Comparison of embryo implantation in Wistar rats that underwent ovarian stimulation using exogenous gonadotropins associated with cetrorelix acetate or leuprolide acetate.
OBJECTIVE
To compare embryo implantation in Wistar rats submitted to ovarian stimulation using recombinant FSH (rFSH) with cetrorelix acetate or leuprolide acetate.


METHODS
Experimental study.


METHODS
Faculty of medicine animal facility.


METHODS
Fifty-six female Wistar rats with normal estrus cycles and 30 male.


METHODS
Ovarian stimulation and laparotomy (by the day 13 of gestation).


METHODS
Embryo implantation.


RESULTS
The female rats were subdivided into four groups: group 1, medicated with rFSH, hCG, and cetrorelix acetate; group 2, medicated with rFSH, hCG, and leuprolide acetate; group 3, medicated with rFSH and hCG; and group 4, in which only saline solution was administered. The female rats were mated with fertile male rats on the day of hCG administration with copulation confirmed through cytologic vaginal analysis. The females were killed on the 13th day of gestation. After laparotomy, comparison and identification was done regarding the numbers of corpora lutea and embryo implantations and gestation rates. Group 1 presented lower numbers of corpora lutea and embryo implantations in comparison to the other groups (P<.05). A difference was not found in the gestation rates between the groups.


CONCLUSIONS
The number of embryo implantations in Wistar rats medicated with rFSH and cetrorelix acetate is lower than that of rats medicated with rFSH and leuprolide acetate.
Torres
Mychelle de Medeiros Garcia
Mde M
Obstetrics and Gynecology Department, Irmandade da Santa Casa de Misericórdia de São Paulo, São Paulo, Brazil. mytorres@ig.com.br
Donadio
Nilson
N
Donadio
Nilka Fernandes
NF
Brandão
Alessandra Cunha
AC
Heck
Benjamin
B
D000818:Animals; D010064:Embryo Implantation / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D006062:Gonadotropins / Q000494:pharmacology*; D006727:Hormone Antagonists / Q000494:pharmacology*; D016729:Leuprolide / Q000494:pharmacology*; D008297:Male; D010062:Ovulation Induction / Q000379:methods*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D005300:Fertility Agents, Female; D006062:Gonadotropins; D006727:Hormone Antagonists; D007987:Gonadotropin-Releasing Hormone; D016729:Leuprolide; C062876:cetrorelix