Molecular cloning and phylogenetic analysis of inflammatory cytokines of Camelidae (llama and camel).
We cloned, sequenced and analyzed the cDNAs encoding Camelidae inflammatory cytokines, including llama (lama glama) interleukin (IL)-1alpha, IL-1beta, IL-6, tumor necrosis factor (TNF)-alpha and camel (Camelus bactrianus) IL-6 and TNF-alpha. The similarity levels of the deduced amino acid sequences of IL-1alpha, IL-1beta, IL-6 and TNF-alpha from llama (camel) to those from other mammalian species, ranged from 60.7% to 87.7%, 52.8% to 75.3%, 41.4% to 98.6%, and 72.9% to 99.6%, respectively. Phylogenetic analyses based on nucleic acid sequences showed that llama IL-1alpha, IL-1beta, IL-6 and TNF-alpha were more closely related to those of camel, pig, cattle, sheep and horse than to those of human, dog, cat, mouse and rat.
Odbileg
Raadan
R
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Japan.
Konnai
Satoru
S
Ohashi
Kazuhiko
K
Onuma
Misao
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002161:Camelids, New World / Q000235:genetics*; D002162:Camelus / Q000235:genetics*; D003001:Cloning, Molecular; D016000:Cluster Analysis; D016207:Cytokines / Q000235:genetics*; D017931:DNA Primers; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D016207:Cytokines; D017931:DNA Primers