title: Localization of Eutr2, a locus controlling susceptibility to DES-induced uterine inflammation and pyometritis, to RNO5 using a congenic rat strain.
abstract: In some rat strains chronic administration of exogenous estrogens induces pyometritis, an inflammation of the uterus associated with infection, suggesting that there is genetic variation in susceptibility to estrogen-induced inflammation and pyometritis. In this article we report that following 10 weeks of treatment with the synthetic estrogen diethylstilbestrol (DES), Fisher 344 (F344) rats exhibit modest uterine inflammation and a 0% incidence of pyometritis. By contrast, under identical experimental conditions, Brown Norway (BN) rats exhibit significant inflammation and a 100% incidence of pyometritis. Similarly, we also observed profound uterine inflammation and a 100% incidence of pyometritis in a congenic rat strain in which a segment of RNO5 from the BN strain is carried on the F344 strain. These data suggest that a locus on RNO5 controls both the magnitude of DES-induced uterine inflammation and susceptibility to DES-induced pyometritis. This locus, designated Eutr2, resides within the same segment of RNO5 as the Eutr1 locus, which confers susceptibility to E2-induced pyometritis in an F2 population generated in a cross between the BN and August x Copenhagen 9935, Irish (ACI) strains.
authors.affiliation: Department of Biological Sciences, Oakland University, Rochester, Michigan 48309, USA.
authors.forename: Jyotsna
authors.initials: J
authors.lastname: Pandey
authors.forename: Karen A
authors.initials: KA
authors.lastname: Gould
authors.forename: Rodney D
authors.initials: RD
authors.lastname: McComb
authors.forename: James D
authors.initials: JD
authors.lastname: Shull
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Wendell
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics*; D004054:Diethylstilbestrol / Q000633:toxicity*; D004716:Endometritis / Q000139:chemically induced* / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN
chemical_list: D004054:Diethylstilbestrol