title: Proteome analysis of human amnion and amniotic fluid by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: Proteome analysis by 2-DE and PMF by MALDI-TOF MS was performed on human amnion and amniotic fluid at term. Ninety-two soluble and nineteen membrane proteins were identified from amnion. Thirty-five proteins were identified from amniotic fluid. Calgranulin A and B were found in all patients infected with Ureaplasma urealyticum, but not in any of the patients without infection, indicating that they are potential markers of intrauterine infection. Identity of calgranulin A and B was confirmed by MALDI-TOF/TOF MS. This study represents the first extensive analysis of the human amnion and amniotic fluid proteome at term and demonstrates that 2-DE and MALDI-TOF MS is a useful tool for identifying clinically significant biomarkers of problematic pregnancies.
authors.affiliation: Department of Chemistry, Seoul National University, Gwanak-gu, Seoul, Korea 151-747.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Park
authors.forename: Won-Gap
authors.initials: WG
authors.lastname: Yoon
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Song
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Jung
authors.forename: Chong Jai
authors.initials: CJ
authors.lastname: Kim
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Oh
authors.forename: Bo Hyun
authors.initials: BH
authors.lastname: Yoon
authors.forename: Guhung
authors.initials: G
authors.lastname: Jung
authors.forename: Hie-Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Takashi
authors.initials: T
authors.lastname: Nirasawa
mesh_terms: D000595:Amino Acid Sequence; D000650:Amnion / Q000737:chemistry*; D000653:Amniotic Fluid / Q000737:chemistry*; D001483:Base Sequence; D040501:Calgranulin A / Q000302:isolation & purification; D040502:Calgranulin B / Q000737:chemistry / Q000302:isolation & purification; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D016869:Ureaplasma Infections / Q000378:metabolism
chemical_list: D040501:Calgranulin A; D040502:Calgranulin B; D017931:DNA Primers; D020543:Proteome