title: Streptomyces bangladeshensis sp. nov., isolated from soil, which produces bis-(2-ethylhexyl)phthalate.
abstract: The taxonomic position of an actinomycete strain isolated from soil from Natore, Bangladesh, was examined by using a polyphasic approach. The strain, designated AAB-4(T), was assigned to the genus Streptomyces on the basis of chemical and morphological criteria. It formed Rectiflexibiles aerial hyphae that carried long chains of rounded spores. The 16S rRNA gene of strain AAB-4(T) was sequenced directly and then compared with those of previously studied streptomycetes following the generation of two phylogenetic trees by using maximum-likelihood and neighbour-joining algorithms. This confirmed the assignment of the novel strain to the genus Streptomyces. This strain showed a high level of 16S rRNA gene sequence similarity to Streptomyces thermoviolaceus, Streptomyces thermodiastaticus and Streptomyces longisporus, among others, but could be distinguished from them by phenotypic and physiological traits. This micro-organism produces bis-(2-ethylhexyl)phthalate, an antibacterial and antifungal agent. It is proposed that strain AAB-4(T) be classified as a novel species within the genus Streptomyces, as Streptomyces bangladeshensis sp. nov. (type strain, AAB-4(T)=LMG 22738(T)=NRRL B-24326(T)).
authors.affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
authors.forename: M Abdul Alim
authors.initials: MAA
authors.lastname: Al-Bari
authors.affiliation: Laboratory of Molecular Biotechnology, Department of Biotechnology, The University of Tokyo, Yayoi 113-8657, Japan.|Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
authors.forename: M Shah Alam
authors.initials: MSA
authors.lastname: Bhuiyan
authors.affiliation: Department of Molecular Biology and Biotechnology, Institute for Biomedical Research, UNAM, A.P. 70228, Mexico, D.F., 04510, Mexico.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Flores
authors.affiliation: Department of Molecular Biology and Biotechnology, Institute for Biomedical Research, UNAM, A.P. 70228, Mexico, D.F., 04510, Mexico.
authors.forename: Pavel
authors.initials: P
authors.lastname: Petrosyan
authors.affiliation: Department of Zoology, Institute of Biology, UNAM, México, D.F., 04510, Mexico.
authors.forename: Martín
authors.initials: M
authors.lastname: García-Varela
authors.affiliation: Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.
authors.forename: M Anwar Ul
authors.initials: MAU
authors.lastname: Islam
mesh_terms: D001459:Bangladesh; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D010795:Phthalic Acids / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000502:physiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010795:Phthalic Acids; D012336:RNA, Ribosomal, 16S