Field-amplified sample injection and in-capillary derivatization for sensitivity improvement of the electrophoretic determination of histamine.
The feasibility of the combination of field-amplified sample injection (FASI) and in-capillary derivatization was explored for improving sensitivity of histamine in capillary electrophoresis (CE). Naphthalene-2,3-dicarboxaldehyde (NDA) was used as derivatization reagent. The reagent and sample was introduced by tandem mode. The derivatization was accomplished by at-inlet mode with standing time of 1.5 min. The combination of FASI and in-capillary derivatization was successfully achieved with about 400-fold concentration sensitivity enhancement compared to pre-capillary derivatization at the same set-up. The detection limit of concentration for histamine reached 1.25 x 10(-11) M by CE and fluorescence detection with S/N = 3. Parameters affecting FASI and in-capillary derivatization process including sample matrix, buffer concentration and reagent injection amount, were investigated.
Zhang
Li-Yao
LY
Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, China.
Sun
Meng-Xiang
MX
D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D005240:Feasibility Studies; D006632:Histamine / Q000031:analogs & derivatives* / Q000032:analysis*; D009281:Naphthalenes / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012966:Sodium Cyanide / Q000737:chemistry
D002021:Buffers; D009281:Naphthalenes; C027717:2,3-naphthalenedicarboxaldehyde; D006632:Histamine; D012966:Sodium Cyanide