Preparative isolation and purification of five compounds from the Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc by high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) was applied to the separation and purification of five compounds from the Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc. The crude extracts from P. cuspidatum Sieb. et Zucc were treated with light petroleum-ethyl acetate-methanol-water (2:5:4:6, v/v). Sample 1 was obtained from the lower phase and sample 2 from the upper phase. The sample 1 was separated with light petroleum-ethyl acetate-water (1:5:5, v/v) and yielded 19.3mg of piceid, 17.6 mg of anthraglycoside B from 200mg of sample 1. The sample 2 was separated with light petroleum-ethyl acetate-methanol-water (3:5:4:6, v/v) and light petroleum-ethyl acetate-methanol-water (3:5:7:3, v/v) in a gradient elution and yielded 18.5mg of resveratrol, 35.3mg of emodin and 8.2mg of physcion from 220 mg of sample 2. The purity of each compound is over 95% as determined by HPLC. The chemical structures of these components were identified by (1)H NMR and (13)C NMR.
Chu
Xin
X
Department of Chemistry, College of Chemistry and Chemical Engineering, Liaocheng University, No. 34 Wenhua Road, Liaocheng, Shandong 252059, China.
Sun
Ailing
A
Liu
Renmin
R
D000873:Anthracenes / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D004642:Emodin / Q000031:analogs & derivatives / Q000032:analysis; D045468:Fallopia japonica / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D015394:Molecular Structure; D028321:Plant Preparations / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis
D000873:Anthracenes; D005960:Glucosides; D006027:Glycosides; D028321:Plant Preparations; D013267:Stilbenes; C008905:physcione; D004642:Emodin; D000077185:Resveratrol; C058229:polydatin; C442642:anthraglycoside B