title: Interaction between HIF-1 alpha (ODD) and hARD1 does not induce acetylation and destabilization of HIF-1 alpha.
abstract: Hypoxia inducible factor-1 alpha (HIF-1 alpha) is a central component of the cellular responses to hypoxia. Hypoxic conditions result in stabilization of HIF-1 alpha and formation of the transcriptionally active HIF-1 complex. It was suggested that mammalian ARD1 acetylates HIF-1 alpha and thereby enhances HIF-1 alpha ubiquitination and degradation. Furthermore, ARD1 was proposed to be down-regulated in hypoxia thus facilitating the stabilization of HIF-1 alpha. Here we demonstrate that the level of human ARD1 (hARD1) protein is not decreased in hypoxia. Moreover, hARD1 does not acetylate and destabilize HIF-1 alpha. However, we find that hARD1 specifically binds HIF-1 alpha, suggesting a putative, still unclear, connection between these proteins.
authors.affiliation: Department of Molecular Biology, University of Bergen, N-5020 Bergen, Norway.
authors.forename: Thomas
authors.initials: T
authors.lastname: Arnesen
authors.forename: Xianguo
authors.initials: X
authors.lastname: Kong
authors.forename: Rune
authors.initials: R
authors.lastname: Evjenth
authors.forename: Darina
authors.initials: D
authors.lastname: Gromyko
authors.forename: Jan Erik
authors.initials: JE
authors.lastname: Varhaug
authors.forename: Zhao
authors.initials: Z
authors.lastname: Lin
authors.forename: Nianli
authors.initials: N
authors.lastname: Sang
authors.forename: Jaime
authors.initials: J
authors.lastname: Caro
authors.forename: Johan R
authors.initials: JR
authors.lastname: Lillehaug
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000693:Anaerobiosis; D002478:Cells, Cultured; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D063209:N-Terminal Acetyltransferase A; D063213:N-Terminal Acetyltransferase E
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D000123:Acetyltransferases; D063209:N-Terminal Acetyltransferase A; C504824:NAA10 protein, human; D063213:N-Terminal Acetyltransferase E