[Integration and expression of beta-endoglucanase I from Trichoderma reesei in brewing yeast].
An integration plasmid pA15-PET for expression and secretion of beta-Endoglucanase I (EG I ) in yeast was constructed by insertion of EG I cDNA between yeast alcohol dehydrogenase promoter and terminator region. The plasmid contained part of yeast rDNA sequence, which was used as a homologous fragment for integration. The EG I cDNA was introduced into an engineered brewing yeast BE9711 containing alpha-acetolactate decarboxylase (alpha-ALDC) encoding gene and integrated onto its rDNA sequence of chromosomal DNA by co-transformation of pA15-PET and a YEP type plasmid pA15TXR carrying G418 resistance. The stable engineered brewing yeast expressing intracellulase alpha-ALDC and extracellular EG I simultaneously were obtained.
Tang
Xiaoying
X
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Qin
Junchuan
J
Tang
Guomin
G
Wang
Aoquan
A
D000426:Alcohol Dehydrogenase / Q000235:genetics; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D044602:Cellulases / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005818:Genetic Engineering; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology*
D005656:Fungal Proteins; D000426:Alcohol Dehydrogenase; D044602:Cellulases; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase