title: Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit.
abstract: The Lightcycler Staphylococcus kit is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers.
authors.affiliation: Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands.
authors.forename: Roos S R
authors.initials: RS
authors.lastname: Choudhurry
authors.forename: Damian C
authors.initials: DC
authors.lastname: Melles
authors.forename: Kimberly
authors.initials: K
authors.lastname: Eadie
authors.forename: Margreet
authors.initials: M
authors.lastname: Vos
authors.forename: Heiman F L
authors.initials: HF
authors.lastname: Wertheim
authors.forename: Henry A
authors.initials: HA
authors.lastname: Verbrugh
authors.forename: Alex
authors.initials: A
authors.lastname: van Belkum
authors.forename: Willem B
authors.initials: WB
authors.lastname: van Leeuwen
mesh_terms: D002353:Carrier State / Q000382:microbiology*; D006801:Humans; D009296:Nasal Cavity / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D011933:Reagent Kits, Diagnostic