title: Detection of water-borne E. coli O157 using the integrating waveguide biosensor.
abstract: Escherichia coli O157:H7, the most common serotype of enterohemorrhagic E. coli (EHEC), is responsible for numerous food-borne and water-borne infections worldwide. An integrating waveguide biosensor is described for the detection of water-borne E. coli O157, based on a fluorescent sandwich immunoassay performed inside a glass capillary waveguide. The genomic DNA of captured E. coli O157 cells was extracted and quantitative real-time PCR subsequently performed to assess biosensor-capture efficiency. In vitro microbial growth in capillary waveguide is also documented. The biosensor allows for quantitative detection of as few as 10 cells per capillary (0.075 ml volume) and can be used in conjunction with cell amplification, PCR and microarray technologies to positively identify a pathogen.
authors.affiliation: Creatv MicroTech, Inc., 11609 Lake Potomac Drive, Potomac, MD 20854, USA. pzhu@creatvmicrotech.com
authors.forename: Peixuan
authors.initials: P
authors.lastname: Zhu
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Shelton
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Karns
authors.forename: Appavu
authors.initials: A
authors.lastname: Sundaram
authors.forename: Shuhong
authors.initials: S
authors.lastname: Li
authors.forename: Pete
authors.initials: P
authors.lastname: Amstutz
authors.forename: Cha-Mei
authors.initials: CM
authors.lastname: Tang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D015169:Colony Count, Microbial / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018511:Systems Integration