title: In vitro and in vivo two-photon luminescence imaging of single gold nanorods.
abstract: Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos(4) dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.forename: Terry B
authors.initials: TB
authors.lastname: Huff
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Zweifel
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Philip S
authors.initials: PS
authors.lastname: Low
authors.forename: Alexander
authors.initials: A
authors.lastname: Wei
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001775:Blood Circulation; D001808:Blood Vessels; D003952:Diagnostic Imaging / Q000379:methods*; D004423:Ear / Q000098:blood supply; D005260:Female; D005454:Fluorescence Polarization; D006046:Gold; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal / Q000379:methods; D049329:Nanostructures
chemical_list: D006046:Gold