title: Pb enamel biomarker: deposition of pre- and postnatal Pb isotope injection in reconstructed time points along rat enamel transect.
abstract: Exposure to lead (Pb) as well as other heavy metals in the environment is still a matter of public health concern. The development of the enamel biomarker for heavy metal exposure assessment is designed to improve studies of dose-effect relationships to developmental anomalies, particularly embryonic dysfunctions, and to provide a time-specific recount of past exposures. The work presented in this paper demonstrates maternal transfer across the placental barrier of the enriched isotope (206)Pb tracer to the enamel of the rat pup. Likewise, injections of (204)Pb-enriched tracer in the neonate rat resulted in deposition of the tracer in the enamel histology as measured by secondary ion microprobe spectrometry. Through enamel, we were able to observe biological removal and assimilation of prenatal and postnatal tracers, respectively. This research demonstrates that enamel can be used as a biomarker of exposure to Pb and may illustrate the toxicokinetics of incorporating Pb into fetal and neonatal steady-state system processes. The biomarker technique, when completely developed, may be applied to cross-sectional and longitudinal epidemiological research.
authors.affiliation: Department of Environmental Health, Science, and Policy, School of Social Ecology, University of California, Irvine, CA 92697-7070, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Rinderknecht
authors.forename: M T
authors.initials: MT
authors.lastname: Kleinman
authors.forename: J E
authors.initials: JE
authors.lastname: Ericson
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers; D003743:Dental Enamel / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D007180:Incisor; D007554:Isotopes; D007854:Lead / Q000493:pharmacokinetics*; D008297:Male; D018811:Maternal Exposure; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D007554:Isotopes; D007854:Lead