Development of gene delivery systems for the epsilon-poly-L-lysine producer, Streptomyces albulus.
Streptomyces albulus IFO14147 produces epsilon-poly-L-lysine (epsilon-PL), an amino acid homopolymer antibiotic that is used as a food preservative in many countries, including Japan, South Korea, and the United States. To construct an overproducer of the industrially important epsilon-PL and to develop a deeper understanding of its biosynthetic mechanism, we developed systems for DNA delivery into the S. albulus strain based on both polyethylene glycol-mediated protoplast transformation and intergeneric conjugation from Escherichia coli. The successes of these transformations in particular were accomplished by employing the new cryptic-plasmid-based shuttle vectors constructed in this study. The genetic systems developed here should facilitate a molecular genetic approach to S. albulus.
Hamano
Yoshimitsu
Y
Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka-cho, Fukui 910-1195, Japan. hamano@fpu.ac.jp
Nicchu
Ine
I
Hoshino
Yusuke
Y
Kawai
Takahiro
T
Nakamori
Shigeru
S
Takagi
Hiroshi
H
D016503:Drug Delivery Systems / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000096:biosynthesis*; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D014169:Transformation, Bacterial
D011107:Polylysine; D011092:Polyethylene Glycols