title: Decreased perivascular fibrosis but not cardiac hypertrophy in ROCK1+/- haploinsufficient mice.
abstract: BACKGROUND
  Rho GTPase and its downstream target, Rho-associated kinase (ROCK), have been implicated in diverse cardiovascular diseases such as cardiac hypertrophy. However, pharmacological inhibitors of ROCK are not entirely specific, nor can they discriminate between the ROCK isoforms ROCK1 and ROCK2. To determine the specific role of ROCK1 in the development of cardiac hypertrophy, we generated ROCK1(+/-) haploinsufficient mice and determined whether cardiac hypertrophy and remodeling are decreased in these mice.
  
  
  RESULTS
  Litters of ROCK1(-/-) mice on C57Bl/6 background were markedly underrepresented, suggesting lethality in utero or postnatally. ROCK1(+/-) mice, however, are viable and fertile with no obvious phenotypic abnormalities. Basal blood pressure, heart rate, and cardiac dimension and function in ROCK1(+/-) mice were similar to those in wild-type (WT) littermates. Infusion of angiotensin II (400 ng.kg(-1).min(-1) for 28 days) or treatment with NG-nitro-L-arginine methyl ester (1 mg/mL in drinking water for 28 days) caused similar increases in systolic blood pressure, left ventricular wall thickness, left ventricular mass, ratio of heart weight to tibial length, and cardiomyocyte size in ROCK1(+/-) mice and WT littermates. In contrast, perivascular fibrosis in hearts was increased to a lesser extent in ROCK1(+/-) mice compared with WT littermates. This was associated with decreased expression of transforming growth factor-beta, connective tissue growth factor, and type III collagen. In addition, perivascular fibrosis induced by transaortic constriction or myocardial infarction was decreased in ROCK1(+/-) mice compared with WT littermates.
  
  
  CONCLUSIONS
  These findings indicate ROCK1 is critical for the development of cardiac fibrosis, but not hypertrophy, in response to various pathological conditions and suggest that signaling pathways leading to the hypertrophic and profibrotic response of the heart are distinct.
authors.affiliation: Vascular Medicine Research Unit, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Rikitake
authors.forename: Naotsugu
authors.initials: N
authors.lastname: Oyama
authors.forename: Chao-Yung C
authors.initials: CY
authors.lastname: Wang
authors.forename: Kensuke
authors.initials: K
authors.lastname: Noma
authors.forename: Minoru
authors.initials: M
authors.lastname: Satoh
authors.forename: Hyung-Hwan
authors.initials: HH
authors.lastname: Kim
authors.forename: James K
authors.initials: JK
authors.lastname: Liao
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D006332:Cardiomegaly / Q000235:genetics*; D005355:Fibrosis; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009203:Myocardial Infarction / Q000235:genetics / Q000473:pathology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency* / Q000235:genetics*; D000644:Quaternary Ammonium Compounds; D014652:Vascular Diseases / Q000235:genetics* / Q000473:pathology; D054460:rho-Associated Kinases
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D000644:Quaternary Ammonium Compounds; D000804:Angiotensin II; C017005:Rockal; D017346:Protein Serine-Threonine Kinases; C515854:Rock1 protein, mouse; C515855:Rock2 protein, mouse; D054460:rho-Associated Kinases; D019331:NG-Nitroarginine Methyl Ester