Increased interleukin-18 content and angiogenic activity of sera from diabetic (Type 2) patients with background retinopathy.
Hyperglycaemia increases inflammatory cytokine concentration in the blood. Elevated levels of interleukin-18 (IL-18), a cytokine belonging to the interleukin-1 (IL-1) family, were recently reported in patients with Type 2 diabetes mellitus (DM2) and nephropathy. The aim of the present work was an examination of IL-18 concentration in the sera of elderly DM2 patients with nonproliferative retinopathy and age-matched control people and an estimation whether this cytokine plays pro- or anti-angiogenic role in in vivo angiogenic activity of their sera in mice cutaneous angiogenesis test. Recombinant human IL-18 injected intradermally to murine skin induced significant neovascular reaction. DM2 patients sera contained higher concentration of IL-18 and induced stronger neovascular reaction in mice skin than did the sera of corresponding control people. Sera from both groups of people after neutralization with antihuman IL-18 antibodies lost substantial part of their angiogenic activity.
Skopiński
Piotr
P
Department of Ophthalmology, 2nd Faculty of Medicine, Warsaw Medical University, Poland. pskopin@ib.amwaw.edu.pl
Rogala
Ewa
E
Duda-Król
Barbara
B
Lipińska
Anna
A
Sommer
Ewa
E
Chorostowska-Wynimko
Joanna
J
Szaflik
Jerzy
J
Partyka
Iwona
I
Skopińska-Rózewska
Ewa
E
D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D003930:Diabetic Retinopathy / Q000097:blood*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D020382:Interleukin-18 / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D015861:Retinal Neovascularization / Q000097:blood*; D012867:Skin / Q000098:blood supply
D006442:Glycated Hemoglobin; D020382:Interleukin-18