title: Protection of mitochondrial integrity from oxidative stress by selenium-containing glutathione transferase.
abstract: The antioxidant activity of a novel artificial glutathione peroxidase-like enzyme, selenium-containing glutathione 5-transferase from Lucilia cuprina (seleno-LuGST1-1), was studied by using a ferrous sulfate/ascorbate-induced mitochondrial damage model system. Swelling of mitochondria, lipid peroxidation, and cytochrome-c oxidase activity were selected to evaluate the preservation of mitochondrial integrity in this system. Seleno-LuGST1-1 could effectively protect the mitochondria against oxidative damage in a dose-dependent manner and exhibited both higher catalytic activity and greater antioxidant ability than the classic mimic, 2-phenyl-1,2-benziososelenazol-3(2H)-one (Ebselen). This novel artificial biocatalyst therefore may have great potential for pharmacologic application in the treatment of reactive oxygen species-related diseases.
authors.affiliation: Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun, People's Republic of China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Yu
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Tianzhu
authors.initials: T
authors.lastname: Zang
authors.forename: Guimin
authors.initials: G
authors.lastname: Luo
authors.forename: Jiacong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D003576:Electron Transport Complex IV / Q000187:drug effects / Q000378:metabolism; D005982:Glutathione Transferase / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008929:Mitochondria, Heart / Q000187:drug effects*; D008933:Mitochondrial Swelling / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D051140:Selenoproteins / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D051140:Selenoproteins; D003576:Electron Transport Complex IV; D005982:Glutathione Transferase