Detection of specific sequences in RNA using differential adsorption of single-stranded oligonucleotides on gold nanoparticles.
We have used the disparity in adsorption rates for single- and double-stranded RNA on ionically coated gold nanoparticles suspended in a colloid to design a rapid sequence identification assay. Unlabeled target RNA and a probe sequence are mixed prior to exposure to the gold nanoparticles to enable efficient hybridization. We have designed assays based on either color changes or fluorescence that are sensitive to a few picomoles of target. Single-base mutations on RNA sequences can be detected even in complex oligonucleotide mixtures. The assay requires less than 10 min so that RNA degradation problems are avoided.
Li
Huixiang
H
Department of Chemistry, University of Rochester, Rochester, New York 14627, USA.
Rothberg
Lewis
L
D000327:Adsorption; D001483:Base Sequence; D003124:Colorimetry; D006046:Gold / Q000737:chemistry*; D009154:Mutation / Q000235:genetics; D053758:Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012996:Solutions
D009841:Oligonucleotides; D012996:Solutions; D012313:RNA; D006046:Gold