title: Electro-transfer of small interfering RNA ameliorated arthritis in rats.
abstract: RNA interference provides the powerful means of sequence-specific gene silencing. Particularly, small interfering RNA (siRNA) duplexes may be potentially useful for therapeutic molecular targeting of human diseases, although novel delivery systems should be devised to achieve efficient and organ-specific transduction of siRNA. In the present study, we demonstrated that electro-transfer of a siRNA-polyamine complex made efficient and specific gene knockdown possible in the articular synovium. Targeted suppression of the tumor necrosis factor-alpha gene through this procedure significantly ameliorated collagen-induced arthritis in rats. Our results suggest the potential feasibility of therapeutic intervention with RNA medicines for treatment of rheumatoid and other locomotor diseases.
authors.affiliation: Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Inoue
authors.forename: Kenji A
authors.initials: KA
authors.lastname: Takahashi
authors.forename: Osam
authors.initials: O
authors.lastname: Mazda
authors.forename: Ryu
authors.initials: R
authors.lastname: Terauchi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Arai
authors.forename: Tsunao
authors.initials: T
authors.lastname: Kishida
authors.forename: Masaharu
authors.initials: M
authors.lastname: Shin-Ya
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Asada
authors.forename: Toru
authors.initials: T
authors.lastname: Morihara
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Tonomura
authors.forename: Suzuyo
authors.initials: S
authors.lastname: Ohashi
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Kajikawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawahito
authors.forename: Jiro
authors.initials: J
authors.lastname: Imanishi
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kawata
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Kubo
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D018274:Electroporation; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007719:Knee Joint / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D051381:Rats; D013583:Synovial Membrane / Q000378:metabolism; D014161:Transduction, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha; D049452:Green Fluorescent Proteins