title: Degradation of pectic substances by two pectate lyases from a human intestinal bacterium, Clostridium butyricum-beijerinckii group.
abstract: Clostridium butyricum-beijerinckii group, an anaerobic human intestinal bacterium, produced exo-pectate lyase, endo-pectate lyase, and pectin methylesterase, but not polygalacturonase. The production of the two pectate lyases was strongly influenced by the composition of the carbon sources added to the medium. The enzymes secreted by the bacterium into the human large intestine cooperatively digested pectic substances, producing mainly 4,5-unsaturated digalacturonic acid with the participation of the pectin methylesterase. The findings suggest that in the human intestine the bacterium contributes to the digestion of ingested pectic substances.
authors.affiliation: Department of Nutritional Science, Okayama Prefectural University, Soja, Okayama 719-1112, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Ishihara
authors.forename: M
authors.initials: M
authors.lastname: Tanabe
authors.forename: K
authors.initials: K
authors.lastname: Matsubara
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuura