title: The immunohistochemical profile of the primary and metastatic carcinoma of the clitoris: a case report.
abstract: We describe the immunohistochemical profile of rare primary squamous carcinoma of the clitoris metastasizing to the bilateral inguinal lymph nodes. Several antigens were assessed immunohistochemically (pRb1, p16INK4A, cyclin D1, cdk4, estrogen receptor (ER), progesterone receptor (PR), androgen receptor (AR), p53, Ki-67, p27KIP1, PTEN, hMLh1, phospho-AKT, collagen IV, leptin and CD90) in both tumors. All the antibodies applied revealed a staining pattern that is typical of primary and metastatic carcinomas. Cyclin D1-cdk4 complex was overexpressed, whereas there was no p16INK4A immunostaining. Moreover, both tumors expressed positivity for p53 protein, but were negative for estrogen and progesterone receptors. The proliferative activity of cancer, assessed by MIB-1 Proliferative Index, amounted to 25% either for primary or for metastatic tumors. As a conclusion, immunohistochemical assessment of various cell-cycle-associated molecules yield clues as to their possible function during the process of spread of rare neoplasm originating from the clitoris.
authors.affiliation: IInd Department of Gynecology, Lublin University School of Medicine, 8 Jaczewski str, 20-954, Lublin, Poland. andrzej.semczuk@am.lublin.pl
authors.forename: Andrzej
authors.initials: A
authors.lastname: Semczuk
authors.forename: Danuta
authors.initials: D
authors.lastname: Skomra
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Jankiewicz
authors.forename: Aneta
authors.initials: A
authors.lastname: Adamiak
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Korobowicz
authors.forename: Tomasz
authors.initials: T
authors.lastname: Rechberger
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000556:secondary / Q000601:surgery; D002987:Clitoris / Q000473:pathology*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D014846:Vulvar Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery