title: Cloning of cDNA encoding canine endothelin receptors and their expressions in normal tissues.
abstract: The receptors for endothelin (ET) family, ETA and ETB, were molecularly cloned and the expression of ETA and ETB as well as preproendothelin-1 (PPET-1, precursor of ET-1) was examined in normal canine tissues by RT-PCR. The entire open reading frames of the canine ETA and ETB were shown to encode 427 and 442 amino acid residues, respectively, showing from 87.4 to 97.3% sequence similarity to human, mouse, and rat counterparts. ETA and ETB mRNAs were ubiquitously expressed in a variety of canine tissues in this study and PPET-1 mRNA was detected in the tissues except for heart and liver. It was speculated that ET could play an important role in physiological events in most of the organs.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Kanagawa, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Yasuda
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Tsukui
authors.forename: Kenichi
authors.initials: K
authors.lastname: Masuda
authors.forename: Shinpei
authors.initials: S
authors.lastname: Kawarai
authors.forename: Keitaro
authors.initials: K
authors.lastname: Ohmori
authors.forename: Sadatoshi
authors.initials: S
authors.lastname: Maeda
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017931:DNA Primers; D004285:Dogs / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D017466:Receptors, Endothelin / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D017931:DNA Primers; D017466:Receptors, Endothelin