Meso-dihydroguaiaretic acid from Machilus thunbergii SIEB et ZUCC., and its effects on the expression of matrix metalloproteinase-2, 9 cause by ultraviolet irradiated cultured human keratinocyte cells (HaCaT).
Ethanol and aqueous extracts of Machilus thunbergii used traditionally for the treatments a wide variety of diseases were screened in vitro for the matrix metalloproteinase (MMP)-9 inhibitor actions. Meso-dihydroguaiaretic acid from the stems bark of Machilus thunbergii showed significant MMP-9 inhibition in human keratinocyte cells cause by ultraviolet irradiation. Here we investigated the effect of meso-dihydroguaiaretic acid, which was isolated from Machilus thunbergii, on UV-induced premature skin aging. We studied the effect of meso-dihydroguaiaretic acid on UV-induced MMP-9 expression in an immortalized human keratinocyte cell line, HaCaT, in vitro. Acute UV irradiation induced MMP-9 expression at both the mRNA and protein levels and meso-dihydroguaiaretic acid suppressed this UV-induced MMP-9 expression in a dose-dependent manner. Taken together, these results show that meso-dihydroguaiaretic acid can prevent the harmful effects of UV that lead to skin aging. Therefore, we suggest that meso-dihydroguaiaretic acid should be viewed as a potential therapeutic agent for preventing and/or treating premature skin aging.
Moon
Hyung In
HI
Department of Dermatology, Seoul National University College of Medicine, Korea. himun@snu.ac.kr
Chung
Jin Ho
JH
D002460:Cell Line; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D019770:Lauraceae / Q000737:chemistry*; D017705:Lignans / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D009580:Nitroblue Tetrazolium; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014466:Ultraviolet Rays
D017705:Lignans; D009580:Nitroblue Tetrazolium; C015816:dihydroguaiaretic acid; D012313:RNA; D006139:Guaiacol; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9