Pellet-freezing of Damascus goat semen in a chemically defined extender.
During the breeding season of goats (12 bucks and 64 does) in Egypt, five experiments were conducted using a chemically defined cryoextender (CDE) to investigate: (1) the influence of rates of semen dilution (1:2, 1:4 and 1:19) and methods of thawing of frozen semen pellets (dry thawing versus wet thawing) on sperm progressive motility (SPM), sperm acrosome abnormalities (SAA) and rate of lipid peroxidation in semen as measured by malonaldehyde (MAL) production, and (2) the effect of insemination of does in natural (n = 38) and cloprostenol-synchronized (n = 26) estrus with frozen semen on their kidding rates and prolificacy. Semen (two successive ejaculates/buck) was collected twice a week via an AV and only ejaculates of >2500 x 10(6) sperm/ml and 70% SPM were diluted in one step at 30 degrees C with the CDE, cooled to 5 degrees C over a 4h-period, frozen in the form of 0.30 ml pellets and stored in liquid nitrogen for 72 h. The results revealed that post-thaw SPM of semen diluted at a rate of 1:4 was significantly (P < 0.01) higher than that of semen diluted at the other rates. Dilution of semen at a rate of 1:19 (< or =151 x 10(6) sperm/ml) not only minimized (P < 0.01) pre-freeze and post-thaw SPM, but also augmented (P < 0.01) pre-freeze and post-thaw rates of lipid peroxidation as evidenced by the high level of MAL production and the ability of antioxidants (1mg/ml EDTA, 200 U/ml bovine liver catalase, 0.61 mg/ml reduced glutathione and 0.11 mg/ml sodium pyruvate) to restore (P < 0.01) pre-freeze and post-thaw SPM. Frozen semen pellets exposed to dry thawing had a greater percentage of SPM (P < 0.01) as well as lower values of SAA and MAL (P < 0.01) than those exposed to wet thawing. Although the kidding rates did not vary significantly among does in natural (55.26%) and synchronized (53.85%) estrus, a higher (P < 0.05) prolificacy was obtained after their insemination in natural (1.81+/-0.16) rather than in synchronized (1.22+/-0.11) estrus.
Khalifa
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TA
Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. drtarekkhalifa@in.gr
El-Saidy
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BE
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D002084:Butylated Hydroxytoluene; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D004973:Estrus Synchronization; D005260:Female; D006041:Goats; D006358:Hot Temperature; D007315:Insemination, Artificial / Q000662:veterinary; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009584:Nitrogen; D011247:Pregnancy; D012661:Semen / Q000737:chemistry / Q000378:metabolism; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012996:Solutions; D013076:Sperm Count; D013081:Sperm Motility; D014325:Tromethamine
D000975:Antioxidants; D012996:Solutions; D014325:Tromethamine; D002084:Butylated Hydroxytoluene; D008315:Malondialdehyde; D009584:Nitrogen