A fluorimetric and circular dichroism study of hemoglobin--effect of pH and anionic amphiphiles.
In this work, bovine hemoglobin (Hb) has been studied mainly by the fluorescence method. pH has been found to exert a profound effect on Hb structure. This has been confirmed by fluorescence and circular dichroism (CD) studies. The pH-induced change in quaternary structure of Hb indirectly affects its secondary structure. This in turn affects ligand binding to Hb at various pH. The binding of two amphiphiles, a bile salt and a surfactant, have been investigated. The pH-induced structural modification of Hb has been confirmed by studies with the well-known denaturant urea and the polarity probe ANS, which has been used as an extrinsic fluorophore.
De
Swati
S
Department of Chemistry, University of Kalyani, Kalyani 741235, India. swati_de1@rediffmail.com
Girigoswami
Agnishwar
A
D000818:Animals; D000838:Anions; D002417:Cattle; D002942:Circular Dichroism; D006454:Hemoglobins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D020358:Sodium Cholate / Q000737:chemistry* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism; D014508:Urea / Q000737:chemistry* / Q000378:metabolism
D000838:Anions; D006454:Hemoglobins; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D014508:Urea; D020358:Sodium Cholate