title: Characterization of the naturally occurring oxacillinase of Acinetobacter baumannii.
abstract: A chromosomally encoded oxacillinase, OXA-69, was characterized from Acinetobacter baumannii AYE. beta-Lactamase OXA-69 shared 97% amino acid identity with the recently described OXA-51 enzyme of A. baumannii and 62 and 56% amino acid identity with the carbapenem-hydrolyzing oxacillinases OXA-24 and OXA-23, respectively. Biochemical characterization of the purified OXA-69 revealed a narrow-spectrum hydrolysis profile but including, at a low level, imipenem and meropenem. By PCR and sequencing bla(OXA-69)-like genes were identified in all A. baumannii strains tested (n = 12), suggesting that this oxacillinase is naturally occurring in that species.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Le Kremlin-Bicêtre, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Héritier
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Pierre-Edouard
authors.initials: PE
authors.lastname: Fournier
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Claverie
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001483:Base Sequence; D015780:Carbapenems / Q000737:chemistry / Q000494:pharmacology; D003001:Cloning, Molecular; D019281:Dimerization; D005798:Genes, Bacterial; D005843:Geography; D006868:Hydrolysis; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D010957:Plasmids; D011506:Proteins / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D001618:beta-Lactamases / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D011506:Proteins; D001618:beta-Lactamases; C108449:oxacillinase