Constitutive expression of the SAP1 gene from willow (Salix discolor) causes early flowering in Arabidopsis thaliana.
SAP1-1 and SAP1-2 were isolated from the male reproductive buds of willow (Salix discolor, clone S365). SAP1-1 differs from SAP1-2 based on a few nucleotide substitutions, but the sizes of their full-length cDNAs are identical. The deduced amino acid sequences of SAP1-1 and SAP1-2 were 98% similar and contain the same C-terminal amino acid motif "GYGA" like that of PTAP1-2 from Populus trichocarpa. The expression patterns of SAP1 in various parts of the male reproductive buds of S. discolor implicate this gene in the formation of the inflorescence meristems, bracts, and floral meristems. To characterize the functions of SAP1, we assessed Arabidopsis thaliana transformed with 35S: :SAP1-1. A total of 52 transgenic T1 lines were obtained, and a 3:1 segregation ratio was obtained in the T2 generation of each line. In the T3 generation, five homozygous transgenic lines were obtained, which were used for further analysis. Screening of transgenic lines was greatly facilitated by the detection of GFP expression starting with germinating seeds. Phenotypes of the homozygous transgenic lines included early flowering, conversion of inflorescence branches to solitary flowers, formation of terminal flowers, and formation of flowers with greater number of petals, stamens, and pistils. Northern analysis showed similar expression levels in all five lines. This study provides the first functional analysis of an APETALA1 (AP1)/SQUAMOSA (SQUA) homolog from a dioecious species and suggests that SAP1 is a homolog of the AP1/SQUA gene.
Fernando
Danilo D
DD
Department of Environmental and Forest Biology, State University of New York College of Environmental Science and Forestry, 241 Illick Hall, 1 Forestry Drive, Syracuse, NY 13210, USA. fernando@esf.edu
Zhang
Shiliang
S
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D018076:DNA, Complementary / Q000302:isolation & purification; D035264:Flowers / Q000235:genetics / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D026161:MADS Domain Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D032108:Salix / Q000235:genetics*
C091535:AP1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D026161:MADS Domain Proteins