Specific binding of dacarbazine to DNA bases and oligonucleotides based on gold nanoparticles.
We studied the specific binding of an anticancer drug, dacarbazine (DTIC), to DNA bases and oligonucleotides attached to gold nanoparticles by using electrochemical methods, and the results indicate that the presence of gold nanoparticles could facilitate the binding of dacarbazine to specific DNA bases and remarkably enhance the relative detection sensitivity. The results of the study on interaction of dacarbazine with oligonucleotides also illustrate that dacarbazine could recognize some specific sequence in DNA chain and sensitively detect single-base mismatch in DNA helix.
Zhang
Renyun
R
Chien-Shiung Wu Laboratory, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P R. China.
Wang
Xuemei
X
He
Nongyue
N
D018906:Antineoplastic Agents, Alkylating / Q000378:metabolism*; D020137:Base Pair Mismatch; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003606:Dacarbazine / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D006046:Gold; D066298:In Vitro Techniques; D015335:Molecular Probes; D049329:Nanostructures; D036103:Nanotechnology; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D018906:Antineoplastic Agents, Alkylating; D015335:Molecular Probes; D009838:Oligodeoxyribonucleotides; D006046:Gold; D003606:Dacarbazine; D004247:DNA