title: Production and properties of phytase and acid phosphatase from a sake koji mold, Aspergillus oryzae.
abstract: We identified three types of acid phosphatase (ACP-I, ACP-II, and ACP-III) produced by Aspergillus oryzae in a submerged culture using only phytic acid as the phosphorous substrate. The optimum pH for the activities of the three enzymes was in the range of 4.5 to 5.5. Analysis of the substrate specificities of these enzymes revealed that ACP-I and ACP-III were acid phosphatases, and ACP-II was a phytase. These enzymes were produced during different periods of mycelial growth: ACP-II was produced during the early phase of cultivation (around 24 h), and ACP-I was produced between 24 to 72 h. ACP-III was detected after the production of ACP-I and ACP-II had ceased. The release of phosphate from phytic acid was expected to be due to the cooperative hydrolysis of these enzymes.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan. fujitaj@miyagi.jst-plaza.jp
authors.forename: Jin
authors.initials: J
authors.lastname: Fujita
authors.forename: Yu-Ichi
authors.initials: Y
authors.lastname: Yamane
authors.forename: Hisashi
authors.initials: H
authors.lastname: Fukuda
authors.forename: Yasuzo
authors.initials: Y
authors.lastname: Kizaki
authors.forename: Saburo
authors.initials: S
authors.lastname: Wakabayashi
authors.forename: Seiko
authors.initials: S
authors.lastname: Shigeta
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ono