title: Effect of cold storage on cholinergic responses induced by electrical field stimulation in human bronchi.
abstract: The aim of the present study was to examine the effects of cold storage on the responses induced by electrical field stimulation (EFS) in human bronchial preparations. Responses induced by EFS and acetylcholine were studied in human bronchial rings mounted in organ baths, either on the day of surgery or after storage at 4 degrees C in Krebs-Henseleit solution for 24 and 48 h, respectively. The responses induced by EFS were studied at different voltages (20, 40 and 60 V) and at a range of frequencies (2, 4, 8, 10, 30 and 60 Hz). EFS induced a triphasic response, consisting of a cholinergic contraction, followed by a relaxation and subsequently a slow sustained contraction. The amplitude of the EFS-induced response was enhanced with increasing voltages and increasing frequencies. None of the three EFS-induced phases were significantly altered by cold storage at 24h, whereas storage for 48 h significantly decreased the reactivity of the preparations. Likewise, the contractions induced by acetylcholine were unaltered after 24h, but significantly depressed after 48 h. These results suggest that the reactivity of human bronchial preparations to EFS is not altered when tissues are conserved for 24h, whereas prolonged storage should be avoided.
authors.affiliation: Center for Molecular Medicine, Karolinska University Hospital, L8:03, 171 76 Stockholm, Sweden. magnus.back@cmm.ki.se
authors.forename: Magnus
authors.initials: M
authors.lastname: Bäck
authors.forename: Maria
authors.initials: M
authors.lastname: Costantino
authors.forename: Charles
authors.initials: C
authors.lastname: Brink
authors.forename: Xavier
authors.initials: X
authors.lastname: Norel
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D001285:Atropine / Q000494:pharmacology; D001980:Bronchi / Q000187:drug effects / Q000294:innervation / Q000502:physiology*; D016084:Bronchoconstriction / Q000187:drug effects / Q000502:physiology; D002799:Cholinergic Fibers / Q000187:drug effects / Q000502:physiology*; D003080:Cold Temperature; D004558:Electric Stimulation; D004594:Electrophysiology; D006801:Humans; D009388:Neostigmine / Q000494:pharmacology; D012034:Refrigeration; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors; D014021:Tissue Preservation
chemical_list: D009388:Neostigmine; D013779:Tetrodotoxin; D001285:Atropine; D000109:Acetylcholine