title: Slight increase of serum S-100B during porcine endotoxemic shock may indicate blood-brain barrier damage.
abstract: Septic shock is a condition that affects many organs, but little is known about the effects on the central nervous system. S-100B, an acidic low molecular weight protein, has attracted considerable interest as a marker for brain damage and disintegration of the blood-brain barrier. It is released into the cerebrospinal fluid and blood from brain tissue after brain damage. We studied S-100B in a porcine model of endotoxemic shock that resembles human Gram-negative septic shock. Ten piglets received IV endotoxin, and plasma samples were collected before the endotoxin infusion and each hour (1-6 h) during the endotoxin infusion. S-100B was measured by sandwich enzyme-linked immunosorbent assay. Low levels of plasma S-100B were detected, but there was a significant increase in S-100B during Hours 1-5 in comparison with the 0 values. We determined that endotoxemia causes a very small but significant increase in the levels of the widely used brain damage marker serum S-100B. However, it cannot be excluded that the increase in S-100B could be caused by release from organs other than the brain.
authors.affiliation: Departments of *Medical Sciences and †Surgical Sciences, Uppsala University Hospital, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Larsson
authors.forename: Miklós
authors.initials: M
authors.lastname: Lipcsey
authors.forename: Jan
authors.initials: J
authors.lastname: Sjölin
authors.forename: Lars-Olof
authors.initials: LO
authors.lastname: Hansson
authors.forename: Mats B
authors.initials: MB
authors.lastname: Eriksson
mesh_terms: D000818:Animals; D015415:Biomarkers; D001812:Blood-Brain Barrier / Q000503:physiopathology*; D019446:Endotoxemia / Q000097:blood* / Q000503:physiopathology; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D008297:Male; D009414:Nerve Growth Factors / Q000097:blood*; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000097:blood*; D012772:Shock, Septic / Q000097:blood* / Q000503:physiopathology; D013552:Swine
chemical_list: D015415:Biomarkers; D006454:Hemoglobins; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins