Regulation of serine racemase activity by D-serine and nitric oxide in human glioblastoma cells.
In the human glioblastoma cell line U87, the activity of serine racemase (SR), catalyzing the isomerisation of serine, was inversely regulated by D-serine and nitric oxide (NO), a neuromodulator and a neurotransmitter, respectively. SR activity was dose-dependently enhanced up to five times in cells treated with 10 mM D-serine, whereas it was inhibited by NO. Furthermore, D-serine was found to induce the denitrosylation of SR purified from mouse brain. These results suggest that serine racemase activity in astrocyte is regulated inversely by d-serine and NO. SR should be inhibited through nitrosylation by NO and activated through denitrosylation elicited by D-serine.
Shoji
Kazuo
K
Biochemistry Section, Department of Neuroscience and Vision, University of Verona, Italy.
Mariotto
Sofia
S
Ciampa
Anna Rosa
AR
Suzuki
Hisanori
H
D000704:Analysis of Variance; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005909:Glioblastoma / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D009569:Nitric Oxide / Q000494:pharmacology*; D019998:Racemases and Epimerases / Q000378:metabolism*; D012694:Serine / Q000494:pharmacology*; D013997:Time Factors; D000099300:Serine Racemase
D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D019998:Racemases and Epimerases; D012694:Serine; D000099300:Serine Racemase