Coding and noncoding genomic regions of Entamoeba histolytica have significantly different rates of sequence polymorphisms: implications for epidemiological studies.
To evaluate genetic variability among Entamoeba histolytica strains, we sequenced 9,077 bp from each of 14 isolates. The polymorphism rates from coding and noncoding regions were significantly different (0.07% and 0.37%, respectively), indicating that these regions are subject to different selection pressures. Additionally, single nucleotide polymorphisms (SNPs) potentially associated with specific clinical outcomes were identified.
Bhattacharya
Dhruva
D
Department of Microbiology and Immunology, Division of Infectious Diseases, Stanford, CA 94305, USA.
Haque
Rashidul
R
Singh
Upinder
U
D000818:Animals; D004748:Entamoeba histolytica / Q000145:classification* / Q000235:genetics; D004749:Entamoebiasis / Q000453:epidemiology* / Q000469:parasitology; D016021:Epidemiologic Studies; D005260:Female; D014644:Genetic Variation; D018503:Genome, Protozoan; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D022661:RNA, Untranslated / Q000235:genetics*; D017422:Sequence Analysis, DNA
D022661:RNA, Untranslated