title: [The study on the STAT3 labeled by GFP affecting the migration of BEL-7402 cell line].
abstract: Signal transducers and activators of transcription (STATs) are key DNA-binding proteins in JAK/STATs signal pathway. Aberrant expression and activation of STAT3 have been identified in many kinds of tumors. We report here that constitutive activation of STAT3 was present in BEL-7402 cells. We constructed the fusing genes of STAT3 (wild type/mutant) and GFP to study the function of constitutively activated STAT3 in BEL-7402 cells. By measuring the migration of the cells labeled by GFP-STAT3(WT/CYF), we proved that overexpression of STAT3(WT) could augment the migration of BEL-7402 cells, while STAT3(CYF) could decrease the migration.
authors.affiliation: The Jiangsu Key Laboratory of Molecular Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210097, China.
authors.forename: Run Lin
authors.initials: RL
authors.lastname: Yang
authors.forename: Fei Yan
authors.initials: FY
authors.lastname: Pan
authors.forename: Jun
authors.initials: J
authors.lastname: Yuan
authors.forename: Shi Ying
authors.initials: SY
authors.lastname: Guo
authors.forename: Hong Xia
authors.initials: HX
authors.lastname: Zhang
authors.forename: Dong Hong
authors.initials: DH
authors.lastname: Zhao
authors.forename: Chao Jun
authors.initials: CJ
authors.lastname: Li
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D015850:Interleukin-6; D050796:STAT3 Transcription Factor; D049452:Green Fluorescent Proteins