[Experimental model of primary cultured metastatic melanoma by fine needle aspiration].
OBJECTIVE
Establish an experimental model of metastatic melanoma primary culture cells from fine-needle aspiration biopsies (FNAB).


METHODS
Two metastatic melanoma primary culture cells from (FNAB) have been developed from patients who had been submitted to excision of metastatic lesions and they were identified by immunohistochemical analyses using S-100 and MB-45 melanoma markers.


RESULTS
The culture diagnostic was confirmed by immunohistochemical. The technique of FNAB offers the advantage of providing a sequential analysis of the same tumor nodules throughout treatment, and is a minimally invasive procedure with almost no associated morbidity.


CONCLUSIONS
The establishment of metastatic melanoma primary culture from FNAB samples showed a viable technique.
Oliveira
Andrea Fernandes de
AF
Laboratório de Cultura de Células, Universidade Federal de São Paulo, Escola Paulista de Medicina (UNIFESP/EPM), São Paulo. drandreafernandes@terra.com.br
Gragnani
Alfredo
A
Oliveira Filho
Renato Santos
RS
Santos
Ivan Dunshee de Abranches Oliveira
ID
França
Silvana Gaiba de
SG
Enokihara
Mílvia Maria Simões e Silva
MM
Ferreira
Lydia Masako
LM
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D001707:Biopsy, Needle / Q000379:methods; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008545:Melanoma / Q000473:pathology* / Q000556:secondary; D009418:S100 Proteins / Q000032:analysis
D014408:Biomarkers, Tumor; D009418:S100 Proteins