title: [Changes of heat-shock protein 70 in the brain of rabbits with craniocerebral missile injury in hot and humid environment].
abstract: OBJECTIVE
  To study the changes of heat shock protein 70 (HSP70) in the brain of rabbits with craniocerebral missile injury (CMI) and their impact on the injury in a hot and humid environment (HHE).
  
  
  METHODS
  Twenty-four New Zealand white rabbits were randomized into 3 equal groups, namely normal temperature (NT) control group (subjected to treatment at temperature of 22.0+/-0.5 degrees C with relative humidity of 50%), NT gunshot group with CMI and HHE gunshot group with CMI. The rabbits in the latter two groups were subjected to normal temperature of 22.0+/-0.5 degrees C with relative humidity of 50% and environmental temperature of 39.0+/-0.5 degrees C with relative humidity of 80% to 85%, respectively, after establishment of CMI models. HSP70 in the brain tissues and lymphocytes was detected by Western blotting and visualized using chemoluminescence and X-ray films, followed by quantitative gel image analysis.
  
  
  RESULTS
  Expressions of HSP70 in the cortex, hypothalamus and lymphocytes increased apparently in HHE gunshot group, and the changes of HSP70 in the lymphocytes were consistent with those in the cerebral cortex and hypothalamus.
  
  
  CONCLUSIONS
  HSP70 expression in the brain tissues and the lymphocytes increase evidently after MCI in HHE.
authors.affiliation: Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. huangqijin@hotmail.com
authors.forename: Qi-jin
authors.initials: QJ
authors.lastname: Huang
authors.forename: Ru-xiang
authors.initials: RX
authors.lastname: Xu
authors.forename: Yi-quan
authors.initials: YQ
authors.lastname: Ke
mesh_terms: D000818:Animals; D002980:Climate; D006259:Craniocerebral Trauma / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006358:Hot Temperature; D006813:Humidity; D011817:Rabbits; D014948:Wounds, Gunshot / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins