Affinity identification of delta-opioid receptors using latex nanoparticles.
Three types of latex nanoparticles carrying naltrindole (NTI) derivatives were synthesized as probes for the affinity isolation of their binding proteins including the delta-opioid receptor. The effect of the attachment of NTI to different positions on the linker was investigated. Only latex nanoparticles in which the NTI derivative was linked through the phenol group were useful for isolating the recombinant delta-opioid receptor solubilized from CHO cell membrane. These latex nanoparticles could be a useful tool for investigations of the pharmacological activity of NTI.
Hasegawa
Makoto
M
Frontier Collaborative Research Center and Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuda, Yokohama 226-8501, Japan.
Ohno
Hiroshi
H
Tanaka
Hiroshi
H
Hatakeyama
Mamoru
M
Kawaguchi
Haruma
H
Takahashi
Takashi
T
Handa
Hiroshi
H
D000818:Animals; D016466:CHO Cells; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D006224:Cricetinae; D003600:Cytosol / Q000378:metabolism; D004745:Enkephalins / Q000737:chemistry; D007840:Latex / Q000737:chemistry*; D008956:Models, Chemical; D009271:Naltrexone / Q000031:analogs & derivatives* / Q000737:chemistry; D049329:Nanostructures; D011485:Protein Binding; D011957:Receptors, Opioid / Q000737:chemistry; D017465:Receptors, Opioid, delta / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry
D004745:Enkephalins; D007840:Latex; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D011994:Recombinant Proteins; D009271:Naltrexone; C055382:naltrindole