title: [Prediction of lymph node metastasis with binary logistic regression in gastric carcinoma].
abstract: OBJECTIVE
  To investigate more specific markers to predict the lymph node metastasis in gastric carcinoma.
  
  
  METHODS
  The expression of heparanase mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in 43 cases with gastric cancer. The expressions of CD44V6, MMP-7, nm23 and syndecan-1 protein were examined by streptavidin-peroxidase (SP) two-step method. Clinicopathological features influencing lymphatic metastasis such as age,sex,tumor size,tumor location, Borrmann classification, histological type, differentiation and serosal infiltration were also analyzed.
  
  
  RESULTS
  Twenty-seven cases (62.8%) in 43 gastric cancer patients had lymphatic metastasis. The incidence of metastatic lymph nodes was(36.3 +/- 30.8)%. The median incidence was 19%. Univariate analysis showed that tumor size, serosal infiltration, expressions of heparanase mRNA, CD44V6, nm23 and syndecan-1 protein were risk factors for lymph node metastasis in gastric carcinoma. Multivariate analysis showed expressions of nm23 and syndecan-1 protein, serosal infiltration were independent factors for lymph node metastasis.
  
  
  CONCLUSIONS
  Gastric cancer with serosal infiltration, positive expressions of nm23 and syndecan-1 has greater possibility of lymph node metastasis.
authors.affiliation: Department of Gastrointestinopancreatic Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Jun-qiang
authors.initials: JQ
authors.lastname: Chen
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Zhan
authors.forename: Yu-long
authors.initials: YL
authors.lastname: He
authors.forename: Shi-rong
authors.initials: SR
authors.lastname: Cai
authors.forename: Jun-sheng
authors.initials: JS
authors.lastname: Peng
authors.forename: Zheng-xuan
authors.initials: ZX
authors.lastname: Chen
mesh_terms: D014408:Biomarkers, Tumor; D006801:Humans; D016015:Logistic Models; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis / Q000473:pathology*; D054778:NM23 Nucleoside Diphosphate Kinases / Q000378:metabolism; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D053668:Syndecan-1 / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D054778:NM23 Nucleoside Diphosphate Kinases; D012333:RNA, Messenger; D053668:Syndecan-1