Stabilization of white blood cells and immunologic markers for extended analysis using flow cytometry.
We evaluated whole blood samples drawn from 25 healthy donors and 20 HIV-infected donors into K3EDTA and Cyto-Chex BCT blood collection tubes for CD4, CD8, and CD3 cell counts (HIV Panel). Samples collected in Cyto-Chex BCT were stored at room temperature and tested by 4-color flow cytometry at 6 h, 3 days, and 7 days after isolation for CD4, CD8, and CD3 absolute cell counts/microl and compared with samples collected in K3EDTA tubes and tested at 6 h. Regardless of donor type, the samples collected in Cyto-Chex BCT and tested on day 7 yielded results that were statistically indistinguishable (with correlation coefficients of 0.96 or greater) from samples collected in K3EDTA tubes and tested at 6 h. We conclude that whole blood samples collected in Cyto-Chex BCT are stabilized for their marker phenotype for at least 7 days after phlebotomy.
Warrino
Dominic E
DE
Streck, 7002 S. 109th Street, Omaha, NE 68128, United States.
DeGennaro
Louis J
LJ
Hanson
Mary
M
Swindells
Susan
S
Pirruccello
Samuel J
SJ
Ryan
Wayne L
WL
D000328:Adult; D015703:Antigens, CD / Q000097:blood; D015415:Biomarkers; D001793:Blood Preservation / Q000379:methods*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D015703:Antigens, CD; D015415:Biomarkers