title: Structure and chromosomal DNA binding of the SWIRM domain.
abstract: The evolutionarily conserved Swi3p, Rsc8p and Moira (SWIRM) domain is found in many chromosomal proteins involved in chromatin modifications or remodeling. Here we report the three-dimensional solution structure of the SWIRM domain from the human transcriptional adaptor ADA2alpha. The structure reveals a five-helix bundle consisting of two helix-turn-helix motifs connected by a central long helix, reminiscent of the histone fold. Using structural and biochemical analyses, we showed that the SWIRM domains of human ADA2alpha and SMARC2 bind to double-stranded and nucleosomal DNA, and we identified amino acid residues required for this function. We demonstrated that the ADA2alpha SWIRM domain is colocalized with lysine-acetylated histone H3 in the cell nucleus and that it potentiates the ACF remodeling activity by enhancing accessibility of nucleosomal linker DNA bound to histone H1. These data suggest a functional role of the SWIRM domain in chromatin remodeling.
authors.affiliation: Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York University, One Gustave L. Levy Place, New York, New York 10029, USA.
authors.forename: Chengmin
authors.initials: C
authors.lastname: Qian
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: SiDe
authors.initials: S
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Zeng
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Walsh
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Zhou
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000737:chemistry; D002843:Chromatin / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D002875:Chromosomes / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins; D019077:Helix-Turn-Helix Motifs; D006657:Histones / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000378:metabolism; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002843:Chromatin; D004268:DNA-Binding Proteins; D006657:Histones; D009707:Nucleosomes; C097695:TADA2A protein, human; D014157:Transcription Factors; D004247:DNA