title: Fabrication and endothelialization of collagen-blended biodegradable polymer nanofibers: potential vascular graft for blood vessel tissue engineering.
abstract: Electrospun collagen-blended poly(L-lactic acid)-co-poly(epsilon-caprolactone) [P(LLA-CL), 70:30] nanofiber may have great potential application in tissue engineering because it mimicks the extracellular matrix (ECM) both morphologically and chemically. Blended nanofibers with various weight ratios of polymer to collagen were fabricated by electrospinning. The appearance of the blended nanofibers was investigated by scanning electron microscopy and transmission electron microscopy. The nanofibers exhibited a smooth surface and a narrow diameter distribution, with 60% of the nanofibers having diameters between 100 and 200 nm. Attenuated total reflectance-Fourier transform infrared spectra and X-ray photoelectron spectroscopy verified the existence of collagen molecules on the surface of nanofibers. Human coronary artery endothelial cells (HCAECs) were seeded onto the blended nanofibers for viability, morphogenesis, attachment, and phenotypic studies. Five characteristic endothelial cell (EC) markers, including four types of cell adhesion molecule and one EC-preferential gene (von Willebrand factor), were studied by reverse transcription-polymerase chain reaction. Results showed that the collagen-blended polymer nanofibers could enhance the viability, spreading, and attachment of HCAECs and, moreover, preserve the EC phenotype. The blending electrospinning technique shows potential in refining the composition of polymer nanofibers by adding various ingredients (e.g., growth factors) according to cell types to fabricate tissue-engineering scaffold, particularly blood vessel-engineering scaffold.
authors.affiliation: Division of Bioengineering, National University of Singapore, Singapore. g0305833@nus.edu.sg
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Thomas
authors.initials: T
authors.lastname: Yong
authors.forename: Wee Eong
authors.initials: WE
authors.lastname: Teo
authors.forename: Zuwei
authors.initials: Z
authors.lastname: Ma
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D001673:Biodegradation, Environmental; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry*; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism; D018929:Cell Culture Techniques; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D003331:Coronary Vessels / Q000166:cytology; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D015870:Gene Expression; D006801:Humans; D008422:Materials Testing; D036103:Nanotechnology; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules; D003470:Culture Media; D011091:Polyesters; D003094:Collagen