[Effect of nano-hydroxyapatite collagen bone and marrow mesenchymal stem cell on treatment of rabbit osteonecrosis of the femoral head defect].
OBJECTIVE
To evaluate the effect of nano-hydroxyapatite collagen (nHAC) bone and marrow mesenchymal stem cells (MSCs) on the treatment of rabbit osteonecrosis of the femoral head (ONFH) defect.


METHODS
From June to October 2004, animal models of ONFH defect were established in 45 New Zealand rabbits. They were divided into 3 groups randomly: In group A, as the control group, defect was not filled with any implants; In group B with nHAC; In group C with nHAC+MSC. Imaging and histological observation were made 4, 8, 12 weeks after operation.


RESULTS
group C had a better osteogenesis ability than group B and group A. group B had a better osteogenesis ability than group A. Obvious new bones and osteogenesis were observed in group C 4 weeks after operation. The defect areas in group C were almost repaired 12 weeks after operation.


CONCLUSIONS
nHAC has a better effect of osteo-conduction and it is a superior material for repairing bone defect of ONFH and of great value in treating ONFH when compounded with MSCs.
Sun
Wei
W
Center for Osteonecrosis and Joint Preserving & Reconstruction, China-Japan Friendship Hospital, Beijing, 100029, P. R. China.
Li
Zirong
Z
Shi
Zhencai
Z
Zhang
Nianfei
N
Li
Yan
Y
Cui
Fuzhai
F
D000818:Animals; D016026:Bone Marrow Transplantation; D018786:Bone Substitutes; D002478:Cells, Cultured; D003094:Collagen; D004195:Disease Models, Animal; D017886:Durapatite; D005240:Feasibility Studies; D005260:Female; D005271:Femur Head Necrosis / Q000473:pathology / Q000601:surgery*; D048091:Guided Tissue Regeneration; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D011817:Rabbits; D011897:Random Allocation; D023822:Tissue Engineering / Q000379:methods
D018786:Bone Substitutes; C509317:nano-hydroxyapatite-collagen; D003094:Collagen; D017886:Durapatite