title: Potassium channel Kir4.1 macromolecular complex in retinal glial cells.
abstract: A major role for Müller cells in the retina is to buffer changes in the extracellular K+ concentration ([K+]o) resulting from light-evoked neuronal activity. The primary K+ conductance in Müller cells is the inwardly rectifying K+ channel Kir4.1. Since this channel is constitutively active, K+ can enter or exit Müller cells depending on the state of the [K+]o. This process of [K+]o buffering by Müller cells ("K+ siphoning") is enhanced by the precise accumulation of these K+ channels at discrete subdomains of Müller cell membranes. Specifically, Kir4.1 is localized to the perivascular processes of Müller cells in animals with vascular retinas and to the endfeet of Müller cells in all species examined. The water channel aquaporin-4 (AQP4) also appears to be important for [K+]o buffering and is expressed in Müller cells in a very similar subcellular distribution pattern to that of Kir4.1. To gain a better understanding of how Müller cells selectively target K+ and water channels to discrete membrane subdomains, we addressed the question of whether Kir4.1 and AQP4 associate with the dystrophin-glycoprotein complex (DGC) in the mammalian retina. Immunoprecipitation (IP) experiments were utilized to show that Kir4.1 and AQP4 are associated with DGC proteins in rat retina. Furthermore, AQP4 was also shown to co-precipitate with Kir4.1, suggesting that both channels are tethered together by the DGC in Müller cells. This work further defines a subcellular localization mechanism in Müller cells that facilitates [K+]o buffering in the retina.
authors.affiliation: Department of Neuroscience, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Connors
authors.forename: Paulo
authors.initials: P
authors.lastname: Kofuji
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000378:metabolism; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000378:metabolism; D003432:Cross-Linking Reagents; D016189:Dystrophin / Q000502:physiology; D049029:Dystrophin-Associated Proteins / Q000378:metabolism; D027341:Excitatory Amino Acid Transporter 1 / Q000378:metabolism; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D009124:Muscle Proteins / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism*; D011817:Rabbits; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D000098854:Kcnj10 Channel
chemical_list: D051401:Aquaporin 4; D002135:Calcium-Binding Proteins; D003432:Cross-Linking Reagents; D016189:Dystrophin; D049029:Dystrophin-Associated Proteins; D027341:Excitatory Amino Acid Transporter 1; D000098854:Kcnj10 Channel; D008565:Membrane Proteins; D009124:Muscle Proteins; D024661:Potassium Channels, Inwardly Rectifying; C493393:Slc1a3 protein, mouse; C098885:dystrobrevin; C091825:syntrophin alpha1