A cuticle protein gene from the Japanese oak silkmoth, Antheraea yamamai: gene structure and mRNA expression.
A cuticle protein gene, AyCP12, from the Japanese oak silkmoth, Antheraea yamamai, was isolated and characterized. The gene spans 1107 bp and consists of one intron and two exons coding for a 112 amino acid polypeptide with a predicted molecular mass of 12,163 Da and a pI of 4.4. The AyCP12 protein contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the AyCP12 cDNA is most homologous to another silkmoth, A. pernyi, cuticle protein ApCP13 (82% protein sequence identity). Northern blot analysis revealed that AyCP12 showed the epidermis-specific expression.
Kim
Bo Yeon
BY
Department of Sericulture and Entomology, Miryang National University, 627-130, Miryang, Korea.
Park
Nam Sook
NS
Jin
Byung Rae
BR
Lee
Bong Hee
BH
Seong
Su Il
SI
Hwang
Jae Sam
JS
Chang
Jong Su
JS
Lee
Sang Mong
SM
D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry* / Q000302:isolation & purification; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D019476:Insect Proteins; D012333:RNA, Messenger; C047663:cuticle proteins, insects