Chiral separation of halogenated amino acids by ligand-exchange capillary electrophoresis.
The chiral separation of halogenated amino acids by ligand-exchange CE is described. Halogenated amino acids attracted increasing interest in recent years because of their physiological activities. Different chiral selectors, as there are L-4-hydroxyproline, L-histidine, and N-alkyl derivatives of L-4-hydroxyproline in form of their copper(II) complexes, are compared for their chiral recognition ability for halogenated amino acids. The influence of various parameters, such as selector concentration, pH, organic modifier, and field strength, on the resolution was investigated. All halogenated amino acids investigated were baseline-separated under optimized conditions.
Koidl
Julia
J
Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, Karl-Franzens-University, Graz, Austria.
Hödl
Heike
H
Schmid
Martin G
MG
Pantcheva
Svetlana
S
Pajpanova
Tamara
T
Gübitz
Gerald
G
D000596:Amino Acids / Q000302:isolation & purification*; D003300:Copper; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D006219:Halogens; D006639:Histidine / Q000302:isolation & purification; D006909:Hydroxyproline / Q000302:isolation & purification; D008024:Ligands; D013237:Stereoisomerism
D000596:Amino Acids; D006219:Halogens; D008024:Ligands; D006639:Histidine; D003300:Copper; D006909:Hydroxyproline