Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro.
In this study concentration and composition of non-esterified fatty acids (NEFA) in follicular fluid (FF) of high-yielding dairy cows were determined during the period of negative energy balance (NEB) early post partum. NEFA were then added during in vitro maturation at concentrations measured previously in FF to evaluate their effect on the oocyte's developmental competence. At 16 and 44 days post partum, FF of the dominant follicle and blood were collected from nine high-yielding dairy cows. Samples were analysed for NEFA concentration and composition. NEFA concentrations in FF (0.2-0.6 mmol/l) during NEB remained +/- 40% lower compared with serum (0.4-1.2 mmol/l). The NEFA composition differed significantly between serum and FF with oleic acid (OA), palmitic acid (PA) and stearic acid (SA) being the predominant fatty acids in FF. Based on these results, 5115 oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of 0.200 mmol/l OA, 0.133 mmol/l PA or 0.067 mmol/l SA dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in SOF medium. Addition of PA or SA during oocyte maturation had negative effects on maturation, fertilization and cleavage rate and blastocyst yield. More (late) apoptotic cumulus cells were observed in cumulus-oocyte complexes matured in the presence of SA or PA. Ethanol or OA had no effect. These in vitro results suggest that NEB may hamper fertility of high-yielding dairy cows through increased NEFA concentrations in FF affecting oocyte quality.
Leroy
J L M R
JL
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. jo.leroy@UGent.be
Vanholder
T
T
Mateusen
B
B
Christophe
A
A
Opsomer
G
G
de Kruif
A
A
Genicot
G
G
Van Soom
A
A
D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002417:Cattle / Q000378:metabolism*; D002478:Cells, Cultured; D002970:Cleavage Stage, Ovum / Q000378:metabolism; D016895:Culture Media, Serum-Free; D004734:Energy Metabolism; D000431:Ethanol; D005230:Fatty Acids, Nonesterified / Q000032:analysis* / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry* / Q000378:metabolism; D008893:Milk Ejection; D019301:Oleic Acid / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D009866:Oogenesis / Q000502:physiology*; D019308:Palmitic Acid / Q000494:pharmacology; D049590:Postpartum Period / Q000378:metabolism; D013229:Stearic Acids / Q000494:pharmacology
D016895:Culture Media, Serum-Free; D005230:Fatty Acids, Nonesterified; D013229:Stearic Acids; D019301:Oleic Acid; D019308:Palmitic Acid; D000431:Ethanol; C031183:stearic acid