Effects of alterations of glomerular fibrin deposition on renal inflammation in rats at different age stages.
Recent data indicated that aging accelerated glomerular fibrin deposition induced by lipopolysaccharide (LPS) in mice. Our hypothesis was that aging may exacerbate glomerular inflammatory responses induced by glomerular fibrin deposition. Both young and aged rats with glomerular fibrin deposition induced by LPS were treated with tranexamic acid (TA) and TA plus urokinase (UK). Infiltrating inflammatory cells and expressions of monocyte chemoattractant protein 1, intercellular adhesion molecule 1, and vascular endothelial-cadherin were markedly upregulated in the LPS+TA group compared with the LPS group. Reduction of fibrin deposition in the LPS+TA+UK group was associated with downregulation of the above indices (p < .05), whereas the alteration of vascular endothelial-cadherin protein expression was negatively correlated with the fibrin deposition. There were also significant differences in increased expressions of monocyte chemoattractant protein 1 and intercellular adhesion molecule 1 between young and aged rats. These in vivo data demonstrated that fibrin deposition contributed to glomerular inflammatory responses, which could be exacerbated by aging.
Xi
Chunsheng
C
Department of Nephrology, Kidney Center and Key Lab of PLA, General Hospital of PLA, Fuxing Road 28, Beijing 100853, People's Republic of China.
Chen
Xiangmei
X
Sun
Xuefeng
X
Shi
Suozhu
S
Feng
Zhe
Z
Wang
Jianzhong
J
Hong
Quan
Q
Lu
Yang
Y
Lin
Shupeng
S
D000375:Aging; D000818:Animals; D000933:Antifibrinolytic Agents / Q000494:pharmacology; D015703:Antigens, CD; D015152:Blotting, Northern; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics; D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D005337:Fibrin / Q000378:metabolism*; D005921:Glomerulonephritis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D007678:Kidney Glomerulus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014148:Tranexamic Acid / Q000494:pharmacology; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology; D000099152:Cadherin 5
D000933:Antifibrinolytic Agents; D015703:Antigens, CD; D015820:Cadherins; D018932:Chemokine CCL2; D005337:Fibrin; D018799:Intercellular Adhesion Molecule-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014148:Tranexamic Acid; D014568:Urokinase-Type Plasminogen Activator; D000099152:Cadherin 5