Preparation of a novel PEG-clay hybrid as a DDS material: dispersion stability and sustained release profiles.
An advanced hybrid drug carrier has been developed using porous nanocrystals of a swelling clay mineral conjugated with a block copolymer containing poly(ethylene glycol) and polyamine segments. Synthetic hectorite (Laponite) modified with (alpha-acetal-poly(ethylene glycol)-block-[poly(2-(N,N-dimethylamino) ethyl methacrylate)] (Acetal-PEG-b-PAMA) produced a homogeneous dispersion of organic-inorganic hybrid in an aqueous solution, which showed flocculation-resistive properties with an elevated ionic strength. The zeta-potential measurement revealed that nonionic PEG brush layers are formed on the surface of the clay nanocrystals since negative charge of the clay surface was completely neutralized by the positive charge of the cationic PAMA segment and the entire surface charge is successfully shielded by the effect of nonionic PEG segment in the block copolymer. This charge neutralization is in good agreement with the dispersion stability in solutions of high ionic strength. The average particle size of the PEG-modified hybrid particle was estimated to be 120 nm by a dynamic light scattering (DLS) method. When pyrene was used as the model compound of hydrophobic drug, it was incorporated into the nanopore in the clay mineral without showing any remarkable expansion of the basal spacings. Fluorescence spectra and powder X-ray diffraction patterns demonstrated that pyrene molecules are captured in an amorphous state in the range of low pyrene content (<5%), while excimer formation was seen at the higher pyrene concentration (>5%). The PEG-clay hybrid act as a carrier for sustained release of hydrophobic substances due to the high affinity (K = 1.52 x 10(4)) between the drug and clay surface.
Takahashi
Tadahito
T
Pre-venture Program, Japan Science and Technology Corporation, JST, Japan.
Yamada
Yoshiaki
Y
Kataoka
Kazunori
K
Nagasaki
Yukio
Y
D000465:Algorithms; D000538:Aluminum Silicates / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D000077215:Clay; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D004355:Drug Stability; D004563:Electrochemistry; D005079:Excipients; D008968:Molecular Conformation; D009391:Nephelometry and Turbidimetry; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D011721:Pyrenes / Q000737:chemistry; D012995:Solubility; D012996:Solutions
D000538:Aluminum Silicates; D003692:Delayed-Action Preparations; D005079:Excipients; D011721:Pyrenes; D012996:Solutions; D011092:Polyethylene Glycols; D000077215:Clay