title: Fumonisin production by Fusarium verticillioides strains isolated from maize in Mexico and development of a polymerase chain reaction to detect potential toxigenic strains in grains.
abstract: Fumonisins are mycotoxins produced by Fusarium verticillioides (Sacc. Nirenberg) in maize (Zea mays L.), a staple crop in Mexico. In this study, we report the isolation and identification of 67 Fusarium strains isolated from maize kernels collected in Northwest and Central Mexico. The strains were characterized regarding fumonisin B(1) production and the presence of the FUM1 gene. F. verticillioides was the predominant species isolated in both geographic regions, but the isolates from Northwest Mexico produced higher levels of fumonisin. A polymerase chain reaction (PCR)-based method, to detect a region of the FUM1 gene involved in fumonisin biosynthesis, was developed and employed to detect mycotoxigenic fungi in pure culture and in contaminated maize. The presence of the FUM1 gene was associated with fumonisin production in most isolates, except seven that did not synthesize fumonisin but contained the gene in their genome. The PCR method allowed the direct detection of fungal contamination in ground corn and could be employed to screen for the presence of potential mycotoxigenic fusaria.
authors.affiliation: Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México Av. Universidad y Copilco, 04510 México, D.F., México.
authors.forename: Diana
authors.initials: D
authors.lastname: Sánchez-Rangel
authors.forename: Andrea
authors.initials: A
authors.lastname: SanJuan-Badillo
authors.forename: Javier
authors.initials: J
authors.lastname: Plasencia
mesh_terms: D001483:Base Sequence; D004271:DNA, Fungal / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D037341:Fumonisins / Q000378:metabolism*; D005670:Fusarium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008800:Mexico; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D003313:Zea mays / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D037341:Fumonisins; C056933:fumonisin B1