title: [Screening and identification of phage-displayed polypeptides specifically binding to human gastric cancer with high metastatic potential to peritoneum].
abstract: OBJECTIVE
  By means of phage-display technique, to screen polypeptides that specifically bind to human gastric cancer with high metastatic potential to peritoneum.
  
  
  METHODS
  Two human gastric cancer cell lines were used: GC9811-P with high metastatic potential to peritoneum and its wild type parental GC9811, to carry out subtractive screening with a phage display-12 peptide library.
  
  
  RESULTS
  After three rounds of screening, 40 phage clones bond to GC9811-P cells were randomly selected. When injected into the peritoneal cavity of nude mice, 6 of the 40 clones did not bind to mouse peritoneum as examined by immunohistochemical staining. They were considered to be capable of binding specifically to GC9811-P cells. Sequence analysis revealed two different exogenous peptides: TLNINRLILPRT and SMSI(X)SPYI(XXX).
  
  
  CONCLUSIONS
  Two peptides have been obtained that specifically bind to a gastric cancer cell variant GC9811-P, which easily disseminates to the peritoneum. Whether or not they could block GC9811-P metastasis to peritoneum in vivo remains to be determined.
authors.affiliation: Affiliated Hospital, Ningxia Medical College, Yinchuan 750004, China.
authors.forename: Ke-dong
authors.initials: KD
authors.lastname: Zhang
authors.forename: Xin-ning
authors.initials: XN
authors.lastname: Guo
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Dong-tao
authors.initials: DT
authors.lastname: Zhang
authors.forename: Fei-hu
authors.initials: FH
authors.lastname: Bai
authors.forename: Hai-ping
authors.initials: HP
authors.lastname: Jiang
authors.forename: Hui-hong
authors.initials: HH
authors.lastname: Zhai
authors.forename: Yong-zhan
authors.initials: YZ
authors.lastname: Nie
authors.forename: Kai-chun
authors.initials: KC
authors.lastname: Wu
authors.forename: Dai-ming
authors.initials: DM
authors.lastname: Fan
mesh_terms: D000818:Animals; D001665:Binding Sites; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D010534:Peritoneal Neoplasms / Q000556:secondary*; D040081:Protein Array Analysis / Q000379:methods; D011485:Protein Binding; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D019151:Peptide Library; D010455:Peptides