Microbial conversion of major ginsenoside rb(1) to pharmaceutically active minor ginsenoside rd.
More than seventy strains of aerobic bacteria showing beta-glucosidase activity were isolated from a ginseng field, using a newly designed Esculin-R2A agar, and identified by their 16S rRNA gene sequences. Of these microorganisms, twelve strains could convert the major ginsenoside, Rb(1), to the pharmaceutically active minor ginsenoside Rd. Three strains, Burkholderia pyrrocinia GP16, Bacillus megaterium GP27 and Sphingomonas echinoides GP50, were phylogenetically studied, and observed to be most potent at converting ginsenoside Rb(1) almost completely within 48 h, as shown by TLC and HPLC analyses.
Kim
Myung Kyum
MK
Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University, Yongin, Kyunggi-do 449-701, Republic of Korea.
Lee
Jun Won
JW
Lee
Ki Young
KY
Yang
Deok-Chun
DC
D000362:Agar; D001410:Bacillus megaterium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019117:Burkholderia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media; D004929:Esculin; D036145:Ginsenosides / Q000378:metabolism*; D005894:Panax / Q000382:microbiology*; D010802:Phylogeny; D020578:Sphingomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
D003470:Culture Media; D036145:Ginsenosides; D004929:Esculin; D000362:Agar; C440192:ginsenoside-beta-glucosidase; D001617:beta-Glucosidase