Effects of the number of actin-bound S1 and axial force on X-ray patterns of intact skeletal muscle.
Effects of the number of actin-bound S1 and of axial tension on x-ray patterns from tetanized, intact skeletal muscle fibers were investigated. The muscle relaxant, BDM, reduced tetanic M3 meridional x-ray reflection intensity (I(M3)), M3 spacing (d(M3)), and the equatorial I(11)/I(10) ratio in a manner consistent with a reduction in the fraction of S1 bound to actin rather than by generation of low-force S1-actin isomers. At complete force suppression, I(M3) was 78% of its relaxed value. BDM distorted dynamic I(M3) responses to sinusoidal length oscillations in a manner consistent with an increased cross-bridge contribution to total sarcomere compliance, rather than a changed S1 lever orientation in BDM. When the number of actin-bound S1 was varied by altering myofilament overlap, tetanic I(M3) at low overlap was similar to that in high [BDM] (79% of relaxed I(M3)). Tetanic d(M3) dependence on active tension in overlap experiments differed from that observed with BDM. At high BDM, tetanic d(M3) approached its relaxed value (14.34 nm), whereas tetanic d(M3) at low overlap was 14.50 nm, close to its value at full overlap (14.56 nm). This difference in tetanic d(M3) behavior was explicable by a nonlinear thick filament compliance which is extended by both active and passive tension.
Griffiths
P J
PJ
University Laboratory of Physiology, Oxford, United Kingdom.
Bagni
M A
MA
Colombini
B
B
Amenitsch
H
H
Bernstorff
S
S
Funari
S
S
Ashley
C C
CC
Cecchi
G
G
D008841:Actin Cytoskeleton / Q000737:chemistry; D000199:Actins / Q000737:chemistry*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D009119:Muscle Contraction; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D015879:Myosin Subfragments / Q000378:metabolism; D009218:Myosins; D016011:Normal Distribution; D009991:Oscillometry; D020033:Protein Isoforms; D011896:Rana temporaria; D012518:Sarcomeres / Q000378:metabolism; D012542:Scattering, Radiation; D013314:Stress, Mechanical; D013997:Time Factors; D014961:X-Ray Diffraction; D014965:X-Rays
D000199:Actins; D015879:Myosin Subfragments; D020033:Protein Isoforms; D009218:Myosins