A tightly regulated molecular motor based upon T7 RNA polymerase.
Controlled movement of materials or molecules within the nanometer range is essential in many applications of nanotechnology. Here we report the capture, movement, and release of cargo molecules along DNA by a modified form of T7 RNA polymerase (RNAP) in a manner that is controlled by the sequence of the DNA. Using single-molecule methods, we visualize the assembly and manipulation of nanodevices and the ability to harness rotary and linear forces of the RNAP motor.
Pomerantz
Richard T
RT
Department of Microbiology and Immunology and Graduate Program in Molecular and Cellular Biology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203-2098, USA.
Ramjit
Ravi
R
Gueroui
Zoher
Z
Place
Christophe
C
Anikin
Michael
M
Leuba
Sanford
S
Zlatanova
Jordanka
J
McAllister
William T
WT
D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism; D004800:Enzymes, Immobilized; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology; D012313:RNA / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism
D004800:Enzymes, Immobilized; D020409:Molecular Motor Proteins; D014764:Viral Proteins; D012313:RNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases