Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast.
In contrast to animal and fungal cells, green plant cells contain one or multiple chloroplasts, the organelle(s) in which photosynthetic reactions take place. Chloroplasts are believed to have originated from an endosymbiotic event and contain DNA that codes for some of their proteins. Most chloroplast proteins are encoded by the nuclear genome and imported with the help of sorting signals that are intrinsic parts of the polypeptides. Here, we show that a chloroplast-located protein in higher plants takes an alternative route through the secretory pathway, and becomes N-glycosylated before entering the chloroplast.
Villarejo
Arsenio
A
Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, S-90187 Umeå, Sweden.
Burén
Stefan
S
Larsson
Susanne
S
Déjardin
Annabelle
A
Monné
Magnus
M
Rudhe
Charlotta
C
Karlsson
Jan
J
Jansson
Stefan
S
Lerouge
Patrice
P
Rolland
Norbert
N
von Heijne
Gunnar
G
Grebe
Markus
M
Bako
Laszlo
L
Samuelsson
Göran
G
D017360:Arabidopsis / Q000378:metabolism / Q000648:ultrastructure*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000235:genetics; D002736:Chloroplasts / Q000378:metabolism*; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D006031:Glycosylation; D049452:Green Fluorescent Proteins / Q000235:genetics; D008856:Microscopy, Fluorescence; D021382:Protein Sorting Signals; D021381:Protein Transport; D014162:Transfection
D029681:Arabidopsis Proteins; D021382:Protein Sorting Signals; D049452:Green Fluorescent Proteins; D002256:Carbonic Anhydrases