Lipopolysaccharide binding protein, cytokine production in whole blood, and lipoproteins in cystic fibrosis.
According to the endotoxin lipoprotein hypothesis, lipoproteins may down-regulate cytokine production by neutralizing lipopolysaccharide (LPS) binding protein (LBP) complexes. We investigated the correlation between lipoproteins, LBP, cytokine production, and clinical status in Delta F 508 (homozygous) individuals. Cystic fibrosis patients with mild disease were compared with those with more severe disease and age-matched controls. LBP, IL-8, and tumor necrosis factor-alpha, using a chemiluminescent immunometric assay, and fat intake, as well as serum triglycerides, cholesterol, very low density lipoprotein, LDL, and HDL were measured. In more severe disease there was a correlation between maximum expiratory flow at 25% of vital capacity and HDL. To adjust for the influence of colonization with Pseudomonas aeruginosa, those who were colonized with P. aeruginosa were analyzed separately. There was a significant correlation between LBP and forced expiratory volume in 1 s. Lipoproteins may have a modulating effect in more advanced disease and are not influenced by fat intake. LBP correlates those who were colonized with P. aeruginosa (Psa+) with clinical status as well as lung function and may be a critical molecule regulating LPS-induced inflammation.
Schmitt-Grohé
Sabina
S
Children's Hospital Medical Center, University of Bonn, 53113 Bonn, Germany. s.schmitt.grohe@uni-bonn.de
Hippe
Valerie
V
Igel
Michael
M
von Bergmann
Karl
K
Posselt
Heinz G
HG
Krahl
Andreas
A
Smaczny
Christina
C
Wagner
Thomas O F
TO
Nikolazik
Wilfried
W
Schubert
Ralf
R
Lentze
Michael J
MJ
Zielen
Stefan
S
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000293:Adolescent; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000097:blood; D002648:Child; D003550:Cystic Fibrosis / Q000097:blood* / Q000235:genetics / Q000503:physiopathology; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000097:blood; D009154:Mutation; D012129:Respiratory Function Tests; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D016207:Cytokines; D008074:Lipoproteins; D008562:Membrane Glycoproteins; D000099231:Lipopolysaccharide-Binding Protein