title: Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122.
abstract: A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6(1)22 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl(2) or MnCl(2) results in full occupancy of the dinuclear metal site. The structure of the Mn(II)-loaded form was determined to 2.6 Angstroms resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.
authors.affiliation: Department of Biochemistry, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Monika
authors.initials: M
authors.lastname: Sommerhalter
authors.forename: Lana
authors.initials: L
authors.lastname: Saleh
authors.forename: J Martin
authors.initials: JM
authors.lastname: Bollinger
authors.forename: Amy C
authors.initials: AC
authors.lastname: Rosenzweig
mesh_terms: D001665:Binding Sites; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology; D008958:Models, Molecular; D011487:Protein Conformation; D012264:Ribonucleotide Reductases / Q000737:chemistry*
chemical_list: D012264:Ribonucleotide Reductases; C108427:ribonucleotide reductase R2 subunit