title: Identification of triterpenoid compounds of Centella asiatica by thin-layer chromatography and mass spectrometry.
abstract: The identification of the four principal triterpenoid components of Centella asiatica has been achieved by TLC on silica gel plates and mass spectrometry, as a modification of the method described in the European Pharmacopoeia (5th edn). A combination of ethyl acetate and methanol as the mobile phase was found to be successful in separating these compounds from the rest of the main components of the extract. The spots were detected with anisaldehyde solution. The separated compounds were confirmed by MALDI -TOF mass spectrometry.
authors.affiliation: Laboratorio de Fisiología vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028 Barcelona, Spain. mbonfill@ub.edu
authors.forename: Mercè
authors.initials: M
authors.lastname: Bonfill
authors.forename: Susana
authors.initials: S
authors.lastname: Mangas
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Cusidó
authors.forename: Lidia
authors.initials: L
authors.lastname: Osuna
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Piñol
authors.forename: Javier
authors.initials: J
authors.lastname: Palazón
mesh_terms: D028041:Centella / Q000737:chemistry*; D002855:Chromatography, Thin Layer / Q000379:methods*; D013058:Mass Spectrometry; D053978:Pentacyclic Triterpenes; D013729:Terpenes / Q000302:isolation & purification; D014315:Triterpenes / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D053978:Pentacyclic Triterpenes; D013729:Terpenes; D014315:Triterpenes; C017032:asiatic acid; C093443:madecassoside; C001669:madecassic acid; C004446:asiaticoside