title: Glutaraldehyde-induced occupational asthma: BALF components and BALF and serum Clara cell protein (CC16) changes due to specific inhalatory provocation test.
abstract: OBJECTIVE
  The purpose of this study was to evaluate bronchoalveolar lavage fluid (BALF) components and Clara cell protein (CC16) concentration in serum and BALF in patients with glutaraldehyde (GA)-induced asthma, before and after a specific inhalatory provocation test (SIPT) with GA, in comparison to atopic asthmatics and healthy individuals.
  
  
  METHODS
  Spirometry and bronchoalveolar lavage were performed before and after SIPT. The serum and BALF concentrations of CC16 and cytogram content in BALF were evaluated.
  
  
  RESULTS
  In GA-sensitized asthmatics, the level of CC16 in BALF and serum was significantly lower at 24 h after SIPT in comparison with the values recorded prior to the experiment. There was a significant increase in the proportion of eosinophils, basophils and lymphocytes in BALF of GA-sensitized asthmatics obtained after SIPT.
  
  
  CONCLUSIONS
  The determination of CC16 either in serum or in BALF is a non-invasive test to detect Clara cell damage.
authors.affiliation: Department of Occupational Diseases, Centre of Occupational and Environmental Allergy, Nofer Institute of Occupational Medicine, 8 Teresy St, 90-950 Lodz, Poland.
authors.forename: Cezary
authors.initials: C
authors.lastname: Palczynski
authors.forename: Jolanta
authors.initials: J
authors.lastname: Walusiak
authors.forename: Anna
authors.initials: A
authors.lastname: Krakowiak
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Halatek
authors.forename: Urszula
authors.initials: U
authors.lastname: Ruta
authors.forename: Patrycja
authors.initials: P
authors.lastname: Krawczyk-Adamus
authors.forename: Tomasz
authors.initials: T
authors.lastname: Wittczak
authors.forename: Radoslaw
authors.initials: R
authors.lastname: Swiercz
authors.forename: Pawel
authors.initials: P
authors.lastname: Gorski
authors.forename: Konrad
authors.initials: K
authors.lastname: Rydzynski
mesh_terms: D000328:Adult; D001249:Asthma / Q000139:chemically induced* / Q000175:diagnosis / Q000276:immunology; D001491:Basophils; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000276:immunology; D016022:Case-Control Studies; D019742:Chemokines, CC / Q000032:analysis* / Q000097:blood; D004804:Eosinophils; D005260:Female; D005976:Glutaral / Q000633:toxicity*; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis / Q000209:etiology / Q000276:immunology; D007958:Leukocyte Count; D008168:Lung / Q000503:physiopathology; D008214:Lymphocytes; D009784:Occupational Diseases / Q000139:chemically induced* / Q000175:diagnosis / Q000276:immunology; D013147:Spirometry
chemical_list: D015415:Biomarkers; D019742:Chemokines, CC; C070959:Ccl6 protein, mouse; D005976:Glutaral