title: Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay.
abstract: A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for M. tuberculosis makes it more suitable method for MIC determination.
authors.affiliation: Biotechnology Division, Regional Research Laboratory, Jammu-Tawi 180001, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.forename: Inshad Ali
authors.initials: IA
authors.lastname: Khan
authors.forename: Vijashoor
authors.initials: V
authors.lastname: Verma
authors.forename: Narender
authors.initials: N
authors.lastname: Kalyan
authors.forename: Giulam Nabi
authors.initials: GN
authors.lastname: Qazi
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001681:Biological Assay / Q000379:methods; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D050901:Nitrate Reductase / Q000032:analysis* / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D014376:Tuberculosis / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D009566:Nitrates; D011933:Reagent Kits, Diagnostic; D050901:Nitrate Reductase