Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay.
A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for M. tuberculosis makes it more suitable method for MIC determination.
Kumar
Manoj
M
Biotechnology Division, Regional Research Laboratory, Jammu-Tawi 180001, India.
Khan
Inshad Ali
IA
Verma
Vijashoor
V
Kalyan
Narender
N
Qazi
Giulam Nabi
GN
D000995:Antitubercular Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001681:Biological Assay / Q000379:methods; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D050901:Nitrate Reductase / Q000032:analysis* / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D014376:Tuberculosis / Q000188:drug therapy / Q000382:microbiology
D000995:Antitubercular Agents; D009566:Nitrates; D011933:Reagent Kits, Diagnostic; D050901:Nitrate Reductase