title: [Elucidation of the taxonomic status of industrial strains of thermophilic lactic acid bacteria by sequencing of 16S rRNA genes].
abstract: Phenotypic characteristics and results of PCR tests for the presence of species-specific genes indicate that a number of strains of thermophilic lactic acid bacteria previously considered as belonging to Streptococcus thermophilus are actually closely related to enterococci. In the present study, partial (over 500 nucleotides) sequencing of 16S rRNA genes from 12 strains of thermophilic lactic acid bacteria used as starters for manufacturing sour milk products on the territory of the Commonwealth of Independent States (CIS) has been performed. According to the results of the sequencing, seven of the strains have been classified with Enterococcus durans. The earlier classification (based on PCR tests) of two of the strains as S. thermophilus and three of the strains as E. faecium has been confirmed. The data obtained demonstrate that the enterococci E. durans and E. faecium are widely used as thermophilic starters for manufacturing sour milk products on the territory of the CIS.
authors.forename: S G
authors.initials: SG
authors.lastname: Botina
authors.forename: A M
authors.initials: AM
authors.lastname: Lysenko
authors.forename: V V
authors.initials: VV
authors.lastname: Sukhodelets
mesh_terms: D001483:Base Sequence; D043302:Cultured Milk Products / Q000382:microbiology*; D016983:Enterococcus / Q000145:classification* / Q000235:genetics; D007218:Industrial Microbiology; D008969:Molecular Sequence Data; D010641:Phenotype; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D048249:Streptococcus thermophilus / Q000145:classification* / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S