Analysis of fatty acid composition in human bone marrow aspirates.
In the present study, the fatty acid composition of bone marrow aspirates and serum phospholipids in nine patients with hematologic diseases was investigated, and the effect of fatty acids on osteoblast differentiation in ST2 cells was examined. The concentrations of oleic acid and palmitic acid were significantly higher in bone marrow aspirates than in serum phospholipids, but the concentrations of other fatty acids did not differ. The rate of alkaline phosphatase positive ST2 cells induced by BMP2 was significantly increased by oleic acid, but was unaffected by the presence or absence of palmitic acid. We conclude that the fatty acid composition of bone marrow aspirates differs from that of serum phospholipids. This difference may affect osteoblast differentiation in the bone marrow microenvironment.
Deshimaru
Ryota
R
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
Ishitani
Ken
K
Makita
Kazuya
K
Horiguchi
Fumi
F
Nozawa
Shiro
S
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001853:Bone Marrow / Q000737:chemistry* / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005227:Fatty Acids / Q000032:analysis* / Q000097:blood; D005260:Female; D006402:Hematologic Diseases / Q000097:blood / Q000378:metabolism / Q000473:pathology; D006801:Humans; D051379:Mice; D008875:Middle Aged; D019301:Oleic Acid / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D019308:Palmitic Acid / Q000494:pharmacology; D010743:Phospholipids / Q000097:blood / Q000737:chemistry
D005227:Fatty Acids; D010743:Phospholipids; D019301:Oleic Acid; D019308:Palmitic Acid