title: Otago Diagnostic Laboratories' (ODL) Method for the detection of beta-lactamases in Enterobacteriaceae.
abstract: OBJECTIVE
  The rapid evolvement of beta-lactamases in Enterobacteriaceae is an important concern and the clinical microbiology laboratory is required to detect them, where possible, using a rapid, reliable, simple and low cost methodology.
  
  
  METHODS
  A disc diffusion method using NCCLS breakpoints, Jarlier's principle and cefoxitin test for AmpC was carried out. It incorporated seven antimicrobial discs in one agar plate: cefotaxime, aztreonam, amoxicillin-clavulanate, ceftazidime, cefpodoxime, cefepime and cefoxitin. NCCLS disc confirmation test for extended-spectrum beta-lactamase (ESBL) was carried out simultaneously.
  
  
  RESULTS
  AmpC, ESBL, CTX-M, and K1 were detected using these tests. The prevalence of ESBL was <1% in the hospital.
  
  
  CONCLUSIONS
  The method is recommended for the phenotypic detection of beta-lactamases in Enterobacteriaceae or for confirmation after the results are obtained by conventional automated systems.
authors.affiliation: Otago Diagnostic Laboratories, Dunedin Hospital, Otago District Health Board, New Zealand.
authors.forename: C T W
authors.initials: CT
authors.lastname: Han
authors.forename: K D
authors.initials: KD
authors.lastname: Parnell
authors.forename: Y F
authors.initials: YF
authors.lastname: Chiew
authors.forename: J Y H
authors.initials: JY
authors.lastname: Lin
authors.forename: P
authors.initials: P
authors.lastname: Turner
authors.forename: A S
authors.initials: AS
authors.lastname: Wilson
authors.forename: J D
authors.initials: JD
authors.lastname: Davies
authors.forename: J A
authors.initials: JA
authors.lastname: Robinson
authors.forename: A M
authors.initials: AM
authors.lastname: de Ruyter
authors.forename: K M
authors.initials: KM
authors.lastname: Thompson
authors.forename: J A
authors.initials: JA
authors.lastname: Moodie
authors.forename: M C
authors.initials: MC
authors.lastname: Johnstone
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects / Q000201:enzymology*; D004756:Enterobacteriaceae Infections; D006801:Humans; D007755:Laboratories, Hospital; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009520:New Zealand; D010641:Phenotype; D001618:beta-Lactamases / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases