Synthesis and application of mono-6-ammonium-6-deoxy-beta-cyclodextrin chloride as chiral selector for capillary electrophoresis.
A facile synthetic approach for mono-6-amino-6-deoxy-beta-cyclodextrin (beta-CD-NH2) was proposed. Its hydroxy chloride salt, mono-6-ammonium-6-deoxy-beta-cyclodextrin chloride (beta-CD-NH3Cl) was further prepared and used for the enantioseparation of various anionic and ampholytic analytes by capillary electrophoresis (CE). The effect of background electrolyte (BGE) pH and selector concentration on the enantioseparation was studied. Results showed that beta-CD-NH3Cl displayed powerful chiral resolution ability towards anionic analytes. In addition, baseline separation of a standard mixture consisting of eight acids was achieved within 35 min.
Tang
Weihua
W
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
Muderawan
I Wayan
IW
Ng
Siu Choon
SC
Chan
Hardy Sze On
HS
D019075:Electrophoresis, Capillary / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
D000644:Quaternary Ammonium Compounds; D047392:beta-Cyclodextrins; C507824:mono-6-ammonium-6-deoxy-beta-cyclodextrin chloride