Effect of SNC19/ST14 gene overexpression on invasion of colorectal cancer cells.
OBJECTIVE
To study the effect of SNC19/ST14 gene overexpression on invasion in vitro of colorectal cancer cells.


METHODS
The adhesion of SNC19/ST14 gene-transfected cells to ECM was measured by MTT assay. The cell movement was evaluated by wound healing assay. Cell invasion and migration were determined by invasion assay in vitro.


RESULTS
SNC19/ST14 gene overexpression could enhance invasion of colorectal cancer cells in vitro significantly and influence early cell adherence to ECM, but could not change cell movement significantly.


CONCLUSIONS
SNC19/ST14 gene overexpression increases the local invasion of colorectal cancer cells in vitro.
Ding
Ke-Feng
KF
Surgery of Oncology, The Second Affiliated Hospital, School of Medicine, Zhejinag University, Hangzhou 310009, Zhejiang Province, China.
Sun
Li-Feng
LF
Ge
Wei-Ting
WT
Hu
Han-Guang
HG
Zhang
Su-Zhan
SZ
Zheng
Shu
S
D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism*
D012697:Serine Endopeptidases; C485596:ST14 protein, human