title: Nuclear magnetic resonance and thermal studies on the interaction between salicylic acid and model membranes.
abstract: DSC and (1H and 31P) NMR measurements are used to investigate the perturbation caused by the keratolytic drug, salicylic acid (SA) on the physicochemical properties of the model membranes. Model membranes (in unilamellar vesicular (ULV) form) in the present studies are prepared with the phospholipids, dipalmitoyl phosphatidylcholine (DPPC), dipalmitoyl phosphatidylethanolamine (DPPE), dipalmitoyl phosphatidic acid (DPPA) and mixed lipid DPPC-DPPE (with weight ratio, 2.5:2.2). These lipids have the same acyl (dipalmitoyl) chains but differed in the headgroup. The molar ratio of the drug to lipid (lipid mixture), is in the range 0 to 0.4. The DSC and NMR results suggest that the lipid head groups have a pivotal role in controlling (i) the behavior of the membranes and (ii) their interactions with SA. In the presence of SA, the main phase transition temperature of (a) DPPE membrane decreases, (b) DPPA membrane increases and (c) DPPC and DPPC-DPPE membranes are not significantly changed. The drug increases the transition enthalpy (i.e., acyl chain order) in DPPC, DPPA and DPPC-DPPE membranes. However, the presence of the drug in DPPC membrane formed using water (instead of buffer), shows a decrease in the transition temperature and enthalpy. In all the systems studied, the drug molecules seem to be located in the interfacial region neighboring the glycerol backbone or polar headgroup. However, in DPPC-water system, the drug seems to penetrate the acyl chain region also.
authors.affiliation: Solid State Physics Division, Bhabha Atomic Research Centre, Mumbai 400 085, India. lata@magnum.barc.ernet.in
authors.forename: Lata
authors.initials: L
authors.lastname: Panicker
authors.forename: K P
authors.initials: KP
authors.lastname: Mishra
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D007641:Keratolytic Agents / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D010712:Phosphatidic Acids / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D020156:Salicylic Acid / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D007641:Keratolytic Agents; D008567:Membranes, Artificial; D010712:Phosphatidic Acids; D010714:Phosphatidylethanolamines; C031599:dipalmitoylphosphatidic acid; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; D020156:Salicylic Acid