title: Amplification and sequencing of Brachyspira spp. specific portions of nox using paraffin-embedded tissue samples from clinical colitis in Austrian pigs shows frequent solitary presence of Brachyspiramurdochii.
abstract: Brachyspira infections are significant causes of enterocolitis in pigs. In order to differentiate pathogenic species (Brachyspira (Br.) hyodysenteriae, Brachyspira pilosicoli) from less pathogenic or non-pathogenic species (Brachyspira intermedia, Brachyspira innocens, Brachyspira murdochii) in paraffin-embedded tissue samples a polymerase chain reaction (PCR) protocol allowing identification of Brachyspira at species level in archival material was developed. This approach was complemented by sequencing of the PCR amplification products. All seven cases presented with clinical and morphological Brachyspira-associated enterocolitis. Br. hyodysenteriae was not identified in any of the cases, while Br. pilosicoli was identified in a single case in conjunction with Br. murdochii. One case each was found positive for Br. innocens and Br. intermedia. Interestingly, the majority of cases presented as single or double infections with Br. murdochii. In some of the pigs other pathogens, like porcine circovirus-2 or Lawsonia intracellularis were present. These observations point at the possibility that under certain conditions even Brachyspira species of low pathogenicity can multiplicate extensively and lead to Brachyspira-associated enterocolitis.
authors.affiliation: Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine, Veterinarplatz 1, A-1210 Vienna, Austria. herbert.weissenboeck@vu-wien.ac.at
authors.forename: Herbert
authors.initials: H
authors.lastname: Weissenböck
authors.forename: Anton
authors.initials: A
authors.lastname: Maderner
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Herzog
authors.forename: Helga
authors.initials: H
authors.lastname: Lussy
authors.forename: Norbert
authors.initials: N
authors.lastname: Nowotny
mesh_terms: D000818:Animals; D001317:Austria / Q000453:epidemiology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003092:Colitis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005784:Gene Amplification; D005838:Genotype; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D013045:Species Specificity; D013144:Spirochaetales / Q000201:enzymology / Q000302:isolation & purification*; D013145:Spirochaetales Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000473:pathology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009247:NADH, NADPH Oxidoreductases; C121789:Nox-2 protein, bacteria