title: Identification of plasma membrane associated mature beta-hexosaminidase A, active towards GM2 ganglioside, in human fibroblasts.
abstract: Mature beta-hexosaminidase A has been found associated to the external leaflet of plasma membrane of cultured fibroblasts. The plasma membrane association of beta-hexosaminidase A has been directly determined by cell surface biotinylation followed by affinity chromatography purification of the biotinylated proteins, and by immunocytochemistry. The immunological and biochemical characterization of biotinylated beta-hexosaminidase A revealed that the plasma membrane associated enzyme is fully processed, suggesting its lysosomal origin.
authors.affiliation: Department of Medicina Sperimentale e Scienze Biochimiche, University of Perugia, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Mencarelli
authors.forename: Cristina
authors.initials: C
authors.lastname: Cavalieri
authors.forename: Alessandro
authors.initials: A
authors.lastname: Magini
authors.forename: Brunella
authors.initials: B
authors.lastname: Tancini
authors.forename: Luisa
authors.initials: L
authors.lastname: Basso
authors.forename: Peter
authors.initials: P
authors.lastname: Lemansky
authors.forename: Andrej
authors.initials: A
authors.lastname: Hasilik
authors.forename: Yu-Teh
authors.initials: YT
authors.lastname: Li
authors.forename: Vanna
authors.initials: V
authors.lastname: Chigorno
authors.forename: Aldo
authors.initials: A
authors.lastname: Orlacchio
authors.forename: Carla
authors.initials: C
authors.lastname: Emiliani
authors.forename: Sandro
authors.initials: S
authors.lastname: Sonnino
mesh_terms: D002462:Cell Membrane / Q000201:enzymology*; D005347:Fibroblasts / Q000201:enzymology; D005678:G(M2) Ganglioside / Q000378:metabolism*; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D001619:beta-N-Acetylhexosaminidases / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005678:G(M2) Ganglioside; D001619:beta-N-Acetylhexosaminidases