Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells.
OBJECTIVE
To study the function of alpha-fetoprotein (AFP) in SMMC-7721 hepatoma cells.


METHODS
A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed and transfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its effect on SMMC-7721 cell proliferation and cell death was detected by MTT and fluorescence activated cell sorter (FACS).


RESULTS
The AFP-siRNA expressing plasmid downregulated the expression of AFP obviously (about 34%), and inhibited SMMC-7721 cell proliferation, but did not induce apoptosis.


CONCLUSIONS
Downregulation of AFP siRNA inhibits proliferation of SMMC-7721 cells, but cannot cause apoptosis.
Wang
Yun-Shan
YS
Jinan Central Hospital, 105# Jinan Jiefang Road, Jinan 250013, Shandong Province, China. sdjnwys@163.com
Ma
Xiao-Li
XL
Qi
Tong-Gang
TG
Liu
Xiang-Dong
XD
Meng
Yue-Sheng
YS
Guan
Guang-Ju
GJ
D017209:Apoptosis; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D000509:alpha-Fetoproteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology
D034741:RNA, Small Interfering; D000509:alpha-Fetoproteins