title: Chemical sympathectomy attenuates myenteric but not dorsal vagal complex Fos-like immunoreactivity induced by cholecystokinin-8 in the rat.
abstract: Vagotomy and capsaicin treatment attenuate dorsal vagal complex (DVC) but not myenteric Fos-like immunoreactivity (Fos-LI) induced by cholecystokinin-8 (CCK-8). The goal of this experiment is to test the role of the sympathetic nervous system in the pathway by which CCK-8 increases myenteric Fos-LI. Adult male Sprague-Dawley rats were pretreated with guanethidine sulfate (40 mg/kg daily for 5 weeks) or vehicle intraperitoneally (IP), and injected with CCK-8 (40 microg/kg) or saline IP. Fos-LI was then quantified in the DVC and the myenteric neurons of the duodenum and jejunum using a diaminobenzidine reaction. Guanethidine pretreatment attenuated myenteric but not DVC Fos-LI induced by CCK-8. These findings demonstrate that sympathetic neurons play a role in mediating the myenteric Fos-LI response to CCK. They also suggest differential mediation of myenteric and DVC responses to CCK.
authors.affiliation: Gastroenterology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL 36088, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Gulley
authors.forename: Shannon J
authors.initials: SJ
authors.lastname: Raboin
authors.forename: Sheryce C
authors.initials: SC
authors.lastname: Henley
authors.forename: Wai-Chu
authors.initials: WC
authors.lastname: Chan
authors.forename: Sanjay K
authors.initials: SK
authors.lastname: Sharma
authors.forename: Leonard H
authors.initials: LH
authors.lastname: Billups
authors.forename: Alton R
authors.initials: AR
authors.lastname: Esdaile
authors.forename: Cherese N
authors.initials: CN
authors.lastname: Sullivan
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Moran
authors.forename: Ayman I
authors.initials: AI
authors.lastname: Sayegh
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002766:Cholecystokinin / Q000502:physiology*; D006145:Guanethidine / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D009197:Myenteric Plexus / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013563:Sympathectomy, Chemical; D017779:Sympathetic Fibers, Postganglionic / Q000187:drug effects / Q000378:metabolism*; D013565:Sympatholytics / Q000494:pharmacology; D014628:Vagotomy; D014630:Vagus Nerve / Q000187:drug effects / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D016760:Proto-Oncogene Proteins c-fos; D013565:Sympatholytics; C410885:cholecystokinin 8; D002766:Cholecystokinin; D006145:Guanethidine