title: [Establishment of beta block matching technique].
abstract: The purpose of this study was to establish beta block matching technique. DNA was extracted from whole blood by salting-out method, beta block matching was performed by PCR and GeneScan technique. The results showed that the length of fragments amplificated in 100 samples was different and the range of them was 91-197 bp. Amplification fragments could be divided into four regions: 91-93, 105-113, 125-139 and 177-197 bp respectively. 91 bp DNA fragments could be found in all of samples. The numbers of DNA fragments with different length have been shown high polymorphism and they focused on the range of seven to twenty four. In conclusion, the beta block matching technique is reliable and applicable to the selection of hematopoietic stem cell transplantation donors.
authors.affiliation: Blood Center of Zhejiang Province, Hangzhou 310006, China.
authors.forename: Fa-Ming
authors.initials: FM
authors.lastname: Zhu
authors.forename: Qin-Feng
authors.initials: QF
authors.lastname: Lü
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Hai-Qin
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Qi-Hua
authors.initials: QH
authors.lastname: Fu
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D004247:DNA / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D015236:HLA-C Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D018380:Hematopoietic Stem Cell Transplantation; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D015235:HLA-B Antigens; D015236:HLA-C Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D004247:DNA