Bystin in perineural invasion of prostate cancer.
BACKGROUND
Bystin, Trophinin, and Tastin are component proteins of an adhesion molecule complex that plays a crucial role in the initial attachment of the embryo to the uterus.


METHODS
Profiling of genes differentially expressed in the perineural invasion (PNI) in vitro model by gene microarray analysis showed overexpression of bystin in prostate cancer cells co-cultured with nerves. Validation was performed at the RNA levels using quantitative PCR.


RESULTS
Bystin is overexpressed in cells co-cultured with nerves. Bystin is also present in human prostatic carcinoma (PCa) cells in PNI location in increasing gradient. Bystin is present in the supernatant of the PNI co-culture.


CONCLUSIONS
Their adhesive and invasive functions in the trophoblast suggest that they might also play a role in perineural adhesion. Bystin is, therefore, an important therapeutic target for neurotropic cancers.
Ayala
Gustavo E
GE
Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA. gayala@bcm.tmc.edu
Dai
Hong
H
Li
Rile
R
Ittmann
Michael
M
Thompson
Timothy C
TC
Rowley
David
D
Wheeler
Thomas M
TM
D000818:Animals; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D005727:Ganglia, Spinal; D006801:Humans; D008297:Male; D051379:Mice; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis; D010525:Peripheral Nerves / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D012334:RNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
C112245:BYSL protein, human; D015815:Cell Adhesion Molecules; D012334:RNA, Neoplasm