title: Fluoride dose response in pH-cycling models using bovine enamel.
abstract: The aim of this study was to establish methodologies for verification of the fluoride solution dose-response relationship using bovine enamel and pH-cycling models. Six models of the cariogenic challenge were performed, varying the time of demineralization and pH, time of remineralization, composition of de- and remineralization solutions, frequency and time of application of treatment solutions and pH-cycling duration. For the evaluation of the fluoride effect on caries dynamics, two proposed models provided for improvement in standardization of methods leading to a higher level of precision, demonstrating a dose response between treatments with regard to surface microhardness and DeltaZ. For the evaluation of the fluoride effect on enamel remineralization, the addition of fluoride to the de- and remineralization solutions and the reduction of frequency and time of application of fluoride solutions led to a more suitable pH-cycling model.
authors.affiliation: UNESP, São Paulo State University, School of Dentistry at Araçatuba, São Paulo, Brazil.
authors.forename: Ana Elisa de Mello
authors.initials: AE
authors.lastname: Vieira
authors.forename: Alberto Carlos Botazzo
authors.initials: AC
authors.lastname: Delbem
authors.forename: Kikue Takebayashi
authors.initials: KT
authors.lastname: Sassaki
authors.forename: Eliana
authors.initials: E
authors.lastname: Rodrigues
authors.forename: Jaime Aparecido
authors.initials: JA
authors.lastname: Cury
authors.forename: Robson Frederico
authors.initials: RF
authors.lastname: Cunha
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002327:Cariostatic Agents / Q000008:administration & dosage*; D002417:Cattle; D003731:Dental Caries / Q000188:drug therapy; D003743:Dental Enamel / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005459:Fluorides / Q000008:administration & dosage*; D006244:Hardness / Q000187:drug effects; D018709:Statistics, Nonparametric; D014089:Tooth Remineralization
chemical_list: D002327:Cariostatic Agents; D005459:Fluorides