[Radiosensitivity on colorectal neoplasms by recombinant adenoviral-mediated wild-type p53 gene].
OBJECTIVE
To study the radiosensitization on the cells of colorectal cancer transfected with recombinant adenovirus vector-mediated wild-type p53.


METHODS
SW480 cells transfected by wild-type p53 were treated with 4 Gy and 6 Gy radiation. The expression of recombinant adenovirus vector-mediated wild-type p53 gene was detected by Western blotting. The inhibition rate of SW480 cells was examined by MTT, apoptotic rate by TdT-mediated dUTP nick end labeling (TUNEL) and proliferating cell nuclear antigen (PCNA) by immunohistochemical method.


RESULTS
SW480 cells transfected by wild-type p53 were inhibited significantly by 4 Gy and 6 Gy radiation. The level of apoptosis increased and the expression of PCNA decreased.


CONCLUSIONS
Cells of colorectal carcinoma transfected with wild-type p53 increases their radiation sensitivity.
Cui
Bin-bin
BB
Department of Abdominal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin 150040, China. 0451cbb@163.com
Liu
Ming
M
Zhao
Peng
P
Zhao
Jia-hong
JH
Dong
Xin-shu
XS
D000256:Adenoviridae / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology / Q000532:radiotherapy*; D016158:Genes, p53 / Q000528:radiation effects*; D005822:Genetic Vectors; D006801:Humans; D009154:Mutation; D011836:Radiation Tolerance; D014162:Transfection; D014407:Tumor Cells, Cultured