title: Isolation and antifungal activity of 4-phenyl-3-butenoic acid from Streptomyces koyangensis strain VK-A60.
abstract: An antifungal compound was isolated from the culture broth of Streptomyces koyangensis strain VK-A60 using various chromatographic procedures. On the basis of the high-resolution EI-mass and 1H and 13C NMR data, the compound was identified as 4-phenyl-3-butenoic acid. Colletotrichum orbiculare, Magnaporthe grisea, and Pythium ultimum were most sensitive to 4-phenyl-3-butenoic acid. Strong inhibitory effects of 4-phenyl-3-butenoic acid also were found against Pectobacterium carotovorum subsp. carotovorum and Ralstonia solanacearum. 4-Phenyl-3-butenoic acid effectively suppressed the development of M. grisea on rice leaves at the concentration of more than 10 microg/mL, and the protective activity was in general similar to that of the commercial fungicide tricyclazole. Treatment with 100 microg/mL of 4-phenyl-3-butenoic acid also effectively inhibited the anthracnose development on cucumber plants, although its in vivo efficacy was somewhat less effective than that of the commercial fungicide chlorothalonil.
authors.affiliation: Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University, Seoul 136-713, Korea.
authors.forename: Jee Yeon
authors.initials: JY
authors.lastname: Lee
authors.forename: Jung Yeop
authors.initials: JY
authors.lastname: Lee
authors.forename: Surk Sik
authors.initials: SS
authors.lastname: Moon
authors.forename: Byung Kook
authors.initials: BK
authors.lastname: Hwang
mesh_terms: D017077:Culture Media, Conditioned / Q000737:chemistry; D005229:Fatty Acids, Monounsaturated / Q000302:isolation & purification* / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects; D005659:Fungicides, Industrial / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D005229:Fatty Acids, Monounsaturated; D005659:Fungicides, Industrial; C064180:4-phenyl-3-butenoic acid