title: RACK1 mRNA translation is regulated via a rapamycin-sensitive pathway and coordinated with ribosomal protein synthesis.
abstract: RACK1 has been shown to interact with several proteins, this suggesting that it may play a central role in cell growth regulation. Some recent articles have described RACK1 as a component of the small ribosomal subunit. To investigate the relationship between RACK1 and ribosome, we analyzed RACK1 mRNA structure and regulation. Translational regulation was studied in HeLa cells subjected to serum or amino acid deprivation and stimulation. The results show that RACK1 mRNA has a 5' terminal oligopyrimidine sequence and that its translation is dependent on the availability of serum and amino acids in exactly the same way as any other vertebrate ribosomal protein mRNA.
authors.affiliation: Department of Biology, University Tor Vergata, Roma, Italy. loreni@uniroma2.it
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Loreni
authors.forename: Valentina
authors.initials: V
authors.lastname: Iadevaia
authors.forename: Elisa
authors.initials: E
authors.lastname: Tino
authors.forename: Sara
authors.initials: S
authors.lastname: Caldarola
authors.forename: Francesco
authors.initials: F
authors.lastname: Amaldi
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D014176:Protein Biosynthesis / Q000187:drug effects / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics; D020123:Sirolimus / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D009363:Neoplasm Proteins; C419368:RACK1 protein, human; D012333:RNA, Messenger; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D012269:Ribosomal Proteins; D019204:GTP-Binding Proteins; D020123:Sirolimus