title: Tangential flow filtration technology applicable to large scale recovery of diphtheria toxin.
abstract: The commercial preparation of purified diphtheria toxoid primarily depends on the effective separation of the toxin from a large culture volume of Corynebacterium diphtheriae vaccine strain. Tangential flow or cross-flow filtration is gaining momentum in the processing of biologicals. This study deals with the separation of toxins using a microporous membrane (0.45-microm pore size) with operational variables like transmembrane pressure (TMP), cross flow rate and flux. Under the best conditions, 98% recovery was achieved. The data and conclusions presented here have been drawn from process development work employing available equipment.
authors.affiliation: Pasteur Institute of India, Coonoor 643103, Tamil Nadu, India. piicnr@md4.vsnl.net.in
authors.forename: Bheeman
authors.initials: B
authors.lastname: Sundaran
authors.forename: Chitrambalam
authors.initials: C
authors.lastname: Palaniappan
authors.forename: Yarlagadda Udaya Bhaskara
authors.initials: YU
authors.lastname: Rao
authors.forename: Ratnam
authors.initials: R
authors.lastname: Boopathy
authors.forename: Lakshman Narasingha Rao
authors.initials: LN
authors.lastname: Bhau