title: A convenient method for preparation of the calcium ion-binding protein annexin V.
abstract: This study presents a novel method for the production and purification of annexin V using cation exchange chromatography in the presence of calcium ions at neutral or alkaline pH. This method enables the handling of a large quantity of sample at one time without deterioration of the adsorption capacity of the cation exchange carrier by contaminating proteins, as well as the production of the calcium ion-binding protein annexin V with high purity on an industrial scale from both recombinant products and native products.
authors.affiliation: Applied Research Department, The Chemo-Sero-Therapeutic Research Institute (Kaketsuken), Kyokushi, Kikuchi, Kumamoto 869-1298, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizokami
authors.forename: Shinichi
authors.initials: S
authors.lastname: Furukawa
authors.forename: Keishin
authors.initials: K
authors.lastname: Sugawara
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Maeda
authors.forename: Tatsufumi
authors.initials: T
authors.lastname: Onchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Soejima
authors.forename: Satoshi
authors.initials: S
authors.lastname: Koyanagi
authors.forename: Hideo
authors.initials: H
authors.lastname: Yoshizaki
authors.forename: Chuichi
authors.initials: C
authors.lastname: Hirayama