title: [Determination of cholesteryl palmitate in Oviductus Ranae by HPLC].
abstract: OBJECTIVE
  To develop a new method for the determination of cholesteryl palmitate in Oviductus Ranae.
  
  
  METHODS
  A HPLC method was set up, using Zorbax Silica column and cyclohexane-diethyl ether (40:1) as mobile phase with a flow rate of 1.0 mL x min(-1), and the UV detection wavelength was 203 nm.
  
  
  RESULTS
  The calibration curve was linear over the range of 0.60-8.92 microg (r = 0.9997), the average recovery of the method was 98.4%. RSD 1.8% (n = 6).
  
  
  CONCLUSIONS
  The results showed that method was reliable and accurate.
authors.affiliation: Jilin University, Changchun 130021, China.
authors.forename: Yong-sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Da-cheng
authors.initials: DC
authors.lastname: Jiang
authors.forename: Qin
authors.initials: Q
authors.lastname: Meng
authors.forename: En-si
authors.initials: ES
authors.lastname: Wang
mesh_terms: D000818:Animals; D002788:Cholesterol Esters / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D008419:Materia Medica / Q000032:analysis* / Q000737:chemistry; D010057:Oviducts / Q000737:chemistry*; D011786:Quality Control; D011896:Rana temporaria
chemical_list: D002788:Cholesterol Esters; D008419:Materia Medica; C539466:Oviductus Ranae; C026651:cholesteryl palmitate