title: Adsorption behavior of antimicrobial peptide histatin 5 on PMMA.
abstract: Adsorption of antimicrobial peptide histatin 5 on a poly(methyl methacrylate) denture base may serve to prevent biofilm formation, leading to a reduction of denture-induced stomatitis. This study focused on adsorption behavior of histatin 5 onto PMMA surfaces modified using a cold plasma technique and the effectiveness of histatin 5 adsorption for reducing Candida albicans biofilm formation by the quartz crystal microbalance-dissipation (QCM-D) technique. PMMA spin-coated specimens were treated with oxygen (O(2)) plasma using a plasma surface modification apparatus. The amount of histatin 5 adsorbed onto the PMMA treated with O(2) plasma is more than six times greater than that adsorbed onto untreated PMMA. The degree of histatin 5 adsorption had a negative correlation with the contact angle, whereas that of zeta-potential showed no significant correlation. XPS analysis revealed that the introduction of the carboxyl and O(2) functional groups were observable on the O(2) plasma-treated PMMA. Increased surface hydrophilicity and the formation of the carboxyl could be responsible for histatin 5 adsorption on plasma-treated PMMA. There is no significant difference between histatin-adsorbed PMMA and control PMMA for C. albicans initially attached. On the contrary, the amount of C. albicans colonization on histatin-adsorbed PMMA was significantly less than the control.
authors.affiliation: Department of Dental Materials Science and Oral Health Science Center, Tokyo Dental College, 1-2-2 Masago, Chiba 261-8502, Japan. yosinari@tdc.ac.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshinari
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kato
authors.forename: Kenichi
authors.initials: K
authors.lastname: Matsuzaka
authors.forename: Tohru
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Inoue
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Oda
authors.forename: Katsuji
authors.initials: K
authors.lastname: Okuda
authors.forename: Masaki
authors.initials: M
authors.lastname: Shimono
mesh_terms: D000327:Adsorption; D000935:Antifungal Agents / Q000737:chemistry*; D018441:Biofilms; D001843:Bone Cements / Q000737:chemistry*; D002176:Candida albicans / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D054808:Histatins; D006801:Humans; D008422:Materials Testing; D010100:Oxygen / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000935:Antifungal Agents; D001843:Bone Cements; D020099:Coated Materials, Biocompatible; C068075:HTN3 protein, human; D054808:Histatins; D012471:Salivary Proteins and Peptides; D019904:Polymethyl Methacrylate; D010100:Oxygen