Utilization of swine wastewater as a feedstock for the production of polyhydroxyalkanoates by Azotobacter vinelandii UWD.
Azotobacter vinelandii UWD produced 0.69 g.l(-1) poly(hydroxybutyrate-co-hydroxyvalerate, PHBV) with 7.9 mol% hydroxyvalerate (HV) from twofold-diluted swine wastewater (SW). When supplemented with 20 g.l(-1) glucose, twofold-diluted SW increased copolymer production by 8.6 times. When three organic acids (acetate, propionate and butyrate) present in SW were supplemented with 20 g.l(-1) glucose, PHBV production was comparable (5.5 g.l(-1)) to that in the case of using twofold-diluted SW supplemented with 20 g.l(-1) glucose. However, the HV level (1.1-1.3 mol%) was very low. The component in SW contributing to copolymer production was found to be valerate. By 20 mM valerate 0.2 g.l(-1) PHBV with 44.6 mol% HV was produced. With 30 g.l(-1) glucose supplementation, 4.0 g.l(-1) PHBV with 22 mol% HV was produced. The optimal ratios of carbon to phosphorus (C : P) and to nitrogen (C : N) were 165 : 1 and 22 : 1, respectively.
Cho
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Department of Environmental Science and Engineering, Ewha Womans University, 11-1 Deahyon-dong, Seodaemum-gu, Seoul 120-750, Korea.
Ryu
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HW
Park
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Goodrich
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PR