Synthesis of enantiopure (5R)-hydroxyhexane-2-one with immobilised whole cells of Lactobacillus kefiri.
Whole-cell reduction of (2,5)-hexanedione to yield highly enantiopure (5R)-hydroxyhexane-2-one (enantiomeric excess >99%) with Lactobacillus kefiri DSM 20587 was investigated. Cell immobilisation with sodium cellulose sulphate was chosen as the most suitable encapsulation matrix, giving an immobilisation yield of 40%. Despite the lowered biocatalytic activity from cell immobilisation, the bioreduction process was vastly improved with the help of reaction engineering techniques (batch to a plug flow reactor set-up). High selectivity (95%) and space-time yield (87 g L(-1) day(-1)) were achieved in the plug flow reactor. The biocatalyst remained active (68% residual activity) after 6 days of operation.
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Institute of Biotechnology, Forschungszentrum Juelich GmbH, Germany.
Fischbach
M
M
Huebner
H
H
Buchholz
R
R
Hummel
W
W
Daussmann
T
T
Wandrey
C
C
Liese
A
A
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D018914:Cells, Immobilized; D002482:Cellulose / Q000031:analogs & derivatives; D000441:Hexanols / Q000378:metabolism*; D007778:Lactobacillus / Q000166:cytology / Q000378:metabolism*
D000441:Hexanols; D002482:Cellulose