title: Distinction of Leu and Ile Using a Ruthenium(II) complex by MALDI-LIFT-TOF/TOF-MS analysis.
abstract: The novel N-terminal labeling method using a ruthenium(II) complex derivative characteristically indicated a(n) and d(n) (N-terminal) fragment ions in high sensitivity by MS/MS analysis (MALDI-LIFT or ESI-CID). Although these fragment ions depended on a fragmentation process by MS/MS analytical methods to some degree, each case indicated similar side-chain cleavage patterns. The labeling method allows accurate distinction of amino acid residues by MS/MS analysis even if the residues are structural isomers such as leucine and isoleucine. The method was applied to long-chain peptides and provided easy and rapid N-terminal sequencing.
authors.affiliation: Department of Macromolecular Science and Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ito
authors.forename: Taka-Aki
authors.initials: TA
authors.lastname: Okamura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Norikazu
authors.initials: N
authors.lastname: Ueyama
authors.forename: Kojiro
authors.initials: K
authors.lastname: Ake
authors.forename: Ryoji
authors.initials: R
authors.lastname: Masui
authors.forename: Seiki
authors.initials: S
authors.lastname: Kuramitsu
authors.forename: Susumu
authors.initials: S
authors.lastname: Tsunasawa
mesh_terms: D007532:Isoleucine / Q000737:chemistry*; D007930:Leucine / Q000737:chemistry*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D009942:Organometallic Compounds; D007532:Isoleucine; D012428:Ruthenium; D007930:Leucine