title: Processing of membrane protein crystal using ultraviolet laser irradiation.
abstract: We demonstrated the processing of a membrane protein crystal, using a pulsed UV laser soft ablation (PULSA) technique. Irradiation with deep-UV laser pulses at a wavelength of 193 nm successfully processed not only single crystals of the membrane transporter protein AcrB but also nylon loops and cryoprotectants at a cryogenic temperature. Nonprocessed parts of the crystals exhibited no signs of crack or denaturation after the laser exposure. The trimmed crystals were found to be of high resolution for X-ray diffraction data collection. The results described here indicate that PULSA processing is an effective tool for membrane protein crystals, as well as for soluble protein crystals.
authors.affiliation: Department of Electrical Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Murakami
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Adachi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Takano
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Mori
authors.forename: Masaaki
authors.initials: M
authors.lastname: Doi
authors.forename: Takatomo
authors.initials: T
authors.lastname: Sasaki
mesh_terms: D002352:Carrier Proteins / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D003460:Crystallization / Q000379:methods*; D029968:Escherichia coli Proteins / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D007834:Lasers; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D046912:Multiprotein Complexes / Q000032:analysis / Q000737:chemistry / Q000528:radiation effects / Q000648:ultrastructure; D011208:Powders; D014466:Ultraviolet Rays
chemical_list: D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D046912:Multiprotein Complexes; D011208:Powders; C083496:AcrB protein, E coli