Histologic findings after gastrocystoplasty in rabbits.
OBJECTIVE
The aim of this study was to investigate the long-term histologic changes after bladder augmentation with gastric segment in an animal subject.


METHODS
Gastrocystoplasty was performed in 13 young, 3-month-old male rabbits. Open biopsies were taken from the native bladder and the gastric segment preoperatively and at 3, 6, and 12 months postoperatively. Sections were examined with H&E and periodic acid-Schiff (PAS) staining. Indirect immune peroxidase method was additionally applied to detect the carcinoembrionic antigen, the proliferative activity, and the gene for the tumor protein p53 in the epithelium.


RESULTS
On the native bladder, at the 3-month follow-up, polyps, mucosal edema, submucosal fibrosis, and squamous cell metaplasia were detected, which did not change during the follow-up. On the gastric segment, at the 3-month follow-up, parietal cell hyperplasia and inflammatory mucosal overgrowth were detected; at the 6-month follow-up, inflammation or atrophy of the gastric mucosa and colonic-type metaplasia was found. These alterations remained unchanged during later course of follow-up. Neither dysplasia nor malignancy was observed during the 12-month follow-up.


CONCLUSIONS
The present study supports the clinical observations of low cancer risk after gastrocystoplasty and may indicate different effect of gastric secretion on uroepithelium and that of urine on gastric mucosa.
Vajda
Peter
P
Department of Pediatrics, University of Pecs, H-7623 Pecs, Hungary. peter.vajda@aok.pte.hu
Pinter
Andrew B
AB
Magyarlaki
Tamas
T
Vastyan
Attila M
AM
Juhasz
Zsolt
Z
Oberritter
Zsolt
Z
Fathi
Khaled
K
D000818:Animals; D001284:Atrophy; D002471:Cell Transformation, Neoplastic; D005753:Gastric Mucosa / Q000254:growth & development / Q000473:pathology; D006965:Hyperplasia; D007249:Inflammation; D008297:Male; D011183:Postoperative Complications; D011817:Rabbits; D012307:Risk Factors; D013270:Stomach / Q000473:pathology* / Q000637:transplantation*; D016896:Treatment Outcome; D001743:Urinary Bladder / Q000473:pathology* / Q000601:surgery*; D001749:Urinary Bladder Neoplasms / Q000209:etiology; D014549:Urinary Incontinence / Q000601:surgery; D013520:Urologic Surgical Procedures / Q000379:methods*