title: parC mutations in fluoroquinolone-resistant Borrelia burgdorferi.
abstract: We have isolated in vitro fluoroquinolone-resistant mutants of the Lyme disease agent, Borrelia burgdorferi. Mutations in parC, which encodes a subunit of topoisomerase IV, were associated with loss of susceptibility to sparfloxacin, moxifloxacin, and Bay-Y3118, but not ciprofloxacin. This is the first description of fluoroquinolone resistance in the spirochete phylum.
authors.affiliation: Division of Biological Sciences, The University of Montana, Missoula, 59812-4824, USA.
authors.forename: Kendal M
authors.initials: KM
authors.lastname: Galbraith
authors.forename: Amanda C
authors.initials: AC
authors.lastname: Ng
authors.forename: Betsy J
authors.initials: BJ
authors.lastname: Eggers
authors.forename: Craig R
authors.initials: CR
authors.lastname: Kuchel
authors.forename: Christian H
authors.initials: CH
authors.lastname: Eggers
authors.forename: D Scott
authors.initials: DS
authors.lastname: Samuels
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D025065:Borrelia burgdorferi / Q000235:genetics*; D004269:DNA, Bacterial; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones / Q000494:pharmacology*; D005798:Genes, Bacterial; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D024841:Fluoroquinolones