title: Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates.
abstract: Nuclear envelope (NE) formation during cell division in multicellular organisms is a central yet poorly understood biological process. We report that the conserved nucleoporin Nup155 has an essential function in NE formation in Caenorhabditis elegans embryos and in Xenopus laevis egg extracts. In vivo depletion of Nup155 led to failure of nuclear lamina formation and defects in chromosome segregation at anaphase. Nup155 depletion inhibited accumulation of nucleoporins at the nuclear periphery, including those recruited to chromatin early in NE formation. Electron microscopy analysis revealed that Nup155 is also required for the formation of a continuous nuclear membrane in vivo and in vitro. Time-course experiments indicated that Nup155 is recruited to chromatin at the time of NE sealing, suggesting that nuclear pore complex assembly has to progress to a relatively late stage before NE membrane assembly occurs.
authors.affiliation: European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Cerstin
authors.initials: C
authors.lastname: Franz
authors.forename: Peter
authors.initials: P
authors.lastname: Askjaer
authors.forename: Wolfram
authors.initials: W
authors.lastname: Antonin
authors.forename: Carmen López
authors.initials: CL
authors.lastname: Iglesias
authors.forename: Uta
authors.initials: U
authors.lastname: Haselmann
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Schelder
authors.forename: Ario
authors.initials: A
authors.lastname: de Marco
authors.forename: Matthias
authors.initials: M
authors.lastname: Wilm
authors.forename: Claude
authors.initials: C
authors.lastname: Antony
authors.forename: Iain W
authors.initials: IW
authors.lastname: Mattaj
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029742:Caenorhabditis elegans Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D020090:Chromosome Segregation / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism; D009685:Nuclear Envelope / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D034881:Nuclear Lamina / Q000235:genetics / Q000378:metabolism; D022022:Nuclear Pore / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D029867:Xenopus Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000235:genetics / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D002843:Chromatin; C506179:NUP155 protein, Xenopus; D028861:Nuclear Pore Complex Proteins; D029867:Xenopus Proteins; C506178:npp-8 protein, C elegans