title: [Effects of testis murine cytomegalovirus infection on sperm acrosome reaction and spermatic function of membrane in mice].
abstract: OBJECTIVE
  To explore the effects of testis murine cytomegalovirus (MCMV) infection on mature sperm acrosome reaction and spermatic function of membrane in mice.
  
  
  METHODS
  BALB/c mice without MCMV infection were randomly devided into two groups: an experimental group (48 mice) and a control group (30 mice). The mice in the control group were treated by inoculation DMEM without MCMV into testis, while those in the experimental one were directly inoculated with MCMV into testis. Mice in two groups were sacrificed separately at 1, 2, 4, 6, 9, 14 d post inoculation (D1, 2, 4, 6, 9, 14 PI), and the MCMV M83 mRNA gene was detected inside the testes by in situ hybridization (ISH) with one episode MCMV late-mRNA probe labeled with digoxin, meanwhile acrosome reaction and the function of membrane of mature sperms in the epididymis tails was measured.
  
  
  RESULTS
  The positive signal of ISH of MCMV was mainly founded in the two kinds of testicular cells (spermatogenic cells and Leydig cells) in the experimental group. Compared with that of the control group, the sperm acrosome reaction in the experimental group was decreased significantly by the rate from (71 +/- 6)%, (70 +/- 7)% to (58 +/- 9)%, (56 +/- 9)% (P < 0.05) separately on D2 PI and D4 PI. And the sperm membrane hypo-osmotic swelling was decreased significantly by the rate from (60 +/- 7)%, (50 +/- 4)% to (48 +/- 9)%, (38 +/- 8)% (P < 0.05) separately also on D2 PI and D4 PI.
  
  
  CONCLUSIONS
  The model of CMV infection in murine testis was established. The sperm acrosome reaction and function of membrane in mice might be descented significantly by MCMV infection in the early period, which shows that MCMV infection might influent the sperm's function.
authors.affiliation: Center of Reproductive of Medicine, Research Institute of Family Planning, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Jin-wen
authors.initials: JW
authors.lastname: Xiong
authors.forename: Cheng-liang
authors.initials: CL
authors.lastname: Xiong
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Tian
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: Hui-min
authors.initials: HM
authors.lastname: Xu
authors.forename: Lian
authors.initials: L
authors.lastname: Hu
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Ding
mesh_terms: D020101:Acrosome Reaction; D000818:Animals; D003586:Cytomegalovirus Infections / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009920:Orchitis / Q000503:physiopathology* / Q000821:virology; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*