title: Salivary thiobarbituric acid reacting substances and malondialdehyde--their relationship to reported smoking and to parodontal status described by the papillary bleeding index.
abstract: BACKGROUND
  Thiobarbituric reacting substances (TBARS) are markers of lipoperoxidation. The best-known specific TBARS is malondialdehyde (MDA). Results from our previous studies have shown that TBARS can be measured in saliva and are increased in patients with gingivitis. Whether MDA is the main TBARS in saliva from patients with altered parodontal status is unknown. Aim. To observe the relationship between the parodontal status and TBARS, MDA and the number of epithelial cells in saliva.
  
  
  METHODS
  In Study I saliva and plasma samples of 15 patients (8F, 7M) suffering from inflammatory periodontal diseases were gathered and TBARS levels were measured in these samples. In Study II saliva samples from 217 consecutive stomatologic patients were collected and analysed for TBARS spectrofluorometrically, MDA by high-performance liquid chromatography and epithelial cell count by light microscopy. Papillary bleeding index (PBI) was determined in standard stomatologic examination.
  
  
  RESULTS
  In Study I results from our previous studies showing no correlation between salivary and plasma TBARS levels were confirmed. This indicates that the local salivary level of TBARS is unlikely to be directly affected by systemic oxidative stress. In Study II higher PBI was associated independently (adjusted for age and sex) tightly with higher TBARS (p<0.001) and with lower number of epithelial cells in saliva (p<0.05). Smokers had higher salivary MDA levels (p<0.003) and lower number of epithelial cells in saliva (p<0.01).
  
  
  CONCLUSIONS
  Salivary TBARS are a simple parameter that partially reflects the parodontal status with a potential usefulness in the clinical stomatology. We show herein that salivary MDA is dependent on age and smoking, but there is no correlation between MDA and PBI. Further studies should uncover the main salivary TBARS compound in patients with altered parodontal status and trace the origin of these salivary lipoperoxidation markers.
authors.affiliation: Faculty of Medicine, Comenius University, Bratislava, Slovakia. petercelec@hotmail.com
authors.forename: Peter
authors.initials: P
authors.lastname: Celec
authors.forename: Július
authors.initials: J
authors.lastname: Hodosy
authors.forename: Viera
authors.initials: V
authors.lastname: Celecová
authors.forename: Ján
authors.initials: J
authors.lastname: Vodrázka
authors.forename: Tomás
authors.initials: T
authors.lastname: Cervenka
authors.forename: Lukác
authors.initials: L
authors.lastname: Halcák
authors.forename: Peter
authors.initials: P
authors.lastname: Bozek
authors.forename: Martin
authors.initials: M
authors.lastname: Kopáni
authors.forename: Matús
authors.initials: M
authors.lastname: Kúdela
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D005891:Gingivitis / Q000276:immunology / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000175:diagnosis; D015227:Lipid Peroxidation; D008054:Lipid Peroxides; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D008875:Middle Aged; D018384:Oxidative Stress; D010510:Periodontal Diseases / Q000378:metabolism* / Q000473:pathology; D017382:Reactive Oxygen Species; D012463:Saliva / Q000378:metabolism*; D012907:Smoking; D013050:Spectrometry, Fluorescence; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*
chemical_list: D008054:Lipid Peroxides; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde