Biodegradation of lignocellulosics: microbial, chemical, and enzymatic aspects of the fungal attack of lignin.
Wood is the main renewable material on Earth and is largely used as building material and in paper-pulp manufacturing. This review describes the composition of lignocellulosic materials, the different processes by which fungi are able to alter wood, including decay patterns caused by white, brown, and soft-rot fungi, and fungal staining of wood. The chemical, enzymatic, and molecular aspects of the fungal attack of lignin, which represents the key step in wood decay, are also discussed. Modern analytical techniques to investigate fungal degradation and modification of the lignin polymer are reviewed, as are the different oxidative enzymes (oxidoreductases) involved in lignin degradation. These include laccases, high redox potential ligninolytic peroxidases (lignin peroxidase, manganese peroxidase, and versatile peroxidase), and oxidases. Special emphasis is given to the reactions catalyzed, their synergistic action on lignin, and the structural bases for their unique catalytic properties. Broadening our knowledge of lignocellulose biodegradation processes should contribute to better control of wood-decaying fungi, as well as to the development of new biocatalysts of industrial interest based on these organisms and their enzymes.
Martínez
Angel T
AT
Biological Research Center, CSIC, Madrid, Spain. ATMartinez@cib.csic.es
Speranza
Mariela
M
Ruiz-Dueñas
Francisco J
FJ
Ferreira
Patricia
P
Camarero
Susana
S
Guillén
Francisco
F
Martínez
María J
MJ
Gutiérrez
Ana
A
del Río
José C
JC
D001673:Biodegradation, Environmental; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology / Q000378:metabolism*; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism; D014934:Wood
C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D010088:Oxidoreductases; D010544:Peroxidases