title: Arp2/3 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function.
abstract: RNA interference silencing of up to 90% of Arp3 protein expression, a major subunit of the Arp2/3 complex, proportionately decreases the intracellular motility of Listeria monocytogenes and actin nucleation activity ascribable to the Arp2/3 complex in mouse embryonic fibroblasts. However, the Arp2/3-deficient cells exhibit unimpaired lamellipodial actin network structure, translational locomotion, spreading, actin assembly, and ruffling responses. In addition, Arp3-silenced cells expressing neural Wiskott-Aldrich syndrome protein-derived peptides that inhibit Arp2/3 complex function in wild-type cells retained normal PDGF-induced ruffling. The Arp2/3 complex can be dispensable for leading-edge actin remodeling.
authors.affiliation: Division of Hematology, Brigham and Women's Hospital, and Department of Medicine, Harvard Medical School, One Blackfan Circle, Boston, MA 02115, USA.
authors.forename: Alessia
authors.initials: A
authors.lastname: Di Nardo
authors.forename: Gregor
authors.initials: G
authors.lastname: Cicchetti
authors.forename: Hervé
authors.initials: H
authors.lastname: Falet
authors.forename: John H
authors.initials: JH
authors.lastname: Hartwig
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Stossel
authors.forename: David J
authors.initials: DJ
authors.lastname: Kwiatkowski
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000235:genetics / Q000502:physiology*; D000199:Actins / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology; D020868:Gene Silencing; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D034622:RNA Interference
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D010982:Platelet-Derived Growth Factor