Rapid identification of Burkholderia pseudomallei in blood culture supernatants by a coagglutination assay.
In total, 309 blood culture supernatants were tested for the presence of Burkholderia pseudomallei antigen using an in-house coagglutination test prepared by sensitising Cowan I staphylococcal cells with B. pseudomallei polyclonal antiserum. The coagglutination test gave a sensitivity, specificity, positive predictive value and negative predictive value of 100% in comparison with blood culture. A subset of 102 supernatants was also tested for B. pseudomallei antigen using a monoclonal antibody-based latex agglutination test. The sensitivity, specificity, positive predictive value and negative predictive values of this test were 100%, 90%, 75% and 100%, respectively.
Jesudason
M V
MV
Department of Clinical Microbiology, Christian Medical College, Vellore, India. micro@cmcvellore.ac.in
Balaji
V
V
Sirisinha
S
S
Sridharan
G
G
D000372:Agglutination Tests; D000942:Antigens, Bacterial / Q000097:blood*; D001769:Blood / Q000382:microbiology*; D016957:Burkholderia pseudomallei / Q000276:immunology* / Q000302:isolation & purification*; D006801:Humans; D007841:Latex Fixation Tests; D008554:Melioidosis / Q000175:diagnosis* / Q000382:microbiology; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial