title: [Expression of SARS-CoV in various types of cells in lung tissues].
abstract: OBJECTIVE
  To investigate the cell types infected by severe acute respiratory syndrome-associated coronavirus (SARS-CoV) in lung tissues and explore the mechanism of lung injury in SARS.
  
  
  METHODS
  In-situ hybridization(ISH) and immunohistochemistry(IHC) double staining was applied to study the lung tissues from 7 SARS cases of Beijing and one of Anhui province. According to SARS-CoV genome sequence, the cDNA probe was synthesized and labelled by digoxin. Immunohistochemically, antibodies of cytokeratin(CK), CD34, CD68, Vimentin and CD3 were applied to demonstrate bronchial epithelial cells, type II pneumocytes, endothelial cells, macrophages, fibroblasts and T cells respectively.
  
  
  RESULTS
  The positive results of in-situ hybridization showed that the lung tissues of all cases expressed SARS-CoV RNA, and positive signals displayed in cytoplasms (purple-blue, NBP-BCIP. ISH-IHC double staining showed that positive signals of both ISH (purple-blue NBT-BCIP and IHC (red-brown, AEC expressed in the cytoplasms (purple and red). The positive results of double staining indicated that bronchial epithelial cells, type II pneumocytes, endothelial cells, macrophages, fibroblasts and T lymphocytes were diffusely infected by SARS-CoV.
  
  
  CONCLUSIONS
  This study of ISH-IHC double staining in lung tissues of SARS patients showed that bronchial epithelial cells, type II pneumocytes, endothelial cells, macrophages, T lymphocytes and fibroblasts were attacked diffusely in SARS lungs. Various types of cells damaged by SARS-CoV and inflammatory mediators released by those cells play an important role in the pathogenesis of lung injury in SARS.
authors.affiliation: Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100083, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
authors.forename: En-cong
authors.initials: EC
authors.lastname: Gong
authors.forename: Qian-ying
authors.initials: QY
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: Gu
authors.forename: Xing-wang
authors.initials: XW
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.forename: Hong-quan
authors.initials: HQ
authors.lastname: Shao
authors.forename: Zi-fen
authors.initials: ZF
authors.lastname: Gao
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Wei-gang
authors.initials: WG
authors.lastname: Fang
authors.forename: Yan-feng
authors.initials: YF
authors.lastname: Zhong
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007633:Keratins / Q000032:analysis; D008168:Lung / Q000737:chemistry / Q000473:pathology / Q000821:virology*; D016676:Macrophages, Alveolar / Q000737:chemistry / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000302:isolation & purification*; D045169:Severe Acute Respiratory Syndrome / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D013601:T-Lymphocytes / Q000737:chemistry / Q000473:pathology / Q000821:virology; D014746:Vimentin / Q000032:analysis; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; D017252:CD3 Complex; D007633:Keratins; D012367:RNA, Viral; D014746:Vimentin; C067980:CD68 antigen, human; D000097382:CD68 Molecule