A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex.
The mammalian Tip49a and Tip49b proteins belong to an evolutionarily conserved family of AAA+ ATPases. In Saccharomyces cerevisiae, orthologs of Tip49a and Tip49b, called Rvb1 and Rvb2, respectively, are subunits of two distinct ATP-dependent chromatin remodeling complexes, SWR1 and INO80. We recently demonstrated that the mammalian Tip49a and Tip49b proteins are integral subunits of a chromatin remodeling complex bearing striking similarities to the S. cerevisiae SWR1 complex (Cai, Y., Jin, J., Florens, L., Swanson, S. K., Kusch, T., Li, B., Workman, J. L., Washburn, M. P., Conaway, R. C., and Conaway, J. W. (2005) J. Biol. Chem. 280, 13665-13670). In this report, we identify a new mammalian Tip49a- and Tip49b-containing ATP-dependent chromatin remodeling complex, which includes orthologs of 8 of the 15 subunits of the S. cerevisiae INO80 chromatin remodeling complex as well as at least five additional subunits unique to the human INO80 (hINO80) complex. Finally, we demonstrate that, similar to the yeast INO80 complex, the hINO80 complex exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding.
Jin
Jingji
J
Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
Cai
Yong
Y
Yao
Tingting
T
Gottschalk
Aaron J
AJ
Florens
Laurence
L
Swanson
Selene K
SK
Gutiérrez
José L
JL
Coleman
Michael K
MK
Workman
Jerry L
JL
Mushegian
Arcady
A
Washburn
Michael P
MP
Conaway
Ronald C
RC
Conaway
Joan Weliky
JW
D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry; D002384:Catalysis; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry*; D042002:Chromatin Assembly and Disassembly; D002845:Chromatography; D002875:Chromosomes / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry; D004265:DNA Helicases / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism
D002352:Carrier Proteins; D002843:Chromatin; D018076:DNA, Complementary; D005656:Fungal Proteins; C483404:INO80 complex, S cerevisiae; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D004247:DNA; D000251:Adenosine Triphosphatases; C481261:Swr1 protein, S cerevisiae; D000074183:ATPases Associated with Diverse Cellular Activities; D004265:DNA Helicases; C486549:RUVBL1 protein, human; C122079:RUVBL2 protein, human