Regional difference in corticotropin-releasing factor immunoreactivity in mossy fiber terminals innervating calretinin-immunoreactive unipolar brush cells in vestibulocerebellum of rolling mouse Nagoya.
Unipolar brush cells (UBCs), a class of interneurons in the vestibulocerebellum, play roles in amplifying excitatory inputs from vestibulocerebellar mossy fibers. This study aimed to clarify whether corticotropin-releasing factor (CRF)-positive mossy fiber innervation of calretinin (CR)-positive UBCs was altered in rolling mouse Nagoya (RMN). The distribution and the number of CR-positive UBCs in the vestibulocerebellum were not different between RMN and control mice. Double immunofluorescence revealed that some CRF-positive mossy fiber terminals were in close apposition to CR-positive UBCs. In the lobule X of vermis, such mossy fiber terminals were about 5-fold greater in number in RMN than in controls. In contrast, the number of CRF-positive mossy fiber terminals adjoining CR-positive UBCs in the flocculus was not significantly different between RMN and controls. The results suggest increased number of CRF-positive mossy fiber terminals innervating CR-positive UBCs in the lobule X but not in the flocculus of RMN. CRF may alter CR-positive UBC-mediated excitatory pathways in the lobule X of RMN and may disturb functions of the lobule X such as cerebellar adaptation for linear motion of the head.
Ando
Masahiro
M
Department of Otolaryngology, University of Tokushima Graduate School Institute of Health Biosciences, Tokushima 770-8503, Japan.
Sawada
Kazuhiko
K
Sakata-Haga
Hiromi
H
Jeong
Young-Gil
YG
Takeda
Noriaki
N
Fukui
Yoshihiro
Y
D000818:Animals; D064032:Calbindin 2; D002524:Cerebellar Ataxia / Q000235:genetics / Q000378:metabolism*; D002525:Cerebellar Cortex / Q000166:cytology / Q000378:metabolism*; D002529:Cerebellar Nuclei / Q000166:cytology / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007395:Interneurons / Q000378:metabolism*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008818:Mice, Neurologic Mutants; D009412:Nerve Fibers / Q000378:metabolism*; D009434:Neural Pathways / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*
C576511:Calb2 protein, mouse; D064032:Calbindin 2; D064030:S100 Calcium Binding Protein G; D003346:Corticotropin-Releasing Hormone