title: Improved immobilization yields by addition of protecting agents in glutaraldehyde-induced immobilization of protease.
abstract: The effects of peptides and polyethylene glycol (PEG) on the immobilization of protease using glutaraldehyde-activated Lewatit R258-K resin were investigated. When soy peptide was added at 0.1 g/l, the activity recovery and effectiveness factor increased by 20% and 38%, respectively. When PEG4000 (0.5 g/l) was used as a protecting agent, the activity recovery and effectiveness factor were improved by 35% and 60%, respectively, leading to an increased specific activity of the immobilized enzyme. The addition of PEG4000 is suggested to improve the cost-effectiveness for the production of immobilized enzymes.
authors.affiliation: Department of Food Technology, Hoseo University, Asan 336-795, Korea.
authors.forename: H J
authors.initials: HJ
authors.lastname: Chae
authors.forename: E Y
authors.initials: EY
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: In