Ghrelin cell density in the gastrointestinal tracts of animal models of human diabetes.
Ghrelin cell density in the gastrointestinal tract of animal models of human diabetes type 1 and 2 was investigated. The animals used were non-obese diabetic (NOD) mice and obese diabetic mice. Ghrelin cells were detected by immunohistochemistry and quantified by computerized image analysis. Ghrelin-immunoreactive cells were detected in all animals studied. They were abundant in the oxyntic mucosa, patchy and few in the duodenum and rare in the colon. The density of ghrelin-immunoreactive cells decreased in diabetic, pre-diabetic NOD mice and in obese diabetic mice as compared to controls, though not statistically significant. It was concluded that the reduced density of ghrelin-immunoreactive cells in animal models of human diabetes type 1 and 2 might explain the slow gastric emptying and slow intestinal transit found in diabetes gastroenteropathy.
Rauma
J
J
Division of Gastroenterology and Hepatology, Department of Molecular and Clinical Medicine, University Hospital, Linköping, Sweden.
Spångeus
A
A
El-Salhy
M
M
D000818:Animals; D002452:Cell Count; D003106:Colon / Q000737:chemistry / Q000473:pathology; D048909:Diabetes Complications / Q000473:pathology / Q000503:physiopathology; D003922:Diabetes Mellitus, Type 1 / Q000473:pathology* / Q000503:physiopathology; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D004386:Duodenum / Q000737:chemistry / Q000473:pathology; D005260:Female; D005746:Gastric Emptying; D005753:Gastric Mucosa / Q000737:chemistry / Q000473:pathology*; D005769:Gastrointestinal Motility; D054439:Ghrelin; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000737:chemistry / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D010295:Parietal Cells, Gastric / Q000737:chemistry / Q000473:pathology; D036361:Peptide Hormones / Q000032:analysis* / Q000502:physiology
D054439:Ghrelin; D036361:Peptide Hormones