title: Evaluation of diagnostic tests for Trypanosoma evansi in experimentally infected pigs and subsequent use in field surveys in north Vietnam and Thailand.
abstract: This study is concerned with the evaluation of established diagnostic tests for diagnosis of Trypanosoma evansi in pigs. The immune trypanolysis test (TL), card agglutination test (CATT), latex agglutination test (LATEX), enzyme-linked immunosorbent assay (ELISA), microhaematocrit centrifugation technique (MHCT) and mouse inoculation (MI) tests were initially evaluated in experimentally infected fattening pigs. All infected pigs were confirmed parasitologically positive with both MHCT and MI. Results of the serological assays indicated that the TL could be a reference test for the presence of RoTat 1.2 antibodies in pigs. The results of the CATT and LATEX were inconsistent with the TL while the ELISA results correlated with the TL results. The four serological assays were subsequently used in two field surveys in Vietnam and Thailand. Results of the two agglutination assays (CATT and LATEX) were not consistent and did not correlate with TL results. The ELISA at percentage positivity of 22 appeared to have good ability to discriminate between seropositive and seronegative animals. Of the 437 samples collected at smallholder pig premises in northern Vietnam, no positive pigs were detected with the TL test. In Thailand, 77 samples were collected from five farrowing farms with a history of surra. Two parasitologically positive sows were found and on each farm seropositive sows were detected.
authors.affiliation: Ghent University, Faculty of Veterinary Medicine, Department of Virology, Parasitology and Immunology, Merelbeke, Belgium. wicher.holland@intervet.com
authors.forename: W G
authors.initials: WG
authors.lastname: Holland
authors.forename: N G
authors.initials: NG
authors.lastname: Thanh
authors.forename: T T
authors.initials: TT
authors.lastname: Do
authors.forename: S
authors.initials: S
authors.lastname: Sangmaneedet
authors.forename: B
authors.initials: B
authors.lastname: Goddeeris
authors.forename: J
authors.initials: J
authors.lastname: Vercruysse
mesh_terms: D000372:Agglutination Tests / Q000379:methods / Q000662:veterinary; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D006400:Hematocrit / Q000662:veterinary; D007841:Latex Fixation Tests / Q000379:methods / Q000662:veterinary; D008297:Male; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000592:standards / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology; D013785:Thailand; D014345:Trypanosoma / Q000276:immunology / Q000302:isolation & purification*; D014352:Trypanosomiasis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D014744:Vietnam
chemical_list: D000913:Antibodies, Protozoan