title: [Research on differential display genes of tolerant-damage induced by trichloroethylene].
abstract: OBJECTIVE
  To observe the differentially expressed genes of the human normal liver cell (L-02) induced by low concentration of trichloroethylene (TCE).
  
  
  METHODS
  The dose-effect relation of TCE toxicity was analyzed by means of MTT. 5 micromol/L was chosen as low concentration while 40 micromol/L as high concentration. Then L-02 were treated with low concentration, high concentration, pretreated with low concentration then attacked with high concentration of TCE respectively. Fluorescence differential display polymerase chain reaction (Fluoro DD-PCR) was used to search differentially expressed genes of the different treatment groups of TCE. Results 51 differential expressed strap were found, 11 differential straps have been cloned and sequenced. 9 of them were known gene, while 2 of them were new genes.
  
  
  CONCLUSIONS
  The differential straps would be identified to provide scientific base for research deeply the mechanism of low concentration TCE inducing adaptive response of L-02.
authors.affiliation: Department of Health Toxicology, Sun Yat-sen Medical University, Guangzhou 510080, China.
authors.forename: Qin-Zhi
authors.initials: QZ
authors.lastname: Wei
authors.forename: Zhi-Xiong
authors.initials: ZX
authors.lastname: Zhuang
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Yuan
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Huang
authors.forename: Xiyi
authors.initials: X
authors.lastname: Li
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Geng
mesh_terms: D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D014241:Trichloroethylene / Q000633:toxicity*
chemical_list: D014241:Trichloroethylene