Separation of porphyrin-based photosensitizer isomers by laser-induced fluorescence capillary electrophoresis.
Methods for the separation of photosensitizer isomers, such as benzoporphyrin derivative monoacid, benzoporphyrin ethyl monoacid, 2-[1-hexyloxyethyl]-2-devinylpyropheophorbide-a, diethyleneglycol diester benzoporphyrin derivative, tin ethyl etiopurpurin, and phthalocyanine tetrasulfonate, have been systematically developed by CE. Detection was accomplished by UV absorption at 214 nm or by LIF with excitation at 442/488 nm and emission at 690 nm. The effects of three major experimental parameters of buffer types, organic solvents, and surfactant additives are described. The optimized separation conditions were determined so as to provide satisfactory separation efficiency and analysis time. The methods are shown to be suitable for the separation and determination of porphyrin and phthalocyanines regioisomers, diastereoisomers, and enantiomers.
Peng
Xuejun
X
Department of Chemistry, University of British Columbia, Vancouver, BC, Canada.
Sternberg
Ethan
E
Dolphin
David
D
D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D007211:Indoles / Q000302:isolation & purification; D054833:Isoindoles; D017319:Photosensitizing Agents / Q000302:isolation & purification*; D011166:Porphyrins / Q000302:isolation & purification*; D012997:Solvents; D013237:Stereoisomerism; D013501:Surface-Active Agents
D002021:Buffers; D007211:Indoles; D054833:Isoindoles; D017319:Photosensitizing Agents; D011166:Porphyrins; D012997:Solvents; D013501:Surface-Active Agents; C013647:phthalocyanine