Leadbetterella byssophila gen. nov., sp. nov., isolated from cotton-waste composts for the cultivation of oyster mushroom.
A bacterial strain, designated 4M15T, was isolated from cotton-waste composts used as mushroom cultivation in South Korea. Properties of this isolate were studied on the basis of physiological and biochemical characteristics, fatty acid profile, isoprenoid quinone, DNA G+C content and phylogenetic position based on 16S rRNA gene sequence analysis. The strain was found to form a distinct phylogenetic lineage related to the family 'Flexibacteraceae' within the phylum 'Bacteroidetes'. No recognized species showed >85 % 16S rRNA gene sequence similarity to strain 4M15T. The fatty acid profile of strain 4M15(T) included C(16 : 1)omega7c/iso-C(15 : 0) 2-OH (30.5 %), iso-C(15 : 0) (24.2 %), iso-C(15 : 0) 2-OH/C(16 : 1)omega7c (15.9), iso-C(17 : 0) 3-OH (10.5 %) and C(16 : 0) (5.6 %). The major isoprenoid quinone was menaquinone MK-7. The DNA G+C content was 33.0 mol%. Cells were Gram-negative, strictly aerobic, rod-shaped, non-motile, catalase-positive, oxidase-positive and flexirubin-positive. The strain hydrolysed aesculin, gelatin, starch and tyrosine. Several phenotypic tests could be used to differentiate strain 4M15T from other members of the family 'Flexibacteraceae'. On the basis of the data presented, strain 4M15T should be assigned to the phylum 'Bacteroidetes' as a novel genus and species, for which the name Leadbetterella byssophila gen. nov., sp. nov. is proposed. The type strain is 4M15T (=KACC 11308T=DSM 17132T).
Weon
Hang-Yeon
HY
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration (RDA), Suwon 441-707, Republic of Korea.
Kim
Byung-Yong
BY
Genetic Resources Division, Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
Kwon
Soon-Wo
SW
Genetic Resources Division, Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
Park
In-Cheol
IC
Microbial Function Team, Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
Cha
In-Bae
IB
Genetic Resources Division, Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
Tindall
Brian J
BJ
Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Stackebrandt
Erko
E
Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany.
Trüper
Hans G
HG
Institut für Mikrobiologie and Biotechnologie, Universität Bonn, Meckenheimer Allee 168, D-53115 Bonn, Germany.
Go
Seung-Joo
SJ
Genetic Resources Division, Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Biotechnology, RDA, Suwon 441-707, Republic of Korea.
D000818:Animals; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D003368:Gossypium / Q000382:microbiology; D007220:Industrial Waste / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D020076:Pleurotus / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S