title: Analysis of methane monooxygenase genes in mono lake suggests that increased methane oxidation activity may correlate with a change in methanotroph community structure.
abstract: Mono Lake is an alkaline hypersaline lake that supports high methane oxidation rates. Retrieved pmoA sequences showed a broad diversity of aerobic methane oxidizers including the type I methanotrophs Methylobacter (the dominant genus), Methylomicrobium, and Methylothermus, and the type II methanotroph Methylocystis. Stratification of Mono Lake resulted in variation of aerobic methane oxidation rates with depth. Methanotroph diversity as determined by analysis of pmoA using new denaturing gradient gel electrophoresis primers suggested that variations in methane oxidation activity may correlate with changes in methanotroph community composition.
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England.
authors.forename: Ju-Ling
authors.initials: JL
authors.lastname: Lin
authors.forename: Samantha B
authors.initials: SB
authors.lastname: Joye
authors.forename: Johannes C M
authors.initials: JC
authors.lastname: Scholten
authors.forename: Hendrik
authors.initials: H
authors.lastname: Schäfer
authors.forename: Ian R
authors.initials: IR
authors.lastname: McDonald
authors.forename: J Colin
authors.initials: JC
authors.lastname: Murrell
mesh_terms: D000332:Aerobiosis; D017753:Ecosystem; D004586:Electrophoresis / Q000379:methods; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000378:metabolism*; D008772:Methylococcaceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D042083:Methylocystaceae / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride
chemical_list: D012965:Sodium Chloride; D010105:Oxygenases; C021555:methane monooxygenase; D008697:Methane