title: Proteolysis of mitotic chromosomes induces gradual and anisotropic decondensation correlated with a reduction of elastic modulus and structural sensitivity to rarely cutting restriction enzymes.
abstract: The effect of nonspecific proteolysis on the structure of single isolated mitotic newt chromosomes was studied using chromosome elastic response as an assay. Exposure to either trypsin or proteinase K gradually decondensed and softened chromosomes but without entirely eliminating their elastic response. Analysis of chromosome morphology revealed anisotropic decondensation upon digestion, with length increasing more than width. Prolonged protease treatment resulted only in further swelling of the chromosome without complete dissolution. Mild trypsinization induced sensitivity of chromosome elasticity to five- and six-base-specific restriction enzymes. These results, combined with previous studies of effects of nucleases on mitotic chromosome structure, indicate that mild proteolysis gradually reduces the density of chromatin-constraining elements in the mitotic chromosome, providing evidence consistent with an anisotropically folded "chromatin network" model of mitotic chromosome architecture.
authors.affiliation: Department of Physics, University of Illinois at Chicago, Chicago, IL 60607-7059, USA.
authors.forename: Lisa H
authors.initials: LH
authors.lastname: Pope
authors.forename: Chee
authors.initials: C
authors.lastname: Xiong
authors.forename: John F
authors.initials: JF
authors.lastname: Marko
mesh_terms: D000818:Animals; D016880:Anisotropy; D002460:Cell Line; D002875:Chromosomes / Q000737:chemistry* / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004548:Elasticity; D019286:Endopeptidase K / Q000378:metabolism; D008938:Mitosis; D008968:Molecular Conformation; D009673:Notophthalmus viridescens / Q000235:genetics / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: C028868:AGGCCT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C024400:GTYRAC-specific type II deoxyribonucleases; D014357:Trypsin; D019286:Endopeptidase K