[Treatment with total hepatic vascular exclusion and reperfusion for the intestinal barrier in rats].
OBJECTIVE
To investigate the influence of treatment with total hepatic vascular exclusion and reperfusion on the intestinal barrier in rats.


METHODS
The total hepatic vascular exclusion and reperfusion model was built after the block of hepatic portal, suprahepatic and infraheptic vena cava for 20 minutes. Sixty Sprague-Dawley rats were divided randomly into 2 groups: sham operation group (Group A, n=30) and total hepatic vascular exclusion and reperfusion treatment group (Group B, n=30). Each group was subdivided randomly into 3 subgroups (n=10) according to different experiment time points as follows: at the end of the total hepatic vascular exclusion (T0), 4 reperfusion after total hepatic vascular exclusion (T1) and the 48 h survival. Portal vein blood gas was analysed at T0. At T0 and T1 the following items were detected: the level of portal vein blood D-lactate, tumor necrosis factor-alpha (TNF-alpha), the MDA concentration and pathologic morphology change of intestinal mucosa.


RESULTS
Compared with Group A, the PCO2 at T0 in Group B increased while pH, P02, and HCO3- decreased significantly (P < 0.05). The level of portal blood D-lactate, TNF-alpha and intestinal mucosa MDA at T0 and T1 was significantly higher (P < 0.05, or P < 0.01). The histologic damage in the intestinal mucosa was observed in Group B, and the rat survival in Group B was lower than that in Group A (P < 0.05).


CONCLUSIONS
The treatment with total hepatic vascular exclusion and reperfusion can damage the intestinal barrier in rats.
Cao
De-quan
DQ
Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China. caodequan989@hotmail.com
Chen
Yan-ping
YP
Li
Yong-guo
YG
Chang
Ye-tian
YT
D000818:Animals; D018988:Bacterial Translocation; D005260:Female; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology*; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology*; D008099:Liver / Q000098:blood supply*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology*