title: [Purification and some properties of bacteriolytic enzyme R1 from Streptomyces griseus RX-17].
abstract: Bacteriolytic enzyme R1 was purified to electrophoretic homogeneity with the recovery of 6.89% activity by ammonium sulfate precipitation, CM-Sephadex C - 50, CM-Sepharose Fast Flow and Sephadex G-75 chromatography from the culture supernatant of Streptomyces griseus RX-17. The molecular weight and PI of R1 were 16.8 kD and 9.10. The optimal temperature and pH for R1 against Streptococcus mutans Ingbritt were 70 degrees C and 6.6, respectively. Below 50 degrees C and at range pH 6 - 10, R1 was stable. While treated at 60 degrees C for 1 hour, the residual activity was only about 20.3%. Zn2+, Cu2+, Fe2+, Cd2+ and Pb2+ could completely inactivate the enzyme. Chelating agents, hydroxylamine hydrochloriae, Monoiodoacetic acid inhibited the lytic activity against Streptococcus mutans Ingbritt, whereas Mg2+, 2-Mercaptoethanol and some surfactants could stimulate the activity. The enzyme had a broad bacteriolytic spectrum against many G+, G- bacteria which were resistant to egg-white lysozyme. Especially high activity was shown on Streptococcus mutans, Staphylococcus aureus and Lactobaillus.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Guangwen
authors.initials: G
authors.lastname: Ren
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Tu
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001419:Bacteria / Q000187:drug effects; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D010450:Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D004795:Enzyme Stability; D008970:Molecular Weight; D013305:Streptomyces griseus / Q000737:chemistry / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D010450:Endopeptidases