title: [Experimental study on induction of the normal human melanocytes apoptosis in vitro by cycloheximide and TNF-alpha].
abstract: OBJECTIVE
  To investigate the effects of cycloheximide and TNF-alpha on melanocyte (MC).
  
  
  METHODS
  Melanocyte apoptosis was studied with MTT, transmitting electron microscopy and fluorescence labeling of alive cells.
  
  
  RESULTS
  We added TNF-alpha and cycloheximide in melanocytes, and the typical apoptosis appeared 24 hours later, with chromatin condensation, nuclear pyknosis and apoptotic bodies formation. The results of cytometry showed the typical apoptotic peak.
  
  
  CONCLUSIONS
  TNF-alpha and cycloheximide together could inhibit MC proliferation and induce MC apoptosis.
authors.affiliation: Department of Oral and Maxillofacial Plastic Surgery, Stomatological College, Xi'an Jiao Tong University, Xi'an 710004, China.
authors.forename: Zhuang-Qun
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Tu
authors.forename: Zheng-Hui
authors.initials: ZH
authors.lastname: Wang
authors.forename: Xiao-Yi
authors.initials: XY
authors.lastname: Hu
authors.forename: Tie-Liang
authors.initials: TL
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation; D003513:Cycloheximide / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008544:Melanocytes / Q000166:cytology* / Q000187:drug effects*; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D014409:Tumor Necrosis Factor-alpha; D003513:Cycloheximide