Effect of delivery parameters on immunization to ovalbumin following intracutaneous administration by a coated microneedle array patch system.
Immunization to the model antigen ovalbumin was investigated using a novel intracutaneous delivery system consisting of antigen-coated microneedle arrays. The influence of the following parameters on the resulting immune responses was investigated: depth of vaccine delivery, dose of vaccine delivered, density of microneedles on the array, and area of application. The immune response was found to be dose dependent, and mostly independent of depth of delivery, density of microneedles, or area of application. Our studies show that the shortest, most tolerable microneedle arrays can be used for achieving consistent and high antibody titers. Overall, the microneedle array proves to be a very versatile delivery technology, allowing easy and reproducible antigen delivery to skin for efficient vaccination without the use of a needle and syringe.
Widera
Georg
G
ALZA Corporation, Physiological Systems, 1900 Charleston Road, Mountain View, CA 94039-7210, USA.
Johnson
Juanita
J
Kim
Lomi
L
Libiran
Luz
L
Nyam
Kofi
K
Daddona
Peter E
PE
Cormier
Michel
M
D000279:Administration, Cutaneous; D000818:Animals; D000917:Antibody Formation; D004306:Dose-Response Relationship, Immunologic; D016503:Drug Delivery Systems; D005260:Female; D006168:Guinea Pigs; D007114:Immunization; D009339:Needles; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology*; D012867:Skin / Q000276:immunology
D010047:Ovalbumin