[Hairy root induced by wild-type agrobacterium rhizogenes K599 in soybean, cucumber and garden balsam in vivo].
The plantlets of soybean, cucumber and garden balsam were inoculated by wild-type Agrobacterium rhizogenes K599, and hairy root was induced on inoculated sites in vivo. The frequencies of hairy root induction from wound cotyledons of soybean, cucumber and garden balsam were 100%, 65% and 91%, respectively. Moreover, hairy root was induced from healthy cucumber axillary bud with frequency of 10%. PCR analysis of hairy root DNA was conducted using the primers from rolC gene. The PCR results showed that all hairy root lines contained T-DNA. The established system should be ideal for studying soybean and cucumber nematode and garden balsam breeding of flower dwarf architecture.
Xiang
Tai-He
TH
School of Life Sciences, Hangzhou Normal College, Hangzhou 310036, China. xthcn@163.com
Wang
Li-Lin
LL
Pang
Ji-Liang
JL
Chen
Min
M
Xu
Chao
C
D029068:Balsaminaceae / Q000235:genetics* / Q000254:growth & development; D018553:Cucumis sativus / Q000235:genetics* / Q000254:growth & development; D004269:DNA, Bacterial / Q000235:genetics; D015870:Gene Expression; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics*; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic
D004269:DNA, Bacterial; C036483:T-DNA