Analysis of antibody response in goats to caprine herpesvirus 1.
Serum samples of goats experiencing natural and experimental infections and/or reactivation of caprine herpesvirus 1 (CpHV.1) were analysed with neutralization and Western blotting (WB) tests. WB immunological patterns resulted differently and related to neutralizing titers. In serum samples having neutralizing titer 1:2-1:4, antibodies to two proteins of Mw of 150 and 34 kDa were present. Antibodies against several proteins, two of those being characterized by monoclonal antibodies as gB and gC, were visualized by WB in sera having titer > or = 1:8. The neutralizing antibody titers and the pattern of antibody reactivity were hypothesized to modulate the reactivation and re-excretion process of CpHV.1.
Tempesta
Maria
M
Department of Animal Health and Well-being, Faculty of Veterinary Medicine, University of Bari, Strada provinciale per Casamassima km. 3, 70010 Valenzano, Italy. m.tempesta@veterinaria.uniba.it
Greco
Grazia
G
Tarsitano
Elvira
E
Thiry
Julien
J
Camero
Michele
M
Decaro
Nicola
N
Martella
Vito
V
Thiry
Etienne
E
Buonavoglia
Canio
C
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity / Q000276:immunology; D015511:Goat Diseases / Q000276:immunology* / Q000821:virology; D006041:Goats; D006566:Herpesviridae Infections / Q000276:immunology* / Q000662:veterinary*; D009500:Neutralization Tests; D018140:Varicellovirus / Q000276:immunology*
D000914:Antibodies, Viral