title: Apolipoprotein A-I induces IL-10 and PGE2 production in human monocytes and inhibits dendritic cell differentiation and maturation.
abstract: Apolipoprotein A-I (apoA-I), the major protein component of serum high-density lipoprotein, exhibits anti-inflammatory activity in atherosclerosis. In this study, we demonstrate that apoA-I inhibits DC differentiation and maturation. DC differentiated from monocytes in the presence of apoA-I showed a decreased expression of surface molecules such as CD1a, CD80, CD86, and HLA-DR. In addition, these DC exhibited decreased endocytic activity and weakened allogeneic T-cell activation. During DC differentiation in the presence of apoA-I, PGE(2) and IL-10, which are known to be DC differentiation inhibitors and/or modulators of DC function, were produced at remarkable rates, whereas IL-12 production in the cells after stimulation with CD40 mAb and IFN-gamma was significantly decreased in comparison with the control DC. T cells stimulated by apoA-I-pretreated DC produced significantly low levels of IFN-gamma, and apoA-I inhibited cross-talk between DC and NK cells, in terms of IL-12 and IFN-gamma production. Therefore, apoA-I appears to play an important role in modulating both innate immune response and inflammatory response. The novel inhibitory function of apoA-I on DC differentiation and function may facilitate the development of new therapeutic interventions in inflammatory diseases.
authors.affiliation: Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-600, Republic of Korea.
authors.forename: Kwang Dong
authors.initials: KD
authors.lastname: Kim
authors.forename: Ho Yong
authors.initials: HY
authors.lastname: Lim
authors.forename: Hee Gu
authors.initials: HG
authors.lastname: Lee
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Yoon
authors.forename: Yong-Kyung
authors.initials: YK
authors.lastname: Choe
authors.forename: Inpyo
authors.initials: I
authors.lastname: Choi
authors.forename: Sang-Gi
authors.initials: SG
authors.lastname: Paik
authors.forename: Young-Sang
authors.initials: YS
authors.lastname: Kim
authors.forename: Young
authors.initials: Y
authors.lastname: Yang
authors.forename: Jong-Seok
authors.initials: JS
authors.lastname: Lim
mesh_terms: D016632:Apolipoprotein A-I / Q000008:administration & dosage*; D002454:Cell Differentiation / Q000187:drug effects; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
chemical_list: D016632:Apolipoprotein A-I; D016753:Interleukin-10; D015232:Dinoprostone