Measuring the effect of glutamate receptor agonists on extracellular D-serine concentrations in the rat striatum using online microdialysis-capillary electrophoresis.
Online microdialysis-capillary electrophoresis (CE) was used to measure the changes in extracellular D-serine concentration in response to the application of glutamate agonists and antagonists in the rat striatum. The microdialysis-CE assay was capable of measuring concentration changes as small as 8% with a sampling rate of 12-15s. Kainic acid (KA) induced increases in D- and L-serine concentrations. Application of the non-NMDA receptor antagonist CNQX did not affect the increases observed for D- or L-serine, suggesting a nonspecific effect. NMDA also induced increases in D-serine, L-serine, glutamate and GABA concentrations. These increases were attenuated by the NMDA receptor antagonist MK-801.
Ciriacks
Chanda M
CM
University of Minnesota, Department of Chemistry, 207 Pleasant Street SE, Minneapolis, MN 55455, USA.
Bowser
Michael T
MT
D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D019075:Electrophoresis, Capillary; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005110:Extracellular Space / Q000187:drug effects; D018698:Glutamic Acid / Q000378:metabolism; D008297:Male; D017551:Microdialysis / Q000295:instrumentation*; D009862:Online Systems; D051381:Rats; D017207:Rats, Sprague-Dawley; D012694:Serine / Q000378:metabolism*; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D018698:Glutamic Acid; D012694:Serine; D005680:gamma-Aminobutyric Acid