title: Incorporation of spin-labelled amino acids into proteins.
abstract: The elucidation of structure and function of proteins and membrane proteins by EPR spectroscopy has become increasingly important in recent years as technological advances have been made in the design of spectrometers and in the chemistry of the nitroxide group. These new developments have increased the demand for tailor-made amino acids carrying a spin label on the one hand and for reliable methods for their incorporation into proteins on the other. Here we describe methods for site-specific spin labelling of proteins. It is shown that a combination of recombinant synthesis of proteins with chemically produced peptides (expressed protein ligation) allows the preparation of site-specifically spin-labelled proteins.
authors.affiliation: Max-Planck-Institut für Molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany.
authors.forename: Christian F W
authors.initials: CF
authors.lastname: Becker
authors.forename: Kester
authors.initials: K
authors.lastname: Lausecker
authors.forename: Mária
authors.initials: M
authors.lastname: Balog
authors.forename: Tamás
authors.initials: T
authors.lastname: Kálai
authors.forename: Kálmán
authors.initials: K
authors.lastname: Hideg
authors.forename: Heinz-Jürgen
authors.initials: HJ
authors.lastname: Steinhoff
authors.forename: Martin
authors.initials: M
authors.lastname: Engelhard
mesh_terms: D000596:Amino Acids / Q000138:chemical synthesis / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D015394:Molecular Structure; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D000596:Amino Acids; D011506:Proteins; D013113:Spin Labels