Immobilization of active hydrogenases by encapsulation in polymeric porous gels.
Hydrogenases encapsulated in porous polymeric silica gels retain significant levels of hydrogen production activity when compared to hydrogenases in solution using reduced methyl viologen as an electron donor. Encapsulated hydrogenases remain active after storage at room temperature for longer than four weeks and are less sensitive to proteolytic digestion. Nanoscopic confinement of active hydrogenases in solids paves the way for their potential use in hydrogen producing catalytic materials applications.
Elgren
Timothy E
TE
Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA. TElgren@Hamilton.edu
Zadvorny
Oleg A
OA
Brecht
Eric
E
Douglas
Trevor
T
Zorin
Nikolay A
NA
Maroney
Michael J
MJ
Peters
John W
JW
D002214:Capsules / Q000737:chemistry; D003013:Clostridium / Q000201:enzymology; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D006864:Hydrogenase / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013696:Temperature; D020616:Thiocapsa roseopersicina / Q000201:enzymology
D002214:Capsules; D004800:Enzymes, Immobilized; D005782:Gels; D011108:Polymers; D006864:Hydrogenase