Determination of cryptotanshinone and its metabolite in rat plasma by liquid chromatography-tandem mass spectrometry.
A sensitive and selective LC-MS-MS method has been developed and validated for the determination of cryptotanshinone (CTS) and its active metabolite tanshinone II A (TS II A) in rat plasma using fenofibrate (FOFB) as internal standard. Liquid-liquid extraction was used for sample preparation. Chromatographic separation was achieved on a Waters symmetry ODS column using methanol and water (85:15) as mobile phase delivered at 1.0 mL/min. LC-MS-MS analysis was carried out on a Finnigan LC-TSQ Quantum mass spectrometer using atmospheric pressure chemical ionization (APCI) and positive multiple reaction monitoring. Ions monitored were m/z 297.0--> 251.0 for CTS, m/z 295.0--> 249.0 for TS II A, and m/z 361.1--> 233.0 for FOFB with argon at a pressure of 0.2 Pa and collision energy of 25 eV for collision-induced dissociation (CID). The assay was linear over the range 0.1-20 ng/mL for CTS and 0.2-15 ng/mL for TS II A. The average recoveries of CTS and TS II A from rat plasma were 93.7 and 94.7%, respectively. The established method has been applied in a pharmacokinetic study of CTS in rats.
Song
M
M
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.
Hang
T J
TJ
Zhang
Zh X
ZhX
Du
R
R
Chen
J
J
D045784:Abietanes; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010616:Phenanthrenes / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D045784:Abietanes; D010616:Phenanthrenes; C021751:tanshinone; C037886:cryptotanshinone