title: Characterization by Mass Spectroscopy of a 10 kDa c-554 Cytochrome from the Green Sulfur Bacterium Chlorobium tepidum.
abstract: Preparative isoelectric focusing was used to isolate a type c cytochrome from photosynthetic membranes of the green sulfur bacterium Chlorobium tepidum. The purified protein showed a molecular weight of 10 kDa according to SDS-PAGE and ESI mass spectrometry. The absorption spectrum in the visible range is typical of a cytochrome with peaks at 420, 525.2 and 554.4 nm. Cleavage by either trypsin or endoproteinase lys-C of the isolated cytochrome combined with tandem mass spectrometry and Edman sequencing yielded a sequence perfectly matching parts of the recently sequenced genome of C. tepidum.
authors.affiliation: Division of Biochemistry, Department of Chemistry, University of Crete, 71409, Heraklion, Greece.
authors.forename: Hervé-W
authors.initials: HW
authors.lastname: Rémigy
authors.forename: Michalis
authors.initials: M
authors.lastname: Aivaliotis
authors.forename: Nikos
authors.initials: N
authors.lastname: Ioannidis
authors.forename: Paul
authors.initials: P
authors.lastname: Jenö
authors.forename: Thierry
authors.initials: T
authors.lastname: Mini
authors.forename: Andreas
authors.initials: A
authors.lastname: Engel
authors.forename: Michel
authors.initials: M
authors.lastname: Jaquinod
authors.forename: Georgios
authors.initials: G
authors.lastname: Tsiotis