[Determination of amino acids in fu fang banlangen granulae by a pre-column derivatization RP-HPLC method].
OBJECTIVE
To establish a pre-column derivatization reversed-phase high-performance liquid chromatographic method for the determination of amino acids, which was known as the characteristic constituents of Fu Fang Banlangen Granulae.


METHODS
We determined the amino acids in alkalescence by a pre-column derivatization of DNFB. NaAc buffer (pH 6.4)-Acetonitrile (850:150) was used as the mobile phase on a SinoChrom ODS-BP column. The detector was operated at 360 nm.


RESULTS
The method was carried out over the range of 0.627 - 5. 016 microg for Arg and 0.874 - 7.000 microg for Pro. The recoveries were 97.7% with the RSD = 2.0% for Arg and 97.7% with the RSD = 1.5% for Pro.


CONCLUSIONS
The method has good accuracy and repeatability and it can be used for the quality control of Fu Fang Banlangen Granulae.
Wan
Shaohui
S
Pharmaceutical College of Henan University, Kaifeng 475001.
Xu
Mei
M
Wang
Li
L
Kang
Tingguo
T
Zhao
Chunjie
C
D000596:Amino Acids / Q000032:analysis*; D001120:Arginine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D011392:Proline / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D000596:Amino Acids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D001120:Arginine; D011392:Proline