title: Streptococcus rattus strain BHT produces both a class I two-component lantibiotic and a class II bacteriocin.
abstract: Streptococcus rattus strain BHT is a species representative and strong bacteriocin producer. Here we report that S. rattus BHT produces two quite different types of bacteriocin activity, named BHT-A and BHT-B. The two bacteriocins were purified and analysed for activity and by MALDI-TOF mass spectrophotometry. BHT-A was found to be a variant of the two-component lantibiotic, Smb. BHT-B is a non-modified 5195Da peptide with some similarity to the tryptophan-rich Staphylococcus aureus bacteriocin, aureocin A53. Six S. rattus and two S. mutans strains were found to contain both the BHT-A and BHT-B genetic loci.
authors.affiliation: Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
authors.forename: Otto
authors.initials: O
authors.lastname: Hyink
authors.forename: Mayooran
authors.initials: M
authors.lastname: Balakrishnan
authors.forename: John R
authors.initials: JR
authors.lastname: Tagg
mesh_terms: D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000633:toxicity; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000633:toxicity*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013291:Streptococcus / Q000378:metabolism*
chemical_list: D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; D001430:Bacteriocins; D004269:DNA, Bacterial; D010455:Peptides; C498056:Smb lantibiotic; C469333:aureocin A53, Staphylococcus aureus