title: Protein production and crystallization at SECSG -- an overview.
abstract: Using a high degree of automation, the Southeast Collaboratory for Structural Genomics (SECSG) has developed high throughput pipelines for protein production, and crystallization using a two-tiered approach. Primary, or tier-1, protein production focuses on producing proteins for members of large Pfam families that lack a representative structure in the Protein Data Bank. Target genomes are Pyrococcus furiosus and Caenorhabditis elegans. Selected human proteins are also under study. Tier-2 protein production, or target rescue, focuses on those tier-1 proteins, which either fail to crystallize or give poorly diffracting crystals. This two tier approach is more efficient since it allows the primary protein production groups to focus on the production of new targets while the tier-2 efforts focus on providing additional sample for further studies and modified protein for structure determination. Both efforts feed the SECSG high throughput crystallization pipeline, which is capable of screening over 40 proteins per week. Details of the various pipelines in use by the SECSG for protein production and crystallization, as well as some examples of target rescue are described.
authors.affiliation: Southeast Collaboratory for Structural Genomics, University of Georgia, Athens, GA 30602, USA.
authors.forename: Bi-Cheng
authors.initials: BC
authors.lastname: Wang
authors.forename: Michael W W
authors.initials: MW
authors.lastname: Adams
authors.forename: Harry
authors.initials: H
authors.lastname: Dailey
authors.forename: Larry
authors.initials: L
authors.lastname: DeLucas
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
authors.forename: John
authors.initials: J
authors.lastname: Rose
authors.forename: Robert
authors.initials: R
authors.lastname: Bunzel
authors.forename: Tamara
authors.initials: T
authors.lastname: Dailey
authors.forename: Jeff
authors.initials: J
authors.lastname: Habel
authors.forename: Peter
authors.initials: P
authors.lastname: Horanyi
authors.forename: Francis E
authors.initials: FE
authors.lastname: Jenney
authors.forename: Irina
authors.initials: I
authors.lastname: Kataeva
authors.forename: Han-Seung
authors.initials: HS
authors.lastname: Lee
authors.forename: Songlin
authors.initials: S
authors.lastname: Li
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Dawei
authors.initials: D
authors.lastname: Lin
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Chi-Hao
authors.initials: CH
authors.lastname: Luan
authors.forename: Michael
authors.initials: M
authors.lastname: Mayer
authors.forename: Lisa
authors.initials: L
authors.lastname: Nagy
authors.forename: M Gary
authors.initials: MG
authors.lastname: Newton
authors.forename: Joseph
authors.initials: J
authors.lastname: Ng
authors.forename: Farris L
authors.initials: FL
authors.lastname: Poole
authors.forename: Ashit
authors.initials: A
authors.lastname: Shah
authors.forename: Claudia
authors.initials: C
authors.lastname: Shah
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Sugar
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D030562:Databases, Protein; D006801:Humans; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D040901:Proteomics / Q000295:instrumentation / Q000379:methods* / Q000458:organization & administration; D020027:Pyrococcus furiosus / Q000737:chemistry*
chemical_list: D011506:Proteins