title: Gain-of-function screen for genes that affect Drosophila muscle pattern formation.
abstract: This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.
authors.affiliation: Max Planck Institut für biophysikalische Chemie, Göttingen, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Staudt
authors.forename: Andreas
authors.initials: A
authors.lastname: Molitor
authors.forename: Kalman
authors.initials: K
authors.lastname: Somogyi
authors.forename: Juan
authors.initials: J
authors.lastname: Mata
authors.forename: Silvia
authors.initials: S
authors.lastname: Curado
authors.forename: Karsten
authors.initials: K
authors.lastname: Eulenberg
authors.forename: Martin
authors.initials: M
authors.lastname: Meise
authors.forename: Thomas
authors.initials: T
authors.lastname: Siegmund
authors.forename: Thomas
authors.initials: T
authors.lastname: Häder
authors.forename: Andres
authors.initials: A
authors.lastname: Hilfiker
authors.forename: Günter
authors.initials: G
authors.lastname: Brönner
authors.forename: Anne
authors.initials: A
authors.lastname: Ephrussi
authors.forename: Pernille
authors.initials: P
authors.lastname: Rørth
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Cohen
authors.forename: Sonja
authors.initials: S
authors.lastname: Fellert
authors.forename: Ho-Ryun
authors.initials: HR
authors.lastname: Chung
authors.forename: Olaf
authors.initials: O
authors.lastname: Piepenburg
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schäfer
authors.forename: Herbert
authors.initials: H
authors.lastname: Jäckle
authors.forename: Gerd
authors.initials: G
authors.lastname: Vorbrüggen
mesh_terms: D000818:Animals; D019521:Body Patterning; D002453:Cell Cycle; D002465:Cell Movement; D003599:Cytoskeleton / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D005821:Genetic Techniques; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D025801:Ubiquitin