Proteomic analysis of protein components in periodontal ligament fibroblasts.
BACKGROUND
Characterization of periodontal ligament (PDL) fibroblast proteome is an important tool for understanding PDL physiology and regulation and for identifying disease-related protein markers. PDL fibroblast protein expression has been studied using immunological methods, although limited to previously identified proteins for which specific antibodies are available.


METHODS
We applied proteomic analysis coupled with mass spectrometry and database knowledge to human PDL fibroblasts.


RESULTS
We detected 900 spots and identified 117 protein spots originating in 74 different genes. In addition to scaffold cytoskeletal proteins, e.g., actin, tubulin, and vimentin, we identified proteins implicated with cellular motility and membrane trafficking, chaparonine, stress and folding proteins, metabolic enzymes, proteins associated with detoxification and membrane activity, biodegradative metabolism, translation and transduction, extracellular proteins, and cell cycle regulation proteins.


CONCLUSIONS
Most of these identified proteins are closely related to the extensive PDL fibroblasts' functions and homeostasis. Our PDL fibroblast proteome map can serve as a reference map for future clinical studies as well as basic research.
Reichenberg
E
E
Institute of Dental Sciences, Hadassah Faculty of Dental Medicine, The Hebrew University, Jerusalem, Israel.
Redlich
M
M
Cancemi
P
P
Zaks
B
B
Pitaru
S
S
Fontana
S
S
Pucci-Minafra
I
I
Palmon
A
A
D000293:Adolescent; D002478:Cells, Cultured; D002648:Child; D003598:Cytoskeletal Proteins / Q000032:analysis; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D010449:Peptide Mapping / Q000379:methods*; D010513:Periodontal Ligament / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000502:physiology; D020543:Proteome / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003598:Cytoskeletal Proteins; D011506:Proteins; D020543:Proteome