title: The ribostamycin biosynthetic gene cluster in Streptomyces ribosidificus: comparison with butirosin biosynthesis.
abstract: A cluster of genes for ribostamycin (Rbm) biosynthesis was isolated from Streptomyces ribosidificus ATCC 21294. Sequencing of 31.892 kb of the genomic DNA of S. ribosidificus revealed 26 open reading frames (ORFs) encoding putative Rbm biosynthetic genes as well as resistance and other genes. One of ten putative Rbm biosynthetic genes, rbmA, was expressed in S. lividans TK24, and shown to encode 2-deoxy-scyllo-inosose (DOI) synthase. Acetylation of various aminoglycoside-aminocyclitol (AmAcs) by RbmI confirmed it to be an aminoglycoside 3-N-acetyltransferase. Comparison of the genetic control of ribostamycin and butirosin biosynthesis pointed to a common biosynthetic route for these compounds, despite the considerable differences between them in genetic organization.
authors.affiliation: Institute of Biomolecule Reconstruction, Sun Moon University, Asan 336-708, Korea.
authors.forename: Bimala
authors.initials: B
authors.lastname: Subba
authors.forename: Madan Kumar
authors.initials: MK
authors.lastname: Kharel
authors.forename: Hei Chan
authors.initials: HC
authors.lastname: Lee
authors.forename: Kwangkyoung
authors.initials: K
authors.lastname: Liou
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Sohng
mesh_terms: D000107:Acetylation; D000617:Aminoglycosides / Q000378:metabolism; D002076:Butirosin Sulfate / Q000096:biosynthesis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D024881:Drug Resistance, Bacterial / Q000235:genetics; D008954:Models, Biological; D005810:Multigene Family; D012271:Ribostamycin / Q000096:biosynthesis*; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: D000617:Aminoglycosides; D012271:Ribostamycin; D002076:Butirosin Sulfate