title: Production and characterization of active soluble human beta1,4-galactosyltransferase in Escherichia coli as a useful catalyst in synthesis of the Gal beta1-->4 GlcNAc linkage.
abstract: An active and soluble human beta1,4-galactosyltransferase (beta-GT) was produced in Escherichia coli using a maltose-binding protein fusion system. The purified recombinant beta-GT has a K(m) value of 0.035 mM for UDP-galactose and a V(max) of 643 x 10(3) nmol/mg/h. The enzyme catalyzes the transfer of galactose from UDP-galactose to N-linked oligosaccharides. The properties of the purified enzyme were identical to those of bovine milk beta-GT.
authors.affiliation: Medical Research Laboratory, Toyobo Co. Ltd., 2-1-1 Katata, Ohtsu, Shiga 520-0292, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shibatani
authors.forename: K
authors.initials: K
authors.lastname: Fujiyama
authors.forename: S
authors.initials: S
authors.lastname: Nishiguchi
authors.forename: T
authors.initials: T
authors.lastname: Seki
authors.forename: Y
authors.initials: Y
authors.lastname: Maekawa