Development of two pentaplex systems with X-chromosomal STR loci and their allele frequencies in a northeast German population.
In this study we present two new pentaplex systems for the coamplification of X-chromosomal short tandem repeats (STRs). X-penta-1 comprises DXS9898, DXS6807, HPRTB, DXS101, and androgen receptor (ARA); X-penta-2 consists of DXS7133, DXS10011, DXS7424, DXS8377, and DXS8378. In addition, allele frequencies for these loci in a northeast German population comprising 100 females and 105 males were shown. The applicability and usefulness of our two PCR pentaplex approaches in paternity deficiency cases is demonstrated by a combined power of discrimination (PD(c)) for both females and males with PD(c)>0.999999.
Poetsch
Micaela
M
Institute of Forensic Medicine, University of Greifswald, Germany. poetsch@uni-greifswald.de
Petersmann
Heiko
H
Repenning
Antje
A
Lignitz
Eberhard
E
D041321:Chromosomes, Human, X; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D005828:Genetics, Population; D005858:Germany; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D005819:Genetic Markers