Sortase A contributes to pneumococcal nasopharyngeal colonization in the chinchilla model.
Sortase A (SrtA) is required to anchor neuraminidase, beta-galactosidase, and possibly other LPXTG motif proteins to the pneumococcal cell surface. We examined the role of SrtA in Streptococcus pneumoniae nasopharyngeal (NP) colonization in the chinchilla model. The srtA mutant colonized the nasopharynx at a significantly lower level than the D39 parent strain during the second and third week of the carriage, and was eliminated from nasopharynx one week earlier than the D39 pneumococci. Our data indicate that SrtA contributes to pneumococcal NP colonization in this animal model.
Chen
Shu
S
Division of Otologic Research, College of Medicine and Public Health, The Ohio State University, Room 4331, Cramblett Hall, 456 W. 10th Avenue, Columbus, OH 43210, USA.
Paterson
Gavin K
GK
Tong
Hua Hua
HH
Mitchell
Timothy J
TJ
DeMaria
Thomas F
TF
D019881:Aminoacyltransferases / Q000235:genetics / Q000502:physiology*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000201:enzymology; D002682:Chinchilla; D003546:Cysteine Endopeptidases; D004195:Disease Models, Animal; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008297:Male; D009154:Mutation; D009305:Nasopharynx / Q000382:microbiology; D010033:Otitis Media / Q000382:microbiology; D010641:Phenotype; D011008:Pneumococcal Infections / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D013997:Time Factors
D001426:Bacterial Proteins; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases