The ultrastructure of hybrid acute leukemia: a study of 15 cases.
The objective of this study was to investigate the ultrastructural characteristics of hybrid acute leukemia (HAL). Fifteen cases of HAL were studied by transmission electron microscopy (TEM), focusing on organelles and myeloperoxidase (MPO) reaction of leukemic cells. By TEM, 5 out 15 cases of HAL were consistent with immunophenotyping (3 cases of biphenotypic type, and 2 cases of biclonal type with granulocytes and lymphocytes); 2 cases were suspected as HAL. On other hand, 5 cases of HAL were assigned to ALL, and 2 cases were misinterpreted as M5a and 1 as M4b. Most of the blast cells of biphenotypic HAL showed lymphoid features, except some cases containing MPO positive granules in blasts, while a few cases exhibited monocytic or nonspecific features. TEM offers advantages in the diagnosis of biclonal type HAL and biphenotypic HAL positive for MPO. However, it is difficult to differentiate MPO-negative cases of biphenotypic HAL from ALL and a few cases may be misinterpreted as M5 by TEM.
Ru
Yong-Xin
YX
Institute of Hematology & Blood Diseases Hospital, Peking Union College, Tianjin, China.
Wang
Hui-Jun
HJ
Yang
Bing-Xin
BX
Liu
Jin-Hua
JH
Li
Zhan-Qi
ZQ
Li
Cheng-Wen
CW
Wang
Jian-Xiang
JX
Mi
Ying-Chang
YC
D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D042381:Granulocyte Precursor Cells / Q000201:enzymology / Q000648:ultrastructure*; D006801:Humans; D016130:Immunophenotyping; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D008214:Lymphocytes / Q000201:enzymology / Q000648:ultrastructure*; D008297:Male; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D009195:Peroxidase / Q000378:metabolism; D010641:Phenotype; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology
D009195:Peroxidase