title: IL-10 promoter single nucleotide polymorphisms are significantly associated with resistance to leprosy.
abstract: The minor haplotype -3575A/-2849G/-2763C in IL-10 promoter has been defined as a marker of disease resistance to leprosy and its severity in Brazilian population. Our investigation of six single-nucleotide polymorphisms (SNPs) in IL-10 promoter in 282 Indian leprosy patients and 266 healthy controls by direct PCR sequencing, however, showed that the extended haplotype: -3575T/-2849G/-2763C/-1082A/-819C/-592C was associated with resistance to leprosy per se and to the development of severe form of leprosy, using either a binomial (controls vs cases, P=0.01, OR=0.58, CI=0.37-0.89) or ordinal (controls vs paucibacillary vs multibacillary, P=0.004) model. Whereas, IL-10 haplotype -3575T/-2849G/-2763C/-1082A/-819T/-592A was associated with the risk of development of severe form of leprosy (P=0.0002) in contrast to the minor risk haplotype -3575T/-2849A/-2763C in the Brazilian population. The role of IL-10 promoter SNPs in Brazilian and Indian population strongly suggests the involvement of IL-10 locus in the outcome of leprosy.
authors.affiliation: National Centre of Applied Human Genetics, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Dheeraj
authors.initials: D
authors.lastname: Malhotra
authors.forename: Katayoon
authors.initials: K
authors.lastname: Darvishi
authors.forename: Soni
authors.initials: S
authors.lastname: Sood
authors.forename: Swarkar
authors.initials: S
authors.lastname: Sharma
authors.forename: Chander
authors.initials: C
authors.lastname: Grover
authors.forename: Vineet
authors.initials: V
authors.lastname: Relhan
authors.forename: B S N
authors.initials: BS
authors.lastname: Reddy
authors.forename: R N K
authors.initials: RN
authors.lastname: Bamezai
mesh_terms: D001938:Brazil; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics* / Q000276:immunology; D007199:Indians, South American; D016753:Interleukin-10 / Q000235:genetics*; D007918:Leprosy / Q000235:genetics* / Q000276:immunology; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics
chemical_list: D005819:Genetic Markers; D016753:Interleukin-10