Experimental transmission of West Nile virus (Flaviviridae: Flavivirus) by Carios capensis ticks from North America.
Seabird soft ticks, Carios capensis (Ixodida: Argasidae), originally collected from coastal Georgia, USA, were allowed to ingest a blood meal from pekin ducklings (Anas domesticus) infected with WNV. After 35 days of extrinsic incubation, the ticks transmitted virus to naive ducklings. WNV was detected via plaque assay and RTPCR in ticks and in tissues and serum of ducklings 7 days post infestation.
Hutcheson
H Joel
HJ
Department of Bioagricultural Sciences and Pest Management, Arthropod-borne and Infectious Diseases Laboratory (AIDL), Colorado State University (CSU), Fort Collins, Colorado 80523-1692, USA. joel.hutcheson@colostate.edu
Gorham
Christopher H
CH
Machain-Williams
Carlos
C
Loroño-Pino
Maria A
MA
James
Angela M
AM
Marlenee
Nicole L
NL
Winn
Bradford
B
Beaty
Barry J
BJ
Blair
Carol D
CD
D000818:Animals; D001096:Arachnid Vectors / Q000821:virology*; D001715:Bird Diseases / Q000469:parasitology / Q000635:transmission*; D004372:Ducks / Q000469:parasitology; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013987:Ticks / Q000821:virology*; D014766:Viremia / Q000662:veterinary; D014901:West Nile Fever / Q000469:parasitology / Q000635:transmission*; D014902:West Nile virus / Q000302:isolation & purification*
D012367:RNA, Viral