A 'Chinese Spring' wheat (Triticum aestivum L.) bacterial artificial chromosome library and its use in the isolation of SSR markers for targeted genome regions.
A bacterial artificial chromosome (BAC) library was constructed from the bread wheat (Triticum aestivum L.) genotype 'Chinese Spring' ('CS'). The library consists of 395,136 clones with an estimated average insert size of 157 kb. This library provides an estimated 3.4-fold genome coverage for this hexaploid species. The genome coverage was confirmed by RFLP analysis of single-copy RFLP clones. The CS BAC library was used to develop simple sequence repeat (SSR) markers for targeted genome regions using five sequence-tagged-site (STS) markers designed from the chromosome arm of 3BS. The SSR markers for the targeted genome region were successfully obtained. However, similar numbers of new SSR markers were also generated for the other two homologous group 3 chromosomes. This data suggests that BAC clones belonging to all three chromosomes of homologous group 3 were isolated using the five STS primers. The potential impacts of these results on marker isolation in wheat and on library screening in general are discussed.
Shen
B
B
CSIRO Plant Industry, 306 Carmody Road, St Lucia, Queensland, 4067, Australia.
Wang
D M
DM
McIntyre
C L
CL
Liu
C J
CJ
D022202:Chromosomes, Artificial, Bacterial; D017931:DNA Primers; D015723:Gene Library; D018745:Genome, Plant / Q000235:genetics*; D023281:Genomics / Q000379:methods; D018598:Minisatellite Repeats / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers