Modification of cytokinin levels in potato via expression of the Petunia hybrida Sho gene.
The Sho gene from Petunia hybrida encodes an enzyme for cytokinin synthesis. Here we report on the effects of Shogene expression on potato development. In contrast to transgenic potato expressing the Agrobacterium ipt gene, moderate Sho expression resulted in sufficient root development that allowed the cultivation of the Sho transformants in soil. The most pronounced effects detectable in these lines were an enhanced shoot production, delayed tuber formation, significant reduction in tuber size, and inhibition of tuber dormancy. Sho expression predominantly associated with a strong increase in 2iP glucosides, accompanied by an increase in zeatin glucosides in lines with very high Sho expression levels. The data demonstrate that it is possible to produce viable plants with enhanced cytokinin levels via constitutive Sho expression, which allows an assessment of cytokinin effects in all organs.
Zubko
Elena
E
Centre for Plant Sciences, The University of Leeds, LS2 9JT, UK.
Machácková
Ivana
I
Malbeck
Jiri
J
Meyer
Peter
P
D003583:Cytokinins / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D017343:Genes, Plant; D032306:Petunia / Q000201:enzymology / Q000235:genetics*; D010641:Phenotype; D010937:Plant Growth Regulators / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D014170:Transformation, Genetic
D003583:Cytokinins; D010937:Plant Growth Regulators; D018749:RNA, Plant