Isolation and characterization of anthocyanin 5-O-glucosyltransferase in Perilla frutescens var. crispa by differential display.
The glucosylation of 5-position of anthocyanin molecules is one of most important modification step, which is responsible for the stability and color hue of anthocyanin molecules. However, anthocyanin 5-O-glucosyltransferase (5-GT) is unstable and it had been difficult to purify this enzyme by biochemical methods. The isolation of cDNA encoding 5-GT was succeeded first by mRNA differential display (DD) of red and green chemotypes of Perilla frutescens var. crispa. The full-length cDNA was isolated by the screening of perilla cDNA library with cDNA fragments, which was displayed specifically in red perilla. The biological characters of this enzyme were investigated by using the recombinant protein expressed in yeast. We have shown an example of the application of DD technique to the molecular biology in plant secondary metabolisms.
Yamazaki
Mami
M
Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University, Japan.
Saito
Kazuki
K
D000872:Anthocyanins / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D005964:Glucosyltransferases / Q000737:chemistry* / Q000235:genetics*; D008956:Models, Chemical; D009693:Nucleic Acid Hybridization; D031344:Perilla frutescens / Q000201:enzymology*; D011994:Recombinant Proteins / Q000737:chemistry; D013997:Time Factors
D000872:Anthocyanins; D018076:DNA, Complementary; D011994:Recombinant Proteins; D005964:Glucosyltransferases; C118149:UDP-glucose anthocyanin 5-O-glucosyltransferase