An assessment of the effects of two types of bioresorbable barriers to prevent postoperative intra-abdominal adhesions in rats.
OBJECTIVE
This study assessed and compared the efficacy of two types of bioresorbable membranes in the prevention of postoperative adhesion under clean contaminated and bacterial peritonitis conditions using a cecal ligation and puncture model in rats.


METHODS
Wistar albino rats (n = 72) were divided into six groups. Bacterial peritonitis was induced using a cecal ligation and puncture model in groups 2, 4, and 6. Groups 1, 3, and 5 served as controls for clean contaminated procedures in the absence of bacterial peritonitis. Groups 1 and 2 were the untreated clean contaminated and bacterial peritonitis groups and served as controls for the effect of the bioresorbable membranes in each condition. In groups 3 and 4, a 1.5 x 3 cm USP glycerol/sodium hyaluronate/carboxymethylcellulose membrane was wrapped around the cecal resection area and a 2 x 4 cm membrane was left under the incision. The oxidized regenerated cellulose membrane was similarly applied in groups 5 and 6. Four weeks later, the adhesions were evaluated. In addition, fibrosis and inflammation were observed histopathologically.


RESULTS
Adhesion development (P = .008), fibrosis (P = .008), and inflammation (P = .0001) differed among the groups. Both materials increased adhesion formation in the bacterial peritonitis condition. Increased fibrotic activity was detected in all material-applied groups under both conditions. In addition, more inflammation was detected in the groups that received the application of a material, especially in the presence of bacterial peritonitis.


CONCLUSIONS
Neither material prevented adhesions in clean contaminated conditions. Moreover, they increased adhesion formation in bacterial peritonitis.
Kayaoglu
Huseyin Ayhan
HA
Department of General Surgery, Gaziosmanpasa University Medical School, Tokat, Turkey.
Ozkan
Namik
N
Hazinedaroglu
Selcuk Mevlut
SM
Ersoy
Omer Faik
OF
Koseoglu
Resit Dogan
RD
D000005:Abdomen; D000818:Animals; D001424:Bacterial Infections / Q000503:physiopathology / Q000601:surgery; D001672:Biocompatible Materials; D002480:Cellulase; D002483:Cellulose, Oxidized; D005355:Fibrosis; D005990:Glycerol; D006820:Hyaluronic Acid; D007249:Inflammation; D007813:Laparotomy / Q000009:adverse effects*; D008297:Male; D008567:Membranes, Artificial; D010538:Peritonitis / Q000503:physiopathology / Q000601:surgery; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000209:etiology / Q000473:pathology / Q000517:prevention & control
D001672:Biocompatible Materials; D002483:Cellulose, Oxidized; D008567:Membranes, Artificial; C118198:Seprafilm; D006820:Hyaluronic Acid; D002480:Cellulase; C019826:carboxymethylcellulase; D005990:Glycerol