[The preliminary study on the characteristics of mandible and condyle in dentin matrix protein-1 gene knockout mice].
OBJECTIVE
To study the characteristics of mandible and condyle in Dmp1 gene knockout mice, and to investigate the role of Dmp1 in the osteogenesis and mineralization of bone and cartilage.


METHODS
Dmp1-/-mice were executed at birth, 2 weeks, 2 months, 3 months and 5 months, and the mandible was taken out for physical, radiography, transmission electron microscopic, and histological examination. The difference between Dmp1 knockout mouse (ko) and wild type mouse (wt) in bone development, bone densitometry and histology were compared.


RESULTS
There were obvious changes in the mandible and condyle of Dmp1-/-mouse, such as incomplete ossification, low density, decreased volume and condyle cartilage degeneration.


CONCLUSIONS
Dmp1 is the key factor in the formation of growth plates and secondary ossification center, and plays an important role in the process of bone and cartilage formation and bone nodule remodeling. Dmp1 may be the candidate gene that controls the development of mandible and cartilage.
Li
Zu-bing
ZB
Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China. lizubing0827@163.com
Li
Zhi
Z
Shang
Zheng-jun
ZJ
D000818:Animals; D018488:Bone Demineralization, Pathologic / Q000235:genetics / Q000473:pathology*; D020219:Chondrogenesis / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D055786:Gene Knockout Techniques; D008334:Mandible / Q000473:pathology*; D008335:Mandibular Condyle / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010012:Osteogenesis / Q000235:genetics*
C513308:Dmp1 protein, mouse; D016326:Extracellular Matrix Proteins