[Rhinovirus and acute bronchiolitis in young infants].
OBJECTIVE
To determine the prevalence of rhinovirus infection in hospitalized young infants with acute bronchiolitis.


METHODS
Hospitalized children with acute bronchiolitis admitted to the Hospital São Lucas/PUCRS between May and September 2002 were selected prospectively. Nasopharyngeal samples were assayed for respiratory syncytial virus, parainfluenza, influenza and adenovirus by immunofluorescence. For rhinovirus test a reverse transcription-polymerase chain reaction for picornavirus was used, followed by hybridization with rhinovirus specific probes.


RESULTS
Forty-five patients were selected for the study. The median age of the subjects studied was 2 months. Positive samples for respiratory viruses were found in 35/45 (77.8%) subjects and 7/35 (20%) patients had dual infection. Respiratory syncytial virus was detected in 33/35 (94%) cases. Rhinovirus was detected in 6/35 patients (17%).


CONCLUSIONS
Rhinovirus was the second most common agent detected in nasal secretions from young infants hospitalized with acute bronchiolitis.
Pitrez
Paulo M C
PM
Departamento de Pediatria, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil. pmpitrez@pucrs.br
Stein
Renato T
RT
Stuermer
Larissa
L
Macedo
Izolete S
IS
Schmitt
Virgínia M
VM
Jones
Marcus H
MH
Arruda
Eurico
E
D000208:Acute Disease; D001938:Brazil / Q000453:epidemiology; D001988:Bronchiolitis / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010361:Patients; D010850:Picornaviridae Infections / Q000453:epidemiology*; D015995:Prevalence; D011446:Prospective Studies; D012135:Respiratory Sounds; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology*; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012229:Rhinovirus / Q000302:isolation & purification; D018709:Statistics, Nonparametric