Microfluidic device as a new platform for immunofluorescent detection of viruses.
A bead-based microfluidic device was developed and demonstrated to achieve rapid and sensitive enzyme-linked immunosorbent assay (ELISA) with quantum dots as the labeling fluorophore for virus detection. In comparison to standard ELISA performed on the same virus, the minimal detectable concentration of the target virus was improved from 360 to 22 ng mL-1, the detection time was shortened from >3.25 h to <30 min, and the amount of antibody consumed was reduced by a factor of 14.3.
Liu
Wen-Tso
WT
Division of Environmental Science and Engineering, National University of Singapore, Blk E1A, #07-03, Engineering Drive 2, Singapore 117576. cveliuwt@nus.edu.sg
Zhu
Liang
L
Qin
Qi-Wei
QW
Zhang
Qing
Q
Feng
Hanhua
H
Ang
Simon
S
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation* / Q000379:methods*; D017994:Iridovirus / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D014773:Virology / Q000379:methods