Assessment of nociception in acrylamide-induced neuropathy in rats.
Acrylamide was intraperitoneally administered to male Sprague-Dawley rats at four different doses (5, 10, 20 and 30 mg/kg) three times a week for 5 consecutive weeks. Because of motor dysfunction, the 30 mg/kg dose was not used for behavioral pain tests. Clinical status remained good throughout the experiment and no motor deficit was observed at the other doses. We showed that acrylamide administration at low doses and cumulative dose (CD) range of 35-140 mg/kg produced mechanical allodynia and rapid, marked heat (42 degrees C) and cold (10 degrees C) allodynia after tail immersion test. Mechanical and thermal hyperalgesia appeared after higher cumulative doses (70-280 mg/kg), except for cold (4 degrees C) hyperalgesia (20-80 mg/kg). All the modifications persisted throughout all study, except the mechanical hyperalgia. All the cumulative doses tested were lower than those generally reported to induce motor dysfunction (CD>250 mg/kg), confirming that CD may be considered to be a suitable index in assessing neurological signs and suggesting that early detection of acrylamide neurotoxicity would be possible using the sensory tests, especially those for detecting allodynia thresholds.
Ling
Bing
B
Laboratoire de Toxicologie, Faculté de Pharmacie, EA 3848, 28 place Henri-Dunant, BP 38, 63001 Clermont-Ferrand, France Laboratoire de Pharmacologie Médicale, Faculté de Médecine, EA 3848, 28 place Henri-Dunant, BP 38, 63001 Clermont-Ferrand, France Laboratoire de Pharmacologie-Toxicologie, CHU Gabriel-Montpied, 30 place Henri-Dunant, 63001 Clermont-Ferrand, France.
Authier
Nicolas
N
Balayssac
David
D
Eschalier
Alain
A
Coudore
François
F
D020106:Acrylamide / Q000008:administration & dosage*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006941:Hyperesthesia / Q000139:chemically induced / Q000175:diagnosis / Q000503:physiopathology*; D008297:Male; D009437:Neuralgia / Q000139:chemically induced / Q000175:diagnosis / Q000503:physiopathology*; D017288:Pain Threshold / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D020106:Acrylamide