title: Detection of nitrous oxide in the neuronal nitric oxide synthase reaction by gas chromatography-mass spectrometry.
abstract: Using headspace gas chromatography-mass spectrometry, we detected significant amounts of nitrous oxide in the reaction products of the monooxygenase reaction catalyzed by neuronal nitric oxide synthase. Nitrous oxide is a dimerization product of nitroxyl anion; its presence in the reaction products indicates that the nitroxyl anion is a product of the neuronal nitric oxide synthase-catalyzed reaction.
authors.affiliation: Department of Biochemistry, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA.
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Ishimura
authors.forename: Ying Tong
authors.initials: YT
authors.lastname: Gao
authors.forename: Satya P
authors.initials: SP
authors.lastname: Panda
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Roman
authors.forename: Bettie Sue S
authors.initials: BS
authors.lastname: Masters
authors.forename: Susan T
authors.initials: ST
authors.lastname: Weintraub
mesh_terms: D000818:Animals; D004926:Escherichia coli; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D009609:Nitrous Oxide / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D052248:Nitric Oxide Synthase Type I; D009609:Nitrous Oxide