Fast and versatile multiresidue method for the analysis of botanical insecticides on fruits and vegetables by HPLC/DAD/MS.
A simple multiresidue method for screening analysis of 12 botanical insecticides used by organic farmers has been developed. The method involves a rapid and small-scale extraction procedure with acetonitrile. For all fruit and vegetable samples, there was no need for clean up. Rotenone, azadirachtin, ryanodines, and pyrethrins can be separated by high-performance liquid chromatography, quantified, and confirmed with a diode array detector (DAD) and atmospheric pressure chemical ionization mass spectrometry (APCI-MS) in the select ion-monitoring mode (SIM). The majority of pesticide recoveries for various fruits and vegetables were >70% in the concentration range from 0.01 to 5 mg/kg. The limit of quantitation for most of the pesticides was 0.01 mg/kg, with the majority of relative standard deviations (RSD) mostly below 10%.
Caboni
Pierluigi
P
Dipartimento di Tossicologia, Università di Cagliari, via Ospedale 72, 09124 Cagliari, Italy. caboni@unica.it
Sarais
Giorgia
G
Angioni
Alberto
A
Garau
Vincenzo Luigi
VL
Cabras
Paolo
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D007306:Insecticides / Q000032:analysis*; D036701:Limonins / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D011722:Pyrethrins / Q000032:analysis; D012402:Rotenone / Q000031:analogs & derivatives / Q000032:analysis; D012433:Ryanodine / Q000032:analysis; D014675:Vegetables / Q000737:chemistry*
D007306:Insecticides; D036701:Limonins; D011722:Pyrethrins; D012402:Rotenone; D012433:Ryanodine; C107676:deguelin; C010329:azadirachtin