Sau-PCR, a novel amplification technique for genetic fingerprinting of microorganisms.
The proposed technique is based on the digestion of genomic DNA with the restriction endonuclease Sau3AI and subsequent amplification with primers whose core sequence is based on the Sau3AI recognition site. The method was tested on strains of lactic acid bacteria but could be proposed for virtually any culturable organism from which DNA can be extracted.
Corich
Viviana
V
Dipartimento di Biotecnologie Agrarie, Università di Padova, viale dell'Università 16, I-35020 Legnaro, Padova, Italy.
Mattiazzi
Alessandro
A
Soldati
Elisa
E
Carraro
Angela
A
Giacomini
Alessio
A
D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006095:Gram-Positive Cocci / Q000145:classification* / Q000235:genetics; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D015014:Yogurt / Q000382:microbiology
D017931:DNA Primers; D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases