Effects of acrolein on the quadruplex forming d(TTAGGG)4 telomeric repeat sequence.
HPLC and ESI-MS analysis have been used to investigate the effect of acrolein exposure on d(TITAGGG)4 human telomeric repeat. Preliminary results disclosed a novel relationship between the structure assumed by oligodeoxynucleotides (ODNs) and the capability of their nucleobase residues to react with acrolein.
Borbone
Nicola
N
Dipartimento di Chimica delle Sostanze Naturali, University of Naples, Faculty of Pharmacy, via Domenico Montesano 49, Napoli 80131, Italy. borbone@unina.it
D'Isa
Giuliana
G
Oliviero
Giorgia
G
Piccialli
Gennaro
G
Varra
Michela
M
Mayol
Luciano
L
D000171:Acrolein / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides / Q000737:chemistry*; D012091:Repetitive Sequences, Nucleic Acid; D021241:Spectrometry, Mass, Electrospray Ionization; D016615:Telomere / Q000648:ultrastructure*
D009692:Nucleic Acid Heteroduplexes; D009841:Oligonucleotides; D000171:Acrolein; D004247:DNA