title: Sensitivity to radiation-induced apoptosis and neuron loss declines rapidly in the postnatal mouse neocortex.
abstract: Therapeutic brain irradiation can cause progressive decline in cognitive function, particularly in children, but the reason for this effect is unclear. The study explored whether age-related differences in apoptotic sensitivity might contribute to the increased vulnerability of the young brain to radiation. Postnatal day 1 (P1) to P30 mice were treated with 0-16 Gy whole-body X-irradiation. Apoptotic cells were identified and quantified up to 48 h later using the TdT-UTP nick end-labelling method (TUNEL) and immunohistochemistry for activated caspase-3. The number of neuron-specific nuclear protein (NeuN)-positive and -negative cells were also counted to measure neuronal and non-neuronal cell loss. Significantly greater TUNEL labelling occurred in the cortex of irradiated P1 animals relative to the other age groups, but there was no difference among the P7, P14 and P30 groups. Irradiation decreased the %NeuN-positive cells in the mice irradiated on P1, whereas in P14 animals, irradiation led to an increase in the %NeuN-positive cells. These data demonstrate that neocortical neurons of very young mice are more susceptible to radiation-induced apoptosis. However, this sensitivity decreases rapidly after birth. By P14, acute cell loss due to radiation occurs primarily in non-neuronal populations.
authors.affiliation: Department of Neurological Surgery, University of Pittsburgh, PA 15213, USA.
authors.forename: K
authors.initials: K
authors.lastname: Nakaya
authors.forename: T
authors.initials: T
authors.lastname: Hasegawa
authors.forename: J C
authors.initials: JC
authors.lastname: Flickinger
authors.forename: D S
authors.initials: DS
authors.lastname: Kondziolka
authors.forename: W
authors.initials: W
authors.lastname: Fellows-Mayle
authors.forename: G T
authors.initials: GT
authors.lastname: Gobbel
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D017209:Apoptosis / Q000528:radiation effects*; D002648:Child; D002657:Child Development; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019579:Neocortex / Q000166:cytology / Q000254:growth & development / Q000528:radiation effects*; D009474:Neurons / Q000528:radiation effects*; D011832:Radiation Injuries / Q000503:physiopathology*; D012307:Risk Factors