title: A comparison of accelerated solvent extraction, Soxhlet extraction, and ultrasonic-assisted extraction for analysis of terpenoids and sterols in tobacco.
abstract: The performance of accelerated solvent extraction in the analysis of terpenoids and sterols in tobacco samples was investigated and compared with those of Soxhlet extraction and ultrasonically assisted extraction with respect to yield, extraction time, reproducibility and solvent consumption. The results indicate that although the highest yield was achieved by Soxhlet extraction, ASE appears to be a promising alternative to classical methods since it is faster and uses less solvent, especially when applied to the investigation of large batch tobacco samples. However, Soxhlet extraction is still the preferred method for analyzing sterols since it gives a higher extraction efficiency than other methods.
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
authors.forename: Jinchao
authors.initials: J
authors.lastname: Shen
authors.forename: Xueguang
authors.initials: X
authors.lastname: Shao
mesh_terms: D000473:Alkanes / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D012997:Solvents / Q000737:chemistry*; D013261:Sterols / Q000032:analysis*; D013729:Terpenes / Q000032:analysis*; D014026:Nicotiana / Q000737:chemistry*; D014465:Ultrasonics
chemical_list: D000473:Alkanes; D012997:Solvents; D013261:Sterols; D013729:Terpenes; C004544:naphtha