Culture and transfection of human choriocarcinoma cells.
In vitro models for human trophoblasts were initially established more than three decades ago from isolated choriocarcinomas. They have proven to be extremely valuable for the study of the cellular, molecular, and endocrine aspects of human trophoblasts. This chapter describes basic methods for culture and maintenance of the Jeg-3, Jar, and BeWo human choriocarcinoma cell lines as well as an effective paradigm for introducing DNA into the cells.
Wolfe
Michael W
MW
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, USA.
D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D002822:Choriocarcinoma / Q000235:genetics* / Q000473:pathology*; D003470:Culture Media; D005260:Female; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D011247:Pregnancy; D011401:Promoter Regions, Genetic / Q000235:genetics; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000032:analysis
D003470:Culture Media; D001616:beta-Galactosidase