Electrochemical study on the behavior of Morin and its interaction with DNA.
Voltammetric behavior of Morin was studied in 0.1M HAc-NaAc+50mM KCl (pH 3.4) solution at glassy carbon electrode (GCE) using cyclic voltammetry (CV). Morin showed an irreversible anodic peak at 0.720 V in CV which was involving two electrons and two protons. Also, the interaction of Morin with double-stranded calf thymus DNA (ctDNA) was studied by CV at GCE with an irreversible electrochemical equation. As a result of reaction with ctDNA, the voltammetric peak of Morin was a position shift and the peak current decreased. The diffusion coefficients of both free and binding Morin (D(f)=1.1,086 x 10(-7)cm(2)s(-1) and D(b)=8.2,544 x 10(-9)cm(2)s(-1)), binding constant (K=1.7,765 x 10(7)cm(3)mol(-1)), and binding site size (s=0.8,510) of the Morin-DNA complex were obtained simultaneously by non-linear fit analysis. The results demonstrate that Morin can bind to ctDNA in 0.1M HAc-NaAc+50mM KCl (pH 3.4) solution and the ring B of Morin intercalates between the DNA base pairs.
Kang
Jingwan
J
Department of Chemistry, Northwest Normal University, Lanzhou 730070, PR China. jwkang2nwnu.edu.cn
Li
Zhifeng
Z
Lu
Xiaoquan
X
D000465:Algorithms; D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D020029:Base Pairing; D001665:Binding Sites; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D005419:Flavonoids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012313:RNA / Q000737:chemistry; D047309:Flavones
D000975:Antioxidants; D004247:DNA; D005419:Flavonoids; D012313:RNA; C008548:morin; D047309:Flavones