title: [Studies on the properties of Cecropin-XJ expressed in yeast from Xinjiang silkworm].
abstract: The purpose of this study is to investigate the properties of recombinant Cecropin-XJ isolated from Xinjing silkworm and expressed in Pichia yeast. According to Agarose Diffusion Assay, this recombinant Cecropin-XJ has exhibited an extreme heat-stable characteristic and the ability to kill ampicillin-resistant S. aureus and S. enterica. Moreover, we have observed that the Cecropin-XJ well tolerant to extreme acidic, basic, and high salt environments as well as resistant to 24 hours digestion by artificial gastric juice. The inhibition capability to S. aureus with 1mg Cecropin-XJ is equal to 1200U ampicillin. With a broad spectrum of antibacterial activities, the Cecropin-XJ is able to inhibit the Gram-positive bacteria and Gram-negative bacteria. These findings could lead it to a broad applications for agriculture, medical, domestic animal and food industry. The further investigation of the antibacterial mechanism of cecropin-XJ is needed.
authors.affiliation: Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. lzy1168@163.com
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Fuchun
authors.initials: F
authors.lastname: Zhang
authors.forename: Lun
authors.initials: L
authors.lastname: Cai
authors.forename: Gan
authors.initials: G
authors.lastname: Zhao
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D012831:Bombyx / Q000737:chemistry* / Q000378:metabolism; D054807:Cecropins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D015870:Gene Expression; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D010843:Pichia / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D054807:Cecropins; D019476:Insect Proteins