[Hybrid Capture 2 vs HPV Consensus kit: comparison of 2 assays for human papillomavirus detection and typing].
BACKGROUND
Many laboratories use the DNA Hybrid Capture 2 HPV-high risk assay (Digene) to detect and type oncogenic HPV. The aim of this work was to compare this assay with a new HPV genotyping assay: HPV Consensus kit (Argène). Actually, this assay is not commercially available.


METHODS
Ninety-four cervical samples were tested with both the routine assay Hybrid Capture 2 and the HPV Consensus kit. Discordant results were analysed by amplification with a nested PCR and sequencing of amplified products.


RESULTS
Only 81 results could be analysed concerning the oncogenic risk. The overall concordance was 92,6%. But we find 13 "generic" results with the HPV Consensus kit, the generic probe including high risk and low risk genotypes.


CONCLUSIONS
HPV Consensus kit results showed a better detection sensitivity for this assay than Hybrid Capture 2 assay. Nevertheless, "generic" results give no information about the oncogenic risk of the HPV detected in a sample.
Hantz
S
S
Laboratoire de bactériologie-virologie-hygiène, CHRU Dupuytren, 2 avenue Martin-Luther-King, 87042 Limoges cedex, France. shantz@wanadoo.fr
Caly
H
H
Decroisette
E
E
Fermeaux
V
V
Bakeland
D
D
Pascal
B
B
Darreye
G
G
Dussartre
C
C
Rogez
S
S
Aubard
Y
Y
Denis
F
F
Alain
S
S
D017931:DNA Primers; D005838:Genotype; D006801:Humans; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction
D017931:DNA Primers