title: Imaging and spatial distribution of beta-amyloid peptide and metal ions in Alzheimer's plaques by laser ablation-inductively coupled plasma-mass spectrometry.
abstract: Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) has been developed as a new strategy for detection and imaging of beta-amyloid protein in immunohistochemical sections from the brains of a transgenic mouse model of Alzheimer's disease. The distribution of beta-amyloid deposits in tissue was based on measurement of Eu- and Ni-coupled antibodies. The laser-based methodologies (spot ablation, single line raster, and two-dimensional imaging) were also used to detect and map trace element distributions and thus provide a novel probe for both elemental and protein data. We also report the combination of laser capture microdissection with LA-ICP-MS as an alternative strategy for microanalysis of immunohistochemical sections.
authors.affiliation: Centre for Analytical Sciences, The University of Sheffield, Sheffield, UK.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Hutchinson
authors.forename: Alan G
authors.initials: AG
authors.lastname: Cox
authors.forename: Cameron W
authors.initials: CW
authors.lastname: McLeod
authors.forename: Peter S
authors.initials: PS
authors.lastname: Marshall
authors.forename: Alex
authors.initials: A
authors.lastname: Harper
authors.forename: Emma L
authors.initials: EL
authors.lastname: Dawson
authors.forename: David R
authors.initials: DR
authors.lastname: Howlett
mesh_terms: D000544:Alzheimer Disease / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000276:immunology; D000818:Animals; D001921:Brain / Q000473:pathology; D001923:Brain Chemistry; D005063:Europium / Q000032:analysis*; D007554:Isotopes; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D009532:Nickel / Q000032:analysis*; D058225:Plaque, Amyloid / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D007554:Isotopes; D005063:Europium; D009532:Nickel