Glutathione reductase from human cataract lenses can be revived by reducing agents and by a molecular chaperone, alpha-crystallin.
OBJECTIVE
The aim of this study was to investigate how glutathione reductase (GR) loses its activity during cataract formation and whether it is possible to revive it back to the normal levels.


METHODS
In this study, endogenous as well as synthetic reducing systems (GSH, TTase, DTT, captopril) and alpha-crystallin at different concentrations were incubated with the soluble fraction of human cataract lens protein. The activity of glutathione reductase with or without the reducing agents and alpha-crystallin was tested, and the difference in activity gained was calculated.


RESULTS
Five agents (GSH, DTT, TTase, captopril, alpha-low crystallin) were able to revive the activity of GR from human cataract lenses to different extents.


CONCLUSIONS
This study shows that human lens GR activity was revived by different reducing agents as well as by a molecular chaperone (alpha-crystallin).
Rachdan
Diyaa
D
Nuffield Laboratory of Ophthalmology, University of Oxford, UK.
Lou
Marjorie F
MF
Harding
John J
JJ
D000818:Animals; D002216:Captopril / Q000494:pharmacology; D002386:Cataract / Q000201:enzymology*; D002417:Cattle; D004229:Dithiothreitol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D054477:Glutaredoxins; D005978:Glutathione / Q000494:pharmacology; D005980:Glutathione Reductase / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000201:enzymology*; D018832:Molecular Chaperones / Q000008:administration & dosage / Q000494:pharmacology*; D011490:Protein Disulfide Reductase (Glutathione) / Q000494:pharmacology; D019163:Reducing Agents / Q000008:administration & dosage / Q000494:pharmacology*; D038201:alpha-Crystallins / Q000008:administration & dosage / Q000494:pharmacology*
D054477:Glutaredoxins; D018832:Molecular Chaperones; D019163:Reducing Agents; D038201:alpha-Crystallins; D002216:Captopril; D005980:Glutathione Reductase; D011490:Protein Disulfide Reductase (Glutathione); D005978:Glutathione; D004229:Dithiothreitol