Up-regulation of defense enzymes is responsible for low reactive oxygen species in malignant prostate cancer cells.
Reactive oxygen species (ROS) are involved in a diversity of important phenomena in the process of tumor development. To investigate the alterations of oxidative stress and their related systems in tumor progression, a variety of components in the antioxidative stress defense system were examined in prostate cancer cell lines, PC3 and LNCaP. Cell surface molecules involved in metastasis were expressed highly in PC3 cells compared with LNCaP cells, and strong invasion ability was shown in PC3 cells only. ROS level in LNCaP cells was twice higher than that in PC3 cells, although nitric oxide (NO) level was similar between the two cell lines. The content of GSH increased up to about 2-fold in PC3 compared with LNCaP. Activities of glutathione reductase, thioredoxin reductase, and glutathione S-transferase except catalase are significantly higher in PC3 cells than in LNCaP cells. Furthermore, oxidative stress-inducing agents caused down-regulation of GSH and glutathione S-transferase much more significantly in LNCaP cells than in PC3 cells. These results imply that malignant tumor cells may maintain low ROS content by preserving relatively high anti-oxidative capacity, even in the presence of stressful agents.
Lim
Hye Won
HW
Division of Life Sciences, Kangwon National University, Chuncheon 200-701, Korea.
Hong
Suntaek
S
Jin
Wook
W
Lim
Seunghwan
S
Kim
Su Jung
SJ
Kang
Hyun Jung
HJ
Park
Eun Hee
EH
Ahn
Kisup
K
Lim
Chang Jin
CJ
D000975:Antioxidants / Q000378:metabolism; D045744:Cell Line, Tumor; D004790:Enzyme Induction; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D013880:Thioredoxin-Disulfide Reductase