title: Improved HPLC method for the simultaneous determination of allantoin, uric acid and creatinine in cattle urine.
abstract: An HPLC procedure developed for the rapid and simultaneous determination of purine derivatives (PD) in ruminants' urine was investigated, since the adoption of a single method for the simultaneous detection of PD and creatinine was not carried out due to elution of polar co-extractives and also due to overlapping of the peaks of allantoin and creatinine. The experimental conditions chosen in the present study avoid the presence of chemically competitive compounds and afford a good separation of the peaks of allantoin and creatinine. The recoveries of the standard compounds added to urine samples were 94-104%. This method can be proposed as a possible reference method for the estimation of allantoin, uric acid and creatinine in cattle urine.
authors.affiliation: Animal Nutrition Division, Indian Veterinary Research Institute, Izatnagar 243 122, UP, India. george.sherin@rediffmail.com
authors.forename: S K
authors.initials: SK
authors.lastname: George
authors.forename: M T
authors.initials: MT
authors.lastname: Dipu
authors.forename: U R
authors.initials: UR
authors.lastname: Mehra
authors.forename: P
authors.initials: P
authors.lastname: Singh
authors.forename: A K
authors.initials: AK
authors.lastname: Verma
authors.forename: J S
authors.initials: JS
authors.lastname: Ramgaokar
mesh_terms: D000481:Allantoin / Q000652:urine*; D000818:Animals; D002138:Calibration; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003404:Creatinine / Q000652:urine*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D014527:Uric Acid / Q000652:urine*
chemical_list: D014527:Uric Acid; D000481:Allantoin; D003404:Creatinine