Sensitive determination of phenolic compounds using high-performance liquid chromatography with cerium(IV)-rhodamine 6G-phenolic compound chemiluminescence detection.
A simple, selective and sensitive determination method of 20 phenolic compounds has been developed using high-performance liquid chromatography (HPLC) with chemiluminescence detection. The method is based on the chemiluminescent enhancement by phenolic compound of the cerium(IV)-rhodamine 6G system in sulfuric acid medium. Twenty phenolic compounds were separated on a XDB-C(8) column with a gradient elution using a mixture of methanol and 1.0% acetic acid as a mobile phase. Under the optimized conditions, a linear working range extends 2 orders of magnitude with the relative standard deviations of intra- and inter-day precision below 4.0%, and the detection limits (S/N = 3) were in the range of 1.5-82.1 ng/ml. The chemiluminescence reaction was compatible with the mobile phase of high-performance liquid chromatography. The proposed method has been successfully applied to the assay of phenolic compounds in red wine without any pretreatment.
Zhang
Qunlin
Q
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui.
Cui
Hua
H
Myint
Aung
A
Lian
Mei
M
Liu
Lijuan
L
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods; D009942:Organometallic Compounds / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D012235:Rhodamines / Q000737:chemistry*; D014920:Wine / Q000032:analysis
D009942:Organometallic Compounds; D010636:Phenols; D012235:Rhodamines; C506935:cerium(IV)-rhodamine 6G