title: Bioinformatic analysis of circadian gene oscillation in mouse aorta.
abstract: BACKGROUND
  Circadian rhythmicity of many aspects of cardiovascular function-blood pressure, coagulation and contractile function-is well established, as is diurnal variation in important clinical events, such as myocardial infarction and stroke. Here, we undertake studies to globally assess circadian gene expression in murine aorta.
  
  
  RESULTS
  Aortae from mice were harvested at 4-hour intervals for 2 circadian cycles (48 hours). Gene expression was assessed by expression profiling and subjected to a gene ontology bioinformatics analysis. Three hundred thirty transcripts exhibited a circadian pattern of oscillation in mouse aorta, including those intrinsic to the function of the molecular clock. In addition, many genes relevant to protein folding, protein degradation, glucose and lipid metabolism, adipocyte maturation, vascular integrity, and the response to injury are also included in this subset of roughly 7000 genes screened for circadian oscillation.
  
  
  CONCLUSIONS
  Detection of functional cassettes of vascular genes that exhibit circadian regulation in the mouse will facilitate elucidation of the mechanisms by which the molecular clock may interact with environmental variables to modulate cardiovascular function and the response to therapeutic interventions.
authors.affiliation: The Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: R Daniel
authors.initials: RD
authors.lastname: Rudic
authors.forename: Peter
authors.initials: P
authors.lastname: McNamara
authors.forename: Dermot
authors.initials: D
authors.lastname: Reilly
authors.forename: Tilo
authors.initials: T
authors.lastname: Grosser
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Curtis
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Price
authors.forename: Satchidananda
authors.initials: S
authors.lastname: Panda
authors.forename: John B
authors.initials: JB
authors.lastname: Hogenesch
authors.forename: Garret A
authors.initials: GA
authors.lastname: FitzGerald
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000502:physiology*; D002940:Circadian Rhythm / Q000235:genetics*; D019295:Computational Biology; D003624:Darkness; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D009991:Oscillometry; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic