title: Rhinovirus-stabilizing activity of artificial VLDL-receptor variants defines a new mechanism for virus neutralization by soluble receptors.
abstract: Members of the low-density lipoprotein receptor family possess various numbers of ligand binding repeats that non-equally contribute to binding of minor group human rhinoviruses. Using an artificial concatemer of five copies of repeat 3 of the human very-low density lipoprotein receptor, we demonstrate protection of HRV2 against low-pH mediated uncoating and inhibition of penetration of an RNA-specific fluorescent dye into the intact virion. This indicates that the recombinant receptor inhibits viral breathing and irreversible conformational modifications of the capsid that precede RNA release, providing a new mechanism for rhinovirus neutralization by soluble receptor molecules.
authors.affiliation: Institute of Analytical Chemistry, University of Vienna, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Nicodemou
authors.forename: Martina
authors.initials: M
authors.lastname: Petsch
authors.forename: Tunde
authors.initials: T
authors.lastname: Konecsni
authors.forename: Leopold
authors.initials: L
authors.lastname: Kremser
authors.forename: Ernst
authors.initials: E
authors.lastname: Kenndler
authors.forename: José M
authors.initials: JM
authors.lastname: Casasnovas
authors.forename: Dieter
authors.initials: D
authors.lastname: Blaas
mesh_terms: D044966:Anti-Retroviral Agents / Q000737:chemistry / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D012367:RNA, Viral / Q000032:analysis / Q000187:drug effects*; D011973:Receptors, LDL / Q000737:chemistry* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*; D012229:Rhinovirus / Q000187:drug effects*; D014771:Virion / Q000737:chemistry / Q000187:drug effects
chemical_list: D044966:Anti-Retroviral Agents; D005456:Fluorescent Dyes; C506068:MBP-V33333 peptide; D012367:RNA, Viral; D011973:Receptors, LDL; D011993:Recombinant Fusion Proteins; C036053:VLDL receptor