title: Expression of delayed rectifier potassium channels and their possible roles in proliferation of human gastric cancer cells.
abstract: Voltage-gated potassium (Kv) channels have been reported to be involved in the proliferation of many types of cells, including tumor cells. The overexpression of the Kv channels and related channel activity are involved in the neoplastic process. Our previous study has shown the existence of delayed rectifier potassium (I(K)) current in gastric cancer cells SGC7901. However, the expression and function of most delayed rectifier potassium (K(D)) channel subunits in gastric cancer cells are not completely resolved. Here we examine expression of K(D) channel subunits in Kv1-Kv3 families in immortalized gastric epithelial cells GES and various gastric cancer cells (including AGS, KATOIII, MKN28, MKN45, MGC803, SGC7901, SGC7901/ADR and SGC7901/VCR), and their roles in cell proliferation. RT-PCR analysis reveals that all cell lines examined express Kv1.3, Kv1.5, Kv1.6, Kv2.1 and Kv2.2. However, Kv1.2 and Kv3.2 genes are barely detectable in any given cancer cell lines. Kv1.5 protein, high mRNA levels in all cell lines examined, is also expressed in some cancer cells lines and more frequently detected in gastric cancer tissues. Downregulation of the expression of Kv1.5 in SGC7901 with RNA interference significantly inhibited the proliferation and tumorigenicity of SGC7901 cells. Moreover, in Ca(2+)-containing rather than Ca(2+)-free medium, KCl (50mM) stimulated a rapid increase in the concentration of cytosolic calcium in empty vector transfected cells that was blocked by verapamil. Likewise, decrease the expression of Kv1.5 with short interfering RNA also blocked the depolarization-induced influx of Ca(2+). This finding suggests that more than one kind of K(D) channel subunits are expressed in various gastric cancer cell lines. Kv1.5 may be involved in tumor cells proliferation by controlling Ca(2+) entry, and the interference of K(D) channels expression and/or activity could provide a novel strategy to reverse the malignant phenotype of gastric cancer cells.
authors.affiliation: State Key Laboratory of Cancer Biology & Institute of Digestive Disease, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Lan
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Shi
authors.forename: Zheyi
authors.initials: Z
authors.lastname: Han
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Hao
authors.forename: Yanglin
authors.initials: Y
authors.lastname: Pan
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Changcun
authors.initials: C
authors.lastname: Guo
authors.forename: Liu
authors.initials: L
authors.lastname: Hong
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Taidong
authors.initials: T
authors.lastname: Qiao
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D002118:Calcium / Q000378:metabolism; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051668:Delayed Rectifier Potassium Channels / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051666:Kv1.5 Potassium Channel / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D051668:Delayed Rectifier Potassium Channels; D051666:Kv1.5 Potassium Channel; D012333:RNA, Messenger; D002118:Calcium