title: Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast.
abstract: In contrast to animal and fungal cells, green plant cells contain one or multiple chloroplasts, the organelle(s) in which photosynthetic reactions take place. Chloroplasts are believed to have originated from an endosymbiotic event and contain DNA that codes for some of their proteins. Most chloroplast proteins are encoded by the nuclear genome and imported with the help of sorting signals that are intrinsic parts of the polypeptides. Here, we show that a chloroplast-located protein in higher plants takes an alternative route through the secretory pathway, and becomes N-glycosylated before entering the chloroplast.
authors.affiliation: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, S-90187 Umeå, Sweden.
authors.forename: Arsenio
authors.initials: A
authors.lastname: Villarejo
authors.forename: Stefan
authors.initials: S
authors.lastname: Burén
authors.forename: Susanne
authors.initials: S
authors.lastname: Larsson
authors.forename: Annabelle
authors.initials: A
authors.lastname: Déjardin
authors.forename: Magnus
authors.initials: M
authors.lastname: Monné
authors.forename: Charlotta
authors.initials: C
authors.lastname: Rudhe
authors.forename: Jan
authors.initials: J
authors.lastname: Karlsson
authors.forename: Stefan
authors.initials: S
authors.lastname: Jansson
authors.forename: Patrice
authors.initials: P
authors.lastname: Lerouge
authors.forename: Norbert
authors.initials: N
authors.lastname: Rolland
authors.forename: Gunnar
authors.initials: G
authors.lastname: von Heijne
authors.forename: Markus
authors.initials: M
authors.lastname: Grebe
authors.forename: Laszlo
authors.initials: L
authors.lastname: Bako
authors.forename: Göran
authors.initials: G
authors.lastname: Samuelsson
mesh_terms: D017360:Arabidopsis / Q000378:metabolism / Q000648:ultrastructure*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000235:genetics; D002736:Chloroplasts / Q000378:metabolism*; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D006031:Glycosylation; D049452:Green Fluorescent Proteins / Q000235:genetics; D008856:Microscopy, Fluorescence; D021382:Protein Sorting Signals; D021381:Protein Transport; D014162:Transfection
chemical_list: D029681:Arabidopsis Proteins; D021382:Protein Sorting Signals; D049452:Green Fluorescent Proteins; D002256:Carbonic Anhydrases