Preparation of arsenic trioxide albumin microspheres and its release characteristics in vitro.
Arsenic trioxide albumin microspheres (As2O3-BSA-NS) were prepared by using methods of chemical cross-linking. The desirability function (DF), calculated according to the size (<1 microm) distribution, drug loading and drug trapping efficiency, was introduced as a total index for the microspheres formulation. Four factors, inculding W/O ratio, decentralization speed, BSA concentration and stirring stabilization time, were selected and arranged in an orthogonal experimental table. The release characteristic was studied by the drug release experiment in vitro. The four factors affected DF differently. Decentralization speed behaved as the maximum (P<0.01), followed by BSA concentration (P<0.05) and the W/O ratio dose (P<0.05). Stirring stabilization time did not influence DF (P>0.05). The release experiment in vitro showed that As2O3 in As2O3-BSA-NS was released more slower than pure As2O3. It was concluded that regular As2O3-BSA-NS may be prepared by the methods of chemical cross-linking, which was optimized by orthogonal experimental analysis of different factors, and the microspheres can release As2O3 slowly.
Zhou
Jie
J
Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science, and Technology, Wuhan 430022, China.
Zeng
Fuqing
F
Xiang
Gao
G
Xie
Shusheng
S
Wei
Shuli
S
D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D008863:Microspheres; D010087:Oxides / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013678:Technology, Pharmaceutical
D001152:Arsenicals; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D010087:Oxides; D012710:Serum Albumin, Bovine; D000077237:Arsenic Trioxide