Intestinal absorption and metabolism of a soluble flavonoid, alphaG-rutin, in portal cannulated rats.
A highly soluble quercetin glycoside, alphaG-rutin, is a glucose adduct of insoluble rutin, and intestinal absorption and metabolism of alphaG-rutin has not been known. We investigated the intestinal absorption and metabolism of alphaG-rutin by using portal and duodenal cannulated rats and the isolated rat intestinal mucosa. After a duodenal instillation of alphaG-rutin (150 mumol), intact alphaG-rutin, rutin and quercetin were appeared in the portal blood and these concentrations were similarly increased at 15 min. Portal quercetin reached a peak value at 60 min, and the value was higher than those of alphaG-rutin and rutin at that time. Quercetin-conjugates were also increased 30 min after the instillation. The remaining of alphaG-rutin metabolites, mainly rutin, in the intestine were 58% of instilled alphaG-rutin after 150 min. In the experiment by using the isolated mucosa of the jejunum, ileum and cecum, alphaG-rutin and rutin, but not quercetin, appeared in the serosal sides of all segments, and they were increased linearly from 10 to 100 mmol/l of mucosal alphaG-rutin. We also showed portal injected alphaG-rutin was very rapidly cleared from the blood, and appeared a large amount of conjugates. In conclusion, a soluble flavonoid-glycoside, alphaG-rutin, was absorbed as glycosides into the portal blood. A part of alphaG-rutin was hydrolyzed to rutin, but not to aglycone, through the intestine.
Matsumoto
Mgumi
M
Fuji Women's University, Faculty of Human Life Science, Department of Food Science and Human Nutrition, Ishikari, Hokkaido, 061-3204, Japan.
Chiji
Hideyuki
H
Hara
Hiroshi
H
D000818:Animals; D001692:Biological Transport; D002404:Catheterization; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006868:Hydrolysis; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008297:Male; D015394:Molecular Structure; D011169:Portal Vein / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D014312:Trisaccharides
D014312:Trisaccharides; C069037:4(G)-alpha-glucopyranosyl-rutin; D012431:Rutin