Genome-wide responses to DNA-damaging agents.
Genome-wide studies of mRNA regulation and phenotypic responses have shown that eukaryotic cells mount a robust and multifaceted response upon exposure to DNA-damaging agents. The integration of theses studies over frameworks provided by protein-protein interactions, protein-DNA interactions, and subcellular localization information have led to the identification of networked responses to damage. Taken together, these studies illustrate that cellular protection from DNA and other macromolecular damage involves an intricate network of proteins involved in many different cellular functions, some of them expected (e.g., DNA repair and cell cycle checkpoints) but many of them unexpected (e.g., protein trafficking and degradation). This review highlights many of the studies that detail genome-wide responses to DNA-damaging agents and examines how these datasets have been used to build a systems view of cellular responses to damage.
Fry
Rebecca C
RC
Biological Engineering Division and Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. rfry@mit.edu
Begley
Thomas J
TJ
Samson
Leona D
LD
D004249:DNA Damage; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics*; D005057:Eukaryotic Cells; D005786:Gene Expression Regulation; D016678:Genome; D016680:Genome, Bacterial; D016681:Genome, Fungal; D013014:SOS Response, Genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*