title: Metabolic activity of macrophages infected with hantavirus, an agent of hemorrhagic fever with renal syndrome.
abstract: Monocytes/macrophages are thought to play an important role in pathogenesis of viral infections. These cells are involved in distribution and persistence of viruses in the organism and also influence the regulation of immune reactions. The functional and enzymatic activities of macrophages infected with an agent of hemorrhagic fever with renal syndrome were analyzed for the first time. This disease is caused by a virus of the Hantavirus genus, the Bunyaviridae family. Activities of ectoenzymes 5 -nucleotidase and ATPase of the plasma membrane of the hantavirus-infected macrophages decreased along with the antigen accumulation in the infected cells. The contact of phagocytes with hantavirus resulted in activation in the cells of the oxygen-dependent metabolism and NO-synthase. The NO-synthase-dependent system of the infected macrophages was activated earlier than their oxygen-dependent system. The intracellular contents of acid and alkaline phosphatases increased within the first hours after the infection. The bactericidal activity of the hantavirus-infected macrophages relatively to Staphylococcus aureus increased during the specific antigen accumulation in the phagocytes. Thus, the infection of macrophages with hantavirus was associated with intracellular metabolic changes.
authors.affiliation: Institute of Epidemiology and Microbiology, Siberian Division of the Russian Academy of Medical Sciences, Vladivostok, 690087, Russia. pl_nat@hotmail.com
authors.forename: N G
authors.initials: NG
authors.lastname: Plekhova
authors.forename: L M
authors.initials: LM
authors.lastname: Somova
authors.forename: R A
authors.initials: RA
authors.lastname: Slonova
authors.forename: G G
authors.initials: GG
authors.lastname: Companets
authors.forename: V V
authors.initials: VV
authors.lastname: Luk'yanova
authors.forename: N V
authors.initials: NV
authors.lastname: Yakubovich
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D006476:Orthohantavirus / Q000472:pathogenicity*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology; D007962:Leukocytes / Q000378:metabolism / Q000821:virology; D008264:Macrophages / Q000378:metabolism / Q000821:virology*; D051379:Mice; D019001:Nitric Oxide Synthase / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism / Q000821:virology; D013997:Time Factors
chemical_list: D019001:Nitric Oxide Synthase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase