title: Cytochrome P4501A induction and DNA adduct formation in glaucous gulls (Larus hyperboreus), fed with environmentally contaminated gull eggs.
abstract: This study indicates that complex mixtures of pollutants found in the Arctic marine environment have genotoxic effects in glaucous gulls (Larus hyperboreus). DNA adducts were quantified, by the (32)P-postlabeling technique, in liver samples from gulls fed with hen eggs (controls) and from gulls fed with environmentally contaminated gull eggs (exposed). All birds were grown and fed under laboratory conditions. Hepatic homologues to mammalian cytochrome P4501A (CYP1A) proteins were also determined by Western blotting. DNA adducts were detected in all but one liver sample, but the exposed birds had a significantly increased level of DNA adducts relative to that of the controls. There was no clear significant correlation between the DNA adduct level and the level of organochlorine compounds (OCs) in blood. The level of CYP1A protein was significantly higher in the liver of exposed male gulls than in the liver of control males and positively correlated, with significance, to the level of OC compounds measured in blood. There was no significant correlation between the level of DNA adducts and the CYP1A protein content.
authors.affiliation: Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway. Lene.Ostby@bio.ntnu.no
authors.forename: Lene
authors.initials: L
authors.lastname: Østby
authors.forename: Geir Wing
authors.initials: GW
authors.lastname: Gabrielsen
authors.forename: Ase
authors.initials: A
authors.lastname: Krøkje
mesh_terms: D000818:Animals; D015415:Biomarkers; D046091:Charadriiformes; D002645:Chickens; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D018736:DNA Adducts; D004531:Eggs / Q000032:analysis; D004785:Environmental Pollutants / Q000097:blood / Q000633:toxicity*; D005260:Female; D006843:Hydrocarbons, Chlorinated / Q000097:blood / Q000633:toxicity*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D012737:Sex Factors
chemical_list: D015415:Biomarkers; D018736:DNA Adducts; D004785:Environmental Pollutants; D006843:Hydrocarbons, Chlorinated; D019363:Cytochrome P-450 CYP1A1