Nucleic acid amplification-based techniques for pathogen detection and identification.
Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and amplification techniques for pathogen detection and identification, including the polymerase chain reaction, nucleic acid sequence-based amplification, strand displacement amplification and the ligase chain reaction. The emergence of newer technologies, such as lab-on-a-chip devices and photo-cleavable linkers, is also discussed.
Monis
Paul T
PT
Australian Water Quality Centre, South Australian Water Corporation, Microbiology Unit, Salisbury, SA 5108, Australia. paul.monis@sawater.com.au
Giglio
Steven
S
D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis* / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation; D021141:Nucleic Acid Amplification Techniques; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D037521:Virulence Factors / Q000235:genetics
D004269:DNA, Bacterial; D004279:DNA, Viral; D037521:Virulence Factors