title: Microcirculation disturbance affects rats with acute severe pancreatitis following lung injury.
abstract: OBJECTIVE
  To study the effects of microcirculation disturbance (MD) on rats with acute severe pancreatitis (ASP).
  
  
  METHODS
  We developed ASP rat models, and anatomized separately after 1, 3, 5, 7, and 9 h. We took out blood and did hemorrheologic examination and erythrocyte osmotic fragility test, checked up the water content, capillary permeability, and genetic expression of intercellular adhesion molecule-1 (ICAM-1) in lung tissues, examined the apoptosis degree of blood vessel endothelium while we tested related gene expression of Bax and Bcl-2 in lung tissues. We did the same examination in control group.
  
  
  RESULTS
  The viscosity of total blood and plasma, the hematocrit, and the erythrocyte osmotic fragility were all increased. Fibrinogen was decreased. The water content in lung tissues and capillary permeability were increased. Apoptosis degree of blood vessel endothelium was increased too. ICAM-1 genetic expression moved up after 1 h and reached its peak value after 9 h.
  
  
  CONCLUSIONS
  MD plays an important role in ASP following acute lung injury (ALI). The functional damage of blood vessel endothelium, the apoptosis of capillary vessel endothelium, WBC edging-concentration and the increasing of erythrocyte fragility are the main reasons of ALI.
authors.affiliation: Department of Hepatobiliary Surgery, Xi'an Jiaotong University First Hospital, Xi'an 710061, Shaanxi Province, China. a1090224@pub.xaonline.com
authors.forename: Xue-Min
authors.initials: XM
authors.lastname: Liu
authors.forename: Qing-Guang
authors.initials: QG
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Cheng-En
authors.initials: CE
authors.lastname: Pan
mesh_terms: D000208:Acute Disease; D000818:Animals; D008833:Microcirculation; D010195:Pancreatitis / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D011652:Pulmonary Circulation; D051381:Rats; D012128:Respiratory Distress Syndrome / Q000150:complications* / Q000473:pathology / Q000503:physiopathology*; D012720:Severity of Illness Index