title: Mechanisms of MHC class I-restricted antigen processing and cross-presentation.
abstract: In this review, we discuss recent data from our laboratory that address two aspects of major histocompatibility complex (MHC) class I-restricted antigen processing. First, we consider the nature of the peptide-loading complex, which is the assembly of proteins in the endoplasmic reticulum (ER) into which newly synthesized MHC class I-beta(2) microglobulin (beta(2)m) heterodimers are incorporated, and the mechanisms involved in MHC class I assembly and peptide loading that are facilitated by the peptide-loading complex. Second, we discuss mechanisms of cross-presentation, the phenomenon whereby extracellular and luminal protein antigens can be processed by antigen-presenting cells, particularly dendritic cells, and presented by MHC class I molecules to CD8(+) T cells. The focus of the discussion is mainly on the human MHC class I system.
authors.affiliation: Section of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520, USA. peter.cresswell@yale.edu
authors.forename: Peter
authors.initials: P
authors.lastname: Cresswell
authors.forename: Anne L
authors.initials: AL
authors.lastname: Ackerman
authors.forename: Alessandra
authors.initials: A
authors.lastname: Giodini
authors.forename: David R
authors.initials: DR
authors.lastname: Peaper
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Wearsch
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D045142:Cross-Priming / Q000276:immunology*; D004721:Endoplasmic Reticulum / Q000276:immunology / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D018448:Models, Immunological; D010455:Peptides / Q000276:immunology / Q000378:metabolism
chemical_list: D015395:Histocompatibility Antigens Class I; D010455:Peptides