[Experimental study on the inhibitory effect of Carboxymethyl Pachymaram on hepatitis B virus expression from transfected cells].
OBJECTIVE
To observe the anti-HBV activity of Carboxymethyl Pachymaram (CMP) on the culturing of 2.2.15 cell line.


METHODS
Concentrations of 20.0 g/L, 12.0 g/L, 6.0 g/L, 3.0 g/L, 1.5 g/L of CMP were used to evaluate its toxicity to the cell line and the inhibition rates of the secretion of HBsAg and HBeAg in the cultured 2.2.15 cell line.


RESULTS
Experiments showed that the mean half toxicity concentration of CMP for 2.2.15 cell line was 13.6 g/L and concentration for 50% inhibition of the secretion of HBsAg and HBeAg were 4.45 g/L, 5.61 g/L and TI were 3.06 and 2.42. CMP showed stronger effect on anti-HBV than aciclovir.


CONCLUSIONS
CMP has good inhibitory effect on the secretion of HBsAg and HBeAg on cultured cell line 2.2.15.
Dhan
Hui-ping
HP
Department of Combination of Chinese Medicine and Western Medicine of Zhongnan Hospital, Wuhan University, Wuhan 430070, China.
Hou
An-ji
AJ
Lu
Fu-er
FE
Huang
Jiu-le
JL
D000998:Antiviral Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005936:Glucans / Q000494:pharmacology*; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D006513:Hepatitis B e Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D006801:Humans; D013997:Time Factors
D000998:Antiviral Agents; D005936:Glucans; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; C098577:carboxymethyl pachyman