title: Genome-wide transcriptional variation within and between steady states for continuous growth of the hyperthermophile Thermotoga Maritima.
abstract: Maltose-limited, continuous growth of the hyperthermophile Thermotoga maritima at different temperatures and dilution rates (80 degrees C/0.25 h(-1), 80 degrees C/0.17 h(-1), and 85 degrees C/0.25 h(-1)) showed that transcriptome-wide variation in gene expression within mechanical steady states was minimal compared to that between steady states, supporting the efficacy of chemostat-based approaches for functional genomics studies.
authors.affiliation: Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
authors.forename: Keith R
authors.initials: KR
authors.lastname: Shockley
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Scott
authors.forename: Marybeth A
authors.initials: MA
authors.lastname: Pysz
authors.forename: Shannon B
authors.initials: SB
authors.lastname: Conners
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Johnson
authors.forename: Clemente I
authors.initials: CI
authors.lastname: Montero
authors.forename: Russell D
authors.initials: RD
authors.lastname: Wolfinger
authors.forename: Robert M
authors.initials: RM
authors.lastname: Kelly
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D006358:Hot Temperature; D008320:Maltose / Q000378:metabolism; D020543:Proteome; D020124:Thermotoga maritima / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D020543:Proteome; D008320:Maltose