The glutathione peroxidase homologous gene Gpxh in Chlamydomonas reinhardtii is upregulated by singlet oxygen produced in photosystem II.
The expression of the glutathione peroxidase homologous gene Gpxh, known to be specifically induced by the formation of singlet oxygen (1O2), was analyzed in cells of Chlamydomonas reinhardtii exposed to environmental conditions causing photoinhibition. Illumination with high light intensities, leading to an increased formation of 1O2 in photosystem II, continuously induced the expression of Gpxh in cell for at least 2 h. Phenolic herbicides like dinoterb, raise the rate of 1O2 formation by increasing the probability of charge recombination in photosystem II via the formation of the primary radical pair and thereby 3P680 formation (Fufezan C et al. 2002, FEBS Letters 532, 407-410). In the presence of dinoterb the light-induced loss of the D1 protein in C. reinhardtii was increased and the high light-induced Gpxh expression was further stimulated. DCMU, a urea-type herbicide, causing reduced 1O2 generation in photosystem II, protected the D1 protein slightly against degradation and downregulated the expression of the Gpxh gene compared to untreated cells exposed to high light intensities. This indicates that the Gpxh expression is induced by 1O2 under environment conditions causing photoinhibition.
Fischer
Beat B
BB
Department of Environmental Toxicology, Swiss Federal Institute for Environmental Science and Technology (EAWAG), Uberlandstr. 133, Dubendorf 8600, Switzerland.
Eggen
Rik I L
RI
Trebst
Achim
A
Krieger-Liszkay
Anja
A
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005786:Gene Expression Regulation; D005979:Glutathione Peroxidase / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology; D008027:Light; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D026082:Singlet Oxygen / Q000378:metabolism*; D013113:Spin Labels; D019097:Spin Trapping; D018724:Spinacia oleracea / Q000378:metabolism / Q000648:ultrastructure; D020524:Thylakoids / Q000378:metabolism; D015854:Up-Regulation
D006540:Herbicides; D045332:Photosystem II Protein Complex; D015800:Protozoan Proteins; D013113:Spin Labels; D002734:Chlorophyll; D026082:Singlet Oxygen; C064041:chlorophyll c; D005979:Glutathione Peroxidase