title: Gene expression profiling in rice young panicle and vegetative organs and identification of panicle-specific genes through known gene functions.
abstract: In rice, at the stage from pistil and stamen primordia formation to microsporocyte meiosis, the young panicle organs (YPO) make a great contribution to grain productivity. This period corresponds to the onset of meiosis and marks the transition from vegetative to reproductive stages. By comparing gene expression profiling of YPO with that of rice aerial vegetative organs (AVO), it is possible to gain further molecular insight into this period that is developmentally and functionally important. In this report, a total of 92,582 high-quality ESTs from 5'-end sequencing, including 44,247 from YPO and 48,335 from AVO, were obtained and classified. There were 12,884 (29.12%) ESTs from YPO and 16,304 (33.73%) ESTs from AVO matched to known genes, which generated 1,667 and 2,172 known genes, respectively, after integration of these ESTs. From the functions of known homologous genes, we identified some tissue- and developmental-stage-specified genes in YPO. The expression of these genes clearly reflected the unique functional characteristics of YPO. Furthermore, we estimated that there are about 10,000 mRNAs specifically expressed in rice YPO.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Jiabin
authors.initials: J
authors.lastname: Tang
authors.forename: Hong'ai
authors.initials: H
authors.lastname: Xia
authors.forename: Dayong
authors.initials: D
authors.lastname: Li
authors.forename: Mengliang
authors.initials: M
authors.lastname: Cao
authors.forename: Yong
authors.initials: Y
authors.lastname: Tao
authors.forename: Wei
authors.initials: W
authors.lastname: Tong
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Zhang
authors.forename: Songnian
authors.initials: S
authors.lastname: Hu
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Huanming
authors.initials: H
authors.lastname: Yang
authors.forename: Lihuang
authors.initials: L
authors.lastname: Zhu
mesh_terms: D017931:DNA Primers; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D017343:Genes, Plant; D008540:Meiosis; D012275:Oryza / Q000166:cytology / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger