A novel neutralizing monoclonal antibody against cell-binding polypeptide of ricin.
One strain of neutralizing monoclonal antibody (MAb) against cell-binding polypeptide of ricin, named 3E1, was generated efficiently. The antibody recognized the linearity epitope of RTB located in a toxin structure domain characterized by Western blotting. The safe period of mice for intraperitoneal injection of 100 microg of antibody was 20 min after intraperitoneal injection of 2 microg of Ricin (10 times LD50). The neutralizing MAb we obtained could be developed into an immunotherapeutic agent to counteract the use of ricin as a terrorist or biological warfare weapon. It might be useful, as well, for antibody-based prophylaxis.
Guo
Jian-Wei
JW
Department of Molecular Immunology, Institute of Basic Medical Sciences, Beijing, P.R. China., Clinical Laboratory, JinCheng Hospital, Lan Zhou, P.R. China.
Shen
Bei-Fen
BF
Feng
Jian-Nan
JN
Sun
Ying-Xun
YX
Yu
Ming
M
Hu
Mei-Ru
MR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D020128:Inhibitory Concentration 50; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests / Q000379:methods; D010455:Peptides / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000633:toxicity; D012276:Ricin / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000633:toxicity; D023382:Toxicity Tests, Acute
D000911:Antibodies, Monoclonal; D010455:Peptides; D012276:Ricin