DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii.
Infection by nosocomial pathogenic bacteria is increasingly becoming a major threat to the patients in the hospital. We have developed a diagnostic DNA microarray for the detection of two important nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii. The diagnostic DNA microarray contains the species-specific probes of 15mer oligonucleotides designed based on the sequences of 23S ribosomal DNA. The performance of DNA microarray in diagnosing P. aeruginosa and A. baumannii was evaluated using reference bacteria as well as clinical specimens such as blood, stool, pus, sputum, urine and cerebrospinal fluid. Using this DNA microarray, A. baumannii could be successfully detected in 11 out of 13 clinical specimens, thus giving the sensitivity of 84.6% with the specificity of 100% and the positive predictive value of 100%. P. aeruginosa could also be detected in 25 out of 26 clinical specimens, showing the sensitivity of 96.2%, the specificity of 100%, and the positive predictive value of 100%. These results suggest that two nosocomial pathogens, P. aeruginosa and A. baumannii, can be efficiently diagnosed by using the DNA microarray developed in this study.
Keum
Ki Chang
KC
Department of Radiation Oncology, Yonsei University College of Medicine, Seoul, South Korea.
Yoo
Seung Min
SM
Lee
Sang Yup
SY
Chang
Kyung Hee
KH
Yoo
Nae Choon
NC
Yoo
Won Min
WM
Kim
June Myung
JM
Choi
Jun Yong
JY
Kim
Jong Seok
JS
Lee
Gene
G
D040981:Acinetobacter baumannii / Q000235:genetics*; D003428:Cross Infection / Q000382:microbiology*; D006801:Humans; D025202:Molecular Diagnostic Techniques; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D015203:Reproducibility of Results