title: Treatment of Trypanosoma cruzi-infected mice with propolis promotes changes in the immune response.
abstract: Ethanol extract of Bulgarian propolis (Et-Blg) was administered by oral route in doses ranging from 25 to 100mg/kg body weight in experimental Trypanosoma cruzi-infected Swiss mice. Treatment with 50mg Et-Blg/kg body weight/day led to a decrease in parasitemia and showed no hepatic or renal toxic effect. Treatment with Et-Blg led to a decrease in the spleen mass and modulated the initial inflammatory reaction as demonstrated by analysis of the leukocyte profile in peripheral blood, quantification of T cells subsets, and phenotypic markers in the spleen. Preferential expansion of CD8(+) and partial inhibition in the increase of CD4(+)CD69(+) and CD8(+)CD69(+) in CD4(+)CD44(+) and CD8(+)CD44(+) and in the decrease of CD8(+)CD62L in Trypanosoma cruzi-infected mice were also observed. Taken together, our data indicate that treatment of Trypanosoma cruzi-infected mice with Et-Blg interferes with the basic properties of immune cells.
authors.affiliation: Laboratório de Biologia Celular, DUBC, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Andreia P
authors.initials: AP
authors.lastname: Dantas
authors.forename: Bianca P
authors.initials: BP
authors.lastname: Olivieri
authors.forename: Fátima H M
authors.initials: FH
authors.lastname: Gomes
authors.forename: Solange L
authors.initials: SL
authors.lastname: De Castro
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use* / Q000633:toxicity; D014355:Chagas Disease / Q000188:drug therapy*; D005434:Flow Cytometry; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D051379:Mice; D011429:Propolis / Q000627:therapeutic use* / Q000633:toxicity; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology / Q000472:pathogenicity
chemical_list: D000890:Anti-Infective Agents; D011429:Propolis