Minimally invasive method for murine brain fixation.
Complete brain fixation can be achieved with transthoracic cardiac infusion without thoracotomy. Light and electron microscopy tissue sections reveal preservation of cytoplasmic and nuclear structure at all magnification levels. Punched samples were obtained from the fixed tissue specimens in precisely localized areas for study using electron microscopy. This perfusion fixation technique provides both faster tissue harvesting capability and higher quality tissue preservation, without the artifacts of brain swelling and ventricular dilation observed in direct cardiac perfusion. Acute, discrete change in brain tissue can be studied.
Eichenbaum
Kenneth D
KD
New York University School of Medicine, New York, NY, USA.
Eichenbaum
Joseph W
JW
Fadiel
Ahmed
A
Miller
Douglas C
DC
Demir
Necdet
N
Naftolin
Frederick
F
Stern
Arnold
A
Pevsner
Paul H
PH
D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D003593:Cytoplasm / Q000378:metabolism; D005404:Fixatives; D006652:Histological Techniques; D051379:Mice; D008854:Microscopy, Electron; D008867:Microtomy; D009474:Neurons / Q000378:metabolism; D010477:Perfusion; D011309:Preservation, Biological; D016610:Tissue Embedding / Q000379:methods*; D014021:Tissue Preservation / Q000295:instrumentation* / Q000379:methods*
D005404:Fixatives