title: SNP detection and genetic mapping of porcine genes encoding enzymes in hepatic metabolic pathways and evaluation of linkage with carcass traits.
abstract: We have previously identified and mapped porcine expressed sequence tags (ESTs) derived from genes that are preferentially expressed in liver. The aim of the present study was to identify single nucleotide polymorphisms (SNPs) in porcine genes encoding enzymes in hepatic metabolic pathways and use the SNPs for mapping. Furthermore, these genes, which are involved in utilization and partitioning of nutrients, were examined for their effects on carcass and meat quality traits by linkage analyses. In total, 100 ESTs were screened for SNPs by single strand conformation polymorphism analyses across a diverse panel of animals with a 36% success rate. Twelve of 36 polymorphic loci segregated in a three-generation Duroc x Berlin Miniature Pig (F2) resource population, the DUMI resource population, and were genetically mapped. Interval mapping of the corresponding chromosomes was performed to verify mapping of the genes within quantitative trait loci (QTL) regions detected in this resource population. QTL with genome-wide significance were detected in the vicinity of GNMT, ESTL147 and HGD. These loci therefore are positional candidate genes.
authors.affiliation: Institute of Animal Breeding and Genetics, University of Bonn, 53115 Bonn, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Ponsuksili
authors.forename: S
authors.initials: S
authors.lastname: Chomdej
authors.forename: E
authors.initials: E
authors.lastname: Murani
authors.forename: U
authors.initials: U
authors.lastname: Bläser
authors.forename: H-J
authors.initials: HJ
authors.lastname: Schreinemachers
authors.forename: K
authors.initials: K
authors.lastname: Schellander
authors.forename: K
authors.initials: K
authors.lastname: Wimmers
mesh_terms: D000818:Animals; D001824:Body Constitution / Q000502:physiology*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004798:Enzymes / Q000235:genetics*; D020224:Expressed Sequence Tags; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008460:Meat; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D004798:Enzymes