[Application of green fluorescent protein gene in study of adipocyte differentiation].
OBJECTIVE
To establish a model for dynamic detection of adipocyte differentiation in vivo by green fluorescent protein (GFP).


METHODS
The promoter of peroxisome proliferators-activated receptor gamma 2 (PPARgamma2) gene was amplified by PCR from the genomic DNA of human preadipocytes, inserted into the eukaryotic expression vector pEGFP-1 and transferred into NIH3T3 fibroblasts and preadipocytes which were induced to differentiate into adipocyte.


RESULTS
Upon induction with insulin, dexamethasone and 3-isobutyl-1-methyl xanthine, the transformed human preadipocytes differentiated into mature adipocytes and expressed GFP, but NIH3T3 fibroblasts could not differentiate into adipocytes or express GFP.


CONCLUSIONS
Transformation of human preadipocytes with the recombinant of PPARgamma2 promoter with GFP gene can be employed to dynamically detect the differentiation of adipocytes in vivo.
Zhu
Ji
J
Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China.
Ma
Wen-li
WL
Mao
Xiang-ming
XM
Li
Ling
L
Wu
Qinghua
Q
Zheng
Wen-ling
WL
D017667:Adipocytes / Q000166:cytology*; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D047495:PPAR gamma / Q000235:genetics
D047495:PPAR gamma; D049452:Green Fluorescent Proteins