title: Detection of aerosolized Alternaria alternata conidia, hyphae, and fragments by using a novel double-immunostaining technique.
abstract: A double-immunostaining halogen immunoassay was developed to identify aerosolized conidia, hyphae, and fragments of Alternaria alternata by using an anti-Alternaria polyclonal antiserum, while, simultaneously, allergy to these components was concurrently determined by using human immunoglobulin E antibodies.
authors.affiliation: Department of Medicine, The University of Sydney, NSW, Australia 2006.
authors.forename: Brett James
authors.initials: BJ
authors.lastname: Green
authors.forename: Detlef
authors.initials: D
authors.lastname: Schmechel
authors.forename: Euan Roger
authors.initials: ER
authors.lastname: Tovey
mesh_terms: D000336:Aerosols; D000528:Alternaria / Q000276:immunology*; D000908:Antibodies, Fungal / Q000276:immunology; D000946:Antigens, Fungal / Q000032:analysis* / Q000276:immunology; D006219:Halogens; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis / Q000276:immunology; D025301:Hyphae / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D007073:Immunoglobulin E / Q000276:immunology; D066298:In Vitro Techniques; D009181:Mycoses / Q000175:diagnosis / Q000276:immunology*
chemical_list: D000336:Aerosols; D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D006219:Halogens; D007073:Immunoglobulin E