Sulforaphane reduces infarct volume following focal cerebral ischemia in rodents.
Stroke is the third leading cause of death and disability in the United States. As several biochemical mechanisms have been proposed to contribute to stroke pathophysiology, treatments acting on multiple targets may be desirable. Sulforaphane (SUL), a naturally occurring isothiocyanate present in cruciferous vegetables, has been shown to induce the expression of multiple NF-E2-related factor-2 (Nrf2) responsive genes. In the present study, we demonstrate that systemically administered SUL can enter the brain as determined by increased mRNA and protein levels of the Nrf2-responsive gene heme oxygenase 1 (HO-1). Delayed administration (15 min) of a single dose of SUL significantly decreased cerebral infarct volume following focal ischemia, suggesting a potential therapeutic value for this compound.
Zhao
Jing
J
Vivian L. Smith Center for Neurologic Research, Department of Neurobiology and Anatomy, University of Texas Medical School, P.O. Box 20708, Houston, TX 77225, USA.
Kobori
Nobuhide
N
Aronowski
Jaroslaw
J
Dash
Pramod K
PK
D000704:Analysis of Variance; D000818:Animals; D015152:Blotting, Northern; D020520:Brain Infarction / Q000188:drug therapy* / Q000209:etiology; D002545:Brain Ischemia / Q000150:complications*; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D017879:Isothiocyanates; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013454:Sulfoxides; D013861:Thiocyanates / Q000737:chemistry / Q000627:therapeutic use*
D005904:Glial Fibrillary Acidic Protein; D017879:Isothiocyanates; D051267:NF-E2-Related Factor 2; D018696:Neuroprotective Agents; D012333:RNA, Messenger; D013454:Sulfoxides; D013861:Thiocyanates; D051547:Heme Oxygenase-1; D010751:Phosphopyruvate Hydratase; C016766:sulforaphane