title: Breeding of brewer's yeast by hybridization between a top-fermenting yeast Saccharomyces cerevisiae and a cryophilic yeast Saccharomyces bayanus.
abstract: To improve the fermentability of a top-fermenting yeast at low-temperature, we performed hybridization trials between four top-fermenting Saccharomyces cerevisiae strains and a cryophilic yeast Saccharomyces bayanus YM84 with good fermentability at low-temperature. The hybrids selected using 5-bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside were checked with pulsed-field gel electrophoresis and their brewing performance at the low-temperature of 10.5 degrees C was observed using small-scale (2 l) fermentation trials.
authors.affiliation: Brewing Research Laboratories, Sapporo Breweries Ltd., 10-Okatohme, Yaizu, Shizuoka 425-0013, Japan. Masahide.Sato@sapporobeer.co.jp
authors.forename: Masahide
authors.initials: M
authors.lastname: Sato
authors.forename: Munekazu
authors.initials: M
authors.lastname: Kishimoto
authors.forename: Junji
authors.initials: J
authors.lastname: Watari
authors.forename: Masachika
authors.initials: M
authors.lastname: Takashio