title: Radiation responses of perfused tracheal tissue.
abstract: We are using a novel perfusion system to examine the effects of radiation on a model respiratory tissue. Tracheas taken from young adult male Fischer 344 rats are embedded in a growth factor-enriched agarose matrix that is mounted in a special apparatus designed to allow growth medium to periodically wash the epithelial surface of the lumen. A comparison of the microarray expression profiles of freshly harvested tracheas and tracheas maintained in perfusion culture for 24 h shows no significant difference except for an increase in expression of a few metabolism- and surfactant-related genes. Perfusion culture samples exposed to 4 Gy of X rays show a lower than expected increase in expression for some cell cycle- and repair-related genes.
authors.affiliation: Department of Nuclear Engineering, Texas A&M University, College Station, Texas 77843-3133, USA. ford@ne.tamu.edu
authors.forename: J R
authors.initials: JR
authors.lastname: Ford
authors.forename: A J
authors.initials: AJ
authors.lastname: Maslowski
authors.forename: R A
authors.initials: RA
authors.lastname: Redd
authors.forename: L A
authors.initials: LA
authors.lastname: Braby
mesh_terms: D000818:Animals; D015870:Gene Expression / Q000528:radiation effects; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010477:Perfusion; D051381:Rats; D011916:Rats, Inbred F344; D014132:Trachea / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*