Heat shock protein 70 chaperoned alpha-fetoprotein in human hepatocellular carcinoma cell line BEL-7402.
OBJECTIVE
To investigate the interaction between heat shock protein 70 (HSP70) and ?-fetoprotein (AFP) in human hepatocellular carcinoma (HCC) cell line BEL-7402.


METHODS
The expression and localization of HSP70 and AFP in human HCC cell line BEL-7402 were determined by immunocytochemistry and indirect immunofluorescence cytochemical staining. The interaction between HSP70 and AFP in HCC cells was analyzed by immunoprecipitation and Western blot.


RESULTS
Immunocytochemical staining detection showed that HCC cell BEL-7402 expressed a high level of HSP70 and AFP synchronously. Both were stained in cell plasma. AFP existed in the immunoprecipitate of anti-HSP70 mAb, while there was HSP70 in the immunoprecipitate of anti-AFP mAb.


CONCLUSIONS
HSP70 chaperones AFP in human HCC cell BEL-7402. The interaction between HSP70 and AFP in human HCC cell can be a new route to study the pathogenesis and immunotherapy of HCC.
Wang
Xiao-Ping
XP
Department of Pathology, Capital University of Medical Sciences, Beijing 100054, China. wxpphd@yahoo.com.cn
Wang
Qiao-Xia
QX
Li
Hai-Yan
HY
Chen
Rui-Fen
RF
D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D018840:HSP70 Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000302:isolation & purification / Q000378:metabolism*
D018840:HSP70 Heat-Shock Proteins; D000509:alpha-Fetoproteins