Isolation from Gluconacetobacter diazotrophicus cell walls of specific receptors for sugarcane glycoproteins, which act as recognition factors.
Glycoproteins from sugarcane stalks have been isolated from plants field-grown by size-exclusion chromatography. Some of these glycoproteins, previously labelled with fluorescein isothiocyanate, are able to bind to the cell wall of the sugarcane endophyte, N2-fixing Gluconacetobacter diazotrophicus, and largely removed after washing the bacterial cells with sucrose. This implies that sugarcane glycoproteins use beta-(1-->2)-fructofuranosyl fructose domains in their glycosidic moiety to bind to specific receptors in the bacterial cell walls. These receptors have been isolated by affinity chromatography on a sugarcane glycoprotein-agarose matrix, desorbed with sucrose and characterized by sodium dodecyl sulfate polyacrylamide gel electrophresisand capillary electrophoresis (CE).
Blanco
Y
Y
Laboratory of Plant Physiology, Faculty of Biology, Complutense University, 28040 Madrid, Spain.
Arroyo
M
M
Legaz
M E
ME
Vicente
C
C
D002473:Cell Wall / Q000737:chemistry*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D041821:Gluconacetobacter / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000302:isolation & purification* / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry*
D005456:Fluorescent Dyes; D006023:Glycoproteins; D011956:Receptors, Cell Surface