Surfactant displacement of human serum albumin adsorbed on loosely packed self-assembled monolayers: cetyltrimethylammonium bromide versus sodium dodecyl sulfate.
The surfactants sodium dodecyl sulfate (SDS) and cetyltrimethylammonium bromide (CTAB) displace human serum albumin (HSA) from loosely packed self-assembled monolayers (SAM) of hydrophobic alkyl chains by different means. Removal of HSA is of interest because previous work has suggested that the adsorption of HSA to such loosely packed SAMs may be sufficiently tenacious to offer opportunities for surface passivation. While HSA remains on the surface after exposure to SDS and rinsing, no protein remains after exposure to CTAB and rinsing. X-ray reflectivity and X-ray photoelectron spectroscopy measurements indicate that CTAB molecules remain interdigitated in the loosely packed SAM after rinsing, suggesting that CTAB is more effective in removing the HSA because it interacts more strongly with the SAM.
Choi
Eugene J
EJ
Maurice Morton Institute of Polymer Science, University of Akron, Akron, OH 44325-3909, USA.
Foster
Mark D
MD
D000327:Adsorption; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D004563:Electrochemistry; D006801:Humans; D012709:Serum Albumin / Q000493:pharmacokinetics*; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents / Q000737:chemistry
D002593:Cetrimonium Compounds; D012709:Serum Albumin; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D000077286:Cetrimonium