Accumulation of S-2-hydroxyethyl derivatives of L-cysteine and L-homocysteine by a methionine auxotroph of Escherichia coli with the addition of 2-mercaptoethanol to the culture.
We previously reported [J. Biosci. Bioeng., 94, 178-181 (2002)] that an Escherichia coli MetC-deficient mutant can accumulate L-cystathionine. When 2-mercaptoethanol was added to the culture medium during fermentation, the accumulation of L-cystathionine decreased and S-(2-hydroxyethyl)-L-cysteine and S-(2-hydroxyethyl)-L-homocysteine were accumulated.
Nishi
Takuya
T
Graduate School of Integrated Science and Art, University of East Asia, 2-1 Ichinomiya-gakuenmachi, Shimonoseki City, Yamaguchi 751-0807, Japan.
Kouda
Noriko
N
Watanabe
Kikuko
K
Tanaka
Yoshitake
Y
Araki
Kazumi
K
Furihata
Kazuo
K
Onodera
Masayuki
M
Toda
Kiyoshi
K