title: [Role of tissue factor in lung metastasis of colorectal carcinoma cells in vivo].
abstract: OBJECTIVE
  To investigate the role of tissue factor(TF) in hematogenous metastasis of human colorectal carcinoma cells (LOVO) in vivo.
  
  
  METHODS
  The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFc DNA were transfected into LOVO cells by lipofectamine 2000. TF protein expression in the transfected cells was detected by Western blot. Eighteen nude mice (Babl/c nu/nu) were randomly divided into three groups, and then transfected and untransfected LOVO cells were implanted via tail vein respectively. The nude mice were sacrificed 8 weeks after implantation, and the number of metastatic nodules in the lung was used to assess the metastatic ability of LOVO cells.
  
  
  RESULTS
  Compared with the untransfected group, TF expression of LOVO cells and the numbers of metastatic nodules in the lung increased in sense-TF cDNA transfection group (P< 0.05, P< 0.01, respectively), whereas decreased in antisense-TF cDNA transfection group (P< 0.05, P< 0.01, respectively).
  
  
  CONCLUSIONS
  TF can increase the hematogenous metastatic ability of human colorectal carcinoma cells (LOVO) in vivo.
authors.affiliation: Department of General Surgery, The First Hospital of Peking University, Beijing 100034, China. ronglong8@sina.com
authors.forename: Long
authors.initials: L
authors.lastname: Rong
authors.forename: Yuan-lian
authors.initials: YL
authors.lastname: Wan
authors.forename: Yu-cun
authors.initials: YC
authors.lastname: Liu
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Yao
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Yi-sheng
authors.initials: YS
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D013925:Thromboplastin