title: Nanoliquid chromatography-mass spectrometry of oligosaccharides employing graphitized carbon chromatography on microchip with a high-accuracy mass analyzer.
abstract: The nanoLC separations of oligosaccharides using microchip-based columns are described. Mixtures of alditols from mucins and human milk are separated on graphitized carbon. The nanoLC-MS device showed high mass accuracy for the oligosaccharides ranging between 1 and 6 ppm on routine analyses. The high mass accuracy readily allowed identification of oligosaccharide peaks and the determination of their compositions. High retention time reproducibility was exhibited by the microchip LC. Little variation was observed for standard sample either alone or in a complex heterogeneous mixture. The nanoLC-MS exhibits excellent capabilities in profiling mixtures of oligosaccharides.
authors.affiliation: Department of Chemistry, University of California, Davis, CA 95616, USA.
authors.forename: Milady
authors.initials: M
authors.lastname: Niñonuevo
authors.forename: Hyunjoo
authors.initials: H
authors.lastname: An
authors.forename: Hongfeng
authors.initials: H
authors.lastname: Yin
authors.forename: Kevin
authors.initials: K
authors.lastname: Killeen
authors.forename: Rudi
authors.initials: R
authors.lastname: Grimm
authors.forename: Robert
authors.initials: R
authors.lastname: Ward
authors.forename: Bruce
authors.initials: B
authors.lastname: German
authors.forename: Carlito
authors.initials: C
authors.lastname: Lebrilla
mesh_terms: D002244:Carbon / Q000737:chemistry; D002853:Chromatography, Liquid / Q000295:instrumentation*; D006801:Humans; D013058:Mass Spectrometry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008895:Milk, Human / Q000737:chemistry; D009077:Mucins / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation*; D009844:Oligosaccharides / Q000032:analysis*
chemical_list: D009077:Mucins; D009844:Oligosaccharides; D002244:Carbon