TFAR19 gene changes the biophysical properties of murine erythroleukemia cells.
TFAR19 is a novel apoptosis-related gene and can accelerate cell apoptosis in the presence of apoptosis inducements. Here, we studied the effects of TFAR19 on some biophysical properties of mouse erythroleukemia (MEL) cells and their molecular and structural basis. After transfected with TFAR19 and apoptosis inducement, MEL revealed a high cell membrane fluidity, a decrease in resynthesis of phospholipids, an increase in the proteins/nucleic acids ratio, a relatively orderly cytoskeleton network, an impaired deformability, a low integrin aM expression, and a decrease in adhesion to endothelial cells. These findings suggest the potential of TFAR19 for antitumor cell migration, and thus for antitumor gene therapy.
Gu
Li
L
Department of Biophysics, Health Science Center, Peking University, Beijing 100083, P. R. China.
Jiang
Yuhui
Y
Wang
Ying
Y
Yao
Weijuan
W
Sun
Dagong
D
Ka
Weibo
W
Tang
Zhiyu
Z
Xu
Xiaofeng
X
Wen
Zongyao
Z
Chien
Shu
S
D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D048430:Cell Shape; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure; D004548:Elasticity; D004730:Endothelium, Vascular / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D008560:Membrane Fluidity; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D017550:Spectroscopy, Fourier Transform Infrared; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000166:cytology; D014783:Viscosity
D051017:Apoptosis Regulatory Proteins; D015815:Cell Adhesion Molecules; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human; D010743:Phospholipids