Pathogenesis of acute lung injury in rats with severe acute pancreatitis.
BACKGROUND
Acute lung injury (ALI) is the most common and severe complication of severe acute pancreatitis (SAP). The elucidation of the mechanism of ALI contributes to the diagnosis and treatment of the illness. In this study, we studied the pathogenesis of ALI in rats with severe acute pancreatitis.


METHODS
The rats were sacrificed at 1, 3, 5, 6, 9 and 12 hours after the establishment of the model of SAP. Pancreas and lung tissues were obtained for pathological study, and examination of microvascular permeability and myeloperoxidase (MPO) examination. The gene expressions of tumor necrosis factor-alpha (TNF-alpha) and intercellular adhesion molecule-1 (ICAM-1) in the pancreas and lung tissues were detected by reverse transcriptase-polymerase chain reaction (RT-PCR).


RESULTS
After the establishment of the SAP model, the degree of pancreatic and lung injury increased gradually along with the gradual increase of MPO activity and microvascular permeability. Gene expressions of TNF-alpha and ICAM-1 in the pancreas rose at 1 hour and peaked at 7 hours. In contrast, their gene expression in the lungs rose slightly at 1 hour and peaked at 9-12 hours.


CONCLUSIONS
An obvious time window existed between SAP and lung injury, which is beneficial to the early prevention of the development of ALI.
Liu
Xue-Min
XM
Department of Hepatobiliary Surgery, First Hospital, Xi'an Jiaotong University, Xi'an 710061, China. a1090224@pub.xaonline.com
Xu
Jun
J
Wang
Zi-Fa
ZF
D000208:Acute Disease; D000818:Animals; D004195:Disease Models, Animal; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D008168:Lung / Q000473:pathology*; D010179:Pancreas / Q000473:pathology; D010195:Pancreatitis / Q000473:pathology*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1