title: Laboratory evaluation of Artemisia annua L. extract and artemisinin activity against Epilachna paenulata and Spodoptera eridania.
abstract: Ethanolic extract of aerial parts of Artemisia annua L. and artemisinin were evaluated as anti-insect products. In a feeding deterrence assay on Epilachna paenulata Germ (Coleoptera: Coccinellidae) larvae, complete feeding rejection was observed at an extract concentration of 1.5 mg/cm2 on pumpkin leaf tissue. The same concentration produced a feeding inhibition of 87% in Spodoptera eridania (Cramer) (Lepidoptera: Noctuidae). In a no-choice assay, both species ate less and gained less weight when fed on leaves treated with the extract. Complete mortality in E. paenulata and 50% mortality in S. eridania were observed with extract at 1.5 mg/cm2. Artemisinin exhibited a moderate antifeedant effect on E. paenulata and S. eridania at 0.03-0.375 mg/cm2. However, a strong effect on survival and body weight was observed when E. paenulata larvae were forced to feed on leaves treated at 0.03 and 0.075 mg/cm2. Artemisia annua ethanolic extract of aerial parts at 1.5 mg/cm2 showed no phytotoxic effect on pumpkin seedlings.
authors.affiliation: Centro de Excelencia en Productos y Procesos de la Provincia de Córdoba-ACC, Argentina.
authors.forename: María E
authors.initials: ME
authors.lastname: Maggi
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Mangeaud
authors.forename: María C
authors.initials: MC
authors.lastname: Carpinella
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Ferrayoli
authors.forename: Graciela R
authors.initials: GR
authors.lastname: Valladares
authors.forename: Sara M
authors.initials: SM
authors.lastname: Palacios
mesh_terms: D000818:Animals; D019306:Artemisia / Q000378:metabolism* / Q000469:parasitology*; D001517:Coleoptera / Q000502:physiology*; D005247:Feeding Behavior; D006790:Host-Parasite Interactions; D007814:Larva / Q000502:physiology; D010936:Plant Extracts / Q000378:metabolism*; D018515:Plant Leaves
chemical_list: D010936:Plant Extracts