title: Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima.
abstract: Amphibian skin is a rich resource of bioactive peptides like proline-rich bombesin from frog Bombina maxima. A novel cDNA clone encoding a precursor protein that comprises proline-rich bombesin and a novel peptide, designated as bombestatin, was isolated from a skin cDNA library of B. maxima. The predicted primary structure of the novel peptide is WEVLLNVALIRLELLSCRSSKDQDQKESCGMHSW, in which two cysteines form a disulfide bond. A BLAST search of databases did not detect sequences with significant similarity. Bombestatin possesses dose-dependent contractile activity on rat stomach strips. The differences between cDNAs encoding PR-bombesin plus bombestatin and PR-bombesin alone are due to fragment insertions located in 3'-coding region and 3'-untranslational region, respectively.
authors.affiliation: Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Shen
authors.forename: Shu-Bai
authors.initials: SB
authors.lastname: Liu
authors.forename: Ying-Xia
authors.initials: YX
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Jin
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Lee
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001001:Anura / Q000235:genetics*; D001483:Base Sequence; D001839:Bombesin / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D015723:Gene Library; D008297:Male; D008969:Molecular Sequence Data; D011498:Protein Precursors; D012867:Skin / Q000737:chemistry*
chemical_list: D029845:Amphibian Proteins; D018076:DNA, Complementary; D011498:Protein Precursors; C510988:bombestatin, Bombina maxima; D001839:Bombesin