Role of E-cadherin molecules in spheroid formation of hepatocytes adhered on galactose-carrying polymer as an artificial asialoglycoprotein model.
The role of E-cadherin in the spheroid formation of hepatocytes adhered on the poly(N-p-vinylbenzyl-D-lactonamide) (PVLA) as a model ligand for asialoglycoprotein receptors (ASGP-R) of hepatocytes was studied. Expression of E-cadherin was increased in round hepatocytes adhered on a high-coating density of PVLA (100 microg/ml), and also in flat ones adhered on a low-coating density of PVLA (1 microg/ml) in the presence of epidermal growth factor (EGF). Hepatocyte spheroids formed on the high-coating density of PVLA in the presence of EGF after 48 h were inhibited by an anti-E-cadherin monoclonal antibody (ECCD-1). From immunofluorescence and confocal laser microscopy, E-cadherin was localized in the intercellular boundaries and concentrated at the inside surface of aggregated cells. As a result, E-cadherin could play an important role in hepatocyte assembly.
Takei
R
R
Department of Biomolecular Engineering, Tokyo Institute of Technology, 226-8501, Yokohama, Japan.
Suzuki
D
D
Hoshiba
T
T
Nagaoka
M
M
Seo
S J
SJ
Cho
C S
CS
Akaike
T
T
D001212:Asialoglycoproteins / Q000737:chemistry / Q000378:metabolism; D032701:Biomimetics / Q000379:methods; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005690:Galactose / Q000737:chemistry / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism*; D007785:Lactose / Q000031:analogs & derivatives / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D018874:Spheroids, Cellular / Q000166:cytology* / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*
D001212:Asialoglycoproteins; D015820:Cadherins; D020099:Coated Materials, Biocompatible; D011137:Polystyrenes; C074112:poly-N-4-vinylbenzyllactonamide; D007785:Lactose; D005690:Galactose