2-Chlorobenzoate biodegradation by recombinant Burkholderia cepacia under hypoxic conditions in a membrane bioreactor.
The feasibility of applying bacterial hemoglobin technology to degrade 2-chlorobenzoate (2-CBA) through co-metabolism under hypoxic conditions in a membrane bioreactor (MBR) process has been studied in the laboratory. 2-chlorobenzoate removal and chloride release rates in the MBR system varied from 99 to 78% and 98 to 73%, respectively, depending on the operation conditions. Chemical oxygen demand (COD) removal efficiencies were more than 90% at food-to-microorganism ratios ranging from 0.32 to 0.62 g/g/d, and the observed yield was 0.13 to 0.20 g biomass/g COD. The bacterial cell-floc size-distribution analysis showed that there is a significant change in bacterial floc size due to high shear stress during operation of the MBR. To characterize growth kinetics of Burkholderia cepacia strain dinitrotoluene, a mathematical model that describes co-metabolic oxidation of 2-CBA in an MBR has been developed.
Urgun-Demirtas
Meltem
M
Department of Chemical and Environmental Engineering, Illinois Institute of Technology, Chicago 60616, USA.
Stark
Benjamin
B
Pagilla
Krishna
K
D001673:Biodegradation, Environmental; D019149:Bioreactors; D016956:Burkholderia cepacia / Q000235:genetics / Q000378:metabolism*; D002712:Chlorides; D002723:Chlorobenzoates / Q000378:metabolism*; D017744:Medical Waste Disposal / Q000379:methods*; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods
D002712:Chlorides; D002723:Chlorobenzoates; D017744:Medical Waste Disposal; C060415:2-chlorobenzoic acid; D010100:Oxygen