title: Dietary palm olein oil augments cardiac antioxidant enzymes and protects against isoproterenol-induced myocardial necrosis in rats.
abstract: Wistar rats, 150-200 g, of either sex, were fed daily with commercial rat diet supplemented with palm olein oil in two doses (5% v/w (n = 16) and 10% v/w (n = 16) of diet) for 30 days. Control rats (n = 16) were fed with normal diet. On the 29(th) and 30(th) days, 8 rats from each group were administred isoproterenol (85 mg/kg, s.c., 24-h interval). On the 31(st) day, all rats were sacrificed and myocardial tissues were studied for thiobarbituric acid reactive substances (TBARS), antioxidant enzymes and light microscopic changes, along with the ferric-reducing ability of plasma (FRAP). A significant rise in myocardial superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) activity and FRAP level were observed in rats fed with palm olein oil. Isoproterenol caused an increase in myocardial oxidative stress in control rats, as evidenced by an increase in myocardial TBARS level, reduction in FRAP and myocardial SOD, catalase and GPx activity, along with focal necrosis of cardiac muscle fibres on light microscopy. The rise in myocardial TBARS and depletion of SOD and catalase activity following isoproterenol administration were prevented in palm-olein-oil-supplemented diet-fed rats at both doses. Isoproterenol-induced myocardial light-microscopic changes were also prevented in the treated groups. The results suggest that dietary palm olein oil caused augmentation of myocardial antioxidant enzymes and protected against isoproterenol-induced myocardial necrosis and associated oxidative stress.
authors.affiliation: Department of Pharmacology, All India Institute of Medical Sciences, New Delhi-110029, India.
authors.forename: D
authors.initials: D
authors.lastname: Narang
authors.forename: S
authors.initials: S
authors.lastname: Sood
authors.forename: M
authors.initials: M
authors.lastname: Thomas
authors.forename: A K
authors.initials: AK
authors.lastname: Dinda
authors.forename: S K
authors.initials: SK
authors.lastname: Maulik
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D004032:Diet; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D007545:Isoproterenol / Q000037:antagonists & inhibitors; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000201:enzymology* / Q000473:pathology; D009336:Necrosis / Q000517:prevention & control; D018384:Oxidative Stress / Q000187:drug effects*; D000073878:Palm Oil; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances; D013997:Time Factors
chemical_list: D004042:Dietary Fats, Unsaturated; D010938:Plant Oils; D017392:Thiobarbituric Acid Reactive Substances; D000073878:Palm Oil; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D007545:Isoproterenol