title: Quantitative trait loci for hip dysplasia in a cross-breed canine pedigree.
abstract: Canine hip dysplasia is a common developmental inherited trait characterized by hip laxity, subluxation or incongruity of the femoral head and acetabulum in affected hips. The inheritance pattern is complex and the mutations contributing to trait expression are unknown. In the study reported here, 240 microsatellite markers distributed in 38 autosomes and the X chromosome were genotyped on 152 dogs from three generations of a crossbred pedigree based on trait-free Greyhound and dysplastic Labrador Retriever founders. Interval mapping was undertaken to map the QTL underlying the quantitative dysplastic traits of maximum passive hip laxity (the distraction index), the dorsolateral subluxation score, and the Norberg angle. Permutation testing was used to derive the chromosome-wide level of significance at p<0.05 for each QTL. Chromosomes 4, 9, 10, 11 (p<0.01), 16, 20, 22, 25, 29 (p<0.01), 30, 35, and 37 harbor putative QTL for one or more traits. Successful detection of QTL was due to the cross-breed pedigree, multiple-trait measurements, control of environmental background, and marked advancement in canine mapping tools.
authors.affiliation: Department of Clinical Sciences, Box 32 College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA. rjt2@cornell.edu
authors.forename: Rory J
authors.initials: RJ
authors.lastname: Todhunter
authors.forename: Raluca
authors.initials: R
authors.lastname: Mateescu
authors.forename: George
authors.initials: G
authors.lastname: Lust
authors.forename: Nancy I
authors.initials: NI
authors.lastname: Burton-Wurster
authors.forename: Nathan L
authors.initials: NL
authors.lastname: Dykes
authors.forename: Stuart P
authors.initials: SP
authors.lastname: Bliss
authors.forename: Alma J
authors.initials: AJ
authors.lastname: Williams
authors.forename: Margaret
authors.initials: M
authors.lastname: Vernier-Singer
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Corey
authors.forename: Carlos
authors.initials: C
authors.lastname: Harjes
authors.forename: Richard L
authors.initials: RL
authors.lastname: Quaas
authors.forename: Zhiwu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Robert O
authors.initials: RO
authors.lastname: Gilbert
authors.forename: Dietrich
authors.initials: D
authors.lastname: Volkman
authors.forename: George
authors.initials: G
authors.lastname: Casella
authors.forename: Rongling
authors.initials: R
authors.lastname: Wu
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Acland
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004285:Dogs; D005828:Genetics, Population; D005838:Genotype; D006619:Hip Dysplasia, Canine / Q000000981:diagnostic imaging / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D010375:Pedigree; D010641:Phenotype; D040641:Quantitative Trait Loci; D011859:Radiography; D012044:Regression Analysis; D013045:Species Specificity