Rapid amplification of a water channel-like gene and its flanking sequences from the Methanothermobacter marburgensis genome using a single primer PCR strategy.
In this study, we isolated a water channel-like gene and its flanking sequences from an archaeal genome using only a set of PCR. This method should greatly simplify gene cloning procedures and improve the efficiency of cloning.
Ding
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Biotechnology Institute, Akita Prefectural University, Ogata, Akita 010-04, Japan.
Kitagawa
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