title: Febrile nonhaemolytic transfusion reaction caused by antibodies against human platelet antigen 5a.
abstract: Anti-human platelet antigens (HPA) alloantibodies are seldom involved in febrile nonhaemolytic reactions (FNHTRs). We describe a case in which anti-HPA-5a alloantibodies are related to an FNHTR. We studied the specificity of the alloantibodies by flow cytometry, ELISA and MACE. Typing of donors and the patient was performed by sequence-specific polymerase chain reaction. The alloantibodies were found reactive with HPA-5a antigens. The patient was HPA-5b/b, whereas the donor of the platelet apheresis involved in the FNHTR was HPA-5a/a. Despite the low frequency of anti-HPA-5a antibodies, they might be responsible for FNHTR.
authors.affiliation: Immunohematology and Transfusion Medicine Service, Azienda Ospedaliera S.Orsola-Malpighi, Bologna, Italy.
authors.forename: P L
authors.initials: PL
authors.lastname: Tazzari
authors.forename: A
authors.initials: A
authors.lastname: Bontadini
authors.forename: C
authors.initials: C
authors.lastname: Zamagni
authors.forename: F
authors.initials: F
authors.lastname: Ricci
authors.forename: F
authors.initials: F
authors.lastname: Fruet
authors.forename: S
authors.initials: S
authors.lastname: Iannelli
authors.forename: R
authors.initials: R
authors.lastname: Conte
mesh_terms: D000328:Adult; D016824:Antigens, Human Platelet / Q000276:immunology; D001782:Blood Donors; D005260:Female; D006461:Hemolysis / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000235:genetics / Q000276:immunology; D017713:Platelet Transfusion / Q000009:adverse effects / Q000379:methods; D013921:Thrombocytopenia / Q000628:therapy
chemical_list: C071092:5a alloantigen, human; D016824:Antigens, Human Platelet; D007518:Isoantibodies