Autoantibodies against HSP70 family proteins were detected in the cerebrospinal fluid from patients with multiple sclerosis.
We evaluated the specific IgG antibodies against heat shock proteins (HSPs) in cerebrospinal fluids (CSF) from patients with multiple sclerosis (MS). ELISA was employed to examine IgG antibodies against ten HSPs (HSP27, alphaA and alphaB crystallins, HSP60, CCT, Mycobacterium bovis HSP65, Escherichia coli GroEL, HSP70, HSC70 and HSP90) in CSF from 30 patients with MS, and 25 patients with motor neuron diseases (MND). Significantly higher antibody titers against HSP70 and HSC70 proteins were found in CSF obtained from patients with MS as compared with MND independent of CSF total protein, IgG concentrations and IgG indices, respectively. The antibody titers against HSP70 were indicated to be significantly higher in the progressive cases than in cases of remission. The results suggest that IgG antibodies against specific types of HSPs especially HSP70 family proteins (HSP70 and HSC70) in CSF may play an important role in the pathophysiology of MS through the modification of immune response and cytoprotective functions of molecular chaperons.
Chiba
Susumu
S
Department of Neurology, Sapporo Medical University School of Medicine, Minami 1-Jo Nishi 16 chome, Chuo-ku, Sapporo 060-8543, Japan. chiba@sapmed.ac.jp
Yokota
Shin-ichi
S
Yonekura
Kazumi
K
Tanaka
Shingo
S
Furuyama
Hiroyasu
H
Kubota
Hiroshi
H
Fujii
Nobuhiro
N
Matsumoto
Hiroyuki
H
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D050883:HSC70 Heat-Shock Proteins / Q000134:cerebrospinal fluid / Q000276:immunology*; D018840:HSP70 Heat-Shock Proteins / Q000134:cerebrospinal fluid / Q000276:immunology*; D006360:Heat-Shock Proteins / Q000134:cerebrospinal fluid / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D016472:Motor Neuron Disease / Q000134:cerebrospinal fluid*; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*
D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007074:Immunoglobulin G