A solid supported reagent for internucleoside H-phosphonate linkage formation.
A fast and convenient procedure for synthesis of dinucleoside H-phosphonates is obtained through use of the novel polystyrene supported 5-carboxy-5-methyl-2-oxo-2-chloro-1,3,2-diaoxaphosphorinane reagent. Virtually quantitative H-phosphonate condensations are obtained leading to excellent isolatedyields and with only a simple filtration as the purification procedure. This provides for a convenient and high-yielding procedure that should be suited for solution-phase synthesis of oligonucleotides.
Mohe
Nikhil
N
Division of Organic and Bioorganic Chemistry, MBB, Scheele Laboratory, Karolinska Institutet, Stockholm, Sweden.
Heinonen
Petri
P
Sanghvi
Yogesh S
YS
Strömberg
Roger
R
D007202:Indicators and Reagents / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009705:Nucleosides / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D010755:Organophosphates / Q000737:chemistry; D063065:Organophosphonates / Q000737:chemistry*; D016877:Oxidants / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D007202:Indicators and Reagents; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D010755:Organophosphates; D063065:Organophosphonates; D016877:Oxidants