title: Isolation of a novel flavanone 6-glucoside from the flowers of Carthamus tinctorium (Honghua) by high-speed counter-current chromatography.
abstract: A novel flavanone glycoside, (2S)-4',5,6,7-tetrahydroxyflavavone 6-O-beta-D-glucopyranoside was isolated from the ethyl acetate extract of the flowers of Carthamus tinctorium by high-speed counter-current chromatography (HSCCC). Using an optimized two-phase solvent system composed of ethyl acetate-methanol-water (5:1:5, v/v), target compound (52 mg) with purity of 98.0% was obtained from 2.0 g of sample by HSCCC in seven times run. The structure of the target compound was elucidated by means of spectroscopic methods including IR, MS, 1D and 2D NMR techniques.
authors.affiliation: School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, China.
authors.forename: Mingbo
authors.initials: M
authors.lastname: Zhao
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Pengfei
authors.initials: P
authors.lastname: Tu
mesh_terms: D031178:Carthamus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D044950:Flavanones / Q000302:isolation & purification*; D035264:Flowers / Q000737:chemistry*; D006027:Glycosides / Q000302:isolation & purification*
chemical_list: C504929:(2S)-4',5,6,7-tetrahydroxyflavavone 6-O-beta-D-glucopyranoside; D044950:Flavanones; D006027:Glycosides