Carbohydrate array analysis of anti-Tn antibodies and lectins reveals unexpected specificities: implications for diagnostic and vaccine development.
The Tn antigen is a carbohydrate antigen expressed in most carcinomas, during embryogenesis, on pathogenic parasites, and on HIV. It has been evaluated extensively as a potential diagnostic marker and several Tn-based vaccines are in clinical trials. Based on discrepancies in the literature regarding Tn expression, we began to question whether antibodies and lectins used routinely to detect the Tn antigen were providing accurate information. To investigate this possibility, a carbohydrate microarray and a highly sensitive assay were developed and three frequently used Tn receptors (HBTn1, Bric111, and VVL-B4) were evaluated. Carbohydrate-array analysis revealed unexpected cross-reactivity with other human carbohydrate epitopes. VVL-B4 bound the Tn antigen, GalNAcalpha1-6Gal, and GalNAcalpha1-3Gal. Bric111 bound the Tn antigen, blood group A, GalNAcalpha1-6Gal, and GalNAcalpha1-3Gal. HBTn1 showed the best selectivity, but still displayed moderate binding to blood group A. Implications for the development of Tn-based diagnostics and vaccines are discussed.
Manimala
Joseph C
JC
Laboratory of Medicinal Chemistry, Center for Cancer Research, NCI-Frederick, 376 Boyles Street, Building 376, Room109, Frederick, MD 21702, USA.
Li
Zhitao
Z
Jain
Amit
A
VedBrat
Sharanjeet
S
Gildersleeve
Jeffrey C
JC
D000906:Antibodies / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D002241:Carbohydrates; D019937:Diagnostic Techniques and Procedures; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D006801:Humans; D037102:Lectins / Q000032:analysis*; D046228:Microarray Analysis; D012680:Sensitivity and Specificity; D014612:Vaccines
D000906:Antibodies; D015295:Antigens, Tumor-Associated, Carbohydrate; D002241:Carbohydrates; D037102:Lectins; C040286:Tn antigen; D014612:Vaccines