Trichomonas vaginalis: identification of a triacylglycerol acylhydrolase.
This work describes the identification of a triacylglycerol lipase named TVLip directly onto blood-LB-agar plates by hemolytic screening of a Trichomonas vaginalis cDNA expression library. Sharing significant similarity in the primary sequence with other lipases, the theoretical 3D structure of the TVLip was resolved. The structure reveals the predictive conserved characteristics of other lipases from EC3.1.1.3 group, although presenting one amino acid change in the catalytic triad Ser-His-Asp. Finally, analysis of Northern blot indicates that the expression of the TVLip gene is up-regulated by iron.
Carvalho
Sandra Elisa
SE
Universidade Católica de Brasília, Programa de Pós-graduação em Ciências Genômicas e Biotecnologia, SGAN 916, Brasília DF 70790-160, Brazil.
Freitas
Daniel
D
Murad
André Melro
AM
Franco
Octávio Luiz
OL
Simões-Barbosa
Augusto
A
D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D015971:Gene Expression Regulation, Enzymologic; D006461:Hemolysis / Q000502:physiology; D008049:Lipase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014246:Trichomonas vaginalis / Q000201:enzymology* / Q000235:genetics
D015800:Protozoan Proteins; D008049:Lipase