title: Histological assessment in grafts of highly purified beta-tricalcium phosphate (OSferion) in human bones.
abstract: Prominent osteoconductive activity and the biodegradable nature of commercially available beta-tricalcium phosphate (beta-TCP, OSferion) have been documented in animal experiments. We analyzed four cases of involving grafted OSferion in human bone with respect to histological features by routine hematoxylin and eosin staining, silver impregnation, immunohistochemistry and in situ hybridization. OSferion affords early bioresorption by osteoclasts, vascular invasion of macropores and osteoblastic cell attachment on the surface on the ceramic surface 14 days after grafting. Prominent bone formation and direct bone connection between preexisting bone and OSferion were evident 28 days after grafting. Nearly the entire TCP surface was covered by lamellar bone; additionally, active osteoblastic lining and attachment of the osteoclast-like giant cells were not observed 72 weeks after grafting. Silver impregnation revealed the presence of collagen fibrils within probable micropores of OSferion.
authors.affiliation: Division of Orthopedic Surgery, Graduate School of Medical and Dental Sciences, Niigata University, Asahimachi 1-751, Niigata 951-8510, Japan. aogose@med.niigata-u.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Ogose
authors.forename: Naoki
authors.initials: N
authors.lastname: Kondo
authors.forename: Hajime
authors.initials: H
authors.lastname: Umezu
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Hotta
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawashima
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Ito
authors.forename: Naoko
authors.initials: N
authors.lastname: Kudo
authors.forename: Makiko
authors.initials: M
authors.lastname: Hoshino
authors.forename: Wenguang
authors.initials: W
authors.lastname: Gu
authors.forename: Naoto
authors.initials: N
authors.lastname: Endo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001672:Biocompatible Materials / Q000302:isolation & purification / Q000627:therapeutic use; D018786:Bone Substitutes / Q000302:isolation & purification / Q000627:therapeutic use; D001842:Bone and Bones / Q000166:cytology* / Q000473:pathology; D002130:Calcium Phosphates / Q000302:isolation & purification / Q000627:therapeutic use; D017886:Durapatite; D005260:Female; D005264:Femoral Fractures / Q000473:pathology / Q000601:surgery; D006620:Hip Fractures / Q000473:pathology / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000473:pathology / Q000601:surgery
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D017886:Durapatite