The influence of product instability on slow-binding inhibition.
We present a kinetic study of an enzyme reaction that takes place with slow-binding inhibition where the immediate product undergoes a spontaneous or induced process of decomposition. A kinetic study of an enzyme process, in which a slow-binding inhibition process and a decomposition of the immediate product of the reaction take place simultaneously is performed. The corresponding explicit concentration-time equations were obtained. Using the analytical solutions obtained, which were tested numerically, we suggest a procedure that allows the discrimination between the particular cases considered and the evaluation of the principal kinetic parameters of the reaction.
Garrido-Del Solo
C
C
Departamento de Química-Física, Escuela Politécnica Superior, Universidad de Castilla-La Mancha Avda, España, s/n Campus Universitario, E-02071 Albacete, Spain.
Yago
J M
JM
García-Moreno
M
M
Havsteen
B H
BH
García-Cánovas
F
F
Varón
R
R
D004791:Enzyme Inhibitors; D004798:Enzymes / Q000493:pharmacokinetics*; D008432:Mathematical Computing; D008962:Models, Theoretical; D013379:Substrate Specificity
D004791:Enzyme Inhibitors; D004798:Enzymes