The MHC-haplotype influences primary, but not memory, immune responses to an immunodominant peptide containing T- and B-cell epitopes of the caprine arthritis encephalitis virus Gag protein.
In this report, we describe a short peptide, containing a T helper- and a B-cell epitope, located in the Gag protein of the caprine arthritis encephalitis virus (CAEV). This T-cell epitope is capable of inducing a robust T-cell proliferative response in vaccinated goats with different genetic backgrounds and to provide help for a strong antibody response to the B-cell epitope, indicating that it may function as a universal antigen-carrier for goat vaccines. The primary immune response of goats homozygous for MHC class I and II genes showed an MHC-dependent partitioning in rapid-high and slow-low responses, whereas the memory immune response was strong in both groups, demonstrating that a vaccine based on this immunodominant T helper epitope is capable to overcome genetic differences.
Fluri
Alexandra
A
Institute of Veterinary Virology, University of Berne, Laenggass-Strasse 122, CH-3012 Berne, Switzerland.
Nenci
Chiara
C
Zahno
Marie-Luise
ML
Vogt
Hans-Rudolf
HR
Charan
Shiv
S
Busato
André
A
Pancino
Gianfranco
G
Peterhans
Ernst
E
Obexer-Ruff
Gabriela
G
Bertoni
Giuseppe
G
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000235:genetics / Q000276:immunology; D016088:Arthritis-Encephalitis Virus, Caprine / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D015683:Gene Products, gag / Q000276:immunology*; D006041:Goats / Q000276:immunology*; D006239:Haplotypes; D006433:Hemocyanins / Q000276:immunology; D007115:Immunization Schedule; D016056:Immunodominant Epitopes / Q000276:immunology*; D007156:Immunologic Memory / Q000235:genetics* / Q000276:immunology*; D008285:Major Histocompatibility Complex / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D017433:Protein Structure, Secondary; D013601:T-Lymphocytes / Q000276:immunology*
D015683:Gene Products, gag; D016056:Immunodominant Epitopes; D010455:Peptides; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin