title: [Flow cytometry-pathology combined study of breast cancer].
abstract: OBJECTIVE
  To study the relation between histopathologic grading and some of the cytogenetic and molecular biology characteristics of breast cancer.
  
  
  METHODS
  On the basis of estrogen receptor (ER) expression, DNA content, S-phase fraction (SPF), bcl-2 and mutant p53 protein (mtp53) expression were examined by FCM in 121 breast cancer patients. In 66 patients with invasive ductal breast cancer, histopathologic grading was also examined.
  
  
  RESULTS
  The aneuploidy rate and DNA index (DI) were significantly different in grade I, II and III breast cancer. SPF and mtp53 expression significantly increased with increase in histopathologic grading (P < 0.05), but bcl-2 did not show this trend. SPF and mtp53 expression were significantly more in breast cancer with negative ER than in those with positive ER (P < 0.05). Again, no such differences in bcl-2 regardless of ER expression. Correlations existed between DI vs SPF, DI vs mtp53, and SPF vs mtp53 expressions (P < 0.01) but bcl-2 did not correlate with any one of them.
  
  
  CONCLUSIONS
  Cytogenetic and molecular biology studies on the basis of histopathologic grading may provide more information in prognostic prediction of breast cancer.
authors.affiliation: Department of Breast Cancer, Shandong Tumor Hospital & Institute, Jinan 250117, China. Drzhiyongyu@yahoo.com
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Yu
authors.forename: Jin-ming
authors.initials: JM
authors.lastname: Yu
authors.forename: Tai-huang
authors.initials: TH
authors.lastname: Wu
authors.forename: Pei-ying
authors.initials: PY
authors.lastname: Zhuo
authors.forename: Sheng-fang
authors.initials: SF
authors.lastname: Wang
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.forename: Bao-yu
authors.initials: BY
authors.lastname: Lü
authors.forename: Yan-bing
authors.initials: YB
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D000782:Aneuploidy; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics; D016196:S Phase; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm; D019253:Proto-Oncogene Proteins c-bcl-2; D011960:Receptors, Estrogen; D016159:Tumor Suppressor Protein p53