Validation of an HPLC method for quantitation of MDMA in tablets.
An isocratic reversed-phase high-performance liquid chromatography (HPLC) method is developed and validated for the quantitation of 3,4-methylenedioxymethamphetamine (MDMA) in tablets. The chromatographic separation is achieved with potassium phosphate buffer (pH 3.2)-acetonitrile (9:1, v/v) as mobile phase, a Chromspher B column, and UV detection at 210 nm. The calibration curve is linear from 1.4 to 111 microg/mL. The percent relative standard deviation for intra- and interday precision studies is 2.7% each. The measurement uncertainty is estimated to 9%. The method is specific and successfully used for routine quantitation of MDMA in tablets.
Müller
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Department of Forensic Chemistry, University of Copenhagen, Frederik V's Vej 11, 2100 Copenhagen, Denmark. Irene.breum@forensic.ku.dk
Windberg
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CN
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006213:Hallucinogens / Q000737:chemistry*; D018817:N-Methyl-3,4-methylenedioxyamphetamine / Q000032:analysis*; D012680:Sensitivity and Specificity; D013607:Tablets / Q000737:chemistry*
D006213:Hallucinogens; D013607:Tablets; D018817:N-Methyl-3,4-methylenedioxyamphetamine