Generation of a conditional allele of the B-myb gene.
B-Myb is an essential transcription factor involved in control of the cell cycle and the regulation of tissue-specific gene expression in a wide range of cell types. Loss of both alleles results in early embryonic lethality at E4.5-6.5. To address the function of B-Myb in later stages of embryogenesis and in specific adult tissues, a floxed B-myb allele (B-mybF) was generated. Cre-mediated deletion in vivo was demonstrated by breeding with a transgenic GATA-Cre mouse line. An intermediate allele produced in the creation of the floxed allele, in which the PGK-neo(R) cassette is present in intron 3 (B-myb(loxneo)), was deduced to be a weak hypomorph based on the later embryonic death of homozygotes compared to B-myb(-/-) embryos. To demonstrate the efficiency and possible consequences of B-myb inactivation, we performed conditional deletion in cultured MEFs and observed decreased growth that correlated with aberrant nuclear DNA replication.
García
Paloma
P
Institute for Biomedical Research, Birmingham University Medical School, Edgbaston, United Kingdom.
Berlanga
Oscar
O
Watson
Roger
R
Frampton
Jon
J
D000483:Alleles; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D003433:Crosses, Genetic; D004261:DNA Replication; D005838:Genotype; D006720:Homozygote; D007438:Introns; D051379:Mice; D018345:Mice, Knockout; D011995:Recombination, Genetic; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D018797:Cell Cycle Proteins; D014157:Transcription Factors