Use of an efflux-deficient streptococcus pneumoniae strain panel to identify ABC-class multidrug transporters involved in intrinsic resistance to antimicrobial agents.
Thirteen derivatives of the Streptococcus pneumoniae TIGR4 strain in which putative drug efflux pumps were genetically inactivated were constructed and characterized. The results indicate that two linked genes encoding the ABC-type transporters SP2073 and SP2075 function together to confer intrinsic resistance to a series of structurally unrelated compounds, including certain fluoroquinolones.
Robertson
Gregory T
GT
Cumbre, Inc., 1502 Viceroy Dr., Dallas, Texas 75235-2304, USA.
Doyle
Timothy B
TB
Lynch
A Simon
AS
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000502:physiology*; D024881:Drug Resistance, Bacterial; D018432:Drug Resistance, Multiple; D008826:Microbial Sensitivity Tests; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins