[Effect of supernatants of bone marrow mononuclear cells on endothelial cell proliferation].
OBJECTIVE
To define in vitro whether bone marrow mononuclear cells (BM-MNC) secrete vascular endothelial growth factor (VEGF) and to evaluate the effect of BM-MNC on endothelial cell proliferation.


METHODS
Rat BM-MNCs were isolated by centrifugation through a histopaque density gradient. BM-MNCs were cultured 4 weeks in vitro. Conditioned medium was assayed for VEGF and was added to cultured human umbilicus vein endothelial cells (HUVEC) to assess the effect of BM-MNC on endothelial cell (EC) proliferation.


RESULTS
Levels of VEGF in the conditioned medium increased compared with those in the control culture medium which was not exposed to BM-MNC. The conditioned medium enhanced, in a dose-related manner, HUVEC proliferation.


CONCLUSIONS
BM-MNCs is a valuable source of factors involved in angiogenesis. BM-MNCs secrete potent angiogenic cytokines such as VEGF and induce proliferation of endothelial cells.
Liu
Wen-Liang
WL
Department of Thoracic and Cardiovascular Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China. liuwenliang1859@hotmail.com
Ying
Bang-Liang
BL
Yu
Feng-Lei
FL
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014471:Umbilical Veins / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000494:pharmacology*
D042461:Vascular Endothelial Growth Factor A