Influence of monocyte-like cells on the fibrinolytic activity of peritoneal mesothelial cells and the effect of sodium hyaluronate.
OBJECTIVE
To determine whether the presence of cells of the monocyte-macrophage system affects the fibrinolytic response of peritoneal mesothelial cells to lipopolysaccharide (LPS) in the presence and absence of sodium hyaluronate.


METHODS
Controlled laboratory experiment.


METHODS
Cell cultures in an academic laboratory research environment.


METHODS
Human peritoneal mesothelial cells were harvested from patients undergoing a laparotomy for noninfectious reasons and were cultured in vitro. Co-cultures were formed by adding U-937 human monocyte-like cells to a monolayer of mesothelial cells.


METHODS
After 24 hours, cultures were treated with 10 ng/mL of LPS, and sodium hyaluronate was added in a final concentration of 0.2%. Controls received medium without sodium hyaluronate.


METHODS
After 24 hours' incubation, tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), and plasminogen activator inhibitor-1 (PAI-1) levels were determined in medium and cell lysates by using ELISA techniques.


RESULTS
In medium of co-cultures, tPA and PAI-1 concentrations were statistically significantly increased compared with the case of monocultures, whereas uPA concentration was statistically significantly decreased. In cell lysates of co-cultures, PAI-1 concentration was statistically significantly increased compared with the case of monocultures, whereas tPA and uPA were unaffected. Treatment with sodium hyaluronate statistically significantly decreased PAI-1 and uPA concentrations in medium of monocultures but decreased uPA concentration only in medium of co-cultures, compared with the case of controls.


CONCLUSIONS
Cells of the monocyte-macrophage system modulate the fibrinolytic capacity of LPS treated human peritoneal mesothelial cells and interfere in the hyaluronan-associated changes in mesothelial fibrinolytic capacity.
Sikkink
Cornelis J J M
CJ
Department of Surgery, University Medical Center St. Radboud, Nijmegen, The Netherlands.
Reijnen
Michel M P J
MM
Falk
Peter
P
van Goor
Harry
H
Holmdahl
Lena
L
D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D005342:Fibrinolysis / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D010537:Peritoneum / Q000187:drug effects / Q000378:metabolism*; D020298:U937 Cells
D008070:Lipopolysaccharides; D006820:Hyaluronic Acid