PET imaging and optical imaging with D-luciferin [11C]methyl ester and D-luciferin [11C]methyl ether of luciferase gene expression in tumor xenografts of living mice.
New carbon-11 labeled D-luciferin analogs D-luciferin [(11)C]methyl ester ([(11)C]LMEster, [(11)C]1) and D-luciferin [(11)C]methyl ether ([(11)C]LMEther, [(11)C]2) were synthesized in 25-55% radiochemical yield. PET studies with [(11)C]LMEster and [(11)C]LMEther demonstrate a lower retention of the C-11 label at 45 min post-injection in luciferase expression tumor. Optical imaging with unlabeled substrate D-luciferin and radiotracers [(11)C]LMEster and [(11)C]LMEther gave tumor luciferase images within a few minutes of photon counting.
Wang
Ji-Quan
JQ
Department of Radiology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Pollok
Karen E
KE
Cai
Shanbao
S
Stantz
Keith M
KM
Hutchins
Gary D
GD
Zheng
Qi-Huang
QH
D000818:Animals; D002247:Carbon Isotopes; D004952:Esters / Q000737:chemistry; D049454:Firefly Luciferin / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic; D008156:Luciferases / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D008738:Methyl Ethers / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000235:genetics / Q000378:metabolism; D055095:Optics and Photonics; D049268:Positron-Emission Tomography / Q000379:methods*; D013997:Time Factors; D023041:Xenograft Model Antitumor Assays
D002247:Carbon Isotopes; D004952:Esters; D008738:Methyl Ethers; D049454:Firefly Luciferin; D008156:Luciferases