title: Arsenate respiratory reductase gene (arrA) for Desulfosporosinus sp. strain Y5.
abstract: Desulfosporosinus sp. strain Y5 is a spore-forming bacterium capable of dissimilatory arsenate reduction coupled to the oxidation of aromatic compounds. In arsenate respiration, the arsenate respiratory reductase (ARR) catalyzes the reduction of arsenate to arsenite. Our objective is to characterize the arrA gene, encoding the ARR, for Desulfosporosinus sp. strain Y5. Oligonucleotide primers were designed based on the few arrA gene sequences available at the time and validated against positive and negative controls. The resulting arrA-amplicon of approximately 2.0kb was cloned and sequenced. The arrA from Desulfosporosinus sp. Y5 is closely related to Desulfitobacterium hafniense (similarity of 77% and 81% at the nucleotide and amino acid levels, respectively). Phylogenetic topology based on the arrA gene was partially congruent with that of 16S rRNA-based analysis. This arrA sequence will support the development of specific tracking probes for Desulfosporosinus sp. Y5 and the molecular characterization and monitoring of dissimilatory arsenate reducing bacteria.
authors.affiliation: Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
authors.forename: José R
authors.initials: JR
authors.lastname: Pérez-Jiménez
authors.forename: Christopher
authors.initials: C
authors.lastname: DeFraia
authors.forename: L Y
authors.initials: LY
authors.lastname: Young
mesh_terms: D000595:Amino Acid Sequence; D053501:Arsenite Transporting ATPases; D001483:Base Sequence; D003013:Clostridium / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D016623:Ion Pumps / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D016623:Ion Pumps; D009097:Multienzyme Complexes; D053501:Arsenite Transporting ATPases