Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenic Aspergillus oryzae by PCR.
A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.
Honda
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M
Department of Plant Biotechnology, Gifu Prefectural Institute for Bioindustrial Technology, 3481-2 Kamihachiya, Hachiya-cho, Minokamo-shi, Gifu 505-0004, Japan.
Minetoki
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T
Gomi
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Machida
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M