title: [Cytokines secretion by peripheral blood mononuclear cells from hepatitis C patients after stimulation with synthetic peptides at the highly variable region].
abstract: OBJECTIVE
  Test the cytokines secretion by peripheral blood mononuclear cells (PBMC) from hepatitis C patients after stimulation with highly variable region (HVR1) synthetic peptides.
  
  
  METHODS
  ELISA was used to test the cytokines secretion, flow cytometry to group the proliferated cells, and the antigenicity of synthetic peptide was predicted by the computer modeling.
  
  
  RESULTS
  (1)There were PBMC proliferation when stimulated by HVR1 antigen synthetic peptides. (2) There was a rise of IFN-gamma, IL-4 and IL-10. But no rise of IL-2 and TNF-gamma was found. (3) The proliferated cells were mainly CD4+ lymphocytes.
  
  
  CONCLUSIONS
  PMBC from hepatitis C patients tend to secret Th2 cytokines after stimulation with HVR1 designed by the authors.
authors.affiliation: Department of Immunology, Institute of infectious diseases, The No. 302 Hospital of PLA, Beijing 100039, China.
authors.forename: Shu-ping
authors.initials: SP
authors.lastname: Chi
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
authors.forename: Hai-bin
authors.initials: HB
authors.lastname: Su
authors.forename: Bo-an
authors.initials: BA
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006526:Hepatitis C / Q000097:blood* / Q000821:virology; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D009842:Oligopeptides; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma