Changes of the intestinal endocrine cells in the C57BL/6 mouse after implantation of murine lung carcinoma (3LL): an immunohistochemical quantitative study.
OBJECTIVE
To study the distributions and frequencies of intestinal endocrine cells in the C57BL/6 mouse with immunohistochemical method using seven types of specific antisera against chromogranin A (CGA), serotonin, somatostatin, glucagons, gastrin, cholecystokinin (CCK)-8 and human pancreatic polypeptide (hPP) after abdominal subcutaneous implantation of murine lung carcinoma (3LL).


METHODS
The experimental animals were divided into two groups, one is non-implanted Sham and the other is 3LL-implanted group. Samples were collected from six regions of intestinal tract at 28(th) d after implantation of 3LL cells (1X10(5) cell/mouse).


RESULTS
In this study, five types of immunoreactive (IR) cells were identified except for gastrin and hPP. The regional distributions of the intestinal endocrine cells in the 3LL-implanted group were similar to those of the non-implanted Sham. However, significant decreases of IR cells were detected in 3LL-implanted group compared to those of non-implanted Sham. CGA- and serotonin-IR cells significantly decreased in 3LL-implanted groups compared to that of non-implanted Sham. Somatostatin-IR cells in the jejunum and ileum and CCK-8-IR cells in the jejunum of 3LL-implanted groups significantly decreased compared to that of non-implanted Sham. In addition, glucagon-IR cells were restricted to the ileum and colon of non-implanted Sham.


CONCLUSIONS
Implantation of tumor cell mass (3LL) induced severe quantifiable changes of intestinal endocrine cell density and the abnormality in density of intestinal endocrine cells may contribute to the development of gastrointestinal symptoms such as anorexia and indigestion, frequently encountered in patients with cancer.
Ku
Sae-Kwang
SK
Pharmacology and Toxicology Laboratory, Central Research Laboratories, Dong-Wha Pharmaceutical Industrial Co, Ltd, Anyang 430-017, Republic of Korea.
Seong
Seung-Kyoo
SK
Kim
Dae-Young
DY
Lee
Hyeung-Sik
HS
Kim
Jong-Dae
JD
Choi
Hae-Yun
HY
Seo
Bu-Il
BI
Lee
Jae-Hyun
JH
D000818:Animals; D045744:Cell Line, Tumor; D019858:Enteroendocrine Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005768:Gastrointestinal Hormones / Q000378:metabolism; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D036361:Peptide Hormones / Q000378:metabolism
D005768:Gastrointestinal Hormones; D036361:Peptide Hormones