title: Erythropoietin unfolding: thermodynamics and its correlation with structural features.
abstract: Human erythropoietin (EPO) is a glycoprotein hormone considered to be the principal regulator of red blood cell formation. Although its recombinant version (rEPO) has been widely used for treatment of various anemias and its biological effects are relatively well-known, we know little about its biophysical properties and their relation to its structure. To gain a fuller understanding of the structural and functional properties of rEPO on the molecular level we followed its thermal and urea-induced unfolding at different pH (3.1-9.4) and urea concentrations (0-8 M) using spectropolarimetry, UV absorption, intrinsic emission fluorescence, and differential scanning calorimetry. Our results show that under a variety of conditions rEPO undergoes thermal or urea-induced denaturation that may be considered as a reversible two-state process characterized by unusually high (thermal) or moderate (urea-induced) extent of the residual structure. The highest thermal stability of the protein observed in aqueous solutions at physiological pH appears to be due to the largest difference in the extent of structure in the denatured and native state at this pH. The comparison between experimentally determined energetics of rEPO denaturation and its structure-based calculations indicates that the parametrization of thermodynamic quantities in terms of changes in solvent accessible nonpolar and polar surface areas resulting from protein unfolding can be successfully used provided that these changes are estimated from combination of experimentally determined deltaC(o)p and deltaH(o) values and not calculated from the structure of the protein's folded and assumingly fully unfolded state.
authors.affiliation: Faculty of Chemistry and Chemical Technology, University of Ljubljana, Askerceva 5, 1000 Ljubljana, Slovenia. jurij.lah@fkkt.uni-lj.si
authors.forename: Jurij
authors.initials: J
authors.lastname: Lah
authors.forename: Iztok
authors.initials: I
authors.lastname: Prislan
authors.forename: Blaz
authors.initials: B
authors.lastname: Krzan
authors.forename: Mateja
authors.initials: M
authors.lastname: Salobir
authors.forename: Andrej
authors.initials: A
authors.lastname: Francky
authors.forename: Gorazd
authors.initials: G
authors.lastname: Vesnaver
mesh_terms: D002152:Calorimetry, Differential Scanning; D004921:Erythropoietin / Q000737:chemistry*; D006801:Humans; D011487:Protein Conformation; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D004921:Erythropoietin