Variability of flow rate when collecting stimulated human parotid saliva.
The aim of this study was to estimate the accuracy and reproducibility of citric-acid-stimulated parotid saliva sampling. In healthy volunteers a strong correlation (r2 = 0.79) between flow rates from the left and right parotid gland was observed. In patients with Sjögren's syndrome this correlation (r2 = 0.90) was even stronger. The intraindividual variation in healthy volunteers was 23.3 +/- 5.9%. Increasing the number of collections did not reduce this variation significantly. In head and neck cancer patients, to estimate whether repeated measurements result in more reliable baseline values for use in clinical studies, repeated collections did not result in a significant reduction of intrapatient variation, similar to the results with the healthy volunteers. Thus, notwithstanding the good agreement between left and right flow rates, a high variation in parotid flow rates has to be considered when planning clinical trials evaluating the effects of treatment on salivary gland functioning.
Burlage
Fred R
FR
Department of Radiotherapy, University of Groningen and University Medical Center Groningen, Groningen, the Netherlands. F.R.Burlage@rt.umcg.nl
Pijpe
Justin
J
Coppes
Rob P
RP
Hemels
Martin E W
ME
Meertens
Harm
H
Canrinus
Alof
A
Vissink
Arjan
A
D000328:Adult; D019343:Citric Acid / Q000494:pharmacology; D005260:Female; D006258:Head and Neck Neoplasms / Q000503:physiopathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism*; D015203:Reproducibility of Results; D012463:Saliva / Q000187:drug effects / Q000378:metabolism*; D012636:Secretory Rate / Q000187:drug effects / Q000502:physiology*; D012859:Sjogren's Syndrome / Q000503:physiopathology; D013997:Time Factors
D019343:Citric Acid