[Analysis of the functions of wheat resistance-related genes by a transient expression system].
Transient expression system was used to analyze the functions of three resistance- related genes: TaTBL, TaPK1 and TaTST. Target genes were constructed into plant expression vectors and transformed into leaf epidermal cells of a powdery mildew-susceptible wheat variety by gene gun. GUS gene was co-transformed with target gene to mark the transformed cells. After transformation, leaf surface was inoculated with powdery mildew conidiospores. Forty eight hours after inoculation, penetration of the fungus and formation of haustoria in transformed cells were observed to evaluate the effects of the target gene's products on the invasion of powdery mildew. The results implied that all these three genes, when transiently expressed in leaf epidermal cells of susceptible wheat variety, could partly inhibit the penetration of conidiospores and formation of haustoria, and to some extent increase the resistance of cells to powdery mildew.
Wang
Hua-Zhong
HZ
The National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Niu
Ji-Shan
JS
Chen
Pei-Du
PD
D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D001203:Ascomycota / Q000254:growth & development; D000071186:Beclin-1; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007113:Immunity, Innate / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D008856:Microscopy, Fluorescence; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D019441:Plant Epidermis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013884:Thiosulfate Sulfurtransferase / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology
D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D017346:Protein Serine-Threonine Kinases; D013884:Thiosulfate Sulfurtransferase; D005966:Glucuronidase