Structure and mechanism of DNA polymerases.
DNA polymerases are molecular motors directing the synthesis of DNA from nucleotides. All polymerases have a common architectural framework consisting of three canonical subdomains termed the fingers, palm, and thumb subdomains. Kinetically, they cycle through various states corresponding to conformational transitions, which may or may not generate force. In this review, we present and discuss the kinetic, structural, and single-molecule works that have contributed to our understanding of DNA polymerase function.
Rothwell
Paul J
PJ
Institute of Structural Molecular Biology, University College London and Birkbeck College, Malet Street, London WC1E 7HX, United Kingdom.
Waksman
Gabriel
G
D000818:Animals; D002384:Catalysis; D004247:DNA / Q000737:chemistry / Q000502:physiology; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000502:physiology*; D006801:Humans; D009711:Nucleotides / Q000737:chemistry / Q000502:physiology; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
D009711:Nucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase