Labeled Schwann cell transplantation: cell loss, host Schwann cell replacement, and strategies to enhance survival.
Although transplanted Schwann cells (SCs) can promote axon regeneration and remyelination and improve recovery in models of spinal cord injury, little is known about their survival and how they interact with host tissue. Using labeled SCs from transgenic rats expressing human placental alkaline phosphatase (PLAP), SC survival in a spinal cord contusion lesion was assessed. Few PLAP SCs survived at 2 weeks after acute transplantation. They died early due to necrosis and apoptosis. Delaying transplantation until 7 days after injury improved survival. A second wave of cell death occurred after surviving cells had integrated into the spinal cord. Survival of PLAP SCs was enhanced by immunosuppression with cyclosporin; delayed transplantation in conjunction with immunosuppression resulted in the best survival. In all cases, transplantation of SCs resulted in extensive infiltration of endogenous p75+ cells into the injury site, suggesting that endogenous SCs may play an important role in the repair observed after SC transplantation.
Hill
Caitlin E
CE
Miami Project to Cure Paralysis, Department of Neurological Surgery, University of Miami Miller School of Medicine, Miami, Florida 33136, USA.
Moon
Lawrence D F
LD
Wood
Patrick M
PM
Bunge
Mary Bartlett
MB
D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D002470:Cell Survival; D016572:Cyclosporine / Q000494:pharmacology; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D009336:Necrosis; D009580:Nitroblue Tetrazolium; D010920:Placenta / Q000201:enzymology; D051381:Rats; D011916:Rats, Inbred F344; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism; D012583:Schwann Cells / Q000502:physiology / Q000637:transplantation*; D013119:Spinal Cord Injuries / Q000473:pathology / Q000628:therapy
D007166:Immunosuppressive Agents; D020800:Receptor, Nerve Growth Factor; D009580:Nitroblue Tetrazolium; D016572:Cyclosporine; D000469:Alkaline Phosphatase