A single-chain fragment variable recombinant antibody against F5 fimbria of enterotoxigenic Escherichia coli inhibits agglutination of horse red blood cells induced by F5 protein.
Bovine colibacillosis caused by enterotoxigenic Escherichia coli (ETEC) is a worldwide problem. Adhesion of ETEC to intestinal cell receptors mediated by the surface protein F5 fimbriae is the initial step in the establishment of colibacillosis. Prevention of ETEC F5(+) adhesion to enterocytes protects newborn calves against collibacillosis. On the enterocytes, the F5 fimbriae bind to a ganglioside that is also found on horse red blood cells. Thus, the presence of F5 fimbriae induces haemagglutination, which is useful as an indicator in a functional assay system. In this study, recombinant anti-F5 scFv antibody fragment produced in E. coli HB2151 reacted with F5 fimbriae in ELISA and Western immunoblot, and prevented haemagglutination induced by the binding of the F5 fimbriae to its natural host receptors on horse red blood cells. Given the ease with which recombinant antibodies can be mass-produced, the presently described scFv may hold promise as a prophylactic agent for colibacillosis.
Bhaskaran
S
S
Department of Veterinary Pathobiology, Texas Veterinary Medical Center, Texas A&M University, College Station, TX 77843, USA.
Jay
C M
CM
Berghman
L R
LR
Wagner
G G
GG
Waghela
S D
SD
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001483:Base Sequence; D002460:Cell Line; D004912:Erythrocytes / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000037:antagonists & inhibitors* / Q000502:physiology; D036781:Fimbriae Proteins / Q000037:antagonists & inhibitors* / Q000502:physiology; D006384:Hemagglutination / Q000502:physiology*; D006736:Horses; D008969:Molecular Sequence Data; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D036781:Fimbriae Proteins