Phenotypic, functional, and quantitative characterization of canine peripheral blood monocyte-derived macrophages.
The yield as well as phenotypic and functional parameters of canine peripheral blood monocyte-derived macrophages were analyzed. The cells that remained adherent to Teflon after 10 days of culture had high phagocytic activity when inoculated with Leishmania chagasi. Flow cytometric analysis demonstrated that more than 80% of cultured cells were positive for the monocyte/macrophage marker CD14.
Bueno
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Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Mello
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MN
Menezes
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CA
Dutra
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WO
Santos
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RL
D000818:Animals; D015415:Biomarkers; D004285:Dogs; D005260:Female; D005434:Flow Cytometry; D007891:Leishmania / Q000502:physiology*; D018950:Lipopolysaccharide Receptors; D008262:Macrophage Activation; D008264:Macrophages / Q000469:parasitology* / Q000502:physiology; D008297:Male; D009000:Monocytes / Q000469:parasitology / Q000502:physiology; D010587:Phagocytosis; D010641:Phenotype; D013997:Time Factors
D015415:Biomarkers; D018950:Lipopolysaccharide Receptors