Adsorption behavior of antimicrobial peptide histatin 5 on PMMA.
Adsorption of antimicrobial peptide histatin 5 on a poly(methyl methacrylate) denture base may serve to prevent biofilm formation, leading to a reduction of denture-induced stomatitis. This study focused on adsorption behavior of histatin 5 onto PMMA surfaces modified using a cold plasma technique and the effectiveness of histatin 5 adsorption for reducing Candida albicans biofilm formation by the quartz crystal microbalance-dissipation (QCM-D) technique. PMMA spin-coated specimens were treated with oxygen (O(2)) plasma using a plasma surface modification apparatus. The amount of histatin 5 adsorbed onto the PMMA treated with O(2) plasma is more than six times greater than that adsorbed onto untreated PMMA. The degree of histatin 5 adsorption had a negative correlation with the contact angle, whereas that of zeta-potential showed no significant correlation. XPS analysis revealed that the introduction of the carboxyl and O(2) functional groups were observable on the O(2) plasma-treated PMMA. Increased surface hydrophilicity and the formation of the carboxyl could be responsible for histatin 5 adsorption on plasma-treated PMMA. There is no significant difference between histatin-adsorbed PMMA and control PMMA for C. albicans initially attached. On the contrary, the amount of C. albicans colonization on histatin-adsorbed PMMA was significantly less than the control.
Yoshinari
Masao
M
Department of Dental Materials Science and Oral Health Science Center, Tokyo Dental College, 1-2-2 Masago, Chiba 261-8502, Japan. yosinari@tdc.ac.jp
Kato
Tetsuo
T
Matsuzaka
Kenichi
K
Hayakawa
Tohru
T
Inoue
Takashi
T
Oda
Yutaka
Y
Okuda
Katsuji
K
Shimono
Masaki
M
D000327:Adsorption; D000935:Antifungal Agents / Q000737:chemistry*; D018441:Biofilms; D001843:Bone Cements / Q000737:chemistry*; D002176:Candida albicans / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D054808:Histatins; D006801:Humans; D008422:Materials Testing; D010100:Oxygen / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D013499:Surface Properties
D000935:Antifungal Agents; D001843:Bone Cements; D020099:Coated Materials, Biocompatible; C068075:HTN3 protein, human; D054808:Histatins; D012471:Salivary Proteins and Peptides; D019904:Polymethyl Methacrylate; D010100:Oxygen