Spectral unmixing of flavin autofluorescence components in cardiac myocytes.
We applied linear unmixing approach to reveal individual components of intrinsic flavin fluorescence signal recorded in living cardiac cells by spectrally resolved confocal microscopy. Responses of whole-cell autofluorescence to modulators of cell metabolism and respiration were used as a tool of separation of its components; their spectral profiles, estimated by principal component analysis, correspond to free FAD and FAD bound to different enzymes of electron transport chain.
Chorvat
D
D
Department of Biophotonics, International Laser Centre, Ilkovicova 3, 81219 Bratislava, Slovakia. dusan@ilc.sk
Kirchnerova
J
J
Cagalinec
M
M
Smolka
J
J
Mateasik
A
A
Chorvatova
A
A
D000465:Algorithms; D000818:Animals; D019069:Cell Respiration / Q000502:physiology; D002478:Cells, Cultured; D005415:Flavins / Q000378:metabolism*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008164:Luminescent Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005415:Flavins; D008164:Luminescent Proteins