Effects of antimicrobial components of essential oils on growth of Bacillus cereus INRA L2104 in and the sensory qualities of carrot broth.
The possible use of antimicrobials from seven plant essential oils as food preservatives was studied by examining their effects on the growth kinetics of activated Bacillus cereus INRA L2104 spores inoculated into tyndallized carrot broth. The effects of various concentrations of borneol, carvacrol, cinnamaldehyde, eugenol, menthol, thymol, and vanillin were determined. Five microliters of cinnamaldehyde, 15 microl of carvacrol, or 30 mg of thymol per 100 ml of inoculated carrot broth completely inhibited bacterial growth for more than 60 days at 16 degrees C. Lower concentrations of the three antimicrobials prolonged the lag phase and reduced both the exponential growth rate and the final population densities of cultures. The study of the sensory characteristics of the supplemented broths suggested that low concentration of cinnamaldehyde enhanced the taste of carrot broth, and that it did not have any adverse effect on the taste and smell of carrot broth at concentrations less than 6 microl 100 ml(-1).
Valero
M
M
Departamento de Producción Vegetal y Microbiología, Escuela Politécnica Superior de Orihuela, Universidad Miguel Hernández-Campus de Orihuela, Alicante, Spain. m.valero@umh.es
Giner
M J
MJ
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001409:Bacillus cereus / Q000254:growth & development* / Q000502:physiology; D003257:Consumer Product Safety; D018552:Daucus carota / Q000382:microbiology*; D004305:Dose-Response Relationship, Drug; D005511:Food Handling / Q000379:methods; D005519:Food Preservation / Q000379:methods*; D006801:Humans; D007700:Kinetics; D009812:Odorants / Q000032:analysis; D009822:Oils, Volatile / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000254:growth & development; D013649:Taste; D013696:Temperature; D013997:Time Factors
D000900:Anti-Bacterial Agents; D009822:Oils, Volatile