Analysis of amino acids and proteins using a poly(methyl methacrylate) microfluidic system.
Plastic microchips are very promising analytical devices for the high-speed analysis of biological compounds. However, due to its hydrophobicity, their surface strongly interacts with nonpolar analytes or species containing hydrophobic domains, resulting in a significant uncontrolled adsorption on the channel walls. This paper describes the migration of fluorescence-labeled amino acids and proteins using the poly(methyl methacrylate) microchip. A cationic starch derivative significantly decreases the adsorption of analytes on the channel walls. The migration time of the analytes was related to their molecular weight and net charge or pI of the analytes. FITC-BSA migrated within 2 min, and the theoretical plate number of the peak reached 480,000 plates/m. Furthermore, proteins with a wide range of pI values and molecular weights migrated within 1 min using the microchip.
Kato
Masaru
M
Department of Analytical Chemistry, School of Pharmaceutical Sciences and COE Program in the 21st Century, University of Shizuoka, Shizuoka, Japan.
Gyoten
Yukari
Y
Sakai-Kato
Kumiko
K
Nakajima
Tohru
T
Toyo'oka
Toshimasa
T
D000596:Amino Acids / Q000032:analysis*; D001120:Arginine / Q000032:analysis; D002412:Cations / Q000737:chemistry; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013213:Starch / Q000737:chemistry
D000596:Amino Acids; D002412:Cations; D011506:Proteins; D012710:Serum Albumin, Bovine; D013213:Starch; D019904:Polymethyl Methacrylate; D001120:Arginine; D016650:Fluorescein-5-isothiocyanate