Genome-wide transcriptional variation within and between steady states for continuous growth of the hyperthermophile Thermotoga Maritima.
Maltose-limited, continuous growth of the hyperthermophile Thermotoga maritima at different temperatures and dilution rates (80 degrees C/0.25 h(-1), 80 degrees C/0.17 h(-1), and 85 degrees C/0.25 h(-1)) showed that transcriptome-wide variation in gene expression within mechanical steady states was minimal compared to that between steady states, supporting the efficacy of chemostat-based approaches for functional genomics studies.
Shockley
Keith R
KR
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
Scott
Kevin L
KL
Pysz
Marybeth A
MA
Conners
Shannon B
SB
Johnson
Matthew R
MR
Montero
Clemente I
CI
Wolfinger
Russell D
RD
Kelly
Robert M
RM
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D006358:Hot Temperature; D008320:Maltose / Q000378:metabolism; D020543:Proteome; D020124:Thermotoga maritima / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D003470:Culture Media; D020543:Proteome; D008320:Maltose