Lignans and secoiridoids from the root bark of Chionanthus virginicus L.: isolation, identification and HPLC analysis.
Five lignans, phillyrin (1), pinoresinol-beta-D-glucoside (2), pinoresinol di-beta-D-glucoside (3), phillyrin-2-O-beta-D-glucoside (6), phillyrin-6-O-beta-D-glucoside (7), and three secoiridoids, oleuropein (4), ligustroside (5) and angustifolioside B (8), have been isolated from the root bark of Chionanthus virginicus L. (Oleaceae), a raw material used in the commercial preparation of homeopathy tinctures. Compound 6 is a new lignan, and compounds 2, 3, 4, 5, 7 and 8 are described for the first time in the title plant. The structures of these compounds were elucidated by spectroscopic methods (NMR and HPLC-MS). An isocratic HPLC method for the rapid characterisation and quantification of the major constituents, 1 and 4, of the root bark is described and validated.
Boyer
Laurent
L
Laboratoire de Pharmacognosie, Faculté de Pharmacie, Marseille, France.
Elias
Riad
R
Taoubi
Khalil
K
Debrauwer
Laurent
L
Faure
Robert
R
Baghdikian
Béatrice
B
Balansard
Guy
G
D002851:Chromatography, High Pressure Liquid; D039823:Iridoids / Q000032:analysis* / Q000737:chemistry; D017705:Lignans / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D029592:Oleaceae / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results
D039823:Iridoids; D017705:Lignans