title: SNARE-mediated membrane traffic modulates RhoA-regulated focal adhesion formation.
abstract: In the present study, we examined the role of soluble NSF attachment protein receptor (SNARE)-mediated membrane traffic in the formation of focal adhesions during cell spreading. CHO-K1 cells expressing a dominant-negative form of N-ethylmaleimide-sensitive factor (E329Q-NSF) were unable to spread as well as control cells and they formed focal adhesions (FAs) that were larger than those in control cells. FA formation was impaired in cells transfected with a dominant-negative form of RhoA, but, significantly, not in cells simultaneously expressing dominant-negative NSF. Treatment of E329Q-NSF-expressing cells with the ROCK inhibitor Y-27632 did inhibit FA formation. The results are consistent with a model of cell adhesion in which SNARE-mediated membrane traffic is required for both the elaboration of lamellipodia and the modulation of biochemical signals that control RhoA-mediated FA assembly.
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ont., Canada N1G 2W1.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Gonon
authors.forename: Michael
authors.initials: M
authors.lastname: Skalski
authors.forename: Michelle
authors.initials: M
authors.lastname: Kean
authors.forename: Marc G
authors.initials: MG
authors.lastname: Coppolino
mesh_terms: D000577:Amides / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D006224:Cricetinae; D003412:Cricetulus; D022001:Focal Adhesions / Q000187:drug effects; D047908:Intracellular Signaling Peptides and Proteins; D052066:N-Ethylmaleimide-Sensitive Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D021381:Protein Transport; D011725:Pyridines / Q000494:pharmacology; D050600:SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D022502:Stress Fibers / Q000378:metabolism; D013744:Tetanus Toxin / Q000235:genetics; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism; D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: D000577:Amides; D047908:Intracellular Signaling Peptides and Proteins; D011725:Pyridines; D050600:SNARE Proteins; D013744:Tetanus Toxin; C108830:Y 27632; D017346:Protein Serine-Threonine Kinases; D054460:rho-Associated Kinases; D052066:N-Ethylmaleimide-Sensitive Proteins; D020830:rac1 GTP-Binding Protein; D020742:rhoA GTP-Binding Protein