Cocaine induction of ERK proteins in dorsal striatum of Fischer rats.
Cocaine is an addictive psychostimulant that induces fos and opioid gene expression by activating the dopamine receptors and the PKA pathways in dopamine D1 and a glutamate NMDA-dependent mechanisms in the striatum. In this study, we show that a single cocaine administration induces ERK phosphorylation in the caudate/putamen of Fischer rats. This increase in Phospho-ERK is diminished by pre-administration of SCH23390, or MK801 but not with pre-administration of eticlopride. Furthermore, this single cocaine administration does not alter the levels of phospho-CREB protein or CREB-DNA bindings in the caudate/putamen protein extracts but does increase phospho-Elk-1 protein levels in the same extracts.
Jenab
Shirzad
S
Department of Psychology, Hunter College of The City University of New York, NY 10021, USA.
Festa
Eugene D
ED
Nazarian
Arbi
A
Wu
Hui Bing K
HB
Sun
Wei Lun
WL
Hazim
Ruhal
R
Russo
Scott J
SJ
Quinones-Jenab
Vanya
V
D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D015153:Blotting, Western / Q000379:methods; D003042:Cocaine / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods; D004790:Enzyme Induction / Q000187:drug effects; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D012457:Salicylamides / Q000494:pharmacology
D001552:Benzazepines; D017362:Cyclic AMP Response Element-Binding Protein; D018492:Dopamine Antagonists; D018765:Dopamine Uptake Inhibitors; D018691:Excitatory Amino Acid Antagonists; D012457:Salicylamides; D016291:Dizocilpine Maleate; D020928:Mitogen-Activated Protein Kinases; D003042:Cocaine; C045989:eticlopride