title: A silica-based monolithic column in capillary HPLC and CEC coupled with ESI-MS or electrospray-atmospheric-pressure laser ionization-MS.
abstract: We describe the successful coupling of CEC and capillary HPLC with the recently developed atmospheric-pressure laser ionization (APLI) method. APLI is suitable for selectively and sensitively ionizing nonpolar aromatic compounds at ambient pressure for subsequent mass-selective detection. The polycyclic aromatic hydrocarbons used as analytes are first separated either by CEC on a silica-based monolithic column or by capillary HPLC. The eluent, along with a sheath flow, is volatilized by microelectrospray and then selectively ionized by excimer laser (KrF*) radiation via two-photon excitation. A QTOF-MS is used as mass-selective detector. This interface combination makes soft ionization of thermally labile nonpolar aromatic analytes possible.
authors.affiliation: Department of Analytical Chemistry, University of Wuppertal, Wuppertal, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Droste
authors.forename: Marc
authors.initials: M
authors.lastname: Schellenträger
authors.forename: Marc
authors.initials: M
authors.lastname: Constapel
authors.forename: Siegmar
authors.initials: S
authors.lastname: Gäb
authors.forename: Matthias
authors.initials: M
authors.lastname: Lorenz
authors.forename: Klaus J
authors.initials: KJ
authors.lastname: Brockmann
authors.forename: Thorsten
authors.initials: T
authors.lastname: Benter
authors.forename: Dieter
authors.initials: D
authors.lastname: Lubda
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: Schmitz
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification*; D012822:Silicon Dioxide; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D012822:Silicon Dioxide