[Role of interleukin-1 receptor antagonist in protecting kidney from injury induced by asphyxia in neonatal rats].
OBJECTIVE
To observe the role of recombinant human interleukin-1 receptor antagonist (rhIL-1ra) in protecting kidney from injury induced by asphyxia in neonatal rats.


METHODS
Neonatal rats were used as experimental animals. The changes in intrarenal inflammatory response and renal injury were examined in the control group (n=13), and 2, 24 and 48 hours after asphyxia followed by normal saline treatment in those treated with rhIL-1ra.


RESULTS
In normal saline group, the white blood cell count, the blood interleukin-1 (IL-1), IL-8, IL-6, nitric oxide (NO), endothelin (ET-1) levels, and the renal coefficient (LRC), the scores of injured tubules of the left kidney were significantly increased at 2 hours (n=10), 24 hours (n=11), and 48 hours (n=10, P<0.05 or P<0.01). Compared with the normal saline group, the levels of the above parameters, except IL-6, were significantly decreased in rhIL-1ra treatment group at the same time points (P<0.05 or P<0.01). Serum IL-6 at 24 hours and 48 hours was also decreased in rhIL-1ra treatment group significantly (P<0.05 or P<0.01).


CONCLUSIONS
The results suggest that rhIL-1ra may protect renal injury after asphyxia via inhibiting intrarenal inflammatory response.
Dong
Wen-bin
WB
Department of Pediatrics, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan, China.
Chen
Hong-ying
HY
Chen
Shu-qin
SQ
Long
Han-an
HA
Chen
Yue
Y
Qu
Xue-song
XS
Zou
Yan
Y
Tang
Zhang-hua
ZH
Deng
Cun-liang
CL
Hua
Li
L
D000818:Animals; D001237:Asphyxia / Q000188:drug therapy / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D053590:Interleukin 1 Receptor Antagonist Protein / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D053590:Interleukin 1 Receptor Antagonist Protein