title: DADLE induces a reversible hibernation-like state in HeLa cells.
abstract: [D-Ala(2)-D-Leu(5)-Enkephalin] (DADLE) can induce hibernation when injected into ground squirrels in summer and is able to increase the survival time of explanted organs such as liver and lung. Since cell metabolism is a target of this peptide, we have treated HeLa cells with DADLE and investigated its possible effect on transcription and proliferation as well as the resumption of metabolic activity after treatment. The labelling for Pol I, Pol II and for splicing factors such as snRNPs and SC-35 decreased after treatment as did the nucleolar labelling for UBF. In treated cells, several spherical nuclear bodies were found to be labelled for hnRNPs. In parallel, the number of proliferating cells decreased after treatment with DADLE. After recovery, there was a gradual resumption of cell function: transcription and splicing factors had a distribution similar to that of controls; proliferation resumed; nuclear bodies, representing storage sites for RNPs, disappeared.
authors.affiliation: Laboratorio di Biologia Cellulare e Neurobiologia, Dipartimento di Biologia Animale, Università di Pavia and Istituto di Genetica Molecolare del CNR, Piazza Botta 10, 27100, Pavia, Italy.
authors.forename: Lorella
authors.initials: L
authors.lastname: Vecchio
authors.forename: Cristiana
authors.initials: C
authors.lastname: Soldani
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Bottone
authors.forename: Manuela
authors.initials: M
authors.lastname: Malatesta
authors.forename: Terence E
authors.initials: TE
authors.lastname: Martin
authors.forename: Lawrence I
authors.initials: LI
authors.lastname: Rothblum
authors.forename: Carlo
authors.initials: C
authors.lastname: Pellicciari
authors.forename: Marco
authors.initials: M
authors.lastname: Biggiogera
mesh_terms: D000963:Antimetabolites / Q000378:metabolism; D015153:Blotting, Western; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D016308:Enkephalin, Leucine-2-Alanine / Q000494:pharmacology*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D012334:RNA, Neoplasm / Q000096:biosynthesis; D020394:Vault Ribonucleoprotein Particles / Q000187:drug effects
chemical_list: D000963:Antimetabolites; D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D012334:RNA, Neoplasm; D020394:Vault Ribonucleoprotein Particles; D016308:Enkephalin, Leucine-2-Alanine; D001973:Bromodeoxyuridine; D016650:Fluorescein-5-isothiocyanate