Carbohydrate-deficient isoforms of transferrin (%CDT) and sialic acid (SA) in iron-deficiency anemia.
This study has investigated the serum levels of carbohydrate-deficient isoforms of transferrin (CDT) and sialic acid (SA) in iron-deficiency anemia (IDA). Blood samples were collected from 60 women with IDA and from 20 healthy controls. CDT was estimated by anion-exchange chromatography on minicolumns followed by photometric detection of transferrin and was expressed as a percentage of total transferrin (%CDT). SA was measured by an enzymatic method. There was no difference in the mean level of %CDT between patients with IDA (2.26%) and control patients (2.05%). SA increased significantly from control level 0.61 to 0.69 g/l in anemic patients. We concluded that elevated concentration of total transferrin in IDA did not change the relative value of low sialylated isoforms (%CDT) and the increase of total SA level in the sera of anemic patients is not related to the increase of total transferrin.
Cylwik
B
B
Department of Biochemical Diagnostics, Medical University, Bialystok, Poland.
Chrostek
L
L
Szmitkowski
M
M
Kĺoczko
J
J
Koput
A
A
D000328:Adult; D018798:Anemia, Iron-Deficiency / Q000097:blood*; D016022:Case-Control Studies; D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D008875:Middle Aged; D019158:N-Acetylneuraminic Acid / Q000097:blood*; D020033:Protein Isoforms; D014168:Transferrin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry
D020033:Protein Isoforms; D014168:Transferrin; C056759:carbohydrate-deficient transferrin; D019158:N-Acetylneuraminic Acid