Formation of large DNA aggregates induced by spermidine.
The formation of large DNA aggregates induced by spermidine was investigated by UV absorptiometry and polarizing microscopy. The present results reveal that it is stepwise and involves the following morphological variations: fiber, fiber bundles, and a highly condensed phase. Furthermore, the influence of DNA concentration on not only the spermidine concentration required for the DNA aggregation but also the concentration of free spermidine during the aggregation is analyzed.
Kuramochi
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SVBL, Graduate School of Science and Engineering, Ibaraki University, 4-12-1 Nakanarusawa, Hitachi, Ibaraki 316-8511, Japan. kuramochi.hidetoshi@nies.go.jp
Yonezawa
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