title: [Effect of salidroside on mitochondria injury induced by sodium azide].
abstract: OBJECTIVE
  To study the protective effect of salidroside on mitochondria injury induced by sodium azide.
  
  
  METHODS
  Human neuroblastoma SH-SY5Y cells were exposed to sodium azide with different concentration of salidroside, then cell viability was measured by thiazolyl blue (MTT) method and mitochondrial membrane potential (MMP) was detected by JC-1 method. Protective effect of salidroside against disfunction of mitochondria induced by sodium azide was detected by resazurin method.
  
  
  RESULTS
  After exposing to 64 mmol x L(-1) sodium azide for 4 hours, cell viability and MMP of SH-SY5Y cells significantly decreased. When pretreated with salidroside, the cell damage was greatly reduced and the mitochondrial membrane potential was maintained. Furthermore, salidroside can protect function of rat brain mitochondria against damage induced by sodium azide.
  
  
  CONCLUSIONS
  Salidroside was demonstrated to play an important role in improving the function of mitochondria.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Cao
authors.forename: Guan-hua
authors.initials: GH
authors.lastname: Du
authors.forename: Min-wei
authors.initials: MW
authors.lastname: Wang
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology* / Q000503:physiopathology; D045744:Cell Line, Tumor; D002470:Cell Survival; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009447:Neuroblastoma / Q000473:pathology* / Q000503:physiopathology; D010084:Oxidation-Reduction / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D029771:Rhodiola / Q000737:chemistry; D019810:Sodium Azide
chemical_list: D005960:Glucosides; D010636:Phenols; D019810:Sodium Azide; C009172:rhodioloside