Soybean saponins suppress the release of proinflammatory mediators by LPS-stimulated peritoneal macrophages.
Proinflammatory mediators such as prostaglandins (PGs), nitric oxide (NO), certain inflammatory cytokines and chemokines play a pivotal role in tumor development. The anti-inflammatory properties of naturally occurring phytochemicals are associated with significant reduction in the risk of certain cancers in vitro and in vivo. In the present study, we investigated the effects of soybean saponins on the production of proinflammatory mediators in LPS-stimulated peritoneal macrophages. Soybean saponins significantly inhibited the release of PGE(2), NO, TNFalpha and monocyte chemotactic protein-1 (MCP-1) in a dose-dependent manner. Soybean saponins also down-regulated the expression of COX-2 and iNOS at mRNA/protein levels. Moreover, soybean saponins suppressed NF-kappaB activation by blocking IkappaB-alpha degradation. Our data indicate that soybean saponins exhibit anti-inflammatory properties by suppressing the transcription of inflammatory cytokine genes through the NF-kappaB signaling pathway. The anti-inflammatory properties of soybean saponins may be useful for ameliorating inflammatory diseases as well as suppressing tumor progression.
Kang
Ji-Hye
JH
Department of Food Science and Nutrition, University of Ulsan, Mugeo-dong, Nam-ku, Ulsan 680-749, South Korea.
Sung
Mi-Kyung
MK
Kawada
Teruo
T
Yoo
Hoon
H
Kim
Young-Kyoon
YK
Kim
Jeong-Sang
JS
Yu
Rina
R
D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D020672:I-kappa B Proteins / Q000378:metabolism; D008070:Lipopolysaccharides; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology*; D013025:Glycine max; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D020672:I-kappa B Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; C000606205:Nfkbia protein, mouse; D012503:Saponins; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D015232:Dinoprostone