Microfluidic biosensor based on an array of hydrogel-entrapped enzymes.
Here we show that a microfluidic sensor based on an array of hydrogel-entrapped enzymes can be used to simultaneously detect different concentrations of the same analyte (glucose) or multiple analytes (glucose and galactose) in real time. The concentration of paraoxon, an acetylcholine esterase inhibitor, can be quantified using the same approach. The hydrogel micropatch arrays and the microfluidic systems are easy to fabricate, and the hydrogels provide a convenient, biocompatible matrix for the enzymes. Isolation of the micropatches within different microfluidic channels eliminates the possibility of cross talk between enzymes.
Heo
Jinseok
J
Department of Chemistry, Texas A&M University, College Station, Texas 77842-3012, USA.
Crooks
Richard M
RM
D000110:Acetylcholinesterase / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D010261:Paraoxon / Q000378:metabolism; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D000110:Acetylcholinesterase; D005947:Glucose; D010261:Paraoxon; D005690:Galactose