Size sorting of protein assemblies using polymeric gradient surfaces.
We report on a novel approach for the size-dependent fractionation of protein assemblies on polymeric surfaces. Using a simple temperature gradient method to generate one-dimensional gradients of grafted poly(ethylene glycol), we fabricated silicon-oxide chips with a gradually changing surface density of kinesin motor molecules. We demonstrate that such a bioactive surface can be used to sort gliding microtubules according to their length. To our knowledge, this is the first example of the self-organized sorting of protein assemblies on surfaces.
Ionov
Leonid
L
Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Stamm
Manfred
M
Diez
Stefan
S
D005591:Chemical Fractionation / Q000379:methods*; D016547:Kinesins / Q000737:chemistry / Q000302:isolation & purification*; D046208:Microchip Analytical Procedures; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008870:Microtubules / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D010087:Oxides / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013696:Temperature
D008869:Microtubule-Associated Proteins; D010087:Oxides; D016547:Kinesins; D012825:Silicon