title: Investigation of protein functions through data-mining on integrated human transcriptome database, H-Invitational database (H-InvDB).
abstract: H-Invitational Database (H-InvDB; ) is a human transcriptome database, containing integrative annotation of 41,118 full-length cDNA clones originated from 21,037 loci. H-InvDB is a product of the H-Invitational project, an international collaboration to systematically and functionally validate human genes by analysis of a unique set of high quality full-length cDNA clones using automatic annotation and human curation under unified criteria. Here, 19,574 proteins encoded by these cDNAs were classified into 11,709 function-known and 7865 function-unknown hypothetical proteins by similarity with protein databases and motif prediction (InterProScan). The proportion of "hypothetical proteins" in H-InvDB was as high as 40.4%. In this study, we thus conducted data-mining in H-InvDB with the aim of assigning advanced functional annotations to those hypothetical proteins. First, by data-mining in the H-InvDB version of GTOP, we identified 337 SCOP domains within 7865 H-Inv hypothetical proteins. Second, by data-mining of predicted subcellular localization by SOSUI and TMHMM in H-InvDB, we found 1032 transmembrane proteins within H-Inv hypothetical proteins. These results clearly demonstrate that structural prediction is effective for functional annotation of proteins with unknown functions. All the data in H-InvDB are shown in two main views, the cDNA view and the Locus view, and five auxiliary databases with web-based viewers; DiseaseInfo Viewer, H-ANGEL, Clustering Viewer, G-integra and TOPO Viewer; the data also are provided as flat files and XML files. The data consists of descriptions of their gene structures, novel alternative splicing isoforms, functional RNAs, functional domains, subcellular localizations, metabolic pathways, predictions of protein 3D structure, mapping of SNPs and microsatellite repeat motifs in relation with orphan diseases, gene expression profiling, and comparisons with mouse full-length cDNAs in the context of molecular evolution. This unique integrative platform for conducting in silico data-mining represents a substantial contribution to resources required for the exploration of human biology and pathology.
authors.affiliation: Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, AIST Waterfront Bio-IT Research Building, 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan.
authors.forename: Chisato
authors.initials: C
authors.lastname: Yamasaki
authors.forename: Kanako O
authors.initials: KO
authors.lastname: Koyanagi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Fujii
authors.forename: Takeshi
authors.initials: T
authors.lastname: Itoh
authors.forename: Roberto
authors.initials: R
authors.lastname: Barrero
authors.forename: Takuro
authors.initials: T
authors.lastname: Tamura
authors.forename: Yumi
authors.initials: Y
authors.lastname: Yamaguchi-Kabata
authors.forename: Motohiko
authors.initials: M
authors.lastname: Tanino
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Takeda
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukuchi
authors.forename: Satoru
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nomura
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Tadashi
authors.initials: T
authors.lastname: Imanishi
authors.forename: Takashi
authors.initials: T
authors.lastname: Gojobori
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D030561:Databases, Nucleic Acid; D015894:Genome, Human; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D011506:Proteins