title: Optimization of two flow-injection spectrophotometric methods for the determination of indapamide in pharmaceutical dosage forms.
abstract: Multivariate experimental design has been used to optimize 2 flow-injection spectrophotometric methods for the determination of indapamide in pharmaceutical dosage forms, both pure and commercial tablets. The methods are based on the oxidation of this drug with iron (III) in acidic medium and the subsequent formation of an intensive orange-red complex between the liberated iron (II) and 2,2'-bipyridyl or 1,10-phenanthroline reagents. Plackett-Burman designs were applied as a screening method to evaluate the most significant factors with few experiments. Central composite 2(3)+ star designs were performed to evaluate the response surfaces. The methods have been fully validated and were applied successfully to the determination of indapamide in pure and pharmaceutical forms with good accuracy and precision. Therefore, the 2 proposed procedures are simple, inexpensive, and rapid flow methods for the routine determination of indapamide in pharmaceutical preparations.
authors.affiliation: Universidad de Santiago de Compostela, Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Ciencias, Alfonso X El Sabio s/n, Lugo, 27002, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Rodríguez
authors.forename: Julia
authors.initials: J
authors.lastname: Barciela
authors.forename: Sagrario
authors.initials: S
authors.lastname: García
authors.forename: Carlos
authors.initials: C
authors.lastname: Herrero
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Peña
mesh_terms: D000959:Antihypertensive Agents / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical / Q000295:instrumentation / Q000379:methods; D003201:Computers; D004304:Dosage Forms; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000032:analysis; D007190:Indapamide / Q000032:analysis*; D007501:Iron / Q000032:analysis*; D008956:Models, Chemical; D004364:Pharmaceutical Preparations; D010618:Phenanthrolines / Q000032:analysis; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000295:instrumentation* / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013607:Tablets; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods; D014465:Ultrasonics
chemical_list: D000959:Antihypertensive Agents; D004304:Dosage Forms; D004364:Pharmaceutical Preparations; D010618:Phenanthrolines; D013607:Tablets; D000431:Ethanol; D007501:Iron; D007190:Indapamide; C025205:1,10-phenanthroline