Repeated batch cultivation of Ralstonia eutropha for Poly (beta-hydroxybutyrate) production.
Batch cultivation of Ralstonia eutropha NRRL B14690 attained 21 g biomass l(-1) and 9.4 g poly(beta-hydroxybutyrate) l(-1) (0.45 g PHB g(-1 )dry wt(-1)) in 60 h. Repeated batch operation (empty-and-fill protocol) to remove 20% (v/v) of the culture broth and to supplement an equal volume of fresh media resulted in 49 g biomass l(-1) and 25 g PHB l(-1) (0.51 g PHB g(-1) dry wt(-1)) with an overall productivity of 0.42 g PHB l(-1 )h(-1 )in 67 h. In the two cycles of repeated batch fermentation there was a 3-fold increase in productivity as compared to batch.
Khanna
Shilpi
S
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, 110016, Hauz Khas, New Delhi, India.
Srivastava
Ashok Kumar
AK
D001431:Bacteriological Techniques / Q000379:methods; D018533:Biomass; D020118:Cupriavidus necator / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D006885:Hydroxybutyrates / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D013997:Time Factors; D000094122:Polyhydroxybutyrates
D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates