title: Specific determination of threonine in biological samples by gas chromatography with electron capture detection.
abstract: Threonine was oxidized into acetaldehyde at 0 degrees C for 30 min with periodic acid. The acetaldehyde formed was converted to a hydrazone with 2,4-dinitrophenyhydrazine. The hydrazone was extracted with n-heptane and quantified by gas liquid chromatography with electron capture detection. An internal standard, 2-amino-3-hydroxyhexanoic acid, was used. The calibration curve of threonine was linear up to 200 nmol in 200 microl sample solution and the determination limit of threonine was 1 nmol in 200 microl sample solution. The recoveries were 100.0, 94.0 and 100.0% from homogenates of octopus tentacles and blood plasma and rat livers, respectively. This method was applied to the determination of threonine in tissues of rats given threonine and starved octopuses. This threonine determination method has been used for studies on the metabolism of d-lactate.
authors.affiliation: Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Japan.
authors.forename: Kazue
authors.initials: K
authors.lastname: Oka
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kimura
authors.forename: Masato
authors.initials: M
authors.lastname: Otsuka
authors.forename: Shinji
authors.initials: S
authors.lastname: Ohmori
mesh_terms: D000818:Animals; D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D008099:Liver / Q000737:chemistry; D008297:Male; D049831:Octopodiformes; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013217:Starvation; D013912:Threonine / Q000032:analysis* / Q000097:blood
chemical_list: D013912:Threonine