A novel, disposable, screen-printed amperometric biosensor for glucose in serum fabricated using a water-based carbon ink.
Screen-printed amperometric glucose biosensors have been fabricated using a water-based carbon ink. The enzyme glucose oxidase (GOD) and the electro-catalyst cobalt phthalocyanine were mixed with the carbon ink prior to the screen-printing process; therefore, biosensors are prepared in a one-step fabrication procedure. Optimisation of the biosensor performance was achieved by studying the effects of pH, buffer strength, and applied potential on the analytical response. Calibration studies were performed under optimum conditions, using amperometry in stirred solution, with an operating potential of +500 mV versus SCE. The sensitivity was found to be 1170 nA mM(-1), with a linear range of 0.025-2 mM; the former represents the detection limit. The disposable amperometric biosensor was evaluated by carrying out replicate determinations on a sample of bovine serum. This was achieved by the method of multiple standard additions and included a correction for background currents arising from oxidizable serum components. The mean serum concentration was calculated to be 8.63 mM and compared well with the supplier's value of 8.3 mM; the coefficient of variation was calculated to be 3.3% (n=6).
Crouch
Eric
E
Centre for Research in Analytical, Materials, and Sensor Science, Faculty of Applied Sciences, University of the West of England, Bristol BS16 1QY, UK.
Cowell
David C
DC
Hoskins
Stephen
S
Pittson
Robin W
RW
Hart
John P
JP
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation* / Q000379:methods; D002244:Carbon / Q000737:chemistry*; D004209:Disposable Equipment; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005949:Glucose Oxidase / Q000737:chemistry*; D007281:Ink; D011327:Printing / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry*
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D014867:Water; D002244:Carbon; D005949:Glucose Oxidase