Metalloproteomics: high-throughput structural and functional annotation of proteins in structural genomics.
A high-throughput method for measuring transition metal content based on quantitation of X-ray fluorescence signals was used to analyze 654 proteins selected as targets by the New York Structural GenomiX Research Consortium. Over 10% showed the presence of transition metal atoms in stoichiometric amounts; these totals as well as the abundance distribution are similar to those of the Protein Data Bank. Bioinformatics analysis of the identified metalloproteins in most cases supported the metalloprotein annotation; identification of the conserved metal binding motif was also shown to be useful in verifying structural models of the proteins. Metalloproteomics provides a rapid structural and functional annotation for these sequences and is shown to be approximately 95% accurate in predicting the presence or absence of stoichiometric metal content. The project's goal is to assay at least 1 member from each Pfam family; approximately 500 Pfam families have been characterized with respect to transition metal content so far.
Shi
Wuxian
W
New York Structural GenomiX Research Consortium, Bronx, New York 10461, USA.
Zhan
Chenyang
C
Ignatov
Alexander
A
Manjasetty
Babu A
BA
Marinkovic
Nebojsa
N
Sullivan
Michael
M
Huang
Raymond
R
Chance
Mark R
MR
D001665:Binding Sites; D019295:Computational Biology; D018360:Crystallography, X-Ray; D030562:Databases, Protein; D023281:Genomics; D008667:Metalloproteins / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D011485:Protein Binding; D040901:Proteomics; D020539:Sequence Analysis, Protein; D013052:Spectrometry, X-Ray Emission; D040681:Structural Homology, Protein; D013329:Structure-Activity Relationship
D008667:Metalloproteins