title: [Expression of mRNA of matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase after cardiopulmonary resuscitation for asphyxial cardiac arrest in rat].
abstract: OBJECTIVE
  To study the change in mRNA expression of matrix metalloproteinase (MMPs) and tissue inhibitor-1 of matrix metalloproteinase (TIMP-1) in brain after cardiopulmonary resuscitation (CPR) for asphyxial cardiac arrest in rat.
  
  
  METHODS
  The animal model of cardiac arrest was reproduced by clamping endotracheal tube at the expiration. Eighty male SD rats were randomly divided into two groups: control group and resuscitation group, and they were again divided into 0, 0.5, 3, 6, 9 hours subgroups (n=8). Evans blue content and mRNA expressions of MMPs and TIMP-1 in the brain after CPR were determined respectively.
  
  
  RESULTS
  The mRNA expression of MMP-9 and TIMP-1 was up-regulated 3 hours after restoration of spontaneous circulation (ROSC). At 6 hours after ROSC, they were markedly high, and the ratio of MMP-9/TIMP-1 was higher too. The mRNA expression of MMP-2 showed no significant change at 9 hours after ROSC.
  
  
  CONCLUSIONS
  The mRNA expression of MMP-9 and TIMP-1 and MMP-9/TIMP-1 are increased at early stage after CPR, but the mRNA expression of MMP-2 shows no significant change.
authors.affiliation: Department of Emergency, The First Hospital Affiliated to Wenzhou Medical College, Wenzhou 325000, Zhejiang, China.
authors.forename: Zhang-ping
authors.initials: ZP
authors.lastname: Li
authors.forename: Shou-quan
authors.initials: SQ
authors.lastname: Chen
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Wang
authors.forename: Wei-jia
authors.initials: WJ
authors.lastname: Huang
authors.forename: Jun-yan
authors.initials: JY
authors.lastname: Cheng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Wan-tie
authors.initials: WT
authors.lastname: Wang
authors.forename: Ying-xia
authors.initials: YX
authors.lastname: Tan
mesh_terms: D000818:Animals; D016887:Cardiopulmonary Resuscitation; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D020780:Matrix Metalloproteinase 9