[The effect of MSC on cytokine production by naive T cell differentiated in vitro].
OBJECTIVE
To investigate the effect of mesenchymal stem cell (MSC) on naive T cell and to explore its mechanism of immunoregulation.


METHODS
After culturing for 3 passages, MSC was incubated with naive T cells differentiated from cord blood CD34(+) cells in vitro. Variance of cytokine produced by naive T cell in culture supernatant was analyzed by enzyme-linked immunoassays.


RESULTS
On the 7th day of co-culture, a mild proliferation of T cells in the co-culture group was observed: (9.15 +/- 0.68) x 10(5)/well in MSCs + naive T cells group versus (4.87 +/- 1.33) x 10(5)/well in naive T cells alone group (P < 0.05). IFN-gamma production was lower in MSCs + naive T cells group than that in naive T cells alone group: (1.147 +/- 0.181) pg/ml versus (4.897 +/- 0.189) pg/ml (P < 0.05), but IL-2 production was higher in the co-culture group: (16.141 +/- 2.729) pg/ml versus (2.551 +/- 0.460) pg/ml (P < 0.05). Neither IL-4 nor IL-10 were detected.


CONCLUSIONS
MSC have allogeneic effect on naive T cell, but may suppress alloreactive T lymphocyte and reduce the incidence of GVHD partly by decreased IFN-gamma production. The result may provide new clues for explaining immunoregulatory mechanism of MSC.
Jin
Jian-gang
JG
Hematopoietic Stem Cell Transplant Center of PLA, Affiliated Hospital of Chinese Academy of Miliary Medical Sciences 100039, China.
Hu
Jiang-wei
JW
Ning
Hong-mei
HM
Feng
Kai
K
Chen
Hu
H
D001854:Bone Marrow Cells / Q000276:immunology*; D002450:Cell Communication; D002478:Cells, Cultured; D018920:Coculture Techniques; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D013601:T-Lymphocytes / Q000378:metabolism*
D007378:Interleukins; D007371:Interferon-gamma