title: DNA microarray analysis of gene expression in eutopic endometrium from patients with deep endometriosis using laser capture microdissection.
abstract: OBJECTIVE
  To investigate differentially expressed genes in epithelial and stromal cells of eutopic endometrium from patients with deep endometriosis and women with normal pelvic cavities using laser capture microdissection and complementary DNA microarrays.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Patients with deep endometriosis and fertile women who underwent laparoscopic tubal ligation or reversal of tubal sterilization.
  
  
  METHODS
  Endometrial tissue biopsies during the late proliferative phase and early, mid-, and late secretory phases.
  
  
  METHODS
  Genes that were regulated with a change greater than threefold were selected as differentially expressed genes. Validation was performed with real-time reverse transcriptase-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Microarray analysis identified up-regulation during the late secretory phase (patients with endometriosis vs. controls) of several genes in two important signaling pathways: RAS/RAF/MAPK and PI3K. This included the genes RON, SOS, 14-3-3 protein eta, and uPAR in epithelial cells and KSR and PI3K p85 regulatory subunit alpha in stromal cells; real-time RT-PCR analysis validated up-regulation of all six genes.
  
  
  CONCLUSIONS
  The RAS/RAF/MAPK and PI3K pathways may be involved in initial development of endometriosis.
authors.affiliation: Department of Gynecology, Hŏtel-Dieu, Polyclinique, Centre Hospitalier Universitaire, Clermont-Ferrand, France. sachikoma@aol.com
authors.forename: Sachiko
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: Michel
authors.initials: M
authors.lastname: Canis
authors.forename: Catherine
authors.initials: C
authors.lastname: Vaurs-Barrière
authors.forename: Odile
authors.initials: O
authors.lastname: Boespflug-Tanguy
authors.forename: Bernard
authors.initials: B
authors.lastname: Dastugue
authors.forename: Gérard
authors.initials: G
authors.lastname: Mage
mesh_terms: D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000378:metabolism / Q000473:pathology*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011446:Prospective Studies; D018709:Statistics, Nonparametric