Deoxycholic acid-conjugated chitosan oligosaccharide nanoparticles for efficient gene carrier.
To develop chitosan based efficient gene carriers, highly purified chitosan oligosaccharides (COSs) were chemically modified with deoxycholic acid (DOCA). Owing to the amphiphilic characters, the DOCA-conjugated COSs (COSDs) formed self-aggregated nanoparticles in aqueous milieu. The physicochemical characterization revealed that the particle size of the nanoparticles was in the range of 200 approximately 240 nm and the critical aggregation concentration (cacs) was 0.012 approximately 0.046 g/L, depending on the degree of substitution (DS). As efficient gene carriers, the COSD nanoparticles showed superior gene condensation and protection of condensed gene from endonuclease attack than unmodified COSs. Furthermore, COSDs showed great potential for gene carrier with the high level of gene transfection efficiencies, even in the presence of serum. Considered with the negligible cytotoxic effects, DOCA-modified chitosan oligosaccharides can be considered as potential candidates for efficient non-viral gene carriers.
Chae
Su Young
SY
Department of Polymer Science and Engineering, Sunchon National University, Suncheon, Jeonnam, Korea.
Son
Sohee
S
Lee
Minhyung
M
Jang
Mi-Kyeong
MK
Nah
Jae-Woon
JW
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D003840:Deoxycholic Acid / Q000737:chemistry*; D004337:Drug Carriers; D015316:Genetic Therapy; D006801:Humans; D007202:Indicators and Reagents; D008027:Light; D008970:Molecular Weight; D049329:Nanostructures; D009844:Oligosaccharides / Q000737:chemistry*; D010316:Particle Size; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D014162:Transfection
D004337:Drug Carriers; D007202:Indicators and Reagents; D009844:Oligosaccharides; D003840:Deoxycholic Acid; D004247:DNA; D048271:Chitosan