Kinetic model of oxidation catalyzed by xanthine oxidase-the final enzyme in degradation of purine nucleosides and nucleotides.
A new kinetic model is presented for analysis of experimental data of oxidation process catalyzed by milk xanthine oxidase. The kinetics for two substrates, xanthine and its analog 2-chloroadenine, in a broad pH range (5.8-9.0) are best described by an equation which is a rational function of degree 2:3 and 2:2, respectively.
Banach
Kinga
K
Department of Biophysics, Institute of Experimental Physics, University of Warsaw, Warsaw, Poland.
Bojarska
Elzbieta
E
Kazimierczuk
Zygmunt
Z
Magnowska
Lucyna
L
Bzowska
Agnieszka
A
D000225:Adenine / Q000031:analogs & derivatives / Q000737:chemistry; D055598:Chemical Phenomena; D002621:Chemistry; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008962:Models, Theoretical; D010100:Oxygen / Q000737:chemistry*; D011684:Purine Nucleosides / Q000737:chemistry*; D012044:Regression Analysis; D019820:Xanthine / Q000737:chemistry; D014969:Xanthine Oxidase / Q000737:chemistry*
D011684:Purine Nucleosides; C069484:2-chloroadenine; D019820:Xanthine; D014969:Xanthine Oxidase; D000225:Adenine; D010100:Oxygen