Estimation of the biofilm formation of Escherichia coli K-12 by the cell number.
We developed a method of estimating the biofilm formation of Escherichia coli K-12 strains in microtiter-plate wells by the cell number. Regression lines between the cell number and absorbance of crystal violet that stained the E. coli biofilm consisted of high and low slope lines, respectively.
Narisawa
Naoki
N
Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa-shi 252-8510, Japan.
Furukawa
Soichi
S
Ogihara
Hirokazu
H
Yamasaki
Makari
M
D000465:Algorithms; D018441:Biofilms / Q000254:growth & development*; D015169:Colony Count, Microbial / Q000379:methods*; D048168:Escherichia coli K12 / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology*; D005840:Gentian Violet; D013057:Spectrum Analysis / Q000379:methods*
D005840:Gentian Violet