title: Bacillus ruris sp. nov., from dairy farms.
abstract: Four novel ellipsoidal spore-forming Bacillus isolates with swollen sporangia, isolated from raw milk and feed concentrate, showed a high level of similarity in SDS-PAGE, fatty acid methyl esters and routine phenotypic tests. However, 16S rRNA gene sequence comparisons showed that this taxon was different from other related Bacillus species, and only a low level of DNA relatedness was found with the closest phylogenetic and phenotypic relative, Bacillus galactosidilyticus. This taxon could be differentiated from B. galactosidilyticus on the basis of morphological differences, stronger acid reactions with a wide range of substrates after 48 h incubation, and qualitative and quantitative differences in fatty acid content. On the basis of these data, a novel species, Bacillus ruris sp. nov., is proposed, with LMG 22866T (=DSM 17057T) as the type strain.
authors.affiliation: Department of Animal Product Quality, Centre for Agricultural Research-Ghent, Brusselsesteenweg 370, B-9090 Melle, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Heyndrickx
authors.affiliation: Department of Animal Product Quality, Centre for Agricultural Research-Ghent, Brusselsesteenweg 370, B-9090 Melle, Belgium.
authors.forename: Patsy
authors.initials: P
authors.lastname: Scheldeman
authors.affiliation: Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
authors.forename: Gillian
authors.initials: G
authors.lastname: Forsyth
authors.affiliation: Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Liesbeth
authors.initials: L
authors.lastname: Lebbe
authors.affiliation: Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
authors.forename: Marina
authors.initials: M
authors.lastname: Rodríguez-Díaz
authors.affiliation: Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
authors.forename: Niall A
authors.initials: NA
authors.lastname: Logan
authors.affiliation: Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: De Vos
mesh_terms: D000821:Animal Feed / Q000382:microbiology*; D000818:Animals; D001407:Bacillus / Q000145:classification / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D003612:Dairying; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial
chemical_list: D012336:RNA, Ribosomal, 16S