[The expression of TNF-alpha in the intestine of mice irradiated by neutron and gamma rays].
OBJECTIVE
To study the expression of tumor necrosis factor alpha (TNF-alpha) in the intestine of mice irradiated by neutron and gamma rays.


METHODS
350 male BALB/c mice were irradiated with neutron and gamma rays of different doses, and sacrificed at 6 and 12 hours, 1, 2, 3, 4, 5, 7, 10, 14, 21 and 28 days after irradiation. The TNF-alpha in the mice intestinal tissue was detected by means of immunohistochemical staining and image analysis.


RESULTS
In normal control mice, TNF-alpha was expressed in the cytoplasm of macrophages in intestinal villus interstitium, submucosa and lymph tissue. After 2.5Gy neutron radiation, TNF-alpha was decreased progressively within 2 days, increased obviously in macrophages and crypt cells during the 3rd-7th day, reached the peak at the 5th day and recovered to normal level at the 14th day. TNF-alpha was decreased progressively within 4 days after 4.0 and 5.5Gy neutron and 12Gy gamma ray irradiation. TNF-gamma was increased obviously in 6-12 hours, decreased on the first day, increased at the 2nd-5th day, peaked at the third day and recovered at the 10th day after 5.5Gy gamma ray irradiation.


CONCLUSIONS
Neutron and gamma ray radiation induce different expression profile of endogenous TNF-gamma in small intestine, which may be related with the pathologic courses of irradiation-induced damage and repair of intestine.
Peng
Rui-yun
RY
Beijing Institute of Radiation Medicine, Beijing 100850, China. pengry@nic.bmi.ac.cn
Gao
Ya-bing
YB
Chen
Hao-yu
HY
Fu
Kai-fei
KF
Ma
Jun-jie
JJ
Wang
Xiao-min
XM
Hu
Wen-hua
WH
Wang
De-wen
DW
D000818:Animals; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009502:Neutrons; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D014409:Tumor Necrosis Factor-alpha