[Expression and significance of the matrix metalloproteinase inhibitor RECK gene in prostate cell strains].
OBJECTIVE
To investigate the expression and significance of RECK gene and MMP-9 in prostate cell strains such as BPH-1, DU45, LNCaP and PC-3.


METHODS
The expression of mRNA of RECK and MMP-9 was measured by RT-PCR and RECK protein was evaluated by Western blot.


RESULTS
The mRNA level of the RECK gene in the prostate carcinoma cell strains, such as DU45, LNCaP and PC-3, was lower than that in the benign prostate hyperplasia cell line BPH-1, while MMP-9 had a higher expression. The protein level of RECK in DU-45, LNCaP and PC-3 was lower than that in the BPH-1.


CONCLUSIONS
The RECK gene is supposed to be a kind of tumor suppressor gene, which may act by inhibiting the activity of MMP-9.
Xu
Zhen-yu
ZY
Department of Urology, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China. dr_zhenyu1976@yahoo.com.cn
Gao
Jian-ping
JP
Zhang
Zheng-pu
ZP
Ge
Jing-ping
JP
Xu
Chuan-liang
CL
Sun
Ying-hao
YH
D015153:Blotting, Western; D045744:Cell Line, Tumor; D058851:GPI-Linked Proteins; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; C115460:RECK protein, human; D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9