[Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province].
OBJECTIVE
To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene.


METHODS
PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program.


RESULTS
Newly isolated Japanese encephalitis viruses belonged to genotype III, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA14-14-2, but had some difference in some domains.


CONCLUSIONS
Newly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype III. E protein of the isolates showed some differences as compared with vaccine strains.
Chen
Duan
D
State Key Laboratory for Molecular Virology, Fujian Center for Disease Control and Prevention, Fuzhou 350001, China.
Wang
Huan-yu
HY
Fu
Shi-hong
SH
Song
Hong
H
Deng
Juan
J
Yang
Yi-liang
YL
Liang
Guo-dong
GD
D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004672:Encephalitis, Japanese / Q000821:virology*; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000235:genetics
D018076:DNA, Complementary; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C074932:prM protein, Flavivirus