title: Receptor-binding cancer antigen expressed on SiSo cells can be detected in metastatic lymph nodes from gastrointestinal cancers.
abstract: OBJECTIVE
  To investigate the expression of receptor-binding cancer antigen expressed on SiSo cells (RCAS1) in metastatic lymph nodes from gastrointestinal cancer.
  
  
  METHODS
  Metastatic lymph nodes from gastrointestinal cancer were detected for RCAS1 by immunohistochemical staining and mRNA in situ hybridization.
  
  
  RESULTS
  A total of 102 metastatic lymph nodes from bile duct, gastric, colon and pancreatic cancer were investigated for RCAS1 expression. The immunoreactivity of RCAS1 was identified in 100% of metastatic lymph nodes. Both local and distant metastatic lymph nodes showed RCAS1 expression. On the contrary, specimens of non-cancerous lymph nodes were negative for RCAS1. The result of mRNA in situ hybridization was also confirmed by the finding of immunohistochemical staining. RCAS1 mRNA was detected in all tumor cells that metastasized to lymph nodes.
  
  
  CONCLUSIONS
  All metastatic lymph nodes express RCAS1 in tumor cells at both mRNA levels, and RCAS1 that should be used as a complementary factor for identification of metastatic lymph nodes from gastrointestinal cancers.
authors.affiliation: Department of Surgery, Rajavithi Hospital, Rajavithi Road, Rajathevi, Bangkok 10400, Thailand. kawin@tubtim.cri.or.th
authors.forename: Kawin
authors.initials: K
authors.lastname: Leelawat
authors.forename: Surang
authors.initials: S
authors.lastname: Engprasert
authors.forename: Supathip
authors.initials: S
authors.lastname: Tujinda
authors.forename: Cheepsumon
authors.initials: C
authors.lastname: Suthippintawong
authors.forename: Munechika
authors.initials: M
authors.lastname: Enjoji
authors.forename: Manabu
authors.initials: M
authors.lastname: Nakashima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Vijittra
authors.initials: V
authors.lastname: Leardkamolkarn
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D045744:Cell Line, Tumor; D005770:Gastrointestinal Neoplasms / Q000235:genetics / Q000276:immunology* / Q000556:secondary; D006801:Humans; D017403:In Situ Hybridization; D008207:Lymphatic Metastasis; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000951:Antigens, Neoplasm; C120762:EBAG9 protein, human; D012333:RNA, Messenger; D012334:RNA, Neoplasm