Evaluation on the applied value of the dot immunogold filtration assay (DIGFA) for rapid detection of anti-Schistosoma japonicum antibody.
The dot immunogold filtration assay (DIGFA) is a rapid technique for the detection of anti-Schistosoma japonicum antibody. Its sensitivity with regard to sera obtained from patients with acute or chronic schistosomiasis was shown to be 100 and 96.9%, respectively. The specificity when using sera of people living in an area non-endemic for schistosomiasis japonica was 100%. Cross-reaction rates for paragonimiasis and clonorchiasis patients were 14.3% and 0%, respectively. Parallel serum tests of 1091 residents from an area endemic for S. japonicum by means of DIGFA, enzyme-linked immunosorbent assay and indirect haemagglutination test resulted in positive rates of 9.3%, 11.5% and 11.0%, respectively. Thus, there was a high level of agreement between the sets of results (P>0.05). In conclusion, DIGFA holds considerable promise for rapid and accurate diagnosis of S. japonicum, as it does not require any specific instruments and can be applied with ease. DIGFA has therefore several advantages over conventional diagnostic approaches and is useful not only for screening and sero-epidemiological surveys in the field, but also in clinical settings.
Wen
Li-Yong
LY
Research Laboratory of Molecular Immunoparasitology, Nanjing Medical University, Nanjing 210029, China. wenliyong@hzcnc.com
Chen
Jun-Hu
JH
Ding
Jian-Zu
JZ
Zhang
Jian-Feng
JF
Lu
Shao-Hong
SH
Yu
Li-Ling
LL
Shen
Li-Ying
LY
Wu
Guan-Ling
GL
Zhou
Xiao-Nong
XN
Zheng
Jiang
J
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D017979:Gold Colloid; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D011817:Rabbits; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D000909:Antibodies, Helminth; D017979:Gold Colloid