Application of electrochemically prepared polypyrrole-polyvinyl sulphonate films to DNA biosensor.
Double stranded calf thymus deoxyribonucleic acid (DNA) was physisorbed onto polypyrrole-polyvinyl sulphonate (PPY-PVS) films electrochemically deposited onto indium-tin-oxide (ITO) coated glass plates. These DNA immobilized PPY-PVS films optimized for various conditions, such as polymerization potential, pH of buffer, DNA concentration and scan rate were characterized using Fourier-transform infrared (FT-IR) spectroscopy, atomic force microscopy (AFM) and cyclic voltammetry (CV) techniques, respectively. The amperometric response studies of these DNA/PPY-PVS electrodes were carried out as a function of 2-aminoantharcene (2-AA, 0.01-20 ppm) and o-chlorophenol (OCP, 0.1-30 ppm) concentration, respectively at 25 degrees C. The observed amperometric current arising due to oxidation of guanine in the DNA/PPY-PVS films decreased linearly with the increase in the concentration of 2-AA and OCP. It has been revealed that 10 ppm of 2-AA is sufficient to reduce the observed guanine oxidation peak current by approximately -95+/-10% as compared to the reported values. A 25 ppm of OCP was capable enough to reduce the guanine oxidation current to zero. These DNA/PPY-PVS electrodes were found to have a shelf life of about 4 months when stored at 25 degrees C.
Arora
Kavita
K
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K.S. Krishnan Road, New Delhi 110012, India.
Chaubey
Asha
A
Singhal
Rahul
R
Singh
Ravindra P
RP
Pandey
M K
MK
Samanta
S B
SB
Malhotra
B D
BD
Chand
Subhash
S
D000873:Anthracenes / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002733:Chlorophenols / Q000032:analysis*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008567:Membranes, Artificial; D009153:Mutagens / Q000032:analysis; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013451:Sulfonic Acids / Q000737:chemistry*
D000873:Anthracenes; D002733:Chlorophenols; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D009153:Mutagens; D011108:Polymers; D011145:Polyvinyls; D011758:Pyrroles; D013451:Sulfonic Acids; C008959:lyapolate; C067635:polypyrrole; C006593:2-anthramine; D004247:DNA