title: [Inter-laboratory study for validation of a Japanese official analytical method for determination of patulin in apple juice].
abstract: To validate a modified version of AOAC official method of analysis 995.10 as an official standard in Japan for determination of patulin in apple juice, an inter-laboratory study was performed in 11 laboratories using a non-contaminated sample, 2 naturally contaminated samples and 2 spiked samples of apple juice. For naturally contaminated apple juices, the relative standard deviations for repeatability and reproducibility were 3.2, 7.1% and 10.0, 21.7%, respectively. HORRAT values were 0.4, 0.9. The average recovery of patulin from spiked sample was 83.7%. The limit of quantification was calculated as 10 microg/kg. From these results, the method was thought to be suitable as an official standard for determination of patulin in apple juice in Japan.
authors.affiliation: National Institute of Health Sciences, Setagaya-ku, Tokyo, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Sugita-Konsihi
authors.forename: Toshitugu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Yoshitugu
authors.initials: Y
authors.lastname: Sugiura
authors.forename: Setsuko
authors.initials: S
authors.lastname: Tabata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakajima
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakurai
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakaie
authors.forename: Koichi
authors.initials: K
authors.lastname: Sato
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kitani
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Fujita
authors.forename: Sachiko
authors.initials: S
authors.lastname: Hayashi
authors.forename: Tayoshi
authors.initials: T
authors.lastname: Iizuka
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Hirakawa
authors.forename: Naoki
authors.initials: N
authors.lastname: Mochizuki
authors.forename: Mariko
authors.initials: M
authors.lastname: Hoshino
authors.forename: Yoshirou
authors.initials: Y
authors.lastname: Sato
authors.forename: Naomi
authors.initials: N
authors.lastname: Takahashi
authors.forename: Kosuke
authors.initials: K
authors.lastname: Takatori
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000592:standards; D007564:Japan; D027845:Malus / Q000737:chemistry*; D010365:Patulin / Q000032:analysis*
chemical_list: D010365:Patulin