[Experimental study on induction of the normal human melanocytes apoptosis in vitro by cycloheximide and TNF-alpha].
OBJECTIVE
To investigate the effects of cycloheximide and TNF-alpha on melanocyte (MC).


METHODS
Melanocyte apoptosis was studied with MTT, transmitting electron microscopy and fluorescence labeling of alive cells.


RESULTS
We added TNF-alpha and cycloheximide in melanocytes, and the typical apoptosis appeared 24 hours later, with chromatin condensation, nuclear pyknosis and apoptotic bodies formation. The results of cytometry showed the typical apoptotic peak.


CONCLUSIONS
TNF-alpha and cycloheximide together could inhibit MC proliferation and induce MC apoptosis.
Yang
Zhuang-Qun
ZQ
Department of Oral and Maxillofacial Plastic Surgery, Stomatological College, Xi'an Jiao Tong University, Xi'an 710004, China.
Li
Peng
P
Tu
Jun-Bo
JB
Wang
Zheng-Hui
ZH
Hu
Xiao-Yi
XY
Zhang
Tie-Liang
TL
D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation; D003513:Cycloheximide / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008544:Melanocytes / Q000166:cytology* / Q000187:drug effects*; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D055815:Young Adult
D014409:Tumor Necrosis Factor-alpha; D003513:Cycloheximide