title: Development and use of antiproteasome monoclonal antibodies.
abstract: This chapter describes the production of hybridomas and screening of monoclonal antibodies for use in enzyme-linked immunosorbent assay (ELISA), immunoblotting, and immunoprecipitation of components of the ubiquitin-proteasome pathway. Purification of antibodies and their use in affinity chromatography are also described.
authors.affiliation: Institute of Molecular Biology and Physiology, University of Copenhagen, DK-2100 Copenhagen, Denmark.
authors.forename: Klavs B
authors.initials: KB
authors.lastname: Hendil
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D015925:Cryopreservation / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D015151:Immunoblotting; D007132:Immunoglobulin Isotypes; D051379:Mice; D008807:Mice, Inbred BALB C; D011092:Polyethylene Glycols; D046988:Proteasome Endopeptidase Complex / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes; D011092:Polyethylene Glycols; D046988:Proteasome Endopeptidase Complex