Desmoplastic fibroma of bone: an immunohistochemical study including beta-catenin expression and mutational analysis for beta-catenin.
Desmoplastic fibroma of bone is a very rare primary bone tumor morphologically resembling desmoid-type fibromatosis, its much more common counterpart of soft tissue. The aim of this study is to investigate the immunohistochemical profile and the involvement of the beta-catenin pathway in desmoplastic fibroma as it is known in desmoid-type fibromatosis. Immunohistochemistry was performed on 13 cases of desmoplastic fibroma for muscle-specific markers, estrogen and progesterone receptors, CD117, beta-catenin, and the potential downstream target of beta-catenin, namely, cyclin D1. In all 13 cases, DNA sequencing was performed for the detection of activating beta-catenin gene mutations. There was no immunoreactivity of CD117, estrogen, and progesterone receptors. Seven cases were immunoreactive for one or more muscle-specific markers. In 6 cases, there was overexpression of beta-catenin in the cytoplasm; in one of these cases, there was also accumulation of beta-catenin in the nucleus. In 6 cases in which DNA sequencing was successful, no beta-catenin mutations were detected. Search in a national database showed that not a single case over a frame of 23 years was associated with occurrence of colon cancer in the same patient. The epidemiological, histological, and immunohistochemical findings in desmoplastic fibroma are suggestive of desmoplastic fibroma being the bony counterpart of the more common desmoid-type fibromatosis of soft tissue. However, the beta-catenin pathway does not seem to have the same essential role in the tumorigenesis of desmoplastic fibroma, as it has in desmoid-type fibromatosis.
Hauben
Esther I
EI
Department of Pathology, Leiden University Medical Centre, 2300 RC Leiden, The Netherlands.
Jundt
Gernot
G
Cleton-Jansen
Anne-Marie
AM
Yavas
Ayse
A
Kroon
Herman M
HM
Van Marck
Eric
E
Hogendoorn
Pancras C W
PC
D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D018220:Fibroma, Desmoplastic / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*; D051176:beta Catenin
C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin