Identification of CRF10_CD viruses among bar and hotel workers in Moshi, Northern Tanzania.
We recently identified an HIV-1 subtype C and D circulating recombinant form (CRF10_CD) in infants in Dar es Salaam, Tanzania. So far, this is the only reported HIV-1 CRF in East Africa. However, evidence for its spread in the adult population is scarce. Here we describe the presence of CRF10_CD in two asymptomatic bar and hotel workers in Moshi, Northern Tanzania. Subgenomic sequences from gag (3'p24-5'p7), env (C2-C5), and the 5' LTR were used for phylogenetic analysis and identification of recombination. Genetic divergence between the CRF10_CD sequences from Moshi suggested that they were contracted from independent sources. A third bar worker was infected with an apparent CRF10_CD/subtype A recombinant virus. Our data indicate that CRF10_CD genomes can be transmitted both vertically and heterosexually.
Kiwelu
Ireen E
IE
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA.
Koulinska
Irene N
IN
Nkya
Watoky M M M
WM
Shao
John
J
Kapiga
Saidi
S
Essex
Max
M
D014644:Genetic Variation; D016679:Genome, Viral; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D010802:Phylogeny; D011995:Recombination, Genetic; D013636:Tanzania