Comparison of effect of vitamin E-coated dialyzer and oral vitamin E on hemodialysis-induced Cu/Zn-superoxide dismutase.
BACKGROUND
We reported earlier that production of Cu/Zn-superoxide dismutase (SOD) increases markedly in hemodialysis patients but not in non-dialyzed chronic renal failure (CRF) patients. In this study, we compared the antioxidant effects of oral vitamin E supplementation (VE-PO) and vitamin E coating of a dialyzer (VE-BMD) by measuring increased Cu/Zn-SOD in hemodialysis patients.


METHODS
31 hemodialysis patients were divided into two groups: 16 hemodialysis patients underwent usual dialysis with vitamin E supplementation 600 mg/day while 15 others were dialyzed using vitamin E-coated membrane for 6 months. Total plasma SOD activity was determined by NBT method, plasma Cu/Zn-SOD contents by ELISA and Cu/Zn-SOD mRNA in leukocytes by RT-PCR.


RESULTS
VE-PO and VE-BMD showed almost comparable effects on Cu/Zn-SOD contents and its mRNA levels in hemodialysis patients. VE-PO resulted in a progressive decrease of Cu/Zn-SOD content (p < 0.001). A comparable progressive decrease was observed also in VE-BMD (p < 0.0001). Both VE-PO and VE-BMD resulted in a progressive decrease of Cu/Zn-SOD mRNA (p < 0.01), which reached the level of non-dialyzed CRF patients.
Akiyama
Shinichiro
S
Department of Pharmacology, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. ANC18271@nifty.com
Inagaki
Masahiro
M
Tsuji
Mayumi
M
Gotoh
Hiromichi
H
Gotoh
Tomomi
T
Washio
Kazunori
K
Gotoh
Yoshikazu
Y
Oguchi
Katsuji
K
D000284:Administration, Oral; D000975:Antioxidants / Q000008:administration & dosage* / Q000627:therapeutic use; D020099:Coated Materials, Biocompatible; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy*; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood; D006435:Renal Dialysis / Q000295:instrumentation*; D013482:Superoxide Dismutase / Q000097:blood* / Q000235:genetics; D014810:Vitamin E / Q000008:administration & dosage* / Q000627:therapeutic use
D000975:Antioxidants; D020099:Coated Materials, Biocompatible; D012333:RNA, Messenger; D014810:Vitamin E; D013482:Superoxide Dismutase