The influence of genetic and environmental factors on plasma homocysteine concentrations in a population at high risk for coronary artery disease.
BACKGROUND
Elevated plasma total homocysteine (tHcy) predisposes to vascular disease and results from interactions between genetic and nutritional factors. MTHFR C(677)T increases tHcy in association with low folate. CBS 844ins68 lowers tHcy and negates the raising effect of MTHFR C(677)T in healthy subjects, but it is unclear if this is the case in subjects at high risk of vascular disease. This study examines the effect on plasma tHcy of interactions between these polymorphisms in an at-risk group.


METHODS
Blood samples were collected from 376 subjects at increased risk of coronary artery disease. Plasma tHcy and vitamin B(6) were measured by HPLC and red cell folate and serum vitamin B(12) were measured by immuno-luminometric assay. MTHFR C(677)T and CBS 844ins68 status was established by standard PCR techniques.


RESULTS
MTHFR TT predisposed to hyperhomocysteinaemia; this was increased in the presence of low folate (P<0.05) and vitamin B(12) (P<0.01). An inverse relationship was found between tHcy and folate (r=-0.42, P<0.0001), vitamin B(12) (r=-0.26, P<0.0005) and vitamin B(6) (r=-0.25, P<0.01). There was no interaction between plasma tHcy, vitamins or MTHFR C(677)T and CBS 844ins68.


CONCLUSIONS
In this population at high risk of coronary artery disease, plasma tHcy was determined by vitamin status. This was exacerbated by the MTHFR C(677)T mutation. CBS 844ins68 did not influence tHcy and did not negate the tHcy-raising effect of MTHFR C(677)T.
Bowron
Ann
A
Department of Clinical Biochemistry, Bristol Royal Infirmary, Bristol BS2 8HW, UK. ann.bowron@ubht.swest.nhs.uk
Scott
Jeff
J
Stansbie
David
D
D045103:5,10-Methylenetetrahydrofolate Reductase (FADH2) / Q000032:analysis / Q000097:blood / Q000235:genetics*; D003324:Coronary Artery Disease / Q000097:blood* / Q000235:genetics*; D003541:Cystathionine beta-Synthase / Q000097:blood / Q000235:genetics*; D004777:Environment; D004912:Erythrocytes / Q000737:chemistry; D005260:Female; D005492:Folic Acid / Q000032:analysis / Q000097:blood; D020022:Genetic Predisposition to Disease; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D012307:Risk Factors; D014805:Vitamin B 12 / Q000032:analysis / Q000097:blood; D025101:Vitamin B 6 / Q000032:analysis / Q000097:blood
D006710:Homocysteine; D025101:Vitamin B 6; D005492:Folic Acid; D045103:5,10-Methylenetetrahydrofolate Reductase (FADH2); D003541:Cystathionine beta-Synthase; D014805:Vitamin B 12