Rapid detection of glycyrrhizin by immunochromatographic assay.
An immunochromatographic assay was developed for detecting glycyrrhizin (1). The qualitative assay is based on a competitive immunoassay using anti-1 monoclonal antibody (MAb) and a detector reagent that contains colloidal gold particles coated with anti-1 MAb. The immunochromatographic strip test, which has a detection limit of 250 ng/mL, is useful as a rapid screening method for detecting glycyrrhizin in plants, biological fluids and food samples.
Putalun
Waraporn
W
Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand.
Tanaka
Hiroyuki
H
Shoyama
Yukihiro
Y
D000911:Antibodies, Monoclonal / Q000276:immunology; D002855:Chromatography, Thin Layer / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis; D006035:Glycyrrhiza / Q000737:chemistry; D019695:Glycyrrhizic Acid / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D019695:Glycyrrhizic Acid