Plasmid-encoded GM-CSF induces priming of the F4(K88)-specific serum IgA response by FaeG DNA vaccination in pigs.
We have used FaeG DNA to immunise piglets by the intradermal (ID) and the intramuscular (IM) route in a heterologous prime/boost model. ID immunisation with DNA resulted in a better induction of cellular immunity, whereas only the IM immunisation could prime an F4(K88)-specific serum IgA response. However, ID administration of plasmid-encoded GM-CSF 1 week before the ID immunisation enhanced the F4-specific humoral and cellular immune response and even primed the F4-specific IgA response more efficiently than the IM immunisation did.
Melkebeek
V
V
Laboratory of Veterinary Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. Vesna.Melkebeek@ugent.be
Verdonck
F
F
Stuyven
E
E
Goddeeris
B
B
Cox
E
E
D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000818:Animals; D049109:Cell Proliferation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000494:pharmacology*; D007070:Immunoglobulin A / Q000097:blood*; D008214:Lymphocytes / Q000166:cytology; D010957:Plasmids; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology
D018830:Adhesins, Escherichia coli; C494388:FaeG protein, E coli; D007070:Immunoglobulin A; D019444:Vaccines, DNA; D016178:Granulocyte-Macrophage Colony-Stimulating Factor