Cell membrane fluidity related to electroporation and resealing.
In this paper, we report the results of a systematic attempt to relate the intrinsic plasma membrane fluidity of three different cell lines to their electroporation behaviour, which consists of reversible and irreversible electroporation. Apart from electroporation behaviour of given cell lines the time course required for membrane resealing was determined in order to distinguish the effect of resealing time from the cell's ability to survive given electric pulse parameters. Reversible, irreversible electroporation and membrane resealing were then related to cell membrane fluidity as determined by electron paramagnetic resonance spectroscopy and computer characterization of membrane domains. We found that cell membrane fluidity does not have significant effect on reversible electroporation although there is a tendency for the voltage required for reversible electroporation to increase with increased membrane fluidity. Cell membrane fluidity, however, may affect irreversible electroporation. Nevertheless, this effect, if present, is masked with different time courses of membrane resealing found for the different cell lines studied. The time course of cell membrane resealing itself could be related to the cell's ability to survive.
Kanduser
Masa
M
Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, Ljubljana, Slovenia.
Sentjurc
Marjeta
M
Miklavcic
Damijan
D
D000818:Animals; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002470:Cell Survival; D003198:Computer Simulation; D006224:Cricetinae; D003412:Cricetulus; D004578:Electron Spin Resonance Spectroscopy; D018274:Electroporation; D005347:Fibroblasts; D006801:Humans; D008168:Lung; D008545:Melanoma; D008560:Membrane Fluidity; D008561:Membrane Fusion; D051379:Mice; D013045:Species Specificity