Interaction of mouse Pem protein and cell division cycle 37 homolog.
Mouse Pem, a homeobox gene, encodes a protein consisting of 210 amino acid residues. To study the function of mouse Pem protein, we used the yeast two-hybrid system to screen the library of 7-day mouse embryo with full-length mouse Pem cDNA. Fifty-two colonies were obtained after 1.57 x 10(8) colonies were screened by nutrition limitation and beta-galactosidase assay. Seven individual insert fragments were obtained from the library, and three of them were identified, one of which was confirmed to be the cell division cycle 37 (Cdc37) homolog gene by sequencing. The interaction between mouse Pem and Cdc37 homolog was then confirmed by glutathione S-transferase pull-down assay, and the possible interaction model was suggested.
Guo
Fen
F
College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Li
Yue-Qin
YQ
Li
Shi-Qian
SQ
Luo
Zhi-Wen
ZW
Zhang
Xin
X
Tang
Dong-Sheng
DS
Zhou
Tian-Hong
TH
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D018832:Molecular Chaperones / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D018797:Cell Cycle Proteins; D018398:Homeodomain Proteins; D018832:Molecular Chaperones; D014157:Transcription Factors; C513271:Cdc37 protein, mouse; C498048:Rhox5 protein, mouse