Identification of novel genes in the memory response to Brucella infection by cDNA arrays.
This study investigated memory responses in immune mice spleen cells to brucellosis by gene expression utilizing cDNA micro arrays. Out of a total of 1176 cDNA's 21 genes were differentially regulated in three independent experiments, and generally supported a Th1 type immune response. 10 genes were validated by real time PCR, and 3 genes (CD 86, CD 40 L and CD 132) were also analyzed by Flow Cytometry for surface protein expression. We extended these findings by studying the expression of five selected genes (IRF 1, SOCS 1, IL 2 R, IRF 7, and CXCR 4) in two independent groups of Brucella immunized mice. In this study we show the potential application of utilizing gene arrays to identify and establish new correlates of protection against a cell mediated immune response.
Paranavitana
Chrysanthi M
CM
Department of Bacterial Diseases, Walter Reed Army Institute of Research, Building 503, Forest Glen, Silver Spring, MD 20910, USA. chrysanthi.paranvitana@na.amedd.army.mil
Zelazowska
Elzbieta
E
Das
Rina
R
Izadjoo
Mina
M
Jett
Marti
M
Hoover
David
D
D000818:Animals; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007156:Immunologic Memory / Q000235:genetics*; D050856:Interferon Regulatory Factor-1; D050839:Interferon Regulatory Factor-7; D053631:Interleukin Receptor Common gamma Subunit; D051379:Mice; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C507941:IL2RG protein, human; C494257:IRF1 protein, human; C494234:IRF7 protein, human; C507942:Il2rg protein, mouse; D050856:Interferon Regulatory Factor-1; D050839:Interferon Regulatory Factor-7; D053631:Interleukin Receptor Common gamma Subunit; C494258:Irf1 protein, mouse; C494235:Irf7 protein, mouse; D010750:Phosphoproteins; D019718:Receptors, CXCR4; D015375:Receptors, Interleukin-2; D012097:Repressor Proteins; C491590:Socs1 protein, mouse; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins