Detection of Epstein Barr virus in formalin-fixed paraffin tissues by fluorescent direct in situ PCR.
Specific viral laboratory diagnosis of primary Epstein-Barr Virus (EBV) infection is usually based on antibody-detection assays. However, molecular detection is also considered the reference standard assay for diagnosis of central nervous system infections and of most cases of nasopharyngeal carcinoma (NPC). One-step or nested polymerase chain reaction (PCR) has rapidly replaced immunological assays based on virus-specific Ig antibodies for the laboratory diagnosis of Herpesvirus infections, even if serological methods are considered an additional tool for defining clinical diagnosis. In this article, we will present a rapid, sensitive and robust molecular tool for the viral detection of EBV (EBNA-1) within tissue specimens by making use of in situ PCR (IS-PCR).
Marziliano
Nicola
N
Transplant Research Area, Molecular Diagnostic Cardiovascular and Transplant Pathology Laboratory, IRCCS Policlinico S Matteo, Piazzale Golgi 1, 27100 Pavia, Italy. n.marziliano@smatteo.pv.it
Arbustini
E
E
Rossi de Gasperis
M
M
Crovella
S
S
D005557:Formaldehyde / Q000737:chemistry; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
D005557:Formaldehyde