[Cytokines secretion by peripheral blood mononuclear cells from hepatitis C patients after stimulation with synthetic peptides at the highly variable region].
OBJECTIVE
Test the cytokines secretion by peripheral blood mononuclear cells (PBMC) from hepatitis C patients after stimulation with highly variable region (HVR1) synthetic peptides.


METHODS
ELISA was used to test the cytokines secretion, flow cytometry to group the proliferated cells, and the antigenicity of synthetic peptide was predicted by the computer modeling.


RESULTS
(1)There were PBMC proliferation when stimulated by HVR1 antigen synthetic peptides. (2) There was a rise of IFN-gamma, IL-4 and IL-10. But no rise of IL-2 and TNF-gamma was found. (3) The proliferated cells were mainly CD4+ lymphocytes.


CONCLUSIONS
PMBC from hepatitis C patients tend to secret Th2 cytokines after stimulation with HVR1 designed by the authors.
Chi
Shu-ping
SP
Department of Immunology, Institute of infectious diseases, The No. 302 Hospital of PLA, Beijing 100039, China.
Li
Jing
J
Gao
Rong
R
Su
Hai-bin
HB
Li
Bo-an
BA
Li
Wei
W
Cheng
Yun
Y
D000595:Amino Acid Sequence; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006526:Hepatitis C / Q000097:blood* / Q000821:virology; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors
D009842:Oligopeptides; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma