title: Cell context reveals a dual role for Maf in oncogenesis.
abstract: Maf b-Zip transcription factors are involved in both terminal differentiation and oncogenesis. To investigate this apparent contradiction, we used two different primary cell types and performed an extensive analysis of transformation parameters induced by Maf proteins. We show that MafA and c-Maf are potent oncogenes in chicken embryo fibroblasts, while MafB appears weaker. We also provide the first evidence that MafA can confer growth factor independence and promote cell division at low density. Moreover, using MafA as a model, we identified several parameters that are critical for Maf transforming activities. Indeed, MafA ability to induce anchorage-independent cell growth was sensitive to culture conditions. In addition, the transforming activity of MafA was dependent on its phosphorylation state, since mutation on Ser65 impaired its ability to induce growth at low density and anchorage-independent growth. We next examined transforming activity of large Maf proteins in embryonic neuroretina cells, where they are known to induce differentiation. Unlike v-Jun, MafA, MafB and c-Maf did not show oncogenic activity in these cells. Moreover, they counteracted transformation induced by constitutive activation of the Ras/Raf/MEK pathway. Taken together, our results show that Maf proteins could display antagonistic functions in oncogenesis depending on the cellular context, and support a dual role for Maf as both oncogenes and tumor suppressor-like proteins.
authors.affiliation: Institut Curie, CNRS UMR 146, Centre Universitaire, Laboratoire 110, Orsay Cedex, France. Celio.Pouponnot@curie.u-psud.fr
authors.forename: C
authors.initials: C
authors.lastname: Pouponnot
authors.forename: K
authors.initials: K
authors.lastname: Sii-Felice
authors.forename: I
authors.initials: I
authors.lastname: Hmitou
authors.forename: N
authors.initials: N
authors.lastname: Rocques
authors.forename: L
authors.initials: L
authors.lastname: Lecoin
authors.forename: S
authors.initials: S
authors.lastname: Druillennec
authors.forename: M-P
authors.initials: MP
authors.lastname: Felder-Schmittbuhl
authors.forename: A
authors.initials: A
authors.lastname: Eychène
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002455:Cell Division; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002642:Chick Embryo / Q000166:cytology; D005347:Fibroblasts; D016147:Genes, Tumor Suppressor; D006801:Humans; D051473:Maf Transcription Factors, Large / Q000502:physiology*; D009857:Oncogenes; D010766:Phosphorylation; D010957:Plasmids; D051257:Proto-Oncogene Proteins c-maf / Q000502:physiology*; D012160:Retina / Q000166:cytology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C495607:MAF protein, human; C485159:MAFA protein, human; D051473:Maf Transcription Factors, Large; D051257:Proto-Oncogene Proteins c-maf