Identification of RecQL1 as a Holliday junction processing enzyme in human cell lines.
Homologous recombination provides an effective way to repair DNA double-strand breaks (DSBs) and is required for genetic recombination. During the process of homologous recombination, a heteroduplex DNA structure, or a 'Holliday junction' (HJ), is formed. The movement, or branch migration, of this junction is necessary for recombination to proceed correctly. In prokaryotes, the RecQ protein or the RuvA/RuvB protein complex can promote ATP-dependent branch migration of Holliday junctions. Much less is known about the processing of Holliday junctions in eukaryotes. Here, we identify RecQL1 as a predominant ATP-dependent, HJ branch migrator present in human nuclear extracts. A reduction in the level of RecQL1 induced by RNA interference in HeLa cells leads to an increase in sister chromatid exchange. We propose that RecQL1 is involved in the processing of Holliday junctions in human cells.
LeRoy
Gary
G
Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544-1014, USA. gleroy@princeton.edu
Carroll
Robert
R
Kyin
Saw
S
Seki
Masayuki
M
Cole
Michael D
MD
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D004265:DNA Helicases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D045566:DNA, Cruciform / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D034622:RNA Interference; D053484:RecQ Helicases; D012854:Sister Chromatid Exchange
D045566:DNA, Cruciform; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C506731:RECQL4 protein, human; D004265:DNA Helicases; D053484:RecQ Helicases