Production of highly concentrated xylitol by Candida magnoliae under a microaerobic condition maintained by simple fuzzy control.
Microbial production of xylitol from xylose was investigated using Candida magnoliae. In particular, the effect of the oxygenation condition on the xylitol production yield was examined and the significance of maintaining a microaerobic condition was demonstrated. A simple system of fuzzy logic control (FLC) was devised to maintain the microaerobic condition in the xylitol production phase by regulating the proportion of air (air flow rate) supplied to the fermentor. The input variables to the fuzzy control system were the dissolved oxygen (DO) concentration in the culture broth and the CO2 concentration in the exit gas. A batch cultivation test using the FLC system confirmed the importance of maintaining a constant microaerobic condition throughout the xylitol production phase, and indicated it would be advantageous for this phase to be prolonged. An intermittent fed-batch culture was therefore carried out. The FLC system allowed a constant microaerobic condition to be maintained, resulting in minimal cell mass production and constant xylitol accumulation in the culture medium. As a consequence, a very high xylitol concentration of 356 g/dm3 could be attained. The xylitol yield in the fed-batch culture was 0.75, which corresponded to 82% of the theoretical yield.
Nakano
K
K
Institute of Applied Biochemistry, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-0006, Japan.
Katsu
R
R
Tada
K
K
Matsumura
M
M