title: Correlation between plasma leptin concentration and body fat content in dogs.
abstract: OBJECTIVE
  To evaluate the relationship between plasma leptin concentration and body fat content in dogs.
  
  
  METHODS
  20 spayed female Beagles that were 10 months old at the start of the experiment.
  
  
  METHODS
  Dogs were kept under regulated feeding and exercise conditions for 21 weeks, resulting in a wide range of body weights, body condition scores (BCS), and subcutaneous thicknesses. Plasma leptin concentration was measured by use of a canine leptin-specific ELISA test to evaluate its correlation to body fat content estimated by the deuterium oxide dilution method. Plasma concentrations of glucose, cholesterol, triglycerides (TG), and nonesterified fatty acids (NEFA) were also measured.
  
  
  RESULTS
  Body fat content (9 to 60% of body weight) was positively and closely correlated (r = 0.920; n = 20; P < 0.001) to plasma leptin concentration (0.67 to 8.06 ng/ml), compared with other variables (ie, glucose, cholesterol, TG, and NEFA; r = 0.142, 0.412, 0.074, and 0.182, respectively).
  
  
  CONCLUSIONS
  The positive relationship between plasma leptin concentration and body fat content in dogs was similar to correlations reported for humans and rodents, suggesting that plasma leptin is a quantitative marker of adiposity in dogs.
authors.affiliation: Research Center, Nippon Pet Food Co, Ltd, 2020 Umeyama, Asaba, Shizuoka 437-1105, Japan.
authors.forename: Mayumi M
authors.initials: MM
authors.lastname: Sagawa
authors.forename: Fumio
authors.initials: F
authors.lastname: Nakadomo
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Honjoh
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ishioka
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saito
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology*; D000821:Animal Feed; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001835:Body Weight; D004283:Dog Diseases / Q000097:blood; D004285:Dogs / Q000033:anatomy & histology* / Q000097:blood*; D005260:Female; D020738:Leptin / Q000097:blood*; D009765:Obesity / Q000097:blood / Q000662:veterinary
chemical_list: D015415:Biomarkers; D020738:Leptin