title: The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by 31P NMR spectroscopy.
abstract: The effect of anoxia and the respiratory chain inhibitors azide and cyanide on the polyphosphate content of Phycomyces was studied by in vivo (31)P NMR spectroscopy. Anoxia was manifested by a decrease of core polyphosphates (PP(i)) and increase of intracellular inorganic phosphate (P(i)) signal. Normalized changes in PP(i)/P(i) ratio between control and nitrogen-purged mycelia suggest that the sensitivity to anoxia differs with growth phases. Azide acts in the same way as anoxia, by decreasing the PP(i)/P(i) ratio, while cyanide causes an increase of the PP(i)/P(i) ratio.
authors.affiliation: Institute of General and Physical Chemistry, Belgrade, Serbia. jzakrzewska@iofh.bg.ac.yu
authors.forename: Joanna
authors.initials: J
authors.lastname: Zakrzewska
authors.forename: Milan
authors.initials: M
authors.lastname: Zizić
authors.forename: Miroslav
authors.initials: M
authors.lastname: Zivić
mesh_terms: D001386:Azides / Q000494:pharmacology*; D003486:Cyanides / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D000860:Hypoxia / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D010759:Phosphorus Isotopes; D010800:Phycomyces / Q000187:drug effects* / Q000502:physiology; D011122:Polyphosphates / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001386:Azides; D003486:Cyanides; D004791:Enzyme Inhibitors; D010759:Phosphorus Isotopes; D011122:Polyphosphates; D009584:Nitrogen