title: Functional divergence of duplicated c-myc genes in a tetraploid fish, the common carp (Cyprinus carpio).
abstract: The proto-oncogene c-myc is thought to be one of the most important genes in controlling cell proliferation. In a tetraploid fish, two c-myc genes (CAM1 and CAM2) were previously isolated from the common carp, Cyprinus carpio, and were shown to have different expression patterns in adult tissues. Here we found that CAM1 and CAM2 proteins had distinct properties in terms of their transcription regulation system, formation of the transcription activator complex Myc/Max, and transcriptional activation of the target gene. These results showed that the two carp c-Myc proteins have overlapping but distinct functions, suggesting that CAM1 and CAM2 are evolving to acquire different functions after an earlier tetraploidization event.
authors.affiliation: Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Konan 4, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Futami
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Okamoto
mesh_terms: D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D020440:Gene Duplication; D005786:Gene Expression Regulation; D016259:Genes, myc; D011123:Polyploidy; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger