title: Molecular cloning and expression of human keratinocyte proline-rich protein (hKPRP), an epidermal marker isolated from calcium-induced differentiating keratinocytes.
abstract: We isolated a human gene encoding keratinocyte proline-rich protein (hKPRP). hKPRP gene is located in the region of epidermal differentiation complex on chromosome 1q21, and its approximately 2.5 kb mRNA encodes 579 amino acid protein with high proline content (18%). The mRNA level of hKPRP was markedly increased at both 7 and 14 d after treatment with 1.2 mM calcium in cultured normal human epidermal keratinocytes. In situ hybridization demonstrated that hKPRP was expressed in upper granular layer of normal epidermis with characteristic intermittent pattern. In psoriatic lesion, hKPRP expression was increased as compared with normal skin and showed continuous pattern. Immunohistochemical analysis also confirmed the expression of hKPRP at the protein level. Western blot analysis showed that hKPRP protein of approximately 70 kDa size was significantly increased by calcium in a time-dependent manner. In mouse tissue blot assays, the expression of KPRP was detected in stomach and skin tissues, and began at 17.5 embryonic days. Additionally, hKPRP expression was detected in the periderm of human fetal skin from 16 wk estimated gestational age. Together, these results suggest that hKPRP is an epidermal marker expressed in stratified squamous epithelia and has a potential role in keratinocytes differentiation.
authors.affiliation: Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: Woong-Hee
authors.initials: WH
authors.lastname: Lee
authors.forename: Sunhyae
authors.initials: S
authors.lastname: Jang
authors.forename: Jung-Suk
authors.initials: JS
authors.lastname: Lee
authors.forename: Young
authors.initials: Y
authors.lastname: Lee
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Seo
authors.forename: Kwan-Hee
authors.initials: KH
authors.lastname: You
authors.forename: Seung-Chul
authors.initials: SC
authors.lastname: Lee
authors.forename: Kwang-Il
authors.initials: KI
authors.lastname: Nam
authors.forename: Jin-Man
authors.initials: JM
authors.lastname: Kim
authors.forename: Sun-Ho
authors.initials: SH
authors.lastname: Kee
authors.forename: Jun-Mo
authors.initials: JM
authors.lastname: Yang
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Seo
authors.forename: Jang-Kyu
authors.initials: JK
authors.lastname: Park
authors.forename: Chang Deok
authors.initials: CD
authors.lastname: Kim
authors.forename: Jeung-Hoon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002118:Calcium / Q000494:pharmacology; D002454:Cell Differentiation; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D003001:Cloning, Molecular; D004622:Embryo, Mammalian / Q000378:metabolism; D000078404:Epidermal Cells; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D011565:Psoriasis / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism
chemical_list: D015415:Biomarkers; C476447:KPRP protein, human; D011506:Proteins; D012333:RNA, Messenger; D002118:Calcium