title: Protective effect of carnosine during nitrosative stress in astroglial cell cultures.
abstract: Formation of nitric oxide by astrocytes has been suggested to contribute, via impairment of mitochondrial function, to the neurodegenerative process. Mitochondria under oxidative stress are thought to play a key role in various neurodegenerative disorders; therefore protection by antioxidants against oxidative stress to mitochondria may prove to be beneficial in delaying the onset or progression of these diseases. Carnosine has been recently proposed to act as antioxidant in vivo. In the present study, we demonstrate its neuroprotective effect in astrocytes exposed to LPS- and INFgamma-induced nitrosative stress. Carnosine protected against nitric oxide-induced impairment of mitochondrial function. This effect was associated with decreased formation of oxidatively modified proteins and with decreased up-regulation oxidative stress-responsive genes, such as Hsp32, Hsp70 and mt-SOD. Our results sustain the possibility that carnosine might have anti-ageing effects to brain cells under pathophysiological conditions leading to degenerative damage, such as aging and neurodegenerative disorders.
authors.affiliation: Department of Chemical Sciences, University of Catania, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Calabrese
authors.forename: C
authors.initials: C
authors.lastname: Colombrita
authors.forename: E
authors.initials: E
authors.lastname: Guagliano
authors.forename: M
authors.initials: M
authors.lastname: Sapienza
authors.forename: A
authors.initials: A
authors.lastname: Ravagna
authors.forename: V
authors.initials: V
authors.lastname: Cardile
authors.forename: G
authors.initials: G
authors.lastname: Scapagnini
authors.forename: A M
authors.initials: AM
authors.lastname: Santoro
authors.forename: A
authors.initials: A
authors.lastname: Mangiameli
authors.forename: D A
authors.initials: DA
authors.lastname: Butterfield
authors.forename: A M
authors.initials: AM
authors.lastname: Giuffrida Stella
authors.forename: E
authors.initials: E
authors.lastname: Rizzarelli
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D002336:Carnosine / Q000494:pharmacology*; D002478:Cells, Cultured; D017931:DNA Primers; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism*; D015538:Nitrosation; D018384:Oxidative Stress / Q000235:genetics; D051381:Rats; D015854:Up-Regulation
chemical_list: D017931:DNA Primers; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D009569:Nitric Oxide; D007371:Interferon-gamma; D002336:Carnosine