title: [Detection of p53 gene mutation in the plasma of gastric cancer patients].
abstract: OBJECTIVE
  To investigated p53 gene mutation in plasma of gastric cancer patients.
  
  
  METHODS
  DNA extracted from plasma and matched tumor and tumor-adjacent normal tissues of 96 gastric cancer patients, and DNA from 20 healthy people were studied. Exons 5, 6, 7, and 8 of p53 were amplified by PCR. The mutation status was analyzed by denaturing high-performance liquid chromatography (DHPLC), followed by direct sequencing of cases with aberrant chromatographic patterns.
  
  
  RESULTS
  Heterozygous mutations of p53 gene were detected in 19.9% (19/96) of primary tumor tissues and 5.2% (5/96) of corresponding plasma. All p53 gene mutations detected in plasma DNA consisted with mutations in the matched primary tumor samples. Neither the tumor-adjacent gastric mucosa tissues nor control plasma from healthy volunteers showed p53 gene mutation. No correlation was found between p53 mutation status and clinicopathological features of gastric cancer patients.
  
  
  CONCLUSIONS
  p53 gene mutation in plasma can be detected in tissues and plasma of gastric cancer patients, which could be applied in screening and surveillance of this disease.
authors.affiliation: Peking University School of Oncology, Beijing Institute for Cancer Research, Beijing Cancer Hospital, Beijing 100036, China.
authors.forename: Peng-cheng
authors.initials: PC
authors.lastname: Su
authors.forename: Lian-hai
authors.initials: LH
authors.lastname: Zhang
authors.forename: Wen-hui
authors.initials: WH
authors.lastname: Wan
authors.forename: Hui
authors.initials: H
authors.lastname: Ren
authors.forename: Gui-guo
authors.initials: GG
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Guo-ren
authors.initials: GR
authors.lastname: Deng
authors.forename: Jia-fu
authors.initials: JF
authors.lastname: Ji
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000097:blood / Q000737:chemistry / Q000235:genetics; D005091:Exons; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53