Chemical stabilization of the phycocyanin from cyanobacterium Spirulina platensis.
Phycocyanin (PC) prepared from a cyanobacterium Spirulina platensis by the DEAE-DE52 cellulose column chromatography that was developed by gradient elution of 50-250 mM phosphate buffer (pH 7.0) was stabilized by its subunits cross-linked covalently with formaldehyde. The single blue band that the chemically stabilized PC showed in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that the stabilized PC still maintained its trimeric aggregate form even after its incubation at 60 degrees C for 3h and at 100 degrees C for 10 min in the denatured buffer containing 5% (w/v) SDS. Moreover, the stabilized PC exhibited similar spectroscopic properties of absorption and fluorescence to those of the native PC, and showed adequate energy coupling with R-phycoerythrin (R-PE) after it was conjugated with R-PE via glutaraldehyde.
Sun
Li
L
College of Chemical and Biological Sciences and Engineering, Yantai University, Shandong 264005, PR China. sunlwang@public.ytptt.sd.cn
Wang
Shumei
S
Qiao
Zhiyong
Z
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange / Q000379:methods; D000458:Cyanobacteria / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D010798:Phycocyanin / Q000737:chemistry* / Q000302:isolation & purification; D010799:Phycoerythrin / Q000737:chemistry
D001426:Bacterial Proteins; D010798:Phycocyanin; D010799:Phycoerythrin