Determination of residual clopidol in chicken muscle by capillary gas chromatography/mass spectrometry.
Clopidol was extracted from chicken with acetonitrile, and the extract was evaporated to about 5 mL. The concentrated solution was centrifuged and applied to a glass column filled with basic alumina. The column was eluted with methanol and the methanol was evaporated to dryness. The residue was derivatized with Sylon BFT and analyzed by gas chromatography/mass spectrometry (GC/MS). Mass characteristics of trimethylsilyl derivative of clopidol were interpreted manually. The selected-ion monitoring mode was performed at m/z 191, 248, 263, and 265. A sensitive, reproducible GC/MS method was developed for monitoring residues of clopidol in chickens. All recoveries of the drug from chicken muscle fortified at 1, 10, and 100 ng/g were > 65%, and relative standard deviations were < 15%. The detection limit was about 0.5 ng/g, and the quantitation limit was the lower limit of the standard curve, which was about 1 ng/g.
He
Limin
L
South China Agricultural University, Centre for Veterinary Drug Residues, College of Veterinary Medicine, Guangzhou, Guangdong, PC 510642, People's Republic of China. liminokhe@scau.edu.cn
Liu
Yahong
Y
Zeng
Zhenling
Z
Huang
Xianhui
X
Liu
Rong
R
D000537:Aluminum Oxide / Q000032:analysis; D000818:Animals; D002645:Chickens; D003007:Clopidol / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007477:Ions; D000432:Methanol / Q000032:analysis; D008956:Models, Chemical; D009132:Muscles / Q000378:metabolism*; D015203:Reproducibility of Results; D014297:Trimethylsilyl Compounds / Q000032:analysis
D007477:Ions; D014297:Trimethylsilyl Compounds; D003007:Clopidol; D000537:Aluminum Oxide; D000432:Methanol; C055926:trimethylsilanol