Effects of hepatitis B virus on p53 expression in hepatoma cell line SMMU-7721.
OBJECTIVE
To investigate the contribution of HBV in the development of hepatocarcinoma by examining the effects of HBV on p53 function in SMMU-7721 cell line.


METHODS
Plasmid pCMVp53 was transfected or cotransfected with pCMVHBVa (wild-type HBV) or PCMVHBVb (mutation type HBV) into the hepatoma cell line SMMU-7721 by lipofectamine. Apoptosis cells were labeled with annexin V-FITC and confirmed by flow cytometry. Reporter plasmid PG13-CAT or p21-luc was cotransfected, respectively, into each group to determine the transactivation activity of p53 and its effect on p21 promoter. Western blot was performed to observe p53 expression in hepatoma cell line of each group.


RESULTS
The group transfected with pCMVp53 alone exhibited higher luciferase activity and higher apoptosis rate, otherwise, the p53 expression and reporter activity of PG13-CAT or P21-luc as well as cell apoptosis rate were obviously higher in the group cotransfected of pCMVp53 with pCMVHBVa, but not in the other cotransfected group.


CONCLUSIONS
Transient transfection of HBV into the SMMU-7721 cell line can enhance p53 expression and its effects on development of hepatocarcinoma.
Qu
Jian-Hui
JH
Department of Pathology, the Second Military Medical University, Shanghai 200433, China. mhzhu2000@hotmail.com
Zhu
Ming-Hua
MH
Lin
Jing
J
Ni
Can-Rong
CR
Li
Fang-Mei
FM
Zhu
Zhi
Z
Yu
Guan-Zhen
GZ
D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D002455:Cell Division; D045744:Cell Line, Tumor; D006509:Hepatitis B / Q000150:complications* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D016159:Tumor Suppressor Protein p53