title: Liver transplantation using liver grafts preserved under high pressure.
abstract: To extend organ preservation time, we attempted to establish a unique method of maintaining a preservation solution in a stable unfrozen state below its freezing point by pressurizing the solution. Livers removed from Lewis rats (RT1l) were stored in UW solution pressurized at the prescribed pressure. After the termination of preservation, orthotopic liver transplantation was performed. Experiment 1: Liver grafts were pressurized up to 30, 40, 50, and 70 MPa and preserved at 0 degrees C for 60 min. Experiment 2: Liver grafts were compressed at a rate of 1.32 or 0.04 MPa/s to 35 MPa and preserved for 60 min at 0 degrees C. Experiments 3 and 4: Liver grafts were pressurized up to 5, 10, 20, and 30 MPa and preserved at -2 degrees C (Exp. 3), -3 degrees C or -4 degrees C (Exp. 4) for 5 h. All rats transplanted with livers pressurized up to 30 MPa (Exp. 1), all rats in the 5 MPa and control groups at -2 degrees C (Exp. 3), and all rats in the 5 MPa group at -3 degrees C (Exp. 4) survived for 2 weeks. In light microscopy, diffuse hemorrhage and vacuolar degeneration of hepatocytes were observed in a pressure-dependent manner. Liver grafts preserved under pressurized, subzero nonfrozen condition have sufficient function to sustain the life of rats after orthotopic transplantation.
authors.affiliation: Department of Surgery, Kitasato University School of Medicine, Sagamihara, Japan. tueno@partners.org
authors.forename: Takuya
authors.initials: T
authors.lastname: Ueno
authors.forename: Takashi
authors.initials: T
authors.lastname: Omura
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Harumi
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Akira
authors.initials: A
authors.lastname: Kakita
authors.forename: Shohei
authors.initials: S
authors.lastname: Yamashina
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods; D006085:Graft Survival; D008099:Liver; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D008853:Microscopy; D009926:Organ Preservation / Q000379:methods*; D011312:Pressure; D051381:Rats