Effects of a free radical scavenger, MCI-186, on ischemia-reperfusion injury during extended liver resection in dogs.
OBJECTIVE
In liver surgery, total clamping of the portal triad (Pringle's procedure) is commonly used, and this sometimes causes liver failure. This study evaluated the effects of a free radical scavenger, MCI-186, on ischemia-reperfusion injury during liver resection in dogs.


METHODS
The experimental animals were divided into two groups. In the MCI group (n = 6), MCI-186 (6mg/kg/h) was administered twice, through a catheter placed in the right hepatic vein: the first time was from 0.5 hours before the onset of ischemia until ischemia by partial inflow occlusion, and the second was from 0.5-hours before reperfusion until reperfusion. In the control group (n = 6), vehicle (physiological saline) was administered in the same manner.


RESULTS
The serum AST, ALT, and LDH levels were significantly (P < 0.05) lower in the MCI group than in the control group. Hepatic tissue blood flow 0.5 hours after reperfusion was significantly (P < 0.05) higher in the MCI group than in the control group. Histological tissue damage was mild, and tissue MDA levels were significantly (P < 0.05) lower in the MCI group than in the control group.


CONCLUSIONS
MCI-186 ameliorates the ischemia-reperfusion injury caused by Pringle's procedure during extended liver resection.
Totsuka
Osamu
O
Second Department of Surgery, Gunma University School of Medicine, Gunma, Japan.
Takeyoshi
Izumi
I
Tsutsumi
Hirofumi
H
Arakawa
Kazuhisa
K
Akao
Takahiko
T
Muraoka
Masato
M
Ohwada
Susumu
S
Matsumoto
Koshi
K
Morishita
Yasuo
Y
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D004285:Dogs; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D006498:Hepatectomy; D015227:Lipid Peroxidation; D008099:Liver / Q000098:blood supply; D008833:Microcirculation; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000503:physiopathology
D016166:Free Radical Scavengers; D000077553:Edaravone; D000983:Antipyrine