Culture-independent analysis of fecal microbiota in cattle.
The phylogenetic diversity of the fecal bacterial community in Holstein cattle was determined by 16S ribosomal RNA gene sequence analysis. The sequences were affiliated with the following phyla: Firmicutes (81.3%), Bacteroidetes (14.4%), Actinobacteria (2.5%), and Proteobacteria (1.4%). The Clostridium leptum subgroup was the most phylogenetically diverse group in cattle feces. In addition, a number of previously uncharacterized and unidentified bacteria were recognized in clone libraries.
Ozutsumi
Yuhei
Y
United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Hayashi
Hidenori
H
Sakamoto
Mitsuo
M
Itabashi
Hisao
H
Benno
Yoshimi
Y
D000818:Animals; D001419:Bacteria / Q000145:classification*; D002417:Cattle; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA; D010802:Phylogeny; D017421:Sequence Analysis / Q000379:methods*