Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye.
In this paper, the effects of some chemical and physical factors such as temperature, pH values, glycerol, and divalent metal cations on the protease activity of venom from jellyfish, Rhopilema esculentum Kishinouye, were assayed. Protease activity was dependent on temperature and pH values. Zn(2+), Mg(2+), and Mn(2+) in sodium phosphate buffer (0.02M, pH 8.0) could increase protease activity. Mn(2+) had the best effects among the three metal cations and the effect was about 20 times of that of Zn(2+) or Mg(2+) and its maximal protease activity was 2.3x10(5)U/mL. EDTA could increase protease activity. PMSF had hardly affected protease activity. O-Phenanthroline and glycerol played an important part in inhibiting protease activity and their maximal inhibiting rates were 87.5% and 82.1%, respectively.
Li
Cuiping
C
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Yu
Huahua
H
Liu
Song
S
Xing
Ronge
R
Guo
Zhanyong
Z
Li
Pengcheng
P
D000818:Animals; D002413:Cations, Divalent / Q000494:pharmacology; D002681:China; D003064:Cnidarian Venoms / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010447:Peptide Hydrolases / Q000378:metabolism*; D037401:Scyphozoa; D013816:Thermodynamics; D015032:Zinc / Q000494:pharmacology
D002413:Cations, Divalent; D003064:Cnidarian Venoms; D010447:Peptide Hydrolases; D015032:Zinc