title: Diversity of denitrifying bacteria isolated from Daejeon Sewage Treatment Plant.
abstract: The diversity of the denitrifying bacterial populations in Daejeon Sewage Treatment Plant was examined using a culture-dependent approach. Of the three hundred and seventy six bacterial colonies selected randomly from agar plates, thirty-nine strains that showed denitrifying activity were selected and subjected to further analysis. According to the morphological and biochemical properties, the thirty nine isolates were divided into seven groups. This grouping was supported by an unweighted pair group method, using an arithmetic mean (UPGMA) analysis with fatty acid profiles. Restriction pattern analysis of 16S rDNA with four endonucleases (AluI, BstUI, MspI and RsaI) again revealed seven distinct groups, consistent with those defined from the morphological and biochemical properties and fatty acid profiles. Through the phylogenetic analysis using the 16S rDNA partial sequences, the main denitrifying microbial populations were found to be members of the phylum, Proteobacteria; in particular, classes Gamma proteobacteria (Aeromonas, Klebsiella and Enterobacter) and Beta proteobacteria (Acidovorax, Burkholderia and Comamonas), with Firmicutes, represented by Bacillus, also comprised a major group.
authors.affiliation: Department of Wood Science, University of British Columbia, 2900-2424 Main Mall, Vancouver, B.C. V6T 1Z4, Canada.
authors.forename: Young-Woon
authors.initials: YW
authors.lastname: Lim
authors.forename: Soon-Ae
authors.initials: SA
authors.lastname: Lee
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Kim
authors.forename: Hae-Young
authors.initials: HY
authors.lastname: Yong
authors.forename: Seon-Hee
authors.initials: SH
authors.lastname: Yeon
authors.forename: Yong-Keun
authors.initials: YK
authors.lastname: Park
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Jeong
authors.forename: Jin-Sook
authors.initials: JS
authors.lastname: Park
mesh_terms: D015373:Bacterial Typing Techniques; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D014644:Genetic Variation; D009584:Nitrogen / Q000378:metabolism*; D020560:Proteobacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D009584:Nitrogen