Toward the clinical application of time-domain fluorescence lifetime imaging.
High-speed (video-rate) fluorescence lifetime imaging (FLIM) through a flexible endoscope is reported based on gated optical image intensifier technology. The optimization and potential application of FLIM to tissue autofluorescence for clinical applications are discussed.
Munro
I
I
Imperial College London, Physics Department, Photonics Group, South Kensington Campus, London SW7 2AZ. i.munro@imperial.ac.uk
McGinty
J
J
Galletly
N
N
Requejo-Isidro
J
J
Lanigan
P M P
PM
Elson
D S
DS
Dunsby
C
C
Neil
M A A
MA
Lever
M J
MJ
Stamp
G W H
GW
French
P M W
PM
D000818:Animals; D003199:Computer Systems; D019723:Endoscopes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity