Endosperm-preferred expression of maize genes as revealed by transcriptome-wide analysis of expressed sequence tags.
The transcriptome-wide endosperm-preferred expression of maize genes was addressed by analyzing a large database of expressed sequence tags (ESTs). We generated 30,531 high quality sequence-reads from the 5'-ends of cDNA libraries from maize endosperm harvested at 10, 15, and 20 days after pollination. A further 196,900 maize sequence-reads retrieved from public databases were added to this endosperm collection to generate MAIZEST, a database with tools for data storage and analysis. MAIZEST contains 227,431 ESTs, one third of which represents developing endosperm and the remaining two-thirds represent transcripts from 49 cDNA libraries constructed from different organs and tissues. Assembling the MAIZEST ESTs generated 29,206 putative transcripts, of which a set of 4032 assembled sequences was composed exclusively of sequences derived from endosperm cDNA libraries. After sequence analysis using overlapping parameters, a sub-set of 2403 assembled sequences was functionally annotated and revealed a wide variety of putative new genes involved in endosperm development and metabolism.
Verza
Natalia C
NC
Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil.
E Silva
Thaís Rezende
TR
Neto
Germano Cord
GC
Nogueira
Fábio T S
FT
Fisch
Paulo H
PH
de Rosa
Vincente E
VE
Rebello
Marcelo M
MM
Vettore
André L
AL
da Silva
Felipe Rodrigues
FR
Arruda
Paulo
P
D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development*
D010940:Plant Proteins