title: Hypercholesterolemia, not metabolic syndrome, related to adhesion of monocytes to cultured endothelium in nondiabetic subjects.
abstract: The interaction of leukocytes and endothelium plays an important role in the development of atherosclerosis. Previous studies found that adhesion of leukocytes to endothelium is greater in subjects with hypercholesterolemia. It is not clear if metabolic syndrome, a contributing risk factor of cardiovascular disease, is related to this adhesion. Therefore, we conducted a study, in which 48 nondiabetic subjects were enrolled, to determine the relationship between leukocyte adhesion and the components of metabolic syndrome. After a 12-hour overnight fast, subjects' fasting blood was obtained for measurement of lipoprotein concentrations and glucose and insulin levels. Results of the number of monocyte adhesion to human umbilical vein endothelial cells were divided into high monocyte adhesion group and low monocyte adhesion group (n=24 in each group). Plasma concentrations of total cholesterol (245 +/- 5 vs 229+/- 4 mg/dL, P=.021) and low-density lipoprotein cholesterol (LDL-C) (162+/- 4 vs 146+/- 3 mg/dL, P=.003) were both higher in the high monocyte adhesion group than in the low monocyte adhesion group. Monocyte adhesion was significantly correlated to plasma concentrations of LDL-C (r=0.407, P=.002) but not to the total cholesterol (r=0.202, P=.085). However, there was no difference in monocyte adhesion to endothelium between subjects with or without metabolic syndrome, based on the modified criteria from the Adult Treatment Panel III of the National Cholesterol Education Program. Insulin resistance index, presented as homeostasis model assessment insulin resistance, and glucose or insulin responses to oral glucose tolerance test were similar between groups. Our study demonstrated that monocyte adhesion to endothelium has a stronger relationship with the plasma concentration LDL-C than with characteristics of metabolic syndrome.
authors.affiliation: Division of Endocrinology and Metabolism, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 407, Taiwan.
authors.forename: I-Te
authors.initials: IT
authors.lastname: Lee
authors.forename: Tsung Min
authors.initials: TM
authors.lastname: Lin
authors.forename: Wen-Jane
authors.initials: WJ
authors.lastname: Lee
authors.forename: Hsiu-Chung
authors.initials: HC
authors.lastname: Ou
authors.forename: Yu-Hon
authors.initials: YH
authors.lastname: Chien
authors.forename: Wen Lieng
authors.initials: WL
authors.lastname: Lee
authors.forename: Yih-Jing
authors.initials: YJ
authors.lastname: Tang
authors.forename: Ching-Hwa
authors.initials: CH
authors.lastname: Yang
authors.forename: Wayne Huey-Herng
authors.initials: WH
authors.lastname: Sheu
mesh_terms: D050197:Atherosclerosis / Q000276:immunology* / Q000378:metabolism; D001786:Blood Glucose; D002448:Cell Adhesion / Q000276:immunology*; D002478:Cells, Cultured; D008078:Cholesterol, LDL / Q000097:blood; D003920:Diabetes Mellitus; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000276:immunology* / Q000378:metabolism; D007333:Insulin Resistance; D008297:Male; D024821:Metabolic Syndrome / Q000276:immunology* / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology* / Q000276:immunology
chemical_list: D001786:Blood Glucose; D008078:Cholesterol, LDL