title: Synthetic, site-specific biotinylated analogs of human MCP-1.
abstract: Human monocyte chemoattractant protein 1 (MCP-1, CCL2) is a 8.6-kDa protein that has been implicated in a number of diseases including atherosclerosis, rheumatoid arthritis, chronic obstructive pulmonary disease and cancer. As part of a program to identify antibodies against MCP-1, we synthesized site-specific, biotinylated human MCP-1 analogs to be used for panning of an antibody phage display library. In contrast to material obtained from random biotinylation, the site-specific biotinylated analogs were homogeneous and retained full activity.
authors.affiliation: Centocor R&D, Inc., 145 King of Prussia Road, Radnor, PA 19087, USA. mkruszy2@cntus.jnj.com
authors.forename: Marian
authors.initials: M
authors.lastname: Kruszynski
authors.forename: Ping
authors.initials: P
authors.lastname: Tsui
authors.forename: Nicole
authors.initials: N
authors.lastname: Stowell
authors.forename: Jinquan
authors.initials: J
authors.lastname: Luo
authors.forename: Jennifer F
authors.initials: JF
authors.lastname: Nemeth
authors.forename: Anuk M
authors.initials: AM
authors.lastname: Das
authors.forename: Raymond
authors.initials: R
authors.lastname: Sweet
authors.forename: George A
authors.initials: GA
authors.lastname: Heavner
mesh_terms: D000595:Amino Acid Sequence; D019921:Biotinylation; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D018932:Chemokine CCL2 / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018932:Chemokine CCL2; D002118:Calcium