title: Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: general implications and practical use in endodontic antimicrobial therapy.
abstract: By re-examining 10 previously published "universal" PCR assays using the ARB phylogenetic software package and database with 41,000 16S rRNA gene sequences, we found that they differed considerably in their coverage of the domain Bacteria. We evaluated the broadest-range real-time quantitative PCR protocol for its efficacy in measuring the antimicrobial effects of endodontic treatments.
authors.affiliation: Division of Oral Microbiology and Immunology, Department of Operative and Preventive Dentistry & Peridontology, RWTH Aachen University Hospital, Aachen, Germany.
authors.forename: H P
authors.initials: HP
authors.lastname: Horz
authors.forename: M E
authors.initials: ME
authors.lastname: Vianna
authors.forename: B P F A
authors.initials: BP
authors.lastname: Gomes
authors.forename: G
authors.initials: G
authors.lastname: Conrads
mesh_terms: D000328:Adult; D000368:Aged; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000627:therapeutic use*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000188:drug therapy / Q000382:microbiology; D002710:Chlorhexidine / Q000008:administration & dosage / Q000627:therapeutic use*; D015342:DNA Probes; D003786:Dental Pulp Cavity / Q000382:microbiology; D004708:Endodontics / Q000379:methods; D006801:Humans; D008875:Middle Aged; D010483:Periapical Diseases / Q000188:drug therapy / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012973:Sodium Hypochlorite / Q000008:administration & dosage / Q000627:therapeutic use*; D012984:Software
chemical_list: D000891:Anti-Infective Agents, Local; D015342:DNA Probes; D012336:RNA, Ribosomal, 16S; D012973:Sodium Hypochlorite; D002710:Chlorhexidine