Cloning of the chicken RNA polymerase I promoter and use for reverse genetics of influenza A viruses in avian cells.
Reverse genetics techniques to rescue influenza viruses have thus far been based on the use of a human polymerase I (PolI) promoter to direct the synthesis of the eight viral RNAs. They can only be used on cells from primate origin due to the species specificity of the PolI promoter. Here we report the cloning of the chicken PolI promoter sequence and the generation of recombinant influenza virus upon transfection of bidirectional PolI/PolII plasmids in avian cells. Potential contributions of this new reverse genetics system in the fields of influenza virus research and influenza vaccine production are discussed.
Massin
Pascale
P
Unité de Génétique Moléculaire des Virus Respiratoires, URA CNRS 1966, Institut Pasteur, 25 rue du Dr. Roux, 75724 PARIS Cedex 15, France.
Rodrigues
Pierre
P
Marasescu
Monica
M
van der Werf
Sylvie
S
Naffakh
Nadia
N
D000818:Animals; D001483:Base Sequence; D001717:Birds; D002460:Cell Line; D002645:Chickens; D009980:Influenza A virus / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012318:RNA Polymerase I / Q000235:genetics*; D011995:Recombination, Genetic
D012318:RNA Polymerase I