title: In vivo regeneration of small-diameter (2 mm) arteries using a polymer scaffold.
abstract: The difficulty of obtaining significant long-term patency and good wall mechanical strength in vivo has been a significant obstacle in achieving small-diameter vascular prostheses. The aim of the present study was to develop a prosthetic graft that could perform as a small-diameter vascular conduit. Tubular structures of hyaluronan (HYAFF-11 tubules, 2 mm diameter, 1 cm length) were grafted in the abdominal aorta of 30 rats as temporary absorbable guides to promote regeneration of vascular structures. Performance was assessed by histology, immunohistochemistry, and ultra-structural analysis. These experiments resulted in three novel findings: 1) complete endothelialization of the tube's luminal surface occurred; 2) sequential regeneration of vascular components led to complete vascular wall regeneration 15 days after surgery; and 3) the biomaterial used created the ideal environment for the delicate regeneration process during the critical initial phases, yet its biodegradability allowed for complete degradation of the construct four months after implantation, at which time, a new artery remained to connect the artery stumps. This study assesses the feasibility to create a completely biodegradable vascular regeneration guide in vivo, able to sequentially orchestrate vascular regeneration events needed for very small artery reconstruction.
authors.affiliation: Clinic of Vascular Surgery, Padova, Italy.
authors.forename: Sandro
authors.initials: S
authors.lastname: Lepidi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Abatangelo
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Vindigni
authors.forename: Giovanni Paolo
authors.initials: GP
authors.lastname: Deriu
authors.forename: Barbara
authors.initials: B
authors.lastname: Zavan
authors.forename: Carolin
authors.initials: C
authors.lastname: Tonello
authors.forename: Roberta
authors.initials: R
authors.lastname: Cortivo
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001012:Aorta, Abdominal / Q000378:metabolism / Q000601:surgery*; D001158:Arteries / Q000254:growth & development* / Q000637:transplantation*; D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*; D014654:Vascular Patency
chemical_list: D001672:Biocompatible Materials; D006820:Hyaluronic Acid