Evidence for horizontal transfer of 1-aminocyclopropane-1-carboxylate deaminase genes.
PCR was used to rapidly identify and isolate 1-aminocyclopropane-1-carboxylate (ACC) deaminase genes from bacteria. The Shimodaira-Hasegawa test was used to assess whether phylogenetically anomalous gene placements suggestive of horizontal gene transfer (HGT) were significantly favored over vertical transmission. The best maximum likelihood (ML) ACC deaminase tree was significantly more likely than four alternative ML trees, suggesting HGT.
Hontzeas
N
N
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA. nhontzea@purdue.edu
Richardson
A O
AO
Belimov
A
A
Safronova
V
V
Abu-Omar
M M
MM
Glick
B R
BR
D019755:Carbon-Carbon Lyases / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis; D022761:Gene Transfer, Horizontal; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases