title: Effect of ingestion of cow's milk protein hydrolysate formulas on alpha-casein-specific immunoglobulin E and G1 antibody responses in naïve and sensitized mice.
abstract: OBJECTIVE
  Cow's milk protein hydrolysate formulas are widely used for genetically predisposed atopic infants. Whether hydrolysate formulas can induce oral tolerance to alpha-casein was studied for the first time in naive and sensitized mice.
  
  
  METHODS
  Using immunoblotting, residual antigenicity to alpha-casein was examined for in animals fed hydrolysate formulas. Naïve mice fed hydrolysate formulas for 1 to 4 weeks were later sensitized with alpha-casein. Another group of mice sensitized first with alpha-casein were then fed hydrolysate formulas continually for 12 weeks.
  
  
  RESULTS
  Oral tolerance measured by immunoglobulin (Ig)E and IgG1 antibody responses to alpha-casein was induced in naïve mice fed NAN for 1 week or NAN-HA for 4 weeks. IgE responses to alpha-casein were suppressed in mice fed NAN-HA for 1 week or Neoangelac FL for 4 weeks. In contrast, mice fed Alfare, Pepti-Junior, or Pregestimil for 1 to 4 weeks did not develop tolerance to alpha-casein. Antibody responses to alpha-casein were not significantly suppressed in sensitized mice fed NAN or hydrolysate formulas for 12 weeks.
  
  
  CONCLUSIONS
  Primary IgE responses to alpha-casein are readily suppressed in naïve mice first fed cow's milk formula or partially hydrolyzed formula for 1 week. Conversely, ongoing IgE, IgG1, and IgG antibody responses to alpha-casein are poorly suppressed in previously sensitized mice even after prolonged feeding of cow's milk formula or hydrolysate formulas.
authors.affiliation: Department of Pediatrics, Taipei Veterans General Hospital, Taiwan. hjpeng@vghtpe.gov.tw
authors.forename: Ho-Jen
authors.initials: HJ
authors.lastname: Peng
authors.forename: Song-Nan
authors.initials: SN
authors.lastname: Su
authors.forename: Keh-Gong
authors.initials: KG
authors.lastname: Wu
authors.forename: Chi-Kuan
authors.initials: CK
authors.lastname: Ho
authors.forename: Hsiang-Lan
authors.initials: HL
authors.lastname: Kuo
mesh_terms: D000818:Animals; D002364:Caseins / Q000276:immunology; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007223:Infant; D007225:Infant Food; D041943:Infant Formula; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D016269:Milk Hypersensitivity / Q000276:immunology / Q000517:prevention & control*; D008894:Milk Proteins / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002364:Caseins; D007074:Immunoglobulin G; D008894:Milk Proteins; D007073:Immunoglobulin E