[Identification of trehalose-phosphate phosphatase associated with drug-resistance from culture supernatants of isoniazid-resistant Mycobacterium tuberculosis].
OBJECTIVE
To isolate and identify a new protein involved in drug-resistance from culture supernatants of isoniazid (INH)-resistant Mycobacterium tuberculosis.


METHODS
Bovine albumin of Middlebrook 7H10 culture supernatants of INH-resistant Mycobacterium tuberculosis was removed using diethylamine ethylcellulose affinitive gel chromatography. A specific protein from culture supernatants of three INH-resistant Mycobacterium tuberculosis isolates was separated reproducibly by high performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide electrophoresis, and identified by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-MS).


RESULTS
The protein was trehalose-phosphate phosphatase involved in the biosynthesis of trehalose of cell wall, molecular a eight 46000, isoelectric point 5.3. It could be detected repeatedly from supernatants of three INH resistant Mycobacterium tuberculosis isolates.


CONCLUSIONS
Trehalose-phosphate phosphatase represents an excellent potential target site for chemotherapy against Mycobacterium tuberculosis and a new marker in the detection of drug resistance of Mycobacterium tuberculosis.
Yue
Jun
J
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Llife Science, Fudan University, Shanghai, China.
Liu
Li-rong
LR
Xie
Jian-ping
JP
Lei
Jian-qiang
JQ
Liang
Li
L
Wang
Hong-hai
HH
D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology*; D002417:Cattle; D004269:DNA, Bacterial; D007538:Isoniazid / Q000494:pharmacology*; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D010744:Phosphoric Monoester Hydrolases / Q000302:isolation & purification*; D018088:Tuberculosis, Multidrug-Resistant
D000995:Antitubercular Agents; D004269:DNA, Bacterial; C023178:trehalose-phosphatase; D010744:Phosphoric Monoester Hydrolases; D007538:Isoniazid