Preventive effect of the soluble tumor-associated antigens on DMBA induced tumorigenesis in C3H/He mice.
In our previous studies, we showed that soluble tumor-associated antigens (sTAA) of 66 kDa and 51 kDa have distinct tumor-preventive effects on chemically induced mammary cancer in rats and are able to repair the damage caused by tumorigenesis in its early stages. In the present study, we investigated whether these proteins can prevent the development of chemically induced tumors in mice. The study was performed on C3H/He mice which have the ability to develop many spontaneous tumors with age. Forty-four, 6-week-old mice were exposed twice at a 2-week interval to the carcinogen 9,10-dimethyl-1,2-benz(alpha)anthracene (DMBA), at a dose of 2 mg/mouse administered intragastrically. Two months later, the mice were divided into two groups. One group received sterile saline twice a week at a dose of 0.2 ml/mouse, intraperitoneally (i.p.). The other group received sTAA twice a week at a dose of about 10 microl in 0.2 ml of sterile saline/mouse, i.p. Periodically, all mice were checked for the presence of tumors. The experiment was terminated at week 35. Vaccination with sTAA increased the time of involvement of mice in the experiment, prevented the tumorigenic effect of DMBA, and inhibited further development of existing tumors.
Kossoy
George
G
The Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel.
Ben-Hur
Herzl
H
Elhayany
Asher
A
Schneider
David F
DF
Kossoy
Nadja
N
Zusman
Itshak
I
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000276:immunology*; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control; D051379:Mice; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology* / Q000517:prevention & control; D010051:Ovarian Neoplasms / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control; D013997:Time Factors; D014594:Uterine Neoplasms / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D015127:9,10-Dimethyl-1,2-benzanthracene