title: Creating new genes by plasmid recombination in Escherichia coli and Bacillus subtilis.
abstract: Gene shuffling is a way of creating proteins with interesting new characteristics, starting from diverged sequences. We tested an alternative to gene shuffling based on plasmid recombination and found that Bacillus subtilis efficiently recombines sequences with 4% divergence, and Escherichia coli mutS is more appropriate for sequences with 22% divergence.
authors.affiliation: Eucodis GmbH, Wien, Austria.
authors.forename: Ana
authors.initials: A
authors.lastname: Gomez
authors.forename: Tatjana
authors.initials: T
authors.lastname: Galic
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Mariet
authors.forename: Ivan
authors.initials: I
authors.lastname: Matic
authors.forename: Miroslav
authors.initials: M
authors.lastname: Radman
authors.forename: Marie-Agnès
authors.initials: MA
authors.lastname: Petit
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005821:Genetic Techniques; D051719:MutS DNA Mismatch-Binding Protein / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D029968:Escherichia coli Proteins; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli