title: Transcriptional regulation of early transposon elements, an active family of mouse long terminal repeat retrotransposons.
abstract: While early transposon (ETn) endogenous retrovirus (ERV)-like elements are known to be active insertional mutagens in the mouse, little is known about their transcriptional regulation. ETns are transcribed during early mouse embryogenesis in embryonic stem (ES) and embryonic carcinoma (EC) cell lines. Despite their lack of coding potential, some ETns remain transposition competent through their use of reverse transcriptase encoded by a related group of ERVs-MusD elements. In this study, we have confirmed high expression levels of ETn and MusD elements in ES and EC cells and have demonstrated an increase in the copy number of ETnII elements in the EC P19 cell line. Using transient transfections, we have shown that ETnII and MusD LTRs are much more active as promoters in P19 cells than in NIH 3T3 cells, indicating that genomic context and methylation are not the only factors determining endogenous transcriptional activity of ETns. Three sites in the 5' part of the long terminal repeat (LTR) were demonstrated to bind Sp1 and Sp3 transcription factors and were found to be important for high LTR promoter activity in P19 cells, suggesting that as yet unidentified Sp binding partners are involved in the regulation of ETn activity in undifferentiated cells. Finally, we found multiple transcription start sites within the ETn LTR and have shown that the LTR retains significant promoter activity in the absence of its noncanonical TATA box. These findings lend insight into the transcriptional regulation of this family of mobile mouse retrotransposons.
authors.affiliation: Terry Fox Laboratory, British Columbia Cancer Research Centre, 675 West 10th Avenue, Vancouver, British Columbia V5Z 1L3, Canada.
authors.forename: Irina A
authors.initials: IA
authors.lastname: Maksakova
authors.forename: Dixie L
authors.initials: DL
authors.lastname: Mager
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D045744:Cell Line, Tumor; D004251:DNA Transposable Elements / Q000235:genetics*; D020077:Endogenous Retroviruses / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D018626:Retroelements / Q000235:genetics*; D013234:Stem Cells; D016385:TATA Box; D013724:Teratoma; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D004251:DNA Transposable Elements; D018626:Retroelements