title: [Construction of infectious Japanese encephalitis virus clone based on the cDNA template of the attenuated live vaccine production strain SA14-14-2].
abstract: OBJECTIVE
  To construct infectious Japanese encephalitis virus (JEV) based on the in vitro-ligated cDNA template of the vaccine strain SA14-14-2, and identify the virus.
  
  
  METHODS
  Full-length genomic cDNA of JEV SA14-14-2 strain was ligated and then RNA was transcribed in vitro, the infective virus was obtained by transfecting the RNA into Vero cells and identified.
  
  
  RESULTS
  The infective clone of JEV was constructed, the virulence was weaker than the wild virus.
  
  
  CONCLUSIONS
  It was possible to construct infectious clone from the production strain of live attenuated Japanese B encephalitis vaccine.
authors.affiliation: National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zeng
authors.forename: Li-li
authors.initials: LL
authors.lastname: Jia
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Yu
authors.forename: Guan-mu
authors.initials: GM
authors.lastname: Dong
authors.forename: Wen-xue
authors.initials: WX
authors.lastname: Liu
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Wang
authors.forename: De-fu
authors.initials: DF
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D004672:Encephalitis, Japanese / Q000473:pathology / Q000821:virology; D016679:Genome, Viral; D022321:Japanese Encephalitis Vaccines / Q000276:immunology; D051379:Mice; D012367:RNA, Viral / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000276:immunology; D014709:Vero Cells; D014774:Virulence
chemical_list: D018076:DNA, Complementary; D022321:Japanese Encephalitis Vaccines; D012367:RNA, Viral; D014613:Vaccines, Attenuated