Inactivation of vascular smooth muscle cells photosensitised by liposome-delivered Zn(II)-phthalocyanine.
Photodynamic therapy is a promising approach for the prevention of arterial restenosis, which frequently occurs after balloon angioplasty, largely owing to abnormal proliferation of vascular smooth muscle cells (VSMC) and their migration from the media to the intima, where they originate intimal hyperplasia (IH). We investigated the efficacy of Zn(II)-phthalocyanine-photosensitised processes in promoting the inactivation of VSMC. This liposome delivered phthalocyanine is readily taken up by VSMC, largely partitions in the Golgi apparatus, and upon photoactivation causes >95% cell mortality using mild irradiation conditions (e.g. 5 min irradiation at 1 microM ZnPc). Cell death occurs through the parallel development of random necrotic and apoptotic processes.
Magaraggia
Michela
M
Dipartimento di Biologia, Università di Padova, Via U. Bassi 58/B, 35121 Padova, Italy. michela.magaraggia@unipd.it
Visonà
Adriana
A
Furlan
Anna
A
Pagnan
Antonio
A
Miotto
Giovanni
G
Tognon
Giuseppe
G
Jori
Giulio
G
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D006056:Golgi Apparatus / Q000378:metabolism; D006965:Hyperplasia / Q000188:drug therapy; D007211:Indoles / Q000008:administration & dosage / Q000494:pharmacology*; D054833:Isoindoles; D008081:Liposomes; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D009336:Necrosis / Q000378:metabolism; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors; D017539:Tunica Intima / Q000473:pathology; D017967:Zinc Compounds
D007211:Indoles; D054833:Isoindoles; D008081:Liposomes; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine