Intein-mediated, in vitro and in vivo protein modifications with small molecules.
We review intein-mediated approaches for the site-specific modifications of proteins and highlight their applications in (1) the site-specific in vitro and in vivo biotinylation of proteins for protein arrays and (2) the site-specific in vivo labeling of proteins in living cells.
Tan
Lay Pheng
LP
Department of Biological Sciences, National University of Singapore, 3, Science Drive 3, Singapore 117543, Republic of Singapore.
Yao
Shao Q
SQ
D001710:Biotin / Q000737:chemistry; D019921:Biotinylation / Q000379:methods; D002474:Cell-Free System; D049452:Green Fluorescent Proteins / Q000737:chemistry; D047668:Inteins / Q000502:physiology*; D040081:Protein Array Analysis / Q000379:methods; D015202:Protein Engineering / Q000379:methods; D046188:Protein Modification, Translational / Q000502:physiology*; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods
D011506:Proteins; D049452:Green Fluorescent Proteins; D001710:Biotin