title: Terminal phosphate labeled nucleotides: synthesis, applications, and linker effect on incorporation by DNA polymerases.
abstract: A number of terminal phosphate-labeled nucleotides with three or more phosphates and with varied length linkers attached between the terminal phosphate and the dye have been synthesized. These nucleotides have been tested as substrates for different DNA and RNA polymerases. We have also explored their utility in DNA sequencing, SNP analysis, nucleic acid amplification, quantitative PCR, and other biochemical assays.
authors.affiliation: GE Healthcare, 800 Centennial Ave., Piscataway, NJ 08855-1327, USA.
authors.forename: Shiv
authors.initials: S
authors.lastname: Kumar
authors.forename: Anup
authors.initials: A
authors.lastname: Sood
authors.forename: Jeffery
authors.initials: J
authors.lastname: Wegener
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Finn
authors.forename: Satyam
authors.initials: S
authors.lastname: Nampalli
authors.forename: John R
authors.initials: JR
authors.lastname: Nelson
authors.forename: Anuradha
authors.initials: A
authors.lastname: Sekher
authors.forename: Paul
authors.initials: P
authors.lastname: Mitsis
authors.forename: John
authors.initials: J
authors.lastname: Macklin
authors.forename: Carl W
authors.initials: CW
authors.lastname: Fuller
mesh_terms: D004396:Coloring Agents / Q000494:pharmacology; D004247:DNA / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D005821:Genetic Techniques; D008956:Models, Chemical; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D017931:DNA Primers; D009711:Nucleotides; D010710:Phosphates; D004247:DNA; D004259:DNA-Directed DNA Polymerase