title: Comparison of three Legionella urinary antigen assays during an outbreak of legionellosis in Belgium.
abstract: During an outbreak of legionellosis in Belgium, urine samples of 32 legionellosis patients were tested with three Legionella urinary antigen assays: the Biotest enzyme immunoassay (EIA) kit, the Binax EIA kit and the Binax NOW Immunochromatographic Test kit. The three tests were concomitantly compared. The test sensitivities on the first urine samples were 65.6 % for the Biotest EIA, 50.0 % for the Binax EIA and 56.3 % for the Binax NOW. Testing of a second urine sample increased the sensitivities to 71.9 %, 59.4 % and 65.6 %, respectively. The differences were not statistically significant. In outbreak settings, testing second samples from patients presenting with symptoms but initially testing negative and/or concentrating urine samples for testing might be valuable additions to the urinary antigen test to increase the sensitivities of the tests.
authors.affiliation: Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
authors.forename: Kristien
authors.initials: K
authors.lastname: Dirven
authors.affiliation: Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
authors.forename: Margareta
authors.initials: M
authors.lastname: Ieven
authors.affiliation: Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
authors.forename: Marcel F
authors.initials: MF
authors.lastname: Peeters
authors.affiliation: Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
authors.forename: Anneke
authors.initials: A
authors.lastname: van der Zee
authors.affiliation: Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
authors.forename: Koen
authors.initials: K
authors.lastname: De Schrijver
authors.affiliation: Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
authors.forename: Herman
authors.initials: H
authors.lastname: Goossens
mesh_terms: D000942:Antigens, Bacterial / Q000652:urine*; D001530:Belgium / Q000453:epidemiology; D004196:Disease Outbreaks; D006801:Humans; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D007875:Legionella / Q000276:immunology* / Q000302:isolation & purification*; D007876:Legionellosis / Q000175:diagnosis / Q000453:epidemiology* / Q000652:urine; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial; D011933:Reagent Kits, Diagnostic