title: [Survivin antisense oligonucleotide induces human Hep-2 cell apoptosis].
abstract: OBJECTIVE
  Survivin highly overexpresses in the most of human tumors, and it may play an important role in the development of tumor. The aim of this study was to explore the effects of survivin antisense oligonucleotide (ASODN) on the proliferation and the apoptosis of human Hep-2 cell.
  
  
  METHODS
  Hep-2 cells were transfected with survivin ASODN mediated by lipofectamine, MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide] method was used to observe the cell growth inhibitory rate, the expressions of survivin mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot respectively. Flow cytometry was used to examine cell apoptosis rate. Kinase activity test was used to detect the changes of caspase-3 activity.
  
  
  RESULTS
  Survivin ASODN obviously inhibited the cell growth of Hep-2 cells after transfection. After transfected with survivin ASODN the expressions of survivin mRNA and protein of Hep-2 cells were down-regulated, and apoptosis rate was significantly increased. The activity of caspase-3 increased highly in Hep-2 cells transfected with survivin ASODN, which showed time-dependent.
  
  
  CONCLUSIONS
  Survivin ASODN could inhibit the proliferation of Hep-2 cell and induced apoptosis through down-regulating the the expressions of survivin mRNA and protein.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Hospital of Tongji Medical college, Huazhong University of Science and Technology, Wuhan 430030, China. yangliu616@yahoo.com.cn
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Lin-lin
authors.initials: LL
authors.lastname: Yu
authors.forename: Yong-hua
authors.initials: YH
authors.lastname: Cui
authors.forename: Yu-xin
authors.initials: YX
authors.lastname: Ji
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D000077022:Survivin
chemical_list: D051017:Apoptosis Regulatory Proteins; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D000077022:Survivin; C505407:CASP3 protein, human; D053148:Caspase 3