[Immunogenicity of envelope glycoprotein gene of reticuloendotheliosis virus expressed in insect cell].
A recombinant baculovirus expressing reticuloendotheliosis virus env gene was constructed with Bac-to-Bac Baculovirus Expression Systems. After transfecting the recombinant virus into Sf9 cells for 3 days, REV env can be detected by indirect immunofluorescence antibody assay (IFA) and Western blot with specific monoclonal antibodies of REV. The oil-water emulsion vaccine was then produced using this infected Sf9 cells lycates and inoculated SPF chickens to validate the immunogenicity for REV. The results show that special anti-REV antibody can maintain more than 45 days and resist the infection of REV viruses. This is the first success to induce anti-REV antibody in chickens by none-live viruses.
Wang
Xi-Le
XL
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. wangxile80@163.com
Zhang
Zhi
Z
Jiang
Shi-Jin
SJ
Cui
Zhi-Zhong
ZZ
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015153:Blotting, Western; D002645:Chickens; D019084:Fluorescent Antibody Technique, Indirect; D015752:Genes, env; D011994:Recombinant Proteins / Q000276:immunology; D001356:Reticuloendotheliosis virus / Q000235:genetics / Q000276:immunology*; D018411:Spodoptera / Q000166:cytology / Q000235:genetics; D014614:Vaccines, Synthetic / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines