title: Effect of additives on transesterification activity of Rhizopus chinensis lipase.
abstract: The transesterification activity of powder lipase prepared from the purified lipase of Rhizopus chinensis cells by freeze-drying was quite low compared with that of acetone-dried cells. Additives which could enhance the transesterification activity of the freeze-dried powder lipase were screened. The freeze-dried lipases prepared with certain fatty acid methylesters or certain types of surfactants exhibited high transesterification activity. It was shown that not only the solubility of the freeze-dried lipase in n-hexane but also the organic solvent-stability was enhanced when methyl stearate was added to the lipase solution at the freeze-drying step.
authors.affiliation: Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yasuda
authors.forename: T
authors.initials: T
authors.lastname: Kiguchi
authors.forename: H
authors.initials: H
authors.lastname: Kasahara
authors.forename: H
authors.initials: H
authors.lastname: Ogino
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa