Specific detection of RNA on ultra-thin sections.
In this paper, we describe a specific method for ultrastructural detection of RNA. Our method is based in the bromination "in situ" of uridine residues in the RNA, which allows the detection of brominated RNA with specific antibodies against bromo-deoxyuridine. With this method we can achieve high specificity and resolution, and it can be applied to Epon or acrylic resin embedded material.
Calabuig
Juana
J
INSTRACAN, Institute of Cancer Research, Juan Chaumel 16, Villena 03400, Alicante, Spain.
de Juan
Joaquin
J
Iborra
Francisco J
FJ
D000906:Antibodies / Q000378:metabolism; D001966:Bromine / Q000737:chemistry; D005453:Fluorescence; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008854:Microscopy, Electron; D008867:Microtomy; D012334:RNA, Neoplasm / Q000378:metabolism / Q000648:ultrastructure*; D012680:Sensitivity and Specificity; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation; D014529:Uridine / Q000276:immunology / Q000378:metabolism; D014867:Water / Q000737:chemistry
D000906:Antibodies; D012334:RNA, Neoplasm; D014867:Water; D001966:Bromine; D014529:Uridine