title: Folded RNA from an intron of one gene might inhibit expression of a counteracting gene.
abstract: Homeostatic maintenance of mRNA levels including prompt availability of mRNAs for translation in response to changing protein demands might be partly enabled by a system of combinatorial controls involving noncoding RNA blocking agents. This article proposes a specific version of that control mechanism, namely, a double-stranded RNA folding from transcription of an intron of one gene might and leading to an agent that inhibits mRNA of a counteracting gene. Thus transcription of the first gene would automatically repress translation of the second. On the basis of a bioinformatics search, we suggest a possible example, namely, that pro-apoptosis gene PAR4 might inhibit anti-apoptosis gene XIAP. Part of an intron from PAR4 folds to form a large, stable hairpin, and reverse complement of the hairpin stem (with approximately 280 nucleotides) matches a sub-sequence of an exon in XIAP. This would be part of an efficient system to drive initiated apoptosis to its conclusion. Figuratively speaking, it replaces two control knobs with one. Since repeats, some with many thousands of copies, occur throughout the genome with complex distributions, care must be taken before asserting that the presence of any repeat in any gene is significant. Our apoptosis example involves repeats, so experimental verification is needed and planned. However, if it is found that the noncoding RNA by-product of one gene folds into a hairpin that is processed into an agent that inhibits a counteracting gene, then the same type of control unit might be found extensively among counteracting families of genes of many types.
authors.affiliation: Department of Psychiatry, University of North Carolina at Chapel Hill, USA. clark_jeffries@med.unc.edu
authors.forename: Clark
authors.initials: C
authors.lastname: Jeffries
authors.forename: Michael
authors.initials: M
authors.lastname: Jarstfer
authors.forename: Diana O
authors.initials: DO
authors.lastname: Perkins
mesh_terms: D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D005786:Gene Expression Regulation; D006706:Homeostasis; D006801:Humans; D007438:Introns; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D018179:Receptors, Thrombin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D018179:Receptors, Thrombin; D051636:X-Linked Inhibitor of Apoptosis Protein; C112908:protease-activated receptor 4