[Generation of chemical-inducible activation tagging T-DNA insertion lines of Arabidopsis thaliana].
Using an estrogen-inducible expression XVE (LexA-VP16-Estragon Receptor) system, we have generated approximately 40 000 independent T-DNA insertion lines of Arabidopsis thaliana. Segregation analyses of about 18000 lines indicated that 51.6% of them contain single T-DNA insertions and that the average insertion number is 1.38 copies per line. Mutants displaying a variety of morphological alterations were identified, including those that affect development of roots,hypocotyls, leaves, floral organs and seeds as well as the flowering time.
Zhang
Jian
J
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Xu
Jin-Xiang
JX
Kong
Ying-Zhen
YZ
Ji
Zhen-Dong
ZD
Wang
Xing-Chun
XC
An
Feng-Ying
FY
Li
Chao
C
Sun
Jia-Qiang
JQ
Zhang
Su-Zhi
SZ
Yang
Xiao-Hui
XH
Mu
Jin-Ye
JY
Liu
Xin-Fang
XF
Li
Jia-Yang
JY
Xue
Yong-Biao
YB
Zuo
Jian-Ru
JR
D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D004967:Estrogens / Q000494:pharmacology; D005822:Genetic Vectors / Q000235:genetics; D017405:Herpes Simplex Virus Protein Vmw65 / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000187:drug effects / Q000379:methods*; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics; D015533:Transcriptional Activation / Q000187:drug effects; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004967:Estrogens; D017405:Herpes Simplex Virus Protein Vmw65; D011960:Receptors, Estrogen; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein; C036483:T-DNA