Evaluation of a capillary immunoassay system for detection of Salmonella typhimurium in poultry products.
A capillary immunoassay system was constructed and optimized for detection of Salmonella Typhimurium. The system consisted of a capillary bioseparator-bioreactor and a flow-injection electrochemical detector. Three methods were compared for immobilizing antibodies on the inner surface of silica capillary columns; these methods were based on the use of a homobifunctional cross-linker glutaraldehyde, a heterobifunctional cross-linker N-succinimidyl-4-maleimidobutyrate, and biotin-streptavidin chemistry, respectively. The glutaraldehyde method gave the best reproducibility with a relative standard deviation of 1 to 6% for detection of Salmonella Typhimurium. The optimized immunoassay system could detect Salmonella Typhimurium in chicken breast and ground turkey meats with a detection limit of 2.4 x 10(3) and 2.4 x 10(4) CFU/ml, respectively. The total detection time was less than 2.5 h without any preenrichment. When stored at 4 degrees C, the immunocolumns could retain their activities for at least 3 months.
Kim
Byungchul
B
Department of Food Science, University of Arkansas, Fayetteville, Arkansas 72701, USA.
Su
Xiao-Li
XL
Li
Yanbin
Y
D000907:Antibodies, Bacterial / Q000032:analysis*; D019149:Bioreactors / Q000382:microbiology; D015169:Colony Count, Microbial; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D007124:Immunoenzyme Techniques / Q000379:methods*; D011202:Poultry Products / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000276:immunology* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013997:Time Factors
D000907:Antibodies, Bacterial