B7-H3: another molecule marker for Mo-DCs?
Using a newly generated monoclonal antibody (2E6) against human B7-H3, we explored the expression of the molecule on dendritic cells derived from monocytes (Mo-DCs). Its expression was examined by means of immunostaining and flow cytometric (FCM) analysis. The results showed that B7-H3 was expressed in the course of Mo-DC maturation induced with interleukin 4 (IL-4) and granulocyte/macrophage colony-stimulating factor (GM-CSF). The expression could be detected at all the stages of Mo-DC differentiation, and remained at a quite stable level. Interestingly, B7-H3 was not expressed by T cells and B cells, even these cells were activated respectively by PHA or PWM. A weak expression could be detected on resting monocytes. These data showed that constitutive expression of B7-H3 at a high level was found on imDCs and mDCs derived from monocytes. Due to no expression on T cells and B cells, we speculate that B7-H3 might be another valuable molecule marker for Mo-DCs.
Zhang
Guangbo
G
Clinical Immunology Laboratory of Jiangsu Province, Suzhou University No.1 Affiliated Hospital, Suzhou 215006, China.
Dong
Qiuming
Q
Xu
Ying
Y
Yu
Gehua
G
Zhang
Xueguang
X
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism; D060887:B7 Antigens; D018122:B7-1 Antigen / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism*; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D060887:B7 Antigens; D018122:B7-1 Antigen; C486038:Cd276 protein, mouse; D011994:Recombinant Proteins; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor