Analysis of hedgehog interacting protein in the brain and its expression in nitric oxide synthase-positive cells.
Hedgehog interacting protein (Hip) and Patched 1 (Ptc1) regulate the cell responses to the morphogen Sonic Hedgehog (Shh). Here, we compare the relative expression patterns of Shh, Hip and Ptc1 transcripts in the E13.5 mouse brain embryo. We observe that the expression of Hip and Ptc1 often overlaps and is found close to Shh-expressing cells, suggesting that both proteins are required for controlling Shh signals. In the adult striatum in which Ptc1 is not detected, we show that a majority of Hip-expressing cells correspond to neurons expressing the neuronal form of nitric oxide synthase. These data raise the hypothesis for a functional link between nitric oxide and Shh signaling and for a nonredundant role of Hip and Ptc1 in the adult brain.
Loulier
Karine
K
CNRS, Signal Transduction and Developmental Neuropharmacology, Laboratory of Cellular and Molecular Neurobiology, Alfred Fessard Institute of Neurobiology, Gif sur Yvette, France.
Ruat
Martial
M
Traiffort
Elisabeth
E
D000367:Age Factors; D000818:Animals; D016375:Antisense Elements (Genetics); D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003342:Corpus Striatum / Q000196:embryology* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000235:genetics / Q000378:metabolism*; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
D016375:Antisense Elements (Genetics); D002352:Carrier Proteins; D053823:Hedgehog Proteins; C498447:Hhip protein, mouse; D008562:Membrane Glycoproteins; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; C000606240:Ptch1 protein, mouse; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D015534:Trans-Activators; D009569:Nitric Oxide; D052248:Nitric Oxide Synthase Type I; C496321:Nos1 protein, mouse