Stochastically modeling Listeria monocytogenes growth in farm tank milk.
This article presents a Listeria monocytogenes growth model in milk at the farm bulk tank stage. The main objective was to judge the feasibility and value to risk assessors of introducing a complex model, including a complete thermal model, within a microbial quantitative risk assessment scheme. Predictive microbiology models are used under varying temperature conditions to predict bacterial growth. Input distributions are estimated based on data in the literature, when it is available. If not, reasonable assumptions are made for the considered context. Previously published results based on a Bayesian analysis of growth parameters are used. A Monte Carlo simulation that forecasts bacterial growth is the focus of this study. Three scenarios that take account of the variability and uncertainty of growth parameters are compared. The effect of a sophisticated thermal model taking account of continuous variations in milk temperature was tested by comparison with a simplified model where milk temperature was considered as constant. Limited multiplication of bacteria within the farm bulk tank was modeled. The two principal factors influencing bacterial growth were found to be tank thermostat regulation and bacterial population growth parameters. The dilution phenomenon due to the introduction of new milk was the main factor affecting the final bacterial concentration. The results show that a model that assumes constant environmental conditions at an average temperature should be acceptable for this process. This work may constitute a first step toward exposure assessment for L. monocytogenes in milk. In addition, this partly conceptual work provides guidelines for other risk assessments where continuous variation of a parameter needs to be taken into account.
Albert
Isabelle
I
Met@risk Unit, Food Risk Methodologies, INRA, National Institute for Agricultural Research, France. albert@inapg.inra.fr
Pouillot
Régis
R
Denis
Jean-Baptiste
JB
D000818:Animals; D015169:Colony Count, Microbial; D005511:Food Handling; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D008892:Milk / Q000009:adverse effects* / Q000382:microbiology*; D008954:Models, Biological; D015233:Models, Statistical; D009010:Monte Carlo Method; D018570:Risk Assessment; D013269:Stochastic Processes; D013696:Temperature; D013997:Time Factors