title: [Extracellular proteolytic enzymes of Azospirillum brasilensis strain Sp7 and regulation of their activity by a homologous lectin].
abstract: It was found that Azospirillum brasilensis strain Sp7 is able to produce extracellular proteolytic enzymes. The enzymes were active within a broad range of pH values, with two peaks of activity being located in the acid and alkaline pH areas; required calcium ions; and exhibited substrate specificity with respect to azogelatin. Zymography allowed at least four proteolytic enzymes with molecular weights of 32, 45, 52, and 174 kDa to be detected in A. brasilense Sp7 culture liquid. It was shown that the lectin from A. brasilense Sp7 can inhibit proteolytic enzymes.
authors.forename: M P
authors.initials: MP
authors.lastname: Chernyshova
authors.forename: S A
authors.initials: SA
authors.lastname: Alen'kina
authors.forename: V E
authors.initials: VE
authors.lastname: Nikitina
authors.forename: V V
authors.initials: VV
authors.lastname: Ignatov
mesh_terms: D016949:Azospirillum / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D037102:Lectins / Q000502:physiology*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D013379:Substrate Specificity
chemical_list: D037102:Lectins; D011480:Protease Inhibitors; D010447:Peptide Hydrolases