Characterization of genes for novel thaumatin-like proteins in Cryptomeria japonica.
Thaumatin-like proteins (TLPs) are induced by a variety of phytopathogens in many plants and several TLPs are allergenic. Previously, we isolated three TLP-encoding cDNAs (Cry j 3.1, Cry j 3.2 and Cry j 3.3) from a cDNA library derived from the pollen of Cryptomeria japonica D. Don. Here, we describe three new TLP cDNAs (Cry j 3.4, Cry j 3.5 and Cry j 3.6). We compared the sequences, the genetic map location and the expression patterns of the Cry j 3 genes. The amino acid sequence predicted from Cry j 3.5 exhibits only limited similarity to those predicted from the other Cry j 3 genes. Linkage analysis showed that the Cry j 3.1 to Cry j 3.4 genes are located in the same linkage group, but Cry j 3.5 is located in a different group. Organ-specificity and induction by stresses and plant hormones differed among the Cry j 3 mRNAs. In pollen grains, the Cry j 3.5 mRNA expression level was higher than that of the other Cry j 3 genes. Exposure to UV-B and salt stress induced expression of Cry j 3.1. The ethylene-releasing compound ethephon strongly induced expression of Cry j 3.4. Salt stress and salicylic acid also induced expression of Cry j 3.4. Abscisic acid weakly induced expression of Cry j 3.5. Arachidonic acid strongly induced expression of Cry j 3.4 and Cry j 3.6, and weakly induced that of Cry j 3.3, whereas expression of Cry j 3.1 and Cry j 3.5 was unaffected. These results suggest that the roles of TLPs and the cascades that regulate their expression differ among the members of the TLP family in C. japonica.
Futamura
Norihiro
N
Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, Ibaraki, Japan. futa@ffpri.affrc.go.jp
Tani
Naoki
N
Tsumura
Yoshihiko
Y
Nakajima
Nobuyoshi
N
Sakaguchi
Masahiko
M
Shinohara
Kenji
K
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000235:genetics; D016718:Arachidonic Acid / Q000494:pharmacology; D002874:Chromosome Mapping; D032485:Cryptomeria / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D005786:Gene Expression Regulation; D008040:Genetic Linkage; D018745:Genome, Plant; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D011058:Pollen / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D014466:Ultraviolet Rays
D052179:Antigens, Plant; C518869:Cry j 3 allergen, Cryptomeria japonica; D018076:DNA, Complementary; D009943:Organophosphorus Compounds; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D016718:Arachidonic Acid; D012965:Sodium Chloride; C003427:thaumatin protein, plant; D000040:Abscisic Acid; C005073:ethephon