Human centromere protein B induces translational positioning of nucleosomes on alpha-satellite sequences.
The human centromere proteins A (CENP-A) and B (CENP-B) are the fundamental centromere components of chromosomes. CENP-A is the centromere-specific histone H3 variant, and CENP-B specifically binds a 17-base pair sequence (the CENP-B box), which appears within every other alpha-satellite DNA repeat. In the present study, we demonstrated centromere-specific nucleosome formation in vitro with recombinant proteins, including histones H2A, H2B, H4, CENP-A, and the DNA-binding domain of CENP-B. The CENP-A nucleosome wraps 147 base pairs of the alpha-satellite sequence within its nucleosome core particle, like the canonical H3 nucleosome. Surprisingly, CENP-B binds to nucleosomal DNA when the CENP-B box is wrapped within the nucleosome core particle and induces translational positioning of the nucleosome without affecting its rotational setting. This CENP-B-induced translational positioning only occurs when the CENP-B box sequence is settled in the proper rotational setting with respect to the histone octamer surface. Therefore, CENP-B may be a determinant for translational positioning of the centromere-specific nucleosomes through its binding to the nucleosomal CENP-B box.
Tanaka
Yoshinori
Y
Protein Research Group, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.
Tachiwana
Hiroaki
H
Yoda
Kinya
K
Masumoto
Hiroshi
H
Okazaki
Tsuneko
T
Kurumizaka
Hitoshi
H
Yokoyama
Shigeyuki
S
D001324:Autoantigens / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D002503:Centromere / Q000648:ultrastructure; D000076247:Centromere Protein A; D051936:Centromere Protein B / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000737:chemistry; D004276:DNA, Satellite; D003850:Deoxyribonuclease I / Q000737:chemistry; D006657:Histones / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008836:Micrococcal Nuclease / Q000378:metabolism; D008957:Models, Genetic; D008958:Models, Molecular; D009154:Mutation; D009707:Nucleosomes / Q000378:metabolism*; D011485:Protein Binding; D014176:Protein Biosynthesis; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry
D001324:Autoantigens; C000619700:CENPA protein, human; D000076247:Centromere Protein A; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D004276:DNA, Satellite; D006657:Histones; D009707:Nucleosomes; D011994:Recombinant Proteins; D004247:DNA; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease