A 12,000-rad porcine radiation hybrid (IMNpRH2) panel refines the conserved synteny between SSC12 and HSA17.
Reverse or bidirectional Zoo-FISH suggests that synteny between porcine chromosome 12 (SSC12) and human chromosome 17 (HSA17) is completely conserved. The construction of a high-resolution radiation hybrid (RH) map for SSC12 provides a unique opportunity to determine whether chromosomal synteny is reflected at the molecular level by comparative gene mapping of SSC12 and HSA17. We report an initial, high-resolution RH map of SSC12 on the 12,000-rad IMNpRH2 panel using CarthaGene software. This map contains a total of 320 markers, including 20 microsatellites and 300 ESTs/genes, covering approximately 4836.9 cR12,000. The markers were ordered in 16 linkage groups at LOD 6.0 using framework markers previously mapped on the IMpRH7000-rad SSC12 and porcine genetic maps. Ten linkage groups ordered more than 10 markers, with the largest containing 101 STSs. The resolution of the current RH map is approximately 15.3 kb/cR on SSC12, a significant improvement over the second-generation EST SSC12 RH7000-rad map of 103 ESTs and 15 framework markers covering approximately 2287.2 cR7000. Compared to HSA17, six distinct segments were identified, revealing macro-rearrangements within the apparently complete synteny between SSC12 and HSA17. Further analysis of the order of 245 genes (ESTs) on HSA17 and SSC12 also revealed several micro-rearrangements within a synteny segment. A high-resolution SSC12 RH12,000-rad map will be useful in fine-mapping QTL and as a scaffold for sequencing this chromosome.
Liu
Wan-Sheng
WS
Department of Animal Biotechnology, College of Agriculture, Biotechnology, and Natural Resources, University of Nevada at Reno, Mail Stop 202, 1664 N. Virginia Street, Reno, NV 89557, USA. wsliu@cabnr.unr.edu
Eyer
Katie
K
Yasue
Hiroshi
H
Roelofs
Benjamin
B
Hiraiwa
Hideki
H
Shimogiri
Takeshi
T
Landrito
Earl
E
Ekstrand
Joseph
J
Treat
Michael
M
Rink
Anette
A
Yerle
Martine
M
Milan
Denis
D
Beattie
Craig W
CW
D000818:Animals; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D020224:Expressed Sequence Tags; D006801:Humans; D008126:Lod Score; D018895:Microsatellite Repeats / Q000235:genetics; D021101:Radiation Hybrid Mapping; D034421:Sus scrofa / Q000235:genetics*; D026801:Synteny / Q000235:genetics*