title: Tepidimonas taiwanensis sp. nov., a novel alkaline-protease-producing bacterium isolated from a hot spring.
abstract: The bacterial strain designated I1-1(T) was isolated from a hot spring located in the Pingtung area, southern Taiwan. Cells of this organism were Gram reaction negative rods, motile by a single polar flagellum. Optimum conditions for growth were 55 degrees C and pH 7. Strain I1-1(T) grew well in lower nutrient media such as 5-10% Luria-Bertani broth, and its extracellular products expressed alkaline protease activity. The 16S rRNA gene sequence analysis indicates that strain I1-1(T) is a member of beta-Proteobacteria. On the basis of a phylogenetic analysis of 16S rDNA sequences, DNA-DNA similarity data, whole-cell protein analysis, physiological and biochemical characteristics, as well as fatty acid compositions, the organism belonged to the genus Tepidimonas and represented a novel species within this genus. The predominant cellular fatty acids of strain I1-1(T) were 16:0 (about 41%), 18:1 omega7c (about 13%), and summed feature 3 [16:1 omega7c or 15:0 iso 2OH or both (about 26%)]. Its DNA base ratio was 68.1 mol%. We propose to classify strain I1-1(T) (=BCRC 17406(T)=LMG 22826(T)) as Tepidimonas taiwanensis sp. nov.
authors.affiliation: Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Tien-Lai
authors.initials: TL
authors.lastname: Chen
authors.forename: Yi-Ju
authors.initials: YJ
authors.lastname: Chou
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.forename: Bhagwath
authors.initials: B
authors.lastname: Arun
authors.forename: Chiu-Chung
authors.initials: CC
authors.lastname: Young
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification; D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology; D005798:Genes, Bacterial; D006358:Hot Temperature; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012697:Serine Endopeptidases / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012697:Serine Endopeptidases; C032923:microbial serine proteinases