Oxidative stress in atherosclerosis and diabetes.
We measured the content of lipid peroxides in plasma LDL from patients with chronic CHD not accompanied by hypercholesterolemia; CHD and hypercholesterolemia; type 2 diabetes mellitus and decompensation of carbohydrate metabolism; and CHD, circulatory insufficiency, and type 2 diabetes mellitus (without hypercholesterolemia). The content of lipid peroxides in LDL isolated from blood plasma by differential ultracentrifugation in a density gradient was estimated by a highly specific method with modifications (reagent Fe(2+) xylene orange and triphenylphosphine as a reducing agent for organic peroxides). The content of lipid peroxides in LDL from patients was much higher than in controls (patients without coronary heart disease and diabetes). Hypercholesterolemia and diabetes can be considered as factors promoting LDL oxidation in vivo. Our results suggest that stimulation of lipid peroxidation in low-density lipoproteins during hypercholesterolemia and diabetes is associated with strong autooxidation of cholesterol and glucose during oxidative and carbonyl (aldehyde) stress, respectively. These data illustrate a possible mechanism of the progression of atherosclerosis in patients with diabetes mellitus.
Lankin
V Z
VZ
Laboratory for Biochemistry of Free Radical Processes, A. L. Myasnikov Institute of Cardiology, Russian Research-and-Production Center of Cardiology, Russian Ministry of Health, Moscow.
Lisina
M O
MO
Arzamastseva
N E
NE
Konovalova
G G
GG
Nedosugova
L V
LV
Kaminnyi
A I
AI
Tikhaze
A K
AK
Ageev
F T
FT
Kukharchuk
V V
VV
Belenkov
Yu N
YN
D000328:Adult; D050197:Atherosclerosis / Q000150:complications / Q000378:metabolism*; D008078:Cholesterol, LDL / Q000097:blood / Q000378:metabolism; D003327:Coronary Disease / Q000150:complications / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism*; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000150:complications / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D014461:Ultracentrifugation
D008078:Cholesterol, LDL; D008054:Lipid Peroxides