[Effect of ecoimmunonutrition on gut barrier function in rats with systemic inflammatory response syndrome].
OBJECTIVE
To explore the effect of ecoimmunonutrition on gut barrier function in rats with systemic inflammatory response syndrome (SIRS).


METHODS
Rat models of SIRS were established by injecting lipo polysaccharide (LPS) via the tail vein. Sixty male Sprague-Dawley rats were randomly divided into 4 groups. One of the four enteral nutrition formulas (standard EN, immunonutrition, econutrition, ecoimmunonutrition) was given to each group respectively for 7 days. Serum D-lactate, diamine oxidase (DAO), ratio of urinary lactulose to mannitol (L/M) were measured on day 1 before LPS injection (D (-1)) and day 1, 3, 6 after LPS injection (D(+1), D (+3), D (+6)).


RESULTS
After 7 days of treatment,the level of serum D-lactate in econutrition group was significantly lower than those in standard EN or immunonutrition group (P< 0.05), and the level of serum D-lactate in all above three groups were significantly higher compared with ecoimmunonutrition group (P< 0.01). The level of serum DAO in standard EN group was higher than those in other three groups(P< 0.01). The L/M was increased in all the groups on D(+1), D(+3). It fell back to the basic level on D(+6)) in all the groups.The L/M in immunonutrition group was lower than those in other groups on D (+1) (P< 0.05), and it was higher in standard EN group than those in other groups on D (+3) (P< 0.01).


CONCLUSIONS
Ecoimmunonutrition, a enteral nutrition composed of immune-enhanced nutrient and ecologic, may protect the gut barrier function more effectively in rats with SIRS.
Peng
Jun-sheng
JS
Department of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. pengjunsheng@tom.com
Zhao
Xiao-lei
XL
Zhan
Wen-hua
WH
Zhao
Zong-gang
ZG
Yin
Yong
Y
D000818:Animals; D004195:Disease Models, Animal; D004750:Enteral Nutrition; D005973:Glutamine / Q000097:blood; D007413:Intestinal Mucosa / Q000503:physiopathology*; D007792:Lactulose / Q000097:blood; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood / Q000503:physiopathology* / Q000628:therapy*
D005973:Glutamine; D007792:Lactulose