title: Bacteriological study of autologous cryoprecipitate-derived fibrin glue as the operative sealant.
abstract: Fibrin glue (FG) is frequently used to seal and cover the anastomoses in many operations such as cardiovascular surgery or orthopaedic surgery. However, in case of gastrointestinal surgery, anastomoses are potentially contaminated, and FG may promote bacterial growth, increasing the risk of leakage. The purpose of this study was to examine the effect of cryoprecipitate-derived FG (CryoFG) on bacterial growth. Bacterial growth on the CryoFG and on the commercial FG (Beriplast P) was evaluated and compared with that on control medium. In addition, the complement activities were evaluated by heat inactivation or addition of guinea-pig complement to the experimental settings. The CryoFG inhibited the growth of Escherichia coli, whereas the commercial FG had no effect. Heat inactivation of the CryoFG inhibited the bactericidal effect of CryoFG. Addition of guinea-pig complement to the heat-inactivated CryoFG could almost restore the bactericidal activity, suggesting the important role of complement. This study showed that the CryoFG preserved the complement activity, which inhibited the in vitro growth of E. coli. Therefore, we concluded that the application of the CryoFG for gastrointestinal surgical anastomoses not only would be safe but also has the advantage of reducing bacterial infection.
authors.affiliation: Department of Surgery, Toranomon Hospital, Tokyo, Japan. yoshihirokinoshita@nifty.com
authors.forename: Y
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: H
authors.initials: H
authors.lastname: Udagawa
authors.forename: K
authors.initials: K
authors.lastname: Tsutsumi
authors.forename: M
authors.initials: M
authors.lastname: Ueno
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Iizuka
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
authors.forename: M
authors.initials: M
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Sawada
authors.forename: G
authors.initials: G
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Tsuno
authors.forename: A
authors.initials: A
authors.lastname: Wada
authors.forename: H
authors.initials: H
authors.lastname: Inagawa
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D013505:Digestive System Surgical Procedures / Q000379:methods; D005169:Factor VIII / Q000494:pharmacology*; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D005340:Fibrinogen / Q000494:pharmacology*; D006801:Humans; D014014:Tissue Adhesives / Q000494:pharmacology*; D014182:Transplantation, Autologous
chemical_list: D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; C026912:cryoprecipitate coagulum; D005169:Factor VIII; D005340:Fibrinogen