[The effect of sodium doecyl sulfate on Streptococcus sanguis biofilm].
OBJECTIVE
To investigate the effects of sodium doecyl sulfate on Streptococcus sanguis biofilm.


METHODS
Streptococcus sanguis biofilm was formed on saliva-coated glass(SCG) in a flow culture system, then exposed to different dental plaque control agents for 3,10,30 minutes including A: 300 mmol.L(-1) sodium doecyl sulfate (SDS); B: 2% chlorhexidine combined with 300 mmol.L(-1) SDS; C: 0.3%triclosan combined with 300 mmol.L(-1) SDS. Confocal laser scanning microscopy and vital/dead fluorescent staining technique (vital stained green, dead stained red) were combined to observe biofilm thickness, bacterial density and viability, analysis of variance was used for comparison.


RESULTS
The biofilm thickness and bacteria density reduced after treatment by 300 mmol.L(-1) SDS. However, there was no significant difference after treatment by 2% chlorhexidine combined with 300 mmol.L(-1)SDS; When treated by 0.3% triclosan combined with 300 mmol.L(-1) SDS, most or all of the bacteria detached from the surface and dispersed.


CONCLUSIONS
The combination of SDS (aimed at detachment) and triclosan (aimed at killing bacteria) may be most effective in controlling biofilm.
Ma
Rui
R
Department of Oral Medicine, School of Stomatology, Ninth People's Hospital, Shanghai Jiaotong University, China. marui6868@yahoo.com
Zhu
Min
M
Liu
Zheng
Z
D018441:Biofilms; D002710:Chlorhexidine; D003773:Dental Plaque; D012463:Saliva; D012967:Sodium Dodecyl Sulfate; D013298:Streptococcus sanguis; D014260:Triclosan
D012967:Sodium Dodecyl Sulfate; D014260:Triclosan; D002710:Chlorhexidine