Gene expression profiles in response to Fas stimulation in Trypanosoma cruzi-infected host cells.
To determine the molecular mechanism by which apoptosis is inhibited in Trypanosoma cruzi-infected host cells, we used human cDNA apoptosis chips to compare the gene expression profiles in response with 'death ligands target' (Fas) stimulation in infected and uninfected cells. Of the 164 apoptosis-related genes examined, 20, including those encoding both pro- and anti-apoptotic proteins, were highly up-regulated in the infected group. Genes encoding caspases and apoptosis inhibitors were optimally expressed 10-30 min after induction of apoptosis, whereas genes involved in transcriptional regulation and cell proliferation were up-regulated after 2-24 h. These results suggest that host anti-apoptotic gene(s) may play a crucial role in the inhibition of Fas-mediated apoptosis in T. cruzi-infected cells.
Hashimoto
Muneaki
M
Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, 2-1-1, Hongo, Tokyo 113-8421, Japan.
Nakajima-Shimada
Junko
J
Ishidoh
Kazumi
K
Aoki
Takashi
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D017209:Apoptosis / Q000235:genetics; D015152:Blotting, Northern; D020169:Caspases / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D014355:Chagas Disease / Q000235:genetics / Q000469:parasitology* / Q000473:pathology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D013997:Time Factors; D014158:Transcription, Genetic / Q000235:genetics; D019014:fas Receptor / Q000276:immunology*
D000911:Antibodies, Monoclonal; D019014:fas Receptor; D020169:Caspases