title: Three biflavonoids from ethanol extract of the root of Daphne genkwa.
abstract: OBJECTIVE
  To investigate the chemical constituents of the secondary metabolites of the roots of Daphne genkwa.
  
  
  METHODS
  The roots of D. genkwa were extracted with 95% ethanol at 60-70 degrees C for 7 days to obtain the crude extract. The crude extract was purified by silica gel and Sephadex LH-20 column chromatography as well as the HPLC techniques. The structures of the isolates were elucidated by combined spectroscopic methods including 1D and 2D NMR, MS, UV, IR and CD.
  
  
  RESULTS
  Three new biflavonoids were isolated from the ethanol extract of the roots of D. genkwa and their structures were identified as daphnodorin H-3-methyl ether (1), daphnodorin H-3"-methyl ether (2) and daphnodorin G-3"-methyl ether (3).
  
  
  CONCLUSIONS
  Compounds 1, 2 and 3 are three new biflavonoids.
authors.affiliation: Key Laboratory for Biotechnology of Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Jiangsu 221116, China. yyzw@xznu.edu.cn
authors.forename: Wei-fa
authors.initials: WF
authors.lastname: Zheng
authors.forename: Feng
authors.initials: F
authors.lastname: Shi
mesh_terms: D044946:Biflavonoids / Q000737:chemistry / Q000302:isolation & purification*; D032364:Daphne / Q000737:chemistry*; D000431:Ethanol; D008968:Molecular Conformation; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D044946:Biflavonoids; C514833:daphnodorin G-3''-methyl ether; C514832:daphnodorin H-3''-methyl ether; C514831:daphnodorin H-3-methyl ether; D000431:Ethanol