Spontaneous transfection of mammalian cells with plasmid DNA.
A simple method for spontaneous transfection into mammalian cells (both adherent and suspension in culture) with plasmid DNA is described. This method does not require any specific DNA carrier or technical device and can be applied for obtaining both transient and stably transfected cells. The efficiency of spontaneous transfection is slightly lower in comparison with that of the conventional calcium phosphate and lipofectin transfection methods and does not depend on the type of cell culture used.
Tsoncheva
Vania L
VL
Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.
Todorova
Kristina A
KA
Ivanov
Ivan G
IG
Maximova
Vera A
VA
D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids; D014162:Transfection / Q000379:methods
D004273:DNA, Neoplasm