High performance liquid chromatographic determination of platinum in blood and urine samples of cancer patients after administration of cisplatin drug using solvent extraction and N,N'-bis(salicylidene)-1,2-propanediamine as complexation reagent.
A high performance liquid chromatographic (HPLC) procedure has been developed for the determination of cisplatin, based on the pre-column derivation of platinum(II) with reagent N,N'-bis(salicylidene)-1,2-propanediamine (H2SA2pn). The neutral platinum complex was extracted, concentrated in an organic solvent and then injected (5 microl) on a reverse phase HPLC column, Varian Micro-Pak SP C-18, 5 microm (150 mm x 4.0 mm i.d.). The complex was eluted isocratically using a ternary mixture of methanol/acetonitrile/water (40/30/30, v/v/v) at a flow rate of 1.0 ml/min and was determined by a UV detector set at 254 nm after elution. A detection limit was found to be 4.0 ng per injection. The amounts of platinum in blood serum and urine of cancer patients after administration of cisplatin were observed in a range of 221-298 ng/ml and 43-97 ng/ml with relative standard deviation (R.S.D.) of 3.6-4.6% and 3.5-4.8%, respectively. Preliminary metabolism profiles of Pt concentrations in blood and urine from the patients were established.
Lanjwani
Shah Nawaz
SN
Department of Chemistry, the University of Western Ontario, London, Ont., Canada N6A 5B7.
Zhu
Renkang
R
Khuhawar
Mohammad Yar
MY
Ding
Zhifeng
Z
D000970:Antineoplastic Agents / Q000097:blood / Q000627:therapeutic use / Q000652:urine; D002614:Chelating Agents; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002945:Cisplatin / Q000097:blood / Q000627:therapeutic use / Q000652:urine; D003959:Diamines; D006801:Humans; D007202:Indicators and Reagents; D009369:Neoplasms / Q000097:blood* / Q000188:drug therapy / Q000652:urine*; D010984:Platinum / Q000097:blood* / Q000652:urine*; D012545:Schiff Bases; D013056:Spectrophotometry, Ultraviolet
D000970:Antineoplastic Agents; D002614:Chelating Agents; D003959:Diamines; D007202:Indicators and Reagents; C519577:N,N'-bis(salicylidene)-1.2-propanediamine; D012545:Schiff Bases; D010984:Platinum; D002945:Cisplatin