title: Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: relevance for natural killer-dendritic cell interaction.
abstract: In this study, we demonstrate the involvement of DNAM-1-triggering receptor and its ligands, poliovirus receptor (PVR) and Nectin-2, in natural killer (NK) cell-mediated lysis of dendritic cells (DCs). The surface expression of both ligands was up-regulated in DCs as compared to monocytes. It reached maximal densities after DC maturation induced by different stimuli including lipopolysaccharide (LPS), poly I:C, flagellin, and CD40L. Both immunohistochemical analysis and confocal microscopy revealed expression of DNAM-1 ligands by DCs in lymph nodes in which they were localized in the parafollicular T-cell region and surrounded the high endothelial venules. Remarkably, in cytolytic assays, DNAM-1 cooperated with NKp30 in the NK-mediated killing of both immature and mature DCs and the degree of contribution of DNAM-1 appeared to correlate with the surface densities of its specific ligands PVR and Nectin-2.
authors.affiliation: Istituto Nazionale per la Ricerca sul Cancro, L.go R. Benzi 10, 16132 Genova, Italy. daniela.pende@istge.it
authors.forename: Daniela
authors.initials: D
authors.lastname: Pende
authors.forename: Roberta
authors.initials: R
authors.lastname: Castriconi
authors.forename: Paola
authors.initials: P
authors.lastname: Romagnani
authors.forename: Grazia Maria
authors.initials: GM
authors.lastname: Spaggiari
authors.forename: Stefania
authors.initials: S
authors.lastname: Marcenaro
authors.forename: Alessandra
authors.initials: A
authors.lastname: Dondero
authors.forename: Elena
authors.initials: E
authors.lastname: Lazzeri
authors.forename: Laura
authors.initials: L
authors.lastname: Lasagni
authors.forename: Stefania
authors.initials: S
authors.lastname: Martini
authors.forename: Paola
authors.initials: P
authors.lastname: Rivera
authors.forename: Andrea
authors.initials: A
authors.lastname: Capobianco
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Moretta
authors.forename: Alessandro
authors.initials: A
authors.lastname: Moretta
authors.forename: Cristina
authors.initials: C
authors.lastname: Bottino
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D023201:CD40 Ligand / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D053646:Interleukin-2 Receptor beta Subunit; D007694:Killer Cells, Natural / Q000276:immunology*; D008024:Ligands; D008070:Lipopolysaccharides / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D055628:Natural Cytotoxicity Triggering Receptor 3; D011070:Poly I-C / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000276:immunology*; D011991:Receptors, Virus / Q000276:immunology*; D015854:Up-Regulation / Q000276:immunology; D000098862:T Lineage-Specific Activation Antigen 1
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; C508020:IL2RB protein, human; D053646:Interleukin-2 Receptor beta Subunit; D008024:Ligands; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C431710:NCR3 protein, human; D055628:Natural Cytotoxicity Triggering Receptor 3; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D011991:Receptors, Virus; C066100:poliovirus receptor; D023201:CD40 Ligand; D011070:Poly I-C