The accumulation of abundant soluble proteins changes early in the development of the primary roots of maize (Zea mays L.).
A reference database of the major soluble proteins of the primary root of the maize inbred line B73 was generated 5 days after germination (DAG) using a combination of 2-DE and MALDI-TOF MS. A total of 302 protein spots were detected with CBB in a pH 4-7 range and 81 proteins representing 74 distinct Genbank accessions were identified. Only 28% of the major proteins identified in 5 DAG primary roots were identified in similarly analyzed 9 DAG primary roots documenting remarkable changes in the accumulation of abundant soluble proteins early in primary root development.
Hochholdinger
Frank
F
ZMBP, Center for Plant Molecular Biology, University of Tuebingen, Auf der Morgenstelle 28, 72076 Tuebingen, Germany. frank.hochholdinger@zmbp.uni-tuebingen.de
Woll
Katrin
K
Guo
Ling
L
Schnable
Patrick S
PS
D015180:Electrophoresis, Gel, Two-Dimensional; D010940:Plant Proteins / Q000096:biosynthesis*; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism*; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D003313:Zea mays / Q000378:metabolism*
D010940:Plant Proteins