title: Fluorescent multivalent opsonophagocytic assay for measurement of functional antibodies to Streptococcus pneumoniae.
abstract: We developed fluorescent mono- and multivalent opsonophagocytic assays (fOPA and fmOPA, respectively) specific for seven Streptococcus pneumoniae serotypes (4, 6B, 9V, 14, 18C, 19F, and 23F). Bacterial survival was quantitated with alamar blue, a fluorescent metabolic indicator. Both fOPA and fmOPA allow for determination of viability endpoints for up to seven serotypes with high levels of agreement to the reference method. The fmOPA eliminates colony counting, reduces serum volume, and produces results in 1 day.
authors.affiliation: Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.
authors.forename: Kathryn T
authors.initials: KT
authors.lastname: Bieging
authors.forename: Gowrisankar
authors.initials: G
authors.lastname: Rajam
authors.forename: Patricia
authors.initials: P
authors.lastname: Holder
authors.forename: Ross
authors.initials: R
authors.lastname: Udoff
authors.forename: George M
authors.initials: GM
authors.lastname: Carlone
authors.forename: Sandra
authors.initials: S
authors.lastname: Romero-Steiner
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D015169:Colony Count, Microbial; D006801:Humans; D009895:Opsonin Proteins; D010078:Oxazines; D010587:Phagocytosis; D011008:Pneumococcal Infections / Q000175:diagnosis*; D012703:Serotyping / Q000379:methods; D013296:Streptococcus pneumoniae / Q000145:classification / Q000166:cytology / Q000276:immunology*; D014966:Xanthenes
chemical_list: D000907:Antibodies, Bacterial; D009895:Opsonin Proteins; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin