title: Nutritional and hypoglycemic effect of fruit pulp of Annona squamosa in normal healthy and alloxan-induced diabetic rabbits.
abstract: The nutritive value of the pulp of the edible fruit of Annona squamosa and its effect on various biochemical parameters has been assessed in normal and alloxan-induced diabetic rats. Different doses (2.5, 5.0, 10.0 g/kg b.w.) of fresh fruit pulp of A. squamosa were given to the three groups each of normal healthy and diabetic rabbits orally daily for 1 month. There was a control group of normal as well as diabetic animals which did not receive fruit pulp. Protein efficiency ratio (PER), digestibility coefficient (DC), biological value (BV) and net protein utilization (NPU) were calculated and improvement in the nutritional level was studied by comparing with the control group. Effect of the fruit pulp was also studied on various biochemical parameters, namely fasting blood glucose (FBG), total cholesterol (TCH), HDL-cholesterol, triglyceride (TG), total protein (TPR), alkaline phosphatase (ALKP), serum glutamate oxaloacetate and pyruvate transaminases (SGOT and SGPT), serum creatinine (CRTN) and serum bilirubin (BIL). Protein and glucose in urine were also estimated. Total hemoglobin and glycohemoglobin (HbAc) were estimated in blood before and after 1 month of feeding fruit pulp. Fruit pulp increased the net protein utilization by 29.3 in normal healthy rabbits with 10 g/kg b.w. and 34.1 in induced diabetic (induced by alloxan) animals with 5 g/kg b.w. of the fruit pulp feeding when compared with the control group of rabbits (p < 0.001). Feeding fruit pulp with the same amount increased the total hemoglobin content by 21.0% in normal rabbits and 10.8% in diabetic rabbits. Fruit pulp also reduced the total cholesterol level by 45-46% in normal and 32.4% in diabetic animals with increased HDL-cholesterol. Feeding pulp improved the liver function in normal as well as diabetic rabbit as shown by reduction in the serum SGOT, SGPT, ALKP and bilirubin levels. The optimal improvement in nutritive value of normal animals was found with 5.0-10.0 g/kg b.w. of the fruit pulp feeding, while in diabetic animals it was 2.5-5.0 g/kg b.w. In the diabetic animals pulp feeding between 2.5 and 5.0 g/kg b.w. showed improvement in the glucose tolerance. Further, 5 g/kg b.w. of fruit pulp brought down urine sugar, urine protein and glycohemoglobin in diabetic rabbits. Feeding pulp had increased utilization of dietary protein, body weight as well as the ratio of gain in body weight per gram of protein consumed. It had a protective effect on liver and heart as indicated by reduction in the SGOT, SGPT, ALKP and serum bilirubin levels.
authors.affiliation: Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, India.
authors.forename: Rajesh Kumar
authors.initials: RK
authors.lastname: Gupta
authors.forename: Achyut Narayan
authors.initials: AN
authors.lastname: Kesari
authors.forename: Geeta
authors.initials: G
authors.lastname: Watal
authors.forename: P S
authors.initials: PS
authors.lastname: Murthy
authors.forename: Ramesh
authors.initials: R
authors.lastname: Chandra
authors.forename: Vibha
authors.initials: V
authors.lastname: Tandon
mesh_terms: D000284:Administration, Oral; D000818:Animals; D031041:Annona / Q000737:chemistry*; D001663:Bilirubin / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004063:Digestion; D004305:Dose-Response Relationship, Drug; D005638:Fruit; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D009753:Nutritive Value; D008517:Phytotherapy / Q000662:veterinary*; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use*; D011817:Rabbits; D011897:Random Allocation; D015430:Weight Gain / Q000187:drug effects / Q000502:physiology
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D008055:Lipids; D010936:Plant Extracts; D001663:Bilirubin