Tularemia in Denmark: identification of a Francisella tularensis subsp. holarctica strain by real-time PCR and high-resolution typing by multiple-locus variable-number tandem repeat analysis.
We report ulceroglandular tularemia affecting an 8-year-old boy and the first recovery of Francisella tularensis in Denmark. A novel real-time PCR assay was used to identify the strain as F. tularensis subsp. holarctica (type B). Multiple-locus variable-number tandem repeat analysis demonstrated a close genetic relationship to strains from Norway.
Byström
Mona
M
Department of NBC-Analysis, Swedish Defence Research Agency, SE-901 82 Umeå, Sweden.
Böcher
Sidsel
S
Magnusson
Anna
A
Prag
Jørgen
J
Johansson
Anders
A
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002648:Child; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000032:analysis*; D003718:Denmark; D005604:Francisella tularensis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D014406:Tularemia / Q000175:diagnosis* / Q000382:microbiology
D004269:DNA, Bacterial