Novel way of capping mRNA trimer and studies of its interaction with human nuclear cap-binding complex.
Binding of mRNA 5' cap by the nuclear cap-binding complex (CBC) is crucial for a wide variety of mRNA metabolic events. The interaction involving the CBP20 subunit of CBC is mediated by numerous hydrogen bonds and by stacking of the tyrosine sidechains with two first bases of the capped mRNA. To examine a possible role of a longer mRNA chain in the CBC-cap recognition, we have synthesized an mRNA tetramer using a novel way of capping an RNA trimer and determined its affinity for CBC by fluorescence titration.
Worch
Remigiusz
R
Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warsaw, Poland.
Stepinski
Janusz
J
Niedzwiecka
Anna
A
Jankowska-Anyszka
Marzena
M
Mazza
Catherine
C
Cusack
Stephen
S
Stolarski
Ryszard
R
Darzynkiewicz
Edward
E
D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D008956:Models, Chemical; D039502:Nuclear Cap-Binding Protein Complex / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012315:RNA Caps / Q000737:chemistry*; D012322:RNA Precursors; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
D046911:Macromolecular Substances; D039502:Nuclear Cap-Binding Protein Complex; D012315:RNA Caps; D012322:RNA Precursors; D012333:RNA, Messenger; D012261:Ribonucleoproteins