Expression of macrophage-selective markers in human and rodent adipocytes.
CD14, CD68 and/or mouse F4/80 or human epidermal growth factor module-containing mucin-like receptor 1 (EMR1) are widely used as macrophage-specific markers. Since macrophages infiltrate several tissues during inflammatory processes, CD14, CD68 and EMR1-F4/80 have been employed to discriminate between tissue-containing macrophages, like adipose tissue (AT), and other cells. Using real-time PCR experiments, we show that isolated adipocytes from humans and mice AT express high levels of CD14 and CD68 mRNA, whereas EMR1-F4/80 is mainly present in the macrophage-containing stroma-vascular fraction. Furthermore, fibroblasts-like cells (adipoblasts), preadipocytes and adipocytes from the murine cell lines, 3T3-F442A and BFC-1, express CD14 and CD68 mRNA and protein as determined by fluorescence-activated cell sorter, but not F4/80 which, as expected, is strongly expressed in the macrophage cell line RAW264.7. These results reinforce the view that EMR1-F4/80 is the best macrophage marker to date and show that CD14 and CD68 are not macrophage-specific proteins.
Khazen
Wael
W
Institut National de la Santé et de la Recherche Médicale, Unit 530, Université Paris Descartes, Centre Universitaire des Saints-Pères, France.
M'bika
Jean-Pierre
JP
Tomkiewicz
Céline
C
Benelli
Chantal
C
Chany
Charles
C
Achour
Ammar
A
Forest
Claude
C
D017667:Adipocytes / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism*; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics; D015415:Biomarkers / Q000032:analysis; D002135:Calcium-Binding Proteins; D015870:Gene Expression; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009077:Mucins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D000097382:CD68 Molecule
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D002135:Calcium-Binding Proteins; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D009077:Mucins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C094471:ADGRE1 protein, human; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C036763:monocyte-macrophage differentiation antigen