Analysis of yeast isolates related to Metschnikowia pulcherrima using the partial sequences of the large subunit rDNA and the actin gene; description of Metschnikowia andauensis sp. nov.
Thirty-two yeast isolates were cultured from guts or excrements of three different pests of corn or from the stem of healthy corn. The strains were analyzed using MSP-PCR (micro/minisatellite-primed polymerase chain reaction), sequences of the D1/D2 region of the large subunit rDNA and a 979 bp long part of the actin gene (act-1). They seem to belong to three groups that are all sister groups of Metschnikowia pulcherrima, M. fructicola and M. chrysoperlae. A new species, Metschnikowia andauensis (HA 1657T) is described. In contrast to M. pulcherrima and M. fructicola, M. andauensis is well separated in the act-1 phylogenetic tree too.
Molnár
Orsolya
O
Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Vienna, Austria.
Prillinger
Hansjörg
H
D000199:Actins / Q000235:genetics; D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D041981:Gastrointestinal Tract / Q000382:microbiology; D005800:Genes, Fungal; D007313:Insecta / Q000254:growth & development / Q000382:microbiology; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003313:Zea mays / Q000382:microbiology
D000199:Actins; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D005656:Fungal Proteins