Sensing specific adhesion of liposomal and micellar systems with attached carbohydrate recognition structures at lectin surfaces.
A quartz crystal microbalance was used to investigate the adsorption behavior of liposomes and mixed micelles with attached carbohydrate recognition structures at lectin-coated quartz plates. With a self-assembly technique, the quartz was coated with the lectin Concanavalin A. In a first attempt, liposomes of natural soybean PC as well as synthetic POPC, containing 10% reactive N-Glut-PE each, were decorated with a mannopyranoside recognition structure to investigate the specific adsorption at the lectin-coated quartz surface in dependence on the concentration. In a second model, the bile salt sodium cholate was introduced to solubilize the mannopyranoside-modified liposomes and to transform them into mannopyranoside-modified binary mixed micelles. The adsorption of these micelles was further investigated. In a third approach, the adsorption behavior of mannopyranoside-modified ternary mixed bile salt-phosphatidylcholine-fatty acid micelles was characterized with sodium laurate, palmitate, and oleate as fatty acids. The micelles with oleate showed only a small frequency decrease, whereas the micelles with laurate and palmitate induced higher frequency changes. A dependence on the alkyl chain length could be detected. While the adsorption of liposomes containing recognition structures at QCM surfaces is nowadays well-established, the QCM detection of the adsorption of mixed bile salt micelles, transformed from these liposomes by solubilization, is a novel and very promising field for the development of innovative colloidal drug delivery systems.
Hildebrand
Annegret
A
Institute of Pharmaceutics and Biopharmaceutics, Martin-Luther-University Halle-Wittenberg, Wolfgang-Langenbeck-Str. 4, Halle/Saale D-06120, Germany.
Schaedlich
Anita
A
Rothe
Ulrich
U
Neubert
Reinhard H H
RH
D001647:Bile Acids and Salts / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry*; D003208:Concanavalin A / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry; D008823:Micelles; D013499:Surface Properties
D001647:Bile Acids and Salts; D002241:Carbohydrates; D005227:Fatty Acids; D037102:Lectins; D008081:Liposomes; D008823:Micelles; D003208:Concanavalin A; D008358:Mannose