title: Molecular cloning and expression of putrescine N-methyltransferase from the hairy roots of Anisodus tanguticus.
abstract: A full-length cDNA encoding putrescine N-methyltransferase (PMT) was isolated from the hairy roots of A. tanguticus. Nucleotide sequence analysis of the cloned cDNA revealed an open reading frame of 1017 bp encoding 338 amino acids with high homology to other known PMTs. A. tanguticus PMT was expressed in Escherichia coli. The recombinant AtPMT was purified and exhibited S-adenosyl-methionine-dependent N-methyltransferase activity.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, #1 Xian Nong Tan Street, Beijing 100050, P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Ke-di
authors.initials: KD
authors.lastname: Cheng
authors.forename: Chao
authors.initials: C
authors.lastname: Meng
authors.forename: Hui-xin
authors.initials: HX
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D008517:Phytotherapy; D018517:Plant Roots; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D019657:Solanaceae / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D008780:Methyltransferases; C112598:putrescine N-methyltransferase