title: Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat.
abstract: Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.
authors.affiliation: CPI Division, Rothamsted Research, Harpenden, AL5 2JQ, UK. huw.jones@bbsrc.ac.uk
authors.forename: Huw D
authors.initials: HD
authors.lastname: Jones
authors.forename: Angela
authors.initials: A
authors.lastname: Doherty
authors.forename: Huixia
authors.initials: H
authors.lastname: Wu