title: Enrichment, cultivation, and detection of reductively dechlorinating bacteria.
abstract: Strategies and procedures for enriching, isolating, and cultivating reductively dechlorinating bacteria that use chloroorganic compounds as metabolic electron acceptors from environmental samples are described. Further, nucleic acid-based approaches used to detect and quantify dechlorinator (i.e., Dehalococcoides)-specific genes are presented.
authors.affiliation: School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta 30332-0512, USA.
authors.forename: Frank E
authors.initials: FE
authors.lastname: Löffler
authors.forename: Robert A
authors.initials: RA
authors.lastname: Sanford
authors.forename: Kirsti M
authors.initials: KM
authors.lastname: Ritalahti
mesh_terms: D000085:Acetates / Q000378:metabolism; D001421:Bacteria, Anaerobic / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D006867:Hydrolases / Q000235:genetics; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012989:Soil Pollutants / Q000378:metabolism
chemical_list: D000085:Acetates; D003470:Culture Media; D004269:DNA, Bacterial; D006843:Hydrocarbons, Chlorinated; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D006859:Hydrogen; D006867:Hydrolases; C034507:2-haloacid dehalogenase