[Identification of protein peroxiredoxin 2 related to crystalline NiS-induced neoplastic transformation].
OBJECTIVE
To provide evidence for illustrating the molecular mechanism of nickel carcinogenesis, and to identify the differential expression of protein in crystalline NiS-induced neoplastic transformation of human bronchial epithelial cell by proteomics technology.


METHODS
Two dimensional electrophoresis (2-DE) and the ImageMaster 3.10 software were used to analyze the differential expression of protein, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) combined with database search was applied to identify protein peroxiredoxin 2 (PDX2) related to malignant transformation.


RESULTS
The good 2-DE pattern including resolution and reproducibility was obtained. Nearly 700 expressed proteins per 2-D gel were isolated with molecular weights (MW) ranging from 14,400 to 94,000 KD and pI 3 - 10. A protein PDX2 with MW 21,890 KD, pI 5.66, which was highly expressed in malignantly transformed cell, was identified using MALDI-TOF-MS.


CONCLUSIONS
PDX2 was involved in malignant transformation of human bronchial epithelial cell induced by crystalline nickel sulfide.
Ji
Wei-dong
WD
Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou 510182, China.
Chen
Jia-kun
JK
Lü
Jia-chun
JC
Wu
Zhong-liang
ZL
Feng
Su-mei
SM
Yi
Fei
F
D001980:Bronchi / Q000166:cytology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D009532:Nickel / Q000633:toxicity*; D054464:Peroxiredoxins / Q000378:metabolism*; D020543:Proteome
D020543:Proteome; D009532:Nickel; C086065:PRDX2 protein, human; D054464:Peroxiredoxins; C017558:nickel sulfide