Detection of CD5 in B-cell chronic lymphoproliferative diseases by flow cytometry: a strong expression in B-cell chronic lymphocytic leukemia.
OBJECTIVE
CD5 is a T cell marker, aberrantly express in B cell chronic lymphocytic leukemia (B-CLL) and mantle cell lymphoma (MCL). Other chronic B cell malignancies including hairy cell leukemia (HCL) and B cell prolymphocytic leukemia (B-PLL) are CD5 negative or express this antigen in a weak way. In this study, CD5 expression was investigated in leukemic cells from 42 patients with chronic B cell lymphoproliferative disease.


METHODS
We studied the CD5 expression in leukemic cells from 42 patients with chronic B-cell malignancies by flow cytometry. Demographic features such as age, sex and clinical date were also analyzed.


RESULTS
There were 22 males and 20 females. The immunophenotyping showed that 35 cases were B-CLL, 3 B-PLL and HCL and one patient was MCL. CD5 expression was present in all B-CLL and MCL. Low expression of CD5 was observed in one patient with B-PLL and negative in all cases of HCL.


CONCLUSIONS
Our date demonstrated that CD5 expression can help distinguish among B-CLL from HCL and B-PLL, but is similar expressed in MCL.
Cavalcanti Júnior
Geraldo Barroso
GB
Departamento de Análises Clínicas e Toxicológicas da Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil. gbcjunior@hotmail.com
Sales
Valeria Soraya de Farias
VS
Cavalcanti e Silva
Dany Geraldo Kramer
DG
Lopes
Maria Cleide de Araújo
MC
Paiva
Aldair de Souza
Ade S
da Fonseca
Henrique Eduardo Macedo
HE
do Nascimento Júniors
Francisco Fernandes
FF
Fernandes
Maria Zélia
MZ
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood; D018956:CD5 Antigens / Q000097:blood*; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping; D007943:Leukemia, Hairy Cell / Q000097:blood / Q000175:diagnosis; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000175:diagnosis*; D018655:Lymphocyte Count; D016393:Lymphoma, B-Cell / Q000097:blood / Q000175:diagnosis*; D020522:Lymphoma, Mantle-Cell / Q000097:blood / Q000175:diagnosis; D008232:Lymphoproliferative Disorders / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged
D014408:Biomarkers, Tumor; D018956:CD5 Antigens