title: Comparative immune responses in Japanese flounder, Paralichthys olivaceus after vaccination with viral hemorrhagic septicemia virus (VHSV) recombinant glycoprotein and DNA vaccine using a microarray analysis.
abstract: A viral hemorrhagic septicemia virus (VHSV), recombinant glycoprotein vaccine (VHSVg) and a DNA vaccine (pCMV-VHSg) were injected in Japanese flounder (Paralichthys olivaceus). Each fish was injected with 10 microg vaccine dissolved in 50 microl phosphate buffer saline (PBS). One month after the vaccination, the fish were challenged intraperitoneally with either 1 x 10(2) or 1 x 10(3) TCID(50) of virus. Fish that received the VHSg DNA vaccine were highly protected against virus infection over a 21-day observation period, with cumulative mortalities ranging from 4 to 10%. However, the recombinant protein vaccine group appeared to have low survival rates. Using microarray analysis, humoral defense-related genes such as complement component C3, complement regulatory plasma proteins, IgM, IgD, MHC class II-associated invariant chain and CD20 receptor were observed to be up-regulated by the VHSg recombinant protein vaccine at 1 or 21 days post vaccination. On the other hand, cellular defense-related genes such as CD8 alpha chain, T-cell immune regulator, MIP1-alpha and apoptosis-associated protein were not detected. Specific antibodies against VHSVg protein were detected in both vaccinated groups at a titer of 1:40 at 28 days post vaccination.
authors.affiliation: Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Japan.
authors.forename: Ju Yong
authors.initials: JY
authors.lastname: Byon
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ohira
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D005432:Flounder / Q000821:virology*; D020869:Gene Expression Profiling; D031941:Hemorrhagic Septicemia, Viral / Q000517:prevention & control*; D029141:Novirhabdovirus / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D014611:Vaccination / Q000662:veterinary*; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D014765:Viral Vaccines