title: [Spontaneous and induced programmed cell death in suspension cell cultures of cotton (Gossypium hirsutum L.)].
abstract: Cotton suspension cells grew well in the MS medium supplemented with 0.1 mg/L 2,4 D and 0.1 mg/L KT. Senescence occurred when the cells were unsubcultured. The cells began to lose their viabilities on the 17th day, and on the 21th day oligonucleosomal sized DNA fragments ( DNA ladder) could be detected. Oligonucleosomal sized DNA fragments ( DNA ladder) was the hallmark of the programmed cell death. Programmed cell death of cotton suspension cells could be induced respectively by some stress factors which included heatshock (42+/-3 degrees C for 8 hours), 10 micromol/L camptothecin, 20 micromol/L fumonisin B1 and 50 mmol/L cycloheximide. The cotton suspension cells which grew in the MS medium supplemented with 0.1 mg/L 2,4 D and 0.1 mg/L KT differred physiologically from the cells in the MS medium supplemented with 0.1 mg/L IBA and 0.1 mg/L KT, and they responded differentially to the heatshock, 10 micromol/L camptothecin and 20 micromol/L fumonisin B1, while the same to 50 mmol/L cycloheximide.
authors.affiliation: National Key Laborotory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Qi Zhong
authors.initials: QZ
authors.lastname: Xia
authors.forename: Xian Long
authors.initials: XL
authors.lastname: Zhang
authors.forename: Yi Chun
authors.initials: YC
authors.lastname: Nie
authors.forename: Xiao Ping
authors.initials: XP
authors.lastname: Guo
authors.forename: Long Fu
authors.initials: LF
authors.lastname: Zhu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects; D018744:DNA, Plant / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D037341:Fumonisins / Q000494:pharmacology; D003368:Gossypium / Q000166:cytology* / Q000187:drug effects / Q000235:genetics; D006358:Hot Temperature
chemical_list: D018744:DNA, Plant; D037341:Fumonisins; C056933:fumonisin B1; D003513:Cycloheximide; D002166:Camptothecin