title: Coordinate control of cell cycle regulatory genes in zebrafish development tested by cyclin D1 knockdown with morpholino phosphorodiamidates and hydroxyprolyl-phosphono peptide nucleic acids.
abstract: During early zebrafish (Danio rerio) development zygotic transcription does not begin until the mid-blastula transition (MBT) 3 h after fertilization. MBT demarcates transition from synchronous short cell cycles of S and M phases exclusively to full cycles encompassing G1 and G2 phases. Transcriptional profiling and RT-PCR analyses during these phases enabled us to determine that this shift corresponds to decreased transcript levels of S/M phase cell cycle control genes (e.g. ccna2, ccnb1, ccnb2 and ccne) and increased transcript levels of ccnd1, encoding cyclin D1, and orthologs of p21 (p21-like) and retinoblastoma (Rb-like 1). To investigate the regulation of this process further, the translation of ccnd1 mRNA, a G1/S checkpoint control element, was impaired by microinjection of ccnd1-specific morpholino phosphorodiamidate (MO) 20mer or hydroxyprolyl-phosphono peptide nucleic acid (HypNA-pPNA) 16mer antisense oligonucleotides. The resulting downregulation of cyclin D1 protein resulted in microophthalmia and microcephaly, but not lethality. The phenotypes were not seen with 3-mismatch MO 20mers or 1-mismatch HypNA-pPNA 16mers, and were rescued by an exogenous ccnd1 mRNA construct with five mismatches. Collectively, these results indicate that transcription of key molecular determinants of asynchronous cell cycle control in zebrafish embryos commences at MBT and that the reduction of cyclin D1 expression compromises zebrafish eye and head development.
authors.affiliation: Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA, USA.
authors.forename: Kevin T
authors.initials: KT
authors.lastname: Duffy
authors.forename: Mary Frances
authors.initials: MF
authors.lastname: McAleer
authors.forename: William R
authors.initials: WR
authors.lastname: Davidson
authors.forename: Laszlo
authors.initials: L
authors.lastname: Kari
authors.forename: Csaba
authors.initials: C
authors.lastname: Kari
authors.forename: Chang-Gong
authors.initials: CG
authors.lastname: Liu
authors.forename: Steven A
authors.initials: SA
authors.lastname: Farber
authors.forename: Keith C
authors.initials: KC
authors.lastname: Cheng
authors.forename: Jason R
authors.initials: JR
authors.lastname: Mest
authors.forename: Eric
authors.initials: E
authors.lastname: Wickstrom
authors.forename: Adam P
authors.initials: AP
authors.lastname: Dicker
authors.forename: Ulrich
authors.initials: U
authors.lastname: Rodeck
mesh_terms: D000818:Animals; D019938:Cyclin D1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology; D015536:Down-Regulation; D005123:Eye / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018816:Genes, cdc; D060172:Morpholinos; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology*; D020135:Peptide Nucleic Acids / Q000737:chemistry; D014158:Transcription, Genetic; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D060172:Morpholinos; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D020135:Peptide Nucleic Acids; C505966:hydroxyprolyl-phospho peptide nucleic acid antisense oligonucleotide; D019938:Cyclin D1