Production of alkaline protease with Teredinobacter turnirae in controlled fed-batch fermentation.
By using our previously optimized media and a fed-batch operation controlled by LabVIEW Software, the key parameter for a high production of alkaline protease using the marine bacterium, Teredinobacter turnirae, was to maintain a low concentration of C and N-sources ( < 2 g sucrose l(-1) and < 0.2 g NH4C l l(-1)) using an appropriate fed-batch culture system. A maximum protease activity of 8250 U ml(-1 )was thus achieved.
Beshay
Usama
U
Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research and Technology Applications, New Bourg El-Arab City, Research Area, 21934, Alexandria, Egypt. ugabra10@hotmail.com
Moreira
Antonio
A
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D049872:Bivalvia / Q000382:microbiology; D010450:Endopeptidases / Q000096:biosynthesis*; D005285:Fermentation
D001426:Bacterial Proteins; D010450:Endopeptidases; C087822:alkaline protease