title: Systemic delivery of full-length C/EBP beta/liposome complex suppresses growth of human colon cancer in nude mice.
abstract: C/EBP beta (CCAAT/enhancer-binding protein beta) is an important transcription factor involved in cellular proliferation and differentiation. Overexpression of the full-length C/EBP beta protein results in cellular growth arrest and apoptosis. Using a nonviral liposome as carrier, we delivered the full-length C/EBP beta expression plasmid, pCN, into nude mice bearing CW-2 human colon cancer tumors via tail vein. Southern blots revealed that the major organs and tumors were transfected. Experimental gene therapy showed that a strong suppression of tumor growth was observed in the pCN-treated mice, and such suppression was due to the overexpression of C/EBP beta, leading to the increased apoptosis in tumors of pCN-treated mice. No apparent toxic effects of pCN/liposome complex were observed in the animals. Thus, C/EBP beta has tumor suppression effect in vivo and may be used in gene therapy for cancers.
authors.affiliation: State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Bei Bei
authors.initials: BB
authors.lastname: Fu
authors.forename: Ding Gan
authors.initials: DG
authors.lastname: Liu
mesh_terms: D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000032:analysis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000473:pathology / Q000628:therapy*; D004247:DNA / Q000032:analysis; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000633:toxicity; D006801:Humans; D007275:Injections, Intravenous; D008081:Liposomes / Q000008:administration & dosage / Q000633:toxicity; D051379:Mice; D008819:Mice, Nude
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D008081:Liposomes; D004247:DNA