A general method to modify BACs to generate large recombinant DNA fragments.
Bacterial artificial chromosome (BAC) has the capacity to clone DNA fragments in excess of 300 kb. It also has the considerable advantages of stable propagation and ease of purification. These features make BAC suitable in genetic research, such as library construction, transgenic mice production, and gene targeting constructs. Homologous recombination in Escherichia coli, a process named recombineering, has made the modification of BACs easy and reliable. We report here a modified recombineering method that can efficiently mediate the fusion of large DNA fragments from two or more different BACs. With the introduction of kanamycin-resistant gene and proposed rare-cutting restriction endonuclease (RCRE) sites into two BACs, a 82.6-kb DNA fragment containing the inverted human alpha-globin genes (theta, alpha1, alpha2, and zeta) from BAC191K2 and the locus control region (LCR) of human beta-globin gene locus (from the BAC186D7) was reconstructed. This approach for combining different BAC DNA fragments should facilitate many kinds of genomic experiments.
Shen
Wei
W
Huang
Yue
Y
Tang
Yi
Y
Liu
De-Pei
DP
Liang
Chih-Chuan
CC
D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000096:biosynthesis* / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D018390:Gene Targeting; D005818:Genetic Engineering / Q000379:methods*; D005914:Globins / Q000235:genetics; D019901:Locus Control Region / Q000235:genetics; D020161:Physical Chromosome Mapping
D004274:DNA, Recombinant; D005914:Globins; D004262:DNA Restriction Enzymes