title: Use of dual TaqMan probes to increase the sensitivity of 1-step quantitative reverse transcription-PCR: application to the detection of SARS coronavirus.
authors.affiliation: Department of Health Technology & Informatics, The Hong Kong Polytechnic University, Hong Kong SAR, China. shea.ping.yip@polyu.edu.hk
authors.forename: Shea Ping
authors.initials: SP
authors.lastname: Yip
authors.forename: Shing Shun T
authors.initials: SS
authors.lastname: To
authors.forename: Polly H M
authors.initials: PH
authors.lastname: Leung
authors.forename: Tsz Shan
authors.initials: TS
authors.lastname: Cheung
authors.forename: Peter K C
authors.initials: PK
authors.lastname: Cheng
authors.forename: Wilina W L
authors.initials: WW
authors.lastname: Lim
mesh_terms: D015342:DNA Probes; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D019914:Taq Polymerase
chemical_list: D015342:DNA Probes; D019914:Taq Polymerase