Imaging and spatial distribution of beta-amyloid peptide and metal ions in Alzheimer's plaques by laser ablation-inductively coupled plasma-mass spectrometry.
Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) has been developed as a new strategy for detection and imaging of beta-amyloid protein in immunohistochemical sections from the brains of a transgenic mouse model of Alzheimer's disease. The distribution of beta-amyloid deposits in tissue was based on measurement of Eu- and Ni-coupled antibodies. The laser-based methodologies (spot ablation, single line raster, and two-dimensional imaging) were also used to detect and map trace element distributions and thus provide a novel probe for both elemental and protein data. We also report the combination of laser capture microdissection with LA-ICP-MS as an alternative strategy for microanalysis of immunohistochemical sections.
Hutchinson
Robert W
RW
Centre for Analytical Sciences, The University of Sheffield, Sheffield, UK.
Cox
Alan G
AG
McLeod
Cameron W
CW
Marshall
Peter S
PS
Harper
Alex
A
Dawson
Emma L
EL
Howlett
David R
DR
D000544:Alzheimer Disease / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000276:immunology; D000818:Animals; D001921:Brain / Q000473:pathology; D001923:Brain Chemistry; D005063:Europium / Q000032:analysis*; D007554:Isotopes; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D009532:Nickel / Q000032:analysis*; D058225:Plaque, Amyloid / Q000737:chemistry*
D016229:Amyloid beta-Peptides; D007554:Isotopes; D005063:Europium; D009532:Nickel