title: Development of efficient packaging method of oligodeoxynucleotides by a condensed nano particle in lipid envelope structure.
abstract: An efficient delivery system is required if antisense oligodeoxynucleotides (ODN) are to be utilized for gene therapy. We report herein on the development of a novel ODN delivery system, ODN-encapsulated nano particles (ODN-ENP) using an efficient and simple packaging method. The ODN-ENP consists of a condensed ODN particle and a lipid envelope, which can be equipped with various functional devices for the efficient delivery of ODN with a small diameter (150 nm). The encapsulation efficiency and ODN recovery of ODN-ENP were significantly higher than those of other packaging methods, such as a stabilized antisense-lipid particles method or a freeze-thaw method. Furthermore, the time required for the preparation of the ODN-ENP was shorter than the other methods. The method developed in this study is a simple and efficient packaging method for ODN with a condensed nano particle in lipid-envelope structure.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University; Kita-12, Nishi-6, Sapporo 060-0812, Japan.
authors.forename: Yuma
authors.initials: Y
authors.lastname: Yamada
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kogure
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Kaori
authors.initials: K
authors.lastname: Inoue
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Akita
authors.forename: Fumi
authors.initials: F
authors.lastname: Nagatsugi
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sasaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Suhara
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D001483:Base Sequence; D004337:Drug Carriers; D005615:Freezing; D008055:Lipids / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010316:Particle Size
chemical_list: D004337:Drug Carriers; D008055:Lipids; D009838:Oligodeoxyribonucleotides