[Mechanisms of capsaicin-induced apoptosis of human melanoma A375-S2 cells].
OBJECTIVE
To study the mechanisms of capsaicin-induced apoptosis of human melanoma A375-S2 cells.


METHODS
MTT assay, fluorescence microscopy, DNA agarose gel electrophoresis, flow cytometry and Western blot analysis were carried out to assess the morphological and biochemical changes of A375-S2 cells after capsaicin treatment.


RESULTS
Capsaicin induced A375-S2 cell death in a time- and dose-dependent manner. Sub-diploid peak was seen at 24 h after 250 micromol/L capsaicin treatment, and apoptotic bodies and DNA ladder were observed at 36 h after capsaicin treatment. The expression of inhibitor of caspase activated DNase (ICAD) was reduced with the lapse of time.


CONCLUSIONS
Capsaicin induces A375-S2 cell apoptosis and down-regulation of ICAD contributes to this process.
Gong
Xian-feng
XF
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
Wang
Min-wei
MW
Ikejima
Takashi
T
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis; D002211:Capsaicin / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008545:Melanoma / Q000473:pathology*; D015398:Signal Transduction; D012878:Skin Neoplasms / Q000473:pathology*
D000972:Antineoplastic Agents, Phytogenic; D051017:Apoptosis Regulatory Proteins; C109664:caspase-activated DNase inhibitor; D020169:Caspases; D002211:Capsaicin