title: Molecular regulation of histone H3 trimethylation by COMPASS and the regulation of gene expression.
abstract: The Set1-containing complex COMPASS, which is the yeast homolog of the human MLL complex, is required for mono-, di-, and trimethylation of lysine 4 of histone H3. We have performed a comparative global proteomic screen to better define the role of COMPASS in histone trimethylation. We report that both Cps60 and Cps40 components of COMPASS are required for proper histone H3 trimethylation, but not for proper regulation of telomere-associated gene silencing. Purified COMPASS lacking Cps60 can mono- and dimethylate but is not capable of trimethylating H3(K4). Chromatin immunoprecipitation (ChIP) studies indicate that the loss subunits of COMPASS required for histone trimethylation do not affect the localization of Set1 to chromatin for the genes tested. Collectively, our results suggest a molecular requirement for several components of COMPASS for proper histone H3 trimethylation and regulation of telomere-associated gene expression, indicating multiple roles for different forms of histone methylation by COMPASS.
authors.affiliation: Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St. Louis, Missouri 63104, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Schneider
authors.forename: Adam
authors.initials: A
authors.lastname: Wood
authors.forename: Jung-Shin
authors.initials: JS
authors.lastname: Lee
authors.forename: Rebecca
authors.initials: R
authors.lastname: Schuster
authors.forename: Jeff
authors.initials: J
authors.lastname: Dueker
authors.forename: Courtney
authors.initials: C
authors.lastname: Maguire
authors.forename: Selene K
authors.initials: SK
authors.lastname: Swanson
authors.forename: Laurence
authors.initials: L
authors.lastname: Florens
authors.forename: Michael P
authors.initials: MP
authors.lastname: Washburn
authors.forename: Ali
authors.initials: A
authors.lastname: Shilatifard
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D011495:Histone-Lysine N-Methyltransferase; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D046912:Multiprotein Complexes; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D016615:Telomere; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; D046912:Multiprotein Complexes; D021122:Protein Subunits; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D011495:Histone-Lysine N-Methyltransferase; C411250:SET1 protein, S cerevisiae