Accumulation of L-cystathionine by an Escherichia coli mutant deficient in cystathionine beta-lyase.
An Escherichia coli mutant deficient in cystathionine beta-lyase was found to accumulate a substance detectable by ninhydrin reaction and chloride platinic acid reaction in its cells (rarely in the culture supernatant) when cultured with a limited amount (50-200 microg/ml) of L-methionine to support the growth. The product was released by freezing treatment and isolated by ion-exchange chromatography (cation exchange resin: Daiaion SK1B). It was identified as L-cystathionine by liquid chromatography-mass spectrometry, 13C- and 1H-nuclear magnetic resonance analyses and high-performance liquid chromatography (as its 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate derivative).
Nishi
Takuya
T
Graduate School of Integrated Science and Art, University of East Asia, 2-1 Ichinomiya-gakuenmachi, Shimonoseki City, Yamaguchi 751-0807, Japan.
Tanaka
Kazuhiko
K
Tanaka
Yoshitake
Y
Araki
Kazumi
K
Furihata
Kazuo
K
Onodera
Masayuki
M
Toda
Kiyoshi
K