title: Physicochemical interactions at the dentin/adhesive interface using FTIR chemical imaging.
abstract: To date, much of our understanding of dentin bonding has been based on investigations performed on sound, healthy dentin. This is not the substrate generally encountered in clinical practice, rather dentists must frequently bond to caries-affected dentin. Because of the extreme complexity and variability of the caries-affected dentin substrate, conventional characterization techniques do not provide adequate information for defining those factors that impact bond formation. Using Fourier-transform infrared imaging, we characterized the inhomogeneities and compositional differences across the length and breadth of the caries-affected dentin/adhesive interface. Differences in mineral/matrix ratio, crystallinity, and collagen organization were noted in the comparison of caries-affected and healthy dentin. As compared to healthy dentin, there were striking differences in depth of demineralization, adhesive infiltration, and degree of conversion at the interface with caries-affected dentin.
authors.affiliation: University of Missouri-Kansas City, School of Dentistry, Department of Pediatric Dentistry, Kansas City, Missouri 64108, USA. spencerp@umkc.edu
authors.forename: Paulette
authors.initials: P
authors.lastname: Spencer
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: J Lawrence
authors.initials: JL
authors.lastname: Katz
authors.forename: Anil
authors.initials: A
authors.lastname: Misra
mesh_terms: D000268:Adhesiveness; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry* / Q000627:therapeutic use; D003731:Dental Caries / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D003804:Dentin / Q000737:chemistry* / Q000473:pathology* / Q000503:physiopathology; D017220:Dentin-Bonding Agents / Q000737:chemistry* / Q000627:therapeutic use; D003952:Diagnostic Imaging / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing / Q000379:methods; D008963:Molar / Q000737:chemistry / Q000473:pathology / Q000503:physiopathology; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013499:Surface Properties
chemical_list: D017220:Dentin-Bonding Agents; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate