title: Electron spin resonance assay of ascorbyl radical generation in mouse hippocampal slices during and after kainate-induced seizures.
abstract: As an index of oxidative status, we analyzed ascorbyl radical generation during and after kainate-induced seizures in mouse hippocampus, using an ESR spectrometer equipped with a special tissue-type quartz cell. A specific doublet ESR spectrum was observed after seizures, and the g value and the hyperfine coupling constant (hfcc) of the spectrum were identical with those of ascorbyl radical itself. Antiepileptic zonisamide inhibited the generation of ascorbyl radical accompanying the seizures.
authors.affiliation: JEOL, Ltd., Tokyo 196-8558 and Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan. masumizu@ph.sojo-u.ac.jp
authors.forename: Toshiki
authors.initials: T
authors.lastname: Masumizu
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Noda
authors.forename: Akitane
authors.initials: A
authors.lastname: Mori
authors.forename: Lester
authors.initials: L
authors.lastname: Packer
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000032:analysis* / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D004830:Epilepsy, Tonic-Clonic / Q000139:chemically induced / Q000378:metabolism; D018690:Excitatory Amino Acid Agonists; D005609:Free Radicals / Q000032:analysis* / Q000378:metabolism*; D006624:Hippocampus / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D007555:Isoxazoles / Q000494:pharmacology; D007608:Kainic Acid; D008297:Male; D017895:Manganese Compounds / Q000737:chemistry; D051379:Mice; D010087:Oxides / Q000737:chemistry; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D000078305:Zonisamide
chemical_list: D000927:Anticonvulsants; D018690:Excitatory Amino Acid Agonists; D005609:Free Radicals; D007555:Isoxazoles; D017895:Manganese Compounds; D010087:Oxides; D000078305:Zonisamide; C027424:manganese oxide; D001205:Ascorbic Acid; D007608:Kainic Acid