Effects of L-alanyl-glutamine upon the blood and kidney biochemical parameters in the rat hind limb model of ischemia/reperfusion.
OBJECTIVE
To investigate the effects of l-alanyl-glutamine (Ala-Gln) intragastric administration upon blood and kidney metabolic parameters alterations in rats subjected to ischemia/reperfusion of hind limb.


METHODS
Forty-eight male rats were randomized in 2 groups offered via gavage either saline 2.0 mL (G-1) or Ala-Gln solution 0.75 mgKg-1(G-2) once a day at 7 AM during 7 days. One-hour after the last gavage (Day 7) all rats were submitted to ether anesthesia, laparotomy and clamping of the left iliac artery for 3 h. Kidney and blood samples were collected at the end of ischemic period (3 h) and at 1-3-6 h during reperfusion period for metabolites (pyruvate, lactate, glucose and ketone bodies) enzymatic analysis. ATP was also assayed in kidney samples.


RESULTS
Lactacemia and ketonemia were significantly increased in Ala-Gln treated rats during reperfusion. Kidney pyruvate concentrations were significantly decreased and tissue lactate concentrations were significantly increased during reperfusion (1 h and 3 h) in G-2 rats compared with respective controls. Glucose, ATP and ketone bodies concentrations were significantly increased in the kidney in L-Ala-Gln treated rats at 3 hours after reperfusion as compared to respective controls.


CONCLUSIONS
Unilateral hind limb ischemia in L-Ala-Gln pre-treated rats may induce increased lactacemia and increased kidney lactate concentrations, indicating increased glycolytic activity in renal medulla and in other peripheral tissues. Higher ketonemia during reperfusion may reflect a possible increase in ketogenesis due to lower insulin plasma concentration hepatic signaling as a result of increased glucose oxidation in peripheral tissues, caused by the intra-gastric administration of glutamine dipeptide, suggesting also decreased insulin resistance.
Alves
Marcos Antonio
MA
Federal University of Ceará (UFC), Brazil.
Guimarães
Sérgio Botelho
SB
Dias
Daniel Aguiar
DA
Vasconcelos
Paulo Roberto Cavalcante de
PR
Coelho
Vicente de Paulo Martins
Vde P
Vasconcelos
Paulo Roberto Leitão de
PR
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D004151:Dipeptides / Q000494:pharmacology*; D004195:Disease Models, Animal; D006614:Hindlimb / Q000098:blood supply* / Q000378:metabolism; D007511:Ischemia / Q000209:etiology / Q000378:metabolism; D007659:Ketones / Q000097:blood; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D019344:Lactic Acid / Q000097:blood; D008297:Male; D019289:Pyruvic Acid / Q000097:blood; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000378:metabolism*; D018709:Statistics, Nonparametric
D001786:Blood Glucose; D004151:Dipeptides; D007659:Ketones; D019344:Lactic Acid; D019289:Pyruvic Acid; C054122:alanylglutamine