title: Simultaneous display of bacterial and fungal lipases on the cell surface of Bacillus subtilis.
abstract: A small cell wall-binding domain (CWB(c)) of the Bacillus subtilis peptidoglycan hydrolase CwlC fused to B. subtilis lipase B was able to be localized on the cell wall of B. subtilis. With the aim of developing an efficient lipid-hydrolyzing bacterium with two different lipases on the cell surface, plasmids possessing genes for LipB-CWB(c) and CWB(b)-CutL (the cell wall-binding domain of CwlB fused to the Aspergillus oryzae lipolytic enzyme CutL) were constructed. B. subtilis harboring these plasmids accumulated considerable amounts of both lipases on the cell surface.
authors.affiliation: Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan.
authors.forename: Gota
authors.initials: G
authors.lastname: Kobayashi
authors.forename: Kaori
authors.initials: K
authors.lastname: Fujii
authors.forename: Masakuni
authors.initials: M
authors.lastname: Serizawa
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Junichi
authors.initials: J
authors.lastname: Sekiguchi