title: Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs.
abstract: The objective was to determine relationships among classical semen characteristics, sperm chromatin structure assay (SCSA), lipid peroxidation and antioxidant enzymatic activity in ram-lamb semen. Fifty-seven ram-lambs were electroejaculated, and routine semen evaluation was conducted (as part of a breeding soundness evaluation). The percentage of sperm DNA fragmentation index (%DFI) and the percentage of sperm with abnormally high DNA stainability (HDS; immature spermatozoa) were determined by SCSA using the metachromatic properties of acridine orange. Semen was centrifuged at 800 x g for 15 min to separate spermatozoa and seminal plasma and the aliquots were stored at -70 degrees C until analyzed. Lipid peroxidation, superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in seminal plasma and spermatozoa were measured by spectrophotometric assays. The classical semen parameters were negatively related to lipid peroxidation and GPx activity in spermatozoa; motility and morphology were negatively related to %DFI (P < 0.05). Based on Kruskal-Wallis pair-wise comparison of median values among breeding soundness outcome groups, %DFI was lower in the satisfactory group compared to other groups (P < 0.05) and the lipid peroxidation and GPx activity in seminal plasma and spermatozoa were lower in satisfactory and questionable groups (P < 0.05). However, the SOD was lower in the unsatisfactory group (P < 0.05). In summary, classical semen parameters were negatively related to % DFI, lipid peroxidation and GPx activity in ram-lamb spermatozoa and seminal plasma. There were indications that SOD and GPx have crucial protective roles against the toxic effect of reactive oxygen species (ROS) in ram-lamb semen.
authors.affiliation: Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061, USA. drram@vt.edu
authors.forename: Ramanathan
authors.initials: R
authors.lastname: Kasimanickam
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Pelzer
authors.forename: Vanmathy
authors.initials: V
authors.lastname: Kasimanickam
authors.forename: William S
authors.initials: WS
authors.lastname: Swecker
authors.forename: Craig D
authors.initials: CD
authors.lastname: Thatcher
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002843:Chromatin / Q000648:ultrastructure; D053938:DNA Fragmentation; D005979:Glutathione Peroxidase / Q000032:analysis; D015227:Lipid Peroxidation; D016015:Logistic Models; D008297:Male; D012661:Semen / Q000201:enzymology / Q000502:physiology*; D012756:Sheep; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry / Q000201:enzymology* / Q000648:ultrastructure; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D000975:Antioxidants; D002843:Chromatin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase