title: High-throughput cultivation of microorganisms using microcapsules.
abstract: This chapter describes a universal and novel method that provides access to the immense reservoir of untapped microbial diversity by cultivation. This technique uses microcapsules to encapsulate single cells combined with parallel microbial cultivation under low nutrient flux conditions. Under these conditions, single encapsulated cells grow and form microcolonies within the microcapsules. Flow cytometry is used as a sensitive tool to detect growth within the microcapsules. Microcapsules that contain microcolonies (originated from a single encapsulated cell) are sorted individually into microtiter dishes containing organic-rich medium. This high-throughput cultivation can provide more than 10,000 bacterial and fungal isolates per environmental sample.
authors.affiliation: Diversa Corp., San Diego, California 92121, USA.
authors.forename: Karsten
authors.initials: K
authors.lastname: Zengler
authors.forename: Marion
authors.initials: M
authors.lastname: Walcher
authors.forename: Greg
authors.initials: G
authors.lastname: Clark
authors.forename: Imke
authors.initials: I
authors.lastname: Haller
authors.forename: Gerardo
authors.initials: G
authors.lastname: Toledo
authors.forename: Trevin
authors.initials: T
authors.lastname: Holland
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Mathur
authors.forename: Gary
authors.initials: G
authors.lastname: Woodnutt
authors.forename: Jay M
authors.initials: JM
authors.lastname: Short
authors.forename: Martin
authors.initials: M
authors.lastname: Keller
mesh_terms: D001421:Bacteria, Anaerobic / Q000254:growth & development*; D001431:Bacteriological Techniques / Q000295:instrumentation; D002214:Capsules; D005434:Flow Cytometry; D012988:Soil Microbiology
chemical_list: D002214:Capsules