Induction of stable RNA interference in mammalian cells.
Over the last years, RNA interference (RNAi) has become a widely used technique that permits the knock-down, and hence functional analysis, of individual genes in vertebrate cells. However, the high failure rate of the RNA molecules used in RNAi experiments continues to be a problem. In this paper, I describe a set of design criteria, experimental steps and expression vectors that can facilitate the effective knock-down of almost any vertebrate gene product in cultured cells or in experimental animals.Gene Therapy (2006) 13, 503-508. doi:10.1038/sj.gt.3302656; published online 29 September 2005.
Cullen
Bryan R
BR
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA. culle002@mc.duke.edu
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D008322:Mammals; D035683:MicroRNAs / Q000008:administration & dosage*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage
D035683:MicroRNAs; D034741:RNA, Small Interfering