Mutator phenotype resulting from DNA polymerase IV overproduction in Escherichia coli: preferential mutagenesis on the lagging strand.
We investigated the mutator effect resulting from overproduction of Escherichia coli DNA polymerase IV. Using lac mutational targets in the two possible orientations on the chromosome, we observed preferential mutagenesis during lagging strand synthesis. The mutator activity likely results from extension of mismatches produced by polymerase III holoenzyme.
Kuban
Wojciech
W
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02106 Warsaw, Poland.
Banach-Orlowska
Magdalena
M
Bialoskorska
Malgorzata
M
Lipowska
Aleksandra
A
Schaaper
Roel M
RM
Jonczyk
Piotr
P
Fijalkowska
Iwona J
IJ
D004258:DNA Polymerase III / Q000235:genetics; D019951:DNA Polymerase beta / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D016296:Mutagenesis / Q000235:genetics*; D010641:Phenotype
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III; D019951:DNA Polymerase beta; C468316:dnaQ protein, E coli