Amla (Emblica officinalis Gaertn.) extracts reduce oxidative stress in streptozotocin-induced diabetic rats.
The antioxidant properties of amla extracts and their effects on the oxidative stress in streptozotocin-induced diabetes were examined in rats. Amla in the form of either the commercial enzymatic extract SunAmla (Taiyo Kagaku Co. Ltd., Yokkaichi, Japan) (20 or 40 mg/kg of body weight/day) or a polyphenol-rich fraction of ethyl acetate extract (10 or 20 mg/kg of body weight/day) was given orally for 20 days to the streptozotocin-induced diabetic rats. Amla extracts showed strong free radical scavenging activity. Amla also showed strong inhibition of the production of advanced glycosylated end products. The oral administration of amla extracts to the diabetic rats slightly improved body weight gain and also significantly alleviated various oxidative stress indices of the serum of the diabetic rats. The elevated serum levels of 5-hydroxymethylfurfural, which is a glycosylated protein that is an indicator of oxidative stress, were significantly reduced dose-dependently in the diabetic rats fed amla. Similarly, the serum level of creatinine, yet another oxidative stress parameter, was also reduced. Furthermore, thiobarbituric acid-reactive substances levels were significantly reduced with amla, indicating a reduction in lipid peroxidation. In addition, the decreased albumin levels in the diabetic rats were significantly improved with amla. Amla also significantly improved the serum adiponectin levels. These results form the scientific basis supporting the efficacy of amla for relieving the oxidative stress and improving glucose metabolism in diabetes.
Rao
T P
TP
Bio-nutrition Division, Taiyo Kagaku Co. Ltd., Yokkaichi, Mie, Japan. tprao@taiyokagaku.co.jp
Sakaguchi
N
N
Juneja
L R
LR
Wada
E
E
Yokozawa
T
T
D052242:Adiponectin; D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis; D001713:Biphenyl Compounds; D001835:Body Weight; D003404:Creatinine / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D004326:Drinking; D004435:Eating; D005419:Flavonoids / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors; D006024:Glycopyrrolate; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood; D008297:Male; D009929:Organ Size; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000032:analysis; D029842:Phyllanthus emblica / Q000737:chemistry*; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D059808:Polyphenols; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000032:analysis; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D052242:Adiponectin; D001713:Biphenyl Compounds; D005419:Flavonoids; D017127:Glycation End Products, Advanced; D036341:Intercellular Signaling Peptides and Proteins; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D012709:Serum Albumin; D017392:Thiobarbituric Acid Reactive Substances; D003404:Creatinine; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid; D006024:Glycopyrrolate