[Effects of antithrombin-III on cytokines in rats with multiple organ dysfunction syndrome].
OBJECTIVE
To observe the effect of the antithrombin-III (AT-III) on cytokine levels in multiple organ dysfunction syndrome (MODS) in rats.


METHODS
Seventy Wistar rats were randomly divided into three groups: normal control group, MODS group, and AT-III treatment group. The rats in MODS group and the treatment group were given lipopolysaccharide (LPS) two times to replicate systemic inflammatory response syndrome (SIRS)/MODS model. One hour after the injections of LPS, the rats in the treatment group were given AT-III (25 U/kg, 0.5 ml/100 g) intravenously. Four hours after treatment, blood samples were collected in rats. The tissue samples were collected and preserved in formalin. By use of radioimmunoassay and histopathology, the effect of the AT-III was observed in rats with SIRS/MODS.


RESULTS
Endotoxin (ET), interleukin-1 (IL-1), IL-6 and tumor necrosis factor-alpha (TNF-alpha) levels were all increased significantly in MODS animals (all P<0.01), and they were decreased after AT-III treatment (all P<0.01), though still higher than those of the normal controls (all P<0.01). The pathologic changes in lung, kidney and liver in the treatment group were milder than in the MODS group.


CONCLUSIONS
These data suggest that AT-III could inhibit systemic inflammatory response and it might be used in the treatment of SIRS/MODS.
Ren
Xin-sheng
XS
Tianjin Tianhe Hospital, Tianjin 300050, China.
Qiao
You-jie
YJ
Xia
Xin-hua
XH
Xu
Jie
J
Jia
Hong-yan
HY
D000818:Animals; D000990:Antithrombin III / Q000494:pharmacology*; D016207:Cytokines / Q000097:blood*; D004195:Disease Models, Animal; D004731:Endotoxins / Q000097:blood; D007375:Interleukin-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D009102:Multiple Organ Failure / Q000097:blood* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D004731:Endotoxins; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D000990:Antithrombin III