title: Annexin A3 is a potential angiogenic mediator.
abstract: Angiogenesis is a complex process that is regulated by a variety of angiogenic activators and inhibitors. Disruption of the balanced angiogenesis leads to the progress of diseases such as tumor growth, rheumatoid arthritis, and various blood vessel-related disorders. Even though a number of proteins involved in angiogenesis have been identified so far, more protein factors remain to be identified due to complexity of the process. Here we report that annexin A3 (ANXA3) induces migration and tube formation of human umbilical vein endothelial cells. High level of vascular endothelial growth factor (VEGF), a prominent angiogenic factor, is also detected in conditioned medium obtained from cells transfected with ANXA3 expression plasmid. Reporter assays show that ANXA3 enhances hypoxia-inducible factor-1 (HIF-1) transactivation activity. Taken together, our results suggest that ANXA3 is a novel angiogenic factor that induces VEGF production through the HIF-1 pathway.
authors.affiliation: Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333, Republic of Korea.
authors.forename: Jae Eun
authors.initials: JE
authors.lastname: Park
authors.forename: Do Hee
authors.initials: DH
authors.lastname: Lee
authors.forename: Jung A
authors.initials: JA
authors.lastname: Lee
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
authors.forename: Nam-Soon
authors.initials: NS
authors.lastname: Kim
authors.forename: Byoung Chul
authors.initials: BC
authors.lastname: Park
authors.forename: Sayeon
authors.initials: S
authors.lastname: Cho
mesh_terms: D017318:Annexin A3 / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D051793:Hypoxia-Inducible Factor 1 / Q000235:genetics / Q000378:metabolism; D018919:Neovascularization, Physiologic; D015533:Transcriptional Activation / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D051793:Hypoxia-Inducible Factor 1; D042461:Vascular Endothelial Growth Factor A; D017318:Annexin A3