Evaluation of the carcinogenic potential of clofibrate in the neonatal mouse.
This study was conducted in support of the International Life Sciences Institute (ILSI) alternative carcinogenicity models initiative to evaluate the carcinogenic potential of clofibrate, a nongenotoxic peroxisome proliferator-activated receptor (PPAR) alpha agonist, following oral administration to neonatal mice. Male and female neonatal CD-1 mice were dosed with clofibrate at doses of 100, 250, and 500 mg/kg or with the positive control, diethylnitrosamine (DEN), at 2 mg/kg by oral gavage on days 9 and 16 post birth and observed for approximately 1 year for the development of tumors. Plasma levels of clofibric acid after the second administration increased with dose, but were not dose proportional. Clofibrate administered by gavage on litter days 9 and 16 to neonatal mice at doses of 100, 250, or 500 mg/kg did not produce a carcinogenic effect. The positive control DEN did produce tumors in the liver and lung (single and multiple adenomas and carcinomas) and harderian gland (adenoma) of both sexes. Non-neoplastic lesions related to DEN treatment were confined to myocardial degeneration/fibrosis and testicular interstitial hyperplasia in males, and to glomerulonephrosis and gastritis in both sexes.
Nesfield
Sarah R
SR
GlaxoSmithKline, Safety Assessment, Ware, United Kingdom. sarah.r.nesfield@gsk.com
Williams
Thomas C
TC
Hoivik
Debie J
DJ
Miller
Richard T
RT
Allen
Jane S
JS
Selinger
Krzysztof
K
Rickert
Douglas
D
Santostefano
Michael J
MJ
D000818:Animals; D000831:Animals, Newborn; D015197:Carcinogenicity Tests; D002994:Clofibrate / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D007441:Intubation, Gastrointestinal; D008297:Male; D051379:Mice; D023421:Models, Animal; D020025:Peroxisome Proliferators / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D018570:Risk Assessment; D013997:Time Factors
D020025:Peroxisome Proliferators; D002994:Clofibrate