Ultrasound-microbubble-mediated NF-kappaB decoy transfection attenuates neointimal formation after arterial injury in mice.
OBJECTIVE
Decoy transfection is a significant methodology for suppressing gene activation. The decoy can be synthesized easily; however, the major obstacle is the difficulty involved in effective in vivo delivery.


RESULTS
We used a fluorescein-labeled decoy to investigate the ultrasound-microbubble-mediated in vivo delivery in normal and injured mouse arteries. We showed that this approach could deliver the decoy into target tissues. In addition, we performed in vivo NF-kappaB decoy transfection into murine injured arteries using the ultrasound-microbubble method. Murine femoral arteries were injured using flexible wires to establish arterial injury. Pathologically, neointima/media areas in the NF-kappaB decoy transfection using ultrasound-microbubble group showed less than those in the control groups. Immunohistochemistry revealed that enhanced expression of inflammatory factors was observed in nontreated injured arteries, while the NF-kappaB decoy suppressed the expression.


CONCLUSIONS
We revealed that ultrasound-microbubble delivery of the decoy is effective for transfection into target organs. We also indicated that NF-kappaB decoy transfection using this method has potential for the suppression of neointimal formation. Ultrasound-mediated transfection of the decoy can be beneficial for the clinical treatment of restenosis after coronary intervention and other cardiovascular diseases.
Inagaki
Hiroshi
H
Department of Cardiovascular Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Suzuki
Jun-ichi
J
Ogawa
Masahito
M
Taniyama
Yoshiaki
Y
Morishita
Ryuichi
R
Isobe
Mitsuaki
M
D000818:Animals; D005263:Femoral Artery / Q000276:immunology / Q000293:injuries* / Q000473:pathology; D015316:Genetic Therapy / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D045423:Microbubbles; D016328:NF-kappa B / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D017539:Tunica Intima / Q000276:immunology / Q000473:pathology; D014465:Ultrasonics; D014657:Vasculitis / Q000276:immunology / Q000473:pathology
D016328:NF-kappa B