title: [Effect of dendritic cells co-cultured with cytokine induced killer cells on cytotoxicity against drug resistant K562 cells].
abstract: OBJECTIVE
  To study the effect of dendritic cells (DC) co-cultured with cytokine induced killer (CIK) cells on cytotoxicity against K562 and K562 drug-resistant (K562/ADM) cells.
  
  
  METHODS
  Peripheral blood mononuclear cells (MNC) isolated from healthy adult donors were induced to obtain CIK cells and DC respectively and then these two kinds of cells were co-cultured. The cytotoxicity of the co-cultured cells against K562 and K562/ADM cells was measured with MTT assay.
  
  
  RESULTS
  The cytotoxicity CIK cells alone to K562 and K562/ADM cells was (20.0 +/- 1.2)% - (61.1 +/- 2.2)% and (17.5 +/- 2.1)% - (45.2 +/- 3.3)% respectively at low effector to target ratios (2.5 - 20.0). This effect was significantly enhanced by co-culturing with DCs being (25.2 +/- 2.3)% - (70.9 +/- 4.1)% and (22.4 +/- 2.7)% - (62.3 +/- 5.0)%.
  
  
  CONCLUSIONS
  CIK cells showed high cytotoxicity against K562 and K562/ADM cells and the activity could be enhanced by co-culturing with DC.
authors.affiliation: The Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, China.
authors.forename: Bao-an
authors.initials: BA
authors.lastname: Chen
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.forename: Zai-yang
authors.initials: ZY
authors.lastname: Sun
authors.forename: Cui-ping
authors.initials: CP
authors.lastname: Li
authors.forename: Chong
authors.initials: C
authors.lastname: Gao
authors.forename: Yun-yu
authors.initials: YY
authors.lastname: Sun
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D055612:Cytokine-Induced Killer Cells / Q000166:cytology / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D020014:K562 Cells