title: Immobilization of Arg-Gly-Asp (RGD) sequence in a thermosensitive hydrogel for cell delivery using pheochromocytoma cells (PC12).
abstract: A copolymer of N-isopropylacrylamide (98 mole% in the feed) and acrylic acid poly(N-isopropylacrylamide-co-acrylic acid) [p(NiPAAm-co-AAc)], and the adhesion molecule, an Arg-Gly-Asp (RGD)-incorporated hydrogel, were used to entrap pheochromocytoma cells (PC12). In a 28-d culture period, the PC12 cells in the RGD-conjugated gel maintained higher viability and produced dopamine at constant rates, while there was lower cell viability and less dopamine secretion by PC12 cells in p(NiPAAm-co-AAc). PC12 cells cultured in the RGD-conjugated gel would constitute a potentially useful three-dimensional cell system for application in nerve regeneration.
authors.affiliation: Department of Biomedical Engineering, Ajou University, Wonchon-dong Youngtong-gu, Suwon 442-749, Korea.
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
authors.forename: Kyusik
authors.initials: K
authors.lastname: Yun