title: Oxidation of biotin during oligonucleotide synthesis.
abstract: A new "polystyrene biotin support" has been synthesized for the solid support synthesis of the 3'-biotinylated oligonucleotides. Several oligos were synthesized and were analyzed by the HPLC and Mass Spec. Oligo analysis revealed that the biotin gets oxidized to "biotin sulfoxide" during the synthesis.
authors.affiliation: Applied Biosystems, 8a50 Lincoln Centre Drive, Foster City, CA 94404, USA. upadhykg@appliedbiosystems.com
authors.forename: Krishna
authors.initials: K
authors.lastname: Upadhya
authors.forename: Iqbal K
authors.initials: IK
authors.lastname: Khattak
authors.forename: Bashar
authors.initials: B
authors.lastname: Mullah
mesh_terms: D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry*; D019921:Biotinylation; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*
chemical_list: D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D001710:Biotin; C017072:D-biotin-d-sulfoxide; D010100:Oxygen