title: Nucleic acid recovery from complex environmental samples.
abstract: Effective extraction of nucleic acid from environmental samples is an essential starting point in the molecular analysis of microbial communities in the environment. However, there are many different extraction methods in the literature and deciding which one is best suited to a particular sample is very difficult. This article details the important steps and choices in deciding how to extract nucleic acids from environmental samples and gives specific details of one method that has proven very successful at extracting DNA and RNA from a range of different samples.
authors.affiliation: School of Biological Sciences, University of Reading, United Kingdom.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Purdy
mesh_terms: D001433:Bacteriolysis; D018441:Biofilms; D004269:DNA, Bacterial / Q000302:isolation & purification*; D019286:Endopeptidase K; D004783:Environmental Microbiology; D012329:RNA, Bacterial / Q000302:isolation & purification*; D012988:Soil Microbiology; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D019286:Endopeptidase K