title: Hepatic glutathione S-transferase activity in flounder collected from contaminated and reference sites along the Polish coast.
abstract: The aim of the present study was to examine the activity of hepatic glutathione S-transferase (GST) in flounder, Platichthys flesus, as a potential biomarker of exposure to xenobiotics present in the environment. The fish were collected along known pollution gradients and from areas regarded relatively free of anthropogenic input. GST was measured spectrophotometrically in each sampled specimen of flounder. The databases from the studies performed by two research institutes were compiled, and generalized linear models were used to analyze the dependence of the enzyme activity on the time and area of sampling and on selected biological parameters of the fish. Fish sex, sampling area, year, month, water temperature, and somatic indices (gonado-somatic index, hepato-somatic index) were significant in the model of GST activity. The three annual datasets showed that the biomarker activities exhibited in the Gulf of Gdansk were significantly different from those measured in the areas off the coast.
authors.affiliation: Department of Fishery Oceanography and Marine Ecology, Sea Fisheries Institute, ul. Kollataja 1, 81-332, Gdynia, Poland. dorota@mir.gdynia.pl
authors.forename: Dorota
authors.initials: D
authors.lastname: Napierska
authors.forename: Justyna
authors.initials: J
authors.lastname: Kopecka
authors.forename: Magdalena
authors.initials: M
authors.lastname: Podolska
authors.forename: Janusz
authors.initials: J
authors.lastname: Pempkowiak
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004784:Environmental Monitoring; D005260:Female; D005432:Flounder / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D011044:Poland; D012737:Sex Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015262:Xenobiotics / Q000633:toxicity
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; D005982:Glutathione Transferase