Vitamin E supplementation alters HDL-cholesterol concentration and paraoxonase activity in rabbits fed high-cholesterol diet: comparison with probucol.
Vitamin E and probucol are well-known antioxidants that prevent cells from the oxidative stress, which is a risk factor of atherosclerosis. Male rabbits were fed either 0.03% vitamin E or 0.05% probucol in a 0.5% high-cholesterol (HC) diet for 8 weeks. Vitamin E and probucol significantly suppressed an increase in plasma total-cholesterol (total-C) and low-density lipoprotein cholesterol compared to HC-control group. However, plasma high-density lipoprotein-cholesterol (HDL-C) and HDL-C/total-C ratio levels and plasma paraoxonase activity were only significantly higher in vitamin E group after 8 weeks. Hepatic ACAT activity was significantly lower in both vitamin E and probucol groups than in HC-control group, while HMG-CoA reductase activity was the highest only in the probucol group. Total fecal sterol content was significantly higher in probucol and vitamin E groups than in the two control groups. Some atherogenic signs were discovered in the aortic fatty streak of HC-control group, yet not in other groups. Hepatic mRNA expressions of apo B-100 and apo C-III were significantly lower in probucol group than in other groups. Vitamin E supplementation was found to alter the plasma HDL-C-related factors; meanwhile, probucol supplementation was very effective in enhancing cholesterol metabolism, except for a negative effect that reduced plasma HDL-C concentration.
Jeon
Seon-Min
SM
Institute of Genetic Engineering, Kyungpook National University, 1370 Sankyuk Dong Puk-ku, Daegu, 702-701, Korea.
Park
Yong Bok
YB
Kwon
Oh-Shin
OS
Huh
Tae-Lin
TL
Lee
Won-Ha
WH
Do
Kyung-Min
KM
Park
Taesun
T
Choi
Myung-Sook
MS
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000924:Anticholesteremic Agents / Q000008:administration & dosage*; D001011:Aorta / Q000187:drug effects; D043303:Aryldialkylphosphatase / Q000097:blood*; D001219:Aspartate Aminotransferases / Q000097:blood; D001483:Base Sequence; D001835:Body Weight / Q000187:drug effects; D008076:Cholesterol, HDL / Q000097:blood*; D017931:DNA Primers; D015870:Gene Expression; D008099:Liver / Q000187:drug effects; D008297:Male; D011341:Probucol / Q000008:administration & dosage*; D011817:Rabbits; D014810:Vitamin E / Q000008:administration & dosage*
D000924:Anticholesteremic Agents; D008076:Cholesterol, HDL; D017931:DNA Primers; D014810:Vitamin E; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D043303:Aryldialkylphosphatase; D011341:Probucol