title: Spectral unmixing of flavin autofluorescence components in cardiac myocytes.
abstract: We applied linear unmixing approach to reveal individual components of intrinsic flavin fluorescence signal recorded in living cardiac cells by spectrally resolved confocal microscopy. Responses of whole-cell autofluorescence to modulators of cell metabolism and respiration were used as a tool of separation of its components; their spectral profiles, estimated by principal component analysis, correspond to free FAD and FAD bound to different enzymes of electron transport chain.
authors.affiliation: Department of Biophotonics, International Laser Centre, Ilkovicova 3, 81219 Bratislava, Slovakia. dusan@ilc.sk
authors.forename: D
authors.initials: D
authors.lastname: Chorvat
authors.forename: J
authors.initials: J
authors.lastname: Kirchnerova
authors.forename: M
authors.initials: M
authors.lastname: Cagalinec
authors.forename: J
authors.initials: J
authors.lastname: Smolka
authors.forename: A
authors.initials: A
authors.lastname: Mateasik
authors.forename: A
authors.initials: A
authors.lastname: Chorvatova
mesh_terms: D000465:Algorithms; D000818:Animals; D019069:Cell Respiration / Q000502:physiology; D002478:Cells, Cultured; D005415:Flavins / Q000378:metabolism*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008164:Luminescent Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005415:Flavins; D008164:Luminescent Proteins