Human protein tau represses DNA replication in vitro.
Here, in the experiments of both PCR and real-time PCR, a repression of DNA amplification was observed in the presence of protein tau. Furthermore, a strong repression appeared when an in vitro DNA replication assay was performed at the physiological temperature (37 degrees C). The incorporation of dNTP was markedly decreased to approximately 12% of control by the presence of tau23 and to approximately 15% by tau40. In the competitive experiments, the PCR product could be restored when the competitor DNA was added, indicating that the association of tau with the template gave rise to the repression. However, tau did not repress the yield of RNA in transcription, suggesting that tau was replaced or ejected from the template by the elongating T7 RNA polymerase.
Li
Wen
W
The State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China.
Wang
Xing Sheng
XS
Qu
M H
MH
Liu
Ying
Y
He
Rong Qiao
RQ
D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D006801:Humans; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic; D016875:tau Proteins / Q000378:metabolism*
D016875:tau Proteins; D004247:DNA; D019914:Taq Polymerase