Genome-wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice (Oryza sativa L.).
Tremendous efforts have been taken worldwide to develop genome-wide genetic stocks for rice functional genomic (FG) research since the rice genome was completely sequenced. To facilitate FG research of complex polygenic phenotypes in rice, we report the development of over 20,000 introgression lines (ILs) in three elite rice genetic backgrounds for a wide range of complex traits, including resistances/tolerances to many biotic and abiotic stresses, morpho-agronomic traits, physiological traits, etc., by selective introgression. ILs within each genetic background are phenotypically similar to their recurrent parent but each carries one or a few traits introgressed from a known donor. Together, these ILs contain a significant portion of loci affecting the selected complex phenotypes at which allelic diversity exists in the primary gene pool of rice. A forward genetics strategy was proposed and demonstrated with examples on how to use these ILs for large-scale FG research. Complementary to the genome-wide insertional mutants, these ILs opens a new way for highly efficient discovery, candidate gene identification and cloning of important QTLs for specific phenotypes based on convergent evidence from QTL position, expression profiling, functional and molecular diversity analyses of candidate genes, highlights the importance of genetic networks underlying complex phenotypes in rice that may ultimately lead to more complete understanding of the genetic and molecular bases of quantitative trait variation in rice.
Li
Zhi-Kang
ZK
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China. z.li@cgiar.org
Fu
Bin-Ying
BY
Gao
Yong-Ming
YM
Xu
Jian-Long
JL
Ali
J
J
Lafitte
H R
HR
Jiang
Yun-Zhu
YZ
Rey
J Domingo
JD
Vijayakumar
C H M
CH
Maghirang
R
R
Zheng
Tian-Qing
TQ
Zhu
Ling-Hua
LH
D000222:Adaptation, Physiological / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods; D032461:Chromosomes, Plant / Q000235:genetics; D004190:Disasters; D018745:Genome, Plant; D006824:Hybridization, Genetic; D007178:Inbreeding; D015810:Linkage Disequilibrium; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D040641:Quantitative Trait Loci / Q000235:genetics