Inducible production of alcohol oxidase and catalase in a pectin medium by Thermoascus aurantiacus IFO 31693.
Thermoascus aurantiacus showed the best growth on medium containing pectin as a carbon source. The enzyme involved in the production of catalase in the fungus was alcohol oxidase. Formaldehyde dehydrogenase and formate dehydrogenase, in addition to alcohol oxidase and catalase, were detected in the cells grown on pectin. Alcohol oxidase was alkali resistant (pH 7 to 11), and was comparatively heat stable (55 degrees C).
Ko
Hee-Sun
HS
Department of Bioresources Science, Graduate School of Science and Technology, Chiba University, 648 Matsudo, Matsudo-city, Chiba 271-8510, Japan.
Fujiwara
Hidenori
H
Yokoyama
Yuichi
Y
Ohno
Nobuko
N
Amachi
Seigo
S
Shinoyama
Hirofumi
H
Fujii
Takaaki
T
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis*; D000445:Aldehyde Oxidoreductases / Q000096:biosynthesis*; D019149:Bioreactors / Q000382:microbiology*; D002374:Catalase / Q000096:biosynthesis*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000378:metabolism; D032641:Eurotiales / Q000201:enzymology*; D005560:Formate Dehydrogenases / Q000096:biosynthesis*; D010368:Pectins / Q000378:metabolism*
D003470:Culture Media; D010368:Pectins; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D002374:Catalase; D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent