Organic solvent tolerance of halophilic archaea, Haloarcula strains: effects of NaCl concentration on the tolerance and polar lipid composition.
Strains of halophilic archaea, Haloarcula vallismortis and two Haloarcula strains OHF-1 and OHF-2, showed high tolerance to organic solvents at high media NaCl concentrations. For example, the lowest log Pow of the solvent which allowed growth (log Pow is the common logarithm of the partition coefficient of a given solvent in a mixture of n-octanol and water) for H. vallismortis was 5.1 at 20% NaCl and 4.4 at 30% NaCl. The solvent tolerance of Haloarcula argentinensis, on the other hand, was not affected by the NaCl concentration. Cells of strains OHF-1 and OHF-2 were of triangular or irregular morphology but became spherical in cultures in NaCl media overlaid with cyclohexane (log Pow=3.4), but returned to the triangular shape when the organic solvent evaporated from the medium. When cells of strains OHF-1, OHF-2, and H. argentinensis were grown in NaCl media in the presence of n-decane, they contained less phosphatidylglycerol and more phosphatidylglycerosulfate and phosphatidylglycerophosphate methyl ester than when grown without added n-decane. When the solvent was removed from the media after cultivation, the levels of these compounds returned to their initial ones.
Usami
Ron
R
Department of Applied Chemistry, Faculty of Engineering, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama 350-8585, Japan. usaron@mail.cc.eng.toyo.ac.jp
Fukushima
Tadamasa
T
Mizuki
Toru
T
Yoshida
Yasuhiko
Y
Inoue
Akira
A
Horikoshi
Koki
K
D049109:Cell Proliferation; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000502:physiology*; D016931:Halobacteriales / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism; D009930:Organic Chemicals / Q000008:administration & dosage*; D012965:Sodium Chloride / Q000008:administration & dosage*; D012997:Solvents / Q000008:administration & dosage*; D013045:Species Specificity
D009930:Organic Chemicals; D012997:Solvents; D012965:Sodium Chloride