[Establishment of transgenic mouse line skin-specifically expressing hCTLA4-Ig].
hCTLA4-Ig,which is capable of inhibiting T cell activation and rendering T cell to be anergy by blocking co-stimulatory signal pathway, is recognized as a potential therapeutic molecule to extend the survival of skin graft in burn medicine. To investigate whether the survival of skin graft can be further extended by skin-specific expression of hCTLA4-Ig, a skin-specific expression vector was constructed by pacing the encoding sequence of hCTLA4-Ig under the drive of K14 promoter, and a transgenic mouse line was established with the vector. RT-PCR and Northern blot assay indicated that hCTLA4-Ig was skin-specifically expressed in transgenic mice at a rather high level. Compared with the expression of GAPDH, the expression of hCTLA4-Ig remained constant through generations and over lifetime. The results of this paper indicated that a transgenic mouse line skin-specifically and constitutively expressing hCTLA4-Ig had been established.
Wang
Yong
Y
Laboratory Animal Science Department, College of Basic Medicine, Third Military Medical University, Chongqing 400038, China.
Wang
Feng-Chao
FC
Wei
Hong
H
Ni
Yong
Y
Wu
Jun
J
Gao
Xiang
X
D000069594:Abatacept; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D018796:Immunoconjugates / Q000235:genetics*; D053547:Keratin-14 / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism*; D014162:Transfection
D018796:Immunoconjugates; D053547:Keratin-14; D000069594:Abatacept