title: Trans fatty acids and atopic eczema/dermatitis syndrome: the relationship with a free radical cis-trans isomerization of membrane lipids.
abstract: The formation of trans FA residues in membrane phospholipids may be due to a free radical-catalyzed isomerization process occurring to the cis unsaturated FA moieties. Radical stress is well documented in inflammatory processes of atopic diseases, but no data are yet available about a possible association with trans FA detected in these patients. We investigated the presence of trans lipid isomers in the erythrocyte and T-lymphocyte membranes of 26 children affected by atopic eczema/dermatitis syndrome (AEDS). Trans lipid isomers were found in both cell membranes, up to a total content of 2.7 and 4.9% of the FA composition, respectively. By using the geometrical trans lipid library derived from in vitro models of thiyl radical-catalyzed isomerization, oleic and arachidonic acid isomers were detected. The statistical significance was evaluated by comparison with an age-matched control group. These results suggest the role of an endogenous free radical isomerization path occurring to membrane unsaturated lipids, complementary to the dietary contribution, which can be involved in the lipid impairment in AEDS. This study contributes to lipidomic research regarding the double bond structure and the influence of a geometrical change of membrane lipids in physiology and diseases.
authors.affiliation: Istituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche, 40129 Bologna, Italy. cferreri@isof.cnr.it
authors.forename: Carla
authors.initials: C
authors.lastname: Ferreri
authors.forename: Federica
authors.initials: F
authors.lastname: Angelini
authors.forename: Chryssostomos
authors.initials: C
authors.lastname: Chatgilialoglu
authors.forename: Sergio
authors.initials: S
authors.lastname: Dellonte
authors.forename: Viviana
authors.initials: V
authors.lastname: Moschese
authors.forename: Paolo
authors.initials: P
authors.lastname: Rossi
authors.forename: Loredana
authors.initials: L
authors.lastname: Chini
mesh_terms: D002849:Chromatography, Gas; D003876:Dermatitis, Atopic / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005609:Free Radicals; D006801:Humans; D007536:Isomerism; D008563:Membrane Lipids / Q000097:blood* / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D005609:Free Radicals; D008563:Membrane Lipids