title: Corn silk induced cyclooxygenase-2 in murine macrophages.
abstract: Stimulation of murine macrophages with corn silk induced cyclooxygenase (COX)-2 with secretion of PGE2. Expression of COX-2 was inhibited by pyrolidine dithiocarbamate (PDTC), and increased DNA binding by nuclear factor kappa B (NF-kappaB), indicating that COX-2 induction proceeds also via the NF-kappaB signaling pathway. A specific inhibitor of COX-2 decreased the expression level of inducible nitric oxide synthase (iNOS) stimulated by corn silk. PGE2 elevated the expression level of iNOS, probably via EP2 and EP4 receptors on the surface of the macrophages.
authors.affiliation: Department of Biological Sciences and the Immunomodulation Research Center, University of Ulsan, Korea.
authors.forename: Kyung A
authors.initials: KA
authors.lastname: Kim
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Shin
authors.forename: Sang Kyu
authors.initials: SK
authors.lastname: Choi
authors.forename: Hye-Seon
authors.initials: HS
authors.lastname: Choi
mesh_terms: D000818:Animals; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000235:genetics*; D015232:Dinoprostone / Q000378:metabolism / Q000502:physiology; D025461:Feedback, Physiological; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D018514:Plant Structures; D010946:Plants, Medicinal; D018078:Receptors, Prostaglandin E / Q000502:physiology; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D003313:Zea mays
chemical_list: D016328:NF-kappa B; C545645:Ptger2 protein, mouse; C545651:Ptger4 protein, mouse; D018078:Receptors, Prostaglandin E; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D015232:Dinoprostone