[Genetic polymorphism of Y-chromosome short tandem repeat in Elunchun ethnic group of China].
OBJECTIVE
To reveal the allelic frequencies and haplotype frequencies of ten Y-chromosome short tandem repeats(STR) loci (DYS392, DYS438, DYS439, DYS456, DYS459, DYS460,DYS461, DYS462, DYS389I and DYS389II systems) in an Elunchun population sample.


METHODS
PCR and polyacrylamidegel electrophoresis(PAGE) followed by silver staining were performed to analyze the blood samples from 102 unrelated Elunchun male individuals.


RESULTS
The allele diversity values for each locus ranged from 0.418(DYS461) to 0.727(DYS389 I). Gene diversities were higher than 0.5 except DYS462(0.479) and DYS461 (GATAA7.2)(0.418). Furthermore, 101 haplotypes from 10 Y-chromosome STRs loci were found in the sample and a high haplotype diversity 0.99 was determined.


CONCLUSIONS
The above findings suggest that the ten Y-chromosome STR loci are valuable Y-specific markers for establishing Y-STR database, understanding human origin, paternity testing and personal identification.
Feng
Xue
X
The Key Laboratory of National Ministry of Health for Forensic Science, Xi'an Jiaotong University, Xi'an, Shaanxi, PR China.
Xu
Ping
P
Li
Sheng-bin
SB
D002681:China; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic