Studies on the interaction mechanism between hexakis(imidazole) manganese(II) terephthalate and DNA and preparation of DNA electrochemical sensor.
The interaction between hexakis(imidazole) manganese(II) terephthalate ([Mn(Im)(6)](teph).4H(2)O) and salmon sperm DNA in 0.2M pH 2.30 Britton-Robinson buffer solution was studied by fluorescence spectroscopy and cyclic voltammetry. Increasing fluorescence was observed for [Mn(Im)(6)](2+) with DNA addition, while quenching fluorescence phenomenon appeared for EB-DNA system when [Mn(Im)(6)](2+) was added. There were a couple quasi-reversible redox peaks of [Mn(Im)(6)](2+) from the cyclic voltammogram on the glassy carbon electrode. The peak current of [Mn(Im)(6)](2+) decreased with positive shift of the formal potential in the presence of DNA compared with that in the absence of DNA. All the experimental results indicate that [Mn(Im)(6)](2+) can bind to DNA mainly by intercalative binding mode. The binding ratio of the DNA-[Mn(Im)(6)](2+) association complex is calculated to be 1:1 and the binding constant is 4.44x10(3) M(-1). By using [Mn(Im)(6)](teph).4H(2)O as the electrochemical hybridization indicator, the DNA electrochemical sensor was prepared by covalent interaction and the selectivity of ssDNA modified electrode were described. The results demonstrate the use of electrochemical DNA biosensor in the determination of complementary ssDNA.
Zhang
Shu-Sheng
SS
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Zhengzhou Road 53, Qingdao, Shandong 266042, PR China. shushzhang@126.com
Niu
Shu-Yan
SY
Qu
Bin
B
Jie
Gui-Fen
GF
Xu
Hua
H
Ding
Cai-Feng
CF
D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004563:Electrochemistry; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008345:Manganese / Q000737:chemistry / Q000494:pharmacology*; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology*; D010795:Phthalic Acids / Q000737:chemistry / Q000494:pharmacology; D012474:Salmon; D013050:Spectrometry, Fluorescence
D009942:Organometallic Compounds; D010795:Phthalic Acids; D008345:Manganese; C011363:terephthalic acid; D004247:DNA