title: Lipase and antibacterial activities of a recombinant protein from the accessory glands of female Phlebotomus papatasi (Diptera: Psychodidae).
abstract: The reproductive accessory glands of the adult female have a functional versatility in insects, contributing to reproduction in various ways. The major protein secreted by the accessory glands of female Phlebotomus papatasi (Diptera, Psychodidae) has already been characterised and named PhpaLIP (for P. papatasi lipase) because, in terms of its amino-acid sequence, it is very similar to a wide range of vertebrate lipases. The gene coding for PhpaLIP has now been cloned into a pQE30 vector and expressed in Escherichia coli. When the recombinant PhpaLIP was tested in vitro, it was found to have not only lipase-like activity (when p-nitrophenyl caprylate was used as the substrate) but also specific antibacterial activity against some Gram-positive and Gram-negative bacteria. The possible physiological roles of PhpaLIP in P. papatasi are discussed, in the light of these results.
authors.affiliation: Dipartimento di Scienze Ambientali, Largo dell'Università, Università della Tuscia, 01100 Viterbo, Italy. ma_cri@inwind.it
authors.forename: M
authors.initials: M
authors.lastname: Belardinelli
authors.forename: A M
authors.initials: AM
authors.lastname: Fausto
authors.forename: L
authors.initials: L
authors.lastname: Guerra
authors.forename: F
authors.initials: F
authors.lastname: Buonocore
authors.forename: G
authors.initials: G
authors.lastname: Bongiorno
authors.forename: M
authors.initials: M
authors.lastname: Maroli
authors.forename: M
authors.initials: M
authors.lastname: Mazzini
mesh_terms: D000192:Actinomycetales / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism*; D001407:Bacillus / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D005836:Genitalia, Female / Q000378:metabolism*; D007303:Insect Vectors / Q000378:metabolism; D008826:Microbial Sensitivity Tests / Q000379:methods; D010691:Phlebotomus / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D002265:Carboxylic Ester Hydrolases