Protection of mitochondrial integrity from oxidative stress by selenium-containing glutathione transferase.
The antioxidant activity of a novel artificial glutathione peroxidase-like enzyme, selenium-containing glutathione 5-transferase from Lucilia cuprina (seleno-LuGST1-1), was studied by using a ferrous sulfate/ascorbate-induced mitochondrial damage model system. Swelling of mitochondria, lipid peroxidation, and cytochrome-c oxidase activity were selected to evaluate the preservation of mitochondrial integrity in this system. Seleno-LuGST1-1 could effectively protect the mitochondria against oxidative damage in a dose-dependent manner and exhibited both higher catalytic activity and greater antioxidant ability than the classic mimic, 2-phenyl-1,2-benziososelenazol-3(2H)-one (Ebselen). This novel artificial biocatalyst therefore may have great potential for pharmacologic application in the treatment of reactive oxygen species-related diseases.
Yu
Huijun
H
Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun, People's Republic of China.
Liu
Junqiu
J
Li
Jing
J
Zang
Tianzhu
T
Luo
Guimin
G
Shen
Jiacong
J
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D003576:Electron Transport Complex IV / Q000187:drug effects / Q000378:metabolism; D005982:Glutathione Transferase / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008929:Mitochondria, Heart / Q000187:drug effects*; D008933:Mitochondrial Swelling / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D051140:Selenoproteins / Q000494:pharmacology*
D000975:Antioxidants; D051140:Selenoproteins; D003576:Electron Transport Complex IV; D005982:Glutathione Transferase