title: Capillary electrophoresis analysis of mangiferin extracted from Mangifera indica L. bark and Mangifera persiciformis C.Y. Wu et T.L. Ming leaves.
abstract: The flavonoid compound mangiferin is found in the leaves, stem bark, fruit peels and root of Mangifera indica L. and in many other herbal species with many potential pharmacological properties. We have established an analytical method of mangiferin extracted from M. indica L. bark and Mangifera persiciformis C.Y. Wu et T.L. Ming leaves utilizing CZE. An electrolytic buffer containing 0.05 M borate buffer, pH 7.4 with methanol (1:0.3, v/v) was deemed suitable for mangiferin analysis. An ideal mangiferin electropherogram with a migration time at approximately 10.50 min was obtained. Repeatability tests showed that the R.S.D.s for both intra- and inter-day migration time and peak area for all manigferin sources tested were less than 4%. The linearity range of this method was 5-1000 microg/ml. The detection limit of this method was 1.5 microg/ml. Quantitative analysis of mangiferin was also performed with this method. The accuracy of quantitation at 10, 500 and 1000 microg/ml of control mangiferin were 99.00, 99.38 and 99.14%, respectively (n=10). The repeatability of quantitation (R.S.D.) was below 3%. Our results demonstrated that CZE is a simple and reliable method in mangiferin analysis and more studies are needed to detect other mangiferin resources, such as clinical biological samples, in pharmacology and pharmacokinetic studies.
authors.affiliation: Department of Experimental Center, Guangxi Nationalities Hospital, Nanning, Guangxi Province, China.
authors.forename: Chaozan
authors.initials: C
authors.lastname: Nong
authors.forename: Weisheng
authors.initials: W
authors.lastname: He
authors.forename: Debbi
authors.initials: D
authors.lastname: Fleming
authors.forename: Lili
authors.initials: L
authors.lastname: Pan
authors.forename: Huayi
authors.initials: H
authors.lastname: Huang
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D031022:Mangifera / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044004:Xanthones / Q000032:analysis*
chemical_list: D044004:Xanthones; C013592:mangiferin