A hind limb tourniquet induces interleukin-6 expression in a rat dorsal root ganglion.
We investigated the mRNA levels of interleukin-6-related genes in a rat dorsal root ganglion after application of a tourniquet to a hind limb in order to identify the molecules that are induced immediately after peripheral nerve injury at the early stage. Induction of interleukin-6 and upregulation of glycoprotein 130 mRNA expressions were observed in the ipsilateral dorsal root ganglion at 4 h after tourniquet application. Interleukin-6 protein was detected in small-sized and medium-sized dorsal root ganglion cells by immunohistochemical analysis. The induction of interleukin-6 expression is likely to play a role in the protection of injured neurons perhaps related to growth of their axons. Glycoprotein 130 might also account for the inhibitory effects following nerve injury.
Abe
Sumiko
S
Department of Legal Medicine, Fukushima Medical University School of Medicine, Hikarigaoka, Fukushima, Japan. sumiko@fmu.ac.jp
Mizusawa
Ikubumi
I
Suto
Miwako
M
Kato
Naho
N
Yabashi
Atsuko
A
Honda
Takashi
T
Hiraiwa
Kouichi
K
D000818:Animals; D050822:Cytokine Receptor gp130 / Q000235:genetics* / Q000378:metabolism; D005727:Ganglia, Spinal / Q000502:physiology*; D015870:Gene Expression; D006614:Hindlimb; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D018482:Muscle, Skeletal / Q000294:innervation; D009416:Nerve Regeneration; D009433:Neural Inhibition; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012584:Sciatic Nerve / Q000293:injuries*; D014111:Tourniquets
D015850:Interleukin-6; D012333:RNA, Messenger; D050822:Cytokine Receptor gp130