title: [Expression of p21WAF1, p27KIP1 and cyclin E in ameloblastoma].
abstract: OBJECTIVE
  To investigate the expression of cyclin E mRNA, p21(WAF1) mRNA and p27(KIP1) protein in human ameloblastoma (AB), and to explore the clinical and biological characteristics of AB.
  
  
  METHODS
  The expression of cyclin E mRNA, p21(WAF1) mRNA and p27(KIP1) protein in 54 cases of human AB were detected by in situ hybridization or immunohistochemistry (SP method).
  
  
  RESULTS
  The positive expression rate of cyclin E mRNA in the cytoplasm or cell nucleus of AB was 66.7% (36/54). The expression of cyclin E mRNA increased with AB recurrence and malignant transformation, and the difference of expression among primary AB, recurrent AB, and malignant AB, was statistically significant. The positive expression ratio of cyclin E mRNA in OKC was 50.0% (8/16). The p21(WAF1) mRNA expression in the cytoplasm or cell nucleus of AB decreased, and the positive ratio was 22.6% (12/54) in AB, 37.5% (6/16) in OKC, respectively. The p27(KIP1) protein expression in the cell nucleus of AB was positive in a small number of cases, and the positive rate was 16.7% (9/54) in AB, 6.3% (1/16) in OKC, respectively.
  
  
  CONCLUSIONS
  The genesis and invasion of AB is associated with the cell proliferation and differentiation, and regulated by the higher expression of cyclin E and the lower expression of p21(WAF1) and p27(KIP1).
authors.affiliation: Department of Pathology, College of Stomatology, China Medical University, Shenyang 110002, China. zhongming1234@tom.com
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Yan-bing
authors.initials: YB
authors.lastname: Gong
authors.forename: Jing-dong
authors.initials: JD
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000564:Ameloblastoma / Q000378:metabolism* / Q000473:pathology; D002648:Child; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism*; D050760:Cyclin-Dependent Kinase Inhibitor p27; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007573:Jaw Neoplasms / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: C475820:CCNE1 protein, human; C493956:CDKN1A protein, human; C436291:CDKN1B protein, human; D019927:Cyclin E; D050759:Cyclin-Dependent Kinase Inhibitor p21; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D012333:RNA, Messenger; D050760:Cyclin-Dependent Kinase Inhibitor p27