[The significance of IFN-gamma measurement in middle ear effusion of secretory otitis media].
OBJECTIVE
To explore the clinical significance of IFN-gamma in secretory otitis media (SOM) by measuring and analyzing and the level of INF-gamma in the effusion of middle ear.


METHODS
A commercially available enzyme-linked immunosorbent assay (ELISA) incorporating a biotinylated monoclonal antibody probe was utilized for the quantitative measurement of IFN-gamma in the middle ear effusion and serum samples of the patients and that of the normal persons.


RESULTS
The level of IFN-gamma in the middle ear effusion was much higher than that in the serum samples. The difference of the level of IFN-gamma in the serum samples is not significant between the test group and the control group. The level of IFN-gamma in chronic period was much higher that in acute period. The level of IFN-gamma in the middle ear effusion has no significant difference between the group undergoing tympanocentesis for the first time and the group undergoing tympanocentesis for the second time (P > 0.05). The levels of IFN-gamma in the middle ear effusion in the group undergoing tympanocentesis for three or more times arose obviously (P < 0.01).


CONCLUSIONS
The IFN-gamma in the middle ear effusion may be produced by local tympanum,but not exuded simply from blood. The high expression of IFN-gamma in the middle ear effusion may be a reference of SOM tending to chronic course.
Chen
Suijun
S
Department of Otorhinolaryngology, the Second Affiliated Hospital, Sun-Yat-Sen University, Guangzhou, 510120, China.
Liang
Xiangfeng
X
Zheng
Yiqing
Y
Liu
Wei
W
Liu
Xiang
X
Long
Hua
H
Zheng
Pengling
P
D000293:Adolescent; D000328:Adult; D012634:Bodily Secretions / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010034:Otitis Media with Effusion / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
D007371:Interferon-gamma