title: Selective foam separation of binary protein solution by SDS complexation method.
abstract: A fundamental study about the selective foam separation of protein mixture was carried out. A solution containing two proteins, ovalbumin (OA) and lysozyme (LZ), and an anionic surfactant, sodium dodecyl sulfate (SDS), was adjusted to pH 6.0, which referred to an intermediate state between the isoelectric points of the proteins. The solution was processed by continuous foam separation. The results showed that a proper addition of SDS greatly improved the selective recovery of LZ to OA. The experimental data were well explained by a simple model that most of cationic protein molecules (LZ) are associated with SDS and the adsorption of all the species including LZ-SDS complexes are subjected to Langmuir adsorption isotherm. The results also showed that one of the Langmuir parameters, which means a kind of lyophillic property of adsorbed material, of LZ-SDS complexes was extremely large as compared with that of primary protein.
authors.affiliation: Division of Marine Biosciences, Graduate School of Fisheries Sciences, Hokkaido University, Minato-cho 3-1-1, Hakodate 041-8611, Japan. suzuki@elsie.fish
authors.forename: Akira
authors.initials: A
authors.lastname: Suzuki
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yasuhara
authors.forename: Hideshi
authors.initials: H
authors.lastname: Seki
authors.forename: Hideo
authors.initials: H
authors.lastname: Maruyama
mesh_terms: D000042:Absorption; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000737:chemistry*; D010047:Ovalbumin / Q000737:chemistry*; D044367:Phase Transition; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D010047:Ovalbumin; D009113:Muramidase