[Determination of fumonisin biosynthesis gene and toxicity of Fusarium moniliforme strains isolated in China].
OBJECTIVE
To study the relationship between fumonisin biosynthesis gene and toxicity of Fusarium moniliforme isolated in China.


METHODS
The toxigenic gene of 29 Fusarium moniliforme isolated from different provinces and varied food samples were determined. Eighteen fum5-positive strains were selected for biosyhesizing fumonisin and determined by high performance liquid chromatography (HPLC).


RESULTS
Twenty-six isolates were identified as fum5 gene positive strains. And all of these strains produced FB(1), FB(2) and FB(3). The amount of FB(1), FB(2) and FB(3) was ranging from 0.41-140.20 mg/kg, 0.06-14.30 mg/kg to 0.30-58.00 mg/kg, except one strain produced a lower level of FB(1) only. It wight be the first report showing a high level fumonisin-producing strain isolated from the sesame sample and identified in the world. The amount of FB(1), FB(2) and FB(3) produced by the isolate was 128.84 mg/kg, 11.80 mg/kg and 14.88 mg/kg.


CONCLUSIONS
It should have a close relationship between fumonisins biosynthesis gene and toxicity of Fusarium moniliforme isolated in China. The study demonstrated that strain of Fusarium moniliforme might contaminate the sesame sample and produce a high level of fumonisins.
Liu
Xiu-mei
XM
National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wang
Xiao-ying
XY
Qiu
Mao-feng
MF
Li
Xiu-fang
XF
D002681:China; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D037341:Fumonisins / Q000032:analysis / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005670:Fusarium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D031685:Sesamum / Q000382:microbiology
D037341:Fumonisins; D005656:Fungal Proteins