Comparative evaluation of hexokinase isoenzyme typing and antigen detection for differentiation of Entamoeba histolytica and Entamoeba dispar.
Entamoeba histolytica, the causative organism of invasive amebiasis is a potential pathogen, while asymptomatic infection is caused by E. dispar. Differentiation of the species is not possible on the basis of morphological characters by microscopic examination. In the present study an attempt has been made to differentiate E. histolytica from E. dispar in 45 isolates obtained from culture and direct stool samples respectively on the basis of hexokinase isoenzyme analysis and Tech Lab ELISA. A 100% correlation was found between these two techniques. However, Tech Lab E. histolytica antigen detection test was found to be both rapid and technically simple. Its use in diagnosis and epidemiological studies is recommended.
Bansal
Devendra
D
Department of Parasitology, Post-Graduate Institute of Medical Education & Research, Chandigarh, India.
Sehgal
Rakesh
R
Chawla
Yogesh
Y
Mahajan
Ramesh Chander
RC
Malla
Nancy
N
D000818:Animals; D000953:Antigens, Protozoan; D004747:Entamoeba / Q000145:classification / Q000276:immunology; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000175:diagnosis* / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D006593:Hexokinase / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000145:classification
D000953:Antigens, Protozoan; D007527:Isoenzymes; D006593:Hexokinase