title: Functional glucosylation of kojic acid and daidzein with the Eucalyptus membrane-associated UDP-glucosyltransferase reaction system.
abstract: Kojic acid was glucosylated to kojic acid 5- and 7-O-beta-D-glucopyranosides by cultured cells of Eucalyptus perriniana. The glucosylation was also performed effectively by the enzymatic reaction system using membrane-associated UDP-glucosyltransferase from the cells with addition of UDP-glucose. The production of the 5-O-D-glucoside occurred exclusively prior to that of the 7-O-glucoside in both systems. In the same manner, daidzein was converted to daidzin (daidzein 7-O-beta-D-glucopyranoside) by the enzymatic system. Here, we propose methods for the structural modification of phytoestrogen-related substances by enzymatic beta-glucosylation.
authors.affiliation: Graduate School of Health and Welfare Science, Okayama Prefectural University, Soja, Okayama 719-1197, Japan. nakajima@fhw.oka-pu.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Ishihara
authors.forename: H
authors.initials: H
authors.lastname: Hamada