title: Molecular basis for the substrate selectivity of bicyclic and monocyclic extradiol dioxygenases.
abstract: Extradiol dioxygenases play a key role in determining the specificities of the microbial aromatic catabolic pathways in which they occur. To identify the structural determinants of specificity in this class of enzymes, variants of 2,3-dihydroxybiphenyl (DHB) 1,2-dioxygenase (DHBD) were investigated. Structural data of the DHBD/DHB complex informed the design of seven variants at four positions: V148W, V148L, M175W, A200I, A200W, P280W, and V148L/A200I. All variants had reduced specificity for DHB. In addition, the V148W, V148L, A200I, and V148L/A200I variants had increased specificity for catechol. Indeed, the V148W variant had a higher apparent specificity for 3-Me catechol than for DHB, although the substitution reduced the kcat for all tested substrates as well as the rate constant of suicide inactivation of the enzyme. These results are consistent with available structural data which suggest that the larger residue at position 148 may partially occlude O2 binding. The results further indicate that in addition to defining substrate specificity, the binding pocket orientates the bound catechol to minimize oxidative inactivation of the enzyme during catalysis.
authors.affiliation: Department of Microbiology, University of British Columbia, Vancouver, BC, Canada V6T 1Z3.
authors.forename: Frédéric H
authors.initials: FH
authors.lastname: Vaillancourt
authors.forename: Pascal D
authors.initials: PD
authors.lastname: Fortin
authors.forename: Geneviève
authors.initials: G
authors.lastname: Labbé
authors.forename: Nathalie M
authors.initials: NM
authors.lastname: Drouin
authors.forename: Zamil
authors.initials: Z
authors.lastname: Karim
authors.forename: Nathalie Y R
authors.initials: NY
authors.lastname: Agar
authors.forename: Lindsay D
authors.initials: LD
authors.lastname: Eltis
mesh_terms: D001665:Binding Sites / Q000235:genetics; D001713:Biphenyl Compounds / Q000378:metabolism; D020134:Catalytic Domain / Q000235:genetics; D002396:Catechols / Q000378:metabolism; D049308:Dioxygenases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D007501:Iron / Q000737:chemistry / Q000302:isolation & purification; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D001713:Biphenyl Compounds; D002396:Catechols; C454813:2,3-dihydroxybiphenyl; D007501:Iron; D010105:Oxygenases; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase; C439587:extradiol dioxygenase; D010100:Oxygen