Glial membranes at the node of Ranvier prevent neurite outgrowth.
Nodes of Ranvier are regularly placed, nonmyelinated axon segments along myelinated nerves. Here we show that nodal membranes isolated from the central nervous system (CNS) of mammals restricted neurite outgrowth of cultured neurons. Proteomic analysis of these membranes revealed several inhibitors of neurite outgrowth, including the oligodendrocyte myelin glycoprotein (OMgp). In rat spinal cord, OMgp was not localized to compact myelin, as previously thought, but to oligodendroglia-like cells, whose processes converge to form a ring that completely encircles the nodes. In OMgp-null mice, CNS nodes were abnormally wide and collateral sprouting was observed. Nodal ensheathment in the CNS may stabilize the node and prevent axonal sprouting.
Huang
Jeffrey K
JK
Fishberg Department of Neuroscience, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA.
Phillips
Greg R
GR
Roth
Alejandro D
AD
Pedraza
Liliana
L
Shan
Weisong
W
Belkaid
Wiam
W
Mi
Sha
S
Fex-Svenningsen
Asa
A
Florens
Laurence
L
Yates
John R
JR
Colman
David R
DR
D000818:Animals; D000941:Antigens / Q000032:analysis; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D002417:Cattle; D022081:Cell Surface Extensions / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D002478:Cells, Cultured; D058851:GPI-Linked Proteins; D005727:Ganglia, Spinal / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D051379:Mice; D009185:Myelin Proteins; D009186:Myelin Sheath / Q000737:chemistry; D018992:Myelin-Associated Glycoprotein / Q000032:analysis; D063308:Myelin-Oligodendrocyte Glycoprotein; D016501:Neurites / Q000502:physiology* / Q000648:ultrastructure; D009457:Neuroglia / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure*; D009836:Oligodendroglia / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D011509:Proteoglycans / Q000032:analysis; D040901:Proteomics; D011901:Ranvier's Nodes / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D013116:Spinal Cord / Q000166:cytology; D000099216:Chondroitin Sulfate Proteoglycan 4
D000941:Antigens; D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4; C571326:MOG protein, human; C571327:Mog protein, mouse; C571328:Mog protein, rat; C560528:OMG protein, human; C560529:Omg protein, mouse; C560532:Omg protein, rat