title: Immobilization of enzymes on a microchannel surface through cross-linking polymerization.
abstract: A novel and facile method for the preparation of an enzyme-immobilized microreactor has been developed in which enzymes are immobilized as an enzyme-polymer membrane formed on the inner wall of the microchannel by a cross-linking polymerization method; the resulting microreactor shows excellent reaction performance and stability against denaturating agents.
authors.affiliation: Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 807-1 Shuku, Tosu, Saga 841-0052, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Honda
authors.forename: Masaya
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Maeda
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002918:Chymotrypsin / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004151:Dipeptides / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D003432:Cross-Linking Reagents; D004151:Dipeptides; D004800:Enzymes, Immobilized; C007866:glutarylphenylalanine-4-nitroanilide; D011138:Polytetrafluoroethylene; D002918:Chymotrypsin