[Synthesis of substituted 3H-indole quaternary ammonium salt and study on its interaction with bovine serum albumin by fluorescence].
A substituted 3H-indole quaternary ammonium molecule was designed and synthesized using hexamethylphosphoramide (HMPA) as a solvent. The products were purified and characterized by IR, 1H-NMR, MS and elemental analysis. The binding reaction of this compound with bovine serum albumin (BSA) in aqueous solution was studied using fluorescence. Their binding constant is Ka = 1.995 x 10(5) dm3 x mol(-1) and the binding site number is n = 1.12. It is confirmed that the combination is a single static quenching process.
Xu
Tong-kuan
TK
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Shen
Xing-hai
XH
Gao
Hong-cheng
HC
D000465:Algorithms; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D005453:Fluorescence; D006492:Hempa / Q000737:chemistry; D007211:Indoles / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D015394:Molecular Structure; D011485:Protein Binding; D000644:Quaternary Ammonium Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
D007211:Indoles; D000644:Quaternary Ammonium Compounds; D014867:Water; D012710:Serum Albumin, Bovine; D006492:Hempa