Further evaluation of the skin micronucleus test: results obtained using 10 polycyclic aromatic hydrocarbons.
The standard in vivo micronucleus (MN) test detects clastogenicity in hematopoietic cells and is not suitable for detecting chemicals that target the skin. Previously, we have developed an in vivo rodent skin MN test that is simple to perform and can be applied to several laboratory animals, including the hairless mouse-a species whose use simplifies the procedure of skin testing. In this paper, we report new data that confirms the predictive ability of the test. Following the application of 10 polycyclic aromatic hydrocarbons (7,12-dimethylbenz[a]anthracene; 3-methylcholanthrene; benzo[a]pyrene; dibenz[a,h]anthracene; benz[a]anthracene; dibenz[a,c]anthracene; chrysene; benzo[e]pyrene; pyrene; anthracene) with various degrees of genotoxicity to the dorsal skin of hairless mice, we found that these compounds caused MN production that in general correlated with their reported carcinogenicity. We believe that this test will be useful in detecting skin clastogens that test negative when analyzed using the standard micronucleus test.
Nishikawa
Takashi
T
Human Safety Evaluation Center, Lion, 100 Tajima, Odawara, Kanagawa 256-0811, Japan. ntaka@lion.co.jp
Nakamura
Tsuneaki
T
Fukushima
Akira
A
Takagi
Yutaka
Y
D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D051379:Mice; D008812:Mice, Hairless; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests / Q000379:methods*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D012867:Skin / Q000187:drug effects*
D002273:Carcinogens; D011084:Polycyclic Aromatic Hydrocarbons