title: [Species identification of biological samples by amplifying intron 8 of the TP53 gene].
abstract: OBJECTIVE
  To identify the species of biological samples by amplifying intron 8 of the TP53 gene.
  
  
  METHODS
  Collected the bloodstains or muscle tissues of 14 kinds of common animals and human. DNA were quantified after extraction. The PCR amplification products were analyzed by PAGE and silver staining.
  
  
  RESULTS
  The human and monkey have one amplification band of 460 bp while the eel, fish, frog, duck, rabbit, cat, mouse, cavy, pig, ox and sheep could amplify different unspecial bands. No amplification products were found in chicken and dog.
  
  
  CONCLUSIONS
  The method of species identification by amplifying intron 8 of the TP53 gene is simple and sensitive.
authors.affiliation: Department of Forensic Biology, College of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001801:Blood Stains; D002415:Cats; D002417:Cattle; D002645:Chickens; D004247:DNA / Q000032:analysis* / Q000097:blood / Q000235:genetics; D017931:DNA Primers; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D005554:Forensic Medicine; D005784:Gene Amplification; D016158:Genes, p53; D006801:Humans; D007438:Introns / Q000235:genetics*; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D013045:Species Specificity; D013194:Staining and Labeling
chemical_list: D017931:DNA Primers; D004247:DNA