title: Expression patterns of the DAZ-associated protein DAZAP1 in rat and human ovaries.
abstract: OBJECTIVE
  To evaluate the expression of DAZAP1 (deleted in azoospermia-associated protein 1) in rat and human ovaries.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Twelve corpus luteum (CL) specimens were collected during operation, either by laparoscopic surgery for CL rupture or by laparotomy for benign gynecologic conditions.
  
  
  METHODS
  Surgical excision of 12 human CL.
  
  
  METHODS
  Proteins analyzed by immunohistochemical staining, Western blotting, and co-immunoprecipitation experiments.
  
  
  RESULTS
  DAZAP1 is expressed in rat and human luteal cells. Expression of DAZAP1 decreases with advancing stages of CL. Co-immunoprecipitation experiments show in vivo interaction of DAZ-like (DAZL) protein with DAZAP1 in the ovarian tissues.
  
  
  CONCLUSIONS
  The expression patterns of DAZAP1 and DAZL are identical within rat and human ovaries. In mammalian species, DAZAP1 may be involved in diverse reproductive functions, ranging from cell cycle regulation and maturation of oocytes to differentiation of luteal cells.
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Clinical Medicine, Tainan, Taiwan.
authors.forename: Hsien-An
authors.initials: HA
authors.lastname: Pan
authors.forename: Yue-Shan
authors.initials: YS
authors.lastname: Lin
authors.forename: Ko-Hung
authors.initials: KH
authors.lastname: Lee
authors.forename: Jin-Ru
authors.initials: JR
authors.lastname: Huang
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Lin
authors.forename: Pao-Lin
authors.initials: PL
authors.lastname: Kuo
mesh_terms: D000818:Animals; D000075364:Deleted in Azoospermia 1 Protein; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D010053:Ovary / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: C095668:DAZ1 protein, human; C101358:DAZL protein, human; D000075364:Deleted in Azoospermia 1 Protein; D016601:RNA-Binding Proteins