title: Nicotinic acetylcholine receptors in rat and human placenta.
abstract: Smoking during pregnancy causes low birth weight, premature delivery, neonatal morbidity, and mortality. Nicotine is a main pathogenic compound of cigarette smoke, and depresses active amino-acid uptake by human placental villi. It binds to the acetylcholine binding site of the alpha-subunits of nicotinic acetylcholine receptors (nAChR). Eight different neuronal nAChR alpha-subunits have been identified in mammals. Here, we investigated their localisation and distribution in the human and rat placenta by RT-PCR and immunofluorescence. The mRNAs of all alpha-subunits are expressed in the human and rat placenta. Immunohistochemically, subunits alpha2-5, alpha7, alpha9 and alpha10 are localised in different combinations in rat cytotrophoblast, human and rat syncytiotrophoblast, vascular smooth muscle cells, endothelial cells, Hofbauer cells, human amnion epithelium and rat visceral yolk sac epithelium. Thus, all human and rat placental cell types exhibit receptor subunits with binding sites for the endogenous ligand ACh and nicotine. ACh is suggested to be an important placental signalling molecule that, through stimulation of nAChR, controls the uptake of nutrients, blood flow and fluid volume in placental vessels, and the vascularisation during placental development. Chronic stimulation of nAChR by nicotine might result in unbalanced receptor activation or functional desensitisation followed by the known pathological effects of smoking.
authors.affiliation: Institute for Anatomy and Cell Biology, Justus-Liebig-University, Aulweg 123, D-35385 Giessen, Germany. katrin.s.lips@anatomie.med.uni-giessen.de
authors.forename: K S
authors.initials: KS
authors.lastname: Lips
authors.forename: D
authors.initials: D
authors.lastname: Brüggmann
authors.forename: U
authors.initials: U
authors.lastname: Pfeil
authors.forename: R
authors.initials: R
authors.lastname: Vollerthun
authors.forename: S A
authors.initials: SA
authors.lastname: Grando
authors.forename: W
authors.initials: W
authors.lastname: Kummer
mesh_terms: D000818:Animals; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011978:Receptors, Nicotinic / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D011978:Receptors, Nicotinic