title: Regulation of hair regrowth in alopecic site of IFN-gamma-/- mice by macrophages infiltrating into allograft in IFN-gamma+/+ mice.
abstract: We previously demonstrated that around 6 weeks of age, most of the interferon-gamma (IFN-gamma)-/- C57BL/6 mice began to lose morphogenesis-derived hairs in their dorsal and occipital areas and that hair regrowth in the alopecic site was induced by intraperitoneal (i.p.) injection of IFN-gamma and allogeneic Meth A cells. Here, we explored the IFN-gamma mRNA expression in the cells infiltrating into allograft in IFN-gamma(+)/(+) mice by RT-PCR and adoptively transferred specific antigen-minus infiltrates into IFN-gamma-/- mice to assess the hair regrowth inducibility. IFN- gamma mRNA was expressed in the infiltrates on days 3-8 after allografting, with a peak on day 3 or 4, and CD4(+) and F4/80(+) cells were the major producers of IFN-gamma. All infiltrates on day 3 induced hair regrowth, whereas those on days 0-2 or 4-8 were ineffective or partially effective, respectively. The removal of F4/80(+) macrophages from all infiltrates failed to induce hair regrowth, whereas the removal of Ly-6C(+) macrophages rather accelerated the hair regrowth. These results showed that F4/80(+), Ly-6C(+), and CD4(+) and F4/80(+) cells were stimulatory, inhibitory, and IFN-gamma-producing cells, respectively, in the regulation of hair regrowth.
authors.affiliation: Department of Physiology, Osaka Medical College, Takatsuki, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Okada
authors.forename: Junko
authors.initials: J
authors.lastname: Tashiro-Yamaji
authors.forename: Takeshi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Hayahito
authors.initials: H
authors.lastname: Nomi
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Satoko
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Kouichi
authors.initials: K
authors.lastname: Ueda
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kubota
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Yoshida
mesh_terms: D019264:Adoptive Transfer; D000505:Alopecia / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D000950:Antigens, Ly / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D002465:Cell Movement; D006197:Hair / Q000254:growth & development*; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D008264:Macrophages / Q000737:chemistry / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009368:Neoplasm Transplantation; D014184:Transplantation, Homologous
chemical_list: D000943:Antigens, Differentiation; D000950:Antigens, Ly; D015704:CD4 Antigens; C494192:Ly-6C antigen, mouse; C036763:monocyte-macrophage differentiation antigen; D007371:Interferon-gamma