title: Differential use of the two high-oxygen-affinity terminal oxidases of Brucella suis for in vitro and intramacrophagic multiplication.
abstract: Expression of the high-oxygen-affinity cytochrome cbb3 and cytochrome bd ubiquinol oxidases of Brucella suis was studied in vitro and in the intramacrophagic niche, which was previously proposed to be oxygen limited. The cytochrome cbb3 oxidase was exclusively expressed in vitro, whereas the cytochrome bd oxidase was preferentially used inside macrophages and contributed to intracellular bacterial replication.
authors.affiliation: INSERM U-431, Université Montpellier II, Place E. Bataillon, CC100, 34095 Montpellier Cedex 05, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Loisel-Meyer
authors.forename: Maria Pilar
authors.initials: MP
authors.lastname: Jiménez de Bagüés
authors.forename: Stephan
authors.initials: S
authors.lastname: Köhler
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Liautard
authors.forename: Véronique
authors.initials: V
authors.lastname: Jubier-Maurin
mesh_terms: D000332:Aerobiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D041021:Brucella suis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D002460:Cell Line; D049109:Cell Proliferation; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D010088:Oxidoreductases; C414443:cbb3 oxidase; D003576:Electron Transport Complex IV; D010100:Oxygen