title: Efficient strategy for the rapid backbone assignment of membrane proteins.
abstract: Investigations of membrane proteins pose one of the biggest current challenges in structural biology. Recent advances in protein production techniques based on cell-free transcription/translation methods have, however, opened new opportunities in this area. Here, we report an efficient protocol for the backbone assignment of membrane proteins as the first step of NMR-based structure determination.
authors.affiliation: Institute for Biophysical Chemistry, University of Frankfurt and Center for Biomolecular Magnetic Resonance, 60439 Frankfurt, Germany.
authors.forename: Nikola
authors.initials: N
authors.lastname: Trbovic
authors.forename: Christian
authors.initials: C
authors.lastname: Klammt
authors.forename: Alexander
authors.initials: A
authors.lastname: Koglin
authors.forename: Frank
authors.initials: F
authors.lastname: Löhr
authors.forename: Frank
authors.initials: F
authors.lastname: Bernhard
authors.forename: Volker
authors.initials: V
authors.lastname: Dötsch
mesh_terms: D018360:Crystallography, X-Ray; D008565:Membrane Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation
chemical_list: D008565:Membrane Proteins