Improved immobilization yields by addition of protecting agents in glutaraldehyde-induced immobilization of protease.
The effects of peptides and polyethylene glycol (PEG) on the immobilization of protease using glutaraldehyde-activated Lewatit R258-K resin were investigated. When soy peptide was added at 0.1 g/l, the activity recovery and effectiveness factor increased by 20% and 38%, respectively. When PEG4000 (0.5 g/l) was used as a protecting agent, the activity recovery and effectiveness factor were improved by 35% and 60%, respectively, leading to an increased specific activity of the immobilized enzyme. The addition of PEG4000 is suggested to improve the cost-effectiveness for the production of immobilized enzymes.
Chae
H J
HJ
Department of Food Technology, Hoseo University, Asan 336-795, Korea.
Kim
E Y
EY
In
M
M