title: Direct application of the INNO-LiPA Rif.TB line-probe assay for rapid identification of Mycobacterium tuberculosis complex strains and detection of rifampin resistance in 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis.
abstract: The INNO-LiPA Rif.TB assay for the identification of Mycobacterium tuberculosis complex strains and the detection of rifampin (RIF) resistance has been evaluated with 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis (MDR-TB). The sensitivity when compared to conventional identification/culture methods was 82.2%, and the specificity was 66.7%; the sensitivity and specificity were 100.0% and 96.9%, respectively, for the detection of RIF resistance. This assay has the potential to provide rapid information that is essential for the effective management of MDR-TB.
authors.affiliation: Unidade de Micobacterias do Instituto de Higiene e Medicina Tropical, Lisboa, Portugal.
authors.forename: Miguel
authors.initials: M
authors.lastname: Viveiros
authors.forename: Clara
authors.initials: C
authors.lastname: Leandro
authors.forename: Liliana
authors.initials: L
authors.lastname: Rodrigues
authors.forename: Josefina
authors.initials: J
authors.lastname: Almeida
authors.forename: Rosário
authors.initials: R
authors.lastname: Bettencourt
authors.forename: Isabel
authors.initials: I
authors.lastname: Couto
authors.forename: Lurdes
authors.initials: L
authors.lastname: Carrilho
authors.forename: José
authors.initials: J
authors.lastname: Diogo
authors.forename: Ana
authors.initials: A
authors.lastname: Fonseca
authors.forename: Luís
authors.initials: L
authors.lastname: Lito
authors.forename: João
authors.initials: J
authors.lastname: Lopes
authors.forename: Teresa
authors.initials: T
authors.lastname: Pacheco
authors.forename: Mariana
authors.initials: M
authors.lastname: Pessanha
authors.forename: Judite
authors.initials: J
authors.lastname: Quirim
authors.forename: Luísa
authors.initials: L
authors.lastname: Sancho
authors.forename: Max
authors.initials: M
authors.lastname: Salfinger
authors.forename: Leonard
authors.initials: L
authors.lastname: Amaral
mesh_terms: D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D012321:DNA-Directed RNA Polymerases; D024881:Drug Resistance, Bacterial; D006801:Humans; D015994:Incidence; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012293:Rifampin / Q000494:pharmacology*; D013183:Sputum / Q000382:microbiology*; D013997:Time Factors; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000382:microbiology
chemical_list: D000904:Antibiotics, Antitubercular; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin