Determination of selenium and iodine in human thyroids.
This study focuses on the determination of selenium and iodine in human thyroids. The glands were digested using nitric acid in a microwave oven. Selenium was determined by inductively coupled plasma optical emission spectrometry (ICP-OES) using a new sample introduction system consisting of a reduction system coupled to a hydride generation nebulizer (DHGN). Iodine was determined by using the Sandell-Kolthoff procedure. The detections limits were 0.2 ng/mL and 0.3 ng/mL for the determination method of selenium and iodine, respectively. The amount of iodine in the whole gland was 3.44 +/- 1.11 microg/g. The lowest iodine level was 2.34 microg/g and the highest 5.21 microg/g. The lowest selenium concentration for a single sample was 505 +/- 51 ng/g and the highest 1495 +/- 204 ng/g depending on the fraction of the gland selected.
Murillo
Miguel
M
Analytical Chemistry Center, Chemistry School, Science Faculty, Central University of Venezuela, Zip Code 47102, Caracas 1041-A, Venezuela. mmurillo@strix.ciens.ucv.ve
Carrión
Nereida
N
Quintana
Mercedes
M
Sanabria
Georgia
G
Ríos
Manuel
M
Duarte
Leopoldo
L
Ablan
Franklin
F
D006801:Humans; D007455:Iodine / Q000032:analysis*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012643:Selenium / Q000032:analysis*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods; D013961:Thyroid Gland / Q000033:anatomy & histology / Q000737:chemistry*
D007455:Iodine; D012643:Selenium