Single microspore reverse transcription-polymerase chain reaction: dynamic change of gene expression during microsporogenesis.
A sensitive and reliable single microspore reverse transcription-polymerase chain reaction (RT-PCR) assay was developed that requires little sample material and only a short sampling time and ensures template preservation. We propose that the assay provides a useful tool to study not only cell-cycle related events in developing pollen but also the molecular mechanisms underlying male sterility and mutants impaired in pollen development.
Zhou
Bing
B
Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, P. R. China.
Sauter
Margret
M
Sun
Mengxiang
M
D002455:Cell Division / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008938:Mitosis / Q000235:genetics*; D012275:Oryza / Q000166:cytology / Q000235:genetics; D059828:Plant Cells; D011058:Pollen / Q000235:genetics* / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction