Molecular characterization of Clostridium tetani strains by pulsed-field gel electrophoresis and colony PCR.
Pulsed-field gel electrophoresis and PCR were applied for the first time to the molecular characterization of Clostridium tetani. Among five strains tested, one (CN1339) turned out to contain a mixture of two genetically different clones and two (D11 and G761) to contain bacteria differing by the presence or absence of the 74-kb plasmid harboring the tetX gene.
Plourde-Owobi
Lucile
L
Research Department, Aventis Pasteur SA, av. Marcel Mérieux, Marcy L'Etoile 69280, France.
Seguin
Delphine
D
Baudin
Marie-Anne
MA
Moste
Catherine
C
Rokbi
Bachra
B
D015373:Bacterial Typing Techniques; D003017:Clostridium tetani / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D013744:Tetanus Toxin / Q000235:genetics*
D003470:Culture Media; D004269:DNA, Bacterial; D013744:Tetanus Toxin