Incorporation of spin-labelled amino acids into proteins.
The elucidation of structure and function of proteins and membrane proteins by EPR spectroscopy has become increasingly important in recent years as technological advances have been made in the design of spectrometers and in the chemistry of the nitroxide group. These new developments have increased the demand for tailor-made amino acids carrying a spin label on the one hand and for reliable methods for their incorporation into proteins on the other. Here we describe methods for site-specific spin labelling of proteins. It is shown that a combination of recombinant synthesis of proteins with chemically produced peptides (expressed protein ligation) allows the preparation of site-specifically spin-labelled proteins.
Becker
Christian F W
CF
Max-Planck-Institut für Molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany.
Lausecker
Kester
K
Balog
Mária
M
Kálai
Tamás
T
Hideg
Kálmán
K
Steinhoff
Heinz-Jürgen
HJ
Engelhard
Martin
M
D000596:Amino Acids / Q000138:chemical synthesis / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D015394:Molecular Structure; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D013113:Spin Labels
D000596:Amino Acids; D011506:Proteins; D013113:Spin Labels