Murine antigen-presenting cells are multifunctional in vitro biosensors for detecting the immunoactive potential of bovine milk products.
Antigen-presenting cells (APCs) are multifunctional components of the immune defense system. In this study, murine APCs were used as biosensors to detect immunologically active components of bovine milk and colostrum. By measuring changes in cell surface protein markers [major histocompatibility complex II, cluster designation (CD)40, CD86] and cytokines (tumor necrosis factor-alpha and interleukin-10) associated with APC activation, we identified a number of compounds that are immunoactive. The mouse macrophage cell line MH-S offered a simple and robust target for identification of immunoactives. The assay was shown to be adaptable for measuring immunoenhancing or immunosuppressive substances. Large-scale screening of milk extracts using this bioassay has the potential to identify substances that could be developed into nutraceuticals or pharmaceutical-grade immunotherapeutics.
Davies
Corinne
C
Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
McConnell
Michelle
M
Slobbe
Lynn
L
Haggarty
Neill
N
Buchan
Glenn
G
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D051940:B7-2 Antigen / Q000032:analysis; D015374:Biosensing Techniques; D019013:CD40 Antigens / Q000032:analysis; D002417:Cattle; D002460:Cell Line; D003126:Colostrum / Q000737:chemistry; D003470:Culture Media; D003713:Dendritic Cells; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D016753:Interleukin-10 / Q000032:analysis; D008262:Macrophage Activation; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D008892:Milk / Q000737:chemistry / Q000276:immunology*; D008894:Milk Proteins; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D051940:B7-2 Antigen; D019013:CD40 Antigens; D003470:Culture Media; D000949:Histocompatibility Antigens Class II; D008894:Milk Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10