title: Inactivation of the BSE agent by the heat and pressure process for manufacturing gelatine.
abstract: Dietary exposure to the bovine spongiform encephalopathy (BSE) agent is the probable cause of variant Creutzfeldt-Jakob disease in people. The industrial manufacturing process for the production of gelatine and colloidal protein by the heat and pressure process was downscaled accurately and its capacity to remove or inactivate bse infectivity was investigated. Gelatine was made from bones experimentally contaminated with mouse brain infected with the 301V strain of mouse-passaged bse agent in which the infective titre was 10(8.7) ID50/g. No infectivity was detected in the extracted protein (> or =10(0.45) ID50/g), and the calculated clearance factor was 10(6.5) ID50 or more.
authors.affiliation: Delft Gelatin, 4731 wh Oudenbosch, The Netherlands.
authors.forename: A H
authors.initials: AH
authors.lastname: Grobben
authors.forename: P J
authors.initials: PJ
authors.lastname: Steele
authors.forename: R A
authors.initials: RA
authors.lastname: Somerville
authors.forename: D M
authors.initials: DM
authors.lastname: Taylor
authors.forename: B E C
authors.initials: BE
authors.lastname: Schreuder
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D007562:Creutzfeldt-Jakob Syndrome / Q000517:prevention & control; D016643:Encephalopathy, Bovine Spongiform / Q000635:transmission*; D005780:Gelatin / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D011312:Pressure; D011328:Prions / Q000472:pathogenicity*
chemical_list: D011328:Prions; D005780:Gelatin