title: Proteomic analysis of sera from hepatocellular carcinoma patients after radiofrequency ablation treatment.
abstract: Comparative proteomic analysis was used to search for characteristic alterations in the sera of hepatocellular carcinoma (HCC) patients who had undergone curative radiofrequency ablation treatment. Serum samples collected from eight patients before and after treatment were subjected to 2-DE. Eighty-eight protein spots differentially expressed with the treatment were selected by clustering analysis, and the proteins were identified by MS based on MALDI-TOF/TOF analysis and public database searches. The statistical analysis suggested that four proteins decreased after treatment (pro-apolipoprotein, alpha2-HS glycoprotein, apolipoprotein A-IV precursor, and PRO1708/PRO2044, which is the carboxy terminal fragment of albumin) and that seven proteins were increased after treatment, including leucine-rich alpha2-glycoprotein and alpha1-antitrypsin. These data facilitate the identification of differentially expressed proteins that are involved in HCC carcinogenesis and provide candidate biomarkers for the development of diagnostic and therapeutic tools.
authors.affiliation: Department of Gastroenterology, Graduate School of Medicine, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kawakami
authors.forename: Yujin
authors.initials: Y
authors.lastname: Hoshida
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kanai
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tateishi
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Ikenoue
authors.forename: Shuntaro
authors.initials: S
authors.lastname: Obi
authors.forename: Shinpei
authors.initials: S
authors.lastname: Sato
authors.forename: Takuma
authors.initials: T
authors.lastname: Teratani
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Shiina
authors.forename: Takao
authors.initials: T
authors.lastname: Kawabe
authors.forename: Takamasa
authors.initials: T
authors.lastname: Suzuki
authors.forename: Naoya
authors.initials: N
authors.lastname: Hatano
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Masao
authors.initials: M
authors.lastname: Omata
mesh_terms: D001054:Apolipoproteins A / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000601:surgery; D017115:Catheter Ablation; D015180:Electrophoresis, Gel, Two-Dimensional; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood* / Q000601:surgery; D011498:Protein Precursors / Q000097:blood; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D060749:alpha-2-HS-Glycoprotein
chemical_list: C556354:AHSG protein, human; D001054:Apolipoproteins A; D014408:Biomarkers, Tumor; D001798:Blood Proteins; D006023:Glycoproteins; C015246:LRG1 protein, human; D011498:Protein Precursors; D060749:alpha-2-HS-Glycoprotein; C022205:apolipoprotein A-IV