Application of measurements of transepithelial electrical resistance of intestinal epithelial cell monolayers to evaluate probiotic activity.
Among five potentially probiotic lactobacilli investigated, Lactobacillus plantarum MF1298 and Lactobacillus salivarius DC5 showed the highest increase in the transepithelial electrical resistance (TER) of polarized monolayers of Caco-2 cells, and this increase was shown to be dose dependent. Furthermore, preincubation with MF1298 attenuated a decrease in TER induced by Listeria monocytogenes.
Klingberg
Trine Danø
TD
The Royal Veterinary and Agricultural University, Department of Food Science, Rolighedsvej 30, 4th Floor, DK-1958 Frederiksberg C, Denmark. tdk@kvl.dk
Pedersen
Maja Herold
MH
Cencic
Avrelija
A
Budde
Birgitte Bjørn
BB
D018938:Caco-2 Cells; D016764:Cell Polarity; D017097:Electric Impedance; D004847:Epithelial Cells / Q000382:microbiology / Q000502:physiology*; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000382:microbiology*; D007778:Lactobacillus / Q000254:growth & development / Q000502:physiology*; D008089:Listeria monocytogenes / Q000472:pathogenicity*; D019936:Probiotics / Q000378:metabolism*