title: Summary of the animal homologue section of HLDA8.
abstract: Development of reagents against leukocyte differentiation antigens in veterinary immunology is slower compared to humans and mice. Cross-reactivity studies with monoclonal antibodies (mAb) generated against human molecules represent an excellent approach for the detection of new reagents for the minor characterised species. Three hundred seventy-seven commercially available mAb from different companies were tested for their reactivity with cells from 17 species--including non-human primates, ruminants, swine, horse, carnivores, rabbit, guinea pig, chicken and fish. In a first round of testing by flow cytometry (FCM) 182 mAb showed reactivity with atleast one of the species described above. Most of the cross-reactivity was found against non-human primate leukocytes, but also species in evolutionarily more distant from humans showed in some cases a clear staining pattern in flow cytometry (FCM). In a second round these FCM-results were confirmed by molecular analyses, by immunoprecipitation studies and analyses on transfectants. Interesting was the broad species-overlapping reactivity of mAb directed against CD9 (11 out of 17 species), CD11a (11/17), CD14 (11/17), CD18 (13/17), CD21 (7/17), CD29 (10/17), CD44 (13/17), CD45 (9/17), CD47 (10/17), and CD49d (13/17), CD61 (6/17), CD86 (7/17), CD91 (5/17), and CD172a (10/17), indicating evolutionary highly conserved epitopes on these surface molecules. Our results suggest the suitability of crossreactive mAb for the animal model studies. Moreover, these findings contribute to our understanding of the evolution of the immune system.
authors.affiliation: Clinical Immunology, Veterinary University of Vienna, Vienna, Austria. armin.saalmueller@vu-wien.ac.at
authors.forename: Armin
authors.initials: A
authors.lastname: Saalmüller
authors.forename: Joan K
authors.initials: JK
authors.lastname: Lunney
authors.forename: Claudia
authors.initials: C
authors.lastname: Daubenberger
authors.forename: William
authors.initials: W
authors.lastname: Davis
authors.forename: Uwe
authors.initials: U
authors.lastname: Fischer
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Göbel
authors.forename: Phil
authors.initials: P
authors.lastname: Griebel
authors.forename: Enoc
authors.initials: E
authors.lastname: Hollemweguer
authors.forename: Todd
authors.initials: T
authors.lastname: Lasco
authors.forename: Richard
authors.initials: R
authors.lastname: Meister
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Schuberth
authors.forename: Karol
authors.initials: K
authors.lastname: Sestak
authors.forename: Paul
authors.initials: P
authors.lastname: Sopp
authors.forename: Falko
authors.initials: F
authors.lastname: Steinbach
authors.forename: Wu
authors.initials: W
authors.lastname: Xiao-Wei
authors.forename: Bent
authors.initials: B
authors.lastname: Aasted
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D002417:Cattle; D002645:Chickens; D003429:Cross Reactions; D005399:Fishes; D006168:Guinea Pigs; D006736:Horses; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D011323:Primates; D011817:Rabbits; D012418:Ruminants; D012756:Sheep; D013045:Species Specificity; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD