Hadamard frequency-encoded SOFAST-HMQC for ultrafast two-dimensional protein NMR.
We demonstrate the feasibility of recording (1)H-(15)N correlation spectra of proteins in only one second of acquisition time. The experiment combines recently proposed SOFAST-HMQC with Hadamard-type (15)N frequency encoding. This allows site-resolved real-time NMR studies of kinetic processes in proteins with an increased time resolution. The sensitivity of the experiment is sufficient to be applicable to a wide range of molecular systems available at millimolar concentration on a high magnetic field spectrometer.
Schanda
Paul
P
Institut de Biologie Structurale, Jean-Pierre Ebel C.N.R.S.-C.E.A.-UJF, 41 rue Jules Horowitz, 38027 Grenoble Cedex, France.
Brutscher
Bernhard
B
D016477:Artifacts; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry; D012815:Signal Processing, Computer-Assisted; D025801:Ubiquitin / Q000737:chemistry*
D011506:Proteins; D025801:Ubiquitin