Primary structure, recombinant expression, and molecular characterization of Phl p 4, a major allergen of timothy grass (Phleum pratense).
Grass pollen allergy is one of the most important allergic diseases world-wide. Several meadow grasses, like timothy grass and rye grass, contribute to allergic sensitizations, but also allergens from extensively cultivated cereals, especially rye, make a profound contribution. The group 4 allergens are well known as important major allergens of grasses. We have cloned for the first time group 4 sequences from Phleum pratense, Lolium perenne, Secale cereale, Triticum aestivum, and Hordeum vulgare, and investigated the IgE-reactivity of recombinant Phl p 4 as a candidate for allergy diagnostic and therapeutic applications.
Nandy
Andreas
A
Allergopharma Joachim Ganzer KG, Research and Development, Reinbek, Germany. andreas.nandy@allergopharma.de
Petersen
Arnd
A
Wald
Martin
M
Suck
Roland
R
Kahlert
Helga
H
Weber
Bernhard
B
Becker
Wolf-Meinhard
WM
Cromwell
Oliver
O
Fiebig
Helmut
H
D000485:Allergens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D008969:Molecular Sequence Data; D031784:Phleum / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
D000485:Allergens; C100980:Phl p 4 protein, Phleum pratense; D010940:Plant Proteins; D011994:Recombinant Proteins