title: Promoter of Arabidopsis thaliana phosphate transporter gene drives root-specific expression of transgene in rice.
abstract: The PHT1 promoter::GUS fusion gene was constructed and introduced into Arabidopsis and rice by Agrobacterium-mediated transformation. Strong beta-glucuronidase (GUS) activity was detected in roots and showed phosphate starvation induction both in Arabidopsis and rice. In contrast, GUS activity in aerial tissues such as those of the leaf and stem was low. In situ GUS staining of root tissue indicated that PHT1 was expressed in root hairs and the outer layer of the main roots, but not in root tips. The PHT1 promoter has a desirable character for biotechnological transgene expression in monocot rice plants.
authors.affiliation: Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Koyama
authors.forename: Toshiro
authors.initials: T
authors.lastname: Ono
authors.forename: Masami
authors.initials: M
authors.lastname: Shimizu
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Jinbo
authors.forename: Rie
authors.initials: R
authors.lastname: Mizuno
authors.forename: Keiji
authors.initials: K
authors.lastname: Tomita
authors.forename: Norihiro
authors.initials: N
authors.lastname: Mitsukawa
authors.forename: Tetsu
authors.initials: T
authors.lastname: Kawazu
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kimura
authors.forename: Kunio
authors.initials: K
authors.lastname: Ohmiya
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D018014:Gene Transfer Techniques; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D028061:Phosphate Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C494975:PT2 protein, Arabidopsis; D028061:Phosphate Transport Proteins; D011994:Recombinant Proteins