Identification of differential genes in ovarian cancer using representational difference analysis of cDNA.
OBJECTIVE
To identify differential genes between normal ovarian epithelium tissue and ovarian epithelial cancer using representational difference analysis of cDNA (cDNA-RDA).


METHODS
cDNA-RDA was performed to identify the differentially expressed sequences between cDNAs from cancer tissue and cDNAs from normal ovarian tissue in the same patient who was in the early stage of ovarian serous cystadenocarcinoma. These differentially expressed fragments were cloned and analyzed, then sequenced and compared with known genes.


RESULTS
Three differentially expressed cDNA fragments were isolated using cDNA from normal ovarian tissue as tester and cDNA from cancer tissue as driver amplicon by cDNA-RDA. DP III-1 and DP III-2 cDNA clone showed significant homology to the cDNA of alpha actin gene; DP III-3 cDNA clone showed significant homology to the cDNA of transgelin gene.


CONCLUSIONS
cDNA-RDA can be used to sensitively identify the differentially expressed genes in ovarian serous cystadenocarcinoma. Ovarian serous cystadenocarcinoma involves alteration of multiple genes.
Chen
Hong
H
Department of Gynecology and Obstetrics, Second Affiliated Hospital, China Medical University, Shenyang 110004.
Wang
Min
M
Wang
Xin-Yan
XY
Gao
Shan
S
Wang
Jun
J
Guan
Xiao-Ming
XM
D000199:Actins / Q000032:analysis / Q000235:genetics; D001483:Base Sequence; D018284:Cystadenocarcinoma, Serous / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D008840:Microfilament Proteins / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000032:analysis / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics*; D010053:Ovary / Q000737:chemistry; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D000199:Actins; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D008840:Microfilament Proteins; D009124:Muscle Proteins; C051615:transgelin