Myocardial homing of nonmobilized peripheral-blood CD34+ cells after intracoronary injection.
Granulocyte--colony-stimulating factor administered for autologous hematopoietic stem cell isolation from blood may favor restenosis in patients implanted after acute myocardial infarction (AMI). We therefore tested the isolation of peripheral-blood CD34+ cells without mobilization in six patients with AMI. After large-volume cytapheresis and positive CD34+ cell selection, 3.6 to 27.6 million CD34+ cells were obtained. We performed intra-coronary implantation of these cells and recorded no restenosis or arrhythmia. We used positron emission tomography (PET) to assess myocardial-labeled CD34+ cell homing, which accounted for 5.5% of injected cells 1 hour after implantation. In conclusion, large amounts of CD34+ cells, in the range reported in previous studies, can be obtained from nonmobilized peripheral blood. PET with [18F]-fluorodeoxyglucose cell labeling is an efficient imaging method for homing assessment.
Blocklet
Didier
D
Department of Nuclear Medicine and PET/Biomedical Cyclotron Unit, Hôpital Universitaire Erasme, Université Libre de Bruxelles, Brussels, Belgium. dblockle@ulb.ac.be
Toungouz
Michel
M
Berkenboom
Guy
G
Lambermont
Micheline
M
Unger
Philippe
P
Preumont
Nicolas
N
Stoupel
Eric
E
Egrise
Dominique
D
Degaute
Jean-Paul
JP
Goldman
Michel
M
Goldman
Serge
S
D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism*; D002465:Cell Movement; D023903:Coronary Restenosis; D003331:Coronary Vessels / Q000502:physiology; D005240:Feasibility Studies; D005260:Female; D005500:Follow-Up Studies; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000628:therapy*; D009206:Myocardium / Q000648:ultrastructure*; D049268:Positron-Emission Tomography / Q000379:methods; D011877:Radionuclide Imaging / Q000379:methods
D018952:Antigens, CD34