Development of efficient packaging method of oligodeoxynucleotides by a condensed nano particle in lipid envelope structure.
An efficient delivery system is required if antisense oligodeoxynucleotides (ODN) are to be utilized for gene therapy. We report herein on the development of a novel ODN delivery system, ODN-encapsulated nano particles (ODN-ENP) using an efficient and simple packaging method. The ODN-ENP consists of a condensed ODN particle and a lipid envelope, which can be equipped with various functional devices for the efficient delivery of ODN with a small diameter (150 nm). The encapsulation efficiency and ODN recovery of ODN-ENP were significantly higher than those of other packaging methods, such as a stabilized antisense-lipid particles method or a freeze-thaw method. Furthermore, the time required for the preparation of the ODN-ENP was shorter than the other methods. The method developed in this study is a simple and efficient packaging method for ODN with a condensed nano particle in lipid-envelope structure.
Yamada
Yuma
Y
Graduate School of Pharmaceutical Sciences, Hokkaido University; Kita-12, Nishi-6, Sapporo 060-0812, Japan.
Kogure
Kentaro
K
Nakamura
Yoshio
Y
Inoue
Kaori
K
Akita
Hidetaka
H
Nagatsugi
Fumi
F
Sasaki
Shigeki
S
Suhara
Tetsuya
T
Harashima
Hideyoshi
H
D001483:Base Sequence; D004337:Drug Carriers; D005615:Freezing; D008055:Lipids / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010316:Particle Size
D004337:Drug Carriers; D008055:Lipids; D009838:Oligodeoxyribonucleotides