title: Method of cell transplantation promoting the organization of intraarterial thrombus.
abstract: BACKGROUND
  Endovascular aortic repairs have been developed as less invasive treatments for aortic aneurysms. Some aneurismal cavities, however, remain without organization, causing a re-expansion of the aneurysms. We studied cell transplantation into the aneurismal sac to promote the organization of thrombus for the complete healing of aneurysms.
  
  
  RESULTS
  Skin fibroblasts and skeletal myoblasts were isolated from rats for cell transplantation. An intraarterial thrombus model was made by ligation of the carotid artery. Culture medium (medium group, n=11), collagen gel (gel group, n=11), fibroblasts with collagen gel (F group, n=15), myoblasts with collagen gel (M group, n=12), or mixture of fibroblasts and myoblasts with collagen gel (F+M group, n=14) were injected into the thrombus. After 28 days, histologically, the arterial lumens of the F and M groups were partly filled with fibrous tissues, whereas in the F+M group organization was almost completed and luminal sizes diminished. Immunohistochemical staining demonstrated that alpha-smooth muscle actin-positive cells were more abundantly contained in the organized area of the F+M group than in the other groups. We also analyzed cellular function in vitro with immunofluorescence; coculture of fibroblasts and myoblasts showed that the fraction of alpha-smooth muscle actin-positive fibroblasts increased. This phenomenon accounts for the rapid organization of thrombus in the F+M group in vivo.
  
  
  CONCLUSIONS
  Cell transplantation accelerated thrombus organization. Especially, myoblasts enhanced differentiation of fibroblasts into myofibroblasts, contributing to rapid thrombus organization. Cell transplantation into unorganized spaces seems applicable to endovascular treatment of aneurysms.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Mie University School of Medicine, Tsu, Mie 514-8507, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Hirano
authors.forename: Takatsugu
authors.initials: T
authors.lastname: Shimono
authors.forename: Kyoko
authors.initials: K
authors.lastname: Imanaka-Yoshida
authors.forename: Keiichi
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Kazuya
authors.initials: K
authors.lastname: Fujinaga
authors.forename: Masaki
authors.initials: M
authors.lastname: Kajimoto
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Miyake
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Nishikawa
authors.forename: Toshimichi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Atsumasa
authors.initials: A
authors.lastname: Uchida
authors.forename: Hideto
authors.initials: H
authors.lastname: Shimpo
authors.forename: Isao
authors.initials: I
authors.lastname: Yada
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hirata
mesh_terms: D000783:Aneurysm / Q000473:pathology; D000818:Animals; D002340:Carotid Artery Diseases / Q000473:pathology; D002341:Carotid Artery Thrombosis / Q000473:pathology / Q000601:surgery*; D002454:Cell Differentiation; D002478:Cells, Cultured / Q000637:transplantation; D018920:Coculture Techniques; D003094:Collagen / Q000008:administration & dosage; D005347:Fibroblasts / Q000637:transplantation*; D005782:Gels; D007269:Injections, Intra-Arterial; D032342:Muscle Cells / Q000637:transplantation*; D018482:Muscle, Skeletal / Q000166:cytology; D032446:Myoblasts / Q000637:transplantation*; D051381:Rats; D011917:Rats, Inbred Lew; D012867:Skin / Q000166:cytology
chemical_list: D005782:Gels; D003094:Collagen