Evaluation of different plasmid DNA delivery systems for immunization against HER2/neu in a transgenic murine model of mammary carcinoma.
Studies of DNA vaccination against HER2/neu showed the effectiveness of immunization protocols in models of transplantable or spontaneous tumors; scarce information, however, has been provided to identify the procedure of DNA administration that more effectively contributes to the activation of immune system against spontaneously arising HER2/neu-positive tumors. We compared the effectiveness of different procedures of DNA vaccine delivery (intradermic injection (ID), gene gun (GG) delivery and intramuscular injection (IM) alone or with electroporation) in a murine transgenic model of mammary carcinoma overexpressing HER2/neu. We highlighted the role of DNA delivery system in the success of DNA vaccination showing that, among the analysed methods, intramuscular injection of the vaccine, particularly when associated to electroporation, elicits a better protection against HER2/neu spontaneous tumor development inducing antibody and cell-mediated immune responsiveness against HER2/neu and a Th1 polarization of the immune response.
Smorlesi
Arianna
A
Laboratory of Tumor Immunology, Immunology Center, INRCA Gerontology Research Department, Via Birarelli 8, 60121 Ancona, Italy.
Papalini
Francesca
F
Amici
Augusto
A
Orlando
Fiorenza
F
Pierpaoli
Sara
S
Mancini
Chiara
C
Provinciali
Mauro
M
D000818:Animals; D000912:Antibodies, Neoplasm / Q000097:blood; D019470:Biolistics; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology; D016207:Cytokines / Q000032:analysis; D003601:Cytotoxicity Tests, Immunologic; D004195:Disease Models, Animal; D018274:Electroporation; D005260:Female; D018734:Genes, erbB-2; D007116:Immunization, Passive; D007271:Injections, Intradermal; D007273:Injections, Intramuscular; D008325:Mammary Neoplasms, Experimental / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008822:Mice, Transgenic; D010957:Plasmids; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*
D000912:Antibodies, Neoplasm; D019496:Cancer Vaccines; D016207:Cytokines; D019444:Vaccines, DNA