Enhanced production of prodigiosin-like pigment from Serratia marcescens SMdeltaR by medium improvement and oil-supplementation strategies.
Serratia marcescens SMdeltaR, an SpnR-defective isogenic mutant of S. marcescens SS-1, was used to produce a prodigiosin-like pigment (PLP). Luria-Bertani (LB) broth, frequently used for prodigiosin biosynthesis with S. marcescens strains, was modified by increasing the concentrations of tryptone and yeast extract while completely removing NaCl from the medium. The resulting modified LB (MLB) medium achieved an almost 3.0-fold increase in PLP yield (152 mg l(-1)) when compared with the original LB broth. The addition of vegetable oils (2-6% [v/v]) to the fermentation broth markedly enhanced PLP production. PLP yields of 525, 579, and 790 mg l(-1) were obtained when the MLB medium was supplemented with 4% soybean oil, 4% olive oil and 6% sunflower oil, respectively. PLP production was found to be positively correlated with extracellular surface emulsification activity, suggesting a link between the PLP production and the presence of biosurfactant. This work shows that the optimal medium for PLP yield was sunflower oil (6%)-supplemented MLB medium, which resulted in an approximately 14-fold higher PLP yield than that in LB broth. Mass spectrometry and NMR analysis indicated that the PLP product is a prodigiosin derivative, called undecylprodigiosin.
Wei
Yu-Hong
YH
Graduate School of Biotechnology and Bioinformatics, Yuan-Ze University, Chung-Li, Taoyuan 320, Taiwan. yhwei@saturn.yzu.edu.tw
Chen
Wei-Chuan
WC
D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D010938:Plant Oils / Q000378:metabolism*; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D012706:Serratia marcescens / Q000254:growth & development / Q000378:metabolism*
D010938:Plant Oils; C047347:undecylprodigiosin; D011353:Prodigiosin