Schistosoma japonicum: a method for transformation by electroporation.
Despite of our knowledge of genetic make up of schistosomes, a number of genes have not been characterized largely due to lack of effective transformation protocols. Here we present electroporation as a strategy for effective introduction of plasmids DNA into schistosomula and adults. Using plasmids of pEGFP-C1 as an expression vector, we first verified that the CMV promoter could direct EGFP to express in primary culture cells from Schistosoma japonicum. Subsequently, the plasmids were introduced into schistosomula and adults by electroporation and EGFP expression was demonstrated using molecular and microscopical methods. Our findings indicate that electroporation is an effective method for transformation of S. japonicum.
Yuan
Xiao-Song
XS
Department of Parasitology and Key Laboratory of Genomic Research, Anhui Medical University, Hefei, Anhui 230032, PR China.
Shen
Ji-Long
JL
Wang
Xue-Long
XL
Wu
Xue-Song
XS
Liu
De-Pei
DP
Dong
Hui-Fen
HF
Jiang
Ming-Seng
MS
D000818:Animals; D015153:Blotting, Western; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D018274:Electroporation / Q000592:standards*; D015870:Gene Expression; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D010957:Plasmids; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000235:genetics*; D014162:Transfection; D014170:Transformation, Genetic
D018509:DNA, Helminth; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins