title: Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion.
abstract: A new method based on a combination of reverse transcription polymerase chain reaction (RT-PCR) and a water-in-oil (W/O) emulsion was developed. Reverse transcription and initial thermal cycles were carried out in droplets of the W/O emulsion. Then, the droplets were united, followed by remaining secondary PCR cycles. This two-step method succeeded in detecting a single RNA molecule.
authors.affiliation: Department of Ecological Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi, Aichi 441-8580, Japan.
authors.forename: Michihiko
authors.initials: M
authors.lastname: Nakano
authors.forename: Naohito
authors.initials: N
authors.lastname: Nakai
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kurita
authors.forename: Jun
authors.initials: J
authors.lastname: Komatsu
authors.forename: Kazunori
authors.initials: K
authors.lastname: Takashima
authors.forename: Shinji
authors.initials: S
authors.lastname: Katsura
authors.forename: Akira
authors.initials: A
authors.lastname: Mizuno
mesh_terms: D005591:Chemical Fractionation / Q000379:methods; D004655:Emulsions / Q000737:chemistry; D006678:HIV / Q000235:genetics* / Q000302:isolation & purification*; D009821:Oils / Q000737:chemistry*; D012367:RNA, Viral / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014867:Water / Q000737:chemistry*
chemical_list: D004655:Emulsions; D009821:Oils; D012367:RNA, Viral; D014867:Water