title: Use of enamel matrix protein derivative before dental reimplantation: a histometric analysis.
abstract: The use of enamel matrix protein in the treatment of periodontal defects has shown a favorable action on the proliferation of periodontal ligament cells, as well as on collagen formation and mineralization. The goal was to evaluate, histologically and histometrically, periodontal tissue regeneration after dental reimplantation using enamel matrix protein derivative (Emdogain, Biora AB, Malmö, Sweden). Male rats (Albinus, Wistar), weighing between 180 and 200 g, were divided in 3 groups. Animals in group I (control) had the upper right incisors extracted, the root canal was sealed with calcium hydroxide, and teeth were reimplanted in their alveoli. Group II underwent the same procedure, but the remaining periodontal ligament was removed from the root surfaces by root planing before reimplantation. In group III,following removal of the periodontal ligament, Emdogain was applied to the root surfaces. Animals were sacrificed 7, 20, and 60 days after reimplantation, and the alveoli were fixed, processed, and stained with hematoxylin and eosin. Formation of periodontal ligament, resorption areas, and ankylosis were analyzed. The results showed that group I (control) was better than groups II and III, with statistically significant differences on days 7 and 20 after reimplantation for formation of periodontal ligament. It may be concluded that with the methodology used, Emdogain was unable to stimulate tissue repair in reimplanted teeth.
authors.affiliation: University of São Paulo-School of Dentistry of Ribeirão Preto-USP, Av. do Café, s/n 14040-904 Ribeirão Preto, São Paulo, Brazil.
authors.forename: Gustavo Otoboni
authors.initials: GO
authors.lastname: Molina
authors.forename: Luiz Guilherme
authors.initials: LG
authors.lastname: Brentegani
mesh_terms: D000539:Alveolar Process / Q000187:drug effects / Q000503:physiopathology; D000818:Animals; D000844:Ankylosis / Q000473:pathology / Q000503:physiopathology; D018786:Bone Substitutes / Q000627:therapeutic use*; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D003746:Dental Enamel Proteins / Q000627:therapeutic use*; D007180:Incisor; D008297:Male; D010513:Periodontal Ligament / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects; D012390:Root Canal Therapy; D016745:Root Planing; D013997:Time Factors; D014090:Tooth Replantation; D014091:Tooth Resorption / Q000473:pathology / Q000503:physiopathology
chemical_list: D018786:Bone Substitutes; D003746:Dental Enamel Proteins; C109069:enamel matrix proteins