Systematic transient assays of promoter activities for leaf-specific genes identified by gene-expression profiling with cDNA microarrays in Arabidopsis thaliana.
We identified 85 genes highly expressed in leaves using an Arabidopsis cDNA microarray. A vector, pRAB5, was designed to allow cloning and assaying of the promoters. Fifty-one promoters from the selected genes were cloned and then assayed using a microprojectile bombardment and dual luciferase reporter assay system. This system allowed efficient systematic assays of promoter activity.
Yamakawa
Seiyei
S
Technology Department Research Association for Biotechnology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan. s-yamaka@bs.naist.jp
Ando
Kohei
K
Chisaka
Aya
A
Yoshida
Kazuya
K
Shinmyo
Atsuhiko
A
Kohchi
Takayuki
T