title: Metallo-beta-lactamase IMP-1 in Providencia rettgeri from two different hospitals in Japan.
abstract: In 2002, 495 indole-positive proteae strains were isolated from patients at 60 hospitals in Japan. Nine indole-positive proteae strains had reduced susceptibility to imipenem (MIC > or = 8 microg ml(-1)) and were identified as Providencia rettgeri by BD Phoenix. Eight of the nine Prov. rettgeri isolates were confirmed as metallo-beta-lactamase producers by the double-disc synergy test. All the metallo-beta-lactamases were classified as IMP-1 by PCR and DNA sequence analysis. These bla(IMP-1) genes were encoded in the integron structure on conjugative plasmids. These plasmids could transfer from Prov. rettgeri clinical isolates to Escherichia coli ML4903 at a frequency between 1.5 x 10(-5) and 5.5 x 10(-7). The eight bla(IMP)-positive strains were isolated from two hospitals, and showed two different PFGE patterns, two different integron structures and two different incompatibility groups, which corresponded to the two hospitals. These results strongly suggest the possibility of nosocomial infections by bla(IMP-1)-producing Prov. rettgeri isolates.
authors.affiliation: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 143-8540 Tokyo, Japan 2Institute of Tropical Medicine, Nagasaki University, Japan 3Department of Clinical Laboratory, Mie University Hospital, Japan.
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Shiroto
authors.affiliation: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 143-8540 Tokyo, Japan 2Institute of Tropical Medicine, Nagasaki University, Japan 3Department of Clinical Laboratory, Mie University Hospital, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ishii
authors.affiliation: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 143-8540 Tokyo, Japan 2Institute of Tropical Medicine, Nagasaki University, Japan 3Department of Clinical Laboratory, Mie University Hospital, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Kimura
authors.affiliation: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 143-8540 Tokyo, Japan 2Institute of Tropical Medicine, Nagasaki University, Japan 3Department of Clinical Laboratory, Mie University Hospital, Japan.
authors.forename: Jimena
authors.initials: J
authors.lastname: Alba
authors.affiliation: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 143-8540 Tokyo, Japan 2Institute of Tropical Medicine, Nagasaki University, Japan 3Department of Clinical Laboratory, Mie University Hospital, Japan.
authors.forename: Kiwao
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 143-8540 Tokyo, Japan 2Institute of Tropical Medicine, Nagasaki University, Japan 3Department of Clinical Laboratory, Mie University Hospital, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Matsushima
authors.affiliation: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 143-8540 Tokyo, Japan 2Institute of Tropical Medicine, Nagasaki University, Japan 3Department of Clinical Laboratory, Mie University Hospital, Japan.
authors.forename: Keizo
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003227:Conjugation, Genetic; D003428:Cross Infection / Q000382:microbiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D032023:Integrons / Q000235:genetics; D007564:Japan; D017720:Molecular Epidemiology; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011532:Providencia / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015378:Imipenem; D001618:beta-Lactamases