title: Spatial and temporal localization of FGF-2 and VEGF in healing tooth extraction sockets in a rabbit model.
abstract: OBJECTIVE
  The purpose of this study was to test the hypothesis that spatial and temporal localization of growth factors FGF-2 and VEGF in a rabbit tooth extraction socket model correlate with the histologic events of healing.
  
  
  METHODS
  Twenty-four male New Zealand white rabbits divided into 8 groups of 3 were used in the study. Incisor teeth were extracted from both jaws and the healing extraction socket with surrounding jaw bone was harvested at 48 hours, 4 days, 1, 2, 4, 8, 12, and 16 weeks. Tissues were fixed, decalcified, and processed for hematoxylin-eosin and immunohistochemical staining. The sections were stained to detect FGF-2 and VEGF. The stained sections were then imaged and an automated computer program was used to detect the brown diaminobenzidine stain that represented the growth factors of interest. Data was obtained in the form of percentage area and intensity of stain and analyzed using the analysis of variance (ANOVA - Tukey Kramer and Scheffe's post-test).
  
  
  RESULTS
  Spatial and temporal differences in localization of FGF-2 and VEGF were observed across all time frames in both jaws. Statistically significant differences in percentage area and intensity of brown diaminobenzidine stain were seen temporally between FGF-2 and VEGF (P < .05).
  
  
  CONCLUSIONS
  The results of this study showed positive correlation of histologic events to spatial and temporal localization of FGF-2 and VEGF in a rabbit tooth extraction model.
authors.affiliation: Department of Oral & Maxillofacial Surgery, University of Texas Health Science Center at Houston, Houston, TX, USA. zahidl@yahoo.com
authors.forename: Zahid
authors.initials: Z
authors.lastname: Lalani
authors.forename: Mark
authors.initials: M
authors.lastname: Wong
authors.forename: Eric M
authors.initials: EM
authors.lastname: Brey
authors.forename: Antonios G
authors.initials: AG
authors.lastname: Mikos
authors.forename: P J
authors.initials: PJ
authors.lastname: Duke
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Miller
authors.forename: Carol
authors.initials: C
authors.lastname: Johnston
authors.forename: Dina
authors.initials: D
authors.lastname: Montufar-Solis
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D023421:Models, Animal; D011817:Rabbits; D018709:Statistics, Nonparametric; D014018:Tissue Distribution; D014081:Tooth Extraction; D020390:Tooth Socket / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2