title: Shock wave therapy as an innovative technology in skeletal disorders: study on transmembrane current in stimulated osteoblast-like cells.
abstract: Extracorporeal shock wave treatment (ESWT) is successfully used in various musculoskeletal disorders and pathologies. Despite the increasing use of this kind of therapy, some aspects of its mechanism of action are still unclear. In vitro bone cell behavior under ESWT were previously investigated by the present author and MG63 osteoblast-like cells showed an enhancement in proliferation and in the osteoblast differentiation after therapy with a low-energy flux density. The aim of the present study was to evaluate the effect of ESWT on the permeabilization of cell membrane. We characterized physiological changes in the MG63 associated with ESWT generated by an ESW device and patch clamp recording was performed to study ion channels. Experiments were carried out using the whole-cell recording configuration of the patch-clamp technique and the ionic current measurements were performed on cell samples of ESW treated and control groups. The patch-clamp technique showed the effect of ESWT on the amplitude of transmembrane currents. The treatment with ESW enhanced the transmembrane current as well the voltage dependence of Ca-activated and K channels that mediate these currents: the differences between treated cells and control at 80mV were over 1000 pA (p<0.05). These modifications of ion channels activity positively influence cell proliferation (MTT test, p<0.0001) without interfering with the normal synthesis activity of stimulated osteoblasts.
authors.affiliation: Experimental Surgery Department, Research Institute Codivilla-Putti, Rizzoli Orthopedic Institute, Via de Barbiano 1/10, 4-136 Bologna, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Martini
authors.forename: G
authors.initials: G
authors.lastname: Giavaresi
authors.forename: M
authors.initials: M
authors.lastname: Fini
authors.forename: P
authors.initials: P
authors.lastname: Torricelli
authors.forename: V
authors.initials: V
authors.lastname: Borsari
authors.forename: R
authors.initials: R
authors.lastname: Giardino
authors.forename: M
authors.initials: M
authors.lastname: De Pretto
authors.forename: D
authors.initials: D
authors.lastname: Remondini
authors.forename: G C
authors.initials: GC
authors.lastname: Castellani
mesh_terms: D002452:Cell Count; D002463:Cell Membrane Permeability; D002470:Cell Survival; D002478:Cells, Cultured; D019220:High-Energy Shock Waves; D006801:Humans; D008564:Membrane Potentials; D010006:Osteoblasts / Q000528:radiation effects*; D018408:Patch-Clamp Techniques