Comparing the sensitivity of anaplasma phagocytophilum DNA detection in Ixodes ricinus ticks by amplifying a fragment of the epank-1 and the 16s rDNA genes.
The study compared the sensitivity of two genetic markers (epank1, 16S rDNA) in identifying anaplasma DNA by using the polymerase chain reaction (PCR) technique. Material for anaplasma DNA studies was isolated from I. ricinus. Amplification of the 16S rDNA gene resulted in 5.77% positive samples, the value for the epank-1 gene equaled 11.54%. The results suggest that the sensitivity of the reaction for the epank-1 gene is greater than that of 16S rDNA for the identification of anaplasma DNA from isolates of I. ricinus. The application of epank- in identifying A. phagocytophilum DNA can change the pattern of infection in these arachnids in a given area. The results obtained in this study and cared out by another authors point at small useful a conservative gene as a marker for diagnostic goals.
Rymaszewska
Anna
A
Katedra Genetyki, Uniwersytet Szczeciński.
D041081:Anaplasma phagocytophilum / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004275:DNA, Ribosomal; D018884:Ixodes / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal