title: Membrane currents elicited by angiotensin II in astrocytes from the rat corpus callosum.
abstract: The corpus callosum (CC) is the main white matter tract in the brain. It consists primarily of axons and glial cells. In the present work, membrane currents generated by angiotensin II (Ang II) in cultured astrocytes from the CC of newborn and 3-week-old rats were studied using the whole-cell voltage-clamp technique. After 4 days of culture, approximately 90% of cells were positive to glial fibrillary acidic protein (GFAP), indicating their astrocyte lineage. Ang II elicited inward currents in approximately 20% of cells and outward currents in approximately 4% of cells from the CC for newborn or 3-week-old rats. The main effect of Ang II on astrocytes from the newborn rat CC was a reduction of membrane conductance, by blocking of delayed rectifier K(+) currents in 96% of cells. However, no common action of Ang II was observed in cells from 3-week-old rat CC because the responses were quite variable, suggesting the participation of other ion currents. The partial agonist of AT(2) receptors, CGP-42112A, exerted effects on Ang II responses, whereas the AT(1) antagonist ZD7155 did not, suggesting that Ang II responses in CC astrocytes are predominantly mediated by activation of AT(2) receptors. This study is the first to show electrical responses generated by AT(2) receptors in glial cells from the rat central nervous system, and may help gain a better understanding of the functions of Ang II receptors in astrocytes from the rat CC in particular and of glial cells in general. (c) 2005 Wiley-Liss, Inc.
authors.affiliation: Instituto de Neurobiología. Universidad Nacional Autónoma de México, Campus Juriquilla, Querétaro, México.
authors.forename: Marcelino
authors.initials: M
authors.lastname: Montiel-Herrera
authors.forename: Ricardo
authors.initials: R
authors.lastname: Miledi
authors.forename: Jesús
authors.initials: J
authors.lastname: García-Colunga
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000187:drug effects*; D003337:Corpus Callosum / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D004594:Electrophysiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D008564:Membrane Potentials / Q000187:drug effects; D009287:Naphthyridines / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005904:Glial Fibrillary Acidic Protein; D009287:Naphthyridines; D009842:Oligopeptides; C095905:ZD 7155; D000804:Angiotensin II; C060894:CGP 42112A