Monomolecular multimodal fluorescence-radioisotope imaging agents.
Diagnosis of diseases by different imaging methods can provide complementary information about the functional status of diseased tissues or organs. To overcome the current difficulties in coregistering images from different imaging modalities with a high degree of accuracy, we prepared near-infrared (NIR) monomolecular multimodal imaging agents (MOMIAs) consisting of a heptamethine carbocyanine and 111In-DOTA chelate that served as antennae for optical and scintigraphic imaging, respectively. Their spectral properties clearly show that coordination of indium to MOMIA increased the fluorescence intensity of the compounds. The MOMIAs are exceptionally stable in biological media and serum up to 24 h at 37 degrees C. Biodistribution of the compounds in mice obtained by fluorescence photon and gamma-counts demonstrated a similar distribution trend of the molecular probe in different tissues, suggesting that the detected fluorescence and gamma-emissions emanated from the same source (MOMIA). At 24 h postinjection, the MOMIAs were excreted by the renal and hepatobiliary systems and the blood level of a representative MOMIA was very low, thereby reducing background noise caused by circulating molecular probes. These findings demonstrate the feasibility of preparing single molecules with the capacity to emit discernible and diagnostic fluorescent and gamma-radiations for optical and nuclear imaging of living organisms.
Zhang
Zongren
Z
Department of Radiology, Washington University, St. Louis, Missouri 63110, USA.
Liang
Kexian
K
Bloch
Sharon
S
Berezin
Mikhail
M
Achilefu
Samuel
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry* / Q000493:pharmacokinetics; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D011868:Radioisotopes / Q000737:chemistry*; D013057:Spectrum Analysis; D014018:Tissue Distribution
D005456:Fluorescent Dyes; D011868:Radioisotopes