[Dynamic expression of VIPR2 in form deprivation myopia].
OBJECTIVE
To investigate the dynamic expression and significance of vasoactive intestinal peptide receptor 2 (VIPR2) on retina-choroid-clera in high myopia.


METHODS
Twenty-one yellow chicks of 1 day old were used in the research. The right eyes were the experimental group, covered continuously for 1 week, 2 weeks and 4 weeks respectively. The left eyes were not covered as the normal control group. Both groups were detected diopter degrees using retinoscopic refraction, determinated eyeball axis using ophthalmology ultra-A, and investigated VIPR2 expression on retina-choroid-sclera in both groups at three stages by SP immunohistochemical staining.


RESULTS
The experimental eyes changed from hypermetropia at pre-experiment to high myopia during the experiment stages, and the diopter degrees were deeper and eyeball axis was longer along with the period of being covered. Both groups had strong expression of VIPR2 on photoreceptor-outer segment of the retina and choroids. The expression was down-regulated with the time in both groups. Compared with the control group, VIPR2 expression of the experimental group was significantly up-regulated (P < 0.05).


CONCLUSIONS
Form deprivation could induce high myopia. The expression of VIPR2 existed on photoreceptor-outer segment of the retina and choroids. VIPR2 may play an important role on the formation and development of myopia.
Liu
Shuang-zhen
SZ
Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha 410008, China. liushzhxy@163.com
Wang
Hua
H
Jiang
Jing-jing
JJ
Wang
Ping-bao
PB
Wu
Xiao-ying
XY
Tan
Xing-ping
XP
Xia
Zhao-hua
ZH
D000818:Animals; D000831:Animals, Newborn; D002645:Chickens; D002829:Choroid / Q000378:metabolism*; D005260:Female; D008297:Male; D009216:Myopia / Q000209:etiology / Q000378:metabolism*; D051239:Receptors, Vasoactive Intestinal Peptide, Type II / Q000096:biosynthesis* / Q000235:genetics; D012160:Retina / Q000378:metabolism*
D051239:Receptors, Vasoactive Intestinal Peptide, Type II