title: Identification of new CNS-resident macrophage subpopulation molecular markers for the discrimination with murine systemic macrophages.
abstract: A controversial issue in neurobiology concerns the respective functions of central nervous system (CNS)-resident macrophages and systemic infiltrating macrophages morphologically and phenotypically similar during most of CNS injury processes. In a previous work, we isolated sixteen mRNAs differentially expressed between two microglial EOC clones. By studying their pattern of expression, we found that three of them were not expressed in peripheral macrophages, even after stimulation with IFNgamma, TNFalpha or IL10. These three molecules are physiologically expressed by murine adult microglia and could be used to evaluate in vivo their discriminative potential toward CNS-infiltrating macrophages during inflammatory events.
authors.affiliation: Laboratoire de Biologie Moléculaire, Immunologie et Thérapeutique des Cancers (UPRES EA 3140), CHU, bâtiment Monteclair, 4 rue Larrey, 49033 ANGERS cedex 01, France.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Donnou
authors.forename: Sylvain
authors.initials: S
authors.lastname: Fisson
authors.forename: Dominique
authors.initials: D
authors.lastname: Mahe
authors.forename: Alicia
authors.initials: A
authors.lastname: Montoni
authors.forename: Dominique
authors.initials: D
authors.lastname: Couez
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000145:classification / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D015152:Blotting, Northern / Q000379:methods; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology*; D018076:DNA, Complementary / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D016753:Interleukin-10 / Q000494:pharmacology; D008262:Macrophage Activation / Q000502:physiology*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D017628:Microglia / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D018076:DNA, Complementary; D005904:Glial Fibrillary Acidic Protein; D016898:Interferon-alpha; D012333:RNA, Messenger; D016753:Interleukin-10; D007371:Interferon-gamma