title: Rev1 employs a novel mechanism of DNA synthesis using a protein template.
abstract: The Rev1 DNA polymerase is highly specialized for the incorporation of C opposite template G. We present here the crystal structure of yeast Rev1 bound to template G and incoming 2'-deoxycytidine 5'-triphosphate (dCTP), which reveals that the polymerase itself dictates the identity of the incoming nucleotide, as well as the identity of the templating base. Template G and incoming dCTP do not pair with each other. Instead, the template G is evicted from the DNA helix, and it makes optimal hydrogen bonds with a segment of Rev1. Also, unlike other DNA polymerases, incoming dCTP pairs with an arginine rather than the templating base, which ensures the incorporation of dCTP over other incoming nucleotides. This mechanism provides an elegant means for promoting proficient and error-free synthesis through N2-adducted guanines that obstruct replication.
authors.affiliation: Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, Box 1677, 1425 Madison Avenue, New York, NY 10029, USA.
authors.forename: Deepak T
authors.initials: DT
authors.lastname: Nair
authors.forename: Robert E
authors.initials: RE
authors.lastname: Johnson
authors.forename: Louise
authors.initials: L
authors.lastname: Prakash
authors.forename: Satya
authors.initials: S
authors.lastname: Prakash
authors.forename: Aneel K
authors.initials: AK
authors.lastname: Aggarwal
mesh_terms: D001120:Arginine / Q000378:metabolism; D020029:Base Pairing; D001665:Binding Sites; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis*; D003845:Deoxycytosine Nucleotides / Q000737:chemistry / Q000378:metabolism*; D006147:Guanine / Q000737:chemistry / Q000378:metabolism*; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D009713:Nucleotidyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013698:Templates, Genetic; D000099270:Y-Family DNA Polymerases
chemical_list: D001120:Arginine; D004271:DNA, Fungal; D003845:Deoxycytosine Nucleotides; D006147:Guanine; D009713:Nucleotidyltransferases; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C024107:2'-deoxycytidine 5'-triphosphate; C100951:REV1 protein, S cerevisiae; D000099270:Y-Family DNA Polymerases