title: Hematopoietic differentiation of embryoid bodies derived from the human embryonic stem cell line SNUhES3 in co-culture with human bone marrow stromal cells.
abstract: Human embryonic stem (ES) cells can be induced to differentiate into hematopoietic precursor cells via two methods: the formation of embryoid bodies (EBs) and co-culture with mouse bone marrow (BM) stromal cells. In this study, the above two methods have been combined by co-culture of human ES-cell-derived EBs with human BM stromal cells. The efficacy of this method was compared with that using EB formation alone. The undifferentiated human ES cell line SNUhES3 was allowed to form EBs for two days, then EBs were induced to differentiate in the presence of a different serum concentration (EB and EB/high FBS group), or co- cultured with human BM stromal cells (EB/BM co-culture group). Flow cytometry and hematopoietic colony-forming assays were used to assess hematopoietic differentiation in the three groups. While no significant increase of CD34+/CD45- or CD34+/CD38- cells was noted in the three groups on days 3 and 5, the percentage of CD34+/CD45- cells and CD34+/ CD38- cells was significantly higher in the EB/BM co-culture group than in the EB and EB/high FBS groups on day 10. The number of colony-forming cells (CFCs) was increased in the EB/BM co-culture group on days 7 and 10, implying a possible role for human BM stromal cells in supporting hematopoietic differentiation from human ES cell-derived EBs. These results demonstrate that co-culture of human ES-cell-derived EBs with human BM stromal cells might lead to more efficient hematopoietic differentiation from human ES cells cultured alone. Further study is warranted to evaluate the underlying mechanism.
authors.affiliation: Division of Hematology/Oncology, Department of Internal Medicine, Korea University Medical Center, 126-1 Anam-dong 5ga, Sungbuk-gu, Seoul 136-705, Korea.
authors.forename: Seok Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Byung Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Suck Won
authors.initials: SW
authors.lastname: Ryu
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Yoo
authors.forename: Jee Hyun
authors.initials: JH
authors.lastname: Oh
authors.forename: Chang Hee
authors.initials: CH
authors.lastname: Song
authors.forename: Sun Haeng
authors.initials: SH
authors.lastname: Kim
authors.forename: Dong Seop
authors.initials: DS
authors.lastname: Choi
authors.forename: Jae Hong
authors.initials: JH
authors.lastname: Seo
authors.forename: Chul Won
authors.initials: CW
authors.lastname: Choi
authors.forename: Sang Won
authors.initials: SW
authors.lastname: Shin
authors.forename: Yeul Hong
authors.initials: YH
authors.lastname: Kim
authors.forename: Jun Suk
authors.initials: JS
authors.lastname: Kim
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D017493:Leukocyte Common Antigens / Q000032:analysis; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000502:physiology
chemical_list: D018952:Antigens, CD34; D017493:Leukocyte Common Antigens; D051997:ADP-ribosyl Cyclase 1