title: Safety and efficacy of Mycoplasma gallisepticum TS-11 vaccine for the protection of layer pullets against challenge with virulent M. gallisepticum R-strain.
abstract: Mycoplasma gallisepticum TS-11 vaccine was studied for its safety and protective ability in 49-day-old M. gallisepticum-free and Mycoplasma synoviae-free commercial Tetra SL layer chickens. Sixty birds were distributed into four groups: 15 were unvaccinated but were challenged with M. gallisepticum R-strain, 15 were vaccinated by eye drop and then challenged with virulent M. gallisepticum R-strain 4 weeks post vaccination, 15 were designated as controls without vaccination and challenge, and 15 received TS-11 vaccine but no challenge. Based on the post-challenge clinical signs, body weight gain, gross pathological examination of air sacs and peritoneum, histological examination of the trachea, lung, spleen and liver, and reisolation of mycoplasmas from inner organs, the TS-11 vaccine is safe and does not produce clinical signs, a major decrease of body weight gain or pathological lesions. Vaccination induced a slight serological response to M. gallisepticum antigen in serum plate agglutination and blocking enzyme-linked immunosorbent assay tests and prevented development clinical signs of airsacculitis, peribronchitis and interstitial pneumonia on M. gallisepticum challenge.
authors.affiliation: Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Hungária krt. 2, H-1143, Budapest, Hungary. bjudit@novell.vmri.hu
authors.forename: Judit
authors.initials: J
authors.lastname: Bíró
authors.forename: J
authors.initials: J
authors.lastname: Povazsán
authors.forename: L
authors.initials: L
authors.lastname: Korösi
authors.forename: R
authors.initials: R
authors.lastname: Glávits
authors.forename: L
authors.initials: L
authors.lastname: Hufnagel
authors.forename: L
authors.initials: L
authors.lastname: Stipkovits
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000009:adverse effects; D001991:Bronchitis / Q000382:microbiology / Q000662:veterinary; D002645:Chickens / Q000382:microbiology*; D005260:Female; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D009092:Mucous Membrane / Q000473:pathology; D009175:Mycoplasma Infections / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D045686:Mycoplasma gallisepticum / Q000276:immunology / Q000472:pathogenicity*; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D014132:Trachea / Q000473:pathology; D014774:Virulence; D015430:Weight Gain
chemical_list: D001428:Bacterial Vaccines