Efficient preparation of optically active ketoprofen by Mucor javanicus lipase immobilized on an inorganic support.
Lipase M from Mucor javanicus, one of nine commercially available hydrolytic enzymes, showed good enantioselectivity (E=50) for racemic ketoprofen trifluoroethyl ester in phosphate buffer (pH 7.0) containing 30% acetone. Lipase M immobilized on Toyonite 200-A showed the best selectivity (E=55) and reactivity. Moreover, the lipase could be recycled at least 5 times.
Kato
K
K
Department of Chemistry, National Industrial Research Institute of Nagoya, Hirate-cho, Kita-ku, Nagoya 462-8510, Japan.
Gong
Y
Y
Saito
T
T
Kimoto
H
H