Casein kinase 2 during early embryonic development in silkworm Bombyx mori: cDNA sequence, gene expression, and enzyme activity.
To clarify the property of casein kinase 2 (CK2) during early embryonic development in the silkworm, we compared the phosphorylation activities of CK2 in non-diapause and diapause eggs until 60 h after oviposition. In nondiapause eggs, the phosphorylated signals were found at each stage and became progressively stronger through each stage. On the other hand, in diapause eggs, the strongest phosphorylated signals were found at approximately 12 to 24 h after oviposition and became progressively weaker through each stage. To clarify the control mechanism of these enzyme activities, we tried to clone cDNAs encoding alpha- and beta-subunit of CK2 and analyze the gene expressions. The deduced amino acid sequence of the isolated cDNAs comprised 342 and 220 residues, and these sequences showed 85-90% identities to the alpha- and beta-subunit of CK2 in Spodoptera frugiperda. RT-PCR indicated that these genes were expressed in nondiapause and diapause eggs. However, these genes expressions were not parallel with the changes in CK2 activity. These results suggest that the changes in CK2 activity are regulated mainly at the level of post-transcription during embryonic development in Bombyx mori.
Yamamoto
Takayuki
T
Biological Laboratory, Center for Natural Science, Kitasato University, Sagamihara, Kanagawa 228-8555, Japan.
Kanekatsu
Motoki
M
Nakagoshi
Motoko
M
Kato
Tomomi
T
Mase
Keisuke
K
Sawada
Hiroshi
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000196:embryology / Q000201:enzymology* / Q000235:genetics*; D047390:Casein Kinase II / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D017344:Genes, Insect; D008969:Molecular Sequence Data; D010063:Ovum / Q000201:enzymology / Q000254:growth & development; D010766:Phosphorylation; D010802:Phylogeny; D021122:Protein Subunits; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D021122:Protein Subunits; D047390:Casein Kinase II