Peculiarities [correction of Pfculiarities] of lipid accumulation in Brassica rapa L. embryos on different stages development under altered gravity.
Accumulation of lipid inclusions in Brassica rapa embryos generated under slow horizontal clinorotation and in the laboratory control were analyzed by histochemical methods. The research of lipid accumulation was carried out on consecutive stages of the embryo development, from the moment of two-cellular proembryo formation up to the stages of their full differentiation (21-22-day-old embryos). Accumulation of lipid drops was revealed for the first time at early stages of embryogenesis in this species, beginning from 3-day-old embryos (ball-like stage of embryo development) under clinorotation and in the laboratory control. The quantity of lipid inclusion was estimated by morphometrical analysis. Statistically significant differences between the clinorotation and laboratory control variants in quantity of lipid drops per cell were revealed from 6-day-old embryos (heart-shaped stage). Especially pronounced differences were noted in differentiated embryos (beginning from 12-day-old embryos) under horizontal clinorotation in comparison with the laboratory control. The registered differences testify about influence of altered gravity conditions on lipid accumulation in Brassica rapa embryos.
Popova
Antonina
A
Institute of Botany, National Academy of Science of Ukraine, Kiev, Ukraine. cell@svitonlne.com
Kononko
Anna
A
Ivanenko
Galina
G
D029741:Brassica rapa / Q000196:embryology* / Q000378:metabolism; D018548:Cotyledon / Q000196:embryology* / Q000378:metabolism; D050356:Lipid Metabolism; D012399:Rotation; D012639:Seeds / Q000196:embryology* / Q000378:metabolism; D018474:Weightlessness Simulation