title: Evaluation of the epidemiological importance of classical swine fever infected, E2 sub-unit marker vaccinated animals with RT-nPCR positive blood samples.
abstract: It has been demonstrated that pigs that have been double vaccinated with an E2 sub-unit marker vaccine and that are infected with classical swine fever virus (CSFV) through a natural contact infection may react positive in a CSFV detecting RT-nPCR test, whereas no virus could be isolated by using the conventional virus isolation (VI) technique. To evaluate whether these vaccinated and infected pigs may spread the virus, three experiments were set up. In the first, susceptible pigs were inoculated with serum originating from vaccinated RT-nPCR positive pigs. In the second, vaccinated RT-nPCR positive pigs were brought into contact with sentinel animals. In the third, vertical transmission was evaluated in RT-nPCR positive vaccinated pregnant gilts. In the first two experiments, no proof of virus transmission was found, whereas in the third vertical transmission was observed. The conclusion is that in vaccinated pigs that are positive in RT-nPCR but negative in VI, the level of circulating virus is probably not high enough for horizontal transmission, whereas vertical transmission of the virus is possible.
authors.affiliation: Unit of Veterinary Epidemiology, Faculty of Veterinary Medicine, Department of Reproduction, Obstetrics and Herd Health, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. jeroen.dewulf@ugent.be
authors.forename: J
authors.initials: J
authors.lastname: Dewulf
authors.forename: F
authors.initials: F
authors.lastname: Koenen
authors.forename: S
authors.initials: S
authors.lastname: Ribbens
authors.forename: A
authors.initials: A
authors.lastname: Haegeman
authors.forename: H
authors.initials: H
authors.lastname: Laevens
authors.forename: A
authors.initials: A
authors.lastname: De Kruif
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D006691:Classical Swine Fever / Q000517:prevention & control* / Q000635:transmission*; D006692:Classical Swine Fever Virus / Q000276:immunology* / Q000302:isolation & purification; D018562:Disease Transmission, Infectious / Q000662:veterinary; D005260:Female; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary; D008297:Male; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D014611:Vaccination / Q000662:veterinary*; D022581:Vaccines, Marker; D022223:Vaccines, Subunit; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D022581:Vaccines, Marker; D022223:Vaccines, Subunit; D014765:Viral Vaccines