Isolation and characterization of an alkaliphilic bacterium utilizing pyrene as a carbon source.
Alkaliphilic Mycobacterium sp. strain MHP-1, which can grow on pyrene as a sole carbon and energy source, was isolated from a soil sample. At the optimum pH for growth (pH 9), about 50% of pyrene (final concentration at 0.1% [w/v]) was degraded during 7 d of incubation, and 4,5-phenanthrenedioic acid, 4-phenanthroic acid and phthalic acid were identified as metabolic intermediates. Strain MHP-1 was found to possess aromatic-ring dioxygenase genes, which are highly homologous to the known nidAB genes from pyrene-degrading mycobacteria.
Habe
Hiroshi
H
Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. uhhabe@mail.ecc.u-tokyo.ac.jp
Kanemitsu
Mieko
M
Nomura
Mizuki
M
Takemura
Tetsuo
T
Iwata
Kenichi
K
Nojiri
Hideaki
H
Yamane
Hisakazu
H
Omori
Toshio
T