title: Protective effect of a radical scavenger, MCI-186 on islet cell damages induced by oxidative stress.
abstract: Oxidative stress generated during islet isolation and transplantation causes islet cell damage. These oxidative injuries are mediated by reactive oxygen species (ROS) and reactive nitrogen species (RNS). MCI-186 is an antioxidant used for clinical treatment of cerebral infarction in Japan. We examined a possible protective effect of MCI-186 on islet cells against oxidative stress. Islets isolated from Sprague-Dawley rats by collagenase P digestion were purified by density gradient centrifugation with Ficoll. Islets were treated with hydrogen peroxide (H(2)O(2); 5 to 250 micromol) in the presence or absence of MCI-186. Cell death was measured by an LDH release assay. Maximum islet cell death was observed at 250 micromol of H(2)O(2). MCI-186 inhibited islet cell death in a dose-dependent manner with significant reduction above 30 micromol. From the results observed we suggest that the antioxidant effects of MCI-186 may prove beneficial to improve the preservation of islet cells.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery and Regeneration Technology, Faculty of Medicine, Kochi University, Kochi 783-8505, Japan.
authors.forename: P
authors.initials: P
authors.lastname: Rao
authors.forename: H
authors.initials: H
authors.lastname: Maeda
authors.forename: X
authors.initials: X
authors.lastname: Yutong
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Hirose
authors.forename: S
authors.initials: S
authors.lastname: Sasaguri
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide; D000077553:Edaravone; D000983:Antipyrine