title: [Rhinovirus and acute bronchiolitis in young infants].
abstract: OBJECTIVE
  To determine the prevalence of rhinovirus infection in hospitalized young infants with acute bronchiolitis.
  
  
  METHODS
  Hospitalized children with acute bronchiolitis admitted to the Hospital São Lucas/PUCRS between May and September 2002 were selected prospectively. Nasopharyngeal samples were assayed for respiratory syncytial virus, parainfluenza, influenza and adenovirus by immunofluorescence. For rhinovirus test a reverse transcription-polymerase chain reaction for picornavirus was used, followed by hybridization with rhinovirus specific probes.
  
  
  RESULTS
  Forty-five patients were selected for the study. The median age of the subjects studied was 2 months. Positive samples for respiratory viruses were found in 35/45 (77.8%) subjects and 7/35 (20%) patients had dual infection. Respiratory syncytial virus was detected in 33/35 (94%) cases. Rhinovirus was detected in 6/35 patients (17%).
  
  
  CONCLUSIONS
  Rhinovirus was the second most common agent detected in nasal secretions from young infants hospitalized with acute bronchiolitis.
authors.affiliation: Departamento de Pediatria, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil. pmpitrez@pucrs.br
authors.forename: Paulo M C
authors.initials: PM
authors.lastname: Pitrez
authors.forename: Renato T
authors.initials: RT
authors.lastname: Stein
authors.forename: Larissa
authors.initials: L
authors.lastname: Stuermer
authors.forename: Izolete S
authors.initials: IS
authors.lastname: Macedo
authors.forename: Virgínia M
authors.initials: VM
authors.lastname: Schmitt
authors.forename: Marcus H
authors.initials: MH
authors.lastname: Jones
authors.forename: Eurico
authors.initials: E
authors.lastname: Arruda
mesh_terms: D000208:Acute Disease; D001938:Brazil / Q000453:epidemiology; D001988:Bronchiolitis / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010361:Patients; D010850:Picornaviridae Infections / Q000453:epidemiology*; D015995:Prevalence; D011446:Prospective Studies; D012135:Respiratory Sounds; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology*; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012229:Rhinovirus / Q000302:isolation & purification; D018709:Statistics, Nonparametric