[Gene cloning of rpoD and its impact on biosynthesis of antibiotics in Fluorescent pseudomonas M18].
Fluorescent pseudomonas M18, one of plant growth promoting rhizobacteria which can inhibit growth of several phytopathogenes, produces several secondary metabolites including antibiotics phenazine-1-carboxylic acid (PCA) and pyoluteorin (Plt). The rpoD gene encoding the housekeeping sigma factor sigma70 was cloned and sequenced from M18. Through sequencing and homogeneous comparison, the deduced RpoD amino acid sequence between M18 strain and Pseudomonasfluorescens CHAO shows 100% identity. It indicates that rpoD gene is very conserved in different members of fluorescent pseudomonads. The rpoD gene was placed downstream of the constitutive Ptac promoter in the shuttle vector pME6032 between E. coli and Pseudomonas fluorescens and the recombinant plasmid was introduced into M18. It was found that the time of both PCA and Plt began to accumulate was 4 and 8 hours earlier and the yield of these antibiotics was increased one and six times more respectively in M18 in comparison with the control.
Zhu
Donghua
D
School of Life Science & Biotechnology, Shanghai JiaoTong University, Shanghai 200240, China.
Xu
Wangjie
W
Geng
Haifeng
H
Zhang
Xuehong
X
Xu
Yuquan
Y
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005453:Fluorescence; D008969:Molecular Sequence Data; D011551:Pseudomonas fluorescens / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D012808:Sigma Factor / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D012808:Sigma Factor