Dietary artepillin C suppresses the formation of aberrant crypt foci induced by azoxymethane in mouse colon.
Artepillin C, a prenylated phenylpropanoid found specifically in Brazilian propolis, has been shown to be a bioavailable antioxidant. In this study, artepillin C was tested for colon cancer-preventing activity using azoxymethane-challenged ddY mice. Oral doses of 80 and 160 mg/kg body weight of propolis or 10mg/kg of artepillin C (equi-amounts to 160 mg propolis) reduced significantly the frequency of colonic aberrant crypt foci (ACF) by 39.2, 43.7 and 43.4%, respectively. In liver of the mice, glutathione S-transferase and NADPH:quinone reductase activity increased with the doses of propolis or artepillin C, and an antioxidant-responsive element (ARE) was found to be activated for binding DNA. Artepillin C is considered to suppress the formation of colonic ACF through the activation of ARE and induction of phase II enzymes in liver.
Shimizu
Kazuo
K
Department of Life Science, Graduate School of Science and Technology, Kobe University, Rokkodai, Nada-ku, Kobe 657-8501, Japan.
Das
Swadesh K
SK
Baba
Masaki
M
Matsuura
Yukinaga
Y
Kanazawa
Kazuki
K
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001397:Azoxymethane; D002273:Carcinogens; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism; D010666:Phenylpropionates / Q000008:administration & dosage / Q000494:pharmacology*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000517:prevention & control*; D011429:Propolis / Q000008:administration & dosage / Q000494:pharmacology; D020218:Response Elements / Q000187:drug effects; D015854:Up-Regulation
D000970:Antineoplastic Agents; D000975:Antioxidants; D002273:Carcinogens; D010666:Phenylpropionates; C102259:artepillin C; D011429:Propolis; D016660:NAD(P)H Dehydrogenase (Quinone); D005982:Glutathione Transferase; D001397:Azoxymethane