Co-expression of IL-12 receptors along with CXCR3 and CD25 on activated peripheral blood T lymphocytes.
IL-12 receptors (IL-12R) play a critical role in maintaining IL-12 regulation of T helper-1 (Th1) type immune responses. We studied the expression of two IL-12R, beta1 and beta2 on peripheral blood mononuclear cells (PBMCs) from normal donors, stimulated with polyclonal activators in the presence or absence of exogenous rhIL-12. Unstimulated peripheral blood T lymphocytes (PBTs) expressed moderate levels of IL-12Rbeta1 and very low to undetectable levels of IL-12Rbeta2. Superantigens and anti-CD3+anti-CD28 induced higher expression of both IL-12R on PBTs than PHA-P stimulation. Exogenous rhIL-12 further enhanced the PHA-P or anti-CD3+anti-CD28 induced IL-12Rbeta2 expression. Only a fraction of mitogen activated IL-12Rbeta1+ or beta2+ T lymphocytes co-expressed CD25 (with further enhancement by exogenous rhIL-12), while a higher percentage of these cells were CXCR3+. The majority of superantigen or anti-CD3+anti-CD28-induced IL-12R+ PBTs were positive for both CD25 and CXCR3 markers. Our results indicated differential induction of IL-12R expression that correlated with up regulation of CD25 and CXCR3 expression on activated PBTs and provide a useful insight for monitoring these markers during treatment of Th1 type inflammatory diseases.
Reddy
Manjula
M
Department of Clinical Pharmacology and Experimental Medicine, Centocor Inc. 145 King of Prussia Road, Radnor, PA 19087-4517, USA. Mreddy@CNTUS.JNJ.com
Wong
Jackson
J
Davis
Cuc
C
Prabhakar
Uma
U
D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear; D008213:Lymphocyte Activation / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000378:metabolism*; D053707:Receptors, Interleukin-12; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D018089:Superantigens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism*
D018106:CD28 Antigens; D017252:CD3 Complex; C515187:CXCR3 protein, human; C508438:IL12RB1 protein, human; C508439:IL12RB2 protein, human; D010835:Phytohemagglutinins; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine; D018123:Receptors, Interleukin; D053707:Receptors, Interleukin-12; D015375:Receptors, Interleukin-2; D018089:Superantigens; C028784:phytohemagglutinin-P