title: Schizosaccharomyces pombe mst2+ encodes a MYST family histone acetyltransferase that negatively regulates telomere silencing.
abstract: Histone acetylation and deacetylation are associated with transcriptional activity and the formation of constitutively silent heterochromatin. Increasingly, histone acetylation is also implicated in other chromosome transactions, including replication and segregation. We have cloned the only Schizosaccharomyces pombe MYST family histone acetyltransferase genes, mst1(+) and mst2(+). Mst1p, but not Mst2p, is essential for viability. Both proteins are localized to the nucleus and bound to chromatin throughout the cell cycle. Deltamst2 genetically interacts with mutants that affect heterochromatin, cohesion, and telomere structure. Mst2p is a negative regulator of silencing at the telomere but does not affect silencing in the centromere or mating type region. We generated a census of proteins and histone modifications at wild-type telomeres. A histone acetylation gradient at the telomeres is lost in Deltamst2 cells without affecting the distribution of Taz1p, Swi6p, Rad21p, or Sir2p. We propose that the increased telomeric silencing is caused by histone hypoacetylation and/or an increase in the ratio of methylated to acetylated histones. Although telomere length is normal, meiosis is aberrant in Deltamst2 diploid homozygote mutants, suggesting that telomeric histone acetylation contributes to normal meiotic progression.
authors.affiliation: Molecular & Computational Biology Section, University of Southern California, Los Angeles, 90089-2910, USA.
authors.forename: Eliana B
authors.initials: EB
authors.lastname: Gómez
authors.forename: Joaquín M
authors.initials: JM
authors.lastname: Espinosa
authors.forename: Susan L
authors.initials: SL
authors.lastname: Forsburg
mesh_terms: D000107:Acetylation; D001483:Base Sequence; D002453:Cell Cycle; D002467:Cell Nucleus / Q000201:enzymology; D002843:Chromatin / Q000201:enzymology; D004271:DNA, Fungal / Q000235:genetics; D020868:Gene Silencing; D005800:Genes, Fungal; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D008540:Meiosis / Q000235:genetics; D009154:Mutation; D010802:Phylogeny; D012568:Schizosaccharomyces / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D016615:Telomere / Q000235:genetics
chemical_list: D002843:Chromatin; D004271:DNA, Fungal; D006657:Histones