Microarray methods in Drosophila neurobiology.
In the relatively short period since their development, DNA microarrays have been used increasingly in the study of genetic and cellular processes, thereby offering a genome-wide approach to gene expression studies. With the advent of genome sequencing programs for organisms from yeast to man, the number of organisms which now have ready-made commercial arrays continues to increase. Here, the principle of DNA microarrays is introduced, with particular attention being given to the role of this technology in studies of the nervous system of the fruitfly Drosophila melanogaster. The importance of experimental design and sample preparation, in line with minimum information about microarray experiment (MIAME) compliance, is emphasised. The technical platforms available to the Drosophila neurobiologist have been illustrated and a brief number of data analysis tools that are readily available reviewed.
Mee
Christopher J
CJ
MRC Functional Genetics Unit, Dept. of Human Anatomy and Genetics, University of Oxford, South Parks Road, OX1 3QX, Oxford, UK. christopher.mee@anat.ox.ac.uk
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D017344:Genes, Insect / Q000235:genetics*; D009446:Neurobiology / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*