title: Preparation of Tc-99m-macroaggregated albumin from recombinant human albumin for lung perfusion imaging.
abstract: Human serum albumin (HSA) extracted from pooled blood taken from human donors is used in the production of (99m)Tc-labelled macroaggregated albumin (MAA) for lung perfusion imaging. However, concerns for the safety of blood-derived products due to potential contamination by infective agents (e.g. new variant CJD), make alternative production methods necessary. Recombinant DNA technology is a promising method of albumin production avoiding problems associated with human-derived HSA. This paper presents results comparing MAA prepared from recombinant human albumin (rHA, Recombumin) (rMAA) with in-house produced HSA MAA (hMAA) and commercially available MAA (cMAA). (99m)Tc-MAA was prepared using previously published production methods by heating a mixture of albumin and stannous chloride in acetate buffer (pH 5.4) at 70 degrees C for 20 min. Parameters investigated include aggregate size, radiolabelling efficiency, radiochemical and aggregate stability at 4 degrees C and in vitro (in whole human blood) at 37 degrees C and biodistribution studies. Results showed that rMAA could be produced with similar morphology, labelling efficiency and stability to hMAA and cMAA. Our findings confirm that rHA shows significant potential as a direct replacement for HSA in commercially available MAA.
authors.affiliation: Department of Medical Physics, University Hospital, Nottingham, UK.
authors.forename: A P
authors.initials: AP
authors.lastname: Hunt
authors.forename: M
authors.initials: M
authors.lastname: Frier
authors.forename: R A
authors.initials: RA
authors.lastname: Johnson
authors.forename: S
authors.initials: S
authors.lastname: Berezenko
authors.forename: A C
authors.initials: AC
authors.lastname: Perkins
mesh_terms: D000418:Albumins / Q000737:chemistry; D000818:Animals; D004355:Drug Stability; D005260:Female; D006801:Humans; D008168:Lung / Q000000981:diagnostic imaging*; D010316:Particle Size; D011817:Rabbits; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D011994:Recombinant Proteins / Q000737:chemistry; D013668:Technetium Tc 99m Aggregated Albumin / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D000418:Albumins; D019275:Radiopharmaceuticals; D011994:Recombinant Proteins; D013668:Technetium Tc 99m Aggregated Albumin