Transient isolation of Burkholderia multivorans and Burkholderia cenocepacia from a Brazilian cystic fibrosis patient chronically colonized with Burkholderia vietnamiensis.
Fifteen serial Burkholderia cepacia complex isolates recovered over a period of 4 years from a single cystic fibrosis patient were analysed for genomovar status by means of recA sequence determination, and genetic relatedness by RAPD-PCR. Twelve isolates were assigned as Burkholderia vietnamiensis, two as Burkholderia cenocepacia and one as Burkholderia multivorans. B. vietnamiensis persisted in the airways during 4 years, except in three occasions when B. cenocepacia or B. multivorans were isolated. The patient was chronically colonized by B. vietnamiensis with the RAPD-profile 12 and transiently by the RAPD-profile 15.
Carvalho
Grasiella M V
GM
Disciplina de Microbiologia e Imunologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87/3 degrees andar, Vila Isabel, RJ, 20551-030, Brazil.
Carvalho
Ana Paula D'A
AP
Folescu
Tânia W
TW
Higa
Laurinda
L
Teixeira
Lucia M
LM
Plotkowski
Maria C M
MC
Merquior
Vânia L
VL
Albano
Rodolpho M
RM
Marques
Elizabeth A
EA
D000293:Adolescent; D001938:Brazil; D019117:Burkholderia / Q000302:isolation & purification*; D042602:Burkholderia cepacia complex / Q000302:isolation & purification; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D008297:Male