title: New hyphenated methodologies in high-sensitivity glycoprotein analysis.
abstract: High-sensitivity glycoprotein analyses are of particular interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of new hyphenated methodologies in high-sensitivity glycoprotein analysis is highlighted in this thematic review. These methodologies include, in particular, capillary LC/MALDI/TOF/TOF MS in conjunction with online permethylation platform, and silica-based lectin microcolumns interfaced to MS. The potential of these methodologies in glycomic and glycoproteomic analysis is demonstrated for model glycoproteins as well as total glycomes and glycoproteomes derived from biological samples. Additionally, the applications of CE-MS, CEC, and nanoLC with graphitized carbon in the areas of glycomics and glycoproteomics are described.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington 47405, USA. novotny@indiana.edu
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
authors.forename: Yehia
authors.initials: Y
authors.lastname: Mechref
mesh_terms: D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D006023:Glycoproteins / Q000032:analysis*; D037102:Lectins; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006023:Glycoproteins; D037102:Lectins