Comparative analysis of a BAC contig of porcine chromosome 13q31-q32 and human chromosome 3q21-q22.
BACKGROUND
The gene(s) encoding the ETEC F4ab/ac receptors, involved in neonatal diarrhoea in pigs (a disease not yet described in humans), is located close to the TF locus on Sscr13. In order to reveal and characterize possible candidate genes encoding these receptors, a porcine physical map of the TF region is indispensable.


RESULTS
A contig of 33 BAC clones, covering approximately 1.35 Mb surrounding the TF locus on Sscr13q31-q32, was built by chromosome walking. A total of 22,552 bp from the BAC contig were sequenced and compared with database sequences to identify genes, ESTs and repeat sequences, and to anchor the contig to the syntenic region of the human genome sequence (Hsap3q21-q22). The contig was further annotated based on this human/porcine comparative map, and was also anchored to the Sanger porcine framework map and the integrated map of Sscr13 by RH mapping.


CONCLUSIONS
The annotated contig, containing 10 genes and 2 ESTs, showed a complete conservation of linkage (gene order and orientation) with the human genome sequence, based on 46 anchor points. This underlines the importance of the human/porcine comparative map for the identification of porcine genes associated with genetic defects and economically important traits, and for assembly of the porcine genome sequence.
Van Poucke
Mario
M
Department of Animal Genetics and Breeding, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, B-9820 Merelbeke, Belgium. Mario.VanPoucke@UGent.be
Bourry
David
D
Piumi
François
F
Mattheeuws
Marc
M
Van Zeveren
Alex
A
Chardon
Patrick
P
Peelman
Luc J
LJ
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D016386:Chromosome Walking; D022202:Chromosomes, Artificial, Bacterial; D002893:Chromosomes, Human, Pair 3; D020451:Contig Mapping / Q000379:methods*; D017931:DNA Primers / Q000737:chemistry; D020224:Expressed Sequence Tags; D008040:Genetic Linkage; D015894:Genome, Human; D006801:Humans; D009693:Nucleic Acid Hybridization; D020161:Physical Chromosome Mapping; D021101:Radiation Hybrid Mapping; D013045:Species Specificity; D013552:Swine
D017931:DNA Primers