title: Hydrogenase genes are uncommon and highly conserved in Rhizobium leguminosarum bv. viciae.
abstract: A screening for hydrogen uptake (hup) genes in Rhizobium leguminosarum bv. viciae isolates from different locations within Spain identified no Hup+ strains, confirming the scarcity of the Hup trait in R. leguminosarum. However, five new Hup+ strains were isolated from Ni-rich soils from Italy and Germany. The hup gene variability was studied in these strains and in six available strains isolated from North America. Sequence analysis of three regions within the hup cluster showed an unusually high conservation among strains, with only 0.5-0.6% polymorphic sites, suggesting that R. leguminosarum acquired hup genes de novo in a very recent event.
authors.affiliation: Laboratorio de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria, s/n, 28040 Madrid, Spain.
authors.forename: Domingo
authors.initials: D
authors.lastname: Fernández
authors.forename: Annita
authors.initials: A
authors.lastname: Toffanin
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Palacios
authors.forename: Tomás
authors.initials: T
authors.lastname: Ruiz-Argüeso
authors.forename: Juan
authors.initials: J
authors.lastname: Imperial
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000235:genetics; D019143:Evolution, Molecular; D007887:Fabaceae / Q000382:microbiology; D005798:Genes, Bacterial; D006864:Hydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids / Q000235:genetics; D011110:Polymorphism, Genetic; D016961:Rhizobium leguminosarum / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D006864:Hydrogenase