title: Transient isolation of Burkholderia multivorans and Burkholderia cenocepacia from a Brazilian cystic fibrosis patient chronically colonized with Burkholderia vietnamiensis.
abstract: Fifteen serial Burkholderia cepacia complex isolates recovered over a period of 4 years from a single cystic fibrosis patient were analysed for genomovar status by means of recA sequence determination, and genetic relatedness by RAPD-PCR. Twelve isolates were assigned as Burkholderia vietnamiensis, two as Burkholderia cenocepacia and one as Burkholderia multivorans. B. vietnamiensis persisted in the airways during 4 years, except in three occasions when B. cenocepacia or B. multivorans were isolated. The patient was chronically colonized by B. vietnamiensis with the RAPD-profile 12 and transiently by the RAPD-profile 15.
authors.affiliation: Disciplina de Microbiologia e Imunologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87/3 degrees andar, Vila Isabel, RJ, 20551-030, Brazil.
authors.forename: Grasiella M V
authors.initials: GM
authors.lastname: Carvalho
authors.forename: Ana Paula D'A
authors.initials: AP
authors.lastname: Carvalho
authors.forename: Tânia W
authors.initials: TW
authors.lastname: Folescu
authors.forename: Laurinda
authors.initials: L
authors.lastname: Higa
authors.forename: Lucia M
authors.initials: LM
authors.lastname: Teixeira
authors.forename: Maria C M
authors.initials: MC
authors.lastname: Plotkowski
authors.forename: Vânia L
authors.initials: VL
authors.lastname: Merquior
authors.forename: Rodolpho M
authors.initials: RM
authors.lastname: Albano
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Marques
mesh_terms: D000293:Adolescent; D001938:Brazil; D019117:Burkholderia / Q000302:isolation & purification*; D042602:Burkholderia cepacia complex / Q000302:isolation & purification; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D008297:Male