Cathepsin L cysteine proteinase in the diagnosis of bovine Fasciola gigantica infection.
Cathepsin L cysteine proteinase from Fasciola gigantica was evaluated for its potential in the early prepatent detection of this helminth infection in bovine calves. Five cross-bred bovine calves were experimentally infected with 400 metacercariae/calf and evaluated for anti-cathepsin L antibody response. F. gigantica infection in these calves could be detected 4 weeks post-infection using an ELISA, dipstick ELISA and Western blotting with 100% sensitivity. The antigen was also used to detect F. gigantica field infection in cattle, by screening 256 sera of these animals by an ELISA, which demonstrated an overall infection rate of 26.95%. Preliminary studies showed that F. gigantica cathepsin L cysteine proteinase does not cross-react with Paramphistomum epiclitum, Gigantocotyle explanatum and hydatid cyst antigens. However, extensive studies on the cross-reactivity of this antigen with related helminth parasites of cattle and buffaloes are required, before this antigen can be considered suitable for immuno-diagnosis of fasciolosis in these ruminants.
Sriveny
D
D
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, India.
Raina
O K
OK
Yadav
S C
SC
Chandra
D
D
Jayraw
A K
AK
Singh
M
M
Velusamy
R
R
Singh
B P
BP
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western / Q000662:veterinary; D002403:Cathepsins / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003429:Cross Reactions; D003546:Cysteine Endopeptidases / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005209:Fasciola / Q000201:enzymology* / Q000276:immunology; D005211:Fascioliasis / Q000175:diagnosis / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D015801:Helminth Proteins / Q000276:immunology* / Q000302:isolation & purification; D007159:Immunologic Tests / Q000662:veterinary; D011897:Random Allocation; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D002403:Cathepsins; C086102:cat-L1B protein, Fasciola gigantica; D003546:Cysteine Endopeptidases