title: Diagnosis of congenital adrenal hyperplasia by rapid determination of 17alpha-hydroxyprogesterone in dried blood spots by gas chromatography/mass spectrometry following microwave-assisted silylation.
abstract: 17alpha-Hydroxyprogesterone (17OHP) is considered to be the biomarker of congential adrenal hyperplasia (CAH). Screening for CAH in newborns by measuring levels of the biomarker of 17OHP has become routine. In the work, a rapid, simple and sensitive technique was developed for the diagnosis of neonatal CAH by the quantitative analysis of 17OHP in neonatal blood spots. The technique was based on microwave-assisted silylation (MAS) followed by gas chromatography/mass spectrometry (GC/MS). In the method, fast derivatization of 17OHP with N,O-bis(trimethylsilyl)trifluoroacetamide was performed by using microwave irradiation, and the trimethylsilyl derivative thus formed was analyzed by GC/MS. The results of the experiment indicate that MAS followed by GC/MS analysis is a rapid, simple and sensitive method for the determination of 17OHP in blood samples. The proposed technique has been shown to have potential as a powerful tool for the rapid diagnosis of neonatal CAH.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200433, P.R.China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Jie
authors.initials: J
authors.lastname: Ji
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D019326:17-alpha-Hydroxyprogesterone / Q000097:blood* / Q000737:chemistry / Q000528:radiation effects; D000312:Adrenal Hyperplasia, Congenital / Q000097:blood* / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D001800:Blood Specimen Collection / Q000379:methods*; D003890:Desiccation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D008872:Microwaves; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry; D013048:Specimen Handling / Q000379:methods
chemical_list: D015415:Biomarkers; D019326:17-alpha-Hydroxyprogesterone; D012825:Silicon