Anti-inflammatory effect of germanium-concentrated yeast against paw oedema is related to the inhibition of arachidonic acid release and prostaglandin E production in RBL 2H3 cells.
1 To investigate anti-inflammatory activity of organic germanium, we measured the effect of germanium-concentrated yeast on arachidonic acid release, prostaglandin E(2) (PGE(2)) production, histamine release, and intracellular H(2)O(2) or hydroperoxide generation in RBL 2H3 cells, and carrageenan-induced paw oedema in rats. 2 Germanium-concentrated yeast dose-dependently inhibited carrageenan-induced paw oedema, suggesting that germanium-concentrated yeast has anti-inflammatory activity in acute inflammation. 3 Germanium-concentrated yeast significantly inhibited melittin-induced arachidonic acid release and PGE(2) production in RBL 2H3 cells. 4 Germanium-concentrated yeast did not affect melittin-induced histamine release and silica-induced intracellular H(2)O(2) or hydroperoxide generation in RBL 2H3 cells. 5 These results suggest that anti-inflammatory activity of germanium-concentrated yeast appears partly to be related to the inhibition of arachidonic acid release and PGE(2) production in RBL 2H3 cells.
Lee
J H
JH
Department of Pathophysiology, College of Pharmacy, Chung-Ang University, 221 Huksuk-dong, Dongjak-gu, Seoul 156-756, South Korea.
Kim
K W
KW
Yoon
M Y
MY
Lee
J Y
JY
Kim
C J
CJ
Sim
S S
SS
D000284:Administration, Oral; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism*; D002351:Carrageenan; D045744:Cell Line, Tumor; D015232:Dinoprostone / Q000096:biosynthesis / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D005857:Germanium / Q000737:chemistry / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D008555:Melitten; D009942:Organometallic Compounds / Q000494:pharmacology*; D051381:Rats; D015003:Yeasts / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D009942:Organometallic Compounds; D005857:Germanium; D008555:Melitten; D016718:Arachidonic Acid; D002351:Carrageenan; D015232:Dinoprostone