title: Combined NMR-observation of cold denaturation in supercooled water and heat denaturation enables accurate measurement of deltaC(p) of protein unfolding.
abstract: Cold and heat denaturation of the double mutant Arg 3-->Glu/Leu 66-->Glu of cold shock protein Csp of Bacillus caldolyticus was monitored using 1D (1)H NMR spectroscopy in the temperature range from -12 degrees C in supercooled water up to +70 degrees C. The fraction of unfolded protein, f (u), was determined as a function of the temperature. The data characterizing the unfolding transitions could be consistently interpreted in the framework of two-state models: cold and heat denaturation temperatures were determined to be -11 degrees C and 39 degrees C, respectively. A joint fit to both cold and heat transition data enabled the accurate spectroscopic determination of the heat capacity difference between native and denatured state, DeltaC(p) of unfolding. The approach described in this letter, or a variant thereof, is generally applicable and promises to be of value for routine studies of protein folding.
authors.affiliation: Department of Chemistry, The State University of New York, Buffalo, NY 14260, USA. szypersk@chem.buffalo.edu
authors.forename: Thomas
authors.initials: T
authors.lastname: Szyperski
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Mills
authors.forename: Dieter
authors.initials: D
authors.lastname: Perl
authors.forename: Jochen
authors.initials: J
authors.lastname: Balbach
mesh_terms: D000465:Algorithms; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003080:Cold Temperature; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics; D006358:Hot Temperature; D009682:Magnetic Resonance Spectroscopy; D009154:Mutation; D011489:Protein Denaturation; D017510:Protein Folding; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; C407094:cold shock protein, Bacillus; D014867:Water