Production of fruiting-body lectins of Pleurotus cornucopiae in methylotrophic yeast Pichia pastoris.
Fruiting-body lectin genes obtained from Pleurotus cornucopiae were expressed in Pichia pastoris Because of glycosylation of the products, their molecular mass was larger than that of the corresponding native lectins. They showed binding activity to porcine stomach mucin in the enzyme-linked lectin assay system, but did not agglutinate red blood cells.
Iijima
Naoto
N
Department of Bioresources Chemisoy Faculty of Horticulture, Chiba University 648 Matsudo, Chiba 271-8510, Japan.
Amano
Koh
K
Ando
Akikazu
A
Nagata
Yoshiho
Y