Chemical characteristics and cytocompatibility of collagen-based scaffold reinforced by chitin fibers for bone tissue engineering.
Chitin is a kind of seemly material to match PLLA for a scaffold, which may create an appropriate environment for the regeneration of tissues. In this study, we prepared and evaluated a new nano-hydroxyapatite/collagen/PLLA (nHACP) scaffold reinforced by chitin fibers for bone-tissue engineering. The chitin fibers were crosslinked with PLLA by dicyclohexylcarbodimide (DCC). The chemical characteristics were evaluated by Fourier transformed infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The mechanical strength was measured by compressive tests. The fibers, crosslinked with PLLA, could enhance the compressive strength of the scaffold about four times. Human marrow mesenchymal stem cells (MSCs) culture showed that the reinforced nHACP scaffolds were more cytocompatible than that without reinforcement. The crosslinks hardly affected the cytocompatibility of the reinforced scaffolds. The results suggested that the reinforced scaffolds (DCC crosslinked) might be a promising candidate for bone-tissue engineering.
Li
Xiaoming
X
Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.
Feng
Qingling
Q
Wang
Wenjie
W
Cui
Fuzhai
F
D000818:Animals; D001842:Bone and Bones; D002417:Cattle; D002686:Chitin / Q000627:therapeutic use*; D024042:Collagen Type I / Q000627:therapeutic use*; D019245:Compressive Strength; D006801:Humans; D019344:Lactic Acid; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology; D011091:Polyesters; D011108:Polymers; D023822:Tissue Engineering / Q000379:methods* / Q000592:standards
D024042:Collagen Type I; D011091:Polyesters; D011108:Polymers; D002686:Chitin; D019344:Lactic Acid; C033616:poly(lactide)