[Phylogenetic analysis of the surface glycoprotein genes of an aquatic bird origin influenza virus isolate A/Duck/Yangzhou/233/2002 (H6N2)].
Several H6 subtype avian A influenza viruses were isolated from aquatic birds in some live bird markets when we surveyed the ecology of the influenza in East China for more than two years and identified by specific RT-PCR. In this paper, the hemagglutinin (HA) and neuraminidase (NA) gene of one representative virus named A/Duck/Yangzhou/233/ 2002 (H6N2) (Dk/YZ/233/02) had been sequenced. Phylogenetic analysis of the H6 gene sequences available in the Influenza Sequence Database showed that the Dk/YZ/233/02 viruse cluster together with Dk/HK/3461/99 (H6N1) and Ck/Taiwan/na3/98 (H6N1). Phylogenetic analysis of the N2 NA genes of Dk/YZ/233/02 revealed that the NA gene of Dk/YZ/233/02 had genetically close relationships with that of H9N2 viruses isolated from duck in Japan and from chickens in South Korea, which were distinct from those of Ck/Beijing/94 (H9N2). The sequence of cleavage site between HA1 and HA2 of Dk/YZ/233/02 is P-Q-I-E-T-R-D, which was the typical characterization of the LPAIV.
Zhang
Ping-Hu
PH
Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China. zhangpinghu@163.com
Liu
Xiao-Wen
XW
Qian
Zhong-Ming
ZM
Tang
Ying-Hua
YH
Liu
Xiu-Fan
XF
D000818:Animals; D002645:Chickens; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics*; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics; D005585:Influenza in Birds / Q000821:virology*; D009439:Neuraminidase / Q000235:genetics*; D010802:Phylogeny
D019267:Hemagglutinin Glycoproteins, Influenza Virus; C109963:hemagglutinin, avian influenza A virus; D009439:Neuraminidase