title: Liquid chromatography-mass spectrometry and related techniques for purity assessment in early drug discovery.
abstract: The combination of HPLC and MS has become the most valuable analytical tool to determine the identity and purity of a drug substance in the drug discovery arena over the past decade. This article describes different LC/MS configurations and their broad applicability to meet the fundamental analytical requirements involved in discovering new drugs. In addition, the value of chemiluminescence nitrogen detection for absolute purity determination and the convenience of CE as an orthogonal separation technique to HPLC are also discussed. In summary, LC/MS-based methodologies that implicate automation, various levels of throughput and open access systems have proved to be an integral part of the drug discovery process. As a result, the paradigm of high-quality/-quantity information is fulfilled in a timely fashion.
authors.affiliation: European Analytical Technologies DCR&T Alcobendas, Lilly, Madrid, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Molina-Martin
authors.forename: Aranzazu
authors.initials: A
authors.lastname: Marin
authors.forename: Alfonso
authors.initials: A
authors.lastname: Rivera-Sagredo
authors.forename: Alfonso
authors.initials: A
authors.lastname: Espada
mesh_terms: D002110:Caffeine / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D049673:History, 20th Century; D049674:History, 21st Century; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000266:history / Q000302:isolation & purification*
chemical_list: D004364:Pharmaceutical Preparations; D002110:Caffeine