Transcriptional up-regulation of histamine receptor-1 in epithelial, mucus and inflammatory cells in perennial allergic rhinitis.
BACKGROUND
Histamine receptors play an important role in the pathogenesis of nasal allergy. Activation of histamine receptor 1 (H1R) and 2 (H2R) can cause allergic symptoms which can be blocked effectively by antihistamines. H1R and H2R transcript levels have been found to be up-regulated in perennial - but not in seasonal - allergic rhinitis (AR). The present study aimed to explore H1R and H2R expression in complex tissues of the nasal mucosa of perennial allergic rhinitis (PAR).


METHODS
Ten patients with PAR and 13 non-AR subjects were recruited for the study by medical history, physical examination and laboratory screening tests. In this study, we have analysed single cells dissected from the nasal mucosa biopsies by laser-assisted microdissection. H1R mRNA expression was analysed in different cell types such as epithelial, endothelial, mucus and inflammatory cells isolated from the nasal mucosa of PAR in comparison with non-AR subjects.


RESULTS
H1R mRNA gene expression level was significantly increased in the nasal mucosa of PAR in comparison with non-AR (P<0.0001). H1R mRNA was significantly elevated in epithelial (P<0.001) and mucus cells (P<0.05) of PAR in comparison with non-AR whereas H1R gene expression levels in endothelial cells between both groups were not changed (P=0.23). Interestingly, inflammatory cells in the nasal mucosa of PAR patients were also strongly expressed H1R mRNA (P<0.001).


CONCLUSIONS
The present study indicates that PAR alters the expression of H1R mRNA in epithelial, mucus and inflammatory cells of the nasal mucosa and but not in endothelial cells. Therefore, epithelial, mucus and inflammatory cells may play an important role in histamine-mediated allergic airway inflammation in PAR.
Dinh
Q T
QT
Department of Internal Medicine, Charité- Universitätsmedizin Berlin, Berlin, Germany. q-thai.dinh@charite.de
Cryer
A
A
Dinh
S
S
Peiser
C
C
Wu
S
S
Springer
J
J
Hamelmann
E
E
Klapp
B F
BF
Heppt
W
W
Fischer
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D042783:Endothelial Cells / Q000737:chemistry; D004847:Epithelial Cells / Q000737:chemistry; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009093:Mucus / Q000737:chemistry; D009297:Nasal Mucosa / Q000737:chemistry / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D011969:Receptors, Histamine H1 / Q000235:genetics*; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics* / Q000473:pathology; D014158:Transcription, Genetic / Q000235:genetics
D012333:RNA, Messenger; D011969:Receptors, Histamine H1