title: Catalytic activity of autoantibodies toward myelin basic protein correlates with the scores on the multiple sclerosis expanded disability status scale.
abstract: Autoantibodies toward myelin basic protein (MBP) evidently emerge in sera and cerebrospinal fluid of the patients with multiple sclerosis (MS), as well as in a MS rodent model, i.e., experimental autoimmune encephalomyelitis (EAE). The studies of the last two decades have unveiled somewhat controversial data on the diagnostic applicability of anti-MBP autoantibodies as a disease' marker. Here, we present the results of new functional analysis of the anti-MBP autoantibodies isolated from MS (in patients) and EAE (in mice) sera, based on their proteolytic activity against the targeted autoantigen. The activity was shown to be the intrinsic property of the IgG molecule. No activity was found in the sera-derived antibody fraction of healthy donors and control mice. Sera of 24 patients with clinically proven MS at different stages of the disease, and 20 healthy controls were screened for the anti-MBP antibody-mediated proteolytic activity. The activity correlated with the scores on the MS expanded disability status scale (EDSS) (r(2)=0.85, P<0.001). Thus, the anti-MBP autoantibody-mediated proteolysis may be regarded as an additional marker of the disease progression.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 16/10, Miklukho-Maklaya str., Moscow 117997, Russia.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Ponomarenko
authors.forename: Oxana M
authors.initials: OM
authors.lastname: Durova
authors.forename: Ivan I
authors.initials: II
authors.lastname: Vorobiev
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Belogurov
authors.forename: Georgy B
authors.initials: GB
authors.lastname: Telegin
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Suchkov
authors.forename: Victor K
authors.initials: VK
authors.lastname: Misikov
authors.forename: Herbert C
authors.initials: HC
authors.lastname: Morse
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Gabibov
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D017151:Antibodies, Catalytic; D001323:Autoantibodies / Q000097:blood / Q000378:metabolism*; D002384:Catalysis; D004185:Disability Evaluation; D004681:Encephalomyelitis, Autoimmune, Experimental; D006801:Humans; D051379:Mice; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000175:diagnosis / Q000209:etiology*; D004676:Myelin Basic Protein / Q000097:blood / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000097:blood / Q000378:metabolism; D012720:Severity of Illness Index
chemical_list: D017151:Antibodies, Catalytic; D001323:Autoantibodies; D004676:Myelin Basic Protein; D010447:Peptide Hydrolases