The regenerative cells during the metamorphosis in the midgut of bees.
The midgut epithelium of bees is formed by the digestive cells, responsible for enzyme secretion and nutrient absorption and for small regenerative cells that are placed in nests scattered among the digestive cells. During metamorphosis, the larval midgut epithelium degenerates and a new adult midgut epithelium is built during larval differentiation of regenerative cells. The present work focuses on the midgut epithelial modifications during the post-embryonic development of the stingless bee Melipona quadrifasciata anthidioides worker and the occurrence of regenerative cell proliferation during midgut metamorphosis in order to test the hypothesis that adult midgut epithelium of worker bees results from regenerative cell proliferation during the pupal stage. Regenerative cell proliferation was detected during larval lifespan. Larval aging is followed by an increase in the number and the size of the nests of regenerative cells. Larval epithelium degeneration begins 2 days after the start of defecation process and in this period the nests of regenerative cells are in contact by means of cytoplasmic extension which have many septate desmosomes and gap junctions. The BrdU immunoreactive regenerative cells were found in the prepupae 12 h after BrdU injection, suggesting that regenerative cell population increase during this larval period. Regenerative cell proliferation results in the increase of the regenerative cell population and not in the formation of new digestive cells because the proliferation of regenerative cells would not be enough to reestablish the nests of regenerative cells and at the same time form new adult digestive cells. In this sense the hypothesis that digestive adult cells originate from regenerative cell proliferation during midgut metamorphosis in M. quadrifasciata anthidioides was rejected.
Martins
Gustavo Ferreira
GF
Departamento de Biologia Animal, Universidade Federal de Viçosa, Brazil.
Neves
Clóvis Andrade
CA
Campos
Lúcio Antonio Oliveira
LA
Serrão
José Eduardo
JE
D000818:Animals; D001516:Bees / Q000166:cytology* / Q000254:growth & development* / Q000648:ultrastructure; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation; D004064:Digestive System / Q000166:cytology / Q000254:growth & development / Q000648:ultrastructure; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology / Q000648:ultrastructure; D008675:Metamorphosis, Biological; D008853:Microscopy; D046529:Microscopy, Electron, Transmission; D013194:Staining and Labeling
D001973:Bromodeoxyuridine