title: Pyruvate improves neutrophilic nitric oxide generation in peritoneal dialysis solutions.
abstract: To investigate the effects of pyruvate (Pyr)-based peritoneal dialysis solutions (P-PDS) on neutrophilic nitric oxide (NO) generation, we incubated human peripheral neutrophils in dL-lactate (Lac, 40 mM)-based PDS and equimolar P-PDS, and Hanks' balanced salt solution at various pH and high glucose (HG) levels, respectively. The production of NO in various test solutions was determined based on the Griess reaction. Acidic pH, high Lac, and HG induced an acute and substantial inhibition of neutrophilic NO, whereas Pyr in PDS significantly improved the NO generation in both acidic pH and physiological pH, and also in HG conditions. The Pyr protection may be associated with the improvement of glucose metabolic pathways in addition to its alkalization.
authors.affiliation: Department of Nephrology, Tong Ji Hospital, Tong Ji University School of Medicine, Shanghai, China.
authors.forename: Yi-Tai
authors.initials: YT
authors.lastname: Wu
authors.forename: Zhao-Long
authors.initials: ZL
authors.lastname: Wu
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Jiang
authors.forename: Su
authors.initials: S
authors.lastname: Li
authors.forename: Fang-Qiang
authors.initials: FQ
authors.lastname: Zhou
mesh_terms: D002470:Cell Survival; D002478:Cells, Cultured; D015314:Dialysis Solutions / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D010530:Peritoneal Dialysis; D019289:Pyruvic Acid / Q000494:pharmacology*; D013053:Spectrophotometry
chemical_list: D015314:Dialysis Solutions; D007773:Lactates; D009569:Nitric Oxide; D019289:Pyruvic Acid; D005947:Glucose