Synthesis of novel piperidinyl linker based energy transfer terminators and their potential use in DNA sequencing.
Synthesis of novel piperidinyl linker based ET cassettes and terminators is described These novel terminators are evaluated in the DNA sequencing experiments using thermostable DNA polymerase.
Rao
T Sudhakar
TS
G.E. Healthcare, 800 Centennial Ave., Piscataway, NJ 08855, USA. sudhakar.takellapati@ge.com
Nampalli
Satyam
S
Zhang
Weihong
W
Xiao
Haiguang
H
Kumar
Shiv
S
D004247:DNA / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000494:pharmacology; D006358:Hot Temperature; D007202:Indicators and Reagents / Q000494:pharmacology; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D010880:Piperidines / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010880:Piperidines; C032727:piperidine; D004247:DNA; D004259:DNA-Directed DNA Polymerase