Analysis tissue expression of IFN-gamma in IL-12 and/or IL-18 gene ablated naïve mice.
Interleukin 12 (IL-12) and/or interleukin 18 (IL-18) gene ablated mice were applied for the investigation of the tissue expression of interferon gamma (IFN-gamma). For IL-12(-/-), IL-18(-/-), IL-12(-/-)/18(-/-) and wt mice, reproductive performance were recorded and IFN-gamma concentrations in heart, lung, liver, spleen, kidney and serum were quantified by ELISA. There were no significant differences of IFN-gamma in heart, lung and kidney between 4 strains although control group was higher. It was observed that for IL-12(-/-) mice, compared with other 3 groups, IFN-gamma in liver and spleen were decreased (p < 0.05) and reproductive performance appeared to be impaired. Serum IFN-gamma level of IL-12(-/-)/18(-/-) mice was significantly higher (p < 0.05). It was showed that IFN-gamma productions under the normal condition were independent upon IL-12 and IL-18, its expressions in various tissues were different, and optimal IFN-gamma is necessary for the normal growth and development of mammals. This study is helpful for clinical cytokines therapy.
Zhang
Jian Hong
JH
Institute of Immunology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China.
Wei
Hai Ming
HM
Tian
Zhi Gang
ZG
D000818:Animals; D002457:Cell Extracts; D005786:Gene Expression Regulation / Q000235:genetics; D007371:Interferon-gamma / Q000378:metabolism*; D018664:Interleukin-12 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D020382:Interleukin-18 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009928:Organ Specificity
D002457:Cell Extracts; D020382:Interleukin-18; D018664:Interleukin-12; D007371:Interferon-gamma