Determination of four active saponins of Panax notoginseng in rat feces by high-performance liquid chromatography.
A method is developed for the determination of ginsenoside Rg1, Rb1, Rd, and notoginsenoside R1 of Panax notoginseng (PNS) in rat feces after oral and intravenous administration of total saponins of PNS. The fecal samples are treated with organic extraction and solid-phase extraction prior to high-performance liquid chromatography. The calibration curves for the four saponins are linear in the given concentration ranges. The precision of the method is in the range of 1.0-10.0% (relative standard deviation), and the accuracy is between 80.0% and 110%. The recoveries of this method are all over 75%. This method is successfully applied to the analyses of fecal samples of rats treated with PNS.
Li
Lie
L
School of Pharmaceutical Sciences and Modern Research Center for Traditional Chinese Medicine, Peking University, Beijing 100083, P.R. China.
Sheng
Yuxin
Y
Zhang
Jinlan
J
Guo
Dean
D
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry*; D005894:Panax / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D012680:Sensitivity and Specificity
D012503:Saponins