title: "Green" route to 6-hydroxy-3-succinoyl-pyridine from (S)-nicotine of tobacco waste by whole cells of a Pseudomonas sp.
abstract: A new technology for 6-hydroxy-3-succinoyl-pyridine (HSP) production from (S)-nicotine in tobacco waste by whole cells of a Pseudomonas sp. has been developed. When deionized water was used in the transformation reaction as a medium and the initial pH value of reaction mixture was adjusted to 7.0, 1.45 g/L HSP was produced from 3 g/L of nicotine in 5 h with 3.4 g/L of cells in a 5-L flask at 30 degrees C. HSP could be easily purified from the reaction without perplexing separation steps. A quantity of 1.3 g of HSP was recovered without impurity, and the overall yield of HSP was 43.8% (w/w), based on an initial concentration of 3.0 g/L of nicotine in reaction. This biotransformation made it possible to convert nicotine in tobacco wastes with high nicotine content into valuable compounds.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Shu Ning
authors.initials: SN
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.forename: Hong Zhi
authors.initials: HZ
authors.lastname: Tang
authors.forename: Jing
authors.initials: J
authors.lastname: Meng
authors.forename: Xiao Lei
authors.initials: XL
authors.lastname: Liu
authors.forename: Cui
authors.initials: C
authors.lastname: Qing
mesh_terms: D001711:Biotransformation; D002855:Chromatography, Thin Layer; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D009538:Nicotine / Q000737:chemistry / Q000378:metabolism*; D011549:Pseudomonas / Q000166:cytology / Q000378:metabolism*; D011725:Pyridines / Q000737:chemistry / Q000378:metabolism*; D013386:Succinates / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana / Q000737:chemistry*; D014028:Tobacco Smoke Pollution / Q000517:prevention & control*; D014867:Water / Q000737:chemistry
chemical_list: C507268:6-hydroxy-3-succinoyl-pyridine; D007220:Industrial Waste; D011725:Pyridines; D013386:Succinates; D014028:Tobacco Smoke Pollution; D014867:Water; D009538:Nicotine