title: Multicommuted flow-through fluorescence optosensor for determination of furosemide and triamterene.
abstract: Multicommutation implemented with flow-through optosensors is a very promising area of research. This recent approach benefits from the advantages of both methods and results in high sensitivity, selectivity, and speed, and little waste generation. This paper reports the simultaneous determination of furosemide and triamterene, two widely used diuretics, by measurement of their native fluorescence. The system has been proved to be useful for determination of both analytes in pharmaceutical preparations and for determination of triamterene in human urine and serum. A minicolumn filled with Sephadex SPC-25 microbeads was used to achieve separation of both analytes before detection in a flow-through cell filled with the same resin. The sensor is linear in the range 50-1200 and 0.4-8 ng mL(-1) with detection limits of 15 and 0.1 ng mL(-1) for furosemide and triamterene, respectively.
authors.affiliation: Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Paraje Las Lagunillas, 23071 Jaén, Spain.
authors.forename: E J
authors.initials: EJ
authors.lastname: Llorent-Martínez
authors.forename: P
authors.initials: P
authors.lastname: Ortega-Barrales
authors.forename: A
authors.initials: A
authors.lastname: Molina-Díaz
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D004232:Diuretics / Q000032:analysis; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005665:Furosemide / Q000032:analysis / Q000097:blood* / Q000652:urine*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics / Q000295:instrumentation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D014159:Transducers; D014223:Triamterene / Q000032:analysis / Q000097:blood* / Q000652:urine*; D016482:Urinalysis / Q000295:instrumentation* / Q000379:methods
chemical_list: D004232:Diuretics; D005665:Furosemide; D014223:Triamterene