Transport activity of MCT1 expressed in Xenopus oocytes is increased by interaction with carbonic anhydrase.
Injection of carbonic anhydrase isoform II (CA) into Xenopus frog oocytes increased the rate of H+ flux via the rat monocarboxylate transporter isoform 1 (MCT1) expressed in the oocytes. MCT1 activity was assessed by changes of intracellular H+ concentration measured by pH-selective microelectrodes during application of lactate. CA-induced augmentation of the rate of H+ flux mediated by MCT1 was not inhibited by ethoxyzolamide (10 microM) and did not depend on the presence of added CO2/HCO3- but was suppressed by injection of an antibody against CA. Deleting the C terminus of the MCT1 greatly reduced its transport rate and removed transport facilitation by CA. Injected CA accelerated the CO2/HCO3(-)-induced acidification severalfold, which was blocked by ethoxyzolamide and was independent of MCT1 expression. Mass spectrometry confirmed activity of CA as injected into the frog oocytes. With pulldown assays we demonstrated a specific binding of CA to MCT1 that was not attributed to the C terminus of MCT1. Our results suggest that CA enhances MCT1 transport activity, independent of its enzymatic reaction center, presumably by binding to MCT1.
Becker
Holger M
HM
Abteilungen, Allgemeine Zoologie, Fachbereich Biologie, Technische Universität Kaiserslautern, D-67653 Kaiserslautern, Germany.
Hirnet
Daniela
D
Fecher-Trost
Claudia
C
Sültemeyer
Dieter
D
Deitmer
Joachim W
JW
D000818:Animals; D001692:Biological Transport; D002021:Buffers; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D004566:Electrodes; D004594:Electrophysiology; D004912:Erythrocytes / Q000201:enzymology; D005016:Ethoxzolamide / Q000494:pharmacology; D017353:Gene Deletion; D005978:Glutathione / Q000737:chemistry; D005982:Glutathione Transferase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013058:Mass Spectrometry; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism* / Q000502:physiology*; D009865:Oocytes / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011522:Protons; D018075:RNA, Complementary / Q000378:metabolism; D051381:Rats; D012685:Sepharose / Q000737:chemistry / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000378:metabolism; D027981:Symporters / Q000378:metabolism* / Q000502:physiology*; D013997:Time Factors; D014981:Xenopus; D000099233:Monocarboxylate Transport Protein 1
D002021:Buffers; D002256:Carbonic Anhydrases; D005016:Ethoxzolamide; D005978:Glutathione; D005982:Glutathione Transferase; D027501:Monocarboxylic Acid Transporters; D011522:Protons; D018075:RNA, Complementary; D012685:Sepharose; D012710:Serum Albumin, Bovine; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1