Cloning, structural analysis and expression of the gene encoding aspartate aminotransferase from the thermophilic cyanobacterium Phormidium lapideum.
The aspartate aminotransferase gene from the thermophilic cyanobacterium Phormidium lapideum was cloned and expressed in Escherichia coli. The ORF of 1167 nucleotides encodes a protein of 388 amino acids having a molecular weight of 42,099. A molecular model of PIAspAT shows structural features similar to those of the Thermus thermophilus AspAT.
Kim
Hyeung
H
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 69028504, Japan.
Nakaoka
Masaki
M
Yagi
Mayumi
M
Ashida
Hiroyuki
H
Hamada
Kensaku
K
Shibata
Hitoshi
H
Sawa
Yoshihiro
Y