Global regulation of X chromosomal genes by the MSL complex in Drosophila melanogaster.
A long-standing model postulates that X-chromosome dosage compensation in Drosophila occurs by twofold up-regulation of the single male X, but previous data cannot exclude an alternative model, in which male autosomes are down-regulated to balance gene expression. To distinguish between the two models, we used RNA interference to deplete Male-Specific Lethal (MSL) complexes from male-like tissue culture cells. We found that expression of many genes from the X chromosome decreased, while expression from the autosomes was largely unchanged. We conclude that the primary role of the MSL complex is to up-regulate the male X chromosome.
Hamada
Fumika N
FN
Howard Hughes Medical Institute, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
Park
Peter J
PJ
Gordadze
Polina R
PR
Kuroda
Mitzi I
MI
D000818:Animals; D002460:Cell Line; D004303:Dosage Compensation, Genetic; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D008297:Male; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology*; D014960:X Chromosome / Q000235:genetics / Q000378:metabolism*
D029721:Drosophila Proteins; D046912:Multiprotein Complexes