Potentiometric sensor for the measurement of Cd2+ transport in yeast and plants.
Research on heavy metals, and especially on transport of Cd(2+), has attracted much interest during the past decade. An optimized Cd(2+)-selective electrode for the continuous potentiometric monitoring of Cd(2+) fluxes in biological systems is presented. The selectivity of the electrode for Cd(2+) was further improved, and it now has very good long-term stability. The utility of this simple and inexpensive method is demonstrated by studying the Cd(2+) transport with model organisms, such as the yeast Saccharomyces cerevisiae and Arabidopsis cell cultures, frequently used in plant science. Its lower detection limits in the presence of commonly used growth media for yeast and plant cells are improved by approximately three orders of magnitude and are 10(-10) and 10(-8)M Cd(2+), respectively. Control experiments using atomic absorption spectrophotometry confirm that the decrease in Cd(2+) activities in the cell cultures is indeed due to the uptake of these metal ions by the cells. Both model systems can be easily transformed; therefore, in combination with the new electrode, they are very promising tools for the investigation of any protein of interest that might be involved in Cd(2+) transport.
Plaza
Sonia
S
Molecular Plant Physiology, Institute of Plant Biology, University of Zurich, Switzerland.
Szigeti
Zsófia
Z
Geisler
Markus
M
Martinoia
Enrico
E
Aeschlimann
Beat
B
Günther
Detlef
D
Pretsch
Ernö
E
D017360:Arabidopsis / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism*; D002413:Cations, Divalent; D017136:Ion Transport; D017736:Ion-Selective Electrodes; D011199:Potentiometry / Q000295:instrumentation / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D002413:Cations, Divalent; D002104:Cadmium