Mimicking reverse protein splicing by three-segment tandem peptide ligation.
Here we report a bi-directional and interchangeable three-segment peptide ligation of N, M, and C-segments, mimicking the reverse process of protein splicing to form, in tandem, a tripartite NMC-peptide using a synthetic intein, a role served by the M-segment with an N-terminal Ser or Thr and a C-terminal thioester.
Tam
James P
JP
Department of Microbiology and Immunology, Vanderbilt University, MCN A5102A, Nashville, TN 37232, USA. james.tam@vanderbilt.edu
Eom
Khee Dong
KD
D000595:Amino Acid Sequence; D003545:Cysteine / Q000737:chemistry; D047668:Inteins; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods; D019154:Protein Splicing; D012694:Serine / Q000737:chemistry; D013912:Threonine / Q000737:chemistry
D010446:Peptide Fragments; D013912:Threonine; D012694:Serine; D003545:Cysteine