title: Specific detection of RNA on ultra-thin sections.
abstract: In this paper, we describe a specific method for ultrastructural detection of RNA. Our method is based in the bromination "in situ" of uridine residues in the RNA, which allows the detection of brominated RNA with specific antibodies against bromo-deoxyuridine. With this method we can achieve high specificity and resolution, and it can be applied to Epon or acrylic resin embedded material.
authors.affiliation: INSTRACAN, Institute of Cancer Research, Juan Chaumel 16, Villena 03400, Alicante, Spain.
authors.forename: Juana
authors.initials: J
authors.lastname: Calabuig
authors.forename: Joaquin
authors.initials: J
authors.lastname: de Juan
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Iborra
mesh_terms: D000906:Antibodies / Q000378:metabolism; D001966:Bromine / Q000737:chemistry; D005453:Fluorescence; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008854:Microscopy, Electron; D008867:Microtomy; D012334:RNA, Neoplasm / Q000378:metabolism / Q000648:ultrastructure*; D012680:Sensitivity and Specificity; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation; D014529:Uridine / Q000276:immunology / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D000906:Antibodies; D012334:RNA, Neoplasm; D014867:Water; D001966:Bromine; D014529:Uridine