title: Mitochondrial dysfunction enhances Gal4-dependent transcription.
abstract: Mitochondrial dysfunction has been shown to elicit broad effects on nuclear gene expression. We show here that transcription dependent on the prototypical acidic activator Gal4 is responsive to mitochondrial dysfunction. In cells with no mitochondrial DNA, Gal4-dependent gene expression is elevated. A minimal Gal4 activator containing the DNA binding and activation domain is sufficient for this response. Transcription dependent on a fusion of Gal4 to a heterologous DNA binding domain is similarly elevated in a mitochondrial mutant. Analysis of different Gal4-dependent promoters and gel mobility shift assays suggest that the effect of mitochondrial dysfunction on Gal4 activity is related to increased DNA binding to the cognate Gal4 element. Given that fermentation is the only means to obtain energy in respiratory deficient cells, it is possible that higher Gal4 activity in cells with dysfunctional mitochondria works to promote more efficient fermentation of galactose.
authors.affiliation: Department of Molecular Biology, Ruder Bosković Institute, Bijenicka 54, 10000 Zagreb, Croatia.
authors.forename: Branka
authors.initials: B
authors.lastname: Jelicić
authors.forename: Ana
authors.initials: A
authors.lastname: Traven
authors.forename: Vedrana
authors.initials: V
authors.lastname: Filić
authors.forename: Mary
authors.initials: M
authors.lastname: Sopta
mesh_terms: D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins; D005285:Fermentation; D005690:Galactose / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005690:Galactose