FAK and PYK2 interact with SAP90/PSD-95-Associated Protein-3.
Focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2) are two related non-receptor tyrosine kinases highly expressed in brain. Although they are both involved in synaptic plasticity, little is known about their specific neuronal partners. Using a yeast two-hybrid screen and GST pull-down assays we show that SAPAP3 (SAP90/PSD-95-Associated Protein-3) interacts with FAK (residues 676-840) and PYK2. The three proteins partly co-distribute in the same sucrose gradient fractions as the post-synaptic density protein PSD-95 and Src. Our results suggest that SAPAP3 is an anchoring protein for FAK and PYK2 in post-synaptic densities and may contribute to the synaptic function of these tyrosine kinases.
Bongiorno-Borbone
Lucilla
L
INSERM, U536, Paris, France.
Kadaré
Gress
G
Benfenati
Fabio
F
Girault
Jean-Antoine
JA
D000818:Animals; D001921:Brain / Q000201:enzymology*; D019556:COS Cells; D002499:Centrifugation, Density Gradient; D002522:Chlorocebus aethiops; D004789:Enzyme Activation; D051418:Focal Adhesion Kinase 2 / Q000378:metabolism*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D000074407:SAP90-PSD95 Associated Proteins; D013347:Subcellular Fractions; D013569:Synapses / Q000737:chemistry / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism
D009419:Nerve Tissue Proteins; D000074407:SAP90-PSD95 Associated Proteins; D014443:Tyrosine; D051418:Focal Adhesion Kinase 2; D051416:Focal Adhesion Protein-Tyrosine Kinases