[Analysis of secreted proteases of Trichophyton rubrum].
Secreted proteases are thought as potential virulent factors of Tricophyton rubrum. Based on cDNA libraries of 6 physiological phases of Tricophyton rubrum, 9683 unique ESTs were obtained by DNA sequencing. By bioinformatic analysis, 18 ESTs of putative secreted proteases were obtained from the established ESTs library, including 4 secreted peptidases, 1 secreted metalloprotease, 2 extracellular serine proteases, 1 secreted aspartic proteinase, 9 secreted subtilisin-like proteases and 1 vacuolar serine protease. These secreted proteases are related to nutrient uptake, lesion extension and host immune responses elicitation in the process of T. rubrum infection. These results provide a clue to further research on the infection and pathogencity of T. rubrum.
Leng
Wen-Chuan
WC
State Key Laboratory for Molecular Virology and Genetic Engineering, Chinese Center for Disease Control and Prevention, Beijing 100176, China. lengwenchuan@hotmail.com
Wang
Ling-Ling
LL
Wei
Can-Dong
CD
Yang
Jian
J
Jin
Qi
Q
D000595:Amino Acid Sequence; D020224:Expressed Sequence Tags; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D014249:Trichophyton / Q000201:enzymology* / Q000472:pathogenicity
D010447:Peptide Hydrolases