Evaluation of urokinase-type plasminogen activator and its receptor in neointima of polyester vascular grafts.
The aim of the study was to assess the expression of urokinase-type plasminogen activator (uPA) and its receptor (uPAR) in neointima of polyester vascular grafts. Anastomotic areas were examined at 1, 4 and 12 months after prosthesis implantation in dogs, as well as in human vascular grafts. Immunohistochemistry was performed for uPA and uPAR. Graft neointima in dogs was positively stained for uPA with increased intensity at 4 and 12 months, whereas uPAR expression appeared at 4 and its intensity was increased at 12 months. Intensive uPA and positive uPAR labeling was shown in human grafts. The results demonstrated that in the early period of the healing process of polyester vascular grafts only uPA is present in the neointima in the region of the graft to adjacent artery anastomosis, whereas in healed grafts in dogs and humans uPAR is found as well.
Kowalewski
Radoslaw
R
Department of Vascular Surgery and Transplantology, Medical University of Bialystok, Bialystok, Poland. korado@2com.pl
Zimnoch
Lech
L
Wojtukiewicz
Marek Z
MZ
Glowinski
Jerzy
J
Glowinski
Stanislaw
S
D000818:Animals; D001807:Blood Vessel Prosthesis; D004285:Dogs; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D055293:Receptors, Urokinase Plasminogen Activator; D017539:Tunica Intima / Q000166:cytology / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis*
C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator