The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol.
Cellular ion homeostasis involves communication between the cytosol and the luminal compartment of organelles. This is particularly critical for metal ions because of their toxic potential. We have identified the yeast homologue of the prokaryotic ArsA protein, the homodimeric ATPase Arr4p, as a protein that binds to the yeast intracellular CLC chloride-transport protein, Gef1p. We show that binding of Arr4p to the C terminus of Gef1p requires the presence of yeast cytosol and is sensitive to a highly specific copper chelator in vitro and in vivo. Copper alone can substitute for cytosol to support the interaction of Arr4p with the C terminus of Gef1p. The migration behavior of Arr4p in nonreducing gel electrophoresis correlates with cellular copper deficiency, repletion, or stress. Our homology model of Arr4p shows that the antimony (arsenic) metal binding site of ArsA is not conserved in Arr4p. The model suggests that a pair of cysteines, Cys285 and Cys288, is located in the interface of the Arr4p dimer. These residues are required for Arr4p homodimerization and for binding to the C terminus of Gef1p. Whereas both proteins are required for normal growth under iron-limiting conditions, they play opposite roles when copper and heat stress are combined in an alkaline environment. Under these conditions, deltagef1 cells grow much better than wild type yeast, whereas deltaarr4 cells are unable to grow. Comparison of the deltaarr4 with the deltaarr4deltagef1 strain suggests that Arr4p antagonizes the function of Gef1p.
Metz
Jutta
J
Zentrum für Molekulare Biologie der Universität Heidelberg, Im Neuenheimer Feld 282, Heidelberg D-69120, Germany.
Wächter
Andrea
A
Schmidt
Bastian
B
Bujnicki
Janusz M
JM
Schwappach
Blanche
B
D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000906:Antibodies; D053502:Arsenate Reductases; D053501:Arsenite Transporting ATPases; D018118:Chloride Channels / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D019281:Dimerization; D006168:Guinea Pigs; D018869:Heat-Shock Response; D016623:Ion Pumps; D009097:Multienzyme Complexes; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
D000906:Antibodies; D018118:Chloride Channels; D002712:Chlorides; C089713:GEF1 protein, S cerevisiae; D016623:Ion Pumps; D009097:Multienzyme Complexes; D029701:Saccharomyces cerevisiae Proteins; D003300:Copper; C506971:ARR2 protein, S cerevisiae; D053502:Arsenate Reductases; D000251:Adenosine Triphosphatases; D053501:Arsenite Transporting ATPases