title: Estrogen receptor alpha (ERalpha) deficiency in macrophages results in increased stimulation of CD4+ T cells while 17beta-estradiol acts through ERalpha to increase IL-4 and GATA-3 expression in CD4+ T cells independent of antigen presentation.
abstract: The effects of 17beta-estradiol (E2) on immune function have been extensively reported. The effects are dependent on concentration and duration of exposure and potential differences in signaling between the known E2 receptors, estrogen receptors (ER) alpha and ERbeta. Through the use of ER-deficient mice, we and others have begun to demonstrate the role of the two known receptors in modulating immune functional activities. Previous studies have shown that cells of the innate immune system have altered function (bactericidal capacity) and patterns of cytokine expression (increased proinflammatory cytokine expression) through amelioration of ERalpha signaling. In this study, we extend these studies to analysis of T cell differentiation and proliferation in APC-dependent and APC-independent in vitro assay systems. Our results demonstrate that ERalpha deficiency in splenic macrophages, but not CD11c+ splenic dendritic cells pulsed with OVA significantly enhances proliferative responses and IFN-gamma production by transgenic OVA peptide-specific (OT-II) CD4+ T cells when compared with Ag-pulsed APC from wild-type littermates. The addition of E2 in this culture system did not significantly affect the production of IFN-gamma. In addition, when purified CD4+ T cells from ERalpha-deficient and wild-type littermates were stimulated with anti-CD3/CD28 Ab in the absence of E2, there were no significant differences in IFN-gamma or IL-4 production. However, the addition of E2 significantly increased IL-4 secretion, as well as increased GATA-3 mRNA levels from ERalpha-replete CD4+ T cells, while this effect was abrogated in ERalpha-deficient CD4+ T cells.
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO 65211, USA.
authors.forename: K Chad
authors.initials: KC
authors.lastname: Lambert
authors.forename: Edward M
authors.initials: EM
authors.lastname: Curran
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Judy
authors.forename: Gregg N
authors.initials: GN
authors.lastname: Milligan
authors.forename: Dennis B
authors.initials: DB
authors.lastname: Lubahn
authors.forename: D Mark
authors.initials: DM
authors.lastname: Estes
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000172:deficiency / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D047629:Estrogen Receptor beta / Q000235:genetics; D050990:GATA3 Transcription Factor / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D016207:Cytokines; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D015847:Interleukin-4; D004958:Estradiol; D007371:Interferon-gamma