Novel fluorogenic substrates for imaging beta-lactamase gene expression.
A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after beta-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of beta-lactamase in cell lysates can be readily detected, and beta-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor.
Gao
Wenzhong
W
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, California 90095-1770, USA.
Xing
Bengang
B
Tsien
Roger Y
RY
Rao
Jianghong
J
D002460:Cell Line; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D015870:Gene Expression; D006801:Humans; D006868:Hydrolysis; D016014:Linear Models; D015394:Molecular Structure; D001618:beta-Lactamases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*
D005456:Fluorescent Dyes; D001618:beta-Lactamases