Analysis of the essential oil from radix bupleuri using capillary gas chromatography.
A simple and rapid capillary gas chromatographic (CGC) method with flame ionization detection has been newly developed for analysis of the essential oil from Radix Bupleuri. Twenty components were identified with gas chromatography-mass spectrometry. E-2-heptenal, furan, 2-pentyl, and E-2-nonenal were quantified simultaneously using the internal standard method. Decane was used as an internal standard. Separation and quantification were achieved on a DB-5 capillary column (30 m x 0.25 mm i. d., 0.25-microm film thickness). The oven temperature was programmed as follows: 60 degrees C to 70 degrees C at 1 degree C/min rate, 70 degrees C for 10 min, 3 degrees C/min to 120 degrees C, 20 degrees C/min to 250 degrees C, and held at 250 degrees C for 5 min. The oven pressure was programmed as follows: 46.1 kPa for 25 min, 20.0 kPa/min to 77.6 kPa, and then held for 22 min. Split injection was conducted with a split ratio of 10:1; flow-rate, 1.00 ml/min; carrier gas, nitrogen; injector temperature, 280 degrees C; and detector temperature, 280 degrees C. The system proved effective in resolving E-2-heptenal, furan, 2-pentyl, and E-2-nonenal peaks from their interfering components. The method displayed excellent linearity in the range of 26.8-1072 microg/ml (E-2-heptenal), 6.5-1292 microg/ml (furan, 2-pentyl), and 7.8-1564 microg/ml (E-2-nonenal). The average recovery rates of E-2-heptenal, furan, 2-pentyl, and E-2-nonenal were 100.3%, 102.8%, and 97%, respectively. CGC is a quick and accurate method for analysis of the essential oil from Radix Bupleuri.
Li
Xiuqin
X
School of Pharmacy, Shenyang Pharmaceutical University, Liaoning Province, China.
Jia
Ying
Y
Song
Aihua
A
Chen
Xiaohui
X
Bi
Kaishun
K
D029972:Bupleurum / Q000737:chemistry*; D002849:Chromatography, Gas / Q000379:methods*; D005410:Flame Ionization; D008401:Gas Chromatography-Mass Spectrometry; D009822:Oils, Volatile / Q000032:analysis*; D013696:Temperature
D009822:Oils, Volatile