Mercury distribution in fish organs and food regimes: Significant relationships from twelve species collected in French Guiana (Amazonian basin).
Within a multidisciplinary research programme set up in French Guiana (Amazonian basin), twelve fish species from six food regimes were collected from the upper part of the Maroni River in order to analyze mercury (Hg) distribution in six organs (gills, liver, kidneys, skeletal muscle, stomach, and intestine) and to look for a relationship between Hg organotropism and food regimes. As many studies have shown, mercury biomagnification leads to extremely marked differences in muscle accumulation levels: the average ratio between extreme concentrations measured in piscivorous and herbivorous species was almost 500. A first principal component analysis on primary Hg concentration variables showed that biomagnification had a marked effect, masking differences between Hg distribution in the organs according to fish species and their food regimes. In order to avoid this, we determined ratios between Hg concentrations measured in the different organs and in the skeletal muscle, considered as the reference tissue for biomagnification effects. A new principal component analysis using these normalized values, in conjunction with a Ward's hierarchical clustering method, revealed that there is a link between Hg organotropism and the food regimes, with comparatively high [Hg]gills/[Hg]muscle ratios for the herbivorous species; high [Hg]intestine-liver-kidneys/[Hg]muscle ratios for the benthivorous and periphytophagous species, and, in contrast, ratios of less than 1 in the different organs for the piscivorous and omnivorous species. Our determinations of methylmercury (MMHg) percentages in the food consumed by the fish (aquatic macrophytes, terrestrial material from the river banks, biofilms, benthic invertebrates, fish muscle tissues), according to the different food regimes (herbivorous, periphytophagous, benthivorous, omnivorous, carnivorous, piscivorous), showed that this criterion can account for the differences in Hg distribution in the fish organs. For instance, the periphytophagous and benthivorous fish species ingest biofilms and small benthic invertebrates with quite low MMHg burdens (18% and 35 to 52% of Hgtotal, respectively). The highest [Hg]organs/[Hg]muscle ratios were observed for the liver and kidneys, the two principal target organs for inorganic Hg in fish. On the other hand, the piscivorous species ingest a large amount of fish of varying size, with high MMHg percentages in their muscle tissue (nearly 80%); Hg organotropism is characterized by high MMHg concentrations in the skeletal muscle and comparatively low [Hg]organs/[Hg]muscle ratios.
Régine
Maury-Brachet
MB
Laboratoire d'Ecophysiologie et Ecotoxicologie des Systèmes Aquatiques (LEESA), UMR CNRS 5805, Université Bordeaux 1, Place Dr Peyneau, 33120 Arcachon, France.
Gilles
Durrieu
D
Yannick
Dominique
D
Alain
Boudou
B
D000818:Animals; D004784:Environmental Monitoring; D005399:Fishes / Q000378:metabolism*; D020387:Food Chain; D005506:Food Contamination; D005616:French Guiana; D005880:Gills / Q000737:chemistry; D006046:Gold; D007422:Intestines / Q000737:chemistry; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008628:Mercury / Q000032:analysis / Q000378:metabolism*; D008767:Methylmercury Compounds / Q000032:analysis / Q000378:metabolism*; D008906:Mining; D009132:Muscles / Q000737:chemistry; D045483:Rivers; D013270:Stomach / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
D008767:Methylmercury Compounds; D014874:Water Pollutants, Chemical; D006046:Gold; D008628:Mercury