Fluorescence enhancement effect of the morin-Al3+ -sodium dodecyl benzene sulphonate-protein system and the determination of proteins.
The fluorescence intensity of the morin-Al(3+) complex was greatly enhanced by proteins in the presence of sodium dodecyl benzene sulphonate (SDBS). Based on this, a new fluorimetric method for the determination of protein was developed. Under optimum conditions, the enhanced intensity of fluorescence was in proportion to the concentration of proteins in the range 1.0 x 10(-8)-1.3 x 10(-5) g/mL for bovine serum albumin (BSA), 4.0 x 10(-8)-1.2 x 10(-5) g/mL for egg albumin (EA) and 5.0 x 10(-8)-1.2 x 10(-5) g/mL for human serum albumin (HSA). Their detection limits (S:N = 3) were 5.0 x 10(-9), 1.8 x 10(-8) and 1.6 x 10(-8) g/mL, respectively. The interaction mechanism was also studied.
Wang
Fei
F
Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China.
Yang
Jinghe
J
Wu
Xia
X
Sun
Changxia
C
Liu
Shufang
S
Wang
Feng
F
Jia
Zhen
Z
D000535:Aluminum / Q000737:chemistry*; D001557:Benzenesulfonates / Q000737:chemistry*; D002021:Buffers; D002138:Calibration; D005419:Flavonoids / Q000737:chemistry*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry; D013501:Surface-Active Agents; D047309:Flavones
D000535:Aluminum; D001557:Benzenesulfonates; D002021:Buffers; D005419:Flavonoids; D012710:Serum Albumin, Bovine; D013501:Surface-Active Agents; C001114:dodecylbenzenesulfonic acid; C008548:morin; D047309:Flavones