title: Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae.
abstract: Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3Delta1. Leaky expression was eliminated using 3-aminotriazole and gene conversion was eliminated by using the Schizosaccharomyces pombe his5+ gene, resulting in a 5- to 10-fold improvement in the efficiency of SGA.
authors.affiliation: Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210-2339, USA.
authors.forename: Jewel A
authors.initials: JA
authors.lastname: Daniel
authors.forename: Jiyoun
authors.initials: J
authors.lastname: Yoo
authors.forename: Blaine T
authors.initials: BT
authors.lastname: Bettinger
authors.forename: David C
authors.initials: DC
authors.lastname: Amberg
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Burke
mesh_terms: D000640:Amitrole / Q000378:metabolism*; D015825:Chromosomes, Fungal; D005656:Fungal Proteins / Q000235:genetics*; D005785:Gene Conversion; D016681:Genome, Fungal; D006639:Histidine / Q000235:genetics*; D046228:Microarray Analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D006639:Histidine; D000640:Amitrole