title: Cloning and expression of an ether-bond-cleaving enzyme involved in the metabolism of polyethylene glycol.
abstract: A gene encoding an ether-bond-cleaving enzyme, diglycolic acid dehydrogenase (DGADH) from polyethylene glycol-utilizing Pseudonocardia sp. strain K1, was cloned and expressed in Escherichia coli. The deduced amino acid sequence had a high homology with superoxide dismutases (SODs) from various bacteria. The recombinant protein showed the same activities as those of DGADH from Pseudonocardia sp. strain K1, namely, SOD activity and ether-bond-cleaving activity.
authors.affiliation: Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamashita
authors.forename: Akio
authors.initials: A
authors.lastname: Tani
authors.forename: Fusako
authors.initials: F
authors.lastname: Kawai