Miniaturized thermal lens and fluorescence detection system for microchemical chips.
We have developed a miniaturized two-way detection system using thermal lens and fluorescence spectroscopies for microchip chemistry. The system was composed of laser diode (LD) modules, fiber-based optics combined with a gradient index lens, and miniaturized detection units for thermal lens and fluorescence signals. The detection limits in the thermal lens and fluorescence spectroscopies were 6.3 x 10(-9)M for Ni(II) phthalocyanine tetrasulfonic acid and 3.0 x 10(-9)M for cy5, respectively. The performance of the system with the miniaturized thermal lens was equivalent to that of a conventional thermal lens microscope. The fluorescence sensitivity was comparable to sensitivities offered by conventional miniaturized systems.
Yamauchi
Masayo
M
Institute of Microchemical Technology, 3-2-1 Sakado, Takatsu, Kawasaki 213-0012, Japan.
Tokeshi
Manabu
M
Yamaguchi
Jun
J
Fukuzawa
Takashi
T
Hattori
Akihiko
A
Hibara
Akihide
A
Kitamori
Takehiko
T
D008904:Miniaturization; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature