title: Effects of three resin monomers on the cellular glutathione concentration of cultured human gingival fibroblasts.
abstract: OBJECTIVE
  Oral and systemic cells are permanently exposed to various types of xenobiotics, such as dental restorative materials, which may subsequently cause adverse effects. Objective of the present investigation was to analyze the effects of three important resin monomers on the glutathione metabolism of human gingival fibroblasts after an incubation period of 4h.
  
  
  METHODS
  Cells were exposed to various concentrations of 2-hydroxyethyl methacrylate (HEMA; 0.1-10 mM), triethylene-glycol dimethacrylate (TEGDMA; 0.05-2.5 mM), and urethane dimethacrylate (UDMA; 0.005-0.25 mM). Subsequently, cellular glutathione (GSH) concentrations were determined after a treatment period of 4h using the monobromobimane assay. Data were statistically evaluated using Tukey ANOVA with p<0.05.
  
  
  RESULTS
  GSH depletion was dependent on the type of the resin monomer: UDMA>TEGDMA>HEMA. The concentrations for a 50%-reduction of cellular GSH varied between 0.1 mM (0.05 mM) (UDMA), 0.33 mM (0.09 mM) (TEGDMA), and 1.6 mM (0.8 mM) (HEMA). Simultaneously, no decrease of cell numbers was found at any tested concentration.
  
  
  CONCLUSIONS
  These data indicate that the investigated resins may cause cell damage due to depletion of intracellular GSH level even at low concentrations within a short period of time. The decrease of GSH is an early reaction, which is triggered prior to other cytotoxic alterations.
authors.affiliation: Department of Conservative Dentistry and Periodontology, Medical University Hannover, D-30625 Hannover, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Volk
authors.forename: J
authors.initials: J
authors.lastname: Engelmann
authors.forename: G
authors.initials: G
authors.lastname: Leyhausen
authors.forename: W
authors.initials: W
authors.lastname: Geurtsen
mesh_terms: D001643:Bridged Bicyclo Compounds; D002452:Cell Count; D002478:Cells, Cultured; D003188:Composite Resins / Q000494:pharmacology*; D003764:Dental Materials / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005456:Fluorescent Dyes; D005881:Gingiva / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000032:analysis / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011109:Polymethacrylic Acids / Q000494:pharmacology*; D011140:Polyurethanes / Q000494:pharmacology*; D019279:Resin Cements / Q000494:pharmacology*; D013439:Sulfhydryl Reagents
chemical_list: D001643:Bridged Bicyclo Compounds; D003188:Composite Resins; D003764:Dental Materials; D005456:Fluorescent Dyes; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011140:Polyurethanes; D019279:Resin Cements; D013439:Sulfhydryl Reagents; C029824:urethane dimethacrylate luting resin; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate; D005978:Glutathione; C028381:monobromobimane