Redundancy of DNA helicases in p53-mediated apoptosis.
A subset of DNA helicases, the RecQ family, has been found to be associated with the p53-mediated apoptotic pathway and is involved in maintaining genomic integrity. This family contains the BLM and WRN helicases, in which germline mutations are responsible for Bloom and Werner syndromes, respectively. TFIIH DNA helicases, XPB and XPD, are also components in this apoptotic pathway. We hypothesized that there may be some redundancy between helicases in their ability to complement the attenuated p53-mediated apoptotic levels seen in cells from individuals with diseases associated with these defective helicase genes. The attenuated apoptotic phenotype in Bloom syndrome cells was rescued not only by ectopic expression of BLM, but also by WRN or XPB, both 3' --> 5' helicases, but not expression of the 5' --> 3' helicase XPD. Overexpression of Sgs1, a WRN/BLM yeast homolog, corrected the reduction in BS cells only, which is consistent with Sgs1 being evolutionarily most homologous to BLM. A restoration of apoptotic levels in cells from WS, XPB or XPD patients was attained only by overexpression of the specific helicase. Our data suggest a limited redundancy in the pathways of these RecQ helicases in p53-induced apoptosis.
Spillare
E A
EA
Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA.
Wang
X W
XW
von Kobbe
C
C
Bohr
V A
VA
Hickson
I D
ID
Harris
C C
CC
D017209:Apoptosis / Q000502:physiology*; D001816:Bloom Syndrome / Q000201:enzymology; D004265:DNA Helicases / Q000378:metabolism*; D018095:Germ-Line Mutation; D006801:Humans; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D014898:Werner Syndrome / Q000201:enzymology
D016159:Tumor Suppressor Protein p53; D004265:DNA Helicases