title: Allosteric modulation of the nicotinic acetylcholine receptor by physostigmine.
abstract: The action of physostigmine on mouse muscle nicotinic acetylcholine receptor expressed in the COS-7 cell line was studied by the patch-clamp technique. Physostigmine accelerated, by allosteric modulation, the rate of desensitization of whole cell currents induced by acetylcholine and decreased the maximal amplitude in concentration-dependent manner.
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.
authors.forename: Lucie
authors.initials: L
authors.lastname: Svobodová
authors.forename: Jan
authors.initials: J
authors.lastname: Krusek
authors.forename: Tomás
authors.initials: T
authors.lastname: Hendrych
authors.forename: Frantisek
authors.initials: F
authors.lastname: Vyskocil
mesh_terms: D000494:Allosteric Regulation / Q000187:drug effects*; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D010830:Physostigmine / Q000494:pharmacology*; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002800:Cholinesterase Inhibitors; D011978:Receptors, Nicotinic; D010830:Physostigmine