Comparison of three Legionella urinary antigen assays during an outbreak of legionellosis in Belgium.
During an outbreak of legionellosis in Belgium, urine samples of 32 legionellosis patients were tested with three Legionella urinary antigen assays: the Biotest enzyme immunoassay (EIA) kit, the Binax EIA kit and the Binax NOW Immunochromatographic Test kit. The three tests were concomitantly compared. The test sensitivities on the first urine samples were 65.6 % for the Biotest EIA, 50.0 % for the Binax EIA and 56.3 % for the Binax NOW. Testing of a second urine sample increased the sensitivities to 71.9 %, 59.4 % and 65.6 %, respectively. The differences were not statistically significant. In outbreak settings, testing second samples from patients presenting with symptoms but initially testing negative and/or concentrating urine samples for testing might be valuable additions to the urinary antigen test to increase the sensitivities of the tests.
Dirven
Kristien
K
Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
Ieven
Margareta
M
Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
Peeters
Marcel F
MF
Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
van der Zee
Anneke
A
Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
De Schrijver
Koen
K
Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
Goossens
Herman
H
Laboratory of Medical Microbiology, University of Antwerp UA, Wilrijk, Belgium 2Laboratory of Clinical Microbiology, University Hospital Antwerp UZA, Wilrijkstraat 10, B-2650 Edegem, Antwerp, Belgium 3Laboratory of Molecular Microbiology, St Elisabeth Hospital, Hilvarenbeekseweg 60, NL-5000 AS Tilburg, The Netherlands 4Health Inspectorate of the Province of Antwerp, Ministry of the Flemish Community, Antwerp, Belgium.
D000942:Antigens, Bacterial / Q000652:urine*; D001530:Belgium / Q000453:epidemiology; D004196:Disease Outbreaks; D006801:Humans; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D007875:Legionella / Q000276:immunology* / Q000302:isolation & purification*; D007876:Legionellosis / Q000175:diagnosis / Q000453:epidemiology* / Q000652:urine; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D011933:Reagent Kits, Diagnostic