title: [Effects of TGFbeta1 on the transcriptional activity of SP1, AP1 and Smad3-Smad4 in lung fibroblasts].
abstract: OBJECTIVE
  To investigate the effects of TGFbeta1 on the transcriptive activity of SP1, AP1 and Smad3-Smad4 in rat lung fibroblasts(RLF).
  
  
  METHODS
  RLFs were isolated and cultured and then treated with 10 microg/L TGFbeta1. The transcriptive activity of SP1, AP1 and Smad3-Smad4 and the expression of plasminogen activator inhibitor I(PAI-1) and collagen I were detected by means of electrophoretic mobility shift assay (EMSA), Western blot and immunohistochemical staining.
  
  
  RESULTS
  After treatment with 10 microg/L TGFbeta1, AP1, SP1 and Smad3-Smad4 were activated and the expression of collagen I and PAI-1 were increased.
  
  
  CONCLUSIONS
  TGFbeta1 may activate SP1, AP1 and Smad3-Smad4 and thus increase the expression of PAI-1 and collagen I.
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China. leeyoung33@vip.sina.com
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: De-wen
authors.initials: DW
authors.lastname: Wang
authors.forename: Liang-wen
authors.initials: LW
authors.lastname: Song
authors.forename: Rui-yun
authors.initials: RY
authors.lastname: Peng
authors.forename: Ya-bing
authors.initials: YB
authors.lastname: Gao
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Ma
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D024202:Electrophoretic Mobility Shift Assay; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology*; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar; D051900:Smad3 Protein / Q000235:genetics*; D051901:Smad4 Protein / Q000235:genetics*; D016329:Sp1 Transcription Factor / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D051900:Smad3 Protein; C497787:Smad3 protein, rat; D051901:Smad4 Protein; C490811:Smad4 protein, rat; D016329:Sp1 Transcription Factor; D016212:Transforming Growth Factor beta