title: The complete mitochondrial genome of the rayfish Raja porosa (Chondrichthyes, Rajidae).
abstract: We isolated mitochondrial DNA from the rayfish Raja porosa by long-polymerase chain reaction (Long-PCR) with conserved primers, and sequenced it by primer walking method using flanking sequences as sequencing primers. R. porosa mitochondrial DNA consists of 16,972 bp and its structural organization is conserved in comparison with other fishes and mammals. Based on the mitochondrial cytochrome b (cyt b) sequence, the phylogenetic position of R. porosa among cartilaginous fishes was inferred using different phylogenetic methods (ML-based quartet puzzling, Neighbor-joining (NJ) and Bayesian approaches). In this paper, we report the characteristics of the R. porosa mitochondrial genome including structural organization, base composition of rRNAs, tRNAs and protein-encoding genes and characteristics of mitochondrial tRNAs. These findings are applicable to comparative mitogenomics of R. porosa with other related taxa.
authors.affiliation: Polar BioCenter, Korea Polar Research Institute, Korea Ocean Research & Development Institute, Ansan, South Korea.
authors.forename: Il-Chan
authors.initials: IC
authors.lastname: Kim
authors.forename: Sang-Oun
authors.initials: SO
authors.lastname: Jung
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Chang Joo
authors.initials: CJ
authors.lastname: Lee
authors.forename: Joong-Ki
authors.initials: JK
authors.lastname: Park
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D003062:Codon; D045303:Cytochromes b / Q000235:genetics; D004247:DNA / Q000378:metabolism; D017931:DNA Primers; D004272:DNA, Mitochondrial; D016678:Genome; D016013:Likelihood Functions; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000737:chemistry; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012860:Skates, Fish
chemical_list: D003062:Codon; D017931:DNA Primers; D004272:DNA, Mitochondrial; D009711:Nucleotides; D004247:DNA; D045303:Cytochromes b