Widespread expression of liver receptor homolog 1 in mouse brain.
OBJECTIVE
The distribution of Liver receptor homolog 1 (LRH-1) mRNA was studied in mice brain with the aim to establish whether this nuclear hormone receptor is expressed also in the brain in addition to liver and classical steroidogenic tissues.


METHODS
Expression of LRH-1 mRNA in juvenile (30 days old) and adult (60 days old) mouse brain was examined using non-radioactive in situ hybridization with digoxigenin labeled cRNA probes and with RT PCR using specific primers.


RESULTS
LRH-1 was strongly expressed throughout the brain. Semiquantitative RT PCR revealed very strong expression of LRH-1 mRNA in cerebrum in comparison to liver and testis, and in situ hybridization revealed that LRH-1 mRNA is uniformly expressed in most brain areas.


CONCLUSIONS
LRH-1 is strongly expressed throughout the mouse brain suggesting important roles for this transcription factor, although its precise roles in the CNS remain to be elucidated.
Grgurevic
Neza
N
Center for animal genomics, Veterinary faculty, University of Ljubljana, Gerbiceva 60, SI-1000 Ljubljana, Slovenia.
Tobet
Stuart
S
Majdic
Gregor
G
D000375:Aging / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D004076:Digoxigenin; D017403:In Situ Hybridization; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015347:RNA Probes; D018075:RNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism
C431936:Nr5a2 protein, mouse; D015347:RNA Probes; D018075:RNA, Complementary; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D004076:Digoxigenin