title: Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis.
abstract: Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3-XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A-dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany. kinoshita@mpi-cbg.de
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Kinoshita
authors.forename: Tim L
authors.initials: TL
authors.lastname: Noetzel
authors.forename: Laurence
authors.initials: L
authors.lastname: Pelletier
authors.forename: Karl
authors.initials: K
authors.lastname: Mechtler
authors.forename: David N
authors.initials: DN
authors.lastname: Drechsel
authors.forename: Anne
authors.initials: A
authors.lastname: Schwager
authors.forename: Mike
authors.initials: M
authors.lastname: Lee
authors.forename: Jordan W
authors.initials: JW
authors.lastname: Raff
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Hyman
mesh_terms: D000818:Animals; D064127:Aurora Kinases; D018797:Cell Cycle Proteins / Q000502:physiology*; D002457:Cell Extracts; D018385:Centrosome / Q000502:physiology*; D016547:Kinesins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism / Q000502:physiology; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism*; D008938:Mitosis; D009865:Oocytes / Q000737:chemistry; D010766:Phosphorylation; D011494:Protein Kinases / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases; D014157:Transcription Factors / Q000502:physiology*; D029867:Xenopus Proteins / Q000378:metabolism / Q000502:physiology*; D014982:Xenopus laevis
chemical_list: C486738:CKAP5 protein, Xenopus; D018797:Cell Cycle Proteins; D002457:Cell Extracts; C091489:KIF2C protein, human; D008869:Microtubule-Associated Proteins; C474872:TACC3 protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins; D011494:Protein Kinases; C112134:AURKA protein, Xenopus; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases; D016547:Kinesins