title: Ubiquitination of APOBEC3 proteins by the Vif-Cullin5-ElonginB-ElonginC complex.
abstract: APOBEC3 proteins are antiviral host factors for a wide variety of retroviruses. HIV-1 Vif overcomes the antiviral activity of APOBEC3G by ubiquitinating the protein. In this study, we examined the ability of Vif to antagonize other family members of APOBEC3 proteins, together with its mechanism. Using HIV infectivity, virion incorporation, immunoprecipitation, and in vitro ubiquitin conjugation assays, we show that the ability of Vif to inhibit antiviral activity of APOBEC3 proteins positively correlates with its ability to bind and ubiquitinate these proteins by a Vif-Cullin5-ElonginB-ElonginC (Vif-BC-Cul5) complex. These results suggest that Vif exhibits its anti-APOBEC3 activity by the ubiquitin ligase activity of the Vif-BC-Cul5 complex.
authors.affiliation: Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Shirakawa
authors.forename: Akifumi
authors.initials: A
authors.lastname: Takaori-Kondo
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Tomonaga
authors.forename: Taisuke
authors.initials: T
authors.lastname: Izumi
authors.forename: Keiko
authors.initials: K
authors.lastname: Fukunaga
authors.forename: Amane
authors.initials: A
authors.lastname: Sasada
authors.forename: Aierken
authors.initials: A
authors.lastname: Abudu
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Miyauchi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Akari
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Iwai
authors.forename: Takashi
authors.initials: T
authors.lastname: Uchiyama
mesh_terms: D000071480:APOBEC-3G Deaminase; D002460:Cell Line; D044842:Cullin Proteins / Q000737:chemistry / Q000378:metabolism*; D003564:Cytidine Deaminase / Q000737:chemistry / Q000378:metabolism; D043525:Cytosine Deaminase / Q000737:chemistry / Q000378:metabolism; D000075065:Elongin; D015967:Gene Expression Regulation, Viral; D016346:Gene Products, vif / Q000737:chemistry / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism; D006801:Humans; D015778:Minor Histocompatibility Antigens; D009700:Nucleoside Deaminases / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism*; D054320:vif Gene Products, Human Immunodeficiency Virus
chemical_list: D044842:Cullin Proteins; D000075065:Elongin; D016346:Gene Products, vif; D015778:Minor Histocompatibility Antigens; D012097:Repressor Proteins; D014157:Transcription Factors; D025801:Ubiquitin; D054320:vif Gene Products, Human Immunodeficiency Virus; D009700:Nucleoside Deaminases; C492906:APOBEC3F protein, human; D043525:Cytosine Deaminase; D000071480:APOBEC-3G Deaminase; C495794:APOBEC3B protein, human; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase