Antifungal activity of DNA-lipid complexes and DNA-lipid films against Candida species.
In this study amphiphilic lipids, DNA-lipid complexes, and DNA-lipid films were prepared, and their antifungal activity against Candida species was examined. The amphiphilic lipids were synthesized from a reaction of glycine or L-alanine with n-alkyl alcohol in the presence of p-toluene sulfonic acid. DNA-lipid complexes, which were prepared by the simple mixing of DNA and amphiphilic lipids, were insoluble in water. Self-standing, water-insoluble DNA-lipid films were prepared by casting the DNA-lipid complexes from a chloroform/ethanol solution. The antifungal activities of the lipids and DNA-lipid complexes against the Candida species were evaluated by minimum inhibitory concentrations (MICs); those of DNA-lipid films were evaluated by the disk diffusion method. The seven kinds of lipids, DNA-lipid complexes, and DNA-lipid films showed antifungal activity, and no differences were seen in the antifungal activities between glycine and L-alanine derivatives. The lipids, DNA-lipid complexes, and DNA-lipid films, which have shorter alkyl chain length in lipids, showed antifungal activity against all Candida species. However, the effect of antifungal activity against Candida species decreased with increased alkyl chain length in lipids. In this study, it was found that lipids, DNA-lipid complexes, and films with a decyl or dodecyl group exhibit more favorable antifungal activity.
Inoue
Y
Y
Fukuoka College of Health Sciences, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan. yusuke@college.fdcnet.ac.jp
Fukushima
T
T
Hayakawa
T
T
Ogura
R
R
Kaminishi
H
H
Miyazaki
K
K
Okahata
Y
Y
D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002175:Candida / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D041221:Candida glabrata / Q000187:drug effects; D041022:Candida tropicalis / Q000187:drug effects; D004247:DNA / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D046911:Macromolecular Substances; D008422:Materials Testing; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests
D000935:Antifungal Agents; D001672:Biocompatible Materials; D008055:Lipids; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D004247:DNA