Simple sequence repeat-based consensus linkage map of Bombyx mori.
We established a genetic linkage map employing 518 simple sequence repeat (SSR, or microsatellite) markers for Bombyx mori (silkworm), the economically and culturally important lepidopteran insect, as part of an international genomics program. A survey of six representative silkworm strains using 2,500 (CA)n- and (CT)n-based SSR markers revealed 17-24% polymorphism, indicating a high degree of homozygosity resulting from a long history of inbreeding. Twenty-nine SSR linkage groups were established in well characterized Dazao and C108 strains based on genotyping of 189 backcross progeny derived from an F(1) male mated with a C108 female. The clustering was further focused to 28 groups by genotyping 22 backcross progeny derived from an F(1) female mated with a C108 male. This set of SSR linkage groups was further assigned to the 28 chromosomes (established linkage groups) of silkworm aided by visible mutations and cleaved amplified polymorphic sequence markers developed from previously mapped genes, cDNA sequences, and cloned random amplified polymorphic DNAs. By integrating a visible mutation p (plain, larval marking) and 29 well conserved genes of insects onto this SSR-based linkage map, a second generation consensus silkworm genetic map with a range of 7-40 markers per linkage group and a total map length of approximately 3431.9 cM was constructed and its high efficiency for genotyping and potential application for synteny studies of Lepidoptera and other insects was demonstrated.
Miao
Xue-Xia
XX
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.
Xub
Shi-Jie
SJ
Li
Ming-Hui
MH
Li
Mu-Wang
MW
Huang
Jian-Hua
JH
Dai
Fang-Yin
FY
Marino
Susan W
SW
Mills
David R
DR
Zeng
Peiyu
P
Mita
Kazuei
K
Jia
Shi-Hai
SH
Zhang
Yong
Y
Liu
Wen-Bin
WB
Xiang
Hui
H
Guo
Qiu-Hong
QH
Xu
An-Ying
AY
Kong
Xiang-Yin
XY
Lin
Hong-Xuan
HX
Shi
Yao-Zhou
YZ
Lu
Gang
G
Zhang
Xianglin
X
Huang
Wei
W
Yasukochi
Yuji
Y
Sugasaki
Toshiyuki
T
Shimada
Toru
T
Nagaraju
Javaregowda
J
Xiang
Zhong-Huai
ZH
Wang
Sheng-Yue
SY
Goldsmith
Marian R
MR
Lu
Cheng
C
Zhao
Guo-Ping
GP
Huang
Yong-Ping
YP
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D002874:Chromosome Mapping; D008040:Genetic Linkage; D005819:Genetic Markers; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
D005819:Genetic Markers