title: Role of the precorrin 6-X reductase gene in cobamide biosynthesis in Methanococcus maripaludis.
abstract: In Methanococcus maripaludis strain JJ, deletion of the homolog to cbiJ, which encodes the corrin biosynthetic enzyme precorrin 6-X reductase, yielded an auxotroph that required either cobamide or acetate for good growth. This phenotype closely resembled that of JJ117, a mutant in which tandem repeats were introduced into the region immediately downstream of the homolog of cbiJ. Mutant JJ117 also produced low quantities of cobamides, about 15 nmol g(-1) protein or 1-2% of the amount found in wild-type cells. These results confirm the role of the cbiJ homolog in cobamide biosynthesis in the Archaea and suggest the presence of low amounts of a bypass activity in these organisms.
authors.affiliation: Department of Microbiology, The Ohio State University, Columbus, OH, USA.
authors.forename: Wonduck
authors.initials: W
authors.lastname: Kim
authors.forename: Tiffany A
authors.initials: TA
authors.lastname: Major
authors.forename: William B
authors.initials: WB
authors.lastname: Whitman
mesh_terms: D001483:Base Sequence; D003038:Cobamides / Q000096:biosynthesis*; D019766:Genes, Archaeal; D017017:Methanococcus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism
chemical_list: D003038:Cobamides; D010088:Oxidoreductases; C072472:precorrin 6x oxidoreductase