[Analysis of volatile components from the flowers of Chrysanthemum morifolium by GC-MS with solid-phase microextraction].
OBJECTIVE
To study a method for extraction and analysis of volatile components from Chrysanthemum morifolium 'gonghuangjv' cv. nov (CM GHJ) and C. morifolium 'gongbaijv' cv. nov (CM GBJ) by solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS).


METHODS
The volatile components were extracted in different temperature, different balance period and different extraction fiber using head space solid-phase microextraction (HS-SPME), and were identified by CGC-MS. The variety in integral area of each component was observed in different conditions and its relative content was determined by normalization of area.


RESULTS
The better condition of SPME for C. morifolium was that the sample was extracted using 100 microm polydimethylsiloxane (PDMS) extraction fiber after it had been balanced for 6 hours at 75 degrees C. 53 components from CM GHJ and CM GBJ were identified, and there were 35 same components in CM GHJ and CM GBJ.


CONCLUSIONS
HS-SPME-GC-MS is convenient, rapid and reliable for analysis of volatile components in C. morifolium.
Zhou
Hai-mei
HM
Henan University of Science and Technology, Luoyang 471003, China. lyzhm@xin-huanet.com
Xie
Pei-shan
PS
Wang
Wan-hui
WH
Ma
Jin-qi
JQ
Li
Pu
P
D031202:Chrysanthemum / Q000737:chemistry* / Q000145:classification; D035264:Flowers / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009822:Oils, Volatile / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification
D009822:Oils, Volatile