title: [Expression of hepatitis B surface antigen gene in ginseng cell].
abstract: The recombinant plasmid pBIBSa containing the HBsAg DNA fragment was transferred into Agrobacterium tumefaciens strain LBA4404 directly. Ginseng cells were co-cultivated with Agrobacterium tumefaciens carrying pBIBSa. The ginseng cell lines carrying HBsAg-S gene were obtained. Transgenic cells were checked for the presence of target gene using PCR and RT-PCR. Samples containing the target gene showed a clear band at the site of 700 bp by agarose electrophoresis analysis (Figs. 2, 3). Expression levels determined by ELISA showed maximum expression levels of 184 ng HBsAg/g FW and 0.009% of the total soluble protein. HBsAg in ginseng cells were located both on the membrane and in the nuclei (Fig. 4).
authors.affiliation: Changchun Institute of Biological Product, Changchun 130062, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Hai-Peng
authors.initials: HP
authors.lastname: Yu
authors.forename: Jun
authors.initials: J
authors.lastname: Sheng
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Liu
authors.forename: Zhi-Wu
authors.initials: ZW
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Wei-Jin
authors.initials: WJ
authors.lastname: Sheng
authors.forename: Zheng-Lun
authors.initials: ZL
authors.lastname: Gao
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Yao
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Cai
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000378:metabolism; D005894:Panax / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D006514:Hepatitis B Surface Antigens