Long chain fatty acyl-CoA synthetase 5 expression is induced by insulin and glucose: involvement of sterol regulatory element-binding protein-1c.
In a screen for sterol regulatory element-binding protein (SREBP)-1c target genes in the liver, we identified long chain fatty acyl-CoA synthetase 5 (ACS-5). Hepatic ACS-5 mRNA is poorly expressed during fasting and diabetes and strongly induced by carbohydrate refeeding and insulin treatment. In cultured hepatocytes, insulin and a high glucose concentration induce ACS-5 mRNA. Adenoviral overexpression of a nuclear form of SREBP-1c in liver of diabetic mice or in cultured hepatocytes mimics the effect of insulin to induce ACS-5. By contrast, a dominant negative form of SREBP-1c abolishes the effect of insulin on ACS-5 expression. The dietary and SREBP-1c-mediated insulin regulation of ACS-5 expression indicate that ACS-5 is involved in the anabolic fate of fatty acids.
Achouri
Y
Y
Inserm Unit 671, Université Paris 6, Centre de Recherches Biomédicales des Cordeliers, 15, rue de l'Ecole de Médecine, 75270 Paris cedex 6, France.
Hegarty
B D
BD
Allanic
D
D
Bécard
D
D
Hainault
I
I
Ferré
P
P
Foufelle
F
F
D000818:Animals; D003066:Coenzyme A Ligases / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D004435:Eating; D004790:Enzyme Induction; D005215:Fasting; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology; D024101:Mitochondrial Proteins; D023421:Models, Animal; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D051780:Sterol Regulatory Element Binding Protein 1 / Q000502:physiology*
D005227:Fatty Acids; D007328:Insulin; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D003066:Coenzyme A Ligases; C508147:Acsl5 protein, rat; D005947:Glucose