The measurement of nitric oxide production by cultured endothelial cells.
Nitric oxide (NO) produced by vascular endothelial cells (ECs) plays a critical role in normal vascular physiology. Important insights into mechanisms regulating the production of endothelial NO have been derived from in vitro studies employing cultured ECs. Although many techniques for the detection of NO have been described, many of these methods lack adequate sensitivity to detect the small amount of NO produced by cultured ECs. In this chapter, we describe three protocols that employ chemiluminescence, electron spin resonance, or electrochemical techniques to permit the reliable detection of EC NO production.
Hart
C Michael
CM
Department of Medicine, Emory University, Atlanta VA Medical Center, Decatur, Georgia 30033, USA.
Kleinhenz
Dean J
DJ
Dikalov
Sergey I
SI
Boulden
Beth M
BM
Dudley
Samuel C
SC
D002138:Calibration; D002478:Cells, Cultured; D004563:Electrochemistry; D004578:Electron Spin Resonance Spectroscopy; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D008163:Luminescent Measurements; D009569:Nitric Oxide / Q000096:biosynthesis*
D009569:Nitric Oxide