Proteome analysis of human amnion and amniotic fluid by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Proteome analysis by 2-DE and PMF by MALDI-TOF MS was performed on human amnion and amniotic fluid at term. Ninety-two soluble and nineteen membrane proteins were identified from amnion. Thirty-five proteins were identified from amniotic fluid. Calgranulin A and B were found in all patients infected with Ureaplasma urealyticum, but not in any of the patients without infection, indicating that they are potential markers of intrauterine infection. Identity of calgranulin A and B was confirmed by MALDI-TOF/TOF MS. This study represents the first extensive analysis of the human amnion and amniotic fluid proteome at term and demonstrates that 2-DE and MALDI-TOF MS is a useful tool for identifying clinically significant biomarkers of problematic pregnancies.
Park
Soo-Jin
SJ
Department of Chemistry, Seoul National University, Gwanak-gu, Seoul, Korea 151-747.
Yoon
Won-Gap
WG
Song
Jin-Su
JS
Jung
Hyun Sook
HS
Kim
Chong Jai
CJ
Oh
Soo Young
SY
Yoon
Bo Hyun
BH
Jung
Guhung
G
Kim
Hie-Joon
HJ
Nirasawa
Takashi
T
D000595:Amino Acid Sequence; D000650:Amnion / Q000737:chemistry*; D000653:Amniotic Fluid / Q000737:chemistry*; D001483:Base Sequence; D040501:Calgranulin A / Q000302:isolation & purification; D040502:Calgranulin B / Q000737:chemistry / Q000302:isolation & purification; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D016869:Ureaplasma Infections / Q000378:metabolism
D040501:Calgranulin A; D040502:Calgranulin B; D017931:DNA Primers; D020543:Proteome