Novel enzyme immunoassay utilizing lipopolysaccharide-binding protein as a capture molecule for the measurement of chlamydial lipopolysaccharide in serum.
Chlamydia pneumoniae causes respiratory tract infections. It has a tendency to cause persistent infections, which have been associated with several chronic diseases (e.g., atherosclerosis). At present, there is no reliable method for the diagnosis of chronic C. pneumoniae infection. We developed a novel enzyme immunoassay (EIA) for the quantification of chlamydial lipopolysaccharide (cLPS) in human serum. Serum cLPS was solubilized with detergent and then captured by LPS-binding protein (LBP). LBP-LPS complexes were bound to the solid phase with anti-cLPS monoclonal antibody, and the bound complexes were detected with anti-LBP antibodies. The new method was used to quantify serum cLPS in acute coronary syndrome (ACS) patients (n = 102) and their healthy controls. cLPS was detected in 77.5% of ACS patients and in 52% of controls (P < .001) with geometric mean concentrations of 1.87 and 0.61 microg/mL (P < .001), respectively. The novel cLPS EIA method will provide a potential diagnostic tool for C. pneumoniae infection.
Tiirola
Terttu
T
Department of Molecular Medicine, National Public Health Institute, Biomedicum, PO Box 104, FIN-00251 Helsinki, Finland. terttu.tiirola@ktl.fi
Jaakkola
Anne
A
Bloigu
Aini
A
Paldanius
Mika
M
Sinisalo
Juha
J
Nieminen
Markku S
MS
Silvennoinen-Kassinen
Sylvi
S
Saikku
Pekka
P
Jauhiainen
Matti
M
Leinonen
Maija
M
D000209:Acute-Phase Proteins / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D002352:Carrier Proteins / Q000276:immunology*; D016993:Chlamydophila pneumoniae / Q000737:chemistry*; D006801:Humans; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000097:blood*; D008562:Membrane Glycoproteins / Q000276:immunology*; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D000907:Antibodies, Bacterial; D002352:Carrier Proteins; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D000099231:Lipopolysaccharide-Binding Protein