[Detecting LMP1 protein expression and cloning full-length LMP1 gene in extranodal nasal type NK/T-cell lymphoma transplanted tumor models].
OBJECTIVE
To assess the stability and variability of the LMP1 gene and protein in nude-mice transplanted tumor models of extranodal nasal type NK/T-cell lymphoma.


METHODS
PCR and Western Blot were performed to detect the full-length LMP1 gene and protein expression in the established extranodal nasal type NK/ T-cell lymphoma transplanted tumor models and the tissues of donor tumors. Then the sequence of full-length LMP1 gene in transplanted tumor models and donor were compared with the sequence of wild-type B95. 8 and deletion-type CAO cell strains.


RESULTS
LMP1 gene and protein were detected in all the extranodal nasal type NK/T-cell lymphoma transplanted tumor models, and the full-length LMP1 nucleotide sequence homology with the donor tumor was 99. 76%. It was found in sequencing analysis that LMP1 gene sequence possessed both conservative character and specificity, similar to that of CAO cell strains.


CONCLUSIONS
LMP1 gene and protein existed stably in the extranodal nasal type NK/T-cell lymphoma transplanted tumor models, which implied that LMP1 might relate to the maintenance and development of this lymphoma. The nude-mice transplanted tumor models provided an important experimental platform for further studies on the correlation of LMP1 and extranodal nasal type NK/T-cell lymphoma.
Zhao
Sha
S
Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Liu
Wei-Ping
WP
Li
Gan-Di
GD
Zhang
Wen-Yan
WY
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016399:Lymphoma, T-Cell / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D009669:Nose Neoplasms / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014763:Viral Matrix Proteins / Q000032:analysis* / Q000235:genetics*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins