A CCR5-tropic simian-HIV molecular clone capable of inducing AIDS in rhesus macaques.
We previously reported the derivation of a CCR5 (R5)-tropic pathogenic strain SHIVSF162P3. Here, we show that a simian-HIV (SHIV) molecular clone expressing the entire env gp160 of SHIVSF162P3, termed SHIV P3gp160, could fully recapitulate the in vivo replicative characteristics of the parental isolate. SHIV P3gp160 is mucosally transmissible, preferentially depletes memory CD4 T cells, and induced simian AIDS in 2 of 6 infected macaques. The availability of an infectious R5 SHIV molecular clone that can be transmitted mucosally and causes disease provides an important reagent for studies of lentiviral pathogenesis and AIDS vaccine research.
Hsu
Mayla
M
Aaron Diamond AIDS Research Center, Rockefeller University, New York, NY 10016, USA.
Ho
Siu-Hong
SH
Balfe
Peter
P
Gettie
Agegnehu
A
Harouse
Janet
J
Blanchard
James
J
Cheng-Mayer
Cecilia
C
D000163:Acquired Immunodeficiency Syndrome / Q000503:physiopathology / Q000635:transmission / Q000821:virology*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D018791:CD4 Lymphocyte Count; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D005434:Flow Cytometry; D015686:Gene Products, env / Q000235:genetics; D006678:HIV / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D019302:HIV Envelope Protein gp160 / Q000235:genetics; D016131:Lymphocyte Subsets; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000097:blood; D019713:Receptors, CCR5 / Q000378:metabolism*; D011995:Recombination, Genetic; D016097:Simian Acquired Immunodeficiency Syndrome / Q000503:physiopathology / Q000635:transmission / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D019562:Viral Load
D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D012367:RNA, Viral; D019713:Receptors, CCR5; C087575:SIV envelope glycoprotein gp160