title: Insulinoma cell line (MIN6) adhesion and spreading mediated by Arg-Gly-Asp (RGD) sequence conjugated in thermo-reversible gel.
abstract: We have functionalized gels with a putative cell-binding (-Arg-Gly-Asp-) (RGD) domain in an effort to regulate mammalian cell behavior in cells entrapped with gel. Adhesion molecules composed of Gly-Arg-Gly-Asp-Ser (GRGDS) peptides and cell recognition ligands were inculcated into thermo-reversible hydrogel composed of N-isopropylacrylamide, with a small amount of succinyl poly(ethylene glycol) (PEG) acrylate (MW 2000) used as a biomimetic extracellular matrix (ECM). The GRGDS-containing p(NiPAAm-co-PEG) copolymer gel was studied in vitro for its ability to promote cell spreading and to increase the viability of cells by introducing PEG spacers. Hydrogel lacking the adhesion molecules proved to be a poor ECM for adhesion, permitting only a 20% spread of the seeded cells after 10 d. When PEG spacer arms, immobilized by a peptide linkage, had been integrated into the hydrogel, conjugation of RGD promoted cell spread by 300% in a 28-d trial. In addition, in a serum-free medium, only GRGDS peptides conjugated with the spacer arm were able to promote cell spread.
authors.affiliation: College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute, 605 Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Korea.
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
authors.forename: Kun
authors.initials: K
authors.lastname: Na
authors.forename: Seung Yong
authors.initials: SY
authors.lastname: Jung
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Kyu Hyung
authors.initials: KH
authors.lastname: Park
authors.forename: Kwang Yul
authors.initials: KY
authors.lastname: Cha
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Chung
mesh_terms: D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D007340:Insulinoma / Q000473:pathology / Q000503:physiopathology; D007515:Islets of Langerhans / Q000166:cytology* / Q000502:physiology*; D008422:Materials Testing; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D020100:Hydrogels; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid