title: [Selection of A strain producing beta-lactam antibiotics acylases].
abstract: A series of substrates analogues containing the same or similar side chain of its substrate have been synthesized and applied to screen cephalosporin acylase producers from a mass of microorganisms. The deacylation products of these analogues can be detected conveniently and used as screening indicators for the cephalosporin-acylase-producing-microorganism. Six strains possessing deacylation activity have been screened out with these substrate analogs. Among them, strain ZH0650 can simultaneously hydrolyze GL-7ACA, NIPAB and other analogues including AD-NABA. Further investigation on this strain confirmed that it could produce at least three acylases, ADNABA acylase, penicillin G acylase and cephalosporin acylase, which were characterized by bioassay with multiple substrate analogues. This is the first report that three different acylases were produced by one strain.
authors.affiliation: Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institutes for, Biological Sciences, Chinese Academy of Sciences, China.
authors.forename: Songcheng
authors.initials: S
authors.lastname: Zhu
authors.forename: Xi
authors.initials: X
authors.lastname: Huang
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhao
authors.forename: Weihong
authors.initials: W
authors.lastname: Jiang
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D006868:Hydrolysis; D010399:Penicillin Amidase / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D010399:Penicillin Amidase