title: The inhibitory effects of deltamethrin on dopamine biosynthesis in rat PC12 cells.
abstract: The inhibitory effects of deltamethrin (DM) on dopamine biosynthesis in PC12 cells were investigated. Levels of dopamine in PC12 cells were detected after treatment with different doses from 10(-4)M to 10(-6)M deltamethrin or combined with 20 microM or 50 microM l-3,4-dihydroxyphenylalanine (L-Dopa) for 3 h or 12 h by HPLC. Flow cytometry (FCM) and RT-PCR technology were used to detect the levels of tyrosine hydroxylase (TH) mRNA and protein in PC12 cells treated with different doses of deltamethrin for 3 h or 12 h. Deltamethrin decreased the levels of dopamine, TH mRNA and TH protein in PC12 cells, and the increased dopamine levels induced by l-Dopa at 20-50 microM were also decreased by deltamethrin for 3 h or 12 h. These results indicate that deltamethrin decreases dopamine content by the inhibition of TH mRNA and protein level in PC12 cells and maybe also have inhibitory effect on aromatic l-amino acid decarboxylase.
authors.affiliation: Department of Toxicology, MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College of Huazhong Science and Technology University, Wuhan 430030, China.
authors.forename: Gong-Ping
authors.initials: GP
authors.lastname: Liu
authors.forename: Nian
authors.initials: N
authors.lastname: Shi
mesh_terms: D000818:Animals; D004298:Dopamine / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004791:Enzyme Inhibitors / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity*; D007980:Levodopa / Q000494:pharmacology; D009570:Nitriles / Q000633:toxicity*; D016716:PC12 Cells / Q000187:drug effects* / Q000378:metabolism; D011722:Pyrethrins / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: D004338:Drug Combinations; D004791:Enzyme Inhibitors; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D012333:RNA, Messenger; C017180:decamethrin; D007980:Levodopa; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine