title: Anoxic media design, preparation, and considerations.
abstract: Exclusion of oxygen from growth media is essential for the growth of anoxic prokaryotes. In general, anaerobic techniques focus on the use of deaerated boiled growth media. Successful enrichment, isolation, and cultivation of anoxic prokaryotes critically depend on the choice of appropriate growth media and incubation conditions. This chapter discusses the requirements of anoxic prokaryotes for growth in the laboratory and different existing methods for their cultivation.
authors.affiliation: Laboratory for Microbiology, Wageningen University, The Netherlands.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Plugge
mesh_terms: D000362:Agar; D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media; D006358:Hot Temperature; D011135:Polysaccharides, Bacterial
chemical_list: D003470:Culture Media; D011135:Polysaccharides, Bacterial; C048288:gellan gum; D000362:Agar