title: Laboratory diagnosis of Ebola and Marburg hemorrhagic fever.
abstract: The control of Filovirus outbreaks can be greatly enhanced by timely laboratory confirmation of infection or the identification of alternative disease processes. The status of current laboratory diagnostics for Ebola and Marburg virus infections is discussed in terms of the assays available and their interpretation. In addition, the role of field-based laboratory support and its limitations and capabilities in an outbreak response setting, especially in regards to real-time PCR and immunofiltration assays, is presented.
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Grolla
authors.forename: A
authors.initials: A
authors.lastname: Lucht
authors.forename: D
authors.initials: D
authors.lastname: Dick
authors.forename: J E
authors.initials: JE
authors.lastname: Strong
authors.forename: H
authors.initials: H
authors.lastname: Feldmann
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002522:Chlorocebus aethiops; D029043:Ebolavirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D007159:Immunologic Tests / Q000379:methods*; D007391:International Cooperation; D008379:Marburg Virus Disease / Q000175:diagnosis*; D029024:Marburgvirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014709:Vero Cells / Q000821:virology; D014773:Virology / Q000379:methods*; D014776:Virus Cultivation
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012367:RNA, Viral