Characterisation of glucose transporter (GLUT) gene expression in broiler chickens.
1. Glucose transporter (GLUT) proteins, one of which is the major insulin-responsive transporter GLUT4, play a crucial role in cellular glucose uptake and glucose homeostasis in mammals. The aim of this study was to identify the extent of mRNA expression of GLUT1, GLUT2, GLUT3 and GLUT8 in chickens intrinsically lacking GLUT4. 2. GLUT1 mRNA was detected in most tissues of 3-week-old broiler chickens, with the highest expression measured in brain and adipose tissue. GLUT2 was expressed only in the liver and kidney. GLUT3 was highly expressed in the brain. GLUT8 was expressed ubiquitously, with expression in kidney and adipose tissue relatively higher than that of other tissues. 3. Expression levels of GLUT isoforms 1, 3 and 8 in skeletal muscle tissue were very low compared to the other tissues tested. 4. [3H]Cytochalasin B binding assays on tissue from 3-week-old chickens showed that the number of cytochalasin B binding sites in skeletal muscle plasma membranes was higher than in liver plasma membranes. These results suggest that GLUT proteins and/or GLUT-like proteins that bind cytochalasin B are expressed in chicken skeletal muscles. 5. It is proposed that GLUT expression and glucose transport in chicken tissues are regulated in a manner different from that in mammals.
Kono
T
T
Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Nishida
M
M
Nishiki
Y
Y
Seki
Y
Y
Sato
K
K
Akiba
Y
Y
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D001921:Brain / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D013737:Testis / Q000378:metabolism
D030161:Avian Proteins; D051246:Glucose Transport Proteins, Facilitative; D020033:Protein Isoforms; D012333:RNA, Messenger