title: [Therapeutic neovascularization with autologous bone marrow CD34+ cells transplantation in hindlimb ischemia].
abstract: OBJECTIVE
  To explore whether transplantation of autologous bone marrow stem cells might augment angiogenesis and collateral vessel formation in a canine model of hindlimb ischemia.
  
  
  METHODS
  CD34(+) stem cells were centrifugation through Ficoll and an immune magnetic cell sorting system from bone marrow (20 ml) of canine (n = 5) and induced into endothelial cells with VEGF in vitro, and expression of von Willebrand factor. Bilateral hindlimb ischemia was surgically induced in canines and Dil fluorescence labeled autologous stem cells were transplanted into the ischemic tissues.
  
  
  RESULTS
  Four weeks after transplantation, fluorescence microscopy revealed that transplanted cells were incorporated into the capillary network among preserved skeletal myocytes. The stem cells transplanted group had more angiographically detectable collateral vessels, a higher capillary density (12.0 +/- 2.8 vs. 5.0 +/- 1.6 per field; t = 4.17 P < 0.05) and a higher ABI (0.58 +/- 0.14 vs. 0.32 +/- 0.11; t = 2.95, P < 0.05).
  
  
  CONCLUSIONS
  Direct local transplantation of autologous bone marrow CD34(+) stem cells seems to be a useful strategy for therapeutic neovascularization in ischemic tissues in adults, consistent with "therapeutic vasculogenesis."
authors.affiliation: Department of Vascular Surgery, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. doczhk@yahoo.com.cn
authors.forename: Hong-kun
authors.initials: HK
authors.lastname: Zhang
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Feng
authors.forename: Hai-ge
authors.initials: HG
authors.lastname: Zhao
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Chen
authors.forename: Chun-hu
authors.initials: CH
authors.lastname: Yang
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D002454:Cell Differentiation; D004195:Disease Models, Animal; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D006614:Hindlimb / Q000098:blood supply*; D007511:Ischemia / Q000628:therapy*; D008297:Male; D018919:Neovascularization, Physiologic
chemical_list: D018952:Antigens, CD34