title: [Gene mutation in the areas of PreC/C and BCP of HBV DNA].
abstract: OBJECTIVE
  To investigate the gene mutation in the areas of pre core/core (Pre C/C) and basic core promotor (BCP) of HBV DNA and its clinical significance.
  
  
  METHODS
  The nt 1 735-1 965 segment of HBV DNA was amplified with PCR in 54 cases with chronic hepatitis B and 10 cases with post-hepatitis cirrhosis. Then the PCR product was sequenced.
  
  
  RESULTS
  There were 168 site mutations in 48.5% (33/68) cases with hepatitis B. The first ten mutation sites were nt 1 764 (58.8%), 1 762 (48.5%), 1 799 (21.0%), 1 766 (14.7%), 1 896 (13.2%), 1 754 (8.8%), 1 899 (8.8%), 1 768 (7.4%), 1 814 (7.4%) and 1 913 (7.4%). Three rare mutations of nt 1907, 1 922 and 1 923 were also detected. The mutations of nt 1 896, 1 764 and 1 762 were found in 16.7%, 35.2% and 35.2% of chronic hepatitis, and in 30.0%, 60.0% and 60.0% respectively of post-hepatitis cirrhosis cases. There was statistical significance between the two groups (P < 0.01).
  
  
  CONCLUSIONS
  The mutations in the areas of Pre C/C and BCP of HBV DNA might possibly be associated with liver fibrosis. There are many mutation sites in HBV DNA and mutation occurs frequently, therefore gene sequencing is helpful to the design of gene chip and to clinical application.
authors.affiliation: The Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Cheng-long
authors.initials: CL
authors.lastname: Sun
authors.forename: Hui-min
authors.initials: HM
authors.lastname: Wang
authors.forename: Yue-guo
authors.initials: YG
authors.lastname: Wang
authors.forename: Dong-lei
authors.initials: DL
authors.lastname: Zhang
authors.forename: Yue-ping
authors.initials: YP
authors.lastname: Wu
authors.forename: Jian-long
authors.initials: JL
authors.lastname: Zhao
mesh_terms: D004279:DNA, Viral / Q000097:blood / Q000235:genetics*; D005787:Gene Frequency; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000097:blood / Q000473:pathology / Q000821:virology*; D006801:Humans; D009154:Mutation; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004279:DNA, Viral