A new approach to oligonucleotide N3'-->P5' phosphoramidate building blocks.
A new synthetic approach to 5-phosphoramidites of 3'-aminonucleosides was developed. The methodology relies upon the use of 3'-amino-2',3'-dideoxy nucleosides as the key starting materials. The final phosphoramidite products were obtained with high yields via 2-3-step efficient chemical transformations using selective introduction of orthogonal protective groups to the 3'-aminonucleoside sugar and base moieties.
Zielinska
Daria
D
Geron Corporation, Menlo Park, California, USA.
Pongracz
Krisztina
K
Gryaznov
Sergei
S
D001482:Base Composition; D006151:Guanosine / Q000737:chemistry; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D009584:Nitrogen / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D010758:Phosphorus / Q000737:chemistry*; D013936:Thymidine / Q000737:chemistry
D009705:Nucleosides; D009841:Oligonucleotides; D006151:Guanosine; D010758:Phosphorus; D009584:Nitrogen; D013936:Thymidine