Acetaldehyde addition and pre-adaptation to the stressor together virtually eliminate the ethanol-induced lag phase in Saccharomyces cerevisiae.
OBJECTIVE
To show that the ethanol-induced lag phase in yeast can be almost eliminated by combining pre-adaptation with acetaldehyde supplementation.


RESULTS
Pre-adaptation to noninhibitory concentrations of ethanol and supplementation of unadapted cultures with acetaldehyde each separately reduced the lag phase of ethanol-inhibited cultures by c. 70%. By combining the two methods the ethanol-induced lag phase was virtually eliminated (90% reduction in lag time).


CONCLUSIONS
Pre-adaptation to ethanol and acetaldehyde supplementation appear to promote yeast growth through different mechanisms, which are additive when combined.


CONCLUSIONS
The combination of the above procedures is a potentially powerful tool for reducing the lag of stressed cultures, which may have practical applications: e.g. in reducing the lag of yeasts inoculated into lignocellulosic hydrolysates employed in fuel ethanol production.
Vriesekoop
F
F
Institute for Food and Crop Science, School of Science and Engineering, University of Ballarat, Ballarat, Vic., Australia. f.vriesekoop@ballarat.edu.au
Pamment
N B
NB
D000079:Acetaldehyde / Q000494:pharmacology*; D000222:Adaptation, Physiological / Q000502:physiology*; D002453:Cell Cycle / Q000187:drug effects* / Q000502:physiology; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D014158:Transcription, Genetic
D000431:Ethanol; D000079:Acetaldehyde