Inactivation of Cryptosporidium parvum by ultrasonic irradiation.
The inactivation of Cryptosporidium parvum was investigated by the use of three different sonicators utilizing the squeeze-film effect, which may occur when ultrasound is irradiated into an extremely thin space and generate intensified pressure in the sample suspension. To expand from the small-scale horn-type sonicator to large-scale cylindrical or cleaning bath sonicators, the inactivation effectwas improved. In the case of the cylindrical sonicator (26.6 kHz, 30 W), 97% of the initial concentration of 2260 oocysts mL(-1) was inactivated at33 mL min(-1) (residence time of approximately 5.2 min). Hundreds of cubic meters of water can be treated per day at several kW using this sonicator. In addition, the simultaneous use of sonication and chlorination showed a beneficial effect on inactivation for C. parvum based on the evaluation of infectivity testing and morphological observation.
Oyane
I
I
Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan. tikuko@mtr.osakafu-u.ac.jp
Furuta
M
M
Stavarache
C E
CE
Hashiba
K
K
Mukai
S
S
Nakanishi
J M
JM
Kimata
I
I
Maeda
Y
Y
D000818:Animals; D002138:Calibration; D017606:Chlorine Compounds / Q000494:pharmacology*; D016785:Cryptosporidium parvum / Q000378:metabolism*; D004203:Disinfection / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D051379:Mice; D016513:Mice, SCID; D033661:Oocysts; D010087:Oxides / Q000494:pharmacology*; D011312:Pressure; D013010:Sonication; D013997:Time Factors; D014465:Ultrasonics; D014867:Water / Q000032:analysis
D017606:Chlorine Compounds; D007211:Indoles; D010087:Oxides; D014867:Water; C007293:DAPI; D016650:Fluorescein-5-isothiocyanate