title: Structural analysis of an N-glycan with "beta1-4 bisecting branch" from human serum IgG by negative-ion MSn spectral matching and exoglycosidase digestion.
abstract: A novel N-linked oligosaccharide (N-glycan) with "beta1-4 bisecting branch (galactose beta1-4 bisecting N-acetylglucosamine)" was found in human serum IgG. Its structure was efficiently analyzed by using beta-galactosidase digestion, a MSn spectral library database, and negative-ion MS2 spectral matching. For confirmation, the novel N-glycan was synthesized by using an expected standard N-glycan (acceptor), UDP-galactose (donor), and beta1-4 galactosyltransferase. This work also demonstrates that the MSn spectral library database, in particular, negative-ion MS2 spectral matching, can efficiently reduce the number of specific, sequential exoglycosidase digestions required and is useful for rapid structural analysis of unknown glycans not in the database.
authors.affiliation: Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 001-0021, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takegawa
authors.forename: Kisaburo
authors.initials: K
authors.lastname: Deguchi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
mesh_terms: D000818:Animals; D002417:Cattle; D005690:Galactose / Q000737:chemistry / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood / Q000737:chemistry*; D007477:Ions / Q000737:chemistry*; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D013997:Time Factors
chemical_list: D007074:Immunoglobulin G; D007477:Ions; D011134:Polysaccharides; D005700:Galactosyltransferases; D006026:Glycoside Hydrolases; D005690:Galactose