Determination of cardiac troponin I for the auxiliary diagnosis of acute myocardial infarction by anodic stripping voltammetry at a carbon paste electrode.
An electrochemical immunoassay for cardiac troponin I (cTnI) combining the concepts of the dual monoclonal antibody "sandwich" principle, the silver enhancement on the nano-gold particle, and the anodic stripping voltammetry is described. Four main steps were carried out to obtain the analytical signal, i.e., electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. A linear relationship between the anodic stripping peak current and concentration of cTnl from 1 to 20 ng/ml and a limit of detection of 0.8 ng/ml were obtained. The established method was tested by determining cTnI in acute myocardial infarction (AMI) samples using enzyme-linked immunoadsorbent assay (ELISA) for comparison analysis, and good results were obtained.
Guo
Huishi
H
Chien-Shiung Wu Laboratory, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P R. China.
Zhang
Jinan
J
Xiao
Pengfeng
P
Nie
Libo
L
Yang
Di
D
He
Nongyue
N
D000911:Antibodies, Monoclonal; D002244:Carbon; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D017979:Gold Colloid; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000378:metabolism*; D009206:Myocardium / Q000737:chemistry*; D049329:Nanostructures; D036103:Nanotechnology; D019210:Troponin I / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D019210:Troponin I; D002244:Carbon