title: Synthesis of proteins by native chemical ligation using Fmoc-based chemistry.
abstract: C-terminal peptide alpha-thioesters are valuable intermediates in the synthesis/semisynthesis of proteins by native chemical ligation. They are prepared either by solid-phase peptide synthesis (SPPS) or biosynthetically by protein splicing techniques. The present paper reviews the different methods available for the chemical synthesis of peptide alpha-thioesters using Fmoc-based SPPS.
authors.affiliation: Chemical Biology and Nuclear Sciences Division, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA. camarero1@llnl.gov
authors.forename: Julio A
authors.initials: JA
authors.lastname: Camarero
authors.forename: Alexander R
authors.initials: AR
authors.lastname: Mitchell
mesh_terms: D000596:Amino Acids; D001671:Biochemistry / Q000379:methods*; D004952:Esters / Q000138:chemical synthesis*; D008956:Models, Chemical; D010452:Peptide Biosynthesis; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D019154:Protein Splicing; D011506:Proteins / Q000138:chemical synthesis*
chemical_list: D000596:Amino Acids; D004952:Esters; D010455:Peptides; D011506:Proteins