Inhibitory effect of some selected nutraceutic herbs on LDL glycation induced by glucose and glyoxal.
Anti-LDL glycative agents were investigated using aqueous extracts of Psidium guajava L. (PE), Toona sinensis Roem. (TE), Momordica charantia L. (ME) and Graptopetalum paragugayene E. Walther (GE). Concentrations of extracts 0.01-0.625 mg/mL, low density lipoprotein (LDL; 100 microg protein/mL) and inducers glucose (400 mM) and glyoxal (2.5 mM) were incubated at 37 degrees C. Evaluation parameters involved the thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD), relative electrophoretic mobility (REM), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capability and total polyphenolic content. Results for anti-TBARS efficiency (in%) were PE (75.77), TE (75.10), ME (68.81) and GE (19.81) at 0.5 mg/mL, respectively, when induced by glucose; 36.68, 35.60, 32.62 and inactive, respectively, by glyoxal. The lag times for CD formation (in min) were: 289 and 125 by PE and TE, respectively, comparing to the control (45). REM was 1.6 with respect to PE (0.1 mg/mL) compared to the control (4.2). PE at 0.01 mg/mL effectively inhibited with 63.45% efficiency on AGEs induced by glucose. We conclude that PE virtually is a potent antiglycative agent, which can be of great value in the preventive glycation-associated cardiovascular and neurodegenerative diseases.
Hsieh
Chiu-Lan
CL
Department of Food and Nutrition, Shalu County, Taichung, Taiwan, ROC.
Lin
Yuh-Charn
YC
Ko
Wang-Sheng
WS
Peng
Chiung-Hui
CH
Huang
Chien-Ning
CN
Peng
Robert Y
RY
D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006031:Glycosylation; D006037:Glyoxal / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D010946:Plants, Medicinal; D059808:Polyphenols; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D005419:Flavonoids; D016166:Free Radical Scavengers; D017127:Glycation End Products, Advanced; D007004:Hypoglycemic Agents; D008077:Lipoproteins, LDL; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D017392:Thiobarbituric Acid Reactive Substances; D006037:Glyoxal; D005947:Glucose