Preparation of anti-idiotypic antibody against avian influenza virus subtype H9.
To generate monoclonal anti-idiotypic antibodies (mAb2) against avian influenza virus subtype H9 (H9 AIV), BALB/c mice were immunized with purified chicken anti-H9-AIV IgG and the splenocytes of immunized mice were fused with myeloma cells NS-1. Hybridoma cells were screened by indirect enzyme-linked immunosorbent assays with both chicken and rabbit anti-H9-AIV IgG as coating antigens. One hybridoma cell clone secreting monoclonal antibody against idiotypes shared by both chicken and rabbit anti-H9-AIV IgG was established. Experiments demonstrated the mAb2 was able to inhibit the binding of hemagglutinin to anti-H9-AIV IgG and to induce chickens to generate hemagglutination inhibition antibodies, indicating this anti-species-sharing-idiotypic antibody bore the internal image of hemagglutinin on avian influenza virus.
Li
Baoquan
B
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Peng
Jun
J
Niu
Zhongxiang
Z
Yin
Xunhe
X
Liu
Faxiao
F
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000302:isolation & purification*; D002645:Chickens; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006825:Hybridomas / Q000276:immunology; D066298:In Vitro Techniques; D009980:Influenza A virus / Q000145:classification* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral