Overexpression and purification of microbial pro-transglutaminase from Streptomyces cinnamoneum and in vitro processing by Streptomyces albogriseolus proteases.
Processing and activation of the precursor of an extracellular Streptomyces transglutaminase were achieved by using three Streptomyces proteases (SAM-P20, SAM-P26 and SAM-P45), all of which are widely distributed in Streptomyces. The use of these proteases would allow us to develop a production process for the active form of this enzyme in recombinant bacteria.
Taguchi
Seiichi
S
School of Agriculture, Meiji University, 1-1-1 Higashi-mita, Tama-ku, Kawasaki, Kamagawa 214-8571, Japan. staguchi@isc.meiji.ac.jp
Arakawa
Kumiko
K
Yokoyama
Keiichi
K
Takehana
Shino
S
Takagi
Hiroshi
H
Momose
Haruo
H