title: Oxidative mediated lipid peroxidation recapitulates proarrhythmic effects on cardiac sodium channels.
abstract: Sudden cardiac death attributable to ventricular tachycardia/fibrillation (VF) remains a catastrophic outcome of myocardial ischemia and infarction. At the same time, conventional antagonist drugs targeting ion channels have yielded poor survival benefits. Although pharmacological and genetic models suggest an association between sodium (Na+) channel loss-of-function and sudden cardiac death, molecular mechanisms have not been identified that convincingly link ischemia to Na+ channel dysfunction and ventricular arrhythmias. Because ischemia can evoke the generation of reactive oxygen species, we explored the effect of oxidative stress on Na+ channel function. We show here that oxidative stress reduces Na+ channel availability. Both the general oxidant tert-butyl-hydroperoxide and a specific, highly reactive product of the isoprostane pathway of lipid peroxidation, E2-isoketal, potentiate inactivation of cardiac Na+ channels in human embryonic kidney (HEK)-293 cells and cultured atrial (HL-1) myocytes. Furthermore, E2-isoketals were generated in the epicardial border zone of the canine healing infarct, an arrhythmogenic focus where Na+ channels exhibit similar inactivation defects. In addition, we show synergistic functional effects of flecainide, a proarrhythmic Na+ channel blocker, and oxidative stress. These data suggest Na+ channel dysfunction evoked by lipid peroxidation is a candidate mechanism for ischemia-related conduction abnormalities and arrhythmias.
authors.affiliation: Department of Anesthesiology, Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: Koji
authors.initials: K
authors.lastname: Fukuda
authors.forename: Sean S
authors.initials: SS
authors.lastname: Davies
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nakajima
authors.forename: Boon-Hooi
authors.initials: BH
authors.lastname: Ong
authors.forename: Sabina
authors.initials: S
authors.lastname: Kupershmidt
authors.forename: Joshua
authors.initials: J
authors.lastname: Fessel
authors.forename: Venkataraman
authors.initials: V
authors.lastname: Amarnath
authors.forename: Mark E
authors.initials: ME
authors.lastname: Anderson
authors.forename: Penelope A
authors.initials: PA
authors.lastname: Boyden
authors.forename: Prakash C
authors.initials: PC
authors.lastname: Viswanathan
authors.forename: L Jackson
authors.initials: LJ
authors.lastname: Roberts
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Balser
mesh_terms: D000447:Aldehydes / Q000494:pharmacology; D001145:Arrhythmias, Cardiac / Q000209:etiology*; D002460:Cell Line; D005424:Flecainide / Q000494:pharmacology; D006801:Humans; D028421:Isoprostanes / Q000378:metabolism; D015227:Lipid Peroxidation; D009203:Myocardial Infarction / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D018384:Oxidative Stress; D015222:Sodium Channels / Q000502:physiology*; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D000447:Aldehydes; D028421:Isoprostanes; D015222:Sodium Channels; D020122:tert-Butylhydroperoxide; D005424:Flecainide