Analysis of phenols in water by high-performance liquid chromatography using coumarin-6-sulfonyl chloride as a fluorogenic precolumn label.
A simple, sensitive and rapid reversed-phase high-performance liquid chromatography (RP-HPLC) method is proposed for the analysis of some environmentally important phenols in water. The use of coumarin-6-sulphonyl chloride (C6SCl) as a fluorescence-labeling reagent has been investigated. The compound reacts with phenols within 20 min under mild conditions (ambient temperature, pH 9.0) to give sulphonates that can be separated by RP-HPLC employing fluorescence detection at lambda(ex) = 360 and lambda(em) = 460 nm. The optimum conditions for fluorescence, derivatization and chromatographic separation have been established and detection limits in the range 0.1-0.9 microg l(-1) were obtained for the studied compounds. The calibration curves were linear for the range 6-200 microg l(-1) for phenol, 3-200 microg l(-1) for 2-chlorophenol, 4-chlorophenol and 2,3,5-trichlorophenol and for the range of 3-100 microg l(-1) for 2,3-dichlorophenol and 3,5-dichlorophenol. The practical applicability of the method to environmental samples was demonstrated by analyzing drinking and industrial water samples spiked with the phenolic compounds.
Suliman
Fakhr Eldin O
FE
Sultan Qaboos University, College of Science, Box 36, Department of Chemistry, Al-Khod 123, Oman. fsuliman@squ.edu.om
Al-Kindi
Suad S
SS
Al-Kindy
Salma M Z
SM
Al-Lawati
Haider A J
HA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014874:Water Pollutants, Chemical / Q000032:analysis*
D005456:Fluorescent Dyes; D010636:Phenols; D000072317:Polychlorinated Dibenzodioxins; D014874:Water Pollutants, Chemical; C092155:1,2,3,4-tetrachlorodibenzodioxin