Liquid chromatography/tandem mass spectrometric confirmatory method for determining aflatoxin M1 in cow milk: comparison between electrospray and atmospheric pressure photoionization sources.
A liquid chromatography/electrospray (ESI)-tandem mass spectrometric method for the measurement of aflatoxin M1 (AFM1) in milk is described. Milk sample after protein precipitation with acetone was cleaned-up with a Carbograph-4 cartridge. Performances of the ESI source were compared with those of the atmospheric pressure photoionization source (APPI). Although a method quantification limit (MQL) of 6 ng/kg could be achieved operating with APPI source with respect to an MQL of 12 ng/kg with ESI, all the other performances being similar, then ESI was preferred as being more robust and widespread at present.
Cavaliere
Chiara
C
Department of Chemistry, La Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Foglia
Patrizia
P
Pastorini
Elisabetta
E
Samperi
Roberto
R
Laganà
Aldo
A
D016604:Aflatoxin B1 / Q000032:analysis; D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D001274:Atmospheric Pressure; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D013058:Mass Spectrometry / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D016607:Aflatoxin M1; D016604:Aflatoxin B1