Efficient detection of PrPSc (263K) in human plasma.
The potential contamination of human blood or plasma with prions, such as variant Creuftzfelt-Jacob disease (vCJD), is becoming a serious problem. In this study, we established a Western blot-based detection method for PrP(Sc) (263K) spiked in plasma. Although plasma contains a large amount of protein, specific detection of a small amount of 263K in plasma could be specifically detected only after ultra-centrifugation followed by heat-denaturation of plasma proteins included in the resulting precipitate, before the digestion with proteinase K.
Fujita
Ryo
R
Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Urayama
Takeru
T
Ohmizu
Akimasa
A
Yunoki
Mikihiro
M
Tsuji
Shoutaro
S
Ikuta
Kazuyoshi
K
D015153:Blotting, Western / Q000379:methods*; D007562:Creutzfeldt-Jakob Syndrome / Q000175:diagnosis*; D006801:Humans; D018620:PrPSc Proteins / Q000097:blood*; D011489:Protein Denaturation; D014461:Ultracentrifugation
D018620:PrPSc Proteins