Effects of oxidative compounds on thermotolerance in escherichia coli O157:H7 strains EO139 and 380-94.
An oxidative complex composed of ferric iron chloride hexahydrate, ADP, and ascorbic acid can generate hydrogen peroxide and hydroxyl radicals in fibroblasts. These compounds are naturally found in meat and meat-based products and may elicit oxidative stress on Escherichia coli O157:H7, thus conferring thermotolerance to the bacterium due to the phenomenon of the global stress response. The effect of the levels of the oxidative complex on the thermotolerance of E. coli O157:H7 was investigated. Cultures of E. coli O157:H7 strains EO139 and 380-94 were mixed in three different concentrations (10:10: 40, 15:15:60, and 20:20:80 microM) of the oxidative complex (iron III chloride, ADP, and ascorbic acid, respectively). The samples were inserted into capillary tubes and heated in a circulating water bath at 59 and 60 degrees C for EO139 and 380-94, respectively. Tubes were removed at intervals of 5 min for up to 1 h and contents spirally plated on plate count agar that was incubated for 48 h at 37 degrees C. The thermotolerance of both E. coli O157:H7 strains EO139 and 380-94 was influenced by the concentrations of the oxidative complex. The ratio of 10:10:40 microM enhanced thermotolerance of EO139 and 390-94 at 59 and 60 degrees C, respectively. However, exposure to the ratios of 15:15:60 and 20:20:80 microM rendered the pathogen more sensitive to the lethal effect and did not enhance the thermotolerance of the cells. The significance of this study is twofold. This experiment proves that oxidative stress can enhance thermotolerance of bacterial cells induced by an oxidative complex if only in a specific ratio and concentration. It is possible to speculate that if the chemical compounds are present in this ratio in meats, they may enhance the thermal resistance of E. coli O157:H7 and make the bacteria more difficult to eliminate, thus increasing the risk of foodborne illness in consumers.
Blackman
Isabel C
IC
Agricultural Research Station, Fort Valley State University, Fort Valley, Georgia 31030-4313, USA. blackmani@fvsu.edu
Park
Young W
YW
Harrison
Mark A
MA
D000222:Adaptation, Physiological; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D003470:Culture Media; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development*; D005516:Food Microbiology; D006358:Hot Temperature; D058085:Iron Compounds / Q000494:pharmacology; D008460:Meat / Q000382:microbiology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D013997:Time Factors
D000975:Antioxidants; D003470:Culture Media; D058085:Iron Compounds; D000244:Adenosine Diphosphate; D001205:Ascorbic Acid