[Effect of DNA-damaging agents on the aerobic methylobacteria capable and incapable of utilizing dichloromethane].
Methylobacterium dichloromethanicum DM4, a degrader of dichloromethane (DCM), was more tolerant to the effect of H2O2 and UV irradiation than Methylobacterium extorquens AM1, which does not consume DCM. Addition of CH2Cl2 to methylobacteria with active serine, ribulose monophosphate, and ribulose bisphosphate pathways of C1 metabolism, grown on methanol, resulted in a 1.1- to 2.5-fold increase in the incorporation of [alpha-32P]dATP into DNA Klenow fragment (exo-). As DCM dehalogenase was not induced in this process, the increase in total lengths of DNA gaps resulted from the action of DCM rather than S-chloromethylglutathione (intermediate of primary dehalogenation). The degree of DNA damage in the presence of CH2Cl2 was lower in DCM degraders than methylobacteria incapable of degrading this pollutant. This suggests that DCM degraders possess a more efficient mechanism of DNA repair.
Firsova
Iu E
IuE
Torgonskaia
M L
ML
Doronina
N V
NV
Trotsenko
Iu A
IuA
D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008190:Lyases / Q000378:metabolism; D000432:Methanol; D008752:Methylene Chloride / Q000378:metabolism* / Q000494:pharmacology; D020580:Methylobacterium / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D014466:Ultraviolet Rays
D003470:Culture Media; D004269:DNA, Bacterial; D008752:Methylene Chloride; D006861:Hydrogen Peroxide; D008190:Lyases; C045224:dichloromethane dehalogenase; D000432:Methanol