title: Bovine trophoblast cell culture systems: a technique to culture bovine trophoblast cells without feeder cells.
abstract: Bovine trophoblastic cells are the first cells to differentiate during embryogenesis and play pivotal role in morphological and physiological development of the placenta. We have developed culture systems for bovine trophoblast stem cells isolated from in vitro fertilized blastocysts in the absence of feeder cells. These cells continuously proliferate in Dulbecco's modified Eagle's/F12 culture medium supplemented with bovine endometrial fibroblast-conditioned medium. The cells possess epithelial morphology, express cytokeratin, and form dome-like structures (vesicles). Methods for the maintenance, subculture, storage, and measurement of bovine trophoblast stem cell growth are described. The cells exhibit characteristics of bovine trophoblastic stem cells and possess the ability to differentiate into binucleate cells and express placental lactogen, prolactin-related protein-1, pregnancy-associated glycoprotein-1, and interferon tau.
authors.affiliation: Department of Veterinary Medicine, Iwate University, Morioka City, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hashizume
authors.forename: Arata
authors.initials: A
authors.lastname: Shimada
authors.forename: Haruo
authors.initials: H
authors.lastname: Nakano
authors.forename: Toru
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D015870:Gene Expression; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology; D014327:Trophoblasts / Q000166:cytology*
chemical_list: D012333:RNA, Messenger