[Effect of urotensin II on secretion of adrenomedullin from human vascular endothelial cells].
OBJECTIVE
To study the effect of human urotensin II (HU II) on secretion of adrenomedullin (ADM) from human vascular endothelial cells (HVEC) and its mechanism.


METHODS
In cultured HVEC, different concentrations of HUII were used to stimulate the ADM secretion from HVEC, and the inhibitors of different signal transduction pathway were used to investigate their effects on ADM secretion. The contents of ADM in medium were determined by radio immunoassay.


RESULTS
HUII stimulated secretion of ADM from HVEC in a time-dependent and concentration-dependent manner. The contents of ADM in the experiment groups were changed compared with that in control group (P < 0.05). The increase of ADM could be inhibited by inhibitor of extracellular signal-regulated protein kinase (PD(98059)), inhibitor of P38 kinase (SB(202190)), inhibitor of calmodulin (W(7)) and inhibitor of Ca(2+) (nicardipine) (P < 0.05). The inhibition ratio in those groups was 68%, 78%, 24% and 25% respectively. But the inhibitor of Calcineurin (CaN) and inhibitor of protein kinase C (H(7)) had no influence on the secretion of ADM from HVEC (P > 0.05).


CONCLUSIONS
The stimulated effect of HUII on the ADM secretion from HVEC may be mediated by Ca(2+), ERKs, CaM-PK and P38 signal transduction pathways.
Shi
Xiang-dong
XD
Department of Cardiology, Zhujiang Hospital, South Medical University, Guangzhou 510282, China.
Li
Zhi-liang
ZL
Wu
Hong-chao
HC
Lü
Yong-heng
YH
Wang
Tong-han
TH
Fu
Qiang
Q
Xu
Chun-sheng
CS
Tang
Chao-shu
CS
D053607:Adrenomedullin / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D015398:Signal Transduction / Q000187:drug effects*; D014579:Urotensins / Q000494:pharmacology*
D014579:Urotensins; D053607:Adrenomedullin; C029677:urotensin II; D002118:Calcium