Mutual regulation between serine and nitric oxide metabolism in human glioblastoma cells.
D-Serine indirectly caused dose- and time-dependent inhibition of neuronal nitric oxide synthase (nNOS) without affecting endothelial nitric oxide synthase (eNOS) in human glioblastoma cell line U87. Activity of D-amino acid oxidase (DAAO), catalyzing the oxidative deamination of d-amino acid, was enhanced by NO in a dose-dependent manner. Recently, we have reported that serine racemase (SR) is inhibited by NO and activated by D-serine through nitrosylation and denitrosylation, respectively [K. Shoji, S. Mariotto, A.R. Ciampa, H. Suzuki, Regulation of serine racemase activity by D-serine and nitric oxide in human glioblastoma cells, Neurosci. Lett., in press]. Thus, the metabolism of both d-serine and NO in U87 cells is functionally correlated in a complex manner. Suppression of NO production by d-serine in U87 cells contrasts its known action in enhancing nNOS in neurons.
Shoji
Kazuo
K
Biochemistry Section, Department of Neuroscience and Vision, University of Verona, Strada Le Grazie, 8, I-37134 Verona, Italy.
Mariotto
Sofia
S
Ciampa
Anna Rosa
AR
Suzuki
Hisanori
H
D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D020239:Receptor Cross-Talk / Q000187:drug effects; D012694:Serine / Q000378:metabolism*
D005456:Fluorescent Dyes; D009569:Nitric Oxide; D012694:Serine; D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III