title: [Potential antiviral drug Pueraria crude extract and puerarin protect against ethanol-induced cytotoxicity in embryonic mouse hippocampal cultures].
abstract: OBJECTIVE
  To examine whether Chinese medical herb Pueraria crude extract (CP) and standard of pure puerarin (SP) possess the same neuroprotective effects during concomitant ethanol (EtOH) treatment.
  
  
  METHODS
  Hippocampus cultures were prepared from mice at gestational age of 18 day. Cell viability was measured by MTT assay. RT-PCR was employed to determine mRNA expression of superoxide dismutase (SOD).
  
  
  RESULTS
  As measured by MTT assay, supplementation with 15 mg/L CP or 10 mg/L SP afforded neuroprotection against all EtOH concentrations (50, 200 and 350 mmol/L, respectively) in embryonic hippocampal culture system. In addition, both 15 mg/L CP and 10 mg/L SP could decrease expression of SOD at mRNA level.
  
  
  CONCLUSIONS
  This study suggests that CP and SP could decrease oxidative stress induced by ethanol treatment by the decreased expression of SOD at mRNA level, and demonstrates antioxidative neuroprotective effect of CP and SP against developmental ethanol exposure in vitro.
authors.affiliation: Huaxi School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Han
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Li
authors.forename: Zeng-li
authors.initials: ZL
authors.lastname: Yu
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: De-sheng
authors.initials: DS
authors.lastname: Wu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010936:Plant Extracts / Q000494:pharmacology; D011247:Pregnancy; D029908:Pueraria / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics
chemical_list: D000998:Antiviral Agents; D007529:Isoflavones; D010936:Plant Extracts; D012333:RNA, Messenger; D000431:Ethanol; D013482:Superoxide Dismutase; C033607:puerarin