title: Immunoproteomics of outer membrane proteins and extracellular proteins of Shigella flexneri 2a 2457T.
abstract: Shigella flexneri 2a is an important pathogen causing bacillary dysentery in humans. In order to investigate any potential vaccine candidate proteins present in outer membrane proteins (OMPs) and extracellular proteins of S. flexneri 2a 2457T, we use the proteome mapping and database analyzing techniques. A subproteome map and database of OMPs were established first. One hundred and nine of the total 126 marked spots were cut out and processed to MALDI-TOF-MS and PMF. Eighty-seven spots were identified and they represented 55 OMP entries. Furthermore, immunoproteomics analysis of OMPs and extracellular proteins were performed. Total of 34 immunoreactive spots were identified, in which 22 and 12 were from OMPs and extracellular proteins, respectively. Eight novel antigens were found and some of these antigens may be potential vaccine candidate proteins. These results are useful for future studying of pathogenicity, vaccine, and novel antibacterial drugs. Maps and tables of all identified proteins are available on the Internet at www.proteomics.com.cn.
authors.affiliation: Beijing Institute of Biotechnology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Ying
authors.forename: Hengliang
authors.initials: H
authors.lastname: Wang
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Li
authors.forename: Junjun
authors.initials: J
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Zhaoxing
authors.initials: Z
authors.lastname: Shi
authors.forename: Erling
authors.initials: E
authors.lastname: Feng
authors.forename: Xiankai
authors.initials: X
authors.lastname: Liu
authors.forename: Guofu
authors.initials: G
authors.lastname: Su
authors.forename: Kaihua
authors.initials: K
authors.lastname: Wei
authors.forename: Xuemin
authors.initials: X
authors.lastname: Zhang
authors.forename: Peitang
authors.initials: P
authors.lastname: Huang
authors.forename: Liuyu
authors.initials: L
authors.lastname: Huang
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000032:analysis*; D012763:Shigella flexneri / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D020543:Proteome