Human herpesvirus 8 serological markers and viral load in patients with AIDS-associated Kaposi's sarcoma in Central African Republic.
Epidemic Kaposi's sarcoma (KS) is one of the most frequent types of cancer in several African countries; however, very few data are available on human herpesvirus 8 (HHV-8) markers in KS patients from Central Africa. In a series of 36 AIDS-KS cases from Central African Republic, we showed, using a real-time PCR quantitative assay, the high frequency (82%) of detectable HHV-8 DNA in peripheral blood mononuclear cells (PBMCs). We also found that the level of antibodies directed against lytic or latent HHV-8 antigens is not correlated to the amount of HHV-8 viral load in the PBMCs, and finally, we demonstrated a much higher viral load in tumoral skin lesions (6.07 log copies/mug DNA) than in unaffected skin (2.93 log copies/mug DNA) or in PBMCs (2.55 log copies/mug DNA).
Duprez
Renan
R
Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Département Ecosytèmes et Epidémiologie des Maladies Infectieuses. Batiment Lwoff., Institut Pasteur, Paris, France.
Kassa-Kelembho
Eric
E
Plancoulaine
Sabine
S
Brière
Josette
J
Fossi
Mireille
M
Kobangue
Léon
L
Minsart
Pierre
P
Huerre
Michel
M
Gessain
Antoine
A
D017088:AIDS-Related Opportunistic Infections / Q000276:immunology / Q000821:virology*; D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D002488:Central African Republic; D002648:Child; D004279:DNA, Viral / Q000032:analysis / Q000097:blood*; D005260:Female; D015658:HIV Infections; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000276:immunology / Q000821:virology*; D012867:Skin / Q000821:virology; D012878:Skin Neoplasms / Q000821:virology*; D019562:Viral Load
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral