title: [Interaction between balofloxacin and DNA and the influence of Mg2+ on the interaction].
abstract: OBJECTIVE
  To study the binding mode of balofloxacin with DNA and evaluate the influence of Mg2+ on the binding between balofloxacin and DNA.
  
  
  METHODS
  Fluorescent spectroscopy was used to study the interaction of balofloxacin with DNA and to calculate the thermodynamic constants. UV-Vis spectra, DNA viscosity titration, competition experiment and the effect of dsDNA and ssDNA on the fluorescense intensity were used to identify the binding mode.
  
  
  RESULTS
  Balofloxacin interacted with CT-DNA with a quenching constant of (5.43 +/- 0.07) x 10(3) L x mol(-1). The interaction was exothermic with a Van't Hoff enthalply of - 8.03 kJ x mol(-1) x Mg2+ cation could enhance the quenching constant between balofloxacin and DNA.
  
  
  CONCLUSIONS
  Balofloxacin interacted with CT-DNA in the mode of groove binding and Mg2+ could mediate the binding of balofloxacin to DNA.
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210038, China. wenyingzhongnj@163.com
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Zhong
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Wang
authors.forename: Kun-Yi
authors.initials: KY
authors.lastname: Ni
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D024841:Fluoroquinolones / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D024841:Fluoroquinolones; D004247:DNA; D008274:Magnesium; C077155:balofloxacin