[Construction of a full-length cDNA library of Aegilops speltoides Tausch with optimized cap-trapper method].
To discover new genes in a throughput manner,the cap-trapper method published previously was optimized for raising the efficiency in the construction of full-length cDNA library. Using the optimized protocol,we successfully constructed a full-length cDNA library of Aegilops speltoides,which contained 3.0 x 10(6) clones and more than 99% of plaques were recombinant phages. Sequence analysis results indicated that more than 89% of the clones were full-length.
Mao
Xin-Guo
XG
Key Laboratory of Crop Germplasm & Biotechnology/the Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081, China. maoxg2001@sina.com
Kong
Xiu-Ying
XY
Zhao
Guang-Yao
GY
Jia
Ji-Zeng
JZ
D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D006109:Poaceae / Q000235:genetics*; D012315:RNA Caps; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012315:RNA Caps; D012333:RNA, Messenger; D018749:RNA, Plant