[Establishment of a rhesus haploidentical hematopoietic stem cell and mesenchymal stem cell transplantation model by nonmyeloablative conditioning].
OBJECTIVE
To establish rhesus haploidentical hematopoietic stem cell transplantation model by nonmyeloablative conditioning, and examine the effects of mesenchymal stem cells (MSC) in haploidentical transplantation.


METHODS
The recipient haploidentical rhesus monkeys were conditioned with a nonmyeloablative regimen consisted of fludarabine, cyclophosphamide, 200 cGy total body irradiation, and rabbit anti-human thymocyte globulin. Cyclosporine A, mycophenolate mofetil and anti CD25 antibody were used for graft versus host disease (GVHD) prophylaxis. Rhesus monkeys in one group were given hematopoietic stem cell (HSC) only, while in the other group HSC combined with MSC. The differences in hematopoiesis recovery, chimerism level, and GVHD between the two groups were evaluated.


RESULTS
Stable chimerism could be achieved in recipient monkeys. Hematopoiesis recovery was mainly related with chimerism level. MSC seemed capable of facilitating HSC engraftment, as there were more mixed chimerism and less GVHD occurrence in the HSC combined with MSC recipient group.


CONCLUSIONS
A rhesus haploidentical hematopoietic stem cell transplantation model is successfully established by nonmyeloablative conditioning. MSC was of great benefit to haploidentical transplantation.
Liu
Li-hui
LH
Department of Hematology, Affiliated Hospital of Academy of Military Medicine Science, Beijing 100039, China.
Sun
Qi-yun
QY
Hu
Kai-xun
KX
Huang
Ya-jing
YJ
Fan
Chuan-bo
CB
Sun
Zhao
Z
Yao
Bo
B
Guo
Mei
M
Zhao
Chun-hua
CH
Ai
Hui-sheng
HS
D000818:Animals; D046528:Chimerism; D006086:Graft vs Host Disease / Q000517:prevention & control; D006238:Haploidy; D018380:Hematopoietic Stem Cell Transplantation; D008253:Macaca mulatta / Q000235:genetics* / Q000601:surgery; D045164:Mesenchymal Stem Cell Transplantation; D023421:Models, Animal; D019172:Transplantation Conditioning