Comparison of proliferation and motile activity between human keratinocytes isolated from skin and oral mucosa.
OBJECTIVE
Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. For healing of skin wounds the skin keratinocytes can be replaced by oral mucosa epithelial cells grown in vitro. The presented experiments were carried out in order to compare the proliferation, morphology, and migration between human keratinocytes isolated from human skin and oral mucosa.


METHODS
Human epidermal and oral mucosa keratinocytes from primary culture were used in all experiments. Cell motility and shape were determined using computer-aided methods.


CONCLUSIONS
It was demonstrated that although both cell types exhibit the same typical epithelial morphology, oral mucosa keratinocytes locomote significantly faster than skin keratinocytes. They also differ in proliferation activity. Oral mucosa keratinocytes exhibited faster growth and different actin cytoskeleton organisation than skin keratinocytes under in vitro conditions. Autologous oral mucosa keratinocytes may be expanded in vitro and used for skin wound healing in vivo.
Drukała
Justyna
J
Laboratory of Cell and Tissue Engineering, Department of Cell Biology, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland. justyna@mol.uj.edu.pl
Zarzecka
Joanna
J
Gojniczek
Katarzyna
K
Waligórska
Agnieszka
A
Zapała
Jan
J
Korohoda
Włodzimierz
W
D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D009061:Mouth Mucosa / Q000166:cytology*; D012867:Skin / Q000166:cytology*; D014945:Wound Healing