Detection of phosphorus oxyanions in synthetic geothermal water using ion chromatography-mass spectrometry techniques.
Recent developments in microbiology suggest that reduced inorganic phosphorus oxyanions, including hypophosphite and phosphite, may be present in nature. These studies have inspired the development of specific and sensitive methods that detect phosphorus oxyanions in natural water. This paper will discuss a new technique that couples suppressed conductivity ion chromatography (Dionex AS17 analytical column and potassium hydroxide eluent) with electrospray mass spectrometry (IC/MS) with limits of detection nearly 200 times lower than those reported using suppressed conductivity detection. The technique was optimized for the detection of hypophosphite, phosphite, and phosphate in a synthetic geothermal water matrix. Samples were pre-treated with silver and sulfonic acid cartridges, and injection loop sizes as large as 800microl were employed to enhance instrument sensitivity. All peaks were clearly resolved, and calibrations were linear with estimated 3sigma limits of detection of 0.011, 0.0020, and 0.029microM for hypophosphite, phosphite, and phosphate, respectively.
Ivey
Michelle M
MM
Department of Chemistry and Biochemistry, California State University, 5151 State University Drive, Los Angeles, CA 90032-8282, USA.
Foster
Krishna L
KL
D002852:Chromatography, Ion Exchange / Q000379:methods*; D010710:Phosphates / Q000032:analysis*; D010721:Phosphinic Acids / Q000032:analysis* / Q000737:chemistry; D017905:Phosphites / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014867:Water / Q000737:chemistry
D010710:Phosphates; D010721:Phosphinic Acids; D017905:Phosphites; D014867:Water