title: Effects of routine prophylactic vaccination or administration of aluminum adjuvant alone on allergen-specific serum IgE and IgG responses in allergic dogs.
abstract: OBJECTIVE
  To determine the acute corn-specific serum IgE and IgG, total serum IgE, and clinical responses to s.c. administration of prophylactic vaccines and aluminum adjuvant in corn-allergic dogs.
  
  
  METHODS
  20 allergic and 8 nonallergic dogs.
  
  
  METHODS
  17 corn-allergic dogs were vaccinated. Eight clinically normal dogs also were vaccinated as a control group. Serum corn-specific IgE, corn-specific IgG, and total IgE concentrations were measured in each dog before vaccination and 1 and 3 weeks after vaccination by use of an ELISA. The corn-allergic dogs also had serum immunoglobulin concentrations measured at 8 and 9 weeks after vaccination. Twenty allergic dogs received a s.c. injection of aluminum adjuvant, and serum immunoglobulin concentrations were measured in each dog 1, 2, 3, 4, and 8 weeks after injection. The allergic dogs were examined during the 8 weeks after aluminum administration for clinical signs of allergic disease.
  
  
  RESULTS
  The allergic dogs had significant increases in serum corn-specific IgE and IgG concentrations 1 and 3 weeks after vaccination but not 8 or 9 weeks after vaccination. Control dogs did not have a significant change in serum immunoglobulin concentrations after vaccination. After injection of aluminum adjuvant, the allergic dogs did not have a significant change in serum immunoglobulin concentrations or clinical signs.
  
  
  CONCLUSIONS
  Allergen-specific IgE and IgG concentrations increase after prophylactic vaccination in allergic dogs but not in clinically normal dogs. Prophylactic vaccination of dogs with food allergies may affect results of serologic allergen-specific immunoglobulin testing performed within 8 weeks after vaccination.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
authors.forename: Kathy C
authors.initials: KC
authors.lastname: Tater
authors.forename: Hilary A
authors.initials: HA
authors.lastname: Jackson
authors.forename: Judy
authors.initials: J
authors.lastname: Paps
authors.forename: Bruce
authors.initials: B
authors.lastname: Hammerberg
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000535:Aluminum; D000704:Analysis of Variance; D000818:Animals; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005512:Food Hypersensitivity / Q000276:immunology / Q000662:veterinary*; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary*; D003313:Zea mays / Q000009:adverse effects*
chemical_list: D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D000535:Aluminum