title: Patient-based cross-platform comparison of oligonucleotide microarray expression profiles.
abstract: The comparison of gene expression measurements obtained with different technical approaches is of substantial interest in order to clarify whether inter-platform differences may conceal biologically significant information. To address this concern, we analyzed gene expression in a set of head and neck squamous cell carcinoma patients, using both spotted oligonucleotide microarrays made from a large collection of 70-mer probes and commercial arrays produced by in situ synthesis of sets of multiple 25-mer oligonucleotides per gene. Expression measurements were compared for 4425 genes represented on both platforms, which revealed strong correlations between the corresponding data sets. Of note, a global tendency towards smaller absolute ratios was observed when using the 70-mer probes. Real-time quantitative reverse transcription PCR measurements were conducted to verify expression ratios for a subset of genes and achieved good agreement regarding both array platforms. In conclusion, similar profiles of relative gene expression were obtained using arrays of either single 70-mer or multiple short 25-mer oligonucleotide probes per gene. Although qualitative assessments of the expression of individual genes have to be made with caution, our results indicate that the comparison of gene expression profiles generated on these platforms will help to discover disease-related gene signatures in general.
authors.affiliation: 1Division of Molecular Genetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Schlingemann
authors.forename: Negusse
authors.initials: N
authors.lastname: Habtemichael
authors.forename: Carina
authors.initials: C
authors.lastname: Ittrich
authors.forename: Grischa
authors.initials: G
authors.lastname: Toedt
authors.forename: Heidi
authors.initials: H
authors.lastname: Kramer
authors.forename: Markus
authors.initials: M
authors.lastname: Hambek
authors.forename: Rainald
authors.initials: R
authors.lastname: Knecht
authors.forename: Peter
authors.initials: P
authors.lastname: Lichter
authors.forename: Roland
authors.initials: R
authors.lastname: Stauber
authors.forename: Meinhard
authors.initials: M
authors.lastname: Hahn
mesh_terms: D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D017931:DNA Primers; D015342:DNA Probes; D005260:Female; D020869:Gene Expression Profiling; D006258:Head and Neck Neoplasms / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D017931:DNA Primers; D015342:DNA Probes