Validation of HPLC analysis method of a novel antihypertensive agent MS23 in rat plasma.
MS23 is a vasodilator with unique dual action pharmacological profile to inhibit type 4 PDE and antagonize L-type calcium channels. We validated an analytical protocol for MS23 in rat plasma using high performance liquid chromatography (HPLC). A C18 column and a phosphate/acetonitrite buffer were used for chromatographic separation. UV detection was performed at 307 nm. The calibration curve for MS23 was linear in the range from 50 to 10,000 ng/ml. The limit of quantification (LOQ) was 50 ng/ml. The results demonstrate that the method has linearity (R = 0.9989), specificity, and acceptable precision/accuracy. This method is simple, economic, and sufficient for in vivo pharmacokinetic studies on the compound.
Wang
Ting
T
Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of South Carolina, Columbia, 29208, USA.
Fox
Laura M
LM
Wang
Desuo
D
D000818:Animals; D000959:Antihypertensive Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D011758:Pyrroles / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000959:Antihypertensive Agents; C508412:MS23 agent; D011758:Pyrroles