The study of 3-dimensional structures of IgG with atomic force microscopy.
OBJECTIVE
To detect 3-dimensional images of anti-N-methyl-D-aspartate receptor Nr1 (NMDAr1) polycolonal IgG affixed on mica in physiological environment.


METHODS
The images and data were obtained from a contact mode and commercial Si3N4 probed tip by using atomic force microscope (AFM).


RESULTS
The anti-NMDAr1 polycolonal IgG has a characteristic structure described as an ellipse spherical shape of 136.4 A x 62.8 A x 26.1 A. On the section of the ellipse edge there were two peaks about 13 nm in width.


CONCLUSIONS
Using AFM to investigate biomacromolecule can make us deeply understand the structure of IgG, which will instruct us to detect the membrane receptor protein as a labelling agent.
Yu
Yi-gang
YG
Department of Neurosurgery, Zhujiang Hospital, South Medical University, Guangzhou 510282, China. yu-yg@tom.com
Xu
Ru-xiang
RX
Jiang
Xiao-dan
XD
Ke
Yi-quan
YQ
D000327:Adsorption; D000538:Aluminum Silicates; D017979:Gold Colloid; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D016194:Receptors, N-Methyl-D-Aspartate / Q000737:chemistry*
D000538:Aluminum Silicates; D017979:Gold Colloid; D007074:Immunoglobulin G; D016194:Receptors, N-Methyl-D-Aspartate; C011934:mica