title: Effect of indomethacin on electrical field stimulation-induced contractions of isolated transverse and longitudinal rat gastric fundus strips.
abstract: OBJECTIVE
  To study the effects of indomethacin on the isolated transverse and longitudinal rat gastric fundus strips.
  
  
  METHODS
  The strips were suspended in an organ bath containing oxygenated Krebs solution, and contractile responses to electrical field stimulation were recorded on a physiograph in an isotonic manner after administration of cumulative concentrations of indomethacin. The effects of indomethacin on the strips pretreated with K(ATP) channel modulators, diazoxide and glybenclamide were studied.
  
  
  RESULTS
  Treatment of the transverse strips with indomethacin resulted in a concentration-dependent inhibitory response. In longitudinal strips, biphasic responses were seen, which included a stimulatory response at low concentrations of indomethacin, followed by an inhibitory response at higher concentrations. Diazoxide pre-treatment inhibited the stimulatory response of longitudinal strips. Glybenclamide pre-treatment not only blocked inhibitory effect of the low concentrations of indomethacin on transverse strips, but also increased the amplitude of contractions. Moreover, the drug decreased the amplitude of contractions in longitudinal strips.
  
  
  CONCLUSIONS
  Responses of the isolated longitudinal and transverse rat gastric fundus strips to indomethacin are not similar, and are influenced by K(ATP) channel modulators.
authors.affiliation: Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Salimeh
authors.initials: S
authors.lastname: Afshin
authors.forename: Mansoor
authors.initials: M
authors.lastname: Keshavarz
authors.forename: Mahmood
authors.initials: M
authors.lastname: Salami
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mirershadi
authors.forename: Bijan
authors.initials: B
authors.lastname: Djahanguiri
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D004558:Electric Stimulation; D005748:Gastric Fundus / Q000187:drug effects* / Q000502:physiology*; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007213:Indomethacin