Detection of Helicobacter pylori in the middle ear fluid of patients with otitis media with effusion.
OBJECTIVE
In patients with otitis media with effusion (OME), colonization of the middle ear effusion (MEE) by Helicobacter pylori (HP) was investigated.


METHODS
A prospective nonrandomized study with nonpaired, nonmatched controls. Smear preparations were immunostained with anti-HP antibody and were subjected to Gram staining and Giemsa staining. The rapid urease test (CLO) was done.


RESULTS
Twelve of 15 smears for MEE were positive for HP by immunohistochemistry and 14 by Giemsa that were Gram-negative. In 3 with positive immunohistochemistry, the CLO was positive.


CONCLUSIONS
The results suggest that HP may exist in the MEE of some patients with OME.
Morinaka
Setsuko
S
Department of Otolaryngology, Kobe Japanpost Hospital, Kamitsutsui-dori, Kobe, Japan. smorinakaent@yahoo.co.jp
Tominaga
Masayuki
M
Nakamura
Hiroyuki
H
D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000453:epidemiology; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D015994:Incidence; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D017817:Nasal Lavage Fluid / Q000382:microbiology*; D010034:Otitis Media with Effusion / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology*; D011446:Prospective Studies; D012016:Reference Values; D017678:Sex Distribution