title: Follicle-stimulating hormone receptor gene mutations are not evident in Greek women with premature ovarian failure and poor responders.
abstract: OBJECTIVE
  This clinical and molecular study aimed to investigate the presence of follicle-stimulating hormone (FSH) receptor gene mutations in women with premature ovarian failure (POF) and poor responders to in vitro fertilization treatment.
  
  
  METHODS
  DNA was extracted from blood samples for subsequent polymerase chain reaction (PCR). PCR was followed by restriction fragment length polymorphism and direct sequencing.
  
  
  RESULTS
  No inactivating mutations reported so far were identified in exons 6, 7, and 10 in women with POF and poor responders.
  
  
  CONCLUSIONS
  FSH receptor gene mutations are not frequent in Greek patients with POF as is the case in the rest of the world except for cases with ovarian dysgenesis in Finland.
authors.affiliation: Division of Human Reproduction, IVF Unit, 1st Department of Obstetrics and Gynecology, Athens University Medical School, Greece.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Loutradis
authors.forename: Eleni
authors.initials: E
authors.lastname: Patsoula
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Stefanidis
authors.forename: Peter
authors.initials: P
authors.lastname: Drakakis
authors.forename: Georgios
authors.initials: G
authors.lastname: Antonakis
authors.forename: Ritsa
authors.initials: R
authors.lastname: Bletsa
authors.forename: Aris
authors.initials: A
authors.lastname: Antsaklis
authors.forename: Stylianos
authors.initials: S
authors.lastname: Michalas
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D004247:DNA / Q000097:blood / Q000737:chemistry; D005091:Exons; D005260:Female; D006115:Greece / Q000453:epidemiology; D006801:Humans; D009154:Mutation; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016649:Primary Ovarian Insufficiency / Q000453:epidemiology / Q000235:genetics*; D011962:Receptors, FSH / Q000235:genetics*
chemical_list: D011962:Receptors, FSH; D004247:DNA