Microcirculation disturbance affects rats with acute severe pancreatitis following lung injury.
OBJECTIVE
To study the effects of microcirculation disturbance (MD) on rats with acute severe pancreatitis (ASP).


METHODS
We developed ASP rat models, and anatomized separately after 1, 3, 5, 7, and 9 h. We took out blood and did hemorrheologic examination and erythrocyte osmotic fragility test, checked up the water content, capillary permeability, and genetic expression of intercellular adhesion molecule-1 (ICAM-1) in lung tissues, examined the apoptosis degree of blood vessel endothelium while we tested related gene expression of Bax and Bcl-2 in lung tissues. We did the same examination in control group.


RESULTS
The viscosity of total blood and plasma, the hematocrit, and the erythrocyte osmotic fragility were all increased. Fibrinogen was decreased. The water content in lung tissues and capillary permeability were increased. Apoptosis degree of blood vessel endothelium was increased too. ICAM-1 genetic expression moved up after 1 h and reached its peak value after 9 h.


CONCLUSIONS
MD plays an important role in ASP following acute lung injury (ALI). The functional damage of blood vessel endothelium, the apoptosis of capillary vessel endothelium, WBC edging-concentration and the increasing of erythrocyte fragility are the main reasons of ALI.
Liu
Xue-Min
XM
Department of Hepatobiliary Surgery, Xi'an Jiaotong University First Hospital, Xi'an 710061, Shaanxi Province, China. a1090224@pub.xaonline.com
Liu
Qing-Guang
QG
Xu
Jun
J
Pan
Cheng-En
CE
D000208:Acute Disease; D000818:Animals; D008833:Microcirculation; D010195:Pancreatitis / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D011652:Pulmonary Circulation; D051381:Rats; D012128:Respiratory Distress Syndrome / Q000150:complications* / Q000473:pathology / Q000503:physiopathology*; D012720:Severity of Illness Index