title: PstIAFLP based markers for leaf rust resistance genes in common wheat.
abstract: The aim of the present study was to detect candidate DNA markers for selected leaf rust resistance genes. A total number of 286 loci in the 'Thatcher' near-isogenic lines carrying resistance gene Lr1, Lr9, Lr10, Lr13, Lr19, Lr21, Lr24, Lr26, Lr28, Lr35, and Lr37 were screened for DNA polymorphism by the PstIAFLP method. A survey with 33 selective primers yielded 16 candidate markers. Further validation studies on cultivars characterized for the presence and absence of selected resistance genes confirmed specificity of markers for Lr24, Lr26 and Lr37. The AFLP-based marker P42-530 was successfully converted into an STS marker. The new marker was linked with the Lr37-specific marker (CslVrga13) at the distance of 1.7 cM. The PstIAFLP method was found to be effective in the identification of DNA changes induced in hexaploid wheat by translocations from Agropyron elongatum, Secale cereale and Aegilops ventricosa.
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznań, Poland. lgol@igr.poznan.pl
authors.forename: Lidia
authors.initials: L
authors.lastname: Blaszczyk
authors.forename: Mirosław
authors.initials: M
authors.lastname: Tyrka
authors.forename: Jerzy
authors.initials: J
authors.lastname: Chełkowski
mesh_terms: D001483:Base Sequence; D001487:Basidiomycota; D002874:Chromosome Mapping; D019295:Computational Biology; D017931:DNA Primers; D005819:Genetic Markers / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers