title: Intestinal gene expression in TNBS treated mice using genechip and subtractive cDNA analysis: implications for Crohn's disease.
abstract: So far it has proven difficult to identify a causative gene(s) or gene product initiating the events that lead to inflammation of the intestinal mucosa and, ultimately, progression to Crohn's disease (CD), an inflammatory bowel disease. However, gene transcripts identified in the intestine of trinitrobenzene sulfonic acid (TNBS)-treated mice might suggest a clue, and even represent candidate genes leading to inflammation and mucosal damage, and to subsequent fibrosis. In the present study, DNA microarray (13000 transcripts) methodology was applied to mucosal RNA extracted from TNBS-treated mice, some transcripts of which were validated via cDNA subtraction and RT-PCR analyses. Intestinal biopsy samples from CD patients were then analyzed using cDNA mini-array (1300 cDNAs), focusing on gene transcripts associated with cancer and immunity. Mini-array results revealed transcript changes similar and also dissimilar to those found from the DNA microarray analysis. These changes, previously known or newly identified, possibly occurring during the initial and progressive stages of inflammatory conditions may provide a clue to identify marker transcripts and/or targets for the development of future gene therapy.
authors.affiliation: Laboratory of Animal Breeding, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Kazuto
authors.initials: K
authors.lastname: Isuzugawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Murase
authors.forename: Masami
authors.initials: M
authors.lastname: Iwata
authors.forename: Tomiyasu
authors.initials: T
authors.lastname: Arisawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakano
authors.forename: Norihiro
authors.initials: N
authors.lastname: Nishimura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yamato
authors.forename: Michio
authors.initials: M
authors.lastname: Ohta
authors.forename: Kenji
authors.initials: K
authors.lastname: Ina
authors.forename: Takahisa
authors.initials: T
authors.lastname: Murata
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Hori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ozaki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Imakawa
mesh_terms: D000818:Animals; D003424:Crohn Disease / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*
chemical_list: D018076:DNA, Complementary; D012313:RNA; D014302:Trinitrobenzenesulfonic Acid