title: Time-course of radiation-induced chromosomal aberrations in tumor patients after radiotherapy.
abstract: OBJECTIVE
  Radiation-induced chromosome aberrations are routinely used in biologic dosimetry to monitor radiation exposure. Translocations are considered stable aberrations with time after exposure. This study was performed to determine the temporal persistence of radiation-induced translocations during a 36-month period in therapeutically irradiated testicular seminoma patients who underwent partial body exposure (>10% of bone marrow).
  
  
  METHODS
  Chromosome analyses were carried out in peripheral lymphocytes of 11 patients with testicular seminoma (n = 9), germinoma (n = 1), or follicular non-Hodgkin's lymphoma (n = 1). All patients received radiotherapy with photons from a linear accelerator; in 1 case, additional electron beams were used. Doses ranged from 26 Gy (seminoma) to 45 Gy (non-Hodgkin's lymphoma). None of the patients received chemotherapy. From each patient, blood samples were taken during the 36 months after irradiation at defined points. Chromosomal aberrations were scored after fluorescence in situ hybridization painting of chromosomes 1, 4, and 12 in combination with a pancentromeric probe.
  
  
  RESULTS
  For 9 patients (7 with testicular seminoma, 1 with germinoma, and 1 with non-Hodgkin's lymphoma), a significant temporal decline of translocations, with a mean decline rate of 4.4% +/- 0.4% monthly, could be detected. Two testicular seminoma patients showed no temporal decline of aberration frequencies.
  
  
  CONCLUSIONS
  Most partial body irradiated patients (9 of 11) showed a significant temporal decline of translocation frequencies during a 36-month period. Thus, reciprocal translocations after partial body irradiation cannot be regarded as stable over time. The temporal decline of aberration frequencies has to be taken into account for retrospective dose estimations.
authors.affiliation: GSF-National Research Center for Environment and Health, GmBH, Institute of Molecular Radiation Biology, Neuherberg, Germany.
authors.forename: Irene
authors.initials: I
authors.lastname: Müller
authors.forename: Hans
authors.initials: H
authors.lastname: Geinitz
authors.forename: Herbert
authors.initials: H
authors.lastname: Braselmann
authors.forename: Adolf
authors.initials: A
authors.lastname: Baumgartner
authors.forename: Annette
authors.initials: A
authors.lastname: Fasan
authors.forename: Reinhard
authors.initials: R
authors.lastname: Thamm
authors.forename: Michael
authors.initials: M
authors.lastname: Molls
authors.forename: Viktor
authors.initials: V
authors.lastname: Meineke
authors.forename: Horst
authors.initials: H
authors.lastname: Zitzelsberger
mesh_terms: D000328:Adult; D000368:Aged; D018237:Germinoma / Q000235:genetics / Q000532:radiotherapy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D018239:Seminoma / Q000235:genetics / Q000532:radiotherapy; D013736:Testicular Neoplasms / Q000235:genetics / Q000532:radiotherapy; D013997:Time Factors; D014178:Translocation, Genetic