title: CRF and urocortin differentially modulate GluRdelta2 expression and distribution in parallel fiber-Purkinje cell synapses.
abstract: Corticotropin-releasing factor (CRF) and urocortin (UCN) are closely related multifunctional regulators, governing, among other processes, Purkinje cell development. Here, we investigate the effects of CRF and UCN on Purkinje cells in organotypic slices. We show that both peptides upregulate delta2 ionotropic glutamate receptor gene expression, and increase the abundance of the receptor in the postsynaptic density. However, only UCN treatment results in increased delta2 protein level per Purkinje cell, implying the existence of posttranscriptional regulation of GluRdelta2 mRNA. CRF, in contrast, reduces the number of delta2-positive dendritic shafts per cell, implying that the increase of GluRdelta2 in remaining synapses may be mainly due to its retargeting. We further observed different patterns of GluRdelta2 distribution in the zone of postsynaptic density upon CRF and UCN treatment. CRF treatment results in a clustered distribution of GluRdelta2 along the postsynaptic density, whereas UCN treatment provides a linear distribution.
authors.affiliation: Department of Cell Biology, Laboratory for Electron Microscopy, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Gounko
authors.forename: Vasily
authors.initials: V
authors.lastname: Rybakin
authors.forename: Dharamdajal
authors.initials: D
authors.lastname: Kalicharan
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Siskova
authors.forename: Albert
authors.initials: A
authors.lastname: Gramsbergen
authors.forename: Johannes J L
authors.initials: JJ
authors.lastname: van der Want
mesh_terms: D000818:Animals; D002525:Cerebellar Cortex / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism* / Q000494:pharmacology; D049229:Dendritic Spines / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D018698:Glutamic Acid / Q000378:metabolism; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology; D009924:Organ Culture Techniques; D017729:Presynaptic Terminals / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D011499:Protein Processing, Post-Translational / Q000187:drug effects / Q000502:physiology; D011689:Purkinje Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017470:Receptors, Glutamate / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013570:Synaptic Membranes / Q000187:drug effects / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology; D054832:Urocortins
chemical_list: D012333:RNA, Messenger; D017470:Receptors, Glutamate; D054832:Urocortins; C107913:glutamate receptor delta 2; D018698:Glutamic Acid; D003346:Corticotropin-Releasing Hormone