Molecular cloning and expression of human keratinocyte proline-rich protein (hKPRP), an epidermal marker isolated from calcium-induced differentiating keratinocytes.
We isolated a human gene encoding keratinocyte proline-rich protein (hKPRP). hKPRP gene is located in the region of epidermal differentiation complex on chromosome 1q21, and its approximately 2.5 kb mRNA encodes 579 amino acid protein with high proline content (18%). The mRNA level of hKPRP was markedly increased at both 7 and 14 d after treatment with 1.2 mM calcium in cultured normal human epidermal keratinocytes. In situ hybridization demonstrated that hKPRP was expressed in upper granular layer of normal epidermis with characteristic intermittent pattern. In psoriatic lesion, hKPRP expression was increased as compared with normal skin and showed continuous pattern. Immunohistochemical analysis also confirmed the expression of hKPRP at the protein level. Western blot analysis showed that hKPRP protein of approximately 70 kDa size was significantly increased by calcium in a time-dependent manner. In mouse tissue blot assays, the expression of KPRP was detected in stomach and skin tissues, and began at 17.5 embryonic days. Additionally, hKPRP expression was detected in the periderm of human fetal skin from 16 wk estimated gestational age. Together, these results suggest that hKPRP is an epidermal marker expressed in stratified squamous epithelia and has a potential role in keratinocytes differentiation.
Lee
Woong-Hee
WH
Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Korea.
Jang
Sunhyae
S
Lee
Jung-Suk
JS
Lee
Young
Y
Seo
Eun-Young
EY
You
Kwan-Hee
KH
Lee
Seung-Chul
SC
Nam
Kwang-Il
KI
Kim
Jin-Man
JM
Kee
Sun-Ho
SH
Yang
Jun-Mo
JM
Seo
Young-Joon
YJ
Park
Jang-Kyu
JK
Kim
Chang Deok
CD
Lee
Jeung-Hoon
JH
D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002118:Calcium / Q000494:pharmacology; D002454:Cell Differentiation; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D003001:Cloning, Molecular; D004622:Embryo, Mammalian / Q000378:metabolism; D000078404:Epidermal Cells; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D011565:Psoriasis / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism
D015415:Biomarkers; C476447:KPRP protein, human; D011506:Proteins; D012333:RNA, Messenger; D002118:Calcium