title: Metal-specific differences in levels of DNA damage caused by synovial fluid recovered at revision arthroplasty.
abstract: Previous research has shown an increase in chromosomal aberrations in patients with worn implants. The type of aberration depended on the type of metal alloy in the prosthesis. We have investigated the metal-specific difference in the level of DNA damage (DNA stand breaks and alkali labile sites) induced by culturing human fibroblasts in synovial fluid retrieved at revision arthroplasty. All six samples from revision cobalt-chromium metal-on-metal and four of six samples from cobalt-chromium metal-on-polyethylene prostheses caused DNA damage. By contrast, none of six samples from revision stainless-steel metal-on-polyethylene prostheses caused significant damage. Samples of cobalt-chromium alloy left to corrode in phosphate-buffered saline also caused DNA damage and this depended on a synergistic effect between the cobalt and chromium ions. Our results further emphasise that epidemiological studies of orthopaedic implants should take account of the type of metal alloy used.
authors.affiliation: Bristol Implant Research Centre, Southmead Hospital, Bristol BS10 5NB, UK. andypdavies@hotmail.com
authors.forename: A P
authors.initials: AP
authors.lastname: Davies
authors.forename: A
authors.initials: A
authors.lastname: Sood
authors.forename: A C
authors.initials: AC
authors.lastname: Lewis
authors.forename: R
authors.initials: R
authors.lastname: Newson
authors.forename: I D
authors.initials: ID
authors.lastname: Learmonth
authors.forename: C P
authors.initials: CP
authors.lastname: Case
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019643:Arthroplasty, Replacement; D002478:Cells, Cultured; D002858:Chromium Alloys; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007595:Joint Prosthesis; D008422:Materials Testing / Q000379:methods; D008670:Metals / Q000633:toxicity*; D008875:Middle Aged; D011474:Prosthesis Design; D013193:Stainless Steel / Q000633:toxicity; D013582:Synovial Fluid / Q000737:chemistry*
chemical_list: D002858:Chromium Alloys; D008670:Metals; D013193:Stainless Steel