title: Nocardia jiangxiensis sp. nov. and Nocardia miyunensis sp. nov., isolated from acidic soils.
abstract: The taxonomic positions of two soil actinomycetes, strains 117(T) and 43401(T), provisionally assigned to the genus Nocardia were determined in a polyphasic study. The organisms were found to have phenotypic properties typical of members of the genus Nocardia, and formed a distinct branch in the Nocardia 16S rRNA gene tree. It was evident from the phylogenetic data that the isolates were most closely, albeit loosely, associated with the type strains of Nocardia nova and Nocardia pseudobrasiliensis. However, all four of these organisms were readily distinguishable from one another using DNA-DNA relatedness and phenotypic data. It is evident from the genotypic and phenotypic data that the two isolates should be recognized as novel species of the genus Nocardia. It is proposed, therefore, that strains 117(T) (=CGMCC 4.1904(T)=JCM 12860(T)) and 43401(T) (=CGMCC 4.1905(T)=JCM 12861(T)) be classified in the genus Nocardia as the type strains of Nocardia miyunensis sp. nov. and Nocardia jiangxiensis sp. nov., respectively.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009615:Nocardia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D028223:Pinus; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014197:Trees
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S