Zingerone as an antioxidant against peroxynitrite.
Peroxynitrite (ONOO(-)), formed from the reaction of superoxide ((*)O(2)(-)) and nitric oxide ((*)NO), induces cellular and tissue injury, resulting in several human diseases such as stroke, Alzheimer's disease, and atherosclerosis. Due to the lack of endogenous enzymes responsible for ONOO(-) scavenging activity, finding a specific ONOO(-) scavenger is of considerable importance. In this study we examined the scavenging effects of zingerone from ginger against ONOO(-), intracellular RS (reactive species), and ONOO(-). The data show that zingerone can efficiently scavenge native ONOO(-) as well as ONOO(-) derived from the peroxynitrite donor 3-morpholinosydnonimine hydrochloride (SIN-1). Zingerone inhibited the formation of ONOO(-)-mediated tyrosine nitration through electron donation, nitration of bovine serum albumin (BSA) by ONOO(-), and intracellular RS and ONOO(-). The present study suggests that zingerone has an efficient ONOO(-) scavenging ability, which may be a potent ONOO(-) scavenger for the protection of the cellular defense activity against ONOO(-)- involved diseases.
Shin
Sang-Guk
SG
Department of Internal Medicine, College of Oriental Medicine, Dong-Guk University, Pusan National University, Busan, Korea.
Kim
Ji Young
JY
Chung
Hae Young
HY
Jeong
Ji-Cheon
JC
D000975:Antioxidants / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000494:pharmacology; D030421:Peroxynitrous Acid / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry
D000975:Antioxidants; D016166:Free Radical Scavengers; D030421:Peroxynitrous Acid; D012710:Serum Albumin, Bovine; C002744:3-nitrotyrosine; D014443:Tyrosine; C013738:zingerone; D006139:Guaiacol