title: In vivo visualization of cardiac allograft rejection and trafficking passenger leukocytes using bioluminescence imaging.
abstract: BACKGROUND
  We investigated the feasibility of bioluminescence imaging (BLI) for the in vivo assessment of cardiac allograft viability and visualization of passenger leukocytes during the course of acute rejection.
  
  
  RESULTS
  Hearts of FVB (H-2q) luciferase-green fluorescent protein transgenic mice (beta-actin promoter) or FVB luciferase transgenic mice (CD5 promoter) were heterotopically transplanted into either BALB/c (H-2d) or FVB recipients. Light intensity emitting from the recipient animals was measured daily by in vivo BLI until 12 days after transplantation. Graft beating score (0 to 4) was assessed by daily abdominal palpation until 12 days after transplantation. Inflammatory cell infiltration (CD45 stain) and structural changes of green fluorescent protein-positive cardiomyocytes were followed by immunohistochemistry. All cardiac allografts were acutely rejected by 12 days after transplantation. The intensity of light emitting from cardiac allografts declined 4 days after transplantation and correlated with graft beating scores (R2=0.91, P=0.02). Immunohistochemistry confirmed these results by showing an increase of CD45+ inflammatory cell infiltration and destruction of green fluorescent protein-positive cardiomyocytes in the cardiac allografts during acute rejection. In vivo BLI visualized migration and proliferation of CD5+ passenger leukocytes in both syngeneic and allogeneic recipients. In the allograft recipients, light signal from CD5+ passenger leukocytes peaked at 6 hours and diminished by 12 hours, whereas in the syngeneic recipients, the signal remained high until 10 days after transplantation.
  
  
  CONCLUSIONS
  BLI is a useful modality for the quantitative assessment of in vivo cardiac graft viability and tracking of passenger leukocytes in vivo during the course of acute rejection.
authors.affiliation: Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CA 94305-5407, USA. robbins@stanford.edu
authors.forename: Masashi
authors.initials: M
authors.lastname: Tanaka
authors.forename: Rutger-Jan
authors.initials: RJ
authors.lastname: Swijnenburg
authors.forename: Feny
authors.initials: F
authors.lastname: Gunawan
authors.forename: Yu-An
authors.initials: YA
authors.lastname: Cao
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Caffarelli
authors.forename: Jorg L
authors.initials: JL
authors.lastname: de Bruin
authors.forename: Cristopher H
authors.initials: CH
authors.lastname: Contag
authors.forename: Robert C
authors.initials: RC
authors.lastname: Robbins
mesh_terms: D000818:Animals; D018956:CD5 Antigens / Q000032:analysis; D002455:Cell Division; D019070:Cell Lineage; D005240:Feasibility Studies; D005260:Female; D005347:Fibroblasts / Q000637:transplantation; D049454:Firefly Luciferin; D017930:Genes, Reporter; D006084:Graft Rejection / Q000276:immunology / Q000473:pathology*; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D016027:Heart Transplantation / Q000276:immunology*; D008156:Luciferases / Q000235:genetics; D049449:Luminescence; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000637:transplantation*; D016042:Transplantation, Heterotopic; D014184:Transplantation, Homologous / Q000276:immunology / Q000473:pathology*; D014185:Transplantation, Isogeneic
chemical_list: D018956:CD5 Antigens; D049452:Green Fluorescent Proteins; D049454:Firefly Luciferin; D008156:Luciferases