title: Laccase and Mn-peroxidase production by Coriolus hirsutus strain 075 in a jar fermentor.
abstract: The white-rot fungus Coriolus hirsutus strain 075 excretes considerable amounts of laccase and Mn-peroxidase into culture broth over a brief production time. The effects of agitation speed, temperature, aeration and inoculum amount on laccase production using a 10-l fermentor were studied. The optimum fermentation conditions were a 15% inoculum, an aeration rate of 0.88 vvm, an agitation speed of 160 rpm, and a temperature of 28 degrees C. By optimizing the fermentation conditions, the laccase activity reached 80+/-3 U/ml in 3 d and the purified enzyme output was 30 mg/l. The laccase and Mn-peroxidase were purified by means of isoelectrofocusing and ion-exchange chromatography. The pIs of the laccase isoenzymes were 4.2 and 4.5. Mn-peroxidase had only one isoenzyme with a pI of 3.2. The optimum pH was 4.5 for laccase with syringaldazine as the substrate and 5.0-5.3 for Mn-peroxidase with Mn(+2) and H2O2 as the substrates. The laccase and Mn-peroxidase retained 50% of their activities at 50 degrees C after 55 h and 12 h of incubation time, respectively.
authors.affiliation: A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071 Russia. alena_koroleva@inbox.ru
authors.forename: Olga V
authors.initials: OV
authors.lastname: Koroleva
authors.forename: Elena V
authors.initials: EV
authors.lastname: Stepanova
authors.forename: Valeria P
authors.initials: VP
authors.lastname: Gavrilova
authors.forename: Natalia S
authors.initials: NS
authors.lastname: Yakovleva
authors.forename: Elena O
authors.initials: EO
authors.lastname: Landesman
authors.forename: Ildar S
authors.initials: IS
authors.lastname: Yavmetdinov
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Yaropolov