Rat decidual cell cultures.
Pregnancy requires profound reorganization of the different tissues forming the uterus. Growth and differentiation of the uterine endometrial cells give rise to the decidual tissue, a transitory organ, which plays a key role in fetal survival. In this chapter, we describe a technique for the dispersion and the separation of the two different decidual cell subpopulations with high yield and viability. We also detail a cell culture method, which allows the maintenance of the function and life span of these highly purified decidual cells when cultured either separately or in a co-culture system.
Gu
Yan
Y
Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD, USA.
Gibori
Geula
G
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D003656:Decidua / Q000166:cytology*; D005260:Female; D011247:Pregnancy; D011555:Pseudopregnancy; D051381:Rats