title: Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei.
abstract: A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.
authors.affiliation: Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640, USA.
authors.forename: Jana M
authors.initials: JM
authors.lastname: U'Ren
authors.forename: Matthew N
authors.initials: MN
authors.lastname: Van Ert
authors.forename: James M
authors.initials: JM
authors.lastname: Schupp
authors.forename: W Ryan
authors.initials: WR
authors.lastname: Easterday
authors.forename: Tatum S
authors.initials: TS
authors.lastname: Simonson
authors.forename: Richard T
authors.initials: RT
authors.lastname: Okinaka
authors.forename: Talima
authors.initials: T
authors.lastname: Pearson
authors.forename: Paul
authors.initials: P
authors.lastname: Keim
mesh_terms: D001483:Base Sequence; D042726:Burkholderia mallei / Q000145:classification* / Q000235:genetics; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics; D017931:DNA Primers; D005798:Genes, Bacterial / Q000235:genetics; D005843:Geography; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D013045:Species Specificity; D019914:Taq Polymerase
chemical_list: D017931:DNA Primers; D009711:Nucleotides; D019914:Taq Polymerase