title: Functional proteomics and correlated signaling pathway of the thermophilic bacterium Bacillus stearothermophilus TLS33 under cold-shock stress.
abstract: The thermophilic bacterium Bacillus stearothermophilus TLS33 was examined under cold-shock stress by a proteomic approach to gain a better understanding of the protein synthesis and complex regulatory pathways of bacterial adaptation. After downshift in the temperature from 65 degrees C, the optimal growth temperature for this bacterium, to 37 degrees C and 25 degrees C for 2 h, we used the high-throughput techniques of proteomic analysis combining 2-DE and MS to identify 53 individual proteins including differentially expressed proteins. The bioinformatics database was used to search the biological functions of proteins and correlate these with gene homology and metabolic pathways in cell protection and adaptation. Eight cold-shock-induced proteins were shown to have markedly different protein expression: glucosyltransferase, anti-sigma B (sigma(B)) factor, Mrp protein homolog, dihydroorthase, hypothetical transcriptional regulator in FeuA-SigW intergenic region, RibT protein, phosphoadenosine phosphosulfate reductase and prespore-specific transcriptional activator RsfA. Interestingly, six of these cold-shock-induced proteins are correlated with the signal transduction pathway of bacterial sporulation. This study aims to provide a better understanding of the functional adaptation of this bacterium to environmental cold-shock stress.
authors.affiliation: Institute of Biological Chemistry and Genomics Research Center, Academia Sinica, Taipei, Taiwan.
authors.forename: Supachai
authors.initials: S
authors.lastname: Topanurak
authors.forename: Supachok
authors.initials: S
authors.lastname: Sinchaikul
authors.forename: Boonyaras
authors.initials: B
authors.lastname: Sookkheo
authors.forename: Suree
authors.initials: S
authors.lastname: Phutrakul
authors.forename: Shui-Tein
authors.initials: ST
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification; D001411:Geobacillus stearothermophilus / Q000737:chemistry / Q000254:growth & development*; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D004798:Enzymes; D010446:Peptide Fragments; D020543:Proteome