Four pregnancies in nonmosaic Klinefelter's syndrome using cryopreserved-thawed testicular spermatozoa.
OBJECTIVE
To investigate feasibility of using cryopreserved-thawed testicular spermatozoa from patients with nonmosaic Klinefelter's syndrome for intracytoplasmic sperm injection (ICSI).


METHODS
Case report.


METHODS
University-based hospital and IVF clinic.


METHODS
Six patients with nonmosaic Klinefelter's syndrome who underwent testicular sperm extraction for ICSI.


METHODS
Microdissection testicular sperm extraction (TESE) and ICSI.


METHODS
We compared results of ICSI using cryopreserved testicular spermatozoa with those previously reported in Klinefelter's syndrome and those in nonobstructive azoospermia patients using cryopreserved testicular spermatozoa at our institution with respect to embryo cleavage rate, implantation rate, and pregnancy outcome.


RESULTS
Four of six patient couples with successful microdissection TESE achieved pregnancy using cryopreserved-thawed testicular spermatozoa. One pregnancy resulted in early-pregnancy abortion, two in delivery of healthy singleton girls, and one delivery of a healthy singleton boy.


CONCLUSIONS
Cryopreserved-thawed testicular spermatozoa can be used successfully for ICSI in patients with nonmosaic Klinefelter's syndrome.
Okada
Hiroshi
H
Department of Urology, Teikyo University School of Medicine, Tokyo, Japan. okada@med.teikyo-u.ac.jp
Goda
Kazumasa
K
Muto
Satoru
S
Maruyama
Osamu
O
Koshida
Mitsunobu
M
Horie
Shigeo
S
D000328:Adult; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007713:Klinefelter Syndrome / Q000235:genetics* / Q000473:pathology*; D008297:Male; D011247:Pregnancy; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology; D013737:Testis / Q000166:cytology* / Q000502:physiology