Biophotonic hydrogen peroxide production by antibodies, T cells, and T-cell membranes.
Rapidly accumulating evidence indicates that inflammatory T cells sensitively respond to their redox environment by activating signal transduction pathways. The hypothesis that T-cell receptors have the potential to catalytically transform singlet oxygen into H(2)O(2) attracted our attention since the biophysical regulation of this process would provide a new tool for therapeutically directing T cells down a preferred signaling pathway. Light-dependent production of H(2)O(2) was first described in antibodies, and we reproduced these findings. Using a real-time H(2)O(2) sensor we extended them by showing that the reaction proceeds in a biphasic way with a short-lived phase that is fast compared to the slow second phase of the reaction. We then showed that Jurkat T cells biophotonically produce about 30nM H(2)O(2)/min/mg protein when pretreated with NaN(3). This activity was concentrated 4 to 5 times in T-cell membrane preparations. The implications of these observations for the development of new therapeutic tools for inflammatory diseases are discussed.
Waite
Gabi Nindl
GN
Cellular and Integrative Physiology, Indiana University School of Medicine, Terre Haute, IN 47809, USA. gnindl@iupui.edu
Waite
Lee R
LR
Hughes
Ellen F
EF
Balcavage
Walter X
WX
D000906:Antibodies / Q000737:chemistry* / Q000528:radiation effects; D002462:Cell Membrane / Q000378:metabolism* / Q000528:radiation effects*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000378:metabolism* / Q000528:radiation effects; D019169:Jurkat Cells; D008027:Light; D011829:Radiation Dosage; D013601:T-Lymphocytes / Q000378:metabolism* / Q000528:radiation effects*
D000906:Antibodies; D006861:Hydrogen Peroxide