RTN4-C gene expression in hepatocellular carcinoma and its influence on SMMC7721 cell growth and apoptosis.
RTN4-C gene is a member of RTNs. To investigate its expression in human hepatocellular carcinoma tissues and study its function on SMMC7721 cells, RT-PCR was conducted to detect its expressions in human hepatocellular carcinoma tissues. RTN4-C-pcDNA3. 1 plasmid was reconstructed and stably transfected into SMMC7721 cells by Lipofect AMINE. Growth curve of SMMC7721 measured by MTT and apoptosis indentified with acridine orange staining were examined to demonstrate the effect of RTN4-C on SMMC7721. Immunocytochemical analysis for mutant p53, c-Fos, Hsp70 proteins were conducted. The results showed that the transfected SMMC7721 cells grew more slowly than control and the average inhibition rate at the 1st, 2nd and 3rd day were 33.8% +/- 0.026, 56.2% +/- 0. 094, 34.8% +/- 0.077 respectively. In transfected SMMC7721 cell line, the apoptosis was strengthened,mutant p53 protein tranferred from nucleus to cytoplasm and c-Fos, Hsp70 proteins were decreased. Our data indicated RTN4-C gene was expressed differently in hepatocellular carcinoma and its paracancerous tissues. By tranferring mutant p53 protein from nucleus to cytoplasm and decreasing c-Fos, Hsp70 protein expression, RTN4-C inhibited SMMC7721 cells growth and promoted its apoptosis.
Chen
Yi-Cun
YC
The College of Life Science, Nanjing Normal University, Jiangsu Key Laboratory of Molecular and Medical Biotechnology, Nanjing 210097, China.
Lu
Dong-Dong
DD
Cao
Xiang-Rong
XR
Zhang
Xi-Ran
XR
D000328:Adult; D000368:Aged; D017209:Apoptosis; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D050505:Mutant Proteins / Q000378:metabolism; D009185:Myelin Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D000070798:Nogo Proteins; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D050505:Mutant Proteins; D009185:Myelin Proteins; D000070798:Nogo Proteins; D016760:Proto-Oncogene Proteins c-fos; C000603199:RTN4 protein, human; D016159:Tumor Suppressor Protein p53