title: Biocompatible in-tube solid-phase microextraction coupled to HPLC for the determination of angiotensin II receptor antagonists in human plasma and urine.
abstract: A poly (methacrylic acid-ethylene glycol dimethacrylate, MAA-EGDMA) monolithic capillary was used for the in-tube solid-phase microextraction (in-tube SPME) of several angiotensin II receptor antagonists (ARA-IIs) from human plasma and urine. Under the optimized extraction condition, the protein component of the biological sample was flushed through the monolithic capillary, while the analytes were successfully trapped. Coupled to HPLC with fluorescence detection, this on-line in-tube SPME method was successfully applied for the determination of candesartan, losartan, irbesartan, valsartan, telmisartan, and their detection limits were found to be 0.1-15.3ng/mL and 0.1-15.2ng/mL in human plasma and urine, respectively. The method was linear over the range of 0.5-200ng/mL for telmisartan, 5-2000ng/mL for candesartan and irbesartan, 10-2000ng/mL for valsartan, and 50-5000ng/mL for losartan with correlation coefficients being above 0.9985 in plasma sample and above 0.9994 in urine sample. The method reproducibility was evaluated at three concentration levels, resulting in the R.S.D. <7%. The poly (MAA-EGDMA) monolithic capillary was demonstrated to be robust and biocompatible by using direct injections of biological samples.
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan 430072, PR China; Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Nie
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Fan
authors.forename: Yi
authors.initials: Y
authors.lastname: Wen
authors.forename: Bingren
authors.initials: B
authors.lastname: Xiang
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
mesh_terms: D057911:Angiotensin Receptor Antagonists; D001672:Biocompatible Materials / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D057911:Angiotensin Receptor Antagonists; D001672:Biocompatible Materials; D011108:Polymers; D011109:Polymethacrylic Acids; C099679:poly(methacrylic acid-ethylene glycol); D011092:Polyethylene Glycols