title: The ABP-120 C-end region from Entamoeba histolytica interacts with sulfatide, a new lipid target.
abstract: EhABP-120 is the first filamin identified in the parasitic protozoan Entamoeba histolytica. Filamins are a family of cross-linking actin-binding proteins that promote a dynamic orthogonal web. They have been reported to interact directly with more than 30 cellular proteins and some phosphoinositides. The biochemical consequences of these interactions may have either positive or negative effects on the cross-linking function and also form a link between the cytoskeleton and plasma membrane. In this study, the EhABP-120 carboxy-terminal domain (END) was biochemically characterized. This domain was able to associate to 3-sulfate galactosyl ceramide, a new lipid target for a member of the filamin family. Also, the END domain was able to dimerize "in vitro." Molecular modeling analysis showed that the dimeric region is stabilized by a disulfide bond. Electrostatic and docking studies suggest that an electropositive concave pocket at the dimeric END domain interacts simultaneously with several sulfogalactose moieties of the sulfatide.
authors.affiliation: Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Mexico, DF, Mexico.
authors.forename: Juan Daniel
authors.initials: JD
authors.lastname: Díaz-Valencia
authors.forename: Ma de Jesús
authors.initials: Mde J
authors.lastname: Almaraz-Barrera
authors.forename: Luis Enrique
authors.initials: LE
authors.lastname: Arias-Romero
authors.forename: Rosaura
authors.initials: R
authors.lastname: Hernandez-Rivas
authors.forename: Arturo
authors.initials: A
authors.lastname: Rojo-Domínguez
authors.forename: Nancy
authors.initials: N
authors.lastname: Guillén
authors.forename: Miguel
authors.initials: M
authors.lastname: Vargas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003285:Contractile Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000235:genetics / Q000378:metabolism*; D064448:Filamins; D008840:Microfilament Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013379:Substrate Specificity; D013433:Sulfoglycosphingolipids / Q000378:metabolism*; D014162:Transfection
chemical_list: D003285:Contractile Proteins; D064448:Filamins; D008840:Microfilament Proteins; D013433:Sulfoglycosphingolipids