title: Analysis of alkyl polyglucosides in industrial products by capillary electrophoresis with pulsed amperometric detection.
abstract: Pulsed amperometric detection following micellar electrokinetic chromatography has been applied successfully to the direct detection of alkyl polyglucosides (APGs) in shampoos and other industrial products without prior conversion to highly absorbing or fluorescing derivatives. For electrochemical detection, it is necessary to dissociate the hydroxyl groups of the APGs. Thus, we used 0.1 M NaOH in the outlet vial to dissociate the APGs. The main problems associated with the combination of electrochemical detection and capillary electrophoresis are the need to isolate the detector from the electric field used in the capillary electrophoresis separation and the difficulty of aligning the working electrode with the end of the capillary. To overcome these problems, a simple capillary-electrode holder was constructed. This holder automatically aligns the capillary and the electrode in a wall-jet configuration without the aid of micropositioners and facilitates the replacement of electrodes and capillaries without reconstruction of the entire capillary/electrode setup. Special microcylindrical gold electrodes have been produced by sealing 300-microm-diameter gold wire into borosilicate-glass capillaries.
authors.affiliation: Department of Analytical Chemistry, University of Wuppertal, Gauss-Str. 20, 42119, Wuppertal, Germany.
authors.forename: Jane
authors.initials: J
authors.lastname: Hübner
authors.forename: Anh
authors.initials: A
authors.lastname: Nguyen
authors.forename: Florin
authors.initials: F
authors.lastname: Turcu
authors.forename: David
authors.initials: D
authors.lastname: Melchior
authors.forename: Hans-Willi
authors.initials: HW
authors.lastname: Kling
authors.forename: Siegmar
authors.initials: S
authors.lastname: Gäb
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: Schmitz
mesh_terms: D000478:Alkylation; D002236:Carbohydrate Conformation; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry*; D007221:Industry
chemical_list: D005960:Glucosides