Potential ecotoxic effects of polychlorinated biphenyls on Xenopus laevis.
We examined potential ecotoxic effects of polychlorinated biphenyl (PCB)3, PCB5, Aroclor 1254, and Aroclor 1242 on Xenopus laevis. Tadpoles were exposed to PCBs from stage 46/47 (system of Nieuwkoop and Faber) to the completion of metamorphosis. We demonstrated, to our knowledge for the first time, forelimb malformations caused by PCBs (malformation rate, > 70%). The malformed forelimbs were fixed in the adduction-backward rotation position and could not move. Therefore, malformed male frogs were destined to have no offspring, because they could not grasp the females with their forelimbs to mate. Alcian blue-alizarin red double-staining indicated that the forelimb malformation resulted from the shoulder abnormality. Compared with the normal shoulder joint, the proximal humerus with the humerus inter-rotated 90 degrees in the abnormal shoulder joint. Moreover, testes from more than a third of male frogs with exposed to PCBs exhibited feminization to different degrees at gross morphology and histology, with fewer or abnormal spermatogonia and oocytes. Gonadal abnormalities would lead directly to reproductive dysfunction and population decline. These results suggest that PCBs have potentially ecotoxic effects on amphibian populations. We infer that PCBs could play roles in amphibian malformations and population declines, at least at sites that are polluted heavily with PCBs.
Qin
Zhan-Fen
ZF
The State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Zhou
Jing-Ming
JM
Cong
Lin
L
Xu
Xiao-Bai
XB
D000818:Animals; D000013:Congenital Abnormalities / Q000209:etiology* / Q000662:veterinary; D005260:Female; D005552:Forelimb / Q000002:abnormalities; D006066:Gonads / Q000002:abnormalities; D007814:Larva / Q000254:growth & development; D008297:Male; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011157:Population Dynamics; D012098:Reproduction; D018570:Risk Assessment; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014982:Xenopus laevis
D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls