title: Malassezia furfur in infantile seborrheic dermatitis.
abstract: Our objective was to study both incidence and various strains of Malassezia in infantile seborrheic dermatitis (ISD). Sixty infants between 2 weeks and 2 years old with clinical diagnosis of ISD at the Department of Pediatrics, King Chulalongkorn Memorial Hospital from May 2002 to April 2003 were recruited. Malassezia spp. were isolated from cultured skin samples of the patients, genomic DNA was extracted and the ITS1 rDNA region was amplified. The PCR product was examined by agarose gel electrophoresis and DNA sequences were determined. The ITS1 sequences were also subjected to phylogenetic analysis and species identification. ISD is most commonly found in infants below the age of 2 months (64%), followed by those between 2 and 4 months (28%) old. Cultures yielded yeast-like colonies in 15 specimens. PCR yielded 200-bp products (Candida) in 3 patients and 300-bp products (Malassezia furfur) in 12 patients (18%). Sugar fermentation using API 20C aux performed on the three 200-bp PCR products yielded Candida species. M. furfur was the only Malassezia recovered from skin scrapings of children with ISD.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330 Thailand.
authors.forename: Siriwan
authors.initials: S
authors.lastname: Wananukul
authors.forename: Ariya
authors.initials: A
authors.lastname: Chindamporn
authors.forename: Poomjit
authors.initials: P
authors.lastname: Yumyourn
authors.forename: Sunchai
authors.initials: S
authors.lastname: Payungporn
authors.forename: Chanchuree
authors.initials: C
authors.lastname: Samathi
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D002175:Candida / Q000302:isolation & purification; D002675:Child, Preschool; D004271:DNA, Fungal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D012628:Dermatitis, Seborrheic / Q000453:epidemiology / Q000382:microbiology*; D003881:Dermatomycoses / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D005784:Gene Amplification; D006801:Humans; D015994:Incidence; D007223:Infant; D007229:Infant Welfare; D007231:Infant, Newborn; D008290:Malassezia / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013785:Thailand / Q000453:epidemiology
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal