Association of CAT polymorphisms with catalase activity and exposure to environmental oxidative stimuli.
We tested the hypotheses that catalase activity is modified by CAT single nucleotide polymorphisms (SNPs) (-262;-844), and by their interactions with oxidant exposures (coal dusts, smoking), lymphotoxin alpha (LTA, NcoI) and tumor necrosis factor (TNF, -308) in 196 miners. Erythrocyte catalase, superoxide dismutase, and glutathione peroxidase activities were measured. The CAT -262 SNP was related to lower catalase activity (104, 87 and 72 k/g hemoglobin for CC, CT and TT, respectively, p < 0.0001). Regardless of CAT SNPs, the LTA NcoI but not the TNF-308 SNP was associated with catalase activity (p = 0.04 and p = 0.8). CAT -262 T carriers were less frequent in highly exposed miners (OR = 0.39 [0.20-0.78], p = 0.007). In CAT -262 T carriers only, catalase activity decreased with high dust exposure (p = 0.01). Haplotype analyses (combined CAT SNPs) confirm these results. Results show that CAT -262 and LTA NcoI SNPs, and interaction with coal dust exposure, influenced catalase activity.
Nadif
Rachel
R
INSERM U472-IFR69, Villejuif, France. nadif@vjf.inserm.fr
Mintz
Margaret
M
Jedlicka
Anne
A
Bertrand
Jean-Pierre
JP
Kleeberger
Steven R
SR
Kauffmann
Francine
F
D000328:Adult; D002374:Catalase / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D003031:Coal / Q000633:toxicity*; D003032:Coal Mining; D004391:Dust; D004785:Environmental Pollutants / Q000633:toxicity*; D004912:Erythrocytes / Q000201:enzymology; D005838:Genotype; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008233:Lymphotoxin-alpha / Q000502:physiology; D008875:Middle Aged; D016877:Oxidants / Q000633:toxicity*; D010084:Oxidation-Reduction; D020641:Polymorphism, Single Nucleotide; D012906:Smoke / Q000009:adverse effects*; D013482:Superoxide Dismutase / Q000097:blood; D014026:Nicotiana / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D003031:Coal; D004391:Dust; D004785:Environmental Pollutants; D008233:Lymphotoxin-alpha; D016877:Oxidants; D012906:Smoke; D014409:Tumor Necrosis Factor-alpha; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase