Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit.
The Lightcycler Staphylococcus kit is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers.
Choudhurry
Roos S R
RS
Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands.
Melles
Damian C
DC
Eadie
Kimberly
K
Vos
Margreet
M
Wertheim
Heiman F L
HF
Verbrugh
Henry A
HA
van Belkum
Alex
A
van Leeuwen
Willem B
WB
D002353:Carrier State / Q000382:microbiology*; D006801:Humans; D009296:Nasal Cavity / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*
D011933:Reagent Kits, Diagnostic