Modelling the effect of a heat shock and germinant concentration on spore germination of a wild strain of Bacillus cereus.
The effect of different concentrations of L-alanine on the germination kinetics of a strain of Bacillus cereus isolated from liquid egg after heat shock during sporulation was studied. Germination at 30 degrees C and was followed by spectrophotometry. The higher the concentration of L-alanine within the range 100-1 mM the faster was the germination obtained. On the other hand, the application of a heat shock had an effect on the germination producing a diminution on the germination kinetic rates. The Weibull distribution function, a model that has been used for describing inactivation kinetics , was used for modelling germination experimental data. Results indicated that the Weibull distribution function model produced a good description of experimental data.
Collado
J
J
Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Apartado de Correos 73, 46100 Burjassot, Valencia, Spain.
Fernández
A
A
Rodrigo
M
M
Martínez
A
A
D000409:Alanine / Q000378:metabolism / Q000494:pharmacology*; D001409:Bacillus cereus / Q000254:growth & development / Q000502:physiology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D005516:Food Microbiology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008954:Models, Biological; D013171:Spores, Bacterial / Q000254:growth & development*
D000409:Alanine