Silyl protecting groups for oligonucleotide synthesis removed by a ZnBr2 treatment.
An oligonucleotide protected with N-(trimethylsilyloxycarbonyl) (Teoc) and P-(trimethylsilylethanol) (Tse) groups was synthesized and deprotected by a single ZnBr2 treatment. Finally it was released from the solid support by cleavage of a disulfide linkage with TCEP. The olgonucleotide was obtained without any basic treatment.
Ferreira
Fernando
F
Laboratoire de Chimie Organique Biomoléculaire de Synthèse, UMR 5625 CNRS-UM II, Université de Montpellier U1, Place E. Bataillon, 34095 Montpellier Cedex 5, France.
Morvan
François
F
D001965:Bromides / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003839:Deoxyadenosines / Q000737:chemistry; D003849:Deoxyguanosine / Q000737:chemistry; D008956:Models, Chemical; D008967:Molecular Biology / Q000379:methods*; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D010720:Phosphines / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D013997:Time Factors; D017967:Zinc Compounds / Q000737:chemistry*
D001965:Bromides; D003839:Deoxyadenosines; D003849:Deoxyguanosine; D009705:Nucleosides; D009711:Nucleotides; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D010710:Phosphates; D010720:Phosphines; D017967:Zinc Compounds; C080938:tris(2-carboxyethyl)phosphine; C093802:zinc bromide