Single nucleotide polymorphism detection in promoter III of the acetyl-CoA carboxylase-alpha gene in sheep.
Three novel SNPs were identified in the locus OAR292286, encoding the DNA sequence of promoter III of the ovine acetyl-CoA carboxylase-alpha gene, in Italian sheep of four breeds: Gentile di Puglia (25 individuals) and Sopravissana (31) which are triple-purpose local endangered breeds, Comisana (25) which is a local non-selected, non-endangered dairy breed and Sarda (15) which is a popular selected high yielding dairy breed. Variant alleles are: G/T at 1330 bp, C/G at 1338 bp and C/T at 1430 bp. Frequencies of the variant alleles were calculated and chi-squared analysis of the differences in allele frequency between breed pairs was performed. Allele frequencies of the Sarda breed differ significantly from the other considered breeds.
Moioli
B
B
Istituto Sperimentale per la Zootecnia, Via Salaria, Monterotondo, Rome, Italy. bianca.moioli@isz.it
Napolitano
F
F
Orrù
L
L
Catillo
G
G
D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000818:Animals; D001947:Breeding / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D005787:Gene Frequency; D007558:Italy; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012756:Sheep / Q000235:genetics*
D017931:DNA Primers; D000103:Acetyl-CoA Carboxylase