title: Domain architectural census of eukaryotic gene products containing O-protein phosphatases.
abstract: Intricate molecular signalling within cellular environment is manifested through phosphorylation of proteins. Regulation of the phosphorylation state is executed through complex networking among kinases and their biochemical antagonists, the protein phosphatases. Protein dephosphorylation in eukaryotic systems is largely performed through four structurally distinct Ser/Thr and Tyr O-protein phosphatase superfamilies. 555 O-protein phosphatases, belonging to the four distinct families, could be identified using sensitive sequence search techniques across five eukaryotic model organisms (yeast, fly, worm, mouse and humans). These phosphatases could be grouped into 49 subfamilies associated with distinct domain architecture and discrete biochemical function. Only five of the architectures are shared across the five eukaryotic genomes. Interestingly, the number of occurrence of tyrosine phosphatases is correlated to the complexity of the genome. Analysis of domain architectures suggests amenability of the tyrosine phosphatases to occur in complex architectures unlike Ser/Thr phosphatases. Domain duplication and shuffling is shown as the customary mechanism for the evolution of the phosphatases. Several architectures are common between humans and other genomes, which are probably non-linearly inherited in humans or specifically lost in several others.
authors.affiliation: National Centre for Biological Sciences, Tata Institute of Fundamental Research, UAS-GKVK Campus, Bangalore 560065, India.
authors.forename: Anirban
authors.initials: A
authors.lastname: Bhaduri
authors.forename: R
authors.initials: R
authors.lastname: Sowdhamini
mesh_terms: D000818:Animals; D019143:Evolution, Molecular; D016678:Genome; D006801:Humans; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational / Q000235:genetics*; D017434:Protein Structure, Tertiary / Q000235:genetics; D012568:Schizosaccharomyces; D020539:Sequence Analysis, Protein / Q000379:methods; D040681:Structural Homology, Protein
chemical_list: D010749:Phosphoprotein Phosphatases