title: Tubulin nitration in human gliomas.
abstract: Immunohistochemical and biochemical investigations showed that significant protein nitration occurs in human gliomas, especially in grade IV glioblastomas at the level of astrocytes and oligodendrocytes and neurones. Enhanced alpha-tubulin immunoreactivity was co-present in the same elements in the glioblastomas. Proteomic methodologies were employed to identify a nitrated protein band at 55 kDa as alpha-tubulin. Peptide mass fingerprinting procedures demonstrated that tubulin is nitrated at Tyr224 in grade IV tumour samples but is unmodified in grade I samples and in non-cancerous brain tissue. These results provide the first characterisation of endogenously nitrated tubulin from human tumour samples.
authors.affiliation: Zoological Station Anton Dohrn, Laboratory of Biochemistry and Molecular Biology, Villa Comunale, I-80121 Naples, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Fiore
authors.forename: Carlo
authors.initials: C
authors.lastname: Di Cristo
authors.forename: Gianluca
authors.initials: G
authors.lastname: Monti
authors.forename: Angela
authors.initials: A
authors.lastname: Amoresano
authors.forename: Laura
authors.initials: L
authors.lastname: Columbano
authors.forename: Pietro
authors.initials: P
authors.lastname: Pucci
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Cioffi
authors.forename: Anna
authors.initials: A
authors.lastname: Di Cosmo
authors.forename: Anna
authors.initials: A
authors.lastname: Palumbo
authors.forename: Marco
authors.initials: M
authors.lastname: d'Ischia
mesh_terms: D000368:Aged; D001253:Astrocytes / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D001932:Brain Neoplasms / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014404:Tubulin / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D014404:Tubulin; C002744:3-nitrotyrosine; D014443:Tyrosine