Detecting the polymorphisms of paraoxonase (PON) cluster in Chinese Han population based on a rapid method.
BACKGROUND
An increased risk of coronary heart disease has been shown to be associated with polymorphisms in PON1 gene in different populations. Polymorphisms in PON2 gene have been associated with the level of plasma lipoproteins and glucose and are thought to play a role in atherosclerosis.


METHODS
To detect PONs polymorphisms more rapidly and reliably, we modified and improved the method established by Motti et al. We redesigned the primer for amplifying the common polymorphism at position 311 of PON2, which produced more reliable and efficient amplification.


RESULTS
A second common polymorphism at codon 148 was also detected by our new method, as were the 2 polymorphisms in the PON1 gene. The new method allowed identification of 4 polymorphisms (PON1-192, PON1-55, PON2-148 and PON2-311) simultaneously. The PONs genotypes of 82 healthy persons were identified by this method. The allelic frequencies were: PON1-192: Q 46.3%, R 53.7%; PON1-55: L 95.1%, M 4.9%; PON2-148: A 85.4%, G 14.6%; PON2-311: S 77.4%, and C 22.6%, respectively.


CONCLUSIONS
This method represents a simple, economical and time-saving technique to simultaneously detect 4 polymorphisms in the PON cluster. It provides a useful application to enable further study of the relationship between PON1 and PON2 and their role in atherosclerosis.
Zhang
Ying
Y
Center for Gene Diagnosis, Zhongnan Hospital, Wuhan University, Wuhan 430071, P.R. China.
Zheng
Fang
F
Du
Hansong
H
Krepinsky
Joan C
JC
Segbo
Julien A G
JA
Zhou
Xin
X
D000368:Aged; D043303:Aryldialkylphosphatase / Q000235:genetics*; D001483:Base Sequence; D002681:China; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D017931:DNA Primers; D043303:Aryldialkylphosphatase