Reciprocal interference between specific CJD and scrapie agents in neural cell cultures.
Infection of mice with an attenuated Creutzfeldt-Jakob disease agent (SY-CJD) interferes with superinfection by a more virulent human-derived CJD agent (FU-CJD) and does not require pathological prion protein (PrPres). Using a rapid coculture system, we found that a neural cell line free of immune system cells similarly supported substantial CJD agent interference without PrPres. In addition, SY-CJD prevented superinfection by sheep-derived Chandler (Ch) and 22L scrapie agents. However, only 22L and not Ch prevented FU-CJD infection, even though both scrapie strains provoked abundant PrPres. This relationship between particular strains of sheep- and human-derived agents is likely to affect their prevalence and epidemic spread.
Nishida
Noriuki
N
Yale Medical School, New Haven, CT 06510, USA.
Katamine
Shigeru
S
Manuelidis
Laura
L
D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D007562:Creutzfeldt-Jakob Syndrome; D006801:Humans; D051379:Mice; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D018620:PrPSc Proteins / Q000378:metabolism / Q000472:pathogenicity*; D011328:Prions / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology*; D012608:Scrapie; D012756:Sheep; D013045:Species Specificity; D014774:Virulence
D018620:PrPSc Proteins; D011328:Prions