title: Evolution of the receptor binding phenotype of influenza A (H5) viruses.
abstract: Receptor specificity of influenza A/H5 viruses including human 2003-04 isolates was studied. All but two isolates preserved high affinity to Sia2-3Gal (avian-like) receptors. However, two isolates (February, 2003, Hong Kong) demonstrated decreased affinity to Sia2-3Gal and moderate affinity to a Sia2-6Gal (human-like) receptors. These two viruses had a unique Ser227-Asn change in the hemagglutinin molecule. Thus, a single amino acid substitution can significantly alter receptor specificity of avian H5N1 viruses, providing them with an ability to bind to receptors optimal for human influenza viruses. Asian 2003-04 H5 isolates from chickens and humans demonstrated highest affinity to the sulfated trisaccharide Neu5Acalpha2-3Galbeta1-4(6-HSO3)GlcNAcbeta (Su-3'SLN) receptor but, in contrast to 1997 isolates, had increased affinity to fucosylated Su-3'SLN. American poultry H5 viruses also had increased affinity to Su-3'SLN. These data demonstrate that the genetic evolution of avian influenza A(H5N1) viruses is accompanied during adaptation to poultry by the evolution of their receptor specificity.
authors.affiliation: Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, 142782 Moscow, Russia. gambar@com2com.ru
authors.forename: Alexandra
authors.initials: A
authors.lastname: Gambaryan
authors.forename: Alexander
authors.initials: A
authors.lastname: Tuzikov
authors.forename: Galina
authors.initials: G
authors.lastname: Pazynina
authors.forename: Nicolai
authors.initials: N
authors.lastname: Bovin
authors.forename: Amanda
authors.initials: A
authors.lastname: Balish
authors.forename: Alexander
authors.initials: A
authors.lastname: Klimov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D019143:Evolution, Molecular; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism; D053124:Influenza A Virus, H5N1 Subtype / Q000378:metabolism*; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D011485:Protein Binding; D011487:Protein Conformation; D011991:Receptors, Virus / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D006020:Glycopeptides; D011991:Receptors, Virus