[Inoculation of plasmid expressing the dengue 2 NS1 gene elicits immunity in mice].
OBJECTIVE
To study cellular and humoral immune responses to NS1 protein in mice inoculated intramuscularly with recombinant plasmid expressing dengue 2 NS1 gene.


METHODS
The eukaryotic expressing plasmid pCNX2-NS1 was injected into tibialis anterior muscle in mice. The mice were subsequently boosted with the same dose and same method twice after the initial inoculation. The mice were killed at four-week intervals and their serum and spleen cells were harvested for further test.


RESULTS
Dengue 2 antibodies were detectable in the sera from inoculated animals four weeks after the last boost. The changes of CD4+ T lymphocyte and CD8+ T lymphocyte were also determined by flow cytometry.


CONCLUSIONS
The recombinant plasmid containing dengue 2 NS1 genes is immunogenic in intramuscularly inoculated mice. The vaccinated mice produced dengue-2 specific and long lasting immunity.
Yang
Chun-yu
CY
Department of Microbiology, Sun Yat-sen University of Medical Sciences, Guangzhou, China. ychunyucn@yahoo.com.cn
Chen
Yuan
Y
Yan
Hui-jun
HJ
Fang
Dan-yun
DY
Xue
Yao-hua
YH
Jiang
Li-fang
LF
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D003715:Dengue / Q000097:blood / Q000276:immunology* / Q000821:virology; D053059:Dengue Vaccines / Q000276:immunology; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D017778:Vaccines, Combined / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000235:genetics*
D000914:Antibodies, Viral; D053059:Dengue Vaccines; D017778:Vaccines, Combined; D019444:Vaccines, DNA; D017361:Viral Nonstructural Proteins