Alternative estrogen receptors homologous to classical receptor alpha in murine neural tissues.
Although it is widely accepted the existence of putative estrogen receptors (ERs) localized at extranuclear domains in the brain, their molecular identity is still unclear. We have previously demonstrated in a murine septal cell line the existence of a membrane-related ER (mER) that participates in estrogen-mediated neuroprotection. To investigate the molecular structure of mER, we have used a battery of antibodies raised against different domains of the classical ERalpha to immunoblot with plasma membrane fractions from septal SN56 and hippocampal HT22 cell lines, and microsomal fractions of mouse septal and hippocampal tissues. The results confirmed that mER is the homologue of its intracellular counterpart ERalpha, suggesting the possibility that both nuclear and extranuclear receptors may share a common origin.
Marin
Raquel
R
Laboratory of Cellular Neurobiology, Department of Physiology, School of Medicine, University of La Laguna, Sta. Cruz de Tenerife, Spain. rmarin@ull.es
Ramírez
Cristina M
CM
González
Miriam
M
Alonso
Rafael
R
Díaz
Mario
M
D000818:Animals; D002460:Cell Line; D047628:Estrogen Receptor alpha / Q000032:analysis / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D008861:Microsomes / Q000378:metabolism; D020033:Protein Isoforms / Q000032:analysis / Q000378:metabolism; D011960:Receptors, Estrogen / Q000032:analysis / Q000378:metabolism*; D020665:Septum of Brain / Q000378:metabolism*; D014018:Tissue Distribution
D047628:Estrogen Receptor alpha; D020033:Protein Isoforms; D011960:Receptors, Estrogen