title: Hen egg white fractionation by ion-exchange chromatography.
abstract: Major hen egg white proteins have been widely studied for their functional properties but these studies still are unable to explain, alone, all of the biological properties of hen egg white. Hence, it is still interesting to produce pure and non-altered proteins to improve our knowledge on the biological properties of hen egg white. Presently, identification and characterization of both bioactive peptides and minor proteins from hen egg white is essential work for progressing in the understanding of hen egg white biological properties. With this objective in mind, a new process for a complete "mucin free" hen egg white fractionation based on ion exchange chromatography is proposed. "Mucin free" egg white is fractionated into six different fractions. Four of them are high-recovery yield purified fractions of lysozyme, ovotransferrin, ovalbumin and flavoprotein. The two other fractions are enriched in recently detected minor proteins in hen egg white.
authors.affiliation: UMR 1253 Agrocampus Rennes-INRA, Sciences et technologie du lait et de l'oeuf, 65 rue de Saint-Brieuc, CS 84215, 35042 Rennes, France. Catherine.guerin@agrocampus-rennes.fr
authors.forename: C
authors.initials: C
authors.lastname: Guérin-Dubiard
authors.forename: M
authors.initials: M
authors.lastname: Pasco
authors.forename: A
authors.initials: A
authors.lastname: Hietanen
authors.forename: A
authors.initials: A
authors.lastname: Quiros del Bosque
authors.forename: F
authors.initials: F
authors.lastname: Nau
authors.forename: T
authors.initials: T
authors.lastname: Croguennec
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003207:Conalbumin / Q000302:isolation & purification; D004529:Egg White / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005420:Flavoproteins / Q000302:isolation & purification; D007525:Isoelectric Focusing; D013058:Mass Spectrometry; D009113:Muramidase / Q000302:isolation & purification; D010047:Ovalbumin / Q000302:isolation & purification
chemical_list: D005420:Flavoproteins; D003207:Conalbumin; D010047:Ovalbumin; D009113:Muramidase