title: [Expression specificity of hpt gene in sck transgenic rice].
abstract: OBJECTIVE
  Evaluate the expression specificity of hpt gene in different developmental stages of sck transgenic rice.
  
  
  METHODS
  The different tissues, including leaves, culms, roots and seeds (ripening stage), in different developmental stages of sck transgenic rice were detected by PCR. The positive plants and the control plants were detected by Northern blot analysis and double sandwich ELISA test.
  
  
  RESULTS
  The hpt gene expression was found at transcription level and translation level in different tissues of sck transgenic rice. HPT protein in the seeds could not be detected.
  
  
  CONCLUSIONS
  The hpt gene in different developmental stages of sck transgenic rice express stably at transcription level and translation level and HPT protein in the seeds could not be detected.
authors.affiliation: National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Song-Biao
authors.initials: SB
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhuo
authors.forename: Li-Chen
authors.initials: LC
authors.lastname: Yang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yang
mesh_terms: D018506:Gene Expression Regulation, Plant; D005819:Genetic Markers; D012275:Oryza / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D012639:Seeds / Q000378:metabolism
chemical_list: D005819:Genetic Markers; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase