Expression patterns of the DAZ-associated protein DAZAP1 in rat and human ovaries.
OBJECTIVE
To evaluate the expression of DAZAP1 (deleted in azoospermia-associated protein 1) in rat and human ovaries.


METHODS
Experimental study.


METHODS
University hospital.


METHODS
Twelve corpus luteum (CL) specimens were collected during operation, either by laparoscopic surgery for CL rupture or by laparotomy for benign gynecologic conditions.


METHODS
Surgical excision of 12 human CL.


METHODS
Proteins analyzed by immunohistochemical staining, Western blotting, and co-immunoprecipitation experiments.


RESULTS
DAZAP1 is expressed in rat and human luteal cells. Expression of DAZAP1 decreases with advancing stages of CL. Co-immunoprecipitation experiments show in vivo interaction of DAZ-like (DAZL) protein with DAZAP1 in the ovarian tissues.


CONCLUSIONS
The expression patterns of DAZAP1 and DAZL are identical within rat and human ovaries. In mammalian species, DAZAP1 may be involved in diverse reproductive functions, ranging from cell cycle regulation and maturation of oocytes to differentiation of luteal cells.
Pan
Hsien-An
HA
Department of Obstetrics and Gynecology, Institute of Clinical Medicine, Tainan, Taiwan.
Lin
Yue-Shan
YS
Lee
Ko-Hung
KH
Huang
Jin-Ru
JR
Lin
Ying-Hui
YH
Kuo
Pao-Lin
PL
D000818:Animals; D000075364:Deleted in Azoospermia 1 Protein; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D010053:Ovary / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid
C095668:DAZ1 protein, human; C101358:DAZL protein, human; D000075364:Deleted in Azoospermia 1 Protein; D016601:RNA-Binding Proteins