Chitosan microspheres as immobilized dye affinity support for catalase adsorption.
Chitosan microsphere (CS) was prepared by phase-inversion method as the support matrices. Cibacron Blue F3GA (CB) was covalently attached to the chitosan microspheres, and thus the novel dye-affinity adsorbent was obtained. These Cibacron Blue F3GA-attached chitosan microspheres (CB-CS) were used in the catalase (CAT) adsorption studies. The maximum CAT adsorption capacity of Cibacron Blue F3GA-attached chitosan microspheres was 28.4 mg/g at pH 7.0. Langmuir adsorption model was found to be applicable in interpreting CAT adsorption. Significant amount of the adsorbed CAT (up to 90.6%) was eluted in the elution medium containing 0.5 M NaSCN at pH 8.0. It appears that CB-CS can be applied for adsorption of CAT without causing any denaturation.
Shentu
Jingling
J
Department of Chemistry, Zhejiang University, ZheDa Road 38, Hangzhou, Zhejiang 310027, China.
Wu
Jianmin
J
Song
Weihua
W
Jia
Zhishen
Z
D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D002374:Catalase / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D002845:Chromatography; D002846:Chromatography, Affinity; D004305:Dose-Response Relationship, Drug; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006418:Heme / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D008024:Ligands; D046911:Macromolecular Substances / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D013997:Time Factors
D001672:Biocompatible Materials; D002614:Chelating Agents; D004800:Enzymes, Immobilized; D007477:Ions; D008024:Ligands; D046911:Macromolecular Substances; D006418:Heme; D048271:Chitosan; D002374:Catalase