title: Symbiotic hydrogenase activity in Bradyrhizobium sp. (Vigna) increases nitrogen content in Vigna unguiculata plants.
abstract: Bradyrhizobium sp. (Lupinus) and Bradyrhizobium sp. (Vigna) mutants in which hydrogenase (hup) activity was affected were constructed and analyzed. Vigna unguiculata plants inoculated with the Bradyrhizobium sp. (Vigna) hup mutant showed reduced nitrogenase activity and also a significant decrease in nitrogen content, suggesting a relevant contribution of hydrogenase activity to plant yield.
authors.affiliation: Laboratory of Microbiology, Department of Biotechnology, E.T.S. Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Baginsky
authors.forename: Belén
authors.initials: B
authors.lastname: Brito
authors.forename: Juan
authors.initials: J
authors.lastname: Imperial
authors.forename: Tomás
authors.initials: T
authors.lastname: Ruiz-Argüeso
authors.forename: Jose M
authors.initials: JM
authors.lastname: Palacios
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D020369:Bradyrhizobium / Q000201:enzymology* / Q000235:genetics; D007887:Fabaceae / Q000378:metabolism / Q000382:microbiology*; D005786:Gene Expression Regulation; D006864:Hydrogenase / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism*; D009586:Nitrogen Fixation; D013559:Symbiosis; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; D006864:Hydrogenase; D009584:Nitrogen