Determination of bovine serum albumin by a resonance light-scattering technique with the mixed-complex La(Phth)(phen)(3+).
A mixed-complex of La(Phth)(phen)(3+) was synthesized. Resonance light-scattering characteristics of interaction between La(Phth)(phen)(3+) with bovine serum albumin (BSA) were studied. When BSA was added, aggregation of La(Phth)(phen)(3+) on the molecular surface of bovine serum albumin occurred in the pH 5.5-6.3, resulted in an enhanced resonance light-scattering (RLS) peak at 360 nm. The intensity of resonance light-scattering was found to be proportional to the concentration of BSA.
Li
Ling
L
Faculty of Chemistry and Material Science, Hubei University, Wu Han 430062, PR China. waitingll@yahoo.com
Song
Gongwu
G
Fang
Guangrong
G
D000818:Animals; D002138:Calibration; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008027:Light; D009942:Organometallic Compounds / Q000737:chemistry*; D012015:Reference Standards; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013050:Spectrometry, Fluorescence
C510633:(1,10-phenanthroline)(2-phthalic acid)lanthanum(III); D007202:Indicators and Reagents; D009942:Organometallic Compounds; D012710:Serum Albumin, Bovine