title: Resveratrol may reduce apoptosis of rat testicular germ cells after experimental testicular torsion.
abstract: The aim of the study is to evaluate the effects of resveratrol on apoptosis of testicular germ cells after experimental testicular torsion. Thirty Wistar albino rats were divided into 3 groups. Torsions were created by rotating the right testis 720 degrees in a clockwise direction for 4 h in all groups except the control group (group 1). They were then repaired by counter-rotation and replaced into the scrotum. In group 2, saline was infused 30 min before detorsion. In group 3, 30 mg/kg resveratrol was infused 30 min before detorsion. In groups 2 and 3, the bilateral testes were removed to determine germ cell apoptosis after 20 h of detorsion. The number of apoptotic cells was evaluated using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) technique and caspase 3. Mean apoptotic score of ipsilateral testes in group 3 was lower than that of group 2 (p < 0.05). Mean apoptotic score of the contralateral testes in group 3 was not different from that of group 2 (p > 0.05). The present study demonstrates that intraperitoneal administration of resveratrol in rats may decrease germ cell apoptosis in the ipsilateral testes.
authors.affiliation: Department of Pediatric Surgery, Turgut Ozal Medical Center, Inonu University, Faculty of Medicine, Malatya, Turkey. suguralp@inonu.edu.tr
authors.forename: S
authors.initials: S
authors.lastname: Uguralp
authors.forename: U
authors.initials: U
authors.lastname: Usta
authors.forename: B
authors.initials: B
authors.lastname: Mizrak
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013086:Spermatic Cord Torsion / Q000188:drug therapy* / Q000601:surgery; D013094:Spermatozoa / Q000187:drug effects*; D018709:Statistics, Nonparametric; D013267:Stilbenes / Q000494:pharmacology*; D013737:Testis / Q000473:pathology
chemical_list: D013267:Stilbenes; D000077185:Resveratrol