Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway.
We used two species of light-requiring seeds, Paulownia tomentosa, which have absolute light requirement (no germination in darkness), and Stellaria media seeds, which germinate in darkness to a certain extent because of presence of preformed active phytochrome, to obtain results strongly suggesting that gibberellic acid nitrite stimulates seed germination via its capability as a functional NO donor. Exogenous application of gibberellic acid nitrite stimulates gibberellin-insensitive Stellaria media seed germination in darkness as do a wide variety of NO donors. Pure gibberellic acid could replace the light requirement of P. tomentosa seeds, thus enabling them to germinate in darkness. Gibberellic acid nitrite did not have this effect. A stimulative effect from gibberellic acid nitrite could be detected only after exposure of these seeds to short, 10 min, pulse of red light. Taken together, these results suggest that gibberellic activity of gibberellic acid nitrite is lost after nitrosation but, regarding to the presence of -O-NO moiety in the molecule, gibberellic acid nitrite shares stimulative properties in seed germination with other compounds with NO-releasing properties.
Jovanović
Vladan
V
Agricultural Research Institute Serbia, Pesticide and Environmental Research Centre, Zemun, Serbia and Montenegro.
Giba
Zlatko
Z
Djoković
Dejan
D
Milosavljević
Slobodan
S
Grubisić
Dragoljub
D
Konjević
Radomir
R
D004305:Dose-Response Relationship, Drug; D018525:Germination / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D005875:Gibberellins / Q000494:pharmacology*; D008027:Light; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009573:Nitrites / Q000494:pharmacology*; D015538:Nitrosation; D012639:Seeds / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects
D005875:Gibberellins; D020030:Nitric Oxide Donors; D009573:Nitrites; D009569:Nitric Oxide; C007842:gibberellic acid