title: A thermodynamic study on the interaction between magnesium ion and human growth hormone.
abstract: A thermodynamic study on the interaction between magnesium ion and human growth hormone (hGH) was studied at 27 degrees C in NaCl solution (50 mM) using different techniques. Two techniques of ionmetry using a Mg2+selective membrane electrode and isothermal titration calorimetry were applied to obtain the binding isotherm for hGHMg2+; results obtained by both techniques were found to be in good agreement. There is a set of three identical and noninteracting binding sites for magnesium ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 46 microM and -17.7 kJ/mol, respectively. Temperature scanning UV-visible spectroscopy was applied to elucidate the effect of Mg2+ binding on the protein stability, and circular dichroism (CD) spectroscopy was used to show the structural change of hGH due to the metal ion interaction. Magnesium ion binding increased the protein thermal stability by increasing the alpha-helix content as well as decreasing both beta and random coil structures. However, the secondary structural change of the protein returns to its native form, including a small change in the tertiary structure, in high concentrations of magnesium ion.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. saboury@ut.ac.ir
authors.forename: A A
authors.initials: AA
authors.lastname: Saboury
authors.forename: M S
authors.initials: MS
authors.lastname: Atri
authors.forename: M H
authors.initials: MH
authors.lastname: Sanati
authors.forename: A A
authors.initials: AA
authors.lastname: Moosavi-Movahedi
authors.forename: G H
authors.initials: GH
authors.lastname: Hakimelahi
authors.forename: M
authors.initials: M
authors.lastname: Sadeghi
mesh_terms: D002151:Calorimetry; D019382:Human Growth Hormone / Q000737:chemistry*; D006801:Humans; D017736:Ion-Selective Electrodes; D007477:Ions; D008274:Magnesium / Q000737:chemistry*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D013816:Thermodynamics
chemical_list: D007477:Ions; D019382:Human Growth Hormone; D008274:Magnesium