Early loss of proliferative potential of human peritoneal mesothelial cells in culture: the role of p16INK4a-mediated premature senescence.
Much has been learned about the mechanisms underlying cellular senescence. The pathways leading to senescence appear to vary, depending on the cell type and cell culture conditions. In this respect, little is known about senescence of human peritoneal mesothelial cells (HPMC). Previous studies have significantly differed in the reported proliferative lifespan of HPMC. Therefore, in the present study, we have examined how HPMC enter state of senescence under conditions typically used for HPMC culture. HPMC were isolated from omentum and grown into senescence. The cultures were assessed for the growth rate, the presence of senescence markers, activation of cell-cycle inhibitors, and the oxidative stress. HPMC were found to reach, on average, six population doublings before senescence. The terminal growth arrest was associated with decreased expression of Ki67 antigen, increased percentage of cells in the G1 phase, reduced early population doubling level cDNA-1 mRNA expression, and the presence of senescence-associated beta-galactosidase. Compared with early-passage cells, the late-passage HPMC exhibited increased expression of p16INK4a but not of p21Cip1. In addition, these cells generated more reactive oxygen species and displayed increased presence of oxidatively modified DNA (8-hydroxy-2'-deoxyguanosine). These results demonstrate that early onset of senescence in omentum-derived HPMC may be associated with oxidative stress-induced upregulation of p16INK4a.
Ksiazek
Krzysztof
K
Dept. of Pathophysiology, Univ. Medical School, Swiecickiego 6, 60-781 Poznań, Poland.
Piwocka
Katarzyna
K
Brzezińska
Agnieszka
A
Sikora
Ewa
E
Zabel
Maciej
M
Breborowicz
Andrzej
A
Jörres
Achim
A
Witowski
Janusz
J
D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics / Q000502:physiology*; D004247:DNA / Q000032:analysis; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D016193:G1 Phase / Q000502:physiology; D005786:Gene Expression Regulation; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis / Q000235:genetics / Q000502:physiology; D009852:Omentum / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D018384:Oxidative Stress; D010537:Peritoneum / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015238:Sulfotransferases; D001616:beta-Galactosidase / Q000032:analysis
D002135:Calcium-Binding Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D019394:Ki-67 Antigen; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D004247:DNA; D015238:Sulfotransferases; C028152:alcohol sulfotransferase; D001616:beta-Galactosidase