title: Intestinal microdialysis--applicability, reproducibility and local tissue response in a pig model.
abstract: OBJECTIVE
  Microdialysis has been applied to the intestinal wall for the purpose of monitoring local ischemia. The aim of this study was to investigate the applicability, reproducibility and local response to microdialysis in the intestinal wall.
  
  
  METHODS
  In 12 pigs two microdialysis probes were inserted into the ileal wall, one in the peritoneal cavity and one in the psoas muscle. Relative recovery was measured for all probes by the no net flux method. Metabolic measurements of glucose, lactate and glycerol were performed over six hours. The ileal wall segments containing the probes were processed for histological examination.
  
  
  RESULTS
  Large intra- and inter-group differences in the relative recovery were found between all locations. Absolute values of metabolites showed no significant changes during the study period. The lactate in blood was 25-30% of the intra-tissue values. A severe inflammatory reaction was seen in the ileal wall around all probes.
  
  
  CONCLUSIONS
  Measurement of the relative recovery is essential for valid measurements of metabolites when using microdialysis. The inflammatory reaction around the probe in the intestinal wall is likely to affect metabolism and measurements hereof. Therefore intestinal wall microdialysis seems confined to experimental research, and future studies should consider the intra-peritoneal approach.
authors.affiliation: Clinical Institute, Aarhus University Hospital, Skejby Hospital, Aarhus N, Denmark. kje@studmed.au.dk
authors.forename: K J
authors.initials: KJ
authors.lastname: Emmertsen
authors.forename: P
authors.initials: P
authors.lastname: Wara
authors.forename: F B
authors.initials: FB
authors.lastname: Soerensen
authors.forename: L B
authors.initials: LB
authors.lastname: Stolle
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000737:chemistry; D003956:Dialysis / Q000379:methods*; D005260:Female; D005947:Glucose / Q000032:analysis; D007082:Ileum / Q000098:blood supply* / Q000378:metabolism; D007511:Ischemia / Q000175:diagnosis* / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis; D023421:Models, Animal; D013552:Swine
chemical_list: D019344:Lactic Acid; D005947:Glucose