Cell-specific targeting of lipid-based carriers for ODN and DNA.
It is well recognized that there is an urgent need for non-toxic systemically applicable vectors for biologically active nucleotides to fully exploit the current potential of molecular medicine in gene therapy. Cell-specific targeting of non-viral lipid-based carriers for ODN and DNA is a prerequisite to attain the concentration of nucleic acids required for therapeutic efficacy in the target tissue. In this review we will address the most promising approaches to selective targeting of liposomal nucleic acid carriers in vivo. In addition, the routes of entry and intracellular processing of these carrier systems are discussed as well as physiological factors potentially interfering with the biological and/or therapeutic activity of their nucleotide pay-load.
Bartsch
Martin
M
Department of Cell Biology, Section Liposome Research, Groningen University Institute for Drug Exploration (GUIDE), Groningen, The Netherlands.
Weeke-Klimp
Alida H
AH
Meijer
Dirk K F
DK
Scherphof
Gerrit L
GL
Kamps
Jan A A M
JA
D021581:Active Transport, Cell Nucleus; D000906:Antibodies / Q000378:metabolism; D002412:Cations / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism
D000906:Antibodies; D002412:Cations; D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface; D004247:DNA