Proteomic analysis of sera from hepatocellular carcinoma patients after radiofrequency ablation treatment.
Comparative proteomic analysis was used to search for characteristic alterations in the sera of hepatocellular carcinoma (HCC) patients who had undergone curative radiofrequency ablation treatment. Serum samples collected from eight patients before and after treatment were subjected to 2-DE. Eighty-eight protein spots differentially expressed with the treatment were selected by clustering analysis, and the proteins were identified by MS based on MALDI-TOF/TOF analysis and public database searches. The statistical analysis suggested that four proteins decreased after treatment (pro-apolipoprotein, alpha2-HS glycoprotein, apolipoprotein A-IV precursor, and PRO1708/PRO2044, which is the carboxy terminal fragment of albumin) and that seven proteins were increased after treatment, including leucine-rich alpha2-glycoprotein and alpha1-antitrypsin. These data facilitate the identification of differentially expressed proteins that are involved in HCC carcinogenesis and provide candidate biomarkers for the development of diagnostic and therapeutic tools.
Kawakami
Takayuki
T
Department of Gastroenterology, Graduate School of Medicine, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
Hoshida
Yujin
Y
Kanai
Fumihiko
F
Tanaka
Yasuo
Y
Tateishi
Keisuke
K
Ikenoue
Tsuneo
T
Obi
Shuntaro
S
Sato
Shinpei
S
Teratani
Takuma
T
Shiina
Shuichiro
S
Kawabe
Takao
T
Suzuki
Takamasa
T
Hatano
Naoya
N
Taniguchi
Hisaaki
H
Omata
Masao
M
D001054:Apolipoproteins A / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000601:surgery; D017115:Catheter Ablation; D015180:Electrophoresis, Gel, Two-Dimensional; D006023:Glycoproteins / Q000097:blood; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood* / Q000601:surgery; D011498:Protein Precursors / Q000097:blood; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D060749:alpha-2-HS-Glycoprotein
C556354:AHSG protein, human; D001054:Apolipoproteins A; D014408:Biomarkers, Tumor; D001798:Blood Proteins; D006023:Glycoproteins; C015246:LRG1 protein, human; D011498:Protein Precursors; D060749:alpha-2-HS-Glycoprotein; C022205:apolipoprotein A-IV