Polyphenol interaction with the T47D human breast cancer cell line.
Experimental and epidemiological studies indicate that antioxidant food polyphenols could have antimitotic activities, interfering with cancer initiation, progression or mortality. Circulating polyphenols are far lower than the nominal value in foods. In the rare studies dealing with polyphenol bioavailability, it was noted that their active concentrations in the blood are <1% of their food concentration. In the present study we investigated the effect of four polyphenols (resveratrol, and the flavonoids quercetin, catechin and epicatechin, major constituents of wine) in the hormone-sensitive human cancer cell line T47D, at concentrations compatible with their calculated plasma concentrations after ingestion of a moderate quantity of wine (nM or pM). Our results indicate that cell growth was decreased, with cells being arrested at the S phase of the cycle. In addition, we provide evidence of a bimodal modulation of the NO/NOS system, affecting its activity and transcription. We show that modulation of this system is sufficient to explain polyphenol action on this cell line. This result suggests a potential importance of wine ingestion and possibly the consumption of other polyphenol-rich dietary foods and drinks in the control of breast cancer cell growth.
Nifli
Artemissia-Phoebe
AP
Laboratory of Experimental Endocrinology, University of Crete, School of Medicine, Heraklion, 71003, Greece.
Kampa
Marilena
M
Alexaki
Vassilia-Ismini
VI
Notas
George
G
Castanas
Elias
E
D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D002392:Catechin / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D011794:Quercetin / Q000494:pharmacology; D000077185:Resveratrol; D016196:S Phase / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology; D014920:Wine / Q000032:analysis
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013267:Stilbenes; D009569:Nitric Oxide; D002392:Catechin; D011794:Quercetin; D019001:Nitric Oxide Synthase; D000077185:Resveratrol