Studies on the use of NE-4C embryonic neuroectodermal stem cells for targeting brain tumour.
Neural stem cells were suggested to migrate to and invade intracranial gliomas. In the presented studies, interactions of NE-4C embryonic neural stem cells were investigated with C6 and Gl261, LL and U87, glioblastoma cells or with primary astrocytes. Glioma-derived humoral factors did not influence the proliferation of stem cells. NE-4C-derived humoral factors did not alter the proliferation of Gl261 and U87 cells, but increased the mitotic activity of C6 cells and that of astrocytes. In chimera-aggregates, all types of glioma cells co-aggregated with astrocytes, but most of them segregated from stem cells. Complete intercalation of stem and tumour cells was detected only in chimera-aggregates of Gl261 glioma and NE-4C cells. If mixed suspensions of NE-4C and Gl261 cells were injected into the brain, stem cells survived and grew inside the tumour mass. NE-4C stem cells, however, did not migrate towards the tumour, if implanted near to Gl261 tumours established in the adult mouse forebrain. The observations indicate that not all types of stem cells could be used for targeting all sorts of brain tumours.
Demeter
Kornél
K
Institute of Experimental Medicine of Hungarian Academy of Sciences, Laboratory of Neural Cell Biology, Szigony u. 43, H-1083 Budapest, Hungary.
Zádori
Anita
A
Agoston
Viktor A
VA
Madarász
Emília
E
D000818:Animals; D001253:Astrocytes / Q000502:physiology; D001932:Brain Neoplasms / Q000503:physiopathology / Q000628:therapy*; D016380:Brain Tissue Transplantation / Q000379:methods*; D002449:Cell Aggregation / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002460:Cell Line; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D004475:Ectoderm / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D005909:Glioblastoma / Q000503:physiopathology / Q000628:therapy; D006085:Graft Survival / Q000502:physiology; D006133:Growth Substances / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D051379:Mice; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D051381:Rats; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D006133:Growth Substances