title: Bacterial communities in petroleum oil in stockpiles.
abstract: Bacterial communities in crude-oil samples from Japanese oil stockpiles were investigated by 16S rRNA gene cloning, followed by denaturing gradient gel electrophoresis (DGGE) analysis. 16S rRNA genes were successfully amplified by PCR after isooctane treatment from three kinds of crude-oil sample collected at four oil stockpiles in Japan. DGGE profiles showed that bacteria related to Ochrobactrum anthropi, Burkholderia cepacia, Stenotrophomonas maltophilia, Propionibacterium acnes, and Brevundimonas diminuta were frequently detected in most crude-oil samples. The bacterial communities differed in the sampling time and layer. Among the predominant bacteria detected in the crude oil, only three species were found for bacteria isolated on agar plates and were related to Burkholderia, Stenotrophomonas, and Propionibacterium, while Ochrobactrum sp. could not be isolated although this species seemed to be the most abundant bacterium in crude oil from the DGGE profiles. Using an archaea-specific primer set, methanogens were found in crude-oil sludge but not in crude-oil samples, indicating that methanogens might be involved in sludge formation in oil stockpiles.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan. yoshidan@bs.naist.jp
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yoshida
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yagi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sato
authors.forename: Noriko
authors.initials: N
authors.lastname: Watanabe
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kuroishi
authors.forename: Kana
authors.initials: K
authors.lastname: Nishimoto
authors.forename: Akira
authors.initials: A
authors.lastname: Yanagida
authors.forename: Tohoru
authors.initials: T
authors.lastname: Katsuragi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kanagawa
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Kurane
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Tani
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D007564:Japan; D010578:Petroleum / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D010578:Petroleum; D012336:RNA, Ribosomal, 16S