[Significance and expression of cyclooxygenase-2 mRNA in peripheral blood monocytes in patients with acute coronary syndrome].
OBJECTIVE
To determine the expression of cyclooxygenase-2 (COX-2) mRNA in peripheral blood monocytes in patients with acute coronary syndrome (ACS), and to explore the effect of the expression of COX-2 mRNA in ACS.


METHODS
The expressions of COX-2 mRNA in peripheral blood monocytes from 18 normal subjects and 42 ACS patients were analyzed by reverse transcription polymerase chain reaction (RT-PCR), and the monocytes from patients were incubated with celecoxib in vitro. The concentrations of interleukin-6 (IL-6) and matrix metalloproteinase-9 (MMP-9) in supernates of monocytes were measured by enzyme-linked immunosorbent assays (ELISA).


RESULTS
The expression of COX-2 mRNA and the secrections of IL-6 and MMP-9 in peripheral blood monocytes in ACS patients significantly increased compared with those from normal controls [0.61 +/- 0.17 vs 0.11 +/- 0.09; (97.24 +/- 11.21) ng/L vs (22.15 +/- 6.30) ng/L; (41.20 +/- 8.41) g/L vs (11.76 +/- 4.23) g/L; all P < 0.05, respectively]. Celecoxib reduced IL-6 and MMP-9 secretion level of monocytes from ACS patients up to 48% and 50% respectively (all P < 0.05), in a concentration-dependent manner.


CONCLUSIONS
COX-2 in peripheral blood monocytes may play an important role in the acute coronary syndrome.
Deng
Ping
P
Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Zhao
Shui-ping
SP
Huang
Hong-guang
HG
Wu
Jie
J
Li
Jiang
J
Zhou
Hong-nian
HN
D000368:Aged; D000787:Angina Pectoris / Q000097:blood / Q000201:enzymology*; D003324:Coronary Artery Disease / Q000097:blood / Q000201:enzymology*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D015850:Interleukin-6; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D020780:Matrix Metalloproteinase 9