The NII2 gene of Hansenula polymorpha is involved in nitrite assimilation.
To establish a basis for genetic and molecular studies of nitrite assimilation in the methylotrophic yeast Hansenula polymorpha, we isolated and characterised six nitrite-negative mutants still capable of growing on nitrate. Gene isolation work yielded the NII2 gene, encoding a membrane protein homologous to the Saccharomyces cerevisiae Pho86p. Sequence analysis revealed an ORF of 860 bp encoding a 286-amino-acid protein with a predicted molecular mass of 32.8 kDa. This protein is shorter than its S. cerevisiae homologue, and is predicted to lack an ER-retention signal. Cell suspension work revealed that the null mutant is unable to take up nitrite from the medium.
Serrani
Federica
F
Laboratorio di Genetica Microbica, DiSA, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
Berardi
Enrico
E
D000595:Amino Acid Sequence; D001483:Base Sequence; D005800:Genes, Fungal / Q000502:physiology*; D008969:Molecular Sequence Data; D009573:Nitrites / Q000737:chemistry / Q000378:metabolism*; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism
D009573:Nitrites; D000644:Quaternary Ammonium Compounds