title: Monitoring DNA structures by dual fluorescence of pyrene derivatives.
abstract: We have developed a nucleotide modified by a pyrene derivative with dual fluorescence. The dual fluorescence of the fluorophore, which was incorporated into DNA, was effectively controlled at ambient temperature according to DNA structural status. Our nucleoside with dual fluorescence is effective as a conceptually new probe for monitoring DNA hybridization by the color change without multilabeling with fluorescent dyes.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 615-8510, Japan. okamoto@sbchem.kyoto-u.ac.jp
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tainaka
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Nishiza
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
mesh_terms: D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D011721:Pyrenes / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature
chemical_list: D011721:Pyrenes; D004247:DNA