Amperometric, screen-printed, glucose biosensor for analysis of human plasma samples using a biocomposite water-based carbon ink incorporating glucose oxidase.
This paper describes the optimisation of a screen-printing water-based carbon ink containing cobalt phthalocyanine (CoPC) and glucose oxidase (GOD) for the fabrication of a glucose biosensor. To optimise the performance of the biosensor, the loadings of the electrocatalyst (CoPC) and enzyme (GOD) were varied. It was found that the maximum linear range was achieved with a CoPC loading of 20% (m/m, relative to the mass of carbon) and a GOD loading of 628 U per gram of carbon. In our studies we chose to employ chronoamperometry, as this technique is commonly used for commercial devices. The optimum operating applied potential was found to be +0.5 V, following an incubation period of 60 s. The optimum supporting electrolyte was found to be 0.05 M phosphate buffer at pH 8.0, which resulted in a linear range of 0.2-5 mM, the former represents the detection limit. The sensitivity was 1.12 microA mM(-1). The effect of temperature was also investigated, and it was found that 40 degrees C gave optimal performance. The resulting amperometric biosensors were evaluated by measuring the glucose concentrations for 10 different human plasma samples containing endogenous glucose and also added glucose. The same samples were analysed by a standard spectrophotometric method, and the results obtained by the two different methods were compared. A good correlation coefficient (R(2) = 0.95) and slope (0.98) were calculated from the experimental data, indicating that the new devices hold promise for biomedical studies.
Crouch
Eric
E
Centre for Research in Analytical, Materials, and Sensors Science, Faculty of Applied Sciences, University of the West of England, Bristol, UK.
Cowell
David C
DC
Hoskins
Stephen
S
Pittson
Robin W
RW
Hart
John P
JP
D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D002244:Carbon / Q000737:chemistry*; D002384:Catalysis; D005949:Glucose Oxidase / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry; D007281:Ink; D009942:Organometallic Compounds / Q000737:chemistry; D011199:Potentiometry / Q000379:methods; D044967:Serum / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000737:chemistry
D001786:Blood Glucose; D007211:Indoles; D009942:Organometallic Compounds; D014867:Water; C063633:cobalt phthalocyanine; D002244:Carbon; D005949:Glucose Oxidase