A study of cytokines released from fibroblasts in cultured dermal substitute.
Allogeneic cultured dermal substitute (CDS) was prepared by culturing fibroblasts on a two-layered spongy matrix of hyaluronic acid (HA) and atelo-collagen (Col). CDS can be cryopreserved and transported to other hospitals in a frozen state. The present study was designed to analyze amounts of cytokines released from fibroblasts in fresh or cryopreserved CDS. The culture medium used in preparing CDS over a cultivation period of 1 week (fresh CDS culture medium sample) contained vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), platelet derived growth factor (PDGF)-AA, transforming growth factor (TGF)-beta1, keratinocyte growth factor (KGF), interleukin (IL)-6 and IL-8. After thawing of cryopreserved CDS, the CDS was re-cultured in medium for 1 week. The culture medium used in re-culturing CDS for 1 week (cryopreserved CDS culture medium sample) contained VEGF, bFGF, and HGF in the same concentration as before freezing, and TGF-beta1 and IL-8 at half the concentration before freezing. Levels of PDGF-AA, KGF, and IL-6 were significantly less than before freezing. This finding suggests that the cryopreserved CDS retains its ability to release VEGF, bFGF, and HGF that are essential for wound healing.
Kubo
Kentaro
K
Regenerative Tissue Engineering, Graduate School of Medical Sciences, Kitasato University, Sagamihara, Kanagawa, Japan.
Kuroyanagi
Yoshimitsu
Y
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D015925:Cryopreservation; D016207:Cytokines / Q000378:metabolism*; D020405:Dermis / Q000254:growth & development / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods
D000276:Adjuvants, Immunologic; D016207:Cytokines; C059821:atelocollagen; D006820:Hyaluronic Acid; D003094:Collagen