title: 5' Untranslated region of the HSP18.2 gene contributes to efficient translation in plant cells.
abstract: Maximizing the amount of protein translated per unit mRNA is an important goal in establishing expression systems. The 5' untranslated region (5'-UTR) of mRNA is known to play an important role in determining the rate of translation. The full length 5'-UTR from the Arabidopsis thaliana heat shock protein (HSP) gene,HSPI8.2 gene, was inserted into the cloning site between the cauliflower mosaic virus 35S RNA promoter and beta-glucuronidase (GUS) gene in the pBI221 plasmid. When this construct was transfected into Nicotiana tabacum (tobacco) BY-2 protoplasts, the level of protein was about 10-fold higher than that of unmodified pB1221. The accumulation of each transcript was the same level. We also demonstrated that the 5'-UTR of the HSP18.2 gene enhances the rate of translation in stable transgenic BY-2 clones and Arabidopsis T87 protoplasts. The 5'-UTRs of the other Arabidopsis HSP genes -HSP17.4, HSP81-1,HSP81-2, andHSP81-3 - also conferred efficient translation. These 5'-UTRs ofHSP genes may be of use in increasing the expression of foreign proteins. In combination with a strong promoter, it can be used in the development of efficient protein production systems.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Dansako
authors.forename: Ko
authors.initials: K
authors.lastname: Kato
authors.forename: Junko
authors.initials: J
authors.lastname: Satoh
authors.forename: Masami
authors.initials: M
authors.lastname: Sekine
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yoshida
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Shinmyo