QCM-D studies of attachment and differential spreading of pre-osteoblastic cells on Ta and Cr surfaces.
The quartz crystal microbalance with dissipation (QCM-D) technique was employed to characterize initial cell adhesion in terms of attachment and spreading of pre-osteoblastic MC3T3-E1 cells on Ta and Cr surfaces. Evaluation of initial cell adhesion established a correlation between input cell number and the shifts in frequency (f) and dissipation (D). The f-shift was found to be much larger in serum-free medium as compared to a medium including serum; hence, initial cell adhesion was subsequently evaluated in serum-free medium. During the first hour of adhesion, we found a positive correlation between the QCM-D f-shift and the average area of the spread cells, as measured by cryo-scanning electron microscopy (cryo-SEM). Finally, the QCM-D technique was used to study cell adhesion on different metal oxide surfaces. Initial cell adhesion on Ta was found to induce a larger f-shift as compared to Cr, indicating larger spreading of cells on Ta. Cryo-SEM data confirmed that spreading of cells on Cr was on average only two-thirds the spreading on Ta. Our results demonstrate that the QCM-D technique is a versatile technique to quickly distinguish initial cell-surface interactions on different biomaterials.
Modin
Charlotte
C
Interdisciplinary Nanoscience Center (iNANO), University of Aarhus, DK-8000 Aarhus C, Denmark.
Stranne
Anne-Louise
AL
Foss
Morten
M
Duch
Mogens
M
Justesen
Jeannette
J
Chevallier
Jacques
J
Andersen
Lars K
LK
Hemmersam
Anne G
AG
Pedersen
Finn S
FS
Besenbacher
Flemming
F
D000818:Animals; D001672:Biocompatible Materials; D015374:Biosensing Techniques; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D002857:Chromium; D020285:Cryoelectron Microscopy; D003460:Crystallization; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D011791:Quartz; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D013635:Tantalum
D001672:Biocompatible Materials; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D002857:Chromium; D011791:Quartz; D013635:Tantalum