title: Functional coupling between vanillate-O-demethylase and formaldehyde detoxification pathway.
abstract: Pseudomonas putida vanillate-O-demethylase consisting of VanA and VanB was expressed in Escherichia coli strain K-12. Recombinant E. coli strain K-12 cells expressing VanAB efficiently converted vanillate into protocatechuate with glucose consumption. Mutant lacking either pgi or zwf showed higher or lower converting activity than the parental strain, respectively. Formaldehyde, which is the by-product of the demethylation, was converted into formate in the cellular reaction. Formate accumulation was blocked by gene disruption of the E. coli frmA that coded glutathione-dependent formaldehyde dehydrogenase.
authors.affiliation: Biofrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machidashi, Tokyo 194-8533, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hibi
authors.forename: Tomonori
authors.initials: T
authors.lastname: Sonoki
authors.forename: Hideo
authors.initials: H
authors.lastname: Mori
mesh_terms: D004274:DNA, Recombinant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005557:Formaldehyde / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000378:metabolism; D008745:Methylation; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010090:Oxidoreductases, O-Demethylating / Q000235:genetics / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics; D014641:Vanillic Acid / Q000378:metabolism
chemical_list: D004274:DNA, Recombinant; D062385:Hydroxybenzoates; D009243:NAD; D005557:Formaldehyde; C009091:protocatechuic acid; D009249:NADP; D010090:Oxidoreductases, O-Demethylating; C023284:vanillate O-demethylase; D014641:Vanillic Acid