High sensitive analysis of rat serum bile acids by liquid chromatography/electrospray ionization tandem mass spectrometry.
Sensitive liquid chromatography (LC)/electrospray ionization (ESI) tandem mass spectrometry (MS) can be used to analyze the bile acid composition of rat serum. This method can analyze eight common bile acids and their glycine and taurine conjugates in 100 microl rodent serum by gradient elution on a reversed-phase column using a mixture of 20mM ammonium acetate buffer (pH 8.0), acetonitrile and methanol as a mobile phase. Selected reaction monitoring analysis under negative ion detection mode allowed the achievement of a high sensitive assay with a simple solid phase extraction using an ODS cartridge column. We used this method to investigate the effect of a one-day fast on the concentration and composition of serum bile acids in rats. The results suggested that the method described here is useful for the dynamic analysis of serum bile acids in rats.
Ando
Masayuki
M
Niigata University of Pharmacy and Applied Life Sciences, 5-13-2 Kamishinei-cho, Niigata 950-2081, Japan.
Kaneko
Tohru
T
Watanabe
Ritsuko
R
Kikuchi
Satoshi
S
Goto
Takaaki
T
Iida
Takashi
T
Hishinuma
Takanori
T
Mano
Nariyasu
N
Goto
Junichi
J
D000818:Animals; D001647:Bile Acids and Salts / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005998:Glycine / Q000737:chemistry; D007202:Indicators and Reagents; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013654:Taurine / Q000737:chemistry
D001647:Bile Acids and Salts; D007202:Indicators and Reagents; D013654:Taurine; D005998:Glycine