Detection of chlorinated DNA and RNA nucleosides by HPLC coupled to tandem mass spectrometry as potential biomarkers of inflammation.
Upon inflammation, activated neutrophils secrete myeloperoxidase, an enzyme able to generate hypochlorous acid (HOCl) from hydrogen peroxide and chloride ions. An analytical method, involving HPLC coupled to electrospray tandem mass spectrometry, has been set-up to detect low levels of HOCl-induced nucleic acids lesions, including both ribo and 2'-deoxyribonucleoside derivatives of 8-chloroguanine, 8-chloroadenine and 5-chlorocytosine. Validation of the developed method was achieved using isolated cells treated with HOCl. The method was found to be sensitive enough to allow the measurement of background levels of 5-chloro-2'-deoxycytidine in the DNA of human white blood cells isolated from 7 mL of blood.
Badouard
Carine
C
Laboratoire des Lésions des Acides Nucléiques, DRFMC/SCIB CEA Grenoble, 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France.
Masuda
Mitsuharu
M
Nishino
Hoyoku
H
Cadet
Jean
J
Favier
Alain
A
Ravanat
Jean-Luc
JL
D000225:Adenine / Q000031:analogs & derivatives / Q000032:analysis; D015415:Biomarkers / Q000032:analysis*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D003839:Deoxyadenosines / Q000032:analysis; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000032:analysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D007249:Inflammation / Q000175:diagnosis*; D007962:Leukocytes / Q000737:chemistry; D009705:Nucleosides / Q000032:analysis*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
C506093:8-chloro-2'-deoxyguanosine; C507300:8-chloroadenine; C479978:8-chlorodeoxyadenosine; C507301:8-chloroguanine; D015415:Biomarkers; D003839:Deoxyadenosines; D009705:Nucleosides; D003841:Deoxycytidine; C035826:5-chloro-2'-deoxycytidine; D006147:Guanine; D003596:Cytosine; D003849:Deoxyguanosine; D000225:Adenine; C401182:5-chlorocytosine