Susceptibility of different cell lines to infection with bovine respiratory syncytial virus.
The growth of bovine respiratory syncytial virus (BRSV) was evaluated in six different cell lines. Chicken embryo related cells (CER), a chicken embryo fibroblast/baby hamster kidney hybrid and bovine CRIB cells (a bovine viral diarrhea virus-resistant clone of MDBK cells) showed to be the most appropriate for virus multiplication. Both cells provided infectious virus titres of up to 10(5.5) 50% tissue culture infective doses per 100 microl (TCID(50)/100 microl). One-step growth curves revealed no significant differences in the growth of BRSV in these two cell lines. Furthermore, they proved to be susceptible to infection with three different BRSV strains. It was concluded that both CER and CRIB cells may be used for laboratory multiplication of BRSV with optimal results.
Spilki
Fernando Rosado
FR
Laboratório de Virologia Animal, Depto. de Microbiologia e Imunologia, Instituto de Biologia, UNICAMP, Brazil.
de Almeida
Renata Servan
RS
Campalans
Jacqueline
J
Arns
Clarice Weis
CW
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D002522:Chlorocebus aethiops; D006224:Cricetinae; D018112:Respiratory Syncytial Virus, Bovine / Q000254:growth & development*; D014709:Vero Cells; D014776:Virus Cultivation