title: Identification and analysis of U5 snRNA variants in Drosophila.
abstract: Distinct isoforms of spliceosomal RNAs may be involved in regulating pre-messenger RNA splicing in eukaryotic cells. During a large-scale effort to identify small noncoding RNAs in Drosophila, we isolated a U5 snRNA-like molecule containing a 5' segment identical to that of the canonical (major) U5 snRNA but with a variant Sm binding site and a distinct 3' hairpin sequence. Based on this finding, another six similar U5 snRNA-like sequences were identified within the Drosophila genome by sequence similarity to the invariant loop in the 5' half of U5. Interestingly, although all of these variants are expressed in vivo, each shows a distinct temporal expression profile during Drosophila development, and one is expressed primarily in fly heads. The presence of these U5 snRNA variants within RNP particles suggests their role in splicing and implies a possible connection to regulation of developmental and tissue-specific gene expression.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Dennis J
authors.initials: DJ
authors.lastname: Lullo
authors.forename: Enbo
authors.initials: E
authors.lastname: Ma
authors.forename: Susan E
authors.initials: SE
authors.lastname: Celniker
authors.forename: Donald C
authors.initials: DC
authors.lastname: Rio
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Doudna
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020033:Protein Isoforms / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017433:Protein Structure, Secondary; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D017411:Ribonucleoproteins, Small Nuclear / Q000378:metabolism; D017381:Spliceosomes; D055517:snRNP Core Proteins
chemical_list: D001324:Autoantigens; D020033:Protein Isoforms; D012342:RNA, Small Nuclear; D017411:Ribonucleoproteins, Small Nuclear; C405485:U5 small nuclear RNA; D055517:snRNP Core Proteins