title: Enhanced production of isoamyl alcohol and isoamyl acetate by ubiquitination-deficient Saccharomyces cerevisiae mutants.
abstract: Aromatic compounds are an important element in the flavor of yeast-fermented alcohol. We isolated mutants of Saccharomyces cerevisiae capable of growth at high levels of hydrostatic pressure. Among them, the HPG1 mutants, with a defect in their Rsp5 ubiquitin ligase, were found to produce high amounts of aromatics due to enhanced leucine uptake, with isoamyl alcohol production 2- to 3-fold and isoamyl acetate production 4- to 8-fold that of the wild-type strain. The result suggests that the HPG1/RSP5 mutant alleles could be new resources for producing these flavoring compounds for yeast-fermented alcoholic beverages.
authors.affiliation: Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. abef@jamstec.go.jp
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Abe
authors.forename: Koki
authors.initials: K
authors.lastname: Horikoshi
mesh_terms: D005800:Genes, Fungal; D006874:Hydrostatic Pressure; D007930:Leucine / Q000378:metabolism; D009154:Mutation; D000439:Pentanols / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D025801:Ubiquitin / Q000378:metabolism; D043743:Ubiquitin-Protein Ligase Complexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000439:Pentanols; D025801:Ubiquitin; C029683:isopentyl alcohol; D043743:Ubiquitin-Protein Ligase Complexes; D007930:Leucine; C020377:isoamyl acetate