title: [Expression of Nestin following focal cerebral ischemia-reperfusion in rats].
abstract: OBJECTIVE
  To study the expession of Nestin after focal ischemia-reperfusion in rats.
  
  
  METHODS
  The model of acute reperfusion injury after cerebral ischemia in rat was made by middle cerebral artery occlusion. All animals were allowed 2 h ischemia and sacrificed after different durations of reperfusion (6 h, 12 h, 24 h, 72 h, 7 d, 14 d). The expression of Nestin was detected with an immunohistochemical approach.
  
  
  RESULTS
  The number of Nestin- immunoreactive cells at 6 h after reperfusion in the ischemic side started to increase markedly and showed significant difference, compared with the Sham group (P < 0.05). The expression of Nestin and the number of Nestin positive cells with Brdu labeled peaked at 7 d after reperfusion and then were down-regulated at 14 d.
  
  
  CONCLUSIONS
  The study implies cerebral ischemia could upregulate the expression of Nestin and induce the proliferation of nervous stem cells.
authors.affiliation: Department of Pathology, Chengdu Medical College, Chengdu 610083, China.
authors.forename: Xiao-Song
authors.initials: XS
authors.lastname: Hu
authors.forename: De-Ming
authors.initials: DM
authors.lastname: Zhou
authors.forename: Dong
authors.initials: D
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D064231:Nestin; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin