title: [The interaction of vitamin B6 with the human serum albumin].
abstract: The Human Serum Albumin (HSA) can emit fluorescence (lamda(em) = 350 nm) under irradiation of ultraviolet light (lamda(ex) = 296 nm). After the vitamin B6 (B6) was added into HSA solution the fluorescence of HSA was quenched partially. This quenching was static quenching owing to the fact that ro (fluorescence life time (life time of the molecule excitation state) of HSA without B6) equaled to tau(i) (fluorescence life time of HSA with B6). The formation constant K between HSA and B6 was observed from the experiment, and then the binding distance R0 was determined (R0 = 1.872 nm). The CD spectra of samples (HSA, [HSA] = 1 x 10(-5) mol x L(-1), HSA +B6, [B6] = 5 x 10(-8), 1.5 x 10(-7), 2.5 x 10(-7), 3.5x 10(-7), 4.5x 10(-7) mol x L(-1)) were measured, and all CD spectra were almost the same. From [theta] values, the contents (percent content) of four structure(alpha-Helix, beta-Pleated, Beta-corner, and Randon wind) of samples can be calculated, and we find that all samples contain almost the same structure contents.
authors.affiliation: School of physics, Peking University, Beijing 100871, China
authors.forename: Jian-lin
authors.initials: JL
authors.lastname: Wang
authors.forename: Lian-chun
authors.initials: LC
authors.lastname: Fu
authors.forename: Shi-wu
authors.initials: SW
authors.lastname: Zhou
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Chen
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Lu
authors.forename: Xue-min
authors.initials: XM
authors.lastname: Ye
authors.forename: Guang-zheng
authors.initials: GZ
authors.lastname: Meng
authors.forename: Zeng-fu
authors.initials: ZF
authors.lastname: Song
mesh_terms: D000465:Algorithms; D001665:Binding Sites; D002942:Circular Dichroism; D005453:Fluorescence; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D025101:Vitamin B 6 / Q000737:chemistry* / Q000378:metabolism
chemical_list: D012709:Serum Albumin; D025101:Vitamin B 6