Mycobacterial lipomannan induces matrix metalloproteinase-9 expression in human macrophagic cells through a Toll-like receptor 1 (TLR1)/TLR2- and CD14-dependent mechanism.
Lipomannans (LM) from various mycobacterial species were found to induce expression and secretion of the matrix metalloproteinase 9 (MMP-9) both in human macrophage-like differentiated THP-1 cells and in primary human macrophages. Inhibition studies using antireceptor-neutralizing antibodies are indicative of a Toll-like receptor 1 (TLR1)/TLR2- and CD14-dependent signaling mechanism. Moreover, LM was shown to down-regulate transcription of the metalloproteinase inhibitor TIMP-1, a major endogenous MMP-9 regulator.
Elass
Elisabeth
E
Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS no. 8576, IFR 118, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France. elisabeth.elass@univ-lille1.fr
Aubry
Laëtitia
L
Masson
Maryse
M
Denys
Agnès
A
Guérardel
Yann
Y
Maes
Emmanuel
E
Legrand
Dominique
D
Mazurier
Joël
J
Kremer
Laurent
L
D000906:Antibodies / Q000494:pharmacology; D002478:Cells, Cultured; D015536:Down-Regulation; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D009161:Mycobacterium / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D000906:Antibodies; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D019715:Tissue Inhibitor of Metalloproteinase-1; C064351:lipomannan; D020780:Matrix Metalloproteinase 9