Identification of DNA sequences specific for Vibrio vulnificus biotype 2 strains by suppression subtractive hybridization.
Vibrio vulnificus can be divided into three biotypes, and only biotype 2, which is further divided into serovars, contains eel-virulent strains. We compared the genomic DNA of a biotype 2 serovar E isolate (tester) with the genomic DNAs of three biotype 1 strains by suppression subtractive hybridization and then tested the distribution of the tester-specific DNA sequences in a wide collection of bacterial strains. In this way we identified three plasmid-borne DNA sequences that were specific for biotype 2 strains irrespective of the serovar and three chromosomal DNA sequences that were specific for serovar E biotype 2 strains. These sequences have potential for use in the diagnosis of eel vibriosis caused by V. vulnificus and in the detection of biotype 2 serovar E strains.
Lee
Chung-Te
CT
Department of Microbiology and Immunology, College of Medicine, National Cheng-Kung University, Tainan 701, Taiwan, Republic of China.
Amaro
Carmen
C
Sanjuán
Eva
E
Hor
Lien-I
LI
D000818:Animals; D001483:Base Sequence; D004524:Eels / Q000382:microbiology; D005393:Fish Diseases / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012703:Serotyping; D013045:Species Specificity; D014735:Vibrio Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D041261:Vibrio vulnificus / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D014774:Virulence