[The transformation of fibroblast-like cells originating from different tissue into osteoblasts].
OBJECTIVE
To discuss whether fibroblast-like cells can transform into osteoblasts under specific condition during distraction osteogenesis.


METHODS
Fibroblast-like cells were cultured in vitro. BMP-2, TGF-beta(1), BMP-2 and TGF-beta(1) were added into the medium, and the cellular change were observed by histochemistry and immunohistochemistry methods. The distraction was given on the membrane of fibroblast-like cells, and the cellular change were observed under the distraction.


RESULTS
fibroblast-like cells from the joint could transform into osteoblasts under the effect of BMP-2 or BMP-2 with TGF-beta(1). Fibroblast-like cells from other regions could not transform into osteoblasts.


CONCLUSIONS
The osteoblasts during distraction osteogenesis are multi-origin.
Luo
Quan-feng
QF
Department of Oral and Maxillofacial Surgery, Peking University School of Stomatology, Beijing 100081, China.
Wang
Xing
X
Yi
Biao
B
Li
Zi-li
ZL
Wang
Xiao-xia
XX
Liang
Cheng
C
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D019857:Osteogenesis, Distraction; D011817:Rabbits; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
D055396:Bone Morphogenetic Protein 2; D053773:Transforming Growth Factor beta1