Hyperglycemia increases superoxide production in the CA1 pyramidal neurons after global cerebral ischemia.
Transient global cerebral ischemia results in selective neuronal death in the vulnerable hippocampal CA1 pyramidal neurons in a delayed manner. Hyperglycemia accelerates and exacerbates neuronal damage in this region. The object of this study was to determine whether hyperglycemia-enhanced damage is associated with increased production of superoxide anion after ischemia. The results showed that hyperglycemic ischemia caused a significant increase of superoxide production in the hippocampal CA1 neurons compared to normoglycemic animals after 18 h of recirculation, suggesting that enhanced superoxide anion production may mediate the hyperglycemia-accelerated and -enhanced neuronal death in the hippocampal CA1 area after ischemia and reperfusion.
Muranyi
Marianna
M
Department of Cell, John A. Burns School of Medicine, University of Hawaii at Manoa, 1960 East-West Road, Biomedical Tower T514, Honolulu, HI 96822, USA.
Li
Ping-An
PA
D000704:Analysis of Variance; D000818:Animals; D002545:Brain Ischemia / Q000201:enzymology* / Q000473:pathology; D004195:Disease Models, Animal; D004996:Ethidium / Q000031:analogs & derivatives / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000473:pathology*; D006943:Hyperglycemia / Q000201:enzymology* / Q000473:pathology; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D017966:Pyramidal Cells / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism*
D005904:Glial Fibrillary Acidic Protein; C067883:dihydroethidium; D013481:Superoxides; D010751:Phosphopyruvate Hydratase; D004996:Ethidium