A rapid screening test to distinguish between Candida albicans and Candida dubliniensis using NMR spectroscopy.
Nuclear magnetic resonance (NMR) spectroscopy combined with a statistical classification strategy (SCS) successfully distinguished between Candida albicans and Candida dubliniensis. 96% of the isolates from an independent test set were identified correctly. This proves that this rapid approach is a valuable method for the identification and chemotaxonomic characterisation of closely related taxa. Most discriminatory regions were correlated with metabolite profiles, indicating biochemical differences between the two species.
Himmelreich
Uwe
U
Centre for Infectious Diseases and Microbiology, ICPMR, University of Sydney at Westmead Hospital, Westmead, NSW 2145, Australia. himmelreich@mpin-koeln.mpg.de
Somorjai
Ray L
RL
Dolenko
Brion
B
Daniel
Heide-Marie
HM
Sorrell
Tania C
TC
D002175:Candida / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D002176:Candida albicans / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D016533:Mycological Typing Techniques