title: Overexpression and purification of microbial pro-transglutaminase from Streptomyces cinnamoneum and in vitro processing by Streptomyces albogriseolus proteases.
abstract: Processing and activation of the precursor of an extracellular Streptomyces transglutaminase were achieved by using three Streptomyces proteases (SAM-P20, SAM-P26 and SAM-P45), all of which are widely distributed in Streptomyces. The use of these proteases would allow us to develop a production process for the active form of this enzyme in recombinant bacteria.
authors.affiliation: School of Agriculture, Meiji University, 1-1-1 Higashi-mita, Tama-ku, Kawasaki, Kamagawa 214-8571, Japan. staguchi@isc.meiji.ac.jp
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi
authors.forename: Kumiko
authors.initials: K
authors.lastname: Arakawa
authors.forename: Keiichi
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Shino
authors.initials: S
authors.lastname: Takehana
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takagi
authors.forename: Haruo
authors.initials: H
authors.lastname: Momose