title: Antichagasic activity of komaroviquinone is due to generation of reactive oxygen species catalyzed by Trypanosoma cruzi old yellow enzyme.
abstract: A novel potent trypanocidal diterpene, komaroviquinone, was reduced by Trypanosoma cruzi old yellow enzyme (TcOYE) to its semiquinone radical. The reductase activity in trypanosome lysates was completely immunoabsorbed by anti-TcOYE antibody. Since TcOYE is expressed throughout the T. cruzi life cycle, komaroviquinone is an interesting candidate for developing new antichagasic drugs.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
authors.forename: Nahoko
authors.initials: N
authors.lastname: Uchiyama
authors.forename: Zakayi
authors.initials: Z
authors.lastname: Kabututu
authors.forename: Bruno K
authors.initials: BK
authors.lastname: Kubata
authors.forename: Fumiyuki
authors.initials: F
authors.lastname: Kiuchi
authors.forename: Michiho
authors.initials: M
authors.lastname: Ito
authors.forename: Junko
authors.initials: J
authors.lastname: Nakajima-Shimada
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
authors.forename: Kei
authors.initials: K
authors.lastname: Ohkubo
authors.forename: Shunichi
authors.initials: S
authors.lastname: Fukuzumi
authors.forename: Samuel K
authors.initials: SK
authors.lastname: Martin
authors.forename: Gisho
authors.initials: G
authors.lastname: Honda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Urade
mesh_terms: D000818:Animals; D002384:Catalysis; D014355:Chagas Disease / Q000188:drug therapy*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D011809:Quinones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014344:Trypanocidal Agents / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use*; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000235:genetics
chemical_list: D004224:Diterpenes; D011809:Quinones; D017382:Reactive Oxygen Species; D014344:Trypanocidal Agents; C508550:komaroviquinone; D009252:NADPH Dehydrogenase