title: Evaluation of immunity and protective efficacy of a dengue-3 pre-membrane and envelope DNA vaccine in Aotus nancymae monkeys.
abstract: A dengue (DEN) virus type 3 DNA vaccine expressing pre-membrane and envelope genes was tested for immunogenicity and protective efficacy in Aotus monkeys. Five of six vaccinated animals demonstrated moderate DEN-specific antibody responses as measured by ELISA and virus neutralization in vitro. By contrast, none of the six control animals developed detectable anti-DEN antibodies. When five vaccinated animals were challenged with live DEN-3 virus and viremia determined by PCR amplification of viral RNA in serum samples, one animal was completely protected and two were partially protected as indicated by a decrease in mean days of viremia. The results demonstrate the ability of the DEN-3 DNA vaccine to elicit a neutralizing antibody response and to partially protect against live virus challenge. These findings support the inclusion of this construct in a tetravalent DNA vaccine.
authors.affiliation: Naval Medical Research Center Detachment, Unit 3800 American Embassy, APO AA 34031-3800, USA.
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Blair
authors.forename: Tadeusz J
authors.initials: TJ
authors.lastname: Kochel
authors.forename: Kanakatte
authors.initials: K
authors.lastname: Raviprakash
authors.forename: Carolina
authors.initials: C
authors.lastname: Guevara
authors.forename: Milagros
authors.initials: M
authors.lastname: Salazar
authors.forename: Shuenn-Jue
authors.initials: SJ
authors.lastname: Wu
authors.forename: James G
authors.initials: JG
authors.lastname: Olson
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Porter
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D016645:Aotidae; D003715:Dengue / Q000276:immunology* / Q000517:prevention & control*; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009500:Neutralization Tests; D012367:RNA, Viral / Q000097:blood; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D012367:RNA, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines