In vitro and in vivo two-photon luminescence imaging of single gold nanorods.
Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos(4) dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.
Wang
Haifeng
H
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
Huff
Terry B
TB
Zweifel
Daniel A
DA
He
Wei
W
Low
Philip S
PS
Wei
Alexander
A
Cheng
Ji-Xin
JX
D000818:Animals; D001775:Blood Circulation; D001808:Blood Vessels; D003952:Diagnostic Imaging / Q000379:methods*; D004423:Ear / Q000098:blood supply; D005260:Female; D005454:Fluorescence Polarization; D006046:Gold; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal / Q000379:methods; D049329:Nanostructures
D006046:Gold