Affinity of (alpha-P-borano)-NTP analogs to rabbit muscle pyruvate kinase.
The binding affinity of (alpha-P-borano) and other NTP analogs to rabbit muscle pyruvate kinase (PK) was investigated using a fluorescence quenching approach to obtain structure-activity relationships for substrate specificity of nucleotide analogs.
Dobrikov
Mikhail I
MI
Department of Chemistry, P.M. Gross Chemical Laboratory, Duke University, Durham, NC 27705, USA.
Shaw
Barbara Ramsay
BR
D000244:Adenosine Diphosphate / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D001880:Boranes / Q000737:chemistry; D018360:Crystallography, X-Ray; D015226:Dinucleoside Phosphates / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006160:Guanosine Triphosphate / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods; D009132:Muscles / Q000201:enzymology*; D009711:Nucleotides / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D011770:Pyruvate Kinase / Q000737:chemistry*; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013816:Thermodynamics
D001880:Boranes; D015226:Dinucleoside Phosphates; D009711:Nucleotides; D010710:Phosphates; D000244:Adenosine Diphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D011770:Pyruvate Kinase