title: Quencher-free, end-stacking oligonucleotides for probing single-base mismatches in DNA.
abstract: [reaction: see text] Fluorescence can be quenched through PET from the fluorophore to neighboring C, T, and G bases, but not to the A moiety. The hairpin stem stability arising from pi-stacking and the PET between the pyrene-labeled 2'-deoxynucleotide units and their neighboring bases are the two main factors that affect the operation of these novel fluorescent oligonucleotides.
authors.affiliation: National Research Laboratory, Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Korea.
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Seo
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Ryu
authors.forename: Byeang Hyean
authors.initials: BH
authors.lastname: Kim
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes; D004247:DNA