title: Selective activation of adrenergic beta1 receptors induces heme oxygenase 1 production in RAW264.7 cells.
abstract: We hypothesized that catecholamines through beta-adrenoceptor might modulate macrophage function. We showed that isoproterenol concentration-dependently induced HO-1 production through beta(1)-but not beta(2)-adrenoceptor. Production was increased by forskolin and inhibited by pretreatment with the PKA inhibitor, H-89. Furthermore, induction of HO-1 by isoproterenol effectively protected RAW264.7 cells from effects of glucose oxidase treatment, which was abrogated either by HO-1 inhibitor, ZnPP IX and beta-adenoceptor antagonist, propranolol. Thus, stimulation of HO-1 production through beta(1)-adenoceptors, and via the PKA pathways by isoproterenol, can enable RAW264.7 cells to resist oxidant stress, suggesting that catecholamine hormones may be necessary, at least, to maximize defending role of macrophages.
authors.affiliation: Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Jinji
authors.initials: J
authors.lastname: Sun
authors.forename: Sung Jae
authors.initials: SJ
authors.lastname: Kim
authors.forename: Min Kyu
authors.initials: MK
authors.lastname: Park
authors.forename: Hye Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Irina
authors.initials: I
authors.lastname: Tsoy
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Kang
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Lee
authors.forename: Han Geuk
authors.initials: HG
authors.lastname: Seo
authors.forename: Jae Heun
authors.initials: JH
authors.lastname: Lee
authors.forename: Ki Churl
authors.initials: KC
authors.lastname: Chang
mesh_terms: D058665:Adrenergic beta-1 Receptor Agonists; D058671:Adrenergic beta-1 Receptor Antagonists; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D015870:Gene Expression / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D007545:Isoproterenol / Q000494:pharmacology; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D011524:Protoporphyrins / Q000494:pharmacology; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics
chemical_list: D058665:Adrenergic beta-1 Receptor Agonists; D058671:Adrenergic beta-1 Receptor Antagonists; D000318:Adrenergic beta-Agonists; D002395:Catecholamines; D011524:Protoporphyrins; D018342:Receptors, Adrenergic, beta-1; C017803:zinc protoporphyrin; D005576:Colforsin; D051547:Heme Oxygenase-1; D017868:Cyclic AMP-Dependent Protein Kinases; D007545:Isoproterenol