[Preliminary study on anti-periodontitis immunization with DNA vaccine].
OBJECTIVE
To observe the protection against periodontal bone loss in the Sprague-Dawley (SD) rats periodontitis model, with the recombined plasmid pcDNA3.1+/kgpcd as DNA gene vaccine.


METHODS
PcDNA3.1+/kgpcd was delivered into rats by submandibular gland-targeted injection. The anti-KGPcd sIgA in saliva was measured by indirect ELISA method. Immunohistochemistry staining was used to assess the protection in the animal model.


RESULTS
The level of specific anti-KGPcd sIgA in saliva of the experimental group was significantly higher than that of control group. HE staining showed that immunization with recombined plasmid pcDNA3.1+/kgpcd could protect or minimize tissue destruction caused by subsequent P. gingivalis challenge in the rat model.


CONCLUSIONS
The results indicate that pcDNA3.1+/kgpcd was a good candidate for anti-periodontitis gene vaccine and could provide protection against Porphyromonas gingivalis-caused periodontitis in rat lesion model.
Zhang
Feng-qiu
FQ
Department of Periodontology, Peking University School of Stomatology, Beijing 100081, China.
Yang
Lian-jia
LJ
Wu
Zhi-fen
ZF
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology / Q000627:therapeutic use*; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D010518:Periodontitis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019444:Vaccines, DNA / Q000276:immunology / Q000627:therapeutic use*
D001428:Bacterial Vaccines; D007071:Immunoglobulin A, Secretory; D019444:Vaccines, DNA