Chromatographic separation of (-)-ephedrine and (+)-pseudoephedrine in the traditional Chinese medicinal preparation Jiketing granule.
(-)-Ephedrine (EP) and (+)-Pseudoephedrine (PEP) in Jiketing granule were separated by TLC, RP-HPLC, GC, HPLC-MS, and the chromatographic phenomenon was interpreted by the topological indices. The observed chromatographic separations of investigated components were compared. This comparison indicated that simply methods as C18 RP-HPLC and GC are the more effective techniques, and more suitable than others for this preparation, for the separation of two alkaloids.
Luhua
Zhao
Z
Analytic Center, China Pharmaceutical University, Nanjing, China.
Fang
Yan
Y
Lili
Yang
Y
Bingren
Xiang
X
D000697:Central Nervous System Stimulants / Q000302:isolation & purification*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004809:Ephedrine / Q000302:isolation & purification*; D013058:Mass Spectrometry; D012044:Regression Analysis; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000697:Central Nervous System Stimulants; D004365:Drugs, Chinese Herbal; D004809:Ephedrine