Analysis of allele fidelity, polymorphic information content, and density of microsatellites in a genome-wide screening for hip dysplasia in a crossbreed pedigree.
Recent advances in genomics resources and tools are facilitating quantitative trait locus mapping. We developed a crossbreed pedigree for mapping quantitative trait loci for hip dysplasia in dogs by crossing dysplastic Labrador Retrievers and normal Greyhounds. We show that one advantage to using a crossbreed pedigree is the increased marker informativeness in the backcross/F2 population relative to the founder populations. We also discuss three factors that affect the detection power in the context of this crossbreed pedigree: being able to detect and correct genotyping errors, increasing marker density for chromosomes with a sparse coverage, and adding individuals to the mapping population as soon as they become available.
Mateescu
R G
RG
Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, NY 14853, USA.
Zhang
Z
Z
Tsai
K
K
Phavaphutanon
J
J
Burton-Wurster
N I
NI
Lust
G
G
Quaas
R
R
Murphy
K
K
Acland
G M
GM
Todhunter
R J
RJ
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D004285:Dogs; D005260:Female; D006619:Hip Dysplasia, Canine / Q000235:genetics*; D006824:Hybridization, Genetic; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010375:Pedigree; D011110:Polymorphism, Genetic / Q000235:genetics*; D015203:Reproducibility of Results; D012107:Research Design; D013223:Statistics as Topic / Q000379:methods