[Dynamic observation of lung metastasis model of adenoid cystic carcinoma].
OBJECTIVE
To form ACC-M-GFP cells by transfecting pEGFP-1 into ACC-M cells, and to build up lung metastasis model of adenoid cystic carcinoma for dynamic observation in simulated lung environment.


METHODS
pEGFP-1 was prepared and then transfected into ACC-M cell lines by using cationic lipid-based gene transfer technique. After successive selection, the ACC-M-GFP cells were collected and inoculated in BALB/C mice. The simulated lung environment was built up, and dynamic observation was performed by laser confocal microscopy within four weeks after transfection.


RESULTS
The amount of fluorescent tumor cell colonies and the intensity of fluorescence gradually increased from the second week to the forth week after transfection. In the meantime, only a very small number of tumor cells had the ability to form clones.


CONCLUSIONS
We successfully built up a lung metastasis model of adenoid cystic carcinoma for dynamic observation. The model is suitable for capturing and analyzing metastatic tumor cells in early stage.
Chen
Shao-wei
SW
Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Sun Yet-sen University, Guangzhou 510055, China.
Huang
Hong-zhang
HZ
Liu
Xi-qiang
XQ
D000818:Animals; D003528:Carcinoma, Adenoid Cystic / Q000556:secondary*; D045744:Cell Line, Tumor; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000473:pathology*; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental; D014162:Transfection
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins