title: Development of dense microsatellite markers in the entire SLA region and evaluation of their polymorphisms in porcine breeds.
abstract: We developed 40 microsatellite markers in the entire swine leukocyte antigen (SLA) region, spanning over 2.35 Mb. The average span between markers was 59 kb, and the largest interval between markers was 127 kb. We also evaluated polymorphisms of length for the markers using 97 pigs derived from 12 breeds, including representative commercial breeds. All of the markers were successfully amplified in genomic DNA and shown to be polymorphic. These markers will provide an alternative method for determining the SLA haplotypes instead of direct typing of SLA genes per se. They will be valuable for transplantation studies and for association studies between immunological traits such as disease susceptibility and tumor rejection.
authors.affiliation: Second Research Division, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki, 305-0854, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Tanaka
authors.forename: Asako
authors.initials: A
authors.lastname: Ando
authors.forename: Christine
authors.initials: C
authors.lastname: Renard
authors.forename: Patrick
authors.initials: P
authors.lastname: Chardon
authors.forename: Michiko
authors.initials: M
authors.lastname: Domukai
authors.forename: Naohiko
authors.initials: N
authors.lastname: Okumura
authors.forename: Takashi
authors.initials: T
authors.lastname: Awata
authors.forename: Hirohide
authors.initials: H
authors.lastname: Uenishi
mesh_terms: D000818:Animals; D001947:Breeding; D017931:DNA Primers; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D000949:Histocompatibility Antigens Class II; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D013552:Swine / Q000235:genetics*
chemical_list: D017931:DNA Primers; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen