[Phosphatase activity of Bacillus subtilis IMV B-7023].
Phosphatase activity of two strains of bacteria - Bacillus subtilis IMV B-7023 and B. megaterium 12 is investigated. The phosphatase activity is found to reach 260 mkmol/g x hour for B. subtilis IMV B-7023 and 12-100 mkmol/g x hour for B. megaterium 12 at optimal temperature (55 degrees C) and pH (9.5-10.0). Synthesis of alkaline phosphatase is shown to reach its maximum values at the end of logarithmic phase of the culture growth. It is revealed that Mg2+, Ca2+ cations increase phosphotase activity of B. subtilis IMV B-7023, at the same time Cu2+, Mn2+, Zn2+ cations and inorganic phosphate decrease it. Dependence of the rate of phosphatase reaction of B. subtilis IMV B-7023 on substrate concentration is determined.
Bulavenko
L V
LV
Kurdysh
I K
IK
D000469:Alkaline Phosphatase / Q000378:metabolism*; D001410:Bacillus megaterium / Q000201:enzymology; D001412:Bacillus subtilis / Q000201:enzymology*; D002021:Buffers; D002413:Cations, Divalent / Q000494:pharmacology; D003470:Culture Media; D004789:Enzyme Activation; D007700:Kinetics; D013379:Substrate Specificity; D013696:Temperature
D002021:Buffers; D002413:Cations, Divalent; D003470:Culture Media; D000469:Alkaline Phosphatase