title: [Macromolecular complex of cardiac troponin I: differences of reactivity with DPC and Dade-Behring assays].
abstract: We have recently identified a macromolecular 440-kDa cardiac troponin I (cTnI) complex after successful percutaneous transluminal coronary angioplasty (PTCA) (Clin Chem 2003; 49: 505-7). The aim of the work was to confirm the existence of such a complex by using another cTnI assay (Dimension RXL, Dade-Behring). We have first studied the correlation between the two assays by using heparinized samples [cTnI(Immulite) = 2.00 cTnI(Dimension) - 0.01 (n = 176; r = 0,987)]. Then, cTnI taken 120 minutes after PTCA for two patients was measured with the two assays after fractionation by FPLC. The obtained results confirmed the existence of the 440-kDa cTnI complex and showed that the reactivity between the assays (DPC/Dade-Behring ratio) depended on the nature of the complex: the ratio increased from 0.7 (440-kDa cTnI complex) to 3 (80-kDa cTnI complex) therefore suggesting caution in the comparison between the different cTnI assays in the context of reperfusion therapy.
authors.affiliation: Laboratoire de biochimie médicale, Hôpital Charles Nicolle, Centre hospitalier universitaire et régional de Rouen. alain.lavoinne@chu-rouen.fr
authors.forename: A
authors.initials: A
authors.lastname: Lavoinne
authors.forename: B
authors.initials: B
authors.lastname: Cauliez
authors.forename: A
authors.initials: A
authors.lastname: Duflo-Leroy
authors.forename: C
authors.initials: C
authors.lastname: Tron
authors.forename: H
authors.initials: H
authors.lastname: Eltchaninoff
mesh_terms: D015906:Angioplasty, Balloon, Coronary; D015415:Biomarkers / Q000097:blood; D006801:Humans; D015425:Myocardial Reperfusion; D019210:Troponin I / Q000097:blood*
chemical_list: D015415:Biomarkers; D019210:Troponin I