A protease stable in organic solvents from solvent tolerant strain of Pseudomonas aeruginosa.
A solvent tolerant strain of Pseudomonas aeruginosa (PseA) was isolated from soil samples by cyclohexane enrichment in medium. The strain was able to sustain and grow in a wide range of organic solvents. The adaptation of P. aeruginosa cell towards solvents was seen at membrane level in transmission electron micrographs. It also secreted a novel protease, which exhibited remarkable solvent stability and retained most of the activity at least up to 10 days in the presence of hydrophobic organic solvents (log P > or = 2.0) at 25% (v/v) concentrations. The protease was able to withstand as high as 75% concentration of solvents at least up to 48 h. P. aeruginosa strain and its protease, both seem promising for solvent bioremediation, wastewater treatment and carrying out biotransformation in non-aqueous medium.
Gupta
Anshu
A
Department of Chemistry, Indian Institute of Technology, Delhi, Hauz-Khas, New Delhi.
Khare
S K
SK
D049109:Cell Proliferation; D003510:Cyclohexanes / Q000737:chemistry; D004795:Enzyme Stability; D046529:Microscopy, Electron, Transmission; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D012988:Soil Microbiology; D012997:Solvents / Q000494:pharmacology*
D003510:Cyclohexanes; D012997:Solvents; D010447:Peptide Hydrolases