Identification of genes differentially expressed in wild type and Purkinje cell degeneration mice.
Purkinje cell degeneration (pcd) mice are characterized by death of virtually all cerebellar Purkinje cells by postnatal day 30. In this study, we used DNA microarray analysis to investigate differences in gene expression between the brains of wild type and pcd mice on postnatal day 20, before the appearance of clear-cut phenotypic abnormalities. We identified 300 differentially expressed genes, most of which were involved in metabolic and physiological processes. Among the differentially expressed genes were several calcium binding proteins including calbindin-28k, paravalbumin, matrix gamma-carboxyglutamate protein and synaptotagamins 1 and 13, suggesting the involvement of abnormal Ca2+ signaling in the pcd phenotype.
Xiao
Rui
R
Department of Animal Biotechnology; Bio-Organ Research Center/Institute of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Korea.
Park
Youngsook
Y
Dirisala
Vijaya R
VR
Zhang
Ya-Ping
YP
Um
Sang June
SJ
Lee
Hoon Taek
HT
Park
Chankyu
C
D000483:Alleles; D000818:Animals; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D051379:Mice; D009410:Nerve Degeneration / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011689:Purkinje Cells / Q000378:metabolism / Q000473:pathology* / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005819:Genetic Markers