Studies in oligonucleotide-based artificial nuclease systems. intramolecular copper (II) complex formation in an oligonucleotide bis-phenanthroline conjugate.
We have recently developed oligonucleotide based artificial nuclease (OBAN) systems based on 2'-O-methyloligoribonucleotides carrying a 2,9-dimethylphenanthroline x Zn(II) complex. These hybridize to an RNA molecule with bulge formation in the central region of the target and cleave the RNA target in a catalytic manner. When studying an 11-mer 2'-O-methyloligoribonucleotide carrying two 2,9-dimethylphenanthroline moieties, located 5 base pairs apart from each other, we found that this forms a cyclic structure in the presence of Cu2+ ions. This is due to intramolecular Cu(2,9-dimethylphenanthroline)2 complex formation, i.e., with the two ligands conjugated to the same oligonucleotide.
Ossipov
Dmitri A
DA
Division of Organic and Bioorganic Chemistry, MBB, Scheele Laboratory, Karolinska Intitutet, S-17177, Sweden.
Strömberg
Roger
R
D003300:Copper / Q000737:chemistry* / Q000494:pharmacology; D004583:Electrons; D007477:Ions; D008024:Ligands; D008670:Metals / Q000737:chemistry; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods; D008968:Molecular Conformation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D013053:Spectrophotometry; D013696:Temperature; D015032:Zinc / Q000737:chemistry
D007477:Ions; D008024:Ligands; D008670:Metals; D009841:Oligonucleotides; D010618:Phenanthrolines; D010710:Phosphates; D012313:RNA; D003300:Copper; D015032:Zinc; C029892:cupric chloride