title: The study of 3-dimensional structures of IgG with atomic force microscopy.
abstract: OBJECTIVE
  To detect 3-dimensional images of anti-N-methyl-D-aspartate receptor Nr1 (NMDAr1) polycolonal IgG affixed on mica in physiological environment.
  
  
  METHODS
  The images and data were obtained from a contact mode and commercial Si3N4 probed tip by using atomic force microscope (AFM).
  
  
  RESULTS
  The anti-NMDAr1 polycolonal IgG has a characteristic structure described as an ellipse spherical shape of 136.4 A x 62.8 A x 26.1 A. On the section of the ellipse edge there were two peaks about 13 nm in width.
  
  
  CONCLUSIONS
  Using AFM to investigate biomacromolecule can make us deeply understand the structure of IgG, which will instruct us to detect the membrane receptor protein as a labelling agent.
authors.affiliation: Department of Neurosurgery, Zhujiang Hospital, South Medical University, Guangzhou 510282, China. yu-yg@tom.com
authors.forename: Yi-gang
authors.initials: YG
authors.lastname: Yu
authors.forename: Ru-xiang
authors.initials: RX
authors.lastname: Xu
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Jiang
authors.forename: Yi-quan
authors.initials: YQ
authors.lastname: Ke
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates; D017979:Gold Colloid; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D016194:Receptors, N-Methyl-D-Aspartate / Q000737:chemistry*
chemical_list: D000538:Aluminum Silicates; D017979:Gold Colloid; D007074:Immunoglobulin G; D016194:Receptors, N-Methyl-D-Aspartate; C011934:mica