[Determination of oxymatrine content and its related substances in oxymatrine capsule by HPLC].
OBJECTIVE
To determine oxymatrine content and its related substances in oxymatrine capsule.


METHODS
A HPLC method was adopted. C18 column was used. The mobile phase consisted of phosphate buffer solution-methanol (85:15). The UV detective wavelength was 210 nm.


RESULTS
The calibration curve of oxymatrine was linear in the range of 6.952 - 20.856 microg/ml (r = 0.9999). The calibration curve of N-oxysophocarpine was linear in the range of 0.1837 - 0.5510 microg/ml (r = 0.9997).


CONCLUSIONS
The method is simple, rapid and accurate.
Qiu
Wenwei
W
The Institute for Drug Control of Huizhou City, Guangdong Huizhou 516008.
Jian
Shujuan
S
Gong
Huikang
H
D000470:Alkaloids / Q000032:analysis*; D000998:Antiviral Agents / Q000737:chemistry; D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D011807:Quinolizines; D000093842:Matrines
D000470:Alkaloids; D000998:Antiviral Agents; D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; C046246:oxysophocarpine; D000093842:Matrines; C037573:oxymatrine