title: Selection of a suitable strain from recombinant Escherichia coli strains with the same genetic structure expressing periplasmic hGM-CSF.
abstract: The selection of a suitable strain among five recombinant Escherichia coli strains with the same genetic structure that expresses the human granulocyte macrophage colony stimulating factor (hGM-CSF) was carried out based on four criteria:growth rate, expression level, plasmid stability and feasibility for protein extraction. There was no significant difference in growth between the five strains, while a suitable expression level, a high plasmid stability and a good feasibility for protein extraction from periplasmic space were observed for one of the recombinant strains. This strain expressed 27% hGM-CSF relative to total proteins and had 96% plasmid stability after 7-d subcultures on an antibiotic-free LB medium.
authors.affiliation: Industrial Fermentation Group, National Research Center for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran.
authors.forename: Seyed Safa-Ali
authors.initials: SS
authors.lastname: Fatemi
authors.forename: Bagher
authors.initials: B
authors.lastname: Yakhchali
authors.forename: Jafar Towfighi
authors.initials: JT
authors.lastname: Darian
authors.forename: Seyed Abbas
authors.initials: SA
authors.lastname: Shojaosadati
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Zomorodipour
authors.forename: Ali Asghar
authors.initials: AA
authors.lastname: Karkhane
authors.forename: Ferdows Rastgar
authors.initials: FR
authors.lastname: Jazii