Effects of mupirocin at subinhibitory concentrations on biofilm formation in Pseudomonas aeruginosa.
BACKGROUND
Subinhibitory concentrations of mupirocin can suppress flagellar formation in Pseudomonas aeruginosa. In this study, we evaluated the effect of mupirocin on biofilm formation in P. aeruginosa.


METHODS
P. aeruginosa PAO-1 (MIC, >1,024 microg/ml for mupirocin) was used. Viable bacteria adhering or forming biofilm on a Cell Desk were counted and observed by scanning electron microscopy.


RESULTS
With or without continuous exposure to 256 microg/ml of mupirocin, counts of adherent and/or biofilm-forming bacteria showed no difference until day 10. Whereas biofilm formation with glycocalyx was observed on day 6-10 without mupirocin, mupirocin reduced biofilm formation and glycocalyx production for 10 days.


CONCLUSIONS
Subinhibitory concentrations of mupirocin can reduce biofilm formation in vitro in P. aeruginosa.
Ishikawa
Jinko
J
Department of Laboratory Medicine, Hamamatsu University School of Medicine, Japan.
Horii
Toshinobu
T
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D016712:Mupirocin / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D016712:Mupirocin