Determination of the hydrolysis rate constants and activation energy of aesculin with capillary electrophoresis end-column amperometric detection.
Aesculetin is the product of the hydrolysis reaction of aesculin. A high sensitivity and good repeatability method based on capillary electrophoresis with amperometric detection (CE-AD) was developed for simultaneous determination of aesculin and aesculetin in the hydrolysate of aesculin. Under the optimum condition: 10mmol/L KH(2)PO(4)-5mmol/L Na(2)B(4)O(7) (pH 6.0) buffer, separation at 18kV and +900mV (versus Ag/AgCl) as the detection potential, the hydrolysis rate constants of aesculin hydrolysis at 25, 30, 35, 40 and 45 degrees C in 0.1mol/L KOH were obtained as 1.45x10(-2)min(-1), 2.01x10(-2)min(-1), 2.93x10(-2)min(-1), 3.76x10(-2)min(-1) and 5.05x10(-2)min(-1), respectively. It was calculated that the activation energy for aesculin hydrolysis was 49.4kJ/mol.
Zhang
Lan
L
The Sport Science Research Center, Fuzhou University, Fuzhou, Fujian 350002, China. zlan@fzu.edu.cn
Tong
Ping
P
Chen
Guonan
G
D002021:Buffers; D004563:Electrochemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D004929:Esculin / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014468:Umbelliferones / Q000737:chemistry* / Q000378:metabolism
D002021:Buffers; D014468:Umbelliferones; D004929:Esculin; C007628:esculetin