Cytotoxic effects of Cochlospermum regium (Mart & Schrank) Pilger aqueous root extract on mammalian cells.
We investigated the effect of Cochlospermum regium (Mart & Schrank) Pilger aqueous root extract on Chinese hamster ovarian (CHO)-K1 cells. The extract significantly decreased proliferation of CHO-K1 cells (EC(50)=1.5mg/mL). Apoptosis induction was analysed by fluorescent microscopy. Cell cultures treated with Cochlospermum regium extract for 4h contained 13.6% apoptotic cells after 24h (investigated by fluorescent DNA-microscopy with acridine orange/ethidium bromide staining). Characteristic chromatin condensation and fragmentation, verified by 4',6-diamidino-2-phenylindole (DAPI) staining, was observed in the cells after treatment with Cochlospermum regium extract. The results confirm the toxicity of Cochlospermum regium root extract to immortal, non-tumorigenic mammalian cells in vitro.
Ceschini
Livônios
L
Departamento de Biologia Celular, Laboratório de Biologia Molecular, Universidade de Brasília, Brasília, Distrito Federal CEP 70910-900, Brazil.
Campos
Elida Geralda
EG
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D031212:Bixaceae; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D010936:Plant Extracts / Q000302:isolation & purification / Q000633:toxicity*; D018517:Plant Roots
D010936:Plant Extracts