title: An integrated microfluidic device for influenza and other genetic analyses.
abstract: An integrated microfluidic device capable of performing a variety of genetic assays has been developed as a step towards building systems for widespread dissemination. The device integrates fluidic and thermal components such as heaters, temperature sensors, and addressable valves to control two nanoliter reactors in series followed by an electrophoretic separation. This combination of components is suitable for a variety of genetic analyses. As an example, we have successfully identified sequence-specific hemagglutinin A subtype for the A/LA/1/87 strain of influenza virus. The device uses a compact design and mass production technologies, making it an attractive platform for a variety of widely disseminated applications.
authors.affiliation: Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: R
authors.initials: R
authors.lastname: Pal
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.forename: R
authors.initials: R
authors.lastname: Lin
authors.forename: B N
authors.initials: BN
authors.lastname: Johnson
authors.forename: N
authors.initials: N
authors.lastname: Srivastava
authors.forename: S Z
authors.initials: SZ
authors.lastname: Razzacki
authors.forename: K J
authors.initials: KJ
authors.lastname: Chomistek
authors.forename: D C
authors.initials: DC
authors.lastname: Heldsinger
authors.forename: R M
authors.initials: RM
authors.lastname: Haque
authors.forename: V M
authors.initials: VM
authors.lastname: Ugaz
authors.forename: P K
authors.initials: PK
authors.lastname: Thwar
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: K
authors.initials: K
authors.lastname: Alfano
authors.forename: M B
authors.initials: MB
authors.lastname: Yim
authors.forename: M
authors.initials: M
authors.lastname: Krishnan
authors.forename: A O
authors.initials: AO
authors.lastname: Fuller
authors.forename: R G
authors.initials: RG
authors.lastname: Larson
authors.forename: D T
authors.initials: DT
authors.lastname: Burke
authors.forename: M A
authors.initials: MA
authors.lastname: Burns
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000378:metabolism; D004586:Electrophoresis; D005898:Glass; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006358:Hot Temperature; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007251:Influenza, Human / Q000235:genetics*; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D044085:Microfluidics; D008904:Miniaturization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012825:Silicon / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; C483468:hemagglutinin, human influenza A virus; D012825:Silicon