[Effects of TGFbeta1 on the transcriptional activity of SP1, AP1 and Smad3-Smad4 in lung fibroblasts].
OBJECTIVE
To investigate the effects of TGFbeta1 on the transcriptive activity of SP1, AP1 and Smad3-Smad4 in rat lung fibroblasts(RLF).


METHODS
RLFs were isolated and cultured and then treated with 10 microg/L TGFbeta1. The transcriptive activity of SP1, AP1 and Smad3-Smad4 and the expression of plasminogen activator inhibitor I(PAI-1) and collagen I were detected by means of electrophoretic mobility shift assay (EMSA), Western blot and immunohistochemical staining.


RESULTS
After treatment with 10 microg/L TGFbeta1, AP1, SP1 and Smad3-Smad4 were activated and the expression of collagen I and PAI-1 were increased.


CONCLUSIONS
TGFbeta1 may activate SP1, AP1 and Smad3-Smad4 and thus increase the expression of PAI-1 and collagen I.
Li
Yang
Y
Beijing Institute of Radiation Medicine, Beijing 100850, China. leeyoung33@vip.sina.com
Wang
De-wen
DW
Song
Liang-wen
LW
Peng
Rui-yun
RY
Gao
Ya-bing
YB
Ma
Jun-jie
JJ
D000818:Animals; D002478:Cells, Cultured; D024202:Electrophoretic Mobility Shift Assay; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology*; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar; D051900:Smad3 Protein / Q000235:genetics*; D051901:Smad4 Protein / Q000235:genetics*; D016329:Sp1 Transcription Factor / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D051900:Smad3 Protein; C497787:Smad3 protein, rat; D051901:Smad4 Protein; C490811:Smad4 protein, rat; D016329:Sp1 Transcription Factor; D016212:Transforming Growth Factor beta