Effects of manganese on extracellular levels of dopamine in rat striatum: an analysis in vivo by brain microdialysis.
The aim of this study is to determine the effects of intrastriatal administration of MnCl2, on the extracellular levels of dopamine (DA) and metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in basal conditions and stimulated by depolarization with KCl and pargyline administration. Also, we studied the effect of MnCl2 on extracellular levels of l-Dopa in the presence of aromatic amino acid decarboxylase (AADC) inhibitor 3-hydroxybencilhydracine-HCl (NSD 1015). This study concluded that MnCl2, reduced the basal and K+-stimulated DA-release in striatum, without notably affecting the DOPAC and HVA levels. Intraperitoneal injection of pargyline increased striatal DA levels, decreasing DOPAC and HVA levels. The infusion of MnCl2 removed the increase in DA levels, without affecting DOPAC and HVA levels. Perfusion of NSD 1015 increased the extracellular levels of L-DOPA in striatum, and MnCl2 increased the effect of NSD1015 on L-Dopa.
Vidal
L
L
Department Functional Biology and Health Sciences, University of Vigo, Campus As Lagoas-Marcosende, 36200, Vigo, Spain. lvidal@uvigo.es
Alfonso
M
M
Campos
F
F
Faro
L R F
LR
Cervantes
R C
RC
Durán
R
R
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D002712:Chlorides / Q000008:administration & dosage / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D045604:Extracellular Fluid / Q000737:chemistry*; D005260:Female; D006719:Homovanillic Acid / Q000378:metabolism; D006834:Hydrazines / Q000378:metabolism; D017895:Manganese Compounds / Q000008:administration & dosage / Q000378:metabolism*; D017551:Microdialysis; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D010293:Pargyline / Q000494:pharmacology; D011189:Potassium Chloride / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014793:Visual Cortex / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*
D065105:Aromatic Amino Acid Decarboxylase Inhibitors; D002712:Chlorides; D006834:Hydrazines; D017895:Manganese Compounds; D008996:Monoamine Oxidase Inhibitors; D015102:3,4-Dihydroxyphenylacetic Acid; D011189:Potassium Chloride; D010293:Pargyline; C004297:3-hydroxybenzylhydrazine; C025340:manganese chloride; D004298:Dopamine; D006719:Homovanillic Acid