title: Biodegradation of azo dyes by genetically engineered azoreductase.
abstract: A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1.1737. The enzyme, with a molecular weight of 18.7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.
authors.affiliation: School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116023, China. wangjing1967@sohu.com
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Yan
authors.forename: Ji-Ti
authors.initials: JT
authors.lastname: Zhou
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Bao
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Yuan
mesh_terms: D001391:Azo Compounds / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000378:metabolism*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005818:Genetic Engineering; D013058:Mass Spectrometry; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D009601:Nitroreductases; D010957:Plasmids
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D017931:DNA Primers; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase