[The molecular mechanism of PML in inducing apoptosis of bladder cancer cell line].
OBJECTIVE
Study the affection of inducing expression of PML in the apoptosis and the molecular mechanism of bladder cancer.


METHODS
PMEP4/PML inducible expression vector was transfected into bladder cancer UM-UC-2 cells by lipofectamine 2000 system. The positive clone cells were selected by 300 microg/ml hygromycin B and confirmed by laser confocal imaging system. Then, using the in vitro DNA ladder apoptotic assay and Western blot, the affection of inducing expression of PML on apoptosis and its molecular mechanism of bladder cancer cell was studied.


RESULTS
Comparing with the vector control group, PML specific nuclear speckle significantly increased in the PMEP4/PML bladder cells. DNA ladder assay demonstrated bladder cancer cell expressing PML occurred apoptosis while the control vector cells were not influenced. Overexpression of PML could reduce Survivin expression and upregulate caspase3 and cleaved PARP protein expression


CONCLUSIONS
Our results demonstrated that overexpression of PML could induce bladder cancer cell apoptosis through the caspase dependent pathways.
Li
Lei
L
Institute of Urology, No.1 Hospital, Xi'an Jiaotong University, Shanxi 710061, China.
He
Da-lin
DL
He
Hui
H
Wang
Xin-yang
XY
Zhang
Lin-lin
LL
Luo
Yong
Y
Nan
Xun-yi
XY
D017209:Apoptosis / Q000502:physiology*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology*
D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human