[Influence of regenerated fluid in vitreous cavity at various periods after vitreoretinal microsurgery on the proliferation and bFGF secretion of cultured human retinal pigment epithelial cells].
OBJECTIVE
To demonstrate the mechanism of positive effects of the sequential air-fluid exchange on the use of complicated vitroretinal microsurgery.


METHODS
Seventy-one patients who underwent vitreoretinal microsurgery were performed 2 or 3 times sequential air-fluid exchange. The regenerated fliud in vitreous cavity at various periods was collected to act on cultured human retinal pigment epithelial (RPE) cells,and then the secretion of bFGF and the expressions of bcl-2 and ki-67 by RPE cells were observed.


RESULTS
The expressions of bcl-2 and ki-67 were up-regulated and the secretion of bFGF significantly increased after RPE cells was acted with the regenerated fluid in the vitreous cavity.


CONCLUSIONS
The sequential air-fluid exchange can mechanically reduce intraocular growth factors after the vitreoretinal microsurgery, indirectly restrain the proliferation of RPE cell, and improve the successful rate of vitreoretinal microsurgery.
Zhou
Qing-hua
QH
Department of Ophthalmology, Second Xiangya Hospital, Central South University, Changsha 410011, China. zhqh1975@yahoo.com.cn
Zhu
Xiao-hua
XH
D002455:Cell Division; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis / Q000235:genetics; D008866:Microsurgery; D010857:Pigment Epithelium of Eye / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D012038:Regeneration; D012160:Retina / Q000601:surgery; D012163:Retinal Detachment / Q000517:prevention & control; D055502:Secondary Prevention; D014821:Vitrectomy; D018630:Vitreoretinopathy, Proliferative / Q000601:surgery*
D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D016222:Fibroblast Growth Factor 2