New, rapid fluorescence stain method for histologic sections using lanthanide complexes.
A new stain method for histologic sections different from traditional hematoxylin and eosin (H&E) methodology used two newly developed stain reagents from the lanthanide series, the antibiotic ofloxacin chelate of europium(III) and the ciprofloxacin hydrochloride chelate of terbium(III), exhibiting excellent fluorescence. These complexes are unique due to their nontoxicity, attractive fluorescent properties, rapid staining, and high water solubility. In this article, we focus on the interactions between biomolecules and synthesized lanthanide complexes and on the staining effect applied for tissue staining.
Su
Yunlan
Y
State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing, China. suyl@mail.tsinghua.edu.cn
Xu
Yizhuang
Y
Yang
Limin
L
Yang
Jun
J
Weng
Shifu
S
Yu
Zhiwu
Z
Wu
Jinguang
J
D002939:Ciprofloxacin / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007642:Keratosis / Q000473:pathology; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D015242:Ofloxacin / Q000737:chemistry*; D017785:Photons; D012469:Salivary Glands / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D028581:Lanthanoid Series Elements; D002939:Ciprofloxacin; D015242:Ofloxacin