title: Activation processing of cathepsin H impairs recognition by its propeptide.
abstract: Free propeptides are known to function as inhibitors of the parental mature cysteine cathepsins. This general rule, however, does not apply to the aminopeptidase cathepsin H. Screening of propeptide fragments for their inhibitory potency revealed no significant effect on the native mature cathepsin H. On the other hand, inhibitory interaction was established with recombinant cathepsin H that displays endopeptidase activity due to a lack of the mini-chain. This finding suggests that the propeptide-binding region is structurally rearranged during maturation processing and mini-chain formation, which impairs the effective recognition of mature cathepsin H by its own propeptide.
authors.affiliation: Department of Protein Biochemistry, Institute of Organic Chemistry and Biochemistry, Flemingovo nam. 2, CZ-16610 Praha, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Horn
authors.forename: Lucie
authors.initials: L
authors.lastname: Dolecková-Maresová
authors.forename: Lubomír
authors.initials: L
authors.lastname: Rulísek
authors.forename: Martin
authors.initials: M
authors.lastname: Mása
authors.forename: Olga
authors.initials: O
authors.lastname: Vasiljeva
authors.forename: Boris
authors.initials: B
authors.lastname: Turk
authors.forename: Tudeviin
authors.initials: T
authors.lastname: Gan-Erdene
authors.forename: Miroslav
authors.initials: M
authors.lastname: Baudys
authors.forename: Michael
authors.initials: M
authors.lastname: Mares
mesh_terms: D000595:Amino Acid Sequence; D056655:Cathepsin H; D002403:Cathepsins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D004792:Enzyme Precursors; D010455:Peptides; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D056655:Cathepsin H