Biologically active lipid A antagonist embedded in a multilayered polyelectrolyte architecture.
Recently [Jessel N, Schwinte P, Donohue R, Lavalle P, Boulmedais F, Darcy R, et al. Pyridylamino-beta-cyclodextrin as a molecular chaperone for lipopolysaccharide embedded in a multilayered polyelectrolyte architecture. Adv Funct Mater 2004;14:963-9], we demonstrated the biological activity of a lipopolysaccharide from Escherichia coli incorporated into layer-by-layer films made of poly (l-lysine) and poly (l-glutamic acid) and containing a polycationic beta-cyclodextrin (CD) with chaperone properties. Here we develop innovative architectures containing a complex made of a charged beta-cyclodextrin and a lipid A antagonist (LAA) as potential systems for local endotoxin antagonistic activity. We examine the biological activity of these architectures. The CD-LAA complex adsorbed on top, or embedded into the polyelectrolyte films keeps its LPS antagonistic activity on both murine and human macrophages for at least 24h.
Gangloff
Sophie C
SC
Immuno-Pharmacologique Cellulaire et Moléculaire, EA3796-IFR53, UFR Pharmacie, 51 rue Cognacq Jay 51100 Reims, France.
Ladam
Guy
G
Dupray
Valérie
V
Fukase
Kochi
K
Brandenburg
Klaus
K
Guenounou
Moncef
M
Schaaf
Pierre
P
Voegel
Jean-Claude
JC
Jessel
Nadia
N
D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D008050:Lipid A / Q000037:antagonists & inhibitors* / Q000737:chemistry; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D008809:Mice, Inbred C3H; D010455:Peptides / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry / Q000494:pharmacology*; D000071228:Polyelectrolytes; D013059:Spectrum Analysis, Raman; D047392:beta-Cyclodextrins / Q000737:chemistry
D008050:Lipid A; D010455:Peptides; D011073:Polyamines; D000071228:Polyelectrolytes; D047392:beta-Cyclodextrins; C031215:betadex