Determination of celecoxib in human plasma and breast milk by high-performance liquid chromatographic assay.
A rapid and simple HPLC assay was developed for the determination of celecoxib in human plasma and breast milk. After proteins were precipitated with acetonitrile, celecoxib was resolved on a C18 column and detected by UV detection at 254 nm. Standard curves were linear over the concentration range 10-2000 microg/L (r(2)>0.99). Bias was </=+/-15% from 20 to 2000 microg/L in both matrices, intra- and inter-day coefficients of variation (imprecision) were <10%, and the limit of quantification was 10 microg/L.
Zhang
Mei
M
Clinical Pharmacology, Department of Medicine, Christchurch School of Medicine and Health Sciences, University of Otago, Christchurch, New Zealand. mei.zhang@cdhb.govt.nz
Moore
Grant A
GA
Gardiner
Sharon J
SJ
Begg
Evan J
EJ
D000068579:Celecoxib; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D011720:Pyrazoles / Q000097:blood* / Q000592:standards; D012015:Reference Standards; D015203:Reproducibility of Results; D013449:Sulfonamides / Q000097:blood* / Q000592:standards
D011720:Pyrazoles; D013449:Sulfonamides; D000068579:Celecoxib