Adsorption, desorption and activities of acid phosphatase on various colloidal particles from an Ultisol.
Adsorption, desorption and activity of acid phosphatase on various soil colloidal particles and pure clay minerals were studied. Higher adsorption amounts and low percentage of desorption of acid phosphatase were found on fine soil clays (<0.2 microm). Electrostatic force and ligand exchange are the major driving forces that are involved in the adsorption of enzymes on soil clays. More enzyme molecules were adsorbed on soil clays in the presence of organic components. However, enzymes on organic clays were more easily released. One-third of the enzyme on goethite was adsorbed via ligand exchange process. Some other interactions, such as van der Waals force, hydrophobic force and hydrogen bonding may be more important in the adsorption of enzyme on kaolinite and the enzyme in this system cannot be easily removed. Coarse clays (0.2-2 microm) and inorganic soil clays had higher affinities for enzyme molecules than fine clays and organic clays, respectively. The activity of enzyme bound on soil clays was inhibited and the thermal stability was increased in the presence of organic matter. Data obtained in this study are helpful for a better understanding of the interactions of enzymes with inorganic and organic constituents in soil and associated environments.
Huang
Qiaoyun
Q
State Key Laboratory of Agricultural Microbiology, Faculty of Resources and Environment, Huazhong Agriculture University, Wuhan 430070, China. qyhuang@mail.hzau.edu.cn
Liang
Wei
W
Cai
Peng
P
D000135:Acid Phosphatase / Q000737:chemistry*; D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D000077215:Clay; D003102:Colloids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D058085:Iron Compounds / Q000737:chemistry; D007616:Kaolin / Q000737:chemistry; D008024:Ligands; D008903:Minerals; D010316:Particle Size; D012680:Sensitivity and Specificity; D012987:Soil; D013499:Surface Properties; D013696:Temperature
D000538:Aluminum Silicates; D003102:Colloids; D058085:Iron Compounds; D008024:Ligands; D008903:Minerals; D012987:Soil; C094886:goethite; D007616:Kaolin; D000135:Acid Phosphatase; D000077215:Clay