title: Changes in exercise-induced gene expression in 5'-AMP-activated protein kinase gamma3-null and gamma3 R225Q transgenic mice.
abstract: 5'-AMP-activated protein kinase (AMPK) is important for metabolic sensing. We used AMPKgamma3 mutant-overexpressing Tg-Prkag3(225Q) and AMPKgamma3-knockout Prkag3-/- mice to determine the role of the AMPKgamma3 isoform in exercise-induced metabolic and gene regulatory responses in skeletal muscle. Mice were studied after 2 h swimming or 2.5 h recovery. Exercise increased basal and insulin-stimulated glucose transport, with similar responses among genotypes. In Tg-Prkag3(225Q) mice, acetyl-CoA carboxylase (ACC) phosphorylation was increased and triglyceride content was reduced after exercise, suggesting that this mutation promotes greater reliance on lipid oxidation. In contrast, ACC phosphorylation and triglyceride content was similar between wild-type and Prkag3-/- mice. Expression of genes involved in lipid and glucose metabolism was altered by genetic modification of AMPKgamma3. Expression of lipoprotein lipase 1, carnitine palmitoyl transferase 1b, and 3-hydroxyacyl-CoA dehydrogenase was increased in Tg-Prkag3(225Q) mice, with opposing effects in Prkag3-/- mice after exercise. GLUT4, hexokinase II (HKII), and glycogen synthase mRNA expression was increased in Tg-Prkag3(225Q) mice after exercise. GLUT4 and HKII mRNA expression was increased in wild-type mice and blunted in Prkag3-/- mice after recovery. In conclusion, the Prkag3(225Q) mutation, rather than presence of a functional AMPKgamma3 isoform, directly promotes metabolic and gene regulatory responses along lipid oxidative pathways in skeletal muscle after endurance exercise.
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, von Eulers väg 4, 4th Floor, S-171 77 Stockholm, Sweden.
authors.forename: Brian R
authors.initials: BR
authors.lastname: Barnes
authors.forename: Yun Chau
authors.initials: YC
authors.lastname: Long
authors.forename: Tatiana L
authors.initials: TL
authors.lastname: Steiler
authors.forename: Ying
authors.initials: Y
authors.lastname: Leng
authors.forename: Dana
authors.initials: D
authors.lastname: Galuska
authors.forename: Jørgen F P
authors.initials: JF
authors.lastname: Wojtaszewski
authors.forename: Leif
authors.initials: L
authors.lastname: Andersson
authors.forename: Juleen R
authors.initials: JR
authors.lastname: Zierath
mesh_terms: D055372:AMP-Activated Protein Kinases; D019943:Amino Acid Substitution; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000201:enzymology / Q000502:physiology; D010805:Physical Conditioning, Animal; D011494:Protein Kinases / Q000172:deficiency* / Q000378:metabolism*; D013550:Swimming; D014280:Triglycerides / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D007328:Insulin; D014280:Triglycerides; D011494:Protein Kinases; C526225:PRKAG3 protein, human; C526221:Prkag3 protein, mouse; D055372:AMP-Activated Protein Kinases; D005947:Glucose