Comparison of cardiac troponin I levels in serum and sodium-citrate plasma using the ACCESS 2 immunoassay.
The aim of this study was to determine if sodium-citrate plasma is a suitable substitute for serum for cardiac troponin I (cTnI) measurement using the Beckman ACCESS 2 immunoassay. Linear regression analysis of cTnI levels in paired serum and sodium-citrate plasma samples from 80 patients provided a y-intercept of -0.0193 and a slope of 1.044. The correlation was good [r=0.995; 95% confidence interval 1.017 to 1.068, p <0.0001]. The paired results were not significantly different by Student's t-test. Serum and sodium-citrate plasma appear to be interchangeable as samples for cTnI measurement using the ACCESS 2 assay. Furthermore, the use of plasma reduces the turnaround time and may avoid false positive cTnI results due to residual fibrin.
Wang
Yu-Sin
YS
Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
Feng
Chen-Fen
CF
Er
Tze-Kiong
TK
Tsai
Li-Yu
LY
Chen
Chian-Yu
CY
Chang
Shun-Jen
SJ
Chen
Bai-Hsiun
BH
D005189:False Positive Reactions; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D010949:Plasma / Q000737:chemistry*; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry*; D019210:Troponin I / Q000097:blood*
D019210:Troponin I