In vivo modification of adriamycin-induced apoptosis in L-5178Y lymphoma cell-bearing mice by (+)-alpha-tocopherol and superoxide dismutase.
Apoptosis was followed in L5178Y lymphoma cell-bearing mice at different times after intraperitoneal injections of adriamycin (ADM). Apoptosis was determined morphologically and confirmed by DNA laddering on electrophoresis. Apoptosis was observed 36h after injection of 5mg/kg ADM (apoptotic cell index 64.2+/-5.6 vs. 1.5+/-2.1 from the untreated group) and confirmed by DNA electrophoresis. However, when the animals were pretreated with (+)-alpha-tocopherol acid succinate or superoxide dismutase before ADM administration apoptotic index significantly diminished (P<0.05) and the DNA electrophoresis did not show fragmentations. We conclude that in ADM-treated mice, tumour cell death occurs in two ways: first by necrosis, then later by apoptosis. These observations are likely to be associated with or caused by the generation of reactive oxygen species.
Bravo-Cuellar
A
A
CUALTOS, Universidad de Guadalajara, Guadalajara, Jal. México. abravoc@prodigy.net.mx
Gómez-Contreras
P C
PC
Lerma-Díaz
J M
JM
Hernández-Flores
G
G
Domínguez-Rodríguez
J R
JR
Ortíz-Lazareno
P
P
Cervantes-Munguia
R
R
Orbach-Arbouys
S
S
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004317:Doxorubicin / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008223:Lymphoma / Q000473:pathology* / Q000662:veterinary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009336:Necrosis; D017382:Reactive Oxygen Species / Q000009:adverse effects; D013482:Superoxide Dismutase / Q000378:metabolism*; D024505:Tocopherols; D014183:Transplantation, Heterologous; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology
D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D017382:Reactive Oxygen Species; D014810:Vitamin E; D004317:Doxorubicin; D013482:Superoxide Dismutase; D024505:Tocopherols