title: Reference maps of mouse serum acute-phase proteins: changes with LPS-induced inflammation and apolipoprotein A-I and A-II transgenes.
abstract: We present reference maps of the mouse serum proteome (run under reducing and non-reducing conditions), from control animals, from mice injected with lipopolysaccharide (LPS) to induce systemic inflammation, and from mice transgenic for human apolipoproteins A-I and A-II. Seventy-seven spots/spot chains from the reducing gels were identified by HPLC MS/MS, representing 28 distinct proteins, including a species-specific protease inhibitor, contrapsin, and high levels of carboxylesterase. The concentrations of acute-phase reactants were monitored for 96 h after LPS challenge. The greatest changes (four-fold 48 h after LPS administration) were observed for haptoglobin and hemopexin. Orosomucoid/alpha(1)-acid glycoprotein and apolipoprotein A-I increased steadily, to 50-60% above baseline at 96 h from stimulation. In mice transgenic for human apolipoprotein A-I the levels of expression of orosomucoid/alpha(1)-acid glycoprotein, alpha(1)-macroglobulin, esterase, kininogen and contrapsin were altered compared to knockout mice lacking apolipoprotein A-I. In contrast, except for the presence of apolipoprotein A-II, no statistically significant difference was observed in mice transgenic for human apolipoprotein A-II.
authors.affiliation: Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College London, UK.
authors.forename: Robin
authors.initials: R
authors.lastname: Wait
authors.forename: Giulia
authors.initials: G
authors.lastname: Chiesa
authors.forename: Cinzia
authors.initials: C
authors.lastname: Parolini
authors.forename: Ingrid
authors.initials: I
authors.lastname: Miller
authors.forename: Shajna
authors.initials: S
authors.lastname: Begum
authors.forename: Daniela
authors.initials: D
authors.lastname: Brambilla
authors.forename: Lara
authors.initials: L
authors.lastname: Galluccio
authors.forename: Rossana
authors.initials: R
authors.lastname: Ballerio
authors.forename: Ivano
authors.initials: I
authors.lastname: Eberini
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Gianazza
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism*; D000210:Acute-Phase Reaction; D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics*; D016633:Apolipoprotein A-II / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006242:Haptoglobins / Q000378:metabolism; D006466:Hemopexin / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008822:Mice, Transgenic; D009961:Orosomucoid / Q000378:metabolism; D012016:Reference Values
chemical_list: D000209:Acute-Phase Proteins; D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D006242:Haptoglobins; D008070:Lipopolysaccharides; D009961:Orosomucoid; D006466:Hemopexin