title: Effects of ascorbic acid on the vasoactive intestinal peptide synthesis in the ileum submucous plexus of normal rats.
abstract: BACKGROUND
  The aging process is a deteriorating process that attacks the gastrointestinal tract, causing changes in the number and size of neurons from the enteric nervous system. The activity of free radicals on enteric neurons is helped by the significant reduction of antioxidants.
  
  
  OBJECTIVE
  Evaluate the effect of the ascorbic acid supplementation on the neurons that produce the vasoactive intestinal peptide (VIP) in the submucous plexus of the ileum of normal rats for a period of 120 days.
  
  
  METHODS
  Fifteen rats were divided in three groups: untreated control with 90 days, untreated control with 210 days and ascorbic acid-treated rats with 210 days. Ascorbic acid was given for 16 weeks from the 90th day of age by adding it to drinking water (1 g/L prepared fresh each day). The ileums were processed according to the immunohistochemistry technique for whole-mount preparation in order to detect the presence of VIP immunoreactive in the cellular bodies and nervous fibers in the neurons of the submucous plexus. We have verified their immunoreactivity and measured the cellular profile of 80 cellular bodies of VIP-ergic neurons from each studied group.
  
  
  RESULTS
  The ascorbic acid supplementation did not alter physiological parameters such as water intake and food consumption of the three studied groups. We observed a significant increase of the cellular profile of VIP-ergic neurons in untreated control with 210 days when compared to untreated control with 90 days. The cellular profile of VIP-ergic neurons in ascorbic acid-treated rats with 210 days was bigger than those observed in others groups.
  
  
  CONCLUSIONS
  The ascorbic acid had a neurotrophic effect on VIP-ergic neurons on the ileum after period 120 days of supplementation.
authors.affiliation: Department of Morphophysiological Sciences, State University of Maringá, Maringá, PR, Brazil. jnzanoni@uem.br
authors.forename: Jacqueline Nelisis
authors.initials: JN
authors.lastname: Zanoni
authors.forename: Priscila de
authors.initials: Pd
authors.lastname: Freitas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D019587:Dietary Supplements; D007082:Ileum / Q000294:innervation*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013368:Submucous Plexus / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000096:biosynthesis* / Q000187:drug effects
chemical_list: D000975:Antioxidants; D014660:Vasoactive Intestinal Peptide; D001205:Ascorbic Acid