Efficient production, purification, and application of egg yolk antibodies against human HLA-A*0201 heavy chain and light chain (beta2m).
The importance of eggs as a source of specific antibodies is well recognized. Egg yolk contains 8--20mg immunoglobulins (IgY) per milliliter. However, the major problem in separating IgY is to remove the high concentrations of lipids in egg yolk. We first used water dilution method to get the supernatant containing IgY, then purified the antibody by caprylic acid-ammonium sulfate method, and obtained specific antibody with satisfactory purity and activity. By comparison of these several methods, each has its advantages, one can be chosen to purify IgY according to practical need. The purified IgY produced by the immunized chickens can stain the human peripheral blood mononuclear cell effectively when labeled with fluorescent FITC.
Ruan
Guang-Ping
GP
Institute of Molecular Immunology, Southern Medical University, Guangzhou 510515, PR China. ruangp2002@hotmail.com
Ma
Li
L
He
Xiao-Wei
XW
Meng
Min-Jie
MJ
Zhu
Yong
Y
Zhou
Ming-Qian
MQ
Hu
Zhi-Ming
ZM
Wang
Xiao-Ning
XN
D000818:Animals; D002645:Chickens / Q000276:immunology; D004530:Egg Yolk / Q000737:chemistry* / Q000276:immunology; D015234:HLA-A Antigens / Q000276:immunology; D015789:HLA-A2 Antigen; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D001613:beta 2-Microglobulin / Q000276:immunology
D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; C016512:IgY; D007136:Immunoglobulins; D001613:beta 2-Microglobulin