title: Recurrent coamplification of cytoskeleton-associated genes EMS1 and SHANK2 with CCND1 in oral squamous cell carcinoma.
abstract: Chromosomal band 11q13 is frequently amplified in oral squamous cell carcinoma (OSCC) and assumed to be critically involved in tumor initiation and progression by proto-oncogene activation. Though cyclin D1 (CCND1) is supposed to be the most relevant oncogene, several additional putative candidate genes are inside this chromosomal region, for which their actual role in tumorigenesis still needs to be elucidated. To characterize the 11q13 amplicon in detail, 40 OSCCs were analyzed by comparative genomic hybridization to DNA microarrays (matrix-CGH) containing BAC clones derived from chromosomal band 11q13. This high-resolution approach revealed a consistent amplicon about 1.7 Mb in size including the CCND1 oncogene. Seven BAC clones covering FGF3, EMS1, and SHANK2 were shown to be frequently coamplified inside the CCND1 amplicon. Subsequent analysis of tissue microarrays by FISH revealed amplification frequencies of 36.8% (88/239) for CCND1, 34.3% (60/175) for FGF3, 37.4% (68/182) for EMS1, and 36.3% (61/168) for SHANK2. Finally, quantitative mRNA expression analysis demonstrated consistent overexpression of CCND1 in all tumors and of EMS1 and SHANK2 in a subset of specimens with 11q13 amplification, but no expression of FGF3 in any of the cases. Our study underlines the critical role of CCND1 in OSCC development and additionally points to the functionally related genes EMS1 and SHANK2, both encoding for cytoskeleton-associated proteins, which are frequently coamplified with CCND1 and therefore could cooperatively contribute to OSCC pathogenesis.
authors.affiliation: Abteilung Molekulare Genetik, B060, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: Kolja
authors.initials: K
authors.lastname: Freier
authors.forename: Carsten
authors.initials: C
authors.lastname: Sticht
authors.forename: Christof
authors.initials: C
authors.lastname: Hofele
authors.forename: Christa
authors.initials: C
authors.lastname: Flechtenmacher
authors.forename: Daniel
authors.initials: D
authors.lastname: Stange
authors.forename: Laura
authors.initials: L
authors.lastname: Puccio
authors.forename: Grischa
authors.initials: G
authors.lastname: Toedt
authors.forename: Bernhard
authors.initials: B
authors.lastname: Radlwimmer
authors.forename: Peter
authors.initials: P
authors.lastname: Lichter
authors.forename: Stefan
authors.initials: S
authors.lastname: Joos
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial; D051356:Cortactin / Q000235:genetics*; D056742:Cyclin D2; D016213:Cyclins / Q000235:genetics*; D003599:Cytoskeleton / Q000378:metabolism*; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009062:Mouth Neoplasms / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D000090063:Proto-Oncogene Mas
chemical_list: C080336:CCND2 protein, human; C495868:CTTN protein, human; D051356:Cortactin; D056742:Cyclin D2; D016213:Cyclins; C000715470:MAS1 protein, human; D009419:Nerve Tissue Proteins; D000090063:Proto-Oncogene Mas; C122162:SHANK2 protein, human