title: Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast.
abstract: Two rounds of chromosome segregation after only a single round of DNA replication enable the production of haploid gametes from diploid precursors during meiosis. To identify genes involved in meiotic chromosome segregation, we developed an efficient strategy to knock out genes in the fission yeast on a large scale. We used this technique to delete 180 functionally uncharacterized genes whose expression is upregulated during meiosis. Deletion of two genes, sgo1 and mde2, caused massive chromosome missegregation. sgo1 is required for retention of centromeric sister-chromatid cohesion after anaphase I. We show here that mde2 is required for formation of the double-strand breaks necessary for meiotic recombination.
authors.affiliation: Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
authors.forename: Juraj
authors.initials: J
authors.lastname: Gregan
authors.forename: Peter K
authors.initials: PK
authors.lastname: Rabitsch
authors.forename: Benjamin
authors.initials: B
authors.lastname: Sakem
authors.forename: Ortansa
authors.initials: O
authors.lastname: Csutak
authors.forename: Vitaly
authors.initials: V
authors.lastname: Latypov
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Lehmann
authors.forename: Juerg
authors.initials: J
authors.lastname: Kohli
authors.forename: Kim
authors.initials: K
authors.lastname: Nasmyth
mesh_terms: D002842:Chromatids / Q000235:genetics / Q000502:physiology; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D020090:Chromosome Segregation / Q000235:genetics*; D017931:DNA Primers; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006921:Hygromycin B; D008540:Meiosis / Q000235:genetics*; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013309:Streptothricins
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers; D051858:Forkhead Transcription Factors; D029702:Schizosaccharomyces pombe Proteins; C485973:Sgo1 protein, S pombe; C509497:mde2 protein, S. pombe; D049452:Green Fluorescent Proteins; D006921:Hygromycin B; D013309:Streptothricins