Induction and expression of GST-Pi foci in the liver of Cyclophosphamide-administered rats.
Anticancer drug Cyclophosphamide (CY) is metabolized to phosphoramide mustard and acrolein by the hepatic P450 enzymes. GST-Pi is a biochemical feature which occurs in carcinogen induced preneoplastic foci and it plays an important role in the detoxification pathway of acrolein metabolism. Administration of CY induces GST-Pi positive single cells and foci expression in rat liver and these can be considered as precursors of preneoplastic foci leading to hepatocarcinogenesis. The expression of GST-Pi in CY-treated rats on different days of treatment was confirmed by immunohistochemistry, immunoblot, RT-PCR and by ELISA. We also advocate that epigenetic mechanism could be accounted for the GST-Pi induction in the hepatocytes of CY-treated rats.
Devi
Arikketh
A
Unit of Biochemistry, Department of Zoology, University of Madras, Life Sciences Building, Guindy Campus, Chennai 600025, Tamil Nadu, India.
Devaraj
Halagowder
H
D000171:Acrolein / Q000378:metabolism; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000378:metabolism / Q000494:pharmacology; D003520:Cyclophosphamide / Q000378:metabolism / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D051549:Glutathione S-Transferase pi / Q000235:genetics* / Q000378:metabolism*; D015151:Immunoblotting; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008297:Male; D012313:RNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D018906:Antineoplastic Agents, Alkylating; D012313:RNA; D000171:Acrolein; D003520:Cyclophosphamide; D051549:Glutathione S-Transferase pi