title: Rapid homogeneous immunoassay of peptides based on bioluminescence resonance energy transfer from firefly luciferase.
abstract: A sensitive homogeneous immunoassay is needed in the field of clinical diagnostics. Here we propose a rapid and potentially sensitive homogeneous immunoassay of peptide epitope based on the bioluminescence resonance energy transfer (BRET). A GST peptide tag fused to the N-terminus of firefly luciferase, and Cy3 or Cy3.5-labeled anti-peptide antibody were prepared to detect BRET from the hybrid luciferase to the fluorolabeled antibody. By measuring the spectral change due to BRET, the amount of c-myc peptide was successfully determined in a short period.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Yamakawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kitayama
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nagamune