[Agrobacterium-mediated transformation of rice mature embryos and regeneration of transgenic plants with Metr gene].
The mature embryos of a Japonica rice, Guanglingxiangnuo, were used for the study on optimization of Agrobacterium-mediated transformation. Several factors affecting the transformation were investigated and a suitable transformation system was set up. For this transformation system, the HRM medium, based on the MS medium, was suitable for inducing callus from rice mature embryos. The suitable time span of initial culture in this medium was 7-8 days before co-culturing with Agrobacterium and suitable medium for selection was CC medium. Using this transformation system, the Metr gene was introduced into Guanglingxiangnuo, and many transgenic plants were obtained. Most of these transgenic rice plants were confirmed by PCR technique and basta resistance, indicating the T-DNA had been integrated into the genome of transgenic rice plants.
Yu
Heng-Xiu
HX
Department of Agronomy, Agricultural College, Yangzhou University, Yangzhou 225009, China. hxyu@yzu.edu.cn
Liu
Qiao-Quan
QQ
Wang
Ling
L
Huang
Jian
J
Gong
Zhi-Yun
ZY
Tang
Shu-Zhu
SZ
Wang
Wei-Cheng
WC
Li
Ning
N
Gu
Ming-Hong
MH
D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D012038:Regeneration; D012231:Rhizobium / Q000235:genetics*; D012639:Seeds / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014170:Transformation, Genetic
D029681:Arabidopsis Proteins; D003470:Culture Media; D004269:DNA, Bacterial; C494590:ETR1 protein, Arabidopsis; D011956:Receptors, Cell Surface; C036483:T-DNA