Enhancing immune responses against a plasmid DNA vaccine encoding a FMDV empty capsid from serotype O.
Various parameters have been examined for the improvement of immune responses induced by immunisation with foot-and-mouth disease virus (FMDV) DNA vaccine (pcDNA 3.1/P1-2A3C3D, P1) in swine. Our findings show that increasing the amount of P1 DNA plasmids and pGMCSF adjuvant plasmids induces stronger FMDV specific and neutralising antibody responses, as well as promoting cytokines IL-8 and IFNgamma secretion, in immunised pigs via multiple inoculation sites.
Li
Yanmin
Y
FMD Vaccine Group, Pirbright Laboratory, Institute for Animal Health, Ash Road, Woking, Surrey GU24 0NF, UK.
Aggarwal
Neeraj
N
Takamatsu
Haru-H
HH
Sterling
Catrina M A
CM
Voyce
Charlotte
C
Barnett
Paul V
PV
D000914:Antibodies, Viral / Q000097:blood; D002213:Capsid; D016207:Cytokines / Q000096:biosynthesis; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D009500:Neutralization Tests; D010957:Plasmids; D019444:Vaccines, DNA / Q000276:immunology*
D000914:Antibodies, Viral; D016207:Cytokines; D019444:Vaccines, DNA