Human, rhesus macaque, and feline sequences highly similar to mouse mammary tumor virus sequences.
Sequences highly similar (>95%) to the mouse mammary tumor virus (MMTV) env gene have been amplified from human DNA samples, including DNA samples from patients with breast cancer (BC) and persons who did not have BC. The sequences from human DNA were distinct from the MMTV sequences used as controls in these PCR reactions, indicating that these results are not simply due to contamination. In addition to both, mouse and human-related sequences were also amplified from some monkey and cat genomic DNA samples. These products were shown to be distinct from, but highly related to, the MMTV env gene, whereas, testing of other sources (lambda phage, snake, cockroach, sea urchin, chicken, or dog) demonstrated no specific amplification. A sequence 90% similar to the MMTV group antigen gene (gag) was amplified from cat DNA. These results indicate that DNA from vertebrate species other than rodents, including some but not all humans, monkeys, and cats, can contain sequences closely related to MMTV.
Szabo
Sara
S
Department of Pathology, Children's Hospital, New Orleans, Louisiana 70118, USA. sszabo@lsuhsc.edu
Haislip
Allyson M
AM
Traina-Dorge
Vicki
V
Costin
Joshua M
JM
Crawford
Byron E
BE
Wilson
Russell B
RB
Garry
Robert F
RF
D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000821:virology*; D002415:Cats; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015752:Genes, env; D006801:Humans; D008253:Macaca mulatta; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D004273:DNA, Neoplasm; D004279:DNA, Viral