Quantitative DNA perturbations of p53 in endometriosis: analysis of American and Icelandic cases.
OBJECTIVE
To investigate quantitative aberrations involving p53 copy numbers in eutopic endometrial and endometriotic tissue from two populations.


METHODS
Comparative analysis of normal and diseased tissue.


METHODS
Tissue specimens collected in Iceland and USA.


METHODS
Subjects with moderate/severe endometriosis (Iceland, n = 26; USA, n = 45). Paraffin-embedded tissue from 19 matched Icelandic cases and seven unaffected controls. American cases were fresh surgical tissue from 17 matched cases and 28 unaffected controls. DNA isolation and real-time polymerase chain reaction (PCR) with TaqMan assay were performed.


METHODS
The frequency of p53 loss and/or gain based on quantitative differences for copy numbers of p53 located on chromosome (17p) and GAPDH on a control locus (chromosome 12p).


RESULTS
Among American cases, significant p53 gain (n = 13) or loss (n = 4) was observed in 17 of 21 cases. In Icelandic cases this was not seen to the same degree. Mean normalized p53 values were 3.46 and 1.16 copies per reaction, respectively. Significant differences were observed between normalized p53 in the control blood and affected tissue for the American and Icelandic cases compared to standard GAPDH control but not in normal Icelandic and American endometrium.


CONCLUSIONS
The results continue to support a role for nonrandom somatic p53 locus alterations in the pathogenesis of late or severe-stage endometriosis. Differences between Icelandic and American subjects have implications for generalization of genome-wide approaches.
Gylfason
Jon Torfi
JT
Medical Faculty, University of Iceland, Reykjavik, Iceland.
Dang
Dianne
D
Petursdottir
Vigdis
V
Benediktsdottir
Kristrun R
KR
Geirsson
Reynir T
RT
Poindexter
Alfred
A
Mitchell-Leef
Dorothy
D
Buster
John E
JE
Carson
Sandra A
SA
Simpson
Joe Leigh
JL
Bischoff
Farideh Z
FZ
D004247:DNA / Q000235:genetics*; D004715:Endometriosis / Q000235:genetics*; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007055:Iceland; D040641:Quantitative Trait Loci / Q000235:genetics*; D014481:United States
D004247:DNA