CgDN24: a gene involved in hyphal development in the fungal phytopathogen Colletotrichum gloeosporioides.
A cDNA corresponding to a transcript induced in culture by N starvation, was identified in Colletotrichum gloeosporioides by a differential hybridisation strategy. The cDNA comprised 905 bp and predicted a 215 aa protein; the gene encoding the cDNA was termed CgDN24. No function for CgDN24 could be predicted by database homology searches using the cDNA sequence and no homologues were found in the sequenced fungal genomes. Transcripts of CgDN24 were detected in infected leaves of Stylosanthes guianensis at stages of infection that corresponded with symptom development. The CgDN24 gene was disrupted by homologous recombination and this led to reduced radial growth rates and the production of hyphae with a hyperbranching phenotype. Normal sporulation was observed, and following conidial inoculation of S. guianensis, normal disease development was obtained. These results demonstrate that CgDN24 is necessary for normal hyphal development in axenic culture but dispensable for phytopathogenicity.
Stephenson
Sally-Anne
SA
Cooperative Research Centre for Tropical Plant Pathology, The University of Queensland, Brisbane 4072, Australia.
Stephens
Camilla M
CM
Maclean
Donald J
DJ
Manners
John M
JM
D020231:Colletotrichum / Q000235:genetics* / Q000254:growth & development*; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D018628:Gene Dosage; D005800:Genes, Fungal; D025301:Hyphae / Q000235:genetics / Q000254:growth & development*; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics* / Q000502:physiology*; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D018515:Plant Leaves / Q000382:microbiology; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013172:Spores, Fungal
D018076:DNA, Complementary; D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger