title: Macrolide resistance mediated by a Bifidobacterium breve membrane protein.
abstract: A gene coding for a hypothetical membrane protein from Bifidobacterium breve was expressed in Lactococcus lactis. Immunoblotting demonstrated that this protein is located in the membrane. Phenotypical changes in sensitivity towards 21 antibiotics were determined. The membrane protein-expressing cells showed higher levels of resistance to several macrolides.
authors.affiliation: Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (CSIC), Ctra. Infiesto s/n, Villaviciosa, Spain. amargolles@ipla.csic.es
authors.forename: Abelardo
authors.initials: A
authors.lastname: Margolles
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Moreno
authors.forename: Douwe
authors.initials: D
authors.lastname: van Sinderen
authors.forename: Clara G
authors.initials: CG
authors.lastname: de Los Reyes-Gavilán
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001644:Bifidobacterium / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D013294:Lactococcus lactis / Q000235:genetics; D018942:Macrolides / Q000494:pharmacology*; D008565:Membrane Proteins / Q000378:metabolism*; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; D008565:Membrane Proteins