title: Fructose ingestion enhances atherosclerosis and deposition of advanced glycated end-products in cholesterol-fed rabbits.
abstract: This study was performed to investigate whether the plasma concentration of phosphatidylcholine hydroperoxide (PCOOH), which is a marker of oxidized stress in the blood, increased in cholesterol-fed rabbits, and fructose ingestion promoted this process and aggravated atherosclerosis. Male Japanese white rabbits (age: 12 weeks, and body weight: around 2.0 kg, n = 15) were divided into three groups, (1) a NN group as a normal control fed a standard diet (n = 5), (2) a CN group fed 1.0% cholesterol, and (3) a CF group given both 1.0% cholesterol and 10% fructose-containing tap water. During 8 weeks, plasma PCOOH levels increased significantly in the CN and CF groups compared to the NN group and fructose further raised the PCOOH level. The atherosclerosis was significantly promoted and the deposition of advanced glycation end products (AGEs) was marked in the CF group compared to the CN group. Fructose worsened the atheromatous lesions caused by cholesterol feeding. The mechanism is most likely through lipid peroxidation, which was increased by cholesterol feeding-induced hyperlipidemia, and the formation of AGEs.
authors.affiliation: Division of Molecular Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Tokita
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Hirayama
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sekikawa
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kotake
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Toyota
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Sawai
authors.forename: Shinichi
authors.initials: S
authors.lastname: Oikawa
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000139:chemically induced*; D002784:Cholesterol / Q000008:administration & dosage / Q000494:pharmacology*; D005632:Fructose / Q000008:administration & dosage / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006949:Hyperlipidemias; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D010713:Phosphatidylcholines / Q000097:blood; D011817:Rabbits
chemical_list: D017127:Glycation End Products, Advanced; D010713:Phosphatidylcholines; C057590:phosphatidylcholine hydroperoxide; D005632:Fructose; D002784:Cholesterol