Influence of leucine and valine on ramoplanin production by Actinoplanes sp. ATCC 33076.
Ramoplanin is a glycolipodepsipeptide antibiotic obtained by fermentation of the Actinoplanes sp. ATCC 33076, isolated as a complex of three closely related components A1, A2 and A3, which differ in their fatty acid moiety. We have investigated the influence of L-leucine and L-valine, the biosynthetic precursors of the fatty acids in A2 and A3 factors, on the complex composition and antibiotic productivity. Addition of 5 g/litre of L-leucine at the time of inoculation increases antibiotic production and improves the production of A2 factor, which represents the active principle component under clinical development. Addition of L-valine in the same conditions modifies the composition of the complex towards the A3 factor but does not improve total antibiotic productivity. A possible explanation for the different actions of the two amino acids is presented.
Brunati
Mara
M
Vicuron Pharmaceuticals, Via R Lepetit 34, 21040, Gerenzano, Varese, Italy. mbrunati@vicuron.it
Bava
Adriana
A
Marinelli
Flavia
F
Lancini
Giancarlo
G
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D047630:Depsipeptides / Q000096:biosynthesis* / Q000737:chemistry; D005285:Fermentation / Q000187:drug effects*; D007930:Leucine / Q000494:pharmacology*; D008848:Micromonosporaceae / Q000187:drug effects* / Q000378:metabolism; D015394:Molecular Structure; D014633:Valine / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D047630:Depsipeptides; C041438:ramoplanin; D007930:Leucine; D014633:Valine