title: Higher plant-like fluorescence induction and thermoluminescence characteristics in cyanobacterium, Spirulina mutant defective in PQH2 oxidation by cytb6/f complex.
abstract: Characterization of the photosynthetic electron transport in a mutant of Spirulina platensis, generated by chemical mutagenesis, demonstrated that the electron transfer from the plastoquinone (PQ) to cytochrome b6/f was slowed. Thermoluminescence (TL) measurements suggested the presence of reversed energy flow via PQ, which resulted in an emergence of the plant-like after-glow TL band at 45 degrees C that could be enhanced by the transthylakoidal pH gradient and could be eliminated by an uncoupler, FCCP. The localization of the changes in the electron transport of the mutant cells measured by various methods revealed that the re-oxidation of the PQ pool is hampered in the mutant compared to the wild-type cells. The reduction in energy migration was localized between PQ and PS I reaction centers.
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Thung-kru, Bangkok, Thailand. marasri.rue@kmutt.ac.th
authors.forename: Marasri
authors.initials: M
authors.lastname: Ruengjitchatchawalya
authors.forename: László
authors.initials: L
authors.lastname: Kovács
authors.forename: Tipawan
authors.initials: T
authors.lastname: Mapaisansup
authors.forename: Anna
authors.initials: A
authors.lastname: Sallai
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Gombos
authors.forename: Mathurose
authors.initials: M
authors.lastname: Ponglikitmongkol
authors.forename: Morakot
authors.initials: M
authors.lastname: Tanticharoen
mesh_terms: D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D045346:Cytochrome b6f Complex / Q000378:metabolism*; D005453:Fluorescence; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D010788:Photosynthesis; D010971:Plastoquinone / Q000378:metabolism*
chemical_list: D045346:Cytochrome b6f Complex; D010971:Plastoquinone