Molecular cloning and characterization of monoamine oxidase A and B genes in pig.
Monoamine oxidase (MAO) is a key enzyme in the degradation of monoamine neurotransmitters, which are physiologically and pathologically important for animals and humans. MAO exists in two forms, coded by separate genes. Here we cloned and sequenced full length cDNAs of MAO-A and MAO-B in pig. The putative proteins include 527 and 520 amino acids, respectively. The sequences alignment and homology analysis showed that MAO-A and MAO-B are conserved among mammals. The amino acid residues and domains, which have been demonstrated to be important for MAO-A and MAO-B binding of ligands, substrates and inhibitors, were conserved in the pig.
Bai
Chunyan
C
Department of Animal Science, Agriculture and Biology College, Shanghai Jiao Tong University, P.R. China.
Meng
He
H
Zhao
Wei
W
Pan
Yuchun
Y
D000588:Amines / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D008995:Monoamine Oxidase / Q000235:genetics*; D018377:Neurotransmitter Agents / Q000737:chemistry; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012313:RNA / Q000737:chemistry; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014018:Tissue Distribution
D000588:Amines; D018076:DNA, Complementary; D018377:Neurotransmitter Agents; D012313:RNA; D008995:Monoamine Oxidase