[Effects of plant growth substances on induction and culture of callus from Rhodiola quadrifida].
OBJECTIVE
To investigate the effect of plant growth substances on induction and culture of callus from Rhodiola quadrifida and also to analyze salidroside contents in the callus.


METHODS
The optimum combination of plant growth substances in MS solid medium for induction and culture of callus was established using orthogonal design. The contents of salidroside was analyzed by HPLC.


RESULTS
MS medium containing 2,4-D 1 mg x L(-1), NAA 2 mg x L(-1), 6-BA 0.5 mg x L(-1) and KT 0.1 mg x L(-1) could induce the callus from R. quadrifida most effectively;the induction rate was 83.3%. The optimized combination of plant growth substances for callus subculture was 2,4-D 1 mg x L(-1), 6-BA 0.1 mg x L(-1) and KT 0.5 mg x L(-1). The dry weight could reach 11.77 g x L(-1) when the callus was cultured in the optimum medium for 30 d and salidroside content was 0.28%.


CONCLUSIONS
The quantities of plant growth substances required for induction and culture of callus are different in R. quadrifida. The callus could produce salidroside.
Sheng
Chang-zhong
CZ
Tianjin Tasly Group Co. Ltd., Tianrin 300402, China.
Hu
Tie-qiang
TQ
Bi
Hao
H
Yuan
Ying-jin
YJ
Jiang
Yan
Y
D003470:Culture Media; D005960:Glucosides / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018547:Plant Stems / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D029771:Rhodiola / Q000254:growth & development* / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods*
D003470:Culture Media; D005960:Glucosides; D010636:Phenols; D010937:Plant Growth Regulators; C009172:rhodioloside