title: Upregulating of Fas, integrin beta4 and P53 and depressing of PC-PLC activity and ROS level in VEC apoptosis by safrole oxide.
abstract: Previously, we found that safrole oxide could trigger vascular endothelial cell (VEC) apoptosis. In this study, to investigate its mechanism to induce apoptosis in VECs, the activities of nitric oxide synthetase and phosphatidylcholine specific phospholipase C, the level of reactive oxygen species and the expressions of Fas, integrin beta4 and P53 were analyzed. The data showed that safrole oxide induced apoptosis by increasing the expressions of Fas, integrin beta4 and P53, and depressing the activity of Ca(2+)-independent phosphatidylcholine-specific phospholipase C and intracellular reactive oxygen species levels in VECs.
authors.affiliation: Institute of Developmental Biology, School of Life Science, Shandong University, Jinan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Junying
authors.initials: J
authors.lastname: Miao
authors.forename: Baoxiang
authors.initials: B
authors.lastname: Zhao
authors.forename: Shangli
authors.initials: S
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D015536:Down-Regulation; D042783:Endothelial Cells / Q000737:chemistry; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D039663:Integrin beta4 / Q000032:analysis / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012451:Safrole / Q000031:analogs & derivatives* / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism; D015854:Up-Regulation; D019014:fas Receptor / Q000378:metabolism
chemical_list: D039663:Integrin beta4; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor; C009196:safrole oxide; D019001:Nitric Oxide Synthase; D010738:Type C Phospholipases; C105598:phosphatidylcholine-specific phospholipase C; D012451:Safrole