The rapid evaluation of bacterial growth in blood cultures by selected ion flow tube-mass spectrometry (SIFT-MS) and comparison with the BacT/ALERT automated blood culture system.
We compared the performance of the BacT/ALERT automated blood culture system with real-time, quantitative volatile organic compound (VOC) detection by selected ion flow tube-mass spectrometry (SIFT-MS). Blood samples from healthy donors were artificially infected with 5 or 100 CFU of organisms commonly causing bacteremia. Positive results by SIFT-MS analysis of headspace gases were recorded for 53/60 (88.3%) bottles at 8h, and 58/60 (96.6%) bottles at 24 h. We conclude that SIFT-MS is a sensitive method for the detection of microbial VOCs. Furthermore, profiles of the VOCs detected may allow simultaneous identification of infecting organisms.
Scotter
J M
JM
Syft Technologies Ltd., Medical Applications, Christchurch, New Zealand. jenny.scotter@syft.com
Allardyce
R A
RA
Langford
V S
VS
Hill
A
A
Murdoch
D R
DR
D001331:Automation; D016470:Bacteremia / Q000382:microbiology*; D001419:Bacteria / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods; D001769:Blood / Q000382:microbiology*; D003470:Culture Media; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D013997:Time Factors
D003470:Culture Media