Biodegradation of azo dyes by genetically engineered azoreductase.
A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1.1737. The enzyme, with a molecular weight of 18.7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.
Wang
Jing
J
School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116023, China. wangjing1967@sohu.com
Yan
Bin
B
Zhou
Ji-Ti
JT
Bao
Yong-Ming
YM
Lu
Hong
H
Yuan
Xiao-Dong
XD
D001391:Azo Compounds / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000378:metabolism*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005818:Genetic Engineering; D013058:Mass Spectrometry; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D009601:Nitroreductases; D010957:Plasmids
D001391:Azo Compounds; D004396:Coloring Agents; D017931:DNA Primers; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase