[The role of cell junction in hypothermic impairment of tissue].
OBJECTIVE
To investigate the role of cell junction in the hypothermal impairment of tissue without any cryoprotectant.


METHODS
Epithelial MDCK cell line which is able to form well-defined cell junctions after confluency, and fibroblast V79 cell line which is unable to be used for graded freezing experiments, either in suspension, single tough attached to flasks or as monolayers on flasks. Then the viability was assessed by WST-1 and MT, respectively.


RESULTS
The results of viability of the two types of cells assessed by WST-1 were well-fitted with that of MTT. The recovery of the two types of cells in suspension was higher than that of single tough group and monolayer group. The single tough group of MDCK had a higher recovery than did the monolayer group. At the same time, such tendency was not observed on V79 cells. As compared with V79 monolayers, lower recovery was found on MDCK monolayers.


CONCLUSIONS
The junction between cells and the adhesion between cell and matrix have important roles in the hypothermic impairment of tissue. Their existence affect the recovery of cells under hypothermia. The cytoskeleton system may contribute to the impairment caused by junctions.
Jia
X
X
Burn Institute, 304th Hospital, People's Liberation Army, Bejiing 100037, China.
Ji
X
X
Yang
H
H
Ebertz
S
S
McGann
L
L
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D015925:Cryopreservation; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D005627:Frostbite / Q000503:physiopathology*; D007365:Intercellular Junctions / Q000502:physiology*