title: Decreased tissue accumulation of 6-deoxy-6-[18F]fluoro-L-ascorbic acid in glutathione-deficient rats induced by administration of diethyl maleate.
abstract: The relationship between in vivo biodistribution of 6-deoxy-6-[18F]fluoro-L-ascorbic acid (18F-DFA) and the content of tissue glutathione (GSH) was investigated in Wistar male rats. Following intravenous administration of 18F-DFA, the accumulation of radioactivity in most tissues, including the adrenal glands, liver and brain, was significantly reduced together with a decrease in the content of GSH by preloading of diethyl maleate (DEM) which depletes cellular GSH. Similar decreased uptake was also observed in the distribution of L-[1-14C]ascorbic acid (14C-AA) after DEM treatment. The possible biological mechanisms, including competition with endogenous AA and ascorbate recycling, that modulate the uptake and accumulation into tissues of 18F-DFA and 14C-AA in GSH-deficient rats are discussed.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kyushu University; Fukuoka 812-8582, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Yamamoto
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kaneshiro
authors.forename: Hiroharu
authors.initials: H
authors.lastname: Kato
authors.forename: Takahiro
authors.initials: T
authors.lastname: Mukai
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kuwabara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Honda
authors.forename: Minoru
authors.initials: M
authors.lastname: Maeda
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D005978:Glutathione / Q000172:deficiency*; D008297:Male; D008298:Maleates / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D008298:Maleates; C076401:6-deoxy-6-fluoroascorbic acid; C014476:diethyl maleate; D005978:Glutathione; D001205:Ascorbic Acid