title: Lignans from the roots of Wikstroemia indica and their DPPH radical scavenging and nitric oxide inhibitory activities.
abstract: In our investigation of in vitro anti-allergic screening of medicinal herbal extracts, the ethyl acetate extract of the root of Wikstroemia indica was observed to inhibit nitric oxide (NO) production in a lipopolysaccharide (LPS) and recombinant mouse interferon-gamma (IFN-gamma) activated murine macrophage-like cell line, RAW 264.7. Fractionation of the active extract led to the isolation of two new lignan dimer bis-5,5-nortrachelogenin (1) and bis-5,5'-nortrachelogenin (2), and three known lignans. Compound 1 and lirioresinol B (5) inhibited NO production with IC50 values at 48.6 and 53.5 microM, respectively. Compound 5 also showed free radical scavenging effect on 1,1-diphenyl-2-picrylhydrazil (DPPH) at IC50 values at 19.5 microM.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Nihon University, Funabashi, Chiba, Japan.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Wang
authors.forename: Noriko
authors.initials: N
authors.lastname: Unehara
authors.forename: Susumu
authors.initials: S
authors.lastname: Kitanaka
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds; D002247:Carbon Isotopes; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007371:Interferon-gamma / Q000037:antagonists & inhibitors; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy / Q000379:methods / Q000592:standards; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010851:Picrates / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011522:Protons; D011994:Recombinant Proteins / Q000187:drug effects; D012015:Reference Standards; D032365:Wikstroemia / Q000737:chemistry*
chemical_list: D001713:Biphenyl Compounds; D002247:Carbon Isotopes; D016166:Free Radical Scavengers; D017705:Lignans; D008070:Lipopolysaccharides; D010851:Picrates; D010936:Plant Extracts; D011522:Protons; D011994:Recombinant Proteins; D009569:Nitric Oxide; D007371:Interferon-gamma; C004931:1,1-diphenyl-2-picrylhydrazyl