title: Daily rhythms in blood and milk lead toxicokinetics following intravenous administration of lead acetate to dairy cows in summer.
abstract: The objective of this study was to investigate circadian variations of blood and milk lead toxicokinetics in dairy cows in summer. Twenty lactating Holstein animals were randomly assigned to four treatments corresponding to different hours after onset of light (HALO): 2, 8, 14, and 20. Cows received a single intravenous administration of 2.5 mg/kg lead as lead acetate. Blood and milk samples were taken and analyzed by atomic absorption spectrophotometry. For each toxicokinetic parameter, a one-way analysis of variance (ANOVA) was performed to outline the existence of daily variations. Significant blood differences as a function of HALO were found for the hybrid constant of distribution (alpha), hybrid constant of elimination (beta), elimination half-life (t(1/2)beta), area under the curve (AUC), volume of distribution at steady state (V(ss)) and clearance (Cl(B)) (p<0.05). Half-life of elimination presented two peaks at 2 and 14 HALO. Milk data showed significant differences for maximum concentration and AUC (p<0.05). The ratio AUC(milk)/AUC(blood) was utilized to estimate penetration of lead in milk. It differed significantly throughout the day (p<0.05). Milk data for the significant parameters could be fitted to circadian rhythms. No circadian rhythms were detected in blood parameters or in the ratio AUC(milk)/AUC(blood).
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas-EEA Rafaela INTA, Ruta 34, km 227 Rafaela, Santa Fe, Argentina. svaltorta@rafaela.inta.gov.ar
authors.forename: S E
authors.initials: SE
authors.lastname: Valtorta
authors.forename: M C
authors.initials: MC
authors.lastname: Scaglione
authors.forename: P
authors.initials: P
authors.lastname: Acosta
authors.forename: J E
authors.initials: JE
authors.lastname: Coronel
authors.forename: H R
authors.initials: HR
authors.lastname: Beldomenico
authors.forename: J C
authors.initials: JC
authors.lastname: Boggio
mesh_terms: D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002940:Circadian Rhythm; D005260:Female; D007275:Injections, Intravenous; D007774:Lactation / Q000378:metabolism; D007854:Lead / Q000032:analysis* / Q000097:blood; D008892:Milk / Q000737:chemistry*; D009942:Organometallic Compounds / Q000097:blood / Q000493:pharmacokinetics*; D010599:Pharmacokinetics; D012621:Seasons
chemical_list: D009942:Organometallic Compounds; D007854:Lead; C008261:lead acetate