Presence of 2',5'-linkages in a homopyrimidine DNA oligonucleotide promotes stable triplex formation under physiological conditions.
We prepared 15-mer homopyrimidine oligonucleotides containing three or four 2',5'-linked DNA units, and their ability as a triplex-forming oligonucleotide (TFO) was analyzed in detail UV melting experiments showed that replacement of a 3',5'-linkage by a 2',5'-linkage at every third or fourth residue in TFO significantly promoted stable triplex formation under physiological conditions.
Obika
Satoshi
S
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
Hiroto
Akiko
A
Nakagawa
Osamu
O
Imanishi
Takeshi
T
D001482:Base Composition; D020029:Base Pairing; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry*; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D009696:Nucleic Acids / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry*; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
D017931:DNA Primers; D009692:Nucleic Acid Heteroduplexes; D009696:Nucleic Acids; D009841:Oligonucleotides; D011743:Pyrimidines; C051802:triplex DNA; D004247:DNA