Hematopoietic stem cell and marrow stromal cell for spinal cord injury in mice.
We compared the effects of hematopoietic stem cell and marrow stromal cell transplantation for spinal cord injury in mice. From green fluorescent protein transgenic mouse bone marrow, lineage-negative, c-kit- and Sca-1-positive cells were sorted as hematopoietic stem cells and plastic-adherent cells were cultured as marrow stromal cells. One week after injury, hematopoietic stem cells or marrow stromal cells were injected into the lesioned site. Functional recovery was assessed and immunohistochemistry was performed. In the hematopoietic stem cell group, a portion of green fluorescent protein-positive cells expressed glial marker. In the marrow stem cell group, a number of green fluorescent protein and fibronectin-double positive cells were observed. No significant difference was observed in the recovery between both groups. Both hematopoietic stem cells and marrow stromal cells have the potential to restore the injured spinal cord and to promote functional recovery.
Koda
Masao
M
Department of Orthopaedic Surgery, Chiba University Graduate School of Medicine, Chiba University Hospital, 1-8-1 Inohana, Chuo-ku, Chiba, Japan. m-koda@nifty.com
Okada
Seiji
S
Nakayama
Toshinori
T
Koshizuka
Shuhei
S
Kamada
Takahito
T
Nishio
Yutaka
Y
Someya
Yukio
Y
Yoshinaga
Katsunori
K
Okawa
Akihiko
A
Moriya
Hideshige
H
Yamazaki
Masashi
M
D025601:Adenomatous Polyposis Coli Protein / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D017493:Leukocyte Common Antigens / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013119:Spinal Cord Injuries / Q000601:surgery*; D013997:Time Factors
D025601:Adenomatous Polyposis Coli Protein; D005353:Fibronectins; D049452:Green Fluorescent Proteins; D017493:Leukocyte Common Antigens