South African GB virus C isolates: interactions between genotypes 1 and 5 isolates and HIV.
GB virus C (GBV-C) is a common, apparently nonpathogenic human flavivirus that has been associated in some studies with prolonged survival in human immunodeficiency virus (HIV)-positive persons. There are 5 distinct GBV-C genotypes localized to specific geographic regions, and genotype 2 has been previously shown to inhibit HIV replication in vitro in lymphocyte cultures. We identified GBV-C virus isolates representing genotypes 1, 2, and 5 in samples from South African blood donors. GBV-C genotype 1 and 5 isolates replicated in lymphocyte culture, inhibited X4 and R5 HIV-1 isolates, and induced RANTES and stromal-derived factor-1 chemokines in vitro. Thus, African GBV-C genotypes can inhibit HIV replication in vitro.
Xiang
Jinhua
J
Research and Medical Service, Iowa City VA Medical Center, Iowa City, 52242, USA.
Sathar
M Aslam
MA
McLinden
James H
JH
Klinzman
Donna
D
Chang
Qing
Q
Stapleton
Jack T
JT
D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics; D001782:Blood Donors; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000032:analysis; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000032:analysis; D018178:Flaviviridae Infections / Q000453:epidemiology* / Q000821:virology; D029407:GB virus C / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013019:South Africa / Q000453:epidemiology; D014762:Viral Interference; D014766:Viremia / Q000453:epidemiology; D014779:Virus Replication
D020121:5' Untranslated Regions; C515255:CXCL12 protein, human; D018946:Chemokine CCL5; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D016655:HIV Core Protein p24