title: Detection of Helicobacter pylori in the middle ear fluid of patients with otitis media with effusion.
abstract: OBJECTIVE
  In patients with otitis media with effusion (OME), colonization of the middle ear effusion (MEE) by Helicobacter pylori (HP) was investigated.
  
  
  METHODS
  A prospective nonrandomized study with nonpaired, nonmatched controls. Smear preparations were immunostained with anti-HP antibody and were subjected to Gram staining and Giemsa staining. The rapid urease test (CLO) was done.
  
  
  RESULTS
  Twelve of 15 smears for MEE were positive for HP by immunohistochemistry and 14 by Giemsa that were Gram-negative. In 3 with positive immunohistochemistry, the CLO was positive.
  
  
  CONCLUSIONS
  The results suggest that HP may exist in the MEE of some patients with OME.
authors.affiliation: Department of Otolaryngology, Kobe Japanpost Hospital, Kamitsutsui-dori, Kobe, Japan. smorinakaent@yahoo.co.jp
authors.forename: Setsuko
authors.initials: S
authors.lastname: Morinaka
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tominaga
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakamura
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000453:epidemiology; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D015994:Incidence; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D017817:Nasal Lavage Fluid / Q000382:microbiology*; D010034:Otitis Media with Effusion / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology*; D011446:Prospective Studies; D012016:Reference Values; D017678:Sex Distribution