CadC activates pH-dependent expression of the Vibrio vulnificus cadBA operon at a distance through direct binding to an upstream region.
The Vibrio vulnificus cadBA genes were transcribed as a transcriptional operon by a single promoter, P(cadBA), which was activated by CadC in a pH-dependent manner. A direct interaction between CadC and the P(cadBA) DNA was demonstrated, and a CadC binding site centered at -233.5 was mapped by deletion analyses of P(cadBA) and confirmed by a DNase I protection assay.
Rhee
Jee Eun
JE
Department of Food Science and Technology, School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, South Korea.
Kim
Kun-Soo
KS
Choi
Sang Ho
SH
D026905:Amino Acid Transport Systems / Q000235:genetics*; D017920:Antiporters / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002262:Carboxy-Lyases / Q000032:analysis; D018983:DNA Footprinting; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014158:Transcription, Genetic; D041261:Vibrio vulnificus / Q000235:genetics*
D026905:Amino Acid Transport Systems; D017920:Antiporters; D001426:Bacterial Proteins; C463484:CadA protein, Vibrio vulnificus; C088101:CadB protein, Bacteria; C080050:CadC protein, Bacteria; D004269:DNA, Bacterial; D002262:Carboxy-Lyases; C021520:lysine decarboxylase