Synthesis of a new pair of fluorescence resonance energy transfer donor and acceptor dyes and its use in a protease assay.
A new, efficient and very robust fluorescence resonance energy transfer (FRET) system, which can be measured in a normal as well as in a time-resolved mode, was developed and its feasibility demonstrated in a protease assay format.
Kainmüller
Eva Katharina
EK
Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstr. 21, D-79104 Freiburg, Germany.
Ollé
Eulàlia Pinyol
EP
Bannwarth
Willi
W
D002384:Catalysis; D004952:Esters / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006571:Heterocyclic Compounds / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D010447:Peptide Hydrolases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D004952:Esters; D005456:Fluorescent Dyes; D006571:Heterocyclic Compounds; D010447:Peptide Hydrolases