title: An instant measurement of oxidoreductase activity above 100 degrees C by monitoring the absorbance change.
abstract: A conventional absorbance monitoring method using a cuvette covered with a tight rubber cap was found to be applicable for measuring oxidoreductase activity at temperatures up to 115 degrees C. Using this method, the optimal temperatures of the enzymes, including oxygen-sensitive enzymes from a hyperthermophilic archaeon Thermococcus profundus, were determined.
authors.affiliation: Department of Life Science, Graduate School of Life Science, Rikkyo (St. Paul's) University, 3-34-1 Nishi-Ikebukuro, Toshima-ku, Tokyo 171-8501, Japan. imaitak@rikkyo.ac.jp
authors.forename: Takeo
authors.initials: T
authors.lastname: Imai
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yasujima
authors.forename: Masood Ahmed
authors.initials: MA
authors.lastname: Siddiqui