title: Procollagen alpha 1 type 1: a potential aide in histopathological grading of glioma.
abstract: Collagen type I production has been shown to play a role in malignant transformation. We examined procollagen type I expression in brain tumors and with histopathological grading. Expression levels of procollagen alpha 1 type 1 were determined in 5 glioma cell lines by RT-PCR, Northern, and Western blot analysis. In addition, 41 primary brain tumors and 2 metastatic lung cancers to the brain were examined by PCR. Of the 5 glioma cell line analyzed, 3 (glioma 1, SW-1783 and U-118) expressed procollagen alpha 1 type I and were sensitive to vitamin D3 (VD3). In contrast, 2 of the cell lines (U-373 and T-98G) lacked procollagen alpha 1 type 1 expression. In patients' samples, 14 of 15 anaplastic and low grade gliomas expressed procollagen alpha 1 type I, and 12 of the 14 expressed high levels. In contrast, only 12 of 21 high grade gliomas from patients expressed procollagen alpha 1 type1 and among these, only 4 of the 12 expressed high levels. Thus, there is an inversed correlation between procollagen alpha 1 type 1 expression and histopathological grading (R2=- 0.56, p=0.0005). Our data suggest that procollagen alpha 1 type I expression occurs more commonly in intermediate and low grade gliomas and may assist in histopathological grading.
authors.affiliation: VA Medical Center, Miami, Florida 33125, USA. nsavaraj@med.miami.edu
authors.forename: Niramol
authors.initials: N
authors.lastname: Savaraj
authors.forename: Chunjing
authors.initials: C
authors.lastname: Wu
authors.forename: Howard
authors.initials: H
authors.lastname: Landy
authors.forename: Medhi
authors.initials: M
authors.lastname: Wangpaijit
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Wei
authors.forename: M Tien
authors.initials: MT
authors.lastname: Kuo
authors.forename: Carlos
authors.initials: C
authors.lastname: Robles
authors.forename: A J
authors.initials: AJ
authors.lastname: Furst
authors.forename: Theodore
authors.initials: T
authors.lastname: Lampidis
authors.forename: Lynn
authors.initials: L
authors.lastname: Feun
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000096:biosynthesis*; D001932:Brain Neoplasms / Q000473:pathology*; D024042:Collagen Type I / Q000032:analysis / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D005910:Glioma / Q000473:pathology*; D006801:Humans; D009367:Neoplasm Staging / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D024042:Collagen Type I