Study on the application of parent-of-origin specific DNA methylation markers to forensic genetics.
In paternity test, especially in motherless cases, the allele inherited from father (obligatory gene, OG) often cannot be determined. The paternity exclusion probability (PE) of a genetic marker is reduced considerably. Therefore, it is necessary to develop a new technique, by which the parental origin of alleles can be determined without genealogical analysis. In this paper, we explored the possibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles, choosing the imprinted single nucleotide polymorphism (SNP) locus rs220028 (A/G) as a model system. We typed the SNP by mutagenically separated PCR (MS-PCR). The frequencies of alleles were A = 0.5085, G = 0.4915; the unbiased heterozygosity was 0.5020. In order to discriminate between the maternal allele and paternal allele, post-digestion MS-PCR, a novel PCR based methylation analysis and SNP typing technique was developed and performed on 18 heterozygous children, and the methylated maternal allele was detected specifically. As a pilot study on the use of epigenetic markers in forensic genetics, our results demonstrated the feasibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles.
Zhao
Guisen
G
Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China. shenxiaomo2003@yahoo.com
Yang
Qingen
Q
Huang
Daixin
D
Yu
Chunying
C
Yang
Rongzhi
R
Chen
Hui
H
Mei
Kun
K
D000483:Alleles; D002648:Child; D016172:DNA Fingerprinting / Q000379:methods*; D019175:DNA Methylation; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide
D005819:Genetic Markers