Role of epiregulin in peptidoglycan-induced proinflammatory cytokine production by antigen presenting cells.
We have previously found that epiregulin, a member of epidermal growth factor superfamily, is involved in proinflammatory cytokine production in bone marrow-derived macrophages. In this report, to further assess the role of epiregulin in innate immunity, we measured IL-6 production levels upon lipopolysaccharide and peptidoglycan stimulation in antigen presenting cells including macrophages and dendritic cells. Our analyses using epiregulin-deficient mice with mixed and inbred genetic backgrounds revealed that epiregulin deficiency results in the reduction of IL-6 production levels in both cell types upon peptidoglycan stimulation, and that the extent of this reduction is more evident under the BALB/c background compared with the C57BL/6J background. These results indicated that epiregulin may have a critical role in the regulation of peptidoglycan-mediated proinflammatory cytokine production in antigen presenting cells and innate immunity.
Sugiyama
Shigeru
S
Department of Pathology, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjyuku-ku, Tokyo 162-8655, Japan.
Nakabayashi
Kazuhiko
K
Baba
Iwai
I
Sasazuki
Takehiko
T
Shirasawa
Senji
S
D000818:Animals; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D004815:Epidermal Growth Factor / Q000235:genetics / Q000502:physiology*; D066260:Epiregulin; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000819:agonists; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010457:Peptidoglycan / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000819:agonists; D051193:Toll-Like Receptors
D066260:Epiregulin; C587881:Ereg protein, mouse; D018836:Inflammation Mediators; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D010457:Peptidoglycan; D011956:Receptors, Cell Surface; D051193:Toll-Like Receptors; D004815:Epidermal Growth Factor