Intracellular interference of infectious bursal disease virus.
A search for dominant-negative mutant polypeptides hampering infectious bursal disease virus (IBDV) replication has been undertaken. We have found that expression of a mutant version of the VP3 structural polypeptide known as VP3/M3, partially lacking the domain responsible for the interaction with the virus-encoded RNA polymerase, efficiently interferes with the IBDV replication cycle. Transformed cells stably expressing VP3/M3 show a significant reduction (up to 96%) in their ability to support IBDV growth. Our findings provide a new tool for the characterization of the IBDV replication cycle and might facilitate the generation of genetically modified chicken lines with a reduced susceptibility to IBDV infection.
González
Dolores
D
Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología, Madrid, Spain. jfrodrig@cnb.uam.es.
Rodríguez
Jose Francisco
JF
Abaitua
Fernando
F
D018175:Birnaviridae Infections / Q000503:physiopathology; D002460:Cell Line; D017353:Gene Deletion; D006801:Humans; D007243:Infectious bursal disease virus / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D015678:Viral Structural Proteins / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
C478244:VP3 protein, infectious bursal disease virus; D015678:Viral Structural Proteins