Desulfotomaculum carboxydivorans sp. nov., a novel sulfate-reducing bacterium capable of growth at 100% CO.
A moderately thermophilic, anaerobic, chemolithoheterotrophic, sulfate-reducing bacterium, strain CO-1-SRB(T), was isolated from sludge from an anaerobic bioreactor treating paper mill wastewater. Cells were Gram-positive, motile, spore-forming rods. The temperature range for growth was 30-68 degrees C, with an optimum at 55 degrees C. The NaCl concentration range for growth was 0-17 g l(-1); there was no change in growth rate until the NaCl concentration reached 8 g l(-1). The pH range for growth was 6.0-8.0, with an optimum of 6.8-7.2. The bacterium could grow with 100% CO in the gas phase. With sulfate, CO was converted to H(2) and CO(2) and part of the H(2) was used for sulfate reduction; without sulfate, CO was completely converted to H(2) and CO(2). With sulfate, strain CO-1-SRB(T) utilized H(2)/CO(2), pyruvate, glucose, fructose, maltose, lactate, serine, alanine, ethanol and glycerol. The strain fermented pyruvate, lactate, glucose and fructose. Yeast extract was necessary for growth. Sulfate, thiosulfate and sulfite were used as electron acceptors, whereas elemental sulfur and nitrate were not. A phylogenetic analysis of 16S rRNA gene sequences placed strain CO-1-SRB(T) in the genus Desulfotomaculum, closely resembling Desulfotomaculum nigrificans DSM 574(T) and Desulfotomaculum sp. RHT-3 (99 and 100% similarity, respectively). However, the latter strains were completely inhibited above 20 and 50% CO in the gas phase, respectively, and were unable to ferment CO, lactate or glucose in the absence of sulfate. DNA-DNA hybridization of strain CO-1-SRB(T) with D. nigrificans and Desulfotomaculum sp. RHT-3 showed 53 and 60% relatedness, respectively. On the basis of phylogenetic and physiological features, it is suggested that strain CO-1-SRB(T) represents a novel species within the genus Desulfotomaculum, for which the name Desulfotomaculum carboxydivorans is proposed. This is the first description of a sulfate-reducing micro-organism that is capable of growth under an atmosphere of pure CO with and without sulfate. The type strain is CO-1-SRB(T) (=DSM 14880(T)=VKM B-2319(T)).
Parshina
Sofiya N
SN
Laboratory of Microbiology of Anthropogenic Environments, Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60 let Oktyabrya 7 b. 2, Moscow, Russia.
Sipma
Jan
J
Sub-Department of Environmental Technology, Wageningen University, Bomenweg 2, PO Box 8129, 6700 EV Wageningen, The Netherlands.
Nakashimada
Yutaka
Y
Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
Henstra
Anne Meint
AM
Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
Smidt
Hauke
H
Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
Lysenko
Anatoly M
AM
Laboratory of Microbiology of Anthropogenic Environments, Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60 let Oktyabrya 7 b. 2, Moscow, Russia.
Lens
Piet N L
PNL
Sub-Department of Environmental Technology, Wageningen University, Bomenweg 2, PO Box 8129, 6700 EV Wageningen, The Netherlands.
Lettinga
Gatze
G
Sub-Department of Environmental Technology, Wageningen University, Bomenweg 2, PO Box 8129, 6700 EV Wageningen, The Netherlands.
Stams
Alfons J M
AJM
Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
D000693:Anaerobiosis; D019149:Bioreactors; D002248:Carbon Monoxide / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D045859:Desulfotomaculum / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010209:Paper; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal / Q000379:methods; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013431:Sulfates / Q000378:metabolism*; D016999:Sulfur-Reducing Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013431:Sulfates; D002248:Carbon Monoxide