title: Asymmetric dimethylarginine, homocysteine and renal function--is there a relation?
abstract: The adverse effect of hyperhomocysteinemia on the vascular wall can be partially explained by increasing plasma concentration of asymmetric dimethylarginine (ADMA), a potent inhibitor of nitric oxide synthase. The aim of the study was to compare ADMA and homocysteine levels in three groups of subjects: blood donors with normal homocysteine concentration (group A), patients with hyperhomocysteinemia and normal kidney function (group B) and hemodialysis patients who are known to be hyperhomocysteinemic (group C). Concentrations of homocysteine (enzymatic method), ADMA (enzyme-linked immunoassay) and creatinine (Jaffe method) in EDTA plasma were measured. Plasma ADMA levels were significantly higher in both groups with hyperhomocysteinemia (1.60+/-0.56 micromol/L in group B, 1.81+/-0.57 micromol/L in group C) when compared with those in blood donors (0.82+/-0.29 micromol/L, p<0.001 in both cases). Significant positive correlations were found between concentrations of ADMA and homocysteine (r=0.42, p<0.0001), ADMA and creatinine (r=0.39 p<0.001), homocysteine and creatinine (r=0.69, p<0.0001), age and homocysteine (r=0.47, p<0.001), age and ADMA (r=0.57, p<0.001) and age and creatinine (r=0.37, p<0.001). Increased ADMA concentrations in hyperhomocysteinemic patients were confirmed, but multiple linear regression analysis showed that this significant correlation is only apparent due the dependence of both parameters on age.
authors.affiliation: Institute of Clinical Biochemistry and Hematology, Charles University, Medical Faculty, Pilsen, Czech Republic. sirokar@fnplzen.cz
authors.forename: Romana
authors.initials: R
authors.lastname: Siroká
authors.forename: Ladislav
authors.initials: L
authors.lastname: Trefil
authors.forename: Daniel
authors.initials: D
authors.lastname: Rajdl
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Racek
authors.forename: Hana
authors.initials: H
authors.lastname: Rusnáková
authors.forename: Romana
authors.initials: R
authors.lastname: Cibulka
authors.forename: Jaromír
authors.initials: J
authors.lastname: Eiselt
authors.forename: Jan
authors.initials: J
authors.lastname: Filipovský
mesh_terms: D000328:Adult; D000367:Age Factors; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D020138:Hyperhomocysteinemia / Q000097:blood; D007668:Kidney / Q000502:physiology*; D007677:Kidney Function Tests; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis
chemical_list: D006710:Homocysteine; C018524:N,N-dimethylarginine; D001120:Arginine