Human hemarthrosis-derived progenitor cells can differentiate into osteoblast-like cells in vitro.
We hypothesized that intraarticular osteochondral fracture-induced hemarthrosis could be a useful cell source for bone regeneration, as it is thought to contain osteoprogenitor cells derived from bone marrow. Therefore, we investigated whether human hemarthrosis-derived cells have the potential to differentiate into osteoblast-like cells in vitro. We aspirated hemarthrosis from patients suffering from osteochondral fractures of knee joints, and cultured hemarthrosis-derived cells in a medium supplemented with dexamethasone, beta-glycerophosphate, and ascorbic acid, or without them as control. The morphology of the treated cells appeared to be cuboidal shape, differing from spindle-like shape observed in the control. Matrix mineralization was observed only in the treated culture. Alkaline phosphatase activity and gene expression of alkaline phosphatase, parathyroid hormone receptor, osteopontin, and osteocalcin were up-regulated compared with the control. These studies demonstrate that human hemarthrosis-derived cells can differentiate into osteoblast-like cells, i.e., they contain osteoprogenitor cells and are a useful cell source for bone regeneration.
Niikura
Takahiro
T
Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Japan.
Miwa
Masahiko
M
Sakai
Yoshitada
Y
Lee
Sang Yang
SY
Kuroda
Ryosuke
R
Fujishiro
Takaaki
T
Kubo
Seiji
S
Doita
Minoru
M
Kurosaka
Masahiro
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000469:Alkaline Phosphatase / Q000378:metabolism; D001861:Bone Regeneration; D002454:Cell Differentiation; D002478:Cells, Cultured; D002648:Child; D005260:Female; D006395:Hemarthrosis / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D015854:Up-Regulation
D000469:Alkaline Phosphatase