title: Simultaneous determination of everolimus and cyclosporine concentrations by HPLC with ultraviolet detection.
abstract: In the clinical practice of organ transplantation everolimus (RAD) is used in combination with cyclosporine (CsA), the most common antirejection agent. Both drugs show a narrow therapeutic window, which requires strict monitoring of their blood concentration. Simple methods for simultaneous measurement of RAD and CsA concentration are needed. As we have recently developed an HPLC-UV assay for RAD determination, we decided to implement it to allow concomitant measurement of CsA. The within- and between-day coefficients of variation of the measurement were less than 12.1% for RAD and 9.8% for CsA. The within- and between-day inaccuracy of quality control samples were less than 9.7% for RAD and less than 4.9% for CsA. The method was found accurate and precise and useful for simultaneous therapeutic monitoring of the two drugs.
authors.affiliation: Department of Medicine and Transplantation, Ospedali Riuniti di Bergamo -, Mario Negri Institute for Pharmacological Research Via Gavazzeni, 11 - 24125, Bergamo, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Baldelli
authors.forename: Stefania
authors.initials: S
authors.lastname: Zenoni
authors.forename: Simona
authors.initials: S
authors.lastname: Merlini
authors.forename: Norberto
authors.initials: N
authors.lastname: Perico
authors.forename: Dario
authors.initials: D
authors.lastname: Cattaneo
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016572:Cyclosporine / Q000097:blood*; D000068338:Everolimus; D006801:Humans; D015203:Reproducibility of Results; D020123:Sirolimus / Q000031:analogs & derivatives* / Q000097:blood; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D016572:Cyclosporine; D000068338:Everolimus; D020123:Sirolimus