Alterations in steroid hormone production by porcine ovarian granulosa cells caused by bisphenol A and bisphenol A dimethacrylate.
We have investigated the effects of bisphenol A (BPA) and BPA-dimethacrylate (BPA-DMA), endocrine disruptors used as plasticizers, on steroid hormone production by porcine ovarian granulosa cells after 72 h incubation. BPA at 10(-8) M to 10(-5) M increased basal progesterone levels, while the same concentration range of BPA-DMA did not cause any changes. After FSH-stimulation of the cells, BPA-DMA showed a tendency to inhibit progesterone production. BPA, however, at 10(-7) M and 10(-6) M concentrations was even able to amplify FSH-stimulated progesterone synthesis. BPA as well as BPA-DMA inhibited FSH-induced estradiol production in the whole concentration range. LH-stimulated progesterone production was not altered by BPA in 10(-8) M to 10(-5) M, while BPA-DMA decreased progesterone levels in the cultured media. Significant inhibitory effect of both tested agents at 10(-4) M concentrations was observed specifically on progesterone production, basal as well as gonadotropin-stimulated. The results indicate that ovarian steroidogenesis might be one of the possible sites afflicted by the endocrine disrupting action of BPA and BPA-DMA.
Mlynarcíková
Alzbeta
A
Institute of Experimental Endocrinology, SAS, Vlárska 3, 833 06 Bratislava, Slovakia.
Kolena
Jaroslav
J
Ficková
Mária
M
Scsuková
Sona
S
D000818:Animals; D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors / Q000494:pharmacology*; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D008689:Methacrylates / Q000494:pharmacology*; D010636:Phenols / Q000494:pharmacology*; D010968:Plasticizers / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis*; D013552:Swine; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004958:Estradiol; D008689:Methacrylates; D010636:Phenols; D010968:Plasticizers; D011374:Progesterone; C002462:4,4'-isopropylidenediphenol dimethacrylate; D000098826:Bisphenol A Compounds; C006780:bisphenol A