title: The effect of hyaluronic acid on insulin secretion in HIT-T15 cells through the enhancement of gap-junctional intercellular communications.
abstract: The transplantation of bioartificial pancreas has the potential to restore endogenous insulin secretion in type I diabetes. The bioartificial pancreas is constructed in vitro from cells and a support matrix. Hyaluronic acid (HA) is an extremely ubiquitous polysaccharide of extracellular matrix in the body and plays various biological roles. It has been suggested that high molecular weight (HMW) HA increases in the function of gap-junctional intercellular communications (GJIC) and the expression of connexin-43 (Cx43). To determine whether the function of pancreatic beta-cells is affected by gap junctions after HMW HA-treatment, we exposed HIT-T15, a clonal pancreatic beta-cell line, in various concentrations of HA for 24h, and then detected the insulin secretion and content, using an insulin assay kit by ELISA technique. The cellular functions of GJIC were assayed by dye-transfer method using the dye solution of Lucifer yellow. HA-treatment resulted in the enhancement of GJIC function, the increase of insulin release and insulin content. The results obtained in this study suggest that HA-coating increases the insulin secretion of HIT-T15 cells by the enhancement of Cx43-mediated GJIC. The results give useful information on design biocompatibility of HA when is used as a biomaterial for bioartificial pancreas.
authors.affiliation: Division of Medical Devices, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Li
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nagira
authors.forename: Toshie
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D018031:Connexin 43 / Q000096:biosynthesis / Q000235:genetics; D006224:Cricetinae; D017629:Gap Junctions / Q000502:physiology*; D006820:Hyaluronic Acid / Q000502:physiology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D007546:Isoquinolines; D023822:Tissue Engineering
chemical_list: D018031:Connexin 43; D007328:Insulin; D007546:Isoquinolines; D006820:Hyaluronic Acid; C017475:lucifer yellow