The effects of metal ions on the structure and stability of the DNA gyrase B protein.
The effects of mono- and divalent metal ions on the DNA gyrase B subunit, on its 43 kDa and 47 kDa domains, and on two mutants in the Toprim domain (D498A and D500C) were investigated by means of circular dichroism and protein melting experiments. Both types of metal ion, with the notable exception of Mn2+, did not affect the conformational properties of the enzyme subunit at room temperature, but were able to produce selective and differential effects on protein stability. In particular, monovalent (K+) ions increased the stability of the gyrase B structure, whereas destabilising effects were most prominent using Mn2+ as the metal ion. Ca2+ and Mg2+ produced comparable changes in the gyrase B melting profile. Additionally, we found that monovalent (K+) ions were more effective in the 43 kDa N-terminal domain where ATP binding occurs, whereas divalent ions caused large modifications in the conformational stability of the 47 kDa C-terminal domain. Our results on gyrase B mutants indicate that D498 interacts with Mn2+, whereas it has little effect on the binding of the other ions tested. A D500C mutation, in contrast, effectively impairs Mg2+ affinity, suggesting effective contacts between this ion and D500 in the wild-type enzyme. Hence, the sites of metal ion complexation within the Toprim domain are modulated by the nature of the ion species. These results suggest a double role played by metal ions in the catalytic steps involving DNA gyrase B. One has to do with direct involvement of cations complexed to the Toprim domain in the DNA cutting-rejoining process, the other, until now overlooked, is connected to the dramatic changes in protein flexibility produced by ion binding, which reduces the energy required for the huge conformational changes essential for the catalytic cycle to occur.
Sissi
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C
Department of Pharmaceutical Sciences, University of Padova, Via Marzolo, 5, 35131 Padova, Italy.
Marangon
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Chemello
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Noble
C G
CG
Maxwell
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A
Palumbo
M
M
D001665:Binding Sites; D002384:Catalysis; D002413:Cations, Divalent / Q000494:pharmacology; D002414:Cations, Monovalent / Q000494:pharmacology; D002942:Circular Dichroism; D027081:DNA Gyrase / Q000737:chemistry* / Q000235:genetics; D004795:Enzyme Stability / Q000187:drug effects; D029968:Escherichia coli Proteins / Q000737:chemistry; D006358:Hot Temperature; D008670:Metals / Q000494:pharmacology*; D020125:Mutation, Missense; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation; D013816:Thermodynamics
D002413:Cations, Divalent; D002414:Cations, Monovalent; D029968:Escherichia coli Proteins; D008670:Metals; D027081:DNA Gyrase