title: Proteomics of organelles and large cellular structures.
abstract: The mass-spectrometry-based identification of proteins has created opportunities for the study of organelles, transport intermediates and large subcellular structures. Traditional cell-biology techniques are used to enrich these structures for proteomics analyses, and such analyses provide insights into the biology and functions of these structures. Here, we review the state-of-the-art proteomics techniques for the analysis of subcellular structures and discuss the biological insights that have been derived from such studies.
authors.affiliation: Department of Cell Biology, 10550 North Torrey Pines Road, The Scripps Research Institute, La Jolla, California 92037, USA. jyates@scripps.edu
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Annalyn
authors.initials: A
authors.lastname: Gilchrist
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Howell
authors.forename: John J M
authors.initials: JJ
authors.lastname: Bergeron
mesh_terms: D002458:Cell Fractionation; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D015388:Organelles / Q000737:chemistry* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D020543:Proteome