title: Effects of angiotensin II and C-type natriuretic peptide on the in situ perfused trunk preparation of the dogfish, Scyliorhinus canicula.
abstract: The renal roles of physiologically relevant doses of angiotensin II (Ang II) and C-type natriuretic peptide (CNP) were investigated in the dogfish, Scyliorhinus canicula, using an in situ perfused trunk preparation. Perfusion with 10(-9) M Ang II resulted in a glomerular antidiuresis and decreases in perfusate flow rate, transport maxima for glucose and the proportion of filtering glomeruli. In addition, the renal clearances and excretion of urea, sodium, and chloride were significantly reduced, whereas the relative clearances of these parameters remained unchanged. In contrast, perfusion of 10(-9) M CNP caused a glomerular diuresis, an increase in transport maxima for glucose, but no significant change in the proportion of filtering glomeruli. In addition, the renal clearances of urea, sodium, and chloride were significantly increased but there was no effect on the relative clearances of urea, sodium, or chloride. Perfusion with 10(-10) M Ang II or CNP had no significant renal effects. Our results suggest that these hormones act at the level of the glomeruli rather than at a tubular level.
authors.affiliation: School of Biology, Gatty Marine Laboratory, University of St Andrews, St Andrews, Fife KY16 8LB, Scotland, UK. aw23@st-and.ac.uk
authors.forename: Alan
authors.initials: A
authors.lastname: Wells
authors.forename: W Gary
authors.initials: WG
authors.lastname: Anderson
authors.forename: Jenna E
authors.initials: JE
authors.lastname: Cains
authors.forename: Martin W
authors.initials: MW
authors.lastname: Cooper
authors.forename: Neil
authors.initials: N
authors.lastname: Hazon
mesh_terms: D000804:Angiotensin II / Q000493:pharmacokinetics* / Q000652:urine; D000818:Animals; D050034:Antidiuretic Agents / Q000494:pharmacology* / Q000652:urine; D002712:Chlorides / Q000652:urine; D004232:Diuretics / Q000494:pharmacology* / Q000652:urine; D004284:Dogfish / Q000502:physiology* / Q000652:urine; D005260:Female; D005919:Glomerular Filtration Rate / Q000187:drug effects / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D007444:Inulin / Q000652:urine; D007678:Kidney Glomerulus / Q000187:drug effects / Q000502:physiology; D007684:Kidney Tubules / Q000187:drug effects / Q000502:physiology; D020098:Natriuretic Peptide, C-Type / Q000494:pharmacology* / Q000652:urine; D009399:Nephrons / Q000187:drug effects* / Q000502:physiology; D010477:Perfusion / Q000379:methods; D012964:Sodium / Q000652:urine; D014508:Urea / Q000652:urine
chemical_list: D050034:Antidiuretic Agents; D002712:Chlorides; D004232:Diuretics; D000804:Angiotensin II; D020098:Natriuretic Peptide, C-Type; D014508:Urea; D007444:Inulin; D012964:Sodium; D005947:Glucose