title: [Molecular diversity of the gene encoding Fe(II)-oxidizing enzyme in Acidithiobacillus ferrooxidans].
abstract: Acidithiobacillus ferrooxidans (A.f) is a Gram-negative chemolithotrophic bacterium able to oxidize ferrous iron, elemental sulfur and inorganic sulfur compounds. Fe(II)-oxidizing enzyme plays key role in Fe(II)-oxidizing system. Different A.f strains, which were isolated and purified from various ecological niches, show obviously phenotypic diversity after comparison rates of growth and oxidation of Fe2+. The Fe(II)-oxidizing enzyme gene(iro) for five strains was identified by PCR reactions uitilising primers and genomic DNA, then sequenced and multialignment. The results show there is a putative high variable region in CDS between the 187th and 195th bp and the sequence similarities are from 97% to 99%. For strain YTW, P187-189 Thr-->Pro; For P193-195, Met-->Asn for strain TK; Met-->Ile for strain BY; For P219, all strains T-->C. For upstream of CDS, sequences of all strains are identical to the Genebank sequence (E03451), but downstream of CDS, the sequences exist some variable sites too.
authors.affiliation: School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Peng
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.forename: Jie-Wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Hu
mesh_terms: D042763:Acidithiobacillus / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D014644:Genetic Variation; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D018744:DNA, Plant; D007501:Iron; D010088:Oxidoreductases; C074859:iron oxidase, Thiobacillus ferrooxidans