[Mechanisms of p16 gene inactivation salivary adenoid cystic carcinoma].
OBJECTIVE
To study the mechanism of p16 gene inactivation in salivary adenoid cystic carcinoma.


METHODS
53 cases of freshly excised salivary adenoid cystic carcinomas were studied. Polymerase chain reaction (PCR) and single-stranded conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP) were used to detect deletion and mutation of p16 gene in salivary adenoid cystic carcinomas. Methylation specific PCR (MSP) was used to detect the methylation status of p16 gene.


RESULTS
The homozygous deletion, mutation and hypermethylation of p16 gene were noted in 16 cases (30.2%), 4 cases (7.5%) and 26 cases (49.1%) respectively in 53 cases of salivary adenoid cystic carcinomas.


CONCLUSIONS
The main inactivation mechanisms of p16 gene in salivary adenoid cystic carcinoma were hypermethylation and homozygous deletion. The mutation p16 gene was rare in salivary adenoid cystic carcinoma.
Guo
Xiao-ling
XL
Dept. of Oral and Maxillofacial Surgery, Hospital of Stomatology, Shandong University, Jinan 250012, China.
Sun
Shan-zhen
SZ
Wei
Feng-cai
FC
D003528:Carcinoma, Adenoid Cystic; D019175:DNA Methylation; D020868:Gene Silencing; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction