title: Multiplex PCR for simultaneous detection of macrolide and tetracycline resistance determinants in streptococci.
abstract: Resistance to macrolides and tetracyclines is increasing among streptococci and co-occurs as their resistance determinants are carried on the same mobile element. We developed a multiplex PCR to facilitate simultaneous and specific detection of resistance determinants for both macrolides [erm(A), erm(B), and mef(A/E)] and tetracyclines [tet(M), tet(O), tet(K), and tet(L)] in streptococci.
authors.affiliation: Belgian Reference Center for Group A Streptococcus, University of Antwerp, Belgium. surbhi.malhotra@ua.ac.be
authors.forename: Surbhi
authors.initials: S
authors.lastname: Malhotra-Kumar
authors.forename: Christine
authors.initials: C
authors.lastname: Lammens
authors.forename: Jasper
authors.initials: J
authors.lastname: Piessens
authors.forename: Herman
authors.initials: H
authors.lastname: Goossens
mesh_terms: D024881:Drug Resistance, Bacterial / Q000235:genetics*; D018942:Macrolides / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013291:Streptococcus / Q000187:drug effects* / Q000235:genetics; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D018942:Macrolides