Enrichment and isolation of anaerobic hydrocarbon-degrading bacteria.
Recent progress in microbiology resulted in the enrichment and isolation of anaerobic bacteria capable of the biodegradation of various hydrocarbons under a variety of electron-accepting conditions. Problems challenging the enrichment and isolation of anaerobic hydrocarbonclastic organisms required new approaches and modifications of conventional microbiological techniques. This chapter summarizes the collective experience accumulated in this area starting from anaerobic sampling precautions and includes all stages of cultivation from the construction of initial incubations to final isolation steps and the evaluation of culture purity.
Davidova
Irene A
IA
Department of Microbiology, University of Oklahoma, Norman 73019, USA.
Suflita
Joseph M
JM
D000473:Alkanes / Q000378:metabolism; D001421:Bacteria, Anaerobic / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D001431:Bacteriological Techniques; D001673:Biodegradation, Environmental; D003470:Culture Media; D019015:Geologic Sediments / Q000382:microbiology; D006838:Hydrocarbons / Q000378:metabolism*; D008858:Microscopy, Phase-Contrast; D012997:Solvents; D014871:Water Microbiology
D000473:Alkanes; D003470:Culture Media; D006838:Hydrocarbons; D012997:Solvents; C059167:2,2,4,4,6,8,8-heptamethylnonane