Enzymatic synthesis of L-ascorbyl linoleate in organic media.
A novel L-ascorbyl fatty acid ester, L-ascorbyl linoleate was successfully prepared by enzymatic esterification and transesterification in a non-aqueous medium using immobilized lipase as biocatalyst. Changes in enzymatic activity and product yield were studied for the following variable: the nature of the fatty acid, the fatty acid concentration and water content. The yield of synthesis for the C18 unsaturated fatty acids were higher than for the C18 saturated fatty acid. Initial enzyme concentration does not affect the equilibrium of the reaction. And the product yield (33.5%) in the transesterification was higher than that of the esterification (21.8%) at a high-substrate concentration 0.3 M. The medium water content was found to have a distinct influence on the L-ascorbyl linoleate synthesis.
Song
Qingxun
Q
State Key Laboratory of Bioreactor Engineering, New Word Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, PR China. qxsong@ecust.edu.cn
Zhao
Ying
Y
Xu
Weiqin
W
Zhou
Wenyu
W
Wei
Dongzhi
D
D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005656:Fungal Proteins; D019787:Linoleic Acid / Q000737:chemistry*; D008041:Linoleic Acids / Q000138:chemical synthesis* / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D008041:Linoleic Acids; D009930:Organic Chemicals; D012997:Solvents; C005575:methyl linoleate; D019787:Linoleic Acid; D008049:Lipase; C431349:lipase B, Candida antarctica; D001205:Ascorbic Acid; C502798:ascorbyl linoleate