[Cloning, expression and purification of interferon-kappa, a novel human interferon, and its antiviral activity].
OBJECTIVE
To prepare human interferon-k (hIFN-kappa) and study its biological activities.


METHODS
Whole length of hIFN-kappa's cDNA was cloned, and its sequence was chemically synthesized according to the optimized codons of E.coli, then was expressed in E.coli DH5alpha. After purified, the rhIFN-kappa protein was tested for its various kinds of biological activities.


RESULTS
The purity of rhIFN-kappa was above 90%. In WHIS-VSV system, the antiviral activity of rhIFN-kappa was 2.0 x 10(6) IU/mg. Compared with rhIFN-alpha-2b, the biological activities of rhIFN-kappa were all feeble, including antiviral activity, promoting NK cell activity and anti-proliferation activity.


CONCLUSIONS
Antiviral activities of rhIFN-kappa on cell lines of different species are different, different viruses show different sensitivity to rhIFN-kappa.
Zhang
Hui
H
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Beijing 100052, China.
Duan
Zhao-jun
ZJ
Zhu
Jian-gao
JG
Peng
Fu-wang
FW
Li
Wu-ping
WP
Gao
Han-chun
HC
Xie
Zhi-ping
ZP
Wang
Yu-e
YE
Hou
Yun-de
YD
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007370:Interferon Type I / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D014709:Vero Cells
D000998:Antiviral Agents; D018076:DNA, Complementary; D007370:Interferon Type I; D011994:Recombinant Proteins; C439669:interferon kappa