Heparin-released extracellular superoxide dismutase is reduced in patients with coronary artery atherosclerosis.
OBJECTIVE
We studied whether the amount of heparin-released extracellular superoxide dismutase (EC-SOD), which is an antioxidative enzyme, is associated with coronary artery disease (CAD).


RESULTS
EC-SOD was measured in plasma at basal and at post-heparin injection in 315 patients. Heparin-released EC-SOD was calculated as the difference between the two values. After exclusion of a mutant EC-SOD group (n = 27:8.6%), 288 patients were divided into three groups by angiographic findings; those with normal coronary (the normal group; n = 63), those with atherosclerosis without significant stenosis (the mild atherosclerosis group; n = 36), and those with significant stenosis (the atherosclerosis group; n = 189). Although the basal values were similar among the three groups, heparin-released EC-SOD levels were significantly lower in the atherosclerosis group (131.0 +/- 42.8 ng/ml, p = 0.0003) than in the normal group (156.9 +/- 66.2 ng/ml). Moreover, logistic analysis revealed that heparin-released EC-SOD independently contributed to CAD. The coronary score showed a significant correlation with heparin-released EC-SOD. As for factors affecting the level of heparin-released EC-SOD, the level of high-density lipoprotein cholesterol and age showed a positive correlation.


CONCLUSIONS
The results suggest that heparin-released EC-SOD is significantly reduced in CAD and that the tissue-bound location of this enzyme might be important for antioxidative function.
Tasaki
Hiromi
H
Second Department of Internal medicine, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan. h-tasaki@med.ueoh-u.ac.jp
Yamashita
Kazuhito
K
Tsutsui
Masato
M
Kamezaki
Fumihiko
F
Kubara
Takahiro
T
Tanaka
Seiya
S
Sasaguri
Yasuyuki
Y
Adachi
Tetsuo
T
Nakashima
Yasuhide
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000792:Angiography; D000975:Antioxidants / Q000378:metabolism; D050197:Atherosclerosis; D015992:Body Mass Index; D003324:Coronary Artery Disease / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008075:Lipoproteins, HDL / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D012307:Risk Factors; D013482:Superoxide Dismutase / Q000378:metabolism*
D000975:Antioxidants; D008055:Lipids; D008075:Lipoproteins, HDL; D006493:Heparin; D013482:Superoxide Dismutase