Selective activation of adrenergic beta1 receptors induces heme oxygenase 1 production in RAW264.7 cells.
We hypothesized that catecholamines through beta-adrenoceptor might modulate macrophage function. We showed that isoproterenol concentration-dependently induced HO-1 production through beta(1)-but not beta(2)-adrenoceptor. Production was increased by forskolin and inhibited by pretreatment with the PKA inhibitor, H-89. Furthermore, induction of HO-1 by isoproterenol effectively protected RAW264.7 cells from effects of glucose oxidase treatment, which was abrogated either by HO-1 inhibitor, ZnPP IX and beta-adenoceptor antagonist, propranolol. Thus, stimulation of HO-1 production through beta(1)-adenoceptors, and via the PKA pathways by isoproterenol, can enable RAW264.7 cells to resist oxidant stress, suggesting that catecholamine hormones may be necessary, at least, to maximize defending role of macrophages.
Sun
Jinji
J
Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.
Kim
Sung Jae
SJ
Park
Min Kyu
MK
Kim
Hye Jung
HJ
Tsoy
Irina
I
Kang
Young Jin
YJ
Lee
Young Soo
YS
Seo
Han Geuk
HG
Lee
Jae Heun
JH
Chang
Ki Churl
KC
D058665:Adrenergic beta-1 Receptor Agonists; D058671:Adrenergic beta-1 Receptor Antagonists; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D015870:Gene Expression / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D007545:Isoproterenol / Q000494:pharmacology; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D011524:Protoporphyrins / Q000494:pharmacology; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics
D058665:Adrenergic beta-1 Receptor Agonists; D058671:Adrenergic beta-1 Receptor Antagonists; D000318:Adrenergic beta-Agonists; D002395:Catecholamines; D011524:Protoporphyrins; D018342:Receptors, Adrenergic, beta-1; C017803:zinc protoporphyrin; D005576:Colforsin; D051547:Heme Oxygenase-1; D017868:Cyclic AMP-Dependent Protein Kinases; D007545:Isoproterenol