Nociceptive response and adenine nucleotide hydrolysis in synaptosomes isolated from spinal cord of hypothyroid rats.
Purinergic system exerts a significant influence on the modulation of pain pathways at the spinal site. Adenosine has antinociceptive properties in experimental and clinical situations, while ATP exerts pronociceptive actions in different pain models. In this study we investigated the hydrolysis of ATP to adenosine in synaptosomes from spinal cord in parallel with the nociceptive response of rats at different ages after hypothyroidism induction. Hypothyroidism elicited a significant increase in AMP hydrolysis to adenosine in synaptosomes from spinal cord of rats subjected to neonatal hypothyroidism and in 420-day-old rats submitted to thyroidectomy. Accordingly, these rats presented an analgesic response as a consequence of hypothyroidism. In contrast, the ATP hydrolysis was decreased in the spinal cord of 60-day-old hypothyroid rats in parallel with a significant increase in nociceptive response. These results indicate the involvement of adenine nucleotides in the control of the hypothyroidism-induced nociceptive response during development.
Bruno
Alessandra Nejar
AN
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Avenida Ramiro Barcellos, Porto Alegre, RS, Brazil.
Pochmann
Daniela
D
Ricachenevsky
Felipe Klein
FK
Fontella
Fernanda Urruth
FU
Bonan
Carla Denise
CD
Dalmaz
Carla
C
Barreto-Chaves
Maria Luiza M
ML
Sarkis
João José Freitas
JJ
D000227:Adenine Nucleotides / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D007037:Hypothyroidism / Q000378:metabolism*; D008297:Male; D010146:Pain / Q000378:metabolism*; D010147:Pain Measurement; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000166:cytology*; D013574:Synaptosomes / Q000378:metabolism*
D000227:Adenine Nucleotides