[Expression of urotensin II and G-protein coupled receptor 14 mRNA in human pheochromocytoma tissues].
OBJECTIVE
To investigate the expression of urotensin II (U II) and G-protein coupled receptor 14 (GPR14) mRNA in human pheochromocytoma tissues.


METHODS
Total RNA from normal adrenal and pheochromocytoma tissues was extracted. The reverse transcription-polymerase chain reaction method was used to evaluate the levels of U II and GPR14 mRNA expression in human pheochromocytoma tissues.


RESULTS
There was no significant difference of U II and GPR14 mRNA expression between normal adrenal cortex and medulla. The expression of U II and GPR14 mRNA in pheochromocytoma was significantly lower than that in normal adrenal cortex and medulla (P < 0.05). The expression of GPR14 mRNA in adrenal pheochromocytomas was significantly lower than that of extra-adrenal pheochromocytomas (P < 0.05).


CONCLUSIONS
U II and GPR14 may play a role in the pathogenesis and hypertension regulating of pheochromocytoma.
Liu
Guo-Qiang
GQ
Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Zeng
Zheng-Pei
ZP
Li
Han-Zhong
HZ
Fan
Xin-Rong
XR
Liu
Dong-Mei
DM
Tong
An-Li
AL
Zheng
Xin
X
Liu
Chang
C
D000302:Adrenal Cortex / Q000378:metabolism; D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000313:Adrenal Medulla / Q000378:metabolism; D006801:Humans; D010673:Pheochromocytoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics; D014579:Urotensins / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C121697:UTS2R protein, human; D014579:Urotensins; C029677:urotensin II