The nucleotide sequence and a first generation gene transfer vector of species B human adenovirus serotype 3.
Human adenovirus (Ad) serotype 3 causes respiratory infections. It is considered highly virulent, accounting for about 13% of all Ad isolates. We report here the complete Ad3 DNA sequence of 35,343 base pairs (GenBank accession DQ086466). Ad3 shares 96.43% nucleotide identity with Ad7, another virulent subspecies B1 serotype, and 82.56 and 62.75% identity with the less virulent species B2 Ad11 and species C Ad5, respectively. The genomic organization of Ad3 is similar to the other human Ads comprising five early transcription units, E1A, E1B, E2, E3, and E4, two delayed early units IX and IVa2, and the major late unit, in total 39 putative and 7 hypothetical open reading frames. A recombinant E1-deleted Ad3 was generated on a bacterial artificial chromosome. This prototypic virus efficiently transduced CD46-positive rodent and human cells. Our results will help in clarifying the biology and pathology of adenoviruses and enhance therapeutic applications of viral vectors in clinical settings.
Sirena
Dominique
D
Institute of Molecular Biology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
Ruzsics
Zsolt
Z
Schaffner
Walter
W
Greber
Urs F
UF
Hemmi
Silvio
S
D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D006224:Cricetinae; D004279:DNA, Viral / Q000235:genetics; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016679:Genome, Viral; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D051916:Membrane Cofactor Protein / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000235:genetics; D012703:Serotyping; D014161:Transduction, Genetic; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence / Q000235:genetics
D004279:DNA, Viral; D051916:Membrane Cofactor Protein; D011994:Recombinant Proteins; D014764:Viral Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins