title: Differential patterns of constitutive intracellular laccases of the vegetative phase of Pleurotus species.
abstract: The constitutive intracellular laccase activity of ten strains of Pleurotus spp. was determined in vitro and by zymograms, using different substrates. Differences in the in vitro activities were observed between all the strains; however, zymogram patterns were only similar for strains within same species, independently of any of the three substrate (2,6-dimethoxyphenol, p-anisidine or o-tolidine) used. The differences observed in the number and positions of the isoforms in the gel suggest that laccase zymograms can be used to differentiate species of this organism.
authors.affiliation: Laboratory of Biotechnology, Research Centre for Biological Sciences, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
authors.forename: M
authors.initials: M
authors.lastname: Téllez-Téllez
authors.forename: C
authors.initials: C
authors.lastname: Sánchez
authors.forename: O
authors.initials: O
authors.lastname: Loera
authors.forename: G
authors.initials: G
authors.lastname: Díaz-Godínez
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism; D001560:Benzidines / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D042845:Laccase / Q000302:isolation & purification / Q000378:metabolism*; D025282:Mycelium / Q000201:enzymology; D020076:Pleurotus / Q000145:classification / Q000201:enzymology*; D011748:Pyrogallol / Q000031:analogs & derivatives / Q000378:metabolism; D013045:Species Specificity; D013379:Substrate Specificity
chemical_list: D000814:Aniline Compounds; D001560:Benzidines; D011748:Pyrogallol; C010120:pyrogallol 1,3-dimethyl ether; C013813:4-anisidine; C009501:2-tolidine; D042845:Laccase