Determination of fexofenadine in tablets by capillary electrophoresis in free solution and in solution with cyclodextrins as analyte carriers.
Capillary electrophoresis (CE) methods for the determination of fexofenadine (FEX) in commercial pharmaceuticals were developed. It was demonstrated that FEX could be effectively analyzed in free solution cationic CE at low pH. Another analytical approach studied was based on cyclodextrin (CD) modified CE where highly charged CD derivatives served as analyte carriers. In this way, the separation range was spread to physiological pH region and a CE analysis of FEX, present actually in its zwitterionic form, could be accomplished. Several parameters affecting the separations were studied, including the type and concentration of carrier ion, counterion, analyte carrier, and pH of the buffer. The methods based on the free solution CE and CD-modified CE were compared each other, validated, and applied for the determination of FEX in tablets.
Mikus
Peter
P
Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic. mikus@fpharm.uniba.sk
Valásková
Iva
I
Havránek
Emil
E
D018926:Anti-Allergic Agents / Q000032:analysis; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004337:Drug Carriers; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary; D006634:Histamine H1 Antagonists / Q000032:analysis; D013607:Tablets; D016593:Terfenadine / Q000031:analogs & derivatives* / Q000032:analysis; D047392:beta-Cyclodextrins / Q000737:chemistry*
D018926:Anti-Allergic Agents; D004337:Drug Carriers; D004573:Electrolytes; D006634:Histamine H1 Antagonists; C062590:O-carboxymethyl-O-ethyl-beta-cyclodextrin; D013607:Tablets; D047392:beta-Cyclodextrins; D016593:Terfenadine; C093230:fexofenadine; C031215:betadex