title: Proliferation and stratification of keratinocyte on cultured amniotic epithelial cells for tissue engineering.
abstract: Human amniotic epithelial cells (HAECs) are formed from amnioblasts, separated from the epiblast at about the 8th day after fertilization. Recent studies suggest that HAECs can produce various biologically active substances. In this study, the effects of cultured HAECs on keratinocytes were investigated. First of all, the effect of the medium conditioned by cultured HAECs on the proliferation of keratinocytes was examined. The conditioned medium significantly enhanced the proliferation (P<0.05). Next, the effect of co-culture with HAECs was also examined. The keratinocytes formed a stratified epithelium on day 7 after the start of co-culture. The cultured epithelium formed by the co-culture was five to six layers thick, could be detached by dispase treatment, and had sufficient strength as a sheet. These results suggest that HAECs will be a novel supplemental material for the tissue engineering of skin.
authors.affiliation: Department of Biotechnology, School of Engineering, Nagoya University, Nagoya 464-8603, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Yohei
authors.initials: Y
authors.lastname: Takizawa
authors.forename: Shinkai
authors.initials: S
authors.lastname: Masashige
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Honda
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Hata
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueda
authors.forename: Naohiko
authors.initials: N
authors.lastname: Kuno
authors.forename: Atsuo
authors.initials: A
authors.lastname: Itakura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi