Isolation of a thermophilic poly-L-lactide degrading bacterium from compost and its enzymatic characterization.
Thermophilic poly-L-lactide-degrading bacteria were isolated from a garbage fermentor. One of the isolates, strain PL21, was identified as Bacillus smithii based on its physiological properties, sugar assimilation pattern, and partial 16S rDNA sequence. The degradation activity of poly-L-lactide exibited by the culture fluid was parallel to the esterase activity, and the purified enzyme was active against various fatty acid esters and poly-L-lactide, at 60 degrees C and pH 5.
Sakai
K
K
Department of Applied Chemistry, Faculty of Engineering, Oita University, Oita 870-1192, Japan. sakai@cc.oita-u.ac.jp
Kawano
H
H
Iwami
A
A
Nakamura
M
M
Moriguchi
M
M