Spectroscopic techniques applied to the study of laminin fragments inserted into model membranes.
The influence of four laminin-derived peptides on bilayer organization is studied. Spectroscopic methods applied were based on pyrene fluorescence properties (quenching, I1/I3, and monomer/excimer equilibrium), asymmetric membrane fluorescence (NBD-PE/dithionite), and polarization fluorescence (TMA-DPH). Also, the ability of these peptides to release carboxyfluorescein entrapped in vesicles was determined. Results suggest that these peptides do not noticeably modify the packing and motion of lipids (in the gel state), but coat its surface, preventing penetration of quenchers and chemical reactants. Nevertheless, their presence promotes a soft release of entrapped CF after incubation at 37 degrees C.
Crosas
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Peptides Department, Institute for Chemical and Environmental Research, Jordi Girona 18, 08034 Barcelona, Spain.
Egea
M A
MA
Reig
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F
D005454:Fluorescence Polarization; D036681:Fluorescence Recovery After Photobleaching; D005470:Fluorometry; D007797:Laminin / Q000737:chemistry*; D008567:Membranes, Artificial; D010446:Peptide Fragments / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D007797:Laminin; D008567:Membranes, Artificial; D010446:Peptide Fragments