12-oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis.
Jasmonic acid (JA) and methyl jasmonate (MeJA), collectively known as JAs, regulate diverse physiological processes in plants, including the response to wounding. Recent reports suggest that a cyclopentenone precursor of JA, 12-oxo-phytodienoic acid (OPDA), can also induce gene expression. However, little is known about the physiological significance of OPDA-dependent gene expression. We used microarray analysis of approximately 21,500 Arabidopsis (Arabidopsis thaliana) genes to compare responses to JA, MeJA, and OPDA treatment. Although many genes responded identically to both OPDA and JAs, we identified a set of genes (OPDA-specific response genes [ORGs]) that specifically responded to OPDA but not to JAs. ORGs primarily encoded signaling components, transcription factors, and stress response-related genes. One-half of the ORGs were induced by wounding. Analysis using mutants deficient in the biosynthesis of JAs revealed that OPDA functions as a signaling molecule in the wounding response. Unlike signaling via JAs, OPDA signaling was CORONATINE INSENSITIVE 1 independent. These results indicate that an OPDA signaling pathway functions independently of JA/MeJA signaling and is required for the wounding response in Arabidopsis.
Taki
Nozomi
N
Department of Bioscience, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Sasaki-Sekimoto
Yuko
Y
Obayashi
Takeshi
T
Kikuta
Akihiro
A
Kobayashi
Koichi
K
Ainai
Takayuki
T
Yagi
Kaori
K
Sakurai
Nozomu
N
Suzuki
Hideyuki
H
Masuda
Tatsuru
T
Takamiya
Ken-Ichiro
K
Shibata
Daisuke
D
Kobayashi
Yuichi
Y
Ohta
Hiroyuki
H
D000171:Acrolein / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D015152:Blotting, Northern; D003517:Cyclopentanes / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics; D009154:Mutation / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins; D010935:Plant Diseases / Q000235:genetics*; D018515:Plant Leaves; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D054883:Oxylipins; D012333:RNA, Messenger; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; D000171:Acrolein