An efficient and versatile solid-phase synthesis of 5'- and 3'-conjugated oligonucleotides.
[reaction: see text] An easy and efficient solid-phase strategy to obtain 5'- and 3'-oligonucleotide conjugates in highly pure form has been developed. Ad hoc derivatized solid supports, to which the first nucleoside unit can be attached through a phosphate linkage, have been exploited both in a pre- and post-DNA assembly conjugation approach. A number of 5'- or 3'- oligonucleotide conjugates, incorporating a variety of labels covalently linked through a phosphodiester or a phosphoramidate bond, have been synthesized and characterized.
D'Onofrio
Jennifer
J
Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico II, Complesso Universitario di Monte S. Angelo, Italy.
Montesarchio
Daniela
D
De Napoli
Lorenzo
L
Di Fabio
Giovanni
G
D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry
D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite