Content of 8-hydroxy-2-deoxyguanosine in steroid receptor-positive and receptor-negative breast cancer cells.
The content of DNA damage marker 8-hydroxy-2-deoxyguanosine in 16 receptor-negative and 18 receptor-positive human breast neoplasms was measured by immunohistochemical methods. Positive staining was revealed in 81.3 and 50.0% samples of groups 1 and 2, respectively. The effect of arylhydrocarbon receptor agonist beta-naphthoflavone on the content of 8-hydroxy-2-deoxyguanosine and number of estrogen and progesterone receptors was evaluated in MCF-7 breast cancer cells. The degree of genotoxic damage significantly increased 1 h after combined treatment with estradiol and beta-naphthoflavone (in contrast to individual treatment) and remained practically unchanged in the follow-up period. According to the estrogen effect-switching phenomenon, genotoxic damage can contribute to the development of R(-)-breast cancer.
Bershtein
L M
LM
N. N. Petrov Institute of Oncology; Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Levina
V V
VV
Poroshina
T E
TE
Tsyrlina
E V
EV
D000080242:8-Hydroxy-2'-Deoxyguanosine; D001943:Breast Neoplasms / Q000378:metabolism*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D004958:Estradiol / Q000633:toxicity; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009152:Mutagenicity Tests; D011987:Receptors, Steroid / Q000378:metabolism*; D019324:beta-Naphthoflavone / Q000633:toxicity
D011987:Receptors, Steroid; D004958:Estradiol; D019324:beta-Naphthoflavone; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine