title: Human parainfluenza virus 3 neuraminidase activity contributes to dendritic cell maturation.
abstract: Mechanisms of dendritic cells (DCs) immunomodulation by parainfluenza viruses have not been characterized. We analyzed whether the human parainfluenza 3 (HPF3) virus hemagglutinin-neuraminidase glycoprotein (HN) might influence DC maturation. HN possesses a receptor binding function and a neuraminidase or desialidating activity. To assess whether the neuraminidase activity of HN affects DC maturation, human myeloid DCs were exposed to either live or UV-inactivated HPF3 viruses containing wild type or a mutated form of HN with decreased neuraminidase activity. Exposure of human DCs to either UV-inactivated or live virus induced up-regulation of CD83 and CD86 surface markers, morphological changes, and a cytokine expression pattern consistent with maturation. However, the level of maturation was found to be lower in DCs infected with the neuraminidase deficient variant as compared to the wild type. These results suggest that during the course of viral infection, HN's neuraminidase activity may play an important role contributing to maturation and activation of DCs.
authors.affiliation: Department of Pediatric Infectious Diseases, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1657, New York, NY 10029, USA. maria.horga@mssm.edu
authors.forename: Maria-Arantxa
authors.initials: MA
authors.lastname: Horga
authors.forename: Salvador
authors.initials: S
authors.lastname: Macip
authors.forename: Ana C
authors.initials: AC
authors.lastname: Tuyama
authors.forename: Maria C
authors.initials: MC
authors.lastname: Tan
authors.forename: G Luca
authors.initials: GL
authors.lastname: Gusella
mesh_terms: D002454:Cell Differentiation; D018925:Chemokines / Q000096:biosynthesis; D003016:Clostridium perfringens / Q000201:enzymology / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology*; D005814:Genes, Viral; D006801:Humans; D066298:In Vitro Techniques; D009154:Mutation; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology*; D010224:Parainfluenza Virus 3, Human / Q000201:enzymology* / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D010253:Respirovirus Infections / Q000276:immunology / Q000473:pathology; D013045:Species Specificity
chemical_list: D018925:Chemokines; D016207:Cytokines; D009439:Neuraminidase