title: Microsatellite mapping of a Triticum urartu Tum. derived powdery mildew resistance gene transferred to common wheat (Triticum aestivum L.).
abstract: A powdery mildew resistance gene from Triticum urartu Tum. accession UR206 was successfully transferred into hexaploid wheat (Triticum aestivum L.) through crossing and backcrossing. The F1 plants, which had 28 chromosomes and an average of 5.32 bivalents and 17.36 univalents in meiotic pollen mother cells (PMC), were obtained through embryos rescued owing to shriveling of endosperm in hybrid seed of cross Chinese Spring (CS) x UR206. Hybrid seeds were produced through backcrossing F1 with common wheat parents. The derivative lines had normal chromosome numbers and powdery mildew resistance similar to the donor UR206, indicating that the powdery mildew resistance gene originating from T. urartu accession UR206 was successfully transferred and expressed in a hexaploid wheat background. Genetic analysis indicated that a single dominant gene controlled the powdery mildew resistance at the seedling stage. To map and tag the powdery mildew resistance gene, 143 F2 individuals derived from a cross UR206 x UR203 were used to construct a linkage map. The resistant gene was mapped on the chromosome 7AL based on the mapped microsatellite makers. The map spanned 52.1 cM and the order of these microsatellite loci agreed well with the established microsatellite map of chromosome arm 7AL. The resistance gene was flanked by the microsatellite loci Xwmc273 and Xpsp3003, with the genetic distances of 2.2 cM and 3.8 cM, respectively. On the basis of the origin and chromosomal location of the gene, it was temporarily designated PmU.
authors.affiliation: Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, 100081 Beijing, People's Republic of China.
authors.forename: Y C
authors.initials: YC
authors.lastname: Qiu
authors.forename: R H
authors.initials: RH
authors.lastname: Zhou
authors.forename: X Y
authors.initials: XY
authors.lastname: Kong
authors.forename: S S
authors.initials: SS
authors.lastname: Zhang
authors.forename: J Z
authors.initials: JZ
authors.lastname: Jia
mesh_terms: D001203:Ascomycota; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018014:Gene Transfer Techniques; D017343:Genes, Plant / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D014908:Triticum / Q000235:genetics*