[Effect of tamoxifen on plasma proteins in diabetes mellitus type 1].
OBJECTIVE
Considering that important scientific advances have been obtained through studies based on experimental Diabetes mellitus, and that tamoxifen action in humans remains unknown, the aim of the present work is to follow the modifications promoted by diabetes and tamoxifen in the electrophoretic profile of plasmatic proteins.


METHODS
It was used 27 Wistar female rats (180-250 body weight), randomicaly divided into five groups: C1 (n = 3, received vehicle), C2 (n = 3, no treatment), T (n =5, treated with tamoxifen, 0.3mg/Kg/day), D (n = 8, experimental diabetes by estreptozotocin, 45mg/Kg and DT (n = 8, diabetic treated with tamoxifen). The electrophoresis was accomplished in cellulose acetate. pH 8.6-8.8, TECNOW chamber, and the strains were stained by Ponceau S. The total proteins were determined by the Biuret method (Labtest). Proteinograms were obtained in densitometer BioSystems BTS-235.


RESULTS
Albumin decreased progressively in the groups T, D and DT; a1 fraction increased in groups T and DT; a2 fraction increased in groups T and D, including a synergic effect in group DT; a fraction increased in groups T and D; a fraction increased in groups T, D and DT.


CONCLUSIONS
The results indicate an acute phase resposta, with synergic effect of tamoxifen and diabetes, suggesting a probable hepatic lesion.
Silva
Teresa Cristina P
TC
Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, UFRN.
Mota
Saul B
SB
Almeida
Maria Margareth C
MM
Ferreira
Elaine Cristina S
EC
Ururahy
Marcela A G
MA
Bezerra
João Felipe
JF
Pereira
Ney M L
NM
Ramos
Ana M O
AM
Almeida
Maria das Graças
Md
Rezende
Adriana A
AA
D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D051381:Rats; D017208:Rats, Wistar; D013629:Tamoxifen / Q000494:pharmacology*
D001786:Blood Glucose; D001798:Blood Proteins; D004965:Estrogen Antagonists; D013629:Tamoxifen