[The role of plasmatic erythrocytic membrane in formation of hypoxia in patients with chronic cardiac failure].
OBJECTIVE
To study oxygen supply to erythrocytes in patients with chronic cardiac failure as a factor provoking hypoxia.


METHODS
The trial enrolled 12 men with chronic cardiac failure (CCF) of functional NYHA class II aged 37 to 74 years (group A). Group B--healthy donors (n = 10) aged 20-40 years. Conformation changes of hemoporphirine were detected with degradation spectroscopy, viscosity of plasmatic membrane--with resonance spectroscopy. Degradation spectroscopy estimated content of Hb-ligant complexes as 0.34 +/- 0.013; complexes I Hb-NO 0.73 +/- 0.11, complexes II Hb-NO 0.28 +/- 0.08 (group A).


RESULTS
Electronic-paramagnetic spectroscopy has estimated: S--0.716 +/- 0.005 (group A), 0.667 +/- 0.011 (group B). In group B- 0.5 +/- 0.08; 0.41 +/- 0.22; 0.55 +/- 0.12, respectively.


CONCLUSIONS
Patients with CHF have high microviscosity of plasmatic erythrocytic membrane near location of polar lipid groups; subnormal content of oxyhemoglobin and elevation of the number of complexes of nitric hemoglobin-oxide; altered binding of nitric oxide with hemoglobin.
Maksimov
G V
GV
Rodnenkov
O V
OV
Luneva
O G
OG
Brazhe
N A
NA
Parshina
E Iu
EIu
Rubin
A B
AB
Chazov
E I
EI
D000328:Adult; D000368:Aged; D002303:Cardiac Output, Low / Q000150:complications*; D002462:Cell Membrane / Q000737:chemistry; D002908:Chronic Disease; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000737:chemistry*; D004912:Erythrocytes / Q000166:cytology; D006415:Hematoporphyrins / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000175:diagnosis / Q000209:etiology*; D008024:Ligands; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000032:analysis; D010101:Oxygen Consumption; D010108:Oxyhemoglobins / Q000032:analysis; D011487:Protein Conformation; D014783:Viscosity
D006415:Hematoporphyrins; D006454:Hemoglobins; D008024:Ligands; D010108:Oxyhemoglobins; D009569:Nitric Oxide