title: Mutational analysis of the BRAF gene in human congenital and dysplastic melanocytic naevi.
abstract: Eighteen congenital melanocytic naevi (CMN) from 17 patients and 18 dysplastic melanocytic naevi (DMN) from 18 patients were screened for mutations in the BRAF oncogene (present study) and the N-ras oncogene (in the course of two foregoing studies) by single-strand conformational polymorphism (SSCP)/sequencing analysis. BRAF mutations were demonstrated in both types of lesion. As a whole, 17 of 18 CMN (94.4%) and five of 18 DMN (27.7%) harboured either BRAF or N-ras mutations. As the BRAF oncogene is frequently found to be mutated in human cutaneous melanomas, it may constitute a risk factor for melanoma formation within CMN and DMN.
authors.affiliation: Department of Biological Sciences, Institute of Cell Biology and Biosystem Technology, Rostock, Germany. thilo.papp@biologie.uni-rostock.de
authors.forename: Thilo
authors.initials: T
authors.lastname: Papp
authors.forename: Holger
authors.initials: H
authors.lastname: Schipper
authors.forename: Krishan
authors.initials: K
authors.lastname: Kumar
authors.forename: Dietmar
authors.initials: D
authors.lastname: Schiffmann
authors.forename: Regina
authors.initials: R
authors.lastname: Zimmermann
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009508:Nevus, Pigmented / Q000151:congenital* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D012878:Skin Neoplasms / Q000151:congenital* / Q000235:genetics*
chemical_list: C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf