Genetic diversity and differentiation of Indian isolates of Phytophthora infestans as revealed by RAPD analysis.
Sixty-seven isolates of Phytophthora infestans collected from Himalayan hill regions and subtropical planes of India were characterized by RAPD markers to assess diversity and differentiation based on location of origin. Ten random decamer primers generated 161 polymorphic fragments. Association of P. infestans isolates on the dendrogram and PCO plot revealed two clear grouping based on geographical location of origin-hill isolates and plane isolates. Quantification of diversity by Shannon index of diversity analysis demonstrated that most of the diversity was present with a particular population (hill or plane) of P. infestans isolates, with 85% variation being within and 15% being between hill and plane isolates. Subtropical plane isolates of P. infestans exhibited higher variability compared to hill isolates and they were more dispersed on the PCO plot. No clear differentiation of isolates based on mating type was reflected on the dendrogram and PCO plot.
Atheya
Ila
I
Central Potato Research Institute, Shimla 171 001, India.
Singh
B P
BP
Chakrabarti
S K
SK
Pattanayak
D
D
D004247:DNA / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D005819:Genetic Markers; D014644:Genetic Variation; D015233:Models, Statistical; D010802:Phylogeny; D010838:Phytophthora / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA