Isoquercitrin from Argemone platyceras inhibits carbachol and leukotriene D4-induced contraction in guinea-pig airways.
Argemone platyceras is used in Mexico as a remedy for cough, bronchitis and pneumonia. The present study was performed to investigate the pharmacological anti-asthmatic properties of Argemone platyceras on airways and to identify its active principles. Methanol extracts of leaves and flowers, subsequent organic and aqueous extraction phases, and silica gel chromatography fractions were assayed on the carbachol-induced response, and/or on ovalbumin antigenic challenge, and on leukotriene D(4)-induced response of tracheae from sensitized and non-sensitized guinea-pigs. Methanol extracts, ethyl-acetate phase, and its fractions 6 and 7 inhibited the carbachol-induced contractile response. Isoquercitrin and rutin were the main compounds found in fractions 6 and 7 respectively. Isoquercitrin (fraction 6) abolished the response to ovalbumin, and decreased the contractile response to leukotriene D(4). Because of its effect on carbachol-induced contractile response, on the late-phase response to ovalbumin, and on leukotriene D(4)-induced contractile response, isoquercitrin might be highly useful in treatment of asthma.
Fernandez
Jacquelina
J
Unidad de Investigación Médica en Farmacología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico.
Reyes
Ricardo
R
Ponce
Hector
H
Oropeza
Martha
M
Vancalsteren
Marie-Rose
MR
Jankowski
Christopher
C
Campos
Maria G
MG
D000085:Acetates / Q000737:chemistry; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D031674:Argemone / Q000737:chemistry*; D001249:Asthma / Q000276:immunology / Q000517:prevention & control; D016084:Bronchoconstriction / Q000187:drug effects*; D002217:Carbachol / Q000494:pharmacology; D005591:Chemical Fractionation; D004305:Dose-Response Relationship, Drug; D035264:Flowers / Q000737:chemistry; D006168:Guinea Pigs; D007213:Indomethacin / Q000494:pharmacology; D017998:Leukotriene D4 / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D009119:Muscle Contraction / Q000187:drug effects*; D010047:Ovalbumin / Q000276:immunology; D008517:Phytotherapy; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012431:Rutin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013997:Time Factors; D014132:Trachea / Q000187:drug effects / Q000503:physiopathology
D000085:Acetates; D018927:Anti-Asthmatic Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; C016527:isoquercitrin; D012431:Rutin; D017998:Leukotriene D4; C007650:ethyl acetate; D002217:Carbachol; D010047:Ovalbumin; D011794:Quercetin; D007213:Indomethacin; D000432:Methanol