Ectomycorrhizal symbiosis in vitro between Tricholoma matsutake and Pinus densiflora seedlings that resembles naturally occurring 'shiro'.
We established an in vitro ectomycorrhizal symbiosis between Tricholoma matsutake and Pinus densiflora. Mycorrhiza formed in a substrate of Modified Norkrans' C medium and granite-based soil had features similar to those observed previously only in naturally occurring mycorrhizal system called 'shiro,' and promoted the growth of plants with smaller root/shoot ratios. The in vitro formation of 'shiro' is essential for the development of T. matsutake system to produce mushrooms and is useful for the propagation and plantation of the mycorrhizal seedlings.
Yamada
Akiyoshi
A
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Minami-minowa, Nagano, 399-4598, Japan. akiyosh@gipmc.shinshu-u.ac.jp.
Maeda
Ken
K
Ibaraki Prefectural Forestry Institute, Naka, Ibaraki, 311-0122, Japan.
Kobayashi
Hisayasu
H
Ibaraki Prefectural Forestry Institute, Naka, Ibaraki, 311-0122, Japan.
Murata
Hitoshi
H
Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, 305-8687, Japan.
D003470:Culture Media; D038821:Mycorrhizae / Q000502:physiology*; D028223:Pinus / Q000254:growth & development / Q000382:microbiology*; D012822:Silicon Dioxide; D013559:Symbiosis
D003470:Culture Media; C007886:granite; D012822:Silicon Dioxide