title: Differential gene expression in HIV/SIV-associated and spontaneous lymphomas.
abstract: Diffuse large B-cell lymphoma (DLBCL) is more prevalent and more often fatal in HIV-infected patients and SIV-infected monkeys compared to immune-competent individuals. Molecular, biological, and immunological data indicate that virus-associated lymphomagenesis is similar in both infected hosts. To find genes specifically overexpressed in HIV/SIV-associated and non-HIV/SIV-associated DLBCL we compared gene expression profiles of HIV/SIV-related and non-HIV-related lymphomas using subtractive hybridization and Northern blot analysis. Our experimental approach allowed us to detect two genes (a-myb and pub) upregulated solely in HIV/SIV-associated DLBCLs potentially involved in virus-specific lymphomagenesis in human and monkey. Downregulation of the pub gene was observed in all non-HIV-associated lymphomas investigated. In addition, we have found genes upregulated in both non-HIV- and HIV-associated lymphomas. Among those were genes both with known (set, ND4, SMG-1) and unknown functions. In summary, we have demonstrated that simultaneous transcriptional upregulation of at least two genes (a-myb and pub) was specific for AIDS-associated lymphomas.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq. 2, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Nenasheva
authors.forename: A I
authors.initials: AI
authors.lastname: Nikolaev
authors.forename: A V
authors.initials: AV
authors.lastname: Martynenko
authors.forename: I B
authors.initials: IB
authors.lastname: Kaplanskaya
authors.forename: W
authors.initials: W
authors.lastname: Bodemer
authors.forename: G
authors.initials: G
authors.lastname: Hunsmann
authors.forename: V Z
authors.initials: VZ
authors.lastname: Tarantul