[The effect of CSN1 S2, CSN3 and beta-lg genes on milk performance in Xinong Saanen dairy goat].
PCR-RFLP technique was applied to analyze correlation between the polymorphisms of CSN1 S2 (alpha(s2) casein), CSN3 (kappa casein) and beta-lg (beta-lactoglobulin) genes and milk performance in 69 individuals of Xinong Saanen dairy goat. The results showed that there was significant correlation between different genotypes of CSN1 S2 locus and milk yield:average milk yield of individuals with genotype FF was less than that of genotype NN (P < 0.05); the second lactation milk yield of individuals with genotype NN was over 90 kg higher than that with genotype FF (P < 0.01). This indicates that allele F of CSN1 S2 locus probably has significant negative effect on milk yield. The results of CSN3 gene digested with endonuclease Hind III cleavage showed that no significant difference of milk yield between genotype DE and genotype EE was detected in first, second, third and fourth lactation milk yield and average milk yield (P > 0.05). The results of CSN3 gene with endonuclease Taq I cleavage showed that no significant difference of milk yield among individuals with genotype TT, TC and CC was detected (P > 0.05). No polymorphism was detected in PCR products of CSN3 gene digested with endonuclease Hae III. The analysis of beta-lg gene's 5' flanking region (710 bp) by PCR-RFLP in Xinong Saanen dairy goat showed that milk yield of individuals with genotype AA was higher than that with genotype AB in second, third lactation milk yield and average milk yield (P < 0.05). The results implied that allele A of beta-lg gene's 5' flanking region is probably related to high milk yield.
Chen
Hong
H
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Agricultural Molecular Biology, Yangling 712100, China. chenghong1212@263.net
Lan
Xian-Yong
XY
Li
Rui-Biao
RB
Lei
Chu-Zhao
CZ
Sun
Wei-Bin
WB
Zhang
Run-Feng
RF
Zheng
Yuan-Lin
YL
Zhu
Bi-Cai
BC
D024506:5' Flanking Region; D000483:Alleles; D000818:Animals; D002364:Caseins / Q000235:genetics*; D004247:DNA / Q000032:analysis / Q000235:genetics; D004586:Electrophoresis / Q000379:methods; D005260:Female; D005838:Genotype; D006041:Goats / Q000235:genetics*; D007774:Lactation; D007782:Lactoglobulins / Q000235:genetics*; D008892:Milk / Q000737:chemistry* / Q000592:standards; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D002364:Caseins; D007782:Lactoglobulins; D004247:DNA