title: Effects of 0.4 T rotating magnetic field exposure on density, strength, calcium and metabolism of rat thigh bones.
abstract: The current study investigated the effects of 0.4 T rotary non-uniform magnetic field (RMF) exposure on bone density in ovariectomized (OVX) rats. Results showed that many bone indexes are significantly elevated after RMF exposure compared to the control OVX group and confirmed mechanistic evidence that strong magnetic field (MF) exposure could effectively increase bone density and might be used to treat osteoporosis. Synergy of daily RMF exposure (30 min a day for 30 days using an 8 Hz rotary 0.4 T MF) with calcium supplement tended to increase the indexes of thigh bone density, energy absorption, maximum load, maximum flexibility, and elastic deformation as compared to those of untreated OVX control group. Results also revealed that the indexes of alkaline phosphatase (ALP), serum phosphate, and serum calcium were higher in rats exposed to RMF with calcium than in the untreated OVX control group. Changes in bone mineral density (BMD) and bone mineral content (BMC) were observed in rats for three months including the first month RMF exposure. Bone density in rats exposed each day for 60 min increased during 1-month exposure and continued to increase during the post-exposure period. Furthermore, bone density and calcium content in rats exposed for 90 min daily decreased initially in the exposure month; however, ratio of increase was well above the control values by the end of the post-exposure period suggesting possible window and delayed effects. The study indicated that RMF exposure to both male and OVX female rats for 120 min a day over 15 day period should effectively promote increase of bone calcium contents (BCC) and bone-specific alkaline phosphatase (BAP) in rats thigh bone as well as a corresponding decrease in deoxypyridinoline crosslinks (DPD).
authors.affiliation: Department of Biotechnology, Shenzhen University, China. zxy@szu.edu.cn
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015519:Bone Density; D002118:Calcium / Q000378:metabolism*; D004958:Estradiol / Q000097:blood; D005260:Female; D005269:Femur / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II; D008280:Magnetics; D008297:Male; D010024:Osteoporosis / Q000628:therapy; D010052:Ovariectomy; D011506:Proteins / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012399:Rotation; D013718:Tensile Strength
chemical_list: D000596:Amino Acids; C035170:IGF2 protein, human; D011506:Proteins; D004958:Estradiol; D007335:Insulin-Like Growth Factor II; C036020:deoxypyridinoline; D000469:Alkaline Phosphatase; D002118:Calcium