Efficient strategy for the rapid backbone assignment of membrane proteins.
Investigations of membrane proteins pose one of the biggest current challenges in structural biology. Recent advances in protein production techniques based on cell-free transcription/translation methods have, however, opened new opportunities in this area. Here, we report an efficient protocol for the backbone assignment of membrane proteins as the first step of NMR-based structure determination.
Trbovic
Nikola
N
Institute for Biophysical Chemistry, University of Frankfurt and Center for Biomolecular Magnetic Resonance, 60439 Frankfurt, Germany.
Klammt
Christian
C
Koglin
Alexander
A
Löhr
Frank
F
Bernhard
Frank
F
Dötsch
Volker
V
D018360:Crystallography, X-Ray; D008565:Membrane Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation
D008565:Membrane Proteins