Characterization by Mass Spectroscopy of a 10 kDa c-554 Cytochrome from the Green Sulfur Bacterium Chlorobium tepidum.
Preparative isoelectric focusing was used to isolate a type c cytochrome from photosynthetic membranes of the green sulfur bacterium Chlorobium tepidum. The purified protein showed a molecular weight of 10 kDa according to SDS-PAGE and ESI mass spectrometry. The absorption spectrum in the visible range is typical of a cytochrome with peaks at 420, 525.2 and 554.4 nm. Cleavage by either trypsin or endoproteinase lys-C of the isolated cytochrome combined with tandem mass spectrometry and Edman sequencing yielded a sequence perfectly matching parts of the recently sequenced genome of C. tepidum.
Rémigy
Hervé-W
HW
Division of Biochemistry, Department of Chemistry, University of Crete, 71409, Heraklion, Greece.
Aivaliotis
Michalis
M
Ioannidis
Nikos
N
Jenö
Paul
P
Mini
Thierry
T
Engel
Andreas
A
Jaquinod
Michel
M
Tsiotis
Georgios
G