title: Polyethyleneimine/chitosan hexamer-mediated gene transfection into intestinal epithelial cell cultured in serum-containing medium.
abstract: In search of an efficient nonviral vector, polyethyleneimine (PEI)-based vectors were examined. In general, the transfection efficiency of nonviral vectors is suppressed by serum. Here we show that PEI based vectors, particularly, the chitosan hexamer-PEI vector, could perform efficient gene transfection into intestinal epithelial cells (IEC-6) in the presence of serum. The conjugation order of the two polymers with a plasmid (first, chitosan hexamer; second, PEI) was found to be an important factor in enhancing transfection efficiency.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Yukiteru
authors.initials: Y
authors.lastname: Ouji
authors.forename: Ayako
authors.initials: A
authors.lastname: Yoshida-Terakura
authors.forename: Yuka
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Isamu
authors.initials: I
authors.lastname: Maeda
authors.forename: Masaya
authors.initials: M
authors.lastname: Kawase
authors.forename: Eiji
authors.initials: E
authors.lastname: Yamato
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Miyazaki
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Yagi