Response regulator DegU of Listeria monocytogenes regulates the expression of flagella-specific genes.
An isogenic mutant of Listeria monocytogenes EGD with a deletion of the response regulator gene degU showed a lack of motility due to the absence of flagella. In the present study, we used two-dimensional gel electrophoresis, mass-spectrometry and microarray analyses to identify the listerial genes that depend on DegU for expression. We found that the two L. monocytogenes operons encoding flagella-specific genes and the monocistronically transcribed flaA gene are positively regulated by DegU at 24 degrees C, but are not expressed at 37 degrees C.
Williams
Tatjana
T
Lehrstuhl für Mikrobiologie, Theodor Boveri-Institut für Biowissenschaften der Universität Würzburg, Germany.
Joseph
Biju
B
Beier
Dagmar
D
Goebel
Werner
W
Kuhn
Michael
M
D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005407:Flagella / Q000235:genetics*; D005408:Flagellin / Q000235:genetics; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008089:Listeria monocytogenes / Q000235:genetics*; D013058:Mass Spectrometry; D020411:Oligonucleotide Array Sequence Analysis; D009876:Operon; D020543:Proteome / Q000032:analysis; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D013696:Temperature
D001426:Bacterial Proteins; C066497:DegU protein, Bacteria; D020543:Proteome; D012329:RNA, Bacterial; D012333:RNA, Messenger; D005408:Flagellin; C067138:flaA protein, bacteria