Use of specific rRNA oligonucleotide probes for microscopic detection of Mycobacterium tuberculosis in culture and tissue specimens.
Mycobacterium tuberculosis infections are a major global health problem. Fast and accurate detection of M. tuberculosis is clearly needed at both the clinical level and the research level. We report a rapid and reliable in situ hybridization technique using rRNA oligonucleotide probes for the identification of M. tuberculosis in tissues and cultures.
St Amand
Allison L
AL
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA.
Frank
Daniel N
DN
De Groote
Mary Ann
MA
Basaraba
Randall J
RJ
Orme
Ian M
IM
Pace
Norman R
NR
D000818:Animals; D002417:Cattle; D003470:Culture Media; D006168:Guinea Pigs; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008168:Lung / Q000382:microbiology; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D015345:Oligonucleotide Probes / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000382:microbiology
D003470:Culture Media; D015345:Oligonucleotide Probes; D012335:RNA, Ribosomal