On-probe sample pretreatment for direct analysis of lipids in gram-positive bacterial cells by matrix-assisted laser desorption ionization mass spectrometry.
On-probe sample pretreatment using trifluoroacetic acid as an additional reagent enabled the direct detection of phospholipids in whole bacteria by means of matrix-assisted laser desorption ionization mass spectrometry for not only gram-negative organisms but also gram-positive ones with a thicker peptidoglycan layer.
Ishida
Yasuyuki
Y
Division of Energy Science, EcoTopia Science Institute, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. ishida@esi.nagoya-u.ac.jp
Kitagawa
Kuniyuki
K
Nakayama
Akihito
A
Ohtani
Hajime
H
D001412:Bacillus subtilis / Q000737:chemistry / Q000166:cytology; D001431:Bacteriological Techniques; D006094:Gram-Positive Bacteria / Q000737:chemistry* / Q000166:cytology; D010743:Phospholipids / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014269:Trifluoroacetic Acid / Q000494:pharmacology
D010743:Phospholipids; D014269:Trifluoroacetic Acid