Liver X receptors mediate inhibition of hCG secretion in a human placental trophoblast cell line.
Liver X receptors (LXR) alpha and beta are important regulators of lipid homeostasis in liver, adipose and other tissues. However, no such information is available for the human placenta. We determined expression of both LXR alpha and beta in placental trophoblast cell lines, BeWo and JAR. Exposure of BeWo cells to a synthetic LXR agonist, T0901317, resulted in an increase in the amount of mRNA of LXR target genes, sterol regulatory element-binding protein-1 and fatty acid synthase. T0901317 also increased the synthesis of lipids. Moreover, T0901317 resulted in a reduced secretion of hCG during differentiation of these cells. Our data for the first time demonstrate a new role for LXRs in the human placenta.
Weedon-Fekjaer
M S
MS
Department of Nutrition, University of Oslo, POB 1046 Blindern, N-0316 Oslo, Norway.
Duttaroy
A K
AK
Nebb
H I
HI
D015152:Blotting, Northern; D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D006063:Chorionic Gonadotropin / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000819:agonists / Q000096:biosynthesis / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D005260:Female; D006801:Humans; D006845:Hydrocarbons, Fluorinated; D006888:Hydroxycholesterols / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D011247:Pregnancy; D012313:RNA / Q000737:chemistry / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000819:agonists / Q000096:biosynthesis / Q000378:metabolism*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology; D014162:Transfection; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism*; D005466:Fluorocarbons; D000096926:Benzenesulfonamides
D006063:Chorionic Gonadotropin; D004268:DNA-Binding Proteins; D064429:Fatty Acid Synthases; D006845:Hydrocarbons, Fluorinated; D006888:Hydroxycholesterols; D008055:Lipids; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D012313:RNA; D018160:Receptors, Cytoplasmic and Nuclear; D051780:Sterol Regulatory Element Binding Protein 1; D013449:Sulfonamides; C000605213:NR1H3 protein, human; C497387:SREBF1 protein, human; C423915:T0901317; D005466:Fluorocarbons; D000096926:Benzenesulfonamides; C003585:22-hydroxycholesterol