Morphology and metabolism of hepatocytes microencapsulated with acrylic terpolymer-alginate using gelatin and poly(vinyl alcohol) as extracellular matrices.
Microcapsules with good mechanical stability were prepared using an appropriate mixture of alginate and acrylic terpolymer. It was found from the microscopic observation that the microcapsules had a porous structure with interconnected pores, with a size of 50-150 nm. The results of the permeability experiment of microcapsules using FITC-dextrans showed that the capsule had a molecular mass cut-off of 120 kDa. The hepatocytes encapsulated in both alginate and acrylic terpolymer with gelatin and PVA rapidly aggregated in the core. The aggregated cells showed high albumin synthesis and ammonia removal, suggesting good metabolic function.
Moon
J S
JS
Department of Polymer Science, Kyungpook National University, Taegu 702-701, South Korea.
Jeon
H M
HM
Meng
W
W
Akaike
T
T
Kang
I K
IK
D000179:Acrylates / Q000737:chemistry*; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D017609:Barium Compounds / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D002214:Capsules; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D002712:Chlorides / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D005780:Gelatin / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism*; D006603:Hexuronic Acids / Q000737:chemistry; D008297:Male; D008970:Molecular Weight; D011142:Polyvinyl Alcohol / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012996:Solutions; D014867:Water / Q000737:chemistry
D000179:Acrylates; D000464:Alginates; D017609:Barium Compounds; D001672:Biocompatible Materials; D002214:Capsules; D002712:Chlorides; D003911:Dextrans; D005456:Fluorescent Dyes; D006603:Hexuronic Acids; D012996:Solutions; D014867:Water; C024986:barium chloride; D020723:Glucuronic Acid; D005780:Gelatin; D011142:Polyvinyl Alcohol; D016650:Fluorescein-5-isothiocyanate; C036658:acrylic acid