MDR-like ABC transporter AtPGP4 is involved in auxin-mediated lateral root and root hair development.
Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss-of-function plants reveal enhanced lateral root initiation and root hair lengths both known to be under the control of auxin. Further, atpgp4 plants show altered sensitivities toward auxin and the auxin transport inhibitor, NPA. Finally, mutant roots reveal elevated free auxin levels and reduced auxin transport capacities. These results together with yeast growth assays suggest a direct involvement of AtPGP4 in auxin transport processes controlling lateral root and root hair development.
Santelia
Diana
D
Zurich-Basel Plant Science Center, Institute of Plant Biology, Molecular Plant Physiology, University of Zürich, Switzerland.
Vincenzetti
Vincent
V
Azzarello
Elisa
E
Bovet
Lucien
L
Fukao
Yoichiro
Y
Düchtig
Petra
P
Mancuso
Stefano
S
Martinoia
Enrico
E
Geisler
Markus
M
D018528:ATP-Binding Cassette Transporters / Q000502:physiology*; D017360:Arabidopsis / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000502:physiology*; D001483:Base Sequence; D017931:DNA Primers; D007210:Indoleacetic Acids / Q000502:physiology*; D018517:Plant Roots / Q000502:physiology*
D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; D017931:DNA Primers; D007210:Indoleacetic Acids