Biochemical properties and localization of the beta-expansin OsEXPB3 in rice (Oryza sativa L.).
Alpha-expansins are bound to the cell wall of plants and can be solubilized with an extraction buffer containing 1 M NaCl. Localization of alpha-expansins in the cell wall was confirmed by immunogold labeling and electron microscopy. The subcellular localization of vegetative beta-expansins has not yet been studied. Using antibodies specific for OsEXPB3, a vegetative beta-expansin of rice (Oryza sativa L.), we found that OsEXPB3 is tightly bound to the cell wall and, unlike alpha-expansins, cannot be solubilized with extraction buffer containing 1 M NaCl. OsEXPB3 protein could only be extracted with buffer containing SDS. The subcellular localization of the OsEXPB3 protein was confirmed by immunogold labeling and electron microscopy. Gold particles were mainly distributed over the primary cell walls. Immunohistochemistry showed that OsEXPB3 is present in all regions of the coleoptile and root tissues tested.
Lee
Yi
Y
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824-1312, USA.
Choi
Dongsu
D
D000906:Antibodies / Q000737:chemistry; D002473:Cell Wall / Q000201:enzymology / Q000378:metabolism; D018548:Cotyledon / Q000737:chemistry / Q000201:enzymology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D012275:Oryza / Q000737:chemistry* / Q000166:cytology / Q000276:immunology; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics; D018517:Plant Roots / Q000737:chemistry / Q000201:enzymology; D011485:Protein Binding; D013045:Species Specificity
D000906:Antibodies; D010940:Plant Proteins; C095825:expansin protein, plant