Endocytosis resumes during late mitosis and is required for cytokinesis.
Recent work has underscored the importance of membrane trafficking events during cytokinesis. For example, targeted membrane secretion occurs at the cleavage furrow in animal cells, and proteins that regulate endocytosis also influence the process of cytokinesis. Nonetheless, the prevailing dogma is that endosomal membrane trafficking ceases during mitosis and resumes after cell division is complete. In this study, we have characterized endocytic membrane trafficking events that occur during mammalian cell cytokinesis. We have found that, although endocytosis ceases during the early stages of mitosis, it resumes during late mitosis in a temporally and spatially regulated pattern as cells progress from anaphase to cytokinesis. Using fixed and live cell imaging, we have found that, during cleavage furrow ingression, vesicles are internalized from the polar region and subsequently trafficked to the midbody area during later stages of cytokinesis. In addition, we have demonstrated that cytokinesis is inhibited when clathrin-mediated endocytosis is blocked using a series of dominant negative mutants. In contrast to previous thought, we conclude that endocytosis resumes during the later stages of mitosis, before cytokinesis is completed. Furthermore, based on our findings, we propose that the proper regulation of endosomal membrane traffic is necessary for the successful completion of cytokinesis.
Schweitzer
Jill Kuglin
JK
Department of Biological Sciences, University of Notre Dame, Indiana 46556-0369, USA.
Burke
Erin E
EE
Goodson
Holly V
HV
D'Souza-Schorey
Crislyn
C
D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000378:metabolism; D000705:Anaphase; D002462:Cell Membrane / Q000378:metabolism; D048750:Cell Nucleus Division; D002966:Clathrin / Q000378:metabolism; D016207:Cytokines / Q000737:chemistry; D048749:Cytokinesis; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006160:Guanosine Triphosphate / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008938:Mitosis; D008954:Models, Biological; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014168:Transferrin / Q000737:chemistry
D000089982:ADP-Ribosylation Factor 6; D002966:Clathrin; D016207:Cytokines; D014168:Transferrin; D049452:Green Fluorescent Proteins; D006160:Guanosine Triphosphate; D020727:ADP-Ribosylation Factors