Fluoride dose response in pH-cycling models using bovine enamel.
The aim of this study was to establish methodologies for verification of the fluoride solution dose-response relationship using bovine enamel and pH-cycling models. Six models of the cariogenic challenge were performed, varying the time of demineralization and pH, time of remineralization, composition of de- and remineralization solutions, frequency and time of application of treatment solutions and pH-cycling duration. For the evaluation of the fluoride effect on caries dynamics, two proposed models provided for improvement in standardization of methods leading to a higher level of precision, demonstrating a dose response between treatments with regard to surface microhardness and DeltaZ. For the evaluation of the fluoride effect on enamel remineralization, the addition of fluoride to the de- and remineralization solutions and the reduction of frequency and time of application of fluoride solutions led to a more suitable pH-cycling model.
Vieira
Ana Elisa de Mello
AE
UNESP, São Paulo State University, School of Dentistry at Araçatuba, São Paulo, Brazil.
Delbem
Alberto Carlos Botazzo
AC
Sassaki
Kikue Takebayashi
KT
Rodrigues
Eliana
E
Cury
Jaime Aparecido
JA
Cunha
Robson Frederico
RF
D000704:Analysis of Variance; D000818:Animals; D002327:Cariostatic Agents / Q000008:administration & dosage*; D002417:Cattle; D003731:Dental Caries / Q000188:drug therapy; D003743:Dental Enamel / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005459:Fluorides / Q000008:administration & dosage*; D006244:Hardness / Q000187:drug effects; D018709:Statistics, Nonparametric; D014089:Tooth Remineralization
D002327:Cariostatic Agents; D005459:Fluorides