title: Systematic transient assays of promoter activities for leaf-specific genes identified by gene-expression profiling with cDNA microarrays in Arabidopsis thaliana.
abstract: We identified 85 genes highly expressed in leaves using an Arabidopsis cDNA microarray. A vector, pRAB5, was designed to allow cloning and assaying of the promoters. Fifty-one promoters from the selected genes were cloned and then assayed using a microprojectile bombardment and dual luciferase reporter assay system. This system allowed efficient systematic assays of promoter activity.
authors.affiliation: Technology Department Research Association for Biotechnology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan. s-yamaka@bs.naist.jp
authors.forename: Seiyei
authors.initials: S
authors.lastname: Yamakawa
authors.forename: Kohei
authors.initials: K
authors.lastname: Ando
authors.forename: Aya
authors.initials: A
authors.lastname: Chisaka
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yoshida
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Shinmyo
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kohchi