Desialylation decreases the resistance of apo B-containing lipoproteins to aggregation and increases their atherogenic potential.
Subfractions of apo B-containing lipoproteins (VLDL and intermediate-density lipoproteins) with reduced content of sialic acid were found in human blood. These lipoproteins are characterized by high capacity to spontaneous association (aggregation) and stimulated accumulation of cholesterol in smooth muscle cells of human aortic intima. In vitro treatment of apo B-containing lipoproteins with alpha-2,6-sialidase and alpha-2,3-sialidase stimulated aggregation and increased the ability of these particles to potentiate cholesterol accumulation in smooth muscle cells of the intact human aortic intima. Probably, desialylation of various apo B-containing lipoproteins can occur in the blood; this process decreases their resistance to aggregation, and increases the ability of these particles to stimulate accumulation of cholesterol in human aortic intima cells, i.e. increases their atherogenic potential.
Mel'nichenko
A A
AA
Laboratory of Physicochemical Methods for Study and Analysis, Institute of Physicochemical Medicine, Russian Ministry of Health, Moscow. inat-science@yandex.ru
Tertov
V V
VV
Ivanova
O A
OA
Aksenov
D V
DV
Sobenin
I A
IA
Popov
E V
EV
Kaplun
V V
VV
Suprun
I V
IV
Panasenko
O M
OM
Orekhov
A N
AN
D000704:Analysis of Variance; D001055:Apolipoproteins B / Q000378:metabolism*; D050197:Atherosclerosis / Q000139:chemically induced / Q000378:metabolism*; D006801:Humans; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism*; D053419:Lipoproteins, IDL; D008079:Lipoproteins, VLDL / Q000097:blood / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009439:Neuraminidase / Q000633:toxicity*; D009522:Newcastle disease virus / Q000737:chemistry
D001055:Apolipoproteins B; D008074:Lipoproteins; D053419:Lipoproteins, IDL; D008079:Lipoproteins, VLDL; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid