title: Batch and fed-batch production of butyric acid by clostridium butyricum ZJUCB.
abstract: The production of butyric acid by Clostridium butyricum ZJUCB at various pH values was investigated. In order to study the effect of pH on cell growth, butyric acid biosynthesis and reducing sugar consumption, different cultivation pH values ranging from 6.0 to 7.5 were evaluated in 5-L bioreactor. In controlled pH batch fermentation, the optimum pH for cell growth and butyric acid production was 6.5 with a cell yield of 3.65 g/L and butyric acid yield of 12.25 g/L. Based on these results, this study then compared batch and fed-batch fermentation of butyric acid production at pH 6.5. Maximum value (16.74 g/L) of butyric acid concentration was obtained in fed-batch fermentation compared to 12.25 g/L in batch fermentation. It was concluded that cultivation under fed-batch fermentation mode could enhance butyric acid production significantly (P<0.01) by C. butyricum ZJUCB.
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China. gqhe@zju.edu.cn
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: He
authors.forename: Qing
authors.initials: Q
authors.lastname: Kong
authors.forename: Qi-he
authors.initials: QH
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Ruan
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D020148:Butyric Acid / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D046972:Clostridium butyricum / Q000254:growth & development* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration
chemical_list: D020148:Butyric Acid; D005947:Glucose