Intra-species variation of genotypes of Exophiala jeanselmei isolated from patients in Japan.
Isolates of Exophiala jeanselmei have been classified into 15 types based on their mitochondrial DNA (mtDNA). Thirteen of the 15 types and E. spinifera, which has been classified as E. jeanselmei Type 14, were confirmed to be also clearly differentiated by restriction fragment length polymorphism (RFLP) in internal transcribed spacer (ITS) regions of ribosomal RNA genes in their nuclear DNA (nDNA). Twenty strains of E. jeanselmei, newly identified or isolated from patients in Japan, were examined for mtDNA-RFLP and ITS-RFLP. The twenty isolates were comprised of: 11 E. jeanselmei Type 5, 6 E. jeanselmei Type 6, 2 Type 10, and 1 Type 8. E. jeanselmei Type 6 was the second most common strain in Japan after Type 5. Type 5 was definitely identified as E. jeanselmei var. jeanselmei and Type 8 was identified as E. jeanselmei var. lecanii-corni based on the genotypes of type strains of these species. However, two other types were still designated as E. jeanselmei Type 6 and E. jeanselmei Type 10.
Kawasaki
Masako
M
Department of Dermatology, Kanazawa Medical University, Ishikawa, Japan.
Anzawa
Kazushi
K
Tanabe
Hiroshi
H
Mochizuki
Takashi
T
Ishizaki
Hiroshi
H
Nishimura
Kazuko
K
D004272:DNA, Mitochondrial / Q000235:genetics; D005093:Exophiala / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005800:Genes, Fungal / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D009181:Mycoses / Q000382:microbiology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length
D004272:DNA, Mitochondrial