title: Antibody-based detection of acid-shocked, acid-adapted, and apple juice-incubated Escherichia coli O157:H7.
abstract: Sandwich enzyme-linked immunosorbent assays (sELISA) allow for rapid detection of Escherichia coli (E. coli) O157:H7. Acidic conditions similar to those in certain foods and juices may reduce the ability to detect E. coli O157:H7. Growth of E. coli O157:H7 at pH 4 compared to pH 5-7 reduced fluorescent signal at the lower bacterial concentrations without altering the range of detection. Both acid-adaptation and a subsequent pH 7 incubation reversed sensitivity. Incubation in apple juice was not deleterious to sELISA detection. Exposure to acidic conditions can cause a small reduction in sELISA sensitivity used to detect E. coli O157:H7.
authors.affiliation: Department of Public Health, University of North Florida, 4569 St Johns Bluff Road, Jacksonville, FL 32224, USA. cnyquist@unf.edu
authors.forename: Cynthia
authors.initials: C
authors.lastname: Nyquist-Battie
authors.forename: Leigh
authors.initials: L
authors.lastname: Mathias
authors.forename: Laura E
authors.initials: LE
authors.lastname: Freeman
authors.forename: Deanna
authors.initials: D
authors.lastname: Lund
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Lim
mesh_terms: D000143:Acids / Q000494:pharmacology; D000907:Antibodies, Bacterial / Q000276:immunology*; D001628:Beverages / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019453:Escherichia coli O157 / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification*; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D027845:Malus / Q000382:microbiology*; D012680:Sensitivity and Specificity
chemical_list: D000143:Acids; D000907:Antibodies, Bacterial