Analysis of the molecular variability of Grapevine leafroll-associated virus 1 reveals the presence of two distinct virus groups and their mixed occurrence in grapevines.
Genetic diversity of eight Grapevine leafroll-associated virus 1 (GLRaV-1) isolates recovered from grapevines in three distinct locations in the Czech Republic and Slovakia was characterised by restriction fragment length polymorphism (RFLP) analysis and by sequencing of cloned 540 bp fragment of the heat shock protein 70 (HSP70) gene. Comparison and phylogenetic analysis of obtained and previously available sequence data revealed the existence of two groups of GLRaV-l isolates, tentatively named A and E (genetic divergence between A and E group reached 13.9%). An RT-PCR detection method followed by simple restriction analysis was developed, showing the potential to differentiate GLRaV-1 isolates of these groups. Interestingly, a mixed infection of two GLRaV-1 groups in the same plant was frequently detected together with a high intra-group variability in some isolates.
Komínek
Petr
P
Research Institute of Crop Production, Drnovská 507, 161 06, Prague 6, Ruzyne, Czech Republic. kominek@vurv.cz
Glasa
Miroslav
M
Bryxiová
Marcela
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D029381:Closteroviridae / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D018153:Czech Republic; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D014644:Genetic Variation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D012150:Polymorphism, Restriction Fragment Length; D017386:Sequence Homology, Amino Acid; D018154:Slovakia; D014764:Viral Proteins / Q000235:genetics; D027843:Vitis / Q000821:virology*
D004279:DNA, Viral; D018840:HSP70 Heat-Shock Proteins; D014764:Viral Proteins