title: Oral administration of a decaffeinated green tea (Camellia sinensis) extract did not alter urinary 8-epi-prostaglandin F(2 alpha), a biomarker for in-vivo lipid peroxidation.
abstract: Oxidative stress is involved in the pathogenesis of numerous chronic human diseases. The objective of this study was to determine whether administration of a decaffeinated green tea extract providing 844 mg flavonoids daily reduced the urinary excretion of 8-epi-prostaglandin F(2 alpha) (8-epi-PGF(2 alpha)), a product of lipid peroxidation in cellular membranes and of low-density lipoprotein (LDL). Nine healthy male and female subjects were studied at baseline and after 14 days of green tea supplementation. Analysis of urinary 8-epi-PGF(2 alpha) was performed using immunoaffinity extraction-gas chromatography-negative ion chemical ionization-mass spectrometry (GC-NICI-MS). Urinary 8-epi-PGF(2 alpha) concentrations were 0.286+/-0.120 nmol (mmol creatinine)(-1) at baseline and 0.244+/-0.177 nmol mmol(-1) creatinine after green tea supplementation. There were no significant differences in the excretion of urinary 8-epi-PGF(2 alpha) after treatment with green tea. We conclude that 14 days of green tea supplementation did not significantly alter in-vivo lipid peroxidation.
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, SC 29425, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Donovan
authors.forename: C Lindsay
authors.initials: CL
authors.lastname: DeVane
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Chavin
authors.forename: James C
authors.initials: JC
authors.lastname: Oates
authors.forename: Chinedu
authors.initials: C
authors.lastname: Njoku
authors.forename: Kennerly S
authors.initials: KS
authors.lastname: Patrick
authors.forename: Ryan N
authors.initials: RN
authors.lastname: Fiorini
authors.forename: John S
authors.initials: JS
authors.lastname: Markowitz
mesh_terms: D000284:Administration, Oral; D000328:Adult; D015415:Biomarkers / Q000737:chemistry / Q000652:urine; D028241:Camellia sinensis / Q000737:chemistry*; D002214:Capsules; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood / Q000493:pharmacokinetics / Q000494:pharmacology / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003404:Creatinine / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D013662:Tea / Q000737:chemistry*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D002214:Capsules; D005419:Flavonoids; D010936:Plant Extracts; D013662:Tea; C075750:8-epi-prostaglandin F2alpha; D002392:Catechin; D003404:Creatinine; D015237:Dinoprost; C045651:epigallocatechin gallate