Analysis of a novel human gene, LOC92912, over-expressed in hypopharyngeal tumours.
We have identified by differential display a number of novel genes that are expressed in hypopharyngeal head and neck squamous cell carcinoma. We report here the characterisation of one of these novel human genes, LOC92912, that encodes a protein of 375 amino acids. The protein contains a RWD domain, a coiled-coil, and an E2 ubiquitin conjugating enzyme domain. LOC92912 is upregulated in about 85% of tumour samples. It is expressed in tumour masses and in invasive epithelium, and is located in the cytoplasm of cells. To gain insights into its functions, we identified potential interacting partners by immunoaffinity purification of the flag tagged protein followed by MALDI peptide mass fingerprinting mass spectrometry. Actin and six actin-binding proteins were unambiguously identified as potential interacting partners, suggesting that LOC92912's functions may be linked with the cytoskeleton. This novel human gene may represent a new target for cancer therapeutics.
Seghatoleslam
Atefeh
A
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, 1 Rue Laurent Fries, BP 10142, Illkirch Cedex, Strasbourg 67404, France.
Zambrano
Alberto
A
Millon
Regine
R
Ganguli
Gitali
G
Argentini
Manuela
M
Cromer
Anne
A
Abecassis
Joseph
J
Wasylyk
Bohdan
B
D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D019295:Computational Biology; D003593:Cytoplasm / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling; D052138:Genes, Neoplasm; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011485:Protein Binding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D044763:Ubiquitin-Conjugating Enzymes
D000199:Actins; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; C507338:UBE2Q2 protein, human; D044763:Ubiquitin-Conjugating Enzymes