title: Cryptic polyadenylation of transcripts of an RNA virus gene introduced into tobacco plants.
abstract: We constructed an expression vector for the coat protein (CP) gene and the 3' untranslated region (3' UTR) of RNA virus (sweet potato feathery mottle virus severe strain (SPFMV-S)) lacking a foreign terminator. Out of seven transgenic tobacco plants, expression of the transgene was observed in six plants. RT-PCR analysis revealed that the transcripts had a poly(A) tail, and in most of them, polyadenylation occurred on the 5' side of the 3' UTR. These results suggest that the viral sequence contains a cryptic polyadenylation signal that permits 3'-end processing of the transcripts.
authors.affiliation: National Agricultural Research Center for Kyushu Okinawa Region, National Agriculture and Bio-oriented Research Organization, Kikuchi, Kumamoto, Japan. tkimura@affrc.go.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Kimura
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Kaoru
authors.initials: K
authors.lastname: Hanada
authors.forename: Susumu
authors.initials: S
authors.lastname: Takio
authors.forename: Akira
authors.initials: A
authors.lastname: Saito
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D026723:Polyadenylation; D012328:RNA Viruses / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D036022:Capsid Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; C091623:coat protein, sweet potato potyvirus