Comparison of histologic, biochemical, and mechanical properties of murine skin treated with the 1064-nm and 1320-nm Nd:YAG lasers.
The goal of this study was to compare the effects of the Q-switched 1064-nm Nd:YAG laser and the 1320-nm Nd:YAG laser non-ablative treatments on mouse skin in vivo. Skin elasticity measurements were carried out with a Reviscometer, and skin samples were taken for histological study, hydroxyproline content assay and estimation of collagen type I and III. By the second month after non-ablative treatments, the 1064-nm laser treatment resulted in an average of 25% greater improvement of skin elasticity, 6% more increase of dermal thickness, and 11% higher synthesis of hydroxyproline than the 1320-nm laser. Collagen type III increased markedly after the 1064-nm laser treatment whereas more collagen type I was elicited by the 1320-nm laser. Our results demonstrated that the 1064-nm laser was more effective than the 1320-nm Nd:YAG laser in non-ablative treatments, but the results needed to be confirmed in humans. It appeared that photo-mechanic reaction could cause more collagen type III synthesis whereas the photo-thermal effect was in favor of the formation of collagen type I.
Dang
Yong-Yan
YY
Institute for Laser Medicine & Biophotonics, Shanghai Jiaotong University, Shanghai, China.
Ren
Qiu-Shi
QS
Liu
Hua-Xu
HX
Ma
Jing-Bo
JB
Zhang
Jin-Sheng
JS
D000818:Animals; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D004548:Elasticity; D006909:Hydroxyproline / Q000378:metabolism; D007834:Lasers; D051379:Mice; D008815:Mice, Inbred Strains; D012867:Skin / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000528:radiation effects*
D024042:Collagen Type I; D024061:Collagen Type III; D006909:Hydroxyproline