Effects of bone marrow derived mesenchymal stem cells transplantation in acutely infarcting myocardium.
BACKGROUND
Cellular cardiomyoplasty (CCM) is considered to be a novel therapeutic approach for post-myocardial infarction (MI) heart failure. In this study, the functional effects of cultured mesenchymal stem cells (MSCs) transplantation and the associated histopathologic changes were evaluated in a rat model of MI.


METHODS
Rats were subjected to 5 h of coronary ligation followed by reperfusion and, 10 days after MI, animals were randomized into either the MSCs transplantation (MI-MSC, n=8) group or the control (n=8) group. Allogeneic MSCs (3x10(6) cells) or media were epicardially injected into the center and the border area of the infarct scar.


RESULTS
Four weeks after the MSCs transplantation, the echocardiogram showed preserved anterior regional wall motion and increases in fractional shortening in the MI-MSC heart relative to the control heart. Left ventricular (LV) end-diastolic pressure was smaller in the MI-MSC than in the control group. Implanted MSCs formed islands of cell clusters on the border of the infarct scar, and the cells were positively immunostained by sarcomeric alpha-actinin and cardiac troponin T. In addition, the number of microvessels on the border area of the infarct scar was greater in the MI-MSC than in the control group.


CONCLUSIONS
Allogeneic MSCs transplanted into the MI scar formed clusters of cell grafts on the border of the infarct, expressed cardiac muscle proteins, increased microvessel formation, and improved regional and global LV function. Our data indicate that CCM using MSCs may have a significant role in the treatment of post-MI heart failure.
Piao
Hainan
H
Department of Internal Medicine, College of Medicine, Chungbuk National University, Cheongju, Korea.
Youn
Tae-Jin
TJ
Kwon
Jin-Sook
JS
Kim
Young-Hwa
YH
Bae
Jang-Whan
JW
Bora-Sohn
Kim
Dong-Woon
DW
Cho
Myeong-Chan
MC
Lee
Myoung-Mook
MM
Park
Young-Bae
YB
D000199:Actins / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004548:Elasticity; D006352:Heart Ventricles / Q000000981:diagnostic imaging; D007150:Immunohistochemistry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D009124:Muscle Proteins / Q000378:metabolism; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D020107:Troponin T / Q000378:metabolism; D014463:Ultrasonography; D016277:Ventricular Function, Left; D014841:von Willebrand Factor / Q000378:metabolism
D000199:Actins; D009124:Muscle Proteins; D020107:Troponin T; D014841:von Willebrand Factor