Combination of porphyrins and DNA-alkylation agents: synthesis and tumor cell apoptosis induction.
A series of porphyrin-DNA cross-linking conjugates were synthesized. Their cytotoxicities to tumor cells were tested using MTT assays first. Then, HeLa cell apoptosis induced by these cationic porphyrins under the light was examined by laser confocal microscopy, flow cytometric analysis, and further confirmed by observing the morphological changes and DNA fragmentation mainly.
He
Hanping
H
College of Chemistry and Molecular Sciences, Wuhan University, Hubei, PR China.
Zhou
Yan
Y
Liang
Feng
F
Li
Dongqing
D
Wu
Juanjuan
J
Yang
Li
L
Zhou
Xiang
X
Zhang
Xiaolian
X
Cao
Xiaoping
X
D000478:Alkylation; D017209:Apoptosis / Q000187:drug effects*; D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*; D019800:Phenol / Q000737:chemistry; D011166:Porphyrins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013329:Structure-Activity Relationship
D002412:Cations; D011166:Porphyrins; D000644:Quaternary Ammonium Compounds; D019800:Phenol; D004247:DNA