title: Novel 13C direct detection experiments, including extension to the third dimension, to perform the complete assignment of proteins.
abstract: Carbon-13 direct detection NMR methods are feasible thanks to the improvements in probehead technology and to the development of new NMR experiments. We present here a complete set of experiments, based on C' direct detection, developed to perform protein complete assignment of backbone and side-chains (except for aromatic rings). This strategy offers alternative solutions for demanding situations (paramagnetic and/or large molecules) and can be useful in general in conjunction with conventional experiments.
authors.affiliation: Bruker BioSpin GmbH, Rheinstetten, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Bermel
authors.forename: Ivano
authors.initials: I
authors.lastname: Bertini
authors.forename: Isabella C
authors.initials: IC
authors.lastname: Felli
authors.forename: Rainer
authors.initials: R
authors.lastname: Kümmerle
authors.forename: Roberta
authors.initials: R
authors.lastname: Pierattelli
mesh_terms: D002247:Carbon Isotopes; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D013482:Superoxide Dismutase / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D013482:Superoxide Dismutase