Construction of green fluorescent protein reporter genes for genotoxicity test (SOS/umu-test) and improvement of mutagen-sensitivity.
Green fluorescent protein (GFP) reporter genes for the bacterial umu-test were constructed. Utilization of tandem, lacUV5 and chimeric trp/umu promoters, and coexpression of the Escherichia coli recA5327 mutant enhanced the GFP expression level fourteen-fold over that of the system with only the umu promoter, thereby improving the sensitivity of the umu-test.
Arai
R
R
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Makita
Y
Y
Oda
Y
Y
Nagamune
T
T