title: Identification of the major metabolites of resveratrol in rat urine by HPLC-MS/MS.
abstract: To identify the major metabolites of resveratrol in rat, rat urine samples were pretreated by using solid-phase extraction technique (SPE) with polyamide cartridges. And a LC-MS/MS method with electrospray ionisation (ESI), negative ion mode and collision induced dissociation (CID), was used to elucidate the structures of the major metabolites of resveratrol. According to the results of our experiment, we found that the main metabolites of resveratrol were resveratrol monoglucuronide (M1), dihydroresveratrol monosulfate (M2), resveratrol monosulfate (M3) and dihydroresveratrol (M4).
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, NanJing 210009, China. wycpu@tom.com
authors.forename: Donggeng
authors.initials: D
authors.lastname: Wang
authors.forename: Taijun
authors.initials: T
authors.lastname: Hang
authors.forename: Chunyong
authors.initials: C
authors.lastname: Wu
authors.forename: Wenying
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013267:Stilbenes / Q000493:pharmacokinetics / Q000652:urine*
chemical_list: D013267:Stilbenes; D000077185:Resveratrol