title: An automated, pressure-driven sampling device for harvesting from liquid cultures for genomic and biochemical analyses.
abstract: Here we describe an automated, pressure-driven, sampling device for harvesting 10 to 30 ml samples, in replicate, with intervals as short as 10 s. Correlation between biological replicate time courses measured by microarrays was extremely high. The sampler enables sampling at intervals within the range of many important biological processes.
authors.affiliation: Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA.
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Aragon
authors.forename: Gabriel A
authors.initials: GA
authors.lastname: Quiñones
authors.forename: Chris
authors.initials: C
authors.lastname: Allen
authors.forename: Jason
authors.initials: J
authors.lastname: Thomas
authors.forename: Sushmita
authors.initials: S
authors.lastname: Roy
authors.forename: George S
authors.initials: GS
authors.lastname: Davidson
authors.forename: Peter D
authors.initials: PD
authors.lastname: Wentzell
authors.forename: Brian
authors.initials: B
authors.lastname: Millier
authors.forename: Jason E
authors.initials: JE
authors.lastname: Jaetao
authors.forename: Angelina L
authors.initials: AL
authors.lastname: Rodriguez
authors.forename: Margaret
authors.initials: M
authors.lastname: Werner-Washburne
mesh_terms: D001331:Automation; D003470:Culture Media; D004867:Equipment Design; D008828:Microbiological Techniques / Q000295:instrumentation*; D020411:Oligonucleotide Array Sequence Analysis; D012331:RNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D015203:Reproducibility of Results; D015003:Yeasts / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D003470:Culture Media; D012331:RNA, Fungal