Decolorization of molasses wastewater by Bacillus sp. under thermophilic and anaerobic conditions.
Various microorganisms were screened for their ability to decolorize molasses wastewater under thermophilic and anaerobic conditions. Strain MD-32, which was newly isolated from a soil sample, was selected as the best strain. From taxonomical studies, the strain was concluded to belong to the genus Bacillus, most closely resembling B. smithii. The strain decolorized 35.5% of molasses pigment within 20 d at 55 degrees C under anaerobic conditions, but no decolorization activity was observed when it was cultivated aerobically. At all the concentrations tested molasses pigment was effectively decolorized by MD-32, with decolorization yields of around 15% within 2 d. The molecular weight distribution as determined by gel filtration chromatography revealed that the decolorization of molasses pigment by the isolated strain is accompanied by a decrease in not only small molecules but also large ones.
Nakajima-Kambe
T
T
Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Shimomura
M
M
Nomura
N
N
Chanpornpong
T
T
Nakahara
T
T