Synthesis and properties of 2'-O,4'-C-methyleneoxymethylene bridged nucleic acid.
A novel bridged nucleic acid (BNA) analogue, 2'-O,4'-C-methyleneoxymethylene bridged nucleic acid (2',4'-BNA(COC)), was synthesized and incorporated into oligonucleotides. The 2',4'-BNA(COC) modified oligonucleotides showed high binding affinity with an RNA complement and significant enzymatic stability against snake venom phosphodiesterase.
Hari
Yoshiyuki
Y
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan.
Obika
Satoshi
S
Ohnishi
Ryo
R
Eguchi
Ken
K
Osaki
Tomohisa
T
Ohishi
Hirofumi
H
Imanishi
Takeshi
T
D000818:Animals; D018360:Crystallography, X-Ray; D006571:Heterocyclic Compounds / Q000737:chemistry*; D015394:Molecular Structure; D009691:Nucleic Acid Denaturation; D009696:Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D043264:Phosphodiesterase I / Q000378:metabolism; D012911:Snakes; D013696:Temperature
D006571:Heterocyclic Compounds; D009696:Nucleic Acids; D043264:Phosphodiesterase I; C470790:phosphodiesterase I, snake venom