title: [Construction and expression of anti-keratin single-chain Fv antibody].
abstract: OBJECTIVE
  To construct an expression vector of human anti-keratin scFv and express functional scFv in E. coli.
  
  
  METHODS
  The V(H) and V(L) genes were amplified by PCR and cloned into a scFv expression vector, which was expressed in E. coli. The expression of scFv was tested by SDS-PAGE; The antigen-binding characteristics of scFv were tested by ELISA.
  
  
  RESULTS
  The V(H) and V(L) genes were cloned and expressed in E. coli successfully. The expressed scFv retained the antigen-binding characteristics of the parental antibody.
  
  
  CONCLUSIONS
  A functional anti-keratin scFv was obtained.
authors.affiliation: Department of Microbiology and Immunology, Capital University of Medical Science, Beijing 100054, China. erminzhang@sohu.com
authors.forename: Guo-min
authors.initials: GM
authors.lastname: Zhang
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Liu
mesh_terms: D000906:Antibodies / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D000918:Antibody Specificity / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D007633:Keratins / Q000276:immunology*; D019151:Peptide Library; D011485:Protein Binding
chemical_list: D000906:Antibodies; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D007633:Keratins