Effect of intrathecal administration of hemokinin-1 on the withdrawal response to noxious thermal stimulation of the rat hind paw.
Hemokinin-1 (HK-1) is a new peptide described as a member of the tachykinin family. HK-1 has biological effects similar to substance P (SP), a representative of the tachykinin family, following central administration. However, the biological function of HK-1 at the spinal level has not been well characterized. Thus, we investigated the effect of intrathecal administration of HK-1 by comparing it with that of SP. Intrathecal administration of HK-1 as well as SP at 10(-3) M caused pain-related behavior such as scratching. The scratching by HK-1 administration was inhibited by pretreatment with an antagonist of substance P receptor. In addition, SP (10(-8)-10(-6) M) decreased the latency of the withdrawal response of the hind paw to noxious thermal stimulation 20-30 min after intrathecal administration, whereas administration of HK-1 had little effect on this response. These results suggest that there may exist a proper receptor related to HK-1.
Endo
Daisuke
D
Division of Neurobiology, Miyazaki Medical College, University of Miyazaki, Kiyotake, Japan.
Ikeda
Tetsuya
T
Ishida
Yasushi
Y
Yoshioka
Daisuke
D
Nishimori
Toshikazu
T
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004347:Drug Interactions; D006614:Hindlimb / Q000187:drug effects / Q000503:physiopathology / Q000528:radiation effects; D006358:Hot Temperature / Q000009:adverse effects; D008297:Male; D010146:Pain / Q000523:psychology*; D010147:Pain Measurement / Q000187:drug effects; D010446:Peptide Fragments / Q000008:administration & dosage*; D011812:Quinuclidines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D013373:Substance P / Q000008:administration & dosage; D015320:Tachykinins / Q000008:administration & dosage*; D013997:Time Factors
D010446:Peptide Fragments; D011812:Quinuclidines; D015320:Tachykinins; C507968:hemokinin-1; C076851:L 703606; D013373:Substance P