title: Effects of riboflavin on the phototransformation of benzo[a]pyrene.
abstract: Riboflavin (Vitamin B2) is a natural dye-sensitizer habitually present in natural waters. Effects of riboflavin as photosensitizer on the transformation of benzo[a]pyrene (BaP) (10 microM) in the aqueous-organic solvent (water/acetonitrile/methanol 50/40/10) were investigated in this study. The photolysis half life of BaP in solution containing 50 microM riboflavin was 5 min, compared to 98 min in the absence of riboflavin. The rate of phototransformation of BaP increased as the concentration of riboflavin was raised from 10 microM to 100 microM under both natural sunlight and UVA irradiation. The half life of BaP in the presence of 50 microM riboflavin was 10.6 min and 43.1 min when exposed to visible range of natural sunlight and UVA irradiation respectively. Riboflavin decomposes under natural sunlight. Lumichrome, a principal photoproduct of riboflavin, was shown to photosensitize BaP under natural sunlight after photolysis of riboflavin. Our study indicated that other photoproducts from riboflavin, such as lumiflavin, were also involved in the phototransformation of BaP under sunlight when riboflavin diminished. The major photoproducts in the photolysis of BaP were identified as 1,6-benzo[a]pyrene-dione, 3,6-benzo[a]pyrene-dione, 6,12-benzo[a]pyrene-dione by using high performance liquid chromatography (HPLC). All these products were detected in the samples which were irradiated under different light sources and in the presence or absence of riboflavin. The possible phototransformation mechanism was discussed.
authors.affiliation: Department of Biology, Jackson State University, 1400 Lynch Street, Box 18540, Jackson, MS 39217, USA.
authors.forename: Xueheng
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Hu
authors.forename: Huey-Min
authors.initials: HM
authors.lastname: Hwang
mesh_terms: D001564:Benzo(a)pyrene / Q000032:analysis* / Q000528:radiation effects; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000737:chemistry*; D012256:Riboflavin / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000528:radiation effects
chemical_list: D017319:Photosensitizing Agents; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D012256:Riboflavin