Poly-L-lysine-modified silica nanoparticles: a potential oral gene delivery system.
Poly-L-lysine-modified silica nanoparticles (PMS-NP) is a novel nonviral vector for gene delivery, which can efficiently deliver plasmid DNA and antisense oligonucleotides into cultured cells in vitro in the presence of serum-free medium. However, little is known about whether PMS-NP is a suitable carrier for gene delivery by oral administration. To this end, oral gene delivery assays were performed, and glucose transporting tests showed that PMS-NP had no obvious toxicity to intestine of BALB/C mice. Efficient reporter gene expression was detected in stomach and intestine where expression was mainly observed in mucous membrane cells. These results indicated that PMS-NP was a low-toxicity carrier, hence demonstrating its potential for fundamental research and gene therapy, especially for oral gene therapy.
Li
Zheng
Z
Cancer Research Institute, Central South University, Changsha 410078, P R. China.
Zhu
Shiguo
S
Gan
Kai
K
Zhang
Qiuhong
Q
Zeng
Zhaoyang
Z
Zhou
Yanhong
Y
Liu
Huaying
H
Xiong
Wei
W
Li
Xiaoling
X
Li
Guiyuan
G
D000284:Administration, Oral; D000818:Animals; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics; D016503:Drug Delivery Systems; D015870:Gene Expression; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D007422:Intestines / Q000201:enzymology; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D049329:Nanostructures; D011107:Polylysine / Q000633:toxicity; D012822:Silicon Dioxide / Q000633:toxicity; D013270:Stomach / Q000201:enzymology
D004274:DNA, Recombinant; D011107:Polylysine; D012822:Silicon Dioxide; D008156:Luciferases