title: Screening for and purification of novel self-aggregatable lectins reveal a new functional lectin group in the bark of leguminous trees.
abstract: A solubility-insolubility transition assay was used to screen the bark and stems of seven leguminous trees and plants for self-aggregatable lectins. Novel lectins were found in two trees, Robinia pseudoacacia and Wisteria floribunda, but not in the leguminous plants. The Robinia lectin was isolated from coexisting lectin by combined affinity chromatographies on various sugar adsorbents. The purified lectins proved to be differently glycosylated glycoproteins. One lectin exhibited the remarkable characteristics of self-aggregatable lectins: localization in the bark of legume trees, self-aggregation dissociated by N-acetylglucosamine/mannose, and coexistence with N-acetylgalactosamine/galactose-specific lectins, which are potential endogenous receptors. Self-aggregatable lectins are a functional lectin group that can link enhanced photosynthesis to dissociation of glycoproteins.
authors.affiliation: Department of Advanced Biosciences, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan.
authors.forename: Chieko
authors.initials: C
authors.lastname: Ina
authors.forename: Kotone
authors.initials: K
authors.lastname: Sano
authors.forename: Makiko
authors.initials: M
authors.lastname: Yamamoto-Takahashi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Matsushita-Oikawa
authors.forename: Hiroko
authors.initials: H
authors.lastname: Takekawa
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Takehara
authors.forename: Haruko
authors.initials: H
authors.lastname: Ueda
authors.forename: Haruko
authors.initials: H
authors.lastname: Ogawa
mesh_terms: D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007887:Fabaceae / Q000737:chemistry*; D007564:Japan; D009005:Monosaccharides / Q000378:metabolism; D024301:Plant Bark / Q000737:chemistry*; D037121:Plant Lectins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*; D011485:Protein Binding; D012995:Solubility
chemical_list: D009005:Monosaccharides; D037121:Plant Lectins