title: [Effect of high glucose on the expression of fibronectin and plasminogen activator inhibitor-1 in human peritoneal mesothelial cells].
abstract: OBJECTIVE
  To evaluate the effect of high glucose on the expression of fibronectin (FN) and plasminogen activator inhibitor-1 (PAI-1) in human peritoneal mesothelial cells (HPMC).
  
  
  METHODS
  The cultured HPMC were exposed to the culture medium containing different concentrations of glucose (1.5%, 2.5%, 4.25%) for 6, 12, 24, 48 hours, and normal culture medium was used as control. FN and PAI-1 in supernatant were detected by enzyme-linked immunoassay(ELISA).
  
  
  RESULTS
  The glucose at 1. 5%, 2.5%, 4.25% resulted in a time- and concentration-dependent increase in FN expression in HPMC(P < 0.05). The glucose at 2.5%, 4.25% increased PAI-1 expression in HPMC (P < 0.05), and the effect was time- and concentration-dependent. PAI-1 expression was not affected by 1.5% glucose (P > 0.05).
  
  
  CONCLUSIONS
  High glucose increases the expression of FN and PAI-1 in HPMC, thus playing a part in the progression of peritoneal fibrosis.
authors.affiliation: Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ji
authors.forename: Bin
authors.initials: B
authors.lastname: Zhuang
authors.forename: Li-Chuan
authors.initials: LC
authors.lastname: Yang
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Liu
authors.forename: Xian-Rong
authors.initials: XR
authors.lastname: Liu
mesh_terms: D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D010530:Peritoneal Dialysis / Q000009:adverse effects; D010537:Peritoneum / Q000166:cytology* / Q000378:metabolism; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis*
chemical_list: D005353:Fibronectins; D017395:Plasminogen Activator Inhibitor 1; D005947:Glucose