Group IV cytosolic phospholipase A2 mediates arachidonic acid release in H9c2 rat cardiomyocyte cells in response to hydrogen peroxide.
Damaging reactive oxygen species are released during episodes of ischemia and reperfusion. Some cellular adaptive responses are triggered to protect the injured organ, while other cascades are triggered which potentiate the damage. In these studies, we demonstrate that rat cardiomyocte H9c2 cells release arachidonic acid in response to hydrogen peroxide. In H9c2 cells, arachidonic acid release is attenuated by methyl arachidonyl fluorophosphonate (MAFP) and pyrrophenone, indicating that a phospholipase A2 Group IV enzyme mediates arachidonic acid mobilization. Moreover, hydrogen peroxide alters the cellular morphology of the H9c2 cells, causing drastic cell shrinkage. Because MAFP and pyrrophenone prevent the morphological alterations caused by hydrogen peroxide, these studies show that phospholipase A2 Group IV activity is likely integrally involved in the damage initiated by hydrogen peroxide.
Winstead
Michelle V
MV
Department of Chemistry and Biochemistry and Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0601, USA.
Lucas
Karin Killermann
KK
Dennis
Edward A
EA
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D001095:Arachidonic Acids / Q000494:pharmacology; D001483:Base Sequence; D002460:Cell Line; D003600:Cytosol / Q000201:enzymology*; D017931:DNA Primers; D006321:Heart / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D009206:Myocardium / Q000201:enzymology*; D063065:Organophosphonates / Q000494:pharmacology; D010741:Phospholipases A / Q000235:genetics / Q000378:metabolism*; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
D001095:Arachidonic Acids; D017931:DNA Primers; D063065:Organophosphonates; D012333:RNA, Messenger; C104810:methyl arachidonylfluorophosphonate; D016718:Arachidonic Acid; D006861:Hydrogen Peroxide; D010741:Phospholipases A; D054467:Phospholipases A2