[The biotransformation of kaempferitrin by human intestinal flora].
OBJECTIVE
To study the biotransformation of kaempferitrin, a major chemical principle of the fruits of Siraitia grosvenori (Swingle) C. Jeffery, with human intestinal flora.


METHODS
The kaempferitrin was incubated with human intestinal flora. The biotransformation products were isolated and purified by chromatographic methods and the structures were determined by spectroscopic techniques.


RESULTS
Kaempferitrin was converted into kaempferol 3-O-alpha-L-rhamnoside (afzelin, I) , kaempferol 7-O-alpha-L-rhamnoside (II), kaempferol (III) and p-hydroxybenzoic acid (IV) by human intestinal flora. rhamnoside (II), kaempferol (III) and p-hydroxybenzoic acid (IV) by human intestinal flora.


CONCLUSIONS
The structure of kaempferitrin can be biotransformatedly converted by human intestinal flora.
Yang
Xiu-wei
XW
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China. xwyang@bjmu.edu.cn
Zhang
Jian-ye
JY
Xu
Wei
W
Li
Jun
J
Zhang
Wei-qing
WQ
D001419:Bacteria / Q000378:metabolism*; D001711:Biotransformation; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D044949:Kaempferols / Q000302:isolation & purification / Q000378:metabolism / Q000493:pharmacokinetics*; D008362:Mannosides / Q000378:metabolism; D029775:Momordica / Q000737:chemistry; D010226:Parabens / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D044945:Proanthocyanidins / Q000378:metabolism
D044949:Kaempferols; D008362:Mannosides; D010226:Parabens; D044945:Proanthocyanidins; C477954:afzelin; C006552:kaempferol; C038193:4-hydroxybenzoic acid; C042728:lespenefril