Disposable polydimethylsiloxane/silicon hybrid chips for protein detection.
This paper presents disposable protein analysis chips with single- or four-chamber-constructed from poly(dimethylsiloxane) (PDMS) and silicon. The chips are composed of a multilayer stack of PDMS layers that sandwich a silicon microchip. This inner silicon chip features an etched array of micro-cavities hosting polymeric beads. The sample is introduced into the fluid network through the top PDMS layer, where it is directed to the bead chamber. After reaction of the analyte with the probe beads, the signal generated on the beads is captured with a CCD camera, digitally processed, and analyzed. An established bead-based fluorescent assay for C-reactive protein (CRP) was used here to characterize these hybrid chips. The detection limit of the single-chamber protein chip was found to be 1 ng/ml. Additionally, using a back pressure compensation method, the signals from each chamber of the four-chamber chip were found to fall within 10% of each other.
Li
Shifeng
S
Department of Mechanical Engineering, The University of Texas at Austin, 1 University Station, C2200 Austin, TX 78712, USA.
Floriano
Pierre N
PN
Christodoulides
Nicolaos
N
Fozdar
David Y
DY
Shao
Dongbing
D
Ali
Mehnaaz F
MF
Dharshan
Priya
P
Mohanty
Sanghamitra
S
Neikirk
Dean
D
McDevitt
John T
JT
Chen
Shaochen
S
D002097:C-Reactive Protein / Q000032:analysis* / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004209:Disposable Equipment; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry*; D012828:Silicones / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018511:Systems Integration
D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon; D002097:C-Reactive Protein; D012825:Silicon