Expression and hormonal regulation of calcyclin-binding protein (CacyBP) in the mouse uterus during early pregnancy.
Calcyclin-binding protein (Siah-1-Interacting Protein, CacyBP/SIP), is a calcium signaling protein involved in the degradation of beta-catenin, however, little is known about its role in reproductive biology. The present study was to character its temporospatial expression pattern and regulation in mouse uterus and to investigate whether it plays a role in the regulation of normal endometrial events. While prominently expressed in both luminal and glandular epithelia, CacyBP underwent dynamic changes during early pregnancy. CacyBP expression was observed weakly from days 1-4. An intense accumulation in luminal and glandular epithelia as well as decidua surrounding the embryo at later stages (days 5-7) was observed. Most notably, CacyBP accumulation in trophoblast was pronounced at day 7. Using ovariectomized and pseudopregnant mice, we found that progesterone (P(4)) and 17beta-estradiol (E(2)) led to increased expression of CacyBP gene and this could be abolished by Ru486 and tamoxifen, respectively. Antisense oligonucleotides (ODNs) against CacyBP significantly inhibited cultured endometrial stromal cells' (ESCs) apoptosis induced by UV irradiation. Injection of antisense ODNs into mouse uterine horn severely impaired the number of implanted blastocysts. Taken together, our results suggested that CacyBP expression was positively regulated by P(4) and E(2). CacyBP may be involved in the regulation of endometrial cell apoptosis during early pregnancy and play an important role in mouse endometrial events such as pregrancy establishment.
Yang
Yong-Jun
YJ
State Key Laboratory Reproduction Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
Liu
Wei-Min
WM
Zhou
Jia-Xi
JX
Cao
Yu-Jing
YJ
Li
Jing
J
Peng
Sha
S
Wang
Li
L
Yuan
Jiang-Gang
JG
Duan
En-Kui
EK
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D003656:Decidua / Q000187:drug effects / Q000378:metabolism; D004336:Drug Antagonism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D015735:Mifepristone / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010052:Ovariectomy; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D011555:Pseudopregnancy; D012333:RNA, Messenger / Q000378:metabolism; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013629:Tamoxifen / Q000494:pharmacology; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology
C111922:Cacybp protein, mouse; D002135:Calcium-Binding Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D013629:Tamoxifen; D015735:Mifepristone; D011374:Progesterone; D004958:Estradiol