Isolation and inhibitory activity against ERK phosphorylation of hydroxyanthraquinones from rhubarb.
Five hydroxyanthraquinone compounds were purified from Chinese medicinal herb rhubarb by microwave-assisted extraction and silica gel column isolation. Their structures were identified by spectroscopic analysis. One of them, emodin, was found to block the ERK phosphorylation of PC3 cells at 20 microM. And two other compounds had potent proliferation inhibitory activity on A431 and PC3 cells at 20 microM. Furthermore, crude extracts revealed an antifungal activity stronger than that of purified compounds against several plant pathogens tested. Together, our study suggests that these compounds are biologically active and may lead to development of anticancer medicine.
Zhou
Xia
X
Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang 550025, PR China.
Song
Baoan
B
Jin
Linhong
L
Hu
Deyu
D
Diao
Chunling
C
Xu
Guangfang
G
Zou
Zhihui
Z
Yang
Song
S
D000818:Animals; D000880:Anthraquinones / Q000302:isolation & purification* / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002850:Chromatography, Gel / Q000379:methods; D004365:Drugs, Chinese Herbal; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D051379:Mice; D008872:Microwaves; D041681:NIH 3T3 Cells; D010766:Phosphorylation; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D012250:Rheum / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods
D000880:Anthraquinones; D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D048049:Extracellular Signal-Regulated MAP Kinases