title: A direct role for dual oxidase in Drosophila gut immunity.
abstract: Because the mucosal epithelia are in constant contact with large numbers of microorganisms, these surfaces must be armed with efficient microbial control systems. Here, we show that the Drosophila nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme, dual oxidase (dDuox), is indispensable for gut antimicrobial activities. Adult flies in which dDuox expression is silenced showed a marked increase in mortality rate even after a minor infection through ingestion of microbe-contaminated food. This could be restored by the specific reintroduction of dDuox, demonstrating that this oxidase generates a unique epithelial oxidative burst that limits microbial proliferation in the gut. Thus, oxidant-mediated antimicrobial responses are not restricted to the phagocytes, but rather are used more broadly, including in mucosal barrier epithelia.
authors.affiliation: Division of Molecular Life Science and Center for Cell Signaling Research, Ewha Woman's University, Seoul 120-750, South Korea.
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Ha
authors.forename: Chun-Taek
authors.initials: CT
authors.lastname: Oh
authors.forename: Yun Soo
authors.initials: YS
authors.lastname: Bae
authors.forename: Won-Jae
authors.initials: WJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001419:Bacteria / Q000254:growth & development* / Q000276:immunology; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D015169:Colony Count, Microbial; D004064:Digestive System / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D004330:Drosophila / Q000201:enzymology* / Q000276:immunology* / Q000382:microbiology; D007113:Immunity, Innate; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000276:immunology; D013481:Superoxides / Q000378:metabolism
chemical_list: D002712:Chlorides; D017382:Reactive Oxygen Species; C505384:dual oxidase, Drosophila; D013481:Superoxides; D002374:Catalase; D019255:NADPH Oxidases