Direct determination of glucuronide and sulfate of p-hydroxymethamphetamine in methamphetamine users' urine.
Two conjugates of p-hydroxymethamphetamine (p-OHMA), p-OHMA-glucuronide (p-OHMA-Glu) and p-OHMA-sulfate (p-OHMA-Sul) have been identified in methamphetamine (MA) users' urine by using liquid chromatography-high resolution tandem mass spectrometry (LC-HRMS-MS). The synthesis of p-OHMA-Glu and p-OHMA-Sul, and an LC-MS procedure for the simultaneous determination of MA and its four metabolites, amphetamine (AP), p-OHMA, p-OHMA-Glu and p-OHMA-Sul, in urine have also been established. After deproteinizing urine samples with methanol, LC-MS employing a C(18) semi-micro column with a gradient elution program provided the successful separations and MS determinations of these analytes within 20 min. Based on the established method, p-OHMA-Sul was detected at higher concentrations than p-OHMA-Glu in all of the three urine samples tested. These data suggest that sulfation is a major pathway in the MA phase II metabolism.
Shima
Noriaki
N
Forensic Science Laboratory, Osaka Prefectural Police Headquarters, Chuo-ward, Japan. nori6499@amber.plala.or.jp
Tsutsumi
Hiroe
H
Kamata
Tooru
T
Nishikawa
Mayumi
M
Katagi
Munehiro
M
Miki
Akihiro
A
Tsuchihashi
Hitoshi
H
D019969:Amphetamine-Related Disorders / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods; D020719:Glucuronides / Q000652:urine; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D008694:Methamphetamine / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000652:urine; D013431:Sulfates / Q000652:urine
D020719:Glucuronides; D013431:Sulfates; D008694:Methamphetamine; C005877:pholedrine