A Brownian dynamics study of the effects of cytochrome f structure and deletion of its small domain in interactions with cytochrome c6 and plastocyanin in Chlamydomonas reinhardtii.
The availability of seven different structures of cytochrome f (cyt f) from Chlamydomonas reinhardtii allowed us, using Brownian dynamics simulations, to model interactions between these molecules and their redox partners, plastocyanin (PC) and cytochrome c6 (cyt c6) in the same species to study the effect of cyt f structure on its function. Our results showed that different cyt f structures, which are very similar, produced different reaction rates in interactions with PC and cyt c6. We were able to attribute this to structural differences among these molecules, particularly to a small flexible loop between A-184 and G-191 (which has some of the highest crystallographic temperature factors in all of the cyt f structures) on the cyt f small domain. We also showed that deletion of the cyt f small domain affected cyt c6 more than PC, due to their different binding positions on cyt f. One function of the small domain in cyt f may be to guide PC or cyt c6 to a uniform dock with cyt f, especially due to electrostatic interactions with K-188 and K-189 on this domain. Our results could serve as a good guide for future experimental work on these proteins to understand better the electron transfer process between them. Also, these results demonstrated the sensitivity and the power of the Brownian dynamics simulations in the study of molecular interactions.
Haddadian
Esmael J
EJ
Biophysics Program and Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA.
Gross
Elizabeth L
EL
D000465:Algorithms; D000818:Animals; D001703:Biophysics / Q000379:methods*; D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D045365:Cytochromes c6 / Q000235:genetics*; D045348:Cytochromes f / Q000737:chemistry* / Q000235:genetics*; D030562:Databases, Protein; D017353:Gene Deletion; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D010970:Plastocyanin / Q000235:genetics*; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D055672:Static Electricity; D013696:Temperature; D013997:Time Factors
D045365:Cytochromes c6; D010970:Plastocyanin; D045348:Cytochromes f