Comparative nucleotide sequence analysis of polymorphic variable-number tandem-repeat Loci in Mycobacterium ulcerans.
We analyzed a set of variable-number tandem-repeat (VNTR) loci to assess their nucleotide sequence diversity in isolates of three Mycobacterium ulcerans genotypes. Sequence variants in two loci resulted in intraspecies resolution of Southeast Asian and Asian genotypes in contrast to a homogenous sequence composition among African isolates. Nucleotide sequence polymorphism in repeat units can enhance discrimination of VNTR loci.
Ablordey
Anthony
A
Mycobacteriology Unit, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium.
Hilty
Markus
M
Stragier
Pieter
P
Swings
Jean
J
Portaels
Françoise
F
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D019911:Mycobacterium ulcerans / Q000145:classification* / Q000235:genetics*; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D013045:Species Specificity