Irreversible temperature-induced changes in purple membranes studied by electrooptics.
The thermal stability of purple membranes is studied by electric light scattering. Information on the polarization mechanisms is obtained. Incomplete recovery of the initial electric state (i.e., of permanent dipole moment, p( perpendicular), and electric polarizability, gamma(fast, ||)) after the membranes are heated at temperatures above 60 degrees C is revealed. Additional slow polarizability components, gamma(slow, perpendicular) and gamma(slow, ||), relaxing at different characteristic frequencies than the fast longitudinal polarizability gamma(fast, ||) appear in the temperature range where the order-disorder transition takes place. The slow polarizability gamma(slow, perpendicular) is probably connected with counterion displacement in the electrical double layer perpendicular to the disk plane. The results are important for understanding the polarization mechanisms and the origin of slow orienting moments.
Tuparev
N
N
Institute of Biophysics, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
Petkanchin
I B
IB
Taneva
S G
SG
D004563:Electrochemistry; D019617:Halobacterium salinarum / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D018717:Purple Membrane / Q000737:chemistry* / Q000378:metabolism*