Detection of human herpesviruses and polyomaviruses DNA in a group of patients with relapsing-remitting multiple sclerosis.
Multiple sclerosis (MS) is an inflammatory disease of the central nervous system whose pathological features consist of white matter plaques of primary demyelinization and loss of oligodendrocytes. Various risk factors have been associated with MS susceptibility. We have focused this study on different viruses. In particular in the present study we used PCR to search for the genomic DNA of HHV-1, HHV-2, HHV-8, BKV and JCV in urine and peripheral blood mononuclear cells (PBMC) samples from 44 relapsing-remitting MS (RRMS) patients. No viral DNA was found in any urine sample, whereas 29.5% of RRMS PBMC samples were positive. It is suggestive that Human herpesviruses (HHV-1 and HHV-8) were constantly present in all positive samples, indicating that viral agents could contribute to create the demyelination plaques and cause MS.
Pietropaolo
Valeria
V
Department of Public Health Sciences, La Sapienza University, Rome, Italy. valeria.pietropaolo@uniroma1.it
Fioriti
Daniela
D
Mischitelli
Monica
M
Anzivino
Elena
E
Santini
Manuela
M
Millefiorini
Enrico
E
Di Rezze
Simone
S
Degener
Anna Marta
AM
D000293:Adolescent; D000328:Adult; D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D006566:Herpesviridae Infections / Q000150:complications; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000150:complications / Q000821:virology*; D016133:Polymerase Chain Reaction; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000150:complications; D018139:Simplexvirus / Q000235:genetics / Q000302:isolation & purification*; D014412:Tumor Virus Infections / Q000150:complications; D014556:Urine / Q000821:virology
D004279:DNA, Viral