Regulation of proliferation, motility, and contractivity of cultured human endometrial stromal cells by transforming growth factor-beta isoforms.
OBJECTIVE
To evaluate the involvement of transforming growth factor (TGF)-beta isoforms (TGF-beta1, TGF-beta2, and TGF-beta3) on endometrial tissue remodeling during the perimenstrual period.


METHODS
The effects of TGF-beta isoforms on the cell proliferation, motility, and contractivity of cultured human endometrial stromal cells (ESCs) were investigated.


METHODS
Research laboratory at a medical school.


METHODS
Nine endometrial specimens in the late secretory phase were used.


METHODS
Endometrial stromal cells were incubated with recombinant human recombinant TGF-beta1, TGF-beta2, and TGF-beta3.


METHODS
The cell proliferation, motility, and contractivity of ESCs were accessed by a modified methylthiazoletetrazolium assay, in vitro wound repair assay, transwell invasion assay, and collagen gel contraction assay.


RESULTS
All three isoforms of TGF-beta significantly inhibited the cell proliferation of ESCs in a dose-dependent manner. In vitro wound repair assay and transwell invasion assay demonstrated that the TGF-beta isoforms significantly inhibited the motility of ESCs. However, the TGF-beta isoforms were shown to have a clear effect on the collagen gel contractivity of ESCs.


CONCLUSIONS
These results suggest that TGF-beta isoforms may promote endometrial tissue repair through the inhibition of the proliferation, expansion, and migration of ESCs, and through the stimulation of the contraction of the collagen gel matrix by these cells. Transforming growth factor-beta may be involved in the protection of the endometrium from extensive fibrosis and scarring by regulating ESC function during the perimenstrual period.
Nasu
Kaei
K
Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Oita, Japan. nasu@med.oita-u.ac.jp
Nishida
Masakazu
M
Matsumoto
Harunobu
H
Bing
Sun
S
Inoue
Chieko
C
Kawano
Yasushi
Y
Miyakawa
Isao
I
D000328:Adult; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D048429:Cell Size; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology* / Q000502:physiology*; D005260:Female; D006131:Growth Inhibitors / Q000502:physiology*; D006801:Humans; D020033:Protein Isoforms / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000502:physiology*; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2; D053782:Transforming Growth Factor beta3
D006131:Growth Inhibitors; D020033:Protein Isoforms; C508960:TGFB1 protein, human; C509041:TGFB2 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2; D053782:Transforming Growth Factor beta3