Neuroprotective effects of the cyanidin-3-O-beta-d-glucopyranoside isolated from mulberry fruit against cerebral ischemia.
This study investigated the neuroprotective effects of cyanidin-3-O-beta-d-glucopyranoside (C3G) from the mulberry fruits on neuronal cell damage. A 1% HCl-MeOH mulberry fruit extract was shown to have a cytoprotective effect on PC12 cells that had been exposed to hydrogen peroxide. The extract inhibited the cerebral ischemic damage caused by oxygen glucose deprivation (OGD) in PC12 cells. The neuroprotective effect of the mulberry fruit extract was further demonstrated in vivo using a mouse-brain-injury model with a transient middle cerebral artery occlusion (MCAO). C3G was isolated as a neuroprotective constituent from the mulberry fruit extract. Compared with the control group, C3G had neuroprotective effects on the PC12 cells exposed to hydrogen peroxide in vitro and on cerebral ischemic damage in vivo.
Kang
Tong Ho
TH
Graduate School of East-West Medical Science, Kyunghee University, Kyunggi 449-701, South Korea.
Hur
Jin Young
JY
Kim
Hyun Bok
HB
Ryu
Jong Hoon
JH
Kim
Sun Yeou
SY
D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage*; D002545:Brain Ischemia / Q000473:pathology* / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D031625:Morus / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D016716:PC12 Cells; D010936:Plant Extracts / Q000008:administration & dosage; D051381:Rats
D000872:Anthocyanins; D018696:Neuroprotective Agents; D010936:Plant Extracts; C462279:cyanidin-3-O-beta-glucopyranoside