Enzymatic conversion-based method for screening cyclic dipeptide-producing microbes.
We developed a method for screening cyclic dipeptide-producing microbes by enzymatic conversion. In this method, cyclic dipeptides are detected by the combination of: (i) conversion of cyclic dipeptides to dehydro cyclic dipeptides by cyclo(Leu-Phe) oxidase and (ii) detection of the dehydro derivative by UV spectrophotometry using TLC or HPLC analysis based on the absorbance change caused by the conversion. Using this method, the actinomycete strain A8 was isolated as a cyclic dipeptide-producing strain. The cyclic dipeptides were purified from the microbial extract by enzymatic detection-guided fractionation, and their structures were determined to be cyclo(L-Phe-L-Pro) and cyclo(L-Pro-L-Tyr) by spectroscopic methods.
Arunrattiyakorn
Panarat
P
Graduate School of Natural Science & Technology, Okayama University, Tsushima-naka, Okayama 700-8530, Japan.
Nitoda
Teruhiko
T
Kanzaki
Hiroshi
H
D039903:Actinobacteria / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004151:Dipeptides / Q000096:biosynthesis*; D004798:Enzymes / Q000378:metabolism*; D005658:Fungi / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000096:biosynthesis*
D004151:Dipeptides; D004798:Enzymes; D010456:Peptides, Cyclic