title: Expressed protein ligation to obtain selectively modified aldo/keto reductases.
abstract: Specific enzyme immobilization has moved into the focus for many applications in biochemical research fields. Expressed Protein Ligation (EPL) has been proven to be ideal to selectively label proteins at single positions. Applying this technique to enzymes of the aldo/keto reductase superfamily provides a new approach to generate native or modified redox enzymes for direct and indirect immobilization.
authors.affiliation: Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Brüderstrasse 34, D-04103 Leipzig, Germany.
authors.forename: Michael P O
authors.initials: MP
authors.lastname: Richter
authors.forename: Annette G
authors.initials: AG
authors.lastname: Beck-Sickinger
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000648:ultrastructure; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D000818:Animals; D002384:Catalysis; D018360:Crystallography, X-Ray; D004800:Enzymes, Immobilized / Q000737:chemistry; D006801:Humans; D011506:Proteins / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D011506:Proteins; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase