Production of ribonucleotides by autolysis of Hansenula anomala grown on Korean ginseng steaming effluent.
Hansenula anomala KCCM 11473, which grew well in Korean ginseng steaming effluent, was selected for the production of 5'-ribonucleotides. This strain had high RNA content. Optimal autolysis conditions were established to produce 5'-ribonucleotides (13.9 approximately 28.5 mg/g of biomass) at 55 degrees C and pH 5.0 for 24 h. 5'-Phosphodiesterase and adenyl deaminase were not effective in increasing the yield of 5'-ribonucleotides, but the yield of IMP increased significantly only after the addition of 1.0% adenyl deaminase.
Jae-Ho
Kim
K
Department of Genetic Engineering and Bio-Medicinal Resources Research Center, Paichai University, Taejon 302-735, Korea.
Byung-Hoon
Lee
L
Jong-Soo
Lee
L