title: [Analyses of Streptomyces coelicolor inner membrane proteome by multidimentional protein identification technology].
abstract: Streptomyces coelicolor is the model species among streptomycetes. Until now, proteomic analyses of S. coelicolor have been conducted using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry method, few integral membrane proteins were identified due to the hydrophobic and low-abundance nature of these proteins. In this work, 154 possible inner membrane proteins from S. coelicolor were identified using high pH-proteinase K sample preparation method and multidimensional protein identification technology, among them 44 are integral membrane proteins containing at least one transmembrane domain, most peptides and their corresponding proteins were identified experimentally for the first time.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Xuan-Ming
authors.initials: XM
authors.lastname: Shi
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Luo
authors.forename: Gui-Feng
authors.initials: GF
authors.lastname: Zhang
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Su
authors.forename: Yu-Bi
authors.initials: YB
authors.lastname: Huang
authors.forename: Ke-Qian
authors.initials: KQ
authors.lastname: Yang
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry; D016680:Genome, Bacterial / Q000235:genetics; D013058:Mass Spectrometry / Q000379:methods*; D008565:Membrane Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D048372:Streptomyces coelicolor / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D020543:Proteome