title: Low pH changes the profile of nodulation factors produced by Rhizobium tropici CIAT899.
abstract: Rhizobium tropici CIAT899 has been cataloged as a nodulator of bean, a plant often growing in areas characterized by highly acidic soils. The purpose of this work was to explore the effects of acidity on the production of Nod factors by this strain and their impact on the establishment of effective symbioses. We report that acidity increases rhizobial Nod factors production, and we exhaustively study the nodulation factor structures produced under abiotic stress. Significant differences were observed between the structures produced at acid and neutral pH: 52 different molecules were produced at acid pH, 29 at neutral pH, and only 15 are common to bacteria grown at pH 7.0 or 4.5. The results indicate that R. tropici CIAT899 has successfully adapted to life in acidic soils and is a good inoculant for the bean under these conditions.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, c/ Profesor García González, Spain.
authors.forename: Belén
authors.initials: B
authors.lastname: Morón
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Soria-Díaz
authors.forename: James
authors.initials: J
authors.lastname: Ault
authors.forename: George
authors.initials: G
authors.lastname: Verroios
authors.forename: Sadaf
authors.initials: S
authors.lastname: Noreen
authors.forename: Dulce N
authors.initials: DN
authors.lastname: Rodríguez-Navarro
authors.forename: Antonio
authors.initials: A
authors.lastname: Gil-Serrano
authors.forename: Jane
authors.initials: J
authors.lastname: Thomas-Oates
authors.forename: Manuel
authors.initials: M
authors.lastname: Megías
authors.forename: Carolina
authors.initials: C
authors.lastname: Sousa
mesh_terms: D000222:Adaptation, Physiological; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D013058:Mass Spectrometry; D008745:Methylation; D009586:Nitrogen Fixation / Q000235:genetics; D027805:Phaseolus / Q000254:growth & development / Q000382:microbiology; D042162:Rhizobium tropici / Q000378:metabolism* / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides