Proteomic profiling of cholangiocarcinoma cell line treated with pomiferin from Derris malaccensis.
Pomiferin, a prenylated isoflavonoid from Derris malaccensis with strong anti-fungal and anti-oxidant activities, showed cytotoxic activity towards human cholangiocarcinoma cells (HuCCA-1), with IC(50) of 0.9 microg/mL. Pomiferin caused apoptosis, detectable by DNA fragmentation. Two-dimensional PAGE showed increased expression of 12 proteins, namely glucose-regulated protein 75 (grp 75), calcyclin (S100A6), degraded cytokeratin 19, ATP synthase D, ribosomal protein P0, degraded cytokeratin 18 (two spots pI/MW 6.03/29.9 and pI/MW 4.66/21.5), cofilin, annexin A1, triose phosphate isomerase, peroxiredoxin-1, calgizzarin, and profilin. In contrast, cytokeratins (CK) 7, 18 and 19 were down-regulated, and were shown by 1-DE immunodetection to be degraded.
Svasti
Jisnuson
J
Chulabhorn Research Institute, Bangkok, Thailand. scjsv@mahidol.ac.th
Srisomsap
Chantragan
C
Subhasitanont
Pantipa
P
Keeratichamroen
Siriporn
S
Chokchaichamnankit
Daranee
D
Ngiwsara
Lukana
L
Chimnoi
Nitirat
N
Pisutjaroenpong
Somchai
S
Techasakul
Supanna
S
Chen
Shui-Tein
ST
D017209:Apoptosis / Q000187:drug effects*; D001578:Benzopyrans / Q000494:pharmacology*; D045744:Cell Line, Tumor; D018281:Cholangiocarcinoma / Q000737:chemistry* / Q000473:pathology; D053938:DNA Fragmentation; D029866:Derris; D006801:Humans; D007529:Isoflavones; D009363:Neoplasm Proteins / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods
D001578:Benzopyrans; D007529:Isoflavones; D009363:Neoplasm Proteins; C474837:pomiferin