title: [Effect of goblet cell in rat intestine on the restitution process of the gut barrier after hemorrhagic shock].
abstract: OBJECTIVE
  To investigate the changes of the goblet cells in the intestine during the restitution process of the gut barrier after hemorrhagic shock.
  
  
  METHODS
  Forty-nine Sprague-Dawley rats with body weight of 250-300 g were divided into control group (n=7) and experimental group (n=42). Rats in the experimental group was further divided into 6 groups (n=7 each) according to different time point at 1, 3, 6, 12, 24, and 36 hours after hemorrhagic shock resuscitation. The specimens from ileum tissue were taken to observe the morphological chan ges of the intestinal mucosa. The number of goblet cells was determined by light microscope and/or electron microscope. The contents of trefoil factor family 3 (TFF3) of goblet cells were examined using GC-9A gas chromatographic instrument.
  
  
  RESULTS
  After hemorrhagic shock, mucosal epithelial injury was obvious in the small intestine. Tissue restitution was found after 3 hours, and mostly established after 12 hours. Following tissue restitution,the denuded mucosal surface was covered intensively by goblet cells. The number of goblet cells on the intestinal mucosa was reduced significantly from 243+/- 13 at 1 h to 157+/- 9 at 24 h (r=- 0.910, P< 0.01), and returned to normal level at 36 h. In the experimental group, the content of TFF3 in the intestinal mucosa increased significantly at 12 hours, decreased, but was still higher at 24 hours (t=3.24, P< 0.05).
  
  
  CONCLUSIONS
  The goblet cells play a key role in the restitution of intestinal mucosa. High expression of TFF3 may facilitate the intestinal mucosal restitution in the early phase.
authors.affiliation: The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Jian-xing
authors.initials: JX
authors.lastname: Chang
authors.forename: Shuang
authors.initials: S
authors.lastname: Chen
authors.forename: Long-yuan
authors.initials: LY
authors.lastname: Jiang
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Ma
authors.forename: Rui-ming
authors.initials: RM
authors.lastname: Chang
authors.forename: Zi-tong
authors.initials: ZT
authors.lastname: Huang
mesh_terms: D000818:Animals; D020397:Goblet Cells / Q000378:metabolism*; D007082:Ileum / Q000166:cytology; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D009479:Neuropeptides / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000378:metabolism*; D000071165:Trefoil Factor-3
chemical_list: D009479:Neuropeptides; C099037:TFF3 protein, rat; D000071165:Trefoil Factor-3