A novel crossed microfluidic device for the precise positioning of proteins and vesicles.
Herein we present a novel way to create arrays of different proteins or lipid vesicles using a crossed microfluidic device. The concept relies on the combination of I) a designated two-step surface chemistry, which allows activation for subsequent binding events, and II) crossing microfluidic channels for the local functionalization by separated laminar streams. Besides its simplicity and cost efficiency, this concept has the advantage that it keeps the proteins in a hydrated environment throughout the experiment. We have demonstrated the feasibility of such a device to create a chessboard pattern of different fluorescently labeled lipid vesicles, which offers the possibility to contain biomolecules, drugs or membrane proteins.
Dusseiller
Marc R
MR
Swiss Federal Institute of Technology (ETHZ) Zurich, Department of Materials, Laboratory for Surface Science and Technology, BioInterface Group, CH-8093 Zurich, Switzerland.
Niederberger
Brigitte
B
Städler
Brigitte
B
Falconnet
Didier
D
Textor
Marcus
M
Vörös
Janos
J
D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D008081:Liposomes / Q000737:chemistry*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D010865:Pilot Projects; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*
D008081:Liposomes; D011506:Proteins