Properties of growth and molecular profiles of rat progenitor cells from ciliary epithelium.
Recent studies have demonstrated that multipotent retinal stem or progenitor cells can be isolated from the ciliary epithelium (CE) of the eye using a neurosphere culture. In this study, we investigated the properties of growth and differentiation, and molecular profiles of rat adult ciliary epithelium (CE)-derived retinal progenitors and forebrain (FB) derived neurospheres. Under clonogenic culture conditions, we found that the CE-derived neurospheres contained fewer undifferentiated cells compared with the FB-derived neurospheres, and that CE-derived neurospheres initially expressed the set of Notch pathway molecules genes including Notch 1 and Delta 1, HES-1 and HES-5, but partially lose their expression after passaging. Furthermore, we found that the CE-derived neurospheres did not express several markers for in vivo embryonic retinal progenitors. Additionally, when the eye was divided into four subregions along its dorsoventral and nasotemporal axes and progenitor cells were obtained from the subregions, the progenitor cells did not express the subregion specific transcription factors, suggesting that subregional specificity is not maintained in vitro. Together, our results demonstrate that CE-derived progenitor cells may have intrinsic limitations in the production of cell types.
Yanagi
Yasuo
Y
Department of Regenerative Medicine, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan. yanagi-tky@umin.ac.jp
Inoue
Yuji
Y
Kawase
Yoko
Y
Uchida
Saiko
S
Tamaki
Yasuhiro
Y
Araie
Makoto
M
Okochi
Hitoshi
H
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000032:analysis / Q000235:genetics; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D002924:Ciliary Body / Q000166:cytology* / Q000378:metabolism; D002999:Clone Cells; D019938:Cyclin D1 / Q000032:analysis / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000032:analysis / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis / Q000235:genetics; D018613:Microscopy, Confocal; D016548:Prosencephalon / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D051881:Receptor, Notch1 / Q000032:analysis / Q000235:genetics; D012097:Repressor Proteins / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D000072056:Transcription Factor HES-1; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics
D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers; D019938:Cyclin D1; D018398:Homeodomain Proteins; D047908:Intracellular Signaling Peptides and Proteins; D051881:Receptor, Notch1; D012097:Repressor Proteins; D000072056:Transcription Factor HES-1; D014157:Transcription Factors; C489735:Hes1 protein, rat; C489686:Hes5 protein, rat