[Theoretical prediction of the antigenic epitopes of severe acute respiratory syndrome (SARS-CoV) proteins and evaluation of their diagnostic value].
Analysis of the sequence of a number of proteins of the virus causing severe acute respiratory syndrome (SARS-CoV) was used to theoretically predict antigenic epitopes. Nine sequences, encoding for the predicted epitopes in spike, nucleocapside, and membranous SARS CoV proteins, were synthesized by polymyrase chain reaction and cloned into the pGEX 4-T2 vector. The resultant plasmids were expressed in the E. coil cells and purified by glutathione-Sepharose affinity chromatography.
Ulanova
T I
TI
Puzyrev
V F
VF
Riabinina
S A
SA
Burkov
A N
AN
Obriadina
A P
AP
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics*; D000086862:Coronavirus M Proteins; D000086462:Coronavirus Nucleocapsid Proteins; D017931:DNA Primers; D000939:Epitopes / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000276:immunology; D017421:Sequence Analysis; D045169:Severe Acute Respiratory Syndrome / Q000175:diagnosis; D064370:Spike Glycoprotein, Coronavirus; D014169:Transformation, Bacterial; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000096:biosynthesis / Q000235:genetics*
D000956:Antigens, Viral; D000086862:Coronavirus M Proteins; D000086462:Coronavirus Nucleocapsid Proteins; D017931:DNA Primers; D000939:Epitopes; C000706237:M protein, SARS-CoV; D008562:Membrane Glycoproteins; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins