[Comparison of Galla Chinesis and sodium fluoride on growth and metabolism of bacteria in biofilm model].
OBJECTIVE
To compare the antibacterial effect between sodium fluoride and Galla Chinesis in bioflim model. To evaluate the feasibility of application of Gala Chinesis in the clinical practice of caries prevention.


METHODS
Streptococcus mutans, Streptococcus sanguis, Streptococcus salivarius and Actinomyces naeslundii had been chosen as the experimental bacteria. In the experiment, biofilmn model was constructed, and two agents were added in the media. After a period of continuous culture, the number of bacteria adhering on the HA disc was examed, the planktonic pH in the flow cell was recorded continuously, and the morphology of the biofilmn formed on the HA disc was observed by SEM.


RESULTS
(1) Galla Chinesis could inhibit the growth of the four oral bacteria in the biofilm just as sodium fluoride. (2) Galla Chinesis and sodium fluoride could prevent the descent of the planktonic pH in the flow cell, but Galla Chinesis was less efficient than sodium fluoride. (3) The biofilms formed after application of Galla Chinesis and fluoride, but the bacterial cells had less matrix than those applicated with sucrose.


CONCLUSIONS
Galla Chinesis is one kind of effective cariostatic natural agents.
Zhu
Bing
B
Key. Laboratory of Oral Biomedical Engineering Ministry of Education, Sichuan University, Chengdu 610041, China.
Li
Ji-yao
JY
Huang
Zheng-wei
ZW
Li
Hong
H
Zhou
Xue-dong
XD
D000190:Actinomyces; D001419:Bacteria; D018441:Biofilms; D002327:Cariostatic Agents; D003731:Dental Caries; D005459:Fluorides; D010710:Phosphates; D012969:Sodium Fluoride; D013295:Streptococcus mutans; D013298:Streptococcus sanguis; D013395:Sucrose
D002327:Cariostatic Agents; D010710:Phosphates; C012980:fluorophosphate; D013395:Sucrose; D012969:Sodium Fluoride; D005459:Fluorides