title: Mutant presenilin 2 increases acetylcholinesterase activity in neuronal cells.
abstract: A presenilin 2 mutation is believed to be involved in the development of Alzheimer's disease. In addition, transgenic mice with a presenilin 2 mutation have been reported to have learning and memory impairments. In this study, exposing PC12 cells expressing mutant presenilin 2 to 50 microM AP25-35, 30 mM L-glutamate and 50 microM H2O2 caused a significant increase in acetylcholine esterase activity. An in vivo study revealed high levels of this enzyme activity in the mutant presenilin 2 transgenic brains compared with the wild type presenilin 2 transgenic and nontransgenic samples. These results suggest that a mutant presenilin 2-induced neurodegeneration in Alzheimer's disease might be involved in the increase in acetylcholinesterase activity. These findings might help in the development of an appropriate therapeutic intervention targeting mutant presenilin 2-induced Alzheimer's disease.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Hong Nga
authors.initials: HN
authors.lastname: Nguyen
authors.forename: Dae Youn
authors.initials: DY
authors.lastname: Hwang
authors.forename: Young Kyu
authors.initials: YK
authors.lastname: Kim
authors.forename: Do Young
authors.initials: DY
authors.lastname: Yoon
authors.forename: Jae Hwa
authors.initials: JH
authors.lastname: Kim
authors.forename: Moon Soon
authors.initials: MS
authors.lastname: Lee
authors.forename: Myung Koo
authors.initials: MK
authors.lastname: Lee
authors.forename: Yeo Pyo
authors.initials: YP
authors.lastname: Yun
authors.forename: Ki Wan
authors.initials: KW
authors.lastname: Oh
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000544:Alzheimer Disease / Q000188:drug therapy / Q000201:enzymology / Q000209:etiology; D000818:Animals; D001921:Brain / Q000201:enzymology; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D009474:Neurons / Q000201:enzymology*; D016716:PC12 Cells; D053766:Presenilin-2; D051381:Rats
chemical_list: D008565:Membrane Proteins; D053766:Presenilin-2; D000110:Acetylcholinesterase