Determination of 12 pueraria components by high-performance liquid chromatography-mass spectrometry.
Puerariae radix, a commonly used Chinese herb drug derived from the dried root of legume plant, contains a series of isoflavones as its chief pharmacologically active constituents. Using 12 pueraria components as markers, an LC-UV-MS method requiring less than 60 min, was developed for estimating the quality of pueraria samples within 60 min. Extracts were analyzed using a Cosmosil 5C18-MS column, by gradient elution with an aqueous solution of acetic acid and methanol-ACN at a flow-rate of 1.0 mL/min. Peaks were detected at 254 nm and each peak was identified by LC/MS. The reproducibilities (RSD) of this proposed method, on the basis of peak-area ratios from six replicate injections, were 0.93-1.42% (intraday) and 1.05-1.63% (interday) at a detection limit of 0.12-0.78 microg/mL. Most of the markers used in this study can be classified, respectively, into three major categories, namely, isoflavones, O-glycosidic isoflavones, and C-glycosidic isoflavones. The structures of the compounds were determined from LC-MS fragmentation data and data from the literature.
Lin
Ching-Che
CC
Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan, P.R. China. chingchekimo@yahoo.com.tw
Wu
Chuan-I
CI
Sheu
Shuenn-Jyi
SJ
D019342:Acetic Acid; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry*; D015203:Reproducibility of Results; D014867:Water
D006027:Glycosides; D007529:Isoflavones; D014867:Water; D019342:Acetic Acid