title: Heteromerization and colocalization of TrpV1 and TrpV2 in mammalian cell lines and rat dorsal root ganglia.
abstract: Coassociation of the vanilloid transient receptor potential (Trp) ion channels, TrpV1 and TrpV2, was investigated by immunoprecipitation and immunofluorescence in transfected mammalian cell lines, rat dorsal root ganglia and spinal cord. TrpV1/TrpV2 heteromeric complexes were coimmunoprecipitated from human embryonic kidney cells and F-11 dorsal root ganglion hybridoma cells following their transient coexpression. Immunofluorescent labelling of transfected F-11 cells revealed colocalization of TrpV1 and TrpV2 at the cell surface. Immunoprecipitation from rat dorsal root ganglion lysates identified a minor population of receptor complexes composed of TrpV1/TrpV2 heteromers, consistent with a small proportion of cells double-labelled with TrpV1 and TrpV2 antibodies in rat dorsal root ganglion sections. TrpV1/TrpV2 receptor complexes may represent a functionally distinct ion channel complex that may increase the diversity observed within the Trp ion channel family.
authors.affiliation: Department of Molecular and Cellular Neuroscience, Neuroscience Research Centre, Merck Sharp and Dohme Research Laboratories, Harlow, Essex, UK. richard_rutter@merck.com
authors.forename: A Richard
authors.initials: AR
authors.lastname: Rutter
authors.forename: Qing-Ping
authors.initials: QP
authors.lastname: Ma
authors.forename: Mathew
authors.initials: M
authors.lastname: Leveridge
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Bonnert
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002460:Cell Line / Q000378:metabolism; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique / Q000379:methods; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D008297:Male; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D050916:TRPV Cation Channels / Q000378:metabolism*; D014162:Transfection / Q000379:methods
chemical_list: D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; C476722:Trpv2 protein, rat