Negative association between serum dioxin level and oxidative DNA damage markers in municipal waste incinerator workers.
OBJECTIVE
To investigate the effect of dioxin on the formation of oxidative DNA damage and urinary mutagenicity, we measured the concentrations of serum dioxins and lymphocytic 8-hydroxydeoxyguanosine (8-OH-dG) in 57 male waste incinerator workers, urinary 8-OH-dG and urinary mutagenicity in 29 male waste incinerator workers.


METHODS
Information about the subjects was obtained from a questionnaire. Concentrations of polychlorinated dibenzo-p-dioxin (PCDD), polychlorinated dibenzofuran (PCDF), and coplanar-polychlorinated-biphenyl (Co-PCB) in serum samples from the workers were measured with a high-resolution gas chromatograph /high-resolution mass spectrometer. Lymphocytic and urinary 8-OH-dG levels were measured with a high-performance liquid chromatography-electrochemical detector system. The urinary mutagenicity was measured with umu assay.


RESULTS
The lymphocytic 8-OH-dG level showed a negative association with the serum dioxin level (total value of TEQ-PCDD, PCDF, and Co-PCB). Urinary 8-OH-dG did not show correlation with serum dioxin level, but showed positive correlation with the smoking index.


CONCLUSIONS
With respect to the subjects' serum dioxin level, dioxin did not increase the urinary 8-OH-dG level by oxidative DNA damage, but upregulation of the primary defenses with oxidative damage and/or DNA repair system activity might have occurred.
Yoshida
Jin
J
Department of Environmental Health, Osaka Prefectural Institute of Public Health, Osaka, 1-3-69, Nakamichi, Higashinari-ku, 537-0025, Japan. jnyosida@iph.pref.osaka.jp
Kumagai
Shinji
S
Tabuchi
Takeo
T
Kosaka
Hiroshi
H
Akasaka
Susumu
S
Kasai
Hiroshi
H
Oda
Hajime
H
D000328:Adult; D004249:DNA Damage / Q000235:genetics*; D004147:Dioxins / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D005819:Genetic Markers; D006801:Humans; D017745:Incineration; D007564:Japan; D008297:Male; D008875:Middle Aged; D009152:Mutagenicity Tests; D016273:Occupational Exposure; D018384:Oxidative Stress; D012037:Refuse Disposal
D004147:Dioxins; D005819:Genetic Markers