First isolation of Japanese encephalitis from Culex quinquefasciatus in Thailand.
Isolation of Japanese encephalitis (JE) virus using C6/36 cell and immunofluorescence virus antigen detection techniques was attempted from female mosquitoes collected with CDC gravid traps in Samut Songkhram Province in the central region and in Phuket Province in southern Thailand, in 2003. One thousand and eighty female mosquitoes including 6 species of the Culicidae family (Culex quinquefasciatus, Cx. gelidus, Cx. tritaeniorhynchus, Cx. whitmorei, Cx. vishnui complex, Cx. s.g. culiciomyia) (pooled by specific specimen), were processed for virus isolation. Two pools of Cx. quinquefasciatus yielded a JE virus isolation. This represents the first report of JE virus isolation from Cx. quinquefasciatus in Thailand.
Nitatpattana
Narong
N
Center for Vaccine Development, Research Center for Emerging Viral Diseases, Institute of Science and Technology for Research and Development, Mahidol University, Nakhon Pathom, Thailand.
Apiwathnasorn
Chamnarn
C
Barbazan
Philippe
P
Leemingsawat
Somjai
S
Yoksan
Sutee
S
Gonzalez
Jean-Paul
JP
D000818:Animals; D003465:Culex / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D007303:Insect Vectors / Q000821:virology*; D013785:Thailand