title: Genetic toxoids of Shiga toxin types 1 and 2 protect mice against homologous but not heterologous toxin challenge.
abstract: Shiga toxin type 1 (Stx1) and type 2 (Stx2) are produced by Escherichia coli O157:H7 and are responsible for the life-threatening sequela, the hemolytic uremic syndrome. Whether antisera to Stx1 or Stx2 are cross-neutralizing remains controversial, so we constructed genetic toxoids of Stx1 and Stx2 and evaluated them as vaccines. Antisera from mice immunized with a single toxoid type recognized and neutralized the homologous toxin but not the heterologous toxin. Furthermore, only mice immunized with Stx1 and Stx2 toxoids were protected against a lethal challenge of both toxins. We conclude that Stx1 and Stx2 are distinct antigens for mice.
authors.affiliation: Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, B4052, 4301 Jones Bridge Road, Bethesda, MD 20814-4799, USA.
authors.forename: Sharon X
authors.initials: SX
authors.lastname: Wen
authors.forename: Louise D
authors.initials: LD
authors.lastname: Teel
authors.forename: Nicole A
authors.initials: NA
authors.lastname: Judge
authors.forename: Alison D
authors.initials: AD
authors.lastname: O'Brien
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D005260:Female; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D022622:Shiga Toxin 1 / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D022641:Shiga Toxin 2 / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D014121:Toxoids / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D014121:Toxoids