title: Characterization of vibrio parahaemolyticus isolated from coastal seawater in peninsular Malaysia.
abstract: Twenty-one Vibrio parahaemolyticus isolates representing 21 samples of coastal seawater from three beaches in peninsular Malaysia were found to be sensitive to streptomycin, norfloxacin and chloramphenicol. Resistance was observed to penicillin (100%), ampicillin (95.2%), carbenicilin (95.2%), erythromycin (95.2%), bacitracin (71.4%), cephalothin (28.6%), moxalactam (28.6%), kanamycin (19.1%), tetracycline (14.3%), nalidixic acid (9.5%) and gentamicin (9.5%). Plasmids of 2.6 to 35.8 mDa were detected among plasmid-containing isolates. All isolates carried the Vp-toxR gene specific to V. parahaemolyticus and were negative for the tdh gene, but only one isolate was positive for the trh gene. DNA fingerprinting of the isolates using ERIC-PCR and PFGE showed that the isolates belong to two major clonal groups, with several isolates from different locations in the same group, indicating the presence of similar strains in the different locations.
authors.affiliation: Department of Food Science, Faculty of Food Science and Technology, University Putra Malaysia, Serdang, Selangor.
authors.forename: Gwendelynne Bulan
authors.initials: GB
authors.lastname: Tanil
authors.forename: Son
authors.initials: S
authors.lastname: Radu
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Nishibuchi
authors.forename: Raha Abdul
authors.initials: RA
authors.lastname: Rahim
authors.forename: Suhaimi
authors.initials: S
authors.lastname: Napis
authors.forename: Lesley
authors.initials: L
authors.lastname: Maurice
authors.forename: Jurin Wolmon
authors.initials: JW
authors.lastname: Gunsalam
mesh_terms: D000818:Animals; D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008296:Malaysia; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D014735:Vibrio Infections / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000145:classification* / Q000302:isolation & purification / Q000472:pathogenicity*; D014871:Water Microbiology
chemical_list: D017931:DNA Primers