Melatonin reduces inflammatory injury through inhibiting NF-kappaB activation in rats with colitis.
Proinflammatory mediators are important in the pathogenesis of IBD, which are regulated by activation of NF-kappaB. The aim of this study was to investigate whether melatonin reduces inflammatory injury and inhibits proinflammatory molecule and NF-kappaB in rats with colitis. Rat colitis model was established by TNBS enema. NF-kappaB p65, TNF-alpha, ICAM-1, and IkappaBalpha in colon tissue were examined by immunohistochemistry, EMSA, RT-PCR, and Western blot analysis. Expression of proinflammatory molecule and activation of NF-kappaB were upregulated and IkappaB level decreased in rats with colitis. Melatonin reduces colonic inflammatory injury through downregulating proinflammatory molecule mediated by NF-kappaB inhibition and blockade of IkappaBalpha degradation.
Li
Jun-Hua
JH
Department of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Province, Wuhan, China.
Yu
Jie-Ping
JP
Yu
Hong-Gang
HG
Xu
Xi-Ming
XM
Yu
Liang-Liang
LL
Liu
Jin
J
Luo
He-Sheng
HS
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects*; D005260:Female; D020672:I-kappa B Proteins / Q000096:biosynthesis; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D008297:Male; D008550:Melatonin / Q000008:administration & dosage*; D000072000:NF-KappaB Inhibitor alpha; D051381:Rats; D017207:Rats, Sprague-Dawley; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015854:Up-Regulation / Q000187:drug effects
D000975:Antioxidants; D020672:I-kappa B Proteins; C000606206:Nfkbia protein, rat; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D000072000:NF-KappaB Inhibitor alpha; D008550:Melatonin