Profiling the autoantibody repertoire by serological antigen selection.
The identification of disease related autoantigens targeted by pathogenic T- and B-cell responses is crucial for the development of improved therapies for autoimmune diseases. To identify immunogenic targets recognized by the humoral immune response, we have recently applied a novel and powerful molecular approach, named 'serological antigen selection' (SAS). This method involves the display of a cDNA expression library on filamentous phage and subsequent selection on patient immunoglobulin G (IgG). In the present study, we have cloned a cDNA repertoire from a multiple sclerosis (MS) patient in pVI phage display vectors and performed selections on pooled MS cerebrospinal fluid (CSF) samples immobilized with anti-human IgG. To further streamline this procedure, we report an optimized SAS procedure in which we have successfully established methods for enrichment of MS-specific candidate antigens. In conclusion, the broad applicability of the SAS method makes it a highly promising method for investigating the autoimmune repertoire.
Somers
V
V
Hasselt University, Biomedical Research Institute, and Transnationale Universiteit Limburg, School of Life Sciences, Agoralaan, Building A, B-3590 Diepenbeek, Belgium. veerle.somers@uhasselt.be
Govarts
C
C
Hellings
N
N
Hupperts
R
R
Stinissen
P
P
D000941:Antigens / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000096:biosynthesis* / Q000097:blood; D003115:Colony-Stimulating Factors; D015723:Gene Library; D006801:Humans; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology*; D012698:Serologic Tests
D000941:Antigens; D001323:Autoantibodies; D003115:Colony-Stimulating Factors