Production of protein kinase C-delta by the baculovirus-insect cell system in serum-supplemented and serum-free media.
When rat protein kinase C-delta (PKC-delta) was produced by Sf9 cells infected with a recombinant baculovirus in shake-flask culture using a serum-containing medium, the intracellular PKC-delta content decreased in the late period while the extracellular PKC-6 markedly increased. During the late period of serum-free culture, the extracellular PKC-6 level considerably declined, but the addition of a protease inhibitor, leupeptin, prevented the reduction in PKC-delta production.
Yamaji
Hideki
H
Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. yamaji@cx.kobe-u.ac.jp
Hirakawa
Daigo
D
Tagai
Shin-Ichi
S
Fukuda
Hideki
H