title: Upregulation of the BRCA1 gene in human germ cells and in preimplantation embryos.
abstract: The quantification of BRCA1 messenger RNA molecules by a quantitative competitive one-step reverse transcriptase polymerase chain reaction method indicates that BRCA1 is upregulated both in human male and female germ cells and in preimplantation embryos. Because BRCA1 is involved in several pathways that participate in preserving intact chromosome and genome integrity, these data suggest that BRCA1 dysfunction might alter human embryogenesis or fertility.
authors.affiliation: Laboratoire de Biologie de la Reproduction et du Développement, Lyon, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Giscard d'Estaing
authors.forename: Delphine
authors.initials: D
authors.lastname: Perrin
authors.forename: Gilbert M
authors.initials: GM
authors.lastname: Lenoir
authors.forename: Jean François
authors.initials: JF
authors.lastname: Guérin
authors.forename: Robert
authors.initials: R
authors.lastname: Dante
mesh_terms: D001755:Blastocyst / Q000378:metabolism*; D005260:Female; D019398:Genes, BRCA1 / Q000502:physiology*; D005854:Germ Cells / Q000378:metabolism; D006801:Humans; D008297:Male; D009865:Oocytes / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology*