A simple method of isolation of chloramphenicol in honey and its estimation by liquid chromatography coupled to electrospray ionization tandem mass spectrometry.
To detect sub-ppb levels of the antibiotic chloramphenicol in honey matrix, a convenient method of extraction and measurement using liquid chromatography with detection by tandem mass spectrometry (LC/MS/MS) was developed. Honey samples fortified with chloramphenicol and isotopically labeled chloramphenicol were extracted using diatomaceous-based supported liquid-liquid extraction cartridges to generate a standard calibration curve. Four MS/MS transitions were used for quantification and four other transitions for confirmation of chloramphenicol. The limit of detection for chloramphenicol was 0.05 ng/g and the lower limit of quantification was 0.1 ng/g. Several commercial honey samples were analyzed for chloramphenicol content using this method.
Vivekanandan
Kannan
K
Dabur Research Foundation, 22 Site IV, Sahibabad, UP 201010, India. vivekanandank@dabur.com
Swamy
Madugundu Guru
MG
Prasad
Sudhanand
S
Mukherjee
Rama
R
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002701:Chloramphenicol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis*; D006722:Honey; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000900:Anti-Bacterial Agents; D002701:Chloramphenicol