title: The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K.
abstract: Lactobacillus sakei is a psychotrophic lactic acid bacterium found naturally on fresh meat and fish. This microorganism is widely used in the manufacture of fermented meats and has biotechnological potential in biopreservation and food safety. We have explored the 1,884,661-base-pair (bp) circular chromosome of strain 23K encoding 1,883 predicted genes. Genome sequencing revealed a specialized metabolic repertoire, including purine nucleoside scavenging that may contribute to an ability to successfully compete on raw meat products. Many genes appear responsible for robustness during the rigors of food processing--particularly resilience against changing redox and oxygen levels. Genes potentially responsible for biofilm formation and cellular aggregation that may assist the organism to colonize meat surfaces were also identified. This genome project is an initial step for investigating new biotechnological approaches to meat and fish processing and for exploring fundamental aspects of bacterial adaptation to these specific environments.
authors.affiliation: Unité Flore Lactique et Environnement Carné, INRA Domaine de Vilvert, 78350 Jouy-en-Josas, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Chaillou
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Champomier-Vergès
authors.forename: Monique
authors.initials: M
authors.lastname: Cornet
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Crutz-Le Coq
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Dudez
authors.forename: Véronique
authors.initials: V
authors.lastname: Martin
authors.forename: Sophie
authors.initials: S
authors.lastname: Beaufils
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Darbon-Rongère
authors.forename: Robert
authors.initials: R
authors.lastname: Bossy
authors.forename: Valentin
authors.initials: V
authors.lastname: Loux
authors.forename: Monique
authors.initials: M
authors.lastname: Zagorec
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002874:Chromosome Mapping; D005516:Food Microbiology; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology; D016680:Genome, Bacterial / Q000235:genetics*; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000235:genetics* / Q000378:metabolism*; D008460:Meat / Q000382:microbiology*; D008969:Molecular Sequence Data; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D019344:Lactic Acid