Determination of anthocyanins from camu-camu (Myrciaria dubia) by HPLC-PDA, HPLC-MS, and NMR.
Camu-camu [Myrciaria dubia (HBK) McVaugh] is a small fruit native to the Amazonian rain forest. Its anthocyanin profile has now been investigated for the first time. Fruits from two different regions of the São Paulo state, Brazil, were analyzed. The major anthocyanins were isolated by high-speed countercurrent chromatography. HPLC-PDA, HPLC-MS/MS, and 1H NMR were used to confirm the identity of the main anthocyanins of camu-camu. Cyanidin-3-glucoside was identified as the major pigment in the fruits from both regions, representing 89.5% in the fruits produced in Iguape and 88.0% in those from Mirandópolis, followed by the delphinidin-3-glucoside, ranging between 4.2 and 5.1%, respectively. Higher total anthocyanin contents were detected in the fruits from Iguape (54.0 +/- 25.9 mg/100 g) compared to those from Mirandópolis (30.3 +/- 6.8 mg/100 g), most likely because of the lower temperatures in the Iguape region.
Zanatta
Cinthia Fernanda
CF
Department of Food Science, Faculty of Food Engineer, State University of Campinas, UNICAMP, Post Office Box 6121, Campinas, São Paulo, 13083-970, Brazil.
Cuevas
Elyana
E
Bobbio
Florinda O
FO
Winterhalter
Peter
P
Mercadante
Adriana Z
AZ
D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002980:Climate; D003377:Countercurrent Distribution; D005638:Fruit / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D027822:Myrtaceae / Q000737:chemistry*
D000872:Anthocyanins