title: A persistent pesticide residue and the unusual catalytic proficiency of a dehalogenating enzyme.
abstract: The soil of potato fields in The Netherlands harbors bacteria with the ability to metabolize 3-chloroacrylic acid, generated by the degradation of a pesticide (1,3-dichloropropene) that entered the environment in 1946. From examination of rate constants at elevated temperatures, we infer that the half-time at 25 degrees C for spontaneous hydrolytic dechlorination of trans-3-chloroacrylic acid is 10,000 years, several orders of magnitude longer than half-times for spontaneous decomposition of other environmental pollutants such as 1,2-dichloroethane (72 years), paraoxon (13 months), atrazine (5 months), and aziridine (52 h). With thermodynamic parameters for activation similar to those for the spontaneous hydration of fumarate at pH 6.8, this slow reaction proceeds at a constant rate through the pH range between 2 and 12. However, at the active site of the enzyme 3-chloroacrylate dehalogenase (CaaD), isolated from a pseudomonad growing in these soils, hydrolytic dechlorination proceeds with a half-time of 0.18 s. Neither k(cat) nor k(cat)/K(m) is reduced by increasing solvent viscosity with trehalose, implying that the rate of enzymatic dechlorination is controlled by chemical events in catalysis rather than by diffusion-limited substrate binding or product release. CaaD achieves an approximately 10(12)-fold rate enhancement, matching or surpassing the rate enhancements produced by many enzymes that act on more conventional biological substrates. One of those enzymes is 4-oxalocrotonate tautomerase, with which CaaD seems to share a common evolutionary origin.
authors.affiliation: Department of Biochemistry and Biophysics and Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Horvat
authors.forename: Richard V
authors.initials: RV
authors.lastname: Wolfenden
mesh_terms: D000179:Acrylates / Q000378:metabolism*; D000498:Allyl Compounds / Q000378:metabolism*; D002384:Catalysis; D006843:Hydrocarbons, Chlorinated; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*; D010573:Pesticide Residues / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*; D013816:Thermodynamics
chemical_list: D000179:Acrylates; D000498:Allyl Compounds; D006843:Hydrocarbons, Chlorinated; D010573:Pesticide Residues; C071773:3-chloroacrylic acid; C023891:1,3-dichloro-1-propene; D006867:Hydrolases; C077721:trans-3-chloroacrylic acid dehalogenase