Dual-probe assay for detection of lamivudine-resistance hepatitis B virus by real-time PCR.
A rapid and accurate minor groove binder (MGB) real-time PCR is described for detection lamivudine resistance mutations in hepatitis B virus. The real-time PCR was compared with direct Sanger sequencing in 53 clinical patients samples, the results of the real-time PCR correlate with the nucleotide sequence and the assay has the advantage of detecting a mixture of quasi-species with higher sensitivity than sequencing. As a simple, easy, rapid, accurate and high throughout method, MGB real-time PCR assay should be useful for detecting lamivudine resistance variants during lamivudine therapy in clinical settings.
Geng
Haifeng
H
Nucleic acid diagnosis Lab, Shanghai Fudan-Yueda Bio-Tech Co. Ltd, Juli Road No.1., Shanghai Zhangjiang Hi-Tec. District, Shanghai 200240, China. creageng@yahoo.com.cn
Hua
Bing
B
Wang
Hua
H
Cao
Yanhong
Y
Sun
Yun
Y
Yu
Airong
A
D000998:Antiviral Agents / Q000494:pharmacology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D024882:Drug Resistance, Viral / Q000235:genetics*; D006509:Hepatitis B / Q000821:virology; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D019259:Lamivudine / Q000494:pharmacology*; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D000998:Antiviral Agents; D004279:DNA, Viral; D019259:Lamivudine