Electrotransformation of Clostridium paraputrificum M-21 with some plasmids.
Clostridium paraputrificum M-21 was transformed with several shuttle plasmids constructed for Clostridium acetobutylicum-Escherichia coli and Clostridium perfringens-E. coli by electroporation. The Clostridium stercorarium xylanase gene xyn10B was successfully expressed in C. paraputrificum M-21 and the expressed protein did not suffer from proteolysis by host protease(s). This system will provide us with a genetic tool for genetic and metabolic engineering of this bacterium.
Sakka
Kazuo
K
Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan. sakka@bio.mie-u.ac.jp
Kawase
Mamiko
M
Baba
Daisuke
D
Morimoto
Kenji
K
Karita
Shuichi
S
Kimura
Tetsuya
T
Ohmiya
Kunio
K