title: Ordered differential display.
abstract: Ordered differential display (ODD) is one of the approaches that uses systematic, rather than random, sampling of transcripts for display and thereby provides means to browse through essentially all the transcripts in the compared mRNA pools. It is specifically adapted for small amounts of starting material. The protocol outlined here, in addition to ODD procedure itself, also describes isolation of RNA and synthesis of double-stranded cDNA from small biological samples.
authors.affiliation: Whitney Laboratory for Marine Biology, University of Florida, St. Augustine, USA.
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Matz
authors.forename: Ella A
authors.initials: EA
authors.lastname: Meleshkevitch
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D004586:Electrophoresis; D020869:Gene Expression Profiling / Q000379:methods*; D005821:Genetic Techniques; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D009841:Oligonucleotides; D012333:RNA, Messenger; D012313:RNA