[Study the effects of flavonoids of Rhizoma Drynariae on gene level of rats without ovaries by technology of cDNA array].
OBJECTIVE
To study the effects of flavonoids of Rhizoma Drynariae on gene level of rats without ovaries (OP) by technology of cDNA array.


METHODS
The models of rats without ovaries were made After. 24 weeks of treatment, put them to death and sampled 6 ml blood from the abdominal aorta and 4cm spinal cord. Take out the total RNA, then separate the mRNA to check.


RESULTS
There is 70 genes difference between the blank control group and model group, 9 genes difference between the blank control group and flavonoids of Rhizoma Drynariae group. The genes over expression of OP rats models regain normal after the models were fed on flavonoids of Rhizoma Drynariae. There are no differences in the spinal cords genes in the cDNA array analysis of this trail.


CONCLUSIONS
The genes over expression of OP rats models regain normal after the models were fed on flavonoids of Rhizoma Drynariae for 6 months. The result shows that the flavonoids of Rhizoma Drynariae does have certain effects on the gene expression of rats without ovaries.
Xie
Yan-Ming
YM
Xiyuan Hospital, Chinese Academy of Traditional Chinese Medicine, Beijing 100091, China.
Qin
Lin-Lin
LL
Chen
Jin-Biao
JB
Cui
Tian-Hong
TH
Zhang
Wen-Jun
WJ
D000818:Animals; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis; D010052:Ovariectomy; D010946:Plants, Medicinal / Q000737:chemistry; D029621:Polypodiaceae / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D005419:Flavonoids