title: Resolving mechanisms of toxicity while pursuing ecotoxicological relevance?
abstract: In this age of modern biology, aquatic toxicological research has pursued mechanisms of action of toxicants. This has provided potential tools for ecotoxicologic investigations. However, problems of biocomplexity and issues at higher levels of biological organization remain a challenge. In the 1980s and 1990s and continuing to a lesser extent today, organisms residing in highly contaminated field sites or exposed in the laboratory to calibrated concentrations of individual compounds were carefully analyzed for their responses to priority pollutants. Correlation of biochemical and structural analyses in cultured cells and tissues, as well as the in vivo exposures led to the production and application of biomarkers of exposure and effect and to our awareness of genotoxicity and its chronic manifestations, such as neoplasms, in wild fishes. To gain acceptance of these findings in the greater environmental toxicology community, "validation of the model" versus other, better-established often rodent models, was necessary and became a major focus. Resultant biomarkers were applied to heavily contaminated and reference field sites as part of effects assessment and with investigations following large-scale disasters such as oil spills or industrial accidents. Over the past 15 years, in the laboratory, small aquarium fish models such as medaka (Oryzias latipes), zebrafish (Danio rerio), platyfish (Xiphophorus species), fathead minnow (Pimephales promelas), and sheepshead minnow (Cyprinodon variegatus) were increasingly used establishing mechanisms of toxicants. Today, the same organisms provide reliable information at higher levels of biological organization relevant to ecotoxicology. We review studies resolving mechanisms of toxicity and discuss ways to address biocomplexity, mixtures of contaminants, and the need to relate individual level responses to populations and communities.
authors.affiliation: Laboratory of Molecular Aquatic Toxicology, Division of Environmental Sciences and Policy, Nicholas School of the Environment and Earth Sciences, Duke University Durham, NC 277-8-0328, USA. dhinton@duke.edu
authors.forename: David E
authors.initials: DE
authors.lastname: Hinton
authors.forename: Seth W
authors.initials: SW
authors.lastname: Kullman
authors.forename: Ron C
authors.initials: RC
authors.lastname: Hardman
authors.forename: David C
authors.initials: DC
authors.lastname: Volz
authors.forename: Pei-Jen
authors.initials: PJ
authors.lastname: Chen
authors.forename: Michael
authors.initials: M
authors.lastname: Carney
authors.forename: David C
authors.initials: DC
authors.lastname: Bencic
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism*; D015197:Carcinogenicity Tests / Q000379:methods*; D004463:Ecology / Q000379:methods*; D052244:Endocrine Disruptors / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism*; D023421:Models, Animal; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D014116:Toxicology / Q000379:methods / Q000639:trends*
chemical_list: D052244:Endocrine Disruptors; D004785:Environmental Pollutants