title: Antibodies against human Toll-like receptors (TLRs): TLR distribution and localization in human dendritic cells.
abstract: We have produced monoclonal antibodies (mAbs) against human Toll-like receptors (TLRs). These mAbs recognize the natural conformation of the extracellular domain leucine-rich repeats and, thereby, are suitable for immunoprecipitation, flow cytometric analysis and immunostaining. Using these mAbs, we determined the distribution of TLRs in a variety of human cell populations. Human TLRs that recognize bacterial components, particularly TLR-1, TLR-2, TLR-4 and TLR-6, reside on the cell surface and are expressed in myeloid and monocyte-derived dendritic cells (DCs) but not in plasmacytoid DCs (pDCs). Human TLR-3 resides in putative endosomes in myeloid DCs. Thus, the human myeloid DC subset harbors a unique and distinctive TLR repertoire. These mAbs will be useful to test the localization and distribution of human TLRs in a variety of cells and organs. Functional studies of human TLRs will also be feasible since certain of them are function-blocking mAbs.
authors.affiliation: Department of Microbiology and Immunology, Hokkaido University Graduate School of Medicine, Sapporo, Japan. seya-tu@med.hokudai.ac.jp
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Seya
authors.forename: Kenji
authors.initials: K
authors.lastname: Funami
authors.forename: Mitsue
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Misako
authors.initials: M
authors.lastname: Matsumoto
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D051193:Toll-Like Receptors / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D051193:Toll-Like Receptors