Multithermal titration calorimetry: a rapid method to determine binding heat capacities.
Herein a new method that allows binding DeltaCp to be determined with a single experiment is presented. Multithermal titration calorimetry (MTC) is a simple extension of isothermal titration calorimetry (ITC) that explicitly takes into account the thermal dependences of DeltaH and the binding constant. Experimentally, this is accomplished by performing a single stepwise titration with ITC equipment, allowing temperature re-adjustments of the system at intermediate states of the titration process. Thus, from the resulting multitherm, DeltaCp can also be determined. The experimental feasibility of MTC was tested by using the well-characterized lysozyme-chitotriose complex as a model system.
Chavelas
Eneas A
EA
Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior, Cd. Universitaria, México D.F., México 04510.
Zubillaga
Rafael A
RA
Pulido
Nancy O
NO
García-Hernández
Enrique
E
D000465:Algorithms; D002151:Calorimetry / Q000379:methods*; D005240:Feasibility Studies; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism*; D008956:Models, Chemical; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors
D046911:Macromolecular Substances