Effects of hypertonic (7%) saline on brain injury in experimental Escherichia coli meningitis.
We sought to know whether hypertonic (7%) saline (HTS) attenuates brain injury by improving cerebral perfusion pressure (CPP) and down-modulating acute inflammatory responses in experimental bacterial meningitis in the newborn piglet. Twenty-five newborn piglets were assorted into three groups: 6 in the control group (C), 10 in the meningitis group (M), and 9 in the meningitis with HTS infusion group (H). Meningitis was induced by intracisternal injection of 10(8) colony forming units of Escherichia coli in 100 microL of saline. 10 mL/kg of HTS was given intravenously as a bolus 6 hr after induction of meningitis, thereafter the infusion rate was adjusted to maintain the serum sodium level between 150 and 160 mEq/L. HTS significantly attenuated meningitis-induced brain cell membrane disintegration and dysfunction, as indicated by increased lipid peroxidation products and decreased Na+, K+-ATPase activity in the cerebral cortex in M. HTS significantly attenuated acute inflammatory markers such as increased intracranial pressure, elevated lactate level and pleocytosis in the cerebrospinal fluid observed in M. Reduced CPP observed in M was also significantly improved with HTS infusion. These findings implicate some attenuation of the meningitis-induced alterations in cerebral cortical cell membrane structure and function with HTS, possibly by improving CPP and attenuating acute inflammatory responses.
Choi
Chang Won
CW
Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Hwang
Jong Hee
JH
Chang
Yun Sil
YS
Park
Won Soon
WS
Kim
Beyong Il
BI
Choi
Jung-Hwan
JH
Lee
Munhyang
M
D000818:Animals; D000831:Animals, Newborn; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D001927:Brain Diseases / Q000188:drug therapy* / Q000473:pathology*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000473:pathology*; D004195:Disease Models, Animal; D007427:Intracranial Pressure / Q000187:drug effects; D020814:Meningitis, Escherichia coli / Q000150:complications / Q000188:drug therapy* / Q000473:pathology*; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage*; D013552:Swine; D016896:Treatment Outcome
D000893:Anti-Inflammatory Agents; D012462:Saline Solution, Hypertonic