Apoptosis role of FAS/FAS ligand system in the regulation of myelopoiesis.
OBJECTIVE
CD34+ cells and colony forming unit-granulocyte and macrophage (CFU-GM) from human bone marrow were used to investigate the role of Fas/FasL system in the regulation of myelopoiesis.


METHODS
Fas and FasL expression in CD34+ cells and in day 14 CFU-GM were measured by RT-PCR and immunofluorescence respectively. The functional assays for the CFU-GM were measured by a standard colony assay and the proliferative capacity of CFU-GM was measured by replating the primary colony and observing the secondary colony formation. Human marrow cells were treated with IETD (caspase-8 inhibitor) or anti-Fas CH-11 Mab.


RESULTS
Treatment with the CFU-GM with IETD significantly increased, the proliferative capacity, while anti-Fas CH-11 Mab markedly reduced it. Fas and FasL expression were demonstrated using RT-PCR and immunofluorescence respectively.


CONCLUSIONS
Fas, FasL, and caspase activation are likely to play an important role in the regulation of myelopoiesis.
Alenzi
Faris Q
FQ
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, King Faisal University, Dammam, Saudi Arabia. falenzi@kfu.edu.sa
Al-Ghamdi
Saleh M
SM
Tamimi
Waleed G
WG
Al-Sebiany
Abdulaziz M
AM
El-Nashar
Iman M
IM
El-Tounsi
Iman
I
Bamaga
Mohammad S
MS
Al-Enazi
Maher M
MM
Al-Amri
Ali S
AS
Al-Sheikh
Iman H
IH
D018952:Antigens, CD34 / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D053222:Fas Ligand Protein; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D038042:Myelopoiesis / Q000502:physiology*; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism*; D048069:Tumor Necrosis Factors / Q000378:metabolism*; D019014:fas Receptor
D018952:Antigens, CD34; C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D018124:Receptors, Tumor Necrosis Factor; D048069:Tumor Necrosis Factors; D019014:fas Receptor