[Prolonged stimulation of hematopoiesis in mice with chemotactic peptide N-f-met-leu-phe-gly incorporated into liposomes].
The experimental study of macrophage-activating chemotactic peptide N-f-met-leu-phe-gly (chemotaxic peptide), as well as its liposomal form, on the proliferation and migration of colony-forming precursor cells in mice was carried out. The study revealed that the subcutaneous injection of chemotaxic peptide into mice in a dose of 20 Mg led to a pronounced, but short-term increase in the proliferation of such precursor cells in the marrow: as shown by the hydroxyurea "suicide" method, a day after the injection almost 50% of hematopoietic stem cells entered the S-phase of the cellular cycle; in addition, an increase in the content of colony-forming precursor cells in the peripheral blood and the spleen was observed. The injection of chemotaxic peptide, incapsulated into liposomes, led to a considerable increase in the duration of the stimulating effect, manifested by the maintenance of a stable proliferative state of the pool of hematopoietic stem cells during 4 weeks (the term of observation). This effect could be attributed to the formation of the liposomal "depot" and the gradual liberation of chemotaxic peptide from it.
Sanin
A V
AV
Sosnovskaia
O Iu
OIu
Kozhevnikova
T N
TN
Golovanova
T A
TA
Medvedkin
V N
VN
Kashkin
K P
KP
D000818:Animals; D020296:Animals, Congenic; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis / Q000187:drug effects*; D007279:Injections, Subcutaneous; D008081:Liposomes / Q000008:administration & dosage / Q000494:pharmacology*; D008264:Macrophages; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors; D014611:Vaccination
D008081:Liposomes; D009240:N-Formylmethionine Leucyl-Phenylalanine