A polypyrrole protein microarray for antibody-antigen interaction studies using a label-free detection process.
Protein microarray is a promising technology that should combine rapidity and easy use with high throughput and versatility. This article describes a method in which an electrocopolymerization process is employed to graft biological molecules on to a chip so that surface plasmon resonance imaging may be used to detect molecular interactions. Copolymerization of pyrrole-modified protein and pyrrole is an efficient grafting process which immobilizes molecules at defined positions on a gold surface. Surface plasmon resonance imaging is an optical technique that allows real-time simultaneous detection of molecular interactions on a large number of spots without labeling. This method was successfully used to analyze antibody-antigen interactions. This illustrates its high specificity and good sensitivity and demonstrates its suitability for biological studies.
Grosjean
Ludivine
L
CREAB, UMR 5819 (CEA, CNRS, UJF), CEA-G/DRFMC, 17 rue des Martyrs, 38054 Grenoble, France.
Cherif
Boutheina
B
Mercey
Emilie
E
Roget
André
A
Levy
Yves
Y
Marche
Patrice Noel
PN
Villiers
Marie-Bernadette
MB
Livache
Thierry
T
D000818:Animals; D000937:Antigen-Antibody Reactions; D002623:Chemistry Techniques, Analytical; D006063:Chorionic Gonadotropin / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D009113:Muramidase / Q000276:immunology; D011108:Polymers; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011758:Pyrroles; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D006063:Chorionic Gonadotropin; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D009113:Muramidase