title: Analysis of evolutionary conservation in CD1d molecules among primates.
abstract: The hereditary conservation in the genetically encoded CD1D sequences of various primates was analyzed. Genomic CD1D sequences of 17 rhesus macaques with distinct origins, eight Indian and nine Chinese, were examined and differences of only one or two nucleotides were detected and the consensus sequence of rhesus CD1D was determined. CD1D consensus sequences of three African green monkeys (AGMs) and the rhesus monkeys were then compared to study the evolutionary differences among interspecies. The CD1D consensus sequence determined from AGMs apparently differed by seven nucleotides from the rhesus consensus sequence, and nucleotide difference induced only three amino acid changes within Exon3, corresponding to the alpha2 domain of CD1d having a hydrophobic ligand-binding pocket. Such changes in the alpha2 domain may alter the characteristics of the SIV-derived glycolipid/lipid antigens presented by each CD1d molecule to innate natural killer T cells. In addition, the CD1D genomic sequences of three chimpanzees (chimps) were determined. To our surprise, although Exon2 and Exon3 reflecting antigen-binding alpha1 and alpha2 domains in chimps' CD1D were identical to that in humans except one amino acid, three amino acids within Exon4, reflecting alpha3 domain, were distinct from humans, and one of them was identical to those in rhesus and AGM CD1D. On the basis of the findings, the evolutionary relationship of the CD1d molecules among the various primates and their HIV-1/SIV susceptibility will be discussed.
authors.affiliation: Laboratory of Virus Control, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Saito
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: W
authors.initials: W
authors.lastname: Akahata
authors.forename: E
authors.initials: E
authors.lastname: Ido
authors.forename: C
authors.initials: C
authors.lastname: Hidaka
authors.forename: K
authors.initials: K
authors.lastname: Ibuki
authors.forename: T
authors.initials: T
authors.lastname: Miura
authors.forename: M
authors.initials: M
authors.lastname: Hayami
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018949:Antigens, CD1 / Q000235:genetics*; D055610:Antigens, CD1d; D001483:Base Sequence; D002522:Chlorocebus aethiops / Q000235:genetics*; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology*; D019143:Evolution, Molecular; D006801:Humans; D008253:Macaca mulatta / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D018949:Antigens, CD1; D055610:Antigens, CD1d; C526877:CD1D protein, human; D004247:DNA