Incorporation of ribonucleoside 5'-(alpha-P-borano)triphosphates into a 20-mer RNA by T7 RNA polymerase.
The enzymatic synthesis of short boranophosphate RNA was studied by comparing the yield and pattern of abortive products of in vitro transcription at steady-state conditions with that of normal RNA. Boranophosphate short RNA can be readily synthesized by T7 RNA polymerase.
Wan
Jing
J
Department of Chemistry, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina, USA.
Shaw
Barbara Ramsay
BR
D000255:Adenosine Triphosphate / Q000737:chemistry; D001880:Boranes / Q000138:chemical synthesis* / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry* / Q000494:pharmacology; D011487:Protein Conformation; D012313:RNA / Q000737:chemistry; D034741:RNA, Small Interfering / Q000737:chemistry; D012265:Ribonucleotides / Q000138:chemical synthesis*; D013997:Time Factors; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism
D001880:Boranes; D046911:Macromolecular Substances; D009841:Oligonucleotides; D010710:Phosphates; D034741:RNA, Small Interfering; D012265:Ribonucleotides; D014764:Viral Proteins; D012313:RNA; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases