title: Minimally invasive method for murine brain fixation.
abstract: Complete brain fixation can be achieved with transthoracic cardiac infusion without thoracotomy. Light and electron microscopy tissue sections reveal preservation of cytoplasmic and nuclear structure at all magnification levels. Punched samples were obtained from the fixed tissue specimens in precisely localized areas for study using electron microscopy. This perfusion fixation technique provides both faster tissue harvesting capability and higher quality tissue preservation, without the artifacts of brain swelling and ventricular dilation observed in direct cardiac perfusion. Acute, discrete change in brain tissue can be studied.
authors.affiliation: New York University School of Medicine, New York, NY, USA.
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Eichenbaum
authors.forename: Joseph W
authors.initials: JW
authors.lastname: Eichenbaum
authors.forename: Ahmed
authors.initials: A
authors.lastname: Fadiel
authors.forename: Douglas C
authors.initials: DC
authors.lastname: Miller
authors.forename: Necdet
authors.initials: N
authors.lastname: Demir
authors.forename: Frederick
authors.initials: F
authors.lastname: Naftolin
authors.forename: Arnold
authors.initials: A
authors.lastname: Stern
authors.forename: Paul H
authors.initials: PH
authors.lastname: Pevsner
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D003593:Cytoplasm / Q000378:metabolism; D005404:Fixatives; D006652:Histological Techniques; D051379:Mice; D008854:Microscopy, Electron; D008867:Microtomy; D009474:Neurons / Q000378:metabolism; D010477:Perfusion; D011309:Preservation, Biological; D016610:Tissue Embedding / Q000379:methods*; D014021:Tissue Preservation / Q000295:instrumentation* / Q000379:methods*
chemical_list: D005404:Fixatives