Comparative evaluation of three different intramuscular delivery methods for DNA immunization in a nonhuman primate animal model.
Although plasmid DNA vaccines induce potent cell-mediated immune responses and prime for antibody responses in experimental laboratory animals, their immunogenicity in humans has been less remarkable. A number of strategies have been proposed to improve the immunogenicity of these vaccines, including using novel means of vaccine delivery. In the present study, the immunogenicity of three different methods of intramuscular plasmid DNA administration was compared in cynomolgus monkeys: needle and syringe, Biojector 2000, and Mini-Ject. The elicited cellular and humoral immune responses were comparable in monkeys immunized using these different delivery techniques, suggesting that the needle-free approaches to vaccine administration do not significantly improve the immunogenicity of the plasmid DNA vaccine used in the study.
Rao
Srinivas S
SS
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Gomez
Phillip
P
Mascola
John R
JR
Dang
Vi
V
Krivulka
Georgia R
GR
Yu
Faye
F
Lord
Carol I
CI
Shen
Ling
L
Bailer
Robert
R
Nabel
Gary J
GJ
Letvin
Norman L
NL
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000032:analysis / Q000096:biosynthesis; D015497:HIV-1 / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D007273:Injections, Intramuscular / Q000379:methods*; D007277:Injections, Jet; D008252:Macaca fascicularis; D008297:Male; D009339:Needles; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D013594:Syringes; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage*
D016207:Cytokines; D015483:HIV Antibodies; D019444:Vaccines, DNA