High-performance liquid chromatographic enantioseparation of flavanones on 2-hydroxy- 3-methacryloyloxypropyl beta-cyclodextrin copolymer coated silica phase.
Chromatographic resolution of four flavanones is achieved by reversed-phase high-performance liquid chromatography (HPLC) on a chiral stationary phase based on silica coated with a (2-hydroxy-3-methacryloyloxypropyl beta-cyclodextrin-co-N-vinylpyrrolidone) copolymer. The influence of the mobile phase water content and the nature of the organic modifier on the retention and resolution is evaluated. Monosubstituted flavanones are better resolved than the unsubstituted one. Nevertheless, the 6- and 7-methoxy substituents enhance retention and chiral recognition to polymeric beta-cyclodextrin stationary phase less than the 6-hydroxy group.
Carbonnier
Benjamin
B
Laboratoire de Chimie Macromoléculaire, UMR CNRS 8009, Université des Sciences et Technologies de Lille, F-59655 Cedex Villeneuve d'Ascq, France.
Janus
Ludovic
L
Morcellet
Michel
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones / Q000302:isolation & purification*; D008689:Methacrylates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011760:Pyrrolidinones / Q000737:chemistry; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry
D044950:Flavanones; D008689:Methacrylates; D011108:Polymers; D011760:Pyrrolidinones; D047392:beta-Cyclodextrins; D012822:Silicon Dioxide; C042670:N-vinyl-2-pyrrolidinone