title: Intermediate filament immunohistochemistry of astroglial cells in the leopard gecko, Eublepharis macularius.
abstract: The distribution of intermediate filament molecular markers, glial fibrillary acidic protein (GFAP) and vimentin, has been studied in the central nervous system (CNS) of the adult leopard gecko, Eublepharis macularius. This immunohistochemical study points out the presence of different astroglial cell types. The main pattern is constituted by ependymal radial glia, which have their cell bodies located in the ependymal layer throughout the brain ventricular system. Radial glia proper or radial astrocytes show their cell bodies displaced from the ependymal layer into a periependymal zone and are observed only in the spinal cord. Star-shaped astrocytes are scarce. They are detected in the ventral and lateral regions of the diencephalon and mesencephalon, in the superficial layer of the optic tectum, in the ventral medulla oblongata, and in the ventral and lateral spinal cord. In the different regions of the CNS, the staining intensity appears not to be identical even in the same cellular type. The results reported in the present study show an heterogeneous feature of the astroglial pattern in E. macularius.
authors.affiliation: Department of Biology, University of Bologna, Via Selmi 3, 40126, Italy. maurizio.lazzari@unibo.it
authors.forename: Maurizio
authors.initials: M
authors.lastname: Lazzari
authors.forename: Valeria
authors.initials: V
authors.lastname: Franceschini
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000648:ultrastructure*; D048430:Cell Shape; D002490:Central Nervous System / Q000033:anatomy & histology* / Q000648:ultrastructure*; D019143:Evolution, Molecular; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007382:Intermediate Filaments / Q000737:chemistry; D008116:Lizards / Q000033:anatomy & histology*; D008636:Mesencephalon / Q000166:cytology / Q000648:ultrastructure; D013116:Spinal Cord / Q000166:cytology / Q000648:ultrastructure; D013687:Telencephalon / Q000166:cytology / Q000648:ultrastructure; D014746:Vimentin / Q000032:analysis
chemical_list: D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin