Enhancement of isoflavone synthase activity by co-expression of P450 reductase from rice.
Plant cytochrome P450s interact with a flavoprotein, NADPH-cytochrome P450 reductase (CPR), to transfer electrons from NADPH. The gene for rice P450 reductase (RCPR) was cloned and expressed in Saccaromyces cerevisiae, where the specific activity of the expressed RPCR was 0.91 U/mg protein. When isoflavone synthase gene (IFS) from red clover, used as a model system of plant cytochrome P450, was co-expressed with RCPR in yeast, the production of genistein from naringein increased about 4.3-fold, indicating that the RCPR efficiently interacts with cytochrome P450 to transfer electrons from NADPH.
Kim
Dae Hwan
DH
Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk University, 143-701, Seoul, Korea.
Kim
Bong Gyu
BG
Lee
Hyo Jung
HJ
Lim
Yoongho
Y
Hur
Hor Gil
HG
Ahn
Joong-Hoon
JH
D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D019833:Genistein / Q000378:metabolism; D029902:Medicago / Q000201:enzymology* / Q000235:genetics; D009251:NADPH-Ferrihemoprotein Reductase / Q000096:biosynthesis* / Q000235:genetics; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D010105:Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System; D019833:Genistein; D010105:Oxygenases; C048489:isoflavone synthase; D009251:NADPH-Ferrihemoprotein Reductase