Thiopental sodium reduces glutamate extracellular levels in rat intact prefrontal cortex.
To investigate the effect of thiopental sodium on glutamate extracellular levels in the prefrontal cortex (PFC) of rats, a microdialysis probe was inserted into the PFC, the perfusate was collected every 10 min throughout the experiment with thiopental sodium ip or perfused into the PFC locally. The concentrations of glutamate in the perfusate were determined by reversed-phase high performance liquid chromatography. Thiopental sodium 30 mg kg(-1) ip significantly decreased glutamate levels in the perfusate after 10, 20, 30, and 40 min; glutamate levels in the perfusate were also decreased from 10 to 90 min after thiopental sodium 50 mg kg(-1) ip. Thiopental sodium with concentrations of 30, 100, or 300 microM perfused into the PFC also decreased glutamate levels in the perfusate significantly. The results suggest that thiopental sodium decreases glutamate extracellular levels in rat intact PFC.
Liu
Hongliang
H
Department of Anesthesiology, Zhongda Hospital, Southeast University, 210000, Nanjing, Jiangsu Province, People's Republic of China. liuhl75@163.com
Yao
Shanglong
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003627:Data Interpretation, Statistical; D005110:Extracellular Space / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D006993:Hypnotics and Sedatives / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008297:Male; D017551:Microdialysis; D004856:Postural Balance / Q000187:drug effects; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012018:Reflex / Q000187:drug effects; D013874:Thiopental / Q000494:pharmacology*
D006993:Hypnotics and Sedatives; D018698:Glutamic Acid; D013874:Thiopental