title: An Alu-based, MGB Eclipse real-time PCR method for quantitation of human DNA in forensic samples.
abstract: The forensic community needs quick, reliable methods to quantitate human DNA in crime scene samples to replace the laborious and imprecise slot blot method. A real-time PCR based method has the possibility of allowing development of a faster and more quantitative assay. Alu sequences are primate-specific and are found in many copies in the human genome, making these sequences an excellent target or marker for human DNA. This paper describes the development of a real-time Alu sequence-based assay using MGB Eclipse primers and probes. The advantages of this assay are simplicity, speed, less hands-on-time and automated quantitation, as well as a large dynamic range (128 ng/microL to 0.5 pg/microL).
authors.affiliation: Vermont Forensic Laboratory, Department of Public Safety, 103 S. Main St., Waterbury, VT 05671, USA.
authors.forename: Janice A
authors.initials: JA
authors.lastname: Nicklas
authors.forename: Eric
authors.initials: E
authors.lastname: Buel
mesh_terms: D020087:Alu Elements; D000818:Animals; D052160:Benzothiazoles; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D015342:DNA Probes; D003959:Diamines; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences
chemical_list: D052160:Benzothiazoles; D017931:DNA Primers; D015342:DNA Probes; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D004247:DNA