title: The effects of drug delivery via hydrophilic acrylic (hydrogel) intraocular lens systems on the epithelial cells in culture.
abstract: OBJECTIVE
  Secondary posterior subcapsular opacification is still among the most important complications after phacoemulsification.
  
  
  METHODS
  This study was designed to assess the inhibitory effects of drugs delivered via hydrophilic acrylic (hydrogel) intraocular lens (IOL) systems in vitro. Lens epithelial cells were collected from albino rabbits. The following seven groups of hydrogel IOLs were prepared: untreated IOLs and IOLs infiltrated with diclofenac sodium, tranilast, mitomycin C, colchicines, 5-fluorouracil, and ethylenediaminetetraacetic acid. The IOLs were fixed to a Cell Culture Insert; they were then bathed and incubated in minimum essential medium containing cultured lens epithelial cells. Subsequently, a comparative analysis of the cells adhering to the collagen membrane and the lens surfaces was conducted.
  
  
  RESULTS
  Adhesion of lens epithelial cells to the lens surfaces and the collagen membrane was observed in the control group. However, only slight cellular adhesion was found on the surfaces of the IOLs and on the collagen membrane in the treated IOL groups.
  
  
  CONCLUSIONS
  Use of hydrogel IOLs infiltrated with drugs was associated with inhibition of posterior subcapsular opacification in vitro.
authors.affiliation: Department of Ophthalmology, Dokkyo University School of Medicine, Tochigi, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsushima
authors.forename: Kouichiro
authors.initials: K
authors.lastname: Mukai
authors.forename: Norihito
authors.initials: N
authors.lastname: Gotoo
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Yoshida
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yoshida
authors.forename: Muneaki
authors.initials: M
authors.lastname: Sawano
authors.forename: Tadashi
authors.initials: T
authors.lastname: Senoo
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Obara
authors.forename: John I
authors.initials: JI
authors.lastname: Clark
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D002386:Cataract / Q000517:prevention & control; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D003078:Colchicine / Q000008:administration & dosage; D004008:Diclofenac / Q000008:administration & dosage; D016503:Drug Delivery Systems; D004492:Edetic Acid / Q000008:administration & dosage; D004847:Epithelial Cells / Q000473:pathology*; D005472:Fluorouracil / Q000008:administration & dosage; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007908:Lens, Crystalline / Q000473:pathology*; D007910:Lenses, Intraocular; D016685:Mitomycin / Q000008:administration & dosage; D009883:Ophthalmic Solutions / Q000008:administration & dosage; D011183:Postoperative Complications / Q000517:prevention & control; D011817:Rabbits; D062367:ortho-Aminobenzoates / Q000008:administration & dosage
chemical_list: D000180:Acrylic Resins; D009883:Ophthalmic Solutions; D062367:ortho-Aminobenzoates; D004008:Diclofenac; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D016685:Mitomycin; D004492:Edetic Acid; C012293:tranilast; D003078:Colchicine; D005472:Fluorouracil