title: Transgenic tobacco plants overexpressing the Met25 gene of Saccharomyces cerevisiae exhibit enhanced levels of cysteine and glutathione and increased tolerance to oxidative stress.
abstract: The cysteine biosynthesis pathway differs between plants and the yeast Saccharomyces cerevisiae. The yeast MET25 gene encoded to O-acetylhomoserine sulfhydrylase (AHS) catalyzed the reaction that form homocysteine, which later can be converted into cystiene. In vitro studies show that this enzyme possesses also the activity of O-acetyl(thiol)lyase (OASTL) that catalyzes synthesis of cysteine in plants. In this study, we generated transgenic tobacco plants expressing the yeast MET25 gene under the control of a constitutive promoter and targeted the yeast protein to the cytosol or to the chloroplasts. Both sets of transgenic plants were taller and greener than wild-type plants. Addition of SO(2), the substrate of the yeast enzyme caused a significant elevation of the glutathione content in representative plants from each of the two sets of transgenic plants expressing the yeast gene. Determination of non-protein thiol content indicated up to four-folds higher cysteine and 2.5-fold glutathione levels in these plants. In addition, the leaf discs of the transgenic plants were more tolerant to toxic levels of sulphite, and to paraquat, an herbicide generating active oxygen species.
authors.affiliation: Plant Science Laboratory, Migal -- Galilee Technology Center, Kiryat Shmona, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Matityahu
authors.forename: L
authors.initials: L
authors.lastname: Kachan
authors.forename: I
authors.initials: I
authors.lastname: Bar Ilan
authors.forename: R
authors.initials: R
authors.lastname: Amir
mesh_terms: D000222:Adaptation, Physiological; D002734:Chlorophyll / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism*; D003547:Cysteine Synthase; D015870:Gene Expression; D005816:Genetic Complementation Test; D005978:Glutathione / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics*; D018384:Oxidative Stress; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D012995:Solubility; D013431:Sulfates / Q000378:metabolism; D013447:Sulfites / Q000494:pharmacology; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D029701:Saccharomyces cerevisiae Proteins; D013431:Sulfates; D013447:Sulfites; D002734:Chlorophyll; D008715:Methionine; D003547:Cysteine Synthase; C103381:MET17 protein, S cerevisiae; D005978:Glutathione; D003545:Cysteine