title: Sphingomonas yunnanensis sp. nov., a novel gram-negative bacterium from a contaminated plate.
abstract: A Gram-negative bacterium, YIM 003T, which was isolated from a contaminated plate in the laboratory, was subjected to a polyphasic taxonomic study. The organism had short-rod-shaped, motile cells, formed yellow-pigmented colonies on ISP2 medium and its optimum growth pH was 7.0-7.5. The major respiratory lipoquinone was ubiquinone Q-10. The phosphate-containing lipids detected in strain YIM 003T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and one unidentified phospholipid. The major fatty acids were C(18 : 1)omega7c (59.8 %), C(16 : 0) (9.9 %), ai-C(17 : 0) (5.3 %), i-C(17 : 0) (4.4 %) and C(14 : 0) 2-OH (15.8 %). The G+C content of the genomic DNA was 67.5 mol%. Strain YIM 003T exhibited levels of 16S rRNA gene sequence similarity of 98.2 % to Sphingomonas phyllosphaerae FA2T and 98.0 % to Sphingomonas adhaesiva DSM 7418T but showed less than 97.0 % similarity with respect to other species with validly published names. The DNA-DNA relatedness values of the isolate with S. phyllosphaerae FA2T and S. adhaesiva DSM 7418T were 59 and 26 %, respectively. The phenotypic characteristics and genotypic data indicate that strain YIM 003T should be distinguished from S. phyllosphaerae FA2T and S. adhaesiva DSM 7418T. Therefore, on the basis of the polyphasic taxonomic data presented, a novel species of the genus Sphingomonas, Sphingomonas yunnanensis sp. nov., is proposed, with the type strain YIM 003T (=CCTCC AB 204064T=KCTC 12346T).
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Yi-Guang
authors.initials: YG
authors.lastname: Chen
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Xin-Peng
authors.initials: XP
authors.lastname: Tian
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Jiang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D020459:Genes, rRNA; D005840:Gentian Violet; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010619:Phenazines; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D020578:Sphingomonas / Q000145:classification / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D005227:Fatty Acids; C035863:Gram's stain; D010619:Phenazines; D012336:RNA, Ribosomal, 16S; D005840:Gentian Violet