Isolation of alpha- and beta-subunits of peak-I hCG and generation of highly specific polyclonal antisera.
Development of polyclonal antisera is still a choice in some hard-pressed budget laboratories. In the present study, an attempt was made to isolate alpha- and beta-subunits from peak-I hCG, generation of polyclonal antisera and their characterization. The anti-hCG-a antisera showed titres of 1: 8000 and anti-hCG-beta antisera 1: 16,000 at 50% binding to radiolabelled hCG in RIA. Studies on specificity using anti hCG-beta antiserum demonstrated no cross-reaction with several hormones tested in the present study, except for hCG-beta and hCG, thus eliciting a highly specific hCG-beta antiserum.
Prasad
Pramod V
PV
Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, Munirka, New Delhi, India.
Chaube
Shail K
SK
Shrivastav
T G
TG
Kumari
G L
GL
Duraiswami
S
S
Muralidhar
K
K
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000276:immunology* / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D015292:Glycoprotein Hormones, alpha Subunit / Q000276:immunology / Q000302:isolation & purification*; D007106:Immune Sera; D011817:Rabbits
D018997:Chorionic Gonadotropin, beta Subunit, Human; D015292:Glycoprotein Hormones, alpha Subunit; D007106:Immune Sera