Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic beta cells.
Several nuclear and cytoplasmic proteins in metazoans are modified by O-linked N-acetylglucosamine (O-GlcNAc). This modification is dynamic and reversible similar to phosphorylation and is catalyzed by the O-linked GlcNAc transferase (OGT). Hyperglycemia has been shown to increase O-GlcNAc levels in pancreatic beta cells, which appears to interfere with beta-cell function. To obtain a better understanding of the role of O-linked GlcNAc modification in beta cells, we have isolated OGT interacting proteins from a cDNA library made from the mouse insulinoma MIN6 cell line. We describe here the identification of Ataxin-10, encoded by the SCA10 (spinocerebellar ataxia type 10) gene as an OGT interacting protein. Mutations in the SCA10 gene cause progressive cerebellar ataxias and seizures. We demonstrate that SCA10 interacts with OGT in vivo and is modified by O-linked glycosylation in MIN6 cells, suggesting a novel role for the Ataxin-10 protein in pancreatic beta cells.
Andrali
Sreenath S
SS
Department of Molecular and Cellular Biochemistry, University of Kentucky, College of Medicine, 741 South Limestone Street, Lexington, KY 40536, USA.
März
Pia
P
Ozcan
Sabire
S
D000818:Animals; D000067736:Ataxin-10; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007515:Islets of Langerhans / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D025941:Protein Interaction Mapping
D000067736:Ataxin-10; C505248:Atxn10 protein, mouse; D002352:Carrier Proteins; D017351:N-Acetylglucosaminyltransferases; C063057:UDP-N-acetylglucosamine-peptide beta-N-acetylglucosaminyltransferase; D005947:Glucose