Identification of mouse AAV capsid-specific CD8+ T cell epitopes.
Adeno-associated virus has been developed for use as a gene transfer vector. To understand the impact of AAV capsid-specific CD8(+) T cells on AAV-mediated gene transfer, we identified CD8(+) T cell epitopes for AAV-2 and AAV-8 capsid in C57BL/6 (H-2(b) MHC haplotype) and BALB/c (H-2(d) MHC haplotype) mice. Mice of both the H-2(b) and the H-2(d) haplotypes recognized epitopes on AAV-2 and AAV-8 capsid. T cells from H-2(b) mice recognized an epitope that was conserved between AAV-2 and AAV-8 capsid. Cross-reactivity of AAV-specific CD8(+) T cells induced by different AAV serotypes may have important implications for gene transfer. Identification of these epitopes will facilitate studies of immune response to AAV capsid in mouse models.
Sabatino
Denise E
DE
The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
Mingozzi
Federico
F
Hui
Daniel J
DJ
Chen
Haifeng
H
Colosi
Peter
P
Ertl
Hildegund C J
HC
High
Katherine A
KA
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D036022:Capsid Proteins / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics*; D000939:Epitopes / Q000737:chemistry; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry*; D018014:Gene Transfer Techniques; D005821:Genetic Techniques; D015316:Genetic Therapy / Q000379:methods*; D006239:Haplotypes; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D013045:Species Specificity
D036022:Capsid Proteins; D016207:Cytokines; D000939:Epitopes; D018984:Epitopes, T-Lymphocyte; D019151:Peptide Library; D010455:Peptides