title: A BAC library of the East African haplochromine cichlid fish Astatotilapia burtoni.
abstract: A BAC library was constructed from Astatotilapia burtoni, a haplochromine cichlid that is found in Lake Tanganyika, East Africa, and its surrounding rivers. The library was generated from genomic DNA of blood cells and comprises 96,768 individual clones. Its median insert size is 150 kb and the coverage is expected to represent about 14 genome equivalents. The coverage evaluation was based on genome size estimates that were obtained by flow cytometry. In addition, hybridization screens with five probes largely corroborate the above coverage estimate, although the number of clones ranged from 5 to 22 authenticated clones per single copy probe. The BAC library described here is expected to be useful to the scientific community interested in cichlid genomics as an important resource to gain new insights into the rapid evolution of the great species diversity of haplochromine cichlid fishes.
authors.affiliation: Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, 78457 Konstanz, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Lang
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Miyake
authors.forename: Ingo
authors.initials: I
authors.lastname: Braasch
authors.forename: Deborah
authors.initials: D
authors.lastname: Tinnemore
authors.forename: Nicol
authors.initials: N
authors.lastname: Siegel
authors.forename: Walter
authors.initials: W
authors.lastname: Salzburger
authors.forename: Chris T
authors.initials: CT
authors.lastname: Amemiya
authors.forename: Axel
authors.initials: A
authors.lastname: Meyer
mesh_terms: D000351:Africa, Eastern; D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D023681:Cichlids / Q000235:genetics*; D016000:Cluster Analysis; D017931:DNA Primers; D005434:Flow Cytometry; D015723:Gene Library; D010802:Phylogeny; D013045:Species Specificity
chemical_list: D017931:DNA Primers