Creation of a new synthetic medium for culturing Helicobacter pylori.
We developed a scheme of consecutive replacement of complex components of a known Brucella medium containing peptones and blood with simple analogs and created a synthetic medium for Helicobacter pylori culturing. H. pylori cells require hemic iron for their growth; an appreciable increment in biomass was ensured by hemoglobin, but not simpler hemocontaining compounds (hemin and cytochrome C). Glutamine (20 g/liter) was used as the main nitrogen-containing component, and other amino acids were added in trace amounts. Adhesion was provided by adding agarose gel (0.1%) also promoting the increase in biomass. The proposed medium of a certain chemical composition differs from the known foreign analogs by the presence of hemocontaining component (hemoglobin), short period of exponential growth, and appreciable accumulation of cell protein.
Vartanova
N O
NO
II Mechnikov Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow. nune4me@mail.ru
Arzumanyan
V G
VG
Serdyuk
O A
OA
Temper
R M
RM
D000818:Animals; D001431:Bacteriological Techniques; D001769:Blood / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D045304:Cytochromes c / Q000378:metabolism; D016480:Helicobacter pylori / Q000166:cytology / Q000378:metabolism*; D006427:Hemin / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans
D003470:Culture Media; D006454:Hemoglobins; D006427:Hemin; D045304:Cytochromes c