title: Cutaneous phaeohyphomycosis due to Alternaria infectoria.
abstract: Here we report a case of cutaneous alternariosis in a 74-year-old man treated by corticotherapy for myasthenia, and presenting with papular, crusted lesions on the left elbow and the right knee. Histological examination of the biopsy specimens showed fungal hyphae associated with round-shaped cells which were highly suggestive of alternariosis. Mycological culture allowed the isolation of a dematiaceous fungus which was identified as a member of the Alternaria infectoria species-group. This was confirmed by PCR amplification and sequencing of the internal transcribed spacer domain of the gene encoding nuclear ribosomal DNA and of the mitochondrial small subunit ribosomal DNA domain. The fungus was therefore referred to the Scientific Institute of Public Health where it was identified as Alternaria infectoria, on the basis of its very small 1 or 2-celled conidia often arranged in long chains and presenting with very long secondary conidiophores. Corticotherapy was stopped and a local antifungal treatment with ketoconazole was initiated, allowing the stabilisation of the cutaneous lesions within 2 months.
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire d'Angers, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Dubois
authors.forename: Marc
authors.initials: M
authors.lastname: Pihet
authors.forename: Christian Le
authors.initials: CL
authors.lastname: Clec'h
authors.forename: Anne
authors.initials: A
authors.lastname: Croué
authors.forename: Hugues
authors.initials: H
authors.lastname: Beguin
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Bouchara
authors.forename: Dominique
authors.initials: D
authors.lastname: Chabasse
mesh_terms: D000328:Adult; D000368:Aged; D000528:Alternaria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D004272:DNA, Mitochondrial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D003881:Dermatomycoses / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology; D005260:Female; D006801:Humans; D007717:Knee / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S