Chromosome rearrangements in canine fibrosarcomas.
We have previously reported the use of six- and seven-color paint sets in the analysis of canine soft tissue sarcomas. Here we combine this technique with flow sorting of translocation chromosomes, reverse painting, and polymerase chain reaction (PCR) analysis of the gene content of the reverse paint in order to provide a more detailed analysis of cytogenetic abnormalities in canine tumors. We examine two fibrosarcomas, both from female Labrador retrievers, and show abnormalities in chromosomes 11 and 30 in both cases. Evidence of involvement of TGFBR1 is presented for one tumor.
Sargan
D R
DR
Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK. drs20@cam.ac.uk
Milne
B S
BS
Hernandez
J Aguirre
JA
O'Brien
P C M
PC
Ferguson-Smith
M A
MA
Hoather
T
T
Dobson
J M
JM
D030201:Activin Receptors, Type I / Q000235:genetics; D000818:Animals; D019457:Chromosome Breakage / Q000235:genetics*; D020223:Chromosome Painting / Q000379:methods / Q000662:veterinary; D033481:Chromosomes, Mammalian / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005260:Female; D005354:Fibrosarcoma / Q000235:genetics / Q000662:veterinary*; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D007621:Karyotyping / Q000662:veterinary; D008677:Metaphase / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics; D012983:Soft Tissue Neoplasms / Q000235:genetics / Q000662:veterinary*; D014178:Translocation, Genetic / Q000235:genetics*
D017931:DNA Primers; D018125:Receptors, Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D030201:Activin Receptors, Type I; D000077293:Receptor, Transforming Growth Factor-beta Type I