The Drosophila nucleosome remodeling factor NURF is required for Ecdysteroid signaling and metamorphosis.
Drosophila NURF is an ISWI-containing ATP-dependent chromatin remodeling complex that regulates transcription by catalyzing nucleosome sliding. To determine in vivo gene targets of NURF, we performed whole genome expression analysis on mutants lacking the NURF-specific subunit NURF301. Strikingly, a large set of ecdysone-responsive targets is included among several hundred NURF-regulated genes. Null Nurf301 mutants do not undergo larval to pupal metamorphosis, and also enhance dominant-negative mutations in ecdysone receptor. Moreover, purified NURF binds EcR in an ecdysone-dependent manner, suggesting it is a direct effector of nuclear receptor activity. The conservation of NURF in mammals has broad implications for steroid signaling.
Badenhorst
Paul
P
Laboratory of Molecular Cell Biology, National Cancer Institute, NIH, Bethesda, Maryland 20814, USA. p.w.badenhorst@bham.ac.uk
Xiao
Hua
H
Cherbas
Lucy
L
Kwon
So Yeon
SY
Voas
Matt
M
Rebay
Ilaria
I
Cherbas
Peter
P
Wu
Carl
C
D000818:Animals; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D026461:Ecdysteroids / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D007814:Larva / Q000502:physiology; D008322:Mammals / Q000235:genetics / Q000378:metabolism; D008675:Metamorphosis, Biological / Q000502:physiology*; D009154:Mutation; D009707:Nucleosomes / Q000378:metabolism*; D011987:Receptors, Steroid / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*
D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; C437989:E(bx) protein, Drosophila; D026461:Ecdysteroids; D009707:Nucleosomes; D011987:Receptors, Steroid; C055469:ecdysone receptor