title: Screening for metal ligands by liquid chromatography--ligand-exchange--electrospray mass spectrometry.
abstract: Electrospray ionization mass spectrometry is applied for the selective detection of metal ligands after a post-column continuous-flow ligand-exchange reaction. The detection is based on the specific release of a reporter ligand from a metal-reporter ligand complex by a high affinity ligand. Constant infusion and direct-injection experiments are performed to optimize the method. The on-line coupling of a liquid chromatographic separation prior to the continuous flow ligand-exchange reaction enables the screening for high affinity ligands in complex samples. The feasibility of the method is demonstrated by using several ligands with a different affinity for either Cu(II) or Zn(II) ions. The selectivity of the ligand-exchange detection method can be tuned by the choice of the reporter ligand. This is demonstrated by using either 2,2'-bipyridyl or 5-methyl-1,10-phenanthroline as reporter ligands.
authors.affiliation: Faculty of Sciences, Division of Chemistry, Department of Analytical Chemistry and Applied Spectroscopy, Vrije Universiteit Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands. jg.krabbe@few.vu.nl
authors.forename: J G
authors.initials: JG
authors.lastname: Krabbe
authors.forename: H
authors.initials: H
authors.lastname: Lingeman
authors.forename: W M A
authors.initials: WM
authors.lastname: Niessen
authors.forename: H
authors.initials: H
authors.lastname: Irth
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D008024:Ligands; D008670:Metals / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D008024:Ligands; D008670:Metals