Multiplexed protein analysis using antibody microarrays and label-based detection.
This chapter describes methods for the production and use of antibody microarrays. The experimental methods are divided into three sections. The first gives information relating to the preparation and handling of antibodies and the production of microarrays. The second relates to the preparation of the samples, from either blood, tissue, or cell lysates. The third section describes methods for using the samples on antibody microarrays. Two related detection methods are described in which the proteins of a sample to be analyzed are labeled with a tag to allow detection after capture on antibody microarrays.
Haab
Brian B
BB
Laboratory of Cancer Immunodiagnostics, Van Andel Research Institute, Grand Rapids, MI, USA.
D000906:Antibodies / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*
D000906:Antibodies; D011506:Proteins