Identification of unknown quaternary ammonium compounds in corneal epithelium and aqueous humor.
Bovine corneal epithelium and bovine aqueous humor are investigated for their content of quaternary ammonium compounds. In total, four compounds are found. Three of these are identified. For the fourth compound, a proposal for its structure is made on the basis of tandem mass spectrometry fragmentation spectra. The compounds investigated have m/z values of 146, 160, and 174. The compounds with m/z 146 are confirmed as acetylcholine (in corneal epithelium) and (3-carboxypropyl)-trimethylammonium (in both corneal epithelium and aqueous humor). The compound with m/z 174 is identified as butyrylcholine (in corneal epithelium). The compound with m/z 160 is probably acetyl-g-homocholine (in both corneal epithelium and aqueous humor). For both butyrylcholine and acetyl-g-homocholine, it is the first time the presence of these compounds in corneal epithelium or aqueous humor (or both) is described. Both acetylcholine and butyrylcholine are unstable compounds, which are probably susceptible to enzymatic degradation by acetylcholine-esterase and butytrylcholine- esterase, respectively.
Reubsaet
J L E
JL
Department of Pharmaceutical Analysis, School of Pharmacy, University of Oslo, Norway.
Ringvold
A
A
D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D019573:Epithelium, Corneal / Q000737:chemistry*; D013058:Mass Spectrometry; D000644:Quaternary Ammonium Compounds / Q000032:analysis*
D000644:Quaternary Ammonium Compounds