title: Rapid action of pesticides on cytosolic calcium concentrations in cultures of human umbilical vein endothelial cells.
abstract: Persistent metabolites of pesticides such as p,p'-DDE, at environmentally relevant concentrations, have been shown to have a rapid effect on intracellular calcium [Ca2+]i concentrations in human granulosa-lutein cells. Since endocrine disrupting substances can be transferred from the maternal circulation to the fetus the present study examined whether the pesticides, kepone, o,p-DDE, p,p'-DDE and methoxychlor, could alter cytoplasmic calcium [Ca2+]cyt concentrations in human umbilical vein endothelial (HUVE) cells. Cultured HUVE cells were loaded with Fura-2 AM and changes in [Ca2+]cyt of single cells were studied using a dynamic digital Ca2+ imaging system. Kepone and methoxychlor consistently increased [Ca2+]cyt concentrations, similar to the effects of estradiol and progesterone. p,p'-DDE increased [Ca2+]cyt concentrations in 80% of experiments whereas o,p-DDE stimulated its increases in 42%. Estrone, estriol, pregnenolone and cortisol were not effective. These results demonstrate that pesticides can have a rapid effect on HUVE cells probably through a nongenomic mechanism of action.
authors.affiliation: Department of Obstetrics & Gynecology, Reproductive Biology Division, McMaster University Medical Centre, 1200 Main Street West, Health Sciences Centre, Hamilton, Ont., Canada L8N 3Z5. younglai@mcmaster.ca
authors.forename: Edward V
authors.initials: EV
authors.lastname: Younglai
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Wu
authors.forename: Warren G
authors.initials: WG
authors.lastname: Foster
mesh_terms: D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002478:Cells, Cultured; D002614:Chelating Agents; D007631:Chlordecone / Q000633:toxicity; D003600:Cytosol / Q000378:metabolism; D003633:Dichlorodiphenyl Dichloroethylene / Q000633:toxicity; D004533:Egtazic Acid; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D000077267:Fulvestrant; D006801:Humans; D037342:Pertussis Toxin / Q000494:pharmacology; D010575:Pesticides / Q000633:toxicity*; D011374:Progesterone / Q000494:pharmacology; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism; D019284:Thapsigargin / Q000494:pharmacology; D013997:Time Factors; D014471:Umbilical Veins
chemical_list: D002614:Chelating Agents; D004791:Enzyme Inhibitors; D004965:Estrogen Antagonists; D010575:Pesticides; D000077267:Fulvestrant; D011374:Progesterone; D003633:Dichlorodiphenyl Dichloroethylene; D004958:Estradiol; D004533:Egtazic Acid; D019284:Thapsigargin; D037342:Pertussis Toxin; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D007631:Chlordecone; D002118:Calcium