title: Heat stress promotes mitochondrial instability and oxidative responses in yeast deficient in thiazole biosynthesis.
abstract: The Thi4 protein from Saccharomyces cerevisiae plays a pivotal role in the biosynthesis of thiazole, a precursor of thiamine (vitamin B1). In addition, the thi4-disrupted strain has shown increased frequencies of mitochondrial mutants (petite colonies) upon treatment with DNA damaging agents. In this work, we show that the thi4 strain presents significant induction of petites and reduced oxygen consumption when grown at 37 degrees C, a condition that induces high levels of reactive oxygen species in yeast. Oxidative stress parameters were thus measured in thi4 cells. The activities of superoxide dismutase and phospholipid hydroperoxide glutathione peroxidase were significantly increased when these mutants were grown at 37 degrees C compared to the wild-type strain (W303). The levels of carbonyl protein groups were also significantly higher for the thi4 strain than for W303. Still, significant reductions in protein thiols and reduced glutathione were observed for the mutated strain. Therefore, the Thi4 protein appears to play an important protective role during heat stress in yeast cells, a feature probably related to the mitochondrial instability and altered oxidative status observed in thi4 mutants.
authors.affiliation: Department of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, Cidade Universitária, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: Medina-Silva
authors.forename: Marcelo P
authors.initials: MP
authors.lastname: Barros
authors.forename: Rodrigo S
authors.initials: RS
authors.lastname: Galhardo
authors.forename: Luis E S
authors.initials: LE
authors.lastname: Netto
authors.forename: Pio
authors.initials: P
authors.lastname: Colepicolo
authors.forename: Carlos F M
authors.initials: CF
authors.lastname: Menck
mesh_terms: D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D008928:Mitochondria / Q000502:physiology*; D009154:Mutation; D018384:Oxidative Stress / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D013438:Sulfhydryl Compounds; C089358:THI4 protein, S cerevisiae; D013844:Thiazoles; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D010100:Oxygen