High-throughput sequencing of high copy number plasmids from bacterial cultures by heat lysis.
Ganguly
Tapan
T
DNA Sequencing facility, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA. gangulyt@mail.med.upenn.edu
Chen
Peiqin
P
Teetsel
Rebecca
R
Zhang
Lan Ping
LP
Papaioannou
Elias
E
Cianciarulo
Joseph
J
D018929:Cell Culture Techniques / Q000379:methods*; D002458:Cell Fractionation / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D018628:Gene Dosage; D006358:Hot Temperature; D010957:Plasmids / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
D004269:DNA, Bacterial