L-Arginine and melatonin interaction in rat intestinal ischemia--reperfusion.
We investigated the combinative effects of L-arginine and melatonin on the contractile responses of terminal ileum after the intestinal ischemia-reperfusion (I/R), in vivo. Male rats were subjected to mesenteric ischemia (30 min) followed by reperfusion (180 min). We have observed a dramatic decrease in spontaneous basal activity and Ach-induced contractile response. Our data clearly showed that the contractility decrease was ameliorated by L-arginine but not by L-NAME. Melatonin has reversed the inhibition of contractility caused by I/R injury in part. We did not observe an augmentation in the contractility of ileum when we use melatonin and L-arginine in combination, in fact, melatonin decreased the protective effect of L-arginine in intestinal I/R injury.
Arslan
S Oktay
SO
Department of Pharmacology, Medical School, Zonguldak Karaelmas University, Zonguldak 67600, Turkey. arslanoktay@yahoo.com
Gelir
Ethem
E
Sayan
Hale
H
Ozacmak
V Haktan
VH
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001120:Arginine / Q000494:pharmacology*; D004357:Drug Synergism; D007082:Ileum / Q000098:blood supply / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D008643:Mesentery / Q000098:blood supply / Q000187:drug effects / Q000503:physiopathology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000098:blood supply / Q000187:drug effects / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology*; D014665:Vasodilator Agents / Q000494:pharmacology
D000927:Anticonvulsants; D014665:Vasodilator Agents; D001120:Arginine; D008550:Melatonin; D000109:Acetylcholine