Identification of prognosis markers in pediatric high-risk acute lymphoblastic leukemia.
Gene expression profiling may improve the understanding of the biology behind relapse in pediatric acute lymphoblastic leukemia. Using suppression subtractive hybridization (SSH), cDNA concatenated sequencing (CCS), and reverse transcriptase real-time quantitative polymerase chain reaction (RT-RQ-PCR) on high-risk patient samples with nondeterminant chromosomal translocation, the authors identified 3 genes that were significantly overexpressed in the nonrelapsed patients: the calcium/calmodulin-dependent serine protein kinase (CASK), subunit 2 of the cofactor required for SP1 transcriptional activation (CRSP2), and granzyme K (GZMK). The level of expression of these biomarkers may help identify patients with potentially good prognosis within a group otherwise at high risk of relapse.
Al-Lamki
Zakia
Z
Department of Child Health, Hematology/Oncology Unit, College of Medicine, Sultan Qaboos University, Al-Khod, Oman.
Wali
Yasser A
YA
Wasifuddin
Shah M
SM
Zachariah
Mathew
M
Al-Mjeni
Rayhanah
R
Li
Changping
C
Muralitharan
Shanmugakonar
S
Al-Kharusi
Khalsa
K
Gunaratne
Preethi
P
Peterson
Leif
L
Gibbs
Richard
R
Gingras
Marie-Claude
MC
Margolin
Judith F
JF
D000293:Adolescent; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism*; D011237:Predictive Value of Tests; D011379:Prognosis; D012008:Recurrence; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012307:Risk Factors
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins