Effect of Jianweiyuyang granule on gastric ulcer recurrence and expression of VEGF mRNA in the healing process of gastric ulcer in rats.
OBJECTIVE
To investigate the effect of Jianweiyuyang (JWYY) granule on gastric ulcer recurrence and its mechanism in the treatment of gastric ulcer in rats.


METHODS
Gastric ulcer in rats was induced according to Okeba's method with minor modification and the recurrence model was induced by IL-1beta. The expression of vascular endothelial growth factor mRNA (VEGF mRNA) was examined by reverse transcription polymerase chain reaction in gastric ulcer and microvessel density (MVD) adjacent to the ulcer margin was examined by immunohistochemistry.


RESULTS
MVD was higher in the JWYY treatment group (14.0+/-2.62) compared with the normal, model and ranitidine treatment groups (2.2+/-0.84, 8.8+/-0.97, 10.4+/-0.97) in rats (P<0.01). The expression level of VEGF mRNA in gastric tissues during the healing process of JWYY treatment group rats significantly increased compared with other groups (normal group: 0.190+/-0.019, model group: 0.642+/-0.034, ranitidine group: 0.790+/-0.037, P<0.01).


CONCLUSIONS
JWYY granules can stimulate angiogenesis and enhance the expression of VEGF mRNA in gastric ulcer rats. This might be the mechanism for JWYY accelerating the ulcer healing, and preventing the recurrence of gastric ulcer.
Dai
Xing-Ping
XP
Institute of Integrated Traditional Chinese and Western Medicine, Central South University, Changsha 410078, Hunan Province, China.
Li
Jia-Bang
JB
Liu
Zhao-Qian
ZQ
Ding
Xiang
X
Huang
Cheng-Hui
CH
Zhou
Bing
B
D000818:Animals; D001483:Base Sequence; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007375:Interleukin-1 / Q000008:administration & dosage; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000473:pathology; D008517:Phytotherapy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012008:Recurrence; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A