title: Increased expression of hypothalamic NADPH-diaphorase neurons in mice with iron supplement.
abstract: Iron deficiency is known as the most important nutritional problem in the world. The loss of appetite is a common characteristic of iron deficiency. Iron-containing heme is required as a cofactor for nitric oxide synthase (NOS) which produces nitric oxide (NO). NOS in the central nervous system has been suggested to regulate food intake. Hence, we examined the expression of hypothalamic NOS at various levels of dietary iron. ICR mice (n = 30) were randomly divided into three groups based on the level of dietary iron and fed experimental diets for 4 weeks: the normal-iron diet group (7 mg/kg diet, n = 10), the low-iron diet group (21 mg/kg diet, n = 10) and the high-iron diet group (42 mg/kg diet, n = 10). Expression of NOS in the paraventricular nucleus (PVN) and lateral hypothalamic area (LHA) of hypothalamus was examined by histochemistry for nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-diaphorase). The high-iron diet mice showed significantly higher staining intensity of NADPH-diaphorase-positive neurons in the PVN and LHA than the normal- and low-iron diet mice.
authors.affiliation: Department of Obesity Management, Graduate School of Obesity Science, Dongduk Women's University, Seoul, South Korea.
authors.forename: Mi-Ja
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Beong Ou
authors.initials: BO
authors.lastname: Lim
authors.forename: Koji
authors.initials: K
authors.lastname: Yamada
authors.forename: Yoongho
authors.initials: Y
authors.lastname: Lim
authors.forename: Soon Ah
authors.initials: SA
authors.lastname: Kang
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects*; D007031:Hypothalamus / Q000166:cytology / Q000201:enzymology*; D007150:Immunohistochemistry; D019266:Iron, Dietary / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D009252:NADPH Dehydrogenase / Q000032:analysis / Q000235:genetics*; D009474:Neurons / Q000201:enzymology*
chemical_list: D019266:Iron, Dietary; D009252:NADPH Dehydrogenase