title: Imipenem-resistant Achromobacter xylosoxidans carrying blaVIM-2-containing class 1 integron.
abstract: We characterized seven isolates of imipenem-resistant Achromobacter xylosoxidans that were isolated from patients hospitalized in the intensive care unit at a tertiary hospital in Korea during 2001 to 2003. From the analysis with an isoelectric focusing, polymerase chain reaction, and sequencing methods, all isolates were found to produce VIM-2, OXA-30, and chromosomal AmpC beta-lactamase with a pI of 8.4. They showed a similar antibiogram, which were resistant to all tested aminoglycosides as well as beta-lactams including imipenem (16-32 mg/L) and aztreonam (128 mg/L), and a same DNA fingerprinting pattern by random amplified polymorphic DNA analysis, suggesting that these originated from a single clone. From the analysis of integron structure carried by an isolate of A. xylosoxidans CBU1760, bla(VIM-2) was found to be part of a gene cassette carried on a class 1 integron (3.4 kb) containing three aacA4 gene cassettes. This is the first report of bla(VIM-2) in A. xylosoxidans.
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Chungbuk National University, Cheongju, 361-711 Republic of Korea. ksshin@chungbuk.ac.kr
authors.forename: Kyeong Seob
authors.initials: KS
authors.lastname: Shin
authors.forename: Kyudong
authors.initials: K
authors.lastname: Han
authors.forename: Jungnam
authors.initials: J
authors.lastname: Lee
authors.forename: Seung Bok
authors.initials: SB
authors.lastname: Hong
authors.forename: Bo Ra
authors.initials: BR
authors.lastname: Son
authors.forename: Sei Jin
authors.initials: SJ
authors.lastname: Youn
authors.forename: Jungmin
authors.initials: J
authors.lastname: Kim
authors.forename: Hyung Sik
authors.initials: HS
authors.lastname: Shin
mesh_terms: D042441:Achromobacter denitrificans / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D006785:Hospitals, University; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D032023:Integrons / Q000235:genetics*; D007723:Korea / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases