Molecular dynamics imaging in micropatterned living cells.
Micropatterning approaches using self-assembled monolayers of alkyl thiols on gold are not optimal for important imaging modalities in cell biology because of absorption of light and scattering of electrons by the gold layer. We report here an anisotropic solid microetching (ASOMIC) procedure that overcomes these limitations. The method allows molecular dynamics imaging by wide-field and total internal reflection fluorescence (TIRF) microscopy of living mammalian cells and correlative platinum replica electron microscopy.
Kandere-Grzybowska
Kristiana
K
Department of Cell and Molecular Biology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, Illinois 60611-3008, USA.
Campbell
Christopher
C
Komarova
Yulia
Y
Grzybowski
Bartosz A
BA
Borisy
Gary G
GG
D000818:Animals; D003599:Cytoskeleton / Q000648:ultrastructure*; D006046:Gold / Q000737:chemistry*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010984:Platinum / Q000737:chemistry*; D016628:Replica Techniques; D013438:Sulfhydryl Compounds / Q000737:chemistry
D013438:Sulfhydryl Compounds; D010984:Platinum; D006046:Gold