Proliferation and differentiation of rat bone marrow stromal cells on poly(glycolic acid)-collagen sponge.
We studied the effects of dexamethasone (Dex) and basic fibroblast growth factor (bFGF) on proliferation and differentiation of rat bone marrow stromal cells (RBMSCs), using three scaffolds: collagen sponge, poly(glycolic acid) (PGA)-collagen sponge, and PGA-collagen (UV) sponge. RBMSCs were seeded into the sponges, and cultured in primary medium, primary medium with Dex, and primary medium with bFGF and Dex. Three weeks after cultivation, we examined alkaline phosphatase (ALP) activity and cell number in the sponges, and also performed macroscopic, light microscopic, and scanning electron microscopic (SEM) observations. Collagen sponge shrank considerably, but PGA-collagen and PGA-collagen (UV) sponges maintained most of their original shape. PGA-collagen (UV) sponge supplemented with bFGF and Dex together had the highest ALP activity and cell number, followed by PGA-collagen sponge. Although collagen sponge showed cell proliferation only on the surface, the other two sponges showed cell proliferation in the interior. SEM showed the best cell attachment to PGA-collagen (UV) sponge in the presence of bFGF and Dex, followed by PGA-collagen sponge. In conclusion, PGA-collagen (UV) and PGA-collagen sponges proved to be much more useful as scaffolding for bone regeneration when combined with bFGF and Dex.
Fujita
Motoki
M
Department of Pathology, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Kinoshita
Yukihiko
Y
Sato
Emiko
E
Maeda
Hatsuhiko
H
Ozono
Satoshi
S
Negishi
Hideyuki
H
Kawase
Toshio
T
Hiraoka
Yosuke
Y
Takamoto
Tomoaki
T
Tabata
Yasuhiko
Y
Kameyama
Yoichiro
Y
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology; D001861:Bone Regeneration; D002448:Cell Adhesion; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000528:radiation effects; D024042:Collagen Type I / Q000737:chemistry / Q000528:radiation effects / Q000648:ultrastructure; D003907:Dexamethasone / Q000494:pharmacology; D004338:Drug Combinations; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D008297:Male; D008855:Microscopy, Electron, Scanning; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000528:radiation effects; D051381:Rats; D011916:Rats, Inbred F344; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D013552:Swine; D013710:Tendons / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*; D014466:Ultraviolet Rays
D000893:Anti-Inflammatory Agents; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D004338:Drug Combinations; D016222:Fibroblast Growth Factor 2; D011100:Polyglycolic Acid; D003907:Dexamethasone; D000469:Alkaline Phosphatase