Expression patterns of the implantation-associated genes in the uterus during the estrous cycle in mice.
The mRNA expression patterns of EGF, HB-EGF, Amphiregulin, EGF receptor, IGF-1, CSF-1, IL-1 alpha, IL-1 beta, IL-1 receptor type 1, IL-1 receptor antagonist, LIF, COX-1, COX-2, Mucin-1, calcitonin, and rat USAG-1 mouse homologue, all of which are involved in the process of conceptus implantation to the endometrium, were examined during the estrous cycle by means of real-time quantitative PCR. COX-2, HB-EGF, LIF, Mucin-1, CSF-1, IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist were temporally regulated during the estrous cycle and highly expressed during the estrous stage. In the case of COX-1, EGF, IGF-1, and EGF receptor, the highest mRNA expression was during the diestrous stage. In contrast, the rat USAG-1 mouse homologue mRNA expression did not change during the estrous cycle. These results indicate that rat USAG-1 mouse homologue expression at implantation might be specifically regulated by embryonic factors rather than the maternal environment.
Lee
Dong-Soo
DS
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Yanagimoto Ueta
Yoshiko
Y
Xuan
Xuenan
X
Igarashi
Ikuo
I
Fujisaki
Kozo
K
Sugimoto
Chihiro
C
Toyoda
Yutaka
Y
Suzuki
Hiroshi
H
D000818:Animals; D051545:Cyclooxygenase 1 / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000235:genetics; D010064:Embryo Implantation / Q000235:genetics*; D004815:Epidermal Growth Factor / Q000235:genetics; D030762:Estrous Cycle / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D053605:Leukemia Inhibitory Factor; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D018396:Mucin-1 / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D017526:Receptor, IGF Type 1 / Q000235:genetics; D017472:Receptors, Interleukin-1 / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000235:genetics; D014599:Uterus / Q000502:physiology*
C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D018396:Mucin-1; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D016173:Macrophage Colony-Stimulating Factor; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D017526:Receptor, IGF Type 1