Process development for industrial-scale preparation of carboxypeptidase Y secreted by Saccharomyces cerevisiae.
A method for industrial-scale preparation of carboxypeptidase Y (CPY), which was secreted by Saccharomyces cerevisiae KS58-2D/pCY303 into the culture broth, was developed. Because the purification process consists of a few simple unit operations including only one chromatography step, a higher CPY recovery was achieved than that in the process using disrupted baker's yeast. Approximately 100 g of purified CPY powder was constantly obtained using the final culture broth from a 500-l fermentor.
Shiba
Y
Y
Lead Discovery Research Laboratories, Sankyo Co. Ltd., 389-4 Aza-Ohtsurugi, Shimokawa, Izumimachi, Iwaki, Fukushima 971-8183, Japan.
Kanari
Y
Y
Fukui
F
F
Yoshikawa
H
H