Molecular characterization of grapevine plants transformed with GFLV resistance genes: I.
The Grapevine FanLeaf Virus-Coat Protein (GFLV CP) gene was inserted through Agrobacterium-mediated transformation in Vitis vinifera "Nebbiolo", "Lumassina" and "Blaufränkisch". Two plasmids were used: pGA-CP+ (full-length GFLV CP gene with an introduced start codon) and pGA-AS (same gene in antisense orientation). Forty-three transgenic lines were regenerated. As several lines in Southern blots share same hybridization patterns, eight independent line groups resulted for "Nebbiolo", one for "Lumassina", and two for "Blaufränkisch". Inserted T-DNA copies ranged from one to three; one line probably contains an incomplete copy of T-DNA. Except for one "Nebbiolo" line, no evidence for methylation of the transgene at cytosine residues was found by Southern analyses. Specific mRNA was present at variable expression levels; some lines accumulated the coat protein while in others the protein was not detectable by ELISA.
Gambino
Giorgio
G
Istituto Virologia Vegetale C.N.R.-Unità staccata Viticoltura-Grugliasco, Via Leonardo da Vinci 44, I-10095 Grugliasco (Turin), Italy.
Gribaudo
Ivana
I
Leopold
Stephan
S
Schartl
Angelica
A
Laimer
Margit
M
D015139:Blotting, Southern; D018744:DNA, Plant; D015870:Gene Expression; D007113:Immunity, Innate / Q000235:genetics*; D010942:Plant Viruses / Q000276:immunology / Q000502:physiology*; D010944:Plants / Q000033:anatomy & histology / Q000196:embryology / Q000235:genetics* / Q000821:virology*; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics
D018744:DNA, Plant; D012333:RNA, Messenger