title: Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids.
abstract: A new method of electrospray-assisted laser desorption/ionization (ELDI) mass spectrometry, which combines laser desorption with post-ionization by electrospray, was applied to rapid analysis of solid materials under ambient conditions. Analytes were desorbed from solid metallic and insulating substrata using a pulsed nitrogen laser. Post-ionization produced high-quality mass spectra characteristic of electrospray, including protein multiple charging. For the first time, mass spectra of intact proteins were obtained using laser desorption without adding a matrix. Bovine cytochrome c and an illicit drug containing methaqualone were chosen in this study to demonstrate the applicability of ELDI to the analysis of proteins and synthetic organic compounds.
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 80424 Taiwan. jetea@mail.nsysu.edu.tw
authors.forename: Jentaie
authors.initials: J
authors.lastname: Shiea
authors.forename: Min-Zon
authors.initials: MZ
authors.lastname: Huang
authors.forename: Hsiu-Jung
authors.initials: HJ
authors.lastname: Hsu
authors.forename: Chi-Yang
authors.initials: CY
authors.lastname: Lee
authors.forename: Cheng-Hui
authors.initials: CH
authors.lastname: Yuan
authors.forename: Iwona
authors.initials: I
authors.lastname: Beech
authors.forename: Jan
authors.initials: J
authors.lastname: Sunner