title: [The effects of Mcl-1 gene on ATRA-resistant HL-60 cell].
abstract: OBJECTIVE
  To investigate the role of Mcl-1 gene in resistance of all-trans retinoic acid (ATRA) of leukemia cells.
  
  
  METHODS
  Long-term, intermittent and repetitive exposure of HL-60 cells to ATRA was used to establish a multidrug-resistance cell line (HL-60/ATRA). HL-60/ATRA cells were transfected with Mcl-1 small interference RNA (siRNA) by Lipofectamine 2000. Western blot was used to detect the expression of Mcl-1. The proliferation, apoptosis and differentiation were evaluated by MTT assay, in situ nick end-labeling (TUNEL) and NBT assay, respectively.
  
  
  RESULTS
  The HL-60/ATRA could keep its undifferentiated and proliferative status to a high concentration of ATRA (100 nmol/L) with highly expressed Mcl-1 protein (relative grey scale 0.624 +/- 0.127). Mcl-1 gene knockdown by siRNA (relative grey scale 0.267 +/- 0.086) could reverse the resistance of ATRA of HL-60/ATRA by inhibiting proliferation, and inducing differentiation and apoptosis [apoptosis rate (18.5 +/- 4.5)%].
  
  
  CONCLUSIONS
  Mcl-1 gene might be involved in ATRA resistance in HL-60 cells and inhibiting its expression could be a new approach to ATRA resistance reversion.
authors.affiliation: Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Jin-rong
authors.initials: JR
authors.lastname: Fu
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Liu
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Zhou
authors.forename: Han-ying
authors.initials: HY
authors.lastname: Sun
authors.forename: Miao
authors.initials: M
authors.lastname: Zheng
authors.forename: Mei
authors.initials: M
authors.lastname: Huang
authors.forename: Chun-rui
authors.initials: CR
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Ran
authors.forename: Li
authors.initials: L
authors.lastname: Luo
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering; D014212:Tretinoin