Convergent synthesis of peptide nucleic acids by native chemical ligation.
[reaction: see text] A convergent strategy for synthesizing long contiguous PNA by a native chemical ligation-like technique of PNA segment couplings is presented. This approach required the synthesis of a new PNA-monomer featuring a 1-amino-2-thiol group. It is shown that the additional mercaptomethyl group leaves the hybridization properties of PNA ligation products unaffected. Furthermore, rapid and efficient fluorescence labeling of the ligation products is demonstrated.
Dose
Christian
C
Humboldt-Universität zu Berlin, Institut für Chemie, Brook-Taylor-Strasse 2, D-12489 Berlin, Germany.
Seitz
Oliver
O
D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D044366:Transition Temperature
D020135:Peptide Nucleic Acids