title: [Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province].
abstract: OBJECTIVE
  To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene.
  
  
  METHODS
  PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program.
  
  
  RESULTS
  Newly isolated Japanese encephalitis viruses belonged to genotype III, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA14-14-2, but had some difference in some domains.
  
  
  CONCLUSIONS
  Newly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype III. E protein of the isolates showed some differences as compared with vaccine strains.
authors.affiliation: State Key Laboratory for Molecular Virology, Fujian Center for Disease Control and Prevention, Fuzhou 350001, China.
authors.forename: Duan
authors.initials: D
authors.lastname: Chen
authors.forename: Huan-yu
authors.initials: HY
authors.lastname: Wang
authors.forename: Shi-hong
authors.initials: SH
authors.lastname: Fu
authors.forename: Hong
authors.initials: H
authors.lastname: Song
authors.forename: Juan
authors.initials: J
authors.lastname: Deng
authors.forename: Yi-liang
authors.initials: YL
authors.lastname: Yang
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004672:Encephalitis, Japanese / Q000821:virology*; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral; D014759:Viral Envelope Proteins; C074932:prM protein, Flavivirus