[Effects of yiqi huayu recipe and its decomposed formulas on apoptosis-related factors of anulus fibrosus cells in rats].
OBJECTIVE
To study the effects of Yiqi Huayu Recipe and its decomposed formulas-medicated sera on expressions of bcl-2, Bax and caspase-8 of apoptotic anulus fibrosus cells in rats.


METHODS
Immunohistochemical and integral optical density analytic methods were used to observe the effects of Yiqi Huayu Recipe-, Yiqi Recipe-, Huayu Recipe-medicated sera and insulin-like growth factor 1 (IGF-1) on the expressions of bcl-2, Bax and caspase-8 of apoptotic anulus fibrosus cells in rats induced by anti-Fas antibody.


RESULTS
As compared with apoptosis group, bcl-2 expression was higher, Bax and caspase-8 expressions were lower in Yiqi Huayu-treated, Yiqi-treated, Huayu-treated and IGF-1 groups (P<0.01). As compared with Yiqi-treated group and Huayu-treated group, Bax expression was lower in Yiqi Huayu-treated group (P<0.05).


CONCLUSIONS
Yiqi Huayu Recipe and its decomposed formulas can delay degeneration of the cervical intervertebral disc, which may be due to its action in regulating the expressions of bcl-2, Bax and caspase-8.
Zhou
Quan
Q
Spinal Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Wang
Yong-Jun
YJ
Shi
Qi
Q
Sun
Peng
P
Zhou
Chong-Jian
CJ
Hu
Zhi-Jun
ZJ
Liu
Mei
M
D000818:Animals; D000831:Animals, Newborn; D053181:Caspase 8; D020169:Caspases / Q000096:biosynthesis / Q000235:genetics; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007403:Intervertebral Disc / Q000166:cytology / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis* / Q000235:genetics
D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C505528:Casp8 protein, rat; D053181:Caspase 8; D020169:Caspases