Development of highly potent and selective dynorphin A analogues as new medicines.
Dynorphin A (Dyn A), a 17 amino acid peptide H-Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln-OH, is a potent opioid peptide which interacts preferentially with kappa-opioid receptors. Research in the development of selective and potent opioid peptide ligands for the kappa-receptor is important in mediating analgesia. Several cyclic disulphide bridge-containing peptide analogues of Dyn A, which were conformationally constrained in the putative message or address segment of the opioid ligand, were designed, synthesized and assayed. To further investigate the conformational and topographical requirements for the residues in positions 5 and 11 of these analogues, a systematic series of Dyn A(1-11)-NH2 cyclic analogues incorporating the sulphydryl-containing amino acids L- and D-Cys and L- and D-Pen in positions 5 and 11 were synthesized and assayed. Cyclic lactam peptide analogues were also synthesized and assayed. Several of these cyclic analogues, retained the same affinity and selectivity (vs. the mu- and delta-receptors) as the parent Dyn A(1-11)-NH2 peptide in the guinea-pig brain (GPB), but exhibited a much lower activity in the guinea-pig ileum (GPI), thus leading to centrally vs. peripherally selective peptides. Studies of the structure-activity relationship of Dyn A peptide provide new insights into the importance of each amino acid residue (and their configurations) in Dyn A analogues for high potency and good selectivity at kappa-opioid receptors. We report herein the progress towards the development of Dyn A peptide ligands, which can act as agonists or antagonists at cell surface receptors that modulate cell function and animal behaviour using various approaches to rational peptide ligand-based drug design.
Lung
F-D T
FD
Department of Chemistry, Tunghai University, Taichung, Taiwan. fdlung@thu.edu.tw
Chen
C-H
CH
Liu
J-H
JH
D000818:Animals; D001681:Biological Assay; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002211:Capsaicin / Q000494:pharmacology; D015195:Drug Design; D004399:Dynorphins / Q000037:antagonists & inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D008024:Ligands; D008253:Macaca mulatta; D051379:Mice; D009130:Muscle, Smooth / Q000187:drug effects / Q000201:enzymology; D018847:Opioid Peptides / Q000819:agonists / Q000037:antagonists & inhibitors / Q000138:chemical synthesis; D010146:Pain / Q000188:drug therapy; D010455:Peptides / Q000037:antagonists & inhibitors / Q000737:chemistry; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors / Q000187:drug effects; D013045:Species Specificity
D008024:Ligands; D018847:Opioid Peptides; D010455:Peptides; D011956:Receptors, Cell Surface; D004399:Dynorphins; D002211:Capsaicin