title: Ubiquitin-dependent degradation of adenovirus E1A protein is inhibited by BS69.
abstract: Adenovirus E1A protein perturbs the cell cycle and promotes cell transformation. Although E1A is relatively unstable, regulation of E1A stability has not been fully elucidated. Here, we showed that E1A was ubiquitinated and degraded using a proteasome in vivo system. Interestingly, we found that BS69, one of the E1A-binding proteins, inhibited ubiquitination of E1A. BS69 mutants lacking the MYND domain could not bind to E1A and did not inhibit ubiquitination of E1A. Moreover, we demonstrated that overexpression of BS69 stabilized E1A in vivo. These results suggest that BS69 controls E1A stability via inhibition of ubiquitination.
authors.affiliation: Department of Biochemistry 1, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, Shizuoka 431-3192, Japan.
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Isobe
authors.forename: Chiharu
authors.initials: C
authors.lastname: Uchida
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hattori
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kitagawa
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Oda
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kitagawa
mesh_terms: D017500:Adenovirus E1A Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002460:Cell Line; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D006801:Humans; D009154:Mutation; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D017500:Adenovirus E1A Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D025801:Ubiquitin; C094763:ZMYND11 protein, human; D046988:Proteasome Endopeptidase Complex