Analysis of polymorphism of 18S rRNA gene in Wuchereria bancrofti microfilariae.
The polymorphism of the 18S rRNA gene in Wuchereria bancrofti microfilariae (mf) collected from three different zones in India was analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The RFLPs of the amplified products obtained after digestion with restriction enzymes Ssp I, Msp I and Hha I showed no difference in the banding patterns among the mf isolates from different endemic zones. Further the sequencing of PCR products did not show any difference in the nucleotide sequence either. The phylogenetic analysis of the sequences of W. bancrofti mf isolates from different endemic zones has shown branching with the earlier reported sequences of W. bancrofti and its close relative Brugia malayi.
Bhandari
Yashodhar
Y
Department of Biochemistry & J.B. Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.
Dabir
Pankaj
P
Nandhakumar
Krithika
K
Dayananda
Kannayakanahalli Maheshwarappa
KM
Shouche
Yogesh Shripad
YS
Reddy
Maryada Venkata Rami
MV
D000818:Animals; D017178:Brugia malayi / Q000235:genetics; D004247:DNA / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics*; D019003:Deoxyribonuclease HpaII / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D019353:Endemic Diseases; D005368:Filariasis / Q000469:parasitology; D017238:Genes, Helminth; D020459:Genes, rRNA; D006801:Humans; D007194:India; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014958:Wuchereria bancrofti / Q000235:genetics* / Q000302:isolation & purification
D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D004247:DNA; D019003:Deoxyribonuclease HpaII; C061568:endodeoxyribonuclease SspI; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases