title: Electrotransformation of Clostridium paraputrificum M-21 with some plasmids.
abstract: Clostridium paraputrificum M-21 was transformed with several shuttle plasmids constructed for Clostridium acetobutylicum-Escherichia coli and Clostridium perfringens-E. coli by electroporation. The Clostridium stercorarium xylanase gene xyn10B was successfully expressed in C. paraputrificum M-21 and the expressed protein did not suffer from proteolysis by host protease(s). This system will provide us with a genetic tool for genetic and metabolic engineering of this bacterium.
authors.affiliation: Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan. sakka@bio.mie-u.ac.jp
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
authors.forename: Mamiko
authors.initials: M
authors.lastname: Kawase
authors.forename: Daisuke
authors.initials: D
authors.lastname: Baba
authors.forename: Kenji
authors.initials: K
authors.lastname: Morimoto
authors.forename: Shuichi
authors.initials: S
authors.lastname: Karita
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kimura
authors.forename: Kunio
authors.initials: K
authors.lastname: Ohmiya