title: Two-layer method in short-term pancreas preservation for successful islet isolation.
abstract: BACKGROUND
  We sought to determine whether the two-layer method (TLM) offers advantages over UW storage solution for locally procured pancreata with cold ischemia time of <8 hours for successful islet isolation.
  
  
  METHODS
  From October 2003 through February 2005, 22 human pancreata were procured locally from cadaveric donors and preserved using UW solution (n = 11) or TLM (n = 11).
  
  
  RESULTS
  Donor characteristics were similar in the two groups, with no statistical difference. Cold ischemia time was 4.5 +/- 0.6 (2.5 to 8) hours in the UW and 5.1 +/- 0.5 (3 to 8) hours in TLM group (P > .05). Organs preserved with TLM were exposed to PFC for 4 +/- 0.5 (2 to 7.5) hours. After TLM preservation, 8 of 11 (72%) pancreata yielded >300,000 IEQ pancreatic islets, which met all criteria for clinical transplantation; after UW cold storage, only 3 of 11 isolations were equally successful (27%) (P < .05). Mean IEQ was higher in the TLM than in the UW group: 349,000 +/- 37,000 vs 277,800 +/- 34,000; IEQ/g was also higher at 5100 +/- 760 vs 3000 +/- 570, respectively (P < .05). Islet quality, characterized by purity, viability, and insulin SI, did not differ statistically in the two groups: 67 +/- 4 vs 74 +/- 4%, 87 +/- 2 vs 83 +/- 4%, and 4 +/- 0.7 vs 4.8 +/- 1, respectively (P > .05).
  
  
  CONCLUSIONS
  The Two Layer Method for locally procured human pancreata with cold ischemia time lower than 8 hours offers significant advantage over UW cold storage increasing the pancreatic islet isolation yield and the isolation success rate.
authors.affiliation: Department of Surgery, Columbia University, 630 West 168th Street, P&S 17-514, New York, NY 10032, USA. pw2004@columbia.edu
authors.forename: P
authors.initials: P
authors.lastname: Witkowski
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: Q
authors.initials: Q
authors.lastname: Guo
authors.forename: E
authors.initials: E
authors.lastname: Poumian-Ruiz
authors.forename: S
authors.initials: S
authors.lastname: Cernea
authors.forename: K
authors.initials: K
authors.lastname: Herold
authors.forename: M A
authors.initials: MA
authors.lastname: Hardy
mesh_terms: D000241:Adenosine; D000293:Adolescent; D000328:Adult; D000493:Allopurinol; D005978:Glutathione; D006801:Humans; D007328:Insulin; D007515:Islets of Langerhans / Q000166:cytology*; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010179:Pancreas; D011887:Raffinose; D020858:Tissue and Organ Harvesting / Q000379:methods*
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose