A real-time PCR assay for the specific identification of serotype O:9 of Yersinia enterocolitica.
A real-time PCR assay was developed based on a 181-bp fragment of the recently cloned per gene, including an internal amplification control (124 bp), for the detection of Yersinia enterocolitica O:9 (Ye O:9). The validation included 48 Ye O:9, 33 Y. enterocolitica non-O:9 and 35 other closely-related bacterial strains, containing per gene homologies. The assay was specific for the Ye O:9 tested, the detection limit was 1-10 genome copies of purified DNA and amplification efficiency was between 90.5-103%, indicating a linear regression throughout the detection window.
Jacobsen
N R
NR
Danish Institute for Food and Veterinary Research (DFVF), Copenhagen, Denmark.
Bogdanovich
T
T
Skurnik
M
M
Lübeck
P S
PS
Ahrens
P
P
Hoorfar
J
J
D000818:Animals; D002238:Carbohydrate Epimerases / Q000235:genetics*; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping; D013045:Species Specificity; D000637:Transaminases / Q000235:genetics*; D015008:Yersinia enterocolitica / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial; D000637:Transaminases; D002238:Carbohydrate Epimerases; C061272:perosamine synthetase