Characterization of genomic instability in ulcerative colitis neoplasia leads to discovery of putative tumor suppressor regions.
Ulcerative colitis (UC) is an inflammatory disease of the colon that is associated with increased risk of colorectal cancer associated with genomic instability. We have previously demonstrated that genomic instability is present in UC patients with colonic neoplasia, and hypothesized that the chromosomal alterations may be taking place in regions that are susceptible to mutation or that provide a growth advantage to a cell undergoing neoplastic transformation. In this study, we used two polymerase chain reaction (PCR)-based DNA fingerprinting techniques (arbitrarily primed PCR and inter-simple-sequence-repeat PCR) to study the process of genomic instability. The two techniques of DNA fingerprinting cross-validate the instability observed in these studies. We analyzed the molecular basis of 10 commonly altered DNA bands obtained from DNA fingerprints of biopsies from various histologic grades of UC patients with dysplasia or cancer (UC Progressors). We determined that the band changes in the fingerprint truly represent changes in DNA sequence, and that the fingerprinting provides highly reproducible results. Furthermore, our investigation revealed that 40% of alterations involve repetitive sequences. Two frequently deleted sequences in 6q27 and 2q14 were studied further because they were frequently abnormal in the dysplastic and nondysplastic tissue of UC Progressors. The losses from 6q27 and 2q14 were confirmed by loss of heterozygosity and real-time PCR analysis. Both of these regions in chromosomes 6 and 2 are surrounded by highly repetitive and mobile LINE-1 elements, possibly making the region susceptible to mutational change. These regions were affected (lost) in UC Progressors but not in UC patients who were neoplasia free. Loss of heterozygosity at 6q27 has been described in ovarian and other cancers, while the 2q14 region has been implicated in prostate and sporadic colon cancers. Both regions are likely to contain tumor-suppressor genes. In conclusion, the genomic instability in UC Progressors can occur in regions that are susceptible to change and are locations of putative tumor-suppressor genes.
Chen
Ru
R
Division of Gastroenterology, Department of Pathology, University of Washington, Seattle 98195, USA.
Bronner
Mary P
MP
Crispin
David A
DA
Rabinovitch
Peter S
PS
Brentnall
Teresa A
TA
D003093:Colitis, Ulcerative / Q000150:complications* / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics*; D016172:DNA Fingerprinting; D016147:Genes, Tumor Suppressor; D042822:Genomic Instability; D006801:Humans; D019656:Loss of Heterozygosity; D009154:Mutation; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid