Enrichment and isolation of iron-oxidizing bacteria at neutral pH.
Methods are provided for the culture of neutrophilic Fe-oxidizing bacteria (FeOB) that grow under microaerobic and anaerobic conditions. For oxygen-requiring lithotrophic Fe oxidizers, it is essential that both O2 and Fe(II) concentrations are kept low, but that an adequate flux of both O2 and Fe(II) are provided to support growth. Techniques using opposing gradients of Fe(II) and O2 are discussed. Preparation of stock solutions of FeS and FeCl2 are described. Methods for the culture of anaerobic FeOB that utilize nitrate or light (anoxygenic photosynthesis) are discussed and presented briefly.
Emerson
David
D
American Type Culture Collection, Manassas, Virginia 20110, USA.
Floyd
Melissa Merrill
MM
D001421:Bacteria, Anaerobic / Q000302:isolation & purification* / Q000378:metabolism; D001431:Bacteriological Techniques; D020562:Betaproteobacteria / Q000302:isolation & purification / Q000378:metabolism; D003470:Culture Media; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction
D003470:Culture Media; D005296:Ferrous Compounds; D009566:Nitrates; D007501:Iron; C022597:ferrous sulfide