title: The role of leptin in the development of the cerebral cortex in mouse embryos.
abstract: Leptin is detected in the sera, and leptin receptors are expressed in the cerebrum of mouse embryos, suggesting that leptin plays a role in cerebral development. Compared with the wild type, leptin-deficient (ob/ob) mice had fewer cells at embryonic day (E) 16 and E18 and had fewer 5-bromo-2'-deoxyuridine(+) cells at E14 and E16 in the neuroepithelium. Intracerebroventricular leptin injection in E14 ob/ob embryos increased the number of neuroepithelium cells at E16. In cultured neurosphere cells, leptin treatment increased Hes1 mRNA expression and maintained neural progenitors. Astrocyte differentiation was induced by low-dose (0.1 microg/ml) but not high-dose (1 microg/ml) leptin. High-dose leptin decreased Id mRNA and increased Ngn1 mRNA in neurosphere cells. The neuropeptide Y mRNA level in the cortical plate was lower in ob/ob than the wild type at E16 and E18. These results suggest that leptin maintains neural progenitors and is related to glial and neuronal development in embryos.
authors.affiliation: Department of Developmental Biology, Faculty of Medicine, Shimane University, Izumo, Japan. jun@med.shimane-u.ac.jp
authors.forename: Jun
authors.initials: J
authors.lastname: Udagawa
authors.forename: Ryuju
authors.initials: R
authors.lastname: Hashimoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Hatta
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sotomaru
authors.forename: Kyoji
authors.initials: K
authors.lastname: Hioki
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kagohashi
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Nomura
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Minami
authors.forename: Hiroki
authors.initials: H
authors.lastname: Otani
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000033:anatomy & histology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D020738:Leptin / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology; D046569:Neuroepithelial Cells / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D009929:Organ Size; D012333:RNA, Messenger / Q000378:metabolism; D018874:Spheroids, Cellular / Q000166:cytology / Q000378:metabolism; D000072056:Transcription Factor HES-1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; D020738:Leptin; D009478:Neuropeptide Y; D012333:RNA, Messenger; D000072056:Transcription Factor HES-1; C489734:Hes1 protein, mouse