The major Aeromonas veronii outer membrane protein: gene cloning and sequence analysis.
The gene encoding the major outer membrane protein (OMP) from Aeromonas veronii, Omp38, was cloned and characterized. Sequence analysis revealed an open reading frame of 1,047 nucleotides coding for a primary protein of 349 amino acids with a 20-amino-acid signal peptide at the N-terminal and the consensus sequence Ala-X-Ala (Ala-Asn-Ala) as the signal peptidase I recognition site. The mature protein is composed of 329 amino acids with a calculated molecular mass of 36,327 Da. The degree of identity of the deduced Omp38 amino acid sequence to porins from enteric bacteria (OmpF, PhoE, and OmpC) was only 30%. Nevertheless, Omp38 possesses typical features of Gram-negative porins, including acidic pI, high glycine and low proline content, no cysteine residues, and a carboxy-terminal Phe. On the basis of PhoE-OmpF three-dimensional structure and the Kyte-Doolittle hydrophobicity analysis, it seems likely that Omp38 secondary structure consists of 16 antiparallel beta-strands and 8 loops. Phylogenetic analyses among Omp38 and related porins from Gram-negative bacteria originate well-defined clusters that agree with the taxonomy of the corresponding organisms.
Vázquez-Juárez
Roberto Carlos
RC
Departamento de Patología Marina, Centro de Investigaciones Biológicas del Noroeste, Av. Mar Bermejo 195, Col. Playa Palo Santa Rita, P. O. Box 128, La Paz, BCS, México. cvazquez04@cibnor.mx
Gómez-Chiarri
Marta
M
Barrera-Saldaña
Hugo
H
Hernández
Norma
N
Ascencio
Felipe
F
D000333:Aeromonas / Q000235:genetics*; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003545:Cysteine / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D057927:Hydrophobic and Hydrophilic Interactions; D007526:Isoelectric Point; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010649:Phenylalanine / Q000235:genetics; D010802:Phylogeny; D018272:Porins / Q000235:genetics; D011392:Proline / Q000235:genetics; D021382:Protein Sorting Signals; D017433:Protein Structure, Secondary; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D018272:Porins; D021382:Protein Sorting Signals; D010649:Phenylalanine; D011392:Proline; D003545:Cysteine