title: The Pseudomonas aeruginosa proteome during anaerobic growth.
abstract: Isotope-coded affinity tag analysis and two-dimensional gel electrophoresis followed by tandem mass spectrometry were used to identify Pseudomonas aeruginosa proteins expressed during anaerobic growth. Out of the 617 proteins identified, 158 were changed in abundance during anaerobic growth compared to during aerobic growth, including proteins whose increased expression was expected based on their role in anaerobic metabolism. These results form the basis for future analyses of alterations in bacterial protein content during growth in various environments, including the cystic fibrosis airway.
authors.affiliation: Department of Medicine, Microbiology, and Genome Sciences, University of Washington, Seattle, 98195, USA.
authors.forename: Manhong
authors.initials: M
authors.lastname: Wu
authors.forename: Tina
authors.initials: T
authors.lastname: Guina
authors.forename: Mitchell
authors.initials: M
authors.lastname: Brittnacher
authors.forename: Hai
authors.initials: H
authors.lastname: Nguyen
authors.forename: Jimmy
authors.initials: J
authors.lastname: Eng
authors.forename: Samuel I
authors.initials: SI
authors.lastname: Miller
mesh_terms: D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins