Induction of detoxication enzymes in mice by naturally occurring allyl nitrile.
Little is known about whether glucosinolate-derived nitriles have the ability to increase phase 2 detoxication enzymes and glutathione (GSH) in vivo. In this study, the ability of allyl nitrile, a hydrolysis product of the glucosinolate sinigrin, to increase tissue levels of the phase 2 detoxication enzymes glutathione S-transferase and quinone reductase and GSH in a variety of mouse tissues was examined. At the lowest dose level (11.8 mg/kg/day), allyl nitrile showed inductive ability in the stomach and lungs. At 23.6 mg/kg/day, the inductive effect was observed in the stomach, rectum, urinary bladder, and lungs, whereas at 47.2 mg/kg/day, it was recorded in the stomach, rectum, urinary bladder, kidneys, and lungs. These results show that allyl nitrile displays its maximum potency in the stomach and lungs, which is of interest in light of epidemiological studies demonstrating an inverse association between crucifer intake and the incidence of stomach and lung cancers.
Tanii
Hideji
H
Department of Hygiene, Kanazawa University School of Medicine, 13-1 Takara-machi, Kanazawa 920-8640, Japan. taniih@med.kanazawa-u.ac.jp
Higashi
Tomomi
T
Nishimura
Fusae
F
Higuchi
Yoshihiro
Y
Saijoh
Kiyofumi
K
D000818:Animals; D019607:Brassicaceae / Q000737:chemistry; D004790:Enzyme Induction / Q000187:drug effects; D005982:Glutathione Transferase / Q000096:biosynthesis*; D008168:Lung / Q000201:enzymology; D008297:Male; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis*; D009570:Nitriles / Q000494:pharmacology*; D013270:Stomach / Q000201:enzymology
D009570:Nitriles; C015660:allyl cyanide; D016660:NAD(P)H Dehydrogenase (Quinone); D005982:Glutathione Transferase