title: EROD activity and biliary fluorescence in Schroederichthys chilensis (Guichenot 1848): biomarkers of PAH exposure in coastal environments of the South Pacific Ocean.
abstract: Schroederichthys chilensis is a common shark that lives in Chilean coastal environments. In this work, the relationship between liver 7-ethoxyresorufin-O-deethylase dealkylation (EROD) activity and Fluorescent Aromatic Compounds (FAC) in bile of S. chilensis sampled in three bays with different degrees of pollution were performed including a reference area. Sixty individuals were collected, 20 for each site; (10 males and 10 females per site) livers and bile samples were obtained and immediately frozen. EROD activity and FAC were measured according to three standard methods. EROD activity and FAC were higher in polluted areas than in the reference area. Synchronous Fluorescence Spectra of the bile from the fish collected at the most polluted area showed a peak at 347nm representing a metabolite corresponding to 1-hydroxypyrene. The low EROD activity in the reference area is likely related to the low level of PAH in sediments. We propose that this species is a good indicator of exposure to FACs, since it presents a series of characteristics that make it suitable for monitoring PAH exposure in coastal zones.
authors.affiliation: Faculty of Sciences, Department of Environmental Chemistry, Universidad Católica de la Santísima Concepción, P.O. Box 297, Concepción, Chile. daniefuentes@udec.cl
authors.forename: Daniel
authors.initials: D
authors.lastname: Fuentes-Rios
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Orrego
authors.forename: Anny
authors.initials: A
authors.lastname: Rudolph
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Mendoza
authors.forename: Juan F
authors.initials: JF
authors.lastname: Gavilán
authors.forename: Ricardo
authors.initials: R
authors.lastname: Barra
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D005260:Female; D005453:Fluorescence; D008297:Male; D010140:Pacific Ocean; D011084:Polycyclic Aromatic Hydrocarbons / Q000506:poisoning*; D012754:Sharks / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000506:poisoning*
chemical_list: D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1