title: Active and passive immunization against oral Candida albicans infection in a murine model.
abstract: OBJECTIVE
  Clinical and laboratory studies are consistent with a major role for cell-mediated immunity in recovery from oral infection with Candida albicans, but the role of humoral immunity remains controversial. The purpose of this study was to establish the relative contributions of cellular and humoral immunity to protection against oral candidiasis in a murine model, and to determine whether host responses could be enhanced by different immunization strategies.
  
  
  RESULTS
  Active oral immunization was protective in BALB/c and CBA/CaH mice, reducing both fungal burden and duration of infection after secondary challenge, whereas systemic immunization failed to protect against subsequent oral challenge. Candida-specific IgM was the predominant antibody detected in serum following both primary and secondary oral challenge; however, Candida-specific salivary IgA was not detectable. Immunization by passive transfer of either lymphocytes or immune serum did not confer any significant protection against oral infection in either susceptible or resistant mouse strain.
  
  
  CONCLUSIONS
  The data demonstrate a possible role for mucosa-associated immunity following active immunization by the oral route, most likely exerted by local T lymphocytes resident in the oral mucosa, but there was no evidence to support a role for humoral immunity in protection against oral candidiasis.
authors.affiliation: Oral Biology and Pathology Research Unit, School of Dentistry, University of Queensland, Brisbane, Australia. c.farah@uq.edu.au
authors.forename: C S
authors.initials: CS
authors.lastname: Farah
authors.forename: R B
authors.initials: RB
authors.lastname: Ashman
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D000917:Antibody Formation / Q000276:immunology; D002176:Candida albicans / Q000276:immunology; D002180:Candidiasis, Oral / Q000517:prevention & control*; D004195:Disease Models, Animal; D004198:Disease Susceptibility / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology; D018928:Immunity, Mucosal / Q000276:immunology; D007116:Immunization, Passive; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D012463:Saliva / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination
chemical_list: D000908:Antibodies, Fungal; D007071:Immunoglobulin A, Secretory; D007075:Immunoglobulin M