title: Synthesis of unsymmetrical tweezer receptor libraries and identification of receptors for lys-D-ala-D-ala in aqueous solution.
abstract: Libraries of "unsymmetrical" tweezer receptors, featuring a guanidinium head group as a carboxylate binding site and two independently synthesized peptidic arms, have been prepared and screened to identify receptors for the N-Ac-Lys-D-Ala-D-Ala tripeptide sequence. The binding properties of one such receptor structure, with dye-labeled N-Ac-Lys-D-Ala-D-Ala, were investigated. These studies demonstrated that when attached to the solid-phase, the receptor binds dye-labeled N-Ac-Lys-D-Ala-D-Ala, in buffered aqueous media, with mM binding affinity.
authors.affiliation: School of Chemistry, University of Southampton, UK.
authors.forename: Jon
authors.initials: J
authors.lastname: Shepherd
authors.forename: Tom
authors.initials: T
authors.lastname: Gale
authors.forename: Kim B
authors.initials: KB
authors.lastname: Jensen
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: Kilburn
mesh_terms: D000595:Amino Acid Sequence; D020650:Combinatorial Chemistry Techniques; D009842:Oligopeptides / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014867:Water / Q000737:chemistry*
chemical_list: D009842:Oligopeptides; D014867:Water; C043367:lysyl-alanyl-alanine