Feline coronavirus serotypes 1 and 2: seroprevalence and association with disease in Switzerland.
To determine the prevalence of antibodies to feline coronavirus (FCoV) serotypes 1 and 2 in Switzerland and their association with different disease manifestations, a serological study based on immunofluorescence tests was conducted with Swiss field cats using transmissible gastroenteritis virus (TGEV), FCoV type 1 and FCoV type 2 as antigens. A total of 639 serum samples collected in the context of different studies from naturally infected cats were tested. The current study revealed that, with an apparent prevalence of 83%, FCoV serotype 1 is the most prevalent serotype in Switzerland. FCoV type 1 viruses induced higher antibody titers than FCoV type 2, and were more frequently associated with clinical signs and/or feline infectious peritonitis. The antibody development in seven cats experimentally infected with FCoV type 1 revealed that, with progressing duration of infection, antibodies to FCoV type 1 significantly increased over those to FCoV type 2. There was a significant relationship between antibody titers against TGEV, FCoV 1, and FCoV 2 and TGEV antigen detected the highest proportion of seropositive cats. We conclude that a vaccine against FCoV should be based on FCoV type 1-related antigens and that for serodiagnosis of FCoV infection TGEV should be used to attain the highest diagnostic efficiency. When serology is used in addition to clinical signs, hematology, and clinical chemistry results as an aid to diagnose clinical FIP, TGEV shows a diagnostic efficiency equal to that of a FCoV antigen.
Kummrow
Maya
M
Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zürich, Switzerland.
Meli
Marina L
ML
Haessig
Michael
M
Goenczi
Enikoe
E
Poland
Amy
A
Pedersen
Niels C
NC
Hofmann-Lehmann
Regina
R
Lutz
Hans
H
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D000956:Antigens, Viral; D002415:Cats; D018352:Coronavirus Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000662:veterinary / Q000821:virology; D016765:Coronavirus, Feline / Q000145:classification / Q000302:isolation & purification*; D018450:Disease Progression; D016766:Feline Infectious Peritonitis / Q000175:diagnosis / Q000453:epidemiology; D015200:Fluoroimmunoassay; D015995:Prevalence; D013557:Switzerland
D000914:Antibodies, Viral; D000956:Antigens, Viral