Interaction of porphyrin with G-quadruplex structures.
Isothermal titration calorimetry (ITC) is a sensitive technique for probing bimolecular processes and can provide direct information about the binding affinity and stoichiometry and the key thermodynamic parameters involved. ITC has been used to investigate the interaction of the ligand H2TMPyP to the two DNA quadruplexes, [d(AGGGT)]4 and [d(TGGGGT)]. Analysis of the ITC data reveals that porphyrin/quadruplex binding stoichiometry under saturating conditions is 1:2 for [d(AGGGT)]4 and 2:1 for [d(TGGGGT)], respectively.
Erra
Eva
E
Dipartimento di Chimica, Universitd di Napoli Federico II, Napoli, Italy.
Petraccone
Luigi
L
Esposito
Veronica
V
Randazzo
Antonio
A
Mayol
Luciano
L
Ladbury
John
J
Barone
Guido
G
Giancola
Concetta
C
D055592:Biophysical Phenomena; D001703:Biophysics; D002151:Calorimetry; D004247:DNA / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008024:Ligands; D008956:Models, Chemical; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes; D011166:Porphyrins / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors
D008024:Ligands; D009692:Nucleic Acid Heteroduplexes; D011166:Porphyrins; D004247:DNA