Can Fourier transform infrared spectroscopy at higher wavenumbers (mid IR) shed light on biomarkers for carcinogenesis in tissues?
Fourier transform infrared microspectroscopy (FTIR-MSP) has shown promise as a technique for detection of abnormal cell proliferation and premalignant conditions. In the present study, we investigate the absorbance in the sensitive wavenumber region between 2800 and 3000 cm(-1), which has been known to be due to the antisymmetric and symmetric stretching vibrations of CH2 and CH3 groups of proteins and lipids. We report common biomarkers from this region that distinguish between normal and malignant tissues and cell lines. Based on our findings, we propose that the wavenumber region around 2800 to 3000 cm(-1) in the FTIR spectra of cells and tissues could provide valuable scientific evidence at the onset of premalignancy and may be used for ex vivo and in vitro detection of carcinogenesis. To further examine the utility of these markers in cancer diagnosis and management, they are tested successfully in monitoring the changes occurring in leukemia patients during chemotherapy.
Sahu
R K
RK
Ben Gurion University, Department of Physics and Cancer Research Center, Beer Sheva, Israel 84105.
Argov
S
S
Salman
A
A
Zelig
U
U
Huleihel
M
M
Grossman
N
N
Gopas
J
J
Kapelushnik
J
J
Mordechai
S
S
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D008055:Lipids / Q000032:analysis*; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis*; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D008055:Lipids; D009363:Neoplasm Proteins