title: [The molecular mechanism of PML in inducing apoptosis of bladder cancer cell line].
abstract: OBJECTIVE
  Study the affection of inducing expression of PML in the apoptosis and the molecular mechanism of bladder cancer.
  
  
  METHODS
  PMEP4/PML inducible expression vector was transfected into bladder cancer UM-UC-2 cells by lipofectamine 2000 system. The positive clone cells were selected by 300 microg/ml hygromycin B and confirmed by laser confocal imaging system. Then, using the in vitro DNA ladder apoptotic assay and Western blot, the affection of inducing expression of PML on apoptosis and its molecular mechanism of bladder cancer cell was studied.
  
  
  RESULTS
  Comparing with the vector control group, PML specific nuclear speckle significantly increased in the PMEP4/PML bladder cells. DNA ladder assay demonstrated bladder cancer cell expressing PML occurred apoptosis while the control vector cells were not influenced. Overexpression of PML could reduce Survivin expression and upregulate caspase3 and cleaved PARP protein expression
  
  
  CONCLUSIONS
  Our results demonstrated that overexpression of PML could induce bladder cancer cell apoptosis through the caspase dependent pathways.
authors.affiliation: Institute of Urology, No.1 Hospital, Xi'an Jiaotong University, Shanxi 710061, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Da-lin
authors.initials: DL
authors.lastname: He
authors.forename: Hui
authors.initials: H
authors.lastname: He
authors.forename: Xin-yang
authors.initials: XY
authors.lastname: Wang
authors.forename: Lin-lin
authors.initials: LL
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Xun-yi
authors.initials: XY
authors.lastname: Nan
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human