title: [Oxidative stress-induced accumulation of heat shock protein 70 within nucleolus].
abstract: OBJECTIVE
  To investigate the effect of oxidative stress on the accumulation of heat shock protein 70 (HSP70) within C2C12 myogenic cells.
  
  
  METHODS
  Heat shock response (42 degrees C for 1 h and recovery for 12 h at 37 degrees C) was used to induce the expression of heat shock protein 70. We constructed a recombinant plasmid of HSP70 with enhanced green fluorescent protein (EGFP). After being transfected transiently into C2C12 cells, immunoblotting was used to detect the expression of HSP70 induced by heat shock response and transfection. Immunocytochemistry, fluorescent microscopy and immunoblotting were used to detect the translocation of HSP70.
  
  
  RESULTS
  Immunoblotting showed that the overexpression of HSP70 was induced by heat shock response and transient transfenction. HSP70 localized within the cytoplasm of the normal cells, but HSP70 translocated from the cytoplasm to the nucleus and the nucleolus at 1 h after the treatment of oxidative stress (0.5 mmol/L H2O2) by using immunocytochemistry, fluorescent microscopy and immunoblotting for cellular partial proteins.
  
  
  CONCLUSIONS
  Oxidative stress may induce the accumulation of heat shock protein 70 within the nucleolus.
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Zi-zhi
authors.initials: ZZ
authors.lastname: Tu
authors.forename: Kang-kai
authors.initials: KK
authors.lastname: Wang
authors.forename: Jiang
authors.initials: J
authors.lastname: Zou
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Gong-hua
authors.initials: GH
authors.lastname: Deng
authors.forename: Xian-zhong
authors.initials: XZ
authors.lastname: Xiao
mesh_terms: D002466:Cell Nucleolus / Q000378:metabolism*; D002478:Cells, Cultured; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*
chemical_list: D018840:HSP70 Heat-Shock Proteins