title: The crystal structures of 3-TAPAP in complexes with the urokinase-type plasminogen activator and picrate.
abstract: The urokinase-type plasminogen activator (uPA) is a protein involved in tissue remodeling and other biological processes. The inhibitors of uPA have been shown to prevent the spread of metastasis and tumor growth, and accordingly this enzyme is widely accepted as a promising anticancer target. In this work, we have investigated the conformation of the uPA inhibitor 3-TAPAP in two different crystalline environments of a picrate and a uPA complex. These structures were compared to the known structure of the 3-TAPAP in the complex with trypsin. In the complexes with the proteins, trypsin, and uPA, the binding mode of 3-TAPAP is similar. A larger difference in the conformation, in the comparison to these structures, has been observed by us in the 3-TAPAP picrate crystal. This observation contradicts the hypothesis that 3-TAPAP derivatives inhibit serine proteinases in preformed stable conformations.
authors.affiliation: Department of Chemistry, Pedagogical University, ul. Podchorazych 2, 30-084 Kraków, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Zesławska
authors.forename: Uwe
authors.initials: U
authors.lastname: Jacob
authors.forename: Jörg
authors.initials: J
authors.lastname: Stürzebecher
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Oleksyn
mesh_terms: D000578:Amidines / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001665:Binding Sites; D018360:Crystallography, X-Ray; D018450:Disease Progression; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D006860:Hydrogen Bonding; D008956:Models, Chemical; D008958:Models, Molecular; D008968:Molecular Conformation; D009362:Neoplasm Metastasis; D010851:Picrates / Q000737:chemistry*; D010880:Piperidines / Q000737:chemistry*; D011487:Protein Conformation; D012697:Serine Endopeptidases / Q000737:chemistry; D013237:Stereoisomerism; D014568:Urokinase-Type Plasminogen Activator / Q000737:chemistry*
chemical_list: D000578:Amidines; D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D010851:Picrates; D010880:Piperidines; C070566:N(alpha)-(4-toluenesulfonyl)-3-amidinophenylalanylpiperidine; C005858:picric acid; D012697:Serine Endopeptidases; D014568:Urokinase-Type Plasminogen Activator