Development of a ligand-directed approach to study the pathogenesis of invasive aspergillosis.
Invasive aspergillosis is a leading cause of infectious death in immunosuppressed patients. Here, we adapted a phage display library-based selection to screen and identify binding peptides to the surface of Aspergillus fumigatus conidia and hyphae. We identified a peptide (sequence CGGRLGPFC) that reliably binds to the surface of Aspergillus fumigatus hyphae. Binding was not Aspergillus strain specific, as it was also observed in hyphae of other Aspergillus clinical isolates. Furthermore, CGGRLGPFC-displaying phage targets Aspergillus fumigatus hyphae on formalin-fixed paraffin-embedded histopathology sections of lung tissue recovered from mice with invasive pulmonary aspergillosis. This approach may yield reagents such as peptidomimetics for novel diagnostic and therapeutic interventions in invasive aspergillosis.
Lionakis
Michail S
MS
Department of Infectious Diseases, Infection Control, and Employee Health, Unit 402, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA.
Lahdenranta
Johanna
J
Sun
Jessica
J
Liu
Wei
W
Lewis
Russell E
RE
Albert
Nathaniel D
ND
Pasqualini
Renata
R
Arap
Wadih
W
Kontoyiannis
Dimitrios P
DP
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001228:Aspergillosis / Q000145:classification / Q000382:microbiology*; D001232:Aspergillus fumigatus / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity*; D001665:Binding Sites; D005260:Female; D025301:Hyphae / Q000378:metabolism; D008024:Ligands; D008168:Lung / Q000382:microbiology; D051379:Mice; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D015203:Reproducibility of Results
D008024:Ligands; D019151:Peptide Library; D010455:Peptides