title: Repair of Class II furcation defects after a reparative tissue graft obtained from extraction sockets treated with growth factors: a histologic and histometric study in dogs.
abstract: BACKGROUND
  Reparative tissue of extraction sockets was proposed as grafting material in the treatment of periodontal defects. Our hypothesis was that the addition of growth factors to extraction sockets improves the regenerative potential of this tissue when used as a graft. The objective of the present study was to analyze qualitatively and quantitatively the repair of acute Class II furcation defects after they receive this grafting material.
  
  
  METHODS
  The second and third upper premolars were extracted from four dogs. Platelet-derived growth factor (PDGF)-BB and insulin-like growth factor (IGF)-I, at concentrations of 6 microg/ml each, were applied to the resulting sockets. After 5 days, 24 acute defects (12 control and 12 test defects) were created in the second, third, and fourth lower premolars. Only the test sites received the graft. The flaps were positioned coronally on both sides and sutured. After 45 days, the specimens were collected, decalcified, and processed histologically in a buccal-lingual plane. The parameters were measured horizontally in the buccal-lingual direction.
  
  
  RESULTS
  Repair was histologically and histometrically similar in the two groups. No significant difference was observed between the test and control groups in the parameters connective tissue, new cementum, new bone, and junctional epithelium.
  
  
  CONCLUSIONS
  The use of this graft did not show beneficial effects on the repair of acute Class II furcation defects in dogs.
authors.affiliation: Discipline of Periodontics, Department of Stomatology, School of Dentistry, University of São Paulo, São Paulo, Brazil. fsoares@cos.odo.br
authors.forename: Fernando Peixoto
authors.initials: FP
authors.lastname: Soares
authors.forename: Fernando
authors.initials: F
authors.lastname: Hayashi
authors.forename: Christiane Watanabe
authors.initials: CW
authors.lastname: Yorioka
authors.forename: Claudio Mendes
authors.initials: CM
authors.lastname: Pannuti
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Gioso
authors.forename: Luiz Antonio Pugliesi Alves
authors.initials: LA
authors.lastname: de Lima
authors.forename: Giuseppe Alexandre
authors.initials: GA
authors.lastname: Romito
authors.forename: Francisco Emílio
authors.initials: FE
authors.lastname: Pustiglioni
mesh_terms: D000818:Animals; D000077214:Becaplermin; D004285:Dogs; D017823:Furcation Defects / Q000601:surgery*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D010519:Periodontium / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D011897:Random Allocation; D012038:Regeneration; D016378:Tissue Transplantation / Q000379:methods*; D020390:Tooth Socket / Q000187:drug effects / Q000601:surgery; D014945:Wound Healing / Q000187:drug effects
chemical_list: D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D007334:Insulin-Like Growth Factor I