title: Hairy root cultures of Gynostemma pentaphyllum (Thunb.) Makino: a promising approach for the production of gypenosides as an alternative of ginseng saponins.
abstract: Hairy root cultures of Gynostemma pentaphyllum were established by infecting leaf discs with Agrobacterium rhizogenes. The dry biomass of hairy roots grown in MS medium for 49 days was 7.3 g l(-1) with a gypenoside content of 38 mg g(-1) dry wt.
authors.affiliation: Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Chen-Kai
authors.initials: CK
authors.lastname: Chang
authors.forename: Ku-Shang
authors.initials: KS
authors.lastname: Chang
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Lin
authors.forename: Sin-Yie
authors.initials: SY
authors.lastname: Liu
authors.forename: Chien-Yuan
authors.initials: CY
authors.lastname: Chen
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D029774:Gynostemma / Q000235:genetics* / Q000378:metabolism*; D005894:Panax / Q000378:metabolism; D010936:Plant Extracts / Q000096:biosynthesis; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012503:Saponins / Q000096:biosynthesis; D014162:Transfection / Q000379:methods*
chemical_list: D010936:Plant Extracts; D011994:Recombinant Proteins; D012503:Saponins; C474918:gypenoside