Microstructural and strength evaluation of regenerate tissue during the consolidation period after vertical mandibular ramus distraction.
Mandibular ramus height restoration by distraction osteogenesis (DO) is a key procedure in mandibular hypoplasia reconstruction. The objective of this study was to evaluate short-term skeletal changes in the regenerated bone after vertical mandibular ramus DO using a buried distraction device. Eight subadult beagle dogs underwent bilateral vertical mandibular ramus DO. After a 7-day latency period, distraction was performed at a rate of 0.5 mm twice a day for 12 days. Four dogs were killed at 1 month and four dogs at 2 months after the end of distraction. One intact beagle was included as an unoperated control. After sacrifice, micro computed tomography (muCT) and mechanical testing of distracted sites were used to measure bone volume (BV), total volume (TV), and mechanical peak load strength, respectively. The muCT images showed wide variation in the response, with some animals demonstrating considerable bone formation and reconstitution of the canal for the inferior alveolar nerve. Quantitatively, BV was no more than 67% and BV/TV was less than 25% of the intact control, and strength was approximately 33% of the intact control value. The 1 and 2 month values were similar. These results suggest that internal distractors can successfully reconstitute bone but that the regenerated tissue did not regain structural and mechanical characteristics of native bone within the 2 month study period.
Gomez
David F
DF
Rush Craniofacial Center, Department of Plastic and Reconstructive Surgery, Rush University Medical Center, Chicago, Illinois 60612, USA.
Sant'Anna
Eduardo F
EF
Leven
Robert M
RM
Ostric
Sjdran A
SA
Figueroa
Alvaro A
AA
Royston
Thomas J
TJ
Sumner
Dale R
DR
Polley
John W
JW
D000818:Animals; D015519:Bone Density / Q000502:physiology; D001861:Bone Regeneration / Q000502:physiology*; D002113:Calcification, Physiologic / Q000502:physiology; D004285:Dogs; D016268:Internal Fixators; D008297:Male; D008334:Mandible / Q000502:physiology / Q000601:surgery* / Q000648:ultrastructure; D008340:Mandibular Nerve / Q000033:anatomy & histology; D008852:Microradiography; D023421:Models, Animal; D010012:Osteogenesis / Q000502:physiology; D019857:Osteogenesis, Distraction / Q000295:instrumentation; D013314:Stress, Mechanical; D013997:Time Factors; D014057:Tomography, X-Ray Computed / Q000379:methods