title: The packaging signal of MLV is an integrated module that mediates intracellular transport of genomic RNAs.
abstract: Packaging of MLV genomes requires four cis-acting stem-loops. Stem-loops A and B are self-complementary and bind Gag in their dimeric form, while the C and D elements mediate loop-loop interactions that facilitate RNA dimerization. Packaging also requires nuclear export of viral genomes, and their cytoplasmic transport toward the plasma membrane. For MLV, this is mediated by Gag and Env, and occurs on endosomal vesicles. Here, we report that MLV Psi acts at several steps during the transport of genomic RNAs. First, deletion of stem-loop B or C leads to the accumulation of genomic RNAs in the nucleus, suggesting that these elements are involved in export. Second, in chronically infected cells, mutation of the C and D loops impairs endosomal transport. This suggests that RNA dimerization is essential for vesicular transport, consistent with its proposed requirement for Gag binding. Surprisingly, deletion of stem-loop A blocks vesicular transport, whereas removal of stem-loop B has no effects. This suggests that stem-loop A has unique functions in packaging, not predicted from previous in vitro analyses. Finally, in packaging cells that do not express any Psi-containing RNA, endosomal RNA transport becomes sequence-independent. This non-specific activity of Gag likely promotes packaging of cellular mRNAs.
authors.affiliation: IGMM-CNRS UMR5535, IFR 24, 1919, route de Mende 34293 Montpellier Cedex 5, France.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Basyuk
authors.forename: Séverine
authors.initials: S
authors.lastname: Boulon
authors.forename: Finn
authors.initials: F
authors.lastname: Skou Pedersen
authors.forename: Edouard
authors.initials: E
authors.lastname: Bertrand
authors.forename: Søren
authors.initials: S
authors.lastname: Vestergaard Rasmussen
mesh_terms: D001483:Base Sequence; D001692:Biological Transport; D003593:Cytoplasm; D019281:Dimerization; D015686:Gene Products, env / Q000502:physiology; D015683:Gene Products, gag / Q000502:physiology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017384:Sequence Deletion; D015398:Signal Transduction; D019065:Virus Assembly
chemical_list: D015686:Gene Products, env; D015683:Gene Products, gag; D012367:RNA, Viral; D011994:Recombinant Proteins