Growth and denitrifying activity of Xanthobacter autotrophicus CECT 7064 in the presence of selected pesticides.
The effects of the application of nine pesticides used commonly in agriculture (aldrin, lindane, dimetoate, methylparathion, methidation, atrazine, simazine, captan and diflubenzuron) on growth, CO2 production, denitrifying activity [as nitrous oxide (N2O) released] and nitrite accumulation in the culture medium by Xanthobacter autotrophicus strain CECT 7064 (Spanish Type Culture Collection) (a micro-organism isolated from a submerged fixed-film) were studied. The herbicide atrazine and the insecticide dimetoate totally inhibited growth and biological activity of X. autotrophicus at 10 mg l(-1), while the rest of the tested pesticides delayed the growth of strain CECT 7064 but did not drastically affect the bacterial growth after 96 h of culture. The denitrifying activity of X. autotrophicus was negatively affected by the pesticides application with the exception of fungicide captan. The release of N2O was strongly inhibited by several pesticides (aldrin, lindane, methylparathion, methidation and diflubenzuron), while dimetoate, atrazine and simazine inhibited totally the denitrifying activity of the strain. The effects of the pesticides on denitrifying submerged fixed-film reactor are discussed.
Sáez
Florentina
F
Group of Environmental Microbiology, Institute of Water Research, University of Granada, Granada, Spain.
Pozo
Clementina
C
Gómez
Miguel Angel
MA
Martínez-Toledo
Maria Victoria
MV
Rodelas
Belén
B
Gónzalez-López
Jesús
J
D002245:Carbon Dioxide / Q000378:metabolism; D009609:Nitrous Oxide / Q000378:metabolism*; D010575:Pesticides / Q000494:pharmacology*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D020584:Xanthobacter / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism
D010575:Pesticides; D014874:Water Pollutants, Chemical; D002245:Carbon Dioxide; D009609:Nitrous Oxide