Identification of plasma membrane associated mature beta-hexosaminidase A, active towards GM2 ganglioside, in human fibroblasts.
Mature beta-hexosaminidase A has been found associated to the external leaflet of plasma membrane of cultured fibroblasts. The plasma membrane association of beta-hexosaminidase A has been directly determined by cell surface biotinylation followed by affinity chromatography purification of the biotinylated proteins, and by immunocytochemistry. The immunological and biochemical characterization of biotinylated beta-hexosaminidase A revealed that the plasma membrane associated enzyme is fully processed, suggesting its lysosomal origin.
Mencarelli
Simona
S
Department of Medicina Sperimentale e Scienze Biochimiche, University of Perugia, Italy.
Cavalieri
Cristina
C
Magini
Alessandro
A
Tancini
Brunella
B
Basso
Luisa
L
Lemansky
Peter
P
Hasilik
Andrej
A
Li
Yu-Teh
YT
Chigorno
Vanna
V
Orlacchio
Aldo
A
Emiliani
Carla
C
Sonnino
Sandro
S
D002462:Cell Membrane / Q000201:enzymology*; D005347:Fibroblasts / Q000201:enzymology; D005678:G(M2) Ganglioside / Q000378:metabolism*; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D001619:beta-N-Acetylhexosaminidases / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
D005678:G(M2) Ganglioside; D001619:beta-N-Acetylhexosaminidases