Glycine-rich antifreeze proteins from snow fleas.
We purified antifreeze proteins from winter-active snow fleas, Hypogastrura harveyi. These 6.5- and 15.7-kilodalton thermolabile proteins are glycine-rich (45% of the residues), and the short isoform is composed of the tripeptide repeat Gly-X-X. This makes them very different from other antifreeze proteins, including two from insects, suggesting independent adaptation to freezing environments.
Graham
Laurie A
LA
Department of Biochemistry, Queen's University, Kingston, ON K7L 3N6, Canada.
Davies
Peter L
PL
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D021301:Antifreeze Proteins / Q000737:chemistry* / Q000302:isolation & purification; D001181:Arthropods / Q000737:chemistry*; D002942:Circular Dichroism; D019143:Evolution, Molecular; D005998:Glycine / Q000032:analysis*; D007053:Ice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012914:Snow
D000596:Amino Acids; D021301:Antifreeze Proteins; D007053:Ice; D005998:Glycine