Classification of wheat low-molecular-weight glutenin subunit genes and its chromosome assignment by developing LMW-GS group-specific primers.
On the basis of sequence analysis, 69 known low-molecular-weight glutenin subunit (LMW-GS) genes were experimentally classified into nine groups by the deduced amino acid sequence of the highly conserved N-terminal domain. To clarify the chromosomal locations of these groups, 11 specific primer sets were designed to carry out polymerase chain reactions (PCR) with the genomic DNA of group 1 ditelosomic lines of Chinese Spring, among which nine primer sets proved to be LMW-GS group-specific. Each group of LMW-GS genes was specifically assigned on a single chromosome arm and hence to a specific locus. Therefore, these results provided the possibility to predict the chromosome location of a new LMW-GS gene based on its deduced N-terminal sequence. The validity of the classification was confirmed by the amplifications in 27 diploid wheat and Aegilops accessions. The length polymorphisms of LMW-GS genes of groups 1 and 2, and groups 3 and 4.1 were detected in diploid A-genome and S-genome accessions, respectively. The diploid wheat and Aegilops species could be used as valuable resources of novel allele variations of LMW-GS gene in the improvement of wheat quality. The nine LMW-GS group-specific primer sets could be utilized to select specific allele variations of LMW-GS genes in the marker-assisted breeding.
Long
Hai
H
Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan 611830, China.
Wei
Yu-Ming
YM
Yan
Ze-Hong
ZH
Baum
Bernard
B
Nevo
Eviatar
E
Zheng
You-Liang
YL
D001947:Breeding / Q000379:methods; D002874:Chromosome Mapping / Q000379:methods*; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005983:Glutens / Q000235:genetics*; D008957:Models, Genetic; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D021122:Protein Subunits / Q000145:classification / Q000235:genetics*; D013045:Species Specificity; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers; D021122:Protein Subunits; D005983:Glutens; C037302:glutenin