title: Molecular analysis of the simultaneous production of two SHV-type extended-spectrum beta-lactamases in a clinical isolate of Enterobacter cloacae by using single-nucleotide polymorphism genotyping.
abstract: Bacteria that simultaneously produce multiple extended-spectrum beta-lactamases are frequently isolated. We report an Enterobacter cloacae isolate, ES24, producing four different beta-lactamases (AmpC type beta-lactamase, TEM-1, SHV-7, and a novel extended-spectrum beta-lactamase, SHV-30). Direct sequencing of bla(SHV) gene products gave a "double peak" at position 703, suggesting the presence of more than one allele. Using fluorescence resonance energy transfer real-time PCR to detect single-nucleotide polymorphisms, we were able to distinguish two different bla(SHV) genes in a single isolate. This may prove to be a useful technique in surveys of beta-lactamase production in contemporary clinical isolates.
authors.affiliation: Institute of Medical Microbiology, Semmelweis University, Budapest Hungary.
authors.forename: Dóra
authors.initials: D
authors.lastname: Szabó
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Melan
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Hujer
authors.forename: Robert A
authors.initials: RA
authors.lastname: Bonomo
authors.forename: Kristine M
authors.initials: KM
authors.lastname: Hujer
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Bethel
authors.forename: Katalin
authors.initials: K
authors.lastname: Kristóf
authors.forename: David L
authors.initials: DL
authors.lastname: Paterson
mesh_terms: D016972:Enterobacter cloacae / Q000201:enzymology*; D005838:Genotype; D006801:Humans; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases