title: Identification of a new HLA-B allele, HLA-B*4443, in a German family.
abstract: A novel human leukocyte antigen (HLA)-B allele is described. The allele was identified in a German blood donor of Caucasian origin. Because high-resolution HLA-typing using sequence-specific primers gave inconclusive results, sequence-based typing was performed. Nucleotide sequences of exons 2 and 3 most closely match with HLA-B*4417 and HLA-B*440301 (99.5% identity). The predicted protein sequence revealed a single amino acid substitution (D156L) compared with the HLA-B*4417 allele but two substitutions (Y113H, D116S) compared with the HLA-B*440301 allele. Therefore, the novel allele has been officially assigned HLA-B*4443 by the WHO Nomenclature Committee. The HLA-B*4443 allele was found with the A*2301, Cw*0401, B*4443, DRB1*0701, DRB4*0107, and DQB1*0202 haplotype.
authors.affiliation: Institute for Transfusion Medicine, University Hospital of Jena, Germany. hansjoerg.thude@med.uni-jena.de
authors.forename: H
authors.initials: H
authors.lastname: Thude
authors.forename: C
authors.initials: C
authors.lastname: Hardt
authors.forename: U
authors.initials: U
authors.lastname: Schorner
authors.forename: S
authors.initials: S
authors.lastname: Ferencik
authors.forename: C
authors.initials: C
authors.lastname: Helfricht
authors.forename: D
authors.initials: D
authors.lastname: Barz
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D005091:Exons / Q000235:genetics*; D005260:Female; D005858:Germany; D015235:HLA-B Antigens / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D044465:White People
chemical_list: D015235:HLA-B Antigens