Experimental studies on artificial blood usage for hemodilution during cardiopulmonary bypass.
BACKGROUND
Although hemodilution is usually utilized during cardiopulmonary bypass (CPB), hemodilution can cause adverse effects such as hypotension and hypoxia. The purpose of this study was to evaluate a novel perfluoro-octyl bromide (PFOB) emulsion, one of perfluorochemicals (PFCs) emulsions, administered during hemodilution CPB.


METHODS
Fifteen dogs were subjected to CPB for 2 hours under mild hypothermia. Animals were divided into three groups; control group, hemodilution group and PFOB group. During the experiment, hemodynamics, complete blood count and blood chemistry were monitored. In addition, serum complement titer (CH50), bradykinin and histamine concentrations were also measured.


RESULTS
Heart rate (HR) was markedly elevated in the hemodilution groups (p<0.05). Mean arterial pressure (MAP) did not change in the three groups. White blood cell (WBC) and platelet (PLT) count did not significantly differ among the three groups. Plasma lactate concentration was markedly elevated only in hemodilution group during late phase of CPB (p<0.05). In the hemodilution group, CH50, bradykinin and histamine, were markedly elevated during the CPB and just after CPB (p<0.05).


CONCLUSIONS
The present study demonstrated possible benefits of the new PFC emulsion during cardiac surgery by counteracting the adverse effects of hemodilution during CPB.
Isaka
Mitsuhiro
M
Department of Cardiovascular Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
Sakuma
Ichiro
I
Imamura
Michiaki
M
Makino
Yutaka
Y
Fukushima
Shoji
S
Nakai
Kunihiko
K
Shiiya
Norihiko
N
Kenmotsu
Osamu
O
Kitabatake
Akira
A
Yasuda
Keishu
K
D000704:Analysis of Variance; D000818:Animals; D001802:Blood Substitutes / Q000008:administration & dosage*; D001920:Bradykinin / Q000032:analysis / Q000378:metabolism; D002315:Cardiopulmonary Bypass / Q000379:methods*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D006438:Hemodilution / Q000379:methods*; D006439:Hemodynamics / Q000502:physiology*; D006632:Histamine / Q000032:analysis / Q000378:metabolism; D011336:Probability; D011897:Random Allocation; D012016:Reference Values; D012307:Risk Factors; D012680:Sensitivity and Specificity
D001802:Blood Substitutes; D006632:Histamine; D001920:Bradykinin