title: A human protein-protein interaction network: a resource for annotating the proteome.
abstract: Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a large, highly connected network. Independent pull-down and co-immunoprecipitation assays validated the overall quality of the Y2H interactions. Using topological and GO criteria, a scoring system was developed to define 911 high-confidence interactions among 401 proteins. Furthermore, the network was searched for interactions linking uncharacterized gene products and human disease proteins to regulatory cellular pathways. Two novel Axin-1 interactions were validated experimentally, characterizing ANP32A and CRMP1 as modulators of Wnt signaling. Systematic human protein interaction screens can lead to a more comprehensive understanding of protein function and cellular processes.
authors.affiliation: Max Delbrueck Center for Molecular Medicine, 13092 Berlin-Buch, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Stelzl
authors.forename: Uwe
authors.initials: U
authors.lastname: Worm
authors.forename: Maciej
authors.initials: M
authors.lastname: Lalowski
authors.forename: Christian
authors.initials: C
authors.lastname: Haenig
authors.forename: Felix H
authors.initials: FH
authors.lastname: Brembeck
authors.forename: Heike
authors.initials: H
authors.lastname: Goehler
authors.forename: Martin
authors.initials: M
authors.lastname: Stroedicke
authors.forename: Martina
authors.initials: M
authors.lastname: Zenkner
authors.forename: Anke
authors.initials: A
authors.lastname: Schoenherr
authors.forename: Susanne
authors.initials: S
authors.lastname: Koeppen
authors.forename: Jan
authors.initials: J
authors.lastname: Timm
authors.forename: Sascha
authors.initials: S
authors.lastname: Mintzlaff
authors.forename: Claudia
authors.initials: C
authors.lastname: Abraham
authors.forename: Nicole
authors.initials: N
authors.lastname: Bock
authors.forename: Silvia
authors.initials: S
authors.lastname: Kietzmann
authors.forename: Astrid
authors.initials: A
authors.lastname: Goedde
authors.forename: Engin
authors.initials: E
authors.lastname: Toksöz
authors.forename: Anja
authors.initials: A
authors.lastname: Droege
authors.forename: Sylvia
authors.initials: S
authors.lastname: Krobitsch
authors.forename: Bernhard
authors.initials: B
authors.lastname: Korn
authors.forename: Walter
authors.initials: W
authors.lastname: Birchmeier
authors.forename: Hans
authors.initials: H
authors.lastname: Lehrach
authors.forename: Erich E
authors.initials: EE
authors.lastname: Wanker
mesh_terms: D060466:Axin Protein; D019992:Databases as Topic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008958:Models, Molecular; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009687:Nuclear Proteins; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D040901:Proteomics / Q000379:methods*; D016601:RNA-Binding Proteins; D012097:Repressor Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D000098855:Collapsin Response Mediator Protein 1
chemical_list: D060466:Axin Protein; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; C087425:ANP32A protein, human; C555451:AXIN1 protein, human; C102924:CRMP1 protein, human; D000098855:Collapsin Response Mediator Protein 1