Designing a molecularly imprinted polymer as an artificial receptor for the specific recognition of creatinine in serums.
In this study, molecularly imprinted polymers (MIP) synthesized from two different functional monomers, beta-cyclodextrin (beta-CD) and 4-vinylpyridine (4-Vpy), were prepared. The crosslinkers used for these two monomers were epichlorohydrin (EPI) and divinylbenzene (DVB), respectively. It was attempted to adsorb the target molecule, creatinine, from its mixture solutions. A proper molar ratio of monomer/crosslinker for the preparation of the imprinted poly(beta-CD) was 1:10. Between both polymers mentioned above, the affinity of the imprinted poly(4-Vpy-co-DVB) towards creatinine was comparably superior. The imprinted poly(4-Vpy-co-DVB) for creatinine could reach a specific binding ratio of 3.11. The imprinted poly(4-Vpy-co-DVB) was further utilized to bind creatinine from human serum samples. The binding capacity of the imprinted poly(4-Vpy-co-DVB) for creatinine from the serum samples was plotted against the creatinine concentration. From the correlation, the feasibility of the imprinted poly(4-Vpy-co-DVB) thus prepared for the target analyte, creatinine, was experimentally confirmed.
Hsieh
Ryh-Yaw
RY
Division of Nephrology, Yuan General Hospital, Kaohsiung, Taiwan.
Tsai
Hsuan-Ang
HA
Syu
Mei-Jywan
MJ
D003404:Creatinine / Q000097:blood*; D003432:Cross-Linking Reagents / Q000737:chemistry; D006801:Humans; D011108:Polymers / Q000737:chemistry*; D011725:Pyridines / Q000138:chemical synthesis / Q000737:chemistry*; D014753:Vinyl Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D003432:Cross-Linking Reagents; D011108:Polymers; D011725:Pyridines; D014753:Vinyl Compounds; D047392:beta-Cyclodextrins; C509367:poly(4-vinylpyridine-co-divinylbenzene); D003404:Creatinine