Effect of magnetic field on the fibronectin adsorption, cell attachment and proliferation on titanium surface.
OBJECTIVE
We studied the effect of various static magnetic fields (SMFs) on the adsorption of specific recombinant fibronectin (FN) peptide (hFNIII9-10) on the titanium surface. Furthermore, the responses of human osteosarcoma TE-85 cells in the SMF were observed.


METHODS
Various magnetic fields--1, 2, 3, 5, 7, 10 mT--were established by controlling the distance from Nd-Fe-B magnet to the disks. For FN adsorption experiment, machined titanium disks were incubated in 1 microM hFNIII9-10 at 37 degrees C overnight under magnetic field. The adsorbed hFNIII9-10 was measured as optical density (OD). For attachment study, TE-85 cells were incubated for 2 h on the hFNIII9-10 coated machined titanium disks and OD values were measured. As for proliferation study, titanium disks were incubated for 48 h after washing unattached cells in 2 h. The amount of proliferated TE-85 cell was also measured as OD value. Attachments of TE-85 cells under various intensities of magnetic field were observed using a scanning electron microscope.


RESULTS
The amount of adsorbed hFNIII9-10 showed no significant difference between control (0 mT) and six experimental groups (1, 2, 3, 5, 7, 10 mT). However, TE-85 cells attached significantly higher in groups of 1, 2, 5, 10 mT than in control group (P=0). Cell attachment in groups of 3, 7 mT showed no significant difference with that of control group. TE-85 cells were observed to attach through filopodia. Especially in 1 mT, flattened cells were predominant. In proliferation assay, 1 mT stimulated TE-85 cells showed significantly higher proliferation than those in 2, 3 and 7 mT (P=0).


CONCLUSIONS
Magnetic fields under 10 mT did not influence FN adsorption on the titanium surface. However, a significant effect was found on cell attachment and proliferation.
Kim
Hyo Jin
HJ
Department of Prosthodontics, College of Dentistry, Seoul National University, Seoul, South Korea.
Chang
Ik Tae
IT
Heo
Seong Joo
SJ
Koak
Jai Young
JY
Kim
Seong Kyun
SK
Jang
Jun Hyeog
JH
D000327:Adsorption; D000704:Analysis of Variance; D049109:Cell Proliferation; D005353:Fibronectins / Q000493:pharmacokinetics*; D006801:Humans; D011827:Radiation; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
D005353:Fibronectins; C501364:fibronectin type III like peptide, human; D014025:Titanium