Expression of immunoglobulin E-dependent histamine-releasing factor in idiopathic nephrotic syndrome of childhood.
Concanavarin-A (conA)-stimulated peripheral blood mononuclear cells (PBMNC) from patients with idiopathic nephrotic syndrome (INS) produce putative factors that increase vascular permeability. These factors are expressed in the nephrotic phase but are reduced in the convalescent phase. To identify the genes that are expressed only in the nephrotic phase, we performed cDNA subtraction using conA-stimulated PBMNC from three patients with INS. We isolated several gene transcripts in all three subtracted cDNA libraries. Among these genes, IgE-dependent histamine-releasing factor (HRF) was overexpressed in the nephrotic phase not only at the mRNA level but also at the protein level in another 10 patients with INS. Moreover, we found increased secretion of HRF from conA-stimulated PBMNC in the nephrotic phase. The results suggest that HRF is involved in the pathogenesis of idiopathic nephrotic syndrome.
Toyabe
S
S
Division of Paediatrics, Department of Homeostatic Regulation and Development, Niigata Graduate School of Medical and Dental Sciences, Niigata University Medical and Dental Hospital, Japan. toyabe@med.niigata-u.ac.jp
Kaneko
U
U
Hara
M
M
Uchiyama
M
M
D014408:Biomarkers, Tumor; D002478:Cells, Cultured; D002648:Child; D003208:Concanavalin A / Q000276:immunology; D004270:DNA, Circular / Q000032:analysis; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D009363:Neoplasm Proteins / Q000276:immunology*; D009402:Nephrosis, Lipoid / Q000235:genetics / Q000276:immunology*; D009687:Nuclear Proteins / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000276:immunology; D014158:Transcription, Genetic / Q000235:genetics / Q000276:immunology; D000090324:Tumor Protein, Translationally-Controlled 1; D042461:Vascular Endothelial Growth Factor A / Q000276:immunology
D014408:Biomarkers, Tumor; D004270:DNA, Circular; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000090324:Tumor Protein, Translationally-Controlled 1; D042461:Vascular Endothelial Growth Factor A; D003208:Concanavalin A; D007073:Immunoglobulin E