Impact of different in vitro electron donor/acceptor conditions on potential chemolithoautotrophic communities from marine pelagic redoxclines.
Anaerobic or microaerophilic chemolithoautotrophic bacteria have been considered to be responsible for CO2 dark fixation in different pelagic redoxclines worldwide, but their involvement in redox processes is still not fully resolved. We investigated the impact of 17 different electron donor/acceptor combinations in water of pelagic redoxclines from the central Baltic Sea on the stimulation of bacterial CO2 dark fixation as well as on the development of chemolithoautotrophic populations. In situ, the highest CO2 dark fixation rates, ranging from 0.7 to 1.4 micromol liter(-1) day(-1), were measured directly below the redoxcline. In enrichment experiments, chemolithoautotrophic CO2 dark fixation was maximally stimulated by the addition of thiosulfate, reaching values of up to 9.7 micromol liter(-1) CO2 day(-1). Chemolithoautotrophic nitrate reduction proved to be an important process, with rates of up to 33.5 micromol liter(-1) NO3(-) day(-1). Reduction of Fe(III) or Mn(IV) was not detected; nevertheless, the presence of these potential electron acceptors influenced the development of stimulated microbial assemblages. Potential chemolithoautotrophic bacteria in the enrichment experiments were displayed on 16S ribosomal complementary DNA single-strand-conformation polymorphism fingerprints and identified by sequencing of excised bands. Sequences were closely related to chemolithoautotrophic Thiomicrospira psychrophila and Maorithyas hadalis gill symbiont (both Gammaproteobacteria) and to an uncultured nitrate-reducing Helicobacteraceae bacterium (Epsilonproteobacteria). Our data indicate that this Helicobacteraceae bacterium could be of general importance or even a key organism for autotrophic nitrate reduction in pelagic redoxclines.
Labrenz
Matthias
M
Leibniz-Institut für Ostseeforschung Warnemünde, Seestrasse 15, 18119 Rostock-Warnemünde, Germany. matthias.labrenz@io-warnemuende.de
Jost
Günter
G
Pohl
Christa
C
Beckmann
Sabrina
S
Martens-Habbena
Willm
W
Jürgens
Klaus
K
D002245:Carbon Dioxide / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D003624:Darkness; D017753:Ecosystem; D004579:Electron Transport; D020565:Epsilonproteobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D013885:Thiosulfates / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D013885:Thiosulfates; D002245:Carbon Dioxide