Cloning of a D-serine-regulated transcript dsr-2 from rat cerebral neocortex.
D-serine is now considered to be an endogenous co-agonist of the NMDA receptor in mammalian brain. To obtain insight into the molecular mechanisms underlying D-serine metabolism and function, we explored transcripts that are responsive to D-serine in the neocortex of the 8-day-old infant rat by a differential cloning technique, RNA arbitrarily primed PCR. We isolated a novel D-serine inducible transcript, D-serine-responsive transcript-2 (dsr-2), that was exclusively expressed in the brain. Sequence analysis of the corresponding cDNAs to the transcript revealed that the dsr-2 mRNA consists of 7199 nucleotides with an open reading frame encoding 111 amino acids. The dsr-2 gene was located on the reverse strand within an intron of the neurexin-3alpha gene, mapped to rat chromosome 6q24-31. The regional distribution of the basal expression of dsr-2 and its ontogenic changes in the brain closely correlated with those of free D-serine and of NMDA receptor R2B subunit mRNA, but were somewhat different from those of the neurexin-3alpha transcript. These findings suggest that dsr-2 may be involved in D-serine metabolism and/or function, and in the interactions between D-serine, NMDA receptor and neurexin-3alpha, in mammalian brain.
Taniguchi
Go
G
Section of Psychiatry and Behavioral Sciences, Tokyo Medical and Dental University Graduate School, Tokyo, Japan.
Yamamoto
Naoki
N
Tsuchida
Hideto
H
Umino
Asami
A
Shimazu
Dai
D
Sakurai
Shin-ichiro
S
Takebayashi
Hironao
H
Nishikawa
Toru
T
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002490:Central Nervous System / Q000378:metabolism; D003001:Cloning, Molecular; D016172:DNA Fingerprinting; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D008969:Molecular Sequence Data; D019579:Neocortex / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016548:Prosencephalon / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012694:Serine / Q000502:physiology*; D014018:Tissue Distribution
D009419:Nerve Tissue Proteins; C507069:dsr-2 protein, rat; D012694:Serine; D012313:RNA; D000251:Adenosine Triphosphatases