[Expression of feedback-resistant aspartate kinase gene in Corynebacterium crenatum].
The AEC-resistant aspartate kinase gene from C. crenatum CD945 was cloned into vector pJC1. Its expression was investigated both in the wild type C. crenatum AS1.542 and its mutant C. crenatum CD945. The result showed that C. crenatum AS1.542 harboring AK(fbr) gene could grow on the defined medium with the co-existence of 12 mg/mL both of AEC and L-threonine respectively. Overexpression of AK(fbr) gene in C. crenatum CD945 results in a 4-fold increase of specific enzyme activity than the parental strain. The amplification of the activity of aspartate kinase yields 22% increase of L-lysine production and 23% increase of L-lysine productivity without affecting the growth rate.
Zhao
Zhi
Z
Microbial Biotechnology Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Liu
Yang-Jian
YJ
Wang
Yu
Y
Zhang
Ying-Zi
YZ
Ding
Jiu-Yuan
JY
D001222:Aspartate Kinase / Q000235:genetics*; D003352:Corynebacterium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D025461:Feedback, Physiological; D005285:Fermentation; D008239:Lysine / Q000096:biosynthesis; D011995:Recombination, Genetic
D001222:Aspartate Kinase; D008239:Lysine