Use of the Diversi Lab System for species and strain differentiation of Fusarium species isolates.
Advances in molecular typing of fusariosis would facilitate the study of its epidemiology. We tested 26 such isolates by the commercially available Diversi Lab System. The system utilizes automated repetitive sequence-based PCR (rep-PCR) and web-based data analyses. rep-PCR dendrogram cluster analysis showed agreement with species sequence identification (elongation factor 1 alpha gene). Additionally, subtype differences within the same species were noted.
Healy
M
M
Bacterial Barcodes, Inc., Houston, TX 77030, USA. mhealy@bacbarcodes.com
Reece
K
K
Walton
D
D
Huong
J
J
Frye
S
S
Raad
I I
II
Kontoyiannis
D P
DP
D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D005670:Fusarium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D009181:Mycoses / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D013045:Species Specificity
D011933:Reagent Kits, Diagnostic