Screening for and purification of novel self-aggregatable lectins reveal a new functional lectin group in the bark of leguminous trees.
A solubility-insolubility transition assay was used to screen the bark and stems of seven leguminous trees and plants for self-aggregatable lectins. Novel lectins were found in two trees, Robinia pseudoacacia and Wisteria floribunda, but not in the leguminous plants. The Robinia lectin was isolated from coexisting lectin by combined affinity chromatographies on various sugar adsorbents. The purified lectins proved to be differently glycosylated glycoproteins. One lectin exhibited the remarkable characteristics of self-aggregatable lectins: localization in the bark of legume trees, self-aggregation dissociated by N-acetylglucosamine/mannose, and coexistence with N-acetylgalactosamine/galactose-specific lectins, which are potential endogenous receptors. Self-aggregatable lectins are a functional lectin group that can link enhanced photosynthesis to dissociation of glycoproteins.
Ina
Chieko
C
Department of Advanced Biosciences, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan.
Sano
Kotone
K
Yamamoto-Takahashi
Makiko
M
Matsushita-Oikawa
Hiroko
H
Takekawa
Hiroko
H
Takehara
Yayoi
Y
Ueda
Haruko
H
Ogawa
Haruko
H
D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007887:Fabaceae / Q000737:chemistry*; D007564:Japan; D009005:Monosaccharides / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry*; D037121:Plant Lectins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*; D011485:Protein Binding; D012995:Solubility
D009005:Monosaccharides; D037121:Plant Lectins