title: [Inhibitory effects of chloride channel blockers NPPB on proliferation of human glomerular mesangial cells].
abstract: OBJECTIVE
  To investigate the effects of NPPB, a chloride channel blocker, on proliferation of mesangial cells.
  
  
  METHODS
  Cell proliferation was determined by measuring cell number and 3H-thymidine incorporation. The LDH activity released from these cells was measured as evaluation of cell viability. The phase of cell cycle was detected by flow cytometry.
  
  
  RESULTS
  Cell proliferation assays showed that treatment with both NPPB (50 and 25 micromol x L(-1)) and in hypertonic media (100% increased osmolarity with D-mannitol ) significantly reduced the number of human MC and 3H-thymidine incorporation in a dose-dependent manner. But the LDH activity was not significantly altered in the treatment with 50 micromol x L(-1) NPPB. Flow cytometry experiments showed that 50 and 25 micromol x L(-1) NPPB arrested (84.2 +/- 2.4) % and (80.8 +/- 2.9) % of cells at G0/G1 stage, versus (70.5 +/- 1.4) % of control cells. Conclusion NPPB suppresses cell proliferation and produces growth arrest at G0/G1 phase in human MC by a mechanism probably associated with changes in cell volume.
authors.affiliation: Department of Pharmacology, Harbin Medical University, Bio-pharmaceutical Key Laboratory of Heilongjiang Province, China.
authors.forename: Jun-dong
authors.initials: JD
authors.lastname: Jiao
authors.forename: Peng
authors.initials: P
authors.lastname: Yue
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Du
authors.forename: De-li
authors.initials: DL
authors.lastname: Dong
authors.forename: Jing
authors.initials: J
authors.lastname: Ai
authors.forename: Bao-feng
authors.initials: BF
authors.lastname: Yang
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D018118:Chloride Channels / Q000037:antagonists & inhibitors*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D050527:Mesangial Cells / Q000166:cytology* / Q000378:metabolism; D009579:Nitrobenzoates / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D018118:Chloride Channels; D009579:Nitrobenzoates; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D007770:L-Lactate Dehydrogenase