title: A minimal domain responsible for Munc13 activity.
abstract: Munc13 proteins are essential in neurotransmitter release, controlling the priming of synaptic vesicles to a release-ready state. The sequences responsible for this priming activity are unknown. Here we identify a large alpha-helical domain of mammalian Munc13-1 that is autonomously folded and is sufficient to rescue the total arrest in neurotransmitter release observed in hippocampal neurons lacking Munc13s.
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
authors.forename: Jayeeta
authors.initials: J
authors.lastname: Basu
authors.forename: Nan
authors.initials: N
authors.lastname: Shen
authors.forename: Irina
authors.initials: I
authors.lastname: Dulubova
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.forename: Rong
authors.initials: R
authors.lastname: Guan
authors.forename: Oleg
authors.initials: O
authors.lastname: Guryev
authors.forename: Nick V
authors.initials: NV
authors.lastname: Grishin
authors.forename: Christian
authors.initials: C
authors.lastname: Rosenmund
authors.forename: Josep
authors.initials: J
authors.lastname: Rizo
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D019295:Computational Biology; D019706:Excitatory Postsynaptic Potentials; D005089:Exocytosis / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D017510:Protein Folding; D017433:Protein Structure, Secondary / Q000235:genetics; D017434:Protein Structure, Tertiary; D051381:Rats; D013329:Structure-Activity Relationship; D013395:Sucrose / Q000494:pharmacology
chemical_list: D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; C103912:Unc13a protein, rat; D013395:Sucrose