title: Structure of a ribulose 5-phosphate 3-epimerase from Plasmodium falciparum.
abstract: The crystal structure of Pfal009167AAA, a putative ribulose 5-phosphate 3-epimerase (PfalRPE) from Plasmodium falciparum, has been determined to 2 A resolution. RPE represents an exciting potential drug target for developing antimalarials because it is involved in the shikimate and the pentose phosphate pathways. The structure is a classic TIM-barrel fold. A coordinated Zn ion and a bound sulfate ion in the active site of the enzyme allow for a greater understanding of the mechanism of action of this enzyme. This structure is solved in the framework of the Structural Genomics of Pathogenic Protozoa (SGPP) consortium.
authors.affiliation: SLAC, Stanford University, Menlo Park, California, USA.
authors.forename: J
authors.initials: J
authors.lastname: Caruthers
authors.forename: J
authors.initials: J
authors.lastname: Bosch
authors.forename: F
authors.initials: F
authors.lastname: Buckner
authors.forename: W
authors.initials: W
authors.lastname: Van Voorhis
authors.forename: P
authors.initials: P
authors.lastname: Myler
authors.forename: E
authors.initials: E
authors.lastname: Worthey
authors.forename: C
authors.initials: C
authors.lastname: Mehlin
authors.forename: E
authors.initials: E
authors.lastname: Boni
authors.forename: G
authors.initials: G
authors.lastname: DeTitta
authors.forename: J
authors.initials: J
authors.lastname: Luft
authors.forename: A
authors.initials: A
authors.lastname: Lauricella
authors.forename: O
authors.initials: O
authors.lastname: Kalyuzhniy
authors.forename: L
authors.initials: L
authors.lastname: Anderson
authors.forename: F
authors.initials: F
authors.lastname: Zucker
authors.forename: M
authors.initials: M
authors.lastname: Soltis
authors.forename: Wim G J
authors.initials: WG
authors.lastname: Hol
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000138:chemical synthesis; D001665:Binding Sites; D002238:Carbohydrate Epimerases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D015195:Drug Design; D008958:Models, Molecular; D010963:Plasmodium falciparum / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017510:Protein Folding; D017433:Protein Structure, Secondary; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012542:Scattering, Radiation; D013499:Surface Properties; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D000962:Antimalarials; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D002238:Carbohydrate Epimerases; C022553:ribulosephosphate 3-epimerase