The ABP-120 C-end region from Entamoeba histolytica interacts with sulfatide, a new lipid target.
EhABP-120 is the first filamin identified in the parasitic protozoan Entamoeba histolytica. Filamins are a family of cross-linking actin-binding proteins that promote a dynamic orthogonal web. They have been reported to interact directly with more than 30 cellular proteins and some phosphoinositides. The biochemical consequences of these interactions may have either positive or negative effects on the cross-linking function and also form a link between the cytoskeleton and plasma membrane. In this study, the EhABP-120 carboxy-terminal domain (END) was biochemically characterized. This domain was able to associate to 3-sulfate galactosyl ceramide, a new lipid target for a member of the filamin family. Also, the END domain was able to dimerize "in vitro." Molecular modeling analysis showed that the dimeric region is stabilized by a disulfide bond. Electrostatic and docking studies suggest that an electropositive concave pocket at the dimeric END domain interacts simultaneously with several sulfogalactose moieties of the sulfatide.
Díaz-Valencia
Juan Daniel
JD
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Mexico, DF, Mexico.
Almaraz-Barrera
Ma de Jesús
Mde J
Arias-Romero
Luis Enrique
LE
Hernandez-Rivas
Rosaura
R
Rojo-Domínguez
Arturo
A
Guillén
Nancy
N
Vargas
Miguel
M
D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003285:Contractile Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000235:genetics / Q000378:metabolism*; D064448:Filamins; D008840:Microfilament Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013379:Substrate Specificity; D013433:Sulfoglycosphingolipids / Q000378:metabolism*; D014162:Transfection
D003285:Contractile Proteins; D064448:Filamins; D008840:Microfilament Proteins; D013433:Sulfoglycosphingolipids