title: Human class-I restricted T cell associated molecule is highly expressed in the cerebellum and is a marker for activated NKT and CD8+ T lymphocytes.
abstract: We have previously reported the characterization of a novel immunoglobulin supergene family member, designated class-I MHC-restricted T cell associated molecule (CRTAM). Here we further characterize human CRTAM and find that it is highly expressed in the cerebellum, notably in Purkinje neurons. We identify CRTAM as a new member of the nectin-like (Necls) family and find significant expression of Necl-2 (IGSF4), a protein known to bind CRTAM and another member of the nectin superfamily, in the cerebellum. These findings suggest that CRTAM/Necl-2 binding may contribute to neuronal interactions. We also show that, in the immune system, CRTAM expression is restricted to activated class-I MHC-restricted T cells, including NKT and CD8 T cells. CRTAM represents one of the most highly expressed surface markers of activated human CD8 T cells and NKT cells, suggesting it may have diagnostic uses in various human viral and autoimmune diseases.
authors.affiliation: Department of Molecular Biomedicine, CINVESTAV Ave IPN # 2508 07360. Mexico D.F., 11340 Mexico.
authors.forename: Genaro
authors.initials: G
authors.lastname: Patiño-Lopez
authors.forename: Peter
authors.initials: P
authors.lastname: Hevezi
authors.forename: Jerry
authors.initials: J
authors.lastname: Lee
authors.forename: Dorian
authors.initials: D
authors.lastname: Willhite
authors.forename: Gail M
authors.initials: GM
authors.lastname: Verge
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Lechner
authors.forename: Vianney
authors.initials: V
authors.lastname: Ortiz-Navarrete
authors.forename: Albert
authors.initials: A
authors.lastname: Zlotnik
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000502:physiology; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D015759:Ionomycin / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008213:Lymphocyte Activation / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D027362:Organic Anion Transport Protein 1 / Q000378:metabolism*; D010802:Phylogeny; D011689:Purkinje Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment / Q000379:methods; D013997:Time Factors; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: C403514:CADM1 protein, human; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D008565:Membrane Proteins; D027362:Organic Anion Transport Protein 1; D012333:RNA, Messenger; C105033:Slc22a6 protein, mouse; D025521:Tumor Suppressor Proteins; C408522:class-I restricted T cell-associated molecule; D015759:Ionomycin