Mass spectrometry in diagnostic oncoproteomics.
Diagnostic oncoproteomics is the application of proteomic techniques for the diagnosis of malignancies. A new mass spectrometric technology involves surface enhanced laser desorption ionization combined with time-of flight mass analysis (SELDI-TOF-MS), using special protein chips. After the description of the relevant principles of the technique, including approaches to proteomic pattern diagnostics, applications are reviewed for the diagnosis of ovarian, breast, prostate, bladder, pancreatic, and head and neck cancers, and also several other malignancies. Finally, problems and prospects of the approach are discussed.
Roboz
John
J
Division of Hematology-Oncology, Department of Medicine, Mount Sinai School of Medicine, New York, New York, USA.
D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D009363:Neoplasm Proteins / Q000032:analysis*; D009369:Neoplasms / Q000175:diagnosis*; D040901:Proteomics / Q000379:methods*
D009363:Neoplasm Proteins