Cell surface membrane antigen phenotype of human gastrointestinal mast cells.
BACKGROUND
Mast cells (MC) are important effector cells of allergic and inflammatory reactions in diverse organs. These cells interact with a number of other immune cells and structural cells in the tissues as well as with proinflammatory mediators and cytokines. The various interactions are considered to be mediated through distinct cell surface membrane receptors on MC.


METHODS
In the present study, we have established the cell surface membrane phenotype of human gastrointestinal MC (HGMC) using a panel of monoclonal antibodies and indirect immunofluorescence staining techniques.


RESULTS
HGMC were found to react with antibodies against CD29, CD33, CD44, CD45, CD47, CD54, CD55, CD58, CD63, CD117, CD147, CD151, CD172a, and CD203c. By contrast, HGMC did not express detectable amounts of CD1, CD2, CD4, CD5, CD14, CD15, CD16, CD22, CD24, CD25, CD26, CD27, CD28, CD31, CD32, CD34, CD35, CD88, or CD116. The alpha-chain of the IL-3 receptor (CD123) was detectable neither in resting HGMC nor in HGMC exposed to stem cell factor and interleukin-4.


CONCLUSIONS
HGMC express a unique profile of surface antigens including the receptor for mast cell growth factor, adhesion-related molecules, and activation-linked membrane antigens.
Krauth
Maria-Theresa
MT
Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.
Majlesi
Yasamin
Y
Florian
Stefan
S
Bohm
Alexandra
A
Hauswirth
Alexander W
AW
Ghannadan
Minoo
M
Wimazal
Friedrich
F
Raderer
Markus
M
Wrba
Friedrich
F
Valent
Peter
P
D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D002448:Cell Adhesion / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D041981:Gastrointestinal Tract / Q000166:cytology* / Q000276:immunology; D006801:Humans; D008407:Mast Cells / Q000276:immunology*; D010641:Phenotype; D018121:Receptors, Cytokine / Q000032:analysis
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D018121:Receptors, Cytokine