title: Oligonucleotide ligation assay for detection of mutations associated with reverse transcriptase and protease inhibitor resistance in non-B subtypes and recombinant forms of human immunodeficiency virus type 1.
abstract: The oligonucleotide ligation assay is a genotypic assay for the detection of resistance-associated mutations to reverse transcriptase and protease inhibitors in human immunodeficiency virus type 1 subtype B. This assay has been modified and developed for non-B subtypes and recombinant strains and has been evaluated with sequencing, resulting in a more sensitive assay than sequencing for non-B subtypes.
authors.affiliation: Area de Patogenia Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Vega
authors.forename: Lucía
authors.initials: L
authors.lastname: Pérez-Alvárez
authors.forename: Elena
authors.initials: E
authors.lastname: Delgado
authors.forename: Mercedes
authors.initials: M
authors.lastname: Muñoz
authors.forename: Gema
authors.initials: G
authors.lastname: Casado
authors.forename: Rocío
authors.initials: R
authors.lastname: Carmona
authors.forename: María
authors.initials: M
authors.lastname: Sierra
authors.forename: Elena
authors.initials: E
authors.lastname: Vázquez de Parga
authors.forename: Milagros
authors.initials: M
authors.lastname: Pinilla
authors.forename: Valentina
authors.initials: V
authors.lastname: García
authors.forename: Leandro
authors.initials: L
authors.lastname: Medrano
authors.forename: Gerardo
authors.initials: G
authors.lastname: Contreras
authors.forename: Miguel
authors.initials: M
authors.lastname: Thomson
authors.forename: Rafael
authors.initials: R
authors.lastname: Nájera
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology*; D011088:DNA Ligases / Q000378:metabolism*; D024882:Drug Resistance, Viral / Q000235:genetics*; D015684:Gene Products, pol / Q000235:genetics; D015658:HIV Infections / Q000821:virology; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D015497:HIV-1 / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017354:Point Mutation; D012367:RNA, Viral / Q000097:blood; D011995:Recombination, Genetic; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D019380:Anti-HIV Agents; D015684:Gene Products, pol; D017320:HIV Protease Inhibitors; D009841:Oligonucleotides; D012367:RNA, Viral; D018894:Reverse Transcriptase Inhibitors; D011088:DNA Ligases