title: The fission yeast gene encoding monothiol glutaredoxin 5 is regulated by nitrosative and osmotic stresses.
abstract: Glutaredoxin (Grx) is a small, heat-stable redox protein acting as a multi-functional glutathione (GSH)-dependent disulfide oxidoreductase. We have cloned the monothiol Grx5 gene from the genomic DNA of the fission yeast Schizosaccharomyces pombe. It has 1,904 bp, with one intron, and encodes a putative protein of 146 amino acids with a molecular mass of 16.5 kDa. Recombinant Grx5 produced functional Grx in S. pombe cells. NO-generating sodium nitroprusside (SNP, 1.0 and 2.0 mM) and potassium chloride (KCl, 0.2 and 0.5 M) increased the synthesis of beta-galactosidase from a Grx5-lacZ fusion gene, and transcription of Grx5 was also enhanced by SNP and KCl. Synthesis of beta-galactosidase from the Grx5-lacZ fusion was lower in Pap1-negative TP108-3C cells than in wild type KP1 cells, and when Pap1 was overproduced in KP1 cells, the level of beta-galactosidase increased. We also found that Pap1 is involved in the induction of Grx5 by SNP and KCl. S. pombe Grx5 may play a crucial role in responses to nitrosative and osmotic stresses.
authors.affiliation: Division of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Hong-Gyum
authors.initials: HG
authors.lastname: Kim
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Park
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Lim
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D054477:Glutaredoxins; D008969:Molecular Sequence Data; D009599:Nitroprusside / Q000378:metabolism* / Q000494:pharmacology; D009997:Osmotic Pressure; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D000073718:Pancreatitis-Associated Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000494:pharmacology; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism / Q000494:pharmacology; D016415:Sequence Alignment; D013997:Time Factors; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D054477:Glutaredoxins; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; C000612141:REG3A protein, human; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D029702:Schizosaccharomyces pombe Proteins; D009599:Nitroprusside; D010088:Oxidoreductases; D001616:beta-Galactosidase