[Expression of apoptosis-related proteins in bone marrow neutrophils of patients with paroxysmal nocturnal hemoglobinuria].
This study was aimed to evaluate expression levels of CD166, Fas and apoptosis-related proteins in bone marrow neutrophils of PNH patients and normal controls, and to analyze their correlation in order to explore whether exist apoptosis abnormality in BM neutrophils of PNH patients. The expression levels of CD16b, Fas and Bax, Bcl-2 in BM neutrophils of PNH patients and normal controls were assayed by flow cytometry; the difference of expression levels between patients and controls, and expression correlation between CD16b and apoptosis-related proteins were compared. The results showed that (1) the expression levels of CD16b on BM neutrophils of patients and controls were (20.36 +/- 9.05)% and (71.34 +/- 26.8)% respectively (P = 0.01); (2) the expression levels of CD95 on BM neutrophils of patients and controls were (62.83 +/- 32.11)% and (48.00 +/- 38.52)% respectively, there were no significant difference between CD95 expressions in BM neutrophils of PNH patients and controls and no significant correlation between expression of CD95 and CD16b on BM neutrophils of PNH patients (P > 0.05); (3) the expression levels of Bcl-2 in BM neutrophil cytoplasma of patients and controls were (8.64 +/- 5.40)% and (16.82 +/- 15.39)% respectively, there were no significant difference between Bcl-2 expression of patients and controls, and no significant correlation between the expression of Bcl-2 and CD16b in BM neutrophil cytoplasma of PNH patients (P > 0.05); (4) the expression levels of Bax in BM neutrophil cytoplasma of patients and control were (30.47 +/- 22.15)% and (48.47 +/- 15.99)% respectively, there were no significant difference between the Bax expressions of patients and controls, and no significant correlation between the Bax and CD16b expressions in BM neutrophil cytoplasma of PNH patients. In conclusion, BM neutrophils of PNH patients expressed apoptosis-related CD95, Bcl-2 and Bax without significant difference from the normal controls, and without significant correlation with the CD16b expression. It is suggested that the cell growth and decrease of PNH patients possibly are independent of abnormal apoptosis.
Cao
Yan-Ran
YR
Institute of Hematology, Hematological Hospital of Union Medical University of China, The Chinese Academy of Medical Sciences, Tianjin 300020, China.
Shao
Zong-Hong
ZH
Liu
Hong
H
Zhao
Ming-Feng
MF
He
Guang-Sheng
GS
Shi
Jun
J
Bai
Jie
J
Fu
Rong
R
Tu
Mei-Feng
MF
Wang
Hua-Quan
HQ
Xing
Li-Min
LM
Cui
Zheng-Zhu
ZZ
Sun
Juan
J
Jia
Hai-Rong
HR
Yang
Tian-Ying
TY
D000293:Adolescent; D000328:Adult; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis*; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D006457:Hemoglobinuria, Paroxysmal / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D017452:Receptors, IgG / Q000096:biosynthesis; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis; D019014:fas Receptor / Q000096:biosynthesis
D051017:Apoptosis Regulatory Proteins; C428200:FCGR3B protein, human; D058851:GPI-Linked Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D017452:Receptors, IgG; D051028:bcl-2-Associated X Protein; D019014:fas Receptor