title: Apoptosis role of FAS/FAS ligand system in the regulation of myelopoiesis.
abstract: OBJECTIVE
  CD34+ cells and colony forming unit-granulocyte and macrophage (CFU-GM) from human bone marrow were used to investigate the role of Fas/FasL system in the regulation of myelopoiesis.
  
  
  METHODS
  Fas and FasL expression in CD34+ cells and in day 14 CFU-GM were measured by RT-PCR and immunofluorescence respectively. The functional assays for the CFU-GM were measured by a standard colony assay and the proliferative capacity of CFU-GM was measured by replating the primary colony and observing the secondary colony formation. Human marrow cells were treated with IETD (caspase-8 inhibitor) or anti-Fas CH-11 Mab.
  
  
  RESULTS
  Treatment with the CFU-GM with IETD significantly increased, the proliferative capacity, while anti-Fas CH-11 Mab markedly reduced it. Fas and FasL expression were demonstrated using RT-PCR and immunofluorescence respectively.
  
  
  CONCLUSIONS
  Fas, FasL, and caspase activation are likely to play an important role in the regulation of myelopoiesis.
authors.affiliation: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, King Faisal University, Dammam, Saudi Arabia. falenzi@kfu.edu.sa
authors.forename: Faris Q
authors.initials: FQ
authors.lastname: Alenzi
authors.forename: Saleh M
authors.initials: SM
authors.lastname: Al-Ghamdi
authors.forename: Waleed G
authors.initials: WG
authors.lastname: Tamimi
authors.forename: Abdulaziz M
authors.initials: AM
authors.lastname: Al-Sebiany
authors.forename: Iman M
authors.initials: IM
authors.lastname: El-Nashar
authors.forename: Iman
authors.initials: I
authors.lastname: El-Tounsi
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: Bamaga
authors.forename: Maher M
authors.initials: MM
authors.lastname: Al-Enazi
authors.forename: Ali S
authors.initials: AS
authors.lastname: Al-Amri
authors.forename: Iman H
authors.initials: IH
authors.lastname: Al-Sheikh
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D053222:Fas Ligand Protein; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D038042:Myelopoiesis / Q000502:physiology*; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D048069:Tumor Necrosis Factors / Q000378:metabolism*; D019014:fas Receptor
chemical_list: D018952:Antigens, CD34; C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D018124:Receptors, Tumor Necrosis Factor; D048069:Tumor Necrosis Factors; D019014:fas Receptor