Secretion of recombinant pediocin PA-1 by Bifidobacterium longum, using the signal sequence for bifidobacterial alpha-amylase.
A recombinant DNA, encoding the chimeric protein of the signal sequence for bifidobacterial alpha-amylase mature pediocin PA-1, was introduced into Bifidobacterium longum MG1. Biologically active pediocin PA-1 was successfully secreted from the strain and showed bactericidal activity against Listeria monocytogenes and the same molecular mass as native pediocin PA-1.
Moon
Gi-Seong
GS
Food Safety Research Division, Korea Food Research Institute, San 46-1, Baekhyun-Dong, Bundang-Gu, Seongnam, Gyeonggi 463-746, Korea.
Pyun
Yu-Ryang
YR
Park
Myeong Soo
MS
Ji
Geun Eog
GE
Kim
Wang June
WJ
D001430:Bacteriocins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D001644:Bifidobacterium / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008089:Listeria monocytogenes / Q000187:drug effects*; D000070017:Pediocins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism
D001430:Bacteriocins; D000070017:Pediocins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C074288:pediocin PA-1; D000516:alpha-Amylases