title: Novel probes for protein chip applications.
abstract: Protein microarrays (protein chips), due to their high throughput, have great potential to reduce the cycle time of drug discovery and to improve the efficiency of medical diagnostics. A great deal of effort has been made to facilitate the development of protein chips, such as the antibody microarrays. High-throughput fabrication of purified high-affinity probes is now one of the bottlenecks for protein microarrays. We discuss here three novel strategies to fabricate probes, namely, single chain antibodies displayed by phage, protein-oligonucleotide conjugates, and aptamers, which are promising in the protein chip technology.
authors.affiliation: Department of Chemical Biology, College of Chemistry, Peking University, Beijing 100871, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liao
authors.forename: Su
authors.initials: S
authors.lastname: Guo
authors.forename: Xin Sheng
authors.initials: XS
authors.lastname: Zhao
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques; D019295:Computational Biology; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D008027:Light; D008954:Models, Biological; D009841:Oligonucleotides / Q000737:chemistry*; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods*; D011487:Protein Conformation; D025941:Protein Interaction Mapping / Q000379:methods; D011506:Proteins / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods; D013499:Surface Properties
chemical_list: D000906:Antibodies; D007128:Immunoglobulin Fragments; D009841:Oligonucleotides; D019151:Peptide Library; D011506:Proteins; D020543:Proteome