Isolation of neural stem cells from damaged rat cerebral cortex after traumatic brain injury.
Nestin-positive cells were seen around the damaged area at 24 h, 72 h and 7 days after rat traumatic brain injury. Tissue was isolated from around the damaged area at 72 h after injury and spheres were cultured with basic fibroblast growth factor and epidermal growth factor. These spheres could not be isolated at 24 h and 7 days after injury. Isolated spheres consisted of nestin-positive neural stem cells. Neurospheres differentiated into Tuj1-positive, glial fibrillary acidic protein-positive and O4-positive cells after 4 days in culture without basic fibroblast growth factor and epidermal growth factor. These results indicate that isolated and cultured neurospheres can differentiate into neurons and glia. An increase in nestin-positive cells around a cerebral cortical damaged area might contribute to neurogenesis and neuroplasticity.
Itoh
Tatsuki
T
Department of Pathology, Kinki University School of Medicine, Osaka, Japan. tatsuki@med.kindai.ac.jp
Satou
Takao
T
Hashimoto
Shigeo
S
Ito
Hiroyuki
H
D000818:Animals; D001930:Brain Injuries / Q000473:pathology*; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000096:biosynthesis / Q000378:metabolism; D008297:Male; D008858:Microscopy, Phase-Contrast; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000378:metabolism; D064231:Nestin; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000502:physiology*
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin