title: [Expression and clinical significance of decorin in stress urinary incontinence].
abstract: OBJECTIVE
  To observe the relation between content of type III collagen and expression of decorin in paraurethral connective tissue in patients with stress urinary incontinence (SUI).
  
  
  METHODS
  Transvaginal biopsies were obtained from the paraurethral connective tissue in 43 SUI and non-SUI control women. The concentration of type III collagen was determined by immunohistochemical technique, electrophoretic separation and quantification. RT-PCR was used to verify the mRNA level of decorin.
  
  
  RESULTS
  The mean concentration of type III collagen was significantly reduced in patients with SUI (23 +/- 4), compared to the control group (34 +/- 6, P < 0.05). The expression of decorin was significantly higher in patients with SUI (1.17 +/- 0.18), compared to the control group (0.76 +/- 0.16, P < 0.05), and it was highly and negatively related to the content of type III collagen (r = -0.720, P < 0.05).
  
  
  CONCLUSIONS
  Our study results suggest that collagen metabolism in SUI is altered by decorin. This alteration results in a less flexible connective tissue, which is probably more likely to break. Decorin might be involved in the pathogenesis of SUI.
authors.affiliation: Department of Obstetrics and Gynecology, Fuzhou General Hospital, Nanjing Military Command, Fuzhou 350025, China.
authors.forename: Yi-bin
authors.initials: YB
authors.lastname: Lin
authors.forename: Yan-feng
authors.initials: YF
authors.lastname: Song
mesh_terms: D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism*; D058575:Decorin / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014550:Urinary Incontinence, Stress / Q000378:metabolism* / Q000473:pathology
chemical_list: D024061:Collagen Type III; D058575:Decorin; D011509:Proteoglycans; D012333:RNA, Messenger