Simultaneous quantification of alpha-tocopherol and four major carotenoids in botanical materials by normal phase liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry.
A rapid and sensitive method for the simultaneous quantification of alpha-tocopherol, beta-carotene, beta-cryptoxanthin, lutein and zeaxanthin in botanical materials has been developed using normal phase liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry (NP-LC-APCI-MS/MS). A systematic extraction procedure for different botanical materials was described and the extraction solvents were matched with normal-phase LC requirement. Quantification was performed by using the external standards and standard calibration curves were linear between 35 and 8505 ng/mL for alpha-tocopherol, 64-15,552 ng/mL for beta-carotene, 40-9720 ng/mL for beta-cryptoxanthin, 70-17,010 ng/mL for lutein and 64-15,552 ng/mL for zeaxanthin with regression coefficient r2 > 0.9996. The limits of quantification (LOQ) were 35, 64, 40, 70 and 66 ng/mL for alpha-tocopherol, beta-carotene, beta-cryptoxanthin, lutein and zeaxanthin, respectively.
Hao
Zhigang
Z
Global Analytical Sciences Department, Colgate-Palmolive Company, Piscataway, NJ 08855, USA. zhigang.hao@gmail.com
Parker
Barry
B
Knapp
Michael
M
Yu
Liangli
L
D001274:Atmospheric Pressure; D002338:Carotenoids / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010938:Plant Oils / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014908:Triticum / Q000737:chemistry*; D024502:alpha-Tocopherol / Q000032:analysis*
D010938:Plant Oils; D002338:Carotenoids; D024502:alpha-Tocopherol