Intraocular pressure measurement over amniotic membrane patch in human subjects.
OBJECTIVE
To investigate the accuracy of intraocular pressure (IOP) measurement over amniotic membrane patching on human ocular surface using Tono-Pen tonometry.


METHODS
The IOP of 28 normal eyes in 14 volunteers (mean age, 27.6 years; range, 25-34) was measured by a handy applanation tonometer and the Tono-Pen XL. Then, a single layer of amniotic membrane (approximately 50 microm) was placed onto the corneal surface of the volunteers and the IOP was again measured using the Tono-Pen XL.


RESULTS
The average IOP using handy applanation tonometry and the Tono-Pen was 14.3 +/- 2.1 and 14.3 +/- 2.4 mm Hg, respectively. In the presence of amniotic membrane, the average IOP was not significantly overestimated (14.7 +/- 2.6 mm Hg). The bias between handy applanation tonometry or the Tono-Pen XL without amniotic membrane and the Tono-Pen XL with amniotic membrane was only -0.40 and -0.18 mm Hg, respectively.


CONCLUSIONS
The Tono-Pen XL is accurate and useful for IOP monitoring even in eyes with amniotic membrane patching.
Hashizume
Akihiro
A
Department of Ophthalmology, Faculty of Medicine, University of Tokyo, Japan.
Usui
Tomohiko
T
Yokoo
Seiichi
S
Mimura
Tatsuya
T
Araie
Makoto
M
Yamagami
Satoru
S
Amano
Shiro
S
D000328:Adult; D000650:Amnion; D001684:Biological Dressings; D006801:Humans; D007429:Intraocular Pressure / Q000502:physiology*; D012016:Reference Values; D015203:Reproducibility of Results; D014065:Tonometry, Ocular / Q000295:instrumentation*