Isolation of mutagenic beta-carboline derivatives after nitrite treatment of maillard reaction mixtures and analysis of these compounds from foodstuffs and human urine.
Mixtures of carbohydrate decomposition products and L-tryptophan were incubated at pH 7.0 and 37 degrees C for 4 weeks. These mixtures exhibited mutagenic activity toward S. typhimurium TA 100 without metabolic activation after a nitrite treatment at pH 4.0. Four beta-carboline derivatives were isolated as premutagens from mixtures of methylglyoxal and furfural. These premutagens were also found to be contained in daily foodstuffs and human urine samples.
Masuda
Shuichi
S
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences and COE Program in the 21st Century, University of Shizuoka, Japan. masudas@u-shizuoka-ken.ac.jp
Kanamori
Hisayuki
H
Kinae
Naohide
N
D002241:Carbohydrates; D002243:Carbolines / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology / Q000652:urine; D005504:Food Analysis; D006801:Humans; D015416:Maillard Reaction; D009153:Mutagens / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D009573:Nitrites; D014364:Tryptophan; D014556:Urine / Q000737:chemistry
D002241:Carbohydrates; D002243:Carbolines; D009153:Mutagens; D009573:Nitrites; D014364:Tryptophan