title: Regulation of noncapacitative calcium entry by arachidonic acid and nitric oxide in endothelial cells.
abstract: Several peptides, including vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), activate the release of arachidonic acid (AA) and nitric oxide (NO) in endothelial cells (ECs). Both messengers are involved in EC proliferation and vascular permeability and control calcium homeostasis in different ways. Interestingly, it has been recently suggested that NO acts as a downstream mediator of AA-induced calcium entry in smooth muscle cells and isolated mouse parotid cells. In this paper, we have investigated the complex relationships that link intracellular calcium, AA, and NO in cultured endothelial cells. Using different experimental approaches, mainly simultaneous Ca2+ and NO fluorimetric confocal imaging, we provide evidence for a complex pathway leading to noncapacitative calcium entry (NCCE) in bovine aortic endothelial cells (BAECs). In particular, AA is able to induce NCCE through two different pathways: one dependent on eNOS recruitment and NO release, the other NO-independent. Finally, we show that NO increase is involved in the control of BAEC proliferation.
authors.affiliation: Department of Animal and Human Biology, University of Torino, Torino, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Mottola
authors.forename: Susanna
authors.initials: S
authors.lastname: Antoniotti
authors.forename: Davide
authors.initials: D
authors.lastname: Lovisolo
authors.forename: Luca
authors.initials: L
authors.lastname: Munaron
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001011:Aorta / Q000166:cytology; D016718:Arachidonic Acid / Q000378:metabolism / Q000494:pharmacology*; D001692:Biological Transport; D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002417:Cattle; D049109:Cell Proliferation; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000378:metabolism; D004594:Electrophysiology; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology*; D005456:Fluorescent Dyes / Q000494:pharmacology; D006706:Homeostasis; D007249:Inflammation; D018613:Microscopy, Confocal; D008954:Models, Biological; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D018919:Neovascularization, Physiologic; D009569:Nitric Oxide / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D018408:Patch-Clamp Techniques; D010477:Perfusion; D010539:Permeability; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D015220:Calcium Channels; D005456:Fluorescent Dyes; D016718:Arachidonic Acid; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III; D006152:Cyclic GMP; D002118:Calcium; D019331:NG-Nitroarginine Methyl Ester