title: Production of ribonucleotides by autolysis of Hansenula anomala grown on Korean ginseng steaming effluent.
abstract: Hansenula anomala KCCM 11473, which grew well in Korean ginseng steaming effluent, was selected for the production of 5'-ribonucleotides. This strain had high RNA content. Optimal autolysis conditions were established to produce 5'-ribonucleotides (13.9 approximately 28.5 mg/g of biomass) at 55 degrees C and pH 5.0 for 24 h. 5'-Phosphodiesterase and adenyl deaminase were not effective in increasing the yield of 5'-ribonucleotides, but the yield of IMP increased significantly only after the addition of 1.0% adenyl deaminase.
authors.affiliation: Department of Genetic Engineering and Bio-Medicinal Resources Research Center, Paichai University, Taejon 302-735, Korea.
authors.forename: Kim
authors.initials: K
authors.lastname: Jae-Ho
authors.forename: Lee
authors.initials: L
authors.lastname: Byung-Hoon
authors.forename: Lee
authors.initials: L
authors.lastname: Jong-Soo