title: Differential DNA methylation of gene promoters in small B-cell lymphomas.
abstract: Improved care of patients with small B-cell lymphomas (SBCLs) is likely to result from the ongoing discovery of molecular markers that better define these malignant neoplasms. We identified multiple gene loci whose DNA methylation patterns differed between 3 types of SBCL: B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma, mantle cell lymphoma, and grades I and II follicular lymphoma. This analysis was performed using an oligonucleotide microarray that allowed determination of the DNA methylation status of 156 loci in 38 genes. Combined bisulfite restriction analysis and methylation-specific polymerase chain reaction were used to validate the differential methylation of 6 of these genes. By using non-Hodgkin lymphoma cell lines as models, these genes were examined further for methylation and gene expression relationships. This study illustrates nonrandom epigenetic alterations in SBCLs that seem to preferentially involve lymphomas of germinal center derivation.
authors.affiliation: Department of Pathology and Anatomical Sciences, University of Missouri School of Medicine, Columbia 65203, USA.
authors.forename: Juyuan
authors.initials: J
authors.lastname: Guo
authors.forename: Matthias
authors.initials: M
authors.lastname: Burger
authors.forename: Inko
authors.initials: I
authors.lastname: Nimmrich
authors.forename: Sabine
authors.initials: S
authors.lastname: Maier
authors.forename: Evelyne
authors.initials: E
authors.lastname: Becker
authors.forename: Buelent
authors.initials: B
authors.lastname: Genc
authors.forename: Dieter
authors.initials: D
authors.lastname: Duff
authors.forename: Farahnaz
authors.initials: F
authors.lastname: Rahmatpanah
authors.forename: Rebecca
authors.initials: R
authors.lastname: Chitma-Matsiga
authors.forename: Huidong
authors.initials: H
authors.lastname: Shi
authors.forename: Kurt
authors.initials: K
authors.lastname: Berlin
authors.forename: Tim H-M
authors.initials: TH
authors.lastname: Huang
authors.forename: Charles W
authors.initials: CW
authors.lastname: Caldwell
mesh_terms: D019175:DNA Methylation; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic