Overview of the spaceflight radiation environment and its impact on cell biology experiments.
Variables studied in typical cellular radiation biology experiments are cell killing, mutagenesis, transformation to malignancy, heritable damage, and DNA damage and repair. Dose response curves for cells exposed to low-LET radiations and some high LET radiations are well known. The low-LET dose rate in low earth orbit is roughly 1.0 mSv/day, the heavy-ion (Z>2) flux is about 1.0 particle/cm2-s corresponding to about 0.3 mSv/day, and the integrated neutron flux is roughly 2 neutrons/cm2-s corresponding to 0.012 mGy/d or, assuming a QF of 10, 0.12 mSv/d. Published dose-response curves were used to estimate the probability that a mammalian cell will be affected by each of the above types of damage. As a general approximation the exposure of an experimental cell population to the space radiation environment for 100 days will result in the following probabilities of damage per cell: cell killing based on clonogenicity 0.02, mutagenesis per locus based on phenotype analysis 1 x 10(-6), point mutation induction 2 x 10(-8) per locus, malignant transformation in vitro based on colony morphology 1.2 x 10(-5), heritable damage based on colony size 0.02, and induced DNA double-strand breaks based on fragment analysis by electrophoresis 3.5/cell or 0.26/cell after repair. Most of these figures are accurate to within a factor of 2. Thus the spaceflight radiation environment has essentially undetectable impact on typical cell biology experiments unless experimental goals involve the precise measurement of one of the above end-points. Other in vitro end-points, such as tissue morphogenesis and cell differentiation, are expected to be similarly unaffected by the spaceflight radiation environment.
Todd
Paul
P
SHOT, Inc., Greenville, IN 47124, USA. ptodd@shot.com
D000818:Animals; D001419:Bacteria / Q000166:cytology / Q000528:radiation effects; D016923:Cell Death / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D002468:Cell Physiological Phenomena / Q000528:radiation effects*; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects; D003359:Cosmic Radiation; D006224:Cricetinae; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D018499:Linear Energy Transfer; D016296:Mutagenesis; D009502:Neutrons; D011829:Radiation Dosage; D013026:Space Flight; D015003:Yeasts / Q000166:cytology / Q000528:radiation effects