title: A novel neutralizing monoclonal antibody against cell-binding polypeptide of ricin.
abstract: One strain of neutralizing monoclonal antibody (MAb) against cell-binding polypeptide of ricin, named 3E1, was generated efficiently. The antibody recognized the linearity epitope of RTB located in a toxin structure domain characterized by Western blotting. The safe period of mice for intraperitoneal injection of 100 microg of antibody was 20 min after intraperitoneal injection of 2 microg of Ricin (10 times LD50). The neutralizing MAb we obtained could be developed into an immunotherapeutic agent to counteract the use of ricin as a terrorist or biological warfare weapon. It might be useful, as well, for antibody-based prophylaxis.
authors.affiliation: Department of Molecular Immunology, Institute of Basic Medical Sciences, Beijing, P.R. China., Clinical Laboratory, JinCheng Hospital, Lan Zhou, P.R. China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Guo
authors.forename: Bei-Fen
authors.initials: BF
authors.lastname: Shen
authors.forename: Jian-Nan
authors.initials: JN
authors.lastname: Feng
authors.forename: Ying-Xun
authors.initials: YX
authors.lastname: Sun
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Mei-Ru
authors.initials: MR
authors.lastname: Hu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D020128:Inhibitory Concentration 50; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests / Q000379:methods; D010455:Peptides / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000633:toxicity; D012276:Ricin / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000633:toxicity; D023382:Toxicity Tests, Acute
chemical_list: D000911:Antibodies, Monoclonal; D010455:Peptides; D012276:Ricin