title: Efficient terpene hydroxylation catalysts based upon P450 enzymes derived from actinomycetes.
abstract: The hydroxylation of alpha-ionone 1 and beta-ionone 2 to their corresponding mono-hydroxylated derivatives has been examined using a recombinant E. coli whole cell system, in which cytochromes P450 SU1 and SU2, and P450 SOY were over-expressed with their cognate ferrodoxins. Both substrates are hydroxylated with a high degree of regioselectivity and for alpha-ionone 1 the reaction is highly diastereoselective yielding the anti-isomer.
authors.affiliation: School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JJ, UK.
authors.forename: Ayhan
authors.initials: A
authors.lastname: Celik
authors.forename: Sabine L
authors.initials: SL
authors.lastname: Flitsch
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turner
mesh_terms: D039903:Actinobacteria / Q000201:enzymology*; D001711:Biotransformation; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000502:physiology*; D006900:Hydroxylation; D045792:Norisoprenoids / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D045792:Norisoprenoids; D003577:Cytochrome P-450 Enzyme System; C008157:beta-ionone; C011879:alpha-ionone