Method to detect functional estrogen receptor expression using estrogen receptor probing compound.
A novel method to detect functional estrogen receptor expression with estrogen receptor probing compound (ERPC) has been established. ERPC is composed of estradiol-BSA-biotin. ERPC can be used to detect functional estrogen receptor expression in both Western blot and immunohistochemistry. ERPC based techniques are non-radioactive, sensitive, relatively inexpensive, and can be used with all species. The use of both ERPC and ERalpha- or ERbeta-specific antibodies provides complementary information in characterizing estrogen receptors' expression and functional binding to ligand.
Liu
Hongbiao
H
Department of Neurology, Reed Neurological Research Center, University of California, Los Angeles, CA 90095, USA.
Ding
Yi
Y
Voskuhl
Rhonda R
RR
D015153:Blotting, Western; D001943:Breast Neoplasms / Q000737:chemistry; D004958:Estradiol / Q000031:analogs & derivatives* / Q000737:chemistry; D047628:Estrogen Receptor alpha / Q000032:analysis* / Q000096:biosynthesis*; D018226:Fibroadenoma / Q000737:chemistry; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D047628:Estrogen Receptor alpha; C505445:estradiol-bovine serum albumin-biotin; D012710:Serum Albumin, Bovine; D004958:Estradiol