Cytotoxicity of liposomal alpha-tocopheryl succinate towards hamster cheek pouch carcinoma (HCPC-1) cells in culture.
There is compelling evidence for the cancer chemopreventive effects of vitamin E and related compounds. Of all the vitamin E derivatives that have been investigated to date, vitamin E acid succinate is the most effective anti-cancer agent. This report describes the preparation and testing of liposomal formulation of mono alpha-tocopheryl ester of succinic acid (alpha-TOS) for cytotoxicity against hamster cheek pouch carcinoma cell line (HCPC-1). Small unilamellar vesicles (SUV) of phosphatidylcholine incorporating 70 microM alpha-TOS were superior to alpha-TOS alone or SUV without incorporated alpha-TOS, as inducers of apoptosis in HCPC-1 cells. Liposomal alpha-TOS perturbed the lipid structure in cells, promoted apoptosis, and decreased cell viability. The mechanism of action of alpha-TOS appears to involve membrane damage and induction of ceramide mediated apoptosis.
Gu
Xinbin
X
Department of Oral Diagnosis, College of Dentistry, Howard University, 600 W Street NW, Washington, DC 20059, USA. xgu@howard.edu
Schwartz
Joel L
JL
Pang
Xiaowu
X
Zhou
Yanfei
Y
Sirois
David A
DA
Sridhar
Rajagopalan
R
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002610:Cheek / Q000473:pathology*; D006224:Cricetinae; D005434:Flow Cytometry; D020287:In Situ Nick-End Labeling; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000473:pathology; D024505:Tocopherols; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use
D014810:Vitamin E; D024505:Tocopherols