title: Determination of jasmonic acid in Lemna minor (L.) by liquid chromatography with fluorescence detection.
abstract: A new method is described for the determination of endogenous jasmonic acid (JA) in Lemna minor plant extracts using liquid chromatography (LC) with fluorescence detection. Plant tissues were extracted and derivatised using 9-anthryldiazomethane (ADAM reagent) prepared in situ. Accuracy and precision were improved by using the internal standard dihydrojasmonic acid (dh-JA) for the correction of JA losses during sample preparation steps. Liquid chromatography-mass spectrometry (LC/MS) analysis of ADAM derivatives of JA and dh-JA confirmed that a single molecule of JA and dh-JA was coupled with one molecule of reagent. Derivatives of JA and dh-JA were separated with gradient elution on a C18 reversed-phase column using acetonitrile/water as a mobile phase and detected by a fluorescence detector at excitation and emission wavelengths of 254 and 412 nm, respectively. The detection limits of JA and dh-JA were 2.9 ng mL(-1) and 3.7 ng mL(-1) per 50-microL injection. The method is reproducible and selective and yields single peaks for each compound regardless of isomer. The specificity and accuracy of the proposed LC/FD method was confirmed by liquid chromatography-TurboIon Spray tandem mass spectrometric (LC/MS/MS) analysis of free JA in Lemna minor samples under multiple reaction monitoring conditions.
authors.affiliation: Faculty of Agriculture, Vrbanska 30, 2000 Maribor, Slovenia. Janja.Kristl@uni-mb.si
authors.forename: Janja
authors.initials: J
authors.lastname: Kristl
authors.forename: Marjan
authors.initials: M
authors.lastname: Veber
authors.forename: Bozidar
authors.initials: B
authors.lastname: Krajnicic
authors.forename: Klara
authors.initials: K
authors.lastname: Oresnik
authors.forename: Metka
authors.initials: M
authors.lastname: Slekovec
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D003517:Cyclopentanes / Q000032:analysis*; D019684:Magnoliopsida / Q000737:chemistry*; D054883:Oxylipins; D010936:Plant Extracts / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010936:Plant Extracts; C011006:jasmonic acid