Surface composition of myrmecophilic plants: cuticular wax and glandular trichomes on leaves of Macaranga tanarius.
Primary plant surfaces, covered with cuticles consisting of cutin and waxes, are important substrates for interaction with insects. The composition of leaf surfaces of the myrmecophilic plant Macaranga tanarius was studied. The prenylated flavanone nymphaeol-C was identified in surface extracts and was localized exclusively in glandular trichomes on the abaxial leaf side. The epidermal pavement cells surrounding these trichomes were covered with a smooth film of epicuticular wax from which few small wax crystals protruded. The epicuticular wax amounted to approximately 8 microg cm(-2), corresponding to 85% of the wax load on the adaxial as well as the abaxial leaf sides. The epicuticular wax mixtures from both leaf surfaces contained more than 70% primary alcohols, 14% fatty acids, 2% aldehydes, and traces of alkyl acetates, with chain lengths ranging from C(20) to C(38). In contrast, the intracuticular wax layer was largely dominated by triterpenoid alcohols alpha-amyrin, beta-amyrin, and lupeol. Consequently, these characteristic compounds are not available for direct contact with insects on the plant surface.
Guhling
Ortwin
O
Julius-von-Sachs Institut für Biowissenschaften, Universität Würzburg, Würzburg, Germany.
Kinzler
Christian
C
Dreyer
Michael
M
Bringmann
Gerhard
G
Jetter
Reinhard
R
D000085:Acetates / Q000737:chemistry / Q000302:isolation & purification; D000438:Alcohols / Q000737:chemistry / Q000302:isolation & purification; D000447:Aldehydes / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D019665:Euphorbiaceae / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry / Q000302:isolation & purification; D007313:Insecta / Q000502:physiology*; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D053978:Pentacyclic Triterpenes; D018515:Plant Leaves / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification; D014885:Waxes / Q000737:chemistry* / Q000378:metabolism; D000094042:Lupanes
D000085:Acetates; D000438:Alcohols; D000447:Aldehydes; D005227:Fatty Acids; D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D013729:Terpenes; D014315:Triterpenes; D014885:Waxes; C036380:beta-amyrin; C010480:lupeol; D000094042:Lupanes