title: Activity of some aminoglycoside antibiotics against true fungi, Phytophthora and Pythium species.
abstract: OBJECTIVE
  To investigate the in vitro antifungal and antioomycete activities of some aminoglycosides against true fungi and Phytophthora and Pythium species and to evaluate the potential of the antibiotics against Phytophthora late blight on plants.
  
  
  RESULTS
  Antifungal and antioomycete activities of aminoglycoside antibiotics (neomycin, paromomycin, ribostamycin and streptomycin) and a paromomycin-producing strain (Streptomyces sp. AMG-P1) against Phytophthora and Pythium species and 10 common fungi were measured in potato dextrose broth (PDB) and on seedlings in pots. Paromomycin was the most active against Phytophthora and Pythium species with a minimal inhibitory concentration of 1-10 microg ml(-1) in PDB, but displayed low to moderate activities towards other common fungi at the same concentration. Paromomycin also showed potent in vivo activity against red pepper and tomato late blight diseases with 80 and 99% control value, respectively, at 100 microg ml(-1). In addition, culture broth of Streptomyces sp. AMG-P1 as a paromomycin producer exhibited high in vivo activity against late blight at 500 microg freeze-dried weight per millilitre.
  
  
  CONCLUSIONS
  Among tested aminoglycoside antibiotics, paromomycin was the most active against oomycetes both in vitro and in vivo.
  
  
  CONCLUSIONS
  Data from this study show that aminoglycoside antibiotics have in vitro and in vivo activities against oomycetes, suggesting that Streptomyces sp. AMG-P1 may be used as a biocontrol agent against oomycete diseases.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, Korea. fungallee@yahoo.com
authors.forename: H B
authors.initials: HB
authors.lastname: Lee
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
authors.forename: J C
authors.initials: JC
authors.lastname: Kim
authors.forename: G J
authors.initials: GJ
authors.lastname: Choi
authors.forename: S-H
authors.initials: SH
authors.lastname: Park
authors.forename: C-J
authors.initials: CJ
authors.lastname: Kim
authors.forename: H S
authors.initials: HS
authors.lastname: Jung
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002212:Capsicum / Q000382:microbiology; D003470:Culture Media; D005658:Fungi / Q000187:drug effects*; D018551:Solanum lycopersicum / Q000382:microbiology; D009355:Neomycin / Q000494:pharmacology*; D010303:Paromomycin / Q000494:pharmacology; D010838:Phytophthora / Q000187:drug effects*; D010935:Plant Diseases / Q000209:etiology / Q000382:microbiology; D011775:Pythium / Q000187:drug effects*; D012271:Ribostamycin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D012271:Ribostamycin; D010303:Paromomycin; D009355:Neomycin; D013307:Streptomycin