Biosynthesis of active Bacillus subtilis urease in the absence of known urease accessory proteins.
Bacillus subtilis contains urease structural genes but lacks the accessory genes typically required for GTP-dependent incorporation of nickel. Nevertheless, B. subtilis was shown to possess a functional urease, and the recombinant enzyme conferred low levels of nickel-dependent activity to Escherichia coli. Additional investigations of the system lead to the suggestion that B. subtilis may use unidentified accessory proteins for in vivo urease activation.
Kim
Jong Kyong
JK
Cell and Molecular Biology Program, Michigan State University, East Lansing, Michigan 48824-4320, USA.
Mulrooney
Scott B
SB
Hausinger
Robert P
RP
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism; D004789:Enzyme Activation; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006160:Guanosine Triphosphate / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D007709:Klebsiella / Q000201:enzymology / Q000235:genetics; D009532:Nickel / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014510:Urease / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D011994:Recombinant Proteins; D009532:Nickel; D006160:Guanosine Triphosphate; D006867:Hydrolases; D014510:Urease