title: Development of a rapid method for simultaneous recovery of diverse microbes in drinking water by ultrafiltration with sodium polyphosphate and surfactants.
abstract: The ability to simultaneously concentrate diverse microbes is an important consideration for sample collection methods that are used for emergency response and environmental monitoring when drinking water may be contaminated with an array of unknown microbes. This study focused on developing a concentration method using ultrafilters and different combinations of a chemical dispersant (sodium polyphosphate [NaPP]) and surfactants. Tap water samples were seeded with bacteriophage MS2, Escherichia coli, Enterococcus faecalis, Cryptosporidium parvum, 4.5-microm microspheres, Salmonella enterica serovar Typhimurium, Bacillus globigii endospores, and echovirus 1. Ten-liter tap water samples were concentrated to approximately 250 ml in 12 to 42 min, depending on the experimental condition. Initial experiments indicated that pretreating filters with fetal bovine serum or NaPP resulted in an increase in microbe recovery. The addition of NaPP to the tap water samples resulted in significantly higher microbe and microsphere recovery efficiencies. Backflushing of the ultrafilter was found to significantly improve recovery efficiencies. The effectiveness of backflushing was improved further with the addition of Tween 80 to the backflush solution. The ultrafiltration method developed in this study, incorporating the use of NaPP pretreatment and surfactant solution backflushing, was found to recover MS2, C. parvum, microspheres, and several bacterial species with mean recovery efficiencies of 70 to 93%. The mean recovery efficiency for echovirus 1 (49%) was the lowest of the microbes studied for this method. This research demonstrates that ultrafiltration can be effective for recovering diverse microbes simultaneously in tap water and that chemical dispersants and surfactants can be beneficial for improving microbial recovery using this technique.
authors.affiliation: Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Parasitic Diseases, 4770 Buford Highway, Mail Stop F-36, Atlanta, GA 30341-3724, USA. vhill@cdc.gov
authors.forename: Vincent R
authors.initials: VR
authors.lastname: Hill
authors.forename: Amy L
authors.initials: AL
authors.lastname: Polaczyk
authors.forename: Donghyun
authors.initials: D
authors.lastname: Hahn
authors.forename: Jothikumar
authors.initials: J
authors.lastname: Narayanan
authors.forename: Theresa L
authors.initials: TL
authors.lastname: Cromeans
authors.forename: Jacquelin M
authors.initials: JM
authors.lastname: Roberts
authors.forename: James E
authors.initials: JE
authors.lastname: Amburgey
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D016785:Cryptosporidium parvum / Q000302:isolation & purification; D005618:Fresh Water / Q000382:microbiology / Q000469:parasitology / Q000821:virology; D011122:Polyphosphates / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*; D013997:Time Factors; D014462:Ultrafiltration / Q000379:methods; D014780:Viruses / Q000302:isolation & purification; D018508:Water Purification / Q000379:methods*; D014881:Water Supply
chemical_list: D011122:Polyphosphates; D011136:Polysorbates; D013501:Surface-Active Agents