Preparation and characterization of SARS in-house reference antiserum.
A reference antiserum for SARS is in urgent need in the development of SARS vaccine and other serological test of SARS research. Convalescent serum was collected from clinical confirmed patient. ELISA, Western-blotting and neutralization assay detected specific antibody against SARS coronavirus. This antiserum was prepared as in-house reference antiserum, freeze-dried and sealed in ampoules. The potency of this reference antiserum is defined to be 52.7 U after extensive calibration. Further, collaborative studies for the evaluation of this serum are needed in order to satisfy the requirements for international reference antiserum.
Zhang
Jiansan
J
Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China. ZhangJS@Sinovac.com
Liu
Yuxuan
Y
Hu
Liangxiang
L
Gao
Qiang
Q
Zhang
Zhenshan
Z
Zhang
Xiaomei
X
Chen
Jiangting
J
Gong
Xuejie
X
Song
Lifei
L
Liu
Yufen
Y
Li
Jing
J
Li
Shufen
S
Huang
Jinfeng
J
Ning
Ye
Y
Gao
Hong
H
Qin
Chuan
C
Dong
Xiaoping
X
Wei
Jianguo
J
Dong
Guanmu
G
Yin
Weidong
W
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002522:Chlorocebus aethiops; D003289:Convalescence; D006801:Humans; D009500:Neutralization Tests; D012015:Reference Standards; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology* / Q000502:physiology; D012698:Serologic Tests / Q000592:standards; D045169:Severe Acute Respiratory Syndrome / Q000097:blood / Q000276:immunology*; D014709:Vero Cells
D000914:Antibodies, Viral