Investigation of genotoxic and antigenotoxic activities of Melampodium divaricatum in Salmonella typhimurium.
Melampodium divaricatum is a member of the Asteraceae and in Brazil is known as false-calendula, its flowers being used in anti-inflammatory preparations, substituting the true calendula or marigold (Calendula officinalis L.). The flower extract was investigated for mutagenic and antimutagenic effect in the Salmonella/microsome assay. The tested extract was not mutagenic in the strains TA100, TA98, TA97a and TA102 and decreased the mutagenicity of aflatoxin B1, benzo(a)pyrene and daunomycin. Chlorophyll and triterpenes were detected in the extract, and they might have contributed to the observed effect. Our data suggest that these medicinal plants possess cancer chemopreventive properties.
Nogueira
Maira Eiko Ikuma
ME
Department of Biological Sciences, Faculty of Pharmaceutical Sciences of Araraquara, Estadual Paulist University, UNESP-Rodovia Araraquara-Jaú km 1, 14801-902 Araraquara, SP, Brazil.
Passoni
Mariana Henrique
MH
Biso
Fabiana Izilda
FI
Longo
Maria do Carmo
Mdo C
Cardoso
Cássia Regina Primila
CR
Santos
Lourdes Campaner Dos
LC
Varanda
Eliana Aparecida
EA
D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors / Q000633:toxicity; D000818:Animals; D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors / Q000633:toxicity; D016587:Antimutagenic Agents / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D001564:Benzo(a)pyrene / Q000037:antagonists & inhibitors / Q000633:toxicity; D002734:Chlorophyll / Q000633:toxicity; D002855:Chromatography, Thin Layer; D004249:DNA Damage / Q000187:drug effects; D003630:Daunorubicin / Q000037:antagonists & inhibitors / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D035264:Flowers / Q000737:chemistry; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation / Q000187:drug effects / Q000235:genetics; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics*; D014315:Triterpenes / Q000633:toxicity
D000900:Anti-Bacterial Agents; D016587:Antimutagenic Agents; D009153:Mutagens; D010936:Plant Extracts; D014315:Triterpenes; D002734:Chlorophyll; D001564:Benzo(a)pyrene; D016604:Aflatoxin B1; D003630:Daunorubicin