Assembly of plasmid DNA into liposomes after condensation by cationic lipid in anionic detergent solution.
A novel strategy to prepare negatively charged and small DNA-containing liposomes after condensation of plasmid DNA by a cationic lipid in deoxycholate micelle environment is described. The average diameter of resulting complexes was 62 +/- 8 nm. DNA-containing liposomes were then prepared by dialysis. The shape of the resulting liposomes was spherical. The average diameter and the surface charge of the liposomes were 86 +/- 6 nm and -24 +/- 3 mV, respectively. The plasmid DNA inside liposomes remained in a supercoiled form after incubation with DNase.
Zhang
Hong-Wei
HW
Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, West China School of Pharmacy, Sichuan University, Southern Renming Road, Chengdu, 610041, Sichuan, PR China.
Zhang
Ling
L
Sun
Xun
X
Diao
Shu
S
Zhang
Zhi-Rong
ZR
D002412:Cations / Q000737:chemistry; D004278:DNA, Superhelical / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008823:Micelles; D010957:Plasmids / Q000737:chemistry*
D002412:Cations; D004278:DNA, Superhelical; D003902:Detergents; D008081:Liposomes; D008823:Micelles