Epstein-Barr virus-encoded latent membrane protein 1 modulates cyclin D1 by c-Jun/Jun B heterodimers.
In our recent studies, we found that LMP1 encoded by Epstein-Barr virus could accelerate the formation of active c-Jun/Jun B heterodimer. We studied the regulation of cyclinD1 by c-Jun/Jun B heterodimers by laser scanning confocal influorescence microscopy, Western blot, luciferase activity assay, super-EMSA and flow cytometry in the Tet-on-LMP1 HNE2 cell line, in which LMP1 expression was regulated by Tet-on system. c-Jun/Jun B heterodimers induced by LMP1 could up regulate cyclin D1 promoter activity and expression. Overexpression of cyclin D1 accelerated the progression of cell cycle.
Song
Xin
X
Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha, China.
Tao
Yongguang
Y
Zeng
Liang
L
Yang
Jing
J
Tang
Faqing
F
Lee
Leo M
LM
Gong
Jianping
J
Wu
Qiao
Q
Cao
Ya
Y
D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism; D019281:Dimerization; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D016755:Proto-Oncogene Proteins c-jun / Q000502:physiology*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000502:physiology*
D004273:DNA, Neoplasm; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D016755:Proto-Oncogene Proteins c-jun; D014763:Viral Matrix Proteins; D019938:Cyclin D1; D048031:JNK Mitogen-Activated Protein Kinases