A common CYP1B1 polymorphism is associated with 2-OHE1/16-OHE1 urinary estrone ratio.
Cytochrome P450 (CYP) is a multigene family of enzymes involved in important life functions; some of these genes are inducible and are implicated in the oxidative metabolic activation and detoxification of many endogenous and exogenous compounds. CYP1B1 codes for an enzyme that catalyses the production of a 2- and 4-hydroxyl group in estrone and estradiol, while CYP1A1 catalyzes the 2-hydroxylation of estradiol in endometrium. The two genes were evaluated in a cohort of 150 subjects: African-American women had significantly lower 2-hydroxyl estrone/16-hydroxyl estrone (2-OHE1/16-OHE1) urinary metabolite ratios than Caucasian women (2.06+/-1.05 vs. 1.43+/-0.56; p=0.0002). A common polymorphism in the CYP1B1 gene (leucine to valineat codon 432) was associated with changes in urinary estrogen levels: both Caucasian and African-American women carrying the variant allele showed higher urinary metabolite ratios than women with the wild-type allele. No effect of the CYP1A1 MspI was observed. The 4-OHE1/2-OHE1 ratio was lower in subjects carrying the variant allele (Leu). The percentage change in 2-OHE1/16-OHE1 urinary ratio after indole treatment was significant in both Caucasian and African-American women carrying the wild-type CYP1B1 genotype, although it was more evident in African-Americans than in Caucasians. These results suggest that the Leu/Val CYP1B1 polymorphism may modify estradiol metabolism.
Paracchini
Valentina
V
Molecular and Genetic Epidemiology Unit, Fondazione Ospedale Policlinico IRCCS, Milan, Italy.
Pedotti
Paola
P
Raimondi
Sara
S
Garte
Seymour
S
Bradlow
H Leon
HL
Sepkovic
Daniel W
DW
Taioli
Emanuela
E
D000328:Adult; D001741:Black or African American / Q000235:genetics; D019943:Amino Acid Substitution; D001189:Aryl Hydrocarbon Hydroxylases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D065633:Cytochrome P-450 CYP1B1; D004247:DNA / Q000235:genetics; D019003:Deoxyribonuclease HpaII; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005828:Genetics, Population; D006801:Humans; D006894:Hydroxyestrones / Q000652:urine*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D044465:White People / Q000235:genetics
D006894:Hydroxyestrones; C026702:16-hydroxyestrone; D004958:Estradiol; D004247:DNA; D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1; D019003:Deoxyribonuclease HpaII; C024980:2-hydroxyestrone