Different effects of enhanced and reduced expression of pub gene on the formation of embryoid bodies by cultured embryonic mouse stem cell.
The effects of pub gene on proliferation and initial stages of differentiation of embryonic mouse stem cells were studied in vitro. To this end we used enhanced expression of human pub gene (hpub) and suppression of expression of mouse endogenous pub gene with RNA-interference in embryonic stem cells. Proliferative activity of genetically modified polyclonal lines of the embryonic stem cells transfected with plasmids carrying expressing hpub gene or plasmids generating small interference RNA to this gene did not differ from that of the control cells. Inhibition of expression of endogenous pub gene in embryonic stem cells using small interference RNA 2-fold decreased the formation of embryoid bodies, at the same time additional expression of exogenous hpub gene almost 2-fold increased their number in comparison with the control. It was hypothesized that pub gene participates in early stages of differentiation of embryonic stem cells leading to the formation of embryoid bodies.
Novosadova
E V
EV
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.
Manuilova
E S
ES
Arsen'eva
E L
EL
Khaidarova
N V
NV
Dolotov
O V
OV
Inozemtseva
L S
LS
Kozachenkov
K Yu
KY
Tarantul
V Z
VZ
Grivennikov
I A
IA
D000704:Analysis of Variance; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D017931:DNA Primers; D004622:Embryo, Mammalian / Q000166:cytology*; D005822:Genetic Vectors / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology*; D014162:Transfection; D000070584:Tripartite Motif Proteins
D002352:Carrier Proteins; D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; C481145:TRIM14 protein, human; D000070584:Tripartite Motif Proteins