Endosymbiotic Wolbachia of parasitic filarial nematodes as drug targets.
The parasitic nematodes Wuchereria bancrofti, Brugia malayi and B. timori cause a dreadful disease in humans known as lymphatic filariasis, which afflicts more than 120 million people worldwide. As per recent epidemiologic estimates on prevalence of W. bancrofti and B. malayi, about 428 million people are at risk, with 28 million microfilaria carriers and 21 million clinical cases spread out in 13 States and 5 Union Territories of India. The Indian subcontinent that comprises Bangladesh, India, Maldives, Nepal and Sri Lanka harbours 50 per cent of the world's lymphatic filarial disease burden. Recently, an endobacterium of Wolbachia species that belongs to the family Rickettsiaceae was found in all life cycle stages of these nematodes and the transmission is exclusively vertical through the embryonic stages of the female worms. People with filariasis have been exposed to these Wolbachia bacteria or their proteins by the natural killing of parasites. Wolbachia have also been identified occasionally in body fluids of infected patients. Evidence suggests that these Wolbachia are mutualistic symbionts and can be cured from the nematodes by several antibiotics having antirickettsial properties. Treatment of nematodes with tetracyclines affect Wolbachia and they get cleared from worm tissues; and this elimination causes reproductive abnormalities in worms and affect worm's embryogenesis, resulting in sterility. Although it is impractical, prolonged treatment with doxycycline significantly reduces the numbers of microfilaria in circulation, which is an important strategy to control transmission of filariasis by mosquito vectors. In this review, the current knowledge of Wolbachia as a drug target and potential ways to reduce the infection through anti-Wolbachia treatments is discussed.
Rao
Ramakrishna U
RU
Department of Internal Medicine, Infectious Diseases Division,Washington University School of Medicine, St. Louis, MO 63110, USA. rrao@wustl.edu
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004318:Doxycycline / Q000494:pharmacology* / Q000627:therapeutic use; D005368:Filariasis / Q000188:drug therapy* / Q000453:epidemiology*; D006801:Humans; D007194:India / Q000453:epidemiology; D012098:Reproduction / Q000187:drug effects; D013559:Symbiosis; D020577:Wolbachia / Q000187:drug effects*; D014958:Wuchereria bancrofti / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D004318:Doxycycline