title: Prevalence of metallo-beta lactamase (MBL) producing Pseudomonas spp. and Acinetobacter spp. in a tertiary care hospital in India.
abstract: OBJECTIVE
  Gram-negative bacteria with acquired metallo-beta lactamases production have been increasingly reported in some countries, necessitating their detection. We determined the occurrence of acquired metallo-beta lactamases (MBL) producing bacteria in our teaching hospital.
  
  
  METHODS
  The method employed was a rapid technique using a disk of imipenem plus 750 microg of EDTA, on Mueller-Hinton plate which was supplemented with 70 microg/ml of zinc sulfate, to differentiate MBL producing Pseudomonads and Acinetobacter.
  
  
  RESULTS
  Out of 200 isolates tested, 7.5% of our Pseudomonads and Acinetobacter were shown to be MBL producers.
  
  
  CONCLUSIONS
  Though MBL prevalence is still low in our hospital set up, screening for MBL among Pseudomonas and Acinetobacter isolates should be routinely done in laboratory.
authors.affiliation: Department of Microbiology, Government Medical College and Hospital, 1154 A, Sec-32 B, Chandigarh 160030, India. varshagupta_99@yahoo.com
authors.forename: Varsha
authors.initials: V
authors.lastname: Gupta
authors.forename: Priya
authors.initials: P
authors.lastname: Datta
authors.forename: Jagdish
authors.initials: J
authors.lastname: Chander
mesh_terms: D000150:Acinetobacter / Q000201:enzymology* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology; D006761:Hospitals; D006801:Humans; D007194:India / Q000453:epidemiology; D015995:Prevalence; D011549:Pseudomonas / Q000201:enzymology* / Q000302:isolation & purification; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases