Divergent HIV and simian immunodeficiency virus surveillance, Zaire.
Recent HIV infection or divergent HIV or simian immunodeficiency virus (SIV) strains may be responsible for Western blot-indeterminate results on 70 serum samples from Zairian hospital employees that were reactive in an enzyme immunoassay. Using universal polymerase chain reaction HIV-1, HIV-2, and SIV primers, we detected 1 (1.4%) HIV-1 sequence. Except for 1 sample, no molecular evidence for unusual HIV- or SIV-like strains in this sampling was found.
Schaefer
Amanda
A
Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Robbins
Kenneth E
KE
Nzilambi
Eugene Nzila
EN
St Louis
Michael E
ME
Quinn
Thomas C
TC
Folks
Thomas M
TM
Kalish
Marcia L
ML
Pieniazek
Danuta
D
D015023:Democratic Republic of the Congo; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D015498:HIV-2 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques; D010564:Personnel, Hospital; D011159:Population Surveillance / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000302:isolation & purification