title: Application of an electric DNA-chip for the expression analysis of bioprocess-relevant marker genes of Bacillus subtilis.
abstract: The knowledge of critical process-relevant genes can be used for an improved control of bioprocesses. So far bioprocess-relevant marker genes can be analyzed by established expression analysis methods only off-line. In this study, an alternative approach for a potential at-line monitoring of gene expression during bioprocesses is suggested. This approach is based on the measurement of specific mRNAs on an electric DNA-chip in connection with a magnetic bead-based sandwich hybridization. In order to allow an at-line measurement of specific mRNAs an improved method for a fast and partially automated isolation of high quality-RNA samples was developed. The expression analysis of the electric DNA-chip was compared with optical DNA micro arrays and the real time RT-PCR for three selected process-relevant genes of Bacillus subtilis. We demonstrate that the mRNA analysis by means of the electric DNA-chip gives similar results compared to the micro array analysis and the real time RT-PCR technique.
authors.affiliation: Institute of Microbiology, Ernst-Moritz-Arndt-University Greifswald, F.-L.-Jahnstrasse 15, D-17487 Greifswald, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Jürgen
authors.forename: Kim Bundvig
authors.initials: KB
authors.lastname: Barken
authors.forename: Steffen
authors.initials: S
authors.lastname: Tobisch
authors.forename: Daniel
authors.initials: D
authors.lastname: Pioch
authors.forename: Mogens
authors.initials: M
authors.lastname: Wümpelmann
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
authors.forename: Thomas
authors.initials: T
authors.lastname: Schweder
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D015415:Biomarkers; D004563:Electrochemistry / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009862:Online Systems
chemical_list: D001426:Bacterial Proteins; D015415:Biomarkers