Saitohin, which is nested in the tau locus and confers allele-specific susceptibility to several neurodegenerative diseases, interacts with peroxiredoxin 6.
Saitohin is a gene unique to humans and their closest relatives, the function of which is not yet known. Saitohin contains a single polymorphism (Q7R), and its Q and R alleles belong to the H1 and H2 tau haplotype, respectively. The Saitohin Q allele confers susceptibility to several neurodegenerative diseases. To get a handle on Saitohin function, we used it as a bait in a yeast two-hybrid screen. By this assay and subsequent co-immunoprecipitation and glutathione S-transferase pull-down assays, we discovered and confirmed that Saitohin interacts with peroxiredoxin 6, a unique member of that family that is bifunctional and the levels of which increase in Pick disease. The strength of the interaction appeared to be allele-specific, giving the first distinction between the two forms of Saitohin.
Gao
Lei
L
Shriver Center at University of Massachusetts Medical School, Waltham, Massachusetts 02452, USA.
Tse
Sze-Wah
SW
Conrad
Christopher
C
Andreadis
Athena
A
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D004247:DNA / Q000235:genetics; D006239:Haplotypes; D020271:Heredodegenerative Disorders, Nervous System / Q000209:etiology / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism*; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins; D020774:Pick Disease of the Brain / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D016875:tau Proteins / Q000235:genetics* / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; C458340:STH protein, human; D016875:tau Proteins; D004247:DNA; D010544:Peroxidases; C515977:PRDX6 protein, human; D054465:Peroxiredoxin VI; D054464:Peroxiredoxins