[Relationship of microtubule and [Ca2+]cyt in wheat mesophyll protoplast].
By using immunofluorescence labeling technique and incubating the Fluo-3AM of Ca2+ fluorescein, wheat (Triticum aestivum) cultivars Lovrin 10 was selected as mesophyll protoplast material in this paper for studying the relationship between the pattern of microtubule and [Ca2+]cyt. Laser scanning confocal microscopy(LSCM) was used and pharmacologic examination was set. The results indicated there was a connection between calcium signaling and microtubule organization in wheat mesophyll protoplast. When the cytosolic calcium concentration distinctly ascends, the pattern of microtubule showed depolymerization; Depolymerization of microtubule could enhance extracellar calcium afflux.
Liu
Gang
G
College of life science, Agricultural University of Hebei, Baoding 071001.
Wang
Dong Mei
DM
D001692:Biological Transport / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D018613:Microscopy, Confocal; D008870:Microtubules / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism*
D002118:Calcium