title: In vitro study of the interaction of polyalkilimide and polyvinyl alcohol hydrogels with cells.
abstract: Hydrogels are a class of polymers that in the last decade have had a great development and application for soft tissue augmentation, due to their similarity to this tissue for their high water content. The in vitro effects of polyalkylmide hydrogel (pAI) and polyvinyl alcohol hydrogel (pVOH) on human lymphocytes and U937 cells viability, apoptosis and cell shape were investigated. Cell viability was always higher than 70%, thus showing the hydrogels were not cytotoxic for both cell lines. Some differences were, however, found. At short time, lymphocytes were very sensitive to the hydrogels incubation, while at long time, U937 cells were the most sensitive cells. Other differences on cell viability were related to the time of incubation, to the type of hydrogel and to the polymers concentration. Cell viability decreased only at the longest time of incubation and with the highest hydrogel concentration. Accordingly, cell death by apoptosis increased; necrosis was never observed in the cultures. Concentration- and hydrogel-dependent modifications of cell shape (bigger cell volume, elongations of cells) were observed in a few percentage of viable cells. In conclusion, the very high in vitro degree of biocompatibility shown by both hydrogels encourages their use as dermal fillers.
authors.affiliation: Department of Biological and Environmental Science and Technologies (DiSTeBA), University of Lecce, Via per Monteroni, 73100 Lecce, Italy. luciana.dini@unile.it
authors.forename: L
authors.initials: L
authors.lastname: Dini
authors.forename: E
authors.initials: E
authors.lastname: Panzarini
authors.forename: M A
authors.initials: MA
authors.lastname: Miccoli
authors.forename: V
authors.initials: V
authors.lastname: Miceli
authors.forename: C
authors.initials: C
authors.lastname: Protopapa
authors.forename: P A
authors.initials: PA
authors.lastname: Ramires
mesh_terms: D000180:Acrylic Resins / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002452:Cell Count; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020100:Hydrogels / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D018625:Microscopy, Atomic Force; D009000:Monocytes / Q000166:cytology; D011108:Polymers / Q000494:pharmacology*; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D013997:Time Factors; D020298:U937 Cells
chemical_list: D000180:Acrylic Resins; D020100:Hydrogels; D011108:Polymers; C062364:polyvinyl alcohol hydrogel; C006903:carbopol 940; D011142:Polyvinyl Alcohol