Automated prescan function for scanning fluorescently stained 2D-PAGE gels.
To automate the acquisition of images from fluorescently stained gels, the power of the excitation laser(s) must be optimized for each sample to prevent spot saturation (or to allow unimportant spots to saturate) yet still retaining sensitivity. In this work, we describe the implementation and effectiveness of a pre-scan function in a robotic solution for the automation of 2D gel scanning.
Bäck
Peter
P
Protein Technology, Wallenberg Laboratory II, Lund University, P.O. Box 7031, SE-220 07 Lund, Sweden.
Bengtsson
Sofia
S
James
Peter
P
D000465:Algorithms; D001331:Automation; D001798:Blood Proteins / Q000737:chemistry; D002232:Carbocyanines / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007834:Lasers; D008027:Light; D012680:Sensitivity and Specificity; D012984:Software
D001798:Blood Proteins; D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3; C085321:cyanine dye 5; C011203:cyanine dye 2