title: Cytoprotective effect of phloroglucinol on oxidative stress induced cell damage via catalase activation.
abstract: We investigated the cytoprotective effect of phloroglucinol, which was isolated from Ecklonia cava (brown alga), against oxidative stress induced cell damage in Chinese hamster lung fibroblast (V79-4) cells. Phloroglucinol was found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydrogen peroxide (H(2)O(2)), hydroxy radical, intracellular reactive oxygen species (ROS), and thus prevented lipid peroxidation. As a result, phloroglucinol reduced H(2)O(2) induced apoptotic cells formation in V79-4 cells. In addition, phloroglucinol inhibited cell damage induced by serum starvation and radiation through scavenging ROS. Phloroglucinol increased the catalase activity and its protein expression. In addition, catalase inhibitor abolished the protective effect of phloroglucinol from H(2)O(2) induced cell damage. Furthermore, phloroglucinol increased phosphorylation of extracellular signal regulated kinase (ERK). Taken together, the results suggest that phloroglucinol protects V79-4 cells against oxidative damage by enhancing the cellular catalase activity and modulating ERK signal pathway.
authors.affiliation: Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju-si 690-756, Korea.
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Lee
authors.forename: Sungwook
authors.initials: S
authors.lastname: Chae
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Myung Sun
authors.initials: MS
authors.lastname: Jung
authors.forename: Young Min
authors.initials: YM
authors.lastname: Ham
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Baik
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism*; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D019610:Cytoprotection / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006861:Hydrogen Peroxide; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010276:Parasympatholytics / Q000494:pharmacology*; D000459:Phaeophyceae / Q000737:chemistry; D010696:Phloroglucinol / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D010276:Parasympatholytics; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D010696:Phloroglucinol; D002374:Catalase; D048049:Extracellular Signal-Regulated MAP Kinases