title: Novel scavenger receptor gene is differentially expressed in the embryonic and adult mouse testis.
abstract: In an effort to understand the mechanisms that underpin gonadal differentiation at the time of sex determination, we identified a cDNA encoding a putative novel testis expressed scavenger receptor, Tesr. Based on its domain structure, we hypothesize that the function of Tesr is similar to that of other scavenger receptors that play roles in phagocytosis of apoptotic cells, cell-cell adhesion, and defense. Tesr mRNA was detected in fetal mouse gonads of both sexes at 11.5 days post coitum (dpc). From 12.0 dpc, Tesr expression rapidly decreased in the female and was maintained in the male. Expression was seen in embryonic mouse sites other than the testis, such as in brain, eye, head, heart, neural arch, and cartilage primordium. Tesr expression in the newborn testis was faint to undetectable, but it increased from 2 days postpartum (dpp) until 15 dpp and was found in a subset of interstitial cells and in germ and Sertoli cells. Tesr mRNA in the adult mouse testis was observed in Sertoli cells, spermatogonia, spermatocytes, round spermatids, and in a subset of interstitial cells. We conclude that Tesr is differentially expressed in the male vs. female embryonic gonad and is expressed in both the ovary and the testes postnatally after 2 dpp.
authors.affiliation: The ARC Centre of Excellence in Biotechnology and Development, Melbourne, VIC, Australia. mai.sarraj@med.monash.edu.au
authors.forename: M A
authors.initials: MA
authors.lastname: Sarraj
authors.forename: P J
authors.initials: PJ
authors.lastname: McClive
authors.forename: H P
authors.initials: HP
authors.lastname: Wilmore
authors.forename: K L
authors.initials: KL
authors.lastname: Loveland
authors.forename: A H
authors.initials: AH
authors.lastname: Sinclair
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D017931:DNA Primers; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051116:Receptors, Scavenger / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012733:Sex Differentiation / Q000235:genetics*; D012737:Sex Factors; D013737:Testis / Q000196:embryology* / Q000378:metabolism*; D051117:Scavenger Receptors, Class A
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D051116:Receptors, Scavenger; C511405:SCARA5 protein, mouse; D051117:Scavenger Receptors, Class A