title: Miniaturized glucose biosensor modified with a nitric oxide-releasing xerogel microarray.
abstract: An enzyme-based glucose biosensor modified to release nitric oxide (NO) via a xerogel microarray is reported. The biosensor design is as follows: (1) glucose oxidase (GOx) is immobilized in a methyltrimethoxysilane (MTMOS) xerogel layer; (2) a blended polyurethane/hydrophilic polyurethane coating prevents enzyme leaching and imparts selectivity for glucose; and (3) micropatterned xerogel lines (5 microm wide) separated by distances of 5 or 20 microm provide NO-release capability. This configuration allows for increased glucose sensitivity relative to sensors modified with NO-releasing xerogel films since significant portions of the sensor surface remain unmodified. Glucose diffusion to the GOx layer is thus less inhibited. The micropatterned NO-releasing biosensors generate sufficient NO levels to reduce both Pseudomonas aeruginosa and platelet adhesion without significantly compromising the enzymatic activity of GOx. The glucose response, linearity and stability of the NO-releasing micropatterned sensors are reported.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA.
authors.forename: Bong Kyun
authors.initials: BK
authors.lastname: Oh
authors.forename: Mary E
authors.initials: ME
authors.lastname: Robbins
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Nablo
authors.forename: Mark H
authors.initials: MH
authors.lastname: Schoenfisch
mesh_terms: D000042:Absorption; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation / Q000379:methods; D001792:Blood Platelets / Q000187:drug effects; D002478:Cells, Cultured; D004058:Diffusion; D016503:Drug Delivery Systems / Q000295:instrumentation* / Q000379:methods; D004866:Equipment Contamination / Q000517:prevention & control*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005782:Gels / Q000737:chemistry; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization; D009569:Nitric Oxide / Q000008:administration & dosage* / Q000737:chemistry; D015539:Platelet Activation / Q000187:drug effects; D011552:Pseudomonas Infections / Q000517:prevention & control; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000187:drug effects*; D013552:Swine
chemical_list: D001786:Blood Glucose; D005782:Gels; D009569:Nitric Oxide