[Activating protein-1 members in response to changes of wall-shear stress in osteoblastic cells].
OBJECTIVE
To observe activating protein-1 (AP- 1) members in response to changes of wall-shear stress in osteoblastic cells in vitro.


METHODS
Isolated and purified osteoblastic cells from the calvaria of newborn SD rats were cultured and subcultured. The third generation cells were subjected to wall-shear stress of 0.8 Pa, 1.2 Pa, 1.4 Pa and 1.6 Pa separately. Gene expression of the seven AP-1 members were studied before (0 h) and 10 min, 15 min, 30 min, 60 min after treated with wall-shear stress.


RESULTS
The expression of FosB, c-Fos, c-Jun, JunD and JunB mRNA increased transiently after application of 1.2 Pa wall-shear stress in osteoblastic cells compared to 0.8 Pa , 1.4 Pa and 1.6 Pa stress, and peaked at 15 min.


CONCLUSIONS
Mechanical environment changes in osteoblastic cells induced a dramatic induction of most of the AP-1 members.
Zhu
Fu-dong
FD
Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital, School of Stomatology, Zhejiang University, Hangzhou 310006, China.
Zhao
Shi-fang
SF
D000818:Animals; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D018808:Transcription Factor AP-1
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D018808:Transcription Factor AP-1