Biodesulfurization of dibenzothiophene with Rhodococcus erythropolis ATCC 53968 and its mutant in an interface bioreactor.
An interface bioreactor was used in the biodesulfurization (BDS) of dibenzothiophene (DBT) with Rhodococcus erythropolis ATCC 53968. Although this strain could efficiently degrade DBT to 2-hydroxybiphenyl (2-HBP), the microbial film peeled off from the surface of the carrier (agar plate) to an organic phase (dodecane or tetradecane). To overcome this problem, a UV mutant, which strongly adhered to the carrier surface, was obtained by a new screening method. The best UV mutant (UM-021) could efficiently catalyze the BDS and the microbial film did not peel off from the surface of the carrier to tetradecane layer.
Oda
Shinobu
S
Analytical Center, Kansai Paint Co., Ltd., 4-17-1 Higashi-Yawata, Hiratsuka, Kanagawa 254-8562, Japan. odas@als.kansai.co.jp
Ohta
Hiromichi
H