title: Stochastic modeling of S. cerevisiae inactivation by supercritical CO2.
abstract: Pasteurization of S. cerevisiae in a simple substrate with supercritical CO(2) was performed at 36 degrees C on a laboratory multibatch apparatus of a total volume of 150 mL. The pressure values ranged from 100 to 300 bar. The results show a clear dependence between inactivation ratio and increase of pressure. A mathematical modeling of the process was exploited to fit the experimental evidences: inactivation curves were analyzed using a stochastic model based on the multihit model (1). The nonlinear survival curve shows a shoulder and a tail which represent the lag and the resistant phase, respectively. The meaning of the nonlinear relationship between inactivation ratio and time is also discussed; the effect of pressure on the values assumed by the parameters of the model proposed was investigated.
authors.affiliation: Department of Material Engineering and Industrial Technologies, University of Trento, via Mesiano 77, 38050 Trento, Italy. sara.spilimbergo@ing.unitn.it
authors.forename: S
authors.initials: S
authors.lastname: Spilimbergo
authors.forename: D
authors.initials: D
authors.lastname: Mantoan
mesh_terms: D002245:Carbon Dioxide / Q000008:administration & dosage*; D002470:Cell Survival / Q000187:drug effects*; D015169:Colony Count, Microbial / Q000379:methods*; D003198:Computer Simulation; D050296:Microbial Viability / Q000187:drug effects*; D008954:Models, Biological; D015233:Models, Statistical; D011312:Pressure; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D013242:Sterilization / Q000379:methods*; D013269:Stochastic Processes; D016019:Survival Analysis
chemical_list: D002245:Carbon Dioxide