Expressions and clinical significances of angiopoietin-1, -2 and Tie2 in human gastric cancer.
The roles of angiopoietins in gastric cancer progression are still not fully understood. In this study the expressions of angiopoietin-1 (Ang-1), -2 (Ang-2) were compared by immunohistochemistry in 53 gastric cancer and 23 normal gastric mucosa samples. Results revealed that Ang-2 expression was significantly increased in gastric cancer tissues (74%) and was correlated with higher TNM stage, lymph node metastasis as well as distance metastasis. The expression of Ang-1 was also elevated in cancerous tissues (66%) and significantly associated with differentiation degree. In addition, Ang-2 as well as its receptor Tie2 expressions were higher in 12 pairs of gastric cancer tissue samples than those in corresponding adjacent samples by Western blot, while Ang-1 expression showed great heterogeneity. Furthermore, the expressions of Ang-1 and Ang-2 were almost positive in eight gastric cancer cell lines. Among them, AGS expressed both Ang-2 and a relatively moderate amount of Ang-2(443), a novel splice form of Ang-2, while others showed only Ang-2 mRNA expression.
Wang
Jun
J
State Key Laboratory of Cancer Biology and Institute of Digestive Disease, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
Wu
Kaichun
K
Zhang
Dexin
D
Tang
Hongwei
H
Xie
Huahong
H
Hong
Liu
L
Pan
Yanglin
Y
Lan
Mei
M
Hu
Shengjuan
S
Ning
Xiaoxuan
X
Fan
Daiming
D
D042683:Angiopoietin-1 / Q000235:genetics / Q000378:metabolism*; D042702:Angiopoietin-2 / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D042787:Receptor, TIE-2 / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology
D042683:Angiopoietin-1; D042702:Angiopoietin-2; D012333:RNA, Messenger; D042787:Receptor, TIE-2