[Metabolic flux analysis of L-Tryptophan biosynthesis].
The metabolic flux balance model of L-Try synthesis by Corynebacterium glutamicum was constructed in this paper. Using this model, the metabolic flux distribution during the middle and late period were determined and the optimal flux distribution were calculated by linear program of MATLAB software. The analysis results indicate that 24.85% metabolic flux entered the HMP pathway and 75.15% entered the EMP cycle. But comparing to the optimal flux distributions, the production of L-Try should be improved from the genetic manipulation and fermentation control through reducing byproduct of amino acid and decreasing the metabolic flux of TCA and EMP.
Wang
Jian
J
College of Food Science and Bioengineering, Tianjin University of Science and Technology, Tianjin 300222, China.
Chen
Ning
N
Zhang
Bei
B
Tan
Qingqiao
Q
Zhang
Kexu
K
D053898:Biosynthetic Pathways; D048230:Corynebacterium glutamicum / Q000378:metabolism*; D005285:Fermentation; D008954:Models, Biological; D014364:Tryptophan / Q000096:biosynthesis*
D014364:Tryptophan