Mineralization of regenerated cellulose hydrogels induced by human bone marrow stromal cells.
The proliferation of cultured human bone marrow stromal cells (HBMSC) on regenerated cellulose hydrogels was assessed. Regenerated cellulose hydrogels showed good rates of HBMSC proliferation, the cells exhibiting a flattened morphology, and after 22 days in culture, the cells had homogeneously colonized the surface of the materials. Moreover, since the early days in culture, between the surface of the materials and attached cells a continuous granulated hydroxyapatite layer was formed. It has been previously demonstrated in vitro, but without cells, that these materials did not mineralize. Hence, it seems that HBMSC promoted the mineralization of the surface.
Granja
P L
PL
INEB-Instituto de Engenharia Biomédica, Rua do Campo Alegre, 823 - 4150-180 Porto, Portugal. pgranja@ineb.up.pt
De Jéso
B
B
Bareille
R
R
Rouais
F
F
Baquey
C
C
Barbosa
M A
MA
D001854:Bone Marrow Cells / Q000166:cytology*; D002113:Calcification, Physiologic; D002130:Calcium Phosphates / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D002482:Cellulose / Q000378:metabolism*; D006801:Humans; D020100:Hydrogels / Q000378:metabolism*; D012038:Regeneration; D017550:Spectroscopy, Fourier Transform Infrared; D017154:Stromal Cells / Q000378:metabolism* / Q000648:ultrastructure
D002130:Calcium Phosphates; D020100:Hydrogels; D002482:Cellulose; C020243:calcium phosphate