Using LC/MS/MS to determine matrine, oxymatrine, ferulic acid, mangiferin, and glycyrrhizin in the Chinese medicinal preparations Shiau-feng-saan and Dang-guei-nian-tong-tang.
We have developed a simple, rapid, selective, and reproducible method for the quality control of traditional Chinese medicinal preparations. In this study, we used LC/MS/MS to simultaneously identify and quantify five marker compounds - matrine, oxymatrine, ferulic acid, mangiferin, and glycyrrhizin - in preparations of Shiau-feng-saan and Dang-guei-nian-tong-tang. The calibration curves for the five marker compounds were linear over the concentration range 50-2500 ng/mL (R2>0.9971). The matrix effect was minimized and the recoveries of the five marker compounds were >90% at a concentration of 1 microg/mL. Our experimental data reveal that significant differences exist between samples obtained from different sources.
Jong
Ting-Ting
TT
Department of Chemistry, National Chung-Hsin University, Taichung, Taiwan. ttjong@mail.nchu.edu.tw
Lee
Maw-Rong
MR
Chiang
Yi-Cheng
YC
Chiang
Shu-Tuan
ST
D000470:Alkaloids / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019695:Glycyrrhizic Acid / Q000032:analysis*; D011786:Quality Control; D011807:Quinolizines / Q000032:analysis*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors; D044004:Xanthones / Q000032:analysis*; D000093842:Matrines
D000470:Alkaloids; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; D044004:Xanthones; C013592:mangiferin; D019695:Glycyrrhizic Acid; C037573:oxymatrine; C004999:ferulic acid; D000093842:Matrines