Interleukin 12 mediated prevention of tumorigenicity in murine cell lines derived from CD40L transgenic mice.
Cells derived from superficial and deep lymph nodes of transgenic mice in which CD40L expression was deregulated were grown in vitro. After 3 months of interleukin 3 or interleukin 12 stimulation, the cells remained interleukin-independent, showed the same in vitro growth characteristics, but LIL3+ cells were tumorigenic when reinoculated in vivo in nude mice, whereas interleukin-12-treated cells did not induce tumors. Our cell lines could provide a useful model to study the perturbation of the homeostasis allowing us to elucidate the role of cytokines as modulators of differentiation in the lymphoproliferative disorders.
Gribaldo
Laura
L
Laboratory of Hematotoxicology, ECVAM, Institute for Health and Consumer Protection, J.R.C., 21020 Ispra, Italy. laura.gribaldo@jrc.it
Malerba
Ilaria
I
Diodovich
Cristina
C
Sacco
Maria Grazia
MG
Collotta
Angelo
A
Eletti
Brighitta
B
Bassi
Loredana
L
D000818:Animals; D019013:CD40 Antigens / Q000378:metabolism; D023201:CD40 Ligand / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000276:immunology* / Q000473:pathology; D053222:Fas Ligand Protein; D018664:Interleukin-12 / Q000494:pharmacology / Q000502:physiology*; D007377:Interleukin-3 / Q000494:pharmacology / Q000502:physiology*; D007621:Karyotyping; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048069:Tumor Necrosis Factors / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism
D019013:CD40 Antigens; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D007377:Interleukin-3; D008562:Membrane Glycoproteins; D019253:Proto-Oncogene Proteins c-bcl-2; D048069:Tumor Necrosis Factors; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor; D023201:CD40 Ligand; D018664:Interleukin-12