title: Core-shell CdS/Cd(OH)2 quantum dots: synthesis and bioconjugation to target red cells antigens.
abstract: We report a new and efficient methodology of labelling red blood cells, in order to investigate the expression of anti-A antigen, employing luminescent semiconductor nanocrystals. Highly luminescent and stable core-shell cadmium sulphide/cadmium hydroxide [CdS/CdS(OH)2] colloidal particles were obtained in the nanometre size range. The surface of these particles was characterized by using a monoclonal anti-A antibody via a one-step glutaraldehyde cross-linking procedure, followed by conjugation of the particles to red cells of blood groups A+, and O+. Laser scanning confocal microscopy images indicated that after conjugation for 30 min, A+ and erythrocytes presented different patterns of dual bright emission whereas the O+ group cells showed no emission. We suggest that this labelling procedure may be applied as a quantitative tool to investigate the distribution and expression of alloantigen in red blood cells.
authors.affiliation: Departamento de Biofísica e Radiobiologia - CCB, Federal University of Pernambuco, Cid. Universitária CEP 50670-901 Recife-PE, Brazil.
authors.forename: P M Albuquerque
authors.initials: PM
authors.lastname: de Farias
authors.forename: B Saegesser
authors.initials: BS
authors.lastname: Santos
authors.forename: F Duarte
authors.initials: FD
authors.lastname: de Menezes
authors.forename: R de Carvalho
authors.initials: Rde C
authors.lastname: Ferreira
authors.forename: M de Lourdes
authors.initials: Mde L
authors.lastname: Barjas-Castro
authors.forename: V
authors.initials: V
authors.lastname: Castro
authors.forename: P R Moura
authors.initials: PR
authors.lastname: Lima
authors.forename: A
authors.initials: A
authors.lastname: Fontes
authors.forename: C L
authors.initials: CL
authors.lastname: Cesar
mesh_terms: D000497:Alloys; D000911:Antibodies, Monoclonal / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000032:analysis; D002104:Cadmium / Q000737:chemistry; D002863:Chromogenic Compounds / Q000138:chemical synthesis* / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D036103:Nanotechnology / Q000379:methods*; D045663:Quantum Dots
chemical_list: D000497:Alloys; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002863:Chromogenic Compounds; D002104:Cadmium