The structural and functional role of Med5 in the yeast Mediator tail module.
Med5 (Nut1) is identified here as a component of the Mediator tail region. Med5 is positioned peripherally to Med16 (Sin4) together with the three members of the putative Gal11 module, Med15 (Gal11), Med2, and Med3 (Pgd1). The biochemical analysis receives support from genetic interactions between med5delta and med15delta deletions. The med5delta and med16delta deletion strains share many phenotypes, including effects on mitochondrial function with enhanced growth on nonfermentable carbon sources, increased citrate synthase activity, and increased oxygen consumption. Deletion of the MED5 gene leads to increased transcription of nuclear genes encoding components of the oxidative phosphorylation machinery, whereas mitochondrial genes encoding components of the same machinery are down-regulated. We discuss a possible role for Med5 in coordinating nuclear and mitochondrial gene transcription.
Béve
Jenny
J
Division of Metabolic Diseases, Karolinska Institutet, Novum, Karolinska University Hospital, SE-141 86 Stockholm, Sweden.
Hu
Guo-Zhen
GZ
Myers
Lawrence C
LC
Balciunas
Darius
D
Werngren
Olivera
O
Hultenby
Kjell
K
Wibom
Rolf
R
Ronne
Hans
H
Gustafsson
Claes M
CM
D000818:Animals; D002244:Carbon / Q000737:chemistry; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002850:Chromatography, Gel; D002950:Citrate (si)-Synthase / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry; D015536:Down-Regulation; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D015151:Immunoblotting; D007313:Insecta; D056892:Mediator Complex; D008928:Mitochondria / Q000378:metabolism; D008957:Models, Genetic; D009154:Mutation; D009687:Nuclear Proteins / Q000378:metabolism / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D010101:Oxygen Consumption; D010455:Peptides / Q000737:chemistry; D010641:Phenotype; D010766:Phosphorylation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism / Q000502:physiology*; D013696:Temperature; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
C058337:GAL11 protein, S cerevisiae; C470560:MED2 protein, S cerevisiae; D056892:Mediator Complex; C113769:NUT1 protein, S cerevisiae; D009687:Nuclear Proteins; C110281:PGD1 protein, S cerevisiae; D010455:Peptides; D011994:Recombinant Proteins; C076878:SIN4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D002244:Carbon; D002950:Citrate (si)-Synthase; D012321:DNA-Directed RNA Polymerases; D010100:Oxygen