Two fast methods for detection of Y-microdeletions.
OBJECTIVE
To test two recently available commercial kits: the new Promega Y Chromosome Deletion Detection System 2.0 kit and the Bird-Set kits (Y Chromosome UE and Extension).


METHODS
A comparative technical study.


METHODS
Male infertility clinic.


METHODS
Twelve various Y chromosome microdeleted patients were included in the present study: two AZFa deleted, two AZFb deleted, four AZFb+c deleted, three AZFc deleted, and one AZF a+b+c deleted.


METHODS
DNA samples were tested with both the Promega kit and the Bird-Set kits.


METHODS
Electrophoresis and analysis comparison on a bioanalyzer.


RESULTS
Both kits (Promega 2.0 and Bird Y Chromosome UE) confirmed the 12 Y deletions. Both kits (Bird Extension and Promega 2.0) were able to determine the length of the 12 microdeletions with various differences. The new Promega 2.0 kit was considerably improved, with the addition of two sequenced tag sites (STS) in AZFa (sY86 and sY84) and the deletion of some (sY239 and sY153) but not all supernumerary inadequate STS.


CONCLUSIONS
The two new commercially available kits use two different protocols to detect Y chromosome microdeletions. The Promega kit is a one-step diagnostic test. The Bird diagnostic test uses a two-step protocol. Both kits offer relevant methods such as standardization and ready-to-use mixes. However, both kits need further evaluation under routine conditions using nontested DNA samples.
Aknin-Seifer
Isabelle E
IE
Laboratoire de Biologie de la Reproduction, GIMAP, Saint Etienne, France.
Touraine
Renaud L
RL
Faure
Anne-Karen
AK
Fellmann
Florence
F
Chouteau
Jacques
J
Levy
Rachel
R
D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004586:Electrophoresis / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data