title: Immunogenicity and protective effect of a DNA construct encoding certain neutralizing epitopes of herpes simplex virus type-1 glycoprotein B.
abstract: Much attention is presently focused on the vaccination with certain epitopes of an antigen. To further study the ability of neutralizing epitopes mapped in the first 1515 nucleotides of glycoprotein B of herpes simplex virus type-1 (gB-1) to induce neutralizing antibodies, a DNA immunization approach was employed. Vaccination of mice with a plasmid expressing the neutralizing epitopes induced humoral immune responses, although the antibody titre was significantly lower than that of antibodies induced by the full-length gB-1 gene. Furthermore, the plasmid DNA could not protect the mice against HSV-1 lethal challenge, but could significantly prolong the survival time compared to mock-vaccinated group.
authors.affiliation: Department of Virology, School of Medical Sciences, Tarbiat Modarres University, Tehran, Iran. Bamdad_T@modares.ac.ir
authors.forename: T
authors.initials: T
authors.lastname: Bamdad
authors.forename: M H
authors.initials: MH
authors.lastname: Roostaee
authors.forename: M
authors.initials: M
authors.lastname: Sadeghizadeh
authors.forename: F
authors.initials: F
authors.lastname: Mahboudi
authors.forename: A
authors.initials: A
authors.lastname: Kazemnejad
authors.forename: H
authors.initials: H
authors.lastname: Soleimanjahi
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D002460:Cell Line; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D000939:Epitopes / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D006561:Herpes Simplex / Q000276:immunology / Q000517:prevention & control*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D019444:Vaccines, DNA / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
chemical_list: D000906:Antibodies; D004279:DNA, Viral; D000939:Epitopes; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus