Optimization of aeration and agitation rates to improve cellulase-free xylanase production by thermotolerant Streptomyces sp. Ab106 and repeated fed-batch cultivation using agricultural waste.
Thermostable cellulase-free xylanase was produced by Streptomyces sp. Ab106 using agricultural waste, sugar cane bagasse, as the substrate at 50 degrees C and pH 7.0. The central composite face-centered experimental design was applied to evaluate the optimal agitation and aeration rates in a 5-l fermentor. The highest activity (16.0+/-0.5 IU/ml) was obtained at an aeration rate of 1 vvm and an agitation rate of 150 rpm (k(L)a = 351 h(-1)). Using the repeated fed-batch cultivation technique, the maximum xylanase activity of 32+/-1 IU/ml was obtained during the second cycle of repeated fed-batch culture.
Techapun
Charin
C
Department of Biotechnology, Faculty of Agro-Industry, Chiangmai University, Chiangmai 50100, Thailand.
Poosaran
Naiyatat
N
Watanabe
Masanori
M
Sasaki
Ken
K