DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis.
In recent years, several plant species have been used as bioindicators, and several tests have been developed to evaluate the toxicity of environmental contaminants on vegetal organisms. In this study, barley (Hordeum vulgare L) seedling was used as bioindicator of cadmium (Cd) pollution in the range of 30-120 mgl(-1). Inhibition of root growth and reduction of total soluble protein content in root tips of barley seedlings were observed with the increase of Cd concentrations. The changes occurring in random amplified polymorphic DNA (RAPD) profiles of root tips following Cd treatment included variation in band intensity, loss of normal bands and appearance of new bands compared with the normal seedlings. Additionally, we found that the effect of changes was dose-dependent. These results indicated that genomic template stability (a qualitative measure reflecting changes in RAPD profiles) was significantly affected at the above Cd concentration. Thus, DNA polymorphisms detected by RAPD analysis could be used as an investigation tool for environmental toxicology and as a useful biomarker assay for the detection of genotoxic effects of Cd pollution on plants.
Liu
Wan
W
Key laboratory of Terrestrial Ecological Processes, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, PR China. liuwan63@hotmail.com
Li
P J
PJ
Qi
X M
XM
Zhou
Q X
QX
Zheng
L
L
Sun
T H
TH
Yang
Y S
YS
D015415:Biomarkers; D002104:Cadmium / Q000633:toxicity*; D004249:DNA Damage; D001467:Hordeum / Q000235:genetics*; D018517:Plant Roots / Q000254:growth & development; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D036226:Seedlings; D012989:Soil Pollutants / Q000633:toxicity*
D015415:Biomarkers; D012989:Soil Pollutants; D002104:Cadmium