title: Interplay between the cis-prenyltransferases and polyprenol reductase in the yeast Saccharomyces cerevisiae.
abstract: Dolichol formation is examined in three Saccharomyces cerevisiae strains with mutations in the ERG20 gene encoding farnesyl diphosphate synthase (mevalonic acid pathway) and/or the ERG9 gene encoding squalene synthase (sterol synthesis pathway) differing in the amount and chain length of the polyisoprenoids synthesized. Our results suggest that the activities of two yeast cis-prenyltransferases Rer2p and Srt1p and polyprenol reductase are not co-regulated and that reductase may be the rate-limiting enzyme in dolichol synthesis if the amount of polyisoprenoids synthesized exceeds a certain level. We demonstrate that reductase preferentially acts on typical polyprenols with 13-18 isoprene residues but can reduce much longer polyprenols with even 32 isoprene residues.
authors.affiliation: Institute of Biochemistry and Biophysics PAS, Pawinskiego 5a str, 02-106 Warsaw, Poland. babel@ibb.waw.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Szkopinska
authors.forename: Ewa
authors.initials: E
authors.lastname: Swiezewska
authors.forename: Joanna
authors.initials: J
authors.lastname: Rytka
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004122:Dimethylallyltranstransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004286:Dolichols / Q000096:biosynthesis; D015966:Gene Expression Regulation, Fungal; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014166:Transferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D004286:Dolichols; D029701:Saccharomyces cerevisiae Proteins; D010088:Oxidoreductases; C106822:polyprenol reductase; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C511732:Srt1 protein, S cerevisiae; C106821:cis-prenyl transferase; D004122:Dimethylallyltranstransferase; C485360:RER2 protein, S cerevisiae