title: Interaction of porphyrin with G-quadruplex structures.
abstract: Isothermal titration calorimetry (ITC) is a sensitive technique for probing bimolecular processes and can provide direct information about the binding affinity and stoichiometry and the key thermodynamic parameters involved. ITC has been used to investigate the interaction of the ligand H2TMPyP to the two DNA quadruplexes, [d(AGGGT)]4 and [d(TGGGGT)]. Analysis of the ITC data reveals that porphyrin/quadruplex binding stoichiometry under saturating conditions is 1:2 for [d(AGGGT)]4 and 2:1 for [d(TGGGGT)], respectively.
authors.affiliation: Dipartimento di Chimica, Universitd di Napoli Federico II, Napoli, Italy.
authors.forename: Eva
authors.initials: E
authors.lastname: Erra
authors.forename: Luigi
authors.initials: L
authors.lastname: Petraccone
authors.forename: Veronica
authors.initials: V
authors.lastname: Esposito
authors.forename: Antonio
authors.initials: A
authors.lastname: Randazzo
authors.forename: Luciano
authors.initials: L
authors.lastname: Mayol
authors.forename: John
authors.initials: J
authors.lastname: Ladbury
authors.forename: Guido
authors.initials: G
authors.lastname: Barone
authors.forename: Concetta
authors.initials: C
authors.lastname: Giancola
mesh_terms: D055592:Biophysical Phenomena; D001703:Biophysics; D002151:Calorimetry; D004247:DNA / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008024:Ligands; D008956:Models, Chemical; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes; D011166:Porphyrins / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors
chemical_list: D008024:Ligands; D009692:Nucleic Acid Heteroduplexes; D011166:Porphyrins; D004247:DNA