Subtype C1 persistent infection of HHV-8 in a PEL patient.
PEL, a rare type of lymphoma constituting less than 5% of NHLs, has been recently identified as a distinct clinical and pathological entity among the B-cell lymphomas, with characteristic morphologic, immunophenotypic, molecular and viral features. ICC, PCR, RT-PCR and sequencing were carried out in biologicals samples from a 44-year-old, non-smoker Caucasian male patient of Greek nationality, HIV-1 negative and HCV positive. The ICC results showed CD30 + , Vimentin + , EMA + , Ki67 + , Pankeratin- and negative to B and T antibodies. In addition, HHV-8 was detected in pleural fluid. Examination of blood samples of the patient over a period of nearly two years showed a persistent infection of HHV-8. Phylogenetic analysis revealed a close relation to the C1 variant of HHV-8. The samples was also found EBV negative by PCR. Using a combination of clinical, morphological, immunohistochemical features and molecular biology techniques in this study we document a PEL case with persistent HHV-8 of genotype C1 infection.
Metaxa-Mariatou
V
V
Molecular Biology Research Center Antonis Papayiannis, Diagnostic and Therapeutic Center HYGEIA, Kifissias Ave. & 4 Erythrou Stavrou Str., 151 23, Maroussi, Athens, Greece.
Papaioannou
D
D
Loli
A
A
Papadopoulou
I
I
Gazouli
M
M
Mavroudis
P
P
Nasioulas
G
G
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D005500:Follow-Up Studies; D005838:Genotype; D006115:Greece / Q000208:ethnology; D006566:Herpesviridae Infections / Q000150:complications* / Q000235:genetics / Q000628:therapy / Q000821:virology*; D019288:Herpesvirus 8, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006689:Hodgkin Disease; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000150:complications* / Q000235:genetics / Q000628:therapy / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
C111600:K1 protein, Human herpesvirus 8; D014764:Viral Proteins