title: Structure of a non-camelized human M12-V(H) domain at 1.5A resolution.
abstract: A non-camelized human V(H) domain has been crystallized through limited in vitro proteolysis of scFvM12 antibody fragment. The protease addition results in the complete degradation of the M12-V(L) domain, linker, and purification tags. The structure solved up to 1.5A resolution having good stereochemistry with a R(cryst) factor of 15.8% and R(free) factor of 19.7%. Dihedral angle values comparison of the first and the second complementarity-determining region (CDR) of M12-V(H) domain with an average values show a significant deviation; therefore, M12-V(H) domain structure indicates either the existence of a new canonical subclass or a link among the subclasses of canonical main-chain conformation in V(H)3 family. The presence of uncommon hydrogen bond between Ser-H50 and Tyr-H97 has pulling effect on CDR-H3 loop. The interface area buried by CDR-H3 loop indicates the partial coverage of the hydrophobic V(L)-V(H) interface. The isolated M12-V(H) domain was found soluble up to 0.35 mM. This result would be helpful in structure based designing of an isolated human single domain antibody fragments for biotechnological and pharmaceutical applications such as cancer.
authors.affiliation: Institute for Molecular Biotechnology VII, Aachen University, Worringerwegl, 52074 Aachen, Germany. gaur@molbiotech.rwth-aachen.de
authors.forename: Rajneesh Kumar
authors.initials: RK
authors.lastname: Gaur
mesh_terms: D000595:Amino Acid Sequence; D022801:Complementarity Determining Regions / Q000737:chemistry / Q000235:genetics; D018360:Crystallography, X-Ray; D006801:Humans; D006860:Hydrogen Bonding; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry* / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary
chemical_list: D022801:Complementarity Determining Regions; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region