Analysis of Esg1 expression in pluripotent cells and the germline reveals similarities with Oct4 and Sox2 and differences between human pluripotent cell lines.
Establishment of pluripotent epiblast cells is a critical event during early mammalian development because all somatic lineages and the primordial germ cells (PGCs) are derived from them. The epiblast and PGCs are in turn the precursors of pluripotent embryonic stem cells and embryonic germ cells, respectively. Although PGCs are specialized cells, they express several key pluripotency-related genes, such as Oct4 and Sox2. We have analyzed Esg1 expression in mouse and human cells and shown that in the mouse the gene is specifically expressed in preimplantation embryos, stem cells, and the germline. Moreover, Esg1 coexpresses with Oct4 and Sox2, confirming its identity as a marker of the pluripotent cycle. Esg1 is also expressed with Oct4 and Sox2 by human embryonic stem cells and in germ cell carcinoma tissue but not by all human embryonal carcinoma cell lines. These data suggest that together with Oct4 and Sox2, Esg1 plays a conserved role in the pluripotent pathway of mouse and human stem and germ cells.
Western
Patrick
P
ARC Centre of Excellence in Biotechnology and Development and Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Victoria, Australia.
Maldonado-Saldivia
Joanna
J
van den Bergen
Jocelyn
J
Hajkova
Petra
P
Saitou
Mitinori
M
Barton
Sheila
S
Surani
M Azim
MA
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D019070:Cell Lineage; D056970:Co-Repressor Proteins; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism*; D024002:HMGB Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D012097:Repressor Proteins; D055748:SOXB1 Transcription Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D000943:Antigens, Differentiation; D051792:Basic Helix-Loop-Helix Proteins; D056970:Co-Repressor Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D024002:HMGB Proteins; D050814:Octamer Transcription Factor-3; D012097:Repressor Proteins; D055748:SOXB1 Transcription Factors; D015534:Trans-Activators; D014157:Transcription Factors; C508649:Enhancer of Split Groucho protein, mouse; C527782:SOX2 protein, human; C527783:Sox2 protein, mouse; C499043:TLE1 protein, human