title: Triple helical structure and stabilization of collagen-like molecules with 4(R)-hydroxyproline in the Xaa position.
abstract: In this study, we examine the relationships between the structure and stability of five related collagen-like molecules that have hydroxyproline residues occupying positions not observed in vertebrate collagen. Two of the molecules contain valine or threonine and form stable triple helices in water. Three of the molecules contain allo-threonine (an enantiomer of threonine), serine, or alanine, and are not stable. Using molecular dynamics simulation methods, we examine possible explanations for the stability difference, including considering the possibility that differences in solvent shielding of the essential interchain hydrogen bonds may result in differences in stability. By comparing the structures of threonine- and allo-threonine-containing molecules in six polar and nonpolar solvation conditions, we find that solvent shielding is not an adequate explanation for the stability difference. A closer examination of the peptides shows that the structures of the unstable molecules are looser, having weaker intermolecular hydrogen bonds. The weakened hydrogen bonds result from extended Yaa residue Psi-angles that prevent optimal geometry. The Phi-Psi-maps of the relevant residues suggest that each residue's most favorable Psi-angle determines the corresponding collagen-like molecule's stability. Additionally, we propose that these molecules illustrate a more general feature of triple-helical structures: interchain hydrogen bonds are always longer and weaker than ideal, so they are sensitive to relatively small changes in molecular structure. This sensitivity to small changes may explain why large stability differences often result from seemingly small changes in residue sequence.
authors.affiliation: Department of Genetics, School of Medicine, Stanford University, Stanford, California 94305, USA.
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Radmer
authors.forename: Teri E
authors.initials: TE
authors.lastname: Klein
mesh_terms: D003094:Collagen / Q000737:chemistry*; D003198:Computer Simulation; D006801:Humans; D006860:Hydrogen Bonding; D006909:Hydroxyproline / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D009154:Mutation; D011336:Probability; D011392:Proline / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013696:Temperature; D013912:Threonine / Q000737:chemistry; D014633:Valine / Q000737:chemistry
chemical_list: D013912:Threonine; D003094:Collagen; D011392:Proline; D014633:Valine; D006909:Hydroxyproline