title: NestinnegCD24low/- population from fetal Nestin-EGFP transgenic mice enriches the pancreatic endocrine progenitor cells.
abstract: OBJECTIVE
  To identify whether Nestin-positve cells or Nestin-negative cells in pancreas enrich potential pancreatic stem/progenitor cells.
  
  
  METHODS
  We generated transgenic mice carrying enhanced green fluorescent protein (EGFP) under the control of the nestin second-intronic enhancer and subsequently divided their embryonic pancreatic cells into different subpopulations according to the expression of EGFP and CD24 and characterized these subpopulations by in vitro culture.
  
  
  RESULTS
  The EGFP expression correlated well with that of endogenous Nestin. Only the NestinCD24 subpopulation was able to proliferate and generate immature islet-like cell clusters in long-term culture. Immature islet-like cell clusters could be induced to differentiate into insulin-, glucagon-, and somatostatin-positive cells.
  
  
  CONCLUSIONS
  Pancreatic endocrine stem/progenitor cells are enriched in the NestinCD24 population of embryonic pancreas.
authors.affiliation: PKU-BLARC Mouse Genomics Center, Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Wang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Hu
authors.forename: Dongxin
authors.initials: D
authors.lastname: Zhao
authors.forename: Guangwen
authors.initials: G
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Tan
authors.forename: Liying
authors.initials: L
authors.lastname: Du
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Lingling
authors.initials: L
authors.lastname: Hou
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Yuansong
authors.initials: Y
authors.lastname: Yu
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Hongkui
authors.initials: H
authors.lastname: Deng
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Ding
mesh_terms: D000818:Animals; D015415:Biomarkers; D051927:CD24 Antigen / Q000032:analysis*; D002454:Cell Differentiation; D049109:Cell Proliferation; D049452:Green Fluorescent Proteins / Q000032:analysis; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis*; D007515:Islets of Langerhans / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000032:analysis*; D064231:Nestin; D010179:Pancreas / Q000196:embryology; D013234:Stem Cells / Q000166:cytology*
chemical_list: D015415:Biomarkers; D051927:CD24 Antigen; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D049452:Green Fluorescent Proteins