title: T helper cell-mediated interferon-gamma expression after human parvovirus B19 infection: persisting VP2-specific and transient VP1u-specific activity.
abstract: Human parvovirus B19 is a small non-enveloped DNA virus with an icosahedral capsid consisting of proteins of only two species, the major protein VP2 and the minor protein VP1. VP2 is contained within VP1, which has an additional unique portion (VP1u) of 227 amino acids. We determined the ability of eukaryotically expressed parvovirus B19 virus-like particles consisting of VP1 and VP2 in the ratio recommended for vaccine use, or of VP2 alone, to stimulate, in an HLA class II restricted manner, peripheral blood mononuclear cells (PBMC) to proliferate and to secrete interferon gamma (IFN-gamma) and interleukin (IL)-10 cytokines among recently and remotely B19 infected subjects. PBMC reactivity with VP1u was determined specifically with a prokaryotically expressed VP1u antigen. In general, B19-specific IFN-gamma responses were stronger than IL-10 responses in both recent and remote infection; however, IL-10 responses were readily detectable among both groups, with the exception of patients with relapsed or persisting symptoms who showed strikingly low IL-10 responses. Whereas VP1u-specific IFN-gamma responses were very strong among the recently infected subjects, the VP1u-specific IFN-gamma and IL-10 responses were virtually absent among the remotely infected subjects. The disappearance of VP1u-specific IFN-gamma expression is surprising, as B-cell immunity against VP1u is well maintained.
authors.affiliation: Department of Virology, Haartman Institute and HUCH Diagnostic, University of Helsinki, Finland. rauli.franssila@helsinki.fi
authors.forename: R
authors.initials: R
authors.lastname: Franssila
authors.forename: J
authors.initials: J
authors.lastname: Auramo
authors.forename: S
authors.initials: S
authors.lastname: Modrow
authors.forename: M
authors.initials: M
authors.lastname: Möbs
authors.forename: C
authors.initials: C
authors.lastname: Oker-Blom
authors.forename: P
authors.initials: P
authors.lastname: Käpylä
authors.forename: M
authors.initials: M
authors.lastname: Söderlund-Venermo
authors.forename: K
authors.initials: K
authors.lastname: Hedman
mesh_terms: D000328:Adult; D036022:Capsid Proteins / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D004731:Endotoxins / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology*; D016753:Interleukin-10 / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000276:immunology*; D016732:Parvovirus B19, Human / Q000276:immunology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D036022:Capsid Proteins; D004731:Endotoxins; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D016753:Interleukin-10; D007371:Interferon-gamma