title: Efficient detection of PrPSc (263K) in human plasma.
abstract: The potential contamination of human blood or plasma with prions, such as variant Creuftzfelt-Jacob disease (vCJD), is becoming a serious problem. In this study, we established a Western blot-based detection method for PrP(Sc) (263K) spiked in plasma. Although plasma contains a large amount of protein, specific detection of a small amount of 263K in plasma could be specifically detected only after ultra-centrifugation followed by heat-denaturation of plasma proteins included in the resulting precipitate, before the digestion with proteinase K.
authors.affiliation: Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Fujita
authors.forename: Takeru
authors.initials: T
authors.lastname: Urayama
authors.forename: Akimasa
authors.initials: A
authors.lastname: Ohmizu
authors.forename: Mikihiro
authors.initials: M
authors.lastname: Yunoki
authors.forename: Shoutaro
authors.initials: S
authors.lastname: Tsuji
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ikuta
mesh_terms: D015153:Blotting, Western / Q000379:methods*; D007562:Creutzfeldt-Jakob Syndrome / Q000175:diagnosis*; D006801:Humans; D018620:PrPSc Proteins / Q000097:blood*; D011489:Protein Denaturation; D014461:Ultracentrifugation
chemical_list: D018620:PrPSc Proteins