Arabitol dehydrogenase as a selectable marker for rice.
Arabitol dehydrogenase has been adapted for use as a plant selectable marker. Arabitol is a five-carbon sugar alcohol that can be used by E. coli strain C, but not by the laboratory K12 strains. The enzyme converts the non-plant-metabolizable sugar arabitol into xylulose, which is metabolized by plant cells. Rice was transformed with a plant-expression-optimized synthetic gene using Biolistic-mediated transformation. Selection on 2.75% arabitol and 0.25% sucrose yielded a transformation efficiency (9.3%) equal to that obtained with hygromycin (9.2%). Molecular analyses showed that the atlD gene was integrated into the rice genome of selected plants and was inherited in a Mendelian manner. This study indicates that arabitol could serve as an effective means of plant selection.
LaFayette
P R
PR
Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Road, Athens, GA 30602-6810, USA.
Kane
P M
PM
Phan
B H
BH
Parrott
W A
WA
D000383:Agriculture / Q000379:methods / Q000639:trends; D019470:Biolistics / Q000379:methods; D002934:Cinnamates / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D018014:Gene Transfer Techniques / Q000639:trends*; D005819:Genetic Markers / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D008967:Molecular Biology / Q000379:methods / Q000639:trends; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D010088:Oxidoreductases / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D013395:Sucrose / Q000378:metabolism / Q000494:pharmacology; D013402:Sugar Alcohols / Q000378:metabolism* / Q000494:pharmacology; D014170:Transformation, Genetic / Q000187:drug effects / Q000235:genetics
D002934:Cinnamates; D005819:Genetic Markers; D013402:Sugar Alcohols; D006921:Hygromycin B; C026273:hygromycin A; D013395:Sucrose; D010088:Oxidoreductases; C014999:arabitol