[Preliminary analysis of mutations in exon 5 of IkappaBalpha gene in Hodgkin lymphoma].
OBJECTIVE
To study the mutations in exon 5 of IkappaBalpha gene and their significance in Hodgkin lymphoma.


METHODS
CD30-positive Reed-Sternberg cells and surrounding reactive lymphocytes were selected by laser microdissection technology from 6 microm-thick histologic sections of 7 Hodgkin lymphoma cells studied. Genomic DNA of these cells was extracted according to Qiagen micro DNAkit guide. Semi-nested polymerase chain reaction (PCR) and direct DNA sequencing were used to analyze exon 5 of IkappaBalpha gene.


RESULTS
A mutation in exon 5 was identified in 3 of the 7 Hodgkin lymphoma cases. No mutation found in the surrounding reactive lymphocytes. The mutation site was located at codon 2084 (T-A, NT_026437), resulting in formation of a stop codon.


CONCLUSIONS
The mutation identified in exon 5 of the IkappaBalpha gene in Hodgkin lymphoma may play a role in the pathogenesis of Hodgkin lymphoma.
Liu
Xiao-jian
XJ
Department of Molecular Pathology, Cancer Hospital, Fudan University, Shanghai 200032, China.
Yang
Wen-tao
WT
Zhou
Xiao-yan
XY
Yan
Qing
Q
Shi
Da-ren
DR
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D006689:Hodgkin Disease / Q000235:genetics*; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D000072000:NF-KappaB Inhibitor alpha; D017354:Point Mutation
D004273:DNA, Neoplasm; D020672:I-kappa B Proteins; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha