PCR-based diagnosis of neonatal staphylococcal bacteremias.
We compared PCR with blood cultures in the diagnosis of neonatal staphylococcal sepsis. Significant association was observed between PCR-based and culture-based diagnosis of staphylococcal bacteremia. Positive and negative predictive values for PCR were 100% and 98%, respectively. These data indicate that PCR may serve as a useful adjunct for the rapid diagnosis of staphylococcal sepsis.
Makhoul
Imad R
IR
Department of Microbiology, Rambam Medical Center, Bat-Galim, Haifa, Israel.
Smolkin
Tatiana
T
Sujov
Polo
P
Kassis
Imad
I
Tamir
Ada
A
Shalginov
Raia
R
Sprecher
Hannah
H
D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D007223:Infant; D007230:Infant, Low Birth Weight; D007231:Infant, Newborn; D007234:Infant, Premature; D007235:Infant, Premature, Diseases / Q000175:diagnosis* / Q000382:microbiology; D007363:Intensive Care Units, Neonatal; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D003470:Culture Media; D004269:DNA, Bacterial