title: Alterations in the colonic flora and intestinal permeability and evidence of immune activation in chronic constipation.
abstract: BACKGROUND
  Disturbances in bowel function in chronic constipation could result in changes in the colonic flora and lead to disordered immunity and to decreased resistance to pathogenic flora.
  
  
  OBJECTIVE
  To investigate systemic immunity, the faecal flora and intestinal permeability in patients with chronic constipation, under basal conditions and following therapy with the laxative Bisacodyl.
  
  
  METHODS
  Intestinal permeability, faecal flora analysis, T- and B-lymphocyte numbers, T-cell subpopulations, lymphocyte proliferation, phagocytosis, intracellular killing of Staphylococcus aureus by neutrophils, as well as circulating levels of immunoglobulins, immune complexes and antibacterial antibodies were assessed in 57 patients with functional constipation. In 12 patients with severely delayed transit, investigations were repeated following therapy with Bisacodyl.
  
  
  RESULTS
  Ovalbumin concentrations, in serum, were higher in constipated patients (28.2+/-4.1 ng/ml versus 1.0+/-0.4 ng/ml, p < 0.05). Elevated counts of CD3+, CD4+, CD25+ cells, increased spontaneous proliferation of lymphocytes, elevated titres of antibodies to Escherichia coli and S. aureus, diminished counts of CD72+ B cells, diminished lymphocyte proliferation under phytohemagglutinin (PHA) stimulation and a diminished phagocytic index for both neutrophils and monocytes were found in the constipated patients. Concentrations of Bifidobacterium and Lactobacillus were significantly lower in constipated patients; potentially pathogenic bacteria and/or fungi were increased. Therapy with Bisacodyl resulted in normalisation of the faecal flora, a reduction in ovalbumin concentration and return towards normal for certain immunologic parameters.
  
  
  CONCLUSIONS
  Constipation is associated with striking changes in the faecal flora, intestinal permeability and the systemic immune response. Relief of constipation tends to normalise these findings suggesting that these changes are secondary to, rather than a cause of, constipation.
authors.affiliation: State Scientific Center for Coloproctology, Moscow, Russia.
authors.forename: I L
authors.initials: IL
authors.lastname: Khalif
authors.forename: E M M
authors.initials: EM
authors.lastname: Quigley
authors.forename: E A
authors.initials: EA
authors.lastname: Konovitch
authors.forename: I D
authors.initials: ID
authors.lastname: Maximova
mesh_terms: D001644:Bifidobacterium / Q000378:metabolism; D001726:Bisacodyl / Q000494:pharmacology / Q000627:therapeutic use; D002400:Cathartics / Q000494:pharmacology / Q000627:therapeutic use; D002463:Cell Membrane Permeability; D002908:Chronic Disease; D003106:Colon / Q000276:immunology* / Q000382:microbiology*; D003248:Constipation / Q000276:immunology* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005772:Gastrointestinal Transit; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D007778:Lactobacillus / Q000378:metabolism; D018655:Lymphocyte Count; D008297:Male; D010047:Ovalbumin / Q000097:blood; D010587:Phagocytosis
chemical_list: D002400:Cathartics; D001726:Bisacodyl; D010047:Ovalbumin