Identification by differential display of IL-24 autocrine loop activated by ras oncogenes.
Ras signaling pathway is thought to control the expression of a subset of yet-to-be-defined genes that are crucial for cell growth and differentiation. Here we have identified by differential display a novel oncogenic Ras target gene encoding a new cytokine. Biochemical studies reveal that this cytokine, which we named IL-24, is a member of IL-10 family of cytokines, and it signals through two hetorodimeric receptors, whose expression is also upregulated by ras oncogenes. Thus, IL-24 and its receptors may represent a novel autocrine loop coordinately activated by ras oncogenes.
Tan
Zhongjia
Z
Department of Cell Biology, Vanderbilt-Ingram Cancer Center, School of Medicine, Vanderbilt University, Nashville, TN, USA.
Wang
Mai
M
Liang
Peng
P
D015153:Blotting, Western; D002454:Cell Differentiation; D016207:Cytokines / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000737:chemistry; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D007378:Interleukins / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D018631:ras Proteins / Q000235:genetics*; D000099180:Interleukin-24
D016207:Cytokines; D018076:DNA, Complementary; D003850:Deoxyribonuclease I; D016753:Interleukin-10; D007378:Interleukins; D012313:RNA; D012333:RNA, Messenger; D018631:ras Proteins; D000099180:Interleukin-24