Hypertonic saline and hemorrhagic shock: hepatocellular function and integrity after six hours of treatment.
OBJECTIVE
The comparison after 6 h of hemorrhagic shock (HS) treatment with NaCl 7.5% (Hypertonic Saline Solution-SSH) or Ringer Lactate (RL) on liver function and integrity.


METHODS
Male Wistar rats were submitted to HS (Mean Arterial Pressure-MAP= 45 mmHg) during 60 min and then treated with NaCl 7.5% (SSH, 10% of blood loss, n=8) or Ringer Lactate (RL, 400% of blood loss, n=8). After 6 h rats were anesthetized, hepatic function was assessed by bile flow measurement and liver integrity evaluated by determination of alanine aminotransferase (ALT) and bilirubin activities.


RESULTS
There was no difference in MAP between the groups during the whole experiments. Biliary flow showed a significant recovery after SSH treatment (p<0.05), and significant decrease of ALT (p<0.001) and bilirubin levels (p<0.001) in comparison to RL.


CONCLUSIONS
Resuscitation of HS with NaCl 7.5% promoted better recovery of liver function and lesser hepatocellular damage after 6 h of treatment compared to RL. The improvement is very likely related to increased microvascular perfusion provided by small volume resuscitation.
Hoppen
Ricardo Antônio
RA
UFRGS, Porto Alegre, Brazil. rhoppen@annex.com.br
Corso
Carlos Otávio
CO
Grezzana
Tomáz Jesus Maria
TJ
Severino
Antonio
A
Dal-Pizzol
Felipe
F
Ritter
Cristiane
C
D000410:Alanine Transaminase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001663:Bilirubin / Q000097:blood; D004195:Disease Models, Animal; D007552:Isotonic Solutions / Q000627:therapeutic use*; D008099:Liver / Q000502:physiology* / Q000503:physiopathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012151:Resuscitation / Q000379:methods*; D000077325:Ringer's Lactate; D012462:Saline Solution, Hypertonic / Q000627:therapeutic use*; D012771:Shock, Hemorrhagic / Q000628:therapy*; D018709:Statistics, Nonparametric
D007552:Isotonic Solutions; D000077325:Ringer's Lactate; D012462:Saline Solution, Hypertonic; D000410:Alanine Transaminase; D001663:Bilirubin