Low pH changes the profile of nodulation factors produced by Rhizobium tropici CIAT899.
Rhizobium tropici CIAT899 has been cataloged as a nodulator of bean, a plant often growing in areas characterized by highly acidic soils. The purpose of this work was to explore the effects of acidity on the production of Nod factors by this strain and their impact on the establishment of effective symbioses. We report that acidity increases rhizobial Nod factors production, and we exhaustively study the nodulation factor structures produced under abiotic stress. Significant differences were observed between the structures produced at acid and neutral pH: 52 different molecules were produced at acid pH, 29 at neutral pH, and only 15 are common to bacteria grown at pH 7.0 or 4.5. The results indicate that R. tropici CIAT899 has successfully adapted to life in acidic soils and is a good inoculant for the bean under these conditions.
Morón
Belén
B
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, c/ Profesor García González, Spain.
Soria-Díaz
María Eugenia
ME
Ault
James
J
Verroios
George
G
Noreen
Sadaf
S
Rodríguez-Navarro
Dulce N
DN
Gil-Serrano
Antonio
A
Thomas-Oates
Jane
J
Megías
Manuel
M
Sousa
Carolina
C
D000222:Adaptation, Physiological; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D013058:Mass Spectrometry; D008745:Methylation; D009586:Nitrogen Fixation / Q000235:genetics; D027805:Phaseolus / Q000254:growth & development / Q000382:microbiology; D042162:Rhizobium tropici / Q000378:metabolism* / Q000502:physiology
D008070:Lipopolysaccharides