Rat epidermal keratinocyte organotypic culture (ROC) as a model for chemically induced skin irritation testing.
The potential of rat epidermal keratinocyte (REK) organotypic culture (ROC) with proper stratum corneum barrier as a model for screening skin irritants was evaluated. The test chemicals were selected from ECETOC database (1995) and the observed in vitro irritation potential was compared to ECETOC in vivo primary irritation index (PII), to EU risk phrases, and to the harmonized OECD criteria. Chemicals were applied onto the stratum corneum surface of ROC for 30 min and samples were taken from the underlying medium at 4 and 8 h after exposure. Cell membrane integrity (determined by LDH assay) and pro-inflammatory effect (determined by IL-1alpha release) were verified at both time points and correlated to PII values. The best correlation (R(2) = 0.831) was seen with LDH leakage test. Based on obtained data, chemicals were classified according to criteria defined by EU and OECD. From 12 chemicals, only two were incorrectly classified according to OECD criteria when using LDH leakage and IL-1alpha release as irritation markers. At the end of experiment, chemical-treated ROC cultures were fixed and histological changes were assessed. Typical signs for irritation were lightly stained cytoplasm, condensed nuclei, cellular vacuolization, eosinophilic cytoplasms, and blebbing. These irritation effects of chemicals were graded visually into four classes (A-D). The extent of morphological perturbations of the cultures mostly correlated with PII. The present results indicate the validity of the ROC model in predicting skin irritation potential of chemicals and show that the use of set of irritation markers with different mechanistic responses gives more information on irritation than if only one marker was used.
Pappinen
Sari
S
Department of Pharmaceutics, University of Kuopio, PO Box 1627, 70211 Kuopio, Finland. sari.pappinen@uku.fi
Pasonen-Seppänen
Sanna
S
Suhonen
Marjukka
M
Tammi
Raija
R
Urtti
Arto
A
D000818:Animals; D017209:Apoptosis; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006416:Hematoxylin / Q000737:chemistry; D007375:Interleukin-1 / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D008954:Models, Biological; D009930:Organic Chemicals / Q000008:administration & dosage / Q000009:adverse effects / Q000145:classification; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000503:physiopathology / Q000648:ultrastructure; D023422:Skin Irritancy Tests / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
D007375:Interleukin-1; D009930:Organic Chemicals; D007770:L-Lactate Dehydrogenase; D006416:Hematoxylin