Quantification of several monohydroxylated metabolites of polycyclic aromatic hydrocarbons in urine by high-performance liquid chromatography with fluorescence detection.
A high-performance liquid chromatographic method with fluorescence detection has been developed to determine the urinary polycyclic aromatic hydrocarbon metabolites 2-hydroxynaphthalene, 2-hydroxyfluorene, 9-hydroxyphenanthrene, 1-hydroxypyrene and 3-hydroxybenz[a]pyrene. Solid phase extraction (SPE) was used to clean up the samples, and washing with 30% methanol was found to be the best way to remove interferences in the matrix. The method detection limits ranged from 0.044 microg/L for 1-hydroxypyrene to 1.615 microg/L for 3-hydroxybenz[a]pyrene, and the recoveries ranged between 40% for 3-hydroxybenz[a]pyrene and 99% for 2-hydroxynaphthalene. The within-day relative standard deviation was lowest for 2-hydroxynaphthalene at 0.67% and went up to 2.42% for 3-hydroxybenz[a]pyrene, and the between-day relative standard deviation ranged from 3.84% for 9-hydroxyphenanthrene to 10.42% for 2-hydroxyfluorene. The correlation coefficients were between 0.9962 and 0.9998. The developed method was successfully used to analyze samples from student volunteers in a high school.
Wang
Yu
Y
Guangdong Key Laboratory of Environment Protection & Resources Utilization, State Key Laboratory of Organic Geochemistry, Guangzhuo Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, Guangdong, 510640, P.R. China.
Zhang
Wenbing
W
Dong
Yulian
Y
Fan
Ruifang
R
Sheng
Guoying
G
Fu
Jiamo
J
D000465:Algorithms; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants / Q000652:urine*; D006801:Humans; D011084:Polycyclic Aromatic Hydrocarbons / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D016482:Urinalysis / Q000379:methods*
D004785:Environmental Pollutants; D011084:Polycyclic Aromatic Hydrocarbons