Peptide ligation by a reversible and reusable C-terminal thiol handle.
[reaction: see text] Peptide ligation of noncysteinyl residues can be achieved conveniently by a reversible C-terminal thiol handle together with a Ag(+) ion-assisted S,N-acyl migration. The regenerated C-terminal handle permits tandem ligation of multiple segment.
Lu
Yi-An
YA
Department of Biomedical Research, Scripps Research Institute, Jupiter, Florida 33458, USA.
Tam
James P
JP
D000215:Acylation; D000595:Amino Acid Sequence; D004952:Esters / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D004952:Esters; D010455:Peptides; D013438:Sulfhydryl Compounds; D012834:Silver