Synthesis of a small, cysteine-rich, 29 amino acids long peptide in Mycoplasma pneumoniae.
A 205-210 bases long, small RNA (MP200RNA) of Mycoplasma pneumoniae encodes an open reading frame (ORF pmp200) that has the potential to be translated into a 29 amino acids long peptide with nine cysteines. The expression of this peptide in M. pneumoniae was proven indirectly by constructing a gene fusion between the ORF pmp200 and mrfp1, the gene encoding the monomeric red fluorescent protein. The fusion construct was translated in M. pneumoniae. The corresponding fusion protein, with a molecular mass of approximately 35,000 Da, was isolated and the correct sequence was proven by Edman degradation and by mass spectrometry.
Zimmerman
C-U
CU
Zentrum für Molekulare Biologie Heidelberg, Universität Heidelberg, 69120 Heidelberg, Germany.
Herrmann
R
R
D000595:Amino Acid Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D015870:Gene Expression; D008164:Luminescent Proteins; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics*; D000097573:Red Fluorescent Protein
D008164:Luminescent Proteins; D012329:RNA, Bacterial; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein; C408645:MP200 RNA