title: Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR.
abstract: This study aimed to determine the occurrence of Vibrio parahaemolyticus in cockles (Anadara granosa) at a harvesting area and to detect the presence of virulent strains carrying the thermostable direct hemolysin (tdh) and TDH-related hemolysin genes (trh) using PCR. Of 100 samples, 62 were positive for the presence of V. parahaemolyticus with an MPN (most probable number) value greater than 3.0 (>1100 MPN per g). The PCR analysis revealed 2 samples to be positive for the tdh gene and 11 to be positive for the trh gene. Hence, these results demonstrate the presence of pathogenic V. parahaemolyticus in cockles harvested in the study area and reveal the potential risk of illness associated with their consumption.
authors.affiliation: Department of Food Science, Faculty of Food Science and Technology, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. lmaurice@hotmail.com
authors.forename: Lesley Maurice
authors.initials: LM
authors.lastname: Bilung
authors.forename: Son
authors.initials: S
authors.lastname: Radu
authors.forename: Abdul Rani
authors.initials: AR
authors.lastname: Bahaman
authors.forename: Raha Abdul
authors.initials: RA
authors.lastname: Rahim
authors.forename: Suhaimi
authors.initials: S
authors.lastname: Napis
authors.forename: Michael Wong Clemente Vui
authors.initials: MW
authors.lastname: Ling
authors.forename: Gwendelynne Bulan
authors.initials: GB
authors.lastname: Tanil
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Nishibuchi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics; D049872:Bivalvia / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004784:Environmental Monitoring / Q000379:methods; D006460:Hemolysin Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D012758:Shellfish / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; C088609:thermostable direct hemolysin-related hemolysin protein, Vibrio parahaemolyticus; C068489:thermostable direct hemolysin