Human immunodeficiency virus type 1 Vpr interacts with antiapoptotic mitochondrial protein HAX-1.
Human immunodeficiency virus type 1 viral protein R (Vpr) is required for viral pathogenesis and has been implicated in T-cell apoptosis through its activation of caspase 3 and caspase 9 and perturbation of mitochondrial membrane potential. To understand better Vpr-mitochondria interaction, we report here the identification of antiapoptotic mitochondrial protein HAX-1 as a novel Vpr target. We show that Vpr and HAX-1 physically associate with each other. Overexpression of Vpr in cells dislocates HAX-1 from its normal residence in mitochondria and creates mitochondrion instability and cell death. Conversely, overexpression of HAX-1 suppressed the proapoptotic activity of Vpr.
Yedavalli
Venkat S R K
VS
Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, Maryland 20892-0460, USA.
Shih
Hsiu-Ming
HM
Chiang
Yu-Ping
YP
Lu
Chun-Yi
CY
Chang
Luan-Yin
LY
Chen
Mao-Yuan
MY
Chuang
Che-Yen
CY
Dayton
Andrew I
AI
Jeang
Kuan-Teh
KT
Huang
Li-Min
LM
D048868:Adaptor Proteins, Signal Transducing; D017209:Apoptosis; D015536:Down-Regulation; D016336:Gene Products, vpr / Q000378:metabolism*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000254:growth & development*; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D054325:vpr Gene Products, Human Immunodeficiency Virus
D048868:Adaptor Proteins, Signal Transducing; D016336:Gene Products, vpr; C104759:HAX1 protein, human; D011506:Proteins; D054325:vpr Gene Products, Human Immunodeficiency Virus