Ubiquitination of APOBEC3 proteins by the Vif-Cullin5-ElonginB-ElonginC complex.
APOBEC3 proteins are antiviral host factors for a wide variety of retroviruses. HIV-1 Vif overcomes the antiviral activity of APOBEC3G by ubiquitinating the protein. In this study, we examined the ability of Vif to antagonize other family members of APOBEC3 proteins, together with its mechanism. Using HIV infectivity, virion incorporation, immunoprecipitation, and in vitro ubiquitin conjugation assays, we show that the ability of Vif to inhibit antiviral activity of APOBEC3 proteins positively correlates with its ability to bind and ubiquitinate these proteins by a Vif-Cullin5-ElonginB-ElonginC (Vif-BC-Cul5) complex. These results suggest that Vif exhibits its anti-APOBEC3 activity by the ubiquitin ligase activity of the Vif-BC-Cul5 complex.
Shirakawa
Kotaro
K
Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan.
Takaori-Kondo
Akifumi
A
Kobayashi
Masayuki
M
Tomonaga
Mitsunori
M
Izumi
Taisuke
T
Fukunaga
Keiko
K
Sasada
Amane
A
Abudu
Aierken
A
Miyauchi
Yasuhiro
Y
Akari
Hirofumi
H
Iwai
Kazuhiro
K
Uchiyama
Takashi
T
D000071480:APOBEC-3G Deaminase; D002460:Cell Line; D044842:Cullin Proteins / Q000737:chemistry / Q000378:metabolism*; D003564:Cytidine Deaminase / Q000737:chemistry / Q000378:metabolism; D043525:Cytosine Deaminase / Q000737:chemistry / Q000378:metabolism; D000075065:Elongin; D015967:Gene Expression Regulation, Viral; D016346:Gene Products, vif / Q000737:chemistry / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism; D006801:Humans; D015778:Minor Histocompatibility Antigens; D009700:Nucleoside Deaminases / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism*; D054320:vif Gene Products, Human Immunodeficiency Virus
D044842:Cullin Proteins; D000075065:Elongin; D016346:Gene Products, vif; D015778:Minor Histocompatibility Antigens; D012097:Repressor Proteins; D014157:Transcription Factors; D025801:Ubiquitin; D054320:vif Gene Products, Human Immunodeficiency Virus; D009700:Nucleoside Deaminases; C492906:APOBEC3F protein, human; D043525:Cytosine Deaminase; D000071480:APOBEC-3G Deaminase; C495794:APOBEC3B protein, human; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase