Imaging the cAMP-dependent signal transduction pathway.
In recent years, the development of new technologies based on the green fluorescent protein and FRET (fluorescence resonance energy transfer) has introduced a new perspective in the study of cAMP signalling. Real-time imaging of fluorescent biosensors is making it possible to visualize cAMP dynamics directly as they happen in intact, living cells, providing important and original insights for our understanding of the spatiotemporal organization of the cAMP/PKA (protein kinase A) signalling pathway.
Zaccolo
M
M
Dulbecco Telethon Institute at the Venetian Institute of Molecular Medicine, Via Orus 2, 35100 Padova, Italy. manuela.zaccolo@unipd.it
Cesetti
T
T
Di Benedetto
G
G
Mongillo
M
M
Lissandron
V
V
Terrin
A
A
Zamparo
I
I
D000818:Animals; D015374:Biosensing Techniques; D002451:Cell Compartmentation; D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D015290:Second Messenger Systems / Q000502:physiology*
D049452:Green Fluorescent Proteins; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases