title: Use of CYP2E1-transfected human liver cell lines in elucidating the actions of ethanol.
abstract: This article represents the proceedings of a symposium at the 2004 RSA Meeting held in Vancouver, Canada. The chairs were Arthur I. Cederbaum and Raj Lakshman. The presentations were (1) ethanol regulates 2,6-sialyltransferase (2,6-ST) gene expression posttranscriptionally by the interaction of a cytosolic binding protein with 2,6-ST mRNA in CYP2E1- and ADH-transfected HepG2 cells, by Raj Lakshman; (2) nature versus nurture: HepG2-E47 cells as a tool to investigate mechanisms of ethanol-mediated potentiation of cell killing, by Jan B. Hoek; (3) ethanol up-regulates profibrogenic connective tissue growth factor gene expression in HepG2 cells via cytochrome P-450 2E1-mediated ethanol oxidation, by Masahiro Konishi; (4) role of calcium and calcium-activated enzymes in CYP2E1-dependent toxicity, by Arthur I Cederbaum; (5) the use of cell lines to characterize the role of CYP2E1 in the metabolism of farnesol, by Dennis Koop; and (6) studies with HepG2 cells that express the two major ethanol-metabolizing enzymes, by Terrence M. Donohue.
authors.affiliation: Lipid Research Laboratory, VA Medical Center, and the Department of Biochemistry, Molecular Biology, and Medicine, George Washington University, Washington, DC 20422, USA. raj.lakshman@med.va.gov
authors.forename: Raj
authors.initials: R
authors.lastname: Lakshman
authors.forename: Arthur I
authors.initials: AI
authors.lastname: Cederbaum
authors.forename: Jan B
authors.initials: JB
authors.lastname: Hoek
authors.forename: Masahiro
authors.initials: M
authors.lastname: Konishi
authors.forename: Dennis
authors.initials: D
authors.lastname: Koop
authors.forename: Terrence M
authors.initials: TM
authors.lastname: Donohu
mesh_terms: D000426:Alcohol Dehydrogenase / Q000502:physiology; D002118:Calcium / Q000502:physiology; D002460:Cell Line; D055513:Connective Tissue Growth Factor; D019392:Cytochrome P-450 CYP2E1 / Q000502:physiology*; D000431:Ethanol / Q000378:metabolism / Q000633:toxicity*; D005204:Farnesol / Q000378:metabolism; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D008099:Liver / Q000187:drug effects*; D010084:Oxidation-Reduction; D012799:Sialyltransferases / Q000235:genetics; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000502:physiology
chemical_list: C526317:CCN2 protein, human; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D014409:Tumor Necrosis Factor-alpha; D055513:Connective Tissue Growth Factor; D000431:Ethanol; D005204:Farnesol; D000426:Alcohol Dehydrogenase; D019392:Cytochrome P-450 CYP2E1; D012799:Sialyltransferases; D048051:p38 Mitogen-Activated Protein Kinases; D002118:Calcium