Identification of a positive Cis-element upstream of human NKX3.1 gene.
NKX3.1 is a prostate-specific homeobox gene related to prostate development and prostate cancer. In this work, we aimed to identify precisely the functional cis-element in the 197 bp region (from -1032 to -836 bp) of the NKX3.1 promoter (from -1032 to +8 bp), which was previously identified to present positive regulatory activity on NKX3.1 expression, by deletion mutagenesis analysis and electrophoretic mobility shift assay (EMSA). A 16 bp positive cis-element located between -920 and -905 bp upstream of the NKX3.1 gene was identified by deletion mutation analysis and proved to be a functional positive cis-element by EMSA. It will be important to further study the functions and regulatory mechanisms of this positive cis-element in NKX3.1 gene expression.
Jiang
An-Li
AL
Department of Biochemistry, Medical School of Shandong University, Jinan 250012, China.
Zhang
Peng-Ju
PJ
Hu
Xiao-Yan
XY
Chen
Wei-Wen
WW
Kong
Feng
F
Liu
Zhi-Fang
ZF
Yuan
Hui-Qing
HQ
Zhang
Jian-Ye
JY
D001483:Base Sequence; D045744:Cell Line, Tumor; D002874:Chromosome Mapping / Q000379:methods*; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011471:Prostatic Neoplasms / Q000235:genetics*; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D014157:Transcription Factors / Q000235:genetics*
D018398:Homeodomain Proteins; C491178:NKX3-1 protein, human; D014157:Transcription Factors