Fusion and matrix protein gene sequence analysis of paramyxoviruses of type 1(PMV-1) isolated from pigeons in Slovenia.
Paramyxoviruses of type 1 (PMV-l) isolated from pigeons were genetically analyzed. A part of the fusion and the matrix protein genes were amplified and sequenced, Typical amino acid sequences associated with virulence were determined at the fusion protein cleavage site in all PMV-1 isolates. All Slovene pigeon PMV-1 strains share high amino acid sequence similarity with other pigeon strains. In the phylogenetic tree, they are clustered together with pigeon PMV-1 isolates with moderate pathogenicity. Phylogenetic analysis obtained from the fusion and the matrix protein gene alignments showed the same branching order. Viruses circulating among pigeons were found to form quite unique lineage of virulent NDV strains.
Barlic-Maganja
Darja
D
Veterinary Faculty, University of Ljubljana, Gerbiceva 60, SI-1115, Ljubljana, Slovenia. darja.barlic@vf.uni-lj.si
Krapez
Uros
U
Mankoc
Sara
S
Toplak
Ivan
I
Rojs
Olga Zorman
OZ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000821:virology; D010856:Columbidae / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D017524:Slovenia; D014760:Viral Fusion Proteins / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics; D014774:Virulence / Q000235:genetics
D004279:DNA, Viral; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins