title: New, simple and validated UV-spectrophotometric methods for the estimation of gatifloxacin in bulk and formulations.
abstract: New, simple and cost effective UV-spectrophotometric methods were developed for the estimation of gatifloxacin in bulk and pharmaceutical formulations. Gatifloxacin was estimated at 286 nm in 100 mM phosphate buffer (pH 7.4) and 292 nm in 100 mM hydrochloric acid (pH 1.2). Linearity range was found to be 1-18 mug ml(-1) (regression equation: absorbance=0.0684 x Concentration in microg ml(-1) + 0.0050; r2 = 0.9998) in the phosphate buffer (pH 7.4) and 1-14 microg ml(-1) (regression equation: absorbance = 0.0864 x Concentration in microg ml(-1) + 0.0027; r2 = 0.9999) in hydrochloric acid medium (pH 1.2). The apparent molar absorptivity was found to be 2.62 x 10(4) l mol(-1) cm(-1) in the phosphate buffer and 3.25 x 10(4) l mol(-1) cm(-1) in hydrochloric acid media. In both the proposed methods sandell's sensitivity was found to be about 0.01 microg cm(-2)/0.001A. These methods were tested and validated for various parameters according to ICH guidelines and USP. The quantitation limits were found to be 0.312 and 0.3 microg ml(-1) in the phosphate buffer and hydrochloric acid medium, respectively. The proposed methods were successfully applied for the determination of gatifloxacin in pharmaceutical formulations (tablets, injection and ophthalmic solution). The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation <2%), while being simple, cheap and less time consuming and can be suitably applied for the estimation of gatifloxacin in different dosage forms and dissolution studies.
authors.affiliation: Pharmacy Group, Faculty Division III, Birla Institute of Technology and Science, Vidya Vihar, Pilani 333031, Rajasthan, India. venugopalk@bits-pilani.ac.in
authors.forename: K
authors.initials: K
authors.lastname: Venugopal
authors.forename: Ranendra N
authors.initials: RN
authors.lastname: Saha
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002138:Calibration; D024841:Fluoroquinolones / Q000032:analysis*; D000077734:Gatifloxacin; D006863:Hydrogen-Ion Concentration; D009883:Ophthalmic Solutions / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet / Q000379:methods* / Q000592:standards; D013607:Tablets / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D009883:Ophthalmic Solutions; D004364:Pharmaceutical Preparations; D013607:Tablets; D000077734:Gatifloxacin