[Serum anti-oxidative barrier in acute pancreatitis].
Serum anti-oxidative barrier is a factor determining active oxygen form suppression. The aim of this study is to investigate superoxide dismutase (SOD) and glutathione peroxidase (GP) serum level changes as a marker of its enzymatic barrier efficiency in patients with acute pancreatitis (AP) evaluated by means of Ranson score.


METHODS
Fifty patients with AP were divided onto 2 groups. Group 1 consisted of 22 patients with moderate AP, whose overall standing varied from 3 to 6 points according to Ranson. Group 2 was made of 28 patients with severe AP and 6 or more Ranson scores. Serum activities of SOD and GP were measured on 1st, 3rd 7th, and 10th day of treatment using Martin oxidation method in the former, and Rice-Evans spectrophotometry method in the latter case.


RESULTS
Blood SOD activity in Group 1 patients underwent gradual normalization; however in all time intervals it remained statistically higher than in Group 2. The activity of GP was lower than normal in both groups. In moderate AP patient group GP activity differences became statistically significant as late as on the 7th and 10th day of study. Both patient groups differed significantly regarding the GP serum activity. Serum anti-oxidative barrier activity in patients with severe AP was significantly lower than in patients with moderate form of the disease.


CONCLUSIONS
Along with patient amelioration and inflammation regression the activity of anti-oxidative serum enzymes increased. The estimation of anti-oxidative stress parameters may be useful in disease dynamics monitoring which affects treatment modality.
Modzelewski
Bogdan
B
Klinika Chirurgii Ogólnej i Gastroenterologicznej Uniwersytetu Medycznego, Lodzi. Bogdanm@ventura.pl
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019283:Pancreatitis, Acute Necrotizing / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000097:blood*
D015415:Biomarkers; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase