[Enhanced prokaryotic expression of a recombinant Lagurus lagurus zona pellucida subunit 3 with codon optimization].
OBJECTIVE
To improve prokaryotic expression level of Lagurus lagurus zona pellucida 3(LZP3) gene.


METHODS
The rare codons of LZP3 gene fragment were turned into the most frequently used codons in E. coli by overlap PCR. LZP3 gene and mutated LZP3 (LZP3m) gene were cloned into expression vector pGEX4T-1 respectively, and transformed into E. coli BL21(DE3).


RESULTS
The expression level of LZP3m was notably higher than that of LZP3.


CONCLUSIONS
The expression of LZP3 gene in E. coli could be successfully improved by using codon optimization.
Li
Yi-jie
YJ
Key Laboratory of Molecular Biology, College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China. jaylixj@hotmail.com
Zhang
Fu-chun
FC
Zhang
Yu
Y
Wang
Yan-bing
YB
D000818:Animals; D003411:Arvicolinae; D015153:Blotting, Western; D003062:Codon / Q000235:genetics*; D004527:Egg Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics*; D015044:Zona Pellucida / Q000378:metabolism*
D003062:Codon; D004527:Egg Proteins