FISH analysis of pachytene chromosome and DNA fiber of telomere sequence in rice (Oryza sativa L. indica).
Telomere sequences were analyzed by using pachytene chromosome and extended DNA fiber FISH in rice (Oryza, sativa ssp. indica cv. -Guangluai No.4). Pachytene FISH results showed that most of chromosome ends possess the telomere tandem repeats, but the signals on different chromosomes were not the same in intensity. Fiber FISH results indicated that the longest string of beads was 6.55 microm, while the shortest one was 1.82 microm long,which were equal to 16.44 and 4.56 kb correspondingly based on a stretching factor of 2.51 kb/microm. The average value of signal length was 3.62 +/- 1.32 microm, i.e. 9.09 +/- 3.31 kb. It could be estimated that the longest and the shortest as well as the average value were equal to 2,349,651 and 1298 +/- 473 of copy number respectively.
Li
Zong-Yun
ZY
School of Life Science, Xuzhou Normal University, Xuzhou 221116, China. zongyunli@xznu.edu.cn
Qin
Rui
R
Jin
Wei-Wei
WW
Xiong
Zhi-Yong
ZY
Song
Yun-Chun
YC
D032461:Chromosomes, Plant / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D012275:Oryza / Q000235:genetics*; D049471:Pachytene Stage; D018515:Plant Leaves / Q000235:genetics; D020080:Tandem Repeat Sequences; D016615:Telomere / Q000235:genetics*
D018744:DNA, Plant