title: Genome comparison of Pseudomonas aeruginosa large phages.
abstract: Pseudomonas aeruginosa phage EL is a dsDNA phage related to the giant phiKZ-like Myoviridae. The EL genome sequence comprises 211,215 bp and has 201 predicted open reading frames (ORFs). The EL genome does not share DNA sequence homology with other viruses and micro-organisms sequenced to date. However, one-third of the predicted EL gene products (gps) shares similarity (Blast alignments of 17-55% amino acid identity) with phiKZ proteins. Comparative EL and phiKZ genomics reveals that these giant phages are an example of substantially diverged genetic mosaics. Based on the position of similar EL and phiKZ predicted gene products, five genome regions can be delineated in EL, four of which are relatively conserved between EL and phiKZ. Region IV, a 17.7 kb genome region with 28 predicted ORFs, is unique to EL. Fourteen EL ORFs have been assigned a putative function based on protein similarity. Assigned proteins are involved in DNA replication and nucleotide metabolism (NAD+-dependent DNA ligase, ribonuclease HI, helicase, thymidylate kinase), host lysis and particle structure. EL-gp146 is the first chaperonin GroEL sequence identified in a viral genome. Besides a putative transposase, EL harbours predicted mobile endonucleases related to H-N-H and LAGLIDADG homing endonucleases associated with group I intron and intein intervening sequences.
authors.affiliation: Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, B-3001 Leuven, Belgium. kirsten.hertveldt@biw.kuleuven.be
authors.forename: Kirsten
authors.initials: K
authors.lastname: Hertveldt
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
authors.forename: Elena
authors.initials: E
authors.lastname: Pleteneva
authors.forename: Natalia
authors.initials: N
authors.lastname: Sernova
authors.forename: Lidia
authors.initials: L
authors.lastname: Kurochkina
authors.forename: Roman
authors.initials: R
authors.lastname: Korchevskii
authors.forename: Johan
authors.initials: J
authors.lastname: Robben
authors.forename: Vadim
authors.initials: V
authors.lastname: Mesyanzhinov
authors.forename: Victor N
authors.initials: VN
authors.lastname: Krylov
authors.forename: Guido
authors.initials: G
authors.lastname: Volckaert
mesh_terms: D000595:Amino Acid Sequence; D004261:DNA Replication / Q000235:genetics; D004720:Endonucleases / Q000378:metabolism; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D047668:Inteins; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017105:Pseudomonas Phages / Q000235:genetics* / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000648:ultrastructure / Q000821:virology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014775:Virus Activation
chemical_list: D014764:Viral Proteins; D004720:Endonucleases