Transgene expression systems in plant, a natural bioreactor.
Plants are important resources that have been providing us food from the earliest times. The rapid advances that have taken place in plant genetic engineering have made it possible to modify plants to increase food production and contribute to environmental purification. Transgenic plants are gaining increasing attention from the industry as a natural bioreactor for the production of industrial and chemical products. Useful expression systems based on promoters to optimize transgene expression in plant cells, hold the key to maximizing the potential of this concept of molecular-farming or industrial plants. This review, which is devoted to the use of plants for heterologous protein production, is divided into three parts. First, we introduce the nature of plant promoters and strategies for the isolation of novel promoters. In the second part, various promoters showing high-level constitutive, organ-specific, or inducible expression, are summarized as useful tools for realizing the efficient transcription of transgenes. Finally, problems in the expression of foreign gene in plant cells and future prospects in plant biotechnology are discussed.
Yoshida
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Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma-shi, Nara 630-0101, Japan.
Shinmyo
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