[Expression of BLM mRNA in six tumor cell strains].
The mutations of BLM gene may result in Bloom's syndrome which includes immunodeficiency, predisposition to malignant tumors and so on, and enhances sister chromati exchange (SCE), DNA replication failure, genome instability, and increases cancer susceptibility. This study was aimed to investigate the variability of mRNA expression level and cDNA structure of BLM gene in tumor cell strains so as to look for a new cancerogenic mechanism and to find a new therapeutic target. The expression level of mRNA and the structure of cDNA of BLM gene in six tumor cell strains and the normal human bone marrow mononuclear cells were detected with RT-PCR and DNA sequencing was performed. The results indicated that these tumors cells expressed BLM mRNA higher than the normal human bone marrow mononuclear cells (P < 0.01), but no cDNA sequence abnormality of BLM gene in these tumors cells was observed. It is concluded that the increase of expressing level of BLM mRNA may play an important role in the development of these tumors.
Yi
Xue
X
Department of Hematology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China.
Zou
Ping
P
Liu
Ling-Bo
LB
Tian
Lei
L
Liu
Fang
F
Feng
Xian-Qi
XQ
Xiao
Juan
J
Zhong
Zhao-Dong
ZD
Xiong
Ping
P
D001483:Base Sequence; D045744:Cell Line, Tumor; D004265:DNA Helicases / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D053484:RecQ Helicases; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D012333:RNA, Messenger; C097151:Bloom syndrome protein; D004265:DNA Helicases; D053484:RecQ Helicases