title: A new family of NAD(P)H-dependent oxidoreductases distinct from conventional Rossmann-fold proteins.
abstract: A new family of NAD(P)H-dependent oxidoreductases is now recognized as a protein family distinct from conventional Rossmann-fold proteins. Numerous putative proteins belonging to the family have been annotated as malate dehydrogenase (MDH) or lactate dehydrogenase (LDH) according to the previous classification as type-2 malate/L-lactate dehydrogenases. However, recent biochemical and genetic studies have revealed that the protein family consists of a wide variety of enzymes with unique catalytic activities other than MDH or LDH activity. Based on their sequence homologies and plausible functions, the family proteins can be grouped into eight clades. This classification would be useful for reliable functional annotation of the new family of NAD(P)H-dependent oxidoreductases.
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Muramatsu
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Mihara
authors.forename: Masaru
authors.initials: M
authors.lastname: Goto
authors.forename: Ikuko
authors.initials: I
authors.lastname: Miyahara
authors.forename: Ken
authors.initials: K
authors.lastname: Hirotsu
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kurihara
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Esaki
mesh_terms: D000595:Amino Acid Sequence; D001105:Archaea / Q000201:enzymology*; D004789:Enzyme Activation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry* / Q000145:classification; D011487:Protein Conformation; D017510:Protein Folding; D017386:Sequence Homology, Amino Acid
chemical_list: D009247:NADH, NADPH Oxidoreductases