Fabrication of high quality microarrays.
Fabrication of DNA microarray demands that between ten (diagnostic microarrays) and many hundred thousands of probes (research or screening microarrays) are efficiently immobilised to a glass or plastic surface using a suitable chemistry. DNA microarray performance is measured by parameters like array geometry, spot density, spot characteristics (morphology, probe density and hybridised density), background, specificity and sensitivity. At least 13 factors affect these parameters and factors affecting fabrication of microarrays are used in this review to compare different fabrication methods (spotted microarrays and in situ synthesis of microarrays) and immobilisation chemistries.
Dufva
Martin
M
Microarray Technology Group, Department of Micro and Nanotechnology, Technical University of Denmark, Oersteds Plads, Bld. 345 east, DK-2800 Kongens Lyngby, Denmark. mdu@mic.dtu.dk
D003432:Cross-Linking Reagents / Q000737:chemistry*; D046228:Microarray Analysis; D046208:Microchip Analytical Procedures; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000737:chemistry*; D012680:Sensitivity and Specificity
D003432:Cross-Linking Reagents; D015345:Oligonucleotide Probes