title: Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring.
abstract: Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H(2)O(2)-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as pH dependency, I(max), K(M)(app), detection limit, linear range, operational and storage stability. The obtained results showed a distinctly different behavior for the plant peroxidase electrodes, demonstrating uniquely superior characteristics of the RPTP-based sensors. The broader linear range observed for the RPTP-based biosensor is explained by a high stability of this enzyme in presence of H(2)O(2). The higher storage and operational stability of RPTP-based biosensor as well as its capability to measure hydrogen peroxide under acidic conditions connect with an extremely high thermal and pH-stability of RPTP.
authors.affiliation: Department of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow 119992, Russia.
authors.forename: Inna S
authors.initials: IS
authors.lastname: Alpeeva
authors.forename: Mihaela
authors.initials: M
authors.lastname: Niculescu-Nistor
authors.forename: Jaime Castillo
authors.initials: JC
authors.lastname: Leon
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Csöregi
authors.forename: Ivan Yu
authors.initials: IY
authors.lastname: Sakharov
mesh_terms: D028023:Arecaceae / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010544:Peroxidases / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004800:Enzymes, Immobilized; D006861:Hydrogen Peroxide; D010544:Peroxidases