Suppressive effects of fruit-juice concentrate of Prunus mume Sieb. et Zucc. (Japanese apricot, Ume) on Helicobacter pylori-induced glandular stomach lesions in Mongolian gerbils.
Helicobacter pylori (Hp) infection is an important factor in human gastric disorders, including chronic active gastritis, peptic ulcers, intestinal metaplasia and cancer. Since epidemiologic studies overwhelmingly agree on a protective influence of fruits and vegetables in reducing the risk of gastric neoplasia and processed foods made from Prunus mume Sieb. et Zucc. (Japanese apricot or "Ume" in Japanese) are traditionally known for their miscellaneous medical effects, in the present study we investigated the efficacy of a fruit-juice concentrate of Japanese apricot (CJA) in the glandular stomach of Hp-infected Mongolian gerbils. Hp-inoculated gerbils were given CJA in their drinking water at concentrations of 1 and 3% for 10 weeks. The microscopic scores for gastritis and mucosal hyperplasia in the CJA groups were significantly lower than in the Hp-inoculated control group, with dose-dependence. Real-time PCR was performed to quantitate Hp by demonstrating urease A gene amount using gerbils glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene as an internal control. Average relative urease A gene dosage in the glandular stomach in the 1 and 3% CJA and Hp-inoculated control groups was 26.6 +/- 11.6% (average +/- SE), 30.3 +/- 10.5%, 100 +/- 40.9%, respectively, the fruit-juice concentrate causing significant lowering (P<0.01 and P<0.05, respectively, with 1 and 3%). These findings suggest that suppressive effects on gastric cancer development might also be expected as a result of decreased numbers of Hp and improvement of Hp-induced chronic active gastritis on administration of CJA.
Otsuka
Takafumi
T
Division of Oncological Pathology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chukusa, Nagoya 464-8681, Japan.
Tsukamoto
Tetsuya
T
Tanaka
Harunari
H
Inada
Kenichi
K
Utsunomiya
Hirotoshi
H
Mizoshita
Tsutomu
T
Kumagai
Toshiko
T
Katsuyama
Tsutomu
T
Miki
Kazumasa
K
Tatematsu
Masae
M
D000284:Administration, Oral; D000818:Animals; D001628:Beverages; D005756:Gastritis / Q000382:microbiology* / Q000517:prevention & control*; D005849:Gerbillinae; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000235:genetics / Q000378:metabolism; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori / Q000472:pathogenicity; D007249:Inflammation; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D027861:Prunus / Q000737:chemistry*; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000517:prevention & control*
D010936:Plant Extracts; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)