title: Sonochemically fabricated microelectrode arrays for biosensors. Part III. AC impedimetric study of aerobic and anaerobic response of alcohol oxidase within polyaniline.
abstract: A sonochemically fabricated alcohol oxidase enzyme micro-electrode array is reported. Sensors of this type were fabricated by first depositing an insulating polydiaminobenzene film on supporting gold electrodes. Sonication and subsequent ablation exposed discrete areas of the underlying conducting electrode, which collectively act as a microelectrode array. Electropolymerisation of aniline has been used to generate in situ polyaniline containing entrapped alcohol oxidase. The physical and electrochemical properties of these films were studied and reported within this paper. The final composites were shown to behave with microelectrode performance characteristics for the detection of aqueous ethanol concentrations.
authors.affiliation: Institute of Biosciences and Technology, Cranfield University, Silsoe, Beds MK45 4ST, UK.
authors.forename: Suzy
authors.initials: S
authors.lastname: Myler
authors.forename: Stuart D
authors.initials: SD
authors.lastname: Collyer
authors.forename: Frank
authors.initials: F
authors.lastname: Davis
authors.forename: Davinia D
authors.initials: DD
authors.lastname: Gornall
authors.forename: Seamus P J
authors.initials: SP
authors.lastname: Higson
mesh_terms: D000332:Aerobiosis; D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D000693:Anaerobiosis; D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017097:Electric Impedance; D004574:Electromagnetic Fields; D004867:Equipment Design; D019544:Equipment Failure Analysis; D000431:Ethanol / Q000032:analysis* / Q000737:chemistry*; D008839:Microelectrodes; D013010:Sonication; D014867:Water / Q000737:chemistry
chemical_list: D000814:Aniline Compounds; D020099:Coated Materials, Biocompatible; C416807:polyaniline; D014867:Water; D000431:Ethanol; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase