title: [Experimental model of primary cultured metastatic melanoma by fine needle aspiration].
abstract: OBJECTIVE
  Establish an experimental model of metastatic melanoma primary culture cells from fine-needle aspiration biopsies (FNAB).
  
  
  METHODS
  Two metastatic melanoma primary culture cells from (FNAB) have been developed from patients who had been submitted to excision of metastatic lesions and they were identified by immunohistochemical analyses using S-100 and MB-45 melanoma markers.
  
  
  RESULTS
  The culture diagnostic was confirmed by immunohistochemical. The technique of FNAB offers the advantage of providing a sequential analysis of the same tumor nodules throughout treatment, and is a minimally invasive procedure with almost no associated morbidity.
  
  
  CONCLUSIONS
  The establishment of metastatic melanoma primary culture from FNAB samples showed a viable technique.
authors.affiliation: Laboratório de Cultura de Células, Universidade Federal de São Paulo, Escola Paulista de Medicina (UNIFESP/EPM), São Paulo. drandreafernandes@terra.com.br
authors.forename: Andrea Fernandes de
authors.initials: AF
authors.lastname: Oliveira
authors.forename: Alfredo
authors.initials: A
authors.lastname: Gragnani
authors.forename: Renato Santos
authors.initials: RS
authors.lastname: Oliveira Filho
authors.forename: Ivan Dunshee de Abranches Oliveira
authors.initials: ID
authors.lastname: Santos
authors.forename: Silvana Gaiba de
authors.initials: SG
authors.lastname: França
authors.forename: Mílvia Maria Simões e Silva
authors.initials: MM
authors.lastname: Enokihara
authors.forename: Lydia Masako
authors.initials: LM
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D001707:Biopsy, Needle / Q000379:methods; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008545:Melanoma / Q000473:pathology* / Q000556:secondary; D009418:S100 Proteins / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D009418:S100 Proteins