Distribution of 10 periodontal bacteria in saliva samples from Japanese children and their mothers.
OBJECTIVE
We analyzed the distribution of 10 periodontal bacteria species (Porphyromonas gingivalis, Tannerella forsythensis, Prevotella intermedia, Prevotella nigrescens, Campylobacter rectus, Eikenella corrodens, Actinobacillus actinomycetemcomitans, Capnocytophaga ochracea, Capnocytophaga sputigena, and Treponema denticola) in children, and then compared their distribution in those children and their mothers, with special attention given to three of the species known as the red complex (P. gingivalis, T. forsythensis, and T. denticola) whose presence has been shown to be associated with conditions related to periodontal diseases.


METHODS
One hundred thirteen pairs of children and their mothers were randomly selected from patients treated at the Pedodontic Clinic of Osaka University Dental Hospital. Saliva samples were taken at the second visit prior to receiving professional tooth brushing instruction. Genomic DNA was extracted from each saliva sample, followed by a polymerase chain reaction assay with species-specific sets of primers.


RESULTS
A. actinomycetemcomitans was the most frequently detected species in the mothers, followed by C. sputigena, P. gingivalis, and T. forsythensis, while C. sputigena had the highest detection rate, followed by A. actinomycetemcomitans and T. denticola in the children. The detection rate of the red complex species in children whose mothers possessed the same species was significantly higher than in those whose mothers did not possess them.


CONCLUSIONS
Our results indicate a correlation between the presence of periodontal bacteria in children and their mothers, while the presence of red complex bacteria in children was highly associated with that in their mothers.
Tamura
Kiyoko
K
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1-8 Yamada-oka, Suita, Osaka 565-0871, Japan.
Nakano
Kazuhiko
K
Hayashibara
Tetsuyuki
T
Nomura
Ryota
R
Fujita
Kazuyo
K
Shintani
Seikou
S
Ooshima
Takashi
T
D000293:Adolescent; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics; D001419:Bacteria / Q000235:genetics*; D041963:Bacteroidetes / Q000235:genetics; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D007564:Japan; D008297:Male; D008875:Middle Aged; D009035:Mothers; D010518:Periodontitis / Q000382:microbiology*; D010519:Periodontium / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D016966:Porphyromonas gingivalis / Q000235:genetics; D012044:Regression Analysis; D012463:Saliva / Q000382:microbiology*; D048373:Treponema denticola / Q000235:genetics
D004269:DNA, Bacterial