title: Distribution, localization, and phylogeny of abundant populations of Crenarchaeota in anaerobic granular sludge.
abstract: Eight anaerobic granular sludges were surveyed for Crenarchaeota using rRNA gene cloning. Microbial arrangement and substrate uptake patterns were elucidated by fluorescent in situ hybridization and beta imaging. Group 1.3 Crenarchaeota represented up to 50% of Archaea and 25% of the total microbiota in five sludges. Crenarchaeota were localized in close association with methanogenic Archaea.
authors.affiliation: Microbial Ecology Laboratory, Department of Microbiology and Environmental Change Institute, National University of Ireland, Galway, University Road, Galway, Ireland.
authors.forename: Gavin
authors.initials: G
authors.lastname: Collins
authors.forename: Leanne
authors.initials: L
authors.lastname: O'Connor
authors.forename: Thérèse
authors.initials: T
authors.lastname: Mahony
authors.forename: Armin
authors.initials: A
authors.lastname: Gieseke
authors.forename: Dirk
authors.initials: D
authors.lastname: de Beer
authors.forename: Vincent
authors.initials: V
authors.lastname: O'Flaherty
mesh_terms: D000693:Anaerobiosis; D019615:Crenarchaeota / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D019641:DNA, Archaeal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage