title: Anti-p80 coilin autoantibodies react with a conserved epitope and are associated with anti-DFS70/LEDGF autoantibodies.
abstract: p80 coilin is a nuclear autoantigen that strongly accumulates in Cajal bodies (CB) and is considered a marker for CBs. No clear clinical features have been associated with anti-p80 autoantibodies, and the epitopes recognized by these antibodies are entirely unknown. We determined the epitope specificity for 34 anti-p80 coilin-positive sera from Japanese patients to better understand its clinical significance. Western blotting and immunoprecipitation analysis revealed that the autoepitope of p80 coilin was restricted to a single region encompassing amino acid residues 421-576 from the C-terminus of p80 coilin. Additionally, sera from 30 of 34 (88%) patients with anti-p80 coilin antibody titers were also positive for anti-DFS70/LEDGF antibody. This autoantibody is sometimes found in patients with some allergic diseases but rarely in patients with connective tissue disease (CTD). Other similar characteristics between these two autoantibodies were also noted. In contrast, few anti-p80 coilin positive patients were positive for CTD-marker antibodies, such as anti-Sm, anti-dsDNA, and anti-SS-A. We conclude that the presence of anti-p80 coilin autoantibodies is not associated with any particular clinical syndromes nor is it diagnostic of any conditions.
authors.affiliation: Division of Connective Tissue Disease and Autoimmunity, Department of Dermatology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Goto
authors.forename: Kazumitsu
authors.initials: K
authors.lastname: Sugiura
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Watanabe
authors.forename: Hiroki
authors.initials: H
authors.lastname: Onouchi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tomita
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Muro
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000276:immunology*; D000328:Adult; D001323:Autoantibodies / Q000097:blood / Q000235:genetics / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D003240:Connective Tissue Diseases / Q000175:diagnosis / Q000276:immunology*; D017124:Conserved Sequence; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000276:immunology*; D014157:Transcription Factors / Q000276:immunology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D001323:Autoantibodies; D015415:Biomarkers; D000939:Epitopes; D036341:Intercellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; C504649:PSIP1 protein, human; D014157:Transcription Factors; C119380:lens epithelium-derived growth factor; C068921:p80-coilin