title: [Expression of macrophage inflammatory protein-1alpha, a disintegrin-like and metalloproteinase 8 and 12, and CD68 protein in giant cell lesions of jaw and giant cell tumors of long bone].
abstract: OBJECTIVE
  To detect the expression of macrophage inflammatory protein-1alpha (MIP-1alpha), a disintegrin-like and metalloproteinase (ADAM) 8 and 12 and CD68 protein in giant cell lesions of jaw and giant cell tumors of long bone, and to study their effects on the histogenesis of giant cells in such lesions.
  
  
  METHODS
  MIP-1alpha, ADAM8, ADAM12 and CD68 were detected by immunohistochemistry in 24 paraffin-embedded specimens of central giant cell lesions of jaw and giant cell tumors respectively.
  
  
  RESULTS
  MIP-1alpha positive signal was located in blood vessels and bone. ADAM8, ADAM12 and CD68 positive signals were located in the cell membrane and cytoplasm of all multinucleated giant cells and some round mononuclear cells in the lesions. In addition, some spindle mononuclear stromal cells were positive for ADAM12 in both lesions.
  
  
  CONCLUSIONS
  Multinucleated giant cells probably originate from CD68-postive round mononuclear cells, which are recruited from monocyte-macrophage system by chemokines, such as MIP-1alpha, followed by cell fusion mediated by ADAM8 and ADAM12.
authors.affiliation: Department of Oral Pathology, School of Stomatology, Peking University, Beijing 100081, China.
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Meng
authors.forename: Shi-feng
authors.initials: SF
authors.lastname: Yu
authors.forename: Min
authors.initials: M
authors.lastname: Lu
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Han
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism*; D000072199:ADAM12 Protein; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D002462:Cell Membrane / Q000378:metabolism; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D003593:Cytoplasm / Q000378:metabolism; D018212:Giant Cell Tumor of Bone / Q000378:metabolism* / Q000473:pathology; D015726:Giant Cells / Q000378:metabolism; D006101:Granuloma, Giant Cell / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007571:Jaw Diseases / Q000378:metabolism / Q000473:pathology; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D000097382:CD68 Molecule
chemical_list: D051722:ADAM Proteins; D000072199:ADAM12 Protein; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins; D008565:Membrane Proteins; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C000606480:ADAM12 protein, human; C068490:ADAM8 protein, human