[Effect of Ganoderma triterpene on proliferation of dendritic cells from mouse spleen].
OBJECTIVE
To investigate the effect of Ganoderma Triterpene (GT) on proliferation of Dendritic cells (DC) from mouse spleen.


METHODS
Compared with the cytokine (GM-CSF + IL-4), we investigated the effect of GT of different concentrations and cytokine + GT of different concentrations on proliferation of DC from mouse spleen by MTT.


RESULTS
GT (40-200 microg/ml) could stimulate the proliferation of DC significantly, but the stimulations weren't obvious compared with the cytokine. GT + cytokine, compared with the negative control, had obviously promoting effect and was better than the cytokine. The results above indicated GT could not only stimulate the proliferation of DC but also significantly coorderate with the cytokine.


CONCLUSIONS
GT may play the role by its growth factor-like function, and by cooperating with the cytokine in the regulation of DC.
Wang
Bin
B
Institute of Basic Medical sciences, Guangzhou UTCM, Guangzhou 510405.
Hu
Yueshan
Y
Li
Jiefen
J
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects*; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005260:Female; D032902:Ganoderma / Q000737:chemistry*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D014315:Triterpenes / Q000494:pharmacology*
D016207:Cytokines; D004365:Drugs, Chinese Herbal; D014315:Triterpenes; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor