[Histological study of bone regeneration using vascular endothelial growth factor on rat mandibular bone defect].
OBJECTIVE
Local application of vascular endothelial growth factor (VEGF) was examined to determine whether it is effective in promoting bone regeneration.


METHODS
We operated on live 6-week male SD rat mandibular bone tissue to prepare bone defects in order to attempt the guided bone regeneration (GBR) method. Laboratory animals were divided into 3 groups: the control group, the fibrin group, and the VEGF group. Both radiological observation using soft x-ray and histological observation were performed respectively on the 10th, 20th and 30th days after the operations.


RESULTS
After 10 days, there was no apparent radio-photographic changes in the bone defects. However, after 20 days there were quite apparent changes, and after 30 days there were even more remarkable changes. The fibrin and VEGF groups changed more than the control group. In our histological observation, we observed rich capillary formation most notably in the VEGF group. This VEGF group also exhibited regeneration in the bone tissue.


CONCLUSIONS
Histological and soft x-ray radiological observations indicated that VEGF was effective for blood vessel formation, and VEGF offers potential for bone regeneration.
Yamashima
Takamori
T
Comprehensive Dental Care Unit, School of Dentistry at Niigata, The Nippon Dental University. yamasea@ngt.ndu.ac.jp
Yoshimura
Ken
K
Morita
Osami
O
Kobayashi
Kan
K
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D008297:Male; D008334:Mandible / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
D042461:Vascular Endothelial Growth Factor A