title: [Study on breeding up high-yield strain of taxol by protoplast mutagensis].
abstract: In order to obtain resistant mutants to nystatin, ultraviolet radiation and LiCl were used to mutagenize the protoplasts of taxol-producing fungi NCEU-1, and four positive mutants with high yield of taxol were screened out on nystatin flat. After further screening experiments on fermentation, a mutant strain--UL04-5 which was able to produce taxol with high yield and could be stably passed on in genetics was eventually found, it's ability to produce taxol was improved from 314.07 microg/L (strain NCEU-1) to 418.24 microg/L (strain U04-5).
authors.affiliation: Life and Science College of Heilongjiang University, Harbin 150080, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Dong-Po
authors.initials: DP
authors.lastname: Zhou
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: Ping
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Xi
authors.initials: X
authors.lastname: Ma
authors.forename: Tao
authors.initials: T
authors.lastname: Jin
mesh_terms: D001203:Ascomycota / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D014644:Genetic Variation; D016296:Mutagenesis; D009761:Nystatin / Q000494:pharmacology; D017239:Paclitaxel / Q000096:biosynthesis*; D011523:Protoplasts / Q000378:metabolism*
chemical_list: D009761:Nystatin; D017239:Paclitaxel