title: Identification and determination of major flavonoids in rat urine by HPLC-UV and HPLC-MS methods following oral administration of Dalbergia odorifera extract.
abstract: Flavonoids are the main active constituents of Dalbergia odorifera. The excretion of the major flavonoids in rat urine after oral administration of D. odorifera extract was investigated by HPLC-UV and HPLC-MS methods. Utilizing the HPLC-MS technique, 18 flavonoids, including five isoflavones, four isoflavanones, four neoflavones, two flavanones, two chalcones and one isoflavanonol were identified in free form in a urine sample based on the direct comparison of the corresponding tR, UV maximum absorbance (lambda(max)) values and MS data with the authentic standards. The amounts of the prominent flavonoids, (3R)-4'-methoxy-2',3,7-trihydroxyisoflavanone and vestitone, were determined by HPLC-UV with the internal standard method, and the validation procedure confirmed that it afforded reliable analysis of these two analytes in urine after oral administration of D. odorifera extract.
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100083, People's Republic of China.
authors.forename: Rongxia
authors.initials: R
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
authors.forename: Kaishun
authors.initials: K
authors.lastname: Bi
authors.forename: Dean
authors.initials: D
authors.lastname: Guo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002867:Chromones / Q000652:urine; D035782:Dalbergia / Q000737:chemistry*; D005419:Flavonoids / Q000652:urine*; D007529:Isoflavones / Q000652:urine; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: C509390:(3R)-4'-methoxy-2',3,7-trihydroxyisoflavanone; D002867:Chromones; D005419:Flavonoids; D007529:Isoflavones; D010936:Plant Extracts; C089075:vestitone