title: A novel role for the Bombyx Slbo homologue, BmC/EBP, in insect choriogenesis.
abstract: One previously unidentified cDNA clone coding for a C/EBP factor, BmC/EBP, was isolated from Bombyx mori follicular cells. This is the first time that a C/EBP factor has been isolated and characterized in Lepidoptera. We provide information concerning structural features and developmental specificity, as well as in vitro interaction properties with chorion gene promoter modules. BmC/EBP was capable of effectively recognizing homologous binding sites from chorion gene promoters derived from flies and other moths, despite significant diversity of chorion structure, gene organization, and gene expression profiles. We propose that the relative concentration of BmC/EBP, in relation to its differential binding affinity for promoter cis-elements, results in activation or repression of silkmoth chorion gene expression.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, Panepistimiopolis, Greece.
authors.forename: S
authors.initials: S
authors.lastname: Sourmeli
authors.forename: A
authors.initials: A
authors.lastname: Papantonis
authors.forename: R
authors.initials: R
authors.lastname: Lecanidou
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D012831:Bombyx / Q000254:growth & development*; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000502:physiology*; D002823:Chorion / Q000502:physiology*; D003001:Cloning, Molecular; D004527:Egg Proteins / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007313:Insecta; D008969:Molecular Sequence Data; D006080:Ovarian Follicle / Q000737:chemistry / Q000166:cytology; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D004527:Egg Proteins; C015753:chorion proteins