title: Comparison of the sperm aneuploidy rate in severe oligozoospermic and oligozoospermic men and its relation to intracytoplasmic sperm injection outcome.
abstract: OBJECTIVE
  To determine the incidence of disomy and diploidy for chromosomes 18, X, and Y in the sperm samples of severe oligozoospermic (<5 x 10(6) spermatozoa/mL) and oligozoospermic (5-20 x 10(6) spermatozoa/mL) men undergoing intracytoplasmic sperm injection (ICSI) and to evaluate the influence of sperm aneuploidy on pregnancy outcome.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Infertility clinic and genetic laboratory.
  
  
  METHODS
  Fifteen patients with severe oligozoospermia, 15 patients with oligozoospermia, and 10 normal fertile donors.
  
  
  METHODS
  Fluorescence in-situ hybridization (FISH) performed on sperm samples.
  
  
  METHODS
  The frequency of disomy and diploidy for chromosomes 18, X, and Y was analyzed using FISH, and the clinical outcome after ICSI was correlated.
  
  
  RESULTS
  Significantly greater frequencies of XY, YY disomy and diploidy were observed in severe oligozoospermic men compared with oligozoospermic and normozoospermic men. Although the fertilization rate was similar, the pregnancy rate was higher in the group with oligozoospermia versus severe oligozoospermia.
  
  
  CONCLUSIONS
  This study demonstrated the presence of an elevated sperm aneuploidy rate in patients with low semen quality. Additionally, the data show a negative influence of sperm chromosome abnormalities on ICSI outcome.
authors.affiliation: Jaslok Hospital and Research Centre, Mumbai, India.
authors.forename: Punam
authors.initials: P
authors.lastname: Nagvenkar
authors.forename: Kusum
authors.initials: K
authors.lastname: Zaveri
authors.forename: Indira
authors.initials: I
authors.lastname: Hinduja
mesh_terms: D000328:Adult; D000782:Aneuploidy; D016009:Chi-Square Distribution; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000706:statistics & numerical data; D008297:Male; D009845:Oligospermia / Q000453:epidemiology / Q000235:genetics*; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000453:epidemiology / Q000235:genetics*; D018873:Pregnancy Rate; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000706:statistics & numerical data*; D013094:Spermatozoa / Q000502:physiology*