An automated, pressure-driven sampling device for harvesting from liquid cultures for genomic and biochemical analyses.
Here we describe an automated, pressure-driven, sampling device for harvesting 10 to 30 ml samples, in replicate, with intervals as short as 10 s. Correlation between biological replicate time courses measured by microarrays was extremely high. The sampler enables sampling at intervals within the range of many important biological processes.
Aragon
Anthony D
AD
Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA.
Quiñones
Gabriel A
GA
Allen
Chris
C
Thomas
Jason
J
Roy
Sushmita
S
Davidson
George S
GS
Wentzell
Peter D
PD
Millier
Brian
B
Jaetao
Jason E
JE
Rodriguez
Angelina L
AL
Werner-Washburne
Margaret
M
D001331:Automation; D003470:Culture Media; D004867:Equipment Design; D008828:Microbiological Techniques / Q000295:instrumentation*; D020411:Oligonucleotide Array Sequence Analysis; D012331:RNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D015203:Reproducibility of Results; D015003:Yeasts / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism
D003470:Culture Media; D012331:RNA, Fungal