title: Effect of glycoamphiphiles on the solubilization and dendritic cell uptake of a lipopeptide: a preliminary study.
abstract: The selective delivery of antigens to professional antigen-presenting cells represents a promising approach to improve vaccine efficacy. Addition of a glycoamphiphile to a lipopeptide, whose interest for vaccination is now well-established, greatly favors its solubilization in aqueous solutions through the formation of mixed vesicles. Flow cytometry experiments indicate that this formulation does not diminish the uptake of the lipopeptide by the dendritic cells (DCs). These preliminary results suggest a possible straightforward, noncovalent targeting of cocktail-lipopeptide vaccines to the DCs via carbohydrate receptor-mediated endocytosis.
authors.affiliation: SEDAC-Therapeutics, Parc Eurasanté, Le Galénis, 85 rue Nelson Mandela, 59120 Loos, France. dominique.bonnet@pharma.u-srasbg.fr
authors.forename: Dominique
authors.initials: D
authors.lastname: Bonnet
authors.forename: Gerhild
authors.initials: G
authors.lastname: Angyalosi
authors.forename: Alexandra
authors.initials: A
authors.lastname: Demory
authors.forename: Valérie
authors.initials: V
authors.lastname: Santraine
authors.forename: Arnaud
authors.initials: A
authors.lastname: Boulet
authors.forename: Corentin
authors.initials: C
authors.lastname: Spriet
authors.forename: Laurent
authors.initials: L
authors.lastname: Héliot
authors.forename: Hélène
authors.initials: H
authors.lastname: Gras-Masse
authors.forename: Cyrille
authors.initials: C
authors.lastname: Grandjean
mesh_terms: D001692:Biological Transport; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism*; D004705:Endocytosis; D005434:Flow Cytometry; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008074:Lipoproteins / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D012235:Rhodamines; D012995:Solubility; D014612:Vaccines
chemical_list: D008074:Lipoproteins; D010455:Peptides; D012235:Rhodamines; D014612:Vaccines; D005947:Glucose