title: [Effect of laser irradiation of diode laser on healing of surgical wounds in rats].
abstract: The aim of this work was to continue in previous study, which concerns biostimulation of skin wound healing evaluated after 24, 48, 120, 168 hours and so complete the chronological continuance of the process during the first seven days. Male, Sprague-Dawley rats (n=21) were used for the experiment. The rats were divided into 3 groups of 7 animals. In general anaesthesia (combination of xylazine, ketamine and tramadol) under aseptic condition two 3,5 cm long parallel skin incisions were performed on the left and right side of the rats spine and immediately sutured. The left wounds were daily stimulated with the diode laser (670 nm). The right wounds were not stimulated and served as control. The specimens of skin wounds were removed for histological evaluation 72, 96 and 144 hours after surgery. The biological specimens were stained with hematoxylin and eosin and histopathologically evaluated. In summary, in our histomorphological study of the influence of laser irradiation on primary wound healing evaluated after 72, 96 and 144 hours was concluded, that the healing of stimulated wounds was accelerated in comparison with controls. The histological evaluation showed earlier regress of inflammatory phase, faster finishing of reepithelization and acceleration in maturation phase. Presented experimental study completes the previous study and achieves the positive effect of biostimulation on all phases of skin wound healing in vivo.
authors.affiliation: Ustav lekárskej biofyziky, Lekárska fakulta Univerzity Pavla Jozefa Safarika v Kosiciach, Slovenská republika.
authors.forename: B
authors.initials: B
authors.lastname: Vidinský
authors.forename: P
authors.initials: P
authors.lastname: Gál
authors.forename: T
authors.initials: T
authors.lastname: Toporcer
authors.forename: M
authors.initials: M
authors.lastname: Balogácova
authors.forename: Z
authors.initials: Z
authors.lastname: Hutnanova
authors.forename: R
authors.initials: R
authors.lastname: Kilík
authors.forename: J
authors.initials: J
authors.lastname: Bober
authors.forename: J
authors.initials: J
authors.lastname: Sabo
authors.forename: F
authors.initials: F
authors.lastname: Longauer
mesh_terms: D000818:Animals; D062109:Dermatologic Surgical Procedures; D028022:Low-Level Light Therapy; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000528:radiation effects*