[Total hepatic ischemia and reperfusion under normal conditions and submitted to controlled hemorrhagic shock state: effects of neutrophil sequestration in kidney of rats].
OBJECTIVE
The evaluate the effects of total hepatic ischemia and reperfusion under normal conditions and submitted to controlled hemorrhagic shock state on the accumulation of neutrophils in the cortex renal of rats.


METHODS
In this experiment 32 male adult Wistar mice, were divided into four groups: Control Group (CG), submitted to the catheterizations and laparotomy, followed by a period 60 minutes of observation; Shock Group (SG): controlled of hemorrhagic shock (CHS) for 20 minutes (20'), mean arterial pressure (MAP) 40 mmHg, followed by reanimation and reperfusion for 60 minutes (RP60); Pringle Group (PG): Pringle's Maneuver (PM) for 15 minutes (15'), followed for RP60'; Shock-Pringle Group (SPG): CHS (MAP = 40 mmHg) for 20' followed by laparotomy and PM for 15', followed for RP60'. To characterize the poor tissular perfusion blood lactate and the base reserve were measured.


RESULTS
The means values neutrophils were: CG = 0.24 (+/-0.28); SG = 1.06 (+/-0.61); PG = 0.18 (+/-0.16); SPG = 0.24 (+/-0.19). The CG demonstrated significant neutrophils accumulation in the renal córtex, when compared between others groups.


CONCLUSIONS
In this experimental study showed that CHS for 20' followed for RP60 was principal factor by major sequestration of neutrophils in the renal cortex of rats.
Hirano
Elcio Shiyoiti
ES
Departamento de Cirurgia, Faculdade de Ciências Médicas, Universidade Estadual de Campinas (Unicamp). elciohirano@ig.com.br
Mantovani
Mario
M
Morandin
Rosana Celestina
RC
D000818:Animals; D007422:Intestines / Q000473:pathology; D007511:Ischemia / Q000473:pathology*; D007668:Kidney / Q000473:pathology*; D007672:Kidney Cortex / Q000473:pathology; D019344:Lactic Acid / Q000097:blood; D008099:Liver / Q000098:blood supply*; D008297:Male; D009504:Neutrophils / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion / Q000009:adverse effects*; D012771:Shock, Hemorrhagic / Q000473:pathology*
D019344:Lactic Acid