The role of SMG-1 in nonsense-mediated mRNA decay.
SMG-1, a member of the PIKK (phosphoinositide 3-kinase related kinases) family, plays a critical role in the mRNA quality control system termed nonsense-mediated mRNA decay (NMD). NMD protects the cells from the accumulation of aberrant mRNAs with premature termination codons (PTCs) that encode nonfunctional or potentially harmful truncated proteins. SMG-1 directly phosphorylates Upf1, another key component of NMD, and this phosphorylation occurs upon recognition of PTC on post-spliced mRNA during the initial round of translation. At present, a variety of tools are available that can specifically suppress NMD, and it is possible to examine the contribution of NMD in a variety of physiological and pathological conditions.
Yamashita
Akio
A
Department of Molecular Biology, Yokohama City University School of Medicine and Graduate School of Medical Science, Kanazawa-ku, Yokohama 236-0004, Japan.
Kashima
Isao
I
Ohno
Shigeo
S
D000818:Animals; D018389:Codon, Nonsense / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008954:Models, Biological; D008957:Models, Genetic; D010455:Peptides / Q000235:genetics; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010766:Phosphorylation; D020365:RNA Helicases; D020871:RNA Stability / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism
D018389:Codon, Nonsense; D010455:Peptides; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D020365:RNA Helicases; D012333:RNA, Messenger; D015534:Trans-Activators; C101828:UPF1 protein, human