Effects of diazoxide treatments on electrophysiologyic properties in guinea pig papillary muscles undergoing ischemia/reperfusion.
The effects of diazoxide treatments on electrophysiologyic properties in guinea pig papillary muscles undergoing ischemia/reperfusion was studied using intracellular microelectrode technique. Twenty-four guinea pigs were randomly divided into three groups (n=8 in each group). In control group, St. Thomas solution was given. In experimental group, St. Thomas solution with diazoxide (100 mol/L) was given. In pretreatment group, the muscle was treated with diazoxide 20 min before arrested with St. Thomas cardioplegia. The results showed that the APD50 and APD90 in experimental and pretreatment groups were significantly shorter after 5 and 10 min reperfusion (P <0.01, P<0.05), but longer after 30 min reperfusion (P<0.01, P<0.05) than in control group. In experimental and pretreatment groups, APA, OS, Vmax recovered more quickly than those in control group. The time to re-systole after reperfusion in control group was longer than that in experimental and pretreatment groups. There was no significant difference in RP among three groups. The time of arrest in pretreatment group was longer than that in experimental and pretreatment group (P<0.05). This study indicates that protective effects of St. Thomas solution with diazoxide is better than that of pretreatment with diazoxide or St. Thomas solution alone.
Yang
Yunhai
Y
Department of Cardiovascular Surgery, Union, Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Han
Zhaomin
Z
Hu
Zhiwei
Z
Zhang
Kailun
K
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002314:Cardioplegic Solutions; D003981:Diazoxide / Q000494:pharmacology*; D004594:Electrophysiology; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D008297:Male; D008839:Microelectrodes; D009200:Myocardial Contraction; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology* / Q000517:prevention & control; D010210:Papillary Muscles / Q000503:physiopathology*; D015221:Potassium Channels / Q000819:agonists* / Q000378:metabolism; D011897:Random Allocation
D002314:Cardioplegic Solutions; D015221:Potassium Channels; D000255:Adenosine Triphosphate; D003981:Diazoxide