Overexpression of LAMP3/TSC403/DC-LAMP promotes metastasis in uterine cervical cancer.
LAMP3 (DC-LAMP, TSC403, CD208) was originally isolated as a gene specifically expressed in lung tissues. LAMP3 is located on a chromosome 3q segment that is frequently amplified in some human cancers, including uterine cervical cancer. Because two other members of the LAMP family of lysosomal membrane glycoproteins, LAMP1 and LAMP2, were previously implicated in potentially modulating the interaction of vascular endothelial and cancer cells, we hypothesized that LAMP3 might also play an important part in metastasis. To clarify the metastatic potential of LAMP3 in cervical cancers, we transfected a LAMP3 expression vector into a human uterine cervical cancer cell line, TCS. In an in vitro invasion assay, the migration of LAMP3-overexpressing TCS cells was significantly higher than in control TCS cells. In an in vivo metastasis assay, distant metastasis was detected in 9 of 11 LAMP3-overexpressing TCS cell-injected mice and in only 1 of 11 control mice. Histologic study showed that LAMP3-overexpressing cells readily invaded into the lymph-vascular space. In clinical samples, quantitative real-time reverse transcription-PCR (RT-PCR) analyses showed that LAMP3 mRNA was significantly up-regulated in 47 of 47 (100%) cervical cancers and in 2 of 15 (13%) cervical intraepithelial neoplasias, compared with a low level of LAMP3 mRNA expressed in normal uterine cervixes. Interestingly, high LAMP3 expression was significantly correlated with the overall survival of patients with stage I/II cervical cancers. These findings indicate that LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer.
Kanao
Hiroyuki
H
Department of Obstetrics and Gynecology, Osaka University Faculty of Medicine, Osaka, Japan.
Enomoto
Takayuki
T
Kimura
Toshihiro
T
Fujita
Masami
M
Nakashima
Ryuichi
R
Ueda
Yutaka
Y
Ueno
Yuko
Y
Miyatake
Takashi
T
Yoshizaki
Tatsuo
T
Buzard
Gregory S
GS
Tanigami
Akira
A
Yoshino
Kiyoshi
K
Murata
Yuji
Y
D000818:Animals; D002465:Cell Movement / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D051907:Lysosomal Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics*; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D009857:Oncogenes / Q000235:genetics; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection; D014183:Transplantation, Heterologous; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D059666:Lysosomal-Associated Membrane Protein 3
D051907:Lysosomal Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C114233:LAMP3 protein, human; D059666:Lysosomal-Associated Membrane Protein 3