title: Tumor rejection by the poliovirus receptor family ligands of the DNAM-1 (CD226) receptor.
abstract: The poliovirus receptor CD155 and its family member CD112 (nectin-2) are the ligands for the activating cell-surface receptor DNAM-1 on CD8+ T cells and natural killer (NK) cells. Here, we demonstrate that, whereas the RMA tumor grew in syngeneic mice, DNAM-1 ligand-transduced RMA was rejected, in which CD8+ T cells and NK cells played an essential role. Importantly, CD8+ memory cytotoxic T cells to parental RMA were generated in these mice. We found that DNAM-1 was also expressed on CD8alpha+, rather than CD8alpha-, dendritic cells (DCs). Cross-linking DNAM-1 induced maturation of CD8alpha+ DCs. Antigen presentation by these stimulated DCs drove Th1 cells. Moreover, the rejection of DNAM-1 ligand-transduced RMA was canceled in CD4+ T-cell-depleted and major histocompatibility complex class II-deficient mice. Taken together, these results suggest that DNAM-1 ligands stimulate innate immunity by CD8alpha+ DCs as well as NK cells, which efficiently prime cell-mediated tumor-specific immunity.
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1, Ten-nodai, Tsukuba, Ibaraki 305-8575, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Tahara-Hanaoka
authors.forename: Kazuko
authors.initials: K
authors.lastname: Shibuya
authors.forename: Hirayasu
authors.initials: H
authors.lastname: Kai
authors.forename: Akitomo
authors.initials: A
authors.lastname: Miyamoto
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Morikawa
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ohkochi
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Honda
authors.forename: Akira
authors.initials: A
authors.lastname: Shibuya
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D007156:Immunologic Memory; D008024:Ligands; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000276:immunology*; D011991:Receptors, Virus / Q000276:immunology*; D000098862:T Lineage-Specific Activation Antigen 1
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; D008024:Ligands; D008565:Membrane Proteins; D011991:Receptors, Virus; C066100:poliovirus receptor