Proteome analysis to assess physiological changes in Escherichia coli grown under glucose-limited fed-batch conditions.
Proteome analysis was used to compare global protein expression changes in Escherichia coli fermentation between exponential and glucose-limited fed-batch phase. Two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry were used to separate and identify 49 proteins showing >2-fold difference in expression. Proteins upregulated during exponential phase include ribonucleotide biosynthesis enzymes and ribosomal recycling factor. Proteins upregulated during fed-batch phase include those involved in high-affinity glucose uptake, transport and degradation of alternate carbon sources and TCA cycle, suggesting an enhanced role of the cycle under glucose- and energy-limited conditions. We report the upregulation of several putative proteins (ytfQ, ygiS, ynaF, yggX, yfeX), not identified in any previous study under carbon-limited conditions.
Raman
Babu
B
Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County (UMBC), ECS 314, 1000 Hilltop Circle, Baltimore, Maryland 21250, USA.
Nandakumar
M P
MP
Muthuvijayan
Vignesh
V
Marten
Mark R
MR
D000222:Adaptation, Physiological / Q000502:physiology; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005947:Glucose / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*
D029968:Escherichia coli Proteins; D020543:Proteome; D005947:Glucose