Neurite extension of DRG neurons by gicerin expression is enhanced by nerve growth factor.
Gicerin, a cell adhesion molecule, is expressed in dorsal root ganglion (DRG) and sciatic nerves during chick development. This molecule re-appears in these tissues after an injury to the sciatic nerve. In the present study, we investigated the expression of nerve growth factor (NGF) in the regenerating sciatic nerve of chicks and the effects of NGF on the expression and neurite activities of gicerin in DRG. In the sciatic nerve after a crush injury, the expression of NGF and gicerin increased in the Schwann cells and in the nerve fibers, respectively. NGF promoted the neurite projections from in vitro DRG on the gicerin ligands, which were inhibited by anti-NGF antibody. The gicerin mRNA expression increased in the DRG with NGF, which was inhibited by the co-incubation with anti-NGF antibody. These results indicate that NGF might therefore enhance the expression of gicerin in DRG, thereby promoting the gicerin-dependent neurite extension during sciatic nerve regeneration.
Hiroi
Satoshi
S
Department of Integrated Structural Biosciences, Veterinary School, Osaka Prefecture University, Osaka 599-8531, Japan.
Taira
Eiichi
E
Ogawa
Kazushige
K
Tsukamoto
Yasuhiro
Y
D000818:Animals; D051929:CD146 Antigen / Q000235:genetics / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology*; D015870:Gene Expression / Q000187:drug effects; D066298:In Vitro Techniques; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D009416:Nerve Regeneration; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012584:Sciatic Nerve / Q000166:cytology / Q000502:physiology
D051929:CD146 Antigen; D012333:RNA, Messenger; D020932:Nerve Growth Factor