Facile preparation of carbohydrate microarrays by site-specific, covalent immobilization of unmodified carbohydrates on hydrazide-coated glass slides.
[reaction: see text] A new, simple and efficient immobilization method to attach mono-, di-, oligo-, and polysaccharides to hydrazide-coated glass slides was developed. Protein and cell-binding experiments show that the carbohydrate microarrays prepared by this method are applicable for the rapid analysis of protein-carbohydrate interactions and fast detection of pathogens.
Lee
Myung-Ryul
MR
Department of Chemistry, Yonsei University, Seoul 120-749, Korea.
Shin
Injae
I
D000588:Amines / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods*; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry
D000588:Amines; D002241:Carbohydrates; D006834:Hydrazines; D010100:Oxygen