title: Inteins and affinity resin substitutes for protein purification and scale up.
abstract: The development of self-cleaving fusion-tag technology has greatly simplified the purification of recombinant proteins at laboratory scale. The self-cleaving capability of these tags has recently been combined with additional purification tags to generate novel and convenient protein purification methods at a variety of scales. In this review, we describe some of these methods, and provide a rudimentary economic analysis of hypothetical large-scale applications. This work is expected to provide a rough outline for the evaluation of these methods for large-scale bioprocessing of a variety of products.
authors.affiliation: Department of Chemical Engineering, A213 E-QUAD, Princeton University, Princeton, NJ 08544, USA. reza@princeton.edu
authors.forename: Mahmoud Reza
authors.initials: MR
authors.lastname: Banki
authors.forename: David W
authors.initials: DW
authors.lastname: Wood