Effect of sugar residues in a glycolipid coated onto a dish on ammonia consumption and gluconeogenesis activity of primary rat hepatocytes.
Coating of 3,4,5-tris(dodecyloxy)benzyl-beta-D-galactopyranoside (TDOB-Gal) on a dish for suspension culture increased the specific ammonia consumption rate of primary rat hepatocytes to 2.4 times that in the case of rat hepatocytes cultured in a dish without coating while there was no increase in the specific urea production rate. TDOB-beta-D-glucopyranoside (TDOB-Glc), -alpha-D-mannnoside, -beta-D-mannoside, 2-acetamido-2-deoxy-beta-D-glucopyranoside, and 2-acetamido-2-deoxy-beta-D-galactopyranoside had almost no influence on the above-mentioned specific rates. In the ammonia loading assay, cells on the dish with TDOB-Gal and -Glc coatings produced glucose, suggesting gluconeogenesis.
Takagi
Mutsumi
M
International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan. takagi@icb.osaka-u.ac.jp
Matsuda
Chikayoshi
C
Sato
Reiko
R
Toma
Kazunori
K
Yoshida
Toshiomi
T