title: [Analysis of secreted proteases of Trichophyton rubrum].
abstract: Secreted proteases are thought as potential virulent factors of Tricophyton rubrum. Based on cDNA libraries of 6 physiological phases of Tricophyton rubrum, 9683 unique ESTs were obtained by DNA sequencing. By bioinformatic analysis, 18 ESTs of putative secreted proteases were obtained from the established ESTs library, including 4 secreted peptidases, 1 secreted metalloprotease, 2 extracellular serine proteases, 1 secreted aspartic proteinase, 9 secreted subtilisin-like proteases and 1 vacuolar serine protease. These secreted proteases are related to nutrient uptake, lesion extension and host immune responses elicitation in the process of T. rubrum infection. These results provide a clue to further research on the infection and pathogencity of T. rubrum.
authors.affiliation: State Key Laboratory for Molecular Virology and Genetic Engineering, Chinese Center for Disease Control and Prevention, Beijing 100176, China. lengwenchuan@hotmail.com
authors.forename: Wen-Chuan
authors.initials: WC
authors.lastname: Leng
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Wang
authors.forename: Can-Dong
authors.initials: CD
authors.lastname: Wei
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
mesh_terms: D000595:Amino Acid Sequence; D020224:Expressed Sequence Tags; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D014249:Trichophyton / Q000201:enzymology* / Q000472:pathogenicity
chemical_list: D010447:Peptide Hydrolases