[Direct bone morphogenetic protein 2 gene therapy for repairing segmental radial defect in rabbits].
OBJECTIVE
To study the effect of direct bone morphogenetic protein 2 (BMP-2) gene therapy mediated by adenovirus on repairing bone defect.


METHODS
The radial defect models were made on 60 rabbits, which were evenly divided into 4 groups randomly. The 4 groups were treated with different materials: group A, adenovirus carrying BMP-2 gene (Ad-BMP-2) plus bovine cancellous bone (BCB); group B, reconstructed BMP-2 plus BCB; group C, Ad-Lacz plus BCB; and group D, only BCB scaffolds. The X-ray, histological examination, biomechanics analysis, and immunohistochemical staining were made 4, 8, and 12 weeks after the operation.


RESULTS
Group A gained better effect in the volume of new bones, the anti-bending intensity of the healing bone, and the expression of BMP-2 than those of group B. The defect in group A was healed. No new bones were observed in group C and group D.


CONCLUSIONS
Direct BMP-2 gene therapy is easy to perform and has very strong osteo-induction ability. It is a good method to repair segmental bone defects.
Li
Jianjun
J
Department of Orthopaedics, the Second Hospital of China Medical University, Liaoning Shenyang, 110003, P. R. China. lijianjun71@yahoo.com.cn
Bai
Lunhao
L
Sun
Hongbin
H
Han
Dong
D
Gu
Jiaxiang
J
Wang
Huan
H
Duan
Jingzhu
J
Xu
Xinxiang
X
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics*; D001861:Bone Regeneration; D018786:Bone Substitutes; D016025:Bone Transplantation; D002417:Cattle; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D011817:Rabbits; D011884:Radius / Q000293:injuries* / Q000473:pathology / Q000601:surgery; D011897:Random Allocation; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes