title: Drosophila E2F1 has context-specific pro- and antiapoptotic properties during development.
abstract: E2F transcription factors are generally believed to be positive regulators of apoptosis. In this study, we show that dE2F1 and dDP are important for the normal pattern of DNA damage-induced apoptosis in Drosophila wing discs. Unexpectedly, the role that E2F plays varies depending on the position of the cells within the disc. In irradiated wild-type discs, intervein cells show a high level of DNA damage-induced apoptosis, while cells within the D/V boundary are protected. In irradiated discs lacking E2F regulation, intervein cells are largely protected, but apoptotic cells are found at the D/V boundary. The protective effect of E2F at the D/V boundary is due to a spatially restricted role in the repression of hid. These loss-of-function experiments demonstrate that E2F cannot be classified simply as a pro- or antiapoptotic factor. Instead, the overall role of E2F in the damage response varies greatly and depends on the cellular context.
authors.affiliation: Massachusetts General Hospital Cancer Research Center, Charlestown, Massachusetts 02129, USA.
authors.forename: Nam-Sung
authors.initials: NS
authors.lastname: Moon
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Frolov
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Kwon
authors.forename: Luisa
authors.initials: L
authors.lastname: Di Stefano
authors.forename: Dessislava K
authors.initials: DK
authors.lastname: Dimova
authors.forename: Erick J
authors.initials: EJ
authors.lastname: Morris
authors.forename: Barbie
authors.initials: B
authors.lastname: Taylor-Harding
authors.forename: Kristin
authors.initials: K
authors.lastname: White
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Dyson
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D004249:DNA Damage; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000196:embryology* / Q000378:metabolism; D004628:Embryonic Structures / Q000033:anatomy & histology / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D017403:In Situ Hybridization; D009154:Mutation; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference
chemical_list: D029721:Drosophila Proteins; C108880:HID protein, Drosophila; D009479:Neuropeptides