[Expression of human ERMAP gene in different cell lines].
To investigate the pattern of human ERMAP gene expression in different cell lines, 15 cell lines derived from hematopoietic tumor, somatic tumor and normal tissue were chosen and were cultured; cells were harvested after culture for 12, 24, 36, 48, 60 and 72 hours; the expression of the human ERMAP was detected by using fluorescent quantitative PCR. The results showed that human ERMAP gene expression was positive in K562 cell line at interval of 12 and 24 hours, and the expression levels were (5.092 +/- 2.331) x 10(6) cps/microl RNA, (5.328 +/- 3.916) x 10(6) cps/microl RNA, respectively; ERMAP gene expression was also positive in ECV304 cell line at interval of 24 hours, and the expression level was (0.84 +/- 0.12) x 10(6) cps/microl RNA; and its expression was negative in other 13 cell lines. It is concluded that human ERMAP gene expression in ECV304 cell line was found first, and its expression in K562 cell line was further confirmed.
He
Ying-Yi
YY
Department of Pediatrics, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.
Zhang
Xiao-Hong
XH
Ye
Tie-Zhen
TZ
Wu
Zi-Liang
ZL
D001789:Blood Group Antigens / Q000235:genetics*; D000071797:Butyrophilins; D002460:Cell Line; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D019337:Hematologic Neoplasms / Q000235:genetics / Q000473:pathology; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D001789:Blood Group Antigens; D000071797:Butyrophilins; D012333:RNA, Messenger; C472715:scianna antigens