Detection of the myo-inositol 4.06-ppm resonance by selective J rewinding: application to human prefrontal cortex in vivo.
A new proton NMR single-voxel spectral editing strategy for the rapid measurement of myo-inositol in human brain is proposed. The spectral editing detects the 4.06-ppm, weakly coupled resonance by means of selective J rewinding. An 84.6-ms-long quadruple-resonance selective 180 degrees radiofrequency pulse, implemented within an adiabatic-refocused localization sequence, induces an in-phase triplet at 4.06 ppm, while eliminating the contribution from creatine, phosphorylethanolamine, lactate, and serine in this spectral region. The myo-inositol concentration in human prefrontal cortex is estimated to be 5.7 +/- 0.9 mumol/g (mean +/- SD, n = 7), with reference to NAA at 10 micromol/g.
Choi
Changho
C
Department of Biomedical Engineering, University of Alberta, Edmonton, Alberta, Canada. changho.choi@ualberta.ca
Ogilvie
Catherine J
CJ
Malykhin
Nikolai
N
Ngo
Jennifer T V
JT
Hartfeil
Misha A W
MA
Coupland
Nicholas J
NJ
D000465:Algorithms; D001921:Brain / Q000378:metabolism*; D006801:Humans; D007294:Inositol / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008832:Microchemistry / Q000379:methods*; D017397:Prefrontal Cortex / Q000378:metabolism*
D007294:Inositol