Cryo-electron microscopy reveals conserved and divergent features of gag packing in immature particles of Rous sarcoma virus and human immunodeficiency virus.
Retrovirus assembly proceeds via multimerisation of the major structural protein, Gag, into a tightly packed, spherical particle that buds from the membrane of the host cell. The lateral packing arrangement of the human immunodeficiency virus type 1 (HIV-1) Gag CA (capsid) domain in the immature virus has been described. Here we have used cryo-electron microscopy (cryo-EM) and image processing to determine the lateral and radial arrangement of Gag in in vivo and in vitro assembled Rous sarcoma virus (RSV) particles and to compare these features with those of HIV-1. We found that the lateral packing arrangement in the vicinity of the inner sub-domain of CA is conserved between these retroviruses. The curvature of the lattice, however, is different. RSV Gag protein adopts a more tightly curved lattice than is seen in HIV-1, and the virions therefore contain fewer copies of Gag. In addition, consideration of the relationship between the radial position of different Gag domains and their lateral spacings in particles of different diameters, suggests that the N-terminal MA (matrix) domain does not form a single, regular lattice in immature retrovirus particles.
Briggs
John A G
JA
Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7BN, UK.
Johnson
Marc C
MC
Simon
Martha N
MN
Fuller
Stephen D
SD
Vogt
Volker M
VM
D001358:Avian Sarcoma Viruses / Q000737:chemistry* / Q000502:physiology; D020285:Cryoelectron Microscopy; D005583:Fourier Analysis; D015683:Gene Products, gag / Q000096:biosynthesis / Q000737:chemistry*; D006678:HIV / Q000737:chemistry* / Q000502:physiology; D012190:Retroviridae; D014771:Virion / Q000737:chemistry*; D019065:Virus Assembly
D015683:Gene Products, gag