[Role of estrogen receptor-related receptors alpha, beta and gamma in ovarian cancer cells].
OBJECTIVE
To investigate the role of human estrogen receptor-related receptors (ERRs) in ovarian cancer.


METHODS
A green fluorescent protein (GFP) reporter plasmid of ERRalpha was constructed and transfected into ovarian cancer cell lines SKOV3 and OVCAR3. RT-PCR was used to analyze the expression of ERRalpha, ERRbeta, and ERRgamma mRNA in 33 primary ovarian cancer samples and 12 normal ovarian epithelial cells. Progression-free survival time and overall survival time of patients with different expression of ERRs were analyzed by Kaplan-Meier method.


RESULTS
GFP-ERRalpha fusion protein showed ERRalpha expressed in the ovarian cancer cell nucleus. Expressions of the ERRalpha and ERRgamma were increased in the ovarian cancers (58% and 48%) compared with the normal ovarian tissues (P < 0.05); ERRbeta was weakly expressed in the ovarian cancer (9%) as well as in the normal tissues (0). Expression of ERRalpha was positively correlated with the International Federation of Gynecology and Obstetrics (FIGO) stage (r = 0.639, P = 0.017) and cell grading (r = 0.520, P = 0.022). Survival analysis showed that ERRalpha positive group had a poorer overall survival (19.0 months vs. 31.5 months, P < 0.05) and ERRgamma positive group had a longer progression-free survival (18.0 months vs. 13.5 months, P < 0.05).


CONCLUSIONS
ERRalpha chiefly expresses in the cell nucleus. ERRalpha and ERRgamma may play important roles in ovarian cancer.
Sun
Peng-ming
PM
Department Of Gynecology, People's Hospital, Peking University, Beijing 100044, China.
Wei
Li-hui
LH
Sehouli
Jalid
J
Denkert
Carsten
C
Zhao
Dan
D
Gao
Min
M
Sun
Xiu-li
XL
Litchtenegger
Werner
W
D047628:Estrogen Receptor alpha / Q000378:metabolism; D005260:Female; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D011379:Prognosis; D011960:Receptors, Estrogen / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097983:ERRalpha Estrogen-Related Receptor
D047628:Estrogen Receptor alpha; D049452:Green Fluorescent Proteins; D011960:Receptors, Estrogen; D000097983:ERRalpha Estrogen-Related Receptor; C506487:ESR1 protein, human; C490917:ESRRG protein, human; C107914:estrogen receptor-related receptor beta