Correlation between Musashi-1 and c-hairy-1 expression and cell proliferation activity in the developing intestine and stomach of both chicken and mouse.
Musashi-1 (Msi-1) is an RNA-binding protein that plays key roles in the maintenance of neural stem cell states and in their differentiation into neural cells. Msi-1 has also been proposed as a candidate marker gene of mammalian intestinal stem cells and their immediate lineages. In this study, we examined Msi-1 expression in the small intestine and the stomach of both chicken and mouse during embryonic, fetal and postnatal development. In addition, we analyzed the expression of c-hairy-1, a chicken homologue of mouse Hes1, and assessed the proliferative activity of the cells expressing both of these factors. Significantly, during the development of these digestive organs in both species Msi-1 expression showed dynamic changes, suggesting that it is important for digestive organ development, particularly for epithelial differentiation. Based on our observations of the expression patterns of Msi-1 and c-hairy-1 in the adult small intestine, we speculate that Msi-1 is also a stem cell marker of the chicken small intestinal epithelium.
Asai
Rieko
R
Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1-1, Minamiosawa, Hachioji, Tokyo 192-0397, Japan.
Okano
Hideyuki
H
Yasugi
Sadao
S
D000818:Animals; D030161:Avian Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D002642:Chick Embryo; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D007421:Intestine, Small / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D011531:Proventriculus / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000502:physiology; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D013270:Stomach / Q000166:cytology* / Q000196:embryology*; D000072056:Transcription Factor HES-1
D030161:Avian Proteins; D051792:Basic Helix-Loop-Helix Proteins; D018797:Cell Cycle Proteins; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; D000072056:Transcription Factor HES-1; C489734:Hes1 protein, mouse; C104786:Msi1h protein, mouse; C109424:hairy1 protein, Gallus gallus