[Analysis of RAPD fingerprinting in Exserohilum monoceras strains].
Exserohilium monoseras is a potential agent for barnyardgrass (Echinochlao crus-galli) control. This study was to evaluate the genetic diversity of this fungus with 17 strains isolated from different rice growing regions of China by the random amplified polymorphic DNA (RAPD). Among 25 arbitrary primers, 20 primers could get enough amplified bands for all the strains. A total 239 products were amplified. Polymorphic bands were 95.8% of the total products. The 17 strains could be identified based on RAPD fingerprinting established in this study. Genetic distance calculation and cluster analysis resulted in great genetic variation among the strains. The genetic similarity was related with the morphology and the pathogenicity of the strains. The results showed that RAPD maker could be applied in the utilization and the identification of weed pathogen resources.
Chen
Yong
Y
Department of Agronomy, Quartermaster University of PLA, Changchun 130062, China. chygg@yahoo.com
Ni
Hanwen
H
D001203:Ascomycota / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002681:China; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D031747:Echinochloa / Q000382:microbiology*; D014644:Genetic Variation; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers