title: Mode of collection does not influence haematopoietic content of umbilical cord blood units from caesarean deliveries.
abstract: BACKGROUND
  Collection strategy is the first step for collecting good quality cord blood units. There are two main different techniques for collecting cord blood from the umbilical vein: in the delivery room while the placenta is still in the utero by midwifes and obstetricians, or in an adjacent room after placental delivery by cord blood bank trained personal. Our aim was to evaluate the benefits and disadvantages between the two different cord blood collection strategies in caesarean deliveries.
  
  
  METHODS
  We retrospectively analysed data of cord blood units collected from caesarean deliveries for a 3-year period. Caesarean section was performed with a low uterine transversal incision in all patients according to common obstetrical practice. Cord blood collection was performed before or after placental delivery.
  
  
  RESULTS
  Obstetrical and umbilical cord blood data was obtained from 253 caesarean deliveries. No statistically significant difference was observed for obstetrical variables or cord blood variables except for Hct and platelets.
  
  
  CONCLUSIONS
  We conclude both methods produce comparable TNC, CD34 and CFU counts of cord blood units collected from caesarean sections. Before placental delivery collection avoids the financial investment that generates the presence of cord blood banking personal in the maternity ward.
authors.affiliation: Umbilical Cord Blood Bank, Valencia, Spain. solves_pil@gva.es
authors.forename: Pilar
authors.initials: P
authors.lastname: Solves
authors.forename: Manuel
authors.initials: M
authors.lastname: Fillol
authors.forename: Mara
authors.initials: M
authors.lastname: López
authors.forename: Alfredo
authors.initials: A
authors.lastname: Perales
authors.forename: Fernando
authors.initials: F
authors.lastname: Bonilla-Musoles
authors.forename: Vicente
authors.initials: V
authors.lastname: Mirabet
authors.forename: M Angeles
authors.initials: MA
authors.lastname: Soler
authors.forename: Roberto J
authors.initials: RJ
authors.lastname: Roig
mesh_terms: D018952:Antigens, CD34 / Q000097:blood; D001800:Blood Specimen Collection / Q000379:methods*; D002452:Cell Count / Q000379:methods; D002585:Cesarean Section; D015925:Cryopreservation; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods; D005865:Gestational Age; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy; D012189:Retrospective Studies; D014470:Umbilical Cord; D014471:Umbilical Veins
chemical_list: D018952:Antigens, CD34