Defined DNA/nanoparticle conjugates.
Glutathione monolayer-protected gold clusters were reacted by place exchange with 19- or 20-residue thiolated oligonucleotides. The resulting DNA/nanoparticle conjugates could be separated on the basis of the number of bound oligonucleotides by gel electrophoresis and assembled with one another by DNA-DNA hybridization. This approach overcomes previous limitations of DNA/nanoparticle synthesis and yields conjugates that are precisely defined with respect to both gold and nucleic acid content.
Ackerson
Christopher J
CJ
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Sykes
Michael T
MT
Kornberg
Roger D
RD
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005978:Glutathione / Q000737:chemistry; D017979:Gold Colloid / Q000138:chemical synthesis / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010316:Particle Size
D017979:Gold Colloid; D009841:Oligonucleotides; D005978:Glutathione