title: CD46 is a cellular receptor for all species B adenoviruses except types 3 and 7.
abstract: The 51 human adenovirus serotypes are divided into six species (A to F). Adenovirus serotypes from all species except species B utilize the coxsackie-adenovirus receptor for attachment to host cells in vitro. Species B adenoviruses primarily cause ocular and respiratory tract infections, but certain serotypes are also associated with renal disease. We have previously demonstrated that adenovirus type 11 (species B) uses CD46 (membrane cofactor protein) as a cellular receptor instead of the coxsackie-adenovirus receptor (A. Segerman et al., J. Virol. 77:9183-9191, 2003). In the present study, we found that transfection with human CD46 cDNA rendered poorly permissive Chinese hamster ovary cells more permissive to infection by all species B adenovirus serotypes except adenovirus types 3 and 7. Moreover, rabbit antiserum against human CD46 blocked or efficiently inhibited all species B serotypes except adenovirus types 3 and 7 from infecting human A549 cells. We also sequenced the gene encoding the fiber protein of adenovirus type 50 (species B) and compared it with the corresponding amino acid sequences from selected serotypes, including all other serotypes of species B. From the results obtained, we conclude that CD46 is a major cellular receptor on A549 cells for all species B adenoviruses except types 3 and 7.
authors.affiliation: Department of Virology, Umeå University, SE-901 85 Umeå, Sweden.
authors.forename: Marko
authors.initials: M
authors.lastname: Marttila
authors.forename: David
authors.initials: D
authors.lastname: Persson
authors.forename: Dan
authors.initials: D
authors.lastname: Gustafsson
authors.forename: M Kathryn
authors.initials: MK
authors.lastname: Liszewski
authors.forename: John P
authors.initials: JP
authors.lastname: Atkinson
authors.forename: Göran
authors.initials: G
authors.lastname: Wadell
authors.forename: Niklas
authors.initials: N
authors.lastname: Arnberg
mesh_terms: D000260:Adenoviruses, Human / Q000145:classification / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000502:physiology; D016466:CHO Cells; D002460:Cell Line; D017124:Conserved Sequence; D006224:Cricetinae; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017433:Protein Structure, Secondary; D011991:Receptors, Virus / Q000737:chemistry / Q000502:physiology*; D020545:Respiratory Mucosa; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D015703:Antigens, CD; D011991:Receptors, Virus