[Polymerization of horseradish peroxidase in the presence of inorganic adsorbents].
Efficiencies of binding between horseradish peroxidase (HRP) and its polymers (HRPp) with inorganic adsorbents (precipitated and coprecipitated) were studied. In aqueous solutions, HRP efficiently adsorbed to aluminum oxide and the coprecipitated sorbent (composed of calcium orthophosphate, magnesium hydroxide, and aluminum hydroxide). HRP readily bound to zinc hydroxide but not to aluminum hydroxide in 25.0 mM bicarbonate buffer (pH 9.0). Several variants of HRP polymerization and HRPp modification with diamines in the presence of Al2O3 and Zn(OH)2 were compared. Synthesis of HRPp according to the scheme comprising HRP activation in solution followed by its polymerization in the presence of Zn(OH)2 appeared the most efficient. HRP and HRPp bound to Zn(OH)2 displayed a high catalytic activity in the presence of high H2O2 concentrations.
Eremin
A N
AN
Makarenko
M V
MV
Budnikova
L P
LP
D000327:Adsorption; D000537:Aluminum Oxide / Q000737:chemistry; D001704:Biopolymers / Q000737:chemistry*; D011232:Chemical Precipitation; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006878:Hydroxides / Q000737:chemistry; D007287:Inorganic Chemicals / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry
D001704:Biopolymers; D006878:Hydroxides; D007287:Inorganic Chemicals; D017967:Zinc Compounds; D006735:Horseradish Peroxidase; D000537:Aluminum Oxide; C052745:zinc hydroxide