title: The pathogenesis of and susceptibility to malabsorption syndrome in broilers is associated with heterophil influx into the intestinal mucosa and epithelial apoptosis.
abstract: Malabsorption syndrome (MAS) in broilers is characterized by enteritis and reduced body weight gain. The pathogenesis of the intestinal lesions and the reasons for susceptibility differences between broiler lines are not clear. We studied the development of enteric lesions, epithelial apoptosis, and cell proliferation in relation to susceptibility. One-day-old chickens from two broiler lines were orally inoculated with intestinal homogenate derived from MAS-affected chickens. Vacuolar degeneration and apoptosis of the villous epithelium and infiltration of heterophils into the lamina propria occurred from day 1 post-inoculation. Following heterophil accumulation, at day 4 to 6 post-inoculation, there was severe apoptosis of the crypt epithelium and villous atrophy. The susceptible broilers had a significantly greater influx of heterophils and, subsequently, severe epithelial apoptosis and cystic damage to the crypts. There appeared to be a causal relationship between heterophil influx and the onset of apoptosis. Coincident with the epithelial apoptosis, MAS-affected chickens had crypt hyperproliferation and faster epithelial turnover. Heterophil infiltration and epithelial apoptosis appear to be critical in the pathogenesis of MAS. Heterophil recruitment may be a major factor in differences in susceptibility to MAS.
authors.affiliation: Animal Sciences Group, Wageningen UR, Division of Animal Resources Development, P.O. Box 65, 8200, AB Lelystad, The Netherlands.
authors.forename: Bereket
authors.initials: B
authors.lastname: Zekarias
authors.forename: Norbert
authors.initials: N
authors.lastname: Stockhofe-Zurwieden
authors.forename: Jacob
authors.initials: J
authors.lastname: Post
authors.forename: Francis
authors.initials: F
authors.lastname: Balk
authors.forename: Cees
authors.initials: C
authors.lastname: van Reenen
authors.forename: Erik
authors.initials: E
authors.lastname: Gruys
authors.forename: Johanna M J
authors.initials: JM
authors.lastname: Rebel
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001837:Body Weights and Measures; D002645:Chickens; D004198:Disease Susceptibility / Q000662:veterinary*; D006652:Histological Techniques / Q000662:veterinary; D020287:In Situ Nick-End Labeling / Q000662:veterinary; D007413:Intestinal Mucosa / Q000502:physiology*; D008286:Malabsorption Syndromes / Q000473:pathology / Q000662:veterinary*; D011201:Poultry Diseases / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen
chemical_list: D018809:Proliferating Cell Nuclear Antigen