Quencher-free, end-stacking oligonucleotides for probing single-base mismatches in DNA.
[reaction: see text] Fluorescence can be quenched through PET from the fluorophore to neighboring C, T, and G bases, but not to the A moiety. The hairpin stem stability arising from pi-stacking and the PET between the pyrene-labeled 2'-deoxynucleotide units and their neighboring bases are the two main factors that affect the operation of these novel fluorescent oligonucleotides.
Seo
Young Jun
YJ
National Research Laboratory, Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Korea.
Ryu
Jin Ho
JH
Kim
Byeang Hyean
BH
D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D011721:Pyrenes; D004247:DNA