title: Rapid identification of Staphylococcus aureus in blood cultures by a combination of fluorescence in situ hybridization using peptide nucleic acid probes and flow cytometry.
abstract: Fluorescence in situ hybridization (FISH) using peptide nucleic acid probes (PNAs) allows the identification of Staphylococcus aureus from human blood culture samples. We present data revealing that the combination of PNA FISH and flow cytometry is a possible approach for the noncultural identification of staphylococci in blood cultures.
authors.affiliation: Institut für Medizinische Mikrobiologie und Hygiene, Tübingen, Germany.
authors.forename: Hanna
authors.initials: H
authors.lastname: Hartmann
authors.forename: Henrik
authors.initials: H
authors.lastname: Stender
authors.forename: Andrea
authors.initials: A
authors.lastname: Schäfer
authors.forename: Ingo B
authors.initials: IB
authors.lastname: Autenrieth
authors.forename: Volkhard A J
authors.initials: VA
authors.lastname: Kempf
mesh_terms: D016470:Bacteremia / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids / Q000235:genetics*; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D003470:Culture Media; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids