title: Primary structure, recombinant expression, and molecular characterization of Phl p 4, a major allergen of timothy grass (Phleum pratense).
abstract: Grass pollen allergy is one of the most important allergic diseases world-wide. Several meadow grasses, like timothy grass and rye grass, contribute to allergic sensitizations, but also allergens from extensively cultivated cereals, especially rye, make a profound contribution. The group 4 allergens are well known as important major allergens of grasses. We have cloned for the first time group 4 sequences from Phleum pratense, Lolium perenne, Secale cereale, Triticum aestivum, and Hordeum vulgare, and investigated the IgE-reactivity of recombinant Phl p 4 as a candidate for allergy diagnostic and therapeutic applications.
authors.affiliation: Allergopharma Joachim Ganzer KG, Research and Development, Reinbek, Germany. andreas.nandy@allergopharma.de
authors.forename: Andreas
authors.initials: A
authors.lastname: Nandy
authors.forename: Arnd
authors.initials: A
authors.lastname: Petersen
authors.forename: Martin
authors.initials: M
authors.lastname: Wald
authors.forename: Roland
authors.initials: R
authors.lastname: Suck
authors.forename: Helga
authors.initials: H
authors.lastname: Kahlert
authors.forename: Bernhard
authors.initials: B
authors.lastname: Weber
authors.forename: Wolf-Meinhard
authors.initials: WM
authors.lastname: Becker
authors.forename: Oliver
authors.initials: O
authors.lastname: Cromwell
authors.forename: Helmut
authors.initials: H
authors.lastname: Fiebig
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D008969:Molecular Sequence Data; D031784:Phleum / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D000485:Allergens; C100980:Phl p 4 protein, Phleum pratense; D010940:Plant Proteins; D011994:Recombinant Proteins