DNA prime followed by protein boost enhances neutralization and Th1 type immunity against FMDV.
Prime-boost strategy has been exhibited its potency to enhance immune responses, which would be important to the success to develop a vaccine against the foot-and-mouth disease virus (FMDV). An eukaryotic expression construct encoding the FMDV capsid VP1 protein with a recombinant VP1 protein or a commercial FMDV vaccine were tested in the prime-boost strategy in mice and cattle trials. The levels of induced specific antibodies, T cell proliferations, and DTH activities were significantly higher in the prime-boost groups than in those vaccinated with DNA, protein or FMDV vaccine alone. More importantly, the levels of neutralizing antibodies in the former groups were significantly higher than others and could last for at least four months in cattle trials. This study suggests that the prime-boost strategy significantly improves the effective immunity and may provide a longer protection against FMDV infection.
Jin
Huali
H
State Key Laboratory for Agro-Biotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China.
Kang
Youmin
Y
Xiao
Chong
C
Zhu
Kaichun
K
Ma
Yijie
Y
Xie
Qifa
Q
Ma
Junwu
J
Xie
Qingge
Q
He
Cheng
C
Yang
Zhansheng
Z
Sun
Zhi
Z
Zhang
Xinyu
X
Chen
Mingyong
M
Zhang
Fuchun
F
Wang
Bin
B
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D036022:Capsid Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D016207:Cytokines / Q000235:genetics; D005260:Female; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D006968:Hypersensitivity, Delayed; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics
D000914:Antibodies, Viral; D036022:Capsid Proteins; D016207:Cytokines; D007074:Immunoglobulin G; D012333:RNA, Messenger; D011994:Recombinant Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; D019444:Vaccines, DNA; D014765:Viral Vaccines