[The variations of p38 MAPK activity on lipopolysaccharide-induced inflammation of nasal epithelial cells and its significance in vitro].
OBJECTIVE
To study the variations of p38 mitogen-activated protein kinase (p38MAPK) activity on lipopolysaccharide (LPS)-induced inflammation of nasal epithelial cells and its significance in vitro.


METHODS
Epithelial cells from normal nasal mucosa were separated and cultured without serum. The epithelial cells were stimulated by LPS in different concentration; Western immunoblotting was used to detect the variations of p38MAPK activity and the inhibited effect of SB203580 which is a specific inhibitor of p38MAPK.


RESULTS
The activation of p38MAPK stimulated by LPS from 5-30 min and 0.01-1.0 mg/L was enhanced. SB203580 at the dose of 1.0-5.0 tmol/L has the highest inhibition to the activation of p38MAPK. SB203580 can inhibited the activation of p38MAPK before 20 min stimulated by LPS, which has little inhibition after stimulated by LPS.


CONCLUSIONS
LPS induced inflammatory response of the epithelial cells in nasal mucosa can cause the enhancement of p38MAPK activity at "positive dependence" pattern whiting 30 min and 1 mg/L. The inhibition of SB203580 on the activation of p38MAPK was enhanced at "positive dependence" pattern with 5 micromol/L, which was effective only before the stimulation of LPS.
Li
Tongli
T
Department of Otorhinolaryngology, Shanxi Province People Hospital, Taiyuan, 030012, China. t.l.li@163.com
Li
Yuan
Y
Zhang
Gehua
G
D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D007249:Inflammation; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009297:Nasal Mucosa / Q000166:cytology; D011725:Pyridines / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D004791:Enzyme Inhibitors; D007093:Imidazoles; D008070:Lipopolysaccharides; D011725:Pyridines; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580