title: Anti-thrombotic effect of strawberries.
abstract: Because of the high mortality, prevention of arterial thrombotic disease has top priority in developed countries. As inappropriate diet is known to predispose to acute thrombotic events, regular intake of an anti-thrombotic diet may offer a convenient and effective method of prevention. As part of a systematic investigation into the anti-thrombotic effect of fruits and vegetables, strawberry varieties were tested in this study. An in vitro platelet function test (haemostatometry) was used for screening strawberry filtrates. Those that showed significant antiplatelet effect were further assessed with a laser-induced thrombosis test in mice. Measurement of flow-mediated vasodilation in the femoral artery of mice reflected the effect on the vascular endothelium. Correlation between the effects on platelet reactivity in vitro and the antioxidant activity (hypoxanthine/xanthine oxidase test) or phenolic compound content was assessed. Strawberry varieties KYSt-4 (Nohime), KYSt-11 (Kurume IH-1) and KYSt-17 (Kurume 58) showed significant antiplatelet activity both in vitro and, after oral administration, in vivo. Both KYSt-11 and KYSt-17, but not KYSt-4, significantly reduced flow-mediated vasodilation; that is, caused endothelial dysfunction. Antiplatelet activities were heat stable. Significant correlation was found between antiplatelet and antioxidant activities (P=0.049, R=0.23) or total phenolic compounds (P=0.0096, R=0.36). Of the tested strawberry varieties, KYSt-4, KYSt-11 and KYSt-17 showed significant anti-thrombotic effect. The dual mechanism of the effect may involve a direct inhibition of both platelet function and antioxidant activities.
authors.affiliation: Laboratory of Physiology, Faculty of Nutrition and High Technology Research Centre, Kobe Gakuin University, Kobe, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Naemura
authors.forename: Teruyo
authors.initials: T
authors.lastname: Mitani
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ijiri
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Tamura
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Yamashita
authors.forename: Makoto
authors.initials: M
authors.lastname: Okimura
authors.forename: Junichiro
authors.initials: J
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D001780:Blood Coagulation Tests; D004032:Diet; D031985:Fragaria / Q000145:classification; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010974:Platelet Aggregation; D010975:Platelet Aggregation Inhibitors; D010979:Platelet Function Tests; D051381:Rats; D017208:Rats, Wistar; D013927:Thrombosis / Q000209:etiology / Q000517:prevention & control*
chemical_list: D010975:Platelet Aggregation Inhibitors