title: Genomic structure and promoter activity of the E1AF gene, a member of the ETS oncogene family.
abstract: E1AF is a member of the ETS oncogene family and is thought to be a human homologue of mouse PEA3. We have isolated a genomic clone of E1AF and analyzed the promoter activity of its 5'-flanking region. We identified a variation in exon 1, which depends on the cell type. There was no typical TATA box in the 5'-flanking region, but putative binding sites of a number of transcription factors including PEA3 as well as CAAT boxes were seen. A luciferase reporter assay indicated that the 5'-flanking region possesses promoter activity. Northern blot studies demonstrated significant expression of the E1AF gene in restricted tissues such as the pituitary gland, placenta, and fetal kidney. Moreover, the E1AF promoter was activated by E1AF itself and estrogen receptor. These findings suggest that E1AF is a housekeeping gene, whose expression is controlled in specific tissues.
authors.affiliation: Department of Microbiology, Hokkaido Institute of Public Health, N-19, W-12, Kita-ku, Sapporo 060-0819, Japan.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Ishida
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Higashino
authors.forename: Mariko
authors.initials: M
authors.lastname: Aoyagi
authors.forename: Akiko
authors.initials: A
authors.lastname: Takahashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Masanobu
authors.initials: M
authors.lastname: Shindoh
authors.forename: Kei
authors.initials: K
authors.lastname: Fujinaga
authors.forename: Koichi
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D024506:5' Flanking Region; D017500:Adenovirus E1A Proteins / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D005091:Exons; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D050783:Proto-Oncogene Proteins c-ets; D011960:Receptors, Estrogen / Q000502:physiology; D016385:TATA Box
chemical_list: D017500:Adenovirus E1A Proteins; C079853:ETV4 protein, human; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D011960:Receptors, Estrogen; D008156:Luciferases