TNF-alpha antibody infusion impairs survival of stroke-generated neuroblasts in adult rat brain.
Stroke induced by 2 h middle cerebral artery occlusion triggers increased striatal and hippocampal neurogenesis in adult rats. We investigated the effect of tumor necrosis factor-alpha (TNF-alpha) inhibition on the survival of the new neurons. The mitotic marker BrdU was given on days 5 to 7, and TNF-alpha antibody or control protein was infused into the lateral ventricle of the ischemic hemisphere from day 8 to 14 after stroke. At the end of infusions, the TNF-alpha antibody-treated rats showed markedly fewer new striatal and hippocampal neurons, as compared to animals given control protein. The present findings suggest that TNF-alpha, probably acting via its receptor TNFR2, can promote the survival of stroke-generated hippocampal and striatal neurons.
Heldmann
Ursula
U
Laboratory of Neurogenesis and Cell Therapy, Section of Restorative Neurology, Wallenberg Neuroscience Center, University Hospital, SE-221 84 Lund, Sweden.
Thored
Pär
P
Claasen
Jan-Hendrik
JH
Arvidsson
Andreas
A
Kokaia
Zaal
Z
Lindvall
Olle
O
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology*; D007276:Injections, Intraventricular; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000276:immunology
D000906:Antibodies; D014409:Tumor Necrosis Factor-alpha