title: [Association of thymidylate synthase polymorphism with esophageal squamous-cell carcinoma].
abstract: OBJECTIVE
  To explore the influence of 5'UTR tandem repeat and 3'UTR 6 bp deletion polymorphism of thymidylate synthase (TS) gene on the development and lymphatic metastases of esophageal squamous-cell carcinoma (ESCC).
  
  
  METHODS
  Peripheral leucocyte DNA was extracted from 232 ESCC patients and 348 age- and sex-matched healthy controls. TS 5'UTR and 3'UTR genotyping in all study subjects was performed by PCR fragment analysis and PCR-RFLP analysis, respectively.
  
  
  RESULTS
  The distribution of TS 5'UTR and 3'UTR variants in ESCC patients was not significantly different from that in healthy controls. However, individuals with 6 bp+/6 bp+ and 3R/3R genotypes significantly reduced the risk to ESCC development compared to those with other genotype combinations (adjusted OR = 0.32, 95% CI = 0.08-0.92). In addition, the frequency of 2R/3R genotype in ESCC patients with lymphatic metastases (40%) was significantly higher than that in lymph node negative cases (14.7%) (chi(2) = 10.11, P = 0.001). Compared to 3R/3R genotype, the 2R/3R genotype significantly increased the risk of lymphatic metastases in ESCC (adjusted OR = 3.68, 95% CI = 1.54-8.93).
  
  
  CONCLUSIONS
  The genotyping of TS 5'UTR and 3'UTR polymorphisms might be used as a stratification maker for predicting susceptibility to ESCC. The TS 5'UTR 2R/3R genotype might be a candidate molecular marker to predict the potential of lymphatic metastases in ESCC.
authors.affiliation: Heibei Cancer Institute, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China.
authors.forename: Ya-jing
authors.initials: YJ
authors.lastname: Cui
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Deng-gui
authors.initials: DG
authors.lastname: Wen
authors.forename: Li-zeng
authors.initials: LZ
authors.lastname: Wei
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D005838:Genotype; D006801:Humans; D008207:Lymphatic Metastasis; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences / Q000235:genetics; D013940:Thymidylate Synthase / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D013940:Thymidylate Synthase