[Construction of infectious Japanese encephalitis virus clone based on the cDNA template of the attenuated live vaccine production strain SA14-14-2].
OBJECTIVE
To construct infectious Japanese encephalitis virus (JEV) based on the in vitro-ligated cDNA template of the vaccine strain SA14-14-2, and identify the virus.


METHODS
Full-length genomic cDNA of JEV SA14-14-2 strain was ligated and then RNA was transcribed in vitro, the infective virus was obtained by transfecting the RNA into Vero cells and identified.


RESULTS
The infective clone of JEV was constructed, the virulence was weaker than the wild virus.


CONCLUSIONS
It was possible to construct infectious clone from the production strain of live attenuated Japanese B encephalitis vaccine.
Zeng
Ming
M
National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
Jia
Li-li
LL
Yu
Yong-xin
YX
Dong
Guan-mu
GM
Liu
Wen-xue
WX
Wang
Zhi-wei
ZW
Li
De-fu
DF
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D004672:Encephalitis, Japanese / Q000473:pathology / Q000821:virology; D016679:Genome, Viral; D022321:Japanese Encephalitis Vaccines / Q000276:immunology; D051379:Mice; D012367:RNA, Viral / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000276:immunology; D014709:Vero Cells; D014774:Virulence
D018076:DNA, Complementary; D022321:Japanese Encephalitis Vaccines; D012367:RNA, Viral; D014613:Vaccines, Attenuated