A tRNA(Glu) that uncouples protein and tetrapyrrole biosynthesis.
Glu-tRNA is either bound to elongation factor Tu to enter protein synthesis or is reduced by glutamyl-tRNA reductase (GluTR) in the first step of tetrapyrrole biosynthesis in most bacteria, archaea and in chloroplasts. Acidithiobacillus ferrooxidans, a bacterium that synthesizes a vast amount of heme, contains three genes encoding tRNA(Glu). All tRNA(Glu) species are substrates in vitro of GluRS1 from A. ferrooxidans.Glu-tRNA(3)(Glu), that fulfills the requirements for protein synthesis, is not substrate of GluTR. Therefore, aminoacylation of tRNA(3)(Glu) might contribute to ensure protein synthesis upon high heme demand by an uncoupling of protein and heme biosynthesis.
Levicán
Gloria
G
Programa de Biologia Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 838-0453, Chile.
Katz
Assaf
A
Valenzuela
Patricio
P
Söll
Dieter
D
Orellana
Omar
O
D042763:Acidithiobacillus / Q000235:genetics / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D005798:Genes, Bacterial / Q000502:physiology; D005975:Glutamate-tRNA Ligase / Q000378:metabolism; D010444:Peptide Elongation Factor Tu / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics; D012352:RNA, Transfer, Glu / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D045725:Tetrapyrroles / Q000096:biosynthesis*; D046249:Transfer RNA Aminoacylation
D012352:RNA, Transfer, Glu; D045725:Tetrapyrroles; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase; D010444:Peptide Elongation Factor Tu; D005975:Glutamate-tRNA Ligase