Trichophyton eboreum sp. nov. isolated from human skin.
An unusual dermatophyte was isolated from the plantar scales of a human immunodeficiency virus-positive man with tinea pedis. Morphology, physiology, and molecular data provided evidence to support the new species Trichophyton eboreum. This dermatophyte is characterized by rapid growth on common mycological media, a flat powdery off-white colony, formation of clavate microconidia, smooth- and thin-walled cylindrical or club-shaped macroconidia with two to nine cells, the presence of hook-shaped hyphae, the production of cleistothecium-like structures and spiral hyphae in older cultures, positive hair perforation, the absence of pigmentation on potato glucose agar, the absence of a requirement for vitamins, a weak positive urease reaction, no growth at 37 degrees C, resistance to 5% NaCl, resistance to fluconazole, good growth on human epidermal keratin, and the production of various enzymes on different media by the API-ZYM test. More than 5% divergence from any known species of dermatophyte was revealed by sequence analysis of the internal transcribed spacer of the rRNA gene.
Brasch
Jochen
J
Department of Dermatology, University of Schleswig-Holstein, Campus Kiel, Schittenhelmstr.7, D-24105 Kiel, Germany. jbrasch@dermatology.uni-kiel.de
Gräser
Yvonne
Y
D000328:Adult; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D003881:Dermatomycoses / Q000382:microbiology*; D006679:HIV Seropositivity / Q000150:complications; D006801:Humans; D008297:Male; D016533:Mycological Typing Techniques; D010641:Phenotype; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology*; D014008:Tinea Pedis / Q000150:complications; D014249:Trichophyton / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
D004271:DNA, Fungal