GABA and glutamate transporters are expressed in human platelets.
GABA and glutamate are the major neurotransmitters in the human central nervous system. Disturbances in these transmitter systems have been suggested to influence a variety of neurological and psychiatric diseases. Human platelets have been used as a model for neural amino acid transport, although it has not been known exactly which transporters participate in the transport process. In this study, we identify with reverse transcription-polymerase chain reaction (RT-PCR) BGT-1 and EAAT3 as transporters for GABA and glutamate, respectively. We also show that platelets contain transporters for dopamine, taurine and creatine. The cloning of these transporters confirms that blood platelets can be used as a model for neurotransmitter transport in the CNS.
Rainesalo
Sirpa
S
Department of Neurology and Rehabilitation, Tampere University Hospital, Tampere, Finland.
Keränen
Tapani
T
Saransaari
Pirjo
P
Honkaniemi
Jari
J
D000328:Adult; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D050483:Dopamine Plasma Membrane Transport Proteins / Q000097:blood / Q000235:genetics; D050580:Excitatory Amino Acid Transporter 3 / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D050485:GABA Plasma Membrane Transport Proteins; D018698:Glutamic Acid / Q000097:blood*; D006801:Humans; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000097:blood / Q000235:genetics; D026901:Membrane Transport Proteins / Q000097:blood / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D005680:gamma-Aminobutyric Acid / Q000097:blood*; D000099247:Taurine Transporters
D002352:Carrier Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; D050580:Excitatory Amino Acid Transporter 3; D050485:GABA Plasma Membrane Transport Proteins; D018698:Glutamic Acid; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D005680:gamma-Aminobutyric Acid; D000099247:Taurine Transporters; C493400:SLC1A1 protein, human; C080679:creatine transporter; C072207:betaine plasma membrane transport proteins