Production of a thermostable archaeal superoxide reductase in plant cells.
Pyrococcus furiosus superoxide reductase (SOR) is a thermostable archaeal enzyme that reduces superoxide without producing oxygen. When produced as a fusion protein with the green fluorescent protein in plant cells, P. furiosus SOR is located in the cytosol and nucleus. The recombinant SOR enzyme retains its function and heat stability when assayed in vitro. Importantly, expressing SOR in plant cells enhances their survival at high temperature indicating that it functions in vivo. The archaeal SOR provides a novel mechanism to reduce superoxide and demonstrates the potential for using archaeal genes to alter eukaryotic metabolism.
Im
Yang Ju
YJ
Plant Biology, North Carolina State University, Raleigh, 27695, USA.
Ji
Mikyoung
M
Lee
Alice M
AM
Boss
Wendy F
WF
Grunden
Amy M
AM
D019843:Archaeal Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D003593:Cytoplasm / Q000737:chemistry; D004795:Enzyme Stability; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000096:biosynthesis; D006358:Hot Temperature; D010088:Oxidoreductases / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D020027:Pyrococcus furiosus / Q000201:enzymology; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis*; D013481:Superoxides / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000378:metabolism
D019843:Archaeal Proteins; D011993:Recombinant Fusion Proteins; D013481:Superoxides; D049452:Green Fluorescent Proteins; D010088:Oxidoreductases; C121849:superoxide reductase