title: Expression of functional G protein-coupled receptors in photoreceptors of transgenic Xenopus laevis.
abstract: G protein-coupled receptors (GPCRs) constitute the largest superfamily of transmembrane signaling proteins; however, the only known GPCR crystal structure is that of rhodopsin. This disparity reflects the difficulty in generating purified GPCR samples of sufficient quantity and quality. Rhodopsin, the light receptor of retinal rod neurons, is produced in large amounts of homogeneous quality in the vertebrate retina. We used transgenic Xenopus laevis to convert these retina rod cells into bioreactors to successfully produce 20 model GPCRs. The receptors accumulated in rod outer segments and were homogeneously glycosylated. Ligand and [(35)S]GTPgammaS binding assays of the 5HT(1A) and EDG(1) GPCRs confirmed that they were properly folded and functional. 5HT(1A)R was highly purified by taking advantage of the rhodopsin C-terminal immunoaffinity tag common to all GPCR constructs. We have also developed an automated system that can generate hundreds of transgenic tadpoles per day. This expression approach could be extended to other animal model systems and become a general method for the production of large numbers of GPCRs and other membrane proteins for pharmacological and structural studies.
authors.affiliation: Novasite Pharmaceuticals, Inc., San Diego, California 92121, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: David
authors.initials: D
authors.lastname: Salom
authors.forename: Jianhua
authors.initials: J
authors.lastname: He
authors.forename: Alex
authors.initials: A
authors.lastname: Okun
authors.forename: Juan
authors.initials: J
authors.lastname: Ballesteros
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Palczewski
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D006801:Humans; D008845:Microinjections / Q000295:instrumentation / Q000379:methods; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D049349:Receptors, Lysosphingolipid / Q000235:genetics / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017948:Retinal Rod Photoreceptor Cells / Q000166:cytology / Q000378:metabolism*; D019076:Transgenes; D014982:Xenopus laevis / Q000235:genetics / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D043562:Receptors, G-Protein-Coupled; D049349:Receptors, Lysosphingolipid; D011985:Receptors, Serotonin; D011993:Recombinant Fusion Proteins