A Lactobacillus acidophilus strain of human gastrointestinal microbiota origin elicits killing of enterovirulent Salmonella enterica Serovar Typhimurium by triggering lethal bacterial membrane damage.
The human gastrointestinal microbiota produces antagonistic activities against gastrointestinal bacterial pathogens. We undertook a study to investigate the mechanism(s) by which a Lactobacillus acidophilus strain of human microbiota origin antagonizes the gram-negative enteroinvasive pathogen Salmonella enterica serovar Typhimurium. We showed that the cell-free culture supernatant of L. acidophilus strain LB (LB-CFCS) induced the following effects in S. enterica SL1344: (i) a decrease in intracellular ATP that paralleled bacterial death, (ii) the release of lipopolysaccharide, (iii) permeabilization of the bacterial membrane, and (iv) an increase in the sensitivity of Salmonella to the lytic action of sodium dodecyl sulfate. Finally, we showed using two mutant strains of Salmonella, PhoP MS7953s and PmrA JKS1170, that the two-component regulatory systems PhoP-PhoQ and PmrA-PmrB that regulate the mechanisms of resistance to antibacterial agents in Salmonella did not influence the anti-Salmonella effect of LB-CFCS.
Coconnier-Polter
Marie-Hélène
MH
Faculté de Pharmacie, Unité Inserm 510, F-92296 Chātenay-Malabry, France.
Liévin-Le Moal
Vanessa
V
Servin
Alain L
AL
D000255:Adenosine Triphosphate / Q000378:metabolism; D000898:Antibiosis; D001433:Bacteriolysis; D002462:Cell Membrane / Q000187:drug effects* / Q000473:pathology; D002463:Cell Membrane Permeability / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D041981:Gastrointestinal Tract / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007779:Lactobacillus acidophilus / Q000254:growth & development* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D012486:Salmonella typhimurium / Q000254:growth & development* / Q000472:pathogenicity; D015398:Signal Transduction
D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; D000255:Adenosine Triphosphate