[Therapeutic neovascularization with autologous bone marrow CD34+ cells transplantation in hindlimb ischemia].
OBJECTIVE
To explore whether transplantation of autologous bone marrow stem cells might augment angiogenesis and collateral vessel formation in a canine model of hindlimb ischemia.


METHODS
CD34(+) stem cells were centrifugation through Ficoll and an immune magnetic cell sorting system from bone marrow (20 ml) of canine (n = 5) and induced into endothelial cells with VEGF in vitro, and expression of von Willebrand factor. Bilateral hindlimb ischemia was surgically induced in canines and Dil fluorescence labeled autologous stem cells were transplanted into the ischemic tissues.


RESULTS
Four weeks after transplantation, fluorescence microscopy revealed that transplanted cells were incorporated into the capillary network among preserved skeletal myocytes. The stem cells transplanted group had more angiographically detectable collateral vessels, a higher capillary density (12.0 +/- 2.8 vs. 5.0 +/- 1.6 per field; t = 4.17 P < 0.05) and a higher ABI (0.58 +/- 0.14 vs. 0.32 +/- 0.11; t = 2.95, P < 0.05).


CONCLUSIONS
Direct local transplantation of autologous bone marrow CD34(+) stem cells seems to be a useful strategy for therapeutic neovascularization in ischemic tissues in adults, consistent with "therapeutic vasculogenesis."
Zhang
Hong-kun
HK
Department of Vascular Surgery, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. doczhk@yahoo.com.cn
Zhang
Nan
N
Wu
Li-hua
LH
Jin
Wei
W
Li
Ming
M
Feng
Hua
H
Zhao
Hai-ge
HG
Chen
Xu-dong
XD
Yang
Chun-hu
CH
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D002454:Cell Differentiation; D004195:Disease Models, Animal; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D006614:Hindlimb / Q000098:blood supply*; D007511:Ischemia / Q000628:therapy*; D008297:Male; D018919:Neovascularization, Physiologic
D018952:Antigens, CD34