Translocation of metals from fly ash amended soil in the plant of Sesbania cannabina L. Ritz: effect on antioxidants.
The plants of Sesbania cannabina Ritz grown on different amendments of fly ash (FA), have shown a high accumulation of metals (Fe, Mn, Zn, Cu, Pb and Ni). The highest accumulation of Fe the and lowest level of Ni were recorded in these plants. The different amendments of fly ash with garden soil (GS) were extracted with DTPA and the levels of metals were found to be decreased with an increase in fly ash application ratio from 10% to 50% FA. The analysis of the results showed an increase in the level of malondialdehyde (MDA) content of the roots for all the exposure periods. The maximum increases of 136% (roots) and 120% (leaves) were observed in MDA content at 100% FA after 30 d of growth of the plant, compared to GS. The level of antioxidants was found to increase for all the exposure periods in the roots of the plants to combat metal stress. At 30 d, the maximum increase of 57% (ascorbic acid) and 78% (free proline) was observed in the roots of the plants grown on 100% and 10% FA, respectively, as compared to their respective GS. At 90 d, a maximum increase of 42% (cysteine) and 117% (NPSH) was recorded in the roots of the plants grown on 25% and 100% FA, respectively, as compared to their respective GS. In leaves, a significant increase in antioxidants i.e. cysteine, NPSH and free proline content was recorded after 30 d, whereas no such trend was observed for the rest of the exposure periods. The chlorophyll and carotenoid contents increased with an increase in the FA amendment ratio from 10% to 50% FA for all the exposure periods as compared to GS. In both roots and leaves, the level of protein content increased in all the amendments and 100% FA at 30 d as compared to GS. Thus, there is a balance in the level of MDA content and level of antioxidants in the plants at 90 d. In view of its tolerance, the plants may be used for phytoremediation of metals from fly ash contaminated sites and suitable species for plantation on fly ash land fills.
Sinha
Sarita
S
Ecotoxicology and Bioremediation, Environmental Sciences Division, National Botanical Research Institute, Lucknow 226 001, India. sinha_sarita@rediffmail.com
Gupta
Amit K
AK
D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D001673:Biodegradation, Environmental; D002244:Carbon; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D060729:Coal Ash; D003545:Cysteine / Q000378:metabolism; D005308:Fertilizers; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D019216:Metals, Heavy / Q000378:metabolism* / Q000633:toxicity; D052638:Particulate Matter; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000187:drug effects / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D049931:Sesbania / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism* / Q000633:toxicity; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D060729:Coal Ash; D005308:Fertilizers; D019216:Metals, Heavy; D052638:Particulate Matter; D012989:Soil Pollutants; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D002734:Chlorophyll; D002338:Carotenoids; D008315:Malondialdehyde; D002244:Carbon; D011392:Proline; D003545:Cysteine; D001205:Ascorbic Acid