title: Inhibitory effects of Houttuynia cordata water extracts on anaphylactic reaction and mast cell activation.
abstract: The present study was investigated the effect of Houttuynia cordata THUNB water extract (HCWE) on mast cell-mediated anaphylactic reactions. The mast cell-mediated anaphylactic reaction is involved in many allergic diseases such as asthma and allergic rhinitis. HCWE has been used as a traditional medicine in Korea and is known to have an antioxidant and anti-cancer activities. However, its specific effect of mast cell-mediated anaphylactic reactions is still unknown. We examined whether HCWE could inhibit compound 48/80-induced systemic anaphylaxis, IgE-mediated passive cutaneous anaphylaxis (PCA), and mast cell activation. The oral administration of HCWE inhibited compound 48/80-induced systemic anaphylaxis in mice. HCWE also inhibited the local allergic reaction, PCA, activated by anti-dinitrophenyl (DNP) IgE antibody in rats. HCWE reduced the compound 48/80-induced mast cell degranulation and colchicine-induced deformation of rat peritoneal mast cells (RPMC). Moreover, HCWE dose-dependently inhibited histamine release and calcium uptake of RPMC induced by compound 48/80 or anti-DNP IgE. HCWE increased the level of intracellular cAMP and inhibited significantly the compound 48/80-induced cAMP reduction in RPMC. These results suggest that HCWE may be beneficial in the treatment of mast cell-mediated anaphylactic responses.
authors.affiliation: Department of Anatomy, Yanbian University Medical School; Yanji City, 133000, China.
authors.forename: Guang Zhao
authors.initials: GZ
authors.lastname: Li
authors.forename: Ok Hee
authors.initials: OH
authors.lastname: Chai
authors.forename: Moo Sam
authors.initials: MS
authors.lastname: Lee
authors.forename: Eui-Hyeog
authors.initials: EH
authors.lastname: Han
authors.forename: Hyoung Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Song
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006636:Histamine Release; D052859:Houttuynia; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; C442781:Houttuynia cordata extract; D003189:p-Methoxy-N-methylphenethylamine; D000242:Cyclic AMP; D002118:Calcium