[Determination gentiopicrin in biyanqingdu granulae by SPE-HPLC].
OBJECTIVE
The purpose was to develop SPE-HPLC method for the assay of gentiopicrin in Biyanqingdu Granulae.


METHODS
Separation was performed on a hypersil ODS(2) column (250 mm x 4.6 mm, 5 microm). The mobile phase consisted of methanol: water (30:70, v/v). The flow rate was 1 ml/min. The UV detect wavelength was set at 275nm.


RESULTS
Gentiopicrin had a good linear relation in the range of 0. 02235 - 0. 1676 mg/ml, the average recovery was 98.1% with RSD 1.7%.


CONCLUSIONS
The method is simple, sensitive and rapid and it can be applied to determine the content of Biyanqingdu Granulae.
Huang
Zhihai
Z
Guangdong Province Hospital of Troditional Chinese Medicine, Guangzhou.
Lin
Shengwen
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005960:Glucosides