A beta-(1-->3)-arabinopyranosyltransferase that transfers a single arabinopyranose onto arabino-oligosaccharides in mung bean (Vigna radiate) hypocotyls.
Arabinopyranosyltransferase (ArapT) activity that results in the transfer of a single arabinopyranose (Arap) residue from UDP-beta-L-arabinopyranose (UDP-Arap) to exogenous (1-->5)-linked alpha-L-arabino-oligosaccharides labeled with 2-aminobenzamide (2-AB) at their reducing ends was identified in a particulate preparation obtained from 3-day-old mung bean (Vigna radiate L. Wilezek) hypocotyls. The transferred Ara residue was shown to be beta-(1-->3)-linked to O-3 of the non-reducing terminal Araf residues of the oligosaccharide using nuclear magnetic resonance spectroscopy together with glycosyl composition and glycosyl linkage composition analyses. The 2AB-labeled arabino-octasaccharide was the most effective acceptor substrate analyzed, although arabino-oligosaccharides with a degree of polymerization between 4 and 7 were also acceptor substrates. Maximum ArapT activity was obtained at pH 6.5-7.0, and 20 degrees C in the presence of 25 mM Mn(2+) and 0.5% Triton X-100.
Ishii
Tadashi
T
Forestry and Forest Products Research Institute, Tsukuba Norin Kenkyu, Danchi-Nai, Ibaraki, Japan. tishii@ffpri.affrc.go.jp
Konishi
Teruko
T
Ito
Yuki
Y
Ono
Hiroshi
H
Ohnishi-Kameyama
Mayumi
M
Maeda
Ikuko
I
D001089:Arabinose / Q000737:chemistry / Q000378:metabolism; D002236:Carbohydrate Conformation; D007887:Fabaceae / Q000737:chemistry* / Q000378:metabolism; D018546:Hypocotyl / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008861:Microsomes / Q000378:metabolism; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D010430:Pentosyltransferases / Q000737:chemistry* / Q000378:metabolism*; D062367:ortho-Aminobenzoates / Q000737:chemistry
D009844:Oligosaccharides; D062367:ortho-Aminobenzoates; D001089:Arabinose; D010430:Pentosyltransferases; C506429:beta-(1-3)-arabinopyranosyltransferase, Vigna radiata; C000219:anthranilamide