title: DNA microarray-based detection of nosocomial pathogenic Pseudomonas aeruginosa and Acinetobacter baumannii.
abstract: Infection by nosocomial pathogenic bacteria is increasingly becoming a major threat to the patients in the hospital. We have developed a diagnostic DNA microarray for the detection of two important nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii. The diagnostic DNA microarray contains the species-specific probes of 15mer oligonucleotides designed based on the sequences of 23S ribosomal DNA. The performance of DNA microarray in diagnosing P. aeruginosa and A. baumannii was evaluated using reference bacteria as well as clinical specimens such as blood, stool, pus, sputum, urine and cerebrospinal fluid. Using this DNA microarray, A. baumannii could be successfully detected in 11 out of 13 clinical specimens, thus giving the sensitivity of 84.6% with the specificity of 100% and the positive predictive value of 100%. P. aeruginosa could also be detected in 25 out of 26 clinical specimens, showing the sensitivity of 96.2%, the specificity of 100%, and the positive predictive value of 100%. These results suggest that two nosocomial pathogens, P. aeruginosa and A. baumannii, can be efficiently diagnosed by using the DNA microarray developed in this study.
authors.affiliation: Department of Radiation Oncology, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Ki Chang
authors.initials: KC
authors.lastname: Keum
authors.forename: Seung Min
authors.initials: SM
authors.lastname: Yoo
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Chang
authors.forename: Nae Choon
authors.initials: NC
authors.lastname: Yoo
authors.forename: Won Min
authors.initials: WM
authors.lastname: Yoo
authors.forename: June Myung
authors.initials: JM
authors.lastname: Kim
authors.forename: Jun Yong
authors.initials: JY
authors.lastname: Choi
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Kim
authors.forename: Gene
authors.initials: G
authors.lastname: Lee
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics*; D003428:Cross Infection / Q000382:microbiology*; D006801:Humans; D025202:Molecular Diagnostic Techniques; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D015203:Reproducibility of Results