Measles immunity testing: comparison of two measles IgG ELISAs with plaque reduction neutralisation assay.
Two commercial IgG ELISAs, one based on recombinant nucleocapsid antigen and one based on cell culture grown native virus antigens, were evaluated for measles immunity testing by comparison with plaque reduction neutralisation test (PRNT). Qualitative results of the two ELISAs showed 92% agreement with those of PRNT. The sensitivity of the two ELISAs was 89.6%. False negative ELISA results were obtained in 10% of sera, mainly sera containing low levels of neutralising antibody. The specificity of both ELISAs was 100%. Measles IgG ELISAs perform adequately for immunity testing, correctly identifying seronegative individuals for vaccination.
Cohen
B J
BJ
Virus Reference Department, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK. Bernard.cohen@hpa.org.uk
Parry
R P
RP
Doblas
D
D
Samuel
D
D
Warrener
L
L
Andrews
N
N
Brown
D
D
D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005188:False Negative Reactions; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008457:Measles / Q000276:immunology*; D008459:Measles virus / Q000276:immunology*; D009500:Neutralization Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D010948:Viral Plaque Assay
D000914:Antibodies, Viral; D007074:Immunoglobulin G