title: Effect of heavy metals on the growth of a methanogen in pure culture and coculture with a sulfate-reducing bacterium.
abstract: The sensitivity of a methanogen and sulfate-reducing bacterium isolated from a sea-based landfill site to Cd2+ and Cu2+ was studied. Methanogens and sulfate-reducing bacteria in leachates of the waste disposal site were enumerated using the MPN method. Methanobacterium thermoautotrophicum KHT-2, isolated from the leachate, could not grow at 0.5 mM Cd2+ or 1.0 mM Cu2+. Desulfotomaculum sp. RHT-3, isolated from the same leachate, was able to insolubilize 3.0 mM Cd2+ or 2.0 mM Cu2+ by production of hydrogen sulfide. When strains KHT-2 and RHT-3 were cultured together in the presence of the heavy metals, strain KHT-2 could grow at high heavy metal concentrations after insolubilization of the metals by strain RHT-3.
authors.affiliation: Department of Bioprocessing, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mori
authors.forename: M
authors.initials: M
authors.lastname: Hatsu
authors.forename: R
authors.initials: R
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Takamizawa