title: [Preparation of chiral alcohol by stereoselective reduction of acetophenone and chloroacetophenone with yeast cells].
abstract: Four strains of microorganisms which have activity of chloroacetophenone reduction were screened, in which Saccharomyces cerevisiae B5 showed the highest activity and good stereoselectivity. This strains showed different activity on the reduction of various substrates in the following order: 2'-chloroacetophenone > 2-chloromethylacetophenone > 4'-chloroacetophenone > 3'-chloroacetophenone > acetophenone. Ethanol is the best cosubstrate and its optimal concentration is 5%.
authors.affiliation: Institute of Biochemical Engineering, College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China. oozzmm@163.com
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Ou
authors.forename: Jianping
authors.initials: J
authors.lastname: Wu
authors.forename: Lirong
authors.initials: L
authors.lastname: Yang
authors.forename: Peilin
authors.initials: P
authors.lastname: Cen
mesh_terms: D000098:Acetophenones / Q000737:chemistry* / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism; D007218:Industrial Microbiology; D007700:Kinetics; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D015003:Yeasts / Q000737:chemistry / Q000378:metabolism; D002721:omega-Chloroacetophenone / Q000737:chemistry / Q000378:metabolism
chemical_list: D000098:Acetophenones; D000438:Alcohols; D002721:omega-Chloroacetophenone