title: [Alteration and its significance to expression of aquaporin-4 in cultured neonatal rat astrocytes in the model of hypoxic damage].
abstract: OBJECTIVE
  To investigate the expression of aquaorin-4 (AQP4) in the cultured neonatal rat primary astrocytes during hypoxia and during reoxygenation after hypoxia.
  
  
  METHODS
  The astrocytes were randomly assigned to the hypoxia group (the astrocytes were exposed to hypoxia for 3 h, 6 h, 12 h and 24 h respectively), the reoxygenation group (after exposure to 12 h hypoxia, the astrocytes were returned to normoxic cultures for 3 h, 6 h, 12 h and 24 h respectively), and the normoxic group (control group). AQP4 mRNA expression in astrocytes of all groups was detected by Real Time PCR, and AQP4 protein expression was determined by immunofluorescence.
  
  
  RESULTS
  The AQP4 mRNA and protein expressions significantly decreased with the time of hypoxia, and during reoxygenation, the decreased expressions were gradually up-reguated.
  
  
  CONCLUSIONS
  It is likely that the decreased expression of AQP4 is associated with brain edema and in such brain edema conditions AQP4 may play a prominent role in the reestablishment of brain osmotic equilibrium.
authors.affiliation: Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Fu
authors.forename: Yu-Jia
authors.initials: YJ
authors.lastname: Yao
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
authors.forename: Long
authors.initials: L
authors.lastname: Xiang
authors.forename: Ju
authors.initials: J
authors.lastname: Gao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D051401:Aquaporin 4 / Q000096:biosynthesis* / Q000235:genetics; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology; D001921:Brain / Q000378:metabolism / Q000473:pathology; D015687:Cell Hypoxia / Q000502:physiology; D002478:Cells, Cultured; D002534:Hypoxia, Brain / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D015854:Up-Regulation
chemical_list: D051401:Aquaporin 4; D012333:RNA, Messenger