Effect of aminooxyacetic acid on extracellular level of D-serine in rat striatum: an in vivo microdialysis study.
To elucidate the effect of an inhibitor of pyridoxal phosphate-dependent enzymes, aminooxyacetic acid, on the activity of serine racemase in vivo, we have investigated the effect of aminooxyacetic acid on the extracellular concentration of D-serine in the rat striatum using an in vivo microdialysis technique. The intrastriatal perfusion of aminooxyacetic acid caused a significant decline in the extracellular concentration of D-serine. These data, together with the fact that serine racemase is a pyridoxal phosphate-dependent enzyme, suggest that the aminooxyacetic acid-induced reduction of the extracellular D-serine may be at least in part due to the drug's ability to inhibit serine racemase.
Hashimoto
Atsushi
A
Department of Pharmacology, School of Medicine, Tokai University, Isehara, Kanagawa, 259-1143, Japan. hashimot@is.icc.u-tokai.ac.jp
Yoshikawa
Masanobu
M
D000625:Aminooxyacetic Acid / Q000494:pharmacology*; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005110:Extracellular Space / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D019998:Racemases and Epimerases / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar; D012694:Serine / Q000031:analogs & derivatives* / Q000378:metabolism*; D026941:Sodium Channel Blockers / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D000099300:Serine Racemase
D000625:Aminooxyacetic Acid; D004791:Enzyme Inhibitors; D019998:Racemases and Epimerases; D012694:Serine; D026941:Sodium Channel Blockers; D013779:Tetrodotoxin; D000099300:Serine Racemase