Cloning and functional analysis of the gene encoding the 33- to 36-kilodalton outer membrane protein associated with carbapenem resistance in Acinetobacter baumannii.
We investigated a multiresistant strain of Acinetobacter baumannii isolated in our hospital. Analysis of the N-terminal peptide sequence of the outer membrane proteins (OMPs) purified from the strain allowed us to clone and sequence the nucleotides of the gene encoding the 33- to 36-kDa OMP associated with carbapenem resistance in A. baumannii.
del Mar Tomás
Maria
M
Servicio de Microbiologia-Unidad de Investigacion, Complejo Hospitalario Universitario Juan Canalejo, As Xubias, 84, 15006 La Coruña, Spain.
Beceiro
Alejandro
A
Pérez
Astrid
A
Velasco
David
D
Moure
Rita
R
Villanueva
Rosa
R
Martínez-Beltrán
Jesús
J
Bou
Germán
G
D000150:Acinetobacter / Q000145:classification / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D015780:Carbapenems / Q000378:metabolism / Q000494:pharmacology*; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight
D001425:Bacterial Outer Membrane Proteins; D015780:Carbapenems