Immunocharacterization of the mucin-type proteins from the intracellular stage of Trypanosoma cruzi.
The surface of Trypanosoma cruzi is covered by different groups of mucins that are differentially expressed during the parasite life cycle. We have previously identified the major mucins from the bloodstream trypomastigote stage. Here, we present additional evidence that together with our previous observations allows for the identification of a second mucin group also expressed in the mammal-dwelling stages, but predominant in the intracellular amastigote. These mucins are encoded by many genes, are mostly composed of tandem repeats and are highly conserved except for an exposed hypervariable (HV) N-terminal peptide. Antibodies against HV-peptides are restricted to approximately 50% of the chronically infected human population, are monospecific (i.e. directed towards a single HV), and display low-avidity. In contrast, immunization with a single HV-peptide triggers high-avidity, cross-reacting humoral responses against multiple HV sequences, but not against other T. cruzi surface antigens. The diversity present in the HV regions and the characteristics of the antibody response against them suggest a role of these molecules in eluding and/or modulating the mammalian host immune system.
Campo
Vanina A
VA
Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús, Universidad Nacional de General San Martín-CONICET, Av. General Paz 5445 edificio 24, San Martín (1650), Buenos Aires, Argentina. vcampo@iib.unsam.edu.ar
Buscaglia
Carlos A
CA
Di Noia
Javier M
JM
Frasch
Alberto Carlos C
AC
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D014355:Chagas Disease / Q000276:immunology; D003429:Cross Reactions; D006801:Humans; D007114:Immunization; D051379:Mice; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D014349:Trypanosoma cruzi / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism
D000913:Antibodies, Protozoan; D009077:Mucins; D010455:Peptides; D015800:Protozoan Proteins