Macrolide resistance mediated by a Bifidobacterium breve membrane protein.
A gene coding for a hypothetical membrane protein from Bifidobacterium breve was expressed in Lactococcus lactis. Immunoblotting demonstrated that this protein is located in the membrane. Phenotypical changes in sensitivity towards 21 antibiotics were determined. The membrane protein-expressing cells showed higher levels of resistance to several macrolides.
Margolles
Abelardo
A
Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (CSIC), Ctra. Infiesto s/n, Villaviciosa, Spain. amargolles@ipla.csic.es
Moreno
José Antonio
JA
van Sinderen
Douwe
D
de Los Reyes-Gavilán
Clara G
CG
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001644:Bifidobacterium / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D013294:Lactococcus lactis / Q000235:genetics; D018942:Macrolides / Q000494:pharmacology*; D008565:Membrane Proteins / Q000378:metabolism*; D008826:Microbial Sensitivity Tests
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; D008565:Membrane Proteins