title: Cloning of full-length cDNA sequence of a novel candidate gene related to mouse cleft palate.
abstract: OBJECTIVE
  Cloning and screening a novel candidate gene related to developing mouse cleft palate.
  
  
  METHODS
  The differentially expressed genes were cloned by modified PCR-based subtractive hybridization. After identification using reverse dot blotting, positive clones were sequenced and analyzed for homology in GenBank databases.
  
  
  RESULTS
  Four novel express sequence tags were obtained, one of which spanning 809 bp was the full-length of the novel gene cDNA identified by Northern hybridization.
  
  
  CONCLUSIONS
  A novel candidate gene related to mouse cleft palate was cloned.
authors.affiliation: Department of Oral Pathology, Stomatology College, the Fourth Military Medical University, Xi'an, Shaanxi, 710032 PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xin-wen
authors.initials: XW
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002972:Cleft Palate / Q000235:genetics* / Q000473:pathology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D017422:Sequence Analysis, DNA
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018076:DNA, Complementary; C515627:MCPR1 protein, mouse