title: Surface-enhanced Raman scattering detection of lysophosphatidic acid.
abstract: Surface-enhanced Raman scattering using silver nanoparticles was applied to detect various forms of lysophosphatidic acid (LPA) to examine its potential application as an alternative to current detection methods of LPA as biomarkers of ovarian cancer. Enhancement of the Raman modes of the molecule, especially those related to the acyl chain within the 800-1300 cm(-1) region, was observed. In particular, the C-C vibration mode of the gauche-bonded chain around 1100 cm(-1) was enhanced to allow the discrimination of two similar LPA molecules. Given the molecular selectivity of this technique, the detection of LPA using SERS may eliminate the need for partial purification of samples prior to analysis in cancer screening.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA.
authors.forename: Leo
authors.initials: L
authors.lastname: Seballos
authors.forename: Jin Z
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Rebecca
authors.initials: R
authors.lastname: Sutphen
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry*; D022081:Cell Surface Extensions; D005260:Female; D006801:Humans; D008246:Lysophospholipids / Q000032:analysis* / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D008246:Lysophospholipids; D012834:Silver; C032881:lysophosphatidic acid