Effect of tea catechins on mitochondrial DNA 4977-bp deletions in human leucocytes.
The catechins in green tea have antioxidative and antimutagenic effects. We examined the effect of green tea enriched with catechins on the presence of mitochondrial DNA (mtDNA) with a common 4977-bp deletion mutation (mtDNA4977) in human leucocytes. Ten healthy females [aged 20.80 +/- 1.03 years] drank 350 ml of catechin-rich tea daily after supper for 5 weeks. Blood samples were collected twice before, and twice after 5 weeks of consuming the tea. Deletions in mtDNA were analyzed using the nested polymerase chain reaction (PCR). We identified a common mtDNA4977 deletion in nine participants before drinking the tea. However, this mtDNA4977 deletion was not evident in leucocytes from most of the participants 5 weeks after drinking the tea. Catechins found in tea might contribute to the maintenance of health status by reducing damage to mtDNA and by maintaining the capacity of mtDNA for oxidative phosphorylation.
Iwai
Koichi
K
Center for Humanity and Sciences, Ibaraki Prefectural University of Health Sciences, 4669-2, Ami, Inashiki, Ibaraki 300-0394, Japan. iwai@ipu.ac.jp
Iwamura
Yukio
Y
Yamashita
Shuhei
S
Wadano
Yasuyoshi
Y
Mesaki
Noboru
N
D000328:Adult; D020029:Base Pairing / Q000235:genetics*; D002392:Catechin / Q000494:pharmacology*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D017384:Sequence Deletion / Q000187:drug effects* / Q000235:genetics; D013662:Tea / Q000737:chemistry*
D004272:DNA, Mitochondrial; D013662:Tea; D002392:Catechin