Effect of VEGF on tail artery interpositional loop (TAIL) flap: a rodent model for flap prefabrication.
The authors introduce an experimental model of flap prefabrication, the tail artery interpositional loop (TAIL) flap. In this model, an arterial segment from a rat tail is used to create an arteriovenous (A-V) fistula. This fistula is positioned beneath the abdominal skin flap to vascularize the overlying tissue, and a barrier of Silastic sheeting is placed below the fistula to prevent vascular ingrowth from the underlying bed. The efficacy of this new model was tested by investigating the effect of a single topical application of recombinant human VEGF (165). Treatment and control groups each contained 20 animals. In the control group, mean survival skin areas at 1, 2, 3, and 4 weeks were 12.5 percent, 27 percent, 35 percent, and 50 percent, respectively. In the VEGF (165)-treated group, survival rates were 14.8 percent, 37 percent, 48 percent, and 74.3 percent, respectively. Statistically significant differences were noted between the two groups at the 2-week ( p = 0.047), 3-week ( p = 0.048), and 4-week ( p = 0.023) time intervals. The authors conclude that the TAIL flap is a novel and useful animal model to study flap prefabrication.
Stebbins
William G
WG
Division of Plastic and Reconstructive Surgery, Mount Sinai Medical Center, New York, NY 10029, USA.
Fan
Cathy
C
Silver
Lester
L
Chun
Jin K
JK
D000818:Animals; D001158:Arteries / Q000187:drug effects*; D001166:Arteriovenous Shunt, Surgical / Q000379:methods*; D005260:Female; D006085:Graft Survival / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology*; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013524:Surgical Flaps / Q000098:blood supply*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
D006133:Growth Substances; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat