title: [Field validation of a real-time PCR test for the detection of Mycoplasma hyopneumoniae in nasal swabs of live pigs].
abstract: Enzootic pneumonia (EP) of pigs, caused by Mycoplasma hyopneumoniae has been a notifiable disease in Switzerland since May 2003. The diagnosis of EP has been based on multiple methods, including clinical, bacteriological and epidemiological findings as well as pathological examination of lungs (mosaic diagnosis). With the recent development of a real-time PCR (rtPCR) assay with 2 target sequences a new detection method for M. hyopneumoniae became available. This assay was tested for its applicability to nasal swab material from live animals. Pigs from 74 herds (average 10 pigs per herd) were tested. Using the mosaic diagnosis, 22 herds were classified as EP positive and 52 as EP negative. From the 730 collected swab samples we were able to demonstrate that the rtPCR test was 100% specific. In cases of cough the sensitivity on herd level of the rtPCR is 100%. On single animal level and in herds without cough the sensitivity was lower. In such cases, only a positive result would be proof for an infection with M. hyopneumoniae. Our study shows that the rtPCR on nasal swabs from live pigs allows a fast and accurate diagnosis in cases of suspected EP.
authors.affiliation: Departement für klinische Veterinärmedizin, Abteilung für Schweinemedizin, Universität Bern, Schweiz. friederike.zeeh@knp.unibe.ch
authors.forename: F
authors.initials: F
authors.lastname: Zeeh
authors.forename: P
authors.initials: P
authors.lastname: Kuhnert
authors.forename: R
authors.initials: R
authors.lastname: Miserez
authors.forename: M G
authors.initials: MG
authors.lastname: Doherr
authors.forename: W
authors.initials: W
authors.lastname: Zimmermann
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D045705:Mycoplasma hyopneumoniae / Q000302:isolation & purification*; D009297:Nasal Mucosa / Q000382:microbiology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000175:diagnosis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D004269:DNA, Bacterial