15,16-dihydrotanshinone I suppresses the activation of BV-2 cell, a murine microglia cell line, by lipopolysaccharide.
Microglial activation has been implicated in neurodegenerative diseases. Therefore, inhibition of inflammation mediated by microglia is a strategy in neurodegenerative disease therapy. In this study, we isolated cryptotanshinone and 15,16-dihydrotanshinone I from Salvia miltiorrhiza, a traditional Korean herb medicine, by bioactivity-guided fractionation based on inhibitory effect on nitric oxide in a lipopolysaccharide-stimulated BV-2 cells, a murine microglial cell line. 15,16-Dihydotanshinoe I suppressed the expression of not only inducible nitric oxide synthase but also of interleukin-1beta, tumor necrosis factor-alpha, and of TNF-alpha converting enzyme.
Lee
Pyeongjae
P
Department of Herbal Pharmacology, Graduate School of East-West Medical Science, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Hur
Jinyoung
J
Lee
Jongseok
J
Kim
Jeongmin
J
Jeong
Jueun
J
Kang
Insook
I
Kim
Sun Yeou
SY
Kim
Hocheol
H
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D024202:Electrophoretic Mobility Shift Assay; D005663:Furans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D010616:Phenanthrenes / Q000494:pharmacology*; D011809:Quinones; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005663:Furans; D008070:Lipopolysaccharides; D010616:Phenanthrenes; D011809:Quinones; D012333:RNA, Messenger; D009569:Nitric Oxide; C000713095:dihydrotanshinone I; D052247:Nitric Oxide Synthase Type II