Characterization of an iodothyronine 5'-deiodinase in gilthead seabream (Sparus auratus) that is inhibited by dithiothreitol.
Iodothyronine deiodinases catalyze the conversion of the thyroid prohormone T(4) to T(3) by outer ring deiodination (ORD) of the iodothyronine molecule. The catalytic cycle of deiodinases is considered to be critically dependent on a reducing thiol cosubstrate that regenerates the selenoenzyme to its native state. The endogenous cosubstrate has still not been firmly identified; in studies in vitro the sulfhydryl reagent dithiothreitol (DTT) is commonly used to activate ORD. We now have characterized an ORD activity in the teleost gilthead seabream (Sparus auratus) that is inhibited by DTT. DTT inhibited reverse T(3) (rT(3)) ORD by 70 and 100% in kidney homogenates (IC(50) 0.4 mmol/liter) and microsomes (IC(50) 0.1 mmol/liter), respectively. The omission of DTT from the incubation medium restored renal ORD Michaelis-Menten kinetics with a Michaelis constant value of 5 mumol/liter rT(3) and unmasked the inhibition by 6-n-propyl-2-thiouracil. A putative seabream deiodinase type 1 (saD1), derived from kidney mRNA, showed high homology (> or = 41% amino acid identity) with vertebrate deiodinases type 1. Features of this putative saD1 include a selenocysteine encoded by an in-frame UGA codon, consensus sequences, and a predicted secondary structure for a selenocysteine insertion sequence and an amino acid composition of the catalytic center that is identical with reported consensus sequences for deiodinase type 1. Remarkably, three of six cysteines that are present in the deduced saD1 protein occur in the predicted amino terminal hydrophobic region. We suggest that the effects of DTT on rT(3) ORD can be explained by interactions with the cysteines unique to the putative saD1 protein.
Klaren
Peter H M
PH
Department of Organismal Animal Physiology, Institute for Neuroscience, Radboud University Nijmegen, The Netherlands. p.klaren@science.ru.nl
Haasdijk
Remco
R
Metz
Juriaan R
JR
Nitsch
Lisette M C
LM
Darras
Veerle M
VM
Van der Geyten
Serge
S
Flik
Gert
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics; D004229:Dithiothreitol / Q000494:pharmacology*; D007453:Iodide Peroxidase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D007455:Iodine / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D021541:Sea Bream / Q000378:metabolism*; D017279:Selenocysteine / Q000235:genetics; D014285:Triiodothyronine, Reverse / Q000037:antagonists & inhibitors / Q000378:metabolism
D003062:Codon; D018076:DNA, Complementary; D017279:Selenocysteine; D014285:Triiodothyronine, Reverse; D007455:Iodine; D007453:Iodide Peroxidase; D004229:Dithiothreitol