title: Babesia canis vogeli: a novel PCR for its detection in dogs in Australia.
abstract: Babesia canis vogeli is known to cause disease in dogs in Australia, and the rapid detection of various subspecies would enable effective treatment and management. A 21 bp oligonucleotide, "Bab-f" was proposed for the production of larger PCR products with high species specificity that would enable effective sequence analyses to yield subspecies identification. The new forward primer when paired with a previously reported "Babesia common" reverse primer generated a 394 bp product which was successfully amplified and provided subspecies differentiation by sequence analyses. Specificity and sensitivity were reported at 100% on a cohort of 55 dogs.
authors.affiliation: Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia.
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Martin
authors.forename: Richard H
authors.initials: RH
authors.lastname: Dunstan
authors.forename: Timothy K
authors.initials: TK
authors.lastname: Roberts
authors.forename: Graeme K
authors.initials: GK
authors.lastname: Brown
mesh_terms: D000818:Animals; D001315:Australia; D001403:Babesia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001404:Babesiosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D015331:Cohort Studies; D017931:DNA Primers; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification*; D004283:Dog Diseases / Q000175:diagnosis / Q000469:parasitology*; D004285:Dogs; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards / Q000662:veterinary*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012680:Sensitivity and Specificity; D016415:Sequence Alignment / Q000662:veterinary; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S