Differential protein expression profiles in anterior gills of Eriocheir sinensis during acclimation to cadmium.
Using a proteomic approach, we characterized different protein expression profiles in anterior gills of the Chinese mitten crab, Eriocheir sinensis, after cadmium (Cd) exposure. Two experimental conditions were tested: (i) an acute exposure (i.e. 500 microg Cd l(-1) for 3 days) for which physiological, biochemical and ultrastructural damage have been observed previously; (ii) a chronic exposure (i.e. 50 microg Cd l(-1) for 30 days) resulting in physiological acclimation, i.e. increased resistance to a subsequent acute exposure. Two-dimensional gel electrophoresis (2-DE) revealed six protein spots differentially expressed after acute, and 31 after chronic Cd exposure. From these spots, 15 protein species were identified using MS/MS micro-sequencing and MS BLAST database searches. Alpha tubulin, glutathione S-transferase and crustacean calcium-binding protein 23 were down-regulated after an acute exposure, whereas another glutathione S-transferase isoform was up-regulated. Furthermore, analyses revealed the over-expression of protein disulfide isomerase, thioredoxin peroxidase, glutathione S-transferase, a proteasome subunit and cathepsin D after chronic exposure. Under the same condition, ATP synthase beta, alpha tubulin, arginine kinase, glyceraldehyde-3-phosphate dehydrogenase and malate dehydrogenase were down-regulated. These results demonstrate that acute and chronic exposure to waterborne Cd induced different responses at the protein expression level. Protein identification supports the idea that Cd mainly exerts its toxicity through oxidative stress induction and sulfhydryl-group binding. As a result, analyses showed the up-regulation of several antioxidant enzymes and chaperonins during acclimation process. The gill proteolytic capacity seems also to be increased. On the other hand, the clearly decreased abundance of several enzymes involved in energy transfer suggests that chronic metal exposure induced an important metabolic reshuffling.
Silvestre
Frédéric
F
Unité de Recherche en Biologie des Organismes, Facultés Universitaires Notre-Dame de la Paix, rue de Bruxelles 61, B-5000 Namur, Belgium. frederic.silvestre@fundp.ac.be
Dierick
Jean-François
JF
Dumont
Valéry
V
Dieu
Marc
M
Raes
Martine
M
Devos
Pierre
P
D000064:Acclimatization / Q000502:physiology*; D000818:Animals; D003386:Brachyura / Q000187:drug effects* / Q000502:physiology*; D002104:Cadmium / Q000494:pharmacology*; D015536:Down-Regulation / Q000502:physiology; D004781:Environmental Exposure; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D005880:Gills / Q000187:drug effects / Q000502:physiology; D013058:Mass Spectrometry / Q000662:veterinary; D018384:Oxidative Stress / Q000502:physiology; D010101:Oxygen Consumption / Q000187:drug effects; D040901:Proteomics / Q000379:methods; D015854:Up-Regulation / Q000502:physiology; D014874:Water Pollutants, Chemical / Q000494:pharmacology
D014874:Water Pollutants, Chemical; D002104:Cadmium