Identifying components of the NF-kappaB pathway in the beneficial Euprymna scolopes-Vibrio fischeri light organ symbiosis.
The Toll/NF-kappaB pathway is a common, evolutionarily conserved innate immune pathway that modulates the responses of animal cells to microbe-associated molecular patterns (MAMPs). Because MAMPs have been implicated as critical elements in the signaling of symbiont-induced development, an expressed sequence tag library from the juvenile light organ of Euprymna scolopes was used to identify members of the Toll/NF-kappaB pathway. Full-length transcripts were identified by using 5' and 3' RACE PCR. Seven transcripts critical for MAMP-induced triggering of the Toll/NF-kappaB phosphorylation cascade have been identified, including receptors, signal transducers, and a transcription factor. Further investigations should elucidate the role of the Toll/NF-kappaB pathway in the initiation of the beneficial symbiosis between E. scolopes and Vibrio fischeri.
Goodson
Michael S
MS
Department of Medical Microbiology and Immunology, University of Wisconsin, 1300 University Ave., Madison, WI 53706, USA.
Kojadinovic
Mila
M
Troll
Joshua V
JV
Scheetz
Todd E
TE
Casavant
Thomas L
TL
Soares
M Bento
MB
McFall-Ngai
Margaret J
MJ
D048908:Aliivibrio fischeri / Q000254:growth & development / Q000378:metabolism*; D000595:Amino Acid Sequence; D000825:Animal Structures / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D049832:Decapodiformes / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D020224:Expressed Sequence Tags / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008027:Light; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D009928:Organ Specificity; D015398:Signal Transduction; D013045:Species Specificity; D013559:Symbiosis; D014158:Transcription, Genetic
D018076:DNA, Complementary; D016328:NF-kappa B