title: Molecular characterization of dichloromethane-degrading Hyphomicrobium strains using 16S rDNA and DCM dehalogenase gene sequences.
abstract: A phylogenetic analysis of 6 strains of dichloromethane (DCM) utilizing bacteria was performed. Based on the almost complete 16S rDNA sequence determination, all strains clustered together and showed high sequence similarity to Hyphomicrobium denitrificans, except for the strain MC8b, which is only moderately related to them and probably represents a distinct species. The 16S rDNA-based phylogenetic tree was compared to the one obtained from the DNA sequence data of the dcmA gene coding DCM dehalogenase, the key enzyme of DCM utilization. The topology of the two trees is in good agreement and may suggest an ancient origin of DCM dehalogenase, but also raises questions about the original role of the enzyme.
authors.affiliation: UFZ Centre for Environmental Research Leipzig-Halle, Bioremediation Department, Permoserstrasse 15, 04318 Leipzig, Germany.
authors.forename: Marcell
authors.initials: M
authors.lastname: Nikolausz
authors.forename: Uwe
authors.initials: U
authors.lastname: Kappelmeyer
authors.forename: Ivonne
authors.initials: I
authors.lastname: Nijenhuis
authors.forename: Katja
authors.initials: K
authors.lastname: Ziller
authors.forename: Matthias
authors.initials: M
authors.lastname: Kästner
mesh_terms: D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D020582:Hyphomicrobium / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008190:Lyases / Q000235:genetics*; D008752:Methylene Chloride / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008752:Methylene Chloride; D008190:Lyases; C045224:dichloromethane dehalogenase