title: Expressed protein ligation to study protein interactions: semi-synthesis of the G-protein alpha subunit.
abstract: Interactions between G proteins and GPCRs are fundamental for transmitting signals for a multitude of physiologic responses. Little is known regarding the protein-protein interface between the G protein and the receptor, much less the mechanisms for receptor activation of G proteins. Here, we will describe how expressed protein ligation will aid in the study of protein-protein interactions between semi-synthetic G alpha subunits and GPCRs.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Washington University, St. Louis, MO 63110, USA.
authors.forename: Lori L
authors.initials: LL
authors.lastname: Anderson
authors.forename: Garland R
authors.initials: GR
authors.lastname: Marshall
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Baranski
mesh_terms: D000818:Animals; D044385:GTP-Binding Protein alpha Subunits / Q000138:chemical synthesis*; D019204:GTP-Binding Proteins / Q000737:chemistry; D006801:Humans; D047668:Inteins; D008956:Models, Chemical; D015202:Protein Engineering / Q000379:methods; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*
chemical_list: D044385:GTP-Binding Protein alpha Subunits; D011506:Proteins; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins