title: [Establishment and application of fluorescent quantitative PCR method for detecting human herpes virus type 6].
abstract: OBJECTIVE
  To establish fluorescent quantitative PCR method for detecting human herpes virus type 6 (HHV6).
  
  
  METHODS
  According to the specific sequence of human herpes virus type 6 genes, the primers and the fluorescent probe (TaqMan) were designed and synthesized. The fragment generated from HHV 6 gene as template was cloned into the pMD18-T vector which was constructed from the pUC 18. And the positive recombinant plasmid was 1:10 diluted and used as standard quantitative template to make the standard curve for sample detection.
  
  
  RESULTS
  The standard curve indicated the linear relationship between Ct (cycle threshold) and template concentration. The clinical samples from 135 cases were detected by this system, 16 cases among 135 were positive.
  
  
  CONCLUSIONS
  The fluorescent quantitative PCR method for the detection of human herpes virus type 6 is simple and accurate, and this method may be helpful to clinical diagnosis.
authors.affiliation: Central Laboratory, Shaoxing People's Hospital, Shaoxing, China.
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Dong
authors.forename: Chang-gen
authors.initials: CG
authors.lastname: Shi
authors.forename: Shi-wen
authors.initials: SW
authors.lastname: Qu
authors.forename: Zhong-min
authors.initials: ZM
authors.lastname: Liu
authors.forename: Jia-ping
authors.initials: JP
authors.lastname: Fu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015654:Herpesvirus 6, Human / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000175:diagnosis / Q000821:virology*; D015203:Reproducibility of Results; D019349:Roseolovirus Infections / Q000175:diagnosis / Q000821:virology*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral