[Spontaneous and induced programmed cell death in suspension cell cultures of cotton (Gossypium hirsutum L.)].
Cotton suspension cells grew well in the MS medium supplemented with 0.1 mg/L 2,4 D and 0.1 mg/L KT. Senescence occurred when the cells were unsubcultured. The cells began to lose their viabilities on the 17th day, and on the 21th day oligonucleosomal sized DNA fragments ( DNA ladder) could be detected. Oligonucleosomal sized DNA fragments ( DNA ladder) was the hallmark of the programmed cell death. Programmed cell death of cotton suspension cells could be induced respectively by some stress factors which included heatshock (42+/-3 degrees C for 8 hours), 10 micromol/L camptothecin, 20 micromol/L fumonisin B1 and 50 mmol/L cycloheximide. The cotton suspension cells which grew in the MS medium supplemented with 0.1 mg/L 2,4 D and 0.1 mg/L KT differred physiologically from the cells in the MS medium supplemented with 0.1 mg/L IBA and 0.1 mg/L KT, and they responded differentially to the heatshock, 10 micromol/L camptothecin and 20 micromol/L fumonisin B1, while the same to 50 mmol/L cycloheximide.
Xia
Qi Zhong
QZ
National Key Laborotory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
Zhang
Xian Long
XL
Nie
Yi Chun
YC
Guo
Xiao Ping
XP
Zhu
Long Fu
LF
D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects; D018744:DNA, Plant / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D037341:Fumonisins / Q000494:pharmacology; D003368:Gossypium / Q000166:cytology* / Q000187:drug effects / Q000235:genetics; D006358:Hot Temperature
D018744:DNA, Plant; D037341:Fumonisins; C056933:fumonisin B1; D003513:Cycloheximide; D002166:Camptothecin