Human primordial germ cells and embryonic germ cells, and their use in cell therapy.
Human embryonic germ (hEG) cells derive from the transformation of primordial germ cells (PGCs) under appropriate culture conditions with embryonic fibroblast feeder cells. Although the pluripotent and proliferative capacity of hEG cells is thought to be equivalent to that of human embryonic stem (hES) cells, the difficulties of isolating and maintaining hEG cell lines in vitro have restricted their availability for experimental use. Despite this, some of the factors involved in PGC development, their transformation into embryonic germ cells and the differentiation of embryonic germ cells to specific cell phenotypes have been explored. The potential use of hEG cells in cell therapy applications will, however, depend on a more thorough understanding of how to derive and maintain these cells in vitro.
Aflatoonian
Behrouz
B
Centre for Stem Cell Biology, University of Sheffield, Sheffield, S10 2UH, UK.
Moore
Harry
H
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D017690:Cell Transplantation / Q000379:methods*; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology; D005854:Germ Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology; D044968:Regenerative Medicine / Q000379:methods; D013234:Stem Cells / Q000166:cytology