OP-142 gene expression profile of nonfunctional human pancreatic islets: predictors of transplant failure?
Islet culture has become a standard part of most successful protocols for clinical islet transplantation. To date, however, islets are transplanted based on crude measures of viability, purity and in vitro insulin production without adequate prior assessment of the potential for in vivo function. The purpose of this study was to define the gene expression profiles of human islets associated with in vivo function using a nonimmune NOD-scid mouse model. Human islets from eight isolations were maintained in culture for 7 to 14 days in Memphis serum-free media until transplanted. The RNA was extracted from 10,000 IEQ using RNASTAT-60. The gene expression profiles were analyzed using high-density Affymetrix U133A GeneChips and Genespring software. An aliquot of 2000 IEQ from each islet preparation was also transplanted into NOD-scid animals (n = 5) for in vivo function assessments. Islet function was assessed by measurements of human C-peptide at days 7 and 14 posttransplant. Human C-peptide levels were determined by radioimmunoassay. Gene analysis of nonfunction islets (4 of 8 islet preparations) showed high relative levels of expression of proinflammatory genes and low relative levels of genes directed toward insulin processing and secretion as well as islet integrity. Overexpression of hypoxia and proinflammatory genes may result in reduced insulin secretion and lead to islet destruction posttransplantation. Identifying and validating those genes could allow the development of a potency assay for human transplantation that would be very useful for screening human islet preparations before clinical transplant.
Sabek
O M
OM
Department of Surgery, University of Tennessee Health Science Center, 956 Court Avenue, Memphis, TN 38103, USA. osabek@utmem.edu
Marshall
D R
DR
Minoru
O
O
Fraga
D W
DW
Gaber
A O
AO
D000328:Adult; D000818:Animals; D002096:C-Peptide / Q000097:blood*; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000235:genetics; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000502:physiology*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D002096:C-Peptide; D018975:Insulin-Like Growth Factor Binding Protein 5; D042461:Vascular Endothelial Growth Factor A