Sustained release of bovine serum albumin using implantable wafers prepared by MPEG-PLGA diblock copolymers.
MPEG-PLGA diblock copolymers, consisting of methoxy polyethylene glycol (MPEG) and poly(L-lactic-co-glycolic acid) (PLGA), were synthesized by ring-opening polymerization of L-lactide and glycolide in the presence of MPEG as an initiator. Implantable wafers, using diblock copolymers as a drug carrier, were fabricated by direct compression method after freeze milling of the diblock copolymers and bovine serum albumin-fluorescein isothiocyanate (BSA-FITC) as a model protein drug. The wafers prepared with MPEG-PLGA diblock copolymers exhibited initial burst in the release of BSA. The BSA release profiles from the wafers depended on MPEG-PLGA diblock copolymer compositions. The in vitro release of the BSA also correlated with the degradation rate of the PLGA part in the diblock polymers. The wafers prepared from diblock copolymers with an increased MPEG segment showed the more structural metamorphosis of crack form due to higher water absorption of MPEG inside the wafer, and induced faster BSA release. The wafers prepared by using MPEG-PLGA diblock copolymers in the presence of small intestinal submucosa (SIS) as a drug carrier additive exhibited controlled BSA release profiles, although the wafers exhibited release patterns with a lag time at the initial stage as the MPEG segment in diblock copolymer compositions increased. Thus, we confirmed that the MPEG-PLGA diblock copolymers could be used as a protein delivery carrier in implantable wafer form.
Kim
Moon Suk
MS
Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O. Box 107, 100 Jang-dong, Yuseong-gu, Daejeon 305-600, Republic of Korea. mskim@krict.re.kr
Seo
Kwang Su
KS
Hyun
Hoon
H
Kim
Sun Kyung
SK
Khang
Gilson
G
Lee
Hai Bang
HB
D020341:Absorbable Implants; D000818:Animals; D002417:Cattle; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D004343:Drug Implants; D007700:Kinetics; D008970:Molecular Weight; D011091:Polyesters; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011098:Polyglactin 910 / Q000138:chemical synthesis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry*; D012995:Solubility
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004343:Drug Implants; D011091:Polyesters; C558447:methoxypolyethyleneglycol-poly(lactic-co-glycolic acid); D012710:Serum Albumin, Bovine; D011098:Polyglactin 910; D011092:Polyethylene Glycols