title: Application of constant-current coulometry for estimation of plasma total antioxidant capacity and its relationship with transition metal contents.
abstract: Simple and express coulometric method for the evaluation of the total antioxidant capacity (TAC) of human plasma based on the reaction with electrogenerated bromine is applied. TAC of plasma from patients with different ethiology of chronic renal failure was observed. The levels of antioxidant capacity for venous and arterial plasma are authentically different (15+/-1 kCl/L versus 11.7+/-0.7 kCl/L, p<0.01). The application of Vitamin E and ximedon as an antioxidant treatment significantly increase TAC level of plasma. Free liposoluble antioxidants in plasma in alpha-tocopherol units was determined. Redox potential of plasma is measured and its correlation with lg(TAC) is obtained. Transition metal contents of Fe, Cu, Mn, Ni, and Cr in plasma of patients with chronic renal failure is significantly higher than that for a control group. Correlation analysis has shown negative linear regression between TAC value and transition metals concentration in plasma. This confirms interrelation of processes with participation of free radicals, antioxidants and transition metals as donors of electrons in chain radical processes. Moreover, it shows utility of common parameters, TAC for example, for estimation of efficiency of antioxidant defense system in living organism, in particular its antioxidant status.
authors.affiliation: Department of Analytical Chemistry, A.M. Butlerov Chemical Institute, Kazan State University, Kremlyevskaya 18, Kazan 420008, Russia. Ziyatdinovag@mail.ru
authors.forename: Guzel K
authors.initials: GK
authors.lastname: Ziyatdinova
authors.forename: Alexandr V
authors.initials: AV
authors.lastname: Voloshin
authors.forename: Albert Kh
authors.initials: AKh
authors.lastname: Gilmutdinov
authors.forename: Herman C
authors.initials: HC
authors.lastname: Budnikov
authors.forename: Talgat S
authors.initials: TS
authors.lastname: Ganeev
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis* / Q000627:therapeutic use; D003300:Copper / Q000097:blood; D004563:Electrochemistry / Q000379:methods; D005260:Female; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000097:blood; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000188:drug therapy; D008297:Male; D008345:Manganese / Q000097:blood; D008875:Middle Aged; D011743:Pyrimidines / Q000627:therapeutic use; D028561:Transition Elements / Q000097:blood*; D014810:Vitamin E / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D011743:Pyrimidines; D028561:Transition Elements; D014810:Vitamin E; C069705:xymedon; D008345:Manganese; D003300:Copper; D007501:Iron