title: Simple colorimetric method for detecting degenerate strains of the cultivated basidiomycete Flammulina velutipes (Enokitake).
abstract: Degeneration of cultivated strains of Flammulina velutipes is a serious problem. We developed a simple colorimetric method to detect degenerate strains by using a liquid medium supplemented with bromothymol blue and lactose. The ability of a strain to develop normal mushrooms could be determined by the color of the medium.
authors.affiliation: Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan. ymagae@ffpri.affrc.go.jp
authors.forename: Yumi
authors.initials: Y
authors.lastname: Magae
authors.forename: Kobun
authors.initials: K
authors.lastname: Akahane
authors.forename: Kimiyoshi
authors.initials: K
authors.lastname: Nakamura
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Tsunoda
mesh_terms: D000363:Agaricales / Q000254:growth & development* / Q000302:isolation & purification; D001979:Bromthymol Blue / Q000378:metabolism*; D003124:Colorimetry / Q000379:methods*; D003470:Culture Media; D048690:Fruiting Bodies, Fungal / Q000254:growth & development*; D007218:Industrial Microbiology / Q000379:methods*; D007564:Japan; D007785:Lactose / Q000378:metabolism
chemical_list: D003470:Culture Media; D007785:Lactose; D001979:Bromthymol Blue