Genetic structure of wild rice Oryza glumaepatula populations in three Brazilian biomes using microsatellite markers.
The existence of Oryza glumaepatula is threatened by devastation and, thus, the implementation of conservation strategies is extremely relevant. This study aimed to characterize the genetic variability and estimate population parameters of 30 O. glumaepatula populations from three Brazilian biomes using 10 microsatellite markers. The levels of allelic variability for the SSR loci presented a mean of 10.3 alleles per locus and a value of 0.10 for the average allelic frequency value. The expected total heterozygosity (H(e)) ranged from 0.63 to 0.86. For the 30 populations tested, the mean observed (H(o)) and expected heterozygosities (H(e)) were 0.03 and 0.11 within population, respectively, indicating an excess of homozygotes resulting from the preferentially self-pollinating reproduction habit. The estimated fixation index ( (IS) ) was 0.79 that differed significantly from zero, indicating high inbreeding within each O. glumaepatula population. The total inbreeding of the species ((IT) ) was 0.98 and the genetic diversity indexes among populations, (ST) and (ST), were 0.85 and 0.90, respectively, indicating high genetic variability among them. Thus, especially for populations located in regions threatened with devastation, it is urgent that in situ preservation conditions should be created or that collections be made for ex situ preservation to prevent loss of the species genetic variability.
Vianello Brondani
Rosana Pereira
RP
Laboratório de Biotecnologia Vegetal, Embrapa-CNPAF, C.P. 179, 74001-970 GO, Goiânia, Brazil. rosanavb@cnpaf.embrapa.br
Zucchi
Maria Imaculada
MI
Brondani
Claudio
C
Nakano Rangel
Paulo Hideo
PH
De Oliveira Borba
Tereza Cristina
TC
Rangel
Priscila Nascimento
PN
Magalhães
Mara Rubia
MR
Vencovsky
Roland
R
D000483:Alleles; D001938:Brazil; D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D018745:Genome, Plant; D006579:Heterozygote; D006720:Homozygote; D018895:Microsatellite Repeats; D012275:Oryza / Q000235:genetics*; D012098:Reproduction / Q000235:genetics
D018744:DNA, Plant