title: Characterization of mesenchymal stem cells isolated from mouse fetal bone marrow.
abstract: Mesenchymal stem cells (MSCs) are defined as cells that can differentiate into multiple mesenchymal lineage cells. MSCs have some features (surface molecules and cytokine production, etc.) common to so-called traditional bone marrow (BM) stromal cells, which have the capacity to support hemopoiesis. In the present study, we isolated murine MSCs (mMSCs) from the fetal BM using an anti-PA6 monoclonal antibody (mAb) that is specific for bone marrow stromal cells. The mMSCs, called FMS/PA6-P cells, are adherent, fibroblastic, and extensively expanded and have the ability to differentiate not only into osteoblasts and adipocytes but also into vascular endothelial cells. The FMS/PA6-P cells produce a broad spectrum of cytokines and growth factors closely related to hemopoiesis and show good hemopoiesis-supporting capacity both in vivo and in vitro, suggesting that they are a component of the hemopoietic stem cell niche in vivo. Interestingly, although the FMS/PA6-P cells express a high level of the PA6 molecule, which is reactive with anti-PA6 mAb, they gradually lose their ability to express this molecule during the course of differentiation into osteoblasts and adipocytes, indicating that the PA6 molecule might serve as a novel marker of mMSCs.
authors.affiliation: First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi City, Osaka 570-8506, Japan.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hisha
authors.forename: Shigeru
authors.initials: S
authors.lastname: Taketani
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Adachi
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Wenhao
authors.initials: W
authors.lastname: Cui
authors.forename: Yunze
authors.initials: Y
authors.lastname: Cui
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Changye
authors.initials: C
authors.lastname: Song
authors.forename: Tomomi
authors.initials: T
authors.lastname: Mizokami
authors.forename: Satoshi
authors.initials: S
authors.lastname: Okazaki
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Tianxue
authors.initials: T
authors.lastname: Fan
authors.forename: Hongxue
authors.initials: H
authors.lastname: Fan
authors.forename: Zhexiong
authors.initials: Z
authors.lastname: Lian
authors.forename: M Eric
authors.initials: ME
authors.lastname: Gershwin
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D005333:Fetus / Q000166:cytology / Q000502:physiology*; D006133:Growth Substances / Q000096:biosynthesis; D006410:Hematopoiesis / Q000502:physiology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology
chemical_list: D000943:Antigens, Differentiation; D006133:Growth Substances