title: Development of a solid phase microextraction-gas chromatography method to determine N-hydroxymethyl-N-methylformamide and N-methylformamide in urine.
abstract: A headspace solid phase microextraction (SPME) method has been developed to determine metabolites of dimethylformamide, N-hydroxymethyl-N-methylformamide, and N-methylformamide (NMF) as NMF in urine by gas chromatography with nitrogen-phosphorus detector (GC-NPD). An SPME holder with a 65-microm PDMS/DVB fiber coating was used. Optimal desorption conditions were 250 degrees C for 1 min, adsorption at 80 degrees C for 15 min, and 3.00 mL of sample in the headspace vial. The method presented good resolution, repeatability, recovery, detection limit, ruggedness and response linearity.
authors.affiliation: Chemistry Department, ICEx, Federal University of Minas Gerais, Av. Antonio Carlos 6627, C.P. 702, 31270901 Belo Horizonte, M.G., Brazil.
authors.forename: Vagner Fernandes
authors.initials: VF
authors.lastname: Knupp
authors.forename: Edna Maria Alvarez
authors.initials: EM
authors.lastname: Leite
authors.forename: Zenilda
authors.initials: Z
authors.lastname: de Lourdes Cardeal
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D004126:Dimethylformamide / Q000031:analogs & derivatives* / Q000032:analysis; D005559:Formamides / Q000032:analysis*; D006801:Humans; D015203:Reproducibility of Results; D014556:Urine / Q000737:chemistry*
chemical_list: D005559:Formamides; C042331:N-hydroxymethyl-N-methylformamide; D004126:Dimethylformamide; C002950:methylformamide