The effect of selected aromatic bromine derivatives on the activity of glutathione peroxidase and transferase.
OBJECTIVE
Glutathione (GSH) is an important element of antioxidative barrier. Its biological function consists in eliminating oxygen free radicals. It also acts as a co-substrate in numerous enzymatic reactions catalyzed by glutathione peroxidase (GPx) and glutathione S-transferase (GST). In our study we attempted to assess the effect of hexabromobenzene (HBB) and its metabolites on the level of GSH and related enzymes, GPx and GST.


METHODS
The experiments were performed on female Wistar rats. The investigated compounds (HBB, 1,2,4,5-tetraBB, 1,2,4- and 1,3,5-triBB) were administered intragastrically in three different doses (HBB: 15, 75, and 375 mg/kg; 1,2,4,5-tetraBB and 1,2,4-triBB: 8, 40, and 200 mg/kg; 1,3,5-triBB: 12, 60, and 300 mg/kg) for 7, 14, 21 or 28 days. GSH level and activity of GST and GPx were determined in the obtained material.


RESULTS
The highest activity of GPx and GSTwas observed after a 7-fold administration of all investigated compounds. Prolonged time of exposure caused the return to the control values.


CONCLUSIONS
The study revealed that repeated exposure to aromatic bromine derivatives increases GPx and GST activity only in the initial phase of the experiment.
Frydrych
Barbara
B
Depertment of Toxicology, Faculty of Pharmacy, Medical University, Lódź, Poland. jszymanska@pharm.am.lodz.pl
Szymańska
Jadwiga A
JA
Sala
Monika
M
Bruchajzer
Elzbieta
E
D000818:Animals; D001966:Bromine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001969:Bromobenzenes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D005411:Flame Retardants / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005979:Glutathione Peroxidase / Q000097:blood* / Q000187:drug effects; D005982:Glutathione Transferase / Q000097:blood* / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D001969:Bromobenzenes; D005411:Flame Retardants; C110818:1,2,4,5-tetrabromobenzene; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D001966:Bromine; C017363:hexabromobenzene