Design for DNA separation medium using bacterial cellulose fibrils.
In this paper, we present a novel DNA separation medium using bacterial cellulose fibrils. Bacterial cellulose has an intrinsic three-dimensional micrometer- to nanometer-scale network structure. Addition of this material to a low-concentration polymer solution (<5 cP) enables high-resolution electrophoretic separation of DNA, even for fragments of 10-100-bp or single-nucleotide polymorphism. The newly designed medium consists of a double mesh: a 10-nm flexible mesh derived from a conventional polymer medium containing 10-nm to 1-microm rigid pores made up of 10-microm bacterial cellulose fragments.
Tabuchi
Mari
M
Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Science, The University of Tokushima COE, 1-78 Shomachi, Tokushima 770-8505, Japan. tabuchi@ph.tokushima-u.ac.jp
Baba
Yoshinobu
Y
D000091:Acetobacter / Q000235:genetics*; D002482:Cellulose / Q000737:chemistry* / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification*; D049329:Nanostructures; D018807:Polymorphism, Single-Stranded Conformational
D002482:Cellulose; D004247:DNA