[RT--PCR analysis of Phanerochaete chrysosporium lip genes in colonized fir wood].
Expression of Phanerochaete chrysosporium lip A2 (GLG3) lip C1 (GLG2) lip C2 (GLGS) lipD2( GLG1), lipE (LP0811) genes were analyzed by RT-PCR method. It was showed that some genes were expressed in special colonized period. Only lip A2 (GLG3) transcription occured in the 2nd week and the 8th week, both lip C1 (GLG2) and lip D2 (GLG1) gene transcription was checked out in the period of 6 weeks. However, no lip genes were expressed in the time of 4 weeks. These results indicated that lip gene expression is relied on the colonized period and transcript patterns are re dramatically different from those in previous studies with defined media.
Jiang
Mingfeng
M
The College of Life Science, Sichuan University, Chengdu 610064, China. jmfmbl@sina.com
Zhang
Yizheng
Y
D028202:Abies / Q000821:virology*; D010544:Peroxidases / Q000235:genetics*; D020075:Phanerochaete / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D014158:Transcription, Genetic; D014934:Wood / Q000821:virology*
D010544:Peroxidases; C042858:lignin peroxidase