Magnetically actuated micromixing on an array-pattern microfluidic chip for immunoassay of human thyrotropin.
A novel array-pattern ring microfluidic chip has been designed and fabricated with ITO glass, and a low-cost, easy-to-use active magnetic mixer is developed. The mixer consists of externally driven magnetic stir plates and ferromagnetic microneedles as active mobile stirrers in the microchannel. Mixing performance in the microfluidic chip has been experimentally characterized by chemiluminescence immunoassay of human thyrotropin (TSH) and compared with tube-based chemiluminescence immunoassay. The mixing method proposed in this study can be readily applied to integrated microfluidic systems, such as micro-total-analysis systems, lab-on-a-chip, and so on.
Li
Chengwu
C
The Center of Advanced Microfabrication and Integration Technology, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P R. China.
Wang
Yongning
Y
Gao
Yunhua
Y
Guo
XiaoYing
X
Gu
Zhongze
Z
D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D008163:Luminescent Measurements; D008280:Magnetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology; D013972:Thyrotropin / Q000032:analysis*
D013972:Thyrotropin