title: Immunohistochemical detection of antigens of distemper, adenovirus and parainfluenza viruses in domestic dogs with pneumonia.
abstract: The lungs of 35 dogs that died in Mexico from acute or subacute pneumonia were examined immunohistochemically for canine distemper virus (CDV), canine adenovirus (CAV) and canine parainfluenza virus (CpiV), to determine their frequency and occurrence and possible associations. CDV was identified in 27 (77%) cases, CAV in 20 (57%) and CpiV in 18 (51%). The most frequent dual association was that between CDV and CpiV (five cases; 14%). All three viruses, however, were identified in the same lung in 10 cases. Immunolabelling occurred in alveolar macrophages, monocytes, pneumocytes, epithelial cells and syncytial cells. It was concluded that immunohistochemistry is a useful diagnostic tool in canine respiratory disease to complement histopathological examination.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine and Husbandry, National Autonomous University of Mexico, 04510 Mexico City, Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Damián
authors.forename: E
authors.initials: E
authors.lastname: Morales
authors.forename: G
authors.initials: G
authors.lastname: Salas
authors.forename: F J
authors.initials: FJ
authors.lastname: Trigo
mesh_terms: D000257:Adenoviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary; D006516:Adenoviruses, Canine / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D004216:Distemper / Q000175:diagnosis / Q000276:immunology / Q000473:pathology; D004217:Distemper Virus, Canine / Q000276:immunology / Q000302:isolation & purification*; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology / Q000821:virology; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary*; D008168:Lung / Q000473:pathology / Q000821:virology; D008297:Male; D010252:Paramyxoviridae / Q000276:immunology / Q000302:isolation & purification*; D018184:Paramyxoviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary; D011024:Pneumonia, Viral / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D012189:Retrospective Studies
chemical_list: D000956:Antigens, Viral