An improved arbitrary primed PCR method for rapid characterization of transposon insertion sites.
Modifications were made to published arbitrary primed polymerase chain reaction (AP-PCR) procedures that resulted in increased specificity and sensitivity. Several arbitrary primer sequences were also evaluated, resulting in recommendations for primer design.
Das
Sankar
S
Philips Institute of Oral and Craniofacial Molecular Biology, Virginia Commonwealth University, Richmond, VA 23298-0566, USA.
Noe
Jody C
JC
Paik
Sehmi
S
Kitten
Todd
T
D017931:DNA Primers; D004251:DNA Transposable Elements / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013298:Streptococcus sanguis / Q000235:genetics
D017931:DNA Primers; D004251:DNA Transposable Elements