Change in diazepam sensitivity of GABAA currents after LTP induction in neurons of deep cerebellar nuclei.
In deep cerebellar nuclei (DCN) neurons, inhibitory postsynaptic currents (IPSCs) undergo long-term depression (LTD) following a 10-Hz stimulation, and long-term potentiation (LTP) after a 100 Hz stimulation of the inputs. Whole-cell recordings were made from DCN neurons and changes in IPSC sensitivity to diazepam after LTD and LTP investigated. Diazepam enhanced the evoked IPSC amplitude by 45% in controls and after LTD induction. However, after LTP induction, diazepam increased the IPSC by only 16%. Diazepam increased THIP response by 34% in controls, but by only 4% after LTP. These results suggest that during LTP the diazepam sensitive GABAA receptor sub-units undergo changes.
Ouardouz
M
M
Neuroscience Research Laboratory, Department of Pharmacology and Therapeutics, Faculty of Medicine, The University of British Columbia, 2176 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3. mouardouz@ohri.ca
Sastry
B R
BR
D000818:Animals; D000831:Animals, Newborn; D002529:Cerebellar Nuclei / Q000166:cytology*; D003975:Diazepam / Q000494:pharmacology*; D004558:Electric Stimulation / Q000379:methods; D018757:GABA Modulators / Q000494:pharmacology*; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D036881:Long-Term Synaptic Depression / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D009474:Neurons / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D018408:Patch-Clamp Techniques / Q000379:methods; D051381:Rats; D011963:Receptors, GABA-A / Q000502:physiology*
D018757:GABA Modulators; D011963:Receptors, GABA-A; D003975:Diazepam