Sublethal toxicity of two wastewater treatment polymers to lake trout fry (Salvelinus namaycush).
Lake trout fry (Salvelinus namaycush) were exposed in laboratory experiments to two wastewater treatment polymers, one anionic (MagnaFloc 156) and one cationic (MagnaFloc 368; Ciba Speciality Chemical), to determine if these chemicals which are used and discharged by mining operations in Canada's North pose a significant hazard to juvenile fishes. The cationic polymer was substantially more toxic to lake trout fry than the anionic polymer, with 96-h LC50 estimates of 2.08 and >600 mg/l, respectively. Separate 30-d exposure experiments yielded no observed and lowest observed effect concentrations, respectively, of 0.25 and 0.5mg/l for MagnaFloc 368, and 75 and 150 mg/l for MagnaFloc 156. In both cases, behavioural responses, especially startle response, were the most sensitive test endpoints. Histopathological assessment revealed that gill pathology appeared within a few days of exposure to both polymers, apparently as a result of localized hypoxia. Acute (4 d) effects included cloudy swelling of epithelial cells, increased gill vascularization, and thickening and shortening of the gill lamella. Chronic (30 d) polymer exposure produced only slightly greater pathological effects than acute exposure, with comparable responses observed only at >1.0mg/l MagnaFloc 368 and 150 mg/l MagnaFloc 156, suggesting that the fish displayed some level of both behavioural and physiological adaptation to the respiratory stress imposed by the two polymers.
Liber
K
K
Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, Canada S7N 5B3. karsten.liber@usask.ca
Weber
L
L
Lévesque
C
C
D000180:Acrylic Resins / Q000633:toxicity*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D005427:Flocculation; D005880:Gills / Q000187:drug effects / Q000473:pathology; D007928:Lethal Dose 50; D009043:Motor Activity / Q000187:drug effects; D019055:No-Observed-Adverse-Effect Level; D011095:Polyethylenes / Q000633:toxicity*; D000644:Quaternary Ammonium Compounds / Q000633:toxicity*; D013550:Swimming; D014337:Trout; D014865:Waste Disposal, Fluid; D000099166:Polyacrylamides
D000180:Acrylic Resins; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; D000099166:Polyacrylamides; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride