title: Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
abstract: Recent consolidation of the whole-genome sequence with genome-wide transcriptome profiling revealed the existence of functional units within the genome in specific chromosomal regions, as seen in the coordinated expression of gene clusters and colocalization of functionally related genes. An efficient region-specific mutagenesis screen would greatly facilitate research in addressing the importance of these clusters. Here we use the 'local hopping' phenomenon of a DNA-type transposon, Sleeping Beauty (SB), for region-specific saturation mutagenesis. A transgenic mouse containing both transposon (acts as a mutagen) and transposase (recognizes and mobilizes the transposon) was bred for germ-cell transposition events, allowing us to generate many mutant mice. All genes within a 4-Mb region of the original donor site were mutated by SB, indicating the potential of this system for functional genomic studies within a specific chromosomal region.
authors.affiliation: Center for Advanced Science and Innovation, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Vincent W
authors.initials: VW
authors.lastname: Keng
authors.forename: Kojiro
authors.initials: K
authors.lastname: Yae
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Sumi
authors.initials: S
authors.lastname: Mizuno
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Uno
authors.forename: Kosuke
authors.initials: K
authors.lastname: Yusa
authors.forename: Chikara
authors.initials: C
authors.lastname: Kokubu
authors.forename: Taroh
authors.initials: T
authors.lastname: Kinoshita
authors.forename: Keiko
authors.initials: K
authors.lastname: Akagi
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: Neal G
authors.initials: NG
authors.lastname: Copeland
authors.forename: Kyoji
authors.initials: K
authors.lastname: Horie
authors.forename: Junji
authors.initials: J
authors.lastname: Takeda
mesh_terms: D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D018095:Germ-Line Mutation; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D005810:Multigene Family; D016254:Mutagenesis, Insertional / Q000379:methods*
chemical_list: D004251:DNA Transposable Elements