title: Intratumour T cells, their activation status and survival in gastric carcinomas characterised for microsatellite instability and Epstein-Barr virus infection.
abstract: Gastric carcinomas (GCs) with high microsatellite instability (MSI) or an Epstein-Barr virus (EBV) infection are prevalently poorly differentiated adenocarcinomas with abundant lymphoid infiltration. The aims of the study were to clarify (1) if tumour-infiltrating lymphocytes (TILs) and cytotoxic-activated TILs are associated with a better clinical outcome in patients with GCs characterised for the presence of MSI and EBV; (2) if the nature and the activation status of TILs are involved in tumour cell apoptosis, evaluated using the M30 antibody, directed against a fragment of cytokeratin-18 caspase-cleaved during early steps of epithelial cell apoptosis. The immunophenotype of TILs and the tumour cell apoptosis were analysed with immunohistochemistry in 96 GCs, including 35 MSI GCs, and 61 GCs without MSI [microsatellite stable (MSS)], 17 of which were EBV+. MSI and MSS/EBV+ GCs displayed a significantly higher mean number of cytotoxic-activated TILs and apoptotic tumour cells than MSS/EBV- GCs (CD8+ TILs/HPF, 21.7 and 69.6 vs 6.4; T-cell intracellular antigen (TIA)-1+ TILs/HPF, 16.7 and 32.05 vs 5.2; granzyme B+ TILs/HPF, 7.5 and 8.6 vs 0.8; perforin+ TILs/HPF, 5.9 and 9.2 vs 0.9; and M30 IR tumour cells, 5.9 and 2.9 vs 2.3%). In addition to the most reliable clinico-pathological parameters (lymph node status, depth of tumour invasion and tumour stage), a univariate analysis showed that the presence of CD3+ TILs higher than 14.9 (p=0.01), CD8+ TILs higher than 9.5 (p<0.05) and MSI (p=0.02) were associated with better overall patient survival. Using a Cox regression model, only a high number of CD3+ TILs (p=0.02) and a low tumour stage (p=0.00001) were identified as independent prognostic factors. In conclusion, our study demonstrates that a high number of CD3+ and CD8+ TILs is a characteristic of MSI- and EBV-associated GCs and represents a favourable prognostic factor, independently of the pathogenesis of GCs.
authors.affiliation: Department of Human Morphology, Section of Anatomic Pathology, University of Insubria and Ospedale di Circolo, Varese, Italy. annamaria.chiaravalli@tin.it
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Chiaravalli
authors.forename: Maddalena
authors.initials: M
authors.lastname: Feltri
authors.forename: Valentina
authors.initials: V
authors.lastname: Bertolini
authors.forename: Elena
authors.initials: E
authors.lastname: Bagnoli
authors.forename: Daniela
authors.initials: D
authors.lastname: Furlan
authors.forename: Roberta
authors.initials: R
authors.lastname: Cerutti
authors.forename: Raffaele
authors.initials: R
authors.lastname: Novario
authors.forename: Carlo
authors.initials: C
authors.lastname: Capella
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000401:mortality* / Q000821:virology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D017252:CD3 Complex / Q000378:metabolism; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000473:pathology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation / Q000276:immunology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008297:Male; D053842:Microsatellite Instability; D008875:Middle Aged; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000276:immunology / Q000401:mortality* / Q000821:virology; D015996:Survival Rate; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D017252:CD3 Complex