title: Use of reference tissue models for quantification of histamine H1 receptors in human brain by using positron emission tomography and [11c]doxepin.
abstract: The aim of the present study is to evaluate the validity of the simplified reference tissue model (SRTM) and of Logan graphical analysis with reference tissue (LGAR) for quantification of histamine H1 receptors (H1Rs) by using positron emission tomography (PET) with [11C]doxepin. These model-based analytic methods (SRTM and LGAR) are compared to Logan graphical analysis (LGA) and to the one-tissue model (1TM), using complete datasets obtained from 5 healthy volunteers. Since HIR concentration in the cerebellum can be regarded as negligibly small, the cerebellum was selected as the reference tissue in the present study. The comparison of binding potential (BP) values estimated by LGAR and 1TM showed good agreement; on the other hand, SRTM turned out to be unstable concerning parameter estimation in several regions of the brain. By including the results of noise analysis, LGAR became a reliable method for parameter estimation of [11C]doxepin data in the cortical regions.
authors.affiliation: Department of Quantum Science and Energy Engineering, Tohoku University, Sendai, Japan.
authors.forename: Atsuro
authors.initials: A
authors.lastname: Suzuki
authors.forename: Manabu
authors.initials: M
authors.lastname: Tashiro
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kimura
authors.forename: Hideki
authors.initials: H
authors.lastname: Mochizuki
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishii
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Watabe
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yanai
authors.forename: Kiichi
authors.initials: K
authors.lastname: Ishiwata
authors.forename: Keizo
authors.initials: K
authors.lastname: Ishii
mesh_terms: D000328:Adult; D000465:Algorithms; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism*; D001931:Brain Mapping / Q000379:methods; D002250:Carbon Radioisotopes; D003198:Computer Simulation; D004316:Doxepin; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods* / Q000592:standards; D008297:Male; D008959:Models, Neurological; D019047:Phantoms, Imaging; D049268:Positron-Emission Tomography / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D019275:Radiopharmaceuticals; D011969:Receptors, Histamine H1 / Q000378:metabolism*; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002250:Carbon Radioisotopes; D019275:Radiopharmaceuticals; D011969:Receptors, Histamine H1; D004316:Doxepin