Isolation and characterization of the promoter and partial enhancer region of the porcine inter-alpha-trypsin inhibitor heavy chain 4 gene.
A porcine genomic library was screened for clones containing the promoter of the major acute-phase protein in pigs, inter-alpha-trypsin heavy chain 4 (ITIH4). Following isolation of the promoter, a functional analysis was performed with Hep3B cells. The promoter was induced by interleukin-6 (IL-6) but not by IL-1beta. However, IL-1beta was shown to inhibit the IL-6-induced activation of the porcine ITIH4 promoter.
Harraghy
Niamh
N
Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, United Kingdom. bhnhar@uniklinik-saarland.de
Mitchell
Timothy J
TJ
D000209:Acute-Phase Proteins / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology; D015850:Interleukin-6 / Q000502:physiology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013552:Swine
D000209:Acute-Phase Proteins; D007375:Interleukin-1; D015850:Interleukin-6; C095815:ITIH4 protein, Sus scrofa