Temperature and water activity effects on growth and temporal deoxynivalenol production by two Argentinean strains of Fusarium graminearum on irradiated wheat grain.
The objective of this study was to determine the effects of water activity (a(W); 0.900-0.995), temperature (5, 15, 25 and 30 degrees C), time of incubation (7-49 days) and their interactions on mycelial growth and deoxynivalenol (DON) production on irradiated wheat grain by two strains of Fusarium graminearum isolated from wheat ears in Argentina. Optimal a(W) levels for growth were in the range 0.950-0.995 with a temperature optima of 25 degrees C. Maximum growth rates were obtained at the highest a(W) (0.995) and 25 degrees C for both strains. No growth was observed at 5 degrees C regardless of the a(W) levels assayed. Both strains were able to growth at the lowest a(W) assayed (0.900), although the temperature ranges allowing growth at this minimal a(W) was 15-25 degrees C. DON was produced the most rapidly (7 days) when incubated at 25 degrees C and 0.995 a(W). All other conditions required 7-14 days before DON was produced on grain. Maximum amounts of DON for both strains were produced at the highest a(W) treatment (0.995) after 6 weeks at 30 degrees C. The range of DON concentrations varied considerably (5 to 140,000 ng g(-1)) depending on a(W) and temperature interaction treatments. Production of DON occurred over a narrower range of a(W) (0.995-0.95) than that for growth (0.995-0.90). DON was more rapidly produced at 25 degrees C but the maximum amount produced was at 30 degrees C. Two-dimensional profiles of a(W) x temperature were developed from these data to identify areas where conditions indicate a significant risk from DON accumulation.
Ramirez
Maria L
ML
Applied Mycology Group, Cranfield Biotechnology Centre, Cranfield University, Silsoe, Bedford MK45 4DT, UK.
Chulze
Sofia
S
Magan
Naresh
N
D003257:Consumer Product Safety; D005506:Food Contamination / Q000032:analysis*; D005514:Food Irradiation; D005516:Food Microbiology; D005670:Fusarium / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D013696:Temperature; D013997:Time Factors; D014255:Trichothecenes / Q000032:analysis / Q000096:biosynthesis*; D014908:Triticum / Q000382:microbiology*; D014867:Water / Q000378:metabolism
D014255:Trichothecenes; D014867:Water; C007262:deoxynivalenol