title: Keratinophilic fungi recovered from muddy soil in Cairo vicinities, Egypt.
abstract: One hundred samples of muddy soil were collected from seven areas in the vicinity of Cairo and screened for the presence of keratinophilic fungi by using hair baiting isolation technique. Forty isolates of keratinophilic fungi were recovered and identified by recognition of their cultures, macro- and micromorphological features. Their physiological and molecular characteristics were studied by determination of their ubiquinone (Coenzyme Q) composition and DNA sequences of (ITS1-5.8S-ITS2) and 18S rRNA region sequences. The Keratinophilic isolates were identified as Chrysosporium carmichaelii, C. queenslandicum, C. zonatum, C. indicum, Aphanoascus mephitalis, and Uncinocarpus reesii. Chrysosporium zonatum was the most prevalent species and represented 42.5% of the total number of isolates. Each of C. carmichaelii and C. queenslandicum were equal in their prevalence and represented 15%. C. indicum comes next constituting 12.5%; followed by Uncinocarpus reesii which represented 10%. The least prevalent species in our study was Aphanoascus mephitalis, which was represented only 5% of the total keratinophilic isolates.
authors.affiliation: Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt. shzaki@yahoo.com
authors.forename: S M
authors.initials: SM
authors.lastname: Zaki
authors.forename: Y
authors.initials: Y
authors.lastname: Mikami
authors.forename: A A Karam
authors.initials: AA
authors.lastname: El-Din
authors.forename: Y A
authors.initials: YA
authors.lastname: Youssef
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002912:Chrysosporium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D004534:Egypt; D006197:Hair / Q000382:microbiology; D007633:Keratins / Q000378:metabolism*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S; D014451:Ubiquinone; D007633:Keratins