The effect of vitamin A on CCl4-induced hepatic injuries in rats: a histochemical, immunohistochemical and ultrastructural study.
In this study, we aimed to investigate the effect of vitamin A on the transformation of the Ito cells to fibrogenic form and suppression of the development of fibrosis. Carbon tetrachloride intoxication was performed on rats for 2, 8, 12 or 20 weeks and 5x10(4) IU vitamin A (as retinol palmitate) was injected subcutaneously once every 4 weeks. Ito cells were detected by gold chloride impregnation, as well as desmin and alpha-smooth muscle actin (alpha-SMA) immunohistochemistry. Additionally, all groups were examined ultrastructurally. The number of Ito cells that were labelled positively with gold impregnation decreased in the fibrotic groups; however, alpha-SMA and desmin immunopositive Ito cells increased. The samples from animals that were treated with vitamin A showed an increase in labelling with gold impregnation but a decrease in alpha-SMA immunopositivity. The data showed that vitamin A can prevent hepatic injury, by suppressing the transformation of Ito cells to fibrogenic form. We conclude that vitamin A has potential for the treatment of hepatic fibrotic diseases. Alpha-SMA immunohistochemistry was found to be more informative than desmin immunohistochemistry for monitoring liver fibrosis.
Noyan
Semiha
S
Department of Histology and Embryology, Uludag University Faculty of Medicine, 16059, Gorukle, Bursa, Turkey. snoyan@uludag.edu.tr
Cavusoglu
Ilkin
I
Minbay
F Zehra
FZ
D000199:Actins / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D002251:Carbon Tetrachloride; D049109:Cell Proliferation / Q000187:drug effects; D003893:Desmin / Q000032:analysis; D017612:Gold Compounds / Q000032:analysis; D022781:Hepatocytes / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D006651:Histocytochemistry; D007150:Immunohistochemistry; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D014801:Vitamin A / Q000494:pharmacology / Q000627:therapeutic use*
D000199:Actins; D000975:Antioxidants; D002251:Carbon Tetrachloride; D003893:Desmin; D017612:Gold Compounds; D014801:Vitamin A; C498862:Acta2 protein, rat; C038016:gold chloride