[Genotype of isolated strain of hantavirus in Liaoning area].
OBJECTIVE
To isolate hantavirus and to genotype isolated strains of hantavirus (SY 13) in Liaoning province.


METHODS
Hantavirus was isolated by means of cell culture. The G1, G2 fragments of M segments and S segment of the strain of SY 13 were amplified respectively by means of RT-PCR in order to compare and analyze the homology between the sequences of the three isolated gene fragments and the sequences downloaded from GenBank with neighbor-joining through sequence analysis software of DNA Star, Clustal, Phylip, etc.


RESULTS
SY 13 and HTN showed higher homology, reached up to 79.3%-97.0%; SY 13 and BAO 10, BAO 14, JIANG 13, BAO 9, Q 33 were on the same branch and their homology reached up to 95.0%-97.0%.


CONCLUSIONS
The isolated hantavirus strain in Liaoning area was the same as the one in Heilongjiang area which belongs to HTN H4.
Dong
Xue
X
Microbiologcal Test Section, Shenyang Center for Disease Control and Prevention, Shenyang, China.
Zhang
Yong-zhen
YZ
Li
Xin
X
Zhao
Chang-zhi
CZ
Wang
Bing
B
D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D005838:Genotype; D006476:Orthohantavirus / Q000235:genetics* / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000821:virology*; D051379:Mice; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014709:Vero Cells
D012367:RNA, Viral