title: 2-D reference map of Bacillus anthracis vaccine strain A16R proteins.
abstract: Bacillus anthracis has always been an important pathogen because it can cause lethal inhalational anthrax, and may be used as a bioweapon or by bioterrorists. In this study, a 2-DE reference map and database of B. anthracis A16R was constructed. In total, 534 spots were processed, and 406 spots representing 299 proteins were identified. Gel-estimated pIs and molecular masses mostly matched well with their theoretical predictions, but some discrepancies also existed. Spot and protein corresponding analysis revealed that post-translational modifications might be common in B. anthracis. Through the MASCOT search, the similarity of B. anthracis, B. cereus and B. thuringiensis was further verified by protein level and a possible annotation error in B. anthracis strain Ames 0581 genome was found. Proteins of energy metabolism, fatty acid and phospholipid metabolism, protein synthesis, and cellular processes represented a large part of the most abundant proteins. At the same time, 27 hypothetical proteins were experimentally proved. There were 28 proteins also identified as spore composition in recently spore-related research, which indicated that they might play some roles in different phases such as growth, sporulation and outgrowth. Maps and information about all identified proteins are available on the Internet at http://www.mpiib-berlin.mpg.de/2D-PAGE and http://www.proteomics.com.cn.
authors.affiliation: State Key Laboratory of Pathogens and Biosecurity, Beijing, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Wang
authors.forename: Tianyi
authors.initials: T
authors.lastname: Ying
authors.forename: Hengliang
authors.initials: H
authors.lastname: Wang
authors.forename: Zhaoxing
authors.initials: Z
authors.lastname: Shi
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: He
authors.forename: Erling
authors.initials: E
authors.lastname: Feng
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Kaihua
authors.initials: K
authors.lastname: Wei
authors.forename: Guofu
authors.initials: G
authors.lastname: Su
authors.forename: Houchu
authors.initials: H
authors.lastname: Zhu
authors.forename: Xuemin
authors.initials: X
authors.lastname: Zhang
authors.forename: Peitang
authors.initials: P
authors.lastname: Huang
authors.forename: Liuyu
authors.initials: L
authors.lastname: Huang
mesh_terms: D022122:Anthrax Vaccines / Q000737:chemistry / Q000302:isolation & purification*; D001408:Bacillus anthracis / Q000276:immunology* / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013171:Spores, Bacterial / Q000737:chemistry / Q000276:immunology
chemical_list: D022122:Anthrax Vaccines; D001426:Bacterial Proteins