Degradation of 4-aminobenzenesulfonate by alginate encapsulated cells of Agrobacterium sp. PNS-1.
Studies were carried out on 4-aminobenzenesulfonate (4-ABS) degradation by free and alginate entrapped cells of Agrobacterium sp. PNS-1. Degradation rate in batch reactors with free cells was marginally higher than Ca-encapsulated cells. Comparison of Ca2+ and Ba2+ as gelling agents showed that 4-ABS removal rate was significantly less with Ba-alginate entrapped cells. Specific degradation rates, using linear regression analysis and based on the initial biomass in the beads, varied from 49.7 mg/mg biomass/h to 92.0 mg/mg biomass/h for Ca-alginate encapsulated cells for different initial 4-ABS concentrations ranging from 200 to 800 mg/L. UV spectra of the aliquots drawn at different time intervals from batch reactors did not show accumulation of any intermediate during degradation. Ca-alginate immobilized cells could be repeatedly reused upto five cycles without any loss of activity. Studies with packed bed reactors, operated in a semi-continuous mode, showed that this could be used for 4-ABS degradation.
Singh
Poonam
P
Biotechnology Laboratory, Department of Chemistry, I.I.T., Kanpur 208 016, India.
Mishra
L C
LC
Pandey
Anjali
A
Iyengar
Leela
L
D000464:Alginates / Q000494:pharmacology*; D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D018914:Cells, Immobilized / Q000378:metabolism; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D007700:Kinetics; D008863:Microspheres; D012231:Rhizobium / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D013425:Sulfanilic Acids / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods
D000464:Alginates; D006603:Hexuronic Acids; D013425:Sulfanilic Acids; C100016:4-sulfanilic acid; D020723:Glucuronic Acid