Matrix-assisted laser desorption/ionization mass spectrometry of taxanes.
Taxanes are biologically active compounds that have been extensively used in pharmacology for their powerful anticancer properties. High specificity and low level sensitivity for analysis of these compounds have been obtained with reversed-phase high-pressure liquid chromatography/mass spectrometry (RP-HPLC/MS), but the number of applications of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for low molecular weight analytes is rapidly growing. A new MALDI-MS approach for the rapid screening of a variety of taxanes and a tandem mass spectrometric (MS/MS) analysis of the most important and diagnostic taxane fragmentation pathways are proposed. A solid-phase extraction method followed by preliminary quantification is also reported.
Armirotti
Andrea
A
Department of Experimental Medicine and Center of Excellence for Biomedical Research, University of Genoa, Viale Benedetto XV 7, 16132 Genova, Italy. andrea.armirotti@unige.it
Benatti
Umberto
U
Miele
Mariangela
M
Damonte
Gianluca
G
D000470:Alkaloids / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry*; D017239:Paclitaxel / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D043823:Taxoids / Q000737:chemistry*
D000470:Alkaloids; D000970:Antineoplastic Agents; D043823:Taxoids; C073950:baccatin III; D017239:Paclitaxel