Nanobiolistic delivery of indicators to the living mouse retina.
The development of a technique to load functional indicators into living neurons is an ongoing challenge in retinal neurophysiology. In a number of live-cell preparations, fluorescence-based indicators have been of particular importance for investigating ionic concentrations, protein localization, and other physiological parameters. In the present study, we demonstrate a novel technique that uses a modified gene gun to propel silver nanoparticles coated with indicators into live retinal neurons, and we highlight the advantages of using this technique to deliver these functional indicators.
Roizenblatt
Roberto
R
Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, CA, USA. rrtt@uol.com.br
Weiland
James D
JD
Carcieri
Stephen
S
Qiu
Guanting
G
Behrend
Matthew
M
Humayun
Mark S
MS
Chow
Robert H
RH
D000818:Animals; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005456:Fluorescent Dyes; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D010316:Particle Size; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000502:physiology*
D005456:Fluorescent Dyes