title: Bacteriophages of freshwater Brevundimonas vesicularis isolates.
abstract: Nine strains of Brevundimonas vesicularis were isolated from surface water of three ponds in Bielefeld, Germany. With those strains as indicators seven bacteriophages with different host ranges were isolated. Molecular characterization showed that all phages contained linear double-stranded DNA with a similar genome size of about 37 kb. Restriction analysis and hybridization of phage DNAs revealed that three of these phages are closely related to each other. These phages had morphologies typical of the family Siphoviridae. Their genomes contained cohesive ends. Four phages were classified into the family of Podoviridae. Restriction analysis of the DNAs of these phages did not reveal any similarities. The DNA of these phages were terminally redundant. All phages were unable to transduce plasmids or marker genes.
authors.affiliation: Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Germany.
authors.forename: Frauke
authors.initials: F
authors.lastname: Beilstein
authors.forename: Brigitte
authors.initials: B
authors.lastname: Dreiseikelmann
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D042063:Caulobacteraceae / Q000235:genetics / Q000821:virology*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology* / Q000821:virology*; D006868:Hydrolysis; D017902:Podoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017906:Siphoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral; D004247:DNA; D004262:DNA Restriction Enzymes