Analysis of cytochrome P450 genes in silkworm genome (Bombyx mori).
We have searched the Bombyx mori genome for members of the major enzyme family, the Cytochrome P450s, which carry out multiple reactions to enable organisms to rid themselves of foreign compounds. As a result, 86 putative P450s were discovered in silkworm genome, which are thought to belong to 32 subfamilies. A comparative genomic analysis with Drosophila melanogaster reveals that the two insects have some similar P450 distribution patterns but still have some obvious differences. Especially, the diverse distribution exists in 7 p450 subfamilies, which are CYP4A, CYP4C, CYP4D, CYP6A, CYP6AE, CYP6B and CYP9A. Furthermore, we collected expression sequence tag (EST) evidence for 49 putative P450s genes, which are expressed at the transcriptional level and more likely to be true P450s.
Li
Bin
B
Key Sericultural Laboratory of Agricultural Ministry, College of Sericulture & Biotechnology, Southwest Agricultural University, Chongqing, China.
Xia
Qingyou
Q
Lu
Cheng
C
Zhou
Zeyang
Z
Xiang
Zhonghuai
Z
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000201:enzymology* / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics*; D049750:Genome, Insect; D006801:Humans; D005810:Multigene Family; D010944:Plants / Q000235:genetics / Q000469:parasitology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D003577:Cytochrome P-450 Enzyme System