Gene analysis of Cryptosporidium parvum HNJ-1 strain isolated in Japan.
We analyzed genetically Cryptosporidium parvum HNJ-1 strain, which is the Japanese reference strain isolated from human in Japan. DNA sequences of genes for thrombospondin-related adhesive protein of Cryptosporidium-1 and Cryptosporidium-2 (TRAP-C1, TRAP-C2), heat shock protein 70 (HSP70), oocyst wall protein (COWP), beta-tubulin, alpha-tubulin, polythreonine-region (Poly-T), elongation factor 1 alpha (EF-1 alpha), and 18S rRNA of this strain were determined. They showed high rate of homology to published sequences of genotype 2 strains, which were considered to be infective to both humans and animals. However, HNJ-1 had synonymous and non-synonymous substitutions in the nucleotide sequence of TRAP-C1 and beta-tubulin among HNJ-1 and published sequences of genotype 2 strains. These results implied that HNJ-1 strain was an unique subpopulation of genotype 2 strain of C. parvum.
Satoh
Masaaki
M
Laboratory of Animal Health and Management, Graduate School of Tohoku University, Naruko, 989-6711 Miyagi, Japan.
Kimata
Isao
I
Iseki
Motohiro
M
Nakai
Yutaka
Y
D000328:Adult; D019943:Amino Acid Substitution; D000818:Animals; D003457:Cryptosporidiosis / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D005260:Female; D017125:Genes, Protozoan; D006801:Humans; D007564:Japan; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D020539:Sequence Analysis, Protein; D013045:Species Specificity; D014404:Tubulin / Q000235:genetics
D020648:Peptide Elongation Factor 1; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S; D014404:Tubulin; C069428:thrombospondin-related adhesive protein, protozoan