Efficiency and limits of the Serial Analysis of Gene Expression (SAGE) method: discussions based on first results in bovine trypanotolerance.
Post genomic biotechnologies, such as transcriptome analysis, are now efficient enough to characterize the full complement of genes involved in the expression of specific biological functions. One of them is the Serial Analysis of Gene Expression (SAGE) technique. SAGE involves the construction of transcript libraries for a quantitative analysis of the entire set of genes expressed or inactivated at particular stages of cellular activation. Bioinformatic comparisons in hosts and pathogens genomic databases allow the identification of several up- and down-regulated genes, ESTs and unknown transcripts directly involved in the host-pathogen immunological interaction mechanisms. Based on the first results obtained during an experimental Trypanosoma congolense infection in trypanotolerant cattle, the efficiency and limits of such a technique, from the data acquisition level to the data analysis level, is discussed in this analysis.
Maillard
Jean-Charles
JC
Cirad-Prise, c/o NIAH, Thuy Phuong, Tu Liem, Hanoi, Vietnam. maillard@fpt.vn
Berthier
David
D
Thevenon
Sophie
S
Piquemal
David
D
Chantal
Isabelle
I
Marti
Jacques
J
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics* / Q000276:immunology; D019295:Computational Biology; D004247:DNA / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods / Q000706:statistics & numerical data / Q000662:veterinary*; D023281:Genomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014348:Trypanosoma congolense; D014353:Trypanosomiasis, African / Q000235:genetics / Q000276:immunology / Q000662:veterinary*
D012333:RNA, Messenger; D004247:DNA