title: Endocytosis of HIV-1 activates plasmacytoid dendritic cells via Toll-like receptor-viral RNA interactions.
abstract: HIV-1 directly activates human plasmacytoid DCs (pDCs) by upregulating the expression of costimulatory and MHC molecules and maturation markers, increasing T cell stimulatory activity, and inducing the production of type I interferons and TNF-alpha. A consequence of this activation is the bystander maturation of myeloid DCs and overall enhancement of antigen-presenting function. However, little is known about the mechanism(s) of pDC activation by HIV-1. Here we demonstrate by in vitro studies that IFN-alpha production by pDC in response to HIV-1 requires at least 2 interactions between the cell and virus. Initially, envelope-CD4 interactions mediate endocytosis of HIV-1, as demonstrated through the use of inhibitors of binding, fusion, endocytosis, and endosomal acidification. Subsequently, endosomally delivered viral nucleic acids, particularly RNA, stimulate pDCs through TLRs, as activation is reproduced with purified genomic RNA but not viral RNA packaging-deficient HIV-1 and blocked with different inhibitory TLR ligands. Finally, by using genetic complementation, we show that TLR7 is the likely primary target. Viral RNA rather than DNA in early retrotranscripts appears to be the active factor in HIV-1 that induces IFN-alpha secretion by pDCs. Since the decline in pDCs in chronic HIV-1 infection is associated with high viral loads and opportunistic infections, exploiting this natural adjuvant activity of HIV-1 RNA might be useful in the development of vaccines for the prevention of AIDS.
authors.affiliation: New York University School of Medicine, New York, New York 10016, USA.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Beignon
authors.forename: Kelli
authors.initials: K
authors.lastname: McKenna
authors.forename: Mojca
authors.initials: M
authors.lastname: Skoberne
authors.forename: Olivier
authors.initials: O
authors.lastname: Manches
authors.forename: Ida
authors.initials: I
authors.lastname: DaSilva
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Kavanagh
authors.forename: Marie
authors.initials: M
authors.lastname: Larsson
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Gorelick
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Lifson
authors.forename: Nina
authors.initials: N
authors.lastname: Bhardwaj
mesh_terms: D015704:CD4 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D004705:Endocytosis / Q000276:immunology*; D019302:HIV Envelope Protein gp160 / Q000378:metabolism; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D012367:RNA, Viral / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000378:metabolism*
chemical_list: D015704:CD4 Antigens; D019302:HIV Envelope Protein gp160; D012367:RNA, Viral; D051193:Toll-Like Receptors