[The protective effects of sini decoction on mitochondrial function in adriamycin-induced heart failure rats].
OBJECTIVE
The role of mitochondria in Adriamycin (ADR) -induced heart failure and the protective effects of Sini Decoction (SND) were investigated.


METHODS
SD rats were randomly divided into three groups, control group, heart failure group (model group) and SND group. ADR was injected in the rats of heart failure group and SND group by caudal vein. After injection, the rats in SND group were given SND (3.75/kg/d, p. o.). Three weeks later, cardiac function, mitochondrial swelling, content of malondialdehyde (MDA), activity of Mn SOD, Na+-K+ ATPase and Ca2+ ATPase were measured. The mRNA expression of Mn SOD was also detected by RT-PCR.


RESULTS
Compared with model group, the cardiac function, the activity and the mRNA expression of Mn SOD and the activity of Na+-K+ ATPase and Ca2+ ATPase were significantly elevated, while the degree of mitochondria swelling and the content of MDA were reduced in SND-treated rats.


CONCLUSIONS
The data suggests that oxidative stress is present in the mitochondria of myocardium in ADR-induced heart failure rats and it can be reduced by SND. The mechanism may be closely related to the protective effects on mitochondria.
Zhao
Mingqi
M
Department of Pathophysiology, ZhongShan Medical College, Sun yat-sen University, Guangzhou.
Wu
Weikang
W
Duan
Xinfen
X
Liu
Ying
Y
Zhao
Danyang
D
Liang
Tianwen
T
Luo
Hanchuan
H
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004317:Doxorubicin / Q000633:toxicity; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006333:Heart Failure / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D004317:Doxorubicin; D013482:Superoxide Dismutase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase