title: Enhancement of isoflavone synthase activity by co-expression of P450 reductase from rice.
abstract: Plant cytochrome P450s interact with a flavoprotein, NADPH-cytochrome P450 reductase (CPR), to transfer electrons from NADPH. The gene for rice P450 reductase (RCPR) was cloned and expressed in Saccaromyces cerevisiae, where the specific activity of the expressed RPCR was 0.91 U/mg protein. When isoflavone synthase gene (IFS) from red clover, used as a model system of plant cytochrome P450, was co-expressed with RCPR in yeast, the production of genistein from naringein increased about 4.3-fold, indicating that the RCPR efficiently interacts with cytochrome P450 to transfer electrons from NADPH.
authors.affiliation: Bio/Molecular Informatics Center, Department of Molecular Biotechnology, Konkuk University, 143-701, Seoul, Korea.
authors.forename: Dae Hwan
authors.initials: DH
authors.lastname: Kim
authors.forename: Bong Gyu
authors.initials: BG
authors.lastname: Kim
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Yoongho
authors.initials: Y
authors.lastname: Lim
authors.forename: Hor Gil
authors.initials: HG
authors.lastname: Hur
authors.forename: Joong-Hoon
authors.initials: JH
authors.lastname: Ahn
mesh_terms: D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D019833:Genistein / Q000378:metabolism; D029902:Medicago / Q000201:enzymology* / Q000235:genetics; D009251:NADPH-Ferrihemoprotein Reductase / Q000096:biosynthesis* / Q000235:genetics; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D010105:Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System; D019833:Genistein; D010105:Oxygenases; C048489:isoflavone synthase; D009251:NADPH-Ferrihemoprotein Reductase