title: Isolation and Characterization of an Outer Membrane Protein of Chlorobium tepidum.
abstract: A protein was isolated from membranes of the green sulfur bacterium Chlorobium tepidum. This protein was characterized by gel electrophoresis, gel filtration, analytical ultracentrifugation and amino acid sequencing. The molecular weight of the purified protein was shown to be 26 kDa by SDS-PAGE. HPLC gelfiltration, SDS-PAGE and analytical ultracentrifugation are consistent with the presence of a homogenous protein in the preparations. Amino acid analysis was obtained from the isolated protein after fragmentation with Lys-C, trypsin and cyanogen bromide. The cleavage pattern resulting from these treatments combined with Edman sequencing yield a sequence allowing the identification of an integral membrane agglutinin in Chl. tepidum.
authors.affiliation: Department of Chemistry, Division of Biochemistry, University of Crete, Leoforos Knossou, 71409, Heraklion, Greece.
authors.forename: Michalis
authors.initials: M
authors.lastname: Aivaliotis
authors.affiliation: Department of Chemistry, Division of Biochemistry, University of Crete, Leoforos Knossou, 71409, Heraklion, Greece.
authors.forename: Elefteria
authors.initials: E
authors.lastname: Neofotistou
authors.affiliation: Department of Chemistry, Division of Biochemistry, University of Crete, Leoforos Knossou, 71409, Heraklion, Greece.|Biozentrum, University of Basel, Klingelbergstr. 70, 4056, Basel, Switzerland.
authors.forename: Hervé-W
authors.initials: HW
authors.lastname: Rémigy
authors.affiliation: Department of Chemistry, Division of Biochemistry, University of Crete, Leoforos Knossou, 71409, Heraklion, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Tsimpinos
authors.affiliation: Biozentrum, University of Basel, Klingelbergstr. 70, 4056, Basel, Switzerland.
authors.forename: Ariel
authors.initials: A
authors.lastname: Lustig
authors.affiliation: Max Planck Institute for Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Lottspeich
authors.affiliation: Department of Chemistry, Division of Biochemistry, University of Crete, Leoforos Knossou, 71409, Heraklion, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Tsiotis