title: In vitro differentiation and mineralization of human dental pulp cells induced by dentin extract.
abstract: In this study, the progenitor cells isolated from the human dental pulp were used to study the effects of ethylenediaminetetraacetic acid-soluble dentin extract (DE) on their differentiation and mineralization to better understand tissue injury and repair in the tooth. Mineralization of the matrix was increasingly evident at 14, 21, and 28 d after treatment with a mineralization supplement (MS) (ascorbic acid [AA], beta-glycerophosphate [beta-GP]) and MS + DE. Real-time polymerase chain reaction results showed type I collagen upregulation after the addition of MS + DE at 7 d. Alkaline phosphatase was downregulated after the mineralization became obvious at 14 d. Bone sialoprotein was shown to be upregulated in the mineralized cell groups at all time points and dentin sialophosphoprotein after 7 d. Core binding factor a 1 was upregulated by the treatment of MS and DE at 7, 14, and 21 d. These results indicated that the MS of AA, beta-GP, and DE synergistically induced cell differentiation of pulp progenitor cells into odontoblast-like cells and induced in vitro mineralization.
authors.affiliation: Department of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, 1011 North University, Ann Arbor, Michigan 48109-1078, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Taocong
authors.initials: T
authors.lastname: Jin
authors.forename: Helena H
authors.initials: HH
authors.lastname: Ritchie
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Smith
authors.forename: Brian H
authors.initials: BH
authors.lastname: Clarkson
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002113:Calcification, Physiologic; D002454:Cell Differentiation / Q000502:physiology*; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D003731:Dental Caries / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology* / Q000502:physiology*; D003804:Dentin / Q000737:chemistry / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism; D006801:Humans; D058574:Integrin-Binding Sialoprotein; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D013552:Swine; D014020:Tissue Extracts / Q000378:metabolism*; D014815:Vitamins / Q000378:metabolism
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D005994:Glycerophosphates; C546871:IBSP protein, human; D058574:Integrin-Binding Sialoprotein; D011498:Protein Precursors; D012795:Sialoglycoproteins; D014020:Tissue Extracts; D014815:Vitamins; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid; C031463:beta-glycerophosphoric acid