title: Proteomic dataset of mouse aortic smooth muscle cells.
abstract: In an accompanying study (in this issue, DOI 10.1002/pmic.200402044), we have characterised the proteome of Sca-1(+) progenitor cells, which may function as precursors of vascular smooth muscle cells (SMCs). In the present study, we have analysed and mapped protein expression in aortic SMCs of mice, using 2-DE, MALDI-TOF MS and MS/MS. The 2-D system comprised a non-linear immobilised pH 3-10 gradient in the first dimension (separating proteins with pI values of pH 3-10), and 12%T SDS-PAGE in the second dimension (separating proteins in the range 15,000-150,000 Da). Of the 2400 spots visualised, a subset of 267 protein spots was analysed, with 235 protein spots being identified corresponding to 154 unique proteins. The data presented here are the first map of aortic SMCs and the most extensive analysis of SMC proteins published so far. This valuable tool should provide a basis for comparative studies of protein expression in vascular smooth muscle of transgenic mice and is available on our website hhtp://www.vascular-proteomics.com.
authors.affiliation: Department of Cardiac and Vascular Sciences, St George's, University of London, UK. mlmayr@sgul.ac.uk
authors.forename: Ursula
authors.initials: U
authors.lastname: Mayr
authors.forename: Manuel
authors.initials: M
authors.lastname: Mayr
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Yin
authors.forename: Shajna
authors.initials: S
authors.lastname: Begum
authors.forename: Edward
authors.initials: E
authors.lastname: Tarelli
authors.forename: Robin
authors.initials: R
authors.lastname: Wait
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Xu
mesh_terms: D000818:Animals; D001011:Aorta / Q000737:chemistry*; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry; D051379:Mice; D009124:Muscle Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D009131:Muscle, Smooth, Vascular / Q000737:chemistry*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004798:Enzymes; D009124:Muscle Proteins