The influence of methylene blue on the spontaneous contractity of the non-pregnant human myometrium and on the myometrial response to DEA/NO.
Nitric oxide (NO) is a potent inhibitor of spontaneous contractions of the human non-pregnant myometrium; however, the precise mechanism by which NO causes the myometrial smooth muscles to relax remains unclear. The aim of this study was to determine the influence of methylene blue (MB) on myometrial contractions and the response of the myometrium to DEA/NO in vitro. Concentration-response curves to DEA/NO were constructed in the absence and presence of MB (5x10(-6), 10(-4) and 10(-2) mol/l) and 5x10(-3) mol/l cystamine. Cystamine did not counteract the DEA/NO-induced relaxation of the myometrial strips. MB itself, excluding the lowest concentration, caused noticeable changes in spontaneous activity. The changes involved a concentration-dependent increase in the frequency of contractions, and a decrease in their amplitude. In conclusion, our results confirm that NO relaxes the human myometrium via a cGMP-independent mechanism. The results obtained in the presence of MB may be misleading because of its complex influence on myometrial contractile activity.
Modzelewska
Beata
B
Department of Biophysics, Medical University of Białystok, ul. Mickiewicza 2A, 15-230 Białystok, Poland. beamo@amb.edu.pl
Kostrzewska
Anna
A
D000328:Adult; D019540:Area Under Curve; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D006834:Hydrazines / Q000494:pharmacology*; D007537:Isometric Contraction / Q000187:drug effects*; D008751:Methylene Blue / Q000494:pharmacology*; D008875:Middle Aged; D009215:Myometrium / Q000187:drug effects*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009589:Nitrogen Oxides / Q000494:pharmacology*; D017697:Premenopause; D014590:Uterine Contraction / Q000187:drug effects*
D006834:Hydrazines; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; C084012:1,1-diethyl-2-hydroxy-2-nitrosohydrazine; D008751:Methylene Blue