[Establishment of cellular hypoxia model and its effects on biological behavior of gastric cancer cell line SGC-7901].
OBJECTIVE
Hypoxia is an elementary characteristic of tumor microenvironment. Establishing hypoxia microenvironment in vitro will be of help in studying the effects of hypoxia on tumor cells. Previous methods of establishing hypoxic cell culture model are fussy, and researches on the effects of hypoxia on gastric cancer cells are rare. This study was to establish hypoxia microenvironment with GasPaK method in vitro, and observe the effects of hypoxia on biological features of gastric cancer cell line SGC-7901.


METHODS
The hypoxia microenvironment was established by GasPaK method; the changes of PO2, PCO2, and pH in RPMI-1640 were monitored by blood gas analysis. The effect of hypoxia on cell cycle of SGC-7901 cells was analyzed by flow cytometry (FCM). The morphology of SGC-7901 cells was observed under light microscope and transmission electron microscope. Live cells were counted with trypan blue staining. The adhesiveness of SGC-7901 cells was detected by MTT assay; migration ability of SGC-7901 cells was assessed by movement experiment.


RESULTS
GasPaK formed hypoxia microenvironment in 0.5-48 h with stable PO2, PCO2, and pH. When cultured in hypoxia for 16 h, no significant change in cell cycle of SGC-7901 cells was observed; morphology of some SGC-7901 cells was changed; the number of live cells didn't significantly decreased; the adhesiveness and migration ability of SGC-7901 cells were significantly enhanced.


CONCLUSIONS
GasPaK method can stably establish hypoxia microenvironment with good repetition. Hypoxia has slight effect on SGC-7901 cells, and may induce morphologic change of SGC-7901 cells.
Zhang
Jun-Wen
JW
Department of Gastroenterology, The First Affiliated Hospital, Chongqing University Medical Sciences, Chongqing, P. R. China. duanhong@163.com
Wang
Pi-Long
PL
Yang
Zhi-Bang
ZB
D000818:Animals; D002448:Cell Adhesion; D002453:Cell Cycle; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D013274:Stomach Neoplasms / Q000473:pathology*