Micromechanical mass sensors for biomolecular detection in a physiological environment.
Micromechanical cantilever arrays are used to measure time-resolved adsorption of tiny masses based on protein-ligand interactions. Here, streptavidin-biotin interactions are investigated in a physiological environment. A measurement method is introduced using higher flexural modes of a silicon cantilever in order to enhance the sensitivity of mass detection. Modeling the cantilever vibration in liquid allows the measurement of absolute mass changes. We show time-resolved mass adsorption of final 7+/-0.7 ng biotinylated latex beads. The sensitivity obtained is about 2.5 pg/Hz measuring at a center frequency of 750 kHz.
Braun
Thomas
T
National Center of Competence for Research in Nanoscience, Institute of Physics, University of Basel, 4056 Basel, Switzerland.
Barwich
Viola
V
Ghatkesar
Murali Krishna
MK
Bredekamp
Adriaan H
AH
Gerber
Christoph
C
Hegner
Martin
M
Lang
Hans Peter
HP
D001696:Biomechanical Phenomena / Q000295:instrumentation* / Q000379:methods; D001704:Biopolymers / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008970:Molecular Weight; D025941:Protein Interaction Mapping / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014159:Transducers; D014732:Vibration
D001704:Biopolymers