title: Genetic diversity and genetic structure of an endangered species, Trillium tschonoskii.
abstract: The genetic diversity and genetic structure of Trillium tschonoskii (Maxim) were investigated using amplified fragment length polymorphism markers. Eight primer combinations were carried out on 105 different individuals sampled from seven populations. Of the 619 discernible DNA fragments generated, 169 (27.3%) were polymorphic. The percentage of polymorphic bands within populations ranged from 4.52 to 10.50. Genetic diversity (H(E)) within populations ranged from 0.0130 to 0.0379, averaging 0.0536 at the species level. Genetic differentiation among populations was detected based on Nei's genetic diversity analysis (53.03%) and analysis of molecular variance (AMOVA) (52.43%). AMOVA indicated significant genetic differentiation among populations (52.43% of the variance) and within populations (47.57% of the variance) (p < 0.0002). Gene flow was low (0.4429) among populations. Species breeding system and limited gene flow among populations are plausible reasons for the high genetic differentiation observed for this species. We propose an appropriate strategy for conserving the genetic resources of T. tschonoskii in China.
authors.affiliation: Life Sciences, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.forename: Meng
authors.initials: M
authors.lastname: Xiao
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Yan
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
mesh_terms: D002681:China; D003247:Conservation of Natural Resources; D018744:DNA, Plant; D051456:Gene Flow; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D010936:Plant Extracts / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D031446:Trillium / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D010936:Plant Extracts