title: Biochemical preparation of L-ribose and L-arabinose from ribitol: a new approach.
abstract: L-ribose and L-arabinose were prepared biochemically from ribitol via a two-step reaction, by which the complete oxidation of ribitol to L-ribulose (approximately 98%) was achieved by the reaction of washed cells of Acetobacter aceti IFO 3281. The produced L-ribulose was then used as a substrate for the production of L-ribose and L-arabinose. The isomerization of L-ribulose to L-ribose and L-arabinose was carried out using L-ribose isomerase (L-RI) of Acinetobacter sp. strain DL-28 and L-arabinose isomerase (L-AI) of Mycobacterium smegmatis, respectively. At equilibrium, the ratio of L-ribose: L-ribulose was 70:30 and that of L-arabinose: L-ribulose was 90: 10. After a simple purification treatment, both pentoses could be crystallized without the use of column chromatography. The crystals were confirmed as L-ribose and L-arabinose by High-performance liquid chromatography (HPLC), Infrared (IR), Nuclear magnetic resonance (NMR) and optical rotation measurements.
authors.affiliation: Department of Biochemistry and Food Sciences, Faculty of Agriculture, Kagawa University, Kagawa 761-0795, Japan.
authors.forename: Z
authors.initials: Z
authors.lastname: Ahmed
authors.forename: T
authors.initials: T
authors.lastname: Shimonishi
authors.forename: S H
authors.initials: SH
authors.lastname: Bhuiyan
authors.forename: M
authors.initials: M
authors.lastname: Utamura
authors.forename: G
authors.initials: G
authors.lastname: Takada
authors.forename: K
authors.initials: K
authors.lastname: Izumori