title: Genetic alterations and expression of inhibitor of growth 1 in human sporadic colorectal cancer.
abstract: OBJECTIVE
  To explore the effect and significance of inhibitor of growth 1 (ING1) gene in carcinogenesis and progression of human sporadic colorectal cancer.
  
  
  METHODS
  mRNA expression, mutation, and loss of heterozygosity (LOH) of ING1 gene in 35 specimens of sporadic colorectal cancer tissues and the matched normal mucous membrane tissues were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), PCR-single strain conformation polymorphism (PCR-SSCP) and PCR-simple sequence length polymorphism (PCR-SSLP) using microsatellite markers, respectively.
  
  
  RESULTS
  The average ratios of light intensities of p33(ING1b) and p47(ING1a) mRNA expression in the cancerous tissues were significantly lower than those in normal tissues. The difference between the two mRNA splices was not significant in the matched tissues. In addition, the ratios of light intensities of p33(ING1b) and p47(ING1a) mRNA expression in the cancerous tissues of Dukes' stages C and D were significantly lower than those in cancerous tissues of Dukes' stages A and B. However, no mutation of ING1 gene was detected in all 35 cases; only 4 cases of LOH (11.4%) were found.
  
  
  CONCLUSIONS
  p33(ING1b) and p47(ING1a) mRNA expressions are closely related with the carcinogenesis and progression of human sporadic colorectal cancer. No mutation of ING1 gene is found, and there are only few LOH in sporadic colorectal cancers. These might not be the main reasons for the down regulation of ING1 expression. Its low expression may happen in transcription or post-transcription.
authors.affiliation: Department of Coloproctological Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Province, China. clisheng@vip.sina.com
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Chen
authors.forename: Jian-Bao
authors.initials: JB
authors.lastname: Wei
authors.forename: Yong-Chun
authors.initials: YC
authors.lastname: Zhou
authors.forename: Sen
authors.initials: S
authors.lastname: Zhang
authors.forename: Jun-Lin
authors.initials: JL
authors.lastname: Liang
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Cao
authors.forename: Zong-Jiang
authors.initials: ZJ
authors.lastname: Tang
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D019656:Loss of Heterozygosity; D009687:Nuclear Proteins / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: C501503:ING1 protein, human; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D025521:Tumor Suppressor Proteins