title: Analysis of allele fidelity, polymorphic information content, and density of microsatellites in a genome-wide screening for hip dysplasia in a crossbreed pedigree.
abstract: Recent advances in genomics resources and tools are facilitating quantitative trait locus mapping. We developed a crossbreed pedigree for mapping quantitative trait loci for hip dysplasia in dogs by crossing dysplastic Labrador Retrievers and normal Greyhounds. We show that one advantage to using a crossbreed pedigree is the increased marker informativeness in the backcross/F2 population relative to the founder populations. We also discuss three factors that affect the detection power in the context of this crossbreed pedigree: being able to detect and correct genotyping errors, increasing marker density for chromosomes with a sparse coverage, and adding individuals to the mapping population as soon as they become available.
authors.affiliation: Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, NY 14853, USA.
authors.forename: R G
authors.initials: RG
authors.lastname: Mateescu
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: K
authors.initials: K
authors.lastname: Tsai
authors.forename: J
authors.initials: J
authors.lastname: Phavaphutanon
authors.forename: N I
authors.initials: NI
authors.lastname: Burton-Wurster
authors.forename: G
authors.initials: G
authors.lastname: Lust
authors.forename: R
authors.initials: R
authors.lastname: Quaas
authors.forename: K
authors.initials: K
authors.lastname: Murphy
authors.forename: G M
authors.initials: GM
authors.lastname: Acland
authors.forename: R J
authors.initials: RJ
authors.lastname: Todhunter
mesh_terms: D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D004285:Dogs; D005260:Female; D006619:Hip Dysplasia, Canine / Q000235:genetics*; D006824:Hybridization, Genetic; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010375:Pedigree; D011110:Polymorphism, Genetic / Q000235:genetics*; D015203:Reproducibility of Results; D012107:Research Design; D013223:Statistics as Topic / Q000379:methods