title: Chiral separation of halogenated amino acids by ligand-exchange capillary electrophoresis.
abstract: The chiral separation of halogenated amino acids by ligand-exchange CE is described. Halogenated amino acids attracted increasing interest in recent years because of their physiological activities. Different chiral selectors, as there are L-4-hydroxyproline, L-histidine, and N-alkyl derivatives of L-4-hydroxyproline in form of their copper(II) complexes, are compared for their chiral recognition ability for halogenated amino acids. The influence of various parameters, such as selector concentration, pH, organic modifier, and field strength, on the resolution was investigated. All halogenated amino acids investigated were baseline-separated under optimized conditions.
authors.affiliation: Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, Karl-Franzens-University, Graz, Austria.
authors.forename: Julia
authors.initials: J
authors.lastname: Koidl
authors.forename: Heike
authors.initials: H
authors.lastname: Hödl
authors.forename: Martin G
authors.initials: MG
authors.lastname: Schmid
authors.forename: Svetlana
authors.initials: S
authors.lastname: Pantcheva
authors.forename: Tamara
authors.initials: T
authors.lastname: Pajpanova
authors.forename: Gerald
authors.initials: G
authors.lastname: Gübitz
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification*; D003300:Copper; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D006219:Halogens; D006639:Histidine / Q000302:isolation & purification; D006909:Hydroxyproline / Q000302:isolation & purification; D008024:Ligands; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D006219:Halogens; D008024:Ligands; D006639:Histidine; D003300:Copper; D006909:Hydroxyproline