[The role of tissue factor in the invasion and metastasis of colorectal carcinoma cells].
OBJECTIVE
To investigate the role of tissue factor (TF) in the invasion and hematogenous metastasis of human colorectal carcinoma cells.


METHODS
The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into HT-29 and LoVo cells by the way of lipofactamine 2000. TF proteins in transfected cells were detected by Western Blot. Then the transfected and un-transfected tumor cells were implanted into nude mice (Balb/c Nu/Nu) to produce primary tumor, lung metastasis and liver metastasis respectively.


RESULTS
HT-29 and LoVo cells with sense-TFcDNA transfection showed increased TF expression compared with the cells without transfection, but the cells with antisense-TFcDNA transfection got the contrary change. The primary tumor growth and invasive range, lung metastasis and live metastasis all increased in sense transfectants but reduced in antisense transfectants.


CONCLUSIONS
TF can increase the invasion and hematogenous metastatic ability of human colorectal carcinoma cells.
Wan
Yuan-lian
YL
Department of General Surgery, First Hospital of Peking University, Beijing 100034, China.
Rong
Long
L
Liu
Yu-cun
YC
Yao
Hong-wei
HW
Wang
Xin
X
Wu
Tao
T
Pan
Yi-sheng
YS
Zhu
Jing
J
D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology*; D016373:DNA, Antisense / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D013925:Thromboplastin / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D014162:Transfection
D016373:DNA, Antisense; D018076:DNA, Complementary; D013925:Thromboplastin