title: Frequency and distribution of chromosome abnormalities in human oocytes.
abstract: It was previously shown that more than half of the human oocytes obtained from IVF patients of advanced reproductive age are aneuploid, due to meiosis I and meiosis II errors. The present paper further confirms that 61.8% of the oocytes tested by fluorescent probes specific for chromosomes 13, 16, 18, 21 and 22 are abnormal, representing predominantly chromatid errors, which are the major source of aneuploidy in the resulting embryos. Almost half of the oocytes with meiosis I errors (49.3%) are prone to sequential meiosis II errors, which may lead to aneuploidy rescue in 30.8% of the cases. Half of the detected aneuploidies (49.8%) are of complex nature with involvement of two or more chromosomes, or the same chromosome in both meiotic divisions. The aneuploidy rates for individual chromosomes are different, with a higher prevalence of chromosome 21 and 22 errors. The origin of aneuploidy for the individual chromosomes is also not random, with chromosome 16 and 22 errors originating more frequently in meiosis II, and chromosome 18, 13 and 21 errors in meiosis I. There is an age dependence not only for the overall frequency of aneuploidies, but also for each chromosome error, aneuploidies originating from meiosis I, meiosis II, and both meiosis I and meiosis II errors, as well as for different types of aneuploidies. The data further suggest the practical relevance of oocyte aneuploidy testing for detection and avoidance from transfer of the embryos deriving from aneuploid oocytes, which should contribute significantly to the pregnancy outcomes of IVF patients of advanced reproduction age.
authors.affiliation: Reproductive Genetics Institute, Chicago, IL 60614, USA. anverkuliev@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Kuliev
authors.forename: J
authors.initials: J
authors.lastname: Cieslak
authors.forename: Y
authors.initials: Y
authors.lastname: Verlinsky
mesh_terms: D000328:Adult; D000375:Aging; D002869:Chromosome Aberrations / Q000706:statistics & numerical data*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008423:Maternal Age; D008540:Meiosis; D008597:Menstrual Cycle; D009865:Oocytes / Q000166:cytology / Q000502:physiology*