Up-regulation of the claudin-6 gene in adipogenesis.
To investigate the role of claudin-6 in adipogenesis, claudin-6 mRNA was examined in adipose tissues and adipocyte differentiation. Claudin-6 mRNA was found to be differentially expressed in four different adipose tissues, and up-regulated in each fat depot of mice fed a high-fat diet as compared to a normal-fat diet. Levels of claudin-6 transcripts were increased during differentiation of 3T3-L1 cells in vitro. Moreover, small interfering RNA (siRNA)-mediated reduction of claudin-6 mRNA inhibited differentiation of 3T3-L1 cells. These results suggest that claudin-6 is another important regulator in adipogenesis and fat deposition.
Hong
Yeon-Hee
YH
Department of Food Production Science, Faculty of Agriculture, Shinshu University, Nagano, Japan.
Hishikawa
Daisuke
D
Miyahara
Hisae
H
Nishimura
Yukihiko
Y
Tsuzuki
Hiroaki
H
Gotoh
Chizu
C
Iga
Tomoyo
T
Suzuki
Yasuki
Y
Song
Sang-Houn
SH
Choi
Ki-Choon
KC
Lee
Hong-Gu
HG
Sasaki
Shinichi
S
Roh
Sang-Gun
SG
D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000235:genetics*; D000273:Adipose Tissue / Q000737:chemistry / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D057167:Claudins; D004041:Dietary Fats / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000494:pharmacology; D015854:Up-Regulation / Q000235:genetics*
D057167:Claudins; D004041:Dietary Fats; D008565:Membrane Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C122101:claudin 6