Effect of different nuclear localization sequences on the immune responses induced by a MIDGE vector encoding bovine herpesvirus-1 glycoprotein D.
To optimize the efficiency of a Minimalistic Immunogenically Defined Gene Expression (MIDGE) vector, peptides containing proven (SV40 T-antigen and bovine herpesvirus-1 VP8) or putative (herpes simplex virus-1 VP22) nuclear localization signals (NLSs) were linked to a MIDGE vector encoding a truncated, secreted form of BHV-1 glycoprotein D (tgD) (MIDGE-tgD). Conjugation of an NLS to the MIDGE-tgD vector improved the tgD expression in vitro and the humoral and cellular immune responses induced in mice in vivo. The NLS from BHV-1 VP8 was most efficient at enhancing the tgD production and tgD-specific immune responses when conjugated to the MIDGE-tgD vector.
Zheng
Chunfu
C
Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Rd, Saskatoon, SK, Canada S7N 5E3.
Juhls
Christiane
C
Oswald
Detlef
D
Sack
Florian
F
Westfehling
Ines
I
Wittig
Burghardt
B
Babiuk
Lorne A
LA
van Drunen Littel-van den Hurk
Sylvia
S
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D005822:Genetic Vectors; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007111:Immunity, Cellular; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D019913:Nuclear Localization Signals; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins