Structural arrangement of DNA constrained by a cross-linker.
Self-complementary cross-linked oligonucleotides with a disulfide linkage were designed and synthesized. Double helix and hairpin structures were controlled using the different diastereochemistry of phosphoramidate where the cross-linker was introduced. The structures of the strained cross-linked DNAs were estimated by gel mobility, circular dichroism spectra, and melting profiles.
Endo
Masayuki
M
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan. endo@sanken.osaka-u.ac.jp
Majima
Tetsuro
T
D000577:Amides / Q000737:chemistry; D001483:Base Sequence; D002942:Circular Dichroism; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010756:Phosphoric Acids / Q000737:chemistry; D013237:Stereoisomerism; D013696:Temperature
D000577:Amides; D003432:Cross-Linking Reagents; D004220:Disulfides; D009841:Oligonucleotides; D010756:Phosphoric Acids; D004247:DNA; C011067:phosphoramidic acid