Assessment of the phototoxic hazard of some essential oils using modified 3T3 neutral red uptake assay.
When substances are developed in the aim to be a constituent of personal care products, and to be applied on the skin, it is necessary to carry out an assessment of potential phototoxic hazard. Phototoxicity is skin reaction caused by concurrent topical or systemic exposure to specific molecule and ultraviolet radiation. Most phototoxic compounds absorb energy particularly from UVA light leading to the generation of activated derivatives which can induce cellular damage. This type of adverse cutaneous response can be reproduced in vitro using different models of phototoxicity such as the validated 3T3 Neutral Red Uptake (NRU) phototoxicity assay. In the present study we utilised two different cell lines (the murine fibroblastic cell line 3T3 and the rabbit cornea derived cell line SIRC) to compare the photo-irritation potential of a strong phototoxic compound, chlorpromazine, to a weaker composite, such as 8-methoxypsoralen and Bergamot oil. After comparison of the different systems, five other essential oils were tested with both cell lines. Cellular damage was evaluated by the NRU cytotoxicity test or by MTT conversion test.
Dijoux
Nathalie
N
Yves-Rocher Research and Development, Worldwide Safety Department, 101 quai du Président Roosevelt, 92444 Issy les Moulineaux, France.
Guingand
Yannick
Y
Bourgeois
Caroline
C
Durand
Sandrine
S
Fromageot
Claude
C
Combe
Corinne
C
Ferret
Pierre-Jacques
PJ
D000818:Animals; D041702:BALB 3T3 Cells / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002746:Chlorpromazine / Q000633:toxicity; D003315:Cornea / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D003358:Cosmetics / Q000633:toxicity; D008730:Methoxsalen / Q000633:toxicity*; D051379:Mice; D009499:Neutral Red / Q000378:metabolism; D009822:Oils, Volatile / Q000737:chemistry / Q000633:toxicity; D010476:Perfume; D017319:Photosensitizing Agents / Q000145:classification / Q000633:toxicity*; D010938:Plant Oils / Q000633:toxicity*; D011817:Rabbits; D018675:Toxicity Tests
D003358:Cosmetics; D009822:Oils, Volatile; D010476:Perfume; D017319:Photosensitizing Agents; D010938:Plant Oils; D009499:Neutral Red; C068336:bergamot oil; D002746:Chlorpromazine; D008730:Methoxsalen