title: C-21 steroidal glycosides from the roots of Cynanchum chekiangense and their immunosuppressive activities.
abstract: Two new C21 steroidal glycosides, chekiangensosides A and B, were isolated from the roots of Cynanchum chekiangense, together with two known compounds. On the basis of chemical evidence and extensive spectroscopic methods, including one-dimensional and two-dimensional NMR, the structures of two new compounds were identified as cynajapogenin A, 3-O-beta-D-glucopyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-alpha-L-cymaropyranosyl-(1-->4)-beta-D-cymaropyranoside, and glaucogenin A, 3-O-beta-D-glucopyranosyl-(1-->4)-beta-D-cymaropyranosyl-(1-->4)-alpha-L-cymaropyranosyl-(1-->4)-beta-D-cymaropyranoside, respectively. The two known steroidal glycosides, and were revised. These isolated compounds were tested for their immunological activities in vitro against concanavalin A (Con A)- and lipopolysaccharide (LPS)-induced proliferation of mice splenocytes. Compounds showed immunosuppressive activities in vitro in a dose-dependent manner.
authors.affiliation: Department of Chemistry, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Sun
authors.forename: Yiping
authors.initials: Y
authors.lastname: Ye
authors.forename: Fengyang
authors.initials: F
authors.lastname: Chen
authors.forename: Yuanjiang
authors.initials: Y
authors.lastname: Pan
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006027:Glycosides / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging; D051379:Mice; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry*; D013154:Spleen / Q000166:cytology; D013256:Steroids / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D007166:Immunosuppressive Agents; D012503:Saponins; D013256:Steroids; C509627:cynajapogenin A 3-O-glucopyanosyl-1-4-cymaropyranosyl-1-4-cymaropyranosyl-1-4-cymaropyranoside; C509628:glaucogenin A 3-O-glucopyranosyl-1-4-cymaropyranosyl-1-4-cymaropyranosyl-1-4-cymaropyranoside