title: [Identification of protein peroxiredoxin 2 related to crystalline NiS-induced neoplastic transformation].
abstract: OBJECTIVE
  To provide evidence for illustrating the molecular mechanism of nickel carcinogenesis, and to identify the differential expression of protein in crystalline NiS-induced neoplastic transformation of human bronchial epithelial cell by proteomics technology.
  
  
  METHODS
  Two dimensional electrophoresis (2-DE) and the ImageMaster 3.10 software were used to analyze the differential expression of protein, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) combined with database search was applied to identify protein peroxiredoxin 2 (PDX2) related to malignant transformation.
  
  
  RESULTS
  The good 2-DE pattern including resolution and reproducibility was obtained. Nearly 700 expressed proteins per 2-D gel were isolated with molecular weights (MW) ranging from 14,400 to 94,000 KD and pI 3 - 10. A protein PDX2 with MW 21,890 KD, pI 5.66, which was highly expressed in malignantly transformed cell, was identified using MALDI-TOF-MS.
  
  
  CONCLUSIONS
  PDX2 was involved in malignant transformation of human bronchial epithelial cell induced by crystalline nickel sulfide.
authors.affiliation: Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou 510182, China.
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Ji
authors.forename: Jia-kun
authors.initials: JK
authors.lastname: Chen
authors.forename: Jia-chun
authors.initials: JC
authors.lastname: Lü
authors.forename: Zhong-liang
authors.initials: ZL
authors.lastname: Wu
authors.forename: Su-mei
authors.initials: SM
authors.lastname: Feng
authors.forename: Fei
authors.initials: F
authors.lastname: Yi
mesh_terms: D001980:Bronchi / Q000166:cytology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D009532:Nickel / Q000633:toxicity*; D054464:Peroxiredoxins / Q000378:metabolism*; D020543:Proteome
chemical_list: D020543:Proteome; D009532:Nickel; C086065:PRDX2 protein, human; D054464:Peroxiredoxins; C017558:nickel sulfide