DNA repair capacity measured by high throughput alkaline comet assays in EBV-transformed cell lines and peripheral blood cells from cancer patients and healthy volunteers.
We collected peripheral blood (PB) from 556 patients with various types of cancer who had undergone radiotherapy and from 81 healthy volunteers. We exposed whole PB and Epstein-Barr virus-transformed lymphoblastoid cell lines (EBLs) derived from the PB mononucleocytes to X-irradiation (5 Gy). Using the alkaline comet assay, we measured the immediate DNA damage and, at 15 min, the % residual damage. In PB, the immediate damage was similar in patients and healthy volunteers while the % residual damage (mean+/-S.D.) was significantly higher in patients with breast (54.3+/-A23.9), cervical (54.7+/-A23.9), head/neck (56.8+/-A24.4), lung (60.1+/-23.5), or esophageal cancers (59.5+/-A33.7) than in healthy donors (42.9+/-19.6) (P<0.05). We did not observe such differences in the EBV-transformed cell lines. Thus, radiation sensitivity of fresh PB cells measured by the alkaline comet assay was related to cancer status.
Iwakawa
Mayumi
M
RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences, 4-9-1, Anagawa, Inage-ku, Chiba 263-8555, Japan.
Goto
Miyako
M
Noda
Shuhei
S
Sagara
Masashi
M
Yamada
Shigeru
S
Yamamoto
Naoto
N
Kawakami
Yoshihiro
Y
Matsui
Yoshifumi
Y
Miyazawa
Yukimasa
Y
Yamazaki
Hideya
H
Tsuji
Hiroshi
H
Ohno
Tatsuya
T
Mizoe
Junetsu
J
Tsujii
Hirohiko
H
Imai
Takashi
T
D001782:Blood Donors; D002461:Cell Line, Transformed / Q000528:radiation effects; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000528:radiation effects*; D009369:Neoplasms / Q000097:blood / Q000235:genetics* / Q000532:radiotherapy; D011836:Radiation Tolerance / Q000235:genetics*