Neutralizing monoclonal antibodies against Russian strain of the West Nile virus.
We have developed a panel of 16 hybridomas secreting neutralizing monoclonal antibodies (Nt- MAbs) to Russian isolate (LEIV-Vlg99-27889-human) of the West Nile virus (WNV). Most of the Nt-Mabs were either IgG1 or IgG3 subtypes. Nine of the 16 neutralizing MAbs detected WNV protein E in Western blot. According to their Nt-activities, Western blot results and cross-reactivity, the MAbs were divided into four groups. Monoclonal antibodies from group I were able to neutralize WNV strains Vlg99-27889, Vlg00-27924, Hp-94, A-1640, A-72, Tur-2914, and Eg101. The Nt-activity of MAbs from groups II-IV towards these WNV strains was variable. Recombinant fragments E(1-180), E(1-321), and E(260-466) of protein E were used for preliminary mapping of domains recognized by Nt-MAbs. Only five Nt-MAbs were able to react with the recombinant polypeptides. The MAbs 9E2, 7G9, 11G3, and 7E6 from group Ia recognized Nt-epitope(s) between amino acids 321 and 466 of protein E and Nt-MAb 4F11 (group III) reacted with residues 1-180. This demonstrates that two discrete regions of protein E are involved in neutralization of WNV. Our data on immunochemical, biological activities of Nt-MAbs and mapping of Nt-epitopes using recombinant polypeptides suggest at least 13 different Nt-epitopes for WNV.
Razumov
Ivan A
IA
Institute of Molecular Biology, State Research Center of Virology and Biotechnology "Vector," Novosibirsk Region, Koltsovo 630559, Russia.
Kazachinskaia
Elena I
EI
Ternovoi
Vladimir A
VA
Protopopova
Elena V
EV
Galkina
Irina V
IV
Gromashevskii
Valentin L
VL
Prilipov
Alexander G
AG
Kachko
Alla V
AV
Ivanova
Alla V
AV
L'vov
Dimitry K
DK
Loktev
Valery B
VB
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics; D015153:Blotting, Western; D002522:Chlorocebus aethiops; D003429:Cross Reactions; D018604:Epitope Mapping; D006801:Humans; D006825:Hybridomas; D009500:Neutralization Tests; D012426:Russia; D014709:Vero Cells; D014902:West Nile virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral