[Experimental study of compatibility between chondrocytes and allogenic cartilage microparticle acellular tissue matris in vitro].
OBJECTIVE
To construct tissue engineered cartilage using cartilage microparticle acellular tissue matrix (CMACTM) as scaffold.


METHODS
To determine the content of hydroxyproline, glycosaminoglycan and DNA of CMACTM prepared from sheep's articular cartilage with multistep enzymic method, and to analyze CMACTM with gross observation, histology and scanning electron microscopy. Allogenic chondrocytes were mixed with CMACTM and cultured in vitro from 0 to 35 days. Observations through inverted microscope, scanning and transmission electron microscope, quantifications of hydroxyproline, glycosaminoglycan and DNA in the composite, cells adhesion rate were applied to analyze the results.


RESULTS
The diameter of CMACTM was 0.100-0.154 mm, which contain extracellular matrix only. Hydroxyproline, glycosaminoglycan and DNA quantifications in CMACTM were 204.374 +/- 3.120 microg/mg, 18.302 +/- 2.037 microg/mg and 0.042 +/- 0.013 microg/mg respectively. Allogenic chondrocytes enclosed CMACTM tightly, hydroxyproline, glycosaminoglycan and DNA quantifications in the composite of the two formers increased with difference on 7th day compared with that on 0 day, reached to the peaks on 14th day (hydroxyproline, DNA) and on 21st day (glycosaminoglycan), and retained at a high level on the following days. Cells adhesion rate was 92%.


CONCLUSIONS
Allogenic CMACTM possessed satisfactory biocompatibility for chondrocytes and provided a new scaffold for cartilage tissue engineering.
Han
Xue-feng
XF
Department of Plastic Surgery, Second Hospital of Harbin Medical University, Harbin 150086, China.
Yang
Da-ping
DP
Guo
Tie-fang
TF
Fang
Dong-yun
DY
Xu
Xue-wu
XW
Zhang
Ying
Y
D000818:Animals; D002356:Cartilage / Q000166:cytology*; D002358:Cartilage, Articular / Q000166:cytology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D003594:Cytoplasmic Granules; D005109:Extracellular Matrix; D012756:Sheep; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds