Cardiac sarcolemma of the hamster. Enrichment of the (Na+ + K+)-ATPase.
Cardiac sarcolemma was prepared from normal hamsters using gentle homogenization, extraction with 0.6 M KCl and continuous density gradient centrifugation. The final fraction exhibited high (Na+ + K+)-ATPase activity (24 micromol/mg per h) and contained minimal contamination from mitochondria, myofibrils and lysosomes.
Pang
D C
DC
Department of Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond, Va 23298, USA.
Weglicki
W B
WB
D000818:Animals; D001671:Biochemistry / Q000379:methods; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D008247:Lysosomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism; D011189:Potassium Chloride / Q000737:chemistry; D012508:Sarcolemma / Q000737:chemistry*; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry*; D013395:Sucrose / Q000494:pharmacology
D013395:Sucrose; D011189:Potassium Chloride; D000254:Sodium-Potassium-Exchanging ATPase