title: Circovirus-infected geese studied by in situ hybridization.
abstract: It has now been established that circovirus infection is common in farmed geese, but little is known about the clinicopathological significance of such infections. Ten clinically diseased geese suspected of being infected by circovirus were studied by in situ hybridization using a goose circovirus DNA probe. Circovirus DNA was demonstrated in the bursa of Fabricius (BF), spleen, thymus, bone marrow, liver, kidney, lung and heart, indicating that infection can be multisystemic. In some birds, virus DNA was present in very large quantities, most notably in the BF, liver and small intestine. With the exception of BF and thymus, there were no histological findings that would have suggested the presence of such quantities of circovirus DNA. In view of the very large quantities of virus DNA labelling present in some tissues, and by analogy to porcine circovirus type 2 infection and psittacine beak and feather virus infections, which are known to cause severe disease, and which have similar virus distribution to that found in our geese, it seems probable that the circovirus was important in the disease manifestations shown by the infected geese.
authors.affiliation: Veterinary Sciences Division, Department of Agriculture and Rural Development for Northern Ireland, Stormont, Belfast, BT4 3SD, UK. joan.smyth@uconn.edu
authors.forename: Joan
authors.initials: J
authors.lastname: Smyth
authors.forename: D
authors.initials: D
authors.lastname: Soike
authors.forename: Deborah
authors.initials: D
authors.lastname: Moffett
authors.forename: J H
authors.initials: JH
authors.lastname: Weston
authors.forename: D
authors.initials: D
authors.lastname: Todd
mesh_terms: D000818:Animals; D002060:Bursa of Fabricius / Q000821:virology; D018173:Circoviridae Infections / Q000662:veterinary*; D017925:Circovirus / Q000302:isolation & purification*; D004279:DNA, Viral; D005777:Geese / Q000821:virology*; D017403:In Situ Hybridization / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000821:virology*; D012680:Sensitivity and Specificity; D013950:Thymus Gland / Q000821:virology; D014018:Tissue Distribution
chemical_list: D004279:DNA, Viral