title: [Regulatory volume decrease and its mechanism in nasopharyngeal epithelial cells].
abstract: To investigate regulatory volume decrease (RVD) and its mechanism in primary-culturing fetal human nasopharyngeal epithelial cells, living cell imaging technique was employed to detect the volume changes following exposure to hypotonic solution, and blockage of Cl- channels was used to clarify the role of Cl- channels in RVD. The results showed that extracellular hypotonic treatment swelled the cells and induced RVD. 47% hypotonic solution (160 mOsmol/L) swelled the cell by 144.7% and induced 38.7% recovery of cell volume within 20 min. RVD was correlated negatively to the extracellular osmolarity (r=-0.99, P<0.05) and positively to the swelling volume(r=0.99, P<0.05) in "S" shape, respectively. Chloride channel blockers, tamoxfen (20 micromol/ L), ATP (10 mmol/L) and NPPB (100 micromol/L), inhibited RVD by 100%, 76.3% and 62.7% (P< 0.01), respectively. The results indicated that primary-culturing fetal human nasopharyngeal epithelial cells are capable of RVD. Cl- efflux through Cl- channels is the key mechanism of RVD.
authors.affiliation: Laboratory of Physiology, Guangdong Medical College, Zhanjiang.
authors.forename: Xue Rong
authors.initials: XR
authors.lastname: Sun
authors.forename: Li Xin
authors.initials: LX
authors.lastname: Chen
authors.forename: Jian Wen
authors.initials: JW
authors.lastname: Mao
authors.forename: Lin Yan
authors.initials: LY
authors.lastname: Zhu
authors.forename: Si Huai
authors.initials: SH
authors.lastname: Nie
authors.forename: Ping
authors.initials: P
authors.lastname: Zhong
authors.forename: Pan
authors.initials: P
authors.lastname: Li
authors.forename: Li Wei
authors.initials: LW
authors.lastname: Wang
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D048429:Cell Size / Q000187:drug effects*; D002478:Cells, Cultured; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology; D009305:Nasopharynx / Q000166:cytology*; D009579:Nitrobenzoates / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology
chemical_list: D018118:Chloride Channels; D007038:Hypotonic Solutions; D009579:Nitrobenzoates; D013629:Tamoxifen; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D000255:Adenosine Triphosphate