title: A reference map of the Arabidopsis thaliana mature pollen proteome.
abstract: The male gametophyte (or pollen) plays an obligatory role during sexual reproduction of higher plants. The extremely reduced complexity of this organ renders pollen a valuable experimental system for studying fundamental aspects of plant biology such as cell fate determination, cell-cell interactions, cell polarity, and tip-growth. Here, we present the first reference map of the mature pollen proteome of the dicotyledonous model plant species, Arabidopsis thaliana. Based on two-dimensional gel electrophoresis, matrix-assisted laser desorption/ionization time-of-flight, and electrospray quadrupole time-of-flight mass spectrometry, we reproducibly identified 121 different proteins in 145 individual spots. The presence, subcellular localization, and functional classification of the identified proteins are discussed in relation to the pollen transcriptome and the full protein complement encoded by the nuclear Arabidopsis genome.
authors.affiliation: Max-Planck-Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829 Köln, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Noir
authors.forename: Anne
authors.initials: A
authors.lastname: Bräutigam
authors.forename: Thomas
authors.initials: T
authors.lastname: Colby
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schmidt
authors.forename: Ralph
authors.initials: R
authors.lastname: Panstruga
mesh_terms: D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D010455:Peptides / Q000378:metabolism; D011058:Pollen / Q000254:growth & development* / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D029681:Arabidopsis Proteins; D010455:Peptides; D020033:Protein Isoforms; D020543:Proteome