Tetrahydrobiopterin as combined electron/proton donor in nitric oxide biosynthesis: cryogenic UV-Vis and EPR detection of reaction intermediates.
The role of tetrahydrobiopterin (BH4) as a cofactor in nitric oxide synthase (NOS) has been the object of intense research in the last few years. It was found that in addition to its established effects on the NOS heme spin state, substrate affinity, and enzyme dimerization, BH4 is required as a one-electron donor to oxyferrous [Fe(II).O2] heme that is formed as an intermediate in the catalytic cycle. Cryogenic spectroscopic techniques proved particularly useful in the identification of this role of BH4 in NO synthesis. With these methods, the mechanism of fast reactions, such as the reaction of ferrous NOS with O2, can be unraveled by lowering the reaction temperature to subzero values. This may not only reduce the rate to such an extent that the reaction can be followed on a time scale from seconds to minutes, but intermediates may be observed that do not accumulate at higher temperatures. Cryogenic ultraviolet-visible (UV-vis) and electron paramagnetic resonance spectroscopy have been applied to clarify why the BH4 analogue 4-amino-tetrahydrobiopterin (4-amino-BH4) is unable to support NO synthesis. In the course of these studies, evidence was gathered supporting a role for BH4 as an obligate proton and electron donor. It is believed that the inhibitory action of 4-amino-BH4 derives from an inability to serve as a proton donor, even though it is perfectly able to serve as an electron donor. In this chapter, the suitability, drawbacks, and advantages of cryogenic methods are discussed.
Gorren
Antonius C F
AC
Department of Pharmacology and Toxicology, Karl-Franzens-University Graz, Austria.
Sørlie
Morten
M
Andersson
K Kristoffer
KK
Marchal
Stéphane
S
Lange
Reinhard
R
Mayer
Bernd
B
D001708:Biopterins / Q000031:analogs & derivatives* / Q000502:physiology; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D004583:Electrons; D007700:Kinetics; D009569:Nitric Oxide / Q000502:physiology*; D011522:Protons; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D011522:Protons; D001708:Biopterins; D009569:Nitric Oxide; C003402:sapropterin