Real-time measurement of solute partitioning to lipid monolayers.
The interaction of a peripheral protein with a lipid-water interface can show a pronounced dependence on the composition and two-dimensional packing density of the lipids that comprise the interface. We report a novel optical method for measuring the adsorption of macromolecules, such as proteins and nucleic acids, and smaller solutes, such as drugs, to lipid monolayers at the gas-liquid interface. Using fluorescence emission from proteins and a small molecule, we demonstrate that the emissions from these solutes when in the aqueous phase and when associated with the monolayer can be temporally separated. Such separation allows measurement of the extent of solute adsorption, spectral characterization of the adsorbed solute, and characterization of lipid organization using adsorption kinetics. The method does not require, but is compatible with, the solute having different spectral properties in the bulk and surface phases. Indeed, if optical signals from adsorbed and soluble solute are the same or their relationship is known, absolute surface excess of adsorbed solute can be calculated without independent calibration. With appropriate instrumental configuration, the method should be adaptable for screening solutes for interaction with planar monolayers having both well-defined composition and adjustable lipid packing density.
Momsen
W E
WE
The Hormel Institute, University of Minnesota, Austin, MN 55912, USA.
Mizuno
N K
NK
Lowe
M E
ME
Brockman
H L
HL
D000327:Adsorption; D001896:Boron Compounds; D003089:Colipases / Q000737:chemistry; D005453:Fluorescence; D007700:Kinetics; D008049:Lipase / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D003089:Colipases; D008055:Lipids; D011506:Proteins; D008049:Lipase