title: Correlation of HSP70 expression and cell viability following thermal stimulation of bovine aortic endothelial cells.
abstract: Thermal preconditioning protocols for cardiac cells were identified which produce elevated HSP70 levels while maintaining high cell viability. Bovine aortic endothelial cells were heated with a water bath at temperatures ranging from 44 to 50 degrees C for periods of 1-30 min. Thermal stimulation protocols were determined which induce HSP70 expression levels ranging from 2.3 to 3.6 times the control while maintaining cell viabilities greater than 90%. An Arrhenius injury model fit to the cell damage data yielded values of A = 1.4 X 10(66) s(-1) and Ea = 4.1 X 10(5) J/mol. Knowledge of the injury parameters and HSP70 kinetics will enhance dosimetry guideline development for thermal stimulation of heat shock proteins expression in cardiac tissue.
authors.affiliation: The University of Texas at Austin, Biomedical Engineering, 1 University Station C0800, Austin, TX 78712-0238, USA. n.forney@mail.utexas.edu
authors.forename: Marissa Nichole
authors.initials: MN
authors.lastname: Rylander
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Diller
authors.forename: Sihong
authors.initials: S
authors.lastname: Wang
authors.forename: Shanti J
authors.initials: SJ
authors.lastname: Aggarwal
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000502:physiology; D017209:Apoptosis / Q000502:physiology*; D002417:Cattle; D002470:Cell Survival / Q000502:physiology*; D002478:Cells, Cultured; D003198:Computer Simulation; D042783:Endothelial Cells / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response / Q000502:physiology*; D006358:Hot Temperature; D008954:Models, Biological; D013223:Statistics as Topic
chemical_list: D018840:HSP70 Heat-Shock Proteins