title: Yeast extract from Express Five serum-free medium contains factors at about 35 kDa, essential for growth of Trichoplusia ni insect cells.
abstract: The yeast extract (of unknown origin) present in the commercially available serum-free medium 'Express Five' contains factors ('yeast extract factors') up to 35 kDa which are essential for growth of Trichoplusia ni insect cells. A yeast extract brand lacking these components could not support growth of T. ni cells. However, cell proliferation was restored by adding chromatographic fractions containing the yeast extract factors. The yeast extract factors were not solely responsible for the growth enhancing effect of yeast extract but some other components, which seem to be generally present in yeast extracts, are also required for T. ni proliferation.
authors.affiliation: School of Biotechnology, Department of Bioprocess Technology, Royal Institute of Technology, AlbaNova University Centre, SE-106 91, Stockholm, Sweden.
authors.forename: Ulrika
authors.initials: U
authors.lastname: Eriksson
authors.forename: Lena
authors.initials: L
authors.lastname: Häggström
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D009036:Moths / Q000166:cytology / Q000502:physiology*; D015003:Yeasts / Q000737:chemistry*
chemical_list: D045424:Complex Mixtures; D003470:Culture Media; D005656:Fungal Proteins