Rheological properties of fast skeletal myosin rod and light meromyosin from walleye pollack and white croaker: contribution of myosin fragments to thermal gel formation.
Myosin rod and light meromyosin (LMM) of walleye pollack and white croaker were examined for their rheological properties by measuring dynamic viscoelastic parameters. Rods from walleye pollack and white croaker increased their storage moduli (G') in the ranges of 29-43 degrees C and 31-38 degrees C, respectively, in temperature sweep analysis. Walleye pollack LMM showed no peak of G' upon heating, whereas the white croaker counterpart exhibited a single sharp peak of G' at 35 degrees C. Loss modulus (G") showed similar temperature-dependent changes for the two fish species as the case of G', irrespective of rod and LMM, although G" values were lower than those of G'. Thus, rheological properties of rod and LMM were different between walleye pollack and white croaker. Taken together with data previously reported for myosin, it was considered that both myosin rods from walleye pollack and white croaker are attributed to thermal gel formation of myosin in a low-temperature range, though in a species-specific manner.
Fukushima
Hideto
H
Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.
Satoh
Yoshie
Y
Yoon
Sung Ho
SH
Togashi
Marie
M
Nakaya
Misako
M
Watabe
Shugo
S
D000818:Animals; D002152:Calorimetry, Differential Scanning; D004548:Elasticity; D048388:Gadiformes; D005782:Gels / Q000737:chemistry; D006358:Hot Temperature; D018482:Muscle, Skeletal / Q000737:chemistry*; D015879:Myosin Subfragments / Q000737:chemistry*; D009218:Myosins / Q000737:chemistry*; D010473:Perciformes; D012212:Rheology; D014783:Viscosity
D005782:Gels; D015879:Myosin Subfragments; D009218:Myosins