Osteopontin is not required for the development of Th1 responses and viral immunity.
Osteopontin (OPN) has been defined as a key cytokine promoting the release of IL-12 and hence inducing the development of protective cell-mediated immunity to viruses and intracellular pathogens. To further characterize the role of OPN in antiviral immunity, OPN-deficient (OPN-/-) mice were analyzed after infection with influenza virus and vaccinia virus. Surprisingly, we found that viral clearance, lung inflammation, and recruitment of effector T cells to the lung were unaffected in OPN-/- mice after influenza infection. Furthermore, effector status of T cells was normal as demonstrated by normal IFN-gamma production and CTL lytic activity. Moreover, activation and Th1 differentiation of naive TCR transgenic CD4+ T cells by dendritic cells and cognate Ag was normal in the absence of OPN in vitro. Contrary to a previous report, we found that OPN-/- mice mounted a normal immune response to Listeria monocytogenes. In conclusion, OPN is dispensable for antiviral immune responses against influenza virus and vaccinia virus.
Abel
Brian
B
Molecular Biomedicine, Swiss Federal Institute of Technology, and.
Freigang
Stefan
S
Bachmann
Martin F
MF
Boschert
Ursula
U
Kopf
Manfred
M
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002454:Cell Differentiation; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D053762:Interleukin-12 Subunit p40; D008070:Lipopolysaccharides / Q000494:pharmacology; D008088:Listeriosis / Q000276:immunology; D008168:Lung / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009975:Orthomyxoviridae / Q000276:immunology*; D053495:Osteopontin; D021122:Protein Subunits / Q000096:biosynthesis; D012795:Sialoglycoproteins / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology*
D000914:Antibodies, Viral; D053762:Interleukin-12 Subunit p40; D008070:Lipopolysaccharides; D021122:Protein Subunits; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma