title: Biosynthesis of active Bacillus subtilis urease in the absence of known urease accessory proteins.
abstract: Bacillus subtilis contains urease structural genes but lacks the accessory genes typically required for GTP-dependent incorporation of nickel. Nevertheless, B. subtilis was shown to possess a functional urease, and the recombinant enzyme conferred low levels of nickel-dependent activity to Escherichia coli. Additional investigations of the system lead to the suggestion that B. subtilis may use unidentified accessory proteins for in vivo urease activation.
authors.affiliation: Cell and Molecular Biology Program, Michigan State University, East Lansing, Michigan 48824-4320, USA.
authors.forename: Jong Kyong
authors.initials: JK
authors.lastname: Kim
authors.forename: Scott B
authors.initials: SB
authors.lastname: Mulrooney
authors.forename: Robert P
authors.initials: RP
authors.lastname: Hausinger
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism; D004789:Enzyme Activation; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D006160:Guanosine Triphosphate / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D007709:Klebsiella / Q000201:enzymology / Q000235:genetics; D009532:Nickel / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014510:Urease / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D009532:Nickel; D006160:Guanosine Triphosphate; D006867:Hydrolases; D014510:Urease