Evaluation of phosphate removal from water by immobilized phosphate-binding protein PstS.
The phosphate (P(i))-binding protein PstS is a member of a family of periplasmic proteins that act as high-affinity receptors for active transport systems in bacteria. PstS protein purified from Pseudomonas aeruginosa was immobilized to N-hydroxysuccinimide-activated Sepharose, packed into a plastic column (5 x 70 mm), and examined for its potential ability to remove P(i) from water. The PstS-Sepharose column completely removed P(i) from 32P-labeled pond water containing about 0.5 microM P(i) (0.015 mg P per liter). More than 90% of 32P-P(i) that was retained in the column could be eluted by washing with low-pH water.
Kuroda
A
A
Department of Molecular Biotechnology, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8527, Japan.
Kunimoto
H
H
Morohoshi
T
T
Ikeda
T
T
Kato
J
J
Takiguchi
N
N
Miya
A
A
Ohtake
H
H