Inhibitory effects of coumarin and acetylene constituents from the roots of Angelica furcijuga on D-galactosamine/lipopolysaccharide-induced liver injury in mice and on nitric oxide production in lipopolysaccharide-activated mouse peritoneal macrophages.
The methanolic extract (200 mg/kg, p.o. and i.p.), principal coumarin constituents (isoepoxypteryxin, anomalin, and praeroside IV), and a polyacetylene constituent (falcarindiol) (25 mg/kg, i.p.) from the roots of Angelica furcijuga protected the liver injury induced by D-galactosamine (D-GalN)/lipopolysaccharide (LPS) in mice. In in vitro experiments, coumarin constituents (hyuganins A-D, anomalin, pteryxin, isopteryxin, and suksdorfin) and polyacetylene constituents [(-)-falcarinol and falcarindiol] substantially inhibited LPS-induced NO and/or TNF-alpha production in mouse peritoneal macrophages, and isoepoxypteryxin inhibited D-GalN-induced cytotoxicity in primary cultured rat hepatocytes. Furthermore, hyuganin A, anomalin, and isopteryxin inhibited the decrease in cell viability by TNF-alpha in L929 cells.
Yoshikawa
Masayuki
M
Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8412, Japan. shoyaku@mb.kyoto-phu.ac.jp
Nishida
Norihisa
N
Ninomiya
Kiyofumi
K
Ohgushi
Teruki
T
Kubo
Mizuho
M
Morikawa
Toshio
T
Matsuda
Hisashi
H
D000114:Acetylene / Q000494:pharmacology*; D029969:Angelica / Q000737:chemistry*; D000818:Animals; D002478:Cells, Cultured; D003374:Coumarins / Q000494:pharmacology*; D005688:Galactosamine / Q000633:toxicity*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects*; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D048069:Tumor Necrosis Factors / Q000096:biosynthesis
D003374:Coumarins; D008070:Lipopolysaccharides; D048069:Tumor Necrosis Factors; D009569:Nitric Oxide; D005688:Galactosamine; D000114:Acetylene