Method for identification of mutant glutathione S-transferases conferring enhanced resistance to the anti-cancer drug chlorambucil.
We screened library of mutant glutathione S-transferases (GSTs) in Escherichia coli by successive treatments with anti-cancer drug chlorambucil and identified mutant GSTs that conferred enhanced resistance to host against chlorambucil compared with wild-type GST. This study provides a method to develop enzymes with improved efficiency of detoxification against cytotoxic substances.
Hirota
Kiyonori
K
Molecular and Cell Biology, National Institute ofAdvanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Hanyu
Yoshiro
Y