title: Hyper expression of kojibiose phosphorylase gene and trehalose phosphorylase gene from Thermoanaerobacter brockii ATCC35047 in Bacillus subtilis and selaginose synthesis utilizing two phosphorylases.
abstract: The kojibiose phosphorylase (KP) gene and trehalose phosphorylase (TP) gene from Thermoanaerobacter brockii ATCC35047 were intracellularly hyper-expressed under the control of the Bacillus amyloliquefaciens alpha-amylase promoter in Bacillus subtilis. The production yields were estimated to be 2.1 g of KP and 4.9 g of TP per liter of medium. Selaginose, non-reducing trisaccharide, was synthesized from trehalose utilizing the recombinant KP and TP from B. subtilis. Selaginose was not hydrolyzed by salivary amylase, artificial gastric juice, pancreatic amylase, or small intestinal enzymes.
authors.affiliation: Amase Institute, Hayashibara Biochemical Laboratories, Inc., 7-7 Amase Minami-machi, Okayama 700-0834, Japan. amaseken@hayashibara.co.jp
authors.forename: Takuo
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Mukai
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Maruta
authors.forename: Hikaru
authors.initials: H
authors.lastname: Watanabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamashita
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nishimoto
authors.forename: Michio
authors.initials: M
authors.lastname: Kubota
authors.forename: Hiroto
authors.initials: H
authors.lastname: Chaen
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D003001:Cloning, Molecular; D004187:Disaccharides / Q000378:metabolism; D015870:Gene Expression; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D006005:Phosphorylases / Q000235:genetics* / Q000378:metabolism; D046968:Thermoanaerobacter / Q000201:enzymology* / Q000235:genetics; D014312:Trisaccharides / Q000096:biosynthesis*
chemical_list: D004187:Disaccharides; D014312:Trisaccharides; C505610:selaginose; C008237:kojibiose; D005964:Glucosyltransferases; D006005:Phosphorylases; C023181:alpha,alpha-trehalose phosphorylase