A blue fluorescent antibody-cofactor sensor for mercury.
[reaction: see text] A chemically programmed antibody sensor, consisting of a stilbenyl boronic acid cofactor and monoclonal antibody EP2-19G2, provides a new method of mercury detection. The fluorescent antibody sensor generates an intense powder blue fluorescence when bound to the stilbenyl boronic acid cofactor; however, it is quenched in the presence of Hg(2+) ions. The EP2-19G2-cofactor biosensor provides micromolar sensitivity and selectivity toward Hg(2+) ions over a wide range of metal ions in aqueous solution.
Matsushita
Masayuki
M
Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA. kdjanda@scripps.edu
Meijler
Michael M
MM
Wirsching
Peter
P
Lerner
Richard A
RA
Janda
Kim D
KD
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D015374:Biosensing Techniques; D001897:Boronic Acids / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D008956:Models, Chemical; D015394:Molecular Structure; D013267:Stilbenes / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000911:Antibodies, Monoclonal; D001897:Boronic Acids; D005456:Fluorescent Dyes; D013267:Stilbenes; D014867:Water; D008628:Mercury