title: State and diagnostic value of plasma tissue factor in early-hospitalised patients with chest pain.
abstract: To study the state and diagnostic value of plasma tissue factor (TF) in patients with acute coronary syndromes (ACS), we quantitatively compared plasma TF antigen and TF activity in 90 early-hospitalised patients with chest pain. Using high-affinity antibodies, a sensitive assay for TF antigen was developed with a detection limit of 40 fmol/l. One of the antibodies was used to capture TF from plasma and, after elution and dialysis-free reconstitution in phospholipid-glucoside micelles, absolute amounts of TF activity could be measured with a detection limit of 80 fmol/l. All TF in plasma was found to be exposed, and a value of 2.5(1.1-14.8) pmol/l (median with range) was found for TF antigen. Most of this TF antigen (70-80%) circulated in a (potentially) functional state. Left in its in vivo state, however, TF captured from plasma was totally inactive, probably because of the lack of a procoagulant matrix. Compared with controls with non-cardiac chest pain, TF activity was unchanged and TF antigen about 25% elevated in ACS patients. Combined with the markers prothrombin fragment F1+2 and fatty acid-binding protein, TF did not improve the early diagnosis of ACS.
authors.affiliation: Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands.
authors.forename: Roy F M
authors.initials: RF
authors.lastname: van der Putten
authors.forename: Henk Te
authors.initials: HT
authors.lastname: te Velthuis
authors.forename: Chris
authors.initials: C
authors.lastname: de Zwaan
authors.forename: Lucien A
authors.initials: LA
authors.lastname: Aarden
authors.forename: Jan F C
authors.initials: JF
authors.lastname: Glatz
authors.forename: Wim T
authors.initials: WT
authors.lastname: Hermens
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000941:Antigens / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002637:Chest Pain / Q000097:blood*; D004562:Electrocardiography; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006760:Hospitalization; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood*; D011237:Predictive Value of Tests; D012372:ROC Curve; D013925:Thromboplastin / Q000032:analysis* / Q000276:immunology; D020107:Troponin T / Q000097:blood
chemical_list: D000941:Antigens; D015415:Biomarkers; D020107:Troponin T; D013925:Thromboplastin