title: Detection of chlorinated DNA and RNA nucleosides by HPLC coupled to tandem mass spectrometry as potential biomarkers of inflammation.
abstract: Upon inflammation, activated neutrophils secrete myeloperoxidase, an enzyme able to generate hypochlorous acid (HOCl) from hydrogen peroxide and chloride ions. An analytical method, involving HPLC coupled to electrospray tandem mass spectrometry, has been set-up to detect low levels of HOCl-induced nucleic acids lesions, including both ribo and 2'-deoxyribonucleoside derivatives of 8-chloroguanine, 8-chloroadenine and 5-chlorocytosine. Validation of the developed method was achieved using isolated cells treated with HOCl. The method was found to be sensitive enough to allow the measurement of background levels of 5-chloro-2'-deoxycytidine in the DNA of human white blood cells isolated from 7 mL of blood.
authors.affiliation: Laboratoire des Lésions des Acides Nucléiques, DRFMC/SCIB CEA Grenoble, 17 rue des Martyrs, F-38054 Grenoble Cedex 9, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Badouard
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Masuda
authors.forename: Hoyoku
authors.initials: H
authors.lastname: Nishino
authors.forename: Jean
authors.initials: J
authors.lastname: Cadet
authors.forename: Alain
authors.initials: A
authors.lastname: Favier
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Ravanat
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000032:analysis; D015415:Biomarkers / Q000032:analysis*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D003839:Deoxyadenosines / Q000032:analysis; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000032:analysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D007249:Inflammation / Q000175:diagnosis*; D007962:Leukocytes / Q000737:chemistry; D009705:Nucleosides / Q000032:analysis*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: C506093:8-chloro-2'-deoxyguanosine; C507300:8-chloroadenine; C479978:8-chlorodeoxyadenosine; C507301:8-chloroguanine; D015415:Biomarkers; D003839:Deoxyadenosines; D009705:Nucleosides; D003841:Deoxycytidine; C035826:5-chloro-2'-deoxycytidine; D006147:Guanine; D003596:Cytosine; D003849:Deoxyguanosine; D000225:Adenine; C401182:5-chlorocytosine