Rapid and simple detection of blaCTX-M genes by multiplex PCR assay.
A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla(CTX-M) genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla(CTX-M) genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.
Xu
Li
L
Health Protection Agency West Midlands Public Health Laboratory, Birmingham Heartlands & Solihull NHS Trust, Birmingham B9 5SS, UK 2Division of Immunity and Infection, University of Birmingham, UK.
Ensor
Vicki
V
Health Protection Agency West Midlands Public Health Laboratory, Birmingham Heartlands & Solihull NHS Trust, Birmingham B9 5SS, UK 2Division of Immunity and Infection, University of Birmingham, UK.
Gossain
Savita
S
Health Protection Agency West Midlands Public Health Laboratory, Birmingham Heartlands & Solihull NHS Trust, Birmingham B9 5SS, UK 2Division of Immunity and Infection, University of Birmingham, UK.
Nye
Kathy
K
Health Protection Agency West Midlands Public Health Laboratory, Birmingham Heartlands & Solihull NHS Trust, Birmingham B9 5SS, UK 2Division of Immunity and Infection, University of Birmingham, UK.
Hawkey
Peter
P
Health Protection Agency West Midlands Public Health Laboratory, Birmingham Heartlands & Solihull NHS Trust, Birmingham B9 5SS, UK 2Division of Immunity and Infection, University of Birmingham, UK.
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; D017931:DNA Primers; D001618:beta-Lactamases