Toxoplasma gondii: Serological recognition of reinfection.
Using murine chronic toxoplasmosis as an experimental model, we examined the utility of immunoenzymatic methods in recognizing reinfection in chronically infected individuals. Primary infection with avirulent Toxoplasma gondii DX strain (genotype II) induced strong immunity protecting the mice from mortality after inoculation with LD(100) of virulent BK strain (genotype I) and triggered highly expressed antibody production, within one new isotype detected by comparative immunoblots. The parasites multiplying at the site of reinfection were of BK origin as found by RAPD-PCR. The results revealed that the immunoblot assay seems to be a useful and reliable method for the monitoring of specific antibody profile in chronically infected individuals. In our opinion ELISA combined with immunoblot could enable the recognition of reinfection cases in humans, but earlier our experimental data should be verified in clinical laboratory.
Dzitko
Katarzyna
K
Department of Immunoparasitology, University of Lodz, Poland. dzika@biol.uni.lodz.pl
Staczek
Pawel
P
Gatkowska
Justyna
J
Dlugonska
Henryka
H
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001202:Ascitic Fluid / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D007121:Immunocompetence; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019105:Random Amplified Polymorphic DNA Technique; D012008:Recurrence; D014122:Toxoplasma / Q000276:immunology* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000175:diagnosis / Q000276:immunology*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M