Development of efficient expression system for protein display on bacterial magnetic particles.
Bacterial magnetic particles (BMPs) are utilized for various biomedical applications because they are easily manipulated by magnets, and functional proteins are easily displayed on BMPs. To establish highly expressed protein display on BMPs, strong promoters were identified using Magnetospirillum magneticum AMB-1 genome and proteome databases. Initially, several proteins highly expressed in AMB-1 were identified, and the upstream DNA sequences of the open-reading frames were evaluated using a luciferase-reporter gene assay to compare promoter activities. Consequently, luminescence intensity was 400 times higher due to the novel promoter identified in this study than the magA promoter previously used. Subsequently, efficient protein display on BMPs was performed using the newly identified promoter sequences. This developed display system will facilitate the assembly of various functional proteins onto BMPs to create novel magnetic nanoparticles.
Yoshino
Tomoko
T
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.
Matsunaga
Tadashi
T
D001426:Bacterial Proteins / Q000096:biosynthesis*; D027682:Cation Transport Proteins / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D008280:Magnetics; D042201:Magnetospirillum / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics
D001426:Bacterial Proteins; D027682:Cation Transport Proteins; C097085:MagA protein, Magnetospirillum