NO-donors, part X [1] : investigations on the stability of pentaerythrityl tetranitrate (PETN) by HPLC-chemoluminescence-N-detection (CLND) versus UV-detection in HPLC.
HPLC in combination with chemoluminescence-N-detection (CLND) is very useful for the analysis of pentaerythrityl tetranitrate (PETN) and its possible biological and chemical degradation products pentaerythrityl trinitrate (PETriN), pentaerythrityl dinitrate (PEDiN) and pentaerythrityl mononitrate (PEMonoN). Quantification is more convenient and sensitivity of this method is about four times higher compared to UV-detection. The present study demonstrates that PETN is a chemically more stable compound in vitro than expected. No degradation was observed in aqueous buffers (37 degrees C, pH 5.6, 7.4), human plasma, and simulated intestinal or gastric fluid. On the other hand, the addition of increasing amounts of thioles (cysteine, thioglycolic acid) induced an increasing degradation of PETN.
Seeling
Andreas
A
Institute of Pharmacy, Friedrich-Schiller-University Jena, Philosophenweg 14, D-07743 Jena, Germany. b8sean@rz.uni-jena.de
Lehmann
Jochen
J
D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D008163:Luminescent Measurements; D020030:Nitric Oxide Donors / Q000737:chemistry*; D010084:Oxidation-Reduction; D010417:Pentaerythritol Tetranitrate / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D020030:Nitric Oxide Donors; D012996:Solutions; D012997:Solvents; D010417:Pentaerythritol Tetranitrate