Alterations in protein kinase A and different protein kinase C isoforms in the heart during morphine withdrawal.
The present study was designed to investigate the possible changes of protein kinase A (PKA) and different isoforms of protein kinase C (PKC): PKC alpha, PKC delta and PKC zeta after naloxone induced morphine withdrawal in the heart. Male rats were implanted with placebo (naïve) or morphine (tolerant/dependent) pellets for 7 days. On day 8 rats received saline s.c. or naloxone (5 mg/kg s.c.). The protein levels of PKA, PKC delta and PKC zeta were significantly up-regulated in the heart from morphine withdrawal rats. By contrast, morphine withdrawal induced down-regulation of PKC alpha. These results suggest that both PKA and PKC may be involved in the cardiac adaptive changes observed during morphine withdrawal.
Cerezo
Manuela
M
Equip of Cellular and Molecular Pharmacology, University School of Medicine, Murcia, Spain.
Milanés
M Victoria
MV
Laorden
M Luisa
ML
D000818:Animals; D015153:Blotting, Western; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D009021:Morphine Dependence / Q000150:complications*; D009206:Myocardium / Q000201:enzymology*; D009270:Naloxone / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013375:Substance Withdrawal Syndrome / Q000201:enzymology / Q000209:etiology
D007527:Isoenzymes; D009270:Naloxone; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C