Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein.
We describe a novel homogeneous protein-protein interaction assay based on bioluminescence resonance energy transfer (BRET) from mutant firefly luciferase to red fluorescent protein (DsRed). The association of glutathione S-transferase (GST)-luciferase and protein G-DsRed, which was driven by the addition of anti-GST antibody, was successfully monitored by BRET.
Arai
Ryoichi
R
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Nakagawa
Hideyuki
H
Kitayama
Atsushi
A
Ueda
Hiroshi
H
Nagamune
Teruyuki
T