Bilateral lavage with diluted surfactant improves lung function after unilateral lung contusion in pigs.
OBJECTIVE
This study evaluates the effects of bronchoalveolar lavage with diluted surfactant on unilateral lung contusion-induced lung dysfunction.


METHODS
Randomized prospective animal study.


METHODS
An animal laboratory.


METHODS
Twenty adult pigs, weighing 25-35 kg.


METHODS
Animals were randomly assigned to controls and surfactant treatment. Bilateral lavage with surfactant treatment began 30 mins after unilateral lung contusion. Then 25 mg/kg of body weight diluted Curosurf (5 mg/mL) was applied in a volume of 5 mL/kg of body weight. Observation time was 8 hrs postinjury.


RESULTS
The Pao2/Fio2 ratio fell from 500 to 250 and then recovered gradually in controls and surfactant-treated pigs. After another 4 hrs, the Pao2/Fio2 ratio deteriorated again in controls, but not in surfactant-treated animals. Total compliance fell by 50% after injury but was completely restored by surfactant treatment. Lung contusion increased the median number of neutrophils in bronchoalveolar lavage fluid from 2% to 30% of total cells and peaked >60% at 480 mins in the contused lungs of control pigs. Surfactant-treated pigs had 40% neutrophils at 480 mins without reaching significant difference to controls. The leukocyte neutral proteinase inhibitor increased to 500 ng/mL at 30 mins postinjury in the contused lungs and increased to 2000 ng/mL after surfactant treatment.


CONCLUSIONS
Bilateral bronchoalveolar lavage with diluted surfactant can effectively improve lung function after experimental unilateral lung contusion in pigs.
Strohmaier
Wolfgang
W
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse13, 1200 Vienna, Austria.
Trupka
Arnold
A
Pfeiler
Claudia
C
Thurnher
Martin
M
Khakpour
Zafar
Z
Gippner-Steppert
Cornelia
C
Jochum
Marianne
M
Redl
Heinz
H
D000818:Animals; D001688:Biological Products / Q000008:administration & dosage*; D001794:Blood Pressure / Q000502:physiology; D018893:Bronchoalveolar Lavage / Q000379:methods*; D002302:Cardiac Output / Q000502:physiology; D003288:Contusions / Q000503:physiopathology / Q000628:therapy*; D008168:Lung / Q000503:physiopathology; D055370:Lung Injury; D010743:Phospholipids / Q000008:administration & dosage*; D011663:Pulmonary Surfactants / Q000008:administration & dosage*; D012129:Respiratory Function Tests; D012965:Sodium Chloride / Q000008:administration & dosage*; D013552:Swine
D001688:Biological Products; D010743:Phospholipids; D011663:Pulmonary Surfactants; D012965:Sodium Chloride; C068291:poractant alfa