TGF-beta and TNF-alpha producing effects of losartan and amlodipine on human mononuclear cell culture.
OBJECTIVE
The modulation of cytokine release, which affects adhesion of leucocytes to endothelial cells, and proliferation of peripheral blood mononuclear cells with antihypertensive drugs was explored.


METHODS
In the present study, mononuclear cells were incubated with losartan and amlodipine at concentrations of 10(-6), 10(-5) and 10(-4) mol/L for 6 h. Transforming growth factor (TGF)-beta and tumour necrosis factor (TNF)-alpha levels were measured. Proliferation of mononuclear cells were assessed at the same concentrations of amlodipine and losartan with the methylthiazoletetrazolium (MTT) test.


RESULTS
Amlodipine was found to induce TGF-beta synthesis from mononuclear cells with increasing concentrations, while it was found to inhibit TNF-alpha secretion with increasing concentrations. In contrast, losartan was found to induce TGF-beta and TNF-alpha secretion with increasing concentrations.


CONCLUSIONS
Anti-atherosclerotic effects of amlodipine and losartan might be through increased secretion of TGF-beta from mononuclear cells. Different results at different concentrations might be due to the pharmocokinetic differences of these drugs.
Kaynar
Kubra
K
Department of Nephrology, School of Medicine, Karadeniz Technical University, Trabzon, Turkey.
Ulusoy
Sukru
S
Ovali
Ercument
E
Vanizor
Birgul
B
Dikmen
Tamer
T
Gul
Semih
S
D017311:Amlodipine / Q000494:pharmacology*; D000959:Antihypertensive Agents / Q000494:pharmacology*; D050197:Atherosclerosis / Q000188:drug therapy / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019808:Losartan / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000959:Antihypertensive Agents; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D017311:Amlodipine; D019808:Losartan