Differential mRNA stability to endogenous ribonucleases of the coding region and 3' untranslated regions of wheat (Triticum aestivum L.) manganese superoxide dismutase genes.
The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenous nucleases. The degradation rates of the 3' UTR variants for 15 min were not significantly different, meaning the degradation rates of the 3' UTR variants were not directly related to the thermostabilities. However, the degradation rate of the coding region was significantly faster than those of the 3' UTR variants. Further investigation revealed the coding region seemed to have specific sites for degradation, indicating a possibility of increasing MnSOD expression by the degradation site alteration.
Baek
Kwang-Hyun
KH
Department of Crop and Soil Sciences, Washington State University, WA 99164-6420, USA.
Skinner
Daniel Z
DZ
D020413:3' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics*; D020871:RNA Stability; D012260:Ribonucleases / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics*; D014908:Triticum / Q000201:enzymology* / Q000235:genetics*
D020413:3' Untranslated Regions; D018076:DNA, Complementary; D013482:Superoxide Dismutase; D012260:Ribonucleases