title: Application of high-performance anion-exchange chromatography with pulsed amperometric detection and statistical analysis to study oligosaccharide distributions--a complementary method to investigate the structure and some properties of alginates.
abstract: Alginates comprised of essentially alternating units of mannuronic (M) acid-guluronic (G) acid (MG-alginate), and G-blocks isolated from a seaweed where subjected to partial acid hydrolysis at pH 3.5 The chain-length distribution of oligosaccharides in the hydrolysate were investigated by statistical analysis after their separation with high-performance anion-exchange chromatography and pulsed amperometric detection (HPAEC-PAD). Simulated depolymerisation of the MG-alginate provided an estimate of the ratio between two acid hydrolysis rate constants (p=8.3+/-1) and the average distribution of the MM linkages in the original sample of polysaccharide chains. In conclusion, we found HPAEC-PAD together with statistical analysis was a useful method to investigate the fine structure and some properties of binary polysaccharides.
authors.affiliation: Norwegian Biopolymer Laboratory (NOBIPOL), Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Sem Saelands vei 6/8, N-7491 Trondheim, Norway. simon.ballance@biotech.ntnu.no
authors.forename: Simon
authors.initials: S
authors.lastname: Ballance
authors.forename: Synnøve
authors.initials: S
authors.lastname: Holtan
authors.forename: Olav Andreas
authors.initials: OA
authors.lastname: Aarstad
authors.forename: Pawel
authors.initials: P
authors.lastname: Sikorski
authors.forename: Gudmund
authors.initials: G
authors.lastname: Skjåk-Braek
authors.forename: Bjørn E
authors.initials: BE
authors.lastname: Christensen
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000837:Anion Exchange Resins; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*
chemical_list: D000464:Alginates; D000837:Anion Exchange Resins; D009844:Oligosaccharides