Fluorometric and fluorescent image analysis methods for determination of lipid hydroperoxides in oil models with 3-perylene diphenylphosphine (3-PeDPP).
Fluorometric and fluorescent image analysis methods with 3-perylene diphenylphosphine (3-PeDPP) were developed to measure lipid hydroperoxides in refined tuna oil. The fluorescence intensity of 3-perylene diphenylphosphineoxide (3-PeDPPO, the corresponding peroxidation product of 3-PeDPP) has been used for assessing lipid hydroperoxides in refined tuna oil models, and the results are compared to those of a classical method using ferric-xylenol orange complex. There were good correlations between the lipid hydroperoxide contents determined by the 3-PeDPPO fluorescence and the xylenol orange methods (R(2) = 0.984). Moreover, the novel probe 3-PeDPP and the fluorescent image analysis enabled us to evaluate two-dimensionally the lipid hydroperoxide contents in tuna oil droplets. These results suggest that the fluorometric and fluorescent image analyses using 3-PeDPP would be suitable for batch and two-dimensional determinations of lipid hydroperoxides in oil and oil-containing materials.
Chotimarkorn
Chatchawan
C
Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato, Japan.
Ohshima
Toshiaki
T
Ushio
Hideki
H
D000818:Animals; D005395:Fish Oils / Q000737:chemistry*; D005456:Fluorescent Dyes; D008054:Lipid Peroxides / Q000032:analysis*; D008856:Microscopy, Fluorescence; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D010569:Perylene / Q000031:analogs & derivatives*; D010720:Phosphines; D013050:Spectrometry, Fluorescence; D024505:Tocopherols / Q000032:analysis; D014413:Tuna
C504951:3-perylene diphenylphosphine; D005395:Fish Oils; D005456:Fluorescent Dyes; D008054:Lipid Peroxides; D010720:Phosphines; D010569:Perylene; D024505:Tocopherols; D010100:Oxygen