Microfluidic device for electric field-driven single-cell capture and activation.
A microchip that performs directed capture and chemical activation of surface-modified single cells has been developed. The cell capture system is comprised of interdigitated gold electrodes microfabricated on a glass substrate within PDMS channels. The cell surface is labeled with thiol functional groups using endogenous RGD receptors, and adhesion to exposed gold pads on the electrodes is directed by applying a driving electric potential. Multiple cell types can thus be sequentially and selectively captured on desired electrodes. Single-cell capture efficiency is optimized by varying the duration of field application. Maximum single-cell capture is attained for the 10-min trial, with 63 +/- 9% (n = 30) of the electrode pad rows having a single cell. In activation studies, single M1WT3 CHO cells loaded with the calcium-sensitive dye fluo-4 AM were captured; exposure to the muscarinic agonist carbachol increased the fluorescence to 220 +/- 74% (n = 79) of the original intensity. These results demonstrate the ability to direct the adhesion of selected living single cells on electrodes in a microfluidic device and to analyze their response to chemical stimuli.
Toriello
Nicholas M
NM
Department of Chemistry and UC Berkeley/UCSF Joint Graduate Group in Bioengineering, University of California, Berkeley, California 94720, USA.
Douglas
Erik S
ES
Mathies
Richard A
RA
D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002469:Cell Separation / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods