Characterization of a new methylated Beta-cyclodextrin with a low degree of substitution by electrospray ionization mass spectrometry and liquid chromatography/mass spectrometry.
A new methylated beta-cyclodextrin (Me-beta-CD) with a low degree of substitution (DS) was characterized by electrospray ionization mass spectrometry (ESI-MS) and liquid chromatography coupled with ESI-MS (LC/ESI-MS). For ESI-MS analyses, the composition of the infused sample solution was optimized in order to obtain only singly charged ammoniated CDs without fragmentation. The DS value (i.e. the number of methyl groups per glucopyranose unit) was found to be 0.7, which was in accordance with the values previously obtained by other methods. The LC/ESI-MS analysis, derived from a method using evaporative light scattering detection, allowed the study of the substitution isomers of each derivative and appears to be an easy and rapid tool for the accurate characterization of Me-beta-CD mixtures.
Jacquet
Romain
R
Institut de Chimie Organique et Analytique, UMR CNRS 6005, FR 2708, Université d'Orléans, UFR Sciences, BP 6759, rue de Chartres, 45067 Orléans Cedex 2, France.
Elfakir
Claire
C
Lafosse
Michel
M
D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D047392:beta-Cyclodextrins / Q000032:analysis / Q000737:chemistry*
D047392:beta-Cyclodextrins; C031215:betadex