title: Low-volume amplification on chemically structured chips using the PowerPlex16 DNA amplification kit.
abstract: In forensic DNA analysis, improvement of DNA typing technologies has always been an issue. It has been shown that DNA amplification in low volumes is a suitable way to enhance the sensitivity and efficiency of amplification. In this study, DNA amplification was performed on a flat, chemically structured glass slide in 1-microl reaction volumes from cell line DNA contents between 1,000 and 4 pg. On-chip DNA amplification reproducibly yielded full allelic profiles from as little as 32 pg of template DNA. Applicability on the simultaneous amplification of 15 short tandem repeats and of a segment of the Amelogenin gene, which are routinely used in forensic DNA analysis, is shown. The results are compared to conventional in-tube amplification carried out in 25-microl reaction volumes.
authors.affiliation: Institute of Legal Medicine, Albert Ludwig University Freiburg, Albertstrasse 9, 79104, Freiburg, Germany. ulrike.schmidt@uniklinik-freiburg.de
authors.forename: Ulrike
authors.initials: U
authors.lastname: Schmidt
authors.forename: Sabine
authors.initials: S
authors.lastname: Lutz-Bonengel
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Weisser
authors.forename: Timo
authors.initials: T
authors.lastname: Sänger
authors.forename: Stefan
authors.initials: S
authors.lastname: Pollak
authors.forename: Ulrike
authors.initials: U
authors.lastname: Schön
authors.forename: Thomas
authors.initials: T
authors.lastname: Zacher
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Mann
mesh_terms: D016022:Case-Control Studies; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000295:instrumentation*; D004591:Electrophoresis, Polyacrylamide Gel; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods
chemical_list: D004247:DNA