Evaluating cutaneous photoaging by use of multiphoton fluorescence and second-harmonic generation microscopy.
The photoaging process of facial skin is investigated by use of multiphoton fluorescence and second-harmonic generation (SHG) microscopy. We obtain the autofluorescence (AF) and SHG images of the superficial dermis from the facial skin of three patients aged 20, 40, and 70 years. The results show that areas of AF increase with age, whereas areas of SHG decrease with age. The results are consistent with the histological findings in which collagen is progressively replaced by elastic fibers. The AF and SHG changes in photoaging are quantified by a SHG to autofluorescence aging index of dermis (SAAID). Our results suggest that SAAID can be a good indicator of the severity of photoaging.
Lin
Sung-Jan
SJ
Dermatology, Taipei Campus and Yun-Lin Branch, National Taiwan University Hospital, Taiwan, Taipei.
Wu
Ruei-
R
Tan
Hsin-Yuan
HY
Lo
Wen
W
Lin
Wei-Chou
WC
Young
Tai-Horng
TH
Hsu
Chih-Jung
CJ
Chen
Jau-Shiuh
JS
Jee
Shiou-Hwa
SH
Dong
Chen-Yuan
CY
D000328:Adult; D000368:Aged; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D015595:Skin Aging / Q000473:pathology* / Q000502:physiology*