title: Bovine PrPC directly interacts with alphaB-crystalline.
abstract: We used a bovine brain cDNA library to perform a yeast two-hybrid assay with bovine mature PrP(C) as bait. The screening result showed that alphaB-crystalline interacted with PrP(C). The interaction was further evaluated both in vivo and in vitro with different methods, such as immunofluorescent colocalization, native polyacrylamide-gel electrophoresis, and IAsys biosensor assays. The results suggested that alphaB-crystalline may have the ability to refold denatured prion proteins, and provided first evidence that alphaB-crystalline is directly associated with prion protein.
authors.affiliation: The Modern Virology Research Centre and State Key Laboratory of Virology, College of Life Sciences, Wuhan University, PR China.
authors.forename: Guihong
authors.initials: G
authors.lastname: Sun
authors.forename: Mingxiong
authors.initials: M
authors.lastname: Guo
authors.forename: Ao
authors.initials: A
authors.lastname: Shen
authors.forename: Fanghua
authors.initials: F
authors.lastname: Mei
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Peng
authors.forename: Rui
authors.initials: R
authors.lastname: Gong
authors.forename: Deyin
authors.initials: D
authors.lastname: Guo
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wu
authors.forename: Po
authors.initials: P
authors.lastname: Tien
authors.forename: Gengfu
authors.initials: G
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002417:Cattle; D003459:Crystallins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D018621:PrPC Proteins / Q000378:metabolism*; D011485:Protein Binding; D017510:Protein Folding; D020798:Two-Hybrid System Techniques
chemical_list: D003459:Crystallins; D018621:PrPC Proteins