Use of 2-deoxyglucose in liquid media for the selection of mutant strains of Penicillium echinulatum producing increased cellulase and beta-glucosidase activities.
Mutagenesis and selection were applied to a strain of Penicillium echinulatum by treating conidia with hydrogen peroxide or 1,2,7,8-diepoxyoctane and then by incubating the conidia for 48 h in broth containing microcrystalline cellulose washed in 0.5% (w/v) aqueous 2-deoxyglucose before plating them onto cellulose agar containing 1.5% (w/v) glucose from which colonies showing the fastest production of halos of cellulose hydrolysis were selected. This process resulted in the isolation of two new cellulase-secreting P. echinulatum mutants: strain 9A02S1 showing increased cellulase secretion (2 IU ml-1, measured as filter paper activity) in submerged culture in agitated flasks containing a mineral salts medium and 1% of cellulose, and strain 9A02D1, which proved more suitable for the production of cellulases in semisolid bran culture where it produced 23 IU of beta-glucosidase per gram of wheat bran.
Dillon
Aldo J P
AJ
Institute of Biotechnology, University of Caxias do Sul, 1130 Francisco Getúlio Vargas Street, 95070-560, Caxias do Sul, RS, Brazil. ajpdillo@ucs.br
Zorgi
Cinthia
C
Camassola
Marli
M
Henriques
João Antônio P
JA
D001709:Biotechnology / Q000379:methods; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D003847:Deoxyglucose / Q000378:metabolism*; D016296:Mutagenesis; D009154:Mutation; D010407:Penicillium / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D012641:Selection, Genetic; D015854:Up-Regulation; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D003847:Deoxyglucose; D001617:beta-Glucosidase; D002480:Cellulase