title: Cardiac sarcolemma of the hamster. Enrichment of the (Na+ + K+)-ATPase.
abstract: Cardiac sarcolemma was prepared from normal hamsters using gentle homogenization, extraction with 0.6 M KCl and continuous density gradient centrifugation. The final fraction exhibited high (Na+ + K+)-ATPase activity (24 micromol/mg per h) and contained minimal contamination from mitochondria, myofibrils and lysosomes.
authors.affiliation: Department of Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond, Va 23298, USA.
authors.forename: D C
authors.initials: DC
authors.lastname: Pang
authors.forename: W B
authors.initials: WB
authors.lastname: Weglicki
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000379:methods; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D008247:Lysosomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism; D011189:Potassium Chloride / Q000737:chemistry; D012508:Sarcolemma / Q000737:chemistry*; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry*; D013395:Sucrose / Q000494:pharmacology
chemical_list: D013395:Sucrose; D011189:Potassium Chloride; D000254:Sodium-Potassium-Exchanging ATPase