title: Estimation of the biofilm formation of Escherichia coli K-12 by the cell number.
abstract: We developed a method of estimating the biofilm formation of Escherichia coli K-12 strains in microtiter-plate wells by the cell number. Regression lines between the cell number and absorbance of crystal violet that stained the E. coli biofilm consisted of high and low slope lines, respectively.
authors.affiliation: Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa-shi 252-8510, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Narisawa
authors.forename: Soichi
authors.initials: S
authors.lastname: Furukawa
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ogihara
authors.forename: Makari
authors.initials: M
authors.lastname: Yamasaki
mesh_terms: D000465:Algorithms; D018441:Biofilms / Q000254:growth & development*; D015169:Colony Count, Microbial / Q000379:methods*; D048168:Escherichia coli K12 / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology*; D005840:Gentian Violet; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D005840:Gentian Violet