The leukocyte protein L-plastin induces proliferation, invasion and loss of E-cadherin expression in colon cancer cells.
L-plastin, a gene that codes for an actin-bundling protein, is upregulated in the metastatic colon cancer cell line SW620, when compared to its premetastatic counterpart SW480. The aim of our study was to characterise the effect of L-plastin overexpression on SW480 cells in the context of the acquisition of a metastatic phenotype. SW480 cell lines overexpressing L-plastin were established (SW480-LPL). Analysis of these cell lines revealed significantly higher rates of proliferation and invasion than the control cell line (SW480-Ctrl). In addition, the expression of E-cadherin was lost from SW480-LPL cells. Treatment of SW480-LPL cells with cytochalasin B, an inhibitor of endocytosis, attenuated the loss of E-cadherin expression in these cells. The association of L-plastin overexpression with an increased rate of proliferation and invasion, and loss of E-cadherin expression in the SW480 colon cancer cell line indicates that L-plastin plays an important mechanistic role in colorectal cancer metastasis (supplementary material for this article can be found on the International Journal of Cancer website at http://www.interscience.wiley.com/jpages/0020-7136/suppmat/index.html).
Foran
Eilis
E
Department of Biochemistry, Royal College of Surgeons in Ireland, Dublin 2, and Department of Clinical Medicine, Trinity College Dublin, St. James's Hospital, Dublin 8, Republic of Ireland.
McWilliam
Peter
P
Kelleher
Dermot
D
Croke
David T
DT
Long
Aideen
A
D001798:Blood Proteins / Q000096:biosynthesis* / Q000502:physiology; D015820:Cadherins / Q000096:biosynthesis*; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D020869:Gene Expression Profiling; D006801:Humans; D008840:Microfilament Proteins; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D001798:Blood Proteins; D015820:Cadherins; C047262:LCP1 protein, human; D008840:Microfilament Proteins