Metabolite profiling of Chlamydomonas reinhardtii under nutrient deprivation.
A metabolite profiling technique for Chlamydomonas reinhardtii cells for multiparallel analysis of low-molecular weight polar compounds was developed. The experimental protocol was optimized to quickly inactivate enzymatic activity, achieve maximum extraction capacity, and process large sample quantities. As a result of the rapid sampling, extraction, and analysis by gas chromatography coupled to time-of-flight mass spectrometry, more than 800 analytes from a single sample could be measured, of which more than 100 could be identified. Analyte responses could be determined mostly with ses less than 10%. Wild-type cells of C. reinhardtii strain CC-125 subjected to nitrogen-, phosphorus-, sulfur-, or iron-depleted growth conditions develop highly distinctive metabolite profiles. Individual metabolites undergo marked changes in their steady-state levels. Compared to control conditions, sulfur-depleted cells accumulated 4-hydroxyproline more than 50-fold, whereas the amount of 2-ketovaline was reduced to 2% of control levels. The contribution of each compound to the differences observed in the metabolic phenotypes is summarized in a quantitatively rigorous way by principal component analysis, which clearly discriminates the cells from different growth regimes and indicates that phosphorus-depleted conditions induce a deficiency syndrome quite different from the response to nitrogen, sulfur, or iron starvation.
Bölling
Christian
C
Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany. boelling@mpimp-golm.mpg.de
Fiehn
Oliver
O
D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry; D010641:Phenotype
D003470:Culture Media