title: Dynamic study of calcium phosphate formation on porous HA/TCP ceramics.
abstract: Bone-like apatite formation on porous calcium phosphate ceramics was investigated in static simulated body fluid (SBF) and dynamic SBF at different flowing rates. The results of a 14-day immersion in static SBF showed that the formation of bone-like apatite occurred both on the surface and in the pores of the samples. When SBF flowed at the physiological flow rate in muscle (2 ml/100 ml.min), bone-like apatite could be detected only in internal surface of the pores of samples. The result that bone-like apatite formation could only be found in the pores when SBF flowed at physiological flow rate was consistent with that of porous calcium phosphate ceramics implanted in vivo: osteoinduction was only detected inside the pores of the porous calcium phosphate ceramics. This result implicates that the bone-like apatite may play an important role in the osteoinduction of Ca-P materials. The dynamic model used in this study may be better than usually used static immersion model in imitating the physiological condition of bone-like apatite formation. Dynamic SBF method is very useful to understand bone-like apatite formation in vivo and the mechanism of ectopic bone formation in calcium phosphate ceramics.
authors.affiliation: State key Laboratory for Modification of Chemical Fibers and Polymer Material, Donghua University, Shanghai, 200051, People's Republic of China. yrduan@dhu.edu.cn
authors.forename: Y R
authors.initials: YR
authors.lastname: Duan
authors.forename: Z R
authors.initials: ZR
authors.lastname: Zhang
authors.forename: C Y
authors.initials: CY
authors.lastname: Wang
authors.forename: J Y
authors.initials: JY
authors.lastname: Chen
authors.forename: X D
authors.initials: XD
authors.lastname: Zhang
mesh_terms: D001031:Apatites / Q000737:chemistry*; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry*; D001846:Bone Development; D002130:Calcium Phosphates / Q000032:analysis / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods*; D044085:Microfluidics / Q000379:methods; D008855:Microscopy, Electron, Scanning; D018482:Muscle, Skeletal; D013499:Surface Properties; D013997:Time Factors
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D002130:Calcium Phosphates