title: Oxidative stress in the erythrocytes of cattle intoxicated with Senecio sp.
abstract: BACKGROUND
  Intoxication caused by Senecio sp is characterized by irreversible damage to liver cells and may be associated with oxidative stress.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the effects of intoxication by Senecio sp on lipoperoxidation, antioxidant defenses, and the osmotic resistance of erythrocytes in cattle.
  
  
  METHODS
  Blood samples from 30 intoxicated animals (group 1) and 30 healthy animals (group 2) were analyzed. The diagnosis of poisoning by Senecio sp was based on histopathologic lesions verified through hepatic biopsy. The following biochemical parameters of oxidative stress in the erythrocytes were determined: thiobarbituric acid-reactive substances (TBARS), copper-zinc superoxide dismutase (CuZnSOD) activity, and nonprotein sulfhydryl (NPSH) groups. Erythrocyte osmotic fragility also was evaluated.
  
  
  RESULTS
  TBARS concentration and CuZnSOD activity were significantly (P <.001) higher in group 1 when compared with group 2. The concentration of erythrocyte NPSH groups was significantly (P <.03) lower in group 1 when compared with group 2. Osmotic fragility was more pronounced in the erythrocytes of group 1 when compared with group 2 (P < .001).
  
  
  CONCLUSIONS
  The results of this study indicate that poisoning by Senecio sp causes an increase in lipoperoxidation, oxidation of NPSH groups, and consequently, oxidative stress in bovine erythrocytes that may contribute to hemolysis. These findings may contribute to a better understanding of the mechanisms involved in cell damage in animals intoxicated by Senecio sp.
authors.affiliation: Departamento de Clínica Médica, Universidade Federal de Santa Maria, Santa Maria RS, Brasil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Bondan
authors.forename: Julio Cesar Mendes
authors.initials: JC
authors.lastname: Soares
authors.forename: Marcelo
authors.initials: M
authors.lastname: Cecim
authors.forename: Sonia Terezinha dos Anjos
authors.initials: ST
authors.lastname: Lopes
authors.forename: Dominguita Lühers
authors.initials: DL
authors.lastname: Graça
authors.forename: Ricardo Xavier
authors.initials: RX
authors.lastname: da Rocha
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000503:physiopathology; D003300:Copper / Q000097:blood; D004912:Erythrocytes / Q000737:chemistry / Q000502:physiology*; D006461:Hemolysis; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008107:Liver Diseases / Q000097:blood / Q000209:etiology / Q000662:veterinary*; D009996:Osmotic Fragility; D018384:Oxidative Stress; D012674:Senecio / Q000506:poisoning*; D013438:Sulfhydryl Compounds / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D015032:Zinc / Q000097:blood
chemical_list: D000975:Antioxidants; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc