A gene-based radiation hybrid map of chicken microchromosome 14: comparison to human and alignment to the assembled chicken sequence.
We present a gene-based RH map of the chicken microchromosome GGA14, known to have synteny conservations with human chromosomal regions HSA16p13.3 and HSA17p11.2. Microsatellite markers from the genetic map were used to check the validity of the RH map and additional markers were developed from chicken EST data to yield comparative mapping data. A high rate of intra-chromosomal rearrangements was detected by comparison to the assembled human sequence. Finally, the alignment of the RH map to the assembled chicken sequence showed a small number of discordances, most of which involved the same region of the chromosome spanning between 40.5 and 75.9 cR(6000) on the RH map.
Morisson
Mireille
M
Laboratoire de génétique cellulaire, Institut national de la recherche agronomique, Castanet-Tolosan, France. mmorisso@toulouse.inra.fr
Leroux
Sophie
S
Jiguet-Jiglaire
Carine
C
Assaf
Sirine
S
Pitel
Frédérique
F
Lagarrigue
Sandrine
S
Bardes
Suzanne
S
Feve
Katia
K
Faraut
Thomas
T
Milan
Denis
D
Vignal
Alain
A
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics*; D019295:Computational Biology; D017931:DNA Primers; D020224:Expressed Sequence Tags; D023061:Gene Order; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D021101:Radiation Hybrid Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D005819:Genetic Markers