title: Mutation in IRO1 tightly linked with URA3 gene reduces virulence of Candida albicans.
abstract: Gene deletion in the pathogenic fungus Candida albicans has relied heavily on a URA3 cassette and a recipient delta ura3 strain CAI4. The IRO1 gene adjacent to URA3 was inadvertently deleted during construction of CAI4. We report here that a mutation in IRO1 reduces virulence of C. albicans.
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan. chibana@faculty.chiba-u.jp
authors.forename: Hiroji
authors.initials: H
authors.lastname: Chibana
authors.forename: Jun
authors.initials: J
authors.lastname: Uno
authors.forename: Tamaki
authors.initials: T
authors.lastname: Cho
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Mikami
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000254:growth & development / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology; D004195:Disease Models, Animal; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D017353:Gene Deletion; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D016019:Survival Analysis; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D014774:Virulence / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; C430663:IRO1 protein, Candida albicans; D014157:Transcription Factors