[Cloning and expression of the apx III A gene of Actinobacillus pleuropneumonie].
The apx III A gene of Actinobacillus pleuropneumonie (App) was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apx III A gene in pMD18-T was ligated into pBluescrip II SK(+), the recombinant expression plasmid pET-28b/apx III A was constructed and analysed with R. E., the protein of apx III A gene expressed in E. coli BL21 was detected by Western blotting. Based on expressed apx III A protein as antigen, empty expression vector as control, the ELISA to detect antibody against apx III A was developed and was primarily used to detect serum samples.
Chen
Hanyang
H
Labs of Animal Viuras, Huazhong Agariculture University, Wuhan 430070, China. chenhanyang2008@yahoo.com.cn
Liu
Junfa
J
He
Qigai
Q
Xiao
Shaobo
S
Chen
Huanchun
H
D000188:Actinobacillus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D008970:Molecular Weight
C079721:ApxIII toxin protein, Actinobacillus; D001426:Bacterial Proteins