Interactions of microorganisms isolated from gilthead sea bream, Sparus aurata L., on Vibrio harveyi, a pathogen of farmed Senegalese sole, Solea senegalensis (Kaup).
Four bacterial isolates from farmed gilthead sea bream, Sparus aurata, included in a previous study as members of the Vibrionaceae and Pseudomonodaceae and the genus Micrococcus, have been evaluated for their adhesive ability to skin and intestinal mucus of farmed Senegalese sole, Solea senegalensis, and their antagonistic effect on Vibrio harveyi, a pathogen of sole. These isolates showed higher adhesion to sole mucus than the pathogenic strains of V. harveyi assayed. Only two of the isolates showed antagonistic activity to V. harveyi. Interactions of the four isolates with V. harveyi in respect of adhesion to skin and intestinal mucus under exclusion, competition and displacement conditions were studied. Three isolates were able to reduce the attachment to skin and intestinal sole mucus of a pathogenic strain of V. harveyi under displacement and exclusion conditions, but not under competition conditions. The in vivo probiotic potential of isolate Pdp11 was assessed by oral administration followed by challenge with the pathogenic V. harveyi strain Lg14/00. A group of 50 Senegalese sole received a commercial diet supplemented with 10(8) cfu g(-1) of lyophilized Lg14/00 for 15 days. A second group of fish received a non-supplemented commercial diet. After challenge the mortality of the fish receiving the diet supplemented with the potential probiotic isolate was significantly lower than that in the fish receiving the non-supplemented commercial diet. This study has shown that the ability to interfere with attachment of pathogens, as well as the adhesion to host surfaces, are suitable criteria for selection of candidate probiotics for use in the culture of Senegalese sole.
Chabrillón
M
M
Department of Microbiology, Faculty of Sciences, University of Malaga, Malaga, Spain.
Rico
R M
RM
Arijo
S
S
Díaz-Rosales
P
P
Balebona
M C
MC
Moriñigo
M A
MA
D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D017756:Aquaculture / Q000379:methods; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology; D018407:Bacterial Physiological Phenomena; D005393:Fish Diseases / Q000382:microbiology*; D005412:Flatfishes / Q000382:microbiology*; D007413:Intestinal Mucosa / Q000382:microbiology; D019936:Probiotics / Q000494:pharmacology* / Q000627:therapeutic use; D021541:Sea Bream / Q000382:microbiology*; D012867:Skin / Q000382:microbiology; D013045:Species Specificity; D014733:Vibrio / Q000187:drug effects / Q000472:pathogenicity*; D014735:Vibrio Infections / Q000188:drug therapy / Q000662:veterinary*