title: Effect of bowel decontamination with metronidazole and vancomycin on gastroduodenal myoelectric activity.
abstract: It is well recognized that prolonged antibiotic therapy leading to gut decontamination often results in side effects and may lead to colonization of gut with pathologic bacteria. Changes of a gut microflora could play a role in dysmotility of gastrointestinal tract. The aim of the study was to evaluate influence of intraluminal colon anaerobic and aerobic bacterial flora on myoelectric activity of duodenum and stomach. A myoelectric activity recordings using electrodes implanted on small bowel of the conscious rats were performed. Group I was scheduled for control recording, group II for recordings in 4th day after metronidazole (M) administration (30 mg/kg) and group III for recordings after vancomycin (V) administration (15 mg/kg) respectively. Rat's stools were cultured for confirmation of changes in colon flora composition. Recordings were previously filtered digitally with bandwidth filter 0.01-0.1 Hz and 0.1-1.0 Hz to extract gastric and duodenal slow wave respectively and than analyzed with Fast Fourier Transformation. Baseline duodenal slow wave frequency in control group revealed 0.60 +/- 0.05 Hz. M increased slow waves frequency to 0.64 +/- 0.13 Hz and V did not 0.58 +/- 0.09 Hz (p > 0.05). Slow wave dominant frequency of the stomach showed decrease of frequency from control 0.035 +/- 0.04 to 0.025 +/- 0.06 Hz after M (p < 0.05). Pretreatment with V also did not influence slow wave dominant frequency in comparison to control group (0.036 +/- 0.07 Hz, p > 0.05).
  
  
  CONCLUSIONS
  Only pretreatment with M significantly decreased gastric slow wave frequency. One can speculate that M effects are related not only to gut decontamination but also directly affects ENS. We propose hypothesis that M influence on slow wave frequency may be related not only to its antimicrobial activity but to its potential neurotoxic action on intramural ENS neurons.
authors.affiliation: Katedra Patofizjologii, Collegium Medicum Uniwersytetu Jagiellońskiego.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Królczyk
authors.forename: Antoni
authors.initials: A
authors.lastname: Czupryna
authors.forename: Jacek
authors.initials: J
authors.lastname: Sobocki
authors.forename: Lukasz
authors.initials: L
authors.lastname: Nowak
authors.forename: Daniel
authors.initials: D
authors.lastname: Zurowski
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Szatyłowiczi
authors.forename: Magdalena
authors.initials: M
authors.lastname: Strus
authors.forename: Piotr J
authors.initials: PJ
authors.lastname: Thor
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D004386:Duodenum / Q000382:microbiology / Q000503:physiopathology; D005260:Female; D005769:Gastrointestinal Motility / Q000187:drug effects*; D008795:Metronidazole / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000382:microbiology / Q000503:physiopathology; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D008795:Metronidazole; D014640:Vancomycin