title: [Confirmation of non-permitted dyes detected in Akasu (red vinegar) by LC/MS].
abstract: Suitable liquid chromatography/mass spetrometry (LC/MS) conditions were examined for Amaranth, Red 2G (R2G), Azo Rubine (Azo), Fast Red E (FRE) and Brilliant Blue FCF, which were detected in Akasu, a red vinegar made in Hong Kong from sake lees, on both thin layer chromatography (TLC) and photodiode array high-performance liquid chromatography (PDA-HPLC). Molecular-related ions for each dye were detected with excellent sensitivity by LC/MS using electro-spray ionization with negative ion mode, capillary voltage 3.00 kV, cone voltage 50 V and desolvation temperature 400 degrees C. LC/MS analysis of refined Akasu under these conditions enabled us to obtain clear mass spectra of R2G, Azo and FRE, which were present at trace levels in the Akasu. The results were consistent with those from TLC and PDA-HPLC. These experiments suggested that LC/MS analysis is applicable for confirmation of dyes in food.
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo, Japan.
authors.forename: Fusako
authors.initials: F
authors.lastname: Ishikawa
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Oishi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Shindo
authors.forename: Masao
authors.initials: M
authors.lastname: Horie
authors.forename: Akiko
authors.initials: A
authors.lastname: Yasui
authors.forename: Shuzo
authors.initials: S
authors.lastname: Ogino
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
mesh_terms: D019342:Acetic Acid / Q000032:analysis*; D000548:Amaranth Dye / Q000032:analysis; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D004396:Coloring Agents / Q000032:analysis*; D013058:Mass Spectrometry
chemical_list: D004396:Coloring Agents; D000548:Amaranth Dye; D019342:Acetic Acid