title: Genetic mapping of quantitative trait loci affecting susceptibility in chicken to develop pulmonary hypertension syndrome.
abstract: Pulmonary hypertension syndrome (PHS), also referred to as ascites syndrome, is a growth-related disorder of chickens frequently observed in fast-growing broilers with insufficient pulmonary vascular capacity at low temperature and/or at high altitude. A cross between two genetically different broiler dam lines that originated from the White Plymouth Rock breed was used to produce a three-generation population. This population was used for the detection and localization of quantitative trait loci (QTL) affecting PHS-related traits. Ten full-sib families consisting of 456 G2 birds were typed with 420 microsatellite markers covering 24 autosomal chromosomes. Phenotypic observations were collected on 4202 G3 birds and a full-sib across family regression interval mapping approach was used to identify QTL. There was statistical evidence for QTL on chicken chromosome 2 (GGA2), GGA4 and GGA6. Suggestive QTL were found on chromosomes 5, 8, 10, 27 and 28. The most significant QTL were located on GGA2 for right and total ventricular weight as percentage of body weight (%RV and %TV respectively). A related trait, the ratio of right ventricular weight as percentage to total ventricular weight (RATIO), reached the suggestive threshold on this chromosome. All three QTL effects identified on GGA2 had their maximum test statistic in the region flanked by markers MCW0185 and MCW0245 (335-421 cM).
authors.affiliation: Animal Breeding and Genetics Group, Wageningen Institute of Animal Sciences, Wageningen, The Netherlands.
authors.forename: T S K M
authors.initials: TS
authors.lastname: Rabie
authors.forename: R P M A
authors.initials: RP
authors.lastname: Crooijmans
authors.forename: H
authors.initials: H
authors.lastname: Bovenhuis
authors.forename: A L J
authors.initials: AL
authors.lastname: Vereijken
authors.forename: T
authors.initials: T
authors.lastname: Veenendaal
authors.forename: J J
authors.initials: JJ
authors.lastname: van der Poel
authors.forename: J A M
authors.initials: JA
authors.lastname: Van Arendonk
authors.forename: A
authors.initials: A
authors.lastname: Pakdel
authors.forename: M A M
authors.initials: MA
authors.lastname: Groenen
mesh_terms: D000818:Animals; D002645:Chickens; D002874:Chromosome Mapping / Q000662:veterinary*; D003433:Crosses, Genetic; D020022:Genetic Predisposition to Disease; D006976:Hypertension, Pulmonary / Q000235:genetics / Q000662:veterinary*; D018895:Microsatellite Repeats / Q000235:genetics; D010641:Phenotype; D011201:Poultry Diseases / Q000235:genetics*; D040641:Quantitative Trait Loci