Heat shock protein HSP70 increases the resistance of cortical cells to glutamate excitotoxicity.
Preincubation of cultured slices of the olfactory cortex of rat brain with heat shock protein in a concentration of 1 microg/ml protected the pre- and postsynaptic mechanisms of glutamatergic synaptic transmission from glutamate excitotoxicity (50 mM) inducing blockade of excitatory postsynaptic function and reducing presynaptic processes. It was hypothesized that heat shock protein protects AMPA and NMDA receptor-mediated processes.
Mokrushin
A A
AA
I. P. Pavlov Institute of Physiology, Russian Academy of Sciences; B. P. Konstantinov St. Petersburg Institute of Nuclear Researches, Russian Academy of Sciences, Gatchina. mok@inbox.ru
Pavlinova
L I
LI
Plekhanov
A Yu
AY
D000818:Animals; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000378:metabolism / Q000633:toxicity*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D009498:Neurotoxins / Q000378:metabolism / Q000633:toxicity*; D009833:Olfactory Pathways / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D009435:Synaptic Transmission / Q000187:drug effects*
D018840:HSP70 Heat-Shock Proteins; D009498:Neurotoxins; D018698:Glutamic Acid