Induction of long-term depression and phosphorylation of the delta2 glutamate receptor by protein kinase C in cerebellar slices.
The phosphorylation of ionotropic glutamate receptors (iGluRs) by protein kinases plays a crucial role in synaptic plasticity. In the cerebellum, protein kinase C (PKC) activation is required for the induction of long-term depression (LTD) at parallel fibre-Purkinje cell synapses. Although delta2 glutamate receptors (GluRdelta2), expressed predominantly in Purkinje cells, are essential for cerebellar LTD, little is known about the mechanism by which GluRdelta2 participates in LTD or its relationship with PKC activation pathways. We found that a PKC activator, phorbol ester, induced postsynaptic LTD in Purkinje cells in mouse cerebellar slice preparations without significantly changing the presynaptic properties. Under this condition, the GluRdelta2 prepared from the cerebellar slices was significantly phosphorylated. Indeed, the C-terminus of the GluRdelta2 fused with glutathione-S-transferase (GST) was directly phosphorylated by purified PKC at a specific serine residue. In addition, two-dimensional phosphopeptide mapping analysis indicated that the major phosphorylation site of the GST-fusion protein containing the C-terminus of GluRdelta2 was identical to that of GluRdelta2 prepared from cerebellar slices. Therefore, GluRdelta2 is phosphorylated by PKC in vitro and by an LTD-inducing stimulus in slice preparations. Because this region of GluRdelta2 is known to associate with certain intracellular molecules, the PKC phosphorylation status of the C-terminus of GluRdelta2 may be involved in new signaling pathways during LTD.
Kondo
Tetsuro
T
Molecular Neurophysiology, Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan.
Kakegawa
Wataru
W
Yuzaki
Michisuke
M
D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western / Q000379:methods; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology*; D004586:Electrophoresis / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D066298:In Vitro Techniques; D036881:Long-Term Synaptic Depression / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques / Q000379:methods; D010704:Phorbols / Q000494:pharmacology; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism* / Q000502:physiology; D017470:Receptors, Glutamate / Q000378:metabolism*
D010704:Phorbols; D017470:Receptors, Glutamate; C107913:glutamate receptor delta 2; D011493:Protein Kinase C; C033085:phorbol