title: Determination of protein-derived epitopes by mass spectrometry.
abstract: Mass spectrometry has evolved as a technique suitable for the characterization of peptides and proteins beyond their linear sequence. The advantages of mass spectrometric sample analysis are high sensitivity, high mass accuracy, rapid analysis time and low sample consumption. In epitope mapping, the molecular structure of an antigen (the epitope or antigenic determinant) that interacts with the paratope (recognition surface) of the antibody is identified. To obtain information on linear, conformational and/or discontinuous epitopes, various approaches have been developed in conjunction with mass spectrometry. These methods include limited proteolysis and epitope footprinting, epitope excision and epitope extraction for linear epitopes and probing the surface accessibility of residues by differential chemical modifications of specific amino acid side chains or by differential hydrogen/deuterium exchange of the protein backbone amides for conformational and discontinuous epitopes. Epitope mapping by mass spectrometry is applicable in basic biochemical research and, with increasing robustness, should soon find its implementation in routine clinical diagnosis.
authors.affiliation: Laboratory of Structural Biology, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA. hagerbr1@niehs.nih.gov
authors.forename: Christine
authors.initials: C
authors.lastname: Hager-Braun
authors.forename: Kenneth B
authors.initials: KB
authors.lastname: Tomer
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology*; D013058:Mass Spectrometry; D008958:Models, Molecular; D011506:Proteins / Q000737:chemistry*
chemical_list: D000936:Antigen-Antibody Complex; D000939:Epitopes; D011506:Proteins