title: DNA repair capacity measured by high throughput alkaline comet assays in EBV-transformed cell lines and peripheral blood cells from cancer patients and healthy volunteers.
abstract: We collected peripheral blood (PB) from 556 patients with various types of cancer who had undergone radiotherapy and from 81 healthy volunteers. We exposed whole PB and Epstein-Barr virus-transformed lymphoblastoid cell lines (EBLs) derived from the PB mononucleocytes to X-irradiation (5 Gy). Using the alkaline comet assay, we measured the immediate DNA damage and, at 15 min, the % residual damage. In PB, the immediate damage was similar in patients and healthy volunteers while the % residual damage (mean+/-S.D.) was significantly higher in patients with breast (54.3+/-A23.9), cervical (54.7+/-A23.9), head/neck (56.8+/-A24.4), lung (60.1+/-23.5), or esophageal cancers (59.5+/-A33.7) than in healthy donors (42.9+/-19.6) (P<0.05). We did not observe such differences in the EBV-transformed cell lines. Thus, radiation sensitivity of fresh PB cells measured by the alkaline comet assay was related to cancer status.
authors.affiliation: RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences, 4-9-1, Anagawa, Inage-ku, Chiba 263-8555, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Iwakawa
authors.forename: Miyako
authors.initials: M
authors.lastname: Goto
authors.forename: Shuhei
authors.initials: S
authors.lastname: Noda
authors.forename: Masashi
authors.initials: M
authors.lastname: Sagara
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yamada
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Matsui
authors.forename: Yukimasa
authors.initials: Y
authors.lastname: Miyazawa
authors.forename: Hideya
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsuji
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ohno
authors.forename: Junetsu
authors.initials: J
authors.lastname: Mizoe
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Tsujii
authors.forename: Takashi
authors.initials: T
authors.lastname: Imai
mesh_terms: D001782:Blood Donors; D002461:Cell Line, Transformed / Q000528:radiation effects; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000528:radiation effects*; D009369:Neoplasms / Q000097:blood / Q000235:genetics* / Q000532:radiotherapy; D011836:Radiation Tolerance / Q000235:genetics*