title: Oxidative status in patients submitted to conization and radiation treatments for uterine cervix neoplasia.
abstract: BACKGROUND
  Cervical cancer is a major cause of morbidity among women. We investigated the treatment effect on oxidative status from patients submitted to radiotherapy or conization surgery to high-grade SIL (squamous intraepithelial lesion) treatment, and oxidative profile from patients newly diagnosed for uterine cervix cancer, without treatment.
  
  
  METHODS
  We determined the catalase activity in blood, reduced glutathione (GSH) in plasma, TBARS and protein carbonyl content from serum samples of the patients.
  
  
  RESULTS
  The catalase activity, GSH levels, TBARS and protein carbonyl content had no statistical differences related to the controls, neither when the 2 treatments were compared, possibly because the antioxidant defense may be acting in the first period of the neoplasic transformation, and maybe indicating a possible arrest of the tumor cells caused by the efficiency of the treatments. In the non-treated patients, TBARS and protein carbonyl contents, GSH levels and catalase activity were shown to be increased comparing with the treated patients and compared with the controls indicating an tumor effect on oxidative profile, and the antioxidant activity been increased in the beginning of the tumor development.
  
  
  CONCLUSIONS
  We suggest that the treatments were efficient in arrest of the tumor.
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Paula Acosta
authors.initials: PA
authors.lastname: Maldonado
authors.forename: Luiz Augusto
authors.initials: LA
authors.lastname: Negrini
authors.forename: Rosilene Rodrigues
authors.initials: RR
authors.lastname: Kaizer
authors.forename: Rafael Fernandes
authors.initials: RF
authors.lastname: Zanin
authors.forename: Maria do Carmo
authors.initials: Mdo C
authors.lastname: Araújo
authors.forename: Vanessa
authors.initials: V
authors.lastname: Battisti
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000532:radiotherapy / Q000601:surgery; D002374:Catalase / Q000097:blood; D019092:Conization; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation / Q000528:radiation effects; D008875:Middle Aged; D010084:Oxidation-Reduction / Q000528:radiation effects; D050050:Protein Carbonylation / Q000528:radiation effects; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000532:radiotherapy / Q000601:surgery; D002578:Uterine Cervical Dysplasia / Q000097:blood* / Q000532:radiotherapy / Q000601:surgery
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005978:Glutathione