[Role of tissue factor in lung metastasis of colorectal carcinoma cells in vivo].
OBJECTIVE
To investigate the role of tissue factor(TF) in hematogenous metastasis of human colorectal carcinoma cells (LOVO) in vivo.


METHODS
The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFc DNA were transfected into LOVO cells by lipofectamine 2000. TF protein expression in the transfected cells was detected by Western blot. Eighteen nude mice (Babl/c nu/nu) were randomly divided into three groups, and then transfected and untransfected LOVO cells were implanted via tail vein respectively. The nude mice were sacrificed 8 weeks after implantation, and the number of metastatic nodules in the lung was used to assess the metastatic ability of LOVO cells.


RESULTS
Compared with the untransfected group, TF expression of LOVO cells and the numbers of metastatic nodules in the lung increased in sense-TF cDNA transfection group (P< 0.05, P< 0.01, respectively), whereas decreased in antisense-TF cDNA transfection group (P< 0.05, P< 0.01, respectively).


CONCLUSIONS
TF can increase the hematogenous metastatic ability of human colorectal carcinoma cells (LOVO) in vivo.
Rong
Long
L
Department of General Surgery, The First Hospital of Peking University, Beijing 100034, China. ronglong8@sina.com
Wan
Yuan-lian
YL
Liu
Yu-cun
YC
Yao
Hong-wei
HW
Wang
Xin
X
Wu
Tao
T
Pan
Yi-sheng
YS
Zhu
Jing
J
D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D018076:DNA, Complementary; D013925:Thromboplastin