title: Rapid multiplex single nucleotide polymorphism genotyping based on single base extension reactions and color-coded beads.
abstract: A single nucleotide polymorphism (SNP) typing method using color-coded beads is promising because it is easy to use and inexpensive. However, the present protocols are not suitable for clinical and diagnostic applications because they need centrifugation for bead-washing. Here, we developed a simplified protocol without a bead-washing procedure that enables SNP typing of PCR amplified fragments in only 30 min.
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-0012, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Fujimura
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kohara
authors.forename: Kazunori
authors.initials: K
authors.lastname: Okano
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yohda
authors.forename: Hideki
authors.initials: H
authors.lastname: Kambara