Quantitative determination of diterpenoid alkaloids in four species of Aconitum by HPLC.
A high performance liquid chromatography (HPLC) method has been developed for the determination of five principal alkaloids (benzoylmesaconine, mesaconitine, aconitine, hypaconitine and deoxyaconitine) found in four species of genus Aconitum. The five alkaloids were analyzed simultaneously with an XTerraRP18 column by gradient elution using 0.03 M ammonium hydrogen carbonate-acetonitrile as mobile phase. The recovery of the method was 94.6-101.9%, and all the alkaloids showed good linearity (gamma = 0.9999) in a relatively wide concentration range. The results indicated that contents of alkaloids in Aconitum varied significantly from species to species; hence quality control of Aconitum drugs is very necessary.
Wang
Zhaohong
Z
Forensic Science Institute of Public Security Ministry, Toxicology Analysis, Muxidi Nanli 17, Xicheng District, Beijing 100038, PR China. wwwzhaohong@hotmail.com
Wen
Jiao
J
Xing
Junbo
J
He
Ye
Y
D000157:Aconitine / Q000031:analogs & derivatives / Q000032:analysis; D031841:Aconitum / Q000737:chemistry* / Q000145:classification; D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004224:Diterpenes / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D018517:Plant Roots; D012044:Regression Analysis; D015203:Reproducibility of Results
D000470:Alkaloids; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; C057219:deoxyaconitine; C058309:hypaconitine; C019470:mesaconitine; C085283:benzoylmesaconine; D000157:Aconitine