In vivo detection of scrapie cases from blood by infrared spectroscopy.
In the present study, infrared spectroscopy was shown to be able to distinguish healthy and scrapie-infected animals by analysis of the white-cell membranous fraction from blood. Infrared spectroscopy was able to detect not only clinical cases, but also animals at a preclinical stage of the disease. These findings suggest this technique as an accurate in vivo diagnostic tool that could be applied to animal as well as human samples. In addition to possibly avoiding the slaughter of a huge number of animals with the socio-economic consequences that this poses, the test could be expected to become useful in the prevention of human transmission by blood transfusion.
Carmona
Pedro
P
Instituto de Estructura de la Materia (CSIC), Serrano 121, 28006 Madrid, Spain.
Monzón
Marta
M
Centro Nacional de Referencia de EET (University of Zaragoza), Miguel Servet 177, 50013 Zaragoza, Spain.
Monleón
Eva
E
Centro Nacional de Referencia de EET (University of Zaragoza), Miguel Servet 177, 50013 Zaragoza, Spain.
Badiola
Juan José
JJ
Centro Nacional de Referencia de EET (University of Zaragoza), Miguel Servet 177, 50013 Zaragoza, Spain.
Monreal
Jaime
J
Instituto de Neurobiología Santiago Ramón y Cajal (CSIC), Doctor Arce 37, 28006 Madrid, Spain.
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D007962:Leukocytes / Q000737:chemistry; D012608:Scrapie / Q000097:blood* / Q000175:diagnosis*; D012756:Sheep; D017550:Spectroscopy, Fourier Transform Infrared