Nanostructured ordering of fluorescent markers and single proteins on substrates.
Highly ordered hexagonal nanopatterns of gold clusters on glass substrates were used as anchoring points for the specific attachment of fluorescence dyes and proteins labeled with fluorescence dyes. Thiol- or disulfide-containing linker molecules were used for the binding to the gold dots. In order to ensure specific binding on the gold dots only, the surface area in between the dots was protected against unspecific adsorption. For the attachment of polar low-molecular-weight fluorescence dyes, an octadecyltrichlorosilane self-assembled monolayer was prepared on the surface in between the gold dots, whereas a layer prepared from star-shaped poly(ethylene oxide-stat-propylene oxide) prepolymers was used to prevent unspecific adsorption of proteins between the gold dots. Fluorescence microscopy proved the specific binding of the dyes as well as of the proteins. Scanning force microscopy studies show that each gold dot is only capable of binding one protein at a time.
Groll
Juergen
J
Deutsches Wollforschungsinstitut an der RWTH Aachen e.V. Pauwelsstrasse 8, 52074 Aachen, Germany. groll@dwi.rwth-aachen.de
Albrecht
Krystyna
K
Gasteier
Peter
P
Riethmueller
Silke
S
Ziener
Ulrich
U
Moeller
Martin
M
D001360:Avidin / Q000737:chemistry; D015415:Biomarkers / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
D015415:Biomarkers; D005456:Fluorescent Dyes; D011137:Polystyrenes; D011145:Polyvinyls; D011506:Proteins; D001360:Avidin; D001710:Biotin