title: A role for the neuronal protein collapsin response mediator protein 2 in T lymphocyte polarization and migration.
abstract: The semaphorin-signaling transducer collapsin response mediator protein 2 (CRMP2) has been identified in the nervous system where it mediates Sema3A-induced growth cone navigation. In the present study, we provide first evidence that CRMP2 is present in the immune system and plays a critical role in T lymphocyte function. CRMP2 redistribution at the uropod in polarized T cells, a structural support of lymphocyte motility, suggests that it may regulate T cell migration. This was evidenced in primary T cells by small-interfering RNA-mediated CRMP2 gene silencing and blocking Ab, as well as CRMP2 overexpression in Jurkat T cells tested in a chemokine- and semaphorin-mediated transmigration assay. Expression analysis in PBMC from healthy donors showed that CRMP2 is enhanced in cell subsets bearing the activation markers CD69+ and HLA-DR+. Heightened expression in T lymphocytes of patients suffering from neuroinflammatory disease with enhanced T cell-transmigrating activity points to a role for CRMP2 in pathogenesis. The elucidation of the signals and mechanisms that control this pathway will lead to a better understanding of T cell trafficking in physiological and pathological situations.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 433 and Institut Fédératif de Recherche 19, Faculté de Médecine R. Laënnec, Lyon, France.
authors.forename: Peggy
authors.initials: P
authors.lastname: Vincent
authors.forename: Yves
authors.initials: Y
authors.lastname: Collette
authors.forename: Romain
authors.initials: R
authors.lastname: Marignier
authors.forename: Carine
authors.initials: C
authors.lastname: Vuaillat
authors.forename: Véronique
authors.initials: V
authors.lastname: Rogemond
authors.forename: Nathalie
authors.initials: N
authors.lastname: Davoust
authors.forename: Christophe
authors.initials: C
authors.lastname: Malcus
authors.forename: Sylvie
authors.initials: S
authors.lastname: Cavagna
authors.forename: Antoine
authors.initials: A
authors.lastname: Gessain
authors.forename: Irma
authors.initials: I
authors.lastname: Machuca-Gayet
authors.forename: Marie-Françoise
authors.initials: MF
authors.lastname: Belin
authors.forename: Tam
authors.initials: T
authors.lastname: Quach
authors.forename: Pascale
authors.initials: P
authors.lastname: Giraudon
mesh_terms: D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D002465:Cell Movement / Q000276:immunology*; D005434:Flow Cytometry; D020868:Gene Silencing; D006684:HLA-DR Antigens / Q000276:immunology / Q000378:metabolism; D015490:HTLV-I Infections / Q000276:immunology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D019169:Jurkat Cells; D037181:Lectins, C-Type; D009419:Nerve Tissue Proteins; D011506:Proteins / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D014162:Transfection; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D006684:HLA-DR Antigens; D036341:Intercellular Signaling Peptides and Proteins; D037181:Lectins, C-Type; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger; D000099192:CD69 Antigens; C095114:collapsin response mediator protein-2