Comparison of different transvaginal ovum pick-up protocols to optimise oocyte retrieval and embryo production over a 10-week period in cows.
The objective was to develop a simple and effective ovum pick-up (OPU) protocol for cows, optimised for oocyte harvest and subsequent in vitro embryo production (IVP). Five protocols differing in collection frequency, dominant follicle removal (DFR) and FSH stimulation were tested on groups of three cows each, over an interval of 10 consecutive weeks. Performance was evaluated on per OPU session, per week and pooled (3 cowsx10weeks) basis. Among the non-stimulated groups, on a per cow per session basis, once- or twice-weekly OPU had no effect on the mean (+/- S.E.M.) number of follicles aspirated, oocytes retrieved and blastocysts produced (0.6+/-0.8 and 0.7 +/- 0.7, respectively). However, DFR 72 h prior to OPU almost doubled blastocyst production (1.2 +/- 1.3). In stimulated groups, FSH treatment (80 mg IM and 120 mg SC) was given once weekly prior to OPU. Treatment with FSH, followed by twice-weekly OPU, failed to show any synergistic effect of FSH and increased aspiration frequency. When FSH was given 36 h after DFR, followed by OPU 48 h later, more (P < 0.05) follicles (16.0 +/- 5.0), oocytes (10.6 +/- 4.5) and embryos (2.1 +/- 1.2) were obtained during each session, but not on a weekly basis. Pooled results over 10 weeks showed an overall improved performance for the treatment groups with twice-weekly OPU sessions, due to double the number of OPU sessions performed. However, the protocol that consisted of DFR, FSH treatment and a subsequent single OPU per week, was the most productive and cost-effective, with potential commercial appeal.
Chaubal
S A
SA
Center for Regenerative Biology/Department of Animal Science, University of Connecticut, 1392 Storrs Rd., Storrs, CT 06269-4243, USA.
Molina
J A
JA
Ohlrichs
C L
CL
Ferre
L B
LB
Faber
D C
DC
Bols
P E J
PE
Riesen
J W
JW
Tian
X
X
Yang
X
X
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology*; D001947:Breeding / Q000379:methods; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology; D010063:Ovum / Q000166:cytology*; D011247:Pregnancy; D011897:Random Allocation; D027724:Reproductive Techniques, Assisted / Q000662:veterinary*; D013997:Time Factors
D005640:Follicle Stimulating Hormone