Procollagen alpha 1 type 1: a potential aide in histopathological grading of glioma.
Collagen type I production has been shown to play a role in malignant transformation. We examined procollagen type I expression in brain tumors and with histopathological grading. Expression levels of procollagen alpha 1 type 1 were determined in 5 glioma cell lines by RT-PCR, Northern, and Western blot analysis. In addition, 41 primary brain tumors and 2 metastatic lung cancers to the brain were examined by PCR. Of the 5 glioma cell line analyzed, 3 (glioma 1, SW-1783 and U-118) expressed procollagen alpha 1 type I and were sensitive to vitamin D3 (VD3). In contrast, 2 of the cell lines (U-373 and T-98G) lacked procollagen alpha 1 type 1 expression. In patients' samples, 14 of 15 anaplastic and low grade gliomas expressed procollagen alpha 1 type I, and 12 of the 14 expressed high levels. In contrast, only 12 of 21 high grade gliomas from patients expressed procollagen alpha 1 type1 and among these, only 4 of the 12 expressed high levels. Thus, there is an inversed correlation between procollagen alpha 1 type 1 expression and histopathological grading (R2=- 0.56, p=0.0005). Our data suggest that procollagen alpha 1 type I expression occurs more commonly in intermediate and low grade gliomas and may assist in histopathological grading.
Savaraj
Niramol
N
VA Medical Center, Miami, Florida 33125, USA. nsavaraj@med.miami.edu
Wu
Chunjing
C
Landy
Howard
H
Wangpaijit
Medhi
M
Wei
Yingjie
Y
Kuo
M Tien
MT
Robles
Carlos
C
Furst
A J
AJ
Lampidis
Theodore
T
Feun
Lynn
L
D014408:Biomarkers, Tumor / Q000032:analysis / Q000096:biosynthesis*; D001932:Brain Neoplasms / Q000473:pathology*; D024042:Collagen Type I / Q000032:analysis / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D005910:Glioma / Q000473:pathology*; D006801:Humans; D009367:Neoplasm Staging / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D024042:Collagen Type I