mmpL7 gene of Mycobacterium tuberculosis is responsible for isoniazid efflux in Mycobacterium smegmatis.
The Mycobacterium tuberculosis mmpL7 gene, encoding a hypothetical resistance nodulation division transporter, confers a high resistance level to isoniazid when overexpressed in Mycobacterium smegmatis. The resistance level decreased in the presence of the efflux pump inhibitors reserpine and CCCP (carbonyl cyanide m-chlorophenylhydrazone). Energy-dependent efflux of isoniazid from M. smegmatis cells expressing the mmpL7 gene was observed.
Pasca
Maria R
MR
Dipartimento di Genetica e Microbiologia, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy.
Guglierame
Paola
P
De Rossi
Edda
E
Zara
Francesca
F
Riccardi
Giovanna
G
D000995:Antitubercular Agents / Q000493:pharmacokinetics*; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial / Q000502:physiology*; D007538:Isoniazid / Q000493:pharmacokinetics*; D008826:Microbial Sensitivity Tests; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000235:genetics*
D000995:Antitubercular Agents; D007538:Isoniazid