Expression of rice glutamate decarboxylase in Bifidobacterium longum enhances gamma-aminobutyric acid production.
Bifidobacteria are important for the production of fermented dairy products and probiotic formulas but have a low capacity for gamma-aminobutyric acid (GABA) production. To develop a Bifidobacterium strain with an enhanced GABA production, we transformed Bifidobacterium longum with a rice glutamate decarboxylase (OsGADC(-)) gene by electroporation. When the transformed strain was cultured in medium containing monosodium glutamate, the amount of GABA increased significantly compared with those of untransformed Bifidobacterium. Thus, by introducing a plant derived GAD gene, a Bifidobacterium strain has been genetically engineered to produce high levels of GABA from glutamate.
Park
Ki-Bum
KB
Department of Life Science and Technology, Graduate School, Woosuk University, 565-701, Jeonju, Korea.
Ji
Geun-Eog
GE
Park
Myeong-Soo
MS
Oh
Suk-Heung
SH
D001644:Bifidobacterium / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D005968:Glutamate Decarboxylase / Q000096:biosynthesis* / Q000235:genetics; D018698:Glutamic Acid / Q000378:metabolism; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis*
D010940:Plant Proteins; D011994:Recombinant Proteins; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase