title: Histologic and molecular characterizations of megakaryocytic leukemia in mice.
abstract: Six cases of megakaryocytic leukemia (MKL) were identified and analyzed for morphology and molecular features. MKL were composed of megakaryocyte lineage cells ranging from immature to quite mature cells. VWF, GATA1 and RUNX1 were strongly expressed in megakaryocytes in both normal spleen and MKL as analyzed by immunohistochemistry (IHC). Altered expression of Meis1, Pbx1 and Psen2 and Lef1 in MKL detected with oligonucleotide microarrays was confirmed by qPCR and IHC. This is the first report of spontaneous MKL in mice, defining VWF as a biomarker for diagnosis and suggesting possible involvement of a series of genes in disease pathogenesis.
authors.affiliation: Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institute of Health, Rockville, MD 20852, USA. xhao@nniaid.nih.gov
authors.forename: Xingpei
authors.initials: X
authors.lastname: Hao
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Shin
authors.forename: Jeff X
authors.initials: JX
authors.lastname: Zhou
authors.forename: Chang Hoon
authors.initials: CH
authors.lastname: Lee
authors.forename: Chen Feng
authors.initials: CF
authors.lastname: Qi
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Naghashfar
authors.forename: Janet W
authors.initials: JW
authors.lastname: Hartley
authors.forename: Torgny N
authors.initials: TN
authors.lastname: Fredrickson
authors.forename: Jerrold M
authors.initials: JM
authors.lastname: Ward
authors.forename: Herbert C
authors.initials: HC
authors.lastname: Morse
mesh_terms: D000818:Animals; D001483:Base Sequence; D019070:Cell Lineage; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics; D017931:DNA Primers; D050982:GATA1 Transcription Factor / Q000235:genetics; D007150:Immunohistochemistry; D039661:Integrin beta3 / Q000235:genetics; D019394:Ki-67 Antigen / Q000235:genetics; D007947:Leukemia, Megakaryoblastic, Acute / Q000235:genetics* / Q000473:pathology*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D014841:von Willebrand Factor / Q000235:genetics
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D017931:DNA Primers; D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D039661:Integrin beta3; D019394:Ki-67 Antigen; C493728:RUNX1 protein, human; D014841:von Willebrand Factor