title: Hemoglobin recognition by imprinting in semi-interpenetrating polymer network hydrogel based on polyacrylamide and chitosan.
abstract: Semi-interpenetrating polymer network (semi-IPN) hydrogel was prepared to recognize hemoglobin, by molecularly imprinted method, in the mild aqueous media of chitosan and acrylamide in the presence of N,N'-methylenebisacrylamide as the cross-linking agent. The hydrogel obtained has been investigated by using thermal analysis, X-ray diffraction, differential scanning calorimetry (DSC), and environmental scanning electron microscope (ESEM). Langmuir analysis showed that an equal class of adsorption was formed in the hydrogel, and the adsorption equilibrium constant and the maximum adsorption capacity were evaluated to be 4.27 g/mL and 36.53 mg/g wet hydrogel, respectively. The imprinted semi-IPN hydrogel has a much higher adsorption capacity for hemoglobin than the nonimprinted hydrogel with the same chemical composition and also has a higher selectivity for the imprinted molecule.
authors.affiliation: State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin, China, 300071.
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Xia
authors.forename: Tian-Ying
authors.initials: TY
authors.lastname: Guo
authors.forename: Mou-Dao
authors.initials: MD
authors.lastname: Song
authors.forename: Bang-Hua
authors.initials: BH
authors.lastname: Zhang
authors.forename: Bao-Long
authors.initials: BL
authors.lastname: Zhang
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001704:Biopolymers / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D013696:Temperature; D013818:Thermogravimetry; D014867:Water; D014961:X-Ray Diffraction; D000099166:Polyacrylamides
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D001672:Biocompatible Materials; D001704:Biopolymers; D048271:Chitosan; D006454:Hemoglobins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020100:Hydrogels; D011108:Polymers; D014867:Water; D000099166:Polyacrylamides; C021221:N,N'-methylenebisacrylamide