title: First isolation of Japanese encephalitis from Culex quinquefasciatus in Thailand.
abstract: Isolation of Japanese encephalitis (JE) virus using C6/36 cell and immunofluorescence virus antigen detection techniques was attempted from female mosquitoes collected with CDC gravid traps in Samut Songkhram Province in the central region and in Phuket Province in southern Thailand, in 2003. One thousand and eighty female mosquitoes including 6 species of the Culicidae family (Culex quinquefasciatus, Cx. gelidus, Cx. tritaeniorhynchus, Cx. whitmorei, Cx. vishnui complex, Cx. s.g. culiciomyia) (pooled by specific specimen), were processed for virus isolation. Two pools of Cx. quinquefasciatus yielded a JE virus isolation. This represents the first report of JE virus isolation from Cx. quinquefasciatus in Thailand.
authors.affiliation: Center for Vaccine Development, Research Center for Emerging Viral Diseases, Institute of Science and Technology for Research and Development, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Narong
authors.initials: N
authors.lastname: Nitatpattana
authors.forename: Chamnarn
authors.initials: C
authors.lastname: Apiwathnasorn
authors.forename: Philippe
authors.initials: P
authors.lastname: Barbazan
authors.forename: Somjai
authors.initials: S
authors.lastname: Leemingsawat
authors.forename: Sutee
authors.initials: S
authors.lastname: Yoksan
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Gonzalez
mesh_terms: D000818:Animals; D003465:Culex / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D007303:Insect Vectors / Q000821:virology*; D013785:Thailand