[The expression of vegf in rat fracture reparative callus].
The present study has determined the histological changes, VEGF (Vascular endothelial growth factor) mRNA alternative expressions and VEGF mRNA levels in chondrogenic-callus, osteogenic-callus and total callus by histology, RT-PCR and Northern blot during rat fracture repair. The results showed that the cellular events of intramembranous ossification, chodrogenesis and endochondral ossification appeared in the callus simultaneously and sequentially. The VEGF mRNAs were expressed in the osteogenic-callus and the chondrogenic-callus, and the strongest expressions were VEGF120 and VEGF164 mRNA. The VEGF mRNA levels in the total callus increased with time after fracture. Take together, these findings suggest that osteogenesis-derived VEGF may play a potential role to modulate the differentiation process of osteogenic and chondrogenic cells during fracture repair.
Li
Chang You
CY
Department of Orthopaedic Surgery, Ist Hospital, China Medical University, Shenyang 110001, China. cyli@mail.cmu.edu.cn
Liang
De Yong
DY
Fan
Guang Yu
GY
Song
Jin Dan
JD
D000818:Animals; D015152:Blotting, Northern; D002146:Bony Callus / Q000378:metabolism*; D050723:Fractures, Bone / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000502:physiology; D042442:Vascular Endothelial Growth Factors / Q000235:genetics* / Q000502:physiology
D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; C467498:vascular endothelial growth factor A, rat