Mie theory interpretations of light scattering from intact cells.
We present a Mie theory-based analysis of angularly resolved light scattering from intact cells that involves the product of a particle size distribution rho and the scattering cross section sigma. The analysis shows that the ability to size intracellular organelles depends strongly upon the assumed functional form of the particle size distribution; however, the product sigma rho extracts the dominant light scatterers regardless of this choice. We examine goniometer measurements from our own and another laboratory and find that this analysis reconciles seemingly conflicting results and confirms that mitochondrial-sized organelles dominate scattering from intact cells in the angular range 50 degrees - 90 degrees.
Wilson
Jeremy D
JD
Department of Radiology, University of Rochester, 601 Elmwood Avenue, Box 648, Rochester, New York 14642, USA. jdwilson@pas.rochester.edu
Foster
Thomas H
TH
D000465:Algorithms; D000818:Animals; D045744:Cell Line, Tumor; D003198:Computer Simulation; D008027:Light; D008325:Mammary Neoplasms, Experimental / Q000473:pathology* / Q000503:physiopathology*; D051379:Mice; D008954:Models, Biological; D010316:Particle Size; D010783:Photometry / Q000379:methods*; D011829:Radiation Dosage; D011874:Radiometry; D012031:Refractometry / Q000379:methods*; D012542:Scattering, Radiation