Current developments in gene transfection agents.
DNA can be delivered into the cell nucleus either using physical means or specific carriers that carry the genes into the cells for gene expression). Various carriers for delivering genes have been investigated which can be divided into two main groups: viral carriers where the DNA to be delivered is inserted into a virus, and cationic molecular carriers that form electrostatic interactions with DNA). Successful gene therapy depends on the efficient delivery of genetic materials into the cells nucleus and its effective expression within these cells). Although at present the in vivo expression levels of synthetic molecular gene vectors are lower than for viral vectors and gene expression is transient, these vehicles are likely to present several advantages including safety, low-immunogenicity, capacity to deliver large genes and large-scale production at low-cost). The two leading classes of synthetic gene delivery systems that have been mostly investigated are cationic lipids and cationic polymers). This review discusses recent developments in viral vectors, physical means and molecular gene carriers). The last part focuses on our recent studies in developing a new series of biodegradable polycations for in vitro and in vivo gene transfection).
Azzam
Tony
T
Department of Medicinal Chemistry and Natural Products, School of Pharmacy-Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 91120, Israel.
Domb
Abraham J
AJ
D000818:Animals; D001692:Biological Transport; D002412:Cations; D004337:Drug Carriers; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008055:Lipids; D011108:Polymers; D015398:Signal Transduction; D014162:Transfection; D014780:Viruses / Q000235:genetics
D002412:Cations; D004337:Drug Carriers; D008055:Lipids; D011108:Polymers