[Use of allogenic acellular dermal matrix combined with autologous epidermal cells for the repair of tissue defect].
OBJECTIVE
To investigate a method for the repair of tissue defect.


METHODS
Allogenic acellular dermal matrixes (ADM) were implanted to full-thickness skin defects made on the dorsa of rats. Two weeks later, autologous suspended epidermal cells were transplanted on to the surface of vascularized ADM. Respectively, neoepidermis was macroscopically observed 2, 3, 5 weeks after grafting, and samples were taken to make routine paraffin sections for microscopical examination, and immunohistochemical staining for type IV collagen was also performed.


RESULTS
The vascularized ADM could support proliferation and differentiation of epidermal cells, and also could promote the formation of dermal-epidermal junction. Suspended epidermal cells in an artificial culture system in vivo could develop into mature epidermis. The reconstructed skin not only looked like the normal one in appearance in which hair was removed, but also revealed a better function.


CONCLUSIONS
Full-thickness skin defect can be repaired by transplanting autologous epidermal cell suspension on to vascularized ADM.
Zuo
Jin-hua
JH
Department of Oral and Maxillofacial Surgery, Affiliated Hospital, Binzhou Medical College, Binzhou Shandong 256603, China.
Li
Jin-rong
JR
Li
Wu-xiu
WX
Yang
You-cheng
YC
Wu
Shu-hua
SH
Lü
Zeng-hua
ZH
D000818:Animals; D017690:Cell Transplantation; D020405:Dermis / Q000166:cytology*; D000078404:Epidermal Cells; D005109:Extracellular Matrix; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries*; D016038:Skin Transplantation / Q000379:methods; D017695:Soft Tissue Injuries / Q000601:surgery*; D013535:Suspensions; D023822:Tissue Engineering; D014183:Transplantation, Heterologous; D014945:Wound Healing
D013535:Suspensions