Construction of a combinatorial protein library displayed on yeast cell surface using DNA random priming method.
The random DNA fragments, generated from mRNAs of Saccharomyces cerevisiae using a novel "DNA random priming method", were inserted into a surface display vector to construct a combinatorial random protein library. Its surface display was confirmed by immunofluorescence labeling of the RGS (His)6 reporter inserted, and its potential was also evaluated.
Zou
W
W
Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.
Ueda
M
M
Yamanaka
H
H
Tanaka
A
A