title: An enhanced method for peptides sequencing by N-terminal derivatization and MS.
abstract: An improved method for peptide sequencing based on acetylation/deuteroacetylation in conjunction with ESI MS is introduced. Derivatization with a 1:1 mixture of acetic anhydride and deuterated acetic anhydride incorporates a stable isotope label into the analyzed molecule. This approach has been initially applied to FAB. Using MS/MS, the technique provides a fast, highly sensitive and reliable determination of the primary structure of unknown peptides. This procedure labels N-terminal fragments formed during MS/MS analysis, resulting in a simplification and faster interpretation of the spectra. The performance of the method has been tested with several synthetic peptides and applied to an efficient sequencing of the peptide map, using a nano-scale LC coupled on-line to a tandem mass spectrometer.
authors.affiliation: Department of Neurobiochemistry, Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Krakow, Poland.
authors.forename: Marek J
authors.initials: MJ
authors.lastname: Noga
authors.forename: Jakub J
authors.initials: JJ
authors.lastname: Lewandowski
authors.forename: Piotr
authors.initials: P
authors.lastname: Suder
authors.forename: Jerzy
authors.initials: J
authors.lastname: Silberring
mesh_terms: D000107:Acetylation; D045304:Cytochromes c / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D009211:Myoglobin / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods; D010455:Peptides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014357:Trypsin / Q000737:chemistry
chemical_list: D009211:Myoglobin; D010446:Peptide Fragments; D010455:Peptides; D045304:Cytochromes c; D014357:Trypsin