title: Protein crystallography microdiffraction.
abstract: Protein microdiffraction using monochromatic beams is becoming a routine tool at third-generation synchrotron radiation sources. Beam sizes have reached the scale of about 5 microm, with illuminated crystal volumes of approximately 500 microm3, as shown for the case of bovine rhodopsin, which was refined to a resolution of 2.6 A. Progress in X-ray optical systems and instrumentation will enable the method to be extended to smaller beams and smaller crystal volumes.
authors.affiliation: European Synchrotron Radiation Facility, BP 220, F-38043 Grenoble Cedex, France. riekel@esrf.fr
authors.forename: Christian
authors.initials: C
authors.lastname: Riekel
authors.forename: Manfred
authors.initials: M
authors.lastname: Burghammer
authors.forename: Gebhard
authors.initials: G
authors.lastname: Schertler
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D009154:Mutation; D012243:Rhodopsin / Q000737:chemistry* / Q000235:genetics; D017356:Synchrotrons / Q000295:instrumentation; D014961:X-Ray Diffraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D000682:Amyloid; D012243:Rhodopsin