title: [Localization and pathological examination of sentinel lymph node during operation in gastric cancer].
abstract: OBJECTIVE
  To evaluate the application of sentinel lymph node (SLN) biopsy in diagnosis and treatment of gastric cancer.
  
  
  METHODS
  Thirty- eight patients with gastric cancer were divided into three groups (T(1); T(2); T(3)) according to tumor invasion depth. SLN was localized using methyl blue injection method. Metastatic lymph nodes were detected by immunohistochemical staining of cytokeratin (CK- 19).
  
  
  RESULTS
  SLN(S ) were detected in all 38 patients and lymph node metastasis occurred in 18 cases. The sensitivity, false- negative rate, and diagnostic accuracy of SLN for lymph node metastasis were 83.3% 16.7% and 92.1% respectively. The diagnostic accuracy was 100% in T1 cases without false- negative cases, 94.1% in T(2) cases with one false- negative case, 75% in T(3) cases with two false- negative cases.
  
  
  CONCLUSIONS
  The method of injecting methylene blue around the primary tumor is a feasible method that can determine SLN localization during operation.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Dalian Medical University, Dalian 110611, China. fuxibo@sina.com.cn
authors.forename: Xi-bo
authors.initials: XB
authors.lastname: Fu
authors.forename: Xiang
authors.initials: X
authors.lastname: Hu
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008751:Methylene Blue; D008875:Middle Aged; D009367:Neoplasm Staging; D021701:Sentinel Lymph Node Biopsy; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000473:pathology*
chemical_list: D008751:Methylene Blue