Transcription factor GATA-3 is involved in repression of promoter activity of the human interleukin-4 gene.
GATA-3 was shown to bind to two sites of the IL-4 gene promoter in human T-cell lines PER-117 and Jurkat. A motif located in the region of position -860 and responsible for GATA-3 binding was detected for the first time. Mutation or deletion of this site increased the promoter activity. The findings suggest a direct involvement of GATA-3 in regulation of the human IL-4 gene transcription as a repressor of the promoter activity.
Schwenger
G T F
GT
Curtin University School of Biomedical Sciences and the Western Australian Institute of Medical Research, Molecular Immunology Group, Perth, 6000, Western Australia, Australia.
Mordvinov
V A
VA
Sanderson
C J
CJ
D001665:Binding Sites / Q000235:genetics; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics* / Q000378:metabolism; D019169:Jurkat Cells; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism; D014158:Transcription, Genetic
D016207:Cytokines; D050990:GATA3 Transcription Factor; D012097:Repressor Proteins; D015847:Interleukin-4