The inhibition of osteogenesis with human bone marrow mesenchymal stem cells by CdSe/ZnS quantum dot labels.
CdSe/ZnS quantum dots (QDs) have recently been used as cell tracers for long term imaging of live cells. A number of studies indicate that introduction of quantum dots to cells have no apparent deleterious effects on the morphology or growth of cells. In the present study, the human bone marrow mesenchymal stem cells (hBMSCs) were used as a model to examine the effects of QDs on the growth and osteogenic differentiation of the cells. The CdSe/ZnS QDs were delivered into hBMSCs by liposome-mediated transfection with high efficiency; analysis by transmission electron microscopy revealed that the internalized QDs could be located in the endosome-like vesicles. Uptake of QDs into hBMSCs did not affect the proliferation and cell cycle distribution of the cells. When induced to differentiate along the osteogenic lineage, the QD-containing-hBMSCs were shown to have mineral deposition on the extracellular matrix. However, the cells displayed lower alkaline phosphatase activity as compared to those without QDs. Analysis by reverse transcriptase polymerase chain reaction further demonstrated that the expression of two osteogenic markers, osteopontin and osteocalcin, was significantly inhibited. Together our results show that the presence of QDs in hBMSCs prevents the full response of the cells to induced osteogenic differentiation.
Hsieh
Shu-Chen
SC
Institute of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan.
Wang
Fung-Fang
FF
Lin
Ching-Shih
CS
Chen
Yu-Ju
YJ
Hung
Shih-Chieh
SC
Wang
Yng-Jiin
YJ
D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D019187:Cadmium Compounds / Q000378:metabolism* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D045663:Quantum Dots; D018036:Selenium Compounds / Q000378:metabolism* / Q000494:pharmacology; D013194:Staining and Labeling; D013440:Sulfides / Q000378:metabolism* / Q000494:pharmacology; D017967:Zinc Compounds / Q000378:metabolism* / Q000494:pharmacology
D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C058667:cadmium selenide; C031238:zinc sulfide