title: [Oxidative stress-induced differentiation of L6 myoblasts].
abstract: OBJECTIVE
  To explore the relationship between the differentiation of L6 myoblasts and oxidative stress.
  
  
  METHODS
  MTT assay was used to determine the viability of L6 myoblasts, from which the total RNA was extracted for amplification of the myogenin gene fragment by RT-PCR. H(2)O(2)-induced morphological changes of the cells were observed.
  
  
  RESULTS
  The myoblasts treated with low concentration of reactive oxygen (50 micromol/L H(2)O(2)) for 1 h exhibited accelerated cell growth (P<0.05), and treatment with 50 and 150 micromol/L H(2)O(2) induced the gene expression of myogenin, a molecular marker for differentiation of myoblasts. Morphological study revealed myotube formation and accelerated differentiation of the myoblasts induced by H(2)O(2).
  
  
  CONCLUSIONS
  The reactive oxygen may serve as the intracellular signal molecules to induce the growth and differentiation of the myoblasts.
authors.affiliation: Institute of Clinical Anatomy, Southern Medical University, Guangzhou 510515, China. qqiuxzh@yahoo.com.cn
authors.forename: Xiao-zhong
authors.initials: XZ
authors.lastname: Qiu
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Hua
authors.initials: H
authors.lastname: Liao
authors.forename: Li-sheng
authors.initials: LS
authors.lastname: Zhang
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Qin
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Ouyang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D032446:Myoblasts / Q000166:cytology*; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats
chemical_list: D006861:Hydrogen Peroxide