title: Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism.
abstract: The enantioselectivity of a Burkholderia cepacia lipase toward secondary alcohols could be both increased and decreased rationally by introducing only a single mutation on the basis of the mechanism proposed previously.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Okayama University, Tsushima, Okayama 700-8530, Japan. ema@cc.okayama-u.ac.jp
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ema
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Fujii
authors.forename: Misa
authors.initials: M
authors.lastname: Ozaki
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Korenaga
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000438:Alcohols / Q000737:chemistry; D016956:Burkholderia cepacia / Q000201:enzymology; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008049:Lipase / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013237:Stereoisomerism
chemical_list: D000438:Alcohols; D004269:DNA, Bacterial; D008049:Lipase