Roles of eukaryotic ribosomal proteins in maturation and transport of pre-18S rRNA and ribosome function.
Despite the rising knowledge about ribosome function and structure and how ribosomal subunits assemble in vitro in bacteria, the in vivo role of many ribosomal proteins remains obscure both in pro- and eukaryotes. Our systematic analysis of yeast ribosomal proteins (r-proteins) of the small subunit revealed that most eukaryotic r-proteins fulfill different roles in ribosome biogenesis, making them indispensable for growth. Different r-proteins control distinct steps of nuclear and cytoplasmic pre-18S rRNA processing and, thus, ensure that only properly assembled ribosomes become engaged in translation. Comparative analysis of dynamic and steady-state maturation assays revealed that several r-proteins are required for efficient nuclear export of pre-18S rRNA, suggesting that they form an interaction platform with the export machinery. In contrast, the presence of other r-proteins is mainly required before nuclear export is initiated. Our studies draw a correlation between the in vitro assembly, structural localization, and in vivo function of r-proteins.
Ferreira-Cerca
Sébastien
S
Institut für Biochemie, Genetik und Mikrobiologie, Universität Regensburg, Universitätsstr 31, 93053 Regensburg, Germany.
Pöll
Gisela
G
Gleizes
Pierre-Emmanuel
PE
Tschochner
Herbert
H
Milkereit
Philipp
P
D005057:Eukaryotic Cells / Q000378:metabolism*; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D012337:RNA, Ribosomal, 18S; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins