Quantitative analysis of intracellular metabolic fluxes using GC-MS and two-dimensional NMR spectroscopy.
A method for metabolic flux determination by carbon labeling experiments is described. The cells were grown on 13C-labeled substrate, and the labeling patterns of the amino acids in biomass hydrolysate were measured using two dimensional 1H-13C correlation nuclear magnetic resonance spectroscopy (2D 1H-13C COSY NMR) and gas chromatography/mass spectrometry (GC-MS) technique. The estimation of intracellular flux distribution from the measured labeling data and extracellular fluxes was achieved by a nonlinear least squares fitting approach, since a framework was developed that allows mathematical description relating the measured quantities and the intracellular fluxes. The described method was used to investigate the central carbon metabolism of the mixotrophically cultivated Synechocystis sp. PCC6803. The statistical quality of the estimated fluxes was also evaluated.
Yang
Chen
C
Department of Biochemical Engineering & Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan.
Hua
Qiang
Q
Shimizu
Kazuyuki
K