Effects of tamoxifen and soluble tumor-associated antigens on ovarian structure in mammary tumor-bearing rats.
Previously, we showed that the 66 and 51 kDa soluble tumor-associated antigens (sTAAs) have distinct suppressive effects on chemically induced mammary cancer in rats, both alone and in combination with the hormone-related anticancer drug tamoxifen. Here, we describe the effects of both sTAA and tamoxifen on the histological structure of ovaries in mammary tumor-bearing 30- to 34-week-old rats. Central ovary sections were pooled, the number of the healthy and degenerated follicles were counted, and the size of the corpora lutea was estimated. In follicular development primordial, primary, preantral and antral stages were recognized. Only healthy follicles with visible nuclei were counted. Follicular degeneration was estimated as the number of atretic follicles with follicular remnants. Treatment with tamoxifen alone or in combination with sTAA significantly increased the number of primordial follicles and atretic follicles in the ovaries, and promoted the formation of small follicular cysts. Total area of the corpora lutea decreased. sTAA participated in this process by increasing apoptosis in degenerated follicles.
Kossoy
George
G
Laboratory of Experimental Medicine, Park Rabin, Rehovot, Israel.
Ben-Hur
Herzl
H
Elhayany
Asher
A
Schneider
David F
DF
Kossoy
Nadja
N
Zusman
Itshak
I
D000818:Animals; D000951:Antigens, Neoplasm; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D017209:Apoptosis; D005260:Female; D015674:Mammary Neoplasms, Animal / Q000503:physiopathology*; D006080:Ovarian Follicle / Q000166:cytology* / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013629:Tamoxifen / Q000494:pharmacology*
D000951:Antigens, Neoplasm; D018931:Antineoplastic Agents, Hormonal; D013629:Tamoxifen