title: Characterization of pepsinogen C as a potential biomarker for gastric cancer using a histo-proteomic approach.
abstract: We analyzed 74 cryostat sections of central gastric tumor, tumor margin, and normal gastric epithelium using ProteinChip Arrays and SELDI-TOF MS. One peak was significantly down-regulated in tumor tissue (P = 1.43 x 10(-6)) and identified as pepsinogen C using MS/MS analysis and immunodepletion. This signal was further characterized by immunohistochemistry. This work demonstrates that differentially expressed signals can be identified and assessed using a proteomic approach comprising tissue-microdissection, protein profiling, and immunohistochemistry.
authors.affiliation: Core Unit Chip Application (CUCA), Institute of Human Genetics and Anthropology, Friedrich Schiller University, 07740 Jena, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Melle
authors.forename: Günther
authors.initials: G
authors.lastname: Ernst
authors.forename: Bettina
authors.initials: B
authors.lastname: Schimmel
authors.forename: Annett
authors.initials: A
authors.lastname: Bleul
authors.forename: Roland
authors.initials: R
authors.lastname: Kaufmann
authors.forename: Merten
authors.initials: M
authors.lastname: Hommann
authors.forename: Konrad K
authors.initials: KK
authors.lastname: Richter
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Daffner
authors.forename: Utz
authors.initials: U
authors.lastname: Settmacher
authors.forename: Uwe
authors.initials: U
authors.lastname: Claussen
authors.forename: Ferdinand
authors.initials: F
authors.lastname: von Eggeling
mesh_terms: D015415:Biomarkers / Q000737:chemistry*; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D004848:Epithelium / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007834:Lasers; D013058:Mass Spectrometry; D020115:Pepsinogen C / Q000737:chemistry*; D010449:Peptide Mapping; D040081:Protein Array Analysis; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013274:Stomach Neoplasms / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; D020543:Proteome; D020115:Pepsinogen C