RFX-1, a putative alpha Adducin interacting protein in a human kidney library.
Adducin regulates tubular absorption of sodium by modulating the expression levels of the sodium-potassium-ATPase in renal tubular cells. Adducin is a candidate gene in the pathogenesis of hypertension. Yeast two hybrid screen showed a specific interaction between human alpha Adducin and the regulatory factor for X box (RFX-1), a nuclear protein that down regulates the expression of several proteins in non neuronal cells. The interaction was confirmed in cells through co-immunoprecipitation and colocalization experiments. The binding of alpha Adducin to RFX-I and their nuclear co-localization suggests that Adducin can have a role in modulating the transcriptional regulating activity of RFX-I.
Boito
Rosalia
R
Dipartimento di Medicina Sperimentale e Clinica "G. Salvatore", Università Magna Graecia, Catanzaro, Campus di Germaneto, Viale Europa, 88100 Catanzaro, Italy; Unit of Nephrology, Policlinico Universitario "Mater Domini", Catanzaro, Italy.
Menniti
Miranda
M
Amato
Rosario
R
Palmieri
Camillo
C
Marinaro
Cinzia
C
Iuliano
Rodolfo
R
Tripodi
Grazia
G
Cusi
Daniele
D
Fuiano
Giorgio
G
Perrotti
Nicola
N
D000818:Animals; D019556:COS Cells; D002148:Calmodulin-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015723:Gene Library; D006801:Humans; D047468:Immunoprecipitation; D007668:Kidney / Q000378:metabolism*; D000071701:Regulatory Factor X Transcription Factors; D000071716:Regulatory Factor X1; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D000099291:Adducins
D002148:Calmodulin-Binding Proteins; D004268:DNA-Binding Proteins; D000071701:Regulatory Factor X Transcription Factors; D000071716:Regulatory Factor X1; D014157:Transcription Factors; D000099291:Adducins; C000605537:RFX1 protein, human