Lansoprazole binding to the neutrophils in dextran sulfate sodium-induced rat colitis.
To clarify the effector sites of lansoprazole in the colonic mucosa during the formation of colitis, dextran sulfate sodium-induced colitis was induced by the oral administration of 3% aqueous solution for 3 and 7 days. The effector sites of [3H]lansoprazole were examined by the intra-aortic infusion of the radiolabelled compound and the autoradiographic tracing of water-soluble compounds. As a result, the [3H]lansoprazole binding in the control rat colon was negligible, while in dextran sulfate sodium-treated rats specific binding sites of [3H]lansoprazole were recognized in the cytoplasm of the mesenchymal cells, and most of them coincided with polymorphonuclear leucocytes and macrophages.
Nakamura
Masahiko
M
Center for Clinical Pharmacy and Clinical Sciences, School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan. nakamuram@pharm.kitasato-u.ac.jp
Asada
Maki
M
Atsuda
Koichiro
K
Matsui
Hidenori
H
Watanabe
Toshio
T
Higuchi
Kazuhide
K
Arakawa
Tetsuo
T
Hibi
Norifumi
N
Tsuchimoto
Kanji
K
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001345:Autoradiography / Q000379:methods; D001665:Binding Sites; D001667:Binding, Competitive; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism*; D003106:Colon / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D005748:Gastric Fundus / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D007261:Infusions, Intra-Arterial; D007413:Intestinal Mucosa / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D064747:Lansoprazole; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D009853:Omeprazole / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D054328:Proton Pump Inhibitors; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000890:Anti-Infective Agents; D054328:Proton Pump Inhibitors; D064747:Lansoprazole; D014316:Tritium; D016264:Dextran Sulfate; D009853:Omeprazole