title: Association between pemphigus vulgaris and human leukocyte antigen in Han nation of northeast China.
abstract: OBJECTIVE
  To investigate the relationship between pemphigus vulgaris (PV) and human leukocyte antigen (HLA) in Han nation of northeast China.
  
  
  METHODS
  Standard microcytotoxicity test and polymerase chain reaction-sequence specific primers method were used to detect the HLA class I antigens and HLA-DRB1 and DQB1 alleles in 27 patients with PV and results were compared with control group.
  
  
  RESULTS
  Gene and phenotype frequencies of HLA-A3, A26(10), B60(40), and B13 (27.99%, 48%; 16.11%, 30%; 23.02%, 41%; 16.11%, 30%, respectively) increased significantly in PV group compared with control (1.01%, 2%; 0.5%, 1%; 4.61%, 9%; 5.13%, 10%, respectively). After P value correction, the difference of A3, A26 (10), and B60 (40) between the two groups was still significant. The gene frequencies of HLA-DRB1*140x (1401, 1404, 1405, 1407, 1408), DRB1*120x, and DQB1*0503 alleles in PV group (42.26%, 25.46%, and 23.02%) were significantly higher than control group (5.09%, 7.74%, and 1.89%). After P value correction, the difference was still significant between the two groups.
  
  
  CONCLUSIONS
  PV significantly relates with HLA in PV patients of Han nation of northeast China.
authors.affiliation: Department of Dermatology, First Affiliated Hospital, China Medical University, Shenyang 110001. gength@yahoo.com.cn
authors.forename: Long
authors.initials: L
authors.lastname: Geng
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Zhai
authors.forename: Ya-Ni
authors.initials: YN
authors.lastname: Lu
authors.forename: Fang-Ji
authors.initials: FJ
authors.lastname: Song
authors.forename: Hong-Duo
authors.initials: HD
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000208:ethnology; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D015234:HLA-A Antigens / Q000235:genetics*; D015791:HLA-A3 Antigen / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D008875:Middle Aged; D010392:Pemphigus / Q000235:genetics*; D010641:Phenotype
chemical_list: D015234:HLA-A Antigens; D015791:HLA-A3 Antigen; D015235:HLA-B Antigens; C081918:HLA-B60 antigen; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains