Splenic macrophages and B cells mediate immunosuppression following abrupt withdrawal from morphine.
We have previously shown that abrupt withdrawal (AW) from morphine induces greater than 80% immunosuppression in murine spleen cells, as assessed by the capacity to mount an in vitro plaque-forming cell response to sheep red blood cells. Present studies about the mechanisms of immunosuppression following AW showed that addition of highly enriched (CD11b+) splenic macrophages (obtained by cell sorting or magnetic separation) from AW mice to cultures of normal, unfractionated spleen cells suppressed immune responses. Further, addition of highly enriched (CD19+) B cells (but not T cells) from AW mice to normal cells was also immunosuppressive. B cells from AW mice were also able to inhibit the proliferative response of normal spleen cells to concanavalin A but not to lipopolysaccharide. Overall, the data suggest that immunosuppression by AW spleen cells is a result of active suppression by macrophages and B cells.
Rahim
Rahil T
RT
Department of Microbiology and Immunology, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, PA 19140, USA.
Meissler
Joseph J
JJ
Adler
Martin W
MW
Eisenstein
Toby K
TK
D000208:Acute Disease; D000818:Animals; D018941:Antigens, CD19 / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D018845:CD11 Antigens / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D003208:Concanavalin A / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D007108:Immune Tolerance / Q000187:drug effects / Q000276:immunology*; D018189:Immunomagnetic Separation; D018836:Inflammation Mediators / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D009020:Morphine / Q000009:adverse effects; D009021:Morphine Dependence / Q000276:immunology* / Q000503:physiopathology; D009294:Narcotics / Q000009:adverse effects; D013154:Spleen / Q000276:immunology* / Q000503:physiopathology; D013375:Substance Withdrawal Syndrome / Q000276:immunology* / Q000503:physiopathology
D018941:Antigens, CD19; D018845:CD11 Antigens; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D009294:Narcotics; D003208:Concanavalin A; D009020:Morphine