Tissue-engineered hybrid tooth and bone.
Tooth loss accompanied by alveolar bone resorption presents a significant clinical problem. We have investigated the utility of a tissue-engineering approach to provide corrective therapies for tooth-bone loss. Hybrid tooth-bone tissues were bioengineered as follows. Tooth implants were generated from pig third molar tooth bud cells seeded onto polyglycolide (PGA) and polyglycolide-colactide (PLGA) scaffolds, and grown for 4 weeks in the omenta of adult rat hosts. Bone implants were generated from osteoblasts induced from bone marrow progenitor cells obtained from the same pig, seeded onto PLGA fused wafer scaffolds, and grown for 10 days in a rotational oxygen-permeable bioreactor system. The tooth and bone implants were harvested, sutured together, reimplanted, and grown in the omenta for an additional 8 weeks. Histological and immunohistochemical analyses of the excised hybrid tooth-bone constructs revealed the presence of tooth tissues, including primary and reparative dentin and enamel in the tooth portion of hybrid tooth-bone implants, and osteocalcin and bone sialoprotein-positive bone in the bone portion of hybrid tooth-bone constructs. Collagen type III-positive connective tissue resembling periodontal ligament and tooth root structures were present at the interface of bioengineered tooth and bone tissues. These results demonstrate the utility of a hybrid tooth-bone tissue-engineering approach for the eventual clinical treatment of tooth loss accompanied by alveolar bone resorption.
Young
Conan S
CS
Department of Cytokine Biology, Forsyth Institute, Boston, Massachusetts 02115, USA.
Abukawa
Harutsugi
H
Asrican
Rose
R
Ravens
Michael
M
Troulis
Maria J
MJ
Kaban
Leonard B
LB
Vacanti
Joseph P
JP
Yelick
Pamela C
PC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry; D019149:Bioreactors; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D024061:Collagen Type III / Q000378:metabolism; D003238:Connective Tissue / Q000378:metabolism; D006651:Histocytochemistry; D006652:Histological Techniques; D007085:Ilium / Q000166:cytology; D007150:Immunohistochemistry; D019344:Lactic Acid / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D051379:Mice; D008819:Mice, Nude; D008954:Models, Biological; D008963:Molar / Q000166:cytology / Q000254:growth & development / Q000637:transplantation; D009805:Odontogenesis / Q000502:physiology*; D009852:Omentum / Q000601:surgery; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D012795:Sialoglycoproteins / Q000378:metabolism; D013552:Swine; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D014070:Tooth / Q000166:cytology / Q000254:growth & development* / Q000637:transplantation; D014083:Tooth Germ / Q000166:cytology / Q000254:growth & development* / Q000637:transplantation; D014183:Transplantation, Heterologous; D016042:Transplantation, Heterotopic
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D024061:Collagen Type III; D011108:Polymers; D012795:Sialoglycoproteins; D015675:Osteocalcin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid