Coupling of MBP fusion protein cleavage with sparse matrix crystallization screens to overcome problematic protein solubility.
Gruswitz
Franz
F
University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.
Frishman
Mary
M
Goldstein
Barry M
BM
Wedekind
Joseph E
JE
D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D002352:Carrier Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007168:IMP Dehydrogenase / Q000737:chemistry; D058619:Maltose-Binding Proteins; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012984:Software; D012995:Solubility
D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D011993:Recombinant Fusion Proteins; D007168:IMP Dehydrogenase; C501687:IMPDH2 protein, human