Recent developments in analytical methodology for 8-hydroxy-2'-deoxyguanosine and related compounds.
When biomolecules such as proteins, lipids, and DNA are subjected to oxidative attack by free radicals or other reactive species, a number of measurable biomarkers may be produced. The study of oxidative DNA damage is valuable in research concerning cancer and aging. The current review includes methodology involving various separation science techniques for the analysis of DNA oxidation biomarkers, mainly 8-hydroxy-2'-deoxyguanosine. This review will present recent analytical developments with respect to sample preparation and instrumental considerations, noting key outcomes and biological relevance where appropriate.
Peoples
Michael C
MC
Department of Pharmaceutics, Virginia Commonwealth University Medical Center, P.O. Box 980533, Richmond, VA 23298-0533, USA.
Karnes
H Thomas
HT
D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods; D018384:Oxidative Stress / Q000502:physiology; D010761:Phosphorus Radioisotopes
D015415:Biomarkers; D005456:Fluorescent Dyes; D010761:Phosphorus Radioisotopes; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D016650:Fluorescein-5-isothiocyanate