[The role of lipid peroxidation products in the effect of He-Ne laser on human blood leukocytes].
The role of lipid peroxidation products formed in membranes of human blood leukocytes after irradiation with He-Ne laser was studied. It was found that low-intensity laser irradiation (0.3-1.6 J/cm2) leads to both cell activation and an increase in the content of lipid peroxidation products. The intensity of lipid peroxidation was analyzed by estimating the amount of TBA reactive products and lipid diene conjugates. Irradiation in the presence of an exogenous photosensitizer (protoporphyrin IX) enhanced the phenomena observed. The use of antioxidants (tocopherol and ionol) completely eliminated the laser-induced effects (changes in leukocyte activity and accumulation of lipid peroxidation products). These results can be explained by the fact that laser irradiation leads to the activation of lipid peroxidation in leukocyte membranes, which in turn enhances the response of cells to the stimulus (priming).
Klebanov
G I
GI
Chichuk
T V
TV
Osipov
A N
AN
Vladimirov
Iu A
IuA
D000975:Antioxidants / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D007834:Lasers; D007962:Leukocytes / Q000378:metabolism*; D008027:Light; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects*; D008054:Lipid Peroxides / Q000378:metabolism*; D017319:Photosensitizing Agents / Q000494:pharmacology; D011524:Protoporphyrins / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D024505:Tocopherols / Q000494:pharmacology
D000975:Antioxidants; D008054:Lipid Peroxides; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D017392:Thiobarbituric Acid Reactive Substances; D002084:Butylated Hydroxytoluene; C028025:protoporphyrin IX; D024505:Tocopherols