title: Production of an immunosuppressive polysaccharide, AZ9, in the culture of Klebsiella oxytoca strain TNM3.
abstract: A soil bacterium, Klebsiella oxytoca TNM3, was found to produce a polysaccharide named AZ9 with immunosuppressive activities. The culture of K. oxytoca in a jar fermentor resulted in an extreme increase in the viscosity of the culture broth (>10,000 mPa.s) due to accumulation of the polysaccharide with a high molecular weight. The molecular weight of AZ9 was successfully controlled by supplementing ethylenediamine tetraacetic acid (EDTA) and Fe2+ ion to the culture. Under optimized culture conditions, 39 g/l AZ9 with an average molecular weight of 200 kDa was produced, which was effective in suppressing IgE antibody production in mice.
authors.affiliation: Tayca Corporation, Taisho-ku, Osaka 551, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Sugihara
authors.forename: Y
authors.initials: Y
authors.lastname: Oiso
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: H
authors.initials: H
authors.lastname: Ohmori