Determination of urinary 8-hydroxy-2'-deoxyguanosine by two approaches-capillary electrophoresis and GC/MS: an assay for in vivo oxidative DNA damage in cancer patients.
8-Hydroxy-2'-deoxyguanosine (8OHdG) has been considered as an excellent marker of oxidative DNA damage associated with age-related diseases such as cancer. In this paper, two sensitive methods-capillary electrophoresis with electrochemical detection (CE-ECD) and gas chromatography/mass spectrometry (GC/MS) were developed for urinary 8OHdG analysis. The R.S.D. of the spiked recovery of the two methods for determining urinary 8OHdG was 4.03% and 8.25%, respectively, and the results from the two methods have a good consistency (r=0.999, P<0.01). The developed CE-ECD method was applied to investigate the urinary 8OHdG levels in different cancer patients and follow up the response of therapy. It was found that the excretion levels of urinary 8OHdG in cancer patients were significantly higher than those in healthy persons (35.26+/-27.96 nM versus 13.51+/-5.08 nM, P<0.05), and cancer patients receiving surgical therapy and chemotherapy showed a significant decrease in urinary 8OHdG.
Mei
Surong
S
National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China.
Yao
Qinghong
Q
Wu
Caiying
C
Xu
Guowang
G
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D004563:Electrochemistry / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000188:drug therapy / Q000601:surgery / Q000652:urine*; D015203:Reproducibility of Results
D000970:Antineoplastic Agents; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine