Transcriptional characterizations of differences between eutopic and ectopic endometrium.
Endometriosis, defined as the presence of endometrial glandular and stromal cells outside the uterine cavity, is a common gynecological disease with poorly understood pathogenesis. Using laser capture microdissection and a cDNA microarray with 9600 genes/expressed sequence tags (ESTs), we have conducted a comprehensive profiling of gene expression differences between the ectopic and eutopic endometrium taken from 12 women with endometriosis adjusted for menstrual phase and the location of the lesions. With dye-swapping and replicated arrays, we found 904 genes/ESTs that are differentially expressed. We validated the gene expression using real-time RT-PCR. We found that the expression patterns of these genes/ESTs correctly classified the 12 patients into ovarian and nonovarian endometriosis. We identified gene clusters that are location-specific. In addition, we identified several biological themes using Expression Analysis Systematic Explorer. Finally, we identified 79 pathways with over 100 genes with known functions, which include oxidative stress, focal adhesion, Wnt signaling, and MAPK signaling. The identification of these genes and their associated pathways provides new insight. Our findings will stimulate future investigations on molecular genetic mechanisms underlying the pathogenesis of endometriosis.
Wu
Yan
Y
Department of Pediatrics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226-0509, USA.
Kajdacsy-Balla
André
A
Strawn
Estil
E
Basir
Zainab
Z
Halverson
Gloria
G
Jailwala
Parthav
P
Wang
Yuedong
Y
Wang
Xujing
X
Ghosh
Soumitra
S
Guo
Sun-Wei
SW
D001483:Base Sequence; D017931:DNA Primers; D004717:Endometrium / Q000378:metabolism*; D020224:Expressed Sequence Tags; D005260:Female; D006801:Humans; D008597:Menstrual Cycle; D020411:Oligonucleotide Array Sequence Analysis; D010049:Ovarian Diseases / Q000473:pathology; D011247:Pregnancy; D011271:Pregnancy, Ectopic / Q000235:genetics*; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D017931:DNA Primers