title: Activity of Serratia plymuthica IC1270 gene chiA promoter region in Escherichia coli mutants deficient in global regulators of transcription.
abstract: To study the regulation of expression of the Serratia plymuthica gene chiA encoding a 58-kDa endochitinase, its 586-bp-long upstream regulatory region was cloned, sequenced and fused to a promoterless lac operon in phage lambdaRS45 to obtain a single-copy transcriptional fusion (P F1chiA )-lac in lysogens of Escherichia coli wild-type strains or their mutants deficient in various global regulators of transcription. The level of P F1chiA -lac expression increased about 20- and 90-fold, respectively, in E. coli K12 Deltahns and double Deltahns stpA mutants deficient in H-NS, and in both H-NS and StpA DNA-binding histone-like proteins, as compared to levels in the wild-type strain. In a Deltalrp mutant deficient in the leucine-responsive transcriptional regulator Lrp, the level of P F1chiA -lac expression increased only up to threefold, whereas even smaller differences relative to the wild-type strain were observed in rpoS and Deltacrp mutants deficient in the sigmaS subunit of RNA polymerase and catabolite-repression protein (CRP), respectively. Deletion of the inverted-repeat sequences and curved DNA regions located in the upstream region of chiA essentially did not influence strain IC1270's chiA promoter activity in E. coli .
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, 123182 Moscow, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Khmel
authors.forename: M I
authors.initials: MI
authors.lastname: Ovadis
authors.forename: A V
authors.initials: AV
authors.lastname: Mayatskaya
authors.forename: A M
authors.initials: AM
authors.lastname: Veselovskii
authors.forename: I A
authors.initials: IA
authors.lastname: Bass
authors.forename: V A
authors.initials: VA
authors.lastname: Lipasova
authors.forename: A
authors.initials: A
authors.lastname: Bolshoy
authors.forename: I
authors.initials: I
authors.lastname: Chet
authors.forename: L S
authors.initials: LS
authors.lastname: Chernin
mesh_terms: D001483:Base Sequence; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012705:Serratia / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D002688:Chitinases; C094590:chitinase ChiA