title: Sentinel node biopsy for cT1 and cT2a gastric cancer.
abstract: OBJECTIVE
  To evaluate the feasibility and accuracy of sentinel node (SN) biopsy for gastric cancer.
  
  
  METHODS
  One hundred patients with gastric cancer diagnosed as cT1 (n=80) or cT2a (n=20) were enrolled. Indocyanine green-stained SNs were analysed by hematoxylin and eosin staining (n=100) and by cytokeratin immunohistochemistry (n=50).
  
  
  RESULTS
  SNs were identified in 94 of the 100 patients and the mean number of SNs was 4.4 (range, 1-12). Of these 94 patients, 14 patients had lymph node metastases. Two patients with T1 and one patient with T2 had metastases in non-SNs alone by hematoxylin and eosin staining (diagnostic accuracy =97.3% in T1 and 95.0% in T2). All three patients with a false negative result had a tumour, which was more than 4 cm in size and signet ring cell histology. In two of them, the tumour was located at lesser curvature. By immunohistochemical staining, three patients with T1 and one patient with T2 were found to have lymph node micrometastases in non-SNs alone among 45 patients (diagnostic accuracy =92.1% in T1, 85.7% in T2).
  
  
  CONCLUSIONS
  SN biopsy using indocyanine green can be performed rapidly and easily with a high detection rate and accuracy in patients with T1 gastric cancer. However, it should be performed with caution for large tumours with a signet ring cell histology located at lesser curvature due to the possibility of a false negative result.
authors.affiliation: Department of Surgery, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-744, South Korea.
authors.forename: D J
authors.initials: DJ
authors.lastname: Park
authors.forename: H-J
authors.initials: HJ
authors.lastname: Lee
authors.forename: H S
authors.initials: HS
authors.lastname: Lee
authors.forename: W H
authors.initials: WH
authors.lastname: Kim
authors.forename: H-H
authors.initials: HH
authors.lastname: Kim
authors.forename: K U
authors.initials: KU
authors.lastname: Lee
authors.forename: K J
authors.initials: KJ
authors.lastname: Choe
authors.forename: H-K
authors.initials: HK
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D004396:Coloring Agents; D003937:Diagnosis, Differential; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007208:Indocyanine Green; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D015203:Reproducibility of Results; D012189:Retrospective Studies; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D004396:Coloring Agents; D007208:Indocyanine Green