Electron spin resonance assay of ascorbyl radical generation in mouse hippocampal slices during and after kainate-induced seizures.
As an index of oxidative status, we analyzed ascorbyl radical generation during and after kainate-induced seizures in mouse hippocampus, using an ESR spectrometer equipped with a special tissue-type quartz cell. A specific doublet ESR spectrum was observed after seizures, and the g value and the hyperfine coupling constant (hfcc) of the spectrum were identical with those of ascorbyl radical itself. Antiepileptic zonisamide inhibited the generation of ascorbyl radical accompanying the seizures.
Masumizu
Toshiki
T
JEOL, Ltd., Tokyo 196-8558 and Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan. masumizu@ph.sojo-u.ac.jp
Noda
Yasuko
Y
Mori
Akitane
A
Packer
Lester
L
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000032:analysis* / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D004830:Epilepsy, Tonic-Clonic / Q000139:chemically induced / Q000378:metabolism; D018690:Excitatory Amino Acid Agonists; D005609:Free Radicals / Q000032:analysis* / Q000378:metabolism*; D006624:Hippocampus / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D007555:Isoxazoles / Q000494:pharmacology; D007608:Kainic Acid; D008297:Male; D017895:Manganese Compounds / Q000737:chemistry; D051379:Mice; D010087:Oxides / Q000737:chemistry; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D000078305:Zonisamide
D000927:Anticonvulsants; D018690:Excitatory Amino Acid Agonists; D005609:Free Radicals; D007555:Isoxazoles; D017895:Manganese Compounds; D010087:Oxides; D000078305:Zonisamide; C027424:manganese oxide; D001205:Ascorbic Acid; D007608:Kainic Acid