[Detection of multiresistance Aeromonas with TEM type resistant genes in patients with cirrhosis].
OBJECTIVE
To study the status of beta-lactamase produced by multiresistant Aeromonas selected from cirrhosis patients to provide reference for treatment and reduce resistance and control spreading.


METHODS
Four multiresistant Aeromonas strains isolated from serious liver cirrhosis patients from the No. 302 hospital. The TEM resistant genes were detected by PCR and agarose gel electrophoresis.


RESULTS
Three TEM-1 positive strains were detected from four multiresistant Aeromonas isolates consisting of one Aeromonas sobria and three Aeromonas hydrophila isolated from blood and ascites. This was further confirmed by gene sequencing. The multiresistance to antibiotics was higher in four Aeromonas isolates. All strains tested were resistant to ampicillin, cefazolin and cefmetazole.The cirrhosis patients who suffered from Aeromonas infection had poor prognosis and had mortality rate of 3/4.


CONCLUSIONS
The beta-lactamase TEM-1 resistant genes was detected in clinical multiresistant Aeromonas strain isolated from serious cirrhosis patients.The results suggested that TEM-1 was the main resistance mechanism of Aeromonas strain and was reduced by adding enzyme inhibitor.
Qu
Fen
F
The Center of Clinical Laboratory, The No 302 Hospital of the People's Liberation Army, Beijing, China.
Bao
Chun-mei
CM
Cui
En-bo
EB
Shi
Jia-bin
JB
Guo
Tong-sheng
TS
Mao
Yuan-li
YL
D000328:Adult; D000333:Aeromonas / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008103:Liver Cirrhosis / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D001618:beta-Lactamases / Q000235:genetics
D000900:Anti-Bacterial Agents; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1