title: ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane.
abstract: Ribosomes translating secretory and membrane proteins are targeted to the endoplasmic reticulum membrane and attach to the protein-conducting channel and ribosome-associated membrane proteins (RAMPs). Recently, a new RAMP, ERj1p, has been identified that recruits BiP to ribosomes and regulates translational activity. Here we present the cryo-EM structure of a ribosome-ERj1p complex, revealing how ERj1p coordinates the ribosome at the membrane and how allosteric effects may mediate ERj1p's regulatory activity.
authors.affiliation: Institut für Biochemie der Charité, University Medical School Berlin, Monbijoustr. 2, 10117 Berlin, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Blau
authors.forename: Srinivas
authors.initials: S
authors.lastname: Mullapudi
authors.forename: Thomas
authors.initials: T
authors.lastname: Becker
authors.forename: Johanna
authors.initials: J
authors.lastname: Dudek
authors.forename: Richard
authors.initials: R
authors.lastname: Zimmermann
authors.forename: Pawel A
authors.initials: PA
authors.lastname: Penczek
authors.forename: Roland
authors.initials: R
authors.lastname: Beckmann
mesh_terms: D000818:Animals; D020285:Cryoelectron Microscopy; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005786:Gene Expression Regulation / Q000235:genetics*; D050956:HSP40 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D013329:Structure-Activity Relationship
chemical_list: C092319:Dnajc1 protein, mouse; D000091342:Endoplasmic Reticulum Chaperone BiP; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins