title: Functional analysis of tunicamycin-inducible gene A polypeptide from Aspergillus niger.
abstract: Tunicamycin-inducible gene A polypeptide (TIGA) is a member of the protein disulfide isomerase (PDI) family and is suggested to facilitate the folding of nascent polypeptides. The functional properties of TIGA were investigated here. TIGA acted as an isomerase, catalyzing the refolding of denatured and reduced ribonuclease A. TIGA also exhibited chaperone activity in the refolding of denatured prochymosin but not in the refolding of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), indicating that it had substrate specificity with respect to chaperone activity. Detailed study with a series of thioredoxin-motif (trx-motif) mutants revealed that the 2 trx-motifs of TIGA were not equal in activity. The N-terminal trx-motif was more active than the C-terminal trx-motif, and the first cysteine in each trx-motif was necessary for isomerase activity.
authors.affiliation: Institute ofMicrobiology, Chinese Academy of Sciences, Beijing 100080,China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Liang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Ma
authors.forename: Yuying
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001234:Aspergillus niger / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005800:Genes, Fungal; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D019704:Protein Disulfide-Isomerases / Q000235:genetics / Q000378:metabolism; D017510:Protein Folding; D014415:Tunicamycin / Q000494:pharmacology*
chemical_list: D005656:Fungal Proteins; D014415:Tunicamycin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D019704:Protein Disulfide-Isomerases