Isolation and incorporation into lipid vesicles of a concanavalin A receptor from human erythrocytes.
Affinity chromatography has been used to isolate a concanavalin A receptor portion of Band 3 from humen erythrocytes in the presence of the readily-dialysable detergent, dodecyltrimethylammonium bromide. Addition of phospholipids to the isolated fraction and removal of detergent by dialysis leads to formation of vesicles containing the receptor. Intramembranous particles similar in size and shape to those seen in intact erythrocytes are a characteristic of the reconstituted preparations. Vesicles containing receptor bind concanavalin A with high affinity.
Barratt
D G
DG
Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada.
Sharom
F J
FJ
Thede
A E
AE
Grant
C W
CW
D001703:Biophysics / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005614:Freeze Fracturing; D006388:Hemagglutinins / Q000737:chemistry; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D008854:Microscopy, Electron; D017830:Octoxynol / Q000494:pharmacology; D010743:Phospholipids / Q000737:chemistry; D011485:Protein Binding; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D011952:Receptors, Concanavalin A / Q000737:chemistry*
D003902:Detergents; D006388:Hemagglutinins; D008051:Lipid Bilayers; D008055:Lipids; D010743:Phospholipids; D000644:Quaternary Ammonium Compounds; D011952:Receptors, Concanavalin A; D003208:Concanavalin A; D017830:Octoxynol; C022806:dodecyltrimethylammonium