Oxidized phospatidylcholine but not native phosphatidylcholine inhibits inducible nitric oxide synthase in RAW 264.7 macrophages.
This study was designed to compare the effects of oxidized 1-palmitoyl-2-arachidonyl-sn-glycero-3-phosphorylcholine (PAPC) and native PAPC on the inducible nitric oxide synthase (iNOS) in the macrophage cell line RAW 264.7. Macrophages stimulated by bacterial lipopolysaccharide (1 microg/ml) were incubated with increasing amounts of native or oxidized PAPC (oxPAPC, 10-20 microg/ml). Cells incubated with oxPAPC showed a dose-dependent inhibition of inducible nitric oxide synthesis, as well as reduced iNOS protein expression and mRNA levels. Additionally, chromatin immunoprecipitation assay revealed that oxPAPC reduced the interaction of the active NF-kappaB subunit p65 with the iNOS promoter region when compared to native PAPC.
Friedl
Roswitha
R
Medical University of Vienna, Institute of Physiology, Schwarzspanierstrasse 17, A-1090 Vienna, Austria. roswitha.friedl@univie.ac.at
Pichler
Ingeborg
I
Spieckermann
Paul
P
Moeslinger
Thomas
T
D000818:Animals; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors* / Q000235:genetics; D010084:Oxidation-Reduction; D010742:Phospholipid Ethers / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors* / Q000378:metabolism
C400184:1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphorylcholine; D008070:Lipopolysaccharides; D010742:Phospholipid Ethers; D051996:Transcription Factor RelA; D052247:Nitric Oxide Synthase Type II