title: Proteome analysis for the identification of in vivo estrogen-regulated proteins in bone.
abstract: Estrogen deficiency results in a reduced bone mass, which can be prevented by treatment with estrogens. This study used a proteomic approach for the first time to obtain a global perspective of estrogens' effects on whole-bone proteins. Bone proteome profiles were examined in three groups of mice: (1) sham-operated with normal ovarian functions, (2) ovariectomised and (3) ovariectomised with estrogen replacement therapy. Bone proteins extracted from the humerus were separated by 2-DE and visualised by CBB colloidal staining. Spot detection and quantification was done by image analysis. Differentially expressed proteins were identified by MS and database search, using peptide mass fingerprint and peptide sequence analysis. Differential expression analysis in the three experimental groups showed significant changes for 14 proteins. These included proteins related to bone metabolism, cytoskeleton components and energy metabolic pathways. Our data suggest that some proteins related to cytoskeleton and to energy pathways, such as tropomyosins, aconitase 2 and enolase beta, might be new molecular targets responsive to the effects of estrogen. Differentially expressed proteins identified in this model may offer a useful starting point for elucidating novel aspects of the pleiotropic effects of estrogens on bone.
authors.affiliation: Department of Environmental Health Sciences, Istituto di Ricerche Farmacologiche Mario Negri, Via Eritrea 62, 20157 Milan, Italy. rpastorelli@marionegri.it
authors.forename: Roberta
authors.initials: R
authors.lastname: Pastorelli
authors.forename: Donatella
authors.initials: D
authors.lastname: Carpi
authors.forename: Luisa
authors.initials: L
authors.lastname: Airoldi
authors.forename: Chiara
authors.initials: C
authors.lastname: Chiabrando
authors.forename: Renzo
authors.initials: R
authors.lastname: Bagnati
authors.forename: Roberto
authors.initials: R
authors.lastname: Fanelli
authors.forename: Sofia
authors.initials: S
authors.lastname: Moverare
authors.forename: Claes
authors.initials: C
authors.lastname: Ohlsson
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006811:Humerus / Q000187:drug effects / Q000378:metabolism; D013058:Mass Spectrometry; D051379:Mice; D010052:Ovariectomy; D011506:Proteins / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004967:Estrogens; D011506:Proteins; D020543:Proteome