Toxic serum factor long after single exposure to organophosphate; a new approach for biomonitoring.
One of the major limitations of current methods of biological detection of exposure to hazardous environmental agents is their inability to detect long-term exposures. In the current study we examined the potential of a new bioassay based on the hypothesis that serum of exposed individuals contains a toxic factor(s) produced by an affected cell/tissue. The procedure included exposure of neuronal PC12 cell cultures to sera of rats treated once with the organophosphate chlorpyrifos. Samples taken 4 weeks after chlorpyrifos exposure reduced nerve growth factor (NGF)-induced neurite outgrowth by 40%. This effect lasted 6 weeks after treatment, whereas motor activity and cholinesterase activity returned to normal levels within 1 week. These results demonstrate the potential of the proposed method to detect environmental exposures long after they have occurred.
Berman-Shlomovich
Tamar
T
Department of Pharmacology, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel.
Wormser
Uri
U
Brodsky
Berta
B
D000818:Animals; D001685:Biological Factors / Q000097:blood*; D004390:Chlorpyrifos / Q000633:toxicity*; D002802:Cholinesterases / Q000097:blood; D004784:Environmental Monitoring / Q000379:methods*; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum
D001685:Biological Factors; D002802:Cholinesterases; D004390:Chlorpyrifos