[Cloning and expression of Yersinia pestis V antigen in Escherichia coli BL21 (DE3)].
The structure gene of V antigen was amplified from the wild strain of Yersinia pestis by means of PCR, and was cloned into vector pET-42b (+). Then, V antigen was successfully expressed in E. coli BL21(DE3) with the induction of IPTG. The expressing amount of the aim protein is as high as 32.8 percent of the total bacterial protein.
Fu
Linfeng
L
Lanzhou Institute of Biological Products, Lanzhou 730046, China. linfengfu@yahoo.com.cn
Wang
Bingxiang
B
Li
Qiming
Q
Lei
Qing
Q
Jiang
Lin
L
Shen
Xinliang
X
D000942:Antigens, Bacterial / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D052899:Pore Forming Cytotoxic Proteins / Q000235:genetics* / Q000378:metabolism; D015010:Yersinia pestis / Q000235:genetics*
D000942:Antigens, Bacterial; C043908:LcrV protein, Yersinia; D052899:Pore Forming Cytotoxic Proteins