Sufficient penetration of peracetic acid into drilled human femoral heads.
Chemical sterilisation methods for musculoskeletal transplants have the problem of penetration into all tissue strata. The present study examined if a peracetic acid/ethanol solution penetrated to a sufficient extent into specifically prepared femoral heads. To this effect, 10 femoral heads have been provided with drillings (diameter 2 mm, depth 10 mm) at a distance of 15 mm (series B) and placed in a diffusion chamber with sterilisation solution. From an additional central drilling at the femoral neck junction, the sample drawing was made after 30 min each over a period of 4 h for the iodometric determination of peracetic acid (PAA) concentration. Ten femoral heads, which did contain only the central drilling, served as controls (series A). In 9 of the examined femoral heads of series A the defined minimum concentration of PAA of 0.2% (inactivation of bacteria, spores, fungi) has been clearly exceeded over the complete period of measurement. About 0.8% PAA (inactivation of viruses) was achieved within 4 h only with six femoral heads. Nine out of the 10 examined femoral heads in series B show a clearly improved penetration behaviour which was expressed in smaller standard deviations, a faster increase in concentration, as well as in higher starting and final concentrations (approx. 0.9%) of PAA. Previous drying in air leads to a faster penetration into the centre of the bone. Standardised drilling of de-cartilaged femoral heads creates favourable conditions for the penetration of the PAA sterilisation solution into the whole tissue and guarantees a sufficient inactivation of microorganisms.
Brosig
H
H
Institute of Pharmacy, Free University Berlin, Germany.
Jacker
H-J
HJ
Borchert
H-H
HH
Kalus
U
U
Dörner
T
T
von Versen
R
R
Pruss
A
A
D016025:Bone Transplantation; D005270:Femur Head / Q000187:drug effects* / Q000637:transplantation; D006801:Humans; D010463:Peracetic Acid / Q000494:pharmacology*; D013242:Sterilization; D013997:Time Factors; D014015:Tissue Banks; D014184:Transplantation, Homologous
D010463:Peracetic Acid