Detection of an mRNA polymorphism by differential display.
Differential display technology was utilized to compare programs of gene expression in primary cultures of human skin fibroblasts from normal volunteers and patients diagnosed with melancholic depression. Polymorphic transcripts of a single gene differing by one tandem repeat sequence of four nucleotides (TGAT) in the 3' noncoding region were detected.
Liang
Shan
S
Department of Psychiatry, Vanderbilt University Medical Center, Nashville, TN, USA.
Rossby
S Paul
SP
Liang
Peng
P
Shelton
Richard C
RC
Manier
D Hal
DH
Chakrabarti
Amitabha
A
Sulser
Fridolin
F
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D003866:Depressive Disorder / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012867:Skin / Q000166:cytology; D014158:Transcription, Genetic
D018076:DNA, Complementary; D009711:Nucleotides; D012333:RNA, Messenger; D012313:RNA; D004247:DNA