Mechanical compression affecting the thermal-induced conformational stability and denaturation temperature of human fibrinogen.
Thermal-induced conformational stability and changes in denaturation temperature of human fibrinogen (FBG) after different mechanical compressions were investigated by a simultaneous Fourier transform infrared microspectroscopy equipped with thermal analyzer (thermal FTIR microscopic system). The confocal Raman microspectroscopy was also applied to determine the thermal reversibility of solid FBG. FBG powder was pressed on one KBr pellet (1 KBr method) or sealed within two KBr pellets (2 KBr method) by different mechanical compressions. The result indicates that there was no marked difference in the thermal behavior for the solid FBG samples prepared by 1 KBr method in the heating process even under different mechanical compression pressures, in which the thermal-induced denaturation temperatures from native to denatured state were maintained constant at 66-67 degrees C. However, the denaturation temperature for the solid FBG samples prepared by 2 KBr method was shifted from 55 to 62 degrees C with the increase of mechanical compression pressure. A good linear correlation was also found between the denaturation temperature and mechanical compression pressure for FBG samples prepared by 2 KBr method. The solid FBG sample, whether prepared by 1 KBr or 2 KBr method, was also found to show the thermal-irreversible property.
Lin
Shan-Yang
SY
Biopharmaceutics Laboratory, Department of Medical Research and Education, Taipei Veterans General Hospital, Shih-Pai, Taipei, Taiwan, ROC. sylin@vghtpe.gov.tw
Hsieh
Tzu-Feng
TF
Wei
Yen-Shan
YS
Li
Mei-Jane
MJ
D001965:Bromides / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D005340:Fibrinogen / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D008968:Molecular Conformation; D017680:Potassium Compounds / Q000737:chemistry; D011208:Powders; D011312:Pressure; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D013696:Temperature; D013816:Thermodynamics
D001965:Bromides; D017680:Potassium Compounds; D011208:Powders; D005340:Fibrinogen; C039004:potassium bromide