title: Reproductive abnormalities in adult male mice following preimplantation exposures to estradiol or pesticide methoxychlor.
abstract: Adult females of ICR strain of mice were bred, separated into different experimental groups, and treated as follows. On Days 2-4 of pregnancy, the mice received daily subcutaneous injections of either 0.05 ml sesame oil (vehicle) or same volume of 5.0mg of purified methoxychlor (MXC) suspended in the vehicle. Another group received a single subcutaneous injection of 1.0 microg of estradiol-17beta (E) on Day 2 of pregnancy only. Male offspring were tested at 3 and 6 months of age. At 3 months, E or MXC did not alter the weights of seminal vesicles, preputial glands, or testes, although after exposure for 30 min to a female in estrus behind a partition, testosterone levels were significantly reduced in treated males in comparison to control males exposed to the same partition test. At 6 months, the preputial glands and testes weight remained unchanged, while the seminal vesicles were significantly heavier in E- and MXC-treated males. Same partition tests again revealed that in E and MXC groups, testosterone levels remained significantly lower in comparison to control males. MXC or E exposures during preimplantation appear to induce long-term effects on the sexual development in 3 and 6 month-old-males by compromising their sexual arousal and altering seminal vesicles weights in the older group.
authors.affiliation: Novosibirsk State University, Russia.
authors.forename: Sergei Ya
authors.initials: SY
authors.lastname: Amstislavsky
authors.forename: Tamara G
authors.initials: TG
authors.lastname: Amstislavskaya
authors.forename: Vjacheslav S
authors.initials: VS
authors.lastname: Amstislavsky
authors.forename: Marina A
authors.initials: MA
authors.lastname: Tibeikina
authors.forename: Kiril V
authors.initials: KV
authors.lastname: Osipov
authors.forename: Victor P
authors.initials: VP
authors.lastname: Eroschenko
mesh_terms: D000818:Animals; D001755:Blastocyst; D004958:Estradiol / Q000633:toxicity*; D005260:Female; D005837:Genitalia, Male / Q000473:pathology; D007306:Insecticides / Q000633:toxicity*; D008098:Litter Size / Q000187:drug effects; D008297:Male; D008731:Methoxychlor / Q000633:toxicity*; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012098:Reproduction / Q000187:drug effects*; D012669:Seminal Vesicles / Q000473:pathology; D012744:Sex Ratio; D012726:Sexual Behavior, Animal / Q000502:physiology; D013737:Testis / Q000473:pathology; D013739:Testosterone / Q000096:biosynthesis
chemical_list: D007306:Insecticides; D013739:Testosterone; D004958:Estradiol; D008731:Methoxychlor