Characterization of prostaglandin E1 transport by rat renal cortical slices.
The purpose of this study was to examine prostaglandin E1 (PGE1) transport in rat kidney. [3H]PGE1 administered intravenously was accumulated most abundantly in the renal cortex. Infusion of probenecid and bromcresol green (BCG) decreased [3H]PGE1 accumulation in the renal cortex after injection of [3H]PGE1. To further investigate PGE1 transport in the kidney, [3H]PGE1 uptake by renal cortical slices was examined. Probenecid and BCG inhibited [3H]PGE1 uptake by the slices. Unlabeled PGE1 decreased [3H]PGE1 uptake by renal cortical slices in a concentration-dependent manner. The inhibitory effect of various dicarboxylates with different carbon atoms on [3H]PGE1 uptake was maximal at 6 carbon atoms. Preloading cortical slices with glutarate significantly increased [3H]PGE1 uptake. [3H]PGE1 uptake was inhibited by various eicosanoids and compounds with other structures (p-aminohippurate, benzylpenicillin, estrone-3-sulfate, etc.). These findings suggest that PGE1 uptake by renal cortical slices may be mediated by the members of the organic anion transporter family.
Nagai
Junya
J
Department of Pharmaceutics and Therapeutics, Division of Clinical Pharmaceutical Science, Graduate School of Biomedical Sciences, Hiroshima University, I-2-3 Kasumi, Minami-ku, Hiroshima 734-655 I, Japan.
Nasu
Reishi
R
Murakami
Teruo
T
Takano
Mikihisa
M
D000527:Alprostadil / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D008297:Male; D027361:Organic Anion Transporters / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium
D027361:Organic Anion Transporters; D014316:Tritium; D000527:Alprostadil