Analysis of the oscillatory kinetics of glycolytic intermediates in a yeast extract by FT-IR spectroscopy.
In the present work we demonstrate that FT-IR spectroscopy is a powerful tool for the time resolved and noninvasive measurement of multi-substrate/product interactions in complex metabolic networks as exemplified by the oscillating glycolysis in yeast extract. We found that many of the glycolytic intermediates can be identified with FT-IR spectroscopy. For this, we have constructed a spectral library of most of the glycolytic intermediates and obtained the kinetics of single components in spectra from glycolysing yeast extract by the use of mathematical fitting procedures. The results are in good agreement with the known phase relationships of oscillatory glycolysis. They provide the basis for future application of this method to investigate the energy metabolism of living cells.
Mair
Thomas
T
Otto-von-Guericke-Universitaet, Institute of Experimental Physics, Group of Biophysics, Universitaetsplatz 2, 39106 Magdeburg, Germany. thomas.mair@physik.uni-magdeburg.de
Zimányi
László
L
Khoroshyy
Petro
P
Müller
Andrea
A
Müller
Stefan C
SC
D000465:Algorithms; D001683:Biological Clocks / Q000502:physiology*; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D005656:Fungal Proteins / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000502:physiology*; D007700:Kinetics; D008954:Models, Biological; D012440:Saccharomyces / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D005656:Fungal Proteins; D005947:Glucose