A reference map of the Arabidopsis thaliana mature pollen proteome.
The male gametophyte (or pollen) plays an obligatory role during sexual reproduction of higher plants. The extremely reduced complexity of this organ renders pollen a valuable experimental system for studying fundamental aspects of plant biology such as cell fate determination, cell-cell interactions, cell polarity, and tip-growth. Here, we present the first reference map of the mature pollen proteome of the dicotyledonous model plant species, Arabidopsis thaliana. Based on two-dimensional gel electrophoresis, matrix-assisted laser desorption/ionization time-of-flight, and electrospray quadrupole time-of-flight mass spectrometry, we reproducibly identified 121 different proteins in 145 individual spots. The presence, subcellular localization, and functional classification of the identified proteins are discussed in relation to the pollen transcriptome and the full protein complement encoded by the nuclear Arabidopsis genome.
Noir
Sandra
S
Max-Planck-Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829 Köln, Germany.
Bräutigam
Anne
A
Colby
Thomas
T
Schmidt
Jürgen
J
Panstruga
Ralph
R
D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D010455:Peptides / Q000378:metabolism; D011058:Pollen / Q000254:growth & development* / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
D029681:Arabidopsis Proteins; D010455:Peptides; D020033:Protein Isoforms; D020543:Proteome