title: Electrochemical detection of nonlabeled oligonucleotide DNA using biotin-modified DNA(ss) on a streptavidin-modified gold electrode.
abstract: In the electrochemical detection of nonlabeled DNA, it is important to control the bonding at the interface between the DNA and the electrode. Atomic force microscope (AFM) was taken for the commonly used thiol-modified DNA on a gold surface. It was found that the coverage of the DNA was very low. On the other hand, a streptavidin-modified gold electrode provided a much better alternative where DNA hybridization resulted in large changes in the electrochemical reaction responses. This work demonstrates that streptavidin-modified gold electrodes could be used in the development of a new electrochemical protocol for the detection of nonlabeled DNA.
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Jong Wan
authors.initials: JW
authors.lastname: Park
authors.forename: Hea-Yeon
authors.initials: HY
authors.lastname: Lee
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Ryujiro
authors.initials: R
authors.lastname: Yamasaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanno
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Tanaka
authors.forename: Tomoji
authors.initials: T
authors.lastname: Kawai