title: Increased oxidative stress associated with the severity of the liver disease in various forms of hepatitis B virus infection.
abstract: BACKGROUND
  Oxidative stress can be defined as an increase in oxidants and/or a decrease in antioxidant capacity. There is limited information about the oxidative status in subjects with hepatitis B virus infection. We aimed to evaluate the oxidative status in patients with various clinical forms of chronic hepatitis B infection.
  
  
  METHODS
  Seventy-six patients with hepatitis B virus infection, in whom 33 with chronic hepatitis, 31 inactive carriers and 12 with cirrhosis, and 16 healthy subjects were enrolled. Total antioxidant response and total peroxide level measurement, and calculation of oxidative stress index were performed in all participants.
  
  
  RESULTS
  Total antioxidant response was significantly lower in cirrhotics than inactive HbsAg carriers and controls (p = 0.008 and p = 0.008, respectively). Total peroxide level and oxidative stress index was significantly higher in cirrhotic (p < 0.001, both) and chronic hepatitis B subjects (p < 0.001, both) than inactive HbsAg carriers and controls. Total antioxidant response was comparable in chronic hepatitis B subjects, inactive HbsAg carriers and controls (both, p > 0.05/6). Total peroxide level and oxidative stress index were also comparable in inactive HBsAg carriers and controls (both, p > 0.05/6). Serum alanine amino transferase level was positively correlated with total peroxide level and oxidative stress index only in chronic hepatitis B subjects (p = 0.002, r = 0.519 and p = 0.008, r = 0.453, respectively).
  
  
  CONCLUSIONS
  Oxidative stress occurs secondarily to increased total lipid peroxidation and inadequate total antioxidant response and is related to severity of the disease and replication status of virus in hepatitis B infection.
authors.affiliation: Gastroenterology Division, Department of Internal Medicine, Harran University, Medical Faculty, Sanliurfa, Turkey. drcengizbolukbas@hotmail.com
authors.forename: Cengiz
authors.initials: C
authors.lastname: Bolukbas
authors.forename: Fusun Filiz
authors.initials: FF
authors.lastname: Bolukbas
authors.forename: Mehmet
authors.initials: M
authors.lastname: Horoz
authors.forename: Mehmet
authors.initials: M
authors.lastname: Aslan
authors.forename: Hakim
authors.initials: H
authors.lastname: Celik
authors.forename: Ozcan
authors.initials: O
authors.lastname: Erel
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D000975:Antioxidants / Q000378:metabolism; D002353:Carrier State / Q000503:physiopathology; D005260:Female; D019694:Hepatitis B, Chronic / Q000378:metabolism* / Q000503:physiopathology*; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008103:Liver Cirrhosis / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress
chemical_list: D000975:Antioxidants; D000410:Alanine Transaminase