Alteration of collapsin response mediator protein-2 expression in focal ischemic rat brain.
Collapsin response mediator protein-2 is highly expressed in the adult brain and it has been speculated to play roles in nervous system diseases. Studies determined for the first time whether collapsin response mediator protein-2 expression is altered in brain ischemia. We observed ischemia-modulated expression of different-sized collapsin response mediator protein-2. As the ischemic duration increased, the expression level of a known major 62 kDa collapsin response mediator protein-2 was decreased, whereas the expression of a newly-detected 58 kDa collapsin response mediator protein-2 was clearly increased in middle carotid artery-occluded rat brain tissues. Analysis of the two collapsin response mediator protein-2 bands revealed that the novel 58 kDa collapsin response mediator protein-2 observed in middle carotid artery-occluded rat brain tissues was a cleavage form; the predicted cleavage site is located at the carboxy-terminal of the collapsin response mediator protein-2. These data suggest that collapsin response mediator protein-2 is an important candidate controlling ischemic stroke.
Chung
Myung-Ah
MA
Research Institute of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, Korea.
Lee
Ji-Eun
JE
Lee
Jeong-Young
JY
Ko
Moon-Jeong
MJ
Lee
Seung-Taek
ST
Kim
Hwa-Jung
HJ
D000595:Amino Acid Sequence; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D002339:Carotid Arteries / Q000502:physiology; D004396:Coloring Agents; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D020768:Middle Cerebral Artery / Q000502:physiology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013778:Tetrazolium Salts; D015854:Up-Regulation / Q000502:physiology
D004396:Coloring Agents; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D013778:Tetrazolium Salts; C095114:collapsin response mediator protein-2; C009591:triphenyltetrazolium