Identification of the major metabolites of resveratrol in rat urine by HPLC-MS/MS.
To identify the major metabolites of resveratrol in rat, rat urine samples were pretreated by using solid-phase extraction technique (SPE) with polyamide cartridges. And a LC-MS/MS method with electrospray ionisation (ESI), negative ion mode and collision induced dissociation (CID), was used to elucidate the structures of the major metabolites of resveratrol. According to the results of our experiment, we found that the main metabolites of resveratrol were resveratrol monoglucuronide (M1), dihydroresveratrol monosulfate (M2), resveratrol monosulfate (M3) and dihydroresveratrol (M4).
Wang
Donggeng
D
Department of Pharmaceutical Analysis, China Pharmaceutical University, NanJing 210009, China. wycpu@tom.com
Hang
Taijun
T
Wu
Chunyong
C
Liu
Wenying
W
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013267:Stilbenes / Q000493:pharmacokinetics / Q000652:urine*
D013267:Stilbenes; D000077185:Resveratrol