title: QCM-D studies of attachment and differential spreading of pre-osteoblastic cells on Ta and Cr surfaces.
abstract: The quartz crystal microbalance with dissipation (QCM-D) technique was employed to characterize initial cell adhesion in terms of attachment and spreading of pre-osteoblastic MC3T3-E1 cells on Ta and Cr surfaces. Evaluation of initial cell adhesion established a correlation between input cell number and the shifts in frequency (f) and dissipation (D). The f-shift was found to be much larger in serum-free medium as compared to a medium including serum; hence, initial cell adhesion was subsequently evaluated in serum-free medium. During the first hour of adhesion, we found a positive correlation between the QCM-D f-shift and the average area of the spread cells, as measured by cryo-scanning electron microscopy (cryo-SEM). Finally, the QCM-D technique was used to study cell adhesion on different metal oxide surfaces. Initial cell adhesion on Ta was found to induce a larger f-shift as compared to Cr, indicating larger spreading of cells on Ta. Cryo-SEM data confirmed that spreading of cells on Cr was on average only two-thirds the spreading on Ta. Our results demonstrate that the QCM-D technique is a versatile technique to quickly distinguish initial cell-surface interactions on different biomaterials.
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), University of Aarhus, DK-8000 Aarhus C, Denmark.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Modin
authors.forename: Anne-Louise
authors.initials: AL
authors.lastname: Stranne
authors.forename: Morten
authors.initials: M
authors.lastname: Foss
authors.forename: Mogens
authors.initials: M
authors.lastname: Duch
authors.forename: Jeannette
authors.initials: J
authors.lastname: Justesen
authors.forename: Jacques
authors.initials: J
authors.lastname: Chevallier
authors.forename: Lars K
authors.initials: LK
authors.lastname: Andersen
authors.forename: Anne G
authors.initials: AG
authors.lastname: Hemmersam
authors.forename: Finn S
authors.initials: FS
authors.lastname: Pedersen
authors.forename: Flemming
authors.initials: F
authors.lastname: Besenbacher
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D015374:Biosensing Techniques; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D002857:Chromium; D020285:Cryoelectron Microscopy; D003460:Crystallization; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D011791:Quartz; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D013635:Tantalum
chemical_list: D001672:Biocompatible Materials; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D002857:Chromium; D011791:Quartz; D013635:Tantalum