Pathogenesis and phylogenetic analyses of canine distemper virus strain 007Lm, a new isolate in dogs.
The pathogenesis of a new isolate of canine distemper virus (CDV), strain 007Lm, was investigated from lymph node tissue by using Vero cells that express canine signalling lymphocyte activation molecules with a tag (Vero-DST) in dogs. Two CDV sero-negative Beagle dogs were inoculated intranasally and intraconjunctively with a virus suspension. Both infected dogs showed clinical signs of severe bloody diarrhea, conjunctivitis, ocular discharge, nasal discharge and coughing, lymphopenia, fever and weight loss. Titers of CDV-IgM and CDV-IgG in the blood were measured. CDV was detected by using reverse transcriptase-PCR and was recovered in swabs from one dog from 9 days and from the other dogs from 10 days after inoculation. Molecular and phylogenetic analyses of H and P genes showed that nucleotide and amino acid sequences of these genes of strain 007Lm after isolation in Vero-DST cells are identical to those of the original virus from fresh tissue and that strain 007Lm joins to the Asia 2 group cluster of CDV strains that is distinct from other clusters. These results indicate that (1) CDV strain 007Lm isolated in Vero-DST cells is virulent, (2) nucleotide and amino acid sequences of H and P genes of strain 007Lm do not change after isolation in Vero-DST cells compared with the original virus from fresh tissue and (3) strain 007Lm isolated from a vaccinated dog belongs to a cluster far from the vaccine strains in the phylogenetic trees of H and P genes.
Lan
N T
NT
Department of Veterinary Pathology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
Yamaguchi
R
R
Furuya
Y
Y
Inomata
A
A
Ngamkala
S
S
Naganobu
K
K
Kai
K
K
Mochizuki
M
M
Kobayashi
Y
Y
Uchida
K
K
Tateyama
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002522:Chlorocebus aethiops; D004216:Distemper / Q000821:virology*; D004217:Distemper Virus, Canine / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D004285:Dogs; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008198:Lymph Nodes / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012699:Serology; D013997:Time Factors; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014764:Viral Proteins