[Effect of CCR5delta32, CCR5m303, CCR2-64I and SDF1-3'A gene polymorphism on the prognosis of Chinese HIV-1 carriers].
OBJECTIVE
To study how CCR5delta32, CCR5m303, CCR2-64I, SDF1-3'A gene polymorphisms affect the prognosis of Chinese HIV-1 carrier.


METHODS
Epidemiologic survey was done to the HIV-1 carriers who were found in Shenzhen area. PCR/RFLP technology was applied to analyze CCR5delta32, CCR5m303, CCR2-64I, SDF1-3'A gene polymorphisms of the HIV-1 carriers. The plasma virus load and CD4+ cell counting was assayed. The incubation period of some carriers was estimated. SPSS11.0 software was used to analyze the data.


RESULTS
No persons with CCR5delta32 and CCR5m303 mutation genotype were found from 189 HIV-1 carriers. SDF1-3'A allele frequency was 26.14% and CCR2-64I allele 19.82%. The carriers were divided into high virus load group (virus load < 20,000 copies/ml) and low virus load group (virus load > or =20,000 copies/ml). It was found by one-way ANOVA analysis on the logarithm of virus load that there was no significant difference between CCR2-64I wild genotype and cross bred genotype (P=0.272). One-way ANOVA analysis on delitescence of some carriers showed that there was not significant difference between CCR2-64I wild genotype and cross bred genotype (P=0.662). One-way ANOVA analysis on the logarithm of virus load showed that there was significant difference among SDF1-3'A wild genotype, cross bred genotype and pure mutation genotype (P=0.001).


CONCLUSIONS
CCR2-64I gene mutation may not significantly affect virus load of Chinese HIV-1 carriers, nor it affect the incubation period of HIV-1 carriers. SDF1-3'A gene mutation can decrease virus load, but it may not prolong the incubation period of HIV-1 carriers.
Wang
Xiao-hui
XH
Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China. wxhszcn@sohu.com
Feng
Tie-jian
TJ
Wang
Fu-sheng
FS
Chen
Lin
L
Shi
Xiang-dong
XD
Jin
Lei
L
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000473:pathology* / Q000821:virology; D002353:Carrier State; D054377:Chemokine CXCL12 / Q000235:genetics*; D002681:China; D005787:Gene Frequency; D005838:Genotype; D015497:HIV-1; D006579:Heterozygote; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D019713:Receptors, CCR5 / Q000235:genetics*
D054377:Chemokine CXCL12; D019713:Receptors, CCR5