Feasibility on systemic delivery of asialoorosomucoid complex to hepatic origin cells mediated by asialoglycoprotein receptor.
Receptor mediated gene delivery is a new gene transfer strategy. Asialoglycoprotein receptor (ASGP-R), the receptor of asialoorosomucoid (Asor), is specially expressed on the surface of hepatocyte. In this paper, the nuclide 131I was combined with Asor to form a kind of soluble nuclide-protein complex, which can be specifically endocytosed into hepatocyte by ASGP-R. After in travenous injection of the complex into experimental animals, the deposition of Asor in vivo and the targeting quality of hepatocyte was detected by ECT. This research testified the feasibility of targeting Asor complex delivery to hepatocyte mediated by ASGP-R in vivo, and provided foundation for the genetic diagnosis and gene therapy of hepatic cell-related diseases.
Jun
Sun
S
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Xia
Jinsong
J
Wang
Yu
Y
Wang
Yuzhe
Y
Yiqiang
Zong
Z
Shen
Qu
Q
D000818:Animals; D037263:Asialoglycoprotein Receptor / Q000187:drug effects* / Q000502:physiology; D001212:Asialoglycoproteins / Q000378:metabolism*; D016503:Drug Delivery Systems / Q000379:methods; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology; D005240:Feasibility Studies; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009961:Orosomucoid / Q000031:analogs & derivatives* / Q000378:metabolism; D011485:Protein Binding; D011817:Rabbits; D014407:Tumor Cells, Cultured
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D007457:Iodine Radioisotopes; D009961:Orosomucoid; C017030:asialoorosomucoid