title: Comparison of rectoanal mucosal swab cultures and fecal cultures for determining prevalence of Escherichia coli O157:H7 in feedlot cattle.
abstract: We compared fecal samples with samples collected with rectoanal mucosa swabs (RAMS) to determine the prevalence of Escherichia coli O157 in feedlot cattle (n = 747). Escherichia coli O157 was detected in 9.5% of samples collected with RAMS and 4.7% of samples tested by fecal culture. Pulsed-field gel electrophoresis analysis of isolates suggested that the strains colonizing the rectoanal junction were the same as those from the feces. Mucosal swab sampling was more sensitive than fecal sampling for determining the prevalence of E. coli O157 in feedlot cattle.
authors.affiliation: Department of Diagnostic Medicine/Pathobiology, 305 Coles Hall, Kansas State University, Manhattan, KS 66506-5606, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Greenquist
authors.forename: J S
authors.initials: JS
authors.lastname: Drouillard
authors.forename: J M
authors.initials: JM
authors.lastname: Sargeant
authors.forename: B E
authors.initials: BE
authors.lastname: Depenbusch
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Shi
authors.forename: K F
authors.initials: KF
authors.lastname: Lechtenberg
authors.forename: T G
authors.initials: TG
authors.lastname: Nagaraja
mesh_terms: D001003:Anal Canal / Q000382:microbiology; D000821:Animal Feed / Q000382:microbiology*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D007413:Intestinal Mucosa / Q000382:microbiology; D008297:Male; D015995:Prevalence; D012007:Rectum / Q000382:microbiology; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*