title: Phase I clinical trial of a HER-2/neu peptide (E75) vaccine for the prevention of prostate-specific antigen recurrence in high-risk prostate cancer patients.
abstract: OBJECTIVE
  The E75 peptide is an immunogenic peptide from the HER-2/neu protein that is substantially expressed in prostate cancer. We are conducting a clinical trial of an E75/granulocyte macrophage colony-stimulating factor vaccine to prevent post-prostatectomy prostate-specific antigen (PSA) recurrences in high-risk prostate cancer (HRPC) patients.
  
  
  METHODS
  Prostate cancer patients at high risk for recurrence were prospectively evaluated and identified by the validated Center for Prostate Disease Research (CPDR)/CaPSURE high-risk equation. From these high-risk equation patients, 27 HER-2/neu-expressing prostate cancer patients were enrolled. HLA-A2+ patients (n = 17) were vaccinated, whereas HLA-A2- patients (n = 10) were followed as clinical controls. Local/systemic toxicities, immunologic responses, and time to recurrence were measured.
  
  
  RESULTS
  This vaccine is safe with only minor toxicities observed. Additionally, the vaccine is immunogenic with all patients showing both in vivo and in vitro phenotypic and functional immune responses, although variable. HLA-A2+ patients were found to have larger tumors, higher postoperative Gleason scores, and more high-risk CPDR scores than HLA-A2- patients. Despite these differences, disease-free survival was not different between the vaccinated HLA-A2+ patients and the HLA-A2- controls at a median follow up of 23 months. Three of the four vaccinated patients that recurred had rising PSAs at the initiation of the trial. Ex vivo phenotypic assays were predictive of recurrences and correlated in general with functional assays.
  
  
  CONCLUSIONS
  The E75 vaccine strategy is safe and effective in eliciting an immune response against the HER-2/neu protein in HRPC patients and may be useful as a preventive strategy against disease recurrence. Vaccination in response to a rising PSA may be too late.
authors.affiliation: Clinical Breast Care Project, Department of Surgery and Center for Prostate Disease Research, Department of Urology, Walter Reed Army Medical Center, Washington, District of Colombia, USA.
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Hueman
authors.forename: Zia A
authors.initials: ZA
authors.lastname: Dehqanzada
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Novak
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Gurney
authors.forename: Michael M
authors.initials: MM
authors.lastname: Woll
authors.forename: Gayle B
authors.initials: GB
authors.lastname: Ryan
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Storrer
authors.forename: Christine
authors.initials: C
authors.lastname: Fisher
authors.forename: David G
authors.initials: DG
authors.lastname: McLeod
authors.forename: Constantine G
authors.initials: CG
authors.lastname: Ioannides
authors.forename: Sathibalan
authors.initials: S
authors.lastname: Ponniah
authors.forename: George E
authors.initials: GE
authors.lastname: Peoples
mesh_terms: D000368:Aged; D019496:Cancer Vaccines / Q000276:immunology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D010446:Peptide Fragments / Q000276:immunology*; D011379:Prognosis; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry / Q000276:immunology*; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D019496:Cancer Vaccines; D015789:HLA-A2 Antigen; D007371:Interferon-gamma; D010446:Peptide Fragments; D017430:Prostate-Specific Antigen; D018719:Erb-b2 Receptor Tyrosine Kinases