title: Clonal populations of chondrocytes with progenitor properties identified within human articular cartilage.
abstract: The aim of the present study was to identify and characterize progenitor properties of human articular chondrocytes selected by using agarose suspension culture. In this chondrogenic selective culture condition, about 3.6% of seeded surplus chondrocytes from patients undergoing articular chondrocyte transplantation proliferated and formed cell clusters after 6 weeks. Phase-contrast microscopy and transmission electron microscopy revealed four different types of cell clusters differing in cellular content and matrix production. Based on their morphological features, they were named the homogenous (H), the homogenous matrix (HM), the differentiated matrix (DM) and the differentiated (D) cell clusters. All cell clusters showed positive safranin O staining, and matrix was positive for antibodies detecting type II collagen and aggrecan. The clusters were further demonstrated to express the genes for fibroblast growth factor receptor 3, type IIA collagen and type IIB collagen, while type X collagen was not expressed. After subcloning, the H and HM clusters demonstrated the best proliferative capacity. Chondrocytes from these two cell clusters also showed phenotypic plasticity in chondrogenic, adipogenic as well as osteogenic assays. This study demonstrates that existing subpopulations of cells with chondroprogenitor properties can be isolated from human adult articular cartilage using agarose suspension cultures.
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Goteborg, Sweden. Maria.Thornemo@medic.gu.se
authors.forename: M
authors.initials: M
authors.lastname: Thornemo
authors.forename: T
authors.initials: T
authors.lastname: Tallheden
authors.forename: E
authors.initials: E
authors.lastname: Sjogren Jansson
authors.forename: A
authors.initials: A
authors.lastname: Larsson
authors.forename: K
authors.initials: K
authors.lastname: Lovstedt
authors.forename: U
authors.initials: U
authors.lastname: Nannmark
authors.forename: M
authors.initials: M
authors.lastname: Brittberg
authors.forename: A
authors.initials: A
authors.lastname: Lindahl
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers; D002358:Cartilage, Articular / Q000166:cytology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D010641:Phenotype; D013234:Stem Cells / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D015415:Biomarkers