Cloning and sequencing of 28 kDa outer membrane protein gene of Brucella melitensis Rev. 1.
Brucella melitensis is an organism of paramount zoonotic importance. The 28 kDa outer membrane protein (OMP) is one of the immunodominant antigens of B. melitensis. The gene encoding 28 kDa OMP (omp28) has been amplified from B. melitensis Rev. 1 strain. A PCR product of 753 bp, encoding complete omp28 gene of B. melitensis, was obtained. The gene was further cloned and sequenced. The nucleotide sequence of B. melitensis Rev. 1 strain showed substitution of 2 nucleotides from that of 16M strain.
Chaudhuri
Pallab
P
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India. pallab@ivri.up.nic.in
Kumar
S Vinoth
SV
Prasad
Rajeev
R
Srivastava
S K
SK
Yadav
M P
MP
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001483:Base Sequence; D002004:Brucella Vaccine / Q000378:metabolism; D017347:Brucella melitensis / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002004:Brucella Vaccine; D011994:Recombinant Proteins