Effects of salinity on biomarker responses in Crassostrea rhizophorae (Mollusca, Bivalvia) exposed to diesel oil.
Crassostrea rhizophorae is a euryhaline oyster that inhabits mangrove areas, which are widely distributed along the Brazilian coast. The aim of this study was to investigate the effects of salinity (9, 15, 25, and 35ppt) on the activities of glutathione S-transferase (GST), glucose 6-phosphate dehydrogenase (G6PDH), catalase (CAT), and acetylcholinesterase (AChE) in the digestive gland of this species after exposure to diesel oil for 7 days at nominal concentrations of 0.01, 0.1, and 1mlL(-1) and after depuration for 24h and 7 days. GST activity increased in a diesel oil concentration-dependent manner at salinities 25 and 15ppt and remained slightly elevated even after depuration periods of 24h and 7 days. No changes were observed in the activities of G6PDH, CAT, and AChE in the oysters exposed to diesel and depurated. Based on these results, GST activity in the digestive gland of C. rhizophorae might be used as a biomarker of exposure to diesel oil in sites where the salinity is between 15 and 25ppt, values usually observed in mangrove ecosystems.
da Silva
Angela Zaccaron
AZ
Laboratório de Biomarcadores de Contaminação Aquática e Imunoquímica, Departamento de Bioquímica, CCB, Universidade Federal de Santa Catarina, 88040-900, Florianópolis, SC, Brazil.
Zanette
Juliano
J
Fernando Ferreira
Jaime
J
Guzenski
João
J
Marques
Maria Risoleta Freire
MR
Bainy
Afonso Celso Dias
AC
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001938:Brazil; D002374:Catalase / Q000378:metabolism; D004064:Digestive System / Q000187:drug effects* / Q000201:enzymology; D005742:Gasoline / Q000633:toxicity*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005982:Glutathione Transferase / Q000032:analysis / Q000378:metabolism; D010125:Ostreidae / Q000187:drug effects* / Q000201:enzymology; D012492:Salts / Q000737:chemistry; D012623:Seawater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D005742:Gasoline; D012492:Salts; D014874:Water Pollutants, Chemical; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase