A shaE deletion mutant showed lower Na+ sensitivity compound to other deletion mutants in the Bacillus subtilis sodium/hydrogen antiporter (Sha) system.
The sodium/hydrogen antiporter (Sha, identical to multiple resistance and pH adaptation: Mrp) encoded by shaABCDEFG is considered to be the major Na+ excretion system in Bacillus subtilis. We constructed deletion mutants of each sha (=mrp) gene and found that a shaE mutant showed lower Na+ sensitivity compared to the other mutants with respect to the growth, sporulation and Na+ efflux activity. ShaE may have a dissimilar role to other Sha proteins in transport functions.
Yoshinaka
Toko
T
Molecular Microbial Functions Division, Bioscience Technology Center, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Takasu
Hirotoshi
H
Tomizawa
Rui
R
Kosono
Saori
S
Kudo
Toshiaki
T