title: Enhanced productivity of human lysozyme by pH-controlled batch fermentation of recombinant Saccharomyces cerevisiae.
abstract: Human lysozyme (HLY) was successfully produced by Saccharomyces cerevisiae with a plasmid (pHK501) containing a synthetic HLY gene connected with a native secretion-signal sequence under control of the promoter for the glyceraldehydes-3-phosphate dehydrogenase gene. For large-scale production, batch fermentation was adopted with 1 l SD (Leu(-)) minimal medium in a 2-l jar fermentor. While pH-uncontrolled fermentation gave 42.3 units/ml of HLY over a period of 72-h cultivation, HLY production increased to 74.5 units/ml by performing pH-controlled fermentation, although a longer fermentation time was needed for maximal HLY production. A pH-shift strategy was designed in this study, resulting in higher HLY production and a shorter fermentation time.
authors.affiliation: International Center for Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Sun-Uk
authors.initials: SU
authors.lastname: Choi
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Paik
authors.forename: Seung-Cheol
authors.initials: SC
authors.lastname: Lee
authors.forename: Takuya
authors.initials: T
authors.lastname: Nihira
authors.forename: Yong-Il
authors.initials: YI
authors.lastname: Hwang