Enterococcus canintestini sp. nov., from faecal samples of healthy dogs.
The taxonomic position of strain LMG 13590(T), originally isolated from dog faeces and classified as Enterococcus dispar in the BCCM/LMG Bacteria Catalogue, was reinvestigated. This strain and 12 recent isolates from faecal samples of healthy dogs occupied a clearly separate position when investigated with multilocus sequence analysis (MLSA) of the genes encoding the alpha subunit of ATP synthase (atpA), RNA polymerase alpha subunit (rpoA) and phenylalanyl-tRNA synthase alpha subunit (pheS). The 16S rRNA gene sequence of one representative strain showed highest similarities of 98-99% with E. dispar LMG 13521(T), Enterococcus canis LMG 12316(T) and Enterococcus asini LMG 18727(T). A further polyphasic taxonomic study based on whole-cell protein fingerprinting, DNA-DNA hybridization and biochemical features demonstrated that the 13 enterococcal dog faecal strains represent a single, novel Enterococcus species for which the name Enterococcus canintestini sp. nov. is proposed. The type strain is LMG 13590(T) (=CCM 7285(T)).
Naser
Sabri M
SM
BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.|Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
Vancanneyt
Marc
M
BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
De Graef
Evelyne
E
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Devriese
Luc A
LA
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Snauwaert
Cindy
C
BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
Lefebvre
Karen
K
BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
Hoste
Bart
B
BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
Švec
Pavel
P
Czech Collection of Microorganisms, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
Decostere
Annemie
A
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Haesebrouck
Freddy
F
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Swings
Jean
J
BCCM/LMGTM Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.|Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium.
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D004285:Dogs / Q000382:microbiology*; D016983:Enterococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S