[In vitro study on cranial neural crest differentiating into ectomesenchymal cell of the first branchial arch by FGF-8].
OBJECTIVE
To investigate the effects of FGF-8 on cranial neural crest cell (CNCC) differentiating into ectomesenchymal cell of the first branchial arch, and determine the appropriate dose and stage of CNCC exposure to FGF-8.


METHODS
Cranial neural crest explants were cultured in free-serum medium containing modified DMEM/F12 and different doses of FGF-8. The differentiation type of CNCC were determined by in situ hybridization for Hoxa2 and immunocytochemistry for vimentin.


RESULTS
Pre-emigrating CNCC demonstrated the negative Hoxa2 stain and positive vimentin stain after treated by 100 ug/FGF-8. Both post-emigrating CNCC group and control group were positive for Hoxa2 and vimentin stain.


CONCLUSIONS
On the early stage of CNCC emigration, the first branchial arch phenotype of CNCC could be induced by FGF-8. This experiment could provide in vitro model for study on the mechanism of tooth-jaw regeneration.
Jiang
Hong-bing
HB
Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Tian
Wei-dong
WD
Tang
Wei
W
Liu
Lei
L
Li
Xiao-dong
XD
D000818:Animals; D001934:Branchial Region / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003391:Cranial Nerves / Q000166:cytology*; D005260:Female; D051524:Fibroblast Growth Factor 8 / Q000494:pharmacology*; D008297:Male; D008648:Mesoderm / Q000166:cytology*; D051379:Mice; D008815:Mice, Inbred Strains; D009432:Neural Crest / Q000166:cytology*
D051524:Fibroblast Growth Factor 8