Differentiation of circulating endothelial progenitor cells to a cardiomyogenic phenotype depends on E-cadherin.
Progenitor cells may contribute to cardiac regeneration. Here, we investigated the role of cadherins and integrins for differentiation of human adult circulating endothelial progenitor cells (EPCs) into cardiomyocytes (CM) in a co-culture system. N- and E-cadherin were expressed in EPCs and were localized at the interface between EPCs and CM. Incubation of a blocking antibody against E-cadherin reduced the expression of CM marker protein in EPCs. Blocking antibodies against N- or P-cadherin or the beta1- and beta2-integrins were not effective. These data suggested that cell-to-cell communication mediated by E-cadherin contributes to the acquirement of a cardiomyogenic phenotype of human endothelial progenitor cells.
Koyanagi
Masamichi
M
Molecular Cardiology, Department of Internal Medicine III, University of Frankfurt, Theodor Stern-Kai 7, 60590 Frankfurt, Germany.
Urbich
Carmen
C
Chavakis
Emmanouil
E
Hoffmann
Jörg
J
Rupp
Stefan
S
Badorff
Cornel
C
Zeiher
Andreas M
AM
Starzinski-Powitz
Anna
A
Haendeler
Judith
J
Dimmeler
Stefanie
S
D019138:Antibodies, Blocking / Q000494:pharmacology; D018821:CD18 Antigens / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D015820:Cadherins / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D019012:Integrin beta1 / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology*; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology
D019138:Antibodies, Blocking; D018821:CD18 Antigens; D015820:Cadherins; D019012:Integrin beta1