[Differentiation of neonatal rat striatal neural stem cells induced by all-trans retinoic acid].
OBJECTIVE
To investigate the role of all-trans retinoic acid (atRA) in inducing differentiation of neonatal rat striatal neural stem cells (NSCs).


METHODS
Neonatal rat striatal NSCs were obtained by mechanical isolation and serum-free culture. The roles of atRA at different concentrations in inducing the differentiation of NSCs were observed by immunofluorescent cytochemical staining, and the expression of retinoic acid receptor (RAR) gene was determined by semi-quantitative RT-PCR.


RESULTS
atRA could dose-dependently accelerate the differentiation of NSCs into neuron-like cells, and the physiological concentration of atRA was optimal for inducing NSC differentiation. atRA could induce the expression of RARbeta mRNA in a dose- and time-dependent manner.


CONCLUSIONS
atRA can accelerate differentiation of NSCs into neuron-likes cells and up-regulate the expression of RAR-beta mRNA in neonatal rat striatal NSCs.
Deng
Hong
H
College of Tropical Medicine, Southern Medical University, Guangzhou 510515, China. denghong-gzh@163.com
Zou
Fei
F
Luo
Hai-ji
HJ
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D018168:Receptors, Retinoic Acid / Q000096:biosynthesis / Q000235:genetics; D013234:Stem Cells / Q000166:cytology*; D014212:Tretinoin / Q000494:pharmacology*
D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D014212:Tretinoin