title: Single molecule adhesion measurements reveal two homophilic neural cell adhesion molecule bonds with mechanically distinct properties.
abstract: Neural cell adhesion molecule (NCAM) is a cell surface adhesion glycoprotein that plays an important role in the development and stability of nervous tissue. The homophilic binding mechanism of NCAM is still a subject of debate on account of findings that appear to support different mechanisms. This paper describes single molecule force measurements with both full-length NCAM and NCAM mutants that lack different immunoglobulin (Ig) domains. By systematically applying an external, time-dependent force to the bond, we obtained parameters that describe the energy landscape of NCAM-NCAM bonds. Histograms of the rupture forces between the full-length NCAM extracellular domains revealed two binding events, one rupturing at higher forces than the other. These bond rupture data show that the two bonds have the same dissociation rates. Despite the energetic and kinetic similarities, the bond strengths differ significantly, and are mechanically distinct. Measurements with NCAM domain deletion mutants mapped the weaker bond to the Ig1-2 segment, and the stronger bond to the Ig3 domain. Finally, the quantitative agreement between the fragment adhesion and the strengths of both NCAM bonds shows that the domain deletions considered in this study do not alter the intrinsic strengths of either of the two bonds.
authors.affiliation: Department of Chemistry, University of Illinois, Urbana, Illinois 61801, USA.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Wieland
authors.forename: Andrew A
authors.initials: AA
authors.lastname: Gewirth
authors.forename: Deborah E
authors.initials: DE
authors.lastname: Leckband
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002645:Chickens; D002942:Circular Dichroism; D017353:Gene Deletion; D007136:Immunoglobulins / Q000737:chemistry; D008969:Molecular Sequence Data; D009154:Mutation; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry* / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D007136:Immunoglobulins; D019006:Neural Cell Adhesion Molecules; D010446:Peptide Fragments