[Role of the cell transportion from mother to baby in the mechanism of HBV intrauterine infection].
OBJECTIVE
To explore the route that the HBsAg positive pregnant women's cell entered fetal circulation, and to study the mechanism of HBV intrauterine transmission that the cell transported HBV to fetus and infected fetus.


METHODS
123 pregnant women and 123 newborns were collected from December, 2001 to October, 2003 in Taiyuan Infectious Hospital. The cell transportion from mother to baby were determined by allele-specific PCR (AS-PCR) and heminested PCR(hemi-nPCR).


RESULTS
Regarding GSTM1, ACE as maternal specific alleles: 42 mother-baby pairs were recruited from hemocyte specimens as informative cases, then examining 26 (61.90%) newborns' blood cell out of 42 informative cases had maternal cell DNA. There was significantly association between the cell transportion and HBV intrauterine infection (chi2 = 8.58, P < 0.01). There was significantly association between the cell transportion and HBV DNA positive in the newborns' PBMC (chi2 = 10.10, P < 0.01) .


CONCLUSIONS
One factor of HBV intrauterine infection is in the result of the cell transportion from mother to baby; PBMC carrying HBV probably passed through placent barrier into fetus and infected them.
Wei
Jun-Ni
JN
Deparment of Epidemiology, Shanxi Medical University, Taiyuan 030001, China.
Wang
Su-Ping
SP
Feng
Li-Ping
LP
Shuang
Jie-Yü
JY
D000328:Adult; D002465:Cell Movement / Q000502:physiology*; D004279:DNA, Viral / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006509:Hepatitis B / Q000635:transmission*; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D007963:Leukocytes, Mononuclear / Q000821:virology; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious; D055815:Young Adult
D004279:DNA, Viral