Magnetic resonance tracking of dendritic cells in melanoma patients for monitoring of cellular therapy.
The success of cellular therapies will depend in part on accurate delivery of cells to target organs. In dendritic cell therapy, in particular, delivery and subsequent migration of cells to regional lymph nodes is essential for effective stimulation of the immune system. We show here that in vivo magnetic resonance tracking of magnetically labeled cells is feasible in humans for detecting very low numbers of dendritic cells in conjunction with detailed anatomical information. Autologous dendritic cells were labeled with a clinical superparamagnetic iron oxide formulation or (111)In-oxine and were co-injected intranodally in melanoma patients under ultrasound guidance. In contrast to scintigraphic imaging, magnetic resonance imaging (MRI) allowed assessment of the accuracy of dendritic cell delivery and of inter- and intra-nodal cell migration patterns. MRI cell tracking using iron oxides appears clinically safe and well suited to monitor cellular therapy in humans.
de Vries
I Jolanda M
IJ
Department of Tumor Immunology, Radboud University Nijmegen Medical Center and Nijmegen Center for Molecular Life Sciences, Geert Grooteplein 28, 6500 HB Nijmegen, The Netherlands.
Lesterhuis
W Joost
WJ
Barentsz
Jelle O
JO
Verdijk
Pauline
P
van Krieken
J Han
JH
Boerman
Otto C
OC
Oyen
Wim J G
WJ
Bonenkamp
Johannes J
JJ
Boezeman
Jan B
JB
Adema
Gosse J
GJ
Bulte
Jeff W M
JW
Scheenen
Tom W J
TW
Punt
Cornelis J A
CJ
Heerschap
Arend
A
Figdor
Carl G
CG
D002465:Cell Movement; D002469:Cell Separation; D017690:Cell Transplantation / Q000379:methods*; D003713:Dendritic Cells / Q000637:transplantation*; D005290:Ferric Compounds / Q000737:chemistry*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007167:Immunotherapy / Q000379:methods; D007205:Indium Radioisotopes / Q000627:therapeutic use; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008545:Melanoma / Q000628:therapy*; D015233:Models, Statistical; D009000:Monocytes / Q000378:metabolism; D010641:Phenotype; D011877:Radionuclide Imaging / Q000379:methods
D005290:Ferric Compounds; D007205:Indium Radioisotopes; C000499:ferric oxide