title: Increasing of HER2 membranar density in human glioblastoma U251MG cell line established in a new nude mice model.
abstract: Glioblastoma multiform (GBM) remains the most devastating primary tumour in neuro-oncology. Human Epithelial Receptor Type 2 (HER2) is a transmembrane tyrosine/kinase receptor that is important for cancer growth. HER2 is not expressed in adult glial cells, but its expression increases with the degree of astrocytomas anaplasia. We have recently demonstrated the ability of anti-HER2 antibodies to induce in vitro apoptosis GBM cell lines; this ability is correlated to HER2 density. A decreasing of tyrosine/kinase receptors density during in vitro culture was reported. No information exists about the variation of HER2 expression after in vivo implantation. For that, the two cell lines in vitro tested (U251MG, A172) were in vivo implanted. We established a U251MG in vivo model in balb/c nude mice showing an important increasing of HER2 density. The HER2 density is correlated to anti-HER2 antibody efficiency so this model will be useful for the evaluation of in vivo anti-HER2 antibody treatment.
authors.affiliation: Department of Neurosurgery, Hospital Roger Salengro, University Hospital of Lille, 59037, Lille, France. j-mineo@chru-lille.fr
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Mineo
authors.forename: Anne
authors.initials: A
authors.lastname: Bordron
authors.forename: Isabelle
authors.initials: I
authors.lastname: Quintin-Roué
authors.forename: Quintin-Roué
authors.initials: QR
authors.lastname: Isabelle
authors.forename: Claude-Alain
authors.initials: CA
authors.lastname: Maurage
authors.forename: Virginie
authors.initials: V
authors.lastname: Buhé
authors.forename: Buhé
authors.initials: B
authors.lastname: Virginie
authors.forename: Séverine
authors.initials: S
authors.lastname: Loisel
authors.forename: Loisel
authors.initials: L
authors.lastname: Séverine
authors.forename: François
authors.initials: F
authors.lastname: Dubois
authors.forename: Serge
authors.initials: S
authors.lastname: Blond
authors.forename: Christian
authors.initials: C
authors.lastname: Berthou
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D017690:Cell Transplantation; D004195:Disease Models, Animal; D005434:Flow Cytometry; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D020893:Receptor, ErbB-3 / Q000378:metabolism*
chemical_list: D020893:Receptor, ErbB-3