Decreased antioxidant enzyme expression and increased oxidative damage in erosive lichen planus of the vulva.
The aim of this study was to investigate whether increased oxidative stress occurs in erosive lichen planus of the vulva. Skin biopsies from six patients with untreated, histologically confirmed erosive lichen planus of the vulva were examined immunohistochemically using antibodies against antioxidant enzymes. The protein-bound lipid peroxidation products malondialdehyde (MDA) and 4-hydroxynonenale (4-HNE) and the oxidative DNA damage marker 8-hydroxy-2'-deoxyguanosine (8-OHdG) were investigated. Protein carbonyls as markers of protein oxidation were visualised using the dinitrophenylhydrazone (DNPH) method. Normal vulval tissues from 12 subjects served as controls. In vulval lichen planus tissue the enzymatic antioxidant defence was found to be significantly decreased in the epidermal layers. Furthermore, a significant increase of lipid peroxidation products and oxidative DNA damage was found within the epidermis. Protein oxidation occurred predominantly in the papillary dermis. This is the first study to demonstrate a decreased antioxidant defence and increased oxidative damage to lipids, DNA and proteins in lichen planus. These oxidative modifications point to pathophysiological alterations mainly within the basal cell layers of the epidermis and at the dermoepidermal junction. Further studies are warranted to investigate the potential role of oxidative stress in the development of autoimmunity in this disease.
Sander
C S
CS
Dermatology Department, The Churchill Hospital, Oxford, UK.
Cooper
S M
SM
Ali
I
I
Dean
D
D
Thiele
J J
JJ
Wojnarowska
F
F
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000447:Aldehydes / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D004249:DNA Damage / Q000502:physiology; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D006801:Humans; D006834:Hydrazines / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008010:Lichen Planus / Q000201:enzymology*; D015227:Lipid Peroxidation / Q000502:physiology*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D012867:Skin / Q000201:enzymology; D014845:Vulvar Diseases / Q000201:enzymology*
D000447:Aldehydes; D000975:Antioxidants; D015415:Biomarkers; D006834:Hydrazines; C446799:dinitrophenylhydrazine; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; C027576:4-hydroxy-2-nonenal