title: The electrocardiogram of human embryonic stem cell-derived cardiomyocytes.
abstract: Microelectrode arrays enable to map extracellular field potentials of excitable organs (eg, cardiac tissue) in an electrocardiogram-like manner: They allow to detect (a) rhythmicity, (b) the origin and route of excitation, (c) repolarization, and (d) conduction in heart tissue in short- and long-term experimental approaches. Using it as a screening tool for potential side effects of drugs, we here provide evidence for d-sotalol-induced delayed repolarization in human embryonic stem (hES) cell-derived cardiomyocytes. Thus, the combination of the microelectrode array system with cardiac clusters derived from hES cells heralds a paradigm shift toward improved pharmaceutical drug safety. However, the mixture of various cell types in hES cell cardiac clusters (eg, atrial, immature and mature ventricular cardiomyocytes) indicates the strong need for improved selectivity of cardiac differentiation protocols using hES cells.
authors.affiliation: Institute of Neurophysiology, University of Cologne, D-50931 Cologne, Germany. akp72@uni-koeln.de
authors.forename: Michael
authors.initials: M
authors.lastname: Reppel
authors.forename: Frank
authors.initials: F
authors.lastname: Pillekamp
authors.forename: Konrad
authors.initials: K
authors.lastname: Brockmeier
authors.forename: Matthias
authors.initials: M
authors.lastname: Matzkies
authors.forename: Aret
authors.initials: A
authors.lastname: Bekcioglu
authors.forename: Teresa
authors.initials: T
authors.lastname: Lipke
authors.forename: Filomain
authors.initials: F
authors.lastname: Nguemo
authors.forename: Hendrik
authors.initials: H
authors.lastname: Bonnemeier
authors.forename: Juergen
authors.initials: J
authors.lastname: Hescheler
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D004562:Electrocardiography; D022062:Electrophysiologic Techniques, Cardiac; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects; D006329:Heart Conduction System / Q000187:drug effects / Q000196:embryology; D006801:Humans; D008839:Microelectrodes; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000502:physiology*; D013015:Sotalol / Q000494:pharmacology; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology*
chemical_list: D000319:Adrenergic beta-Antagonists; D013015:Sotalol