Effects of abiotic stresses on cell cycle progression in tobacco BY-2 cells.
Mild stresses such as high temperature (30 degrees C) or a low H2O2 concentration induced transient cell cycle arrest at G1/S or G2/M depending on the cell cycle stage at which the stress was applied. When stresses were introduced during G0 or G1, the G1/S checkpoint was mainly used; when stresses were introduced after S phase, G2/M was the primary checkpoint. The slowing of cell cycle progression was associated with transient delays in expression of A-, B-, and D-type cyclins. The delay in expression of NtcycA13, one of the A-type cyclins, was most pronounced. The levels of expression of Ntcyc29 (a cyclin B gene) and of CycD3-1 differed most depending on the applied stress, suggesting that different cellular adjustments to mild heat and a low concentration of H2O2 are reflected in the expression of these two cyclins.
Jang
Su Jin
SJ
Department of Biology, Sunchon National University, Sunchon 540-742, Korea.
Shin
Sung Hae
SH
Yee
Sung Tae
ST
Hwang
Baek
B
Im
Kyung Hoan
KH
Park
Ky Young
KY
D016590:Aphidicolin / Q000378:metabolism; D015152:Blotting, Northern; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics / Q000502:physiology*
D016213:Cyclins; D016590:Aphidicolin; D006861:Hydrogen Peroxide