Possible role of sildenafil in inhibiting rat vas deferens contractions by influencing the purinergic system.
OBJECTIVE
To evaluate the effect of sildenafil, a selective inhibitor of cyclic guanosine monophosphate (cGMP)-selective type 5 phosphodiesterase, on isolated rat vas deferens and its connections with the purinergic system.


METHODS
Epididymal and prostatic portions of isolated vas deferens were placed in organ baths containing Krebs' solution. Contractions were induced by noradrenaline (NA), adenosine triphosphate (ATP), alpha,beta-methylene ATP and electrical field stimulation (EFS). The effect of sildenafil on the contractions was compared with suramin and Evans blue (EB).


RESULTS
NA, ATP, alpha,beta-methylene ATP and EFS caused contractions in both portions of vas deferens. NA-induced contractions were unaffected by sildenafil and suramin but potentiated by EB. ATP-induced contractions were non-competitively inhibited in both portions by sildenafil and suramin but potentiated by EB. alpha,beta-methylene ATP-induced contractions were unaffected by sildenafil but were inhibited in both portions by suramin and EB. EFS-induced contractions were inhibited by sildenafil and suramin while potentiated by EB.


CONCLUSIONS
Sildenafil inhibited the contractions in both portions of vas deferens, as did suramin. We have suggested that purinergic system has a role in this antagonism and it seems to be mediated by an ATP-dependent mechanism instead of a receptor interaction.
Bilge
S Sirri
SS
University of Ondokuz Mayis, Department of Pharmacology, Kurupelit, Samsun, Turkey. ssbilge@omu.edu.tr
Kesim
Yuksel
Y
Kurt
Mehmet
M
Aksoz
Elif
E
Celik
Suleyman
S
D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D011687:Purines; D051381:Rats; D017208:Rats, Wistar; D011983:Receptors, Purinergic / Q000187:drug effects*; D000068677:Sildenafil Citrate; D013450:Sulfones; D014649:Vas Deferens / Q000187:drug effects* / Q000502:physiology*
D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D011983:Receptors, Purinergic; D013450:Sulfones; D000068677:Sildenafil Citrate