[Lethal anaphylactic shock model induced by human mixed serum in guinea pigs].
OBJECTIVE
To establish an anaphylactic shock model induced by human mixed serum in guinea pigs.


METHODS
Eighteen guinea pigs were divided into two groups: sensitized and control, The sensitized group were immunized intracutaneously with human mixed serum and then induced by endocardiac injection after 3 weeks.


RESULTS
Symptoms of anaphylactic shock appeared in the sensitized group. The level of serum IgE were increased in the sensitized group significantly.


CONCLUSIONS
An animal model of anaphylactic shock wer established successfully. It provide a tool for both forensic study and anaphylactic shock therapy.
Ren
Guang-Mu
GM
Department of forensic medicine,Shanxi Medical University, Taiyuan 030001, China. renguangmu@163.com
Bai
Ji-Wei
JW
Gao
Cai-Rong
CR
D000485:Allergens; D000707:Anaphylaxis / Q000097:blood / Q000276:immunology*; D000818:Animals; D003645:Death, Sudden; D004195:Disease Models, Animal; D005260:Female; D005554:Forensic Medicine; D006168:Guinea Pigs; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D011863:Radioimmunoassay; D011897:Random Allocation; D044967:Serum / Q000276:immunology*
D000485:Allergens; D007074:Immunoglobulin G