FHA domain-ligand interactions: importance of integrating chemical and biological approaches.
Combinatorial library screens based on binding affinity may preferentially select ligands with ability for ionic interactions and miss the biologically relevant ligands that bind more weakly with predominantly hydrophobic interactions.
Mahajan
Anjali
A
Biophysics Program, The Ohio State University, Columbus, Ohio 43210, USA.
Yuan
Chunhua
C
Pike
Brietta L
BL
Heierhorst
Jörg
J
Chang
Chi-Fon
CF
Tsai
Ming-Daw
MD
D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D010769:Phosphothreonine / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism
D018797:Cell Cycle Proteins; C464906:Fkh1 protein, S cerevisiae; D051858:Forkhead Transcription Factors; D008024:Ligands; D029701:Saccharomyces cerevisiae Proteins; D010769:Phosphothreonine