[The effects of resveratrol on the activation, proliferation and cytokine expression of murine T lymphocytes].
OBJECTIVE
To study the effects of resveratrol (RSV) on the activation, proliferation and cytokine expression of murine T lymphocytes.


METHODS
Murine T lymphocytes were isolated from lymph nodes and cultured in vitro. The lymphocytes were pre-treated with RSV for 1 h prior to activation with PDB plus ionomycin. (3)H-TdR incorporation was used to detect T cell proliferation. IL-2 and IFN-gamma mRNA were detected by RT-PCR, IL-2 and IFN-gamma proteins were detected by intracellular cytokine staining with flow cytometry.


RESULTS
RSV can inhibit the proliferation and expression of IL-2 and IFN-gamma of T lymphocytes.


CONCLUSIONS
The immunosuppressive action of RSV may be related with its inhibitory effects on T cell activation, proliferation and cytokine expression.
Yu
Yu
Y
Key Laboratory of Tissue Transplantation and Immunology of Ministry of Education, Jinan University, Guangzhou 510632, China.
Zeng
Yao-ying
YY
Ji
Yu-hua
YH
Zhao
Jing-xian
JX
Liu
Liang
L
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013267:Stilbenes / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D016207:Cytokines; D007376:Interleukin-2; D013267:Stilbenes; D015759:Ionomycin; D007371:Interferon-gamma; D000077185:Resveratrol