Role of dprA in transformation of Campylobacter jejuni.
The role of a dprA ortholog (Cj0634) in Campylobacter jejuni transformation was phenotypically assessed using two strains. C. jejuni strain 11168 was naturally competent for transformation by chromosomal DNA, while efficiency decreased 100-fold in a Cj0634::aphA mutant, whereas C. jejuni strain 480 was not naturally competent. C. jejuni strain 480 but not 11168 could be electro-transformed by shuttle plasmid pRY111, an effect completely abolished by Cj0634 interruption. Complementation of the Cj0634 mutation in C. jejuni strain 480 in trans with vectors containing the dprA homologs from C. jejuni, Helicobacter pylori, or Haemophilus influenzae, completely (for Cj0634) or partially (H. pylori>H. influenzae) restored electro-transformation. Thus, C. jejuni expresses a DprA ortholog that functionally most closely resembles that of H. pylori and is involved in DNA transformation.
Takata
Tohru
T
Department of Medicine, New York University School of Medicine, New York, NY, USA. takattol@cis.fukuoka-u.ac.jp
Ando
Takafumi
T
Israel
Dawn A
DA
Wassenaar
Trudy M
TM
Blaser
Martin J
MJ
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D016123:Campylobacter jejuni / Q000235:genetics* / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000235:genetics; D018274:Electroporation; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D010957:Plasmids; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; C093815:DprA protein, bacteria; D008565:Membrane Proteins