title: [Analysis of nonylphenol, octylphenol and bisphenol A in animal tissues by liquid chromatography-tandem mass spectrometry with accelerated solvent extraction].
abstract: A comprehensive analytical method based on liquid chromatography-electrospray ionization tandem mass spectrometry with negative ionization mode has been developed for measuring alkylphenols (AP) and bisphenol A (BPA) in animal tissues. Samples of animal tissues were extracted by accelerated solvent extraction with dichloromethane. Sample concentration and purification were performed using an OASIS NH2 solid extraction cartridge. The effects of mobile phase and additives on ionization were assessed. The recoveries for the compounds ranged from 88% to 101% and the relative standard deviations were below 15%. The detection limits of the method under multiple-reaction monitoring mode were 0.3, 0.05 and 0.1 microg/kg for BPA, nonylphenol (NP) and octylphenol (OP), respectively. The contents of NP ranging from 0.49 to 55.98 microg/kg were found in 27 real samples of animal tissues from Beijing market. The results indicate that the endocrine disrupting nonylphenol is ubiquitous in food of animal origin. Nonylphenol was found in all fish samples with concentration levels ranging from 9. 13 to 55.98 microg/kg.
authors.affiliation: Beijing Center for Disease Control and Prevention, Beijing 100013, China. shaob@bjcdc.org
authors.forename: Bing
authors.initials: B
authors.lastname: Shao
authors.forename: Hao
authors.initials: H
authors.lastname: Han
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Zhao
authors.forename: Juan
authors.initials: J
authors.lastname: Meng
authors.forename: Yalu
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D002853:Chromatography, Liquid / Q000379:methods*; D008460:Meat / Q000032:analysis; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; C474055:octylphenol; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds