title: Cloning, structural analysis and expression of the gene encoding aspartate aminotransferase from the thermophilic cyanobacterium Phormidium lapideum.
abstract: The aspartate aminotransferase gene from the thermophilic cyanobacterium Phormidium lapideum was cloned and expressed in Escherichia coli. The ORF of 1167 nucleotides encodes a protein of 388 amino acids having a molecular weight of 42,099. A molecular model of PIAspAT shows structural features similar to those of the Thermus thermophilus AspAT.
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 69028504, Japan.
authors.forename: Hyeung
authors.initials: H
authors.lastname: Kim
authors.forename: Masaki
authors.initials: M
authors.lastname: Nakaoka
authors.forename: Mayumi
authors.initials: M
authors.lastname: Yagi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ashida
authors.forename: Kensaku
authors.initials: K
authors.lastname: Hamada
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shibata
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sawa