Investigation on the origin of 5-HMF in Shengmaiyin decoction by RP-HPLC method.
The origin of 5-HMF (5-hydroxymethyl-2-furaldehyde) in a Shengmaiyin decoction was investigated by the RP-HPLC method below. A C(18) column (250 mm x 4.6 mm, i.d. 5 microm) with a column temperature of 25 degrees C was used. The mobile phase was a mixture of ultra-pure water-acetonitrile (95:5, V/V) and the flow rate was 1.0 ml/min. The detection wavelength was 280 nm. The injection volume was 1 microl and the running time was about 20 min. The addition of Schisandra was regulated to assess the contribution of an acid environment to the production of 5-HMF. In order to confirm the role of saccharides in the production of 5-HMF, different amount of fructose was used. The 5-HMF level in decoctions of processed and unprocessed Schisandra was investigated in order to determine the origin of 5-HMF. The results showed that 5-HMF was derived mainly from the decoction of Schisandra only and not the mixed decoction of Ophionpogon and Schisandra. The appearance of 5-HMF is not simply the result of the decomposition of saccharides under the acid environment created by Schisandra, but the processing procedure plays an important role in the production of 5-HMF.
Li
Ying-Hua
YH
Institute of Pharmaceutical Engineering, Zhejiang University, Hangzhou 310027, China.
Lu
Xiu-Yang
XY
D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005632:Fructose / Q000378:metabolism; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D032141:Schisandra / Q000737:chemistry
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C009252:fructus schizandrae, radix ginseng, radix ophiopogonis drug combination; D005632:Fructose; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde