Apoptosis of peripheral blood lymphocytes T in nephrotic children.
The purpose of the study was to assess the intensity of lymphocyte T apoptosis in nephrotic children's peripheral blood before, during and after the treatment with prednisone. The percentages of apoptotic lymphocyte T were determined by flow cytometry using annexin V. The study comprised 26 nephrotic children aged 3-14 years. The controls were 15 healthy children. In acute phase of the nephrotic syndrome before institution of the therapy the mean percentage of apoptotic lymphocytes T was 2.61%. It was significantly higher than that in controls (1.08%). During treatment with prednisone a statistically significant increase in the mean percentage of apoptotic lymphocytes T was observed. After four-week therapy with prednisone and after completion of the treatment the percentages of apoptotic lymphocytes T were 7.96% and 9.51%, respectively. They were significantly higher than those in patients before treatment and in healthy children.
Borzecka
Halina
H
Department of Pediatric Nephrology, Skubiszewski Medical University of Lublin.
Bojarska-Junak
Agnieszka
A
Sikora
Przemysław
P
Majewski
Marek
M
Zajaczkowska
Małgorzata
M
Roliński
Jacek
J
D000208:Acute Disease; D000293:Adolescent; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology; D018655:Lymphocyte Count; D008297:Male; D009404:Nephrotic Syndrome / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*; D011241:Prednisone / Q000627:therapeutic use; D012016:Reference Values; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000473:pathology*
D000893:Anti-Inflammatory Agents; D011241:Prednisone