title: Development and validation of a capillary high-performance liquid chromatography/electrospray tandem mass spectrometric method for the quantification of bisphenol A in air samples.
abstract: Bisphenol A (BPA) is a toxic industrial chemical that affects the endocrine system even at low concentrations. A new method, based on capillary high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) analysis, has been developed to determine BPA in atmospheric samples. The method involves collection of air samples (typically 2 m(3)) on glass fiber filters, with ultrasonic extraction and sample concentration under vacuum before analysis. HPLC analysis was performed isocratically at a flow rate of 10 microL min(-1) using a capillary reversed-phase column and MS/MS analysis in negative ion multiple reaction monitoring (MRM) mode, using BPA-d(16) as internal standard. The present method provides linear response in the range 0.007-3.5 microg/filter (R(2) > 0.999) and is characterized by high accuracy (mean bias 2%) and good reproducibility (mean RSD 5%). High sensitivity (LOD = 2 ng/m(3) based on 2 m(3) of air collected), specificity, and speed of the analysis make the present method suitable for routine determination of BPA in the atmosphere, both for ambient and personnel monitoring.
authors.affiliation: Occupational Medicine Unit, University of Bologna, S. Orsola-Malpighi Hospital, via Palagi 9, 40138 Bologna, Italy. l.sabatini@unibo.it
authors.forename: Laura
authors.initials: L
authors.lastname: Sabatini
authors.forename: Anna
authors.initials: A
authors.lastname: Barbieri
authors.forename: Francesco Saverio
authors.initials: FS
authors.lastname: Violante
mesh_terms: D000395:Air Pollutants, Occupational / Q000032:analysis* / Q000737:chemistry; D001559:Benzhydryl Compounds; D002617:Chemical Industry; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods*; D006727:Hormone Antagonists / Q000032:analysis / Q000737:chemistry; D006801:Humans; D016273:Occupational Exposure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D000395:Air Pollutants, Occupational; D001559:Benzhydryl Compounds; D006727:Hormone Antagonists; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds