Taxane production in suspension culture of Taxus x media var. Hicksii carried out in flasks and bioreactor.
Paclitaxel and 10-deacetylbaccatin III (10-DAB III) were produced in suspension cultures of Taxus x media var. Hicksii grown in shake-flasks and in a 7-l bioreactor reaching, in the bioreactor, 4.4 mg l(-1) (on day 14) and 37.5 mg l(-1) (on day 11). In shake-flasks the highest total content of paclitaxel and 10-DAB III was 7.3 mg l(-1) (on day 4) and 8.8 mg l(-1) (on day 18). Phenylalanine, at 0.05 mM, increased paclitaxel accumulation in cells cultivated in bioreactor and flasks 30-fold and 9-fold (from 0.02 mg l(-1) to 0.6 mg l(-1) and to 0.2 mg l(-1), respectively). The 10-DAB III content in cells from flasks was increased from 0.4 mg l(-1) to 1.6 mg l(-1).
Syklowska-Baranek
Katarzyna
K
Department of Biology and Pharmaceutical Botany, Medical University of Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland, kasiasb@farm.amwaw.edu.pl.
Furmanowa
Miroslawa
M
D019149:Bioreactors; D001952:Bridged-Ring Compounds / Q000378:metabolism*; D017239:Paclitaxel / Q000096:biosynthesis; D043823:Taxoids / Q000378:metabolism*; D020946:Taxus / Q000378:metabolism*
D001952:Bridged-Ring Compounds; D043823:Taxoids; C080625:taxane; C042213:10-deacetylbaccatine III; D017239:Paclitaxel