title: Maternal and fetal single nucleotide polymorphisms in the epoxide hydrolase and gluthatione S-transferase P1 genes are not associated with pre-eclampsia in the Coloured population of the Western Cape, South Africa.
abstract: Oxidative stress is thought to play an important role in the pathophysiology of pre-eclampsia. A defect in certain enzymes responsible for detoxification may cause prolonged exposure to reactive by-products and contribute to maternal endothelial as well as placental damage. Two polymorphisms affecting the function of the biotransformation enzymes epoxide hydrolase and glutathione S-transferase P1 were shown previously to be associated with pre-eclampsia in a Dutch population. The aim of this study was to determine if these two polymorphisms (maternal or fetal) contribute to pre-eclampsia in an anthropologically distinct population (the Western Cape region of South Africa) with a high incidence of the disease. Genomic DNA of mother - infant pairs with severe pre-eclampsia (n = 144), a population control group (n = 156) and control mother - infant pairs with uncomplicated pregnancy outcome (n = 45) were analysed for the EPHX and GSTP1 polymorphisms by polymerase chain reaction amplification and restriction enzyme digestion. Each polymorphism had a similar distribution in case and control subjects (mother and infant). The Val105/Val105 genotype of GSTP1 occurred at a higher frequency than reported for other populations. Neither maternal nor fetal EPHX Tyr113His and GSTP1 Ile105Val polymorphisms appear to contribute significantly to the pathophysiology of pre-eclampsia in the Coloured population of the Western Cape region of South Africa.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Stellenbosch, South Africa. gsgeb@sun.ac.za
authors.forename: G S
authors.initials: GS
authors.lastname: Gebhardt
authors.forename: W H M
authors.initials: WH
authors.lastname: Peters
authors.forename: R
authors.initials: R
authors.lastname: Hillermann
authors.forename: H J
authors.initials: HJ
authors.lastname: Odendaal
authors.forename: K
authors.initials: K
authors.lastname: Carelse-Tofa
authors.forename: M T M
authors.initials: MT
authors.lastname: Raijmakers
authors.forename: E A P
authors.initials: EA
authors.lastname: Steegers
mesh_terms: D000483:Alleles; D044383:Black People / Q000235:genetics; D004247:DNA / Q000032:analysis; D004851:Epoxide Hydrolases / Q000235:genetics*; D005091:Exons; D005260:Female; D005333:Fetus / Q000201:enzymology*; D005787:Gene Frequency; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006639:Histidine; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D013019:South Africa
chemical_list: D006639:Histidine; D004247:DNA; D005982:Glutathione Transferase; D004851:Epoxide Hydrolases