title: Sulfato/thiosulfato reducing bacteria characterization by FT-IR spectroscopy: a new approach to biocorrosion control.
abstract: Sulfato and Thiosulfato Reducing Bacteria (SRB, TRB) can induce corrosion process on steel immersed in seawater. This phenomenon, called biocorrosion, costs approximatively 5 billion euros in France each year. We provide the first evidence that Fourier Transformed InfraRed (FTIR) spectroscopy is a competitive technique to evaluate the sulfurogen flora involved in biocorrosion in comparison with time consuming classical identification methods or PCR analyses. A great discrimination was obtained between SRB, TRB and some contamination bacteria known to be present in seawater and seem to be able to reduce sulfate under particular conditions. Moreover, this preliminary study demonstrates that FTIR spectroscopic and genotypic results present a good correlation (these results are confirmed by other data obtained before or later, data not shown here). The advantages gained by FTIR spectroscopy are to give information on strain phenotype and bacterial metabolism which are of great importance in corrosion processes.
authors.affiliation: Corrodys, Equipe de Recherche en Physico-Chimie et Biotechnologie, EA 1852, IUT Caen, Université Caen Basse Normandie, 14032 Caen Cedex, France.
authors.forename: Celine
authors.initials: C
authors.lastname: Rubio
authors.forename: Christelle
authors.initials: C
authors.lastname: Ott
authors.forename: Caroline
authors.initials: C
authors.lastname: Amiel
authors.forename: Isabelle
authors.initials: I
authors.lastname: Dupont-Moral
authors.forename: Josette
authors.initials: J
authors.lastname: Travert
authors.forename: Laurence
authors.initials: L
authors.lastname: Mariey
mesh_terms: D018441:Biofilms; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D016999:Sulfur-Reducing Bacteria / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S