title: Neuronal expression of keratinocyte-associated transmembrane protein-4, KCT-4, in mouse brain and its up-regulation by neurite outgrowth of Neuro-2a cells.
abstract: One group of proteins that regulates neurite outgrowth and maintains neuronal networks is the immunoglobulin superfamily (IgSF). We previously identified a new member of the IgSF, keratinocyte-associated transmembrane protein-4 (KCT-4), by the signal sequence-trap method from primary cultured human keratinocytes. The KCT-4 mRNA has been found to be highly expressed in the adult human brain, although it is also distributed in various tissues. In the present study, to gain insight into the role of KCT-4 in the nervous system, we examined the expression profile and localization of KCT-4 mRNA in mouse brain. We also evaluated changes in KCT-4 mRNA expression in the differentiation of the neuroblastoma cell line Neuro-2a as the in vitro model of neurite outgrowth. KCT-4 mRNA was detected broadly in various regions of the adult mouse brain by RT-PCR. In situ hybridization revealed that it was expressed highly selectively by neurons but not by glial cells. Moreover, expression of KCT-4 mRNA was induced by neurite outgrowth of Neuro-2a. These data suggest that KCT-4 participates in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
authors.affiliation: Department of Veterinary Clinical Pathology, Nippon Veterinary and Animal Science University, Musashino-shi, Tokyo 180-8602, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamada
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Tamura
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yagihara
authors.forename: Mayu
authors.initials: M
authors.lastname: Isotani
authors.forename: Tsukimi
authors.initials: T
authors.lastname: Washizu
authors.forename: Makoto
authors.initials: M
authors.lastname: Bonkobara
mesh_terms: D000818:Animals; D015703:Antigens, CD; D015152:Blotting, Northern / Q000379:methods; D001921:Brain / Q000166:cytology*; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D008565:Membrane Proteins; D051379:Mice; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D009447:Neuroblastoma / Q000503:physiopathology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D009418:S100 Proteins / Q000378:metabolism; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D015703:Antigens, CD; D005904:Glial Fibrillary Acidic Protein; C440028:IGSF8 protein, human; D007136:Immunoglobulins; D008565:Membrane Proteins; D012333:RNA, Messenger; D009418:S100 Proteins; C489950:S100a3 protein, mouse; D014212:Tretinoin; D010751:Phosphopyruvate Hydratase