title: Differential modulation of a polymorphism in the COOH terminus of the alpha-subunit of the human epithelial sodium channel by protein kinase Cdelta.
abstract: The A663T polymorphism of the alpha-subunit of the human epithelial sodium channel (hENaC) increases the functional and surface expression of alphabetagamma-hENaC in Xenopus laevis oocytes. The context of this residue in the COOH terminus of alpha-hENaC is important for this effect, as a homologous change in murine ENaC (mENaC), A692T, does not alter functional and surface expression of mENaC. Query of a phosphoprotein database suggested that the alpha-T663 residue might be phosphorylated by PKCdelta. General inhibition of PKC with calphostin C decreased the functional and surface expression of alphaT663-hENaC and not alphaA663-hENaC, and was without effect on alphaA692-mENaC, alphaT692-mENaC, and a chimeric m(1-678)/h(650-669)alphaT663, mbetagamma-ENaC. These data suggest that residues outside of the alpha-hENaC COOH terminus are important for modulation of alphaT663-hENaC trafficking by PKC. In contrast, expression of PKCdelta decreased the functional and surface expression of alphaT663-hENaC and the functional expression of m(1-678)/h(650-669)alphaT663, mbetagamma-ENaC, and was without effect on alphaA663-hENaC, alphaA692-mENaC, or alphaT692-mENaC. PKCdelta did not phosphorylate the COOH terminus of either alphaT663-hENaC or alphaA663-hENaC in vitro, suggesting that it acts indirectly to regulate hENaC trafficking. alphaT663-hENaC was retrieved from the oocyte membrane more slowly than alphaA663-hENaC, and calphostin C increased the rate of alphaT663-hENaC removal from the oocyte membrane to a rate similar to that of alphaA663-hENaC. In contrast, PKCdelta did not alter the rate of removal of alphaT663-hENaC from the oocyte membrane, suggesting that PKCdelta altered rates of alphaT663-hENaC biosynthesis and/or delivery to the plasma membrane. These data are consistent with PKC isoform-specific effects on the intracellular trafficking of alphaT663- vs. alphaA663-hENaC.
authors.affiliation: Renal-Electrolyte Division, Department of Medicine, A919 Scaife Hall, 3550 Terrace St., Pittsburgh, PA 15261, USA.
authors.forename: Wusheng
authors.initials: W
authors.lastname: Yan
authors.forename: Laurence
authors.initials: L
authors.lastname: Suaud
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Kleyman
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Rubenstein
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004553:Electric Conductivity; D053503:Epithelial Sodium Channels; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism* / Q000502:physiology; D010766:Phosphorylation; D011110:Polymorphism, Genetic; D051745:Protein Kinase C-delta / Q000502:physiology*; D021381:Protein Transport; D017386:Sequence Homology, Amino Acid; D015222:Sodium Channels / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection; D014982:Xenopus laevis
chemical_list: D053503:Epithelial Sodium Channels; D015222:Sodium Channels; D051745:Protein Kinase C-delta