mRNAs encoding the Aplysia homologues of fasciclin-I and beta-thymosin are expressed only in the second phase of nerve injury and are differentially segregated in axons regenerating in vitro and in vivo.
Studies using Aplysia californica have demonstrated that transcription after nerve injury occurs during a rapid, transient first phase and a delayed, prolonged second phase. Although the second phase is especially important for regeneration, the mRNAs produced during this phase have not been identified. We characterized two such mRNAs following axotomy. One encodes a novel fasciclin-I homologue, Aplysia fasciclin-like protein (apFasP), and the other encodes Aplysia beta-thymosin (apbetaT). In addition to mRNA synthesis, proteins required for regeneration must be available at the site of growth, and the transport and local translation of certain extrasomatic mRNAs aids in this process. We found apbetaT and apFasP proteins and mRNA at growth cones in vitro. However, only the mRNA for apbetaT was present in regenerating axons in vivo. This implies that the membrane protein apFasP is supplied by rapid transport from the soma, whereas the soluble apbetaT is synthesized locally.
Colby
Geoffrey P
GP
Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA.
Sung
Ying-Ju
YJ
Ambron
Richard T
RT
D000818:Animals; D001048:Aplysia; D001369:Axons / Q000378:metabolism*; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002452:Cell Count / Q000379:methods; D003001:Cloning, Molecular; D007839:Functional Laterality; D017952:Ganglia, Invertebrate / Q000473:pathology; D015870:Gene Expression / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D066298:In Vitro Techniques; D008959:Models, Neurological; D009409:Nerve Crush / Q000379:methods; D009416:Nerve Regeneration / Q000502:physiology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D020539:Sequence Analysis, Protein; D017385:Sequence Homology; D013947:Thymosin / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D020196:Trauma, Nervous System / Q000378:metabolism*
D015816:Cell Adhesion Molecules, Neuronal; D012333:RNA, Messenger; C051641:fasciclin I; D013947:Thymosin