title: Cancer-derived VEGF plays no role in malignant ascites formation in the mouse.
abstract: OBJECTIVE
  Vascular endothelial growth factor (VEGF) is a potent mediator of peritoneal fluid accumulation following tumor progression. This study investigated the role of VEGF secreted by cancerous cells in the formation of malignant ascites.
  
  
  METHODS
  VEGF expression was eliminated by knockdown in the pancreas cancer cell-line PancO2 using vector-based short-hairpin type RNA interference (RNAi). Malignant ascites formation in the mouse was analyzed by intraperitoneal injection of PancO2 cells expressing VEGF or with expression knockdown.
  
  
  RESULTS
  The VEGF knockdown PancO2 cell was successfully established. Knockdown of VEGF did not affect cancer cell proliferation in vitro or in vivo. The volume of ascites following peritoneal expansion of the tumor in VEGF knockdown cells and control cells did not differ statistically in this in vivo study. Moreover, the VEGF concentration in the ascites did not differ statistically.
  
  
  CONCLUSIONS
  Malignant ascites formation might be mediated by VEGF production in noncancerous tissues, such as stromal compartments. An anti-VEGF strategy against malignant ascites could be applied to various tumors regardless of whether they secrete VEGF.
authors.affiliation: Department of Gastroenterology, Graduate School of Medicine, University of Tokyo Hospital, 7-3-1 Hongo, Tokyo 113-8655, Japan.
authors.forename: Bayasi
authors.initials: B
authors.lastname: Guleng
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tateishi
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kanai
authors.forename: Amarsanaa
authors.initials: A
authors.lastname: Jazag
authors.forename: Miki
authors.initials: M
authors.lastname: Ohta
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Asaoka
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ijichi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Jun
authors.initials: J
authors.lastname: Imamura
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Ikenoue
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Fukushima
authors.forename: Keita
authors.initials: K
authors.lastname: Morikane
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyagishi
authors.forename: Kazunari
authors.initials: K
authors.lastname: Taira
authors.forename: Takao
authors.initials: T
authors.lastname: Kawabe
authors.forename: Masao
authors.initials: M
authors.lastname: Omata
mesh_terms: D000818:Animals; D001201:Ascites / Q000209:etiology*; D001483:Base Sequence; D045744:Cell Line, Tumor; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000150:complications / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*
chemical_list: D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A