Determination of pantothenic acid in foods by optical biosensor immunoassay.
An optical biosensor inhibition immunoassay was developed using a specific pantothenic acid-binding protein for the quantitation of free pantothenic acid (vitamin B5) in foodstuffs. Samples were prepared by a simple extraction procedure in buffer, and vitamin content was estimated against authentic calibrants in the same buffer. Performance parameters included a working range of 10-5000 ng/mL, a limit of detection of 4.4 ng/mL, precision relative standard deviation of 5.4-7.1% over a range of concentrations, and recoveries > 95% in the matrixes tested. A wide range of foodstuffs, including National Institute of Standards and Technology reference samples, were tested in 3 independent laboratories and the results were compared with microbiological assay and liquid chromatography/mass spectrometry (LC/MS) methods. The results indicate that the biosensor technique is appropriate for the estimation of pantothenic acid in a wide range of foodstuffs.
Haughey
Simon A
SA
Xenosense Ltd, Unit 18, The Innovation Centre, Northern Ireland Science Park, Queen's Rd, Queen's Island, Belfast, N. Ireland, BT3 9DT. simon.haughey@xenosense.co.uk
O'Kane
Anthony A
AA
Baxter
G Andrew
GA
Kalman
Andras
A
Trisconi
Marie-José
MJ
Indyk
Harvey E
HE
Watene
Gaylene A
GA
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods; D019587:Dietary Supplements / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D005504:Food Analysis; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D041943:Infant Formula / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D010205:Pantothenic Acid / Q000032:analysis*; D020349:Surface Plasmon Resonance
D010205:Pantothenic Acid