The Arabidopsis R2R3 MYB proteins FOUR LIPS and MYB88 restrict divisions late in the stomatal cell lineage.
The two guard cells of a stoma are produced by a single symmetric division just before terminal differentiation. Recessive mutations in the FOUR LIPS (FLP) gene abnormally induce at least four guard cells in contact with one another. These pattern defects result from a persistence of precursor cell identity that leads to extra symmetric divisions at the end of the cell lineage. FLP is likely to be required for the correct timing of the transition from cell cycling to terminal differentiation. FLP encodes a two-repeat (R2R3) MYB protein whose expression accumulates just before the symmetric division. A paralogous gene, MYB88, overlaps with FLP function in generating normal stomatal patterning. Plants homozygous for mutations in both genes exhibit more severe defects than flp alone, and transformation of flp plants with a genomic MYB88 construct restores a wild-type phenotype. Both genes compose a distinct and relatively basal clade of atypical R2R3 MYB proteins that possess an unusual pattern of amino acid substitutions in their putative DNA binding domains. Our results suggest that two related transcription factors jointly restrict divisions late in the Arabidopsis thaliana stomatal cell lineage.
Lai
Lien B
LB
Department of Plant Cellular and Molecular Biology, Ohio State University, Columbus, OH 43210, USA.
Nadeau
Jeanette A
JA
Lucas
Jessica
J
Lee
Eun-Kyoung
EK
Nakagawa
Tsuyoshi
T
Zhao
Liming
L
Geisler
Matt
M
Sack
Fred D
FD
D019943:Amino Acid Substitution / Q000235:genetics; D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D017124:Conserved Sequence; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D010641:Phenotype; D019441:Plant Epidermis / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D018515:Plant Leaves / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014170:Transformation, Genetic / Q000235:genetics
C511988:ATR1 protein, Arabidopsis; D029681:Arabidopsis Proteins; C511989:CPC protein, Arabidopsis; D018797:Cell Cycle Proteins; C507998:FLP protein, Arabidopsis; C507999:Myb88 protein, Arabidopsis; D010940:Plant Proteins; D020598:Proto-Oncogene Proteins c-myb; D014157:Transcription Factors