Isolation and characterization of a satellite DNA family in Achirus lineatus (Teleostei: Pleuronectiformes: Achiridae).
Agarose gels stained with Ethidium bromide and Southern blot experiments of HindIII-digested genomic DNA of Achirus lineatus evidenced the presence of monomers and multimers of a DNA segment of about 200 bp, named here Al-HindIII sequence. No signals were observed in Southern blot experiments with genomic DNA of other flatfish species. The DNA sequencing of four recombinant clones showed that Al-HindIII sequences had 204 bp and were 63.72% AT-rich. FISH experiments using a Al-HindIII sequence as probe showed bright signals in the centromeric position of all chromosomes of A. lineatus.
Carvalho de Azevedo
Marisa Fagundes
MF
Departamento de Morfologia, Instituto de Biociências, UNESP-Universidade Estadual Paulista , 18618-000 Botucatu, SP, Brazil. marisafca@yahoo.com
Oliveira
Claudio
C
Martins
Cesar
C
Pinto Wasko
Adriane
A
Foresti
Fausto
F
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002875:Chromosomes / Q000235:genetics; D004276:DNA, Satellite / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D005412:Flatfishes / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite