Role of the kinesin-2 family protein, KIF3, during mitosis.
During mitosis, kinesin and dynein motor proteins play critical roles in the equal segregation of chromosomes between two daughter cells. Kinesin-2 is composed of two microtubule-based motor subunits, KIF3A/3B, and a kinesin-associated protein known as KAP3, which links KIF3A/3B to cargo that is carried to cellular organelles along microtubules in interphase cells. We have shown here that the kinesin-2 complex is localized with components of the mitotic apparatus such as spindle microtubules and centrosomes. Furthermore, we found that expression of a mutant KIF3B, which is able to associate with KIF3A but not KAP3 in NIH3T3 cells, caused chromosomal aneuploidy and abnormal spindle formation. Our data suggested that the kinesin-2 complex plays an important role not only in interphase but also in mitosis.
Haraguchi
Keiko
K
Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Japan.
Hayashi
Tomoatsu
T
Jimbo
Takeshi
T
Yamamoto
Tadashi
T
Akiyama
Tetsu
T
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D007399:Interphase; D016547:Kinesins / Q000737:chemistry / Q000502:physiology*; D051379:Mice; D008938:Mitosis; D041681:NIH 3T3 Cells; D010766:Phosphorylation
D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C087415:KIF3A protein, human; C095899:KIF3B protein, human; C484407:KIFAP3 protein, human; C490202:Kif3a protein, mouse; C490672:Kif3b protein, mouse; C100815:Kifap3 protein, mouse; D016547:Kinesins