An immunogold evaluation of cortical actin reorganization in pubertal Sertoli cells in vitro after PMA treatment.
The aim of the present study was to investigate stress fibers and cortical actin reorganization in pubertal Sertoli cells in vitro after PMA treatment. Actin was studied by means of immunogold labeling, using the 'Progressive Lowering of Temperature' technique (PLT). Actin rearrangement was evaluated by a quantitative analysis of the gold label distribution. Eight hours after addition of 10(-7) M PMA, rearrangement of cortical actin was minimal, but stress fiber perturbation was significant as shown by immunogold labeling distribution measurements. PMA-mediated F-actin reorganization and redistribution in non-neoplastic cells is discussed, since these phenomena have been closely linked with cell transformation.
Panagopoulou
Evridiki
E
University of Athens, Medical School, Department of Histology and Embryology, Mikras Asias 75, Goudi, Greece.
Kouloukoussa
Mirsini
M
Kittas
Christos
C
Marinos
Evangelos
E
D008841:Actin Cytoskeleton / Q000187:drug effects; D000199:Actins / Q000737:chemistry*; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000166:cytology* / Q000378:metabolism; D013696:Temperature; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors
D000199:Actins; D002273:Carcinogens; D013755:Tetradecanoylphorbol Acetate