[Role of human leukocyte antigen-G small interference RNA in a choriocarcinoma cell line].
OBJECTIVE
To study the role of human leukocyte antigen-G (HLA-G) in the carcinogenesis and development of choriocarcinoma.


METHODS
We designed and synthesized a double strand small interference RNA (siRNA) of HLA-G, then transfected it into a HLA-G overexpressed choriocarcinoma cell line, JEG-3 by lipofectine; HLA-G mRNA level was detected by real time RT-PCR; HLA-G protein level was detected by western blot. The living cells of JEG-3 were counted under microscope after transfection by siRNA.


RESULTS
Double strand siRNA of HLA-G effectively downregulated the mRNA level and the protein level of HLA-G. The mRNA levels by Ct value at different concentration (1.0, 2.5, 5.0 microg/L) were 20.67 +/- 0.02, 21.37 +/- 0.03, 21.43 +/- 0.02, respectively. They were significantly different compared with the control groups which were 20.33 +/- 0.01, 20.37 +/- 0.02, 20.40 +/- 0.03, respectively (P < 0.05). The protein levels were down regulated in different concentration compared with the control groups (0.42 +/- 0.03 vs 1.69 +/- 0.23, 0.37 +/- 0.02 vs 1.62 +/- 0.31, 0.12 +/- 0.04 vs 1.36 +/- 0.22; P < 0.01). Through downregulation of HLA-G expression, the growth of choriocarcinoma cells was inhibited.


CONCLUSIONS
Small interference RNA of HLA-G can effectively down regulate HLA-G gene in mRNA and protein levels and inhibit tumor growth in choriocarcinoma. It shows that HLA-G plays an important role in the development of choriocarcinoma.
Chen
Chun-ling
CL
Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 100034, China.
Liao
Qin-ping
QP
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002822:Choriocarcinoma / Q000235:genetics* / Q000473:pathology; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D011247:Pregnancy; D012313:RNA / Q000032:analysis*; D034622:RNA Interference / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology*; D014162:Transfection
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D034741:RNA, Small Interfering; D012313:RNA