Chemical sympathectomy attenuates myenteric but not dorsal vagal complex Fos-like immunoreactivity induced by cholecystokinin-8 in the rat.
Vagotomy and capsaicin treatment attenuate dorsal vagal complex (DVC) but not myenteric Fos-like immunoreactivity (Fos-LI) induced by cholecystokinin-8 (CCK-8). The goal of this experiment is to test the role of the sympathetic nervous system in the pathway by which CCK-8 increases myenteric Fos-LI. Adult male Sprague-Dawley rats were pretreated with guanethidine sulfate (40 mg/kg daily for 5 weeks) or vehicle intraperitoneally (IP), and injected with CCK-8 (40 microg/kg) or saline IP. Fos-LI was then quantified in the DVC and the myenteric neurons of the duodenum and jejunum using a diaminobenzidine reaction. Guanethidine pretreatment attenuated myenteric but not DVC Fos-LI induced by CCK-8. These findings demonstrate that sympathetic neurons play a role in mediating the myenteric Fos-LI response to CCK. They also suggest differential mediation of myenteric and DVC responses to CCK.
Gulley
Stephen
S
Gastroenterology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL 36088, USA.
Raboin
Shannon J
SJ
Henley
Sheryce C
SC
Chan
Wai-Chu
WC
Sharma
Sanjay K
SK
Billups
Leonard H
LH
Esdaile
Alton R
AR
Sullivan
Cherese N
CN
Moran
Timothy H
TH
Sayegh
Ayman I
AI
D000704:Analysis of Variance; D000818:Animals; D002766:Cholecystokinin / Q000502:physiology*; D006145:Guanethidine / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D009197:Myenteric Plexus / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013563:Sympathectomy, Chemical; D017779:Sympathetic Fibers, Postganglionic / Q000187:drug effects / Q000378:metabolism*; D013565:Sympatholytics / Q000494:pharmacology; D014628:Vagotomy; D014630:Vagus Nerve / Q000187:drug effects / Q000378:metabolism*
D010446:Peptide Fragments; D016760:Proto-Oncogene Proteins c-fos; D013565:Sympatholytics; C410885:cholecystokinin 8; D002766:Cholecystokinin; D006145:Guanethidine