Optimizing cell preparation technique to enhance adsorption capacity of pseudomonas putida 5-x to heavy metal ions.
A heavy metal accumulation bacterium Pseudomonas putida 5-x isolated from electroplating effluent was used as biosorbent for heavy metal removal from wastewater. In order to compete with physical adsorbent, the optimization of the cell preparation technique for further improving its heavy metal adsorption capacity was conducted. Experimental results showed that the variation of medium compositions and cell growth age, and different cell pre-treating technique would result in the variation of the cell surface components and structural, hence the variation of adsorption capacity of the cell to heavy metal ions. With optimum cell culture conditions and pretreatment technique, the Cu2+ adsorption capacity of the cell was obviously increased from 51.2 mg g-1 to 89.6 mg g-1. The optimised P. putida 5-x cell could be reused at least five cycles to remove heavy metal ions from electroplating effluent with about 95% removal efficiency.
Wang
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L
School of Environmental Science and Engineering, State Key Laboratory for Pollutant Control and Resource Reuse, Tongji University, Shanghai, China. celwang@yahoo.com
Zhou
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Q
Zheng
G H
GH
D000327:Adsorption; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D001431:Bacteriological Techniques; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D003300:Copper / Q000737:chemistry / Q000302:isolation & purification*; D003470:Culture Media; D004595:Electroplating; D006851:Hydrochloric Acid; D007220:Industrial Waste; D008563:Membrane Lipids / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D009532:Nickel / Q000737:chemistry / Q000302:isolation & purification; D010758:Phosphorus / Q000032:analysis; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D007220:Industrial Waste; D008563:Membrane Lipids; D014874:Water Pollutants, Chemical; D010758:Phosphorus; D003300:Copper; D009532:Nickel; D006851:Hydrochloric Acid