Accurate mass analysis of phosphoramidites by electrospray mass spectrometry.
A method of accurate mass determination of phosphoramidites is described. The commonly used methanol/water/acid system was replaced by LiCl-containing acetonitrile and the concentrations of LiCl, poly(ethylene glycol), and phosphoramidite samples were optimized.
Kupihár
Z
Z
Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado, USA. kupihar@colorado.edu
Timár
Z
Z
Dellinger
D J
DJ
Caruthers
M H
MH
D000097:Acetonitriles / Q000737:chemistry / Q000494:pharmacology; D018021:Lithium Chloride / Q000737:chemistry / Q000494:pharmacology; D046911:Macromolecular Substances; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000097:Acetonitriles; D046911:Macromolecular Substances; D009943:Organophosphorus Compounds; D011108:Polymers; C434331:phosphoramidite; D011092:Polyethylene Glycols; D018021:Lithium Chloride; C032159:acetonitrile