The effect of maltose on dextran yield and molecular weight distribution.
Dextran synthesis has been studied since the Second World War, when it was used as blood plasma expander. This polysaccharide composed of glucose units is linked by an alpha-1,6-glucosidic bond. Dextransucrase is a bacterial extra cellular enzyme, which promotes the dextran synthesis from sucrose. When, besides sucrose, another substrate (acceptor) is also present in the reactor, oligosaccharides are produced and part of the glucosyl moieties from glucose is consumed to form these acceptor products, decreasing the dextran yield. Although dextran enzymatic synthesis has been extensively studied, there are few published studies regarding its molecular weight distribution. In this work, the effect of maltose on yield and dextran molecular weight synthesized using dextransucrase from Leuconostoc mesenteroides B512F, was investigated. According to the obtained results, maltose is not able to control and reduce dextran molecular weight distribution and synthesis carried out with or without maltose presented the same molecular weight distribution profile.
Rodrigues
Sueli
S
Departamento de Tecnologia de Alimentos, Universidade Federal do Ceará, Caixa Postal 12168, Campus do Pici, Bloco 858, 60021-970, Fortaleza, CE, Brazil. sueli@efftech.eng.br
Lona
Liliane M F
LM
Franco
Telma T
TT
D003911:Dextrans / Q000032:analysis* / Q000138:chemical synthesis*; D004789:Enzyme Activation; D005964:Glucosyltransferases / Q000737:chemistry*; D007934:Leuconostoc / Q000145:classification / Q000201:enzymology*; D008320:Maltose / Q000737:chemistry*; D013045:Species Specificity; D013395:Sucrose / Q000737:chemistry*
D003911:Dextrans; D013395:Sucrose; D008320:Maltose; D005964:Glucosyltransferases; C020025:dextransucrase