T-DNA transfer from Agrobacterium tumefaciens to the ectomycorrhizal fungus Pisolithus microcarpus.
The model ectomycorrhizal fungus Pisolithus microcarpus isolate 441 was transformed by using Agrobacterium tumefaciens LBA1100 and AGL-1. The selection marker was the Shble gene of Streptoallotecius hidustanus, conferring resistance to phleomycin, under the control of the gpd gene promoter and terminator of Schizophyllum commune. Transformation resulted in phleomycin resistant clones which were confirmed by PCR to contain the resistance cassette. A. tumefaciens-mediated gene transfer would allow the development of RNA interference technology in P. microcarpus.
Pardo
A G
AG
Programa de Investigación en Interacciones Biológicas, Universidad Nacional de Quilmes, Roque Sáenz Peña 180, (B1876BXD) Bernal, Provincia de Buenos Aires, Argentina. apardo@unq.edu.ar
Kemppainen
M
M
Valdemoros
D
D
Duplessis
S
S
Martin
F
F
Tagu
D
D
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics*; D001487:Basidiomycota / Q000187:drug effects / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D025141:Drug Resistance, Fungal / Q000235:genetics; D005813:Genes, Synthetic; D010692:Phleomycins / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D012567:Schizophyllum / Q000235:genetics; D012641:Selection, Genetic; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010692:Phleomycins; C036483:T-DNA; C099353:phleomycin resistance protein, Streptoalloteichus hindustanus