title: Phospholipase Cdelta4 associates with glutamate receptor interacting protein 1 in testis.
abstract: We reported previously that phospholipase C (PLC) delta4 is required for calcium mobilization in the zona pellucida-induced acrosome reaction in sperm. Here we focused on the function of the C2 domain of PLCdelta4 and report that glutamate receptor-interacting protein1 (GRIP1) was identified as a binding protein of the PLCdelta4-C2 domain on yeast two-hybrid screening. Physiological interaction of GRIP1 with PLCdelta4 in mouse testis was confirmed by immunoprecipitation with anti-PLCdelta4 antibodies and the association seemed to correlate with the maturation stage of sperm. We also determined that a PDZ-binding motif at the C-terminus of the PLCdelta4-C2 domain is responsible for GRIP1 binding, whereas the sixth or seventh PDZ domain of GRIP1 is essential and sufficient for association with the PLCdelta4-C2 domain. These results indicate that PLCdelta4 binds via its C2 domain to the PDZ6 or PDZ7 domain of GRIP1, and that this association may play a role in spermatogenesis.
authors.affiliation: Laboratory of Genome and Biosignal, School of Life Science, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Irino
authors.forename: Manabu
authors.initials: M
authors.lastname: Ichinohe
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nakahara
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Fukami
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002118:Calcium / Q000494:pharmacology; D020013:Calcium Signaling; D002352:Carrier Proteins / Q000378:metabolism*; D002522:Chlorocebus aethiops; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D054803:Phospholipase C delta; D011485:Protein Binding; D011956:Receptors, Cell Surface; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism*; D015044:Zona Pellucida
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C491309:Grip1 protein, mouse; D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D010738:Type C Phospholipases; C518030:PLCD4 protein, human; D054803:Phospholipase C delta; C518031:Plcd4 protein, mouse; D002118:Calcium