title: [PCR amplification, cloning and protein expression of interferon-inducible transmembrane protein-1 gene].
abstract: OBJECTIVE
  To study the PCR amplification, cloning and protein expression of interferon-inducible transmembrane protein-1 (IFITMP-1) gene.
  
  
  METHODS
  With the cDNA fragment containing IFITMP-1 gene as template, IFITMP-1 gene was amplified using Pfu enzyme by means of PCR. After EcoRI and HindIII digestion, the target gene fragment was linked to pUCm-T plasmid and sequenced. The IFITMP-1 gene was cloned into pET-Trx protein expression plasmid, and the condition for protein expression was optimized.
  
  
  RESULTS
  The length of the PCR product of IFITMP-1 gene-containing cDNA fragment was about 1000 bp. The IFITMP-1 gene was successfully inserted into pUCm-T plasmid with correct sequence and cloning of the IFITMP-1 gene into the pET-Trx protein expression plasmid was achieved. Expression of the fusion protein of pUCm-T plasmid and IFITMP-1 gene was detected after IPTG induction.
  
  
  CONCLUSIONS
  Successful amplification and cloning of the IFITMP-1 gene and its protein expression may facilitate further study of the role of IFITMP-1 gene in colorectal cancer.
authors.affiliation: Department of Digestive Diseases, People's Hospital of Dongguan, Dongguan 523018, China. liuyuhu@126.com
authors.forename: Yu-hu
authors.initials: YH
authors.lastname: Liu
authors.forename: Dong
authors.initials: D
authors.lastname: Zhong
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Zhen-shu
authors.initials: ZS
authors.lastname: Zhang
authors.forename: Cun-long
authors.initials: CL
authors.lastname: Chen
authors.forename: Jin-bao
authors.initials: JB
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Xiao
authors.forename: Wen-ying
authors.initials: WY
authors.lastname: Guo
mesh_terms: D000943:Antigens, Differentiation; D003001:Cloning, Molecular; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000943:Antigens, Differentiation; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C062010:leu-13 antigen; D007372:Interferons