Genetic vaccination for production of DNA-designed antibodies specific to Hepadnavirus envelope proteins.
We propose a method of avian antibodies production based on DNA immunization of laying ducks with a plasmid encoding specified antigen, followed by egg collection and purification of egg yolk immunoglobulins (IgY). We have validated this approach in the Duck hepatitis B virus (DHBV) model. We report here that following immunization of female ducks with plasmids encoding DHBV envelope proteins, large amounts (at least 50 mg/egg) of specific antibodies can be obtained from eggs of these ducks. Interestingly, the comparison of different plasmid constructs showed the important differences in their efficacy of specific IgY antibodies induction in the sera and eggs of immunized ducks.
Abouzid
Karima
K
INSERM Unit 271, 151 Cours Albert Thomas, 69003 Lyon, France.
Ndeboko
Benedicte
B
Durantel
Sandra
S
Jamard
Catherine
C
Zoulim
Fabien
F
Buronfosse
Thierry
T
Cova
Lucyna
L
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D004372:Ducks; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015613:Hepadnaviridae / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins