A convenient method for preparation of the calcium ion-binding protein annexin V.
This study presents a novel method for the production and purification of annexin V using cation exchange chromatography in the presence of calcium ions at neutral or alkaline pH. This method enables the handling of a large quantity of sample at one time without deterioration of the adsorption capacity of the cation exchange carrier by contaminating proteins, as well as the production of the calcium ion-binding protein annexin V with high purity on an industrial scale from both recombinant products and native products.
Mizokami
Hiroshi
H
Applied Research Department, The Chemo-Sero-Therapeutic Research Institute (Kaketsuken), Kyokushi, Kikuchi, Kumamoto 869-1298, Japan.
Furukawa
Shinichi
S
Sugawara
Keishin
K
Maeda
Toshihiro
T
Onchi
Tatsufumi
T
Soejima
Kenji
K
Koyanagi
Satoshi
S
Yoshizaki
Hideo
H
Hirayama
Chuichi
C