[The genes change of hMSC before and after the differentation into neuron-like cells].
OBJECTIVE
To study the genes change of hMSC before and after differentiation into neuron-like cells.


METHODS
hMSC were separated from marrow, cultured and expanded in culture medium. After hMSC being induced to differentiate with Shenqiye, Neuron-specific enolase (MSE), neurofilament (NF), and glial fibrillary acidic protein (GFAP) were detected by immunocytochemistry respectively. Using semi-quantitiative RT-PCR to detect ten genes change in hMSC before and after differentiation. Two genes were used to detect the gene sequence.


RESULTS
When treated with the inducer--Shenqiye, hMSC exhibited neuronal phenotype. The expressions of NSE and NF in the neuron-like cells were positive, but the glial astrocyte marker GFAP was not found. hMSC expressed germline, ectodermal, endodermal and mesodermal genes prior to neurogenasis. After differentiation, germline genes did not express, while the expressions of endodermal and mesodermal genes were weakened. Ectodermal and neuronal genes expressed stronger.


CONCLUSIONS
hMSC express multipotentiality, and the neural differentiation comprises quantitative modulation of gene expression rather than simple on-off switching of neural specific genes.
Yao
Xiaoli
X
Department of Neurology, The First Affiliated Hospital, Guangzhou.
Zhang
Cheng
C
Feng
Shanwei
S
Lu
Xilin
X
Liu
Zuguo
Z
Deng
Yubin
Y
D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015870:Gene Expression; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D016900:Neurofilament Proteins / Q000096:biosynthesis / Q000235:genetics; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins