Microarray profiling of genes differentially expressed during erythroid differentiation of murine erythroleukemia cells.
Murine erythroleukemia (MEL) cells are widely used to study erythroid differentiation thanks to their ability to terminally differentiate in vitro in response to chemical induction. At the molecular level, not much is known of their terminal differentiation apart from activation of adult-type globin gene expression. We examined changes in gene expression during the terminal differentiation of these cells using microarray-based technology. We identified 180 genes whose expression changed significantly during differentiation. The microarray data were analyzed by hierarchical and k-means clustering and confirmed by semi-quantitative RT-PCR. We identified several genes including H1f0, Bnip3, Mgl2, ST7L, and Cbll1 that could be useful markers for erythropoiesis. These genetic markers should be a valuable resource both as potential regulators in functional studies of erythroid differentiation, and as straightforward cell type markers.
Heo
Hyen Seok
HS
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, Korea.
Kim
Ju Hyun
JH
Lee
Young Jin
YJ
Kim
Sung-Hyun
SH
Cho
Yoon Shin
YS
Kim
Chul Geun
CG
D000081:Acetamides / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics* / Q000502:physiology; D016000:Cluster Analysis; D020224:Expressed Sequence Tags / Q000378:metabolism; D020869:Gene Expression Profiling; D005819:Genetic Markers / Q000235:genetics; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000473:pathology; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000235:genetics
D000081:Acetamides; D005819:Genetic Markers; C014026:hexamethylene bisacetamide