In vivo confocal imaging of the retina in animal models using scanning laser ophthalmoscopy.
Scanning-laser ophthalmoscopy is a technique for confocal imaging of the eye in vivo. The use of lasers of different wavelengths allows to obtain information about specific tissues and layers due to their reflection and transmission characteristics. In addition, fluorescent dyes excitable in the blue and infrared range offer a unique access to the vascular structures associated with each layer. In animal models, a further enhancement in specificity can be obtained by GFP expression under control of tissue-specific promotors. Important fields of application are studies in retinal degenerations and the follow-up of therapeutic intervention.
Seeliger
Mathias W
MW
Retinal Diagnostics Research Group, Tuebingen, Germany. see@uni-tuebingen.de
Beck
Susanne C
SC
Pereyra-Muñoz
Naira
N
Dangel
Susann
S
Tsai
Jen-Yue
JY
Luhmann
Ulrich F O
UF
van de Pavert
Serge A
SA
Wijnholds
Jan
J
Samardzija
Marijana
M
Wenzel
Andreas
A
Zrenner
Eberhart
E
Narfström
Kristina
K
Fahl
Edda
E
Tanimoto
Naoyuki
N
Acar
Niyazi
N
Tonagel
Felix
F
D000818:Animals; D005451:Fluorescein Angiography; D007208:Indocyanine Green; D007834:Lasers; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D023421:Models, Animal; D009887:Ophthalmoscopy / Q000379:methods*; D012160:Retina / Q000473:pathology*; D012164:Retinal Diseases / Q000473:pathology*
D007208:Indocyanine Green