Paenibacillus mendelii sp. nov., from surface-sterilized seeds of Pisum sativum L.
A Gram-variable, facultatively anaerobic, endospore-forming bacterium was isolated from surface-sterilized seeds of the garden pea and characterized with phenotypic and molecular methods. A PCR with the Paenibacillus-specific primer PAEN515F and the 16S rRNA gene sequence indicated that strain C/2T belongs to the genus Paenibacillus and is closely related to Paenibacillus phyllosphaerae (94.0 % sequence similarity). Strain C/2T generated a unique phenotypic profile, in particular for the production of acid from substrates. The DNA G+C content (50.8 mol%) and the major fatty acid (anteiso-C(15 : 0)) are consistent with the genus Paenibacillus. DNA-DNA hybridization distinguished strain C/2T from other phylogenetically related Paenibacillus species and, therefore, strain C/2T (=CCM 4839T=LMG 23002T) is here described as the type strain of a novel species, for which the name Paenibacillus mendelii sp. nov. is proposed.
Šmerda
Jakub
J
Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, 613 00 Brno, Czech Republic.
Sedláček
Ivo
I
Czech Collection of Microorganisms, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
Páčová
Zdena
Z
Czech Collection of Microorganisms, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
Durnová
Eva
E
Institute of Public Health in Ostrava, Partyzánské nám. 7, 702 00 Ostrava, Czech Republic.
Smíšková
Alexandra
A
Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, 613 00 Brno, Czech Republic.
Havel
Ladislav
L
Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, 613 00 Brno, Czech Republic.
D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D018532:Pisum sativum / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D012639:Seeds / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S