[Study on submerged culture conditions of lipase inhibitor producing strain].
The optimum medium and fermentations conditions of the lipase inhibitor producing strain Bacillus sp. LF were studied. The optimal medium was selected after single factor test and orthogonal test as follows: lactose 0.8%, peptone 1%, (NH4)2SO4 0.8%, NaCl 0.5%, original pH 7.2. With 4% (V/V) inoculum in 40 mL medium in 250 mL flask, Bacillus sp. LF could reach high yield of lipase inhibitor after 24 h at 30 degrees C. The highest lipase inhibitory activity in broth could reach 52.3%.
Zhong
Weihong
W
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China. wh-zhong@sina.com
Miao
Jianju
J
D001407:Bacillus / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods*; D004791:Enzyme Inhibitors / Q000378:metabolism*; D004795:Enzyme Stability; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D013696:Temperature
D001426:Bacterial Proteins; D003470:Culture Media; D004791:Enzyme Inhibitors; D008049:Lipase