Proteomic dataset of Sca-1+ progenitor cells.
Embryonic stem cells (ES cells) can differentiate into endothelial cells and smooth muscle cells (SMCs), which participate in vascular angiogenesis. In this study, we differentiated mouse ES cells into Sca-1(+) cells, which have the potential to serve as vascular progenitor cells, and mapped their proteome by 2-DE using a pH 3-10 non-linear gradient and 12% SDS-polyacrylamide gels. A subset of 300 protein spots was analysed and mapped, with 241 protein spots being identified by their PMF using MALDI-TOF MS or by partial amino acid sequencing using MS/MS. Our protein map is the first of Sca-1(+) progenitor cells and will facilitate the identification of proteins differentially expressed during stem cell differentiation. The proteome of adult arterial SMCs is described in an accompanying paper (in this issue, DOI 10.1002/pmic.200402045). All data are made accessible on our website http://www.vascular-proteomics.com.
Yin
Xiaoke
X
Department of Cardiac and Vascular Sciences, St. George's, University of London, UK.
Mayr
Manuel
M
Xiao
Qingzhong
Q
Mayr
Ursula
U
Tarelli
Edward
E
Wait
Robin
R
Wang
Wen
W
Xu
Qingbo
Q
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004396:Coloring Agents; D017931:DNA Primers; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry; D051379:Mice; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013234:Stem Cells / Q000737:chemistry* / Q000166:cytology
D004396:Coloring Agents; D017931:DNA Primers; D004798:Enzymes; D011506:Proteins; D020543:Proteome