[Cloning and bioinformatic analysis of a novel gene HCR2 up-regulated in aorta of hypercoagulable rat].
OBJECTIVE
The aim of this study was to clone the full-length cDNA of HCR2 up-regulated in aorta of hypercoagulable rat and make the relevant bioinformatic analysis.


METHODS
Rapid amplification of cDNA end (RACE) and nested PCR technique was used to amplify the full-length cDNA of HCR2 from an EST (GenBank accession number BQ901227) which was significantly up-regulated in hypercoagulable rat.


RESULTS
The full-length cDNA of HCR2 has been obtained (GenBank accession number AY234417). Semi-quantitative RT-PCR demonstrated that HCR2 is up-regulated in aorta of hypercoagulable rat. Bioinformatic analysis showed that HCR2 gene is located in rat chromosome 4q11. The full-length cDNA of HCR2 is 1275 bp, coding for a 78 amino acids polypeptide with a theoretical molecular weight of 8841.7 and isoelectric point of 8. 59. This protein has no signal peptide and transmembrane sequence. It may be a nuclear protein. It shares no significant homology with any known protein.


CONCLUSIONS
The successful cloning of HCR2 has laid a solid foundation for further study of its function and its possible role in the occurrence and development of hypercoagulability.
Zhao
Yu-Hua
YH
Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Liu
Bing-Wen
BW
Bai
Huai
H
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D051381:Rats; D019851:Thrombophilia / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
D018076:DNA, Complementary; D006023:Glycoproteins; C503567:LOC100364673