Green tea with a high catechin content suppresses inflammatory cytokine expression in the galactosamine-injured rat liver.
Galactosamine is known to induce hepatic injury in rats and the galactosamine-induced hepatitis is believed to be similar to viral hepatitis both morphologically and functionally. In the present study, we examined how drinking green tea affects the gene expression of inflammatory cytokines which may be up-regulated in galactosamine-induced hepatitis. As has been reported, galactosamine caused hepatic injury in rats as evidenced by an increase in serum transaminase activities and histological observations of the liver. The results of the reverse transcription and polymerase chain reaction indicated an increased gene expression of inflammatory cytokines, tumor necrosis factor-alpha and interleukin-1beta, in the injured liver and the enzyme linked immunoassay showed an increase in the serum levels of these cytokines. Oral administration of green tea rich in catechins (Healthya green tea) restored these biomarkers in the galacotsamine-treated rats to near the control levels. These results suggest that the drinking of green tea with a high catechin content may help to prevent and/or attenuate the development of a certain type of hepatitis.
Abe
Kouichi
K
Department of Cellular Biochemistry, Graduate School of Nutritional and Environmental Sciences, and COE for the 21"s Century, University of Shizuoka, Shizuoka 422-8526, Japan.
Ijiri
Mitsuhiro
M
Suzuki
Takuji
T
Taguchi
Kyoko
K
Koyama
Yu
Y
Isemura
Mamoru
M
D000818:Animals; D002392:Catechin / Q000008:administration & dosage / Q000378:metabolism*; D056486:Chemical and Drug Induced Liver Injury; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005688:Galactosamine / Q000494:pharmacology / Q000633:toxicity*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008297:Male; D010936:Plant Extracts / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea / Q000737:chemistry*
D016207:Cytokines; D010936:Plant Extracts; D012333:RNA, Messenger; D013662:Tea; D005688:Galactosamine; D002392:Catechin