title: Assessment of heparin binding to the AN69 ST hemodialysis membrane: I. Preclinical studies.
abstract: The AN69 ST membrane was designed to render the surface of the native polyacrylonitrile polymer less cationic. This was achieved by layering the membrane with the polycationic biopolymer polyethyleneimine. This new membrane is able to bind heparin to its surface, through electrical interactions, without altering the reactivity of the sulfonate groups of the membrane, regularly distributed in the membrane bulk. The kinetics of unfractionated or low-molecular-weight heparins were studied in vitro and in vivo in sheep. Encouraging results were obtained indicating that heparin-coated hemodialyzers are potent anticoagulants. Priming the AN69 ST membrane-equipped hemodialyzer with heparin, as in regular hemodialysis, could allow drastic reduction of heparin consumption in hemodialysis.
authors.affiliation: Service de Néphrologie, Centre Hospitalier et Universitaire and CNRS FRE 2534 Reims, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Chanard
authors.forename: Sylvie
authors.initials: S
authors.lastname: Lavaud
authors.forename: Bruno
authors.initials: B
authors.lastname: Paris
authors.forename: Fatouma
authors.initials: F
authors.lastname: Toure
authors.forename: Philippe
authors.initials: P
authors.lastname: Rieu
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Renaux
authors.forename: Michel
authors.initials: M
authors.lastname: Thomas
mesh_terms: D000180:Acrylic Resins; D000327:Adsorption; D000706:Anaphylatoxins / Q000378:metabolism; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D000990:Antithrombin III / Q000378:metabolism; D001672:Biocompatible Materials; D003124:Colorimetry; D003167:Complement Activation; D003176:Complement C3 / Q000096:biosynthesis; D016207:Cytokines / Q000145:classification / Q000493:pharmacokinetics; D017985:Dalteparin / Q000494:pharmacology; D017984:Enoxaparin / Q000494:pharmacology; D005112:Extracorporeal Circulation; D015951:Factor Xa / Q000378:metabolism; D065427:Factor Xa Inhibitors; D006493:Heparin / Q000378:metabolism*; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D007700:Kinetics; D008567:Membranes, Artificial; D017762:Nadroparin / Q000494:pharmacology; D010314:Partial Thromboplastin Time; D011094:Polyethyleneimine; D011485:Protein Binding; D006435:Renal Dialysis / Q000295:instrumentation* / Q000379:methods; D012756:Sheep
chemical_list: D000180:Acrylic Resins; D000706:Anaphylatoxins; D000925:Anticoagulants; D001672:Biocompatible Materials; D003176:Complement C3; D016207:Cytokines; D017984:Enoxaparin; D065427:Factor Xa Inhibitors; D006495:Heparin, Low-Molecular-Weight; D008567:Membranes, Artificial; D017762:Nadroparin; C010504:polyacrylonitrile; D000990:Antithrombin III; D011094:Polyethyleneimine; D006493:Heparin; D015951:Factor Xa; D017985:Dalteparin