Identification of a rice gene (Bph 1) conferring resistance to brown planthopper (Nilaparvata lugens Stal) using STS markers.
This study was carried out to identify a high-resolution marker for a gene conferring resistance to brown planthopper (BPH) biotype 1, using japonica type resistant lines. Bulked segregant analyses were conducted using 520 RAPD primers to identify RAPD fragments linked to the BPH resistance gene. Eleven RAPDs were shown to be polymorphic amplicons between resistant and susceptible progeny. One of these primers, OPE 18, which amplified a 923 bp band tightly linked to resistance, was converted into a sequence-tagged-site (STS) marker. The STS marker, BpE18-3, was easily detectable as a dominant band with tight linkage (3.9cM) to Bph1. It promises to be useful as a marker for assisted selection of resistant progeny in backcross breeding programs to introgress the resistance gene into elite japonica cultivars.
Kim
Suk-Man
SM
Department of Agronomy, College of Agriculture, Kyungpook National University, Daegu 702-701, Korea.
Sohn
Jae-Keun
JK
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D005819:Genetic Markers / Q000235:genetics*; D006430:Hemiptera / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000469:parasitology; D010935:Plant Diseases / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods / Q000592:standards; D012641:Selection, Genetic; D016415:Sequence Alignment; D016324:Sequence Tagged Sites
D005819:Genetic Markers