Detecting single stranded DNA with a solid state nanopore.
Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than approximately 11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.
Fologea
Daniel
D
Department of Physics, University of Arkansas, Fayetteville, AR 72701, USA.
Gershow
Marc
M
Ledden
Bradley
B
McNabb
David S
DS
Golovchenko
Jene A
JA
Li
Jiali
J
D004277:DNA, Single-Stranded / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation
D004277:DNA, Single-Stranded