Backbone 1H, 15N, and 13C resonance assignment of HP1242 from Helicobacter pylori.
One of the small proteins from Helicobacter pylori, HP1242, was investigated by the solution nuclear magnetic resonance (NMR) spectroscopy. HP1242 is known as a 76-residue conserved hypothetical protein and its function cannot be identified based on sequence homology. Here, the results of the backbone (1)H, (15)N, and (13)C resonance assignments of the HP1242 are reported using double- and triple-resonance techniques. About 95 % of all of the (1)HN, (15)N, (13)CO, (13)Calpha, and (13)Cbeta resonances that cover 75 non-Proline residues of the 76 residues are clarified through sequential- and specific- assignments. In addition, three helical regions were clearly identified on the basis of the resonance assignments.
Kang
Su-Jin
SJ
National Lab. of Membrane Protein Structure (MPS), Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Korea.
Park
Sung Jean
SJ
Jung
Seo-Jeong
SJ
Lee
Bong-Jin
BJ
D001426:Bacterial Proteins / Q000737:chemistry*; D016480:Helicobacter pylori / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D016366:Open Reading Frames; D017433:Protein Structure, Secondary
D001426:Bacterial Proteins