[Methodological studies on selectively removing toxins in Aristolochiae manshuriensis by chinese processing techniques].
OBJECTIVE
To investigate the possibility of selectively detoxifying aristolochic acids in Aristolochiae manshuriensis (Guanmutong) by the chemical properties of aristolochic acids and traditional Chinese processing experience.


METHODS
The technical parameters in processing technique of A. manshuriensis were optimized by orthogonal designed methods with aristolochic acid A.


RESULTS
The processing technique was soaked in 0.1 mol x L(-1) baking soda for several times and then processing with vinegar. Temperature was important factor to detoxify aristolochic acids. aristolochic acid A were removed over 90% from A. manshuriensis in laboratory and over 80% in medium-scale production by 10 batches from two origins of botanical drugs with different contents, and decreased to 0.35-0.60 mg x g(-1) in processed products. aristolochic acid A existed mostly salt-forms in the botanical drug.


CONCLUSIONS
Most toxic components in Guanmutong could be removed by the new processing method.
Wang
Zhi-min
ZM
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China. zhmw123@263.net
You
Li-shuang
LS
Jiang
Xu
X
Li
Lin
L
Wang
Wei-hao
WH
Wang
Guan
G
D019342:Acetic Acid; D030022:Aristolochia / Q000737:chemistry*; D034341:Aristolochic Acids / Q000032:analysis*; D006358:Hot Temperature; D019999:Pharmaceutical Solutions / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D017693:Sodium Bicarbonate; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature
D034341:Aristolochic Acids; D019999:Pharmaceutical Solutions; D017693:Sodium Bicarbonate; C000228:aristolochic acid I; D019342:Acetic Acid