title: FRankenstein becomes a cyborg: the automatic recombination and realignment of fold recognition models in CASP6.
abstract: In the course of CASP6, we generated models for all targets using a new version of the "FRankenstein's monster approach." Previously (in CASP5) we were able to build many very accurate full-atom models by selection and recombination of well-folded fragments obtained from crude fold recognition (FR) results, followed by optimization of the sequence-structure fit and assessment of alternative alignments on the structural level. This procedure was however very arduous, as most of the steps required extensive visual and manual input from the human modeler. Now, we have automated the most tedious steps, such as superposition of alternative models, extraction of best-scoring fragments, and construction of a hybrid "monster" structure, as well as generation of alternative alignments in the regions that remain poorly scored in the refined hybrid model. We have also included the ROSETTA method to construct those parts of the target for which no reasonable structures were generated by FR methods (such as long insertions and terminal extensions). The analysis of successes and failures of the current version of the FRankenstein approach in modeling of CASP6 targets reveals that the considerably streamlined and automated method performs almost as well as the initial, mostly manual version, which suggests that it may be a useful tool for accurate protein structure prediction even in the hands of nonexperts.
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Kosinski
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Michal J
authors.initials: MJ
authors.lastname: Gajda
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Iwona A
authors.initials: IA
authors.lastname: Cymerman
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Michal A
authors.initials: MA
authors.lastname: Kurowski
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Pawlowski
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Boniecki
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Obarska
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Papaj
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Sroczynska-Obuchowicz
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Karolina L
authors.initials: KL
authors.lastname: Tkaczuk
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Sniezynska
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Sasin
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Augustyn
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Janusz M
authors.initials: JM
authors.lastname: Bujnicki
authors.affiliation: International Institute of Molecular and Cell Biology, Warsaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Feder
mesh_terms: D000465:Algorithms; D001331:Automation; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D003201:Computers; D030562:Databases, Protein; D008958:Models, Molecular; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D016415:Sequence Alignment; D012984:Software