title: Identification of mutation sites on delta5 desaturase genes from Mortierella alpina 1S-4 mutants.
abstract: The mutation sites on delta5 desaturase genes in delta5 desaturase-defective mutants derived from arachidonic acid-producing Mortierella alpina 1S-4 were identified. The mutations resulted in an amino acid replacement (G189E or W301Stop) and uncorrected transcription caused by recognition of an AG-terminal newly created on C207A gene mutation, resulting in low or no delta5 desaturase activity in these mutants.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Abe
authors.forename: Eiji
authors.initials: E
authors.lastname: Sakuradani
authors.forename: Takuji
authors.initials: T
authors.lastname: Ueda
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D004252:DNA Mutational Analysis / Q000379:methods*; D000090943:Delta-5 Fatty Acid Desaturase; D044943:Fatty Acid Desaturases / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D020094:Mortierella / Q000201:enzymology* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
chemical_list: D000090943:Delta-5 Fatty Acid Desaturase; D044943:Fatty Acid Desaturases