title: [Influence of IL-2 on proliferation of cultured human pituitary adenoma cells and its mechanism].
abstract: OBJECTIVE
  To investigate the effects of IL-2 on the proliferation, cell cycle, protein kinase C(PKC) and cAMP/cGMP of cultured human pituitary adenoma cells.
  
  
  METHODS
  MTT colorimetry, (3)H-TdR and flow cytometry were used to detect the proliferation and radioimmunoassay was used to observe the effect of IL-2 on PKC activity and cAMP/cGMP levels of cultured human pituitary adenoma cells.
  
  
  RESULTS
  (1) IL-2 (1 x 10(4), 1 x 10(5) and 5 x 10(5) U/L) stimulated the proliferation and DNA synthesis of cultured human pituitary adenoma cells in a dose-dependent manner. (2) IL-2 (1 x 10(4), 1 x 10(5) and 5 x 10(5) U/L) decreased the ratio of pituitary adenoma cells in G(1) phase and increased the ratio in S and G(2) phase markedly. (3) Compared with control, PMA, a PKC activator, increased the activity of membrane and total PKC in human pituitary adenoma cells. However, after treatment with IL-2 (1 x 10(5) U/L), a significant increase of the activity of cytoplasmic, membrane and total PKC in human pituitary adenoma cells was observed. (4) IL-2 (5 x 10(4), 1 x 10(5) U/L) decreased the amount of cAMP in the cytoplasm of human pituitary adenoma cells, but had no effect on that of cGMP.
  
  
  CONCLUSIONS
  IL-2 can stimulate the proliferation of pituitary adenoma cells through PKC and cAMP/cGMP signaling pathways.
authors.affiliation: Department of Central Laboratory, Chengdu Military Command General Hospital, Chengdu 610083, China. zouziying49@163.com
authors.forename: Zi-ying
authors.initials: ZY
authors.lastname: Zou
authors.forename: Cheng-liang
authors.initials: CL
authors.lastname: Yuan
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.forename: Bi
authors.initials: B
authors.lastname: Zhao
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Hu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D010911:Pituitary Neoplasms / Q000188:drug therapy*; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D007376:Interleukin-2; D000242:Cyclic AMP; D011493:Protein Kinase C; D006152:Cyclic GMP