Characterization of acetic acid bacteria in "traditional balsamic vinegar".
This study evaluated the glucose tolerance of acetic acid bacteria strains isolated from Traditional Balsamic Vinegar. The results showed that the greatest hurdle to acetic acid bacteria growth is the high sugar concentration, since the majority of the isolated strains are inhibited by 25% of glucose. Sugar tolerance is an important technological trait because Traditional Balsamic Vinegar is made with concentrated cooked must. On the contrary, ethanol concentration of the cooked and fermented must is less significant for acetic acid bacteria growth. A tentative identification of the isolated strains was done by 16S-23S-5S rDNA PCR/RFLP technique and the isolated strains were clustered: 32 strains belong to Gluconacetobacter xylinus group, two strains to Acetobacter pasteurianus group and one to Acetobacter aceti.
Gullo
Maria
M
Department of Agricultural Science, University of Modena and Reggio Emilia, Via J. F. Kennedy, 17, 42100 Reggio Emilia, Italy. gullo.maria@unimore.it
Caggia
Cinzia
C
De Vero
Luciana
L
Giudici
Paolo
P
D019342:Acetic Acid / Q000378:metabolism*; D000091:Acetobacter / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D004275:DNA, Ribosomal / Q000032:analysis*; D005285:Fermentation; D005516:Food Microbiology; D020576:Gluconobacter / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S; D005947:Glucose; D019342:Acetic Acid