Immobilization of enzymes on a microchannel surface through cross-linking polymerization.
A novel and facile method for the preparation of an enzyme-immobilized microreactor has been developed in which enzymes are immobilized as an enzyme-polymer membrane formed on the inner wall of the microchannel by a cross-linking polymerization method; the resulting microreactor shows excellent reaction performance and stability against denaturating agents.
Honda
Takeshi
T
Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 807-1 Shuku, Tosu, Saga 841-0052, Japan.
Miyazaki
Masaya
M
Nakamura
Hiroyuki
H
Maeda
Hideaki
H
D015374:Biosensing Techniques / Q000379:methods*; D002918:Chymotrypsin / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004151:Dipeptides / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D013379:Substrate Specificity
D003432:Cross-Linking Reagents; D004151:Dipeptides; D004800:Enzymes, Immobilized; C007866:glutarylphenylalanine-4-nitroanilide; D011138:Polytetrafluoroethylene; D002918:Chymotrypsin