Morphological and molecular analysis of idiopathic gingival fibromatosis: a case report.
OBJECTIVE
We analyse a case of idiopathic gingival overgrowth using morphological and molecular methods. As this overgrowth involves collagen accumulation in the gingival connective tissue, we measured the collagen turnover to clarify the pathogenic mechanisms potentially involved.


METHODS
The patient was a 29-year-old Italian woman with enlargement of the gingivae throughout the entire mandible and maxilla. Morphological analyses were carried out on haematoxylin-eosin and Sirius red-stained paraffin-embedded gingival sections. mRNA levels of collagen type I and III, matrix metalloproteinase (MMP)-1, transforming growth factor-beta1 and lysyl hydroxylase (LH)2b were determined by RT-PCR on cultured gingival fibroblasts and compared with healthy control fibroblasts. Interstitial collagen and MMP-1 content in the supernatants were assessed, respectively, by dot blot and SDS zymography.


CONCLUSIONS
In Sirius red-stained sections of the patient's overgrown gingivae, interstitial collagen content was 29% higher than controls. Her gingival fibroblasts had higher collagen type I, MMP-1 and LH2b gene expression and unmodified interstitial collagen, type I protein levels in the supernatants. These findings would seem to suggest that in this case collagen accumulation in the gingival connective tissue was not associated with increased synthesis and decreased degradation.
Gagliano
Nicoletta
N
Department of Human Morphology-LITA Segrate, University of Milan, Milan, Italy. nicoletta.gagliano@unimi.it
Moscheni
Claudia
C
Dellavia
Claudia
C
Masiero
Silvia
S
Torri
Carlo
C
Grizzi
Fabio
F
Stabellini
Giordano
G
Gioia
Magda
M
D000328:Adult; D003094:Collagen / Q000032:analysis; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D005351:Fibromatosis, Gingival / Q000201:enzymology* / Q000473:pathology; D005881:Gingiva / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000032:analysis
D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D003094:Collagen