title: Gamma-linolenic acid enrichment from Borago officinalis and Echium fastuosum seed oils and fatty acids by low temperature crystallization.
abstract: Solvent winterization of seed oil and free fatty acids (FFAs) was employed to obtain gamma-linolenic acid (GLA; 18:3omega6) concentrates from seed oils of two Boraginaceae species, Echium fastuosum and Borago officinalis. Different solutions of seed oils and FFAs from these two oils at 10%, 20% and 40% (w/w) were crystallized at 4 degrees C, -24 degrees C and -70 degrees C, respectively, using hexane, acetone, diethyl ether, isobutanol and ethanol as solvents. Best results were obtained for B. officinalis FFAs in hexane, reaching a maximum GLA concentration of 58.8% in the liquid fraction (LF). In E. fastuosum, the highest GLA concentration (39.9%) was also achieved with FFAs in hexane.
authors.affiliation: Area de Tecnología de Alimentos, Departamento de Ingeniería Química, Universidad de Almería, 04120 Almería, Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: López-Martínez
authors.forename: Pablo
authors.initials: P
authors.lastname: Campra-Madrid
authors.forename: José Luis
authors.initials: JL
authors.lastname: Guil-Guerrero