title: Depletion of resident Chlamydia pneumoniae through leukoreduction by filtration of blood for transfusion.
abstract: Current studies indicate that a significant percentage of healthy blood donors carry Chlamydia pneumoniae in their blood. Although the clinical significance of such findings is unknown, eradication of such bacteria from blood components may contribute to transfusion safety. Deletion of C. pneumoniae in Red Blood Cell (RBC) units was accomplished through leukoreduction by filtration. The presence of bacteria in RBC units before and after leukoreduction was assessed by real-time PCR using primers specific for C. pneumoniae 16S rRNA. The eluates of filters used for leukoreduction were also assessed by PCR and immunostaining with fluorescein isothiocyanate-conjugated chlamydial monoclonal antibodies specific for C. pneumoniae determination. Nineteen of 30 RBC units tested showed the presence of C. pneumoniae DNA. Leukofiltration resulted in a marked reduction of leukocytes as well as C. pneumoniae in terms of bacterial number and positive rate for the bacteria. The eluates of filters showed trapped bacteria determined by both PCR and immunostaining assays. Thus, leukoreduction with a filter is an effective method to significantly reduce resident C. pneumoniae levels in RBC components but may not be completely sufficient for total eradication of this pathogen.
authors.affiliation: Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa, 33612, USA.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ikejima
authors.forename: Herman
authors.initials: H
authors.lastname: Friedman
authors.forename: German F
authors.initials: GF
authors.lastname: Leparc
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D000328:Adult; D000368:Aged; D001782:Blood Donors; D001803:Blood Transfusion; D002690:Chlamydia Infections / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D004912:Erythrocytes / Q000382:microbiology*; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D047589:Leukocyte Reduction Procedures / Q000379:methods*; D007962:Leukocytes / Q000166:cytology / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004269:DNA, Bacterial