Mdv1 interacts with assembled dnm1 to promote mitochondrial division.
The dynamin-related GTPase, Dnm1, self-assembles into punctate structures that are targeted to the outer mitochondrial membrane where they mediate mitochondrial division. Post-targeting, Dnm1-dependent division is controlled by the actions of the WD repeat protein, Mdv1, and the mitochondrial tetratricopeptide repeat-like outer membrane protein, Fis1. Our previous studies suggest a model where at this step Mdv1 functions as an adaptor linking Fis1 with Dnm1. To gain insight into the exact role of the Fis1.Mdv1.Dnm1 complex in mitochondrial division, we performed a structure-function analysis of the Mdv1 adaptor. Our analysis suggests that dynamic interactions between Mdv1 and Dnm1 play a key role in division by regulating Dnm1 self-assembly.
Naylor
Kari
K
Section of Molecular and Cellular Biology, University of California-Davis, Davis, CA 95616, USA.
Ingerman
Elena
E
Okreglak
Voytek
V
Marino
Michael
M
Hinshaw
Jenny E
JE
Nunnari
Jodi
J
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000737:chemistry / Q000502:physiology*; D002455:Cell Division; D003593:Cytoplasm / Q000378:metabolism; D019281:Dimerization; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000737:chemistry; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D008958:Models, Molecular; D016296:Mutagenesis; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000502:physiology*; D013329:Structure-Activity Relationship; D013997:Time Factors
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C429283:MDV1 protein, S cerevisiae; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases; C094774:DNM1 protein, S cerevisiae