Cloning of cDNA encoding canine endothelin receptors and their expressions in normal tissues.
The receptors for endothelin (ET) family, ETA and ETB, were molecularly cloned and the expression of ETA and ETB as well as preproendothelin-1 (PPET-1, precursor of ET-1) was examined in normal canine tissues by RT-PCR. The entire open reading frames of the canine ETA and ETB were shown to encode 427 and 442 amino acid residues, respectively, showing from 87.4 to 97.3% sequence similarity to human, mouse, and rat counterparts. ETA and ETB mRNAs were ubiquitously expressed in a variety of canine tissues in this study and PPET-1 mRNA was detected in the tissues except for heart and liver. It was speculated that ET could play an important role in physiological events in most of the organs.
Yasuda
Nobutaka
N
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Kanagawa, Japan.
Tsukui
Toshihiro
T
Masuda
Kenichi
K
Kawarai
Shinpei
S
Ohmori
Keitaro
K
Maeda
Sadatoshi
S
Tsujimoto
Hajime
H
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017931:DNA Primers; D004285:Dogs / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D017466:Receptors, Endothelin / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D017931:DNA Primers; D017466:Receptors, Endothelin