title: Distribution of 10 periodontal bacteria in saliva samples from Japanese children and their mothers.
abstract: OBJECTIVE
  We analyzed the distribution of 10 periodontal bacteria species (Porphyromonas gingivalis, Tannerella forsythensis, Prevotella intermedia, Prevotella nigrescens, Campylobacter rectus, Eikenella corrodens, Actinobacillus actinomycetemcomitans, Capnocytophaga ochracea, Capnocytophaga sputigena, and Treponema denticola) in children, and then compared their distribution in those children and their mothers, with special attention given to three of the species known as the red complex (P. gingivalis, T. forsythensis, and T. denticola) whose presence has been shown to be associated with conditions related to periodontal diseases.
  
  
  METHODS
  One hundred thirteen pairs of children and their mothers were randomly selected from patients treated at the Pedodontic Clinic of Osaka University Dental Hospital. Saliva samples were taken at the second visit prior to receiving professional tooth brushing instruction. Genomic DNA was extracted from each saliva sample, followed by a polymerase chain reaction assay with species-specific sets of primers.
  
  
  RESULTS
  A. actinomycetemcomitans was the most frequently detected species in the mothers, followed by C. sputigena, P. gingivalis, and T. forsythensis, while C. sputigena had the highest detection rate, followed by A. actinomycetemcomitans and T. denticola in the children. The detection rate of the red complex species in children whose mothers possessed the same species was significantly higher than in those whose mothers did not possess them.
  
  
  CONCLUSIONS
  Our results indicate a correlation between the presence of periodontal bacteria in children and their mothers, while the presence of red complex bacteria in children was highly associated with that in their mothers.
authors.affiliation: Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1-8 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Tamura
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakano
authors.forename: Tetsuyuki
authors.initials: T
authors.lastname: Hayashibara
authors.forename: Ryota
authors.initials: R
authors.lastname: Nomura
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Fujita
authors.forename: Seikou
authors.initials: S
authors.lastname: Shintani
authors.forename: Takashi
authors.initials: T
authors.lastname: Ooshima
mesh_terms: D000293:Adolescent; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics; D001419:Bacteria / Q000235:genetics*; D041963:Bacteroidetes / Q000235:genetics; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D007564:Japan; D008297:Male; D008875:Middle Aged; D009035:Mothers; D010518:Periodontitis / Q000382:microbiology*; D010519:Periodontium / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D016966:Porphyromonas gingivalis / Q000235:genetics; D012044:Regression Analysis; D012463:Saliva / Q000382:microbiology*; D048373:Treponema denticola / Q000235:genetics
chemical_list: D004269:DNA, Bacterial