Development of a solid phase microextraction-gas chromatography method to determine N-hydroxymethyl-N-methylformamide and N-methylformamide in urine.
A headspace solid phase microextraction (SPME) method has been developed to determine metabolites of dimethylformamide, N-hydroxymethyl-N-methylformamide, and N-methylformamide (NMF) as NMF in urine by gas chromatography with nitrogen-phosphorus detector (GC-NPD). An SPME holder with a 65-microm PDMS/DVB fiber coating was used. Optimal desorption conditions were 250 degrees C for 1 min, adsorption at 80 degrees C for 15 min, and 3.00 mL of sample in the headspace vial. The method presented good resolution, repeatability, recovery, detection limit, ruggedness and response linearity.
Knupp
Vagner Fernandes
VF
Chemistry Department, ICEx, Federal University of Minas Gerais, Av. Antonio Carlos 6627, C.P. 702, 31270901 Belo Horizonte, M.G., Brazil.
Leite
Edna Maria Alvarez
EM
de Lourdes Cardeal
Zenilda
Z
D002849:Chromatography, Gas / Q000379:methods*; D004126:Dimethylformamide / Q000031:analogs & derivatives* / Q000032:analysis; D005559:Formamides / Q000032:analysis*; D006801:Humans; D015203:Reproducibility of Results; D014556:Urine / Q000737:chemistry*
D005559:Formamides; C042331:N-hydroxymethyl-N-methylformamide; D004126:Dimethylformamide; C002950:methylformamide