title: An efficient and versatile solid-phase synthesis of 5'- and 3'-conjugated oligonucleotides.
abstract: [reaction: see text] An easy and efficient solid-phase strategy to obtain 5'- and 3'-oligonucleotide conjugates in highly pure form has been developed. Ad hoc derivatized solid supports, to which the first nucleoside unit can be attached through a phosphate linkage, have been exploited both in a pre- and post-DNA assembly conjugation approach. A number of 5'- or 3'- oligonucleotide conjugates, incorporating a variety of labels covalently linked through a phosphodiester or a phosphoramidate bond, have been synthesized and characterized.
authors.affiliation: Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico II, Complesso Universitario di Monte S. Angelo, Italy.
authors.forename: Jennifer
authors.initials: J
authors.lastname: D'Onofrio
authors.forename: Daniela
authors.initials: D
authors.lastname: Montesarchio
authors.forename: Lorenzo
authors.initials: L
authors.lastname: De Napoli
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Fabio
mesh_terms: D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite