Metabolic conversion of 24-methyl-Delta25-cholesterol to 24-methylcholesterol in higher plants.
Feeding of chemically synthesized [27-13C]codisterol ([27-13C]2), [27-13C]24-epicodisterol ([27-13C]3), [23,24-2H2]codisterol ([23,24-2H2]2), and [26,27-2H6]24-methyldesmosterol ([26,27-2H6]8) to Oryza sativa cell cultures, followed by MS and NMR analysis of the biosynthesized dihydrobrassicasterol (9)/campesterol (10), revealed that both (24R)- and (24S)-epimers of 24-methyl-Delta25-cholesterol (2/3) were converted to 9 and 10 via the common intermediate 24-methyldesmosterol (8).
Takahashi
Kyoko
K
Kyoritsu University of Pharmacy, Shibakoen, Tokyo 105-8512, Japan. takahashi-ky@kyoritsu-ph.ac.jp
Nasu
Kozue
K
Mashino
Tadahiko
T
Morisaki
Masuo
M
Hara
Noriyuki
N
Fujimoto
Yoshinori
Y
D002478:Cells, Cultured; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D012275:Oryza / Q000378:metabolism; D010840:Phytosterols / Q000096:biosynthesis* / Q000737:chemistry; D010944:Plants / Q000378:metabolism*
D010840:Phytosterols; C021273:campesterol; D002784:Cholesterol