Targeted gene delivery to cancer cells with nanometric DNA particles enveloped with folic acid using a polymerisable anchor.
Progress in the design of gene delivery system is vital for cancer gene therapy since many physiological and intracellular barriers remain. We have developed a technology for condensing genes into nanometric delivery systems. In this paper, we present a novel strategy for decorating 30 nm DNA particles with folic acid for cancer cell recognition. Physicochemical and biological experiments show that these DNA complexes selectively bind to cells expressing the corresponding folic acid receptor.
Zuber
Guy
G
Laboratoire de Chimie Genetique, Université Louis Pasteur, Faculté de Pharmacie, Illkirch, France. zuber@bioorga.u-strasbg.fr
Muller
Christian D
CD
Behr
Jean-Paul
JP
D002352:Carrier Proteins / Q000378:metabolism; D004247:DNA / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy; D006801:Humans; D007624:KB Cells; D008156:Luciferases / Q000235:genetics; D015394:Molecular Structure; D036103:Nanotechnology; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism
D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D011956:Receptors, Cell Surface; D011092:Polyethylene Glycols; D004247:DNA; D005492:Folic Acid; D008156:Luciferases