title: Apparent successful mesothelial cell transplantation hampered by peritoneal activation.
abstract: BACKGROUND
  Mesothelial cell transplantation has been suggested to improve mesothelial repair after surgery, recurrent peritonitis and peritoneal dialysis.
  
  
  METHODS
  In this study we evaluated mesothelial cell transplantation during the resolution phase of experimentally thioglycollate-induced peritonitis in rats. To this end 4 x 10(6) DiO-labeled autologous mesothelial cells were transplanted 1 week after peritonitis induction. Peritoneal inflammation and permeability characteristics were evaluated after another week.
  
  
  RESULTS
  Mesothelial cell transplantation after peritonitis resulted in incorporation of these cells in the parietal mesothelial lining, leading to an acute transient submesothelial thickening which was not seen in transplanted animals without prior peritonitis induction. Long-term functioning of these repopulated mesothelial cells leaded to peritoneal activation as evidenced by a approximately twofold increase in peritoneal lymphocytes (P < 0.01) and omental mast cell counts (P < 0.05), accompanied by the induction of inflammation markers monocyte chemoattractant protein-1 (MCP-1) (P < 0.01) and hyaluronan (P < 0.01) in the transplanted peritonitis group, but not in rats with peritonitis without mesothelial cell transplantation or in control rats without mesothelial cell transplantation (all four parameters P < 0.01). In addition, trapping of transplanted mesothelial cells in the milky spots of omental tissue and lymphatic stomata of the diaphragm both in control and thioglycollate rats seems to increase microvascular permeability, reflected by apparent increased diffusion rates of small solutes and proteins.
  
  
  CONCLUSIONS
  Altogether, our data underscore the importance of controlling peritoneal (patho)physiology and function in mesothelial transplantation protocols.
authors.affiliation: Department of Molecular Cell Biology and Immunology, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands.
authors.forename: Liesbeth H P
authors.initials: LH
authors.lastname: Hekking
authors.forename: Machteld M
authors.initials: MM
authors.lastname: Zweers
authors.forename: Eelco D
authors.initials: ED
authors.lastname: Keuning
authors.forename: Bas A J
authors.initials: BA
authors.lastname: Driesprong
authors.forename: Dirk R
authors.initials: DR
authors.lastname: de Waart
authors.forename: Robert H J
authors.initials: RH
authors.lastname: Beelen
authors.forename: Jacob
authors.initials: J
authors.lastname: van den Born
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000637:transplantation*; D004848:Epithelium; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000276:immunology; D009852:Omentum / Q000166:cytology / Q000276:immunology; D010537:Peritoneum / Q000166:cytology* / Q000276:immunology*; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D013864:Thioglycolates
chemical_list: D013864:Thioglycolates