Positional proteomics: selective recovery and analysis of N-terminal proteolytic peptides.
Bottom-up proteomics is the analysis of peptides derived from single proteins or protein mixtures, and because each protein generates tens of peptides, there is scope for controlled reduction in complexity. We report here a new strategy for selective isolation of the N-terminal peptides of a protein mixture, yielding positionally defined peptides. The method is tolerant of several fragmentation methods, and the databases that must be searched are substantially less complex.
McDonald
Lucy
L
Protein Function Group, Faculty of Veterinary Science, University of Liverpool, Crown Street, Liverpool L69 7ZJ, UK.
Robertson
Duncan H L
DH
Hurst
Jane L
JL
Beynon
Robert J
RJ
D000818:Animals; D019921:Biotinylation; D013058:Mass Spectrometry; D051379:Mice; D009124:Muscle Proteins / Q000737:chemistry / Q000302:isolation & purification; D018482:Muscle, Skeletal / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification; D010449:Peptide Mapping; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D009124:Muscle Proteins; D010446:Peptide Fragments; D011506:Proteins