Characterization of ORF2 and its encoded protein of the Helicoverpa armigera nucleopolyhedrovirus.
The open reading frame 2 (ha2) of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV), a conserved gene in most baculoviruses from lepidopteran insects such as p78/83 of the Autographa californica MNPV, was characterized. It is 1,242 bp long and potentially encodes a 45.9 kDa. Ha2 is conserved among baculoviruses from lepidopteran insects. Ha2 transcripts were detected from 16 to 96 h post infection (hpi) of HzAM1 cells. Rabbit polyclonal antiserum against a GST-HA2 fusion protein reacted with three protein of 50, 46 and 35 kDa at 24-72 hpi of HzAM1 cells. Anti OpMNPV ORF2 (homologue of HA2) antibody reacted only with the 46 and 35 kDa proteins in HaSNPV-infected cells. These results demonstrate that Ha2 is modified at the mRNA or protein levels. Western blot analysis showed that only the 50 kDa product of HA2 is a structural component of proteins of both the budded virus (BV) and occlusion-derived virus (ODV) phenotypes. HA2-EGFP fusion protein showed that HA2 is localized primarily in the nucleus of HzAM1 infected cells. The HA2 was found to co-localize with actin by labelling of actin with Rhordamine-Phalloidin. In summary, the data indicated that HA2 is a structural protein and interacts with host cell actin.
Nie
Yingchao
Y
State Key Lab of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China.
Wang
Qian
Q
Liang
Changyong
C
Fang
Minggang
M
Yu
Zehua
Z
Chen
Xinwen
X
D000199:Actins / Q000032:analysis; D000818:Animals; D019677:Artificial Gene Fusion; D015153:Blotting, Western; D002460:Cell Line; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics; D018613:Microscopy, Confocal; D008970:Molecular Weight; D009036:Moths / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016366:Open Reading Frames; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D018411:Spodoptera; D014764:Viral Proteins / Q000235:genetics* / Q000502:physiology; D015678:Viral Structural Proteins / Q000235:genetics*
D000199:Actins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins; D049452:Green Fluorescent Proteins