Voltage-dependent ion channels in the mouse RPE: comparison with Norrie disease mice.
We studied electrophysiological properties of cultured retinal pigment epithelial (RPE) cells from mouse and a mouse model for Norrie disease. Wild-type RPE cells revealed the expression of ion channels known from other species: delayed-rectifier K(+) channels composed of Kv1.3 subunits, inward rectifier K(+) channels, Ca(V)1.3 L-type Ca(2+) channels and outwardly rectifying Cl(-) channels. Expression pattern and the ion channel characteristics current density, blocker sensitivity, kinetics and voltage-dependence were compared in cells from wild-type and Norrie mice. Although no significant differences were observed, our study provides a base for future studies on ion channel function and dysfunction in transgenic mouse models.
Wollmann
Guido
G
Institut fuer Klinische Physiologie, Charite-Universitaetsmedizin Berlin, Campus Benjamin Franklin, Germany. guido.wollmann@yale.edu
Lenzner
Steffen
S
Berger
Wolfgang
W
Rosenthal
Rita
R
Karl
Mike O
MO
Strauss
Olaf
O
D000818:Animals; D015153:Blotting, Western; D020746:Calcium Channels, L-Type / Q000378:metabolism; D002478:Cells, Cultured; D018118:Chloride Channels / Q000378:metabolism; D004195:Disease Models, Animal; D015785:Eye Diseases, Hereditary / Q000378:metabolism*; D040181:Genetic Diseases, X-Linked / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D051664:Kv1.3 Potassium Channel / Q000378:metabolism; D008564:Membrane Potentials; D051379:Mice; D018345:Mice, Knockout; D018408:Patch-Clamp Techniques; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism
C485995:Cacna1d protein, mouse; D020746:Calcium Channels, L-Type; D018118:Chloride Channels; D007473:Ion Channels; D051664:Kv1.3 Potassium Channel; D024661:Potassium Channels, Inwardly Rectifying