Alterations of the intranucleolar DNA localization caused by simulated microgravity.
The functions of the nucleolus responsible for the biosynthesis of ribosomes in altered gravity are still unclear. The location of nucleolar DNA both in control and simulated microgravity with fluorescent and immunogold cytochemistry was investigated. We determined firstly the redistribution of rDNA in the nucleolar components accompanied with the changes in their ultrastructure. According to it, lowering the level of rDNA transcription in simulated microgravity was firstly proposed.
Sobol
M A
MA
Institute of Botany of NASU, Kyiv, Ukraine. margaret_sobol@yahoo.com
Kordyum
E L
EL
Gonzalez-Camacho
F
F
Rodríguez-Vilarino
V
V
Medina
F J
FJ
D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure; D002843:Chromatin; D018744:DNA, Plant / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000378:metabolism*; D018470:Gravity, Altered; D007150:Immunohistochemistry; D031220:Lepidium sativum / Q000378:metabolism* / Q000648:ultrastructure; D018519:Meristem / Q000378:metabolism / Q000648:ultrastructure; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D012399:Rotation; D018474:Weightlessness Simulation
D002843:Chromatin; D018744:DNA, Plant; D004275:DNA, Ribosomal