[Molecular-genetic analysis of wheat (T. aestivum L.) genome with introgression of Ae. cylindrica Host genetic elements].
Wheat-aegilops hybrid plants Triticum aestivum L. (2n = 42) x Aegilops cylindrica Host (2n = 28) were investigated with using microsatellite markers. In two BC1F9 lines some genome modifications connected with losing DNA fragments of initial variety or appearing of Aegilops genome elements were detected. In some investigated hybrids new amplicons lacking in parental plants were found. Substitution of wheat chromosomes for aegilops chromosomes was not revealed. Analysis of microsatellite loci in BC2F5 plants showed stable introgression of aegilops genetic elements into wheat; elimination of some transferred aegilops DNA fragments in the course of backcrossing; decreasing size of introgressive elements after backcrossing. Introgressive lines were classified according to genome changes.
Galaev
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AV
Sivolap
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IuM
D000483:Alleles; D032461:Chromosomes, Plant / Q000235:genetics*; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D017343:Genes, Plant; D018745:Genome, Plant; D006824:Hybridization, Genetic; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D014908:Triticum / Q000235:genetics*
D018744:DNA, Plant