title: [Identification of up-regulated genes induced by angiotensin II in cardiac fibroblasts].
abstract: To identify up-regulated genes in adult rat cardiac fibroblasts (CF) induced by angiotensin II (Ang II), suppression subtractive hybridization (SSH) was performed between the CF stimulated by Ang II (tester) and unstimulated CF (driver) to generate subtractive cDNA library. The library was screened with dot blots hybridization to further verify the differentially expressed cDNA clones. Partial positive clones (19 up-regulated genes) were sequenced and BLAST analyzed. Twelve up-regulated genes related to extracellular matrix, cell cycle, intracellular signal transduction, cell cytoskeleton, cell metabolism and 7 new expressed sequence tags (EST) were acquired (GenBank accession number: CN382808, CN382809, CN382810, CN382811, CN382812, CN382813, CN382814). Our data reveal that SSH is a powerful technique of high sensitivity for the detection and cloning of up-regulated genes expressed in CF induced by Ang II, which may be helpful to clarify the mechanism of cardiac remodeling.
authors.affiliation: Department of Pathophysiology, Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Xin-Feng
authors.initials: XF
authors.lastname: Wang
authors.forename: Guang-Dao
authors.initials: GD
authors.lastname: Gao
authors.forename: Yu-Bai
authors.initials: YB
authors.lastname: Yang
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Wang
authors.forename: Xing-Li
authors.initials: XL
authors.lastname: Su
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Feng-Chan
authors.initials: FC
authors.lastname: Han
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Bai
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D005347:Fibroblasts / Q000166:cytology*; D005786:Gene Expression Regulation; D008297:Male; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D020257:Ventricular Remodeling / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D000804:Angiotensin II