title: Analysis of estrogen-like compounds in the environment by high performance liquid chromatography bioassay.
abstract: To identify chemicals with endocrine-disrupting activity easily, we developed a new bioassay system, consisting of bioassay using genetically modified yeast expressing human estrogen receptor and high performance liquid chromatography (HPLC), in which advantages of instrumental analysis and bioassay are combined. The peaks in the mixture of these estrogen-like compounds analyzed using an HPLC bioassay were similar to those obtained by analysis using an HPLC-UV detector. Underground water and sea sediment were analyzed by an HPLC bioassay, and detected a few estrogen-like compounds, respectively. Estrogen-like compounds and yeast-growth inhibitors can be separated by HPLC-bioassay.
authors.affiliation: Mie Pref. Institute of Public Health & Environmental Science, 3690-1 Sakura, Yokkaichi, Mie 512-1211, Japan. iwasas00@pref.mie.jp
authors.forename: Seiji
authors.initials: S
authors.lastname: Iwasaki
authors.forename: Susumu
authors.initials: S
authors.lastname: Kato
authors.forename: Masaaki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kimura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
authors.forename: Kunio
authors.initials: K
authors.lastname: Ohmiya
authors.forename: Tomonari
authors.initials: T
authors.lastname: Matsuda
authors.forename: Saburo
authors.initials: S
authors.lastname: Matsui