Cloning of full-length cDNA sequence of a novel candidate gene related to mouse cleft palate.
OBJECTIVE
Cloning and screening a novel candidate gene related to developing mouse cleft palate.


METHODS
The differentially expressed genes were cloned by modified PCR-based subtractive hybridization. After identification using reverse dot blotting, positive clones were sequenced and analyzed for homology in GenBank databases.


RESULTS
Four novel express sequence tags were obtained, one of which spanning 809 bp was the full-length of the novel gene cDNA identified by Northern hybridization.


CONCLUSIONS
A novel candidate gene related to mouse cleft palate was cloned.
Li
Xin
X
Department of Oral Pathology, Stomatology College, the Fourth Military Medical University, Xi'an, Shaanxi, 710032 PR China.
Jin
Yan
Y
Yan
Wei
W
Liu
Yuan
Y
Wang
Xin-wen
XW
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002972:Cleft Palate / Q000235:genetics* / Q000473:pathology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D017422:Sequence Analysis, DNA
D048868:Adaptor Proteins, Signal Transducing; D018076:DNA, Complementary; C515627:MCPR1 protein, mouse