title: Culture-independent analysis of fecal microbiota in cattle.
abstract: The phylogenetic diversity of the fecal bacterial community in Holstein cattle was determined by 16S ribosomal RNA gene sequence analysis. The sequences were affiliated with the following phyla: Firmicutes (81.3%), Bacteroidetes (14.4%), Actinobacteria (2.5%), and Proteobacteria (1.4%). The Clostridium leptum subgroup was the most phylogenetically diverse group in cattle feces. In addition, a number of previously uncharacterized and unidentified bacteria were recognized in clone libraries.
authors.affiliation: United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Ozutsumi
authors.forename: Hidenori
authors.initials: H
authors.lastname: Hayashi
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Hisao
authors.initials: H
authors.lastname: Itabashi
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Benno
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification*; D002417:Cattle; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA; D010802:Phylogeny; D017421:Sequence Analysis / Q000379:methods*