Allosteric modulation of the nicotinic acetylcholine receptor by physostigmine.
The action of physostigmine on mouse muscle nicotinic acetylcholine receptor expressed in the COS-7 cell line was studied by the patch-clamp technique. Physostigmine accelerated, by allosteric modulation, the rate of desensitization of whole cell currents induced by acetylcholine and decreased the maximal amplitude in concentration-dependent manner.
Svobodová
Lucie
L
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.
Krusek
Jan
J
Hendrych
Tomás
T
Vyskocil
Frantisek
F
D000494:Allosteric Regulation / Q000187:drug effects*; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D010830:Physostigmine / Q000494:pharmacology*; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013997:Time Factors
D002800:Cholinesterase Inhibitors; D011978:Receptors, Nicotinic; D010830:Physostigmine