[Preventive effect of multi-glycoside of tripterygium Wilfordii Hook. f. on proteinuria and mesangial injury in experimental mesangial proliferative glomerulonephritis].
OBJECTIVE
To observe the preventive effect of multi-glycoside of Tripterygium Wilfordii Hook. f. (GYW) on proteinuria and mesentery injury in experimental mesangial proliferative glomerulonephritis in vivo.


METHODS
The reversible anti-Thyl.1 antibody glomerulo nephritis model of rats was established with monoclonal antibody 1-22-3 and intervened with GTW, and a control group was set up in the same time. Changes of 24h urinary protein excretion, serum creatinine (Scr), blood urea nitrogen (BUN), total plasma protein (TP) and glomerular morphology were observed, and the level of mRNA expression of proliferative factors, including platelet-derived growth factor-BB (PDGF-BB) and transforming growth factor-beta (TGF-beta), in renal tissue was determined.


RESULTS
GTW could inhibit proteinuria and mesangial injury in anti-Thyl. 1 antibody nephritis model. The PDGF-BB and TGF-beta mRNA expression in the anti-Thy1.1 antibody nephritis model rats were increased for 2.84 and 1.64 times respectively to those in the normal control group. GTW could down-regulate the over-expression of PDGF-BB mRNA by 33.1%, it was significantly different to that in the control group (P < 0.05).


CONCLUSIONS
GTW could reduce the proteinuria and inhibit mesangial cells proliferation and extracellular matrix deposition, these effects maybe related to the down-regulating of PDGF-BB mRNA expression.
Wan
Yi-gang
YG
Department of Traditional Chinese Medicine, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing.
Sun
Wei
W
Zhen
Yan-jun
YJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000077214:Becaplermin; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D005260:Female; D005920:Glomerular Mesangium / Q000473:pathology; D015432:Glomerulonephritis, Membranoproliferative / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D011507:Proteinuria / Q000517:prevention & control; D020574:Proto-Oncogene Proteins c-sis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018800:Thy-1 Antigens / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics; D029757:Tripterygium / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D010936:Plant Extracts; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D018800:Thy-1 Antigens; D016212:Transforming Growth Factor beta; C508235:multiglycosidorum tripterygii; D000077214:Becaplermin