title: The logical repertoire of ligand-binding proteins.
abstract: Proteins whose conformation can be altered by the equilibrium binding of a regulatory ligand are one of the main building blocks of signal-processing networks in cells. Typically, such proteins switch between an 'inactive' and an 'active' state, as the concentration of the regulator varies. We investigate the properties of proteins that can bind two different ligands and show that these proteins can individually act as logical elements: their 'output', quantified by their average level of activity, depends on the two 'inputs', the concentrations of both regulators. In the case where the two ligands can bind simultaneously, we show that all of the elementary logical functions can be implemented by appropriate tuning of the ligand-binding energies. If the ligands bind exclusively, the logical repertoire is more limited. When such proteins cluster together, cooperative interactions can greatly enhance the sharpness of the response. Protein clusters can therefore act as digital logical elements whose activity can be abruptly switched from fully inactive to fully active, as the concentrations of the regulators pass threshold values. We discuss a particular instance in which this type of protein logic appears to be used in signal transduction-the chemotaxis receptors of E. coli.
authors.affiliation: TCM Group, Cavendish Laboratory, Madingley Road, Cambridge CB3 0HE, UK. ig224@cam.ac.uk
authors.forename: Ian
authors.initials: I
authors.lastname: Graham
authors.forename: Thomas
authors.initials: T
authors.lastname: Duke
mesh_terms: D002633:Chemotaxis; D004926:Escherichia coli; D008024:Ligands; D008962:Models, Theoretical; D011485:Protein Binding; D011487:Protein Conformation; D020239:Receptor Cross-Talk / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*; D049490:Systems Biology
chemical_list: D008024:Ligands; D011956:Receptors, Cell Surface