Molecular assembly of superquenchers in signaling molecular interactions.
We have designed a novel molecular assembly of quencher molecules to form superquenchers with excellent quenching efficiency. The superquencher can be engineered as desired by assembling different types and different numbers of quencher molecules. By labeling a superquencher to a molecular beacon, a 320-fold enhancement of fluorescent signal was achieved, compared to about 14-fold from a molecular beacon prepared with the same monomer quencher. Our molecular assembly approach can effectively improve the sensitivity of a variety of fluorescent assays and can be widely useful for molecular interaction studies.
Yang
Chaoyong James
CJ
Center for Research at Bio/nano Interface, Department of Chemistry, UF Genetics Institute and Shands Cancer Center, University of Florida, Gainesville, Florida 32611, USA.
Lin
Hui
H
Tan
Weihong
W
D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015336:Molecular Probe Techniques; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013696:Temperature
D015342:DNA Probes; D005456:Fluorescent Dyes; D009841:Oligonucleotides