title: Human herpesvirus 6 open reading frame U14 protein and cellular p53 interact with each other and are contained in the virion.
abstract: A mass spectroscopic analysis of proteins from human herpesvirus 6 (HHV-6)-infected cells showed that the HHV-6 U14 protein coimmunoprecipitated with the tumor suppressor p53. The binding of U14 to p53 was verified by coimmunoprecipitation experiments in both Molt-3 cells infected with HHV-6 and 293 cells cotransfected with U14 and p53 expression vectors. Indirect immunofluorescence assays (IFAs) showed that by 18 h postinfection (hpi) U14 localized to the dot-like structures observed in both the nucleus and cytoplasm where p53 was partly accumulated. Despite Northern blotting evidence that U14 follows late kinetics, the U14 protein was detected immediately after infection (at 3 hpi) by IFA. In addition, by Western blotting, U14 was detected at 0 hpi or in the presence of cycloheximide which completely abolished the expression of IE1 protein. In addition to U14, p53 was detected at 0 hpi although it was not detected in mock-infected cells. Furthermore, both U14 and p53 were clearly detected in the viral particles by Western blotting and immunoelectron microscopy, supporting the idea that U14 and p53 are incorporated into virions. Our study provides the first evidence of the incorporation of cellular p53 into viral particles and suggests that p53 may play an important role in viral infection.
authors.affiliation: Department of Microbiology, Osaka University Graduate School of Medicine C1, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Takemoto
authors.forename: Masato
authors.initials: M
authors.lastname: Koike
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Mori
authors.forename: Sayoko
authors.initials: S
authors.lastname: Yonemoto
authors.forename: Yumi
authors.initials: Y
authors.lastname: Sasamoto
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kondo
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Uchiyama
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamanishi
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D015654:Herpesvirus 6, Human / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D016366:Open Reading Frames; D011485:Protein Binding; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000378:metabolism*; D014771:Virion / Q000737:chemistry / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53; D015678:Viral Structural Proteins