Glutaraldehyde-induced occupational asthma: BALF components and BALF and serum Clara cell protein (CC16) changes due to specific inhalatory provocation test.
OBJECTIVE
The purpose of this study was to evaluate bronchoalveolar lavage fluid (BALF) components and Clara cell protein (CC16) concentration in serum and BALF in patients with glutaraldehyde (GA)-induced asthma, before and after a specific inhalatory provocation test (SIPT) with GA, in comparison to atopic asthmatics and healthy individuals.


METHODS
Spirometry and bronchoalveolar lavage were performed before and after SIPT. The serum and BALF concentrations of CC16 and cytogram content in BALF were evaluated.


RESULTS
In GA-sensitized asthmatics, the level of CC16 in BALF and serum was significantly lower at 24 h after SIPT in comparison with the values recorded prior to the experiment. There was a significant increase in the proportion of eosinophils, basophils and lymphocytes in BALF of GA-sensitized asthmatics obtained after SIPT.


CONCLUSIONS
The determination of CC16 either in serum or in BALF is a non-invasive test to detect Clara cell damage.
Palczynski
Cezary
C
Department of Occupational Diseases, Centre of Occupational and Environmental Allergy, Nofer Institute of Occupational Medicine, 8 Teresy St, 90-950 Lodz, Poland.
Walusiak
Jolanta
J
Krakowiak
Anna
A
Halatek
Tadeusz
T
Ruta
Urszula
U
Krawczyk-Adamus
Patrycja
P
Wittczak
Tomasz
T
Swiercz
Radoslaw
R
Gorski
Pawel
P
Rydzynski
Konrad
K
D000328:Adult; D001249:Asthma / Q000139:chemically induced* / Q000175:diagnosis / Q000276:immunology; D001491:Basophils; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000276:immunology; D016022:Case-Control Studies; D019742:Chemokines, CC / Q000032:analysis* / Q000097:blood; D004804:Eosinophils; D005260:Female; D005976:Glutaral / Q000633:toxicity*; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis / Q000209:etiology / Q000276:immunology; D007958:Leukocyte Count; D008168:Lung / Q000503:physiopathology; D008214:Lymphocytes; D009784:Occupational Diseases / Q000139:chemically induced* / Q000175:diagnosis / Q000276:immunology; D013147:Spirometry
D015415:Biomarkers; D019742:Chemokines, CC; C070959:Ccl6 protein, mouse; D005976:Glutaral