title: A multilayer membrane amperometric glucose sensor fabricated using planar techniques for large-scale production.
abstract: This paper reports on a multilayer membrane amperometric glucose sensor fabricated using planar techniques. It is characterized by good reproducibility and suitable for large-scale production. The glucose sensor has 82 electrode sets formed on a single glass substrate, each with a platinum working electrode (WE), a platinum counter electrode (CE) and an Ag/AgCl reference electrode (RE). The electrode sets are coated with a membrane consisting of five layers: gamma-aminopropyltriethoxysilane (gamma-APTES), Nafion, glucose oxidase (GOX), gamma-APTES and perfluorocarbon polymer (PFCP), in that order. Tests have shown that the sensor has acceptably low dispersion (relative standard deviation, R.S.D.=42.9%, n=82), a wide measurement range (1.11-111 mM) and measurement stability over a 27-day period. Measurements of the glucose concentration in a control human urine sample demonstrated that the sensor has very low dispersion (R.S.D.=2.49%, n=10).
authors.affiliation: Fundamental and Environmental Research Laboratories, NEC Corporation, 34 Miyukigaoka, Tsukuba, Ibaraki 305-8501, Japan. t-matsumoto@dt.jp.nec.com
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: S
authors.initials: S
authors.lastname: Saito
authors.forename: S
authors.initials: S
authors.lastname: Ikeda
mesh_terms: D015374:Biosensing Techniques; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry; D005466:Fluorocarbons / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D008567:Membranes, Artificial; D010984:Platinum / Q000737:chemistry; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D014556:Urine / Q000737:chemistry
chemical_list: D005465:Fluorocarbon Polymers; D005466:Fluorocarbons; D008567:Membranes, Artificial; D011437:Propylamines; D012821:Silanes; C040402:perfluorosulfonic acid; D010984:Platinum; D005949:Glucose Oxidase; D005947:Glucose; C477625:amino-propyl-triethoxysilane