Selective determination of gamma-aminobutyric acid, glutamate and alanine by mixed micellar electrokinetic chromatography and fluorescence detection.
A mixed micellar electrokinetic chromatography method with fluorescence detection was developed to simultaneously monitor gamma-aminobutyric acid (GABA), glutamate (Glu) and alanine (Ala) in biological samples. Amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA). The separation of three NDA-labeled isomers (GABA, alpha-ABA, beta-ABA) was studied in detail with different micelles solutions such as sodium dodecyl sulfate (SDS), beta-cyclodextrin (beta-CD) and sodium cholate (SC). Simultaneous resolution of GABA, Glu and Ala from 21 amino acids was achieved within 5 min using 20 mM phosphate buffer at pH 8.7 containing 24 mM SC and 26 mM SDS. The detection limits were 4.0 x 10(-8), 1.1 x 10(-8) and 1.3 x 10(-8) M, for GABA, Glu and Ala, respectively, with S/N = 2. The method was applied to monitor the changes of amount of GABA, Glu and Ala in tobacco leaf in response to cold and dark stress.
Zhang
Li-Yao
LY
Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, China.
Sun
Meng-Xiang
MX
D000409:Alanine / Q000032:analysis* / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary; D003080:Cold Temperature; D003624:Darkness; D018698:Glutamic Acid / Q000032:analysis* / Q000737:chemistry; D009281:Naphthalenes / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results; D014026:Nicotiana / Q000737:chemistry; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000737:chemistry
D009281:Naphthalenes; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; C027717:2,3-naphthalenedicarboxaldehyde; D000409:Alanine