title: Integration of topographical and biochemical cues by axons during growth on microfabricated 3-D substrates.
abstract: During embryonic neural development, axon tips ("growth cones") are guided through a dynamic three-dimensional (3-D) landscape by soluble chemotropic factors and by immobilized, growth-permissive or growth-inhibiting contact cues present in the extracellular matrix and on the surface of surrounding cells. It has been difficult to probe the search algorithms of growth cones in response to multiple contact cues during 3-D navigation using traditional two-dimensional (2-D) substrates. Here, we present an in vitro study in which the axons of murine embryonic cortical neurons are challenged with competing growth options, using 3-D substrates that feature variations in permissiveness and microtopography. As 3-D substrates, we used poly-D-lysine (PDL) coatings on microfabricated steps of polydimethylsiloxane (PDMS) and complementary features of Matrigel. We found that axons display a preference for PDL over Matrigel and for the straightest path within a distance consistent with the exploratory range of the growth cone. When these two preferences are in conflict, axons choose to grow straight into Matrigel; when the straight path is not permissive, the axon turns in the direction that minimizes the turning angle. These results suggest that growth cones make 3-D navigation decisions by integrating permissiveness and topographical cues.
authors.affiliation: Department of Bioengineering, Campus Box 352255, University of Washington, Seattle, WA 98195-2255, USA.
authors.forename: Nianzhen
authors.initials: N
authors.lastname: Li
authors.forename: Albert
authors.initials: A
authors.lastname: Folch
mesh_terms: D000818:Animals; D001369:Axons / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D018929:Cell Culture Techniques; D002540:Cerebral Cortex / Q000166:cytology; D003094:Collagen / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004338:Drug Combinations; D020439:Growth Cones / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D007797:Laminin / Q000737:chemistry; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000502:physiology; D011107:Polylysine / Q000494:pharmacology; D011509:Proteoglycans / Q000737:chemistry; D012828:Silicones / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D012828:Silicones; C059485:matrigel; D011107:Polylysine; C013830:baysilon; D003094:Collagen