title: Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library.
abstract: A 10-mer overlapping peptide library has been synthesized for screening and identification of linear B-cell epitopes of severe acute respiratory syndrome associated coronavirus (SARS-CoV), which spanned the major structural proteins of SARS-CoV. One hundred and eleven candidate peptides were positive according to the result of PEPscan, which were assembled into 22 longer peptides. Five of these peptides showed high cross-immunoreactivities (approximately 66.7 to 90.5%) to SARS convalescent patients' sera from the severest epidemic regions of the China mainland. Most interestingly, S(471-503), a peptide located at the receptor binding domain (RBD) of SARS-CoV, could specifically block the binding between the RBD and angiotensin-converting enzyme 2, resulting in the inhibition of SARS-CoV entrance into host cells in vitro. The study demonstrated that S(471-503) peptide was a potential immunoantigen for the development of peptide-based vaccine or a candidate for further drug evaluation against the SARS-CoV virus-cell fusion.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, 1 Xian Nong Tan Street, Beijing 100050, P. R. China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Hu
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Richard Y
authors.initials: RY
authors.lastname: Kao
authors.forename: Binbin
authors.initials: B
authors.lastname: Kou
authors.forename: Zhanguo
authors.initials: Z
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Hao
authors.forename: Wayne H
authors.initials: WH
authors.lastname: Tsui
authors.forename: Anping
authors.initials: A
authors.lastname: Ni
authors.forename: Lianxian
authors.initials: L
authors.lastname: Cui
authors.forename: Baoxing
authors.initials: B
authors.lastname: Fan
authors.forename: Feng
authors.initials: F
authors.lastname: Guo
authors.forename: Shuan
authors.initials: S
authors.lastname: Rao
authors.forename: Chengyu
authors.initials: C
authors.lastname: Jiang
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Manji
authors.initials: M
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D020650:Combinatorial Chemistry Techniques; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D019151:Peptide Library; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry* / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; D019151:Peptide Library