title: Increased rate of DNA recovery from United Kingdom epidemic Clostridium difficile PCR ribotype 1 strains stored cryogenically.
abstract: We noted that some Clostridium difficile isolates are nonrecoverable after frozen storage and so used molecular typing analysis to characterize DNA from these strains. The recovery rate of C. difficile PCR ribotype 1 was statistically significantly greater than that of other strains. This observation has implications for C. difficile epidemiological studies.
authors.affiliation: Department of Microbiology, Leeds General Infirmary, The Old Medical School, Thoresby Place, Leeds LS1 3EX, United Kingdom.
authors.forename: N J
authors.initials: NJ
authors.lastname: Asha
authors.forename: W N
authors.initials: WN
authors.lastname: Fawley
authors.forename: J
authors.initials: J
authors.lastname: Freeman
authors.forename: M H
authors.initials: MH
authors.lastname: Wilcox
mesh_terms: D016360:Clostridioides difficile / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D015925:Cryopreservation; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D006801:Humans; D012189:Retrospective Studies; D021521:Ribotyping; D006113:United Kingdom
chemical_list: D004269:DNA, Bacterial