Ultrastructural analysis of the chorda tympani nerve in chronic suppurative otitis media.
OBJECTIVE
To compare ultrastructure of the chorda tympani nerve by light and electron microscopy in patients with otosclerosis and chronic suppurative otitis media.


METHODS
Comparative prospective study.


METHODS
Tertiary care, referral medical center.


METHODS
The tympanic segments of chorda tympani nerves were collected for ultrastructural investigation in 20 cases with chronic suppurative otitis media and 10 cases with otosclerosis that underwent middle ear surgery.


RESULTS
Histopathologic examinations of the suppurative group showed that unmyelinated fibers were almost totally lost and replaced by collagen fibers. The thickness of the myelin sheaths was very slender, whereas some of them were atrophic. A substantial increase was encountered in the endoneural collagen substance and connective tissue, whereas inflammatory elements and edema were present occasionally. Degenerative alterations of the myelinated fibers mainly occurred in the form of adaxonal vacuoles. There was disorganization and separation of parallel lamellae of Schmidt-Lanterman clefts.


CONCLUSIONS
The chorda tympani nerve should be preserved in otologic surgery. However, inadvertent dissection of the chorda tympani nerve in chronic suppurative otitis media surgery will not possibly cause a postoperative disturbance in light of ultrastructural changes that occur in the nerve.
Uygur
Kemal
K
Gazi University Faculty of Medicine, ENT Department, Ankara, Turkey. uygur@gazi.edu.tr
Bayramoglu
Ismet
I
Nazikoğlu
Aysegul
A
Yilmaz
Metin
M
Bayazit
Yildirim
Y
Muftuoglu
Sevda
S
D001369:Axons / Q000473:pathology; D002814:Chorda Tympani Nerve / Q000473:pathology* / Q000601:surgery; D002908:Chronic Disease; D006801:Humans; D008854:Microscopy, Electron; D009410:Nerve Degeneration / Q000473:pathology; D009412:Nerve Fibers / Q000473:pathology; D009413:Nerve Fibers, Myelinated / Q000473:pathology; D010035:Otitis Media, Suppurative / Q000473:pathology* / Q000601:surgery; D010040:Otosclerosis / Q000473:pathology / Q000601:surgery; D011446:Prospective Studies; D012583:Schwann Cells / Q000473:pathology; D013201:Stapes Surgery