title: Influence of two fertilization regimens on the amounts of organic acids and phenolic compounds of tronchuda cabbage (Brassica oleracea L. Var. costata DC).
abstract: A phytochemical study was undertaken on tronchuda cabbage (Brassica oleracea L. var. costata DC) cultivated under conventional and organic practices and collected at different times. Six organic acids (aconitic, citric, ascorbic, malic, shikimic, and fumaric acids) were identified and quantified by HPLC-UV. Qualitative and quantitative differences were noted between internal and external leaves. Analysis of the phenolics of the internal leaves was achieved by HPLC-DAD, and the phenolic profile obtained was revealed to be distinct from that of the external leaves. By this means were identified and quantified 11 compounds: 3-p-coumaroylquinic acid, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-(caffeoyl)sophoroside-7-O-glucoside, kaempferol 3-O-(sinapoyl)sophoroside-7-O-glucoside, kaempferol 3-O-(feruloyl)sophoroside-7-O-glucoside, kaempferol 3-O-sophoroside, two isomeric forms of 1,2-disinapoylgentiobiose, 1-sinapoyl-2-feruloylgentiobiose, 1,2,2'-trisinapoylgentiobiose, and 1,2'-disinapoyl-2-feruloylgentiobiose. In general, internal leaves exhibited more constant chemical profiles.
authors.affiliation: REQUIMTE/Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha 164, 4050-047 Porto, Portugal.
authors.forename: Carla
authors.initials: C
authors.lastname: Sousa
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.forename: Joana
authors.initials: J
authors.lastname: Rangel
authors.forename: Graciliana
authors.initials: G
authors.lastname: Lopes
authors.forename: José A
authors.initials: JA
authors.lastname: Pereira
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Seabra
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
mesh_terms: D000383:Agriculture / Q000379:methods; D001937:Brassica / Q000737:chemistry* / Q000254:growth & development*; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005308:Fertilizers; D010636:Phenols / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D012621:Seasons
chemical_list: D002264:Carboxylic Acids; D005308:Fertilizers; D010636:Phenols