Analysis of a single nucleotide polymorphism in codon 388 of the FGFR4 gene in malignant gliomas.
The FGFR4 codon 388 polymorphism (Arg(388), Arg/Gly(388) or Gly(388)) was determined in glioblastoma multiforme (GBM), anaplastic astrocytomas (AA), diffuse astrocytomas (DA), and control muscles. Arg(388) was rare in AA, GBM, muscles, and was absent in DA. The Arg/Gly(388) and the Gly(388) frequency was equal among GBM and controls. FGFR4 expression was not related to codon 388 in GBM, and no survival differences between Arg/Gly(388) and Gly(388) tumors were found. U87 cells (Arg/Gly(388)) did not show higher invasion than U138 cells (Gly(388)). This suggests that the FGFR4 codon 388 status does not play a major role in malignant gliomas.
Mawrin
Christian
C
Department of Neuropathology, Otto-von-Guericke-University, Leipziger Strasse 44, D-39120 Magdeburg, Germany. christian.mawrin@medizin.uni-magdeburg.de
Kirches
Elmar
E
Diete
Sabine
S
Wiedemann
Falk R
FR
Schneider
Thomas
T
Firsching
Raimund
R
Kropf
Siegfried
S
Bogerts
Bernhard
B
Vorwerk
Christian K
CK
Krüger
Sabine
S
Dietzmann
Knut
K
D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D003062:Codon; D017931:DNA Primers; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D051499:Receptor, Fibroblast Growth Factor, Type 4 / Q000235:genetics*
D003062:Codon; D017931:DNA Primers; C496371:FGFR4 protein, human; D051499:Receptor, Fibroblast Growth Factor, Type 4