Indirect sandwich ELISA for antigen detection of African swine fever virus: comparison of polyclonal and monoclonal antibodies.
Two indirect, sandwich ELISAs are described for use in African swine fever (ASF) diagnosis. One assay uses polyclonal serum raised in rabbits and guinea pigs against the cytoplasmic soluble ASF virus protein and the second, a combination of monoclonal antibody raised against the VP73 protein and rabbit polyclonal serum. Both assays have been shown to detect antigen of representative field strains of phylogenetically distinct groupings of ASF virus but the ELISA, which utilises polyclonal antisera was slightly more sensitive than that using the monoclonal antibody.
Hutchings
Geoffrey H
GH
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK. geoff.hutchings@bbsrc.ac.uk
Ferris
Nigel P
NP
D000357:African Swine Fever / Q000175:diagnosis*; D000358:African Swine Fever Virus / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006168:Guinea Pigs; D011817:Rabbits; D012680:Sensitivity and Specificity; D013552:Swine; D014764:Viral Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D014764:Viral Proteins