Effective removal of albumin from serum.
The protein constituents of serum can range from grams to picograms per liter, making it technically difficult to achieve in-depth proteomic analysis. Removal of highly abundant proteins, such as albumin, coupled to powerful protein separation methods is required for increased sample load, thus facilitating detection and identification of low-abundant proteins. We report here a chemical-based extraction method for the effective and specific removal of albumin from serum.
Colantonio
David A
DA
Department of Physiology, Queen's University, Kingston, Ontario, Canada.
Dunkinson
Christy
C
Bovenkamp
Diane E
DE
Van Eyk
Jennifer E
JE
D000418:Albumins / Q000302:isolation & purification*; D000645:Ammonium Sulfate; D015415:Biomarkers / Q000097:blood; D001797:Blood Protein Electrophoresis / Q000379:methods*; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D044967:Serum / Q000737:chemistry*; D014227:Triazines
D000418:Albumins; D015415:Biomarkers; D014227:Triazines; C012842:Cibacron Blue F 3GA; D000645:Ammonium Sulfate