Genes involved in invasion and metastasis of gastric cancer identified by array-based hybridization and serial analysis of gene expression.
Gastric cancer (GC) is still a serious health problem and remains the second most common type of fatal cancer worldwide. Comprehensive gene expression analyses may be useful to identify genes associated with invasion/metastasis in GC. Among them, array-based hybridization and serial analysis of gene expression (SAGE) are currently the most common approaches. Over the past 3 years, several large-scale gene expression studies with array-based hybridization and SAGE have been performed and several genes have been identified. This review describes genes associated with invasion/metastasis in GC which have been identified by array-based hybridization and SAGE. We compared the expression levels of the genes identified by array-based hybridization with our SAGE data. In addition, expression of the candidate genes obtained by SAGE was further investigated by quantitative RT-PCR of 40 GC samples. MIA and GW112 were overexpressed in 10 (25%) and 22 (55%) of 40 GC samples, and the overexpression of these two genes was associated with tumor stage, respectively. We also discuss the significance of HMGB1/amphoterin in invasion and metastasis of GC.
Oue
Naohide
N
Department of Molecular Pathology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
Aung
Phyu Phyu
PP
Mitani
Yoshitsugu
Y
Kuniyasu
Hiroki
H
Nakayama
Hirofumi
H
Yasui
Wataru
W
D001798:Blood Proteins / Q000235:genetics; D016326:Extracellular Matrix Proteins; D015870:Gene Expression; D020869:Gene Expression Profiling; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics; D024243:HMGB1 Protein / Q000502:physiology; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000235:genetics*; D009363:Neoplasm Proteins; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics*; D015854:Up-Regulation
D001798:Blood Proteins; D016326:Extracellular Matrix Proteins; D024243:HMGB1 Protein; C090059:MIA protein, human; D009363:Neoplasm Proteins; C454127:OLFM4 protein, human; D011506:Proteins; D016179:Granulocyte Colony-Stimulating Factor