Stress-dependent and -independent expression of the myogenic regulatory factors and the MARP genes after eccentric contractions in rats.
The relationship between muscle mechanical conditions and gene expression was investigated by varying both stress and contraction mode imposed upon rat dorsiflexors (n= 25), activating them at high or low frequencies (150 Hz or 40 Hz) either eccentrically or isometrically. Muscle physiological, immunohistochemical and gene expression changes were then measured 24 h after the exercise bout. Peak stress was the best predictor of muscle injury, independent of contraction mode (i.e. eccentric or isometric). When peak stresses were matched, no physiological or immunohistochemical differences were detected between isometric and eccentric contractions. The expression of certain myogenic regulatory and muscle ankyrin repeat protein (MARP) genes (myoD, myogenin, MLP and CARP) depended both on peak muscle stress achieved during contraction and contraction mode. In contrast, Arpp/Ankrd2 was dramatically upregulated only by eccentric contractions, but not by isometric contractions, even though the stress level of the eccentric contractions varied over a three-fold range and overlapped with that of the isometric group. The role that Arpp/Ankrd2 upregulation plays in the biological response to eccentric contraction remains to be determined, as does the control mechanism whereby the expression of certain genes (such as myoD, myogenin, MLP and CARP) is sensitive to muscle stress while another (Arpp/Ankrd2) is sensitive only to contraction mode.
Hentzen
Eric R
ER
Department of Orthopaedic Surgery, University of California and Veterans Administration Medical Centers, San Diego, CA 92161, USA.
Lahey
Michele
M
Peters
David
D
Mathew
Liby
L
Barash
Ilona A
IA
Fridén
Jan
J
Lieber
Richard L
RL
D000818:Animals; D003893:Desmin / Q000378:metabolism; D005353:Fibronectins; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D007537:Isometric Contraction; D060588:LIM Domain Proteins; D008297:Male; D023421:Models, Animal; D015999:Multivariate Analysis; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000293:injuries / Q000378:metabolism; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism; D018008:Myogenin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D013314:Stress, Mechanical; D013997:Time Factors; D014746:Vimentin / Q000378:metabolism
D003893:Desmin; D005353:Fibronectins; D060588:LIM Domain Proteins; D009124:Muscle Proteins; D017570:MyoD Protein; C503803:Myog protein, rat; D018008:Myogenin; D012333:RNA, Messenger; D012097:Repressor Proteins; D014746:Vimentin; C090114:cysteine and glycine-rich protein 3