Molecular cloning and characterization of a novel mannose-binding lectin cDNA from Zantedeschia aethiopica.
Using RNA extracted from Zantedeschia aethiopica young leaves and primers designed according to the conservative regions of Araceae lectins, the full-length cDNA of Z. aethiopica agglutinin (ZAA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of zaa was 871 bp and contained a 417 bp open reading frame (ORF) encoding a lectin precursor of 138 amino acids. Through comparative analysis of zaa gene and its deduced amino acid sequence with those of other Araceae species, it was found that zaa encoded a precursor lectin with signal peptide. Secondary and three-dimensional structure analyses showed that ZAA had many common characters of mannose-binding lectin superfamily and ZAA was a mannose-binding lectin with three mannose-binding sites. Southern blot analysis of the genomic DNA revealed that zaa belonged to a multi-copy gene family.
Chen
Zhonghai
Z
State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Pang
Yongzhen
Y
Liu
Xiaojun
X
Wang
Xinglong
X
Deng
Zhongxiang
Z
Sun
Xiaofen
X
Tang
Kexuan
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008358:Mannose / Q000737:chemistry; D037601:Mannose-Binding Lectin / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010944:Plants; D011487:Protein Conformation; D021382:Protein Sorting Signals; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D031064:Zantedeschia / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D037102:Lectins; D037601:Mannose-Binding Lectin; D021382:Protein Sorting Signals; D012313:RNA; D008358:Mannose