Structure of lintnerized starch is related to X-ray diffraction pattern and susceptibility to acid and enzyme hydrolysis of starch granules.
Acid-resistant residues (lintnerized starches, Ls) were prepared from starches showing A-, B- and C- X-ray diffraction patterns. Ls retained the same X-ray crystalline type as their native counterparts with an improvement in diffraction intensity. Fluorophore-assisted capillary electrophoresis (FACE) study indicated that structural characteristics of Ls were associated with X-ray diffraction patterns. Double helices originated from linear chains with an approximate average degree of polymerisation (DP) 14, 16, and 15 would span the entire length of crystalline lamellae of A-, B-, and C-type starches, respectively. The proportion of singly branched materials (SB) with DP 25 protected in Ls was higher for A-type Ls (10-17%) than for B-type Ls (4-6%) and C-type Ls (8%). The structures of SB were similar in which branched chain (DP 13-15) was longer than main chain (DP 10-12). The structural characteristics of Ls are discussed in relation to acid and enzymatic degradations of starch granules.
Srichuwong
Sathaporn
S
Department of Sustainable Resource Sciences, Faculty of Bioresources, Mie University, Tsu 514-8507, Japan.
Isono
Naoto
N
Mishima
Takashi
T
Hisamatsu
Makoto
M
D000143:Acids / Q000494:pharmacology; D000818:Animals; D001688:Biological Products / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D004798:Enzymes / Q000737:chemistry; D006868:Hydrolysis; D046911:Macromolecular Substances / Q000737:chemistry*; D010179:Pancreas / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D013997:Time Factors; D014961:X-Ray Diffraction; D000516:alpha-Amylases / Q000737:chemistry
D000143:Acids; D001688:Biological Products; D004798:Enzymes; D046911:Macromolecular Substances; D011134:Polysaccharides; D013213:Starch; D000516:alpha-Amylases