title: Regulation of proliferation, motility, and contractivity of cultured human endometrial stromal cells by transforming growth factor-beta isoforms.
abstract: OBJECTIVE
  To evaluate the involvement of transforming growth factor (TGF)-beta isoforms (TGF-beta1, TGF-beta2, and TGF-beta3) on endometrial tissue remodeling during the perimenstrual period.
  
  
  METHODS
  The effects of TGF-beta isoforms on the cell proliferation, motility, and contractivity of cultured human endometrial stromal cells (ESCs) were investigated.
  
  
  METHODS
  Research laboratory at a medical school.
  
  
  METHODS
  Nine endometrial specimens in the late secretory phase were used.
  
  
  METHODS
  Endometrial stromal cells were incubated with recombinant human recombinant TGF-beta1, TGF-beta2, and TGF-beta3.
  
  
  METHODS
  The cell proliferation, motility, and contractivity of ESCs were accessed by a modified methylthiazoletetrazolium assay, in vitro wound repair assay, transwell invasion assay, and collagen gel contraction assay.
  
  
  RESULTS
  All three isoforms of TGF-beta significantly inhibited the cell proliferation of ESCs in a dose-dependent manner. In vitro wound repair assay and transwell invasion assay demonstrated that the TGF-beta isoforms significantly inhibited the motility of ESCs. However, the TGF-beta isoforms were shown to have a clear effect on the collagen gel contractivity of ESCs.
  
  
  CONCLUSIONS
  These results suggest that TGF-beta isoforms may promote endometrial tissue repair through the inhibition of the proliferation, expansion, and migration of ESCs, and through the stimulation of the contraction of the collagen gel matrix by these cells. Transforming growth factor-beta may be involved in the protection of the endometrium from extensive fibrosis and scarring by regulating ESC function during the perimenstrual period.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Oita, Japan. nasu@med.oita-u.ac.jp
authors.forename: Kaei
authors.initials: K
authors.lastname: Nasu
authors.forename: Masakazu
authors.initials: M
authors.lastname: Nishida
authors.forename: Harunobu
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Sun
authors.initials: S
authors.lastname: Bing
authors.forename: Chieko
authors.initials: C
authors.lastname: Inoue
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawano
authors.forename: Isao
authors.initials: I
authors.lastname: Miyakawa
mesh_terms: D000328:Adult; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D048429:Cell Size; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology* / Q000502:physiology*; D005260:Female; D006131:Growth Inhibitors / Q000502:physiology*; D006801:Humans; D020033:Protein Isoforms / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000502:physiology*; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2; D053782:Transforming Growth Factor beta3
chemical_list: D006131:Growth Inhibitors; D020033:Protein Isoforms; C508960:TGFB1 protein, human; C509041:TGFB2 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2; D053782:Transforming Growth Factor beta3