title: [Molecular detection of specific HPV types in Condylomata acuminata].
abstract: To detect HPV in genital warts (Condylomata acuminata, CA) for infection rate and association of specific HPV types between males and females, and to provide support for the development of HPV vaccines, we designed HPV type-specific oligonucleotide primers to amplify DNA fragments encoding L1 viral capsule protein. SSP-PCR was conducted in duplication for each CA sample from male and female patients. DNA of TA-cloned HPV was used as positive control, and deionized H2O was used as negative control. A total of 22 clinical samples, 13 from males and 9 from females, was collected from patients diagnosed with CA at hospitals in Beijing and Handan. HPV viral DNA was amplified in all 22 samples analyzed, with 100% detection rate. TA-cloning and sequencing of the PCR products confirmed correct amplification of HPV type-specific fragments. Of the 13 samples from males, 5 were infected with HPV6, 6 with HPV11, and 2 with HPV6 + HPV11. Of the 9 samples from females, 3 were infected with HPV6, 2 with HPV11, and 4 with both HPV6 and HPV11 infection. In addition, high-risk types HPV16, HPV18, HPV33, HPV35, HPV45, HPV54, HPV56 and HPV58 were also detected in 4 female samples that were mixed with cervical cell debris during sample collection. However, no HPV types other than HPV6 and HPV11was detected in all CA-only samples in this study. We have established a sensitive and reliable laboratory procedure for HPV detection and classification. Using the method, we reached 100% detection rate of HPV in the CA samples. Our results confirm that HPV6 and HPV11 are primarily responsible for CA, and there is no specific association of HPV types between warts in males and females.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. xjwu@genetics.ac.cn
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Wu
authors.forename: Jian-Gong
authors.initials: JG
authors.lastname: Ren
authors.forename: Yong-Jiang
authors.initials: YJ
authors.lastname: Liu
authors.forename: Jun-Lian
authors.initials: JL
authors.lastname: Liu
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Tan
authors.forename: Duo-Liang
authors.initials: DL
authors.lastname: Ran
authors.forename: Run-Lin
authors.initials: RL
authors.lastname: Ma
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D002584:Cervix Uteri / Q000821:virology; D003218:Condylomata Acuminata / Q000821:virology*; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D005832:Genital Diseases, Male / Q000821:virology; D052140:Human papillomavirus 11 / Q000235:genetics* / Q000302:isolation & purification; D052163:Human papillomavirus 6 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D002577:Uterine Cervical Diseases / Q000821:virology*; D014623:Vaginal Diseases / Q000821:virology
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral