title: Histone H3 lysine 4 mono-methylation does not require ubiquitination of histone H2B.
abstract: The yeast Set1-complex catalyzes histone H3 lysine 4 (H3K4) methylation. Using N-terminal Edman sequencing, we determined that 50% of H3K4 is methylated and consists of roughly equal amounts of mono, di and tri-methylated H3K4. We further show that loss of either Paf1 of the Paf1 elongation complex, or ubiquitination of histone H2B, has only a modest effect on bulk histone mono-methylation at H3K4. Despite the fact that Set1 recruitment decreases in paf1delta cells, loss of Paf1 results in an increase of H3K4 mono-methylation at the 5' coding region of active genes, suggesting a Paf1-independent targeting of Set1. In contrast to Paf1 inactivation, deleting RTF1 affects H3K4 mono-methylation at the 3' coding region of active genes and results in a decrease of global H3K4 mono-methylation. Our results indicate that the requirements for mono-methylation are distinct from those for H3K4 di and tri-methylation, and point to differences among members of the Paf1 complex in the regulation of H3K4 methylation.
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires et Institut de Biologie Structurale et Microbiologie, CNRS, 31 chemin Joseph Aiguier, 13402, Marseille, Cedex 20, France.
authors.forename: Pierre-Marie
authors.initials: PM
authors.lastname: Dehé
authors.forename: Mercè
authors.initials: M
authors.lastname: Pamblanco
authors.forename: Pierre
authors.initials: P
authors.lastname: Luciano
authors.forename: Régine
authors.initials: R
authors.lastname: Lebrun
authors.forename: Danièle
authors.initials: D
authors.lastname: Moinier
authors.forename: Ramon
authors.initials: R
authors.lastname: Sendra
authors.forename: Alain
authors.initials: A
authors.lastname: Verreault
authors.forename: Vicente
authors.initials: V
authors.lastname: Tordera
authors.forename: Vincent
authors.initials: V
authors.lastname: Géli
mesh_terms: D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008745:Methylation; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D006657:Histones; D025801:Ubiquitin; D008239:Lysine