title: Structural characterization of the proximal and distal histidine environment of cytoglobin and neuroglobin.
abstract: Cytoglobin (Cgb) and neuroglobin (Ngb) are the first examples of hexacoordinated globins from humans and other vertebrates in which a histidine (His) residue at the sixth position of the heme iron is an endogenous ligand in both the ferric and ferrous forms. Static and time-resolved resonance Raman and FT-IR spectroscopic techniques were applied in examining the structures in the heme environment of these globins. Picosecond time-resolved resonance Raman (ps-TR3) spectroscopy of transient five-coordinate heme species produced by the photolysis of carbon monoxide (CO) adducts of Cgb and Ngb showed Fe-His stretching (nu(Fe-His)) bands at 229 and 221 cm(-1), respectively. No time-dependent shift in the nu(Fe-His) band of Cgb and Ngb was detected in the 20-1000 ps time domain, in contrast to the case of myoglobin (Mb). These spectroscopic data, combined with previously reported crystallographic data, suggest that the structure of the heme pocket in Cgb and Ngb is altered upon CO binding in a manner different from that of Mb and that the scales of the structural alteration are different for Cgb and Ngb. The structural property of the heme distal side of the ligand-bound forms was investigated by observing the sets of (nu(Fe-CO), nu(C-O), delta(Fe-C-O)) and (nu(Fe-NO), nu(N-O), delta(Fe-N-O)) for the CO and nitric oxide (NO) complexes of Cgb and Ngb. A comparison of the spectra of some distal mutants of Cgb (H81A, H81V, R84A, R84K, and R84T) and Ngb (H64A, H64V, K67A, K67R, and K67T) showed that the CO adducts of Cgb and Ngb contained three conformers and that the distal His (His81 in Cgb and His64 in Ngb) mainly contributes to the interconversion of the conformers. These structural characteristics of Cgb and Ngb are discussed in relation to their ligand binding and physiological properties.
authors.affiliation: Department of Life Science, Graduate School of Science, Himeji Institute of Technology/University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako, Hyogo 678-1297, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Sawai
authors.forename: Masatomo
authors.initials: M
authors.lastname: Makino
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Mizutani
authors.forename: Takehiro
authors.initials: T
authors.lastname: Ohta
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugimoto
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Uno
authors.forename: Norifumi
authors.initials: N
authors.lastname: Kawada
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yoshizato
authors.forename: Teizo
authors.initials: T
authors.lastname: Kitagawa
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Shiro
mesh_terms: D002248:Carbon Monoxide / Q000737:chemistry; D003461:Crystallography; D000078842:Cytoglobin; D005914:Globins / Q000737:chemistry*; D006418:Heme / Q000737:chemistry; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D007501:Iron / Q000737:chemistry; D008024:Ligands; D008027:Light; D008956:Models, Chemical; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D000078787:Neuroglobin; D009569:Nitric Oxide / Q000737:chemistry; D010782:Photolysis; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: C000627265:CYGB protein, human; D000078842:Cytoglobin; D008024:Ligands; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D011994:Recombinant Proteins; D009569:Nitric Oxide; D006418:Heme; D006639:Histidine; D002248:Carbon Monoxide; D005914:Globins; D007501:Iron