[Interaction between balofloxacin and DNA and the influence of Mg2+ on the interaction].
OBJECTIVE
To study the binding mode of balofloxacin with DNA and evaluate the influence of Mg2+ on the binding between balofloxacin and DNA.


METHODS
Fluorescent spectroscopy was used to study the interaction of balofloxacin with DNA and to calculate the thermodynamic constants. UV-Vis spectra, DNA viscosity titration, competition experiment and the effect of dsDNA and ssDNA on the fluorescense intensity were used to identify the binding mode.


RESULTS
Balofloxacin interacted with CT-DNA with a quenching constant of (5.43 +/- 0.07) x 10(3) L x mol(-1). The interaction was exothermic with a Van't Hoff enthalply of - 8.03 kJ x mol(-1) x Mg2+ cation could enhance the quenching constant between balofloxacin and DNA.


CONCLUSIONS
Balofloxacin interacted with CT-DNA in the mode of groove binding and Mg2+ could mediate the binding of balofloxacin to DNA.
Zhong
Wen-Ying
WY
Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210038, China. wenyingzhongnj@163.com
Huang
Qi
Q
Wang
Dan-Dan
DD
Ni
Kun-Yi
KY
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D024841:Fluoroquinolones / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D024841:Fluoroquinolones; D004247:DNA; D008274:Magnesium; C077155:balofloxacin