The chemosensitivity to therapy of childhood early B acute lymphoblastic leukemia could be determined by the combined expression of CD34, SPI-B and BCR genes.
We have identified genes differentially expressed in childhood early B acute lymphoblastic leukemia at diagnosis, according to chemosensitivity. Chemosensitive (M1) and chemoresistant (M3) patients present <5% and >25% of residual leukemic blasts at 21 days of treatment, respectively. The expression profiles of 4205 genes for 32 patients included in the FRALLE93 protocol have been determined using microarray. From differential analysis, CD34, SPI-B and BCR distinguished M1 from M3 patients using microarray and RT-PCR data. Linear discriminant analysis (LDA) and cross-validation show that the combined expression of these three genes classify and predict correctly around 90% and 80% of patients, respectively.
Talby
Leila
L
Centre d'Immunologie de Marseille-Luminy (CIML), CNRS-INSERM-Univ. Méditerranée, Campus de Luminy, Case 906, 13288 Marseille Cedex 09, France.
Chambost
Hervé
H
Roubaud
Marie-Christine
MC
N'Guyen
Catherine
C
Milili
Michèle
M
Loriod
Béatrice
B
Fossat
Chantal
C
Picard
Christophe
C
Gabert
Jean
J
Chiappetta
Pierre
P
Michel
Gérard
G
Schiff
Claudine
C
D018943:Anthracyclines / Q000008:administration & dosage; D018952:Antigens, CD34 / Q000096:biosynthesis* / Q000235:genetics; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage; D001215:Asparaginase / Q000008:administration & dosage; D001752:Blast Crisis / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002051:Burkitt Lymphoma / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D003348:Cortisone / Q000008:administration & dosage; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015973:Gene Expression Regulation, Leukemic / Q000235:genetics; D006801:Humans; D007223:Infant; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011237:Predictive Value of Tests; D051562:Proto-Oncogene Proteins c-bcr / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014750:Vincristine / Q000008:administration & dosage
D018943:Anthracyclines; D018952:Antigens, CD34; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C076877:SPIB protein, human; D014750:Vincristine; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr; D001215:Asparaginase; D003348:Cortisone