title: Extracellular protease of Pseudomonas fluorescens CHA0, a biocontrol factor with activity against the root-knot nematode Meloidogyne incognita.
abstract: In Pseudomonas fluorescens CHA0, mutation of the GacA-controlled aprA gene (encoding the major extracellular protease) or the gacA regulatory gene resulted in reduced biocontrol activity against the root-knot nematode Meloidogyne incognita during tomato and soybean infection. Culture supernatants of strain CHA0 inhibited egg hatching and induced mortality of M. incognita juveniles more strongly than did supernatants of aprA and gacA mutants, suggesting that AprA protease contributes to biocontrol.
authors.affiliation: Soil Biology and Ecology Laboratory, Department of Botany, University of Karachi, Karachi-75270, Pakistan.
authors.forename: Imran Ali
authors.initials: IA
authors.lastname: Siddiqui
authors.forename: Dieter
authors.initials: D
authors.lastname: Haas
authors.forename: Stephan
authors.initials: S
authors.lastname: Heeb
mesh_terms: D000818:Animals; D000969:Antinematodal Agents; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020689:Exopeptidases / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D018551:Solanum lycopersicum / Q000469:parasitology; D008969:Molecular Sequence Data; D009154:Mutation; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000469:parasitology; D018517:Plant Roots / Q000469:parasitology*; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000254:growth & development*; D013025:Glycine max / Q000469:parasitology; D014431:Tylenchoidea / Q000254:growth & development* / Q000382:microbiology
chemical_list: D000969:Antinematodal Agents; D001426:Bacterial Proteins; C091100:GacA protein, Bacteria; C449646:AprA protein, Pseudomonas fluorescens; D020689:Exopeptidases