Promotion of monolayer formation and high expression of ammonia metabolism of primary rat hepatocytes on arginine-glycine-aspartic acid-containing peptide-coated polystyrene dish.
Cell adhesive peptide Arg-Gly-Asp (RGD) was immobilized using ProNectin F (PnF) on a nontreated polystyrene petri dish at a PnF density of 20 ng cm(-2), which is sufficient for primary rat hepatocyte immobilization. The density of PnF on the dish affects cell morphology and expression of the differentiated functions within the range of 2-2500 ng cm(-2). An optimal monolayer state with defined cell boundaries and hepatocyte nuclei was formed on a 2500 ng cm(-2) PnF-coated petri dish, and the ammonia metabolic function was expressed at as high a level as in the hepatocyte/spheroid. We conclude that 2500 ng cm(-2) PnF enhances the morphological stability and expression of liver-specific functions of the hepatocyte.
Ijima
Hiroyuki
H
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. ijima@chem-eng.kyushu-u.ac.jp
Kawakami
Koei
K
D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002449:Cell Aggregation / Q000187:drug effects / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005353:Fibronectins / Q000008:administration & dosage* / Q000737:chemistry; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009842:Oligopeptides / Q000008:administration & dosage* / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*
D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D009842:Oligopeptides; D011137:Polystyrenes; D011994:Recombinant Proteins; C070367:ProNectin F; D000641:Ammonia; C047981:arginyl-glycyl-aspartic acid