[The distribution and viability changes of the Agrobacteria in the vacuum infiltrated plants of Chinese cabbage].
To understand further the mechanism of agrobacteria transformation by vacuum infiltration, the distribution and viability changes of the agrobacteria in infiltrated Chinese cabbage( B. rapa ssp. chinesis) plants were investigated by histochemical staining and bacteria plating techniques. Results revealed that there were different quantities of Agrobacteria in different organs, among which the most quantity occurred in flowers, especially in ovules. The Agrobacteria lied in the space between cells and concentrated in the vascular bundle no matter the type of the organs examined. The CFUs (colony forming unit) of the Agrobacteria derived from the flowers were always the most in every experiment. The viabilities of the agrobacteria in the plants declined dramatically along with the elongation of the time after plant infiltration in all organs, but there were still 10(3) CFUs per gram fresh tissue in the flowers after 15 days of infiltration. These results may explain the fact that the ovules were the targets for the Agrobacteria mediated vacuum infiltration transformation.
Xu
Heng-Jian
HJ
College of Life Science , Shandong University of Technology, Zibo 255091, China. xhj310@163.com
Liu
Fan
F
Wang
Xiu-Feng
XF
Zhao
Hong
H
D001937:Brassica / Q000235:genetics* / Q000382:microbiology*; D015169:Colony Count, Microbial; D012231:Rhizobium / Q000502:physiology*; D013997:Time Factors; D014618:Vacuum