Mercury tolerance of thermophilic Bacillus sp. and Ureibacillus sp.
Although resistance of microorganisms to Hg(II) salts has been widely investigated and resistant strains have been reported from many eubacterial genera, there are few reports of mercuric ion resistance in extremophilic microorganisms. Moderately thermophilic mercury resistant bacteria were selected by growth at 62 degrees C on Luria agar containing HgCl(2). Sequence analysis of 16S rRNA genes of two isolates showed the closest matches to be with Bacillus pallidus and Ureibacillus thermosphaericus. Minimum inhibitory concentration (MIC) values for HgCl(2) were 80 microg/ml and 30 microg/ml for these isolates, respectively, compared to 10 microg/ml for B. pallidus H12 DSM3670, a mercury-sensitive control. The best-characterised mercury-resistant Bacillus strain, B. cereus RC607, had an MIC of 60 microg/ml. The new isolates had negligible mercuric reductase activity but removed Hg from the medium by the formation of a black precipitate, identified as HgS by X-ray powder diffraction analysis. No volatile H(2)S was detected in the headspace of cultures in the absence or presence of Hg(2+), and it is suggested that a new mechanism of Hg tolerance, based on the production of non-volatile thiol species, may have potential for decontamination of solutions containing Hg(2+) without production of toxic volatile H(2)S.
Glendinning
K J
KJ
School of Biosciences, The University of Birmingham, B15 2TT, Edgbaston, Birmingham, UK.
Macaskie
L E
LE
Brown
N L
NL
D001407:Bacillus / Q000254:growth & development*; D024881:Drug Resistance, Bacterial / Q000502:physiology*; D006358:Hot Temperature; D006862:Hydrogen Sulfide / Q000378:metabolism; D008627:Mercuric Chloride / Q000378:metabolism*
D008627:Mercuric Chloride; D006862:Hydrogen Sulfide