[Analysis of part of M gene and genotyping for M segment of hantavirus detected from HFRS patients' sera in Qingdao region during 2000 - 2003].
OBJECTIVE
To study the molecular epidemiological characteristics of hantavirus seen during 2000-2003 in Qingdao region of Shandong province.


METHODS
Sera were collected from 64 patients with hemorrhagic fever with renal syndrome (HFRS) and viral RNA was extracted from the sera. HTN and SEO universal primers were designed as outer primers and HTN and SEO specific primers as inner primers. G1 gene region of M segment from hantavirus was amplified by using RT-nest-PCR for sequencing. The data of nucleotide sequences were analyzed by DNA star software.


RESULTS
Six cases were positive by HTN specific primer of total cases (9%); 25 of 64 cases by SEO specific primer (39%); total positive rate was 48%. In general, SEO type was a prevalent type of hantavirus in Qingdao region. The variation of the nucleotide sequences among SEO viruses (nucleotide sequence divergence ranged from 0.3% approximately 8.9%) was lower than that among HTN type (nucleotide sequence divergence ranged from 2.6% approximately 11.2% ).


CONCLUSIONS
Majority of hantavirus found in Qingdao region belonged to SEO type and still a few strains belonged to HTN type. Most of the HTN viruses were detected in Jiaonan county.
Qiao
Gang
G
The people's Hospital of Jiaonan, Jiaonan, China.
Pang
Shu-shen
SS
Zhang
Hong-le
HL
Gong
Yan
Y
Cheng
Mao-ling
ML
Li
Zhen-hai
ZH
D002681:China; D005838:Genotype; D006476:Orthohantavirus / Q000235:genetics*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000097:blood / Q000821:virology*; D006801:Humans; D012367:RNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014763:Viral Matrix Proteins / Q000235:genetics*
D012367:RNA, Viral; D014763:Viral Matrix Proteins