The comet assay to determine the mode of cell death for the ultrasonic delivery of doxorubicin to human leukemia (HL-60 Cells) from Pluronic P105 micelles.
This notes examines the mode of cell death of HL-60 cells exposed to 70 kHz and 1.3 W/cm(2) ultrasound in the presence of 1% Pluronic P105 and 1.67 microg/ml doxorubicin (Dox). The cells were ultrasonicated for 30, 60, and 120 minutes. They were then lysed, electrophorised, stained using propidium iodide, and their DNA profile captured using a fluorescent microscope. The gradual DNA damage observed and the comet tails captured after one and two hours of insonation suggest that the mode of cell killing is apoptosis.
Husseini
Ghaleb A
GA
Department of Chemical Engineering, American University of Sharjah, United Arab Emirates. ghusseini@ausharjah.edu
O'Neill
Kim L
KL
Pitt
William G
WG
D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects*; D020552:Comet Assay; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D008823:Micelles; D020442:Poloxamer / Q000008:administration & dosage*; D014464:Ultrasonic Therapy
D000903:Antibiotics, Antineoplastic; D004273:DNA, Neoplasm; D008823:Micelles; D020442:Poloxamer; D004317:Doxorubicin