[Effect of cultivation conditions of Streptomyces globisporus 3-1 on landomycin E production].
The producing of antitumor angucycline antibiotic landomycin E has been studied in dynamics of the surface and deep growth of Streptomyces globisporus 3-1 on different full-value media--soya, maize and peptone-yeast media. It is shown that the highest yield of landomycin E is observed on the maize, a bit lower--on soya, and the lowest--on peptone-yeast media independent of the method of growing. The antibiotic stability is affected by alkali pH of the medium. Growth dynamics of the culture in the maize and soya media in the laboratory 2-litre fermenter is characterized by intensive assimilation of glucose, absorption of dissolved oxygen, accumulation of biomass and landomycin E from the 24th to 48th hour of fermentation. The antibiotic is synthesized as primary metabolite reaching its maximum (180-200 mg/l) for the 48th hour of the culture growth. A hypothesis is put forward on the inhibition of the two-component system of signals transduktion by isoflavone genistein, which stimulates synthesis of landomycin E.
Matseliukh
B P
BP
Tymchyk
O V
OV
Matseliukh
A B
AB
D000617:Aminoglycosides / Q000096:biosynthesis*; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D003470:Culture Media; D007700:Kinetics; D013302:Streptomyces / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D000617:Aminoglycosides; D000903:Antibiotics, Antineoplastic; D003470:Culture Media; C113593:landomycin E