title: Cellular lipid fatty acid pattern heterogeneity between reference and recent food isolates of Listeria monocytogenes as a response to cold stress.
abstract: Cells of four reference strains (Scott A, LO 28, CNL 895807 and ATCC 19115) and of five recent food isolates (A00M011, A00M018, A00M087, A00M092 and A00M123) of Listeria monocytogenes were grown until late exponential phase in Brain Heart Broth at two different temperatures (37 degrees C and 4 degrees C). Our results show that significant differences exist between the cellular lipid fatty acid profile of reference and recent food isolates. Like the reference strains, and in keeping with previous reports on the cellular lipid fatty acid profile of L. monocytogenes, the recent food isolates were characterised by the presence of ai15:0, i15:0 and ai17:0. In addition, the fatty acid ai13:0 was observed in all of the recent food isolates grown at 4 degrees C, whereas only two reference strains, Scott A and LO 28, showed ai13:0 in their cellular lipid fatty acid profile at 4 degrees C. When grown at 4 degrees C, the recent food isolates showed a mean aiC15/aiC17 ratio of 66, while reference strains were characterised by significantly lower ratios, ranging between 4.3 (ATCC 19115) and 28.9 (Scott A). These results showed that all of the recent food isolates, Scott A and LO28 strains use chain length and anteiso-branching (ai15:0) as their major response to cold temperature adaptation. However, the cold adaptation response of reference strains CNL 895807 and ATCC 19115 appears to be different.
authors.affiliation: UMR-INRA SECALIM 1014, Ecole Nationale Vétérinaire de Nantes, Route de Gachet, BP 40706, 44307 Nantes Cedex 03, France.
authors.forename: Margarida Ribeiro
authors.initials: MR
authors.lastname: Neunlist
authors.forename: Michel
authors.initials: M
authors.lastname: Federighi
authors.forename: Michel
authors.initials: M
authors.lastname: Laroche
authors.forename: Danièle
authors.initials: D
authors.lastname: Sohier
authors.forename: Gilles
authors.initials: G
authors.lastname: Delattre
authors.forename: Christine
authors.initials: C
authors.lastname: Jacquet
authors.forename: Nour-Eddine
authors.initials: NE
authors.lastname: Chihib
mesh_terms: D000222:Adaptation, Physiological; D003080:Cold Temperature; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification
chemical_list: D005227:Fatty Acids