Modified western blot technique in fast detection of heme oxygenase (HO-1/HO-2) in various tissues and organs of experimental animals.
The present study was aimed at determining all indispensable conditions required to detect heme oxygenase (HO) with western blot technique. Our modified immunoblotting method allowed the detection of HO after 2 hours of electro-transferring onto nitrocellulose membranes that evidently shortened the time of research study without any loss of sensitivity and specificity to detect HO in various tissues and organs of experimental animals. HO was detected in the brain, heart, kidney, liver, lung, spleen, testis, and thymus of the examined mouse and rat, in a quantity for providing evidence that this modified immunoblotting technique is sensitive enough to elicit the existence of this enzyme in various animals' tissues and organs. In conclusion, our modified western blot method permits the fast detection of HO that may be useful in further more advanced quantitative studies.
Andres
Marta M
MM
Isotope Laboratory, Institute of Agricultural Medicine, Lublin.
Luszczki
Jarogniew J
JJ
D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D006419:Heme Oxygenase (Decyclizing) / Q000032:analysis*; D051547:Heme Oxygenase-1; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D051381:Rats; D017208:Rats, Wistar
D008565:Membrane Proteins; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; C105245:heme oxygenase-2