[Effects of Lycium barbarum polysaccharide on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing mice].
OBJECTIVE
To study the effects of Lycium barbarum polysaccharide (LBP) on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing mice and the mechanisms for intervention of tumor immune escape by LBP.


METHODS
H22-bearing mice were given LBP orally for two weeks. T-lymphocyte subsets and the phenotypes of dendritic cells in tumor-infiltrating lymphocytes (TIL) were detected by flow cytometry (FCM).


RESULTS
LBP could significantly increase the numbers of CD4(+) and CD8(+) T cells in TIL as compared with those in model control group (P<0.05). In model control group, the number of dendritic cells in tumor microenvironment decreased markedly, while in LBP-treated group, the increased number of dendritic cells and B7-1 expression were observed, but there were no significant differences between these two groups.


CONCLUSIONS
LBP has anti-tumor effect probably by increasing the numbers of CD4(+) and CD8(+) T cells in TIL to relieve the immunosuppression and enhance the anti-tumor function of the immune system. But whether LBP can recover the phenocyte and function of dendritic cells in H22-bearing mice should be further studied.
He
Yan-Li
YL
Department of Pathology, Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong Province 510405, China. Yahlihe16@163.com
Ying
Yi
Y
Xu
Yan-Li
YL
Su
Jun-Fang
JF
Luo
Hui
H
Wang
Hui-Feng
HF
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D003713:Dendritic Cells / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D008114:Liver Neoplasms, Experimental / Q000276:immunology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008297:Male; D051379:Mice; D016176:T-Lymphocyte Subsets / Q000187:drug effects*
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; C500223:lycium barbarum polysaccharide