title: Biodegradation of long-chain n-paraffins from waste oil of car engine by Acinetobacter sp.
abstract: Microorganisms that degrade long-chain n-paraffins from used car engine oil were isolated from soil. For the screening, a fraction of n-paraffin prepared from car engine oil was applied as the sole carbon source. The strain was identified as Acinetobacter sp. The ability of the strain to assimilate long-chain n-paraffins was assessed and characterized. The strain mineralized long-chain n-paraffins (0.1% w/v) in the minimal medium after cultivation for 96 h and also reduced the weight of the waste oil added (1% w/v) by 20% after 72 h without an extracellular biosurfactant. When n-hexadecane was fed as substrate, 1-hexadecanol and 1-hexadecanoic acid were detected as the intermediates by gas chromatography/mass spectrometry. This indicates that the long-chain n-paraffins were metabolized via the terminal oxidation pathway of n-alkane.
authors.affiliation: Department of Bioscience & Technology, Faculty of Science & Engineering, Ritsumeikan University, Nojihigashi, Kusatsu-shi, Shiga-ken 525-8577, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Koma
authors.forename: F
authors.initials: F
authors.lastname: Hasumi
authors.forename: E
authors.initials: E
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Ohta
authors.forename: S Y
authors.initials: SY
authors.lastname: Chung
authors.forename: M
authors.initials: M
authors.lastname: Kubo