title: Polyphenol levels in human urine after intake of six different polyphenol-rich beverages.
abstract: Dietary polyphenols are suggested to participate in the prevention of CVD and cancer. It is essential for epidemiological studies to be able to compare intake of the main dietary polyphenols in populations. The present paper describes a fast method suitable for the analysis of polyphenols in urine, selected as potential biomarkers of intake. This method is applied to the estimation of polyphenol recovery after ingestion of six different polyphenol-rich beverages. Fifteen polyphenols including mammalian lignans (enterodiol and enterolactone), several phenolic acids (chlorogenic, caffeic, m-coumaric, gallic, and 4-O-methylgallic acids), phloretin and various flavonoids (catechin, epicatechin, quercetin, isorhamnetin, kaempferol, hesperetin, and naringenin) were simultaneously quantified in human urine by HPLC coupled with electrospray ionisation mass-MS (HPLC-electrospray-tandem mass spectrometry) with a run time of 6 min per sample. The method has been validated with regard to linearity, precision, and accuracy in intra- and inter-day assays. It was applied to urine samples collected from nine volunteers in the 24 h following consumption of either green tea, a grape-skin extract, cocoa beverage, coffee, grapefruit juice or orange juice. Levels of urinary excretion suggest that chlorogenic acid, gallic acid, epicatechin, naringenin or hesperetin could be used as specific biomarkers to evaluate the consumption of coffee, wine, tea or cocoa, and citrus juices respectively.
authors.affiliation: Unité des Maladies Métaboliques et Micronutrients, INRA, 63122 Saint-Genès-Champanelle, France.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ito
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Gonthier
authors.forename: Claudine
authors.initials: C
authors.lastname: Manach
authors.forename: Christine
authors.initials: C
authors.lastname: Morand
authors.forename: Louise
authors.initials: L
authors.lastname: Mennen
authors.forename: Christian
authors.initials: C
authors.lastname: Rémésy
authors.forename: Augustin
authors.initials: A
authors.lastname: Scalbert
mesh_terms: D000328:Adult; D001628:Beverages; D015415:Biomarkers / Q000652:urine; D002099:Cacao; D002110:Caffeine; D002138:Calibration; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D032083:Citrus paradisi; D032084:Citrus sinensis; D003069:Coffee; D004032:Diet; D005260:Female; D005419:Flavonoids / Q000008:administration & dosage* / Q000737:chemistry / Q000652:urine*; D006801:Humans; D008297:Male; D010636:Phenols / Q000008:administration & dosage* / Q000737:chemistry / Q000652:urine*; D059808:Polyphenols; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D018709:Statistics, Nonparametric; D013662:Tea; D013805:Theobromine; D013997:Time Factors
chemical_list: D015415:Biomarkers; D003069:Coffee; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013662:Tea; D002110:Caffeine; D013805:Theobromine