[A new rat model of cerebral infarction based on the injury of vascular endothelial cell].
OBJECTIVE
A new rat model of cerebral infarction was developed to elucidate the contribution of vascular endothelial cell during focal cerebral infarction formation.


METHODS
Forty-eight Sprague-Dawley (SD) rats were randomly divided into the model group, sham operation group, and control group for indexes observation of triphenyltetrazolium chloride (TTC) dyeing, neurological deficit, plasma tissue-type plasminogen activator (tPA) activity, plasminogen activator inhibitor (PAI) activity, thromboxane B(2) (TXB(2)) content, and 6-keto-prostaglandin (6-keto-PGF(1alpha)) content.


RESULTS
(1) The highest neurological score appeared at 6 h after operation, descending significantly at sequential time. (2) Using TTC dyeing and optical microscope technique, pathological changes in brains were observed. (3) Compared with control group and sham operation groups, there was a decrease in tPA activity of model rats at the initial 12 h after injection of sodium laurate (P < 0.05), PAI activity decreased markedly in the model group at 24 h after injection of sodium laurate. (4) In plasma TXB(2) concentration reached the highest level compared at 6 h after injection of sodium laurate, but there were not obvious differences in plasma 6-keto-PGF(1alpha) concentration among all groups (P > 0.05).


CONCLUSIONS
Focal cerebral infarction in rats could be induced by some sodium laurate, showing ischemic cerebrum necrosis, function disorder of vascular endothelium-platelet, fibrinolysis abnormality. This model could play an important role in researching the contribution of vascular endothelial cell during cerebral infarction development, preventing and curing by traditional Chinese medicine.
Chen
Yi
Y
Department of Physiology, Hunan University of Traditional Chinese Medicine, Changsha 410007, China.
Ge
Jin-wen
JW
Deng
Bing-xiang
BX
D000818:Animals; D002339:Carotid Arteries; D002544:Cerebral Infarction / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000473:pathology*; D007850:Lauric Acids / Q000008:administration & dosage; D008026:Ligation; D051381:Rats; D017207:Rats, Sprague-Dawley
D007850:Lauric Acids; C030358:lauric acid