Effect of E(sev) and Su(Raf) Hsp83 mutants and trans-heterozygotes on bristle trait means and variation in Drosophila melanogaster.
The Hsp90 protein encoded by the Hsp83 gene is required for the development of many traits in Drosophila. Hsp83 is also thought to play a role in the expression of phenotypic and genetic variability for subsequent selection and evolutionary change. Here we examine the impact of different E(sev) and Su(Raf) Hsp83 mutants on means and phenotypic variances of invariant and variable bristle traits. One of the mutants influenced the normally invariant thoracic bristle number, while none affected invariant scutellar bristle number. E(sev) alleles consistently influenced variable bristle traits while there were fewer effects of the Su(Raf) alleles. For the variable traits, none of the Hsp83 alleles had any effect on phenotypic variance, environmental variance, or developmental stability of the bristle traits. When alleles were combined in trans-heterozygotes, there were both cumulative and complementary effects on thoracic and variable bristle trait numbers, depending on the allelic combination. Overall, the results suggest that Hsp83 mutants do not have detectable effects on the phenotypic or environmental variance of bristle traits and that complementation of E(sev) and Su(Raf) Hsp83 mutants can extend to thoracic bristles as well as previously reported effects on viability. Some allelic combinations lead to more severe effects on variable bristle trait means than do single Hsp83 mutations.
Milton
Claire C
CC
Centre for Environmental Stress and Adaptation Research, Department of Genetics, University of Melbourne, Melbourne, Victoria 3010, Australia. claire.milton@ucl.ac.uk
Batterham
Philip
P
McKenzie
John A
JA
Hoffmann
Ary A
AA
D000483:Alleles; D000704:Analysis of Variance; D000818:Animals; D003433:Crosses, Genetic; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006360:Heat-Shock Proteins / Q000235:genetics*; D006579:Heterozygote; D008297:Male; D009154:Mutation; D010641:Phenotype; D019655:Quantitative Trait, Heritable
D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; C089272:Hsp83 protein, Drosophila