title: Signals of seminal vesicle autoantigen suppresses bovine serum albumin-induced capacitation in mouse sperm.
abstract: Capacitation is the prerequisite process for sperm to gain the ability for successful fertilization. Unregulated capacitation will cause sperm to undergo a spontaneous acrosome reaction and then fail to fertilize an egg. Seminal plasma is thought to have the ability to suppress sperm capacitation. However, the mechanisms by which seminal proteins suppress capacitation have not been well understood. Recently, we demonstrated that a major seminal vesicle secretory protein, seminal vesicle autoantigen (SVA), is able to suppress bovine serum albumin (BSA)-induced mouse sperm capacitation. To further identify the mechanism of SVA action, we determine the molecular events associated with SVA suppression of BSA's activity. In this communication, we demonstrate that SVA suppresses the BSA-induced increase of intracellular calcium concentration ([Ca2+]i), intracellular pH (pH(i)), the cAMP level, PKA activity, protein tyrosine phosphorylation, and capacitation in mouse sperm. Besides, we also found that the suppression ability of SVA against BSA-induced protein tyrosine phosphorylation and capacitation could be reversed by dbcAMP (a cAMP agonist).
authors.affiliation: Department of Biochemistry and Graduate Institute of Medical Sciences, School of Medicine, Taipei Medical University, Taipei, Taiwan. rita1204@tmu.edu.tw
authors.forename: Yen Hua
authors.initials: YH
authors.lastname: Huang
authors.forename: Shin Peih
authors.initials: SP
authors.lastname: Kuo
authors.forename: Mei Hsiang
authors.initials: MH
authors.lastname: Lin
authors.forename: Chwen Ming
authors.initials: CM
authors.lastname: Shih
authors.forename: Sin Tak
authors.initials: ST
authors.lastname: Chu
authors.forename: Chih Chun
authors.initials: CC
authors.lastname: Wei
authors.forename: Tasi Jung
authors.initials: TJ
authors.lastname: Wu
authors.forename: Yee Hsiung
authors.initials: YH
authors.lastname: Chen
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000378:metabolism*; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002413:Cations, Divalent / Q000737:chemistry; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D019000:Phosphotyrosine / Q000378:metabolism; D029607:Seminal Vesicle Secretory Proteins / Q000378:metabolism*; D012709:Serum Albumin / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D013075:Sperm Capacitation / Q000187:drug effects* / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology
chemical_list: D001324:Autoantigens; D002413:Cations, Divalent; D029607:Seminal Vesicle Secretory Proteins; D012709:Serum Albumin; C085073:seminal vesicle autoantigen; D019000:Phosphotyrosine; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D002118:Calcium