Centered reduced moments and associate density functions applied to alkaline comet assay.
The single cell gel electrophoresis assay is a sensitive, rapid, and visual technique for deoxyribonucleic acid (DNA) strand-break detection in individual mammalian cells, whose application has significantly increased in the past few years. The cells are embedded in agarose on glass slides followed by lyses of the cell membrane. Thereafter, damaged DNA strands are electrophoresed away from the nucleus towards the anode giving the appearance of a comet tail. Nowadays, charge coupled device cameras are attached at optical microscopes for recording the images of the cells, and digital image processing is applied for obtaining quantitative descriptors. However, the conventional software is usually expensive, inflexible and, in many cases, can only provide low-order descriptors based in image segmentation, determination of centers of mass, and Euclidean distances. Associated density functions and centered reduced moments offer an effective and flexible alternative for quantitative analysis of the comet cells. We will show how the position of the center of mass, the lengths and orientation of the main semiaxes, and the eccentricity of such images can be accurately determined by this method.
Castaneda
Roman
R
Universidad Nacional de Colombia Sede Medellín, Physics School, Faculty of Science, A.A. 3840, Medellín, Colombia. rcastane@perseus.unalmed.edu.co
Pelaez
Alejandro
A
Marquez
Maria-Elena
ME
Abad
Pablo
P
D000465:Algorithms; D020552:Comet Assay / Q000379:methods*; D003627:Data Interpretation, Statistical; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted