Nanodissection of single- and double-stranded DNA by atomic force microscopy.
Nanodissection of single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) has been investigated by atomic force microscopy (AFM). It is found that both ss- and dsDNA can be repeatedly dissected by an AFM tip. However, a comparison study indicates that ssDNA is a little bit more easily broken by the AFM tip than dsDNA. This is supported by the fact that the time requested to break ssDNA is shorter than that of dsDNA in the same dissection procedure under the same load. Our experiment also shows that dissection of the DNA strand is very sensitive to the load applied, and a small change of the load lead to different results.
An
Hong-jie
HJ
College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Guo
Yun-chang
YC
Zhang
Xiao-dong
XD
Zhang
Yi
Y
Hu
Jun
J
D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure*; D008422:Materials Testing / Q000379:methods; D008832:Microchemistry / Q000379:methods*; D042282:Microdissection / Q000379:methods*; D008846:Micromanipulation / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D010812:Physical Stimulation / Q000379:methods; D013314:Stress, Mechanical
D004247:DNA