title: Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein.
abstract: We describe a novel homogeneous protein-protein interaction assay based on bioluminescence resonance energy transfer (BRET) from mutant firefly luciferase to red fluorescent protein (DsRed). The association of glutathione S-transferase (GST)-luciferase and protein G-DsRed, which was driven by the addition of anti-GST antibody, was successfully monitored by BRET.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Arai
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kitayama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueda
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nagamune