Patterning cultured cells by visible light illumination with photosensitizers.
We showed that PC12 cells and 3T3 cells cultured in dishes were killed by illumination with visible white light from a halogen lamp at 7 x 10(4) lx for 5 min in the presence of either 10 microM hematoporphyrin or 2 microM methylene blue as a photosensitizer. This simple technique, based on the photodynamic reaction via generation of reactive oxygen species can be applicable for patterning cultured cells.
Waki
Ryoji
R
Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Sakai, Osaka 599-8570, Japan.
Gamo
Sumiko
S
Bessho
Masahiko
M
Hara
Masayuki
M
D016475:3T3 Cells; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D006415:Hematoporphyrins / Q000494:pharmacology*; D008027:Light; D008751:Methylene Blue / Q000494:pharmacology*; D051379:Mice; D016716:PC12 Cells; D017319:Photosensitizing Agents / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
D006415:Hematoporphyrins; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D008751:Methylene Blue