title: Double adenomas of the pituitary: transcription factors Pit-1, T-pit, and SF-1 identify cytogenesis and differentiation.
abstract: The diagnosis of double adenomas of the pituitary can be very complex and is usually suspected on histological assessment of a specimen and confirmed by immunohistochemical and ultrastructural studies. The most commonly applied technique is currently immunohistochemical staining to localize the six pituitary hormones. Application of this technique may fail to identify double adenomas when hormone immunoreactivity is weak or absent in one or both cell populations. We examined specimens from eight patients diagnosed with double adenomas over a 15-yr period. We tested the ability to detect the difference in the two adenomas in each case using three immunostains for the pituitary transcription factors Pit-1, T-pit, and SF-1. We conclude that immunohistochemical localization of the transcription factors Pit-1, T-pit, and SF-1 accurately detects and classifies the distinct cytodifferentiation of double adenomas of the pituitary.
authors.affiliation: Department of Pathology, University Health Network & Toronto Medical Laboratories, and Department of Laboratory Medicine and Pathobiology, University of Toronto.
authors.forename: R A
authors.initials: RA
authors.lastname: Jastania
authors.forename: K O
authors.initials: KO
authors.lastname: Alsaad
authors.forename: M
authors.initials: M
authors.lastname: Al-Shraim
authors.forename: K
authors.initials: K
authors.lastname: Kovacs
authors.forename: S L
authors.initials: SL
authors.lastname: Asa
mesh_terms: D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic; D005260:Female; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D016609:Neoplasms, Second Primary / Q000175:diagnosis / Q000378:metabolism*; D010911:Pituitary Neoplasms / Q000378:metabolism* / Q000473:pathology; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis*; D012189:Retrospective Studies; D054339:Steroidogenic Factor 1; D020825:T-Box Domain Proteins; D050817:Transcription Factor Pit-1 / Q000096:biosynthesis*; D014157:Transcription Factors / Q000096:biosynthesis*
chemical_list: D018398:Homeodomain Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D020825:T-Box Domain Proteins; C486589:TBX19 protein, human; D050817:Transcription Factor Pit-1; D014157:Transcription Factors