[Relationship between key enzyme activities of inosine-producing pathway and inosine accumulation].
The specific activities of key enzymes relating to the pathway of inosine synthesis of three different bacterial strains including high-yield, low-yield and wild strains were determined and compared systematically. A close relationship between inosine production and the specific activities of key enzymes was found. According to the enzyme characteristics of high-yield strain, suggestions on further strain improvement by modification of genetic engineering were proposed. Enzymology study is believed to be an effective way to make screening of high-yield strains more efficient.
Song
Yongbo
Y
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. songyongbo@sina.com
Cai
Xianpeng
X
Chu
Ju
J
Zhuang
Yingping
Y
Zhang
Siliang
S
D000265:Adenylosuccinate Synthase / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways; D007168:IMP Dehydrogenase / Q000235:genetics / Q000378:metabolism; D007288:Inosine / Q000378:metabolism*; D000637:Transaminases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D007288:Inosine; D007168:IMP Dehydrogenase; D000637:Transaminases; C022389:phosphoribosyl pyrophosphate aminotransferase; D000265:Adenylosuccinate Synthase