title: Biochemical properties of C78SC96S rhFGF-2: a double point-mutated rhFGF-2 increases obviously its activity.
abstract: Fibroblast growth factor-2 (FGF-2) is a multifunctional polypeptide that affects many cellular functions and phenomena. The wild-type recombinant human fibroblast growth factor rhFGF-2(W) and the mutant C78SC96S rhFGF-2(M) were expressed in Escherichia coli and their products were purified. The results by the means of fluorescence spectroscopy and CD spectrums, suggested that due to its decreased hydrophobicity rhFGF-2 is not deposited as an inclusion body. The mitogenic activity of the expressed rhFGF-2(M) on 3T3 fibroblasts was shown to be 10-fold more than the expressed rhFGF-2(W) of which the biological activity was a little less than that of the standard rhbFGF(W), indicating that the increased biological activity was due to the change of its secondary structure, dimerization and affinity binding to FGF receptor (FGFR).
authors.affiliation: Bio-Engineering Institute of Jinan University, Guangzhou 510632, PR China. wangju1688@163.com
authors.forename: Ju
authors.initials: J
authors.lastname: Wang
authors.forename: An
authors.initials: A
authors.lastname: Hong
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Ren
authors.forename: Fen-Yong
authors.initials: FY
authors.lastname: Sun
authors.forename: Ying-Jiao
authors.initials: YJ
authors.lastname: Shi
authors.forename: Kan
authors.initials: K
authors.lastname: Liu
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Xie
authors.forename: Yun
authors.initials: Y
authors.lastname: Dai
authors.forename: Zhi-Ying
authors.initials: ZY
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
mesh_terms: D016475:3T3 Cells; D019943:Amino Acid Substitution; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D017354:Point Mutation; D017433:Protein Structure, Secondary / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2