title: Electrochemical telomerase assay with ferrocenylnaphthalene diimide as a tetraplex DNA-specific binder.
abstract: Spectroscopic studies revealed that ferrocenylnaphthalene diimide (1) can bind to tetraplex DNA at high potassium ion concentration. The tetraplex DNA was stabilized by the binding of 1, and this effect was larger than that of any other tetraplex stabilizers, which are known as a telomerase inhibitor. Quantitative analysis with circular dichroism and a quartz crystal microbalance strongly suggested a 3:1 binding stoichiometry of 1 to the tetraplex DNA. The telomere sequence could be extended by telomerase with the telomerase substrate primer on the surface of an electrode as proven by an increased current signal of 1 bound to the tetraplex DNA formed on the electrode. This is the first example of electrochemical detection of telomerase activity without relying on PCR.
authors.affiliation: Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Sato
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kondo
authors.forename: Takahiko
authors.initials: T
authors.lastname: Nojima
authors.forename: Shigeori
authors.initials: S
authors.lastname: Takenaka
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry* / Q000378:metabolism*; D054856:G-Quadruplexes; D007094:Imides / Q000737:chemistry* / Q000378:metabolism*; D008024:Ligands; D015394:Molecular Structure; D019098:Telomerase / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D016615:Telomere / Q000737:chemistry; D044366:Transition Temperature
chemical_list: D005296:Ferrous Compounds; D007094:Imides; D008024:Ligands; C406363:ferrocenylnaphthalene diimide; D004247:DNA; D019098:Telomerase