Optimization of conditions for supercritical fluid extraction of flavonoids from hops (Humulus lupulus L.).
Waste hops are good sources of flavonoids. Extraction of flavonoids from waste hops (SC-CO(2) extracted hops) using supercritical fluids technology was investigated. Various temperatures, pressures and concentrations of ethanol (modifier) and the ratio (w/w) of solvent to material were tested in this study. The results of single factor and orthogonal experiments showed that at 50 degrees C, 25 MPa, the ratio of solvent to material (50%), ethanol concentration (80%) resulted in maximum extraction yield flavonoids (7.8 mg/g). HPLC-MS analysis of the extracts indicated that flavonoids obtained were xanthohumol, the principal prenylflavonoid in hops.
He
Guo-Qing
GQ
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China. gqhe@zju.edu.cn
Xiong
Hao-Ping
HP
Chen
Qi-He
QH
Ruan
Hui
H
Wang
Zhao-Yue
ZY
Traoré
Lonseny
L
D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification*; D027582:Humulus / Q000737:chemistry*; D013058:Mass Spectrometry
D005419:Flavonoids; D000431:Ethanol