Glycine modulates the center response of ON type rod-dominant bipolar cells in carp retina.
Effects of glycine on ON type rod-dominant bipolar cells (RBCs) were studied in isolated, superfused carp retina by intracellular recording technique and in carp retinal slice preparation by whole cell recording. Glycine of 4mM hyperpolarized RBCs and potentiated their light responses to large light spots, which was reversed by co-application of 10 microM strychnine. It was further found that illumination of the receptive field surround did not affect the depolarizing center response of RBCs. The above result therefore suggests that glycine modulates the center response of RBCs. Focal application of glycine to either dendrites or axon terminals of RBCs failed to induce any currents in both isolated cell and retinal slice preparations. On the other hand, glycine of 4mM increased the amplitude of the scotopic electroretinographic PIII component, which reflects the activity of rod photoreceptors. It seems likely that modulation by glycine of the RBC center response may be in part ascribed to a consequence of the potentiation of rod responses by glycine.
Shen
Yin
Y
Institute of Neurobiology, Institute of Brain Science, Fudan University, 220 Handan Road, Shanghai 200433, PR China.
Chen
Ling
L
Ping
Yong
Y
Yang
Xiong-Li
XL
D000818:Animals; D002347:Carps / Q000502:physiology*; D004596:Electroretinography; D005998:Glycine / Q000378:metabolism*; D018684:Glycine Agents / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D009924:Organ Culture Techniques; D010775:Photic Stimulation; D051245:Retinal Bipolar Cells / Q000187:drug effects / Q000502:physiology*; D017948:Retinal Rod Photoreceptor Cells / Q000187:drug effects / Q000502:physiology*; D013331:Strychnine / Q000494:pharmacology
D018684:Glycine Agents; D013331:Strychnine; D005998:Glycine