Abnormal expression of genes associated with development and inflammation in the heart of mouse maternal phenylketonuria offspring.
This study describes gene expression in the fetus hearts obtained from mouse model for phenylketonuria. These hearts have cardiovascular disease (CVD). Therefore genes involved in CVD were examined. Several genes associated with heart development and inflammation were found to be altered. In order to investigate whether the abnormal gene expression alters transcription and translation, the levels of troponin mRNA and protein were determined. One step real time RT-PCR showed a reduction in cardiac troponin I, troponin T2 and ryanodine receptor 2. Determination of troponin I and T protein levels showed reduced levels of these proteins. Our results suggest that altered gene expression affects protein production. These changes are likely involved in the cardiovascular defects seen in the mouse.
Matalon
R
R
Department of Pediatrics, The University of Texas Medical Branch (UTMB), Galveston, Texas 77555-0632, USA.
Surendran
S
S
McDonald
J D
JD
Okorodudu
A O
AO
Tyring
S K
SK
Michals-Matalon
K
K
Harris
P
P
D000818:Animals; D003433:Crosses, Genetic; D004195:Disease Models, Animal; D005260:Female; D005318:Fetal Heart / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006331:Heart Diseases / Q000378:metabolism* / Q000473:pathology; D006579:Heterozygote; D006720:Homozygote; D007249:Inflammation / Q000473:pathology*; D051379:Mice; D017042:Phenylketonuria, Maternal / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D040081:Protein Array Analysis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019837:Ryanodine Receptor Calcium Release Channel / Q000032:analysis / Q000235:genetics; D019210:Troponin I / Q000032:analysis / Q000235:genetics; D020107:Troponin T / Q000032:analysis / Q000235:genetics
D012333:RNA, Messenger; D019837:Ryanodine Receptor Calcium Release Channel; D019210:Troponin I; D020107:Troponin T