[Effect of salidroside on mitochondria injury induced by sodium azide].
OBJECTIVE
To study the protective effect of salidroside on mitochondria injury induced by sodium azide.


METHODS
Human neuroblastoma SH-SY5Y cells were exposed to sodium azide with different concentration of salidroside, then cell viability was measured by thiazolyl blue (MTT) method and mitochondrial membrane potential (MMP) was detected by JC-1 method. Protective effect of salidroside against disfunction of mitochondria induced by sodium azide was detected by resazurin method.


RESULTS
After exposing to 64 mmol x L(-1) sodium azide for 4 hours, cell viability and MMP of SH-SY5Y cells significantly decreased. When pretreated with salidroside, the cell damage was greatly reduced and the mitochondrial membrane potential was maintained. Furthermore, salidroside can protect function of rat brain mitochondria against damage induced by sodium azide.


CONCLUSIONS
Salidroside was demonstrated to play an important role in improving the function of mitochondria.
Cao
Li-li
LL
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Du
Guan-hua
GH
Wang
Min-wei
MW
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology* / Q000503:physiopathology; D045744:Cell Line, Tumor; D002470:Cell Survival; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009447:Neuroblastoma / Q000473:pathology* / Q000503:physiopathology; D010084:Oxidation-Reduction / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D029771:Rhodiola / Q000737:chemistry; D019810:Sodium Azide
D005960:Glucosides; D010636:Phenols; D019810:Sodium Azide; C009172:rhodioloside