A colorimetric method for detection and quantification of chlorinating activity of hemeperoxidases.
A method of detecting and assaying the halogenating activity of hemeperoxidases using the colored substrate, thionin, is reported here. In the presence of suitable amounts of peroxide and chloride, chloroperoxidase chlorinates thionin and bleaches the intense color of this substrate. The kinetics was quite similar to that of the established monochlorodimedone assay. Therefore, the thionin assay can be taken as an index of chlorinating activity. This reaction affords an escape from background problems and allows easy processing of large volumes of samples.
Manoj
Kelath Murali
KM
Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. kmm@mail.wsu.edu
Hager
Lowell P
LP
D010544:Peroxidases / Q000032:analysis*; D010640:Phenothiazines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013997:Time Factors
D010640:Phenothiazines; D010544:Peroxidases; C009469:thionine