Involvement of peroxiredoxin I in protecting cells from radiation-induced death.
Peroxiredoxin I (Prx-I), a key member of the peroxiredoxin family, reduces peroxides and equivalents through the thioredoxin system. Our previous work has shown that expression of Prx-I in mammalian cells increases following ionizing radiation (IR), indicating that Prx-I actively responds to IR-induced reactive oxygen species (ROS) and suggesting that Prx-I plays an important role in protecting cells from IR-induced death. To test this hypothesis, we suppressed the expression of Prx-I in SW480 cells by RNA interference. Our results show that IR induces the expression of Prx-I in SW480 cells in a dose- and time-dependent manner. The recombinant siRNA vector targeting Prx-I dramatically reduced the expression of Prx-I in SW480 cells. When Prx-I was knocked down in SW480 cells, the cells exhibited a decreased growth rate, a reduced antioxidant capability following IR and became more sensitive to IR-induced apoptosis. Together, our results demonstrate that Prx-I plays an important role in protecting cells from IR-induced cell death, which might be through scavenging IR-induced ROS in the cells.
Zhang
Bo
B
Combined Injury Institute of Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury.
Su
Yongping
Y
Ai
Guoping
G
Wang
Yan
Y
Wang
Tao
T
Wang
Fengchao
F
D017209:Apoptosis / Q000528:radiation effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D020868:Gene Silencing; D006360:Heat-Shock Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D010544:Peroxidases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D054464:Peroxiredoxins; D011829:Radiation Dosage
D006360:Heat-Shock Proteins; D009363:Neoplasm Proteins; D010544:Peroxidases; D054464:Peroxiredoxins