[Establishment and characterization of human inflammatory breast carcinoma neoplasm transplantation in nude mice].
OBJECTIVE
To establish and characterize human inflammatory breast cancer xenograft in nude mice.


METHODS
Animal studies, Northern and Western blot, zymograms and immunohistochemistry were used in our studies.


RESULTS
Human transplantable inflammatory carcinoma were established in nude mice. This xenograft as its human counterpart exhibited striking erythema of the overlying skin, lympho-vascular invasion and other similar biological characteristics. It also exhibited lung metastasis 4-6 weeks after growth. This xenograft was ER, PR, Her-2/neu negative and p53, EGFR positive. Comparative studies of inflammatory breast cancer xenograft with non-inflammatory xenograft indicated 10-20 fold overexpression of E-cadherin and MUC1.


CONCLUSIONS
This human inflammatory breast carcinoma xenograft will provide an excellent animal model to allow us to further dissect out both the upstream regulatory machinery and downstream effector molecules responsible for the inflammatory carcinoma phenotype.
Shao
Z
Z
Department of Surgery, Laboratory for Molecular Biology, Shanghai Cancer Hospital, Medical Center of Fudan University, Shanghai 200032, China.
Liu
G
G
Ding
J
J
D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D015820:Cadherins / Q000378:metabolism; D005260:Female; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009077:Mucins / Q000378:metabolism; D009368:Neoplasm Transplantation; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D015820:Cadherins; D009077:Mucins; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D016159:Tumor Suppressor Protein p53