The mitosis and immunocytochemistry of olfactory ensheathing cells from nasal olfactory mucosa.
OBJECTIVE
To culture olfactory ensheathing cells (OECs) of rats in vitro and to investigate its morphology, mitosis and immunocytochemistry, and to explore if the OECs could be a new donation for transplantation.


METHODS
OECs were harvested from olfactory mucosa of Sprague Dawley rats based on the differing rates of attachment of the various cell types, followed by glial fibrillary acidic protein (GFAP), nerve growth factor (NGF), anti-low affinity receptor for NGF (NGFRp75), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and S-100 immunocytochemistry. The morphological changes and mitosis were observed under a phase contrast microscope at different culture time.


RESULTS
Three morphologically distinct types of cells, bipolar, multipolar and flat morphology were present in the primary culture of adult rat olfactory mucosa. Mitosis was characterized by a retraction of all processes, forming a sphere that divided into spherical daughter cells, the daughter cells sent out their processes. The OECs were immunoreactive for GFAP, NGFRp75, S-100, NGF, BDNF and NT-3.


CONCLUSIONS
The OECs from nasal olfactory mucosa cultivated in the medium with fetal bovine serum could survive, divide, differentiate, and express the neurotrophin. It may become an accessible source for autologous grafting in spinal cord injury.
Liu
Jin-bo
JB
Department of Orthopedics, Changzhou No. 2 Affiliated Hospital, Nanjing Medical University, Changzhou 213003, China. ZjLjB@163.com
Tang
Tian-si
TS
Gong
Ai-hua
AH
Sheng
Wei-hua
WH
Yang
Ji-cheng
JC
D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008297:Male; D008938:Mitosis; D009831:Olfactory Mucosa / Q000166:cytology* / Q000637:transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley