Efficacy of granulocyte-macrophage colony-stimulating factor on the survival of ischaemic skin flaps in rats.
Our objective was to find out whether granulocyte-macrophage colony-stimulating factor (GM-CSF) improves the survival of skin flaps in rats. Random skin flaps were raised in 30 male Sprague-Dawley rats, and recombinant GM-CSF 50 microg in 0.5 ml saline solution (n=15) or 0.5 ml saline solution alone (n=15) was injected intradermally into the area of the flap after it had been raised. Surviving areas were measured after seven days, and the median area surviving in the group treated with GM-CSF was greater than that in the control group (728 mm2 (range 565-1120) compared with 985 mm2 (range 636-1368), p=0.005). These results suggest that in this model, GM-CSF given postoperatively improves the viability of ischaemic skin flaps.
Kuru
Bekir
B
Department of General Surgery, Ankara Oncology Education and Research Hospital, Turkey. bekirkuru@hotmail.com
Dinc
Soykan
S
Elitok
Orhan
O
Gulcelik
Mehmet Ali
MA
Alagol
Haluk
H
D000818:Animals; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D007511:Ischemia; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D012867:Skin / Q000098:blood supply*; D013524:Surgical Flaps; D014022:Tissue Survival / Q000187:drug effects*
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor