title: Analytical and biological evaluation of high throughput screen actives using evaporative light scattering, chemiluminescent nitrogen detection, and accurate mass LC-MS-MS.
abstract: In this investigation the utility of evaporative light scattering detection (ELSD) combined with HPLC-MS was demonstrated as a key component of a bioassay-guided fractionation, or "biofractionation" technique, for the evaluation of high throughput screen actives. ELSD provided on-line analyte mass information that was critical for the classification of the samples. Chemiluminescent nitrogen detection (CLND) was also evaluated for sample concentration estimation for nitrogen-containing compounds, and accurate mass LC-MS-MS analysis was employed for rapid structural confirmation and elucidation of components previously identified as active via biofractionation.
authors.affiliation: Lilly Research Laboratories, A Division of Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN 46285, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Peake
authors.forename: D C
authors.initials: DC
authors.lastname: Duckworth
authors.forename: T J
authors.initials: TJ
authors.lastname: Perun
authors.forename: W L
authors.initials: WL
authors.lastname: Scott
authors.forename: P
authors.initials: P
authors.lastname: Kulanthaivel
authors.forename: M A
authors.initials: MA
authors.lastname: Strege
mesh_terms: D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008027:Light; D008163:Luminescent Measurements; D013058:Mass Spectrometry / Q000379:methods*; D009584:Nitrogen / Q000032:analysis*; D012542:Scattering, Radiation
chemical_list: D009584:Nitrogen