title: Formate dehydrogenase in rice plant: growth stimulation effect of formate in rice plant.
abstract: NAD-dependent formate dehydrogenase (FDH, EC 1.2.1.2.) plays a key role in the final step of the catabolism of methanol in methylotrophs. In certain plant species, this enzyme is a mitochondrial and NAD-dependent enzyme. In this study, the complete cDNA sequence of the rice (Oryza sativa cv Hinohikari) FDH gene, which contains an open reading frame (ORF) of 1128 bp, was determined. The sequence corresponds to a protein of 376 amino acids and shows strong homology to the NAD-dependent FDHs from other plants. Our experimental results showed that the expression of the rice cDNA in Escherichia coli induced FDH activity, indicating that the cDNA encodes a functional peptide of FDH. The application of formate to the roots of rice seedlings leads to growth increment both in terms of the length of the aerial part and the fresh weight. The FDH activity and the accumulation of FDH transcripts were strongly enhanced in the root portions of the formate-fed plants. This enhancement indicates that the transcriptional regulation which responds to formate functions in the rice plant, and that formate can act as an effective inducer for FDH activity.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shiraishi
authors.forename: E
authors.initials: E
authors.lastname: Fukusaki
authors.forename: A
authors.initials: A
authors.lastname: Kobayashi