Polymer-coated hollow-fiber microextraction of estrogens in water samples with analysis by gas chromatography-mass spectrometry.
A novel sorbent, dihydroxylated polymethylmethacrylate (DHPMM), coated on hollow-fiber membrane, is used for the polymer-coated hollow-fiber microextraction of trace amounts of natural and synthetic estrogens, such as diethylstilbestrol, estrone, 17beta-estradiol and 17alpha-ethynylestradiol, in aqueous samples. In this procedure, estrogens were extracted using the functionalized polar DHPMM polymer with derivatization using N-methyl-N-(trimethylsilyl)trifluoroacetamide followed by gas chromatography-mass spectrometric analysis. The detection limits for estrogens in aqueous sample were between 0.03 and 0.8 ng l(-1) and the calibration curves were linear over the concentration range 0.05-10 microgl(-1) and had correlation coefficients of >0.994. The relative standard deviations (RSDs) were <15% (n = 3). This simple, accurate, sensitive and selective analytical method is applicable to the determination of trace amounts of estrogens in reservoir and potable water samples.
Basheer
Chanbasha
C
Department of Chemistry, National University of Singapore, Singapore.
Jayaraman
Akhila
A
Kee
Meng Keow
MK
Valiyaveettil
Suresh
S
Lee
Hian Kee
HK
D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D052244:Endocrine Disruptors / Q000302:isolation & purification*; D004967:Estrogens / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply / Q000032:analysis
D052244:Endocrine Disruptors; D004967:Estrogens; D014874:Water Pollutants, Chemical; D019904:Polymethyl Methacrylate