title: Fenneropenaeus indicus is protected from white spot disease by oral administration of inactivated white spot syndrome virus.
abstract: Fenneropenaeus indicus could be protected from white spot disease (WSD) caused by white spot syndrome virus (WSSV) using a formalin-inactivated viral preparation (IVP) derived from WSSV-infected shrimp tissue. The lowest test quantity of lyophilized IVP coated onto feed at 0.025 g(-1) (dry weight) and administered at a rate of 0.035 g feed g(-1) body weight d(-1) for 7 consecutive days was sufficient to provide protection from WSD for a short period (10 d after cessation of IVP administration). Shrimp that survived challenges on the 5th and 10th days after cessation of IVP administration survived repeated challenges although they were sometimes positive for the presence of WSSV by a polymerase chain reaction (PCR) assay specific for WSSV. These results suggest that F. indicus can be protected from WSD by simple oral administration of IVP.
authors.affiliation: Centre for Fish Disease Diagnosis and Management, Cochin University of Science and Technology, Lakeside Campus, Kerala, India. bsingh@md3.vsnl.net.in
authors.forename: I S
authors.initials: IS
authors.lastname: Bright Singh
authors.forename: M
authors.initials: M
authors.lastname: Manjusha
authors.forename: S Somnath
authors.initials: SS
authors.lastname: Pai
authors.forename: Rosamma
authors.initials: R
authors.lastname: Philip
mesh_terms: D000821:Animal Feed / Q000821:virology; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D004267:DNA Viruses / Q000235:genetics / Q000472:pathogenicity*; D004587:Electrophoresis, Agar Gel; D033561:Penaeidae / Q000276:immunology* / Q000821:virology*; D016133:Polymerase Chain Reaction; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D015164:Vaccines, Inactivated; D014765:Viral Vaccines