Magnetic resonance imaging of mouse spinal cord.
The feasibility of performing high-resolution in vivo MRI on mouse spinal cord (SC) at 9.4 T magnetic field strength is demonstrated. The MR properties of the cord tissue were measured and the characteristics of water diffusion in the SC were quantified. The data indicate that the differences in the proton density (PD) and transverse relaxation time between gray matter (GM) and white matter (WM) dominate the contrast seen on the mouse SC images at 9.4 T. However, on heavily T(2)-weighted images these differences result in a reversal of contrast. The diffusion of water in the cord is anisotropic, but the WM exhibits greater anisotropy and principal diffusivity than the GM. The quantitative data presented here should establish a standard for comparing similar measurements obtained from the SCs of genetically engineered mouse or mouse models of SC injury (SCI).
Bilgen
Mehmet
M
Hoglund Brain Imaging Center, Kansas City, Kansas 66160, USA. mbilgen@kumc.edu
Al-Hafez
Baraa
B
Berman
Nancy E J
NE
Festoff
Barry W
BW
D000465:Algorithms; D000818:Animals; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods* / Q000662:veterinary*; D005240:Feasibility Studies; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013116:Spinal Cord / Q000166:cytology*