title: Biomarker analysis in carcinoma ex pleomorphic adenoma at an early phase of carcinomatous transformation.
abstract: Diagnostic criteria for intracapsular carcinoma ex pleomorphic adenoma (CXPA) are subjective and vary among authors. Biomarker analysis, which could provide more objective evaluation of these tumors, has rarely been studied in intracapsular CXPA. Immunohistochemical evaluation of c-erbB-2, p53 protein, bcl-2, and Ki-67 was performed in 8 cases of CXPA at an early phase of malignant transformation (4 intracapsular and 4 minimally invasive) and in 17 pleomorphic adenomas (PA). In all cases of CXPA, p53 and Ki-67 were demonstrated predominantly in luminal cells of benign and malignant areas, significantly more in the latter. Few benign myoepithelial cells were p53 positive. c-erbB-2 reactivity was strongly associated with atypical luminal cells. Bcl-2 expression was weak and focal in malignant areas from 2 cases. In conclusion, both p53 and c-erbB-2 proteins appear to be involved at an early stage of malignization of PA. In PA with atypical cells, evaluation of the expression of these 2 markers provides more objective criteria for the diagnosis of intracapsular CXPA.
authors.affiliation: Department of Pathology, Medical Science Faculty, University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Leandro L L
authors.initials: LL
authors.lastname: Freitas
authors.forename: Vera C
authors.initials: VC
authors.lastname: Araújo
authors.forename: Marilia T
authors.initials: MT
authors.lastname: Martins
authors.forename: Carlos
authors.initials: C
authors.lastname: Chone
authors.forename: Agricio
authors.initials: A
authors.lastname: Crespo
authors.forename: Albina
authors.initials: A
authors.lastname: Altemani
mesh_terms: D008949:Adenoma, Pleomorphic / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002471:Cell Transformation, Neoplastic; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010307:Parotid Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis / Q000502:physiology; D013365:Submandibular Gland Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000502:physiology
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D018719:Erb-b2 Receptor Tyrosine Kinases; D016159:Tumor Suppressor Protein p53