title: Rhizobium leguminosarum biovar viciae symbiotic hydrogenase activity and processing are limited by the level of nickel in agricultural soils.
abstract: Analysis of levels of hydrogenase processing and activity in Rhizobium leguminosarum biovar viciae bacteroids from pea (Pisum sativum) plants showed that the oxidation of nitrogenase-evolved hydrogen is limited by the availability of nickel in agricultural soils. This limitation was overcome by using an inoculant strain engineered for higher hydrogenase expression.
authors.affiliation: Department of Biotechnology, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
authors.forename: Ana-Claudia
authors.initials: AC
authors.lastname: Ureta
authors.forename: Juan
authors.initials: J
authors.lastname: Imperial
authors.forename: Tomás
authors.initials: T
authors.lastname: Ruiz-Argüeso
authors.forename: Jose M
authors.initials: JM
authors.lastname: Palacios
mesh_terms: D000383:Agriculture; D015964:Gene Expression Regulation, Bacterial; D006864:Hydrogenase / Q000235:genetics / Q000378:metabolism*; D009532:Nickel / Q000494:pharmacology*; D018532:Pisum sativum / Q000254:growth & development / Q000382:microbiology; D016961:Rhizobium leguminosarum / Q000201:enzymology* / Q000235:genetics; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D013559:Symbiosis; D014131:Trace Elements / Q000494:pharmacology*
chemical_list: D012987:Soil; D014131:Trace Elements; D009532:Nickel; D006864:Hydrogenase