[Ca2+ signaling pathways associated with the invasion of Streptococcus pneumoniae to type II pneumocytes].
Labelled F-actin with FITC-phalloidin, we observed F-actin rearrangements by Streptococcus pneumoniae adhesion of type II pneumocytes (A549). Invasion of S. pneumoniae to A549 cells was determined by pretreating A549 cells with cytochalasin D. To investigate whether F-actin rearrangements can be blocked by Ca2+ inhibitors, A549 cells were pretreated with Ca2+ inhibitors datrollene. A549 cells were loaded with Fura-2/AM to determine the concentration of cytosolic free calcium by S. pneumoniae adhesion of A549 cells after 30,60,90 minutes respectively. Intact S. pneumoniae can promote F-actin rearrangements. Cytochalasin D is able to prevent S. pneumoniae invasion of A49 cells. S. pneumoniae adhesion of A549 cells increased cytosolic free calcium after 30, 60, 90 minuts. Ca2+ inhibitors datrollene block F-actin rearrangements dose dependently. It suggested S. pneumoniae can provoke F-actin rearrangements through Ca2+ signaling pathways, which will further lead to S. pneumoniae invasion of A49 cells.
Yin
Yibing
Y
Department of Clinical Biochemistry, Faculty of Laboratory Medicine, Chongqing University of Medical sciences, Chongqing 400016, China. yibingyin@21cn.com
Xu
Banglao
B
Luo
Jinyong
J
Zhu
Dan
D
Meng
Jiangping
J
D000199:Actins / Q000378:metabolism; D001422:Bacterial Adhesion / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D002460:Cell Line; D015638:Cytochalasin D / Q000494:pharmacology; D006801:Humans; D011008:Pneumococcal Infections / Q000378:metabolism / Q000382:microbiology*; D020545:Respiratory Mucosa / Q000378:metabolism / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000502:physiology*
D000199:Actins; D015638:Cytochalasin D; D002118:Calcium