A point mutation in the NFYC gene generates an antigenic peptide recognized by autologous cytolytic T lymphocytes on a human squamous cell lung carcinoma.
We have identified an antigen recognized by cytolytic T lymphocytes (CTL) on the autologous tumor cells of a nonsmall cell lung cancer patient. The antigenic peptide, presented by HLA-B*5201 molecules, was encoded by a mutated sequence in the gene coding for the C subunit of transcription factor NF-Y. The mutation was present in the tumor sample from which the cell line was derived, and appeared to be unique to the tumor of this patient. In a lymph node draining the tumor, precursors of CTL recognizing the autologous tumor cells were detected at a frequency of about 1/30,000 of the CD8 cells, and 85% of them recognized the mutated NF-YC peptide, suggesting that the patient mounted a T cell response against this antigen. These results strengthened the notion that unique tumor-specific antigens are highly represented not only in melanoma but also in other types of tumors, like nonsmall cell lung cancer.
Takenoyama
Mitsuhiro
M
Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
Baurain
Jean-Françis
JF
Yasuda
Manabu
M
So
Tomoko
T
Sugaya
Masakazu
M
Hanagiri
Takeshi
T
Sugio
Kenji
K
Yasumoto
Kosei
K
Boon
Thierry
T
Coulie
Pierre G
PG
D000951:Antigens, Neoplasm; D023081:CCAAT-Binding Factor / Q000235:genetics* / Q000276:immunology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000276:immunology; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D023081:CCAAT-Binding Factor; D004268:DNA-Binding Proteins; C469358:NFYC protein, human; D014157:Transcription Factors