Numerically simulated pH-induced reactivation of catalytic activity of horseradish peroxidase.
A model mechanism that accounts for the experimentally observed pH-dependent enhancement of enzymatic activity of horseradish peroxidase through the catalase-like pathway is proposed. Predictions of the model are tested against a number of experimental results to confirm that kinetic constants used in the numerical simulation are correctly chosen and that the model can be used to emulate the reaction between horseradish peroxidase and hydrogen peroxide in a wide range of conditions.
Popovic-Bijelić
Ana
A
Faculty of Physical Chemistry, University of Belgrade, Serbia and Montenegro. vladana.vukojevic@cmm.ki.se
Bijelić
Goran
G
Kolar-Anić
Ljiljana
L
Vukojević
Vladana
V
D002384:Catalysis; D003198:Computer Simulation; D004789:Enzyme Activation / Q000187:drug effects*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D013053:Spectrophotometry
D016877:Oxidants; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase