Quantification of tipranavir in human plasma by high-performance liquid chromatography with UV detection.
A simple method for the quantification of tipranavir, a new non-peptidic protease-inhibitor, was developed. An internal standard, prazepam, was added to 100 microl of plasma before a liquid-liquid extraction by 3 ml of tert-butyl methyl ether. The extracts were evaporated to dryness and reconstituted with 100 microl of mobile phase before being injected in the chromatographic system. The separation was made on a C8 column using sodium acetate buffer (pH 5):methanol:acetonitrile (35:30:35, v/v/v) as mobile phase. The detection was performed at a wavelength of 260 nm. The method was linear and has been validated over a concentration range of 2-80 mg/l. The mean precision and accuracy of the method were respectively, 10.5 and -9.1%. The mean recovery was 70.8%.
Giraud
Emmanuelle
E
Université Paris-Descartes, Faculté de Médecine, Assistance Publique-Hôpitaux de Paris, Groupe Hospitalier Cochin-Saint-Vincent de Paul, Pharmacologie Clinique, France.
Rey
Elisabeth
E
Tréluyer
Jean-Marc
JM
Pons
Gérard
G
Jullien
Vincent
V
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017320:HIV Protease Inhibitors / Q000097:blood*; D006801:Humans; D011725:Pyridines / Q000097:blood*; D011753:Pyrones / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013449:Sulfonamides
D017320:HIV Protease Inhibitors; D011725:Pyridines; D011753:Pyrones; D013449:Sulfonamides; C107201:tipranavir