title: Linkage map of protein-protein interactions of Porcine teschovirus.
abstract: A yeast two-hybrid study was conducted to catalogue the protein-protein interactions of the Porcine teschovirus non-structural proteins. Five homodimer, three reciprocal heterodimer and four unidirectional heterodimer interactions were observed. While several interactions are similar to those described in previous studies using enteroviruses, such as homo- and heterodimeric interactions of the 2B, 3CD and 3D proteins, several were not found previously. Among these is the binding of the leader protein L to the proteinases 3C and 3CD. Unlike the poliovirus 3C, the teschovirus 3C proteinase dimerizes and interacts with 2BC, 3CD and 3D. The strongest interactions were observed for L-3C, L-3CD and 3C-3CD.
authors.affiliation: Institute for Virology and Antiviral Therapy, Hans-Knöll-Str. 2, D-07745 Jena, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Zell
authors.affiliation: Institute for Virology and Antiviral Therapy, Hans-Knöll-Str. 2, D-07745 Jena, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Seitz
authors.affiliation: Institute for Virology and Antiviral Therapy, Hans-Knöll-Str. 2, D-07745 Jena, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Henke
authors.affiliation: Leibniz Institute for Natural Products Research and Infection Biology, Hans Knöll Institute, Beutenbergstr. 11a, D-07745 Jena, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Munder
authors.affiliation: Institute for Virology and Antiviral Therapy, Hans-Knöll-Str. 2, D-07745 Jena, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Wutzler
mesh_terms: D000086042:3C Viral Proteases; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D008040:Genetic Linkage; D010849:Picornaviridae / Q000235:genetics* / Q000502:physiology; D020798:Two-Hybrid System Techniques; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins
chemical_list: D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases