title: Myocardial homing of nonmobilized peripheral-blood CD34+ cells after intracoronary injection.
abstract: Granulocyte--colony-stimulating factor administered for autologous hematopoietic stem cell isolation from blood may favor restenosis in patients implanted after acute myocardial infarction (AMI). We therefore tested the isolation of peripheral-blood CD34+ cells without mobilization in six patients with AMI. After large-volume cytapheresis and positive CD34+ cell selection, 3.6 to 27.6 million CD34+ cells were obtained. We performed intra-coronary implantation of these cells and recorded no restenosis or arrhythmia. We used positron emission tomography (PET) to assess myocardial-labeled CD34+ cell homing, which accounted for 5.5% of injected cells 1 hour after implantation. In conclusion, large amounts of CD34+ cells, in the range reported in previous studies, can be obtained from nonmobilized peripheral blood. PET with [18F]-fluorodeoxyglucose cell labeling is an efficient imaging method for homing assessment.
authors.affiliation: Department of Nuclear Medicine and PET/Biomedical Cyclotron Unit, Hôpital Universitaire Erasme, Université Libre de Bruxelles, Brussels, Belgium. dblockle@ulb.ac.be
authors.forename: Didier
authors.initials: D
authors.lastname: Blocklet
authors.forename: Michel
authors.initials: M
authors.lastname: Toungouz
authors.forename: Guy
authors.initials: G
authors.lastname: Berkenboom
authors.forename: Micheline
authors.initials: M
authors.lastname: Lambermont
authors.forename: Philippe
authors.initials: P
authors.lastname: Unger
authors.forename: Nicolas
authors.initials: N
authors.lastname: Preumont
authors.forename: Eric
authors.initials: E
authors.lastname: Stoupel
authors.forename: Dominique
authors.initials: D
authors.lastname: Egrise
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Degaute
authors.forename: Michel
authors.initials: M
authors.lastname: Goldman
authors.forename: Serge
authors.initials: S
authors.lastname: Goldman
mesh_terms: D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism*; D002465:Cell Movement; D023903:Coronary Restenosis; D003331:Coronary Vessels / Q000502:physiology; D005240:Feasibility Studies; D005260:Female; D005500:Follow-Up Studies; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000628:therapy*; D009206:Myocardium / Q000648:ultrastructure*; D049268:Positron-Emission Tomography / Q000379:methods; D011877:Radionuclide Imaging / Q000379:methods
chemical_list: D018952:Antigens, CD34