Biodegradation of long-chain n-paraffins from waste oil of car engine by Acinetobacter sp.
Microorganisms that degrade long-chain n-paraffins from used car engine oil were isolated from soil. For the screening, a fraction of n-paraffin prepared from car engine oil was applied as the sole carbon source. The strain was identified as Acinetobacter sp. The ability of the strain to assimilate long-chain n-paraffins was assessed and characterized. The strain mineralized long-chain n-paraffins (0.1% w/v) in the minimal medium after cultivation for 96 h and also reduced the weight of the waste oil added (1% w/v) by 20% after 72 h without an extracellular biosurfactant. When n-hexadecane was fed as substrate, 1-hexadecanol and 1-hexadecanoic acid were detected as the intermediates by gas chromatography/mass spectrometry. This indicates that the long-chain n-paraffins were metabolized via the terminal oxidation pathway of n-alkane.
Koma
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D
Department of Bioscience & Technology, Faculty of Science & Engineering, Ritsumeikan University, Nojihigashi, Kusatsu-shi, Shiga-ken 525-8577, Japan.
Hasumi
F
F
Yamamoto
E
E
Ohta
T
T
Chung
S Y
SY
Kubo
M
M