title: Identification of genes preferentially expressed in periodontal ligament: specific expression of a novel secreted protein, FDC-SP.
abstract: Gene expression in human periodontal ligament (PDL) was examined by suppression subtractive hybridization to identify genes that are preferentially expressed in tissue compared to cultured PDL fibroblasts. The most enriched genes in a subtracted cDNA library are primarily genes for extracellular matrix components, types I and III collagen, lumican, periostin, and asporin, among others, whose expression conveys unique mechanical properties to the PDL. Also within this group is the gene for follicular dendritic cell secreted protein (FDC-SP), a small protein like statherin in saliva, not previously found in PDL. FDC-SP's presence in PDL was confirmed by in situ hybridization in mouse which also showed that it was definitely present in the parotid gland, but, surprisingly, not in the other salivary glands: submandibular and sublingual. Since only normal tissue was examined, these findings suggest that FDC-SP plays an important but previously unsuspected role within oral connective tissue.
authors.affiliation: Tissue Regeneration, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Nakamura
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Terashima
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yoshida
authors.forename: Sachiko
authors.initials: S
authors.lastname: Iseki
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Takano
authors.forename: Isao
authors.initials: I
authors.lastname: Ishikawa
authors.forename: Tamayuki
authors.initials: T
authors.lastname: Shinomura
mesh_terms: D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D010513:Periodontal Ligament / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D016326:Extracellular Matrix Proteins; C465580:FDCSP protein, human; D011506:Proteins; D020543:Proteome