Arginine as an effective additive in gel permeation chromatography.
A major problem in gel permeation chromatography (GPC) or size exclusion chromatography is non-specific binding of applied proteins to the column matrix (stationary phase). We have tested an aqueous arginine solution as the GPC mobile phase on silica-based and polymer-based columns, using mouse monoclonal antibody and recombinant human activin, interleukin-6, basic fibroblast growth factor, and interferon-gamma as model proteins. We observed that addition of arginine to the mobile phase improves separation of the proteins and their soluble aggregates from the GPC columns, which suggests that arginine is an effective additive for the GPC mobile phase.
Ejima
Daisuke
D
Applied Research Department, AminoScience Laboratories, Ajinomoto Inc., Kawasaki 210-8681, Japan. daisuke_Ejima@ajinomoto.com
Yumioka
Ryosuke
R
Arakawa
Tsutomu
T
Tsumoto
Kouhei
K
D028341:Activins / Q000302:isolation & purification; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001120:Arginine / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods*; D016222:Fibroblast Growth Factor 2 / Q000302:isolation & purification; D006801:Humans; D007371:Interferon-gamma / Q000302:isolation & purification; D015850:Interleukin-6 / Q000302:isolation & purification; D051379:Mice; D011994:Recombinant Proteins / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D015850:Interleukin-6; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D028341:Activins; D007371:Interferon-gamma; D001120:Arginine