Platelet mitochondrial evaluation during cytochrome c and dichloroacetate treatments of MELAS.
We hypothesized that serial changes in platelet (PLT) mitochondrial enzyme (ME) activities might correspond to the effects of medications for mitochondrial encephalomyopathy and stroke-like episodes (MELAS). Cytochrome c and sodium dichloroacetate (DCA) were given to a 7-year-old girl with MELAS who had an A3243G mitochondrial DNA mutation. The effects were evaluated with whole PLT-ME assays, developed by our group, using a microplate-reader. During cytochrome c treatment, complex II+III (II+III), complex IV (IV) and citrate synthase (CS) activities showed gradual but statistically significant decrease. II+III activity dropped below normal. II+III/CS activity was initially below normal, followed by a transient improvement, then decreased again before the appearance of central nervous system symptoms. II+III, IV, II+III/CS and IV/CS activities reached their lowest levels in association with a stroke-like episode, then increased with DCA treatment. Our results suggest that progressive mitochondrial dysfunction may occur before the stroke-like episodes in MELAS and that DCA treatment may increase mitochondrial activities. Our whole PLT-ME assay system may be useful for serially evaluating mitochondrial functions in relation to clinical symptoms.
Nakano
Kazutoshi
K
Department of Pediatrics, Tokyo Women's Medical University, Shinjuku-ku, Tokyo 162-8666, Japan. knakano@ped.twmu.ac.jp
Tarashima
Mikako
M
Tachikawa
Emiko
E
Noda
Naoko
N
Nakayama
Tomohiro
T
Sasaki
Kaori
K
Mizoguchi
Eriko
E
Matsuzaki
Mihoko
M
Osawa
Makiko
M
D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D045304:Cytochromes c / Q000627:therapeutic use*; D003999:Dichloroacetic Acid / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D017241:MELAS Syndrome / Q000097:blood / Q000188:drug therapy*; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism
D045304:Cytochromes c; D003999:Dichloroacetic Acid