[Screening and identification of phage-displayed polypeptides specifically binding to human gastric cancer with high metastatic potential to peritoneum].
OBJECTIVE
By means of phage-display technique, to screen polypeptides that specifically bind to human gastric cancer with high metastatic potential to peritoneum.


METHODS
Two human gastric cancer cell lines were used: GC9811-P with high metastatic potential to peritoneum and its wild type parental GC9811, to carry out subtractive screening with a phage display-12 peptide library.


RESULTS
After three rounds of screening, 40 phage clones bond to GC9811-P cells were randomly selected. When injected into the peritoneal cavity of nude mice, 6 of the 40 clones did not bind to mouse peritoneum as examined by immunohistochemical staining. They were considered to be capable of binding specifically to GC9811-P cells. Sequence analysis revealed two different exogenous peptides: TLNINRLILPRT and SMSI(X)SPYI(XXX).


CONCLUSIONS
Two peptides have been obtained that specifically bind to a gastric cancer cell variant GC9811-P, which easily disseminates to the peritoneum. Whether or not they could block GC9811-P metastasis to peritoneum in vivo remains to be determined.
Zhang
Ke-dong
KD
Affiliated Hospital, Ningxia Medical College, Yinchuan 750004, China.
Guo
Xin-ning
XN
Yang
Li
L
Zhang
Dong-tao
DT
Bai
Fei-hu
FH
Jiang
Hai-ping
HP
Zhai
Hui-hong
HH
Nie
Yong-zhan
YZ
Wu
Kai-chun
KC
Fan
Dai-ming
DM
D000818:Animals; D001665:Binding Sites; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D010534:Peritoneal Neoplasms / Q000556:secondary*; D040081:Protein Array Analysis / Q000379:methods; D011485:Protein Binding; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*
D019151:Peptide Library; D010455:Peptides