[Studies on chemical constitutes of green alga Chaetomorpha basiretorsa and their bioactivity].
OBJECTIVE
To study the chemical constituents of Chaetomorpha basiretorsa and screen for bioactive leading compounds.


METHODS
Compounds were isolated by normal phase silica gel and Sephadex LH-20 gel column chromatography, reverse phase MPLC and reverse phase HPLC. Their structures were elucidated by spectroscopic methods including MS, IR and 1D, 2D NMR. Cytotoxicity of the compounds was screened by using standard MTT method. The purified compounds' inhibition against proliferation of dog vascular smooth muscle cells was also screened by MTT assay.


RESULTS
Five compounds were isolated from C. basiretorsa and their structures were identified as euphol (I), loloilide (II), 4-cumylphenol (III), zeaxanthin (IV) and lactucaxanthin (V).


CONCLUSIONS
All these compounds were obtained from this genus for the first time. Compound (III), 4-cumylphenol, was a new nature product. All compounds were inactive (IC50 > 10 microg x mL(-1)) in cytotoxicity screening. In inhibition against proliferation of dog vascular smooth muscle cells test, the cell survival ratio to compound I was (0.32 +/- 0.056)% which indicate its potential anti-atherosclerotic bioactivity.
Shi
Da-Yong
DY
Department of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Han
Li-Jun
LJ
Sun
Jie
J
Yang
Yong-Chun
YC
Shi
Jian-Gong
JG
Fan
Xiao
X
D000818:Animals; D045744:Cell Line, Tumor / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000460:Chlorophyta / Q000737:chemistry*; D004285:Dogs; D006801:Humans; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D024341:Xanthophylls / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D065146:Zeaxanthins
D010636:Phenols; D014315:Triterpenes; D024341:Xanthophylls; D065146:Zeaxanthins; D007810:Lanosterol; C516284:4-cumylphenol; C062557:euphol