Clinostating effects on Apogee wheat resistance to wheat streak mosaic virus.
The effects of vertical and horizontal clinostating with container velocity 2 rpm and platform velocity 1 rpm on Apogee wheat resistance to wheat streak mosaic virus (WSMV) were studied. For the first time the yield of grain was obtained from both, healthy and the WSMV-infected wheat plants under conditions of simulated microgravity (clinorotation) which is accounted for by the inclusion of various mechanisms in the process of plant adaptation to the factors of stresses. For the first time the stages of viral infection development were elucidated under the conditions of prolonged clinostating and the presence of the WSMV was detected in wheat plants on the 18th day after inoculation employing the method of polymerase chain reaction (PCR). In the test variant with vertical clinostating (R=1,0) the least favorable conditions for viral reproduction were identified. In the same variant the increases of the total nitrogen content in the ears were observed which may be an evidence of interferon-like protein synthesis in plant similar to the interferon system functioning in animals.
Mishchenko
L T
LT
Taras Shevchenko Kiev National University, Kiev, Ukraine. lmishchenko@ukr.net
Filenko
O M
OM
Ostapchenko
L I
LI
Mishchenko
I A
IA
Yanishevska
G S
GS
Boyko
A L
AL
D018533:Biomass; D017343:Genes, Plant; D007113:Immunity, Innate / Q000276:immunology*; D009029:Mosaic Viruses / Q000276:immunology*; D009584:Nitrogen / Q000378:metabolism; D018515:Plant Leaves / Q000821:virology; D016133:Polymerase Chain Reaction; D012399:Rotation; D012639:Seeds; D014908:Triticum / Q000235:genetics / Q000254:growth & development / Q000821:virology*; D018474:Weightlessness Simulation
D009584:Nitrogen