title: Functional analysis of a putative Ca2+ channel gene TaTPC1 from wheat.
abstract: The cytosolic free-calcium concentration [Ca2+](cyt) transiently increases under abiotic stresses and the proteins that control this process are gradually disclosed. The Ca2+-permeable channel is one type of these proteins in plants. In the present study, a novel Ca2+-permeable channel gene TaTPC1 encoding a putative membrane protein was cloned from wheat. It was induced under high salinity, polyethylene glycol, low temperature (4 degrees C), and abscisic acid. Expression of TaTPC1 in the yeast mutant lacking CCH1 can recover its growth under lithium stress through functional complementation. TaTPC1 transgenic plants exhibited more stomatal closing in the presence of Ca2+ than the control, supporting a role for the calcium channel in regulating plant responses to environmental change.
authors.affiliation: National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Jia-Ning
authors.initials: JN
authors.lastname: Yu
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zhang
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Hao
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Zhang
authors.forename: Shou-Yi
authors.initials: SY
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D003001:Cloning, Molecular; D015870:Gene Expression; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D030821:Plants, Genetically Modified / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
chemical_list: D015220:Calcium Channels; D010940:Plant Proteins; D002118:Calcium