Attachment of adeno-associated virus type 3H to fibroblast growth factor receptor 1.
Heparan sulfate proteoglycan is thought to act as primary receptor for adeno-associated virus type 2 (AAV-2). Reported coreceptors include alphaVbeta5 integrin, fibroblast growth factor receptor 1 (FGFR-1), and hepatocyte growth factor (c-Met). The interaction of AAV type 3 (AAV-3) with possible cell membrane receptors is incompletely defined. In this study, using assays detecting competition with viral infection, virus binding inhibition assays and dot blotting, we show attachment of AAV-3 strain H to heparin, heparan sulfate, and FGFR-1. These findings provide new information on the possible receptor array used by AAV-3.
Blackburn
S D
SD
Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah 84602, USA.
Steadman
R A
RA
Johnson
F B
FB
D001665:Binding Sites; D002460:Cell Line; D000229:Dependovirus / Q000145:classification / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006493:Heparin / Q000378:metabolism; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*
D006493:Heparin; D006497:Heparan Sulfate; D051496:Receptor, Fibroblast Growth Factor, Type 1; D011991:Receptors, Virus; C496348:FGFR1 protein, human