title: Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant.
abstract: Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defect of a B. pseudomallei bimA mutant. While the BimA homologs differ in their amino-terminal sequence, they interact directly with actin in vitro and vary in their ability to bind Arp3.
authors.affiliation: Division of Microbiology, Institute for Animal Health, Compton Laboratory, Berkshire, UK.
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Stevens
authors.forename: Ricky L
authors.initials: RL
authors.lastname: Ulrich
authors.forename: Lowrie A
authors.initials: LA
authors.lastname: Taylor
authors.forename: Michael W
authors.initials: MW
authors.lastname: Wood
authors.forename: David
authors.initials: D
authors.lastname: Deshazer
authors.forename: Mark P
authors.initials: MP
authors.lastname: Stevens
authors.forename: Edouard E
authors.initials: EE
authors.lastname: Galyov
mesh_terms: D051378:Actin-Related Protein 3 / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D019117:Burkholderia / Q000235:genetics* / Q000502:physiology*; D042726:Burkholderia mallei / Q000235:genetics / Q000502:physiology; D016957:Burkholderia pseudomallei / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D017353:Gene Deletion; D005816:Genetic Complementation Test; D015151:Immunoblotting; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics* / Q000502:physiology; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid
chemical_list: D051378:Actin-Related Protein 3; D001426:Bacterial Proteins; C503173:BimA protein, Burkholderia pseudomallei; D008840:Microfilament Proteins