Computer-aided analysis of spatial structure of some hydrolytic enzymes.
Using the MolScript version 2.1 computer program for protein molecule modeling and X-ray structure analysis data the spatial structures of several hydrolytic enzymes have been compared. These include glucoamylase from Aspergillus awamori and Saccharomycopsis fibuligera and lipases from Rhizopus japonicus. Results on homology of amino acid sequences and topology of secondary structure elements were obtained. 3D models of these enzymes with positioning of functionally important groups in the active site cavity were built.
Artyukhov
V G
VG
Voronezh State University, Voronezh, 394006, Russia.
Kovaleva
T A
TA
Kozhokina
O M
OM
Bitutskaya
L A
LA
Dronov
R V
RV
Trofimova
O D
OD
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology*; D001665:Binding Sites; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012233:Rhizopus / Q000201:enzymology*; D012442:Saccharomycopsis / Q000201:enzymology*; D013379:Substrate Specificity
D008049:Lipase; D006026:Glycoside Hydrolases; D005087:Glucan 1,4-alpha-Glucosidase