[Apoptosis of neutrophil in peripheral blood in experimental sepsis].
OBJECTIVE
To evaluate the changes in apoptosis of neutrophil in peripheral blood in sepsis in rats.


METHODS
The rat sepsis model was reproduced by cecum ligation and puncture (CLP). One hundred and forty-four rats were randomly divided into normal control group, sham operation group and 2, 6, 12, 24, 48, 72 hours after CLP groups. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to identify neutrophil apoptosis.


RESULTS
In early period after CLP, neutrophil apoptosis in peripheral blood was limited with a positive rate of less than 5.00%. The positive rate rose to (48.33+/-12.53)% at 48 hours, and it began to lower, approaching the normal level at 72 hours after CLP.


CONCLUSIONS
Death is the main pathway of loss of neutrophils which are produced in the acute phase of sepsis, and apoptosis is the main pathway of loss of neutrophil in the later phase of sepsis.
Xi
Jian-cheng
JC
Department of Orthopaedics, the 309 th Hospital of PLA, Beijing 100091, China.
Zhou
Bao-tong
BT
Jiang
Li-xian
LX
Jiang
Xiao-guo
XG
Sun
Tong-zhu
TZ
Yang
Yi
Y
D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D008297:Male; D009504:Neutrophils / Q000473:pathology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000097:blood*