Systemic delivery of full-length C/EBP beta/liposome complex suppresses growth of human colon cancer in nude mice.
C/EBP beta (CCAAT/enhancer-binding protein beta) is an important transcription factor involved in cellular proliferation and differentiation. Overexpression of the full-length C/EBP beta protein results in cellular growth arrest and apoptosis. Using a nonviral liposome as carrier, we delivered the full-length C/EBP beta expression plasmid, pCN, into nude mice bearing CW-2 human colon cancer tumors via tail vein. Southern blots revealed that the major organs and tumors were transfected. Experimental gene therapy showed that a strong suppression of tumor growth was observed in the pCN-treated mice, and such suppression was due to the overexpression of C/EBP beta, leading to the increased apoptosis in tumors of pCN-treated mice. No apparent toxic effects of pCN/liposome complex were observed in the animals. Thus, C/EBP beta has tumor suppression effect in vivo and may be used in gene therapy for cancers.
Sun
Li
L
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Fu
Bei Bei
BB
Liu
Ding Gan
DG
D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000032:analysis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000473:pathology / Q000628:therapy*; D004247:DNA / Q000032:analysis; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000633:toxicity; D006801:Humans; D007275:Injections, Intravenous; D008081:Liposomes / Q000008:administration & dosage / Q000633:toxicity; D051379:Mice; D008819:Mice, Nude
D022782:CCAAT-Enhancer-Binding Protein-beta; D008081:Liposomes; D004247:DNA