title: Characterization of lipoxygenase-1 null mutants in barley.
abstract: This study describes the discovery and characterization of lipoxygenase-1 (LOX-1) null mutants in barley. Six lines did not exhibit any significant LOX activity in the silenced seed extract. Immunological analysis showed that these lines lacked the authentic LOX-1 protein. Genetic analysis of the F2 population revealed that this trait was governed by a single recessive gene located at the LoxA locus on chromosome 4H. The six LOX-1 null mutants shared similar features and the same unique polymorphism in a structural gene region, implying that these mutants might be derived from the same ancestral origin.
authors.affiliation: Bioresources Research and Development Laboratories, Sapporo Breweries Ltd., 37-1 Nitta-Kizaki, Ota, Gunma 370-0393, Japan. naohiko.hirota@sapporobeer.co.jp
authors.forename: N
authors.initials: N
authors.lastname: Hirota
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: H
authors.initials: H
authors.lastname: Kuroda
authors.forename: H
authors.initials: H
authors.lastname: Kaneda
authors.forename: M
authors.initials: M
authors.lastname: Takashio
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: K
authors.initials: K
authors.lastname: Takeda
mesh_terms: D015139:Blotting, Southern; D015153:Blotting, Western; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D001467:Hordeum / Q000201:enzymology / Q000235:genetics*; D008084:Lipoxygenase / Q000235:genetics* / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012639:Seeds / Q000235:genetics* / Q000378:metabolism
chemical_list: D008084:Lipoxygenase