title: Protein 4.1 G localizes in rodent microglia.
abstract: Although it was reported that protein 4.1 G, a cytoskeletal protein characterized by its general expression in the body, interacts with some signal transduction molecules in the central nervous system (CNS), its distribution and significance in vivo remained to be elucidated. In the present study, we have identified 4.1 G-positive cells in the rodent CNS, and demonstrated its immunolocalization in the developing mouse CNS. In the rodent CNS, 4.1 G was colocalized with markers for microglia, such as CD45, OX-42 and ionized calcium-binding adapter molecule 1 (Iba1), but not with markers for neuronal or other glial cells. Additionally, colocalization of 4.1 G and A1 adenosine receptor was observed in the mouse cerebrum. In a mixed glial culture, most OX-42-positive microglia were positive for 4.1 G, and 4.1 G isoforms of the same molecular weight as in the rat brain were expressed in cultured microglia, where 4.1 G mRNA was detected by RT-PCR. In the developing mouse cerebral cortex, 4.1 G was detected in immature microglia, which were positive for Iba1. These results indicate that 4.1 G in the CNS is mainly distributed in microglia in vivo. Considering the interactions between 4.1 G and the signal transduction molecules, putative roles have been proposed for 4.1 G in microglial functions in the CNS.
authors.affiliation: Department of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Tamaho, Yamanashi, 409-3898, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Ohno
authors.forename: Nobuo
authors.initials: N
authors.lastname: Terada
authors.forename: Junya
authors.initials: J
authors.lastname: Tanaka
authors.forename: Akiko
authors.initials: A
authors.lastname: Yokoyama
authors.forename: Hisashi
authors.initials: H
authors.lastname: Yamakawa
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Fujii
authors.forename: Takeshi
authors.initials: T
authors.lastname: Baba
authors.forename: Osamu
authors.initials: O
authors.lastname: Ohara
authors.forename: Shinichi
authors.initials: S
authors.lastname: Ohno
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology; D002540:Cerebral Cortex / Q000166:cytology; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000166:cytology* / Q000378:metabolism; D020033:Protein Isoforms / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D015398:Signal Transduction / Q000502:physiology
chemical_list: D003598:Cytoskeletal Proteins; C480491:Epb41l2 protein, rat; D020033:Protein Isoforms