Typing of food-borne Listeria monocytogenes by polymerase chain reaction-restriction enzyme analysis and amplified fragment length polymorphism.
The applicability of polymerase chain reaction-restriction enzyme analysis (PCR-REA) and amplified fragment length polymorphism (AFLP) for typing of food borne Listeria monocytogenes strains was tested. A panel of 43 L. monocytogenes strains isolated from food, mostly serovars 1/2b or 1/2a, were analysed by the optimized PCR-REA oriented to inlA and inlB genes and by AFLP. By PCR-REA, five types of profiles were obtained. By AFLP, the strains were separated into 11 types and 18 subtypes forming two major clusters. PCR REA was a relatively straightforward method for typing food-borne L. monocytogenes with a moderate discrimination power. AFLP was a more complex but a highly discriminative and reproducible method.
Mikasová
Eva
E
Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B-2, SK, Bratislava, Slovakia.
Oravcová
Katarína
K
Kaclíková
Eva
E
Kuchta
Tomás
T
Drahovská
Hana
H
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008565:Membrane Proteins / Q000235:genetics; D017720:Molecular Epidemiology; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; C095768:inlB protein, Listeria monocytogenes; C069761:internalin protein, Bacteria; D004262:DNA Restriction Enzymes