Search for a two-input model for future investigations of 'synaptic tagging' in freely moving animals in vivo.
Processes of "synaptic tagging" guarantee synaptic input specificity after the induction of a protein synthesis-dependent late long-term potentiation (late-LTP). Distinct high-frequency stimulation can set a transient "synaptic tag" at the activated synapses, which captures plasticity-related proteins (PRPs) synthesized synapse-non-specifically in dendritic branches/compartments or the somata. Thus, only those synapses, which expressed a "tag", are also able to express late-LTP. Additionally, it was shown that the synthesis of PRPs is triggered by heterosynaptic, non-glutamatergic requirements during LTP-induction in tissue from adult animals. All these experiments were performed in hippocampal slices in vitro so far. Two questions now arise: first, is it possible to describe processes of 'synaptic tagging' in the intact, freely moving animal and second, is the stimulation of glutamatergic inputs sufficient to induce 'tagging' or is the co-activation of a modulatory-heterosynaptic input, also required for the process? We have first developed a technique, which allows us now to induce distinct forms of LTP at the ipsilateral CA1 site by specifically stimulating glutamatergic hippocampal structures at the contralateral site in the intact, freely moving rat. Thus, the used stimulation protocol allowed us to activate two separate synaptic inputs to the same neuronal stimulation, a pre-requisite for tagging-experiments to be investigated in vivo.
Hassan
Hadir
H
Department of Neurophysiology, Leibniz-Institute for Neurobiology, Brenneckestr. 6, 39118 Magdeburg, Germany.
Frey
Sabine
S
Frey
Julietta U
JU
D000771:Anesthesia, Intravenous; D018686:Anesthetics, Intravenous; D000818:Animals; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D004594:Electrophysiology; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D007839:Functional Laterality / Q000502:physiology; D017774:Long-Term Potentiation / Q000187:drug effects; D008297:Male; D008959:Models, Neurological; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000502:physiology*; D014520:Urethane
D018686:Anesthetics, Intravenous; D014520:Urethane