Effect of cycloheximide on in vitro development of electrically activated feline oocytes.
This study was conducted to improve parthenogenetic development in vitro of feline oocytes following a combined activation treatment of electrical stimulation and cycloheximide. In vitro matured (IVM) oocytes were stimulated electrically by a DC electrical pulse of 2 kV/cm for 50 micros. The stimulated oocytes were then incubated in MK-1 medium with or without cycloheximide and subsequently cultured in vitro for 6 days. No significant differences were observed between the two groups with respect to the proportions of cleavage, development to the morula stage, and the cell number of blastocysts. However, exposure of electrically stimulated oocytes to cycloheximide significantly increased the rate of development of the stimulated oocytes into the blastocyst stage compared with oocytes stimulated by electrical stimulation alone (31.0% vs 6.7%). The results from the present study suggested that a single electrical stimulation was insufficient to activate the IVM cat oocytes at 24 h of maturation and that exposure to cycloheximide following electrical stimulation improved the efficacy of the parthenogenetic development of domestic cat oocytes.
Karja
Ni Wayan Kurniani
NW
Laboratory of Animal Reproduction, Department of Veterinary Sciences, Yamaguchi University, Japan.
Otoi
Takeshige
T
Murakami
Masako
M
Wongsrikeao
Pimprapar
P
Budiyanto
Agung
A
Fahrudin
Mokhamad
M
Nagai
Takashi
T
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002415:Cats; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology*; D004558:Electric Stimulation; D005260:Female; D066298:In Vitro Techniques; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D010312:Parthenogenesis / Q000187:drug effects* / Q000502:physiology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*
D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide