title: Identification of mouse AAV capsid-specific CD8+ T cell epitopes.
abstract: Adeno-associated virus has been developed for use as a gene transfer vector. To understand the impact of AAV capsid-specific CD8(+) T cells on AAV-mediated gene transfer, we identified CD8(+) T cell epitopes for AAV-2 and AAV-8 capsid in C57BL/6 (H-2(b) MHC haplotype) and BALB/c (H-2(d) MHC haplotype) mice. Mice of both the H-2(b) and the H-2(d) haplotypes recognized epitopes on AAV-2 and AAV-8 capsid. T cells from H-2(b) mice recognized an epitope that was conserved between AAV-2 and AAV-8 capsid. Cross-reactivity of AAV-specific CD8(+) T cells induced by different AAV serotypes may have important implications for gene transfer. Identification of these epitopes will facilitate studies of immune response to AAV capsid in mouse models.
authors.affiliation: The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
authors.forename: Denise E
authors.initials: DE
authors.lastname: Sabatino
authors.forename: Federico
authors.initials: F
authors.lastname: Mingozzi
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Hui
authors.forename: Haifeng
authors.initials: H
authors.lastname: Chen
authors.forename: Peter
authors.initials: P
authors.lastname: Colosi
authors.forename: Hildegund C J
authors.initials: HC
authors.lastname: Ertl
authors.forename: Katherine A
authors.initials: KA
authors.lastname: High
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D036022:Capsid Proteins / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics*; D000939:Epitopes / Q000737:chemistry; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry*; D018014:Gene Transfer Techniques; D005821:Genetic Techniques; D015316:Genetic Therapy / Q000379:methods*; D006239:Haplotypes; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D013045:Species Specificity
chemical_list: D036022:Capsid Proteins; D016207:Cytokines; D000939:Epitopes; D018984:Epitopes, T-Lymphocyte; D019151:Peptide Library; D010455:Peptides