title: Endoplasmic reticulum stress response of Bombyx mori calreticulin.
abstract: We isolated a calreticulin cDNA from the silkworm, Bombyx mori. The cDNA encodes 398 amino acid residues of B. mori calreticulin, with an endoplasmic reticulum retentional HDEL motif at its C-terminus and a predicted molecular mass of 45,801 Da. The B. mori calreticulin shows high protein homology with calreticulin from G. mellonella (88%), A. aegypti (71%), D. melanogaster (69%) and H. sapiens (63%). The highest level of mRNA expression of B. mori calreticulin was exhibited in the fat body of this insect. Although expression of B. mori calreticulin was affected by disturbances in intracellular calcium levels, other ER stress conditions such as inhibition of intracellular protein transport, reduction of disulfide formation, glycosylation inhibition, heat shock and oxidative stress did not disrupt induction of B. mori calreticulin.
authors.affiliation: Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Suwon, 441-744, Korea.
authors.forename: Tae Won
authors.initials: TW
authors.lastname: Goo
authors.forename: Soojung
authors.initials: S
authors.lastname: Park
authors.forename: Byung Rae
authors.initials: BR
authors.lastname: Jin
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Yun
authors.forename: Iksoo
authors.initials: I
authors.lastname: Kim
authors.forename: Si-Kab
authors.initials: SK
authors.lastname: Nho
authors.forename: Seok-Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: O-Yu
authors.initials: OY
authors.lastname: Kwon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism; D037282:Calreticulin / Q000096:biosynthesis / Q000235:genetics*; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000502:physiology*; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D037282:Calreticulin; D019476:Insect Proteins; D012333:RNA, Messenger