title: Apolipoprotein B gene polymorphisms in people in the east Mediterranean area of Turkey.
abstract: Point mutations in the receptor binding domain of low density lipoprotein may increase cholesterol levels in blood. Three mutations of Apo B-100 protein result in defective binding (Arg 3500 ----> [corrected] Gln, Arg 3500 ----> [corrected] Trp and Arg 3531 ----> [corrected] Cys). We estimated the frequency of Apo B point mutations (codon 3500) C9774T (Arg 3500 ----> [corrected] Trp) and G9775A (Arg 3500 ----> [corrected] Gln) in 179 atherosclerotic, 145 hyperlipidaemic individuals and 272 healthy individuals in the east Mediterranean region of Turkey. Lipid and lipoprotein levels were measured with routine biochemical analyser and Apo B mutation was detected using real-time PCR. Neither mutation was found. In this region, Apo B-100 protein mutations are rare and causes of hyperlipidaemia and atherosclerosis may therefore be unrelated to them.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Mersin University, Mersin, Turkey.
authors.forename: L
authors.initials: L
authors.lastname: Tamer
authors.forename: K
authors.initials: K
authors.lastname: Tanriverdi
authors.forename: B
authors.initials: B
authors.lastname: Ercan
authors.forename: A
authors.initials: A
authors.lastname: Unlu
authors.forename: N
authors.initials: N
authors.lastname: Sucu
authors.forename: H
authors.initials: H
authors.lastname: Pekdemir
authors.forename: U
authors.initials: U
authors.lastname: Atik
mesh_terms: D000328:Adult; D053299:Apolipoprotein B-100; D001054:Apolipoproteins A / Q000097:blood; D001055:Apolipoproteins B / Q000097:blood / Q000235:genetics*; D001161:Arteriosclerosis / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D015984:Causality; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D019083:Mediterranean Region / Q000453:epidemiology; D008875:Middle Aged; D017720:Molecular Epidemiology; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics*; D011159:Population Surveillance; D035583:Rare Diseases; D014280:Triglycerides / Q000097:blood; D014421:Turkey / Q000453:epidemiology
chemical_list: D053299:Apolipoprotein B-100; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D014280:Triglycerides