Synchrotron radiation circular dichroism spectroscopy of ribose and deoxyribose sugars, adenosine, AMP and dAMP nucleotides.
Synchrotron radiation circular dichroism (SRCD) spectra of ribose and deoxyribose sugars, adenosine, AMP and dAMP nucleotides and cyclic derivatives were measured in the vacuum ultraviolet region (down to 168 nm for sugars and 175 nm for adenine derivatives) and at different pH values (3, 6-7, 9-10) and temperatures (between 5 and 45 degrees C). The information content in the VUV region is important since the CD bands strongly depend on the chemical structure of the sugar, the presence and orientation of a phosphate group and the protonation state of adenine. On the other hand, single or double deprotonation of the phosphoric acid group has no influence on the spectra. We assign the vacuum ultraviolet (VUV) CD bands of the nucleoside and nucleotides to be due mainly to n-->pi* transitions in the adenine nucleobase based on a comparison with the absorption spectra. The CD bands of the sugars are due to n(O -->sigma*) transitions and are much smaller than the CD signal from the nucleotides in the VUV region. Bands are assigned to both pyranose and open-chain forms.
Nielsen
Steen Brøndsted
SB
Department of Physics and Astronomy, University of Aarhus, Ny Munkegade, DK-8000 Aarhus C, Denmark. goetz.bucher@ruhr-uni-bochum.de
Chakraborty
Tapas
T
Hoffmann
Søren Vrønning
SV
D000241:Adenosine / Q000737:chemistry*; D000249:Adenosine Monophosphate / Q000737:chemistry*; D002942:Circular Dichroism; D003838:Deoxyadenine Nucleotides / Q000737:chemistry*; D003855:Deoxyribose / Q000737:chemistry*; D015394:Molecular Structure; D012266:Ribose / Q000737:chemistry*; D017356:Synchrotrons
D003838:Deoxyadenine Nucleotides; D000249:Adenosine Monophosphate; D003855:Deoxyribose; C012873:2'-deoxy-5'-adenosine monophosphate; D012266:Ribose; D000241:Adenosine