Some advantages of high temperature for the separation of pharmaceutical compounds with mass spectrometry detection.
The beneficial effects of high temperature on separation and detection of basic compounds, the detection being performed by MS via ESI, are investigated. The influence of various parameters on both separation and detection performances is studied. These parameters include the mobile phase pH, the temperature, and the type of stationary phase. Experiments are performed under gradient elution conditions. The results obtained with four different supports, silica-, zirconia-, carbon-, and polymer-based columns, are compared by means of different criteria including the elution composition, the peak asymmetry, and the S/N. High temperature liquid chromatography at high pH with volatile buffers suitable for MS detection was shown to be an interesting choice for solutes with basic sites.
Albert
Monique
M
Laboratoire des Sciences Analytiques, Université Claude Bernard-Lyon 1, Villeurbanne, France.
Cretier
Gérard
G
Guillarme
Davy
D
Heinisch
Sabine
S
Rocca
Jean-Louis
JL
D002853:Chromatography, Liquid / Q000379:methods; D003061:Codeine / Q000737:chemistry / Q000302:isolation & purification; D004155:Diphenhydramine / Q000737:chemistry / Q000302:isolation & purification; D007202:Indicators and Reagents; D013058:Mass Spectrometry / Q000379:methods; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000302:isolation & purification*; D011343:Procaine / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D011433:Propranolol / Q000737:chemistry / Q000302:isolation & purification; D011803:Quinine / Q000737:chemistry / Q000302:isolation & purification; D013816:Thermodynamics
D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D011343:Procaine; C004616:chloroprocaine; D004155:Diphenhydramine; D011433:Propranolol; D011803:Quinine; D003061:Codeine