Emerging approaches to estimate retention factors in high performance liquid chromatography.
The retention factor is one of the most universally used parameters in chromatography. The errors associated with the conventional ways to determine the retention factor of compounds in liquid chromatography are studied and compared with those corresponding to new approaches. The later avoid the use of extra-column time and hold-up time values, which have proven to be tedious and ambiguous. Simulations and real data, used to examine the accuracy of four different approaches (two classic and two new), suggest that the new approaches could be considered more satisfactory than the classic ones.
Bermúdez-Saldaña
José María
JM
Departamento de Química Analítica, Universitat de València, C/Vicente Andrés Estellés s/n, E-46100 Burjassot, Valencia, Spain.
Escuder-Gilabert
Laura
L
Villanueva-Camañas
Rosa María
RM
Medina-Hernández
María José
MJ
Sagrado
Salvador
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results