title: High-level production of astaxanthin by Xanthophyllomyces dendrorhous mutant JH1 using statistical experimental designs.
abstract: Medium composition was optimized for high-level production of astaxanthin by Xanthophyllomyces dendrorhous mutant JH1 using statistical experimental designs. Glucose and yeast extract were the most important factors affecting astaxanthin production. Glucose 3.89%, yeast extract 0.29%, KH2PO4 0.25%, MgSO4 0.05%, MnSO4 0.02%, and CaCl2 0.01% were optimum for high-level production of astaxanthin. Under optimized conditions, the maximum concentration of astaxanthin obtained after 7 d of cultivation was 36.06 mg/l. The concentration of astaxanthin predicted by a polynomial model was 36.16 mg/l.
authors.affiliation: Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University, South Korea.
authors.forename: Jeong-Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Seong-Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Seung-Wook
authors.initials: SW
authors.lastname: Kim
authors.forename: Hyo-Ihl
authors.initials: HI
authors.lastname: Chang
mesh_terms: D001487:Basidiomycota / Q000235:genetics*; D003470:Culture Media / Q000737:chemistry; D003627:Data Interpretation, Statistical; D005947:Glucose / Q000494:pharmacology; D015233:Models, Statistical; D009154:Mutation; D012107:Research Design; D024341:Xanthophylls; D015003:Yeasts / Q000737:chemistry; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D024341:Xanthophylls; D019207:beta Carotene; C005948:astaxanthine; D005947:Glucose