Collection of human genomic DNA from buccal cells for genetics studies: comparison between cytobrush, mouthwash, and treated card.
Alternative sources such as buccal cells have already been tested for genetic studies and epidemiological investigations. Thirty-seven volunteers participated in this study to compare cytology brushes, mouthwash, and treated cards for DNA collection. Quantity and quality of DNA and cost and feasibility were assessed. The mean DNA yield at 260 nm was found to be 3.5, 4, and 2.6 mu g for cytobrushes, mouthwashes, and treated cards, respectively. A second quantification technique by fluorescence showed differences in the DNA yield with 1.1 and 5.2 mu g for cytobrushes and mouthwash, respectively. All buccal samples allowed isolation of DNA suitable for polymerase chain reaction. According to the procedure of sample collection, the yield and purity of collected DNA, and storage conditions, the use of cytobrush appears to be the more appropriate method for DNA collection. This protocol has been validated and is currently applied in three large-scale multicentric studies including adults or children.
Mulot
Claire
C
Institut National de la Santé et de la Recherche Médicale, Unité 490,Toxicologie Moléculaire, Faculté des Saints Péres, Université René Descartes, Paris, France.
Stücker
Isabelle
I
Clavel
Jacqueline
J
Beaune
Philippe
P
Loriot
Marie-Anne
MA