Hydrogenase genes are uncommon and highly conserved in Rhizobium leguminosarum bv. viciae.
A screening for hydrogen uptake (hup) genes in Rhizobium leguminosarum bv. viciae isolates from different locations within Spain identified no Hup+ strains, confirming the scarcity of the Hup trait in R. leguminosarum. However, five new Hup+ strains were isolated from Ni-rich soils from Italy and Germany. The hup gene variability was studied in these strains and in six available strains isolated from North America. Sequence analysis of three regions within the hup cluster showed an unusually high conservation among strains, with only 0.5-0.6% polymorphic sites, suggesting that R. leguminosarum acquired hup genes de novo in a very recent event.
Fernández
Domingo
D
Laboratorio de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria, s/n, 28040 Madrid, Spain.
Toffanin
Annita
A
Palacios
José Manuel
JM
Ruiz-Argüeso
Tomás
T
Imperial
Juan
J
D001483:Base Sequence; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000235:genetics; D019143:Evolution, Molecular; D007887:Fabaceae / Q000382:microbiology; D005798:Genes, Bacterial; D006864:Hydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids / Q000235:genetics; D011110:Polymorphism, Genetic; D016961:Rhizobium leguminosarum / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity; D013559:Symbiosis
D004269:DNA, Bacterial; D006864:Hydrogenase