title: Two approaches to biological decontamination of groundwater and soil polluted by aromatics-characterization of microbial populations.
abstract: As part of the EU project MULTIBARRIERS, six new endogenous aerobic bacterial isolates able to grow in the presence of BTmX (benzene, toluene, m-xylene) were characterized with respect to their growth specificities. Preliminary analysis included restriction fragment length polymorphism profiles and 16S rDNA sequencing. The diversity of these strains was confirmed by denaturing gradient gel electrophoresis. Additional aerobic bacterial strains were isolated from the rhizospheres of plants grown in polychlorinated biphenyl (PCB)-contaminated soils. Pot experiments were designed to show the beneficial effect of plants on the bacterial degradation of PCBs. The effect of PCB removal from soil was evaluated and bacteria isolated from three different plant species were examined for the presence of the bph operon.
authors.affiliation: Dpt. of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Inst. of Chemical Technology Prague, Czech Republic. Katerina.Demnerova@vscht.cz
authors.forename: Katerina
authors.initials: K
authors.lastname: Demnerová
authors.forename: Martina
authors.initials: M
authors.lastname: Mackova
authors.forename: Veronika
authors.initials: V
authors.lastname: Spevákova
authors.forename: Katarina
authors.initials: K
authors.lastname: Beranova
authors.forename: Lucie
authors.initials: L
authors.lastname: Kochánková
authors.forename: Petra
authors.initials: P
authors.lastname: Lovecká
authors.forename: Edita
authors.initials: E
authors.lastname: Ryslavá
authors.forename: Tomas
authors.initials: T
authors.lastname: Macek
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003666:Decontamination / Q000379:methods*; D017753:Ecosystem; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology*; D010944:Plants / Q000382:microbiology; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D006841:Hydrocarbons, Aromatic; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical