Quaternary ammonium polysaccharides for gene delivery.
Cationic polysaccharides were synthesized by conjugation of various monoquaternary (MQ) ammonium oligoamines to oxidized dextran by reductive amination and tested for gene transfection. Polycations of dextran grafted with MQ ammonium oligoamines of two to four amino groups were investigated for their ability to cause pCMV-GFP encoding for green fluorescence protein and beta-Gal encoding for beta-galactosidase protein transfection on EPC and HEK-293 cell lines. These polycations were expected to strongly complex DNA due to increased surface cationic charge of the carrier, which may result in a higher transfection yield. However, the transfection yields were much lower compared to the parent vector, dextran-spermine conjugate, which was highly effective both in vitro and in vivo.
Yudovin-Farber
Ira
I
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, P.O.B. 12065 Jerusalem 91120, Israel.
Yanay
Chava
C
Azzam
Tony
T
Linial
Michal
M
Domb
Abraham J
AJ
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D014162:Transfection / Q000295:instrumentation* / Q000379:methods*
D011134:Polysaccharides; D000644:Quaternary Ammonium Compounds; D004247:DNA