title: Down-regulation of cardiac bradykinin B2 receptors and eNOs mRNA in rats with remnant kidneys.
abstract: The changes in the expression of cardiac bradykinin B2 receptors (BKB2Rs) and endogenous nitrix oxide synthase (eNOs) mRNA were studied in rats with remnant kidneys. Thirty-two rats were divided into sham-operated and experimental groups randomly (n = 16 in each group). The remnant kidney model was established by 2-stage 5/6 nephrectomy. Blood pressure and serum Cr were measured before operation and 15, 30, 60, 120 days after 5/6 nephrectomy. Eight animals in each group were killed at the first month and 4th month after the operation. The expression of BKB2Rs and eNOs mRNAs was detected by using RT-real time PCR from isolated left ventricle, and their correlation was also analyzed. The results showed that blood pressure and serum Cr were increased significantly 15 days after 5/6 nephrectomy (both P<0.01), and the hypertension and azomia existed constantly till 120 days but had no significant fluctuation. Cardiac BKB2Rs and eNOs mRNA was declined time-dependently (both P<0.05). And there was a close positive correlation between cardiac BKB2Rs and eNOs mRNA (r= 0.82, P< 0.01). It was suggested that a significant chronic renal failure can be produced at least 15 days after 5/6 nephrotomy and can sustain more than 4 months. The expression of BKB2Rs and eNOs was down-regulated time-dependently in this model, and there was a significant correlation between them.
authors.affiliation: Geriatric Department, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Huaibin
authors.initials: H
authors.lastname: Wan
authors.forename: Ling
authors.initials: L
authors.lastname: Tu
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Liu
authors.forename: Juanjaun
authors.initials: J
authors.lastname: Deng
mesh_terms: D000818:Animals; D015536:Down-Regulation; D007676:Kidney Failure, Chronic / Q000209:etiology / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009392:Nephrectomy; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D043782:Receptor, Bradykinin B2 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D043782:Receptor, Bradykinin B2; D052250:Nitric Oxide Synthase Type III