title: Antinociceptive anti-inflammatory effect of Monotropein isolated from the root of Morinda officinalis.
abstract: The root of Morinda officinalis (Rubiaceae) is used to treat rheumatoid arthritis and impotence in the traditional Oriental medicine. To identify the antinociceptive anti-inflammatory components of this crude drug, we adopted an activity-directed fractionation approach. The active fraction of the BuOH extract of M. officinalis root was subjected to silica gel and ODS column chromatography to yield two diterpenes, compounds 1 and 2 and these were identified as monotropein and deacetylasperulosidic acid, respectively. The iridoid glycoside, monotropein, was tested for its anti-inflammatory antinociceptive effects using hot plate- and writhing antinociceptive assays and by using carrageenan-induced anti-inflammatory assays in mice and rats. Pretreatment with monotropein (at 20, 30 mg/kg/d, p.o.) significantly reduced stretching episodes and prolonged action time in mice. It also significantly reduced acute paw edema by carrageenan in rats. These results indicate that monotropein contributes to the antinociceptive and anti-inflammatory action of Morinda officinalis root.
authors.affiliation: College of Pharmacy, Daeyeon-Dong, Kyungsung University; Busan 608-736, Korea.
authors.forename: Jongwon
authors.initials: J
authors.lastname: Choi
authors.forename: Kyung-Tae
authors.initials: KT
authors.lastname: Lee
authors.forename: Moo-Young
authors.initials: MY
authors.lastname: Choi
authors.forename: Jung-Hwan
authors.initials: JH
authors.lastname: Nam
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Jung
authors.forename: Sun-Kyu
authors.initials: SK
authors.lastname: Park
authors.forename: Hee-Juhn
authors.initials: HJ
authors.lastname: Park
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002850:Chromatography, Gel; D039823:Iridoids / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D032066:Morinda / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013057:Spectrum Analysis
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D039823:Iridoids; C507582:monotropein