title: Effects of intranigral vs intrastriatal fetal mesencephalic neural grafts on motor behavior disorders in a rat Parkinson model.
abstract: BACKGROUND
  Numerous experimental and clinical studies have shown that intrastriatal fetal mesencephalic grafts grow, survive, and reinnervate host brain tissue, resulting in partial recovery of motor deficits. In addition, pharmacological evidence indicates that these grafts increase dopamine secretion in lesioned brain. However, to date, no grafting method has completely restored the nigrostriatal pathway, and there is no consensus on optimal graft numbers or locations. This study compared outcomes with multiple striatal grafts vs a single intranigral graft in a rat model of Parkinson disease.
  
  
  METHODS
  Forty-one female Wistar rats weighing 200 to 250 g were used. First, baseline rotational behavior testing with amphetamine injection was done to identify each animal's dominant nigrostriatal pathway (left vs right hemisphere). Some rats then received a unilateral intranigral injection of 6-hydroxydopamine (4 microL [8 microg]) to produce the Parkinson model lesion, and rotational testing was repeated. One group of the lesioned rats received a single intranigral injection of suspended fetal ventral mesencephalic cells (n = 11), and another received multiple intrastriatal grafts of the same type (n = 11).
  
  
  RESULTS
  Both grafted groups showed significant improvement on rotational testing with amphetamine and apomorphine at 6 weeks "postgrafting" (P < .001 for "postlesioning" vs postgrafting results in each of the 2 groups); however, the animals with multiple intrastriatal grafts showed complete recovery from motor asymmetry, whereas the rats with single intranigral grafts showed only partial improvement.
  
  
  CONCLUSIONS
  The findings indicate that multiple intrastriatal grafts result in significantly greater functional improvement than single intranigral grafts in this rat Parkinson model.
authors.affiliation: Department of Physiology, Uludag University School of Medicine, 16059 Bursa, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Goren
authors.forename: Nevzat
authors.initials: N
authors.lastname: Kahveci
authors.forename: Ozhan
authors.initials: O
authors.lastname: Eyigor
authors.forename: Tulin
authors.initials: T
authors.lastname: Alkan
authors.forename: Ender
authors.initials: E
authors.lastname: Korfali
authors.forename: Kasim
authors.initials: K
authors.lastname: Ozluk
mesh_terms: D018663:Adrenergic Agents; D000818:Animals; D017690:Cell Transplantation / Q000379:methods*; D003342:Corpus Striatum / Q000601:surgery*; D004195:Disease Models, Animal; D005260:Female; D016332:Fetal Tissue Transplantation / Q000379:methods*; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology / Q000637:transplantation*; D009043:Motor Activity; D016627:Oxidopamine; D010300:Parkinson Disease / Q000209:etiology / Q000523:psychology / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000601:surgery*
chemical_list: D018663:Adrenergic Agents; D016627:Oxidopamine