Highly dense protein layers confirmed by atomic force microscopy and quartz crystal microbalance.
Protein adsorption on a gold surface is investigated by comparing the results of quartz crystal microbalance method and atomic force microscopy. The adsorption of streptavidin on functional gold surfaces is directly monitored by a quartz crystal microbalance, and confirmed by atomic force microscopy. For this investigation, a modified gold substrate is fabricated to obtain a topographic image of streptavidin molecules. Both methods show a correlation in terms of the highly dense protein single-layer formation, and the modified gold electrode shows a slightly denser protein layer formation because of the difference in substrate geometry as compared with that of a mica surface.
Kim
Jongmin
J
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
Yamasaki
Ryujiroh
R
Park
Jongwan
J
Jung
Hosup
H
Lee
Heayeon
H
Kawai
Tomoji
T