[Qualitative and quantitative methods of betulonic acid in fruits of Liquidambar formosana].
OBJECTIVE
To develop the identification and assay methods for betulonic acid in fruits of Liquidambar formosana.


METHODS
TLC method was used for identification with silica gel G plate and petroleum ether(60-90 degrees C)-acetone (17:3) as a developing solvent. Betulonic acid in ethanol extract was separated on the ODS column with methanol-water-glacial acetic acid (87:13:0.1) as mobile phase. Flow-rate was 1.0 mL x min(-1), and column temperature 35 degrees C. ELSD drift-tube temperature was 82 degrees C, and gas flow 1.25 L x min(-1).


RESULTS
The qualitative method is repeatable. Betulonic acid in ethanol extracts is well separated, relationship of logarithms of injection amount and peak area is linear (r = 0.9997) within the range of 0.65-3.25 microg. The average recovery is 98.0% and RSD of repeatability is 2.5%. 11 crude drugs purchased from different areas in the country were identified and quantified with the methods.


CONCLUSIONS
The methods and data could be used for quality control of fruits of L. formosana.
Zhang
Qi-Wei
QW
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Zhang
Yong-Xin
YX
Sun
Yu-Ru
YR
Zhang
Dong
D
D002855:Chromatography, Thin Layer / Q000379:methods; D005638:Fruit / Q000737:chemistry; D031317:Liquidambar / Q000737:chemistry*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D000094062:Betulinic Acid
D009828:Oleanolic Acid; C118308:betulonic acid; D000094062:Betulinic Acid