[Protective effect of human decay accelerating factor on mouse cells].
OBJECTIVE
To observe the protective effect of human decay accelerating factor (DAF) expressed in mouse myeloma cells against the injury of human serum complement.


METHODS
Mouse myeloma cells were transfected with human DAF cDNA and then put into normal human serum culture to observe if they were injured.


RESULTS
The mouse cells containing human DAF cDNA and expressing human DAF survived for more than 48 hours and passed on from generation to generation in the culture medium containing human normal serum. However, the control mouse cells died in 1 - 4 hours.


CONCLUSIONS
Human DAF protects mouse cells from injuring by human complement.
Zhao
Y
Y
Department of Urology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
Li
L
L
Fu
Y
Y
D000818:Animals; D018958:CD55 Antigens / Q000235:genetics / Q000494:pharmacology*; D003165:Complement System Proteins / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D051379:Mice; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology; D020011:Protective Agents / Q000494:pharmacology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D018958:CD55 Antigens; D018076:DNA, Complementary; D020011:Protective Agents; D003165:Complement System Proteins