title: Complete cDNA sequence of chitin deacetylase from Gongronella butleri and its phylogenetic analysis revealed clusters corresponding to taxonomic classification of fungi.
abstract: A cDNA library containing a chitin deacetylase (CDA) gene from a zygomycete Gongronella butleri was constructed and the complete gene was sequenced. The complete gene contains an open reading frame of 1290 nucleotides which encodes a sequence of 430 amino acid residues. The gene sequence consists of nucleotides encoding a polysaccharide deacetylase domain located in the middle, covering 34% of the entire sequence. This domain shares high sequence similarities with CDAs of fungi and other deacetylases including the nodb-like protein from Sinorhizobium meliloti and Bacillus subtilis, xylanase D from Cellulomonas fimi, and acetylxylan esterase A from Streptomyces lividans. The deduced amino acid sequence shows homology with CDAs from Mucor rouxii (48% identity) and Phycomyces blakesleeanus (30% identity). Phylogenetic analysis reveals that CDAs are clustered in accordance with the taxonomic classification of the fungi.
authors.affiliation: Department of Biological Sciences, National University of Singapore, Lower Kent Ridge Road, Singapore 117543, Singapore.
authors.forename: Theingi
authors.initials: T
authors.lastname: Maw
authors.forename: Teck Koon
authors.initials: TK
authors.lastname: Tan
authors.forename: Eugene
authors.initials: E
authors.lastname: Khor
authors.forename: Sek Man
authors.initials: SM
authors.lastname: Wong