title: Phosphatidylinositol transfer protein-alpha in netrin-1-induced PLC signalling and neurite outgrowth.
abstract: Neurite extension is essential for wiring the nervous system during development. Although several factors are known to regulate neurite outgrowth, the underlying mechanisms remain unclear. Here, we provide evidence for a role of phosphatidylinositol transfer protein-alpha (PlTPalpha) in neurite extension in response to netrin-1, an extracellular guidance cue. PlTPalpha interacts with the netrin receptor DCC (deleted in colorectal cancer) and neogenin. Netrin-1 stimulates PlTPalpha binding to DCC and to phosphatidylinositol (5) phosphate [Pl(5)P], increases its lipid-transfer activity and elevates hydrolysis of phosphatidylinositol bisphosphate (PlP2). In addition, the stimulated PIP2 hydrolysis requires PlTPalpha. Furthermore, cortical explants of PlTPalpha mutant mice are defective in extending neurites in response to netrin-1. Commissural neurons from chicken embryos expressing a dominant-negative PlTPalpha mutant show reduced axon outgrowth. Morpholino-mediated knockdown of PlTPalpha expression in zebrafish embryos leads to dose-dependent defects in motor-neuron axons and reduced numbers of spinal-cord neurons. Taken together, these results identify a crucial role for PlTPalpha in netrin-1-induced neurite outgrowth, revealing a signalling mechanism for DCC/neogenin and PlTPalpha regulation.
authors.affiliation: Institute of Molecular Medicine & Genomics and Department of Neurology, Medical College of Georgia, Augusta, GA 30912, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Yu-Qiang
authors.initials: YQ
authors.lastname: Ding
authors.forename: Yan
authors.initials: Y
authors.lastname: Hong
authors.forename: Zhu
authors.initials: Z
authors.lastname: Feng
authors.forename: Sammy
authors.initials: S
authors.lastname: Navarre
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Xi
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zhu
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Wang
authors.forename: S L
authors.initials: SL
authors.lastname: Ackerman
authors.forename: David
authors.initials: D
authors.lastname: Kozlowski
authors.forename: Lin
authors.initials: L
authors.lastname: Mei
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Xiong
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo / Q000166:cytology* / Q000378:metabolism; D000075389:DCC Receptor; D006801:Humans; D050356:Lipid Metabolism / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism / Q000502:physiology; D009414:Nerve Growth Factors / Q000502:physiology*; D000075388:Netrin-1; D016501:Neurites / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D049294:Phospholipid Transfer Proteins / Q000378:metabolism / Q000502:physiology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000378:metabolism / Q000502:physiology*; D015027:Zebrafish / Q000196:embryology / Q000502:physiology; D029961:Zebrafish Proteins
chemical_list: D000075389:DCC Receptor; C501909:DCC protein, human; D008565:Membrane Proteins; C000617351:NTN1 protein, human; D009414:Nerve Growth Factors; C000617353:Ntn1 protein, mouse; D019269:Phosphatidylinositol 4,5-Diphosphate; D049294:Phospholipid Transfer Proteins; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; D029961:Zebrafish Proteins; C091279:neogenin; C047805:neurite-inducing factor; D000075388:Netrin-1