[Cloning and expression of S9-1 gene of rice black-streaked dwarf virus in Escherichia coli].
The cDNA of RBSDV S9 was amplified by RT-PCR using the total RNA of infected maize leaves as the template, and then the PCR product was cloned into pBluescriptII SK. After the DNA sequence was determined, the S9-1 gene was subcloned into E. coli expression vector pET-21d. SDS-PAGE analysis revealed that a 40 kD protein was highly expressed. N-terminal sequence analysis confirmed the 40 kD protein was S9-1 protein. Antiserum was prepared using the purified protein. Western blotting indicated that S9-1 protein could be detected in the infected maize leaves.
Li
Chunbo
C
The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
Zhong
Yongwang
Y
Zhang
Xudong
X
Wei
Chunhong
C
Li
Yi
Y
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008970:Molecular Weight; D010935:Plant Diseases / Q000821:virology; D012087:Reoviridae / Q000235:genetics*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003313:Zea mays / Q000821:virology
D014764:Viral Proteins