title: Molecular cloning and tissue distribution of SF-1-related orphan receptors during sexual maturation in female goldfish.
abstract: The steroidogenic factor (SF)-1 gene is one of a number of orphan nuclear receptors, which is a key transcriptional regulator in vertebrate reproduction. We have isolated the SF-1 homologue cDNA from the goldfish pituitary and designed primers for SF-1 on the basis of the highly conserved regions of various known SF-1 superfamily genes. SF-1 cDNA contained 1,948 nucleotides including an open reading frame predicted to encode a protein of 503 amino acids. The distribution pattern of SF-1 in a variety of tissues during sexual maturation in female goldfish was also examined by RT-PCR. Significant variations in the relative expression of SF-1 were observed in different tissues in immature and mature female goldfish. SF-1 transcript in pituitary was significantly higher than other tissues tested in immature and mature female goldfish. Lower expression of SF-1 was observed in the liver but was not detected in brain and ovary of the immature female goldfish. Presence of SF-1 was the predominant expression in the pituitary and brain of mature female goldfish. Also, in the mature female goldfish, a weak transcript was detected in liver and ovary. Interestingly, RT-PCR analysis revealed that the expression of SF-1 became higher in the brain and weaker in the liver in maturing female goldfish. Thus, SF-1 may be regulated in goldfish brain and/or liver. Thus is also tissue-specific distribution of SF-1 during sexual maturation in female goldfish.
authors.affiliation: Division of Marine Environment & Bioscience, Korea Maritime University, 606-791, Busan, Korea. choic@bada.hhu.ac.kr
authors.forename: Cheol Young
authors.initials: CY
authors.lastname: Choi
authors.forename: Hamid R
authors.initials: HR
authors.lastname: Habibi
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006054:Goldfish / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D008099:Liver / Q000502:physiology; D016366:Open Reading Frames; D009928:Organ Specificity / Q000502:physiology; D010902:Pituitary Gland / Q000502:physiology; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000235:genetics; D012741:Sexual Maturation / Q000502:physiology*; D054339:Steroidogenic Factor 1; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018398:Homeodomain Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D014157:Transcription Factors