Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas.
The complex interactions between cancer and host cells are far from being fully elucidated. Assessment of Th1/Th2/Th3/Tr1 balance is an interesting approach to explain immunological disturbances in lymphomas. The aim of our study was to assess mRNA for pro- and anti-inflammatory cytokines in T-cells in 20 children with Hodgkin- and non-Hodgkin lymphomas. CD4+ and CD8+ cells were isolated from whole peripheral blood and four different cytokine mRNA levels (IFN-gamma, IL-10, IL-4, TGF-beta) were determined by real-time PCR technique. Comparing to the control group, we found lower expression of mRNA for IFN-gamma in CD4+ cells at the time of lymphoma diagnosis. It may be one of the pathogenetic mechanisms of impaired immunity in these patients.
Luczyński
Włodzimierz
W
Department of Pediatric Hematology and Oncology, Medical University, Bialystok, Poland. vlodek@amb.edu.pl
Kovalchuk
Oksana
O
Krawczuk-Rybak
Maryna
M
Mitura-Lesiuk
Małgorzata
M
Malinowska
Iwona
I
Chyczewski
Lech
L
Kowalczyk
Jerzy
J
Matysiak
Michał
M
D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005786:Gene Expression Regulation; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008223:Lymphoma / Q000175:diagnosis* / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
D012333:RNA, Messenger; D007371:Interferon-gamma