Development of protein, peptide, and small molecule catalysts using catalysis-based selection strategies.
We have developed peptide catalysts and antibody catalysts that catalyze aldol, retro-aldol, and Michael reactions via an enamine mechanism using reaction-based selections with 1,3-diketone derivatives. Nucleophilic amino groups of the catalysts were covalently trapped during the selections. We have also developed fluorogenic substrates that are useful for real-time monitoring of the progress of bond-forming reactions, such as aldol reactions, by an increase in fluorescence. These fluorogenic substrates have been used to monitor peptide-catalyzed, antibody-catalyzed, enzyme-catalyzed, and small molecule-catalyzed reactions. Catalysis-based screening using fluorogenic substrates will accelerate rapid identification of superior catalysts and reaction conditions.
Tanaka
Fujie
F
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. ftanaka@scripps.edu
D000446:Aldehyde-Lyases / Q000737:chemistry; D000447:Aldehydes / Q000737:chemistry; D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002384:Catalysis; D005456:Fluorescent Dyes / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
D000447:Aldehydes; D000906:Antibodies; D005456:Fluorescent Dyes; D010455:Peptides; D011506:Proteins; D012997:Solvents; D002244:Carbon; D000446:Aldehyde-Lyases