title: Extended sequence and functional analysis of the butirosin biosynthetic gene cluster in Bacillus circulans SANK 72073.
abstract: Butirosin produced by Bacillus circulans is among the clinically important 2-deoxystreptamine containing aminoglycoside antibiotics and its unique structure is found in (S)-4-amino-2-hydroxyburyric acid substituted at C-1 of 2-deoxystreptamine. Recently, the key part of the butirosin biosynthetic gene cluster has been identified from Bacillus circulans SANK 72073, however the whole gene for the biosynthesis awaited for identification. In the present study, we undertook extended analysis of the butirosin biosynthetic gene cluster and found nine additional open reading flames (ORFs), btrQ, btrR1, btrR2, btrT, btrU, btrV, btrW, btrX and orf1 in the cluster. In addition, we constructed disruption mutants of btrR1 and btrP-V, and found that the btr genes (ca. 24Kb) between btrR1 and btrP-V are at least required for the butirosin biosynthesis.
authors.affiliation: Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1, Meguro-ku, Tokyo 152-8551, Japan. eguchi@cms.titech.ac.jp
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Kudo
authors.forename: Mario
authors.initials: M
authors.lastname: Numakura
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tamegai
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Tadashi
authors.initials: T
authors.lastname: Eguchi
authors.forename: Katsumi
authors.initials: K
authors.lastname: Kakinuma
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001407:Bacillus / Q000235:genetics*; D015153:Blotting, Western; D002076:Butirosin Sulfate / Q000096:biosynthesis*; D016386:Chromosome Walking; D002876:Chromosomes, Bacterial / Q000235:genetics; D030541:Databases, Genetic; D005798:Genes, Bacterial / Q000235:genetics*; D005810:Multigene Family / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; C422952:telomerase RNA; D002076:Butirosin Sulfate; D012313:RNA; D019098:Telomerase