title: Sustained gastric mucosal acidosis after hemorrhage in spite of rapid hemodynamic restoration with blood or hypertonic/hyperoncotic solution.
abstract: Splanchnic hypoperfusion has been implicated as the motor of multiple organ dysfunction. Hypertonic saline has shown to benefit microcirculatory blood flow. In hemorrhaged animals, we tested the hypothesis that small-volume 3% NaCl/10% dextran 40 (3%HSD) promotes global and regional improvements, including gastric mucosal acidosis reversal. Seventeen dogs (18.8 +/- 1.2 kg) were bled (20 mL/min) to a mean arterial pressure of 40-45 mm Hg, which was maintained at these levels for 15 min. They were randomly assigned to two groups: Blood (n = 9), total shed blood retransfused at 40 mL/min; or a 4-min bolus injection of 3%HSD (n = 8), in a volume equivalent to 25% of total shed blood. All animals were followed for 30 min thereafter. Gastric mucosal PCO2 (gas tonometry), portal vein PCO2, superior mesenteric artery blood flow (SMA, ultrasonic flowprobes), and systemic and regional O2-derived variables were evaluated throughout the protocol. Hemorrhage induced significant reductions of arterial pressure, cardiac output, and SMA blood flow, while portal-arterial and gastric-arterial PCO2 gradients increased. Total shed blood transfusion, as well as 3%HSD bolus injection, promptly restored all parameters, except for the increased gastric-arterial PCO2 gradient. We conclude that persistent gastric mucosal acidosis cannot be adequately predicted by global and splanchnic O2 derived variables in following hemorrhage and resuscitation with total shed blood transfusion or small-volume hypertonic-hyperoncotic solution.
authors.affiliation: Research Division, Heart Institute (InCor) and LIM11, University of São Paulo Medical School, São Paulo, SP, Brazil. expluiz@incor.usp.br
authors.forename: Luiz Francisco
authors.initials: LF
authors.lastname: Poli de Figueiredo
authors.forename: Ruy Jorge
authors.initials: RJ
authors.lastname: Cruz
authors.forename: Eliezer
authors.initials: E
authors.lastname: Silva
authors.forename: Margareth Macae
authors.initials: MM
authors.lastname: Yada-Langui
authors.forename: Mauricio
authors.initials: M
authors.lastname: Rocha e Silva
mesh_terms: D000138:Acidosis / Q000209:etiology*; D000818:Animals; D001794:Blood Pressure; D001803:Blood Transfusion; D001810:Blood Volume; D002245:Carbon Dioxide / Q000097:blood; D002302:Cardiac Output; D003911:Dextrans / Q000008:administration & dosage*; D004285:Dogs; D005440:Fluid Therapy / Q000379:methods*; D005753:Gastric Mucosa / Q000378:metabolism*; D006470:Hemorrhage / Q000378:metabolism* / Q000628:therapy; D008297:Male; D012151:Resuscitation; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage*; D013152:Splanchnic Circulation
chemical_list: D003911:Dextrans; D012462:Saline Solution, Hypertonic; D002245:Carbon Dioxide