Proteomic analysis of cadmium-induced protein profile alterations from marine alga Nannochloropsis oculata.
Protein profile alterations following exposure to cadmium were examined in marine alga Nannochloropsis oculata through proteomic analysis. Alterations of the protein expression patterns following 10 muM cadmium treatment were analyzed on 2-dimensional gels. Out of 380 protein spots detected on 2-D gel using Coomassie staining, 11 spots were changed significantly following cadmium treatment. Because of the non-availability of molecular background information on this non-sequenced algal species, cross-species protein identification through ESI-Q-TOF MS/MS was used to identify altered proteins. Two newly induced proteins were identified as malate dehydrogenase orthologue and NADH dehydrogenase orthologue. One suppressed protein was identified to be glyceraldehydes 3-phosphate dehydrogenase A. Protein spot showing a 3-fold increase was identified as mitochondrial NADH: ubiquinone oxidoreductase orthologue. However, we could not find any matches in the database from ESI-Q-TOF MS/MS for the remaining seven proteins, thus only partial peptide sequences of these proteins were found.
Kim
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Division of Life Science, Soonchunhyang University, PO Box 97, 336-600, Asan, Chungnam, Korea.
Yoo
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Lee
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Lee
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MY
D000595:Amino Acid Sequence; D002104:Cadmium / Q000494:pharmacology*; D000460:Chlorophyta / Q000378:metabolism*; D042967:Electron Transport Complex I / Q000096:biosynthesis; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis; D008291:Malate Dehydrogenase / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000096:biosynthesis; D014176:Protein Biosynthesis / Q000187:drug effects*; D040901:Proteomics
D002104:Cadmium; D008291:Malate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D009245:NADH Dehydrogenase; D042967:Electron Transport Complex I