title: Gene cloning and properties of the RND-type multidrug efflux pumps MexPQ-OpmE and MexMN-OprM from Pseudomonas aeruginosa.
abstract: We cloned two operons for putative RND-type multidrug efflux pumps from Pseudomonas aeruginosa by a PCR method. We designated the genes in one operon mexPQ(-opmE) and in another operon mexMN. Introduction of the mexPQ-opmE into drug hypersensitive cells resulted in elevated MICs of macrolides, fluoroquinolones and some other drugs. Introduction of the mexMN into the hypersensitive cells possessing oprM, but not into cells not possessing oprM, resulted in elevated MICs of chloramphenicol and thiamphenicol. Thus, we conclude that MexPQ-OpmE and MexMN-OprM are functional multidrug efflux pumps when expressed in P. aeruginosa.
authors.affiliation: Department of Molecular Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Mima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sekiya
authors.forename: Tohru
authors.initials: T
authors.lastname: Mizushima
authors.forename: Teruo
authors.initials: T
authors.lastname: Kuroda
authors.forename: Tomofusa
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018432:Drug Resistance, Multiple / Q000235:genetics* / Q000502:physiology; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D009876:Operon; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins