A proteomic biosensor for enteropathogenic E. coli.
The study of proteins and the molecules with which they interact on an organismwide scale is critical to understanding basic biology, and understanding and improving human health. New platform technologies allowing label-free, quantitative array-based analysis of proteins are particularly desirable. We have developed an analytical technology, reflective interferometry (RI), which provides specific, rapid, and label-free optical detection of biomolecules in complex mixtures. In order to evaluate the suitability of RI for proteomics, we have prepared a series of arrays bearing the extracellular domain of the secreted enteropathogenic Escherichia coli (EPEC) protein Translocated Intimin Receptor (Tir). These arrays are able to selectively detect the extracellular domain of the protein Intimin, Tir's natural binding partner. Furthermore, we demonstrate the use of RI and Tir-functionalized arrays for the selective detection of EPEC directly from culture.
Horner
Scott R
SR
Department of Biochemistry and Biophysics, University of Rochester, NY 14642, USA.
Mace
Charles R
CR
Rothberg
Lewis J
LJ
Miller
Benjamin L
BL
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000032:analysis*; D020869:Gene Expression Profiling / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods; D007422:Intestines / Q000382:microbiology; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D040901:Proteomics / Q000295:instrumentation / Q000379:methods; D011956:Receptors, Cell Surface / Q000032:analysis*; D012031:Refractometry / Q000295:instrumentation / Q000379:methods
D029968:Escherichia coli Proteins; D011956:Receptors, Cell Surface; C109287:Tir protein, E coli