title: GABA(A) receptor gamma subunits in the hippocampus of the rat after perforant pathway lesion.
abstract: Immunohistochemical and Western blotting techniques were employed to examine the alterations in immunostaining of the gamma-amino butyric acid (GABA) receptor subunits gamma 1/3 and 2 within the hippocampus of the rat brain at 1, 3, 7, 14, and 30 days after a unilateral perforant pathway lesion. At 1, 3, and 7 days post-lesion, we observed a remarkable decrease in gamma 1/3 neuropil staining in the deafferented zone (i.e., the outer molecular layer of the dentate gyrus ipsilateral to the lesion), although at 3 and 7 days post-lesion, staining intensity was considerably recovered. At 14 days post-lesion, the gamma 1/3 immunostaining was indistinguishable from that of controls and it appeared yet more robust at 30 days post-lesion. We also observed a slight decrease in gamma 2 neuropil staining until 7 days post-lesion, and an increase in gamma 2 staining at 30 days post-lesion. Western blot analysis demonstrated data that was relatively consistent with our immunohistochemical observations, although gamma 3 was hardly detectable. Our study suggests that gamma subunits of the GABA(A) receptor in the dentate gyrus display a plastic response to the deafferentation of the perforant pathway.
authors.affiliation: Department of Psychiatry, Institute of Clinical Medicine, University of Tsukuba, Tsukuba City, Ibaraki, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Iwakiri
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Mizukami
authors.forename: Masanori
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Asada
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D001927:Brain Diseases / Q000378:metabolism; D007839:Functional Laterality; D005786:Gene Expression Regulation / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D019580:Perforant Pathway / Q000293:injuries* / Q000502:physiology; D021122:Protein Subunits / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism*; D013997:Time Factors
chemical_list: D021122:Protein Subunits; D011963:Receptors, GABA-A