title: T cell chemotaxis and chemokine release after Staphylococcus aureus interaction with polarized airway epithelium.
abstract: In response to bacterial infection, airway epithelium releases inflammatory mediators including cytokines and chemokines that lead to immune cell efflux and could stimulate the adaptive T cell immune response. The aim of our study was to analyze, in a double chamber culture, the chemokine changes in response to Staphylococcus aureus and their consequences for T cells. Our data show that S. aureus stimulates basolateral and apical release of IL-8 and eotaxin by airway epithelial cells. We also observed increased chemokine receptor expression on CD8+ and CD4+ T cells and enhanced chemotaxis of CD4+ T cells toward apical supernatant. Our data strongly suggest that S. aureus interaction with airway epithelium contributes to specific migration of T cells to inflamed sites.
authors.affiliation: Laboratoire d'Immuno-Pharmacologie Cellulaire et Moléculaire, EA3796, Université de Reims Champagne Ardennes, IFR53, Reims, France.
authors.forename: Sandie
authors.initials: S
authors.lastname: Escotte
authors.forename: Denise
authors.initials: D
authors.lastname: Al Alam
authors.forename: Richard
authors.initials: R
authors.lastname: Le Naour
authors.forename: Edith
authors.initials: E
authors.lastname: Puchelle
authors.forename: Moncef
authors.initials: M
authors.lastname: Guenounou
authors.forename: Sophie C
authors.initials: SC
authors.lastname: Gangloff
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology; D016764:Cell Polarity; D002478:Cells, Cultured; D054413:Chemokine CCL11; D018925:Chemokines / Q000378:metabolism*; D019742:Chemokines, CC / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D019707:Receptors, Chemokine / Q000378:metabolism; D020545:Respiratory Mucosa / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology*; D014132:Trachea / Q000166:cytology
chemical_list: C515456:CCL11 protein, human; D054413:Chemokine CCL11; D018925:Chemokines; D019742:Chemokines, CC; D016209:Interleukin-8; D019707:Receptors, Chemokine