Detection of Salmonella invA by isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) in Zambia.
The isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) is a new isothermal DNA amplification method composed of exo Bca DNA polymerase, RNaseH and DNA-RNA chimeric primers. We detected invA of Salmonella from chicken carcasses, egg yolk and cattle fecal samples. Fifty-three of 59 isolates were invA-positive in ICAN-chromatostrip detection. The result was consistent with those obtained by standard PCR. Salmonella invA was detected in 12 of 14 carcass rinses by ICAN, while in 7 of 14 rinses by standard PCR. These results indicate that ICAN is an efficient, sensitive and simple system to detect invA of Salmonella species in developing countries such as Zambia.
Isogai
Emiko
E
Department of Preventive Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu 1757, Hokkaido 061-0293, Japan. emiko@hoku-iryo-u.ac.jp
Makungu
Chitwambi
C
Yabe
John
J
Sinkala
Patson
P
Nambota
Andrew
A
Isogai
Hiroshi
H
Fukushi
Hideto
H
Silungwe
Manda
M
Mubita
Charles
C
Syakalima
Michelo
M
Hang'ombe
Bernard Mudenda
BM
Kozaki
Shunji
S
Yasuda
Jun
J
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics*; D002417:Cattle; D002645:Chickens / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010063:Ovum / Q000382:microbiology; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013696:Temperature; D015024:Zambia
D001426:Bacterial Proteins; D017931:DNA Primers; C075518:invA protein, Bacteria