Ribosome-inactivating proteins and other lectins from Adenia (Passifloraceae).
The caudices of 10 Adenia species contain galactose-binding lectins that were purified by affinity chromatography. All lectins but three agglutinate human erythrocytes. Six lectins consist of two unequal chains, which can be separated by reduction, and inhibit protein synthesis both by a rabbit reticulocyte lysate and by HeLa and Raji cells. The lectins from A. goetzii, A. lanceolata and A. stenodactyla had the highest cytotoxicity, inhibiting cell protein synthesis with IC50s (concentration inhibiting by 50%) below 0.1 ng/ml, and deadenylate DNA, thus being type 2 ribosome-inactivating proteins.
Pelosi
Emanuele
E
Dipartimento di Patologia sperimentale dell'Università di Bologna, Via S. Giacomo 14, I-40126 Bologna, Italy.
Lubelli
Chiara
C
Polito
Letizia
L
Barbieri
Luigi
L
Bolognesi
Andrea
A
Stirpe
Fiorenzo
F
D000818:Animals; D002460:Cell Line; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003601:Cytotoxicity Tests, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D031683:Passifloraceae / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification*; D010947:Plants, Toxic / Q000737:chemistry; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D011817:Rabbits; D012270:Ribosomes / Q000187:drug effects; D013045:Species Specificity; D014118:Toxins, Biological / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity
D037121:Plant Lectins; D011500:Protein Synthesis Inhibitors; D014118:Toxins, Biological