Modular polyketide synthases: Investigating intermodular communication using 6 deoxyerythronolide B synthase module 2.
A novel variant of 6-deoxyerythronolide B synthase (DEBS) module 2 was constructed to explore the balance between protein-protein-mediated intermodular channeling and intrinsic substrate specificity within DEBS. This construct, termed (N3)Mod2+TE, was co-incubated with a complementary, donor form of the same module, (N5)Mod2(C2), as well as with a mutant of (N5)Mod2(C2) with an inactive ketosynthase domain, in order to determine the extent of intermediate channeling versus substrate diffusion into the downstream module.
Moffet
David A
DA
Department of Chemistry, Box H, Brown University, Providence, RI 02912-9108, USA.
Khosla
Chaitan
C
Cane
David E
DE
D002626:Chemistry, Pharmaceutical / Q000379:methods*; D015195:Drug Design; D004591:Electrophoresis, Polyacrylamide Gel; D007783:Lactones / Q000737:chemistry; D008956:Models, Chemical; D008958:Models, Molecular; D009097:Multienzyme Complexes; D048630:Polyketide Synthases / Q000737:chemistry* / Q000494:pharmacology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity
D007783:Lactones; D009097:Multienzyme Complexes; D048630:Polyketide Synthases