A region C-terminal to the proline-rich core of p47phox regulates activation of the phagocyte NADPH oxidase by interacting with the C-terminal SH3 domain of p67phox.
Activation of the phagocyte NADPH oxidase requires the regulatory proteins p47(phox) and p67(phox), each harboring two SH3 domains. p67(phox) interacts with p47(phox) via simultaneous binding of the p67(phox) C-terminal SH3 domain to both the proline-rich region (PRR) of amino acid residues 360-369 and its C-terminally flanking region of p47(phox); the role of the interaction in oxidase regulation has not been fully understood. Here we show that the p47(phox)-p67(phox) interaction is disrupted not only by deletion of the PRR but also by substitution for basic residues in the extra-PRR (K383E/K385E). The substitution impaired oxidase activation partially in vitro and much more profoundly in vivo, indicating the significance of the p47(phox) extra-PRR. Replacement of Ser-379 in the extra-PRR, a residue known to undergo phosphorylation in stimulated cells, by aspartate attenuates the interaction and thus results in a defective superoxide production, suggesting that phosphorylation of Ser-379 is involved in oxidase regulation.
Mizuki
Kazuhito
K
Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.
Takeya
Ryu
R
Kuribayashi
Futoshi
F
Nobuhisa
Ikuo
I
Kohda
Daisuke
D
Nunoi
Hiroyuki
H
Takeshige
Koichiro
K
Sumimoto
Hideki
H
D019943:Amino Acid Substitution; D002478:Cells, Cultured; D004789:Enzyme Activation; D006801:Humans; D019255:NADPH Oxidases / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology*; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D011392:Proline / Q000737:chemistry / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*; D017434:Protein Structure, Tertiary; D013329:Structure-Activity Relationship
D010750:Phosphoproteins; C079789:neutrophil cytosol factor 67K; D011392:Proline; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1