title: Ultrasound-microbubble-induced neovascularization in mouse skeletal muscle.
abstract: Ultrasound-microbubble (US-MB) interactions stimulate neovascularization in rat gracilis muscle (GM). We examined microvascular remodeling (MVR) in GMs of C57BL/6 and balb/C mice following ultrasonic MB destruction. A range of MB dosages were administered IV, and exposed GMs received US. Muscles harvested 3, 7 and 14 d posttreatment were stained for vascular markers and assessed for changes in microvessel number, diameter and length. Muscles receiving a low MB dose (LMBD) and US showed significant increases in microvascular density after 3 d, returning to sham levels after one week. A MB dose producing maximum capillary disruptions was then established. This high MB dose (HMBD) facilitated significant MVR in C57BL/6 mice after one week. Balb/C GMs exhibited neovascularization 3 d, but not 7 or 14 d, following US-HMBD treatment. We conclude that HMBD in C57BL/6 mice induces a more sustained neovascularization response compared to balb/C or LMBD-treated C57BL/6 muscles; however, this response is still impermanent.
authors.affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Chappell
authors.forename: Alexander L
authors.initials: AL
authors.lastname: Klibanov
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Price
mesh_terms: D000818:Animals; D002196:Capillaries / Q000000981:diagnostic imaging*; D003287:Contrast Media / Q000494:pharmacology; D006614:Hindlimb; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D045423:Microbubbles; D018613:Microscopy, Confocal; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000000981:diagnostic imaging; D018919:Neovascularization, Physiologic / Q000502:physiology*; D013045:Species Specificity; D018084:Ultrasonography, Interventional / Q000379:methods*
chemical_list: D003287:Contrast Media