Quantitative determination of incarvillateine in Incarvillea sinensis by solid phase extraction and high performance liquid chromatography.
Method for rapid quantitative analysis of incarvillateine in Incarvillea sinensis by high-performance liquid chromatography (HPLC) has been developed. The sample preparation involves solid phase extraction (SPE) with a mixed-mode reversed-phase and cation-exchange cartridge. The linear calibration range for incarvillateine was 0.002-0.5 mg/ml. The limit of detection was 0.35 microg/ml (S/N=3). Intra- and interday precisions were less than 0.36% (n=6) and 1.61% (n=18), respectively. The recovery of incarvillateine was 97.61-102.44% with the relative standard deviation (RSD) ranging from 0.63 to 1.93% (n=3). This method was proposed as a simple, rapid and accurate method for quantitative determination of incarvillateine content in various samples of Incarvillea sinensis collected from different areas of China.
Chi
Yu-Ming
YM
Seiwa Pharmaceutical, Ltd., Ibaraki, Japan. chi-59@unimatec.co.jp
Zhao
Xi-Ying
XY
Yoshizawa
Toyokichi
T
Nakamura
Motoyuki
M
Yan
Wen-Mei
WM
Hashimoto
Fumio
F
Chi
Yu-Cheng
YC
Kinjo
Junei
J
Nohara
Toshihiro
T
D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D029402:Bignoniaceae / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D039821:Monoterpenes / Q000737:chemistry* / Q000302:isolation & purification; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013329:Structure-Activity Relationship
D000470:Alkaloids; D039821:Monoterpenes; D012996:Solutions; C400141:incarvillateine