Generic model control of the specific growth rate in recombinant Escherichia coli cultivations.
Generic model control is shown to be a powerful tool for keeping a microbial cultivation process close to its predetermined (optimized) control profile. This is demonstrated at the example of the green fluorescent protein expressed in genetically modified Escherichia coli host cells. It is shown that the process can be run very closely to a predefined complex profile of the specific cell growth rate mu(t). Controlling the experiments at many different growth conditions is a straightforward way of effectively collecting the data necessary for optimization of recombinant protein production systems. Although the process dynamics is rather complex, the model for the controller can be kept quite simple. The control technique, used here for specific growth rate control, is quite universal and can be applied for different biotechnological processes as well.
Jenzsch
Marco
M
Institute of Bioengineering, Martin-Luther University, Halle-Wittenberg, Weinbergweg 22, 06120 Halle (Saale), Germany.
Simutis
Rimvydas
R
Luebbert
Andreas
A
D000465:Algorithms; D018533:Biomass; D003198:Computer Simulation; D003470:Culture Media; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D007700:Kinetics; D008954:Models, Biological; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D015203:Reproducibility of Results
D003470:Culture Media; D011994:Recombinant Proteins