title: Aberrant termination triggers nonsense-mediated mRNA decay.
abstract: NMD (nonsense-mediated mRNA decay) is a cellular quality-control mechanism in which an otherwise stable mRNA is destabilized by the presence of a premature termination codon. We have defined the set of endogenous NMD substrates, demonstrated that they are available for NMD at every round of translation, and showed that premature termination and normal termination are not equivalent biochemical events. Premature termination is aberrant, and its NMD-stimulating defects can be reversed by the presence of tethered poly(A)-binding protein (Pab1p) or tethered eRF3 (eukaryotic release factor 3) (Sup35p). Thus NMD appears to be triggered by a ribosome's failure to terminate adjacent to a properly configured 3'-UTR (untranslated region), an event that may promote binding of the UPF/NMD factors to stimulate mRNA decapping.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, USA.
authors.forename: N
authors.initials: N
authors.lastname: Amrani
authors.forename: S
authors.initials: S
authors.lastname: Dong
authors.forename: F
authors.initials: F
authors.lastname: He
authors.forename: R
authors.initials: R
authors.lastname: Ganesan
authors.forename: S
authors.initials: S
authors.lastname: Ghosh
authors.forename: S
authors.initials: S
authors.lastname: Kervestin
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: D A
authors.initials: DA
authors.lastname: Mangus
authors.forename: P
authors.initials: P
authors.lastname: Spatrick
authors.forename: A
authors.initials: A
authors.lastname: Jacobson
mesh_terms: D020413:3' Untranslated Regions; D018389:Codon, Nonsense; D010454:Peptide Termination Factors; D039122:Poly(A)-Binding Protein I / Q000378:metabolism; D011328:Prions / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D018389:Codon, Nonsense; D010454:Peptide Termination Factors; D039122:Poly(A)-Binding Protein I; D011328:Prions; D012333:RNA, Messenger; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins