title: Synthesis and antitumor activity of 5-bromo-1-mesyluracil.
abstract: Large-scale preparation of 5-bromo-1-mesyluracil (BMsU) 4 has been optimized. BMsU was synthesized by condensation of silylated 5-bromouracil and MsCl in acetonitrile or by the reaction of 5-bromouracil with MsCl in pyridine. The same product was obtained by bromination of 1-mesyluracil. The purpose of this study was to elucidate the effects of BMsU on the biosynthetic activity of tumor cell enzymes involved in DNA, RNA and protein syntheses, and in de novo and salvage pyrimidine and purine syntheses. Investigations were performed in vitro on human cervix carcinoma cells (HeLa). BMsU displayed inhibitory effects on DNA and RNA syntheses in HeLa cells after 24 h of treatment. De nova biosynthesis of pyrimidine and purine was also affected. Antitumor activity of BMsU is closely associated with its inhibitory activity on the enzymes that play an important role in the metabolism of tumor cells. In vivo antitumor activity of BMsU was also investigated. The model used in investigations was a mouse anaplastic mammary carcinoma transplanted into the thigh of the right leg of CBA mice. Significant reduction in tumor growth time was achieved with BmsU administered at a dose of 50 mg/kg.
authors.affiliation: Clinical Hospital Osijek, Osijek, Croatia.
authors.forename: Ljubica
authors.initials: L
authors.lastname: Glava-Obrovac
authors.forename: Ivan
authors.initials: I
authors.lastname: Karner
authors.forename: Marina
authors.initials: M
authors.lastname: Pavlak
authors.forename: Marko
authors.initials: M
authors.lastname: Radacić
authors.forename: Jelena
authors.initials: J
authors.lastname: Kasnar-Samprec
authors.forename: Biserka
authors.initials: B
authors.lastname: Zinić
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000737:chemistry; D005260:Female; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008956:Models, Chemical; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000188:drug therapy*; D011687:Purines / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D013450:Sulfones / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors; D014498:Uracil / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C506204:5-bromo-1-mesyluracil; D000970:Antineoplastic Agents; D011687:Purines; D011743:Pyrimidines; D013450:Sulfones; D014498:Uracil; D012313:RNA; D004247:DNA