Cnu, a novel oriC-binding protein of Escherichia coli.
We have found, using a newly developed genetic method, a protein (named Cnu, for oriC-binding nucleoid-associated) that binds to a specific 26-base-pair sequence (named cnb) in the origin of replication of Escherichia coli, oriC. Cnu is composed of 71 amino acids (8.4 kDa) and shows extensive amino acid identity to a group of proteins belonging to the Hha/YmoA family. Cnu was previously discovered as a protein that, like Hha, complexes with H-NS in vitro. Our in vivo and in vitro assays confirm the results and further suggest that the complex formation with H-NS is involved in Cnu/Hha binding to cnb. Unlike the hns mutants, elimination of either the cnu or hha gene did not disturb the growth rate, origin content, and synchrony of DNA replication initiation of the mutants compared to the wild-type cells. However, the cnu hha double mutant was moderately reduced in origin content. The Cnu/Hha complex with H-NS thus could play a role in optimal activity of oriC.
Kim
Myung Suk
MS
Department of Biology, School of Biological Sciences and Biotechnology, Chungnam National University, Taejon, 305-764 Korea.
Bae
Sung-Hun
SH
Yun
Sang Hoon
SH
Lee
Hee Jung
HJ
Ji
Sang Chun
SC
Lee
Ji Hyun
JH
Srivastava
Preeti
P
Lee
Seol-Hoon
SH
Chae
Huiseok
H
Lee
Younghoon
Y
Choi
Byong-Seok
BS
Chattoraj
Dhruba K
DK
Lim
Heon M
HM
D000595:Amino Acid Sequence; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D051738:Origin Recognition Complex; D010641:Phenotype; D011485:Protein Binding / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
C505237:Cnu protein, E coli; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C114650:OriC chromosomal replication origin; D051738:Origin Recognition Complex; D014764:Viral Proteins