title: Oxidative stress by monoamine oxidase mediates receptor-independent cardiomyocyte apoptosis by serotonin and postischemic myocardial injury.
abstract: BACKGROUND
  Serotonin (5-hydroxytryptamine [5-HT]), released by activated platelets during cardiac ischemia, is metabolized by the mitochondrial enzyme monoamine oxidase A (MAO-A). Because hydrogen peroxide is one of the byproducts of 5-HT degradation by MAO-A, we investigated the potential role of reactive oxygen species generated by MAOs in 5-HT-dependent cardiomyocyte death and post-ischemia-reperfusion cardiac damage.
  
  
  RESULTS
  Treatment of isolated adult rat cardiomyocytes with 5-HT induced intracellular oxidative stress and cell apoptosis. The apoptotic cascade triggered by 5-HT involves release of cytochrome c, upregulation of proapoptotic Bax protein, and downregulation of antiapoptotic Bcl-2 protein. These effects were prevented by inhibition of amine transporter or MAO, antioxidants, or iron chelation. In contrast, cardiomyocyte apoptosis was only slightly affected by the 5-HT(2B) receptor antagonist SB 206553. In vivo, inhibition of MAO-A largely reduced myocardial ultrastructural damage induced by 30 minutes of ischemia followed by 60 minutes of reperfusion in the rat heart. Cardioprotective effects of MAO inhibitors were associated with the prevention of postischemic oxidative stress, neutrophil accumulation, and mitochondrial-dependent cell death and were not reverted by SB 206553. Administration of MAO-A inhibitors during ischemia was still effective in preventing cardiac damage.
  
  
  CONCLUSIONS
  Our results supply the first direct evidence that oxidative stress induced by MAO is responsible for receptor-independent apoptotic effects of 5-HT in cardiomyocytes and postischemic myocardial injury. These findings provide new insight into the mechanisms of 5-HT action in the heart and may constitute the basis for novel therapies.
authors.affiliation: INSERM U388, 31432 Toulouse Cedex 4, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Bianchi
authors.forename: Oxana
authors.initials: O
authors.lastname: Kunduzova
authors.forename: Emanuela
authors.initials: E
authors.lastname: Masini
authors.forename: Claudie
authors.initials: C
authors.lastname: Cambon
authors.forename: Daniele
authors.initials: D
authors.lastname: Bani
authors.forename: Laura
authors.initials: L
authors.lastname: Raimondi
authors.forename: Marie-Helene
authors.initials: MH
authors.lastname: Seguelas
authors.forename: Silvia
authors.initials: S
authors.lastname: Nistri
authors.forename: Wilson
authors.initials: W
authors.lastname: Colucci
authors.forename: Nathalie
authors.initials: N
authors.lastname: Leducq
authors.forename: Angelo
authors.initials: A
authors.lastname: Parini
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D006352:Heart Ventricles / Q000166:cytology; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010586:Phagocytes / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D008996:Monoamine Oxidase Inhibitors; D051028:bcl-2-Associated X Protein; D012701:Serotonin; D006861:Hydrogen Peroxide; D008995:Monoamine Oxidase; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases