Increased sulfur precursors and volatiles production by the leek Allium porrum in response to specialist insect attack.
To defend themselves against herbivory, plants use a variety of direct and indirect strategies involving induced increases in secondary substances. Species of the Allium genus (Alliaceae), such as the leek Allium porrum (L.), produce nonprotein sulfur amino acids derived from cysteine, i.e., alk(en)yl-cysteine sulfoxides that are precursors of volatile thiosulfinates and disulfides. These defend most species including the specialist leek moth, Acrolepiopsis assectella. We determined by measuring the increase in the sulfur precursor propyl-cysteine sulfoxide (PCSO) if production of this precursor is induced in response to moth attack and mechanical wounding. The concentration of PCSO was determined by HPLC in 2- or 6-mo-old leeks after attacks of various intensity either by the specialist leek moth or by a generalist moth, Agrotis ipsilon. Injury-induced release of sulfur volatiles was measured by GC/MS after the attacks. Results showed an increase in the production of sulfur compounds in both the precursor and volatile form, occurring only in association with intensive attacks by leek moths. The increase in sulfur precursors also led to an increase in the release of sulfur volatiles. This induced response may provide an effective defense strategy against the plant's main natural enemy, both directly and indirectly by attracting entomophagous insects.
Dugravot
Sebastien
S
Institut de Recherche sur la Biologie de l 'Insecte, UMR CNRS 6035, Faculté des Sciences, Université F. Rabelais, Avenue Monge, Parc Grandmont, 37200 Tours, France.
Mondy
Nathalie
N
Mandon
Nicole
N
Thibout
Eric
E
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D004220:Disulfides / Q000032:analysis / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D009036:Moths / Q000502:physiology*; D019697:Onions / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry*; D011235:Predatory Behavior; D025341:Principal Component Analysis; D012451:Safrole / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D013441:Sulfinic Acids / Q000032:analysis / Q000378:metabolism; D013455:Sulfur / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors; D014835:Volatilization
D004220:Disulfides; D013441:Sulfinic Acids; C100040:thiosulfinic acids; D013455:Sulfur; D003545:Cysteine; D012451:Safrole; C005746:sulfoxide