From assembly to virus particle budding: pertinence of the detergent resistant membranes.
Detergent resistant membranes (DRMs) are the site of assembly for a variety of viruses. Here, we make use of Sendai virus mutant proteins that are not packaged into virus particles to determine the involvement of this assembly for the virus particle production. We found that, in the context of an infection, (1) all the Sendai virus proteins associated in part with DRMs, (2) mutant HN and M proteins not packaged into virus particles were similarly part of this association, (3) after M protein suppression resulting in a significant reduction of virus production, the floatation profile of the other viral proteins was not altered and finally (4) cellular cholesterol depletion did not decrease the virus particle production, although it somehow reduced their virus infectivity. These results led us to conclude that the assembly complex found in DRM fractions does not constitute a direct precursor of virus particle budding.
Gosselin-Grenet
Anne-Sophie
AS
Department of Microbiology and Molecular Medicine, University of Geneva Medical School, CMU, 1 rue Michel-Servet, CH-1211 Geneva 4, Switzerland.
Mottet-Osman
Geneviève
G
Roux
Laurent
L
D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism / Q000821:virology*; D002784:Cholesterol / Q000172:deficiency / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology*; D004351:Drug Resistance; D029082:Sendai virus / Q000254:growth & development* / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism; D014771:Virion / Q000254:growth & development; D019065:Virus Assembly / Q000502:physiology*
D003902:Detergents; D014764:Viral Proteins; D002784:Cholesterol