Evaluation of cell membrane integrity of spermatozoa by hypoosmotic swelling test - "water test" in mice after intraperitoneal daidzein administration.
The functional integrity of the cell membrane of the mice sperm after daidzein administration intraperitoneal was assessed by studying the swelling reaction of the sperm when suspended in distilled water. The results obtained in water test indicate that daidzein, one of the phytoestrogens, decreases the number of normal and live sperm (swollen) in the samples obtaining from vas deferens. This effect was statistically significant in the mice after 7 and 14 days of daidzein administration. In mice treated with daidzein the number of dead (unswollen) sperm was increased, especially in animals after 14 days of experiment.
Sliwa
L
L
Department of Human Developmental Biology, Collegium Medicum, Jagiellonian University, Kraków, Poland. leosliwa@cm-uj.krakow.pl
Macura
B
B
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology; D007274:Injections, Intraperitoneal; D007529:Isoflavones / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009994:Osmolar Concentration; D048789:Phytoestrogens / Q000008:administration & dosage / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D014867:Water
D007529:Isoflavones; D048789:Phytoestrogens; D014867:Water; C004742:daidzein