title: [The role of tissue factor in the invasion and metastasis of colorectal carcinoma cells].
abstract: OBJECTIVE
  To investigate the role of tissue factor (TF) in the invasion and hematogenous metastasis of human colorectal carcinoma cells.
  
  
  METHODS
  The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into HT-29 and LoVo cells by the way of lipofactamine 2000. TF proteins in transfected cells were detected by Western Blot. Then the transfected and un-transfected tumor cells were implanted into nude mice (Balb/c Nu/Nu) to produce primary tumor, lung metastasis and liver metastasis respectively.
  
  
  RESULTS
  HT-29 and LoVo cells with sense-TFcDNA transfection showed increased TF expression compared with the cells without transfection, but the cells with antisense-TFcDNA transfection got the contrary change. The primary tumor growth and invasive range, lung metastasis and live metastasis all increased in sense transfectants but reduced in antisense transfectants.
  
  
  CONCLUSIONS
  TF can increase the invasion and hematogenous metastatic ability of human colorectal carcinoma cells.
authors.affiliation: Department of General Surgery, First Hospital of Peking University, Beijing 100034, China.
authors.forename: Yuan-lian
authors.initials: YL
authors.lastname: Wan
authors.forename: Long
authors.initials: L
authors.lastname: Rong
authors.forename: Yu-cun
authors.initials: YC
authors.lastname: Liu
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Yao
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.forename: Yi-sheng
authors.initials: YS
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology*; D016373:DNA, Antisense / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D013925:Thromboplastin / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014162:Transfection
chemical_list: D016373:DNA, Antisense; D018076:DNA, Complementary; D013925:Thromboplastin