title: Synthesis of enantiopure (5R)-hydroxyhexane-2-one with immobilised whole cells of Lactobacillus kefiri.
abstract: Whole-cell reduction of (2,5)-hexanedione to yield highly enantiopure (5R)-hydroxyhexane-2-one (enantiomeric excess >99%) with Lactobacillus kefiri DSM 20587 was investigated. Cell immobilisation with sodium cellulose sulphate was chosen as the most suitable encapsulation matrix, giving an immobilisation yield of 40%. Despite the lowered biocatalytic activity from cell immobilisation, the bioreduction process was vastly improved with the help of reaction engineering techniques (batch to a plug flow reactor set-up). High selectivity (95%) and space-time yield (87 g L(-1) day(-1)) were achieved in the plug flow reactor. The biocatalyst remained active (68% residual activity) after 6 days of operation.
authors.affiliation: Institute of Biotechnology, Forschungszentrum Juelich GmbH, Germany.
authors.forename: A W I
authors.initials: AW
authors.lastname: Tan
authors.forename: M
authors.initials: M
authors.lastname: Fischbach
authors.forename: H
authors.initials: H
authors.lastname: Huebner
authors.forename: R
authors.initials: R
authors.lastname: Buchholz
authors.forename: W
authors.initials: W
authors.lastname: Hummel
authors.forename: T
authors.initials: T
authors.lastname: Daussmann
authors.forename: C
authors.initials: C
authors.lastname: Wandrey
authors.forename: A
authors.initials: A
authors.lastname: Liese
mesh_terms: D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D018914:Cells, Immobilized; D002482:Cellulose / Q000031:analogs & derivatives; D000441:Hexanols / Q000378:metabolism*; D007778:Lactobacillus / Q000166:cytology / Q000378:metabolism*
chemical_list: D000441:Hexanols; D002482:Cellulose