title: Detection of homocysteine and cysteine.
abstract: At elevated levels, homocysteine (Hcy, 1) is a risk factor for cardiovascular diseases, Alzheimer's disease, neural tube defects, and osteoporosis. Both 1 and cysteine (Cys, 3) are linked to neurotoxicity. The biochemical mechanisms by which 1 and 3 are involved in disease states are relatively unclear. Herein, we describe simple methods for detecting either Hcy or Cys in the visible spectral region with the highest selectivity reported to date without using biochemical techniques or preparative separations. Simple methods and readily available reagents allow for the detection of Cys and Hcy in the range of their physiologically relevant levels. New HPLC postcolumn detection methods for biological thiols are reported. The potential biomedical relevance of the chemical mechanisms involved in the detection of 1 is described.
authors.affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
authors.forename: Weihua
authors.initials: W
authors.lastname: Wang
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Rusin
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Xu
authors.forename: Kyu Kwang
authors.initials: KK
authors.lastname: Kim
authors.forename: Jorge O
authors.initials: JO
authors.lastname: Escobedo
authors.forename: Sayo O
authors.initials: SO
authors.lastname: Fakayode
authors.forename: Kristin A
authors.initials: KA
authors.lastname: Fletcher
authors.forename: Mark
authors.initials: M
authors.lastname: Lowry
authors.forename: Corin M
authors.initials: CM
authors.lastname: Schowalter
authors.forename: Candace M
authors.initials: CM
authors.lastname: Lawrence
authors.forename: Frank R
authors.initials: FR
authors.lastname: Fronczek
authors.forename: Isiah M
authors.initials: IM
authors.lastname: Warner
authors.forename: Robert M
authors.initials: RM
authors.lastname: Strongin
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D003545:Cysteine / Q000097:blood* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006710:Homocysteine / Q000097:blood* / Q000737:chemistry; D006801:Humans; D010455:Peptides / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D028561:Transition Elements / Q000737:chemistry
chemical_list: D000447:Aldehydes; D005456:Fluorescent Dyes; D010455:Peptides; D013438:Sulfhydryl Compounds; D013844:Thiazoles; D028561:Transition Elements; D006710:Homocysteine; D003545:Cysteine