Use of recombinant AdSV40-BetaGal adenoviral construction for monitoring of transplanted cells.
Original recombinant adenoviral construction carrying E. coli beta-galactosidase LacZ gene designed by the authors is convenient for labeling and monitoring of bone marrow mesenchymal (stromal) progenitor cells and myocardial and skin fetal cells transplanted in damaged rat tissues (in the perinecrotic zone of the myocardium and onto burnt skin surface) for their reparation. This genetic construction after pre-inactivation of endogenous beta-galactosidase allows to detect transplanted cells in the foci of injury; positive effects of transplantation on tissue reparation processes can be attributed to the presence of transplanted cells.
Dolgikh
M S
MS
Institute of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Moscow.
Grigor'eva
A Yu
AY
Zhigulin
A O
AO
Zaidenov
V A
VA
Rasulov
M F
MF
Potapov
I V
IV
Krasheninnikov
M E
ME
Onishchenko
N A
NA
D000256:Adenoviridae; D000818:Animals; D004926:Escherichia coli; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D006652:Histological Techniques; D007763:Lac Operon / Q000235:genetics; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase