title: ATF5 increases cisplatin-induced apoptosis through up-regulation of cyclin D3 transcription in HeLa cells.
abstract: ATF5 transcription factor plays an essential role in hematopoietic and glioma cell survival and neuronal cell differentiation. Here, we report for the first time the pro-apoptosis role of ATF5 and identify Cyclin D3 as an ATF5-targeted apoptosis-related gene. The ectopic expression of ATF5 in HeLa cells could markedly increase cisplatin-induced apoptosis and the cleavage of Caspase-3, and induce Cyclin D3 mRNA expression via cooperation with E2F1 transcription factor. Moreover, the interference of Cyclin D3 expression by transfection with Cyclin D3 RNAi could protect cells from ATF5-mediated apoptosis induced by cisplatin, indicating the contribution of Cyclin D3 in ATF5-mediated apoptosis. Taken together, these results suggest that ATF5 increases cisplatin-induced apoptosis through up-regulation of Cyclin D3 transcription, which elicits survival signals in HeLa cells.
authors.affiliation: State Key Laboratory of Genetic Engineering and Gene Research Center, Shanghai Medical College of Fudan University, Shanghai 200032, People's Republic of China.
authors.forename: Yuanyan
authors.initials: Y
authors.lastname: Wei
authors.forename: Jianhai
authors.initials: J
authors.lastname: Jiang
authors.forename: Maoyun
authors.initials: M
authors.lastname: Sun
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Chen
authors.forename: Hanzhou
authors.initials: H
authors.lastname: Wang
authors.forename: Jianxin
authors.initials: J
authors.lastname: Gu
mesh_terms: D051696:Activating Transcription Factors / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D056743:Cyclin D3; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D034622:RNA Interference; D014158:Transcription, Genetic; D014162:Transfection; D015854:Up-Regulation
chemical_list: C507532:ATF5 protein, human; D051696:Activating Transcription Factors; C534696:CCND3 protein, human; D056743:Cyclin D3; D016213:Cyclins; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D002945:Cisplatin