Attenuation of renal ischemia-reperfusion injury by proanthocyanidin-rich extract from grape seeds.
The effects of proanthocyanidin-rich extract in rats subjected to renal ischemia-reperfusion were examined. Proanthocyanidin-rich extract, which is prepared from grape seeds (Vitis vinifera L.), was given orally at doses of 5 and 10 mg/kg body weight/d for 20 consecutive days prior to ischemia-reperfusion. Administration of proanthocyanidin-rich extract attenuated renal dysfunction, as indicated by serum urea nitrogen and creatinine levels. Additionally, in the ischemic-reperfused kidneys, increased levels of thiobarbituric acid (TBA)-reactive substance and alterations of antioxidant enzyme activities such as superoxide dismutase, catalase and glutathione peroxidase (GSH-Px) were observed. Proanthocyanidin-rich extract-treated groups showed significantly reduced renal TBA-reactive substance levels and enhanced catalase and GSH-Px activities. These results suggest that proanthocyanidin-rich extract has protective effects against ischemia-reperfusion-induced renal damage associated with oxidative stress.
Nakagawa
Takako
T
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
Yokozawa
Takako
T
Satoh
Akiko
A
Kim
Hyun Young
HY
D000818:Animals; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000098:blood supply*; D008297:Male; D018384:Oxidative Stress; D010936:Plant Extracts / Q000008:administration & dosage*; D044945:Proanthocyanidins / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D012639:Seeds / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D027843:Vitis / Q000737:chemistry*
D010936:Plant Extracts; D044945:Proanthocyanidins; D017392:Thiobarbituric Acid Reactive Substances; C013221:proanthocyanidin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase