Determination of protein phosphorylation sites by mass spectrometry: a novel electrospray-based method.
Several methods are used to identify protein phosphorylation sites. We report a novel electrospray-based method for the determination of phosphorylation sites by mass spectrometry, using two different declustering potential values. This method allows one to obtain, with a single liquid chromatography/mass spectrometry (LC/MS) run, the pattern with either the phosphorylated or the unphosphorylated species of a protein tryptic digest, that can be further analyzed by tracing back the origin of each HPO3-deprived form using the capabilities of tandem mass spectrometers.
Alverdi
Vera
V
Department of Biomedical Sciences and Technologies, Center of Excellence M.A.T.I., University of Udine, P. le Kolbe 4, 33100 Udine, Italy.
Di Pancrazio
Francesca
F
Lippe
Giovanna
G
Pucillo
Carlo
C
Casetta
Bruno
B
Esposito
Gennaro
G
D000818:Animals; D002364:Caseins / Q000737:chemistry*; D002417:Cattle; D002853:Chromatography, Liquid; D010766:Phosphorylation; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D002364:Caseins