Effect of transgenic expression of porcine interleukin-6 gene and CpG sequences on immune responses of newborn piglets inoculated with Pseudorabies attenuated vaccine.
Porcine interleukin-6 gene and CpG sequences were used as immunoadjuvants to enhance the immune responses of newborn piglets to Pseudorabies attenuated vaccine (PAV). The titer of specific antibodies to PAV, the proliferation of lymphocytes and induced IL-2 activities were all examined to identify the immune response of the piglets. The results showed that the immune responses with CpG ODN and porcine interleukin-6 gene were significantly stronger than routine immunities. The data suggests that porcine IL-6 and CpG motifs could be employed as effective immunoadjuvants to raise the humoral and cellular responses of newborn piglets to Pseudorabies attenuated vaccine.
Ling-Hua
Zhang
Z
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510640, China. lhzhang73212000@yahoo.com.cn
Xing-Shan
Tian
T
Yong
Guo
G
Feng-Zhen
Zhou
Z
Min-Jie
Meng
M
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D049109:Cell Proliferation; D018899:CpG Islands / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D011557:Pseudorabies / Q000517:prevention & control*; D022421:Pseudorabies Vaccines / Q000276:immunology* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology*; D014613:Vaccines, Attenuated / Q000276:immunology / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007376:Interleukin-2; D015850:Interleukin-6; D022421:Pseudorabies Vaccines; D011994:Recombinant Proteins; D014613:Vaccines, Attenuated