title: N-Ethylmaleimide sensitive factor in the cortex of subjects with schizophrenia and bipolar I disorder.
abstract: N-Ethylmaleimide sensitive factor (NSF) is a presynaptic protein that has been suggested to be differentially expressed in the cortex of schizophrenic subjects through both high-throughput proteomic and genomic screening studies. Thus, to expand upon these studies we measured NSF using Western blotting in four regions of the cortex (BA9, 10, 40 and 46), in a cohort comprising 20 schizophrenic subjects, 8 bipolar I disorder subjects, and 20 control subjects. There was no significant difference in NSF levels between diagnostic cohorts in any of the four cortical regions. These findings highlight the importance of validating findings from high-throughput screening studies and do not support changes in cortical NSF as being of significance in schizophrenia or bipolar 1 disorder.
authors.affiliation: The Rebecca L. Cooper Research Laboratories, The Mental Health Research Institute of Victoria, 155 Oak St, Parkville, Victoria 3052, Australia. lgray@mhri.edu.au
authors.forename: Laura
authors.initials: L
authors.lastname: Gray
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Scarr
authors.forename: Brian
authors.initials: B
authors.lastname: Dean
mesh_terms: D000328:Adult; D000368:Aged; D001714:Bipolar Disorder / Q000378:metabolism*; D002102:Cadaver; D002540:Cerebral Cortex / Q000378:metabolism*; D015331:Cohort Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D012559:Schizophrenia / Q000378:metabolism*; D014018:Tissue Distribution