Ozone sensitivity of currant tomato (Lycopersicon pimpinellifolium), a potential bioindicator species.
The wild tomato species Lycopersicon pimpinellifolium (currant tomato) was exposed to different O3 concentration, both in controlled environment fumigation facilities and in open-top chambers, to assess its sensitivity and to verify its potential as a bioindicator plant. Plants appeared particularly sensitive to O3 at an early stage of growth, responding with typical chlorotic spots within 24 h after exposure to a single pulse of 50 ppb for 3 h, and differentiating peculiar symptoms, such as reddish necrotic stipples, bronzing and extensive necrosis, depending on O3 concentration. Histo-cytochemical investigations with 3,3'-diaminobenzidine, to localize H2O2, and Evans blue, to detect dead cells, suggested that currant tomato sensitivity to O3 could be due to a deficiency in the anti-oxidant pools. The combination of these stainings proved to be useful, either to predict visible symptoms, early before their appearance, and to validate leaf ozone injury.
Iriti
Marcello
M
Istituto di Patologia Vegetale, Università di Milano, Via Celoria 2, 20133 Milan, Italy.
Belli
Lucia
L
Nali
Cristina
C
Lorenzini
Giacomo
G
Gerosa
Giacomo
G
Faoro
Franco
F
D015415:Biomarkers / Q000032:analysis; D016923:Cell Death / Q000187:drug effects; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods; D005651:Fumigation / Q000379:methods; D006861:Hydrogen Peroxide / Q000032:analysis; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000502:physiology; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D014026:Nicotiana / Q000187:drug effects
D015415:Biomarkers; D010083:Oxidants, Photochemical; D010126:Ozone; D006861:Hydrogen Peroxide