title: Cytogenetic effects of commercial formulation of cypermethrin in root meristem cells of Allium sativum: spectroscopic basis of chromosome damage.
abstract: To validate the use of Allium sativum as a sensitive test model for genotoxicity, the cytogenetic effects of a commercial formulation of the pyrethroid insecticide, cypermethrin, were evaluated in the root meristem cells of A. sativum. Ultraviolet (UV) and Fourier transform infrared (FTIR) spectral measurements were also carried out to understand the interaction of cypermethrin with DNA. In a preliminary toxicity assay, the EC50 for Allium root growth was estimated to be 8 ppm. For the cytogenetic assay, root meristem cells were exposed to 1, 2, 4, 8 and 16 ppm of the test compound for 24 h, and either processed immediately for analysis or incubated in water for 24 h of recovery and then processed. Cells analyzed immediately after the exposure had a significant, dose-dependent inhibition of mitotic index (MI) and induction of mitotic and chromosomal aberrations (MAs and CAs). The 24 h recovery period reduced the effect of the test compound on the MI and percent aberrations; however, cells exposed to 8 and 16 ppm showed a significant frequency of aberrations despite the recovery period. One part per million cypermethrin was consistently negative in the assay. The data indicate that higher doses of cypermethrin produce toxicity, CAs and MAs in A. sativum. The present study indicates that A. sativum is a sensitive and reliable test system. A bathochromic shift observed in UV absorption spectra reveals that cypermethrin binds with DNA. Role of vibrational modes of the active site in the recognition and reaction of cypermethrin with DNA has been discussed. Based on spectroscopic data and structural properties, a possible mechanism has been proposed for the interaction of cypermethrin with DNA resulting in chromosomal aberrations.
authors.affiliation: Cell Biology Division, Industrial Toxicology Research Centre, P.O. Box No. 80, M.G. Road, Lucknow 226001, India.
authors.forename: P N
authors.initials: PN
authors.lastname: Saxena
authors.forename: L K S
authors.initials: LK
authors.lastname: Chauhan
authors.forename: S K
authors.initials: SK
authors.lastname: Gupta
mesh_terms: D002869:Chromosome Aberrations / Q000139:chemically induced / Q000187:drug effects; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005737:Garlic / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D007306:Insecticides / Q000737:chemistry / Q000633:toxicity*; D018519:Meristem / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D008940:Mitotic Index; D009152:Mutagenicity Tests / Q000379:methods; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D011722:Pyrethrins / Q000737:chemistry / Q000633:toxicity*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013997:Time Factors
chemical_list: D007306:Insecticides; D011722:Pyrethrins; C017160:cypermethrin; D004247:DNA