title: The proangiogenic capacity of polymorphonuclear neutrophils delineated by microarray technique and by measurement of neovascularization in wounded skin of CD18-deficient mice.
abstract: Growing evidence supports the concept that polymorphonuclear neutrophils (PMN) are critically involved in inflammation-mediated angiogenesis which is important for wound healing and repair. We employed an oligonucleotide microarray technique to gain further insight into the molecular mechanisms underlying the proangiogenic potential of human PMN. In addition to 18 known angiogenesis-relevant genes, we detected the expression of 10 novel genes, namely midkine, erb-B2, ets-1, transforming growth factor receptor-beta2 and -beta3, thrombospondin, tissue inhibitor of metalloproteinase 2, ephrin A2, ephrin B2 and restin in human PMN freshly isolated from the circulation. Gene expression was confirmed by the RT-PCR technique. In vivo evidence for the role of PMN in neovascularization was provided by studying neovascularization in a skin model of wound healing using CD18-deficient mice which lack PMN infiltration to sites of lesion. In CD18-deficient animals, neovascularization was found to be significantly compromised when compared with wild-type control animals which showed profound neovascularization within the granulation tissue during the wound healing process. Thus, PMN infiltration seems to facilitate inflammation-mediated angiogenesis which may be a consequence of the broad spectrum of proangiogenic factors expressed by these cells.
authors.affiliation: Department of Physiology, Ludwig Maximilians University, Munich, Germany.
authors.forename: Ruth
authors.initials: R
authors.lastname: Schruefer
authors.forename: Silke
authors.initials: S
authors.lastname: Sulyok
authors.forename: Jurgen
authors.initials: J
authors.lastname: Schymeinsky
authors.forename: Thorsten
authors.initials: T
authors.lastname: Peters
authors.forename: Karin
authors.initials: K
authors.lastname: Scharffetter-Kochanek
authors.forename: Barbara
authors.initials: B
authors.lastname: Walzog
mesh_terms: D000328:Adult; D000818:Animals; D018821:CD18 Antigens / Q000235:genetics*; D051546:Cyclooxygenase 2 / Q000235:genetics; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D012867:Skin / Q000098:blood supply* / Q000293:injuries*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics; D014945:Wound Healing / Q000502:physiology
chemical_list: D018821:CD18 Antigens; D051546:Cyclooxygenase 2; D014568:Urokinase-Type Plasminogen Activator; D020780:Matrix Metalloproteinase 9