Novel transcription factor zfh-5 is negatively regulated by its own antisense RNA in mouse brain.
Here, we report features of a novel transcription factor zfh-5, which we isolated from the mouse brain; in addition to the mRNA, the antisense strand of zfh-5 is also expressed in the developing brain, in a manner complementary to the expression of zfh-5 mRNA. Although most neurons express zfh-5 mRNA soon after their final mitosis, several types of neurons, such as the pyramidal and granule cells in the hippocampus, express the zfh-5 antisense RNA prior to the mRNA expression. Using gene-targeting approach, we showed that this antisense RNA has a negative regulatory role on the expression of zfh-5 mRNA. These observations suggest that, in specific types of neurons, the expression of zfh-5 is additionally regulated by a mechanism depending on this antisense RNA.
Komine
Yuriko
Y
Division of Brain Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
Nakamura
Kenji
K
Katsuki
Motoya
M
Yamamori
Tetsuo
T
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000196:embryology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D016372:RNA, Antisense / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D016335:Zinc Fingers
D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D014157:Transcription Factors; C510895:ZFH-5 protein, mouse; D049452:Green Fluorescent Proteins