Detection and characterization of Streptococcus thermophilus bacteriophages by use of the antireceptor gene sequence.
In the dairy industry, the characterization of Streptococcus thermophilus phage types is very important for the selection and use of efficient starter cultures. The aim of this study was to develop a characterization system useful in phage control programs in dairy plants. A comparative study of phages of different origins was initially performed based on their morphology, DNA restriction profiles, DNA homology, structural proteins, packaging mechanisms, and lifestyles and on the presence of a highly conserved DNA fragment of the replication module. However, these traditional criteria were of limited industrial value, mainly because there appeared to be no correlation between these variables and host ranges. We therefore developed a PCR method to amplify VR2, a variable region of the antireceptor gene, which allowed rapid detection of S. thermophilus phages and classification of these phages. This method has a significant advantage over other grouping criteria since our results suggest that there is a correlation between typing profiles and host ranges. This association could be valuable for the dairy industry by allowing a rational starter rotation system to be established and by helping in the selection of more suitable starter culture resistance mechanisms. The method described here is also a useful tool for phage detection, since specific PCR amplification was possible when phage-contaminated milk was used as a template (detection limit, 10(5) PFU ml(-1)).
Binetti
Ana G
AG
Instituto de Productos Lácteos de Asturias (CSIC), Apdo. de correos 85, 33300 Villaviciosa, Asturias, Spain.
Del Río
Beatriz
B
Martín
M Cruz
MC
Alvarez
Miguel A
MA
D000818:Animals; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis; D007218:Industrial Microbiology / Q000379:methods; D008854:Microscopy, Electron; D008892:Milk / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D017108:Streptococcus Phages / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D048249:Streptococcus thermophilus / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics*
D004279:DNA, Viral; D014764:Viral Proteins