title: Growth characteristics of canine pathogenic viruses in MDCK cells cultured in RPMI 1640 medium without animal protein.
abstract: Madin Darby canine kidney (MDCK) cells were adapted to serum-free RPMI 1640 medium and used for cultivation of canine viruses. RPMI 1640 medium was supplemented with a soybean peptone, L-glutamine and antibiotics, so that the protein concentration was less than 5 microg/ml (RPMI/SP medium). The resulting adapted MDCK-SP cells showed steady growth after the twenty-eighth passage in RPMI/SP medium (MDCK-SP cell culture). Canine distemper virus, canine parvovirus, canine adenoviruses and canine parainfluenza virus, which are the principal components of canine combined virus vaccines, grew in the MDCK-SP cell culture as efficiently as the parental MDCK cells cultured in the conventional Eagle's MEM containing fetal bovine serum. Consequently, the use of MDCK-SP cell culture can make current canine vaccine products much safer, of higher quality and at lower cost.
authors.affiliation: Laboratory of Clinical Microbiology, Kyoritsu Seiyaku Corporation, Kudankita 1-12-4, Tokyo 102-0073, Japan. msmmchzk@mb.infoweb.ne.jp
authors.forename: Masami
authors.initials: M
authors.lastname: Mochizuki
mesh_terms: D006516:Adenoviruses, Canine / Q000254:growth & development*; D000818:Animals; D002460:Cell Line; D016895:Culture Media, Serum-Free; D004217:Distemper Virus, Canine / Q000254:growth & development*; D004285:Dogs; D018098:Paramyxovirinae / Q000254:growth & development*; D017993:Parvovirus, Canine / Q000254:growth & development*; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D016895:Culture Media, Serum-Free