Assessment of nematode resistance in wheat transgenic plants expressing potato proteinase inhibitor (PIN2) gene.
Serine proteinase inhibitors (IP's) are proteins found naturally in a wide range of plants with a significant role in the natural defense system of plants against herbivores. The question addressed in the present study involves assessing the ability of the serine proteinase inhibitor in combating nematode infestation. The present study involves engineering a plant serine proteinase inhibitor (pin2) gene into T. durum PDW215 by Agrobacterium-mediated transformation to combat cereal cyst nematode (Heterodera avenae) infestation. Putative T(0) transformants were screened and positive segregating lines analysed further for the study of the stable integration, expression and segregation of the genes. PCR, Southern analysis along with bar gene expression studies corroborate the stable integration pattern of the respective genes. The transformation efficiency is 3%, while the frequency of escapes was 35.71%. chi(2) analysis reveals the stable integration and segregation of the genes in both the T(1) and T(2) progeny lines. The PIN2 systemic expression confers satisfactory nematode resistance. The correlation analysis suggests that at p < 0.05 level of significance the relative proteinase inhibitor (PI) values show a direct positive correlation vis-à-vis plant height, plant seed weight and also the seed number.
Vishnudasan
Dalia
D
Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi, 110 021, India.
Tripathi
M N
MN
Rao
Uma
U
Khurana
Paramjit
P
D000818:Animals; D001483:Base Sequence; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D011480:Protease Inhibitors / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics*; D014170:Transformation, Genetic; D014908:Triticum / Q000235:genetics* / Q000469:parasitology*; D017187:Tylenchida / Q000472:pathogenicity*
D004274:DNA, Recombinant; D010940:Plant Proteins; D011480:Protease Inhibitors