title: High-performance liquid chromatography-tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root.
abstract: High-performance liquid chromatography-tandem mass spectrometry has been used to identify isoflavone aglycones and glycosides in kudzu root. Fourteen isoflavones were detected. Among these, six were identified by comparison with authentic standards. Tentative identifications of the other isoflavones are based on UV spectra, mass spectra of protonated and deprotonated molecules, and MS-MS data. Several are reported for the first time in kudzu root. The bioactivity and bioavailability of isoflavone aglycones are usually greater than those of their glycosides. To improve the bioavailability of kudzu root isoflavones, crude beta-glycosidases prepared from microbes were used to hydrolyze the isoflavone glycosides. Several MS modes are combined not only to identify the isoflavones in kudzu root, but also to describe the biotransformation of kudzu root isoflavone glycosides. It is also proved that crude beta-glycosidases have high selectivity toward the O-glycosides of isoflavones.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.forename: Xiaozhe
authors.initials: X
authors.lastname: Zhang
authors.forename: Jiping
authors.initials: J
authors.lastname: Chen
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
authors.forename: Antonius
authors.initials: A
authors.lastname: Kettrup
mesh_terms: D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007529:Isoflavones / Q000032:analysis* / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D018517:Plant Roots / Q000378:metabolism*; D029908:Pueraria / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D007529:Isoflavones