[cDNA microarray and cluster analysis to identify the significance of immune genes associated with benzene poisoning].
OBJECTIVE
To delineate the immune regulatory pathway of benzene poisoning by using gene expression profile analysis.


METHODS
Peripheral white blood cell gene expression profile of 7 benzene poisoning patients, including one aplastic anemia, was determined by microarray. Seven chips from normal workers were served as controls. Cluster analysis of gene expression profile was performed. Differentially expressed immune genes associated with benzene poisoning were determined.


RESULTS
Among the 2 779 target genes, 38 genes differentially expressed were identified, including 10 up-regulated genes such as CD59, TRA@, MCP etc, and 14 down-regulated genes such as HLA-DMB, HLA-DQA1, HLA-DPB1, ITGB2, PFC etc. Cluster analysis showed that the expression profiles of 38 genes were associated with benzene poisoning.


CONCLUSIONS
Differentially expressed immune genes may play an important role in the pathogenesis of benzene poisoning.
Wang
Hong
H
Department of Occupational and Enironmental Health, School of Public Health, Wuhan University, Wuhan, 430071, China.
Bi
Yongyi
Y
Wang
Chunhong
C
Tao
Ning
N
Xia
Ying
Y
Zhang
Ling
L
D001554:Benzene / Q000506:poisoning*; D018957:CD59 Antigens / Q000235:genetics; D016022:Case-Control Studies; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D006681:HLA-D Antigens / Q000235:genetics; D059868:HLA-DP beta-Chains / Q000235:genetics; D059848:HLA-DQ alpha-Chains / Q000235:genetics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis
D018957:CD59 Antigens; D006681:HLA-D Antigens; C084245:HLA-DM antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D001554:Benzene