Production of alkaline protease from an alkaliphilic actinomycete.
The repression of alkaline protease synthesis from alkaliphilic actinomycete was studied by using glucose, peptone, yeast extract, KH2PO4 and amino acids; tyrosine, tryptophan, lysine, and arginine. There was a critical limit of stimulation of enzyme production by these components. Crude components such as molasses, wheat flour, and wheat bran were found to be effective for growth and enzyme production. The high level of enzyme production using agro-industrial by-products is commercially significant due to cheap nature of these sources. The findings are quite attractive, as only few actinomycetes, particularly alkaliphilic ones, have so far been explored for their enzymatic potential and regulation of enzyme synthesis.
Mehta
V J
VJ
Microbiology Unit, Department of Biosciences, Saurashtra University, Rajkot 360 005, Gujarat, India.
Thumar
J T
JT
Singh
S P
SP
D039903:Actinobacteria / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D050260:Carbohydrate Metabolism; D003470:Culture Media / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D005433:Flour; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008965:Molasses; D009584:Nitrogen / Q000494:pharmacology; D010461:Peptones / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology; D012988:Soil Microbiology; D013379:Substrate Specificity; D014908:Triticum / Q000737:chemistry / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D003470:Culture Media; D004043:Dietary Fiber; D010461:Peptones; D010710:Phosphates; D010450:Endopeptidases; C087822:alkaline protease; D005947:Glucose; D009584:Nitrogen