title: Differential detection of S100A8 in transitional cell carcinoma of the bladder by pair wise tissue proteomic and immunohistochemical analysis.
abstract: The search for novel molecular markers of tumor invasion is vital if strategies are to become more effective in the diagnostic and prognostic management of transitional cell carcinoma of the bladder. Up to 50% of tumors detected at stage 1 (pT1) progress to a higher grade even after endoscopic surgical resection, and there are currently no protein markers of this aggressive, invasive phenotype. We have combined SELDI-TOF-MS, ClinProt magnetic bead enrichment, Nano-LC-ESI-ion trap tandem mass spectrometry and immunohistochemical analysis to the study of 12 invasive bladder cancer tissue biopsies paired with normal bladder tissue samples obtained from the same patients for the definition and identification of proteins up-regulated in the tumors. We report the inflammation-associated calcium binding protein S100A8 (MRP-8, calgranulin A) to be highly expressed in tumor cells in contrast to normal urothelium in 50% of the samples, as well as two unidentified protein markers at 5.75 and 6.89 kDa that were differentially detected in 9/12 and 10/12 tumor samples, respectively. These new markers, when fully characterized, may contribute to new target proteins for the prediction of aggressive, invasive bladder tumors.
authors.affiliation: Section for Transplantation Immunology and Immunohematology, University of Tuebingen, Tuebingen, Germany. thomas.flad@uni-tuebingen.de
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Tolson
authors.forename: Thomas
authors.initials: T
authors.lastname: Flad
authors.forename: Volker
authors.initials: V
authors.lastname: Gnau
authors.forename: Hassan
authors.initials: H
authors.lastname: Dihazi
authors.forename: Joerg
authors.initials: J
authors.lastname: Hennenlotter
authors.forename: Alexander
authors.initials: A
authors.lastname: Beck
authors.forename: Gerhard A
authors.initials: GA
authors.lastname: Mueller
authors.forename: Markus
authors.initials: M
authors.lastname: Kuczyk
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Mueller
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D040501:Calgranulin A / Q000378:metabolism*; D002295:Carcinoma, Transitional Cell / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007124:Immunoenzyme Techniques; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D001743:Urinary Bladder / Q000378:metabolism / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D040501:Calgranulin A