[Correlation between the content of transforming growth factor-beta 1, interleukin-1 beta and collagenase activity in proximal femur bone samples obtained from patients with femoral neck fracture].
The aim of this study was to determine correlations between the content of TGF-beta1, IL-1beta and collagenase activity in bone samples of the femoral neck obtained from patients with femoral neck fracture. The material consisted of 42 samples of cancellous bone of femoral neck collected during hemiarthroplasty or total hip replacement procedure. 24 samples of cancellous bone from patients with osteoarthritis of the hip harvested during total hip replacement served as a control group. The content of TGF-beta1, IL-1beta was measured using ELISA and collagenase activity was measured with spectrofluorometry. In patients with fracture there was found a distinct inversely proportional correlation between the content of TGF-beta1 and IL-1beta. This correlation was not observed in the control group. In addition, a directly proportional relation between the content of TGF-beta1 and IL-1beta was discovered. These results confirm mutual correlations between examined cytokines in bone.
Zgoda
Marcin
M
Katedra i Klinika Ortopedii i Traumlatologii Narzaclu Ruchu, Akademia Medyczna w Warszawie.
Chmielewski
Dariusz
D
Paczek
Leszek
L
Bartłomiejczyk
Irena
I
Ambroziak
Maciej
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001842:Bone and Bones / Q000201:enzymology / Q000378:metabolism*; D016022:Case-Control Studies; D017364:Collagenases / Q000378:metabolism*; D005260:Female; D005265:Femoral Neck Fractures / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1
D007375:Interleukin-1; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D017364:Collagenases