Molecular and genetic features of a labeled class of spinal substantia gelatinosa neurons in a transgenic mouse.
Genetic incorporation in a mouse of a transgene containing the prion promoter and the green fluorescent protein (GFP) coding sequence labels a set of substantia gelatinosa (SG) neurons (SG-GFP) homogenous in morphology, electrophysiology, and gamma-amino-butyric acid expression. In the present analysis the SG-GFP neurons are established to have protein kinase C-betaII immunoreactivity and to lack evidence for the presence of calbindin D-28k, parvalbumin, and protein kinase C-gamma. These neurons were hyperpolarized by mediators of descending control, norepinephrine and serotonin. Sequential polymerase chain reactions established the insertion of the transgene to be in the receptor protein tyrosine phosphatase kappa (RPTP-kappa) and the laminin receptor 1 (ribosomal protein SA) pseudogene 1 locus. RPTP-kappa expression in both GFP-labeled dorsal root ganglia and SG neurons raises the possibility that homophilic interactions of RPTP-kappa contribute to establishment of connections between specific classes of primary afferent and SG neurons.
Hantman
Adam W
AW
Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
Perl
Edward R
ER
D000818:Animals; D064026:Calbindins; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000145:classification / Q000166:cytology* / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D064546:Protein Kinase C beta; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D013194:Staining and Labeling; D013376:Substantia Gelatinosa / Q000166:cytology* / Q000378:metabolism; D014018:Tissue Distribution
D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D049452:Green Fluorescent Proteins; C087857:protein kinase C gamma; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D017027:Protein Tyrosine Phosphatases; C080778:Ptprk protein, mouse; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2