Mathematical modeling of mitochondrial adenine nucleotide translocase.
We have developed a mathematical model of adenine nucleotide translocase (ANT) function on the basis of the structural and kinetic properties of the transporter. The model takes into account the effect of membrane potential, pH, and magnesium concentration on ATP and ADP exchange velocity. The parameters of the model have been estimated from experimental data. A satisfactory model should take into account the influence of the electric potential difference on both ternary complex formation and translocation processes. To describe the dependence of translocation constants on electric potential we have supposed that ANT molecules carry charged groups. These groups are shifted during the translocation. Using the model we have evaluated the translocator efficiency and predicted the behavior of ANT under physiological conditions.
Metelkin
Eugeniy
E
A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonosov, Moscow State University, Moscow, Russia. metelkin@insysbio.ru
Goryanin
Igor
I
Demin
Oleg
O
D000244:Adenosine Diphosphate / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D001703:Biophysics / Q000379:methods*; D002384:Catalysis; D019281:Dimerization; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008564:Membrane Potentials; D008928:Mitochondria / Q000201:enzymology*; D000226:Mitochondrial ADP, ATP Translocases / Q000737:chemistry*; D008956:Models, Chemical; D015233:Models, Statistical; D008962:Models, Theoretical; D010085:Oxidative Phosphorylation; D012680:Sensitivity and Specificity; D013816:Thermodynamics
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000226:Mitochondrial ADP, ATP Translocases