Production of pectinase from deseeded sunflower head by Aspergillus niger in submerged and solid-state conditions.
Studies were carried out on the production of pectinases using deseeded sunflower head by Aspergillus niger DMF 27 and DMF 45 in submerged fermentation (SmF) and solid-state fermentation (SSF). Higher titres of endo- and exo-pectinases were observed when medium was supplemented with carbon (4% glucose for SmF and 6% sucrose for SSF) and nitrogen (ammonium sulphate, 0.3% for both SmF and SSF) sources. Green gram husk proved to be relatively a better supplement to attain higher yield of endo-pectinase (11.7 U/g) and exo-pectinase (30.0 U/g) in solid-state conditions. Maximum production of endo-pectinase (19.8 U/g) and exo-pectinase (45.9 U/g) by DMF 45 were recorded in SSF when compared to endo-pectinase (18.9 U/ml) and exo-pectinase (30.3 U/ml) by DMF 27 in SmF under optimum process conditions.
Patil
Sarvamangala R
SR
Department of Microbiology, Vishweshwariah College of Applied Sciences, Gulbarga 585102, India. smrpatil@rediffmail.com
Dayanand
A
A
D001234:Aspergillus niger / Q000378:metabolism*; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D005285:Fermentation; D035263:Flowering Tops / Q000378:metabolism / Q000382:microbiology*; D006368:Helianthus; D007194:India; D009584:Nitrogen / Q000378:metabolism; D011096:Polygalacturonase / Q000096:biosynthesis*
D002244:Carbon; D011096:Polygalacturonase; D009584:Nitrogen