title: In vivo single-shot, proton-localized 13C MRS of rhesus monkey brain.
abstract: A single-shot, proton-localized, polarization transfer (13)C spectroscopic method was proposed and implemented on a 4.7 T scanner for studying rhesus monkey brains. The polarization transfer sequence was mostly adiabatic, minimizing signal loss due to B(1) inhomogeneity. RF pulses in polarization transfer were also used for voxel selection of protons with gradient fields. The transferred (13)C magnetization was refocused by additional refocusing adiabatic pulses. With the intravenous infusion of D-[1-(13)C]glucose solution, (13)C NMR spectra from a 30 mL voxel were acquired for the resonances of C1 of glucose, C2,3,4 of glutamate and glutamine. The time-resolved turnover of glutamate, glutamine and aspartate from intravenously infused D-[1-(13)C]glucose at a temporal resolution of 12 min was demonstrated with excellent spectral resolution and signal-to-noise ratio. Typically, the half-height linewidth of the decoupled (13)C peaks was approximately 4 Hz. Data obtained with infusion of sodium [2-(13)C]acetate using the proposed polarization transfer method and data from the carboxylic carbon region using non-localized acquisition are also presented.
authors.affiliation: National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Shizhe
authors.initials: S
authors.lastname: Li
authors.forename: Zhengguang
authors.initials: Z
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Martin
authors.initials: M
authors.lastname: Lizak
authors.forename: John
authors.initials: J
authors.lastname: Bacher
authors.forename: Robert B
authors.initials: RB
authors.lastname: Innis
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D008253:Macaca mulatta; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D011522:Protons
chemical_list: D002247:Carbon Isotopes; D011522:Protons; D005973:Glutamine; D005947:Glucose