Role of gastric mucosal ascorbic acid in gastric mucosal lesion development in rats with water immersion restraint stress.
We examined the role of gastric mucosal ascorbic acid (AA) in gastric mucosal lesion development in rats with water immersion restraint stress (WIRS). When fasted rats were subjected to WIRS for 1, 3 or 6 h, gastric mucosal lesions developed at 3 and 6 h. Gastric mucosal AA concentration decreased at 3 and 6 h after the onset of WIRS, while gastric mucosal non-protein SH concentration decreased at 1, 3, and 6 h and gastric mucosal vitamin E concentration decreased at 6 h. Gastric mucosal lipid peroxide concentration and myeloperoxidase activity increased at 3 and 6 h of WIRS. Pre-administration of AA (250 mg/kg) prevented gastric mucosal development with attenuation of the decreased gastric mucosal AA, non-protein SH and vitamin E concentrations, and the increased gastric mucosal lipid peroxide concentration and myeloperoxidase activity. These results suggest that gastric mucosal AA plays an important role in WIRS-induced gastric mucosal lesion development.
Ohta
Yoshiji
Y
Department of Chemistry, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan. yohta@fujita-hu.ac.jp
Kamiya
Yoshio
Y
Imai
Yoichiro
Y
Arisawa
Tomiyasu
T
Nakano
Hiroshi
H
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism / Q000502:physiology*; D005753:Gastric Mucosa / Q000737:chemistry* / Q000293:injuries; D007101:Immersion / Q000009:adverse effects; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical / Q000009:adverse effects / Q000379:methods; D013272:Stomach Diseases / Q000209:etiology / Q000378:metabolism; D013312:Stress, Physiological / Q000209:etiology / Q000503:physiopathology; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013997:Time Factors; D014810:Vitamin E / Q000378:metabolism; D014867:Water
D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; D014867:Water; D014810:Vitamin E; D009195:Peroxidase; D001205:Ascorbic Acid