Effect of osmotic stabilizers on protoplast generation of Chlorella ellipsoidea yellow/white color mutants.
The effect of osmotic stabilizers on protoplast formation of yellow/white color mutants of Chlorella ellipsoidea was studied. The UF-1 strain, one of the mutants, had the same growth rate as the parent C. ellipsoidea at 4.02 microE/m2/s and no reversion to normal cells at 0-26.8 microE/m2/s. Therefore, protoplast formation from UF-1 was carried out, and it was found that the yield of protoplasts was 93% in the medium containing 1.0 M NaCl as an osmotic stabilizer.
Urano
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Laboratory of Marine Biochemistry, Tokyo University of Fisheries, Konan, Minato, Tokyo 108-8477, Japan.
Fujii
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