Stimulated endothelial cell adhesion and angiogenesis with laminin-5 modification of expanded polytetrafluoroethylene.
Biomedical implants often exhibit poor clinical performance due to the formation of a periimplant avascular fibrous capsule. Surface modification of synthetic materials has been evaluated to accelerate the formation of functional microcirculation in association with implants. The current study used a flow-mediated protein deposition system to modify expanded polytetrafluoroethylene (ePTFE) with a laminin-5-rich conditioned growth medium and with medium from which laminin-5 had been selectively removed. An in vitro model of endothelial cell adherence determined that laminin-5 modification resulted in significantly increased adhesion of human microvessel endothelial cells to ePTFE. In vivo studies evaluating the periimplant vascular response to laminin-5-treated samples indicated that absorption of laminin-5-rich conditioned medium supported accelerated neovascularization of ePTFE implants. A flow system designed to treat porous implant materials facilitates laminin-5 modification of commercially available ePTFE, resulting in increased endothelial cell adhesion in vitro and increased vascularization in vivo.
Kidd
Kameha R
KR
Biomedical Engineering Program University of Arizona, Tucson, Arizona 85724, USA.
Dal Ponte
Donny
D
Stone
Alice L
AL
Hoying
James B
JB
Nagle
Raymond B
RB
Williams
Stuart K
SK
D000273:Adipose Tissue / Q000276:immunology / Q000502:physiology; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000648:ultrastructure; D002460:Cell Line; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000493:pharmacokinetics; D004730:Endothelium, Vascular / Q000166:cytology* / Q000648:ultrastructure; D006652:Histological Techniques; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008833:Microcirculation; D018919:Neovascularization, Physiologic; D011138:Polytetrafluoroethylene; D019736:Prostheses and Implants; D014471:Umbilical Veins / Q000166:cytology; D000097794:Kalinin
D001672:Biocompatible Materials; D015815:Cell Adhesion Molecules; D017077:Culture Media, Conditioned; D011138:Polytetrafluoroethylene; D000097794:Kalinin