title: Development and evaluation of a highly sensitive human papillomavirus genotyping DNA chip.
abstract: OBJECTIVE
  Test of human papillomavirus (HPV) is a useful adjunctive tool of Pap smear to screen cervical cancer. We have developed a novel HPV genotyping DNA chip arrayed by multiple oligonucleotide probes of both L1 and E6/E7 gene sequence of 42 types of anogenital HPV.
  
  
  METHODS
  Consensus PCR products of L1 and E6/E7 gene sequences of HPV are hybridized to arrayed probes on the HPV chip and HPV genotypes are identified by fluorescence scanner. We have comparatively analyzed the value of HPV DNA chip and DNA sequencing in 100 cervical cancer tissues.
  
  
  RESULTS
  Overall, 98 cervical cancer tissues were found to harbor DNA sequences of high-risk type HPVs, of which 88 (89.8%) were detected by PCR-sequencing of L1 alone, 98 (100%) by PCR-sequencing of both L1 and E6/E7, and 98 (100%) by HPV DNA chip, respectively. All of the genotypes of HPV detected on sequencing analysis were also found on DNA chip analysis. HPV DNA chip was superior to direct DNA sequencing in detection of mixed infection.
  
  
  CONCLUSIONS
  These results suggest that HPV DNA chip analysis in the present study is highly accurate for detection and genotyping of HPV and may have potential value as a robust, high-throughput screening test of uterine cervix cancer.
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Ki Hyung
authors.initials: KH
authors.lastname: Kim
authors.forename: Man Soo
authors.initials: MS
authors.lastname: Yoon
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Na
authors.forename: Chang Soo
authors.initials: CS
authors.lastname: Park
authors.forename: Myung Ryurl
authors.initials: MR
authors.lastname: Oh
authors.forename: Woo Chul
authors.initials: WC
authors.lastname: Moon
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D017124:Conserved Sequence; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications* / Q000821:virology*; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000821:virology*
chemical_list: D004273:DNA, Neoplasm; D004279:DNA, Viral