title: Monkey pulpal response to an MMA-based resin cement as adhesive luting for indirect restorations.
abstract: OBJECTIVE
  The objective of this study was to evaluate the pulpal response to a newly-developed MMA resin cement (MultiBond, Tokuyama) when used for adhesively luting composite resin inlays.
  
  
  METHODS
  Cervical cavities were prepared in monkey teeth. The teeth were randomly divided into 3 groups. In the experimental group, a self-etching primer and a resin cement were applied to the cavities, and then hybrid composite inlays (Estenia, Kuraray) were inserted using freshly mixed resin cement. In the other groups, a zinc oxide/eugenol cement (Eugedain, Showa Yakuhin Kakou) or a glass-ionomer cement (Fuji II, GC) was used to fill the cavity. The teeth were then extracted after 3, 30, and 90 days, fixed in 10% buffered formalin solution, and prepared using routine histological techniques. Five-mum-thick sections were stained with hematoxylin and eosin, or Brown & Brenn gram stain for bacterial observation. Histopathological reactions in the pulp tissue and bacterial penetration along the cavity walls were assessed using a standardized score.
  
  
  RESULTS
  No serious inflammatory reactions in the pulp, such as necrosis or abscess formation, were observed in any of the experimental periods, except for 1 case after 30 days, in which a pulpal exposure was suspected. Disarrangement of the odontoblast layer and deposition of reparative dentin were the major reactions observed in this specimen. No bacterial penetration along the cavity walls was detected. The monkey pulpal response and in vivo sealing ability of the MMA resin cement in combination with the self-etching primer was considered as good as that of the glass-ionomer cement.
  
  
  CONCLUSIONS
  The new MMA resin cement showed acceptable biological compatibility to the monkey pulp when used to adhesively lute composite resin inlays.
authors.affiliation: Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. shimada.ope@tmd.ac.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shimada
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Seki
authors.forename: Mohammed Akhtar
authors.initials: MA
authors.lastname: Uzzaman
authors.forename: Vanthana
authors.initials: V
authors.lastname: Sattabanasuk
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Sasafuchi
authors.forename: Richard M
authors.initials: RM
authors.lastname: Foxton
authors.forename: Masayuki
authors.initials: M
authors.lastname: Otsuki
authors.forename: Junji
authors.initials: J
authors.lastname: Tagami
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D003188:Composite Resins; D003738:Dental Cements / Q000494:pharmacology*; D003782:Dental Pulp / Q000187:drug effects*; D005899:Glass Ionomer Cements / Q000494:pharmacology; D000882:Haplorhini; D008768:Methylmethacrylates / Q000494:pharmacology*; D011140:Polyurethanes; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: D000180:Acrylic Resins; C029012:Composite Dental Resin; D003188:Composite Resins; D003738:Dental Cements; D005899:Glass Ionomer Cements; D008768:Methylmethacrylates; C482145:Multibond; D011140:Polyurethanes; D015035:Zinc Oxide-Eugenol Cement; C060877:Dakril 4B