The levels of plasma IL-1beta, IL-6 of C57BL/6J mice treated with MPTP and brain lateralization.
The work is to explore the relationship between the levels of cytokines (IL-1beta and IL-6) in C57BL/6J mice treated with MPTP and brain lateralization. By using paw preference test, right-pawed, left-pawed mice models were established. Following single injection of 1-methyl-4-pheny1-2, 3, 6-tetrahydropyrid (MPTP) (40 mg/kg) to impair dopaminergic neuron, enzyme linked immunosorbent assay (ELISA) kits were used for detection of plasma levels of cytokines. The results showed that in saline treated C57BL/6J mice (control), there was no obvious difference observed between left-pawed and right-pawed mice in plasma levels of IL-1beta and IL-6. In MPTP treated mice, there was no difference between level of IL-1beta in left-pawed mice and that in right-pawed ones in statistics, that is, they were increased on day 1 and day 3, but decreased on day 6. The plasma level of IL-6 was lower in left-pawed than that in right-pawed mice (p < 0.005) after MPTP treatment. On day 1 and day 3, the level of IL-6 was almost the same as control; on day 6, it was significantly increased, higher than that of control (p < 0.001) in left-pawed mice. While in right-pawed mice, on day 1 and day 3, it was no different from control, too. And on day 6, it significantly increased in compared with control (p < 0.005). In conclusion, the level of plasma IL-6 of C57BL/6J mice treated with MPTP increased. The variation of IL-6 was correlated to brain lateralization.
Luo
Yanling
Y
Department of Microbiology and Immunology, Shantou University Medical College, Shantou 515031, Guangdong, China.
Su
Yun
Y
Shen
Yanqin
Y
Zhao
Lichun
L
Li
Kangsheng
K
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000008:administration & dosage*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D015259:Dopamine Agents / Q000008:administration & dosage*; D007839:Functional Laterality / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D008297:Male; D051379:Mice
D015259:Dopamine Agents; D007375:Interleukin-1; D015850:Interleukin-6; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine