Recommendations for a toxicological screening ERG procedure in laboratory animals.
Electroretinography, using laboratory animals, is a commonly used technique for determining the retinal toxicity of chemical agents. In this paper, guidelines for performing this test are provided. The physiologic basis for visual testing is presented with attention to inter-species differences. Technical aspects of animal recordings are reviewed, including animal preparation, stimulation, signal conditioning, recording and data analysis. Finally, suggested protocols for recording in diurnal and nocturnal species are presented.
Rosolen
Serge G
SG
Clinique Vétérinaire Voltaire, 119 Boulevard Voltaire, 92600, Asnières, France.
Rigaudière
Florence
F
Le Gargasson
Jean-François
JF
Brigell
Mitchell G
MG
D000818:Animals; D004195:Disease Models, Animal; D004596:Electroretinography; D017410:Practice Guidelines as Topic; D012160:Retina / Q000187:drug effects / Q000503:physiopathology*; D012164:Retinal Diseases / Q000139:chemically induced / Q000175:diagnosis* / Q000503:physiopathology; D014116:Toxicology / Q000379:methods