Energy metabolism in orchid bee flight muscles: carbohydrate fuels all.
The widely accepted idea that bees fuel flight through the oxidation of carbohydrate is based on studies of only a few species. We tested this hypothesis as part of our research program to investigate the size-dependence of flight energetics in Panamanian orchid bees. We succeeded in measuring rates of O(2) consumption and CO(2) production in vivo during hovering flight, as well as maximal activities (V(max) values) in vitro of key enzymes in flight muscle energy metabolism in nine species belonging to four genera. Respiratory quotients (ratios of rates of CO(2) production to O(2) consumption) in all nine species are close to 1.0. This indicates that carbohydrate is the main fuel used for flight. Trehalase, glycogen phosphorylase and hexokinase activities are sufficient to account for the glycolytic flux rates estimated from rates of CO(2) production. High activities of other glycolytic enzymes, as well as high activities of mitochondrial oxidative enzymes, are consistent with the estimated rates of carbohydrate-fueled oxidative metabolism. In contrast, hydroxyacylCoA dehydrogenase, an enzyme involved in fatty acid oxidation, was not detectable in any species. Thoracic homogenates displayed ADP-stimulated oxidition of pyruvate + proline, but did not oxidize palmitoyl l-carnitine + proline as substrates. A metabolic map, based on data reported herein and information from the literature, is presented. The evidence available supports the hypothesis that carbohydrate serves as the main fuel for flight in bees.
Suarez
Raul K
RK
Department of Ecology, Evolution and Marine Biology, University of California Santa Barbara, Santa Barbara, CA 93106-9610, USA. suarez@lifesci.ucsb.edu
Darveau
Charles-A
CA
Welch
Kenneth C
KC
O'Brien
Diane M
DM
Roubik
David W
DW
Hochachka
Peter W
PW
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000818:Animals; D001516:Bees / Q000201:enzymology / Q000502:physiology*; D050260:Carbohydrate Metabolism / Q000502:physiology*; D002245:Carbon Dioxide / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D005426:Flight, Animal / Q000502:physiology*; D024981:Glycogen Phosphorylase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009132:Muscles / Q000201:enzymology / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology; D010176:Panama; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D014198:Trehalase / Q000378:metabolism
D002245:Carbon Dioxide; D015094:3-Hydroxyacyl CoA Dehydrogenases; D024981:Glycogen Phosphorylase; D006593:Hexokinase; D014198:Trehalase