The effects of paradoxical sleep deprivation on estrous cycles of the female rats.
The present purpose was to examine how sleep deprivation affects the estrous cycle of the female rat. Paradoxical sleep-deprived (PSD) adult female Wistar rats were compared to home-cage control (CTRL) on their estrous cyclicity. Forty-four PSD and forty-four CTRL female rats were distributed into 4 subgroups of 11 animals each according to the phase of estrous cycle and were subjected to sleep deprivation for 96 h by the multiple platform technique. After PSD period, vaginal estrous cycles were taken for an additional 9 days. Animals that were submitted to PSD in diestrus phase (PSD-diestrus) had their estrous cycles disrupted during the recovery period by showing a constant diestrus during the first week. As for hormone alterations, progesterone concentrations were statistically higher in PSD-diestrus compared to respective phase control and to PSD-proestrus and PSD-estrus rats while CTRL-metestrus had higher levels than CTRL-proestrus and estrus groups. Testosterone was significantly decreased in PSD-estrus in relation to PSD-proestrus and PSD-diestrus groups and was lower in CTRL-diestrus rats than in home-cage rats in proestrus. In addition, PSD-diestrus phase exhibited higher concentrations of corticosterone and lower estrogen than the respective control rats. These data indicate that PSD may modulate the ovarian hormone release through alterations in hormonal-neurochemical mechanisms.
Antunes
Isabela B
IB
Department of Psychobiology, Universidade Federal de São Paulo, Escola Paulista de Medicina, UNIFESP/EPM, Rua Napoleão de Barros, 925, Vila Clementino 04024-002, São Paulo, SP, Brazil.
Andersen
Monica L
ML
Baracat
Edmundo C
EC
Tufik
Sergio
S
D000818:Animals; D003345:Corticosterone / Q000097:blood; D004958:Estradiol / Q000097:blood; D004970:Estrone / Q000097:blood; D030762:Estrous Cycle / Q000097:blood*; D005260:Female; D011374:Progesterone / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D012892:Sleep Deprivation / Q000097:blood*; D012895:Sleep, REM / Q000502:physiology*; D013739:Testosterone / Q000097:blood*
D004970:Estrone; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D003345:Corticosterone