Different profile of cytokine synthesis according to the severity of acute pancreatitis.
OBJECTIVE
To evaluate the cellular synthetic ability of cytokines involved in pro- and anti-inflammatory reactions in patients with AP.


METHODS
Sixty-seven patients with AP (16 severe, 51 mild) and 10 controls were included in the study. Cultures of whole blood were performed in samples obtained within the first 72 h from the onset of pain. Serum levels of interleukins (IL) 6, 8, 10, and TNF-alpha were measured at baseline and in the supernatant of cultures with (functional reserve) or without stimulation with phytohemaglutinin.


RESULTS
Basal levels of cytokines were significantly higher in patients with severe AP. A significant increase of all pro-inflammatory cytokines vs basal levels was observed in the supernatant after 24 h of whole blood cultures in patients, but not in controls. In contrast, IL-10 increased significantly in the supernatant of cultures only in patients with mild AP. Cells showed a statistically significant functional reserve for all IL in patients with mild, but only for pro-inflammatory cytokines in patients with severe AP.


CONCLUSIONS
A marked activation of immune system may be observed in patients with AP, being balanced between pro- and anti-inflammatory cytokines in patients with mild but not severe AP. A reduced functional reserve for the synthesis of IL-10 may be observed in patients with severe AP, which might lead to a worst prognosis.
Laveda
Raquel
R
Department of Gastroenterology, Hospital General Universitario de Alicante, Pintor Baeza s/n, Alicante 03010, Spain.
Martinez
Juan
J
Munoz
Carlos
C
Penalva
Juan-Carlos
JC
Saez
Jesus
J
Belda
German
G
Navarro
Salvador
S
Feu
Faust
F
Mas
Anton
A
Palazon
Jose-Ma
JM
Sanchez-Paya
Jose
J
Such
Jose
J
Perez-Mateo
Miguel
M
D000208:Acute Disease; D000328:Adult; D015415:Biomarkers; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism*; D005260:Female; D006801:Humans; D007107:Immune System / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010195:Pancreatitis / Q000276:immunology* / Q000378:metabolism; D012720:Severity of Illness Index
D015415:Biomarkers; D016207:Cytokines