title: Production of cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene (toluene cis glycol) by Rhodococcus sp. MA 7249.
abstract: An attractive method for producing cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene (toluene cis glycol) was developed employing a cis dihydrodiol dehydrogenase "deficient" strain of Rhodococcus (MA 7249). The toluene cis glycol produced was found to have optical rotations of [alpha]D25 = +25.8 (c 0.45, CH3OH) and +72.8 (c 0.42, CHCl3) which indicated an absolute configuration of (1S,2R) when compared with previously published values. When cultivated in laboratory fermentor in the presence of toluene vapors, MA 7249 reached a toluene cis glycol concentration up to 18 g/l in 110 h. Culture MA 7249 also accumulated cis (1S,2R) dihydrodiols from dihydronaphthalene, biphenyl, chlorobenzene, and styrene.
authors.affiliation: Department of Bioprocess, Merck Research Laboratories, RY 80Y-105, Merck and Co., PO Box 2000, Rayway, NJ 07065, USA.
authors.forename: M
authors.initials: M
authors.lastname: Chartrain
authors.forename: N
authors.initials: N
authors.lastname: Ikemoto
authors.forename: C
authors.initials: C
authors.lastname: Taylor
authors.forename: S
authors.initials: S
authors.lastname: Stahl
authors.forename: V
authors.initials: V
authors.lastname: Sandford
authors.forename: K
authors.initials: K
authors.lastname: Gbewonyo
authors.forename: C
authors.initials: C
authors.lastname: Chirdo
authors.forename: C
authors.initials: C
authors.lastname: Maxwell
authors.forename: J
authors.initials: J
authors.lastname: Osoria
authors.forename: B
authors.initials: B
authors.lastname: Buckland
authors.forename: R
authors.initials: R
authors.lastname: Greasham