Effect of bowel decontamination with metronidazole and vancomycin on gastroduodenal myoelectric activity.
It is well recognized that prolonged antibiotic therapy leading to gut decontamination often results in side effects and may lead to colonization of gut with pathologic bacteria. Changes of a gut microflora could play a role in dysmotility of gastrointestinal tract. The aim of the study was to evaluate influence of intraluminal colon anaerobic and aerobic bacterial flora on myoelectric activity of duodenum and stomach. A myoelectric activity recordings using electrodes implanted on small bowel of the conscious rats were performed. Group I was scheduled for control recording, group II for recordings in 4th day after metronidazole (M) administration (30 mg/kg) and group III for recordings after vancomycin (V) administration (15 mg/kg) respectively. Rat's stools were cultured for confirmation of changes in colon flora composition. Recordings were previously filtered digitally with bandwidth filter 0.01-0.1 Hz and 0.1-1.0 Hz to extract gastric and duodenal slow wave respectively and than analyzed with Fast Fourier Transformation. Baseline duodenal slow wave frequency in control group revealed 0.60 +/- 0.05 Hz. M increased slow waves frequency to 0.64 +/- 0.13 Hz and V did not 0.58 +/- 0.09 Hz (p > 0.05). Slow wave dominant frequency of the stomach showed decrease of frequency from control 0.035 +/- 0.04 to 0.025 +/- 0.06 Hz after M (p < 0.05). Pretreatment with V also did not influence slow wave dominant frequency in comparison to control group (0.036 +/- 0.07 Hz, p > 0.05).


CONCLUSIONS
Only pretreatment with M significantly decreased gastric slow wave frequency. One can speculate that M effects are related not only to gut decontamination but also directly affects ENS. We propose hypothesis that M influence on slow wave frequency may be related not only to its antimicrobial activity but to its potential neurotoxic action on intramural ENS neurons.
Królczyk
Grzegorz
G
Katedra Patofizjologii, Collegium Medicum Uniwersytetu Jagiellońskiego.
Czupryna
Antoni
A
Sobocki
Jacek
J
Nowak
Lukasz
L
Zurowski
Daniel
D
Szatyłowiczi
Jadwiga
J
Strus
Magdalena
M
Thor
Piotr J
PJ
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D004386:Duodenum / Q000382:microbiology / Q000503:physiopathology; D005260:Female; D005769:Gastrointestinal Motility / Q000187:drug effects*; D008795:Metronidazole / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000382:microbiology / Q000503:physiopathology; D014640:Vancomycin / Q000494:pharmacology*
D000890:Anti-Infective Agents; D008795:Metronidazole; D014640:Vancomycin