title: Platinum-based antitumor drugs containing enantiomerically pure alpha-trifluoromethyl alanine as ligand.
abstract: Synthetic amino acids such as fluorinated alpha-amino acids are currently actively investigated for their biological activity. Herein, we report on the synthesis and characterization of platinum complexes containing an N,O-chelated pure enantiomer of alpha-trifluoromethylalanine (alpha-Tfm-Ala). The compounds are either anionic, K[PtCl2(alpha-Tfm-Ala)], or cationic, [PtAm2(alpha-Tfm-Ala)](NO3) (Am2= (NH3)2, ethylendiamine (en), 1,10-phenanthroline (phen), and 2,9-dimethyl-1,10-phenanthroline (Me2phen)). All complexes are highly soluble in water and have been fully characterized by NMR spectroscopy. In vitro cytotoxicity assays on different human tumor cell lines have been performed on some of the isolated compounds. [Pt(NH3)2(alpha-Tfm-Ala)] with R configuration of the amino acid proved to have an activity comparable to that of the reference compound cisplatin. Flow cytometric analysis on NCI-H460 tumor cells (absence of G2/M arrest, which instead is observed in the case of cisplatin) suggests a mechanism of action different from that of cisplatin.
authors.affiliation: Dipartimento Farmaco-Chimico, Università di Bari, Via E. Orabona 4, I-70125 Bari, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Margiotta
authors.forename: Paride
authors.initials: P
authors.lastname: Papadia
authors.forename: Francesco
authors.initials: F
authors.lastname: Lazzaro
authors.forename: Marcello
authors.initials: M
authors.lastname: Crucianelli
authors.forename: Francesco
authors.initials: F
authors.lastname: De Angelis
authors.forename: Claudio
authors.initials: C
authors.lastname: Pisano
authors.forename: Loredana
authors.initials: L
authors.lastname: Vesci
authors.forename: Giovanni
authors.initials: G
authors.lastname: Natile
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D008024:Ligands; D009944:Organoplatinum Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D008024:Ligands; D009944:Organoplatinum Compounds; C507723:alpha-trifluoromethylalanine; D000409:Alanine