Vascular corrosion casting of the uteroplacental and fetoplacental vasculature in mice.
This chapter describes methods for making vascular corrosion casts of the uteroplacental and fetoplacental vasculature of the mouse placenta. A catheter placed in the ascending thoracic aorta of a pregnant mouse permits the introduction of a methyl methacrylate casting compound into the lower body vasculature, including the uterus and placenta. A fine-tipped glass cannula attached to a double-lumen catheter is used to instill the same casting compound in the fetoplacental vessels of mouse placentas. Following polymerization of the casting compound, tissue is digested off of the placental casts using 20% KOH. The washed and dried casts are then available for light or scanning electron microscopy. The methods described have been used to cast the mouse uteroplacental vasculature from 5.5-18.5 d gestation and the fetoplacental vasculature from 12.5 d gestation to term.
Whiteley
Kathie J
KJ
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
Pfarrer
Christiane D
CD
Adamson
S Lee
SL
D000818:Animals; D002404:Catheterization; D016319:Corrosion Casting / Q000379:methods*; D005260:Female; D051379:Mice; D010920:Placenta / Q000098:blood supply* / Q000166:cytology; D021041:Placental Circulation; D011247:Pregnancy