Biphasic interaction of gamma-globulin with copper cations.
Interactions of human serum gamma-globulin with copper cations in solution were studied by differential ultraviolet spectrophotometry. Copper in supraphysiological concentrations increases optical density of protein solution, reflecting the effect of gamma-globulin saturation with metal. In physiological and lower concentrations of copper cations we observed hypochromia in the protein absorption spectrum. Conformational changes in g-globulin molecule during interactions with copper by the surface and intramolecular binding sites and possible role of bivalent metal cations in the maintenance of certain conformations of immunoactive serum proteins are discussed.
Cheknev
S B
SB
Laboratory of Cell-Cell Interactions, NF Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow. cheknev@riem.ru
Babaeva
E E
EE
Zharkova
M S
MS
D002412:Cations / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D006801:Humans; D011487:Protein Conformation; D013053:Spectrophotometry; D005719:gamma-Globulins / Q000737:chemistry*
D002412:Cations; D005719:gamma-Globulins; D003300:Copper