title: Multiplex PCR for simultaneous detection of five virulence hemolysin genes in Vibrio anguillarum.
abstract: A multiplex PCR was developed for detection of hemolysin-producing Vibrio anguillarum using primers targeting five hemolysin genes (vah1, vah2, vah3, vah4 and vah5). This method was successful in amplifying reactions containing as little as 100 fg of genomic template DNA. The direct detection of V. anguillarum in clinical specimens by this multiplex PCR was also successful in reactions containing as few as 10 bacterial cells. This multiplex PCR method can be a rapid and sensitive method for detecting pathogenic V. anguillarum.
authors.affiliation: Laboratory of Genome Science, Graduate school of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan, Japan.
authors.forename: Channarong
authors.initials: C
authors.lastname: Rodkhum
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.forename: Jorge H
authors.initials: JH
authors.lastname: Crosa
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes / Q000382:microbiology; D006460:Hemolysin Proteins / Q000235:genetics*; D006801:Humans; D017684:Oncorhynchus kisutch / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014733:Vibrio / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D004269:DNA, Bacterial; D006460:Hemolysin Proteins