Novel 13C direct detection experiments, including extension to the third dimension, to perform the complete assignment of proteins.
Carbon-13 direct detection NMR methods are feasible thanks to the improvements in probehead technology and to the development of new NMR experiments. We present here a complete set of experiments, based on C' direct detection, developed to perform protein complete assignment of backbone and side-chains (except for aromatic rings). This strategy offers alternative solutions for demanding situations (paramagnetic and/or large molecules) and can be useful in general in conjunction with conventional experiments.
Bermel
Wolfgang
W
Bruker BioSpin GmbH, Rheinstetten, Germany.
Bertini
Ivano
I
Felli
Isabella C
IC
Kümmerle
Rainer
R
Pierattelli
Roberta
R
D002247:Carbon Isotopes; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D013482:Superoxide Dismutase / Q000737:chemistry*
D002247:Carbon Isotopes; D013482:Superoxide Dismutase