title: [Genomic analysis of serine carboxypeptidase-like protein family of Arabidopsis thaliana].
abstract: Based on hidden Markov models (HMM), the paper utilized reported SCP (Serine Carboxypeptidases) protein sequences as datasets to build HMM profile. To search Arabidopsis thaliana whole proteome,and identified 54 SCPL (Serine Carboxypeptidase-Like) proteins coding genes. The intron-exon structure, the chromosome mapping and the characteristic of coded protein sequences of those 54 putative genes were analyzed in details, revealing seven gene clusters probably resulted from recent gene duplication. This implied that a remarkable number of Arabidopsis thaliana SCPL genes are harboring alternative splice sites. Phylogenetics evolution analysis suggested 88.9% proteins encoded by Arabidopsis genes belong to two string subfamily of carboxypeptidase, I or II, while only 11.1% proteins fall into single string carboxypeptidase III subfamily. Our results indicated besides the facts that all enzymes of this family contained a central catalytic domain of unique topology and three dimensional structure designated as "alpha/beta hydrolase fold", the DNA and their encoded protein sequences also gave clues to phylogentics studies.
authors.affiliation: Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.forename: Qing-Po
authors.initials: QP
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Jia
authors.forename: Qing-Zhong
authors.initials: QZ
authors.lastname: Xue
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics*; D002268:Carboxypeptidases / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D019143:Evolution, Molecular; D023281:Genomics / Q000379:methods; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D002268:Carboxypeptidases; C022633:serine carboxypeptidase