title: Novel sites of cytosolic glutathione peroxidase expression in colon.
abstract: Glutathione peroxidases (Gpx) are important moderators of oxidative stress that is implicated in the pathogenesis of numerous diseases including colon cancer. Previous studies report limited examinations of cytosolic glutathione peroxidase location of expression in colon tissue. This study reports evidence of both common sites of Gpx1 and Gpx2 expression in rat colon and sites that are exclusive to each isoform. Semi-quantitative PCR performed previously demonstrated RNA expression of Gpx1 and Gpx2 in proximal, transverse and distal colon. Mapping the distribution throughout the entire colon has revealed specific, novel sites of glutathione peroxidase expression in colon lymphatic tissue. In situ hybridisation and immunohistochemistry confirmed micro-anatomical location of Gpx1 within lymphatic tissue and the lamina propria, sub-mucosa, muscularis and serosa, but not the lumenal epithelium. In situ hybridisation and immunohistochemistry were consistent with reports of microanatomical location of Gpx2 in the lumenal epithelium. Novel sites of Gpx2 expression were also observed in lymphatic tissue. Immunolocalisation in the vicinity of aberrant crypt foci was also examined to further investigate the link between glutathione peroxidases and colon cancer. This did not reveal significant abnormalities, nor did measurement of cytosolic glutathione peroxidase activity or gene expression in colon tissue from rats treated with the colontropic chemical, 1,2-dimethylhydrazine. These results support the potential for Gpx1 and Gpx2 redundancy in lymphatic tissue, but not in epithelial cells of the colon crypt or in the lamina propria, sub-mucosa, muscularis or serosa.
authors.affiliation: Gut Health Division, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK. jed@rri.sari.ac.uk
authors.forename: Janice E
authors.initials: JE
authors.lastname: Drew
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Farquharson
authors.forename: John R
authors.initials: JR
authors.lastname: Arthur
authors.forename: Philip C
authors.initials: PC
authors.lastname: Morrice
authors.forename: Garry G
authors.initials: GG
authors.lastname: Duthie
mesh_terms: D000818:Animals; D003106:Colon / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology*; D015870:Gene Expression; D005979:Glutathione Peroxidase / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D008221:Lymphoid Tissue / Q000201:enzymology; D010544:Peroxidases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D014018:Tissue Distribution; D000094765:Glutathione Peroxidase GPX1
chemical_list: D012333:RNA, Messenger; D010544:Peroxidases; C027585:glutathione peroxidase II; D005979:Glutathione Peroxidase; D000094765:Glutathione Peroxidase GPX1; C000722069:Gpx1 protein, rat