Advantages of multi-color fluorescent proteins for whole-body and in vivo cellular imaging.
The revolution of in vivo cancer biology enabled by fluorescent proteins is described. The high extinction coefficients, quantum yields, and unique spectral properties of fluorescent proteins have been taken advantage of in order to visualize, in real time, the important aspects of cancer in living animals, including tumor cell trafficking, invasion, metastasis, and angiogenesis. Fluorescent proteins enable whole-body imaging of tumors on internal organs. These multicolored proteins have allowed the color-coding of cancer cells growing in vivo with distinction of different cell types, including host from tumor, with single-cell resolution.
Hoffman
Robert M
RM
AntiCancer, Inc, 7917 Ostrow Street, San Diego, California 92111, USA. all@anticancer.com
D000818:Animals; D005455:Fluorescent Antibody Technique / Q000379:methods; D005456:Fluorescent Dyes; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D008164:Luminescent Proteins; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008967:Molecular Biology / Q000379:methods; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D013673:Technology Assessment, Biomedical; D051598:Whole Body Imaging / Q000379:methods*
D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D009363:Neoplasm Proteins