title: [The changes of IGF-I in testis and epididymis on a rat model with oligozoospermia/azoospermia induced by cyclophosphamide].
abstract: OBJECTIVE
  To evaluate the effect of the levels of IGF-I in the epididymis and the expression of IGF-I in the testis of adult male rat after the administration of cyclophosphamide.
  
  
  METHODS
  Ninety-six male adult rats (8 weeks age) were divided into 6 groups. The doses given to the rats of the groups 1 to 5 were 10, 20, 40, 80 and 100 mg/(kg x d), respectively. The remaining group was served as control. All those rats were sacrificed and IGF-I were quantitatively determined by ELISA techniques 2 and 4 weeks after the administration of the drug (by gastric fudge). Immunohistochemical SP technique was used to examine expression of IGF-I in rat testis.
  
  
  RESULTS
  The levels of cell factors (IGF-I) in the epididymis of the rats were gradually reduced with the increasing time and dose after administration of the drug. In the mean time the expression of IGF-I in the tissues of the testis of those rats were also gradually reduced.
  
  
  CONCLUSIONS
  In the time of oligozoospermia/azoospermia induced by the administration of cyclophosphamide, the expression levels of IGF-I in the genetic system were significantly reduced. The possible mechanism of these changes could be attributed to the lower spermatogenesis function of the testis caused by the administration of cyclophosphamide.
authors.affiliation: Department of Urology, Wuhan General Hospital of Guanzhou Command, PLA, Wuhan, Hubei 430070, China. whsgq@sina.com
authors.forename: Guo-qiu
authors.initials: GQ
authors.lastname: Shen
authors.forename: Gong-cheng
authors.initials: GC
authors.lastname: Lu
authors.forename: Tie-jun
authors.initials: TJ
authors.lastname: Pan
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Xiao
mesh_terms: D000818:Animals; D053713:Azoospermia / Q000139:chemically induced / Q000378:metabolism*; D003520:Cyclophosphamide / Q000633:toxicity*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004822:Epididymis / Q000378:metabolism*; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D008297:Male; D009845:Oligospermia / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000378:metabolism*
chemical_list: D007334:Insulin-Like Growth Factor I; D003520:Cyclophosphamide