title: Surface functionalization of degradable polymers by covalent grafting.
abstract: With a new non-destructive and solvent-free photografting technique, N-vinylpyrrolidone was covalently grafted onto the surfaces of degradable polymers; poly(l-lactide), poly(epsilon-caprolactone), poly(lactide-co-glycolide), and poly(trimethylene carbonate). The modified surfaces were characterized by XPS, ATR-FTIR, SEM, and cell growth tests. The wettability was markedly improved, as static contact angles changed from about 80 degrees for the pristine substrates to around 30 degrees after 30min of grafting. Well-defined surface topographies, such as micro-patterns, are preserved in the process since the graft layers are thin. The biological response, measured as cytotoxicity, showed that the modified films provide good substrates, comparable with optimized cell culture plastics, for the adhesion and proliferation of normal human keratinocytes and skin fibroblasts.
authors.affiliation: School of Chemical Science and Engineering, Royal Institute of Technology, Fibre and Polymer Technology, SE-100 44 Stockholm, Sweden.
authors.forename: Martina
authors.initials: M
authors.lastname: Källrot
authors.forename: Ulrica
authors.initials: U
authors.lastname: Edlund
authors.forename: A-C
authors.initials: AC
authors.lastname: Albertsson
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects; D001673:Biodegradation, Environmental; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D004146:Dioxanes / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D010777:Photochemistry; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D011098:Polyglactin 910 / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects; D011760:Pyrrolidinones / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects; D012867:Skin / Q000166:cytology; D013499:Surface Properties / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D001672:Biocompatible Materials; D004146:Dioxanes; D011091:Polyesters; D011108:Polymers; D011760:Pyrrolidinones; C016240:polycaprolactone; C059299:polytrimethylene carbonate; D011098:Polyglactin 910; C033616:poly(lactide); C042670:N-vinyl-2-pyrrolidinone