Two-step enzymatic synthesis of UDP-N-acetylgalactosamine.
UDP-GalNAc has been synthesised with high yield from GalNAc, UTP and ATP using recombinant human GalNAc kinase GK2 and UDP-GalNAc pyrophosphorylase AGX1. Both enzymes have been prepared in one step from 1L cultures of transformed Escherichia coli and the UDP-GalNAc produced has been purified by a simple procedure. The method described is a rapid and efficient means to produce UDP-GalNAc as well as analogues like UDP-N-azidoacetylgalactosamine (UDP-GalNAz).
Bourgeaux
Vanessa
V
Centre de Biophysique Moléculaire CNRS UPR4301 affiliée à l'Université d'Orléans et à l'INSERM, rue Charles Sadron, F-45071 Orléans Cedex 02, France.
Piller
Friedrich
F
Piller
Véronique
V
D000255:Adenosine Triphosphate / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology; D005700:Galactosyltransferases / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000096:biosynthesis*; D014544:Uridine Triphosphate / Q000378:metabolism
D017931:DNA Primers; D011994:Recombinant Proteins; D014536:Uridine Diphosphate N-Acetylgalactosamine; D000255:Adenosine Triphosphate; D005700:Galactosyltransferases; C511281:UDP-GalNAc pyrophosphorylase AGX1, human; C511279:GalNAc kinase GK2, pig; D017853:Phosphotransferases (Alcohol Group Acceptor); D014544:Uridine Triphosphate