title: [The role of mitochondrial damages in Helicobacter pylori-induced apoptosis of gastric cancer cells].
abstract: OBJECTIVE
  To explain the role of mitochondrial pathway in the apoptosis of SGC-7901 cell line induced by concentrated Helicobacter pylori culture supernatant (CHCS).
  
  
  METHODS
  Cytochrome oxidase (COX) I expression was detected by Western blotting. Cell apoptosis and mitochondrial membrane potential were measured by flow cytometry.
  
  
  RESULTS
  CHCS could induce the apoptosis of SGC-7901 in a dose- and time-dependent manner. Apoptotic rates gradually enhanced followed by the concentrations increasing. The mitochondrial membrane potential (MMP) began to descend after treating CHCS for 4 h, and MMP descended most distinctly in 8 h. It descended the lowest point in 12 h, and it had no special changes in 24 h. The expression of COX I was notably lower than that of control group after CHCS treating (632.8 +/- 40.6 vs 895.1 +/- 44.2, P < 0.05).
  
  
  CONCLUSIONS
  Mitochondrial pathway may play an important role in the apoptosis of SGC-7901 cells induced by CHCS.
authors.affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Chun-hui
authors.initials: CH
authors.lastname: Lan
authors.forename: Dian-chun
authors.initials: DC
authors.lastname: Fang
authors.forename: Li-lin
authors.initials: LL
authors.lastname: Fan
authors.forename: Zhi-gao
authors.initials: ZG
authors.lastname: He
authors.forename: Dong-feng
authors.initials: DF
authors.lastname: Chen
authors.forename: Chong-yang
authors.initials: CY
authors.lastname: Liu
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Shi
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D003576:Electron Transport Complex IV / Q000378:metabolism; D005434:Flow Cytometry; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000473:pathology
chemical_list: D003576:Electron Transport Complex IV