[PCR-RFLP for Campylobacter jejuni subtyping].
Ten Campylobacter jejuni isolates, 8 identified as C. jejuni biotype II of Lior and 2 as C. jejuni biotipe I, were recovered from aborted pig fetuses. In order to discriminate among strains, restriction fragment length polymorphism (RFLP) using Ddel of polymerase chain reaction (PCR) products of flaA gen was used. C. jejuni biotype II strains could be diferenciated in 6 by PCR-RFLP, and one subtype was obtained from C. jejunibiotype I. Although there is great variability of molecular techniques applied to the Campylobacter epidemiological studies, PCR-RFLP demonstrated to be a simple and accessible technique to discriminate Campylobacter jejuni isolates.
Giacoboni
G
G
Laboratorio de Diagnóstico e Investigaciones Bacteriológicas, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118 CC296 (BI 1900AVW) La Plata, Argentina. giacoboni@fcv.unlp.edu.ar
Echeverría
M G
MG
Perfumo
C
C
D000034:Abortion, Veterinary / Q000209:etiology / Q000382:microbiology; D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D034421:Sus scrofa; D013553:Swine Diseases / Q000382:microbiology
D004269:DNA, Bacterial; C027908:endodeoxyribonuclease DdeI; D015252:Deoxyribonucleases, Type II Site-Specific