title: [Influence of vascular cell co-cultured with osteoblast in vitro on ability of osteoblast].
abstract: OBJECTIVE; The purpose of this study was to observe the influence on proliferation of rabbit osteoblast (ROB) of rabbit vascular endothelial cell(RVEC) co-cultured with rabbit osteoblast (ROB).
  
  
  METHODS
  Rabbit osteoblasts and rabbit vascular endothelial cells in ratio of 1:0, 1:1, 2:1, 4:1 were selected to co-culture. The function of osteoblasts was assessed by alkaline phosphatase (ALP) activity and osteocalcin (OC) assay.
  
  
  RESULTS
  There was good compatibility when osteoblasts and vascular endothelial cells were co-cultured. The activity of ALP and OC in the group of ROB and RVEC co-cultured in ratio of 2:1 was higher than that of the other three groups.
  
  
  CONCLUSIONS
  In direct co-culture system in vitro, RVEC can improve activity of ROB.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, PLA General Hospital, Beijing 100853, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D018920:Coculture Techniques; D066298:In Vitro Techniques; D010006:Osteoblasts; D011817:Rabbits
chemical_list: D000469:Alkaline Phosphatase