title: Epidermal hyperpigmentation in non-syndromic solitary cafe-au-lait macules may be associated with increased secretion of endothelin-1 by lesional keratinocytes.
abstract: To clarify the mechanism of accentuated melanisation in non-syndromic solitary cafe-au-lait macules we used an enzyme-linked immunosorbent assay (ELISA) to measure the concentration of melanogenic cytokines secreted by cultured keratinocytes and fibroblasts derived from the skins of the macules and compared them with those derived from normal people. Endothelin-1 (ET-1) was significantly increased in cultured keratinocytes in the macules compared with the normals. In contrast, the secretion of other cytokines secreted by keratinocytes or fibroblasts did not differ between the groups. It may be that the increased secretion of ET-1 by epidermal keratinocytes has a role in the accentuated epidermal melanisation seen in non-syndromic macules.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, University of Tokyo, Japan. okazaki-m@umin.ac.jp
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Okazaki
authors.forename: Kotaro
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Gentaro
authors.initials: G
authors.lastname: Uchida
authors.forename: Yasutoshi
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Yukie
authors.initials: Y
authors.lastname: Kitano
authors.forename: Kiyonori
authors.initials: K
authors.lastname: Harii
mesh_terms: D000293:Adolescent; D000328:Adult; D019080:Cafe-au-Lait Spots / Q000473:pathology / Q000503:physiopathology*; D002478:Cells, Cultured; D002648:Child; D019332:Endothelin-1 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004817:Epidermis; D005260:Female; D006801:Humans; D017495:Hyperpigmentation / Q000503:physiopathology*; D015603:Keratinocytes / Q000378:metabolism*; D008297:Male
chemical_list: D019332:Endothelin-1