title: Heat shock protein 32 in human peripheral blood mononuclear cells: effect of aging and inflammation.
abstract: The purpose of the present study was to assess the influence of age and acute infection on the production of Hsp32 in human peripheral blood cells, using flow cytometry. Thirty-five controls and 31 patients with acute infection participated. We found that the age and inflammatory status correlated positively with Hsp32 levels in both heat shocked (HS) and non-HS monocytes and lymphocytes. In addition, the HS response of Hsp32 was different in these peripheral blood cells; whereas HS exerted an up-regulation in the levels of Hsp32 in monocytes, a significant decrease in Hsp32 levels was noticed for lymphocytes. We found significant relationships between circulating C-reactive protein as well as interleukin-6 and the levels of Hsp32 in cells. We conclude that Hsp32 is up-regulated in the elderly as well as in individuals with inflammation, and that the HS response of Hsp32 is different in monocytes as compared to lymphocytes.
authors.affiliation: Geriatric Unit, Academic Hospital, Vrije Universiteit Brussel, Belgium.
authors.forename: Rose
authors.initials: R
authors.lastname: Njemini
authors.forename: Margareta
authors.initials: M
authors.lastname: Lambert
authors.forename: Christian
authors.initials: C
authors.lastname: Demanet
authors.forename: Tony
authors.initials: T
authors.lastname: Mets
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D002097:C-Reactive Protein / Q000032:analysis; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D051547:Heme Oxygenase-1 / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide; D007249:Inflammation / Q000097:blood / Q000378:metabolism*; D015850:Interleukin-6 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D016877:Oxidants; D018384:Oxidative Stress; D013696:Temperature
chemical_list: D006360:Heat-Shock Proteins; D015850:Interleukin-6; D016877:Oxidants; D002097:C-Reactive Protein; D006861:Hydrogen Peroxide; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1