Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen induces a strong bend on binding to terminal repeat DNA.
During latency, the Kaposi's sarcoma-associated herpesvirus genome is maintained as a circular episome, replicating in synchrony with host chromosomes. Replication requires the latency-associated nuclear antigen (LANA) and an origin of latent DNA replication located in the viral terminal repeats, consisting of two LANA binding sites (LBSs) and a GC-rich sequence. Here, we show that the recruitment of a LANA dimer to high-affinity site LBS-1 bends DNA by 57 degrees and towards the major groove. The cooccupancy of LBS-1 and lower-affinity LBS-2 induces a symmetrical bend of 110 degrees . By changing the origin architecture, LANA may help to assemble a specific nucleoprotein structure important for the initiation of DNA replication.
Wong
Lai-Yee
LY
Department of Microbiology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.
Wilson
Angus C
AC
D000956:Antigens, Viral / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019288:Herpesvirus 8, Human / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism*; D020079:Terminal Repeat Sequences; D014775:Virus Activation
D000956:Antigens, Viral; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C109540:latency-associated nuclear antigen