[Cloning of beta-tubulin gene from Gibberella zeae and analysis its relationship with carbendazim-resistance].
Whole beta-tubulin genes from wild carbendazim(MBC)-sensitive isolate, field MBC-resistant isolate and induced MBC-resistant mutant of Gibberella zeae were cloned and sequenced with 3 pairs of primers. These genes have 1631bp length, including 3 introns, encoding 447 amino acid. The homology of amino acid tubulin gene of G. zeae with that of other common plant pathogenic filamentous fungi is from 95.12% approximately 99.30%. Sequence comparison among MBCs, field MBCR and induced MBCR isolates revealed there was no mutation, even one. So it can be concluded that the mechanism of MBC-resistance to G. zeae is different from other filamentous fungi caused by point mutation at amino acid position 198 or other position of beta-tubulin gene.
Li
Hongxia
H
Key Laboratory of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture, Nanjing Agriculture University, Nanjing 210095, China. hxli2002@yahoo.com.cn
Lu
Yuejian
Y
Wang
Jianxin
J
Zhou
Mingguo
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D001562:Benzimidazoles / Q000494:pharmacology*; D002219:Carbamates / Q000494:pharmacology*; D003001:Cloning, Molecular; D025141:Drug Resistance, Fungal; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005874:Gibberella / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014404:Tubulin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D001562:Benzimidazoles; D002219:Carbamates; D005656:Fungal Proteins; D014404:Tubulin; C006698:carbendazim