Specific photodissociation of peptides with multi-stage mass spectrometry.
We report collision-induced dissociation (CID) and laser-induced dissociation (LID) performed at different wavelengths between 220 and 280 nm of the peptides leucine-enkephalin (protonated) and gramicidin A (sodiated). Hydrogen-atom losses and side-chain cleavages were observed in LID experiments. These losses depend on the laser wavelength and lead to the formation of radical ions. The fragmentations of these radicals, which are not observed in CID experiments, were investigated in multi-stage mass spectrometry experiments.
Tabarin
Thibault
T
Laboratoire de Spectrométrie Ionique et Moléculaire, UMR 5579 (Université Lyon I et CNRS), 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France.
Antoine
Rodolphe
R
Broyer
Michel
M
Dugourd
Philippe
P
D004743:Enkephalin, Leucine / Q000032:analysis* / Q000528:radiation effects*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006096:Gramicidin / Q000032:analysis* / Q000528:radiation effects*; D007834:Lasers; D010449:Peptide Mapping / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000032:analysis / Q000528:radiation effects; D010777:Photochemistry / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D014466:Ultraviolet Rays
D010455:Peptides; D006096:Gramicidin; D004743:Enkephalin, Leucine