Rapid identification of Staphylococcus aureus in blood cultures by a combination of fluorescence in situ hybridization using peptide nucleic acid probes and flow cytometry.
Fluorescence in situ hybridization (FISH) using peptide nucleic acid probes (PNAs) allows the identification of Staphylococcus aureus from human blood culture samples. We present data revealing that the combination of PNA FISH and flow cytometry is a possible approach for the noncultural identification of staphylococci in blood cultures.
Hartmann
Hanna
H
Institut für Medizinische Mikrobiologie und Hygiene, Tübingen, Germany.
Stender
Henrik
H
Schäfer
Andrea
A
Autenrieth
Ingo B
IB
Kempf
Volkhard A J
VA
D016470:Bacteremia / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids / Q000235:genetics*; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013997:Time Factors
D003470:Culture Media; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids