Membrane HSP70: the molecule triggering gammadelta T cells in the early stage of tumorigenesis.
Many studies support the supposition that HSPs expressed on the cell membrane play an important role in cancer immunity. In the present study, we demonstrated that HSP60 and HSP70 are markedly increased on the cell membrane of human Epstein-Barr virus (EBV) transformed B cells. In order to investigate whether these molecules were involved in the response of human gammasigma T cells to transformed cells, the cytotoxicities of gammasigma T cells to transformed cells with or without an HSP60/70 gene knockdown were evaluated. gammasigma T cells showed marked cytotoxities to transformed cells. Down-regulation of HSP70 expression could inhibit the reactions, whereas down-regulation of HSP60 expression had little such effect. Moreover, HSP72 could significantly induce human gammasigma T cells to proliferate in vitro. Taken together, our data indicated that HSP60 and HSP70 could be valuable biomarkers for the prediction of early stage in tumorigenesis. Additionally, HSP72 might be a potential candidate of the adjuvant for gammasigma T cells in tumor immunotherapy.
Zhang
Huiyuan
H
Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, China.
Hu
Hongbo
H
Jiang
Xinxin
X
He
Hongbin
H
Cui
Lianxian
L
He
Wei
W
D001402:B-Lymphocytes / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000276:immunology*; D018834:Chaperonin 60 / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D015536:Down-Regulation; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D050884:HSP72 Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism; D004854:Herpesvirus 4, Human; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D015415:Biomarkers; D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; D008565:Membrane Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta