title: Adenosine A1 receptors mediate inhibition of cAMP formation in vitro in the pontine, REM sleep induction zone.
abstract: Microinjection of adenosine A1 receptor agonist or an inhibitor of adenylyl cyclase into the caudal, oral pontine reticular formation (PnOc) of the rat induces a long-lasting increase in REM sleep. Here, we report significant inhibition of forskolin-stimulated cAMP in dissected pontine tissue slices containing the PnOc incubated with the A1 receptor agonist, cyclohexaladenosine (10(-8) M). These data are consistent with adenosine A1 receptor agonist actions on REM sleep mediated through inhibition of cAMP.
authors.affiliation: Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Gerald.Marks@UTSouthwestern.edu
authors.forename: Gerald A
authors.initials: GA
authors.lastname: Marks
authors.forename: Christian G
authors.initials: CG
authors.lastname: Birabil
authors.forename: Samuel G
authors.initials: SG
authors.lastname: Speciale
mesh_terms: D000225:Adenine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D000241:Adenosine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D058916:Adenosine A1 Receptor Antagonists; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D066298:In Vitro Techniques; D008845:Microinjections; D010627:Phenethylamines / Q000008:administration & dosage / Q000494:pharmacology; D011149:Pons / Q000378:metabolism*; D051381:Rats; D020318:Rats, Long-Evans; D043682:Receptor, Adenosine A1 / Q000502:physiology*; D012895:Sleep, REM / Q000502:physiology*
chemical_list: D058916:Adenosine A1 Receptor Antagonists; D000067956:Adenylyl Cyclase Inhibitors; D004791:Enzyme Inhibitors; D010627:Phenethylamines; D043682:Receptor, Adenosine A1; C061282:2-(4-(2-carboxyethyl)phenethylamino)-5'-N-ethylcarboxamidoadenosine; C017759:9-(tetrahydro-2-furyl)-adenine; D005576:Colforsin; C027513:N(6)-cyclohexyladenosine; D000242:Cyclic AMP; D000225:Adenine; D000241:Adenosine