title: An assessment of the effects of two types of bioresorbable barriers to prevent postoperative intra-abdominal adhesions in rats.
abstract: OBJECTIVE
  This study assessed and compared the efficacy of two types of bioresorbable membranes in the prevention of postoperative adhesion under clean contaminated and bacterial peritonitis conditions using a cecal ligation and puncture model in rats.
  
  
  METHODS
  Wistar albino rats (n = 72) were divided into six groups. Bacterial peritonitis was induced using a cecal ligation and puncture model in groups 2, 4, and 6. Groups 1, 3, and 5 served as controls for clean contaminated procedures in the absence of bacterial peritonitis. Groups 1 and 2 were the untreated clean contaminated and bacterial peritonitis groups and served as controls for the effect of the bioresorbable membranes in each condition. In groups 3 and 4, a 1.5 x 3 cm USP glycerol/sodium hyaluronate/carboxymethylcellulose membrane was wrapped around the cecal resection area and a 2 x 4 cm membrane was left under the incision. The oxidized regenerated cellulose membrane was similarly applied in groups 5 and 6. Four weeks later, the adhesions were evaluated. In addition, fibrosis and inflammation were observed histopathologically.
  
  
  RESULTS
  Adhesion development (P = .008), fibrosis (P = .008), and inflammation (P = .0001) differed among the groups. Both materials increased adhesion formation in the bacterial peritonitis condition. Increased fibrotic activity was detected in all material-applied groups under both conditions. In addition, more inflammation was detected in the groups that received the application of a material, especially in the presence of bacterial peritonitis.
  
  
  CONCLUSIONS
  Neither material prevented adhesions in clean contaminated conditions. Moreover, they increased adhesion formation in bacterial peritonitis.
authors.affiliation: Department of General Surgery, Gaziosmanpasa University Medical School, Tokat, Turkey.
authors.forename: Huseyin Ayhan
authors.initials: HA
authors.lastname: Kayaoglu
authors.forename: Namik
authors.initials: N
authors.lastname: Ozkan
authors.forename: Selcuk Mevlut
authors.initials: SM
authors.lastname: Hazinedaroglu
authors.forename: Omer Faik
authors.initials: OF
authors.lastname: Ersoy
authors.forename: Resit Dogan
authors.initials: RD
authors.lastname: Koseoglu
mesh_terms: D000005:Abdomen; D000818:Animals; D001424:Bacterial Infections / Q000503:physiopathology / Q000601:surgery; D001672:Biocompatible Materials; D002480:Cellulase; D002483:Cellulose, Oxidized; D005355:Fibrosis; D005990:Glycerol; D006820:Hyaluronic Acid; D007249:Inflammation; D007813:Laparotomy / Q000009:adverse effects*; D008297:Male; D008567:Membranes, Artificial; D010538:Peritonitis / Q000503:physiopathology / Q000601:surgery; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000209:etiology / Q000473:pathology / Q000517:prevention & control
chemical_list: D001672:Biocompatible Materials; D002483:Cellulose, Oxidized; D008567:Membranes, Artificial; C118198:Seprafilm; D006820:Hyaluronic Acid; D002480:Cellulase; C019826:carboxymethylcellulase; D005990:Glycerol