Modulation of Fas-induced apoptosis by p75 neurotrophin receptor in a human neuroblastoma cell line.
Fas and p75 neurotrophin receptors (p75(NTR)) are death receptors that alone induce apoptosis of SH-SY5Y neuroblastoma cell line respectively by Fas ligand or brain-derived neurotrophic factor (BDNF, a p75(NTR) ligand). We report on the modulation of Fas-mediated apoptosis by concomitant p75(NTR) activation. The exposure to both ligands suppressed the apoptotic effect. A co-localisation of Fas and p75(NTR) receptors was evidenced by co-capping and immunoprecipitation assays. Moreover, a caspase-8 inhibitor suppressed the protective effect of the concomitant BDNF and Fas ligand stimulation, suggesting that p75(NTR) and Fas receptors could share common signalling pathways.
Giraud
S
S
Laboratory of Immunology, EA 3842, University Hospital, 87042 Limoges, France.
Lautrette
C
C
Bessette
B
B
Decourt
C
C
Mathonnet
M
M
Jauberteau
M-O
MO
D017209:Apoptosis / Q000187:drug effects; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D061945:Caspase Inhibitors; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000494:pharmacology; D053222:Fas Ligand Protein / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008954:Models, Biological; D009447:Neuroblastoma / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020800:Receptor, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D020813:Receptor, trkB / Q000378:metabolism; D013108:Sphingomyelin Phosphodiesterase / Q000037:antagonists & inhibitors; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D061945:Caspase Inhibitors; D004791:Enzyme Inhibitors; C491901:FAS protein, human; D053222:Fas Ligand Protein; D012333:RNA, Messenger; D020800:Receptor, Nerve Growth Factor; D019014:fas Receptor; D020813:Receptor, trkB; D013108:Sphingomyelin Phosphodiesterase; C519633:SMPD2 protein, human