[Application of HMGN2-tag constructs to analysis of HMGN2 distribution in HeLa cells].
This study sought to clarify the distribution of HMGN2 in HeLa cells. The recombinant eukaryotic expression vectors pcDNA3. 1-myc-his-HMGN2 and pEGFP-N1-HMGN2 were constructed, and then were transfected into HeLa cells. immunocytochemistry staining indicated that HMGN2 were present not only in HeLa nucleus but also in the cytoplasm. The presence of HMGN2 was also detected in the culture supernatant by ELISA with rabbit anti-serum against HMGN2 and mouse anti-His6 monoclonal antibodies. The confocal microscope observation showed the same subcellular localization as that of immunocytochemistry staining. There results suggested that HMGN2 could be present in the nucleus and cytoplasm of HeLa cell as well as in the extracellular environment.
Xiong
Wenbi
W
Research Unit of Infection & Immunity, West China Medical Center, Sichuan University, Chengdu 610041, China.
Feng
Yun
Y
Huang
Ning
N
Wu
Qi
Q
Li
Xuan
X
Wang
Boyao
B
D000818:Animals; D000911:Antibodies, Monoclonal; D024242:HMGN2 Protein / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000494:pharmacology; D014162:Transfection
D000911:Antibodies, Monoclonal; D024242:HMGN2 Protein; D011993:Recombinant Fusion Proteins