Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima.
Amphibian skin is a rich resource of bioactive peptides like proline-rich bombesin from frog Bombina maxima. A novel cDNA clone encoding a precursor protein that comprises proline-rich bombesin and a novel peptide, designated as bombestatin, was isolated from a skin cDNA library of B. maxima. The predicted primary structure of the novel peptide is WEVLLNVALIRLELLSCRSSKDQDQKESCGMHSW, in which two cysteines form a disulfide bond. A BLAST search of databases did not detect sequences with significant similarity. Bombestatin possesses dose-dependent contractile activity on rat stomach strips. The differences between cDNAs encoding PR-bombesin plus bombestatin and PR-bombesin alone are due to fragment insertions located in 3'-coding region and 3'-untranslational region, respectively.
Shen
Ji-Hong
JH
Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China.
Liu
Shu-Bai
SB
Zhang
Ying-Xia
YX
Jin
Yang
Y
Lee
Wen-Hui
WH
Zhang
Yun
Y
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001001:Anura / Q000235:genetics*; D001483:Base Sequence; D001839:Bombesin / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D015723:Gene Library; D008297:Male; D008969:Molecular Sequence Data; D011498:Protein Precursors; D012867:Skin / Q000737:chemistry*
D029845:Amphibian Proteins; D018076:DNA, Complementary; D011498:Protein Precursors; C510988:bombestatin, Bombina maxima; D001839:Bombesin