[The curative effect of pulmonary surfactant on otitis media with effusion in guinea pigs].
OBJECTIVE
To find a new way of treating otitis media with effusion by studying the curative effect of pulmonary surfactant on secretory otitis media in guinea pigs.


METHODS
Nonviable heat-killed pneumococci (HKP) solution was inoculated into the middle ear cavity in guinea pig via a transeardrum approach, set up models of otitis media with effusion in guinea pigs, after being injected with pulmonary surfactant by transeardrum approach, observed changes of serous effusion in tympanum and response threshold.


RESULTS
Five days after inoculation of HKB, serous effusion was present in the middle ear cavity of guinea pigs with disappearance of light cone. ABR response threshold raised from (14.00 +/- 3.08) dB to (45.00 +/- 5.67) dB. After seven days following injection of pulmonary surfactant by transeardrum approach, serous effusion of tympanum reduced or disappeared. ABR response threshold decreased from (45.00 +/- 5.67) dB to (23.53 +/- 6.32) dB, there was significantly difference between them (P < 0.001). In guinea pigs suffering from otitis media with effusion were not treated by PS, the serous effusion of tympanum did'nt change. Eardrum was more muddy and ABR response threshold raised from (45.00 +/- 5.67) dB before treatment (65.50 +/- 6.85) dB after being treated by PS, respectively, with statistically significant difference between them (P < 0.01).


CONCLUSIONS
Pulmonary surfactant plays an important role in treating otitis media with effusion in guinea pigs.
Huang
Ping
P
Department of Otorhinolaryngology, Yueyang Hospital Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China.
Ma
Zhaoxin
Z
Chen
Xuhui
X
Li
Ming
M
Cao
Yi
Y
Cai
Jianfeng
J
D000818:Animals; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005260:Female; D006168:Guinea Pigs; D008297:Male; D010034:Otitis Media with Effusion / Q000188:drug therapy*; D011663:Pulmonary Surfactants / Q000627:therapeutic use*
D011663:Pulmonary Surfactants