The anti-anaphylactic effect of the gall of Rhus javanica is mediated through inhibition of histamine release and inflammatory cytokine secretion.
The immediate-type allergic reaction (anaphylaxis) is involved in many allergic diseases such as asthma, allergic rhinitis and sinusitis. We investigated the effect of the gall of Rhus javanica (GRJ) on the model of the immediate-type allergic reaction, and studied its possible mechanisms. GRJ inhibited compound 48/80-induced systemic reactions in mice. GRJ attenuated immunoglobulin (Ig) E-mediated local allergic reactions. In addition, GRJ dose dependently decreased histamine release from rat peritoneal mast cells activated by compound 48/80 or IgE. The decreasing effect of GRJ on the histamine release was mediated by the modulation of cAMP and [Ca2+]i in mast cells. Furthermore, GRJ decreased the phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated TNF-alpha and IL-6 secretion in human mast cells. The inhibitory effect of GRJ on the pro-inflammatory cytokine was c-Jun N-terminal kinase and nuclear factor-kappaB dependent. Our findings provide evidence that GRJ inhibits mast cell-derived immediate-type allergic reactions, and suggest the possible mechanisms of action.
Kim
Sang-Hyun
SH
Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, 700-422, South Korea.
Park
Hyo-Hyun
HH
Lee
Soyoung
S
Jun
Chang-Duk
CD
Choi
Byung-Ju
BJ
Kim
Sang-Yong
SY
Kim
Sug-Hyun
SH
Kim
Dae-Keun
DK
Park
Jeong-Suk
JS
Chae
Byeong-Suk
BS
Shin
Tae-Yong
TY
D000707:Anaphylaxis / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005707:Gallic Acid / Q000302:isolation & purification / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010529:Peritoneal Cavity / Q000166:cytology; D018515:Plant Leaves; D051381:Rats; D029045:Rhus / Q000737:chemistry*; D013997:Time Factors; D003189:p-Methoxy-N-methylphenethylamine
D018926:Anti-Allergic Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D003189:p-Methoxy-N-methylphenethylamine; D005707:Gallic Acid; D000242:Cyclic AMP; D002118:Calcium