title: Micropatterned cell co-cultures using layer-by-layer deposition of extracellular matrix components.
abstract: Micropatterned cellular co-cultures were fabricated using three major extracellular matrix components: hyaluronic acid (HA), fibronectin (FN) and collagen. To fabricate co-cultures with these components, HA was micropatterned on a glass substrate by capillary force lithography, and the regions of exposed glass were coated with FN to generate cell adhesive islands. Once the first cell type was immobilized on the adhesive islands, the subsequent electrostatic adsorption of collagen to HA patterns switched the non-adherent HA surfaces to adherent, thereby facilitating the adhesion of a second cell type. This technique utilized native extracellular matrix components and therefore affords high biological affinity and no cytotoxicity. This biocompatible co-culture system could potentially provide a new tool to study cell behavior such as cell-cell communication and cell-matrix interactions, as well as tissue-engineering applications.
authors.affiliation: Department of Chemical Engineering, Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Junji
authors.initials: J
authors.lastname: Fukuda
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
authors.forename: Judy
authors.initials: J
authors.lastname: Yeh
authors.forename: George
authors.initials: G
authors.lastname: Eng
authors.forename: Jianjun
authors.initials: J
authors.lastname: Cheng
authors.forename: Omid C
authors.initials: OC
authors.lastname: Farokhzad
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D018920:Coculture Techniques / Q000295:instrumentation; D003094:Collagen / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005898:Glass; D022781:Hepatocytes / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D011107:Polylysine; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D011107:Polylysine; D006820:Hyaluronic Acid; D003094:Collagen