Association of CD14 promoter polymorphisms and soluble CD14 levels in mite allergen sensitization of children in Taiwan.
CD14 is responsible for environmental lipopolysaccharide recognition and is a positional candidate gene for allergy. We hypothesized that genetic polymorphisms in the promoter region of the CD14 gene may be associated with Dermatophagoides pteronysinnus (Der p) allergen sensitization in children. Three single nucleotide polymorphisms (SNPs) of the CD14 promoter region, C(-159)T, A(-1,145)G, and G(-1,359)T were genotyped, and analyzed in 240 randomized case-control school-age children in Taiwan. Serum concentrations of IgE and soluble CD14 (sCD14) were also assayed. We found a significant inverse correlation of sCD14 and total serum IgE levels in our study population. Moreover, sCD14 binds Der p allergen in vitro in a dose-dependent manner. The distribution of three SNPs genotypes was similar in asthmatic children and the control group. However, there was a significant difference in the distribution of genotype CD14 G(-1,359)T, but not C(-159)T, between mite-sensitive and non-sensitive children. Haplotype analysis showed strong linkage disequilibrium among these three SNPs in the CD14 promoter region. Carriers of the CD14-159C/-1,145A/-1,359T haplotype had the highest IgE and lowest sCD14 levels as compared to other haplotypes. Our results support the hypothesis that CD14 gene variants may play an important role in influencing allergen sensitization of children in Taiwan.
Tan
Choon-Yee
CY
Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.|Institute of Basic Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Chen
Yi-Lin
YL
Department of Pathology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Wu
Lawrence Shih-Hsin
LS
Vita Genomics, Wugu, Taiwan.
Liu
Chai-Fan
CF
Institute of Basic Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Chang
Wen-Tsan
WT
Institute of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Wang
Jiu-Yao
JY
Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. a122@mail.ncku.edu.tw.|Institute of Basic Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. a122@mail.ncku.edu.tw.|Division of Allergy and Clinical Immunology, Department of Pediatrics, College of Medicine, National Cheng Kung University, 138 Sheng-Li road, Tainan, 70428, Taiwan. a122@mail.ncku.edu.tw.
D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D001483:Base Sequence; D002648:Child; D017931:DNA Primers; D008040:Genetic Linkage; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008925:Mites / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D013624:Taiwan
D039741:Antigens, Dermatophagoides; D017931:DNA Primers; D018950:Lipopolysaccharide Receptors