title: Study on the application of parent-of-origin specific DNA methylation markers to forensic genetics.
abstract: In paternity test, especially in motherless cases, the allele inherited from father (obligatory gene, OG) often cannot be determined. The paternity exclusion probability (PE) of a genetic marker is reduced considerably. Therefore, it is necessary to develop a new technique, by which the parental origin of alleles can be determined without genealogical analysis. In this paper, we explored the possibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles, choosing the imprinted single nucleotide polymorphism (SNP) locus rs220028 (A/G) as a model system. We typed the SNP by mutagenically separated PCR (MS-PCR). The frequencies of alleles were A = 0.5085, G = 0.4915; the unbiased heterozygosity was 0.5020. In order to discriminate between the maternal allele and paternal allele, post-digestion MS-PCR, a novel PCR based methylation analysis and SNP typing technique was developed and performed on 18 heterozygous children, and the methylated maternal allele was detected specifically. As a pilot study on the use of epigenetic markers in forensic genetics, our results demonstrated the feasibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles.
authors.affiliation: Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China. shenxiaomo2003@yahoo.com
authors.forename: Guisen
authors.initials: G
authors.lastname: Zhao
authors.forename: Qingen
authors.initials: Q
authors.lastname: Yang
authors.forename: Daixin
authors.initials: D
authors.lastname: Huang
authors.forename: Chunying
authors.initials: C
authors.lastname: Yu
authors.forename: Rongzhi
authors.initials: R
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Kun
authors.initials: K
authors.lastname: Mei
mesh_terms: D000483:Alleles; D002648:Child; D016172:DNA Fingerprinting / Q000379:methods*; D019175:DNA Methylation; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide
chemical_list: D005819:Genetic Markers