title: Rcho-1 trophoblast stem cells: a model system for studying trophoblast cell differentiation.
abstract: The biology of trophoblast cell development can be investigated using in vitro model systems. The Rcho-1 trophoblast stem cell line was derived from a rat choriocarcinoma and is an effective tool for elucidating regulatory mechanisms controlling trophoblast cell differentiation. In this chapter, we describe methods used in the maintenance and manipulation of the Rcho-1 trophoblast cell line.
authors.affiliation: Institute of Maternal-Fetal Biology, University of Kansas Medical Center, Kansas City, USA.
authors.forename: Namita
authors.initials: N
authors.lastname: Sahgal
authors.forename: Lindsey N
authors.initials: LN
authors.lastname: Canham
authors.forename: Brent
authors.initials: B
authors.lastname: Canham
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Soares
mesh_terms: D000818:Animals; D015415:Biomarkers; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002469:Cell Separation; D002470:Cell Survival; D017690:Cell Transplantation; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D005615:Freezing; D011247:Pregnancy; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014162:Transfection; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
chemical_list: D015415:Biomarkers