Biological characteristics of human bone marrow mesenchymal stem cell cultured in vitro.
Some biological characteristics of human bone marrow mesenchymal stem cells (MSCs) cultured in vitro were observed. hMSCs were isolated from bone marrow and purified by density gradient centrifugation method, and then cultured in vitro. The proliferation and growth characteristics of hMSCs were observed in primary and passage culture. MSCs of passage 3 were examined for the purify by positive rate of CD29 and CD44 through flow cytometry. Human bone marrow MSCs showed active proliferation capacity in vitro. The purify of MSCs separated by our method was higher than 90%. It was concluded that hMSCs have been successfully cultured and expanded effectively. It provided a foundation for further investigation and application of MSCs.
Fa
Xian'en
X
Department of Cardiac' Surgery, Union Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan 430022, China.
Lixia
Wang
W
Hou
Jianfeng
J
Zhang
Ruicheng
R
Wang
Haiyong
H
Yang
Chenyuan
C
D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D019012:Integrin beta1 / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000166:cytology*
D018960:Hyaluronan Receptors; D019012:Integrin beta1