentrations of bisphenol A (1.75, 2.40, 5.00 microg l(-1)) during the late prespawning and spawning period and the effect of this contaminant on maturation, quantity and quality of semen and eggs was investigated. In males exposed to estimated BPA concentrations of 1.75 and 2.40 microg l(-1) semen quality was lower than in the control in the beginning of spawning (reduced sperm density, motility rate, and swimming velocity) and in the middle of spawning (reduced swimming velocity, at 2.40 microg l(-1) BPA also reduced sperm motility rate). Therefore, production of high quality semen was restricted to the end of the spawning season and delayed for approximately 4 weeks in comparison to the control. At BPA exposure levels of 5.00 microg l(-1) only one of eight males gave semen of low quality (reduced semen mass, motility rate, and swimming velocity). The percentage of ovulated females was similar for the control group and the groups exposed to estimated BPA concentrations of 1.75 and 2.40 microg l(-1), whereas at 5.00 microg l(-1) BPA females did not ovulate during the investigation. While brown trout of the control group ovulated between the 28 October and 12 November, brown trout exposed to estimated BPA concentrations of 1.75 microg l(-1) BPA ovulated approximately 2 weeks later and brown trout exposed to 2.40 microg l(-1) BPA approximately 3 weeks later. Therefore, the tested BPA concentrations affected the percentage of ovulated females and the time point of ovulation. No effect was observed on the quality of eggs (egg mass, percentile mass increase during hardening, egg fertility).
Lahnsteiner
Franz
F
Department for Organismic Biology, Institute for Zoology, University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria. franz.lahnsteiner@sbg.ac.at
Berger
Beate
B
Kletzl
Manfred
M
Weismann
Thomas
T
D000704:Analysis of Variance; D000818:Animals; D001559:Benzhydryl Compounds; D005260:Female; D008297:Male; D010060:Ovulation / Q000187:drug effects; D010063:Ovum / Q000187:drug effects*; D010636:Phenols / Q000633:toxicity*; D012661:Semen / Q000187:drug effects*; D013997:Time Factors; D014116:Toxicology / Q000295:instrumentation / Q000379:methods; D014337:Trout; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds