title: Recovery of stem cells from cryopreserved periodontal ligament.
abstract: Human post-natal stem cells possess a great potential to be utilized in stem-cell-mediated clinical therapies and tissue engineering. It is not known whether cryopreserved human tissues contain functional post-natal stem cells. In this study, we utilized human periodontal ligament to test the hypothesis that cryopreserved human periodontal ligament contains retrievable post-natal stem cells. These cryopreserved periodontal ligament stem cells maintained normal periodontal ligament stem cell characteristics, including expression of the mesenchymal stem cell surface molecule STRO-1, single-colony-strain generation, multipotential differentiation, cementum/periodontal-ligament-like tissue regeneration, and a normal diploid karyotype. Collectively, this study provides valuable evidence demonstrating a practical approach to the preservation of solid-frozen human tissues for subsequent post-natal stem cell isolation and tissue regeneration. The present study demonstrates that human post-natal stem cells can be recovered from cryopreserved human periodontal ligament, thereby providing a practical clinical approach for the utilization of frozen tissues for stem cell isolation.
authors.affiliation: Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: B-M
authors.initials: BM
authors.lastname: Seo
authors.forename: M
authors.initials: M
authors.lastname: Miura
authors.forename: W
authors.initials: W
authors.lastname: Sonoyama
authors.forename: C
authors.initials: C
authors.lastname: Coppe
authors.forename: R
authors.initials: R
authors.lastname: Stanyon
authors.forename: S
authors.initials: S
authors.lastname: Shi
mesh_terms: D000328:Adult; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D015925:Cryopreservation; D003739:Dental Cementum / Q000166:cytology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008964:Molar, Third; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology*; D010513:Periodontal Ligament / Q000166:cytology*; D018709:Statistics, Nonparametric; D033581:Stem Cell Transplantation; D014183:Transplantation, Heterologous