Tissue microarrays from HOPE-fixed specimens allow for enhanced high throughput molecular analyses in paraffin-embedded material.
In this study, we describe a technique that allows for the production and the use of tissue microarrays (TMA) from HOPE-fixed, paraffin-embedded specimens. The combination of these two technologies unites the advantages of the high throughput aspects in TMA, with good preservation of nucleic acids, proteins, and morphology of HOPE-fixed tissues, thus substantially widening the capabilities presently available for molecular studies in paraffin-embedded tissues.
Goldmann
Torsten
T
Research Center Borstel, Clinical and Experimental Pathology, Germany. tgoldmann@fz-borstel.de
Drömann
Daniel
D
Marzouki
Marouane
M
Schimmel
Udo
U
Debel
Karsten
K
Branscheid
Detlev
D
Zeiser
Tobias
T
Rupp
Jan
J
Gerdes
Johannes
J
Zabel
Peter
P
Vollmer
Ekkehard
E
D015415:Biomarkers; D001706:Biopsy; D005260:Female; D005404:Fixatives; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization; D008967:Molecular Biology; D016612:Paraffin Embedding; D012333:RNA, Messenger / Q000378:metabolism; D046888:Tissue Array Analysis / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods*
D015415:Biomarkers; D005404:Fixatives; D012333:RNA, Messenger