Efficient enzymatic synthesis of 4-methylumbelliferyl N-acetyllactosaminide and 4-methylumbelliferyl sialyl N-acetyllactosaminides employing beta-D-galactosidase and sialyltransferases.
4-Methylumbelliferyl N-acetyllactosaminide and 4-methylumbelliferyl sialyl N-acetyllactosaminides, which are used for the assay of sialytransferase, neuraminidase and fucosyltransferase, were synthesized, respectively, by the beta-D: -galactosidase from Bacillus circulans and by a recombinant rat alpha2,3-(N)-sialyltransferase or rat liver alpha2,6-(N)-sialyltransferase with CMP-N-acetylneuraminic acid as donor.
Zeng
Xiaoxiong
X
Department of Biotechnology, College of Food Science and Technology, Nanjing Agricultural University, 210095, Weigang, Nanjing, Jiangsu, PR China. zengxx@njau.edu.cn
Sun
Yi
Y
Uzawa
Hirotaka
H
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D000818:Animals; D001407:Bacillus / Q000201:enzymology; D006923:Hymecromone / Q000031:analogs & derivatives*; D007785:Lactose / Q000031:analogs & derivatives* / Q000096:biosynthesis; D008099:Liver / Q000201:enzymology; D051381:Rats; D012799:Sialyltransferases / Q000378:metabolism*; D014468:Umbelliferones / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000378:metabolism*
C507574:4-methylumbelliferyl sialyl N-acetyllactosaminide; D014468:Umbelliferones; C025158:4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside; D006923:Hymecromone; D012799:Sialyltransferases; D001616:beta-Galactosidase; D007785:Lactose; D000117:Acetylglucosamine