title: CD14 and TLR4 gene polymorphisms in adult periodontitis.
abstract: Bacterial deposits, smoking, and host genetic factors play a major role in an individual's predisposition to periodontitis. Bacterial components are recognized by CD14 and toll-like receptor 4 (TLR4), resulting in a NF-kappaB-based inflammatory response. We hypothesized that functional CD14 and TLR4 polymorphisms contribute to periodontitis susceptibility. We aimed to investigate the occurrence of CD14-260C>T, TLR4 299Asp>Gly, and 399Thr>Ile gene polymorphisms in adult periodontititis. DNA was collected from 100 patients with severe periodontitis and from 99 periodontally healthy controls. The gene polymorphisms were determined by the PCR technique. The presence of the periodontal pathogens Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, and whether the subjects smoked, was included in the analyses. The CD14-260T/T genotype was found in 34.0% of periodontitis patients and in 20.2% of controls. Logistic regression analysis adjusted for gender, age, smoking, and prevalence of P. gingivalis and A. actinomycetemcomitans showed an association between the CD14-260T/T genotype and periodontitis (P = 0.004, OR 3.0, 95% CI 1.4-6.9). We conclude that the CD14-260T/T genotype contributes to the susceptibility to severe periodontitis in Dutch Caucasians.
authors.affiliation: Department of Periodontology, Section of Oral Microbiology, Academic Centre for Dentistry Amsterdam, Van der Boechorsstraat 7, 1081 BT Amsterdam, The Netherlands. ML.Laine@vumc.nl
authors.forename: M L
authors.initials: ML
authors.lastname: Laine
authors.forename: S A
authors.initials: SA
authors.lastname: Morré
authors.forename: L S
authors.initials: LS
authors.lastname: Murillo
authors.forename: A-J
authors.initials: AJ
authors.lastname: van Winkelhoff
authors.forename: A S
authors.initials: AS
authors.lastname: Peña
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D001224:Aspartic Acid / Q000235:genetics; D003596:Cytosine; D004198:Disease Susceptibility / Q000276:immunology; D005260:Female; D020022:Genetic Predisposition to Disease; D005998:Glycine / Q000235:genetics; D006801:Humans; D007532:Isoleucine / Q000235:genetics; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D011110:Polymorphism, Genetic / Q000235:genetics*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D012737:Sex Factors; D012907:Smoking; D013912:Threonine / Q000235:genetics; D013941:Thymine; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: D018950:Lipopolysaccharide Receptors; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D007532:Isoleucine; D013912:Threonine; D001224:Aspartic Acid; D003596:Cytosine; D013941:Thymine; D005998:Glycine