title: Periodontal therapy: a novel acute inflammatory model.
abstract: OBJECTIVE
  We designed a clinical trial at the Eastman Dental Hospital with the objective of developing a novel in vivo inflammatory model.
  
  
  METHODS
  We recruited 55 subjects suffering from severe periodontitis.
  
  
  METHODS
  Participants received intensive periodontal therapy.
  
  
  METHODS
  Blood samples were collected at baseline and 1 and 7 and 30 days after treatment and processed for a series of biomarkers (TNF-alpha, IL-6, CRP and Fibrinogen) by high-sensitivity assays and differential blood counts (standard laboratory procedures).
  
  
  RESULTS
  TNF-alpha levels were significantly raised only after 1 day of therapy (P<0.01) whereas IL-6 (P<0.01), CRP (P<0.001) and Fibrinogen (P<0.001) concentrations peaked 24 hrs after and returned to baseline values within one month following therapy. Mild neutrophilia, monocytosis and lymphopenia were also observed.
  
  
  CONCLUSIONS
  Intensive periodontal therapy induces a one week moderate inflammatory response and therefore it is proposed as a novel therapeutic and reliable non drug-induced in vivo model of acute inflammation.
authors.affiliation: Periodontology Unit, Eastman Dental Institute and Hospital, University College London, 256 Gray's Inn Road, London WC1X 8LD, UK. f.daiuto@eastman.ucl.ac.uk
authors.forename: F
authors.initials: F
authors.lastname: D'Aiuto
authors.forename: M
authors.initials: M
authors.lastname: Parkar
authors.forename: M S
authors.initials: MS
authors.lastname: Tonetti
mesh_terms: D000208:Acute Disease; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D018448:Models, Immunological; D010518:Periodontitis / Q000097:blood / Q000276:immunology / Q000473:pathology* / Q000628:therapy*