[Human recombinant antibodies to Ebola virus: preparation and characteristics].
Human recombinant antibodies against a purified Ebola virus (EV) lysate were selected from a combinatorial library of scFv-antibodies using the phage display technique. Nine unique antibodies were identified after sequencing the Vh- and Vl-genes encoding the selected antibodies. Solid-phase enzyme immunoassay (EIA) indicated that these antibodies were able to bind both inactivated and native EV. Immunoblotting showed that 6 antibodies identified nucleoprotein (NP), one antibody did VP24 and another antibody did VP40. One of the selected antibodies reacted with two EP proteins: VP24 and VP40. Solid-phase EIA demonstrated cross-reactivity with Marburg virus (MAR) and defined VP24 MAR as a target protein for the antibody.
Tikunova
N V
NV
Batanova
T A
TA
Chepurnov
A A
AA
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D000918:Antibody Specificity; D022801:Complementarity Determining Regions / Q000235:genetics; D003429:Cross Reactions; D029043:Ebolavirus / Q000276:immunology*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000276:immunology; D019151:Peptide Library; D017434:Protein Structure, Tertiary / Q000235:genetics; D014758:Viral Core Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
D000914:Antibodies, Viral; D022801:Complementarity Determining Regions; D007135:Immunoglobulin Variable Region; D009698:Nucleoproteins; D019151:Peptide Library; C446938:VP24 protein, Ebola virus; D014758:Viral Core Proteins; D014764:Viral Proteins; C084819:nucleoprotein VP40, Ebola virus