title: Sub-micron texturing for reducing platelet adhesion to polyurethane biomaterials.
abstract: Platelet adhesion is a key event in thrombus development on blood-contacting medical devices. It has been demonstrated that changes to the chemistry of a material surface can reduce platelet adhesion. In this work, it is hypothesized that sub-micron surface textures may also reduce adhesion via a decrease in the surface area of material with which platelets can make contact, and hence a decreased probability of interaction with adhesive ligands. A polyether(urethane urea) was textured with two different sizes of sub-micron pillars using a replication molding technique that did not alter the material surface chemistry. Adhesion of platelets was assessed in a physiologically relevant shear stress range of 0-67 dyn/cm2 using a rotating disk system. Platelets were immunofluorescently labeled and adhesion was compared on smooth and textured samples. Platelet adhesion was greatest at low shear stress ranging from 0 to 5 dyn/cm2, and sub-micron textures were observed to reduce platelet adhesion in this range. Additionally, non-adherent platelets did not demonstrate large-scale activation after exposure to textured samples. We conclude that surface textures with sub-platelet dimensions may reduce platelet adhesion from plasma to polyether(urethane urea) at low shear stress.
authors.affiliation: Department of Surgery, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA.
authors.forename: Keith R
authors.initials: KR
authors.lastname: Milner
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Snyder
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Siedlecki
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001792:Blood Platelets / Q000502:physiology / Q000648:ultrastructure; D002417:Cattle; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning; D010973:Platelet Adhesiveness / Q000502:physiology; D011140:Polyurethanes / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D011140:Polyurethanes