Hydroxyl radical formation in skeletal muscle of rats with glucocorticoid-induced myopathy.
Steroid myopathy is a well-known adverse effect of glucocorticoids that causes muscle weakness and atrophy; however, its pathogenic mechanism is still unclear. Recently, oxidative stress was reported to contribute to steroid myopathy, but there is no report that actually attempts to measure hydroxyl radical. I developed an animal model of steroid myopathy in rat with dexamethasone (9-Fluoro-11beta,17, 21-trihydroxy-16alpha-methylpregna-1,4-diene-3,20-dione), and measured hydroxyl radical using the salicylate trapping method. There was significant dose-dependent relation between both 2,5- and 2,3-dihydroxybenzoic acids and dexamethasone in the treated group, compared to the control group. These results suggest that hydroxyl radical plays a role in the pathogenesis of steroid myopathy.
Konno
Shingo
S
Department of Neurology, Toho University School of Medicine, Oohashi Hospital, 2-17-6 Oohashi, 153-8515, Meguro-ku, Tokyo, Japan. d500372@oha.toho-u.ac.jp
D000818:Animals; D001835:Body Weight; D003402:Creatine Kinase / Q000097:blood; D003907:Dexamethasone / Q000633:toxicity*; D005247:Feeding Behavior; D017665:Hydroxyl Radical / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009135:Muscular Diseases / Q000139:chemically induced*; D051381:Rats; D017208:Rats, Wistar
D017665:Hydroxyl Radical; D003907:Dexamethasone; D003402:Creatine Kinase