title: Structural studies of the core region of Aeromonas salmonicida subsp. salmonicida lipopolysaccharide.
abstract: The core oligosaccharide structure of the in vivo derived rough phenotype of Aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, CE-MS and one- and two-dimensional NMR analyses and established as the following: [carbohydrate: see text] where R=alpha-D-Galp-(1-->4)-beta-D-GalpNAc-(1--> or alpha-D-Galp-(1--> (approx. ratio 4:3). Comparative CE-MS analysis of A. salmonicida subsp. salmonicida core oligosaccharides from strains A449, 80204-1 and an in vivo rough isolate confirmed that the structure of the core oligosaccharide was conserved among different isolates of A. salmonicida.
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Eleonora
authors.initials: E
authors.lastname: Altman
mesh_terms: D048409:Aeromonas salmonicida / Q000737:chemistry*; D002240:Carbohydrate Sequence; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000737:chemistry*; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D008070:Lipopolysaccharides