Determination of telmisartan in human plasma by liquid chromatography-tandem mass spectrometry.
A rapid, selective and sensitive method for the determination of the angiotensin II receptor antagonist, telmisartan, in human plasma has been developed. Telmisartan and the internal standard, diphenhydramine, were extracted from plasma using diethyl ether-dichloromethane (60:40, v/v), and separated on a Zorbax extend C(18) column using methanol-10mM ammonium acetate (85:15, v/v) adjusted to pH 4.5 after mixing with formic acid as mobile phase. Detection was carried out by multiple reaction monitoring on a Q-trap LC-MS/MS system with an ESI interface. The assay was linear over the range 0.5-600.0 ng/ml with a limit of quantitation of 0.5 ng/ml and a limit of detection of 0.05 ng/ml. Intra- and inter-day precision were <6.7% and <8.1%, respectively, and the accuracy was in the range 88.9-111.0%. The assay was applied to a pharmacokinetic study of telmisartan given as a single oral dose (80 mg) to healthy volunteers.
Li
Pengfei
P
Research Center for Drug Metabolism, College of Life Science, Jilin University, Jiefang Road 123, Changchun 130023, China.
Wang
Yingwu
Y
Wang
Yan
Y
Tang
Yunbiao
Y
Fawcett
J Paul
JP
Cui
Yimin
Y
Gu
Jingkai
J
D001562:Benzimidazoles / Q000097:blood*; D001565:Benzoates / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D000077333:Telmisartan
D001562:Benzimidazoles; D001565:Benzoates; D000077333:Telmisartan