title: The HOX genes are expressed, in vivo, in human tooth germs: in vitro cAMP exposure of dental pulp cells results in parallel HOX network activation and neuronal differentiation.
abstract: Homeobox-containing genes play a crucial role in odontogenesis. After the detection of Dlx and Msx genes in overlapping domains along maxillary and mandibular processes, a homeobox odontogenic code has been proposed to explain the interaction between different homeobox genes during dental lamina patterning. No role has so far been assigned to the Hox gene network in the homeobox odontogenic code due to studies on specific Hox genes and evolutionary considerations. Despite its involvement in early patterning during embryonal development, the HOX gene network, the most repeat-poor regions of the human genome, controls the phenotype identity of adult eukaryotic cells. Here, according to our results, the HOX gene network appears to be active in human tooth germs between 18 and 24 weeks of development. The immunohistochemical localization of specific HOX proteins mostly concerns the epithelial tooth germ compartment. Furthermore, only a few genes of the network are active in embryonal retromolar tissues, as well as in ectomesenchymal dental pulp cells (DPC) grown in vitro from adult human molar. Exposure of DPCs to cAMP induces the expression of from three to nine total HOX genes of the network in parallel with phenotype modifications with traits of neuronal differentiation. Our observations suggest that: (i) by combining its component genes, the HOX gene network determines the phenotype identity of epithelial and ectomesenchymal cells interacting in the generation of human tooth germ; (ii) cAMP treatment activates the HOX network and induces, in parallel, a neuronal-like phenotype in human primary ectomesenchymal dental pulp cells.
authors.affiliation: Department of Clinical and Experimental Medicine, Via S. Pansini 5, 80131, Naples, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: D'Antò
authors.forename: Monica
authors.initials: M
authors.lastname: Cantile
authors.forename: Maria
authors.initials: M
authors.lastname: D'Armiento
authors.forename: Giulia
authors.initials: G
authors.lastname: Schiavo
authors.forename: Gianrico
authors.initials: G
authors.lastname: Spagnuolo
authors.forename: Luigi
authors.initials: L
authors.lastname: Terracciano
authors.forename: Raffaela
authors.initials: R
authors.lastname: Vecchione
authors.forename: Clemente
authors.initials: C
authors.lastname: Cillo
mesh_terms: D000293:Adolescent; D000328:Adult; D002454:Cell Differentiation; D000242:Cyclic AMP; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008963:Molar / Q000378:metabolism*; D009474:Neurons / Q000502:physiology*; D016133:Polymerase Chain Reaction; D014083:Tooth Germ / Q000033:anatomy & histology / Q000196:embryology / Q000378:metabolism*
chemical_list: D018398:Homeodomain Proteins; D000242:Cyclic AMP