title: Locked nucleic acid molecular beacons.
abstract: A novel LNA-MB (molecular beacon based on locked nucleic acid bases) has been designed and investigated. It exhibits very high melting temperature and is thermally stable, shows superior single base mismatch discrimination capability, and is stable against digestion by nuclease and has no binding with single-stranded DNA binding proteins. The LNA-MB will be widely useful in a variety of areas, especially for in vivo hybridization studies.
authors.affiliation: Center for Research at Bio/nano Interface, Department of Chemistry, UF Genetics Institute, Gainesville, Florida 32611-7200, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
authors.forename: Colin D
authors.initials: CD
authors.lastname: Medley
authors.forename: Steven A
authors.initials: SA
authors.lastname: Benner
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D005453:Fluorescence; D020869:Gene Expression Profiling; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D009711:Nucleotides; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; C477371:locked nucleic acid