Summer heat stress alters the mRNA expression of selective-uptake and endocytotic receptors in bovine ovarian cells.
Summer heat stress (HS) is a major factor in decreased reproductive performance in high-producing dairy cattle, possibly by affecting the steroidogenic capacity of ovarian follicles and ovarian follicular dynamics. In the present study, mRNA expression of cholesterol receptors was determined in bovine ovarian cells. Two endocytotic receptors (very-low-density lipoprotein receptor (VLDLr) and low-density lipoprotein receptor (LDLr)), and two selective-uptake receptors (scavenger receptor class B type 1 receptor (SRB1) and the lipoprotein-receptor-related protein 8 (LRP8)) were evaluated. Ovarian follicles in four diameter categories were evaluated from cows under non-heat stress (NHS) and HS conditions. As follicle size increased, expression of mRNA in NHS cows increased for the selective-uptake receptors, SRB1 and LRP8, and decreased (P<0.004) for the endocytotic receptors, LDLr and VLDLr. In contrast, in HS cows, mRNA expression did not significantly change (with increasing follicle diameter) for either receptor type. With increasing follicle diameter, cholesterol and fatty acid concentrations in the follicular fluid did not change in HS cows, whereas in NHS cows, cholesterol increased (P<0.008) and fatty acid decreased (P<0.0001). These changes paralleled those in the different lipoprotein fractions in the follicular fluid. In follicles from HS cows, the altered mRNA expression patterns for the endocytotic and selective-uptake receptors caused changes in the regulation of cholesterol supply at critical stages of folliculogenesis, which may play a role in the low turnover rates of ovarian follicles during the summer.
Argov
Nurit
N
Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, P.O. Box 12, Hertzel Street, Rehovot 76-100, Israel. argov@agri.huji.ac.il
Moallem
Uzi
U
Sklan
David
D
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism*; D002784:Cholesterol / Q000032:analysis; D004705:Endocytosis / Q000235:genetics*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry*; D015870:Gene Expression / Q000502:physiology*; D018882:Heat Stress Disorders / Q000378:metabolism / Q000662:veterinary*; D026502:LDL-Receptor Related Proteins; D008074:Lipoproteins / Q000032:analysis; D010053:Ovary / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D011973:Receptors, LDL / Q000235:genetics; D018110:Receptors, Lipoprotein / Q000235:genetics; D051122:Scavenger Receptors, Class B / Q000235:genetics; D012621:Seasons
D005227:Fatty Acids; D026502:LDL-Receptor Related Proteins; D008074:Lipoproteins; D012333:RNA, Messenger; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; D051122:Scavenger Receptors, Class B; C036053:VLDL receptor; C098773:low density lipoprotein receptor-related protein 8; D002784:Cholesterol