In vivo brain imaging using a portable 3.9 gram two-photon fluorescence microendoscope.
We introduce a compact two-photon fluorescence microendoscope based on a compound gradient refractive index endoscope probe, a DC micromotor for remote adjustment of the image plane, and a flexible photonic bandgap fiber for near distortion-free delivery of ultrashort excitation pulses. The imaging head has a mass of only 3.9 g and provides micrometer-scale resolution. We used portable two-photon microendoscopy to visualize hippocampal blood vessels in the brains of live mice.
Flusberg
Benjamin A
BA
James H. Clark Center for Biomedical Engineering & Sciences, Stanford University, Stanford, California 94305-5435, USA.
Jung
Juergen C
JC
Cocker
Eric D
ED
Anderson
Erik P
EP
Schnitzer
Mark J
MJ
D000818:Animals; D019723:Endoscopes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006624:Hippocampus / Q000098:blood supply* / Q000166:cytology*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization