title: Protein profiles of bovine placenta derived from somatic cell nuclear transfer.
abstract: Practical application of animal cloning by somatic cell nuclear transfer (SCNT) has been hampered by an extremely low success rate. To address whether placental dysfunction in SCNT causes fetal loss during pregnancy, we have used a global proteomics approach using 2-DE and MS to analyze the differential protein patterns of three placentae from the afterbirth of cases of postnatal death, derived from SCNT of Korean Native cattle, and three normal placentae obtained from the afterbirth of fetuses derived from artificial insemination. Proteins within a pI range of 4.0-7.0 and 6.0-9.0 were analyzed separately by 2-DE in triplicate. A total of approximately 2000 spots were detected in placental 2-DE gels stained with CBB. In the comparison of normal and SCNT samples, 60 spots were identified as differentially expressed proteins, of which 33 spots were up-regulated proteins in SCNT placentae, while 27 spots were down-regulated proteins. Most of the proteins identified in this analysis appeared to be related with protein repair or protection, cytoskeleton, signal transduction, immune system, metabolism, extracellular matrix and remodeling, transcription regulation, cell structure or differentiation and ion transport. One of up-regulated proteins in SCNT was TIMP-2 protein known to be related to extracellular matrix and remodeling during pregnancy. Western blot analysis showed an increased level of TIMP-2 in SCNT placenta compared to normal. Our results revealed composite profiles of key proteins involved in abnormal placenta derived from SCNT, and suggested expression abnormality of these genes in SCNT placenta, resulting in fetal losses following SCNT.
authors.affiliation: Research Center for Transgenic Cloned Pigs, Chungnam National University, Yuseong-Gu, Daejeon, Korea.
authors.forename: Hong Rye
authors.initials: HR
authors.lastname: Kim
authors.forename: Jae Ku
authors.initials: JK
authors.lastname: Kang
authors.forename: Jong Taek
authors.initials: JT
authors.lastname: Yoon
authors.forename: Hwan Hoo
authors.initials: HH
authors.lastname: Seong
authors.forename: Jin Kwan
authors.initials: JK
authors.lastname: Jung
authors.forename: Hong Mie
authors.initials: HM
authors.lastname: Lee
authors.forename: Chang
authors.initials: C
authors.lastname: Sik Park
authors.forename: Dong Il
authors.initials: DI
authors.lastname: Jin
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D007315:Insemination, Artificial; D053652:Nuclear Transfer Techniques; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism
chemical_list: D011257:Pregnancy Proteins; D019716:Tissue Inhibitor of Metalloproteinase-2