GalNAc-GD1a is localized specifically in ventral spinal roots, but not in dorsal spinal roots.
We investigated the localization of GalNAc-GD1a biochemically in the human and bovine peripheral nervous system (PNS). The high-performance thin-layer chromatography (HPTLC)-overlay method with rabbit IgG polyclonal antibody against GalNAc-GD1a (anti-GalNAc-GD1a antibody) revealed expression of GalNAc-GD1a in the ventral spinal nerve roots (VRs) but not in the dorsal spinal nerve roots (DRs) of both species. The amount of GalNAc-GD1a in the human and bovine VRs was 2.22 +/- 0.35 microg/g wet tissue and 7.71 +/- 0.49 microg/g wet tissue, respectively. These results suggest that IgG anti-GalNAc-GD1a antibody may be involved in disturbance of peripheral motor nerves and in the pathogenesis of pure motor neuropathy.
Yoshino
Hiide
H
Department of Neurology, Kohnodai Hospital, National Center of Neurology and Psychiatry, 1-7-1 Kohnodai, Ichikawa, Chiba 2720-8516, Japan.
Utsunomiya
Iku
I
Taguchi
Kyoji
K
Ariga
Toshio
T
Nagaoka
Takumi
T
Aoyagi
Hiroyuki
H
Asano
Atsuko
A
Yamada
Mitsunori
M
Miyatake
Tadashi
T
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005732:Gangliosides / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D013126:Spinal Nerve Roots / Q000276:immunology* / Q000378:metabolism*
D005732:Gangliosides; D007074:Immunoglobulin G; C055253:IV(4)-galactosyl-N-acetylganglioside GD1a