title: Determination and locational variations in the quantity of hydroxyanthraquinones and their glycosides in rhizomes of Rheum emodi using high-performance liquid chromatography.
abstract: Locational variations in the quantity of five hydroxyanthraquinone derivatives (emodin glycoside (1), chrysophanol glycoside (2), emodin (3), chrysophanol (4) and physcion (5)) in the rhizomes of Rheum emodi are described. A simple and reliable method was developed for quantitation of compounds (1-5) in the methanolic extract of rhizomes of R. emodi using reverse-phase high-performance liquid chromatography (HPLC) with photo-diode array detector (PDA). The separation was carried out using a Purospher((R))-Star RP-18 e column (4.6mm i.d.x 250 mm, 5 microm) under the following conditions: acetonitrile:methanol (95:5, v/v) (solvent A) and water:acetic acid (99.9:0.1, v/v) (solvent B) as mobile phase with a linear gradient elution at a flow rate of 0.8 mL/min. The detection wavelength was set at 290 nm. Regression equation revealed a linear relationship (r(2)>0.9901) between the mass of hydroxyanthraquinone derivatives injected and the peak areas. The detection limits (S/N=3) ranged from 0.56 to 3.50 ng/mL and the recoveries ranged from 95.7 to 103.5% for five hydroxyanthraquinone derivatives. Compound 2 was found in maximum quantity (up to 2.23%) in the rhizomes from all the three locations (L(1), L(2) and L(3)) while compound 5 was found in the least quantity (up to 0.19%).
authors.affiliation: Natural Plant Products Division, Institute of Himalayan Bioresource Technology (CSIR), Post Box No. 6, Palampur, Himachal Pradesh 176061, India.
authors.forename: Subash C
authors.initials: SC
authors.lastname: Verma
authors.forename: Narendra P
authors.initials: NP
authors.lastname: Singh
authors.forename: Arun K
authors.initials: AK
authors.lastname: Sinha
mesh_terms: D000880:Anthraquinones / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D015203:Reproducibility of Results; D012250:Rheum / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D006027:Glycosides; C008905:physcione; D004642:Emodin; C027113:chrysophanic acid