title: Affinity identification of delta-opioid receptors using latex nanoparticles.
abstract: Three types of latex nanoparticles carrying naltrindole (NTI) derivatives were synthesized as probes for the affinity isolation of their binding proteins including the delta-opioid receptor. The effect of the attachment of NTI to different positions on the linker was investigated. Only latex nanoparticles in which the NTI derivative was linked through the phenol group were useful for isolating the recombinant delta-opioid receptor solubilized from CHO cell membrane. These latex nanoparticles could be a useful tool for investigations of the pharmacological activity of NTI.
authors.affiliation: Frontier Collaborative Research Center and Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuda, Yokohama 226-8501, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hatakeyama
authors.forename: Haruma
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Handa
mesh_terms: D000818:Animals; D016466:CHO Cells; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D006224:Cricetinae; D003600:Cytosol / Q000378:metabolism; D004745:Enkephalins / Q000737:chemistry; D007840:Latex / Q000737:chemistry*; D008956:Models, Chemical; D009271:Naltrexone / Q000031:analogs & derivatives* / Q000737:chemistry; D049329:Nanostructures; D011485:Protein Binding; D011957:Receptors, Opioid / Q000737:chemistry; D017465:Receptors, Opioid, delta / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D004745:Enkephalins; D007840:Latex; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D011994:Recombinant Proteins; D009271:Naltrexone; C055382:naltrindole