[Analysis of FcgammaRIIIb genotype of periodontal disease in the Han nationality in China].
OBJECTIVE
The purpose of the present study was to compare FcgammaRIIIb genotype of aggressive periodontitis (AgP) with chronic periodontitis (CP) and control group (Cont) in the Han nationality in China.


METHODS
The classification, which was applied in this study, was presented and discussed at the 1999 International Workshop for the Classification of the Periodontal Disease organized by the American Academy of Periodontology (AAP). 23 patients with aggressive periodontitis, including 20 with chronic periodontitis according to the established clinical criteria, and 22 matched healthy controls was included in this study. Peripheral blood samples were taken from all patients and controls, then DNA was isolated from blood using DNA purification kit. And FcgammaRIIIb genotyping was determined by PCR and DNA sequencing. The different results of genotype in all three groups were analysed by chi(2) test.


RESULTS
FcgammaRIIIb-NA1, -NA2 (old definition) frequencies didn't equal 1.00 in all 3 groups, and also without -SH, -NA(NULL) genotype. At the same time, many new allele of NA1 and NA2 were found in all groups. However, there were no rules of new alleles in each group and between the groups, no statistical significance as well. The -NA1 of FcgammaRIIIb (new definition) frequency was 0.77, and that of -NA2 (new definition) was 0.23 in this study.


CONCLUSIONS
Including FcgammaRIIIb-NA1 and -NA2, five alternate allele of -NA1 (-NA1*01b, -NA1*02a, -NA1*02b, -NA1*03a and-NA1*03b) and three alternate allele of -NA2(-NA2*02, -NA2*03 and -NA2*04) were described. The results suggest that FcgammaRIIIb may not be a risk indicator for the susceptibility of periodontitis in the Han nationality of China.
Xie
Yu-Feng
YF
Department of Oral Medicine, Ninth People's Hospital, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Shu
Rong
R
Cheng
Lan
L
D010520:Aggressive Periodontitis / Q000235:genetics*; D000483:Alleles; D044466:Asian People; D016022:Case-Control Studies; D005006:Ethnicity; D058851:GPI-Linked Proteins / Q000235:genetics; D005838:Genotype; D006801:Humans; D010510:Periodontal Diseases / Q000235:genetics*; D017452:Receptors, IgG / Q000235:genetics*
C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D017452:Receptors, IgG