[The effect of acetylcholine and atropine on proliferation and differentiation and mAchR1 expression of human SK-N-SH cells].
The SK-N-SH human neuroblastoma cells were treated with acetylcholine (Ach) and atropine (Atro), the proliferation activity of cells was detected by CCK-8 method and the cell cycle distribution was determined by flow cytometry. The changes of the mRNA and protein of mAchR1 and c-fos were analysed by fluorescence quantitative PCR, western blot and flow cytometry indirect-immunofluorescence. Results showed 1 mmol/L Ach could significantly stimulate the proliferation of SK-N-SH cells and 1 mmol/L Atro could prevent SK-N-SH cells from S phase growing into G2/M phase; 1 mmol/L Ach and 1 mmol/L Atro had feedback adjustment on the protein level of mAchR1, but had no significant effect on mRNA level of mAchR1. 1 mmol/L Ach increased the both levels of mRNA and protein of Fos obviously, this effect was reversed by Atro. All phenomena clue us cholinergic receptors joining the events of cell proliferation.
Hu
Xin
X
Institute of Cell and Molecular Biology, Wenzhou Medical College, Wenzhou.
Lu
Jian Xin
JX
D000109:Acetylcholine / Q000494:pharmacology*; D001285:Atropine / Q000494:pharmacology*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016133:Polymerase Chain Reaction; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000235:genetics / Q000378:metabolism*
D016760:Proto-Oncogene Proteins c-fos; D011976:Receptors, Muscarinic; D001285:Atropine; D000109:Acetylcholine