Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates.
Nuclear envelope (NE) formation during cell division in multicellular organisms is a central yet poorly understood biological process. We report that the conserved nucleoporin Nup155 has an essential function in NE formation in Caenorhabditis elegans embryos and in Xenopus laevis egg extracts. In vivo depletion of Nup155 led to failure of nuclear lamina formation and defects in chromosome segregation at anaphase. Nup155 depletion inhibited accumulation of nucleoporins at the nuclear periphery, including those recruited to chromatin early in NE formation. Electron microscopy analysis revealed that Nup155 is also required for the formation of a continuous nuclear membrane in vivo and in vitro. Time-course experiments indicated that Nup155 is recruited to chromatin at the time of NE sealing, suggesting that nuclear pore complex assembly has to progress to a relatively late stage before NE membrane assembly occurs.
Franz
Cerstin
C
European Molecular Biology Laboratory, Heidelberg, Germany.
Askjaer
Peter
P
Antonin
Wolfram
W
Iglesias
Carmen López
CL
Haselmann
Uta
U
Schelder
Malgorzata
M
de Marco
Ario
A
Wilm
Matthias
M
Antony
Claude
C
Mattaj
Iain W
IW
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029742:Caenorhabditis elegans Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D020090:Chromosome Segregation / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism; D009685:Nuclear Envelope / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D034881:Nuclear Lamina / Q000235:genetics / Q000378:metabolism; D022022:Nuclear Pore / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D029867:Xenopus Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000235:genetics / Q000378:metabolism*
D029742:Caenorhabditis elegans Proteins; D002843:Chromatin; C506179:NUP155 protein, Xenopus; D028861:Nuclear Pore Complex Proteins; D029867:Xenopus Proteins; C506178:npp-8 protein, C elegans