title: Pharmacological studies of anti-diarrhoeal activity of Gentianopsis paludosa.
abstract: The pharmacological studies of anti-diarrhoeal activity of Gentianopsis paludosa were carried out. The 75% ethanol extract (100-400 mg/kg) of Gentianopsis paludosa inhibited castor oil-induced diarrhoea and also reduced gastrointestinal motility in the charcoal meal test in mice. In the rabbit-isolated ileum, the extract showed inhibitory effects not only on its spontaneous contraction, but also on acetylcholine (Ach, 0.1 mM)- and KCl (60 mM)-induced contractions. Furthermore, the ethanol extract (0.4-0.6 mg/ml) shifted the CaCl2 dose-effect curves to the right in a non-competitive manner and significantly reduced the acetylcholine-induced contraction of isolated ileum in Ca2+-free solution like verapamil (0.1 mM), which suggest that the extract interferes with Ca2+ movement. These results support the traditional use of Gentianopsis paludosa in the treatment of diarrhoea.
authors.affiliation: The Natural Products and Glycoconjugate Research Group 1805, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. whd77@dicp.ac.cn
authors.forename: Huandi
authors.initials: H
authors.lastname: Wang
authors.forename: Chengyu
authors.initials: C
authors.lastname: Tan
authors.forename: Xuefang
authors.initials: X
authors.lastname: Bai
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Du
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000930:Antidiarrheals / Q000008:administration & dosage / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005769:Gastrointestinal Motility / Q000187:drug effects; D019684:Magnoliopsida / Q000737:chemistry*; D051379:Mice; D011189:Potassium Chloride / Q000494:pharmacology; D011817:Rabbits
chemical_list: D000930:Antidiarrheals; D011189:Potassium Chloride; D002122:Calcium Chloride; D000109:Acetylcholine