[Identification of up-regulated genes induced by angiotensin II in cardiac fibroblasts].
To identify up-regulated genes in adult rat cardiac fibroblasts (CF) induced by angiotensin II (Ang II), suppression subtractive hybridization (SSH) was performed between the CF stimulated by Ang II (tester) and unstimulated CF (driver) to generate subtractive cDNA library. The library was screened with dot blots hybridization to further verify the differentially expressed cDNA clones. Partial positive clones (19 up-regulated genes) were sequenced and BLAST analyzed. Twelve up-regulated genes related to extracellular matrix, cell cycle, intracellular signal transduction, cell cytoskeleton, cell metabolism and 7 new expressed sequence tags (EST) were acquired (GenBank accession number: CN382808, CN382809, CN382810, CN382811, CN382812, CN382813, CN382814). Our data reveal that SSH is a powerful technique of high sensitivity for the detection and cloning of up-regulated genes expressed in CF induced by Ang II, which may be helpful to clarify the mechanism of cardiac remodeling.
Wang
Xin-Feng
XF
Department of Pathophysiology, Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
Gao
Guang-Dao
GD
Yang
Yu-Bai
YB
Zhou
Juan
J
Wang
Ya-Wen
YW
Su
Xing-Li
XL
Wang
Yan
Y
Han
Feng-Chan
FC
Bai
Yu-Jie
YJ
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D005347:Fibroblasts / Q000166:cytology*; D005786:Gene Expression Regulation; D008297:Male; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D020257:Ventricular Remodeling / Q000235:genetics
D018076:DNA, Complementary; D000804:Angiotensin II