2'-bis-pyrene modified oligonucleotides: sensitive fluorescent probes of nucleic acids structure.
A new type of fluorescent nucleic acid probes, 2-bis-pyrene-modified oligonucleotides, is described. Preparation of these conjugates involves attachment of two pyrene moieties to the 2'-phosphate group introduced into any position within a sequence by solid-phase phosphoramidite synthesis. Good hybridization properties of the 2'-bis-pyrene probes, their nuclease resistance and sensitivity of fluorescence to the type of complementary nucleic acid have been demonstrated.
Novopashina
D S
DS
Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia. danov@niboch.nsc.ru
Stetsenko
D A
DA
Totskaya
O S
OS
Repkova
M N
MN
Venyaminova
A G
AG
D004247:DNA / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D010710:Phosphates; D011721:Pyrenes; D012313:RNA; D004247:DNA