title: Improved electrochemical analysis of neuropathy target esterase activity by a tyrosinase carbon paste electrode modified by 1-methoxyphenazine methosulfate.
abstract: A graphite-paste tyrosinase biosensor was improved by adding 1-methoxyphenazine methosulfate as a mediator. Mediator modification enhanced sensitivity to phenol 4-fold and long-term stability 3-fold. Phenol could be detected at 25 nM (S/N = 2) using an Ag/AgCl reference electrode. The biosensor was used to measure the activity of a toxicologically significant enzyme, neuropathy target esterase (NTE), which yields phenol by hydrolysis of the substrate, phenyl valerate. Using the new biosensor, blood and brain NTE inhibition by organophosphorus (OP) compounds with different neuropathic potencies were well correlated (r = 0.990, n = 7), supporting the use of blood NTE as a biochemical marker of exposure to neuropathic OP compounds.
authors.affiliation: Faculty of Chemistry, Moscow State University, 119899, Moscow, Russia.
authors.forename: L G
authors.initials: LG
authors.lastname: Sokolovskaya
authors.forename: L V
authors.initials: LV
authors.lastname: Sigolaeva
authors.forename: A V
authors.initials: AV
authors.lastname: Eremenko
authors.forename: I V
authors.initials: IV
authors.lastname: Gachok
authors.forename: G F
authors.initials: GF
authors.lastname: Makhaeva
authors.forename: N N
authors.initials: NN
authors.lastname: Strakhova
authors.forename: V V
authors.initials: VV
authors.lastname: Malygin
authors.forename: R J
authors.initials: RJ
authors.lastname: Richardson
authors.forename: I N
authors.initials: IN
authors.lastname: Kurochkin
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002265:Carboxylic Ester Hydrolases / Q000032:analysis* / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D004789:Enzyme Activation; D008698:Mesylates / Q000737:chemistry*; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D009824:Ointments; D019800:Phenol / Q000032:analysis* / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D008698:Mesylates; D009824:Ointments; D019800:Phenol; D002244:Carbon; D014442:Monophenol Monooxygenase; D002265:Carboxylic Ester Hydrolases; C021859:neurotoxic esterase