The hairy and enhancer of split 1 is a negative regulator of the repressor element silencer transcription factor.
Silencing of the transcriptional repressor REST is required for terminal differentiation of neuronal and beta-cells. In this study, we hypothesized that REST expression is controlled by hairy and enhancer of split 1 (HES-1), a transcriptional repressor that plays an important role in brain and pancreas development. We identified several N elements (CTNGTG) within the promoter of REST and confirmed that HES-1 associates with the endogenous promoter of REST. Moreover, using a cells model that overexpress HES-1 and a combination of experimental approaches, we demonstrated that HES-1 reduces endogenous REST expression. Taken together, these results indicate that HES-1 is an upstream negative regulator of REST expression.
Abderrahmani
Amar
A
Department of Internal Medicine, University of Lausanne, 1005 Lausanne, Switzerland. amar.abderrahmani@unil.ch
Niederhauser
Guy
G
Lenain
Vincent
V
Regazzi
Romano
R
Waeber
Gérard
G
D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006367:HeLa Cells; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020218:Response Elements / Q000235:genetics*; D000072056:Transcription Factor HES-1; D014157:Transcription Factors / Q000235:genetics*; D000099229:RE1-Silencing Transcription Factor
D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D012097:Repressor Proteins; D000072056:Transcription Factor HES-1; D014157:Transcription Factors; D000099229:RE1-Silencing Transcription Factor; C078493:HES1 protein, human