St. Louis encephalitis virus: first isolation from a human in São Paulo State, Brazil.
This paper reports the isolation of St. Louis encephalitis virus (SLEV) from a febrile human case suspected to be dengue, in São Pedro, São Paulo State. A MAC-ELISA done on the patient's acute and convalescent sera was inconclusive and hemagglutination inhibition test detected IgG antibody for flaviviruses. An indirect immunofluorescent assay done on the C6/36 cell culture inoculated with the acute serum was positive for flaviviruses but negative when tested with dengue monoclonal antibodies. RNA extracted from the infected cell culture supernatant was amplified by RT-PCR in the presence of NS5 universal flavivirus primers and directly sequenced. Results of BLAST search indicated that this sequence shares 93% nucleotide similarity with the sequence of SLEV (strain-MSI.7), confirmed by RT-PCR performed with SLEV specific primers. Since SLEV was identified as the cause of human disease, it is necessary to improve surveillance in order to achieve early detection of this agent in the state of São Paulo and in Brazil. This finding is also an alert to health professionals about the need for more complete clinical and epidemiological investigations of febrile illnesses as in the reported case. SLEV infections can be unrecognized or confused with other ones caused by an arbovirus, such as dengue.
Rocco
Iray M
IM
Instituto Adolfo Lutz, São Paulo, SP, Brazil. imrocco@ial.sp.gov.br
Santos
Cecília L S
CL
Bisordi
Ivani
I
Petrella
Selma M C N
SM
Pereira
Luiz E
LE
Souza
Renato P
RP
Coimbra
Terezinha L M
TL
Bessa
Thirsa A F
TA
Oshiro
Fabiola M
FM
Lima
Luciana B Q
LB
Cerroni
Matheus P
MP
Marti
Antonia T
AT
Barbosa
Vera M
VM
Katz
Gizelda
G
Suzuki
Akemi
A
D000914:Antibodies, Viral / Q000097:blood*; D001938:Brazil; D004665:Encephalitis Virus, St. Louis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004674:Encephalitis, St. Louis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D012367:RNA, Viral