title: Antigene property of PNA conjugated to the nuclear localization signal peptide.
abstract: Peptide nucleic acid (PNA) is a DNA mimic with antigene properties. To enhance its capacity to enter in the cell and internalize in the nucleus, PNA has been conjugated to the nuclear localization signal (NLS) peptide, PKKKRKV PNA-NLS conjugates form stable hybrids with complementary DNA strands and poorly tolerate mismatched base pairing. Employed against cancer-associated genes, PNA-NLS exhibited a potent and specific antigene activity, suggesting exciting therapeutic approaches to cancer.
authors.affiliation: Department of Biochemical Science and Technology, School of Medicine, Udine, Italy.
authors.forename: Susanna
authors.initials: S
authors.lastname: Cogoi
authors.forename: Andrea
authors.initials: A
authors.lastname: Codognotto
authors.forename: Valentina
authors.initials: V
authors.lastname: Rapozzi
authors.forename: Luigi E
authors.initials: LE
authors.lastname: Xodo
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D011905:Genes, ras; D006801:Humans; D008957:Models, Genetic; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009154:Mutation; D009369:Neoplasms / Q000378:metabolism; D019913:Nuclear Localization Signals; D009691:Nucleic Acid Denaturation; D010179:Pancreas / Q000378:metabolism; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D018631:ras Proteins / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D019913:Nuclear Localization Signals; D020135:Peptide Nucleic Acids; D010455:Peptides; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D018631:ras Proteins