Gain-of-function screen for genes that affect Drosophila muscle pattern formation.
This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.
Staudt
Nicole
N
Max Planck Institut für biophysikalische Chemie, Göttingen, Germany.
Molitor
Andreas
A
Somogyi
Kalman
K
Mata
Juan
J
Curado
Silvia
S
Eulenberg
Karsten
K
Meise
Martin
M
Siegmund
Thomas
T
Häder
Thomas
T
Hilfiker
Andres
A
Brönner
Günter
G
Ephrussi
Anne
A
Rørth
Pernille
P
Cohen
Stephen M
SM
Fellert
Sonja
S
Chung
Ho-Ryun
HR
Piepenburg
Olaf
O
Schäfer
Ulrich
U
Jäckle
Herbert
H
Vorbrüggen
Gerd
G
D000818:Animals; D019521:Body Patterning; D002453:Cell Cycle; D002465:Cell Movement; D003599:Cytoskeleton / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D005821:Genetic Techniques; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D025801:Ubiquitin / Q000378:metabolism
D029721:Drosophila Proteins; D025801:Ubiquitin