Simultaneous determination of rifampicin and isoniazid by continuous-flow chemiluminescence with artificial neural network calibration.
In this paper a continuous-flow chemiluminescence (CL) system with artificial neural network calibration is proposed for simultaneous determination of rifampicin and isoniazid. This method is based on the different kinetic spectra of the analytes in their CL reaction with alkaline N-bromosuccinimide as oxidant. The CL intensity was measured and recorded every second from 1 to 300 s. The data obtained were processed chemometrically by use of an artificial neural network. The experimental calibration set was 20 sample solutions. The relative standard errors of prediction for both analytes were approximately 5%. The proposed method was successfully applied to the simultaneous determination of rifampicin and isoniazid in a combined pharmaceutical formulation.
Li
Baoxin
B
Department of Chemistry, School of Chemistry and Materials, Shaanxi Normal University, Xi'an, 710062, PR China. libx29@hotmail.com
He
Yuezhen
Y
Lv
Jiagen
J
Zhang
Zhujun
Z
D000465:Algorithms; D000995:Antitubercular Agents / Q000032:analysis / Q000737:chemistry; D002138:Calibration; D045424:Complex Mixtures / Q000032:analysis / Q000737:chemistry; D004338:Drug Combinations; D017022:Flow Injection Analysis / Q000379:methods* / Q000592:standards; D007538:Isoniazid / Q000032:analysis* / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods* / Q000592:standards; D016571:Neural Networks, Computer; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012293:Rifampin / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity
D000995:Antitubercular Agents; D045424:Complex Mixtures; D004338:Drug Combinations; D007538:Isoniazid; D012293:Rifampin