title: Detection of Chlamydia pneumoniae and Helicobacter pylori in atherosclerotic plaques of carotid artery by polymerase chain reaction.
abstract: OBJECTIVE
  A possible role of some microorganisms has been proposed in the pathogenesis of atherosclerosis, but it is still an unresolved issue. We investigated the presence of Chlamydia pneumoniae and Helicobacter pylori DNA in carotid artery atherosclerotic plaques by using PCR.
  
  
  METHODS
  One hundred and four patients with atherosclerotic diseases were included. The study group consisted of 52 atherosclerotic plaque specimens obtained from the carotid arteries of patients who had carotid endarterectomy and the control group consisted of 52 specimens obtained from the macroscopically healthy regions of ascending aorta in patients who had undergone coronary artery bypass grafting. The presence of C. pneumoniae and H. pylori DNA in endarterectomy specimens were demonstrated by PCR.
  
  
  RESULTS
  C. pneumoniae DNA was detected in 16 of 52 (30.8%) atherosclerotic plaques and 1 of 52 (1.9%) macroscopically healthy ascending aorta wall specimens (P < 0.001). H. pylori DNA was detected in 9 of 52 (17.3%) atherosclerotic plaques and none of the controls (P = 0.003).
  
  
  CONCLUSIONS
  The higher incidence of C. pneumoniae and H. pylori DNA in atherosclerotic plaques suggests that these microorganisms may play a role in the pathogenesis of atherogenesis.
authors.affiliation: Department of Cardiovascular Surgery, Siyami Ersek Thoracic and Cardiovascular Surgery Center, Istanbul, Turkey. mehmetkaplan@superonline.com
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kaplan
authors.forename: Serap Simsek
authors.initials: SS
authors.lastname: Yavuz
authors.forename: Bayer
authors.initials: B
authors.lastname: Cinar
authors.forename: Vedat
authors.initials: V
authors.lastname: Koksal
authors.forename: Mustafa Sinan
authors.initials: MS
authors.lastname: Kut
authors.forename: Fikri
authors.initials: F
authors.lastname: Yapici
authors.forename: Hakan
authors.initials: H
authors.lastname: Gercekoglu
authors.forename: Mahmut Murat
authors.initials: MM
authors.lastname: Demirtas
mesh_terms: D000368:Aged; D002339:Carotid Arteries / Q000382:microbiology*; D002340:Carotid Artery Diseases / Q000382:microbiology* / Q000601:surgery; D016022:Case-Control Studies; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D016894:Endarterectomy, Carotid; D005260:Female; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004269:DNA, Bacterial