Effects of beta-cyclodextrins on the enzymatical hydrolysis of chiral dichlorprop methyl ester.
The effect of beta-cyclodextrins (beta-CDs) on the enzymatical hydrolysis of chiral dichlorprop methyl ester (DCPPM) was studied. Four kinds of beta-cyclodextrins (beta-cyclodextrin, Partly methylated-CD (PM-beta-CD), hydroxypropyl-cyclodextrin (HP-beta-CD) and carboxymethyl-cyclodextrin (CM-beta-CD)) were used. Compared with 100% DCPPM in the absence of betacyclodextrins, the activity of lipase decreased with the increase of Pbetacyclodextrin and PM-Pbetacyclodextrin. However, CM-beta-clodextrin stimulated the lipase activity. The inhibition effect of Pbeta cyclodextrin and PM-5betacyclodextrin on the hydrolysis of DCPPM is affected by many factors other than degree of the methylation blocking the active site of lipase. UV-Vis and Fourier transform infrared (FTIR) spectroscopy studies of the complexation of aqueous DCPPM with beta-CDs provide fresh insight into the molecular structure of the complex and explain the effects of beta-CDs on enzymatical hydrolysis of chiral DCPPM. Data showed that inclusion complexes had formed by complexation of the CM-beta-CD with DCPPM and the solubility of DCPPM was increased in water, which lead to the increased lipase activity.
Wen
Yue-Zhong
YZ
Institute of Environment Science, Zhejiang University, Hangzhou 310029, China. wenyuezhong@zjuem.zju.edu.cn
Zhou
Shan-shan
SS
Fang
Zhao-hua
ZH
Liu
Wei-ping
WP
D015084:2,4-Dichlorophenoxyacetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental; D002849:Chromatography, Gas; D004785:Environmental Pollutants / Q000378:metabolism*; D006540:Herbicides / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D047392:beta-Cyclodextrins / Q000378:metabolism* / Q000494:pharmacology
D004785:Environmental Pollutants; D006540:Herbicides; D047392:beta-Cyclodextrins; D015084:2,4-Dichlorophenoxyacetic Acid; C101283:dichlorprop methyl ester; D008049:Lipase