Development of acrylate-based monolithic stationary phases for electrochromatographic separations.
Organic monolithic stationary phases were synthesized in fused-silica capillaries. They were prepared by in situ polymerization under UV irradiation of various alkyl acrylates, 1,3-butanediol diacrylate, and 2-acrylamido-2-methyl-1-propanesulfonic acid in a ternary porogenic solvent. The resulting stationary phases were tested in CEC. The influence of UV irradiation energy on the resulting separative performances of the monoliths was studied. It was thus demonstrated that the use of hexyl acrylate rather than butyl acrylate and lauryl methacrylate gives highly efficient monoliths (more than 300 000 plates per meter) with optimized EOF. It was also confirmed that the mobile phase ionic strength may affect significantly the separation efficiency. The influence of the nature of the mobile phase organic modifier (ACN or methanol) on EOF, retention, efficiency, and selectivity was studied and differences were observed. Finally, the performances of monolithic stationary phases developed and optimized for CEC separations were evaluated in nanoLC.
Barrioulet
Marie-Pierre
MP
Laboratoire des Sciences Analytiques, Université Claude Bernard Lyon I, Villeurbanne, France.
Delaunay-Bertoncini
Nathalie
N
Demesmay
Claire
C
Rocca
Jean-Louis
JL
D000179:Acrylates / Q000737:chemistry; D000180:Acrylic Resins / Q000138:chemical synthesis*; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D009994:Osmolar Concentration; D014466:Ultraviolet Rays
D000179:Acrylates; D000180:Acrylic Resins