title: Cloning and characterization of a novel splice variant of human Rab18 gene (RAB18).
abstract: Rab GTPase proteins are a kind of small GTP-binding proteins, which functions mainly focus on regulating interacellular trafficking pathways during vesicular transport. To date, 60 distinct human RAB proteins have been identified. RAB18 gene is discovered from endothelial cells. Its function is considered as endosomes and plasma membrane recycling. Research indicates RAB18 may relate to inflammation and some kinds of tumor. Here we report a splice variant of RAB18, which is 2571 bp in length and has an open reading frame coding a predicted 235 amino-acids protein. RT-PCR shows that the cDNA has different expression pattern with RAB18 and is highly expressed in testis.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Tonghai
authors.initials: T
authors.lastname: Dou
authors.forename: Chaoneng
authors.initials: C
authors.lastname: Ji
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gu
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Ye
authors.forename: Kang
authors.initials: K
authors.lastname: Ying
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D005260:Female; D019204:GTP-Binding Proteins / Q000737:chemistry; D006801:Humans; D007249:Inflammation; D008297:Male; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D002679:Pan troglodytes; D011485:Protein Binding; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution; D020691:rab GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; C484168:RAB18 protein, human; D019204:GTP-Binding Proteins; D020691:rab GTP-Binding Proteins