Delivery of human acetylcholinesterase by adeno-associated virus to the acetylcholinesterase knockout mouse.
The purpose of this work was to develop a gene delivery system that expressed acetylcholinesterase (AChE) for prolonged periods. An adeno-associated virus (AAV) expressing human AChE was constructed by co-transfecting three plasmids into HEK 293T cells. The purified vector expressed 0.17 microg AChE per 1 million viral particles in culture medium in 23 h, or 0.8 U/ml. The AAV/hAChE was injected into muscle of adult AChE knockout mice and into the brains of 3-6 week old AChE knockout mice. Intramuscular injection yielded plasma AChE levels approaching 50% of the AChE activity of wild-type mouse plasma. The highest AChE activity was found on day 3 post-injection. AChE activity declined thereafter to a constant 7% of normal. The decreased level was accompanied by the appearance of anti-human AChE antibodies, suggesting partial clearance of AChE from plasma by antibodies. Intrastriatal injection resulted in AChE expression in the striatum. No antibodies were detected in animals treated intrastriatally. Motor coordination was improved and the lifespan of intrastriatally-treated AChE knockout mice was prolonged. Human AChE was expressed in mouse brain for up to 7 months after intrastriatal injection of an AAV/hAChE construct. Gene-therapy to supply AChE to the striatum improved motor coordination and prolonged the life of mice genetically deficient in AChE, probably by reducing their susceptibility to spontaneous seizures. This supports the hypothesis that their seizures are induced by excess acetylcholine.
Hrabovska
Anna
A
Eppley Institute, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198-6805, USA. hrabovska@fpharm.uniba.sk
Duysen
Ellen G
EG
Sanders
Jeffrey D
JD
Murrin
L Charles
LC
Lockridge
Oksana
O
D000110:Acetylcholinesterase / Q000172:deficiency* / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000375:Aging / Q000502:physiology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001921:Brain / Q000201:enzymology; D002460:Cell Line; D000229:Dependovirus / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression; D006801:Humans; D007273:Injections, Intramuscular; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D015996:Survival Rate
D000906:Antibodies; D000110:Acetylcholinesterase