Preparative isolation and purification of polymethoxylated flavones from Tangerine peel using high-speed counter-current chromatography.
Several polymethoxylated flavones including nobiletin, 3,5,6,7,8,3',4'-heptamethoxyflavone, tangeretin and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone were separated from Tangerine peel (Juhong in Chinese) by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.8:1:1, v/v). Then, 26 mg of nobiletin, 6 mg of 3,5,6,7,8,3',4'-heptamethoxyflavone, 35 mg of tangeretin and 11 mg of 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone could be obtained from 150 mg crude extracts and their purities were 98.6%, 95.9%, 99.8% and 96.8%, respectively. All these constituents were identified by EI-MS and 1H NMR.
Wang
Xiao
X
Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China. wangx@keylab.net
Li
Fuwei
F
Zhang
Hongxia
H
Geng
Yanling
Y
Yuan
Jingpeng
J
Jiang
Ting
T
D002957:Citrus / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods*; D047309:Flavones / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D013056:Spectrophotometry, Ultraviolet
D047309:Flavones