title: Production of anti-CD2 chimeric antibody by recombinant animal cells.
abstract: Expression vectors for chimeric anti-CD2 antibody were constructed in order to clarify the importance of the expression ratio of heavy (H-) and light (L-) chains of antibody to antibody production in animal cells. The antibody genes were introduced into cells using plasmid DNA vectors or replication-defective retroviral vectors. Productivity was maximal when the expression ratio of H-and L-chains was 1:1, and decreased when the ratio was not equal. We also examined the expression of antibody using one-packed vectors in which the bicistronic expression of H- and L-chain genes was mediated by an internal ribosomal entry site (IRES) sequence derived from encephalomyocarditis virus (EMCV). The translation efficiency was unbalanced between 5'Cap- and IRES-dependent genes. Using the retroviral vectors, it was estimated that the IRES-dependent translation efficiency was 5-fold lower than the 5'Cap-dependent translation efficiency. The cells exhibiting an unbalanced expression of H- and L-chains tended to accumulate H-chain protein.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Akitsu
authors.initials: A
authors.lastname: Hotta
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kamihira
authors.forename: Kanako
authors.initials: K
authors.lastname: Itoh
authors.forename: Mahboob
authors.initials: M
authors.lastname: Morshed
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawabe
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Ono
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Nishijima
authors.forename: Shinji
authors.initials: S
authors.lastname: Iijima