Identification of Anopheles (Nyssorhynchus) albitarsis complex species (Diptera: Culicidae) using rDNA internal transcribed spacer 2-based polymerase chain reaction primes.
Anopheles (Nyssorhynchus) marajoara is a proven primary vector of malaria parasites in Northeast Brazil, and An. deaneorum is a suspected vector in Western Brazil. Both are members of the morphologically similar Albitarsis Complex, which also includes An. albitarsis and an undescribed species, An. albitarsis "B". These four species were recognized and can be identified using random amplified polymorphic DNA (RAPD) markers, but various other methodologies also point to multiple species under the name An. albitarsis. We describe here a technique for identification of these species employing polymerase chain reaction (PCR) primers based on ribosomal DNA internal transcribed spacer 2 (rDNA ITS2) sequence. Since this method is based on known sequence it is simpler than the sometimes problematical RAPD-PCR. Primers were tested on samples previously identified using RAPD markers with complete correlation.
Li
Cong
C
Dept. of Entomology, Walter Reed Army Institute of Research, Silver Spring, MD, 20910-7500, US.
Wilkerson
Richard C
RC
D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D001118:Argentina; D001483:Base Sequence; D001938:Brazil; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D007303:Insect Vectors / Q000145:classification / Q000235:genetics*; D008288:Malaria / Q000635:transmission; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D014687:Venezuela
D021903:DNA, Ribosomal Spacer