Calcitonin gene-related peptide receptor expression in healthy and inflamed human pulp tissue.
OBJECTIVE
To use radioreceptor analysis for comparing calcitonin gene-related peptide (CGRP) receptor expression in human pulp tissue samples collected from teeth having a clinical diagnosis of acute irreversible pulpitis, healthy pulps and teeth with induced inflammation.


METHODS
Six pulp samples were obtained from teeth having a clinical diagnosis of acute irreversible pulpitis. Another eight pulp samples were obtained from healthy premolars where extraction was indicated for orthodontic purposes. In four of these premolars, inflammation was induced prior to pulp collection. All the samples were processed and labelled with 125I-CGRP. Binding sites were identified by 125I-CGRP and standard CGRP competition assays.


RESULTS
CGRP receptor expression was found in all human pulp tissue samples. Most receptors were found in the group of pulps from teeth having a clinical diagnosis of acute irreversible pulpitis, followed by the group of pulps having induced inflammation. The least number of receptors was expressed in the group of healthy pulps. The Kruskal-Wallis and Mann-Whitney (post-hoc) tests showed statistically significant differences between the groups (P < 0.05).


CONCLUSIONS
CGRP receptor expression in human pulp tissue is significantly increased during inflammatory phenomena such as acute irreversible pulpitis.
Caviedes-Bucheli
J
J
Graduate Studies Department, School of Dentistry, Pontificia Universidad Javeriana, Bogota, Colombia. javiercaviedes@cable.net.co
Arenas
N
N
Guiza
O
O
Moncada
N A
NA
Moreno
G C
GC
Diaz
E
E
Munoz
H R
HR
D000328:Adult; D001665:Binding Sites; D003782:Dental Pulp / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D020078:Neurogenic Inflammation / Q000378:metabolism; D011671:Pulpitis / Q000378:metabolism*; D011869:Radioligand Assay; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000032:analysis / Q000096:biosynthesis*; D018709:Statistics, Nonparametric
D007457:Iodine Radioisotopes; D018015:Receptors, Calcitonin Gene-Related Peptide