title: Enzyme-electropolymer-based amperometric biosensors: an innovative platform for time-temperature integrators.
abstract: A novel exogenous time-temperature integrator (TTI) based on an amperometric glucose oxidase biosensor is presented. The TTI consists of the enzyme entrapped within an electrochemically generated poly(o-phenylenediamine) (PoPD) thin film deposited on the interior wall of a platinum (Pt) or a platinized stainless steel (Pt-SS) capsule. After thermal treatment, the TTI is mounted in a continuous flow system and connected to a potentiostat for amperometric detection of residual enzyme activity. A measurement is completed within 10 min. Isothermal treatments were carried out between 70 and 79.7 degrees C. Thermal inactivation of the immobilized enzyme followed apparent first-order kinetics with z values of 6.2 +/- 0.6 and 6.6 +/- 0.8 degrees C for Pt and Pt-SS capsules, respectively. These z values suggest that the proposed TTIs have the potential to assess pasteurization processes that target microorganism such as Listeria monocytogenes and Escherichia coli O157:H7.
authors.affiliation: Department of Food Science and Human Nutrition, Washington State University, Pullman, Washington 99164-6376, USA.
authors.forename: José I
authors.initials: JI
authors.lastname: Reyes-De-Corcuera
authors.forename: Ralph P
authors.initials: RP
authors.lastname: Cavalieri
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Powers
authors.forename: Juming
authors.initials: J
authors.lastname: Tang
authors.forename: Dong H
authors.initials: DH
authors.lastname: Kang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D005516:Food Microbiology; D005949:Glucose Oxidase; D011108:Polymers; D011199:Potentiometry / Q000295:instrumentation; D013696:Temperature; D013995:Time
chemical_list: D011108:Polymers; D005949:Glucose Oxidase