Application of an electric DNA-chip for the expression analysis of bioprocess-relevant marker genes of Bacillus subtilis.
The knowledge of critical process-relevant genes can be used for an improved control of bioprocesses. So far bioprocess-relevant marker genes can be analyzed by established expression analysis methods only off-line. In this study, an alternative approach for a potential at-line monitoring of gene expression during bioprocesses is suggested. This approach is based on the measurement of specific mRNAs on an electric DNA-chip in connection with a magnetic bead-based sandwich hybridization. In order to allow an at-line measurement of specific mRNAs an improved method for a fast and partially automated isolation of high quality-RNA samples was developed. The expression analysis of the electric DNA-chip was compared with optical DNA micro arrays and the real time RT-PCR for three selected process-relevant genes of Bacillus subtilis. We demonstrate that the mRNA analysis by means of the electric DNA-chip gives similar results compared to the micro array analysis and the real time RT-PCR technique.
Jürgen
Britta
B
Institute of Microbiology, Ernst-Moritz-Arndt-University Greifswald, F.-L.-Jahnstrasse 15, D-17487 Greifswald, Germany.
Barken
Kim Bundvig
KB
Tobisch
Steffen
S
Pioch
Daniel
D
Wümpelmann
Mogens
M
Hecker
Michael
M
Schweder
Thomas
T
D001412:Bacillus subtilis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D015415:Biomarkers; D004563:Electrochemistry / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009862:Online Systems
D001426:Bacterial Proteins; D015415:Biomarkers