Isolation and characterization of a glucose/mannose-specific lectin with stimulatory effect on nitric oxide production by macrophages from the emperor banana.
Emperor banana (Musa basjoo cv. 'Emperor Banana') is a banana cultivar that has not been studied previously. In this study, a glucose/mannose-specific lectin has been purified from the emperor banana by affinity chromatography on Affi-gel blue gel, ion exchange chromatography on Mono S and gel filtration by fast protein liquid chromatography on Superdex 75. This lectin was composed of two identical 15-kDa subunits with N-terminal amino acid sequence similarity to other lectins from other Musa species. Emperor banana lectin stimulated [3H-methyl]-thymidine uptake by mouse splenocytes and nitric oxide production by mouse macrophages. In contrast to Con A, the mitogenic activity of emperor banana lectin toward mouse splenocytes but not its stimulatory effect on nitric oxide production by mouse macrophages could be abrogated by 200 mM glucose. Emperor banana lectin also inhibited proliferation of leukemia cell (L1210) and the activity of HIV-1 reverse transcriptase. In summary, this is the first report of the macrophage-stimulating, antiproliferative and HIV-1 reverse transcriptase inhibiting activities of a banana lectin.
Wong
Jack Ho
JH
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
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T B
TB
D000595:Amino Acid Sequence; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D005938:Glucocorticoids / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D028521:Musa / Q000737:chemistry*; D009569:Nitric Oxide / Q000096:biosynthesis*; D037121:Plant Lectins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D016415:Sequence Alignment; D013154:Spleen / Q000166:cytology
D005938:Glucocorticoids; D037121:Plant Lectins; D009569:Nitric Oxide; D003907:Dexamethasone; D005947:Glucose; D008358:Mannose