[Characteristics of virus double-stranded RNA, isolated from microscopic fungi parasitizing on sugar beet].
We have carried out comparative studies of double-stranded RNA (dsRNA) of viral nature isolated from sugar beet leaves and from mycelium of microscopic fungi using different methods such as PAAG electrophoresis and by polymerase chain reaction (PCR). It was shown that the fragments of dsRNA from sugar beet leaves and from mycelium microscopic fungi had the identical electrophoretic pattern and the same size (1.8 and 2.0 kbp). Using PCR technique it was shown, that isolated dsRNA have a common template for amplification. Electron microscopy of PCR-positive mycelium allows us to detect the virus particles of the spherical form with diameter 30-40 nm. The obtained data confirm our previous suppositions, concerning the belonging of isolated dsRNAs (size 1.8 and 2.0 kbp) to new mycovirus targeted a microscopic fungus, instead of beet cryptic viruses.
Mel'nychuk
M D
MD
Spyrydonov
V H
VH
Oleksiienko
I P
IP
D001487:Basidiomycota / Q000648:ultrastructure / Q000821:virology*; D027461:Beta vulgaris / Q000382:microbiology* / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D025282:Mycelium / Q000648:ultrastructure / Q000821:virology; D010942:Plant Viruses / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*
D012330:RNA, Double-Stranded; D012367:RNA, Viral