Molecular evolution perspectives on intraspecific lateral DNA transfer of topoisomerase and gyrase loci in Streptococcus pneumoniae, with implications for fluoroquinolone resistance development and spread.
Fluoroquinolones are an important class of antibiotics for the treatment of infections arising from the gram-positive respiratory pathogen Streptococcus pneumoniae. Although there is evidence supporting interspecific lateral DNA transfer of fluoroquinolone target loci, no studies have specifically been designed to assess the role of intraspecific lateral transfer of these genes in the spread of fluoroquinolone resistance. This study involves a comparative evolutionary perspective, in which the evolutionary history of a diverse set of S. pneumoniae clinical isolates is reconstructed from an expanded multilocus sequence typing data set, with putative recombinants excluded. This control history is then assessed against networks of each of the four fluoroquinolone target loci from the same isolates. The results indicate that although the majority of fluoroquinolone target loci from this set of 60 isolates are consistent with a clonal dissemination hypothesis, 3 to 10% of the sequences are consistent with an intraspecific lateral transfer hypothesis. Also evident were examples of interspecific transfer, with two isolates possessing a parE-parC gene region arising from viridans group streptococci. The Spain 23F-1 clone is the most dominant fluoroquinolone-nonsusceptible clone in this set of isolates, and the analysis suggests that its members act as frequent donors of fluoroquinolone-nonsusceptible loci. Although the majority of fluoroquinolone target gene sequences in this set of isolates can be explained on the basis of clonal dissemination, a significant number are more parsimoniously explained by intraspecific lateral DNA transfer, and in situations of high S. pneumoniae population density, such events could be an important means of resistance spread.
Stanhope
Michael J
MJ
Bioinformatics, GlaxoSmithKline, Collegeville, Pennsylvania 19426, USA. mjs297@cornell.edu
Walsh
Stacey L
SL
Becker
Julie A
JA
Italia
Michael J
MJ
Ingraham
Karen A
KA
Gwynn
Michael N
MN
Mathie
Tom
T
Poupard
James A
JA
Miller
Linda A
LA
Brown
James R
JR
Amrine-Madsen
Heather
H
D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000494:pharmacology; D001483:Base Sequence; D027081:DNA Gyrase / Q000235:genetics; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D019143:Evolution, Molecular; D024841:Fluoroquinolones / Q000494:pharmacology; D022761:Gene Transfer, Horizontal; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II