title: Ionic liquid-reconstituted cellulose composites as solid support matrices for biocatalyst immobilization.
abstract: Preparation of cellulose-polyamine composite films and beads, which provide high loading of primary amines on the surface allowing direct one-step bioconjugation of active species, is reported using an ionic liquid (IL) dissolution and regeneration process. Films and bead architectures were prepared and used as immobilization supports for laccase as a model system demonstrating the applicability of this approach. Performance of these materials, compared to commercially available products, has been assessed using millimeter-sized beads of the composites and the lipase-catalyzed transesterification of ethyl butyrate.
authors.affiliation: Center for Green Manufacturing, Department of Chemistry, The University of Alabama, Tuscaloosa, Alabama 35487, USA.
authors.forename: Megan B
authors.initials: MB
authors.lastname: Turner
authors.forename: Scott K
authors.initials: SK
authors.lastname: Spear
authors.forename: John D
authors.initials: JD
authors.lastname: Holbrey
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Daly
authors.forename: Robin D
authors.initials: RD
authors.lastname: Rogers
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001965:Bromides / Q000737:chemistry; D000440:Butanols / Q000737:chemistry; D002087:Butyrates / Q000737:chemistry; D002384:Catalysis; D002482:Cellulose / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D004800:Enzymes, Immobilized; D006835:Hydrazones / Q000737:chemistry; D007477:Ions; D042845:Laccase / Q000737:chemistry; D008049:Lipase / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D010100:Oxygen / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D001965:Bromides; D000440:Butanols; D002087:Butyrates; D004800:Enzymes, Immobilized; D006835:Hydrazones; D007477:Ions; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D011073:Polyamines; D011108:Polymers; C009370:syringaldazine; D002482:Cellulose; D042845:Laccase; D008049:Lipase; D010100:Oxygen; C045572:ethyl butyrate