Rapid identification of Candida species and other clinically important yeast species by flow cytometry.
Two rapid diagnostic assays, utilizing two different Luminex flow cytometry methods, were developed for identification of clinically important ascomycetous yeast species. Direct hybridization and allele-specific primer extension methods were both successful in establishing a DNA-based assay that can rapidly and accurately identify Candida albicans, Candida krusei, Candida parapsilosis, Candida glabrata, and Candida tropicalis as well as other clinical species. The direct hybridization assay was designed to identify a total of 19 ascomycetous yeast species, and the allele-specific primer extension assay was designed to identify a total of 34 species. Probes were validated against 438 strains representing 303 species. From culture to identification, the allele-specific primer extension method takes 8 h and the direct hybridization method takes less than 5 h to complete. These assays represent comprehensive, rapid tests that are well suited for the clinical laboratory.
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Brent T
BT
Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.
Kurtzman
Cletus P
CP
D002175:Candida / Q000145:classification* / Q000235:genetics; D002177:Candidiasis / Q000382:microbiology; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016533:Mycological Typing Techniques; D009181:Mycoses / Q000382:microbiology*; D009693:Nucleic Acid Hybridization / Q000379:methods; D013045:Species Specificity; D013997:Time Factors; D015003:Yeasts / Q000145:classification* / Q000235:genetics
D015342:DNA Probes; D004271:DNA, Fungal