title: Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition.
abstract: Pseudomonas syringae pv. phaseolicola, a gram-negative bacterial plant pathogen, is the causal agent of halo blight of bean. In this study, we report on the genome sequence of P. syringae pv. phaseolicola isolate 1448A, which encodes 5,353 open reading frames (ORFs) on one circular chromosome (5,928,787 bp) and two plasmids (131,950 bp and 51,711 bp). Comparative analyses with a phylogenetically divergent pathovar, P. syringae pv. tomato DC3000, revealed a strong degree of conservation at the gene and genome levels. In total, 4,133 ORFs were identified as putative orthologs in these two pathovars using a reciprocal best-hit method, with 3,941 ORFs present in conserved, syntenic blocks. Although these two pathovars are highly similar at the physiological level, they have distinct host ranges; 1448A causes disease in beans, and DC3000 is pathogenic on tomato and Arabidopsis. Examination of the complement of ORFs encoding virulence, fitness, and survival factors revealed a substantial, but not complete, overlap between these two pathovars. Another distinguishing feature between the two pathovars is their distinctive sets of transposable elements. With access to a fifth complete pseudomonad genome sequence, we were able to identify 3,567 ORFs that likely comprise the core Pseudomonas genome and 365 ORFs that are P. syringae specific.
authors.affiliation: The Institute for Genomic Research, 9712 Medical Center Dr., Rockville, MD 20850, USA.
authors.forename: Vinita
authors.initials: V
authors.lastname: Joardar
authors.forename: Magdalen
authors.initials: M
authors.lastname: Lindeberg
authors.forename: Robert W
authors.initials: RW
authors.lastname: Jackson
authors.forename: Jeremy
authors.initials: J
authors.lastname: Selengut
authors.forename: Robert
authors.initials: R
authors.lastname: Dodson
authors.forename: Lauren M
authors.initials: LM
authors.lastname: Brinkac
authors.forename: Sean C
authors.initials: SC
authors.lastname: Daugherty
authors.forename: Robert
authors.initials: R
authors.lastname: Deboy
authors.forename: A Scott
authors.initials: AS
authors.lastname: Durkin
authors.forename: Michelle Gwinn
authors.initials: MG
authors.lastname: Giglio
authors.forename: Ramana
authors.initials: R
authors.lastname: Madupu
authors.forename: William C
authors.initials: WC
authors.lastname: Nelson
authors.forename: M J
authors.initials: MJ
authors.lastname: Rosovitz
authors.forename: Steven
authors.initials: S
authors.lastname: Sullivan
authors.forename: Jonathan
authors.initials: J
authors.lastname: Crabtree
authors.forename: Todd
authors.initials: T
authors.lastname: Creasy
authors.forename: Tanja
authors.initials: T
authors.lastname: Davidsen
authors.forename: Dan H
authors.initials: DH
authors.lastname: Haft
authors.forename: Nikhat
authors.initials: N
authors.lastname: Zafar
authors.forename: Liwei
authors.initials: L
authors.lastname: Zhou
authors.forename: Rebecca
authors.initials: R
authors.lastname: Halpin
authors.forename: Tara
authors.initials: T
authors.lastname: Holley
authors.forename: Hoda
authors.initials: H
authors.lastname: Khouri
authors.forename: Tamara
authors.initials: T
authors.lastname: Feldblyum
authors.forename: Owen
authors.initials: O
authors.lastname: White
authors.forename: Claire M
authors.initials: CM
authors.lastname: Fraser
authors.forename: Arun K
authors.initials: AK
authors.lastname: Chatterjee
authors.forename: Sam
authors.initials: S
authors.lastname: Cartinhour
authors.forename: David J
authors.initials: DJ
authors.lastname: Schneider
authors.forename: John
authors.initials: J
authors.lastname: Mansfield
authors.forename: Alan
authors.initials: A
authors.lastname: Collmer
authors.forename: C Robin
authors.initials: CR
authors.lastname: Buell
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D044224:Pseudomonas syringae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D013045:Species Specificity; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial