title: A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater.
abstract: The biological process for phosphorus removal from wastewater is based on the use of bacteria capable of accumulating inorganic polyphosphate (polyp). We previously showed that a phoU mutation leads to polyp accumulation in Escherichia coli. The phoU mutant could be easily screened on agar plates containing 5-bromo-4-chloro-3-indolyl-phosphate (X-P(i)) after N-methyl-N'-vitro-N-nitrosoguanidine (NTG) mutagenesis. Here, we demonstrate that this method is also useful for screening polyp-accumulating mutants of bacterial strains isolated from soil and activated sludge samples.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Morohoshi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamashita
authors.forename: Junichi
authors.initials: J
authors.lastname: Kato
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Ikeda
authors.forename: Noboru
authors.initials: N
authors.lastname: Takiguchi
authors.forename: Hisao
authors.initials: H
authors.lastname: Ohtake
authors.forename: Akio
authors.initials: A
authors.lastname: Kuroda