Quantitative transcriptional profiling of ATDC5 mouse progenitor cells during chondrogenesis.
During the differentiation of a mouse chondroprogenitor cell line, ATDC5, an analysis of the transcription cartilage-related genes was carried out using real-time RT-PCR in a semiquantitative fashion. A total number of 104 genes both previously linked to chondrogenesis and hitherto not associated with the development of cartilage were analyzed. Parametric statistics, and unsupervised hierarchical and K-medians clustering approaches were used to analyze the gene expression during the sequential processes of proliferation, condensation, differentiation, maturation, and hypertrophic conversion of ATDC5 cells. The obtained data provided a robust determination of expression patterns that make possible an accurate assessment of the molecular events along the chondrogenic differentiation pathway. In addition, time-course expression profiles were described for eight highly regulated genes that have not been associated with chondrogenesis as yet. These included Cryab, Rcor2, Hig1, Bnip3, Mst4, Calml4, Gng2, and Islr.
Chen
Li
L
Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, Fredrik Bajers Vej 3B, 9220 Aalborg, Denmark.
Fink
Trine
T
Zhang
Xiao-Yan
XY
Ebbesen
Peter
P
Zachar
Vladimir
V
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D020219:Chondrogenesis; D002999:Clone Cells; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005809:Genes, Regulator; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D014158:Transcription, Genetic
D007328:Insulin