Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11.
Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with an scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01.
Cheng
Man
M
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Chan
Ceci W L
CW
Cheung
Randy C F
RC
Bikkavilli
Rama Kamesh
RK
Zhao
Qi
Q
Au
Shannon W N
SW
Chan
Paul K S
PK
Lee
Susanna S T
SS
Cheng
Gregory
G
Ho
Walter K K
WK
Cheung
Wing-Tai
WT
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D003429:Cross Reactions / Q000276:immunology; D017370:Interleukin-11 / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000276:immunology*; D051381:Rats; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry / Q000276:immunology*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D000914:Antibodies, Viral; D017370:Interleukin-11; D019590:Nucleocapsid Proteins