title: Lack of an association between candidate gene loci and idiopathic generalized epilepsy in Kuwaiti Arab children.
abstract: Idiopathic generalized epilepsies (IGEs) are the most common types of epilepsy in childhood and adolescence. A variety of data suggest that IGEs have a predominant genetic etiology. Recently, a number of gene mutations have been found to be associated with various types of epilepsy in mainly the Caucasian populations. The objective of this study was to investigate the association of three different candidate genes with IGE in Kuwaiti Arab children. This study includes 123 Kuwaiti patients with a confirmed diagnosis of epilepsy. Most of the patients have had a diagnostic EEG with generalized spike-wave discharges (GSWs). All patients were evaluated by using a validated seizure questionnaire. The clinical type of epilepsy was determined by a trained neurologist/pediatrician. The study also include 100 controls, the control subjects were children which did not have any history of neurological disorders. Blood samples were collected from all patients and control subjects after taking informed consent. DNA was isolated and analyzed by molecular methods. A FokI polymorphism in neuronal nicotinic acetylcholine receptor alpha-4 subunit (CHRNA4) gene was detected by PCR-RFLP method. A missense mutation (Ser248Phe) in CHRNA4 gene was analyzed by PCR-RFLP using HpaII. A C121W mutation in sodium-channel beta-1 subunit (SCN1B) gene was screened by a PCR-RFLP method using HinPI. A 2-bp deletion in Cystatin B gene was detected by PCR-RFLP using XcmI. The incidence of three FokI polymorphism genotypes in Kuwaiti IGE patients was 1,1 (85%), 1,2 (14%) and 2,2 (1%) respectively. The missense mutation Ser248Phe of CHRNA4 gene was not detected at all in Kuwaiti IGE patients. The C387G transversion resulting in C121W change in third exon of the SCN1B gene was detected in 3/123 patients (2%). The patients carrying this mutation also exhibited febrile seizures. The incidence of 2 bp deletion in the cystatin B gene was found to be 4% (5/123 IGE patients). The data obtained from molecular analysis show a lack of association between three candidate genes and clinical expression of IGE in Kuwaiti Arab children. This is completely different from the findings reported from Caucasian populations of France, Australia and USA in which case a strong association has been reported between IGE and these genes.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Kuwait University, PO Box 24923, Safat, 13110, Kuwait. haider@hsc.edu.kw
authors.forename: M Z
authors.initials: MZ
authors.lastname: Haider
authors.forename: Y
authors.initials: Y
authors.lastname: Habeeb
authors.forename: E
authors.initials: E
authors.lastname: Al-Nakkas
authors.forename: H
authors.initials: H
authors.lastname: Al-Anzi
authors.forename: M
authors.initials: M
authors.lastname: Zaki
authors.forename: A
authors.initials: A
authors.lastname: Al-Tawari
authors.forename: M
authors.initials: M
authors.lastname: Al-Bloushi
mesh_terms: D018912:Arabs; D002648:Child; D055313:Cystatin B; D015891:Cystatins / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D004569:Electroencephalography; D004827:Epilepsy / Q000208:ethnology / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D007730:Kuwait; D009154:Mutation; D011110:Polymorphism, Genetic; D015222:Sodium Channels / Q000235:genetics; D062547:Voltage-Gated Sodium Channel beta-1 Subunit
chemical_list: C524994:CSTB protein, human; D015891:Cystatins; C470379:SCN1B protein, human; D015222:Sodium Channels; D062547:Voltage-Gated Sodium Channel beta-1 Subunit; D055313:Cystatin B; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific