Sensitive LC MS quantitative analysis of carbohydrates by Cs+ attachment.
The development of a sensitive assay for the quantitative analysis of carbohydrates from human plasma using LC/MS/MS is described in this paper. After sample preparation, carbohydrates were cationized by Cs(+) after their separation by normal phase liquid chromatography on an amino based column. Cesium is capable of forming a quasi-molecular ion [M + Cs](+) with neutral carbohydrate molecules in the positive ion mode of electrospray ionization mass spectrometry. The mass spectrometer was operated in multiple reaction monitoring mode, and transitions [M + 133] --> 133 were monitored (M, carbohydrate molecular weight). The new method is robust, highly sensitive, rapid, and does not require postcolumn addition or derivatization. It is useful in clinical research for measurement of carbohydrate molecules by isotope dilution assay.
Rogatsky
Eduard
E
General Clinical Research Center, Albert Einstein College of Medicine, Golding Building Rm. G02, 1300 Morris Park Avenue, Bronx, NY 10461, USA. rogatsky@aecom.yu.edu
Jayatillake
Harsha
H
Goswami
Gayotri
G
Tomuta
Vlad
V
Stein
Daniel
D
D001774:Blood Chemical Analysis / Q000379:methods*; D002241:Carbohydrates / Q000097:blood* / Q000737:chemistry*; D002586:Cesium / Q000097:blood* / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008832:Microchemistry / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013194:Staining and Labeling
D002241:Carbohydrates; D002586:Cesium