Reference maps of mouse serum acute-phase proteins: changes with LPS-induced inflammation and apolipoprotein A-I and A-II transgenes.
We present reference maps of the mouse serum proteome (run under reducing and non-reducing conditions), from control animals, from mice injected with lipopolysaccharide (LPS) to induce systemic inflammation, and from mice transgenic for human apolipoproteins A-I and A-II. Seventy-seven spots/spot chains from the reducing gels were identified by HPLC MS/MS, representing 28 distinct proteins, including a species-specific protease inhibitor, contrapsin, and high levels of carboxylesterase. The concentrations of acute-phase reactants were monitored for 96 h after LPS challenge. The greatest changes (four-fold 48 h after LPS administration) were observed for haptoglobin and hemopexin. Orosomucoid/alpha(1)-acid glycoprotein and apolipoprotein A-I increased steadily, to 50-60% above baseline at 96 h from stimulation. In mice transgenic for human apolipoprotein A-I the levels of expression of orosomucoid/alpha(1)-acid glycoprotein, alpha(1)-macroglobulin, esterase, kininogen and contrapsin were altered compared to knockout mice lacking apolipoprotein A-I. In contrast, except for the presence of apolipoprotein A-II, no statistically significant difference was observed in mice transgenic for human apolipoprotein A-II.
Wait
Robin
R
Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College London, UK.
Chiesa
Giulia
G
Parolini
Cinzia
C
Miller
Ingrid
I
Begum
Shajna
S
Brambilla
Daniela
D
Galluccio
Lara
L
Ballerio
Rossana
R
Eberini
Ivano
I
Gianazza
Elisabetta
E
D000209:Acute-Phase Proteins / Q000378:metabolism*; D000210:Acute-Phase Reaction; D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics*; D016633:Apolipoprotein A-II / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006242:Haptoglobins / Q000378:metabolism; D006466:Hemopexin / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008822:Mice, Transgenic; D009961:Orosomucoid / Q000378:metabolism; D012016:Reference Values
D000209:Acute-Phase Proteins; D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D006242:Haptoglobins; D008070:Lipopolysaccharides; D009961:Orosomucoid; D006466:Hemopexin