title: Relationship between cytokine gene polymorphisms and graft-versus-host disease after allogeneic stem cell transplantation in a Brazilian population.
abstract: Graft-versus-host disease (GVHD) is the major complication of allogeneic hematopoietic stem cell transplantation (HSCT), and cytokines are recognized as important mediators of GVHD. Polymorphisms in the regulatory regions of several cytokine genes have been associated with a number of immune diseases as well as organ transplant complications. In this study we have investigated the role of tumor necrosis factor-alpha(-308), interleukin (IL)-6(-174), IL-10(-1082, -819, -592), Interferon-gamma(-874), and transforming growth factor-beta1(+869, +915) polymorphisms on HSCT outcome. Donor/recipient genotypes were analyzed by polymerase chain reaction with sequence specific primers (PCR-SSP). Although we have found a small number of low IL-6, a polymorphism at position -174 of the recipient and donor IL-6 gene was associated with the increased incidence of chronic GVHD. Therefore, this study emphasizes the probable potential role of genetic variability of donor and recipient in determining outcome after transplantation.
authors.affiliation: Department of Clinical Analyses, Maringá State University, Av. Colombo, 5790, Maringá, CEP 87020-900, PR, Brazil. jelvisentainer@uem.br
authors.forename: Jeane Eliete
authors.initials: JE
authors.lastname: Laguila Visentainer
authors.forename: Sofia Rocha
authors.initials: SR
authors.lastname: Lieber
authors.forename: Lígia Beatriz
authors.initials: LB
authors.lastname: Lopes Persoli
authors.forename: Sílvia Barbosa
authors.initials: SB
authors.lastname: Dutra Marques
authors.forename: Afonso Celso
authors.initials: AC
authors.lastname: Vigorito
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Penteado Aranha
authors.forename: Kátia Aparecida
authors.initials: KA
authors.lastname: de Brito Eid
authors.forename: Gislaine Borba
authors.initials: GB
authors.lastname: Oliveira
authors.forename: Eliana Cristina
authors.initials: EC
authors.lastname: Martins Miranda
authors.forename: Luciana
authors.initials: L
authors.lastname: Bragotto
authors.forename: Cármino Antonio
authors.initials: CA
authors.lastname: de Souza
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000235:genetics*; D014644:Genetic Variation; D006086:Graft vs Host Disease / Q000235:genetics* / Q000276:immunology / Q000401:mortality; D018380:Hematopoietic Stem Cell Transplantation / Q000401:mortality; D006412:Hematopoietic Stem Cells; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D014184:Transplantation, Homologous
chemical_list: D016207:Cytokines