Comparison of renal damage by iodinated contrast or gadolinium in an acute renal failure rat model based on serum creatinine levels and apoptosis degree.
This study was undertaken to compare renal damage, as determined by serum creatinine and degree of apoptosis, caused by iodinated contrast or gadolinium in an acute renal failure (ARF) rat model. Rats were divided into three groups; controls (n=3), a CT contrast medium group (n=9), and an MR contrast medium group (n=9). The CT and MR groups were further subdivided into three groups, namely, low, standard, and high dose subgroups. Renal function was evaluated by determining serum creatinine levels; before ARF, and 48 hr after ARF and contrast administration. Apoptosis was assayed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). No significant creatinine level differences were observed between the CT and MR groups (p=0.116). Degrees of apoptosis in the renal cortex and medulla were more severe in the CT contrast medium group than in the control or MR contrast medium group (p<0.05). The study shows that CT contrast medium did not aggravate renal function more so than MR contrast medium in this ARF rat model. However, apoptosis examination in the renal cortex and medulla indicated that CT contrast medium induced more severe apoptosis than MR contrast medium (p<0.05). We conclude that CT contrast medium can be used for renal imaging studies when subjects are well hydrated and preventive medication is administered.
Kwak
Hyo-Sung
HS
Department of Radiology, Chonbuk National University Medical School, Chonju, Korea.
Lee
Young-Hwan
YH
Han
Young-Min
YM
Jin
Gong-Yong
GY
Kim
Won
W
Chung
Gyung-Ho
GH
D058186:Acute Kidney Injury / Q000097:blood* / Q000000981:diagnostic imaging / Q000473:pathology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D003287:Contrast Media / Q000009:adverse effects; D003401:Creatine / Q000097:blood*; D005260:Female; D019786:Gadolinium DTPA / Q000009:adverse effects*; D007472:Iohexol / Q000009:adverse effects / Q000031:analogs & derivatives*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology*; D008297:Male; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley
D003287:Contrast Media; D007472:Iohexol; C038192:iopromide; D019786:Gadolinium DTPA; D003401:Creatine