SUMO keeps a check on recombination during DNA replication.
The small ubiquitin-related modifier SUMO plays an important role in the maintenance of genome stability. Accordingly, DNA replication, repair and recombination factors as well as mediators of chromosome dynamics and cohesion are among its many targets. Attachment of SUMO can modulate the properties of the modified proteins by affecting localization, conformation, stability or enzymatic activity, but often its mechanism of action remains poorly defined. Recent findings demonstrate how SUMO modification of PCNA, the processivity clamp for replicative DNA polymerases, prevents unscheduled recombination during DNA replication by means of directly enhancing physical interactions with an anti-recombinogenic helicase, Srs2. This review highlights how the SUMO conjugation system exerts its effect on the replication fork and discusses the implications for ubiquitin-dependent DNA damage tolerance.
Ulrich
Helle D
HD
Cancer Research UK, Clare Hall Laboratories, South Mimms, United Kingdom. helle.ulrich@cancer.org.uk
Vogel
Sabina
S
Davies
Adelina A
AA
D000818:Animals; D004265:DNA Helicases / Q000378:metabolism; D004261:DNA Replication / Q000235:genetics*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics*; D016196:S Phase; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*
D018809:Proliferating Cell Nuclear Antigen; D029701:Saccharomyces cerevisiae Proteins; D025841:Small Ubiquitin-Related Modifier Proteins; C061376:SRS2 protein, S cerevisiae; D004265:DNA Helicases