Studies on the attachment of DNA to silica-coated nanoparticles through a Diels-Alder reaction.
A new method has been investigated for the functionalization of gold nanoparticles with DNA. Silica-coated nanoparticles functionalized with a maleimide have been prepared. These particles are designed to react with modified DNA containing a diene functionality at one end of the molecule. The result would be the formation of a more stable attachment of the DNA to the particle through a Diels-Alder reaction. This covalent attachment would not be susceptible to ligand exchanges, which are known to occur in the conventional DNA functionalization of gold nanoparticles.
Proupin-Perez
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Centre for Nanoscale Science, Department of Chemistry, The University of Liverpool Crown St, Liverpool L69 7ZD, UK. mproupin@liv.ac.uk
Cosstick
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R
Liz-Marzan
L M
LM
Salgueiriño-Maceira
V
V
Brust
M
M
D002244:Carbon / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D008024:Ligands; D046911:Macromolecular Substances; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D009841:Oligonucleotides / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
D008024:Ligands; D046911:Macromolecular Substances; D009841:Oligonucleotides; D002244:Carbon; D006046:Gold; D012822:Silicon Dioxide; D004247:DNA; D012825:Silicon