Characterization of murine monoclonal antibodies against Helicobacter pylori lipopolysaccharide specific for Lex and Ley blood group determinants.
The cell envelope of Helicobacter pylori contains lipopolysaccharide (LPS), the O-chain of which expresses type 2 Lex and Ley blood group antigens, which mimic human gastric mucosal cell-surface glycoconjugates and may contribute to the survival of H. pylori in gastric mucosa. Here we describe the generation of monoclonal antibodies specific for Lex and Ley blood group determinants and the characterization of their binding properties using purified, structurally defined H. pylori LPS, synthetic glycoconjugates, and H. pylori cells. Analysis of oligosaccharide binding by SPR provided a rapid and reliable means for characterization of antibody affinities. One of the antibodies, anti-Lex, was of IgG3 subclass and had superior binding characteristics as compared with the commercially available anti-Lex IgM. These antibodies could have potential in the immunodiagnosis of certain types of cancer, in serotyping of H. pylori isolates, and in structure-function studies.
Altman
Eleonora
E
Institute for Biological Sciences, NationalResearch Council of Canada, Ottawa, ON K1A 0R6, Canada. Eleonora.altman@nrc-cnrc.gc.ca
Harrison
Blair A
BA
Hirama
Tomoko
T
Chandan
Vandana
V
To
Rebecca
R
MacKenzie
Roger
R
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D001483:Base Sequence; D002240:Carbohydrate Sequence; D018076:DNA, Complementary; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D020349:Surface Plasmon Resonance
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D007983:Lewis Blood Group Antigens; D008070:Lipopolysaccharides