[Expression of macrophage inflammatory protein-1alpha, a disintegrin-like and metalloproteinase 8 and 12, and CD68 protein in giant cell lesions of jaw and giant cell tumors of long bone].
OBJECTIVE
To detect the expression of macrophage inflammatory protein-1alpha (MIP-1alpha), a disintegrin-like and metalloproteinase (ADAM) 8 and 12 and CD68 protein in giant cell lesions of jaw and giant cell tumors of long bone, and to study their effects on the histogenesis of giant cells in such lesions.


METHODS
MIP-1alpha, ADAM8, ADAM12 and CD68 were detected by immunohistochemistry in 24 paraffin-embedded specimens of central giant cell lesions of jaw and giant cell tumors respectively.


RESULTS
MIP-1alpha positive signal was located in blood vessels and bone. ADAM8, ADAM12 and CD68 positive signals were located in the cell membrane and cytoplasm of all multinucleated giant cells and some round mononuclear cells in the lesions. In addition, some spindle mononuclear stromal cells were positive for ADAM12 in both lesions.


CONCLUSIONS
Multinucleated giant cells probably originate from CD68-postive round mononuclear cells, which are recruited from monocyte-macrophage system by chemokines, such as MIP-1alpha, followed by cell fusion mediated by ADAM8 and ADAM12.
Meng
Xue-mei
XM
Department of Oral Pathology, School of Stomatology, Peking University, Beijing 100081, China.
Yu
Shi-feng
SF
Lu
Min
M
Zheng
Jie
J
Han
Zhi-hui
ZH
D051722:ADAM Proteins / Q000378:metabolism*; D000072199:ADAM12 Protein; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D002462:Cell Membrane / Q000378:metabolism; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D003593:Cytoplasm / Q000378:metabolism; D018212:Giant Cell Tumor of Bone / Q000378:metabolism* / Q000473:pathology; D015726:Giant Cells / Q000378:metabolism; D006101:Granuloma, Giant Cell / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007571:Jaw Diseases / Q000378:metabolism / Q000473:pathology; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D000097382:CD68 Molecule
D051722:ADAM Proteins; D000072199:ADAM12 Protein; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins; D008565:Membrane Proteins; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C000606480:ADAM12 protein, human; C068490:ADAM8 protein, human