Synthesis and hybridization studies of DNA on functionalized polypropylene surfaces.
Polypropylene (PP) slice was treated with plasma in a mixture of nitrogen and hydrogen (1:2 V/V). Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) demonstrated the successful grafting of amino groups. The as-treated slices were successively applied to the in-situ synthesis of oligonucleotides and an average coupling yield of more than 98% was achieved. The hybridization signals were recorded with fluorescent analysis system. The complementary and mismatched sequences were distinguished clearly, and the ratio of fluorescence intensity values (arbitrary units) for perfect match:single base mismatch:two bases mismatch:three bases mismatch is 108.6: 62.9: 22.4: 5.5. The results implied the plasmamodified PP surface was extremely stable, performed well in DNA hybridization assays, and could service as a good substrate for high-density oligonucleotide array synthesis, especially in a multistep molecular stamping method to fabricate DNA microarrays in-situ on a large scale.
Tang
Jianxin
J
Laboratory of Nano-Biomedicines and Devices, Zhuzhou Institute of Technology, Zhuzhou 412008, P R. China.
He
Quanguo
Q
Chen
Hong
H
He
Nongyue
N
D015342:DNA Probes / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics; D006801:Humans; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D011126:Polypropylenes; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis; D013499:Surface Properties
D015342:DNA Probes; D011126:Polypropylenes