title: Synthesis and characterization of DNA quadruplexes containing T-tetrads formed by bunch-oligonucleotides.
abstract: The solid phase syntheses of the bunch oligonucleotides and based on the sequences of the natural oligodeoxynucleotides (ODNs) d(TG2TG2C) and d(CG2TG2T), respectively, attached to a non-nucleotidic tetrabranched linker, are reported. Bunch-ODNs and were shown to form more stable monomolecular parallel G-quadruplexes and when compared with their tetramolecular counterparts [d(TG2TG2C)]4 and [d(CG2TG2T)]4, respectively. The structure and stability of all the synthesized complexes have been investigated by circular dichroism (CD), CD thermal denaturation experiments, and 1H-NMR (nuclear magnetic resonance) experiments at variable temperatures. Particularly, the spectroscopic data confirmed that 1 adopts a T-tetrad containing parallel-stranded quadruplex structure as in the tetramolecular complex.
authors.affiliation: Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli Federico II, Via D. Montesano 49, I-80131 Napoli, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Oliviero
authors.forename: Jussara
authors.initials: J
authors.lastname: Amato
authors.forename: Nicola
authors.initials: N
authors.lastname: Borbone
authors.forename: Aldo
authors.initials: A
authors.lastname: Galeone
authors.forename: Michela
authors.initials: M
authors.lastname: Varra
authors.forename: Gennaro
authors.initials: G
authors.lastname: Piccialli
authors.forename: Luciano
authors.initials: L
authors.lastname: Mayol
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry*; D054856:G-Quadruplexes; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D004247:DNA