Cloning and expression of canine interferon-alpha genes in Escherichia coli.
We cloned five new subtypes of cDNA encoding canine interferon-alpha (CaIFN-alpha) from a canine epithelial cell line. CaIFN-alphas were divided into two groups by amino acid sequences and a molecular phylogenic tree. Two subtypes of them were expressed in Escherichia coli, and IFN proteins were purified. Recombinant CaIFN-alphas were highly species-specific and showed antiviral activity against Vesicular stomatitis New Jersey virus and canine adenovirus-1 , but not against canine herpesvirus-1.
Taira
Osamu
O
Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Watanugi
Itsuki
I
Hagiwara
Yuko
Y
Takahashi
Masaki
M
Arai
Setsuo
S
Sato
Hisaaki
H
Maehara
Nobutoshi
N
D006516:Adenoviruses, Canine; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D003588:Cytopathogenic Effect, Viral; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D004926:Escherichia coli; D015870:Gene Expression; D016898:Interferon-alpha / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity; D018116:Vesiculovirus
D017931:DNA Primers; D018076:DNA, Complementary; D016898:Interferon-alpha