title: Spectral domain second-harmonic optical coherence tomography.
abstract: Optical coherence tomography (OCT) provides micrometer-scale structural imaging by coherent detection of backscattered light. Molecular contrast in OCT has been demonstrated using transient absorption, coherent anti-Stokes Raman scattering, and second-harmonic (SH) generation. The sensitivity of molecular contrast signals can be enhanced by use of Fourier domain techniques. We have constructed a spectrometer-based Fourier domain SH-OCT system for simultaneous acquisition of the fundamental and SH signals. We report a >30 dB increase in SH sensitivity over a similar time domain SH-OCT system and demonstrate contrast between cartilage and bone using collagen as the contrast agent.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA. mvs5@duke.edu
authors.forename: Marinko V
authors.initials: MV
authors.lastname: Sarunic
authors.forename: Brian E
authors.initials: BE
authors.lastname: Applegate
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Izatt
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D008857:Microscopy, Interference / Q000295:instrumentation* / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation* / Q000379:methods; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods