A death round affecting a young compartmentalized mycelium precedes aerial mycelium dismantling in confluent surface cultures of Streptomyces antibioticus.
Development-associated cell-death processes were investigated in detail during the growth and differentiation of Streptomyces antibioticus ATCC 11891 on confluent surface cultures, by using fluorescent viability probes, membrane and activity fluorescence indicators, and electron microscopy analysis. A previously unsuspected complexity was revealed, namely the presence of a very young compartmentalized mycelium that dies following an orderly pattern, leaving alternating live and dead segments in the same hypha. This death round is followed by the growth of a second mycelium which develops rapidly from the live segments of the first mycelium and dies massively in a second death round, which extends over the phases of aerial mycelium formation and sporulation.
Manteca
Ángel
Á
Area de Microbiología, Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Julián Clavería s/n, 33006 Oviedo, Spain.
Fernández
Marisol
M
Laboratorio de Proteómica, Centro Nacional de Biotecnología, Cantoblanco, 28049 Madrid, Spain.
Sánchez
Jesús
J
Area de Microbiología, Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Julián Clavería s/n, 33006 Oviedo, Spain.
D002463:Cell Membrane Permeability; D003470:Culture Media; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D025282:Mycelium / Q000254:growth & development* / Q000648:ultrastructure; D009930:Organic Chemicals; D011419:Propidium; D013303:Streptomyces antibioticus / Q000254:growth & development* / Q000648:ultrastructure
D003470:Culture Media; D009930:Organic Chemicals; C103389:SYTO 9; D011419:Propidium