Temperature-induced expression of phb genes in Escherichia coli and the effect of temperature patterns on the production of poly-3-hydroxybutyrate.
The plasmid pNDTM2 was constructed to contain the p(R)-p(L) promoter and the phbC, A, and B genes which code for PHB synthase, beta-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34 degrees C during the initial 10 h of cultivation to promote cell growth, and then it should be increased to 40 degrees C for induction. Then the temperature should be lowered to 37 degrees C after 5 h to relieve the effect of the heat shock.
Shi
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Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan.
Kyuwa
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Takasu
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Shimizu
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