[The effects of triptolide inhibition of mouse lymphocytes activated in vitro].
OBJECTIVE
To study the effects of triptolidel on murine T lymphocytes activated.


METHODS
We isolated and cultured the mouse lymphocytes from lymph nodes of mice in vitro. The lymphocytes were pre-treated triptolide for 1 h prior to activation with ConA. After 6 hours stimulation, determined IL-2 and IFN mRNA expression by RT-PCR. CD69 expression of mouse CD3+ T cell was determined by multi-color flow cytometric analysis after 6 hours stimulation, and CD25 expression of mouse CD3+ T cell was determined by multi-color flow cytometric analysis after 24 hours stimulation.


RESULTS
TRD could inhibit IL-2 and IFN-gamma mRNA expression. It also inhibited CD69 and CD25 expression of mouse CD3+ T cell.


CONCLUSIONS
the immunosuppressive mechanism of TRD may be related to the inhibitory effects on IL-2 and IFN-gamma mRNA expression and the inhibition of CD69 and CD25 expression.
Yu
Yu
Y
Key Laboratory of Tissue Transplantation & Immunology of Ministy of Educational Specialized Laboratory, Jinan University, Guangzhou.
Zeng
Yaoying
Y
Liu
Liang
L
Ji
Yuhua
Y
Zhao
Jinxian
J
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D017252:CD3 Complex / Q000187:drug effects; D002478:Cells, Cultured; D004224:Diterpenes / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004852:Epoxy Compounds; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010616:Phenanthrenes / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D012333:RNA, Messenger / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015703:Antigens, CD; D017252:CD3 Complex; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D004852:Epoxy Compounds; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D010616:Phenanthrenes; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; C001899:triptolide; D007371:Interferon-gamma