title: An image contrast agent selectively activated by prostate specific antigen.
abstract: A family of image contrast agent conjugates designed to undergo enzymatic activation has been synthesized. The agents underwent activation both with enzymatically active prostate specific antigen (PSA) and alpha-chymotrypsin, releasing free fluorophore via cleavage of a three-component system. A hexapeptide derivative showed exclusive activation by PSA and constitutes a method for tracking PSA activity in vitro.
authors.affiliation: Bioorganic and Medicinal Chemistry Laboratories, Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA. gr.jones@neu.edu
authors.forename: Graham B
authors.initials: GB
authors.lastname: Jones
authors.forename: Curtis F
authors.initials: CF
authors.lastname: Crasto
authors.forename: Jude E
authors.initials: JE
authors.lastname: Mathews
authors.forename: Longfei
authors.initials: L
authors.lastname: Xie
authors.forename: Miguel O
authors.initials: MO
authors.lastname: Mitchell
authors.forename: Ahmed
authors.initials: A
authors.lastname: El-Shafey
authors.forename: Anthony V
authors.initials: AV
authors.lastname: D'Amico
authors.forename: Glenn J
authors.initials: GJ
authors.lastname: Bubley
mesh_terms: D002918:Chymotrypsin / Q000737:chemistry; D003287:Contrast Media / Q000737:chemistry*; D004789:Enzyme Activation; D009682:Magnetic Resonance Spectroscopy; D017430:Prostate-Specific Antigen / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D003287:Contrast Media; D002918:Chymotrypsin; D017430:Prostate-Specific Antigen