Estradiol induces PKA activation through the putative membrane receptor in the living hippocampal neuron.
For the past decade, estrogen actions rapid in onset, short in duration through the putative membrane estrogen receptor have attracted considerable attention. Nevertheless, there is so far limited evidence for estrogenic nongenomic regulation of the adenyl cyclase-cAMP-PKA system, especially in the brain. The present study reconfirms that 17beta-estradiol induces membrane-mediated PKA activation with a short latency in a living hippocampal neural cell with use of BSA-conjugated beta-estradiol which does not reach the nuclear estrogen receptor following my previous publication.
Shingo
A S
AS
Hyogo University School of Health Sciences, Hyogo, Japan. ashingo@hyogo-dai.ac.jp
Kito
S
S
D000818:Animals; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004958:Estradiol / Q000378:metabolism* / Q000494:pharmacology; D005470:Fluorometry / Q000379:methods; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000819:agonists / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D011956:Receptors, Cell Surface; D011960:Receptors, Estrogen; D004958:Estradiol; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases