title: Evaluating cutaneous photoaging by use of multiphoton fluorescence and second-harmonic generation microscopy.
abstract: The photoaging process of facial skin is investigated by use of multiphoton fluorescence and second-harmonic generation (SHG) microscopy. We obtain the autofluorescence (AF) and SHG images of the superficial dermis from the facial skin of three patients aged 20, 40, and 70 years. The results show that areas of AF increase with age, whereas areas of SHG decrease with age. The results are consistent with the histological findings in which collagen is progressively replaced by elastic fibers. The AF and SHG changes in photoaging are quantified by a SHG to autofluorescence aging index of dermis (SAAID). Our results suggest that SAAID can be a good indicator of the severity of photoaging.
authors.affiliation: Dermatology, Taipei Campus and Yun-Lin Branch, National Taiwan University Hospital, Taiwan, Taipei.
authors.forename: Sung-Jan
authors.initials: SJ
authors.lastname: Lin
authors.forename: Ruei-
authors.initials: R
authors.lastname: Wu
authors.forename: Hsin-Yuan
authors.initials: HY
authors.lastname: Tan
authors.forename: Wen
authors.initials: W
authors.lastname: Lo
authors.forename: Wei-Chou
authors.initials: WC
authors.lastname: Lin
authors.forename: Tai-Horng
authors.initials: TH
authors.lastname: Young
authors.forename: Chih-Jung
authors.initials: CJ
authors.lastname: Hsu
authors.forename: Jau-Shiuh
authors.initials: JS
authors.lastname: Chen
authors.forename: Shiou-Hwa
authors.initials: SH
authors.lastname: Jee
authors.forename: Chen-Yuan
authors.initials: CY
authors.lastname: Dong
mesh_terms: D000328:Adult; D000368:Aged; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D015595:Skin Aging / Q000473:pathology* / Q000502:physiology*