title: Detection of human herpesviruses and polyomaviruses DNA in a group of patients with relapsing-remitting multiple sclerosis.
abstract: Multiple sclerosis (MS) is an inflammatory disease of the central nervous system whose pathological features consist of white matter plaques of primary demyelinization and loss of oligodendrocytes. Various risk factors have been associated with MS susceptibility. We have focused this study on different viruses. In particular in the present study we used PCR to search for the genomic DNA of HHV-1, HHV-2, HHV-8, BKV and JCV in urine and peripheral blood mononuclear cells (PBMC) samples from 44 relapsing-remitting MS (RRMS) patients. No viral DNA was found in any urine sample, whereas 29.5% of RRMS PBMC samples were positive. It is suggestive that Human herpesviruses (HHV-1 and HHV-8) were constantly present in all positive samples, indicating that viral agents could contribute to create the demyelination plaques and cause MS.
authors.affiliation: Department of Public Health Sciences, La Sapienza University, Rome, Italy. valeria.pietropaolo@uniroma1.it
authors.forename: Valeria
authors.initials: V
authors.lastname: Pietropaolo
authors.forename: Daniela
authors.initials: D
authors.lastname: Fioriti
authors.forename: Monica
authors.initials: M
authors.lastname: Mischitelli
authors.forename: Elena
authors.initials: E
authors.lastname: Anzivino
authors.forename: Manuela
authors.initials: M
authors.lastname: Santini
authors.forename: Enrico
authors.initials: E
authors.lastname: Millefiorini
authors.forename: Simone
authors.initials: S
authors.lastname: Di Rezze
authors.forename: Anna Marta
authors.initials: AM
authors.lastname: Degener
mesh_terms: D000293:Adolescent; D000328:Adult; D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D006566:Herpesviridae Infections / Q000150:complications; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000150:complications / Q000821:virology*; D016133:Polymerase Chain Reaction; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000150:complications; D018139:Simplexvirus / Q000235:genetics / Q000302:isolation & purification*; D014412:Tumor Virus Infections / Q000150:complications; D014556:Urine / Q000821:virology
chemical_list: D004279:DNA, Viral