title: Identification of a 1.1-Mb region for a carcass weight QTL onbovine Chromosome 14.
abstract: We previously mapped a quantitative trait locus for carcass weight, designated Carcass Weight-1 (CW-1), to bovine Chromosome 14 using a purebred Wagyu pedigree based on progeny design analysis. To refine the critical region within 8.1 cM flanked by microsatellites BMS1941 and INRA094, we constructed a bacterial artificial chromosome (BAC) contig composed of 60 tiled BAC clones and prepared a high-density physical map including 80 microsatellites, of which 55 were developed in this study. We conducted linkage disequilibrium (LD) mapping in the CW-1 region with 47 microsatellites using paternal half-sib pedigrees whose sires exhibited homozygous CW-1 Q alleles in the region. The LD mapping study significantly narrowed the CW-1 locus to the 1.1-Mb region between microsatellites DIK7012 and DIK7020. Finally, we surveyed the 1.1-Mb-region genotypes of 1700 steers from 11 bulls having -/-, Q/-, or Q/Q alleles in the region, and we examined the effect of the CW-1 Q allele on carcass weight. The presence of the first Q increased carcass weight by 23.6 kg (95% confidence interval [CI], 17.6-29.5 kg), and the second Q increased carcass weight an additional 15.2 kg (95% CI, 10.7-19.7 kg). These results indicate the presence of a gene responsible for carcass weight within the 1.1-Mb region.
authors.affiliation: Cattle Breeding Development Institute of Kagoshima Prefecture, Tsukino, Osumi, So, Kagoshima 899-8212, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Mizoshita
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takano
authors.forename: Toshio
authors.initials: T
authors.lastname: Watanabe
authors.forename: Akiko
authors.initials: A
authors.lastname: Takasuga
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Sugimoto
mesh_terms: D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D033481:Chromosomes, Mammalian / Q000235:genetics*; D017931:DNA Primers; D015810:Linkage Disequilibrium; D008460:Meat; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers