title: Yeast model systems for examining nitrogen oxide biochemistry/signaling.
abstract: The yeast Saccharomyces cerevisiae is an ideal model system for examining fundamental nitrogen oxide biochemistry. The utility of this model system lies in both the similarities and the differences between yeast and mammalian cells. The similarities between the two systems, with regards to many of the fundamental biochemical processes, allow studies in yeast to be extrapolated to mammalian systems. On the other hand, yeast has distinct differences that allow, for example, the facile examination of O2, pH, and genetic dependencies on a number of nitrogen oxide-mediated processes. Thus, the yeast system is amenable to experimentation that is otherwise problematic or impossible in mammalian systems. Herein, we present several examples of the utility of the yeast model system for studying the intimate details of basic nitrogen oxide biochemistry.
authors.affiliation: Department of Pharmacology, School of Medicine, Center for Health Sciences, University of California, Los Angeles, CA 90095-1772, USA.
authors.forename: Masaru
authors.initials: M
authors.lastname: Shinyashiki
authors.forename: Brenda E
authors.initials: BE
authors.lastname: Lopez
authors.forename: Chester E
authors.initials: CE
authors.lastname: Rodriguez
authors.forename: Jon M
authors.initials: JM
authors.lastname: Fukuto
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000378:metabolism; D008954:Models, Biological; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D010269:Paraquat / Q000633:toxicity; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D000975:Antioxidants; C060256:CUP2 protein, S cerevisiae; D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D009569:Nitric Oxide; D001616:beta-Galactosidase; D010269:Paraquat