title: LC/MS/MS measurement of penicillin G in bovine plasma, urine, and biopsy samples taken from kidneys of standing animals.
abstract: Methods for the measurement of penicillin concentration in bovine plasma, kidney and urine were developed and validated. Detection was based on liquid chromatography/tandem mass spectrometry (LC/MS/MS). Phenethecillin was used as an internal standard. Plasma was extracted with acetonitrile using a method with a calculated limit of quantitation (LOQ) of 12 ng/mL. Kidney samples were homogenized in water and acetonitrile, then cleaned up on C18-bonded silica SPE cartridges. The LOQ of this procedure was 10 ng/g. Urine samples were diluted, filtered, and analyzed directly. The LOQ of this procedure was 63 ng/mL. The overall accuracy for plasma was 103% with coefficient of variation (CV) of 3%; for kidney, 96% and 11%, respectively, and for urine, 98% and 4%, respectively. These methods were applied to the analysis of plasma, urine, and kidney biopsy samples taken from standing animals that had been dosed with penicillin.
authors.affiliation: Food and Drug Administration, Center for Veterinary Medicine, Laurel, MD, USA. david.heller@fda.gov
authors.forename: David N
authors.initials: DN
authors.lastname: Heller
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Smith
authors.forename: O Alberto
authors.initials: OA
authors.lastname: Chiesa
mesh_terms: D000818:Animals; D001706:Biopsy; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D007668:Kidney / Q000737:chemistry* / Q000473:pathology; D013058:Mass Spectrometry / Q000379:methods*; D010400:Penicillin G / Q000032:analysis* / Q000097:blood / Q000652:urine; D012015:Reference Standards; D012680:Sensitivity and Specificity
chemical_list: D010400:Penicillin G