Neocortical kindling is associated with opposing alterations in dendritic morphology in neocortical layer V and striatum from neocortical layer III.
Previous research has shown that seizures kindled in the corpus callosum result in a persistent enhancement of the callosal-neocortical evoked response but only a transient reduction in layer III pyramidal cell morphology. To date, there are no reports on the direct effects of repeated seizures on dendritic morphology in layer V, the pyramidal layer thought to mediate the kindling-induced enhanced evoked response. This experiment examined the effect of repeated seizures elicited from the corpus callosum, at the level of the frontal neocortex, on the morphology of sensorimotor frontal (Fr1) and occipital (OC1) neocortical layer V, as well as striatal and neuronal dendrites, in male rats. After 25 days of electrically elicited seizures or handling control, rats were sacrificed either 2 days or 3 weeks following the last seizure and processed for Golgi-Cox staining. Analysis of the impregnated pyramidal cell dendrites indicated a significant increase in the amount of dendritic length and branching in rats 2 days, but a decrease 3 weeks, following the last seizure. There was no effect at the distant occipital site. The differential effect between layer V pyramidal neurons and layer III pyramidal neurons suggests that these areas play different roles in the expression of seizures and the adaptation of the brain to the persistent effect of kindling.
Teskey
G Campbell
GC
Behavioural Neuroscience Research Group, Department of Psychology, University of Calgary, Alberta, Canada. gteskey@ucalgary.ca
Monfils
Marie-H
MH
Silasi
Greg
G
Kolb
Bryan
B
D000818:Animals; D048429:Cell Size; D003342:Corpus Striatum / Q000503:physiopathology; D003712:Dendrites / Q000473:pathology* / Q000502:physiology / Q000648:ultrastructure; D004558:Electric Stimulation / Q000009:adverse effects; D004827:Epilepsy / Q000473:pathology* / Q000503:physiopathology; D007696:Kindling, Neurologic / Q000473:pathology* / Q000502:physiology; D008297:Male; D019579:Neocortex / Q000473:pathology* / Q000503:physiopathology; D017966:Pyramidal Cells / Q000473:pathology* / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D020318:Rats, Long-Evans; D016622:Silver Staining / Q000379:methods; D013997:Time Factors