Regio- and stereo-selective hydroxylation of abietic acid derivatives by Mucor circinelloides and Mortierella isabellina.
Mucor circinelloides and Mortierella isabellina hydroxylated dehydroabietic acid (DehA). DehA was converted regio- and stereo-selectively by whole cells of Mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mM from 14.7 mM DehA) after 72 h in the cultivation medium containing 3% (v/v) Tween 80. With cells of Ma. isabellina, under the same conditions, 20.5 mM (6.5 g l(-1)) 2-hydroxydehydroabietic acid (alpha/beta=81/19) was formed from 26.4 mM DehA.
Mitsukura
Koichi
K
Department of Biomolecular Science, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
Imoto
Takeshi
T
Nagaoka
Hirokazu
H
Yoshida
Toyokazu
T
Nagasawa
Toru
T
D045784:Abietanes / Q000032:analysis / Q000378:metabolism*; D006900:Hydroxylation; D020094:Mortierella / Q000378:metabolism*; D009089:Mucor / Q000378:metabolism*; D013237:Stereoisomerism
C107978:1-hydroxydehydroabietic acid; D045784:Abietanes; C013913:dehydroabietic acid