Regulation of human peptide transporter 1 (PEPT1) in gastric cancer cells by anticancer drugs.
Human peptide transporter 1 (PEPT1) mediates the cellular uptake of di- and tripeptides and peptide-like drugs in the small intestine. In the present study, we examined the regulation of PEPT1 by anticancer drugs in the gastric cancer cell line MKN45. PEPT1 was expressed and functioned in MKN45 cells. The transport activity and mRNA expression of the facilitative glucose transporter 1 (GLUT1) were significantly decreased by 5-fluorouracil treatment, but those of PEPT1 were slightly increased. Cisplatin treatment affected neither PEPT1 nor GLUT1 activity. In conclusion, PEPT1 expressed in MKN45 cells are resistant against the cellular injury induced by 5-fluorouracil and cisplatin.
Inoue
Mayumi
M
Department of Pharmacy, Faculty of Medicine, Kyoto University Hospital, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
Terada
Tomohiro
T
Okuda
Masahiro
M
Inui
Ken-Ichi
K
D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology*; D005472:Fluorouracil / Q000494:pharmacology*; D051272:Glucose Transporter Type 1 / Q000096:biosynthesis / Q000187:drug effects / Q000502:physiology; D006801:Humans; D000074011:Peptide Transporter 1; D012333:RNA, Messenger / Q000096:biosynthesis; D013274:Stomach Neoplasms / Q000473:pathology*; D027981:Symporters / Q000096:biosynthesis* / Q000187:drug effects / Q000502:physiology*; D014407:Tumor Cells, Cultured
D000964:Antimetabolites, Antineoplastic; D000970:Antineoplastic Agents; D051272:Glucose Transporter Type 1; D000074011:Peptide Transporter 1; D012333:RNA, Messenger; C082186:SLC15A1 protein, human; C495677:SLC2A1 protein, human; D027981:Symporters; D002945:Cisplatin; D005472:Fluorouracil