hDAF porcine cardiac xenograft maintains cardiac output after orthotopic transplantation into baboon--a perioperative study.
BACKGROUND
Only limited data are available on the physiological functional compatibility of cardiac xenografts after orthotopic pig to baboon transplantation (oXHTx). Thus we investigated hemodynamic parameters including cardiac output (CO) before and after oXHTx.


METHODS
Orthotopic xenogeneic heart transplantation from nine hDAF transgeneic piglets to baboons was performed. We used femoral arterial thermodilution for the invasive assessment of CO and stroke volume.


RESULTS
Baseline CO of the baboons after induction of anesthesia was 1.36 (1.0-1.9) l/min. 30 to 60 min after termination of the cardiopulmonary bypass, CO of the cardiac xenograft was significantly increased to 1.72 (1.3-2.1) l/min (P < 0.01). The stroke volumes of the baboon heart before transplantation and the cardiac xenograft was comparable [14.9 (11-26) vs. 11.8 (10-23) ml]. Thus the higher CO was achieved by an increase in heart rate after oXHTx [75.0 (69-110) vs. 140.0 (77-180)/min; P < 0.01]. Despite the increased CO, oxygen delivery was reduced [256 (251-354) vs. 227 (172-477) ml/min; P < 0.01] due to the inevitable hemodilution during the cardiopulmonary bypass and the blood loss caused by the surgical procedures.


CONCLUSIONS
Our results demonstrate that in the early phase after orthotopic transplantation of hDAF pig hearts to baboons, cardiac function of the donor heart is maintained and exceeds baseline CO. However, in the early intraoperative phase this was only possible by using inotropic substances and vasopressors due to the inevitable blood loss and dilution by the priming of the bypass circuit.
Bauer
Andreas
A
Clinic for Anaesthesiology, Ludwig-Maximilians University Munich, Munich, Germany.
Baschnegger
Heiko
H
Abicht
Jan M
JM
Brandl
Ulrike
U
Brenner
Paolo
P
Thein
Eckart
E
Hammer
Claus
C
Reichart
Bruno
B
Peter
Klaus
K
Schmoeckel
Michael
M
Christ
Frank
F
D000818:Animals; D030801:Animals, Genetically Modified; D018958:CD55 Antigens / Q000235:genetics* / Q000378:metabolism*; D002302:Cardiac Output / Q000502:physiology*; D016027:Heart Transplantation; D006801:Humans; D010215:Papio; D013552:Swine; D019076:Transgenes / Q000235:genetics; D014183:Transplantation, Heterologous
D018958:CD55 Antigens