Protein inhibition of surfactant during mechanical ventilation of isolated rat lungs.
This study tested the hypothesis that material leaking into the airspace from the vasculature during ventilation interferes with surfactant function and contributes to decreases in lung compliance. Rats were euthanized and the lungs were isolated either with or without flushing of the vasculature, followed by mechanical ventilation and analysis of lung compliance and lung lavage analysis. Flushed lungs had higher lung compliance compared to the non-flushed lungs. This was associated with lower protein concentrations and improved surfactant activity. It is concluded that during mechanical ventilation, leakage of proteins results in surfactant inhibition and thereby contribute to decreased lung compliance.
Da Silva
Keith
K
Lawson Health Research Institute and Department of Physiology, The University of Western Ontario, London, Ontario, Canada.
McCaig
Lynda A
LA
Veldhuizen
Ruud A W
RA
Possmayer
Fred
F
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D008168:Lung / Q000098:blood supply / Q000378:metabolism / Q000502:physiology; D008170:Lung Compliance / Q000502:physiology; D008297:Male; D008954:Models, Biological; D011652:Pulmonary Circulation; D011663:Pulmonary Surfactants / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012121:Respiration, Artificial / Q000009:adverse effects*; D007507:Therapeutic Irrigation
D001798:Blood Proteins; D011663:Pulmonary Surfactants