title: Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas.
abstract: The complex interactions between cancer and host cells are far from being fully elucidated. Assessment of Th1/Th2/Th3/Tr1 balance is an interesting approach to explain immunological disturbances in lymphomas. The aim of our study was to assess mRNA for pro- and anti-inflammatory cytokines in T-cells in 20 children with Hodgkin- and non-Hodgkin lymphomas. CD4+ and CD8+ cells were isolated from whole peripheral blood and four different cytokine mRNA levels (IFN-gamma, IL-10, IL-4, TGF-beta) were determined by real-time PCR technique. Comparing to the control group, we found lower expression of mRNA for IFN-gamma in CD4+ cells at the time of lymphoma diagnosis. It may be one of the pathogenetic mechanisms of impaired immunity in these patients.
authors.affiliation: Department of Pediatric Hematology and Oncology, Medical University, Bialystok, Poland. vlodek@amb.edu.pl
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Luczyński
authors.forename: Oksana
authors.initials: O
authors.lastname: Kovalchuk
authors.forename: Maryna
authors.initials: M
authors.lastname: Krawczuk-Rybak
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Mitura-Lesiuk
authors.forename: Iwona
authors.initials: I
authors.lastname: Malinowska
authors.forename: Lech
authors.initials: L
authors.lastname: Chyczewski
authors.forename: Jerzy
authors.initials: J
authors.lastname: Kowalczyk
authors.forename: Michał
authors.initials: M
authors.lastname: Matysiak
mesh_terms: D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008223:Lymphoma / Q000175:diagnosis* / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D007371:Interferon-gamma