title: Characterization of the human MDR1 gene.
abstract: P-glycoprotein (Pgp), an ATP-dependent efflux transporter that protects the body from environmental toxins and xenobiotics, is encoded by the human MDR1 gene. Human MDR1 is located on chromosomal region 7q21. Although several different single nucleotide polymorphisms were shown to influence Pgp expression and activity, the reported length of the MDR1 gene in Genbank and other databases continues to evolve and varies between 6.3 kilobases (kb) and 210 kb. With DNA derived from human cell lines and tissues, we have characterized the MDR1 genomic sequence to be 209 kb.
authors.affiliation: Department of Pharmaceutics, University of Washington, Seattle, Washington 98195-7610, USA.
authors.forename: Miklos
authors.initials: M
authors.lastname: Bodor
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Kelly
authors.forename: Rodney J
authors.initials: RJ
authors.lastname: Ho
mesh_terms: D015139:Blotting, Southern; D045744:Cell Line, Tumor / Q000737:chemistry; D004247:DNA / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D019450:Genes, MDR; D006801:Humans; D007438:Introns; D008099:Liver / Q000737:chemistry; D008214:Lymphocytes / Q000737:chemistry; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA