Triterpenoid from Styrax japonica SIEB. et ZUCC, and its effects on the expression of matrix metalloproteinases-1 and type 1 procollagen caused by ultraviolet irradiated cultured primary human skin fibroblasts.
Long term and repeated exposure of ultraviolet light on the skin often induces chronic skin diseases such as skin cancer as well as photoaging, and the mechanisms of these skin damages are closely associated with up-regulation of matrix metalloproteinase's (MMPs) activities. The methylene chloride soluble fraction of methanol extract from the stems of Styrax japonica. (Styracaceae) showed significant MMP-1 inhibition in primary human skin fibroblasts cause by ultraviolet irradiation. Four triterpenoids were isolated by column chromatography. Among them, Erythrodiol-3-acetate reduced of MMP-1 and induced of type 1 procollagen at the protein levels in a dose-dependent manner.
Moon
Hyung-In
HI
Department of Dermatology, Seoul National University College of Medicine; Seoul, 110-744, Korea. himun@snu.ac.kr
Kim
Mi-Ran
MR
Woo
Eun-Ran
ER
Chung
Jin Ho
JH
D016475:3T3 Cells; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects; D032362:Styrax / Q000737:chemistry*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D014466:Ultraviolet Rays
D024042:Collagen Type I; D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors; D014315:Triterpenes; D008156:Luciferases