title: Genetic polymorphism of alcohol dehydrogenase 3 in alcohol liver cirrhosis and in alcohol chronic pancreatitis.
abstract: OBJECTIVE
  To find the ADH3 genotypes in the Polish population likely to be responsible for higher susceptibility to alcohol disease of the liver and chronic alcohol pancreatitis.
  
  
  METHODS
  The ADH3 genotype and ADH3*1 and ADH3*2 alleles frequencies were examined in 198 patients. Genotyping of the ADH3 was performed using PCR-restriction fragment length polymorphism methods on a white cell DNA.
  
  
  RESULTS
  The genotype ADH3*1/ADH3*1 was found to be significantly more frequent in alcohol abusers compared with non-drinkers. The examinations of the group of alcohol abusers showed that the genotype ADH3*2/ADH3*2 occurred statistically significantly less frequently in patients with chronic pancreatitis than in those without alimentary lesions (healthy drinkers). The alleles ADH3*1 and genotype ADH3*1/ADH3*1 were significantly more frequent in men than in women, whereas alleles ADH3*2 and genotype ADH3*2/ADH3*2 were more common in women.
  
  
  CONCLUSIONS
  The genotype ADH3*2/ADH3*2 is likely to be a protective factor for chronic pancreatitis. Variations in ADH3 genotypes may account for some of the differences in prevalence of alcohol dependence between genders in the Polish population.
authors.affiliation: Department of Gastroenterology and 2 Department of Medical Genetics, Medical University of Lublin, ul. Jaczewskiego 8, Lublin, Poland. Lach.Halina@wp.pl
authors.forename: Halina
authors.initials: H
authors.lastname: Cichoz-Lach
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Partycka
authors.forename: Irina
authors.initials: I
authors.lastname: Nesina
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Celinski
authors.forename: Maria
authors.initials: M
authors.lastname: Słomka
authors.forename: Jacek
authors.initials: J
authors.lastname: Wojcierowski
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000483:Alleles; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008104:Liver Cirrhosis, Alcoholic / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D019512:Pancreatitis, Alcoholic / Q000235:genetics*; D050500:Pancreatitis, Chronic / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics
chemical_list: D017931:DNA Primers; C420272:ADH1C protein, human; D000426:Alcohol Dehydrogenase