title: Influence of dietary long-chain n-3 fatty acids from menhaden fish oil on plasma concentrations of alpha-tocopherol in geriatric beagles.
abstract: OBJECTIVE
  To determine effects of dietary n-3 fatty acids from Menhaden fish oil on plasma alpha-tocopherol concentrations in Beagles.
  
  
  METHODS
  32 female Beagles.
  
  
  METHODS
  For 82 days, dogs were fed diets that contained 1 of 2 ratios of n-6:n-3 fatty acids (40:1 [low n-3] and 1.4:1 [high n-3]) and 1 of 3 concentrations of all-rac-alpha-tocopheryl acetate (low, 17 mg/kg of diet; medium, 101 mg/kg; and high, 447 mg/kg) in a 2 X 3 factorial study.
  
  
  RESULTS
  Diets high in n-3 fatty acids significantly increased total content of n-3 fatty acids in plasma (17.0 g/100 g of fatty acids), compared with low n-3 diets (2.02 g/100 g of fatty acids). Mean +/- SEM plasma concentration of cholesterol was significantly lower in dogs consuming high n-3 diets (4.59 +/- 0.48 mmol/L), compared with dogs consuming low n-3 diets (5.71 +/- 0.48 mmol/L). A significant interaction existed between the ratio for n-6 and n-3 fatty acids and amount of alpha-tocopheryl acetate in the diet (plasma alpha-tocopherol concentration expressed on a molar basis), because the plasma concentration of alpha-toco-pherol was higher in dogs consuming low n-3 diets, compared with those consuming high n-3 diets, at the 2 higher amounts of dietary alpha-tocopheryl acetate. Plasma alpha-tocopherol concentration expressed relative to total lipid content did not reveal effects of dietary n-3 fatty acids on concentration of alpha-tocopherol.
  
  
  CONCLUSIONS
  Plasma alpha-tocopherol concentration is not dependent on dietary ratio of n-6 and n-3 fatty acids when alpha-tocopherol concentration is expressed relative to the total lipid content of plasma.
authors.affiliation: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331-4802, USA.
authors.forename: Jean A
authors.initials: JA
authors.lastname: Hall
authors.forename: Katie A
authors.initials: KA
authors.lastname: Tooley
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Gradin
authors.forename: Dennis E
authors.initials: DE
authors.lastname: Jewell
authors.forename: Rosemary C
authors.initials: RC
authors.lastname: Wander
mesh_terms: D000375:Aging / Q000097:blood*; D000821:Animal Feed; D000818:Animals; D004032:Diet / Q000662:veterinary*; D004285:Dogs / Q000097:blood*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000032:analysis; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage* / Q000737:chemistry; D024502:alpha-Tocopherol / Q000097:blood*
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; C033266:Menhaden oil; D024502:alpha-Tocopherol