title: Limited polymorphism in the dihydropteroate synthetase gene (dhps) of Plasmodium vivax isolates from Thailand.
abstract: The dhps sequences of 55 Plasmodium vivax isolates (39 from Thailand and 16 from elsewhere) revealed mutant Pvdhps at codons 383 and/or 553 (A --> G) in 33 isolates, all from Thailand. Mutations of Pvdhps and Pvdhfr were correlated. Multiple mutations were associated with high-grade sulfadoxine-pyrimethamine resistance.
authors.affiliation: Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Mallika
authors.initials: M
authors.lastname: Imwong
authors.forename: Sasithon
authors.initials: S
authors.lastname: Pukrittayakamee
authors.forename: Qin
authors.initials: Q
authors.lastname: Cheng
authors.forename: Catrin
authors.initials: C
authors.lastname: Moore
authors.forename: Sornchai
authors.initials: S
authors.lastname: Looareesuwan
authors.forename: Georges
authors.initials: G
authors.lastname: Snounou
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: White
authors.forename: Nicholas P J
authors.initials: NP
authors.lastname: Day
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology / Q000627:therapeutic use; D003062:Codon; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D004094:Dihydropteroate Synthase / Q000737:chemistry / Q000235:genetics*; D004338:Drug Combinations; D004351:Drug Resistance; D017125:Genes, Protozoan; D006239:Haplotypes; D016780:Malaria, Vivax / Q000188:drug therapy / Q000201:enzymology / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques; D010966:Plasmodium vivax / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015995:Prevalence; D017434:Protein Structure, Tertiary; D011739:Pyrimethamine / Q000494:pharmacology / Q000627:therapeutic use; D013413:Sulfadoxine / Q000494:pharmacology / Q000627:therapeutic use; D013785:Thailand / Q000453:epidemiology
chemical_list: D000962:Antimalarials; D003062:Codon; D016054:DNA, Protozoan; D004338:Drug Combinations; D013413:Sulfadoxine; D004094:Dihydropteroate Synthase; D011739:Pyrimethamine