Separation of monosaccharides by hydrophilic interaction chromatography with evaporative light scattering detection.
Hydrophilic interaction liquid chromatography (HILIC) was used to separate monosaccharides that are common in N-linked oligosaccharides in glycoproteins and other compounds. A TSKgel Amide-80 column was eluted with 82% acetonitrile, in 5 mM ammonium formate (pH 5.5). Column temperature was 60 degrees C and evaporative light scattering was used for detection (ELSD). With this method, L-fucose, D-galactose, D-mannose, N-acetyl-D-glucosamine, N-acetylneuraminic acid, and D-glucuronic acid were separated, with detection limits of 0.3-0.5 microg for each monosaccharide, and intermediate precisions were 3-6% RSD (n=6).
Karlsson
Göran
G
Octapharma AB, SE-11275 Stockholm, Sweden. goran.karlsson@octapharma.se
Winge
Stefan
S
Sandberg
Helena
H
D002853:Chromatography, Liquid / Q000379:methods*; D008027:Light; D009005:Monosaccharides / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity
D009005:Monosaccharides