title: GLUT1 is highly expressed in cementoblasts but not in osteoblasts.
abstract: Cementum is a specialized mineralized tissue covering root surface of the tooth. Although the tissue's composition resembles bone, there are distinct structural and functional differences between the two mineralized tissues. In this study, the genes that are differentially expressed in putative cementoblasts (human cementum-derived cells [HCDCs]) compared with preosteoblastic cells (human bone marrow stromal cells [BMSCs]) were screened by two independent microarray systems, and some of the selected genes were further analyzed by quantitative real-time RT-PCR. The gene encoding glucose transporter 1 [GLUT1], which showed the greatest difference between the two groups by the latter analysis, was subjected to further analyses. High levels of the GLUT1 protein in HCDCs, but not in BMSCs, were detected by Western blotting and immunocytochemistry. Furthermore, intense immunoreactivities for GLUT1 were observed in cementoblasts and cementocytes but not in osteoblasts or osteocytes in human periodontal tissues. These results indicate that GLUT1 may play a role in cementogenesis and could serve as a biomarker to differentiate between cells of cementoblastic and osteoblastic lineage.
authors.affiliation: Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, Chiba, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Koike
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Uzawa
authors.forename: Wojciech J
authors.initials: WJ
authors.lastname: Grzesik
authors.forename: Naohiko
authors.initials: N
authors.lastname: Seki
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Endo
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kasamatsu
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Yamauchi
authors.forename: Hideki
authors.initials: H
authors.lastname: Tanzawa
mesh_terms: D000293:Adolescent; D015153:Blotting, Western; D002478:Cells, Cultured; D002648:Child; D003739:Dental Cementum / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics; D051272:Glucose Transporter Type 1; D006801:Humans; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495677:SLC2A1 protein, human