title: A novel apoptosis-inducing monoclonal antibody (anti-LHK) against a cell surface antigen on colon cancer cells.
abstract: BACKGROUND
  Apoptosis is a crucial element in the behavior of mammalian cells in many different situations. We here report the establishment of a novel monoclonal antibody (anti-LHK mAb) that has apoptosis-inducing activity against colon cancer Colo205 cells.
  
  
  METHODS
  The mechanism of anti-LHK mAb-induced cell death was assessed by microscopic morphology, Annexin V/Hoechst 33528 staining, and detection of DNA fragmentation. The molecular weight of LHK antigen was determined by Western blotting. Growth inhibition of Colo205 cells induced by anti-LHK mAb was determined by in vitro and in vivo studies.
  
  
  RESULTS
  Anti-LHK reacted with a 70-kDa antigen and completely blocked the proliferation of Colo205 cells bearing LHK in vitro in a manner characteristic of apoptosis. Strikingly, anti-LHK mAb suppressed tumor growth in a murine peritoneal dissemination model.
  
  
  CONCLUSIONS
  LHK antigen, which is restricted to epithelial cells, may be a novel death receptor that plays a critical role in controlling the growth, invasion, and metastasis of human colon cancer cells.
authors.affiliation: Department of Internal Medicine, School of Medicine, Keio University, Tokyo, 160-8582, Japan.
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Matsukawa
authors.forename: Takanori
authors.initials: T
authors.lastname: Kanai
authors.forename: Makoto
authors.initials: M
authors.lastname: Naganuma
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Kamada
authors.forename: Tadakazu
authors.initials: T
authors.lastname: Hisamatsu
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Takaishi
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Ogata
authors.forename: Makio
authors.initials: M
authors.lastname: Mukai
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Ishii
authors.forename: Tsuneyo
authors.initials: T
authors.lastname: Mimori
authors.forename: Mamoru
authors.initials: M
authors.lastname: Watanabe
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Hibi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface; D017209:Apoptosis / Q000276:immunology*; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000276:immunology* / Q000473:pathology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface