Expression of the Bacillus thuringiensis mosquitocidal toxin Cry11Aa in the aquatic bacterium Asticcacaulis excentricus.
A mosquitocidal aquatic bacterium has been developed by introducing an operon containing the cry11Aa, and p20 genes from Bacillus thuringiensis subsp. israelensis (Bti) into the gram-negative aquatic bacterium Asticcacaulis excentricus. After transformation, the cry11Aa gene was successfully expressed in recombinant A. excentricus under the tac promoter, at the level of 0.04 pg/cell. The recombinant bacteria were toxic to Aedes aegypti larvae with an LC(50) of 6.83 x 10(5) cells/mL. We believe that these bacteria may have potential as genetically engineered microorganisms for the control of mosquito larvae.
Armengol
Gemma
G
Unidad de Biotecnología y Control Biológico, Corporación para Investigaciones Biológicas, Medellín, Colombia AA 7378. gemma.armengol@uab.es
Guevara
Oscar Enrique
OE
Orduz
Sergio
S
Crickmore
Neil
N
D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics* / Q000633:toxicity; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics* / Q000633:toxicity; D042063:Caulobacteraceae / Q000235:genetics*; D003001:Cloning, Molecular; D009033:Culicidae / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000096:biosynthesis / Q000235:genetics* / Q000633:toxicity; D015870:Gene Expression; D006460:Hemolysin Proteins; D007814:Larva / Q000382:microbiology; D009876:Operon / Q000235:genetics; D010572:Pest Control, Biological / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000633:toxicity
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D011994:Recombinant Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis