Quantitative and qualitative analysis of flavonoid markers in Frucus aurantii of different geographical origin by capillary electrophoresis with electrochemical detection.
A capillary electrophoresis with electrochemical detection (CE-ED) method was developed for the simultaneous determination of four major flavonoid markers (synephrine, naringin, hesperidin and naringenin) in Frucus aurantii of different geographical origin. Operated in a wall-jet configuration, a 300 microm diameter carbon disc electrode was used as the working electrode, which exhibits a good response at +0.85 V (versus saturated calomel electrode) for the analytes. Under the optimum conditions, the analytes were baseline separated within 20 min in a 80 mmol/L borax buffer (pH 8.45). The intra-day relative standard deviations (R.S.D.) and inter-day R.S.D.s were based on the analysis of the standard solution on the same day and on the following 6 consecutive days. The intra-day R.S.D.s ranged from 0.8% (naringin) to 3.6% (hesperidin). The inter-day R.S.D.s ranged from 1.2% (hesperidin) to 4.6% (naringenin). Calibration curves were linear in ranges between 0.05 and 1000 microg/mL for the markers. Limits of detection ranged from a low of 1 x 10(-8)g/mL (hesperidin) to a high of 5 x 10(-7)g/mL (naringin). The method was successfully used in the analysis of F. aurantii of different geographical origin with relatively simple extraction procedures, and the assay results were satisfactory.
Peng
Youyuan
Y
Department of Chemistry, Quanzhou Normal University, Quanzhou 362000, China. youyuanpeng@hotmail.com
Liu
Fanghua
F
Ye
Jiannong
J
D002021:Buffers; D002681:China; D002957:Citrus / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D006569:Hesperidin / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D013578:Synephrine / Q000032:analysis; D013997:Time Factors
D002021:Buffers; D044950:Flavanones; D005419:Flavonoids; D006569:Hesperidin; C005273:naringenin; C005274:naringin; D013578:Synephrine