title: Validation and implementation of a liquid chromatography/tandem mass spectrometry assay to quantitate dimethyl benzoylphenylurea (BPU) and its five metabolites in human plasma and urine for clinical pharmacology studies.
abstract: A method has been developed for the quantitation of N-[4-(5-bromo-2-pyrimidinyloxy)-3-methylphenyl]-N'-(2-dimethylamino-benzoyl)urea (BPU) and its metabolites in human plasma and urine. BPU and metabolites were separated on a C18 column with acetonitrile-water mobile phase containing 0.1% formic acid using isocratic flow for 5 min. The analytes were monitored by tandem mass spectrometry. Calibration curves were generated over the range of 2.5-500 ng/mL for BPU, mmBPU, and aminoBPU in plasma; and 0.1-20, 0.1-20, 0.5-100, 10-2000, 1-200, and 3-600 ng/mL for BPU, mmBPU, aminoBPU, G280, G308, and G322 in urine, respectively. The method has been successfully applied to study the pharmacokinetics of BPU.
authors.affiliation: The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Bunting-Blaustein Cancer Research Building, Room 1M86, 1650 Orleans Street, Baltimore, MD 21231-1000, USA.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Rudek
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.forename: Yelena
authors.initials: Y
authors.lastname: Zabelina
authors.forename: Runyan
authors.initials: R
authors.lastname: Jin
authors.forename: Wells A
authors.initials: WA
authors.lastname: Messersmith
authors.forename: Antonio C
authors.initials: AC
authors.lastname: Wolff
authors.forename: Sharyn D
authors.initials: SD
authors.lastname: Baker
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008782:Methylurea Compounds / Q000097:blood* / Q000652:urine*; D010671:Phenylurea Compounds / Q000032:analysis / Q000097:blood / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D008782:Methylurea Compounds; D010671:Phenylurea Compounds; C496369:dimethyl benzoylphenyl urea