Electroporation for targeted gene transfer.
The utilisation of nonviral gene delivery methods has been increasing steadily, however, a drawback has been the relative low efficiency of gene transfer with naked DNA compared with viral delivery methods. In vivo electroporation, which has previously been used clinically to deliver chemotherapeutic agents, also enhances the delivery of plasmid DNA and has been used to deliver plasmids to several tissue types, particularly muscle and tumour. Recently, a large number of preclinical studies for a variety of therapeutic modalities have demonstrated the potential of electrically mediated gene transfer. Although clinical trials using gene transfer with in vivo electroporation have not as yet been realised, the tremendous growth of this technology suggests that the first trials will soon be initiated.
Heller
Loree C
LC
University of South Florida, Center of Molecular Delivery, Department of Medical Microbiology and Immunology, Center for Molecular Delivery, College of Medicine, Tampa, 33612-4799, USA. rheller@hsc.usf.edu
Ugen
Kenneth
K
Heller
Richard
R
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D018274:Electroporation; D004921:Erythropoietin / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D005544:Forecasting; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods / Q000639:trends*; D006133:Growth Substances / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D006402:Hematologic Diseases / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D006801:Humans; D007273:Injections, Intramuscular; D018664:Interleukin-12 / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D008545:Melanoma / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D011488:Protein Deficiency / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D014118:Toxins, Biological / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism
D006133:Growth Substances; D014118:Toxins, Biological; D019444:Vaccines, DNA; D004921:Erythropoietin; D018664:Interleukin-12