Polarimetric imaging of subsurface tissue-numerical results.
Tissue images obtained at deeper depths lack significant contrast. To enhance the contrast of these images and to increase the visibility of subsurface tissues, a method is proposed. This technique is based on the principle that photons at longer wavelengths penetrate deeper than photons at shorter wavelengths. In this technique, images in the original and orthogonal polarized states are recorded with the source illumination in linear polarized state at two different wavelengths. Image subtraction of a fraction of the copolarized image from the perpendicular polarized state is done on these images. The images obtained after the first processing are subjected to the next image subtraction where the shorter wavelength image is subtracted from the longer wavelength image. Monte Carlo simulations show that the resultant image has marked contrast up to 2.5 cm.
Sudha
Gnanou F
GF
Pondicherry Engineering College, Pondicherry 605 014, India. gfsudha@rediffmail.com
Palanivelu
Theerta G
TG
D000465:Algorithms; D000818:Animals; D003198:Computer Simulation; D003238:Connective Tissue / Q000648:ultrastructure*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008859:Microscopy, Polarization / Q000379:methods*; D008954:Models, Biological; D009010:Monte Carlo Method; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000379:methods*