Promoter of Arabidopsis thaliana phosphate transporter gene drives root-specific expression of transgene in rice.
The PHT1 promoter::GUS fusion gene was constructed and introduced into Arabidopsis and rice by Agrobacterium-mediated transformation. Strong beta-glucuronidase (GUS) activity was detected in roots and showed phosphate starvation induction both in Arabidopsis and rice. In contrast, GUS activity in aerial tissues such as those of the leaf and stem was low. In situ GUS staining of root tissue indicated that PHT1 was expressed in root hairs and the outer layer of the main roots, but not in root tips. The PHT1 promoter has a desirable character for biotechnological transgene expression in monocot rice plants.
Koyama
Takayoshi
T
Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan.
Ono
Toshiro
T
Shimizu
Masami
M
Jinbo
Tetsuro
T
Mizuno
Rie
R
Tomita
Keiji
K
Mitsukawa
Norihiro
N
Kawazu
Tetsu
T
Kimura
Tetsuya
T
Ohmiya
Kunio
K
Sakka
Kazuo
K
D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D018014:Gene Transfer Techniques; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D028061:Phosphate Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism
D029681:Arabidopsis Proteins; C494975:PT2 protein, Arabidopsis; D028061:Phosphate Transport Proteins; D011994:Recombinant Proteins