[Expression of Oct2 and its significance in lymphoma diagnosis].
OBJECTIVE
To investigate the specificity and sensitivity of Oct2 protein expression in lymphoma cells and its significance in diagnosis and classification of lymphoma.


METHODS
Formalin-fixed and paraffin-embedded materials from 129 cases of lymphoma and 10 cases of reactive lymphoid hyperplasia (RLH) were studied by EnVision immunohistochemistry for Oct2 protein.


RESULTS
Oct2 was mainly expressed in germinal center cells of RLH. It was diffusely expressed in B-cell lymphoma cells. 97.7% cases (85/87) of B-cell lymphoma and 3.8% cases (1/26) of T-cell lymphoma were positive for Oct2 protein. In comparison, the expression rates for CD20 and CD79alpha in B-cell lymphomas were 90.8% (79/87) and 84.7% (61/72) respectively. The difference in expression rates between Oct2 protein and CD20 was not statistically significant (P > 0.05) There was, however, significant difference in expression rates between Oct2 protein and CD79alpha (P < 0.05). The expression rates of Oct2 protein in nodular lymphocyte-predominant Hodgkin lymphoma and classic Hodgkin lymphoma were 3/3 and 46.2% (6/13) respectively. The difference in expression rates of Oct2 protein in these two groups showed no statistical significance (P > 0.05).


CONCLUSIONS
As a relatively sensitive and specific marker for B cells, Oct2 can serve as a useful antibody for the diagnosis and differential diagnosis of lymphoma.
Zhao
Xiang-rong
XR
Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Ma
Da-lie
DL
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018951:Antigens, CD20 / Q000378:metabolism; D051925:CD79 Antigens / Q000378:metabolism; D002648:Child; D003937:Diagnosis, Differential; D005260:Female; D018858:Germinal Center / Q000378:metabolism; D006689:Hodgkin Disease / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D008223:Lymphoma / Q000145:classification / Q000175:diagnosis / Q000378:metabolism*; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000378:metabolism*; D016399:Lymphoma, T-Cell / Q000175:diagnosis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D050813:Octamer Transcription Factor-2 / Q000378:metabolism*; D019310:Pseudolymphoma / Q000175:diagnosis / Q000378:metabolism
D018951:Antigens, CD20; D051925:CD79 Antigens; C497881:CD79A protein, human; D050813:Octamer Transcription Factor-2