Calcium triggers folding of lipoprotein lipase into active dimers.
The active form of lipoprotein lipase (LPL) is a noncovalent homodimer of 55-kDa subunits. The dimer is unstable and tends to undergo irreversible dissociation into inactive monomers. We noted that a preparation of such monomers slowly regained traces of activity under assay conditions with substrate, heparin, and serum or in cell culture medium containing serum. We therefore studied the refolding pathway of LPL after full denaturation in 6 M guanidinium chloride or after dissociation into monomers in 1 M guanidinium chloride. In crude systems, we identified serum as the factor promoting reactivation. Further investigations demonstrated that Ca2+ was the crucial component in serum for reactivation of LPL and that refolding involved at least two steps. Studies of far-UV circular dichroism, fluorescence, and proteolytic cleavage patterns showed that LPL started to refold from the C-terminal domain, independent of calcium. The first step was rapid and resulted in formation of an inactive monomer with a completely folded C-terminal domain, whereas the N-terminal domain was in the molten globule state. The second step was promoted by Ca2+ and converted LPL monomers from the molten globule state to dimerization-competent and more tightly folded monomers that rapidly formed active LPL dimers. The second step was slow, and it appears that proline isomerization (rather than dimerization as such) is rate-limiting. Inactive monomers isolated from human tissue recovered activity under the influence of Ca2+. We speculate that Ca2+-dependent control of LPL dimerization might be involved in the normal post-translational regulation of LPL activity.
Zhang
Liyan
L
Department of Medical Biosciences, Physiological Chemistry, Umeå University, SE-901 87 Umeå, Sweden.
Lookene
Aivar
A
Wu
Gengshu
G
Olivecrona
Gunilla
G
D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002645:Chickens; D002845:Chromatography / Q000379:methods; D002942:Circular Dichroism; D003470:Culture Media / Q000378:metabolism; D021984:Cyclophilin A / Q000494:pharmacology; D019281:Dimerization; D015971:Gene Expression Regulation, Enzymologic; D019791:Guanidine / Q000494:pharmacology; D006493:Heparin / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D010920:Placenta / Q000378:metabolism; D011485:Protein Binding; D011489:Protein Denaturation; D017510:Protein Folding; D011499:Protein Processing, Post-Translational; D020673:Protein Renaturation; D017434:Protein Structure, Tertiary; D012685:Sepharose / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology; D014466:Ultraviolet Rays
D003470:Culture Media; D013395:Sucrose; D006493:Heparin; D012685:Sepharose; D008071:Lipoprotein Lipase; D014357:Trypsin; D021984:Cyclophilin A; D019791:Guanidine; D002118:Calcium