title: Injectable and self-curing composites of acrylic/bioactive glass and drug systems. A histomorphometric analysis of the behaviour in rabbits.
abstract: Injectable self-curing systems based on PMMA, phosphate-free bioactive glasses and the drug fosfosal, a phosphate derivative of salicylic acid with analgesic and moderate anti-inflammatory properties, have been tested in vivo to evaluate their biocompatibility. The model consisted of the injection of dough of cement into a defect created in the femur of rabbits, and the cure of the cement in situ after implantation. The biological response was studied in the short and long terms by macroscopic, radiological and histopathological examination, and quantitatively by histomorphometric and statistical analysis considering the most representative variables at the bone-cement interface: cement, bone marrow, newly formed bone and connective tissue. All bioactive formulations presented resorption of the cement at the end of the experiment in contrast to the control of PMMA, due to the presence of resorbable components. The presence or absence of the phosphate group added by the drug fosfosal influenced mainly on the new bone formation process. The cement formulated with bioactive glasses and in absence of fosfosal produced the maximum amount of neoformed bone at 2 weeks, and then it resorbed at 4 weeks to give a higher amount of neoformed bone at the end of the experiment, compared with the formulation containing only fosfosal. The presence of fosfosal and bioactive glass together affected the ossification process strongly. The osseous tissue was produced more gradually but it continuously increased giving rise to a more stable bone at the end of the experiment.
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros, CSIC. C/Juan de la Cierva, 3 28006-Madrid, Spain.
authors.forename: María Aránzazu
authors.initials: MA
authors.lastname: González Corchón
authors.forename: Manuela
authors.initials: M
authors.lastname: Salvado
authors.forename: Basilio José
authors.initials: BJ
authors.lastname: de la Torre
authors.forename: Francisco
authors.initials: F
authors.lastname: Collía
authors.forename: José Antonio
authors.initials: JA
authors.lastname: de Pedro
authors.forename: Blanca
authors.initials: B
authors.lastname: Vázquez
authors.forename: Julio San
authors.initials: JS
authors.lastname: Román
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000737:chemistry; D001843:Bone Cements / Q000737:chemistry; D004343:Drug Implants / Q000008:administration & dosage* / Q000737:chemistry; D005269:Femur / Q000166:cytology / Q000000981:diagnostic imaging / Q000187:drug effects*; D005898:Glass / Q000737:chemistry; D007267:Injections; D008422:Materials Testing; D010755:Organophosphates / Q000008:administration & dosage / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000008:administration & dosage* / Q000737:chemistry; D011817:Rabbits; D011859:Radiography
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; D004343:Drug Implants; D010755:Organophosphates; C000380:fosfosal; D019904:Polymethyl Methacrylate