[Analysis of VP7 serotype of rotavirus in infantile diarrhea in Hangzhou area from 2001 to 2003].
OBJECTIVE
To investigate molecular epidemiologic features of rotavirus (RV) infection in infantile diarrhea in Hangzhou area.


METHODS
Stool specimens of 683 infants with suspected acute viral enteritis in the autumn and winter of 2001 - 2003 were collected. RV (group A) was detected by using latex agglutination test (LAT). VP7 serotype (G) positive specimens were detected by using enzyme linked immunosorbent assay (ELISA) and then the RNA of the virus was determined with reverse transcription polymerase chain reaction (RT-PCR). cDNA of VP7 gene fragment was sequenced by automatic gene analyzor (ABI3730) and compared with the RV VP7 gene sequences stored in Genebank.


RESULTS
RV was detected in 297 of 683 (43.5%) specimens by LAT. The highest frequency of RV (group A) detected was 52.9% (228/431) in patients aged 7 - 18 months. The prevalent serotypes were G1 (36.7%, 109/297) and G3 (30.9%, 92/297), followed by mixed type (11.8%, 35/297), untyped (9.4%, 28/297), G4 (7.1%, 21/297) and G2 (4.0%, 12/297). The prevalent serotypes seen each year were different. G1 (54.9%, 45/82) was the major serotype in 2001 followed by G3 (14.6%, 12/82). In 2003, the major serotype was G3 (43.0%, 63/146) and followed by G1 (29.5%, 43/146). The reliability of ELISA was confirmed by RT-PCR, gene sequencing and homology analysis.


CONCLUSIONS
The main prevalent serotypes of VP7 of rotavirus were G1 and G3. The dominant serotypes of rotavirus varied in Hangzhou area from 2001 to 2003.
Tong
Mei-qin
MQ
Department of Gastroenterology, Children's Hospital Affiliated to The Medical College, Zhejiang University, Hangzhou 310003, China.
Chen
Li-qin
LQ
Shang
Shi-qiang
SQ
Zhao
Zheng-yan
ZY
D000956:Antigens, Viral / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D036022:Capsid Proteins / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003968:Diarrhea, Infantile / Q000453:epidemiology / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D007841:Latex Fixation Tests; D008297:Male; D008969:Molecular Sequence Data; D015995:Prevalence; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000150:complications / Q000453:epidemiology* / Q000821:virology*; D012703:Serotyping
D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus