title: RSEP1 is a novel gene with functional involvement in neuropathic pain behaviour.
abstract: Neuropathic pain from nerve injury by trauma, disease or surgery often causes prolonged suffering. To explore the molecular mechanisms that underlie neuropathic pain, we used mRNA from the L4--5 segments of the lumbar spinal cord of rats with chronic constriction injury (CCI)-induced neuropathic pain, and differentially screened a cDNA library from the rat brain. A novel gene, termed RSEP1 (Rat Spinal cord Expression Protein 1), was identified. Northern blots revealed that RSEP1 was expressed mainly in the central nervous system including the cerebral cortex, hippocampus, brainstem and spinal cord, as well as in the kidney and ovary. In situ hybridization showed a high level of RSEP1 expression in the CA1, CA3 and dentate gyrus regions of the hippocampus and the small sensory neurons in the dorsal horn, as well as the large neurons in the ventral horn of the spinal cord. Intrathecal injection of RSEP1 antisense oligonucleotide into the spinal cord lumbar enlargement attenuated neuropathic pain behaviours in CCI rats, suggesting a functional involvement of RSEP1 in neuropathic pain.
authors.affiliation: Laboratory of Molecular Cell Biology, Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Xidao
authors.initials: X
authors.lastname: Wang
authors.forename: Yuqiu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lingwei
authors.initials: L
authors.lastname: Kong
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Xie
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Lin
authors.forename: Ning
authors.initials: N
authors.lastname: Guo
authors.forename: Judith A
authors.initials: JA
authors.lastname: Strong
authors.forename: Johanna T A
authors.initials: JT
authors.lastname: Meij
authors.forename: Zhiqi
authors.initials: Z
authors.lastname: Zhao
authors.forename: Naihe
authors.initials: N
authors.lastname: Jing
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D015152:Blotting, Northern / Q000379:methods; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006930:Hyperalgesia / Q000188:drug therapy; D017403:In Situ Hybridization / Q000379:methods; D007211:Indoles / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010146:Pain / Q000209:etiology / Q000378:metabolism*; D010147:Pain Measurement / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D020426:Sciatic Neuropathy / Q000150:complications / Q000378:metabolism*; D017422:Sequence Analysis, DNA / Q000379:methods; D020539:Sequence Analysis, Protein / Q000379:methods; D013997:Time Factors
chemical_list: C506807:BLOC1S2 protein, rat; D007211:Indoles; D009419:Nerve Tissue Proteins; D020319:Oligodeoxyribonucleotides, Antisense; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C007293:DAPI