Proposal of Yaniaceae fam. nov. and Yania flava sp. nov. and emended description of the genus Yania.
A coccoid actinobacterium strain (designated YIM 70178(T)) was isolated from a soil sample collected in Qinghai Province, China. The isolate grew well with an optimum salt concentration of 10-15% (KCl, w/v) but scarcely or not at all without salt. The cell-wall peptidoglycan type was A4alpha, L-Lys-gly-L-Glu. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unknown phospholipid and an unknown glycolipid. The predominant menaquinones were MK-8 and MK-9. The major fatty acid was anteiso-C(15:0). The DNA G+C content was 57.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 70178(T) was most closely related to the type strain of Yania halotolerans. DNA-DNA hybridization and comparison of physiological and chemotaxonomic characteristics demonstrated that strain YIM 70178(T) was different from Yania halotolerans. The name Yania flava sp. nov. is proposed, with strain YIM 70178(T) (=DSM 16377(T)=KCTC 19047(T)) as the type strain. Based on the phenotypic characteristics and phylogenetic position, as determined by 16S rRNA gene analysis and 16S rRNA signature nucleotide data, the genus description of Yania is therefore emended and strains YIM 70085(T) and YIM 70178(T) represent a novel family of the suborder Micrococcineae, for which the name Yaniaceae fam. nov. is proposed.
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Schumann
Peter
P
DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Zhang
Yu-Qin
YQ
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Xu
Ping
P
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Chen
Guo-Zhong
GZ
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Xu
Li-Hua
LH
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Stackebrandt
Erko
E
DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Jiang
Cheng-Lin
CL
The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S