title: Generation and characterization of reduced virulence Fusarium oxysporum f. sp. lycopersici mutants through plasmid-vector insertion.
abstract: Pathogenicity-impaired mutants, B02 and H15, of Fusarium oxysporum f. sp. lycorpersici (FOL) were obtained using restriction enzyme-mediated integration. Disease severities of Fusarium wilt caused by these mutants were significantly reduced, and their disease development rates were correlated with their colonization rates in tomato vessels. Both B02 and H15 produced significantly smaller amounts of extracellular proteins as well as fusaric acid than the wild-type. Southern blot analyses suggested that B02 and H15 likely contain a single and three copies of transformation vector, respectively. These mutants may thus be useful in isolating genes involved in pathogenicity of FOL.
authors.affiliation: Graduate School of Science and Technology, Chiba University, Yayoicho-1-33, Inage, Chiba-city, Chiba, 263-8522, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Morita
authors.forename: Soichiro
authors.initials: S
authors.lastname: Kimura
authors.forename: Masanori
authors.initials: M
authors.lastname: Saito
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Shinoyama
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Usami
authors.forename: Yoshimiki
authors.initials: Y
authors.lastname: Amemiya
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shishido
mesh_terms: D004262:DNA Restriction Enzymes; D005670:Fusarium / Q000235:genetics* / Q000472:pathogenicity; D018551:Solanum lycopersicum / Q000382:microbiology*; D016254:Mutagenesis, Insertional; D009181:Mycoses / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D010957:Plasmids; D014774:Virulence / Q000235:genetics
chemical_list: D004262:DNA Restriction Enzymes