Performance of a T-cell-based diagnostic test for tuberculosis infection in HIV-infected individuals is independent of CD4 cell count.
The performance characteristics of the enzyme-linked immunospot assay (ELISPOT) assay (T-SPOT TB) for the diagnosis of latent tuberculosis infection in HIV-infected individuals are unknown. Given that ELISPOT enumerates Mycobacterium tuberculosis antigen-specific IFN-gamma-secreting T cells, HIV-associated immunosuppression might adversely affect test performance. However, we found that 28 out of 29 HIV-positive individuals (97%) gave evaluable test results, and performance was independent of the CD4 T-cell count. ELISPOT test performance appears to be independent of HIV-associated immunosuppression.
Dheda
Keertan
K
Centre for Infectious Diseases and International Health, Department of Population Sciences and Primary Care, Royal Free and University College Medical School, University College London, London, UK.
Lalvani
Ajit
A
Miller
Robert F
RF
Scott
Geoff
G
Booth
Helen
H
Johnson
Margaret A
MA
Zumla
Alimuddin
A
Rook
Graham Aw
GA
D000328:Adult; D017252:CD3 Complex / Q000276:immunology; D018791:CD4 Lymphocyte Count; D003955:Diagnostic Tests, Routine / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D015658:HIV Infections / Q000150:complications* / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D018655:Lymphocyte Count; D008297:Male; D010835:Phytohemagglutinins / Q000276:immunology; D012333:RNA, Messenger / Q000097:blood; D012367:RNA, Viral / Q000097:blood; D013601:T-Lymphocytes / Q000276:immunology*; D014376:Tuberculosis / Q000175:diagnosis* / Q000276:immunology
D017252:CD3 Complex; D007376:Interleukin-2; D010835:Phytohemagglutinins; D012333:RNA, Messenger; D012367:RNA, Viral; D015847:Interleukin-4; D007371:Interferon-gamma