title: The rapid evaluation of bacterial growth in blood cultures by selected ion flow tube-mass spectrometry (SIFT-MS) and comparison with the BacT/ALERT automated blood culture system.
abstract: We compared the performance of the BacT/ALERT automated blood culture system with real-time, quantitative volatile organic compound (VOC) detection by selected ion flow tube-mass spectrometry (SIFT-MS). Blood samples from healthy donors were artificially infected with 5 or 100 CFU of organisms commonly causing bacteremia. Positive results by SIFT-MS analysis of headspace gases were recorded for 53/60 (88.3%) bottles at 8h, and 58/60 (96.6%) bottles at 24 h. We conclude that SIFT-MS is a sensitive method for the detection of microbial VOCs. Furthermore, profiles of the VOCs detected may allow simultaneous identification of infecting organisms.
authors.affiliation: Syft Technologies Ltd., Medical Applications, Christchurch, New Zealand. jenny.scotter@syft.com
authors.forename: J M
authors.initials: JM
authors.lastname: Scotter
authors.forename: R A
authors.initials: RA
authors.lastname: Allardyce
authors.forename: V S
authors.initials: VS
authors.lastname: Langford
authors.forename: A
authors.initials: A
authors.lastname: Hill
authors.forename: D R
authors.initials: DR
authors.lastname: Murdoch
mesh_terms: D001331:Automation; D016470:Bacteremia / Q000382:microbiology*; D001419:Bacteria / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D013997:Time Factors
chemical_list: D003470:Culture Media