title: ADAM12 overexpression does not improve outcome in mice with laminin alpha2-deficient muscular dystrophy.
abstract: We have recently shown that overexpression of ADAM12 results in increased muscle regeneration and significantly reduced pathology in mdx, dystrophin deficient mice. In the present study, we tested the effect of overexpressing ADAM12 in dy(W) laminin-deficient mice. dy mice have a very severe clinical phenotype and would be expected to benefit greatly from enhanced regeneration. We found that dy(W) mice overexpressing ADAM12 indeed have increased muscle regeneration, as evidenced by increased numbers of muscle fibers expressing fetal myosin. However, overexpression of ADAM12 had no significant effect on overall health, as evidenced by body weight, and did not improve muscle pathology.
authors.affiliation: The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Ling T
authors.initials: LT
authors.lastname: Guo
authors.forename: G Diane
authors.initials: GD
authors.lastname: Shelton
authors.forename: Ulla M
authors.initials: UM
authors.lastname: Wewer
authors.forename: Eva
authors.initials: E
authors.lastname: Engvall
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D000072199:ADAM12 Protein; D000367:Age Factors; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001835:Body Weight / Q000502:physiology; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D007797:Laminin / Q000172:deficiency*; D051379:Mice; D018345:Mice, Knockout; D009137:Muscular Dystrophy, Animal / Q000235:genetics / Q000378:metabolism*; D012038:Regeneration / Q000235:genetics / Q000502:physiology
chemical_list: D007797:Laminin; C102645:laminin alpha 2; D051722:ADAM Proteins; D000072199:ADAM12 Protein; C096115:Adam12 protein, mouse