A novel method for STR-based DNA profiling using microarrays.
We describe a novel method for rapidly identifying and distinguishing between different DNA sequences using short tandem repeat (STR) analysis and DNA microarrays. The method can be used to deduce identity, length, and number of STRs of the target molecule. We refer to this technique as the "variable-length probe array" method for STR profiling (VLPA). The method involves hybridization of the unknown STR target sequence to a DNA microarray displaying complementary probes that vary in length to cover the range of possible STRs. A post-hybridization enzymatic digestion of the DNA hybrids is then used to selectively remove labeled single-stranded regions of DNA from the microarray surface. The number of repeats in the unknown target is then deduced based on the pattern of target DNA that remains hybridized to the array. This DNA profiling technique is useful for performing forensic analysis to uniquely identify individual humans or other species.
Kemp
Jennifer T
JT
Stanford Genome Technology Center, 855 California Avenue, Palo Alto, CA 94304, USA.
Davis
Ronald W
RW
White
Robert L
RL
Wang
Shan X
SX
Webb
Chris D
CD
D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes; D017422:Sequence Analysis, DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases; D020080:Tandem Repeat Sequences
D015345:Oligonucleotide Probes; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases