Production of L-talitol from L-psicose by Metschnikowia koreensis LA1 isolated from soy sauce mash.
A strain LA1 that can convert L-psicose to L-talitol was isolated from soy sauce mash and identified as Metschnikowia koreensis. The cells grown on L-arabitol were found to have relatively high conversion potential. Addition of D-sorbitol to the reaction mixture considerably accelerated the conversion rate of L-psicose to L-talitol. During the conversion reaction, D-sorbitol was added to the reaction mixture at 12-h intervals to maintain the concentration of D-sorbitol at 1.0%. The final conversion ratios were 81.4%, 75.2%, 73.0%, 60.4% and 43.5% using washed cells when the concentrations of L-psicose were 0.5%, 1.0%, 2.0%, 3.0% and 5.0%, respectively. The product from L-psicose was identified as L-talitol by HPLC analysis, and infrared spectroscopy, optical rotation and melting point measurements.
Sasahara
Hiroyuki
H
Kagawa Prefectural Industrial Technology Center, Fermentation and Food Reserch Branch, Uchinomi-cho, Syozu-gun, Kagawa 761-4421, Japan.
Izumori
Ken
K
D001709:Biotechnology / Q000379:methods*; D005632:Fructose / Q000378:metabolism*; D004718:Saccharomycetales / Q000302:isolation & purification / Q000378:metabolism*; D013012:Sorbitol / Q000378:metabolism; D045730:Soy Foods / Q000382:microbiology*; D013402:Sugar Alcohols / Q000378:metabolism*
D013402:Sugar Alcohols; C003243:psicose; D005632:Fructose; D013012:Sorbitol; C042890:altritol; C014999:arabitol