A multivalent HIV-vaccine: development of a plasmid DNA for the expression of HIV envelope glycoproteins with hypervariable V3-loop domains.
The third hypervariable (V3) loop of the HIV-1 envelope glycoprotein gp120 plays an essential role in the process of viral entry. It contributes to the tropism, coreceptor usage and immune-escape of the virus. We generated a monovalent plasmid DNA and demonstrated the expression of HIV-1 clade B subtype NL4-3 gp120 and gp160 in comparison to a multivalent plasmid DNA encoding for a variety of V3-variants. In contrast to the membrane-anchored gp160, preliminary data demonstrate the monovariant gp120 is expressed in and presented on a human dendritic cell (DC) line, due to a HIVenv-specific re-stimulation of naïve T-cells detected by IFNgamma-ELISPOT assay.
Schilling
R
R
Strathmann Biotec AG, Habichthorst 30, Hamburg 22459, Germany. ralf.schilling@strathmann.de
Heil
A
A
Langner
K
K
Pohlmeyer
K
K
Larsen
M
M
Baumeister
H
H
Goletz
S
S
Behnke
B
B
D016915:AIDS Vaccines; D000595:Amino Acid Sequence; D015699:HIV Envelope Protein gp120 / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
D016915:AIDS Vaccines; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins