title: Rapid amplification of a water channel-like gene and its flanking sequences from the Methanothermobacter marburgensis genome using a single primer PCR strategy.
abstract: In this study, we isolated a water channel-like gene and its flanking sequences from an archaeal genome using only a set of PCR. This method should greatly simplify gene cloning procedures and improve the efficiency of cloning.
authors.affiliation: Biotechnology Institute, Akita Prefectural University, Ogata, Akita 010-04, Japan.
authors.forename: X
authors.initials: X
authors.lastname: Ding
authors.forename: Y
authors.initials: Y
authors.lastname: Kitagawa