title: The NC1 dimer of human placental basement membrane collagen IV: does a covalent crosslink exist?
abstract: Triple-helical collagen IV protomers associate through their N- and C-termini, forming a three-dimensional network that provides basement membranes with mechanical strength. Within this network, the C-terminal non-collagenous (NC1) domains form tight dimeric junctions. Crystallographic analyses of isolated NC1 domains show two trimeric cap-like structures interacting via a large interface. Previously, for NC1 from human placenta type-IV collagen we described covalent alpha1-alpha1 and alpha2-alpha2 crosslinks between Met93 and Lys211 of opposing alpha1(IV) and alpha2(IV) NC1-chains, which further stabilize this interface and explain the occurrence of reduction-insensitive NC1-chain dimers. However, their existence was recently questioned, and we therefore analyzed NC1-domain dimers in more detail by biochemical and protein crystallographic methods. Short-exposure diffraction data show a clear electron density cross-connecting the respective residues, which gradually disappears with prolonged crystal irradiation. Sequence analyses of isolated tryptic peptides derived from denatured NC1 monomers and dimers indicate that only the dimers, but not the monomers, yield these chemically labile cross-linked peptides. These data clearly demonstrate the presence of reduction-resistant, but chemically and radiation-sensitive covalent crosslinks between the side chains of Met93 and Lys211 in human placenta type-IV collagen.
authors.affiliation: Max-Planck-Institut für Biochemie, Am Klopferspitz 18, D-82152 Martinsried, Germany.
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Than
authors.forename: Gleb P
authors.initials: GP
authors.lastname: Bourenkov
authors.forename: Stefan
authors.initials: S
authors.lastname: Henrich
authors.forename: Karlheinz
authors.initials: K
authors.lastname: Mann
authors.forename: Wolfram
authors.initials: W
authors.lastname: Bode
mesh_terms: D000595:Amino Acid Sequence; D001485:Basement Membrane / Q000378:metabolism*; D024141:Collagen Type IV / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D019281:Dimerization; D005260:Female; D006801:Humans; D008239:Lysine / Q000737:chemistry; D008715:Methionine / Q000737:chemistry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D014357:Trypsin / Q000378:metabolism
chemical_list: D024141:Collagen Type IV; D003432:Cross-Linking Reagents; D010446:Peptide Fragments; D008715:Methionine; D014357:Trypsin; D008239:Lysine