title: Evaluation of phosphate removal from water by immobilized phosphate-binding protein PstS.
abstract: The phosphate (P(i))-binding protein PstS is a member of a family of periplasmic proteins that act as high-affinity receptors for active transport systems in bacteria. PstS protein purified from Pseudomonas aeruginosa was immobilized to N-hydroxysuccinimide-activated Sepharose, packed into a plastic column (5 x 70 mm), and examined for its potential ability to remove P(i) from water. The PstS-Sepharose column completely removed P(i) from 32P-labeled pond water containing about 0.5 microM P(i) (0.015 mg P per liter). More than 90% of 32P-P(i) that was retained in the column could be eluted by washing with low-pH water.
authors.affiliation: Department of Molecular Biotechnology, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8527, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kuroda
authors.forename: H
authors.initials: H
authors.lastname: Kunimoto
authors.forename: T
authors.initials: T
authors.lastname: Morohoshi
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: J
authors.initials: J
authors.lastname: Kato
authors.forename: N
authors.initials: N
authors.lastname: Takiguchi
authors.forename: A
authors.initials: A
authors.lastname: Miya
authors.forename: H
authors.initials: H
authors.lastname: Ohtake