A novel approach to solid phase chemical synthesis of oligonucleotide mRNA cap analogs.
A novel approach for the synthesis of 5-capped 2'-O-methyloligoribonucleotides on a disulfide-tethered solid support is described. The key step of the synthesis is ZnCl2 promoted coupling of m7GDP imidazolide to a fully deprotected oligonucleotide 5'-phosphate on-support. By this methodology m7G5'pppm2'Apm2'Upm2'Ap has been prepared.
Jemielity
Jacek
J
Department of Biophysics, Institute of Experimental Physics, Warsaw University, Zwirki i Wigury 93, Warsaw 0289, Poland. jacekj@biogeo.uw.edu.pl
Heinonen
Petri
P
Lonnberg
Harri
H
Darzynkiewicz
Edward
E
D002712:Chlorides / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D006153:Guanosine Diphosphate / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D008967:Molecular Biology / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry; D012314:RNA Cap Analogs / Q000138:chemical synthesis* / Q000737:chemistry; D012315:RNA Caps; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D017967:Zinc Compounds / Q000737:chemistry
D002712:Chlorides; D004220:Disulfides; D007093:Imidazoles; D009841:Oligonucleotides; D012314:RNA Cap Analogs; D012315:RNA Caps; D012333:RNA, Messenger; D017967:Zinc Compounds; D006153:Guanosine Diphosphate; C016837:zinc chloride