Purification and properties of a xylanase from Ceriporiopsis subvermispora cultivated on Pinus taeda.
The production of hemicellulose and cellulose degrading enzymes by the white-rot fungus Ceriporiopsis subvermispora was determined while growing in Pinus taeda wood chips. Enzymes produced by the fungus were extracted after 30 days of cultivation and at least two different xylanases were secreted. An endo-(1,4)-beta-xylanase was purified by means of ultrafiltration, anion exchange chromatography and gel filtration. Its molecular mass was 29 kDa and the pH and temperature optima were 5.0 and 60 degrees C, respectively. The endo-xylanase was able to hydrolyze xylan to principally xylotriose and xylotetraose and it has different activities against different xylans. With birchwood xylan as substrate, the enzyme showed a K(m) of 1.93 mg/ml and specific activity of 538 units/mg protein at 50 degrees C.
Milagres
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AM
Departamento de Biotecnologia, Faculdade de Engenharia Química de Lorena-Faenquil, Lorena-SP, CP 116 CEP 12 600 970, Brazil. adriane@debiq.faenquil.br
Magalhães
P O
PO
Ferraz
A
A
D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D041603:Pinus taeda / Q000382:microbiology; D020072:Polyporales / Q000201:enzymology*; D014995:Xylosidases / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*
D014995:Xylosidases