title: High prevalence of high-risk oncogenic human papillomaviruses harboring atypical distribution in women of childbearing age living in Libreville, Gabon.
abstract: The extent of human papillomavirus (HPV) genital shedding and type-specific diversity were evaluated in 354 consecutive women of childbearing age living in Libreville, Gabon. Detection of HPV DNA was performed by PCR using the MY09/MY11 primer set on DNA extracted from endocervical swabs. All PCR positive specimens were subjected to direct sequencing and HPV genotypes were identified on the basis of >95% sequence homology in the L1 region. Reverse line blot hybridization assay was used when a genotype could not be resolved by sequencing alone. HPV DNA was detected in 163 (46%) women, all clinically asymptomatic for HPV-related lesions. The highest prevalence of genital HPV detection (45%) was in the age group from 22 to 29 years. A total of 90 women (55%) harbored high-risk (HR) genotypes, with the most common being HPV-53 (19; 12%), HPV-58 (17; 11%), and HPV-16 (16; 10%). Low-risk genotypes were found in 36 (22%) women with HPV-54 and HPV-70 being the most frequently detected (17; 11% and 10; 6%, respectively). Finally 37 women (23%) tested positive for genotypes of unknown oncogenic risk, the most common in this category being HPV-83 (20; 12%). Multiple infections were detected in 35 (21%) women. By multivariate analysis, HPV genital shedding was significantly associated with young age (OR: 0.34; P < 0.007). The multivalent vaccine currently available against cervical carcinomas, is only active against HPV-16 and HPV-18, and will thus have a low impact in this setting.
authors.affiliation: Laboratoire de Virologie, Hôpital Européen Georges Pompidou, Paris, France. ali.simohamed@egp.ap-hop-paris.fr
authors.forename: Ali
authors.initials: A
authors.lastname: Si-Mohamed
authors.forename: Angélique
authors.initials: A
authors.lastname: Ndjoyi-Mbiguino
authors.forename: Kate
authors.initials: K
authors.lastname: Cuschieri
authors.forename: Isabelle Ndombi
authors.initials: IN
authors.lastname: Onas
authors.forename: Isabelle
authors.initials: I
authors.lastname: Colombet
authors.forename: Francis
authors.initials: F
authors.lastname: Ozouaki
authors.forename: Jérôme Le
authors.initials: JL
authors.lastname: Goff
authors.forename: Heather
authors.initials: H
authors.lastname: Cubie
authors.forename: Laurent
authors.initials: L
authors.lastname: Bélec
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D002584:Cervix Uteri / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005681:Gabon / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D015995:Prevalence; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology; D014621:Vagina / Q000821:virology; D014765:Viral Vaccines; D017201:Virus Shedding
chemical_list: D004279:DNA, Viral; D014765:Viral Vaccines