Characterization of surface sugars on algal cells with fluorescein isothiocyanate-conjugated lectins.
We used qualitative and quantitative fluorescence microscopy of the fluorescein isothiocyanate-conjugated lectins Concanavalin A, phytohaemagglutinin-erythroagglutinin, pokeweed mitogen, and peanut agglutinin to examine sugar composition on the cell surface and cell-associated mucilage (where present) in a number of cultured and environmental algae. Lectin-binding activity was markedly different between laboratory-cultured and environmental samples of the same species. Sugar composition of the cyanobacterium Anabaena cylindrica varied with growth cycle, although no clear pattern of change was observed. Akinetes typically showed lectin-binding activity higher than that of the vegetative cells or heterocysts throughout the growth cycle. Algae with mucilage showed greater lectin binding, indicating that mucilage contained more surface sugars accessible to the lectin probe compared with the cell wall surface. A low level of galactose and N-acetyl galactosamine (detected by peanut agglutinin) was associated with the surface mucilage of most algal species. Relatively high amounts of mannose, glucose, and N-acetyl glucosamine (detected by Concanavalin A, phytohaemagglutinin, and pokeweed mitogen) were also present. Lectin binding was shown to be a highly specific and sensitive approach to the examination of cell surface chemistry of both cultured and environmental algae and to the study of biodiversity in phytoplankton.
Tien
C-J
CJ
Department of Industrial Safety and Hygiene, Chung Hwa College of Medical Technology, Jen-Te Hsiang.
Sigee
D C
DC
White
K N
KN
D046868:Anabaena cylindrica / Q000254:growth & development / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence
D002241:Carbohydrates; D037102:Lectins; D016650:Fluorescein-5-isothiocyanate