Use of a selective enrichment broth to recover Clostridium difficile from stool swabs stored under different conditions.
The recovery of Clostridium difficile from the stools of patients with C. difficile-associated diarrhea was evaluated by use of an enrichment broth (cycloserine-cefoxitin fructose broth supplemented with 0.1% sodium taurocholate [TCCFB]) and was compared to that from selective agar (cycloserine-cefoxitin fructose agar [CCFA]) and alcohol shock followed by inoculation onto blood agar (AS-BA). TCCFB was superior to CCFA and AS-BA, and neither the storage time nor the storage temperature affected the recovery rate.
Arroyo
Luis G
LG
Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada. larroyo@uoguelph.ca
Rousseau
Joyce
J
Willey
Barbara M
BM
Low
Don E
DE
Staempfli
Henry
H
McGeer
Allison
A
Weese
J Scott
JS
D001431:Bacteriological Techniques; D016360:Clostridioides difficile / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D003967:Diarrhea / Q000382:microbiology*; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D013048:Specimen Handling / Q000379:methods*
D003470:Culture Media