title: Construction of recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for ferulic acid biotransformation to vanillin.
abstract: Recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 capable of ferulic acid biotransformation to vanillin were constructed using homologous recombination and a PCR based cloning strategy, respectively. In the liquid culture of P. putida BO14, 26.81+/-2.30 microg vanillin ml(-1) of culture filtrate was detected. In the case of recombinant E. coli QEFCA8, 19.37+/-1.95 microg vanillin ml(-1) of culture filtrate was detected. Results indicate that the strains could be useful for the biotechnological production of vanillin, a very important flavoring substance.
authors.affiliation: Bacteriology Unit, International Centre for Genetic Engineering and Biotechnology, Area Science Park, Padriciano 99, Trieste 34012, Italy.
authors.forename: B C
authors.initials: BC
authors.lastname: Okeke
authors.forename: V
authors.initials: V
authors.lastname: Venturi