Hypoxia level and matrix metalloproteinases-2 and -9 activity in Lewis lung carcinoma: correlation with metastasis.
OBJECTIVE
To investigate the relationship between tumor hypoxia in vivo, activity of matrix metalloproteinases (MMPs), and metastatic potential of tumor.


METHODS
Lewis lung carcinoma (3LL) was used in this study. Total activity of MMP-2 and -9 in tumor was measured biochemically, tumor hypoxia level was assessed by (31)P NMR spectroscopy in tissue perchloric extracts.


RESULTS
It was determined that hypoxia level in primary tumor has been concomitantly increasing along with tumor growth and correlated with metastasis level in lung. The positive correlation between hypoxia level and activities of MMP-2 and MMP-9 in primary tumor was registered. Moreover, the activity of MMP-2 and -9 in 3LL (primary tumor) directly correlates with metastasis level in lung.


CONCLUSIONS
This study demonstrated that the growth of primary tumor is distinctly accompanied by an increase of tumor hypoxia level which positively correlates both with the activity of MMP-2 and -9 in primary tumor and metastatic efficiency.
Osinsky
S P
SP
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences, Kiev 03022, Ukraine. osion@onconet.kiev.ua
Ganusevich
I I
II
Bubnovskaya
L N
LN
Valkovskaya
N V
NV
Kovelskaya
A V
AV
Sergienko
T K
TK
Zimina
S V
SV
D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000503:physiopathology*; D015687:Cell Hypoxia; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000503:physiopathology*
D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9