title: Effects of difructose anhydride III (DFA III) administration on rat intestinal microbiota.
abstract: The effects of difructose anhydride III (di-D-fructofuranose-1,2':2,3'-dianhydride; DFA III) administration (3% DFA III for 4 weeks) on rat intestinal microbiota were examined using denaturing gradient gel electrophoresis (DGGE). According to DGGE profiles, the number of bacteria related to Bacteroides acidofaciens and uncultured bacteria within the Clostridium lituseburense group decreased, while that of bacteria related to Bacteroides vulgatus, Bacteroides uniformis and Ruminococcus productus increased in DFA III-fed rat cecum. In the cecal contents of DFA III-fed rats, a lowering of pH and an increase in short chain fatty acids (SCFAs), especially acetic acid, were observed. The DFA III-assimilating bacterium, Ruminococcus sp. M-1, was isolated from the cecal contents of DFA III-fed rats. The strain had 98% similarity with R. productus ATCC 27340T (L76595), and mainly produced acetic acid. These results confirmed that the bacteria harmful to host health were not increased by DFA III administration. Moreover, DFA III stimulated the growth of Ruminococcus sp. M-1 producing acetic acid, which may alter the intestinal microbiota towards a healthier composition. It is expected that DFA III would be a new candidate as a prebiotic.
authors.affiliation: Northern Advancement Center for Science and Technology, Nishi 12, Kita 21, Kita-ku, Sapporo, Hokkaido 001-0021, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Minamida
authors.forename: Kazuki
authors.initials: K
authors.lastname: Shiga
authors.forename: I Nengah
authors.initials: IN
authors.lastname: Sujaya
authors.forename: Teruo
authors.initials: T
authors.lastname: Sone
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yokota
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hara
authors.forename: Kozo
authors.initials: K
authors.lastname: Asano
authors.forename: Fusao
authors.initials: F
authors.lastname: Tomita
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001439:Bacteroides / Q000187:drug effects* / Q000302:isolation & purification; D002432:Cecum / Q000187:drug effects* / Q000382:microbiology*; D003013:Clostridium / Q000187:drug effects*; D019587:Dietary Supplements / Q000382:microbiology; D004187:Disaccharides / Q000008:administration & dosage*; D005766:Gastrointestinal Contents / Q000382:microbiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D045857:Ruminococcus / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D004187:Disaccharides; C087758:difructose anhydride III