Characterisation of a new splice variant of MASK-BP3(ARF) and MASK human genes, and their expression patterns during haematopoietic cell differentiation.
In this study we report the characterisation of a new splice variant, here denominated splice variant 4 (accession number AF258557) of the human Multiple Ankyrin repeats Single KH domain (hMASK) (accession number AF521882) and the hMASK-4E-Binding Protein 3 Alternative Reading Frame (hMASK-BP3(ARF)) (accession number AF521883), containing a number of ANK-repeat motifs. Ankyrin (ANK) repeat-containing proteins carry out a wide variety of biological activities and are involved in processes, such as cell differentiation and transcriptional regulation. The present study reports the computer analysis of these splice variant cDNAs and their broad mRNA expression in different normal human tissues and cancer cell lines. An upregulation of the splice variant mRNAs expression was observed after HL-60 and erythroblast differentiation. The upregulation of splice variant 4 mRNA was considerably higher than those of the other variants, during erythroid differentiation.
Santos Duarte
Adriana da Silva
Ada S
Centro de Hematologia e Hemoterapia, Universidade Estadual de Campinas, Campinas, SP 13083-970, Brazil.
Traina
Fabíola
F
Favaro
Patricia M Bergamo
PM
Bassères
Daniela Sanchez
DS
de Carvalho
Iara Campos
IC
Medina
Samuel de Souza
Sde S
Costa
Fernando Ferreira
FF
Saad
Sara Teresinha Olalla
ST
D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D017931:DNA Primers; D015870:Gene Expression; D006801:Humans; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012326:RNA Splicing; D000099317:Hippo Kinases
D017931:DNA Primers; D017346:Protein Serine-Threonine Kinases; C430908:STK26 protein, human; D000099317:Hippo Kinases