Inhibition of human UDP-glucose dehydrogenase expression using siRNA expression vector in breast cancer cells.
UDP-glucose dehydrogenase (UGDH) catalyzes two oxidations of UDP-glucose to yield UDP-glucuronic acid. Pathological over-production of extracellular matrix components may be linked to the availability of UDP-glucuronic acid, therefore UGDH is a potential therapeutic target. RNA interference (RNAi) has been adapted to knock down the expression of human UGDH. A UGDH siRNA plasmid was constructed using a pRNA-U6.1/Neo vector and transfected into breast cancer cells, ZR-75-1, with an efficiency of up to 50%. Western blot analysis showed that the UGDH expression was efficiently knocked down at protein levels by RNAi in ZR-75-1 cells.
Huh
Jae-Wan
JW
Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, 388-1 Poongnap-dong, 138-736, Seoul, Songpa-gu, Korea.
Choi
Myung-Min
MM
Yang
Seung-Ju
SJ
Yoon
Seung Yong
SY
Choi
Soo Young
SY
Cho
Sung-Woo
SW
D001943:Breast Neoplasms / Q000201:enzymology* / Q000235:genetics*; D045744:Cell Line, Tumor; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000235:genetics* / Q000378:metabolism*
D034741:RNA, Small Interfering; D014533:Uridine Diphosphate Glucose Dehydrogenase