title: A cytoplasmic xylanase (XynX) of Aeromonas caviae ME-1 is released from the cytoplasm to the periplasm by osmotic downshock.
abstract: Aeromonas caviae ME-1 is a multiple xylanase-producing gram-negative bacterium which was isolated from the gut contents of a wild silkworm, Samia cynthia pryeri. One of the xylanases produced by A. caviae ME-1, XynX (38 kDa, family 10 xylanase), hydrolyzes xylan to xylobiose and xylotetraose as final degradation products. Generally, xylanases are extracellular or cell surface enzymes. However, XynX is not exported to the extracellular fluid by A. caviae ME-1 and an Escherichia coli transformant harboring the xynX gene. In this study, we investigated the intracellular localization of XynX in A. caviae ME-1 and an E. coli transformant. XynX was found in the cytoplasm when the cells were grown under normal culture conditions. However, XynX was released from the cytoplasm to the periplasm during osmotic downshock. This release of XynX in the E. coli transformant was blocked in the presence of gadolinium chloride, which has been reported to be an inhibitor of bacterial mechanosensitive channels.
authors.affiliation: Department of Biotechnology, United Graduate School Agricultural Science, Department of Biotechnology, Faculty of Agriculture, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Usui
authors.forename: Tohru
authors.initials: T
authors.lastname: Suzuki
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Akisaka
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kawai