title: Electrochemical behavior and detection of plant hormone 6-benzyl adenine in acetate buffer at mercury electrode.
abstract: The electrochemical behavior of 6-benzyl adenine (6-BA) has been studied in 0.1 mol L(-1) HAc-NaAc solution (pH 3.8). Cyclic voltammetry, single-sweep polarography and direct current polarography were employed to clarify the mechanism of the electrode process; the kinetic parameters of the rate-determining step were determined. Reduction of 6-BA involves two pH-dependent processes, corresponding to the overall irreversible reduction of the 1,6 and 3,2 N=C bonds. Each reduction stage consists a preprotonation of the nitrogen atom at the electroactive site and a rapid two-electron transfer. In the presence of 6-BA, the reduction potentials of some ions were shifted. Under the given conditions, 6-BA displays one irreversible reduction peak controlled by adsorption. Two linear response were observed in the range 2.0 x 10(-8) - 8.0 x 10(-7) mol L(-1) and the range 1.0 x 10(-6) - 8.0 x 10(-6) mol L(-1), with correlation coefficients of 0.9995 and 0.9998, respectively. The detection limit is 7.1 x 10(-9) mol L(-1). The method had been applied to the determination of 6-BA in bean sprout samples with satisfactory results.
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, P R. China. xwp@sdau.edu.cn
authors.forename: Xiang-Jin
authors.initials: XJ
authors.lastname: Qu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Ji-Gang
authors.initials: JG
authors.lastname: Gao
mesh_terms: D019342:Acetic Acid / Q000737:chemistry* / Q000494:pharmacology; D001593:Benzyl Compounds; D002021:Buffers; D004563:Electrochemistry; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D007701:Kinetin / Q000032:analysis*; D008628:Mercury / Q000737:chemistry*; D010084:Oxidation-Reduction; D010937:Plant Growth Regulators / Q000032:analysis*; D011687:Purines; D019346:Sodium Acetate / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001593:Benzyl Compounds; D002021:Buffers; D010937:Plant Growth Regulators; D011687:Purines; D019346:Sodium Acetate; D008628:Mercury; C480551:benzylaminopurine; D007701:Kinetin; D019342:Acetic Acid