[Cell suspension culture and flavonoids production in Saussurea involucrate].
OBJECTIVE
To establish a system of cell suspension culture for Saussurea involucrate.


METHODS
The effects of different factors on cell growth and flavonoids production of S. involucrate were systematically studied including the media, initial pH values of the medium, carbon sources, inoculum quantity, and plant growth substance.


RESULTS
The optimum medium was N6, initial pH values of the medium was 5.8, sucrose concentration was 50 g x L(-1), inoculum quantity was 60-80 g x L(-1) FW. Medium supplemented with BA (0.5 mg x L(-1)) and NAA (3 mg x L(-1)) was suitable for cell growth, but medium containing BA (0.2 mg x L(-1)) and NAA (2 mg x L(-1)) was suitable for flavonoids production.


CONCLUSIONS
Cell growth and flavonoids production in the suspention of S. involucrate culture cell should be optimized.
Wu
Li-qin
LQ
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
Guo
Shun-xing
SX
Xiao
Pei-gen
PG
D002244:Carbon; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media; D005419:Flavonoids / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D030441:Saussurea / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D012639:Seeds / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D013395:Sucrose; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D005419:Flavonoids; D010937:Plant Growth Regulators; D013395:Sucrose; D002244:Carbon