title: [Screening of the genes of hepatitis B virus e antigen interacting proteins].
abstract: OBJECTIVE
  To screen and clone the genes in hepatocytes which encode protein that can interact with hepatitis B e antigen(HBeAg) by yeast-two hybridization.
  
  
  METHODS
  Recombined HBeAg bait plasmid (pGBKT7-eAg) was transformed into yeast AH l09, followed by mating with yeast Yl87 containing liver cDNA library plasmid in 2 x YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-Ade-His) which contains X-a-gal for selecting positive blue clones. Then positive clones were selected and plasmids were prepared and sequenced. Finally, bioinformatics analysis was performed.
  
  
  RESULTS
  Totally 245 positive colonies were selected and 101 colonies were sequenced. Through sequences alignment, 6 novel genes and 35 recorded genes were screened.
  
  
  CONCLUSIONS
  Genes of HBeAg interacting proteins have been cloned successfully, which brings some new clues for further studies on the biological functions of HBeAg and the related proteins.
authors.affiliation: Department of Immunology, Institute of Infectious Disease of The No. 302 Hospital, Beijing 100039, China.
authors.forename: Bo-an
authors.initials: BA
authors.lastname: Li
authors.forename: Cui-li
authors.initials: CL
authors.lastname: Shu
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Yang
authors.initials: Y
authors.lastname: Qi
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Wei-ping
authors.initials: WP
authors.lastname: He
authors.forename: Yun
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D015723:Gene Library; D006513:Hepatitis B e Antigens / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques
chemical_list: D006513:Hepatitis B e Antigens