title: Inhibitory activity of glass-ionomer cements on cariogenic bacteria.
abstract: This study evaluated the antibacterial activity of the glass-ionomer cements Vitrebond (3M ESPE), Ketac Molar (3M ESPE) and Fuji IX (GC America) against S mutans, S sobrinus, L acidophilus and A viscosus, using the agar diffusion test. Inocula were obtained by the seed of indicators cultures in BHI broth incubated at 37 degrees C for 24 hours. Base layers containing 15 mL of BHI agar and 300 microL of each bacteria suspension were prepared in Petri dishes. Six wells measuring 4 mm in diameter were made in each plate and completely filled with one of the testing materials. A 0.2% chlorhexidine solution applied in round filter papers was used as control. Tests were performed 12 times for each material and bacteria strain. After incubation of the plates at 37 degrees C for 24 hours, the zones of bacterial growth inhibition around the wells were measured. Overall, the results showed the following sequence of antibacterial activity: Vitrebond (despite the activation mode) > 0.2% chlorhexidine > Ketac Molar > Fuji IX, according to Kruskal-Wallis and Mann-Whitney statistical tests. This study confirmed significant antibacterial activity for two conventional glass-ionomers and one resin-modified glass-ionomer material. The resin-modified glass-ionomer cement Vitrebond, regardless of the activation mode, presented the best antibacterial activity against S mutans and S sobrinus. The antibacterial activity against A viscosus for Vitrebond was similar to 0.2% chlorhexidine, while light activation reduced its antibacterial activity against L acidophilus.
authors.affiliation: Pediatric Dentistry, University of São Paulo State, School of Dentistry, Araraquara, SP, Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: Duque
authors.forename: Thaís de Cássia
authors.initials: Tde C
authors.lastname: Negrini
authors.forename: Josimeri
authors.initials: J
authors.lastname: Hebling
authors.forename: Denise Madalena Palomari
authors.initials: DM
authors.lastname: Spolidorio
mesh_terms: D016924:Actinomyces viscosus / Q000187:drug effects; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D003731:Dental Caries / Q000382:microbiology*; D005899:Glass Ionomer Cements / Q000737:chemistry / Q000494:pharmacology*; D007779:Lactobacillus acidophilus / Q000187:drug effects; D008689:Methacrylates; D050296:Microbial Viability / Q000187:drug effects*; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000187:drug effects; D016986:Streptococcus sobrinus / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D005899:Glass Ionomer Cements; D008689:Methacrylates; C110713:fuji IX; C110712:ketac-molar; C067790:Vitrabond; C005044:hydroxyethyl methacrylate