[Inhibitory effect against the gastric carcinoma cell growth by the combination of the survivin antisense oligonucleotide and P53 gene].
OBJECTIVE
To investigate the inhibitory effect and mechanism against the growth of human gastric carcinoma cell line HS-746T by the combination of the survivin antisense oligonucleotide (ASODN) and P53 gene and its mechanism.


METHODS
Gastric carcinoma cell line HS-746T was treated by P53 gene and survivin antisense oligonucleotide was designed. There were four regimen groups treated by different agents:ASODN alone, P53 gene alone and the combination of ASODN and P53 gene, blank control. Cell proliferative ability and cell growth were determined by cells counting and MTT. The expression of survivin mRNA and protein was detected by RT-PCR and Western blot respectively. Cell apoptotic index was detected by TUNEL.


RESULTS
ASODN alone, P53 alone and the combination of ASODN and P53 could inhibit not only the growth of gastric carcinoma cell, but also down-regulate the survivin mRNA and protein expression. The inhibitory effect was stronger, and the apoptosis index was higher in the combined transfection group than those in the other two single transfection groups.


CONCLUSIONS
The combination of survivin ASODN and P53 gene is more efficient to inhibit cell growth and induce apoptosis than that of agent alone.
Fu
Guang
G
Department of General Surgery, Wugang Hospital, Wuhan 430080, China. fuguang71@hotmail.com
Wang
Guo-bin
GB
Lu
Xiao-ming
XM
Xia
Yu-chun
YC
Yang
Chun
C
Tian
Ya-liang
YL
D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D016376:Oligonucleotides, Antisense / Q000235:genetics* / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D000077022:Survivin; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D016376:Oligonucleotides, Antisense; D000077022:Survivin; D016159:Tumor Suppressor Protein p53