Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei.
A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.
U'Ren
Jana M
JM
Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640, USA.
Van Ert
Matthew N
MN
Schupp
James M
JM
Easterday
W Ryan
WR
Simonson
Tatum S
TS
Okinaka
Richard T
RT
Pearson
Talima
T
Keim
Paul
P
D001483:Base Sequence; D042726:Burkholderia mallei / Q000145:classification* / Q000235:genetics; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics; D017931:DNA Primers; D005798:Genes, Bacterial / Q000235:genetics; D005843:Geography; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D013045:Species Specificity; D019914:Taq Polymerase
D017931:DNA Primers; D009711:Nucleotides; D019914:Taq Polymerase