Decalactone production by Yarrowia lipolytica under increased O2 transfer rates.
Yarrowia lipolytica converts methyl ricinoleate to gamma-decalactone, a high-value fruity aroma compound. The highest amount of 3-hydroxy-gamma-decalactone produced by the yeast (263 mg l(-1)) occurred by increasing the k(L)a up to 120 h(-1) at atmospheric pressure; above it, its concentration decreased, suggesting a predominance of the activity of 3-hydroxyacyl-CoA dehydrogenase. Cultures were grown under high-pressure, i.e., under increased O(2) solubility, but, although growth was accelerated, gamma-decalactone production decreased. However, by applying 0.5 MPa during growth and biotransformation gave increased concentrations of dec-2-en-4-olide and dec-3-en-4-olide (70 mg l(-1)).
Aguedo
M
M
Departamento de Engenharia Biológica, Campus de Gualtar, 4710-057, Braga, Portugal.
Gomes
N
N
Garcia
E Escamilla
EE
Waché
Y
Y
Mota
M
M
Teixeira
J A
JA
Belo
I
I
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D001711:Biotransformation / Q000502:physiology; D007783:Lactones / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D012277:Ricinoleic Acids / Q000378:metabolism*; D025062:Yarrowia / Q000254:growth & development*
D007783:Lactones; D012277:Ricinoleic Acids; C109757:decan-4-olide; C005235:methyl ricinoleate; D015094:3-Hydroxyacyl CoA Dehydrogenases; D010100:Oxygen