title: Drosophila GPCR Han is a receptor for the circadian clock neuropeptide PDF.
abstract: The pigment-dispersing factor (PDF) is a neuropeptide controlling circadian behavioral rhythms in Drosophila, but its receptor is not yet known. From a large-scale temperature preference behavior screen in Drosophila, we isolated a P insertion mutant that preferred different temperatures during the day and night. This mutation, which we named han, reduced the transcript level of CG13758. We found that Han was expressed specifically in 13 pairs of circadian clock neurons in the adult brain. han null flies showed arrhythmic circadian behavior in constant darkness. The behavioral characteristics of han null mutants were similar to those of pdf null mutants. We also found that PDF binds specifically to S2 cells expressing Han, which results in the elevation of cAMP synthesis. Therefore, we herein propose that Han is a PDF receptor regulating circadian behavioral rhythm through coordination of activities of clock neurons.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science & Technology, 373-1 Guseong-Dong, Yuseong-Gu, Daejeon 305-701, Korea.
authors.forename: Seogang
authors.initials: S
authors.lastname: Hyun
authors.forename: Youngseok
authors.initials: Y
authors.lastname: Lee
authors.forename: Sung-Tae
authors.initials: ST
authors.lastname: Hong
authors.forename: Sunhoe
authors.initials: S
authors.lastname: Bang
authors.forename: Donggi
authors.initials: D
authors.lastname: Paik
authors.forename: Jongkyun
authors.initials: J
authors.lastname: Kang
authors.forename: Jinwhan
authors.initials: J
authors.lastname: Shin
authors.forename: Jaejung
authors.initials: J
authors.lastname: Lee
authors.forename: Keunhye
authors.initials: K
authors.lastname: Jeon
authors.forename: Seungyoon
authors.initials: S
authors.lastname: Hwang
authors.forename: Eunkyung
authors.initials: E
authors.lastname: Bae
authors.forename: Jaeseob
authors.initials: J
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001522:Behavior, Animal; D001667:Binding, Competitive; D015152:Blotting, Northern / Q000379:methods; D001921:Brain / Q000166:cytology; D002460:Cell Line; D002940:Circadian Rhythm / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D009043:Motor Activity / Q000502:physiology; D009154:Mutation; D009474:Neurons / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D010507:Periodicity; D011485:Protein Binding / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D013697:Thermosensing / Q000235:genetics / Q000502:physiology
chemical_list: D000906:Antibodies; D029721:Drosophila Proteins; D009479:Neuropeptides; C507073:PDFR protein, Drosophila; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C113883:pdf protein, Drosophila; D000242:Cyclic AMP