Cytotoxic and genotoxic effects of the azo-dye p-dimethylaminoazobenzene in mice: a time-course study.
The cytotoxic and genotoxic effects of chronic feeding of the azo-dye p-dimethylaminoazobenzene (p-DAB) during 7, 15, 30, 60, 90 and 120 days have been assessed in mice. The endpoints used for genotoxic analysis were chromosome aberrations (CA), micronuclei (MN) and mitotic index (MI) in bone-marrow cells, and sperm-head abnormality (SHA) in male gonads. The activities of marker enzymes for toxicity, such as glutamate oxalo-acetate transaminase (GOT), glutamate pyruvate transaminase (GPT), acid phosphatase (ACP) and alkaline phosphatase (ALKP) were also assayed periodically, as was lipid peroxidation (LPO). Chronic feeding of p-DAB produced increased numbers of chromosome aberrations, nuclear anomalies and sperm-head abnormalities, as compared with normal untreated controls, generally in a time-dependent manner until 60 days, after which the anomalies persisted, but rather erratically. However, although there was some noticeable modulation in enzyme activities in the corresponding p-DAB-fed mice as well, these were not strictly time-dependent.
Biswas
Surjyo Jyoti
SJ
Cytogenetics Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India.
Khuda-Bukhsh
Anisur Rahman
AR
D000284:Administration, Oral; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002869:Chromosome Aberrations / Q000139:chemically induced*; D051379:Mice; D015162:Micronucleus Tests; D008940:Mitotic Index; D013997:Time Factors; D004124:p-Dimethylaminoazobenzene / Q000008:administration & dosage / Q000633:toxicity*
D002273:Carcinogens; D004124:p-Dimethylaminoazobenzene