Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae.
Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3Delta1. Leaky expression was eliminated using 3-aminotriazole and gene conversion was eliminated by using the Schizosaccharomyces pombe his5+ gene, resulting in a 5- to 10-fold improvement in the efficiency of SGA.
Daniel
Jewel A
JA
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210-2339, USA.
Yoo
Jiyoun
J
Bettinger
Blaine T
BT
Amberg
David C
DC
Burke
Daniel J
DJ
D000640:Amitrole / Q000378:metabolism*; D015825:Chromosomes, Fungal; D005656:Fungal Proteins / Q000235:genetics*; D005785:Gene Conversion; D016681:Genome, Fungal; D006639:Histidine / Q000235:genetics*; D046228:Microarray Analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*
D005656:Fungal Proteins; D006639:Histidine; D000640:Amitrole