Focal adhesion molecules as potential target of lead toxicity in NRK-52E cell line.
In this study, we investigated the influence of inorganic lead (Pb(II)), an environmental pollutant having nephrotoxic action, on the focal adhesion (FA) organization of a rat kidney epithelial cell line (NRK-52E). In particular, we evaluated the effects of the metal on the recruitment of paxillin, focal adhesion kinase, vinculin and cytoskeleton proteins at the FAs complexes. We provided evidences that, in proliferating NRK-52E cell cultures, low concentrations of Pb(II) affect the cell adhesive ability and stimulate the disassembly of FAs, thus inhibiting the integrin-activated signalling. These effects appeared to be strictly associated to the Pb-induced arrest of cell cycle at G0/G1 phase also proved in this cell line.
Giuliani
Roberta
R
Unit of Biochemistry, School of Medicine, University of Brescia, Italy.
Bettoni
Francesca
F
Leali
Daria
D
Morandini
Fausta
F
Apostoli
Pietro
P
Grigolato
Piergiovanni
P
Cesana
Bruno Mario
BM
Aleo
Maria Francesca
MF
D000199:Actins / Q000187:drug effects; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000378:metabolism*; D004785:Environmental Pollutants / Q000633:toxicity*; D004847:Epithelial Cells / Q000737:chemistry / Q000187:drug effects; D022001:Focal Adhesions / Q000187:drug effects*; D007668:Kidney / Q000166:cytology / Q000187:drug effects*; D007854:Lead / Q000633:toxicity*; D008870:Microtubules / Q000187:drug effects; D051381:Rats
D000199:Actins; D003598:Cytoskeletal Proteins; D004785:Environmental Pollutants; D007854:Lead; C029891:lead chloride