title: Development of a ligand-directed approach to study the pathogenesis of invasive aspergillosis.
abstract: Invasive aspergillosis is a leading cause of infectious death in immunosuppressed patients. Here, we adapted a phage display library-based selection to screen and identify binding peptides to the surface of Aspergillus fumigatus conidia and hyphae. We identified a peptide (sequence CGGRLGPFC) that reliably binds to the surface of Aspergillus fumigatus hyphae. Binding was not Aspergillus strain specific, as it was also observed in hyphae of other Aspergillus clinical isolates. Furthermore, CGGRLGPFC-displaying phage targets Aspergillus fumigatus hyphae on formalin-fixed paraffin-embedded histopathology sections of lung tissue recovered from mice with invasive pulmonary aspergillosis. This approach may yield reagents such as peptidomimetics for novel diagnostic and therapeutic interventions in invasive aspergillosis.
authors.affiliation: Department of Infectious Diseases, Infection Control, and Employee Health, Unit 402, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA.
authors.forename: Michail S
authors.initials: MS
authors.lastname: Lionakis
authors.forename: Johanna
authors.initials: J
authors.lastname: Lahdenranta
authors.forename: Jessica
authors.initials: J
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Russell E
authors.initials: RE
authors.lastname: Lewis
authors.forename: Nathaniel D
authors.initials: ND
authors.lastname: Albert
authors.forename: Renata
authors.initials: R
authors.lastname: Pasqualini
authors.forename: Wadih
authors.initials: W
authors.lastname: Arap
authors.forename: Dimitrios P
authors.initials: DP
authors.lastname: Kontoyiannis
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001228:Aspergillosis / Q000145:classification / Q000382:microbiology*; D001232:Aspergillus fumigatus / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity*; D001665:Binding Sites; D005260:Female; D025301:Hyphae / Q000378:metabolism; D008024:Ligands; D008168:Lung / Q000382:microbiology; D051379:Mice; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D015203:Reproducibility of Results
chemical_list: D008024:Ligands; D019151:Peptide Library; D010455:Peptides