title: [Influence of regenerated fluid in vitreous cavity at various periods after vitreoretinal microsurgery on the proliferation and bFGF secretion of cultured human retinal pigment epithelial cells].
abstract: OBJECTIVE
  To demonstrate the mechanism of positive effects of the sequential air-fluid exchange on the use of complicated vitroretinal microsurgery.
  
  
  METHODS
  Seventy-one patients who underwent vitreoretinal microsurgery were performed 2 or 3 times sequential air-fluid exchange. The regenerated fliud in vitreous cavity at various periods was collected to act on cultured human retinal pigment epithelial (RPE) cells,and then the secretion of bFGF and the expressions of bcl-2 and ki-67 by RPE cells were observed.
  
  
  RESULTS
  The expressions of bcl-2 and ki-67 were up-regulated and the secretion of bFGF significantly increased after RPE cells was acted with the regenerated fluid in the vitreous cavity.
  
  
  CONCLUSIONS
  The sequential air-fluid exchange can mechanically reduce intraocular growth factors after the vitreoretinal microsurgery, indirectly restrain the proliferation of RPE cell, and improve the successful rate of vitreoretinal microsurgery.
authors.affiliation: Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha 410011, China. zhqh1975@yahoo.com.cn
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Zhou
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Zhu
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis / Q000235:genetics; D008866:Microsurgery; D010857:Pigment Epithelium of Eye / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D012038:Regeneration; D012160:Retina / Q000601:surgery; D012163:Retinal Detachment / Q000517:prevention & control; D055502:Secondary Prevention; D014821:Vitrectomy; D018630:Vitreoretinopathy, Proliferative / Q000601:surgery*
chemical_list: D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D016222:Fibroblast Growth Factor 2