Glucose deprivation mediates interaction between CTDK-I and Snf1 in Saccharomyces cerevisiae.
Ctk1 is a kinase involved in transcriptional control. We show in the two-hybrid system that Ctk1 interacts with Snf1, a kinase regulating glucose-dependent genes. Co-purification experiments confirmed the two-hybrid interaction but only when cells were grown at low glucose concentrations. Deletion of Ctk1 or its associated partners, Ctk2 and Ctk3, conferred synthetic lethality with null mutants of Snf1 or Snf1-associated proteins. Northern blot analysis suggested that Ctk1 and Snf1 act together in vivo to regulate GSY2. These findings support the view that Ctk1 interacts with Snf1 in a functional module involved in the cellular response to glucose limitation.
Van Driessche
Benoit
B
Unité de Recherche en Biologie Moléculaire, University of Namur, Belgium. benoit.vandriessche@fundp.ac.be
Coddens
Séverine
S
Van Mullem
Vincent
V
Vandenhaute
Jean
J
D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism*; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014158:Transcription, Genetic
D011494:Protein Kinases; C071570:SNF1-related protein kinases; C060137:carboxy-terminal domain kinase; D017346:Protein Serine-Threonine Kinases; D005947:Glucose