Chemical synthesis of oligonucleotides containing (M+4) guanine.
HPLC MS/MS has shown great potential in the measurement of DNA oxidative damage. Its accuracy depends on the use of multiply isotopically labelled internal standards. In this report, multiply isotopically labelled (M + 4) guanine internal standards were prepared in the form of base, nucleoside, as well as DNA oligomer. To our knowledge, this is the first chemical synthesis of oligomers containing (M + 4) guanine, and we believe that they can be used to develop a procedure that can make further improvement to the existing analytical procedures.
Guo
Ruoling
R
School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK. guor@aston.ac.uk
Zheng
Qinguo
Q
D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D006147:Guanine / Q000737:chemistry*; D013058:Mass Spectrometry; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D006147:Guanine; D004247:DNA; D010100:Oxygen