Transfer of high-mannose-type oligosaccharides to disaccharides by endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae.
Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) has transglycosylation activity, and high-mannose-type oligosaccharides are transferred to suitable glycosides as acceptor substrates. The acceptor specificity of Endo-A-catalyzed transglycosylation toward various disaccharides was investigated. To identify an effective acceptor for the transglycosylation by Endo-A, the reaction was carried out using various disaccharides. Endo-A transferred high-mannose-type oligosaccharides more efficiently to beta-linked disaccharides (cellobiose, gentiobiose, sophorose, and laminaribiose) than to alpha-linked disaccharides (isomaltose, maltose, nigerose, kojibiose, and trehalose) as acceptor substrates. The transglycosylation products, (Man)6GlcNAc-Glc-beta-Glc, were more rapidly hydrolyzed than (Man)6GlcNAc-Glc-alpha-Glc. These results indicate that Endo-A recognizes the anomeric configuration of the acceptor substrates, and beta-linked glycosides are suitable for the synthesis of transglycosylation products.
Fujita
Kiyotaka
K
Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.
Miyamura
Tsuyoshi
T
Sano
Mutsumi
M
Kato
Ikunoshin
I
Takegawa
Kaoru
K