title: Single multiplex PCR assay to identify simultaneously the six categories of diarrheagenic Escherichia coli associated with enteric infections.
abstract: We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx(1), stx(2), and eae), enteropathogenic (eae and bfp), enterotoxigenic (st II and lt), enteroinvasive (vir F and ipa H), entero-aggregative (aaf II), and diffuse adherent (daa E) Escherichia coli in stool samples.
authors.affiliation: Programa de Microbiología, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile. rvidal@med.uchile.cl
authors.forename: Maricel
authors.initials: M
authors.lastname: Vidal
authors.forename: Eileen
authors.initials: E
authors.lastname: Kruger
authors.forename: Claudia
authors.initials: C
authors.lastname: Durán
authors.forename: Rosanna
authors.initials: R
authors.lastname: Lagos
authors.forename: Myron
authors.initials: M
authors.lastname: Levine
authors.forename: Valeria
authors.initials: V
authors.lastname: Prado
authors.forename: Cecilia
authors.initials: C
authors.lastname: Toro
authors.forename: Roberto
authors.initials: R
authors.lastname: Vidal
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology*; D004768:Enterotoxins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins