[Bone defect repair with a new tissue-engineered bone carrying bone morphogenetic protein in rabbits].
OBJECTIVE
To construct a new tissue-engineered bone with poly (D, L-lactide-co-glycolide) (PLGA), bone morphogenetic protein (BMP) and bone marrow-derived stem cells (BMSCs) and observe its effect in repairing segmental bone defects.


METHODS
A 15-mm bone defect in the right radius was induced in New Zealand white rabbits, and the models were randomized into three groups to receive implantation of the tissue-engineered bone grafts constructed with PLGA carrying 5 mg BMP and about 1 x 10(6) BMSCs (experimental group), grafts of PLGA with about 1 x 10(6) BMSCs (control group), or grafts of exclusive PLGA (blank control group), respectively. The osteogenesis in the bone defect after the implantation on was evaluated X-ray films, and the histological changes of the tissues sampled from the bone defect 4, 8, and 12 weeks after operation were observed and new bone formation was measured by image analysis.


RESULTS
The bone defect was completely repaired in the experimental group 12 weeks after the implantation, showing the best results among the 3 groups. The bone defects in the blank control group was filled with only fibrous and connective tissues at 12 weeks.


CONCLUSIONS
This tissue-engineered bone constructed with PLGA, BMP and BMSCs possesses good ability in repairing segmental bone defect.
Hu
Ji-jie
JJ
Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. hjj0610@163.com
Jin
Dan
D
Quan
Da-ping
DP
Zhong
Shi-zhen
SZ
Chen
Jiong-hao
JH
Wei
Kuan-Hai
KH
Zhao
Jie
J
Pei
Guo-xian
GX
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes; D002478:Cells, Cultured; D005260:Female; D020007:Implants, Experimental; D019344:Lactic Acid / Q000627:therapeutic use*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011100:Polyglycolic Acid / Q000627:therapeutic use*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000627:therapeutic use*; D011817:Rabbits; D011885:Radius Fractures / Q000601:surgery*; D011897:Random Allocation; D023822:Tissue Engineering
D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid