Mutagenic activity of estradiol evaluated by an in vitro micronucleus assay. Short communication.
The objective of the present study was to evaluate possible genetic changes in cultured human lymphocytes treated with estradiol, using the cytokinesis block micronucleus assay. Eight experimental concentrations of estradiol were used (range from 10(-10) M to 0.7 x 10(-4) M). The obtained results indicate that estradiol exhibits aneugenic and/or clastogenic effects, expressed as increased frequency of micronucleated lymphocytes at two highest experimental concentrations used in this investigation. In addition to genotoxic effects, these concentrations decreased the cytokinesis block proliferation index (CBPI) and percentage of binucleated cells, indicating the cell cycle delay and possible cytotoxic effects. In conclusion, estradiol treatment might represent a human health risk, especially if overdosed or used for a prolonged period of time.
Djelić
N
N
Faculty of Veterinary Medicine, University of Belgrade, Bul. JNA 18, 11000 Belgrade, Serbia. n.djelic@ptt.yu
Spremo-Potparević
Biljana
B
Djelić
Dijana
D
D000328:Adult; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D009153:Mutagens / Q000494:pharmacology* / Q000633:toxicity
D009153:Mutagens; D004958:Estradiol