The effect of PAMAM dendrimers on human and bovine serum albumin at different pH and NaCl concentrations.
The effect of PAMAM G3.5, PAMAM G4 and PAMAM-OH G4 dendrimers on human and bovine serum albumins has been studied by fluorescence spectroscopy at different pH and ionic strength. It has been shown that the interactions between dendrimers and proteins depend on pH and the efficiency of interactions can be regulated by changing pH. The maximal pH dependence was observed for interactions between albumins and PAMAM G4 dendrimer. At physiological pH all dendrimers affect proteins in the maximum degree. Dendrimers had no effect on N-F and N-B transitions of albumins. The effect of dendrimers on HSA was smaller than for BSA. The increase of NaCl concentration led to a decrease of interactions between dendrimers and proteins.
Shcharbin
D
D
Institute of Biophysics and Cellular Engineering, National Academy of Sciences of Belarus, Minsk, Belarus.
Klajnert
B
B
Bryszewska
M
M
D000818:Animals; D002417:Cattle; D050091:Dendrimers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D011073:Polyamines / Q000737:chemistry / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D012709:Serum Albumin / Q000737:chemistry / Q000187:drug effects*; D012965:Sodium Chloride; D013050:Spectrometry, Fluorescence
D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines; D012709:Serum Albumin; D012965:Sodium Chloride