title: [Direct bone morphogenetic protein 2 gene therapy for repairing segmental radial defect in rabbits].
abstract: OBJECTIVE
  To study the effect of direct bone morphogenetic protein 2 (BMP-2) gene therapy mediated by adenovirus on repairing bone defect.
  
  
  METHODS
  The radial defect models were made on 60 rabbits, which were evenly divided into 4 groups randomly. The 4 groups were treated with different materials: group A, adenovirus carrying BMP-2 gene (Ad-BMP-2) plus bovine cancellous bone (BCB); group B, reconstructed BMP-2 plus BCB; group C, Ad-Lacz plus BCB; and group D, only BCB scaffolds. The X-ray, histological examination, biomechanics analysis, and immunohistochemical staining were made 4, 8, and 12 weeks after the operation.
  
  
  RESULTS
  Group A gained better effect in the volume of new bones, the anti-bending intensity of the healing bone, and the expression of BMP-2 than those of group B. The defect in group A was healed. No new bones were observed in group C and group D.
  
  
  CONCLUSIONS
  Direct BMP-2 gene therapy is easy to perform and has very strong osteo-induction ability. It is a good method to repair segmental bone defects.
authors.affiliation: Department of Orthopaedics, the Second Hospital of China Medical University, Liaoning Shenyang, 110003, P. R. China. lijianjun71@yahoo.com.cn
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Lunhao
authors.initials: L
authors.lastname: Bai
authors.forename: Hongbin
authors.initials: H
authors.lastname: Sun
authors.forename: Dong
authors.initials: D
authors.lastname: Han
authors.forename: Jiaxiang
authors.initials: J
authors.lastname: Gu
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.forename: Jingzhu
authors.initials: J
authors.lastname: Duan
authors.forename: Xinxiang
authors.initials: X
authors.lastname: Xu
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics*; D001861:Bone Regeneration; D018786:Bone Substitutes; D016025:Bone Transplantation; D002417:Cattle; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D011817:Rabbits; D011884:Radius / Q000293:injuries* / Q000473:pathology / Q000601:surgery; D011897:Random Allocation; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes