Terminal phosphate labeled nucleotides: synthesis, applications, and linker effect on incorporation by DNA polymerases.
A number of terminal phosphate-labeled nucleotides with three or more phosphates and with varied length linkers attached between the terminal phosphate and the dye have been synthesized. These nucleotides have been tested as substrates for different DNA and RNA polymerases. We have also explored their utility in DNA sequencing, SNP analysis, nucleic acid amplification, quantitative PCR, and other biochemical assays.
Kumar
Shiv
S
GE Healthcare, 800 Centennial Ave., Piscataway, NJ 08855-1327, USA.
Sood
Anup
A
Wegener
Jeffery
J
Finn
Patrick J
PJ
Nampalli
Satyam
S
Nelson
John R
JR
Sekher
Anuradha
A
Mitsis
Paul
P
Macklin
John
J
Fuller
Carl W
CW
D004396:Coloring Agents / Q000494:pharmacology; D004247:DNA / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D005821:Genetic Techniques; D008956:Models, Chemical; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D013997:Time Factors
D004396:Coloring Agents; D017931:DNA Primers; D009711:Nucleotides; D010710:Phosphates; D004247:DNA; D004259:DNA-Directed DNA Polymerase