title: Four pregnancies in nonmosaic Klinefelter's syndrome using cryopreserved-thawed testicular spermatozoa.
abstract: OBJECTIVE
  To investigate feasibility of using cryopreserved-thawed testicular spermatozoa from patients with nonmosaic Klinefelter's syndrome for intracytoplasmic sperm injection (ICSI).
  
  
  METHODS
  Case report.
  
  
  METHODS
  University-based hospital and IVF clinic.
  
  
  METHODS
  Six patients with nonmosaic Klinefelter's syndrome who underwent testicular sperm extraction for ICSI.
  
  
  METHODS
  Microdissection testicular sperm extraction (TESE) and ICSI.
  
  
  METHODS
  We compared results of ICSI using cryopreserved testicular spermatozoa with those previously reported in Klinefelter's syndrome and those in nonobstructive azoospermia patients using cryopreserved testicular spermatozoa at our institution with respect to embryo cleavage rate, implantation rate, and pregnancy outcome.
  
  
  RESULTS
  Four of six patient couples with successful microdissection TESE achieved pregnancy using cryopreserved-thawed testicular spermatozoa. One pregnancy resulted in early-pregnancy abortion, two in delivery of healthy singleton girls, and one delivery of a healthy singleton boy.
  
  
  CONCLUSIONS
  Cryopreserved-thawed testicular spermatozoa can be used successfully for ICSI in patients with nonmosaic Klinefelter's syndrome.
authors.affiliation: Department of Urology, Teikyo University School of Medicine, Tokyo, Japan. okada@med.teikyo-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Okada
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Goda
authors.forename: Satoru
authors.initials: S
authors.lastname: Muto
authors.forename: Osamu
authors.initials: O
authors.lastname: Maruyama
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Koshida
authors.forename: Shigeo
authors.initials: S
authors.lastname: Horie
mesh_terms: D000328:Adult; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007713:Klinefelter Syndrome / Q000235:genetics* / Q000473:pathology*; D008297:Male; D011247:Pregnancy; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology; D013737:Testis / Q000166:cytology* / Q000502:physiology