Deficiency of Bloom's syndrome protein causes hypersensitivity of C. elegans to ionizing radiation but not to UV radiation, and induces p53-dependent physiological apoptosis.
Caenorhabditis elegans him-6 mutants, which show a high incidence of males and partial embryonic lethality, are defective in the orthologue of human Bloom's syndrome protein (BLM). When strain him-6(e1104) containing a missense him-6 mutation was irradiated with gamma-rays during germ cell development or embryogenesis, embryonic lethality was higher than in the wild type, suggesting a critical function of the wild type gene in mitotic and pachytene stage germ cells as well as in early embryos. Even in the absence of gamma-irradiation, apoptosis was elevated in the germ cells of the him-6 strain and this increase was dependent on a functional p53 homologue (CEP-1), suggesting that spontaneous DNA damage accumulates due to him-6 deficiency. However, induction of germline apoptosis by ionizing radiation was not significantly affected by the deficiency, indicating that HIM-6 has no role in the induction of apoptosis by exogenous DNA damage. We conclude that the C. elegans BLM orthologue is involved in DNA repair in promeiotic cells undergoing homologous recombination, as well as in actively dividing germline and somatic cells.
Kim
Yun Mi
YM
Department of Biochemistry, College of Science, Yonsei University, Seoul 120-749, Korea.
Yang
Insil
I
Lee
Jiyeung
J
Koo
Hyeon-Sook
HS
D000251:Adenosine Triphosphatases / Q000172:deficiency*; D000483:Alleles; D000818:Animals; D017209:Apoptosis / Q000502:physiology* / Q000528:radiation effects; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000502:physiology*; D004249:DNA Damage / Q000502:physiology*; D004265:DNA Helicases / Q000172:deficiency*; D005720:Gamma Rays; D020125:Mutation, Missense; D053484:RecQ Helicases; D014466:Ultraviolet Rays
D029742:Caenorhabditis elegans Proteins; C487047:HIM-6 protein, C elegans; D000251:Adenosine Triphosphatases; C097151:Bloom syndrome protein; D004265:DNA Helicases; D053484:RecQ Helicases