Analytical and biological evaluation of high throughput screen actives using evaporative light scattering, chemiluminescent nitrogen detection, and accurate mass LC-MS-MS.
In this investigation the utility of evaporative light scattering detection (ELSD) combined with HPLC-MS was demonstrated as a key component of a bioassay-guided fractionation, or "biofractionation" technique, for the evaluation of high throughput screen actives. ELSD provided on-line analyte mass information that was critical for the classification of the samples. Chemiluminescent nitrogen detection (CLND) was also evaluated for sample concentration estimation for nitrogen-containing compounds, and accurate mass LC-MS-MS analysis was employed for rapid structural confirmation and elucidation of components previously identified as active via biofractionation.
Peake
D A
DA
Lilly Research Laboratories, A Division of Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN 46285, USA.
Duckworth
D C
DC
Perun
T J
TJ
Scott
W L
WL
Kulanthaivel
P
P
Strege
M A
MA
D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008027:Light; D008163:Luminescent Measurements; D013058:Mass Spectrometry / Q000379:methods*; D009584:Nitrogen / Q000032:analysis*; D012542:Scattering, Radiation
D009584:Nitrogen