Optimisation and robustness analysis of a hydrophobic interaction chromatography step.
Process development, optimisation and robustness analysis for chromatography separations are often entirely based on experimental work and generic knowledge. The present study proposes a method of gaining process knowledge and assisting in the robustness analysis and optimisation of a hydrophobic interaction chromatography step using a model-based approach. Factorial experimental design is common practice in industry today for robustness analysis. The method presented in this study can be used to find the critical parameter variations and serve as a basis for reducing the experimental work. In addition, the calibrated model obtained with this approach is used to find the optimal operating conditions for the chromatography column. The methodology consists of three consecutive steps. Firstly, screening experiments are performed using a factorial design. Secondly, a kinetic-dispersive model is calibrated using gradient elution and column load experiments. Finally, the model is used to find optimal operating conditions and a robustness analysis is conducted at the optimal point. The process studied in this work is the separation of polyclonal IgG from BSA using hydrophobic interaction chromatography.
Jakobsson
Niklas
N
Department of Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
Degerman
Marcus
M
Nilsson
Bernt
B
D000327:Adsorption; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D008962:Models, Theoretical