Metal bioaccumulation and metallothionein concentrations in larvae of Crassostrea gigas.
Larval stages of bivalve molluscs are highly sensitive to pollutants. Oysters from a hatchery from Normandy (English Channel) were induced to spawn, and fertilized eggs were exposed to copper or cadmium for 24 h. Metal accumulation (from 0.125 to 5 microg Cu L(-1) and from 25 to 200 microg Cd L(-1)) and MT concentrations were measured in larvae. Compared to controls, larvae accumulated copper and cadmium with an increase in MT concentrations particularly with cadmium (i.e. 130.96 ng Cu (mg protein)(-1) and 12.69 microg MT (mg protein)(-1) at 1 microg Cu L(-1) versus 23.19 ng Cu (mg protein)(-1) and 8.92 microg MT (mg protein)(-1) in control larvae; 334.3 ng Cd (mg protein)(-1) and 11.70 microg MT (mg protein)(-1) at 200 microg Cd L(-1) versus 0.87 ng Cd (mg protein)(-1) and 4.60 microg MT (mg protein)(-1) in control larvae). Larvae were also obtained from oysters of a clean area (Arcachon Bay) and a polluted zone (Bidassoa estuary) and exposed to copper in the laboratory, their MT concentration was measured as well as biomarkers of oxidative stress. Biomarker responses and sensitivity to copper for the larvae from Arcachon oysters were higher than for those from Bidassoa.
Damiens
Gautier
G
UMR 1112 INRA-UNSA R.O.S.E., Faculty of Sciences, Parc Valrose, B.P. 71, 06108 Nice Cedex 2, France.
Mouneyrac
Catherine
C
Quiniou
Françoise
F
His
Edouard
E
Gnassia-Barelli
Mauricette
M
Roméo
Michèle
M
D000818:Animals; D001682:Biological Availability; D015415:Biomarkers / Q000032:analysis; D002104:Cadmium / Q000032:analysis / Q000493:pharmacokinetics* / Q000633:toxicity; D003300:Copper / Q000032:analysis / Q000493:pharmacokinetics* / Q000633:toxicity; D049896:Crassostrea / Q000737:chemistry*; D004781:Environmental Exposure / Q000009:adverse effects; D004785:Environmental Pollutants / Q000032:analysis / Q000493:pharmacokinetics* / Q000633:toxicity; D007814:Larva / Q000737:chemistry; D008668:Metallothionein / Q000032:analysis*; D018384:Oxidative Stress; D012965:Sodium Chloride / Q000737:chemistry; D013696:Temperature; D014877:Water Pollution, Chemical / Q000009:adverse effects
D015415:Biomarkers; D004785:Environmental Pollutants; D002104:Cadmium; D012965:Sodium Chloride; D003300:Copper; D008668:Metallothionein