Chondroptosis: an immunohistochemical study of apoptosis and Golgi complex in chondrocytes from human osteoarthritic cartilage.
The Golgi complex is thought to play an important role in the apoptotic process of osteoarthritic (OA) chondrocytes. However, the exact relationship between modifications of the Golgi complex and apoptosis in human OA cartilage requires to be established. We compared the patterns and immunolabeling intensities for anti-Golgi 58 K protein with apoptosis markers such as TUNEL and caspase-2L in OA cartilage removed from patients during knee total replacement surgery. We observed important modifications in labeling of the Golgi 58 K protein in OA chondrocytes compared with normal cell. Immunohistochemical analysis revealed co-localization between 58 K protein and caspase-2L, suggesting that this enzyme was localized in Golgi complex of OA chondrocytes. In addition, these cells labeled positive with the TUNEL technique, but in different proportions to caspase-2L. Our results support the concept, previously reported, that apoptosis in OA cartilage (chondroptosis) might be a variant of the classical apoptosis.
Pérez H
E
E
Departamento de Patologìa Experimental, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), México D.F., México.
Luna M
J
J
Rojas M
L
L
Kouri
J B
JB
D000368:Aged; D000642:Ammonia-Lyases / Q000502:physiology; D017209:Apoptosis / Q000502:physiology*; D002358:Cartilage, Articular / Q000473:pathology*; D020169:Caspases / Q000032:analysis; D019902:Chondrocytes / Q000166:cytology*; D005260:Female; D006056:Golgi Apparatus / Q000187:drug effects*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000473:pathology / Q000503:physiopathology*
D020169:Caspases; D000642:Ammonia-Lyases; C500814:Ftcd protein, rat