Inhibitory effects of Morus alba on compound 48/80-induced anaphylactic reactions and anti-chicken gamma globulin IgE- mediated mast cell activation.
We investigated the effects of hot-water extract from the root bark of Morus alba (HEMA) on anaphylactic reactions. Using in vitro and in vivo experiments, we examined whether HEMA could inhibit compound 48/80-induced systemic anaphylactic shock and anti-chicken gamma globulin (CGG) IgE-mediated rat peritoneal mast cell activation. HEMA significantly inhibited systemic anaphylaxis induced by the compound 48/80 in mice. HEMA also significantly inhibited the passive cutaneous anaphylaxis activated by anti-CGG IgE. HEMA had no cytotoxicity on rat peritoneal mast cells (RPMC). Moreover, HEMA dose-dependently inhibited mast cell degranulation, histamine release and calcium uptake into RPMC induced by the compound 48/80 or anti-CGG IgE. When HEMA was added, the level of intracellular cAMP in RPMC showed a transient and significant increase (5-fold) compared with that of control cells. HEMA also inhibited significantly the compound 48/80-induced cAMP reduction in RPMC. These results suggested that HEMA inhibits the compound 48/80- or anti-CGG IgE-induced mast cell activation and its inhibitory effects on mast cell activations were favorably comparable to disodium cromoglycate. And HEMA is a candidate for effective therapeutic tools of allergic diseases.
Chai
Ok Hee
OH
Department of Anatomy, Chonbuk National University Medical School; Republic of Korea.
Lee
Moo Sam
MS
Han
Eui-Hyeog
EH
Kim
Hyoung Tae
HT
Song
Chang Ho
CH
D000707:Anaphylaxis / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D031625:Morus / Q000737:chemistry*; D051381:Rats; D005719:gamma-Globulins / Q000276:immunology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D005719:gamma-Globulins; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D000242:Cyclic AMP