title: Induction of liver cytochrome P450 1A2 expression by flutamide in rats.
abstract: OBJECTIVE
  To investigate the modulation of liver cytochrome P450 1A2 (CYP1A2) expression by giving flutamide to adult rats.
  
  
  METHODS
  Rats were given 50, 100, and 200 mg/kg po of flutamide for 2 weeks. Liver CYP1A2 mRNA was measured using reverse transcription-polymerase chain reaction. CYP1A2 protein was detected using immunoblotting. CYP1A2 activity was assayed using high performance liquid chromatography, with caffeine as the CYP1A2 substrate.
  
  
  RESULTS
  CYP1A2 mRNA levels after flutamide treatment at 100 mg/kg and 200 mg/kg were, respectively, 1.86 and 3.11-fold higher than those of the control. Correspondingly, CYP1A2 protein increased 1.78 and 2.89-fold and CYP1A2 activity increased approximately 1.65 and 2.83-fold, respectively, relative to controls. Flutamide treatment at 50 mg/kg had no significant effect on CYP1A2 mRNA, protein, or enzyme activity.
  
  
  CONCLUSIONS
  Giving rats flutamide induced liver CYP1A2 expression in a dose-dependent manner.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 200032, China.
authors.forename: Hai-xue
authors.initials: HX
authors.lastname: Wang
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Chang-jiang
authors.initials: CJ
authors.lastname: Xu
authors.forename: Xiao-chao
authors.initials: XC
authors.lastname: Ma
authors.forename: Jian-er
authors.initials: JE
authors.lastname: Long
authors.forename: Duan
authors.initials: D
authors.lastname: Li
mesh_terms: D000726:Androgen Antagonists / Q000633:toxicity; D000818:Animals; D019388:Cytochrome P-450 CYP1A2 / Q000096:biosynthesis* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005485:Flutamide / Q000008:administration & dosage / Q000633:toxicity*; D008099:Liver / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000726:Androgen Antagonists; D012333:RNA, Messenger; D005485:Flutamide; D019388:Cytochrome P-450 CYP1A2