The "resurrection method" for modification of specific proteins in higher plants.
We describe a new method designated "the resurrection method" by which a modified protein is expressed in higher plants in place of the original protein. The modified gene constructed by introducing synonymous codon substitutions throughout the original gene to prevent the sequence-specific degradation of its mRNA during RNA silencing is expressed while the expression of the original gene is suppressed. Here, we report the successful alteration of the biochemical properties of green fluorescent protein expressed in transgenic Nicotiana benthamiana, suggesting that this method could be useful for gene control in living plants.
Mori
Masashi
M
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi-machi, Ishikawa 921-8836, Japan. mori@ishikawa-pu.ac.jp
Dohi
Koji
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D020868:Gene Silencing; D018390:Gene Targeting / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D014176:Protein Biosynthesis; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D014026:Nicotiana / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D010940:Plant Proteins; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins