[The assay of tissue collagenase activity using fluorescein isothiocyanate labeled collagen].
This report describes the assay of collagenolytic activity, particularly activity of tissue collagenases, using the reconstituted fibrils of fluorescein-labeled collagen type I as substrate. Labeling of soluble rat skin collagen was carried out by the modified method of Baichi et al 1980. This method is very sensitive, reproducible and useful for screening large number of samples.
Ryzhakova
O S
OS
Solov'eva
N I
NI
D000818:Animals; D003094:Collagen / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000032:analysis* / Q000378:metabolism; D003012:Microbial Collagenase / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012867:Skin / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D003094:Collagen; D003012:Microbial Collagenase; D020781:Matrix Metalloproteinase 1; D016650:Fluorescein-5-isothiocyanate