Effects of ferulic acid on E-selectin expression in activated endothelial cell and leukocyte-endothelial cell adhesion.
OBJECTIVE
To study the effects of ferulic acid (FA) on E-selectin expression in human umbilical vein endothelial cells (HUVECs) activated by lipopolysaccharide and leukocyte-endothelial cell adhesion.


METHODS
The effects of FA on E-selectin and E-selectin mRNA expression were determined by flow cytometry and reverse transcription polymerase chain reaction. The effect of FA on HL60-HUVEC adhesion was evaluated with the method of staining the cells by Rose Bengal.


RESULTS
The expression of E-selectin and E-selectin mRNA were down regulated by FA (0.62 and 0.41 mmol x L(-1), respectively). HL60 cells adhered to activated HUVECs were also reduced by FA (0.62 and 0.41 mmol x L(-1), respectively).


CONCLUSIONS
FA can inhibit the expression of E-selectin and E-selectin mRNA and HL60-HUVEC adhesion. This may contribute to its protective effect against ischemia-reperfusion injury.
Wang
Xiao-lan
XL
First Affiliated Hospital of Suzhou University, Jiangsu Institute of Hematology, Suzhou 215006, China.
Hu
Xiao-hui
XH
Lü
Ming-en
ME
Gu
Zhen-lun
ZL
Ruan
Chang-geng
CG
D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D003373:Coumaric Acids / Q000494:pharmacology*; D019040:E-Selectin / Q000096:biosynthesis* / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism*; D018922:HL-60 Cells / Q000502:physiology; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology
D003373:Coumaric Acids; D019040:E-Selectin; D012333:RNA, Messenger; C004999:ferulic acid