Experimental manipulation of transforming growth factor-beta isoforms significantly affects adhesion formation in a murine surgical model.
Transforming growth factor-beta (TGF-beta), a multifunctional growth factor, represents three mammalian isoforms, TGF-beta1, TGF-beta2, and TGF-beta3. In cutaneous wound healing, combined neutralization of TGF-beta1 and -beta2 or addition of TGF-beta3 reduces scar formation. Here, we investigated whether experimental manipulation of TGF-beta isoforms reduced adhesion formation after injury to the peritoneum. Adhesions were produced in mice by surgical abrasion of adjacent serosa followed by close apposition. In the first part of this study, a detailed analysis of TGF-beta isoform distribution was performed through immunolocalization. TGF-beta isoforms clearly showed a unique temporal and spatial pattern of expression after peritoneal wounding. Based on this pharmacokinetic data, we next administered neutralizing antibodies to TGF-beta1 and -beta2 or exogenous TGF-beta3 peptide by local application and intraperitoneal injection at various times before and after surgery. At day 7 after surgery, addition of neutralizing antibodies to both TGF-beta1 and -beta2 significantly reduced the number and size of adhesions (P < 0.05) compared with the vehicle control. By contrast, exogenous addition of TGF-beta3 either had no effect or increased adhesion formation compared to the vehicle control. In conclusion, these results show that by blocking both TGF-beta1 and TGF-beta2 using neutralizing antibodies, it is possible to prevent abdominal adhesion formation.
Gorvy
Dylan A
DA
Faculty of Life Sciences, University of Manchester, Manchester, UK M13 9PT.
Herrick
Sarah E
SE
Shah
Mamta
M
Ferguson
Mark W J
MW
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology; D002432:Cecum / Q000166:cytology / Q000276:immunology; D007150:Immunohistochemistry; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008953:Models, Anatomic; D009500:Neutralization Tests; D010532:Peritoneal Diseases / Q000209:etiology* / Q000378:metabolism; D010533:Peritoneal Lavage; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D011897:Random Allocation; D000267:Tissue Adhesions / Q000209:etiology / Q000378:metabolism*; D014018:Tissue Distribution; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
D000906:Antibodies; D020033:Protein Isoforms; D016212:Transforming Growth Factor beta