[Regulatory volume decrease and its mechanism in nasopharyngeal epithelial cells].
To investigate regulatory volume decrease (RVD) and its mechanism in primary-culturing fetal human nasopharyngeal epithelial cells, living cell imaging technique was employed to detect the volume changes following exposure to hypotonic solution, and blockage of Cl- channels was used to clarify the role of Cl- channels in RVD. The results showed that extracellular hypotonic treatment swelled the cells and induced RVD. 47% hypotonic solution (160 mOsmol/L) swelled the cell by 144.7% and induced 38.7% recovery of cell volume within 20 min. RVD was correlated negatively to the extracellular osmolarity (r=-0.99, P<0.05) and positively to the swelling volume(r=0.99, P<0.05) in "S" shape, respectively. Chloride channel blockers, tamoxfen (20 micromol/ L), ATP (10 mmol/L) and NPPB (100 micromol/L), inhibited RVD by 100%, 76.3% and 62.7% (P< 0.01), respectively. The results indicated that primary-culturing fetal human nasopharyngeal epithelial cells are capable of RVD. Cl- efflux through Cl- channels is the key mechanism of RVD.
Sun
Xue Rong
XR
Laboratory of Physiology, Guangdong Medical College, Zhanjiang.
Chen
Li Xin
LX
Mao
Jian Wen
JW
Zhu
Lin Yan
LY
Nie
Si Huai
SH
Zhong
Ping
P
Li
Pan
P
Wang
Li Wei
LW
D000255:Adenosine Triphosphate / Q000494:pharmacology; D048429:Cell Size / Q000187:drug effects*; D002478:Cells, Cultured; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology; D009305:Nasopharynx / Q000166:cytology*; D009579:Nitrobenzoates / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology
D018118:Chloride Channels; D007038:Hypotonic Solutions; D009579:Nitrobenzoates; D013629:Tamoxifen; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D000255:Adenosine Triphosphate