[Microdetermination of proteins with Titan yellow by the resonance light scattering method].
A resonance light scattering method for the determination of trace proteins was developed. In the presence of Triton X-100, proteins reacted with Titan yellow to form a combination product, resulting a significant enhancement of resonance light scattering (RLS). The deltaI(RLS) was directly proportional to the concentration of protein in the range of 0.03-0.9 microg x mL(-1), with the detection limit 17.7 ng x mL(-1) for BSA. This method was applied to the determination of the proteins in synthetic and human serum samples, and compared to the CBB method, with satisfactory results.
Feng
Su-ling
SL
College of Chemistry and Environmental Science, Key Laboratory of Environmental Science and Engineering of Henan Education Department, Henan Normal University, Xinxiang 453002, China.
Wang
Jin
J
Fan
Jing
J
D000818:Animals; D002417:Cattle; D006801:Humans; D008027:Light; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000032:analysis / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014226:Triazenes / Q000737:chemistry*
D011506:Proteins; D012709:Serum Albumin; D014226:Triazenes; C044584:titan yellow; D012710:Serum Albumin, Bovine