title: Efficient production, purification, and application of egg yolk antibodies against human HLA-A*0201 heavy chain and light chain (beta2m).
abstract: The importance of eggs as a source of specific antibodies is well recognized. Egg yolk contains 8--20mg immunoglobulins (IgY) per milliliter. However, the major problem in separating IgY is to remove the high concentrations of lipids in egg yolk. We first used water dilution method to get the supernatant containing IgY, then purified the antibody by caprylic acid-ammonium sulfate method, and obtained specific antibody with satisfactory purity and activity. By comparison of these several methods, each has its advantages, one can be chosen to purify IgY according to practical need. The purified IgY produced by the immunized chickens can stain the human peripheral blood mononuclear cell effectively when labeled with fluorescent FITC.
authors.affiliation: Institute of Molecular Immunology, Southern Medical University, Guangzhou 510515, PR China. ruangp2002@hotmail.com
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Ruan
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: He
authors.forename: Min-Jie
authors.initials: MJ
authors.lastname: Meng
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Ming-Qian
authors.initials: MQ
authors.lastname: Zhou
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Hu
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang
mesh_terms: D000818:Animals; D002645:Chickens / Q000276:immunology; D004530:Egg Yolk / Q000737:chemistry* / Q000276:immunology; D015234:HLA-A Antigens / Q000276:immunology; D015789:HLA-A2 Antigen; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D001613:beta 2-Microglobulin / Q000276:immunology
chemical_list: D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; C016512:IgY; D007136:Immunoglobulins; D001613:beta 2-Microglobulin