Dietary effects of copper and iron deficiency on rat intestine: a differential display proteome analysis.
Copper and iron are cofactors of many metallo-proteins that accomplish vital functions, such as oxygen and electron transport. Specific metabolic pathways have been selected through evolution, although still not fully elucidated, to confine the dangerous reactivity of their free ionic forms. Inadequate supply of both metals can severely affect basic physiological functions. A differential analysis of the rat intestinal proteome evidenced the following dietary copper- and iron-deficiencies, i.e., significant changes in the levels of proteins belonging to different functional classes (glucose and fatty acid metabolism, molecular chaperones, cytoskeleton plasticity, vitamin transporters). The presented results bring new perspectives to understand the role of copper and iron in the metabolic pathways and provide novel diagnostic tools to characterize the effects of subclinical deficiencies of both metals in unbalanced nutritional disorders.
Tosco
Alessandra
A
Dipartimento di Scienze Farmaceutiche Università degli Studi di Salerno, Via Ponte Don Melillo, 84084 Fisciano (SA), Italy.
Siciliano
Rosa Anna
RA
Cacace
Giuseppina
G
Mazzeo
Maria Fiorella
MF
Capone
Roberta
R
Malorni
Antonio
A
Leone
Arturo
A
Marzullo
Liberato
L
D000818:Animals; D003285:Contractile Proteins / Q000737:chemistry; D003300:Copper / Q000737:chemistry / Q000172:deficiency*; D004583:Electrons; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000378:metabolism; D064448:Filamins; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007413:Intestinal Mucosa / Q000378:metabolism*; D007477:Ions; D007501:Iron / Q000737:chemistry; D000090463:Iron Deficiencies; D008297:Male; D013058:Mass Spectrometry; D008840:Microfilament Proteins / Q000737:chemistry; D008954:Models, Biological; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry; D010751:Phosphopyruvate Hydratase / Q000737:chemistry; D017510:Protein Folding; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D014809:Vitamin D-Binding Protein / Q000737:chemistry
D003285:Contractile Proteins; D005227:Fatty Acids; D064448:Filamins; D007477:Ions; D008840:Microfilament Proteins; D018832:Molecular Chaperones; D014809:Vitamin D-Binding Protein; D003300:Copper; D007501:Iron; D010751:Phosphopyruvate Hydratase; D010100:Oxygen