title: Periodontal growth factors and tissue carriers: biocompatibility and mitogenic efficacy in vitro.
abstract: Clinical research has long been testing techniques of integrating biomaterials with many external factors, such as simple proteins or more complicated devices, in order to achieve the restitutio ad integrum of periodontium. This study assessed the in vitro effectiveness of platelet derivate growth factor-BB (PDGF) and insulin growth factor I (IGF); the biocompatibility of materials like Paroguide, Oclastim membranes, Gingistat sponges, Surgiplaster, and Capset; and their efficacy as carriers for the platelet derivate growth factor-BB (PDGF) and insulin growth factor I (IGF). Fibroblasts from the human periodontal ligament were incubated with growth factors free or vehiculated. Mitogenic effect was evaluated by measuring the growth rate and biocompatibility by observing cell morphology at SEM. PDGF was the most effective in stimulating cell proliferation both in solution (p < 0.001) and vehiculated (p < 0.01). Surgiplaster and Capset were more biocompatible; however, final analysis to assess their efficacy as carriers failed to disclose significant differences between experimental findings and control.
authors.affiliation: Department of Periodontology, University of Bologna, Via San Vitale 59, 40125 Bologna, Italy. claudio.cesari@fastwebnet.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Cesari
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Gatto
authors.forename: Francesco
authors.initials: F
authors.lastname: Malucelli
authors.forename: Mario
authors.initials: M
authors.lastname: Raspanti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Zucchelli
authors.forename: Luigi
authors.initials: L
authors.lastname: Checchi
mesh_terms: D000293:Adolescent; D000328:Adult; D001672:Biocompatible Materials; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D005881:Gingiva / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D008934:Mitogens / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D008934:Mitogens; D010982:Platelet-Derived Growth Factor; D007334:Insulin-Like Growth Factor I