Therapeutic nanoreactors: combining chemistry and biology in a novel triblock copolymer drug delivery system.
Triblock copolymeric nanoreactors are introduced as an alternative for liposomes as encapsulating carrier for prodrug activating enzymes. Inosine-adenosine-guanosine preferring nucleoside hydrolase of Trypanosoma vivax, a potential prodrug activating enzyme, was encapsulated in nanometer-sized vesicles constructed of poly(2-methyloxazoline)-block-poly(dimethylsiloxane)-block-(2-methyloxazoline) triblock copolymers. The nanoreactor is functionalized by incorporation of bacterial porins, OmpF or Tsx, in the reactor wall. Efficient cleavage of three natural substrates and one prodrug, 2-fluoroadenosine, by the nanoreactors was demonstrated.
Ranquin
An
A
Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel (VUB) and Vlaams Interuniversitair Instituut voor Biotechnology (VIB6), Pleinlaan 2, 1050 Brussels, Belgium. aranquin@vub.ac.be
Versées
Wim
W
Meier
Wolfgang
W
Steyaert
Jan
J
Van Gelder
Patrick
P
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage; D019149:Bioreactors; D016503:Drug Delivery Systems; D029968:Escherichia coli Proteins / Q000008:administration & dosage; D066298:In Vitro Techniques; D007700:Kinetics; D009699:N-Glycosyl Hydrolases / Q000008:administration & dosage / Q000378:metabolism; D049329:Nanostructures; D036103:Nanotechnology; D010539:Permeability; D011108:Polymers; D018272:Porins / Q000008:administration & dosage; D011355:Prodrugs / Q000008:administration & dosage; D011991:Receptors, Virus / Q000008:administration & dosage; D016689:Trypanosoma vivax / Q000201:enzymology
D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C109237:OmpF protein; D011108:Polymers; D018272:Porins; D011355:Prodrugs; D011991:Receptors, Virus; C066743:Tsx protein, E coli; D009699:N-Glycosyl Hydrolases; C110539:inosine-adenosine-guanosine-preferring nucleoside hydrolase