Lipophilic porphyrin microparticles induced by AOT reverse micelles: a fluorescence lifetime imaging study.
Fluorescence Lifetime Imaging Microscopy (FLIM) technique was applied to investigate the fluorescence dynamics and structural features of large colloidal aggregates of meso-tetra(N-dodecyl-4-amino sulfonyl-phenyl)porphyrin (PC12) induced by Sodium 1,4-bis(2-ethyl hexyl)sulfosuccinate (AOT) reverse micelles. The aggregate's particle sizes (down to 1 microm) obtained from the confocal fluorescence images matched with the particle sizes measured in the images obtained from Scanning Electron Microscopy (SEM). The fluorescence decays for those aggregates in the micro spatial domain show triexponential fluorescence lifetimes (tau1 approximately 12 ns, tau2 approximately 3 ns and tau3 approximately 1 ns) which are independent of the aggregate's size.
Togashi
Denisio M
DM
Centro de Química Estrutural, Complexo I-Instituto Superior Técnico, 1049-001 Lisboa, Portugal.
Costa
Sílvia M B
SM
Sobral
Abílio J F N
AJ
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003102:Colloids / Q000737:chemistry; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation* / Q000379:methods*; D008823:Micelles; D008855:Microscopy, Electron, Scanning / Q000379:methods; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D011166:Porphyrins / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013499:Surface Properties
D003102:Colloids; D008823:Micelles; D011166:Porphyrins; C510459:meso-tetra(N-dodecyl-4-amino sulfonyl-phenyl)porphyrin; D004143:Dioctyl Sulfosuccinic Acid