title: Intracerebral xenotransplantation of semipermeable membrane- encapsuled pancreatic islets.
abstract: OBJECTIVE
  To identify the decreasing effect of xenotransplantion in combination with privileged sites on rejection and death of biological semipermeable membrane-(BSM) encapsulated implanted islets.
  
  
  METHODS
  After the BSM experiment in vitro, BSM-encapsulated SD rat's islet-like cell clusters (ICCs) were xenotransplanted into normal dog's brain. Morphological changes were observed under light and transmission electron microscope. The islets and apoptosis of implanted B cells were identified by insulin-TUNEL double staining.
  
  
  RESULTS
  The BSM used in our study had a favorable permeability, some degree of rigidity, lighter foreign body reaction and toxicity. The grafts consisted of epithelioid cells and loose connective tissue. Severe infiltration of inflammatory cells was not observed. The implanted ICCs were identified 2 mo later and showed typical apoptosis.
  
  
  CONCLUSIONS
  BSM xenotransplantation in combination with the privileged site can inhibit the rejection of implanted heterogeneous ICCs, and death of implanted heterogeneous B cells is associated with apoptosis.
authors.affiliation: Neurosurgery Department of Yiwu Central Hospital, 3rd Faculty of Medical College, Zhejiang University, Zhejiang Province, China. wk555@sohu.com
authors.forename: Zhao-Liang
authors.initials: ZL
authors.lastname: Xin
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Ge
authors.forename: Xiao-Kang
authors.initials: XK
authors.lastname: Wu
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Jia
authors.forename: Han-Tao
authors.initials: HT
authors.lastname: Hu
mesh_terms: D000818:Animals; D001921:Brain; D004285:Dogs; D005549:Foreign-Body Reaction; D006084:Graft Rejection; D006085:Graft Survival; D007515:Islets of Langerhans / Q000166:cytology* / Q000276:immunology; D016381:Islets of Langerhans Transplantation / Q000276:immunology / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014183:Transplantation, Heterologous / Q000276:immunology / Q000379:methods*