Induction of multiple shoots from leaf segments, in vitro-flowering and fruiting of a dwarf tomato (Lycopersicon esculentum).
Multiple shoots were induced from leaf explants of Lycopersicon esculentum cultivar MicroTom, within 20-25d, on MS medium supplemented with 8.9 microM benzylaminopurine (BAP)+1.14 microM indole-3-acetic acid (IAA). For rooting, elongated microshoots were excised and transferred onto MS medium supplemented with 4.9 microM indole-3-butyric acid (IBA). Well-developed roots and flower raceme were obtained on d 7 and 13, respectively, upon transfer of the microshoots onto rooting medium. The flowers self-fertilized in vitro and produced mature fruits in additional 15-17d of culture.
Rao
Kokkirala Venugopal
KV
Department of Botany, Plant Biotechnology Research Unit, Kakatiya University, Warangal 506 009, India.
Kiranmayee
Kasula
K
Pavan
Umate
U
Sree
Telakalapalli Jaya
TJ
Rao
Alleni V
AV
Sadanandam
Abbagani
A
D035264:Flowers / Q000502:physiology*; D005638:Fruit / Q000502:physiology*; D007211:Indoles / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology*; D018520:Plant Shoots / Q000254:growth & development*; D012038:Regeneration
D007211:Indoles; C014612:indolebutyric acid