Micronized purified flavonoid fraction may prevent formation of intraperitoneal adhesions in rats.
OBJECTIVE
To evaluate the efficacy of an anti-inflammatory and capillary regulator drug, micronized purified flavonoid fraction (MPFF), in the prevention of postoperative formation of adhesions.


METHODS
A double-blind, controlled study evaluated the efficacy of MPFF in reducing postoperative adhesion formation in a rat model.


METHODS
Animal care facility of an academic research setting.


METHODS
Thirty Sprague-Dawley female rats randomly divided into three groups.


METHODS
Starting on day of surgery, group 2 rats received oral MPFF (100 mg/kg per day for 7 days). Group 3 rats were intraperitoneally injected with 5 mL of saline (containing 200 mg/kg per day of MPFF for 3 days). Control rats received no medication. A standardized surgical trauma was applied in all animals. Three weeks after surgery, the rats were killed, and the adhesions were scored according to macroscopic and microscopic scales.


METHODS
Postoperative adhesions.


RESULTS
Both oral and intraperitoneal administration of MPFF reduced the scores of adhesions according to macroscopic and microscopic scales. There was no difference between the routes of administration.


CONCLUSIONS
A statistically significant reduction in postoperative formation of adhesions was observed after oral and intraperitoneal administration of MPFF in our experimental animal model. However, further studies are required to reveal its mechanism of action.
Yilmaz
Hatice Gulsen
HG
Department of General Surgery, Faculty of Medicine, University of Dicle, Diyarbakir, Turkey. gyilmaz@dicle.edu.tr
Tacyildiz
Ibrahim Halil
IH
Keles
Celalettin
C
Gedik
Ercan
E
Kilinc
Nihal
N
D000818:Animals; D005591:Chemical Fractionation; D004311:Double-Blind Method; D004353:Drug Evaluation, Preclinical; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification* / Q000627:therapeutic use*; D010532:Peritoneal Diseases / Q000473:pathology / Q000517:prevention & control*; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control*
D005419:Flavonoids; D004364:Pharmaceutical Preparations