title: Inhibition of prohormone convertase 1 (PC1) expression in cholecystokinin (CCK) expressing At-T20 cells decreased cellular content and secretion of CCK and caused a shift in molecular forms of CCK secreted.
abstract: Two different RNAi methods were used to inhibit the expression of prohormone convertase 1 (PC1) in At-T20 cells. Transient transfection of double stranded RNA and stable expression of a vector expressing hairpin-loop RNA targeting PC1 reduced cholecystokinin (CCK) secretion from At-T20 cells. PC1 mRNA and protein were also decreased in the vector transfected cells. This treatment caused a shift in the forms of cholecystokinin (CCK) secreted, decreasing CCK 22 and increasing CCK 8. Stable expression of RNAi effectively decreased PC1 expression. The observed decrease in CCK seen with these RNAi treatments further supports a role for PC1 in CCK processing in these cells.
authors.affiliation: Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.
authors.forename: Margery C
authors.initials: MC
authors.lastname: Beinfeld
authors.forename: Daesety
authors.initials: D
authors.lastname: Vishnuvardhan
authors.forename: Alissa
authors.initials: A
authors.lastname: Blum
authors.forename: Nicole
authors.initials: N
authors.lastname: Reynolds
authors.forename: Sanya
authors.initials: S
authors.lastname: Fannous
authors.forename: Kouki
authors.initials: K
authors.lastname: Kitagawa
authors.forename: James E
authors.initials: JE
authors.lastname: Marchand
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002766:Cholecystokinin / Q000737:chemistry* / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D045664:Proprotein Convertase 1 / Q000096:biosynthesis / Q000172:deficiency* / Q000235:genetics; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D002766:Cholecystokinin; D045664:Proprotein Convertase 1