Albumin attenuates neutrophil activation induced by stimulators including antibodies against neutrophil-specific antigens.
As neutrophil activation is related to several adverse transfusion reactions, we studied about the activation induced by anti-neutrophil antibodies and the stabilizing effects of albumin pretreatment by means of flow cytometry. Anti-neutrophil monoclonal antibody (anti-HNA-1a, 1b, 2a) alone induced CD11b expression and shedding of L-selectin, and anti-HNA-1a reinforced reactive oxygen species (ROS) production. Albumin pretreatment significantly reduced CD11b expression and L-selectin shedding induced by fMLP and ROS production induced by PMA, G-CSF combined with PMA or LPS-fMLP, or anti-HNA-1a combined with PMA. These findings suggest that anti-HNA-1a is related to adverse reactions and albumin has a regulating effect on neutrophil activation.
Hashimoto
Makoto
M
Blood Transfusion Division, Kobe University Hospital, Chuo-ku, Kobe 650-0017, Japan.
Saigo
Katsuyasu
K
Jyokei
Yukie
Y
Kishimoto
Manami
M
Takenokuchi
Mariko
M
Araki
Nobuo
N
Imoto
Shion
S
Taniguchi
Kikuyo
K
Kumagai
Shunichi
S
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D039481:CD11b Antigen / Q000096:biosynthesis*; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D019041:L-Selectin / Q000276:immunology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000276:immunology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects* / Q000276:immunology; D009504:Neutrophils / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D039481:CD11b Antigen; D002273:Carcinogens; D008562:Membrane Glycoproteins; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D019041:L-Selectin; D016179:Granulocyte Colony-Stimulating Factor; D009240:N-Formylmethionine Leucyl-Phenylalanine; D013755:Tetradecanoylphorbol Acetate