Comparative genomic hybridization analysis of cutaneous large B-cell lymphomas.
The aim of the present study was to identify genetic aberrations in a series of patients with cutaneous large B-cell lymphoma (LBCL) using comparative genomic hybridization (CGH). Eighteen consecutive patients with primary (13 patients) (PCLBCL) and secondary (five patients) (SCLBCL) cutaneous large B-cell lymphoma were included in the study. Nine cases corresponded to PCLBCL leg type and four cases primary cutaneous follicle centre-cell lymphoma (PCFCL). Chromosomal imbalances (CIs) were detected in 14 of 18 samples (77.8%). All of nine cases with PCLBCL leg type and two of four cases with PCFCL showed CIs (100% and 50%, respectively). Regarding SCLBCL, in three of five cases (60%), CIs were detected. The most frequently detected gains involved 2q, 5q, 3 and 7q and amplifications affected 18, 12 and 13. Frequent losses were found in 17p. In PCLBCL leg type, the most frequent gains involved 2q and 7q, amplifications were localized in chromosomes 12, 13 and 18 and losses affected chromosomes 17p and 19. In PCFCL, gains located in 3q, 4 and 7q were found. Our study seems to confirm clear-cut differences between primary cutaneous LBCL and nodal diffuse LBCL, and it suggests the presence of genotypic differences between cases of PCLBCL leg type and cases of PCFCL.
Giménez
Sònia
S
Laboratori de Citogenètica i Biologia Molecular, Servei de Patologia, Hospital del Mar (IMAS), Unitat de Recerca en Neoplàsies Hematològiques, PRBB, Barcelona, Spain.
Costa
Carlota
C
Espinet
Blanca
B
Solé
Francesc
F
Pujol
Ramon M
RM
Puigdecanet
Eulàlia
E
García-Moreno
Pilar
P
Sánchez
Júlia
J
Gallardo
Fernando
F
Estrach
Teresa
T
García-Muret
Pilar
P
Romagosa
Vicenç
V
Serrano
Sergi
S
Servitje
Octavio
O
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D012878:Skin Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology