Requirements for heterologous production of a complex metalloenzyme: the membrane-bound [NiFe] hydrogenase.
By taking advantage of the tightly clustered genes for the membrane-bound [NiFe] hydrogenase of Ralstonia eutropha H16, broad-host-range recombinant plasmids were constructed carrying the entire membrane-bound hydrogenase (MBH) operon encompassing 21 genes. We demonstrate that the complex MBH biosynthetic apparatus is actively produced in hydrogenase-free hosts yielding fully assembled and functional MBH protein.
Lenz
Oliver
O
Institut für Biologie, Humboldt-Universität zu Berlin, Chausseestrasse 117, D-10115 Berlin, Germany.
Gleiche
Andrea
A
Strack
Angelika
A
Friedrich
Bärbel
B
D020118:Cupriavidus necator / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D006864:Hydrogenase / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
D011994:Recombinant Proteins; D006864:Hydrogenase