[The modulating of aloe polysaccharides on the cell cycle and cycle regulating protein expression in X-ray irradiated non-malignant cells].
OBJECTIVE
To study the modulating of Aloe Polysaccharides on the cell cycle and cycle regulating protein expression in X-ray irradiated non-malignant cells.


METHODS
The cell cycle was analyzed by flow cytometric analyzed. The levels of cell cycle regulating protein expression were tested by Western blot.


RESULTS
A distinct G2/M block happened in 293 and C. Liver cells after irradiation. The pre-treatment of AP in the concentration of 50 microg/ml caused an increasing G0/G1 phase population and decreasing G2/M phase population. Meanwhile, pre-treatment of AP could significantly decrease the high expression of p53 protein caused by irradiation and evidently enhance the expression of P21, Cyclin B1 and pRb protein. Pre-treatment of AP had no evident effect on p27,CDK4 and Cyclin D1 protein.


CONCLUSIONS
There is a radioprotective effect of AP on non-malignant cells. This effect is related to alleviating the cell cycle turbulence. The modulating of Aloe Polysaccharides on the cell cycle regulating protein expression in X-ray irradiated non-malignant cells contributes to its alleviating effect on the cell cycle turbulence.
Wang
Zongwei
Z
Guangzhou University of TCM School of Chinese Materia Medica, Guangzhou.
Huang
Zhaosheng
Z
Wu
Qingguang
Q
Zhou
Junmin
J
Zhu
Xiaofeng
X
Li
Qiaomei
Q
Liu
Zongchao
Z
D000504:Aloe / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D018797:Cell Cycle Proteins / Q000096:biosynthesis*; D002460:Cell Line; D019926:Cyclin B / Q000378:metabolism; D056744:Cyclin B1; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000166:cytology; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011832:Radiation Injuries / Q000517:prevention & control; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014965:X-Rays
C534699:CCNB1 protein, human; D018797:Cell Cycle Proteins; D019926:Cyclin B; D056744:Cyclin B1; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D011837:Radiation-Protective Agents; D016159:Tumor Suppressor Protein p53