Proposed model for the flagellar rotary motor.
Flagellated bacteria swim by rotating helical filaments driven by motors embedded in the cell wall and cytoplasmic membrane. A model is proposed to explain the mechanism of the motor. The protons passing through the channels induce a strong electric field in Mot molecules. This field originates an impulse force to cause the flagellar rotation if the following conditions are fulfilled: (a) Mot molecules have a spontaneous electric polarization. (b) The lipid bilayers are viscoelastic. (c) There is a delay of deformation in response to stress in Mot molecules. The conclusions driven from the model are in agreement with the following experimental observations, denoting the flagellar rotation velocity as omega. (1) The torque is practically constant independent of omega from 0 to a critical value omega(cr) and then decreases sharply. (2) When omega is smaller than omega(cr), the torque varies little with temperature. (3) The critical velocity omega(cr) shifts to lower speed at lower temperatures. (4) Where omega is larger than omega(cr), declining of the torque steepens at lower temperatures. (5) When omega is smaller than omega(cr), one revolution of the flagellar rotation consists of a constant number of steps. (6) When omega is smaller than omega(cr), omega is proportional to the transmembrane potential difference. (7) The stator produces constant torque even when the stator is rotated relative to the rotor by external forces. (8) How the flagellar rotation velocity changes when the direction of the proton passage is reversed. (9) The motor has a switch that reverses the sense of the flagelllar rotation with the same absolute value of torque.
Mitsui
Toshio
T
Nakasuji-Yamate 3-6-24, Takarazuka 665-0875, Japan. t-mitsui@jttk.zaq.ne.jp
Ohshima
Hiroyuki
H
D018407:Bacterial Physiological Phenomena; D001426:Bacterial Proteins / Q000737:chemistry; D001696:Biomechanical Phenomena; D055592:Biophysical Phenomena; D001703:Biophysics; D002462:Cell Membrane / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D003198:Computer Simulation; D003593:Cytoplasm / Q000378:metabolism; D005407:Flagella / Q000378:metabolism / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers; D008055:Lipids / Q000737:chemistry; D040542:Mechanotransduction, Cellular; D008564:Membrane Potentials; D008954:Models, Biological; D015233:Models, Statistical; D020409:Molecular Motor Proteins; D006180:Proton-Translocating ATPases / Q000737:chemistry; D011522:Protons; D013696:Temperature
D001426:Bacterial Proteins; D008051:Lipid Bilayers; D008055:Lipids; D020409:Molecular Motor Proteins; D011522:Protons; D006180:Proton-Translocating ATPases