title: [Determination of fumonisin biosynthesis gene and toxicity of Fusarium moniliforme strains isolated in China].
abstract: OBJECTIVE
  To study the relationship between fumonisin biosynthesis gene and toxicity of Fusarium moniliforme isolated in China.
  
  
  METHODS
  The toxigenic gene of 29 Fusarium moniliforme isolated from different provinces and varied food samples were determined. Eighteen fum5-positive strains were selected for biosyhesizing fumonisin and determined by high performance liquid chromatography (HPLC).
  
  
  RESULTS
  Twenty-six isolates were identified as fum5 gene positive strains. And all of these strains produced FB(1), FB(2) and FB(3). The amount of FB(1), FB(2) and FB(3) was ranging from 0.41-140.20 mg/kg, 0.06-14.30 mg/kg to 0.30-58.00 mg/kg, except one strain produced a lower level of FB(1) only. It wight be the first report showing a high level fumonisin-producing strain isolated from the sesame sample and identified in the world. The amount of FB(1), FB(2) and FB(3) produced by the isolate was 128.84 mg/kg, 11.80 mg/kg and 14.88 mg/kg.
  
  
  CONCLUSIONS
  It should have a close relationship between fumonisins biosynthesis gene and toxicity of Fusarium moniliforme isolated in China. The study demonstrated that strain of Fusarium moniliforme might contaminate the sesame sample and produce a high level of fumonisins.
authors.affiliation: National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Xiu-mei
authors.initials: XM
authors.lastname: Liu
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Mao-feng
authors.initials: MF
authors.lastname: Qiu
authors.forename: Xiu-fang
authors.initials: XF
authors.lastname: Li
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D037341:Fumonisins / Q000032:analysis / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005670:Fusarium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D031685:Sesamum / Q000382:microbiology
chemical_list: D037341:Fumonisins; D005656:Fungal Proteins