Uptake of cell-penetrating peptides in yeasts.
The uptake of different cell-penetrating peptides (CPPs) in two yeast species, Saccharomyces cerevisiae and Candida albicans, was studied using fluorescence HPLC-analyses of cell content. Comparison of the ability of penetratin, pVEC and (KFF)(3)K to traverse the yeast cell envelope shows that the cellular uptake of the peptides varies widely. Moreover, the intracellular degradation of the CPPs studied varies from complete stability to complete degradation. We show that intracellular degradation into membrane impermeable products can significantly contribute to the fluorescence signal. pVEC displayed highest internalizing capacity, and considering its stability in both yeast species, it is an attractive candidate for further studies.
Holm
Tina
T
Department of Neurochemistry and Neurotoxicology, Arrhenius Laboratories, Stockholm University, S-10691 Stockholm, Sweden.
Netzereab
Semharai
S
Hansen
Mats
M
Langel
Ulo
U
Hällbrink
Mattias
M
D000595:Amino Acid Sequence; D000818:Animals; D002176:Candida albicans / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002463:Cell Membrane Permeability; D057846:Cell-Penetrating Peptides; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013696:Temperature
D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides; D010446:Peptide Fragments; D010455:Peptides; C437783:pVEC peptide; C414409:penetratin