title: [Generation of chemical-inducible activation tagging T-DNA insertion lines of Arabidopsis thaliana].
abstract: Using an estrogen-inducible expression XVE (LexA-VP16-Estragon Receptor) system, we have generated approximately 40 000 independent T-DNA insertion lines of Arabidopsis thaliana. Segregation analyses of about 18000 lines indicated that 51.6% of them contain single T-DNA insertions and that the average insertion number is 1.38 copies per line. Mutants displaying a variety of morphological alterations were identified, including those that affect development of roots,hypocotyls, leaves, floral organs and seeds as well as the flowering time.
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Jin-Xiang
authors.initials: JX
authors.lastname: Xu
authors.forename: Ying-Zhen
authors.initials: YZ
authors.lastname: Kong
authors.forename: Zhen-Dong
authors.initials: ZD
authors.lastname: Ji
authors.forename: Xing-Chun
authors.initials: XC
authors.lastname: Wang
authors.forename: Feng-Ying
authors.initials: FY
authors.lastname: An
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Jia-Qiang
authors.initials: JQ
authors.lastname: Sun
authors.forename: Su-Zhi
authors.initials: SZ
authors.lastname: Zhang
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Yang
authors.forename: Jin-Ye
authors.initials: JY
authors.lastname: Mu
authors.forename: Xin-Fang
authors.initials: XF
authors.lastname: Liu
authors.forename: Jia-Yang
authors.initials: JY
authors.lastname: Li
authors.forename: Yong-Biao
authors.initials: YB
authors.lastname: Xue
authors.forename: Jian-Ru
authors.initials: JR
authors.lastname: Zuo
mesh_terms: D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D004967:Estrogens / Q000494:pharmacology; D005822:Genetic Vectors / Q000235:genetics; D017405:Herpes Simplex Virus Protein Vmw65 / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000187:drug effects / Q000379:methods*; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics; D015533:Transcriptional Activation / Q000187:drug effects; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004967:Estrogens; D017405:Herpes Simplex Virus Protein Vmw65; D011960:Receptors, Estrogen; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein; C036483:T-DNA