Long-QT syndrome-related sodium channel mutations probed by the dynamic action potential clamp technique.
Long-QT3 syndrome (LQT3) is linked to cardiac sodium channel gene (SCN5A) mutations. In this study, we used the 'dynamic action potential clamp' (dAPC) technique to effectively replace the native sodium current (I(Na)) of the Priebe-Beuckelmann human ventricular cell model with wild-type (WT) or mutant I(Na) generated in a human embryonic kidney (HEK)-293 cell that is voltage clamped by the free-running action potential of the ventricular cell. We recorded I(Na) from HEK cells expressing either WT or LQT3-associated Y1795C or A1330P SCN5A at 35 degrees C, and let this current generate and shape the action potential (AP) of subepicardial, mid-myocardial and subendocardial model cells. The HEK cell's endogenous background current was completely removed by a real-time digital subtraction procedure. With WT I(Na), AP duration (APD) was longer than with the original Priebe-Beuckelmann model I(Na), due to a late I(Na) component of approximately 30 pA that could not be revealed with conventional voltage-clamp protocols. With mutant I(Na), this late component was larger ( approximately 100 pA), producing a marked increase in APD ( approximately 70-80 ms at 1 Hz for the subepicardial model cell). The late I(Na) magnitude showed reverse frequency dependence, resulting in a significantly steeper APD-frequency relation in the mutant case. AP prolongation was more pronounced for the mid-myocardial cell type, resulting in increased APD dispersion for each of the mutants. For both mutants, a 2 s pause following rapid (2 Hz) pacing resulted in distorted AP morphology and beat-to-beat fluctuations of I(Na). Our dAPC data directly demonstrate the arrhythmogenic nature of LQT3-associated SCN5A mutations.
Berecki
Géza
G
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, The Netherlands. g.berecki@amc.uva.nl
Zegers
Jan G
JG
Bhuiyan
Zahurul A
ZA
Verkerk
Arie O
AO
Wilders
Ronald
R
van Ginneken
Antoni C G
AC
D000200:Action Potentials; D001145:Arrhythmias, Cardiac / Q000235:genetics / Q000503:physiopathology; D002460:Cell Line; D004594:Electrophysiology; D006352:Heart Ventricles / Q000166:cytology; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000196:embryology / Q000502:physiology; D008133:Long QT Syndrome / Q000235:genetics* / Q000503:physiopathology; D008954:Models, Biological; D009154:Mutation; D062554:NAV1.5 Voltage-Gated Sodium Channel; D018408:Patch-Clamp Techniques; D015222:Sodium Channels / Q000235:genetics* / Q000502:physiology; D013997:Time Factors; D014162:Transfection; D016276:Ventricular Function
D062554:NAV1.5 Voltage-Gated Sodium Channel; C568320:SCN5A protein, human; D015222:Sodium Channels