[PCR based identification of strains of endophytic ecotype of Bacillus amyloliquefaciens].
A search for genetic markers of an endophytic ecotype of Bacillus amyloliquefaciens was performed by the subtractive hybridization technique. The isolated fragments specificity was checked by DNA-DNA hybridization. The sequence of a DNA locus exhibiting the highest taxon specificity has manifested a 76% identity to the gene mtlA from Bacillus subtilis 168 genome. A set of discriminative primers has been derived from this sequence which could be used for the fast and reliable identification of isolates of the endophytic ecotype of B. amyloliquefaciens placing them separate against their close relatives including the type cultures of B. amyloliquefaciens and B. subtilis. Identification of strains of this ecotype is quite important as far as their characteristic ability to colonize the roots and internal tissues of plants in association with high antagonistic activity in respect of many phytopathogenic fungi and bacteria makes them valuable agents for plant protection.
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D001407:Bacillus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002876:Chromosomes, Bacterial / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D005819:Genetic Markers; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004269:DNA, Bacterial; D005819:Genetic Markers