Promoting protein, a silkworm hemolymph protein promoting in vitro replication of nucleopolyhedrovirus, binds to beta-glucans.
A protein which bound to 125I-labeled peptidoglycan (PGN) was isolated from hemolymph of silkworm larvae. The N-terminal amino acid sequence and the molecular weight of the protein were in accord with those described for Promoting Protein (PP) from the silkworm. The binding of the protein to [125I]PGN was competitively inhibited by various beta-glucans. The binding kinetics of PGN and chitin to the protein were analyzed in a biosensor.
Miyake
Seiji
S
Department of Biochemistry and Biotechnology, Faculty of Agriculture, Tottori University, Japan.
Yamano
Yoshiaki
Y
Morishima
Isao
I
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism / Q000502:physiology*; D012831:Bombyx / Q000737:chemistry*; D006458:Hemolymph / Q000737:chemistry*; D019476:Insect Proteins / Q000378:metabolism / Q000502:physiology; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D014779:Virus Replication; D047071:beta-Glucans / Q000378:metabolism*
D001798:Blood Proteins; D019476:Insect Proteins; D047071:beta-Glucans