title: Preparation of donor lamellar tissue for deep lamellar endothelial keratoplasty using a microkeratome and artificial anterior chamber system: endothelial cell loss and predictability of lamellar thickness.
abstract: OBJECTIVE
  To measure endothelial cell loss and predictability of lamellar thickness after preparing donor tissue for deep keratoplasty with an artificial anterior chamber and microkeratome.
  
  
  METHODS
  A microkeratome set at a depth of 350 microm and a diameter of 9 mm was used to obtain ten lamellar lenticules from corneoscleral rims mounted in an artificial chamber. A punch trephine then was used to cut the donor tissue 7 mm in diameter. Specular microscopy was performed to evaluate endothelial cell density before the procedure, after cutting with the microkeratome, and after trephination. Pachymetry was performed to determine the predictability of lenticule thickness, before the procedure and after microkeratome incision.
  
  
  RESULTS
  Mean post-microkeratome endothelial cell loss was 79 +/- 88 cells/mm2 and post-punch trephination was 85 +/- 94 cells/mm2. This represented a mean percentage loss of 3.2% and 3.5% for the respective steps of this procedure. Nine of the ten lenticules were cut within +/- 75 microm of the intended 350-microm thickness.
  
  
  CONCLUSIONS
  Preparing donor lenticules for deep lamellar endothelial keratoplasty with a microkeratome with artificial chamber system caused a relatively small loss of endothelial cells (6.7% of the total) and a reproducible thickness. This may have advantages over manual preparation techniques.
authors.affiliation: John A Moran Eye Center, University of Utah, Salt Lake City, Utah, USA.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Kang
authors.forename: Marshall W
authors.initials: MW
authors.lastname: McEntire
authors.forename: Clinton J
authors.initials: CJ
authors.lastname: Thompson
authors.forename: Majid
authors.initials: M
authors.lastname: Moshirfar
mesh_terms: D000867:Anterior Chamber; D002452:Cell Count; D016039:Corneal Transplantation / Q000295:instrumentation / Q000379:methods*; D004728:Endothelium, Corneal / Q000473:pathology* / Q000637:transplantation*; D006801:Humans; D014019:Tissue Donors