title: A CARD-FISH protocol for the identification and enumeration of epiphytic bacteria on marine algae.
abstract: A CARD-FISH protocol was developed and applied to analyse surface-associated bacteria on the marine algae Ulva lactuca, Delisea pulchra, Corallina officinalis, Amphiroa anceps, Porphyra sp. and Sargassum linearifolium. The combination of Alexa(546)-labelled tyramide as the reporter molecule with SYBR Green II counterstain allowed for superior detection of the hybridised probe fluorescence against plant tissue from which pigment autofluorescence has been reduced.
authors.affiliation: Centre for Marine Biofouling and Bio-Innovation, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
authors.forename: Niina A
authors.initials: NA
authors.lastname: Tujula
authors.forename: Carola
authors.initials: C
authors.lastname: Holmström
authors.forename: Marc
authors.initials: M
authors.lastname: Mussmann
authors.forename: Rudolf
authors.initials: R
authors.lastname: Amann
authors.forename: Staffan
authors.initials: S
authors.lastname: Kjelleberg
authors.forename: Gregory R
authors.initials: GR
authors.lastname: Crocetti
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017753:Ecosystem; D056890:Eukaryota / Q000382:microbiology*; D017930:Genes, Reporter; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008386:Marine Biology; D015345:Oligonucleotide Probes; D009930:Organic Chemicals; D011806:Quinolinium Compounds; D012623:Seawater / Q000382:microbiology*
chemical_list: C481052:Alexa fluor 546; D015345:Oligonucleotide Probes; D009930:Organic Chemicals; D011806:Quinolinium Compounds; C098798:SYBR Green II