Diurnal H-reflex variation in mice.
Mice exhibit diurnal variation in complex motor behaviors, but little is known about diurnal variation in simple spinally mediated functions. This study describes diurnal variation in the H-reflex (HR), a wholly spinal and largely monosynaptic reflex. Six mice were implanted with tibial nerve cuff electrodes and electrodes in the soleus and gastrocnemius muscles, for recording of ongoing and nerve-evoked electromyographic activity (EMG). Stimulation and recording were under computer control 24 h/day. During a 10-day recording period, HR amplitude varied throughout the day, usually being larger in the dark than in the light. This diurnal HR variation could not be attributed solely to differences in the net ongoing level of descending and segmental excitation to the spinal cord or stimulus intensity. HRs were larger in the dark than in the light even after restricting the evoked responses to subsets of trials having similar ongoing EMG and M-responses. The diurnal variation in the HR was out of phase with that reported previously for rats, but was in phase with that observed in monkeys. These data, supported by those in other species, suggest that the supraspinal control of the excitability of the HR pathway varies throughout the day in a species-specific pattern. This variation should be taken into account in experimental and clinical studies of spinal reflexes recorded at different times of day.
Carp
Jonathan S
JS
Laboratory of Nervous System Disorders, Wadsworth Center, New York State Department of Health, State University of New York, Albany, NY 12201-0509, USA. carpj@wadsworth.org
Tennissen
Ann M
AM
Chen
Xiang Yang
XY
Wolpaw
Jonathan R
JR
D000818:Animals; D001921:Brain / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology*; D004525:Efferent Pathways / Q000502:physiology; D004558:Electric Stimulation; D004576:Electromyography; D006181:H-Reflex / Q000502:physiology*; D008297:Male; D051379:Mice; D009046:Motor Neurons / Q000502:physiology; D009119:Muscle Contraction / Q000502:physiology; D018482:Muscle, Skeletal / Q000294:innervation / Q000502:physiology; D009431:Neural Conduction / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D013045:Species Specificity; D013116:Spinal Cord / Q000502:physiology*; D013979:Tibial Nerve / Q000502:physiology*