title: Human embryonic stem cells: towards therapies for cardiac disease. Derivation of a Dutch human embryonic stem cell line.
abstract: Cell transplantation is being discussed as a potential therapy for multiple disorders caused by loss or malfunction of single or at most a few cell types. These include diabetes, Parkinson's disease and myocardial infarction or cardiac failure. However, it is not yet clear whether cells from adult tissues ('adult stem cells') or embryos ('embryonic stem cells') will prove to be the most appropriate replacement cells; most likely, each disease will have its own preferred source. This study presents the background to this discussion and the current state of research in replacement of cardiac tissue, with focus on recent developments using human embryonic stem cells. It also describes a new human embryonic stem cell (HESC) line, NL-HESC1, the first to be derived in the Netherlands, and shows that it forms cardiac cells in a manner comparable with that of hES2 and hES3 cells grown in the same laboratory.
authors.affiliation: Hubrecht Laboratory, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Anja
authors.initials: A
authors.lastname: van de Stolpe
authors.forename: Stieneke
authors.initials: S
authors.lastname: van den Brink
authors.forename: Marga
authors.initials: M
authors.lastname: van Rooijen
authors.forename: Dorien
authors.initials: D
authors.lastname: Ward-van Oostwaard
authors.forename: Wouter
authors.initials: W
authors.lastname: van Inzen
authors.forename: Ineke
authors.initials: I
authors.lastname: Slaper-Cortenbach
authors.forename: Bart
authors.initials: B
authors.lastname: Fauser
authors.forename: Nijske
authors.initials: N
authors.lastname: van den Hout
authors.forename: Sjerp
authors.initials: S
authors.lastname: Weima
authors.forename: Robert
authors.initials: R
authors.lastname: Passier
authors.forename: Nigel
authors.initials: N
authors.lastname: Smith
authors.forename: Chris
authors.initials: C
authors.lastname: Denning
authors.forename: Christine
authors.initials: C
authors.lastname: Mummery
mesh_terms: D000818:Animals; D003585:Cell Biology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D017690:Cell Transplantation; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D006331:Heart Diseases / Q000628:therapy*; D006801:Humans; D051379:Mice; D009203:Myocardial Infarction / Q000628:therapy; D032383:Myocytes, Cardiac / Q000166:cytology*; D009426:Netherlands; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors