Evaluating human embryonic germ cells: concord and conflict as pluripotent stem cells.
The realization of cell replacement therapy derived from human pluripotent stem cells requires full knowledge of the starting cell types as well as their differentiated progeny. Alongside embryonic stem cells, embryonic germ cells (EGCs) are an alternative source of pluripotent stem cell. Since 1998, four groups have described the derivation of human EGCs. This review analyzes the progress on derivation, culture, and differentiation, drawing comparison with other pluripotent stem cell populations.
Turnpenny
Lee
L
Early Human Development and Stem Cells Group, University of Southampton, Duthie Building (M.P. 808), Southampton General Hospital, Tremona Road, Southampton SO16 6YD, United Kingdom.
Spalluto
Cosma M
CM
Perrett
Rebecca M
RM
O'Shea
Marie
M
Hanley
Karen Piper
KP
Cameron
Iain T
IT
Wilson
David I
DI
Hanley
Neil A
NA
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000737:chemistry / Q000502:physiology*; D006133:Growth Substances / Q000502:physiology; D006801:Humans; D051379:Mice; D039904:Pluripotent Stem Cells / Q000502:physiology*
D003470:Culture Media; D006133:Growth Substances