title: Crystal structure of Methanococcus voltae RadA in complex with ADP: hydrolysis-induced conformational change.
abstract: Members of a superfamily of RecA-like recombinases facilitate a central strand exchange reaction in the DNA repair process. Archaeal RadA and Rad51 and eukaryal Rad51 and meiosis-specific DMC1 form a closely related group of recombinases distinct from bacterial RecA. Nevertheless, all such recombinases share a conserved core domain which carries the ATPase site and putative DNA-binding sites. Here we present the crystal structure of an archaeal RadA from Methanococcus voltae (MvRadA) in complex with ADP and Mg2+ at 2.1 A resolution. The crystallized RadA-ADP filament has an extended helical pitch similar to those of previously determined structures in the presence of nonhydrolyzable ATP analogue AMP-PNP. Structural comparison reveals two recurrent conformations with an extensive allosteric effect spanning the ATPase site and the putative DNA-binding L2 region. Varied conformations of the L2 region also imply a dynamic nature of recombinase-bound DNA.
authors.affiliation: Department of Biochemistry, University of Saskatchewan, A3 Health Sciences Building, 107 Wiggins Road, Saskatoon, Saskatchewan, Canada S7N 5E5.
authors.forename: Xinguo
authors.initials: X
authors.lastname: Qian
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Yujiong
authors.initials: Y
authors.lastname: He
authors.forename: Yu
authors.initials: Y
authors.lastname: Luo
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry*; D000255:Adenosine Triphosphate / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006868:Hydrolysis; D017017:Methanococcus / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C100901:RadA protein, bacteria; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate