Protein-coated microcrystals for use in organic solvents: application to oxidoreductases.
Protein-coated microcrystals (PCMC), a biocatalyst preparation previously demonstrated to display substantially increased transesterification activity of proteases and lipases in organic solvents when compared to their as received counterparts [Kreiner M, Moore BD, Parker MC (2001) Chem. Commun. 12:1096--1097], was applied to oxidoreductases. Horse liver alcohol dehydrogenase (HLADH), catalase (CAT), soybean peroxidase and horseradish peroxidase were immobilised onto K(2)SO(4) crystals and dehydrated in a 1-step process, resulting in PCMC. These PCMC preparations showed enhanced activity in different organic solvents in most types of reactions tested. The highest activation was observed with HLADH (50-fold as active as enzyme as received) and CAT (25-fold).
Kreiner
Michaela
M
Department of Chemistry, University of Glasgow, Joseph Black Building, G12 8QQ, Glasgow, UK.
Parker
Marie-Claire
MC
D000818:Animals; D002384:Catalysis; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry*; D010088:Oxidoreductases / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry*
D004800:Enzymes, Immobilized; D012997:Solvents; D013431:Sulfates; C031512:potassium sulfate; D010088:Oxidoreductases