Function of c-Fos-like and c-Jun-like proteins on trichostatin A-induced G2/M arrest in Physarum polycephalum.
The homologs of transcription factors c-Fos and c-Jun have been detected in slime mold Physarum polycephalum during progression of the synchronous cell cycle. Here we demonstrated that c-Fos-like and c-Jun-like proteins participated in G2/M transition by the regulation of the level of Cyclin B1 protein in P. polycephalum. The study of antibody neutralization revealed that interruption of the functions of c-Fos-like and c-Jun-like proteins resulted in G2/M transition arrest, implicating their functional roles in cell cycle control. When G2/M transition was blocked by histone deacetylase inhibitor trichostatin A, changes in c-Fos- and c-Jun-like protein levels, and hyperacetylation of c-Jun-like protein, were observed. The data suggest that in P. polycephalum, c-Fos- and c-Jun-like proteins may be the key factors in the regulation of histone acetylation-related G2/M transition, involving the coordinated expression and hyperacetylation of these proteins.
Li
Xiao-Xue
XX
Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
Lu
Jun
J
Zhao
Yan-Mei
YM
Huang
Bai-Qu
BQ
D000818:Animals; D002478:Cells, Cultured; D019926:Cyclin B / Q000378:metabolism*; D016195:G2 Phase / Q000187:drug effects / Q000502:physiology*; D006877:Hydroxamic Acids / Q000494:pharmacology*; D016839:Physarum polycephalum / Q000187:drug effects / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
D019926:Cyclin B; D006877:Hydroxamic Acids; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; C012589:trichostatin A