title: Novel way of capping mRNA trimer and studies of its interaction with human nuclear cap-binding complex.
abstract: Binding of mRNA 5' cap by the nuclear cap-binding complex (CBC) is crucial for a wide variety of mRNA metabolic events. The interaction involving the CBP20 subunit of CBC is mediated by numerous hydrogen bonds and by stacking of the tyrosine sidechains with two first bases of the capped mRNA. To examine a possible role of a longer mRNA chain in the CBC-cap recognition, we have synthesized an mRNA tetramer using a novel way of capping an RNA trimer and determined its affinity for CBC by fluorescence titration.
authors.affiliation: Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warsaw, Poland.
authors.forename: Remigiusz
authors.initials: R
authors.lastname: Worch
authors.forename: Janusz
authors.initials: J
authors.lastname: Stepinski
authors.forename: Anna
authors.initials: A
authors.lastname: Niedzwiecka
authors.forename: Marzena
authors.initials: M
authors.lastname: Jankowska-Anyszka
authors.forename: Catherine
authors.initials: C
authors.lastname: Mazza
authors.forename: Stephen
authors.initials: S
authors.lastname: Cusack
authors.forename: Ryszard
authors.initials: R
authors.lastname: Stolarski
authors.forename: Edward
authors.initials: E
authors.lastname: Darzynkiewicz
mesh_terms: D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D008956:Models, Chemical; D039502:Nuclear Cap-Binding Protein Complex / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012315:RNA Caps / Q000737:chemistry*; D012322:RNA Precursors; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D046911:Macromolecular Substances; D039502:Nuclear Cap-Binding Protein Complex; D012315:RNA Caps; D012322:RNA Precursors; D012333:RNA, Messenger; D012261:Ribonucleoproteins