Differential modulatory effect of NGF on MHC class I and class II expression in spinal cord cells of EAE rats.
Nerve growth factor (NGF) undergoes significant changes in the central nervous system (CNS) of patients affected by multiple sclerosis (MS) and of rats with experimental allergic encephalomyelitis (EAE). The major histocompatibility complex (MCH) class I and class II antigens are molecules that play a pivotal role in these neuro-inflammatory disorders. The aim of this study was to investigate the role of NGF on MCH class I and class II antigens in spinal cords cells of EAE rats. It was found that the administration of NGF in EAE rats enhances MHC-I, IFN-gamma receptor and interferon regulatory factor-1 expression on the neurons but not in the glial cells, while NGF decreased MHC class II antigen in the glial cells. NGF administration into the brain of EAE rats has no effect on TNF-alpha expression. The present findings suggest that NGF may have a regulatory function in spinal cord cells during tissue inflammation.
Stampachiacchiere
Barbara
B
Institute of Neurobiology and Molecular Medicine, Department of Neurobiology, National Research Council (CNR), Italy.
Aloe
Luigi
L
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002452:Cell Count / Q000379:methods; D045304:Cytochromes c / Q000008:administration & dosage; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D050856:Interferon Regulatory Factor-1 / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D053583:Interleukin-1beta; D020932:Nerve Growth Factor / Q000008:administration & dosage* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects*; D010446:Peptide Fragments / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D017471:Receptors, Interferon / Q000378:metabolism; D013116:Spinal Cord / Q000473:pathology*; D000096743:Interferon gamma Receptor
D045304:Cytochromes c; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D050856:Interferon Regulatory Factor-1; D007375:Interleukin-1; D016753:Interleukin-10; D053583:Interleukin-1beta; D020932:Nerve Growth Factor; D010446:Peptide Fragments; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor; C052994:interleukin-1beta (163-171)