Tangential flow filtration technology applicable to large scale recovery of diphtheria toxin.
The commercial preparation of purified diphtheria toxoid primarily depends on the effective separation of the toxin from a large culture volume of Corynebacterium diphtheriae vaccine strain. Tangential flow or cross-flow filtration is gaining momentum in the processing of biologicals. This study deals with the separation of toxins using a microporous membrane (0.45-microm pore size) with operational variables like transmembrane pressure (TMP), cross flow rate and flux. Under the best conditions, 98% recovery was achieved. The data and conclusions presented here have been drawn from process development work employing available equipment.
Sundaran
Bheeman
B
Pasteur Institute of India, Coonoor 643103, Tamil Nadu, India. piicnr@md4.vsnl.net.in
Palaniappan
Chitrambalam
C
Rao
Yarlagadda Udaya Bhaskara
YU
Boopathy
Ratnam
R
Bhau
Lakshman Narasingha Rao
LN