title: A comparative study on Pb2+ accumulation between Saccharomyces cerevisiae and Aureobasidium pullulans by SEM (Scanning Electron Microscopy) and EDX (Energy Dispersive X-Ray) analyses.
abstract: Scanning electron microscopy and energy dispersive X-ray analyses were carried out in a comparative investigation of Pb(2+) accumulation in Saccharomyces cerevisiae and Aureobasidium pullulans. In S. cerevisiae, the time required to reach an equilibrium state was shortened from 100 h to 1 h as the initial Pb(2+) concentration decreased from 96.5 mg/l to 16.0 mg/l, whereas the time was almost independent of initial Pb(2+) concentration in A. pullulans. Concomitant with the Pb(2+) accumulation, the cell surface of S. cerevisiae became rough and the amounts of potassium, phosphorus and sulfur on the cell surface decreased. However, significant increase of Pb(2+) on the cell surface after Pb(2+) accumulation was not observed due to Pb(2+) penetration into the cell interior. In contrast, the Pb(2+) accumulation had no significant effect on the surface characteristics of A. pullulans and extreme Pb(2+) accumulation was observed on the cell surface because Pb(2+) could not penetrate into the cell interior due to the existence of extracellular polymeric substances.
authors.affiliation: Department of Chemical Technology, Ulsan College, Ulsan 680-749, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Suh
authors.forename: J W
authors.initials: JW
authors.lastname: Yun
authors.forename: D S
authors.initials: DS
authors.lastname: Kim
authors.forename: S K
authors.initials: SK
authors.lastname: Song