Design of highly efficient and selective transfer reaction of nitrosyl group to dc and d(M)C resulting in specific deamination.
Nitric oxide (NO) is an important endogenous regulatory molecule, and S-nitrosothiols are believed to play a significant role in NO storage, transport, and delivery. Based on the ability to generate NO in vivo, S-nitrosothiols can be used as therapeutic drugs. In this study, we have developed an innovative method for sequence- and base-specific delivery of NO to a specific site of DNA followed by specific deamination.
Ali
M Monsur
MM
CREST, Japan Science and Technology Agency, Japan.
Nagatsugi
Fumi
F
Nakayama
Shizuka
S
Alam
M Rowshon
MR
Kawasaki
Takeshi
T
Sasaki
Shigeki
S
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry; D003641:Deamination; D006151:Guanosine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009154:Mutation; D009569:Nitric Oxide / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry*; D009603:Nitroso Compounds / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013997:Time Factors
D009603:Nitroso Compounds; D009841:Oligonucleotides; D013438:Sulfhydryl Compounds; D006151:Guanosine; D009569:Nitric Oxide; D004247:DNA; D009584:Nitrogen