Detection of nitrous oxide in the neuronal nitric oxide synthase reaction by gas chromatography-mass spectrometry.
Using headspace gas chromatography-mass spectrometry, we detected significant amounts of nitrous oxide in the reaction products of the monooxygenase reaction catalyzed by neuronal nitric oxide synthase. Nitrous oxide is a dimerization product of nitroxyl anion; its presence in the reaction products indicates that the nitroxyl anion is a product of the neuronal nitric oxide synthase-catalyzed reaction.
Ishimura
Yuzuru
Y
Department of Biochemistry, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA.
Gao
Ying Tong
YT
Panda
Satya P
SP
Roman
Linda J
LJ
Masters
Bettie Sue S
BS
Weintraub
Susan T
ST
D000818:Animals; D004926:Escherichia coli; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D009609:Nitrous Oxide / Q000737:chemistry* / Q000378:metabolism*
D052248:Nitric Oxide Synthase Type I; D009609:Nitrous Oxide