title: Purification and characterization of aldoxime dehydratase of the head blight fungus, Fusarium graminearum.
abstract: Fungal aldoxime dehydratase (Oxd) of Fusarium graminearum MAFF305135 was purified and characterized for the first time from its overexpressing Escherichia coli transformant. The enzyme showed about 20% identity with known Oxds, and had similar enzymatic properties with nitrilase-linked Oxd from the Bacillus strain. It belongs to a group of phenylacetaldoxime dehydratases (EC 4.99.1.7), based on its substrate specificity and kinetic analysis.
authors.affiliation: Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kato
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Asano
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D005670:Fusarium / Q000201:enzymology*; D006836:Hydro-Lyases / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008190:Lyases; D017385:Sequence Homology; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D008190:Lyases; D006836:Hydro-Lyases; C412124:aldoxime dehydratase; C404242:oxd protein, Bacillus