title: Cytochrome c oxidase purified from a mercury-resistant strain of Acidithiobacillus ferrooxidans volatilizes mercury.
abstract: We suggested in our previous study that the plasma membrane cytochrome c oxidase of the mercury-resistant iron-oxidizing bacterial strain Acidithiobacillus ferrooxidans, SUG 202, is involved in Fe2+-dependent mercury volatilization. To study the involvement of A. ferrooxidans cytochrome c oxidase in mercury reduction, the cytochrome c oxidase was extracted from mercury-resistant and mercury-sensitive strains and purified. The Fe2+-dependent mercury volatilization activities of the oxidases from these strains were compared. The cytochrome c oxidase from strain SUG 2-2 volatilized 39% of the total Hg2+ (7 nmol) that had been added to a 10-ml reaction mixture (pH 3.8) in the presence of 10 micromol of Fe2+ after a 7-d incubation period at 30 degrees C. In contrast, the enzyme purified from the mercury-sensitive strain AP19-3 volatilized 3.5% of the total mercury under the same conditions. The boiled SUG 2-2 oxidase did not exhibit activity to volatilize mercury. Fe2+ reduced the oxidase from SUG 2-2 and Hg2+ oxidized the reduced enzyme. The purified SUG 2-2 oxidase is composed of three protein subunits with apparent molecular weights of 56,000 Da (alpha), 24,000 Da (beta), and 19,000 Da (gamma). The amount of mercury bound to the purified SUG 2-2 oxidase was 6.2 microg/mg protein and those bound to alpha-, beta- and gamma-subunits of the cytochrome c oxidase were 3.5, 2.6 and 0.7 microg/mg protein, respectively.
authors.affiliation: Division of Science and Technology for Energy Conversion, Graduate School of Natural Science and Technology, Okayama University, Tsushima Naka, Okayama 700-8530, Japan. sugio@cc.okayama-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Sugio
authors.forename: K
authors.initials: K
authors.lastname: Iwahori
authors.forename: F
authors.initials: F
authors.lastname: Takeuchi
authors.forename: A
authors.initials: A
authors.lastname: Negishi
authors.forename: T
authors.initials: T
authors.lastname: Maeda
authors.forename: K
authors.initials: K
authors.lastname: Kamimura