title: Isolation and characterization of a new extracellular bacteriolytic endopeptidase of Lysobacter sp. XL1.
abstract: The previously unstudied bacteriolytic enzyme L(4) was isolated from the culture liquid of the bacterium Lysobacter sp. XL1 in electrophoretically homogeneous state. The enzyme L(4) is a diaminopimelinoyl-alanine endopeptidase relative to peptidoglycan of Lysobacter sp. XL1. The enzyme is an alkaline protein of approximately 21 kD. The N-terminal amino acid sequence of the enzyme has been determined - A V V N G V N Y V Gx T T A ... The maximal activity of the enzyme was observed in 0.05 M Tris-HCl at pH 8.0 and 50-55 degrees C. The half-inactivation temperature of the enzyme is 52 degrees C. The endopeptidase L(4) is not a metalloenzyme since it is not affected by EDTA. The enzyme is inhibited by p-chloromercuribenzoic acid by 72% and by phenylmethylsulfonyl fluoride by 43%, which indicates the involvement of serine and thiol groups in its functioning.
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia. Stepnaya@ibpm.pushchino.ru
authors.forename: O A
authors.initials: OA
authors.lastname: Stepnaya
authors.forename: I M
authors.initials: IM
authors.lastname: Tsfasman
authors.forename: I A
authors.initials: IA
authors.lastname: Logvina
authors.forename: L P
authors.initials: LP
authors.lastname: Ryazanova
authors.forename: T A
authors.initials: TA
authors.lastname: Muranova
authors.forename: I S
authors.initials: IS
authors.lastname: Kulaev
mesh_terms: D000595:Amino Acid Sequence; D001433:Bacteriolysis / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000737:chemistry / Q000302:isolation & purification*; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D013696:Temperature; D044166:Xanthomonadaceae / Q000737:chemistry / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D010446:Peptide Fragments; D010450:Endopeptidases