title: Determination of three bioactive constituents in moutan cortex by capillary electrophoresis with electrochemical detection.
abstract: Capillary electrophoresis with electrochemical detection has been employed for the separation and determination of the three active constituents (paeonol, benzoyloxypaeoniflorin, and oxypaeoniflorin) in traditional Chinese medicine, Moutan Cortex (root cortex of Paeonia suffruticosa Andrews). The effects of several important factors, such as the concentration of running buffer, the separation voltage, the injection time, and the detection potential, were investigated to determine the optimum conditions. The detection electrode was a 300 microm diameter carbon-disc electrode at a working potential of +0.90 V (versus SCE). The three analytes could be well separated within 7 min in a 40 cm length fused-silica capillary at a separation voltage of 12 kV in a 50 mM borate buffer (pH 9.2). The relation between the peak current and the analyte concentration was linear over 3 orders of magnitude with detection limits (S/N = 3) ranging from 0.4 to 0.7 microM for all analytes. The proposed method has been successfully applied to the determination of paeonol, benzoyloxypaeoniflorin, and oxypaeoniflorin in real plant samples with satisfactory assay results.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200032, China. gangchen@fudan.edu.cn
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Luyan
authors.initials: L
authors.lastname: Zhang
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
mesh_terms: D000098:Acetophenones / Q000032:analysis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry* / Q000627:therapeutic use; D004563:Electrochemistry / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D029596:Paeonia; D013729:Terpenes / Q000032:analysis / Q000737:chemistry
chemical_list: D000098:Acetophenones; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D013729:Terpenes; C434676:benzoyloxypaeoniflorin; C038881:moutan cortex; C047593:oxypaeoniflora; C013638:paeonol