Follicle-stimulating hormone receptor gene mutations are not evident in Greek women with premature ovarian failure and poor responders.
OBJECTIVE
This clinical and molecular study aimed to investigate the presence of follicle-stimulating hormone (FSH) receptor gene mutations in women with premature ovarian failure (POF) and poor responders to in vitro fertilization treatment.


METHODS
DNA was extracted from blood samples for subsequent polymerase chain reaction (PCR). PCR was followed by restriction fragment length polymorphism and direct sequencing.


RESULTS
No inactivating mutations reported so far were identified in exons 6, 7, and 10 in women with POF and poor responders.


CONCLUSIONS
FSH receptor gene mutations are not frequent in Greek patients with POF as is the case in the rest of the world except for cases with ovarian dysgenesis in Finland.
Loutradis
Dimitris
D
Division of Human Reproduction, IVF Unit, 1st Department of Obstetrics and Gynecology, Athens University Medical School, Greece.
Patsoula
Eleni
E
Stefanidis
Konstantinos
K
Drakakis
Peter
P
Antonakis
Georgios
G
Bletsa
Ritsa
R
Antsaklis
Aris
A
Michalas
Stylianos
S
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D004247:DNA / Q000097:blood / Q000737:chemistry; D005091:Exons; D005260:Female; D006115:Greece / Q000453:epidemiology; D006801:Humans; D009154:Mutation; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016649:Primary Ovarian Insufficiency / Q000453:epidemiology / Q000235:genetics*; D011962:Receptors, FSH / Q000235:genetics*
D011962:Receptors, FSH; D004247:DNA