Quercetin protects C6 glial cells from oxidative stress induced by tertiary-butylhydroperoxide.
The anti-oxidant and cyto-protective activity of quercetin against tertiary-butylhydroperoxide (t-BOOH) induced oxidative stress on C6 glial cells is reported. Exposure of the cells to t-BOOH resulted in a significant increase in cytotoxicity, reactive oxygen species (ROS) generation and lipid peroxidation. There was a significant increase in DNA strand breaks and fall in reduced GSH levels in cells exposed to t-BOOH. A significant increase in calcium ion influx was noticed in cells exposed to t-BOOH. Pre-treatment of cells with quercetin, vitamin C (vit C), Trolox, and deferoxamine (DFO) significantly inhibited t-BOOH induced cytotoxicity and ROS generation. Pretreatment of cells with quercetin, Trolox and DFO inhibited the DNA damage, maintained higher GSH levels and prevented calcium influx significantly. Although vit C protected the cells from cytotoxicity induced by t-BOOH, the intracellular Ca(2+) levels were significantly higher than the control cells. However, anti-oxidants like butylated hydroxy toluene (BHT), vitamin E (vit E), N-acetyl cysteine (NAC) did not have significant cytoprotection against t-BOOH induced oxidative injury in C6 glial cells.
Gitika
B
B
Defence Institute of Physiology & Allied Sciences, Timarpur, Lucknow Road, Delhi, 110054, India.
Sai Ram
M
M
Sharma
S K
SK
Ilavazhagan
G
G
Banerjee
P K
PK
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage; D004347:Drug Interactions; D015227:Lipid Peroxidation / Q000187:drug effects*; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress; D011794:Quercetin / Q000494:pharmacology*; D020122:tert-Butylhydroperoxide / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D020122:tert-Butylhydroperoxide; D011794:Quercetin