title: In ovo inhibition of fowlpoxvirus replication by a gall extract from Guiera senegalensis.
abstract: Several field isolates of fowlpoxvirus (FPV) from Burkina Faso, West Africa, were isolated and partly evaluated by molecular analysis. In addition, the in ovo antiviral activity against FPV of a gall extract from Guiera senegalensis was determined. Three viral isolates were obtained from suspected fowlpox cases after passage in embryonating chicken eggs and their poxviral identity confirmed by electron microscopy. All isolates were found to be pathogenic for chicks and all grew well in cell culture. Polymerase chain reaction and sequencing of amplicons revealed sequences identical with those of other FPV strains. The most studied isolate was then employed for use in an antiviral assay. An aqueous acetone extract from the galls of G. senegalensis was found to inhibit both virus-induced pock formation and to reduce viral titre in embryonating chicken eggs. The suggested mechanism of action is the activation of the alternative complement pathway and the inhibition of FPV-induced cholesterogenesis in ovo by constituents of the gall extract.
authors.affiliation: Laboratoire de Biochimie et Chimie appliquées, UFR/SVT, Université de Ouagadougou, 03 BP 7021, Ouagadougou 03, Burkina Faso. celamien@hotmail.com
authors.forename: C E
authors.initials: CE
authors.lastname: Lamien
authors.forename: J
authors.initials: J
authors.lastname: Mans
authors.forename: A
authors.initials: A
authors.lastname: Meda
authors.forename: E
authors.initials: E
authors.lastname: Couacy-Hymann
authors.forename: M
authors.initials: M
authors.lastname: Romito
authors.forename: A G
authors.initials: AG
authors.lastname: Ouedraogo
authors.forename: O G
authors.initials: OG
authors.lastname: Nacoulma
authors.forename: G J
authors.initials: GJ
authors.lastname: Viljoen
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use; D002050:Burkina Faso; D002642:Chick Embryo; D029764:Combretaceae / Q000737:chemistry*; D005586:Fowlpox / Q000188:drug therapy; D005587:Fowlpox virus / Q000187:drug effects*; D010063:Ovum / Q000821:virology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010941:Plant Tumors; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts