Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens.
Intestinal colonization by enteropathogenic and enterohemorrhagic Escherichia coli requires the locus of enterocyte effacement-encoded type III secretion system. We report that NleC and NleD are translocated into host cells via this system. Deletion mutants induced attaching and effacing lesions in vitro, while infection of calves or lambs showed that neither gene was required for colonization.
Marchés
Olivier
O
CMMI, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom.
Wiles
Siouxsie
S
Dziva
Francis
F
La Ragione
Roberto M
RM
Schüller
Stephanie
S
Best
Angus
A
Phillips
Alan D
AD
Hartland
Elizabeth L
EL
Woodward
Martin J
MJ
Stevens
Mark P
MP
Frankel
Gad
G
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D020895:Enterocytes / Q000378:metabolism* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D008969:Molecular Sequence Data; D009154:Mutation; D021381:Protein Transport; D017422:Sequence Analysis, DNA; D012756:Sheep
D029968:Escherichia coli Proteins; C506688:Z0986 protein, E coli; C506689:Z0990 protein, E coli