[Expression of gastrin and gastrin releasing peptide in patient's with gastric cancer by using tissue chip technique].
OBJECTIVE
To study the expression of gastrin(GAS) and gastrin releasing peptide(GRP) in patients with gastric cancer and investigate the clinical significance.


METHODS
The expression of GAS and GRP in sixty patients with gastric cancer was detected by using tissue chip technique and immunohistochemical methods.


RESULTS
The positive rates of GAS and GRP were 30.0% and 11.7% respectively in 60 cases with gastric cancer. The positive rates of GAS and GRP were higher in moderately and poorly differentiated cancers than those in well differentiated cancer (P< 0.05). The positive rates of GAS and GRP were significantly higher in mucinous adenocarcinoma and signet-ring cell carcinoma than those in other types of gastric cancer (P< 0.05). The positive expression of GAS and GRP in gastric cancer was correlated with lymph node metastasis (P< 0.05).


CONCLUSIONS
Tissue chip technique is a feasible,rapid,economic and accurate approach for screening clinical tissue specimens on a large scale.
Lu
Ming-zhi
MZ
Department of Pathology, Jiangxi Provincial People's Hospital, Nanchang, China.
Liu
Yong
Y
Dai
Yan-zhi
YZ
Yuan
Cheng
C
Deng
Yu
Y
D000328:Adult; D000368:Aged; D005260:Female; D019886:Gastrin-Releasing Peptide / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D040081:Protein Array Analysis; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
D005755:Gastrins; D019886:Gastrin-Releasing Peptide