Screening for anabolic steroids in doping analysis by liquid chromatography/electrospray ion trap mass spectrometry.
A fast and selective LC/MS/MS method for the screening of four anabolic steroids in human urine has been developed and validated. Liquid-liquid extraction with diethyl ether was applied after enzymatic hydrolysis. Analyses were performed on an ion trap mass spectrometer equipped with electrospray ionisation. MS/MS was applied for all compounds. The analytical run time was 11 min. The LOD for all compounds varied between 1 and 10 ng/mL. Left-over A samples, which were declared positive by GC/MS for the presence of 3'-hydroxystanozolol, were assessed using the described method.
Deventer
K
K
Ghent University (UGent), Department of Clinical Chemistry, Microbiology and Immunology, Doping Control Laboratory (DoCoLab), Technologiepark 30, B-9052 Zwijnaarde, Belgium. Koen.Deventer@UGent.be
Eenoo
P Van
PV
Delbeke
F T
FT
D045930:Anabolic Agents / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods*; D004300:Doping in Sports; D006801:Humans; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013197:Stanozolol / Q000652:urine; D013256:Steroids / Q000652:urine*; D015813:Substance Abuse Detection / Q000379:methods
D045930:Anabolic Agents; D013256:Steroids; D013197:Stanozolol