Identification and organ expression of peroxisome proliferator activated receptors in brown trout (Salmo trutta f. fario).
Although widely studied in mammals, little information about fish peroxisome proliferator activated receptors (PPARs) is yet available. As a baseline for future studies, the three PPAR isotypes were identified in brown trout (Salmo trutta f. fario) and their organ distribution pattern was established. The cDNA fragments encoding PPARs alpha, beta and gamma were amplified by PCR, and the deduced sequences of the correspondent peptides were compared with other species sequences. Both the 183 amino acid sequence from PPARalpha and the 103 amino acid sequence from PPARbeta shared high levels of homology with the correspondent peptides of other fishes and terrestrial vertebrates, whereas PPARgamma 108 amino acid sequence showed much less similarity with non-fish PPARgamma. According to both semi-quantitative RT-PCR and real-time RT-PCR, PPARalpha mRNA predominates in white muscle, heart and liver and PPARbeta is more expressed in testis, heart, liver, white muscle and trunk kidney. PPARgamma was only detected in trunk kidney and liver by real-time RT-PCR and also in spleen by semi-quantitative RT-PCR. PPARbeta seems to be the most strongly expressed isotype, whereas PPARgamma shows a much weaker global expression.
Batista-Pinto
Carla
C
Laboratory of Cell Biology, ICBAS-Institute of Biomedical Sciences Abel Salazar, University of Porto, Portugal. carla@aeiou.pt
Rodrigues
Pedro
P
Rocha
Eduardo
E
Lobo-da-Cunha
Alexandre
A
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics*; D047492:Peroxisome Proliferator-Activated Receptors / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D014337:Trout / Q000235:genetics* / Q000378:metabolism
D047492:Peroxisome Proliferator-Activated Receptors