title: Cell culture in a closed nano-space.
abstract: The minimum size of a closed nano-space in which cells can survive was determined using 4-nl nanowells. One or two cells could divide in the nanowell. Our results suggest that the cell division activity in the nano-space is determined by the conflict between intercellular effects and consumption of substrates.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Doi
authors.forename: Sachihiro
authors.initials: S
authors.lastname: Matsunaga
authors.forename: Emi
authors.initials: E
authors.lastname: Maeno
authors.forename: Kensuke
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: Tsunehito
authors.initials: T
authors.lastname: Higashi
authors.forename: Shigeki
authors.initials: S
authors.lastname: Misawa
authors.forename: Susumu
authors.initials: S
authors.lastname: Uchiyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ooi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakao
authors.forename: Kiichi
authors.initials: K
authors.lastname: Fukui