title: Numerically simulated pH-induced reactivation of catalytic activity of horseradish peroxidase.
abstract: A model mechanism that accounts for the experimentally observed pH-dependent enhancement of enzymatic activity of horseradish peroxidase through the catalase-like pathway is proposed. Predictions of the model are tested against a number of experimental results to confirm that kinetic constants used in the numerical simulation are correctly chosen and that the model can be used to emulate the reaction between horseradish peroxidase and hydrogen peroxide in a wide range of conditions.
authors.affiliation: Faculty of Physical Chemistry, University of Belgrade, Serbia and Montenegro. vladana.vukojevic@cmm.ki.se
authors.forename: Ana
authors.initials: A
authors.lastname: Popovic-Bijelić
authors.forename: Goran
authors.initials: G
authors.lastname: Bijelić
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Kolar-Anić
authors.forename: Vladana
authors.initials: V
authors.lastname: Vukojević
mesh_terms: D002384:Catalysis; D003198:Computer Simulation; D004789:Enzyme Activation / Q000187:drug effects*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D013053:Spectrophotometry
chemical_list: D016877:Oxidants; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase