title: Revision of collagen molecular structure.
abstract: Based on the fiber diffraction data from native collagen, Rich and Crick proposed the 10/3-helical model with a 28.6 A axial repeat in 1955 (Rich A.; Crick, F. H. C. Nature (Lond) 1955, 176, 915-916). We obtained the 7/2-helical structure with a 20 A axial repeat from the single crystal analysis of (Pro-Pro-Gly)(10). Since the latter structure could explain fiber diffraction patterns from native collagen, we proposed this structure as a new model for collagen in 1977 (Okuyama et al., Polym J 1977, 9, 341-343). These two structural models were refined against observed continuous intensity data from native collagen using a linked-atom least-squares method. It was found that the diffraction data from native collagen could be explained by the 7/2-helical model better than, or at least the same as, the prevailing 10/3-helical model. Together with the evidence that recent single crystal analyses of many model peptides have supported the 7/2-helical model and there was no such active support for the 10/3-helical model, it was concluded that the average molecular structure of native collagen seems to be closer to the 7/2-helical symmetry than the other one.
authors.affiliation: Faculty of Technology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan. okuyamak@chem.sci.osaka-u.ac.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Okuyama
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Xu
authors.forename: Makoto
authors.initials: M
authors.lastname: Iguchi
authors.forename: Keiichi
authors.initials: K
authors.lastname: Noguchi
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification; D006860:Hydrogen Bonding; D007614:Macropodidae; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013710:Tendons / Q000737:chemistry; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D010455:Peptides; D014867:Water; D003094:Collagen