[Effect of high glucose on the expression of fibronectin and plasminogen activator inhibitor-1 in human peritoneal mesothelial cells].
OBJECTIVE
To evaluate the effect of high glucose on the expression of fibronectin (FN) and plasminogen activator inhibitor-1 (PAI-1) in human peritoneal mesothelial cells (HPMC).


METHODS
The cultured HPMC were exposed to the culture medium containing different concentrations of glucose (1.5%, 2.5%, 4.25%) for 6, 12, 24, 48 hours, and normal culture medium was used as control. FN and PAI-1 in supernatant were detected by enzyme-linked immunoassay(ELISA).


RESULTS
The glucose at 1. 5%, 2.5%, 4.25% resulted in a time- and concentration-dependent increase in FN expression in HPMC(P < 0.05). The glucose at 2.5%, 4.25% increased PAI-1 expression in HPMC (P < 0.05), and the effect was time- and concentration-dependent. PAI-1 expression was not affected by 1.5% glucose (P > 0.05).


CONCLUSIONS
High glucose increases the expression of FN and PAI-1 in HPMC, thus playing a part in the progression of peritoneal fibrosis.
Ji
Ling
L
Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhuang
Bin
B
Yang
Li-Chuan
LC
Liu
Xiao-Jing
XJ
Liu
Xian-Rong
XR
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D010530:Peritoneal Dialysis / Q000009:adverse effects; D010537:Peritoneum / Q000166:cytology* / Q000378:metabolism; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis*
D005353:Fibronectins; D017395:Plasminogen Activator Inhibitor 1; D005947:Glucose