Antichagasic activity of komaroviquinone is due to generation of reactive oxygen species catalyzed by Trypanosoma cruzi old yellow enzyme.
A novel potent trypanocidal diterpene, komaroviquinone, was reduced by Trypanosoma cruzi old yellow enzyme (TcOYE) to its semiquinone radical. The reductase activity in trypanosome lysates was completely immunoabsorbed by anti-TcOYE antibody. Since TcOYE is expressed throughout the T. cruzi life cycle, komaroviquinone is an interesting candidate for developing new antichagasic drugs.
Uchiyama
Nahoko
N
Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
Kabututu
Zakayi
Z
Kubata
Bruno K
BK
Kiuchi
Fumiyuki
F
Ito
Michiho
M
Nakajima-Shimada
Junko
J
Aoki
Takashi
T
Ohkubo
Kei
K
Fukuzumi
Shunichi
S
Martin
Samuel K
SK
Honda
Gisho
G
Urade
Yoshihiro
Y
D000818:Animals; D002384:Catalysis; D014355:Chagas Disease / Q000188:drug therapy*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D011809:Quinones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014344:Trypanocidal Agents / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use*; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000235:genetics
D004224:Diterpenes; D011809:Quinones; D017382:Reactive Oxygen Species; D014344:Trypanocidal Agents; C508550:komaroviquinone; D009252:NADPH Dehydrogenase