Immunophenotypic profile of lymphoplasmacytic lymphoma/Waldenström macroglobulinemia.
We retrospectively reviewed the immunophenotypic profile of 75 cases of lymphoplasmacytic lymphoma/Waldenström macroglobulinemia (LPL/WM) analyzed by flow cytometry. All patients had monoclonal IgM (median, 2,100 mg/dL [21 g/L]) in serum and were considered clinically to have WM. The neoplastic cells, in all cases, expressed monoclonal immunoglobulin light chain (k, 55; l, 20) and CD19, and every case assessed was positive for CD20 (n=68) and CD52 (n=60). The results for other antigens assessed in decreasing frequency of positivity were as follows: surface IgM (26/28 [93%]), CD79b (11/13 [85%]), CD11c (13/16 [81%]), CD25 (5/7 [71%]), CD23 (17/28 [61%]), CD38 (24/50 [48%]), FMC7 (11/29 [38%]), CD22 (4/12 [33%]), CD5 (3/65 [5%]), and CD10 (1/38 [3%]). These results show that the immunophenotype of LPL/WM is variable and overlaps with other B-cell lymphoproliferative disorders. CD23, usually of dim intensity, and CD11c are expressed commonly in LPL/WM. Rare CD5+ and CD10+ cases of LPL/WM also exist.
Konoplev
Sergej
S
Department of Hematopathology, The University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
Medeiros
L Jeffrey
LJ
Bueso-Ramos
Carlos E
CE
Jorgensen
Jeffrey L
JL
Lin
Pei
P
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D018956:CD5 Antigens / Q000032:analysis; D000074301:CD52 Antigen; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015260:Neprilysin / Q000032:analysis; D017455:Receptors, IgE / Q000032:analysis; D012189:Retrospective Studies; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology* / Q000473:pathology
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D018956:CD5 Antigens; D000074301:CD52 Antigen; C000613862:CD52 protein, human; D006023:Glycoproteins; D017455:Receptors, IgE; D015260:Neprilysin