Inhibition of osteoclast differentiation by tanshinones from the root of Salvia miltiorrhiza bunge.
We screened natural products to find compounds with anti-osteoporotic potential using a coculture-based system by which osteoclast differentiation is effectively achieved. We found that methylene chloride soluble fraction of the root of Salvia miltiorrhiza Bunge (Labiatae) suppressed osteoclast differentiation. Five tanshinones, tanshinone IIA (1), tanshinone 1 (2), cryptotanshinone (3), 15,16-dihydrotanshinone I (4), and ferruginol (5) were subsequently isolated from fraction. Among the five compounds, compounds 1-4 reduced the formation of TRAP-positive multinuclear osteoclasts. These results suggest that the identified tanshinones may be useful candidates for development of therapeutic agents to treat osteoporosis and other bone-resorptive diseases.
Lee
Song-Yi
SY
College of Pharmacy, Chosun University, 375 Seosuk-dong, Dong-ku, Gwangju 501-759, Korea.
Choi
Doo-Youn
DY
Woo
Eun-Rhan
ER
D045784:Abietanes; D000818:Animals; D001854:Bone Marrow Cells; D001862:Bone Resorption / Q000517:prevention & control; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005663:Furans; D051379:Mice; D008813:Mice, Inbred ICR; D010006:Osteoblasts; D010010:Osteoclasts / Q000187:drug effects*; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts; D018517:Plant Roots / Q000737:chemistry; D011809:Quinones; D027561:Salvia miltiorrhiza
D045784:Abietanes; D004365:Drugs, Chinese Herbal; D005663:Furans; D010616:Phenanthrenes; D010936:Plant Extracts; D011809:Quinones; C021751:tanshinone; C000713095:dihydrotanshinone I; C037886:cryptotanshinone