Comparison of transforming growth factor-beta/Smad signaling between normal dermal fibroblasts and fibroblasts derived from central and peripheral areas of keloid lesions.
BACKGROUND
Keloids are benign proliferations of fibroblasts, but their exact etiology and molecular pathogenesis are unknown.


METHODS
Fibroblasts were isolated from the central and peripheral regions of keloids, and the growth behavior and molecular characteristics of the keloid fibroblasts were compared with those of age-adjusted normal dermal fibroblasts.


RESULTS
Central (but not peripheral) keloid fibroblasts exhibited significantly increased growth and high levels of expression of transforming growth factor-beta (TGF-beta) receptor 1 and Smad 2/3.


CONCLUSIONS
Proliferation of central keloid fibroblasts, which results in keloid formation, appears to mainly involve the TGF-beta/Smad pathway.
Tsujita-Kyutoku
Miki
M
Department of Pathology II, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan.
Uehara
Norihisa
N
Matsuoka
Yoichiro
Y
Kyutoku
Shigeo
S
Ogawa
Yutaka
Y
Tsubura
Airo
A
D000199:Actins / Q000378:metabolism; D030201:Activin Receptors, Type I / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007627:Keloid / Q000378:metabolism / Q000503:physiopathology*; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D015398:Signal Transduction; D012867:Skin / Q000166:cytology; D051785:Smad Proteins / Q000378:metabolism*; D051899:Smad2 Protein / Q000378:metabolism; D051900:Smad3 Protein / Q000378:metabolism; D051901:Smad4 Protein / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D015854:Up-Regulation
D000199:Actins; D018125:Receptors, Transforming Growth Factor beta; C497782:SMAD2 protein, human; C497790:SMAD3 protein, human; C497796:SMAD4 protein, human; D051785:Smad Proteins; D051899:Smad2 Protein; D051900:Smad3 Protein; D051901:Smad4 Protein; D016212:Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D030201:Activin Receptors, Type I; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II