title: Expressions and clinical significances of angiopoietin-1, -2 and Tie2 in human gastric cancer.
abstract: The roles of angiopoietins in gastric cancer progression are still not fully understood. In this study the expressions of angiopoietin-1 (Ang-1), -2 (Ang-2) were compared by immunohistochemistry in 53 gastric cancer and 23 normal gastric mucosa samples. Results revealed that Ang-2 expression was significantly increased in gastric cancer tissues (74%) and was correlated with higher TNM stage, lymph node metastasis as well as distance metastasis. The expression of Ang-1 was also elevated in cancerous tissues (66%) and significantly associated with differentiation degree. In addition, Ang-2 as well as its receptor Tie2 expressions were higher in 12 pairs of gastric cancer tissue samples than those in corresponding adjacent samples by Western blot, while Ang-1 expression showed great heterogeneity. Furthermore, the expressions of Ang-1 and Ang-2 were almost positive in eight gastric cancer cell lines. Among them, AGS expressed both Ang-2 and a relatively moderate amount of Ang-2(443), a novel splice form of Ang-2, while others showed only Ang-2 mRNA expression.
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Disease, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Kaichun
authors.initials: K
authors.lastname: Wu
authors.forename: Dexin
authors.initials: D
authors.lastname: Zhang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Tang
authors.forename: Huahong
authors.initials: H
authors.lastname: Xie
authors.forename: Liu
authors.initials: L
authors.lastname: Hong
authors.forename: Yanglin
authors.initials: Y
authors.lastname: Pan
authors.forename: Mei
authors.initials: M
authors.lastname: Lan
authors.forename: Shengjuan
authors.initials: S
authors.lastname: Hu
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Ning
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D042683:Angiopoietin-1 / Q000235:genetics / Q000378:metabolism*; D042702:Angiopoietin-2 / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D042787:Receptor, TIE-2 / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology
chemical_list: D042683:Angiopoietin-1; D042702:Angiopoietin-2; D012333:RNA, Messenger; D042787:Receptor, TIE-2