Vibrionaceae as a possible source of Qnr-like quinolone resistance determinants.
OBJECTIVE
To gain insight into the functionality of qnr-like genes of several bacterial species of Vibrionaceae that may encode quinolone resistance determinants.


METHODS
A PCR-based strategy was used to obtain qnr-like genes of reference strains of Vibrio vulnificus CIP103196, Vibrio parahaemolyticus CIP71.2 and Photobacterium profundum CIP106289 that were sequenced, cloned and expressed in Escherichia coli. MICs of quinolones were determined for these reference strains and an E. coli reference strain harbouring recombinant plasmids.


RESULTS
The Qnr-like proteins of these Vibrionaceae bacterial species shared 40-67% amino acid identity with the plasmid-mediated quinolone resistance determinants QnrA, QnrB and QnrS with a series of highly conserved residues. Once cloned in E. coli they conferred reduced susceptibility to quinolones.


CONCLUSIONS
This work provides further evidence that water-borne Vibrionaceae may constitute a reservoir for Qnr-like quinolone resistance determinants.
Poirel
Laurent
L
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 K.-Bicêtre Cedex, France.
Liard
Alain
A
Rodriguez-Martinez
Jose-Manuel
JM
Nordmann
Patrice
P
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010776:Photobacterium / Q000187:drug effects / Q000235:genetics; D016133:Polymerase Chain Reaction; D015363:Quinolones / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014736:Vibrio parahaemolyticus / Q000187:drug effects / Q000235:genetics; D041261:Vibrio vulnificus / Q000187:drug effects / Q000235:genetics; D014737:Vibrionaceae / Q000187:drug effects* / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D015363:Quinolones