title: [Diminished apoptosis in nasal mucosa of patients with persistent allergic rhinitis].
abstract: OBJECTIVE
  To assess apoptosis frequency in the nasal mucosa of patients with perennial allergic rhinitis.
  
  
  METHODS
  Eleven patients and five healthy subjects accepted to participate, from 10 to 16 years old. None of them had evidence of acute infectious disease, tobacco exposure or use of corticosteroids, antileukotrienes, antihistamines or immunotherapy. After a clinical evaluation and a skin prick testing, a nasal symptoms questionnaire was applied and a nasal washing was performed. Two hundred cells were examined (Giemsa and eosin yellowish) and apoptosis was identified by immunohistochemistry (Active-Caspase-3) (p20L18, sc-1225. Santa Cruz Biothecnology, Inc. Santa Cruz).
  
  
  RESULTS
  The most common allergen was Dermatophagoides sp in 10 patients (91%). The total score of the nasal symptom questionnaire was 3 to 8 (median 6) in patients and 1 to 4 (median 2) in healthy subjects (p < 0.05, Mann Whitney U test). Frequency of eosinophils was 11 to 80% in patients and 0 to 1% in healthy subjects (p < 0.05, Z for proportions). Apoptosis was less frequent in patients (0 to 5, median 2) than in healthy subjects (4 to 16, median 8) (p < 0.01, Mann Whitney U test).
  
  
  CONCLUSIONS
  Perennial allergic rhinitis may be associated with decreased apoptosis in the nasal mucosa.
authors.affiliation: Servicio de otorrinolaringología, Centro Medico Nacional La Raza, IMSS, Mexico.
authors.forename: Vivián
authors.initials: V
authors.lastname: Pastrana González
authors.forename: Bertha Beatriz
authors.initials: BB
authors.lastname: Montaño Velázquez
authors.forename: Sara
authors.initials: S
authors.lastname: Huerta Yépez
authors.forename: Vilma Carolina
authors.initials: VC
authors.lastname: Bekker Méndez
authors.forename: Ramón
authors.initials: R
authors.lastname: Campillo Navarrete
authors.forename: María del Refugio
authors.initials: Mdel R
authors.lastname: Cisneros Salazar
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Mogica Martínez
authors.forename: Roberto
authors.initials: R
authors.lastname: Salazar Santiago
authors.forename: Martín
authors.initials: M
authors.lastname: Becerril Angeles
authors.forename: Kathrine
authors.initials: K
authors.lastname: Jáuregui Renaud
mesh_terms: D000328:Adult; D000485:Allergens / Q000009:adverse effects; D000818:Animals; D017209:Apoptosis; D003430:Cross-Sectional Studies; D040002:Dermatophagoides pteronyssinus / Q000276:immunology; D004802:Eosinophilia / Q000209:etiology / Q000473:pathology; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D009297:Nasal Mucosa / Q000473:pathology*; D012221:Rhinitis, Allergic, Perennial / Q000473:pathology*; D012720:Severity of Illness Index; D012882:Skin Tests; D011795:Surveys and Questionnaires; D007507:Therapeutic Irrigation
chemical_list: D000485:Allergens