title: Rapid selection of nonhotspot mutants among hisD+ revertants of Salmonella typhimurium TA98 in Ames test by peptide nucleic acid (PNA)-mediated PCR clamping.
abstract: Ames test is the most popular method of assessing mutagenicity using Salmonella typhimurium as an indicator. Recently, sequence analyses have been introduced for the investigation of mutation mechanisms. Most revertants (>70%) carry 2-bp deletion within an 8-bp CG repeat in hisD (hotspot mutation) in the Ames test using S. typhimurium TA98. We developed a new specific amplification method for nonhotspot mutants by peptide nucleic acid (PNA)-mediated PCR clamping. It markedly reduces the labor and cost of this kind of studies.
authors.affiliation: United Graduate School of Agricultural Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takiya
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Horie
authors.forename: Satoshi
authors.initials: S
authors.lastname: Futo
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kawai
authors.forename: Tohru
authors.initials: T
authors.lastname: Suzuki