The nucleolar structure and the activity of NopA100, a nucleolin-like protein, during the cell cycle in proliferating plant cells.
For the purpose of gaining knowledge of the relationships between cell proliferation and ribosome biogenesis, as two fundamental mutually interconnected cellular processes, studies were performed on cell populations synchronized in their cell-cycle progression by treatment with hydroxyurea, followed by sampling at different times after its removal. A structural rearrangement of the nucleolus was observed throughout the interphase, along with changes in the relative amounts of different nucleolar subcomponents. A structural model of nucleolar organization was associated with each interphase period. Throughout interphase, the nucleolin-like protein, NopA100, was immunodetected in the dense fibrillar component of the nucleolus, preferentially near fibrillar centers and its levels were shown to increase from G1 to G2. A western blotting analysis of soluble nuclear protein extracts with anti-NopA100 antibody resulted in the intense labeling of a 100-kDa band, but also of a series of proteins related to it, suggesting that NopA100 undergoes a physiological process of proteolytic maturation, similar to that described for mammalian nucleolin, but not reported in other biological model systems. Physiological proteolysis of NopA100, related to cell-cycle progression, was confirmed after the nuclei extracted from synchronized cells were treated with the protease inhibitor, leupeptin, which resulted in an increase of the 100-kDa band at the expenses of the decrease of some other bands, according to the cell-cycle stages. We therefore conclude that there is a relationship between the increase in nucleolar activity, cell-cycle progression, nucleolar structure, the activity of NopA100, and the proteolysis of this nucleolin-like protein.
González-Camacho
Fernando
F
Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28040, Madrid, Spain.
Medina
Francisco Javier
FJ
D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology; D002466:Cell Nucleolus / Q000502:physiology* / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000187:drug effects; D049109:Cell Proliferation; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D016193:G1 Phase / Q000502:physiology; D016195:G2 Phase / Q000502:physiology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008938:Mitosis / Q000187:drug effects; D019697:Onions / Q000166:cytology*; D010750:Phosphoproteins / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D000097796:Nucleolin
D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin