title: Insertion behavior of the Bacillus thuringiensis Cry4Ba insecticidal protein into lipid monolayers.
abstract: Toxicity mechanisms of Bacillus thuringiensis Cry insecticidal proteins involve membrane insertion and lytic pore formation in lipid bilayers of the target larval midgut cell membranes. The B. thuringiensis Cry4Ba mosquito-larvicidal protein has been shown to be capable of permeabilizing liposome vesicles and of forming ion channels in planar lipid bilayers. Here, the membrane interaction of the 65-kDa activated Cry4Ba protein with the lipid monolayers, comprising dipalmitoyl phosphatidylcholine, dioleoyl phosphatidylethanolamine, and cholesterol (Chol), was studied using Langmuir-Blodgett technique. The interactions of the Cry4Ba protein with the lipid monolayers were measured from the surface pressure versus area isotherms of the protein-lipid monolayers. The increase in the mean molecular area was demonstrated as an incorporation of the protein into lipid monolayers. The insertion of the Cry4Ba protein was monitored by measuring as an increase of the surface pressure at constant molecular area. For a given monolayer, the membrane insertion of the Cry4Ba reduced as the initial surface pressure increased. The Cry4Ba protein showed a strong preference of an insertion towards a Chol monolayer. In addition, the mixed monolayers of Chol showed an enhanced effect on the insertion kinetics of Cry4Ba into lipid films, suggesting its involvement in the modulation of the protein insertion. These findings provide the first evidence that the Cry4Ba protein is capable of inserting itself into lipid monolayers, depending on the packing density of the monolayers. Our results also indicate that only a limited part of the protein is likely to be involved in the insertion.
authors.affiliation: Department of Physics, Center of Nanoscience and Nanotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Yodsoi
authors.initials: Y
authors.lastname: Kanintronkul
authors.forename: Toemsak
authors.initials: T
authors.lastname: Srikhirin
authors.forename: Chanan
authors.initials: C
authors.lastname: Angsuthanasombat
authors.forename: Teerakiat
authors.initials: T
authors.lastname: Kerdcharoen
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry* / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001427:Bacterial Toxins / Q000737:chemistry* / Q000378:metabolism; D004731:Endotoxins / Q000737:chemistry* / Q000378:metabolism; D006460:Hemolysin Proteins; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D051381:Rats
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D008563:Membrane Lipids; C030956:insecticidal crystal protein, Bacillus Thuringiensis