title: Stg 1 is a novel SM22/transgelin-like actin-modulating protein in fission yeast.
abstract: We identified a novel actin-modulating protein Stg 1 in the fission yeast Schizosaccharomyces pombe. Stg 1 is similar to mammalian SM22/transgelin, and biochemical experiments showed that Stg 1 crosslinked F-actin. Microscopic observation suggested that Stg 1 was a component of actin patch. Overexpression of Stg 1 caused a defect in cytokinesis by suppressing the formation of a contractile ring and formation of abnormal aggregates of F-actin in the ends and mid-region of cells. Although distribution of the actin cytoskeleton was not affected by disrupting Stg 1(+), genetic interaction suggested that Stg 1 was likely involved in controlling the organization of the actin cytoskeleton in cell morphogenesis and cytokinesis in fission yeast.
authors.affiliation: Doctoral Program in Structural Biosciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennohdai, Tsukuba, Ibaraki 305-8577, Japan. knakano@biol.tsukuba.ac.jp
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nakano
authors.forename: Fumihide
authors.initials: F
authors.lastname: Bunai
authors.forename: Osamu
authors.initials: O
authors.lastname: Numata
mesh_terms: D000199:Actins / Q000378:metabolism* / Q000648:ultrastructure; D000595:Amino Acid Sequence; D048749:Cytokinesis / Q000235:genetics; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D008840:Microfilament Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D012568:Schizosaccharomyces / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D000199:Actins; D008840:Microfilament Proteins; D029702:Schizosaccharomyces pombe Proteins; C507075:Stg1 protein, S pombe