[The expression and the significance of L-FABP and FATP4 in the development of nonalcoholic fatty liver disease in rats].
OBJECTIVE
To study the effect of liver fatty acid binding protein(L-FABP) and fatty acid transport protein (FATP4) in the development of nonalcoholic fatty liver disease (NAFLD) in rats.


METHODS
The expression of L-FABP and FATP4 genes was examined in fatty liver rats by reverse transcription and polymerase chain reaction amplification and Western blot methods.


RESULTS
In the high fat diet group (F), mRNA and protein expression of L-FABP and FATP4 were increased at 2 weeks, and they increased remarkably at 12 weeks (P < 0.05; L-FABP mRNA F=124.9, protein expression F=92.6; FATP4 mRNA F=602.9, protein expression F=108.8).


CONCLUSIONS
The high expression of L-FABP and FATP4 at the early stage is an adaptive reaction of the body, With the advanced expression of the L-FABP and FATP4, it can lead to a fatty acid disequilibrium and then result in nonalcoholic fatty liver disease in the rats.
Feng
Ai-juan
AJ
Department of Internal Medicine, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Chen
Dong-feng
DF
D000818:Animals; D050612:Fatty Acid Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005234:Fatty Liver / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D050612:Fatty Acid Transport Proteins; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; C493571:SLC27A4 protein, human