Enzyme immunoassay for the determination of carbaryl residues in agricultural products.
Carbaryl is one of the most important N-methyl carbamate insecticides used all over the world. To determine carbaryl residues in agricultural products (apple, pear, Chinese cabbage, rice and barley), a competitive enzyme-linked immunosorbent assay (ELISA) method was employed, and a simple and efficient extraction method for the rapid detection of carbaryl in agricultural products was developed with recoveries greater than 90%. After liquid extraction, sample extracts diluted with buffer were analysed by ELISA directly. Matrix effects can be avoided when a 1:20 dilution of apple, pear, rice and barley samples and a 1:50 dilution of Chinese cabbage samples with 0.5% fish skin gelation in phosphate-buffered saline containing 5% methanol. Sample extracts were analysed by ELISA and HPLC, and results obtained by two methods correlated well; the correlation was greater than 0.9.
Wang
Shuo
S
Tianjin Key Laboratory of food Nutrition and Safety, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, China. s.wang@tust.edu.cn
Yu
Chundi
C
Wang
Junping
J
D001937:Brassica / Q000737:chemistry; D012721:Carbaryl / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D001467:Hordeum / Q000737:chemistry; D007306:Insecticides / Q000032:analysis*; D027845:Malus / Q000737:chemistry; D012275:Oryza / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis*; D031989:Pyrus / Q000737:chemistry
D007306:Insecticides; D010573:Pesticide Residues; D012721:Carbaryl