[Determination of two main components in bark of Paeonia suffruticosa by HPLC].
OBJECTIVE
To establish a HPLC method for determination two constituents in bark of Paeonia Suffuticosa.


METHODS
The reversed phase HPLC system consisting of an Alltima ODS column (4.6 mm x 150 mm, 5 microm) and a mixture of water-THF-methanol-HAc (60:20:20:0.05) as the mobile phase was used. The flow rate was 0.8 mL x min(-1) and UV detection was set at 274 nm.


RESULTS
The assay displayed good linearity over the concentration ranges of 0.06-1.0 microg (r = 0.999 9, gallic acid) and 0.16-2.58 microg (r = 0.999 9, paeonol) respectively. The average recoveries (n = 9) of gallic acid and paeonol were 98.6% (RSD = 3.0%), 98.2% (RSD = 2.5%), respectively. The samples were extracted with methanol for 24 h bu maceration.


CONCLUSIONS
The method is simple, accurate and can be used for the quality study of bark of P. suffruticosa.
Tian
Yuan-zi
YZ
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Befiing 100050, China.
Wang
Yan
Y
Liu
Jian-zhen
JZ
Yang
Bei-bei
BB
Shi
Jian-gong
JG
Wang
Mu-zou
MZ
D000098:Acetophenones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005707:Gallic Acid / Q000032:analysis*; D029596:Paeonia / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control
D000098:Acetophenones; C013638:paeonol; D005707:Gallic Acid