[Enhancement of wound healing by taspine and its effect on fibroblast].
OBJECTIVE
To study the effect of taspine on enhancement of skin wound healing and its effect on fibroblast proliferation and autocrine.


METHODS
The plerosis effect of taspine on experimental skin wound was observed in vivo. Different concentrations of taspine were added in vitro and MTT technique was applied to observe its effect on fibroblast proliferation, the levels of transforming growth factor-beta1 (TGF-13P) and epidermal growth factor (EGF) were determined by ELISA.


RESULTS
In vivo, exo-applied taspine 300 microg and 150 microg accelerated the recovery of skin wound. In vitro, 0.50-0.4 microg/ml taspine could increase autocrine of TGF-beta1and EGF by fibroblast, but it showed no effect on L929 fibroblast proliferation.


CONCLUSIONS
Taspine enhances wound healing by increasing the autocrine of TGF-beta1 and EGF by fibroblast.
Dong
Yalin
Y
School of medicine, Xi' an Jiaotong University, Xi' an 71006.
He
Langchong
L
Chen
Fang
F
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D028145:Caulophyllum / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D004815:Epidermal Growth Factor; C018394:taspine