title: Beta-catenin regulates myogenesis by relieving I-mfa-mediated suppression of myogenic regulatory factors in P19 cells.
abstract: Wnt/beta-catenin signaling plays a critical role in embryonic myogenesis. Here we show that, in P19 embryonic carcinoma stem cells, Wnt/beta-catenin signaling initiates the myogenic process depends on beta-catenin-mediated relief of I-mfa (inhibitor of MyoD Family a) suppression of myogenic regulatory factors (MRFs). We found that beta-catenin interacted with I-mfa and that the interaction was enhanced by Wnt3a. In addition, we found that the interaction between beta-catenin and I-mfa was able to attenuate the interaction of I-mfa with MRFs, relieve I-mfa-mediated suppression of the transcriptional activity and cytosolic sequestration of MRFs, and initiate myogenesis in a P19 myogenic model system that expresses exogenous myogenin. This work reveals a mechanism for the regulation of MRFs during myogenesis by elucidating a beta-catenin-mediated, but lymphoid enhancing factor-1/T cell factor independent, mechanism in regulation of myogenic fate specification and differentiation of P19 mouse stem cells.
authors.affiliation: Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Pan
authors.forename: Yingying
authors.initials: Y
authors.lastname: Jia
authors.forename: Jiyong
authors.initials: J
authors.lastname: Wang
authors.forename: Donglei
authors.initials: D
authors.lastname: Tao
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Gan
authors.forename: Leonidas
authors.initials: L
authors.lastname: Tsiokas
authors.forename: Naihe
authors.initials: N
authors.lastname: Jing
authors.forename: Dianqing
authors.initials: D
authors.lastname: Wu
authors.forename: Lin
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002458:Cell Fractionation; D045744:Cell Line, Tumor; D047468:Immunoprecipitation; D051379:Mice; D008856:Microscopy, Fluorescence; D024510:Muscle Development / Q000502:physiology*; D018006:Myogenic Regulatory Factors / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*; D013234:Stem Cells / Q000378:metabolism* / Q000502:physiology; D020798:Two-Hybrid System Techniques; D051176:beta Catenin / Q000378:metabolism*
chemical_list: C491077:Mdfi protein, mouse; D018006:Myogenic Regulatory Factors; D034741:RNA, Small Interfering; D051176:beta Catenin