[The role of mitochondrial damages in Helicobacter pylori-induced apoptosis of gastric cancer cells].
OBJECTIVE
To explain the role of mitochondrial pathway in the apoptosis of SGC-7901 cell line induced by concentrated Helicobacter pylori culture supernatant (CHCS).


METHODS
Cytochrome oxidase (COX) I expression was detected by Western blotting. Cell apoptosis and mitochondrial membrane potential were measured by flow cytometry.


RESULTS
CHCS could induce the apoptosis of SGC-7901 in a dose- and time-dependent manner. Apoptotic rates gradually enhanced followed by the concentrations increasing. The mitochondrial membrane potential (MMP) began to descend after treating CHCS for 4 h, and MMP descended most distinctly in 8 h. It descended the lowest point in 12 h, and it had no special changes in 24 h. The expression of COX I was notably lower than that of control group after CHCS treating (632.8 +/- 40.6 vs 895.1 +/- 44.2, P < 0.05).


CONCLUSIONS
Mitochondrial pathway may play an important role in the apoptosis of SGC-7901 cells induced by CHCS.
Lan
Chun-hui
CH
Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Fang
Dian-chun
DC
Fan
Li-lin
LL
He
Zhi-gao
ZG
Chen
Dong-feng
DF
Liu
Chong-yang
CY
Shi
Hong-tao
HT
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D003576:Electron Transport Complex IV / Q000378:metabolism; D005434:Flow Cytometry; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000473:pathology
D003576:Electron Transport Complex IV