Effect of denaturation on the photochemistry of pyrimidine bases in isolated DNA.
The influence of denaturation on DNA photochemistry was studied by quantifying the yield of formation of all possible bipyrimidine photolesions within isolated genomic DNA samples exposed to UVC radiation. Effects of DNA melting was studied either by carrying out irradiation over a wide range of temperature (0-90 degrees C) or by decreasing the ionic strength of the solution at 30 degrees C. A first observation was a much larger decrease in the photoreactivity upon increasing the temperature in single-stranded than in double-stranded DNA. Secondly, formation of trans,syn cyclobutane dimers and, to a lesser extent, modification in the ratio between the yields of cyclobutane dimers and (6-4) photoproducts, were found to be other main features associated with denaturation. These results emphasize the modulating role of structure in the yield and nature of UV-induced DNA damage.
Douki
Thierry
T
Laboratoire Lésions des Acides Nucléiques, Service de Chimie Inorganique et Biologique UMR-E 3 CEA-UJF, CEA/DSM/Département de Recherche Fondamentale sur la Matière Condensée, CEA-Grenoble, 38054 Grenoble Cedex 9, France. tdouki@cea.fr
D003503:Cyclobutanes / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000378:metabolism; D009994:Osmolar Concentration; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
D003503:Cyclobutanes; D004277:DNA, Single-Stranded; D011740:Pyrimidine Dimers; D004247:DNA