title: P-selectin glycoprotein ligand-1 (PSGL-1) is up-regulated on leucocytes from patients with chronic obstructive pulmonary disease.
abstract: Chronic obstructive pulmonary disease (COPD) is characterized by a dysregulated recruitment of circulating leucocytes into the lung which is associated with the onset and progress of the disease. P-selectin glycoprotein ligand-1 (PSGL-1) is expressed on leucocytes and plays an essential role in primary leucocyte-endothelial cell adhesive contacts. The present study investigated if PSGL-1 is up-regulated on leucocytes of COPD patients. Peripheral blood samples were collected from COPD patients as well as controls (smoking, nonsmoking volunteers) and subjected to analysis of PSGL-1 expression on leucocytes, i.e. neutrophils, eosinophils, monocytes and lymphocytes by flow cytometry. No significant difference was observed between healthy nonsmoking and healthy smoking control subjects. In contrast, PSGL-1 expression was found to be significantly increased on the surface of all four leucocyte populations in COPD patients compared to both control groups. The finding that PSGL-1 surface expression is up-regulated on leucocytes of COPD patients as compared to leucocytes of controls suggests PSGL-1 as a potential target for anti-inflammatory treatment.
authors.affiliation: Division of Pulmology, Department of Cardiology, Pulmology, Angiology, Charite-Universitätsmedizin Berlin, Germany. axel.schumacher@charite.de
authors.forename: A
authors.initials: A
authors.lastname: Schumacher
authors.forename: U
authors.initials: U
authors.lastname: Liebers
authors.forename: M
authors.initials: M
authors.lastname: John
authors.forename: V
authors.initials: V
authors.lastname: Gerl
authors.forename: M
authors.initials: M
authors.lastname: Meyer
authors.forename: C
authors.initials: C
authors.lastname: Witt
authors.forename: G
authors.initials: G
authors.lastname: Wolff
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000378:metabolism*; D008024:Ligands; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood*; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000276:immunology; D012907:Smoking / Q000097:blood; D015854:Up-Regulation
chemical_list: D008024:Ligands; D008562:Membrane Glycoproteins; D019007:P-Selectin; C084882:P-selectin ligand protein