Characterization of lipoxygenase-1 null mutants in barley.
This study describes the discovery and characterization of lipoxygenase-1 (LOX-1) null mutants in barley. Six lines did not exhibit any significant LOX activity in the silenced seed extract. Immunological analysis showed that these lines lacked the authentic LOX-1 protein. Genetic analysis of the F2 population revealed that this trait was governed by a single recessive gene located at the LoxA locus on chromosome 4H. The six LOX-1 null mutants shared similar features and the same unique polymorphism in a structural gene region, implying that these mutants might be derived from the same ancestral origin.
Hirota
N
N
Bioresources Research and Development Laboratories, Sapporo Breweries Ltd., 37-1 Nitta-Kizaki, Ota, Gunma 370-0393, Japan. naohiko.hirota@sapporobeer.co.jp
Kaneko
T
T
Kuroda
H
H
Kaneda
H
H
Takashio
M
M
Ito
K
K
Takeda
K
K
D015139:Blotting, Southern; D015153:Blotting, Western; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D001467:Hordeum / Q000201:enzymology / Q000235:genetics*; D008084:Lipoxygenase / Q000235:genetics* / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012639:Seeds / Q000235:genetics* / Q000378:metabolism
D008084:Lipoxygenase