title: Defective glycosylation of cholesteryl ester transfer protein in plasma from alcohol abusers.
abstract: OBJECTIVE
  Alcohol consumption reduces the carbohydrate content of some glycoproteins, e.g. carbohydrate-deficient transferrin. The aim of this study was to investigate if there is such an alcohol-induced glycosylation defect in plasma cholesteryl ester transfer protein (CETP). A defect in the posttranslational glycosylation of CETP may affect its structure and electrical charge and may therefore affect its function. CETP activity is low in alcohol abusers.
  
  
  METHODS
  We studied the effect of alcohol consumption on CETP properties in 10 alcohol abusers and 10 control subjects. CETP was partially purified from lipoprotein-free plasma by FPLC using a Phenyl-Sepharose column. Isoelectric focusing, polyacrylamide gel electrophoresis, and western blotting were performed for partially purified CETP.
  
  
  RESULTS
  CETP had a lower molecular weight in the alcohol abusers than in the controls (range 50.6-84.0 kDa in the alcohol abusers vs 51.3-85.0 kDa in the controls). CETP purified from alcohol abusers had a higher isoelectric point, indicating a lower negative charge on the surface of the protein than in the controls' CETP. A similar effect was observed when control CETP was incubated with neuraminidase, an enzyme which is known to remove sialic acid from glycoproteins.
  
  
  CONCLUSIONS
  We conclude that CETP from alcohol abusers may have a glycosylation defect due to defective sialylation caused posttranslationally by alcohol itself or its metabolite acetaldehyde. The defective glycosylation of CETP associated with altered binding to lipoproteins may lead to the low CETP activity observed previously in alcoholic subjects.
authors.affiliation: Department of Internal Medicine, University of Oulu, PO Box 5000, 90014 Oulu, Finland. johanna.liinamaa@oulu.fi
authors.forename: M Johanna
authors.initials: MJ
authors.lastname: Liinamaa
authors.forename: Minna L
authors.initials: ML
authors.lastname: Hannuksela
authors.forename: Maria E
authors.initials: ME
authors.lastname: Rämet
authors.forename: Markku J
authors.initials: MJ
authors.lastname: Savolainen
mesh_terms: D000437:Alcoholism / Q000097:blood* / Q000276:immunology / Q000503:physiopathology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002352:Carrier Proteins / Q000097:blood*; D053480:Cholesterol Ester Transfer Proteins; D008076:Cholesterol, HDL / Q000097:blood; D006023:Glycoproteins / Q000097:blood*; D006031:Glycosylation; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male
chemical_list: D000911:Antibodies, Monoclonal; C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D008076:Cholesterol, HDL; D006023:Glycoproteins; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL