title: Preparation and characterization of SARS in-house reference antiserum.
abstract: A reference antiserum for SARS is in urgent need in the development of SARS vaccine and other serological test of SARS research. Convalescent serum was collected from clinical confirmed patient. ELISA, Western-blotting and neutralization assay detected specific antibody against SARS coronavirus. This antiserum was prepared as in-house reference antiserum, freeze-dried and sealed in ampoules. The potency of this reference antiserum is defined to be 52.7 U after extensive calibration. Further, collaborative studies for the evaluation of this serum are needed in order to satisfy the requirements for international reference antiserum.
authors.affiliation: Sinovac Biotech Co. Ltd., No. 39, Shangdi Xi Road, Haidian District, Beijing 100085, PR China. ZhangJS@Sinovac.com
authors.forename: Jiansan
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Liangxiang
authors.initials: L
authors.lastname: Hu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gao
authors.forename: Zhenshan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
authors.forename: Jiangting
authors.initials: J
authors.lastname: Chen
authors.forename: Xuejie
authors.initials: X
authors.lastname: Gong
authors.forename: Lifei
authors.initials: L
authors.lastname: Song
authors.forename: Yufen
authors.initials: Y
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Shufen
authors.initials: S
authors.lastname: Li
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Huang
authors.forename: Ye
authors.initials: Y
authors.lastname: Ning
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Dong
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wei
authors.forename: Guanmu
authors.initials: G
authors.lastname: Dong
authors.forename: Weidong
authors.initials: W
authors.lastname: Yin
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002522:Chlorocebus aethiops; D003289:Convalescence; D006801:Humans; D009500:Neutralization Tests; D012015:Reference Standards; D045473:Severe acute respiratory syndrome-related coronavirus / Q000276:immunology* / Q000502:physiology; D012698:Serologic Tests / Q000592:standards; D045169:Severe Acute Respiratory Syndrome / Q000097:blood / Q000276:immunology*; D014709:Vero Cells
chemical_list: D000914:Antibodies, Viral