title: Antioxidant phenylacetic acid derivatives from the seeds of Ilex aquifolium.
abstract: Reversed-phase preparative HPLC analysis of the methanol extract of the seeds of Ilex aquifolium afforded two antioxidant phenylacetic acid derivatives, 2,4-dihydroxyphenylacetic acid (1) and 2,4-dihydroxyphenylacetic acid methyl ester (2). The structures were determined by spectroscopic methods. In the DPPH assay for antioxidant activity, the IC(50) values of 1 and 2 were 1.50 x 10(-3) and 2.55 x 10(-3) mg mL(-1), respectively, compared to 2.88 x 10(-5) mg mL(-1) of quercetin, a natural antioxidant.
authors.affiliation: School of Life Sciences, The Robert Gordon University, Aberdeen AB25 1HG, Scotland, UK.
authors.forename: Lutfun
authors.initials: L
authors.lastname: Nahar
authors.forename: Wendy R
authors.initials: WR
authors.lastname: Russell
authors.forename: Moira
authors.initials: M
authors.lastname: Middleton
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shoeb
authors.forename: Satyajit D
authors.initials: SD
authors.lastname: Sarker
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006834:Hydrazines / Q000737:chemistry; D030017:Ilex / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010648:Phenylacetates / Q000737:chemistry* / Q000302:isolation & purification; D010851:Picrates; D012639:Seeds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D006834:Hydrazines; D010648:Phenylacetates; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl