Relationship between cytokine gene polymorphisms and graft-versus-host disease after allogeneic stem cell transplantation in a Brazilian population.
Graft-versus-host disease (GVHD) is the major complication of allogeneic hematopoietic stem cell transplantation (HSCT), and cytokines are recognized as important mediators of GVHD. Polymorphisms in the regulatory regions of several cytokine genes have been associated with a number of immune diseases as well as organ transplant complications. In this study we have investigated the role of tumor necrosis factor-alpha(-308), interleukin (IL)-6(-174), IL-10(-1082, -819, -592), Interferon-gamma(-874), and transforming growth factor-beta1(+869, +915) polymorphisms on HSCT outcome. Donor/recipient genotypes were analyzed by polymerase chain reaction with sequence specific primers (PCR-SSP). Although we have found a small number of low IL-6, a polymorphism at position -174 of the recipient and donor IL-6 gene was associated with the increased incidence of chronic GVHD. Therefore, this study emphasizes the probable potential role of genetic variability of donor and recipient in determining outcome after transplantation.
Laguila Visentainer
Jeane Eliete
JE
Department of Clinical Analyses, Maringá State University, Av. Colombo, 5790, Maringá, CEP 87020-900, PR, Brazil. jelvisentainer@uem.br
Lieber
Sofia Rocha
SR
Lopes Persoli
Lígia Beatriz
LB
Dutra Marques
Sílvia Barbosa
SB
Vigorito
Afonso Celso
AC
Penteado Aranha
Francisco José
FJ
de Brito Eid
Kátia Aparecida
KA
Oliveira
Gislaine Borba
GB
Martins Miranda
Eliana Cristina
EC
Bragotto
Luciana
L
de Souza
Cármino Antonio
CA
D000293:Adolescent; D000328:Adult; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000235:genetics*; D014644:Genetic Variation; D006086:Graft vs Host Disease / Q000235:genetics* / Q000276:immunology / Q000401:mortality; D018380:Hematopoietic Stem Cell Transplantation / Q000401:mortality; D006412:Hematopoietic Stem Cells; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D014184:Transplantation, Homologous
D016207:Cytokines