Prostaglandin D2 synthase and its post-translational modifications in neurological disorders.
Prostaglandin D2 synthase (PGDS) (beta-trace protein) is a highly abundant cerebrospinal fluid (CSF) glycoprotein. A number of studies have been performed to determine the potential value of this protein for the diagnosis of various neurological disorders. The measurement of total PGDS levels in CSF has proved marginally useful for this purpose, but promising results were obtained while investigating changes in the posttranslational modifications (PTM) pattern. Using 2-DE analysis, we previously showed that PGDS is differentially expressed in ante- and post mortem CSF samples. In the present study, we examined whether the PGDS isoforms may help to distinguish stroke and neurodegenerative disease patients from healthy subjects. The pattern of PGDS PTM was analyzed in CSF from patients with various neurological disorders (n = 44) using IEF/immunoblotting techniques. Strong alterations of this pattern were detected in patients with different forms of degenerative dementia. These findings are consistent with PGDS being altered in some neurological diseases and provide new opportunities for clinical applications.
Lescuyer
Pierre
P
Biomedical Proteomics Research Group, Department of Bioinformatics and Structural Biology, Geneva University, Geneva, Switzerland. Pierre.Lescuyer@medecine.unige.ch
Gandini
Alberto
A
Burkhard
Pierre R
PR
Hochstrasser
Denis F
DF
Sanchez
Jean-Charles
JC
D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000134:cerebrospinal fluid; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000134:cerebrospinal fluid* / Q000378:metabolism; D054834:Lipocalins; D008297:Male; D008875:Middle Aged; D019636:Neurodegenerative Diseases / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D020033:Protein Isoforms / Q000134:cerebrospinal fluid; D011499:Protein Processing, Post-Translational; D020521:Stroke / Q000134:cerebrospinal fluid* / Q000175:diagnosis
D015415:Biomarkers; D054834:Lipocalins; D020033:Protein Isoforms; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase