Design and synthesis of cyclic and linear peptide-agarose tools for baiting interacting protein partners of GPCRs.
A ligation strategy for the synthesis of cyclic and linear peptides covalently linked to agarose beads designed as baits to identify new interacting partners of intracellular loops of the V2 vasopressin receptor, a member of the G-protein-coupled receptor family, is reported. The peptide-resin conjugates were subsequently shown to interact specifically with a fraction of proteins present in cellular lysates.
Granier
Sébastien
S
UMR 5203 CNRS, U 661 INSERM, Université Montpellier 1, Université Montpellier 2, Institut de Génomique Fonctionnelle, 141 rue de la Cardonille, 34094 Montpellier Cedex, France.
Jean-Alphonse
Frédéric
F
Déméné
Hélène
H
Guillon
Gilles
G
Pascal
Robert
R
Mendre
Christiane
C
D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism; D015195:Drug Design; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000378:metabolism*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry / Q000378:metabolism*; D017483:Receptors, Vasopressin / Q000737:chemistry / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry*
D010455:Peptides; D010456:Peptides, Cyclic; D011506:Proteins; D043562:Receptors, G-Protein-Coupled; D017483:Receptors, Vasopressin; D012685:Sepharose