title: [Identification of trehalose-phosphate phosphatase associated with drug-resistance from culture supernatants of isoniazid-resistant Mycobacterium tuberculosis].
abstract: OBJECTIVE
  To isolate and identify a new protein involved in drug-resistance from culture supernatants of isoniazid (INH)-resistant Mycobacterium tuberculosis.
  
  
  METHODS
  Bovine albumin of Middlebrook 7H10 culture supernatants of INH-resistant Mycobacterium tuberculosis was removed using diethylamine ethylcellulose affinitive gel chromatography. A specific protein from culture supernatants of three INH-resistant Mycobacterium tuberculosis isolates was separated reproducibly by high performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide electrophoresis, and identified by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-MS).
  
  
  RESULTS
  The protein was trehalose-phosphate phosphatase involved in the biosynthesis of trehalose of cell wall, molecular a eight 46000, isoelectric point 5.3. It could be detected repeatedly from supernatants of three INH resistant Mycobacterium tuberculosis isolates.
  
  
  CONCLUSIONS
  Trehalose-phosphate phosphatase represents an excellent potential target site for chemotherapy against Mycobacterium tuberculosis and a new marker in the detection of drug resistance of Mycobacterium tuberculosis.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Llife Science, Fudan University, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yue
authors.forename: Li-rong
authors.initials: LR
authors.lastname: Liu
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Xie
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Lei
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Hong-hai
authors.initials: HH
authors.lastname: Wang
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology*; D002417:Cattle; D004269:DNA, Bacterial; D007538:Isoniazid / Q000494:pharmacology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D010744:Phosphoric Monoester Hydrolases / Q000302:isolation & purification*; D018088:Tuberculosis, Multidrug-Resistant
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial; C023178:trehalose-phosphatase; D010744:Phosphoric Monoester Hydrolases; D007538:Isoniazid