title: Cross-linking chemistry and biology: development of multifunctional photoaffinity probes.
abstract: An efficient method of photoaffinity labeling has been developed based on rationally designed multifunctional photoprobes. Photoaffinity techniques have been used to elucidate the protein structure at the interface of biomolecules by the photochemical labeling of interacting sites. However, the identification of labeled sites within target proteins is often difficult. Novel biotinyl bioprobes bearing a diazirine photophore have contributed significantly to the rapid elucidation of ligand binding sites within proteins, thereby extending conventional photoaffinity methods. This article discusses the synthesis and applications of various photoprobes bearing a biotin, including strategies using cleavable linkages between photophores. The combination of photoaffinity methods with chip technology is also described as a novel entry to rapid affinity-based screening of inhibitors. This review focuses on a rapid and reliable photoaffinity method utilizing diazirine-based multifunctional photoprobes with numerous potential applications in functional proteomics of biomolecular interactions.
authors.affiliation: Laboratory of Biorecognition Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama, Toyama 930-0194, Japan.
authors.forename: Takenori
authors.initials: T
authors.lastname: Tomohiro
authors.forename: Makoto
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Yasumaru
authors.initials: Y
authors.lastname: Hatanaka
mesh_terms: D003978:Diazomethane / Q000737:chemistry; D019920:Photoaffinity Labels; D011487:Protein Conformation
chemical_list: D019920:Photoaffinity Labels; D003978:Diazomethane