Resolution of the vertical and horizontal heterogeneity of adsorbed collagen layers by combination of QCM-D and AFM.
Collagen (type I from calf skin) adsorption on polystyrene (PS) and plasma-oxidized polystyrene (PSox) was studied, using a quartz crystal microbalance with energy dissipation measurements (QCM-D) and atomic force microscopy (AFM) in tapping mode. Radio-labeled collagen was used to measure the adsorbed amount and the ability of adsorbed collagen to exchange with molecules in the solution. The results show that the collagen adlayer consists of two parts: a dense and thin sheet in which fibrils are formed (directly observed by AFM) and an overlying thick layer (up to 200 nm) containing protruding molecules or bundles which are in very low concentration but modify noticeably the local viscosity. The thickness and viscosity of the semi-liquid adlayer both increase with adsorption time and collagen concentration. Fibril formation near the surface also increases with time and collagen concentration and occurs more readily on PS compared to PSox. Radiochemical measurements show that this may be related to the larger mobility of molecules adsorbed on PS, presumably owing to a smaller number of binding points.
Gurdak
Elzbieta
E
Unité de Chimie des Interfaces, Université Catholique de Louvain, Croix du Sud 2/18, Belgium.
Dupont-Gillain
Christine C
CC
Booth
John
J
Roberts
Clive J
CJ
Rouxhet
Paul G
PG
D000818:Animals; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D011137:Polystyrenes / Q000737:chemistry; D011854:Radiochemistry
D011137:Polystyrenes; D003094:Collagen