Correlation of HSP70 expression and cell viability following thermal stimulation of bovine aortic endothelial cells.
Thermal preconditioning protocols for cardiac cells were identified which produce elevated HSP70 levels while maintaining high cell viability. Bovine aortic endothelial cells were heated with a water bath at temperatures ranging from 44 to 50 degrees C for periods of 1-30 min. Thermal stimulation protocols were determined which induce HSP70 expression levels ranging from 2.3 to 3.6 times the control while maintaining cell viabilities greater than 90%. An Arrhenius injury model fit to the cell damage data yielded values of A = 1.4 X 10(66) s(-1) and Ea = 4.1 X 10(5) J/mol. Knowledge of the injury parameters and HSP70 kinetics will enhance dosimetry guideline development for thermal stimulation of heat shock proteins expression in cardiac tissue.
Rylander
Marissa Nichole
MN
The University of Texas at Austin, Biomedical Engineering, 1 University Station C0800, Austin, TX 78712-0238, USA. n.forney@mail.utexas.edu
Diller
Kenneth R
KR
Wang
Sihong
S
Aggarwal
Shanti J
SJ
D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000502:physiology; D017209:Apoptosis / Q000502:physiology*; D002417:Cattle; D002470:Cell Survival / Q000502:physiology*; D002478:Cells, Cultured; D003198:Computer Simulation; D042783:Endothelial Cells / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response / Q000502:physiology*; D006358:Hot Temperature; D008954:Models, Biological; D013223:Statistics as Topic
D018840:HSP70 Heat-Shock Proteins