title: Changes of dehydrogenases activity in soils polluted with diesel fuel.
abstract: Activity of the enzymes can give information on the kind and duration of the effects of pollutants on the metabolic activity of soil. Results of studies on the dehydrogenase activity in soils experimentally contaminated with diesel fuel, with and without modification used in investigation over biodegradation process (including fertilization, stirring and inoculation) were presented in paper. Studies with two rates of pollution were carried out for soils of various organic matter content and different soil particle size grading. Dehydrogenase activity was determined with TTC (2,3,5-triphenyltetrazolium chloride) as a substrate. Increase of dehydrogenase activity was found in the soils that were treated with diesel fuel. Regardless of pollution rate, usually higher activity was recorded in sandy soil. Variance analysis confirmed the highly significant influence of applied modifications on increase of the enzymatic activity in relation to not modified object.
authors.affiliation: Department of Microbiology and Environmental Biotechnology, University of Agriculture, Slowackiego 17, 71-434 Szczecin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Hawrot
authors.forename: Andrzej
authors.initials: A
authors.lastname: Nowak
authors.forename: Dariusz
authors.initials: D
authors.lastname: Kłódka
mesh_terms: D001673:Biodegradation, Environmental; D005742:Gasoline; D010088:Oxidoreductases / Q000378:metabolism*; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000378:metabolism
chemical_list: D005742:Gasoline; D012987:Soil; D012989:Soil Pollutants; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium; D010088:Oxidoreductases