Clinical applications of microarray-based diagnostic tests.
Nearly 15 years have passed since the possibility of analyzing nucleic acid analytes in a massively parallel fashion was proposed using the then new concept of microarrays. A decade ago, proof of principle demonstration projects established the use of high density microarrays to genotype multiple polymorphisms within a large gene [cystic fibrosis transmembrance regulator (CFTR)], to rapidly analyze DNA sequences by hybridization and to ascertain differential gene expression of the entire genome of an organism. The use of microarrays has had an explosive influence on the rate at which new biological information can be learned, including in a nonhypothesis driven manner. The past decade has also seen these research tools applied increasingly to questions of clinical and medical relevance. Genotyping drug metabolizing enzyme genes, resequencing important tumor suppressor genes, and classifying neoplastic disease by differential gene expression profiles are but a few of the many possibilities to provide clinically useful information using microarray-based diagnostic tests.
Wu
Lin
L
Roche Molecular Diagnostics, Pleasanton, CA 94588, USA.
Williams
P Mickey
PM
Koch
Walter H
WH
D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D005786:Gene Expression Regulation; D016158:Genes, p53; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D010597:Pharmacogenetics / Q000295:instrumentation / Q000379:methods*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D003577:Cytochrome P-450 Enzyme System