An instant measurement of oxidoreductase activity above 100 degrees C by monitoring the absorbance change.
A conventional absorbance monitoring method using a cuvette covered with a tight rubber cap was found to be applicable for measuring oxidoreductase activity at temperatures up to 115 degrees C. Using this method, the optimal temperatures of the enzymes, including oxygen-sensitive enzymes from a hyperthermophilic archaeon Thermococcus profundus, were determined.
Imai
Takeo
T
Department of Life Science, Graduate School of Life Science, Rikkyo (St. Paul's) University, 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan. imaitak@rikkyo.ac.jp
Yasujima
Daisuke
D
Siddiqui
Masood Ahmed
MA