title: Activated cyclic AMP-response element binding protein (CREB) is expressed in a myelin-associated protein in chick.
abstract: Cyclic AMP response element (CRE) is a specific DNA sequence, which mediates transcriptional activation in the response to the cyclic AMP-activated and protein kinase A dependent signaling pathway. In the present study, phosphorylated CRE binding protein (CREB) immunoreactivity was mainly localized in the white matter of chick central nervous system (CNS). We have further confirmed the specificity of phospho-CREB immunoreaction in myelin using demyelinated optic nerve induced by lysophophatidylcholine (LPC), which is known to produce demyelination with little axonal damage. Double immunofluorescent analyses with myelin basic protein (MBP) and transferrin binding protein (TfBP), oligodendrocyte marker showed that phospho-CREB recognized a myelin-related protein in chick. Immunoblot analyses showed that phospho-CREB recognized a protein with molecular weights of approximately 70 kDa. Our data suggest that the antigen recognized by phospho-CREB is a myelin-associated protein in the chick CNS.
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, Seoul, South Korea.
authors.forename: Dong Woon
authors.initials: DW
authors.lastname: Kim
authors.forename: Jae Hyuk
authors.initials: JH
authors.lastname: Chang
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Park
authors.forename: Gye Sun
authors.initials: GS
authors.lastname: Jeon
authors.forename: Je Hoon
authors.initials: JH
authors.lastname: Seo
authors.forename: Sa Sun
authors.initials: SS
authors.lastname: Cho
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D002645:Chickens; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D003711:Demyelinating Diseases; D006801:Humans; D007150:Immunohistochemistry; D008244:Lysophosphatidylcholines / Q000378:metabolism; D004676:Myelin Basic Protein / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism*; D009900:Optic Nerve / Q000166:cytology / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D033863:Transferrin-Binding Proteins / Q000378:metabolism
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D008244:Lysophosphatidylcholines; D004676:Myelin Basic Protein; D033863:Transferrin-Binding Proteins