New source of genetic polymorphisms in Lepidoptera?
The variability level of the ISSR (inter-simple sequences repeat) primer (GACA)4 was examined in the three Lepidoptera families Pyralidae, Sphingidae and Pieridae. Our study shows that the tetra-repeat (GACA)n is evidently present in sufficient numbers in these butterflies to provide informative DNA fingerprints. The variability is mostly rather high, but within a comparable range to other ISSR studies. Although less polymorphisms may be encountered in some butterfly families, this study indicates that high variability of this marker may be a common characteristic of Lepidoptera genomes. An appeal for a minimal level of standardization of ISSR-PCR data analysis is formulated to enable an exact comparison between the groups of organisms studied with this fingerprint technique.
Hundsdoerfer
Anna K
AK
Institut für Pharmazie und Molekulare Biotechnologie, Abteilung Biologie, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany. anna.hundsdoerfer@snsd.smwk
Wink
Michael
M
D000818:Animals; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D007915:Lepidoptera / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic
D017931:DNA Primers; D004247:DNA