title: A sulfonated capillary that gives reproducible migration times for capillary zone electrophoresis and micellar electrokinetic chromatography.
abstract: To obtain reproducible migration times and rapid analyses of analytes, sulfonate groups were chemically introduced to the inner wall of untreated fused-silica capillary with 2-(4-chlorosulfonylphenyl)ethyltrichlorosilane. The sulfonated capillary showed relatively constant electroosmotic mobility which was greater than that obtained by an untreated fused-silica capillary over the pH range studied (pH 2-9). In both CZE and MEKC, the RSDs of the migration times of analytes with the sulfonated capillary were less than 0.2% which were significantly lower than those obtained with an untreated fused-silica capillary (0.5-3.5%). When BGE were set at pH 7.0 for CZE and MEKC, the analysis times with the sulfonated capillary were about half those obtained with an untreated fused-silica capillary. These results indicate that the sulfonated capillary can provide highly reproducible and rapid analyses in CE.
authors.affiliation: Toyama Institute of Health, Kosugi-machi, Toyama, Japan. shuji.kodama@pref.toyama.lg.jp
authors.forename: Shuji
authors.initials: S
authors.lastname: Kodama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Terashima
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Honda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Taga
authors.forename: Susumu
authors.initials: S
authors.lastname: Honda
mesh_terms: D000146:Acids, Carbocyclic / Q000302:isolation & purification; D000577:Amides / Q000302:isolation & purification; D001190:Arylsulfonates; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D017640:Silicates
chemical_list: D000146:Acids, Carbocyclic; D000577:Amides; D001190:Arylsulfonates; D017640:Silicates