title: Detection of ABCA7-positive cells in salivary glands from patients with Sjögren's syndrome.
abstract: ABCA7 is a member of the subfamily A of adenosine triphosphate-binding cassette (ABC) proteins, and highly homologous to ABCA1, which mediates the release of cellular cholesterol and phospholipid to form high-density lipoprotein. ABCA1 and ABCA7 contain two large extracellular domains, ECD1 and 2, which are thought to be important for their functions. Interestingly, part of ECD1 of ABCA7 is deposited as an autoantigen of Sjögren's syndrome. To determine the relationship between ABCA7 and Sjögren's syndrome, an immunohistochemical study was conducted with salivary gland biopsy samples from patients with Sjögren's syndrome. ECD1 of human ABCA7 (amino acids 45-549) was expressed in Escherichia coli as a protein fused with glutathione-S-transferase and a monoclonal antibody, KM3095, was generated. KM3095-immunoreactive cells were observed in salivary glands from 10 of 18 patients with Sjögren's syndrome. Immunostaining of serial sections with the plasma cell marker NCL-PC indicated that most of the plasma cells infiltrating salivary glands of patients with Sjögren's syndrome were KM3095-immunoreactive. Although the pathological or biological significance is not clear, it will be intriguing to further examine the relationship between ABCA7 and Sjögren's syndrome.
authors.affiliation: Center for Anatomical Studies, Graduate School of Medicine, Kyoto University, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Toda
authors.forename: Ryo
authors.initials: R
authors.lastname: Aoki
authors.forename: Yuika
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Yuya
authors.initials: Y
authors.lastname: Azuma
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kioka
authors.forename: Michinori
authors.initials: M
authors.lastname: Matsuo
authors.forename: Maya
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Shiro
authors.initials: S
authors.lastname: Mori
authors.forename: Manabu
authors.initials: M
authors.lastname: Fukumoto
authors.forename: Kazumitsu
authors.initials: K
authors.lastname: Ueda
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000276:immunology / Q000378:metabolism*; D000595:Amino Acid Sequence; D001324:Autoantigens / Q000276:immunology; D015415:Biomarkers / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000196:embryology; D008969:Molecular Sequence Data; D012469:Salivary Glands / Q000378:metabolism*; D012859:Sjogren's Syndrome / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D014162:Transfection
chemical_list: C411895:ABCA7 protein, human; D018528:ATP-Binding Cassette Transporters; D001324:Autoantigens; D015415:Biomarkers