title: Microtubule-associated protein 8 contains two microtubule binding sites.
abstract: Microtubule-associated proteins (MAPs) are critical regulators of microtubule dynamics and functions, and have long been proposed to be essential for many cellular events including neuronal morphogenesis and functional maintenance. In this study, we report the characterization of a new microtubule-associated protein, we named MAP8. The protein of MAP8 is mainly restricted to the nervous system postnatally in mouse. Its expression could first be detected as early as at embryonic day 10, levels plateau during late embryonic and neonatal periods, and subsequently decrease moderately to remain constant into adulthood. In addition to its carboxyl terminal binding site, the MAP8 polyprotein also contains a functional microtubule-binding domain at its N-terminal segment. The association of the carboxyl terminal of the light chain with actin microfilaments could also be detected. Our findings define MAP8 as a novel microtubule associated protein containing two microtubule binding domains.
authors.affiliation: Department of Neurology and Neurological Sciences, Stanford University School of Medicine, 1201 Welch Road, Stanford, CA 94305-5489, USA.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Ding
authors.forename: Angela
authors.initials: A
authors.lastname: Valle
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Allen
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Timothy
authors.initials: T
authors.lastname: Nardine
authors.forename: Yingjiu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lily
authors.initials: L
authors.lastname: Peng
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Yang
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001665:Binding Sites; D019556:COS Cells; D002522:Chlorocebus aethiops; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D009420:Nervous System / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D009928:Organ Specificity; D017434:Protein Structure, Tertiary
chemical_list: D008869:Microtubule-Associated Proteins