title: Effect of recombinant human tissue inhibitor of matrix metalloproteinase-1 in mandibular distraction osteogenesis in rabbits: a computed tomographic study.
abstract: Matrix metalloproteinases (MMPs), together with their tissue inhibitors (TIMPs), are responsible for the controlled degradation of collagen in bone. We studied the long-term stability of the regenerated bone formed by distraction osteogenesis, and the effect of recombinant human TIMP-1 on the remodelling of bone formed by mandibular distraction osteogenesis in rabbits. Nine rabbits were subjected to distraction osteogenesis of the mandible and divided into three groups: a control group with no collagen implanted; a sham-control group with a collagen sheet implanted; and an experimental group with a collagen sheet impregnated with rhTIMP-1 implanted. Computed tomograms were taken at weeks 4, 12, and 24 after distraction, and micro-computed tomograms and histological examinations were made at week 24. There was no significant resorption of regenerated bone at the site of distraction in any group after 6 months of consolidation, suggesting that the regenerated bone formed by distraction osteogenesis is stable. We found no obvious influence of rhTIMP-1 in the collagen sheet on the bony regenerate after 6 months of consolidation.
authors.affiliation: Discipline of Oral and Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China. lkcheung@hkucc.hku.hk
authors.forename: Lim Kwong
authors.initials: LK
authors.lastname: Cheung
authors.forename: Li Wu
authors.initials: LW
authors.lastname: Zheng
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D016723:Bone Remodeling / Q000187:drug effects; D001862:Bone Resorption / Q000000981:diagnostic imaging / Q000473:pathology; D003094:Collagen; D004337:Drug Carriers; D006801:Humans; D008334:Mandible / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology / Q000601:surgery*; D008567:Membranes, Artificial; D019857:Osteogenesis, Distraction; D011480:Protease Inhibitors / Q000627:therapeutic use*; D011817:Rabbits; D011994:Recombinant Proteins; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000627:therapeutic use*; D014057:Tomography, X-Ray Computed
chemical_list: D004337:Drug Carriers; D008567:Membranes, Artificial; D011480:Protease Inhibitors; D011994:Recombinant Proteins; D019715:Tissue Inhibitor of Metalloproteinase-1; D003094:Collagen