title: Effect of a fluoride-releasing one-step adhesive system on monkey pulp tissue.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the pulpal response of a fluoride-releasing one-step adhesive in nonexposed monkey teeth.
  
  
  METHODS
  Cervical Class V cavities were prepared in monkey teeth. The cavities were divided into three groups of different restorative materials. A one-step adhesive (SI-IB551, Shofu) was applied to the teeth, and the cavities were filled with a resin composite (Beautifil, Shofu). In the other two groups, a two-step adhesive (Imperva FluoroBond, Shofu) together with either a resin composite (Beautifil) or a glass-ionomer cement (Fuji II, GC) was placed in the cavities. The teeth were then extracted after 3, 30, and 90 days, fixed in 10% buffered formalin solution, and prepared according to routine histological techniques. Five-micrometer sections were stained with hematoxylin and eosin, or Brown & Brenn gram stain for bacterial observation. Four histological features, odontoblastic change, inflammatory cell infiltrate, reparative dentin formation, and the bacterial staining, were evaluated and compared. The results were compared statistically with significance defined as p < 0.05 (Kruskall-Wallis test).
  
  
  RESULTS
  Disarrangement of the odontoblasts and slight inflammatory cell infiltrations were the main initial reactions, while deposition of reparative dentin was the major long-term reaction in all groups. No statistically significant differences in the four histological features were seen among the restorative materials placed in the cavities (p > 0.05). No serious pulpal inflammatory reactions, such as necrosis or abscess formation, were observed in any of the experimental groups.
  
  
  CONCLUSIONS
  The one-step adhesive showed acceptable biological compatibility with the monkey pulp, whereas the pulpal response to the system was minimally different from that of the glass-ionomer cement or the two-step self-etching adhesive.
authors.affiliation: Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. shimada.ope@tmd.ac.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shimada
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Seki
authors.forename: Mohammed Akhtar
authors.initials: MA
authors.lastname: Uzzaman
authors.forename: Richard M
authors.initials: RM
authors.lastname: Foxton
authors.forename: Junji
authors.initials: J
authors.lastname: Tagami
mesh_terms: D000818:Animals; D003763:Dental Leakage; D003782:Dental Pulp / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D005899:Glass Ionomer Cements / Q000494:pharmacology*; D000882:Haplorhini; D009804:Odontoblasts / Q000187:drug effects*
chemical_list: D017220:Dentin-Bonding Agents; D005899:Glass Ionomer Cements; C474103:imperva fluoro bond; C084541:Fuji glass-ionomer lining cement