NO signaling in ARE-mediated gene expression.
Nitric oxide (NO) and peroxynitrite, which serve as cell signal molecules, activate the antioxidant response element (ARE) for the induction of phase II antioxidant enzymes as an adaptive response. The reactive nitrogen species plays an essential role in Nrf2 activation and Nrf2 binding to the ARE present in the target genes. In this chapter, we describe the system by which the NO signaling pathway regulates ARE-mediated gene expression, which includes immunochemical assessment and gel shift analysis of Nrf2 activation.
Park
Eun Young
EY
College of Pharmacy, Seoul National University, Kwanak-gu, Seoul, South Korea.
Kim
Sang Geon
SG
D000818:Animals; D000975:Antioxidants; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D015342:DNA Probes; D005786:Gene Expression Regulation; D006801:Humans; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D018384:Oxidative Stress; D015398:Signal Transduction
D000975:Antioxidants; D015342:DNA Probes; D051267:NF-E2-Related Factor 2; D009569:Nitric Oxide