[Induction of nicotinic acid hydroxylase activity of Pseudomonas putida NA-1 and optimization of transformation conditions].
Some features of Pseudomonas putida NA-1 strain in cultivation and enzyme production were different from S. marcescens IFO 12648 and P. fluorescens TN5 stains which could transform nicotinic acid to 6-hydroxynicotinic acid reported by other scientists, such as optimal carbon and its optimal concentration, optimal inducer concentration, and optimal cultivation temperature. The ideal transformation condition was nicotinic acid 3%, temperature 35 degrees C and pH 7.0. Under an appropriate condition, in a 4 liter fermenter, production yield of 6-hydroxynicotinic acid by Pseudomonas putida NA-1 was 108.39 g/L.
Lu
Wei-Hong
WH
Jiangsu Provincial Key Laboratory for Bioresource Technology, College of Life Science, Nanjing Normal University, Nanjing 210097, China. luweihong168@sohu.com
Wang
Xin
X
Xu
Li
L
Dai
Yi-Jun
YJ
Yuan
Sheng
S
D001711:Biotransformation; D004790:Enzyme Induction; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006900:Hydroxylation; D009525:Niacin / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000096:biosynthesis*; D016958:Pseudomonas putida / Q000201:enzymology*; D013696:Temperature
D009525:Niacin; C021862:nicotinate dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors