Hepatoprotective effects of rubiadin, a major constituent of Rubia cordifolia Linn.
The hepatoprotective effects of rubiadin, a major constituent isolated from Rubia cordifolia Linn., were evaluated against carbon tetrachloride (CCl4)-induced hepatic damage in rats. Rubiadin at a dose of 50, 100 and 200 mg/kg was administered orally once daily for 14 days. The substantially elevated serum enzymatic activities of serum glutamic oxaloacetic transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), serum alkaline phosphatase (SALP) and gamma-glutamyltransferase (gamma-GT) due to carbon tetrachloride treatment were dose dependently restored towards normalization. Meanwhile, the decreased activities of glutathione S-transferase and glutathione reductase were also restored towards normalization. In addition, rubiadin also significantly prevented the elevation of hepatic malondialdehyde formation and depletion of reduced glutathione content in the liver of CCl4 intoxicated rats in a dose dependent manner. Silymarin used as standard reference also exhibited significant hepatoprotective activity on post treatment against carbon tetrachloride induced hepatotoxicity in rats. The biochemical observations were supplemented with histopathological examination of rat liver sections. The results of this study strongly indicate that rubiadin has a potent hepatoprotective action against carbon tetrachloride induced hepatic damage in rats.
Rao
Guntupalli M Mohana
GM
Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow 226 001, Uttarpradesh, India. mmraos@rediffmail.com
Rao
Chandana V
CV
Pushpangadan
Palpu
P
Shirwaikar
Annie
A
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000880:Anthraquinones / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008107:Liver Diseases / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008315:Malondialdehyde / Q000378:metabolism; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D045470:Rubia / Q000737:chemistry*; D012838:Silymarin / Q000008:administration & dosage / Q000494:pharmacology
D000880:Anthraquinones; D000975:Antioxidants; D012838:Silymarin; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; C072500:rubiadin; D005982:Glutathione Transferase; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase; D005978:Glutathione