Attenuation of Salmonella enterica Serovar Typhimurium by altering biological functions of murein lipoprotein and lipopolysaccharide.
We constructed Salmonella enterica serovar Typhimurium double-knockout mutants in which either the lipoprotein A (lppA) or the lipoprotein B (lppB) gene was deleted from an msbB-negative background strain by marker exchange mutagenesis. These mutants were highly attenuated when tested with in vitro and in vivo models of Salmonella pathogenesis.
Fadl
A A
AA
Department of Microbiology and Immunology, 301 University Blvd., University of Texas Medical Branch, Galveston, TX 77555-1070, USA.
Sha
J
J
Klimpel
G R
GR
Olano
J P
JP
Galindo
C L
CL
Chopra
A K
AK
D000217:Acyltransferases / Q000235:genetics; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D017353:Gene Deletion; D007106:Immune Sera / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D017270:Lipoprotein(a) / Q000235:genetics / Q000276:immunology; D008074:Lipoproteins / Q000235:genetics* / Q000276:immunology; D051379:Mice; D009154:Mutation; D012480:Salmonella Infections / Q000276:immunology / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*
D001426:Bacterial Proteins; D007106:Immune Sera; D008070:Lipopolysaccharides; D017270:Lipoprotein(a); D008074:Lipoproteins; D000217:Acyltransferases