Live lambs born from zona-pellucida denuded embryos.
Progress with techniques using zona-pellucida denuded embryos has resulted in the birth of live cattle, pigs, and mice. The application of zona-free methods in sheep has been restricted to in vitro studies. In this report, we demonstrate that live lambs can be produced from zona-free IVF embryos. We are pursuing this method as a prerequisite to developing viral vector co-culture delivery strategies.
Ritchie
William A
WA
Department of Gene Function and Development, Roslin Institute, Roslin, Midlothian, United Kingdom. william.ritchie@bbsrc.ac.uk
Taylor
Jane E
JE
Gardner
John O
JO
Wilmut
Ian
I
Carlisle
Ailsa
A
Neil
Claire
C
King
Tim
T
Whitelaw
C Bruce A
CB
D000818:Animals; D000831:Animals, Newborn; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D011247:Pregnancy; D012756:Sheep; D015044:Zona Pellucida / Q000502:physiology