title: Predisposing HLA-DQ2 and HLA-DQ8 haplotypes of coeliac disease and associated enteropathy in microscopic colitis.
abstract: OBJECTIVE
  To assess the presence of both genetic and serological markers of coeliac disease in patients with microscopic colitis, and whether there was associated enteropathy.
  
  
  METHODS
  HLA-DQ2, HLA-DQ8, serum immunoglobulin A-antiendomysial and immunoglobulin A-anti-tissue transglutaminase antibodies were investigated in 59 patients with microscopic colitis. Seventy healthy subjects acted as the control group. Endoscopic biopsies from the distal duodenum were obtained in DQ2-positive or DQ8-positive patients. Patients with histological changes compatible with gluten-sensitive enteropathy were started on a gluten-free diet.
  
  
  RESULTS
  Seventeen of 70 (24.3%) healthy controls were DQ2-positive. Twelve of 25 (48%) patients with lymphocytic colitis (P = 0.027 versus controls), and 11 of 34 (32.3%) with collagenous colitis (P = 0.38 versus controls) were DQ2-positive. There were no differences in the frequency of DQ8-positivity. The coeliac serology was positive in one patient. Duodenal biopsies were performed in 23 DQ2-positive and/or DQ8-positive patients. None had villous atrophy (Marsh III lesion) (0%; 95% confidence interval, 0-6.1). A Marsh type I lesion was found in four patients. Three of these patients were put on a gluten-free diet with disappearance of diarrhoea.
  
  
  CONCLUSIONS
  The results suggest that there is an association of lymphocytic colitis with HLA-DQ2 genes, which might be relevant in the pathogenesis of this disease. The association of microscopic colitis with Marsh type III coeliac disease seems to be rare, making it unnecessary to routinely screen for coeliac disease in microscopic colitis patients.
authors.affiliation: Department of Gastroenterology, Hospital Universitari Mutua Terrassa, Barcelona, Spain. digestiu@mutuaterrassa.es
authors.forename: Fernando
authors.initials: F
authors.lastname: Fernández-Bañares
authors.forename: Maria
authors.initials: M
authors.lastname: Esteve
authors.forename: Carme
authors.initials: C
authors.lastname: Farré
authors.forename: Antonio
authors.initials: A
authors.lastname: Salas
authors.forename: Montserrat
authors.initials: M
authors.lastname: Alsina
authors.forename: Jaume
authors.initials: J
authors.lastname: Casalots
authors.forename: Jorge
authors.initials: J
authors.lastname: Espinós
authors.forename: Montserrat
authors.initials: M
authors.lastname: Forné
authors.forename: Josep Maria
authors.initials: JM
authors.lastname: Viver
mesh_terms: D000368:Aged; D001323:Autoantibodies / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001706:Biopsy; D002446:Celiac Disease / Q000150:complications / Q000235:genetics*; D046728:Colitis, Microscopic / Q000178:diet therapy / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D004386:Duodenum / Q000473:pathology; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005983:Glutens / Q000008:administration & dosage; D006683:HLA-DQ Antigens / Q000235:genetics*; D006239:Haplotypes; D006650:Histocompatibility Testing; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011446:Prospective Studies
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D005819:Genetic Markers; D006683:HLA-DQ Antigens; C053186:HLA-DQ2 antigen; C109756:HLA-DQ8 antigen; D007070:Immunoglobulin A; D005983:Glutens