The effect of calcium chelating or binding agents on Candida albicans.
OBJECTIVE
The aim of this study was to evaluate antifungal effects of calcium-chelating or -binding agents on Candida albicans comparing with conventional antifungal agents.


METHODS
Two clinical oral isolates and 1 standard strain of C albicans were included in the study. Test solutions were ethylenediamine-tetraacetic acid (EDTA), ethyleneglycol-tetraacetic acid (EGTA), sodium fluoride (NaF), titanium tetrafluoride (TiF4), nystatin, and ketoconazole. Minimum inhibitory and fungicidal concentrations of the solutions were determined. The results were analyzed statistically using Friedman's nonparametric 2-way ANOVA and Mann-Whitney U tests.


RESULTS
All isolates demonstrated similar susceptibility patterns (P>.05). Except ketoconazole, EDTA had the highest antifungal and fungicidal activity, followed by TiF4. EGTA and NaF were the weakest agents against C albicans among all test solutions.


CONCLUSIONS
EDTA and TiF4 may be recommended as an alternative irrigating solution particularly in persistent root canal infections and in root canals of patients having a high incidence of oral candidosis.
Ates
Mustafa
M
Department of Biology, Division of Basic and Industrial Microbiology, School of Science, Ege University, Izmir, Turkey.
Akdeniz
Bedriye Güniz
BG
Sen
Bilge Hakan
BH
D000704:Analysis of Variance; D000935:Antifungal Agents / Q000494:pharmacology*; D002118:Calcium / Q000737:chemistry; D002176:Candida albicans / Q000187:drug effects*; D002614:Chelating Agents / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D007654:Ketoconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009761:Nystatin / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012969:Sodium Fluoride / Q000494:pharmacology; D018709:Statistics, Nonparametric; D014025:Titanium / Q000494:pharmacology
D000935:Antifungal Agents; D002614:Chelating Agents; D012388:Root Canal Irrigants; D009761:Nystatin; D004533:Egtazic Acid; D012969:Sodium Fluoride; D004492:Edetic Acid; D014025:Titanium; D005459:Fluorides; D007654:Ketoconazole; D002118:Calcium; C032240:titanium tetrafluoride