A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA.
We report a broadly applicable approach for the development of small-molecule-based RNA sensors. Our photoinduced electron transfer (PET) sensor consists of a fluorescein derivative as the fluorophore and two aniline derivatives as electron donors (quenchers). The isolation of electron-donor-binding RNA by in vitro selection (also known as SELEX) yielded an RNA aptamer that could increase the fluorescence intensity of the sensor by 13-fold. This result shows that RNA-electron-donor interactions can be used to develop modular RNA chemosensors.
Sparano
Brian A
BA
Department of Chemistry, University of Pittsburgh, Pennsylvania 15260, USA.
Koide
Kazunori
K
D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013869:Thiolester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D005456:Fluorescent Dyes; D012313:RNA; C402226:LYPLA1 protein, human; D013869:Thiolester Hydrolases