title: [Study of human smooth muscle cells seeding in biologic blood vessel matrix].
abstract: To explore the human smooth muscle cells seeding in blood vessel of minor pig after trypsin treatment and provide data for xenotransplantation and for using pig vessel in tissue engineering. HE and silver stain were used for checking the smooth muscle cells seeding in acellular blood vessel. The results showed that the smooth muscle cells seeding succeeded and the smooth muscle cells were in normal morphological distribution. These demonstrate that the pig aorta can be used for smooth muscle cells seeding, and hence for constructing new vascular grafts.
authors.affiliation: Department of Anatomy, Biomechanics Section under the Key Lab for Biomechanics & Tissue Engineering of Ministry of Education, The Third Military Medical University, Chongqing, China.
authors.forename: Chuhong
authors.initials: C
authors.lastname: Zhu
authors.forename: Dajun
authors.initials: D
authors.lastname: Ying
authors.forename: Jianhong
authors.initials: J
authors.lastname: Mi
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000166:cytology; D002469:Cell Separation; D017690:Cell Transplantation; D005260:Female; D006801:Humans; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering / Q000379:methods; D014183:Transplantation, Heterologous