title: Capillary high-performance liquid chromatography/electrospray ion trap time-of-flight mass spectrometry using a novel nanoflow gradient generator.
abstract: A type of high-performance liquid chromatography (HPLC) based on a novel nanoflow gradient generator (Asymptotic-Trace-10-Port-Valve (AT10PV) nanoGR generator) was developed and coupled with an electrospray ion trap time-of-flight mass spectrometer (ESI-IT-TOF MS). Stability of the nanoflow GR HPLC system was tested at flow rates of 20 and 50 nL/min by using a nanoflow meter. Average flow rates in a 2-h run were 51.2 nL/min with RSD 0.7% and 21.0 nL/min with RSD 1.8%. Repeatability of analysis of the nanoHPLC/ESI-IT-TOF MS system was also tested by injecting 1.0 microL of trypsin digested bovine serum albumin (BSA) (100 fmol) into a monolithic silica-ODS column (30 microm i.d., 150 mm in length) through a packed silica-ODS trapping column (particle size 5 microm, 150 microm i.d., 10 mm in length). At a flow rate of 50 nL/min, the result demonstrated a reasonably good repeatability of peak retention times (RSD: 0.32-1.1%) and base-ion peak areas (RSD: 4.4-6.6%).
authors.affiliation: Hitachi High-Technologies Co., Hitachinaka 312-8504, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Ito
authors.forename: Shinji
authors.initials: S
authors.lastname: Yoshioka
authors.forename: Izumi
authors.initials: I
authors.lastname: Ogata
authors.forename: Eri
authors.initials: E
authors.lastname: Yamashita
authors.forename: Shinji
authors.initials: S
authors.lastname: Nagai
authors.forename: Toyoharu
authors.initials: T
authors.lastname: Okumoto
authors.forename: Kimihiko
authors.initials: K
authors.lastname: Ishii
authors.forename: Masahito
authors.initials: M
authors.lastname: Ito
authors.forename: Hironori
authors.initials: H
authors.lastname: Kaji
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Takao
authors.forename: Kisaburo
authors.initials: K
authors.lastname: Deguchi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods
chemical_list: D012710:Serum Albumin, Bovine