Selective photo-reduction of N-nitroamines combined with micellar electrokinetic chromatography and laser fluorimetric detection.
N-nitroso compounds (NOC) are potent carcinogens. Reliable methods for the analysis of volatile carcinogenic NOC are well established; however selective and sensitive methods for routine analysis of thermally unstable, ionic or non-volatile NOC are still needed. For this purpose, a method based on micellar electrokinetic chromatography (MEKC) with laser induced fluorescence (LIF) detection is described for the simultaneous determination of a broad range of N-nitroso compounds. In this procedure, the nitroso group is photolytically cleaved from the NOC to yield the corresponding amine. The amines are then derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl), identified and quantified using MEKC-LIF. For the standard mixture of NOC, this method has good sensitivity and a large dynamic range. The detection limit provided by the method is 9 ppb for N-nitrosopyrrolidine.
Bell
LeAnn M
LM
Hewlett Packard Company, 1000 NE Circle Blvd., Corvallis, OR 97330, USA. leann.bell@hp.com
Murray
George M
GM
D009327:4-Chloro-7-nitrobenzofurazan / Q000737:chemistry; D000588:Amines / Q000737:chemistry* / Q000528:radiation effects; D002273:Carcinogens / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005470:Fluorometry / Q000379:methods; D007834:Lasers; D009603:Nitroso Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D010782:Photolysis; D011759:Pyrrolidines / Q000737:chemistry; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
D000588:Amines; D002273:Carcinogens; D009603:Nitroso Compounds; D011759:Pyrrolidines; D009327:4-Chloro-7-nitrobenzofurazan