Involvement of AMP-activated protein kinase in fat depot-specific metabolic changes during starvation.
The mechanisms controlling fat depot-specific metabolism are poorly understood. During starvation of mice, downregulation of lipogenic genes, suppression of fatty acid synthesis, and increases in lipid oxidation were all more pronounced in epididymal than in subcutaneous fat. In epididymal fat, relatively strong upregulation of uncoupling protein 2 and phosphoenolpyruvate carboxykinase genes was found. In mice maintained both at 20 and 30 degrees C, AMP-activated protein kinase was activated in epididymal but did not change in subcutaneous fat. Our results suggest that AMPK may have a role in the different response of various fat depots to starvation.
Sponarova
Jana
J
Department of Adipose Tissue Biology, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic.
Mustard
Kirsty J
KJ
Horakova
Olga
O
Flachs
Pavel
P
Rossmeisl
Martin
M
Brauner
Petr
P
Bardova
Kristina
K
Thomason-Hughes
Michaela
M
Braunerova
Radka
R
Janovska
Petra
P
Hardie
D Grahame
DG
Kopecky
Jan
J
D055372:AMP-Activated Protein Kinases; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D004822:Epididymis / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D007473:Ion Channels; D050356:Lipid Metabolism / Q000235:genetics; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics; D051379:Mice; D024101:Mitochondrial Proteins / Q000235:genetics; D009097:Multienzyme Complexes / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology*; D013217:Starvation / Q000201:enzymology* / Q000235:genetics; D000071246:Uncoupling Protein 2
D005230:Fatty Acids, Nonesterified; D007473:Ion Channels; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D009097:Multienzyme Complexes; C000604390:Ucp2 protein, mouse; D000071246:Uncoupling Protein 2; C069767:phosphoenolpyruvate carboxylase kinase; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases