Breeding of brewer's yeast by hybridization between a top-fermenting yeast Saccharomyces cerevisiae and a cryophilic yeast Saccharomyces bayanus.
To improve the fermentability of a top-fermenting yeast at low-temperature, we performed hybridization trials between four top-fermenting Saccharomyces cerevisiae strains and a cryophilic yeast Saccharomyces bayanus YM84 with good fermentability at low-temperature. The hybrids selected using 5-bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside were checked with pulsed-field gel electrophoresis and their brewing performance at the low-temperature of 10.5 degrees C was observed using small-scale (2 l) fermentation trials.
Sato
Masahide
M
Brewing Research Laboratories, Sapporo Breweries Ltd., 10-Okatohme, Yaizu, Shizuoka 425-0013, Japan. Masahide.Sato@sapporobeer.co.jp
Kishimoto
Munekazu
M
Watari
Junji
J
Takashio
Masachika
M