[Heme oxygenase-1 gene transfer protects rat kidney transplant from ischemia/reperfusion injury].
OBJECTIVE
To investigate the protective effect of Heme oxygenase-1 (HO-1) gene transfer on rat renal autograft against ischemia/reperfusion injury.


METHODS
HO-1 recombinant adenovirus vectors were constructed and transduced into rat renal autograft by renal arterial perfusion. The renal autografts were transplanted orthotopically after store at 4 degrees C for 24 h, followed by contralateral native nephrectomy 5 d after transplantation. There were 25 rats in the control group. 5 h and 3 d after transplantation, reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry were used to detect the expression of HO-1 gene; enzyme-labeled immunosorbent (ELISA) was used to measure HO-1 protein content in the homogenate of renal autograft.


RESULTS
The intensity of HO-1mRNA expression at 3 h and 3 d after transplantation were 0.65 +/- 0.11, 0.86 +/- 0.17 in the experimental group and 0.09 +/- 0.01, 0.15 +/- 0.02 in the control group respectively. The differences between the two groups were significant (t = 14.38, 11.73, P < 0.05). HO-1 protein content at 3 h and 3 d after transplantation were significantly increased in the experimental group, as compared with the control group [(297 +/- 61) ng/g and (468 +/- 51) ng/g versus (98 +/- 30) ng/g and (155 +/- 31) ng/g; t = 8.27, 14.83, P < 0.05]. HO-1 transduced autografts had less renal ischemic injury and lower serum creatinine level compared with control animals (P < 0.05).


CONCLUSIONS
Adenoviral vector can successfully transduce rat kidneys with the HO-1cDNA, which can protect rat renal autografts from ischemia/reperfusion injury.
Wu
Yu-dong
YD
Department of Urology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. wydkw@163.com
Wang
Gui-xian
GX
Wei
Jin-xing
JX
Wen
Xiao-gang
XG
Liu
Bing-qian
BQ
Wu
Chang-li
CL
Han
Rui-fa
RF
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D005260:Female; D005822:Genetic Vectors; D051547:Heme Oxygenase-1 / Q000096:biosynthesis / Q000235:genetics*; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism; D016030:Kidney Transplantation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D014162:Transfection; D014182:Transplantation, Autologous
D051547:Heme Oxygenase-1