title: Characterization of N-acetylmuramidase M-1 of Streptomyces globisporus produced by Escherichia coli BL21(DE3)pLysS.
abstract: A gene of Streptomyces globisporus encoding N-acetylmuramidase M-1 was cloned into the pET26b vector and expressed in Escherichia coli BL21(DE3)pLysS. Maximal activity for the purified enzyme was observed at 55 degrees C with an optimal pH of 5.3, and N-bromosuccinimide strongly inhibited lytic activity even at a concentration of 0.01 mM. The enzyme showed N,O-diacetylmuramidase activity.
authors.affiliation: Enzyme Laboratory, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: H J
authors.initials: HJ
authors.lastname: Seo
authors.forename: T
authors.initials: T
authors.lastname: Shimonishi
authors.forename: K
authors.initials: K
authors.lastname: Ohmiya
authors.forename: K
authors.initials: K
authors.lastname: Hayashi