Modulated expression of a nuclear-associated glycoprotein during normal rat liver development and in various hepatoma cells.
Liver plays a major role in systemic detoxification and drug metabolism. NF-164, a protein of 164 kDa predominantly localized in hepatocyte nuclei, was found to be present in increasing amounts during liver maturation. In addition, fetal rat hepatocytes had ten times, and neonatal five times less of this protein than adult hepatocytes. It was also detected in an albumin producing hepatoma cell line, but not in three other lines that have lost several differentiated functions. These data suggest that NF-164 expression is development-dependent and that it may be a marker for both normal and malignant hepatocyte differentiation. NF-164 seems to be liver-specific, since it was not detected in rat brain, spleen, kidney, lung and bovine thymus. It was purified from adult rat hepatocyte nuclei. Its estimated pI is 6.8. Its total amino acid composition and partial amino acid sequence is also being reported. Despite major differences between their respective contents in amino acids, partial sequences showed homologies with carbamyl phosphate synthetase I (CPSI). These observations may suggest that NF-164 also shares some functional features with this enzyme.
Goulet
Francine
F
Laboratory of Tissue Engineering, Hôpital de l'Enfant-Jésus, 1401 18e rue, Québec, QC, Canada. chgfgo@hermes.ulaval.ca
Napa
Ioana Diana
ID
Solomon
Luc
L
Morin
Odette
O
Islam
Nazrul
N
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004586:Electrophoresis / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D022781:Hepatocytes / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism*; D016719:Radioimmunodetection / Q000379:methods; D051381:Rats; D011916:Rats, Inbred F344; D020539:Sequence Analysis, Protein / Q000379:methods; D013347:Subcellular Fractions / Q000378:metabolism
C509973:NF-164 protein, rat; D009687:Nuclear Proteins