title: Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11.
abstract: Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with an scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01.
authors.affiliation: Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
authors.forename: Man
authors.initials: M
authors.lastname: Cheng
authors.forename: Ceci W L
authors.initials: CW
authors.lastname: Chan
authors.forename: Randy C F
authors.initials: RC
authors.lastname: Cheung
authors.forename: Rama Kamesh
authors.initials: RK
authors.lastname: Bikkavilli
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.forename: Shannon W N
authors.initials: SW
authors.lastname: Au
authors.forename: Paul K S
authors.initials: PK
authors.lastname: Chan
authors.forename: Susanna S T
authors.initials: SS
authors.lastname: Lee
authors.forename: Gregory
authors.initials: G
authors.lastname: Cheng
authors.forename: Walter K K
authors.initials: WK
authors.lastname: Ho
authors.forename: Wing-Tai
authors.initials: WT
authors.lastname: Cheung
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D003429:Cross Reactions / Q000276:immunology; D017370:Interleukin-11 / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000276:immunology*; D051381:Rats; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry / Q000276:immunology*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000914:Antibodies, Viral; D017370:Interleukin-11; D019590:Nucleocapsid Proteins