[Research on differential display genes of tolerant-damage induced by trichloroethylene].
OBJECTIVE
To observe the differentially expressed genes of the human normal liver cell (L-02) induced by low concentration of trichloroethylene (TCE).


METHODS
The dose-effect relation of TCE toxicity was analyzed by means of MTT. 5 micromol/L was chosen as low concentration while 40 micromol/L as high concentration. Then L-02 were treated with low concentration, high concentration, pretreated with low concentration then attacked with high concentration of TCE respectively. Fluorescence differential display polymerase chain reaction (Fluoro DD-PCR) was used to search differentially expressed genes of the different treatment groups of TCE. Results 51 differential expressed strap were found, 11 differential straps have been cloned and sequenced. 9 of them were known gene, while 2 of them were new genes.


CONCLUSIONS
The differential straps would be identified to provide scientific base for research deeply the mechanism of low concentration TCE inducing adaptive response of L-02.
Wei
Qin-Zhi
QZ
Department of Health Toxicology, Sun Yat-sen Medical University, Guangzhou 510080, China.
Zhuang
Zhi-Xiong
ZX
Yuan
Jian-Hui
JH
Huang
Hai-Yan
HY
Li
Xiyi
X
Yang
Xiaohua
X
Geng
Lei
L
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D014241:Trichloroethylene / Q000633:toxicity*
D014241:Trichloroethylene