The effect of L-NAME administrations after oral mucosal incision on wound NO level in rabbit.
The aim of the current study was to comparatively investigate the effect of inhibition of nitric oxide (NO) production by N-nitro-L-arginine methyl ester (L-NAME), an isoform non-specific inhibitor of nitric oxide synthase (NOS), after oral mucosal incision on wound tissue NO levels. A standard incision was applied to the oral mucosa of rabbits. After oral mucosal incision, rabbits were divided into five groups as follows: (1) Untreated incisional group (control); (2) Titanium (Ti) implanted group; (3) Ti + Polyethylene glycol (PEG) 4000 implanted group; (4) Ti + PEG 4000 + L-NAME (2 x 10(-4) M) implanted group and (5) i.p. L-NAME administrated group (10 mg/kg). At 5 days after oral incision operations, wound tissue strips and plasma were obtained from rabbits. Oral wound tissue and plasma nitric oxide, plasma thiobarbituric acid reactive substances (TBARS) and total sulfhydryl group (RSH) levels were investigated. Plasma TBARS and NOx levels decreased after i.p. L-NAME administration. Total RSH group levels were not changed in all groups (p>0.05). This means that L-NAME inhibits the deteriorating effects of free radicals without affecting healing. L-NAME in PEG and titanium also has no effect on tissue and plasma NOx levels. These findings indicate that NO generation will not be affected both Ti and local nitric oxide synthase (NOS) inhibitor.
Coşkun
Sule
S
Department of Biology, Science & Art Faculty, Gazi University, Beşevler Ankara 06500, Turkey. sule@gazi.edu.tr
Karataş
Fehmi
F
Acartürk
Füsun
F
Olmuş
Hülya
H
Selvi
Mahmut
M
Erbaş
Deniz
D
D000818:Animals; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D009061:Mouth Mucosa / Q000293:injuries* / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism*; D011817:Rabbits; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014025:Titanium / Q000378:metabolism / Q000494:pharmacology
D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D014025:Titanium; D019331:NG-Nitroarginine Methyl Ester