2-(2-Selenocyanic acid ethyl ester)-1H-benz[de] isoquinoline-1,3-(2H)-dione, synthesis photophysics and interaction with bovine serum albumin: a spectroscopic approach.
The compound 2-(2-selenocyanic acid ethyl ester)-1H-benz[de] isoquinoline-1,3-(2H)-dione (SEBID), a ubiquitous, bioactive naphthalimide derivative is expected to possess an anticancer, anti-tumor and other important therapeutic activities of significant potency with low systematic toxicity. In this paper, the synthesis of the compound, photophysics of the newly prepared naphthalimide derivative and its interaction with model transport protein Bovine serum albumin (BSA) have been reported using the absorption and steady state fluorescence spectroscopic techniques exploiting the intrinsic fluorescence emission properties of BSA as a probe. Interaction of this organoselenium compound in different dioxane-water mixtures with increase in the polarity of the medium has been studied spectroscopically. Interaction of SEBID with BSA leads to a dramatic decrease in the fluorescence intensity of BSA, which suggests the binding of SEBID with the tryptophan residue of BSA. Furthermore, different thermodynamic parameters for SEBID-BSA interaction have been calculated. Rationalization of the data has been attempted, particularly in relation to prospective applications in the biomedical research.
Ghosh
Sujit Kumar
SK
Department of Chemistry, Jadavpur University, Raja S.C. Mallick Road, Calcutta 700032, India. sujitju@yahoo.co.in
Hossain
Sk Ugir
SU
Bhattacharya
Sudin
S
Bhattacharya
Subhash Chandra
SC
D007546:Isoquinolines; D016566:Organoselenium Compounds; D010777:Photochemistry; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*; D013816:Thermodynamics
C507059:2-(2-selenocyanic acid ethyl ester)-1H-benz(de) isoquinoline-1,3-(2H)-dione; D007546:Isoquinolines; D016566:Organoselenium Compounds; D012710:Serum Albumin, Bovine