Growth of the hyperthermophilic marine archaeon Aeropyrum pernix in a defined medium.
The nutritional requirements of Aeropyrum pernix were studied to investigate the growth of a hyperthermophilic marine archaeon in a defined medium. Various nutrients including sugars, amino acids, and nucleoside bases were tested to determine their effects on cell growth. It was found that adenine and six amino acids (Arg, Ile, Len, Lys, Met, Val) are essential for the growth of A. pernix. An artificial seawater-based medium, containing 11 amino acids, adenine, vitamins, KH2PO4, Na2S2O3 and trace minerals, enabled the growth of A. pernix at a cell density and specific growth rate similar to those obtained with complex media.
Kim
Kee Woung
KW
Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-Doug, Pohang 790-784, Korea.
Lee
Sun Bok
SB