[Determination of cholesteryl palmitate in Oviductus Ranae by HPLC].
OBJECTIVE
To develop a new method for the determination of cholesteryl palmitate in Oviductus Ranae.


METHODS
A HPLC method was set up, using Zorbax Silica column and cyclohexane-diethyl ether (40:1) as mobile phase with a flow rate of 1.0 mL x min(-1), and the UV detection wavelength was 203 nm.


RESULTS
The calibration curve was linear over the range of 0.60-8.92 microg (r = 0.9997), the average recovery of the method was 98.4%. RSD 1.8% (n = 6).


CONCLUSIONS
The results showed that method was reliable and accurate.
Wang
Yong-sheng
YS
Jilin University, Changchun 130021, China.
Jiang
Da-cheng
DC
Meng
Qin
Q
Wang
En-si
ES
D000818:Animals; D002788:Cholesterol Esters / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D008419:Materia Medica / Q000032:analysis* / Q000737:chemistry; D010057:Oviducts / Q000737:chemistry*; D011786:Quality Control; D011896:Rana temporaria
D002788:Cholesterol Esters; D008419:Materia Medica; C539466:Oviductus Ranae; C026651:cholesteryl palmitate