Synaptic physiology: illuminating the road ahead.
A number of genetically encoded reporters of neuronal activity are being developed to assay synaptic activity with single synapse resolution. A recently engineered probe allows imaging of glutamatergic transmission with high sensitivity, and similar probes may help pave the way for optical imaging of excitatory synaptic function in vivo.
Burrone
Juan
J
MRC Centre for Developmental Neurobiology, King's College London, UK. jburrone@kcl.ac.uk
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D003952:Diagnostic Imaging; D004330:Drosophila; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D018698:Glutamic Acid / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D015336:Molecular Probe Techniques; D009469:Neuromuscular Junction / Q000502:physiology / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology
D002118:Calcium; D002135:Calcium-Binding Proteins; D018698:Glutamic Acid; D049452:Green Fluorescent Proteins; D011993:Recombinant Fusion Proteins; C503160:VC6.1 cameleon protein, recombinant