title: Creating a Saccharomyces cerevisiae haploid strain having 21 chromosomes.
abstract: Chromosome engineering techniques that can manipulate a large segment of chromosomal DNA are useful not only for studying the organization of eukaryotic genomes but also for the improvement of industrially important strains. Toward the development of techniques that can efficiently manipulate a large segment of chromosome, we have previously reported a one-step chromosome splitting technique in a haploid Saccharomyces cerevisiae cell, with which we could successfully split yeast chromosome 11, XIII, or XI into two halves to create a haploid strain having 17 chromosomes. We have now constructed chromosome splitting vectors bearing ADE2, HIS3, LEU2, or TRP1 marker, and by using these vectors, we could successively split yeast chromosomes to create a novel yeast haploid strain having up to 21 chromosomes. The specific growth rates of yeast strains carrying more than 16 chromosomes up to 21 did not differ significantly, suggesting that yeast cells can harbor more chromosomes than they do in their natural state, that is, 16 chromosomes, without serious effects on their growth.
authors.affiliation: Department of Biotechnology, Graduate School ofEngineering, Osaka University, 2-1 Yamadaoka, Suita-shi, Osaka 565-0871, Japan.
authors.forename: Donny
authors.initials: D
authors.lastname: Widianto
authors.forename: Eishi
authors.initials: E
authors.lastname: Yamamoto
authors.forename: Minetaka
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Yukio
authors.initials: Y
authors.lastname: Mukai
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kaneko
authors.forename: Yasuji
authors.initials: Y
authors.lastname: Oshima
authors.forename: Masafumi
authors.initials: M
authors.lastname: Nishizawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Harashima