Natural infection of Viola cornuta (Violaceae) with Cucumber mosaic virus, subgroup I.
Plants of Viola cornuta displaying typical virus symptoms were observed during spring 2003 in a plant nursery in Córdoba, central Argentina. Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 30 nm in diameter. Subsequent serological analysis allowed the identification of the pathogen as a subgroup I strain of Cucumber mosaic virus (CMV). These results were confirmed by antigen capture--reverse transcription--polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. This is the first report of CMV infecting V. cornuta in Argentina.
Arneodo
Joel
J
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET).
de Breuil
Soledad
S
Lenardon
Sergio
S
Conci
Luis
L
D003141:Communicable Diseases; D017799:Cucumovirus / Q000235:genetics*; D004247:DNA / Q000737:chemistry; D007239:Infections; D008854:Microscopy, Electron; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D032413:Viola / Q000821:virology*
D012367:RNA, Viral; D004247:DNA