title: [Influence of aerosols on the expression of cyclin B1, cyclin C and proliferating cell nuclear antigen in wound tissue healing of burned rat models].
abstract: OBJECTIVE
  To investigate the influence of aerosols on the expression of cyclin B(1), cyclin C and proliferating cell nuclear antigen (PCNA) in wound tissue healing of burned rat models.
  
  
  METHODS
  Sprague Dawley (SD) rats were inflicted as the deep partial thickness burn models. Rats were randomly divided into experimental group and control group. The experimental group were treated with aerosols. Samples were collected in 1 approximately 10 postburn days. Immunohistochemistry and image analysis methods were conducted to examine the expression of cyclin B(1), cyclin C and PCNA in both experimental and control groups.
  
  
  RESULTS
  The expression of cyclin C in experimental group was detected in nucleus of skin basal cell on the second postburn day, increased evidently at the fifth days and sustained at high expression level up to the tenth days after treatment. The expression of cyclin C in experimental group was significantly higher than control group (P < 0.05). The expression of PCNA was first observed in skin basal cell nucleus and hair follicle cell nucleus in both experimental and control group on the third postburn day. The expression of PCNA increased evidently at the fifth days in experimental after treatment and that increased evidently at the seventh days in control group, which showed there were lots of active proliferation cell. And the difference of the expression of PCNA between experimental and control group was significant (P < 0.01). The expression of cyclin B(1) was detected in nucleus and cytoplasm of skin basal cell in both groups on the third postburn day, and no difference between the experimental and control group (P > 0.05).
  
  
  CONCLUSIONS
  Aerosols can up-regulate the expression of cyclin C and PCNA in skin basal cell nucleus. Therefore the aerosols can accelerate wound tissue healing.
authors.affiliation: Department of Burns, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Tian-zeng
authors.initials: TZ
authors.lastname: Li
authors.forename: Ying-bin
authors.initials: YB
authors.lastname: Xu
authors.forename: Shao-hai
authors.initials: SH
authors.lastname: Qi
authors.forename: Hui-zhen
authors.initials: HZ
authors.lastname: Liang
authors.forename: Ju-lin
authors.initials: JL
authors.lastname: Xie
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Chen
authors.forename: Shu-sen
authors.initials: SS
authors.lastname: Zheng
mesh_terms: D000336:Aerosols; D000818:Animals; D002056:Burns / Q000378:metabolism / Q000628:therapy*; D019926:Cyclin B / Q000096:biosynthesis*; D056744:Cyclin B1; D056745:Cyclin C; D016213:Cyclins / Q000096:biosynthesis*; D004195:Disease Models, Animal; D004599:Electric Stimulation Therapy / Q000379:methods*; D005260:Female; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000336:Aerosols; C534701:Ccnb1 protein, rat; C503676:Ccnc protein, rat; D019926:Cyclin B; D056744:Cyclin B1; D056745:Cyclin C; D016213:Cyclins; D018809:Proliferating Cell Nuclear Antigen