title: Rat renal glucose transporter SGLT1 exhibits zonal distribution and androgen-dependent gender differences.
abstract: SGLT1 (SLC5A1) mediates a part of glucose and galactose reabsorption in the mammalian proximal tubule (PT), but the detailed localization of the transporter along the tubule is still disputable. Here, we used several methods to localize rat SGLT1 (rSGLT1) in the kidneys of intact and variously treated male (M) and female (F) rats. In immunoblots of isolated cortical (C) and outer stripe (OS) brush-border membranes (BBM), a peptide-specific polyclonal antibody for rSGLT1 labeled a sharp inzone-, and gender-dependent approximately 40-kDa protein and a broad approximately 75-kDa band that exhibited strong zonal (OS > C) and gender differences (F > M). In tissue cryosections, the antibody strongly stained BBM of the S3 PT segments in the OS and medullary rays (F > M) and smooth muscles of the blood vessels and renal capsule (F approximately M) and weakly stained the apical domain of other PT segments in the C (F approximately M). The phlorizin-sensitive uptake of d-[(3)H]galactose in BBM vesicles, as well as the tissue abundance of rSGLT1-specific mRNA, matched the immunoblotting data related to the 75-kDa protein and the immunostaining in S3, proving zonal and gender differences in the functional transporter. Ovariectomy had no effect, castration upregulated, whereas treatment of castrated rats with testosterone, but not with estradiol or progesterone, downregulated the 75-kDa protein and the immunostaining in S3. We conclude that in the rat kidney, the expression of SGLT1 is represented by a 75-kDa protein localized largely in the PT S3 segments, where it exhibits gender differences (F > M) at both the protein and mRNA levels that are caused by androgen inhibition.
authors.affiliation: Unit of Molecular Toxicology, Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10001 Zagreb, Croatia. sabolic@imi.hr
authors.forename: Ivan
authors.initials: I
authors.lastname: Sabolić
authors.forename: Mario
authors.initials: M
authors.lastname: Skarica
authors.forename: Valentin
authors.initials: V
authors.lastname: Gorboulev
authors.forename: Marija
authors.initials: M
authors.lastname: Ljubojević
authors.forename: Daniela
authors.initials: D
authors.lastname: Balen
authors.forename: Carol M
authors.initials: CM
authors.lastname: Herak-Kramberger
authors.forename: Hermann
authors.initials: H
authors.lastname: Koepsell
mesh_terms: D000728:Androgens / Q000502:physiology; D000818:Animals; D002369:Castration; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015151:Immunoblotting; D007678:Kidney Glomerulus / Q000737:chemistry* / Q000502:physiology; D007687:Kidney Tubules, Proximal / Q000737:chemistry* / Q000502:physiology; D008297:Male; D010052:Ovariectomy; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors; D051269:Sodium-Glucose Transporter 1 / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology
chemical_list: D000728:Androgens; D012333:RNA, Messenger; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1