parC mutations in fluoroquinolone-resistant Borrelia burgdorferi.
We have isolated in vitro fluoroquinolone-resistant mutants of the Lyme disease agent, Borrelia burgdorferi. Mutations in parC, which encodes a subunit of topoisomerase IV, were associated with loss of susceptibility to sparfloxacin, moxifloxacin, and Bay-Y3118, but not ciprofloxacin. This is the first description of fluoroquinolone resistance in the spirochete phylum.
Galbraith
Kendal M
KM
Division of Biological Sciences, The University of Montana, Missoula, 59812-4824, USA.
Ng
Amanda C
AC
Eggers
Betsy J
BJ
Kuchel
Craig R
CR
Eggers
Christian H
CH
Samuels
D Scott
DS
D000890:Anti-Infective Agents / Q000494:pharmacology*; D025065:Borrelia burgdorferi / Q000235:genetics*; D004269:DNA, Bacterial; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones / Q000494:pharmacology*; D005798:Genes, Bacterial; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA
D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D024841:Fluoroquinolones