[The pulmonary protective effects of leukocyte depletion with a modified filter located at the cardiopulmonary bypass (CPB) venous line in canine].
OBJECTIVE
To investigate the pulmonary protective effects of short-term leukocyte depletion with a modified filter located at the CPB venous line in canine.


METHODS
Sixteen healthy hybrid canines were randomly allocated to the LD-1 group, with an LD-1 filter installed in the CPB venous-line for a 5-minute leukocyte depletion at the beginning of the CPB, and to the control group. Blood samples were taken for routine test respectively at 9 time-points, namely pre-CPB, at 5, 10, 40, 70 min of the CPB, 5 min after aortic cross-clamp being off, at the discontinuation of the CPB, 5 min after protamine being administered, and 2 h after the CPB. Oxygen index (OI), pulmonary pathology and levels of interleukin-6 (IL-6) and interleukin-8 (IL-8) in plasma were measured at pre-CPB, 5 min after protamine being administered and 2 h after the CPB respectively. The levels of IL-6 and IL-8 in the bronchial alveolar lavage fluid (BALF) were measured at 2 h after the CPB. RESULTS; In LD-1 group, white blood cell (WBC) counts were significantly less than those in control group at 5 min of the CPB (P < 0.05), the filtration rate of WBC was (65.72 +/- 9.36)%. The WBC counts showed no significant difference in either group from 40 min of the CPB to 2 h after the CPB. OI was higher in LD-1 group than that in control group (P < 0.05) at 5 min after protamine being administered and 2 h after the CPB. There was significantly less WBC infiltration and alveolar edema of the lung in LD-1 group than that in control group at 5 min after protamine being administered and 2 h after the CPB. There were no significant differences of IL-6 and IL-8 in plasma between the two groups at 5 min after protamine being administered (P > 0.05), but the levels of IL-6 and IL-8 were much lower in the LD-1 group than those in the control group at 2 h after CPB. At 2 h after the CPB, IL-6 level in the BALF showed no significant difference in each group (P > 0.05); however, the level of IL-8 was lower in LD-1 group than that in control group (P < 0.05).


CONCLUSIONS
The LD-1 filter in the CPB venous-line in canine model used for short-term leukocyte depletion filtration could reduce WBC counts significantly, decrease the levels of IL-6 and IL-8 in plasma, attenuate inflammatory response in the lung after CPB, so it has protective effect on canine lung.
Li
Qi
Q
Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu 610041, China.
Chen
Hui-Jiao
HJ
Du
Lei
L
Zhou
Ling
L
Wang
Ying
Y
Liu
Bin
B
D000818:Animals; D002315:Cardiopulmonary Bypass / Q000379:methods*; D004285:Dogs; D005260:Female; D007958:Leukocyte Count; D047589:Leukocyte Reduction Procedures / Q000295:instrumentation*; D008297:Male; D011897:Random Allocation; D012128:Respiratory Distress Syndrome / Q000097:blood / Q000209:etiology / Q000517:prevention & control*