Ultra-rapid separation of an angiotensin mixture in nanochannels using shear-driven chromatography.
The present paper reports on the separation of a mixture of fluorescein isothiocyanate-labeled angiotensin I and II peptides in a shear-driven nanochannel with a C18-coating and using an eluent consisting of 5% acetonitrile in 0.02 M aqueous phosphate buffer at pH 6.5. The flat-rectangular nanochannel in fused silica consisted of an etched structure in combination with a flat moving wall. The very fast separation kinetics that can be achieved in a nanochannel allowed to separate the angiotensin peptides in less then 0.2 s in a distance of only 1.8 mm. Plate heights as small as 0.4 microm were calculated after substraction of the injection effect.
Vankrunkelsven
Sarah
S
Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium. svkrunke@vub.ac.be
Clicq
David
D
Cabooter
Deirdre
D
De Malsche
Wim
W
Gardeniers
J G E
JG
Desmet
Gert
G
D000803:Angiotensin I / Q000302:isolation & purification*; D000804:Angiotensin II / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D036103:Nanotechnology
D000804:Angiotensin II; D000803:Angiotensin I