Uptake of silica-coated nanoparticles by HeLa cells.
Nanoparticles have seen wide applications in cellular research and development. One major issue that is unclear is the uptake of nanoparticles by cells. In this study, we have investigated the uptake of silica-coated nanoparticles by HeLa cells, employing rhoadime 6G isothiocyanate (RITC)-doped nanoparticles as a synchronous fluorescent signal indicator. These nanoparticles were synthesized with reverse microemulsion. A few factors, such as nanoparticle concentration, incubation time and temperature, and serum and inhibitors in culture medium were assessed on the nanoparticle's cellular uptake. The experimental results demonstrated that uptake was maximum after a 6 h incubation and was higher at 37 degrees C than that at 4 degrees C. Nanoparticle uptake depended on the nanoparticle concentration and was inhibited by hyperosmolarity, K+ depletion. In addition, serum in culture medium decreased the cellular uptake of nanoparticles. The results indicated that the uptake of silica-coated nanoparticles by HeLa cells was a concentration-, time-, and energy-dependent endocytic process. Silica-coated nanoparticles could be transported into HeLa cells in part through adsorptive endocytosis and in part through fluid-phase endocytosis.
Xing
Xinli
X
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Biomedical Engineering Center, Hunan University, Changsha, China.
He
Xiaoxiao
X
Peng
Jiaofeng
J
Wang
Kemin
K
Tan
Weihong
W
D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000493:pharmacokinetics; D004705:Endocytosis / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D036103:Nanotechnology / Q000379:methods; D043942:Nanotubes / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry* / Q000493:pharmacokinetics; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D012822:Silicon Dioxide