title: Isolation and analysis of partial cDNA sequence coding for superoxide dismutase in Wuchereria bancrofti.
abstract: Molecular characterization of Wuchereria bancrofti is essential to develop suitable anti-filarial drugs and vaccines. We describe here isolation, sequence analysis and cloning of a partial cDNA of an enzyme superoxide dismutase from this parasite. The immunoscreening of a lambda zap W. bancrofti microfilarial (Mf) cDNA library with microfilaremic sera had resulted in the isolation of several seroreactive clones including, WbSOD. This clone contained a 309 bp insert and showed significant nucleotide and deduced amino acid sequence homologies to the superoxide dismutases of other nematode parasites. The antioxidant property of this enzyme may have important contribution in the defense mechanism of the parasite against host immune response.
authors.affiliation: Department of Biochemistry and J B Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram 442 102, Wardha, Maharashtra, India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Dabir
authors.forename: Snehal
authors.initials: S
authors.lastname: Dabir
authors.forename: B V
authors.initials: BV
authors.lastname: Siva Prasad
authors.forename: M V R
authors.initials: MV
authors.lastname: Reddy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013482:Superoxide Dismutase / Q000235:genetics*; D014958:Wuchereria bancrofti / Q000201:enzymology* / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D013482:Superoxide Dismutase