Characterization of petroleum-degrading bacteria from oil-contaminated sites in Vietnam.
Four petroleum-degrading bacterial strains, 2TN-NB, 6TBX-CL, MVK2-5, and XCK, were isolated from various oil-contaminated sites in Vietnam. Determination of the nucleotide sequence of the gene encoding 16S rRNA allowed 2TN-NB to be identified as Acinetobacter sp. and the other three stains as Pseudomonas sp. Among the four isolates, 2TN-NB was most effective in degrading crude oil: in 1 d, it degraded 95% of the crude oil in the culture medium (5%, v/v). The isolated strains could also degrade a sulfur-containing aromatic hydrocarbon, dibenzothiophene (DBT), with low efficiency. Except for MVK2-5, which degraded crude oil least efficiently, the isolates produced biosurfactants in amounts sufficient for structural analysis. FT-IR measurement suggested that strains 6TBX-CL and XCK produced glycolipid-type biosurfactants while that produced by 2TN-NB was of the polysaccharide type.
Huy
N Q
NQ
Institute of Biotechnology, Nghia Do, Cau Giay-Hanoi, Vietnam.
Jin
S
S
Amada
K
K
Haruki
M
M
Huu
N B
NB
Hang
D T
DT
Ha
D T
DT
Imanaka
T
T
Morikawa
M
M
Kanaya
S
S