A novel amino-on CPG-support for the synthesis of 3'-aminoalkylated oligonucleotides.
The synthesis of a novel amino-ON CPG support and its application in the synthesis of 3'-aminoalkylated oligonucleotides is reported. The release of oligonucleotides with free 3'-amino groups is accomplished by treatment with concentrated ammonia for 2 h at 55 degrees C.
Leuck
Michael
M
Proligo Biochemie GmbH, Georg-Heyken-Str. 14, Hamburg 21149, Germany.
Glare
Rubina
R
Zien
Nicole
N
Paul
Matthias
M
Wolter
Andreas
A
D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D018899:CpG Islands; D003432:Cross-Linking Reagents / Q000494:pharmacology; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
C408982:CPG-oligonucleotide; D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D009841:Oligonucleotides