title: High-throughput sequencing of high copy number plasmids from bacterial cultures by heat lysis.
authors.affiliation: DNA Sequencing facility, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA. gangulyt@mail.med.upenn.edu
authors.forename: Tapan
authors.initials: T
authors.lastname: Ganguly
authors.forename: Peiqin
authors.initials: P
authors.lastname: Chen
authors.forename: Rebecca
authors.initials: R
authors.lastname: Teetsel
authors.forename: Lan Ping
authors.initials: LP
authors.lastname: Zhang
authors.forename: Elias
authors.initials: E
authors.lastname: Papaioannou
authors.forename: Joseph
authors.initials: J
authors.lastname: Cianciarulo
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002458:Cell Fractionation / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D018628:Gene Dosage; D006358:Hot Temperature; D010957:Plasmids / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004269:DNA, Bacterial