title: A dipalmitoylated lipoprotein from Mycoplasma pneumoniae activates NF-kappa B through TLR1, TLR2, and TLR6.
abstract: The pathogenesis of Mycoplasma pneumoniae infection is considered to be in part attributed to excessive immune responses. Recently, lipoproteins from mycoplasmas have been reported to induce NF-kappaB activation. In this study, we examined the ability of lipoproteins from M. pneumoniae to activate NF-kappaB, and the active component responsible for the NF-kappaB activation was identified. Lipid-associated membrane proteins from M. pneumoniae were found to induce NF-kappaB through TLR 2 in a human monocytic cell line, THP-1. The active component of the Lipid-associated membrane proteins was a subunit b of F0F1-type ATPase (F0F1-ATPase). The F0F1-ATPase is assumed to contain two palmitic acids. The activation of NF-kappaB by the F0F1-ATPase was inhibited by a dominant negative construct of TLR1 and TLR6. These results indicate that the activation of NF-kappaB by F0F1-ATPase is dependent on TLR1, TLR2, and TLR6. The activity of the F0F1-ATPase was decreased with pretreatment of lipoprotein lipase but not protease, indicating that the lipid moiety of the F0F1-ATPase was important for the NF-kappaB activation. Thus, a dipalmitoylated lipoprotein from M. pneumoniae was found to activate NF-kappaB through TLR1, TLR2, and TLR6.
authors.affiliation: Department of Bacteriology, Kurume University School of Medicine, Kurume, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kida
authors.forename: Koichi
authors.initials: K
authors.lastname: Kuwano
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D025243:Bacterial Proton-Translocating ATPases / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D008074:Lipoproteins / Q000502:physiology*; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019308:Palmitic Acid / Q000378:metabolism*; D011019:Pneumonia, Mycoplasma / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D008074:Lipoproteins; D016328:NF-kappa B; D019308:Palmitic Acid; D025243:Bacterial Proton-Translocating ATPases