title: Development of an integrated microfluidic platform for dynamic oxygen sensing and delivery in a flowing medium.
abstract: This paper describes a platform for real-time sensing of dissolved oxygen in a flowing microfluidic environment using an oxygen-sensitive luminescent dye (platinum octaethylporphyrin ketone) integrated into a micro-oxygenator device. Using a phase-based detection method, the luminescent decay lifetime of the dye was consistent with the linear Stern-Volmer relationship using both gaseous and aqueous samples. Maximum sensor resolution varied between 120-780 ppb across a range of dissolved oxygen (DO) concentrations ranging from 0-42.5 ppm. The sensor was subsequently used to determine the convective mass-transfer characteristics of a multi-layer polydimethylsiloxane (PDMS) microfluidic oxygenator. The membrane-based oxygenator showed excellent agreement with an analytical convection model, and the integrated oxygen sensor was accurate across a wide range of tested flow rates (0.05-5 mL min(-1)). The device is unique for its ease of fabrication and highly flexible configuration, as well as the novel incorporation of oxygen delivery and detection in a single micro-device. Potential applications include tissue engineering, cell culturing, and miniaturized bio-assays that require the delivery and/or detection of precise quantities of oxygen within a microfluidic construct.
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Vollmer
authors.forename: Ronald F
authors.initials: RF
authors.lastname: Probstein
authors.forename: Richard
authors.initials: R
authors.lastname: Gilbert
authors.forename: Todd
authors.initials: T
authors.lastname: Thorsen
mesh_terms: D001681:Biological Assay; D015374:Biosensing Techniques; D002138:Calibration; D002196:Capillaries; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004563:Electrochemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D044085:Microfluidics; D008904:Miniaturization; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D012828:Silicones / Q000737:chemistry; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods
chemical_list: D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon; D010100:Oxygen