title: [Molecular characterization of a glycopeptide-resistant Enterococcus faecalis outbreak in an intensive care unit].
abstract: BACKGROUND
  Between January and August 2003 glycopeptide-resistant Enterococcus faecalis was isolated from eight patients admitted to the intensive care unit (ICU).
  
  
  METHODS
  Antibiotic susceptibility testing was performed by disk diffusion and the Etest, clonal relatedness of the isolates was studied by pulsed-field gel electrophoresis (PFGE), and the presence of vanA was investigated by PCR.
  
  
  CONCLUSIONS
  All the isolates were vanA-positive and had an identical PFGE pattern, showing that an outbreak had occurred in our ICU.
authors.affiliation: Servicio de Microbiología, Hospital Universitario Son Dureta, Palma de Mallorca, Spain. mmaciaromero@hotmail.com
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Maciá
authors.forename: Carlos
authors.initials: C
authors.lastname: Juan
authors.forename: Antonio
authors.initials: A
authors.lastname: Oliver
authors.forename: Olga
authors.initials: O
authors.lastname: Hidalgo
authors.forename: José Luis
authors.initials: JL
authors.lastname: Pérez
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D019729:Carbon-Oxygen Ligases / Q000235:genetics; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy / Q000382:microbiology*; D006801:Humans; D007362:Intensive Care Units; D016133:Polymerase Chain Reaction; D013030:Spain; D020713:Vancomycin Resistance
chemical_list: D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; D019729:Carbon-Oxygen Ligases