Molecular testing of multiple HIV-1 transmissions in a criminal case.
OBJECTIVE
To test the a priori hypothesis of HIV-1 transmission from one suspect to six recipients in a criminal case.


METHODS
Partial pol and/or env sequences were obtained for at least two samples of the suspect and the victims. Appropriate local controls were sampled based on epidemiological and subtype criteria. Phylogenetic testing was performed using different reconstruction methods.


RESULTS
Phylogenetic analyses consistently inferred a monophyletic cluster for the suspect and victim samples in both genome regions. This was highly supported by parametric and non-parametric bootstrapping techniques. Moreover, the controls most closely related to the suspect-victim cluster had a similar geographical origin to the suspect.


CONCLUSIONS
Taking into account the limitations on the conclusions that can be drawn from molecular investigations we could infer that our molecular data is consistent with a scenario of multiple HIV transmission between suspect and victims.
Lemey
Philippe
P
Rega Institute, KULeuven, B-3000 Leuven, Belgium. philippe.lemey@uz.kuleuven.ac.be
Van Dooren
Sonia
S
Van Laethem
Kristel
K
Schrooten
Yoeri
Y
Derdelinckx
Inge
I
Goubau
Patrick
P
Brun-Vézinet
Francoise
F
Vaira
Dolores
D
Vandamme
Anne-Mieke
AM
D001499:Bayes Theorem; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D015752:Genes, env / Q000235:genetics; D015751:Genes, pol / Q000235:genetics; D015658:HIV Infections / Q000635:transmission* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D006801:Humans; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011902:Rape
D004279:DNA, Viral