Infinite average lifetime of an unstable bright state in the green fluorescent protein.
The time evolution of the fluorescence intensity emitted by well-defined ensembles of green fluorescent proteins has been studied by using a standard confocal microscope. In contrast with previous results obtained in single-molecule experiments, the photobleaching of the ensemble is well described by a model based on Lévy statistics. By assuming the presence of thermally activated barriers, this simple model allows us to obtain information about their height distribution.
Didier
P
P
Institut de Physique et Chimie des Matériaux de Strasbourg, UMR 7504 CNRS-ULP-ECPM, 23 rue du Loess, B.P. 43, F-67034 Strasbourg CX, France. pdidier@ipcms.u-strasbg.fr
Guidoni
L
L
Bardou
F
F
D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D018613:Microscopy, Confocal; D008956:Models, Chemical; D038761:Photobleaching; D013269:Stochastic Processes
D049452:Green Fluorescent Proteins