Extended sequence and functional analysis of the butirosin biosynthetic gene cluster in Bacillus circulans SANK 72073.
Butirosin produced by Bacillus circulans is among the clinically important 2-deoxystreptamine containing aminoglycoside antibiotics and its unique structure is found in (S)-4-amino-2-hydroxyburyric acid substituted at C-1 of 2-deoxystreptamine. Recently, the key part of the butirosin biosynthetic gene cluster has been identified from Bacillus circulans SANK 72073, however the whole gene for the biosynthesis awaited for identification. In the present study, we undertook extended analysis of the butirosin biosynthetic gene cluster and found nine additional open reading flames (ORFs), btrQ, btrR1, btrR2, btrT, btrU, btrV, btrW, btrX and orf1 in the cluster. In addition, we constructed disruption mutants of btrR1 and btrP-V, and found that the btr genes (ca. 24Kb) between btrR1 and btrP-V are at least required for the butirosin biosynthesis.
Kudo
Fumitaka
F
Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1, Meguro-ku, Tokyo 152-8551, Japan. eguchi@cms.titech.ac.jp
Numakura
Mario
M
Tamegai
Hideyuki
H
Yamamoto
Hideki
H
Eguchi
Tadashi
T
Kakinuma
Katsumi
K
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001407:Bacillus / Q000235:genetics*; D015153:Blotting, Western; D002076:Butirosin Sulfate / Q000096:biosynthesis*; D016386:Chromosome Walking; D002876:Chromosomes, Bacterial / Q000235:genetics; D030541:Databases, Genetic; D005798:Genes, Bacterial / Q000235:genetics*; D005810:Multigene Family / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000235:genetics*; D014158:Transcription, Genetic
D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; C422952:telomerase RNA; D002076:Butirosin Sulfate; D012313:RNA; D019098:Telomerase