title: Engineering DNA topology with locked nucleosides: a structural study.
abstract: DNA dodecamers modified with nucleotide building blocks based on a bicyclo[3. 1.0]hexane system that effectively locks the ribose template into an RNA-like or North (N) conformation were analyzed by various biophysical techniques including high field nuclear magnetic resonance (NMR). Replacement of either one or both of the center thymidines in the Dickerson Drew dodecamer (CGCGAAT*T*CGCG) caused a progressive shift in the bending propensity of the double helix as shown by a newly developed rapid technique that compares the residual dipolar coupling (RDC) values of the modified duplexes with those previously determined for the native DNA.
authors.affiliation: Laboratory of Medicinal Chemistry, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Maderia
authors.forename: Justin
authors.initials: J
authors.lastname: Wu
authors.forename: Ad
authors.initials: A
authors.lastname: Bax
authors.forename: Shilpa
authors.initials: S
authors.lastname: Shenoy
authors.forename: Barry
authors.initials: B
authors.lastname: O'Keefe
authors.forename: Victor E
authors.initials: VE
authors.lastname: Marquez
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Barchi
mesh_terms: D001703:Biophysics / Q000379:methods; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes; D009705:Nucleosides / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry; D009838:Oligodeoxyribonucleotides; D013696:Temperature; D013816:Thermodynamics
chemical_list: D009692:Nucleic Acid Heteroduplexes; D009705:Nucleosides; D009711:Nucleotides; D009838:Oligodeoxyribonucleotides; D004247:DNA