A rapid and reliable solid-phase extraction method for high-performance liquid chromatographic analysis of opium alkaloids from papaver plants.
A rapid and reliable solid-phase extraction method for HPLC analysis of opium alkaloids from Papaver plants was established. Fifty mg of dried and powdered plant sample was extracted with 5 ml of 5% acetic acid for 30 min under sonication. After centrifugation, 3 ml of the supernatant was loaded on a reversed-phase cation-exchange solid-phase extraction cartridge. After seriate washings with 0.1 M hydrochloric acid and methanol, alkaloids were eluted with a mixture of 28% ammonia and methanol (1:19). The eluate was concentrated under nitrogen stream at 40 degrees C and the residue was dissolved in 50% aqueous methanol for high performance liquid chromatographic analysis. With this solid-phase extraction method, the recovery of morphine, codeine, oripavine, thebaine, papaverine, noscapine and sanguinarine was from 99.94 to 112.18% when the standard alkaloids were added to the plant samples. Opium alkaloids of a variety of genus Papaver plants cultivated in a field and phytotron were analyzed by this method.
Yoshimatsu
Kayo
K
Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation, Ibaraki, Japan. yoshimat@nibio.go.jp
Kiuchi
Fumiyuki
F
Shimomura
Koichiro
K
Makino
Yukiko
Y
D000470:Alkaloids / Q000737:chemistry*; D002214:Capsules; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D009892:Opium / Q000737:chemistry*; D010207:Papaver / Q000737:chemistry*; D012997:Solvents
D000470:Alkaloids; D002214:Capsules; D012997:Solvents; D009892:Opium