title: The baboon as a good model for studies of human kidney development.
abstract: Because of the improved survival of premature neonates in recent years, it is important to investigate the effects of premature delivery on the kidney, in which nephrogenesis is still ongoing during the third trimester. Hence, an appropriate animal model that is similar to humans is essential. The aim of the current study is to determine the time course of nephrogenesis in the baboon, to establish whether it is a suitable model of human nephrogenesis. At the Southwest Foundation for Biomedical Research (San Antonio, TX), fetal baboons were delivered prematurely by cesarean delivery and at term by natural delivery. Fixed kidneys from 125-, 140-, 175-, and 185-d gestation baboons were assessed morphologically for evidence of a nephrogenic zone. Nephron number, kidney volume, and glomerular and corpuscle volume were also estimated using unbiased stereology. Morphologic assessment confirmed the presence of metanephric mesenchyme and immature glomeruli in the nephrogenic zone of the kidneys from the prematurely delivered fetuses at 125 and 140 d gestation. At 175 d gestation and at term, the nephrons seemed to be mature. Both kidney weight (R2= 0.918, p = 0.0002) and kidney volume (R2= 0.837, p = 0.001) were very strongly correlated with nephron number. There was also a direct relationship between gestational age (R2= 0.589, p = 0.03) and birth weight (R2= 0.562, p = 0.03) with nephron number. In conclusion, in this study, nephrogenesis in the baboon is complete before term by 175 d gestation, which is similar to humans. Hence, the baboon is a suitable model for future studies to investigate human kidney development.
authors.affiliation: Department of Anatomy and Cell Biology, Monash University, Clayton, Victoria, Australia. Lina.Gubbhaju@med.monash.edu.au
authors.forename: Lina
authors.initials: L
authors.lastname: Gubhaju
authors.forename: M Jane
authors.initials: MJ
authors.lastname: Black
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001724:Birth Weight; D007668:Kidney / Q000254:growth & development*; D023421:Models, Animal; D009399:Nephrons / Q000254:growth & development; D009929:Organ Size; D010215:Papio