Single multiplex PCR assay to identify simultaneously the six categories of diarrheagenic Escherichia coli associated with enteric infections.
We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx(1), stx(2), and eae), enteropathogenic (eae and bfp), enterotoxigenic (st II and lt), enteroinvasive (vir F and ipa H), entero-aggregative (aaf II), and diffuse adherent (daa E) Escherichia coli in stool samples.
Vidal
Maricel
M
Programa de Microbiología, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile. rvidal@med.uchile.cl
Kruger
Eileen
E
Durán
Claudia
C
Lagos
Rosanna
R
Levine
Myron
M
Prado
Valeria
V
Toro
Cecilia
C
Vidal
Roberto
R
D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology*; D004768:Enterotoxins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins