title: [Effects of 17 beta-estradiol on the adhesion, invasion and motility potential of salivary mucoepidermoid carcinoma Mc3 cells].
abstract: OBJECTIVE
  To study the effects of 17 beta-estradiol on the adhesion, invasion and motility potential of salivary mucoepidermoid carcinoma Mc3 cells.
  
  
  METHODS
  The effects of 17 beta-estradiol on adhesion, invasion and motility potential of salivary mucoepidermoid carcinoma Mc3 cells were investigated with cell attachment assay on fibronectin (FN), wound assay, chemotaxis assay, and gelatin-incorporated SDS-PAGE electrophoresis. The expression of estrogen receptor in Mc3 cells was determined by immunohistochemistry assay.
  
  
  RESULTS
  Attachment rates of Mc3 cells treated with E2 at 10(-9), 10(-8), 10(-7), 10(-6) mol/L were 38.3%, 50.4%, 69.2% and 91.1% respectively, and the rate in control was 25.0%. When exposed to 17 beta-estradiol at 10(-9), 10(-8), 10(-7) and 10(-6) mol/L for 48 h, motility of Mc3 cells on FN increased by 16.9%, 40.9%, 36.4% and 38.8% respectively. When at 10(-6) mol/l, 17 beta-estradiol increased chemotaxis potential of Mc3 cells to FN by 60.3%. The activity of 68 000 matrix metalloproteinase (MMP-2) of Mc3 cells was enhanced at different levels by 10(-9), 10(-8), 10(-7), 10(-6) mol/L of 17 beta-estradiol, and estrogen receptor was also detected in nucleus of Mc3 cells by immunohistochemistry assay.
  
  
  CONCLUSIONS
  17 beta-estradiol at physiological concentration may enhance the adhesion, invasion and motility potential of salivary mucoepidermoid carcinoma Mc3 cells.
authors.affiliation: Department of Oral Biology, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: De-sheng
authors.initials: DS
authors.lastname: Wen
authors.forename: Jun-zheng
authors.initials: JZ
authors.lastname: Wu
authors.forename: Shan-min
authors.initials: SM
authors.lastname: Fu
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Li
authors.forename: Ji-jun
authors.initials: JJ
authors.lastname: Dong
mesh_terms: D018277:Carcinoma, Mucoepidermoid / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D004958:Estradiol / Q000494:pharmacology*; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D012468:Salivary Gland Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D004958:Estradiol