Radiation responses of perfused tracheal tissue.
We are using a novel perfusion system to examine the effects of radiation on a model respiratory tissue. Tracheas taken from young adult male Fischer 344 rats are embedded in a growth factor-enriched agarose matrix that is mounted in a special apparatus designed to allow growth medium to periodically wash the epithelial surface of the lumen. A comparison of the microarray expression profiles of freshly harvested tracheas and tracheas maintained in perfusion culture for 24 h shows no significant difference except for an increase in expression of a few metabolism- and surfactant-related genes. Perfusion culture samples exposed to 4 Gy of X rays show a lower than expected increase in expression for some cell cycle- and repair-related genes.
Ford
J R
JR
Department of Nuclear Engineering, Texas A&M University, College Station, Texas 77843-3133, USA. ford@ne.tamu.edu
Maslowski
A J
AJ
Redd
R A
RA
Braby
L A
LA
D000818:Animals; D015870:Gene Expression / Q000528:radiation effects; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010477:Perfusion; D051381:Rats; D011916:Rats, Inbred F344; D014132:Trachea / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*