The effect of a single boranophosphate substitution with defined configuration on the thermal stability and conformation of a DNA duplex.
Substitution of one non-bridging oxygen in a natural phosphodiester internucleotide linkage with a borano (-BH3) group results in a chiral phosphorus center in boranophosphate. UV thermal melting profiles were recorded for DNA duplexes formed between a DNA 9-mer with either an Sp or a Rp boranophosphate linkage in the middle and the complementary DNA 9-mer, as well as for their unmodified parent duplex. The thermal stability of the DNA duplexes was in the order of normal > Sp borano > Rp borano. CD spectra of all three duplexes exhibited typical B-DNA profile, which closely resembled each other.
Wang
Joy Xin
JX
Chemistry, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina, USA.
Sergueev
Dmitri S
DS
Shaw
Barbara Ramsay
BR
D001880:Boranes / Q000737:chemistry*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D006358:Hot Temperature; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D013053:Spectrophotometry; D013237:Stereoisomerism; D013696:Temperature; D014466:Ultraviolet Rays
D001880:Boranes; D018076:DNA, Complementary; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; D010710:Phosphates; D012313:RNA; D004247:DNA