Polymorphism in Alnus based Frankia of Darjeeling.
DNA samples extracted from the root nodules of Alnus nepalensis, collected from 10 different locations of Darjeeling hills, were used to assess the genetic diversity of Frankia. The DNA samples from the nodules of naturally growing plants were used as templates in PCR, targeting different genomic regions of Frankia, namely distal, middle and proximal parts of 16S rRNA gene and nifH-D IGS region with locus specific primers. The PCR products were digested with a number of frequent (4-base) cutter restriction endonucleases. Bands were scored as present (1) or absent (0) and the clustering was done using NTSYSpc. Distinct polymorphism was found among the nodules collected from different parts of the region and those of same geographic area. These results demonstrate that genetic diversity is indeed present among the naturally occurring Frankia of Darjeeling, India.
Bajwa
Manprit
M
Molecular Genetics Laboratory, Department of Botany, University of North Bengal, Siliguri, 734 430, India.
Sen
Arnab
A
Bajwa
Balwinder Singh
BS
D029661:Alnus / Q000235:genetics*; D016000:Cluster Analysis; D017931:DNA Primers / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D040161:Frankia / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S