Adenoviral-mediated gene delivery to trophoblast cells.
This chapter focuses on technology for construction of recombinant adenoviruses containing reporter genes under the control of putative regulatory regions of the human (h)CYP19 (aromatase) gene, as well as expression vectors. These recombinant adenoviruses have been used in primary cultures of human placental cells to characterize regulatory regions of the hCYP19 gene and to analyze the function of transcription factors on hCYP19 expression and on trophoblast differentiation.
Jiang
Bing
B
Department of Obstetrics & Gynecology, University of Texas Southwestern Medical Center, Dallas, USA.
Mendelson
Carole R
CR
D000256:Adenoviridae / Q000235:genetics*; D001141:Aromatase / Q000235:genetics; D002478:Cells, Cultured; D004274:DNA, Recombinant / Q000235:genetics; D005260:Female; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011247:Pregnancy; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D014162:Transfection / Q000379:methods*; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism*
D004274:DNA, Recombinant; D001141:Aromatase