title: Inhibition of store-operated calcium entry-mediated superoxide generation by histamine trifluoromethyltoluide independent of histamine receptors.
abstract: Store-operated calcium entry (SOCE) plays an important role in shaping the Ca(2+) response of various tissues and cell types. In this report, we show that thapsigargin (TG)-induced SOCE was inhibited by the histamine receptor agonist, histamine-trifluoromethyltoluide (HTMT), in U937 and HL-60 human promyelocytes. Preincubation of HTMT resulted in a significant inhibition of subsequent TG-induced Ca(2+) elevation without affecting Ca(2+) release from intracellular stores. HTMT also inhibited TG-induced Ca(2+) current and Ba(2+)/Mn(2+) influx in a concentration-dependent manner. In contrast with HTMT, other H1 histamine receptor agonists, histamine, 2-methylhistamine and 2-thiazolylethylamine, did not affect TG-induced SOCE. In addition, HTMT also attenuated TG-induced cytosolic superoxide generation. Taken together, our data clearly suggest that the anti-inflammatory effect of HTMT may occur through direct inhibition of SOCE.
authors.affiliation: Division of Molecular and Life Science, SBD-NCRC, Pohang University of Science and Technology, Pohang 790-784, POSTECH, San 31, Hyoja Dong, South Korea.
authors.forename: Dong-Chan
authors.initials: DC
authors.lastname: Kim
authors.forename: So-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Dong-Jae
authors.initials: DJ
authors.lastname: Jun
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Jong-Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Hur
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
authors.forename: Kyong-Tai
authors.initials: KT
authors.lastname: Kim
mesh_terms: D002118:Calcium / Q000378:metabolism*; D018922:HL-60 Cells; D006632:Histamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006633:Histamine Antagonists / Q000494:pharmacology*; D006801:Humans; D011968:Receptors, Histamine / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D020298:U937 Cells
chemical_list: D006633:Histamine Antagonists; D011968:Receptors, Histamine; C087869:histamine trifluoromethyl-toluidide; D013481:Superoxides; D006632:Histamine; D002118:Calcium