title: Proteomic dataset of Sca-1+ progenitor cells.
abstract: Embryonic stem cells (ES cells) can differentiate into endothelial cells and smooth muscle cells (SMCs), which participate in vascular angiogenesis. In this study, we differentiated mouse ES cells into Sca-1(+) cells, which have the potential to serve as vascular progenitor cells, and mapped their proteome by 2-DE using a pH 3-10 non-linear gradient and 12% SDS-polyacrylamide gels. A subset of 300 protein spots was analysed and mapped, with 241 protein spots being identified by their PMF using MALDI-TOF MS or by partial amino acid sequencing using MS/MS. Our protein map is the first of Sca-1(+) progenitor cells and will facilitate the identification of proteins differentially expressed during stem cell differentiation. The proteome of adult arterial SMCs is described in an accompanying paper (in this issue, DOI 10.1002/pmic.200402045). All data are made accessible on our website http://www.vascular-proteomics.com.
authors.affiliation: Department of Cardiac and Vascular Sciences, St. George's, University of London, UK.
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Yin
authors.forename: Manuel
authors.initials: M
authors.lastname: Mayr
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Xiao
authors.forename: Ursula
authors.initials: U
authors.lastname: Mayr
authors.forename: Edward
authors.initials: E
authors.lastname: Tarelli
authors.forename: Robin
authors.initials: R
authors.lastname: Wait
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Xu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004396:Coloring Agents; D017931:DNA Primers; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry; D051379:Mice; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013234:Stem Cells / Q000737:chemistry* / Q000166:cytology
chemical_list: D004396:Coloring Agents; D017931:DNA Primers; D004798:Enzymes; D011506:Proteins; D020543:Proteome