title: Complex formations involving both SP-1 and SP-3 at the transcriptional regulatory sequence correlate with the activation of the Keratin 14 gene in human oral squamous cell carcinoma cells.
abstract: We have previously reported that significantly higher levels of Keratin 14 (Ker-14) was observed in oral squamous cell carcinoma (OSCC) and severely dysplastic tissues, whereas this expression was reversed in hyperplasia and in mild to moderate dysplasia. In this study, the mechanism of Keratin 14 activation in oral squamous cell carcinoma (OSCC) cell lines (HSC-2, HSC-3 and Ca9-22) was investigated. Reporter analysis demonstrated that an upstream region (-1759/-1629) accounted for efficient promoter activity. Furthermore, electromobility sift and supershift assay demonstrated that interactions of the SP-1/SP-3 complex at the elements resided in -1737/-1702 and -1680/-1652 and may be essential for this activation in OSCC cells.
authors.affiliation: Department of Biochemistry II, National Defense Medical College, 3-2 Namiki, Tokorozawa-shi, Saitama 359-8513, Japan.
authors.forename: Shuri
authors.initials: S
authors.lastname: Ohkura
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kondoh
authors.forename: Akiyuki
authors.initials: A
authors.lastname: Hada
authors.forename: Masaaki
authors.initials: M
authors.lastname: Arai
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Masanobu
authors.initials: M
authors.lastname: Sindoh
authors.forename: Masayuki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Isamu
authors.initials: I
authors.lastname: Matsumoto
authors.forename: Mikio
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D024202:Electrophoretic Mobility Shift Assay; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053547:Keratin-14; D007633:Keratins / Q000235:genetics*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology; D009154:Mutation; D015345:Oligonucleotide Probes / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D051705:Sp3 Transcription Factor / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: C507540:KRT14 protein, human; D053547:Keratin-14; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D016329:Sp1 Transcription Factor; D051705:Sp3 Transcription Factor; D007633:Keratins; D008156:Luciferases