Electrophoretic identification and quantitation of compounds in the polyphenolic fraction of extra-virgin olive oil.
A capillary zone electrophoresis method has been carried out to determine and quantitate some compounds of the polyphenolic fraction of virgin olive oil which have never previously been determined before using capillary electrophoresis, such as elenolic acid, ligstroside aglycon, oleuropein aglycon, and (+)-pinoresinol. The compounds were identified using standards obtained by semipreparative high-performance liquid chromatography (HPLC). A detailed method optimization was performed to separate the phenolic compounds present in olive oil using a methanol-water extract of Picual extra-virgin olive oil, and different extraction systems were compared (C18-solid phase extraction (SPE), Diol-SPE, Sax-SPE and liquid-liquid extraction). The optimized parameters were 30 mM sodium tetraborate buffer (pH 9.3) at 25 kV with 8 s hydrodynamic injection, and the quantitation was carried out by the use of two reference compounds at two different wavelengths.
Gómez Caravaca
Ana María
AM
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain.
Carrasco Pancorbo
Alegría
A
Cañabate Díaz
Beatriz
B
Segura Carretero
Antonio
A
Fernández Gutiérrez
Alberto
A
D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D005663:Furans / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D057888:Iridoid Glucosides; D039823:Iridoids; D017705:Lignans / Q000032:analysis; D000069463:Olive Oil; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000032:analysis; D010938:Plant Oils / Q000737:chemistry*; D059808:Polyphenols; D011714:Pyrans / Q000032:analysis
D005419:Flavonoids; D005663:Furans; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D017705:Lignans; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D059808:Polyphenols; D011714:Pyrans; C011867:4-hydroxyphenylethanol; C002769:oleuropein; C000352:elenolic acid; C445380:ligstroside; D010626:Phenylethyl Alcohol; C103298:pinoresinol