title: Detecting and aligning peaks in mass spectrometry data with applications to MALDI.
abstract: In this paper, we address the peak detection and alignment problem in the analysis of mass spectrometry data. To deal with the peak redundancy problem existing in the MALDI data acquired in the reflectron mode, we propose to use the amplitude modulation technique in peak detection. The alignment of two peak sets is formulated as a non-rigid registration problem and is solved using a robust point matching (RPM) approach. To align multiple peak sets, we first use a super set method to find a common peak set among all peak sets as a standard and then align all peak sets to the standard using the robust point matching approach in a sequential manner (i.e. We align only one peak set to the standard each time, thus reducing the multiple peak set alignment problem to a simpler two peak set alignment problem). Experimental results from a study of ovarian cancer data set show that the quantitative cross-correlation coefficients among technical replicates are increased after peak alignment. Additional comparisons also demonstrate that our method has a similar performance as the hierarchical clustering method, although the implementations of these methods are different.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, Suite 503, 300 George Street, New Haven, CT 06520, USA. weichuan.yu@yale.edu
authors.forename: Weichuan
authors.initials: W
authors.lastname: Yu
authors.forename: Baolin
authors.initials: B
authors.lastname: Wu
authors.forename: Ning
authors.initials: N
authors.lastname: Lin
authors.forename: Kathy
authors.initials: K
authors.lastname: Stone
authors.forename: Kenneth
authors.initials: K
authors.lastname: Williams
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhao
mesh_terms: D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010051:Ovarian Neoplasms / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010455:Peptides