title: Detection and cloning of the gene encoding a protein produced by nonpathogenic mutants of Fusarium oxysporum.
abstract: Treatment of Fusarium oxysporum with 5-azacytidine, a potent inhibitor of DNA methylation, induced nonpathogenic mutants. Analysis of the protein expression pattern by two-dimensional gel electrophoresis revealed a protein that is present in yeast-form cells of the mutants but absent in those of the wild-type strain. N-terminal amino acid analysis indicated that this protein is identical to a region of a polypeptide encoded by a cDNA clone, sti35, previously identified as a heat shock gene in F. oxysporum. A genomic clone for sti35 was isolated and sequence analysis revealed an intron and two heat shock elements upstream of sti35. The analysis also revealed the presence of a leader sequence composed of 27 amino acid residues, which shares a common amino acid composition with leader sequences of the proteins located in the mitochondrial matrix. Different expression patterns of sti35 in the mutants and wild-type strain were demonstrated.
authors.affiliation: Center for Gene Research, Ehime University, Matsuyama 790-8566, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Akiyama
authors.forename: P
authors.initials: P
authors.lastname: Thanonkeo
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: T
authors.initials: T
authors.lastname: Ohguchi
authors.forename: R
authors.initials: R
authors.lastname: Takata