Analysis of putative RNase P RNA from orthopoxviruses.
A putative RNase P RNA gene in camelpox virus, one of the orthopoxviruses, was cloned and transcribed in vitro. No RNase P activity could be detected in vitro from camelpox virus RNase P RNA alone, or by addition of the Escherichia coli RNase P protein subunit to reaction mixtures. Camelpox virus RNase P RNA reconstituted in vitro with camel or HeLa cell extracts, which were pre-treated with micrococcal nuclease to degrade the endogenous RNase P RNA, showed no RNase P activity. Vaccinia virus, another orthopoxvirus, showed no RNase P activity in vaccinia-infected HeLa cells, even though transcription of the vaccinia RNase P RNA could be identified in the cells by both Northern blot and RNase protection assay. Camelpox virus RNase P RNA inhibited an endogenous HeLa RNase P activity by 20% in our assays. The 5S RNA showed no significant inhibition in this assay.
Yang
Li
L
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Wesolowski
Donna
D
Li
Yong
Y
Altman
Sidney
S
D000818:Animals; D001483:Base Sequence; D002162:Camelus; D004926:Escherichia coli / Q000201:enzymology; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D018155:Orthopoxvirus / Q000201:enzymology* / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D043262:Ribonuclease P / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics
D012341:RNA, Ribosomal, 5S; D012367:RNA, Viral; D043262:Ribonuclease P