Multi-residue liquid chromatography/tandem mass spectrometric analysis of beta-agonists in urine using molecular imprinted polymers.
Ion suppression, a matrix effect that affects quantitative mass spectrometry, is one of the main problems encountered in liquid chromatography/tandem mass spectrometry. Two different clean-up steps for the multi-residue analysis of beta-agonists in urine were evaluated with respect to minimisation of ion suppression, namely, a mixed-phase solid phase extraction (SPE) column, i.e., clean screen Dau (CSD), and a molecular imprinted polymer (MIP) SPE column. Ion suppression experiments revealed that CSD sample clean-up can lead to false negative results for some beta-agonists, and that clean-up using MIP columns is more selective for beta-agonists than the use of CSD columns.
Van Hoof
Nathalie
N
Laboratory of Chemical Analysis, Ghent University, Department of Veterinary Public Health and Food Safety, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Courtheyn
Dirk
D
Antignac
Jean-Philippe
JP
Van de Wiele
Mieke
M
Poelmans
Sofie
S
Noppe
Herlinde
H
De Brabander
Hubert
H
D000318:Adrenergic beta-Agonists / Q000652:urine*; D016477:Artifacts; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005188:False Negative Reactions; D011108:Polymers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013499:Surface Properties
D000318:Adrenergic beta-Agonists; D011108:Polymers