title: G-tail telomere HPA: simple measurement of human single-stranded telomeric overhangs.
abstract: Accurate measurement of telomeric 3'-overhang (G-tail) lengths is essential for investigation of the biological effects of telomere dysfunction. G-tail telomere hybridization protection assay (Gt-telomere HPA) has the advantages of being simple to perform, accurate and highly sensitive for G tails as short as 20 nucleotides. Furthermore, Gt-telomere HPA is specific and quantitative for human G tails, and can be used to assay cell lysates as well as genomic DNA.
authors.affiliation: Department of Cellular and Molecular Biology, Division of Integrated Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima City, Hiroshima 734-8551, Japan. toshi@hiroshima-u.ac.jp
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Tahara
authors.forename: Mayuko
authors.initials: M
authors.lastname: Kusunoki
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yamanaka
authors.forename: Shusaku
authors.initials: S
authors.lastname: Matsumura
authors.forename: Toshinori
authors.initials: T
authors.lastname: Ide
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012680:Sensitivity and Specificity; D016615:Telomere / Q000737:chemistry / Q000235:genetics*
chemical_list: D004277:DNA, Single-Stranded