Selection of a suitable strain from recombinant Escherichia coli strains with the same genetic structure expressing periplasmic hGM-CSF.
The selection of a suitable strain among five recombinant Escherichia coli strains with the same genetic structure that expresses the human granulocyte macrophage colony stimulating factor (hGM-CSF) was carried out based on four criteria:growth rate, expression level, plasmid stability and feasibility for protein extraction. There was no significant difference in growth between the five strains, while a suitable expression level, a high plasmid stability and a good feasibility for protein extraction from periplasmic space were observed for one of the recombinant strains. This strain expressed 27% hGM-CSF relative to total proteins and had 96% plasmid stability after 7-d subcultures on an antibiotic-free LB medium.
Fatemi
Seyed Safa-Ali
SS
Industrial Fermentation Group, National Research Center for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran.
Yakhchali
Bagher
B
Darian
Jafar Towfighi
JT
Shojaosadati
Seyed Abbas
SA
Zomorodipour
Ali Reza
AR
Karkhane
Ali Asghar
AA
Jazii
Ferdows Rastgar
FR