title: Mitsuaria chitosanitabida gen. nov., sp. nov., an aerobic, chitosanase-producing member of the 'Betaproteobacteria'.
abstract: Four strains (3001(T), 2, 12 and 13), which were isolated as chitosanase-producing bacteria from soil from Matsue city (Japan), were studied phenotypically, genotypically and phylogenetically. Based on sequence analysis of 16S rRNA genes, DNA G+C content (67.4-69.2 mol%), quinone type (UQ-8), major fatty acid composition (3-OH 10:0, 3-OH 14:0) and other phylogenetic studies, strains 3001(T), 12 and 13 were found to occupy a separate position in the 'Betaproteobacteria'. Roseateles depolymerans, Rubrivivax gelatinosus and Ideonella dechloratans were their closest neighbours (93-95% 16S rRNA gene sequence similarity). The 16S rRNA gene sequence and other characteristics suggested that strain 2 belonged to the genus Flavobacterium. DNA-DNA hybridization experiments supported the conclusion that strains 3001(T), 12 and 13 were of the same species (72-78% DNA hybridization) and only distantly related to I. dechloratans and R. gelatinosus. It is proposed that strains 3001(T), 12 and 13 represent a novel genus and species for which the name Mitsuaria chitosanitabida gen. nov., sp. nov. is proposed. The type strain of Mitsuaria chitosanitabida is 3001(T) (=IAM 14711(T)=ATCC BAA-476(T)).
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
authors.forename: Daiki
authors.initials: D
authors.lastname: Amakata
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Matsuo
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Shimono
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
authors.forename: Jae Kweon
authors.initials: JK
authors.lastname: Park
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
authors.forename: Choong Soo
authors.initials: CS
authors.lastname: Yun
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsuda
authors.affiliation: Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Yokota
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawamukai
mesh_terms: D001420:Bacteria, Aerobic / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D015373:Bacterial Typing Techniques; D020562:Betaproteobacteria / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D006026:Glycoside Hydrolases; C019868:chitosanase