title: Miniaturized thermal lens and fluorescence detection system for microchemical chips.
abstract: We have developed a miniaturized two-way detection system using thermal lens and fluorescence spectroscopies for microchip chemistry. The system was composed of laser diode (LD) modules, fiber-based optics combined with a gradient index lens, and miniaturized detection units for thermal lens and fluorescence signals. The detection limits in the thermal lens and fluorescence spectroscopies were 6.3 x 10(-9)M for Ni(II) phthalocyanine tetrasulfonic acid and 3.0 x 10(-9)M for cy5, respectively. The performance of the system with the miniaturized thermal lens was equivalent to that of a conventional thermal lens microscope. The fluorescence sensitivity was comparable to sensitivities offered by conventional miniaturized systems.
authors.affiliation: Institute of Microchemical Technology, 3-2-1 Sakado, Takatsu, Kawasaki 213-0012, Japan.
authors.forename: Masayo
authors.initials: M
authors.lastname: Yamauchi
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
authors.forename: Jun
authors.initials: J
authors.lastname: Yamaguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Fukuzawa
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hattori
authors.forename: Akihide
authors.initials: A
authors.lastname: Hibara
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kitamori
mesh_terms: D008904:Miniaturization; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature