[The simultaneous determination of laetrile, paeoniflorin and paeonol in Jingzhi Guizhi Fuling capsule by HPLC].
OBJECTIVE
To establish an HPLC method for the simultaneous determination of three major bioactive components in Jingzhi Guizhi Fuling capsules namely laetrile, paeoniflorin and paeonol.


METHODS
A LiChrospher C18 column (4.6 mm x 250 mm, 5 microm) was used. The chromatography was carried out with a stepwise gradient programming. The mobile phase was acetonitrile-water (containing 0.1% phosphorous acid) and the flow rate was 1.0 mL x min.


RESULTS
The linear range of laetrile was 12.87-102.94 micron x mL(-1), r = 0.999 9, paeoniflorin 24.84 - 198.7 microg x mL(-1), r = 0.9999 and paeonol 12.57-100.56 microg x mL(-1), r = 0.999 9. The method is accurate with variation less than 1.5 % and recovery more than 95 %.


CONCLUSIONS
The method was successfully applied to analyze three major bioactive components in Jingzhi Guizhi Fuling capsules.
Liao
Zheng-gen
ZG
China Pharmaceutical University, Nanjing 210009, China.
Ling
Ya
Y
Zhong
Yan
Y
Ping
Qi-neng
QN
D000098:Acetophenones / Q000032:analysis*; D000678:Amygdalin / Q000032:analysis*; D001565:Benzoates / Q000032:analysis*; D001952:Bridged-Ring Compounds / Q000032:analysis*; D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D027424:Cinnamomum / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D005960:Glucosides / Q000032:analysis*; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D020072:Polyporales / Q000737:chemistry; D015203:Reproducibility of Results; D006027:Glycosides
D000098:Acetophenones; D000678:Amygdalin; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides; C013638:paeonol