Location of C. trachomatis Inc proteins during expression of their genes in HeLa cell culture.
Plasma constructions including genes encoding C. trachomatis inclusion membrane protein as composite proteins with reporter green fluorescent protein were obtained. After transfection of HeLa cell culture with the resultant plasmid constructions the location of inclusion membrane proteins in transfected cell was for the first time detected by confocal microscopy.
Kostryukova
E S
ES
Institute of Physicochemical Medicine, Ministry of Health of the Russian Federation, Moscow. eles@newmail.ru
Korobova
F V
FV
Lazarev
V N
VN
Shkarupeta
M M
MM
Titova
G A
GA
Akopian
T A
TA
Govorun
V M
VM
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002692:Chlamydia trachomatis / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins