Chronic intracerebroventricular infusion of lipopolysaccharide: effects of ibuprofen treatment and behavioural and histopathological correlates.
Twenty male Wistar rats were trained under an alternating-lever cyclic-ratio (ALCR) schedule of food reinforcement. When responding showed no trends, each subject was subcutaneously implanted with an Alzet osmotic mini-pump, connected to a chronic indwelling cannula extending into the lateral ventricle of the brain. The mini-pumps were primed to infuse 0.25 microl lipopolysaccharide (LPS) (1.0 microg/0.25 ml) or 0.25 microl artificial cerebrospinal fluid (aCSF) per hour and were implanted for 28 days. LPS infusion produced behavioural deficits which chronic ibuprofen treatment (40 mg/kg every 12 h) alleviated. Infusion of LPS induced R 1282-positive amyloid deposits, and activation of microglia and astrocytes. Ibuprofen treatment reduced the numbers of activated microglia, and withdrawal of ibuprofen resulted in an increase in activated microglia; however, ibuprofen treatment had no effect on numbers of activated astrocytes in the LPS-infused subjects.
Richardson
R L
RL
School of Psychology, University of Ulster, Shore Road, Newtownabbey, Co. Antrim, N. Ireland, UK.
Kim
E-M
EM
Gardiner
T
T
O'Hare
E
E
D016229:Amyloid beta-Peptides / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000941:Antigens / Q000378:metabolism; D001253:Astrocytes / Q000473:pathology; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000473:pathology*; D005502:Food; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007052:Ibuprofen / Q000494:pharmacology*; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D008568:Memory / Q000187:drug effects; D017628:Microglia / Q000473:pathology; D016874:Neurofibrillary Tangles / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012055:Reinforcement Schedule; D012054:Reinforcement, Psychology
C056167:Alzheimer's disease antigen; D016229:Amyloid beta-Peptides; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000941:Antigens; D005904:Glial Fibrillary Acidic Protein; D008070:Lipopolysaccharides; D007052:Ibuprofen