The use of phase inversion temperature (PIT) microemulsion technology to enhance oil utilisation during Streptomyces rimosus fed-batch fermentations to produce oxytetracycline.
The use of a rapeseed oil emulsion feed, produced by a phase inversion temperature (PIT) process, produced more biomass, gave a 3-fold increase in oil utilisation and a higher oxytetracycline titre but a higher residual oil concentration when compared to a conventional fed-batch Streptomyces rimosus process fed with crude rapeseed oil. Importantly, microbial utilisation of the surfactant was confirmed for the first time.
Papapanagiotou
Panos A
PA
Biochemical Engineering, Department of Chemical Engineering, The University of Birmingham, B15 2TT, Edgbaston, Birmingham, UK.
Quinn
Henry
H
Molitor
Jean-Pierre
JP
Nienow
Alvin W
AW
Hewitt
Christopher J
CJ
D019149:Bioreactors; D003470:Culture Media; D004655:Emulsions; D007218:Industrial Microbiology / Q000379:methods; D009821:Oils / Q000378:metabolism*; D010118:Oxytetracycline / Q000096:biosynthesis*; D013302:Streptomyces / Q000254:growth & development*; D013696:Temperature
D003470:Culture Media; D004655:Emulsions; D009821:Oils; D010118:Oxytetracycline