title: Synthetic vascular prosthesis impregnated with genetically modified bone marrow cells produced recombinant proteins.
abstract: The aim of this study is to develop an experimental model of small caliber expanded polytetrafluoroethylene (ePTFE) vascular prostheses that produce recombinant proteins by seeding genetically modified bone marrow mesenchymal stem cells (MSC). Beta-galactosidase (beta-gal) cDNA was transduced into rat MSC mediated by an adenovirus vector. The cells were impregnated into the ePTFE vascular prostheses measuring 2 mm in internal diameter and 90 microm in fibril length, followed by 48 h of incubation. The expressions of beta-gal were determined by X-gal staining. The luminal surface of the ePTFE vascular prostheses was covered with the MSC expressing beta-gal. Most of the gene-transduced MSC spread along the fibers forming colonies. These results suggest that small caliber vascular prostheses, in which the inner surface was seeded by genetically modified MSC, produced recombinant proteins. This may be a preliminary model to autocrine functioning vascular prostheses.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Osaka Medical College, Takatsuki, Japan. tho064@poh.osaka-med.ac.jp
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kanki-Horimoto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Horimoto
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Mieno
authors.forename: Kenji
authors.initials: K
authors.lastname: Kishida
authors.forename: Fusao
authors.initials: F
authors.lastname: Watanabe
authors.forename: Eisuke
authors.initials: E
authors.lastname: Furuya
authors.forename: Takahiro
authors.initials: T
authors.lastname: Katsumata
mesh_terms: D000256:Adenoviridae; D000818:Animals; D001807:Blood Vessel Prosthesis; D020099:Coated Materials, Biocompatible; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D011138:Polytetrafluoroethylene; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D020099:Coated Materials, Biocompatible; D011994:Recombinant Proteins; D011138:Polytetrafluoroethylene; D001616:beta-Galactosidase