Rapid one-step purification of the BChl-a containing FMO-protein from the green sulfur bacterium Chlorobium tepidum using a high efficiency immunomatrix.
A new and rapid procedure has been developed for the isolation of the bacteriochlorophyll a-containing Fenna-Matthews-Olson (FMO)-protein from green sulfur bacteria. Polyclonal antibodies raised against the FMO-protein of Chlorobium (Chl.) tepidum were employed in the preparation of an antibody column utilizing immobilized protein A as the matrix. The antibody column afforded essentially a one-step purification process, resulting in preparations that were free from contaminating pigments and proteins. This was evidenced by absorption spectroscopy, SDS-PAGE, and fluorescence emission.
Hu
Diane D
DD
Department of Chemistry and Biochemistry, Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe, AZ, 85287-1604, USA, blankenship@asu.edu.
Blankenship
Robert E
RE