title: Crystallographic studies of Mycobacterium tuberculosis polyphosphate/ATP-NAD kinase complexed with NAD.
abstract: NAD kinase from Mycobacterium tuberculosis (Ppnk) uses ATP or inorganic polyphosphate [poly(P)]. Ppnk overexpressed in Escherichia coli was purified and crystallized in the presence of NAD. Preliminary X-ray analysis of the resultant crystal indicate that the crystal belongs to hexagonal space group P6(2)22 and is holo-Ppnk complexed with NAD.
authors.affiliation: Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Shigetarou
authors.initials: S
authors.lastname: Mori
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Yukie
authors.initials: Y
authors.lastname: Maruyama
authors.forename: Keiko
authors.initials: K
authors.lastname: Momma
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kawai
authors.forename: Wataru
authors.initials: W
authors.lastname: Hashimoto
authors.forename: Bunzo
authors.initials: B
authors.lastname: Mikami
authors.forename: Kousaku
authors.initials: K
authors.lastname: Murata