[Cell differentiation and down regulation of nm23 gene expression in HL-60 cells induced by 1,25 dihydroxyvitamin D3].
OBJECTIVE
To investigate the differentiation induction effect of 1,25 dihydroxyvitamin D3 on leukemia cell line HL-60 and its mechanisms.


METHODS
The changes of proliferation rate and differentiation in HL-60 cells treated with 1,25 dihydroxyvitamin D3 were observed by MTT test, surface marker analysis and NBT reduction reaction, the effects on cell cycle and nm23 gene expression by flow cytometry and RT-PCR.


RESULTS
After exposing to 1,25 dihydroxyvitamin D3, the proliferation was inhibited and the cells were induced to differentiate towards more mature monocytes. During differentiation, HL-60 cells were arrested in G1 phase and the expression of nm23 gene was down regulated.


CONCLUSIONS
1,25 dihydroxyvitamin D3 could induce differentiation of HL-60 cells, this effect might be related to the down regulation of nm23 gene expression.
Zhu
N
N
Deparnent of Hematology , Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310006, China.
Lu
Q
Q
Zhou
Y
Y
D002117:Calcitriol / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics*
D054778:NM23 Nucleoside Diphosphate Kinases; C517938:NME1 protein, human; D002117:Calcitriol