title: Devosia limi sp. nov., isolated from a nitrifying inoculum.
abstract: A Gram-negative, rod-shaped, non-spore-forming bacteria was isolated from a nitrifying inoculum. On the basis of 16S rRNA gene sequence similarity, this strain, designated LMG 22951(T), was shown to belong to the 'Alphaproteobacteria' and to be related to Devosia neptuniae (97.4%) and Devosia riboflavina (97.0%). The results of DNA-DNA hybridization, analysis of fatty acid composition, SDS-PAGE, physiological and biochemical tests allowed genotypic and phenotypic differentiation of LMG 22951(T) from the two recognized Devosia species. LMG 22951(T) therefore represents a novel species within this genus, for which the name Devosia limi is proposed. The type strain is LMG 22951(T) (=DSM 17137(T)).
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Bram
authors.initials: B
authors.lastname: Vanparys
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Kim
authors.initials: K
authors.lastname: Heylen
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Liesbeth
authors.initials: L
authors.lastname: Lebbe
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: De Vos
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009573:Nitrites / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009573:Nitrites; D012336:RNA, Ribosomal, 16S