Mutation in IRO1 tightly linked with URA3 gene reduces virulence of Candida albicans.
Gene deletion in the pathogenic fungus Candida albicans has relied heavily on a URA3 cassette and a recipient delta ura3 strain CAI4. The IRO1 gene adjacent to URA3 was inadvertently deleted during construction of CAI4. We report here that a mutation in IRO1 reduces virulence of C. albicans.
Chibana
Hiroji
H
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan. chibana@faculty.chiba-u.jp
Uno
Jun
J
Cho
Tamaki
T
Mikami
Yuzuru
Y
D000818:Animals; D002176:Candida albicans / Q000254:growth & development / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology; D004195:Disease Models, Animal; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D017353:Gene Deletion; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D016019:Survival Analysis; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D014774:Virulence / Q000235:genetics*
D005656:Fungal Proteins; C430663:IRO1 protein, Candida albicans; D014157:Transcription Factors