Hyaline cartilage regeneration using mixed human chondrocytes and transforming growth factor-beta1- producing chondrocytes.
The purpose of this study was to investigate the efficacy of cartilage regeneration when using a mixture of transforming growth factor-beta1 (TGF-beta1)-producing human chondrocytes (hChon-TGF-beta1) and primary human chondrocytes (hChon) ("mixed cells"), compared with either hChon-TGF-beta1 or hChon cells alone. Specifically, mixed cells or hChon cells were first injected intradermally into the backs of immune-deficient nude mice to test the feasibility of cartilage formation in vivo. Both the mixed cells and the hChon-TGF-beta1 cells alone induced cartilage formation in nude mice, whereas hChon cells alone did not. To further test the efficacy of the cells in generating cartilage, an artificially induced partial thickness defect of the femoral condyle of a rabbit knee joint was loaded with hChon-TGF-beta1 cells with or without mixing additional untransduced hChon cells, and hyaline cartilage regeneration was observed at 4 or 6 weeks. The efficiency of complete filling of the defect and the quality of tissue generated after implanting were evaluated on the basis of a histological grading system modified from O'Driscoll et al. (J. Bone Joint Surg. 70A, 595, 1988). Significantly, mixed cells (14.2 +/- 0.9) produced significantly better results than hChon-TGF-beta1 (9.0 +/- 1.7) or hChon (8.0 +/- 1.8) cells alone. Histological and immunohistochemical staining of the newly repaired tissues produced after treatment with either mixed cells or hChon-TGF-beta1 cells alone showed hyaline cartilage- like characteristics. These results suggest that the implantation of mixed cells may be a clinically efficient method of regenerating hyaline articular cartilage.
Song
Sun U
SU
Clinical Research Center, College of Medicine, Inha University, Inchon, South Korea.
Cha
Young-Deog
YD
Han
Jeoung-Uk
JU
Oh
In-Suk
IS
Choi
Kyoung Baek
KB
Yi
Youngsuk
Y
Hyun
Jong-Pil
JP
Lee
Hyeon-Youl
HY
Chi
Guang Fan
GF
Lim
Chae-Lyul
CL
Ganjei
J Kelly
JK
Noh
Moon-Jong
MJ
Kim
Seong-Jin
SJ
Lee
Dug Keun
DK
Lee
Kwan Hee
KH
D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism / Q000502:physiology* / Q000637:transplantation; D020219:Chondrogenesis; D024043:Collagen Type II / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005269:Femur / Q000293:injuries; D005822:Genetic Vectors; D006652:Histological Techniques; D006801:Humans; D051457:Hyaline Cartilage / Q000293:injuries / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D007279:Injections, Subcutaneous; D051379:Mice; D008819:Mice, Nude; D010957:Plasmids; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D053773:Transforming Growth Factor beta1; D014183:Transplantation, Heterologous
D024043:Collagen Type II; D011994:Recombinant Proteins; C508960:TGFB1 protein, human; C508961:Tgfb1 protein, mouse; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D004247:DNA