Proteomic identification of activin receptor-like kinase-1 as a differentially expressed protein during hyaloid vascular system regression.
The hyaloid vascular system (HVS) is a transient network of capillaries that nourishes the embryonic lens and the primary vitreous of the developing eye. We used proteomic analysis and immunohistochemical staining to identify activin receptor-like kinase-1 (ALK1), a type I receptor for transforming growth factor-beta1, during the HVS regression phase. In addition, we overexpressed ALK1 in corneas implanted with bFGF (basic fibroblast growth factor) pellets and observed that ALK1 overexpression resulted in inhibition of bFGF-induced corneal neovascularization in vivo. Our data suggest that ALK1 may play a role in HVS regression during ocular development.
Albè
Elena
E
Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
Escalona
Elizabeth
E
Rajagopal
Rama
R
Javier
Joel A
JA
Chang
Jin-Hong
JH
Azar
Dimitri T
DT
D030201:Activin Receptors, Type I / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D030301:Activin Receptors, Type II; D000818:Animals; D001158:Arteries / Q000196:embryology; D003315:Cornea / Q000098:blood supply / Q000196:embryology; D004247:DNA / Q000235:genetics; D042783:Endothelial Cells / Q000737:chemistry; D005123:Eye / Q000098:blood supply* / Q000196:embryology*; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D007908:Lens, Crystalline / Q000196:embryology / Q000378:metabolism; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000235:genetics; D040901:Proteomics; D017539:Tunica Intima / Q000166:cytology; D014822:Vitreous Body / Q000196:embryology / Q000378:metabolism
D005346:Fibroblast Growth Factors; D004247:DNA; D030201:Activin Receptors, Type I; D030301:Activin Receptors, Type II; C498931:Acvrl1 protein, mouse