Large-scale isolation and purification of geniposide from the fruit of Gardenia jasminoides Ellis by high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of geniposide from Gardenia jasminoides Ellis. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of ethyl acetate-n-butanol-water (2:1:3, v/v/v). According to the above solvent system, preparative HSCCC was successfully performed with the optimal solvent system composed of ethyl acetate-n-butanol-water (2:1.5:3, v/v/v) yielding 389 mg of geniposide at over 98% purity from 1g of the partially purified extract with 38.9% recovery in a one-step separation.
Zhou
Tingting
T
Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai, China.
Fan
Guorong
G
Hong
Zhanying
Z
Chai
Yifeng
Y
Wu
Yutian
Y
D003377:Countercurrent Distribution / Q000379:methods*; D032062:Gardenia / Q000737:chemistry*; D039823:Iridoids / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D011714:Pyrans / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization
D039823:Iridoids; D011714:Pyrans; C007835:geniposide