Antinociceptive action of the extract and the flavonoid quercitrin isolated from Bauhinia microstachya leaves.
This study examined the antinociceptive effect of Bauhinia microstachya (Leguminosae), a native plant widely distributed in the South of Brazil, in several chemical and mechanical models of pain. The methanolic extract (ME) from B. microstachya (3--30 mg kg(-1), i.p.) and the isolated compound quercitrin (1--10 mg kg(-1), i.p.), given 30 min earlier, produced a dose-dependent inhibition of acetic-acid-induced visceral pain in mice, with a mean ID50 value (dose necessary to reduce the nociceptive response by 50% relative to the control value) of 7.9 and 2.4 mg kg(-1), respectively. The ME of B. microstachya (3--100 mg kg(-1), i.p., 30 min earlier) also caused a dose-dependent inhibition of capsaicin-induced pain, with a mean ID50 value of 18.8 mg kg(-1). Moreover, the ME (3--100 mg kg(-1), i.p., 30 min earlier) produced marked inhibition of both phases of formalin-induced pain, with mean ID50 values for the neurogenic and the inflammatory phases of 30.3 and 17.2 mg kg(-1), respectively. In addition, the ME of B. microstachya (3--300 mg kg(-1), i.p., 30 min earlier) inhibited, in a graded manner, the hyperalgesia induced by bradykinin (3.2 microg/paw), substance P (13.5 microg/paw), carrageenan (300 microg/paw), capsaicin (100 microg/paw) and adrenaline (100 ng/paw) in the rat paw, with mean ID50 values of 20.5, 17.9, 101.8, 54.2 and 99.7 mg kg(-1), respectively. Taken together, these data demonstrate that ME of B. microstachya elicited a pronounced antinociceptive action against several chemical and mechanical models of pain in mice and rats. The precise mechanism responsible for the antinociceptive effect of the extract still remains unclear, but seems to be partly related to modulation of the release or action of pro-inflammatory mediators involved in the models of pain used. Finally, the flavonoid quercitrin isolated from this plant appears to contribute for the antinociceptive property of the methanolic extract.
Gadotti
Vinícius M
VM
Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, Florianópolis 88040-900, SC, Brazil.
Schmeling
Leonardo O
LO
Machado
Cláudia
C
Liz
Fernanda H
FH
Filho
Valdir Cechinel
VC
Meyre-Silva
Christiane
C
Santos
Adair R S
AR
D000009:Abdominal Muscles / Q000187:drug effects / Q000503:physiopathology; D019342:Acetic Acid; D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D036481:Bauhinia / Q000737:chemistry*; D002351:Carrageenan; D003251:Constriction, Pathologic / Q000139:chemically induced / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D005557:Formaldehyde; D006930:Hyperalgesia / Q000139:chemically induced / Q000517:prevention & control; D007274:Injections, Intraperitoneal; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control; D010147:Pain Measurement / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000700:Analgesics; D010936:Plant Extracts; D005557:Formaldehyde; C012526:quercitrin; D002351:Carrageenan; D011794:Quercetin; D019342:Acetic Acid