title: Diagnostic assays applied in BVDV control in The Netherlands.
abstract: In The Netherlands, a voluntary bovine virus diarrhoea virus (BVDV)-free certification programme was started in 1997. After an intake procedure in which all cattle are tested for the presence of BVD virus, a herd obtains the status "BVD-virus-free". To maintain this status a monitoring procedure is executed to verify absence of BVDV circulation in the herd. Several diagnostic tests are used: RT-PCR in bulk milk and pooled blood samples, antigen-ELISA (Ag-ELISA) and antibody ELISA in individual blood samples. Sensitivity and specificity of these tests are discussed. In addition, a diagnostic quick scan has been introduced, consisting of a combination of bulk milk tests for virus and antibody, and antibody tests in samples from young stock. Preliminary results are presented.
authors.affiliation: Animal Health Service, P.O. Box 9, 7400 AA Deventer, The Netherlands. m.mars@gdvdieren.nl
authors.forename: M H
authors.initials: MH
authors.lastname: Mars
authors.forename: C
authors.initials: C
authors.lastname: Van Maanen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis* / Q000517:prevention & control*; D002417:Cattle; D003955:Diagnostic Tests, Routine / Q000662:veterinary*; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D008892:Milk / Q000737:chemistry; D009426:Netherlands / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral