[Fusion and apoptosis of epithelial cells induced by muramidase released protein of Streptococcus suis type 2].
The pathogenic role of muramidase released protein (MRP), a virulence factor of Streptococcus suis type 2 (SS2) is poorly understood. The purified MRP was co-incubated with HEp-2 cells to determine the effect of MRP on epithelial cells. Under light microscope, two principal morphologic changes were observed. Firstly, the cells were fused to form syncytia and a apoptosis followed. Secondly, single cell was also induced to apoptosis at high level as 18%, which was verified by transmission electron microscopy and flow cytometry. It showed that MRP alone was capable of a virulence factor of SS2.
Zeng
Qiaoying
Q
Key Laborary of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agric Univ, Nanjing 210095, China. zqyziyi@yahoo.com.cn
Lu
Chengping
C
D000942:Antigens, Bacterial / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001426:Bacterial Proteins / Q000494:pharmacology*; D002459:Cell Fusion; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D013290:Streptococcal Infections / Q000382:microbiology*; D017011:Streptococcus suis / Q000737:chemistry / Q000378:metabolism*; D037521:Virulence Factors / Q000494:pharmacology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C421489:MRP protein, Streptococcus suis; D037521:Virulence Factors