Noninvasive measures of body fat percentage in male Yucatan swine.
The purpose of this study was to assess the use of body circumference, ultrasonography, and serum leptin levels as noninvasive measures to estimate body fat percentage in adult, male, Yucatan swine, which are widely used in biomedical research models. Swine (ages 8 to 15 months) were maintained for 20 weeks: control (n = 7); high-fat, high-cholesterol diet (hyperlipidemic; n = 8); alloxan-induced diabetes with high-fat, high-cholesterol diet (diabetic dyslipidemic; n = 7); and diabetic dyslipidemic plus exercise-trained (n = 6). Anesthetized swine were positioned on their dorsum for the following measurements: 1) neck, mid-abdomen, and widest abdominal girth circumferences; and 2) neck and mid-abdomen ultrasound measurements. Blood samples were obtained for quantification of serum leptin levels. After euthanasia, the carcass and viscera were separated for chemical composition analysis, which demonstrated a significant increase in carcass and visceral fat in the diabetic dyslipidemic swine compared to controls. Serum leptin levels were also increased in the hyperlipidemic and diabetic dyslipidemic swine. Regression analyses demonstrated a significant correlation between carcass fat, visceral fat, and all of the circumference, ultrasound, and serum leptin measures. In conclusion, the widest abdominal girth circumference was the noninvasive measure with the highest predictive value for estimating carcass and visceral fat in adult, male Yucatan miniature swine.
Witczak
Carol A
CA
Department of Medical Pharmacology & Physiology, School of Medicine, University of Missouri, Columbia, Missouri, USA.
Mokelke
Eric A
EA
Boullion
Robert
R
Wenzel
James
J
Keisler
Duane H
DH
Sturek
Michael
M
D000818:Animals; D001823:Body Composition; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000473:pathology; D004195:Disease Models, Animal; D050171:Dyslipidemias / Q000150:complications / Q000473:pathology; D050152:Intra-Abdominal Fat; D020738:Leptin / Q000097:blood; D008297:Male; D051379:Mice; D010805:Physical Conditioning, Animal; D050151:Subcutaneous Fat; D013552:Swine; D013556:Swine, Miniature
D020738:Leptin