title: [Anti-tumor immune response in vitro induced by fusion of Tca8113 cells with macrophages].
abstract: OBJECTIVE
  To evaluate the therapeutic effectiveness of fusion tumor vaccine in tongue cancer treatment.
  
  
  METHODS
  Human macrophages fused with human tongue carcinoma cell line Tca8113 cell. The fusion cells were selected by magnetic cell sorting (MACS) and cultured. The biological properties of fusion cells and anti-tumor immune response in vitro induced by fusions were observed.
  
  
  RESULTS
  In contrast to Tca8113, the fused cells grew significantly slow in vitro. The expression of MHC I, II antigen of the fusion cells which was detected by flow cytometry (FCM) was higher than that of Tca8113. The fused cells significantly increased the proliferation of mixed lymphocyte and induced their cytotoxicity on parental Tca8113.
  
  
  CONCLUSIONS
  The fusion tumor vaccine of macrophages and OSCC cells increase in vitro immunogenicity significantly. This indicates that fusion tumor vaccine could be a new method of anti-tumor immunotherapy, which has important potentials for effective individualized human OSCC vaccine.
authors.affiliation: Department of Stomatology, 91st Hospital of PLA, Jiaozuo Henan 454100, China.
authors.forename: Ying-chun
authors.initials: YC
authors.lastname: Bi
authors.forename: Lian-jia
authors.initials: LJ
authors.lastname: Yang
authors.forename: Shao-zhong
authors.initials: SZ
authors.lastname: Dong
authors.forename: Qing-yu
authors.initials: QY
authors.lastname: Fan
authors.forename: Dian-zhong
authors.initials: DZ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002459:Cell Fusion; D045744:Cell Line, Tumor; D006649:Histocompatibility Antigens / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology*; D051381:Rats; D014062:Tongue Neoplasms / Q000276:immunology*
chemical_list: D019496:Cancer Vaccines; D006649:Histocompatibility Antigens