Modulation of gastric hemorrhage and ulceration by oxidative stress and histamine release in Salmonella typhimurium-infected rats.
Infection with Salmonella typhimurium can produce multiple organ dysfunctions. However, document concerning with gastric hemorrhagic ulcers occur in this infectious disease is lacking. The aim was to study modulation of gastric hemorrhagic ulcer by oxidative stress and mast cell histamine in S. typhimurium-infected rats. Additionally, the protective effects of drugs, such as ofloxacin, lysozyme chloride, ketotifen, ranitidine, and several antioxidants, including exogenous glutathione (GSH), allopurinol and dimethylsulfoxide (DMSO) were evaluated. Male Wistar rats were injected intrajejunally with a live culture of S. typhimurium (1 x 10(10) colony-forming units/rat) and followed by deprivation of food for 36 h. Age-matched control rats received sterilized vehicle only. Rat stomachs were irrigated for 3 h with either normal saline or a simulated gastric juice containing 100 mM HCl, 17.4 mM pepsin and 54 mM NaCl. S. typhimurium caused aggravation of offensive factors, including enhancing gastric acid back-diffusion, mucosal lipid peroxide generation, histamine release, microvascular permeability and hemorrhagic ulcer, as well as an attenuation of defensive substances, such as mucosal GSH and mucus level. Intragastric irrigation of gastric juice caused further aggravation of these gastric biochemical parameters. This exacerbation of ulcerogenic factors was abolished by pretreatment of ofloxacin and lysozyme chloride. Antioxidants, such as reduced GSH, allopurinol and DMSO also produced significant (P < 0.05) amelioration of gastric damage in S. typhimurium infected rats. In conclusion, gastric oxidative stress and histamine play pivotal roles in the formation of hemorrhagic ulcers that were effectively ameliorated by ofloxacin, lysozyme chloride, ketotifen, ranitidine, diamine oxidase and various antioxidants in S. typhimurium-infected rats.
Hung
Chen-Road
CR
Department of Pharmacology, College of Medicine, National Cheng-Kung University, Tainan 70101, Taiwan. crhung@mail.ncku.edu.tw
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004195:Disease Models, Animal; D005750:Gastric Juice / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000382:microbiology / Q000648:ultrastructure; D006471:Gastrointestinal Hemorrhage / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D005978:Glutathione / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects* / Q000502:physiology; D007213:Indomethacin / Q000494:pharmacology; D008054:Lipid Peroxides / Q000037:antagonists & inhibitors / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D009113:Muramidase / Q000037:antagonists & inhibitors / Q000494:pharmacology; D015242:Ofloxacin / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012481:Salmonella Infections, Animal / Q000150:complications* / Q000382:microbiology / Q000628:therapy; D012965:Sodium Chloride / Q000008:administration & dosage; D013276:Stomach Ulcer / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D007507:Therapeutic Irrigation / Q000379:methods
D000975:Antioxidants; D008054:Lipid Peroxides; D012965:Sodium Chloride; D000493:Allopurinol; D015242:Ofloxacin; D009113:Muramidase; D005978:Glutathione; D007213:Indomethacin; D004121:Dimethyl Sulfoxide