title: Urinary protein analysis in pre- and postoperative cancer patients.
abstract: Urinary proteins from six patients with esophageal cancer and two with stomach cancer were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analyses were performed on days-1 to 3, 5, 7, 10, 14, and 21 (or 22) after surgery. The protein patterns were scanned by densitometry and divided into nine fractions. The main proteins in the fractions (Fr.) were identified as follows: immunoglobulin G in Fr. A, Tamm-Horsfall glycoprotein (THP) in Fr. B, transferrin in Fr. C, albumin in Fr. D, alpha(1)-acid glycoprotein in Fr. E, alpha(1)-microglobulin in Fr. F, retinol binding protein in Fr. G, and beta(2)-microglobulin in Fr. I. The protein in Fr. H was not identified. The percentage of each fraction was calculated from the densitometry pattern of each lane. The percentage values were averaged among all the patients, and pre- and postoperative data were compared. The percentage of Frs. E, F, and G increased on days 1-7, and the changes in these three proteins were similar to changes in serum C-reactive protein (CRP). In particular, the percentage of Fr. G peaked within 1 day of operation, which was faster than for CRP. Conversely, other fractions decreased. These results suggest that urinary protein analysis is useful for monitoring the response to surgical stress.
authors.affiliation: Analytical Laboratory Chemistry, Graduate School of Allied Health Sciences, Tokyo Medical and Dental University, Japan. kayo.s.mtec@tmd.ac.jp
authors.forename: Kayo
authors.initials: K
authors.lastname: Yokomizo
authors.forename: Shiro
authors.initials: S
authors.lastname: Iijima
authors.forename: Nobue
authors.initials: N
authors.lastname: Sakai
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Kurihara
authors.forename: Nobuo
authors.initials: N
authors.lastname: Hitratsuka
authors.forename: Kagami
authors.initials: K
authors.lastname: Nagai
authors.forename: Takehisa
authors.initials: T
authors.lastname: Iwai
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Shiba
mesh_terms: D000368:Aged; D002097:C-Reactive Protein / Q000032:analysis; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000601:surgery*; D006801:Humans; D007674:Kidney Diseases / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D011182:Postoperative Care; D011300:Preoperative Care; D011507:Proteinuria / Q000652:urine*
chemical_list: D002097:C-Reactive Protein