title: Immunogenicity and protective efficacy of recombinant vaccine based on the receptor-binding domain of the Plasmodium vivax Duffy binding protein in Aotus monkeys.
abstract: Invasion of human erythrocytes by Plasmodium vivax requires interaction between Duffy binding protein (PvDBP) and the Duffy blood group antigen. The receptor-binding domain of PvDBP lies in a conserved N-terminal, cysteine-rich region, region II (PvRII). PvRII is a valuable malaria subunit vaccine candidate for asexual blood stages. We have evaluated in Aotus monkeys the immunogenicity and protective efficacy of recombinant PvRII formulated in Freund's and Montanide ISA720 adjuvants. Specific antibody titers were determined by an enzyme-linked immunosorbent assay after each of three doses of 50 microg of protein administered by the subcutaneous route. Immunization with PvRII formulated in Freund's adjuvant yielded higher antibody titers than immunization with the Montanide ISA720 formulation and offered partial protection. Although the Montanide ISA720 formulation was immunogenic, it did not provide any protection. Given the immunogenicity and partial protection observed, further studies are needed to optimize the PvRII vaccine formulation with adjuvants suitable for human use.
authors.affiliation: Institute of Immunology, Universidad del Valle, Cali, Colombia. marevalo@immuno.org
authors.forename: Myriam
authors.initials: M
authors.lastname: Arévalo-Herrera
authors.forename: Angélica
authors.initials: A
authors.lastname: Castellanos
authors.forename: Syed S
authors.initials: SS
authors.lastname: Yazdani
authors.forename: Ahmad Rushdi
authors.initials: AR
authors.lastname: Shakri
authors.forename: Chetan E
authors.initials: CE
authors.lastname: Chitnis
authors.forename: Rosalie
authors.initials: R
authors.lastname: Dominik
authors.forename: Sócrates
authors.initials: S
authors.lastname: Herrera
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D002427:Cebidae; D004195:Disease Models, Animal; D004375:Duffy Blood-Group System / Q000378:metabolism*; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006801:Humans; D007114:Immunization; D017780:Malaria Vaccines / Q000008:administration & dosage / Q000276:immunology*; D016780:Malaria, Vivax / Q000469:parasitology / Q000517:prevention & control*; D008297:Male; D008353:Mannitol / Q000008:administration & dosage / Q000031:analogs & derivatives; D009829:Oleic Acids / Q000008:administration & dosage; D010966:Plasmodium vivax / Q000276:immunology / Q000472:pathogenicity; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D004375:Duffy Blood-Group System; C066001:Duffy antigen binding protein, Plasmodium; D017780:Malaria Vaccines; D009829:Oleic Acids; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D014614:Vaccines, Synthetic; C001681:mannide monooleate; D008353:Mannitol; D005620:Freund's Adjuvant