[The relationship between TNF-alpha and the injury of renal tubular cells caused by anoxia/reoxygenation].
OBJECTIVE
To investigate the relationship between TNF-alpha and renal tubular cell injury caused by anoxia/reoxygenation.


METHODS
Human renal proximal tubular cell line HK-2 was used as model. Anoxia/reoxygenation were produced by covering/de-covering the cell culture with liquid paraffin wax. The level of TNF-alpha and the activity of lactate dehydrogenase(LDH) in the culture medium was determined by radioimmunoassay(RIA) and biochemical methods, respectively. Trypan blue exclusion was used to measure cell viability.


RESULTS
Anoxia/reoxygenation could increase TNF-alpha level and LDH activity, but decrease viability of HK-2 cells. TNF-alpha level was positively correlated with LDH activity (r=0.89, P<0.05) and negatively with the cell viability (r=-0.91, P<0.05).


CONCLUSIONS
TNF-alpha induced by anoxia/reoxygenation may participate in the process of renal tubular cell injury.
Dong
Wen-bin
WB
Department of Newborn Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China. dongwenbin2000@163.com
Du
Yi-ting
YT
Chen
Yue
Y
Hang
Yong-lun
YL
Chen
Feng
F
Deng
Cun-liang
CL
D015687:Cell Hypoxia / Q000502:physiology*; D002460:Cell Line; D002470:Cell Survival; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000166:cytology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D011863:Radioimmunoassay; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D014409:Tumor Necrosis Factor-alpha; D007770:L-Lactate Dehydrogenase