title: Tolerance induction through simultaneous double bone marrow transplantation with two-signal blockade.
abstract: Cotransplantation of donor bone marrow cells (BMCs) in allograft recipients is currently the most promising concept for clinical tolerance induction; however, it still has many difficulties in its successful performance due to the toxicity of the required host conditioning, the risk of engraftment failure, and the problem of graft-versus-host disease (GVHD), as well as the limited accessibility of donor bone marrow cells. Therefore, we performed the studies to determine whether BMCs from multi-donors are simultaneously engrafted and lead to induction of chimerism-based tolerance through the tolerogenic protocol of whom effectiveness we have shown in a previous study. Using a murine model, it was demonstrated that grafted BMCs from BALB/c (H-2(d)) and CBA mice (H-2(k)) establish mixed type and multi-lineage double chimerism and induce immunological donor-specific tolerance to fully MHC-mismatched skin allografts in host C57BL/6 mice (H-2(b)) receiving conditioning with Busulfan and treatment with the two-signal blockade comprised of anti-CD45RB and anti-CD154 monoclonal antibodies.
authors.affiliation: Transplantation Research Center, Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Kangnam-Ku, Seoul 135-710, Republic of Korea.
authors.forename: Eun-Na
authors.initials: EN
authors.lastname: Lee
authors.forename: Jienny
authors.initials: J
authors.lastname: Lee
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Hae-Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Chi-Young
authors.initials: CY
authors.lastname: Chang
authors.forename: Da-Yeon
authors.initials: DY
authors.lastname: Jung
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Suk-Koo
authors.initials: SK
authors.lastname: Lee
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Joh
authors.forename: Choon-Hyuck
authors.initials: CH
authors.lastname: Kwon
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Noh
authors.forename: Sung-Joo
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000276:immunology* / Q000379:methods*; D005802:Genes, MHC Class II; D006085:Graft Survival; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016038:Skin Transplantation / Q000276:immunology*; D018183:Transplantation Chimera; D023001:Transplantation Tolerance; D014184:Transplantation, Homologous