title: Functional studies on a ketoreductase gene from Streptomyces sp. AM-7161 to control the stereochemistry in medermycin biosynthesis.
abstract: Medermycin shows the same trans (3S,15R) configuration as actinorhodin in the pyran ring crucial for its bioactivity. One medermycin biosynthetic gene, med-ORF12, is assumed to be involved in the stereochemical control at C-3. Functional complementation suggested that it plays a similar role as actVI-ORF1 previously proved to determine the stereospecificity at C-3 in actinorhodin biosynthesis. Co-expression of med-ORF12 with actinorhodin early biosynthetic genes further demonstrated that med-ORF12 encodes a ketoreductase responsible for the enantioselective reduction at C-3 in the formation of the pyran ring.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Tokyo 113-0033, Japan.
authors.forename: Aiying
authors.initials: A
authors.lastname: Li
authors.forename: Takayuki
authors.initials: T
authors.lastname: Itoh
authors.forename: Takaaki
authors.initials: T
authors.lastname: Taguchi
authors.forename: Ting
authors.initials: T
authors.lastname: Xiang
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ebizuka
authors.forename: Koji
authors.initials: K
authors.lastname: Ichinose
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D015394:Molecular Structure; D005810:Multigene Family; D009285:Naphthoquinones / Q000737:chemistry / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D013237:Stereoisomerism; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D000095622:Benzoisochromanequinones
chemical_list: D000429:Alcohol Oxidoreductases; D009285:Naphthoquinones; C013104:medermycin; D000095622:Benzoisochromanequinones