Arabidopsis H+-PPase AVP1 regulates auxin-mediated organ development.
The transport of auxin controls developmental events in plants. Here, we report that in addition to maintaining vacuolar pH, the H+-pyrophosphatase, AVP1, controls auxin transport and consequently auxin-dependent development. AVP1 overexpression results in increased cell division at the onset of organ formation, hyperplasia, and increased auxin transport. In contrast, avp1-1 null mutants have severely disrupted root and shoot development and reduced auxin transport. Changes in the expression of AVP1 affect the distribution and abundance of the P-adenosine triphosphatase and Pinformed 1 auxin efflux facilitator, two proteins implicated in auxin distribution. Thus, AVP1 facilitates the auxin fluxes that regulate organogenesis.
Li
Jisheng
J
Department of Plant Science, University of Connecticut, Storrs, CT 06268, USA.
Yang
Haibing
H
Peer
Wendy Ann
WA
Richter
Gregory
G
Blakeslee
Joshua
J
Bandyopadhyay
Anindita
A
Titapiwantakun
Boosaree
B
Undurraga
Soledad
S
Khodakovskaya
Mariya
M
Richards
Elizabeth L
EL
Krizek
Beth
B
Murphy
Angus S
AS
Gilroy
Simon
S
Gaxiola
Roberto
R
D000251:Adenosine Triphosphatases / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D001692:Biological Transport; D002452:Cell Count; D049109:Cell Proliferation; D048430:Cell Shape; D002473:Cell Wall / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D017403:In Situ Hybridization; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D043564:Inorganic Pyrophosphatase / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism; D018519:Meristem / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D009154:Mutation; D018515:Plant Leaves / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D017494:Proton Pumps / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D015398:Signal Transduction; D014170:Transformation, Genetic
D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; C116363:PIN1 protein, Arabidopsis; D017494:Proton Pumps; C030737:indoleacetic acid; D000251:Adenosine Triphosphatases; C505048:AVP1 protein, Arabidopsis; D043564:Inorganic Pyrophosphatase