title: Metalloproteomics: high-throughput structural and functional annotation of proteins in structural genomics.
abstract: A high-throughput method for measuring transition metal content based on quantitation of X-ray fluorescence signals was used to analyze 654 proteins selected as targets by the New York Structural GenomiX Research Consortium. Over 10% showed the presence of transition metal atoms in stoichiometric amounts; these totals as well as the abundance distribution are similar to those of the Protein Data Bank. Bioinformatics analysis of the identified metalloproteins in most cases supported the metalloprotein annotation; identification of the conserved metal binding motif was also shown to be useful in verifying structural models of the proteins. Metalloproteomics provides a rapid structural and functional annotation for these sequences and is shown to be approximately 95% accurate in predicting the presence or absence of stoichiometric metal content. The project's goal is to assay at least 1 member from each Pfam family; approximately 500 Pfam families have been characterized with respect to transition metal content so far.
authors.affiliation: New York Structural GenomiX Research Consortium, Bronx, New York 10461, USA.
authors.forename: Wuxian
authors.initials: W
authors.lastname: Shi
authors.forename: Chenyang
authors.initials: C
authors.lastname: Zhan
authors.forename: Alexander
authors.initials: A
authors.lastname: Ignatov
authors.forename: Babu A
authors.initials: BA
authors.lastname: Manjasetty
authors.forename: Nebojsa
authors.initials: N
authors.lastname: Marinkovic
authors.forename: Michael
authors.initials: M
authors.lastname: Sullivan
authors.forename: Raymond
authors.initials: R
authors.lastname: Huang
authors.forename: Mark R
authors.initials: MR
authors.lastname: Chance
mesh_terms: D001665:Binding Sites; D019295:Computational Biology; D018360:Crystallography, X-Ray; D030562:Databases, Protein; D023281:Genomics; D008667:Metalloproteins / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D011485:Protein Binding; D040901:Proteomics; D020539:Sequence Analysis, Protein; D013052:Spectrometry, X-Ray Emission; D040681:Structural Homology, Protein; D013329:Structure-Activity Relationship
chemical_list: D008667:Metalloproteins