title: High performance liquid chromatographic determination of platinum in blood and urine samples of cancer patients after administration of cisplatin drug using solvent extraction and N,N'-bis(salicylidene)-1,2-propanediamine as complexation reagent.
abstract: A high performance liquid chromatographic (HPLC) procedure has been developed for the determination of cisplatin, based on the pre-column derivation of platinum(II) with reagent N,N'-bis(salicylidene)-1,2-propanediamine (H2SA2pn). The neutral platinum complex was extracted, concentrated in an organic solvent and then injected (5 microl) on a reverse phase HPLC column, Varian Micro-Pak SP C-18, 5 microm (150 mm x 4.0 mm i.d.). The complex was eluted isocratically using a ternary mixture of methanol/acetonitrile/water (40/30/30, v/v/v) at a flow rate of 1.0 ml/min and was determined by a UV detector set at 254 nm after elution. A detection limit was found to be 4.0 ng per injection. The amounts of platinum in blood serum and urine of cancer patients after administration of cisplatin were observed in a range of 221-298 ng/ml and 43-97 ng/ml with relative standard deviation (R.S.D.) of 3.6-4.6% and 3.5-4.8%, respectively. Preliminary metabolism profiles of Pt concentrations in blood and urine from the patients were established.
authors.affiliation: Department of Chemistry, the University of Western Ontario, London, Ont., Canada N6A 5B7.
authors.forename: Shah Nawaz
authors.initials: SN
authors.lastname: Lanjwani
authors.forename: Renkang
authors.initials: R
authors.lastname: Zhu
authors.forename: Mohammad Yar
authors.initials: MY
authors.lastname: Khuhawar
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Ding
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood / Q000627:therapeutic use / Q000652:urine; D002614:Chelating Agents; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002945:Cisplatin / Q000097:blood / Q000627:therapeutic use / Q000652:urine; D003959:Diamines; D006801:Humans; D007202:Indicators and Reagents; D009369:Neoplasms / Q000097:blood* / Q000188:drug therapy / Q000652:urine*; D010984:Platinum / Q000097:blood* / Q000652:urine*; D012545:Schiff Bases; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D002614:Chelating Agents; D003959:Diamines; D007202:Indicators and Reagents; C519577:N,N'-bis(salicylidene)-1.2-propanediamine; D012545:Schiff Bases; D010984:Platinum; D002945:Cisplatin