title: Identification of a novel substrate for tyrosine kinase in human testes.
abstract: Identification of genes specifically expressed in adult and fetal testes is important in furthering our understanding of testis development and function. In this study, a novel human specific transcript, designated NYD-SP10, was identified by hybridization of adult and fetal human testis probes with a human cDNA microarray. NYD-SP10 (GenBank Accession no. AF332192) was expressed at a higher level in adult testes than in fetal testes. Bioinformatic analysis illustrated NYD-SP10 is a splice variant of regulatory factor X4, while it is lack of RFX DNA-binding domain which binds to the X box of MHC class II genes. Most telling of all, NYD-SP10 uniquely possesses a tyrosine kinase phosphorylation site. Expression profiles demonstrated NYD-SP10 is specifically and strongly expressed in human adult testes and ejaculated spermatozoa. Taken together, these results suggest that NYD-SP10 is a novel testis-specific gene and it is possibly a novel substrate for tyrosine kinase and involved in the function of human spermatozoa, especially during capacitation.
authors.affiliation: Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029, P R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Huang
authors.forename: Zuomin
authors.initials: Z
authors.lastname: Zhou
authors.forename: Lanlan
authors.initials: L
authors.lastname: Yin
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.forename: Lirong
authors.initials: L
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.forename: Yadong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jiahao
authors.initials: J
authors.lastname: Sha
mesh_terms: D000328:Adult; D017398:Alternative Splicing; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005333:Fetus / Q000201:enzymology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008969:Molecular Sequence Data; D036103:Nanotechnology; D020411:Oligonucleotide Array Sequence Analysis; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism*; D000071701:Regulatory Factor X Transcription Factors; D013094:Spermatozoa / Q000201:enzymology; D013379:Substrate Specificity; D013737:Testis / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases