Monitoring Arthrobacter protophormiae RKJ100 in a 'tag and chase' method during p-nitrophenol bio-remediation in soil microcosms.
Monitoring of micro-organisms released deliberately into the environment is essential to assess their movement during the bio-remediation process. During the last few years, DNA-based genetic methods have emerged as the preferred method for such monitoring; however, their use is restricted in cases where organisms used for bio-remediation are not well characterized or where the public domain databases do not provide sufficient information regarding their sequence. For monitoring of such micro-organisms, alternate approaches have to be undertaken. In this study, we have specifically monitored a p-nitrophenol (PNP)-degrading organism, Arthrobacter protophormiae RKJ100, using molecular methods during PNP degradation in soil microcosm. Cells were tagged with a transposon-based foreign DNA sequence prior to their introduction into PNP-contaminated microcosms. Later, this artificially introduced DNA sequence was PCR-amplified to distinguish the bio-augmented organism from the indigenous microflora during PNP bio-remediation.
Pandey
Gunjan
G
Institute of Microbial Technology, Sector 39-A, Chandigarh, 160036, India.
Pandey
Janmejay
J
Jain
Rakesh K
RK
D001173:Arthrobacter / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods*; D009596:Nitrophenols / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009596:Nitrophenols; D012989:Soil Pollutants; C024836:4-nitrophenol