title: Effect of different phenolic compounds on alpha-amylase activity: screening by microplate-reader based kinetic assay.
abstract: The inhibitory effect of different polyphenolic plant compounds on alpha-amylase activity was investigated in vitro. A kinetic assay was performed using 96-well-plates. Acarbose was used as positive control (IC50: 23.2 microM). Some of the tested compounds, occurring in plants traditionally used in anti-diabetic tea species, showed an inhibition of the enzyme in physiological concentrations, e.g. luteolin, tannic acid, and isochlorogenic acid.
authors.affiliation: Institut für Pharmazie, Freie Universität, Berlin, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Funke
authors.forename: M F
authors.initials: MF
authors.lastname: Melzig
mesh_terms: D020909:Acarbose / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology*; D007202:Indicators and Reagents; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology; D007700:Kinetics; D008519:Medicine, Traditional; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D005419:Flavonoids; D007004:Hypoglycemic Agents; D007202:Indicators and Reagents; D010636:Phenols; D059808:Polyphenols; D000516:alpha-Amylases; D020909:Acarbose