Four viral proteins of white spot syndrome virus (WSSV) that attach to shrimp cell membranes.
White spot syndrome virus (WSSV) is a major shrimp pathogen that has a widespread negative affect on shrimp production in Asia and the Americas. It is known that WSSV infects shrimp cells through viral attachment proteins (VAP) that bind with shrimp cell receptors. However, the identity of both WSSV VAP and shrimp cell receptors remains unclear. We used digoxigenin (DIG)-labeled shrimp hemocyte and gill cell membranes to bind to WSSV proteins immobilized on nitrocellulose membranes, and 4 putative WSSV VAP (37 kDa, 39 kDa and 2 above 97 kDa) were identified. Mass spectrometric analysis identified the 37 kDa putative VAP as the product of WSSV gene VP281.
Liang
Yan
Y
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
Huang
Jie
J
Song
Xiao-Ling
XL
Zhang
Pei-Jun
PJ
Xu
Huai-Su
HS
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism*; D002681:China; D004076:Digoxigenin; D005880:Gills / Q000166:cytology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000821:virology*; D020539:Sequence Analysis, Protein; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000378:metabolism*
D014764:Viral Proteins; D004076:Digoxigenin