title: Overexpression of Aspergillus aculeatus cellobiohydrolase I in Aspergillus oryzae.
abstract: To express the cbhI gene, encoding Aspergillus aculeatus cellobiohydrolase I (CBHI), in Aspergillus oryzae, a plasmid was constructed. The strain that displayed the strongest CBHI activity among the transformants produced about 941 mg/l in liquid culture. It was confirmed by a PCR method that the plasmid was integrated at the niaD locus.
authors.affiliation: Graduate School ofAgriculture and Biological Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Kanamasa
authors.forename: Miho
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Goro
authors.initials: G
authors.lastname: Takada
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Sumitani
authors.forename: Motoo
authors.initials: M
authors.lastname: Araii