[The expression of VP19 gene from prawn white spot syndrome virus in silkworm, Bombyx mori using host range-expanded HyNPV].
Prawn white spot syndrome is caused by the pathogen prawn white spot syndrome virus (WSSV). VP19 is a vesicle membrane protein of WSSV. HyNPV (Hybrid of AcNPV and BmNPV) constructed by the recombination of BmNPV and AcNPV is a new hybrid virus having both of their advantages. The recombinant transfer vector pBlueBicHisC-vp19 and recombinant baculovirus HyNPV-VP19 were constructed on the basis of the successful cloning of VP19. Newly-molted silkworms Bombyx mori of fifth instar were inoculated by the recombinant virus. SDS-PAGE and Western blotting analysis showed a specific band, about 21kD, which was consistent with the expectation suggesting that the WSSV-VP19 gene was successfully expressed in silkworm bodies.
Xu
Ya-Xiang
YX
Suzhou University, Suzhou 215006, China. szdxxyx@163.com
Wu
Xiao-Feng
XF
Zhu
Yu-Fang
YF
Xu
Zi-Rong
ZR
Shen
Wei-De
WD
D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D012831:Bombyx / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D005822:Genetic Vectors; D033561:Penaeidae / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication; D046848:White spot syndrome virus 1 / Q000235:genetics*
D011993:Recombinant Fusion Proteins; C446941:VP19 protein, white spot syndrome virus; D014759:Viral Envelope Proteins