title: Tachycardia increases NADPH oxidase activity and RyR2 S-glutathionylation in ventricular muscle.
abstract: We have shown previously that electrically induced tachycardia effectively produces myocardial preconditioning. Among other effects, tachycardia increases calcium release rates in microsomal fractions enriched in sarcoplasmic reticulum (SR) isolated from dog cardiac ventricular muscle. Here, we report that preconditioning tachycardia increased twofold the NADPH oxidase activity of isolated SR-enriched microsomal fractions, measured as NADPH-dependent generation of superoxide anion and hydrogen peroxide. Tachycardia also augmented the association of rac1 and the NADPH oxidase cytosolic subunit p47(phox) to the microsomal fraction, without modifying the content of the membrane integral subunit gp91(phox). Microsomes from control animals displayed endogenous S-glutathionylation of cardiac ryanodine receptors (RyR2); in microsomal fractions isolated after tachycardia RyR2 S-glutathionylation levels were 1.7-fold higher than in controls. Parallel in vitro experiments showed that NADPH produced a transient increase in calcium release rates and enhanced 1.6-fold RyR2 S-glutathionylation in control microsomes but had marginal or no effects on microsomes isolated after tachycardia. Catalase plus superoxide dismutase, and the NADPH oxidase inhibitors apocynin and diphenyleneiodonium prevented the in vitro stimulation of calcium release rates and RyR2 S-glutathionylation induced by NADPH, suggesting NADPH oxidase involvement. Conversely, addition of reducing agents to vesicles incubated with NADPH markedly inhibited calcium release and prevented RyR2 S-glutathionylation. We propose that tachycardia stimulates NADPH oxidase activity, which by enhancing RyR2 redox modifications such as S-glutathionylation, would contribute to sustain faster calcium release rates during conditions of increased cardiac activity. This response may be an important component of tachycardia-induced preconditioning.
authors.affiliation: Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Casilla 70005, Santiago 7, Chile.
authors.forename: Gina
authors.initials: G
authors.lastname: Sánchez
authors.forename: Zully
authors.initials: Z
authors.lastname: Pedrozo
authors.forename: Raúl J
authors.initials: RJ
authors.lastname: Domenech
authors.forename: Cecilia
authors.initials: C
authors.lastname: Hidalgo
authors.forename: Paulina
authors.initials: P
authors.lastname: Donoso
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D004285:Dogs; D004789:Enzyme Activation; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006352:Heart Ventricles / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D019255:NADPH Oxidases / Q000096:biosynthesis*; D011499:Protein Processing, Post-Translational; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D013610:Tachycardia / Q000378:metabolism*
chemical_list: D019837:Ryanodine Receptor Calcium Release Channel; D019255:NADPH Oxidases; D005978:Glutathione; D002118:Calcium