title: [Cloning and bioinformatic analysis of a novel gene HCR2 up-regulated in aorta of hypercoagulable rat].
abstract: OBJECTIVE
  The aim of this study was to clone the full-length cDNA of HCR2 up-regulated in aorta of hypercoagulable rat and make the relevant bioinformatic analysis.
  
  
  METHODS
  Rapid amplification of cDNA end (RACE) and nested PCR technique was used to amplify the full-length cDNA of HCR2 from an EST (GenBank accession number BQ901227) which was significantly up-regulated in hypercoagulable rat.
  
  
  RESULTS
  The full-length cDNA of HCR2 has been obtained (GenBank accession number AY234417). Semi-quantitative RT-PCR demonstrated that HCR2 is up-regulated in aorta of hypercoagulable rat. Bioinformatic analysis showed that HCR2 gene is located in rat chromosome 4q11. The full-length cDNA of HCR2 is 1275 bp, coding for a 78 amino acids polypeptide with a theoretical molecular weight of 8841.7 and isoelectric point of 8. 59. This protein has no signal peptide and transmembrane sequence. It may be a nuclear protein. It shares no significant homology with any known protein.
  
  
  CONCLUSIONS
  The successful cloning of HCR2 has laid a solid foundation for further study of its function and its possible role in the occurrence and development of hypercoagulability.
authors.affiliation: Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Zhao
authors.forename: Bing-Wen
authors.initials: BW
authors.lastname: Liu
authors.forename: Huai
authors.initials: H
authors.lastname: Bai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D051381:Rats; D019851:Thrombophilia / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D006023:Glycoproteins; C503567:LOC100364673