A novel method for immediate post-purification purity determination of fractions collected during high-throughput purification.
Following purification, the fractions of purified samples typically are analyzed to determine the relative purities of each fraction. We report a novel technique for performing post-purification analysis immediately after each preparative LC/MS run. The Single Pass Compound Purification and Analysis System (SPACPASS) samples and stores a representative aliquot from the fraction while it is being collected. Demonstrated for '1:1' fraction collections, this method of fraction purity assessment streamlined sample processing by reducing post-purification sample handling. For 97% of the collected fractions, this technique provided relative purities to within +/-5% when compared with more traditional post-purification analysis.
Yuan
Ding
D
Department of Analytical and Bioanalytical Chemistry, Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA.
Petersen
Chris
C
Rynd
Matthew
M
Mainquist
James
J
Meyer
Andrew
A
Isbell
John J
JJ
D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet