title: Simple sequence repeat-based consensus linkage map of Bombyx mori.
abstract: We established a genetic linkage map employing 518 simple sequence repeat (SSR, or microsatellite) markers for Bombyx mori (silkworm), the economically and culturally important lepidopteran insect, as part of an international genomics program. A survey of six representative silkworm strains using 2,500 (CA)n- and (CT)n-based SSR markers revealed 17-24% polymorphism, indicating a high degree of homozygosity resulting from a long history of inbreeding. Twenty-nine SSR linkage groups were established in well characterized Dazao and C108 strains based on genotyping of 189 backcross progeny derived from an F(1) male mated with a C108 female. The clustering was further focused to 28 groups by genotyping 22 backcross progeny derived from an F(1) female mated with a C108 male. This set of SSR linkage groups was further assigned to the 28 chromosomes (established linkage groups) of silkworm aided by visible mutations and cleaved amplified polymorphic sequence markers developed from previously mapped genes, cDNA sequences, and cloned random amplified polymorphic DNAs. By integrating a visible mutation p (plain, larval marking) and 29 well conserved genes of insects onto this SSR-based linkage map, a second generation consensus silkworm genetic map with a range of 7-40 markers per linkage group and a total map length of approximately 3431.9 cM was constructed and its high efficiency for genotyping and potential application for synteny studies of Lepidoptera and other insects was demonstrated.
authors.affiliation: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.
authors.forename: Xue-Xia
authors.initials: XX
authors.lastname: Miao
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Xub
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Li
authors.forename: Mu-Wang
authors.initials: MW
authors.lastname: Li
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Huang
authors.forename: Fang-Yin
authors.initials: FY
authors.lastname: Dai
authors.forename: Susan W
authors.initials: SW
authors.lastname: Marino
authors.forename: David R
authors.initials: DR
authors.lastname: Mills
authors.forename: Peiyu
authors.initials: P
authors.lastname: Zeng
authors.forename: Kazuei
authors.initials: K
authors.lastname: Mita
authors.forename: Shi-Hai
authors.initials: SH
authors.lastname: Jia
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Xiang
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Guo
authors.forename: An-Ying
authors.initials: AY
authors.lastname: Xu
authors.forename: Xiang-Yin
authors.initials: XY
authors.lastname: Kong
authors.forename: Hong-Xuan
authors.initials: HX
authors.lastname: Lin
authors.forename: Yao-Zhou
authors.initials: YZ
authors.lastname: Shi
authors.forename: Gang
authors.initials: G
authors.lastname: Lu
authors.forename: Xianglin
authors.initials: X
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yasukochi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sugasaki
authors.forename: Toru
authors.initials: T
authors.lastname: Shimada
authors.forename: Javaregowda
authors.initials: J
authors.lastname: Nagaraju
authors.forename: Zhong-Huai
authors.initials: ZH
authors.lastname: Xiang
authors.forename: Sheng-Yue
authors.initials: SY
authors.lastname: Wang
authors.forename: Marian R
authors.initials: MR
authors.lastname: Goldsmith
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zhao
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Huang
mesh_terms: D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D002874:Chromosome Mapping; D008040:Genetic Linkage; D005819:Genetic Markers; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D005819:Genetic Markers