The differential gene expression profiles of proximal and distal muscle groups are altered in pre-pathological dysferlin-deficient mice.
The selective pattern of muscle involvement is a key feature of muscular dystrophies. Dysferlinopathy is a good model for studying this process since it shows variable muscle involvement that can be highly selective even in individual patients. The transcriptomes of proximal and distal muscles from wildtype C57BL/10 and dysferlin deficient C57BL/10.SJL-Dysf mice at a prepathological stage were assessed using the Affymetrix oligonucleotide-microarray system. We detected significant variation in gene expression between proximal and distal muscle in wildtype mice. Dysferlin defiency, even in the absence of pathological changes, altered this proximal distal difference but with little specific overlap with previous microarray analyses of dysferlinopathy. In conclusion, proximal and distal muscle groups show distinct patterns of gene expression and respond differently to dysferlin deficiency. This has implications for the selection of muscles for future microarray analyses, and also offers new routes for investigating the selectivity of muscle involvement in muscular dystrophies.
von der Hagen
Maja
M
Institute of Human Genetics, University Newcastle upon Tyne, UK.
Laval
Steven H
SH
Cree
Lynsey M
LM
Haldane
Faye
F
Pocock
Matthew
M
Wappler
Ilka
I
Peters
Heiko
H
Reitsamer
Herbert A
HA
Hoger
Harald
H
Wiedner
Maria
M
Oberndorfer
Felicitas
F
Anderson
Louise V B
LV
Straub
Volker
V
Bittner
Reginald E
RE
Bushby
Kate M D
KM
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D040502:Calgranulin B / Q000378:metabolism; D004195:Disease Models, Animal; D000073939:Dysferlin; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods; D008565:Membrane Proteins / Q000172:deficiency* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018345:Mice, Knockout; D046228:Microarray Analysis / Q000379:methods; D009124:Muscle Proteins / Q000172:deficiency* / Q000502:physiology; D018482:Muscle, Skeletal / Q000201:enzymology / Q000473:pathology; D009136:Muscular Dystrophies / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
D040502:Calgranulin B; C114839:DYSF protein, human; D000073939:Dysferlin; D008565:Membrane Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger