[The dynamic effects of allogenic transplantations with the bladder acellular matrix grafts of rabbits].
OBJECTIVE
To study the dynamic effects of allogenic transplantations with the bladder acellular matrix grafts (BAMG) of rabbits.


METHODS
Hemi-cystectomies were performed in 25 rabbits, and the defects were repaired with BAMG about half bladder size. The rabbits underwent postoperative assessment of bladder function at 8 weeks, including cystometry, vesical volume, vesical compliance and cystography. The allografts were observed by light microscope and electron microscope at 1, 2, 4, 8, 12, 16 weeks after surgery.


RESULTS
Macroscopic observation revealed that BAMG regenerated gradually. All urodynamic results of 8 weeks after surgery were not different statistically as compared with these of preoperation (P > 0.05). Cystography revealed that the morphous of bladder was recovered. Epithelialization and neovascularity occurred accompanied by infiltration of inflammatory cell at 1 week. Smooth muscle cell and stratified epithelium regenerated 2 weeks after grafting. Neural elements formed around smooth muscle bundles as early as 4 weeks. Each component regenerated on the frame of BAMG sequentially. After 16 weeks, it was difficult to delineate the junction between the host bladder and BAMG by histology.


CONCLUSIONS
After allogenic transplantation with rabbits' BAMG, the constitution and function of the allografts regenerate completely and gradually on the frame of BAMG.
Wang
Yong-quan
YQ
Department of Urology, the First Affiliated Hospital of Haerbin Medical University, Haerbin 150001, China.
Gan
Xiu-guo
XG
An
Rui-hua
RH
Zhang
Cheng
C
Wang
Yan
Y
D000818:Animals; D005109:Extracellular Matrix / Q000637:transplantation*; D011817:Rabbits; D012038:Regeneration; D023822:Tissue Engineering; D014184:Transplantation, Homologous; D001743:Urinary Bladder / Q000166:cytology / Q000502:physiology / Q000637:transplantation*