[Comparative study on the resting habit of Anopheles minimus A and Anopheles minimus C in Yunnan Province].
OBJECTIVE
To compare the difference of resting habitat between Anopheles minimus A and An. minimus C in Yunnan Province.


METHODS
A village was selected in Mengla and Yuanjiang County respectively to collect mosquitoes by hanging ultraviolet lamps inside the cattle pen and human dwellings. The samples were identified by multiplex-PCR after morphological examination. Suspicious An. minimus A/C hybrid samples were identified by PCR-ASA, PCR-RFLP and D3 region sequence.


RESULTS
In human dwellings, the proportion of An. minimus A and C was 21.35% and 78.65%, and in cattle pen, 28.54% and 71.46% respectively. As for the An. minimus A/C hybrid, the result of PCR-ASA and PCR-RFLP displayed the pattern of both An. minimus A and An. minimus C (PCR-ASA: 376, 294 and 112 bp, PCR-RFLP:108, 268 and 376 bp), the D3 sequence displayed heterozygosis in the variation place of An. minimus A and An. minimus C, which means that the hybrid possesses the signal of both An. minimus A and An. minimus C.


CONCLUSIONS
There is no resting habit difference between An. minimus A and An. minimus C in Yunnan Province. In Mengla County, 5 An. minimus A/C hybrids have been found for the first time.
Zheng
Bin
B
National Institute of Parasitic Diseases, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
Tang
Lin-Hua
LH
Ma
Ya-Jun
YJ
Shi
Wen-Qi
WQ
Zhou
Shui-Sen
SS
Wang
Xue-Zhong
XZ
D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D002681:China; D006579:Heterozygote; D016133:Polymerase Chain Reaction; D013045:Species Specificity