title: Lipid peroxidation induces cholesterol domain formation in model membranes.
abstract: Numerous reports have established that lipid peroxidation contributes to cell injury by altering the basic physical properties and structural organization of membrane components. Oxidative modification of polyunsaturated phospholipids has been shown, in particular, to alter the intermolecular packing, thermodynamic, and phase parameters of the membrane bilayer. In this study, the effects of oxidative stress on membrane phospholipid and sterol organization were measured using small angle x-ray diffraction approaches. Model membranes enriched in dilinoleoylphosphatidylcholine were prepared at various concentrations of cholesterol and subjected to lipid peroxidation at physiologic conditions. At cholesterol-to-phospholipid mole ratios (C/P) as low as 0.4, lipid peroxidation induced the formation of discrete, membrane-restricted cholesterol domains having a unit cell periodicity or d-space value of 34 A. The formation of cholesterol domains correlated directly with lipid hydroperoxide levels and was inhibited by treatment with vitamin E. In the absence of oxidative stress, similar cholesterol domains were observed only at C/P ratios of 1.0 or higher. In addition to changes in sterol organization, lipid peroxidation also caused reproducible changes in overall membrane structure, including a 10 A reduction in the width of the surrounding, sterol-poor membrane bilayer. These data provided direct evidence that lipid peroxidation alters the essential organization and structure of membrane lipids in a manner that may contribute to changes in membrane function during aging and oxidative stress-related disorders.
authors.affiliation: Elucida Research, Beverly, Massachusetts 01915-0091, USA. rjacob@elucidaresearch.com
authors.forename: Robert F
authors.initials: RF
authors.lastname: Jacob
authors.forename: R Preston
authors.initials: RP
authors.lastname: Mason
mesh_terms: D002784:Cholesterol / Q000737:chemistry* / Q000378:metabolism*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D008567:Membranes, Artificial; D008954:Models, Biological; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology; D014961:X-Ray Diffraction
chemical_list: D008563:Membrane Lipids; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D014810:Vitamin E; C002448:1,2-linoleoylphosphatidylcholine; D002784:Cholesterol