Paenibacillus wynnii sp. nov., a novel species harbouring the nifH gene, isolated from Alexander Island, Antarctica.
Soil taken from 12 different locations at Mars Oasis on Alexander Island, Antarctica, yielded unidentified isolates of endospore-forming bacteria. Soil from four of the locations contained Gram-negative, facultatively anaerobic, motile rods that were able to grow at 4 degrees C and which formed ellipsoidal spores that lay paracentrally or subterminally in swollen or slightly swollen sporangia. All of the strains harboured the nitrogenase gene nifH. Phenotypic tests, amplified rDNA restriction analysis (ARDRA), fatty acid analysis and SDS-PAGE analysis suggested that the isolates represented a novel taxon of Paenibacillus. 16S rRNA gene sequence comparison supported the proposal of a novel species, Paenibacillus wynnii sp. nov. (type strain, LMG 22176(T)=CIP 108306(T)).
Rodríguez-Díaz
Marina
M
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
Lebbe
Liesbeth
L
Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Rodelas
Belén
B
Grupo de Microbiología Ambiental, Departamento de Microbiología, Campus de Cartuja s/n, Universidad de Granada, 18071 Granada, Spain.
Heyrman
Jeroen
J
Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
De Vos
Paul
P
Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Logan
Niall A
NA
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
D000864:Antarctic Regions; D004269:DNA, Bacterial / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006092:Gram-Negative Facultatively Anaerobic Rods / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping / Q000379:methods; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000502:physiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C029130:nitrogenase reductase