Effects of soluble ferri-hydroxide complexes on microbial neutralization of acid mine drainage.
Heterotrophic respiration of ferric iron by Acidiphilium cryptum was investigated in anoxic microcosms with initial media pH values from 1.5 to 3.5. No organic carbon consumption or iron reduction was observed with an initial pH of 1.5, indicating that A. cryptum may not be capable of iron respiration at this pH. Significant iron reduction was observed at pH 2.5 and 3.5, with different effects. When the initial pH was 3.5, pH increased to 4.7-5.5 over 60 days of incubation with simultaneous production of 0.4 g L(-1) Fe2+. However, at an initial pH of 2.5, no significant change in pH was observed during iron respiration, although the accumulation of soluble ferrous iron was significantly higher, averaging 1.1 g L(-1) Fe2+. The speciation of the ferric iron electron acceptor may explain these results. At pH values of 3.5 and higher, precipitated ferric hydroxide Fe- (OH)3 would have been the primary source of ferric iron, with reduction resulting in net production of OH- ions and the significant increases in media pH observed. However at pH 2.5, soluble complexes, FeOH2+ and Fe(OH)2+, may have been the more prevalent electron acceptors, and the alkalinity generated by reduction of complexed iron was low. The existence of charged ferri-hydroxide complexes at pH 2.5 was verified by voltammetry. Results suggest that initiation of bacterial iron reduction may result in neutralization of acid mine drainage. However, this effect is extremely sensitive to iron speciation within a relatively small and critical pH range.
Bilgin
A Azra
AA
Department of Civil, Environmental, and Architectural Engineering, University of Colorado, Boulder, Colorado 80309, USA. abilgin@caltech.edu
Silverstein
JoAnn
J
Hernandez
Mark
M
D041801:Acidiphilium / Q000378:metabolism*; D004563:Electrochemistry; D005290:Ferric Compounds / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008906:Mining; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D005290:Ferric Compounds; D014874:Water Pollutants, Chemical; C021024:ferric hydroxide