Vitellogenin in the blood plasma of male cod (Gadus morhua): a sign of oestrogenic endocrine disruption in the open sea?
An ELISA for cod vitellogenin (VTG) has been set up using cod lipovitellin for plate coating and standardisation. The assay has been applied to plasma samples collected from male and female cod caught in three distinct areas around the UK, three areas off the Norwegian coast and also to cod reared initially at an aquaculture site and subsequently maintained at a research station. The aim of the study was to determine whether there were any signs of oestrogenic endocrine disruption in a fish species living offshore. VTG induction was found in male cod caught in the North Sea, the Shetland Box area, in Oslofjord and also in cultivated fish. There was a strong relationship between concentrations of VTG and fish size. There was no evidence that the presence of VTG in the plasma of males is a natural part of their life cycle. On the other hand, the size of fish at which these elevated VTG concentrations appear (ca. 5 kg) is about the size that cod change from feeding primarily on benthic invertebrates to mainly other fish, both benthic and pelagic. The possibility is suggested that large cod pick up oestrogenic endocrine disrupters through the food chain.
Scott
Alexander P
AP
Centre for Environment, Fisheries and Aquaculture Science (Cefas), Weymouth Laboratory, Barrack Road, Weymouth, Dorset, DT4 8UB, UK. A.P.Scott@cefas.co.uk
Katsiadaki
Ioanna
I
Witthames
Peter R
PR
Hylland
Ketil
K
Davies
Ian M
IM
McIntosh
Alistair D
AD
Thain
John
J
D000818:Animals; D049628:Body Size / Q000502:physiology; D002853:Chromatography, Liquid / Q000379:methods; D004032:Diet / Q000662:veterinary; D004527:Egg Proteins; D015387:Egg Proteins, Dietary / Q000097:blood; D052244:Endocrine Disruptors / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D004958:Estradiol / Q000097:blood; D005247:Feeding Behavior; D005260:Female; D020387:Food Chain; D048389:Gadus morhua / Q000097:blood / Q000502:physiology*; D008297:Male; D009792:Oceans and Seas; D012044:Regression Analysis; D013997:Time Factors; D014819:Vitellogenins / Q000097:blood*
D004527:Egg Proteins; D015387:Egg Proteins, Dietary; D052244:Endocrine Disruptors; D014819:Vitellogenins; D004958:Estradiol; C001549:lipovitellin