Production and partial characterization of extracellular proteinases from Streptomyces malaysiensis, isolated from a Brazilian cerrado soil.
Streptomyces malaysiensis AMT-3, isolated from a Brazilian cerrado soil, showed proteolytic activities detected by gelatin-sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimum proteinase production was obtained when using 2.5% wheat bran and 0.1% yeast extract in the culture medium, after 5 days incubation at 30 degrees C. The enzymatic complex degraded gelatin optimally at pH 7.0, and under these conditions eight proteolytic bands (four serine-proteinases and four metaloproteinases), ranging from 20 to 212 kDa, were detected on the culture supernatant filtrates. In addition, a 35-kDa proteinase was thermostable at 60 degrees C for 120 min. These results point out to the applicability of gelatin zymograms in the characterization of crude enzymatic complexes. According to our results, this enzymatic complex could be used for biotechnological applications.
Nascimento
Rodrigo P
RP
Dep. Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, CCS, Universidade Federal do Rio de Janeiro (UFRJ), CEP, 21941-590, Rio de Janeiro, Brazil. rodrigopmbio@aol.com
d'Avila-Levy
Claudia M
CM
Souza
Rodrigo F
RF
Branquinha
Marta H
MH
Bon
Elba P S
EP
Pereira
Nei
N
Coelho
Rosalie R R
RR
D001938:Brazil; D003470:Culture Media / Q000737:chemistry; D004043:Dietary Fiber / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D005780:Gelatin / Q000378:metabolism; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000378:metabolism; D010461:Peptones / Q000378:metabolism; D012988:Soil Microbiology; D013302:Streptomyces / Q000201:enzymology* / Q000302:isolation & purification; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D004043:Dietary Fiber; D010461:Peptones; D005780:Gelatin; D010447:Peptide Hydrolases