Detection of homocysteine and cysteine.
At elevated levels, homocysteine (Hcy, 1) is a risk factor for cardiovascular diseases, Alzheimer's disease, neural tube defects, and osteoporosis. Both 1 and cysteine (Cys, 3) are linked to neurotoxicity. The biochemical mechanisms by which 1 and 3 are involved in disease states are relatively unclear. Herein, we describe simple methods for detecting either Hcy or Cys in the visible spectral region with the highest selectivity reported to date without using biochemical techniques or preparative separations. Simple methods and readily available reagents allow for the detection of Cys and Hcy in the range of their physiologically relevant levels. New HPLC postcolumn detection methods for biological thiols are reported. The potential biomedical relevance of the chemical mechanisms involved in the detection of 1 is described.
Wang
Weihua
W
Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
Rusin
Oleksandr
O
Xu
Xiangyang
X
Kim
Kyu Kwang
KK
Escobedo
Jorge O
JO
Fakayode
Sayo O
SO
Fletcher
Kristin A
KA
Lowry
Mark
M
Schowalter
Corin M
CM
Lawrence
Candace M
CM
Fronczek
Frank R
FR
Warner
Isiah M
IM
Strongin
Robert M
RM
D000447:Aldehydes / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D003545:Cysteine / Q000097:blood* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006710:Homocysteine / Q000097:blood* / Q000737:chemistry; D006801:Humans; D010455:Peptides / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D028561:Transition Elements / Q000737:chemistry
D000447:Aldehydes; D005456:Fluorescent Dyes; D010455:Peptides; D013438:Sulfhydryl Compounds; D013844:Thiazoles; D028561:Transition Elements; D006710:Homocysteine; D003545:Cysteine