title: [Peripheral blood naive T cell level and its T cell receptor Vbeta repertoire usage profile in patients with chronic myelogenous leukemia].
abstract: OBJECTIVE
  To analyze peripheral blood naive T cell level, its T cell receptor (TCR) Vbeta repertoire usage profile and clonality for evaluating the recent thymic output function and the expansion feature of TCR Vbeta subfamily T cells in patients with chronic myelogenous leukemia (CML).
  
  
  METHODS
  Quantitative detection of T-cell receptor excision DNA circles (TRECs) in peripheral blood mononuclear cells (PBMNC) from 20 cases of CML was preformed by real-time PCR (TaqMan) analysis, and TRECs-number in T-cells was calculated from peripheral blood CD3-positive cell rate. The expression and clonality analysis were detected by RT-PCR and Genescan technique in PBMNC from 14 out of the 20 patients. Nine normal individuals served as controls.
  
  
  RESULTS
  A dramatic reduction of TRECs value in patients with CML was detected as compared with that in normal controls. The mean value of TRECs was 0.06 +/- 0.16 copy/1000 CD3(+) cells in CML patients while 6.84 +/- 4.71 copies/1000 CD3(+) cells in normal controls (P < 0.01). The 1 - 12 Vbeta subfamilies were variably expressed in samples from 14 patients. Genescan analysis identified clonal expanded T cells of some Vbeta subfamily from 13 cases. Vbeta3, Vbeta10, Vbeta19, Vbeta21 and Vbeta22 subfamilies clonal T cells were more frequently seen.
  
  
  CONCLUSIONS
  There is a prominent reduction of recent thymic output naive T cells function in CML. The predominant usage and clonal expansion of TCR Vbeta subfamily T cells could be identified, indicating that CML patients have specific immune response to leukemia associated antigen, in spite of their T cell immunodeficiency.
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
authors.forename: Su-xia
authors.initials: SX
authors.lastname: Geng
authors.forename: Yang-qiu
authors.initials: YQ
authors.lastname: Li
authors.forename: Shao-hua
authors.initials: SH
authors.lastname: Chen
authors.forename: Li-jian
authors.initials: LJ
authors.lastname: Yang
authors.forename: Qing-song
authors.initials: QS
authors.lastname: Yin
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Wu
authors.forename: Xue-li
authors.initials: XL
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D019674:Genes, T-Cell Receptor beta / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics* / Q000276:immunology; D008297:Male; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
chemical_list: D011948:Receptors, Antigen, T-Cell