title: Genetic diversity on 16S rDNA sequence and phylogenic tree analysis in Pasteurella pneumotropica strains isolated from laboratory animals.
abstract: To reveal the genetic diversity of Pasteurella pneumotropica, the 16S rDNA sequence and multiple alignments were performed for 35 strains (from 17 mice, 13 rats, 3 hamsters, 1 rabbit, and 1 guinea pig) identified as P. pneumotropica using a commercial biochemical test kit or PCR test and two reference strains (ATCC 35149 and CNP160). Each strain showed a close similarity with one of the following organisms: P. pneumotropica (M75083), Bisgaard taxon22 (AY172726), Pasteurella sp. MCCM00235 (AF224300), Pasteurellaceae gen. sp. Forsyth A3 (AF224301), and Actinobacillus muris (AF024526) on GenBank, and were divided into six clusters on a phylogenic tree. Two reference strains, P. pneumotropica biotype Jawetz and Heyl, were classified at both ends of the clusters. Our conclusion is that P. pneumotropica should be reclassified because of the very wide genetic diversity that exists.
authors.affiliation: ICLAS Monitoring Center, Central Institute for Experimental Animals, Miyamae-ku, Kawasaki-shi, Japan. nubuh@ciea.or.jp
authors.forename: Nobuhito
authors.initials: N
authors.lastname: Hayashimoto
authors.forename: Akira
authors.initials: A
authors.lastname: Takakura
authors.forename: Toshio
authors.initials: T
authors.lastname: Itoh
mesh_terms: D000818:Animals; D000830:Animals, Laboratory / Q000382:microbiology*; D006224:Cricetinae; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis*; D014644:Genetic Variation; D006168:Guinea Pigs; D051379:Mice; D008969:Molecular Sequence Data; D010325:Pasteurella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D011817:Rabbits; D051381:Rats; D017421:Sequence Analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S