Lipid peroxidation induced by carbon tetrachloride and its inhibition by antioxidant as evaluated by an oxidative stress marker, HODE.
We have recently proposed total hydroxyoctadecadienoic acid (HODE) as a biomarker for oxidative stress in vivo. The biological samples such as plasma, urine, and tissues were first reduced and then saponified to convert the oxidation products of linoleate to HODE. In the present study, this method was applied to measure the oxidative damage induced by the administration of carbon tetrachloride to mice and also to evaluate the capacity of antioxidant to inhibit the above damage. alpha-Tocopherol transfer protein knock out (alpha-TTP-/-) mice were used to evaluate antioxidant effect in the absence of alpha-tocopherol. The intraperitoneal administration of carbon tetrachloride to mice induced the increase in HODE in liver and plasma, which was followed by an increase in plasma glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT). F2-isoprostanes, another prevailing biomarker, were also increased similarly, but their concentration was approximately two to three orders of magnitude smaller than that of HODE. The lipophilic antioxidants such as gamma-tocopherol, gamma-tocotrienol and 2,3-dihydro-5-hydroxy-4,6-di-tert-butyl-2,2-dipentylbenzofuran (BO-653) were effective in suppressing the formation of HODE.
Yoshida
Yasukazu
Y
Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka Ikeda, Osaka 563-8577, Japan. yoshida-ya@aist.go.jp
Itoh
Nanako
N
Hayakawa
Mieko
M
Piga
Rosaria
R
Cynshi
Osamu
O
Jishage
Kou-ichi
K
Niki
Etsuo
E
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001572:Benzofurans / Q000037:antagonists & inhibitors / Q000097:blood / Q000494:pharmacology; D015415:Biomarkers / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000008:administration & dosage / Q000633:toxicity*; D002352:Carrier Proteins / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D005838:Genotype; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects*; D008054:Lipid Peroxides / Q000097:blood / Q000652:urine; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018384:Oxidative Stress; D013047:Specific Pathogen-Free Organisms; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D024508:Tocotrienols / Q000037:antagonists & inhibitors / Q000097:blood / Q000494:pharmacology; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000097:blood; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000097:blood
C106512:2,3-dihydro-5-hydroxy-2,2-dipentyl-4,6-di-tert-butylbenzofuran; D000975:Antioxidants; D001572:Benzofurans; D015415:Biomarkers; D002352:Carrier Proteins; D005231:Fatty Acids, Unsaturated; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D024508:Tocotrienols; C017763:alpha-tocopherol transfer protein; C495366:hydroxyoctadecadienoic acid; D014451:Ubiquinone; D014810:Vitamin E; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D024502:alpha-Tocopherol; C003741:ubiquinol; D001205:Ascorbic Acid