DMP1 depletion decreases bone mineralization in vivo: an FTIR imaging analysis.
The role of DMP1 in mineralization was analyzed by comparing bone mineral and matrix properties in dmp1-null female mice to heterozygous and wildtype controls by FTIR imaging spectroscopy. The observed decreased mineral content in dmp1 null mice indicates a key role for dmp1 in bone mineralization. Indirect effects of DMP1 on other systems also determine the KO phenotype.


BACKGROUND
Dentin matrix protein 1 (DMP1), an acidic phosphorylated extracellular matrix protein, is highly expressed in mineralized tissues. In vitro, DMP1 peptides can promote or inhibit mineralization depending on the extent of phosphorylation, the peptide size, and concentration. To clarify the biological function of DMP1 protein on in vivo mineralization, this study analyzed bone properties of dmp1 knockout (KO) mice compared with heterozygous (HET) and wildtype (WT) controls.


METHODS
Tibias from dmp1 KO and age-, sex-, and background-matched HET and WT mice at 4 and 16 weeks (N(total) = 60) were examined by Fourier transform infrared imaging (FTIRI), histology (n = 6 per genotype and age; N = 36), and geometry by muCT (n = 4 per genotype and age; N = 24). Serum ionic calcium and phosphate concentrations were also determined.


RESULTS
The mineral-to-matrix ratios (spectroscopic parameter of relative mineral content) were significantly lower in dmp1 KO mice tibias compared with WT and HET at 4 and 16 weeks. The mineral crystallinity (crystal size/perfection) was significantly increased in dmp1 KO and HET mice relative to WT. Collagen cross-link ratios (a spectroscopic parameter related to the relative amounts of nonreducible/reducible collagen cross-links) in dmp1 KO were not significantly different from WT and HET. Based on muCT, cortical bone cross-sectional areas at 16 but not 4 weeks were significantly reduced in the KO compared with controls. Maximum, minimum, and polar cross-sectional moments of inertia were significantly lower in dmp1 KO than in HET at 16 weeks but not at 4 weeks. Histological analysis and muCT 3-D images suggested that dmp1 KO mice had osteomalacia. Dmp1 KO mice had significantly lower ionic calcium and phosphate concentrations relative to WT, whereas in the HET, values for phosphate were equivalent, and calcium values were decreased relative to WT values.


CONCLUSIONS
The findings of decreased mineral-to-matrix ratio and increased crystal size in bones of dmp1 KO mice suggest that DMP1 has multiple roles (both direct and indirect) in the regulation of postnatal mineralization. We suggest that direct effects on mineral formation, crystal growth, and indirect effects on regulation of Ca x P concentrations and matrix turnover all contribute to the dominant phenotype in the dmp1 KO mouse.
Ling
Yunfeng
Y
Musculoskeletal Integrity Program, Hospital for Special Surgery, New York, New York, USA.
Rios
Hector F
HF
Myers
Elizabeth R
ER
Lu
Yongbo
Y
Feng
Jian Q
JQ
Boskey
Adele L
AL
D000818:Animals; D015519:Bone Density / Q000235:genetics; D001857:Bone Matrix / Q000737:chemistry; D002113:Calcification, Physiologic / Q000235:genetics / Q000502:physiology*; D002118:Calcium / Q000097:blood; D003094:Collagen / Q000737:chemistry; D003460:Crystallization; D018483:Diaphyses / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000502:physiology*; D005260:Female; D006579:Heterozygote; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010018:Osteomalacia / Q000235:genetics / Q000473:pathology; D010710:Phosphates / Q000097:blood; D010750:Phosphoproteins / Q000235:genetics / Q000502:physiology*; D017550:Spectroscopy, Fourier Transform Infrared; D013977:Tibia / Q000737:chemistry / Q000473:pathology
C513308:Dmp1 protein, mouse; D016326:Extracellular Matrix Proteins; D010710:Phosphates; D010750:Phosphoproteins; D003094:Collagen; D002118:Calcium