Effects of geldanamycin on hatching and juvenile motility in Caenorhabditis elegans and Heterodera glycines.
Several Streptomyces species are known to produce metabolites that inhibit plant pathogens. One such compound is geldanamycin (GA), a benzoquinone ansamycin originally isolated from Streptomyces hygroscopicus. We examined the effect of geldanamycin on egg hatch and juvenile motility in Caenorhabditis elegans and in two populations of the plant-parasitic nematode Heterodera glycines. When C. elegans eggs were exposed to geldanamycin, both hatch and motility were reduced by GA doses between 2 and 50 microg/ml. The H. glycines inbred populations TN17 and TN18 exhibited low dose stimulation of hatch and motility, whereas levels occurring at higher GA doses were at or below control levels. These experiments represent the first demonstration of geldanamycin effects in C. elegans and H. glycines and suggest that the heat shock chaperone Hsp90, the known molecular target of geldanamycin, may be involved in nematode egg hatch and motility. This study also indicates that geldanamycin-producing strains of Streptomyces may be useful as biocontrol agents for nematodes.
Skantar
Andrea M
AM
Nematology Laboratory, USDA-ARS, Beltsville, MD, 20705, USA. skantara@ba.ars.usda.gov
Agama
Keli
K
Meyer
Susan L F
SL
Carta
Lynn K
LK
Vinyard
Bryan T
BT
D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology; D016227:Benzoquinones; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism / Q000494:pharmacology; D047029:Lactams, Macrocyclic; D007814:Larva / Q000187:drug effects* / Q000254:growth & development; D008297:Male; D011809:Quinones / Q000494:pharmacology*; D014431:Tylenchoidea / Q000187:drug effects* / Q000502:physiology
D000969:Antinematodal Agents; D016227:Benzoquinones; D004791:Enzyme Inhibitors; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D011809:Quinones; C001277:geldanamycin