title: Detecting single stranded DNA with a solid state nanopore.
abstract: Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than approximately 11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.
authors.affiliation: Department of Physics, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Fologea
authors.forename: Marc
authors.initials: M
authors.lastname: Gershow
authors.forename: Bradley
authors.initials: B
authors.lastname: Ledden
authors.forename: David S
authors.initials: DS
authors.lastname: McNabb
authors.forename: Jene A
authors.initials: JA
authors.lastname: Golovchenko
authors.forename: Jiali
authors.initials: J
authors.lastname: Li
mesh_terms: D004277:DNA, Single-Stranded / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation
chemical_list: D004277:DNA, Single-Stranded