Trp-262 is a key residue for the hydrolytic and transglucosidic reactivity of the Aspergillus niger family 3 beta-glucosidase: substitution results in enzymes with mainly transglucosidic activity.
Trp-262 of the Aspergillus niger family 3 beta-glucosidase is shown in this report to be a key residue for determining the ratio of this enzyme's hydrolytic and transglucosidic activities. TLC showed that when cellobiose was both the substrate and the acceptor, beta-glucosidases with substitutions (Phe, Ala, Leu, and Cys) for Trp-262 formed very high amounts of transglucosidic adducts. When pNPGlc was the substrate and the acceptor of the substituted beta-glucosidases, only transglucosidic adducts and pNP were produced. Little or no Glc could be detected, indicating that the reactions occurring were mainly transglucosidic. GLC studies with cellobiose quantitatively showed that one Glc was transferred for each free Glc produced. Since this is the maximum level of transglucosidation possible, this again showed that the reaction is predominantly transglucosidic. Analyses of the K(m) and K(i) values of cello-oligosaccharides of increasing length, of the K(i) values of Glc and of the transglucosidic activity at low acceptor concentration, showed that substitution for Trp-262 causes poor binding at the binding site for the non-reducing Glc of the substrate while the affinity for other Glc units is only minimally affected. The acceptor sites become saturated with substrate (acceptor) at the concentrations needed for glucosidic bond cleavage and thus only transglucosidic reactions occur. In addition, the data indicate that substitution for Trp-262 causes the rate of the hydrolysis step (k(3)) to be small.
Seidle
Heather F
HF
Division of Biochemistry, Faculty of Science, University of Calgary, 2500 University Dr. NW, Calgary, Alta., Canada T2N 1N4.
McKenzie
Kyle
K
Marten
Ira
I
Shoseyov
Oded
O
Huber
Reuben E
RE
D019943:Amino Acid Substitution; D001234:Aspergillus niger / Q000201:enzymology*; D001483:Base Sequence; D001665:Binding Sites; D002475:Cellobiose / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D014364:Tryptophan / Q000737:chemistry* / Q000235:genetics; D001617:beta-Glucosidase / Q000737:chemistry* / Q000235:genetics
D002475:Cellobiose; D014364:Tryptophan; D001617:beta-Glucosidase