Evaluation of Fusarium solani hyphae and conidia susceptibility to amphotericin B and itraconazole: study of a clinical case.
Fusarium species are hyaline moulds belonging to the hyalohyphomycosis group that are usually found in the soil and plants. This organism has emerged as a cause of disseminated invasive disease. The correlation between in vitro value and clinical efficacy is low and many patients remain unresponsive to treatment despite in vitro susceptibility. We determined growth control for Fusarium solani using the BioCell-Tracer system that measures the growth rate of a single fungal hypha, and the effect of different concentrations of amphotericin B and itraconazole. The MIC for these two drugs was also determined by a broth microdilution technique, using RPMI 1640. Different MICs for amphotericin B were obtained by the two different methods. This paper describes a case of infection due to Fusarium solani in an allogeneic bone marrow transplanted patient, the microbiological diagnostic, antifungal susceptibility tests for conidia and hypha and clinical correlation.
Teixeira
A B A
AB
Clinical Pathology Department, Medical Science Faculty, State University of Campinas, Cidade Universitaria Zeferino Vaz, CEP 13083-970 Campinas, São Paulo, Brazil.
Moretti
M L
ML
Trabasso
P
P
von Nowakonski
A
A
Aoki
F H
FH
Vigorito
A C
AC
Miyaji
M
M
Nishimura
K
K
Taguchi
H
H
Schreiber
A Z
AZ
D000328:Adult; D000666:Amphotericin B / Q000494:pharmacology* / Q000627:therapeutic use; D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D017809:Fatal Outcome; D005260:Female; D005670:Fusarium / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D016867:Immunocompromised Host; D009181:Mycoses / Q000188:drug therapy / Q000382:microbiology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology; D018805:Sepsis / Q000188:drug therapy / Q000382:microbiology*
D000935:Antifungal Agents; D004271:DNA, Fungal; D000666:Amphotericin B