Presence of Borrelia burgdorferi sensu lato in mites parasitizing small rodents.
Ticks (especially those in the family Ixodidae) are the primary vectors of the infectious agent Borrelia burgdorferi sensu lato, which causes Lyme disease. To determine the potential role of mites as vectors of B. burgdorferi sensu lato, mites were collected from wild rodents in the Bazantula region of North Moravia (Czech Republic). These samples were examined for the presence of Borreliae by using DFM and PCR methods. Mites positive for the presence of DNA of B. burgdorferi sensu lato were determined as members of the families Haemogamasidae and Parasitidae. One sample from a mite of genus Haemogamasus was successfully isolated, and the specimen was confirmed as B. afzelii by using PCR-RFLP and by gradient SDS-PAGE. This suggests the possible participation of gamasid mites in borrelial circulation in nature and also points to the utility of further such studies to identify potential vectors (other than ticks) of the spirochete.
Netusil
J
J
Department of Comparative Animal Physiology and General Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic. 13446@mail.muni.cz
Zákovská
A
A
Horváth
R
R
Dendis
M
M
Janouskovcová
E
E
D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification*; D018153:Czech Republic; D004269:DNA, Bacterial / Q000032:analysis; D004199:Disease Vectors; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D008297:Male; D008924:Mite Infestations / Q000469:parasitology / Q000662:veterinary*; D008925:Mites / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D012376:Rodent Diseases / Q000469:parasitology*; D012377:Rodentia; D013045:Species Specificity
D004269:DNA, Bacterial