The effect of platelet activating factor antagonist BN 52021 on bacterial translocation and ICAM-I expression in experimental obstructive jaundice.
Expression of intracellular adhesion molecule-1 (ICAM-1) in an obstructive jaundice model and the potential protective role of platelet activating factor antagonist over small intestine and liver together with its effects on bacterial translocation are examined in this study. Forty-eight male Wistar albino rats were assigned into four equal groups of 12. In groups I and II, animals were sham operated. In groups III and IV, common bile duct ligation and division were performed. In group I and group III, 0.5 ml/day normal saline was applied intraperitoneally daily from day 2 to 6 of the study; in group II and group IV, 1 mg/kg/day BN 52021 was applied intraperitoneally daily from day 2 to 6 of the study. All animals were sacrificed on postoperative day 7. ICAM-1 expression (CD54 positivity) was analyzed in the liver and ileum tissue by immunohistochemical method. Samples from blood, liver mesenteric lymph nodes, and spleen were cultured under aerobic conditions. It is revealed that ICAM-1 expression was statistically higher in group III, with highest bacterial translocation and liver and spleen injury when compared to other groups. Serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), gamma-glutamyltranspeptidase (GGT), bilirubin, tumor necrosis factor alpha (TNFalpha), and interleukin 1beta(IL-1beta) values were at the highest level in group III, and there was a statistical decrease in group IV compared to group III. The administration of BN52021 in experimental obstructive jaundice is a useful way to reduce liver and intestinal mucosal villi damage by inhibiting bacterial translocation and systemic inflammatory response.
Akyürek
Nusret
N
Department of General Surgery, Gazi University School of Medicine, Ankara, Turkey.
Salman
Bülent
B
Irkörücü
Oktay
O
Tezcaner
Tugan
T
Azili
Cem
C
Erdem
Ozlem
O
Akca
Gülçin
G
Akin
Okan
O
Tatlicioglu
Ertan
E
D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D046934:Ginkgolides; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D007413:Intestinal Mucosa / Q000473:pathology; D041781:Jaundice, Obstructive / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D007783:Lactones / Q000494:pharmacology*; D008099:Liver / Q000473:pathology; D008297:Male; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; D018799:Intercellular Adhesion Molecule-1; C045856:ginkgolide B