Serological survey of Babesia bovis, Babesia bigemina, and Anaplasma marginale antibodies in cattle from the semi-arid region of the state of Bahia, Brazil, by enzyme-linked immunosorbent assays.
The objectives of this work were to determine the prevalence of Babesia bovis, Babesia bigemina, and Anaplasma marginale detecting antibodies in cattle raised in the semi-arid region of the state of Bahia, Brazil, through indirect enzyme linked immunosorbent assays (ELISA) and to compare the performances of indirect enzyme-linked immunosorbent assays with crude (I-ELISA-CrAnaAg) and recombinant major surface protein-5 (I-ELISA-MSP-5Ag), as antigens to detect antibodies against A. marginale. An stable enzootic area was found in Senhor do Bonfim and Euclides da Cunha for B. bovis that showed 86 and 95.5% of prevalence, respectively, and for B. bigemina with 90.8 and 91.3%. On the other hand, Uauá and Juazeiro, were characterized as enzootically unstable areas, since prevalences were: B. bovis--63.7 and 56.4% and B. bigemina--53 and 54.8%, respectively. The prevalence of A. marginale in the four municipalities was above 97% with I-ELISA-CrAnaAg and 94.8% with I-ELISA-MSP-5Ag which is an indication of stable enzootic condition for the rickettsia. The I-ELISA-CrAnaAg and I-ELISA-MSP-5Ag showed a highly significant association (r = 0.977), which means that both ELISA tests are suitable for epidemiological studies of A. marginale.
Barros
Silvia L
SL
Departamento de Patologia e Clínica Veterinária, Escola de Medicina Veterinária, Universidade Federal da Bahia, Salvador, BA, Brasil.
Madruga
Claudio R
CR
Araújo
Flabio R
FR
Menk
Carlos F
CF
de Almeida
Maria Angela O
MA
Melo
Elaine P S
EP
Kessler
Raul H
RH
D042322:Anaplasma marginale / Q000276:immunology*; D000712:Anaplasmosis / Q000453:epidemiology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000913:Antibodies, Protozoan / Q000097:blood*; D001403:Babesia / Q000276:immunology*; D016793:Babesia bovis / Q000276:immunology; D001404:Babesiosis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015995:Prevalence; D016036:Seroepidemiologic Studies
D000907:Antibodies, Bacterial; D000913:Antibodies, Protozoan