title: Cloning and heterologous expression of a glucodextranase gene from Arthrobacter globiformis I42, and experimental evidence for the catalytic diad of the recombinant enzyme.
abstract: The gene encoding a glucodextranase from Arthrobacter globiformis I42 was cloned and, subsequently, heterologously expressed in Escherichia coli. This glucodextranase gene consists of 1048 amino acid residues with a calculated molecular mass of 109,135 Da. The roles of two residues at the active site of A. globiformis I42 glucodextranase were examined by site-directed mutagenesis. Glutamic acid residues 458 and 656, which are part of the apparent catalytic residues, were found to be essential for hydrolase activity.
authors.affiliation: Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060-8589, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Morimoto
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Yasukawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Takehiro
authors.initials: T
authors.lastname: Unno
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ito
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsui