title: Albumin attenuates neutrophil activation induced by stimulators including antibodies against neutrophil-specific antigens.
abstract: As neutrophil activation is related to several adverse transfusion reactions, we studied about the activation induced by anti-neutrophil antibodies and the stabilizing effects of albumin pretreatment by means of flow cytometry. Anti-neutrophil monoclonal antibody (anti-HNA-1a, 1b, 2a) alone induced CD11b expression and shedding of L-selectin, and anti-HNA-1a reinforced reactive oxygen species (ROS) production. Albumin pretreatment significantly reduced CD11b expression and L-selectin shedding induced by fMLP and ROS production induced by PMA, G-CSF combined with PMA or LPS-fMLP, or anti-HNA-1a combined with PMA. These findings suggest that anti-HNA-1a is related to adverse reactions and albumin has a regulating effect on neutrophil activation.
authors.affiliation: Blood Transfusion Division, Kobe University Hospital, Chuo-ku, Kobe 650-0017, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Katsuyasu
authors.initials: K
authors.lastname: Saigo
authors.forename: Yukie
authors.initials: Y
authors.lastname: Jyokei
authors.forename: Manami
authors.initials: M
authors.lastname: Kishimoto
authors.forename: Mariko
authors.initials: M
authors.lastname: Takenokuchi
authors.forename: Nobuo
authors.initials: N
authors.lastname: Araki
authors.forename: Shion
authors.initials: S
authors.lastname: Imoto
authors.forename: Kikuyo
authors.initials: K
authors.lastname: Taniguchi
authors.forename: Shunichi
authors.initials: S
authors.lastname: Kumagai
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D039481:CD11b Antigen / Q000096:biosynthesis*; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D019041:L-Selectin / Q000276:immunology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000276:immunology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects* / Q000276:immunology; D009504:Neutrophils / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D039481:CD11b Antigen; D002273:Carcinogens; D008562:Membrane Glycoproteins; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D019041:L-Selectin; D016179:Granulocyte Colony-Stimulating Factor; D009240:N-Formylmethionine Leucyl-Phenylalanine; D013755:Tetradecanoylphorbol Acetate