Enzymatic synthesis of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) with CoA recycling using polyhydroxyalkanoate synthase and acyl-CoA synthetase.
We succeeded in developing a novel method for in vitro poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3 HB-co-4 HB)] synthesis with CoA recycling using polyhydroxyalkanoate synthase and an acyl-CoA synthetase. Using this method, the monomer compositions in P(3 HB-co-4 HB)s could be controlled strictly by the ratios of the monomers in the reaction mixtures.
Satoh
Yasuharu
Y
Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan. syasu@eng.hokudai.ac.jp
Murakami
Fumikazu
F
Tajima
Kenji
K
Munekata
Masanobu
M
D000217:Acyltransferases / Q000737:chemistry* / Q000235:genetics; D003065:Coenzyme A / Q000737:chemistry*; D003066:Coenzyme A Ligases / Q000737:chemistry* / Q000235:genetics; D020118:Cupriavidus necator / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics; D015202:Protein Engineering / Q000379:methods; D044204:Pseudomonas oleovorans / Q000201:enzymology* / Q000235:genetics
D029968:Escherichia coli Proteins; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase; D003066:Coenzyme A Ligases; C085934:acyl-coenzyme A synthetase, E coli; D003065:Coenzyme A