title: [Studies on chemical constitutes of green alga Chaetomorpha basiretorsa and their bioactivity].
abstract: OBJECTIVE
  To study the chemical constituents of Chaetomorpha basiretorsa and screen for bioactive leading compounds.
  
  
  METHODS
  Compounds were isolated by normal phase silica gel and Sephadex LH-20 gel column chromatography, reverse phase MPLC and reverse phase HPLC. Their structures were elucidated by spectroscopic methods including MS, IR and 1D, 2D NMR. Cytotoxicity of the compounds was screened by using standard MTT method. The purified compounds' inhibition against proliferation of dog vascular smooth muscle cells was also screened by MTT assay.
  
  
  RESULTS
  Five compounds were isolated from C. basiretorsa and their structures were identified as euphol (I), loloilide (II), 4-cumylphenol (III), zeaxanthin (IV) and lactucaxanthin (V).
  
  
  CONCLUSIONS
  All these compounds were obtained from this genus for the first time. Compound (III), 4-cumylphenol, was a new nature product. All compounds were inactive (IC50 > 10 microg x mL(-1)) in cytotoxicity screening. In inhibition against proliferation of dog vascular smooth muscle cells test, the cell survival ratio to compound I was (0.32 +/- 0.056)% which indicate its potential anti-atherosclerotic bioactivity.
authors.affiliation: Department of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Da-Yong
authors.initials: DY
authors.lastname: Shi
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Han
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Yong-Chun
authors.initials: YC
authors.lastname: Yang
authors.forename: Jian-Gong
authors.initials: JG
authors.lastname: Shi
authors.forename: Xiao
authors.initials: X
authors.lastname: Fan
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000460:Chlorophyta / Q000737:chemistry*; D004285:Dogs; D006801:Humans; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D024341:Xanthophylls / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D065146:Zeaxanthins
chemical_list: D010636:Phenols; D014315:Triterpenes; D024341:Xanthophylls; D065146:Zeaxanthins; D007810:Lanosterol; C516284:4-cumylphenol; C062557:euphol