The effect of low dose gamma irradiation on the differentiation and maturation of monocyte derived dendritic cells.
The effects of gamma-irradiation on the differentiation of peripheral blood monocytes (PBM) into monocyte derived dendritic cells (MDC), their maturation, and subsequent ability to present antigen to T cells was studied. Undifferentiated MDC were more sensitive to gamma-irradiation induced apoptosis than mature MDC. Irradiation of immature MDC with 5 Gy of gamma-rays down regulated the expression of the costimulatory receptors CD80/CD86 and may compromise their ability to capture and present antigen. By contrast, gamma-Irradiation of mature MDC did not affect the expression of CD86/CD80, and HLA-DR. Gamma-irradiation increased the apoptosis of MDC; but did not affect the ability of mMDC to stimulate autologous MLR. T cell proliferative response in the MLR and in response to tetanus antigen was reduced when gamma-irradiated primary DC1 were used to either stimulate or present antigen to T cells.
Reuben
James M
JM
Department of Hematopathology, University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA. jreuben@mdanderson.org
Korbling
Martin
M
Gao
Hui
H
Lee
Bang-Ning
BN
D015703:Antigens, CD / Q000378:metabolism / Q000528:radiation effects; D017209:Apoptosis; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D002478:Cells, Cultured / Q000528:radiation effects; D003713:Dendritic Cells / Q000502:physiology* / Q000528:radiation effects*; D005720:Gamma Rays; D006684:HLA-DR Antigens / Q000378:metabolism; D007109:Immunity / Q000528:radiation effects; D008562:Membrane Glycoproteins; D009000:Monocytes / Q000528:radiation effects*; D019014:fas Receptor
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D006684:HLA-DR Antigens; D008562:Membrane Glycoproteins; D019014:fas Receptor