title: [Expression of urotensin II and G-protein coupled receptor 14 mRNA in human pheochromocytoma tissues].
abstract: OBJECTIVE
  To investigate the expression of urotensin II (U II) and G-protein coupled receptor 14 (GPR14) mRNA in human pheochromocytoma tissues.
  
  
  METHODS
  Total RNA from normal adrenal and pheochromocytoma tissues was extracted. The reverse transcription-polymerase chain reaction method was used to evaluate the levels of U II and GPR14 mRNA expression in human pheochromocytoma tissues.
  
  
  RESULTS
  There was no significant difference of U II and GPR14 mRNA expression between normal adrenal cortex and medulla. The expression of U II and GPR14 mRNA in pheochromocytoma was significantly lower than that in normal adrenal cortex and medulla (P < 0.05). The expression of GPR14 mRNA in adrenal pheochromocytomas was significantly lower than that of extra-adrenal pheochromocytomas (P < 0.05).
  
  
  CONCLUSIONS
  U II and GPR14 may play a role in the pathogenesis and hypertension regulating of pheochromocytoma.
authors.affiliation: Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Liu
authors.forename: Zheng-Pei
authors.initials: ZP
authors.lastname: Zeng
authors.forename: Han-Zhong
authors.initials: HZ
authors.lastname: Li
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Fan
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Liu
authors.forename: An-Li
authors.initials: AL
authors.lastname: Tong
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
mesh_terms: D000302:Adrenal Cortex / Q000378:metabolism; D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000313:Adrenal Medulla / Q000378:metabolism; D006801:Humans; D010673:Pheochromocytoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics; D014579:Urotensins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C121697:UTS2R protein, human; D014579:Urotensins; C029677:urotensin II