title: [Expression of lysophosphatidic acid receptor in human ovarian cancer cell lines 3AO, SKOV3, OVCAR3 and its significance].
abstract: OBJECTIVE
  To investigate the expressions of lysophosphatidic acid (LPA) receptors at both mRNA and protein levels and their biological significance in different human ovarian cancer cell lines.
  
  
  METHODS
  Reverse transcription-polymerase chain reaction (RT-PCR), streptovidin peroxidase-conjugated (SP) immunohistochemical assay and Western blotting were employed to measure the expression levels of LPA(1), LPA(2) and LPA(3) mRNA and LPA(2) and LPA(3) protein in cultured human ovarian cancer cell lines 3AO.SKOV3, and OVCAR3.
  
  
  RESULTS
  All the ovarian cancer cell lines expressed LPA(1), LPA(2) and LPA(3) mRNAs and LPA(2) and LPA(3) proteins.
  
  
  CONCLUSIONS
  LPA(1), LPA(2) and LPA(3) expressions occur extensively in human epithelial ovarian cancer cell lines, and LPA(2) and LPA(3) may be involved in the development and progression of human ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, China. wangshjzh@163.com
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Wu
authors.forename: Wen-xue
authors.initials: WX
authors.lastname: Chen
authors.forename: Bao-en
authors.initials: BE
authors.lastname: Shan
authors.forename: Qing
authors.initials: Q
authors.lastname: Guo
mesh_terms: D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008246:Lysophospholipids / Q000096:biosynthesis* / Q000235:genetics; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D049368:Receptors, Lysophosphatidic Acid / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D008246:Lysophospholipids; D012333:RNA, Messenger; D049368:Receptors, Lysophosphatidic Acid; C032881:lysophosphatidic acid