title: Effects of administration of beta-carotene, ascorbic acid, persimmons, and pods on antioxidative ability in UV-irradiated ODS rats.
abstract: To evaluate the effects of supplementing diets with carotenoid and ascorbic acid (AsA) on the antioxidative ability of Osteogenic Disorder-Shionogi (ODS) rats, we added synthetic beta-carotene (betaC), AsA, and powders of persimmon (Ka) and pods (Po) containing betaC and AsA to the diet and obtained the following results. The urinary 8-hydroxydeoxyguanosine (8-OHdG) concentration was low in the -betaC.AsA and +AsA groups but high in the +betaC.AsA, +Ka, and +Po groups. The thiobarbituric acid-reactive substances (TBARS) in both the liver and skin were higher in the -betaC.AsA group than in the +betaC.AsA group and were low in the +Ka and +Po groups. As antioxidant enzymes, glutathione peroxidase (GSH-Px) activity was high in the +betaC.AsA group, low in the -beta3C.AsA group in both the skin and liver, and also high in the + Ka and +Po group in the liver. Superoxide dismutase (SOD) activity was high in the -betaC.AsA group and low in the +betaC.AsA and +Ka groups in both the skin and liver. Catalase (CAT) activity in the liver was low in the -betaC.AsA, +AsA, and +betaC groups and high in the +betaC.AsA and +Po groups. These results confirmed that the administration of betaC, AsA, and persimmons and pods increases antioxidative ability in the skin and liver of ultraviolet-b(UV-B)-irradiated ODS rats.
authors.affiliation: Faculty of Education, Wakayama University, Japan. hosotani@center.wakayama-u.ac.jp
authors.forename: Keisuke
authors.initials: K
authors.lastname: Hosotani
authors.forename: Minoru
authors.initials: M
authors.lastname: Yoshida
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kitagawa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000378:metabolism; D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000378:metabolism; D019587:Dietary Supplements; D029788:Diospyros; D005638:Fruit; D008099:Liver / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects / Q000528:radiation effects; D028321:Plant Preparations / Q000008:administration & dosage*; D051381:Rats; D012867:Skin / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects; D019207:beta Carotene / Q000008:administration & dosage* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D028321:Plant Preparations; D017392:Thiobarbituric Acid Reactive Substances; D019207:beta Carotene; D001205:Ascorbic Acid