title: Parasite density and impaired biochemical/hematological status are associated with severe clinical aspects of canine visceral leishmaniasis.
abstract: We have performed a detailed investigation in 40 dogs naturally infected with Leishmania infantum (syn. chagasi), subdivided into three groups: asymptomatic (AD = 12), oligosymptomatic (OD = 12) and symptomatic (SD = 16), based on their clinical features. Twenty non-infected dogs (CD) were included as control group. Serological analysis, performed by IFAT and ELISA, demonstrated higher antibodies titers in SD in comparison to the AD. A positive correlation was found between parasite density in the spleen and skin smears as well as the bone marrow parasitism with clinical status of the infection. We observed that the progression of the disease from asymptomatic to symptomatic clinical form was accompanied by intense parasitism in the bone marrow. It is likely that this led to the impaired biochemical/hematological status observed. Finally, we believe that the follow-up of these parameters could be a relevant approach to be used as markers during therapeutic and vaccine evaluations.
authors.affiliation: Laboratório de Parasitologia e Histopatologia, Núcleo de Pesquisas em Ciências Biológicas/NUPEB, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Rua Costa Sena, Minas Gerais, Brazil. alexreis@cpqrr.fiocruz.br
authors.forename: Alexandre B
authors.initials: AB
authors.lastname: Reis
authors.forename: Olindo A
authors.initials: OA
authors.lastname: Martins-Filho
authors.forename: Andréa
authors.initials: A
authors.lastname: Teixeira-Carvalho
authors.forename: Maria G
authors.initials: MG
authors.lastname: Carvalho
authors.forename: Wilson
authors.initials: W
authors.lastname: Mayrink
authors.forename: João C
authors.initials: JC
authors.lastname: França-Silva
authors.forename: Rodolfo C
authors.initials: RC
authors.lastname: Giunchetti
authors.forename: Odair
authors.initials: O
authors.lastname: Genaro
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Corrêa-Oliveira
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D001853:Bone Marrow / Q000469:parasitology; D016022:Case-Control Studies; D018450:Disease Progression; D004283:Dog Diseases / Q000097:blood / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000378:metabolism* / Q000469:parasitology / Q000662:veterinary*; D008297:Male; D011156:Population Density; D012720:Severity of Illness Index
chemical_list: D000913:Antibodies, Protozoan