Enhanced accumulation of anthocyanin in cultured strawberry cells by repetitive feeding of L-phenylalanine into the medium.
L-Phenylalanine (Phe), biosynthetic precursor of anthocyanin, was repetitively added into a suspension culture of strawberry cells, constantly producing anthocyanins. In the repetitive feeding culture, the maximum anthocyanin accumulation per culture was 30% and 81% higher than those in a single Phe-feeding culture and non-feeding culture, respectively. This means that the inhibitory effect of Phe on cell growth was successfully avoided and its intracellular free-state concentration was maintained at a higher level by the repetitive feeding of L-phenylalanine at relatively lower intra-medium concentration. These results support the phenylalanine pool hypothesis which states that the intracellular L-phenylalanine level determines anthocyanin production. However, it was also suggested that there exist other restrictions controlling intracellular anthocyanin accumulation.
Edahiro
Jun-Ichi
J
Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Nakamura
Masayuki
M
Seki
Minoru
M
Furusaki
Shintaro
S
D000872:Anthocyanins / Q000096:biosynthesis*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D031985:Fragaria / Q000187:drug effects* / Q000378:metabolism*; D010649:Phenylalanine / Q000008:administration & dosage*
D000872:Anthocyanins; D003470:Culture Media; D010649:Phenylalanine