Polymorphisms of the BRCA2 and RAD51 genes in breast cancer.
We performed a case-control study (150 cases and 150 controls) to test the association between three polymorphisms in BRCA2 and RAD51 genes and breast cancer risk. Genotypes were determined in DNA from blood cells by PCR-RFLP. Cancer occurrence was strongly associated with the BRCA2 Met/1915Thr homozygous polymorphic variants, whereas heterozygous variant was associated with significant reduction in breast cancer risk. Gene-gene interaction between the BRCA2-Met1915Thr Thr/Thr and BRCA2-Met784Val Met/Met homozygous variants increased the risk. Therefore, the Met1915Thr polymorphism in the BRCA2 gene may be considered as an independent marker of breast cancer.
Sliwinski
Tomasz
T
Department of Molecular Genetics, University of Lodz, Lodz, Poland, januszb@biol.uni.lodz.pl.
Krupa
Renata
R
Majsterek
Ireneusz
I
Rykala
Jan
J
Kolacinska
Agnieszka
A
Morawiec
Zbigniew
Z
Drzewoski
Jozef
J
Zadrozny
Marek
M
Blasiak
Janusz
J
D024682:BRCA2 Protein / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D004247:DNA / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D011044:Poland; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D051135:Rad51 Recombinase / Q000235:genetics*; D044465:White People / Q000235:genetics
D024682:BRCA2 Protein; D004247:DNA; C495153:RAD51 protein, human; D051135:Rad51 Recombinase