title: Systems analysis of a quorum sensing network: design constraints imposed by the functional requirements, network topology and kinetic constants.
abstract: Understanding the relationship between the structural organization of intracellular decision networks and the observable phenotypes they control is one of the exigent problems of modern systems biology. Here we perform a systems analysis of a prototypic quorum sensing network whose operation allows bacterial populations to activate certain patterns of gene expression cooperatively. We apply structural perturbations to the model and analyze the resulting changes in the network behavior with the aim to identify the contribution of individual network elements to the functional fitness of the whole network. Specifically, we demonstrate the importance of the dimerization of the transcription factor and the presence of the auxiliary positive feedback loop on the switch-like behavior of the network and the stability of its "on" and "off" states under the influence of molecular noise.
authors.affiliation: Systems Biology Group, Bioinformatics Institute, 30 Biopolis Str., Singapore 138671, Singapore. andrewg@bii-sg.org
authors.forename: A B
authors.initials: AB
authors.lastname: Goryachev
authors.forename: D J
authors.initials: DJ
authors.lastname: Toh
authors.forename: T
authors.initials: T
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D003198:Computer Simulation; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D007700:Kinetics; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D015398:Signal Transduction / Q000502:physiology*; D049490:Systems Biology / Q000379:methods; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D015534:Trans-Activators; D014157:Transcription Factors