Distributional characteristics of sulfated glycosaminoglycans in normal human nasal mucosa and nasal polyp.
The results of this study suggest that an altered expression pattern of sulfated glycosaminoglycans (GAGs) in nasal polyp may not function as a charge restriction barrier for vascular permeability, contributing to the leakage of protein and fluid. Sulfated GAGs are found on the vascular endothelial surface and in the extracellular matrix in various tissues and organs, suggesting that these materials constitute a negatively charged screen restricting the movement of circulating plasma molecules. This study was designed to elucidate the distributional characteristics of sulfated GAGs in normal human nasal mucosa and nasal polyp in order to understand their roles in the formation of nasal polyp. The expression and localization of sulfated GAGs in normal human nasal mucosa and nasal polyp were detected light microscopically with the histochemical method using poly-L-lysine-conjugated colloidal gold followed by silver enhancement. Sulfated GAGs in normal human nasal mucosa were distributed in the epithelial layer, vascular endothelial surface, submucosal gland and extracellular matrix. In nasal polyp, intense staining was also seen in the glandular structure and epithelial layer. However, the vascular endothelium and extracellular matrix exhibited either a weak reaction or no reaction.
Lee
Sang Hag
SH
Department of Otorhinolaryngology-Head & Neck Surgery, College of Medicine, Korea University, Seoul, South Korea. sanghag@kumc.or.kr
Oh
Joon Whan
JW
Lee
Heung Man
HM
Jung
Hak Hyun
HH
Lee
Seung Hoon
SH
Jang
Ji Won
JW
Jhun
Hyun Soo
HS
Jhun
Young Joon
YJ
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D006046:Gold; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism*; D009298:Nasal Polyps / Q000378:metabolism*; D011107:Polylysine; D013194:Staining and Labeling; D014420:Turbinates / Q000378:metabolism*
D006025:Glycosaminoglycans; C089532:cationic colloidal gold; D011107:Polylysine; D006046:Gold