A simple method for stem cell labeling with fluorine 18.
Hexadecyl-4-[(18)F]fluorobenzoate ([(18)F]HFB), a long chain fluorinated benzoic acid ester, was prepared in a one-step synthesis by aromatic nucleophilic substitution of [(18)F]fluoride ion on hexadecyl-4-(N,N,N-trimethylammonio)benzoate. The radiolabeled ester was obtained in good yields (52% decay corrected) and high purity (97%). [(18)F]HFB was used to radiolabel rat mesenchymal stem cells (MSCs) by absorption into cell membranes. MicroPET imaging of [(18)F]HFB-labeled MSCs following intravenous injection into the rat showed the expected high and persistent accumulation of radioactivity in the lungs. [(18)F]HFB is thus simple to prepare and uses labeling agent for short-term distribution studies of injected stem cells.
Ma
Bing
B
Department of Radiology, Division of Nuclear Medicine, University of Michigan Medical School, Ann Arbor, 48109, USA.
Hankenson
Kurt D
KD
Dennis
James E
JE
Caplan
Arnold I
AI
Goldstein
Steven A
SA
Kilbourn
Michael R
MR
D000818:Animals; D002452:Cell Count / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D005462:Fluorine Radioisotopes / Q000493:pharmacokinetics*; D008168:Lung / Q000166:cytology / Q000000981:diagnostic imaging* / Q000378:metabolism*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000000981:diagnostic imaging* / Q000378:metabolism*; D049268:Positron-Emission Tomography / Q000379:methods*; D051381:Rats; D011916:Rats, Inbred F344
D005462:Fluorine Radioisotopes