title: Cloning and characterization of the dnaKJ operon in Acetobacter aceti.
abstract: The dnaKJ operon of Acetobacter aceti was cloned and sequenced. The profile of the gene configuration was similar to that of other alpha-proteobacteria. In the DnaK and DnaJ proteins of A. aceti, the characteristic domains/motifs reported in other organisms were well conserved. This operon was transcribed in response to a temperature shift and exposure to ethanol/acetic acid. The overexpression of this operon in A. aceti resulted in improved growth compared to the control strain at high temperature or in the presence of ethanol, suggesting a correlation to resistance against stressors present during fermentation, although the overexpression did not increase the resistance to acetic acid.
authors.affiliation: Department of Fermentation Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan. okamoto@nodai.ac.jp
authors.forename: Akiko
authors.initials: A
authors.lastname: Okamoto-Kainuma
authors.forename: Wang
authors.initials: W
authors.lastname: Yan
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fukaya
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Tukamoto
authors.forename: Morio
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Yukimichi
authors.initials: Y
authors.lastname: Koizumi