[Biological stabilities and identification of a highly metastatic subpopulation from a human osteosarcoma cell line].
OBJECTIVE
To investigate the biological stabilities and the identification of a human highly metastatic osteosarcoma subpopulation.


METHODS
Tumor pieces of the cell were inoculated into nude mice orthotopically. Lung node metastatic foci were obtained by culturing, the procedure was repeated for 3 cycles. Changes were observed in intra-tibial tumor growth, pulmonary metastatic rate in vivo, morphological characteristics, expressions of BMP, vimentin, actin, NEC and karyotype analyses of every passage in vitro.


RESULTS
The intra-tibial tumor growth rates were 100%, the proliferation abilities were stable, and the pulmonary metastatic rates were higher than 80%. The expressions of BMP, vimentin, actin were positive, but the expression of NSE was negative. Its morphological characteristics the antigen-positive substances and karyotype were similar to those observed in human osteosarcoma.


CONCLUSIONS
SOSP-M1 with very stable biological characteristics is a good model for the study of human osteosarcoma.
Pan
Z
Z
Orthopaedic Institute of People's Liberation Army, 89th Hospital, Weifang 261021, China.
Fan
Q
Q
Wang
C
C
D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000473:pathology*