title: [The effect of HGF on graft-versus-host disease and graft-versus-leukemia after allogeneic bone marrow transplantation in acute lymphoblastic leukemia mice].
abstract: OBJECTIVE
  To investigate the effect of hepatocyte growth factor (HGF) on graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) after allogeneic bone marrow transplantation (allo-BMT) and related mechanism in acute lymphoblastic leukemia (ALL) mice.
  
  
  METHODS
  Twenty nude mice were randomly divided into control (group A) and test (group B) groups for monitoring relapse, and 20 BALB/c mice into control (group C) and test (group D) groups for GVHD. HGF as injected from day 0 to day 7 after BMT for groups B and D, while PBS for A and C. CD4(+) and CD8(+) T cell were evaluated by flow cytometry. The survival of mice after BMT was recorded. The level of tumor necrosis factor-alpha (TNF-alpha) was evaluated by ELISA.
  
  
  RESULTS
  The median past-BMT survival were 7.00 +/- 1.58, 9.00 +/- 1.58, 11.00 +/- 3.95 and 24.00 +/- 13.44 days for groups A, B, C, D, respectively, being prolonged in group D. HGF could decrease the quantity of CD4(+) T cells [group D (10.39 +/- 1.15)% vs group C (13.50 +/- 1.80)%, P < 0.01] and increase CD8(+) T cell [group D (12.25 +/- 2.85)% vs group C (6.12 +/- 1.99)%, P < 0.01], decrease the level of TNF-alpha in transplanted ALL mice [group D (112.10 +/- 18.99) pg/ml vs group C (143.90 +/- 25.35) pg/ml, P < 0.01] and reduce the degree of GVHD.
  
  
  CONCLUSIONS
  HGF could alleviate post-allo-BMT GVHD but retain GVL effect.
authors.affiliation: People's Hospital, Wuhan University, Wuhan 430060, China.
authors.forename: Yun-jin
authors.initials: YJ
authors.lastname: Xia
authors.forename: Qing-ping
authors.initials: QP
authors.lastname: Gao
authors.forename: Chu-cheng
authors.initials: CC
authors.lastname: Wan
authors.forename: Fan-jun
authors.initials: FJ
authors.lastname: Cheng
authors.forename: Zhi-xiang
authors.initials: ZX
authors.lastname: Liu
authors.forename: Ren-ci
authors.initials: RC
authors.lastname: Guo
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D004195:Disease Models, Animal; D005260:Female; D006086:Graft vs Host Disease / Q000517:prevention & control*; D020544:Graft vs Leukemia Effect / Q000187:drug effects*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000601:surgery; D011897:Random Allocation; D014184:Transplantation, Homologous
chemical_list: D017228:Hepatocyte Growth Factor