title: Functionality and stability of heparin immobilized onto poly(dimethylsiloxane).
abstract: Poly(dimethylsiloxane) (PDMS) has become an attractive material when working in the field of microfluidics, mainly because of the rapid prototyping process it involves. The increased surface volume ratio in microchannels makes the interaction between sample and material surface highly important, evident when handling complex biological samples such as plasma or blood. This study demonstrates a new grade of non-covalent heparin surface that adds efficient anticoagulant property to the PDMS material. The surface modification is a simple and fast one-step process performed at neutral pH, optimal when working with closed microsystems. The heparin formed a uniform and functional coating on hydrophobic PDMS with comparatively high level of antithrombin-binding capacity. In addition, long-term studies revealed that the immobilized heparin was more or less stable in the microchannels over a time of three weeks. Recalcified plasma in contact with native PDMS showed complete coagulation after 1h, while no fibrin formation was detected in plasma incubated on heparin-coated PDMS within the same time. In conclusion, we see the heparin coating developed and evaluated in this study as a tool that greatly facilitates the use of PDMS in microfluidics dealing with plasma or blood samples.
authors.affiliation: Department of Engineering Sciences, Angström Laboratory, Uppsala University, Box 534, SE-751 21 Uppsala, Sweden.
authors.forename: Sara
authors.initials: S
authors.lastname: Thorslund
authors.forename: Javier
authors.initials: J
authors.lastname: Sanchez
authors.forename: Rolf
authors.initials: R
authors.lastname: Larsson
authors.forename: Fredrik
authors.initials: F
authors.lastname: Nikolajeff
authors.forename: Jonas
authors.initials: J
authors.lastname: Bergquist
mesh_terms: D000991:Antithrombins; D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D005337:Fibrin; D006493:Heparin; D006801:Humans; D012828:Silicones
chemical_list: D000991:Antithrombins; D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon; D005337:Fibrin; D006493:Heparin