Whole-genome sequencing of staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species.
Staphylococcus haemolyticus is an opportunistic bacterial pathogen that colonizes human skin and is remarkable for its highly antibiotic-resistant phenotype. We determined the complete genome sequence of S.haemolyticus to better understand its pathogenicity and evolutionary relatedness to the other staphylococcal species. A large proportion of the open reading frames in the genomes of S.haemolyticus, Staphylococcus aureus, and Staphylococcus epidermidis were conserved in their sequence and order on the chromosome. We identified a region of the bacterial chromosome just downstream of the origin of replication that showed little homology among the species but was conserved among strains within a species. This novel region, designated the "oriC environ," likely contributes to the evolution and differentiation of the staphylococcal species, since it was enriched for species-specific nonessential genes that contribute to the biological features of each staphylococcal species. A comparative analysis of the genomes of S.haemolyticus, S.aureus, and S.epidermidis elucidated differences in their biological and genetic characteristics and pathogenic potentials. We identified as many as 82 insertion sequences in the S.haemolyticus chromosome that probably mediated frequent genomic rearrangements, resulting in phenotypic diversification of the strain. Such rearrangements could have brought genomic plasticity to this species and contributed to its acquisition of antibiotic resistance.
Takeuchi
Fumihiko
F
Department of Microbiology and Infection Control Science, Juntendo University, Tokyo, Japan.
Watanabe
Shinya
S
Baba
Tadashi
T
Yuzawa
Harumi
H
Ito
Teruyo
T
Morimoto
Yuh
Y
Kuroda
Makoto
M
Cui
Longzhu
L
Takahashi
Mikio
M
Ankai
Akiho
A
Baba
Shin-ichi
S
Fukui
Shigehiro
S
Lee
Jean C
JC
Hiramatsu
Keiichi
K
D005075:Biological Evolution; D002876:Chromosomes, Bacterial / Q000235:genetics; D017124:Conserved Sequence; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D023061:Gene Order; D015321:Gene Rearrangement; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013211:Staphylococcus aureus / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000235:genetics; D041162:Staphylococcus haemolyticus / Q000235:genetics* / Q000472:pathogenicity; D026801:Synteny; D014774:Virulence / Q000235:genetics
D004251:DNA Transposable Elements; D004269:DNA, Bacterial