Static suspension culture of carrot somatic embryos.
Three culture methods of carrot somatic embryos were compared: conventional shake flask culture, static suspension culture using Erlenmeyer flasks, and static suspension culture using petri dishes. Compared with the former two cultures, the last one resulted in an enhanced ratio of torpedo-shaped embryos and suppressed cotyledonary-stage embryo formation.
Li
Hongmei
H
Department of Biological and Environmental Engineering, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. aa27067@mail.ecc.u-tokyo.ac.jp
Kurata
Kenji
K
D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002478:Cells, Cultured; D018552:Daucus carota / Q000166:cytology / Q000196:embryology* / Q000254:growth & development*; D047108:Embryonic Development / Q000502:physiology*