title: Helianthus tuberosus agglutinin directly induces neutrophil migration, which can be modulated/inhibited by resident mast cells.
abstract: We investigated the effect of Helianthus tuberosus agglutinin (HTA) on neutrophil migration in vivo and in vitro. The role of resident cells in this effect was analyzed. Peritonitis was induced by injecting stimuli into rat (150-200 g) peritoneal cavities, and in vitro neutrophil chemotaxis was performed using a Boyden microchamber. HTA (80, 200, or 500 microg/mL per cavity) induced significant in vivo neutrophil migration (p < 0.05); in vitro assays showed that this lectin also induced neutrophil chemotaxis, an effect inhibited by the incubation of lectin associated with alpha-D(+)-mannose, its specific binding sugar. Depletion of the resident-cell population by peritoneal lavage did not alter HTA-induced neutrophil migration (200 microg/mL per cavity). The opposite strategy, increasing peritoneal macrophages by intraperitoneally injecting rats with thioglycollate, did not enhance the neutrophil migration produced by HTA (200 microg/mL per cavity). In addition, injection of supernatant from HTA-stimulated macrophage culture (300 microg/mL) into rat peritoneal cavities did not induce neutrophil migration. However, reduction of the peritoneal mast-cell population potentiated the neutrophil migration (p < 0.05) induced by HTA (200 microg/mL per cavity). Lectin from H. tuberosus has a direct neutrophil chemotatic effect that is modulated by mast cells.
authors.affiliation: Dept. de Fisiologia e Farmacologia/Faculdade de Medicina, UniversidadeFederal do Ceará, Rua Coronel Nunes de Melo, 1127 Rodolfo Teófilo 60430-270, Fortaleza, Ceará, Brazil.
authors.forename: Veruska B M
authors.initials: VB
authors.lastname: Alencar
authors.forename: Gerly A C
authors.initials: GA
authors.lastname: Brito
authors.forename: Nylane M N
authors.initials: NM
authors.lastname: Alencar
authors.forename: Ana M S
authors.initials: AM
authors.lastname: Assreuy
authors.forename: Vicente P T
authors.initials: VP
authors.lastname: Pinto
authors.forename: Edson H
authors.initials: EH
authors.lastname: Teixeira
authors.forename: Emmanuel P
authors.initials: EP
authors.lastname: Souza
authors.forename: Henri
authors.initials: H
authors.lastname: Debray
authors.forename: Ronaldo A
authors.initials: RA
authors.lastname: Ribeiro
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000166:cytology*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D037121:Plant Lectins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C117435:Helianthus tuberosus lectin; D037121:Plant Lectins