title: [Expression of hypoxia-inducible factor-1alpha in nasal polyps and its correlation with vascular endothelial growth factor and inducible nitric oxide synthase].
abstract: OBJECTIVE
  To detect the expression and distribution of hypoxia-inducible factor-1alpha (HIF-1alpha) in nasal polyps, to explore the relationship of HIF-1alpha with vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS), and to investigate the role of HIF-1alpha in the pathogenetic mechanism of nasal polyps.
  
  
  METHODS
  Using the techniques of immunohistochemistry and reverse transcription polymerase chain reaction, the expressions of HIF-1alpha, VEGF, iNOS protein and HIF-1alpha mRNA were examined. The specimens were obtained from patients underwent SMR surgery during the same period, including thirty-one nasal polyps and ten inferior turbinate tissues.
  
  
  RESULTS
  According to immunohistochemical research, the expressions of HIF-1alpha, VEGF and iNOS were higher in nasal polyps than those in inferior turbinate tissue (P < 0.01); The result of RT-PCR showed that there was no significant difference between the expression of HIF-1alpha mRNA in nasal polyps and inferior turbinate tissues (P > 0.05); No correlation was found between the expression of HIF-1alpha and the clinical stages of nasal polyps (F = 0.42, P > 0.05); There was a positive correlation between the expression of HIF-1alpha and that of VEGF (r = 0.630, P < 0.05). No significant correlation was found between the expression of HIF-1alpha and that of Inos (r = 0.144, P > 0.05).
  
  
  CONCLUSIONS
  Although the mRNA level of HIF-1alpha did not change much, the expression of its protein was significantly higher in nasal polyps than in inferior turbinate tissues. There was no correlation between the expression of HIF-1alpha and the clinical stages. A positive correlation was found between HIF-1alpha and VEGF, but no correlation was detected between the HIF-1alpha and iNOS. In conclusion, our study indicates that hypoxia may play an important role in the pathogenetic mechanism of nasal polyps.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. yangliu616@yahoo.com.cn
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Cui
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Yu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D055815:Young Adult
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II