title: Effect of nitric oxide on hyperpolarization-activated current in substantia gelatinosa neurons of rats.
abstract: Central sensitization is the hyperexcitability of spinal processing after peripheral nerve injury or inflammation. This phenomenon may be associated with nitric oxide (NO) signal pathway in synapse. Here, we have investigated the effect of NO on hyperpolarization-activated inward current (I(h)) in substantia gelatinosa (SG) neurons, using the whole-cell patch clamp technique. I(h) was increased by the application of sodium nitro prusside (SNP, a NO donor) or 8Br-cGMP. The stimulatory effects of NO were abolished by guanylyl cyclase inhibitor, ODQ, suggesting that the effect of NO was mediated by cGMP. However, this effect of NO was not prevented by the pretreatment with KT5823, PKG inhibitor. Taken together, the activation of I(h) in SG neurons could be mediated by NO-cGMP dependent pathway. These results reveal an involvement of NO in excitability of SG neuron via the activation of I(h) may be associated with central sensitization.
authors.affiliation: Department of Physiology, Kangwon National University College of Medicine, 192-1 Hyoza 2Dong, Chuncheon 200-701, Republic of Korea.
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Sang Jeong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jun
authors.initials: J
authors.lastname: Kim
authors.forename: Seog Bae
authors.initials: SB
authors.lastname: Oh
authors.forename: Hana
authors.initials: H
authors.lastname: Cho
authors.forename: Sung Jun
authors.initials: SJ
authors.lastname: Jung
mesh_terms: D000818:Animals; D006152:Cyclic GMP / Q000378:metabolism; D017869:Cyclic GMP-Dependent Protein Kinases / Q000378:metabolism; D004553:Electric Conductivity; D004594:Electrophysiology; D006162:Guanylate Cyclase / Q000378:metabolism; D066298:In Vitro Techniques; D009569:Nitric Oxide / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013376:Substantia Gelatinosa / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
chemical_list: D009569:Nitric Oxide; D017869:Cyclic GMP-Dependent Protein Kinases; D006162:Guanylate Cyclase; D006152:Cyclic GMP