Creating new genes by plasmid recombination in Escherichia coli and Bacillus subtilis.
Gene shuffling is a way of creating proteins with interesting new characteristics, starting from diverged sequences. We tested an alternative to gene shuffling based on plasmid recombination and found that Bacillus subtilis efficiently recombines sequences with 4% divergence, and Escherichia coli mutS is more appropriate for sequences with 22% divergence.
Gomez
Ana
A
Eucodis GmbH, Wien, Austria.
Galic
Tatjana
T
Mariet
Jean-François
JF
Matic
Ivan
I
Radman
Miroslav
M
Petit
Marie-Agnès
MA
D001412:Bacillus subtilis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005821:Genetic Techniques; D051719:MutS DNA Mismatch-Binding Protein / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic
D029968:Escherichia coli Proteins; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli