title: Haemonchus contortus: molecular cloning, sequencing, and expression analysis of the gene coding for the small subunit of ribonucleotide reductase.
abstract: Gastro-intestinal (GI) parasites are of great agricultural importance, annually costing the livestock industry vast amounts in resources to control parasitism. One such GI parasite, Haemonchus contortus, is principally pathogenic to sheep; with the parasite's blood-feeding behaviour causing effects ranging from mild anaemia to mortality in young animals. Current means of control, which are dependent on repeated treatment with anthelmintic chemicals, have led to increasing drug resistance. Together with the growing concern over residual chemicals in the environment and food chain, this has led to attempts to better understand the biology of the parasite with the aim to develop alternate or supplementary means of control, including the development of molecular vaccines. As a first step towards the understanding of the synthesis of deoxyribonucleotides in H. contortus, and its potential application to therapeutic control of this economically important parasite, we report the cloning, sequencing, and mRNA expression analysis of the ribonucleotide reductase R2 gene.
authors.affiliation: Primary Industries Research Victoria, Department of Primary Industries, 475 Mickleham Road, Attwood, Vic. 3049, Australia.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Chen
authors.forename: Debbie
authors.initials: D
authors.lastname: Donald
authors.forename: Keith
authors.initials: K
authors.lastname: Savin
authors.forename: Paul J A
authors.initials: PJ
authors.lastname: Presidente
authors.forename: Dadna
authors.initials: D
authors.lastname: Hartman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000829:Animals, Domestic; D002455:Cell Division / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation; D006189:Haemonchus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D007814:Larva / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012264:Ribonucleotide Reductases / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods; D012756:Sheep
chemical_list: D018076:DNA, Complementary; D018509:DNA, Helminth; D012333:RNA, Messenger; D012264:Ribonucleotide Reductases