title: Effect of Bio-Oss with or without platelet-derived growth factor on bone formation by "guided tissue regeneration": a pilot study in rats.
abstract: OBJECTIVE
  To investigate the effect of Bio-Oss with and without the local application of recombinant human platelet-derived growth factor (rhPDGF-BB) on bone formation under Teflon capsules.
  
  
  METHODS
  Eight male, 6-month-old, Wistar strain rats were used in the study. In each animal, the lateral aspect of the mandibular ramus was exposed and small perforations were produced in the bone. A rigid, non-porous hemispherical teflon capsule (diameter 7 mm) was placed on the ramus in both sides of the animals. The capsule placed on the one side of the jaw was filled with Bio-Oss granules soaked in a solution of PDGF-BB (20 microg/capsule) and autogenous blood prior to placement. The capsules placed on the other side of the jaw were filled with Bio-Oss granules soaked in autogenous blood only (controls). Four rats were sacrificed after 3 months and the remaining four after 5 months. Undecalcified sections containing the capsule and surrounding tissues were prepared and analysed in the microscope.
  
  
  RESULTS
  Histologic analysis revealed limited amounts of bone formation. Most of the space underneath the capsules was occupied by Bio-Oss particles surrounded by fibrovascular connective tissue. Given the small sample size statistical analysis was not possible, however, the mean amount of mineralized new bone in the control group (20.8%) appeared to be larger than that in the test group (6.7%). After 5 months the amount of newly formed bone appeared similar in the two groups (23.0% test, 26.0% controls). The Bio-Oss particles occupied between 31.4% and 41.1% of the capsule area at 3 months and between 34.0% and 34.7% at 5 months. Only particles adjacent to the mandibular ramus were incorporated in newly formed bone.
  
  
  CONCLUSIONS
  Limited bone formation was present in the capsules grafted with Bio-Oss with or without the growth factor.
authors.affiliation: Department of Periodontology and Oral Gerontology, Royal Dental College, University of Aarhus, Denmark. b.hack@wanadoo.nl
authors.forename: Natalia
authors.initials: N
authors.lastname: Lioubavina-Hack
authors.forename: Daniela
authors.initials: D
authors.lastname: Carmagnola
authors.forename: Samuel E
authors.initials: SE
authors.lastname: Lynch
authors.forename: Thorkild
authors.initials: T
authors.lastname: Karring
mesh_terms: D000818:Animals; D000077214:Becaplermin; D001672:Biocompatible Materials / Q000627:therapeutic use*; D018786:Bone Substitutes / Q000627:therapeutic use*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D008297:Male; D008334:Mandible / Q000187:drug effects / Q000601:surgery; D008903:Minerals / Q000627:therapeutic use*; D010865:Pilot Projects; D010982:Platelet-Derived Growth Factor / Q000627:therapeutic use*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: C077540:Bio-Oss; D001672:Biocompatible Materials; D018786:Bone Substitutes; D008903:Minerals; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin