Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene.
An extended range whole-cell tetracycline biosensor strain was constructed by insertion of the tet(M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid pTGFP2, which contains a transcriptional fusion between a tetracycline regulated promoter and the green fluorescent protein gene. Tetracycline, oxytetracycline, chlortetracycline and minocycline all effectively induced the resulting Escherichia coli MC4100/pTGM biosensor and similar dose-response characteristics were recorded by flow cytometry for all four compounds. The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ngml(-1) to 16 microgml(-1), which represents a significant improvement of the original version.
Bahl
Martin Iain
MI
Department of Microbiology, University of Copenhagen, Sølvgade 83H, 1307 Copenhagen K, Denmark.
Hansen
Lars Hestbjerg
LH
Sørensen
Søren J
SJ
D001426:Bacterial Proteins / Q000235:genetics*; D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards; D002751:Chlortetracycline / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D005434:Flow Cytometry; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000494:pharmacology; D010118:Oxytetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics*; D013754:Tetracyclines / Q000494:pharmacology*
D001426:Bacterial Proteins; C083138:Tet M resistance protein, Bacteria; D013754:Tetracyclines; D008911:Minocycline; D002751:Chlortetracycline; D010118:Oxytetracycline