2-hydrazino-1-methylpyridine: a highly sensitive derivatization reagent for oxosteroids in liquid chromatography-electrospray ionization-mass spectrometry.
A derivatization reagent, 2-hydrazino-1-methylpyridine, was developed for the liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) of oxosteroids. The reagent quantitatively reacted with oxosteroids at 60 degrees C within 1h and the resulting derivatives of the mono-oxosteroids provided a 70-1600-fold higher sensitivity compared to intact steroids. However, HMP was unsuitable for di-oxosteroids, such as androstenedione and progesterone. The developed derivatization procedure was applied to the LC-ESI-MS analysis of 5alpha-dihydrotestosterone in human prostate, and allowed the reproducible quantification of nanogram/gram level of the androgen with a 10-mg sample.
Higashi
Tatsuya
T
Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan. higashi@p.kanazawa-u.ac.jp
Yamauchi
Akinori
A
Shimada
Kazutake
K
D000728:Androgens / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D013196:Dihydrotestosterone / Q000032:analysis; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D007664:Ketosteroids / Q000032:analysis*; D008297:Male; D011467:Prostate / Q000737:chemistry; D011470:Prostatic Hyperplasia; D011725:Pyridines / Q000737:chemistry*; D011726:Pyridinium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013739:Testosterone / Q000032:analysis
C506217:2-hydrazino-1-methylpyridine; D000728:Androgens; D006834:Hydrazines; D007664:Ketosteroids; D011725:Pyridines; D011726:Pyridinium Compounds; D013196:Dihydrotestosterone; D013739:Testosterone; C066037:2-fluoro-1-methylpyridinium