Increased oxidative-related mechanisms in the spinal cord injury in old rats.
In the present study, we evaluated the effect of age, in a model of spinal cord injury that was induced by the application of vascular clips to the dura via a four-level T5-T8 laminectomy. Spinal cord injury in old rats resulted in severe trauma characterized by edema and neutrophil infiltration. Immunohistochemical examination demonstrated an increase in immunoreactivity for nitrotyrosine. In contrast, the degree of: (a) spinal cord inflammation and tissue injury (histological score), (b) nitrotyrosine, (c) PARS, and (d) neutrophils infiltration was markedly reduced in spinal cord tissue obtained from young rats. We have also demonstrated that ageing significantly worsened the recovery of limb function and caused an increase in mortality rate when compared with young rats.
Genovese
Tiziana
T
Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, University of Messina, Torre Biologica-Policlinico Universitario Via C. Valeria, Gazzi, Messina 98100, Italy.
Mazzon
Emanuela
E
Di Paola
Rosanna
R
Crisafulli
Concetta
C
Muià
Carmelo
C
Bramanti
Placido
P
Cuzzocrea
Salvatore
S
D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D007150:Immunohistochemistry / Q000379:methods; D007796:Laminectomy / Q000379:methods; D008297:Male; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D051381:Rats; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000401:mortality; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D011064:Poly Adenosine Diphosphate Ribose; C002744:3-nitrotyrosine; D014443:Tyrosine; D009195:Peroxidase