title: [Tissue engineering of dentin-pulp complex-like structures by human dental mesenchymal cells].
abstract: OBJECTIVE
  To establish three-dimensional culture model of human dental mesenchymal cells and bioengineer in vivo with ceramic bovine bone (CBB) and Collagraft as scaffolds.
  
  
  METHODS
  Human dental mesenchymal cells induced upon stimulation of bFGF and IGF-1 or TGF-beta(1) were implanted onto CBB and Collagraft containing the same kinds of growth factors respectively. Then cell/scaffold constructs were transplanted into nude mice to establish in vivo culture model of dental mesenchymal cells. Control groups were set up at the same time. After 4 weeks or 10 weeks, the implants were taken out for histological and immunohistochemical analysis.
  
  
  RESULTS
  Within 10-week implant tissues, typical dentin-pulp complex-like structures were generated in scaffolds containing growth factors. Human dentin sialoprotein (DSP) was expressed in the newly formed dentin. This phenomenon wasn't observed in control groups and 4-week implants.
  
  
  CONCLUSIONS
  Dentin-pulp complex-like structures could be bioengineered successfully with human dental mesenchymal cells and CBB or Collagrafts containing growth factors in nude mice.
authors.affiliation: Shanghai Key Laboratory of Tissue Engineering, Affiliated Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
authors.forename: Liu-yu
authors.initials: LY
authors.lastname: Bao
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Jun-nan
authors.initials: JN
authors.lastname: Shi
authors.forename: Zhong-ying
authors.initials: ZY
authors.lastname: Niu
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Han-guo
authors.initials: HG
authors.lastname: Wang
mesh_terms: D000818:Animals; D002130:Calcium Phosphates; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen; D003782:Dental Pulp; D003804:Dentin; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D009805:Odontogenesis; D023822:Tissue Engineering / Q000379:methods*; D014094:Tooth, Deciduous / Q000166:cytology* / Q000196:embryology
chemical_list: D002130:Calcium Phosphates; C065819:Collagraft; D003094:Collagen