[Research on resveratrol's effects on suppressing growth and inducing apoptosis of GBC cells].
OBJECTIVE
To explore the contribution of resveratrol (Res) to the proliferation and apoptosis of gallbladder carcinoma (GBC) cell line as well as fibroblast 3T3 cell line in vitro.


METHODS
The tumor cell growth repressing rate was measured by the MTT method,and flow cytometry was used to analyse cell cycle. The gene expression of bcl-2 c-myc p53 were examined by SABC.


RESULTS
Res obviously suppressed the proliferation (P <0. 01) in concentration and time dependent manner, it's tiptop was 54%. Res could induce apoptosis of tumor cells. The maximum apoptosis rate of tumor was 30. 52%, experiment group compared with comparison group Gl's cells rised from 34. 88% to 55.47 +/- 3. 95%, S's cells reduced 8.41 - 17.54%, which showed obvious phenomenon of G0/G1 blocking. The GBC cell's bcl-2, c-myc gene reduced expression,while p53 gene increased expression.


CONCLUSIONS
The results of this study confirm the ability of Res to suppress the proliferation of gallbladder carcinoma (GBC) cell with a typical apoptotic feature in vitro. Therefore, Res may be considered as a possible treatment strategy for gallbladder carcinoma.
Sheng
Li
L
Department of Immunology, Medical College, Xi'an Jiaotong University, Xi'an.
An
Lifeng
L
He
Ye
Y
Fan
Guixiang
G
Yuan
Yukang
Y
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D005434:Flow Cytometry; D005706:Gallbladder Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry*; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D013267:Stilbenes; D000077185:Resveratrol