title: Specific induction of oxidative stress in terminal bronchiolar Clara cells during dimethylarsenic-induced lung tumor promoting process in mice.
abstract: The induction of oxidative stress in pulmonary cells during the process of lung tumor promotion by dimethylarsinic acid (DMA), a main metabolite of inorganic arsenics in mammals, was examined by immunohistochemical analysis using a specific antibody against 4-hydroxy-2-nonenal (4HNE) adducts, which are major aldehydic metabolites of lipid peroxidation. We demonstrated that 4HNE-modified proteins existed specifically in the secretory granules in terminal bronchiolar Clara cells. Furthermore, the degree of positive staining increased with the duration of DMA administration. Transmission electron microscopy revealed morphological changes in the Clara cells of DMA-treated mice. These results suggest that Clara cells are the major target cell for DMA-induced oxidative stress and that the cells may play an important role in the lung tumor promotion process in mice.
authors.affiliation: Department of Biochemical Toxicology, College of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi, Chiba 274-8555, Japan.
authors.forename: Yan
authors.initials: Y
authors.lastname: An
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Otsu
authors.forename: Shoji
authors.initials: S
authors.lastname: Okada
authors.forename: Kenzo
authors.initials: K
authors.lastname: Yamanaka
mesh_terms: D000818:Animals; D002101:Cacodylic Acid / Q000633:toxicity*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D006540:Herbicides / Q000633:toxicity*; D015227:Lipid Peroxidation; D008168:Lung / Q000166:cytology*; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000503:physiopathology; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress
chemical_list: D006540:Herbicides; D002101:Cacodylic Acid