Nucleolar localization of potato leafroll virus capsid proteins.
Potato leafroll virus (PLRV) encodes two capsid proteins, major protein (CP) and minor protein (P5), an extended version of the CP produced by occasional translational 'readthrough' of the CP gene. Immunogold electron microscopy showed that PLRV CP is located in the cytoplasm and also localized in the nucleus, preferentially targeting the nucleolus. The nucleolar localization of PLRV CP was also confirmed when it was expressed as a fusion with green fluorescent protein (GFP) via an Agrobacterium vector. Mutational analysis identified a particular sequence within PLRV CP involved in nucleolar targeting [the nucleolar localization signal (NoLS)]. Minor protein P5 also contains the same NoLS, and was targeted to the nucleolus when it was expressed as a fusion with GFP from Agrobacterium. However, P5-GFP lost its nucleolar localization in the presence of replicating PLRV.
Haupt
Sophie
S
University of Dundee, Dundee DD1 4NH, UK.|Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
Stroganova
Tanya
T
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
Ryabov
Eugene
E
University of Warwick - HRI, Wellesbourne, Warwick CV35 9EF, UK.
Kim
Sang Hyon
SH
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
Fraser
Gill
G
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
Duncan
George
G
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
Mayo
Mike A
MA
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
Barker
Hugh
H
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
Taliansky
Michael
M
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
D002213:Capsid / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D049452:Green Fluorescent Proteins; D017793:Luteovirus / Q000235:genetics / Q000378:metabolism*; D012231:Rhizobium / Q000821:virology; D011198:Solanum tuberosum / Q000821:virology*
D049452:Green Fluorescent Proteins