Different neuronal populations of the rat median preoptic nucleus express c-fos during sleep and in response to hypertonic saline or angiotensin-II.
The median preoptic nucleus (MnPN) of the hypothalamus contains sleep-active neurones, and sleep-related Fos-immunoreactivity (IR) in this nucleus is primarily expressed in GABAergic cells. The MnPN also contains cells responsive to hypertonic saline and to angiotensin-II (Ang-II). To clarify functional relationships between MnPN neurones involved in the regulation of sleep and body fluid homeostasis, we examined c-fos expression in the MnPN after administration of hypertonic saline and Ang-II in both spontaneously sleeping and sleep-deprived rats. Systemic administration of hypertonic saline and intracerebroventricular (i.c.v.) injection of Ang-II increased Fos-IR in both spontaneously sleeping and sleep-deprived rats, compared to control animals. To determine if the population of MnPN neurones activated in response to osmotic and hormonal stimuli is similar to or different from neurones activated during sleep, we quantified Fos-IR in MnPN GABAergic neurones in spontaneously sleeping hypertonic saline- and Ang-II-treated rats versus respective control rats. Fos-IR evoked by these treatments occurred primarily (80-85%) in non-GABAergic neurones. Findings of the present study provide evidence that separate populations of MnPN neurones are involved in the regulation of sleep and body fluid homeostasis.
Gvilia
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I
Research Service (151A3), V.A. Greater Los Angeles Healthcare System, 16111 Plummer Street, North Hills, CA 91344, USA.
Angara
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C
McGinty
D
D
Szymusiak
R
R
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001826:Body Fluids / Q000502:physiology; D002452:Cell Count; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006706:Homeostasis / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D011301:Preoptic Area / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis* / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D012890:Sleep / Q000502:physiology*; D012892:Sleep Deprivation / Q000503:physiopathology; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D016760:Proto-Oncogene Proteins c-fos; D012462:Saline Solution, Hypertonic; D000804:Angiotensin II; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase