1H nuclear magnetic resonance spectroscopy-based studies of the metabolism of food-borne carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline by human intestinal microbiota.
2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) is a mutagenic/carcinogenic compound formed from meat and fish during cooking. Following ingestion, IQ is metabolized mainly by liver xenobiotic-metabolizing enzymes, but intestinal bacteria may also contribute to its biotransformation. The aim of this study was to investigate the metabolism of IQ by the human intestinal microbiota. Following incubation of IQ (200 microM) under anoxic conditions with 100-fold dilutions of stools freshly collected from three healthy volunteers, we quantified residual IQ by high-pressure liquid chromatography (HPLC) analysis and characterized the production of IQ metabolites by in situ (1)H nuclear magnetic resonance ((1)H-NMR) spectroscopic analysis of crude incubation media. In addition, we looked for IQ-degrading bacteria by screening collection strains and by isolating new strains from the cecal contents of human-microbiota-associated rats gavaged with IQ on a regular basis. HPLC and (1)H-NMR analyses showed that the three human microbiota degraded IQ with different efficiencies (range, 50 to 91% after 72 h of incubation) and converted it into a unique derivative, namely, 7-hydroxy-IQ. We found 10 bacterial strains that were able to perform this reaction: Bacteroides thetaiotaomicron (n = 2), Clostridium clostridiiforme (n = 3), Clostridium perfringens (n = 1), and Escherichia coli (n = 4). On the whole, our results indicate that bacteria belonging to the predominant communities of the human intestine are able to produce 7-hydroxy-IQ from IQ. They also suggest interindividual differences in the ability to perform this reaction. Whether it is a metabolic activation is still a matter of debate, since 7-hydroxy-IQ has been shown to be a direct-acting mutagen in the Ames assay but not carcinogenic in laboratory rodents.
Humblot
Christèle
C
Institut National de la Recherche Agronomique, Unité d'Ecologie et de Physiologie du Système Digestif, Bâtiment Jacques Poly, 78352 Jouy-en-Josas, France.
Combourieu
Bruno
B
Väisänen
Marja-Liisa
ML
Furet
Jean-Pierre
JP
Delort
Anne-Marie
AM
Rabot
Sylvie
S
D000328:Adult; D000818:Animals; D001439:Bacteroides / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism*; D002432:Cecum / Q000382:microbiology; D003013:Clostridium / Q000378:metabolism*; D017077:Culture Media, Conditioned; D004926:Escherichia coli / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005502:Food; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D011804:Quinolines / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D017077:Culture Media, Conditioned; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline