Tubulin nitration in human gliomas.
Immunohistochemical and biochemical investigations showed that significant protein nitration occurs in human gliomas, especially in grade IV glioblastomas at the level of astrocytes and oligodendrocytes and neurones. Enhanced alpha-tubulin immunoreactivity was co-present in the same elements in the glioblastomas. Proteomic methodologies were employed to identify a nitrated protein band at 55 kDa as alpha-tubulin. Peptide mass fingerprinting procedures demonstrated that tubulin is nitrated at Tyr224 in grade IV tumour samples but is unmodified in grade I samples and in non-cancerous brain tissue. These results provide the first characterisation of endogenously nitrated tubulin from human tumour samples.
Fiore
Gabriella
G
Zoological Station Anton Dohrn, Laboratory of Biochemistry and Molecular Biology, Villa Comunale, I-80121 Naples, Italy.
Di Cristo
Carlo
C
Monti
Gianluca
G
Amoresano
Angela
A
Columbano
Laura
L
Pucci
Pietro
P
Cioffi
Fernando A
FA
Di Cosmo
Anna
A
Palumbo
Anna
A
d'Ischia
Marco
M
D000368:Aged; D001253:Astrocytes / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods; D001932:Brain Neoplasms / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014404:Tubulin / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D014404:Tubulin; C002744:3-nitrotyrosine; D014443:Tyrosine