Identification of secreted proteins of Aspergillus oryzae associated with growth on solid cereal substrates.
Filamentous growth of Aspergillus oryzae on solid cereal substrates involves secretion of substrate converting enzymes and a solid substrate specific polarised hyphal growth phenotype. To identify proteins produced under these specific conditions, the extracts of A. oryzae grown on wheat-based media were analysed using N-terminal sequence analysis. In a submerged wheat-based growth medium of A. oryzae, besides alpha-amylase, also an arabinosidase and xylanase were abundantly produced. In the extracts of A. oryzae grown on wheat-based solid substrate besides alpha-amylase and chitinase, two new proteins of 16 and 27 kDa were identified. These hypothetical proteins showed only close homologies to filamentous fungal proteins.
Te Biesebeke
R
R
Wageningen Centre for Food Sciences (WCFS), P.O. Box 557, 6700 AN, Wageningen, The Netherlands. tebiesebeke@yahoo.com
Boussier
A
A
van Biezen
N
N
van den Hondel
C A M J J
CA
Punt
P J
PJ
D001236:Aspergillus oryzae / Q000254:growth & development*; D002523:Edible Grain / Q000382:microbiology; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism*
D005656:Fungal Proteins