The plant nuclear envelope protein MAF1 has an additional location at the Golgi and binds to a novel Golgi-associated coiled-coil protein.
Tomato MAF1 (LeMAF1) is a plant-specific, nuclear envelope (NE)-associated protein. It is the founding member of a group of WPP domain-containing, NE-associated proteins. This group includes the Arabidopsis WPP family, which is involved in cell division, as well as plant RanGAPs. In addition to its NE localization, LeMAF1 accumulates in speckles in the cytoplasm. Here, we show that the LeMAF1-containing speckles are components of the Golgi apparatus. A novel tomato coiled-coil protein was identified that specifically binds to LeMAF1. Tomato WPP domain-associated protein (LeWAP) interacts in yeast and in vitro through its coiled-coil domain with several WPP-domain containing proteins, including AtRanGAP1 and the WPP family (LeMAF, WPP1 and WPP2). Like LeMAF1, LeWAP is localized at the Golgi. Moreover, we present data showing that Arabidopsis WAP is necessary for the existence of a multi-protein complex containing WPP2.
Patel
Shalaka
S
Plant Biotechnology Center and Department of Plant Cellular and Molecular Biology, The Ohio State University, 244 Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210-1002, USA.
Brkljacic
Jelena
J
Gindullis
Frank
F
Rose
Annkatrin
A
Meier
Iris
I
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism; D015153:Blotting, Western; D002455:Cell Division; D020690:GTPase-Activating Proteins / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000032:analysis; D018551:Solanum lycopersicum / Q000378:metabolism*; D036961:Matrix Attachment Region Binding Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009685:Nuclear Envelope / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D014026:Nicotiana / Q000166:cytology; D020798:Two-Hybrid System Techniques
C466201:AT3G63130 protein, Arabidopsis; D029681:Arabidopsis Proteins; D020690:GTPase-Activating Proteins; D036961:Matrix Attachment Region Binding Proteins; D008565:Membrane Proteins; D010940:Plant Proteins; C501409:WPP1 protein, Arabidopsis; C501410:WPP2 protein, Arabidopsis; D049452:Green Fluorescent Proteins