title: Patterning cultured cells by visible light illumination with photosensitizers.
abstract: We showed that PC12 cells and 3T3 cells cultured in dishes were killed by illumination with visible white light from a halogen lamp at 7 x 10(4) lx for 5 min in the presence of either 10 microM hematoporphyrin or 2 microM methylene blue as a photosensitizer. This simple technique, based on the photodynamic reaction via generation of reactive oxygen species can be applicable for patterning cultured cells.
authors.affiliation: Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Sakai, Osaka 599-8570, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Waki
authors.forename: Sumiko
authors.initials: S
authors.lastname: Gamo
authors.forename: Masahiko
authors.initials: M
authors.lastname: Bessho
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hara
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D006415:Hematoporphyrins / Q000494:pharmacology*; D008027:Light; D008751:Methylene Blue / Q000494:pharmacology*; D051379:Mice; D016716:PC12 Cells; D017319:Photosensitizing Agents / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D006415:Hematoporphyrins; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D008751:Methylene Blue