Inhibition of Anatid Herpes Virus-1 replication by small interfering RNAs in cell culture system.
RNA interference (RNAi) mediated by double stranded small interfering RNA (siRNA) is a novel mechanism of post-transcriptional gene silencing. It is projected as a potential tool to inhibit viral replication. In the present paper, we demonstrate the suppression of replication of an avian herpes virus (Anatid Herpes Virus-1, AHV-1) by siRNA mediated gene silencing in avian cells. The UL-6 gene of AHV-1 that codes for a protein involved in viral packaging was targeted. Both cocktail and unique siRNAs were attempted to evaluate the inhibitory potential of AHV-1 replication in duck embryo fibroblast (DEF) cell line. DEF cells were chemically transfected with different siRNAs in separate experiments followed by viral infection. The observed reduction in virus replication was evaluated by cytopathic effect, viral titration and quantitative real time PCR (QRT-PCR). Among the three siRNA targets used the unique siRNA UL-B sequence was found to be more potent in antiviral activity than the cocktail and UL6-A-siRNA sequences.
Mallanna
Sunil Kumar
SK
Biotechnology Laboratory, Indian Veterinary Research Institute, Mukteswar 263138, Nainital, Uttaranchal, India.
Rasool
T J
TJ
Sahay
Bikash
B
Aleyas
Abi George
AG
Ram
Hira
H
Mondal
Bimalendu
B
Nautiyal
Binita
B
Premraj
Avinash
A
Sreekumar
E
E
Yadav
M P
MP
D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis; D004372:Ducks; D006564:Herpesviridae / Q000235:genetics / Q000502:physiology*; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly; D014779:Virus Replication
D036022:Capsid Proteins; D004279:DNA, Viral; D034741:RNA, Small Interfering