title: Characterisation of a new splice variant of MASK-BP3(ARF) and MASK human genes, and their expression patterns during haematopoietic cell differentiation.
abstract: In this study we report the characterisation of a new splice variant, here denominated splice variant 4 (accession number AF258557) of the human Multiple Ankyrin repeats Single KH domain (hMASK) (accession number AF521882) and the hMASK-4E-Binding Protein 3 Alternative Reading Frame (hMASK-BP3(ARF)) (accession number AF521883), containing a number of ANK-repeat motifs. Ankyrin (ANK) repeat-containing proteins carry out a wide variety of biological activities and are involved in processes, such as cell differentiation and transcriptional regulation. The present study reports the computer analysis of these splice variant cDNAs and their broad mRNA expression in different normal human tissues and cancer cell lines. An upregulation of the splice variant mRNAs expression was observed after HL-60 and erythroblast differentiation. The upregulation of splice variant 4 mRNA was considerably higher than those of the other variants, during erythroid differentiation.
authors.affiliation: Centro de Hematologia e Hemoterapia, Universidade Estadual de Campinas, Campinas, SP 13083-970, Brazil.
authors.forename: Adriana da Silva
authors.initials: Ada S
authors.lastname: Santos Duarte
authors.forename: Fabíola
authors.initials: F
authors.lastname: Traina
authors.forename: Patricia M Bergamo
authors.initials: PM
authors.lastname: Favaro
authors.forename: Daniela Sanchez
authors.initials: DS
authors.lastname: Bassères
authors.forename: Iara Campos
authors.initials: IC
authors.lastname: de Carvalho
authors.forename: Samuel de Souza
authors.initials: Sde S
authors.lastname: Medina
authors.forename: Fernando Ferreira
authors.initials: FF
authors.lastname: Costa
authors.forename: Sara Teresinha Olalla
authors.initials: ST
authors.lastname: Saad
mesh_terms: D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D017931:DNA Primers; D015870:Gene Expression; D006801:Humans; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012326:RNA Splicing; D000099317:Hippo Kinases
chemical_list: D017931:DNA Primers; D017346:Protein Serine-Threonine Kinases; C430908:STK26 protein, human; D000099317:Hippo Kinases