title: Increased apical targeting of renal ENaC subunits and decreased expression of 11betaHSD2 in HgCl2-induced nephrotic syndrome in rats.
abstract: Nephrotic syndrome is often accompanied by sodium retention and generalized edema. We hypothesize that dysregulation of the epithelial sodium channel (ENaC) and/or of sodium (co)transporters may be responsible for the increased sodium retention associated with HgCl(2)-induced nephropathy. In addition, we examined the hypothesis that the expression of type 2 11beta-hydroxysteroid dehydrogenase (11betaHSD2) is reduced, contributing to the enhanced mineralocorticoid activity. Membranous nephropathy was induced in Brown Norway rats by repeated injections of HgCl(2) (1 mg/kg sc), whereas the control group received only vehicle. After 13 days of treatment, the abundance of ENaC subunits, sodium (co)transporters, and 11betaHSD2 in the kidney was examined by immunoblotting and immunohistochemistry. HgCl(2) treatment induced marked proteinuria, hypoalbuminemia, decreased urinary sodium excretion, and ascites. The protein abundance of alpha-ENaC was increased in the cortex/outer stripe of outer medulla (OSOM) and inner stripe of the outer medulla (ISOM). The protein abundances of beta-ENaC and gamma-ENaC were decreased in the cortex/OSOM while increased in the ISOM. Immunoperoxidase microscopy demonstrated increased targeting of ENaC subunits to the apical plasma membrane in the distal convoluted tubule, connecting tubule, and cortical and medullary collecting duct segments. Moreover, 11betaHSD2 abundance was decreased in cortex/OSOM and ISOM. The protein abundances of type 3 Na/H exchanger (NHE3), Na-K-2Cl cotransporter (NKCC2), and thiazide-sensitive Na-Cl cotransporter (NCC) were decreased. Moreover, the abundance of the alpha-1 subunit of the Na-K-ATPase was decreased in the cortex/OSOM and ISOM but remained unchanged in the inner medulla. These results suggest that increased apical targeting of ENaC subunits combined with diminished abundance of 11betaHSD2 may contribute to sodium retention associated with HgCl(2)-induced nephrotic syndrome. The decreased abundance of NHE3, NKCC2, NCC, and Na-K-ATPase may play a compensatory role in promoting sodium excretion.
authors.affiliation: The Water and Salt Research Center, Bldg. 233/234, University of Aarhus, DK-8000 Aarhus C, Denmark.
authors.forename: Soo Wan
authors.initials: SW
authors.lastname: Kim
authors.forename: Sophie
authors.initials: S
authors.lastname: de Seigneux
authors.forename: Martin C
authors.initials: MC
authors.lastname: Sassen
authors.forename: JongUn
authors.initials: J
authors.lastname: Lee
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
authors.forename: Mark A
authors.initials: MA
authors.lastname: Knepper
authors.forename: Jørgen
authors.initials: J
authors.lastname: Frøkiaer
authors.forename: Søren
authors.initials: S
authors.lastname: Nielsen
mesh_terms: D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2 / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D053503:Epithelial Sodium Channels; D005786:Gene Expression Regulation / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008627:Mercuric Chloride / Q000633:toxicity*; D008901:Mineralocorticoids / Q000378:metabolism; D009404:Nephrotic Syndrome / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology / Q000652:urine; D021122:Protein Subunits / Q000235:genetics / Q000502:physiology; D051381:Rats; D011914:Rats, Inbred BN; D012016:Reference Values; D012964:Sodium / Q000652:urine; D015222:Sodium Channels / Q000235:genetics / Q000502:physiology*
chemical_list: D053503:Epithelial Sodium Channels; D008901:Mineralocorticoids; D021122:Protein Subunits; D015222:Sodium Channels; D008627:Mercuric Chloride; D012964:Sodium; D043209:11-beta-Hydroxysteroid Dehydrogenase Type 2