title: Evidence that the yeast spindle assembly checkpoint has a target other than the anaphase promoting complex.
abstract: The spindle assembly checkpoint monitors biorientation of chromosomes on the metaphase spindle and inhibits the Anaphase Promoting Complex (APC) specificity factor Cdc20. If APC-Cdc20 is the sole target of the spindle checkpoint, then cells lacking APC and its targets, B-type cyclin and securin, would lack spindle checkpoint function. We tested this hypothesis in yeast cells that are APC-null. Surprisingly, we find that such yeast cells are able to activate the spindle assembly checkpoint, delaying cell cycle progression in G2/M phase. These data suggest that the spindle checkpoint has a non-APC target that can restrain anaphase onset.
authors.affiliation: Department of Genetics, Cell Biology & Development, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Andrews
authors.forename: Laura A
authors.initials: LA
authors.lastname: Díaz-Martínez
authors.forename: Juan F
authors.initials: JF
authors.lastname: Giménez-Abián
authors.forename: Vincent
authors.initials: V
authors.lastname: Guacci
authors.forename: Amit C
authors.initials: AC
authors.lastname: Vas
authors.forename: Duncan J
authors.initials: DJ
authors.lastname: Clarke
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D002455:Cell Division / Q000235:genetics; D019926:Cyclin B / Q000172:deficiency / Q000235:genetics; D016195:G2 Phase / Q000235:genetics; D009687:Nuclear Proteins / Q000172:deficiency / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D064249:Securin; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism*; D043743:Ubiquitin-Protein Ligase Complexes / Q000037:antagonists & inhibitors* / Q000172:deficiency / Q000235:genetics
chemical_list: C577495:CDC20 protein, S cerevisiae; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D019926:Cyclin B; D009687:Nuclear Proteins; C578004:PDS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D064249:Securin; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome