title: Effect of polymorphism of uncoupling protein 3 gene -55 (C>T) on the resting energy expenditure, total body fat and regional body fat in Chinese.
abstract: OBJECTIVE
  To investigate the relationship of the C to T variant at the -55 site of the promoter region of uncoupling protein 3 gene (UCP3) with the resting energy expenditure and the parameters of body fat in Chinese population.
  
  
  METHODS
  Three hundred Chinese (91 normal weight subjects, 209 overweight/obesity subjects) were genotyped for the UCP3 gene -55(C>T) by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Resting energy expenditure (REE), fat mass (FM), fat free mass (FFM) and the parameters for regional adipose tissue distribution were measured.
  
  
  RESULTS
  Genotype frequencies of UCP3 gene -55(C>T) were not associated with obesity and different types of obesity. The REE level in normal weight subjects with TT homozygotes was higher than that in those with CT heterozygotes and CC homozygotes (P=0.0200). Similar tendency was also observed in overweight/obesity subjects. The FM/FFM exhibited significant difference between the overweight/obesity subjects with a TT genotype and those with a CT or CC genotype (P=0.0096).
  
  
  CONCLUSIONS
  The level of difference in REE caused by the polymorphism of promoter region of UCP3 -55(C>T) may play a role in energy metabolism in Chinese.
authors.affiliation: Shanghai Diabetes Institute, Department of Endocrinology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, PR China. fqichen@yahoo.com.cn
authors.forename: Qi-chen
authors.initials: QC
authors.lastname: Fang
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: Jia
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Yu-qian
authors.initials: YQ
authors.lastname: Bao
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Kun-san
authors.initials: KS
authors.lastname: Xiang
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D044466:Asian People / Q000235:genetics; D002681:China; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000235:genetics* / Q000502:physiology; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D000071247:Uncoupling Protein 3
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604392:UCP3 protein, human; D000071247:Uncoupling Protein 3