title: The immunohistochemical approach to determine the origin and possible function of the juxtaoral organ in dogs.
abstract: OBJECTIVE
  In this study, we applied immuno- histochemical techniques on the functionally little known organ of Chievitz (juxtaoral organ [JOO]) in dogs to determine its origin and possible function.
  
  
  METHODS
  The term abortive materials of 6 Doberman dogs were used for experimental procedures in July 2002 to June 2003 at Gazi University Faculty of Medicine, Ankara, Turkey, after routine light microscopic tissue preparation, the sections were stained with Masson's trichrome stain. In order to elucidate the function-related origin of the organ, we used epidermal growth factor (EGF-r), transforming growth factor (TGF-alpha) and nerve growth factor (NGF-beta) immunohistochemical stains.
  
  
  RESULTS
  We observed a very strong and widespread immunoreactivity of EGF-r and TGF-alpha on simple squamous capsular cells. We detected nerve growth factor-beta positivity in granular form both in simple squamous capsular cells and in neighboring connective tissue. However, we did not detect EGF-r reactivity on parenchymal cells except a weak immunoreactivity on central ones. We noticed transforming growth factor-alpha in most of the parenchymal cells while we observed NGF-beta strongly in all the parenchymal cells.
  
  
  CONCLUSIONS
  These results may point out that the JOO may be of mesothelial or epithelial origin. Having NGF-alpha positive granules and close relationship with blood vessels may imply a neurosecretory function. We believe that our study may add new perspectives to the function of the JOO.
authors.affiliation: Department of Anatomy, School of Medicine, Faculty of Medicine, Gazi University, Ankara, Turkey. meltemb@gazi.edu.tr
authors.forename: Meltem
authors.initials: M
authors.lastname: Bahcelioglu
authors.forename: Engin
authors.initials: E
authors.lastname: Calguner
authors.forename: Deniz
authors.initials: D
authors.lastname: Erdogan
authors.forename: Cigdem
authors.initials: C
authors.lastname: Elmas
authors.forename: Rabet
authors.initials: R
authors.lastname: Gozil
authors.forename: Semih
authors.initials: S
authors.lastname: Keskil
authors.forename: Dural
authors.initials: D
authors.lastname: Kadioglu
mesh_terms: D000818:Animals; D001707:Biopsy, Needle; D004285:Dogs; D004815:Epidermal Growth Factor; D005260:Female; D007150:Immunohistochemistry; D023421:Models, Animal; D011247:Pregnancy; D011270:Pregnancy, Animal; D012680:Sensitivity and Specificity; D013704:Temporomandibular Joint / Q000196:embryology* / Q000473:pathology*; D016211:Transforming Growth Factor alpha
chemical_list: D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor