Gastrointestinal beta2microglobulin amyloidosis in hemodialysis patients: biochemical analysis of amyloid proteins in small formalin-fixed paraffin-embedded tissue specimens.
We present here a first report on the biochemical analysis of intestinal amyloid deposits found in two cases of hemodialysis-related amyloidosis. A new microtechnique was applied for extraction and immunochemical/chemical characterization of amyloid proteins in small amounts of fixed tissue, thus allowing precise identification of beta2microglobulin amyloid (Abeta2M) in both cases studied. The molecular mass of the identified amyloid beta2M was close to that of intact beta2M (12 kDa), with no evidence of the products of proteolytic fragmentation of these molecules. The isoelectrofocusing of the purified Abeta2M demonstrated a shift to more acidic pI as compared to the normal beta2M analyzed under the same experimental conditions. The obtained data suggest that the intestinal amyloid deposits in dialysis-related amyloidosis contain disease-specific beta2M isoforms, which could play a role in the pathogenesis of amyloid disease. The new methodology used might be useful in obtaining precise diagnosis of amyloidosis that is necessary for appropriate therapy, and also provide new important information on the chemical structure of amyloid proteins.
Kaplan
Batia
B
Heller Institute of Medical Research, Sheba Medical Center, Tel-Hashomer, Israel. kaplanb@sheba.health.gov.il
Martin
Brian M
BM
Livoff
Alejandro
A
Yeremenko
Dimitri
D
Livneh
Avi
A
Cohen
Hector I
HI
D000368:Aged; D000682:Amyloid / Q000737:chemistry / Q000378:metabolism*; D000686:Amyloidosis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D015153:Blotting, Western; D003082:Colectomy; D003108:Colonic Diseases / Q000209:etiology / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D004591:Electrophoresis, Polyacrylamide Gel; D017809:Fatal Outcome; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008875:Middle Aged; D006435:Renal Dialysis / Q000009:adverse effects*; D020539:Sequence Analysis, Protein; D001613:beta 2-Microglobulin / Q000276:immunology / Q000378:metabolism*
D000682:Amyloid; D001613:beta 2-Microglobulin