3'-modified oligo (2'-O-methylribonucleotides) as improved probes for hybridization with RNA.
A series of octa (2-O-methylribonucleotides) with an additional 3'-terminal deoxynucleoside (T, dC, dA or dG) linked by the 3'-3' (inverted) bond was synthesized. The exceptional stability of these oligomers to a 3'-exonuclease (SVP) and nucleases in culture medium containing 10% heat-inactivated fetal calf serum was demonstrated. It was shown that the addition of the 3'-dangling inverted deoxynucleoside increases substantially the thermal stability of the duplexes of oligo(2'-O-methylribonucleotides) with complementary RNA and DNA in the case of a relatively weak terminal AmU(T) pair and enhances the mismatch sensitivity.
Novopashina
D S
DS
Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia. danov@niboch.nsc.ru
Totskaya
O S
OS
Lomzov
A A
AA
Venyaminova
A G
AG
D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D009843:Oligoribonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D018075:RNA, Complementary / Q000737:chemistry; D012265:Ribonucleotides / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
D018076:DNA, Complementary; D003854:Deoxyribonucleotides; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D018075:RNA, Complementary; D012265:Ribonucleotides; D012313:RNA; D004247:DNA