title: Neural stem cells suppress the hearing threshold shift caused by cochlear ischemia.
abstract: Neural stem cells are multipotent progenitor cells that show self-renewal activity. In this study, we assessed the use of neural stem cells for ameliorating ischemia-reperfusion injury of the gerbil cochlea. Neural stem cells were injected into one inner ear through the round window 1 day after ischemic insult. Immunostaining for nestin showed that the distribution of neural stem cells was concentrated within the organ of Corti. Seven days after ischemia, the injury-induced auditory brainstem response threshold shift and progressive inner hair cell damage were markedly less on the neural stem cell-transplanted side. These results suggest that the transplantation of neural stem cells is therapeutically useful for preventing damage to hair cells that occurs after transient ischemia of the cochlea.
authors.affiliation: Department of Otolaryngology, Division of Functional Histology, Ehime University School of Medicine, Toon, Ehime, Japan. hakubax@m.ehime-u.ac.jp
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hakuba
authors.forename: Ryuji
authors.initials: R
authors.lastname: Hata
authors.forename: Isamu
authors.initials: I
authors.lastname: Morizane
authors.forename: Gu
authors.initials: G
authors.lastname: Feng
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Kensuke
authors.initials: K
authors.lastname: Fujita
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakanaka
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Gyo
mesh_terms: D000818:Animals; D001309:Auditory Threshold / Q000502:physiology*; D003051:Cochlea / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery / Q000648:ultrastructure; D004195:Disease Models, Animal; D004427:Ear Diseases / Q000503:physiopathology*; D004622:Embryo, Mammalian; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology / Q000528:radiation effects; D005849:Gerbillinae; D006199:Hair Cells, Auditory, Inner / Q000378:metabolism / Q000503:physiopathology / Q000648:ultrastructure; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism; D007511:Ischemia / Q000601:surgery*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D064231:Nestin