Intracellular FITC-derivatization with PEG.
In order to investigate the amino acids (AAs) in plant cells, we explore an avenue for intracellular derivatization with FITC. In this method, FITC was used to mark AAs in living protoplasts derived from embryogenic calli of common wheat (Triticum aestivum L. c.v. Jinan 177) mediated by PEG. After FITC-derivatization, the AAs in the lysate were determined by CE. The result reveals that this PEG method can be used to transfer FITC into plant cells efficiently, which provides a good method for AA analysis in plant cells.
Chen
Fanguo
F
School of Life Science, Shandong University, Jinan, P.R. China.
Zhi
Daying
D
Xia
Guangmin
G
D000596:Amino Acids / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D059828:Plant Cells; D010944:Plants / Q000737:chemistry; D011092:Polyethylene Glycols; D011523:Protoplasts / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry
D000596:Amino Acids; D005456:Fluorescent Dyes; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate