title: Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions.
abstract: The molecular, cellular or tissue environment can influence the expression of genes and thereby regulate processes of tissue formation. Here we determined the tissue and serum dependence of the expression of all photopigments in the chick by a series of distinct retinal cell cultures, analyzed by RT-PCR using specific primers for all four opsins and rhodopsin followed by quantitative scanning of the respective gel bands. For comparison, we first determined expression of all opsins during normal chick retinogenesis, which began with red and violet opsins at E12, shortly followed by blue and green opsins and finally rhodopsin at E14. This period corresponds to the time of synaptogenesis in the inner retina. All cultures were started with 6-day-old dissociated retinal cells. Cells were kept at low or high cell density (called LoDens or HiDens), or they were reaggregated as retinal spheres, whereby all of them were raised at low (2%) or high serum (12%) levels (called LoSer or HiSer). In LoDens/HiSer cultures, expression of all opsins was weak. At HiDens/LoSer red and green opsin expression was strong, while rhodopsin and violet/blue remained low. In HiDens/HiSer cultures the expression of red and green was strong; rhodopsin was almost normal, while violet and green were low. In reaggregates at high serum the expression came closest to a normal retina, but violet and blue opsins were still below normal. At low serum, however, violet and blue were negligible and rhodopsin was low. This in vitro study shows that rhodopsin, followed by violet and blue opsin expressions is highly dependent on serum, cell density and tissue conditions, while red and green opsins are more autonomous.
authors.affiliation: Darmstadt University of Technology, Developmental Biology and Neurogenetics, Darmstadt, Germany.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Jacob
authors.forename: Andrée
authors.initials: A
authors.lastname: Rothermel
authors.forename: Peter
authors.initials: P
authors.lastname: Wolf
authors.forename: Paul G
authors.initials: PG
authors.lastname: Layer
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D012160:Retina / Q000737:chemistry / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D012243:Rhodopsin / Q000235:genetics / Q000378:metabolism*; D017299:Rod Opsins / Q000235:genetics / Q000378:metabolism*; D044967:Serum / Q000378:metabolism
chemical_list: D003470:Culture Media; D017299:Rod Opsins; C095853:short-wavelength opsin; D012243:Rhodopsin