Genetic polymorphism of alcohol dehydrogenase 3 in alcohol liver cirrhosis and in alcohol chronic pancreatitis.
OBJECTIVE
To find the ADH3 genotypes in the Polish population likely to be responsible for higher susceptibility to alcohol disease of the liver and chronic alcohol pancreatitis.


METHODS
The ADH3 genotype and ADH3*1 and ADH3*2 alleles frequencies were examined in 198 patients. Genotyping of the ADH3 was performed using PCR-restriction fragment length polymorphism methods on a white cell DNA.


RESULTS
The genotype ADH3*1/ADH3*1 was found to be significantly more frequent in alcohol abusers compared with non-drinkers. The examinations of the group of alcohol abusers showed that the genotype ADH3*2/ADH3*2 occurred statistically significantly less frequently in patients with chronic pancreatitis than in those without alimentary lesions (healthy drinkers). The alleles ADH3*1 and genotype ADH3*1/ADH3*1 were significantly more frequent in men than in women, whereas alleles ADH3*2 and genotype ADH3*2/ADH3*2 were more common in women.


CONCLUSIONS
The genotype ADH3*2/ADH3*2 is likely to be a protective factor for chronic pancreatitis. Variations in ADH3 genotypes may account for some of the differences in prevalence of alcohol dependence between genders in the Polish population.
Cichoz-Lach
Halina
H
Department of Gastroenterology and 2 Department of Medical Genetics, Medical University of Lublin, ul. Jaczewskiego 8, Lublin, Poland. Lach.Halina@wp.pl
Partycka
Jadwiga
J
Nesina
Irina
I
Celinski
Krzysztof
K
Słomka
Maria
M
Wojcierowski
Jacek
J
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000483:Alleles; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008104:Liver Cirrhosis, Alcoholic / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D019512:Pancreatitis, Alcoholic / Q000235:genetics*; D050500:Pancreatitis, Chronic / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics
D017931:DNA Primers; C420272:ADH1C protein, human; D000426:Alcohol Dehydrogenase