title: Metabolic conversion of 24-methyl-Delta25-cholesterol to 24-methylcholesterol in higher plants.
abstract: Feeding of chemically synthesized [27-13C]codisterol ([27-13C]2), [27-13C]24-epicodisterol ([27-13C]3), [23,24-2H2]codisterol ([23,24-2H2]2), and [26,27-2H6]24-methyldesmosterol ([26,27-2H6]8) to Oryza sativa cell cultures, followed by MS and NMR analysis of the biosynthesized dihydrobrassicasterol (9)/campesterol (10), revealed that both (24R)- and (24S)-epimers of 24-methyl-Delta25-cholesterol (2/3) were converted to 9 and 10 via the common intermediate 24-methyldesmosterol (8).
authors.affiliation: Kyoritsu University of Pharmacy, Shibakoen, Tokyo 105-8512, Japan. takahashi-ky@kyoritsu-ph.ac.jp
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takahashi
authors.forename: Kozue
authors.initials: K
authors.lastname: Nasu
authors.forename: Tadahiko
authors.initials: T
authors.lastname: Mashino
authors.forename: Masuo
authors.initials: M
authors.lastname: Morisaki
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Hara
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Fujimoto
mesh_terms: D002478:Cells, Cultured; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D012275:Oryza / Q000378:metabolism; D010840:Phytosterols / Q000096:biosynthesis* / Q000737:chemistry; D010944:Plants / Q000378:metabolism*
chemical_list: D010840:Phytosterols; C021273:campesterol; D002784:Cholesterol