Production of two types of ice crystal-controlling proteins in Antarctic bacterium.
Pseudomonas fluorescens KUAF-68, which was isolated from Antarctica, had both ice-nucleating protein and antifreeze protein activities in the culture broth. We found that both proteins were separately produced based on the results of column chromatography, SDS-PAGE analysis and Southern hybridization. The activity of the ice-nucleating protein was stimulated by the addition of glycine (0.020 N%), whereas the activity of the antifreeze protein was stimulated by the addition of L-asparagine (0.025 N%). This is the first report on the production of two types of ice crystal-controlling proteins in one bacterial strain.
Kawahara
Hidehisa
H
Department of Biotechnology, Faculty of Engineering, Kansai University, 3-3-35 Yamate-cho, Suita-shi, Osaka 564-8680, Japan. kawahara@ipcku.kansai-u.ac.jp
Nakano
Yoko
Y
Omiya
Kazuhiro
K
Muryoi
Naomi
N
Nishikawa
Jiro
J
Obata
Hitoshi
H