title: Identification of genes differentially expressed in wild type and Purkinje cell degeneration mice.
abstract: Purkinje cell degeneration (pcd) mice are characterized by death of virtually all cerebellar Purkinje cells by postnatal day 30. In this study, we used DNA microarray analysis to investigate differences in gene expression between the brains of wild type and pcd mice on postnatal day 20, before the appearance of clear-cut phenotypic abnormalities. We identified 300 differentially expressed genes, most of which were involved in metabolic and physiological processes. Among the differentially expressed genes were several calcium binding proteins including calbindin-28k, paravalbumin, matrix gamma-carboxyglutamate protein and synaptotagamins 1 and 13, suggesting the involvement of abnormal Ca2+ signaling in the pcd phenotype.
authors.affiliation: Department of Animal Biotechnology; Bio-Organ Research Center/Institute of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Korea.
authors.forename: Rui
authors.initials: R
authors.lastname: Xiao
authors.forename: Youngsook
authors.initials: Y
authors.lastname: Park
authors.forename: Vijaya R
authors.initials: VR
authors.lastname: Dirisala
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Sang June
authors.initials: SJ
authors.lastname: Um
authors.forename: Hoon Taek
authors.initials: HT
authors.lastname: Lee
authors.forename: Chankyu
authors.initials: C
authors.lastname: Park
mesh_terms: D000483:Alleles; D000818:Animals; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D051379:Mice; D009410:Nerve Degeneration / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011689:Purkinje Cells / Q000378:metabolism / Q000473:pathology* / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005819:Genetic Markers