title: Differential polyadenylation of ribosomal RNA during post-transcriptional processing in Leishmania.
abstract: The protozoan parasite Leishmania belongs to the most ancient eukaryotic lineages and this is reflected in several distinctive biological features, such as eukaryotic polycistronic transcription and RNA trans-splicing. The disclosure of this organism's unusual characteristics leads to a better understanding of the origin and nature of fundamental biological processes in eukaryotes. Here we report another unusual phenomenon as we demonstrate that precursor ribosomal RNA can be extensively polyadenylated during post-transcriptional processingt. Furthermore, we demonstrate that the degree of precursor rRNA polyadenylation is variable in different strains and in the different life-stages of a strain.
authors.affiliation: Department of Parasitology, Unit of Molecular Parasitology, Prince Leopold Institute of Tropical Medicine, Antwerp B-2000, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Decuypere
authors.forename: J
authors.initials: J
authors.lastname: Vandesompele
authors.forename: V
authors.initials: V
authors.lastname: Yardley
authors.forename: S
authors.initials: S
authors.lastname: De Donckeri
authors.forename: T
authors.initials: T
authors.lastname: Laurent
authors.forename: S
authors.initials: S
authors.lastname: Rijal
authors.forename: A
authors.initials: A
authors.lastname: Llanos-Cuentas
authors.forename: F
authors.initials: F
authors.lastname: Chappuis
authors.forename: J
authors.initials: J
authors.lastname: Arevalo
authors.forename: J C
authors.initials: JC
authors.lastname: Dujardin
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D007891:Leishmania / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D026723:Polyadenylation / Q000502:physiology*; D015800:Protozoan Proteins / Q000096:biosynthesis; D016053:RNA, Protozoan / Q000737:chemistry / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D020413:3' Untranslated Regions; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D012335:RNA, Ribosomal