Flavonoids purified from Rhus verniciflua Stokes actively inhibit cell growth and induce apoptosis in human osteosarcoma cells.
Many studies have suggested that dietary flavonoids are anticancer agents that induce the apoptosis of cancer cells. However, the effects of flavonoids on the induction of apoptosis in osteosarcoma cells are unclear. Previously, a flavonoid fraction, consisting mainly of protocatechuic acid, fustin, fisetin, sulfuretin, and butein, herein named RCMF (the RVS chloroform-methanol fraction), was prepared from a crude acetone extract of Rhus verniciflua Stokes (RVS). This study evaluated the effects of RCMF on the proliferation and apoptosis using human osteosarcoma (HOS) cells. The mechanism of growth inhibition of the HOS cells by the flavonoid fraction, RCMF, was also assessed. The results demonstrated that RCMF exhibited sensitive growth inhibition and induced apoptosis in HOS cells. PARP cleavage was closely associated with the RCMF-induced apoptosis of the HOS cells. Furthermore, the activation of caspase 8 and Bax, the inhibition of Bcl-2 expression, and the release of cytochrome c are believed to be involved in the RCMF-mediated apoptosis. Collectively, these findings suggest that RCMF is an agent which may be capable of inducing sensitive growth inhibition and apoptosis in HOS cells.
Jang
Hyon-Seok
HS
Department of Dentistry, Oral and Maxillofacial Surgery, College of Medicine, Korea University, Ansan 425-020, Korea.
Kook
Sung-Ho
SH
Son
Young-Ok
YO
Kim
Jong-Ghee
JG
Jeon
Young-Mi
YM
Jang
Yong-Suk
YS
Choi
Ki-Choon
KC
Kim
Ju
J
Han
Seong-Kyu
SK
Lee
Kyung-Yeol
KY
Park
Byung-Keon
BK
Cho
Nam-Pyo
NP
Lee
Jeong-Chae
JC
D016588:Anticarcinogenic Agents / Q000302:isolation & purification / Q000633:toxicity*; D017209:Apoptosis; D001859:Bone Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005419:Flavonoids / Q000302:isolation & purification / Q000633:toxicity*; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D012516:Osteosarcoma / Q000473:pathology; D010936:Plant Extracts / Q000633:toxicity; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D029045:Rhus / Q000737:chemistry
D016588:Anticarcinogenic Agents; D005419:Flavonoids; D010936:Plant Extracts; D011065:Poly(ADP-ribose) Polymerases