title: Determination of ambroxol hydrochloride, methylparaben and benzoic acid in pharmaceutical preparations based on sequential injection technique coupled with monolithic column.
abstract: The porous monolithic columns show high performance at relatively low pressure. The coupling of short monoliths with sequential injection technique (SIA) results in a new approach to implementation of separation step to non-separation low-pressure method. In this contribution, a new separation method for simultaneous determination of ambroxol, methylparaben and benzoic acid was developed based on a novel reversed-phase sequential injection chromatography (SIC) technique with UV detection. A Chromolith SpeedROD RP-18e, 50-4.6 mm column with 10 mm precolumn and a FIAlab 3000 system with a six-port selection valve and 5 ml syringe were used for sequential injection chromatographic separations in our study. The mobile phase used was acetonitrile-tetrahydrofuran-0.05M acetic acid (10:10:90, v/v/v), pH 3.75 adjusted with triethylamine, flow rate 0.48 mlmin(-1), UV-detection was at 245 nm. The analysis time was <11 min. A new SIC method was validated and compared with HPLC. The method was found to be useful for the routine analysis of the active compounds ambroxol and preservatives (methylparaben or benzoic acid) in various pharmaceutical syrups and drops.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, Hradec Králové 500 05, Czech Republic. satinsky@faf.cuni.cz
authors.forename: Dalibor
authors.initials: D
authors.lastname: Satínský
authors.forename: Jitka
authors.initials: J
authors.lastname: Huclová
authors.forename: Raquel L C
authors.initials: RL
authors.lastname: Ferreira
authors.forename: Maria Conceição B S M
authors.initials: MC
authors.lastname: Montenegro
authors.forename: Petr
authors.initials: P
authors.lastname: Solich
mesh_terms: D000551:Ambroxol / Q000032:analysis*; D019817:Benzoic Acid / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D005100:Expectorants / Q000032:analysis*; D010226:Parabens / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011310:Preservatives, Pharmaceutical / Q000032:analysis; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005100:Expectorants; D010226:Parabens; D004364:Pharmaceutical Preparations; D011310:Preservatives, Pharmaceutical; D000551:Ambroxol; D019817:Benzoic Acid; C015358:methylparaben