title: Association between large-scale genomic homozygosity without chromosomal loss and nonseminomatous germ cell tumor development.
abstract: The genotype of a tumor determines its biology and clinical behavior. The genetic alterations associated with the unique embryonal morphology of nonseminomatous subtypes of testicular germ cell tumors remain to be established. Using single nucleotide polymorphism microarray analysis, we found in all of the 15 nonseminomas analyzed, large-scale chromosomal homozygosities, most of which were not associated with relative chromosome loss. This unusual genotype, distinguishing nonseminoma from seminomas and other human tumors, may be associated with the special embryonal development morphologic transition of this malignancy. Based on these genetic data, we hypothesized a new potential origin of nonseminomas through sperm fusion. Nonrandom involvement of certain chromosomes also suggests that genes on these chromosome regions may play an important role in nonseminoma development.
authors.affiliation: Department of Medical Oncology, Barts and London School of Medicine and Dentistry, Queen Mary, University of London, London, United Kingdom. yong.lu@cancer.org.uk
authors.forename: Yong-Jie
authors.initials: YJ
authors.lastname: Lu
authors.forename: Jinshu
authors.initials: J
authors.lastname: Yang
authors.forename: Elodie
authors.initials: E
authors.lastname: Noel
authors.forename: Spyros
authors.initials: S
authors.lastname: Skoulakis
authors.forename: Tracy
authors.initials: T
authors.lastname: Chaplin
authors.forename: Manoj
authors.initials: M
authors.lastname: Raghavan
authors.forename: Trisha
authors.initials: T
authors.lastname: Purkis
authors.forename: Alan
authors.initials: A
authors.lastname: McIntyre
authors.forename: Sakunthala C
authors.initials: SC
authors.lastname: Kudahetti
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Naase
authors.forename: Dan
authors.initials: D
authors.lastname: Berney
authors.forename: Janet
authors.initials: J
authors.lastname: Shipley
authors.forename: R Timothy D
authors.initials: RT
authors.lastname: Oliver
authors.forename: Bryan D
authors.initials: BD
authors.lastname: Young
mesh_terms: D002872:Chromosome Deletion; D005838:Genotype; D006720:Homozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009373:Neoplasms, Germ Cell and Embryonal / Q000235:genetics*; D011003:Ploidies; D020641:Polymorphism, Single Nucleotide; D013736:Testicular Neoplasms / Q000235:genetics*