title: Lipopolysaccharide binding protein, cytokine production in whole blood, and lipoproteins in cystic fibrosis.
abstract: According to the endotoxin lipoprotein hypothesis, lipoproteins may down-regulate cytokine production by neutralizing lipopolysaccharide (LPS) binding protein (LBP) complexes. We investigated the correlation between lipoproteins, LBP, cytokine production, and clinical status in Delta F 508 (homozygous) individuals. Cystic fibrosis patients with mild disease were compared with those with more severe disease and age-matched controls. LBP, IL-8, and tumor necrosis factor-alpha, using a chemiluminescent immunometric assay, and fat intake, as well as serum triglycerides, cholesterol, very low density lipoprotein, LDL, and HDL were measured. In more severe disease there was a correlation between maximum expiratory flow at 25% of vital capacity and HDL. To adjust for the influence of colonization with Pseudomonas aeruginosa, those who were colonized with P. aeruginosa were analyzed separately. There was a significant correlation between LBP and forced expiratory volume in 1 s. Lipoproteins may have a modulating effect in more advanced disease and are not influenced by fat intake. LBP correlates those who were colonized with P. aeruginosa (Psa+) with clinical status as well as lung function and may be a critical molecule regulating LPS-induced inflammation.
authors.affiliation: Children's Hospital Medical Center, University of Bonn, 53113 Bonn, Germany. s.schmitt.grohe@uni-bonn.de
authors.forename: Sabina
authors.initials: S
authors.lastname: Schmitt-Grohé
authors.forename: Valerie
authors.initials: V
authors.lastname: Hippe
authors.forename: Michael
authors.initials: M
authors.lastname: Igel
authors.forename: Karl
authors.initials: K
authors.lastname: von Bergmann
authors.forename: Heinz G
authors.initials: HG
authors.lastname: Posselt
authors.forename: Andreas
authors.initials: A
authors.lastname: Krahl
authors.forename: Christina
authors.initials: C
authors.lastname: Smaczny
authors.forename: Thomas O F
authors.initials: TO
authors.lastname: Wagner
authors.forename: Wilfried
authors.initials: W
authors.lastname: Nikolazik
authors.forename: Ralf
authors.initials: R
authors.lastname: Schubert
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Lentze
authors.forename: Stefan
authors.initials: S
authors.lastname: Zielen
mesh_terms: D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000293:Adolescent; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000097:blood; D002648:Child; D003550:Cystic Fibrosis / Q000097:blood* / Q000235:genetics / Q000503:physiopathology; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000097:blood; D009154:Mutation; D012129:Respiratory Function Tests; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D016207:Cytokines; D008074:Lipoproteins; D008562:Membrane Glycoproteins; D000099231:Lipopolysaccharide-Binding Protein