title: Perylene attached to DNA through stiff or flexible linker: duplex stability and FRET.
abstract: Two fluorescent nucleosides, 5-(perylen-3-ylethynyl)-2'-deoxyuridine and 5-[(perylen-3-yl)methoxypropyn-1-yl]-2'-deoxyuridine, were incorporated into synthetic oligodeoxyribonucleotides and spectral properties of the conjugates and their duplexes were studied.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Skorobogatyj
authors.forename: Anna A
authors.initials: AA
authors.lastname: Pchelintseva
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Ustinov
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Korshun
authors.forename: Andrei D
authors.initials: AD
authors.lastname: Malakhov
mesh_terms: D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes; D009705:Nucleosides / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D010569:Perylene / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D009692:Nucleic Acid Heteroduplexes; D009705:Nucleosides; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D010569:Perylene; D004247:DNA