A facile transformation of Arabidopsis thaliana using ceramic supported propagation system.
Arabidopsis thaliana can be a useful model plant in the field of plant molecular biology because it is a sole dicotyledonous plant whose genome sequence has been completely determined at the present time. To develop functional genomics of A. thaliana, a large number of transgenic plants must be produced. However, transformation of the plants requires wide space and long time. For these reasons, a highly efficient method has been desired to be developed. In order to save space, the transformation system was improved, based on a new technology for plant propagation using ceramic tubes. The new method using ceramic tubes facilitated to prepare plants with a uniform growth level. In addition, the colander procedure after infiltration, which is one of tedious procedures, could be omitted. The new system without soil usage could cut down the cleaning up after experiment.
Fukusaki
Ei-Ichiro
E
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Ikeda
Tatsuhiko
T
Suzumura
Daisuke
D
Kobayashi
Akio
A