DNA self-assembling systems: branched oligonucleotides as building blocks and monitoring by pyrene excimer band formation.
The construction of a DNA self-assembling system created by four Y-shaped branched oligodeoxynucleotide building blocks has been studied. The assembly was verified by changes in the fluorescence emission spectra and revealed an additive effect in pyrene excimer band formation during DNA self-assembly.
Lindegaard
Dorthe
D
Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, Odense M DK-5230, Denmark. dhl@chem.sdu.uk
Babu
B Ravindra
BR
Wengel
Jesper
J
D001483:Base Sequence; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D015195:Drug Design; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D046911:Macromolecular Substances / Q000737:chemistry; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D015342:DNA Probes; D046911:Macromolecular Substances; D009696:Nucleic Acids; D009841:Oligonucleotides; D011721:Pyrenes; D004247:DNA