title: Bovine tuberculosis in India: potential basis for zoonosis.
abstract: Our laboratory has designed a specific nested-PCR (N-PCR) assay, based on the hupB gene of Mycobacterium tuberculosis (Rv2986c) and Mycobacterium bovis (Mb3010c) as a method to differentiate these closely related species. The present paper deciphers the utility of this assay for identification of pathogenic Mycobacteria in clinical samples. Extra-pulmonary clinical samples obtained from cattle and humans were investigated. Pre-dominance of M. tuberculosis (15.7%) and M. bovis (26.8%) was seen in humans and cattle, respectively. However, more importantly, both mycobacterial pathogens (mixed infection) were identified in a number of samples. In humans 8.7% of the samples and 35.7% in cattle were classified as mixed infection. The detection of mixed infection with the mycobacterial pathogenic duo in humans and bovines denotes the prospect of potential transmission of these pathogens from humans to cattle (zoonosis) and vice versa (reverse zoonosis).
authors.affiliation: Department of Biotechnology, All India Institute of Medical Sciences, New Delhi-110029, India. hk_prasad@hotmail.com
authors.forename: H K
authors.initials: HK
authors.lastname: Prasad
authors.forename: A
authors.initials: A
authors.lastname: Singhal
authors.forename: A
authors.initials: A
authors.lastname: Mishra
authors.forename: N P
authors.initials: NP
authors.lastname: Shah
authors.forename: V M
authors.initials: VM
authors.lastname: Katoch
authors.forename: S S
authors.initials: SS
authors.lastname: Thakral
authors.forename: D V
authors.initials: DV
authors.lastname: Singh
authors.forename: S
authors.initials: S
authors.lastname: Chumber
authors.forename: S
authors.initials: S
authors.lastname: Bal
authors.forename: S
authors.initials: S
authors.lastname: Aggarwal
authors.forename: M V
authors.initials: MV
authors.lastname: Padma
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: M K
authors.initials: MK
authors.lastname: Singh
authors.forename: S K
authors.initials: SK
authors.lastname: Acharya
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D015373:Bacterial Typing Techniques; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D006657:Histones / Q000235:genetics; D006801:Humans; D007194:India; D009163:Mycobacterium bovis / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology*; D014380:Tuberculosis, Bovine / Q000175:diagnosis / Q000635:transmission*; D015047:Zoonoses
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006657:Histones; C510666:HupB protein, Mycobacterium tuberculosis