title: A polypyrrole protein microarray for antibody-antigen interaction studies using a label-free detection process.
abstract: Protein microarray is a promising technology that should combine rapidity and easy use with high throughput and versatility. This article describes a method in which an electrocopolymerization process is employed to graft biological molecules on to a chip so that surface plasmon resonance imaging may be used to detect molecular interactions. Copolymerization of pyrrole-modified protein and pyrrole is an efficient grafting process which immobilizes molecules at defined positions on a gold surface. Surface plasmon resonance imaging is an optical technique that allows real-time simultaneous detection of molecular interactions on a large number of spots without labeling. This method was successfully used to analyze antibody-antigen interactions. This illustrates its high specificity and good sensitivity and demonstrates its suitability for biological studies.
authors.affiliation: CREAB, UMR 5819 (CEA, CNRS, UJF), CEA-G/DRFMC, 17 rue des Martyrs, 38054 Grenoble, France.
authors.forename: Ludivine
authors.initials: L
authors.lastname: Grosjean
authors.forename: Boutheina
authors.initials: B
authors.lastname: Cherif
authors.forename: Emilie
authors.initials: E
authors.lastname: Mercey
authors.forename: André
authors.initials: A
authors.lastname: Roget
authors.forename: Yves
authors.initials: Y
authors.lastname: Levy
authors.forename: Patrice Noel
authors.initials: PN
authors.lastname: Marche
authors.forename: Marie-Bernadette
authors.initials: MB
authors.lastname: Villiers
authors.forename: Thierry
authors.initials: T
authors.lastname: Livache
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D002623:Chemistry Techniques, Analytical; D006063:Chorionic Gonadotropin / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D009113:Muramidase / Q000276:immunology; D011108:Polymers; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011758:Pyrroles; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D006063:Chorionic Gonadotropin; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D009113:Muramidase