Increased mesquite gum formation in nodal explants cultures after treatment with a microbial biomass preparation.
Prosopis laevigata nodal explants cultures were established in Murashige and Skoog medium. Simultaneously these cultures were subjected to stress with biotic elicitors and an environmental factor (temperature increase to promote heat stress) in order to promote and increase exuded mesquite gum production. The biotic elicitors were: Aspergillus nidulans and Pseudomonas pseudoalcaligenes both used in concentrations of 10, 20 and 30 mg, whereas the environmental condition was different incubation temperatures (25, 35 and 40 degrees C). The greatest gum production (approximately 13 mg of pooled gum from 100 explants after 14 days incubation) took place when the culture medium was added 10, 20 and 30 mg of autoclaved fungal mycelium of A. nidulans or 30 mg of autoclaved bacterial biomass of P. pseudoalcaligenes in combination with an incubation temperature of 35 degrees C. These treatments were non-significantly different among themselves (P < 0.05), but were significantly different to the rest of the treatments (P > 0.05).
Orozco-Villafuerte
Juan
J
Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, 09340 México, DF, Mexico.
Buendía-González
Leticia
L
Cruz-Sosa
Francisco
F
Vernon-Carter
Eduardo J
EJ
D001233:Aspergillus nidulans / Q000502:physiology; D018533:Biomass; D006358:Hot Temperature; D010936:Plant Extracts / Q000096:biosynthesis*; D053149:Plant Gums; D029862:Prosopis / Q000378:metabolism* / Q000382:microbiology*; D044205:Pseudomonas pseudoalcaligenes / Q000502:physiology; D046509:Tissue Culture Techniques
D010936:Plant Extracts; D053149:Plant Gums; C416517:prosopis gum