title: Elicitation of defense related enzymes and resistance by L-methionine in pearl millet against downy mildew disease caused by Sclerospora graminicola.
abstract: Induction of resistance to downy mildew in pearl millet was studied after treatment with L-methionine. The disease severity was drastically reduced in the seedlings after treatment with L-methionine. Northern hybridization was used to study the transcript (mRNA) accumulation of defense response genes upon treatment with L-methionine in pearl millet seedlings and the mechanism during the induction of resistance. The gene activation patterns in the highly resistant cultivar IP18294 were compared with those exhibited by the susceptible cultivar 7042S at various time intervals to determine if differences in timing or levels of transcript accumulation could be correlated with the differences in the susceptibility of pearl millet to the downy mildew pathogen, Sclerospora graminicola. The mRNA levels of genes for Pr-1a, beta-1,3-glucanase, chitinase, peroxidase, lipoxygenases and chalcone synthase showed an increase after inoculation with S. graminicola. Significant gene activation was observed in the susceptible cultivar 7042S after treatment with L-methionine followed by challenge inoculation with the downy mildew pathogen which was comparable to the resistant cultivar.
authors.affiliation: Department of Studies in Applied Botany and Biotechnology, University of Mysore, Manasagangotri, Mysore 570 006, India.
authors.forename: Bejai R
authors.initials: BR
authors.lastname: Sarosh
authors.forename: Subramoniam
authors.initials: S
authors.lastname: Sivaramakrishnan
authors.forename: Hunthrike S
authors.initials: HS
authors.lastname: Shetty
mesh_terms: D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D006026:Glycoside Hydrolases / Q000378:metabolism; D008715:Methionine / Q000494:pharmacology*; D009868:Oomycetes / Q000502:physiology*; D031782:Pennisetum / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology; D015533:Transcriptional Activation
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D008715:Methionine; D006026:Glycoside Hydrolases