Proteomic analysis of the excretory-secretory proteins of the Trichinella spiralis L1 larva, a nematode parasite of skeletal muscle.
Trichinella spiralis is an intracellular nematode parasite of mammalian skeletal muscle. Infection of the muscle cell leads to the formation of a host-parasite complex that results in profound alterations to the host cell and a re-alignment of muscle-specific gene expression. The role of parasite excretory-secretory (ES) proteins in mediating these effects is currently unknown, largely due to the difficulty in identifying and assigning function to individual proteins. In this study, a global proteomics approach was used to analyse the ES proteins from T. spiralis muscle larvae. Following 2-DE of ES proteins,MALDI-TOF-MS and LC-MS/MS were used to identify the peptide spots. Specific Trichinella EST databases were assembled and used to analyse the data. Despite the current absence of a Trichinella genome-sequencing project, 43 out of 52 protein spots analysed were identified and included the major secreted glycoproteins. Other novel proteins were identified from matches with sequences in the T. spiralis database. Our results demonstrate the value of proteomics as a tool for the identification of Trichinella ES proteins and in the study of the molecular mechanism underpinning the formation of the host-parasite complex during Trichinella infections.
Robinson
Mark W
MW
School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, UK.
Connolly
Bernadette
B
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D020224:Expressed Sequence Tags; D005260:Female; D006031:Glycosylation; D015801:Helminth Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007814:Larva; D013058:Mass Spectrometry; D051379:Mice; D008813:Mice, Inbred ICR; D018482:Muscle, Skeletal / Q000469:parasitology; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017160:Trichinella spiralis / Q000737:chemistry / Q000254:growth & development*; D014235:Trichinellosis
D015801:Helminth Proteins