[The influence of circadian gene mPeriod2 on Lewis cancer cell].
OBJECTIVE
To investigate the role of circadian gene mPeriod2 (mPer2) on tumor proliferation and apoptosis.


METHODS
The eukaryotic mPer2 expression vector (pcDNA 3.1-mPer2) based on pcDNA 3.1 was transfected into the cultured Lewis tumor cell by liposome method in vivo. The expression of mPer2 in transfected Lewis tumor cell was detected by immunohistochemistry and flowcytometry. The stable transfected cell line was screened by G418 and its proliferation and apoptosis was examined by flowcytometry.


RESULTS
Expression of PERIOD2 protein in pcDNA 3.1-mPer2 transfected Lewis tumor cell was proved by immunohistochemistry and flowcytometry. Flowcytometry result of mPer2 expression Lewis tumor cell showed less proliferation and high rate of apoptosis.


CONCLUSIONS
mPer2 expression can suppress the proliferation of tumor cell and increase the apoptosis of it.
Wang
Yue-qi
YQ
Hua
Hui
H
Zhu
Bin
B
Yin
Hua-hu
HH
Liu
Yan-you
YY
Yang
Chun-lei
CL
Wang
Zheng-rong
ZR
D000818:Animals; D017209:Apoptosis; D018827:Carcinoma, Lewis Lung / Q000235:genetics; D018797:Cell Cycle Proteins; D002455:Cell Division; D002940:Circadian Rhythm / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D056950:Period Circadian Proteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000276:immunology
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D009687:Nuclear Proteins; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D014157:Transcription Factors