Heterogeneity of IgG glycosylation in adult periodontal disease.
Periodontal disease is a chronic inflammatory disease of bacterial etiology. In many other chronic inflammatory diseases, IgG glycans are galactose-deficient and thus capable of complement activation through the lectin pathway. In this study, we examined whether IgG in serum and gingival crevicular fluid, and IgG locally produced by plasma cells in gingiva of periodontal disease patients, display altered glycosylation. We developed a lectin-ELISA to measure levels of galactose-deficient IgG in the fluids and immunofluorescence staining to detect galactose-deficient IgG-producing cells in gingiva. Our results indicated higher levels of galactose-deficient IgG in sera and gingival crevicular fluid from periodontal disease patients, compared with levels in healthy controls. Furthermore, gingivae from periodontal disease patients exhibited infiltration of IgG-producing plasma cells; many of them contained galactose-deficient IgG in the cytoplasm. Analysis of our data suggests that IgG secreted by B-cells was aberrantly glycosylated, which resulted in the production of pro-inflammatory galactose-deficient IgG.
Novak
J
J
Department of Microbiology, University of Alabama at Birmingham, 845 19th Street South, Birmingham, AL 35294-2170, USA. jannovak@uab.edu
Tomana
M
M
Shah
G R
GR
Brown
R
R
Mestecky
J
J
D000328:Adult; D000368:Aged; D005260:Female; D005883:Gingival Crevicular Fluid / Q000276:immunology* / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000276:immunology* / Q000378:metabolism; D010514:Periodontal Pocket / Q000276:immunology / Q000378:metabolism; D010519:Periodontium / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D010950:Plasma Cells / Q000166:cytology* / Q000378:metabolism; D011487:Protein Conformation; D013223:Statistics as Topic
D007074:Immunoglobulin G