title: Accumulation of L-cystathionine by an Escherichia coli mutant deficient in cystathionine beta-lyase.
abstract: An Escherichia coli mutant deficient in cystathionine beta-lyase was found to accumulate a substance detectable by ninhydrin reaction and chloride platinic acid reaction in its cells (rarely in the culture supernatant) when cultured with a limited amount (50-200 microg/ml) of L-methionine to support the growth. The product was released by freezing treatment and isolated by ion-exchange chromatography (cation exchange resin: Daiaion SK1B). It was identified as L-cystathionine by liquid chromatography-mass spectrometry, 13C- and 1H-nuclear magnetic resonance analyses and high-performance liquid chromatography (as its 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate derivative).
authors.affiliation: Graduate School of Integrated Science and Art, University of East Asia, 2-1 Ichinomiya-gakuenmachi, Shimonoseki City, Yamaguchi 751-0807, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Nishi
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Kazumi
authors.initials: K
authors.lastname: Araki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Furihata
authors.forename: Masayuki
authors.initials: M
authors.lastname: Onodera
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Toda