It works: combinatorial biosynthesis for generating novel glycosylated compounds.
Combinatorial biosynthesis is a valuable method to generate novel glycosylated natural products. By coexpression of deoxysugar gene cassettes and genes from the staurosporine biosynthetic gene cluster it has now been applied to the generation of novel staurosporine derivatives. The work of Salas and co-workers is highlighted in this article.
Luzhetskyy
Andriy
A
Albert-Ludwigs-Universität, Institut für Pharmazeutische Wissenschaften, Pharmazeutische Biologie und Biotechnologie, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
Bechthold
Andreas
A
D002240:Carbohydrate Sequence; D003001:Cloning, Molecular; D020650:Combinatorial Chemistry Techniques; D005964:Glucosyltransferases / Q000235:genetics; D006027:Glycosides / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D019311:Staurosporine / Q000031:analogs & derivatives / Q000096:biosynthesis*; D013678:Technology, Pharmaceutical
D006027:Glycosides; D005964:Glucosyltransferases; D019311:Staurosporine