title: [Primary study on histocompatibility of three kinds of collagen-chitosan porous scaffolds].
abstract: OBJECTIVE
  To construct three kinds of collagen-chitosan porous scaffolds with enhanced bio-stability and to investigate the histocompatibility of the scaffolds in vivo.
  
  
  METHODS
  Collagen-chitosan porous scaffolds were fabricated by freeze-drying method, cross-linked using dehydrothermal treatment and glutaraldehyde, respectively. The morphology of the uncross-linked scaffold (scaffold 1), dehydrothermal cross-linked scaffold (scaffold 2) and glutaraldehyde cross-linked scaffold (scaffold 3) was studied by scanning electron microscopy. Three kinds of scaffolds were embedded subcutaneously on dorsal surface of 12 rabbit ears. The general and local responses were recorded daily. The bio-stability and histocompatibility of the scaffolds were observed by using HE staining after 3, 7, 14 and 28 days of operation.
  
  
  RESULTS
  The scaffolds had three-dimensional porous structures with a porosity of more than 90%, and possessed pore sizes of 120 +/- 10 microm, 80 +/- 15 microm and 170 +/- 20 microm, respectively. All experimental rabbits survived with good general condition during the study. All skin incisions healed well without obvious reactive red or swelling. Histological study showed that scaffold 1 was degraded rapidly with obvious inflammation. The degradation of scaffold 2 was slower than that of scaffold 1 and the inflammation of scaffold 2 was also milder than that of scaffold 1. Scaffold 3 possessed slow degradation property with slight inflammatory reaction, and rapid tissue regeneration.
  
  
  CONCLUSIONS
  The collagen-chitosan porous scaffolds have three-dimensional porous structures that are suitable for tissue regeneration. The bio-stability and histocompatibility of the scaffolds are enhanced after cross-linked. Glutaraldehyde cross-linked is better than dehydrothermal cross-linked, which can facilitate dermal tissue reconstruction.
authors.affiliation: Department of Burn, Second Affiliated Hospital of Zhejiang University, Hangzhou Zhejiang, 310009, PR China.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Hu
authors.forename: Chunmao
authors.initials: C
authors.lastname: Han
authors.forename: Haifei
authors.initials: H
authors.lastname: Shi
authors.forename: Lie
authors.initials: L
authors.lastname: Ma
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000493:pharmacokinetics; D048271:Chitosan / Q000737:chemistry / Q000493:pharmacokinetics; D003094:Collagen / Q000737:chemistry / Q000493:pharmacokinetics; D005260:Female; D008297:Male; D008422:Materials Testing; D023421:Models, Animal; D011817:Rabbits; D011897:Random Allocation; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003094:Collagen; D048271:Chitosan