A laboratory demonstration for learning about mast cell degranulation.
A simple experimental model of cell degranulation was implemented that exposed mast cells obtained from Sprague-Dawley rats to saponin. The model is flexible, asy, and low cost, is not very time-consuming to run, and needs a minimum of laboratory resources. It has been used for the last three years in our undergraduate medical physiology courses and has replaced the classic utilization of slides and drawings.
Corado
J
J
Fisiología y Biofísica, Facultad de Ciencias de la Salud, Universidad de Carabobo, El Trigal 2002, Valencia, Venezuela.
Eblen-Zajjur
A
A
D000818:Animals; D015550:Cell Degranulation; D004504:Education, Medical, Undergraduate / Q000379:methods*; D007753:Laboratories; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D010827:Physiology / Q000193:education*; D051381:Rats; D017207:Rats, Sprague-Dawley