Importance of Providencia species as a major cause of travellers' diarrhoea.
In this study the importance of Providencia species as a cause of travellers' diarrhoea was examined using a selective medium developed by the authors. Providencia species could easily be distinguished from other enteric pathogens by the colour of the colonies obtained. Nine strains of Providencia alcalifaciens, nine of Providencia rettgeri and five of Providencia stuartii were isolated from 130 specimens, representing a surprisingly high incidence of infection compared with other pathogens isolated on SS agar and TCBS agar. Patients infected with P. rettgeri complained of abdominal pain, as for other Providencia species, but also of vomiting, which is rather characteristic of P. rettgeri infection. To analyse the pathogenicity of these isolates, their invasiveness was examined using Caco-2 cells. Most of the P. rettgeri strains invaded Caco-2 cells. Random amplified polymorphic DNA (RAPD) fingerprinting showed the same profile for two P. rettgeri isolates from individuals travelling in the same tour group. The results show that Providencia species, especially P. rettgeri, might cause diarrhoea, and that these species are important pathogens.
Yoh
Myonsun
M
Center for Emerging Infectious Diseases1 and Department of Bacterial Infections2, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan 3Kansai Airport Quarantine Station, 1 Senshu Kuko Naka, Tajiri-cho, Sennan-gun 549-0011, Japan.
Matsuyama
Junko
J
Center for Emerging Infectious Diseases1 and Department of Bacterial Infections2, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan 3Kansai Airport Quarantine Station, 1 Senshu Kuko Naka, Tajiri-cho, Sennan-gun 549-0011, Japan.
Ohnishi
Motoki
M
Center for Emerging Infectious Diseases1 and Department of Bacterial Infections2, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan 3Kansai Airport Quarantine Station, 1 Senshu Kuko Naka, Tajiri-cho, Sennan-gun 549-0011, Japan.
Takagi
Kazuhiro
K
Center for Emerging Infectious Diseases1 and Department of Bacterial Infections2, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan 3Kansai Airport Quarantine Station, 1 Senshu Kuko Naka, Tajiri-cho, Sennan-gun 549-0011, Japan.
Miyagi
Hirozane
H
Center for Emerging Infectious Diseases1 and Department of Bacterial Infections2, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan 3Kansai Airport Quarantine Station, 1 Senshu Kuko Naka, Tajiri-cho, Sennan-gun 549-0011, Japan.
Mori
Kazuhiro
K
Center for Emerging Infectious Diseases1 and Department of Bacterial Infections2, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan 3Kansai Airport Quarantine Station, 1 Senshu Kuko Naka, Tajiri-cho, Sennan-gun 549-0011, Japan.
Park
Kwon-Sam
KS
Center for Emerging Infectious Diseases1 and Department of Bacterial Infections2, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan 3Kansai Airport Quarantine Station, 1 Senshu Kuko Naka, Tajiri-cho, Sennan-gun 549-0011, Japan.
Ono
Takahiro
T
Center for Emerging Infectious Diseases1 and Department of Bacterial Infections2, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan 3Kansai Airport Quarantine Station, 1 Senshu Kuko Naka, Tajiri-cho, Sennan-gun 549-0011, Japan.
Honda
Takeshi
T
Center for Emerging Infectious Diseases1 and Department of Bacterial Infections2, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan 3Kansai Airport Quarantine Station, 1 Senshu Kuko Naka, Tajiri-cho, Sennan-gun 549-0011, Japan.
D015746:Abdominal Pain; D000293:Adolescent; D000328:Adult; D000368:Aged; D018938:Caco-2 Cells; D003470:Culture Media; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D003967:Diarrhea / Q000382:microbiology* / Q000503:physiopathology; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D008875:Middle Aged; D017720:Molecular Epidemiology; D011532:Providencia / Q000145:classification* / Q000302:isolation & purification* / Q000472:pathogenicity; D019105:Random Amplified Polymorphic DNA Technique; D014195:Travel; D014839:Vomiting
D003470:Culture Media; D004269:DNA, Bacterial