N-aryl-9-amino-substituted acridizinium derivatives as fluorescent "light-up" probes for DNA and protein detection.
[reaction: see text] N-Arylamino-substituted acridizinium (benzo[b]quinolizinium) derivatives are almost nonfluorescent in water or organic solvents; however, upon addition of calf thymus DNA or bovine serum albumin the fluorescence intensity increases by a factor of 10 to 50. Thus, these dyes exhibit ideal properties to be used as DNA- and protein-sensitive "light-up probes".
Granzhan
Anton
A
Institut für Organische Chemie, Universität Siegen, Adolf-Reichwein-Strasse 2, D-57068 Siegen, Germany.
Ihmels
Heiko
H
D000166:Acridines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
D000166:Acridines; D005456:Fluorescent Dyes; D011506:Proteins; D012710:Serum Albumin, Bovine; D004247:DNA; C427203:calf thymus DNA