Chiral separations on multichannel microfluidic chips.
Chiral separations of FITC-labeled basic drugs on multichannel microfluidic chips with LIF detector were investigated. A preliminary screening procedure for seven neutral CDs was performed under optimized conditions for chiral separations of three FITC-labeled drugs (baclofen, norfenefrine, and tocainide) on a mono-channel microfluidic chip. According to the results of screening, FITC-baclofen and FITC-norfenefrine as well as two chiral selectors including gamma-CD and dimethyl-beta-CD (DM-beta-CD) were selected as models to perform chiral separations on a two-channel chip. FITC-baclofen enantiomers were separated completely by gamma-CD in one channel, while resolution of FITC-norfenefrine enantiomers was achieved by DM-beta-CD in the other channel in the same run. Furthermore, the feasibility of using one chiral selector to separate multiple chiral samples was studied on a four-channel chip. These results show that multichannel chip has a potential for chiral high-throughput screening.
Gao
Yan
Y
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
Shen
Zheng
Z
Wang
Hui
H
Dai
Zhongpeng
Z
Lin
Bingcheng
B
D001418:Baclofen / Q000031:analogs & derivatives / Q000302:isolation & purification; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D009655:Octopamine / Q000031:analogs & derivatives / Q000302:isolation & purification; D013237:Stereoisomerism; D016677:Tocainide / Q000031:analogs & derivatives / Q000302:isolation & purification; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins
D005456:Fluorescent Dyes; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; D009655:Octopamine; D016677:Tocainide; C038119:heptakis(2,6-O-dimethyl)beta-cyclodextrin; C005310:norfenefrine; D001418:Baclofen; D016650:Fluorescein-5-isothiocyanate