title: TATA-binding protein and TBP-associated factors during herpes simplex virus type 1 infection: localization at viral DNA replication sites.
abstract: Host RNA polymerase II (RNAP II) is responsible for viral transcription of the herpes simplex virus type 1 (HSV-1) genome and is relocalized to viral DNA replication compartments. Thus, we investigated whether TATA-binding protein (TBP) and TBP-associated factors (TAFs) are recruited to sites of viral transcription and replication and whether TBP/TAF expressions are influenced upon infection. The protein levels of TBP, hsTAF1/TAF(II)250, hsTAF4/TAF(II)135, and hsTAF5/TAF(II)100 were constant during the early phase of infection and started to decrease late during infection. Only for hsTAF7/TAF(II)55 we sometimes observed a decrease already at 4-8h postinfection (p.i.). Concomitantly with the relocalization of RNAP II, TBP and hsTAFs were redistributed to sites of viral DNA replication and transcription. In the absence of viral DNA replication TBP/hsTAFs were present in distinct nuclear dots, however, enlargement of the nuclear structures did not take place. Our results show that HSV-1 infection has no influence on the protein levels of TFIID components and leads to a redistribution of TBP and hsTAFs to prereplicative sites that enlarge to viral DNA replication compartments.
authors.affiliation: Max-Planck-Institute for Neurological Research, D-50931 Köln, Germany.
authors.forename: Ilja
authors.initials: I
authors.lastname: Quadt
authors.forename: Andrea Katharina
authors.initials: AK
authors.lastname: Günther
authors.forename: Daniel
authors.initials: D
authors.lastname: Voss
authors.forename: Mario
authors.initials: M
authors.lastname: Schelhaas
authors.forename: Dagmar
authors.initials: D
authors.lastname: Knebel-Mörsdorf
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry*; D002478:Cells, Cultured; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism; D005347:Fibroblasts; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D012319:RNA Polymerase II / Q000032:analysis; D035381:TATA-Binding Protein Associated Factors / Q000032:analysis*; D035181:TATA-Box Binding Protein / Q000032:analysis*; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D035381:TATA-Binding Protein Associated Factors; D035181:TATA-Box Binding Protein; D012319:RNA Polymerase II