title: Cross-sectional analysis of the polysaccharide composition in cellulosic fiber materials by enzymatic peeling/high-performance capillary zone electrophoresis.
abstract: A combined enzymatic, chemical, and analytical approach was used to determine the cross-sectional carbohydrate composition in cellulosic fibers. The outer surface of cellulosic fibers was enzymatically removed layer-by-layer with precise quantitative control, and the monosaccharides in the peelings were subsequently analyzed by high-performance capillary electrophoresis (HPCE) after precolumn derivatization with a UV label. This method was applied to dissolving pulps and regenerated cellulose fibers, with special emphasis on the cross-sectional distribution of hemicelluloses. Commercially available enzyme solutions were used, resulting in a reproducible peeling. Significant differences were found in the hemicellulose distribution across the fiber of different dissolving pulps, dependent on both natural source (beech or spruce) and preparation process (acidic sulfite cook or prehydrolysis kraft cook). Among the dissolving pulps, beech prehydrolysis kraft pulp showed the highest enrichment of surface xylan. Similar, albeit smaller, differences were noticed between various regenerated fibers (viscose, viscose Modal, and Lyocell): a thin hemicellulose-rich outermost layer was found in all the regenerated fibers studied.
authors.affiliation: Department of Chemistry & Christian-Doppler-Laboratory, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.
authors.forename: John
authors.initials: J
authors.lastname: Sjöberg
authors.forename: Antje
authors.initials: A
authors.lastname: Potthast
authors.forename: Thomas
authors.initials: T
authors.lastname: Rosenau
authors.forename: Paul
authors.initials: P
authors.lastname: Kosma
authors.forename: Herbert
authors.initials: H
authors.lastname: Sixta
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D006868:Hydrolysis; D046911:Macromolecular Substances / Q000737:chemistry*; D018514:Plant Structures / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D013447:Sulfites / Q000737:chemistry; D013997:Time Factors; D014466:Ultraviolet Rays; D014990:Xylans / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002241:Carbohydrates; D046911:Macromolecular Substances; D011134:Polysaccharides; D013447:Sulfites; D014990:Xylans; C007916:hemicellulose; D002482:Cellulose