Saccharomyces boulardii inhibits water and electrolytes changes induced by castor oil in the rat colon.
The biotherapeutic agent Saccharomyces boulardii has been shown to inhibit castor oil-induced diarrhoea in rats. The present study investigated the mechanism(s) of this antidiarrhoeal effect in terms of water and electrolyte (sodium, potassium and chloride) changes using two rat models. A single oral dose of S. boulardii of up to 12 x 10(10) CFU/kg of viable cells did not inhibit castor oil-induced fluid secretion in the enteropooling model. However, the yeast dose dependently reduced castor oil induced fluid secretion into the colon, with a significant protection at 12 x 10(10) CFU/kg. In this model, castor oil reversed net sodium and chloride absorption into net secretion, and increased net potassium secretion into the lumen. Single pre-treatment with S. boulardii at 4 and 12 x 10(10) CFU/kg dose dependently decreased these electrolyte changes. In conclusion, S. boulardii possesses potent anti-secretory properties versus water and electrolyte secretion induced by castor oil in the rat colon.
Girard
Philippe
P
Biocodex, Service de Pharmacologie, Zac de Mercières, 60200, Compiègne, France. p.girard@biocodex.fr
Pansart
Yannick
Y
Coppe
Marie-Claude
MC
Gillardin
Jean-Marie
JM
D000818:Animals; D000930:Antidiarrheals / Q000494:pharmacology; D001285:Atropine / Q000494:pharmacology; D002368:Castor Oil / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology*; D003967:Diarrhea / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control; D004573:Electrolytes / Q000378:metabolism*; D008139:Loperamide / Q000494:pharmacology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012440:Saccharomyces / Q000502:physiology*; D014867:Water / Q000378:metabolism*
D000930:Antidiarrheals; D004573:Electrolytes; D014867:Water; D008139:Loperamide; D001285:Atropine; D002368:Castor Oil