Global gene expression profile of human cord blood-derived CD133+ cells.
Human cord blood (CB)-derived CD133+ cells carry characteristics of primitive hematopoietic cells and proffer an alternative for CD34+ cells in hematopoietic stem cell (HSC) transplantation. To characterize the CD133+ cell population on a genetic level, a global expression analysis of CD133+ cells was performed using oligonucleotide microarrays. CD133+ cells were purified from four fresh CB units by immunomagnetic selection. All four CD133+ samples showed significant similarity in their gene expression pattern, whereas they differed clearly from the CD133- control samples. In all, 690 transcripts were differentially expressed between CD133+ and CD133- cells. Of these, 393 were increased and 297 were decreased in CD133+ cells. The highest overexpression was noted in genes associated with metabolism, cellular physiological processes, cell communication, and development. A set of 257 transcripts expressed solely in the CD133+ cell population was identified. Colony-forming unit (CFU) assay was used to detect the clonal progeny of precursors present in the studied cell populations. The results demonstrate that CD133+ cells express primitive markers and possess clonogenic progenitor capacity. This study provides a gene expression profile for human CD133+ cells. It presents a set of genes that may be used to unravel the properties of the CD133+ cell population, assumed to be highly enriched in HSCs.
Jaatinen
Taina
T
Finnish Red Cross Blood Service, Kivihaantie 7, 00310 Helsinki, Finland.
Hemmoranta
Heidi
H
Hautaniemi
Sampsa
S
Niemi
Jari
J
Nicorici
Daniel
D
Laine
Jarmo
J
Yli-Harja
Olli
O
Partanen
Jukka
J
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay / Q000379:methods; D005312:Fetal Blood / Q000166:cytology / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology*; D006023:Glycoproteins; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010455:Peptides
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides