Effect of dermal exposure to paraphenylenediamine and linear alkylbenzene sulphonate in guinea pigs.
OBJECTIVE
To study the effects of paraphenylenediamine (PPD) and linear alkylbenzene sulphonate (LAS) alone and in combination on the skin.


METHODS
Forty-eight guinea pigs were divided equally into 4 groups and exposed to PPD (4 mg/kg), LAS (12 mg/kg) and PPD (4 mg/kg) plus LAS (12 mg/kg) for 30 days. The biochemical parameters such as acid phosphatase, gtutathione-s-transferase, glutathione peroxidase, glutathione, lipid peroxidation and histamine contents in exposed skin were estimated. The histopathological examination of the exposed skin was also carried out.


RESULTS
The skin enzymes, lipid peroxidation, and histamine increased while glutathione decreased in skin. The simultaneously exposed group showed additive toxic effects. The histopathological examination showed severe hyperkeratosis, thickening of collagen fibres and vacuolisation of epidermal cells in PPD plus LAS exposed skin.


CONCLUSIONS
The findings of the present study suggest that simultaneous exposure to PPD and LAS has additive toxic effects.
Mathur
A K
AK
Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Lucknow, India.
Raizada
R B
RB
Srivastava
M K
MK
Singh
A
A
D000135:Acid Phosphatase / Q000096:biosynthesis; D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D004396:Coloring Agents / Q000633:toxicity*; D004357:Drug Synergism; D005966:Glucuronidase / Q000096:biosynthesis; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000096:biosynthesis; D005982:Glutathione Transferase / Q000096:biosynthesis; D006168:Guinea Pigs; D006632:Histamine / Q000096:biosynthesis; D015227:Lipid Peroxidation; D008297:Male; D010655:Phenylenediamines / Q000633:toxicity*; D012867:Skin / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D013501:Surface-Active Agents / Q000633:toxicity*
D017738:Alkanesulfonic Acids; D004396:Coloring Agents; D010655:Phenylenediamines; D013501:Surface-Active Agents; C030641:alkylbenzyl sulfonic acid; D006632:Histamine; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D000135:Acid Phosphatase; D005966:Glucuronidase; D005978:Glutathione; C029728:4-phenylenediamine