[Effects of murine bone marrow endothelial cells on the in vitro expansion of human cord blood hematopoietic progenitor cells].
OBJECTIVE
To observe the effects of bone marrow endothelial cells on the expansion of human umbilical cord blood (UCB) hematopoietic progenitors in vitro.


METHODS
Bone marrow endothelial cells were cultured and the serum free conditioned media (ECM) were collected. CD34+ cells derived from human umbilical cord blood (UCB) were isolated by MiniMACS. Hematopoietic progenitor cells were assayed after culturing with ECM or co-culturing with endothelial cell layer (ECL) for 24 hours. The effects of conditioned medium of endothelial cells which were cultured with bFGF (bFGF-ECM) on the expansion of cord blood hematopoietic progenitors also were investigated.


RESULTS
UCB hematopoietic progenitors were significantly expanded by adding ECM or co-cultured with ECL, and ECM cultures and ECL cultures showed similar expansion effect on early progenitor cells. The effect of bFGF-ECM on the expansion of UCB hematopoietic progenitors was significantly better than that of ECM.


CONCLUSIONS
Murine bone marrow endothelial cells support the quantitative expansion of UCB hematopoietic progenitor cells in vitro.
Wang
Jian-Hua
JH
Research Lahboratory of Blood Physiology, Central South University, Changsha, China.
Cheng
La-Mei
LM
Ma
Li-Xia
LX
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media; D042783:Endothelial Cells / Q000166:cytology*; D005312:Fetal Blood / Q000166:cytology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice
D003470:Culture Media; D016222:Fibroblast Growth Factor 2