title: Blood volume measurement: The comparison of pulse dye densitometry and Dill and Costill's methods.
abstract: In many clinical situations, it is crucial to determine circulating blood volume (BV) easily and to repeat this measurement. The Dye DensitoGram Analyzer (DDG, Nihon Kohden Corp) measures semi-automatically BV, using an injection of IndoCyanine Green (ICG, 10 mg), and avoiding intermittent blood samples. The DDG was used during a 90-day microgravity simulation by Head-Down-Tilt bed rest (HDT) to measure BV and compared with the calculation of the plasma volume (PV) variations according to Dill and Costill's formula (DC). Seventeen healthy volunteers were included: 8 control subjects (Co) and 9 subjects submitted to a resistive exercise counter-measure (CM). Measurements were performed, one day before HDT, on days 3 and 90 of HDT and on day 9 after HDT. A double measurement of the BV was performed to assess the repeatability of this method. On the last day of HDT a significant decrease (p < 0.05) in the PV was noted with the DDG (Co: -12.3+/-5.7%, CM: -9.0+/-5.3%) and DC; (Co: -4.7+/-1.8%, CM: -6.8+/-2.5%). A good repeatability of the technique was shown with a low intrasubjects coefficient of variation (4.95+/-0.95%) and an acceptable intersubjects coefficient of variation (15.30+/-1.13%). No correlation was noted between DDG and DC (r2 = 0.27). The DDG gives a good repeatability, not affected by the microgravity exposure. Thanks to its capacity to measure accurately the BV within 7-10 min, this device presents major advantages for clinical use and research purpose.
authors.affiliation: Laboratoire de Physiologie, UMR-CNRS 6188, Faculté de Médecine d'Angers, Rue Haute de Reculée, 49045 Angers Cedex, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Belin de Chantemèle
authors.forename: Guillemette
authors.initials: G
authors.lastname: Gauquelin-Koch
authors.forename: Monique
authors.initials: M
authors.lastname: Duvareille
authors.forename: Natacha
authors.initials: N
authors.lastname: Pellet
authors.forename: Claude
authors.initials: C
authors.lastname: Gharib
authors.forename: Marc-Antoine
authors.initials: MA
authors.lastname: Custaud
mesh_terms: D000328:Adult; D001810:Blood Volume; D001811:Blood Volume Determination / Q000295:instrumentation* / Q000379:methods*; D004396:Coloring Agents; D003720:Densitometry; D006801:Humans; D007208:Indocyanine Green; D008297:Male; D018474:Weightlessness Simulation
chemical_list: D004396:Coloring Agents; D007208:Indocyanine Green