title: Q/R site editing controls kainate receptor inhibition by membrane fatty acids.
abstract: RNA editing within the pore loop controls the pharmacology and permeation properties of ion channels formed by neuronal AMPA and kainate receptor subunits. Genomic sequences for the glutamate receptor 2 (GluR2) subunit of AMPA receptors and the GluR5 and GluR6 subunits of kainate receptors all encode a neutral glutamine (Q) residue within the channel pore that can be converted by RNA editing to a positively charged arginine (R). Receptors comprised of unedited subunits are permeable to calcium and display inwardly rectifying current-voltage relationships, because of blocking of outward current by intracellular polyamines. In contrast, receptors that include edited subunits conduct less calcium, resist polyamine block, and have relatively linear current-voltage relationships. We showed previously that cis-unsaturated fatty acids, including arachidonic acid and docosahexanoic acid, exert a potent block of native kainate receptors as well as homomeric recombinant receptors formed by transfection of heterologous cells with cDNA for the GluR6(R) subunit. Here, we show that fatty acid blockade of recombinant homomeric and heteromeric kainate receptors is strongly dependent on editing at the Q/R site. Recombinant channels that include unedited subunits exhibit significantly weaker block than channels made up of fully edited subunits. Inhibition of fully edited channels is equivalent at voltages from -70 to +40 mV and is noncompetitive, consistent with allosteric regulation of channel function.
authors.affiliation: Department of Cell Biology and Physiology, Washington University Medical School, St. Louis, Missouri 63110, USA.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Wilding
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhou
authors.forename: James E
authors.initials: JE
authors.lastname: Huettner
mesh_terms: D000818:Animals; D001120:Arginine / Q000235:genetics; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology* / Q000502:physiology*; D005973:Glutamine / Q000235:genetics; D006801:Humans; D007608:Kainic Acid / Q000494:pharmacology; D051379:Mice; D017393:RNA Editing / Q000235:genetics; D018092:Kainic Acid Receptors / Q000819:agonists / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*
chemical_list: D001120:Arginine; D018691:Excitatory Amino Acid Antagonists; D005227:Fatty Acids; D005973:Glutamine; D007608:Kainic Acid; D018092:Kainic Acid Receptors