Nanosphere induced gene expression in human dendritic cells.
The molecular mechanisms of nanosphere-induced mucosal immunization are important to decipher, since this can form the basis for novel approaches in, e.g., nasal vaccination. In this study, we have investigated the effect of nanospheres as antigen carriers on immature human dendritic cells. The results clearly indicate that tetanus toxoid immobilized nanospheres have a direct effect on human monocyte derived dendritic cells and induce a specific transcriptional profile involving genes crucial for phagocytosis and a protective immune response.
Matsusaki
Michiya
M
Department of Molecular Chemistry, Graduate School of Engineering, Osaka University and CREST, Osaka, Japan.
Larsson
Kristina
K
Akagi
Takani
T
Lindstedt
Malin
M
Akashi
Mitsuru
M
Borrebaeck
Carl A K
CA
D051940:B7-2 Antigen / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D004337:Drug Carriers; D015870:Gene Expression; D020869:Gene Expression Profiling; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D018928:Immunity, Mucosal; D066298:In Vitro Techniques; D043942:Nanotubes; D010587:Phagocytosis; D013745:Tetanus Toxoid / Q000008:administration & dosage
D051940:B7-2 Antigen; D004337:Drug Carriers; D006684:HLA-DR Antigens; D013745:Tetanus Toxoid