title: [Study on fingerprint of unfinished product of Nauclea injection].
abstract: OBJECTIVE
  To establish HPLC fingerprints of unfinished product of Nauclea injection.
  
  
  METHODS
  A high performance liquid chromatography assay was developed with an Agilent-Extend C18 column, a gradient elution:water-5% acetonitrile (10 min), 5% acetonitrile-10% acetonitrile (10-60 min), 50% acetonitrile-water (60-70 min), a 0.7 mL x min(-1) flow rate, at detection wavelength, 245 nm within a 70 min total elution time.
  
  
  RESULTS
  The RSD of relative retention time and peak area of coexist peaks meet to method requirement during the investigation of precision, stability and repeatibity trials. All of the 10 batches of samples share a high similarity.
  
  
  CONCLUSIONS
  This method can be used to control the quality of unfinished product of Nauclea injection.
authors.affiliation: Beijing Hospital, Beijing 100730, China. tao-lee-2000@sina.com
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Wei-Jian
authors.initials: WJ
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Hu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D007267:Injections; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D019666:Rubiaceae / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal