title: Characteristics of protein partitioning in an aqueous micellar-gel system.
abstract: Partitioning of proteins has been studied experimentally in a system combining a gel-bead phase and a nonionic micellar phase. The micellar phase consists of cylindrically shaped micelles, which are completely excluded from the gel-bead phase. Partitioning of single-component protein solutions (myoglobin, ovalbumin, and BSA) is determined by excluded-volume interactions in the micellar phase, and as a result the proteins prefer the gel-bead phase to the micellar phase. The protein concentration inside the gel beads increases with an increase in volume fraction of the micelles and increases with an increase in the size of the proteins. The protein partition coefficients obtained for a binary mixture of myoglobin and bovine serum albumin (BSA) show the same protein concentration dependence as the single-component protein partition coefficients.
authors.affiliation: Department of Chemical Engineering and Chemistry, Process Development Group, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands. l.p.j.van.den.broeke@tue.nl
authors.forename: L J P
authors.initials: LJ
authors.lastname: van den Broeke
authors.forename: D
authors.initials: D
authors.lastname: van Roosmalen
authors.forename: M P J
authors.initials: MP
authors.lastname: Dohmen-Speelmans
authors.forename: C H J T
authors.initials: CH
authors.lastname: Dietz
authors.forename: L A M
authors.initials: LA
authors.lastname: van der Wielen
authors.forename: J T F
authors.initials: JT
authors.lastname: Keurentjes
mesh_terms: D045424:Complex Mixtures / Q000737:chemistry; D005782:Gels; D008823:Micelles; D008863:Microspheres; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D045424:Complex Mixtures; D005782:Gels; D008823:Micelles; D011506:Proteins; D014867:Water