title: Successful birth after injection of frozen human oocytes with frozen epididymal spermatozoa.
abstract: A couple (female 31, male 42 years old) with infertility due to obstructive azoospermy returned to the clinic in order to attempt pregnancy using their frozen oocytes and epididymal sperm cells, which had been cryopreserved at the time of a previous IVF attempt. Two days before the scheduled transfer, eight oocytes were thawed; 5/8 (63%) oocytes survived and 4/5 (80%) oocytes fertilized after intracytoplasmic sperm injection (ICSI) with the previously frozen epididymal spermatozoa. All four fertilized ova cleaved (100%). On day 2 after thawing, four embryos were transferred; three with two cells (grade II) and one with three cells (grade III). Hormonal support for the established pregnancy was maintained with oestradiol and progesterone orally until 12 weeks of gestation, and the patient was delivered by Caesarean section at 40 weeks of gestation; the baby boy weighed 3025 g, and measured 51 cm, with Apgar of 10 in the 1st and 5th min. The cryopreservation and warming protocol used for this study yielded very favourable results, comparing well with reports in the literature. This case report demonstrates that it is possible to obtain high rates of oocyte survival following thawing and high rates of fertilization after ICSI, with viable development of the resulting embryos.
authors.affiliation: Fertilitat-Reproductive Medicine Centre, Porto Alegre, RS, Brazil. fertilitat@fertilitat.com.br
authors.forename: R
authors.initials: R
authors.lastname: Azambuja
authors.forename: M
authors.initials: M
authors.lastname: Badalotti
authors.forename: C
authors.initials: C
authors.lastname: Teloken
authors.forename: J
authors.initials: J
authors.lastname: Michelon
authors.forename: A
authors.initials: A
authors.lastname: Petracco
mesh_terms: D000328:Adult; D002470:Cell Survival; D015925:Cryopreservation; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology; D004822:Epididymis / Q000473:pathology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005615:Freezing; D006728:Hormones / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007246:Infertility / Q000628:therapy; D008297:Male; D009845:Oligospermia / Q000473:pathology; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism / Q000473:pathology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism*
chemical_list: D006728:Hormones