title: Closing microvascular lesions with fibrin sealant-attached muscle pads.
abstract: Fibrin sealants are used in a variety of surgical procedures, mainly for purposes of hemostasis and assisted wound healing. The combined use of fibrin sealant and autologous muscle pads for hemostasis was not reported previously. Arterial incisions in the common carotid artery in rats were closed by the combined application of fibrin sealant and an autologous muscle pad. Postsurgical vessel patency and degree of stenosis were evaluated by color duplex sonography, computed tomography angiography, and postmortem histology. The combined application of muscle pad and fibrin sealant and achievement of hemostasis was feasible in all animals. Seventy-eight percent of animals showed no or only slight postsurgical vessel stenosis. Our method is simple and quick to perform, showing a high potential for hemostasis in microvascular lesions. Therefore, it might be used in future experimental studies for conservation of vessel patency after arterial catheterization and in experimental or clinical vascular surgery.
authors.affiliation: Department of Neurology, University of Regensburg, Regensburg, Germany. Nando.Fehm@medbo.de
authors.forename: Nando Percy
authors.initials: NP
authors.lastname: Fehm
authors.forename: Bijan
authors.initials: B
authors.lastname: Vatankhah
authors.forename: Michael S
authors.initials: MS
authors.lastname: Dittmar
authors.forename: Yesim
authors.initials: Y
authors.lastname: Tevetoglu
authors.forename: Gerald
authors.initials: G
authors.lastname: Retzl
authors.forename: Markus
authors.initials: M
authors.lastname: Horn
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D001707:Biopsy, Needle; D016063:Blood Loss, Surgical / Q000517:prevention & control*; D002339:Carotid Arteries / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D006488:Hemostasis, Surgical / Q000379:methods; D007150:Immunohistochemistry; D018810:Magnetic Resonance Angiography; D008297:Male; D008866:Microsurgery / Q000379:methods*; D018482:Muscle, Skeletal / Q000637:transplantation; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013524:Surgical Flaps / Q000098:blood supply*; D018608:Ultrasonography, Doppler
chemical_list: D015718:Fibrin Tissue Adhesive