title: Potential of functional strains, isolated from traditional Maasai milk, as starters for the production of fermented milks.
abstract: The purpose of this research was the evaluation of technological features and of the ability of functional LAB strains with desirable sensory characteristics, to produce fermented milk. Eight strains of Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus paracasei and Lactococcus lactis, isolated from Maasai traditional fermented milk in Kenya and previously tested for their probiotic properties, were selected for this investigation. Technological features such as growth kinetics in fresh heat-treated whole milk medium and survival in the final product during storage at 4 degrees C, were studied. The strains Lb. acidophilus BFE 6,059, Lb. paracasei BFE 5,264 and Lc. lactis BFE 6,049 showed the best potential and were thus selected for use as starter cultures in further trials with the objective to improve their technological performance and to optimise the sensory features of fermented milk obtained. The effects of fat (F), non-fat milk solids (S) and fermentation temperature (T), modulated according to a Central Composite Design, on fermentation rates and viability losses during refrigerated storage of the chosen starters, and on product texture parameters, were studied. From the data analysis, it was possible to select optimum conditions for enhancing positive sensory traits of final products and for improving the survival of these potentially probiotic cultures.
authors.affiliation: University of Bologna, Dipartimento di Protezione e Valorizzazione Agroalimentare, via Fanin, 46, 40127 Bologna, Italy. francesca.patrignani@unibo.it
authors.forename: Francesca
authors.initials: F
authors.lastname: Patrignani
authors.forename: Rosalba
authors.initials: R
authors.lastname: Lanciotti
authors.forename: Julius Maina
authors.initials: JM
authors.lastname: Mathara
authors.forename: Maria Elisabetta
authors.initials: ME
authors.lastname: Guerzoni
authors.forename: Wilhelm H
authors.initials: WH
authors.lastname: Holzapfel
mesh_terms: D000818:Animals; D043302:Cultured Milk Products / Q000382:microbiology*; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D005524:Food Technology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007630:Kenya; D007700:Kinetics; D007778:Lactobacillus / Q000254:growth & development* / Q000302:isolation & purification; D019936:Probiotics; D012212:Rheology; D013649:Taste; D013696:Temperature; D013997:Time Factors