title: Rapid identification of Lactobacillus acidophilus by restriction analysis of the 16S-23S rRNA intergenic spacer region and flanking 23S rRNA gene.
abstract: A rapid molecular approach was developed for the initial identification of Lactobacillus acidophilus strains which are difficult to identify using a single biochemical test. The 16S-23S rRNA intergenic spacer regions and flanking 23S rRNA genes of 19 strains of lactobacilli were amplified and the nucleotide sequences and restriction site polymorphisms were analyzed. AluI was the most useful of the restriction enzymes analyzed and produced reproducible digestion profiles in the L. helveticus, L. plantarum, and L. casei groups, as well as in L. acidophilus. This restriction fragment length polymorphism method may be useful for the identification of L. acidophilus strains in dairy products.
authors.affiliation: Division of Food Science, Korea University, 136-701, Seoul, Korea.
authors.forename: Kyoung-Sik
authors.initials: KS
authors.lastname: Han
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Kim
authors.forename: Seungkook
authors.initials: S
authors.lastname: Choi
authors.forename: Sejong
authors.initials: S
authors.lastname: Oh
authors.forename: Sungsu
authors.initials: S
authors.lastname: Park
authors.forename: Sae-Hun
authors.initials: SH
authors.lastname: Kim
authors.forename: Kwang-Youn
authors.initials: KY
authors.lastname: Whang
mesh_terms: D024509:3' Flanking Region / Q000235:genetics; D015169:Colony Count, Microbial / Q000379:methods*; D021901:DNA, Intergenic / Q000235:genetics; D007779:Lactobacillus acidophilus / Q000235:genetics* / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D015183:Restriction Mapping / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D021901:DNA, Intergenic; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S