title: Analysis tissue expression of IFN-gamma in IL-12 and/or IL-18 gene ablated naïve mice.
abstract: Interleukin 12 (IL-12) and/or interleukin 18 (IL-18) gene ablated mice were applied for the investigation of the tissue expression of interferon gamma (IFN-gamma). For IL-12(-/-), IL-18(-/-), IL-12(-/-)/18(-/-) and wt mice, reproductive performance were recorded and IFN-gamma concentrations in heart, lung, liver, spleen, kidney and serum were quantified by ELISA. There were no significant differences of IFN-gamma in heart, lung and kidney between 4 strains although control group was higher. It was observed that for IL-12(-/-) mice, compared with other 3 groups, IFN-gamma in liver and spleen were decreased (p < 0.05) and reproductive performance appeared to be impaired. Serum IFN-gamma level of IL-12(-/-)/18(-/-) mice was significantly higher (p < 0.05). It was showed that IFN-gamma productions under the normal condition were independent upon IL-12 and IL-18, its expressions in various tissues were different, and optimal IFN-gamma is necessary for the normal growth and development of mammals. This study is helpful for clinical cytokines therapy.
authors.affiliation: Institute of Immunology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China.
authors.forename: Jian Hong
authors.initials: JH
authors.lastname: Zhang
authors.forename: Hai Ming
authors.initials: HM
authors.lastname: Wei
authors.forename: Zhi Gang
authors.initials: ZG
authors.lastname: Tian
mesh_terms: D000818:Animals; D002457:Cell Extracts; D005786:Gene Expression Regulation / Q000235:genetics; D007371:Interferon-gamma / Q000378:metabolism*; D018664:Interleukin-12 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D020382:Interleukin-18 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009928:Organ Specificity
chemical_list: D002457:Cell Extracts; D020382:Interleukin-18; D018664:Interleukin-12; D007371:Interferon-gamma