title: ERK/MAPK regulates the Kv4.2 potassium channel by direct phosphorylation of the pore-forming subunit.
abstract: Kv4.2 is the primary pore-forming subunit encoding A-type currents in many neurons throughout the nervous system, and it also contributes to the transient outward currents of cardiac myocytes. A-type currents in the dendrites of hippocampal CA1 pyramidal neurons are regulated by activation of ERK/MAPK, and Kv4.2 is the likely pore-forming subunit of that current. We showed previously that Kv4.2 is directly phosphorylated at three sites by ERK/MAPK (T602, T607, and S616). In this study we determined whether direct phosphorylation of Kv4.2 by ERK/MAPK is responsible for the regulation of the A-type current observed in neurons. We made site-directed mutants, changing the phosphosite serine (S) or threonine (T) to aspartate (D) to mimic phosphorylation. We found that the T607D mutation mimicked the electrophysiological changes elicited by ERK/MAPK activation in neurons: a rightward shift of the activation curve and an overall reduction in current compared with wild type (WT). Surprisingly, the S616D mutation caused the opposite effect, a leftward shift in the activation voltage. K(+) channel-interacting protein (KChIP)3 ancillary subunit coexpression with Kv4.2 was necessary for the T607D effect, as the T607D mutant when expressed in the absence of KChIP3 was not different from WT Kv4.2. These data suggest that direct phosphorylation of Kv4.2 at T607 is involved in the dynamic regulation of the channel function by ERK/MAPK and an interaction of the primary subunit with KChIP is also necessary for this effect. Overall these studies provide new insights into the structure-function relationships for MAPK regulation of membrane ion channels.
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, Texas, USA. schrader@tulane.edu
authors.forename: Laura A
authors.initials: LA
authors.lastname: Schrader
authors.forename: Shari G
authors.initials: SG
authors.lastname: Birnbaum
authors.forename: Brian M
authors.initials: BM
authors.lastname: Nadin
authors.forename: Yajun
authors.initials: Y
authors.lastname: Ren
authors.forename: Duy
authors.initials: D
authors.lastname: Bui
authors.forename: Anne E
authors.initials: AE
authors.lastname: Anderson
authors.forename: J David
authors.initials: JD
authors.lastname: Sweatt
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009865:Oocytes; D010766:Phosphorylation; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D051672:Shal Potassium Channels / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D021122:Protein Subunits; D051672:Shal Potassium Channels; D020929:Mitogen-Activated Protein Kinase Kinases