title: Effect of p47phox gene deletion on ROS production and oxygen sensing in mouse carotid body chemoreceptor cells.
abstract: Membrane potential in oxygen-sensitive type I cells in carotid body is controlled by diverse sets of voltage-dependent and -independent K(+) channels. Coupling of Po(2) to the open-closed state of channels may involve production of reactive oxygen species (ROS) by NADPH oxidase. One hypothesis suggests that ROS are produced in proportion to the prevailing Po(2) and a subset of K(+) channels closes as ROS levels decrease. We evaluated ROS levels in normal and p47(phox) gene-deleted [NADPH oxidase knockout (KO)] type I cells using the ROS-sensitive dye dihydroethidium (DHE). In normal cells, hypoxia elicited an increase in ROS, which was blocked by the specific NADPH oxidase inhibitor 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF, 3 mM). KO type I cells did not respond to hypoxia, but the mitochondrial uncoupler azide (5 microM) elicited increased fluorescence in both normal and KO cells. Hypoxia had no effect on ROS production in sensory and sympathetic neurons. Methodological control experiments showed that stimulation of neutrophils with a cocktail containing the chemotactic peptide N-formyl-Met-Leu-Phe (1 microM), arachidonic acid (10 microM), and cytochalasin B (5 microg/ml) elicited a rapid increase in DHE fluorescence. This response was blocked by the NADPH oxidase inhibitor diphenyleneiodonium (10 microM). KO neutrophils did not respond; however, azide (5 microM) elicited a rapid increase in fluorescence. Physiological studies in type I cells demonstrated that hypoxia evoked an enhanced depression of K+ current and increased intracellular Ca2+ levels in KO vs. normal cells. Moreover, AEBSF potentiated hypoxia-induced increases in intracellular Ca2+ and enhanced the depression of K+ current in low O(2). Our findings suggest that local compartmental increases in oxidase activity and ROS production inhibit the activity of type I cells by facilitating K+ channel activity in hypoxia.
authors.affiliation: Dept. of Physiology, University of Utah School of Medicine, 410 Chipeta Way, Salt Lake City, 84108 UT, USA.
authors.forename: L
authors.initials: L
authors.lastname: He
authors.forename: B
authors.initials: B
authors.lastname: Dinger
authors.forename: K
authors.initials: K
authors.lastname: Sanders
authors.forename: J
authors.initials: J
authors.lastname: Hoidal
authors.forename: A
authors.initials: A
authors.lastname: Obeso
authors.forename: L
authors.initials: L
authors.lastname: Stensaas
authors.forename: S
authors.initials: S
authors.lastname: Fidone
authors.forename: C
authors.initials: C
authors.lastname: Gonzalez
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002344:Carotid Body / Q000166:cytology / Q000502:physiology*; D015687:Cell Hypoxia / Q000502:physiology; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D017353:Gene Deletion; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008928:Mitochondria / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000502:physiology; D009861:Onium Compounds / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D018408:Patch-Clamp Techniques / Q000379:methods; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D024642:Potassium Channels, Voltage-Gated / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D019810:Sodium Azide / Q000494:pharmacology; D013450:Sulfones / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D009861:Onium Compounds; D010750:Phosphoproteins; D024642:Potassium Channels, Voltage-Gated; D017382:Reactive Oxygen Species; D013450:Sulfones; D016718:Arachidonic Acid; C002010:4-(2-aminoethyl)benzenesulfonylfluoride; D009240:N-Formylmethionine Leucyl-Phenylalanine; C007517:diphenyleneiodonium; D019810:Sodium Azide; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D011188:Potassium; D010100:Oxygen; D002118:Calcium