In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecule PCR and cell-free protein synthesis.
A mutant library of Burkholderia cepacia lipase KWI-56 was constructed on microplates by a cell-free process and tested with a chromogenic assay. This high-throughput construction system can be used to screen mutant proteins based on their catalytic activity.
Koga
Yuichi
Y
Laboratory of Molecular Biotechnology, Graduate School of Biological & Agricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Kobayashi
Kouei
K
Yang
Junhao
J
Nakano
Hideo
H
Yamane
Tsuneo
T