A convenient method for the synthesis of oligonucleotide-cationic peptide conjugates.
A method was developed for the synthesis of oligonucleotide-cationic peptide conjugates in solution phase by disulfide bond formation. Precipitation was avoided by the easily removable triethylammonium trifluoroacetate (TEATFAc) salt which served at the same time as a buffer of the reaction mixture. The fast and high yielding disulfide bond formation was due to the Npys thio protecting and activating group of Cys. A solution of the free 5-thiol modified oligonucleotide obtained from Poly-Pak purification was used for conjugation.
Ferenc
G
G
Department of Medicinal Chemistry, University of Szeged, Szeged, Dóm tir 8, Hungary.
Kupihár
Z
Z
Kele
Z
Z
Kovács
L
L
D001483:Base Sequence; D002021:Buffers; D002412:Cations / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000737:chemistry; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012492:Salts / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D002021:Buffers; D002412:Cations; D004220:Disulfides; D009841:Oligonucleotides; D010455:Peptides; D012492:Salts