title: Fluorescence in situ hybridization reanalysis of day-6 human blastocysts diagnosed with aneuploidy on day 3.
abstract: OBJECTIVE
  To determine the concordance of day-6 blastocyst analysis with the day-3 fluorescence in situ hybridization (FISH) aneuploidy diagnosis.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  In vitro fertilization laboratory.
  
  
  METHODS
  Six hundred sixty embryos were included from 94 IVF/intracytoplasmic sperm injection patients undergoing preimplantation genetic diagnosis.
  
  
  METHODS
  None.
  
  
  METHODS
  Single blastomeres biopsied on day 3. Aneuploidy screening for chromosomes 13, 18, 21, X, and Y were analyzed. Left-over blastocysts were reanalyzed on day 6.
  
  
  RESULTS
  Among the 660 embryos evaluated, 367 (55.6%) were euploid and 281 (42.6%) were aneuploid. Of the euploid embryos, 213 embryos were transferred, 68 were frozen on day 5, and 86 were left. All 281 aneuploid embryos were further cultured, and 55 (19.6%) progressed to blastocysts. When FISH reanalysis was performed, 33 of 55 blastocysts (60%) were confirmed aneuploid in concordance with the day-3 diagnosis. However, 22 of 55 blastocysts (40%) were determined to be euploid. In addition, 207 aneuploid embryos (73.7%) arrested before day 6, as opposed to 32 of the 86 euploid embryos (37.2%).
  
  
  CONCLUSIONS
  Day-3 single-cell embryo biopsy reveals that aneuploidy can be confirmed in 60.7% of the blastocysts on reanalysis. The majority of discordance is most likely due to embryo mosaicism and possibly a limited ability to "self-correct."
authors.affiliation: ART Reproductive Center, Beverly Hills, California 90210, USA. mli@artreproductivecenter.com
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.forename: Catherine Marin
authors.initials: CM
authors.lastname: DeUgarte
authors.forename: Mark
authors.initials: M
authors.lastname: Surrey
authors.forename: Hal
authors.initials: H
authors.lastname: Danzer
authors.forename: Alan
authors.initials: A
authors.lastname: DeCherney
authors.forename: David L
authors.initials: DL
authors.lastname: Hill
mesh_terms: D000782:Aneuploidy; D001755:Blastocyst / Q000502:physiology*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods* / Q000592:standards*; D019836:Preimplantation Diagnosis / Q000379:methods* / Q000592:standards*; D015203:Reproducibility of Results; D012189:Retrospective Studies