Noninvasive method of immortalized neural stem-like cell transplantation in an experimental model of Huntington's disease.
A loss of neostriatal neurons is a characteristic of Huntington's disease (HD), and neural tissue transplantation has been performed directly into the striatum. Since the neural stem cells have ability to migrate into the lesion site, we administered immortalized neural stem-like cells (NSC) into the ventricle or via a tail vein following unilateral intrastriatal quinolinic acid lesioning in Sprague-Dawley rats. To identify transplanted NSC, cells were encoded with lac Z and beta-galactosidase histochemistry was performed. lac Z+ cells were detected in the lesioned striatum but tissue damage or tumor formation was not observed. This study shows that NSC migrate into the striatum, either from ventricle or from the systemic circulation, providing less invasive routes for stem cell application in HD.
Lee
Soon-Tae
ST
Department of Neurology, Seoul National University Hospital, Neuroscience Research Institute of SNUMRC, 28 Yongondong, Chongnoku, Seoul 110-744, South Korea.
Park
Jung-Eun
JE
Lee
Kyungmi
K
Kang
Lami
L
Chu
Kon
K
Kim
Seung U
SU
Kim
Manho
M
Roh
Jae-Kyu
JK
D000818:Animals; D002461:Cell Line, Transformed / Q000637:transplantation*; D002465:Cell Movement; D006801:Humans; D006816:Huntington Disease / Q000139:chemically induced / Q000628:therapy*; D007150:Immunohistochemistry; D007165:Immunosuppression Therapy; D007276:Injections, Intraventricular; D007763:Lac Operon / Q000235:genetics; D008297:Male; D009474:Neurons / Q000637:transplantation*; D017378:Quinolinic Acid / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods*; D013238:Stereotaxic Techniques
D017378:Quinolinic Acid