Distinction of Leu and Ile Using a Ruthenium(II) complex by MALDI-LIFT-TOF/TOF-MS analysis.
The novel N-terminal labeling method using a ruthenium(II) complex derivative characteristically indicated a(n) and d(n) (N-terminal) fragment ions in high sensitivity by MS/MS analysis (MALDI-LIFT or ESI-CID). Although these fragment ions depended on a fragmentation process by MS/MS analytical methods to some degree, each case indicated similar side-chain cleavage patterns. The labeling method allows accurate distinction of amino acid residues by MS/MS analysis even if the residues are structural isomers such as leucine and isoleucine. The method was applied to long-chain peptides and provided easy and rapid N-terminal sequencing.
Ito
Akihiro
A
Department of Macromolecular Science and Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
Okamura
Taka-Aki
TA
Yamamoto
Hitoshi
H
Ueyama
Norikazu
N
Ake
Kojiro
K
Masui
Ryoji
R
Kuramitsu
Seiki
S
Tsunasawa
Susumu
S
D007532:Isoleucine / Q000737:chemistry*; D007930:Leucine / Q000737:chemistry*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D009942:Organometallic Compounds; D007532:Isoleucine; D012428:Ruthenium; D007930:Leucine