Identification of disease markers by differential display: prion disease.
In order to identify molecular markers of prion disease in peripheral tissues, we used the differential display reverse-transcriptase polymerase chain reaction (DDRT-PCR) procedure to compare gene expression in spleens of infected and uninfected mice. In this study, we identified a novel erythroid-specific gene that was differentially expressed as a result of prion infection. We were able to demonstrate that a decrease in the expression levels of this transcript in hematopoietic tissues was a common feature of prion diseases. Our findings suggest a previously unknown role for the blood erythroid lineage in the development of prion diseases and should provide a new focus for research into diagnostic and therapeutic strategies.
Clinton
Michael
M
Department of Gene Expression and Development, Roslin Institute, Scotland, UK.
Miele
Gino
G
Nandi
Sunil
S
McBride
Derek
D
D000818:Animals; D015415:Biomarkers; D015152:Blotting, Northern; D015139:Blotting, Southern; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis / Q000378:metabolism; D041905:Erythroid Cells / Q000166:cytology; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017096:Prion Diseases / Q000175:diagnosis* / Q000378:metabolism*; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution
D015415:Biomarkers; D018076:DNA, Complementary; D012333:RNA, Messenger; D012313:RNA; D004247:DNA