Heterogeneity of putative surface layer proteins in Lactobacillus helveticus.
The S-layer-encoding genes of 21 Lactobacillus helveticus strains were characterized. Phylogenetic analysis based on the identified S-layer genes revealed two main clusters, one which includes a sequence similar to that of the slpH1 gene of L. helveticus CNRZ 892 and a second cluster which includes genes similar to that of prtY. These results were further confirmed by Southern blot hybridization. This study demonstrates S-layer gene variability in the species L. helveticus.
Gatti
Monica
M
Department of Genetic Anthropology Evolution, Parco Area delle Scienze 11A, University of Parma, 43100 Parma, Italy. monica.gatti@unipr.it
Rossetti
Lia
L
Fornasari
Maria Emanuela
ME
Lazzi
Camilla
C
Giraffa
Giorgio
G
Neviani
Erasmo
E
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D014644:Genetic Variation; D052198:Lactobacillus helveticus / Q000145:classification* / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D005810:Multigene Family; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; C055764:S-layer proteins