Fluorescence resonance energy transfer between a quantum dot donor and a dye acceptor attached to DNA.
We show that direct coupling of a dye-labelled DNA (acceptor) to a quantum dot (QD) donor significantly reduces the donor-acceptor distance and improves the FRET efficiency: a highly efficient FRET (approximately 88%) at a low acceptor-to-donor ratio of 2 has been achieved at the single-molecule level.
Zhou
Dejian
D
Nanoscience Centre, University of Cambridge, UK. dz209@cam.ac.uk
Piper
Joe D
JD
Abell
Chris
C
Klenerman
David
D
Kang
Dae-Joon
DJ
Ying
Liming
L
D019187:Cadmium Compounds / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D013997:Time Factors; D017967:Zinc Compounds / Q000737:chemistry
D019187:Cadmium Compounds; D004396:Coloring Agents; D018036:Selenium Compounds; D013438:Sulfhydryl Compounds; D013440:Sulfides; D017967:Zinc Compounds; D004247:DNA; C058667:cadmium selenide; C031238:zinc sulfide