Adaptor polymerase chain reaction for single molecule amplification.
The adaptor polymerase chain reaction (PCR) permits the amplification of DNA fragments with arbitrary sequences. In this paper, we describe the successful amplification of plasmid-derived single molecule DNAs digested by a restriction enzyme. By using adaptors made of short and long oligonucleotides, nonspecific interactions during PCR were suppressed. The method will be applicable to the detection of single molecule DNA fragments even if their sequence is unknown.
Nakano
Michihiko
M
Department of Ecological Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.
Komatsu
Jun
J
Kurita
Hirofumi
H
Yasuda
Hachiro
H
Katsura
Shinji
S
Mizuno
Akira
A
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*
D004247:DNA