Cell culture in a closed nano-space.
The minimum size of a closed nano-space in which cells can survive was determined using 4-nl nanowells. One or two cells could divide in the nanowell. Our results suggest that the cell division activity in the nano-space is determined by the conflict between intercellular effects and consumption of substrates.
Doi
Tomoyuki
T
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Matsunaga
Sachihiro
S
Maeno
Emi
E
Tsuchiya
Kensuke
K
Higashi
Tsunehito
T
Misawa
Shigeki
S
Uchiyama
Susumu
S
Ooi
Takeshi
T
Nakao
Masayuki
M
Fukui
Kiichi
K