Modified binary hammerhead ribozymes with high catalytic activity.
A series of binary hammerhead ribozymes was designed and assessed in terms of cleavage activity and nuclease resistance. Enhanced nuclease resistance of binary ribozymes was achieved by incorporation of Z-modified nucleotides at the selective positions along with addition of 3'-3-linked thymidine cap. These modified binary ribozymes efficiently cleave 190-nucleotides long MDR1 mRNA fragment and display catalytic activity much higher then respective full-length analogs.
Vorobjeva
M
M
Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
Gusseva
E
E
Repkova
M
M
Kovalev
N
N
Zenkova
M
M
Venyaminova
A
A
Vlassov
V
V
D001482:Base Composition; D001483:Base Sequence; D002384:Catalysis; D019450:Genes, MDR / Q000235:genetics; D005821:Genetic Techniques; D007700:Kinetics; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015202:Protein Engineering / Q000379:methods; D016337:RNA, Catalytic / Q000737:chemistry*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors
D016337:RNA, Catalytic; D012333:RNA, Messenger; C427204:hammerhead ribozyme