[The study on the STAT3 labeled by GFP affecting the migration of BEL-7402 cell line].
Signal transducers and activators of transcription (STATs) are key DNA-binding proteins in JAK/STATs signal pathway. Aberrant expression and activation of STAT3 have been identified in many kinds of tumors. We report here that constitutive activation of STAT3 was present in BEL-7402 cells. We constructed the fusing genes of STAT3 (wild type/mutant) and GFP to study the function of constitutively activated STAT3 in BEL-7402 cells. By measuring the migration of the cells labeled by GFP-STAT3(WT/CYF), we proved that overexpression of STAT3(WT) could augment the migration of BEL-7402 cells, while STAT3(CYF) could decrease the migration.
Yang
Run Lin
RL
The Jiangsu Key Laboratory of Molecular Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210097, China.
Pan
Fei Yan
FY
Yuan
Jun
J
Guo
Shi Ying
SY
Zhang
Hong Xia
HX
Zhao
Dong Hong
DH
Li
Chao Jun
CJ
D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D015850:Interleukin-6; D050796:STAT3 Transcription Factor; D049452:Green Fluorescent Proteins