title: [Determination of two main components in bark of Paeonia suffruticosa by HPLC].
abstract: OBJECTIVE
  To establish a HPLC method for determination two constituents in bark of Paeonia Suffuticosa.
  
  
  METHODS
  The reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm x 150 mm, 5 microm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL x min(-1) and UV detection was set at 274 nm.
  
  
  RESULTS
  The assay displayed good linearity over the concentration ranges of 0.06-1.0 microg (r = 0.999 9, gallic acid) and 0.16-2.58 microg (r = 0.999 9, paeonol) respectively. The average recoveries (n = 9) of gallic acid and paeonol were 98.6% (RSD = 3.0%), 98.2% (RSD = 2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration.
  
  
  CONCLUSIONS
  The method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Befiing 100050, China.
authors.forename: Yuan-zi
authors.initials: YZ
authors.lastname: Tian
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jian-zhen
authors.initials: JZ
authors.lastname: Liu
authors.forename: Bei-bei
authors.initials: BB
authors.lastname: Yang
authors.forename: Jian-gong
authors.initials: JG
authors.lastname: Shi
authors.forename: Mu-zou
authors.initials: MZ
authors.lastname: Wang
mesh_terms: D000098:Acetophenones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005707:Gallic Acid / Q000032:analysis*; D029596:Paeonia / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
chemical_list: D000098:Acetophenones; C013638:paeonol; D005707:Gallic Acid