[Abnormalities of microsatellite in transitional cell carcinoma of urinary bladder related with aromatic amine exposure].
OBJECTIVE
To study the microsatellite abnormalities of the aromatic amine exposure-associated transitional cell carcinoma (TCC) and sporadic TCC of urinary bladder, and to evaluate the potential of microsatellite analysis on detection of this diseases.


METHODS
Based on our previous investigations, 5 microsatellite markers (D17S695, D9S162, D3S1295, DBH and D3S1234) that had high frequencies of loss of heterozygosity (LOH) in sporadic TCC, were selected for analysis with the bladder lesions derived from 16 patients with aromatic amine exposure history. The microsatellite analysis with urine sediments from the post-operated patients was also carried out.


RESULTS
There was at least one informative marker out of the 5 microsatellite foci showed polymorphism in the DNA derived from 16 patients examined. Within 87.50% (14/16) patients, LOH was detected in the bladder lesions at least with one microsatellite marker. The LOH frequency of D3S1295 was higher in occupational TCC patients than that in sporadic TCC patients. The diagnostic accordance rate of patients showed LOH in at least one microsatellite marker with patients diagnosed by pathology was 81.25% (13/16). In the urine sediments from 8 TCC post-operated patients, LOH was found at least with one microsatellite marker.


CONCLUSIONS
There could be a different LOH pattern in aromatic amine exposure-associated TCC, and genes near D3S1295 might play a role in the occupational exposure-associated TCC.
Di
Xue-bing
XB
Department of Etiology and Carcinogenesis, Cancer Institute, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021, China.
Zheng
Shan
S
Chen
Wen
W
Li
Jian-lin
JL
Liu
Jian-zhong
JZ
Zhang
Peng
P
Li
Ai-qin
AQ
An
Zhi-yuan
ZY
Feng
Pei-wen
PW
Gao
Yan-ning
YN
D002295:Carcinoma, Transitional Cell / Q000235:genetics / Q000473:pathology*; D006801:Humans; D006841:Hydrocarbons, Aromatic / Q000633:toxicity*; D018895:Microsatellite Repeats; D016273:Occupational Exposure; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000473:pathology*
D006841:Hydrocarbons, Aromatic