[Effects of selenium and B-27 supplements on viability and differentiation of neural stem cell in newborn rat].
OBJECTIVE
To assess how trace element selenium and B27 supplements affect the neural stem cell (NSc) differentiation in vitro.


METHODS
The development and differentiation of NSc from the newborn rat were observed with primary culture and subculture during treating by sodium-selenite, and selenium-methyl-cysteine (SMC). The immunocytochemistry techniques were used to identify the NSc and mature protein expression with neuron marker beta-tubulin, astrocyte marker GFAP, and oligodendrocyte marker CNPase. The neurosphere morphology and neurite outgrowth were observed.


RESULTS
Adding the complete B-27 serum-free supplement, Selenium could promote the neurosphere viability, development and differentiation. Without selenium and B-27, neurosphere could not survive and differentiate. Without B-27 in the medium but there containing selenium, the neurosphere could promote the viability and development into neuron, astrocyte and oligodendrocyte, as compared with the no-containing B-27 and selenium groups, these differentiated cells might have more quantity, more branches and better morphological nerve net. The count of the neuron, astrocyte and oligodendrocyte was 11.2/Hp, 16.1/Hp and 9.3/Hp.


CONCLUSIONS
The selenium should be very important for neural stem cells' survival. Selenium could promote the neurosphere cells differentiation and development.
Tian
Dong-ping
DP
The Department of Pathology, Shantou University Medical College, Guangdong 515031, China.
Su
Min
M
Wu
Xian-ying
XY
Zhang
Ge
G
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D003545:Cysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016566:Organoselenium Compounds / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology*; D017279:Selenocysteine / Q000031:analogs & derivatives; D018038:Sodium Selenite / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D016895:Culture Media, Serum-Free; D005904:Glial Fibrillary Acidic Protein; D016566:Organoselenium Compounds; D014404:Tubulin; D017279:Selenocysteine; D012643:Selenium; D018038:Sodium Selenite; D003545:Cysteine; C002979:selenomethylselenocysteine