title: A carbon fiber microelectrode-based third-generation biosensor for superoxide anion.
abstract: Implantable and miniature carbon fiber microelectrode (CFME)-based third-generation biosensor for superoxide anion (O(2)(-)) was fabricated for the first time. The CFME-based biosensor was constructed by electro-deposition of Au nanoparticles on the CFMEs and then modification of the Au nanoparticles by cysteine followed by immobilization of superoxide dismutase (SOD) on the electrodes. The direct electrochemistry of the SOD immobilized on the CFME-based electrodes was efficiently realized by electron transfer promoter - cysteine molecules confined on the Au nanoparticles deposited on the CFMEs. The CFME-based biosensors were demonstrated to possess striking analytical properties for O(2)(-) determination, such as optional operation potentials, high selectivity and sensitivity as well as good stability. Along with the implantable capacity inherent in the CFMEs, these striking analytical properties of the CFME-based biosensors substantially make them potential for in vivo determination of O(2)(-).
authors.affiliation: Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan.
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
authors.forename: Lanqun
authors.initials: L
authors.lastname: Mao
authors.forename: Takeyoshi
authors.initials: T
authors.lastname: Okajima
authors.forename: Takeo
authors.initials: T
authors.lastname: Ohsaka
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008839:Microelectrodes; D013482:Superoxide Dismutase / Q000737:chemistry*; D013481:Superoxides / Q000032:analysis* / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000077482:Carbon Fiber; D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D013481:Superoxides; D002244:Carbon; D013482:Superoxide Dismutase