title: Cabut, a C2H2 zinc finger transcription factor, is required during Drosophila dorsal closure downstream of JNK signaling.
abstract: During dorsal closure, the lateral epithelia on each side of the embryo migrate dorsally over the amnioserosa and fuse at the dorsal midline. Detailed genetic studies have revealed that many molecules are involved in this epithelial sheet movement, either with a signaling function or as structural or motor components of the process. Here, we report the characterization of cabut (cbt), a new Drosophila gene involved in dorsal closure. cbt is expressed in the yolk sac nuclei and in the lateral epidermis. The Cbt protein contains three C2H2-type zinc fingers and a serine-rich domain, suggesting that it functions as a transcription factor. cbt mutants die as embryos with dorsal closure defects. Such embryos show defects in the elongation of the dorsal-most epidermal cells as well as in the actomyosin cable assembly at the leading edge. A combination of molecular and genetic analyses demonstrates that cbt expression is dependent on the JNK cascade during dorsal closure, and it functions downstream of Jun regulating dpp expression in the leading edge cells.
authors.affiliation: Departamento de Genética, Facultad CC Biológicas, University of Valencia, Dr. Moliner 50, 46100 Burjasot, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Muñoz-Descalzo
authors.forename: Javier
authors.initials: J
authors.lastname: Terol
authors.forename: Nuria
authors.initials: N
authors.lastname: Paricio
mesh_terms: D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004331:Drosophila melanogaster / Q000196:embryology* / Q000201:enzymology / Q000235:genetics; D000078404:Epidermal Cells; D048031:JNK Mitogen-Activated Protein Kinases / Q000502:physiology*; D009154:Mutation; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D016335:Zinc Fingers / Q000235:genetics
chemical_list: C506602:Cbt protein, Drosophila; D029721:Drosophila Proteins; D014157:Transcription Factors; C064196:dpp protein, Drosophila; D048031:JNK Mitogen-Activated Protein Kinases