title: [Purification of the effective component from Solanum xanthocarpum and its effect against Oncomelania snails].
abstract: OBJECTIVE
  To extract and purify the active component from Solanum for Oncomelania snail control, evaluate its effect against the snails and determine its chemical structure.
  
  
  METHODS
  Pedicel and seed were moved and the remaining part of the Solanum xanthocarpum fruit was torrefied and ground into powder. The powder was soaked into 95% ethanol and extracted. The residue was treated by ethyl acetate. 7 components were separated by methods of thin layer chromatography (TLC) and column chromatography (CCL). 4 main components were observed for the effect of snail-killing by immersion method. An alkaloid with Rf = 0. 58, named as component A, was confirmed as one of the effective components for Oncomelania snail control. The component A was further purified and received Al and A2 by proper elusion, and their effect was re-tested. The molecular weight and chemical structure of A2 were determined by MS, NMR and IR respectively.
  
  
  RESULTS
  The death rate of Oncomelania snails was 94.2% when the concentration of component A was 2.50 mg/L1(28 C ). The flow liquid system (ethyl acetate: chloroform: methanol 11:11:35) applied was the best reagent for separating component A. The death rate of Oncomelania snails in solution of A2 (0.2 mg/L) was 100% (28 degrees C). The molecular weight of A2 was 867 with an mp 298-305 C.
  
  
  CONCLUSIONS
  The effective agent (A2),one of the active components from the fruit of Solanum xanthocar pum, is alpha-solamargine which shows an excellent effect in killing Oncomelania snails.
authors.affiliation: Tongji Medical College, Huazhong university of Science and Technology, Wuhan 430032, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Li
authors.forename: Xi
authors.initials: X
authors.lastname: Cheng
authors.forename: Chun-jing
authors.initials: CJ
authors.lastname: Wang
authors.forename: Gui-ling
authors.initials: GL
authors.lastname: Li
authors.forename: Shu-zhen
authors.initials: SZ
authors.lastname: Xia
authors.forename: Feng-hua
authors.initials: FH
authors.lastname: Wei
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015394:Molecular Structure; D008975:Molluscacides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D012908:Snails / Q000187:drug effects* / Q000254:growth & development; D032322:Solanum / Q000737:chemistry*
chemical_list: D006027:Glycosides; D008975:Molluscacides; D010840:Phytosterols; D010936:Plant Extracts