A comparison of cold storage solutions for pancreas preservation prior to islet isolation.
BACKGROUND
Several solutions are used to preserve the pancreas prior to islet isolation. This study sought to assess whether the type of solution had an impact on the isolation outcome.


METHODS
We reviewed data from 125 islet isolation procedures performed from January 2002 to January 2005. Pancreata were preserved in University of Wisconsin (UW) (n = 101), Celsior (CS) (n = 19), or IGL-1 (n = 5) solutions. Islet isolation results and transplantation rates were compared between groups.


RESULTS
UW, CS, and IGL-1 groups were similar according to donor's age, weight, and body mass index. Weight of undigested pancreas was 20 +/- 13.1, 21.4 +/- 15.7, and 17.4 +/- 8.7 g for UW, CS, and IGL-1, respectively (P > .2). Final total number of IEQ was 267,000 +/- 132,000, 277,000 +/- 155,000, and 311,000 +/- 163,000, respectively (P > .4). Success rate (defined as >250,000 IEQ) was 55.5%, 52.9%, and 60% for UW, Celsior, and IGL-1 (P > .9); the transplantation rate was 42.2% for UW, 36.8% for Celsior, and 80% for IGL-1 preservation (P > .2).


CONCLUSIONS
In this preliminary study, UW, Celsior, and IGL-1 solutions demonstrated similar islet isolation results. The new IGL-1 solution appears promising.
Wojtusciszyn
A
A
Surgery, Islet Isolation and Transplantation Center, 1 rue Michel Servet, 1211 Genèva 4, Switzerland. anne.wojtusciszyn@medcli.unige.ch
Bosco
D
D
Morel
Ph
P
Baertschiger
R
R
Armanet
M
M
Kempf
M C
MC
Badet
L
L
Toso
C
C
Berney
T
T
D000241:Adenosine; D000493:Allopurinol; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D005978:Glutathione; D006639:Histidine; D006801:Humans; D007328:Insulin; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation; D008353:Mannitol; D019188:Organ Preservation Solutions; D010179:Pancreas; D011887:Raffinose; D020858:Tissue and Organ Harvesting / Q000379:methods*
C088453:Celsior; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; C504757:IGL-1 solution; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D006639:Histidine; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose