Capillary-channeled polymer (C-CP) fibers as a stationary phase in microbore high-performance liquid chromatography columns.
Microbore columns utilizing polypropylene capillary-channeled polymer (C-CP) fibers as the stationary phase in high-performance liquid chromatography (HPLC) have been investigated. The polypropylene C-CP fiber diameter is approximately 50 microm, with eight channels along the periphery of the fiber ranging in diameter from approximately 12 to 35 microm. The polypropylene C-CP fibers were packed into fluorinated ethylene propylene (FEP) tubing, 1.3 mm inner diameter, with lengths of 500, 750, and 1,000 mm, to examine the effects of increased column length with regards to plate height, resolution and analysis time. The low backpressures characteristic of the C-CP fiber stationary phases allow the length of the column to be increased without significantly decreasing the specific permeability. The high specific permeability (approximately 5x10(-8) cm2) of the C-CP packed microbore columns yields a relatively low backpressure of 2.35 MPa at the highest flow rate of 17 microL/s (54 mm/s) for a 1,000 mm column. Radial compression of the soft-walled FEP tubing is accomplished by pulling the 1.7 mm o.d. column through a 1.4 mm diameter orifice. Reducing the inner diameter of the column from 1.3 to 1.0 mm lowered the interstitial fraction from 47% to 42%, decreased the A-term contributions to band broadening, resulted in a significant decrease in average plate height (approximately 30%), and increased resolution (approximately 36%) at identical linear velocities. Although the lower void volume of the radially compressed column increased the backpressure from 0.57 to 2.11 MPa at a linear velocity of approximately 20 mm/s, the specific permeability only decreased from approximately 7x10(-8) to 4x10(-8) cm2.
Stanelle
Rayman D
RD
Department of Chemistry, Biosystems Research Complex, Clemson University, Clemson, SC 29634, USA.
Mignanelli
Massimiliano
M
Brown
Phil
P
Marcus
R Kenneth
RK
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D014498:Uracil / Q000737:chemistry
D011108:Polymers; D014498:Uracil