Protein profiles of bovine placenta derived from somatic cell nuclear transfer.
Practical application of animal cloning by somatic cell nuclear transfer (SCNT) has been hampered by an extremely low success rate. To address whether placental dysfunction in SCNT causes fetal loss during pregnancy, we have used a global proteomics approach using 2-DE and MS to analyze the differential protein patterns of three placentae from the afterbirth of cases of postnatal death, derived from SCNT of Korean Native cattle, and three normal placentae obtained from the afterbirth of fetuses derived from artificial insemination. Proteins within a pI range of 4.0-7.0 and 6.0-9.0 were analyzed separately by 2-DE in triplicate. A total of approximately 2000 spots were detected in placental 2-DE gels stained with CBB. In the comparison of normal and SCNT samples, 60 spots were identified as differentially expressed proteins, of which 33 spots were up-regulated proteins in SCNT placentae, while 27 spots were down-regulated proteins. Most of the proteins identified in this analysis appeared to be related with protein repair or protection, cytoskeleton, signal transduction, immune system, metabolism, extracellular matrix and remodeling, transcription regulation, cell structure or differentiation and ion transport. One of up-regulated proteins in SCNT was TIMP-2 protein known to be related to extracellular matrix and remodeling during pregnancy. Western blot analysis showed an increased level of TIMP-2 in SCNT placenta compared to normal. Our results revealed composite profiles of key proteins involved in abnormal placenta derived from SCNT, and suggested expression abnormality of these genes in SCNT placenta, resulting in fetal losses following SCNT.
Kim
Hong Rye
HR
Research Center for Transgenic Cloned Pigs, Chungnam National University, Yuseong-Gu, Daejeon, Korea.
Kang
Jae Ku
JK
Yoon
Jong Taek
JT
Seong
Hwan Hoo
HH
Jung
Jin Kwan
JK
Lee
Hong Mie
HM
Sik Park
Chang
C
Jin
Dong Il
DI
D000818:Animals; D030801:Animals, Genetically Modified; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D007315:Insemination, Artificial; D053652:Nuclear Transfer Techniques; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism
D011257:Pregnancy Proteins; D019716:Tissue Inhibitor of Metalloproteinase-2