Multilayer membranes via layer-by-layer deposition of organic polymer protected Prussian blue nanoparticles and glucose oxidase for glucose biosensing.
Polyelectrolyte multilayers (PEMs) are now widely used for bioanalytical applications. In this work, a bilayer of poly(diallydimethylammonium chloride) (PDDA) and poly(sodium 4-styrenesulfonate) (PSS) is consecutively adsorbed on 3-mercapto-1-propanesulfonic acid modified Au electrode surfaces, forming stable, ultrathin multilayer films. Subsequently, Prussian blue nanoparticles protected by PDDA (denoted as P-PB) and negatively charged glucose oxidase (GOx) are consecutively adsorbed onto the PSS-terminated bilayer. The growth of each of the P-PB/GOx bilayers is followed quantitatively using UV-visible absorption spectroscopy and the electrochemical method. The P-PB nanoparticles can catalyze the electroreduction of hydrogen peroxide formed from enzymatic reaction at lower potential and inhibit the responses of interferents, such as ascorbic acid (AA) and uric acid (UA). Performance of the multilayer films can be tailored by controlling the number of bilayers. Under optimal conditions, a linear range of 0.10 to 11.0 mM and a detection limit of 10 microM were achieved. The glucose biosensor has good stability and reproducibility.
Zhao
Wei
W
The Key Lab of Analytical Chemistry for Life Science (Education Ministry of China), Department of Chemistry, Nanjing University, Nanjing 210093, P. R. China.
Xu
Jing-Juan
JJ
Shi
Chuan-Guo
CG
Chen
Hong-Yuan
HY
D015374:Biosensing Techniques; D004396:Coloring Agents; D004563:Electrochemistry / Q000379:methods; D005295:Ferrocyanides; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D053758:Nanoparticles
D004396:Coloring Agents; D005295:Ferrocyanides; D008567:Membranes, Artificial; D005949:Glucose Oxidase; D005947:Glucose; C000170:ferric ferrocyanide