title: Determination and validation of a simple high-performance liquid chromatographic method for simultaneous assay of iprodione and vinclozolin in human urine.
abstract: A method based on solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) was developed for the simultaneous determination of 3-(3,5-diclorophenyl)-5-ethenyl-5-methyl-2,4-oxazolidinedione (vinclozolin) and 3-(3,5-diclorophenyl)-N-(1-methylethyl)-2,4-dioxo-1-imidazolidinecarboxamide (iprodione) in human urine. Urine samples containing vinclozolin and iprodione were collected by solid phase extraction using C(18) cartridges. The chromatographic separation was achieved on a Spherisorb ODS2 (250 mm x 4.6 mm, 5 microm) column with an isocratic mobile phase of acetonitrile-water (60:40, v/v). Detection was UV absorbance at 220 nm. The calibration graphs were linear from 30 to 1000 ng/mL for the two fungicides. Intra- and inter-day R.S.D. did not exceed 2.9%. The quantitation limit was 50 ng/mL for vinclozolin and 30 ng/mL for iprodione, respectively.
authors.affiliation: Dipartimento di Scienze del Farmaco, Facoltà di Farmacia, Università degli Studi "G. D'Annunzio" Chieti, Via dei Vestini, 66100 Chieti, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Carlucci
authors.forename: Dorina Di
authors.initials: DD
authors.lastname: Pasquale
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Ruggieri
authors.forename: Pietro
authors.initials: P
authors.lastname: Mazzeo
mesh_terms: D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006827:Hydantoins / Q000652:urine*; D010080:Oxazoles / Q000652:urine*; D015203:Reproducibility of Results; D015262:Xenobiotics / Q000032:analysis / Q000737:chemistry
chemical_list: D006827:Hydantoins; D010080:Oxazoles; D015262:Xenobiotics; D000620:Aminoimidazole Carboxamide; C025643:vinclozolin; C033148:iprodione