Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens.
We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.
Hesselink
A T
AT
Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.
van den Brule
A J C
AJ
Groothuismink
Z M A
ZM
Molano
M
M
Berkhof
J
J
Meijer
C J L M
CJ
Snijders
P J F
PJ
D002584:Cervix Uteri / Q000821:virology*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase; D014626:Vaginal Smears
D015344:DNA Probes, HPV; D004279:DNA, Viral; D019914:Taq Polymerase