title: Anti-inflammatory activities of the methanol extracts and an isolated furanoditerpene constituent of Sphenocentrum jollyanum Pierre (Menispermaceae).
abstract: Sphenocentrum jollyanum crude extracts and an isolated constituent were evaluated for anti-inflammatory activity using the carrageenan-induced hind paw oedema of healthy adult albino rats and utilizing the oral route of administration. The fruit methanol extract (79.58% inhibition at 200 mg kg(-1)) gave a higher anti-inflammatory activity than the root extract (53.75% inhibition at 200 mg ml(-1)). Further purification of the most active fruit methanol extract (MFE) led to the isolation of three furanoditerpenes identified as columbin, isocolumbin, fibleucin (uv, ir, nmr and ms) as well as a flavonoid-rich fraction (FDE). Both columbin (67.08% inhibition at 20 mg kg(-1), p<0.05) and FDE (76.25% inhibition at 200 mg kg(-1); p<0.05) gave significant anti-inflammatory activities in comparable range with reference acetylsalicylic acid (72.5% inhibition at 100 mg kg(-1)). The results provide some justification for the folkloric uses of Sphenocentrum jollyanum in the treatment of inflammatory-based diseases across the West African sub-region.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria. lanmoody@skannet.com
authors.forename: J O
authors.initials: JO
authors.lastname: Moody
authors.forename: V A
authors.initials: VA
authors.lastname: Robert
authors.forename: J D
authors.initials: JD
authors.lastname: Connolly
authors.forename: P J
authors.initials: PJ
authors.lastname: Houghton
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D004224:Diterpenes / Q000302:isolation & purification / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005638:Fruit; D029583:Menispermaceae; D000432:Methanol / Q000627:therapeutic use*; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use; D018517:Plant Roots; D051381:Rats
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004224:Diterpenes; D010936:Plant Extracts; D000432:Methanol