Cloning, sequencing and salt induced expression of PEAMT and BADH in oilseed rape (Brassica napus).
Agriculture productivity is severely hampered by soil salinity, drought and other environmental stresses. Studies on stress-resistant plants (halophytes, xerophytes, accumulating plants for specific toxic ions) have illuminated some mechanisms of stress tolerance in plants at metabolic or molecular levels, which gave some clues on how to genetically engineer stress-tolerant crops. With the isolation of more stress-responsive genes, genetic engineering with modified expression of stress responsive genes may be an effective way to produce stress-tolerant crops. In the present report, two genes (PEAMT and BADH) encoding the corresponding key enzymes for choline and glycine betaine (an important osmoprotectant) biosynthesis in plants were isolated in oilseed rape, an important oil crop in the world. Effects of salt stress on their expression were studied with quantitative PCR and their potential use in the genetic engineering of oilseed rape was discussed.
Ye
Chunjiang
C
Huanzhong Agriculture University, Oilseed Rape Research Institute, Wuhan, 430070, P.R. China.
Wu
Suowei
S
Yang
Qingkai
Q
Ma
Chaozhi
C
Yang
Guangsheng
G
Wang
Bin
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D001622:Betaine / Q000378:metabolism; D050728:Betaine-Aldehyde Dehydrogenase / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D029688:Brassica napus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008780:Methyltransferases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D018749:RNA, Plant / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology
D018749:RNA, Plant; D001622:Betaine; D012965:Sodium Chloride; D050728:Betaine-Aldehyde Dehydrogenase; D008780:Methyltransferases; C102844:phosphoethanolamine methyltransferase; D002794:Choline