[Expression specificity of hpt gene in sck transgenic rice].
OBJECTIVE
Evaluate the expression specificity of hpt gene in different developmental stages of sck transgenic rice.


METHODS
The different tissues, including leaves, culms, roots and seeds (ripening stage), in different developmental stages of sck transgenic rice were detected by PCR. The positive plants and the control plants were detected by Northern blot analysis and double sandwich ELISA test.


RESULTS
The hpt gene expression was found at transcription level and translation level in different tissues of sck transgenic rice. HPT protein in the seeds could not be detected.


CONCLUSIONS
The hpt gene in different developmental stages of sck transgenic rice express stably at transcription level and translation level and HPT protein in the seeds could not be detected.
Wang
Rui
R
National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Chen
Song-Biao
SB
Zhuo
Qin
Q
Yang
Li-Chen
LC
Zhu
Zhen
Z
Yang
Xiaoguang
X
D018506:Gene Expression Regulation, Plant; D005819:Genetic Markers; D012275:Oryza / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D012639:Seeds / Q000378:metabolism
D005819:Genetic Markers; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase