Pokemon expression in malignant glioma: an application of bioinformatics methods.
OBJECTIVE
In this report the authors review the role of bioinformatics in the design of a research project in which the molecular genetics of malignant glioma were studied. A project to characterize Pokemon expression in malignant glioma was developed, refined, and implemented using bioinformatics methods.


METHODS
Using the resources available from the National Center for Biotechnology Information, the messenger RNA (mRNA) sequence for Pokemon was determined. With this information and online primer design tools, novel primers were designed that would specifically amplify Pokemon mRNA by using reverse transcription-polymerase chain reaction assays.


CONCLUSIONS
The promise of bioinformatics is in the rapid and widespread dissemination and analysis of genomic information. This information is then used in research investigating the genetic basis of disease. In this paper the authors review the bioinformatics methods used in their study of Pokemon expression in malignant glioma.
Rovin
Richard A
RA
Marquette General Hospital, Department of Biology, Northern Michigan University, Marquette, Michigan, USA. rarovin@penmed.com
Winn
Robert
R
D001483:Base Sequence / Q000235:genetics; D001932:Brain Neoplasms / Q000175:diagnosis / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D019295:Computational Biology / Q000379:methods* / Q000639:trends; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D030561:Databases, Nucleic Acid / Q000639:trends*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D020868:Gene Silencing / Q000502:physiology; D005819:Genetic Markers / Q000235:genetics*; D015698:Genomic Library; D005910:Glioma / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012097:Repressor Proteins / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics
D017931:DNA Primers; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D025803:Tumor Suppressor Protein p14ARF; C117093:Zbtb7a protein, mouse