Antisense-mediated knockdown of iNOS expression in the presence of cytokines.
The impact of nitric oxide (NO) synthesized after activation by proinflammatory cytokines and/or bacterial products by an inducible NO synthase (iNOS) is still contradictory. Various methods to inhibit iNOS expression or activity have been established. A relatively new approach to inhibit iNOS-derived NO production is the antisense (AS) technique, which theoretically provides a specific and efficient method for inhibiting gene expression and function. This chapter focuses on the application of iNOS-specific AS-oligodeoxynucleotide (ODN) and highlights some of the pitfalls that must be considered to use this technique effectively.
Hemmrich
Karsten
K
Department of Plastic Surgery, Hand Surgery, Burn Center, University Hospital of the Aachen, University of Technology, Aachen, Germany.
Suschek
Christoph V
CV
Kolb-Bachofen
Victoria
V
D000818:Animals; D001483:Base Sequence; D016207:Cytokines / Q000494:pharmacology*; D017931:DNA Primers; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
D016207:Cytokines; D017931:DNA Primers; D009573:Nitrites; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D052247:Nitric Oxide Synthase Type II