title: Homozygous carnitine palmitoyltransferase 1a (liver isoform) deficiency is lethal in the mouse.
abstract: To better understand carnitine palmitoyltransferase 1a (liver isoform, gene=Cpt-1a, protein=CPT-1a) deficiency in human disease, we developed a gene knockout mouse model. We used a replacement gene targeting strategy in ES cells that resulted in the deletion of exons 11-18, thus producing a null allele. Homozygous deficient mice (CPT-1a -/-) were not viable. There were no CPT-1a -/- pups, embryos or fetuses detected from day 10 of gestation to term. FISH analysis demonstrated targeting vector recombination at the expected single locus on chromosome 19. The inheritance pattern from heterozygous matings was skewed in both C57BL/6NTac, 129S6/SvEvTac (B6;129 mixed) and 129S6/SvEvTac (129 coisogenic) genetic backgrounds biased toward CPT-1a +/- mice (>80%). There was no sex preference with regard to germ-line transmission of the mutant allele. CPT-1a +/- mice had decreased Cpt-1a mRNA expression in liver, heart, brain, testis, kidney, and white fat. This resulted in 54.7% CPT-1 activity in liver from CPT-1a +/- males but no significant difference in females as compared to CPT-1a +/+ controls. CPT-1a +/- mice showed no fatty change in liver and were cold tolerant. Fasting free fatty acid concentrations were significantly elevated, while blood glucose concentrations were significantly lower in 6-week-old CPT-1a +/- mice compared to controls. Although the homozygous mutants were not viable, we did find some aspects of haploinsufficiency in the CPT-1a +/- mutants, which will make them an important mouse model for studying the role of CPT-1a in human disease.
authors.affiliation: Department of Genetics, University of Alabama at Birmingham, USA.
authors.forename: Lara R
authors.initials: LR
authors.lastname: Nyman
authors.forename: Keith B
authors.initials: KB
authors.lastname: Cox
authors.forename: Charles L
authors.initials: CL
authors.lastname: Hoppel
authors.forename: Janos
authors.initials: J
authors.lastname: Kerner
authors.forename: Barry L
authors.initials: BL
authors.lastname: Barnoski
authors.forename: Doug A
authors.initials: DA
authors.lastname: Hamm
authors.forename: Liqun
authors.initials: L
authors.lastname: Tian
authors.forename: Trenton R
authors.initials: TR
authors.lastname: Schoeb
authors.forename: Philip A
authors.initials: PA
authors.lastname: Wood
mesh_terms: D000818:Animals; D001483:Base Sequence; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D017931:DNA Primers; D005260:Female; D005804:Genes, Lethal; D006720:Homozygote; D017404:In Situ Hybridization, Fluorescence; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D002334:Carnitine O-Palmitoyltransferase