Ursodeoxycholic acid differentially affects three types of sphingomyelinase in human colon cancer Caco 2 cells.
We investigated the effects of UDCA on sphingomyelinase (SMase) in Caco 2 cells cultured in monolayer and polarized conditions. Alkaline SMase activity was high in polarized cells whereas, acid and neutral SMase activities were high in monolayer cells. In polarized cells, UDCA increased alkaline SMase expression and caspase 3 activity but had no effect on acid and neutral SMases. In monolayer cells, UDCA reduced both acid and neutral SMase activities, inhibited cell proliferation, but had little effect on alkaline SMase and caspase 3 activities. In conclusion, UDCA differentially affects SMase activity, cell proliferation, and apoptosis in colonic cells depending on the cell conditions.
Liu
Fuli
F
Gastroenterology Lab, Biomedical Centre, B11, Lund University, S-22184 Lund, Sweden.
Cheng
Yajun
Y
Wu
Jun
J
Tauschel
Horst-Dietmar
HD
Duan
Rui-Dong
RD
D017209:Apoptosis / Q000187:drug effects*; D018938:Caco-2 Cells / Q000187:drug effects / Q000201:enzymology; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002756:Cholagogues and Choleretics / Q000494:pharmacology*; D006801:Humans; D013108:Sphingomyelin Phosphodiesterase / Q000187:drug effects / Q000378:metabolism*; D014580:Ursodeoxycholic Acid / Q000494:pharmacology*
D002756:Cholagogues and Choleretics; D014580:Ursodeoxycholic Acid; D013108:Sphingomyelin Phosphodiesterase; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases