title: Protein kinase I of Mycobacterium tuberculosis: cellular localization and expression during infection of macrophage-like cells.
abstract: Protein kinase I of Mycobacterium tuberculosis, which has an unusual amino acid composition in its catalytic loop, displayed autophosphorylation and transphosphorylation activity. Immunoblot analysis of sub-cellular fractions of M. tuberculosis, using anti-PknI antibodies raised in rabbits, showed that PknI localizes to the bacterial cytosol. In contrast, PknA was membrane-bound. Relative expression of pknI, when measured by combining molecular beacons and RT-PCR, decreased during infection of THP-1 human macrophages. Expression of pknA and pknB was upregulated during infection. Thus PknI represents a group of protein kinases that is distinct from the more extensively studied enzymes PknA and PknB.
authors.affiliation: Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
authors.forename: Anubha
authors.initials: A
authors.lastname: Singh
authors.forename: Yogendra
authors.initials: Y
authors.lastname: Singh
authors.forename: Richard
authors.initials: R
authors.lastname: Pine
authors.forename: Lanbo
authors.initials: L
authors.lastname: Shi
authors.forename: Ramesh
authors.initials: R
authors.lastname: Chandra
authors.forename: Karl
authors.initials: K
authors.lastname: Drlica
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D008264:Macrophages / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011817:Rabbits; D015533:Transcriptional Activation
chemical_list: D001426:Bacterial Proteins; D011494:Protein Kinases; D051057:Proto-Oncogene Proteins c-akt; D017868:Cyclic AMP-Dependent Protein Kinases