Oligosaccharides produced by submerged cultures of Claviceps africana and Claviceps sorghi.
Oligosaccharides produced by submerged cultures of C. africana and C. sorghi were isolated by semipreparative HPLC. Structure of 6-O-beta-D-fructofuranosyl-D-glucopyranose (blastose), 1,6-bis-O-(beta-D-fructofuranosyl)-alpha-D-glucopyranoside (neokestose) and two sugar alcohols, 1-O-beta-D-fructofuranosyl-D-mannitol (fructosylmannitol) and 1,6-bis-O-(beta-D-fructofuranosyl)-D-mannitol (bisfructosylmannitol) was determined by NMR spectrometry. MALDI TOF MS analysis revealed molecular ions [M+Na]+ that indicate the presence of other tetra- and pentasaccharides (m/z = 689.4 and 851.5, respectively) and corresponding sugar alcohol (m/z = 691.4). Rapid conversion of sucrose into series of oligosaccharides and corresponding sugar alcohols was observed in all tested strains.
Flieger
M
M
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czechia. flieger@biomed.cas.cz
Kantorová
M
M
Halada
P
P
Kuzma
M
M
Pazoutová
S
S
Stodůlková
E
E
Kolínská
R
R
D002851:Chromatography, High Pressure Liquid; D002967:Claviceps / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity; D005285:Fermentation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology; D045868:Sorghum / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013402:Sugar Alcohols / Q000737:chemistry / Q000378:metabolism
D009844:Oligosaccharides; D013402:Sugar Alcohols