title: Biophotonic hydrogen peroxide production by antibodies, T cells, and T-cell membranes.
abstract: Rapidly accumulating evidence indicates that inflammatory T cells sensitively respond to their redox environment by activating signal transduction pathways. The hypothesis that T-cell receptors have the potential to catalytically transform singlet oxygen into H(2)O(2) attracted our attention since the biophysical regulation of this process would provide a new tool for therapeutically directing T cells down a preferred signaling pathway. Light-dependent production of H(2)O(2) was first described in antibodies, and we reproduced these findings. Using a real-time H(2)O(2) sensor we extended them by showing that the reaction proceeds in a biphasic way with a short-lived phase that is fast compared to the slow second phase of the reaction. We then showed that Jurkat T cells biophotonically produce about 30nM H(2)O(2)/min/mg protein when pretreated with NaN(3). This activity was concentrated 4 to 5 times in T-cell membrane preparations. The implications of these observations for the development of new therapeutic tools for inflammatory diseases are discussed.
authors.affiliation: Cellular and Integrative Physiology, Indiana University School of Medicine, Terre Haute, IN 47809, USA. gnindl@iupui.edu
authors.forename: Gabi Nindl
authors.initials: GN
authors.lastname: Waite
authors.forename: Lee R
authors.initials: LR
authors.lastname: Waite
authors.forename: Ellen F
authors.initials: EF
authors.lastname: Hughes
authors.forename: Walter X
authors.initials: WX
authors.lastname: Balcavage
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000528:radiation effects; D002462:Cell Membrane / Q000378:metabolism* / Q000528:radiation effects*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000378:metabolism* / Q000528:radiation effects; D019169:Jurkat Cells; D008027:Light; D011829:Radiation Dosage; D013601:T-Lymphocytes / Q000378:metabolism* / Q000528:radiation effects*
chemical_list: D000906:Antibodies; D006861:Hydrogen Peroxide