title: Genome-wide localization of histone 4 arginine 3 methylation in a differentiation primed myeloid leukemia cell line.
abstract: Methylation of arginine residues in proteins is involved in modulation of various protein-protein interactions. At the chromatin level H4R3 methylation provides a signal integration step during myeloid differentiation. In order to globally characterize the role of arginine methylation in signal integration and developmental processes we decided to map genomic loci marked by protein arginine methyl transferase 1 (PRMT1) via histone H4 arginine 3 methylation. For this, we used the myeloid leukemia cell line, HL60, which is known to differentiate along the monocyte/macrophage or granulocyte lineage. We used chromatin immunoprecipitation with an antibody specific for the H4 arginine 3 methyl epitope followed by cloning to isolate genomic loci marked by this modification. After sequencing and in silico analysis we found that all of the genomic hits identified were intronic or within 5 kb of 5' ends of specific genes. The locations identified were enriched in conserved transcription factor binding sites of POU2F1, MEF-2 and FOXL1 factors. A significant number of the genes in the proximity of the identified genomic loci are involved in signaling pathways and developmental processes including immune response of myeloid cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, University of Debrecen, Medical and Health Science Center, Nagyerdei krt. 98. Debrecen, H-4012 Hungary.
authors.forename: Balint L
authors.initials: BL
authors.lastname: Balint
authors.forename: Petra
authors.initials: P
authors.lastname: Gabor
authors.forename: Laszlo
authors.initials: L
authors.lastname: Nagy
mesh_terms: D001120:Arginine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D002874:Chromosome Mapping; D019175:DNA Methylation; D005786:Gene Expression Regulation; D018922:HL-60 Cells; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D008969:Molecular Sequence Data; D011484:Protein-Arginine N-Methyltransferases / Q000378:metabolism
chemical_list: D006657:Histones; D001120:Arginine; D011484:Protein-Arginine N-Methyltransferases