Competitive binding assay for glucose based on glycodendrimer-fluorophore conjugates.
A new fluorescent glucose assay has been created using Alexa Fluor 647-labeled concanavalin A (Con A) and a fourth-generation PAMAM Alexa Fluor 594-labeled glycodendrimer. This assay has been shown to have a large response to glucose within the biological range and to be capable of functioning within a polymer hydrogel. In this paper, the glucose response is shown to be a single fluorophore-based quenching reaction. Data showing that the sensor is fully reversible and specific through competitive binding between the dendrimer and glucose with Con A are presented. Overall, the assay is shown to have potential over the traditional dextran-based assay because it has a larger dynamic response to physiological glucose concentrations, incorporates longer wavelength dyes that improve signal penetration through dermal tissue, and provides an internal reference in the form of a nonreactive fluorescent label.
Ibey
Bennett L
BL
Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, USA. bli6339@tamu.edu
Beier
Hope T
HT
Rounds
Rebecca M
RM
Coté
Gerard L
GL
Yadavalli
Vamsi K
VK
Pishko
Michael V
MV
D001667:Binding, Competitive; D003208:Concanavalin A / Q000737:chemistry; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000737:chemistry; D005947:Glucose / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry; D012680:Sensitivity and Specificity
C417664:Alexa594; D009930:Organic Chemicals; D003208:Concanavalin A; C067297:8-aminohexylamino cAMP; D000242:Cyclic AMP; D005947:Glucose