title: Diffusion tensor tractography of the limbic system.
abstract: OBJECTIVE
  The limbic system, relevant to memory and emotion, is an interesting subject of study in healthy and diseased individuals. It consists of a network of gray matter structures interconnected by white matter fibers. Although gray matter components of this system have been studied by using MR imaging, the connecting fibers have not been analyzed to the same degree. Cerebrospinal fluid (CSF) signal intensity contamination of the fornix and cingulum, the 2 major white matter tracts of the limbic system, can alter diffusion-tensor imaging (DTI) measurements and affect tractography. We investigated the effect of CSF signal intensity suppression on fiber tracking of the limbic connections and characterized the diffusion properties of these structures in healthy volunteers.
  
  
  METHODS
  Nine healthy individuals were scanned with standard and CSF-suppressed DTI. Tractography of the fornix and cingulum was performed for both acquisition methods. We report mean diffusivity and fractional anisotropy measurements of the crus, body, and columns of the fornix, and descending, superior, and anterior portions of the cingulum.
  
  
  RESULTS
  Diffusion measurements were improved and tractography was facilitated by using CSF-suppressed DTI. In particular, tract volume increased, whereas decreases of the mean diffusivity and increases of diffusion anisotropy more accurately represented the underlying tissue by minimizing deleterious partial volume averaging from CSF. This was particularly true for the fornix because it is in closest contact to CSF. Diffusion measurements throughout the limbic connections were consistent in healthy volunteers.
  
  
  CONCLUSIONS
  We recommend the use of CSF suppression when performing diffusion-tensor tractography of the limbic system.
authors.affiliation: Department of Biomedical Engineering University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Luis
authors.initials: L
authors.lastname: Concha
authors.forename: Donald W
authors.initials: DW
authors.lastname: Gross
authors.forename: Christian
authors.initials: C
authors.lastname: Beaulieu
mesh_terms: D000328:Adult; D016880:Anisotropy; D002555:Cerebrospinal Fluid; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods; D020712:Fornix, Brain / Q000033:anatomy & histology; D006801:Humans; D008032:Limbic System / Q000033:anatomy & histology*; D009412:Nerve Fibers; D009434:Neural Pathways