title: Resonance light scattering technique for the determination of proteins with Congo red and Triton X-100.
abstract: Resonance light scattering (RLS) of Congo red (CR) was greatly enhanced by BSA (HSA) in the presence of Triton X-100 (TX-100). In sodium citrate-HCl buffer (pH 2.7-3.0), the enhanced intensity of resonance light scattering at 360 nm was in proportion to the concentration of proteins [corrected] The linear relationship was obtained between the resonance light scattering intensity and proteins in the range 5.0 x 10(-8)-8.0 x 10(-6) g/mL and 1.0 x 10(-9)-6.0 x 10(-6) g/mL for BSA and HSA, respectively. Their detection limits were 1.4 x 10(-8) g/mL and 2.8 x 10(-10) g/mL (S:N = 3), respectively. Synthetic and actual samples were analysed satisfactorily.
authors.affiliation: Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Shuna
authors.initials: S
authors.lastname: Sun
authors.forename: Changying
authors.initials: C
authors.lastname: Guo
authors.forename: Jinghe
authors.initials: J
authors.lastname: Yang
authors.forename: Changxia
authors.initials: C
authors.lastname: Sun
authors.forename: Chengrong
authors.initials: C
authors.lastname: Zhou
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
mesh_terms: D002021:Buffers; D003224:Congo Red / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008027:Light; D046529:Microscopy, Electron, Transmission; D017830:Octoxynol / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity
chemical_list: D002021:Buffers; D011506:Proteins; D003224:Congo Red; D017830:Octoxynol