title: Phylogenetic analysis of the gag region encoding the matrix protein of small ruminant lentiviruses: comparative analysis and molecular epidemiological applications.
abstract: Little sequence information exists on the matrix-protein (MA) encoding region of small ruminant lentiviruses (SRLV). Fifty-two novel sequences were established and permitted a first phylogenetic analysis of this region of the SRLV genome. The variability of the MA encoding region is higher compared to the gag region encoding the capsid protein and surprisingly close to that reported for the env gene. In contrast to primate lentiviruses, the deduced amino acid sequences of the N- and C-terminal domains of MA are variable. This permitted to pinpoint a basic domain in the N-terminal domain that is conserved in all lentiviruses and likely to play an important functional role. Additionally, a seven amino acid insertion was detected in all MVV strains, which may be used to differentiate CAEV and MVV isolates. A molecular epidemiology analysis based on these sequences indicates that the Italian lentivirus strains are closely related to each other and to the CAEV-CO strain, a prototypic strain isolated three decades ago in the US. This suggests a common origin of the SRLV circulating in the monitored flocks, possibly related to the introduction of infected goats in a negative population. Finally, this study shows that the MA region is suitable for phylogenetic studies and may be applied to monitor SRLV eradication programs.
authors.affiliation: Department of Veterinary Pathology, Hygiene and Public Health, University of Milano, via Celoria 10, 20133 Milano, Italy. giuliano.pisoni@unimi.it
authors.forename: Giuliano
authors.initials: G
authors.lastname: Pisoni
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Bertoni
authors.forename: Paul
authors.initials: P
authors.lastname: Boettcher
authors.forename: Wilma
authors.initials: W
authors.lastname: Ponti
authors.forename: Paolo
authors.initials: P
authors.lastname: Moroni
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D017124:Conserved Sequence; D015683:Gene Products, gag / Q000235:genetics*; D006041:Goats; D007558:Italy; D016086:Lentivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016180:Lentivirus Infections / Q000662:veterinary* / Q000821:virology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014763:Viral Matrix Proteins / Q000235:genetics*
chemical_list: D015683:Gene Products, gag; D012367:RNA, Viral; D014763:Viral Matrix Proteins