Difference in transcription levels of cap genes for gamma-polyglutamic acid production between Bacillus subtilis IFO 16449 and Marburg 168.
In a strain carrying capB-lacZ fusion of Bacillus subtilis IFO16449, which produces a large amount of gamma-polyglutamic acid (PGA), beta-galactosidase activity was enhanced by about five times with the addition of L-glutamic acid. This increase was also confirmed by Northern blot analysis. On the other hand, the activity was not detected in a strain carrying capB-lacZ fusion of B. subtilis Marburg 168. However, when the cap genes (capBCA and ywtC) were fused to the IPTG-inducible spac promoter, B. subtilis Marburg 168 produced PGA. These results suggest that the inability of B. subtilis Marburg 168 to produce PGA is due to defective expression of the cap genes.
Urushibata
Yuji
Y
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan.
Tokuyama
Shinji
S
Tahara
Yasutaka
Y