Identification and characterization of a triacylglycerol lipase in Arabidopsis homologous to mammalian acid lipases.
Triacylglycerol (TAG) lipases have been thoroughly characterized in mammals and microorganisms. By contrast, very little is known on plant TAG lipases. An Arabidopsis cDNA called AtLip1 (At2g15230), which exhibits strong homology to lysosomal acid lipase, was found to drive the synthesis of an active TAG lipase when expressed in the baculovirus system. The lipase had a maximal activity at pH 6 and the specific activity was estimated to be about 45 micromol min(-1) mg(-1) protein using triolein as a substrate. Knock-out mutant analysis showed no phenotype during germination indicating that this enzyme is fully dispensable for TAG storage breakdown during germination. Northern blot analyses indicated that the transcript is present in all tissues tested.
El-Kouhen
Karim
K
Laboratoire d'Enzymologie Interfaciale et de Physiologie de la Lipolyse (EIPL), CNRS UPR 9025, Université de la Méditerranée Aix-Marseille II, Inst. Biologie Struct. and Micro., 31 Chemin Joseph Aiguier, 13402 Marseille cedex 20, France.
Blangy
Stéphanie
S
Ortiz
Emilia
E
Gardies
Anne-Marie
AM
Ferté
Natalie
N
Arondel
Vincent
V
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016367:Baculoviridae / Q000235:genetics; D015870:Gene Expression; D018525:Germination; D006801:Humans; D008049:Lipase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation; D014280:Triglycerides / Q000378:metabolism
D029681:Arabidopsis Proteins; D014280:Triglycerides; D008049:Lipase