Isolation and genetic determination of a Saccharomyces cerevisiae mutant that produces an endo-polygalacturonase.
A Saccharomyces cerevisiae mutant that produces an endo-polygalacturonase (PGase) was isolated. The PGase gene was revealed to be located on chromosome X in both the mutant and its parental strain. The 5'-upstream region of the PGase gene in the mutant was entirely identical with that of its parent. Crossing of the mutant with a PGase-negative strain showed that the phenotype of PGase production was recessive. These results suggest that the mutant is rendered defective in a transacting factor that normally represses the expression of the PGase gene.
Hirose
N
N
Department of Applied Biochemistry, College of Agriculture, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531 Japan.
Fujii
M
M
Kishida
M
M
Kawasaki
H
H
Sakai
T
T