title: Inhibition of toxicogenic Bacillus cereus in rice-based foods by enterocin AS-48.
abstract: The antimicrobial effect of the broad-spectrum bacteriocin enterocin AS-48 against the toxicogenic psychrotrophic strain Bacillus cereus LWL1 has been investigated in a model food system consisting of boiled rice and in a commercial infant rice-based gruel dissolved in whole milk stored at temperatures of 37 degrees C, 15 degrees C and 6 degrees C. In food samples supplemented with enterocin AS-48 (in a concentration range of 20-35 mug/ml), viable cell counts decreased rapidly over incubation time, depending on the bacteriocin concentration, the temperature of incubation and the food sample. Enterotoxin production at 37 degrees C was also inhibited. Heat sensitivity of endospores increased markedly in food samples supplemented with enterocin AS-48: inactivation of endospores was achieved by heating for 1 min at 90 degrees C in boiled rice or at 95 degrees C in rice-based gruel. Activity of enterocin AS-48 in rice gruel was potentiated by sodium lactate in a concentration-dependent way.
authors.affiliation: Area de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, Campus Las Lagunillas s/n, 23071-Jaén, Spain.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Grande
authors.forename: Rosario
authors.initials: R
authors.lastname: Lucas
authors.forename: Hikmate
authors.initials: H
authors.lastname: Abriouel
authors.forename: Eva
authors.initials: E
authors.lastname: Valdivia
authors.forename: Nabil Ben
authors.initials: NB
authors.lastname: Omar
authors.forename: Mercedes
authors.initials: M
authors.lastname: Maqueda
authors.forename: Manuel
authors.initials: M
authors.lastname: Martínez-Bueno
authors.forename: Magdalena
authors.initials: M
authors.lastname: Martínez-Cañamero
authors.forename: Antonio
authors.initials: A
authors.lastname: Gálvez
mesh_terms: D001409:Bacillus cereus / Q000187:drug effects* / Q000254:growth & development; D001430:Bacteriocins / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D005511:Food Handling / Q000379:methods*; D006801:Humans; D007223:Infant; D007225:Infant Food; D007231:Infant, Newborn; D012275:Oryza / Q000382:microbiology*; D013696:Temperature; D013997:Time Factors
chemical_list: D001430:Bacteriocins; C107799:enterocin A; C466236:enterocin AS-48, Enterococcus faecalis