Ca2+/calmodulin protein kinase II and memory: learning-related changes in a localized region of the domestic chick brain.
The role of calcium/calmodulin-dependent protein kinase II (CaMKII) in the recognition memory of visual imprinting was investigated. Domestic chicks were exposed to a training stimulus and learning strength measured. Trained chicks, together with untrained chicks, were killed either 1 h or 24 h after training. The intermediate and medial hyperstriatum ventrale/mesopallium (IMHV/IMM), a forebrain memory storage site, was removed together with a control brain region, the posterior pole of the neostriatum/nidopallium (PPN). Amounts of membrane total alphaCaMKII (tCaMKII) and Thr286-autophosphorylated alphaCaMKII (apCAMKII) were measured. For the IMHV/IMM 1 h group, apCaMKII amount and apCAMKII/tCaMKII increased as chicks learned. The magnitude of the molecular changes were positively correlated with learning strength. No learning-related effects were observed in PPN, or in either region at 24 h. These results suggest that CaMKII is involved in the formation of memory but not in its maintenance.
Solomonia
Revaz O
RO
Institute of Physiology, Georgian Academy of Sciences, Tbilisi, Republic of Georgia.
Kotorashvili
Adam
A
Kiguradze
Tamar
T
McCabe
Brian J
BJ
Horn
Gabriel
G
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D001522:Behavior, Animal / Q000502:physiology; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000032:analysis / Q000502:physiology*; D002645:Chickens; D003627:Data Interpretation, Statistical; D004586:Electrophoresis; D007150:Immunohistochemistry; D007858:Learning / Q000502:physiology*; D008568:Memory / Q000502:physiology*; D017072:Neostriatum / Q000201:enzymology / Q000502:physiology; D010766:Phosphorylation; D016548:Prosencephalon / Q000201:enzymology* / Q000502:physiology*; D013997:Time Factors
D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases