title: Genetic engineering of terpenoid metabolism attracts bodyguards to Arabidopsis.
abstract: Herbivore-damaged plants release complex mixtures of volatiles that attract natural enemies of the herbivore. To study the relevance of individual components of these mixtures for predator attraction, we manipulated herbivory-induced volatiles through genetic engineering. Metabolic engineering of terpenoids, which dominate the composition of many induced plant volatile bouquets, holds particular promise. By switching the subcellular localization of the introduced sesquiterpene synthase to the mitochondria, we obtained transgenic Arabidopsis thaliana plants emitting two new isoprenoids. These altered plants attracted carnivorous predatory mites (Phytoseiulus persimilis) that aid the plants' defense mechanisms.
authors.affiliation: Laboratory of Entomology, Wageningen University, Post Office Box 8031, 6700 EH Wageningen, Netherlands.
authors.forename: Iris F
authors.initials: IF
authors.lastname: Kappers
authors.forename: Asaph
authors.initials: A
authors.lastname: Aharoni
authors.forename: Teun W J M
authors.initials: TW
authors.lastname: van Herpen
authors.forename: Ludo L P
authors.initials: LL
authors.lastname: Luckerhoff
authors.forename: Marcel
authors.initials: M
authors.lastname: Dicke
authors.forename: Harro J
authors.initials: HJ
authors.lastname: Bouwmeester
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D031985:Fragaria / Q000201:enzymology / Q000235:genetics; D018390:Gene Targeting; D017343:Genes, Plant; D005818:Genetic Engineering; D006836:Hydro-Lyases / Q000235:genetics* / Q000378:metabolism; D008925:Mites / Q000502:physiology; D008928:Mitochondria / Q000201:enzymology; D010572:Pest Control, Biological; D010675:Pheromones; D030821:Plants, Genetically Modified; D011235:Predatory Behavior; D012717:Sesquiterpenes / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: C411254:4,8-dimethyl-1,3,7-nonatriene; D010675:Pheromones; D012717:Sesquiterpenes; D013729:Terpenes; C010411:insect attractants; D006836:Hydro-Lyases; C092055:S-linalool synthase; C037055:nerolidol