PscF is a major component of the Pseudomonas aeruginosa type III secretion needle.
Pseudomonas aeruginosa, a Gram-negative opportunistic pathogen, translocates exoenzymes (Exo) directly into the eukaryotic cell cytoplasm. This is accomplished by a type III secretion/translocation machinery. Here, we show that the P. aeruginosa type III secretory needle structure is composed essentially of PscF, a protein required for secretion and P. aeruginosa cytotoxicity. Partially purified needles, detached from the bacterial surface, are 60-80 nm in length and 7 nm in width, resembling needles from Yersinia spp.. YscF of Yersinia enterocolitica was able to functionally complement the pscF deletion, but required 11 P. aeruginosa-specific amino acids at the N-terminus for its function.
Pastor
Alexandrine
A
Laboratoire de Biochimie et Biophysique des Systèmes Intégrés (UMR 5092 CNRS/CEA/UJF), DRDC/BBSI, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 09, France.
Chabert
Jacqueline
J
Louwagie
Mathilde
M
Garin
Jerôme
J
Attree
Ina
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D015008:Yersinia enterocolitica / Q000737:chemistry / Q000235:genetics
D002352:Carrier Proteins; D004269:DNA, Bacterial; D036341:Intercellular Signaling Peptides and Proteins; C506611:PscF protein, Pseudomonas aeruginosa