title: Survey of the year 2004 commercial optical biosensor literature.
abstract: The year 2004 represents a milestone for the biosensor research community: in this year, over 1000 articles were published describing experiments performed using commercially available systems. The 1038 papers we found represent an approximately 10% increase over the past year and demonstrate that the implementation of biosensors continues to expand at a healthy pace. We evaluated the data presented in each paper and compiled a 'top 10' list. These 10 articles, which we recommend every biosensor user reads, describe well-performed kinetic, equilibrium and qualitative/screening studies, provide comparisons between binding parameters obtained from different biosensor users, as well as from biosensor- and solution-based interaction analyses, and summarize the cutting-edge applications of the technology. We also re-iterate some of the experimental pitfalls that lead to sub-optimal data and over-interpreted results. We are hopeful that the biosensor community, by applying the hints we outline, will obtain data on a par with that presented in the 10 spotlighted articles. This will ensure that the scientific community at large can be confident in the data we report from optical biosensors.
authors.affiliation: Center for Biomolecular Interaction Analysis, University of Utah, Salt Lake City, UT 84132, USA.
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Rich
authors.forename: David G
authors.initials: DG
authors.lastname: Myszka
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D055095:Optics and Photonics; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D011506:Proteins