title: Lack of association of histamine-N-methyltransferase (HNMT) polymorphisms with asthma in the Indian population.
abstract: Histamine plays a major role in allergic disorders, including asthma. A major pathway of histamine biotransformation in the lungs is mediated by histamine N-methyltransferase (HNMT). We investigated the association of a functional SNP C314T; a SNP A929G, a (CA)n repeat in intron 5, and a novel (CA)n repeat (BV677277), 7.5 kb downstream of the HNMT gene with asthma and its associated traits such as total serum IgE levels in a case-control as well as in a family-based study design. In contrast to a previous study, no association was observed for the polymorphisms investigated with asthma (P>0.05). When haplotypes were constructed for these loci and compared, no significant difference was observed in the distribution between cases and controls. In the family-based design, no biased transmission was observed for any of the polymorphisms and haplotypes with asthma using the additive model of inheritance in family-based association test (FBAT). Thus, consistent with the case-control findings, the polymorphisms and haplotypes in the HNMT gene are not associated with asthma in the Indian population.
authors.affiliation: Molecular Immunogenetics Laboratory, Institute of Genomics and Integrative Biology, Mall Road, 110007, Delhi, India.
authors.forename: Shilpy
authors.initials: S
authors.lastname: Sharma
authors.forename: Deepti
authors.initials: D
authors.lastname: Mann
authors.forename: Tej Pratap
authors.initials: TP
authors.lastname: Singh
authors.forename: Balaram
authors.initials: B
authors.lastname: Ghosh
mesh_terms: D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006637:Histamine N-Methyltransferase / Q000235:genetics*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007194:India / Q000453:epidemiology; D008297:Male; D011110:Polymorphism, Genetic
chemical_list: D007073:Immunoglobulin E; D006637:Histamine N-Methyltransferase