title: Global regulation of X chromosomal genes by the MSL complex in Drosophila melanogaster.
abstract: A long-standing model postulates that X-chromosome dosage compensation in Drosophila occurs by twofold up-regulation of the single male X, but previous data cannot exclude an alternative model, in which male autosomes are down-regulated to balance gene expression. To distinguish between the two models, we used RNA interference to deplete Male-Specific Lethal (MSL) complexes from male-like tissue culture cells. We found that expression of many genes from the X chromosome decreased, while expression from the autosomes was largely unchanged. We conclude that the primary role of the MSL complex is to up-regulate the male X chromosome.
authors.affiliation: Howard Hughes Medical Institute, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
authors.forename: Fumika N
authors.initials: FN
authors.lastname: Hamada
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Park
authors.forename: Polina R
authors.initials: PR
authors.lastname: Gordadze
authors.forename: Mitzi I
authors.initials: MI
authors.lastname: Kuroda
mesh_terms: D000818:Animals; D002460:Cell Line; D004303:Dosage Compensation, Genetic; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D008297:Male; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology*; D014960:X Chromosome / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D046912:Multiprotein Complexes