title: Identification of SepCRP analogues in the cuttlefish Sepia officinalis: a novel family of ovarian regulatory peptides.
abstract: In the cuttlefish, Sepia officinalis, the ovary appears to be one of the main sources of regulatory peptides involved in the successive steps of egg-laying. Following the identification of the SepCRP-1, which is a peptide extracted from ovary and involved in egg capsule secretion, investigations were focused on the identification of related peptides. Seven related-Sepia Capsule Releasing Peptides (R-SepCRPs) were identified by means of mass spectrometry and characterized using MS/MS spectra and/or Edman degradation. Finally, primary structures were verified by the comparison of MS/MS spectra from endogenic and synthetic peptides. This new ovarian peptide family exhibits a conserved SLXKD tag involved in the biological activity. LC-MS/MS screening clearly demonstrates that R-SepCRPs are restricted to the female genital tract. Expressed during vitellogenesis, they are released by vitellogenic follicles and full-grown oocytes (FGO) in the genital coelom. Biological activities suggest that R-SepCRPs would be responsible for the storage of FGO before mating and would take part in the mechanical secretion of egg capsule products, as previously described for SepCRP-1.
authors.affiliation: Laboratoire de Biologie et Biotechnologies Marines, UMR 100 IFREMER Physiologie et Ecophysiologie des Mollusques Marins, Université de Caen, 14032 Caen Cedex, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Bernay
authors.forename: Michèle
authors.initials: M
authors.lastname: Baudy-Floc'h
authors.forename: Bruno
authors.initials: B
authors.lastname: Zanuttini
authors.forename: Jean
authors.initials: J
authors.lastname: Gagnon
authors.forename: Joël
authors.initials: J
authors.lastname: Henry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D017124:Conserved Sequence; D005260:Female; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism*; D010058:Oviposition / Q000502:physiology*; D049833:Sepia / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D036341:Intercellular Signaling Peptides and Proteins