Neural stem cells suppress the hearing threshold shift caused by cochlear ischemia.
Neural stem cells are multipotent progenitor cells that show self-renewal activity. In this study, we assessed the use of neural stem cells for ameliorating ischemia-reperfusion injury of the gerbil cochlea. Neural stem cells were injected into one inner ear through the round window 1 day after ischemic insult. Immunostaining for nestin showed that the distribution of neural stem cells was concentrated within the organ of Corti. Seven days after ischemia, the injury-induced auditory brainstem response threshold shift and progressive inner hair cell damage were markedly less on the neural stem cell-transplanted side. These results suggest that the transplantation of neural stem cells is therapeutically useful for preventing damage to hair cells that occurs after transient ischemia of the cochlea.
Hakuba
Nobuhiro
N
Department of Otolaryngology, Division of Functional Histology, Ehime University School of Medicine, Toon, Ehime, Japan. hakubax@m.ehime-u.ac.jp
Hata
Ryuji
R
Morizane
Isamu
I
Feng
Gu
G
Shimizu
Yoshitaka
Y
Fujita
Kensuke
K
Yoshida
Tadashi
T
Sakanaka
Masahiro
M
Gyo
Kiyofumi
K
D000818:Animals; D001309:Auditory Threshold / Q000502:physiology*; D003051:Cochlea / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery / Q000648:ultrastructure; D004195:Disease Models, Animal; D004427:Ear Diseases / Q000503:physiopathology*; D004622:Embryo, Mammalian; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology / Q000528:radiation effects; D005849:Gerbillinae; D006199:Hair Cells, Auditory, Inner / Q000378:metabolism / Q000503:physiopathology / Q000648:ultrastructure; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D007511:Ischemia / Q000601:surgery*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin