Finding needles in the haystack: single copy microsatellite loci for Aedes japonicus (Diptera: Culicidae).
First identified in three North American states in 1998, Aedes japonicus japonicus, the Asian bush mosquito, has since spread to 21 states, plus Ontario in Canada, northern France, and Belgium. Analyses of the introduction and expansion of this potentially deadly disease vector will be radically improved by including powerful genetic markers like microsatellites. Useful microsatellite loci have, however, been difficult to identify for mosquitoes in the genus Aedes because of the high amount of repetitive DNA in these species. We isolated single-copy DNA from Ae. j. japonicus and then used a standard enrichment method to identify regions containing microsatellites. Here we describe seven polymorphic microsatellite loci that were tested in American populations of Ae. j. japonicus. These loci were also found to be polymorphic in two other of the four Ae. japonicus subspecies and in Aedes koreicus.
Widdel
Andrea K
AK
Molecular Ecology, PCER, Academy of Natural Sciences, Philadelphia, Pennsylvania 19103, USA. widdel@acnatsci.org
McCuiston
Linda J
LJ
Crans
Wayne J
WJ
Kramer
Laura D
LD
Fonseca
Dina M
DM
D000330:Aedes / Q000145:classification* / Q000235:genetics*; D000818:Animals; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D014644:Genetic Variation; D007303:Insect Vectors / Q000145:classification / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D014481:United States
D004247:DNA