MyoD recruits the cdk9/cyclin T2 complex on myogenic-genes regulatory regions.
During skeletal myogenesis, muscle-regulatory factors bHLH and MEF2 promote the expression of muscle-specific genes by recruiting several chromatin-modifying complexes on specific DNA regulatory sequences. A number of MyoD-interacting proteins have been reported, but whether they are recruited to the chromatin of myogenic loci, and the relationship with other chromatin bound proteins is unknown. We show that MyoD recruits cdk9/cyclin T2, together with the histone acetyltransferases p300 and PCAF, and the chromatin remodeling complex SWI/SNF, on promoters and enhancers of muscle-specific genes, and that this event correlates with the acetylation of histone tails, remodeling of chromatin, and phosphorylation of the C-terminal domain (CTD) of the RNA polymerase II at these elements.
Giacinti
Cristina
C
Department of Biology, Sbarro Institute for Cancer Research and Molecular Medicine, College of Science and Technology Temple University, Philadelphia, Pennsylvania 19122, USA.
Bagella
Luigi
L
Puri
Pier Lorenzo
PL
Giordano
Antonio
A
Simone
Cristiano
C
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002843:Chromatin / Q000502:physiology; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D042863:Cyclin-Dependent Kinase 9 / Q000378:metabolism*; D016213:Cyclins / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D024510:Muscle Development / Q000235:genetics*; D018482:Muscle, Skeletal / Q000166:cytology*; D017570:MyoD Protein / Q000502:physiology*; D032446:Myoblasts / Q000378:metabolism; D018008:Myogenin / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D002843:Chromatin; D016213:Cyclins; D017570:MyoD Protein; D018008:Myogenin; D014157:Transcription Factors; D051357:Cyclin-Dependent Kinase 2; D042863:Cyclin-Dependent Kinase 9