Human immunodeficiency virus type 1 gp120 and other activation stimuli are highly effective in triggering alpha interferon and CC chemokine production in circulating plasmacytoid but not myeloid dendritic cells.
Exposure to aldrithiol-2-inactivated human immunodeficiency virus type 1 or gp120, but not gp41, triggered alpha interferon (IFN-alpha), CC chemokine ligand 2 (CCL2), CCL3, and CCL4 production in human plasmacytoid dendritic cells (DCs) but not in myeloid DCs (M-DCs) or monocyte-derived DCs from the same donors. The nonresponsiveness of M-DCs for IFN-alpha/beta production was a general feature specific to these cells, as they also failed to produce it in response to inactivated influenza virus, poly(I-C), lipopolysaccharide, Staphylococcus aureus Cowans I, or CD40L. The different capacities of circulating DC subsets to produce immune mediators in response to most stimuli argue for a different role for these cells in the regulation of innate immunity to pathogens.
Del Cornò
Manuela
M
Istituto Superiore di Sanità, Department of Cell Biology and Neurosciences, Viale Regina Elena 299, 00161 Rome, Italy.
Gauzzi
Maria Cristina
MC
Penna
Giuseppe
G
Belardelli
Filippo
F
Adorini
Luciano
L
Gessani
Sandra
S
D023201:CD40 Ligand / Q000276:immunology; D018932:Chemokine CCL2 / Q000096:biosynthesis; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019742:Chemokines, CC / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000276:immunology; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis; D009975:Orthomyxoviridae / Q000276:immunology; D011070:Poly I-C / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology
C111835:CCL2 protein, human; C066507:CCL4 protein, human; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019742:Chemokines, CC; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D016898:Interferon-alpha; D008070:Lipopolysaccharides; D019402:Macrophage Inflammatory Proteins; D023201:CD40 Ligand; D011070:Poly I-C