title: Interleukin 12 mediated prevention of tumorigenicity in murine cell lines derived from CD40L transgenic mice.
abstract: Cells derived from superficial and deep lymph nodes of transgenic mice in which CD40L expression was deregulated were grown in vitro. After 3 months of interleukin 3 or interleukin 12 stimulation, the cells remained interleukin-independent, showed the same in vitro growth characteristics, but LIL3+ cells were tumorigenic when reinoculated in vivo in nude mice, whereas interleukin-12-treated cells did not induce tumors. Our cell lines could provide a useful model to study the perturbation of the homeostasis allowing us to elucidate the role of cytokines as modulators of differentiation in the lymphoproliferative disorders.
authors.affiliation: Laboratory of Hematotoxicology, ECVAM, Institute for Health and Consumer Protection, J.R.C., 21020 Ispra, Italy. laura.gribaldo@jrc.it
authors.forename: Laura
authors.initials: L
authors.lastname: Gribaldo
authors.forename: Ilaria
authors.initials: I
authors.lastname: Malerba
authors.forename: Cristina
authors.initials: C
authors.lastname: Diodovich
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Sacco
authors.forename: Angelo
authors.initials: A
authors.lastname: Collotta
authors.forename: Brighitta
authors.initials: B
authors.lastname: Eletti
authors.forename: Loredana
authors.initials: L
authors.lastname: Bassi
mesh_terms: D000818:Animals; D019013:CD40 Antigens / Q000378:metabolism; D023201:CD40 Ligand / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000276:immunology* / Q000473:pathology; D053222:Fas Ligand Protein; D018664:Interleukin-12 / Q000494:pharmacology / Q000502:physiology*; D007377:Interleukin-3 / Q000494:pharmacology / Q000502:physiology*; D007621:Karyotyping; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048069:Tumor Necrosis Factors / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism
chemical_list: D019013:CD40 Antigens; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D007377:Interleukin-3; D008562:Membrane Glycoproteins; D019253:Proto-Oncogene Proteins c-bcl-2; D048069:Tumor Necrosis Factors; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor; D023201:CD40 Ligand; D018664:Interleukin-12