[Protective effects of polarizing cardioplegia with Na(+) channel inhibitor in ex vivo rat heart preservation].
OBJECTIVE
To investigate the protective effects of polarizing cardioplegia with Na(+) channel inhibitor tetrodotoxin (TTX) in ex vivo rat heart preservation.


METHODS
Using a Langendorff preparation, Wistar rat hearts were arrested and preserved with St. Thomas (STH) solution (n=10) or with polarizing cardioplegia (TTX, n=10) for 7 h in hypothermic storage (10 degrees Celsius). All the hearts then underwent 30 min of reperfusion. Pre-ischemia and post-ischemia indexes of the rat hearts were determined, including the hemodynamic parameters, myocardial enzymology, ATP content and ultrastructural changes.


RESULTS
The recovery of hemodynamic parameters of the hearts in TTX group were better than those in STH group (P<0.01). In comparison with STH group, the leakage of creatine kinase (CK) and lactate dehydrogenase (LDH) in TTX group was significantly lower (P<0.05) and ATP level maintained a relative high level with better protected myocardial ultrastructure.


CONCLUSIONS
TTX polarizing cardioplegia provides more effective long-term hypothermic preservation of isolated rat hearts than STH.
Tan
Song-tao
ST
Department of Cardiothoracic Surgery, Guangdong Headquarter Hospital of Chinese People's Armed Police Force, Guangzhou 510507, China. Esongtaotan@sohu.com
Yang
Shuang-qiang
SQ
Yang
Chao-kun
CK
D000818:Animals; D002314:Cardioplegic Solutions; D006321:Heart / Q000187:drug effects*; D008297:Male; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D020011:Protective Agents; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride; D017923:Sodium-Hydrogen Exchangers / Q000037:antagonists & inhibitors*; D013779:Tetrodotoxin / Q000494:pharmacology*
D002314:Cardioplegic Solutions; D019188:Organ Preservation Solutions; D020011:Protective Agents; D017923:Sodium-Hydrogen Exchangers; D013779:Tetrodotoxin; D012965:Sodium Chloride