Damaging effects of gliadin on three-dimensional cell culture model.
OBJECTIVE
To evaluate the effects of gliadin on the oxidative environment in the "in vivo-like" model of a three-dimensional cell culture system.


METHODS
LoVo cell line (intestinal adenocarcinoma) multicellular spheroids were treated with digested gliadin (with albumin used as a control). Spheroid volumes, cell viability and morphology, lactate dehydrogenase (LDH) release, content of reduced glutathione (GSH) and activity of GSH-related enzymes were examined. The data were statistically analyzed using the Student's t-test. was considered statistically significant.


RESULTS
Gliadin reduced cell viability (from 20% to 60%) and led to morphological alterations characterized by apoptotic findings and cytoskeletal injuries. LDH activity increased. The content of GSH reduced (-20% vs controls), and activity of GSH-related enzymes was significantly inhibited.


CONCLUSIONS
Gliadin treatment induces an imbalance in the antioxidative mechanism of cells cultured by the three-dimensional technique. This alteration may explain the cell damage directly caused by gliadin and the subsequent morphological abnormalities.
Dolfini
Ersilia
E
Department of Gastroenterology, Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena IRCCS, Via F. Sforza 35, Milan 20122, Italy.
Elli
Luca
L
Roncoroni
Leda
L
Costa
Barbara
B
Colleoni
Maria-Pia
MP
Lorusso
Vito
V
Ramponi
Simona
S
Braidotti
Paola
P
Ferrero
Stefano
S
Falini
Maria-Letizia
ML
Bardella
Maria-Teresa
MT
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005903:Gliadin / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007414:Intestinal Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D018874:Spheroids, Cellular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D005903:Gliadin; D007770:L-Lactate Dehydrogenase; D005978:Glutathione