Helicobacter pylori infection is associated with oxidatively damaged DNA in human leukocytes and decreased level of urinary 8-oxo-7,8-dihydroguanine.
Helicobacter pylori infection is responsible for inflammation, increased production of reactive oxygen species and oxidatively damaged DNA in the gastric mucosa. There is also evidence which suggests that H.pylori infection may lead to the development of several extragastroduodenal pathologies with reactive oxygen species involvement. In order to assess whether the infection may impose oxidatively damaged DNA not only in the target organ (stomach) but in other organs as well we decided, for the first time, to analyse the two kinds of oxidatively damaged DNA biomarkers: urinary excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanine (8-oxoGua) as well as the level of oxidatively damaged DNA in leukocytes. Using high performance liquid chromatography prepurification/gas chromatography with isotope dilution mass detection methodology, we examined the amount of oxidatively damaged DNA products excreted into urine and the amount of 8-oxodG in the DNA of leukocytes' (with the the HPLC/EC technique) in three groups of children: (i) control group, (ii) H.pylori infected children and (iii) children with gastritis where H.pylori infection was excluded. The levels of 8-oxodG in DNA isolated from leukocytes of H.pylori infected patients and in the group with gastritis without H.pylori infection were significantly higher than in DNA isolated from the control group. The mean level of 8-oxoGua in urine samples of children infected with H.pylori was significantly lower than in the urine of the group with gastritis without H.pylori infection. The data suggest that inflammation itself, not just H.pylori infection, is responsible for the observed rise of 8-oxodG level in leukocytes. However, the observed decrease in the level of modified base in urine seems to be specific for H.pylori infection and possibly linked with nitric oxide mediated inhibition of a key base excision repair enzyme (human 8-oxo-7, 8-dihydroguanine glycosylase) responsible for the repair of 8-oxo-7,8-dihydroguanine.
Siomek
Agnieszka
A
Department of Clinical Biochemistry, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Karlowicza 24, 85-092 Poland.
Rytarowska
Agnieszka
A
Szaflarska-Poplawska
Anna
A
Gackowski
Daniel
D
Rozalski
Rafal
R
Dziaman
Tomasz
T
Czerwionka-Szaflarska
Mieczyslawa
M
Olinski
Ryszard
R
D000293:Adolescent; D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D002648:Child; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D005260:Female; D005756:Gastritis / Q000503:physiopathology; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori / Q000472:pathogenicity; D006801:Humans; D007249:Inflammation; D007962:Leukocytes; D008297:Male; D009569:Nitric Oxide; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
D015415:Biomarkers; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; C024829:8-hydroxyguanine; D006147:Guanine