title: Gene expression profiles of intact and regenerating zebrafish retina.
abstract: OBJECTIVE
  Investigate the molecular determinants of retinal regeneration in adult vertebrates by analyzing the gene expression of control and post-lesion retina of adult zebrafish, a system that regenerates following injury.
  
  
  METHODS
  Gene expression of zebrafish retina and brain were determined with DNA microarray, RT-PCR, and real-time quantitative PCR analyses. Damaged retinas and their corresponding controls were analyzed 2-5 days post-lesion (acute injury condition) or 14 d post-lesion (cell regeneration condition).
  
  
  RESULTS
  Expected similarities and differences in the gene expression profile of zebrafish retina and brain were observed, confirming the applicability of the gene expression techniques. Mechanical lesion of retina triggered significant, time-dependent changes in retinal gene expression. The induced transcriptional changes were consistent with cellular phenomena known to occur, in a time-dependent manner, subsequent to retinal lesion, including cell cycle progression, axonal regeneration, and regenerative cytogenesis.
  
  
  CONCLUSIONS
  The results indicate that retinal regeneration in adult zebrafish involves a complex set of induced, targeted changes in gene transcription, and suggest that these molecular changes underlie the ability of the adult vertebrate retina to regenerate.
authors.affiliation: Department of Neuroscience and Physiology, SUNY Upstate Medical University, Syracuse, NY 13210, USA. camerond@upstate.edu
authors.forename: David A
authors.initials: DA
authors.lastname: Cameron
authors.forename: Karen L
authors.initials: KL
authors.lastname: Gentile
authors.forename: Frank A
authors.initials: FA
authors.lastname: Middleton
authors.forename: Patrick
authors.initials: P
authors.lastname: Yurco
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D012160:Retina / Q000293:injuries / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014945:Wound Healing / Q000502:physiology*; D015027:Zebrafish / Q000502:physiology*
chemical_list: D017931:DNA Primers