Isolation of aryl-phosphate ester-degrading bacterium from leachate of a sea-based waste disposal site.
An aryl-phosphate ester (APE)-degrading bacterium was isolated from the leachate of a sea-based waste disposal site. The isolated APE-degrading bacterial strain YS-57 grew well in a medium containing glucose and NaCl, and degraded two types of APE:tricresyl phosphate and triphenyl phosphate. The optimal temperature, pH, and NaCl concentration for the growth of strain YS-57 were 30 degrees C, 7.0, and 1.0%, respectively. Strain YS-57 grew at an APE concentration of 25 mg/l without being inhibited. APEs were degraded by the supernatant of the medium in which strain YS-57 was cultured, suggesting that the APE-degrading enzyme was released into the extracellular space in the logarithmic growth phase. The 16S rDNA sequence of strain YS-57 showed 95.6% similarity to that of Roseobacter gallaeciensis and the morphological properties were also comparable. Consequently, strain YS-57 was closely related to the genus Roseobacter.
Kawagoshi
Yasunori
Y
Department of Civil and Environmental Engineering, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan. goshi@kumamoto-u.ac.jp
Nakamura
Sachiko
S
Nishio
Takayuki
T
Fukunaga
Isao
I