title: Genome-wide analysis of retroviral DNA integration.
abstract: Retroviral vectors are often used to introduce therapeutic sequences into patients' cells. In recent years, gene therapy with retroviral vectors has had impressive therapeutic successes, but has also resulted in three cases of leukaemia caused by insertional mutagenesis, which has focused attention on the molecular determinants of retroviral-integration target-site selection. Here, we review retroviral DNA integration, with emphasis on recent genome-wide studies of targeting and on the status of efforts to modulate target-site selection.
authors.affiliation: University of Pennsylvania School of Medicine, Department of Microbiology, 3610 Hamilton Walk, Philadelphia, Pennsylvania 19104-6076, USA. bushman@mail.med.upenn.edu
authors.forename: Frederic
authors.initials: F
authors.lastname: Bushman
authors.forename: Mary
authors.initials: M
authors.lastname: Lewinski
authors.forename: Angela
authors.initials: A
authors.lastname: Ciuffi
authors.forename: Stephen
authors.initials: S
authors.lastname: Barr
authors.forename: Jeremy
authors.initials: J
authors.lastname: Leipzig
authors.forename: Sridhar
authors.initials: S
authors.lastname: Hannenhalli
authors.forename: Christian
authors.initials: C
authors.lastname: Hoffmann
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics; D011533:Proviruses / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D013045:Species Specificity; D016662:Virus Integration
chemical_list: D018076:DNA, Complementary