title: Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli.
abstract: PriA, a DEXH-type DNA helicase, binds specifically to the 3' end of DNA through its N-terminal domain, and is a candidate sensor protein that recognizes arrested DNA replication forks in bacteria. We crystallized an N-terminal fragment of PriA in the absence and the presence of oligonucleotides to elucidate the structural basis for the specific recognition of the 3' terminus of DNA.
authors.affiliation: Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Fukuoka 812-8582, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Sasaki
authors.forename: Toyoyuki
authors.initials: T
authors.lastname: Ose
authors.forename: Taku
authors.initials: T
authors.lastname: Tanaka
authors.forename: Toshimi
authors.initials: T
authors.lastname: Mizukoshi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ishigaki
authors.forename: Katsumi
authors.initials: K
authors.lastname: Maenaka
authors.forename: Hisao
authors.initials: H
authors.lastname: Masai
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kohda
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D003460:Crystallization; D018360:Crystallography, X-Ray; D004265:DNA Helicases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments; D011994:Recombinant Proteins; D000251:Adenosine Triphosphatases; C506743:priA protein, E coli; D004265:DNA Helicases