title: A novel autosomal recessive non-syndromic deafness locus, DFNB66, maps to chromosome 6p21.2-22.3 in a large Tunisian consanguineous family.
abstract: Hereditary non-syndromic deafness is extremely heterogeneous. Autosomal recessive forms account for approximately 80% of genetic cases. Autosomal recessive non-syndromic sensorineural deafness segregating in a large consanguineous Tunisian family was mapped to chromosome 6p21.2-22.3. A maximum lod score of 5.36 at theta=0 was obtained for the polymorphic microsatellite marker IR2/IR4. Haplotype analysis defined a 16.5-Mb critical region between microsatellite markers D6S1602 and D6S1665. The screening of 3 candidate genes, COL11A2, BAK1 and TMHS, did not reveal any disease causing mutation, suggesting that this is a novel deafness locus, which has been named DFNB66. A search in the Human Cochlear EST Library for ESTs located in this critical interval allowed us to identify several candidates. Further investigations on these candidates are needed in order to identify the deafness-causing gene in this Tunisian family.
authors.affiliation: Laboratoire de Génétique Moléculaire Humaine, Faculté de Médecine de Sfax, Tunisie.
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Tlili
authors.forename: Minna
authors.initials: M
authors.lastname: Männikkö
authors.forename: Ilhem
authors.initials: I
authors.lastname: Charfedine
authors.forename: Imed
authors.initials: I
authors.lastname: Lahmar
authors.forename: Zeineb
authors.initials: Z
authors.lastname: Benzina
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ben Amor
authors.forename: Nabil
authors.initials: N
authors.lastname: Driss
authors.forename: Leena
authors.initials: L
authors.lastname: Ala-Kokko
authors.forename: Mohamed
authors.initials: M
authors.lastname: Drira
authors.forename: Saber
authors.initials: S
authors.lastname: Masmoudi
authors.forename: Hammadi
authors.initials: H
authors.lastname: Ayadi
mesh_terms: D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D004252:DNA Mutational Analysis; D017931:DNA Primers; D003638:Deafness / Q000235:genetics*; D020224:Expressed Sequence Tags; D005808:Genes, Recessive / Q000235:genetics; D006801:Humans; D008126:Lod Score; D018895:Microsatellite Repeats / Q000235:genetics; D010375:Pedigree; D017422:Sequence Analysis, DNA; D014416:Tunisia
chemical_list: D017931:DNA Primers