Distribution of tccP in clinical enterohemorrhagic and enteropathogenic Escherichia coli isolates.
Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) are diarrheagenic pathogens that colonize the gut through the formation of attaching and effacing lesions, which depend on the translocation of effector proteins via a locus of enterocyte effacement-encoded type III secretion system. Recently, two effector proteins, EspJ and TccP, which are encoded by adjacent genes on prophage CP-933U in EHEC O157:H7, have been identified. TccP consists of a unique N-terminus region and several proline-rich domains. In this project we determined the distribution of tccP in O157:H7, in non-O157 EHEC, and in typical and atypical EPEC isolates. All the EHEC O157:H7 strains tested were tccP(+). Unexpectedly, tccP was also found in non-O157 EHEC, and in typical and atypical EPEC isolates, particularly in strains belonging to serogroups O26 (EHEC), O119 (typical EPEC), and O55 (atypical EPEC). We recorded some variation in the length of tccP, which reflects diversity in the number of the proline-rich repeats. These results show the existence of a class of "attaching and effacing" pathogens which express a combination of EPEC and EHEC virulence determinants.
Garmendia
Junkal
J
Centre for Molecular Microbiology and Infection, Flowers Building, Division of Molecular and Cellular Biology, Imperial College London, London SW7 2AZ, United Kingdom.
Ren
Zhihong
Z
Tennant
Sharon
S
Midolli Viera
Monica Aparecida
MA
Chong
Yuwen
Y
Whale
Andrew
A
Azzopardi
Kristy
K
Dahan
Sivan
S
Sircili
Marcelo Palma
MP
Franzolin
Marcia Regina
MR
Trabulsi
Luiz R
LR
Phillips
Alan
A
Gomes
Tânia A T
TA
Xu
Jianguo
J
Robins-Browne
Roy
R
Frankel
Gad
G
D000818:Animals; D001315:Australia; D001938:Brazil; D002681:China; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000662:veterinary; D019453:Escherichia coli O157 / Q000737:chemistry / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics*; D005516:Food Microbiology; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D006113:United Kingdom
D029968:Escherichia coli Proteins; C497366:EspJ protein, E coli; C505370:tccP protein, E coli