Composition gradient static light scattering: a new technique for rapid detection and quantitative characterization of reversible macromolecular hetero-associations in solution.
A method for rapid detection and quantitative characterization of associations between two proteins in solution is presented, based upon continuous measurement of the Rayleigh light scattering and UV absorbance of a solution whose composition is varied with time in a controlled fashion. Collection and subsequent analysis of data are both rapid and semiautomatic. The results of validation experiments demonstrate that the technique can correctly identify complexes and reliably evaluate equilibrium constants for hetero-association.
Attri
Arun K
AK
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, MD 20892, USA.
Minton
Allen P
AP
D002918:Chymotrypsin / Q000737:chemistry; D005340:Fibrinogen / Q000737:chemistry; D008027:Light; D011506:Proteins / Q000737:chemistry*; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions; D014361:Trypsin Inhibitors / Q000737:chemistry
D011506:Proteins; D012996:Solutions; D014361:Trypsin Inhibitors; D012710:Serum Albumin, Bovine; D005340:Fibrinogen; D002918:Chymotrypsin