Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat.
Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.
Jones
Huw D
HD
CPI Division, Rothamsted Research, Harpenden, AL5 2JQ, UK. huw.jones@bbsrc.ac.uk
Doherty
Angela
A
Wu
Huixia
H