BnNHL18A shows a localization change by stress-inducing chemical treatments.
The two genes, named BnNHL18A and BnNHL18B, showing sequence homology with Arabidopsis NDR1/HIN1-like (NHL) genes, were isolated from cDNA library prepared with oilseed rape (Brassica napus) seedlings treated with NaCl. The transcript level of BnNHL18A was increased by sodium chloride, ethephon, hydrogen peroxide, methyl jasmonate, or salicylic acid treatment. The coding regions of BnNHL18A and BnNHL18B contain a sarcolipin (SLN)-like sequence. Analysis of the localization of smGFP fusion proteins showed that BnNHL18A is mainly localized to endoplasmic reticulum (ER). This result suggests that the SLN-like sequence plays a role in retaining proteins in ER membrane in plants. In response to NaCl, hydrogen peroxide, ethephon, and salicylic acid treatments, the protein localization of BnNHL18A was changed. Our findings suggest a common function of BnNHL18A in biotic and abiotic stresses, and demonstrate the presence of the shared mechanism of protein translocalization between the responses to plant pathogen and to osmotic stress.
Lee
Suk-Bae
SB
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Ham
Byung-Kook
BK
Park
Jeong Mee
JM
Kim
Young Jin
YJ
Paek
Kyung-Hee
KH
D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics; D029688:Brassica napus / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics; D009943:Organophosphorus Compounds / Q000494:pharmacology; D009997:Osmotic Pressure; D054883:Oxylipins; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D011510:Proteolipids / Q000235:genetics; D020156:Salicylic Acid / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics
D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D009124:Muscle Proteins; C109369:NDR1 protein, Arabidopsis; D009943:Organophosphorus Compounds; D054883:Oxylipins; D010940:Plant Proteins; D011510:Proteolipids; D014157:Transcription Factors; C077258:sarcolipin; D012965:Sodium Chloride; C072239:methyl jasmonate; D006861:Hydrogen Peroxide; D020156:Salicylic Acid; C005073:ethephon