New weakly expressed cell cycle-regulated genes in yeast.
We present an approach combining bioinformatics prediction with experimental microarray validation to identify new cell cycle-regulated genes in Saccharomyces cerevisiae. We identify in the order of 100 new cell cycle-regulated genes and show by independent data that these genes in general tend to be more weakly expressed than the genes identified hitherto. Among the genes not previously suggested to be periodically expressed we find genes linked to DNA repair, cell size monitoring and transcriptional control, as well as a number of genes of unknown function. Several of the gene products are believed to be phosphorylated by Cdc28. For many of these new genes, homologues exist in Schizosaccharomyces pombe and Homo sapiens for which the expression also varies with cell cycle progression.
de Lichtenberg
Ulrik
U
Center for Biological Sequence Analysis, BioCentrum, Technical University of Denmark, Building 208, DK-2800 Lyngby, Denmark.
Wernersson
Rasmus
R
Jensen
Thomas Skøt
TS
Nielsen
Henrik Bjørn
HB
Fausbøll
Anders
A
Schmidt
Peer
P
Hansen
Flemming Bryde
FB
Knudsen
Steen
S
Brunak
Søren
S
D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D018816:Genes, cdc; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D018797:Cell Cycle Proteins; D029701:Saccharomyces cerevisiae Proteins