Chemiluminescence-based electrophoretic mobility shift assay of RNA-protein interactions: application to binding of viral capsid proteins to RNA.
A chemiluminescence electrophoretic mobility shift assay was introduced for the study of RNA-protein interactions that include association of genomic RNA and viral capsid proteins. Binding of the capsid protein of Venezuelan equine encephalitis virus (VEEV) to several types of RNA was used as a model system to test the application of the method. The effects of RNA secondary structures and the significance of electrostatic interaction on binding were identified. This method may have wide application to the study of RNA-protein interactions.
Kang
Jonghoon
J
Sealy Center for Structural Biology and Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, TX 77555, USA.
Lee
Myung Soog
MS
Watowich
Stanley J
SJ
Gorenstein
David G
DG
D036022:Capsid Proteins / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D004666:Encephalitis Virus, Venezuelan Equine / Q000378:metabolism; D049449:Luminescence; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000378:metabolism*
D036022:Capsid Proteins; D012313:RNA