T cell epitope-containing peptides of the major dog allergen Can f 1 as candidates for allergen immunotherapy.
One prerequisite for developing peptide-based allergen immunotherapy is knowing the T cell epitopes of an allergen. In this study, human T cell reactivity against the major dog allergen Can f 1 was investigated to determine peptides suitable for immunotherapy. Seven T cell epitope regions (A-G) were found in Can f 1 with specific T cell lines and clones. The localization of the epitope regions shows similarities with those of the epitopes found in Bos d 2 and Rat n 1. On average, individuals recognized three epitopes in Can f 1. Our results suggest that seven 16-mer peptides (p15-30, p33-48, p49-64, p73-88, p107-122, p123-138, and p141-156), each from one of the epitope regions, show widespread T cell reactivity in the population studied, and they bind efficiently to seven HLA-DRB1 molecules (DRB1*0101, DRB1*0301, DRB1*0401, DRB1*0701, DRB1*1101, DRB1*1301, and DRB1*1501) predominant in Caucasian populations. Therefore, these peptides are potential candidates for immunotherapy of dog allergy.
Immonen
Anu
A
Department of Clinical Microbiology, University of Kuopio, Kuopio, Finland. Anu.Immonen@uku.fi
Farci
Sandrine
S
Taivainen
Antti
A
Partanen
Jukka
J
Pouvelle-Moratille
Sandra
S
Närvänen
Ale
A
Kinnunen
Tuure
T
Saarelainen
Soili
S
Rytkönen-Nissinen
Marja
M
Maillere
Bernard
B
Virtanen
Tuomas
T
D000485:Allergens / Q000276:immunology* / Q000627:therapeutic use; D000818:Animals; D052179:Antigens, Plant; D002478:Cells, Cultured; D004285:Dogs; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology; D059811:HLA-DRB1 Chains; D006801:Humans; D006967:Hypersensitivity / Q000628:therapy; D007167:Immunotherapy / Q000379:methods*; D010446:Peptide Fragments / Q000276:immunology* / Q000627:therapeutic use; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D000485:Allergens; D052179:Antigens, Plant; D018984:Epitopes, T-Lymphocyte; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C553502:HLA-DRB1*03:01 antigen; C553505:HLA-DRB1*04:01 antigen; D010446:Peptide Fragments; C091462:allergen Can f I