title: [Association between phospholamban gene mutation and dilated cardiomyopathy in the Chengdu area].
abstract: OBJECTIVE
  To find out whether phospholamban (PLN) is the virulence gene of dilated cardiomyopathy (DCM) in the Chinese of Chengdu.
  
  
  METHODS
  DNA was isolated from 89 hospitalized unrelated patients with DCM and 110 healthy Chinese Hans as controls. The PLN mutations was screened by polymerase chain reaction (PCR)-single strand conformation polymorphisms (SSCP) and nucleotide sequence analysis.
  
  
  RESULTS
  In polyacrylamide gel lectrophoresis, no abnormal conformer was found in the two groups. In DNA sequence, no C --> T missense mutation at nucleotide 25 was identified in the DCM patients and the controls. Meanwhile, T --> G missense mutation at nucleotide 116 was not found in the affected individuals and in the controls.
  
  
  CONCLUSIONS
  No PLN gene mutation was found in patients with DCM in Chengdu. This result indicated that PLN gene mutation may not be a common cause for DCM in the Chinese population in Chengdu.
authors.affiliation: Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xin-Yun
authors.initials: XY
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Rao
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Hang
authors.initials: H
authors.lastname: Chen
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002311:Cardiomyopathy, Dilated / Q000235:genetics*; D002681:China; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D000099211:Phospholamban
chemical_list: D002135:Calcium-Binding Proteins; D000099211:Phospholamban