[Effect of proto-oncogene protein c-met ribozyme transfection on biological behavior of gastric carcinoma cells].
OBJECTIVE
To explore the effect of c-met ribozyme transfection on biological behavior of gastric carcinoma cells.


METHODS
U1/met292 plasmid containing c-met ribozyme gene was transfected into L2 subline of SGC-7901 gastric cell line, and the proliferative ability, distribution of cell cycle, protein expression of VEGF and c-met, as well as the potential of liver metastasis of the transfected subline were determined.


RESULTS
There were no significant difference in proliferative ability, distribution of cell cycle between the transfected cells and the control cells. The protein expression of VEGF and c-met, as well as the liver metastatic potential significantly decreased in the transfected cells than those in the control cells (P< 0.05).


CONCLUSIONS
The liver metastatic potential of c-met positive gastric cancer cells may be prevented by inhibiting c-met expression.
Qin
Rong
R
Department of General Surgery, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China. rqin42@tom.com
He
Zhen-ping
ZP
D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D010957:Plasmids; D000090063:Proto-Oncogene Mas; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D019859:Proto-Oncogene Proteins c-met