title: Expression of a major house dust mite allergen gene from Dermatophagoides farinae in Lotus japonicus accession Miyakojima MG-20.
abstract: Transformation of a model legume Lotus japonicus accession Miyakojima MG-20 was examined using Agrobacterium tumefaciens with a binary expression vector. Using the improved transformation method, we introduced a major allergen gene from a house dust mite, Der f 1, into MG-20. Analyses by Southern hybridization, reverse transcription (RT)-PCR, and Western blotting showed that the Der f 1 gene was integrated into the genome of L. japonicus, expressing the gene product in the T1 lines. Our results imply future application of oral allergen-specific immunotherapy using legume plants.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Kato
authors.forename: Yuji
authors.initials: Y
authors.lastname: Goto
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ono
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Murooka
mesh_terms: D039741:Antigens, Dermatophagoides / Q000096:biosynthesis* / Q000235:genetics*; D060829:Arthropod Proteins; D003001:Cloning, Molecular / Q000379:methods*; D003546:Cysteine Endopeptidases; D040001:Dermatophagoides farinae / Q000235:genetics / Q000378:metabolism; D000070116:Lotus / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D011994:Recombinant Proteins; D003546:Cysteine Endopeptidases; C451159:Dermatophagoides farinae antigen f 1