Overexpression of Aspergillus aculeatus cellobiohydrolase I in Aspergillus oryzae.
To express the cbhI gene, encoding Aspergillus aculeatus cellobiohydrolase I (CBHI), in Aspergillus oryzae, a plasmid was constructed. The strain that displayed the strongest CBHI activity among the transformants produced about 941 mg/l in liquid culture. It was confirmed by a PCR method that the plasmid was integrated at the niaD locus.
Kanamasa
Shin
S
Graduate School ofAgriculture and Biological Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan.
Mochizuki
Miho
M
Takada
Goro
G
Kawaguchi
Takashi
T
Sumitani
Jun-Ichi
J
Araii
Motoo
M