Lung development is not necessary for diaphragm development in mice.
OBJECTIVE
Congenital diaphragmatic hernia affects approximately 1 in every 2000 live births. The etiology of these diaphragmatic defects is unknown. Using mice with a targeted deletion of fibroblast growth factor 10 (FGF10), which display a complete lack of lung tissue, we have examined the relationship between lung hypoplasia and diaphragmatic development.


METHODS
The diaphragms of FGF10 null mice were examined at 2 embryonic time-points and compared with their heterozygous and wild-type littermates.


RESULTS
FGF10 null mice had phenotypically normal diaphragms when compared with wild-type littermates at both time-points studied.


CONCLUSIONS
Normal diaphragm development appears to occur independent of lung development in mice.
Arkovitz
Marc S
MS
Division of Pediatric Surgery, Children's Hospital of New York, New York, NY 10032-3784, USA. ma2161@columbia.edu
Hyatt
Brian A
BA
Shannon
John M
JM
D000818:Animals; D003964:Diaphragm / Q000196:embryology*; D051526:Fibroblast Growth Factor 10 / Q000235:genetics; D006548:Hernia, Diaphragmatic / Q000503:physiopathology*; D007150:Immunohistochemistry; D008168:Lung / Q000196:embryology* / Q000473:pathology; D051379:Mice; D010641:Phenotype
C496464:Fgf10 protein, mouse; D051526:Fibroblast Growth Factor 10