[Determination of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside in the traditional Chinese preparation by high performance liquid chromatography with chemiluminescence detection].
Based on the chemiluminescence reaction of KMnO4-HNO3-2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside sensitized by HCHO, an analytical method for 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside by RP-HPLC with chemiluminescence detector was developed. The chromatographic conditions were optimized. AN ODS hypersil column(25 mm x 4.0 mm, 125 x 4.0 mm i.d. 5 microm) was used with acetonitrile--0.3% H3PO4 [25:75, (phi)] as mobile phase, and the flow rate was 0.8 mL x min(-1) at the column temperature of 40 degrees C. The linear range of calibration curve was from 15.75 to 136.5 microg x mL(-1), the detection limit was 11.83 microg x mL(-1) with a concentration of 21 microg x mL(-1) of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside, and the recovery was 102%-108%. The relative standard deviation was 3.45% (Cs = 21.0 microg x mL(-1), n = 3). The method has been applied to the determination of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside in a few Chinese medicines, and the results were satisfactory. The method was simple, rapid and highly selective.
Wei
Hong-qing
HQ
School of Chemistry and Life Sciences, Tianjin Normal University, Tianjin 300074, China.
Liu
Er-bao
EB
Ren
Rong-fang
RF
Zhao
Xiu-li
XL
Li
Xiao-xia
XX
Jiang
Feng-xian
FX
D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005557:Formaldehyde / Q000737:chemistry; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry; D007700:Kinetics; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D015394:Molecular Structure; D017942:Nitric Acid / Q000737:chemistry; D015203:Reproducibility of Results; D013267:Stilbenes / Q000032:analysis* / Q000737:chemistry; D013607:Tablets
D002214:Capsules; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D013267:Stilbenes; D013607:Tablets; C093026:2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside; D005557:Formaldehyde; D017942:Nitric Acid