[Inhibitory effects of chloride channel blockers NPPB on proliferation of human glomerular mesangial cells].
OBJECTIVE
To investigate the effects of NPPB, a chloride channel blocker, on proliferation of mesangial cells.


METHODS
Cell proliferation was determined by measuring cell number and 3H-thymidine incorporation. The LDH activity released from these cells was measured as evaluation of cell viability. The phase of cell cycle was detected by flow cytometry.


RESULTS
Cell proliferation assays showed that treatment with both NPPB (50 and 25 micromol x L(-1)) and in hypertonic media (100% increased osmolarity with D-mannitol ) significantly reduced the number of human MC and 3H-thymidine incorporation in a dose-dependent manner. But the LDH activity was not significantly altered in the treatment with 50 micromol x L(-1) NPPB. Flow cytometry experiments showed that 50 and 25 micromol x L(-1) NPPB arrested (84.2 +/- 2.4) % and (80.8 +/- 2.9) % of cells at G0/G1 stage, versus (70.5 +/- 1.4) % of control cells. Conclusion NPPB suppresses cell proliferation and produces growth arrest at G0/G1 phase in human MC by a mechanism probably associated with changes in cell volume.
Jiao
Jun-dong
JD
Department of Pharmacology, Harbin Medical University, Bio-pharmaceutical Key Laboratory of Heilongjiang Province, China.
Yue
Peng
P
Du
Zhi-min
ZM
Dong
De-li
DL
Ai
Jing
J
Yang
Bao-feng
BF
D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D018118:Chloride Channels / Q000037:antagonists & inhibitors*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D050527:Mesangial Cells / Q000166:cytology* / Q000378:metabolism; D009579:Nitrobenzoates / Q000008:administration & dosage / Q000494:pharmacology*
D018118:Chloride Channels; D009579:Nitrobenzoates; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D007770:L-Lactate Dehydrogenase