[Research strategies towards a holistic characterization of rheumatoid arthritis--a systems biology approach].
Genome-wide screening methods used in functional genomics (genome, transcriptome, proteome and metabolom analysis) have increasingly been conducted in integrative research platforms to enable a comprehensive holistic characterization of multifactorial polygenic diseases. First results of this research strategy demonstrate that extended data sets are compiled whose quality is ensured by the application of standard operating procedures (SOPs) and the integration of specific laboratory information management systems (LIMS). Experimental data derived from this technology and methodology platform are obtained by applying standardized sampling procedures followed by comprehensive experimental validation and bioinformatic comparisons with the world knowledge publicly available. This research strategy should finally lead to a holistic understanding of the pathogenesis presented in rheumatoid arthritis by identifying disease-associated regulatory networks (pathways) and assigning them to cell populations involved in the disease mechanisms. In addition, it has to be investigated to what extent genetic as well as epigenetic factors direct disease initiation and progression in potential conjunction with environmental impacts (infections, smoking, etc.).
Thiel
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Deutsches Rheumaforschungszentrum, Berlin, Germany.
Thiesen
H-J
HJ
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000503:physiopathology* / Q000628:therapy; D016207:Cytokines / Q000235:genetics* / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology*; D023281:Genomics / Q000379:methods* / Q000639:trends; D006801:Humans; D012106:Research / Q000639:trends; D012107:Research Design; D049490:Systems Biology / Q000379:methods* / Q000639:trends
D016207:Cytokines