Classification of microarrays to nearest centroids.
BACKGROUND
Classification of biological samples by microarrays is a topic of much interest. A number of methods have been proposed and successfully applied to this problem. It has recently been shown that classification by nearest centroids provides an accurate predictor that may outperform much more complicated methods. The 'Prediction Analysis of Microarrays' (PAM) approach is one such example, which the authors strongly motivate by its simplicity and interpretability. In this spirit, I seek to assess the performance of classifiers simpler than even PAM.


RESULTS
I surprisingly show that the modified t-statistics and shrunken centroids employed by PAM tend to increase misclassification error when compared with their simpler counterparts. Based on these observations, I propose a classification method called 'Classification to Nearest Centroids' (ClaNC). ClaNC ranks genes by standard t-statistics, does not shrink centroids and uses a class-specific gene-selection procedure. Because of these modifications, ClaNC is arguably simpler and easier to interpret than PAM, and it can be viewed as a traditional nearest centroid classifier that uses specially selected genes. I demonstrate that ClaNC error rates tend to be significantly less than those for PAM, for a given number of active genes.


BACKGROUND
Point-and-click software is freely available at http://students.washington.edu/adabney/clanc.
Dabney
Alan R
AR
Department of Biostatistics, University of Washington, Seattle, 98195, USA. adabney@u.washington.edu
D000465:Algorithms; D016000:Cluster Analysis; D003198:Computer Simulation; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007938:Leukemia / Q000235:genetics; D008223:Lymphoma / Q000235:genetics; D015233:Models, Statistical; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010363:Pattern Recognition, Automated; D012984:Software