Kinetics of active sucrose transport in Saccharomyces cerevisiae.
The kinetic analysis of active sucrose-H+ uptake by Saccharomyces cerevisiae revealed the presence of two transport systems with high and low affinity for sucrose. The MAL2T permease has a low affinity (K(m) = 120 +/-20 mM) for sucrose, while the alpha-glucoside transporter encoded by the AGT1 gene is a high affinity sucrose-H+ symporter (K(m) = 7.9+/-0.8 mM) that increases the specific growth rate of cells growing on sucrose.
Stambuk
B U
BU
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, Brazil.
Batista
A S
AS
De Araujo
P S
PS