Fibroblast growth stimulation by extracts and compounds of Onosma argentatum roots.
The roots of Onosma argentatum are used traditionally in Turkey for wound healing and burns. The n-hexane-dichloromethane extract of the roots, and four shikonin derivatives (deoxyshikonin, acetyl shikonin, 3-hydroxy-isovaleryl shikonin and 5,8-O-dimethyl acetyl shikonin) isolated from the n-hexane-dichloromethane extract were investigated for their ability to stimulate the growth of human amnion fibroblasts. A range of concentrations was studied and the extract found to stimulate the growth of human amnion fibroblasts in vitro at 0.1 microg/mL whilst 5,8-O-dimethyl acetyl shikonin had the same effect at 0.05-5 microg/mL, although cytotoxicity was observed at 50 microg/mL for all samples. The extract and all the other isolated compounds showed cytotoxicity at 10 microg/mL with the extract and 3-hydroxy-isovaleryl shikonin showing cytotoxicity at 5 microg/mL. It is suggested that any wound healing effect of the roots of Onosma argentatum might be partly due to an additive effect of the shikonin derivatives present.
Ozgen
U
U
Atatürk University, Faculty of Pharmacy, Department of Pharmacognosy, 25240 Erzurum, Turkey. uozgen@atauni.edu.tr
Ikbal
M
M
Hacimuftuoglu
A
A
Houghton
P J
PJ
Gocer
F
F
Dogan
H
H
Coskun
M
M
D000650:Amnion / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D028082:Boraginaceae; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D006133:Growth Substances / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots
D006133:Growth Substances; D010936:Plant Extracts