[Study of HPLC chromatographic fingerprint of Citrus reticulata Blanco cv. Dahongan].
OBJECTIVE
To establish HPLC chromatographic fingerprint of Citrus reticulata Blanco cv. Dahongan.


METHODS
The experimental condition of the RP-HPLC method was as follows: Angilent Zorbax C18 column (4.6 mm x 250 mm, 5 microm), with linear gradient elution using (A) methanol and (B) water; The flow rate was 1.0 ml/min, and the injected volume was 20 microl, detected wavelength was 283 nm.


RESULTS
Under the qualitative conditions we determined different samples of Citrus reticulata Blanco cv. Dahongan. There were 11 common peaks in chromatographic.


CONCLUSIONS
According to analyze and contrast the relative retention values and relative peak area in chromatographic fingerprint, we establish HPLC chromatographic fingerprint of Citrus reticulata Blanco cv. Dahongan to provide a scientific basement for the quality evaluation of it.
Xu
Runjuan
R
Jiangmen Institute for Drug Control, Jiangmen 529000.
Zhang
Fanghong
F
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D005638:Fruit / Q000737:chemistry; D006569:Hesperidin / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D006569:Hesperidin