Effects of intestinal trefoil, factor on colonic mucosa in experimental colitis of rats.
In order to investigate the protective effects of intestinal trefoil factor (ITF) on colonic mucosa in experimental colitis of rats, ITF was detected by RT-PCR and immunohistochemistry at different time points. Three days after colitis induction, rats were treated with either 0.9% saline solution or rhITF. Pathological changes and the expression of iNOS mRNA, NO, MDA and SOD were measured respectively. It was found that ITF was mainly located in goblet cells, significantly higher in model group than in normal group (P<0.05). rhITF could increase the iNOS mRNA expression and NO contents, and there was statistically significant difference between rhITF group and model group (P<0.05). rhITF also caused an increase of MDA and a decrease of SOD, but there was no significant difference between two groups. These results indicated that ITF has apparent therapeutic effects in ulcerative colitis, which may be associated with iNOS and NO.
Yang
Tian
T
Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Zou
Kaifang
K
Qian
Wei
W
D019342:Acetic Acid; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D003106:Colon / Q000378:metabolism; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics; D010455:Peptides / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics; D000071164:Trefoil Factor-2
D010455:Peptides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000071164:Trefoil Factor-2; D008315:Malondialdehyde; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D019342:Acetic Acid