title: Human coronavirus NL63 is not detected in the respiratory tracts of children with acute Kawasaki disease.
abstract: Kawasaki disease (KD) is a self-limited, systemic vasculitis of children for which an infectious trigger is suspected. Recently, an association between KD and human coronavirus (HCoV)-New Haven (NH) was reported, on the basis of polymerase chain reaction (PCR) with primers that also amplified HCoV-NL63. We investigated the possible association between these HCoVs in the respiratory tract and KD by reverse-transcriptase (RT) PCR and viral culture in a geographically and ethnically diverse population. Only 1 (2%) of 48 patients with acute KD was positive by RT-PCR for HCoV-NL63/NH in a nasopharyngeal swab. These data do not support an association between these HCoVs and KD.
authors.affiliation: Department of Pediatrics, University of California, San Diego, School of Medicine, La Jolla, 92093, USA.
authors.forename: Chisato
authors.initials: C
authors.lastname: Shimizu
authors.forename: Hiroko
authors.initials: H
authors.lastname: Shike
authors.forename: Susan C
authors.initials: SC
authors.lastname: Baker
authors.forename: Francesca
authors.initials: F
authors.lastname: Garcia
authors.forename: Lia
authors.initials: L
authors.lastname: van der Hoek
authors.forename: Taco W
authors.initials: TW
authors.lastname: Kuijpers
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Reed
authors.forename: Anne H
authors.initials: AH
authors.lastname: Rowley
authors.forename: Stanford T
authors.initials: ST
authors.lastname: Shulman
authors.forename: Helen K B
authors.initials: HK
authors.lastname: Talbot
authors.forename: John V
authors.initials: JV
authors.lastname: Williams
authors.forename: Jane C
authors.initials: JC
authors.lastname: Burns
mesh_terms: D002648:Child; D002675:Child, Preschool; D017934:Coronavirus / Q000235:genetics / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000150:complications* / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009080:Mucocutaneous Lymph Node Syndrome / Q000821:virology*; D012137:Respiratory System / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014776:Virus Cultivation