title: A novel method for immediate post-purification purity determination of fractions collected during high-throughput purification.
abstract: Following purification, the fractions of purified samples typically are analyzed to determine the relative purities of each fraction. We report a novel technique for performing post-purification analysis immediately after each preparative LC/MS run. The Single Pass Compound Purification and Analysis System (SPACPASS) samples and stores a representative aliquot from the fraction while it is being collected. Demonstrated for '1:1' fraction collections, this method of fraction purity assessment streamlined sample processing by reducing post-purification sample handling. For 97% of the collected fractions, this technique provided relative purities to within +/-5% when compared with more traditional post-purification analysis.
authors.affiliation: Department of Analytical and Bioanalytical Chemistry, Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA.
authors.forename: Ding
authors.initials: D
authors.lastname: Yuan
authors.forename: Chris
authors.initials: C
authors.lastname: Petersen
authors.forename: Matthew
authors.initials: M
authors.lastname: Rynd
authors.forename: James
authors.initials: J
authors.lastname: Mainquist
authors.forename: Andrew
authors.initials: A
authors.lastname: Meyer
authors.forename: John J
authors.initials: JJ
authors.lastname: Isbell
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet