Surfactin C inhibits the lipopolysaccharide-induced transcription of interleukin-1beta and inducible nitric oxide synthase and nitric oxide production in murine RAW 264.7 cells.
The anti-inflammatory activity of the surfactin C derived from Bacillus subtilis isolate was investigated in lipopolysaccharide (LPS, 1 microg ml(-1))-treated mouse RAW 264.7 cells. LPS increased mRNA transcription of cyclooxygenase (COX)-2, interleukin (IL)-1beta and inducible nitric oxide synthase (iNOS). However, surfactin C at 50 microg ml(-1 )inhibited the LPS-induced increase in the transcription of IL-1beta and iNOS and nitric oxide (NO) production in a dose-dependent manner.
Hwang
Mi-Hyun
MH
Laboratory of Veterinary Pharmacology & Toxicology, College of Veterinary Medicine, Kyungpook National University, 702-701, Daegu, Republic of Korea.
Lim
Jong-Hwan
JH
Yun
Hyo-In
HI
Rhee
Man-Hee
MH
Cho
Jae-Youl
JY
Hsu
Walter H
WH
Park
Seung-Chun
SC
D000818:Animals; D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D055666:Lipopeptides; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis* / Q000235:genetics; D010456:Peptides, Cyclic / Q000737:chemistry / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
D001426:Bacterial Proteins; D007375:Interleukin-1; D055666:Lipopeptides; D008070:Lipopolysaccharides; D010456:Peptides, Cyclic; C009365:surfactin peptide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse