title: Adiponectin acts as an endogenous antithrombotic factor.
abstract: OBJECTIVE
  Obesity is a common risk factor in insulin resistance and cardiovascular diseases. Although hypoadiponectinemia is associated with obesity-related metabolic and vascular diseases, the role of adiponectin in thrombosis remains elusive.
  
  
  RESULTS
  We investigated platelet thrombus formation in adiponectin knockout (APN-KO) male mice (8 to 12 weeks old) fed on a normal diet. There was no significant difference in platelet counts or coagulation parameters between wild-type (WT) and APN-KO mice. However, APN-KO mice showed an accelerated thrombus formation on carotid arterial injury with a He-Ne laser (total thrombus volume: 13.36+/-4.25 x 10(7) arbitrary units for APN-KO and 6.74+/-2.87x10(7) arbitrary units for WT; n=10; P<0.01). Adenovirus-mediated supplementation of adiponectin attenuated the enhanced thrombus formation. In vitro thrombus formation on a type I collagen at a shear rate of 250 s(-1), as well as platelet aggregation induced by low concentrations of agonists, was enhanced in APN-KO mice, and recombinant adiponectin inhibited the enhanced platelet aggregation. In WT mice, adenovirus-mediated overexpression of adiponectin additionally attenuated thrombus formation.
  
  
  CONCLUSIONS
  Adiponectin deficiency leads to enhanced thrombus formation and platelet aggregation. The present study reveals a new role of adiponectin as an endogenous antithrombotic factor.
authors.affiliation: Department of Hematology and Oncology, Graduate School of Medicine, Osaka University, Suita, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kato
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kashiwagi
authors.forename: Masamichi
authors.initials: M
authors.lastname: Shiraga
authors.forename: Seiji
authors.initials: S
authors.lastname: Tadokoro
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kamae
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Ujiie
authors.forename: Shigenori
authors.initials: S
authors.lastname: Honda
authors.forename: Shigeki
authors.initials: S
authors.lastname: Miyata
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ijiri
authors.forename: Junichiro
authors.initials: J
authors.lastname: Yamamoto
authors.forename: Norikazu
authors.initials: N
authors.lastname: Maeda
authors.forename: Tohru
authors.initials: T
authors.lastname: Funahashi
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kurata
authors.forename: Iichiro
authors.initials: I
authors.lastname: Shimomura
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tomiyama
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Kanakura
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D052242:Adiponectin / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D050197:Atherosclerosis / Q000235:genetics / Q000378:metabolism; D001792:Blood Platelets / Q000502:physiology; D020212:Carotid Artery Injuries / Q000235:genetics / Q000378:metabolism*; D003094:Collagen; D039421:Integrin alpha2 / Q000378:metabolism; D039661:Integrin beta3 / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019007:P-Selectin / Q000378:metabolism; D010974:Platelet Aggregation / Q000502:physiology*; D010976:Platelet Count; D011673:Pulsatile Flow; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface / Q000235:genetics; D013927:Thrombosis / Q000235:genetics / Q000378:metabolism*
chemical_list: D052242:Adiponectin; D039421:Integrin alpha2; D039661:Integrin beta3; D019007:P-Selectin; D054419:Receptors, Adiponectin; D011956:Receptors, Cell Surface; C475419:adiponectin receptor 1, mouse; C475421:adiponectin receptor 2, mouse; D003094:Collagen