title: The relation between sphingomyelinase activity, lipid peroxide oxidation and NO-releasing in mice liver and brain.
abstract: We used animal models to study connection between oxidating system and sphingomyelin signaling cascade, because this models are more close related to people disease. Activation of n-sphingomyelinase (n-SMase) in mice liver and brain is coincided in time with increased level of peroxide products (conjugated dienes) after injection of tumor necrosis factor alpha (TNF-alpha). We found that ceramide can induce peroxide oxidation and lead to accumulation of TNF-alpha in animal organs. Nitric oxide (NO) donors (S-nitrosoglutathione and dinitrosyl iron complex) reversibly inhibited activity of n-SMase and decreased level of lipid peroxidation products. This data proposed that both SMase and messengers of oxidative systems could be targets for NO-derived oxidants.
authors.affiliation: Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow. ales@sky.chph.ras.ru
authors.forename: A V
authors.initials: AV
authors.lastname: Alessenko
authors.forename: M A
authors.initials: MA
authors.lastname: Shupik
authors.forename: A E
authors.initials: AE
authors.lastname: Bugrova
authors.forename: L B
authors.initials: LB
authors.lastname: Dudnik
authors.forename: L N
authors.initials: LN
authors.lastname: Shingarova
authors.forename: A
authors.initials: A
authors.lastname: Mikoyan
authors.forename: A F
authors.initials: AF
authors.lastname: Vanin
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D002518:Ceramides / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000494:pharmacology; D026422:S-Nitrosoglutathione / Q000494:pharmacology; D013108:Sphingomyelin Phosphodiesterase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology
chemical_list: D002518:Ceramides; D009589:Nitrogen Oxides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; C073661:dinitrosyl iron complex; D007501:Iron; D013108:Sphingomyelin Phosphodiesterase