title: Enzymatic synthesis of novel disaccharides using disaccharide phosphorylases.
abstract: A method for the synthesis of novel disaccharides was developed. It involved the following two steps. The first step consisted of two continuous reactions: the conversion of maltose to beta-D-glucose-1-phosphate and D-glucose by the phosphorolytic activity of maltose phosphorylase and the specific consumption of only D-glucose by incubation with glucose-consuming yeast cells. The second step involved the addition of an appropriate carbohydrate and its condensation with the remaining beta-D-glucose-1-phosphate by the synthetic activity of maltose phosphorylase or trehalose phosphorylase. Several factors affecting the yields of disaccharides were optimized. Using this method, five maltose-like derivatives and two trehalose-like derivatives were synthesized from maltose and the corresponding carbohydrates. Among these, 4-O-alpha-D-glucopyranosyl-L-fucopyranose (Glc(alpha1-4)Fuc) and alpha-d-glucopyranosyl alpha-D-fucopyranoside (Glc(alpha1-1alpha)Fuc) were purified, and identified by 1H NMR and 13C NMR.
authors.affiliation: Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machida-shi, Tokyo 194-8533, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Aisaka
authors.forename: T
authors.initials: T
authors.lastname: Masuda-Kato
authors.forename: T
authors.initials: T
authors.lastname: Chikamune
authors.forename: K
authors.initials: K
authors.lastname: Kamitori
authors.forename: Y
authors.initials: Y
authors.lastname: Uosaki
authors.forename: Y
authors.initials: Y
authors.lastname: Saito