[Intervention of cetirizine on monocyte chemoattractant protein-1 in cutaneous inflammation].
OBJECTIVE
To study the intervention of cetirizine on monocyte chemoattractant protein-1 (MCP-1) in different cutaneous inflammation models.


METHODS
Histamine and IFN-gamma stimulated dermal fibroblast cells and HaCaT cells to mimic cutaneous inflammation. Expression of MCP-1 was assessed by means of RT-PCR and ELISA.


RESULTS
Compared with the control group of dermal fibroblast (DF) cells and HaCaT cells, MCP-1 mRNA was significantly upregulated by histamine (10 micromol x L(-1)) and IFN-gamma (20 ng x mL(-1)). The protein secretions of MCP-1 were increased 3.5 fold and 8.4 fold in DF cells, respectively. The similar tendency was observed in HaCaT cells. The enhancing effects of histamine and IFN-gamma on MCP-1 protein production were significantly inhibited by cetirizine (1 and 10 micromol x L(-1)) in DF and HaCaT cells.


CONCLUSIONS
Cetirizine may exert the anti-inflammatory effect of skin via inhibiting MCP-1 expression.
Song
Hong-jie
HJ
Department of Pharmacy, Changhai Hospital, the Second Military Medical Univrersity, Shanghai 200433, China.
Hu
Jin-hong
JH
Huang
Jin
J
Xu
Yan-feng
YF
Jing
Li
L
D002460:Cell Line; D002478:Cells, Cultured; D017332:Cetirizine / Q000494:pharmacology*; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D003872:Dermatitis / Q000378:metabolism; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D039563:Histamine H1 Antagonists, Non-Sedating / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D039563:Histamine H1 Antagonists, Non-Sedating; D012333:RNA, Messenger; D006632:Histamine; D007371:Interferon-gamma; D017332:Cetirizine