title: Immobilization of active hydrogenases by encapsulation in polymeric porous gels.
abstract: Hydrogenases encapsulated in porous polymeric silica gels retain significant levels of hydrogen production activity when compared to hydrogenases in solution using reduced methyl viologen as an electron donor. Encapsulated hydrogenases remain active after storage at room temperature for longer than four weeks and are less sensitive to proteolytic digestion. Nanoscopic confinement of active hydrogenases in solids paves the way for their potential use in hydrogen producing catalytic materials applications.
authors.affiliation: Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA. TElgren@Hamilton.edu
authors.forename: Timothy E
authors.initials: TE
authors.lastname: Elgren
authors.forename: Oleg A
authors.initials: OA
authors.lastname: Zadvorny
authors.forename: Eric
authors.initials: E
authors.lastname: Brecht
authors.forename: Trevor
authors.initials: T
authors.lastname: Douglas
authors.forename: Nikolay A
authors.initials: NA
authors.lastname: Zorin
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Maroney
authors.forename: John W
authors.initials: JW
authors.lastname: Peters
mesh_terms: D002214:Capsules / Q000737:chemistry; D003013:Clostridium / Q000201:enzymology; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D006864:Hydrogenase / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013696:Temperature; D020616:Thiocapsa roseopersicina / Q000201:enzymology
chemical_list: D002214:Capsules; D004800:Enzymes, Immobilized; D005782:Gels; D011108:Polymers; D006864:Hydrogenase