Prediction of CASP6 structures using automated Robetta protocols.
The Robetta server and revised automatic protocols were used to predict structures for CASP6 targets. Robetta is a publicly available protein structure prediction server (http://robetta.bakerlab.org/ that uses the Rosetta de novo and homology modeling structure prediction methods. We incorporated some of the lessons learned in the CASP5 experiment into the server prior to participating in CASP6. We additionally tested new ideas that were amenable to full-automation with an eye toward improving the server. We find that the Robetta server shows the greatest promise for the more challenging targets. The most significant finding from CASP5, that automated protocols can be roughly comparable in ability with the better human-intervention predictors, is repeated here in CASP6.
Chivian
Dylan
D
Department of Biochemistry, University of Washington, Seattle, Washington.
Kim
David E
DE
Department of Biochemistry, University of Washington, Seattle, Washington.
Malmström
Lars
L
Department of Biochemistry, University of Washington, Seattle, Washington.
Schonbrun
Jack
J
Howard Hughes Medical Institute, Seattle, Washington.
Rohl
Carol A
CA
Department of Biomolecular Engineering, University of California, Santa Cruz, California.
Baker
David
D
Department of Biochemistry, University of Washington, Seattle, Washington.|Howard Hughes Medical Institute, Seattle, Washington.
D000465:Algorithms; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D003201:Computers; D003627:Data Interpretation, Statistical; D030562:Databases, Protein; D019281:Dimerization; D008958:Models, Molecular; D009010:Monte Carlo Method; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D016415:Sequence Alignment; D012984:Software