title: The effect of a mouth rinse containing phenolic compounds on plaque formation and developing gingivitis.
abstract: BACKGROUND
  The clinical effect of Listerine, a mouth rinse containing a mixture of phenolic compounds, is ascribed to its bactericidal properties. However, phenolic compounds are also known to interfere with the inflammatory process.
  
  
  OBJECTIVE
  The purpose of this clinical trial was to evaluate the effect of regular mouth rinsing with Listerine on plaque and gingivitis during a 2-week period of no mechanical oral hygiene.
  
  
  METHODS
  Twenty-one subjects were recruited for the study. On Day 0 of each 2-week experimental period, the participants were told to abstain from all mechanical plaque-control measures but to rinse twice a day with 10 ml of the assigned solution (test: Listerine, positive control:0.1% chlorhexidine (CHX), negative control: saline) for 60 s. Each experimental period was preceded by a 2-week period including oral hygiene instruction, scaling and professional mechanical tooth cleaning. Examinations included assessments of plaque and gingivitis (Days 0 and 14), sampling of plaque and collection of gingival crevicular fluid (GCF) (Days 0, 7 and 14). From the supragingival plaque samples, six different morphotypes of bacteria were counted using dark-field microscopy. The GCF collected was analysed with respect to the content of lactoferrin and albumin.
  
  
  RESULTS
  During the experimental periods, it was observed that significantly less plaque formed and less gingivitis developed when the participants rinsed with the Listerine mouthwash than with saline solution. However, significantly more plaque formed during the Listerine than during the CHX rinse period, while there was no significant difference in the development of gingival bleeding between the Listerine and the CHX rinse regimens. Significantly smaller proportions of motile rods and fusiforms were found in the List and CHX groups than in the control (Ctrl) group. The increase of the lactoferrin/albumin ratio in the List group was significantly smaller than that in the Ctrl group but significantly larger than in the CHX group.
  
  
  CONCLUSIONS
  It was suggested that the effect of Listerine on gingivitis is more pronounced than on plaque formation. This indicates that the phenolic compound may have anti-inflammatory effects.
authors.affiliation: Faculty of Odontology, Department of Periodontology, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sekino
authors.forename: Per
authors.initials: P
authors.lastname: Ramberg
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000627:therapeutic use; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000188:drug therapy*; D004338:Drug Combinations; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D005891:Gingivitis / Q000188:drug therapy*; D006801:Humans; D009067:Mouthwashes / Q000627:therapeutic use*; D010636:Phenols / Q000627:therapeutic use; D012459:Salicylates / Q000627:therapeutic use*; D016037:Single-Blind Method; D013729:Terpenes / Q000627:therapeutic use*
chemical_list: D004338:Drug Combinations; D009067:Mouthwashes; D010636:Phenols; D012459:Salicylates; D013729:Terpenes; C057473:Listerine; C010882:chlorhexidine gluconate; D002710:Chlorhexidine