Slower skeletal muscle phenotypes are critical for constitutive expression of Hsp70 in overloaded rat plantaris muscle.
Heat shock protein 72 (Hsp70) is constitutively expressed in rat hindlimb muscles, reportedly in proportion to their content of type I myosin heavy chain. This distribution pattern has been suggested to result from the higher recruitment and activity of such muscles and/or a specific relationship between myosin phenotype and Hsp70 content. To differentiate between these possibilities, the fiber-specific distribution of Hsp70 was examined in male Sprague-Dawley rat plantaris under control conditions, following a fast-to-slow phenotypic shift in response to surgically induced overload (O) and in response to O when the phenotypic shift was prevented by 3,5,3'-triiodo-dl-thyronine administration. Constitutive expression of Hsp70 was restricted to type I and IIa fibers in plantaris from control rats, and this fiber-specific pattern of expression was maintained following O of up to 28 days, although Hsp70 content in the O muscle doubled. When O (for 40 days) of the plantaris was combined with 3,5,3'-triiodo-dl-thyronine administration, despite typical hypertrophy in the overloaded plantaris, prevention of the normal phenotypic transformation also blocked the increased expression of Hsp70 observed in euthyroid controls. Collectively, these data suggest that chronic changes in constitutive expression of Hsp70 with altered contractile activity appear critically dependent on fast-to-slow phenotypic remodeling.
O'Neill
David E T
DE
Thames Hall Rm. 2160C, School of Kinesiology, The Univ. of Western Ontario, London, ON, Canada N6A 3K7.
Aubrey
F Kris
FK
Zeldin
David A
DA
Michel
Robin N
RN
Noble
Earl G
EG
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D019417:Hindlimb Suspension / Q000502:physiology*; D006358:Hot Temperature; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D009119:Muscle Contraction; D018656:Muscle Fibers, Fast-Twitch / Q000737:chemistry / Q000502:physiology; D018657:Muscle Fibers, Slow-Twitch / Q000737:chemistry / Q000502:physiology; D018482:Muscle, Skeletal / Q000737:chemistry / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000032:analysis / Q000235:genetics; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D013961:Thyroid Gland / Q000502:physiology; D013997:Time Factors; D014284:Triiodothyronine / Q000494:pharmacology
D018840:HSP70 Heat-Shock Proteins; D014284:Triiodothyronine; D018995:Myosin Heavy Chains