Association of ribosomal proteins with nascent transcripts in S. cerevisiae.
Although it is generally accepted that transcription and translation are spatially separated in eukaryotes, a number of recent observations have called this belief into question. In particular, several studies have shown that parts of the translation machinery, including ribosomal proteins, can be found associated with sites of active transcription in metazoans. Here we describe results of chromatin immunoprecipitation (ChIP) experiments designed to determine whether ribosomal proteins associate with nascent transcripts in Saccharomyces cerevisiae and whether this association reflects a functional engagement of the translation machinery. We find that HAT-tagged ribosomal proteins can be detected in association with nascent RNAs in budding yeast. However, our data clearly indicate that this binding is independent of transcript translatability, so is therefore not indicative of nuclear translation.
Schroder
Patricia A
PA
Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, MA 02454-9110, USA.
Moore
Melissa J
MJ
D015153:Blotting, Western; D002499:Centrifugation, Density Gradient; D047369:Chromatin Immunoprecipitation; D005800:Genes, Fungal; D006238:Haploidy; D010957:Plasmids; D014176:Protein Biosynthesis; D012331:RNA, Fungal / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D006163:Ribonuclease T1 / Q000494:pharmacology; D012259:Ribonuclease, Pancreatic / Q000494:pharmacology; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic