Resistance to reinfection in rats induced by irradiated metacercariae of Clonorchis sinensis.
A study was made to observe the association between the resistance to reinfection induced by irradiated metacercariae (MC) of Clonorchis sinensis and antigen specific Th1- and Th2-type cytokine productions in rats. Rats were infected with 20 MC of C. sinensis, previously exposed to a single dose of gamma irradiation, which varied from 0 to 100 Gy. All of them, single dose of 12 Gy showed higher IgG antibody titer with lowest worm recovery. Thus, 50 MC were used to challenge infection in rats previously infected with 20 MC irradiated at 12 Gy and the highest resistance to challenge infection was observed. The results of lymphocyte proliferation with specific antigen, ES Ag were shown no difference of proliferative responses as compared with primary and challenge infection at 12 Gy irradiation dose. In the case of cytokines production were observed that interferon (IFN-gamma) and interlukin (IL-2) were significantly enhanced, while IL-4 and IL-10 was almost unchanged to make comparison between primary and secondary infection at 12 Gy irradiation dose. In conclusion, the single dose of 12 Gy could be adopted for induction of the highest resistance to challenge infection. Up-regulation of Th1 type cytokines, IFN-gamma and IL-2 may be affected to develop vaccine by irradiated MC.
Quan
Fu Shi
FS
Microbiology and Immunology, School of Medicine, Emory University, Atlanta, GA, USA. leejb@sch.ac.kr
Lee
Jeong Beom
JB
Bae
Jun Sang
JS
Ohwatari
Nobu
N
Min
Young Ki
YK
Yang
Hun Mo
HM
D000818:Animals; D003003:Clonorchiasis / Q000276:immunology / Q000469:parasitology*; D003004:Clonorchis sinensis / Q000472:pathogenicity / Q000528:radiation effects*; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D005720:Gamma Rays; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D011829:Radiation Dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D016207:Cytokines; D007074:Immunoglobulin G