title: Cross-neutralization of the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms by antisera against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom.
abstract: We have previously demonstrated that rabbit antisera raised against crotoxin from Crotalus durissus cascavella venom (cdc-crotoxin) and its PLA2 (cdc-PLA2) neutralized the neurotoxicity of this venom and its crotoxin. In this study, we examined the ability of these antisera to neutralize the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms and their major toxins, cdt-crotoxin and bothropstoxin-I (BthTX-I), respectively, in mouse isolated phrenic nerve-diaphragm preparations. Immunoblotting showed that antiserum to cdc-crotoxin recognized cdt-crotoxin and BthTX-I, while antiserum to cdc-PLA2 recognized cdt-PLA2 and BthTX-I. ELISA corroborated this cross-reactivity. Antiserum to cdc-crotoxin prevented the neuromuscular blockade caused by C. d. terrificus venom and its crotoxin at a venom/crotoxin:antiserum ratio of 1:3. Antiserum to cdc-PLA2 also neutralized the neuromuscular blockade caused by C. d. terrificus venom or its crotoxin at venom or toxin:antiserum ratios of 1:3 and 1:1, respectively. The neuromuscular blockade caused by B. jararacussu venom and BthTX-I was also neutralized by the antisera to cdc-crotoxin and cdc-PLA2 at a venom/toxin:antiserum ratio of 1:10 for both. Commercial equine antivenom raised against C. d. terrificus venom was effective in preventing the neuromuscular blockade typical of B. jararacussu venom (venom:antivenom ratio of 1:2), whereas for BthTX-I the ratio was 1:10. These results show that antiserum produced against PLA2, the major toxin in C. durissus cascavella venom, efficiently neutralized the neurotoxicity of C. d. terrificus and B. jararacussu venoms and their PLA2 toxins.
authors.affiliation: Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, CEP 13083-970 Campinas, SP, Brazil.
authors.forename: Daniela G
authors.initials: DG
authors.lastname: Beghini
authors.forename: Maria Alice
authors.initials: MA
authors.lastname: da Cruz-Höfling
authors.forename: Priscila
authors.initials: P
authors.lastname: Randazzo-Moura
authors.forename: Léa
authors.initials: L
authors.lastname: Rodrigues-Simioni
authors.forename: José Camilo
authors.initials: JC
authors.lastname: Novello
authors.forename: Stephen
authors.initials: S
authors.lastname: Hyslop
authors.forename: Sérgio
authors.initials: S
authors.lastname: Marangoni
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000997:Antivenins / Q000276:immunology* / Q000494:pharmacology; D017837:Bothrops; D002846:Chromatography, Affinity; D003429:Cross Reactions / Q000276:immunology; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000633:toxicity; D017839:Crotalus; D003439:Crotoxin / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology* / Q000494:pharmacology; D015151:Immunoblotting; D009132:Muscles / Q000187:drug effects; D009469:Neuromuscular Junction / Q000187:drug effects; D009498:Neurotoxins / Q000276:immunology / Q000633:toxicity; D009500:Neutralization Tests; D010741:Phospholipases A / Q000276:immunology*; D054467:Phospholipases A2; D011817:Rabbits
chemical_list: D000997:Antivenins; D003435:Crotalid Venoms; D007106:Immune Sera; D009498:Neurotoxins; C078097:bothropstoxin; D003439:Crotoxin; D010741:Phospholipases A; D054467:Phospholipases A2