title: Air-liquid interface culture of serially passaged human nasal epithelial cell monolayer for in vitro drug transport studies.
abstract: The objective of this study was to establish a drug transport study using human nasal epithelial (HNE) cell monolayers cultured by the air-liquid interface (ALI) method using serum-free medium (BEGM:DME/F12, 50:50). The cells were developed and characterized in comparison to those that have been previously cultured by the liquid-covered culture (LCC) method. The epithelial cell monolayer cultured by the ALI method resulted in a significantly higher transepithelial electrical resistance value (3,453 +/- 302 ohm x cm(2)) that was maintained (>1,000 ohm x cm(2)) for up to 20 days compared with that cultured by the LCC method. Observation by scanning electron microscopy revealed mature cilia after 2 weeks in the ALI culture, while flatten unhealthy ciliated cells were observed in the LCC method. After 21 days, higher level of MUC5AC and 8 mRNA were expressed in ALI culture which confirmed the secretory differentiation of HNE monolayers in vitro. No significant difference in the permeability coefficients of a model hydrophilic marker ((14)C-mannitol) and a lipophilic drug (budesonide) was observed between the two conditions on day 7. The passage 2-3 of the HNE monolayer using ALI condition retained the morphology and differentiated features of normal epithelium. Thus it would be a suitable model for in vitro nasal drug delivery studies.
authors.affiliation: College of Medicine, Pusan National University, Busan, South Korea.
authors.forename: Min-Ki
authors.initials: MK
authors.lastname: Lee
authors.forename: Jin-Wook
authors.initials: JW
authors.lastname: Yoo
authors.forename: Hongxia
authors.initials: H
authors.lastname: Lin
authors.forename: You-Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: Dae-Duk
authors.initials: DD
authors.lastname: Kim
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Choi
authors.forename: Soon-Kew
authors.initials: SK
authors.lastname: Park
authors.forename: Chang-Hun
authors.initials: CH
authors.lastname: Lee
authors.forename: Hwan-Jung
authors.initials: HJ
authors.lastname: Roh
mesh_terms: D001692:Biological Transport; D018929:Cell Culture Techniques; D017097:Electric Impedance; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009297:Nasal Mucosa / Q000166:cytology* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction