The common marmoset monkey as a model for implantation and early pregnancy research.
This chapter describes methods used to investigate implantation in the common marmoset monkey, Callithrix jacchus. A reverse-transcriptase polymerase chain reaction-strategy with which to detect transcripts for steroid receptors and enzymes involved in estradiol biosynthesis is described, and an immunohistochemistry approach for detecting proteins within the implantation site is presented.
Einspanier
Almuth
A
Institute of Physiological Chemistry, Facility of Veterinary Medicine, University of Leipzig, Germany.
Lieder
Kai
K
Einspanier
Ralf
R
Husen
Bettina
B
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D002144:Callithrix / Q000502:physiology*; D010064:Embryo Implantation / Q000502:physiology*; D004958:Estradiol / Q000096:biosynthesis; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D023421:Models, Animal; D011247:Pregnancy / Q000502:physiology*; D011374:Progesterone / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012099:Reproductive Techniques; D014599:Uterus / Q000166:cytology / Q000378:metabolism
D047628:Estrogen Receptor alpha; D011374:Progesterone; D004958:Estradiol; D012313:RNA; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; D001141:Aromatase