title: [The influence of circadian gene mPeriod2 on Lewis cancer cell].
abstract: OBJECTIVE
  To investigate the role of circadian gene mPeriod2 (mPer2) on tumor proliferation and apoptosis.
  
  
  METHODS
  The eukaryotic mPer2 expression vector (pcDNA 3.1-mPer2) based on pcDNA 3.1 was transfected into the cultured Lewis tumor cell by liposome method in vivo. The expression of mPer2 in transfected Lewis tumor cell was detected by immunohistochemistry and flowcytometry. The stable transfected cell line was screened by G418 and its proliferation and apoptosis was examined by flowcytometry.
  
  
  RESULTS
  Expression of PERIOD2 protein in pcDNA 3.1-mPer2 transfected Lewis tumor cell was proved by immunohistochemistry and flowcytometry. Flowcytometry result of mPer2 expression Lewis tumor cell showed less proliferation and high rate of apoptosis.
  
  
  CONCLUSIONS
  mPer2 expression can suppress the proliferation of tumor cell and increase the apoptosis of it.
authors.forename: Yue-qi
authors.initials: YQ
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Hua
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.forename: Hua-hu
authors.initials: HH
authors.lastname: Yin
authors.forename: Yan-you
authors.initials: YY
authors.lastname: Liu
authors.forename: Chun-lei
authors.initials: CL
authors.lastname: Yang
authors.forename: Zheng-rong
authors.initials: ZR
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D018827:Carcinoma, Lewis Lung / Q000235:genetics; D018797:Cell Cycle Proteins; D002455:Cell Division; D002940:Circadian Rhythm / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D056950:Period Circadian Proteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D009687:Nuclear Proteins; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D014157:Transcription Factors