The protective effect of L-carnitine in peripheral blood human lymphocytes exposed to oxidative agents.
Literature data indicate L-carnitine (LC), a trans-mitochondrial carrier of acetyl and long chain groups, as an agent possessing protective effects against oxidative stress in mammalian cells. However, the major factor involved in the protective mechanism is not known. The protection activity exerted by this agent against reactive oxygen species induced by hydrogen peroxide (H2O2) and t-butylhydroperoxide (t-butyl-OOH) treatment in isolated human peripheral blood lymphocytes (PBLs) has been studied. Human lymphocytes cells were isolated and pre-incubated with 5 mM LC before H2O2 (100 microM) and t-butyl-OOH (400 microM) treatment. The protective effect of LC on treated PBLs was measured by single cell gel electrophoresis and the analysis of chromosomal aberrations. Results show that lc treated cells exhibited a significant decrease in the number of oxidative induced single-strand breaks and chromosomal aberrations.
Garcia
Claudia Lorenti
CL
Laboratory of Molecular Cytogenetic and Mutagenesis, DABAC, Università degli Studi della Tuscia, Via S.Camillo de Lellis, I-01100 Viterbo, Italy.
Filippi
Silvia
S
Mosesso
Pasquale
P
Calvani
Menotti
M
Nicolai
Raffaella
R
Mosconi
Luigi
L
Palitti
Fabrizio
F
D002331:Carnitine / Q000494:pharmacology*; D002869:Chromosome Aberrations / Q000187:drug effects*; D020552:Comet Assay; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects*; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014803:Vitamin B Complex / Q000494:pharmacology*; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D016877:Oxidants; D020011:Protective Agents; D017382:Reactive Oxygen Species; D014803:Vitamin B Complex; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D002331:Carnitine