Enhancement of the transmesothelial resistance of the parietal sheep peritoneum by epinephrine in vitro: ussing-type chamber experiments.
The peritoneal mesothelium constitutes an ion transport barrier that is taken advantage of in peritoneal dialysis. The aim of this study was to investigate the effects of epinephrine on the electrical transmesothelial resistance (R(TM)) of the isolated parietal sheep peritoneum by means of Ussing-type chamber experiments. Intact parietal (diaphragmatic) peritoneal samples were obtained from adult sheep immediately after sacrifice and transferred within 0.5 h to the laboratory in a cooled Krebs-Ringer bicarbonate solution (4 degrees C, pH 7.5), bubbled with 95% O2-5% CO2. A parietal peritoneal planar sheet was mounted in a Ussing-type chamber. Epinephrine (10(-7) M) was added to the apical and the basolateral side. The R(TM) was measured before and serially after the addition of epinephrine for 30 min. As active ion transport is temperature-dependent, all measurements were performed at 37 degrees C. The results were calculated as means with standard errors (x +/- SE) of six independent experiments. The control R(TM) was 20.05 +/- 0.61 ohm x cm2. The addition of epinephrine to the basolateral side within 1 min induced an increase of R(TM) to 21.8 +/- 0.45 ohm x cm2, which decreased thereafter progressively to reach control values again after 15 min. A similar effect of epinephrine on the apical side was apparent with a rapid rise of R(TM) to 22.5 +/- 0.66 ohm x cm2 and a subsequent decrease (P < 0.05). A clear association between the R(TM) and active ion transport was established from previous studies. The results of our study indicate a rapid action of epinephrine on the parietal peritoneum permeability.
Stefanidis
Ioannis
I
Division of Nephrology, University Hospital of Larissa, Larissa, Greece. stefanid@med.uth.gr
Zarogiannis
Sotirios
S
Hatzoglou
Chrissi
C
Liakopoulos
Vasilios
V
Kourti
Panagiota
P
Poultsidi
Antigoni
A
Mertens
Peter R
PR
Gourgoulianis
Konstantinos
K
Molyvdas
Paschalis-Adam
PA
D000818:Animals; D019149:Bioreactors; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004837:Epinephrine / Q000008:administration & dosage*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D010537:Peritoneum / Q000187:drug effects* / Q000502:physiology*; D010539:Permeability / Q000187:drug effects; D012756:Sheep
D004837:Epinephrine