A novel single-nucleotide substitution, Glu 4 Lys, in the leukotriene C4 synthase gene associated with allergic diseases.
Cysteinyl leukotrienes (cysLTs) play important roles in bronchial asthma, and can mediate bronchial smooth muscle constriction and increase mucous secretion, vascular permeability and cellular infiltration. We identified a novel heterozygous single-nucleotide substitution 10G>A (Glu 4 Lys) in the first exon of the leukotriene C4 synthase gene (LTC4S). This substitution was detected in 5 of 141 allergic patients, but not in 110 nonallergic subjects. There was a difference in the Glu 4 Lys frequency between the allergic patients and nonallergic subjects (Fisher's exact test, p=0.0460). The five patients with Glu 4 Lys had allergic diseases such as bronchial asthma and/or allergic dermatitis. Furthermore, a familial analysis of Glu 4 Lys revealed a link with allergic diseases. Thus, our results suggest that Glu 4 Lys in the LTC4S might be associated with allergic diseases.
Yoshikawa
Kaori
K
Department of Pediatrics, Graduate School of Medicine, Gifu University, Yoro-gun, Gifu 501-1193, Japan. kimatan@tj8.so-net.ne.jp
Matsui
Eiko
E
Kaneko
Hideo
H
Fukao
Toshiyuki
T
Inoue
Ryosuke
R
Teramoto
Takahide
T
Shinoda
Shinji
S
Fukutomi
Osamu
O
Aoki
Minako
M
Kasahara
Kimiko
K
Kondo
Naomi
N
D000293:Adolescent; D000328:Adult; D019943:Amino Acid Substitution / Q000235:genetics; D001249:Asthma / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000235:genetics*; D005260:Female; D018698:Glutamic Acid / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000235:genetics*; D007223:Infant; D008239:Lysine / Q000235:genetics; D008297:Male; D017354:Point Mutation; D012130:Respiratory Hypersensitivity / Q000235:genetics*
D018698:Glutamic Acid; D005982:Glutathione Transferase; C041116:leukotriene-C4 synthase; D008239:Lysine