[Study on the interaction of water-soluble p-(N,N-dimethylaminomethyl) calix[8] arene with DNA by fluorescent spectrometry].
The interaction of water-soluble p-(N, N-dimethylaminomethyl) calix [8] arene (CX8) with DNA was studied using adriamycin(ADM) as a probe by fluorescent spectrometry. The effect factors such as, pH of solution, salt effect and unlinking DNA on the spectra were also investigated. The interaction mechanism was proposed. The authors observed that the fluorescence of ADM was quenched by calf thymus DNA, and the fluorescence intensity of DNA-ADM enhanced with the increasing of the CX8 gradually. The result indicates that the strong electrostatic interaction between the oxyphosphate anion of DNA and the CX8 existed. According to the plot of scatchard, the results imply that the influence of CX8 on interaction of DNA-ADM belongs to mixed model, on the one hand, in the condition of neutral and acidity, the oxyphosphate anion of DNA can be neutralized by CX8 partially, which induced the contraction of DNA and the conformational change of DNA, which led to release ADM from DNA partially, on the other hand, the CX8 can also compete the electrostatic sites with ADM directly.
Li
Lai-sheng
LS
The Center of analysis and Testing, Nanchang University, Nanchang, China.
Huang
Zhi-bing
ZB
Wang
Yu-xiao
YX
Liu
Xu
X
Li
Yan-ping
YP
Ge
Xiao-hui
XH
D000465:Algorithms; D000818:Animals; D047250:Calixarenes / Q000737:chemistry*; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
D005456:Fluorescent Dyes; D014867:Water; D047250:Calixarenes; D004317:Doxorubicin; D004247:DNA; C427203:calf thymus DNA