Testing the hypothesis that system y(+)L accounts for high- and low-transport phenotypes in chicken erythrocytes using L-leucine as substrate.
Experiments were carried out to test the hypothesis that system y(+)L accounts for the high (HT) and low (LT) amino-acid transport phenotypes in chicken erythrocytes and to explain the different effect of selective breeding on lysine and leucine fluxes. L: -Leucine transport was characterized in individuals which had been separated into two groups (HT and LT) according to their capacity to transport L: -lysine across the erythrocyte membrane. Whereas lysine influx (1 muM: ) in the two groups differed by 32-fold (HT/LT), leucine influx was not significantly different. Average rates (nmol/ L cells/ min) were: 227 (HT) and 7.0 (LT) for L: -lysine, and 8.9 (HT) and 7.1 (LT) for L: -leucine. The differential ability of L: -lysine and L: -leucine fluxes to discriminate between the HT and LT phenotypes was shown to be consistent with the interactions of these substrates with system y(+)L and to vary depending on the conditions of the assay. It is shown that the two phenotypes can be clearly discriminated by measuring L: -leucine influx in the presence of Li(+). These results support the hypothesis that the HT and LT phenotypes reflect alterations in the function of system y(+)L and illustrate that the choice of the appropriate substrate and medium composition must be carefully considered when investigating the consequences of either experimental or natural alterations of broad-scope transporters.
Angelo
S
S
Programa de Fisiología y Biofísica, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 7, Casilla 70005 Chile.
Cabrera
S
S
Rojas
A M
AM
Rodríguez
N
N
Devés
R
R
D027241:Amino Acid Transport System y+L / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000502:physiology*; D002645:Chickens / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D008094:Lithium / Q000378:metabolism; D010641:Phenotype; D012964:Sodium / Q000378:metabolism
D027241:Amino Acid Transport System y+L; D008094:Lithium; D012964:Sodium; D007930:Leucine