title: Production of exopolysaccharide from mycelial culture of Grifola frondosa and its inhibitory effect on matrix metalloproteinase-1 expression in UV-irradiated human dermal fibroblasts.
abstract: Exopolysaccharide (EPS) was prepared by submerged mycelial culture of a newly isolated mushroom Grifola frondosa HB0071 in a 5-l stirred-tank fermenter. This fungus produced a high concentration of biomass (24.8 gl(-1) at day 4), thereby achieving high EPS concentration (7.2 gl(-1) at day 4). EPS was proven to be a proteoglycan consisting of 85.6% carbohydrates (mostly glucose) and 7.3% proteins with a molecular weight of 1.0 x 10(6) Da. The photoprotective potential of EPS was tested in human dermal fibroblasts (HDF) exposed to ultraviolet-A (UVA) light. It was revealed that EPS had an inhibitory effect on human interstitial collagenase (matrix metalloproteinase, MMP-1) expression in UVA-irradiated HDF without any significant cytotoxicity. The treatment of UVA-irradiated HDF with EPS resulted in a dose-dependent decrease in the expression level of MMP-1 mRNA (by maximum 61.1% at an EPS concentration 250 microgml(-1)). These results suggest that EPS obtained from mycelial culture of G. frondosa HB0071 may contribute to inhibitory action in photoaging skin by reducing the MMP 1-related matrix degradation system.
authors.affiliation: R and D Center, Hanbul Cosmetics Co., Chungbuk 369-830, Republic of Korea.
authors.forename: Jun Tae
authors.initials: JT
authors.lastname: Bae
authors.forename: Gwan Sub
authors.initials: GS
authors.lastname: Sim
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Lee
authors.forename: Bum Chun
authors.initials: BC
authors.lastname: Lee
authors.forename: Hyeong Bae
authors.initials: HB
authors.lastname: Pyo
authors.forename: Tae Boo
authors.initials: TB
authors.lastname: Choe
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Yun
mesh_terms: D000363:Agaricales / Q000201:enzymology / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000187:drug effects / Q000378:metabolism*; D025282:Mycelium / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis* / Q000494:pharmacology*; D012867:Skin / Q000166:cytology; D014466:Ultraviolet Rays
chemical_list: D011134:Polysaccharides; D020781:Matrix Metalloproteinase 1