Determination of trace aluminum by fluorescence quenching method based on catalysis of potassium chlorate oxidizing alizarin red.
A new method for the determination of trace aluminum has been proposed. It is based on the fact that alizarin red can emit strong and stable fluorescence at 80 degrees C for 30 min and Al(3+) can effectively catalyze potassium chlorate oxidizing alizarin red to form non-fluorescence complex which cause the fluorescence quenching. The linear dynamic range of this method is 0.040-4.00 ngl(-1) with a detection limit of 5.3 pgl(-1). The regression equation can be expressed as DeltaI(f)=8.731+21.73c(Al(3+)) (ngl(-1)), with the correlation coefficient r=0.9992 (n=6). This sensitive, rapid and accurate method has been applied to the determination of trace aluminum(III) in human hair and tea samples successfully. What is more, the mechanism of catalyzing potassium chlorate oxidizing alizarin red by the fluorescence quenching method is also discussed.
Shao-Qin
Lin
L
Department of Biochemistry, Fujian Education College, Fuzhou 350001, China.
Xuan
Lin
L
Shi-Rong
Hu
H
Li-Qing
Zeng
Z
Yan
Wang
W
Li
Chen
C
Jia-Ming
Liu
L
Long-Di
Li
L
D000535:Aluminum / Q000032:analysis*; D000880:Anthraquinones / Q000737:chemistry*; D002384:Catalysis; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D010472:Perchlorates; D017680:Potassium Compounds; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013816:Thermodynamics; D014131:Trace Elements / Q000032:analysis
D000880:Anthraquinones; D010472:Perchlorates; D017680:Potassium Compounds; D014131:Trace Elements; C009006:potassium perchlorate; C010078:alizarin; D000535:Aluminum