Production of deuterated zeaxanthin by Flavobacterium multivorum and its detection by resonance Raman and mass spectrometric methods.
Flavobacterium multivorum, a zeaxanthin-producing organism, was grown aerobically in a medium prepared with deuterated water. Atmospheric pressure chemical ionization mass spectrometry (APCI-MS) and resonance Raman spectroscopy (RRS) analysis revealed approximately 75% replacement of hydrogen by deuterium atoms as indicated by the molecular mass cluster at around m/z 600. Deuterated zeaxanthin upon excitation with a 488 nm laser exhibited characteristic resonance Raman vibrational modes at 1,161 and 1,504 cm(-1) as compared to 1,007, 1,159 and 1,525 cm(-1 )for undeuterated zeaxanthin. HPLC/APCI-MS and HPLC/RRS were specific and sensitive with limits of detection of 2.5 pg and 50 ng, respectively.
Bhosale
Prakash
P
Department of Ophthalmology and Visual Sciences, Moran Eye Center, University of Utah School of Medicine, 50 North Medical Drive, Salt Lake City, UT 84132, USA.
Teredesai
Pallavi V
PV
Lihong
Jin
J
Ermakov
Igor V
IV
Gellermann
Werner
W
Bernstein
Paul S
PS
D017666:Deuterium Oxide / Q000378:metabolism*; D005417:Flavobacterium / Q000737:chemistry / Q000254:growth & development*; D021241:Spectrometry, Mass, Electrospray Ionization; D013059:Spectrum Analysis, Raman; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry
D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D017666:Deuterium Oxide