[Cytotoxic effect of cisplatin with proteasome inhibitor on osteosarcoma cells].
To observe the cytotoxic effect of cisplatin with proteasome inhibitor on osteosarcoma cells.



Cell survival was tested by MTT, apoptotic morphology was observed by electron microscopy, apoptotic rate was analyzed by flow cytometry, the transcription level of excision repair cross complementation-1 (ERCC-1) was tested by reverse transcription polymerase reaction.



Compared with cells treated with cisplatin alone, cells treated with cisplatin and proteasome inhibitor showed a decreased survival rate, more typical apoptotic morphology, higher apoptotic rate [(14.37 +/-2.37)% vs. (50.93 +/-4.84)%, P<0.01)], and lower transcription level of excision repair cross complementation-1.



Proteasome inhibitor could increase the cytotoxic effect of cisplatin on osteosarcoma cells and promote cisplatin-induced osteosarcoma apoptosis. These effects may be associated with the decreased transcription of excision repair cross complementation-1.
Yang
Di-sheng
DS
The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China. dishengy@sohu.com
Li
Gao-shun
GS
Ye
Zhao-ming
ZM
Feng
Jie
J
Gao
Xiang
X
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000473:pathology*; D002945:Cisplatin / Q000494:pharmacology*; D004357:Drug Synergism; D009842:Oligopeptides / Q000494:pharmacology*; D012516:Osteosarcoma / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D009842:Oligopeptides; C090535:benzyloxycarbonyl-isoleucyl-glutamyl(O-tert-butyl)-alanyl-leucinal; D002945:Cisplatin