[Identification and characterization of synovial mesenchymal stem cells in temporomandibular joint].
OBJECTIVE
To identify and characterize mesenchymal stem cells from synovial membrane of temporomandibular joint in vitro.


METHODS
Synovial mesenchymal stem cells (SMSCs) were obtained by limited dilution method and expanded in 25 ml flasks. Methyl thiazolyl tetrazolium (MTT) method was used to determine the cell growth cycles. The expressions of vimentin and keratin were respectively detected with immunocytochemistry, while the expressions of CD8, CD34, CD44, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were determined by flow cytometry.


RESULTS
Pure mesenchymal stem cells were of spindle shape and uniform in size, which were intensively positive in vimentin, but negative in keratin. The expression of CD44, VCAM-1 and ICAM-1 were also verified by flow cytometry.


CONCLUSIONS
Mesenchymal stem cells could be purified from adult synovial membrane of temporomandibular joint.
Li
Jian
J
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
Long
Xing
X
Ke
Jin
J
Meng
Qing-gong
QG
Fang
Wei
W
D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D013583:Synovial Membrane / Q000166:cytology*; D013704:Temporomandibular Joint