Chemical synthesis of 23 kDa glycoprotein by repetitive segment condensation: a synthesis of MUC2 basal motif carrying multiple O-GalNAc moieties.
Peptide thioester corresponding to a MUC2 tandem repeat unit, which retains seven GalNAc moieties, was prepared by the Fmoc method followed by the low TfOH treatment to remove benzyl groups at the carbohydrate portions. The glycosylated peptide thioester was then consecutively joined by the activation of a thioester group by silver ions to obtain a MUC2 tandem repeat model composed of 141 amino acids with 42 GalNAc moieties.
Hojo
Hironobu
H
Department of Applied Biochemistry, Institute of Glycotechnology, Tokai University, Kitakaname 1117, Hiratsuka, Kanagawa 259-1292, Japan. hojo@keyaki.cc.u-tokai.ac.jp
Matsumoto
Yoshiyuki
Y
Nakahara
Yuko
Y
Ito
Emi
E
Suzuki
Yusuke
Y
Suzuki
Minoru
M
Suzuki
Akemi
A
Nakahara
Yoshiaki
Y
D000116:Acetylgalactosamine / Q000737:chemistry*; D000595:Amino Acid Sequence; D006023:Glycoproteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D055262:Mucin-2; D009077:Mucins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D006023:Glycoproteins; D055262:Mucin-2; D009077:Mucins; D000116:Acetylgalactosamine