A biosensor in the time domain based on the diffusion coefficient measurement: intermolecular interaction of an intermediate of photoactive yellow protein.
A new type of biosensor is presented for the first time. This method is based on the diffusion measurement in the time domain by the transient grating method. As the first demonstration of this new method, various intermolecular interactions with a reaction intermediate of photoactive yellow protein, such as the protein-protein interaction, protein-DNA interaction, and protein-small molecule binding are detected. The characteristic advantages and limitations are summarized.
Khan
Javaid Shahbaz
JS
Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Imamoto
Yasushi
Y
Yamazaki
Yoichi
Y
Kataoka
Mikio
M
Tokunaga
Fumio
F
Terazima
Masahide
M
D001426:Bacterial Proteins / Q000737:chemistry* / Q000502:physiology; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry / Q000502:physiology; D004058:Diffusion; D020283:Photoreceptors, Microbial / Q000737:chemistry* / Q000502:physiology; D011485:Protein Binding; D012680:Sensitivity and Specificity; D013997:Time Factors
D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; C050162:photoactive yellow protein, Bacteria; D004247:DNA