Osteogenic potential of mesenchymal stem cells from bone marrow in situ: role of physicochemical properties of artificial surfaces.
Correlation analysis demonstrated the role of inorganic parameters of the surfaces of calcium phosphate materials in the regulation of osteogenic differentiation of mesenchymal precursors. The progenitor stromal cells were isolated from syngeneic bone marrow immobilized in vitro on calcium phosphate surfaces with different structure, phasic, and elemental composition. After 45 days of subcutaneous ectopic osteogenesis in BALB/c mice, the tissues grown on these matrixes were characterized histologically. It was found that adhesion of bone marrow cells is the initial stage determining their future proliferation (conduction) over the artificial surface and the area of formed tissue plate. The success of histogenesis depends on surface roughness. The optimal roughness class was 4-5 (Russian State Standards), which enables differentiation of progenitor stromal cells under the specific microenvironmental conditions into the connective and adipose tissue cells. Differentiation of the progenitor cells into the stromal cells producing the hemopoiesis-inducing microenvironment also takes place in the foci of active hemopoiesis. Induction of osteogenic potential of the stromal precursors (osteoinduction) is determined by the ratio between calcium and phosphate atoms in surface coatings. In our experimental system, osteogenic differentiation of stromal mechanocytes was blocked only at Ca/P<0.5.
Khlusov
I A
IA
Siberian State Medical University, Tomsk, Russia. khl@ultranet.tomsk.ru
Karlov
A V
AV
Sharkeev
Yu P
YP
Pichugin
V F
VF
Kolobov
Yu P
YP
Shashkina
G A
GA
Ivanov
M B
MB
Legostaeva
E V
EV
Sukhikh
G T
GT
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002130:Calcium Phosphates / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D009999:Ossification, Heterotopic / Q000378:metabolism / Q000503:physiopathology*; D010012:Osteogenesis / Q000502:physiology*; D018709:Statistics, Nonparametric; D013499:Surface Properties
D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; C020243:calcium phosphate