title: Spiroplasma leucomae sp. nov., isolated in Poland from white satin moth (Leucoma salicis L.) larvae.
abstract: Spiroplasma sp. strain SMA(T), isolated in Poland from white satin moth larvae, Leucoma salicis L. (Lepidoptera: Lymantriidae), was serologically distinct from other Spiroplasma species, groups or subgroups. Dark-field microscopy of the cells revealed the classical helical shape and subsequent transmission electron microscopy revealed cells surrounded by only a single cell membrane (lacking a cell wall). Growth of strain SMA(T) occurred in M1D medium at 30 degrees C. Strain SMA(T) catabolized both glucose and arginine, but did not hydrolyse urea. The G+C content of the DNA was 24+/-1 mol% as determined by melting temperature analysis. Serological analysis revealed a very weak cross-reactivity (positive reaction only up to a 1 : 80 dilution) with two Spiroplasma strains, 277F (Spiroplasma sp. group I-4) and LB-12 (Spiroplasma sp. group I-5). Strain SMA(T) (=ATCC BAA-521T=NBRC 100392T) is designated the type strain of a novel species, Spiroplasma leucomae sp. nov. (class Mollicutes: order Entomoplasmatales: family Spiroplasmataceae).
authors.affiliation: Department of Biological Sciences, Towson University, Towson, MD 21252, USA.
authors.forename: Marietta A
authors.initials: MA
authors.lastname: Oduori
authors.affiliation: Department of Biocontrol and Quarantine, Institute of Plant Protection, Poznan, Poland.
authors.forename: Jerzy J
authors.initials: JJ
authors.lastname: Lipa
authors.affiliation: Department of Biological Sciences, Towson University, Towson, MD 21252, USA.
authors.forename: Gail E
authors.initials: GE
authors.lastname: Gasparich
mesh_terms: D000818:Animals; D001482:Base Composition; D003470:Culture Media; D008969:Molecular Sequence Data; D009036:Moths / Q000382:microbiology*; D011044:Poland; D013149:Spiroplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003470:Culture Media