title: Expression of HLA-DP0401 molecules for identification of DP0401 restricted antigen specific T cells.
abstract: Human histocompatibility leukocyte antigen (HLA)-DPA1*0103/DPB1*0401 (DP0401) is the most common HLA class II molecule and is present in approximately 45% of the Caucasian population. In this study, soluble HLA-DP0401 molecules were expressed as "empty'' class II molecules in insect cells. Utilizing these soluble DP molecules and the Tetramer Guided Epitope Mapping (TGEM) approach, the influenza A Puerto Rico/8/34 matrix protein (MP) derived peptide MP(41-60) VLMEWLKTRPILSPLTKGIL and the Clostridium tetani Tetanus Toxin (TT) derived peptide TT(634-653) DKISDVSTIVPYIGPALNIV were identified as the DP0401 restricted MP and TT epitopes, respectively. In addition, T cells specific for the cancer testis antigen NY-ESO-1 and the breast/ovarian cancer over-expressing antigen Her-2/neu were detected in DP0401 subjects by DP0401 tetramers. The availability of HLA-DP0401 tetramers should facilitate the study of DP restricted T cell responses.
authors.affiliation: Benaroya Research Institute at Virginia Mason, Seattle, WA, USA.
authors.forename: Junbao
authors.initials: J
authors.lastname: Yang
authors.forename: Laurel
authors.initials: L
authors.lastname: Huston
authors.forename: Deanna
authors.initials: D
authors.lastname: Berger
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Danke
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Liu
authors.forename: Mary L
authors.initials: ML
authors.lastname: Disis
authors.forename: William W
authors.initials: WW
authors.lastname: Kwok
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology; D002460:Cell Line; D004330:Drosophila; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006682:HLA-DP Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D059868:HLA-DP beta-Chains; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013744:Tetanus Toxin / Q000235:genetics / Q000276:immunology; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D018984:Epitopes, T-Lymphocyte; D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D018719:Erb-b2 Receptor Tyrosine Kinases; D013744:Tetanus Toxin; D014763:Viral Matrix Proteins; C505113:HLA-DPB1*04:01 antigen; C488936:M1 protein, Influenza A virus; C504237:peptide NY-ESO-1 157-165