title: Vertical arrays: microarrays of complex mixtures of nucleic acids.
abstract: Vertical arrays are microarrays that have complex mixtures of nucleic acids as array elements, and that are hybridized with single sequence probes. Like dot blots, many different experiments can be spotted on a single vertical array, allowing single genes to be compared across many conditions. Vertical arrays have two additional advantages over dot blots. First, they are printed on glass slides, allowing the use of low-volume, high-concentration hybridization reactions. Second, they can be made using low-complexity representations of the original nucleic acid population. This increases signal-to-noise relative to the usual use of dot blots, wherein the entire complexity of the population is usually spotted. Whereas standard microarrays achieve horizontal coverage of many genes and are repeated to cover many experiments, vertical arrays achieve vertical coverage of many experiments and are repeated to cover many genes. In cases where the number of genes is limited, but the number of experiments is very large, vertical arrays may be advantageous.
authors.affiliation: Department of Molecular Biology and Cancer Genetics, Sidney Kimmel Cancer Center, San Diego, CA, USA.
authors.forename: Rosana
authors.initials: R
authors.lastname: Risques
authors.forename: Gaelle
authors.initials: G
authors.lastname: Rondeau
authors.forename: Martin
authors.initials: M
authors.lastname: Judex
authors.forename: Michael
authors.initials: M
authors.lastname: McClelland
authors.forename: John
authors.initials: J
authors.lastname: Welsh
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D006801:Humans; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012475:Salmonella / Q000378:metabolism; D013997:Time Factors
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D009696:Nucleic Acids; D012313:RNA