Effects of type II alveolar epithelial cells on T cell mitogenic responses to concanavalin A and purified protein derivatives.
To investigate the role of type II alveolar epithelial cells during the T cell-dependent host immune response to Mycobacterium tuberculosis (MTB), effects of MTB-infected A-549 human type II alveolar epithelial cells (A-549 cells) on T cell mitogenesis in response to concanavalin A (Con A) and purified protein derivatives (PPD) were studied. Human peripheral blood mononuclear cells (PBMCs) were cocultivated with uninfected or MTB-infected A-549 cells and Con A-and PPD-induced T cell mitogeneses were examined, and the following findings were obtained. T cell mitogenesis was inhibited by uninfected as well as MTB-infected A-549 cells, even when a dual-chamber culture system was used to prevent direct cell contact between PBMCs and A-549 cells. Therefore, it appears that A-549 cells suppress T cell mitogenesis by producing some unknown humoral suppressor factors.
Sato
Katsumasa
K
Department of Microbiology and Immunology, Shimane University School of Medicine, Izumo, Japan.
Shimizu
Toshiaki
T
Sano
Chiaki
C
Tomioka
Haruaki
H
D045744:Cell Line, Tumor; D018920:Coculture Techniques; D003208:Concanavalin A / Q000494:pharmacology*; D016207:Cytokines / Q000032:analysis; D004847:Epithelial Cells / Q000276:immunology*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology*
D016207:Cytokines; D015850:Interleukin-6; D008934:Mitogens; D014373:Tuberculin; D003208:Concanavalin A; D007213:Indomethacin