IL-10 promoter single nucleotide polymorphisms are significantly associated with resistance to leprosy.
The minor haplotype -3575A/-2849G/-2763C in IL-10 promoter has been defined as a marker of disease resistance to leprosy and its severity in Brazilian population. Our investigation of six single-nucleotide polymorphisms (SNPs) in IL-10 promoter in 282 Indian leprosy patients and 266 healthy controls by direct PCR sequencing, however, showed that the extended haplotype: -3575T/-2849G/-2763C/-1082A/-819C/-592C was associated with resistance to leprosy per se and to the development of severe form of leprosy, using either a binomial (controls vs cases, P=0.01, OR=0.58, CI=0.37-0.89) or ordinal (controls vs paucibacillary vs multibacillary, P=0.004) model. Whereas, IL-10 haplotype -3575T/-2849G/-2763C/-1082A/-819T/-592A was associated with the risk of development of severe form of leprosy (P=0.0002) in contrast to the minor risk haplotype -3575T/-2849A/-2763C in the Brazilian population. The role of IL-10 promoter SNPs in Brazilian and Indian population strongly suggests the involvement of IL-10 locus in the outcome of leprosy.
Malhotra
Dheeraj
D
National Centre of Applied Human Genetics, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Darvishi
Katayoon
K
Sood
Soni
S
Sharma
Swarkar
S
Grover
Chander
C
Relhan
Vineet
V
Reddy
B S N
BS
Bamezai
R N K
RN
D001938:Brazil; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics* / Q000276:immunology; D007199:Indians, South American; D016753:Interleukin-10 / Q000235:genetics*; D007918:Leprosy / Q000235:genetics* / Q000276:immunology; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics
D005819:Genetic Markers; D016753:Interleukin-10