Sulfur dioxide derivative modulation of potassium channels in rat ventricular myocytes.
The effects of sulfur dioxide (SO2) derivatives (bisulfite and sulfite, 1:3 M/M) on voltage-dependent potassium current in isolated adult rat ventricular myocyte were investigated using the whole cell patch-clamp technique. SO2 derivatives (10 microM) increased transient outward potassium current (I(to)) and inward rectifier potassium current (I(K1)), but did not affect the steady-state outward potassium current (I(ss)). SO2 derivatives significantly shifted the steady-state activation curve of I(to) toward the more negative potential at the V(h) point, but shifted the inactivation curve to more positive potential. SO2 derivatives markedly shifted the curve of time-dependent recovery of I(to) from the steady-state inactivation to the left, and accelerated the recovery of I(to) from inactivation. In addition, SO2 derivatives also significantly change the inactivation time constants of I(to) with increasing fast time constant and decreasing slow time constant. These results indicated a possible correlation between the change of properties of potassium channel and SO2 inhalation toxicity, which might cause cardiac myocyte injury through increasing extracellular potassium via voltage-gated potassium channels.
Nie
Aifang
A
Institute of Environmental Medicine and Toxicology, Shanxi University, Taiyuan 030006, PR China. afnie@126.com
Meng
Ziqiang
Z
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D051668:Delayed Rectifier Potassium Channels; D006352:Heart Ventricles / Q000166:cytology; D015640:Ion Channel Gating / Q000187:drug effects; D017136:Ion Transport / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D013447:Sulfites / Q000494:pharmacology; D013458:Sulfur Dioxide / Q000633:toxicity*; D016276:Ventricular Function
D000393:Air Pollutants; D051668:Delayed Rectifier Potassium Channels; D013447:Sulfites; D013458:Sulfur Dioxide; C042345:hydrogen sulfite