Functionalized porous silicon surfaces as MALDI-MS substrates for protein identification studies.
Alkyl monolayer modified porous silicon functional surfaces are employed for selective binding of proteins from complex mixtures (through washing of the deposited mixture spot using appropriate buffer) and MALDI-MS is used to detect the components retained on the surface.
Mengistu
Tadesse Z
TZ
Department of Chemistry, 4700 Keele Street, Toronto, Ontario M3J1P3, Canada.
DeSouza
Leroi
L
Morin
Sylvie
S
D016062:Porosity; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D012825:Silicon / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity
D011506:Proteins; D012825:Silicon