[Effect of oral supplement of l-glutamine in colonic wall of rats subjected to abdominal irradiation].
OBJECTIVE
To evaluate the structural alterations of the irradiated colonic wall in rats, verifying if L-glutamine supplementation is able to prevent them.


METHODS
We used 30 male adult Wistar rats, divided into three groups: I--control, II--irradiated, and III-irradiated with L-glutamine supplementation during the 14 days of the study. Control group was maintained in laboratory standard conditions while groups II and three were submitted to abdominal radiation with an only dose of 1000 cGy in the 8th day of experimentation. All the animals were submitted to laparotomy in the 15th day for resection of the colonic segment for stereological analysis.


RESULTS
Group II presented total volume of colonic wall significantly smaller than control group without altering the partial volumes of each layer. Compared to groups II and III, group II exhibited maintenance of total volume of colonic wall, nearing control group. Compared to control group, animals of group III exhibited maintenance of epithelial partial volume without altering significantly epithelial surface.


CONCLUSIONS
It is suggested that L-glutamine supplementation can be of benefit in the irradiated colonic wall in rats.
Diestel
Cristina Fajardo
CF
Programa de Pós-Graduação em Ciências Médicas - FCM - UERJ. cdiestel@terra.com.br
Lopes-Paulo
Francisco
F
Marques
Ruy Garcia
RG
Horsts
Nara Limeira
NL
Caetano
Carlos Eduardo Rodrigues
CE
D034861:Abdominal Wall; D000284:Administration, Oral; D000818:Animals; D003106:Colon / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects*; D005973:Glutamine / Q000627:therapeutic use*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D008297:Male; D009929:Organ Size / Q000187:drug effects / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D013997:Time Factors
D005973:Glutamine