The immortalized human corneal epithelial cells adhere to laminin-10 by using Lutheran glycoproteins and integrin alpha3beta1.
We studied the adhesion characteristics of immortalized human corneal epithelial (HCE) cells on purified human laminin (Ln)-10 (alpha5beta1gamma1). Immunofluorescence studies with monoclonal antibodies (MAb) revealed the presence of laminin alpha5 chain receptor Lutheran (Lu) in vivo on the basal cells of cornea and as punctate cell surface-confined reactivity on adhering and spread HCE cells, while alpha- and beta-dystroglycans could not be found. Northern blot analysis showed the presence of 4.0 and 2.8 kb Lu transcripts in HCE cells. The results showed that Ln-10 induced adhesion and spreading of HCE cells without formation of focal adhesions. Quantitative adhesion assay showed that Lu together with integrin (Int) alpha3beta1 independently mediated the adhesion of HCE cells to Ln-10.
Hasenson
Sissi
S
Institute of Biomedicine/Anatomy, University of Helsinki, Helsinki, Finland. mailto:sissi.hasenson@helsinki.fi
Määttä
Marko
M
Rousselle
Patricia
P
Kikkawa
Yamato
Y
Miner
Jeffrey H
JH
Tervo
Timo
T
Virtanen
Ismo
I
D015152:Blotting, Northern; D002448:Cell Adhesion / Q000502:physiology; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D049030:Dystroglycans / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D019573:Epithelium, Corneal / Q000166:cytology / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D006801:Humans; D039201:Integrin alpha3beta1 / Q000502:physiology*; D007797:Laminin / Q000378:metabolism*; D008188:Lutheran Blood-Group System / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics
D039201:Integrin alpha3beta1; D007797:Laminin; D008188:Lutheran Blood-Group System; D012333:RNA, Messenger; C119013:laminin 10; D049030:Dystroglycans