Activated cyclic AMP-response element binding protein (CREB) is expressed in a myelin-associated protein in chick.
Cyclic AMP response element (CRE) is a specific DNA sequence, which mediates transcriptional activation in the response to the cyclic AMP-activated and protein kinase A dependent signaling pathway. In the present study, phosphorylated CRE binding protein (CREB) immunoreactivity was mainly localized in the white matter of chick central nervous system (CNS). We have further confirmed the specificity of phospho-CREB immunoreaction in myelin using demyelinated optic nerve induced by lysophophatidylcholine (LPC), which is known to produce demyelination with little axonal damage. Double immunofluorescent analyses with myelin basic protein (MBP) and transferrin binding protein (TfBP), oligodendrocyte marker showed that phospho-CREB recognized a myelin-related protein in chick. Immunoblot analyses showed that phospho-CREB recognized a protein with molecular weights of approximately 70 kDa. Our data suggest that the antigen recognized by phospho-CREB is a myelin-associated protein in the chick CNS.
Kim
Dong Woon
DW
Department of Anatomy, Seoul National University College of Medicine, Seoul, South Korea.
Chang
Jae Hyuk
JH
Park
Sang Wook
SW
Jeon
Gye Sun
GS
Seo
Je Hoon
JH
Cho
Sa Sun
SS
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002645:Chickens; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D003711:Demyelinating Diseases; D006801:Humans; D007150:Immunohistochemistry; D008244:Lysophosphatidylcholines / Q000378:metabolism; D004676:Myelin Basic Protein / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism*; D009900:Optic Nerve / Q000166:cytology / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D033863:Transferrin-Binding Proteins / Q000378:metabolism
D017362:Cyclic AMP Response Element-Binding Protein; D008244:Lysophosphatidylcholines; D004676:Myelin Basic Protein; D033863:Transferrin-Binding Proteins