FISH screening of aneuploidies in preimplantation embryos to improve IVF outcome.
Preimplantation genetic diagnosis (PGD) has transformed the approach to the infertility patient in the IVF setting. Although the principal applications of PGD have been to prevent the transmission of sex-linked diseases, in time and with growing knowledge of the chromosomal abnormalities observed in preimplantation embryos, its applications have widened. Nowadays, apart from its implications in the prevention of transmission of chromosomal and genetic abnormalities, PGD is being used with increased frequency to improve the IVF outcome in patients with advanced maternal age (> or =38 years of age), recurrent miscarriage (> or =2 miscarriages), recurrent IVF failure (> or =3 failed IVF attempts) and severe male infertility. A high incidence of chromosomal abnormalities has been observed in these patient groups.
Rubio
Carmen
C
Instituto Valenciano de Infertilidad (IVI-Valencia), University of Valencia, Plaza Policía local No. 3, 46015 Valencia, Spain.
Rodrigo
Lorena
L
Pérez-Cano
Inmaculada
I
Mercader
Amparo
A
Mateu
Emilia
E
Buendía
Pilar
P
Remohí
José
J
Simón
Carlos
C
Pellicer
Antonio
A
D000026:Abortion, Habitual; D000328:Adult; D000782:Aneuploidy; D001706:Biopsy; D001755:Blastocyst / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000473:pathology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007246:Infertility; D007247:Infertility, Female / Q000175:diagnosis / Q000235:genetics; D007248:Infertility, Male / Q000175:diagnosis / Q000235:genetics; D008297:Male; D008423:Maternal Age; D008875:Middle Aged; D011247:Pregnancy; D011256:Pregnancy Outcome; D013997:Time Factors; D016896:Treatment Outcome