title: Intein-mediated, in vitro and in vivo protein modifications with small molecules.
abstract: We review intein-mediated approaches for the site-specific modifications of proteins and highlight their applications in (1) the site-specific in vitro and in vivo biotinylation of proteins for protein arrays and (2) the site-specific in vivo labeling of proteins in living cells.
authors.affiliation: Department of Biological Sciences, National University of Singapore, 3, Science Drive 3, Singapore 117543, Republic of Singapore.
authors.forename: Lay Pheng
authors.initials: LP
authors.lastname: Tan
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D001710:Biotin / Q000737:chemistry; D019921:Biotinylation / Q000379:methods; D002474:Cell-Free System; D049452:Green Fluorescent Proteins / Q000737:chemistry; D047668:Inteins / Q000502:physiology*; D040081:Protein Array Analysis / Q000379:methods; D015202:Protein Engineering / Q000379:methods; D046188:Protein Modification, Translational / Q000502:physiology*; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods
chemical_list: D011506:Proteins; D049452:Green Fluorescent Proteins; D001710:Biotin