title: Wound healing of laparoscopic esophageal myotomy with or without an added gastric patch.
abstract: BACKGROUND
  The purpose of this research is to compare the wound healing of the laparoscopic esophagomyotomy with and without a gastric patch.
  
  
  METHODS
  Twelve male pigs were distributed into two groups of six animals. Esophagomyotomy was performed in group A. A gastric patch was associated to the myotomy in group B. On the 21st postoperative day, lumen molding was accomplished to determine the index of stenosis (IS) at the area of myotomy (AM). Macroscopic and microscopic aspects of wound healing were also studied at AM. Three microscopic morphologic patterns were defined for morphometric evaluation: leukocytes (constituted by polymorphonuclear and mononuclear cells), new endothelial cells, and collagen fibers.
  
  
  RESULTS
  There was a longer operative duration in group B (93.6 min) than in group A (45 min). At AM, IS was negative (lumen increased) and equivalent in both groups: -11.1% in group A and -12.7% in group B. Mesotelial epithelium covering RM was observed in group A. Inflammatory reaction was greater in group B in comparison with group A (leuCocytes: 22 cells versus 8.6; fibrosis: 25.5 fibers versus 15.6; granulation tissue: 18.7 vessels versus 9.7).
  
  
  CONCLUSIONS
  Esophagomyotomy followed by gastric patch does not heal adequately and is worsened by the presence of foreign body granulomas around stitches. Myotomy without gastric patch is faster and causes lower inflammation. Myotomy alone or with gastric patch does not lead to esophageal stenosis at RM and does not lead to restoration of the esophageal musculature continuity.
authors.affiliation: Division of Experimental Surgery, Department of Surgery, Federal University of São Paulo, R. Botucatu, 740-Prédio da Cirurgia Experimental-CEP, São Paulo, 04023-900, Brazil. jozevedo.dcir@epm.br
authors.forename: J L M C
authors.initials: JL
authors.lastname: Azevedo
authors.forename: F O
authors.initials: FO
authors.lastname: Kozu
authors.forename: O
authors.initials: O
authors.lastname: Azevedo
authors.forename: C E P
authors.initials: CE
authors.lastname: Silva
authors.forename: A A
authors.initials: AA
authors.lastname: Sorbello
authors.forename: M d J
authors.initials: Md
authors.lastname: Simões
authors.forename: A
authors.initials: A
authors.lastname: Delorenzo
authors.forename: R C
authors.initials: RC
authors.lastname: Pasqualin
authors.forename: G S
authors.initials: GS
authors.lastname: Aguiar
authors.forename: F J C
authors.initials: FJ
authors.lastname: Menezes
mesh_terms: D000818:Animals; D013505:Digestive System Surgical Procedures / Q000379:methods; D004947:Esophagus / Q000473:pathology / Q000601:surgery*; D010535:Laparoscopy / Q000379:methods*; D008297:Male; D009130:Muscle, Smooth / Q000473:pathology / Q000601:surgery*; D013270:Stomach / Q000637:transplantation*; D013552:Swine; D014945:Wound Healing