Dynamics and persistence of Mycoplasma hyopneumoniae infection in pigs.
The purpose of this study was to describe the dynamics (shedding and transmission) of Mycoplasma hyopneumoniae infection within a population of swine and to determine the duration of the infection (persistence) through the identification of the agent in bronchial samples. Sixty-three 2-month-old pigs were used in this study. The pigs (n = 28) were experimentally infected by the intratracheal route with M. hyopneumoniae and considered as seeder pigs. The remaining pigs (n = 32) were not inoculated and randomly allocated to 2 different groups: direct contact exposure pigs (n = 12) and indirect contact exposure pigs (n = 20). Blood samples and nasal swabs were collected throughout the study on days 0, 28, 35, 42, 49, 63, 91, and 119 postinfection. To assess the duration of M. hyopneumoniae infection, 9 seeder and 6 contact exposure pigs were slaughtered at days 155 (group 1), 170 (group 2), and 185 (group 3) postinfection. Direct contact pigs showed evidence of infection on day 28 by polymerase chain reaction (PCR) and on day 35 by serology. The indirect contact exposure pigs presented a very delayed and slow seroconversion pattern; they did not present evidence of transmission until 42 d after the infection of seeder pigs. Identification of M. hyopneumoniae in bronchial swabs was confirmed by nested-PCR from days 155 to 185 postinfection. At the last slaughter date, 77.7% and 100% of the seeders and contact exposure pigs, respectively, tested positive. The results of this study reconfirmed direct infection of M. hyopneumoniae and suggest that indirect transmission can occur in a population. Finally, duration of the infection in this study was longer than previously described.
Fano
Eduardo
E
Swine Disease Eradication Center, 385C Animal Science/Veterinary Medicine Building, University of Minnesota College of Veterinary Medicine, St. Paul, Minnesota 55108, USA.
Pijoan
Carlos
C
Dee
Scott
S
D000818:Animals; D018562:Disease Transmission, Infectious / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008168:Lung / Q000382:microbiology* / Q000473:pathology; D045705:Mycoplasma hyopneumoniae / Q000254:growth & development / Q000302:isolation & purification; D045729:Pneumonia of Swine, Mycoplasmal / Q000097:blood / Q000453:epidemiology / Q000635:transmission*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011897:Random Allocation; D013552:Swine; D013997:Time Factors