Investigation on the gastroprotective and antidiarrhoeal properties of Aegle marmelos unripe fruit extract.
The Study was designed to verify the gastroprotective and antidiarrhoeal effects of unripe fruit extract of Aegle marmelos Corr. The gastroprotective function of this extract was evaluated in rats against gastric mucosal damage induced by hypothermic restraint stress, absolute ethanol, and indomethacin, whereas the antidiarrhoeal activity was investigated by studying the influence on gastrointestinal transit as measured by a charcoal marker and on castor oil-induced accumulation of intestinal fluid in mice and also on contractile responses evoked by acetylcholine, histamine, serotonin, and barium chloride in isolated guinea-pig ileum, the results demonstrated that pretreatment of animals with unripe fruit extract (50 and 100 mg/kg, i.p.) produces a significant inhibition of gastric lesion induced by ethanol but not those induced by restraint stress or indomethacin and suggest a probable involvement of a prostaglandin-independent mechanism of gastroprotection. At similar doses, both the intestinal transit as well as the accumulation of intestinal fluids induced by castor oil in mice were significantly inhibited by raw fruit extract. Furthermore, the extract antagonized the contractile responses evoked by different agonists on guinea-pig ileum in vitro and its inhibitory potential for the drugs are in the order of acetylcholine > histamine > serotonin > barium chloride. Taken together, these results point out a possible antidiarrhoeal effect of unripe fruit extract of A. marmelos Corr., since inhibition of intestinal motility and secretion can control clinical diarrhoea.
Dhuley
J N
JN
Pharmacology & Toxicology section, Research Centre, Hindustan Antibiotics Limited, Pimpri, Pune 411 018, India.
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000930:Antidiarrheals / Q000302:isolation & purification / Q000494:pharmacology*; D002492:Central Nervous System Depressants; D000431:Ethanol; D005638:Fruit / Q000737:chemistry; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007213:Indomethacin; D008297:Male; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D010936:Plant Extracts / Q000627:therapeutic use; D051381:Rats; D027821:Rutaceae / Q000737:chemistry*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000209:etiology / Q000517:prevention & control; D013315:Stress, Psychological / Q000150:complications
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D000930:Antidiarrheals; D002492:Central Nervous System Depressants; D010936:Plant Extracts; D000431:Ethanol; D007213:Indomethacin