title: Focal adhesion molecules as potential target of lead toxicity in NRK-52E cell line.
abstract: In this study, we investigated the influence of inorganic lead (Pb(II)), an environmental pollutant having nephrotoxic action, on the focal adhesion (FA) organization of a rat kidney epithelial cell line (NRK-52E). In particular, we evaluated the effects of the metal on the recruitment of paxillin, focal adhesion kinase, vinculin and cytoskeleton proteins at the FAs complexes. We provided evidences that, in proliferating NRK-52E cell cultures, low concentrations of Pb(II) affect the cell adhesive ability and stimulate the disassembly of FAs, thus inhibiting the integrin-activated signalling. These effects appeared to be strictly associated to the Pb-induced arrest of cell cycle at G0/G1 phase also proved in this cell line.
authors.affiliation: Unit of Biochemistry, School of Medicine, University of Brescia, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Giuliani
authors.forename: Francesca
authors.initials: F
authors.lastname: Bettoni
authors.forename: Daria
authors.initials: D
authors.lastname: Leali
authors.forename: Fausta
authors.initials: F
authors.lastname: Morandini
authors.forename: Pietro
authors.initials: P
authors.lastname: Apostoli
authors.forename: Piergiovanni
authors.initials: P
authors.lastname: Grigolato
authors.forename: Bruno Mario
authors.initials: BM
authors.lastname: Cesana
authors.forename: Maria Francesca
authors.initials: MF
authors.lastname: Aleo
mesh_terms: D000199:Actins / Q000187:drug effects; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000378:metabolism*; D004785:Environmental Pollutants / Q000633:toxicity*; D004847:Epithelial Cells / Q000737:chemistry / Q000187:drug effects; D022001:Focal Adhesions / Q000187:drug effects*; D007668:Kidney / Q000166:cytology / Q000187:drug effects*; D007854:Lead / Q000633:toxicity*; D008870:Microtubules / Q000187:drug effects; D051381:Rats
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D004785:Environmental Pollutants; D007854:Lead; C029891:lead chloride