Mobilization of CD34(+), CD117(+), CXCR4(+), c-met(+) stem cells is correlated with left ventricular ejection fraction and plasma NT-proBNP levels in patients with acute myocardial infarction.
OBJECTIVE
The aim of the study was to assess the correlation between the number of CD34(+), CD117(+), c-met(+), CXCR4(+) stem cells mobilized into peripheral blood, left ventricular ejection fraction (LVEF), NT-proBNP levels, and myocardial necrosis markers in patients with acute myocardial infarction (AMI).


RESULTS
43 patients with STEMI were enrolled. Stem cells number was measured using flow-cytometer and concentrations of NT-proBNP, SDF-1, G-CSF, VEGF, IL-6, and HGF were measured using ELISA kits. The number of stem cells mobilized early (<12 h) in AMI was significantly, positively correlated with LVEF: r=0.49 (P=0.0012) for CD34(+) cells, r=0.48 (P=0.0018) for CXCR4(+) cells, r=0.45 (P=0.0043) for CD117(+) cells, and r=0.41 (P=0.01) for c-met(+) cells and negatively correlated with NT-proBNP levels on admission r=-0.35 (P=0.024) for CD34(+) cells, r=-0.42 (P=0.007) for CXCR4(+) cells, r=-0.33 (P=0.04). In patients with LVEF </=40%, the peak number of CD34(+), CXCR4(+), CD117(+), and c-met(+) stem cells was significantly lower when compared patients with LVEF >40%. The number of CXCR4(+) cells on admission and after 24 h was negatively correlated with respective cardiac Troponin I levels (r=-0.37; P=0.029 and r=-0.45, P=0.02) and maximum activity of CK-MB (r=-0.37; P=0.021). No significant correlations between levels of haematopoietic cytokines and LVEF were found.


CONCLUSIONS
The mobilization of CD34(+), CD117(+), CXCR4(+), c-met(+) stem cells into peripheral blood early in STEMI is positively correlated with LVEF and negatively correlated with NT-proBNP levels and myocardial necrosis markers.
Wojakowski
Wojciech
W
3rd Division of Cardiology, Silesian School of Medicine, 45-47 Ziołowa Street, 40-635 Katowice, Poland. wojwoj@mp.pl
Tendera
Michal
M
Zebzda
Anna
A
Michalowska
Anna
A
Majka
Marcin
M
Kucia
Magdalena
M
Maslankiewicz
Katarzyna
K
Wyderka
Rafal
R
Król
Marek
M
Ochala
Andrzej
A
Kozakiewicz
Krystyna
K
Ratajczak
Mariusz Z
MZ
D018952:Antigens, CD34 / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000378:metabolism* / Q000503:physiopathology; D020097:Natriuretic Peptide, Brain / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013318:Stroke Volume
D018952:Antigens, CD34; D015415:Biomarkers; D010446:Peptide Fragments; D019718:Receptors, CXCR4; D011956:Receptors, Cell Surface; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain; D019009:Proto-Oncogene Proteins c-kit; D019859:Proto-Oncogene Proteins c-met