title: Renal ischemia/reperfusion injury inhibits differentiation of dendritic cells derived from bone marrow monocytes in rats.
abstract: Dendritic cells (DCs) are impacted by surgical injury, exercise, and other physiological stressors. This study aims to determine whether renal I/R injury affects 1) the differentiation of myeloid DCs from bone marrow monocytes (BMMos) and the maturation and activation state of these DCs and 2) DC infiltration of kidney. Sprague-Dawley rats were subjected to I/R injury or sham-operated. Creatinine clearance was monitored daily during the 14 d of reperfusion that followed the ischemic insult. At 2 and 14 d of reperfusion, the following were assessed 1) properties of BMMo-derived DCs (i.e., the amount of generated DCs, differentiation state markers [CD11c, CD80, CD86, and Ia], and functional state [MLR and amount of IL-12 produced]), and 2) the presence of DCs in the kidney. Numbers of BMMo-derived DCs were significantly decreased in the I/R injured group (compared with the sham-operated group) at 2 d but not 14 d. A comparison of the their functionality found mixed lymphocyte response [MLR] and IL-12 production were similar in the two groups at both time points. Also, immunohistochemistry showed infiltrating DCs in the outer medulla of the I/R injured kidney at 2 d but not 14 d of reperfusion. Thus, I/R stress reduces the number of DCs differentiated from BMMos but not the functional activity of these DCs. This decrease may reflect a stress-induced downshift in the capacity of BMMos to differentiate into DCs and a parallel upshift in the capacity of DCs to infiltrate the kidney.
authors.affiliation: Division of Nephrology, Mackay Memorial Hospital, Taipei, Taiwan.
authors.forename: Chih-Jen
authors.initials: CJ
authors.lastname: Wu
authors.forename: Joen-Rong
authors.initials: JR
authors.lastname: Sheu
authors.forename: Han-Hsiang
authors.initials: HH
authors.lastname: Chen
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Liao
authors.forename: Yuh-Cheng
authors.initials: YC
authors.lastname: Yang
authors.forename: Stone
authors.initials: S
authors.lastname: Yang
authors.forename: Yu-Jen
authors.initials: YJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology* / Q000648:ultrastructure; D002454:Cell Differentiation / Q000502:physiology; D003713:Dendritic Cells / Q000502:physiology* / Q000648:ultrastructure; D005434:Flow Cytometry; D007150:Immunohistochemistry; D018664:Interleukin-12 / Q000378:metabolism; D007668:Kidney / Q000473:pathology*; D007677:Kidney Function Tests; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology*
chemical_list: D018664:Interleukin-12