Close correlation of streptococcal DNase B (sdaB) alleles with emm genotypes in Streptococcus pyogenes.
DNase B is a major nuclease and a possible virulence factor in Streptococcus pyogenes. The allelic diversity of streptococcal DNase B (sdaB) gene was investigated in 83 strains with 14 emm genotypes. Of the 15 alleles identified, 11 alleles carried only synonymous nucleotide substitutions. On the other hand, 4 alleles had a non-synonymous substitution other than synonymous substitutions, resulting in the substitution of a single amino acid. The distribution of each allele was generally emm genotype-specific. Only sdaB7 was found in both emm2 and emm4. The promoter region was highly conserved and DNase B protein was similarly expressed in all alleles.
Matsumoto
Masakado
M
Department of Microbiology, Aichi Prefectural Institute of Public Health, Nagoya, Japan. masakado_matsumoto@pref.aichi.lg.jp
Sakae
Kenji
K
Hashikawa
Shinnosuke
S
Torii
Keizo
K
Hasegawa
Tadao
T
Horii
Toshinobu
T
Endo
Miyoko
M
Okuno
Rumi
R
Murayama
Shoko
S
Hirasawa
Kyoko
K
Suzuki
Rieko
R
Isobe
Junko
J
Tanaka
Daisuke
D
Katsukawa
Chihiro
C
Tamaru
Aki
A
Tomita
Masaaki
M
Ogata
Kikuyo
K
Ikebe
Tadayoshi
T
Watanabe
Haruo
H
Ohta
Michio
M
D000483:Alleles; D019943:Amino Acid Substitution; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D003851:Deoxyribonucleases / Q000032:analysis / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D005838:Genotype; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013223:Statistics as Topic; D013297:Streptococcus pyogenes / Q000201:enzymology / Q000235:genetics*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; C014689:streptococcal M protein; D003851:Deoxyribonucleases; C020069:deoxyribonuclease B