title: 26-Locus Y-STR typing in a Bhutanese population sample.
abstract: 26 Y chromosome short tandem repeat (STR) loci were amplified in a sample of 856 unrelated males from Bhutan, using two multiplex polymerase chain reaction (PCR) assays. The first multiplex is the Y-STR 20plex described by Butler et al. [J.M. Butler, R. Schoske, P.M. Vallone, M.C. Kline, A.J. Redd, M.F. Hammer, A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers, Forensic Sci. Int. 129 (2002) 10-24], and the second is a novel (but overlapping) 14plex that targets six additional Y-STRs (DYS425, DYS434, DYS435, DYS436, DYS461, DYS462) and also amplifies the amelogenin locus. The 26-loci give a discriminating power of 0.9957, though even at this resolution one haplotype occurs 24 times. We identify novel alleles at five loci and microvariants at a further three, which were characterised by sequencing. Extended (11-locus) haplotypes for these samples have been submitted to the Y-STR Haplotype Reference Database (YHRD).
authors.affiliation: Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.
authors.forename: Emma J
authors.initials: EJ
authors.lastname: Parkin
authors.forename: Thirsa
authors.initials: T
authors.lastname: Kraayenbrink
authors.forename: George L
authors.initials: GL
authors.lastname: van Driem
authors.forename: Karma
authors.initials: K
authors.lastname: Tshering Of Gaselô
authors.forename: Peter
authors.initials: P
authors.lastname: de Knijff
authors.forename: Mark A
authors.initials: MA
authors.lastname: Jobling
mesh_terms: D053523:Amelogenin; D001631:Bhutan; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting; D003746:Dental Enamel Proteins / Q000235:genetics; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
chemical_list: D053523:Amelogenin; D003746:Dental Enamel Proteins