DADLE induces a reversible hibernation-like state in HeLa cells.
[D-Ala(2)-D-Leu(5)-Enkephalin] (DADLE) can induce hibernation when injected into ground squirrels in summer and is able to increase the survival time of explanted organs such as liver and lung. Since cell metabolism is a target of this peptide, we have treated HeLa cells with DADLE and investigated its possible effect on transcription and proliferation as well as the resumption of metabolic activity after treatment. The labelling for Pol I, Pol II and for splicing factors such as snRNPs and SC-35 decreased after treatment as did the nucleolar labelling for UBF. In treated cells, several spherical nuclear bodies were found to be labelled for hnRNPs. In parallel, the number of proliferating cells decreased after treatment with DADLE. After recovery, there was a gradual resumption of cell function: transcription and splicing factors had a distribution similar to that of controls; proliferation resumed; nuclear bodies, representing storage sites for RNPs, disappeared.
Vecchio
Lorella
L
Laboratorio di Biologia Cellulare e Neurobiologia, Dipartimento di Biologia Animale, Università di Pavia and Istituto di Genetica Molecolare del CNR, Piazza Botta 10, 27100, Pavia, Italy.
Soldani
Cristiana
C
Bottone
Maria Grazia
MG
Malatesta
Manuela
M
Martin
Terence E
TE
Rothblum
Lawrence I
LI
Pellicciari
Carlo
C
Biggiogera
Marco
M
D000963:Antimetabolites / Q000378:metabolism; D015153:Blotting, Western; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D016308:Enkephalin, Leucine-2-Alanine / Q000494:pharmacology*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D012334:RNA, Neoplasm / Q000096:biosynthesis; D020394:Vault Ribonucleoprotein Particles / Q000187:drug effects
D000963:Antimetabolites; D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D012334:RNA, Neoplasm; D020394:Vault Ribonucleoprotein Particles; D016308:Enkephalin, Leucine-2-Alanine; D001973:Bromodeoxyuridine; D016650:Fluorescein-5-isothiocyanate