Capillary affinity electrophoresis using lectins for the analysis of milk oligosaccharide structure and its application to bovine colostrum oligosaccharides.
Animal colostrum and milk contain complex mixtures of oligosaccharides, which have species-specific profiles. Milk oligosaccharides have various types of structure related to the core structures of glycolipids and N- and O-glycans of glycoproteins and provide a good library to examine the binding of oligosaccharides to various lectins. Recently, we reported a capillary affinity electrophoresis (CAE) method for analyzing the interactions between lectins and complex mixtures of N-linked oligosaccharides prepared from serum glycoproteins. The present paper reports the interactions between 24 milk oligosaccharides and six lectins (PA-I, RCA(120), SBA, WGA, UEA-I, and AAL) analyzed using CAE. Based on the resulting data, we constructed a library that enables us to determine nonreducing terminal monosaccharides, such as Gal, GalNAc, GlcNAc, and Fuc, and to differentiate Gal- or Fuc-linked isomers, such as lacto-N-tetraose, lacto-N-neotetraose, and lacto-N-fucopentaose II and III. In addition, using the library, we show that a combination of the lectins can characterize the neutral oligosaccharides derived from bovine colostrum.
Nakajima
Kazuki
K
Faculty of Pharmaceutical Sciences, Kinki University, Kowakae 3-4-1, Higashiosaka-shi, Osaka 577-8502, Japan.
Kinoshita
Mitsuhiro
M
Matsushita
Namiko
N
Urashima
Tadasu
T
Suzuki
Minoru
M
Suzuki
Akemi
A
Kakehi
Kazuaki
K
D000818:Animals; D002417:Cattle; D003126:Colostrum / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013997:Time Factors
D037102:Lectins; D009844:Oligosaccharides