Development of synthetic peptide ELISA based on nonstructural protein 2C of foot and mouth disease virus.
It was reported that the sera of convalescent animals contain antibodies to foot and mouth disease (FMD) virus (FMDV) 2C, highly conserved nonstructural protein (NSP), whereas the sera of vaccinated animals do not. But ELISA methods using this protein were not reported and developed until recently. In this study, NSP 2C peptides were synthesized within the amino acid sequence of the conserved 2C nonstructural region of FMDV according to the sequences from Genbank database and used for identifying antigenic determinants. One of the synthesized thirteen peptides gave strong positive reactivity with most of the sera from 13 FMD infected farms, but not with sera from vaccinated and non-infected animals. Moreover, with the sera collected through serial bleedings from four cattle and five goats infected with FMDV O/SKR/2000 experimentally, positive results were obtained in two species after 10 days post infection (DPI). Therefore, we tried to develop and evaluate this ELISA based on 2C peptides. In comparison with the commercial NSP ELISA, the 2C peptide based ELISA method showed good specificity and sensitivity. These results demonstrate that the synthetic 2C peptide ELISA can be a complementary marker to differentiate FMDV-infected from vaccinated on a herd basis.
Oem
Jae Ku
JK
National Veterinary Research and Quarantine Service, Ministry of Agriculture, Anyang 430-824, Korea. jku0622@mail.nvrqs.go.kr
Kye
Soo Jeong
SJ
Lee
Kwang Nyeong
KN
Park
Jong Hyeon
JH
Kim
Yong Joo
YJ
Song
Hee Jong
HJ
Yeh
Max
M
D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000175:diagnosis*; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification*; D006041:Goats; D012680:Sensitivity and Specificity; D014611:Vaccination; D017361:Viral Nonstructural Proteins / Q000138:chemical synthesis*; D014765:Viral Vaccines
D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines