Colorimetric bio-barcode amplification assay for cytokines.
The bio-barcode amplification assay has become a powerful tool in detecting tens to hundreds of biological targets such as proteins and nucleic acids in the entire sample. However, current bio-barcode detection schemes still require many experimental steps including microarrayer-based immobilization of oligonucleotides on a glass chip, silver enhancement of immobilized gold nanoparticles on a chip, and light-scattering measurement. Here, we report a colorimetric bio-barcode method that minimizes the above requirements while detecting 30 aM concentrations of cytokines (approximately 3 orders of magnitude more sensitive than conventional nonenzymatic cytokine detection assays). The assay is based on porous microparticles, which enable loading of a large number of barcode DNA per particle, and gold nanoparticle-based colorimetric barcode detection method.
Nam
Jwa-Min
JM
Department of Chemistry, University of California, Berkeley, California 94720, USA.
Wise
Amber R
AR
Groves
Jay T
JT
D003124:Colorimetry / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry; D001330:Electronic Data Processing / Q000379:methods*; D006046:Gold; D007376:Interleukin-2 / Q000032:analysis* / Q000235:genetics; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry; D015335:Molecular Probes / Q000737:chemistry / Q000648:ultrastructure; D016062:Porosity
D015342:DNA Probes; D007376:Interleukin-2; D015335:Molecular Probes; D006046:Gold