title: Reversibility of hepatocyte nuclear modifications in mice fed on genetically modified soybean.
abstract: In the literature, the reports on the effects of a genetically modified (GM) diet are scanty and heterogeneous; in particular, no direct evidence has so far been reported that GM food may affect human or animal health. Hepatocytes represent a suitable model for monitoring the effects of a GM diet, the liver potentially being a primary target. In a previous study, we demonstrated that some modifications occur in hepatocyte nuclei of mice fed on GM soybean. In order to elucidate whether such modifications can be reversed, in the present study, 3 months old mice fed on GM soybean since their weaning were submitted to a diet containing wild type soybean, for one month. In parallel, to investigate the influence of GM soybean on adult individuals, mice fed on wild type soybean were changed to a GM diet, for the same time. Using immunoelectron microscopy, we demonstrated that a one-month diet reversion can influence some nuclear features in adult mice, restoring typical characteristics of controls in GM-fed animals, and inducing in control mice modifications similar to those observed in animals fed on GM soybean from weaning. This suggests that the modifications related to GM soybean are potentially reversible, but also that some modifications are inducible in adult organisms in a short time.
authors.affiliation: Istituto di Istologia e Analisi di Laboratorio, University of Urbino Carlo Bo, via Zeppi s.n., 61029 Urbino, Italy. malatesta@uniurb.it
authors.forename: M
authors.initials: M
authors.lastname: Malatesta
authors.forename: C
authors.initials: C
authors.lastname: Tiberi
authors.forename: B
authors.initials: B
authors.lastname: Baldelli
authors.forename: S
authors.initials: S
authors.lastname: Battistelli
authors.forename: E
authors.initials: E
authors.lastname: Manuali
authors.forename: M
authors.initials: M
authors.lastname: Biggiogera
mesh_terms: D000375:Aging; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004032:Diet; D005260:Female; D030841:Food, Genetically Modified / Q000009:adverse effects*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D011247:Pregnancy; D013025:Glycine max / Q000235:genetics