title: Dietary PUFA modulate the expression of proliferation and differentiation markers in Morris 3924A hepatoma cells.
abstract: The effect of dietary polyunsaturated fatty acids on the expression of differentiation and proliferation markers in Morris 3924A hepatoma cells was investigated. ACT/I rats were conditioned 10 days with diets enriched with linoleic acid or alpha-linolenic acid before subcutaneous hepatoma cell transplantation. After 19 days from the inoculum, the mRNA levels of liver-enriched transcription factors and of their target genes were quantified. Both linoleic acid- and linolenic acid-enriched diets induced a decrease of beta-actin, AFP, PCNA, c-myc and of hepatocyte nuclear factors HNF-1alpha and HNF-4alpha mRNA levels in tumor tissue whereas HNF-3beta expression was induced by both dietary treatments. Only the alpha-linolenic acid-enriched diet was effective in reducing c-jun and increasing albumin mRNA levels. Since albumin is a C/EBPalpha target gene, C/EBPalpha gene transcription was evaluated at both protein and mRNA levels. It was found that alpha-linolenic acid-enriched diet did not enhance the C/EBPalpha mRNA content in hepatoma tissue while inducing C/EBPalpha protein expression with an isoform pattern similar to the hepatic phenotype. This evidence implies that alpha-linolenic acid or one of its metabolic products induce albumin synthesis in hepatoma cells by modulating C/EBPalpha gene expression at post-transcriptional level.
authors.affiliation: Department of Internal Medicine, Section of Biochemistry, University of Perugia, Via del Giochetto, 3, 06126 Perugia, Italy.
authors.forename: Alba
authors.initials: A
authors.lastname: Vecchini
authors.forename: Veronica
authors.initials: V
authors.lastname: Ceccarelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Nocentini
authors.forename: Carlo
authors.initials: C
authors.lastname: Riccardi
authors.forename: Paolo
authors.initials: P
authors.lastname: Di Nardo
authors.forename: Luciano
authors.initials: L
authors.lastname: Binaglia
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D005227:Fatty Acids / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage*; D015870:Gene Expression; D008055:Lipids / Q000737:chemistry; D008114:Liver Neoplasms, Experimental / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011912:Rats, Inbred ACI; D014157:Transcription Factors / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D022763:CCAAT-Enhancer-Binding Protein-alpha; D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014157:Transcription Factors