title: Altered glycosylation of acetylcholinesterase in Creutzfeldt-Jakob disease.
abstract: Changes in the glycosylation pattern of brain proteins have been associated with Creutzfeldt-Jakob disease (CJD). We have investigated the glycosylation status of acetylcholinesterase (AChE) by lectin binding assay. Our data show that in lumbar CSF from definite and probable sporadic CJD cases AChE activity is lower compared with that in age-matched controls. We also show, for the first time, that AChE glycosylation is altered in CJD CSF and brain. Unlike Alzheimer's disease, in which an alteration in both the glycosylation and levels of AChE molecular forms is observed, the abnormal glycosylation of AChE in CJD appears to be unrelated to changes in molecular forms of this enzyme. These findings suggest that altered AChE glycosylation in CJD may be a consequence of the general perturbation of the glycosylation machinery that affects prion protein, as well as other proteins. The diagnostic potential of these changes remains to be explored.
authors.affiliation: Instituto de Neurociencias de Alicante, Universidad Miguel Hernández-CSIC, San Juan de Alicante, Spain.
authors.forename: María-Ximena
authors.initials: MX
authors.lastname: Silveyra
authors.forename: Natividad
authors.initials: N
authors.lastname: Cuadrado-Corrales
authors.forename: Alberto
authors.initials: A
authors.lastname: Marcos
authors.forename: María-Sagrario
authors.initials: MS
authors.lastname: Barquero
authors.forename: Alberto
authors.initials: A
authors.lastname: Rábano
authors.forename: Miguel
authors.initials: M
authors.lastname: Calero
authors.forename: Javier
authors.initials: J
authors.lastname: Sáez-Valero
mesh_terms: D000110:Acetylcholinesterase / Q000134:cerebrospinal fluid / Q000737:chemistry / Q000378:metabolism*; D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism; D001921:Brain / Q000201:enzymology; D001923:Brain Chemistry / Q000502:physiology; D007562:Creutzfeldt-Jakob Syndrome / Q000378:metabolism*; D005260:Female; D006031:Glycosylation; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009778:Occipital Lobe / Q000378:metabolism
chemical_list: D037102:Lectins; D000110:Acetylcholinesterase