title: On-probe sample pretreatment for direct analysis of lipids in gram-positive bacterial cells by matrix-assisted laser desorption ionization mass spectrometry.
abstract: On-probe sample pretreatment using trifluoroacetic acid as an additional reagent enabled the direct detection of phospholipids in whole bacteria by means of matrix-assisted laser desorption ionization mass spectrometry for not only gram-negative organisms but also gram-positive ones with a thicker peptidoglycan layer.
authors.affiliation: Division of Energy Science, EcoTopia Science Institute, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. ishida@esi.nagoya-u.ac.jp
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ishida
authors.forename: Kuniyuki
authors.initials: K
authors.lastname: Kitagawa
authors.forename: Akihito
authors.initials: A
authors.lastname: Nakayama
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohtani
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry / Q000166:cytology; D001431:Bacteriological Techniques; D006094:Gram-Positive Bacteria / Q000737:chemistry* / Q000166:cytology; D010743:Phospholipids / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014269:Trifluoroacetic Acid / Q000494:pharmacology
chemical_list: D010743:Phospholipids; D014269:Trifluoroacetic Acid