SOCS3 promotes apoptosis of mammary differentiated cells.
Growth and function of the mammary gland is regulated by cytokines and modulated by suppressor of cytokine signalling (SOCS) proteins. In vitro experiments demonstrated that SOCS3 can inhibit PRL induction of milk protein gene expression and STAT5 activation. We explored the SOCS3 expression pattern during mouse mammary development and its regulation by PRL and GH in wild-type and STAT5a-null mammary tissue. Our results suggest that, in vivo, PRL stimulates SOCS3 expression in stromal adipocytes, independently of STAT5a stimulation. In mammary epithelial cells, SOCS3 expression appears to be related to STAT3 activation. Together, our results are consistent with a role of SOCS3 in the mammary gland by promoting apoptosis of differentiated cells (adipocytes during gestation and epithelial cells during involution).
Le Provost
Fabienne
F
Laboratory of Genetics and Physiology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA. Fabienne.LeProvost@jouy.inra.fr
Miyoshi
Keiko
K
Vilotte
Jean-Luc
JL
Bierie
Brian
B
Robinson
Gertraud W
GW
Hennighausen
Lothar
L
D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002454:Cell Differentiation; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D013006:Growth Hormone / Q000502:physiology*; D007774:Lactation / Q000502:physiology; D008321:Mammary Glands, Animal / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D011388:Prolactin / Q000502:physiology*; D050799:STAT5 Transcription Factor / Q000378:metabolism; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000502:physiology*
D050799:STAT5 Transcription Factor; C491411:Socs3 protein, mouse; C494093:Stat5a protein, mouse; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D011388:Prolactin; D013006:Growth Hormone