title: Suppression of cell death in primary rat hepatocytes by alpha1-acid glycoprotein.
abstract: In screening for effective additives for the long-term culture of hepatocytes, the hepatoprotective effect of alpha1-acid glycoprotein (AGP) was observed. AGP prevented primary hepatocytes from undergoing cell death induced by the chemical toxin, bromobenzene. Moreover, AGP added to medium was found to maintain the number of viable hepatocytes for as long as 6 d. The hepatoprotective effect of AGP was lost by removing sialic acid groups at the N-glycan chain terminal of AGP. It is shown that the complete form of N-glycan chain is needed for the hepatoprotectivity of AGP.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Noritaka
authors.initials: N
authors.lastname: Kagaya
authors.forename: Akiko
authors.initials: A
authors.lastname: Kamiyoshi
authors.forename: Yoh-Ichi
authors.initials: Y
authors.lastname: Tagawa
authors.forename: Soichiro
authors.initials: S
authors.lastname: Akamatsu
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Isoda
authors.forename: Masaya
authors.initials: M
authors.lastname: Kawase
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Yagi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001798:Blood Proteins / Q000494:pharmacology*; D001969:Bromobenzenes / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology; D004347:Drug Interactions; D006023:Glycoproteins / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D009961:Orosomucoid; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001798:Blood Proteins; D001969:Bromobenzenes; D006023:Glycoproteins; C506108:Orm1 protein, rat; D009961:Orosomucoid; C032036:bromobenzene