title: A core promoter element downstream of the TATA box that is recognized by TFIIB.
abstract: We have defined a core promoter element downstream of the TATA box that is recognized by TFIIB. This involves a DNA-binding domain in TFIIB that is distinct from the helix-turn-helix motif (which recognizes an element upstream of the TATA box). The TFIIB recognition element we describe regulates transcription in a manner that is promoter context-dependent, particularly with respect to the TFIIB recognition element that is located upstream of the TATA box. Thus TFIIB can recognize two distinct sequence elements that flank the TATA box, employing independent DNA-binding motifs and cooperating in the regulation of transcription.
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Deng
authors.forename: Stefan G E
authors.initials: SG
authors.lastname: Roberts
mesh_terms: D000256:Adenoviridae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002474:Cell-Free System / Q000737:chemistry / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020218:Response Elements / Q000235:genetics; D016385:TATA Box / Q000235:genetics; D035581:Transcription Factor TFIIB / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D035581:Transcription Factor TFIIB