[Analyses of Streptomyces coelicolor inner membrane proteome by multidimentional protein identification technology].
Streptomyces coelicolor is the model species among streptomycetes. Until now, proteomic analyses of S. coelicolor have been conducted using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry method, few integral membrane proteins were identified due to the hydrophobic and low-abundance nature of these proteins. In this work, 154 possible inner membrane proteins from S. coelicolor were identified using high pH-proteinase K sample preparation method and multidimensional protein identification technology, among them 44 are integral membrane proteins containing at least one transmembrane domain, most peptides and their corresponding proteins were identified experimentally for the first time.
Shi
Xuan-Ming
XM
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Luo
Yuan-Ming
YM
Zhang
Gui-Feng
GF
Su
Zhi-Guo
ZG
Huang
Yu-Bi
YB
Yang
Ke-Qian
KQ
D001426:Bacterial Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry; D016680:Genome, Bacterial / Q000235:genetics; D013058:Mass Spectrometry / Q000379:methods*; D008565:Membrane Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D048372:Streptomyces coelicolor / Q000737:chemistry* / Q000235:genetics
D001426:Bacterial Proteins; D008565:Membrane Proteins; D020543:Proteome