title: Sterilisation in biotechnology.
abstract: Sterilisation of fermentation media, inlet and outlet gases and maintenance of aseptic conditions during bioprocessing represents one of the most relevant parts of comprehensive upstream processing. The present chapter reviews various sterilisation techniques and methods for sterilisation of inlet gases, liquids, including sterilisation of small and large industrial equipment and validation of sterilisation procedures. Various methods for sterilisation using different kinds of filters, filter cartridges and membrane filters are also presented. Liquid sterilisation methods by filtration as well as heat sterilisation are discussed. Sterilisation of small and industrial scale equipment by chemical agents and physical methods as ionising radiation and dry heat sterilisation including the validation of sterilisation are discussed.
authors.affiliation: Faculty of Chemistry and Chemical Engineering, Department of Chemical, Biochemical and Environmental Engineering, University of Ljubljana, Askerceva 5, 1000 Ljubljana, Slovenia. marin.berovic@fkkt.uni-lj.si
authors.forename: Marin
authors.initials: M
authors.lastname: Berovic
mesh_terms: D000465:Algorithms; D001709:Biotechnology / Q000379:methods*; D005374:Filtration / Q000379:methods; D006358:Hot Temperature; D011839:Radiation, Ionizing; D015203:Reproducibility of Results; D013242:Sterilization / Q000295:instrumentation / Q000379:methods* / Q000592:standards