Effect of polymorphism of uncoupling protein 3 gene -55 (C>T) on the resting energy expenditure, total body fat and regional body fat in Chinese.
OBJECTIVE
To investigate the relationship of the C to T variant at the -55 site of the promoter region of uncoupling protein 3 gene (UCP3) with the resting energy expenditure and the parameters of body fat in Chinese population.


METHODS
Three hundred Chinese (91 normal weight subjects, 209 overweight/obesity subjects) were genotyped for the UCP3 gene -55(C>T) by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Resting energy expenditure (REE), fat mass (FM), fat free mass (FFM) and the parameters for regional adipose tissue distribution were measured.


RESULTS
Genotype frequencies of UCP3 gene -55(C>T) were not associated with obesity and different types of obesity. The REE level in normal weight subjects with TT homozygotes was higher than that in those with CT heterozygotes and CC homozygotes (P=0.0200). Similar tendency was also observed in overweight/obesity subjects. The FM/FFM exhibited significant difference between the overweight/obesity subjects with a TT genotype and those with a CT or CC genotype (P=0.0096).


CONCLUSIONS
The level of difference in REE caused by the polymorphism of promoter region of UCP3 -55(C>T) may play a role in energy metabolism in Chinese.
Fang
Qi-chen
QC
Shanghai Diabetes Institute, Department of Endocrinology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, PR China. fqichen@yahoo.com.cn
Jia
Wei-ping
WP
Yang
Ming
M
Bao
Yu-qian
YQ
Chen
Lei
L
Zhang
Rong
R
Xiang
Kun-san
KS
D000273:Adipose Tissue / Q000378:metabolism*; D044466:Asian People / Q000235:genetics; D002681:China; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000235:genetics* / Q000502:physiology; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D000071247:Uncoupling Protein 3
D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604392:UCP3 protein, human; D000071247:Uncoupling Protein 3