title: Assembly of plasmid DNA into liposomes after condensation by cationic lipid in anionic detergent solution.
abstract: A novel strategy to prepare negatively charged and small DNA-containing liposomes after condensation of plasmid DNA by a cationic lipid in deoxycholate micelle environment is described. The average diameter of resulting complexes was 62 +/- 8 nm. DNA-containing liposomes were then prepared by dialysis. The shape of the resulting liposomes was spherical. The average diameter and the surface charge of the liposomes were 86 +/- 6 nm and -24 +/- 3 mV, respectively. The plasmid DNA inside liposomes remained in a supercoiled form after incubation with DNase.
authors.affiliation: Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, West China School of Pharmacy, Sichuan University, Southern Renming Road, Chengdu, 610041, Sichuan, PR China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Xun
authors.initials: X
authors.lastname: Sun
authors.forename: Shu
authors.initials: S
authors.lastname: Diao
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Zhang
mesh_terms: D002412:Cations / Q000737:chemistry; D004278:DNA, Superhelical / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008823:Micelles; D010957:Plasmids / Q000737:chemistry*
chemical_list: D002412:Cations; D004278:DNA, Superhelical; D003902:Detergents; D008081:Liposomes; D008823:Micelles