title: TLA-2, a novel Ambler class A expanded-spectrum beta-lactamase.
abstract: Beta-lactamase TLA-2 is encoded by a 47-kb plasmid isolated from an unidentified bacterial strain from a wastewater treatment plant. TLA-2 is an Ambler class A beta-lactamase that shares 52% amino acid identity with CGA-1 from Chryseobacterium gleum and 51% with TLA-1 from Escherichia coli. The enzyme hydrolyzes mostly cephalosporins.
authors.affiliation: Service de Bactériologie-Virologie, Université Paris XI, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Girlich
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Andreas
authors.initials: A
authors.lastname: Schlüter
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000187:drug effects; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012722:Sewage / Q000382:microbiology*; D001618:beta-Lactamases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D012722:Sewage; D001618:beta-Lactamases