title: Involvement of dorsal column nucleus neurons in nociceptive transmission in aged rats.
abstract: To clarify the functional role of the dorsal column nucleus (DCN) in nociception in rats with advancing age, single neuronal activity and substance P-like immunoreactivity (SP-LI) of the gracile nucleus (GN) were studied in aged rats (29 to 34 mo old) and adult rats (9 to 12 mo old). A total of 122 neurons [aged: 34 wide-dynamic-range (WDR), two nociceptive-specific (NS), and 32 low-threshold mechanical (LTM) neurons; adult: 22 WDR and 32 LTM neurons] were recorded from GN. For WDR neurons, the latency to antidromic activation of the ventral posterior lateral nucleus of the thalamus showed no difference between the aged and adult rats. Sciatic nerve stimulation with C-fiber intensity induced responses of GN with significantly longer latency in aged rats than in adults, whereas there was no difference in the response latency to A-fiber intensity stimulation. Background activity and afterdischarges were significantly higher in the aged rats than those in the adult rats. Responses to noxious mechanical and thermal stimuli were significantly greater in the aged rats during application of graded stimuli. There were no significant differences in responses to nonnoxious mechanical stimulus, mechanical response threshold, and the size of the receptive fields between neurons in the aged and adult rats. The area occupied by SP-LI fibers in the GN and the size of SP-LI dorsal root ganglia neurons were significantly larger in aged rats than in adults. The present findings suggest that the hyperexcitability of GN neurons could be involved in abnormal noxious pain sensations with advancing age.
authors.affiliation: Department of Physiology, School of Dentistry, Nihon University, Chiyoda-ku, Tokyo, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Kitagawa
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Tsuboi
authors.forename: Akiko
authors.initials: A
authors.lastname: Ogawa
authors.forename: Ke
authors.initials: K
authors.lastname: Ren
authors.forename: Suzuro
authors.initials: S
authors.lastname: Hitomi
authors.forename: Kimiko
authors.initials: K
authors.lastname: Saitoh
authors.forename: Osamu
authors.initials: O
authors.lastname: Takahashi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Masuda
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Harada
authors.forename: Naoki
authors.initials: N
authors.lastname: Hanzawa
authors.forename: Kenro
authors.initials: K
authors.lastname: Kanda
authors.forename: Koichi
authors.initials: K
authors.lastname: Iwata
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000367:Age Factors; D000375:Aging / Q000502:physiology*; D000818:Animals; D001931:Brain Mapping; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods; D005071:Evoked Potentials / Q000502:physiology / Q000528:radiation effects; D007150:Immunohistochemistry / Q000379:methods; D009474:Neurons / Q000145:classification / Q000502:physiology*; D010146:Pain / Q000209:etiology / Q000503:physiopathology*; D010147:Pain Measurement / Q000379:methods; D017288:Pain Threshold / Q000502:physiology / Q000528:radiation effects; D010812:Physical Stimulation / Q000379:methods; D051381:Rats; D011916:Rats, Inbred F344; D011930:Reaction Time / Q000502:physiology / Q000528:radiation effects; D013116:Spinal Cord / Q000166:cytology*; D013373:Substance P / Q000378:metabolism; D013787:Thalamic Nuclei / Q000502:physiology / Q000528:radiation effects; D013997:Time Factors
chemical_list: D013373:Substance P