[Effects of intra-arterial perfusion of cisplatin combined with concomitant radiation therapy on the healing of microvascular anastomoses: an experimental study in rabbits].
OBJECTIVE
To investigate the effects of intra-arterial perfusion of cisplatin and concomitant radiation therapy on the microvascular structure and the healing of anastomoses.


METHODS
Three different treatments including intra-arterial perfusion of saline, intra-arterial perfusion of cisplatin, intra-arterial perfusion of saline and 25 Gy radiation therapy as well as intra-arterial perfusion of cisplatin and 25 Gy radiation therapy were conducted in 40 femoral arteries of 20 rabbits, which divided into 4 groups, control group, intra-arterial chemotherapy group, radiation therapy group and combined treatment group accordingly. The bilateral femoral arteries were transected and anastomosed using microsurgical technique on 7th day after the treatments were completed. The effects of different treatments on the microvascular structure and the patency rates of anastomoses were investigated.


RESULTS
The evidences of chronic damage to the vascular wall were observed in 4 groups. The changes in the combined treatment group were most serious. The damage to arterial wall in the radiation therapy group, chemotherapy group, and control group decrease gradually in order. Although the damages of the vascular wall were observed, there were no differences in the patency rates after microvascular anastomoses.


CONCLUSIONS
These vessels can be used with caution as recipient vessels for free tissue transfer.
Zhang
Shi-zhou
SZ
Department of Oral and Maxillogicial Surgery, Shandong Provincial Hospital, Shangdong University, Jinan 250021, China.
Wang
Zhong-He
ZH
Hu
Hai-sheng
HS
D000818:Animals; D001163:Arteriovenous Anastomosis; D002945:Cisplatin / Q000009:adverse effects* / Q000627:therapeutic use*; D010477:Perfusion; D011817:Rabbits; D011878:Radiotherapy / Q000009:adverse effects*; D014945:Wound Healing
D002945:Cisplatin