RAPD-PCR analysis of various goose populations.
The aim of this study was to genetically analyse by the RAPD-PCR method four indigenous Polish goose breeds, Kartuska (Ka), Lubelska (Lu), Kielecka (Ki) and Podkarpacka (Pd), in order to determine the band-sharing frequency as well as bands characteristic of the evaluated breeds. The birds were maintained as conservative flocks, accounting for a reserve of genetic resources. A total of 102 scorable bands were obtained, their number ranging from 0 to 8, depending on one of seven primers used and the group of birds analyzed, within a mean of 3.64. For each genetic group specific bands with given primers were obtained, suggesting their potential for use as population-specific markers, especially in ex-situ conservation methods. The results also suggest that keeping endangered geese as separate flocks is relevant for their preservation.
Maciuszonek
Anna
A
Department of Animal Biotechnology, University of Technology and Agriculture, Mazowiecka 28, 85-084 Bydgoszcz, Poland.
Grajewski
Bartosz
B
Bednarczyk
Marek
M
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005777:Geese / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers