Extracellular protease of Pseudomonas fluorescens CHA0, a biocontrol factor with activity against the root-knot nematode Meloidogyne incognita.
In Pseudomonas fluorescens CHA0, mutation of the GacA-controlled aprA gene (encoding the major extracellular protease) or the gacA regulatory gene resulted in reduced biocontrol activity against the root-knot nematode Meloidogyne incognita during tomato and soybean infection. Culture supernatants of strain CHA0 inhibited egg hatching and induced mortality of M. incognita juveniles more strongly than did supernatants of aprA and gacA mutants, suggesting that AprA protease contributes to biocontrol.
Siddiqui
Imran Ali
IA
Soil Biology and Ecology Laboratory, Department of Botany, University of Karachi, Karachi-75270, Pakistan.
Haas
Dieter
D
Heeb
Stephan
S
D000818:Animals; D000969:Antinematodal Agents; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020689:Exopeptidases / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D018551:Solanum lycopersicum / Q000469:parasitology; D008969:Molecular Sequence Data; D009154:Mutation; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000469:parasitology; D018517:Plant Roots / Q000469:parasitology*; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000254:growth & development*; D013025:Glycine max / Q000469:parasitology; D014431:Tylenchoidea / Q000254:growth & development* / Q000382:microbiology
D000969:Antinematodal Agents; D001426:Bacterial Proteins; C091100:GacA protein, Bacteria; C449646:AprA protein, Pseudomonas fluorescens; D020689:Exopeptidases