[Studies on the determination of protein with spectroscopic probe of o-chlorophenylfluorone-molybdenum (VI) complex in microemulsion medium].
The interaction and absorption spectral behavior of o-chlorophenylfluorone (o-CPF)-Mo(VI) and protein are studied in OP microemulsion medium. Under the optimum condition, the molar absorptivity of o-CPF-Mo(VI)-BSA system at 532 nm is 6.12 x 10(6) L x mol(-1) x cm(-1), and Beer's law is obeyed in the range of 0-14 microg x mL(-1) of BSA. Moreover, the binding number between the complex and BSA determined by molar ratio and slope ratio methods is 91. The mechanism of interaction of BSA and o-CPF-Mo (VI) is also discussed. OP microemulsion media introduced into the protein determination increased markedly the sensitivity of the system. The result of experiment indicates that the method possesses a high sensitivity as well as a high selectivity. It can be used to determine protein in human urine with satisfactory results.
Du
Bin
B
School of Chemistry and Chemical Engineering, Ji'nan University, Ji'nan 250022, China.
Li
Yan
Y
Zhang
Hui
H
Wu
Dan
D
Wei
Qin
Q
Li
Zai-jun
ZJ
D004655:Emulsions; D005452:Fluoresceins / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008982:Molybdenum / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D011507:Proteinuria / Q000652:urine; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*
D004655:Emulsions; D005452:Fluoresceins; D009942:Organometallic Compounds; D011506:Proteins; C010159:fluorone Black; D008982:Molybdenum