Comparison of a dot immunobinding assay and the serum agglutination test for detecting serological responses in vaccinated and unvaccinated chickens following challenge with Salmonella gallinarum.
A dot immunobinding assay (DIA) was compared with the standard tube agglutination test (SAT) for detecting antibodies in sera from vaccinated and unvaccinated chickens challenged with Salmonella gallinarum var duisburg . The virulence plasmid-cured S. gallinarum vaccine was given at 7 and 21 days, and the challenge at 35 days. The DIA was very sensitive and detected antibodies to high titres in challenged birds. It also detected high titres in vaccinated, unchallenged birds which failed to react by SAT.
Madhuri
D
D
Department of Veterinary Pathology, CCS Haryana Agricultural University, Hisar, India. hau@hau.ren.nic.in
Chand
P
P
Sadana
J R
JR