Profiling of differentially expressed genes in LRRC4 overexpressed glioblastoma cells by cDNA array.
Our previous study has shown that LRRC4 is a novel member of the leucine-rich repeat (LRR) superfamily and has the potential to suppress brain tumor growth. In order to further analyze the functions of LRRC4 on the maintenance of normal function and suppression of tumorigenesis in the central nervous system, we investigated alterations in gene expression related to neurobiology by the Atlas array in two inducible dual-stable LRRC4-overexpressing cell lines. Seventeen of 588 genes spotted on the Atlas membrane showed altered expression levels in LRRC4 transfected U251MG Tet-on cells, which are involved in cell proliferation and cell cycle progression, tumor invasion and metastasis, and neurotransmitter synthesis and release. In addition, cell invasion assay results showed that LRRC4 can inhibit the U251MG cell migration. These studies represent the first cDNA array analysis of the effects of LRRC4 on the involvement of different neurobiological genes in U251MG glioblastoma cells and provide new insights into the function of LRRC4 in glioma.
Zhang
Qiu-Hong
QH
Cancer Research Institute, Central South University, Changsha 410078, China.
Wu
Ming-Hua
MH
Wang
Li-Li
LL
Cao
Li
L
Tang
Ke
K
Peng
Cong
C
Gan
Kai
K
Li
Xiao-Ling
XL
Li
Gui-Yuan
GY
D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D020869:Gene Expression Profiling / Q000379:methods; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation; D020691:rab GTP-Binding Proteins / Q000096:biosynthesis
D015815:Cell Adhesion Molecules; C503331:LRRC4 protein, human; D009419:Nerve Tissue Proteins; D020691:rab GTP-Binding Proteins