[Construction, genetic stability and protective efficacy of recombinant fowlpox virus expressing hemagglutinin gene of H5N1 subtype avian influenza virus].
Construction, genetic stability and protective efficacy of a recombinant fowlpox virus expressing hemagglutinin (HA) gene (rFPV-HA) of H5 subtype avian influenza virus were conducted. The results indicated that the recombinant fowlpox virus was obtained and purified by blue plaque selection. HA expressed by rFPV-HA was identified by indirect immunofluorescence array. The rFPV-HA could express reporter gene LacZ and HA gene after fifteen successive passages in chicken embryo fibroblast. The rFPV-HA induced completely protection of chickens against lethal challenge with homologous avian influenza virus. The results show that rFPV-HA has good genetic stability and antigenicity and should be a useful tool in HPAI control program by preventing illness and death in chickens when the disease spreads widely.
Jia
Lijun
L
Animal Science and Veterinary Medicine college, Yangzhou University, Yangzhou 225009, China. jialijun2002@yahoo.com.cn
Peng
Daxin
D
Zhang
Yanmei
Y
Liu
Hongqi
H
Liu
Xiufan
X
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002642:Chick Embryo; D002645:Chickens; D005587:Fowlpox virus / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005818:Genetic Engineering; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D005585:Influenza in Birds / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D011897:Random Allocation; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D011994:Recombinant Proteins