The use of a supercooling refrigerator improves the preservation of organ grafts.
Current medical transplantation confronts major problems such as the shortage of donors and geographical restrictions that inhibit efficient utilization of finite donor organs within their storage lives. To overcome these issues, expanding organ preservation time has become a major concern. We investigated whether a strategy which best preserves organ grafts can be achieved by the use of a newly developed refrigerating chamber, which is capable of establishing a supercooled and unfrozen state stably by generating an electrostatic field in its inside. When adult rat organs such as heart, liver, and kidneys were stored in the supercooled conditions, the levels of major biochemical markers leaked from the preserved organs were significantly lower than in the ordinary hypothermic storage. No apparent tissue damages were observed histologically after the supercooled preservation. Our results suggest that the use of this supercooling refrigerator improves organ preservation and may provide an innovative technique for human organ transplantation.
Monzen
Koshiro
K
Department of Cardiovascular Medicine, University of Tokyo Graduate School of Medicine, Bunkyo-ku, Japan.
Hosoda
Toru
T
Hayashi
Doubun
D
Imai
Yasushi
Y
Okawa
Yasuhiro
Y
Kohro
Takahide
T
Uozaki
Hiroshi
H
Nishiyama
Tomoki
T
Fukayama
Masashi
M
Nagai
Ryozo
R
D000818:Animals; D005615:Freezing; D006652:Histological Techniques; D006801:Humans; D016030:Kidney Transplantation; D016031:Liver Transplantation; D016040:Lung Transplantation; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D012034:Refrigeration; D013997:Time Factors; D009927:Tissue and Organ Procurement; D019737:Transplants