title: Identification of a positive Cis-element upstream of human NKX3.1 gene.
abstract: NKX3.1 is a prostate-specific homeobox gene related to prostate development and prostate cancer. In this work, we aimed to identify precisely the functional cis-element in the 197 bp region (from -1032 to -836 bp) of the NKX3.1 promoter (from -1032 to +8 bp), which was previously identified to present positive regulatory activity on NKX3.1 expression, by deletion mutagenesis analysis and electrophoretic mobility shift assay (EMSA). A 16 bp positive cis-element located between -920 and -905 bp upstream of the NKX3.1 gene was identified by deletion mutation analysis and proved to be a functional positive cis-element by EMSA. It will be important to further study the functions and regulatory mechanisms of this positive cis-element in NKX3.1 gene expression.
authors.affiliation: Department of Biochemistry, Medical School of Shandong University, Jinan 250012, China.
authors.forename: An-Li
authors.initials: AL
authors.lastname: Jiang
authors.forename: Peng-Ju
authors.initials: PJ
authors.lastname: Zhang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Hu
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Kong
authors.forename: Zhi-Fang
authors.initials: ZF
authors.lastname: Liu
authors.forename: Hui-Qing
authors.initials: HQ
authors.lastname: Yuan
authors.forename: Jian-Ye
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002874:Chromosome Mapping / Q000379:methods*; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011471:Prostatic Neoplasms / Q000235:genetics*; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D018398:Homeodomain Proteins; C491178:NKX3-1 protein, human; D014157:Transcription Factors