[Cleavage of the deoxyribozyme targeting Period1 mRNA in vitro and its effects on morphine-induced reward in mice].
OBJECTIVE
To study the cleavage of the deoxyribozyme targeting Period1 (Per1) mRNA in vitro and its effect on the opiate-induced reward in mice.


METHODS
The specific deoxyribozyme (DRz164) targeting Per1 mRNA was designed after MFold analysis and being synthesized chemically. The cleavage reactions containing DRz164 and Per1 RNA fragments transcripted in vitro were performed under certain conditions. DIG Nucleic Acid Detection Kit was used to detect the cleavage activity of DRz164. The morphine-induced reward in mice was observed in a conditioned place preference test after pretreatment with DRz164.


RESULTS
When DRz164 and Per1 RNA transcripts were incubated under certain conditions with 37 degrees C for 30, 60, 90, and 120 min, about 36.4%, 40.5%, 47.8%, 63% of Per1 RNA transcripts were cleaved by DRz164 respectively. After pretreated with the deoxyribozyme, mice didn't show a morphine-induced place preference.


CONCLUSIONS
DRz164 targeting per1 mRNA has the specific cleavage activity which increases with the time in vitro. Suppression of morphine-induced place preference occurs by pretreatment with the deoxyribozyme. Mice show a lack of morphine-induced reward, which alleviates the psychic dependence on the morphine.
Zhou
Wei
W
Department of Biomedical Engineering, School of West China Basic Medicine and Forensic, Sichuan University, Chengdu Sichuan, China.
Liu
Yan-you
YY
Wang
Yue-qi
YQ
Liu
Ying-hui
YH
Wang
Yu-hui
YH
Wang
Zheng-rong
ZR
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D018797:Cell Cycle Proteins; D021881:DNA, Catalytic / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009020:Morphine / Q000494:pharmacology*; D009021:Morphine Dependence / Q000188:drug therapy* / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D056950:Period Circadian Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019966:Substance-Related Disorders / Q000188:drug therapy* / Q000235:genetics; D013997:Time Factors
D018797:Cell Cycle Proteins; D021881:DNA, Catalytic; D009687:Nuclear Proteins; C539023:Per1 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D009020:Morphine