[Detection of anti-Trichinella spiralis antibody by indirect ELISA using recombinant protein as antigen].
OBJECTIVE
To study the specificity, sensitivity of diagnostic antigen for Trichinella spiralis (T.s).


METHODS
T668 recombinant protein from highly efficient expression of newborn larvae stage-specific gene of T.s in E. coli as diagnostic antigen. By using negative and positive sera from rabbit, pig, human as the first antibody, and goat-anti-rabbit IgG, goat-anti-pig IgG, goat-anti-human IgG labeled with HRP as the secondary antibody, indirect ELISA method was established for detecting anti-T.s antibody, while excretory-secretory (ES) antigen of T.s muscle larvae was used as control.


RESULTS
Sera from rabbit, pig and human were detected by T668 recombinant protein as antigen, which showed a positive rate of 100% with 0.016 microg/well. There was no difference on the results between the T668 recombinant antigen and the ES antigen for diagnosing T.s-infection.


CONCLUSIONS
The T668 recombinant antigen is promising in substituting ES antigen in the detection of anti-T.s antibody.
Niu
Ting-Xian
TX
Lanzhou General Hospital of PLA, Lanzhou 730050, China.
Pan
Fei-Fei
FF
Liu
Ming-Yuan
MY
Lu
Qiang
Q
Fu
Bao-Quan
BQ
Pascal
Boireau
B
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D015801:Helminth Proteins; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D007814:Larva / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity; D013552:Swine; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis* / Q000276:immunology
D000909:Antibodies, Helminth; D000953:Antigens, Protozoan; D015801:Helminth Proteins; D007106:Immune Sera; D007074:Immunoglobulin G; D011994:Recombinant Proteins