PKC pathway associated with the expression of an A-type K+ channel induced by TGF-beta1 in rat vascular myofibroblasts.
Our previous study indicated that TGF-beta1 induced the expression of a transient outward K+ channel (A-type) during the phenotypic transformation of vascular fibroblasts to myofibroblasts. Here, we studied the relevant signal transduction pathway using whole cell recording and a quantitative RT-PCR technique. Results indicate that the protein kinase C (PKC) agonist phorbol-12-myristate-13-acetate (PMA, 1 microM) could mimic the effect of TGF-beta1 (20 ng/ml) on the expression of an A-type K+ channel and induced a similar A-type K+ current. Moreover, a PKC inhibitor, bisindolylmaleimide I (1 microM), could abrogate the effect of TGF-beta1 on K(V)4.2 expression. This result suggests that a PKC pathway may be involved in the expression of an A-type K+ channel induced by TGF-beta1 in rat vascular myofibroblasts.
Liao
Lei
L
Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
Zeng
Xi-Min
XM
Gao
Ping-Jin
PJ
Zhu
Ding-Liang
DL
Mei
Yan-Ai
YA
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D004553:Electric Conductivity; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D015870:Gene Expression; D008297:Male; D032342:Muscle Cells / Q000166:cytology; D018408:Patch-Clamp Techniques; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051672:Shal Potassium Channels; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
D024642:Potassium Channels, Voltage-Gated; D051672:Shal Potassium Channels; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D011493:Protein Kinase C