title: Experimental transmission of West Nile virus (Flaviviridae: Flavivirus) by Carios capensis ticks from North America.
abstract: Seabird soft ticks, Carios capensis (Ixodida: Argasidae), originally collected from coastal Georgia, USA, were allowed to ingest a blood meal from pekin ducklings (Anas domesticus) infected with WNV. After 35 days of extrinsic incubation, the ticks transmitted virus to naive ducklings. WNV was detected via plaque assay and RTPCR in ticks and in tissues and serum of ducklings 7 days post infestation.
authors.affiliation: Department of Bioagricultural Sciences and Pest Management, Arthropod-borne and Infectious Diseases Laboratory (AIDL), Colorado State University (CSU), Fort Collins, Colorado 80523-1692, USA. joel.hutcheson@colostate.edu
authors.forename: H Joel
authors.initials: HJ
authors.lastname: Hutcheson
authors.forename: Christopher H
authors.initials: CH
authors.lastname: Gorham
authors.forename: Carlos
authors.initials: C
authors.lastname: Machain-Williams
authors.forename: Maria A
authors.initials: MA
authors.lastname: Loroño-Pino
authors.forename: Angela M
authors.initials: AM
authors.lastname: James
authors.forename: Nicole L
authors.initials: NL
authors.lastname: Marlenee
authors.forename: Bradford
authors.initials: B
authors.lastname: Winn
authors.forename: Barry J
authors.initials: BJ
authors.lastname: Beaty
authors.forename: Carol D
authors.initials: CD
authors.lastname: Blair
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000821:virology*; D001715:Bird Diseases / Q000469:parasitology / Q000635:transmission*; D004372:Ducks / Q000469:parasitology; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013987:Ticks / Q000821:virology*; D014766:Viremia / Q000662:veterinary; D014901:West Nile Fever / Q000469:parasitology / Q000635:transmission*; D014902:West Nile virus / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral