title: Immobilization of Arg-Gly-Asp (RGD) sequence in sugar containing copolymer for culturing of pheochromocytoma (PC12) cells.
abstract: A copolymer that included a Gly-Arg-Gly-Asp-Ser (GRGDS) sequence and sugar moieties was synthesized to culture pheochromocytoma cells (PC12). PC12 cells attached to poly(N-p-vinylbenzyl-D-maltonamide-co-6-(p-vinylbenzamido)-hexanoic acid-g-GRGDS) [p(VMA-co-VBGRGDS)]-coated dishes showed greater proliferation than on other polymer-coated surfaces. Enhancement in cell growth correlated with the ability of soluble Arg-Gly-Asp (RGD) to inhibit cell adhesion to surfaces coated with p(VMA-co-VBGRGDS). This method for promoting cell proliferation may be useful for the culturing of anchorage-dependent cells. Furthermore, about 80% greater dopamine secretion from PC12 cells was produced with the p(VMA-co-VBGRGDS) as compared with PC12 cells on unmodified surfaces.
authors.affiliation: School of Medical Engineering, Ajou University, Suwon City 442-749, Korea.
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
authors.forename: Kun
authors.initials: K
authors.lastname: Na
authors.forename: Kang Choon
authors.initials: KC
authors.lastname: Lee