title: Transmembrane adaptor molecules: a new category of lymphoid-cell markers.
abstract: Transmembrane adaptor proteins (of which 7 have been identified so far) are involved in receptor signaling in immune cells. They have only a short extracellular region, with most of the molecule comprising a substantial intracytoplasmic region carrying multiple tyrosine residues that can be phosphorylated by Src- or Syk-family kinases. In this paper, we report an immunohistologic study of 6 of these molecules in normal and neoplastic human tissue sections and show that they are restricted to subpopulations of lymphoid cells, being present in either T cells (LAT, LIME, and TRIM), B cells (NTAL), or subsets of both cell types (PAG and SIT). Their expression in neoplastic lymphoid cells broadly reflects that of normal lymphoid tissue, including the positivity of plasma cells and myeloma/plasmacytoma for LIME, NTAL, PAG, and SIT. However, this study also revealed some reactions that may be of diagnostic/prognostic value. For example, lymphocytic lymphoma and mantle-cell lymphoma showed similar profiles but differed clearly from follicle-center lymphoma, whereas PAG tended to be selectively expressed in germinal center-derived subsets of diffuse large B-cell lymphoma. These molecules represent a potentially important addition to the panel of immunophenotypic markers detectable in routine biopsies that can be used in hematopathologic studies.
authors.affiliation: Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, OX3 9DU, United Kingdom.
authors.forename: Sara
authors.initials: S
authors.lastname: Tedoldi
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Paterson
authors.forename: Martin-Leo
authors.initials: ML
authors.lastname: Hansmann
authors.forename: Yasodha
authors.initials: Y
authors.lastname: Natkunam
authors.forename: Thomas
authors.initials: T
authors.lastname: Rüdiger
authors.forename: Pavla
authors.initials: P
authors.lastname: Angelisova
authors.forename: Ming Q
authors.initials: MQ
authors.lastname: Du
authors.forename: Helen
authors.initials: H
authors.lastname: Roberton
authors.forename: Giovanna
authors.initials: G
authors.lastname: Roncador
authors.forename: Lydia
authors.initials: L
authors.lastname: Sanchez
authors.forename: Michela
authors.initials: M
authors.lastname: Pozzobon
authors.forename: Noraidah
authors.initials: N
authors.lastname: Masir
authors.forename: Richard
authors.initials: R
authors.lastname: Barry
authors.forename: Stefano
authors.initials: S
authors.lastname: Pileri
authors.forename: David Y
authors.initials: DY
authors.lastname: Mason
authors.forename: Teresa
authors.initials: T
authors.lastname: Marafioti
authors.forename: Václav
authors.initials: V
authors.lastname: Horejsí
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis*; D001402:B-Lymphocytes / Q000737:chemistry / Q000473:pathology; D015415:Biomarkers / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008214:Lymphocytes / Q000737:chemistry* / Q000473:pathology; D008223:Lymphoma / Q000737:chemistry* / Q000145:classification* / Q000175:diagnosis; D008565:Membrane Proteins / Q000032:analysis*; D011379:Prognosis; D013601:T-Lymphocytes / Q000737:chemistry / Q000473:pathology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; D008565:Membrane Proteins