title: Analysis of a GT microsatellite in the promoter of the foxp3/scurfin gene in autoimmune diseases.
abstract: The aim of this study was to assess the possible association of the functional (GT)(n) microsatellite polymorphism in the FOXP3 gene with predisposition to several autoimmune diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), ulcerative colitis (UC), Crohn's disease, and celiac disease. We analyzed a case-control cohort composed of 231 SLE patients, 293 RA patients, 528 inflammatory bowel disease (354 Crohn's disease patients and 260 UC patients) patients, 103 celiac disease patients, and 274 healthy controls ethnically matched. Genotyping of (GT)(n) microsatellite was performed by polymerase chain reaction (PCR)-based method combined with fluorescent technology. We found no evidence for association of this polymorphism between controls and these autoimmune disease patients. Additionally, no differences in the genotype and allele distribution were found when patients were stratified according to clinical manifestation. The (GT)(n) microsatellite of the FOXP3 gene may not play a relevant role in the susceptibility to SLE, RA, inflammatory bowel disease, and celiac disease in our population.
authors.affiliation: Instituto de Parasitología y Biomedicina López Neyra, Granada, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Sánchez
authors.forename: Blanca
authors.initials: B
authors.lastname: Rueda
authors.forename: Gisela
authors.initials: G
authors.lastname: Orozco
authors.forename: Javier
authors.initials: J
authors.lastname: Oliver
authors.forename: Jose R
authors.initials: JR
authors.lastname: Vilchez
authors.forename: Laura
authors.initials: L
authors.lastname: Paco
authors.forename: Miguel A
authors.initials: MA
authors.lastname: López-Nevot
authors.forename: José L
authors.initials: JL
authors.lastname: Callejas
authors.forename: José M
authors.initials: JM
authors.lastname: Sabio
authors.forename: Maria
authors.initials: M
authors.lastname: Gómez-Garcia
authors.forename: A
authors.initials: A
authors.lastname: Nieto
authors.forename: Mario
authors.initials: M
authors.lastname: Delgado
authors.forename: Javier
authors.initials: J
authors.lastname: Martín
mesh_terms: D001172:Arthritis, Rheumatoid / Q000235:genetics; D001327:Autoimmune Diseases / Q000235:genetics*; D003424:Crohn Disease; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015212:Inflammatory Bowel Diseases; D008180:Lupus Erythematosus, Systemic / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats; D011401:Promoter Regions, Genetic
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors