Recombinant protein production in yeasts.
Recombinant DNA (rDNA) technologies (genetic, protein, and metabolic engineering) allow the production of a wide range of peptides, proteins, and biochemicals from naturally nonproducing cells. These technologies, now approx 25 yr old, have become one of the most important technologies developed in the twentieth century. Pharmaceutical products and industrial enzymes were the first biotech products on the world market made by means of rDNA. Despite important advances in rDNA applications in mammalian cells, yeasts still represent attractive hosts for the production of heterologous proteins. In this review we summarize advantages and limitations of the main and most promising yeast hosts.
Porro
Danilo
D
Department of Biotechnology and Bioscience, University of Milano-Bicocca, p.zza della Scienza 2, 20126 Milano, Italy. Danilo.porro@unimib.it
Sauer
Michael
M
Branduardi
Paola
P
Mattanovich
Diethard
D
D005285:Fermentation; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000191:economics / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic; D015003:Yeasts / Q000235:genetics*
D011994:Recombinant Proteins