title: Terpenoid indole alkaloid production by Catharanthus roseus hairy roots induced by Agrobacterium tumefaciens harboring rol ABC genes.
abstract: We have established Catharanthus roseus hairy root cultures transgenic for the rol ABC genes from T(L)-DNA of the agropine-type Agrobacterium rhizogenes strain A4. The rol ABC hairy root lines exhibit a wild-type hairy root syndrome in terms of growth and morphology on solid medium. However, they differ from wild-type hairy root lines in that they more frequently have excellent adaptability to liquid medium and do not appear to form calli during cultivation. Moreover, they do not produce detectable levels of mannopine and agropine which, in contrast, are often synthesized abundantly in wild-type hairy root lines. The absence of these opines does not appear to cause the rol ABC lines to have higher levels of terpenoid indole alkaloids than wild-type hairy root lines. Unlike wild-type lines, rol ABC lines produce very similar levels of total alkaloids despite wide variations in individual alkaloid contents. This work demonstrates that the three genes rol ABC are sufficient to induce high-quality hairy roots in Catharanthus roseus.
authors.affiliation: Department of Biochemistry and Cell Biology, MS-140, 6100 Main St., Rice University, Houston, Texas 77005, USA.
authors.forename: Seung-Beom
authors.initials: SB
authors.lastname: Hong
authors.forename: Christie A M
authors.initials: CA
authors.lastname: Peebles
authors.forename: Jacqueline V
authors.initials: JV
authors.lastname: Shanks
authors.forename: Ka-Yiu
authors.initials: KY
authors.lastname: San
authors.forename: Susan I
authors.initials: SI
authors.lastname: Gibson
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000470:Alkaloids / Q000096:biosynthesis*; D030008:Catharanthus / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology; D002478:Cells, Cultured; D005798:Genes, Bacterial / Q000235:genetics; D007211:Indoles / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology; D013729:Terpenes / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D007211:Indoles; D013729:Terpenes