title: Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis.
abstract: An Escherichia coli-Laribacter hongkongensis shuttle vector (pPW380) was constructed by ligating the 4701-bp EcoRI digested fragment of pHLHK8 to EcoRI digested pBK-CMV. An E. coli-L. hongkongensis inducible expression shuttle vector was further constructed by ligating a 2105-bp fragment that contains the tetracycline repressor and tetracycline-inducible promoter region of pALC2084 to the 8897-bp fragment of pPW380, deletion of the green fluorescent protein gene, and insertion of a multiple cloning site. This inducible expression system was able to express two commonly used reporter genes, the green fluorescent protein gene and the glutathione S-transferase gene, efficiently in E. coli and L. hongkongensis.
authors.affiliation: Department of Microbiology, Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital Compound, Pokfulam Road, Hong Kong.
authors.forename: Patrick C Y
authors.initials: PC
authors.lastname: Woo
authors.forename: Shirley S L
authors.initials: SS
authors.lastname: Ma
authors.forename: Jade L L
authors.initials: JL
authors.lastname: Teng
authors.forename: Maria W S
authors.initials: MW
authors.lastname: Li
authors.forename: Richard Y T
authors.initials: RY
authors.lastname: Kao
authors.forename: Susanna K P
authors.initials: SK
authors.lastname: Lau
authors.forename: Kwok-Yung
authors.initials: KY
authors.lastname: Yuen
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005759:Gastroenteritis / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016870:Neisseriaceae Infections / Q000382:microbiology; D010957:Plasmids; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
chemical_list: D049452:Green Fluorescent Proteins; D005982:Glutathione Transferase