Application of boron-entrapped stealth liposomes to inhibition of growth of tumour cells in the in vivo boron neutron-capture therapy model.
Tumour cell destruction in boron neutron-capture therapy (BNCT) is due to the nuclear reaction between (10)B and thermal neutrons. It is necessary for effective BNCT therapy to accumulate (10)B atoms in the tumour cells. The delivery system consisted of polyethylene-glycol (PEG) binding liposomes (DPPC/cholesterol/DSPC-PEG2000) with an entrapped (10)B-compound and we evaluated the cytotoxic effects of intravenously injected (10)B-PEG-liposomes on human pancreatic carcinoma xenografts in nude mice with thermal neutron irradiation. After thermal neutron irradiation of mice injected with (10)B-PEG-liposomes, growth of AsPC-1 tumours was suppressed relative to controls. Injection of (10)B-PEG-liposomes caused the greatest tumour suppression with thermal neutron irradiation in vivo. These results suggest that intravenous injection of (10)B-PEG-liposomes can increase the retention of (10)B atoms by tumour cells, causing suppression of tumour growth in vivo, after thermal neutron irradiation.
Yanagie
H
H
Department of Intellectual Property, Incubation Project: Inhibition of Cancer Metastasis, Research Centre for Advanced Science and Technology, The University of Tokyo, Japan. yanagie@ip.rcast.u.tokyo.ac.jp
Maruyama
K
K
Takizawa
T
T
Ishida
O
O
Ogura
K
K
Matsumoto
T
T
Sakurai
Y
Y
Kobayashi
T
T
Shinohara
A
A
Rant
J
J
Skvarc
J
J
Ilic
R
R
Kuhne
G
G
Chiba
M
M
Furuya
Y
Y
Sugiyama
H
H
Hisa
T
T
Ono
K
K
Kobayashi
H
H
Eriguchi
M
M
D000818:Animals; D001894:Borohydrides / Q000008:administration & dosage*; D001895:Boron / Q000008:administration & dosage*; D016754:Boron Neutron Capture Therapy; D045744:Cell Line, Tumor; D006801:Humans; D007554:Isotopes; D008081:Liposomes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D023421:Models, Animal; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D011092:Polyethylene Glycols / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000008:administration & dosage*
D001894:Borohydrides; D007554:Isotopes; D008081:Liposomes; D013438:Sulfhydryl Compounds; C014651:mercaptoundecahydrododecaborate; D011092:Polyethylene Glycols; D001895:Boron