title: Electromagnetic fields inhibit endothelin-1 production stimulated by thrombin in endothelial cells.
abstract: Electromagnetic field (EMF) radiation has been found to induce arteriolar dilatation, but the mechanism of action remains largely unknown. This study investigated the effect of EMF radiation on the production of endothelin-1 (ET-1), a potent vasoconstrictor, by cultured endothelial cells. EMF radiation reduced ET-1 basal levels in human umbilical vein and microvascular endothelial cells, but failed to reduce ET-1 basal levels in bovine and human aortic endothelial cells. EMF radiation significantly inhibited thrombin-stimulated ET-1 production in all four endothelial cell types in a dose-dependent manner. EMF radiation significantly inhibited thrombin-induced endothelin-1 mRNA expression in all four cell types. The inhibitory effect of EMF radiation on ET-1 production was abolished by the nitric oxide synthase inhibitor NG-monomethyl-L-arginine (10(-3) mol/1). These results demonstrate that EMF radiation modulates ET-1 production in cultured vascular endothelial cells and the inhibitory effect of EMF radiation is, at least partly, mediated through a nitric oxide-related pathway.
authors.affiliation: Department of Geriatric Medicine, Kanazawa Medical University, Ishikawa, Japan. shigeto@kanazawa-med.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Morimoto
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Kanda
authors.forename: K
authors.initials: K
authors.lastname: Okaishi
authors.forename: M
authors.initials: M
authors.lastname: Okuro
authors.forename: H
authors.initials: H
authors.lastname: Murai
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimura
authors.forename: K
authors.initials: K
authors.lastname: Nomura
authors.forename: H
authors.initials: H
authors.lastname: Tsuchiya
authors.forename: I
authors.initials: I
authors.lastname: Ohashi
authors.forename: M
authors.initials: M
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D001011:Aorta / Q000033:anatomy & histology; D002417:Cattle; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D019332:Endothelin-1 / Q000235:genetics / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology; D004791:Enzyme Inhibitors / Q000378:metabolism; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism*; D014471:Umbilical Veins / Q000033:anatomy & histology; D019323:omega-N-Methylarginine / Q000378:metabolism
chemical_list: D019332:Endothelin-1; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D013917:Thrombin