title: High-performance liquid chromatographic method for simultaneous quantification of eight major biologically active ingredients in 'Da-Chai-Hu-Tang' preparation.
abstract: A simple, sensitive and specific high-performance liquid chromatographic method has been developed for the first time to simultaneously determine the eight major biologically active ingredients, namely paeoniflorin, naringin, sennoside A, baicalin, baicalein, saikosaponin a, rhein and emodin of the Chinese herbal formula Da-Chai-Hu-Tang. The contents of these marker substances in Da-Chai-Hu-Tang extract could be easily determined within 85 min. The assay was reproducible and accurate with overall intra-day variations and accuracy of less than 2% and more than 97.9%, respectively. Results indicate that the developed HPLC assay can be successfully utilized as a quality control method for simultaneous determination of eight representative substances in Da-Chai-Hu-Tang.
authors.affiliation: Department of Chemistry, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chia-Ying
authors.initials: CY
authors.lastname: Li
authors.forename: Chiung-Hui
authors.initials: CH
authors.lastname: Chiu
authors.forename: Ho-Shin
authors.initials: HS
authors.lastname: Huang
authors.forename: Chung-Hua
authors.initials: CH
authors.lastname: Lin
authors.forename: Tian-Shung
authors.initials: TS
authors.lastname: Wu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004365:Drugs, Chinese Herbal; C051121:daisaikoto