Hepatoprotective effect of green tea (Camellia sinensis) extract against tamoxifen-induced liver injury in rats.
Tamoxifen citrate (TAM), is widely used for treatment of breast cancer. It showed a degree of hepatic carcinogenesis. The purpose of this study was to elucidate the antioxidant capacity of green tea (Camellia sinensis) extract (GTE) against TAM-induced liver injury. A model of liver injury in female rats was done by intraperitoneal injection of TAM in a dose of 45mg Kg(-1) day(-1), i.p. for 7 successive days. GTE in the concentration of 1.5 %, was orally administered 4 days prior and 14 days after TAM-intoxication as a sole source of drinking water. The antioxidant flavonoid; epicatechin (a component of green tea) was not detectable in liver and blood of rats in either normal control or TAM-intoxicated group, however, TAM intoxication resulted in a significant decrease of its level in liver homogenate of tamoxifenintoxicated rats. The model of TAM-intoxication elicited significant declines in the antioxidant enzymes (glutathione-S-transferase,glutathione peroxidase, superoxide dismutase and catalase) and reduced glutathione concomitant with significant elevations in TBARS (thiobarbituric acid reactive substance) and liver transaminases; sGPT (serum glutamate pyruvate transaminase) and sGOT (serum glutamate oxaloacetate transaminase) levels. The oral administration of 1.5 % GTE to TAM-intoxicated rats, produced significant increments in the antioxidant enzymes and reduced glutathione concomitant with significant decrements in TBARS and liver transaminases levels. The data obtained from this study speculated that 1.5 % GTE has the capacity to scavenge free radical and can protect against oxidative stress induced by TAM intoxication. Supplementation of GTE could be useful in alleviating tamoxifen-induced liver injury in rats.
El-Beshbishy
Hesham A
HA
Biochemistry Department, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo, Egypt. Hesham_Elbeshbishy@hotmail.com
D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000633:toxicity*; D000975:Antioxidants / Q000378:metabolism; D028241:Camellia sinensis / Q000737:chemistry; D002392:Catechin / Q000032:analysis; D056486:Chemical and Drug Induced Liver Injury; D005260:Female; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology*; D008107:Liver Diseases / Q000517:prevention & control*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D013629:Tamoxifen / Q000633:toxicity*; D013662:Tea / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D018931:Antineoplastic Agents, Hormonal; D000975:Antioxidants; D010936:Plant Extracts; D013662:Tea; D017392:Thiobarbituric Acid Reactive Substances; D013629:Tamoxifen; D002392:Catechin