Compact oleic acid in HAMLET.
HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between alpha-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.
Fast
Jonas
J
Department of Biophysical Chemistry, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. jfast@colorado.edu
Mossberg
Ann-Kristin
AK
Nilsson
Hanna
H
Svanborg
Catharina
C
Akke
Mikael
M
Linse
Sara
S
D006801:Humans; D007768:Lactalbumin / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D019301:Oleic Acid / Q000737:chemistry*; D009829:Oleic Acids / Q000737:chemistry*; D011487:Protein Conformation; D017510:Protein Folding
C506422:HAMLET complex, human; D009829:Oleic Acids; D019301:Oleic Acid; D007768:Lactalbumin