Enzyme-electropolymer-based amperometric biosensors: an innovative platform for time-temperature integrators.
A novel exogenous time-temperature integrator (TTI) based on an amperometric glucose oxidase biosensor is presented. The TTI consists of the enzyme entrapped within an electrochemically generated poly(o-phenylenediamine) (PoPD) thin film deposited on the interior wall of a platinum (Pt) or a platinized stainless steel (Pt-SS) capsule. After thermal treatment, the TTI is mounted in a continuous flow system and connected to a potentiostat for amperometric detection of residual enzyme activity. A measurement is completed within 10 min. Isothermal treatments were carried out between 70 and 79.7 degrees C. Thermal inactivation of the immobilized enzyme followed apparent first-order kinetics with z values of 6.2 +/- 0.6 and 6.6 +/- 0.8 degrees C for Pt and Pt-SS capsules, respectively. These z values suggest that the proposed TTIs have the potential to assess pasteurization processes that target microorganism such as Listeria monocytogenes and Escherichia coli O157:H7.
Reyes-De-Corcuera
José I
JI
Department of Food Science and Human Nutrition, Washington State University, Pullman, Washington 99164-6376, USA.
Cavalieri
Ralph P
RP
Powers
Joseph R
JR
Tang
Juming
J
Kang
Dong H
DH
D015374:Biosensing Techniques / Q000295:instrumentation*; D005516:Food Microbiology; D005949:Glucose Oxidase; D011108:Polymers; D011199:Potentiometry / Q000295:instrumentation; D013696:Temperature; D013995:Time
D011108:Polymers; D005949:Glucose Oxidase