title: A tRNA(Glu) that uncouples protein and tetrapyrrole biosynthesis.
abstract: Glu-tRNA is either bound to elongation factor Tu to enter protein synthesis or is reduced by glutamyl-tRNA reductase (GluTR) in the first step of tetrapyrrole biosynthesis in most bacteria, archaea and in chloroplasts. Acidithiobacillus ferrooxidans, a bacterium that synthesizes a vast amount of heme, contains three genes encoding tRNA(Glu). All tRNA(Glu) species are substrates in vitro of GluRS1 from A. ferrooxidans.Glu-tRNA(3)(Glu), that fulfills the requirements for protein synthesis, is not substrate of GluTR. Therefore, aminoacylation of tRNA(3)(Glu) might contribute to ensure protein synthesis upon high heme demand by an uncoupling of protein and heme biosynthesis.
authors.affiliation: Programa de Biologia Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 838-0453, Chile.
authors.forename: Gloria
authors.initials: G
authors.lastname: Levicán
authors.forename: Assaf
authors.initials: A
authors.lastname: Katz
authors.forename: Patricio
authors.initials: P
authors.lastname: Valenzuela
authors.forename: Dieter
authors.initials: D
authors.lastname: Söll
authors.forename: Omar
authors.initials: O
authors.lastname: Orellana
mesh_terms: D042763:Acidithiobacillus / Q000235:genetics / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D005798:Genes, Bacterial / Q000502:physiology; D005975:Glutamate-tRNA Ligase / Q000378:metabolism; D010444:Peptide Elongation Factor Tu / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics; D012352:RNA, Transfer, Glu / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D045725:Tetrapyrroles / Q000096:biosynthesis*; D046249:Transfer RNA Aminoacylation
chemical_list: D012352:RNA, Transfer, Glu; D045725:Tetrapyrroles; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase; D010444:Peptide Elongation Factor Tu; D005975:Glutamate-tRNA Ligase