title: Identification of prognosis markers in pediatric high-risk acute lymphoblastic leukemia.
abstract: Gene expression profiling may improve the understanding of the biology behind relapse in pediatric acute lymphoblastic leukemia. Using suppression subtractive hybridization (SSH), cDNA concatenated sequencing (CCS), and reverse transcriptase real-time quantitative polymerase chain reaction (RT-RQ-PCR) on high-risk patient samples with nondeterminant chromosomal translocation, the authors identified 3 genes that were significantly overexpressed in the nonrelapsed patients: the calcium/calmodulin-dependent serine protein kinase (CASK), subunit 2 of the cofactor required for SP1 transcriptional activation (CRSP2), and granzyme K (GZMK). The level of expression of these biomarkers may help identify patients with potentially good prognosis within a group otherwise at high risk of relapse.
authors.affiliation: Department of Child Health, Hematology/Oncology Unit, College of Medicine, Sultan Qaboos University, Al-Khod, Oman.
authors.forename: Zakia
authors.initials: Z
authors.lastname: Al-Lamki
authors.forename: Yasser A
authors.initials: YA
authors.lastname: Wali
authors.forename: Shah M
authors.initials: SM
authors.lastname: Wasifuddin
authors.forename: Mathew
authors.initials: M
authors.lastname: Zachariah
authors.forename: Rayhanah
authors.initials: R
authors.lastname: Al-Mjeni
authors.forename: Changping
authors.initials: C
authors.lastname: Li
authors.forename: Shanmugakonar
authors.initials: S
authors.lastname: Muralitharan
authors.forename: Khalsa
authors.initials: K
authors.lastname: Al-Kharusi
authors.forename: Preethi
authors.initials: P
authors.lastname: Gunaratne
authors.forename: Leif
authors.initials: L
authors.lastname: Peterson
authors.forename: Richard
authors.initials: R
authors.lastname: Gibbs
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Gingras
authors.forename: Judith F
authors.initials: JF
authors.lastname: Margolin
mesh_terms: D000293:Adolescent; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism*; D011237:Predictive Value of Tests; D011379:Prognosis; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins