title: A cuticle protein gene from the Japanese oak silkmoth, Antheraea yamamai: gene structure and mRNA expression.
abstract: A cuticle protein gene, AyCP12, from the Japanese oak silkmoth, Antheraea yamamai, was isolated and characterized. The gene spans 1107 bp and consists of one intron and two exons coding for a 112 amino acid polypeptide with a predicted molecular mass of 12,163 Da and a pI of 4.4. The AyCP12 protein contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the AyCP12 cDNA is most homologous to another silkmoth, A. pernyi, cuticle protein ApCP13 (82% protein sequence identity). Northern blot analysis revealed that AyCP12 showed the epidermis-specific expression.
authors.affiliation: Department of Sericulture and Entomology, Miryang National University, 627-130, Miryang, Korea.
authors.forename: Bo Yeon
authors.initials: BY
authors.lastname: Kim
authors.forename: Nam Sook
authors.initials: NS
authors.lastname: Park
authors.forename: Byung Rae
authors.initials: BR
authors.lastname: Jin
authors.forename: Bong Hee
authors.initials: BH
authors.lastname: Lee
authors.forename: Su Il
authors.initials: SI
authors.lastname: Seong
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Jong Su
authors.initials: JS
authors.lastname: Chang
authors.forename: Sang Mong
authors.initials: SM
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry* / Q000302:isolation & purification; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D012333:RNA, Messenger; C047663:cuticle proteins, insects