title: Antibody profiles characteristic of Mycobacterium tuberculosis infection state.
abstract: The relationship between specific antibody profiles and tuberculosis (TB) state was investigated by measuring serum antibody levels to six Mycobacterium tuberculosis antigens in human subjects grouped into four diagnostic categories: active disease, inactive (past) tuberculosis, latent infection without radiographic chest abnormalities, and infection free. Statistical data analyses showed that the latter two groups were serologically indistinguishable and that active tuberculosis and inactive tuberculosis were characterized by different antibody profiles. Antibodies to the 38-kDa antigen, alanine dehydrogenase, and Rv2626c were associated with active TB, while antibodies to the 16-kDa antigen, ferredoxin A, and ESAT-6 were associated with inactive TB. Thus, the targets of the immune response vary with tuberculosis state. The correlation between bacterial antigen production and infection stage was investigated in mice infected with M. tuberculosis by bacterial transcription profiling. It was found that levels of transcripts encoding the six M. tuberculosis antigens varied during infection. Together, the data indicate that antigen composition of tubercle bacilli varies with stage of infection and that immunoprofiling can distinguish between tuberculosis states.
authors.affiliation: University of Medicine and Dentistry of New Jersey, Newark, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Davidow
authors.forename: Ganga V
authors.initials: GV
authors.lastname: Kanaujia
authors.forename: Lanbo
authors.initials: L
authors.lastname: Shi
authors.forename: Justin
authors.initials: J
authors.lastname: Kaviar
authors.forename: Xudong
authors.initials: X
authors.lastname: Guo
authors.forename: Nackmoon
authors.initials: N
authors.lastname: Sung
authors.forename: Gilla
authors.initials: G
authors.lastname: Kaplan
authors.forename: Dick
authors.initials: D
authors.lastname: Menzies
authors.forename: Maria L
authors.initials: ML
authors.lastname: Gennaro
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins; D018450:Disease Progression; D005260:Female; D005288:Ferredoxins / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D014158:Transcription, Genetic; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D005288:Ferredoxins; C072681:Mycobacterium tuberculosis antigens