Trigeminal P2X3 receptor expression differs from dorsal root ganglion and is modulated by deep tissue inflammation.
The distribution and modulation of the P2X(3) receptor was studied in trigeminal ganglion neurons to provide insight into the role of ATP in craniofacial sensory mechanisms. Binding to the d-galactose specific lectin IB4 was found in 73% of P2X(3)-positive neurons while only 16% of IB4 neurons expressed P2X(3). Neurons expressing P2X(3) alone were significantly larger than IB4-or IB4/P2X(3)-positive neurons. Investigation of target-specificity revealed that 22% of trigeminal ganglion muscle afferent neurons were positive for P2X(3) versus 16% of cutaneous afferent neurons. Muscle P2X(3) afferents were significantly smaller than the overall muscle afferent population while P2X(3) cutaneous afferent neurons were not. Presumptive heteromeric (P2X(2/3)) muscle afferent neurons were also identified and comprised 77% of the P2X(3) muscle afferent population. Muscle afferent neurons co-expressed P2X(3) with either calcitonin gene-related peptide (15%) or substance P (4%). The number of P2X(3)-positive muscle afferent neurons significantly increased one and four days following complete Freund's adjuvant-induced masseter muscle inflammation, but significantly decreased after 12 days. These results indicate that within trigeminal ganglia: (1) the P2X(3) receptor is expressed in both small and medium-sized neurons; (2) the P2X(3) receptor is not exclusively expressed in IB4 neurons; (3) P2X(3) is co-expressed with neuropeptides; (4) differences in the proportion of cutaneous versus muscle P2X(3) afferents are not apparent. Trigeminal P2X(3) neurons therefore differ markedly from dorsal root ganglion P2X(3) afferents. This study also shows that deep tissue inflammation modulates expression of the P2X(3) receptor and thus may warrant exploration as a target for therapeutic intervention.
Ambalavanar
Ranjinidevi
R
Department of Biomedical Sciences and Program in Neuroscience, University of Maryland, 666 West Baltimore Street, Baltimore, MD 21201, USA Department of Oral Anatomy and Neurobiology, Osaka University, Osaka 565-0871, Japan.
Moritani
Masayuki
M
Dessem
Dean
D
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D048429:Cell Size; D003911:Dextrans / Q000378:metabolism; D005620:Freund's Adjuvant; D005727:Ganglia, Spinal / Q000166:cytology*; D005786:Gene Expression Regulation / Q000235:genetics*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009220:Myositis / Q000139:chemically induced / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism*; D011043:Pokeweed Mitogens / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D058477:Receptors, Purinergic P2X3; D012235:Rhodamines / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000294:innervation / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000166:cytology*
D003911:Dextrans; D011043:Pokeweed Mitogens; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; D012235:Rhodamines; C105830:lectin B, Phytolacca americana; C051311:rhodamine dextran; D005620:Freund's Adjuvant; D015740:Calcitonin Gene-Related Peptide