title: Changes in coagulation-fibrinolysis balance in blood mononuclear cells and in gastric mucosa from patients with Helicobacter pylori infection.
abstract: BACKGROUND
  Helicobacter pylori and some of its virulence factors stimulate human blood mononuclear cells (MNC) in vitro to produce tissue factor (TF) and plasminogen activator inhibitor-2 (PAI-2). In this study we investigated the procoagulant-fibrinolytic potential of blood MNC in patients with H. pylori infection. In the same patients we also evaluated the coagulation-fibrinolysis profile in gastric tissue and in plasma.
  
  
  RESULTS
  The production of TF and PAI-2 was evaluated in 61 patients with dyspepsia, 31 positive and 30 negative for H. pylori infection. TF expressed by MNC and PAI-2 accumulation in cell culture medium after incubation for 20 h at 37 degrees C were significantly higher in H. pylori(+) than in H. pylori(-) patients and were significantly correlated. TF and PAI-2 content in extracts of gastric mucosa was similar in the two groups whereas lower levels of tissue plasminogen activator (t-PA) and thrombomodulin (TM) antigens were found in the antrum of H. pylori(+) patients. No difference between the groups was observed in plasma thrombus precursor protein, prothrombin fragment 1+2, D-dimer, t-PA, PAI-1, TM and thrombin activatable fibrinolysis inhibitor.
  
  
  CONCLUSIONS
  H. pylori infection is associated with functional abnormalities of blood MNC resulting in the coordinate expression of TF and antifibrinolytic activity. Changes in cell coagulation-fibrinolysis balance may represent a link between H. pylori infection and ischemic heart disease.
authors.affiliation: Department of Biomedical Sciences and Human Oncology, Section of General Pathology, University of Bari, Piazza G. Cesare, 11; I-70124 Bari, Italy. mario.colucci@dimo.uniba.it
authors.forename: M
authors.initials: M
authors.lastname: Colucci
authors.forename: M R
authors.initials: MR
authors.lastname: Rossiello
authors.forename: A
authors.initials: A
authors.lastname: Pentimone
authors.forename: P
authors.initials: P
authors.lastname: Berloco
authors.forename: F
authors.initials: F
authors.lastname: Russo
authors.forename: A
authors.initials: A
authors.lastname: Di Leo
authors.forename: N
authors.initials: N
authors.lastname: Semeraro
mesh_terms: D000328:Adult; D000368:Aged; D000941:Antigens / Q000032:analysis; D001706:Biopsy; D001777:Blood Coagulation / Q000502:physiology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005342:Fibrinolysis / Q000502:physiology*; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology; D005756:Gastritis / Q000378:metabolism / Q000382:microbiology; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017396:Plasminogen Activator Inhibitor 2 / Q000032:analysis / Q000096:biosynthesis; D011706:Pyloric Antrum / Q000378:metabolism; D018180:Thrombomodulin / Q000032:analysis; D010959:Tissue Plasminogen Activator / Q000032:analysis / Q000096:biosynthesis
chemical_list: D000941:Antigens; D017396:Plasminogen Activator Inhibitor 2; D018180:Thrombomodulin; D010959:Tissue Plasminogen Activator