High performance liquid chromatography-tandem mass spectrometry of phospholipid molecular species in eggs from hens fed diets enriched in seal blubber oil.
The total lipid fraction of eggs from hens fed diets enriched in seal blubber oil (1.25-5.0% SBO) was directly analysed with normal-phase high performance liquid chromatography coupled on-line with electrospray ionization ion-trap tandem mass spectrometry (HPLC-ESI-MS-MS) for the identification of the molecular species of phospholipids (PLs). The species of phosphatidylethanolamine (PE) and phosphatidylinositol (PI) were all detected as the [M-H](-) ions. The phosphatidylcholine (PC), sphingomyelin (Sph) and lysophosphatidylcholine (LPC) classes, were detected as formate adducts [M+HCOO](-). Tandem MS of PE and PI showed the loss of the carboxylate anions, and, for PI, also the loss of water and inositol. Product ion spectrum of PC, LPC and Sph contained only the [M-CH(3)](-) ion fragment. Feeding different levels of SBO for 5 weeks resulted in a significant increase of PE, PC and PI molecular species carrying eicosapentaenoic acid (C(20:5 omega3), EPA), docosapentaenoic acid (C(22:5 omega3), DPA) and docosahexaenoic acid (C(22:6 omega3), DHA), but not Sph nor LPC. The highest increase of the omega3/omega6 ratio occurred for PE and PC. On the contrary, PI was less affected by the increase of SBO in the diet.
Pacetti
Deborah
D
Dipartimento di Scienze degli Alimenti, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
Boselli
Emanuele
E
Hulan
Howard W
HW
Frega
Natale G
NG
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D004531:Eggs / Q000032:analysis*; D005260:Female; D008244:Lysophosphatidylcholines / Q000032:analysis; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010716:Phosphatidylinositols / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D046048:Seals, Earless; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013109:Sphingomyelins / Q000032:analysis
D004042:Dietary Fats, Unsaturated; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010743:Phospholipids; D013109:Sphingomyelins; C483858:phosphatidylethanolamine