title: Compact oleic acid in HAMLET.
abstract: HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between alpha-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.
authors.affiliation: Department of Biophysical Chemistry, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. jfast@colorado.edu
authors.forename: Jonas
authors.initials: J
authors.lastname: Fast
authors.forename: Ann-Kristin
authors.initials: AK
authors.lastname: Mossberg
authors.forename: Hanna
authors.initials: H
authors.lastname: Nilsson
authors.forename: Catharina
authors.initials: C
authors.lastname: Svanborg
authors.forename: Mikael
authors.initials: M
authors.lastname: Akke
authors.forename: Sara
authors.initials: S
authors.lastname: Linse
mesh_terms: D006801:Humans; D007768:Lactalbumin / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D019301:Oleic Acid / Q000737:chemistry*; D009829:Oleic Acids / Q000737:chemistry*; D011487:Protein Conformation; D017510:Protein Folding
chemical_list: C506422:HAMLET complex, human; D009829:Oleic Acids; D019301:Oleic Acid; D007768:Lactalbumin