Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane.
The E-Cadherin-catenin complex plays a critical role in epithelial cell-cell adhesion, polarization, and morphogenesis. Here, we have analyzed the mechanism of Drosophila E-Cadherin (DE-Cad) localization. Loss of function of the Drosophila exocyst components sec5, sec6, and sec15 in epithelial cells results in DE-Cad accumulation in an enlarged Rab11 recycling endosomal compartment and inhibits DE-Cad delivery to the membrane. Furthermore, Rab11 and Armadillo interact with the exocyst components Sec15 and Sec10, respectively. Our results support a model whereby the exocyst regulates DE-Cadherin trafficking, from recycling endosomes to sites on the epithelial cell membrane where Armadillo is located.
Langevin
Johanna
J
Polarité Cellulaire chez la drosophile, UMR 144, Paris, France.
Morgan
Matthew J
MJ
Sibarita
Jean-Baptiste
JB
Aresta
Sandra
S
Murthy
Mala
M
Schwarz
Thomas
T
Camonis
Jacques
J
Bellaïche
Yohanns
Y
D000818:Animals; D051186:Armadillo Domain Proteins; D015820:Cadherins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D004330:Drosophila / Q000378:metabolism / Q000648:ultrastructure; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005089:Exocytosis / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins; D008954:Models, Biological; D009865:Oocytes / Q000378:metabolism* / Q000648:ultrastructure; D021381:Protein Transport / Q000502:physiology; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
C495305:ARM protein, Drosophila; D051186:Armadillo Domain Proteins; D015820:Cadherins; D002352:Carrier Proteins; D029721:Drosophila Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C505147:Sec15 protein, Drosophila; C502950:Sec5 protein, Drosophila; C502606:Sec6 protein, Drosophila; D015534:Trans-Activators; D014157:Transcription Factors; D033921:Vesicular Transport Proteins; C474345:sec10 protein, Drosophila