[HPLC-electrospray ionization ion trap tandem mass spectrometry analysis of oxymatrine and its metabolites in rat urine].
OBJECTIVE
To identify the main metabolites of oxymatrine (OMT) in rats.


METHODS
To optimize the conditions of LC/ESI-ITMS' chromatograms and spectra by oxymatrine and matrine (MT), and summarize their ionization and cleavage rules in ESIMS, then serving as the basis for the metabolite analyses of oxymatrine in rats. To collect the 0-24 h urine samples of the rats after ip 40 mg x kg(-1) oxymatrine, the samples were enriched and purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by LC/ESI-ITMS. The structures of OMT metabolites were identified according to their retention times and ESI-ITMSn rules.


RESULTS
Six phase I metabolites and the parent drug OMT were found in the rat urine, and the main metabolite was MT. No phase II metabolites were found.


CONCLUSIONS
The developed LC/ESI-ITMSn methods to identify the metabolites of oxymatrine in rats is not only simple and rapid but also sensitive and specific. This technology is one of the most efficient methods for the analysis of drug metabolites.
Chen
Yong
Y
Hubei Province Key Lab of Bio-Technology of Traditional Chinese Medicine, Hubei University, Wuhan 430062, China. cy101610@npc.gov.cn
Chen
Huai-xia
HX
Du
Peng
P
Han
Feng-mei
FM
D000470:Alkaloids / Q000302:isolation & purification / Q000493:pharmacokinetics* / Q000652:urine*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D010946:Plants, Medicinal / Q000737:chemistry; D011807:Quinolizines / Q000302:isolation & purification / Q000493:pharmacokinetics* / Q000652:urine*; D051381:Rats; D017208:Rats, Wistar; D029909:Sophora / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; C037573:oxymatrine; D000093842:Matrines