title: Purification of human chorionic gonadotropin from pregnancy urine by immunoaffinity chromatography using a monoclonal antibody anti-beta chain hCG.
abstract: Human chorionic gonadotropin (hCG) is found in pregnancy urine and in urine from some cancer patients. It is found in a variety of forms, whose concentrations have clinical importance. A highly active preparation of hCG from pregnancy urine has been obtained by a simple method of immunoaffinity chromatography using a monoclonal antibody (MAb) against beta-subunit hCG. The recuperation of hCG from urine extract by this procedure was 84.18%, with a purification factor of 19.78. The specificity activity of the obtained hCG was 9705.36 IU/mg. The preparation appeared homogeneous by SDS-PAGE, analytical gel filtration, and double immunodiffusion. The molecular mass was 40 kDa by gel filtration on Superose 12 HR. Molecular masses estimated by electrophoresis were 14.6 and 23.5 kDa for the alpha and beta subunits, respectively.
authors.affiliation: Laboratory of Protein Purification, Immunoassay Center, Cubanacán, Playa, Havana, Cuba. iqpurifica@ci,sld.cu
authors.forename: Antonio Melchor
authors.initials: AM
authors.lastname: Rodríguez
authors.forename: Orlando Zulueta
authors.initials: OZ
authors.lastname: Rodríguez
authors.forename: Ileana Baluja
authors.initials: IB
authors.lastname: Conde
authors.forename: Yanelis Robainas
authors.initials: YR
authors.lastname: Del Pino
authors.forename: Liliana Hernández
authors.initials: LH
authors.lastname: Pérez
authors.forename: Raquel López
authors.initials: RL
authors.lastname: Cisnero
authors.forename: Maritza Gutierrez
authors.initials: MG
authors.lastname: Duque
authors.forename: Julliette Cazanave
authors.initials: JC
authors.lastname: Mora
authors.forename: Dunia Bequer
authors.initials: DB
authors.lastname: Ariza
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D006063:Chorionic Gonadotropin / Q000302:isolation & purification* / Q000652:urine; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000276:immunology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D011247:Pregnancy
chemical_list: D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human