Entamoeba histolytica sequences and their relationship with experimental liver abscesses in hamsters.
The purpose of the present paper was to analyse the association between sequences of Entamoeba histolytica and their relationship with the development of hepatic abscesses in hamsters, using a complementary DNA library for E. histolytica. From the sequences obtained, we designed oligonucleotides for amplification by PCR. Trophozoites were isolated from faeces of 11 patients in whom cysts from E. histolytica were identified, and these trophozoites were then subjected to monoaxenic culture. Then 1 x 10(5) trophozoites were inoculated into hamster liver, with three hamsters used for every culture. Sequences were obtained for ubiquitin, lectin surface precursor, replication factor MCM3 and surface antigen. The associations between sequences and hepatic abscesses were: 11/11 for ubiquitin, 9/11 for lectin precursor, 4/11 for replication factor and 1/11 for surface antigen. These results suggest that ubiquitin could be an important protein involved in the mechanism of E. histolytica invasion.
Crisóstomo-Vázquez
María Del Pilar
Mdel P
Laboratory of Parasitology Research, Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162 Col. Doctores, 06720 Mexico, DF, Mexico.
Jiménez-Cardoso
Enedina
E
Arroyave-Hernández
Carlos
C
D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D004748:Entamoeba histolytica / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D004749:Entamoebiasis / Q000469:parasitology / Q000473:pathology*; D015723:Gene Library; D006801:Humans; D008101:Liver Abscess, Amebic / Q000469:parasitology / Q000473:pathology*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D025801:Ubiquitin / Q000737:chemistry / Q000235:genetics*
D015800:Protozoan Proteins; D025801:Ubiquitin