title: [Effects of Lycium barbarum polysaccharide on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing mice].
abstract: OBJECTIVE
  To study the effects of Lycium barbarum polysaccharide (LBP) on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing mice and the mechanisms for intervention of tumor immune escape by LBP.
  
  
  METHODS
  H22-bearing mice were given LBP orally for two weeks. T-lymphocyte subsets and the phenotypes of dendritic cells in tumor-infiltrating lymphocytes (TIL) were detected by flow cytometry (FCM).
  
  
  RESULTS
  LBP could significantly increase the numbers of CD4(+) and CD8(+) T cells in TIL as compared with those in model control group (P<0.05). In model control group, the number of dendritic cells in tumor microenvironment decreased markedly, while in LBP-treated group, the increased number of dendritic cells and B7-1 expression were observed, but there were no significant differences between these two groups.
  
  
  CONCLUSIONS
  LBP has anti-tumor effect probably by increasing the numbers of CD4(+) and CD8(+) T cells in TIL to relieve the immunosuppression and enhance the anti-tumor function of the immune system. But whether LBP can recover the phenocyte and function of dendritic cells in H22-bearing mice should be further studied.
authors.affiliation: Department of Pathology, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong Province 510405, China. Yahlihe16@163.com
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: He
authors.forename: Yi
authors.initials: Y
authors.lastname: Ying
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Xu
authors.forename: Jun-Fang
authors.initials: JF
authors.lastname: Su
authors.forename: Hui
authors.initials: H
authors.lastname: Luo
authors.forename: Hui-Feng
authors.initials: HF
authors.lastname: Wang
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D003713:Dendritic Cells / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D008114:Liver Neoplasms, Experimental / Q000276:immunology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008297:Male; D051379:Mice; D016176:T-Lymphocyte Subsets / Q000187:drug effects*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; C500223:lycium barbarum polysaccharide