26-Locus Y-STR typing in a Bhutanese population sample.
26 Y chromosome short tandem repeat (STR) loci were amplified in a sample of 856 unrelated males from Bhutan, using two multiplex polymerase chain reaction (PCR) assays. The first multiplex is the Y-STR 20plex described by Butler et al. [J.M. Butler, R. Schoske, P.M. Vallone, M.C. Kline, A.J. Redd, M.F. Hammer, A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers, Forensic Sci. Int. 129 (2002) 10-24], and the second is a novel (but overlapping) 14plex that targets six additional Y-STRs (DYS425, DYS434, DYS435, DYS436, DYS461, DYS462) and also amplifies the amelogenin locus. The 26-loci give a discriminating power of 0.9957, though even at this resolution one haplotype occurs 24 times. We identify novel alleles at five loci and microvariants at a further three, which were characterised by sequencing. Extended (11-locus) haplotypes for these samples have been submitted to the Y-STR Haplotype Reference Database (YHRD).
Parkin
Emma J
EJ
Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK.
Kraayenbrink
Thirsa
T
van Driem
George L
GL
Tshering Of Gaselô
Karma
K
de Knijff
Peter
P
Jobling
Mark A
MA
D053523:Amelogenin; D001631:Bhutan; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting; D003746:Dental Enamel Proteins / Q000235:genetics; D005787:Gene Frequency; D005828:Genetics, Population; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
D053523:Amelogenin; D003746:Dental Enamel Proteins