title: Proteomic identification of activin receptor-like kinase-1 as a differentially expressed protein during hyaloid vascular system regression.
abstract: The hyaloid vascular system (HVS) is a transient network of capillaries that nourishes the embryonic lens and the primary vitreous of the developing eye. We used proteomic analysis and immunohistochemical staining to identify activin receptor-like kinase-1 (ALK1), a type I receptor for transforming growth factor-beta1, during the HVS regression phase. In addition, we overexpressed ALK1 in corneas implanted with bFGF (basic fibroblast growth factor) pellets and observed that ALK1 overexpression resulted in inhibition of bFGF-induced corneal neovascularization in vivo. Our data suggest that ALK1 may play a role in HVS regression during ocular development.
authors.affiliation: Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
authors.forename: Elena
authors.initials: E
authors.lastname: Albè
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Escalona
authors.forename: Rama
authors.initials: R
authors.lastname: Rajagopal
authors.forename: Joel A
authors.initials: JA
authors.lastname: Javier
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Chang
authors.forename: Dimitri T
authors.initials: DT
authors.lastname: Azar
mesh_terms: D030201:Activin Receptors, Type I / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D030301:Activin Receptors, Type II; D000818:Animals; D001158:Arteries / Q000196:embryology; D003315:Cornea / Q000098:blood supply / Q000196:embryology; D004247:DNA / Q000235:genetics; D042783:Endothelial Cells / Q000737:chemistry; D005123:Eye / Q000098:blood supply* / Q000196:embryology*; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D007908:Lens, Crystalline / Q000196:embryology / Q000378:metabolism; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000235:genetics; D040901:Proteomics; D017539:Tunica Intima / Q000166:cytology; D014822:Vitreous Body / Q000196:embryology / Q000378:metabolism
chemical_list: D005346:Fibroblast Growth Factors; D004247:DNA; D030201:Activin Receptors, Type I; D030301:Activin Receptors, Type II; C498931:Acvrl1 protein, mouse