The growth characteristics and ginsenosides isolation of suspension-cultured crown gall of Panax quinquefolium.
The growth characteristics and ginsenosides isolation of the suspension-cultured crown gall of Panax quinquefolium were studied. The result showed that the maximum biomass of cultures was 18.6 g/L (dry weight) and the content of ginsenosides reached its maximum level of 620.4 mg/L on the 27th day. The utilization rates of sugar, phosphorus, nitrogen in NH4+ and nitrogen in NO3- were 91.8%, 100%, 81% and 97%, respectively. Four compounds were isolated from the suspension-cultured crown gall and their structures were elucidated as pseudoginsenoside F11 (I), ginsenoside Rd (II), ginsenoside Rb1 (llI) and ginsenoside Rb3 (IV).
Yu
Rong-Min
RM
Department of Phytochemistry and Pharmacognosy, College of Pharmacy, Jinan University, Guangzhou 510632, China. rongminyu99@hotmail.com
Jin
Qian-Xing
QX
Sun
Hui
H
Ye
Wen-Cai
WC
Zhao
Yu
Y
D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D036145:Ginsenosides / Q000096:biosynthesis / Q000302:isolation & purification*; D005894:Panax / Q000254:growth & development* / Q000378:metabolism*; D010946:Plants, Medicinal / Q000254:growth & development / Q000378:metabolism
D003470:Culture Media; D036145:Ginsenosides