Mechanistic divergence of two closely related aldol-like enzyme-catalysed reactions.
The analysis of the interaction of threose 4-phosphate and 2-deoxyerythrose 4-phosphate with 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAH7PS) reveals previously unrecognised mechanistic differences between the DAH7PS-catalysed reaction and that catalysed by the closely related enzyme, 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS).
Ahn
Meekyung
M
Institute of Fundamental Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand.
Pietersma
Amy L
AL
Schofield
Linley R
LR
Parker
Emily J
EJ
D010723:3-Deoxy-7-Phosphoheptulonate Synthase; D000446:Aldehyde-Lyases / Q000737:chemistry / Q000378:metabolism*; D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002384:Catalysis; D007700:Kinetics; D015394:Molecular Structure; D009345:Neisseria meningitidis / Q000201:enzymology
D000447:Aldehydes; C116609:3-hydroxybutanal; D010723:3-Deoxy-7-Phosphoheptulonate Synthase; C020011:2-dehydro-3-deoxyphosphooctonate aldolase; D000446:Aldehyde-Lyases