Antibody mediated transduction of therapeutic proteins into living cells.
Protein therapy refers to the direct delivery of therapeutic proteins to cells and tissues with the goal of ameliorating or modifying a disease process. Current techniques for delivering proteins across cell membranes include taking advantage of receptor-mediated endocytosis or using protein transduction domains that penetrate directly into cells. The most commonly used protein transduction domains are small cell-penetrating peptides derived from such proteins as the HIV-1 Tat protein. A novel protein transduction domain developed as the single chain fragment (Fv) of a murine anti-DNA autoantibody, mAb 3E10, has recently been developed and used to deliver biologically active proteins to living cells in vitro. This review will provide a brief overview of the development of the Fv fragment and provide a summary of recent studies using Fv to deliver therapeutic peptides and proteins (such as a C-terminal p53 peptide, C-terminal p53 antibody fragment, full-length p53, and micro-dystrophin) to cells.
Hansen
James E
JE
Department of Medicine, University of California, Los Angeles, CA 90095, USA. jameshansen2004@hotmail.com
Weisbart
Richard H
RH
Nishimura
Robert N
RN
D000818:Animals; D001323:Autoantibodies / Q000378:metabolism*; D001693:Biological Transport, Active / Q000502:physiology; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000379:methods*; D004705:Endocytosis / Q000502:physiology; D015696:Gene Products, tat / Q000276:immunology / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism; D006801:Humans; D007128:Immunoglobulin Fragments; D008222:Lymphokines / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D021381:Protein Transport / Q000502:physiology; D011506:Proteins / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D054322:tat Gene Products, Human Immunodeficiency Virus
D001323:Autoantibodies; C089422:Fv protein, human; D015696:Gene Products, tat; D007128:Immunoglobulin Fragments; D008222:Lymphokines; D010446:Peptide Fragments; D011506:Proteins; D012795:Sialoglycoproteins; D054322:tat Gene Products, Human Immunodeficiency Virus