Murine mesenchymal and embryonic stem cells express a similar Hox gene profile.
Using degenerate oligonucleotide primers targeting the homeobox domain, we amplified by PCR and sequenced 723 clones from five murine cell populations and lines derived from embryonic mesoderm and adult bone marrow. Transcripts from all four vertebrate Hox clusters were expressed by the different populations. Hierarchical clustering of the data revealed that mesenchymal stem cells (MSCs) and the embryonic stem (ES) cell line D3 shared a similar Hox expression profile. These populations exclusively expressed Hoxb2, Hoxb5, Hoxb7, and Hoxc4, transcripts regulating self-renewal and differentiation of other stem cells. Additionally, Hoxa7 transcript quantified by real-time PCR strongly correlated (r2=0.89) with the number of Hoxa7 clones identified by sequencing, validating that data from the PCR screen reflects differences in Hox mRNA abundance between populations. This is the first study to catalogue Hox transcripts in murine MSCs and by comparative analyses identify specific Hox genes that may contribute to their stem cell character.
Phinney
Donald G
DG
Center for Gene Therapy, Tulane University Health Science Center, New Orleans, LA 70112, USA. dphinne@tulane.edu
Gray
Andrew J
AJ
Hill
Katy
K
Pandey
Amitabh
A
D000818:Animals; D001483:Base Sequence; D004622:Embryo, Mammalian / Q000166:cytology; D020869:Gene Expression Profiling; D005801:Genes, Homeobox / Q000502:physiology*; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D013234:Stem Cells / Q000378:metabolism*
D018398:Homeodomain Proteins; C492047:Hoxa7 protein, mouse; D009363:Neoplasm Proteins