[The interaction of vitamin B6 with the human serum albumin].
The Human Serum Albumin (HSA) can emit fluorescence (lamda(em) = 350 nm) under irradiation of ultraviolet light (lamda(ex) = 296 nm). After the vitamin B6 (B6) was added into HSA solution the fluorescence of HSA was quenched partially. This quenching was static quenching owing to the fact that ro (fluorescence life time (life time of the molecule excitation state) of HSA without B6) equaled to tau(i) (fluorescence life time of HSA with B6). The formation constant K between HSA and B6 was observed from the experiment, and then the binding distance R0 was determined (R0 = 1.872 nm). The CD spectra of samples (HSA, [HSA] = 1 x 10(-5) mol x L(-1), HSA +B6, [B6] = 5 x 10(-8), 1.5 x 10(-7), 2.5 x 10(-7), 3.5x 10(-7), 4.5x 10(-7) mol x L(-1)) were measured, and all CD spectra were almost the same. From [theta] values, the contents (percent content) of four structure(alpha-Helix, beta-Pleated, Beta-corner, and Randon wind) of samples can be calculated, and we find that all samples contain almost the same structure contents.
Wang
Jian-lin
JL
School of physics, Peking University, Beijing 100871, China
Fu
Lian-chun
LC
Zhou
Shi-wu
SW
Chen
Zhi-jian
ZJ
Lu
Wen-bo
WB
Ye
Xue-min
XM
Meng
Guang-zheng
GZ
Song
Zeng-fu
ZF
D000465:Algorithms; D001665:Binding Sites; D002942:Circular Dichroism; D005453:Fluorescence; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D025101:Vitamin B 6 / Q000737:chemistry* / Q000378:metabolism
D012709:Serum Albumin; D025101:Vitamin B 6