[Species identification of biological samples by amplifying intron 8 of the TP53 gene].
OBJECTIVE
To identify the species of biological samples by amplifying intron 8 of the TP53 gene.


METHODS
Collected the bloodstains or muscle tissues of 14 kinds of common animals and human. DNA were quantified after extraction. The PCR amplification products were analyzed by PAGE and silver staining.


RESULTS
The human and monkey have one amplification band of 460 bp while the eel, fish, frog, duck, rabbit, cat, mouse, cavy, pig, ox and sheep could amplify different unspecial bands. No amplification products were found in chicken and dog.


CONCLUSIONS
The method of species identification by amplifying intron 8 of the TP53 gene is simple and sensitive.
Wang
Chuang
C
Department of Forensic Biology, College of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Zhang
Lin
L
Zhou
Bin
B
D000818:Animals; D001801:Blood Stains; D002415:Cats; D002417:Cattle; D002645:Chickens; D004247:DNA / Q000032:analysis* / Q000097:blood / Q000235:genetics; D017931:DNA Primers; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D005554:Forensic Medicine; D005784:Gene Amplification; D016158:Genes, p53; D006801:Humans; D007438:Introns / Q000235:genetics*; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D013045:Species Specificity; D013194:Staining and Labeling
D017931:DNA Primers; D004247:DNA