title: Up-regulation of the amino acid transporter ATB(0,+) (SLC6A14) in carcinoma of the cervix.
abstract: OBJECTIVE
  ATB(0,+) is an energy-coupled transporter for arginine and amino acid-based prodrugs. The objective of the study was to examine the expression of this transporter in cervical cancer.
  
  
  METHODS
  Specimens of normal ectocervical mucosa and cervical squamous cell carcinoma were used for determination of ATB(0,+) mRNA levels by RT-PCR. A commercial dot blot of paired normal cervix and cervical cancer cDNA was also used to quantify ATB(0,+) mRNA. ATB(0,+) mRNA and protein in tissue sections were analyzed by in situ hybridization and immunohistochemistry/immunofluorescence.
  
  
  RESULTS
  ATB(0,+) mRNA increased 5.6-fold (P < 0.0004) in cervical cancer compared to normal cervix. This was associated with a parallel increase in ATB(0,+) protein.
  
  
  CONCLUSIONS
  Expression of ATB(0,+) is minimal in normal cervix and is up-regulated in cervical cancer. The up-regulation of this highly concentrative transporter for arginine and prodrugs in cervical cancer has significant clinical and therapeutic relevance.
authors.affiliation: Department of Surgery, Medical College of Georgia, Augusta, GA 30912, USA.
authors.forename: Naren
authors.initials: N
authors.lastname: Gupta
authors.forename: Puttur D
authors.initials: PD
authors.lastname: Prasad
authors.forename: Sharad
authors.initials: S
authors.lastname: Ghamande
authors.forename: Pamela
authors.initials: P
authors.lastname: Moore-Martin
authors.forename: Anne V
authors.initials: AV
authors.lastname: Herdman
authors.forename: Robert G
authors.initials: RG
authors.lastname: Martindale
authors.forename: Robert
authors.initials: R
authors.lastname: Podolsky
authors.forename: Sela
authors.initials: S
authors.lastname: Mager
authors.forename: Malliga E
authors.initials: ME
authors.lastname: Ganapathy
authors.forename: Vadivel
authors.initials: V
authors.lastname: Ganapathy
mesh_terms: D026905:Amino Acid Transport Systems / Q000096:biosynthesis* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: D026905:Amino Acid Transport Systems; D012333:RNA, Messenger; D052247:Nitric Oxide Synthase Type II