Enrichment, cultivation, and detection of reductively dechlorinating bacteria.
Strategies and procedures for enriching, isolating, and cultivating reductively dechlorinating bacteria that use chloroorganic compounds as metabolic electron acceptors from environmental samples are described. Further, nucleic acid-based approaches used to detect and quantify dechlorinator (i.e., Dehalococcoides)-specific genes are presented.
Löffler
Frank E
FE
School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta 30332-0512, USA.
Sanford
Robert A
RA
Ritalahti
Kirsti M
KM
D000085:Acetates / Q000378:metabolism; D001421:Bacteria, Anaerobic / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D006867:Hydrolases / Q000235:genetics; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012989:Soil Pollutants / Q000378:metabolism
D000085:Acetates; D003470:Culture Media; D004269:DNA, Bacterial; D006843:Hydrocarbons, Chlorinated; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D006859:Hydrogen; D006867:Hydrolases; C034507:2-haloacid dehalogenase