[Microbial community structure analyzed by single-strand conformation polymorphism technique in sulfate-reducing reactor].
Analyses of microbial community structure and the relationships between Sulfate-Reducing Bacteria (SRBs) and Acidogenic Bacteria (ABs) in a completely stirred sulfate-reducing reactor were carried out by modified polymerase chain reaction-single-stranded conformation polymorphism (PCR-SSCP) targeted eubacterial 16S ribosomal RNA gene. A total of 13 bands were obtained and 6 of them (A1, A3, A4, A5, A9, A10) were sequenced. The sequences are similar to Leuconostoc mesenteroides (GenBank Access No. AY453065), some uncultured bacteria (AJ318147, AF227834, AJ576427), Ethanologenbacterium (AY434722), Clostridiaceae (AB084627), etc. In order to investigate the SRBs in the reactor,the active sludge was cultured on SRB-selected media and also did SSCP with the compound cultured bacteria. Two new bands appeared, one similar to Bacteroidetes (AB074606) and another similar to Desulfovibrio (Y12254, U42221). The experimental results indicate that the proportion of SRBs in the reactor is probably less than 1.5 percent. But the few SRBs play a very important role during the course of sulfate reduction via cooperation with acidogenic
Zhao
Yang-Guo
YG
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. zhaoyangguo@hit.edu.cn
Ren
Nan-Qi
NQ
Wang
Ai-Jie
AJ
Liu
Guang-Min
GM
Zhao
Qiu-Shi
QS
Shang
Huai-Xiang
HX
D000143:Acids / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology*; D015169:Colony Count, Microbial; D006863:Hydrogen-Ion Concentration; D007934:Leuconostoc / Q000235:genetics / Q000254:growth & development; D018807:Polymorphism, Single-Stranded Conformational; D012336:RNA, Ribosomal, 16S; D013431:Sulfates / Q000737:chemistry*; D016999:Sulfur-Reducing Bacteria / Q000235:genetics / Q000254:growth & development*
D000143:Acids; D012336:RNA, Ribosomal, 16S; D013431:Sulfates