title: Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens.
abstract: We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a Light Cycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use will aid in the rapid diagnosis of invasive aspergillosis.
authors.affiliation: NHMRC Clinical Center of Research Excellence in Infectious Diseases, Hematology, and Health Ethics, University of Sydney Western Clinical School, New South Wales 2145, Australia. catrionah@icpmr.wsahs.nsw.gov.au
authors.forename: Catriona
authors.initials: C
authors.lastname: Halliday
authors.forename: Qi Xuan
authors.initials: QX
authors.lastname: Wu
authors.forename: Gregory
authors.initials: G
authors.lastname: James
authors.forename: Tania
authors.initials: T
authors.lastname: Sorrell
mesh_terms: D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology*; D001230:Aspergillus / Q000235:genetics / Q000302:isolation & purification*; D001769:Blood / Q000382:microbiology; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D015342:DNA Probes; D004271:DNA, Fungal