Reducing the expression of glutathione transferase D mRNA in Drosophila melanogaster exposed to phenol and aniline.
Phenol and aniline are toxic to animals. The purpose of the present study was to examine the expression of glutathione transferase D mRNA in fruit flies altered by long-term exposure to phenol and aniline. Changes in the amount of mRNA were measured by a semiquantitative reverse-transcription polymerase chain reaction assay. The level of each glutathione transferase D mRNA expressed in the phenol-treated and aniline-treated strains of adult fruit flies differed after chemical treatment. Aniline was more potent than phenol in suppressing the expression of cytosolic glutathione transferase D mRNA. Aniline reduced the level of glutathione transferase mRNA expressed in the aniline-treated strain to less than a 0.5 fraction as compared to that measured in the wild-type strain. But phenol was only able to suppress the GstD7 and GstD4 mRNAs expressed in the phenol-treated strain. Neither aniline nor phenol reduced the expression of microsomal glutathione transferase mRNA in fruit flies.
Ding
K
K
Department of Biology, National Changhua University of Education, Changhua 500, Taiwan.
Chien
Y
Y
Chien
C
C
D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D003600:Cytosol / Q000378:metabolism; D015536:Down-Regulation; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000201:enzymology; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D019800:Phenol / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism
D000814:Aniline Compounds; D002273:Carcinogens; D029721:Drosophila Proteins; D007527:Isoenzymes; D012333:RNA, Messenger; D019800:Phenol; C410516:GstE1 protein, Drosophila; D005982:Glutathione Transferase; C023650:aniline