Interleukin-12 induces a Th1-like response to Burkholderia mallei and limited protection in BALB/c mice.
We evaluated the effect of interleukin (IL)-12 on the immune response to Burkholderia mallei in BALB/c mice. Mice were vaccinated with non-viable B. mallei cells with or without IL-12. There was a seven- to nine-fold increase in IgG2a levels, and a significant increase in the proliferative response and interferon (IFN)-gamma production by splenocytes from mice that received B. mallei and IL-12. We saw an increase in survivors in the groups of mice that received B. mallei and IL-12 when challenged, compared to mice that received only B. mallei or IL-12. The results suggest that IL-12 can enhance the Th1-like immune response to B. mallei and mediate limited protection from a lethal challenge.
Amemiya
Kei
K
Bacteriology Division, United States Army Research Institute of Infectious Diseases, Frederick, MD 21702, USA. kei.amemita@det.amedd.army.mil
Meyers
Jennifer L
JL
Trevino
Sylvia R
SR
Chanh
Tran C
TC
Norris
Sarah L
SL
Waag
David M
DM
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D042726:Burkholderia mallei / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005896:Glanders / Q000517:prevention & control*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000008:administration & dosage / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018417:Th1 Cells / Q000276:immunology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D018664:Interleukin-12; D007371:Interferon-gamma