title: Microcyn: a novel super-oxidized water with neutral pH and disinfectant activity.
abstract: A new super-oxidized water (SOW) product, Microcyn, was tested for in vitro antimicrobial and antiviral activities. The effectiveness of this neutral-pH SOW at killing Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Candida albicans in pure culture was evaluated. One millilitre (approximately 10(8)colony-forming units/mL) of each micro-organism was subjected to 9 mL Microcyn or sterile water at room temperature for 30s. Under these conditions, a log(10) reduction factor of 8 in the level of all pathogens occurred in the treatment samples. In addition, results of tests with three batches of Microcyn exposed to Bacillus atrophaeus spores for 5 min demonstrated complete inactivation of the spores within 2-3 min (log(10) reduction factor >4). The effectiveness of Microcyn in reducing human immunodeficiency virus-1 (HIV-1) on hard surfaces (glass) was also evaluated in compliance with Environmental Protection Agency requirements for virucidal claims. After exposure of the tested surfaces to Microcyn for 5 min without agitation, there was a log(10) reduction factor >3 in the viral load as measured by both cytopathic effect and antigen p24 of HIV-1 production in MT-2 cultures. Microcyn activity against adenoviral vector type 5 was also analysed under simulated laboratory in-use conditions with viral suspensions. In order to increase the sensitivity of the test, the fluorescent light emitted by AdGFP-infected cells was measured with the use of a flow cytometer. A log(10) reduction factor >3 in the viral load was achieved after a 5-min exposure to Microcyn under these strict conditions. These results show that Microcyn exerts a wide antimicrobial spectrum with major advantages over acidic SOWs, including neutral pH, lower free active chlorine (51-85 ppm) and long shelf life (1 year).
authors.affiliation: Instituto Nacional de Rehabilitación, Secretaría de Salud, Mexico, DF.
authors.forename: C
authors.initials: C
authors.lastname: Landa-Solis
authors.forename: D
authors.initials: D
authors.lastname: González-Espinosa
authors.forename: B
authors.initials: B
authors.lastname: Guzmán-Soriano
authors.forename: M
authors.initials: M
authors.lastname: Snyder
authors.forename: G
authors.initials: G
authors.lastname: Reyes-Terán
authors.forename: K
authors.initials: K
authors.lastname: Torres
authors.forename: A A
authors.initials: AA
authors.lastname: Gutierrez
mesh_terms: D000256:Adenoviridae / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D002460:Cell Line; D015169:Colony Count, Microbial; D003588:Cytopathogenic Effect, Viral; D004202:Disinfectants / Q000494:pharmacology*; D005434:Flow Cytometry; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D013171:Spores, Bacterial / Q000187:drug effects; D019562:Viral Load
chemical_list: D004202:Disinfectants; D016655:HIV Core Protein p24; C118085:superoxidized water; D049452:Green Fluorescent Proteins; D006861:Hydrogen Peroxide