[Preliminary analysis of retinal gene expression profile of diabetic rat].
OBJECTIVE
Establishing the retinal gene expression profiles of non-diabetic rat and diabetic rat and comparing the profiles in order to analyze the possible genes related with diabetic retinopathy.


METHODS
The whole retinal transcriptional fragments of non-diabetic rat and 8-week diabetic rat were obtained by restriction fragments differential display-PCR (RFDD-PCR). Bioinformatic analysis of retinal gene expression was performed using soft wares, including Fragment Analysis. After comparison of the expression profiles, the related gene fragments of diabetic retinopathy were initially selected as the target gene of further approach.


RESULTS
A total of 3639 significant fragments were obtained. By means of more than 3-fold contrast of fluorescent intensity as the differential expression standard, the authors got 840 differential fragments, accounting for 23.08% of the expressed numbers and including 5 visual related genes, 13 excitatory neruotransmitter genes and 3 inhibitory neurotransmitter genes. At the 8th week, the expression of Rhodopsin kinase, beta-arrestin, Phosducinìrod photoreceptor cGMP-gated channel and Rpe65 as well as iGlu R1-4 were down-regulated. mGluRs and GABA-Rs were all up-regulated, whereas the expression of GlyR was unchanged.


CONCLUSIONS
These results prompt again that the changes in retinal nervous layer of rat have occurred at an early stage of diabetes. The genes expression pattern of visual related genes and excitatory and inhibitory neurotransmitters in rat diabetic retina have been involved in neuro-dysfunctions of diabetic retina.
Mei
Yan
Y
Department of Anatomy, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, PR China. meikm@163.com
Zhou
Hong-ying
HY
Xiang
Tao
T
Lu
You-guang
YG
Li
Ai-dong
AD
Tang
En-jie
EJ
Yang
Hui-jun
HJ
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000235:genetics*; D003930:Diabetic Retinopathy / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods