Reduced expression of interleukin-2 decreases the frequency of alopecia areata onset in C3H/HeJ mice.
Alopecia areata (AA) is an autoimmune hair loss disease, that can be transferred between C3H/HeJ mice by skin grafting. We explored whether AA susceptibility is influenced by the availability of interleukin (IL)-2, a cytokine with leukocyte activating and regulatory properties. Mice heterozygous for a targeted deletion of IL-2 from the histocompatible C3.129P2(B6)-Il2(tm1Hor) substrain, that produce reduced levels of IL-2, were examined for AA development after grafting skin from AA-affected C3H/HeJ mice. After grafting, nine of 19 (47%) heterozygous IL-2+/-versus 16 of 18 (88%) IL-2+/+ wild-type littermates developed AA. Although dense follicular leukocyte infiltrates were apparent in AA affected wild-type mice, AA-developing IL-2+/- littermates had a reduced leukocyte infiltration, and AA-resistant IL-2+/- mice had no inflammation. Lymph node cell analysis revealed a reduction in leukocyte activation markers in AA-developing IL-2+/- mice. IL-2+/- mice presented with low level expression of cytokines (IL-4, IL-10, interferon-gamma, transforming growth factor-beta), upregulation of tumor necrosis factor receptors, and increased leukocyte apoptosis susceptibility independent of AA expression. In the skin, CD4+ cells and monocytes were reduced; activation markers were not upregulated and very few CD44v3+ or CD44v10+ leukocytes were recovered. Taken together, our data suggest that AA resistance of IL-2+/- mice is because of the failure of activated leukocyte recruitment, thus pointing toward an involvement of IL-2 in AA pathogenesis.
Freyschmidt-Paul
Pia
P
Department of Dermatology, Philipp University, Marburg, Germany. freyschm@mailer.uni-marburg.de
McElwee
Kevin J
KJ
Hoffmann
Rolf
R
Sundberg
John P
JP
Kissling
Sabine
S
Hummel
Susanne
S
Vitacolonna
Mario
M
Kopp-Schneider
Annette
A
Zöller
Margot
M
D000506:Alopecia Areata / Q000235:genetics / Q000276:immunology*; D000818:Animals; D004198:Disease Susceptibility; D017353:Gene Deletion; D006579:Heterozygote; D007376:Interleukin-2 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation / Q000235:genetics; D051379:Mice; D008809:Mice, Inbred C3H; D008817:Mice, Mutant Strains
D007376:Interleukin-2