Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1.
We developed a model system whereby HP1 can be targeted to pericentric heterochromatin in ES cells lacking Suv(3)9h1/2 histone methyltransferase (HMTase) activities. HP1 so targeted can reconstitute tri-methylated lysine 9 of histone H3 (Me(3)K9H3) and tri-methylated lysine 20 of histone H4 (Me(3)K20H4) at pericentric heterochromatin, indicating that HP1 can regulate the distribution of these histone modifications in vivo. Both homo- and hetero-typic interactions between the HP1 isotypes were demonstrated in vivo as were HP1 interactions with the ESET/SETDB1 HMTase and the ATRX chromatin remodelling enzyme. We conclude that HP1 not only "deciphers" the histone code but can also "encode it".
Kourmouli
Niki
N
Division of Tumor Biology, Department of Immunology and Cell Biology, Forschungszentrum Borstel, D-23845 Borstel, Germany.
Sun
Yuh-man
YM
van der Sar
Sjaak
S
Singh
Prim B
PB
Brown
Jeremy P
JP
D000818:Animals; D002460:Cell Line; D002503:Centromere / Q000235:genetics* / Q000378:metabolism; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D044127:Epigenesis, Genetic / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006570:Heterochromatin / Q000235:genetics* / Q000378:metabolism; D042421:Histone Code / Q000235:genetics; D006657:Histones / Q000235:genetics*; D051379:Mice; D013234:Stem Cells / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; D006570:Heterochromatin; D006657:Histones; D000090266:Chromobox Protein Homolog 5