title: Dye coupling in Purkinje cells of organotypic slice cultures.
abstract: Cerebellar slice cultures of newborn rats showed poorly developed dendritic arborization of Purkinje cells, whereas cultures of 10-day-old rats revealed prominent dendritic branching. Gap junctional intercellular communication between Purkinje cells, investigated as dye transfer of microinjected neurobiotin, occurred through dendro-dendritic contacts, with decreased dye spreading in old cell cultures. These results indicate a possible correlation of gap junctional intercellular communication and the development of Purkinje cells.
authors.affiliation: Medizinische Fakultät, Institut für Anatomie, Abteilung für Cytologie, Ruhr-Universität Bochum, Universitätsstrasse 150, D-44780 Bochum, Germany.
authors.forename: Karl
authors.initials: K
authors.lastname: Meller
authors.forename: Kerstin
authors.initials: K
authors.lastname: Krah
authors.forename: Carsten
authors.initials: C
authors.lastname: Theiss
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001710:Biotin / Q000031:analogs & derivatives; D002450:Cell Communication / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002463:Cell Membrane Permeability / Q000502:physiology; D002525:Cerebellar Cortex / Q000254:growth & development* / Q000648:ultrastructure; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure; D004058:Diffusion; D005614:Freeze Fracturing; D017629:Gap Junctions / Q000502:physiology*; D008845:Microinjections / Q000379:methods; D009924:Organ Culture Techniques / Q000379:methods; D011689:Purkinje Cells / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D013194:Staining and Labeling / Q000379:methods
chemical_list: C070614:neurobiotin; D001710:Biotin