A composite of hydroxyapatite with electrospun biodegradable nanofibers as a tissue engineering material.
Biodegradable and biocompatible poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a copolymer of microbial polyester, was fabricated as a nanofibrous film by electrospinning and composited with hydroxyapatite (HAp) by soaking in simulated body fluid. Compared with a PHBV cast (flat) film, the electrospun PHBV nanofibrous film was hydrophobic. However, after HAp deposition, both of the surfaces were extremely hydrophilic. The degradation rate of HAp/PHBV nanofibrous films in the presence of polyhydroxybutyrate depolymerase was very fast. Nanofiber formation increased the specific surface area and HAp enhanced the invasion of enzyme into the film by increasing surface hydrophilicity. The surface of the nanofibrous film showed enhanced cell adhesion over that of the flat film, although cell adhesion was not significantly affected by the combination with HAp.
Ito
Yoshihiro
Y
Kanagawa Academy of Science and Technology, KSP East 309, 3-2-1 Sakado, Takatsu-ku, Kawasaki 213-0012, Japan. y-ito@riken.jp
Hasuda
Hirokazu
H
Kamitakahara
Masanobu
M
Ohtsuki
Chikara
C
Tanihara
Masao
M
Kang
Inn-Kyu
IK
Kwon
Oh Hyeong
OH
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000032:analysis / Q000737:chemistry*; D019556:COS Cells; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D002522:Chlorocebus aethiops; D017886:Durapatite / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D008422:Materials Testing; D043942:Nanotubes / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011091:Polyesters / Q000032:analysis / Q000737:chemistry*; D012399:Rotation; D013499:Surface Properties; D013784:Textiles; D023822:Tissue Engineering / Q000379:methods*; D000094122:Polyhydroxybutyrates
D001672:Biocompatible Materials; D017886:Durapatite; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates