[The effect of Siwu Tang on EPO and G-CSF gene expression in bone marrow of irradiated blood deficiency mice].
OBJECTIVE
To observe the blood enriching function of Siwu Tang and its effect on Epo and G-CSF gene expressions in bone marrow of blood deficiency mice, and thus provide the basis for understanding the molecular mechanism of blood enriching function of Siwu Tang.


METHODS
The animal model of blood deficiency were established in the mice by using 3.5 Gy60Co gamma-ray. Peripheral blood cells were analyzed and CFU-GM, BFU-E, CFU-E and CFU-mix were counted in bone marrow colony cultured. Both Epo and G-CSF gene expressions in bone marrow were measured with RT-PCR.


RESULTS
Siwu Tang significantly increased the number of peripheral blood cells and the amount of CFU-GM, BFU-E, CFU-E and CFU-mix in bone marrow and enhanced Epo and G-CSF gene expression in bone marrow in the mice with blood deficiency.


CONCLUSIONS
The promotion of Epo and G-CSF gene expressions in bone marrow may be one of the mechanisms underlying the blood enriching function of Siwu Tang decoction.
Guo
Ping
P
Institute of Radiation Medicine, Academy of Military Medical Sciences, Bejing 100850, China.
Liang
Qian-De
QD
Hu
Jian-Jiang
JJ
Wang
Ji-Feng
JF
Wang
Sheng-Qi
SQ
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology; D002452:Cell Count; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D015672:Erythroid Precursor Cells / Q000166:cytology; D004921:Erythropoietin / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D007958:Leukocyte Count; D008516:Medicine, Chinese Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014916:Whole-Body Irradiation
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C403258:si-wu-tang; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor