[Role of chemokines in the vitreous of proliferative diabetic retinopathy].
OBJECTIVE
To investigate the role of chemokines in the pathogenesis of proliferative diabetic retinopathy (PDR).


METHODS
In total, 41 eyes of 38 patients undergoing vitrectomy were divided into two groups; PDR and non-PDR. The PDR group was comprised of 30 eyes, and the non-PDR group of 11 eyes. Vitreous specimens obtained at vitrectomy were centrifuged and separated into supernatants and cellular components. Concentrations of vascular endothelial growth factor(VEGF), interleukin-8 (IL-8), monocyte chemotactic protein-1 (MCP-1), and regulated upon activation, normal T cell expressed and secreted(RANTES) in the supernatants were determined by enzyme-linked immunosorbent assay(ELISA) or chemiluminescence enzyme immunoassay(CLEIA). Expression of VEGF in the cellular components was determined by immunohistochemistry.


RESULTS
Vitreous levels of VEGF(p < 0.05), IL8 (p < 0.0001) and MCP-1 (p < 0.05) in the PDR group were significantly higher than in the non-PDR group. However, there was no significant difference in RANTES between the two groups. There was a significant correlation (p < 0.0001, r = 0.84) between vitreous IL-8 and MCP-1 levels in the PDR group. After immunohistochemical staining with antiVEGF monoclonal antibody, VEGF positivity was localized in polymorphonuclear leukocytes and monocytes of the cellular components of PDR vitreous specimens.


CONCLUSIONS
These results indicate that chemokines are possibly involved in the recruitment of neutrophils and monocytes into the vitreous and that they play a role in the intraocular neovascularization characteristic of PDR.
Kawashima
Miwako
M
Department of Ophthalmology, Nihon University, School of Medicine, 30-1 Oyaguchikami-machi, Itabashiku-ku, Tokyo 173-8610, Japan.
Shoji
Jun
J
Kamura
Yumi
Y
Sato
Yukihiro
Y
D018932:Chemokine CCL2 / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism; D018925:Chemokines / Q000502:physiology*; D003930:Diabetic Retinopathy / Q000209:etiology* / Q000378:metabolism; D016228:Endothelial Growth Factors / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D015861:Retinal Neovascularization / Q000378:metabolism; D014821:Vitrectomy; D014822:Vitreous Body / Q000378:metabolism*
D018932:Chemokine CCL2; D018946:Chemokine CCL5; D018925:Chemokines; D016228:Endothelial Growth Factors; D016209:Interleukin-8