title: [The expression of recombinant protein of human interleukin-24 and its anti-tumor mechanism].
abstract: The hIL24 cDNA sequence was cloned into prokaryotic high expressive vector pET-21a(+) and recombinant hIL24 was expressed in E. coli with IPTG induction. The purified recombinant hIL24 exhibits following functions in HeLa cell: inhibiting cell growth, inducing apoptosis, inducing PMBC to secrete IL-6, TNF-alpha, IFN-r and inhibiting blood vessel formation. Our preliminary results suggest that the apoptosis induced by rhIL24 is through down-regulating expression of anti-apoptosis factor Bcl-2 and activation of mitochondria apoptosis pathway.
authors.affiliation: Cell and Molecular Biology Institute, College of Medicine, SooChow University, Suzhou 215007, China.
authors.forename: Zhen-Min
authors.initials: ZM
authors.lastname: Ye
authors.forename: Ye-Han
authors.initials: YH
authors.lastname: Zhu
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Sheng
authors.forename: Jing-Cheng
authors.initials: JC
authors.lastname: Miao
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Wei
authors.forename: Kang
authors.initials: K
authors.lastname: Wu
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D008928:Mitochondria / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D000099180:Interleukin-24
chemical_list: D007378:Interleukins; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; D000099180:Interleukin-24