title: [PCR products with heterozygous mutations containing two types of heteroduplexes].
abstract: To confirm that PCR products with heterozygous mutations contain not only wide-type and mutant homoduplexes, but also two types of heteroduplexes.
  
  
  
  An insertion-deletion mutation in the exon 1 of KRT9 gene (497delAinsGGCT), which caused Chinese epidermolytic palmoplantar keratoderma (EPPK) was investigated by polymerase chain reaction (PCR), polyacrylamide gel electrophoresis (PAGE) and denaturing high-performance liquid chromatography(DHPLC).
  
  
  
  Two heteroduplexes and two homoduplexes in the PCR product from the heterozygous mutation of the exon 1 of KRT9 (497delAinsGGCT) were detected.
  
  
  
  PCR products from KRT9 gene with heterozygous mutations contain two types of heteroduplexes. It is without the need to perform heating and cooling PCR products obtained from heterozygous mutations in advance before the mutation screening steps such as denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), conformation-sensitive gel electrophoresis (CSGE), DHPLC and heteroduplex analysis (HA), etc.
authors.affiliation: Department of Medical Genetics, Institute of Cell Biology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
authors.forename: Xian-ning
authors.initials: XN
authors.lastname: Zhang
authors.forename: Xin-hui
authors.initials: XH
authors.lastname: He
authors.forename: Ji-cheng
authors.initials: JC
authors.lastname: Li
mesh_terms: D020137:Base Pair Mismatch; D002851:Chromatography, High Pressure Liquid; D004252:DNA Mutational Analysis; D004591:Electrophoresis, Polyacrylamide Gel; D020180:Heteroduplex Analysis; D006579:Heterozygote; D006801:Humans; D053534:Keratin-9; D007633:Keratins / Q000235:genetics*; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes; D016133:Polymerase Chain Reaction
chemical_list: C507440:KRT9 protein, human; D053534:Keratin-9; D009692:Nucleic Acid Heteroduplexes; D007633:Keratins