Mesenchymal stem cells from human bone marrow and adipose tissue: isolation, characterization, and differentiation potentialities.
Comparative study of cultured human bone marrow and adipose tissue (lipoaspirate) mesenchymal stem cells was carried out. The main morphological parameters, proliferative activity, expression of surface and intracellular markers of these cells were characterized. Flow cytofluorometry and histological staining showed that both cell types exhibited similar expression of CD105, CD54, CD106, HLA-I markers, were positively stained for vimentin, ASMA, collagen-1, and fibronectin, but not HLA-DR, CD117, and hemopoietic cell markers. The cells underwent differentiation into adipocytes and osteoblasts under appropriate conditions of culturing. Incubation under neuroinductive conditions led to the appearance of a cell population positively stained for type III beta-tubulin (neuronal differentiation marker).
Romanov
Yu A
YA
Research and Practical Laboratory of Human Stem Cells, National Complex of Cardiology Research and Practice, Ministry of Health of the Russian Federation, Moscow. romanov@cardio.ru
Darevskaya
A N
AN
Merzlikina
N V
NV
Buravkova
L B
LB
D000273:Adipose Tissue / Q000166:cytology*; D015415:Biomarkers / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D014404:Tubulin
D015415:Biomarkers; D014404:Tubulin