Post-transcriptional regulation in Xenopus embryos: role and targets of EDEN-BP.
EDEN (embryo deadenylation element)-dependent deadenylation is a regulatory process that was initially identified in Xenopus laevis early embryos and was subsequently shown to exist in Drosophila oocytes. Recent data showed that this regulatory process is required for somitic segmentation in Xenopus. Inactivation of EDEN-BP (EDEN-binding protein) causes severe segmentation defects, and the expression of segmentation markers in the Notch signalling pathway is disrupted. We showed that the mRNA encoding XSu(H) (Xenopus suppressor of hairless), a protein central to the Notch pathway, is regulated by EDEN-BP. Our data also indicate that other segmentation RNAs are targets for EDEN-BP. To identify new EDEN-BP targets, a microarray analysis has been undertaken.
Osborne
H B
HB
CNRS UMR 6061, IFR 140, Faculté de Médicine, Université de Rennes 1, Rennes, France. beverley.osborne@univ-rennes1.fr
Gautier-Courteille
C
C
Graindorge
A
A
Barreau
C
C
Audic
Y
Y
Thuret
R
R
Pollet
N
N
Paillard
L
L
D000818:Animals; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D012323:RNA Processing, Post-Transcriptional; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051880:Receptors, Notch / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D029867:Xenopus Proteins / Q000378:metabolism*; D014982:Xenopus laevis / Q000033:anatomy & histology / Q000196:embryology / Q000235:genetics
C110462:EDEN-specific RNA-binding protein, Xenopus; D016601:RNA-Binding Proteins; D051880:Receptors, Notch; D012097:Repressor Proteins; D029867:Xenopus Proteins