IL-2 production by dendritic cells is not critical for the activation of cognate and innate effectors in draining lymph nodes.
Dendritic cells (DC) are unique antigen-presenting cells capable of triggering NK cell effector functions and priming naive T cells in vivo. Microbial stimulation induces early IL-2 production by mouse DC. Previous reports demonstrated that IL-2 is enriched at the site of DC/T cell interaction and promotes allogeneic T cell proliferation. However, the direct role of DC-derived IL-2 in the differentiation of cytotoxic T lymphocytes and in NK cell triggering in vivo has not been investigated. Lipopolysaccharide (LPS) stimulation of mouse bone marrow-derived DC results in early IL-2 production unless IL-4 is introduced in DC cultures. Here we show that IL-2 produced by LPS-activated DC is dispensable for cognate T cell responses since IL-2 loss of function DC elicit OVA-specific Tc1 effector and memory lymphocytes in draining lymph nodes in a setting where ex vivo cultured DC do not transfer antigens to host DC. Moreover, adoptively transferred IL-2 loss of function DC maintain their capacity to trigger NK cell proliferation/recruitment in lymph nodes. Therefore, immediate inducible IL-2 production by DC following microbial infection might play a regulatory role at ports of entry rather than in secondary lymphoid organs.
Schartz
Noël E C
NE
Unité d'Immunologie, ERM0208 INSERM, Institut Gustave Roussy, Villejuif, France. noel.schartz@noos.fr
Chaput
Nathalie
N
Taieb
Julien
J
Bonnaventure
Pierre
P
Trébeden-Nègre
Hélène
H
Terme
Magali
M
Ménard
Cédric
C
Lebbé
Céleste
C
Schimpl
Anneliese
A
Ardouin
Patrice
P
Zitvogel
Laurence
L
D019264:Adoptive Transfer; D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D007376:Interleukin-2 / Q000172:deficiency / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D007376:Interleukin-2; D008070:Lipopolysaccharides