Inter-strain variance in late phase of erythematous reaction or leg contracture after local irradiation among three strains of mice.
OBJECTIVE
To gain insights into inter-strain differences in radiosensitivity.


METHODS
Mice of inbred strains, A/J, C57BL/6J, and C3H/HeMs, were irradiated at graded doses ranging from 20 to 60 Gy. Skin reaction and leg contraction were observed for a period of 230 days and between 175 and 350 days, respectively. Gene expressions in leg skin tissue were quantified by quantitative RT-PCR assay at 1, 12 and 72 h after 30 Gy irradiation. Mice were locally irradiated by using a Cs-137 source.


RESULTS
The three strains showed various degrees of susceptibility to irradiation has evaluated by skin scores. Large inter-strain differences were also detected in the lengths of contraction. Expressions of several genes such as Per3 and Rad51ap1 displayed inter-strain differences.


CONCLUSIONS
The continuum model of tissue injury revealed that genetic factor, which varies among strains, is one of the causes of variances in severity of damage after irradiation.
Noda
Shuhei
S
Frontier Research Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba-ken 263-8555, Japan.
Iwakawa
Mayumi
M
Ohta
Toshie
T
Iwata
Masaru
M
Yang
Minfu
M
Goto
Miyako
M
Tanaka
Hiroko
H
Harada
Yoshinobu
Y
Imai
Takashi
T
D000818:Animals; D003286:Contracture / Q000235:genetics / Q000473:pathology*; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D004890:Erythema / Q000235:genetics / Q000473:pathology*; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D035002:Lower Extremity / Q000473:pathology* / Q000528:radiation effects*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D020411:Oligonucleotide Array Sequence Analysis; D011833:Radiation Injuries, Experimental / Q000235:genetics* / Q000473:pathology*; D011836:Radiation Tolerance / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000528:radiation effects; D013997:Time Factors