A silica-based monolithic column in capillary HPLC and CEC coupled with ESI-MS or electrospray-atmospheric-pressure laser ionization-MS.
We describe the successful coupling of CEC and capillary HPLC with the recently developed atmospheric-pressure laser ionization (APLI) method. APLI is suitable for selectively and sensitively ionizing nonpolar aromatic compounds at ambient pressure for subsequent mass-selective detection. The polycyclic aromatic hydrocarbons used as analytes are first separated either by CEC on a silica-based monolithic column or by capillary HPLC. The eluent, along with a sheath flow, is volatilized by microelectrospray and then selectively ionized by excimer laser (KrF*) radiation via two-photon excitation. A QTOF-MS is used as mass-selective detector. This interface combination makes soft ionization of thermally labile nonpolar aromatic analytes possible.
Droste
Stefan
S
Department of Analytical Chemistry, University of Wuppertal, Wuppertal, Germany.
Schellenträger
Marc
M
Constapel
Marc
M
Gäb
Siegmar
S
Lorenz
Matthias
M
Brockmann
Klaus J
KJ
Benter
Thorsten
T
Lubda
Dieter
D
Schmitz
Oliver J
OJ
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification*; D012822:Silicon Dioxide; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
D011084:Polycyclic Aromatic Hydrocarbons; D012822:Silicon Dioxide