title: MCL-1 (myosin light chains-1) in differential diagnosis of dyspnea.
abstract: Myosin light chains-1 (MLC-1) have been recently associated with the markers of heart function (NYHA, LVEF, NT-proBNP). Verification of the relationship between markers of heart function (New York Heart Association classification (NYHA), left ventricle ejection fraction determination (LVEF), N terminal prohormone of natriuretic peptide B type BNP (NT-proBNP) and concentrations of myosin light chains-1 (MLC-1) was assessed. Patients examined for dyspnea without signs of acute coronary syndrome. All patients underwent echocardiography (calculation of left ventricle ejection fraction--LVEF) and in the serum of all subjects NT-proBNP (ELEIA) and MLC-1 (ELISA) were determined. In the 38 patients (21 men, 17 women), mean age of 58 years (+/-12 years as 1 SD), a significant negative correlation was found between NT-proBNP and LVEF (r = - 0.47; p = 0.02, Spearman). The median levels of NT pro-BNP were closely associated with NYHA classification (type II--584 ng/l, type III--2792 ng/l, type IV--6400 ng/l; p < 0.05). Individuals with clinical NYHA IV differed significantly in median MLC-1 concentrations from persons with clinical NYHA classification II and III (type II--5.7 ng/l, type III--8.9 ng/l, type IV--17 ng/l; p < 0.05). A significant negative correlation between MLC-1 and LVEF (-0.35; p < 0.03) and significant positive correlations between MLC-1 and NT-proBNP (0.42; p < 0.012) were found. In conclusion MLC-1 cannot be used as a diagnostic marker in differential diagnosis of dyspnea.
authors.affiliation: Department of Laboratory Medicine, Hospital Sternberk, Czech Republic. david.stejskal@quick.cz
authors.forename: David
authors.initials: D
authors.lastname: Stejskal
authors.forename: Borek
authors.initials: B
authors.lastname: Lacnák
authors.forename: Katerina
authors.initials: K
authors.lastname: Andelová
authors.forename: Marcela
authors.initials: M
authors.lastname: Skvarilová
authors.forename: Josef
authors.initials: J
authors.lastname: Bartek
mesh_terms: D015415:Biomarkers / Q000097:blood; D003937:Diagnosis, Differential; D004417:Dyspnea / Q000209:etiology*; D005260:Female; D006333:Heart Failure / Q000150:complications / Q000175:diagnosis* / Q000503:physiopathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D018994:Myosin Light Chains / Q000097:blood*; D020097:Natriuretic Peptide, Brain / Q000097:blood; D010446:Peptide Fragments / Q000097:blood; D012372:ROC Curve; D012680:Sensitivity and Specificity; D013318:Stroke Volume
chemical_list: D015415:Biomarkers; D018994:Myosin Light Chains; D010446:Peptide Fragments; C076659:myosin light chain I; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain