Cell cycle analysis of Chinese hamster ovary cells stimulated by phosphatidic acid in serum-free culture.
When recombinant Chinese hamster ovary cells were treated with pertussis toxin or genistein, not only lysophosphatidic acid (LPA) but also phosphatidic acid (PA) failed to stimulate progression through the cell cycle in serum-free culture, suggesting that PA and LPA induce cell growth through the same signal transduction pathway. Cell cycle analysis also indicates that cell growth promoted by PA results in enhanced protein production.
Yamaji
Hideki
H
Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. yamaji@kobe-u.ac.jp
Sakai
Kentaro
K
Joho
Tsukasa
T
Izumoto
Eiji
E
Fukuda
Hideki
H