title: Characterization of Parvovirus B19 genotype 2 in KU812Ep6 cells.
abstract: An infectious parvovirus B19 (B19V) genotype 2 variant was identified as a high-titer contaminant in a human plasma donation. Genome analysis revealed a 138-bp insertion within the p6 promoter. The inserted sequence was represented by an additional 30 bp from the end of the inverted terminal repeat adjacent to a 108-bp element found also, in inverted orientation, at the extreme right end of the unique sequence of the genome. However, despite the profound variations in the promoter region, the pattern of gene expression and DNA replication did not differ between genotype 1 and genotype 2 in permissive erythroid KU812Ep6 cells. Capsid proteins of both genotypes differ in their amino acid sequences. However, equivalent kinetics of virus inactivation at 56 degrees C or pH 4 indicated a comparable physicochemical stability of virus capsids. Sera from six individuals infected by B19V genotype 1 were investigated on cross-neutralization of B19V genotype 2 in vitro. Similar neutralization of both B19V genotypes was observed in sera from three individuals, while the sera from three other individuals showed weaker cross-neutralization for genotype 2. In conclusion, the in vitro replication characteristics and physical stability of B19V capsids are very similar between human parvovirus B19 genotypes 1 and 2, and cross-neutralization indicates a close antigenic relation of genotypes 1 and 2.
authors.affiliation: Paul-Ehrlich-Institut, Langen, Germany. blujo@pei.de
authors.forename: Johannes
authors.initials: J
authors.lastname: Blümel
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Eis-Hübinger
authors.forename: Albert
authors.initials: A
authors.lastname: Stühler
authors.forename: Claudia
authors.initials: C
authors.lastname: Bönsch
authors.forename: Matthias
authors.initials: M
authors.lastname: Gessner
authors.forename: Johannes
authors.initials: J
authors.lastname: Löwer
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D017124:Conserved Sequence; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000097:blood; D016732:Parvovirus B19, Human / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014766:Viremia / Q000097:blood
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D012367:RNA, Viral