Improved detection of Mycobacterium paratuberculosis in milk.
At present there is no rapid microbiological method for the detection of viable Mycobacterium paratuberculosis in milk. By combining an extensive milk sample pretreatment with solid phase cytometry as the detection technique we were able to demonstrate viable mycobacterial cells in 50 ml of artificially contaminated pasteurized milk in less than one working day.
D'Haese
Eva
E
Scientific Institute of Public Health, Juliette Wytsmanstraat 14, B-1050 Brussels, Belgium.
Dumon
Iris
I
Werbrouck
Hadewig
H
De Jonghe
Valerie
V
Herman
Lieve
L
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification; D008892:Milk / Q000382:microbiology*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000302:isolation & purification*; D020102:Mycobacterium smegmatis / Q000302:isolation & purification; D012680:Sensitivity and Specificity