title: [Promoter methylation profile in gastric cancer].
abstract: BACKGROUND
  Promoter genomic DNA methylation is an important inactivation mechanism of tumor suppressor genes. This genetic-molecular pathway for cancer may separate a subset of patients with different prognoses and eventually different responses to specific therapies.
  
  
  OBJECTIVE
  To analyze the methylation pattern of important genes related to different carcinogenic mechanisms in patients with gastric cancer (GC) and the relationship with its morphological features and biological behavior.
  
  
  METHODS
  Forty-seven fresh-frozen GC samples were selected. The methylation-specific PCR (MSP) test was used to analyze promoter methylation status for genes MLH1, CDKN2A (p16), APC, CDH1 (Cadherin E) and FHIT. Follow-up and complete morphological features were obtained for all cases.
  
  
  RESULTS
  We found methylation in at least one of the genes studied in 83% of the cases. The frequencies of promoter hypermethylation of MLH1, CDKN2A, APC, CDH1 and FHIT were 31%, 43%, 46%, 80% y 62%, respectively. We found a relationship between APC methylation and good histological differentiation (p =0.03); CDH1 methylation with diffuse type by Lauren and 3 or more metastasic lymph nodes (p <0.05); FHIT, CDKN2A and CDH1 methylation and female condition (p <0.04). We also found a non-significant relationship between CDKN2A methylation and better survival (p =0.07).
  
  
  CONCLUSIONS
  The high frequency promoter methylation found confirms its importance in gastric carcinogenesis. The finding of alterations in the methylation pattern of genes studied and its association with prognostic factors is a helpful tool in the search for new criteria in clinical and therapeutic decision making.
authors.affiliation: Departamento de Anatomía Patológica, Universidad de La Frontera, Temuco, Chile. jcroa@ufro.cl
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Roa
authors.forename: Leonardo
authors.initials: L
authors.lastname: Anabalón
authors.forename: Iván
authors.initials: I
authors.lastname: Roa
authors.forename: Oscar
authors.initials: O
authors.lastname: Tapia
authors.forename: Angélica
authors.initials: A
authors.lastname: Melo
authors.forename: Miguel
authors.initials: M
authors.lastname: Villaseca
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Araya
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019175:DNA Methylation; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics*