Contribution of conserved glycine residues to ATP action at human P2X1 receptors: mutagenesis indicates that the glycine at position 250 is important for channel function.
Glycine residues can introduce flexibility in proteins, give rise to turns and breaks in secondary structure and are key components of some nucleotide binding motifs. In the P2X receptor extracellular ATP binding domain, 11 glycine residues are completely conserved and an additional five are conserved in at least five of the seven family members. We have mutated individual conserved glycine residues and determined their effect on the ATP sensitivity and time-course of P2X1 receptors expressed in Xenopus oocytes. In the majority of cases, replacement by alanine had no or a less than 3-fold effect on ATP sensitivity and time-course of responses. G71A resulted in a 6-fold decrease in ATP potency and ATP (10 mM) failed to evoke functional responses from G96A, G250A and G301A mutant receptors. However, proline or cysteine could substitute for glycine at positions 96 and 301, giving receptors that were essentially normal. At glycine 250 substitution by serine gave functional responses to ATP with no effect on ATP sensitivity but a reduction in peak amplitude; in contrast, functional responses were not recorded when glycine 250 was replaced by the amino acids alanine, cysteine, aspartate, phenylalanine, isoleucine, lysine, proline or asparagine. These results suggest that glycine 250 plays an important role in determining the function of P2X receptors.
Digby
Helen R
HR
Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, UK.
Roberts
Jonathan A
JA
Sutcliffe
Michael J
MJ
Evans
Richard J
RJ
D000255:Adenosine Triphosphate / Q000502:physiology*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D015153:Blotting, Western; D004594:Electrophysiology; D005110:Extracellular Space / Q000378:metabolism; D005998:Glycine / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D007473:Ion Channels / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009865:Oocytes / Q000378:metabolism; D018408:Patch-Clamp Techniques; D018048:Receptors, Purinergic P2 / Q000235:genetics / Q000502:physiology*; D058469:Receptors, Purinergic P2X; D012694:Serine / Q000235:genetics / Q000502:physiology; D014981:Xenopus
D007473:Ion Channels; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D012694:Serine; D000255:Adenosine Triphosphate; D005998:Glycine