Endothelial cell-specific expression of Cre recombinase in transgenic mice.
Endothelial cells participate in angiogenesis, vascular homeostasis, thrombosis, inflammation and vascular wall remodeling. To study the function of genes in endothelial cells using Cre-loxP system, we generated Tie2-Cre transgenic mice, in which expression of Cre recombinase is driven by Tie2 promoter. Total six founder mice carrying the Tie2-Cre transgene were identified by genomic PCR and Southern blot. The integration efficiency is 11%. In order to test the excision activity and tissue distribution of the Cre recombinase, the Tie2-Cre transgenic line was crossed with the mouse strain carrying the Smad4 conditional alleles (Smad4(Co/Co)) or the reporter line ROSA26. PCR of multiple tissue DNA from Tie2-Cre; Smad4(Co/+) mice revealed the Cre activity in all tissues containing endothelial cells. We detected pan-endothelial expression of the Cre transgene in Tie2-Cre; ROSA26 double transgenic embryos by lacZ staining. Therefore, this mouse line may serve as a valuable tool for endothelial cell lineage analyses and conditional gene ablation in endothelial cells.
Li
Wen-Long
WL
Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing 100071, China. dragonliwen@sohu.com
Cheng
Xuan
X
Tan
Xiao-Hong
XH
Zhang
Ji-Shuai
JS
Sun
Yan-Song
YS
Chen
Lin
L
Yang
Xiao
X
D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006824:Hybridization, Genetic; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D007763:Lac Operon / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated; D042787:Receptor, TIE-2 / Q000235:genetics*; D051901:Smad4 Protein / Q000235:genetics / Q000378:metabolism
C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D011506:Proteins; D022661:RNA, Untranslated; D051901:Smad4 Protein; C490810:Smad4 protein, mouse; D042787:Receptor, TIE-2; C045073:Cre recombinase; D019426:Integrases