Stabilization effect of zeolite on DHFR mRNA in a wheat germ cell-free protein synthesis system.
The effects of zeolites and monocations on the protein synthesis in a cell-free system derived from wheat germ were investigated. M type of synthetic zeolite markedly enhanced the translation efficiency. Whereas this kind of stimulatory effect of zeolite in an Escherichia coli cell-free system resulted from a change in the salt compositions of the reaction solution with the addition of zeolite, the enhancement of protein synthesis in a wheat germ cell-free system was not due to the ion exchange reaction of zeolites. From the results of mRNA stability analysis, it was found that zeolite could stabilize the mRNA in a wheat germ cell-free protein synthesis system. The stabilization of mRNA by the simple addition of zeolites is useful for the enhancement of protein synthesis in a wheat germ cell-free system, since conventional methods to improve mRNA stability, such as the addition of nuclease inhibitor, have not been effective for a wheat germ cell-free system.
Jung
G Y
GY
School of Chemical Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea.
Lee
E Y
EY
Kim
Y
Y
Jung
B W
BW
Kang
S H
SH
Choi
C Y
CY