Effect of chromium on the fatty acid composition of two strains of Euglena gracilis.
The effect of hexavalent chromium on fatty acid composition was studied in two strains of Euglena gracilis; UTEX 753 (from the Culture Collection of Algae of Texas University, USA) and MAT (isolated from a highly polluted River). Both were grown in photoauxotrophic and photoheterotrophic conditions and exposed to two metal concentrations, one below and one above IC50. The high malondialdehyde (MDA) levels (3 to 7-fold) obtained with chromium concentration above IC50, suggested the existence of metal-induced lipid peroxidation. Total lipid content increased only with concentration below IC50, whereas it was inhibited by higher metal concentration. Photoheterotrophic control strains exhibited a significantly higher proportion of saturated and polyunsaturated fatty acids. Polyunsaturated acids were most affected by chromium, especially those related to chloroplast structures. Ultra-structure studies showed clear thylakoid disorganization in all treated cells. The results indicate that hexavalent chromium affects levels of fatty acids, especially those related to photosynthetic activity.
Rocchetta
Iara
I
Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Pab. II, Ciudad Universitaria, 1428 Buenos Aires, Argentina. rocchetta@bg.fcen.uba.ar
Mazzuca
Marcia
M
Conforti
Visitación
V
Ruiz
Laura
L
Balzaretti
Vilma
V
Ríos de Molina
María del Carmen
Mdel C
D000818:Animals; D002274:Carcinogens, Environmental / Q000494:pharmacology; D002736:Chloroplasts / Q000737:chemistry; D002857:Chromium / Q000494:pharmacology*; D003470:Culture Media; D005056:Euglena gracilis / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005227:Fatty Acids / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D008315:Malondialdehyde / Q000032:analysis; D008854:Microscopy, Electron / Q000379:methods; D045483:Rivers / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
D002274:Carcinogens, Environmental; D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D014874:Water Pollutants, Chemical; D002857:Chromium; C074702:chromium hexavalent ion; D008315:Malondialdehyde