Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat (Triticum dicoccoides).
Three low-molecular-weight glutenin subunit (LMW-GS) genes, designated LMW-Td1, LMW-Td2 and LMW-Td3, were isolated from wild emmer wheat (Triticum dicoccoides), which is the tetraploid progenitor of common wheat (T. aestivum). The complete nucleotide sequence lengths of LMW-Td1, LMW-Td2 and LMW-Td3 are 858, 900 and 1062 bp, respectively. LMW-Td1 and LMW-Td3 can encode proteins with 284 and 352 amino acid residues, respectively, whereas LMW-Td2 is a putative pseudogene due to the presence of 3 inframe stop codons in its C-terminal domain. The deduced protein sequences of the 3 genes share the same typical polypeptide structures with known LMW-GS genes containing 8 cysteines in the mature protein domains. LMW-Td1 was clearly distinguished from all known LMW-GS genes, and considered as a novel LMW-GS gene. Two hydrophobic motifs (i.e. PIIIL and PVIIL) were observed in the repetitive domain of LMW-Td3. Sequence comparison indicates that sequences of the 3 LMW-GS genes from this study are strongly similar to known LMW-GS genes. Our phylogenetic analysis suggests that LMW-Td1 and LMW-Td2 are homologous with genes on chromosome 1A, and LMW-Td3 is closely related to genes on chromosome 1B.
Yue
Yuan-Wen
YW
Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan 611830, China.
Long
Hai
H
Liu
Qian
Q
Wei
Yu-Ming
YM
Yan
Ze-Hong
ZH
Zheng
You-Liang
YL
D000595:Amino Acid Sequence; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers; D005983:Glutens / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers; D021122:Protein Subunits; D005983:Glutens; C037302:glutenin