[Detection of antigen-antibody interaction of human adenovirus by the method of surface plasmon resonance].
A possibility to detect adenoviral protein--hexon, using specific antibodies by surface plasmon resonance (SPR) was demonstrated. The hexon of the human adenovirus 2 (Ad2) binds to antibodies immobilized on the sensor surface treated by KNCS and protein A Staphylococcus aureus. The specificity of antihexon antibodies was demonstrated by indirect method of fluorescent antibodies (MFA) and cellular variant of the immunoassay (cELISA).
Nosach
L M
LM
Boltovets'
P M
PM
Povnytsia
O Iu
OIu
Zhovnovata
V L
VL
Zakharenko
O M
OM
Snopok
B A
BA
Shyrshov
Iu M
IuM
Diachenko
N S
NS
D000260:Adenoviruses, Human / Q000276:immunology / Q000302:isolation & purification*; D000914:Antibodies, Viral / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology*; D006801:Humans; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus