title: Close correlation of streptococcal DNase B (sdaB) alleles with emm genotypes in Streptococcus pyogenes.
abstract: DNase B is a major nuclease and a possible virulence factor in Streptococcus pyogenes. The allelic diversity of streptococcal DNase B (sdaB) gene was investigated in 83 strains with 14 emm genotypes. Of the 15 alleles identified, 11 alleles carried only synonymous nucleotide substitutions. On the other hand, 4 alleles had a non-synonymous substitution other than synonymous substitutions, resulting in the substitution of a single amino acid. The distribution of each allele was generally emm genotype-specific. Only sdaB7 was found in both emm2 and emm4. The promoter region was highly conserved and DNase B protein was similarly expressed in all alleles.
authors.affiliation: Department of Microbiology, Aichi Prefectural Institute of Public Health, Nagoya, Japan. masakado_matsumoto@pref.aichi.lg.jp
authors.forename: Masakado
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Sakae
authors.forename: Shinnosuke
authors.initials: S
authors.lastname: Hashikawa
authors.forename: Keizo
authors.initials: K
authors.lastname: Torii
authors.forename: Tadao
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Horii
authors.forename: Miyoko
authors.initials: M
authors.lastname: Endo
authors.forename: Rumi
authors.initials: R
authors.lastname: Okuno
authors.forename: Shoko
authors.initials: S
authors.lastname: Murayama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hirasawa
authors.forename: Rieko
authors.initials: R
authors.lastname: Suzuki
authors.forename: Junko
authors.initials: J
authors.lastname: Isobe
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tanaka
authors.forename: Chihiro
authors.initials: C
authors.lastname: Katsukawa
authors.forename: Aki
authors.initials: A
authors.lastname: Tamaru
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tomita
authors.forename: Kikuyo
authors.initials: K
authors.lastname: Ogata
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Ikebe
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
authors.forename: Michio
authors.initials: M
authors.lastname: Ohta
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D003851:Deoxyribonucleases / Q000032:analysis / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D005838:Genotype; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013223:Statistics as Topic; D013297:Streptococcus pyogenes / Q000201:enzymology / Q000235:genetics*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; C014689:streptococcal M protein; D003851:Deoxyribonucleases; C020069:deoxyribonuclease B