title: Growth hormone induced lipolysis during short- and long-term administration in adult Prader-Willi patients.
abstract: Prader-Willi syndrome (PWS) is a complex genetic disease, clinically characterised by short stature, abnormal body composition, with more body fat than lean body mass, hyperphagia and obesity. Partial growth hormone (GH) deficiency is common, and GH treatment to PWS children and adults has shown beneficial effects on body composition. In this study, we have evaluated indices of GH's lipolytic effect in 6 PWS adults analysing glycerol, lactate and glucose in dialysate from microdialysis in subcutaneous abdominal adipose tissue. The patients were four men and two women, 19-37 years old; all hypogonadal. BMI was 24.2-49.1, mean 35.9 kg/m(2). All had normal serum insulin levels. They received GH therapy (Genotropin Pfizer) during 12 months and doses were individually titrated to normal serum IGF-I for age. Immediately before treatment start and at 12 months, 30-36 h after the last GH injection, sampling of dialysate was carried out at night (11 p.m. to 7 a.m.), as well as after intravenously injection of a standardised GH dose (0.8 mg). At baseline individual mean night time glycerol and lactate were similar to levels in adults without PWS (160.7-278.1 micromol/L and 0.80-3.99 mmol/L, respectively), and did not change with 12 months GH treatment. Glucose levels were normal, except in a patient with diabetes, and did not change during the study. Compared to baseline the immediate effect of GH injection resulted in a significant increase in glycerol levels after 12 months. In conclusion, night time lipolytic response in this small group of PWS adults seemed normal and did not change after 12 months GH treatment. On the other hand short-term GH induced lipolysis increased, indicating normal lipolytic response in PWS.
authors.affiliation: Department of Endocrinology, Metabolism and Diabetes, Karolinska University Hospital, Solna, 17176 Stockholm, Sweden. charlotte.hoybye@karolinska.se
authors.forename: Charlotte
authors.initials: C
authors.lastname: Höybye
authors.forename: Agneta
authors.initials: A
authors.lastname: Hilding
authors.forename: Claude
authors.initials: C
authors.lastname: Marcus
authors.forename: Marja
authors.initials: M
authors.lastname: Thorén
mesh_terms: D000273:Adipose Tissue; D000328:Adult; D001823:Body Composition; D015992:Body Mass Index; D002986:Clinical Trials as Topic; D005260:Female; D005838:Genotype; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism; D019382:Human Growth Hormone / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007006:Hypogonadism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008066:Lipolysis; D008297:Male; D017551:Microdialysis; D011218:Prader-Willi Syndrome / Q000378:metabolism*; D013997:Time Factors
chemical_list: D007773:Lactates; D019382:Human Growth Hormone; D019344:Lactic Acid; D007334:Insulin-Like Growth Factor I; D005947:Glucose; D005990:Glycerol