Suppression of cell death in primary rat hepatocytes by alpha1-acid glycoprotein.
In screening for effective additives for the long-term culture of hepatocytes, the hepatoprotective effect of alpha1-acid glycoprotein (AGP) was observed. AGP prevented primary hepatocytes from undergoing cell death induced by the chemical toxin, bromobenzene. Moreover, AGP added to medium was found to maintain the number of viable hepatocytes for as long as 6 d. The hepatoprotective effect of AGP was lost by removing sialic acid groups at the N-glycan chain terminal of AGP. It is shown that the complete form of N-glycan chain is needed for the hepatoprotectivity of AGP.
Kagaya
Noritaka
N
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
Kamiyoshi
Akiko
A
Tagawa
Yoh-Ichi
Y
Akamatsu
Soichiro
S
Isoda
Katsuhiro
K
Kawase
Masaya
M
Yagi
Kiyohito
K
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001798:Blood Proteins / Q000494:pharmacology*; D001969:Bromobenzenes / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology; D004347:Drug Interactions; D006023:Glycoproteins / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D009961:Orosomucoid; D051381:Rats; D017207:Rats, Sprague-Dawley
D001798:Blood Proteins; D001969:Bromobenzenes; D006023:Glycoproteins; C506108:Orm1 protein, rat; D009961:Orosomucoid; C032036:bromobenzene