Genomic characterization and linkage mapping of the apple allergen genes Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin).
Four classes of apple allergens (Mal d 1, -2, -3 and -4) have been reported. By using PCR cloning and sequencing approaches, we obtained genomic sequences of Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin) from the cvs Prima and Fiesta, the two parents of a European reference mapping population. Two copies of the Mal d 2 gene (Mal d 2.01 A and Mal d 2.01 B) were identified, which primarily differed in the length of a single intron (378 or 380 nt) and in one amino acid in the signal peptide. Both Mal d 2.01 A and Mal d 2.01 B were mapped at identical position on linkage group 9. Genomic characterization of four Mal d 4 genes (Mal d 4.01 A and B, Mal d 4.02 A and Mal d 4.03 A) revealed their complete gDNA sequences which varied among genes in length from 862 to 2,017 nt. They all contained three exons of conserved length: 123, 138, and 135 nt. Mal d 4.01 appeared to be duplicated in two copies and located on linkage group 9. Mal d 4.02 A and Mal d 4.03 A were single copy genes located on linkage group 2 and 8, respectively.
Gao
Z S
ZS
Allergy Consortium Wageningen, Wageningen University and Research Centre, P.O. Box 16, 6700 Wageningen, The Netherlands.
Weg
W E van de
WE
Schaart
J G
JG
Arkel
G van
Gv
Breiteneder
H
H
Hoffmann-Sommergruber
K
K
Gilissen
L J W J
LJ
D000485:Allergens / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001947:Breeding / Q000379:methods; D002874:Chromosome Mapping; D017931:DNA Primers; D020131:Genes, Duplicate / Q000235:genetics; D018745:Genome, Plant; D006023:Glycoproteins / Q000235:genetics*; D027845:Malus / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D051304:Profilins / Q000235:genetics*; D017422:Sequence Analysis, DNA
D000485:Allergens; D017931:DNA Primers; D006023:Glycoproteins; D010940:Plant Proteins; D051304:Profilins