Total antioxidant status in the blood serum of rats exposed to N-nitroso compounds and nitric oxide synthase inhibitors.
In this study, the total antioxidant status (TAS) was assayed in the blood serum of rats pretreated per os with either N-nitrosodiethylamine (NDEA) (0.1 mg/kg b.w./day) or N-methyl-N-nitrosourea (NMU) (0.1 mg/kg b.w./day) for 30 days. The animals were also dosed per os with spermidine (SPR) (10 mg/kg b.w./day) for a first 21 day period, and Nomega)-nitro-L-arginine methyl ester (L-NAME) (10 mg/kg b.w./day) given to animals for 3 days (days 22-24), respectively. Nitric oxide synthase (NOS) inhibitor, L-NAME was found to mitigate TAS levels in the blood serum of rats pretreated with NDEA and NMU. No such changes were found in animals dosed with L-NAME only nor even with L-NAME and spermidine, respectively. Since spermidine, also known as an inhibitor of iNOS synthesis, elevated TAS levels in rats dosed with L-NAME and NDEA/NMU, the polyamine was suggested to modify the NOS/NO origin to serve the physiological level of the total anti-oxidant status in rat blood serum.
Grudziński
Ireneusz P
IP
Department of Applied Physiology, Military Institute of Hygiene and Epidemiology, Kozielska 4, 01-163 Warsaw, Poland. I.Grudzinski@wihe.waw.pl
Frankiewicz-Jóźko
Anna
A
Szarska
Ewa
E
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004052:Diethylnitrosamine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008770:Methylnitrosourea / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D009603:Nitroso Compounds / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013095:Spermidine / Q000494:pharmacology; D013997:Time Factors
D000975:Antioxidants; D004791:Enzyme Inhibitors; D009603:Nitroso Compounds; D004052:Diethylnitrosamine; D008770:Methylnitrosourea; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D013095:Spermidine; D019331:NG-Nitroarginine Methyl Ester