title: Preterm birth in twins after subfertility treatment: population based cohort study.
abstract: OBJECTIVE
  To assess gestational length and prevalence of preterm birth among medically and naturally conceived twins; to establish the role of zygosity and chorionicity in assessing gestational length in twins born after subfertility treatment.
  
  
  METHODS
  Population based cohort study.
  
  
  METHODS
  Collaborative network of 19 maternity facilities in East Flanders, Belgium (East Flanders prospective twin survey).
  
  
  METHODS
  4368 twin pairs born between 1976 and 2002, including 2915 spontaneous twin pairs, 710 twin pairs born after ovarian stimulation, and 743 twin pairs born after in vitro fertilisation or intracytoplasmic sperm injection.
  
  
  METHODS
  Gestational length and prevalence of preterm birth.
  
  
  RESULTS
  Compared with naturally conceived twins, twins resulting from subfertility treatment had on average a slightly decreased gestational age at birth (mean difference 4.0 days, 95% confidence interval 2.7 to 5.2), corresponding to an odds ratio of 1.6 (1.4 to 1.8) for preterm birth, albeit confined to mild preterm birth (34-36 weeks). The adjusted odds ratios of preterm birth after subfertility treatment were 1.3 (1.1 to 1.5) when controlled for birth year, maternal age, and parity and 1.6 (1.3 to 1.8) with additional control for fetal sex, caesarean section, zygosity, and chorionicity. Although an increased risk of preterm birth was therefore seen among twins resulting from subfertility treatment, the risk was largely caused by a first birth effect among subfertile couples; conversely, the risk of prematurity was substantially levelled off by the protective effect of dizygotic twinning.
  
  
  CONCLUSIONS
  Twins resulting from subfertility treatment have an increased risk of preterm birth, but the risk is limited to mild preterm birth, primarily by virtue of dizygotic twinning.
authors.affiliation: Department of Obstetrics and Gynaecology, Ghent University Hospital 1P3, De Pintelaan, B-9000 Ghent, Belgium. hans.verstraelen@UGent.be
authors.forename: Hans
authors.initials: H
authors.lastname: Verstraelen
authors.forename: Sylvie
authors.initials: S
authors.lastname: Goetgeluk
authors.forename: Catherine
authors.initials: C
authors.lastname: Derom
authors.forename: Stijn
authors.initials: S
authors.lastname: Vansteelandt
authors.forename: Robert
authors.initials: R
authors.lastname: Derom
authors.forename: Els
authors.initials: E
authors.lastname: Goetghebeur
authors.forename: Marleen
authors.initials: M
authors.lastname: Temmerman
mesh_terms: D001724:Birth Weight; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D008423:Maternal Age; D010298:Parity; D011247:Pregnancy; D011272:Pregnancy, Multiple / Q000706:statistics & numerical data*; D047928:Premature Birth / Q000209:etiology*; D011446:Prospective Studies; D027724:Reproductive Techniques, Assisted / Q000009:adverse effects* / Q000706:statistics & numerical data; D012307:Risk Factors; D014429:Twins, Dizygotic; D014430:Twins, Monozygotic