Identification of novel indigenous Bacillus thuringiensis isolates.
Internally Transcribed Spacers (ITS) characterization and distribution of Repetitive Extragenic Palindromic (REP) sequences were studied in the genome of 223 field isolates of Bacillus thuringiensis from Madurai, India. They were characterized by morphological, biochemical and molecular methods. One hundred and twenty four of a total 223 isolates fitted ITS characterization of B. thuringiensis varieties known. Significant genomic variation was observed among seven isolates using REP primers. The ITS PCR product (EMBL accession number AJ639659) exhibited 98% nucleotide sequence homology with B. thuringiensis and placed the origin of indigenous isolate LDC-7 closer to B. thuringiensis on the basis of phylogenetic analysis.
Mahalakshmi
A
A
PG Department of Zoology & Research Centre, Lady Doak College, Madurai 625002, India.
Shenbagarathai
R
R
Sujatha
K
K
D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D017931:DNA Primers / Q000737:chemistry; D021901:DNA, Intergenic; D004275:DNA, Ribosomal / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D005821:Genetic Techniques; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D013696:Temperature
D017931:DNA Primers; D021901:DNA, Intergenic; D004275:DNA, Ribosomal