title: Generation of biochemical response patterns of different substances using a whole cell assay with multiple signaling pathways.
abstract: Distinctive generation of biochemical response patterns of eight different substances, using an assay based on pigment containing cells, was demonstrated. Xenopus laevis melanophores, transfected with human beta(2)-adrenergic receptor, were seeded in a 96 well microplate and used to generate individual biochemical images through a two transient measuring protocol that contributes to highlight the response signatures of the agents. Adequate signal processing creates distinctive patterns in a time-concentration response space suitable for substance classification. The concept of biochemical images is introduced here. The assays were evaluated both with a standard microplate reader and with a computer screen photo-assisted technique (CSPT) yielding similar results. Since CSPT platforms only demand standard computer sets and web cameras as measuring setup, applications for these kind of assays outside main-laboratories were discussed.
authors.affiliation: Division of Applied Physics, Department of Physics and Measurement Technology, Linköping University, SE-581 83, Sweden. anksu@ifm.liu.se
authors.forename: Anke
authors.initials: A
authors.lastname: Suska
authors.forename: Daniel
authors.initials: D
authors.lastname: Filippini
authors.forename: Tony P M
authors.initials: TP
authors.lastname: Andersson
authors.forename: Ingemar
authors.initials: I
authors.lastname: Lundström
mesh_terms: D058672:Adrenergic beta-2 Receptor Antagonists; D000318:Adrenergic beta-Agonists / Q000008:administration & dosage* / Q000493:pharmacokinetics; D000818:Animals; D001671:Biochemistry / Q000379:methods; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D006728:Hormones / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008547:Melanophores / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D014982:Xenopus laevis
chemical_list: D058672:Adrenergic beta-2 Receptor Antagonists; D000318:Adrenergic beta-Agonists; D006728:Hormones; D018343:Receptors, Adrenergic, beta-2; D011994:Recombinant Proteins