title: [cDNA microarray and cluster analysis to identify the significance of immune genes associated with benzene poisoning].
abstract: OBJECTIVE
  To delineate the immune regulatory pathway of benzene poisoning by using gene expression profile analysis.
  
  
  METHODS
  Peripheral white blood cell gene expression profile of 7 benzene poisoning patients, including one aplastic anemia, was determined by microarray. Seven chips from normal workers were served as controls. Cluster analysis of gene expression profile was performed. Differentially expressed immune genes associated with benzene poisoning were determined.
  
  
  RESULTS
  Among the 2 779 target genes, 38 genes differentially expressed were identified, including 10 up-regulated genes such as CD59, TRA@, MCP etc, and 14 down-regulated genes such as HLA-DMB, HLA-DQA1, HLA-DPB1, ITGB2, PFC etc. Cluster analysis showed that the expression profiles of 38 genes were associated with benzene poisoning.
  
  
  CONCLUSIONS
  Differentially expressed immune genes may play an important role in the pathogenesis of benzene poisoning.
authors.affiliation: Department of Occupational and Enironmental Health, School of Public Health, Wuhan University, Wuhan, 430071, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Bi
authors.forename: Chunhong
authors.initials: C
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Tao
authors.forename: Ying
authors.initials: Y
authors.lastname: Xia
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001554:Benzene / Q000506:poisoning*; D018957:CD59 Antigens / Q000235:genetics; D016022:Case-Control Studies; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D006681:HLA-D Antigens / Q000235:genetics; D059868:HLA-DP beta-Chains / Q000235:genetics; D059848:HLA-DQ alpha-Chains / Q000235:genetics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D018957:CD59 Antigens; D006681:HLA-D Antigens; C084245:HLA-DM antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D001554:Benzene