title: [Comparative study on the resting habit of Anopheles minimus A and Anopheles minimus C in Yunnan Province].
abstract: OBJECTIVE
  To compare the difference of resting habitat between Anopheles minimus A and An. minimus C in Yunnan Province.
  
  
  METHODS
  A village was selected in Mengla and Yuanjiang County respectively to collect mosquitoes by hanging ultraviolet lamps inside the cattle pen and human dwellings. The samples were identified by multiplex-PCR after morphological examination. Suspicious An. minimus A/C hybrid samples were identified by PCR-ASA, PCR-RFLP and D3 region sequence.
  
  
  RESULTS
  In human dwellings, the proportion of An. minimus A and C was 21.35% and 78.65%, and in cattle pen, 28.54% and 71.46% respectively. As for the An. minimus A/C hybrid, the result of PCR-ASA and PCR-RFLP displayed the pattern of both An. minimus A and An. minimus C (PCR-ASA: 376, 294 and 112 bp, PCR-RFLP:108, 268 and 376 bp), the D3 sequence displayed heterozygosis in the variation place of An. minimus A and An. minimus C, which means that the hybrid possesses the signal of both An. minimus A and An. minimus C.
  
  
  CONCLUSIONS
  There is no resting habit difference between An. minimus A and An. minimus C in Yunnan Province. In Mengla County, 5 An. minimus A/C hybrids have been found for the first time.
authors.affiliation: National Institute of Parasitic Diseases, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zheng
authors.forename: Lin-Hua
authors.initials: LH
authors.lastname: Tang
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Ma
authors.forename: Wen-Qi
authors.initials: WQ
authors.lastname: Shi
authors.forename: Shui-Sen
authors.initials: SS
authors.lastname: Zhou
authors.forename: Xue-Zhong
authors.initials: XZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D002681:China; D006579:Heterozygote; D016133:Polymerase Chain Reaction; D013045:Species Specificity