Automated colorimetric method for the determination of serum gamma-glutamyl transpeptidase utilizing the Technicon SMAC alkaline phosphatase flow channel.
An automated procedure for the determination of serum gamma-glutamyltranspeptidase using Technicon SMAC alkaline phosphatase flow channel has been developed. The flow variants are insignificant. We use L-gamma-glutamyl-3-carboxy-4-nitroanilide as a very soluble substrate. With the dyalisis procedure, hemoglobin and bilirubin do not interfere. By employing dialysis, the need of blank corrections is therefore eliminated. The procedure can been run at 130 determinations per hour with insignificant carryover and satisfactory precision.
Lippi
U
U
Department of Pathology, P. Cosma Hospital, Camposampiero, Italy.
Cagnin
V
V
D015055:1-Carboxyglutamic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001322:Autoanalysis / Q000379:methods; D003124:Colorimetry / Q000295:instrumentation*; D006168:Guinea Pigs; D006801:Humans; D012016:Reference Values; D005723:gamma-Glutamyltransferase / Q000097:blood*
D015055:1-Carboxyglutamic Acid; C013540:gamma-glutamyl-3-carboxy-4-nitroanilide; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase