Influence of human ascitic fluid on the in vitro antibacterial activity of moxifloxacin.
We investigated the in vitro influence of HAF on the antibacterial activity of moxifloxacin against Escherichia coli ATCC 10798, Escherichia coli K-12, Proteus rettgeri (Sanelli), Staphylococcus aureus ATCC 25923, Staphylococcus aureus NCTC 1808 and Staphylococcus epidermidis ATCC 12228. Human ascitic fluid was obtained from 6 cirrhotic patients by paracentesis. The interaction effect was evaluated by the checkerboard technique. Our results indicate the ability of human ascitic fluid to reduce minimum inhibitory concentrations of moxifloxacin against Gram-negative bacteria, but not against Gram-positives.
Miglioli
P A
PA
Department of Pharmacology and Anaesthesiology, University of Padova, Italy. pierandrea.miglioli@unipd.it
Cappellari
G
G
Cavallaro
A
A
Cardaioli
C
C
Sossai
P
P
Fille
M
M
Allerberger
F
F
D001202:Ascitic Fluid / Q000737:chemistry / Q000382:microbiology*; D001372:Aza Compounds / Q000494:pharmacology*; D024841:Fluoroquinolones; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D000077266:Moxifloxacin; D011336:Probability; D011804:Quinolines / Q000494:pharmacology*; D012494:Sampling Studies; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
D001372:Aza Compounds; D024841:Fluoroquinolones; D011804:Quinolines; D000077266:Moxifloxacin