title: Pneumothorax-associated pleural eosinophilia in mice is interleukin-5 but not interleukin-13 dependent.
abstract: OBJECTIVE
  To establish a murine model of pneumothorax-associated pleural eosinophilia and to examine the role of interleukin (IL)-5 and IL-13 in the pathogenesis of this reaction.
  
  
  METHODS
  An animal study.
  
  
  METHODS
  One hundred thirty-seven C57/Bl-6 mice were used in this study. Wild-type animals were injected intrapleurally with 0.4 mL of air and were killed at different time points (30 min to 7 days) after the injection. IL-5 knockout and IL-13 knockout animals were killed 24 h and 48 h after the injection. Pleural inflammation was assessed by pleural lavage (PL).
  
  
  RESULTS
  PL cells were significantly increased following the induction of pneumothorax. The peak number of neutrophils, observed at 12 h, was 900 times higher than the control. The peak number of eosinophils, observed at 48 h, was 700 times higher than the control. Lymphocytes and mononuclear cells increased threefold and fourfold, respectively. IL-5 knockout mice had significantly less PL eosinophils than that the wild-type or the IL-13 knockout mice at 24 h (150 +/- 46/microL, 903 +/- 244/microL, and 912 +/- 168/microL, respectively; p = 0.013) and 48 h (181 +/- 45/microL, 1,587 +/- 212/microL, and 1,379 +/- 364/microL, respectively; p = 0.003).
  
  
  CONCLUSIONS
  Pneumothorax induces an inflammatory reaction of the mouse pleura, mainly characterized by increased neutrophils and eosinophils. IL-5 but not IL-13 is required for pneumothorax-associated pleural eosinophilia.
authors.affiliation: Marianthi Simou Laboratory, Department of Critical Care and Pulmonary Services, Athens Medical School, Evangelismos Hospital, 45-47 Ipsilandou Str., 10675 Athens, Greece. jkalomenidis@hotmail.com
authors.forename: Ioannis
authors.initials: I
authors.lastname: Kalomenidis
authors.forename: Yubiao
authors.initials: Y
authors.lastname: Guo
authors.forename: R Stokes
authors.initials: RS
authors.lastname: Peebles
authors.forename: Kirk B
authors.initials: KB
authors.lastname: Lane
authors.forename: Spyros
authors.initials: S
authors.lastname: Papiris
authors.forename: Jack
authors.initials: J
authors.lastname: Elias
authors.forename: Richard W
authors.initials: RW
authors.lastname: Light
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007249:Inflammation / Q000276:immunology; D018793:Interleukin-13 / Q000276:immunology*; D015848:Interleukin-5 / Q000172:deficiency / Q000276:immunology*; D007958:Leukocyte Count; D018655:Lymphocyte Count; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011030:Pneumothorax / Q000209:etiology* / Q000276:immunology; D011657:Pulmonary Eosinophilia / Q000150:complications / Q000276:immunology*
chemical_list: D018793:Interleukin-13; D015848:Interleukin-5