Muscle tissue oxygen tension and oxidative metabolism during ischemia and reperfusion.
BACKGROUND
Recent studies have shown a relationship between alterations in tissue oxygen metabolism and cellular changes following ischemia and reperfusion, such as energy store depletion and intracellular acidosis. The aim of this study was to evaluate the relationship between tissue energy metabolism and intramuscular tissue oxygen tension in the mobilized latissimus dorsi muscle.


METHODS
The latissimus dorsi muscle was raised in New Zealand white rabbits (n = 10, 2.5 +/- 0.5 kg). During 4 h of ischemia and 2 h of reperfusion, the intramuscular tissue oxygen tension (Licox PO2-microcatheter probe) and the status of phosphorylated muscle energy metabolites were measured using a high-field 31P-NMR spectrometer. Linear correlation was performed between 31P-NMR data and tissue oxygen tension readings.


RESULTS
The tissue oxygen tension (PO2) values correlated significantly with phosphocreatine (PCr) (r = 0.96, P < 0.001), beta-adenosin triphosphate (beta-ATP) (r = 0.64, P <0.01), and intracellular pH (r = 0.82, P <0.001).


CONCLUSIONS
On the basis of these findings, we conclude that the data provided by tissue oxygen tension measurement offer a real time minimally invasive estimate of muscle oxidative metabolism during ischemia and reperfusion.
Troitzsch
Dirk
D
Department of Cardiac and Thoracic Vascular Surgery, Philipps-University of Marburg/Lahn, Germany. Dirk.Troitzsch@gmx.de
Vogt
Sebastian
S
Abdul-Khaliq
Hashim
H
Moosdorf
Rainer
R
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D004734:Energy Metabolism / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010725:Phosphocreatine / Q000032:analysis; D011817:Rabbits; D015427:Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology
D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D010100:Oxygen