Micropatterned avidin arrays on silicon substrates via photolithography, self-assembly and bioconjugation.
Processes for micropatterning protein arrays on inorganic substrates have gained attention in the development of biosensors and clinical diagnostics. This study demonstrates a chemically selective method based on photolithographic deposition of gold patterns with the subsequent attachment of functionalized alkanethiols via molecular self-assembly. Selective capping of carboxy groups on alkanethiols by N-hydroxysuccinimide esters allowed the deposition of methoxypoly(ethylene glycol) silane as a blocking agent on unpatterned regions. Carboxylates were used to couple a form of avidin to create a microarray of protein. This microarray was successfully probed with biotinylated quantum dots. In-process characterization methods included grazing-angle Fourier-transform IR spectroscopy, ellipsometry, contact-angle goniometry, atomic-force microscopy and fluorescence microscopy.
Vail
Timothy L
TL
Department of Chemistry and Biochemistry, P.O. Box 5698, Flagstaff, AZ 86011-5698, USA. Timothy.Vail@nau.edu
Cushing
Kevin W
KW
Ingram
Jani C
JC
St Omer
Ingrid
I
D001360:Avidin / Q000737:chemistry*; D019921:Biotinylation; D006046:Gold / Q000737:chemistry; D018625:Microscopy, Atomic Force; D011092:Polyethylene Glycols / Q000737:chemistry; D040081:Protein Array Analysis; D012825:Silicon / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013388:Succinimides / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D013388:Succinimides; D013438:Sulfhydryl Compounds; D001360:Avidin; D011092:Polyethylene Glycols; D006046:Gold; C001426:N-hydroxysuccinimide; D012825:Silicon