title: Studies on microbiological degradation of tobacco tar.
abstract: A bacterium, strain MR4, was isolated from tobacco tar-contaminated soil and identified as Klebsiella oxytoca based on morphology, physiological and biochemical characteristics and phylogenetic analysis of 16S rDNA sequence. The organism grew optimally at 34 degrees C and pH 7.0 to 7.5. During growth on tobacco tar the isolate produced acid materials, which caused the drop of pH in the cultures. GC/MS analysis indicated that the isolate had the ability to degrade phenolic compounds, heterocyclic compounds, especially nicotine in tobacco tar. The degradation rate of strain MR4 was 75.56% for nicotine, 35.84% to 58.16% for hydroxybenzenes and the other aromatic compounds, 29.15% to 65.56% for heterocyclics, and 35.17% to 82.59% for hydrocarbons.
authors.affiliation: Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, P. R. China.
authors.forename: Aidong
authors.initials: A
authors.lastname: Ruan
authors.forename: Hang
authors.initials: H
authors.lastname: Min
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000032:analysis; D041121:Klebsiella oxytoca / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013638:Tars / Q000378:metabolism*; D018505:Waste Management / Q000379:methods
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D013638:Tars; C024746:tobacco tar