[Effects of mechanical stress on the proliferation kinetics of the human periodontal fibroblast cells in vitro].
OBJECTIVE
The purpose of this study was to explore the possible principles for remodeling of periodontal tissues under mechanical stretching by investigating the effects of intermittent mechanical stretching delivered by a new invented type of cell stress machine on the proliferation kinetics of the human periodontal fibroblast cells (hPDLFs) in vitro.


METHODS
hPDLFs were cultured in vitro. The 4 - 7th generation of hPDLFs were undergone mechanical stretching. All cells were grouped as strained and unstrained (controls). Strained groups cells were gathered after 2, 4 and 6 hours with the stress 1 000, 2 000 and 3 000 mustrain. Flow cytometry (FCM) was used to examine the ratio of DNA (S%) and the index of proliferation(PI).


RESULTS
Mechanical force would lead to the change of hPDLFs proliferation. Within the experiment, both 1 000 mustrain and 2 000 mustrain could increase the proliferation index (PI) (P < 0.05). While under 3 000 mustrain, DNA synthesis was significantly decreased( P < 0.05).


CONCLUSIONS
Some range of mechanical stretching accelerates hPDLFs proliferation. This research discusses the remodeling process of periodontal tissues undergoing the mechanical stretching on cytological view, and it makes a basement for further revealing the mechanical strain induced periodontal biological principles. Also it provides a useful direction for clinical orthodontic therapy.
Wang
Yong
Y
Dept. of Prothodontics and Orthodontics, Guiyang Medical College, Guiyang 550004, China.
Jia
Ying
Y
Zhao
Zhi-He
ZH
Zhang
Zhong-Ping
ZP
D049109:Cell Proliferation; D002478:Cells, Cultured; D004247:DNA; D005347:Fibroblasts; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D010513:Periodontal Ligament; D013314:Stress, Mechanical
D004247:DNA