Chemical properties and NMR spectroscopic identification of certain fungal siderophores.
Siderophores of six fungi viz. Aspergillus sp. ABp4, Aureobacidium pullulans, Penicillium oxalicum, P. chrysosporium, Mycotypha africana and Syncephalastrum racemosum were examined for their (1) electrophoretic mobilities to determine the acidic, basic or neutral charge; (2) Fe (III) binding nature viz., mono-, di-, or trihydroxamate; (3) amino acid composition; and (4) NMR (nuclear magnetic resonance) spectroscopy to determine their structure. Electrophoretic mobilities of siderophores of 3 fungi (P. oxalicum, P. chrysosporium, and M, africana) exhibited net basic charge, siderophores of 2 fungi (Aspergillus sp. ABp4 and S. racemosum) were acidic and 1 fungus (A. pullullans) was neutral. Electrophoresis of ferrated siderophore at pH 2 and colour of the spots indicated that siderophores of Aspergillus sp. ABp4 and P. oxalicum and A. pullulans were trihydroxamates, whereas siderophore of P. chrysosporium was dihydroxamate. Amino acid composition of siderophores purified by XAD-2 column chromatography, revealed the presence of asparagine, histidine, and proline in Aspergillus sp. ABp4, serine and alanine in P. chrysosporium, and valine in M. africana. The structure of purified siderophores as revealed by NMR spectroscopy identified siderophore of AB - 2670 (A. pullulans) as asperchrome F1, and AB-513 (M. africana) as rhizoferrin. The peak obtained for siderophore AB-5 (Aspergillus sp. ABp4) did not show resemblance to any known siderophore, therefore may be an exception.
Baakza
Arefa
A
Department of Life Sciences, Bhavnagar University, Bhavnagar 364002, India.
Dave
B P
BP
Dube
H C
HC
D001230:Aspergillus / Q000378:metabolism; D002845:Chromatography; D004586:Electrophoresis; D032641:Eurotiales / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006877:Hydroxamic Acids / Q000737:chemistry; D007501:Iron / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009090:Mucorales / Q000378:metabolism; D011485:Protein Binding; D017262:Siderophores / Q000378:metabolism*
D005656:Fungal Proteins; D006877:Hydroxamic Acids; D017262:Siderophores; D007501:Iron