title: Representational difference analysis and real-time PCR for strain-specific quantification of Lactobacillus sobrius sp. nov.
abstract: Lactobacillus sobrius sp. nov., which was recently isolated from the intestine of weaning piglets, has potential probiotic properties. To follow the fate of L. sobrius strain 001T in dietary interventions, a novel and strain-specific quantitative detection procedure was developed. This procedure was based on the isolation of specific genomic fragments from the type strain by representational difference analysis and their detection by real-time PCR. The described strain-specific quantification approach may be used in studies aimed at tracking bacterial strains added to specific environments.
authors.affiliation: Laboratory of Microbiology, Agrotechnology and Food Sciences Group, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. sergey.konstantinov@wur.nl
authors.forename: Sergey R
authors.initials: SR
authors.lastname: Konstantinov
authors.forename: Hauke
authors.initials: H
authors.lastname: Smidt
authors.forename: Willem M
authors.initials: WM
authors.lastname: de Vos
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D041981:Gastrointestinal Tract / Q000382:microbiology; D007778:Lactobacillus / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D019936:Probiotics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013552:Swine / Q000382:microbiology