title: Analysis of polymorphism of 18S rRNA gene in Wuchereria bancrofti microfilariae.
abstract: The polymorphism of the 18S rRNA gene in Wuchereria bancrofti microfilariae (mf) collected from three different zones in India was analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The RFLPs of the amplified products obtained after digestion with restriction enzymes Ssp I, Msp I and Hha I showed no difference in the banding patterns among the mf isolates from different endemic zones. Further the sequencing of PCR products did not show any difference in the nucleotide sequence either. The phylogenetic analysis of the sequences of W. bancrofti mf isolates from different endemic zones has shown branching with the earlier reported sequences of W. bancrofti and its close relative Brugia malayi.
authors.affiliation: Department of Biochemistry & J.B. Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.
authors.forename: Yashodhar
authors.initials: Y
authors.lastname: Bhandari
authors.forename: Pankaj
authors.initials: P
authors.lastname: Dabir
authors.forename: Krithika
authors.initials: K
authors.lastname: Nandhakumar
authors.forename: Kannayakanahalli Maheshwarappa
authors.initials: KM
authors.lastname: Dayananda
authors.forename: Yogesh Shripad
authors.initials: YS
authors.lastname: Shouche
authors.forename: Maryada Venkata Rami
authors.initials: MV
authors.lastname: Reddy
mesh_terms: D000818:Animals; D017178:Brugia malayi / Q000235:genetics; D004247:DNA / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics*; D019003:Deoxyribonuclease HpaII / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D019353:Endemic Diseases; D005368:Filariasis / Q000469:parasitology; D017238:Genes, Helminth; D020459:Genes, rRNA; D006801:Humans; D007194:India; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014958:Wuchereria bancrofti / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D004247:DNA; D019003:Deoxyribonuclease HpaII; C061568:endodeoxyribonuclease SspI; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases