title: Trypanosoma cruzi: preliminary investigation of NADH-positive and somatostatin-immunoreactive neurons in the myenteric plexus of the mouse colon during the infection.
abstract: In this paper, the distribution of NADH-positive and somatostatin (SOM) immunoreactive neurons in the myenteric plexus of the colon of mice infected with Trypanosoma cruzi was studied. Ten young, male, BALB/c mice were inoculated with the Y strain of T. cruzi, 60 days previously (chronic phase of the infection). Another 10 mice were uninfected controls. Distal and proximal colonic neurons from five chronically infected mice and their controls were stained using the NADH-diaphorase method. Quantitative results showed a significant decrease of 39% in the number of neurons in the proximal colon of infected mice and 58% in the distal colon (p<0.05). SOM was localized in five animals from each group by light microscopy, using an indirect immunofluorescence technique. It was observed that there were far fewer nerve cells and fibres and less intensely stained neuron bodies and varicose SOM-positive nerve fibres in both, control and chronic infected mice. These findings could be related to the disturbances in intestinal motility observed in patients in the chronic phase of Chagas' disease.
authors.affiliation: Department of Anatomy, Institute of Biomedical Sciences, São Paulo University, São Paulo, Brazil.
authors.forename: L B M
authors.initials: LB
authors.lastname: Maifrino
authors.forename: S O N
authors.initials: SO
authors.lastname: Amaral
authors.forename: I
authors.initials: I
authors.lastname: Watanabe
authors.forename: E A
authors.initials: EA
authors.lastname: Liberti
authors.forename: R R
authors.initials: RR
authors.lastname: De Souza
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000503:physiopathology*; D003106:Colon / Q000294:innervation*; D008058:Dihydrolipoamide Dehydrogenase; D019084:Fluorescent Antibody Technique, Indirect; D006651:Histocytochemistry / Q000379:methods; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009197:Myenteric Plexus / Q000737:chemistry* / Q000166:cytology; D009243:NAD / Q000032:analysis*; D009474:Neurons / Q000737:chemistry*; D013004:Somatostatin / Q000032:analysis*
chemical_list: D009243:NAD; D013004:Somatostatin; D008058:Dihydrolipoamide Dehydrogenase