title: The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for Hepatitis B surface antigen.
abstract: A cucumber green mosaic mottle virus (CGMMV) full-length clone was developed for the expression of Hepatitis B surface antigen (HBsAg). The expression of the surface displayed HBsAg by the chimeric virus was confirmed through a double antibody sandwich ELISA. Assessment of the coat protein composition of the chimeric virus particles by SDS-PAGE analysis showed that 50% of the coat proteins were fused to the HBsAg. Biological activity of the expressed HBsAg was assessed through the stimulation of in vitro antibody production by cultured peripheral blood mononuclear cells (PBMC). PBMC that were cultured in the presence of the chimeric virus showed up to an approximately three-fold increase in the level of anti HBsAg immunoglobulin thus suggesting the possible use of this new chimeric virus as an effective Hepatitis B vaccine.
authors.affiliation: Institute of Biological Science, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Aikseng
authors.initials: A
authors.lastname: Ooi
authors.forename: Sianghee
authors.initials: S
authors.lastname: Tan
authors.forename: Rosmawati
authors.initials: R
authors.lastname: Mohamed
authors.forename: Noorsaadah Abdul
authors.initials: NA
authors.lastname: Rahman
authors.forename: Rofina Yasmin
authors.initials: RY
authors.lastname: Othman
mesh_terms: D000917:Antibody Formation / Q000276:immunology; D002478:Cells, Cultured; D018553:Cucumis sativus / Q000235:genetics* / Q000821:virology; D017799:Cucumovirus; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
chemical_list: D006514:Hepatitis B Surface Antigens; D011994:Recombinant Proteins