Association between pemphigus vulgaris and human leukocyte antigen in Han nation of northeast China.
OBJECTIVE
To investigate the relationship between pemphigus vulgaris (PV) and human leukocyte antigen (HLA) in Han nation of northeast China.


METHODS
Standard microcytotoxicity test and polymerase chain reaction-sequence specific primers method were used to detect the HLA class I antigens and HLA-DRB1 and DQB1 alleles in 27 patients with PV and results were compared with control group.


RESULTS
Gene and phenotype frequencies of HLA-A3, A26(10), B60(40), and B13 (27.99%, 48%; 16.11%, 30%; 23.02%, 41%; 16.11%, 30%, respectively) increased significantly in PV group compared with control (1.01%, 2%; 0.5%, 1%; 4.61%, 9%; 5.13%, 10%, respectively). After P value correction, the difference of A3, A26 (10), and B60 (40) between the two groups was still significant. The gene frequencies of HLA-DRB1*140x (1401, 1404, 1405, 1407, 1408), DRB1*120x, and DQB1*0503 alleles in PV group (42.26%, 25.46%, and 23.02%) were significantly higher than control group (5.09%, 7.74%, and 1.89%). After P value correction, the difference was still significant between the two groups.


CONCLUSIONS
PV significantly relates with HLA in PV patients of Han nation of northeast China.
Geng
Long
L
Department of Dermatology, First Affiliated Hospital, China Medical University, Shenyang 110001. gength@yahoo.com.cn
Wang
Yan
Y
Zhai
Ning
N
Lu
Ya-Ni
YN
Song
Fang-Ji
FJ
Chen
Hong-Duo
HD
D000328:Adult; D000368:Aged; D044466:Asian People / Q000208:ethnology; D002681:China / Q000208:ethnology; D005260:Female; D005787:Gene Frequency; D015234:HLA-A Antigens / Q000235:genetics*; D015791:HLA-A3 Antigen / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008297:Male; D008875:Middle Aged; D010392:Pemphigus / Q000235:genetics*; D010641:Phenotype
D015234:HLA-A Antigens; D015791:HLA-A3 Antigen; D015235:HLA-B Antigens; C081918:HLA-B60 antigen; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains