Neuronal expression of keratinocyte-associated transmembrane protein-4, KCT-4, in mouse brain and its up-regulation by neurite outgrowth of Neuro-2a cells.
One group of proteins that regulates neurite outgrowth and maintains neuronal networks is the immunoglobulin superfamily (IgSF). We previously identified a new member of the IgSF, keratinocyte-associated transmembrane protein-4 (KCT-4), by the signal sequence-trap method from primary cultured human keratinocytes. The KCT-4 mRNA has been found to be highly expressed in the adult human brain, although it is also distributed in various tissues. In the present study, to gain insight into the role of KCT-4 in the nervous system, we examined the expression profile and localization of KCT-4 mRNA in mouse brain. We also evaluated changes in KCT-4 mRNA expression in the differentiation of the neuroblastoma cell line Neuro-2a as the in vitro model of neurite outgrowth. KCT-4 mRNA was detected broadly in various regions of the adult mouse brain by RT-PCR. In situ hybridization revealed that it was expressed highly selectively by neurons but not by glial cells. Moreover, expression of KCT-4 mRNA was induced by neurite outgrowth of Neuro-2a. These data suggest that KCT-4 participates in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
Yamada
Osamu
O
Department of Veterinary Clinical Pathology, Nippon Veterinary and Animal Science University, Musashino-shi, Tokyo 180-8602, Japan.
Tamura
Kyoichi
K
Yagihara
Hiroko
H
Isotani
Mayu
M
Washizu
Tsukimi
T
Bonkobara
Makoto
M
D000818:Animals; D015703:Antigens, CD; D015152:Blotting, Northern / Q000379:methods; D001921:Brain / Q000166:cytology*; D045744:Cell Line, Tumor; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D008565:Membrane Proteins; D051379:Mice; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D009447:Neuroblastoma / Q000503:physiopathology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D009418:S100 Proteins / Q000378:metabolism; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
D015703:Antigens, CD; D005904:Glial Fibrillary Acidic Protein; C440028:IGSF8 protein, human; D007136:Immunoglobulins; D008565:Membrane Proteins; D012333:RNA, Messenger; D009418:S100 Proteins; C489950:S100a3 protein, mouse; D014212:Tretinoin; D010751:Phosphopyruvate Hydratase