[Study on the expression of human acute myeloid leukemia retrovirus gene].
OBJECTIVE
To explore the potentiality of retroviral etiology in human acute myeloid leukemia (AML).


METHODS
The expression of retrovirus clone 6 # 11 in leukemic cells from 19 AML patients and peripheral blood mononuclear cells (PBMNCs) from 20 controls was studied by Northern blot and reverse transcriptase polymerase chain reaction (RT-PCR).


RESULTS
Northern blot revealed the positive bands of 9.4 kb and 4.5 kb in 18 of 19 AML patients, and RT-PCR a 790 bp product in 11 of 14 AML patients.


CONCLUSIONS
There was a specific expression of acute myeloid leukemia associated retrovirus in AML patients.
Xu
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X
Department of Hematology, The Second Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, China.
Xu
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Wang
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D000293:Adolescent; D000328:Adult; D015152:Blotting, Northern; D005260:Female; D006678:HIV / Q000235:genetics; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000209:etiology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D012190:Retroviridae / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction