title: A 50 Hz sinusoidal magnetic field does not damage MG-63 three-dimensional tumor spheroids but induces changes in their invasive properties.
abstract: The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 1 mT can damage MG-63 osteosarcoma spheroids and induce variations in the invasive properties of these three-dimensional model systems after 2 days of exposure was investigated. Specifically, possible damage induced by these fields was examined by determining changes in spheroid surface morphology (light microscopy), growth (spheroid diameter and protein content determination), lactate dehydrogenase release, and reduced glutathione amount. Possible changes in the invasive properties were studied by invasion chambers. The results show no induction of cell damage by ELF fields while invasion chamber assays demonstrate a significant increase in the invasive potential of exposed spheroids. In order to determine if the fibronectin or hyaluronan receptors are involved, Western blot analysis was conducted on these two proteins. No significant variations were observed in either receptor in MG-63 multicellular tumor spheroids.
authors.affiliation: Dipartimento di Ematologia, Oncologia e Medicina Molecolare, Istituto Superiore di Sanità, Rome, Italy. santini@iss.it
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Santini
authors.forename: Gabriella
authors.initials: G
authors.lastname: Rainaldi
authors.forename: Antonella
authors.initials: A
authors.lastname: Ferrante
authors.forename: Paola
authors.initials: P
authors.lastname: Indovina
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Donelli
authors.forename: Pietro Luigi
authors.initials: PL
authors.lastname: Indovina
mesh_terms: D045744:Cell Line, Tumor / Q000473:pathology / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004560:Electricity / Q000009:adverse effects*; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006801:Humans; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D012516:Osteosarcoma / Q000473:pathology* / Q000503:physiopathology*; D011829:Radiation Dosage; D018874:Spheroids, Cellular / Q000473:pathology* / Q000528:radiation effects*
chemical_list: D009363:Neoplasm Proteins