title: Molecular evidence of mixed P. vivax and P. falciparum infections in northern Islamic Republic of Iran.
abstract: This study compared basic microscopy with molecular detection of Plasmodium species. According to thick-film microscopy, 100% of 142 malaria cases in Pars-Abad, Ardebil province, were infected with a single species, P vivax. However, nested polymerase chain reaction (PCR) detected mixed species infections of both P. vivax and P. falciparum in 7.0%. In Mazanderan province, 2/20 blood films were diagnosed with only P. falciparum and 18/20 with only P. vivax. However, nested PCR detected 17/20, 2/20 and 1/20 with P. vivax only, P. falciparum only and mixed species respectively. The unexpected presence of P. falciparum urges prompt investigation and immediate treatment of malaria cases in this region.
authors.affiliation: Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran, Tehran, Islamic Republic of Iran.
authors.forename: S
authors.initials: S
authors.lastname: Zakeri
authors.forename: S
authors.initials: S
authors.lastname: Mamaghani
authors.forename: A A
authors.initials: AA
authors.lastname: Mehrizi
authors.forename: Z
authors.initials: Z
authors.lastname: Shahsavari
authors.forename: A
authors.initials: A
authors.lastname: Raeisi
authors.forename: S
authors.initials: S
authors.lastname: Arshi
authors.forename: N
authors.initials: N
authors.lastname: Dinparast-Djadid
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D002675:Child, Preschool; D004396:Coloring Agents; D005260:Female; D006801:Humans; D007223:Infant; D007492:Iran; D016778:Malaria, Falciparum / Q000150:complications* / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D016780:Malaria, Vivax / Q000150:complications* / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D008297:Male; D008853:Microscopy / Q000592:standards; D008875:Middle Aged; D017720:Molecular Epidemiology / Q000379:methods* / Q000592:standards; D010963:Plasmodium falciparum / Q000235:genetics* / Q000648:ultrastructure; D010966:Plasmodium vivax / Q000235:genetics* / Q000648:ultrastructure; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D011159:Population Surveillance; D015995:Prevalence; D012621:Seasons; D012680:Sensitivity and Specificity; D014504:Urban Health / Q000706:statistics & numerical data
chemical_list: D004396:Coloring Agents