Evolution of a rare vaccine-derived multirecombinant poliovirus.
Recombination is one of the mechanisms by which viral genomes evolve. A vaccine-derived multirecombinant poliovirus strain was isolated from a 5-month-old child with vaccine-associated paralytic poliomyelitis after oral poliovirus vaccine administration. The isolate had an S2/S1/S2/S1 primary genomic structure as revealed by restriction fragment length polymorphism and sequencing analysis. Recombination of the middle S1/S2 region is extremely rare and one of the few characterized types of recombination with Sabin type 1 as a 5' partner. An attempt was made to perform evolutionary analysis of the contributing sequences using the identified mutations in comparison with the original Sabin sequences. A hypothesis is proposed for the order in which the identified recombination events occurred.
Karakasiliotis
Ioannis
I
Department of Virology, Hellenic Pasteur Institute, 127 Vas. Sofias Avenue, 11521 Athens, Greece.
Paximadi
Eleni
E
Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos and Aiolou Street, 41221 Larissa, Greece.
Markoulatos
Panayotis
P
Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos and Aiolou Street, 41221 Larissa, Greece.
D019143:Evolution, Molecular; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D011051:Poliomyelitis / Q000209:etiology / Q000517:prevention & control / Q000821:virology*; D017955:Poliovirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D011055:Poliovirus Vaccine, Oral / Q000009:adverse effects / Q000235:genetics*; D011995:Recombination, Genetic
D011055:Poliovirus Vaccine, Oral