Role of lupeol and lupeol linoleate on lipemic-oxidative stress in experimental hypercholesterolemia.
Epidemiological studies have shown that there is a positive correlation between the incidence of coronary heart disease (CHD) and the blood cholesterol level. To study the effect of plant derived triterpene, lupeol and its ester lupeol linoleate, on blood lipid status and oxidant stress in heart and hemolysate, male albino Wistar rats were fed high cholesterol diet (normal rat chow supplemented with 4% cholesterol and 1% cholic acid; HCD) for 30 days. A significant increase (p<0.05) in plasma total cholesterol (4.22 fold) and triglycerides (1.7 fold) was observed in HCD fed rats, along with elevated LDL (3.56 fold) and VLDL (1.99 fold) cholesterol and decreased HDL cholesterol (34.14%). Treatment with lupeol and its derivative normalized the lipid profile. The significant increase (p<0.05) in lipid peroxidation (LPO) was paralleled by significantly diminished (p<0.05) activities of antioxidant enzymes (SOD, CAT and GPx) and decreased (p<0.05) concentration of antioxidant molecules (GSH, Vit C and Vit E) in cardiac tissue and hemolysate of HCD fed rats. The oxidative tissue injury in hypercholesterolemic rats was substantiated by the increase in cardiac marker, serum CPK and the drop in its activity in the heart tissue. Lupeol and lupeol linoleate treatment decreased the LPO levels and increased enzymatic and nonenzymatic antioxidants. CPK activity in the treated group was comparable with that of the control. These observations highlight the beneficial effects of the triterpene, lupeol and its linoleate ester derivative, in ameliorating the lipidemic-oxidative abnormalities in the early stage of hypercholesterolemic atherosclerosis.
Sudhahar
Varatharajan
V
Department of Medical Biochemistry, Dr. ALM. Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600 113, India.
Kumar
Sekar Ashok
SA
Varalakshmi
Palaninathan
P
D000818:Animals; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D004195:Disease Models, Animal; D006321:Heart / Q000187:drug effects; D006937:Hypercholesterolemia / Q000097:blood*; D008055:Lipids / Q000097:blood; D008297:Male; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D053978:Pentacyclic Triterpenes; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood; D014315:Triterpenes / Q000494:pharmacology*; D008041:Linoleic Acids; D000094042:Lupanes
D002784:Cholesterol; D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D008055:Lipids; D053978:Pentacyclic Triterpenes; D014280:Triglycerides; D014315:Triterpenes; C403497:lupeol linoleate; D008041:Linoleic Acids; D000094042:Lupanes; C010480:lupeol