title: Genetic mapping of Eutr1, a locus controlling E2-induced pyometritis in the Brown Norway rat, to RNO5.
abstract: In certain rat strains, chronic estrogen administration can lead to pyometritis, an inflammation of the uterus accompanied by infection and the accumulation of intraluminal pus. In this article, we report that the Brown Norway (BN) rat is highly susceptible to pyometritis induced by 17beta-estradiol (E2). The susceptibility of the BN rat to E2-induced pyometritis appears to segregate as a recessive trait in crosses to the resistant August x Copenhagen Irish (ACI) strain. In a (BN x ACI)F(2) population, we find strong evidence for a major genetic determinant of susceptibility to E2-induced pyometritis on rat chromosome 5 (RNO5). Our data are most consistent with a model in which the BN allele of this locus, designated Eutr1 (Estrogen-induced uterine response 1), acts in an incompletely dominant manner to control E2-induced pyometritis. Furthermore, we have confirmed the contribution of Eutr1 to E2-induced uterine pyometritis using an RNO5 congenic rat strain. In addition to Eutr1, we obtained evidence suggestive of linkage for five additional loci on RNO2, 4, 11, 17, and X that control susceptibility to E2-induced pyometritis in the (BN x ACI)F(2) population.
authors.affiliation: Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA. kagould@unmc.edu
authors.forename: Karen A
authors.initials: KA
authors.lastname: Gould
authors.forename: Jyotsna
authors.initials: J
authors.lastname: Pandey
authors.forename: Cynthia M
authors.initials: CM
authors.lastname: Lachel
authors.forename: Clare R
authors.initials: CR
authors.lastname: Murrin
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Flood
authors.forename: Karen L
authors.initials: KL
authors.lastname: Pennington
authors.forename: Beverly S
authors.initials: BS
authors.lastname: Schaffer
authors.forename: Martin
authors.initials: M
authors.lastname: Tochacek
authors.forename: Rodney D
authors.initials: RD
authors.lastname: McComb
authors.forename: Jane L
authors.initials: JL
authors.lastname: Meza
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Wendell
authors.forename: James D
authors.initials: JD
authors.lastname: Shull
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics*; D004716:Endometritis / Q000139:chemically induced* / Q000235:genetics*; D004958:Estradiol / Q000633:toxicity*; D005260:Female; D020022:Genetic Predisposition to Disease; D008297:Male; D051381:Rats; D011912:Rats, Inbred ACI; D011914:Rats, Inbred BN / Q000235:genetics*
chemical_list: D004958:Estradiol