title: Evaluation of the effect of incorporation of dibutyryl cyclic adenosine monophosphate in an in situ-forming hydrogel wound dressing based on oxidized alginate and gelatin.
abstract: Cyclic adenosine monophosphate (cAMP) has long been regarded as a second messenger and a regulator of human keratinocyte proliferation. To explore more effective wound management, dibutyryl cyclic adenosine monophosphate (DBcAMP), a lipophilic analog of cAMP was incorporated into an in situ-forming hydrogel wound dressing based on periodate-oxidized alginate and gelatin. In vitro release of DBcAMP from the matrix into phosphate buffered saline was slow and increased with time. Only 50-60% of the compound was released into the medium over a period of 2 days suggestive of a sustained release into the wound bed over a period of few days. The wound-healing efficacy of the DBcAMP-incorporated dressing was evaluated on experimental full-thickness wounds in a rat model. It was found that dressing promoted wound healing leading to complete re-epithelialization of wounds within 10 days, whereas control wounds took 15 days for complete re-epithelialization. Data obtained in this study showed that the presence of DBcAMP accelerated healing and re-epithelialization of full-thickness wounds.
authors.affiliation: Division of Polymer Chemistry, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Satelmond Palace Campus, Trivandrum 695 012, Kerala, India.
authors.forename: Biji
authors.initials: B
authors.lastname: Balakrishnan
authors.forename: Mira
authors.initials: M
authors.lastname: Mohanty
authors.forename: Adelaide C
authors.initials: AC
authors.lastname: Fernandez
authors.forename: Parayanthara V
authors.initials: PV
authors.lastname: Mohanan
authors.forename: A
authors.initials: A
authors.lastname: Jayakrishnan
mesh_terms: D000464:Alginates / Q000378:metabolism*; D000818:Animals; D001672:Biocompatible Materials; D001684:Biological Dressings; D003994:Bucladesine; D004353:Drug Evaluation, Preclinical; D005780:Gelatin / Q000378:metabolism*; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D020100:Hydrogels; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020100:Hydrogels; D003994:Bucladesine; D020723:Glucuronic Acid; D005780:Gelatin