[New type pathogenic mycoplasmas isolated from female patients with genital infection in Nanjing, China].
OBJECTIVE
To study if infection of new type pathogenic mycoplasmas, Mycoplasma penetrans (Mpe), Mycoplasma fermentans (Mf), and Mycoplasma pirum (Mpi), exists in the genital tract of gynecologic patients with genital infection in China so as to provide information for clinical diagnosis and therapy.


METHODS
Cervical secretions from 172 patients with infectious diaseses of gentital tract and 172 healthy controls were collected and inoculated in improved SP-4 culture medium. Biochemical reaction, coagglutination test, metabolism-inhibition test, polymerase chain reaction (PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms.


RESULTS
One strain of Mycoplasma penetrans (0.58%) and two strains of Mycoplasma fermentans (1.16%) were isolated from the cervical secretions of patients, but none was found in healthy controls.


CONCLUSIONS
Mpe infection and Mf infection exist in female patients with genital infection in Nanjing.
Ren
M
M
Department of Obstetrics and Gynecology, Affiliated Hospital of Southeast University, Nanjing 210009, China.
Zhao
J
J
Li
H
H
D000328:Adult; D005260:Female; D006801:Humans; D008875:Middle Aged; D009175:Mycoplasma Infections / Q000382:microbiology*; D016858:Mycoplasma fermentans / Q000302:isolation & purification*; D018661:Mycoplasma penetrans / Q000302:isolation & purification*; D002575:Uterine Cervicitis / Q000382:microbiology*; D014627:Vaginitis / Q000382:microbiology*