A novel aerobactin utilization cluster in Vibrio vulnificus with a gene involved in the transcription regulation of the iutA homologue.
We demonstrated that Vibrio vulnificus M2799 utilizes aerobactin for growth as an exogenous siderophore under iron-limiting conditions, concomitant with enhanced production of the 76-kDa iron-repressible outer membrane protein. Subsequently, by applying the Fur titration assay method to the M2799 genomic libraries followed by further cloning of the regions surrounding the candidate genes, we identified the 8.4-kb aerobactin utilization gene cluster which consists of five genes arranged in three distinct transcriptional units. It was confirmed by disruption of the corresponding genes that the first unit forming a three-gene operon (vatCDB) and the third unit of a single gene (iutA) encode an ATP-binding cassette transport component and the 76-kDa ferric aerobactin receptor, respectively. The second unit of another single gene (iutR), encodes a homologue of the GntR family of transcriptional repressors. Although transcription of the first and third units was iron-regulated, the iutR gene was transcribed regardless of iron status in the growth medium. Construction of an iutR disruptant coupled with genetic complementation experiments suggested that the gene encodes a transcriptional repressor for iutA. This is the first example of a regulator gene involved in aerobactin-enhanced production of IutA.
Tanabe
Tomotaka
T
Department of Molecular Biopharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 1-1-1 Tsushima-naka, Okayama, Japan.
Naka
Ayaka
A
Aso
Hiroaki
H
Nakao
Hiroshi
H
Narimatsu
Shizuo
S
Inoue
Yuji
Y
Ono
Tomomichi
T
Yamamoto
Shigeo
S
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000222:Adaptation, Physiological / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001692:Biological Transport / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D006877:Hydroxamic Acids / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D009876:Operon; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012097:Repressor Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D041261:Vibrio vulnificus / Q000235:genetics*
D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006877:Hydroxamic Acids; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012097:Repressor Proteins; C034506:aerobactin receptor; C031819:aerobactin; D007501:Iron