Application of an optimization algorithm for estimating intrinsic kinetic parameters of immobilized enzymes.
A simple optimization methodology is applied to estimate the intrinsic kinetic parameters for both reversible and irreversible unireactant immobilized enzyme systems that follow the Michaelis-Menten mechanism. The method utilizes a direct-search optimization algorithm along with the numerical solution of the governing differential equations. The usefulness and validity of the method is demonstrated by comparing the predicted values of the intrinsic kinetic constants using the proposed method with a series of experimental values reported in the literature for different immobilized enzyme systems with irreversible and reversible reactions.
Khorasheh
Farhad
F
Department of Chemical Engineering, Sharif University of Technology, Tehran, Iran. khorashe@sharif.ac.ir
Kheirolomoom
Azadeh
A
Mireshghi
Seyedalireza
S