title: Dietary inulin intake and age can significantly affect intestinal absorption of calcium and magnesium in rats: a stable isotope approach.
abstract: BACKGROUND
  previous studies have shown that non-digestible inulin-type fructan intake can increase intestinal mineral absorption in both humans and animals. However, this stimulatory effect on intestinal absorption may depend on experimental conditions such as duration of fermentable fiber intake, mineral diet levels and animals' physiological status, in particular their age.
  
  
  OBJECTIVE
  the aim of this study was to determine the effect of inulin intake on Ca and Mg absorption in rats at different age stages.
  
  
  METHODS
  eighty male Wistar rats of four different ages (2, 5, 10 and 20 months) were randomized into either a control group or a group receiving 3.75% inulin in their diet for 4 days and then 7.5% inulin for three weeks. The animals were fed fresh food and water ad libitum for the duration of the experiment. Intestinal absorption of Ca and Mg was determined by fecal monitoring using stable isotopic tracers. Ca and Mg status was also assessed.
  
  
  RESULTS
  absorption of Ca and Mg was significantly lower in the aged rats (10 and 20 mo) than in the young and adult rat groups. As expected, inulin intake increased Ca and Mg absorption in all four rat groups. However, inulin had a numerically greater effect on Ca absorption in aged rats than in younger rats whereas its effect on Mg absorption remained similar across all four rat age groups.
  
  
  CONCLUSIONS
  the extent of the stimulatory effect of inulin on absorption of Ca may differ according to animal ages. Further studies are required to explore this effect over longer inulin intake periods, and to confirm these results in humans.
authors.affiliation: Centre de Recherche en Nutrition Humaine d'Auvergne, Unité Maladies Métaboliques et Micro-nutriments, INRA, Theix, 63122 St-Genès-Champanelle, France. coudray@ensam.inra.fr
authors.forename: Charles
authors.initials: C
authors.lastname: Coudray
authors.forename: Mathieu
authors.initials: M
authors.lastname: Rambeau
authors.forename: Christine
authors.initials: C
authors.lastname: Feillet-Coudray
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Tressol
authors.forename: Christian
authors.initials: C
authors.lastname: Demigne
authors.forename: Elyett
authors.initials: E
authors.lastname: Gueux
authors.forename: Andrzej
authors.initials: A
authors.lastname: Mazur
authors.forename: Yves
authors.initials: Y
authors.lastname: Rayssiguier
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000097:blood; D002127:Calcium Isotopes; D002136:Calcium, Dietary / Q000493:pharmacokinetics*; D002432:Cecum / Q000378:metabolism; D004032:Diet; D004435:Eating; D005243:Feces / Q000737:chemistry; D005285:Fermentation; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology*; D007444:Inulin / Q000008:administration & dosage*; D007554:Isotopes; D008274:Magnesium / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D009752:Nutritional Status; D051381:Rats; D017208:Rats, Wistar; D015430:Weight Gain
chemical_list: D002127:Calcium Isotopes; D002136:Calcium, Dietary; D007554:Isotopes; D007444:Inulin; D008274:Magnesium; D002118:Calcium