Immunogenicity of combination DNA vaccines for Rift Valley fever virus, tick-borne encephalitis virus, Hantaan virus, and Crimean Congo hemorrhagic fever virus.
DNA vaccines for Rift Valley fever virus (RVFV), Crimean Congo hemorrhagic fever virus (CCHFV), tick-borne encephalitis virus (TBEV), and Hantaan virus (HTNV), were tested in mice alone or in various combinations. The bunyavirus vaccines (RVFV, CCHFV, and HTNV) expressed Gn and Gc genes, and the flavivirus vaccine (TBEV) expressed the preM and E genes. All vaccines were delivered by gene gun. The TBEV DNA vaccine and the RVFV DNA vaccine elicited similar levels of antibodies and protected mice from challenge when delivered alone or in combination with other DNAs. Although in general, the HTNV and CCHFV DNA vaccines were not very immunogenic in mice, there were no major differences in performance when given alone or in combination with the other vaccines.
Spik
Kristin
K
Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA.
Shurtleff
Amy
A
McElroy
Anita K
AK
Guttieri
Mary C
MC
Hooper
Jay W
JW
SchmalJohn
Connie
C
D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D017931:DNA Primers; D004669:Encephalitis Viruses, Tick-Borne / Q000254:growth & development / Q000276:immunology*; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000254:growth & development / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests; D012296:Rift Valley fever virus / Q000254:growth & development / Q000276:immunology*; D017778:Vaccines, Combined / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*; D010948:Viral Plaque Assay
D017931:DNA Primers; D017778:Vaccines, Combined; D019444:Vaccines, DNA