title: Extracellular secretion of free fatty acids by disruption of a fatty acyl-CoA synthetase gene in Saccharomyces cerevisiae.
abstract: To elucidate the molecular mechanism governing fatty acid transport across the cell membrane, we first isolated a Saccharomyces cerevisiae mutant, B-1, that exhibits a reduced acyl-CoA oxidase activity and an increase in free fatty acid accumulation. Following mutagenesis of B-1, a mutant, YTS51, which secretes free fatty acids, was isolated. The concentration of free fatty acids in the YTS51 culture medium was about 17 times higher than that in B-1. The mutation that causes the fatty acid secretion phenotype occurred at a single allele, and this phenotype was suppressed by the introduction of a single copy of FAA1, a gene for acyl-CoA Synthetase, to the mutant. Although the mutation expressing this phenotype was not within FAA1 in YTS51, the disruption of FAA1 in the wild-type strain resulted in fatty acid secretion even though the level of fatty acid secretion was less than that in YTS51. We consider that YTS51 is a suitable model to elucidate the molecular basis of the fatty acid transport process.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Michinaka
authors.forename: Toshitsugu
authors.initials: T
authors.lastname: Shimauchi
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Aki
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakajima
authors.forename: Seiji
authors.initials: S
authors.lastname: Kawamoto
authors.forename: Seiko
authors.initials: S
authors.lastname: Shigeta
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ono