Analysis of the archaeal sub-seafloor community at Suiyo Seamount on the Izu-Bonin Arc.
A sub-surface archaeal community at the Suiyo Seamount in the Western Pacific Ocean was investigated by 16S rRNA gene sequence and whole-cell in situ hybridization analyses. In this study, we drilled and cased holes at the hydrothermal area of the seamount to minimize contamination of the hydrothermal fluid in the sub-seafloor by penetrating seawater. PCR clone analysis of the hydrothermal fluid samples collected from a cased hole indicated the presence of chemolithoautotrophic primary biomass producers of Archaeoglobales and the Methanococcales-related archaeal HTE1 group, both of which can utilize hydrogen as an electron donor. We discuss the implication of the microbial community on the early history of life and on the search for extraterrestrial life.
Hara
Kurt
K
Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan.
Kakegawa
Takeshi
T
Yamashiro
Kan
K
Maruyama
Akihiko
A
Ishibashi
Jun-Ichiro
J
Marumo
Katsumi
K
Urabe
Tetsuro
T
Yamagishi
Akihiko
A
D001105:Archaea / Q000235:genetics; D019608:Archaeoglobales / Q000235:genetics / Q000302:isolation & purification*; D018533:Biomass; D017753:Ecosystem; D006358:Hot Temperature; D007564:Japan; D017015:Methanococcales / Q000235:genetics / Q000302:isolation & purification*; D010140:Pacific Ocean; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology
D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S