Attaching single biomolecules selectively to the apex of AFM tips for measuring specific interactions.
We present a general approach for preparing well-defined AFM tips for probing single target molecules. We demonstrated that carboxylic acid groups could be generated by electrochemical oxidation selectively at the apex of an AFM tip that is coated with a monolayer of oligo(ethylene glycol) derivatives for resisting nonspecific interactions. These carboxylic acid groups were used as handles to tether only one ligand molecule, such as biotin, to the tip apex for measurement of specific interactions with biomolecules.
Gu
Jianhua
J
Department of Chemistry & Center for Materials Chemistry, University of Houston, Houston, TX 77204-5003, USA.
Xiao
Zhongdang
Z
Yam
Chi-Ming
CM
Qin
Guoting
G
Deluge
Maxence
M
Boutet
Sabine
S
Cai
Chengzhi
C
D001672:Biocompatible Materials / Q000737:chemistry; D001703:Biophysics / Q000379:methods; D002264:Carboxylic Acids / Q000737:chemistry; D004563:Electrochemistry; D019855:Ethylene Glycol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods*; D008956:Models, Chemical; D013314:Stress, Mechanical; D000267:Tissue Adhesions
D001672:Biocompatible Materials; D002264:Carboxylic Acids; D008024:Ligands; D019855:Ethylene Glycol