title: The human placental derived BeWo cell line: a useful model for selecting Plasmodium falciparum CSA-binding parasites.
abstract: Chondroitin sulfate A (CSA) present in the placental intervillous blood spaces has been described as the main receptor involved in the massive sequestration of Plasmodium falciparum parasitized erythrocytes to the placenta. Placental parasite isolates are functionally distinct from isolates that sequester in other organs, because they do not cytoadhere to CD36 but instead bind to CSA. To investigate for the parasites molecules associated with the CSA adhesion phenotype, different methodologies have been developed to select for CSA-binding lines in vitro mainly using non-placental sources of CSA that differ in their sulfation pattern. In this study, we show that the human trophoblastic BeWo cell line is a very efficient alternative to select for the CSA-binding phenotype in parasitized erythrocytes.
authors.affiliation: Unité de Biologie des Interactions Hôte-Parasite, CNRS URA 2581, Institut Pasteur, 25 rue du Dr Roux, F-75724 Paris Cedex 15, France.
authors.forename: Nicola K
authors.initials: NK
authors.lastname: Viebig
authors.forename: Marta C
authors.initials: MC
authors.lastname: Nunes
authors.forename: Artur
authors.initials: A
authors.lastname: Scherf
authors.forename: Benoit
authors.initials: B
authors.lastname: Gamain
mesh_terms: D000818:Animals; D018955:CD36 Antigens / Q000378:metabolism; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002809:Chondroitin Sulfates / Q000378:metabolism*; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism / Q000469:parasitology; D005260:Female; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology; D010641:Phenotype; D010920:Placenta / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000469:parasitology
chemical_list: D018955:CD36 Antigens; D002809:Chondroitin Sulfates