title: Influence of secondary reactions on the synthetic efficiency of DHAP-aldolases.
abstract: The effect of secondary reactions on DHAP-dependent aldolase stereoselective synthesis yields is reported. The fuculose-1-phosphate aldolase catalyzed synthesis between DHAP and Cbz-S-alaninal has been chosen as case study. It has been demonstrated that DHAP is not only chemically degraded in the reaction medium, but also enzymatically. The last reaction has been shown to take place when type II aldolases are used as biocatalysts. In order to minimize the effect of non-desired reactions, temperature reduction has been shown to be favorable, and operation at 4 degrees C has been chosen as appropriate. On the other hand, the fed-batch addition of DHAP also increased the synthesis yields and, combined with low temperature, led to almost quantitative conversion.
authors.affiliation: Departament d'Enginyeria Química, Escola Tècnica Superior d'Enginyeria, Unitat de Biocatàlisi Aplicada associada al IIQA, CSIC, Universitat Autònoma de Barcelona, Edifici Cn, 08193-Bellaterra, Spain.
authors.forename: Trinitat
authors.initials: T
authors.lastname: Suau
authors.forename: Gregorio
authors.initials: G
authors.lastname: Alvaro
authors.forename: M Dolors
authors.initials: MD
authors.lastname: Benaiges
authors.forename: Josep
authors.initials: J
authors.lastname: López-Santín
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry; D000446:Aldehyde-Lyases / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D003080:Cold Temperature; D004099:Dihydroxyacetone Phosphate / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology*; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; C046025:alaninal; D004099:Dihydroxyacetone Phosphate; D000446:Aldehyde-Lyases; C036027:L-fuculosephosphate aldolase; D000409:Alanine