Effects of morin on snake venom phospholipase A2 (PLA2).
Flavonoids are potent anti-inflammatory compounds isolated from several plant extracts, and have been used experimentally against inflammatory processes. In this work, a PLA2 isolated from the Crotalus durissus cascavella venom and rat paw oedema were used as a model to study the effect of flavonoids on PLA2. We observed that a treatment of PLA2 with morin induces several modifications in the aromatic amino acids, with accompanying changes in its amino acid composition. In addition, results from circular dichroism spectroscopy and UV scanning revealed important structural modifications. Concomitantly, a considerable decrease in the enzymatic and antibacterial activities was observed, even though anti-inflammatory and neurotoxic activities were not affected. These apparent controversial results may be an indication that PLA2 possess a second pharmacological site which does not affect or depend on the enzymatic activity.
Iglesias
Carolina Veras
CV
Departamento de Bioquímica, Instituto de Biologia, Campinas, São Paulo, Brazil.
Aparicio
Ricardo
R
Rodrigues-Simioni
Léa
L
Camargo
Enilton A
EA
Antunes
Edson
E
Marangoni
Sergio
S
de Oliveira Toyama
Daniela
D
Beriam
Luis O S
LO
Monteiro
Helena Serra Azul
HS
Toyama
Marcos H
MH
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002645:Chickens; D002942:Circular Dichroism; D017839:Crotalus; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008297:Male; D010741:Phospholipases A / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar; D012910:Snake Venoms / Q000201:enzymology*; D014974:Xanthomonas / Q000187:drug effects; D047309:Flavones
D000900:Anti-Bacterial Agents; D005419:Flavonoids; D010741:Phospholipases A; D054467:Phospholipases A2; D012910:Snake Venoms; C008548:morin; D047309:Flavones