Evaluation of dipicolinic acid for detection of IMP- or VIM- type metallo-beta-lactamase-producing Pseudomonas aeruginosa clinical isolates.
We evaluated dipicolinic acid (DPA) as a chelating agent for detection of IMP- or VIM-type metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa clinical isolates. Using the broth microdilution testing in the presence or absence of DPA, MBL producers exhibited 100%, 92%, or 100% of >or=8 times (media, 32 times) reduction of MICs in presence of DPA for ceftazidime, imipenem, or meropenem, respectively. In disk diffusion testing, expansion of growth inhibitory zone of these clinical isolates was clearly observed. Thus, DPA could be useful in the detection for MBL-producing P. aeruginosa clinical isolates.
Kimura
Soichiro
S
Department of Microbiology and Infectious Disease, Toho University School of Medicine, Tokyo 1438540, Japan.
Ishii
Yoshikazu
Y
Yamaguchi
Keizo
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002442:Ceftazidime / Q000494:pharmacology; D002614:Chelating Agents / Q000737:chemistry*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D000077731:Meropenem; D008826:Microbial Sensitivity Tests / Q000379:methods; D010848:Picolinic Acids / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification; D013845:Thienamycins / Q000494:pharmacology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D002614:Chelating Agents; D010848:Picolinic Acids; D013845:Thienamycins; D015378:Imipenem; D002442:Ceftazidime; C409201:beta-lactamase IMP-1; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases; D000077731:Meropenem; C004860:dipicolinic acid