Production of cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene (toluene cis glycol) by Rhodococcus sp. MA 7249.
An attractive method for producing cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene (toluene cis glycol) was developed employing a cis dihydrodiol dehydrogenase "deficient" strain of Rhodococcus (MA 7249). The toluene cis glycol produced was found to have optical rotations of [alpha]D25 = +25.8 (c 0.45, CH3OH) and +72.8 (c 0.42, CHCl3) which indicated an absolute configuration of (1S,2R) when compared with previously published values. When cultivated in laboratory fermentor in the presence of toluene vapors, MA 7249 reached a toluene cis glycol concentration up to 18 g/l in 110 h. Culture MA 7249 also accumulated cis (1S,2R) dihydrodiols from dihydronaphthalene, biphenyl, chlorobenzene, and styrene.
Chartrain
M
M
Department of Bioprocess, Merck Research Laboratories, RY 80Y-105, Merck and Co., PO Box 2000, Rayway, NJ 07065, USA.
Ikemoto
N
N
Taylor
C
C
Stahl
S
S
Sandford
V
V
Gbewonyo
K
K
Chirdo
C
C
Maxwell
C
C
Osoria
J
J
Buckland
B
B
Greasham
R
R