Choroid plexus epithelial cells co-express the long and short form of the leptin receptor.
Two types of leptin receptors (Ob-R) are structurally and functionally characterized. The Ob-Ra, also called short form of leptin receptor, and the Ob-Rb, which is functionally coupled to intracellular signalling pathways. In the CNS, the Ob-Ra is mainly present in the choroid plexus and in the blood-brain barrier and it is involved in leptin transport from the periphery to the brain. The Ob-Rb is widely distributed in the hypothalamus and in many peripheral tissues and is coupled to the JAK/STAT signalling pathway. In this work, we describe the presence of both Ob-Ra and Ob-Rb in a cell line of choroid plexus epithelium. In addition, we demonstrate that Ob-Rb in the choroid plexus is functionally coupled to intracellular signalling pathways as leptin induced the phosphorylation of the signal transducer and activator of transduction 3 (STAT3).
Merino
Beatriz
B
Departamento de Farmacología, Tecnología y Desarrollo Farmacéutico, Facultad de Farmacia, Universidad San Pablo-CEU, Madrid, Spain.
Díez-Fernández
Carmen
C
Ruiz-Gayo
Mariano
M
Somoza
Beatriz
B
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D045744:Cell Line, Tumor; D002831:Choroid Plexus / Q000166:cytology*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D020738:Leptin / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D054411:Receptors, Leptin; D050796:STAT3 Transcription Factor / Q000378:metabolism; D012756:Sheep; D013997:Time Factors
D020738:Leptin; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D050796:STAT3 Transcription Factor