title: Adhesive resin and the hydrophilic monomer HEMA induce VEGF expression on dental pulp cells and macrophages.
abstract: Vascular endothelial growth factor (VEGF) plays an important role in angiogenesis by inducing endothelial cell proliferation, migration and survival. Direct pulp capping with an adhesive resin system was shown to induce local increase in blood vessel density and lack of dentin bridging. However, the mechanisms involved in the increase in blood vessel density observed near the pulp exposures capped with an adhesive resin are largely unknown. OBJECTIVES.: To investigate the effect of an adhesive resin or one of its hydrophilic monomers (HEMA), in the expression of VEGF by pulp cells. METHODS.: Mouse odontoblast-like cells (MDPC-23), undifferentiated pulp cells (OD-21), gingival fibroblasts, and macrophages were exposed to SingleBond (3M) or to 0-1000nM HEMA. VEGF expression was evaluated by ELISA and semi-quantitative RT-PCR. RESULTS AND SIGNIFICANCE.: VEGF expression was upregulated in MDPC-23 cells exposed to HEMA (p<0.001) or to SingleBond (p<0.018), and in macrophages exposed to HEMA (p<0.001) or SingleBond (p=0.001). In contrast, VEGF expression remained unchanged in undifferentiated pulp cells (OD-21), or fibroblasts exposed to either HEMA or Single Bond (p>0.05). Treatment with SingleBond or HEMA did not affect VEGF expression at the mRNA level of any cell type evaluated here, suggesting that the induction of VEGF expression in these cells is regulated primarily at the post-transcriptional level. These findings suggest that VEGF is involved in the regulation of pulp neovascularization observed in response to the application of adhesive resins at site of pulp exposure.
authors.affiliation: Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 N. University, Rm. 2309, Ann Arbor, MI 48109-1078, USA.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Mantellini
authors.forename: Tatiana
authors.initials: T
authors.lastname: Botero
authors.forename: Peter
authors.initials: P
authors.lastname: Yaman
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Dennison
authors.forename: Carl T
authors.initials: CT
authors.lastname: Hanks
authors.forename: Jacques E
authors.initials: JE
authors.lastname: Nör
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000633:toxicity; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003785:Dental Pulp Capping / Q000009:adverse effects; D003789:Dental Pulp Exposure / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008689:Methacrylates / Q000633:toxicity*; D051379:Mice; D009389:Neovascularization, Pathologic / Q000209:etiology; D009804:Odontoblasts / Q000187:drug effects* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D019279:Resin Cements / Q000633:toxicity*; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*
chemical_list: D008689:Methacrylates; D019279:Resin Cements; D042461:Vascular Endothelial Growth Factor A; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; C005044:hydroxyethyl methacrylate