Detection of fusion genes in sarcomas using paraffin-embedded tissues.
Many sarcomas are characterized by specific recurrent chromosomal translocations resulting in gene fusions. The genes involved in almost all of these translocations have been cloned, greatly changing sarcoma diagnosis. At the biological level, these chromosomal translocations produce highly specific fusion genes that encode key molecules for tumor development. The clinical correlation between these translocation-derived genetic markers and discrete histopathological entities has been remarkable. Today, detection of fusion genes plays a crucial role in the diagnosis of sarcomas that harbor atypical clinical or pathological presentations. The focus of this brief review is the recent impact that cytogenetic and molecular detection of these translocations has had on sarcoma diagnosis using paraffin-embedded sections.
Yoshida
Keiichi
K
Department of Pathology, Tokyo Medical University, Tokyo, Japan.
Oikawa
Kosuke
K
Takanashi
Masakatsu
M
Kuroda
Masahiko
M
D000818:Animals; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D015514:Oncogene Proteins, Fusion / Q000302:isolation & purification*; D016612:Paraffin Embedding; D012334:RNA, Neoplasm / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012509:Sarcoma / Q000235:genetics*
D015514:Oncogene Proteins, Fusion; D012334:RNA, Neoplasm