Dietary carotenoid pigment supplementation influences hepatic lipid and mucopolysaccharide levels in rainbow trout (Oncorhynchus mykiss).
We assessed the effects of dietary carotenoid pigment supplementation on liver histochemistry in the rainbow trout. One hundred and eight rainbow trout (mean mass 266+/-10 g) were assigned to each of three replicate tanks for each of three dietary treatments; astaxanthin, canthaxanthin, or control at a target dietary inclusion of 100 mg/kg, by top-coating a pigment-free commercially extruded basal diet (Trouw Aquaculture, U.K.). Fish were fed for 3 weeks at a ration of 1.2% body mass/day, in a recirculating freshwater system maintained at 16 degrees C. Frozen liver sections were stained for total lipids, unsaturated lipids, glycogen, mucopolysaccharides, glycogen phosphorylase and aspartate aminotransferase. Relative amounts were measured quantitatively by image analysis. Carotenoid treatment significantly (P<0.05) altered the total lipid profile and hepatic mucopolysaccharide contents of livers of rainbow trout. Results are discussed in relation to the catabolic potential of the liver in carotenoid pigment metabolism.
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Maple Leaf Foods Agresearch, 150 Research Lane, Suite 200, Guelph, Ontario, Canada N1G 4T2. pagegi@mapleleaf.ca
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Davies
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D000818:Animals; D016644:Canthaxanthin / Q000378:metabolism / Q000494:pharmacology; D002338:Carotenoids / Q000378:metabolism / Q000494:pharmacology*; D019587:Dietary Supplements / Q000009:adverse effects*; D006025:Glycosaminoglycans / Q000097:blood / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000097:blood / Q000378:metabolism*; D010860:Pigments, Biological / Q000378:metabolism / Q000494:pharmacology*; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology
D006025:Glycosaminoglycans; D008055:Lipids; D010860:Pigments, Biological; D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; D016644:Canthaxanthin; C005948:astaxanthine