title: Nucleolar localization of potato leafroll virus capsid proteins.
abstract: Potato leafroll virus (PLRV) encodes two capsid proteins, major protein (CP) and minor protein (P5), an extended version of the CP produced by occasional translational 'readthrough' of the CP gene. Immunogold electron microscopy showed that PLRV CP is located in the cytoplasm and also localized in the nucleus, preferentially targeting the nucleolus. The nucleolar localization of PLRV CP was also confirmed when it was expressed as a fusion with green fluorescent protein (GFP) via an Agrobacterium vector. Mutational analysis identified a particular sequence within PLRV CP involved in nucleolar targeting [the nucleolar localization signal (NoLS)]. Minor protein P5 also contains the same NoLS, and was targeted to the nucleolus when it was expressed as a fusion with GFP from Agrobacterium. However, P5-GFP lost its nucleolar localization in the presence of replicating PLRV.
authors.affiliation: University of Dundee, Dundee DD1 4NH, UK.|Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Sophie
authors.initials: S
authors.lastname: Haupt
authors.affiliation: Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Tanya
authors.initials: T
authors.lastname: Stroganova
authors.affiliation: University of Warwick - HRI, Wellesbourne, Warwick CV35 9EF, UK.
authors.forename: Eugene
authors.initials: E
authors.lastname: Ryabov
authors.affiliation: Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Sang Hyon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Gill
authors.initials: G
authors.lastname: Fraser
authors.affiliation: Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: George
authors.initials: G
authors.lastname: Duncan
authors.affiliation: Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Mike A
authors.initials: MA
authors.lastname: Mayo
authors.affiliation: Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Hugh
authors.initials: H
authors.lastname: Barker
authors.affiliation: Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Taliansky
mesh_terms: D002213:Capsid / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D049452:Green Fluorescent Proteins; D017793:Luteovirus / Q000235:genetics / Q000378:metabolism*; D012231:Rhizobium / Q000821:virology; D011198:Solanum tuberosum / Q000821:virology*
chemical_list: D049452:Green Fluorescent Proteins