Clonal amniotic fluid-derived stem cells express characteristics of both mesenchymal and neural stem cells.
Recent evidence has shown that amniotic fluid may be a novel source of fetal stem cells for therapeutic transplantation. We previously developed a two-stage culture protocol to isolate a population of amniotic fluid-derived mesenchymal stem cells (AFMSCs) from second-trimester amniocentesis. AFMSCs maintain the capacity to differentiate into multiple mesenchymal lineages and neuron-like cells. It is unclear whether amniotic fluid contains heterogeneous populations of stem cells or a subpopulation of primitive stem cells that are similar to marrow stromal cells showing the behavior of neural progenitors. In this study, we showed a subpopulation of amniotic fluid-derived stem cells (AF-SCs) at the single-cell level by limiting dilution. We found that NANOG- and POU5F1 (also known as OCT4)-expressing cells still existed in the expanded single cell-derived AF-SCs. Aside from the common mesenchymal characteristics, these clonal AF-SCs also exhibit multiple phenotypes of neural-derived cells such as NES, TUBB3, NEFH, NEUNA60, GALC, and GFAP expressions both before and after neural induction. Most importantly, HPLC analysis showed the evidence of dopamine release in the extract of dopaminergic-induced clonal AF-SCs. The results of this study suggest that besides being an easily accessible and expandable source of fetal stem cells, amniotic fluid will provide a promising source of neural progenitor cells that may be used in future cellular therapies for neurodegenerative diseases and nervous system injuries.
Tsai
Ming-Song
MS
Prenatal Diagnosis Center, Cathay General Hospital, Taipei 106, Taiwan. mstsai@cgh.org.tw
Hwang
Shiaw-Min
SM
Tsai
Yieh-Loong
YL
Cheng
Fu-Chou
FC
Lee
Jia-Ling
JL
Chang
Yu-Jen
YJ
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology; D000653:Amniotic Fluid / Q000166:cytology*; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D002999:Clone Cells; D004298:Dopamine / Q000378:metabolism; D053686:Fetal Stem Cells / Q000166:cytology* / Q000502:physiology; D005434:Flow Cytometry; D006367:HeLa Cells; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D000071317:Nanog Homeobox Protein; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D010011:Osteocytes / Q000166:cytology
D015415:Biomarkers; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D015102:3,4-Dihydroxyphenylacetic Acid; D004298:Dopamine