title: Comparison of bacterial and phage display peptide libraries in search of target-binding motif.
abstract: Genetic engineering allows modification of bacterial and bacteriophage genes, which code for surface proteins, enabling display of random peptides on the surface of these microbial vectors. Biologic peptide libraries thus formed are used for high-throughput screening of clones bearing peptides with high affinity for target proteins. There are reports of many successful affinity selections performed with phage display libraries and substantially fewer cases describing the use of bacterial display systems. In theory, bacterial display has some advantages over phage display, but the two systems have never been experimentally compared. We tested both techniques in selecting streptavidin-binding peptides from two commercially available libraries. Under similar conditions, selection of phage-displayed peptides to model protein streptavidin proved convincingly better.
authors.affiliation: Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia. mojca.lunder@ffa.uni-lj.si
authors.forename: Mojca
authors.initials: M
authors.lastname: Lunder
authors.forename: Tomaz
authors.initials: T
authors.lastname: Bratkovic
authors.forename: Bojan
authors.initials: B
authors.lastname: Doljak
authors.forename: Samo
authors.initials: S
authors.lastname: Kreft
authors.forename: Uros
authors.initials: U
authors.lastname: Urleb
authors.forename: Borut
authors.initials: B
authors.lastname: Strukelj
authors.forename: Nadja
authors.initials: N
authors.lastname: Plazar
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics*; D001710:Biotin / Q000737:chemistry; D019151:Peptide Library; D011485:Protein Binding; D019809:Streptavidin / Q000378:metabolism*
chemical_list: D019151:Peptide Library; D001710:Biotin; D019809:Streptavidin