title: [Effects of Shenggu injection on mRNA expression of vascular endothelia growth factor in rat osteoblasts in vitro].
abstract: OBJECTIVE
  To study the effects of Shenggu injection (SGI) on mRNA expression of vascular endothelia growth factor (VEGF) in rat osteoblasts in vitro and to explore its possible molecular mechanisms in promoting fracture healing.
  
  
  METHODS
  Rat osteoblasts cultured in vitro were stimulated with SGI according to the protocol. The expression levels of VEGF mRNA in the cells in every group were examined by reverse-transcriptase ploymerase chain reaction (RT-PCR).
  
  
  RESULTS
  When osteoblasts were stimulated with different concentrations of SGI for 5 days, the expression of VEGF mRNA peaked with 1 mg/ml SGI on the 5th day. When treated with 1 mg/ml SGI from the 1st to the 5th day, the expression of VEGF mRNA increased gradually with the increase of culturing time.
  
  
  CONCLUSIONS
  SGI could promote significantly the expression of VEGF mRNA in rat osteoblasts in vitro. The levels of expression of VEGF mRNA changed along with different concentrations and stimulating time of SGI.
authors.affiliation: Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. chengjiecjyy@yahoo.com.cn
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.forename: Rui
authors.initials: R
authors.lastname: Peng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yu
authors.forename: Yong-qing
authors.initials: YQ
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zou
authors.forename: Ren-yun
authors.initials: RY
authors.lastname: Xia
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D017102:Fracture Healing / Q000187:drug effects; D066298:In Vitro Techniques; D008516:Medicine, Chinese Traditional; D010006:Osteoblasts / Q000187:drug effects*; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A