title: Design of Polyphosphoester-DNA Nanoparticles for Non-Viral Gene Delivery.
abstract: Development of safe and effective non-viral gene carriers is still critical to the ultimate success of gene therapy. This review highlights our attempt to design the gene carriers in a systematic manner. We have synthesized a series of polymers with a phosphoester backbone containing different charge groups in the sidechain connected to the backbone through a phosphate (PO) or a phosphoramide (PN) bond. These gene carriers have different charge groups, sidechain lengths, and branching structures, but they are structurally related to allow a systematic investigation of the structure-property relationship, including DNA binding capacity, cytotoxicity, DNA protection, biodegradability, DNA release kinetics, and transfection efficiency.
authors.affiliation: Department of Materials Science and Engineering The Johns Hopkins University, Baltimore, Maryland 21205.
authors.forename: Hai-Quan
authors.initials: HQ
authors.lastname: Mao
authors.forename: Kam W
authors.initials: KW
authors.lastname: Leong