title: Bone transport with frozen devitalized bone: an experimental study using rabbits and a clinical application.
abstract: We evaluated the feasibility of bone transport with frozen devitalized bone in the tibia of 20 adult female rabbits. A 1.5-cm segmental defect was created, followed by proximal tibial transverse osteotomy to remove a 2.5-cm segment to be transported after devitalization with liquid nitrogen. The proximal and distal tibia and the devitalized autogenous bone each were fixed with two half-pins. After 2 weeks, transport of the devitalized segment was initiated at 1 mm/day. In a control group, callus formed at proximal and distal osteotomy sites, and distracted callus gradually maturated. The docking site fused and the medullary canal reappeared by 8 weeks after completing distraction. In the frozen-bone group, bone formation proceeded from the proximal tibial end, and the distraction callus slowly matured. The transported segment remained nonviable until revascularization proceeded from its periphery, evident 8 weeks after completion of distraction. Docking sites fused well without infection at pin sites. There was no sign of infection around the pin sites of the devitalized bone. We finally describe similar successful treatment of a 13-year-old girl with tibial deformity resulting from osteofibrous dysplasia who was treated successfully with this procedure. Therefore, bone transport using frozen devitalized bone can regenerate living bone. This experimental model represents the development of a new reconstruction technique of bone transport with devitalized bone.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8641, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sakurakichi
authors.forename: Teruhisa
authors.initials: T
authors.lastname: Yamashiro
authors.forename: Koji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Inoue
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Katsuro
authors.initials: K
authors.lastname: Tomita
mesh_terms: D000293:Adolescent; D000818:Animals; D001848:Bone Diseases, Developmental / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D016025:Bone Transplantation; D002146:Bony Callus / Q000000981:diagnostic imaging / Q000473:pathology; D015925:Cryopreservation; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D018889:Ilizarov Technique; D009584:Nitrogen; D010027:Osteotomy; D011817:Rabbits; D011859:Radiography; D013977:Tibia / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery
chemical_list: D009584:Nitrogen