title: Development of an automation system for single nucleotide polymorphisms genotyping using bio-strand, a new three-dimensional microarray.
abstract: Previously, we developed a novel three-dimensional microarray system called Bio-Strand, which may be used in various applications including single nucleotide polymorphisms genotyping. In Bio-Strand, samples for detection are immobilized on a one-dimensional thread, which is wound around a cylinder-shaped core to form a three-dimensional thread-and-core structure. The thread-and-core structure is then inserted into a plastic pipette tip, where hybridization and detection are performed. In this study, we have developed an automation system, NIAGALA Bio-Station SDx, which enables automated hybridization and detection during the genotyping procedure using Bio-Strand. Using this system, we have performed the single nucleotide polymorphism (SNP) genotyping of CYP2C, one of the important human cytochrome P450 genes and the results were completely consistent with the genotyping results determined by the TaqMan method.
authors.affiliation: Precision System Science Co., Ltd., 88 Kamihongo, Matsudo, Chiba 270-0025, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Tojo
authors.forename: Junko
authors.initials: J
authors.lastname: Asahina
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Miyashita
authors.forename: Masaaki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Naoko
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Setsuo
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yohda
authors.forename: Hideji
authors.initials: H
authors.lastname: Tajima
mesh_terms: D000465:Algorithms; D001185:Artificial Intelligence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation* / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D010363:Pattern Recognition, Automated / Q000379:methods; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012371:Robotics / Q000379:methods; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D013048:Specimen Handling / Q000295:instrumentation*
chemical_list: C400322:cytochrome P-450 CYP2C subfamily; D003577:Cytochrome P-450 Enzyme System