The isolated perfused kidney: an in vitro test system for evaluation of renal tissue damage induced by high-energy shockwaves sources.
Most of our knowledge of shockwave-induced renal damage is based on animal experiments and clinical observation. We developed a tissue model using isolated porcine kidneys perfused with Berliner Blau dye in physiologic saline using a Ureteromat Perez-Castro peristaltic pump connected to the renal artery. Reproducible results were obtained under a variety of experimental conditions. Further refinements of the model might consist of interposition of tissue layers in the shockwave path or simulation of ventilatory movements.
Bergsdorf
Th
T
Department of Urology, Städt. Krankenhaus München-Harlaching, Munich, Germany.
Thüroff
S
S
Chaussy
Ch
Ch
D000818:Animals; D001466:Barium Sulfate; D003287:Contrast Media; D004912:Erythrocytes; D005456:Fluorescent Dyes; D019220:High-Energy Shock Waves / Q000009:adverse effects*; D066298:In Vitro Techniques; D007668:Kidney / Q000098:blood supply / Q000528:radiation effects*; D023421:Models, Animal; D012039:Regional Blood Flow; D012077:Renal Artery; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000737:chemistry; D013552:Swine; D014160:Transducers, Pressure
D003287:Contrast Media; D005456:Fluorescent Dyes; D001466:Barium Sulfate; D012965:Sodium Chloride