Genetic engineering using fungal flavohemoglobin for constructing Pseudomonas stutzeri strain emitting less nitrous oxide.
Most denitrifiers produce the greenhouse gas nitrous oxide (N2O) due to insufficient anaerobiosis. We constructed a recombinant Pseudomonas stutzeri strain producing Fusarium oxysporum flavohemoglobin (fhb) and found that it emitted less N2O than the wild-type strain under aerobic and anaerobic conditions. The rate of N2 production was higher than in the wild-type strain after the depletion of oxygen in culture, suggesting that fhb enhanced the reduction of N2O to N2. The strain is the first recombinant bacterial denitrifier that reduces N2O production.
Takaya
Naoki
N
Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan. ntakaya@sakura.cc.tsukuba.ac.jp
Shoun
Hirofumi
H