title: Dynamics and spatial organization of endosomes in mammalian cells.
abstract: We combine particle tracking and stochastic simulations to analyze the dynamics and organization of early endocytic vesicles in mammalian cells. At short time scales (<10(1) sec) vesicles exhibit 1D symmetric bidirectional motor-driven transport on microtubules such that the mean squared displacement (MSD) scales as t3/2, but the MSD shows a crossover to facilitated diffusion at longer times (>10(1) sec). Facilitated diffusion results in rapid equilibration of vesicles on microtubules. The asterlike organization of microtubules causes perinuclear accumulation of vesicles despite symmetric transport.
authors.affiliation: Department of Chemical Engineering, University of California Santa Barbara, Santa Barbara, California 93106, USA.
authors.forename: Chinmay
authors.initials: C
authors.lastname: Pangarkar
authors.forename: Anh Tuan
authors.initials: AT
authors.lastname: Dinh
authors.forename: Samir
authors.initials: S
authors.lastname: Mitragotri
mesh_terms: D002478:Cells, Cultured; D003198:Computer Simulation; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000000981:diagnostic imaging* / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D009068:Movement / Q000502:physiology*; D014463:Ultrasonography