[Design of software for fluorescence microscopy system to measure cytosolic free calcium in living cell].
OBJECTIVE
To design software based on the double-wavelength fluorescence microscopy to measure cytosolic free calcium ([Ca2+]i) in living cell.


METHODS
Through analyzing the work principles of monochromator, the function relation between output wavelength and voltage was obtained. Based on the analysis results as the arithmetic of the software, the application was developed in the integration development environment of VC ++ 6.0, by using the framework of Microsoft Foundation Class Library (MFC) and multithread technology.


RESULTS
The authors has realized the software and constructed a fluorescence microscopy system using the software, PC, monochromator, fluorescence microscope, MPT and data acquisition to measure the [Ca2+]i in single living beta cell.


CONCLUSIONS
Using this software, the [Ca2+]i in living cell can be detected. It has been proved to be an effective tool for the research in cellular biophysics.
Sun
Jing
J
Song
Shu-yan
SY
Qu
An-lian
AL
D055592:Biophysical Phenomena; D001703:Biophysics; D002118:Calcium / Q000032:analysis*; D003199:Computer Systems; D005069:Evaluation Studies as Topic; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D012984:Software
D002118:Calcium