title: Purification and characterization of a protease from Pseudomonas aeruginosa grown in cutting oil.
abstract: The Pseudomonas aeruginosa san-ai strain was isolated from water-soluble cutting oil used for cooling and lubrication during industrial metal-working processes. This strain, which is grown in a high alkaline (pH 10) mixture of surfactants and mineral oil, produces an extracellular proteolytic enzyme. We have purified and characterized this 18 kDa protease. The P. aeruginosa san-ai protease functions optimally at pH 9.0 and 60 degrees C. Additionally, it is a Zn-containing metalloenzyme, and its monomeric structure contains at least one disulfide bond. Because the enzyme is stable in the presence of organic solvents, it is suitable for peptide synthesis. Furthermore, the P. aeruginosa san-ai protease could be used in an intelligent drug delivery system (DDS) designed for applications in the metal industry for prevention of putrefaction of cutting oil.
authors.affiliation: School of Medicine, Department of Chemistry, Visegradska 26, 11000 Belgrade, Serbia and Montenegro.
authors.forename: Ivanka
authors.initials: I
authors.lastname: Karadzic
authors.forename: Akihiko
authors.initials: A
authors.lastname: Masui
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Fujiwara