Involvement of oxidative stress in hydroquinone-induced cytotoxicity in catalase-deficient Escherichia coli mutants.
Hydroquinone is a benzene-derived metabolite. To clarify whether the reactive oxygen species (ROS) are involved in hydroquinone-induced cytotoxicity, we constructed transformants of Escherichia coli (E. coli) strains that express mammalian catalase gene derived from catalase mutant mice (Cs(b), Cs(c)) and the wild-type (Cs(a)) using a catalase-deficient E. coli UM255 as a recipient. Specific catalase activities of these tester strains were in order of Cs(a) > Cs(c) > Cs(b) > UM255, and their susceptibility to hydrogen peroxide (H2O2) showed UM255 > Cs(b) > Cs(c) > Cs(a). We found that hydroquinone exposure reduced the survival of catalase-deficient E. coli mutants in a dose-dependent manner significantly, especially in the strains with lower catalase activities. Hydroquinone toxicity was also confirmed using zone of inhibition test, in which UM255 was the most susceptible, showing the largest zone of growth inhibition, followed by Cs(b), Cs(c) and Cs(a). Furthermore, we found that hydroquinone-induced cell damage was inhibited by the pretreatment of catalase, ascorbic acid, dimethyl sulfoxide (DMSO), and ethylenediaminetetraacetic acid (EDTA), and augmented by superoxide dismutase (both CuZnSOD and MnSOD). The present results suggest that H2O2 is probably involved in hydroquinone-induced cytotoxicity in catalase-deficient E. coli mutants and catalase plays an important role in protection of the cells against hydroquinone toxicity.
Horita
Masako
M
Okayama University Graduate School of Medicine and Dentistry, Department of Public Health, Okayama, Japan.
Wang
Da-Hong
DH
Tsutsui
Ken
K
Sano
Kuniaki
K
Masuoka
Noriyoshi
N
Kira
Shohei
S
D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006873:Hydroquinones / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D015394:Molecular Structure; D009154:Mutation / Q000235:genetics; D018384:Oxidative Stress
D000975:Antioxidants; D006873:Hydroquinones; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D002374:Catalase; C031927:hydroquinone