[The virogenic status of cultured continuous simian lymphoid cells and their immunophenotypical characteristics].
Immunophenotyping of cultured continuous simian lymphoid cultures using a panel containing monoclonal antibodies to human B- and T-cell antigens has been carried out, by employing enzyme immunoassay and flow cytofluorometry. The test cultures showed a wide variety of cells containing B- and T-cell antigens with their varying expression. The cultures were also found to comprise lymphoblasts simultaneously containing B- and T-cell markers. Simian lymphotropic viruses, such as EBV-like and STLV-1 retrovirus, detected apart or simultaneously, have been verified by polymerase chain reaction. Whether there is a possible relationship between the type of cells in the culture and the type of their replicating virus(es) is discussed in the paper.
Agrba
V Z
VZ
Lapin
B A
BA
Medvedeva
N M
NM
Chikobava
M G
MG
Iakovleva
L A
LA
Karal-ogly
D D
DD
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005814:Genes, Viral / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D016130:Immunophenotyping; D008223:Lymphoma / Q000276:immunology* / Q000821:virology*; D016133:Polymerase Chain Reaction; D011323:Primates; D015303:Simian T-lymphotropic virus 1 / Q000235:genetics / Q000302:isolation & purification*
D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte