Characterization of vacuolar membrane proteins changed in rice root treated with gibberellin.
Rice vacuolar membrane proteins changed by gibberellin (GA) were analyzed using a proteome approach. Vacuolar membrane fractions were isolated using a discontinuous sucrose/sorbitol system and 10 proteins increased in vacuolar membrane of the root, treated with GA(3) as compared with control. Fructose-1,6-bisphosphate aldolase C-1 and vacuolar H(+)-ATPase (V-ATPase) increased in root vacuolar membrane by GA(3) interacted in rice roots. It suggests that aldolase C-1 regulates the V-ATPase mediated control of cell elongation that determines root growth.
Konishi
Hirosato
H
Department of Molecular Genetics, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Maeshima
Masayoshi
M
Komatsu
Setsuko
S
D000251:Adenosine Triphosphatases / Q000737:chemistry; D000595:Amino Acid Sequence; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005635:Fructosediphosphates / Q000737:chemistry; D005875:Gibberellins / Q000737:chemistry*; D047468:Immunoprecipitation; D007525:Isoelectric Focusing; D008954:Models, Biological; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D030821:Plants, Genetically Modified / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D016622:Silver Staining; D013012:Sorbitol / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013395:Sucrose / Q000737:chemistry; D013696:Temperature; D025262:Vacuolar Proton-Translocating ATPases / Q000737:chemistry; D014617:Vacuoles / Q000737:chemistry*
D004791:Enzyme Inhibitors; D005635:Fructosediphosphates; D005875:Gibberellins; D010455:Peptides; D010940:Plant Proteins; D013012:Sorbitol; D013395:Sucrose; D000251:Adenosine Triphosphatases; D025262:Vacuolar Proton-Translocating ATPases; C029063:fructose-1,6-diphosphate