Effect of IPs, cAMP, and cGMP on the hPL and hCG secretion from human term placenta.
The acute control of human placental lactogen (hPL) and chorionic gonadotrophin (hCG) secretion by the placenta remains elusive. The in vitro release of both hormones can be stimulated by calcium inflow and by albumin. To investigate the placental secretory response to putative ligand(s) present in the maternal circulation, we evaluated the coupling of the hPL and hCG releases from term placenta with intracellular signaling pathways. Addition of NaF, forskolin or sodium nitroprusside, activators of the inositol phosphates (IPs), cAMP and cGMP pathways, significantly increased their respective messengers in villous explants but failed to affect the hPL and hCG releases from syncytiotrophoblast. By contrast, albumin did not modify the IPs, cAMP and cGMP villous content but significantly stimulated the placental hormonal release. These data suggest that the hPL and hCG secretion is not regulated through the IPs, cAMP and cGMP signaling pathways.
Lambot
Nathalie
N
Laboratory of Experimental Hormonology, Université Libre de Bruxelles, 808 Lennik Road, Campus Erasme, CP 626, Brussels 1070, Belgium.
Nathalie
Lambot
L
Lebrun
Philippe
P
Philippe
Lebrun
L
Delporte
Christine
C
Christine
Delporte
D
De Vriese
Carine
C
Carine
De Vriese
de V
Delogne-Desnoeck
Josiane
J
Josiane
Delogne-Desnoeck
DD
Vanbellinghen
Anne Marie
AM
Anne Marie
Vanbellinghen
V
Graff
Guy
G
Guy
Graff
G
Meuris
Sylvain
S
Sylvain
Meuris
M
D006063:Chorionic Gonadotropin / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D006152:Cyclic GMP / Q000502:physiology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000502:physiology*; D009599:Nitroprusside / Q000494:pharmacology; D010920:Placenta / Q000187:drug effects / Q000502:physiology*; D010928:Placental Lactogen / Q000378:metabolism*; D011247:Pregnancy; D015398:Signal Transduction; D012969:Sodium Fluoride / Q000494:pharmacology
D006063:Chorionic Gonadotropin; D007295:Inositol Phosphates; D009599:Nitroprusside; D005576:Colforsin; D012969:Sodium Fluoride; D010928:Placental Lactogen; D000242:Cyclic AMP; D006152:Cyclic GMP