title: Detection of CD5 in B-cell chronic lymphoproliferative diseases by flow cytometry: a strong expression in B-cell chronic lymphocytic leukemia.
abstract: OBJECTIVE
  CD5 is a T cell marker, aberrantly express in B cell chronic lymphocytic leukemia (B-CLL) and mantle cell lymphoma (MCL). Other chronic B cell malignancies including hairy cell leukemia (HCL) and B cell prolymphocytic leukemia (B-PLL) are CD5 negative or express this antigen in a weak way. In this study, CD5 expression was investigated in leukemic cells from 42 patients with chronic B cell lymphoproliferative disease.
  
  
  METHODS
  We studied the CD5 expression in leukemic cells from 42 patients with chronic B-cell malignancies by flow cytometry. Demographic features such as age, sex and clinical date were also analyzed.
  
  
  RESULTS
  There were 22 males and 20 females. The immunophenotyping showed that 35 cases were B-CLL, 3 B-PLL and HCL and one patient was MCL. CD5 expression was present in all B-CLL and MCL. Low expression of CD5 was observed in one patient with B-PLL and negative in all cases of HCL.
  
  
  CONCLUSIONS
  Our date demonstrated that CD5 expression can help distinguish among B-CLL from HCL and B-PLL, but is similar expressed in MCL.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas da Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil. gbcjunior@hotmail.com
authors.forename: Geraldo Barroso
authors.initials: GB
authors.lastname: Cavalcanti Júnior
authors.forename: Valeria Soraya de Farias
authors.initials: VS
authors.lastname: Sales
authors.forename: Dany Geraldo Kramer
authors.initials: DG
authors.lastname: Cavalcanti e Silva
authors.forename: Maria Cleide de Araújo
authors.initials: MC
authors.lastname: Lopes
authors.forename: Aldair de Souza
authors.initials: Ade S
authors.lastname: Paiva
authors.forename: Henrique Eduardo Macedo
authors.initials: HE
authors.lastname: da Fonseca
authors.forename: Francisco Fernandes
authors.initials: FF
authors.lastname: do Nascimento Júniors
authors.forename: Maria Zélia
authors.initials: MZ
authors.lastname: Fernandes
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood; D018956:CD5 Antigens / Q000097:blood*; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping; D007943:Leukemia, Hairy Cell / Q000097:blood / Q000175:diagnosis; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000175:diagnosis*; D018655:Lymphocyte Count; D016393:Lymphoma, B-Cell / Q000097:blood / Q000175:diagnosis*; D020522:Lymphoma, Mantle-Cell / Q000097:blood / Q000175:diagnosis; D008232:Lymphoproliferative Disorders / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged
chemical_list: D014408:Biomarkers, Tumor; D018956:CD5 Antigens