Ophiostoma dentifundum sp. nov. from oak in Europe, characterized using molecular phylogenetic data and morphology.
Previous phylogenetic studies based on ITS sequence data have shown that Ophiostoma species with Sporothrix anamorphs include several species complexes. Isolates from oak in Poland and Hungary, which have previously been referred to as O. stenoceras, as well as isolates morphologically similar to S. inflata formed the basis of this study. Identification was based on sequences for the ITS region of rDNA operon and partial beta-tubulin gene. Analyses showed that isolates from Poland and Hungary reside in a well resolved clade, separate from those in the O. stenoceras-complex. The morphology of these isolates was compared with those of strains in the O. stenoceras complex and S. inflata. Morphological differences in teleomorph and anamorph structures were found between the isolates from Poland and Hungary and those in the O. stenoceras-complex. Growth characteristics and the presence of the teleomorph in culture could be used to separate this fungus from isolates in the S. inflata-complex. The fungus from Poland and Hungary is described here as O. dentifundum sp. nov. It is phylogenetically most closely related to isolates of S. inflata, which represent four well defined groups based on morphology and DNA sequence phylogeny.
Aghayeva
Dilzara N
DN
Forestry and Agricultural Biotechnology Institute, University of Pretoria, South Africa. adilzara@hotmail.com
Wingfield
Michael J
MJ
Kirisits
Thomas
T
Wingfield
Brenda D
BD
D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D006814:Hungary; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D011044:Poland; D016133:Polymerase Chain Reaction; D029963:Quercus / Q000382:microbiology*; D012340:RNA, Ribosomal, 5.8S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D013175:Sporothrix / Q000235:genetics; D014404:Tubulin / Q000737:chemistry / Q000235:genetics
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S; D014404:Tubulin