Distribution of a novel protein AgK114 expression in the normal tissues of adult mice: dual expression of AgK114 and growth hormone in anterior pituitary cells.
The novel antigen K114 (AgK114) has been previously identified in normal hamster skin, and its expression has been up-regulated accompanying tissue damages of the skin, although there is no information on its biological functions. To determine the physiological role of AgK114, we prepared anti-mouse AgK114 monoclonal antibody and studied its tissue distribution in healthy adult mice by immunocytochemistry. A widespread and unique expression of AgK114 peptide was found in the selected organs of various systems (hair follicle cells and sebaceous gland of skin, ciliated epithelial cells of trachea and bronchial tube, striated portion of submandibular gland, distal convoluted tubule cells of kidney, ciliated epithelial cells of oviduct, medulla of adrenal gland and anterior lobe of pituitary gland). Interestingly, dual expression of AgK114 peptide and growth hormone in somatotrophs was found in anterior lobe of pituitary gland by double immunocytochemistry. AgK114 peptide was expressed widely in many regionally well-defined cellular systems in various peripheral tissues, suggesting that AgK114 peptide may have some roles of physiological functions in these organs. The data from our current study have provided a rationale for further studies of functional roles of AgK114 peptide in a variety of organs or tissues under physiological conditions.
Takeuchi
Makoto
M
Drugs, Cosmetics & Chemicals Development Center, Hayashibara Biochemical Laboratories Inc., Okayama, Japan. m_takeuchi@hayashibara.co.jp
Tatefuji
Tomoki
T
Kayano
Tohru
T
Okura
Takanori
T
Mori
Tetsuya
T
Ohta
Tsunetaka
T
Kurimoto
Masashi
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015153:Blotting, Western; D005260:Female; D015870:Gene Expression; D013006:Growth Hormone / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D010455:Peptides; D013006:Growth Hormone