title: Resolution of EBV(+) CNS lymphoma with appearance of CSF EBV-specific T cells.
abstract: Current knowledge of Epstein-Barr virus (EBV)-specific T-cell responses in the cerebrospinal fluid (CSF) of patients with EBV-related lymphoproliferative disease (EBV-LPD) in the central nervous system (CNS) is very limited. Here, we present two recipients of hematopoietic stem cell transplants with EBV-LPD in the CNS. EBV-specific CD8(+) T lymphocytes were detected in CSF and peripheral blood using major histocompatibility complex (MHC) class I multimers loaded with EBV-derived peptides. The appearance of EBV-specific CD8(+) T cells in CSF and blood correlated with neurological improvement and disappearance of EBV-LPD. These observations suggest a role for EBV-specific CD8(+) T cells in the control of EBV-LPD in the CNS.
authors.affiliation: Department of Medical Oncology, Erasmus MC, Rotterdam, The Netherlands.
authors.forename: Janet W
authors.initials: JW
authors.lastname: de Beukelaar
authors.forename: Cornelis
authors.initials: C
authors.lastname: van Arkel
authors.forename: Martin J
authors.initials: MJ
authors.lastname: van den Bent
authors.forename: Mars B
authors.initials: MB
authors.lastname: van't Veer
authors.forename: Gerard J
authors.initials: GJ
authors.lastname: van Doornum
authors.forename: Jan J
authors.initials: JJ
authors.lastname: Cornelissen
authors.forename: Peter A
authors.initials: PA
authors.lastname: Sillevis Smitt
authors.forename: Jan W
authors.initials: JW
authors.lastname: Gratama
mesh_terms: D000368:Aged; D016827:CD8 Antigens / Q000378:metabolism; D016543:Central Nervous System Neoplasms / Q000134:cerebrospinal fluid / Q000601:surgery / Q000821:virology*; D020031:Epstein-Barr Virus Infections / Q000134:cerebrospinal fluid / Q000209:etiology* / Q000821:virology; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects* / Q000379:methods; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D008223:Lymphoma / Q000134:cerebrospinal fluid / Q000601:surgery / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000821:virology*; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism
chemical_list: D016827:CD8 Antigens; C083728:MHC class II transactivator protein; D009687:Nuclear Proteins; D015534:Trans-Activators