title: Tissue microarrays from HOPE-fixed specimens allow for enhanced high throughput molecular analyses in paraffin-embedded material.
abstract: In this study, we describe a technique that allows for the production and the use of tissue microarrays (TMA) from HOPE-fixed, paraffin-embedded specimens. The combination of these two technologies unites the advantages of the high throughput aspects in TMA, with good preservation of nucleic acids, proteins, and morphology of HOPE-fixed tissues, thus substantially widening the capabilities presently available for molecular studies in paraffin-embedded tissues.
authors.affiliation: Research Center Borstel, Clinical and Experimental Pathology, Germany. tgoldmann@fz-borstel.de
authors.forename: Torsten
authors.initials: T
authors.lastname: Goldmann
authors.forename: Daniel
authors.initials: D
authors.lastname: Drömann
authors.forename: Marouane
authors.initials: M
authors.lastname: Marzouki
authors.forename: Udo
authors.initials: U
authors.lastname: Schimmel
authors.forename: Karsten
authors.initials: K
authors.lastname: Debel
authors.forename: Detlev
authors.initials: D
authors.lastname: Branscheid
authors.forename: Tobias
authors.initials: T
authors.lastname: Zeiser
authors.forename: Jan
authors.initials: J
authors.lastname: Rupp
authors.forename: Johannes
authors.initials: J
authors.lastname: Gerdes
authors.forename: Peter
authors.initials: P
authors.lastname: Zabel
authors.forename: Ekkehard
authors.initials: E
authors.lastname: Vollmer
mesh_terms: D015415:Biomarkers; D001706:Biopsy; D005260:Female; D005404:Fixatives; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization; D008967:Molecular Biology; D016612:Paraffin Embedding; D012333:RNA, Messenger / Q000378:metabolism; D046888:Tissue Array Analysis / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D015415:Biomarkers; D005404:Fixatives; D012333:RNA, Messenger