Synthesis of unsymmetrical tweezer receptor libraries and identification of receptors for lys-D-ala-D-ala in aqueous solution.
Libraries of "unsymmetrical" tweezer receptors, featuring a guanidinium head group as a carboxylate binding site and two independently synthesized peptidic arms, have been prepared and screened to identify receptors for the N-Ac-Lys-D-Ala-D-Ala tripeptide sequence. The binding properties of one such receptor structure, with dye-labeled N-Ac-Lys-D-Ala-D-Ala, were investigated. These studies demonstrated that when attached to the solid-phase, the receptor binds dye-labeled N-Ac-Lys-D-Ala-D-Ala, in buffered aqueous media, with mM binding affinity.
Shepherd
Jon
J
School of Chemistry, University of Southampton, UK.
Gale
Tom
T
Jensen
Kim B
KB
Kilburn
Jeremy D
JD
D000595:Amino Acid Sequence; D020650:Combinatorial Chemistry Techniques; D009842:Oligopeptides / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014867:Water / Q000737:chemistry*
D009842:Oligopeptides; D014867:Water; C043367:lysyl-alanyl-alanine