[Experimental study on effect of baoganning on activity of nuclear transcription factor-kappaB in hepatic stellate cells].
OBJECTIVE
To investigate the effect of Baoganning (BGN) on activity of nuclear transcription factor-kappaB (NF-kappaB) in hepatic stellate cells (HSCs) and its relevant mechanisms.


METHODS
Normal Wistar rats were medicated with BGN decoction by gavage for 7 days to prepare BGN drug-serum. The effect of BGN drug-serum on HSC-T6 growth was measured by MTT assay; phosphorylation level of NF-kappaB inhibiting factor IkappaB at different time after BGN stimulation was detected by Western blotting analysis; and the binding level of NF-kappaB with DNA was measured 30 min after drug-serum stimulation with gel shift assay.


RESULTS
BGN could significantly inhibit the HSC-T6 growth and quickly supress the phosphorylation of IkappaB, with the effect reached its peak at 30 min and restored to baseline level 6 h after stimulation, and reduce the binding capacity of NF-kappaB with DNA.


CONCLUSIONS
BGN can inhibit phosphorylation of IkappaB, restrain the activity of NF-kappaB and change the binding level of NF-kappaB with DNA.
Tan
Yan
Y
Department of Traditional Chinese Medicine, Southern Medical University, Guangzhou.
Lu
Zhi-ping
ZP
Ou
Su-neng
SN
Zhang
Xufu
X
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D004247:DNA