title: Repolarization gradients in the canine left ventricle before and after induction of short-term cardiac memory.
abstract: BACKGROUND
  Questions remain about the contributions of transmural versus apicobasal repolarization gradients to the configuration of the T wave in control settings and after the induction of short-term cardiac memory.
  
  
  RESULTS
  Short-term cardiac memory is seen as T-wave changes induced by altered ventricular activation that persists after restoration of sinus rhythm. We studied cardiac memory in anesthetized, open-chest dogs paced from the ventricle for 2 hours. Unipolar electrograms were recorded from as many as 98 epicardial and 144 intramural sites, and activation times and activation-recovery intervals (ARIs) were measured. In separate experiments, epicardial monophasic action potentials were recorded. We found no appreciable left ventricular intramural gradients in repolarization times (activation time+ARI) in either control conditions or after the induction of memory. In controls, there was a left ventricular apicobasal gradient, with the shortest repolarization times in anterobasal regions and longest repolarization times posteroapically. After induction of memory, repolarization times shortened uniformly throughout the ventricular wall. Monophasic action potential duration at 90% repolarization decreased by approximately 10 ms after induction of memory.
  
  
  CONCLUSIONS
  In the intact canine left ventricle at physiological rates, there is no transmural gradient in repolarization. Apicobasal gradients in repolarization time, with shortest repolarization times in anterobasal areas and longest repolarization times in posteroapical regions, are important in the genesis of the T wave. Repolarization times and monophasic action potentials at the 90% repolarization level shorten after the induction of memory. The deeper T wave in the ECG after induction of memory may be explained by the more rapid phase 3 of the action potential.
authors.affiliation: Center for Molecular Therapeutics, Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York, USA. m.j.janse@amc.uva.nl
authors.forename: Michiel J
authors.initials: MJ
authors.lastname: Janse
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Sosunov
authors.forename: Ruben
authors.initials: R
authors.lastname: Coronel
authors.forename: Tobias
authors.initials: T
authors.lastname: Opthof
authors.forename: Evgeny P
authors.initials: EP
authors.lastname: Anyukhovsky
authors.forename: Jacques M T
authors.initials: JM
authors.lastname: de Bakker
authors.forename: Alexei N
authors.initials: AN
authors.lastname: Plotnikov
authors.forename: Iryna N
authors.initials: IN
authors.lastname: Shlapakova
authors.forename: Peter
authors.initials: P
authors.lastname: Danilo
authors.forename: Jan G P
authors.initials: JG
authors.lastname: Tijssen
authors.forename: Michael R
authors.initials: MR
authors.lastname: Rosen
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001794:Blood Pressure; D004285:Dogs; D004562:Electrocardiography; D004597:Electroshock; D006329:Heart Conduction System / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D023421:Models, Animal; D016277:Ventricular Function, Left / Q000502:physiology*