title: Fluorescence microscopy today.
abstract: Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues. The impact of these and other new imaging methods in biophysics, neuroscience, and developmental and cell biology has been remarkable. Further advances in fluorophore design, molecular biological tools and nonlinear and hyper-resolution microscopies are poised to profoundly transform many fields of biological research.
authors.affiliation: Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, New York, New York 10027, USA. rmy5@columbia.edu
authors.forename: Rafael
authors.initials: R
authors.lastname: Yuste
mesh_terms: D000818:Animals; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins