High-performance liquid chromatographic determination of vertilmicin in rat plasma using sensitive fluorometric derivatization.
A sensitive and reliable high-performance liquid chromatographic method was developed for the determination of vertilmicin in rat plasma. Derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) followed by C(18) reversed-phase chromatography allowed the fluorimetric detection of vertilmicin. Optimal conditions for the derivatization of vertilmicin are described. The limit of quantification was 0.02 mg/L. The pharmacokinetics of vertilmicin was studied in 24 rats following intramuscular injection (i.m.) of different doses (4, 8, 16, 32 mg/kg of body weight). The pharmacokinetic parameter values were estimated by use of 3P97 program. In this study, we assessed the dose proportionality of vertilmicin after single intramuscular injection doses and obtained new information on the pharmacokinetics of the compound.
Liu
Zhen
Z
Department of Pharmaceutical Analysis, School of Pharmacy, Medical Center of Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, PR China.
Sha
Yunfei
Y
Huang
Taomin
T
Yang
Bei
B
Duan
Geng-Li
GL
D000617:Aminoglycosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005449:Fluorenes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D051381:Rats; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000617:Aminoglycosides; D005449:Fluorenes; C487733:vertilmicin; C054007:1-(9-fluorenyl)methyl chloroformate