Linkage map of protein-protein interactions of Porcine teschovirus.
A yeast two-hybrid study was conducted to catalogue the protein-protein interactions of the Porcine teschovirus non-structural proteins. Five homodimer, three reciprocal heterodimer and four unidirectional heterodimer interactions were observed. While several interactions are similar to those described in previous studies using enteroviruses, such as homo- and heterodimeric interactions of the 2B, 3CD and 3D proteins, several were not found previously. Among these is the binding of the leader protein L to the proteinases 3C and 3CD. Unlike the poliovirus 3C, the teschovirus 3C proteinase dimerizes and interacts with 2BC, 3CD and 3D. The strongest interactions were observed for L-3C, L-3CD and 3C-3CD.
Zell
Roland
R
Institute for Virology and Antiviral Therapy, Hans-Knöll-Str. 2, D-07745 Jena, Germany.
Seitz
Simone
S
Institute for Virology and Antiviral Therapy, Hans-Knöll-Str. 2, D-07745 Jena, Germany.
Henke
Andreas
A
Institute for Virology and Antiviral Therapy, Hans-Knöll-Str. 2, D-07745 Jena, Germany.
Munder
Thomas
T
Leibniz Institute for Natural Products Research and Infection Biology, Hans Knöll Institute, Beutenbergstr. 11a, D-07745 Jena, Germany.
Wutzler
Peter
P
Institute for Virology and Antiviral Therapy, Hans-Knöll-Str. 2, D-07745 Jena, Germany.
D000086042:3C Viral Proteases; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D008040:Genetic Linkage; D010849:Picornaviridae / Q000235:genetics* / Q000502:physiology; D020798:Two-Hybrid System Techniques; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins
D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases