title: Enantiomeric separation of 1-phenylethylamine and 1-cyclohexylethylamine in capillary electrophoresis with contactless conductivity detection.
abstract: Contactless conductivity detection was employed for the detection of the enantiomers of 1-phenylethylamine and 1-cyclohexylethylamine which were separated in capillary electrophoresis with unprecedented high resolutions R(s) of 2.3 and 3.3, respectively, by using a combination of dimethyl-beta-cyclodextrin and the chiral crown ether 18C6H4 as chiral selectors in a citric acid buffer of pH 2.4. The conductivity measurement enabled the direct detection, i.e. without having to derivatize or resort to indirect methods, of all species including the non-UV-absorbing enantiomers of cyclohexylamine. Detection limits of 0.5 microM were achieved and the determination of enantiomeric ratios of up to 99:1 was found possible.
authors.affiliation: Department of Chemistry, University of Basel, Spitalstrasse 51, 4004 Basel, Switzerland.
authors.forename: Xiao Yang
authors.initials: XY
authors.lastname: Gong
authors.forename: Peter C
authors.initials: PC
authors.lastname: Hauser
mesh_terms: D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D005021:Ethylamines / Q000737:chemistry / Q000302:isolation & purification*; D010627:Phenethylamines / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
chemical_list: D005021:Ethylamines; D010627:Phenethylamines; C080149:1-cyclohexylethylamine