[The role of blood plasma porphyrins in the effects of He-Ne-laser on human leukocytes].
The He-Ne-laser induced effects in human blood leukocytes in the presence of autologic plasma were investigated. Experiments were performed in two ways: (1) He-Ne-laser irradiation of cells in the presence of autologic plasma or (2) laser irradiation and subsequent addition of autologic plasma to the cell suspension. The concentration dependencies of plasma additions were evaluated. To obtain different concentrations of porphyrins in plasma samples, we either diluted the samples with PBS or selected patients with different porphyrin plasma content. The effects of He-Ne-laser irradiation were characterized by the maximum effect dose (Dmax) of irradiation and the degree of maximum cell activation (Amax, priming index). In the first series of experiments, we irradiated leukocytes in the presence of autologic plasma taken from patients with pneumonia and bronchial asthma. It was found that Dmax decreased with increasing porphyrin concentration in plasma. It was observed that, at low porphyrin concentrations, Amax increased severalfold with increasing photosensitizer concentration. At a porphyrin concentration of 0.46 pmol a decrease in Amax was detected as the porhyrin concentration increased. The same effects were revealed at high doses of laser irradiation. Very similar effects were found in experiments with the addition of irradiated plasma to cells. However, the Amax value was considerably less compared to that after irradiation in the presence of plasma (160% vs. 230 - 270% upon combined irradiation of cells and plasma). The Dmax value was higher in the series of experiments in which plasma was irradiated separately. The results suggest that laser-induced leukocyte activation can be mediated by blood plasma porphyrins and the products of lipid peroxidation formed as a result of porphyrin-photosensitized lipid oxidation.
Klebanov
G I
GI
Chichuk
T V
TV
Osipov
A N
AN
Vladimirov
Iu A
IuA
D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007834:Lasers; D007962:Leukocytes / Q000502:physiology / Q000528:radiation effects*; D015227:Lipid Peroxidation / Q000528:radiation effects*; D010084:Oxidation-Reduction / Q000528:radiation effects; D010949:Plasma / Q000737:chemistry / Q000378:metabolism*; D011166:Porphyrins / Q000097:blood* / Q000737:chemistry
D011166:Porphyrins