Expression of bFGF and NGF and their receptors in chick's auditory organ following overexposure to noise.
Growth factors are known to activate signaling cascades for DNA replication; they participate in the regulation of cell differentiation and are required as positive signals for cell survival. Thus, many of them may be regarded as potential candidates stimulating regeneration processes in the inner ear. We analyzed the expression of basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) and their receptor (bFGFR and NGFR)-like immunoreactivity in chick basilar papillae, along with bFGF and NGF mRNA expression. The evaluation was made 1 and 5 days after exposure to wide-band noise with two increasing levels of acoustic energy. For both factors, the immunoreactivity was shown predominantly in the middle part of basilar papilla, in noise-exposed, but not control birds. It was localized in the cytoplasm of hair cells, nuclei of supporting cells and cytoplasm of ganglion cells. Strong immunoreactivity of bFGFR and NGFR was found both in control and noise-exposed animals, with the cell localization similar to that of growth factors. The increase in mRNA expression for bFGF and NGF was found in noise-exposed animals only after lower exposure to noise, on day 5 after exposure (p<0.01). A lack of increased expression after higher exposure could be excused by larger damage of hair cells followed by the increase of mRNA for beta-actin to which the results were referred. The results suggest bFGF and NGF involvement in postinjury regeneration of the basilar papilla.
Sliwinska-Kowalska
Mariola
M
Department of Physical Hazards, The Nofer Institute of Occupational Medicine, Teresy St. 8, 91-348 Lodz, Poland. marsliw@imp.lodz.pl
Rzadzinska
Agnieszka
A
Rajkowska
Elzbieta
E
Malczyk
Malgorzata
M
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002645:Chickens; D016222:Fibroblast Growth Factor 2 / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D006317:Hearing Loss, Noise-Induced / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D007150:Immunohistochemistry; D020932:Nerve Growth Factor / Q000235:genetics* / Q000378:metabolism*; D009925:Organ of Corti / Q000293:injuries / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000235:genetics* / Q000378:metabolism*; D020800:Receptor, Nerve Growth Factor / Q000235:genetics* / Q000378:metabolism*; D012038:Regeneration / Q000235:genetics / Q000502:physiology
D012333:RNA, Messenger; D020800:Receptor, Nerve Growth Factor; D016222:Fibroblast Growth Factor 2; D020932:Nerve Growth Factor; D051497:Receptor, Fibroblast Growth Factor, Type 2