Pentylenetetrazole kindling induces neuronal cyclin B1 expression in rat hippocampus.
The purpose of the study was to explore the involvement of cell cycle events in the neuronal death induced by repeated seizures. Pentylenetetrazole (PTZ) kindling was used as a model of seizure-induced hippocampal neurodegeneration. Immunohistochemical approach was applied to detect cell cycle markers (cyclins and cycline-dependent kinases) in hippocampus. PTZ-kindling in rats induced moderate neuronal cell loss in hippocampal fields CA1, CA 3, CA 4, and dentate gyrus. The majority of damaged cells in hippocampi of PTZ-kindled rats were cycline B1 positive, while no expression of either other cell cycle markers or TUNEL-positive (apoptotic) nuclei could be revealed. Since cycline B1 expression has been described in hippocampal neurons of patients with temporal lobe epilepsy by [Z. Nagy, M.M. Esiri, Neuronal cyclin expression in the hippocampus in temporal lobe epilepsy, Exp. Neurol. 150 (1998) 240-247], it is suggested that PTZ-kindling may be a suitable model to study the mechanisms of seizure-induced neuronal death.
Pavlova
Tatyana
T
Department of Functional Biochemistry of the Nervous System, Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 5a Butlerov Str., Moscow 117485, Russia.
Stepanichev
Mikhail
M
Gulyaeva
Natalia
N
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002452:Cell Count / Q000379:methods; D016923:Cell Death / Q000187:drug effects; D019926:Cyclin B / Q000378:metabolism*; D056744:Cyclin B1; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry / Q000379:methods; D020287:In Situ Nick-End Labeling / Q000379:methods; D007696:Kindling, Neurologic / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000187:drug effects*; D010433:Pentylenetetrazole; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods
C534701:Ccnb1 protein, rat; D019926:Cyclin B; D056744:Cyclin B1; D010433:Pentylenetetrazole