Differential expression of CD 180 and IgM by B-cell chronic lymphocytic leukaemia cells using mutated and unmutated immunoglobulin VH genes.
We have studied the surface expression of the Toll-like receptor family member CD 180 on cells from 78 patients with B-chronic lymphocytic leukaemia (B-CLL). B-CLL cells had variable levels of CD 180 expression, but this was always less than that expressed by normal blood B cells and was stable for 24 months. Significantly higher levels of CD 180 were expressed by B-CLL cells with mutated IGVH genes compared with those using unmutated IGVH genes. This was in contrast to the higher levels of expression of surface immunoglobulin M by B-CLL cells using unmutated, rather than mutated IGVH genes. CD 180 was functional on B-CLL cells from some of the patients, as shown by the increased expression of CD 86 following incubation in vitro with anti-CD 180. The differential expression of CD 180 amongst B-CLL patients is one more marker that may define more precisely the different biological properties of this heterogeneous disease.
Porakishvili
N
N
Department of Immunology and Molecular Pathology, Royal Free and University College Medical School, London, UK. n.porakishvili@westminster.ac.uk
Kulikova
N
N
Jewell
A P
AP
Youinou
P Y
PY
Yong
K
K
Nathwani
A
A
Heelan
B
B
Duke
V
V
Hamblin
T J
TJ
Wallace
P
P
Ely
P
P
Clark
E A
EA
Lydyard
P M
PM
D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007075:Immunoglobulin M / Q000097:blood*; D016130:Immunophenotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D009154:Mutation
D015703:Antigens, CD; D014408:Biomarkers, Tumor; C092722:CD180 protein, human; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M