In planta transformation of kenaf plants (Hibiscus cannabinus var. aokawa No. 3) by Agrobacterium tumefaciens.
Kenaf was transformed by inoculation of Agrobacterium tumefaciens onto the meristems of young plants in pots. The transformation was demonstrated by three lines of evidence: a phenotypic inheritance from T(0) to T(1) plants, detection of the transgene in both T(0) and T(1) plants, and rescue of plasmids composed of T-DNA of the binary vector and flanking plant genomic DNA from T(1) plants.
Kojima
Mineo
M
Research Center for Human and Environmental Sciences, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan. kojima@giptc.shinshu-u.ac.jp
Shioiri
Hidenari
H
Nogawa
Masahiro
M
Nozue
Masayuki
M
Matsumoto
Daisuke
D
Wada
Asami
A
Saiki
Yumi
Y
Kiguchi
Kenji
K