title: Isolation and transcriptional analysis of novel tetrachloroethene reductive dehalogenase gene from Desulfitobacterium sp. strain KBC1.
abstract: Strain KBC1, an anaerobic bacterium, that dechlorinates tetrachloroethene (PCE) to trichloroethene was isolated. This strain also dechlorinated high concentrations of PCE at a temperature range of 10 to 40 degrees C and showed high oxygen tolerance. Based on the 16S rRNA gene sequence analysis, this microorganism was identified as a species of the genus Desulfitobacterium. Several species of this genus have been reported to be potent ortho-chlorophenol and PCE dechlorinators; however, the gene coding PCE-specific dehalogenase had not been cloned thus far. In this report, we identified a novel PCE reductive dehalogenase (PrdA) gene from the Desulfitobacterium sp. strain KBC1. These prd genes, including putative membrane anchor protein, were classified as novel type of PCE reductive dehalogenase (approximately 40% homology with the general PCE dehalogenase). It was revealed that the two open reading frames had been transcribed as identical mRNA and were induced strictly in the presence of PCE. This transcriptional regulation appeared to be controlled by the transcriptional activator located downstream of prdAB operon. According to the substrate utility of the strain KBC1 and phylogenetic analysis of PrdA, this microorganism may be expected to play the role of a primary dechlorinator of PCE in the environment.
authors.affiliation: Biotechnology Research Center, KUBOTA Corporation, Osaka, Japan. nori-tu@kubota.co.jp
authors.forename: Norihiko
authors.initials: N
authors.lastname: Tsukagoshi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ezaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Uenaka
authors.forename: Nobukazu
authors.initials: N
authors.lastname: Suzuki
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Kurane
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D045858:Desulfitobacterium / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator / Q000235:genetics; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010100:Oxygen / Q000633:toxicity; D010802:Phylogeny; D021382:Protein Sorting Signals; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D013750:Tetrachloroethylene / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014241:Trichloroethylene / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021382:Protein Sorting Signals; D012336:RNA, Ribosomal, 16S; D014241:Trichloroethylene; D010088:Oxidoreductases; C112990:tetrachloroethene dehalogenase; D010100:Oxygen; D013750:Tetrachloroethylene