title: Evaluation of alginate purification methods: effect on polyphenol, endotoxin, and protein contamination.
abstract: Alginate, a polysaccharide extracted from brown seaweed, is widely used for the microencapsulation of islets of Langerhans, allowing their transplantation without immunosuppression. This natural polymer is known to be largely contaminated. The implantation of islets encapsulated using unpurified alginate leads to the development of fibrotic cell overgrowth around the microcapsules and normalization of the blood glucose is restricted to a very short period if it is achieved at all. Several research groups have developed their own purification method and obtained relatively good results. No comparative evaluation of the efficiencies of these methods has been published. We conducted an evaluative study of five different alginate preparations: a pharmaceutical-grade alginate in its raw state, the same alginate after purification according to three different published methods, and a commercially available purified alginate. The results showed that all purification methods reduced the amounts of known contaminants, that is, polyphenols, endotoxins, and proteins, although with varying efficiencies. Increased viscosity of alginate solutions was observed after purification of the alginates. Despite a general efficiency in decreasing contamination levels, all of the purified alginates contained relatively high residual amounts of protein contaminants. Because proteins may be immunogenic, these residual proteins may have a role in persisting microcapsule immunogenicity.
authors.affiliation: Centre de recherche Guy-Bernier, Hôpital Maisonneuve-Rosemont, Montréal, Québec, Canada H1T 2M4.
authors.forename: Julie
authors.initials: J
authors.lastname: Dusseault
authors.forename: Susan K
authors.initials: SK
authors.lastname: Tam
authors.forename: Martin
authors.initials: M
authors.lastname: Ménard
authors.forename: Stefania
authors.initials: S
authors.lastname: Polizu
authors.forename: Guillaume
authors.initials: G
authors.lastname: Jourdan
authors.forename: L'Hocine
authors.initials: L
authors.lastname: Yahia
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Hallé
mesh_terms: D000464:Alginates / Q000302:isolation & purification*; D005591:Chemical Fractionation; D004339:Drug Compounding / Q000592:standards; D004340:Drug Contamination / Q000517:prevention & control*; D004731:Endotoxins / Q000302:isolation & purification; D005419:Flavonoids / Q000302:isolation & purification; D006801:Humans; D016381:Islets of Langerhans Transplantation; D008422:Materials Testing; D010636:Phenols / Q000302:isolation & purification; D059808:Polyphenols; D011506:Proteins / Q000302:isolation & purification; D014783:Viscosity
chemical_list: D000464:Alginates; D004731:Endotoxins; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D011506:Proteins