title: Comparison of fundus autofluorescence and minimum-motion measurements of macular pigment distribution profiles derived from identical retinal areas.
abstract: The optical density of macular pigment was measured at twelve retinal locations in ten subjects by minimum motion photometry, comparing 460 nm with 580 nm or 550 nm. Fundus autofluorescence images were obtained for the same subjects with a scanning laser ophthalmoscope. Optical density was computed from mean calibrated grey-scale values for a central circular field and for annular segments, identical to areas tested psychophysically, and for complete annuli. Psychophysical assessments of optical density were similar irrespective of whether 550 nm or 580 nm was used. Optical density values derived psychophysically showed a linear correlation with assessments based on identical sampled areas of annular segments (slope = 0.98, r2 = 0.97) or complete annuli (slope = 0.89, r2 = 0.96) in autofluorescence images.
authors.affiliation: Moorfields Eye Hospital, London, UK. anthony.robson@moorfields.nhs.uk
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Robson
authors.forename: Glen
authors.initials: G
authors.lastname: Harding
authors.forename: Frederik J G M
authors.initials: FJ
authors.lastname: van Kuijk
authors.forename: Daniel
authors.initials: D
authors.lastname: Pauleikhoff
authors.forename: Graham E
authors.initials: GE
authors.lastname: Holder
authors.forename: Alan C
authors.initials: AC
authors.lastname: Bird
authors.forename: Frederick W
authors.initials: FW
authors.lastname: Fitzke
authors.forename: Jack D
authors.initials: JD
authors.lastname: Moreland
mesh_terms: D005451:Fluorescein Angiography; D006801:Humans; D008268:Macular Degeneration; D009039:Motion Perception / Q000502:physiology*; D010783:Photometry; D011601:Psychophysics; D012160:Retina / Q000502:physiology; D012168:Retinal Pigments / Q000502:physiology*
chemical_list: D012168:Retinal Pigments