[Establishment of dendritomas by fusion of human dendritic cells with human hepatocellular carcinoma cell line HLE cells].
OBJECTIVE
To construct dendritomas by fusion of human dendritic cells with HLE cells, a human hepatocellular carcinoma cell line.


METHODS
HLE cells were cultured in RPMI 1640 with 15% FCS. Human dendritic cells (DCs) were obtained from peripheral blood monocytes cultured in the presence of GM-CSF and IL-4 for 7 days, matured with TNF-alpha and PGE(2) for 2 days. The DCs and HLE cells were labeled with green fluorescence dye PKH67-GL and red fluorescence dye PKH26-GL, respectively, and fused in 50% polyethylene glycol (PEG) + 10% dimethyl sulfoxide (DMSO) to generate dendritomas for rapid fluorescence-activated cell sorting (FACS).


RESULTS
Dendritomas with dual red-green fluorescence were constructed successfully, and FACS analysis showed the effective fusion rate was 16.8%.


CONCLUSIONS
With fluorescence dyes PKH67-GL and PKH26-GL as fusion markers, dendritomas for rapid fluorescence-activated cell sorting are constructed, which may throw new light on immunotherapy of hepatocellular carcinoma.
Guan
Xin
X
Department of Hepatobiliary Surgery, Peking University People's Hospital, Beijing 100044, China.
Peng
Ji-run
JR
Leng
Xi-sheng
XS
D019496:Cancer Vaccines / Q000096:biosynthesis; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002459:Cell Fusion / Q000379:methods*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology*; D006801:Humans; D006822:Hybrid Cells; D008113:Liver Neoplasms / Q000473:pathology*
D019496:Cancer Vaccines