Moment analysis for kinetics of gene silencing by RNA interference.
RNA interference (RNAi) was quantitatively evaluated from a kinetic viewpoint. A simple kinetic evaluation based on moment analysis was proposed, assuming suppression and recovery phases of gene expression. We defined the area under the curve of the inhibitory effect (AUC(IE)) as an index of the total intensity of RNAi and the mean response time of the inhibitory effect (MRT(IE)) as an index of its duration. The proposed kinetic analysis helps to understand the RNAi effect in a quantitative and time-dependent manner, which will be beneficial for designing RNAi-based gene silencing for both experimental and therapeutic purposes.
Takahashi
Yuki
Y
Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
Yamaoka
Kiyoshi
K
Nishikawa
Makiya
M
Takakura
Yoshinobu
Y
D000818:Animals; D019540:Area Under Curve; D045744:Cell Line, Tumor; D007700:Kinetics; D049409:Luciferases, Firefly / Q000235:genetics / Q000378:metabolism; D049410:Luciferases, Renilla / Q000235:genetics / Q000378:metabolism; D051379:Mice; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D034741:RNA, Small Interfering; D049410:Luciferases, Renilla; D049409:Luciferases, Firefly