Location of the bases modified by M.BcoKIA and M.BcoKIB methylases in the sequence 5 -CTCTTC-3 /5 -GAAGAG-3.
The strain Bacillus coagulans K contains two DNA-methyltransferases, M.BcoKIA and M.BcoKIB, which recognize the sequence 5 -CTCTTC-3 /5 -GAAGAG-3 and possess N4-methylcytosine and N6-methyladenine specificities, respectively. A special construct containing the recognition site of BcoKI and sites of four IIS restriction endonucleases (IIS restriction endonuclease cassette) was designed to locate the nucleotides modified by the methylases. The modified bases were determined as: 5 -m(4)CTCTTC-3 /5 -GAAGAm(6)G-3 .
Svadbina
I V
IV
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. irina@iteb.ru
Matvienko
N N
NN
Zheleznaya
L A
LA
Matvienko
N I
NI
D001430:Bacteriocins; D001483:Base Sequence; D015254:DNA Modification Methylases / Q000737:chemistry / Q000378:metabolism*; D015257:DNA-Cytosine Methylases / Q000737:chemistry / Q000378:metabolism*; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
D001430:Bacteriocins; C114379:coagulin, Bacillus; D015254:DNA Modification Methylases; D015257:DNA-Cytosine Methylases; C508446:M.BcoKIA methylase; C508445:M.BcoKIB methylase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)