A preprocessing method for inferring genetic interaction from gene expression data using Boolean algorithm.
Unknown genetic regulation mechanisms are expected to be discovered by information technology using large amount of biological data especially for gene expression data. In this study, we propose a novel inferring method for genetic interactions that combines our original preprocessing method and the Boolean algorithm. First, the performance of our method was evaluated using artificial data. The results showed that our method was able to infer genetic interactions with high specificity (specificity=0.629). Then, using our method, the genetic interaction was inferred from the experimental time course data collected using microarray on 69 genes of cell cycle for Saccharomyces cerevisiae. Our method estimated about 80% of all genetic interactions in Kyoto Encyclopedia Genes and Genomes (KEGG) for these genes. Furthermore, our method was able to infer several other genetic interactions that are not included in KEGG but whose existence is supported by other biological reports.
Hakamada
Kazumi
K
Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Hanai
Taizo
T
Honda
Hiroyuki
H
Kobayashi
Takeshi
T