Early effects of modulating nuclear factor-kappaB activation on traumatic spinal cord injury in rats.
Genes regulated by NF-kappaB play an important role on secondary damage and repair after spinal cord injury (SCI). To assess the early effects of the pharmacological inhibition and overactivation of NF-kappaB, pyrrolidine dithiocarbamate (PDTC) or lipopolysaccharide were given to rats before or after SC contusion. The amount of spared SC tissue was higher (P < 0.05) at 24 h postinjury in rats post-treated with PDTC; both PDTC-treated rats showed no significant trend to decrease polymorphonuclear infiltrate. p65 subunit was present in inflammatory cells, neurons, and astrocytes at the injury site. These data support further investigation on functional effects of NF-kappaB inhibition in acute SCI.
Jiménez-Garza
Octavio
O
Pharmacology Section, CINVESTAV-IPN, México City, México.
Camacho
Javier
J
Ibarra
Antonio
A
Martínez
Angelina
A
Guízar-Sahagún
Gabriel
G
D000818:Animals; D001711:Biotransformation / Q000187:drug effects; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000633:toxicity; D016328:NF-kappa B / Q000378:metabolism / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology; D011759:Pyrrolidines / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D013119:Spinal Cord Injuries / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D013859:Thiocarbamates / Q000494:pharmacology / Q000627:therapeutic use
D008070:Lipopolysaccharides; D016328:NF-kappa B; D011759:Pyrrolidines; D013859:Thiocarbamates; C020972:pyrrolidine dithiocarbamic acid