title: Different properties of four molecular forms of protein kinase CK2 from Saccharomyces cerevisiae.
abstract: CK2 is a pleiotropic constitutively active serine/threonine protein kinase composed of two catalytic alpha- and two regulatory beta-subunits, whose regulation is still not well understood. It seems to play an essential role in regulation of many cellular processes. Four active forms of CK2, composed of alphaalpha'betabeta', alpha(2)betabeta', alpha'(2)betabeta', and a free alpha'-subunit were isolated from wild-type yeast and strains containing a single deletion of the catalytic subunit. Each species exhibits properties typical for CK2, but they differ in substrate specificity and sensitivity to inhibitors. This suggests that each CK2 isomer may regulate different process or may differ in the way of its regulation.
authors.affiliation: Department of Molecular Biology, Environmental Protection Institute, Catholic University of Lublin, Lublin, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Domańska
authors.forename: Rafał
authors.initials: R
authors.lastname: Zieliński
authors.forename: Konrad
authors.initials: K
authors.lastname: Kubiński
authors.forename: Ewa
authors.initials: E
authors.lastname: Sajnaga
authors.forename: Maciej
authors.initials: M
authors.lastname: Masłyk
authors.forename: Maria
authors.initials: M
authors.lastname: Bretner
authors.forename: Ryszard
authors.initials: R
authors.lastname: Szyszka
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001665:Binding Sites; D047390:Casein Kinase II / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002384:Catalysis; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D021122:Protein Subunits / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017384:Sequence Deletion
chemical_list: D007527:Isoenzymes; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D047390:Casein Kinase II