[The effects of classic and delayed ischemic preconditioning on the oxidative stress in small bowel autotransplantation model].
Ischemic Preconditioning (IPC) can reduce ischemia/reperfusion injury produced during small bowel transplantation. We investigated the effects of classic and delayed preconditioning on oxidative stress prior to autotransplantation. Total orthotopic intestinal autotransplantation was performed on 18 dogs. In group I (G-I, non-preconditioned) 3-hours cold preservation in University of Wisconsin solution followed by 1-hour of reperfusion. In group II (G-II, classic preconditioned) before this procedure the intestine was preconditioned with 4 cycles of 5 minutes ischemia and 10 minutes reperfusion (IPC protocol). In group III (G-III, delayed preconditioned) on day 1 the animals underwent IPC protocol, and on day 2 autotransplantation was performed. We determined the content of malondialdehyde (MDA), reduced glutathione (GSH), and the activity of superoxide dismutase (SOD) in tissue samples. Our results showed increased lipid peroxidation, slightly elevated GSH level and decreased SOD activity in G-I. In G-II MDA slightly elevated, GSH increased markedly and SOD activity preserved by the end of reperfusion. In G-III GSH significantly increased and SOD activity passed the control activity. Our findings confirmed that both forms of preconditioning could moderate the severity of oxidative stress prior to preservation and auto transplantation. Delayed preconditioning is more effective especially to protect bowel tissue against oxidative injury.
Ferencz
Andrea
A
Pécsi Tudományegyetem Altalános Orvostudományi Kar Sebészeti Oktató es Kutató Intézet. andreaferencz@aok.pte.hu
Rácz
Bóglárka
B
Cserepes
Barbara
B
Roth
Erzsébet
E
D000818:Animals; D004285:Dogs; D005978:Glutathione / Q000032:analysis; D007421:Intestine, Small / Q000201:enzymology / Q000378:metabolism* / Q000637:transplantation*; D019194:Ischemic Preconditioning / Q000379:methods*; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D014182:Transplantation, Autologous
D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione