Polyamine oxidase and acrolein as novel biochemical markers for diagnosis of cerebral stroke.
OBJECTIVE
We found previously that plasma levels of acrolein (CH2=CHCHO) and spermine oxidase (SMO) were well correlated with the degree of severity of chronic renal failure. The aim of this study was to test whether the levels of these 2 markers and of acetylpolyamine oxidase (AcPAO) were increased in the plasma of stroke patients.


METHODS
The activity of AcPAO and SMO and the level of protein-conjugated acrolein in plasma of the stroke patients and normal subjects were measured by high-performance liquid chromatography and ELISA, respectively. Focal infarcts were estimated by MRI or computed tomography (CT).


RESULTS
The levels of AcPAO, SMO, and acrolein were significantly increased in the plasma of stroke patients. The size of stroke was nearly parallel with the multiplied value of acrolein and total polyamine oxidase (AcPAO plus SMO). After the onset of stroke, an increase in AcPAO first occurred, followed by increased levels of SMO and finally acrolein. In 1 case, an increase in AcPAO and SMO preceded focal damage as detected by MRI or CT. Furthermore, stroke was confirmed by MRI in a number of mildly symptomatic patients (11 cases) who had increased levels of total polyamine oxidase and acrolein. Among apparently normal subjects (8 cases) who had high values of acroleinxtotal polyamine oxidase, stroke was found in 4 cases by MRI.


CONCLUSIONS
The results indicate that increased levels of AcPAO, SMO, and acrolein are good markers of stroke.
Tomitori
Hideyuki
H
Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan.
Usui
Teruyoshi
T
Saeki
Naokatsu
N
Ueda
Shiro
S
Kase
Hiroshi
H
Nishimura
Kazuhiro
K
Kashiwagi
Keiko
K
Igarashi
Kazuei
K
D000171:Acrolein / Q000097:blood*; D000368:Aged; D015415:Biomarkers / Q000097:blood; D005188:False Negative Reactions; D005260:Female; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000097:blood; D008279:Magnetic Resonance Imaging; D008297:Male; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000097:blood*; D010865:Pilot Projects; D020521:Stroke / Q000097:blood* / Q000175:diagnosis*; D014057:Tomography, X-Ray Computed; D000097667:Polyamine Oxidase
D000171:Acrolein; D015415:Biomarkers; D008239:Lysine; D000587:Oxidoreductases Acting on CH-NH Group Donors; D000097667:Polyamine Oxidase; C113380:N(epsilon)-(3-formyl-3,4-dihydropiperidino)lysine