Programmable assembly of nanoarchitectures using genetically engineered viruses.
Biological systems possess inherent molecular recognition and self-assembly capabilities and are attractive templates for constructing complex material structures with molecular precision. Here we report the assembly of various nanoachitectures including nanoparticle arrays, hetero-nanoparticle architectures, and nanowires utilizing highly engineered M13 bacteriophage as templates. The genome of M13 phage can be rationally engineered to produce viral particles with distinct substrate-specific peptides expressed on the filamentous capsid and the ends, providing a generic template for programmable assembly of complex nanostructures. Phage clones with gold-binding motifs on the capsid and streptavidin-binding motifs at one end are created and used to assemble Au and CdSe nanocrytals into ordered one-dimensional arrays and more complex geometries. Initial studies show such nanoparticle arrays can further function as templates to nucleate highly conductive nanowires that are important for addressing/interconnecting individual nanostructures.
Huang
Yu
Y
Department of Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Chiang
Chung-Yi
CY
Lee
Soo Kwan
SK
Gao
Yan
Y
Hu
Evelyn L
EL
De Yoreo
James
J
Belcher
Angela M
AM
D017104:Bacteriophage M13 / Q000737:chemistry* / Q000235:genetics*; D003460:Crystallization / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics*
D014764:Viral Proteins