title: Association between human leukocyte antigen class II alleles and genotype of Borrelia burgdorferi in patients with early lyme disease.
abstract: BACKGROUND
  On the basis of a polymerase chain reaction-restriction fragment-length polymorphism analysis of the 16S-23S ribosomal DNA intergenic spacer, clinical isolates of Borrelia burgdorferi can be classified into 3 genotypes designated as RST1, RST2, and RST3. RST1 strains are the most pathogenic, and RST3 strains are the least pathogenic.
  
  
  METHODS
  Human leukocyte antigen (HLA) class II alleles were determined for a group of culture-positive patients with Lyme disease-associated erythema migrans and were evaluated for an association with the genotype of the infecting B. burgdorferi strain.
  
  
  RESULTS
  The DRB1*0101 allele carriage rate was higher in patients infected with RST3 strains (9/25 [36.0%]) than in patients infected with RST1 strains (2/28 [7.1%]) or RST2 strains (7/36 [19.4%]) (P=.010). The same relationship was found for carriage of the DRB1*0101-DQB1*0501 haplotype (P=.018), because of tight linkage disequilibrium. Similar associations could not be demonstrated for any of the other DRB1 and DQB1 alleles or haplotypes that were assessed.
  
  
  CONCLUSIONS
  The DRB1*0101 allele and the DRB1*0101-DQB1*0501 haplotype may be relevant to the development of infection with strains from the least invasive genotypes of B. burgdorferi.
authors.affiliation: Department of Medicine, Division of Infectious Diseases, New York Medical College, Valhalla, NY 10595, USA. gary_wormser@nymc.edu
authors.forename: Gary P
authors.initials: GP
authors.lastname: Wormser
authors.forename: Richard
authors.initials: R
authors.lastname: Kaslow
authors.forename: Jianming
authors.initials: J
authors.lastname: Tang
authors.forename: Karen
authors.initials: K
authors.lastname: Wade
authors.forename: Dionysios
authors.initials: D
authors.lastname: Liveris
authors.forename: Ira
authors.initials: I
authors.lastname: Schwartz
authors.forename: Mark
authors.initials: M
authors.lastname: Klempner
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D025065:Borrelia burgdorferi / Q000145:classification* / Q000235:genetics*; D015787:Erythema Chronicum Migrans / Q000235:genetics* / Q000382:microbiology; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D008193:Lyme Disease / Q000235:genetics* / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D000949:Histocompatibility Antigens Class II