Detection of 2,4-dichlorophenoxyacetic acid in rat milk of dams exposed during lactation and milk analysis of their major components.
2,4-Dichlorophenoxyacetic acid (2,4-D) and its derivatives are herbicides widely used to control the growth of broadleaf and woody plant. Human and animal exposure to 2,4-D through agriculture use, food products, or use in lawn and garden care has been well documented, but little information is available on the transfer from serum to milk in exposed dams. In this study, we measured the content of 2,4-D in rat milk from mother exposed to 15, 25, 50 or 7 0mg 2,4-D/kg bw through the diet (4 treated groups, 8 dam each; 1 control group with 8 dams) over a period of 16 days starting on the post-natal day 1 (PND 1). The effect of 2,4-D on milk components was also evaluated. All doses tested caused a decrease in the body weight gain of the pups (4 groups, 64 pups each). It also produce a 30% in the content of total lipids and a changed the content of minor proteins in milk of the treated groups. 2,4-D produces an important decrease in some fatty acids content, being the polyunsaturated fatty acids the most affected. Further analysis showed that 2,4-D concentrations chromatographically detected both serum of dams and pups and milk were dose-dependent.
Stürtz
N
N
Experimental Toxicology Laboratory, Department of Physiological Sciences, School of Biochemical and Pharmaceutical Sciences, National University of Rosario, Argentina.
Bongiovanni
B
B
Rassetto
M
M
Ferri
A
A
de Duffard
A M Evangelista
AM
Duffard
R
R
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis / Q000493:pharmacokinetics*; D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D001835:Body Weight / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006540:Herbicides / Q000032:analysis / Q000493:pharmacokinetics*; D007774:Lactation / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015430:Weight Gain / Q000187:drug effects
D005227:Fatty Acids; D006540:Herbicides; D008894:Milk Proteins; D015084:2,4-Dichlorophenoxyacetic Acid