title: Enzyme association with lipidic Langmuir-Blodgett films: interests and applications in nanobioscience.
abstract: This review presents the recent advances in the achievement of organized proteo-lipidic nanostructures based on Langmuir-Blodgett technology and their potential applications in the nanobioscience area. By using the self-assembled properties of amphiphilic biomolecules at the air-water interface, the Langmuir-Blodgett (LB) technique offers the possibility to prepare ultrathin layers suitable for biomolecule immobilization at the molecular level. This review will provide a general overview of the enzyme association with preformed Langmuir-Blodgett films in connection with their potential applications in biosensing device developments, and then introduce the design of a new functionalised biomimetic nanostructure with oriented recognition site. The potential applications of such an organized proteo-lipidic nanostructure for biocatalysis investigations of an immobilised enzyme in a biomimetic situation and for the development of bioelectronic devices are finally discussed.
authors.affiliation: Laboratoire de Génie Enzymatique et Biomoléculaire, EMB2/UMR 5013, CNRS/UCBL, Université Claude Bernard Lyon 1, 43 Bd du 11 novembre 1918, F-69622 Villeurbanne Cedex, France. agnes.egrot@univ-lyon1.fr
authors.forename: Agnès P
authors.initials: AP
authors.lastname: Girard-Egrot
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Godoy
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
mesh_terms: D004798:Enzymes / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008567:Membranes, Artificial; D008954:Models, Biological; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*; D013499:Surface Properties
chemical_list: D004798:Enzymes; D008055:Lipids; D008567:Membranes, Artificial