Suppression of laser-induced choroidal neovascularization by oral administration of SA3443 in mice.
The effect of a synthetic cyclic disulfide compound, SA3443, on neovascularization was investigated. In vitro, enzyme-linked immunosorbent assay and RT-PCR demonstrated that SA3443 suppressed the expression of the hypoxia-induced vascular endothelial growth factor (VEGF) at both protein and mRNA levels in ARPE-19 cells. In vivo, the administration of SA3443 to mice with laser-induced choroidal neovascularization (CNV) suppressed the leakage from the lesions and reduced their size. Furthermore, the expression level of VEGF protein was significantly reduced by the administration of SA3443. Taken together, our results demonstrate that SA3443 suppresses VEGF production and reduces vascular leakage and the growth of mouse experimental CNV.
Muranaka
Kimimasa
K
Department of Ophthalmology, University of Tokyo School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Yanagi
Yasuo
Y
Tamaki
Yasuhiro
Y
Takahashi
Hidenori
H
Usui
Tomohiko
T
Ohashi
Koji
K
Matsuoka
Hidehito
H
Senda
Toshihiko
T
D000284:Administration, Oral; D000693:Anaerobiosis; D000818:Animals; D001392:Azocines / Q000008:administration & dosage / Q000627:therapeutic use*; D020256:Choroidal Neovascularization / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D004220:Disulfides / Q000008:administration & dosage / Q000627:therapeutic use*; D005123:Eye / Q000528:radiation effects; D007834:Lasers; D051379:Mice; D008810:Mice, Inbred C57BL; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D001392:Azocines; D004220:Disulfides; D042461:Vascular Endothelial Growth Factor A; C069788:SA 3443