title: Blood culture and conventional media for vitreous culture in infectious endophthalmitis.
abstract: OBJECTIVE
  To study culture of vitreous fluid specimens in patients with infectious endophthalmitis, using blood culture bottles compared with conventional culture media.
  
  
  METHODS
  Patients with infectious endophthalmitis, occurred within 6 weeks after ocular trauma or intraocular surgery, were prospectively studied. Vitreous fluid specimens were cultured in both blood culture bottles and conventional culture media. The measured outcome is the yield of positive culture and time to positive culture.
  
  
  RESULTS
  The vitreous fluid culture was positive in 14 of 27 eyes (51.85%). Blood culture bottle was positive in 14 of 14 eyes (100%), whereas conventional culture media was positive in 7 of 14 eyes (50%). Most specimens in both techniques showed positive culture within 24 hours.
  
  
  CONCLUSIONS
  Vitreous fluid culture with blood culture bottles is superior to conventional media with statistically significant difference. There was no significant difference in time to positive culture.
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. yosanan@kku.ac.th
authors.forename: Yosanan
authors.initials: Y
authors.lastname: Yospaiboon
authors.forename: Sarawuth
authors.initials: S
authors.lastname: Saree
authors.forename: Sirichai
authors.initials: S
authors.lastname: Pasadhika
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D009877:Endophthalmitis / Q000175:diagnosis* / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D014822:Vitreous Body / Q000382:microbiology*
chemical_list: D003470:Culture Media