[Increase of mitochondrial DNA copies with low level of DNA repair in tissue cells of gamma-irradiated mice].
The damage and the change in the number of mitochondrial DNA (mtDNA) copies in brain and spleen tissues of gamma-irradiated mice were studied. The changes in the number of mitochondrial DNA (mtDNA) copies were assayed by the comparative analysis of the density values of long-extension PCR products of the mtDNA fragments (16 kb) and the cluster nuclear gene of beta-globin (8.7 kb). PCRs of mtDNA fragments and the nuclear gene of beta-globin were carried out simultaneously in one test-tube within total DNA. Our results showed that in brain and in spleen cells of mice exposed to gamma-radiation an increase in copy number (polyploidization) of mtDNA with regard to the nuclear gene beta-globin took place. The induction of polyploidization of mtDNA observed in cells of gamma-irradiated animals is regarded as the development of a compensatory reaction because of the energy deficiency due to the increased ATP consumption and structural alteration of genes controlling OXPHOS. The data enabled the assumption that because of the low efficiency of repair systems in mitochondria the induction of synthesis of new mtDNA copies on intact or little affected mtDNA templates may be the major mechanism for the retention of the mitochondrial genome which is constantly damaged by the endogenous ROS and is affected by ionizing radiation and/or other exogenous factors.
Antipova
V N
VN
Malakhova
L V
LV
Ushakova
T E
TE
Sirota
N P
NP
Fomenko
L A
LA
Bezlepkin
V G
VG
Gaziev
A I
AI
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D004260:DNA Repair; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D005720:Gamma Rays; D005914:Globins / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D013997:Time Factors
D004272:DNA, Mitochondrial; D005914:Globins