title: Platelet function and association of bovine viral diarrhea virus with platelets of persistently infected cattle.
abstract: OBJECTIVE
  To determine whether viral involvement with platelets obtained from cattle persistently infected (PI) with bovine viral diarrhea virus (BVDV) is associated with altered platelet function or decreased platelet counts.
  
  
  METHODS
  Platelets obtained from 8 cattle PI with BVDV and 6 age-, sex-, and breed-matched uninfected control cattle.
  
  
  METHODS
  Manual platelet counts were determined, and platelet function was assessed through optical aggregometry by use of the aggregation agonists ADP and platelet-activating factor. Identification of BVDV in serum and preparations of purified platelets was determined by use of virus isolation tests.
  
  
  RESULTS
  No significant difference in platelet counts was detected between cattle PI with BVDV and control cattle. In response to the aggregation agonists, maximum aggregation percentage and slope of the aggregation curve were not significantly different between cattle PI with BVDV and control cattle. We isolated BVDV from serum of all PI cattle and from purified platelets of 6 of 8 PI cattle, but BVDV was not isolated from serum or platelets of control cattle.
  
  
  CONCLUSIONS
  Isolation of BVDV from platelets in the peripheral circulation of cattle immunotolerant to BVDV does not result in altered platelet function or decreases in platelet counts.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL 36849-5519, USA.
authors.forename: Paul H
authors.initials: PH
authors.lastname: Walz
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Grooms
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Bell
authors.forename: Lana
authors.initials: L
authors.lastname: Kaiser
authors.forename: John C
authors.initials: JC
authors.lastname: Baker
authors.forename: Kenny V
authors.initials: KV
authors.lastname: Brock
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001792:Blood Platelets / Q000821:virology*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000097:blood*; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000302:isolation & purification* / Q000378:metabolism; D030242:Diarrhea Virus 2, Bovine Viral / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D008297:Male; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology
chemical_list: D010972:Platelet Activating Factor