The in vitro effect of estradiol and testosterone on iron-induced lipid peroxidation in rat brain and kidney tissues.
The effects of estradiol and testosterone on iron-induced lipid peroxidation were compared in rat brain and kidney homogenates in vitro. Lipid peroxidation in the form of malondialdehyde (MDA) was evaluated by the measurement of thiobarbituric acid (TBA) reactive substances. Estradiol inhibited lipid peroxidation in both tissues studied in a dose-dependent manner. The effect was five times stronger in brain than in kidney. Testosterone did not affect lipid peroxidation in either tissue. Estradiol-induced inhibition of brain lipid peroxidation might contribute to the neuroprotective effect of estrogens.
Requintina
Pura J
PJ
Melatonin Clinic and Pineal Research Laboratory, Department of Psychiatry, Tufts University School of Medicine, Boston, Massachusetts 02135, USA. requintina@cox.net
Oxenkrug
Gregory F
GF
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D013739:Testosterone / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D017392:Thiobarbituric Acid Reactive Substances; D013739:Testosterone; D004958:Estradiol; D008315:Malondialdehyde