title: Heterologous expression and functional analysis of the gene cluster for the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1.
abstract: Nukacin ISK-1 is a lantibiotic produced by Staphylococcus warneri ISK-1. The gene cluster of nukacin ISK-1 consists of at least nukAMTFEG, ORF1 and ORF7. In this study, we demonstrated the heterologous production of nukacin ISK-1 in Lactococcus lactis by the artificial polycistronic expression of nukAMTFEG-ORF7 under the control of the nisin-controlled expression (NICE) system. Consequently, the recombinant L. lactis showed antimicrobial activity. Mass analysis clarified the presence of nukacin ISK-1 produced in the culture supernatant. These results suggested that the recombinant L. lactis produced nukacin ISK-1 heterologously. Inactivation of nukA, -M or -T resulted in the complete loss of the nukacin ISK-1 production phenotype. This finding suggested that nukAMT are indispensably associated with the biosynthesis of nukacin ISK-1. To our knowledge, this is the first report of the heterologous production of lantibiotic using the NICE system.
authors.affiliation: Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Aso
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Nagao
authors.forename: Hanako
authors.initials: H
authors.lastname: Koga
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Okuda
authors.forename: Youhei
authors.initials: Y
authors.lastname: Kanemasa
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sashihara
authors.forename: Jiro
authors.initials: J
authors.lastname: Nakayama
authors.forename: Kenji
authors.initials: K
authors.lastname: Sonomoto