Selection and characterization of phage miniantibodies to actins of different origin.
Single-chain miniantibodies (scFv's) to actin were obtained by the phage display method. A naive combinatorial phage display library of murine scFv's (containing 2x10(8) independent recombinant clones) was used to select miniantibodies. After three rounds of selection two clones producing miniantibodies to chicken smooth muscle actin with affinity constants of 1.4x10(7) and 1.2x10(6) M(-1) were chosen. The isolated miniantibodies could specifically detect various plant and animal actins.
Kostesha
N V
NV
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, 410049, Russia.
Laman
A G
AG
Shepelyakovskaya
A O
AO
Zaitseva
I S
IS
Orlov
V P
VP
Dykman
L A
LA
Brovko
F A
FA
Sokolov
O I
OI
D000199:Actins / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D019151:Peptide Library; D010940:Plant Proteins / Q000276:immunology; D011817:Rabbits; D013045:Species Specificity; D031307:Vicia faba / Q000378:metabolism
D000199:Actins; D000911:Antibodies, Monoclonal; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D010940:Plant Proteins; C055966:immunoglobulin Fv