title: Temporal cytokine gene expression patterns in subjects with trachoma identify distinct conjunctival responses associated with infection.
abstract: Ocular chlamydial disease is clinically diagnosed by the appearance of characteristic inflammatory changes and development of lymphoid follicles in the conjunctiva. Nucleic acid amplification tests and relatively non-invasive methods of sampling the conjunctival surface can be used to quantify the expression of chlamydial and host genes. Using quantitative real-time polymerase chain reaction to detect the presence of Chlamydia trachomatis (CT) 16S rRNA and human interleukin (IL)-1beta, IL-10, IL-12p40, interferon (IFN)-gamma and tumour necrosis factor (TNF)-alpha transcripts we examined the immune response at the conjunctival surface in a cohort of children living in a trachoma-endemic village in The Gambia. Elevated cytokine transcript levels were associated with the presence of CT 16S rRNA. Subclinical infection (CT infection without clinical signs of disease) elicited an immune response that is proinflammatory in nature, with elevations in the transcription of IL-1beta, IFN-gamma and IL-12p40. Clinically apparent infections were associated with the elevation of mRNA for the multi-functional cytokine TNF-alpha (fibrotic, type 1 inflammatory and regulatory) and the counter regulatory cytokine, IL-10, in addition to the other proinflammatory cytokines. A positive correlation between IFN-gamma transcript levels and the amount of CT 16S rRNA expressed in conjunctiva was found.
authors.affiliation: Medical Research Council Laboratories, Banjul, The Gambia, West Africa. nfaal@mrc.gm
authors.forename: N
authors.initials: N
authors.lastname: Faal
authors.forename: R L
authors.initials: RL
authors.lastname: Bailey
authors.forename: I
authors.initials: I
authors.lastname: Sarr
authors.forename: H
authors.initials: H
authors.lastname: Joof
authors.forename: D C W
authors.initials: DC
authors.lastname: Mabey
authors.forename: M J
authors.initials: MJ
authors.lastname: Holland
mesh_terms: D000293:Adolescent; D002648:Child; D002692:Chlamydia trachomatis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D003228:Conjunctiva / Q000276:immunology* / Q000382:microbiology; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D015870:Gene Expression; D006801:Humans; D008297:Male; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014141:Trachoma / Q000276:immunology*
chemical_list: D016207:Cytokines; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S