title: Rapid identification of vinca alkaloids by direct-injection electrospray ionisation tandem mass spectrometry and confirmation by high-performance liquid chromatography-mass spectrometry.
abstract: A simple and rapid method for the identification of Vinca alkaloids from a crude extract of Catharanthus roseus G. Don (Apocynaceae) by direct-injection electrospray ionisation (ESI) and tandem mass spectrometry (MS/MS) has been developed. The alkaloids vindoline, vindolidine, vincristine and vinblastine were evaluated in a commercial extract of C. roseus using this method. Catharanthine and its isomers 19S-vindolinine and vindolinine were detected in the commercial product by direct injection ESI/MS/MS and confirmed by preparation and by HPLC-ESI/MS. For the characterisation of different fragment fingerprints, ESI/MS/MS is a sensitive, rapid and convenient technique by which to identify some constituents in complex and mixed plant extracts.
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Yuanpo
authors.initials: Y
authors.lastname: Tai
authors.forename: Cuirong
authors.initials: C
authors.lastname: Sun
authors.forename: Yuanjiang
authors.initials: Y
authors.lastname: Pan
mesh_terms: D030008:Catharanthus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D015394:Molecular Structure; D014748:Vinca Alkaloids / Q000032:analysis* / Q000737:chemistry
chemical_list: D014748:Vinca Alkaloids