Production of an immunosuppressive polysaccharide, AZ9, in the culture of Klebsiella oxytoca strain TNM3.
A soil bacterium, Klebsiella oxytoca TNM3, was found to produce a polysaccharide named AZ9 with immunosuppressive activities. The culture of K. oxytoca in a jar fermentor resulted in an extreme increase in the viscosity of the culture broth (>10,000 mPa.s) due to accumulation of the polysaccharide with a high molecular weight. The molecular weight of AZ9 was successfully controlled by supplementing ethylenediamine tetraacetic acid (EDTA) and Fe2+ ion to the culture. Under optimized culture conditions, 39 g/l AZ9 with an average molecular weight of 200 kDa was produced, which was effective in suppressing IgE antibody production in mice.
Sugihara
R
R
Tayca Corporation, Taisho-ku, Osaka 551, Japan.
Oiso
Y
Y
Matsumoto
Y
Y
Ohmori
H
H