Biodegradable honeycomb-patterned film composed of poly(lactic acid) and dioleoylphosphatidylethanolamine.
Honeycomb-patterned films have been reported to be useful for scaffolds of cell culture in tissue engineering. In the present study, we investigated a new compound, dioleoylphosphatidylethanolamine (DOPE), a naturally derived phospholipid having unsaturated fatty acid moieties, as a surfactant for fabricating honeycomb-patterned poly(d,l-lactide) (PLA) film. Only DOPE among commercially available phospholipids was useful as a surfactant, and it showed good solubility in PLA/chloroform solution and an excellent property for fabricating honeycomb-patterned film (the concentration of DOPE was from 0.2% to 20% by weight based on the weight of PLA). The pore size of the honeycomb was uniform, and all pores were interconnected with each other. The contact angle of water on the honeycomb-patterned film was affected by the amount of DOPE. Time-of-flight secondary ion mass spectrometer (TOF-SIMS) data suggested that DOPE was concentrated on the surface of the honeycomb-patterned film. To investigate cell proliferation and adhesion on the honeycomb-patterned film, NIH3T3 fibroblast cells were cultured on the film. The NIH3T3 cells adhered well on the honeycomb-patterned PLA film with DOPE (PLA-DOPE) and showed good cell proliferation compared to that on honeycomb-patterned PLA film fabricated with a copolymer (CAP) of dodecylacrylamide and omega-carboxyhexylacrylamide (PLA-CAP). These results suggest that the honeycomb-patterned PLA-DOPE can be applicable as a scaffold for cells with better profiles in comparison with PLA-CAP.
Fukuhira
Yukako
Y
Department of Tissue Engineering Development, Innovation Research Institute, Teijin Ltd., 4-3-2, Asahigaoka, Hino, Tokyo 191-8512, Japan. y.fukuhira@teijin.co.jp
Kitazono
Eiichi
E
Hayashi
Takami
T
Kaneko
Hiroaki
H
Tanaka
Masaru
M
Shimomura
Masatsugu
M
Sumi
Yoshihiko
Y
D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D001673:Biodegradation, Environmental; D002448:Cell Adhesion; D049109:Cell Proliferation; D019344:Lactic Acid / Q000737:chemistry*; D013058:Mass Spectrometry; D051379:Mice; D041681:NIH 3T3 Cells; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D012995:Solubility; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*
D001672:Biocompatible Materials; D010714:Phosphatidylethanolamines; D011091:Polyesters; D011108:Polymers; D013501:Surface-Active Agents; C094877:dioleoyl phosphatidylethanolamine; D019344:Lactic Acid; C033616:poly(lactide)