DNA topoisomerase II interacts with Lim15/Dmc1 in meiosis.
Lim15/Dmc1 is a meiosis specific RecA-like protein. Here we propose its participation in meiotic chromosome pairing-related events along with DNA topoisomerase II. Analysis of protein-protein interactions using in vitro binding assays provided evidence that Coprinus cinereus DNA topoisomerase II (CcTopII) specifically interacts with C.cinereus Lim15/Dmc1 (CcLim15). Co-immunoprecipitation experiments also indicated that the CcLim15 protein interacts with CcTopII in vivo. Furthermore, a significant proportion of CcLim15 and CcTopII could be shown to co-localize on chromosomes from the leptotene to the zygotene stage. Interestingly, CcLim15 can potently activate the relaxation/catenation activity of CcTopII in vitro, and CcTopII suppresses CcLim15-dependent strand transfer activity. On the other hand, while enhancement of CcLim15's DNA-dependent ATPase activity by CcTopII was found in vitro, the same enzyme activity of CcTopII was inhibited by adding CcLim15. The interaction of CcLim15 and CcTopII may facilitate pairing of homologous chromosomes.
Iwabata
Kazuki
K
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Koshiyama
Akiyo
A
Yamaguchi
Taiki
T
Sugawara
Hiroko
H
Hamada
Fumika N
FN
Namekawa
Satoshi H
SH
Ishii
Satomi
S
Ishizaki
Takashi
T
Chiku
Hiroyuki
H
Nara
Takayuki
T
Sakaguchi
Kengo
K
D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry / Q000201:enzymology; D003302:Coprinus / Q000201:enzymology; D004250:DNA Topoisomerases, Type II / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D047468:Immunoprecipitation; D008540:Meiosis; D008969:Molecular Sequence Data; D017384:Sequence Deletion; D020798:Two-Hybrid System Techniques
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D004250:DNA Topoisomerases, Type II