Arrangement of phosphatidylserine and phosphatidylethanolamine in the erythrocyte membrane.
Cross-linking of phosphatidylethanolamine and phosphatidylserine in the erythrocyte membrane with the reagent difluorodinitrobenzene was studied as a function of temperature, time and concentration of difluorodinitrobenzene. The optimal extent of cross-linking of phosphatidylethanolamine to phosphatidylethanolamine, phosphatidylethanolamine to phosphatidylserine and phosphatidylserine to phosphatidylserine was expressed as molar ratios of these three different cross-linked species. The experimental results were compared to different models of a phospholipid monolayer containing phosphatidylethanolamine and phosphatidylserine in which phosphatidylserine was arranged primarily as singles (having 6 phosphatidylethanolamine neighbors) as clusters of dimers, trimer and tetramers or as large clusters. In the various model monolayers each lipid component has 6 neighbors. The models which are consistent with the experimental results are those in which phosphatidylserine and phosphatidylethanolamine occur as small clusters in a non-random array.
Marinetti
G V
GV
Department of Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.
D003432:Cross-Linking Reagents / Q000494:pharmacology; D019281:Dimerization; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010718:Phosphatidylserines / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D003432:Cross-Linking Reagents; D008051:Lipid Bilayers; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010743:Phospholipids; C483858:phosphatidylethanolamine; D004139:Dinitrofluorobenzene