A validated method for the quantification of pimarane and trachylobane diterpenes in the leaves of Croton zambesicus by capillary gas chromatography.
A sensitive and accurate method, combining Soxhlet extraction, solid-phase extraction and capillary gas chromatography, is described for the quantitative determination of four new diterpenes (ent-trachyloban-3beta-ol, ent-18-hydroxy-trachyloban-3-one, ent-trachyloban-3-one and isopimara-7,15-dien-3beta-ol) from the leaves of Croton zambesicus. This is the first method describing the quantification of trachylobane diterpenes in a crude extract. It has been fully validated in order to be able to compare the diterpene composition in other samples of C. zambesicus, which is an important source of trachylobanes.
Block
Sébastien
S
Laboratoire de Pharmacognosie, Unité d'Analyse Chimique et Physico-Chimique des Médicaments, Université Catholique de Louvain, Bruxelles, Belgium.
Brkic
Dejan
D
Hubert
Philippe
P
Quetin-Leclercq
Joëlle
J
D002849:Chromatography, Gas / Q000379:methods*; D029802:Croton / Q000737:chemistry*; D004224:Diterpenes / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004224:Diterpenes; C485923:ent-trachyloban-3-one; C500031:isopimara-7,15-dien-19-oic acid; C464952:trachyloban-3-ol