An early intestinal mucosal source of gamma interferon is associated with resistance to and control of Cryptosporidium parvum infection in mice.
Resistance to and control of Cryptosporidium parvum infection in mice in the absence of adaptive immunity appears to be gamma interferon (IFN-gamma) dependent. Using an IFN-gamma-neutralizing antibody in a murine model, we demonstrated increased susceptibility to infection within 24 h. We correlated this early resistance and control with increased mucosal expression of IFN-gamma and demonstrate that CD8+ T-cell receptor alphabeta intestinal intraepithelial lymphocytes express and secrete this cytokine shortly after infection. The rapid kinetics of IFN-gamma expression and secretion by naive CD8+ T cells in response to a protozoan pathogen have not previously been demonstrated.
Leav
Brett A
BA
Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center, Box 041, 750 Washington Street, Boston, MA 02111, USA. bleav@tufts-nemc.org
Yoshida
Masaru
M
Rogers
Kathleen
K
Cohen
Seth
S
Godiwala
Nihal
N
Blumberg
Richard S
RS
Ward
Honorine
H
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000953:Antigens, Protozoan / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003457:Cryptosporidiosis / Q000276:immunology*; D016785:Cryptosporidium parvum; D004198:Disease Susceptibility; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D007421:Intestine, Small / Q000276:immunology / Q000473:pathology; D051379:Mice; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis
D000906:Antibodies; D000953:Antigens, Protozoan; D016693:Receptors, Antigen, T-Cell, alpha-beta; D007371:Interferon-gamma