title: Ectomycorrhizal symbiosis in vitro between Tricholoma matsutake and Pinus densiflora seedlings that resembles naturally occurring 'shiro'.
abstract: We established an in vitro ectomycorrhizal symbiosis between Tricholoma matsutake and Pinus densiflora. Mycorrhiza formed in a substrate of Modified Norkrans' C medium and granite-based soil had features similar to those observed previously only in naturally occurring mycorrhizal system called 'shiro,' and promoted the growth of plants with smaller root/shoot ratios. The in vitro formation of 'shiro' is essential for the development of T. matsutake system to produce mushrooms and is useful for the propagation and plantation of the mycorrhizal seedlings.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Minami-minowa, Nagano, 399-4598, Japan. akiyosh@gipmc.shinshu-u.ac.jp.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Yamada
authors.affiliation: Ibaraki Prefectural Forestry Institute, Naka, Ibaraki, 311-0122, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
authors.affiliation: Ibaraki Prefectural Forestry Institute, Naka, Ibaraki, 311-0122, Japan.
authors.forename: Hisayasu
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, 305-8687, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Murata
mesh_terms: D003470:Culture Media; D038821:Mycorrhizae / Q000502:physiology*; D028223:Pinus / Q000254:growth & development / Q000382:microbiology*; D012822:Silicon Dioxide; D013559:Symbiosis
chemical_list: D003470:Culture Media; C007886:granite; D012822:Silicon Dioxide