title: [Moderate limbal deficiency in patients with congenital aniridia treated with amniotic membrane transplantation].
abstract: OBJECTIVE
  To study the ocular surface evolution by impression cytology and limbal biopsy in patients with congenital aniridia treated with amniotic membrane transplantation (AMT).
  
  
  METHODS
  We studied 14 eyes of patients with congenital aniridia and moderate limbal deficiency that were treated with AMT. The morphology of the limbocorneal epithelium and stroma was examined by impression cytology and limbal biopsy. Impression cytology was performed prior to surgery and 3, 6, 9, 12 and 24 months after treatment. Limbal biopsy was performed before surgery and 9 months after AMT.
  
  
  RESULTS
  Visual acuity showed a mean improvement of 0.3 at 24 months of follow-up. Corneal reepithelialization was completed by 2 months in all patients, however, after 9 months some patients showed persistent epithelial defects and chronic ulceration. Corneal clarity and peripheral neovascularization was markedly improved after 2 months. Impression cytology showed an improvement in squamous metaplasia at 3 and 6 months, however after nine months, until as late as 24 months, a progressive worsening was seen in epithelial cell metaplasia then being similar in extent to that before surgery. Limbal biopsy nine months after surgery showed a nearly normal epithelium and limbocorneal stroma.
  
  
  CONCLUSIONS
  AMT is a very effective procedure, although its effect is transitory, for restoring the ocular surface integrity in patients with congenital aniridia and moderate limbal deficiency.
authors.affiliation: Hospital Cruz Roja, Madrid, Spain. docsantilopez@hotmail.com
authors.forename: J S
authors.initials: JS
authors.lastname: López-García
authors.forename: L
authors.initials: L
authors.lastname: Rivas
authors.forename: I
authors.initials: I
authors.lastname: García-Lozano
mesh_terms: D000328:Adult; D000650:Amnion / Q000637:transplantation*; D015783:Aniridia / Q000473:pathology / Q000601:surgery*; D001706:Biopsy; D003316:Corneal Diseases / Q000473:pathology; D003319:Corneal Stroma / Q000166:cytology; D003584:Cytological Techniques; D019573:Epithelium, Corneal / Q000166:cytology / Q000473:pathology; D005500:Follow-Up Studies; D006801:Humans; D016850:Limbus Corneae / Q000473:pathology; D008679:Metaplasia; D008875:Middle Aged; D013997:Time Factors; D016896:Treatment Outcome; D014792:Visual Acuity