The release of soluble VAP-1/SSAO by 3T3-L1 adipocytes is stimulated by isoproterenol and low concentrations of TNFalpha.
Plasma level of the protein VAP-1/SSAO (Vascular Adhesion Protein-1/Semicarbazide-Sensitive Amine Oxidase) is increased in diabetes and/or obesity and may be related to vascular complications associated to these pathologies. The aim of this work was to complete a preceding study where we described the role played by some hormones or metabolites, implicated in diabetes and/or obesity, in the regulation of the release of VAP-1/SSAO by 3T3-L1 adipocytes. Here we focused on the previously observed effect produced by TNFalpha in the release of VAP-1/SSAO and studied the effect of a beta-adrenergic compound, isoproterenol. Both compounds stimulated the release of VAP-1/SSAO to the culture medium but had a different effect on the VAP-1/SSAO membrane form. While TNFalpha produced a decrease on VAP-1/SSAO membrane form content, isoproterenol did not modify it. We thus observed two different ways of regulation of the release of VAP-1/SSAO by 3T3-L1 adipocytes by metabolites implicated in diabetes and adipose tissue physiopathology. Our work permits a better understanding of this increased plasma VAP-1/SSAO levels observed in diabetes.
García-Vicente
S
S
Parc Científic de Barcelona, Departament de Bioquica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Josep Samitier 1-5, E-08028 Barcelona, Spain.
Abella
A
A
Viguerie
N
N
Ros-Baró
A
A
Camps
M
M
Testar
X
X
Palacín
M
M
Zorzano
A
A
Marti
L
L
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D006631:Amine Oxidase (Copper-Containing) / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D018929:Cell Culture Techniques; D002458:Cell Fractionation; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D007545:Isoproterenol / Q000494:pharmacology*; D051379:Mice; D012995:Solubility; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D000318:Adrenergic beta-Agonists; D015815:Cell Adhesion Molecules; D014409:Tumor Necrosis Factor-alpha; D006631:Amine Oxidase (Copper-Containing); C492678:semicarbazide-sensitive amine oxidase-vascular adhesion protein-1, mouse; D007545:Isoproterenol