title: Cell death and cell proliferation in mouse submandibular gland during early post-irradiation phase.
abstract: The effects of irradiation on different cell compartments in the submandibular gland were analyzed in adult C57BL/6 mice exposed to X-ray irradiation and followed up for 10 days. Apoptosis was quantified using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end labeling method (TUNEL). Cell proliferation was detected using immunohistochemistry for proliferating cell nuclear antigen (PCNA). Radiation-induced apoptosis occurred rapidly, reaching a maximum 3 days post-irradiation. The percentage of apoptotic cells increased with the irradiation dose. At day 1 post-irradiation, cell proliferation was significantly reduced in comparison to sham-irradiated controls. After post-irradiation arrest of the cell cycle, proliferation increased in all gland compartments, reaching a maximum at day 6 post-irradiation. The proliferation response corresponded to the dose of irradiation. We suggest that the reason for gland dysfunction could be the coexistence of high apoptotic and proliferative activity in the irradiated gland.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, University of Rijeka, Croatia. bralic_marina@yahoo.com
authors.forename: Marina
authors.initials: M
authors.lastname: Bralic
authors.forename: Miranda
authors.initials: M
authors.lastname: Muhvic-Urek
authors.forename: Valter
authors.initials: V
authors.lastname: Stemberga
authors.forename: Mijo
authors.initials: M
authors.lastname: Golemac
authors.forename: Slaven
authors.initials: S
authors.lastname: Jurkovic
authors.forename: Josipa
authors.initials: J
authors.lastname: Borcic
authors.forename: Alen
authors.initials: A
authors.lastname: Braut
authors.forename: Jelena
authors.initials: J
authors.lastname: Tomac
mesh_terms: D000818:Animals; D001835:Body Weight; D002453:Cell Cycle / Q000528:radiation effects; D016923:Cell Death / Q000528:radiation effects*; D049109:Cell Proliferation / Q000528:radiation effects*; D020287:In Situ Nick-End Labeling; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D013363:Submandibular Gland / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D014965:X-Rays