Antinociceptive anti-inflammatory effect of Monotropein isolated from the root of Morinda officinalis.
The root of Morinda officinalis (Rubiaceae) is used to treat rheumatoid arthritis and impotence in the traditional Oriental medicine. To identify the antinociceptive anti-inflammatory components of this crude drug, we adopted an activity-directed fractionation approach. The active fraction of the BuOH extract of M. officinalis root was subjected to silica gel and ODS column chromatography to yield two diterpenes, compounds 1 and 2 and these were identified as monotropein and deacetylasperulosidic acid, respectively. The iridoid glycoside, monotropein, was tested for its anti-inflammatory antinociceptive effects using hot plate- and writhing antinociceptive assays and by using carrageenan-induced anti-inflammatory assays in mice and rats. Pretreatment with monotropein (at 20, 30 mg/kg/d, p.o.) significantly reduced stretching episodes and prolonged action time in mice. It also significantly reduced acute paw edema by carrageenan in rats. These results indicate that monotropein contributes to the antinociceptive and anti-inflammatory action of Morinda officinalis root.
Choi
Jongwon
J
College of Pharmacy, Daeyeon-Dong, Kyungsung University; Busan 608-736, Korea.
Lee
Kyung-Tae
KT
Choi
Moo-Young
MY
Nam
Jung-Hwan
JH
Jung
Hyun-Ju
HJ
Park
Sun-Kyu
SK
Park
Hee-Juhn
HJ
D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002850:Chromatography, Gel; D039823:Iridoids / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D032066:Morinda / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013057:Spectrum Analysis
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D039823:Iridoids; C507582:monotropein