Expansion of family Reoviridae to include nine-segmented dsRNA viruses: isolation and characterization of a new virus designated Aedes pseudoscutellaris reovirus assigned to a proposed genus (Dinovernavirus).
Family Reoviridae is known, by definition, to contain dsRNA viruses with 10-12 genome segments. We report here the characterization of the first member of this family with a nine-segmented genome. This virus was isolated from Aedes pseudoscutellaris mosquito cells and designated aedes pseudoscutellaris reovirus (APRV). Virions are single-shelled with turrets but are non-occluded by contrast to cypoviruses. APRV replicates in various mosquito cell lines, but not in mice or mammalian cells. Complete sequence analysis showed that APRV is phylogenetically related to cypoviruses, fijiviruses and oryzaviruses. The maximum amino acid identities with cypoviruses, oryzaviruses or fijiviruses in the polymerase, are compatible with values observed between these genera and lower than values within a given genus. This suggests that APRV should be classified within a new genus that we designated Dinovernavirus (sigla from D: Double-stranded, i: insect, nove: nine from the latin "novem", rna: RNA, virus) in family Reoviridae.
Attoui
Houssam
H
Unité des Virus Emergents EA3292, Laboratoire de Virologie Moléculaire, Tropicale et Transfusionnelle, Faculté de Médecine de Marseille, 27 Boulevard Jean Moulin, 13005 Marseille cedex 5, France. h-attoui-ets-ap@gulliver.fr
Mohd Jaafar
Fauziah
F
Belhouchet
Mourad
M
Biagini
Philippe
P
Cantaloube
Jean-François
JF
de Micco
Philippe
P
de Lamballerie
Xavier
X
D000330:Aedes / Q000821:virology*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D016679:Genome, Viral; D051379:Mice; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012087:Reoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D013045:Species Specificity; D014779:Virus Replication
D012367:RNA, Viral