title: Antiproliferative and antimitotic effect, S phase accumulation and induction of apoptosis and necrosis after treatment of extract from Rhodiola rosea rhizomes on HL-60 cells.
abstract: Rhodiola rosea is a medicinal plant having stimulating and adaptogenic properties, and some reports also indicate its anticancer and antimutagenic effect. However, the mechanism of its anticancer effect is unknown as there have been no cytological studies regarding cytostatics, cell cycle, induction of apoptosis or the mitotic activity of healthy and cancerous cells. In the present paper, those parameters were investigated using HL-60 cells, with flow cytometry and fluorescence microscopy. It has been found that the extract of Rhodiola rosea rhizomes inhibits division of HL-60 cells, which is preceded by an accumulation of cells at the prophase stage. This leads to induction of apoptosis and necrosis in HL-60 cells, and to marked reduction of their survival. The cells enter apoptosis from phase G2/M of the cell cycle. After treatment with the extract, no chromosome aberrations or micronuclei were observed, which indicates the mild action of the extract. The cytostatic and antiproliferative effect of the Rhodiola rosea rhizome extract, and its mild action, raises hope for its use in anticancer therapy by enhancing the effectiveness of cytostatics.
authors.affiliation: Department of Plant Morphogenesis, Warsaw University, Poland. AgaMajewska@biol.uw.edu.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Majewska
authors.forename: Grazyna
authors.initials: G
authors.lastname: Hoser
authors.forename: Hoser
authors.initials: H
authors.lastname: Grazyna
authors.forename: Mirosława
authors.initials: M
authors.lastname: Furmanowa
authors.forename: Furmanowa
authors.initials: F
authors.lastname: Mirosława
authors.forename: Natalia
authors.initials: N
authors.lastname: Urbańska
authors.forename: Urbańska
authors.initials: U
authors.lastname: Natalia
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Pietrosiuk
authors.forename: Pietrosiuk
authors.initials: P
authors.lastname: Agnieszka
authors.forename: Alicja
authors.initials: A
authors.lastname: Zobel
authors.forename: Zobel
authors.initials: Z
authors.lastname: Alicja
authors.forename: Mieczysław
authors.initials: M
authors.lastname: Kuraś
mesh_terms: D050256:Antimitotic Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D008940:Mitotic Index; D009336:Necrosis; D010936:Plant Extracts / Q000494:pharmacology*; D029771:Rhodiola; D016196:S Phase / Q000187:drug effects*
chemical_list: D050256:Antimitotic Agents; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts