Overexpression of SOX9 in mouse embryonic stem cells directs the immediate chondrogenic commitment.
Mouse embryonic stem (mES) cells are capable of undergoing chondrogenesis in vitro. To enhance this process, the human SOX9 (hSOX9) cDNA was delivered into mES cells and the clones overexpressing hSOX9 (denoted as mES-hSOX9 cells) were verified by Western blot analysis. The transcripts of collagen IIA (a juvenile form), aggrecan and Pax1 were expressed in mES-hSOX9 cells grown on feeder layers, suggesting the immediate effect of exogenous SOX9 on chondrogenesis. However, SOX9 overexpression did not affect the cell cycle distribution in undifferentiated mES cells. Upon differentiation, collagen IIB (an adult form) was detected in day 3 immature embryoid bodies. In addition, the overexpression of exogenous SOX9 significantly induced transcriptional activity driven by SOX9 binding site. Taken together, we for the first time demonstrated that constitutive overexpression of exogenous SOX9 in undifferentiated mES cells might have dual potentials to induce both chondrogenic commitment and growth capacity in the undifferentiated status.
Kim
Jae-Hwan
JH
Cell and Gene Therapy Research Institute, Pochon CHA University, Seoul 135-907, Korea.
Do
Hyun-Jin
HJ
Yang
Heung-Mo
HM
Oh
Jong-Hyun
JH
Choi
Seong-Jun
SJ
Kim
Dong-Ku
DK
Cha
Kwang-Yul
KY
Chung
Hyung-Min
HM
D053674:Aggrecans; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D020219:Chondrogenesis; D024043:Collagen Type II / Q000235:genetics; D004622:Embryo, Mammalian / Q000166:cytology*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D006609:High Mobility Group Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics; D051379:Mice; D051761:Paired Box Transcription Factors / Q000235:genetics; D011509:Proteoglycans / Q000235:genetics; D055758:SOX9 Transcription Factor; D013234:Stem Cells / Q000378:metabolism* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
C508320:Acan protein, mouse; D053674:Aggrecans; D024043:Collagen Type II; D016326:Extracellular Matrix Proteins; D005819:Genetic Markers; D006609:High Mobility Group Proteins; D037181:Lectins, C-Type; D051761:Paired Box Transcription Factors; D011509:Proteoglycans; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C527933:Sox9 protein, mouse; D014157:Transcription Factors; C070835:PAX1 transcription factor