Effects of canine serum collected from dogs at different estrous cycle stages on in vitro nuclear maturation of canine oocytes.
Canine oocytes are ovulated at prophase of the first meiotic division and undergo maturation in the distal part of the oviduct for at least 48-72 h. Because of these differences from other domestic mammals, the efficiency of in vitro maturation (IVM) of canine oocyte is very low. The present study was conducted to evaluate the effects of canine serum on IVM of canine oocytes recovered from ovaries in various reproductive states (follicular, luteal or anestrous stages). Oocytes were recovered by mincing ovaries from bitches presented for ovariohysterectomy at various stages of the estrous cycle. Heat-inactivated canine serum was prepared with blood taken from dogs at the anestrous, estrous or diestrous stage of the estrous cycle as determined by progesterone concentration and vaginal cytology. Oocytes were cultured for 72 h in tissue culture medium (TCM)-199 supplemented with 10% canine anestrous, estrous or diestrous serum or fetal bovine serum (FBS) (experiment 1), or supplemented with 0 (control), 5%, 10% or 20% canine estrous serum (experiment 2). In experiment 1, IVM of oocytes collected at the follicular stage of the estrous cycle to metaphase II (MII) stage was higher (p < 0.05) with canine estrous serum (14.2%) than with canine anestrous (5.2%) or diestrous serum (6.3%), FBS (2.2%) or in the control (2.2%). In experiment 2, oocytes collected at the follicular stage of the estrous cycle cultured in TCM-199 with 10% canine estrous serum showed a higher maturation rate to MII stage (13.5%, p < 0.05) compared with those cultured with 5% (1.3% MII) or 20% canine estrous serum (5.1% MII) or the control (2.7% MII). In conclusion, our results demonstrate that supplementing culture medium with 10% canine estrous serum improves IVM of canine follicular stage oocytes.
Oh
Hyun Ju
HJ
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Korea.
Fibrianto
Yuda Heru
YH
Kim
Min Kyu
MK
Jang
Goo
G
Hossein
M Shamim
MS
Kim
Hye Jin
HJ
Kang
Sung Keun
SK
Lee
Byeong Chun
BC
Hwang
Woo Suk
WS
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004285:Dogs / Q000502:physiology*; D030762:Estrous Cycle / Q000502:physiology*; D005187:Fallopian Tubes; D005260:Female; D066298:In Vitro Techniques; D008540:Meiosis / Q000502:physiology*; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development
D003470:Culture Media