Rye SCAR markers for male fertility restoration in the P cytoplasm are also applicable to marker-assisted selection in the C cytoplasm.
The study aimed at testing the usefulness of recently developed SCAR markers on rye (Secale cereale L.) chromosome 4R in hybrid breeding based on the C source of male sterility-inducing cytoplasm. Of 10 markers studied, 4 revealed polymorphisms between 2 inbred lines (544cms-C and Ot0-20) crossed to develop F2 and BC1 mapping populations. Analyses performed on 94 F2 and 93 BC1 plants allowed to extend a formerly constructed genetic map of chromosome arm 4RL. Three SCAR markers (SCP14M55, SCP15M55 and SCP16M58) were mapped in the vicinity of gene Rfc1, which restores male fertility in the C cytoplasm. The 3 tested SCAR markers proved to be effective in marker-assisted selection (MAS) for male fertility/sterility.
Stojałowski
Stefan
S
Department of Genetics and Plant Breeding, Agricultural University, Slowackiego 17, 71-437 Szczecin, Poland. stefs@agro.ar.szczecin.pl
Jaciubek
Milosława
M
Masojć
Piotr
P
D001947:Breeding / Q000379:methods*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003593:Cytoplasm / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012098:Reproduction / Q000235:genetics; D012434:Secale / Q000235:genetics*
D005819:Genetic Markers