Occurrence and distribution of endobacteria in the plant-associated mycelium of the ectomycorrhizal fungus Laccaria bicolor S238N.
Fluorescence in situ hybridization, associated with confocal laser scanning microscopy or epifluorescence microscopy with deconvolution system, has allowed the detection of a community of intracellular bacteria in non-axenic samples of the ectomycorrhizal fungus Laccaria bicolor S238N. The endobacteria, mainly alpha-proteobacteria, were present in more than half of the samples, which consisted of ectomycorrhizae, fungal mats and fruit bodies, collected in the glasshouse or in the forest. Acridine orange staining suggests that the endobacteria inhabit both live and dead fungal cells. The role of these endobacteria remains to be clarified.
Bertaux
J
J
UMR INRA-UHP Interactions Arbres/Micro-organismes, Centre INRA de Nancy, 54280 Champenoux, France.
Schmid
M
M
Hutzler
P
P
Hartmann
A
A
Garbaye
J
J
Frey-Klett
P
P
D000165:Acridine Orange; D000363:Agaricales; D020561:Alphaproteobacteria / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D038821:Mycorrhizae; D009841:Oligonucleotides; D018517:Plant Roots / Q000382:microbiology*; D028224:Pseudotsuga / Q000382:microbiology; D029963:Quercus / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D007211:Indoles; D009841:Oligonucleotides; D012336:RNA, Ribosomal, 16S; C007293:DAPI; D000165:Acridine Orange