title: [Effects of Angelica sinensis polysaccharide on cell-mediated immunity].
abstract: OBJECTIVE
  To investigate the effects of Angelica sinensis polysaccharide (AP) on cell-mediated immunity.
  
  
  METHODS
  The lymphocyte proliferation was tested by MTT colormetry; T lymphocyte fraction were separated with immune magnetic beads and the change of CD4(+) T lymphocyte percentage was detected by flow cytometry.
  
  
  RESULTS
  In the range of 30-300 mg/L, the total AP and its three fractions, ie AP-0, AP-1, AP-2, AP-3, directly stimulated the proliferation of murine splenocytes, and AP-3 significantly enhanced the proliferation of lymphocytes in MLR and T lymphocytes. The percentage of CD4(+) T lymphocytes in total splenocytes was also enhanced by AP-3.
  
  
  CONCLUSIONS
  AP can promote the cell-mediated immunity with T lymphocytes as one of its target cells.
authors.affiliation: Department of Pharmacology, Fourth Military Medical University, Xi'an 710032, China. yangtiehong@sina.com
authors.forename: Tie-hong
authors.initials: TH
authors.lastname: Yang
authors.forename: Min
authors.initials: M
authors.lastname: Jia
authors.forename: Qi-bing
authors.initials: QB
authors.lastname: Mei
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry; D007111:Immunity, Cellular / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D011134:Polysaccharides