Production of a monoclonal antibody against ochratoxin A and its application to immunochromatographic assay.
A monoclonal antibody (Mab) against ochratoxin A (OTA) was produced from the hybridoma cell line C7G25, which was established by the fusion of Sp2/0-Ag14 myeloma cells with spleen cells isolated from a BALB/c mouse immunized with the OTA-bovine serum albumin conjugate. This Mab belongs to the IgG(2a) heavy-chain subclass with a kappa-type light chain. The level of 50% inhibition concentration was 1.20 ng/mL in a competitive direct enzyme-linked immunosorbent assay (cdELISA), and the detection limit was 0.12 ng/mL. This antibody is specific for OTA but also shows cross-reactivity with ochratoxin B (31.7%) in a cdELISA. On the basis of the sandwich format using the produced Mab against OTA, a rapid immunochromatographic assay was developed to efficiently detect OTA. This method was able to detect up to 500 ng/mL of OTA in <10 min.
Cho
Young-Jin
YJ
School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, Korea.
Lee
Dae-Hee
DH
Kim
Dae-Ok
DO
Min
Won-Ki
WK
Bong
Ki-Tae
KT
Lee
Gang-Gweon
GG
Seo
Jin-Ho
JH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D002845:Chromatography / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D017979:Gold Colloid; D006825:Hybridomas / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D009793:Ochratoxins / Q000032:analysis* / Q000276:immunology*; D011786:Quality Control
D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D009793:Ochratoxins; C025589:ochratoxin A