"Green" route to 6-hydroxy-3-succinoyl-pyridine from (S)-nicotine of tobacco waste by whole cells of a Pseudomonas sp.
A new technology for 6-hydroxy-3-succinoyl-pyridine (HSP) production from (S)-nicotine in tobacco waste by whole cells of a Pseudomonas sp. has been developed. When deionized water was used in the transformation reaction as a medium and the initial pH value of reaction mixture was adjusted to 7.0, 1.45 g/L HSP was produced from 3 g/L of nicotine in 5 h with 3.4 g/L of cells in a 5-L flask at 30 degrees C. HSP could be easily purified from the reaction without perplexing separation steps. A quantity of 1.3 g of HSP was recovered without impurity, and the overall yield of HSP was 43.8% (w/w), based on an initial concentration of 3.0 g/L of nicotine in reaction. This biotransformation made it possible to convert nicotine in tobacco wastes with high nicotine content into valuable compounds.
Wang
Shu Ning
SN
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Xu
Ping
P
Tang
Hong Zhi
HZ
Meng
Jing
J
Liu
Xiao Lei
XL
Qing
Cui
C
D001711:Biotransformation; D002855:Chromatography, Thin Layer; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D009538:Nicotine / Q000737:chemistry / Q000378:metabolism*; D011549:Pseudomonas / Q000166:cytology / Q000378:metabolism*; D011725:Pyridines / Q000737:chemistry / Q000378:metabolism*; D013386:Succinates / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana / Q000737:chemistry*; D014028:Tobacco Smoke Pollution / Q000517:prevention & control*; D014867:Water / Q000737:chemistry
C507268:6-hydroxy-3-succinoyl-pyridine; D007220:Industrial Waste; D011725:Pyridines; D013386:Succinates; D014028:Tobacco Smoke Pollution; D014867:Water; D009538:Nicotine