[Effects of Shenggu injection on mRNA expression of vascular endothelia growth factor in rat osteoblasts in vitro].
OBJECTIVE
To study the effects of Shenggu injection (SGI) on mRNA expression of vascular endothelia growth factor (VEGF) in rat osteoblasts in vitro and to explore its possible molecular mechanisms in promoting fracture healing.


METHODS
Rat osteoblasts cultured in vitro were stimulated with SGI according to the protocol. The expression levels of VEGF mRNA in the cells in every group were examined by reverse-transcriptase ploymerase chain reaction (RT-PCR).


RESULTS
When osteoblasts were stimulated with different concentrations of SGI for 5 days, the expression of VEGF mRNA peaked with 1 mg/ml SGI on the 5th day. When treated with 1 mg/ml SGI from the 1st to the 5th day, the expression of VEGF mRNA increased gradually with the increase of culturing time.


CONCLUSIONS
SGI could promote significantly the expression of VEGF mRNA in rat osteoblasts in vitro. The levels of expression of VEGF mRNA changed along with different concentrations and stimulating time of SGI.
Cheng
Jie
J
Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. chengjiecjyy@yahoo.com.cn
Peng
Rui
R
Yu
Yuan
Y
Wang
Yong-qing
YQ
Zou
Yang
Y
Xia
Ren-yun
RY
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D017102:Fracture Healing / Q000187:drug effects; D066298:In Vitro Techniques; D008516:Medicine, Chinese Traditional; D010006:Osteoblasts / Q000187:drug effects*; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A