title: Retrograde axonal transport of dopamine beta hydroxylase antibodies by neurons in the trigeminal ganglion.
abstract: In this study we describe a population of neurons in the adult rat trigeminal ganglion (TG) that express dopamine beta-hydroxylase (DBH) and tyrosine hydroxylase (TH), and transport anti-DBH from their terminals. We have used NGF and NT3 labeled with biotin and anti-p75NTR labeled with FITC to examine the transport of neurotrophins and their receptors by these cells. In both the superior cervical ganglion (SCG) and the TG all neurons that transported anti-DBH transported NGF. While 100% of the DBH positive neurons in the TG also transported NT3, approximately 25% of these neurons in the SCG failed to transport NT3. In the SCG virtually all the neurons transported anti-p75NTR with the neurotrophins while in the TG more than 25% of these neurons failed to transport anti-p75NTR with the neurotrophins. These findings suggest that DBH positive neurons in the TG depend upon target-derived NGF and NT3 for their noradrenergic phenotype.
authors.affiliation: The Scripps Research Institute, La Jolla, CA 90237, USA.
authors.forename: Anna J
authors.initials: AJ
authors.lastname: Reynolds
authors.forename: Selma K
authors.initials: SK
authors.lastname: Kaasinen
authors.forename: Ian A
authors.initials: IA
authors.lastname: Hendry
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001369:Axons; D001692:Biological Transport; D004299:Dopamine beta-Hydroxylase / Q000276:immunology*; D007150:Immunohistochemistry; D020932:Nerve Growth Factor / Q000378:metabolism; D009474:Neurons / Q000201:enzymology; D051381:Rats; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D000906:Antibodies; D020800:Receptor, Nerve Growth Factor; D020932:Nerve Growth Factor; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase