Antisense-induced blockade of GATA-3 expression could inhibit Th2 excursion of tumor cells in vitro and in vivo.
Previous studies have shown that tumor cells predominantly express Th2 type cytokines and transcription factors. GATA-3, as a Th2-specific transcription factor, plays a central role in positive-regulating Th2 development. So whether the expression of GATA-3 in tumor cells has any effect on tumor development is a question of interest. In the present study, we inhibited the expression of GATA-3 in tumor cells through antisense RNA blockade technique, and observed its effects on tumor in vitro and in vivo. Our results showed that antisense GATA-3 treatment could inhibit the expression of TNF-alpha and Th2 cytokines in tumor cells, and antisense-induced blockade of GATA-3 could also depress tumor growth in tumor-bearing mice. We suggest that the ratio of T-bet/GATA-3 can be evaluated as a more important marker of the status of Th1/Th2 type. And our results might provide some evidence about the molecular regulatory mechanisms in tumor cell development.
Yao
Dongzhu
D
Institute of Immunology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
Zhang
Xiaojun
X
Wei
Haiming
H
Tian
Zhigang
Z
D000818:Animals; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D016207:Cytokines / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000096:biosynthesis / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009369:Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D050990:GATA3 Transcription Factor; D016372:RNA, Antisense; D014409:Tumor Necrosis Factor-alpha