title: Therapeutic effect of Fe2O3 nanoparticles combined with magnetic fluid hyperthermia on cultured liver cancer cells and xenograft liver cancers.
abstract: The therapeutic effect of Fe2O3 nanoparticles combined with magnetic fluid hyperthermia (MFH) on human hepatocarcinoma SMMC-7721 cells in vitro and xenograft liver cancer in nude mice is studied. We examined growth and apoptosis of SMMC-7721 cells treated with MFH containing Fe2O3 nanoparticles at various concentrations (2, 4, 6, and 8 g/liter) for 30-60 min by using MTT, flow cytometry (FCM), and transmission electron microscopy (TEM). We also observed weight and volume inhibitory rates of the tumors of SMMC-7721-bearing nude mice by using animal experiments. The results showed that Fe2O3 nanoparticles combined with MFH could significantly inhibit the proliferation and increase the ratio of apoptosis of SMMC-7721 cells, and the effect was dose-dependent. The inhibitory rate was 26.5%, 33.53%, 54.4%, and 81.2%, respectively, and the apoptosis rate was 30.26%, 38.65%, 50.28%, and 69.33%, respectively. Animal experiments showed that tumors became small. The weight inhibitory ratio was 42.10%, 66.34%, 78.5%, and 91.46%, and the volume inhibitory ratio was 58.77%, 80.44%, 93.40%, and 98.30%, respectively. Compared with the control and experimental groups, each group had statistically significant difference (p < 0.05). So, Fe2O3 nanoparticles combined with MFH could inhibit the proliferation and induce apoptosis of SMMC-7721 cells and also has a significant inhibitory effect on the weight and volume of xenograft liver cancer. However, the mechanism remains to be further investigated.
authors.affiliation: Department of Pathology and Pathophysiology, Institute of Molecular Pathology, School of Basic Medical Sciences, Southeast University, Nanjing 210096, Jiangsu, P R. China.
authors.forename: Shiyan
authors.initials: S
authors.lastname: Yan
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Anwei
authors.initials: A
authors.lastname: Ding
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Wang
authors.forename: Baoling
authors.initials: B
authors.lastname: Xing
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003131:Combined Modality Therapy; D005290:Ferric Compounds / Q000627:therapeutic use*; D006801:Humans; D006979:Hyperthermia, Induced; D008113:Liver Neoplasms / Q000473:pathology / Q000628:therapy*; D008280:Magnetics / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008854:Microscopy, Electron; D049329:Nanostructures; D014183:Transplantation, Heterologous
chemical_list: D005290:Ferric Compounds; C001215:ferrite; C000499:ferric oxide