Non-culture-based analysis of bacterial populations from patients with chronic rhinosinusitis.
Middle meatus aspirates from patients with chronic rhinosinusitis were analyzed by bacterial culture, denaturing gradient gel electrophoresis (DGGE), and antibiotic sensitivity techniques. DGGE detected a greater bacterial diversity than culture methods. Although resistance to antibiotics was low, there was evidence of changes in the composition of the bacterial microbiota over time, and the presence of noncultured bacteria was demonstrated.
Power
Daniel A
DA
Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
Burton
Jeremy P
JP
Chilcott
Chris N
CN
Tagg
John R
JR
Dawes
Patrick J
PJ
D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D002908:Chronic Disease; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012220:Rhinitis / Q000382:microbiology*; D012852:Sinusitis / Q000382:microbiology*
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S