Contribution of mutation at amino acid 45 of AcrR to acrB expression and ciprofloxacin resistance in clinical and veterinary Escherichia coli isolates.
Fluoroquinolone-resistant Escherichia coli isolates which overexpressed acrB and had a substitution at amino acid 45 of AcrR were complemented with wild-type acrR. Complementation led to increased sensitivity to ciprofloxacin and to ethidium bromide, suggesting that mutation at amino acid 45 of AcrR contributes to ciprofloxacin resistance.
Webber
Mark A
MA
Antimicrobial Agents Research Group, Division of Immunity and Infection, The University of Birmingham, Edgbaston, UK.
Talukder
Ashraf
A
Piddock
Laura J V
LJ
D019943:Amino Acid Substitution; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics*; D008826:Microbial Sensitivity Tests
D000890:Anti-Infective Agents; D029968:Escherichia coli Proteins; D002939:Ciprofloxacin