[Study on HPLC fingerprint of Swertia mussotii var. mussotii from north-west area of Sichuan Province I].
OBJECTIVE
To constitute HPLC fingerprint of the methanol extract from Swertia mussotii grown in Sichuan Province.


METHODS
RP-HPLC, methanol and water including 0.02% acid as mobile phase, gradient elution, flow rate 1.0 mL x min(-1), the detection wavelength was 260 nm, temperature was 30 degrees C.


RESULTS
The RSD values of peak area and retention time of common peaks in precision, repeatability and stability were lower than 5.0%, respectively, similarity was over 0.805 in S. mussotii collected from 10 different habitats.


CONCLUSIONS
All results above exhibit that this method is simple, practicable, and reliable as a standard method in controlling the quality of S. mussotii.
Ji
Wen-he
WH
Northwest Institute of Plateau Biology, Academia Sinica, Xining 810001, China.
Lu
Xue-feng
XF
Chen
Gui-chen
GC
Ji
Lan-ju
LJ
D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D029967:Swertia / Q000737:chemistry*