Identification of botanicals and potential contaminants through RFLP and sequencing.
Botanical supplements for health enhancement are being increasingly used in the United States, but no safeguards are formally in place to ensure that they are not contaminated with non-efficacious or potentially harmful plant material. A molecular approach, which allows the authentication of botanical ingredients and detection of contaminating plant material by analyzing the ITS-1 region by PCR-RFLP and subsequent sequencing, is described. When using starting material from which DNA can be obtained, this method has the potential for identifying both primary and contaminating plant material in botanical dietary supplements.
Lum
Michelle R
MR
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095-1606, USA.
Potter
Ellie
E
Dang
Thanh
T
Heber
David
D
Hardy
Mary
M
Hirsch
Ann M
AM
D001483:Base Sequence; D018744:DNA, Plant / Q000032:analysis*; D006801:Humans; D000455:Medicago sativa / Q000737:chemistry; D008969:Molecular Sequence Data; D008517:Phytotherapy / Q000592:standards*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011237:Predictive Value of Tests; D029921:Trifolium / Q000737:chemistry
D018744:DNA, Plant