title: Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: control of an outbreak using a new ADSRRS technique.
abstract: Extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae (EPKP) strains are frequently implicated in outbreaks in neonatal units. From April 2002 to January 2003, 149 neonates were colonized/infected with EPKP in the Neonatal Clinic of the Teaching Hospital at the Medical University of Gdańsk, Poland. A novel assay based on suppression of PCR, ADSRRS-fingerprinting, was successfully evaluated for typing EPKP isolates. The results showed that the genotypes of all outbreak-related strains were identical, which suggested that the outbreak originated from a single clone. This conclusion was confirmed by using different methods--RAPD and PFGE. The outbreak was stopped by adopting improved hygiene and instituting outbreak control measures.
authors.affiliation: Department of Microbiology, Gdańsk University of Technology, ul. G. Narutowicza 11/12, 80-952 Gdańsk, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Krawczyk
authors.forename: Alfred
authors.initials: A
authors.lastname: Samet
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Czarniak
authors.forename: Jerzy
authors.initials: J
authors.lastname: Szczapa
authors.forename: Józef
authors.initials: J
authors.lastname: Kur
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016172:DNA Fingerprinting / Q000379:methods; D004196:Disease Outbreaks; D005260:Female; D005838:Genotype; D006784:Hospitals, Teaching; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007235:Infant, Premature, Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000517:prevention & control; D007363:Intensive Care Units, Neonatal; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology / Q000517:prevention & control; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D008297:Male; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases