Sequence analysis of a beta-agarase gene (pjaA) from Pseudomonas sp. isolated from marine environment.
The pjaA gene of Pseudomonas sp. W7 consists of an open reading frame of 1926 bp encoding beta-agarase, a protein of 642 amino acids and a molecular weight of 69,540 Da. The expressed protein of plasmid pEAG3-3, in which 259 amino acid residues from C-terminus of the overexpression plasmid (pEAG3) were eliminated, led to the complete loss of agarolytic activity.
Lee
S
S
Department of Biotechnology and Bioengineering, Pukyong National University, Pusan 608-737, Korea.
Park
J
J
Yoon
S
S
Kim
J
J
Kong
I
I