title: Challenges in the testing of non-heart-beating cadavers for viral markers: implications for the safety of tissue donors.
abstract: Natural changes that occur in blood and tissue after death may result in false positive results in antigen and antibody detection tests performed to identify markers of viral infection in potential tissue donors. Such tissue, which might otherwise be acceptable for therapeutic purposes, would not meet current standards for safe tissue banking. This is especially important in the context of insufficiency in the tissue supply. In this study, a series of blood samples collected during routine post-mortem examination was assayed using a range of commercially available kits for the detection of HBsAg, anti-HCV and anti-HIV 1 + 2 antibody/antigen. Results of tests on 104 samples collected from 97 individuals indicate that some kits result in a higher number of initial reactive samples than others. Approximately 40% of samples were reactive in one or more HBsAg assay, less than 10% in at least one anti-HIV kit and only 1 sample at low level on an anti-HCV kit. Liver or lymph node samples from individuals whose serum sample gave reactive results in antigen/antibody assays were tested for viral nucleic acid in the corresponding nucleic acid amplification test. Only one individual's sample was confirmed to test positive for HBsAg in a confirmatory neutralisation test and by nucleic acid amplification technology, and a second individual whose serum was scored reactive for anti-HCV, but negative for HBsAg, had a liver sample which was HBV DNA positive and HCV RNA negative. The results of the study indicate that antibody/antigen assays are not as specific as NAT using state of the art DNA extraction techniques. Both types of assay complement each other and used together will help assure the safety of tissues for transplantation.
authors.affiliation: Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Herts EN6 3QG, UK.
authors.forename: David
authors.initials: D
authors.lastname: Padley
authors.forename: Morag
authors.initials: M
authors.lastname: Ferguson
authors.forename: Ruth M
authors.initials: RM
authors.lastname: Warwick
authors.forename: Christopher
authors.initials: C
authors.lastname: Womack
authors.forename: Sebastian B
authors.initials: SB
authors.lastname: Lucas
authors.forename: John
authors.initials: J
authors.lastname: Saldanha
mesh_terms: D015415:Biomarkers / Q000097:blood; D002102:Cadaver; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D015483:HIV Antibodies / Q000097:blood*; D015488:HIV Antigens / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D008099:Liver / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D021141:Nucleic Acid Amplification Techniques; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012449:Safety; D012698:Serologic Tests; D014019:Tissue Donors
chemical_list: D015415:Biomarkers; D004279:DNA, Viral; D015483:HIV Antibodies; D015488:HIV Antigens; D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic