title: A proteomic analysis of murine bone marrow and its response to ionizing radiation.
abstract: To characterize the mouse bone marrow tissue proteome and investigate the response to radiation damage we took bone marrow before and after 4-Gy gamma-irradiation from mouse strains (C57BL/6 and CBA/Ca) that differ in their short-term and long-term radiation responses and analyzed extracellular proteins by high-resolution 2-DE. Twenty proteins were identified from 71 protein spots in both C57BL/6 and CBA/Ca. We detected significant differences between control and irradiated bone marrow and between genotypes and identified many of the changed proteins by MS. In C57BL/6, 27 spots were significantly different between control and irradiated samples. In CBA/Ca, 18 spots showed significant changes following irradiation. Proteins such as serum albumin, apolipoprotein A-I, ferritin, haptoglobin (Hp) and alpha-1-antitrypsin were changed in irradiated bone marrow of both mouse strains, reflecting an ongoing acute-phase reaction. Several other proteins including serotransferrin, neutrophil collagenase, peroxiredoxin 2 and creatine kinase M chain were changed specifically in an individual mouse strain. The proteomic approach makes an important contribution to characterizing bone marrow proteome and investigating the tissue response of bone marrow to radiation, assists in identifying genotype-dependent responses and provides support for the importance of microenvironmental factors contributing to the overall response.
authors.affiliation: Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Hospital and Medical School, Dundee, Scotland. c.chen@dundee.ac.uk
authors.forename: Changwei
authors.initials: C
authors.lastname: Chen
authors.forename: Sally A
authors.initials: SA
authors.lastname: Lorimore
authors.forename: Caroline A
authors.initials: CA
authors.lastname: Evans
authors.forename: Antony D
authors.initials: AD
authors.lastname: Whetton
authors.forename: Eric G
authors.initials: EG
authors.lastname: Wright
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism* / Q000528:radiation effects; D015180:Electrophoresis, Gel, Two-Dimensional; D005720:Gamma Rays; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D020543:Proteome / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D020543:Proteome