Fibroblast growth factor-2 stimulates hyaluronan production by human dental pulp cells.
Hyaluronan (HA), is a high molecular mass extracellular matrix constituting connective tissue and plays a critical role in not only homeostasis but also inflammatory and wound-healing responses. In this study, we investigated the effect of fibroblast growth factor (FGF)-2 on the production of HA by human dental pulp cells (HDPC). An inhibition binding-protein assay showed that FGF-2 increased HA production by HDPC. In addition, expression of mRNA of hyaluronan synthase (HAS) 1 and HAS 2, both of which are related to the production of high molecular mass of HA, but not HAS 3, was enhanced in FGF-2-stimulated HDPC. These results provide new evidence for the involvement of FGF-2 in the regulation of HA production by HDPC possibly through HAS 1 and HAS 2.
Shimabukuro
Yoshio
Y
Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, Osaka, Japan. syoshio@dent.osaka-u.ac.jp
Ueda
Maki
M
Ichikawa
Tomoo
T
Terashi
Yoshimitsu
Y
Yamada
Satoru
S
Kusumoto
Yutaka
Y
Takedachi
Masahide
M
Terakura
Mami
M
Kohya
Akira
A
Hashikawa
Tomoko
T
Murakami
Shinya
S
D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D014453:Glucuronosyltransferase / Q000032:analysis / Q000187:drug effects; D006801:Humans; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis
D007527:Isoenzymes; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D006820:Hyaluronic Acid; D014453:Glucuronosyltransferase; C101754:HAS2 protein, human; C105458:HAS3 protein, human; D000076002:Hyaluronan Synthases