title: In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecule PCR and cell-free protein synthesis.
abstract: A mutant library of Burkholderia cepacia lipase KWI-56 was constructed on microplates by a cell-free process and tested with a chromogenic assay. This high-throughput construction system can be used to screen mutant proteins based on their catalytic activity.
authors.affiliation: Laboratory of Molecular Biotechnology, Graduate School of Biological & Agricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Koga
authors.forename: Kouei
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Junhao
authors.initials: J
authors.lastname: Yang
authors.forename: Hideo
authors.initials: H
authors.lastname: Nakano
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Yamane