Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes.
The effect of glycosaminoglycan addition on a three-dimensional (3D) culture of porcine chondrocyte cells was investigated with a view to use in cartilage regenerative medicine. Chondroitin sulfate C increased the mRNA expression of type 2 collagen, while chondroitin sulfate A did not. Hyaluronic acid of high molecular weight markedly decreased the mRNA expression of both aggrecan and type 2 collagen, although hyaluronic acid of low molecular weight showed no apparent effect.
Nishimoto
Shohei
S
International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Takagi
Mutsumi
M
Wakitani
Shigeyuki
S
Nihira
Takuya
T
Yoshida
Toshiomi
T
D053674:Aggrecans; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism*; D002809:Chondroitin Sulfates / Q000008:administration & dosage* / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000737:chemistry; D024043:Collagen Type II / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D037181:Lectins, C-Type; D008422:Materials Testing; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D011509:Proteoglycans / Q000096:biosynthesis*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
D053674:Aggrecans; D020099:Coated Materials, Biocompatible; D024043:Collagen Type II; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011108:Polymers; D011509:Proteoglycans; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates