Transition of mechanical property of porous alginate scaffold with cells during culture period.
The rupture forces of porous alginate scaffolds seeded with hepatocytes or fibroblast-like cells increased during 3 d of culture and then decreased. The phenomenon was independent of the number of viable cells within the scaffolds, but dependent on protein adsorption to the alginate as well as a reduction in the degree of crosslinks of the calcium-alginate gel.
Sakai
Shinji
S
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. sakai@chem-eng.kyushu-u.ac.jp
Masuhara
Hiroki
H
Yamada
Yusuke
Y
Ono
Tsutomu
T
Ijima
Hiroyuki
H
Kawakami
Koei
K
D000464:Alginates / Q000032:analysis / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D019245:Compressive Strength / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology*; D020723:Glucuronic Acid / Q000032:analysis / Q000737:chemistry; D006603:Hexuronic Acids / Q000032:analysis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D051379:Mice; D016062:Porosity; D013314:Stress, Mechanical; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020723:Glucuronic Acid