Evaluation of the BD Phoenix Automated Microbiology System SMIC/ID panel for identification and antimicrobial susceptibility testing of Streptococcus spp.
The BD Phoenix Automated Microbiology System SMIC/ID panel was evaluated for identification and antimicrobial susceptibility testing (AST) of various streptococci. A group of 97 consecutive clinical isolates of Streptococcus pneumoniae, 23 Streptococcus pyogenes, 24 Streptococcus agalactiae, and 34 viridans streptococci were collected and comparisons made with routine manual methods used in the clinical microbiology laboratory. Overall, in 162 (91%) of 178 isolates, Phoenix identification results demonstrated agreement. For AST results for the 162 isolates that demonstrated identification concordance, the overall essential agreement rate was 98.5%; the category agreement was 94.9%; and the very major error, major error, and minor error rates were 0%, 0.15%, and 5.8%, respectively. Although relatively high minor error rates were observed with S. pneumoniae and beta-lactams, 79.2% of the 77 minor errors were the result of a single log(2) dilution difference. The Phoenix SMIC/ID panel performed favorably and demonstrated the advantages of automation and simple methodology.
Hirakata
Yoichi
Y
Department of Laboratory Medicine, Nagasaki University School of Medicine and Dentistry, Japan. hirakata@net.nagasaki-u.ac.jp
Matsuda
Junichi
J
Nakano
Michiko
M
Hayashi
Tomomi
T
Tozaka
Shusaku
S
Takezawa
Toshiyuki
T
Takahashi
Hiroshi
H
Higashiyama
Yasuhito
Y
Miyazaki
Yoshitsugu
Y
Kamihira
Shimeru
S
Kohno
Shigeru
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001331:Automation; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008826:Microbial Sensitivity Tests; D011933:Reagent Kits, Diagnostic; D013291:Streptococcus / Q000145:classification* / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D011933:Reagent Kits, Diagnostic