Cloning, tissue expression and metal inducibility of an ubiquitous metallothionein from Panulirus argus.
Invertebrate metallothioneins (MT) have mainly been reported in digestive tissues, but data about the existence of a ubiquitous isoform expressed also in nervous tissue, are not available. Here we report the identification of a new metallothionein gene (MTPA) from the lobster Panulirus argus, putatively encoding a 59 residue polypeptide including 19 Cys. Tissue specific analysis indicated that MTPA is ubiquitously expressed in the hepatopancreas, intestine, nervous tissue and muscle, with the highest levels in the hepatopancreas and the lowest in muscle and nervous tissue. In addition, our data showed that MTPA is differentially regulated by metals: tissue explants exposed to Cd exhibited increased MTPA mRNA levels in all cases, except in muscle, with the highest effects in the nervous tissue, while Zn was effective only in the hepatopancreas. Interestingly, Cu showed no effects in any of the analyzed tissues. Taken together, these results suggest that MTPA in the hepatopancreas likely plays an important role in Cd detoxification and Zn homeostasis. The potent Cd-inducibility of MTPA in the nervous tissue might suggest a key function of this protein in protecting this highly sensitive tissue from cadmium-induced neurotoxicity.
Moltó
Eduardo
E
Area de Bioquímica, Facultad de Químicas, Centro Regional de Investigaciones Biomédicas (CRIB), Universidad de Castilla-La Mancha, Camilo José Cela, 10, 13071 Ciudad Real, Spain.
Bonzón-Kulichenko
Elena
E
del Arco
Araceli
A
López-Alañón
Dulce M
DM
Carrillo
Olimpia
O
Gallardo
Nilda
N
Andrés
Antonio
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002104:Cadmium / Q000633:toxicity; D003001:Cloning, Molecular; D003300:Copper / Q000633:toxicity; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D008668:Metallothionein / Q000235:genetics*; D019216:Metals, Heavy / Q000633:toxicity*; D008969:Molecular Sequence Data; D033521:Palinuridae / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015032:Zinc / Q000633:toxicity
D018076:DNA, Complementary; D019216:Metals, Heavy; D012333:RNA, Messenger; D002104:Cadmium; D003300:Copper; D008668:Metallothionein; D015032:Zinc