title: [HLA-G1 molecule expressed by ECV304 cells inhibit cytotoxic activity of allogeneic NK cells].
abstract: OBJECTIVE
  To study the effect of HLA-G1 molecule expressed by an endothelial cell line (ECV304) on the cytotoxic activity of allogeneic NK cells.
  
  
  METHODS
  ECV304 cells were transfected with recombinant plasmid pcDNA3-HLA-G1 by the liposome transfection, and the expressed HLA-G1 on the cell surface was detected by indirect immunofluorescent assay and flow cytometry. The cytotoxic activity of allogeneic NK cells against ECV304 cells was analyzed by the MTT method.
  
  
  RESULTS
  HLA-G1 was expressed on the surface of the transfected ECV304 cells. The specific lysis of NK cells against plasmid pcDNA3 transfected ECV304 was (50.6+/-18.1)%, while the specific lysis against pcDNA3-HLA-G1 transfected ECV304 was (29.7+/-11.4)%, which was significantly lower than the former (P<0.001).
  
  
  CONCLUSIONS
  HLA-G1 expressed by the ECV304 cells can inhibit cytotoxicity of allogeneic NK cells.
authors.affiliation: Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Cai-e
authors.initials: CE
authors.lastname: Zhang
authors.forename: Jun-yan
authors.initials: JY
authors.lastname: Han
authors.forename: Hui-jun
authors.initials: HJ
authors.lastname: Yang
authors.forename: Xiong-wen
authors.initials: XW
authors.lastname: Wu
authors.forename: Ya-fei
authors.initials: YF
authors.lastname: Huang
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Liang
authors.forename: Fei-li
authors.initials: FL
authors.lastname: Gong
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D005822:Genetic Vectors / Q000235:genetics; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology* / Q000502:physiology*; D008081:Liposomes; D014162:Transfection
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D008081:Liposomes