[Production of transgenic pea (Pisum sativum L.) plants resistant to the herbicide pursuit].
Transgenic pea (Pisum sativum L.) plants containing mutant ahas/als gene were obtained using Agrobacterium-mediated genetic transformation. Transformation has been carried out using cocultivation of pea explants with Agrobacterium tumefaciens strain lBA4404 carrying genetic vectors pCB004, pCB006 and pCB007 containing ahas/als and nptII genes. The presence of transferred genes in the genomes of transgenic plants has been confirmed by PCR analysis.
Nifantova
S N
SN
Simonenko
Iu V
IuV
Komarnitskiĭ
I K
IK
Kuchuk
N V
NV
D004351:Drug Resistance / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D006540:Herbicides; D009154:Mutation; D009539:Nicotinic Acids; D018532:Pisum sativum / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
D006540:Herbicides; D009539:Nicotinic Acids; C093630:imazethapyr