title: Application of immobilized metal affinity chromatography in proteomics.
abstract: It has been proved that the progress of proteomics is mostly determined by the development of advanced and sensitive protein separation technologies. Immobilized metal affinity chromatography (IMAC) is a powerful protein fractionation method used to enrich metal-associated proteins and peptides. In proteomics, IMAC has been widely employed as a prefractionation method to increase the resolution in protein separation. The combination of IMAC with other protein analytical technologies has been successfully utilized to characterize metalloproteome and post-translational modifications. In the near future, newly developed IMAC integrated with other proteomic methods will greatly contribute to the revolution of expression, cell-mapping and structural proteomics.
authors.affiliation: Department of Chemistry, University of Hong Kong, Pokfulam, Hong Kong. sunxs@hkusua.hku.hk
authors.forename: Xuesong
authors.initials: X
authors.lastname: Sun
authors.forename: Jen-Fu
authors.initials: JF
authors.lastname: Chiu
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: He
mesh_terms: D000818:Animals; D005591:Chemical Fractionation; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D008670:Metals / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
chemical_list: D008670:Metals; D011506:Proteins