Oxidation of biotin during oligonucleotide synthesis.
A new "polystyrene biotin support" has been synthesized for the solid support synthesis of the 3'-biotinylated oligonucleotides. Several oligos were synthesized and were analyzed by the HPLC and Mass Spec. Oligo analysis revealed that the biotin gets oxidized to "biotin sulfoxide" during the synthesis.
Upadhya
Krishna
K
Applied Biosystems, 8a50 Lincoln Centre Drive, Foster City, CA 94404, USA. upadhykg@appliedbiosystems.com
Khattak
Iqbal K
IK
Mullah
Bashar
B
D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry*; D019921:Biotinylation; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D001710:Biotin; C017072:D-biotin-d-sulfoxide; D010100:Oxygen