title: Anti-HBV immune responses in rhesus macaques elicited by electroporation mediated DNA vaccination.
abstract: Electroporation has been shown to be an effective method to improve the efficiency of gene expression and the immunogenicity of DNA vaccines. In order to optimize the procedure and test for its efficacy in more clinically relevant large animal models, we examined the detailed immune responses in rhesus macaques after vaccination intramuscularly with electroporation using the plasmid encoding for HBV preS(2)-S antigen and an adjuvant plasmid encoding for hIL-2 and hIFN-gamma. Several important factors were examined, including the dose response relationships, the effect of various prime and boost regimens, and different combinations of electro-pulse parameters. The immune responses were closely followed for more than a year. The results showed that in rhesus macaques, electroporation can significantly enhance the immunogenicity of the DNA vaccines, resulting in greatly improved antibody responses as well as peptide-stimulated IFN-gamma producing T cell responses. In addition, we also reported the different antibody response behaviors resulted from different electro-pulse parameters. The detailed data would be useful to suggest possible optimization strategies for better DNA vaccine efficacy.
authors.affiliation: School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: Yong-Gang
authors.initials: YG
authors.lastname: Zhao
authors.forename: Baowei
authors.initials: B
authors.lastname: Peng
authors.forename: Hongwei
authors.initials: H
authors.lastname: Deng
authors.forename: Guangming
authors.initials: G
authors.lastname: Chen
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Yang
authors.forename: Ming
authors.initials: M
authors.lastname: Shao
authors.forename: Huili
authors.initials: H
authors.lastname: Lu
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.forename: Jinliang
authors.initials: J
authors.lastname: Peng
authors.forename: Long
authors.initials: L
authors.lastname: Xu
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018274:Electroporation; D005260:Female; D006510:Hepatitis B Antibodies / Q000097:blood*; D017325:Hepatitis B Vaccines / Q000276:immunology*; D008253:Macaca mulatta; D008297:Male; D008969:Molecular Sequence Data; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D006510:Hepatitis B Antibodies; D017325:Hepatitis B Vaccines; D019444:Vaccines, DNA