Prevention of thermal induced aggregation of cytochrome c at isoelectric pH values by polyanions.
Differential scanning calorimetry, viscometry, optical and CD spectroscopy were used to characterize the influence of two polyanions, poly(vinylsulfate) (PVS), and poly(4-styrene-sulfonate) (PSS) on thermal transition reversibility of ferricytochrome c at or near isoelectric pH. In these conditions, both PVS and PSS enhance the thermal transition reversibility of cytochrome c by preventing the aggregation of denatured protein molecules. Data indicate that the polyanions are in complex with cytochrome c that is stabilized by synergistic effect of Coulombic and non-Coulombic interactions.
Fedunová
Diana
D
Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences, Kosice.
Antalík
Marián
M
D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D045304:Cytochromes c / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids / Q000737:chemistry*; D014783:Viscosity
D045304:Cytochromes c; D000071228:Polyelectrolytes; D011108:Polymers; D011145:Polyvinyls; D013451:Sulfonic Acids; C019050:polyvinyl sulfate; C077114:styrenesulfonic acid polymer