title: Successful sperm storage for 28 years.
abstract: OBJECTIVE
  To report on two instances of successful long-term cryopreservation resulting in live births.
  
  
  METHODS
  Case report.
  
  
  METHODS
  The patient stored his sperm at a private sperm banking facility, and his partner underwent artificial insemination at an obstetrics/gynecology practice for one pregnancy and at a fertility center for the other.
  
  
  METHODS
  One man who stored his sperm before treatment for cancer.
  
  
  METHODS
  Storage of sperm under liquid nitrogen in a carousel canister system. Intrauterine insemination in which the semen was thawed, washed with human tubal fluid (HTF), and inseminated.
  
  
  METHODS
  Successful pregnancy resulting in live birth.
  
  
  RESULTS
  Artificial insemination with semen cryopreserved for 21 and 28 years resulted in two live births.
  
  
  CONCLUSIONS
  This case report describes the to-date longest known successful cryopreservation of sperm, with two live births resulting from IUI. Successful long-term semen storage can be very beneficial for men facing impaired fertility or sterility early in life, so that they have sufficient time to make appropriate family planning decisions.
authors.affiliation: Idant Laboratories, New York, New York, USA. jfeldschuh@daxor.com
authors.forename: Joseph
authors.initials: J
authors.lastname: Feldschuh
authors.forename: James
authors.initials: J
authors.lastname: Brassel
authors.forename: Nancy
authors.initials: N
authors.lastname: Durso
authors.forename: Allen
authors.initials: A
authors.lastname: Levine
mesh_terms: D000328:Adult; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006801:Humans; D050498:Live Birth; D008297:Male; D008875:Middle Aged; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*