title: Synthesis of a new pair of fluorescence resonance energy transfer donor and acceptor dyes and its use in a protease assay.
abstract: A new, efficient and very robust fluorescence resonance energy transfer (FRET) system, which can be measured in a normal as well as in a time-resolved mode, was developed and its feasibility demonstrated in a protease assay format.
authors.affiliation: Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstr. 21, D-79104 Freiburg, Germany.
authors.forename: Eva Katharina
authors.initials: EK
authors.lastname: Kainmüller
authors.forename: Eulàlia Pinyol
authors.initials: EP
authors.lastname: Ollé
authors.forename: Willi
authors.initials: W
authors.lastname: Bannwarth
mesh_terms: D002384:Catalysis; D004952:Esters / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006571:Heterocyclic Compounds / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D010447:Peptide Hydrolases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D004952:Esters; D005456:Fluorescent Dyes; D006571:Heterocyclic Compounds; D010447:Peptide Hydrolases