title: Molecular analysis of multi-drug resistant Salmonella typhi from urban paediatric population of Bangladesh.
abstract: The prevalence and genetic basis of resistance of multi-drug resistant (MDR) S typhi strains from an urban paediatric population was determined. Blood cultures performed on 109 cases of suspected typhoid fever yielded 30(27.5%) S typhi isolates. Of these, 20(67%) S typhi isolates were resistant to the common antimicrobials used in Bangladesh, eg, chloramphenicol, ampicillin, cotrimoxazole, streptomycin and tetracycline, while 6(20%) isolates were resistant only to streptomycin. However, all the isolates were sensitive to fluquinolones and cephalosporins. Molecular analysis demonstrated that all MDR strains possessed a single large transferable 98 MDal plasmid. On conjugation, chloramphenicol, ampicillin and cotrimoxazole resistance was transferred from MDR strains to E coli K-12. Restriction endonuclease analysis of plasmid DNA showed similar digest profiles of all 5 selected donors and their transconjugants. This trend of increasing resistant strains of S typhi, especially by the transferable plasmid is of major public health concern.
authors.affiliation: Deptt. of Clinical Microbiology, National Institute of Cardiovascular Disease (NICVD), Dhaka.
authors.forename: K Z
authors.initials: KZ
authors.lastname: Mamun
authors.forename: S
authors.initials: S
authors.lastname: Tabassum
authors.forename: S M Z H
authors.initials: SM
authors.lastname: Ashna
authors.forename: C A
authors.initials: CA
authors.lastname: Hart
mesh_terms: D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001459:Bangladesh; D002648:Child; D002675:Child, Preschool; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D007223:Infant; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D012485:Salmonella typhi / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D014505:Urban Population
chemical_list: D000900:Anti-Bacterial Agents