title: Simultaneous speciation of selenium and sulfur species in selenized odorless garlic (Allium sativum L. Shiro) and shallot (Allium ascalonicum) by HPLC-inductively coupled plasma-(octopole reaction system)-mass spectrometry and electrospray ionization-tandem mass spectrometry.
abstract: The simultaneous speciation of selenium and sulfur in selenized odorless garlic (Allium sativum L. Shiro) and a weakly odorous Allium plant, shallot (Allium ascalonicum), was performed by means of a hyphenated technique, a HPLC coupled with an inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) equipped with an octopole reaction system (ORS). The aqueous extracts of them contained the common seleno compound that was identified as gamma-glutamylmethylselenocysteine by an electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Normal garlic contains alliin as the major sulfur-containing compound, which is the biological precursor of the garlic odorant, allicin. Alliin, however, was not detected in the extracts of the selenized odorless garlic. At least, four unidentified sulfur-containing compounds were detected in odorless garlic and shallot. Moreover, these Allium plants showed chemopreventive effects against human leukemia cells.
authors.affiliation: Department of Toxicology and Environmental Health, Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8675, Japan. ogra@p.chiba-u.ac.jp
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Ogra
authors.forename: Kazuya
authors.initials: K
authors.lastname: Ishiwata
authors.forename: Yuji
authors.initials: Y
authors.lastname: Iwashita
authors.forename: Kazuo T
authors.initials: KT
authors.lastname: Suzuki
mesh_terms: D000490:Allium / Q000737:chemistry*; D000603:Amino Acids, Sulfur / Q000032:analysis; D000972:Antineoplastic Agents, Phytogenic; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018922:HL-60 Cells; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012643:Selenium / Q000032:analysis*; D013455:Sulfur / Q000032:analysis*
chemical_list: D000603:Amino Acids, Sulfur; D000972:Antineoplastic Agents, Phytogenic; D013455:Sulfur; D012643:Selenium