title: Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins.
abstract: BACKGROUND
  Many enzymes of industrial interest are not in the market since they are bio-produced as bacterial inclusion bodies, believed to be biologically inert aggregates of insoluble protein.
  
  
  RESULTS
  By using two structurally and functionally different model enzymes and two fluorescent proteins we show that physiological aggregation in bacteria might only result in a moderate loss of biological activity and that inclusion bodies can be used in reaction mixtures for efficient catalysis.
  
  
  CONCLUSIONS
  This observation offers promising possibilities for the exploration of inclusion bodies as catalysts for industrial purposes, without any previous protein-refolding step.
authors.affiliation: Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain. Elena.Garcia.Fruitos@uab.es
authors.forename: Elena
authors.initials: E
authors.lastname: García-Fruitós
authors.forename: Nuria
authors.initials: N
authors.lastname: González-Montalbán
authors.forename: Montse
authors.initials: M
authors.lastname: Morell
authors.forename: Andrea
authors.initials: A
authors.lastname: Vera
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Ferraz
authors.forename: Anna
authors.initials: A
authors.lastname: Arís
authors.forename: Salvador
authors.initials: S
authors.lastname: Ventura
authors.forename: Antonio
authors.initials: A
authors.lastname: Villaverde