title: Molecular identification of Rhizomucor pusillus as a cause of sinus-orbital zygomycosis in a patient with acute myelogenous leukemia.
abstract: Sinus-orbital zygomycosis caused by Rhizomucor pusillus in a patient with acute myelogenous leukemia is described. Identification was achieved by sequencing of the internal transcribed spacer (ITS) regions of the rRNA gene and by expression of zygospores in mating. This report highlights the value of ITS sequencing as a diagnostic tool for the identification of R. pusillus and expands the understanding of infection types caused by this zygomycete.
authors.affiliation: Department of Pathology and Microbiology, University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, NE 68198-6495, USA. piwen@unmc.edu
authors.forename: Peter C
authors.initials: PC
authors.lastname: Iwen
authors.forename: Alison G
authors.initials: AG
authors.lastname: Freifeld
authors.forename: Lynne
authors.initials: L
authors.lastname: Sigler
authors.forename: Stefano R
authors.initials: SR
authors.lastname: Tarantolo
mesh_terms: D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D015522:Frontal Sinusitis / Q000150:complications / Q000175:diagnosis*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000150:complications*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009091:Mucormycosis / Q000150:complications / Q000175:diagnosis*; D009916:Orbital Diseases / Q000150:complications / Q000175:diagnosis*; D020103:Rhizomucor / Q000235:genetics / Q000302:isolation & purification*; D013172:Spores, Fungal / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer