title: The effect of Glucantime on the labeling of blood constituents with technetium-99m.
abstract: OBJECTIVE
  The labeling of red blood cells (C) with 99mTc is employed in clinical nuclear medicine for a variety of diagnostic procedures. Drugs can alter this labeling method and modify the disposition of the radiopharmaceuticals. In this paper, the influence of glucantime on the labeling of blood constituents with 9mTc was reported.
  
  
  METHODS
  Blood was withdrawn from rats and incubated with glucantime. Stannous chloride and 99mTc were added. After centrifugation, plasma (P) and (C) were isolated. Samples of P and C were precipitated with TCA 5%, centrifuged and insoluble (IF) and soluble fractions (SF) separated. The percentages of total activity injected (%ATI) in C, IF-P and IF-C were calculated (p < 0.05).
  
  
  RESULTS
  The %ATI on C decreased from control to following concentrations of glucantime (6.25%; 12.5%; 25%; 50%; 100%), respectively: 94.06 +/- 1.29 (control) to 77.15 +/- 2.79; to 76.68 +/- 1.88; to 75.15 +/- 2.79; to 72.64 +/- 4.40 and to 63.05 +/- 3.84. On IF-C the %ATI decreased from control to all the concentrations of glucantime (3.125%;6.25%; 12.5%; 25%; 50%; 100%), respectively: 93.34 +/- 1.18 (control) to 78.81 +/- 2.76; to 74.76 +/- 4.82; to 74.02 +/- 5.32; to 64.35 +/- 4.82; to 62.81 +/- 1.97 and to 54.55 +/- 3.58.
  
  
  CONCLUSIONS
  This effect was probably due to products present in this drug that may complex with ions (Sn(+2) and 99mTcO4) or have a direct or indirect effect on intracellular stannous ion concentration.
authors.affiliation: Universidade Federal do Rio Grande do Norte (UFRN), Centro de Biociências, Departamento de Microbiologia e Parasitologia, Av. Salgado Filho, no 3000 - 59078-970, Natal/RN, Brazil. cechol@ufrnet.br
authors.forename: Cecília Maria Carvalho Xavier
authors.initials: CM
authors.lastname: Holanda
authors.forename: Rodrigo Carvalho Holanda
authors.initials: RC
authors.lastname: Leite
authors.forename: Maria Teresa Jansem
authors.initials: MT
authors.lastname: Catanho
authors.forename: Grace Maria Lima
authors.initials: GM
authors.lastname: Souza
authors.forename: Mário
authors.initials: M
authors.lastname: Bernardo Filho
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D004912:Erythrocytes / Q000000981:diagnostic imaging* / Q000187:drug effects; D007553:Isotope Labeling; D008297:Male; D008536:Meglumine / Q000097:blood / Q000494:pharmacology*; D000077485:Meglumine Antimoniate; D009942:Organometallic Compounds / Q000097:blood / Q000494:pharmacology*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D018709:Statistics, Nonparametric; D013667:Technetium / Q000097:blood / Q000493:pharmacokinetics*; D017971:Tin Compounds / Q000097:blood; D014018:Tissue Distribution / Q000528:radiation effects
chemical_list: D001798:Blood Proteins; D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; D017971:Tin Compounds; C023599:stannous chloride; D008536:Meglumine; D013667:Technetium; D000077485:Meglumine Antimoniate