Expression of heme oxygenase-1 in the peripheral blood mononuclear cells from asthmatic patients.
To explore the expression of heme oxygenase-1 (HO-1) in the peripheral blood mononuclear cells (PBMCs) and its relationship with pulmonary ventilation function in asthmatic patients, 18 asthmatic patients and 18 healthy subjects were selected. HO-1 protein and mRNA levels in PBMCs were measured by immunohistochemical staining and reverse transcription-polymerase chain reaction (RT-PCR), respectively. Blood carbon monoxide Hb (COHb), serum total IgE and pulmonary ventilatory function were observed. Our results showed that the percentage of cells positive for immunohistochemical staining of HO-1 were significantly higher in asthmatic patients (41.72 +/- 7.44) % than that in with healthy subjects (10.45 +/- 4.36) % (P < 0.001) and the optical density of PBMC HO-1 mRNA was higher in asthmatic patients (26.05 +/- 4.14) than that in healthy subjects (10. 82 +/- 4.26) (P < 0.001). The relation analysis showed that PBMC HO-1 protein and mRNA levels had significantly negative relation with FEV1%, PEFR, MEFR50%, respectively (r = -0.51-0.89, P < 0.05-0.001, respectively) and a positive relation with COHb and serum total IgE (r = 0.48-0. 85, 0.05-0.001, respectively). It is concluded that the expression of PBMC HO-1 protein and mRNA increased significantly in asthmatic patients, and HO-1 may play a significant role in the pathogenesis of asthma. The expression of HO-1 may bear a relation with severity of asthma.
Mo
Biwen
B
Department of Respiratory Medicine, Affiliated Hospital of Guilin Medical College, Guilin 541001, China.
Zhang
Zhenxiang
Z
Xu
Yongjian
Y
Xiong
Weining
W
Liu
Xiansheng A
XA
Zhen
Guohua
G
D000328:Adult; D001249:Asthma / Q000097:blood / Q000201:enzymology*; D002248:Carbon Monoxide / Q000097:blood; D005260:Female; D051547:Heme Oxygenase-1 / Q000096:biosynthesis* / Q000097:blood; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood
D012333:RNA, Messenger; D007073:Immunoglobulin E; D002248:Carbon Monoxide; D051547:Heme Oxygenase-1