title: Surface modification and initial adhesion events for intestinal epithelial cells.
abstract: Rapid resealing of the mucosal epithelia is imperative following injuries to the small intestine because the mucosa is responsible for the adsorption of nutrients as well as providing a barrier to noxious agents present in the lumen. Tissue engineering may provide a possible solution for treating intestinal erosions, ulcerations, inflammatory bowel disease, and infection. Cell-biomaterial interaction is a critical component in tissue engineering that can determine the success of the tissue construct. Cell-biomaterial interactions can be enhanced by various types of surface modification, which promote integrin ligation leading to increased cell function. In order to relate the effect of surface adhesion molecules to signaling events and macroscopic cell response, an intestinal epithelial cell line, IEC-6, was plated on fibronectin (receptor-mediated) and poly-L-lysine (non-specific) surfaces. Focal adhesion kinase (FAK) phosphorylation, cell spreading, and cell adhesion strength were measured. Results showed increases in FAK phosphorylation generally corresponded to increases in cell spreading and adhesion strength for IEC-6 cells. Therefore, in a simplified system, initial adhesion and signaling mechanisms appeared to correspond to subsequent physical responses in IEC-6 cells relevant to tissue engineering applications.
authors.affiliation: Department of Bioengineering, University of California-Los Angeles, Los Angeles, CA, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Hagerman
authors.forename: S H H
authors.initials: SH
authors.lastname: Chao
authors.forename: J C Y
authors.initials: JC
authors.lastname: Dunn
authors.forename: B M
authors.initials: BM
authors.lastname: Wu
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D048430:Cell Shape; D002478:Cells, Cultured; D005353:Fibronectins / Q000494:pharmacology; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology*; D007421:Intestine, Small / Q000166:cytology; D010766:Phosphorylation; D011107:Polylysine / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D005353:Fibronectins; D011107:Polylysine; D051416:Focal Adhesion Protein-Tyrosine Kinases