title: S100A4 regulates E-cadherin expression in oral squamous cell carcinoma.
abstract: S100A4 has multiple functions in cell cycle progression and cell motility, and has been implicated in cancer invasion. In this study, we examined the expression of S100A4, E-cadherin and its related proteins in oral squamous cell carcinoma (SCC) cell lines with different invasive phenotypes, grade 4C and 4D. Furthermore, grade 4C OSC-19 cells expressing E-cadherin were transfected with S100A4-expression vector and the expression of E-cadherin-related proteins in the stable clone was examined to elucidate the relationship between S100A4 and E-cadherin. Constitutive over-expression of S100A4 in stable transformant of OSC-19 (OSC-19/S100A4) cells led to down-regulation of E-cadherin and beta-catenin. Furthermore, grade 4D invasive cell lines (HOC313 and TSU) expressing S100A4 mRNA did not express E-cadherin, P-cadherin, and beta-catenin, while gamma-catenin protein was only weakly expressed. Thus, the mRNA expression of E-cadherin was reversely correlated with S100A4 expression in oral SCCs. Interestingly, vascular endothelial growth factor-C was up-regulated in OSC-19/S100A4 cells. In summary, S100A4-mediated regulation of E-cadherin expression may play an important mechanism in invasion and metastasis of oral SCC.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kanazawa University Graduate School of Medicine, 13-1 Takaramachi, Kanazawa 920-8640, Japan. yiu54022@nifty.com
authors.forename: Makiko
authors.initials: M
authors.lastname: Moriyama-Kita
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Endo
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yonemura
authors.forename: Claus W
authors.initials: CW
authors.lastname: Heizmann
authors.forename: Hisashi
authors.initials: H
authors.lastname: Miyamori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sato
authors.forename: Etsuhide
authors.initials: E
authors.lastname: Yamamoto
authors.forename: Takuma
authors.initials: T
authors.lastname: Sasaki
mesh_terms: D015820:Cadherins / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000378:metabolism*; D015854:Up-Regulation; D042582:Vascular Endothelial Growth Factor C / Q000096:biosynthesis
chemical_list: D015820:Cadherins; D012333:RNA, Messenger; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; D042582:Vascular Endothelial Growth Factor C; C077591:S100A4 protein, human