title: Integration of digital signal processing technologies with pulsed electron paramagnetic resonance imaging.
abstract: The integration of modern data acquisition and digital signal processing (DSP) technologies with Fourier transform electron paramagnetic resonance (FT-EPR) imaging at radiofrequencies (RF) is described. The FT-EPR system operates at a Larmor frequency (L(f)) of 300MHz to facilitate in vivo studies. This relatively low frequency L(f), in conjunction with our approximately 10MHz signal bandwidth, enables the use of direct free induction decay time-locked subsampling (TLSS). This particular technique provides advantages by eliminating the traditional analog intermediate frequency downconversion stage along with the corresponding noise sources. TLSS also results in manageable sample rates that facilitate the design of DSP-based data acquisition and image processing platforms. More specifically, we utilize a high-speed field programmable gate array (FPGA) and a DSP processor to perform advanced real-time signal and image processing. The migration to a DSP-based configuration offers the benefits of improved EPR system performance, as well as increased adaptability to various EPR system configurations (i.e., software configurable systems instead of hardware reconfigurations). The required modifications to the FT-EPR system design are described, with focus on the addition of DSP technologies including the application-specific hardware, software, and firmware developed for the FPGA and DSP processor. The first results of using real-time DSP technologies in conjunction with direct detection bandpass sampling to implement EPR imaging at RF frequencies are presented.
authors.affiliation: Signal Processing and Instrumentation Section, Division of Computational Biosciences, Center for Information Technology, National Institutes of Health, 12 South Drive, Bldg. 12A-2025, Bethesda, MD 20892-1002, USA. pursley@nih.gov
authors.forename: Randall H
authors.initials: RH
authors.lastname: Pursley
authors.forename: Ghadi
authors.initials: G
authors.lastname: Salem
authors.forename: Nallathamby
authors.initials: N
authors.lastname: Devasahayam
authors.forename: Sankaran
authors.initials: S
authors.lastname: Subramanian
authors.forename: Janusz
authors.initials: J
authors.lastname: Koscielniak
authors.forename: Murali C
authors.initials: MC
authors.lastname: Krishna
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Pohida
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000295:instrumentation*; D004867:Equipment Design; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D012984:Software; D013113:Spin Labels
chemical_list: D013113:Spin Labels