In vitro influence of bacterial mixtures on Fusarium verticillioides growth and fumonisin B1 production: effect of seeds treatment on maize root colonization.
OBJECTIVE
Enterobacter cloacae, Microbacterium oleovorans, Pseudomonas solanacearum and Bacillus subtilis were investigated in order to evaluate: (i) the inoculum size of two bacterial mixtures on Fusarium verticillioides growth and fumonisin B1 production in vitro at different water activities and (ii) the efficacy of a seed treatment with the best bacterial mixture on F. verticillioides root colonization in greenhouse studies.


RESULTS
The influence of bacterial mixtures (1 = E. cloacae and M. oleovorans and 2 = P. solanacearum and B. subtilis) to antagonize 13 F. verticillioides strains at different inoculum concentrations (10(8), 10(9) and 10(10) cells ml(-1)) and water activities (0.937, 0.955 and 0.982 aW) were examined. Antibiosis, growth rate and fumonisin B1 production were determined. Bacterial mixture 1 proved to exert the most effective control. Seed treatment with mixture 1 at 10(8) cells ml(-1) had the best inhibitory effect on F. verticillioides root colonization.


CONCLUSIONS
These results suggest that the combination E. cloacae and M. oleovorans has the potential for the biological control of F. verticillioides as a maize seed inoculant.


CONCLUSIONS
The application of this knowledge contributes to prevent the vertical transmission of F. verticillioides.
Cavaglieri
L
L
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina. lcavaglieri@exa.unrc.edu.ar
Orlando
J
J
Etcheverry
M
M
D000898:Antibiosis; D018407:Bacterial Physiological Phenomena; D037341:Fumonisins / Q000378:metabolism*; D005670:Fusarium / Q000254:growth & development*; D010572:Pest Control, Biological; D018517:Plant Roots / Q000382:microbiology; D012988:Soil Microbiology; D003313:Zea mays / Q000382:microbiology*
D037341:Fumonisins; C056933:fumonisin B1