Codon 12 region of mouse K-ras gene is the site for in vitro binding of transcription factors GATA-6 and NF-Y.
Codon 12 of the K-ras gene is a generally recognized example of a mutational hot spot. By the approach of gel retardation and specific antibodies, a double-stranded oligonucleotide corresponding to the codon 12 region of the mouse K-ras gene (from 20 to 50 bp with respect to the exon 1 start) was found to be a site for cooperative binding of the transcription factors GATA-6 and NF-Y. GATA-6 and NF-Y were selectively activated with lung carcinogens 3-methylcholanthrene and nitrosoethylurea in mice of strains susceptible to lung tumorigenesis but not in animals of resistant strains. The interaction of GATA-6 and NF-Y with the codon 12 region of the K-ras gene is suggested to be involved in the mechanism of lung carcinogenesis.
Gorshkova
E V
EV
Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. schly@mail.ru
Kaledin
V I
VI
Kobzev
V F
VF
Merkulova
T I
TI
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D023081:CCAAT-Binding Factor / Q000378:metabolism*; D003062:Codon / Q000235:genetics*; D005038:Ethylnitrosourea / Q000737:chemistry; D050993:GATA6 Transcription Factor / Q000378:metabolism*; D011905:Genes, ras / Q000235:genetics*; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D008748:Methylcholanthrene / Q000737:chemistry; D051379:Mice
D023081:CCAAT-Binding Factor; D003062:Codon; D050993:GATA6 Transcription Factor; D008748:Methylcholanthrene; D005038:Ethylnitrosourea