title: Pyrimidine nucleoside salvage confers an advantage to Xenorhabdus nematophila in its host interactions.
abstract: Xenorhabdus nematophila is a mutualist of entomopathogenic nematodes and a pathogen of insects. To begin to examine the role of pyrimidine salvage in nutrient exchange between X. nematophila and its hosts, we identified and mutated an X. nematophila tdk homologue. X. nematophila tdk mutant strains had reduced virulence toward Manduca sexta insects and a competitive defect for nematode colonization in plate-based assays. Provision of a wild-type tdk allele in trans corrected the defects of the mutant strain. As in Escherichia coli, X. nematophila tdk encodes a deoxythymidine kinase, which converts salvaged deoxythymidine and deoxyuridine nucleosides to their respective nucleotide forms. Thus, nucleoside salvage may confer a competitive advantage to X. nematophila in the nematode intestine and be important for normal entomopathogenicity.
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Samantha S
authors.initials: SS
authors.lastname: Orchard
authors.forename: Heidi
authors.initials: H
authors.lastname: Goodrich-Blair
mesh_terms: D000818:Animals; D003857:Deoxyuridine / Q000378:metabolism; D018402:Manduca / Q000382:microbiology*; D011741:Pyrimidine Nucleosides / Q000378:metabolism*; D017168:Rhabditida / Q000382:microbiology*; D013559:Symbiosis; D013936:Thymidine / Q000378:metabolism; D013937:Thymidine Kinase / Q000235:genetics / Q000378:metabolism; D014774:Virulence; D020593:Xenorhabdus / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*
chemical_list: D011741:Pyrimidine Nucleosides; D013937:Thymidine Kinase; D013936:Thymidine; D003857:Deoxyuridine