Diversity of "Candidatus Liberibacter asiaticus," based on the omp gene sequence.
Huanglongbing (yellow dragon disease) is a destructive disease of citrus. The etiological agent is a noncultured, phloem-restricted alpha-proteobacterium, "Candidatus Liberibacter africanus" in Africa and "Candidatus Liberibacter asiaticus" in Asia. In this study, we used an omp-based PCR-restriction fragment length polymorphism (RFLP) approach to analyze the genetic variability of "Ca. Liberibacter asiaticus" isolates. By using five different enzymes, each the 10 isolates tested could be associated with a specific combination of restriction profiles. The results indicate that the species "Ca. Liberibacter asiaticus," even within a given region, may comprise several different variants. Thus, omp-based PCR-RFLP analysis is a simple method for detecting and differentiating "Ca. Liberibacter asiaticus" isolates.
Bastianel
C
C
Laboratoire de Biologie Cellulaire et Moléculaire, UMR GDPP, INRA, Université Victor Segalen Bordeaux 2, 71 avenue Edouard Bourlaux BP81, 33883 Villenave D'Ornon Cedex, France.
Garnier-Semancik
M
M
Renaudin
J
J
Bové
J M
JM
Eveillard
S
S
D001208:Asia; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D015373:Bacterial Typing Techniques; D002957:Citrus / Q000382:microbiology*; D014644:Genetic Variation; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012230:Rhizobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
D001425:Bacterial Outer Membrane Proteins