[Study on proliferation effect of extracts of Piper longum on mesenchymal stem cells of rat bone marrow and the relationship to chemical functional groups].
OBJECTIVE
To observe the effect of volatile oil and aqueous soluable part of Piper longum on proliferation of rat mesenchymal stem cell (MSC) and the chemical functional groups.


METHODS
Mesenchymal stem cells were dissociated from rat bone marrow and marked by Brdu, and the expression of CD44, CD54 and double label of Brdu and CD44. The growth of rat mesenchymal stem cell under volatile oil and aqueous soluable part of Piper longum was observed by means of cell viability measurement (MTT) and morphological observation and Brdu, PCNA immunohistochemical methods.


RESULTS
Volatile oil of Piper longum could promote the cell viability of MSC and the number of Brdu, PCNA positive cell in dose-dependant. There was significant difference in comparision with control groups, C = C(50.66%), -OH(27.02%) and other functional groups in volatile oil of Piper longum were determined by GC-MS, but the aqueous soluable part of Piper longum could not promote the proliferation of MSC.


CONCLUSIONS
Volatile oil of Piper longum which consists of C = C, -OH, and other functional groups has strong effect on enhancing proliferation of MSC.
Li
Xican
X
Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405.
Zhou
Jianhong
J
Li
Hui
H
Du
Shaohui
S
Li
Yiwei
Y
Huang
Ling
L
Chen
Dongfeng
D
D000818:Animals; D015415:Biomarkers; D001853:Bone Marrow; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007150:Immunohistochemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D015394:Molecular Structure; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031701:Piper / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile