Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis.
Adoptively transferred dendritic cells presenting antigens derived from different pathogens have been shown to elicit specific T cell responses and to induce protective antibacterial immunity. We describe here the induction of high levels of protective immunity in mice using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis. We provide evidence that protection is superior to BCG and that it is associated with increased priming of CD4+ and CD8+ T cells specific for mycobacterial antigens. This method for generating high levels of anti-bacterial protective immunity could be helpful in the design of novel vaccines against tuberculosis and other intracellular pathogens.
Roy
Eleanor
E
Mycobacterial Division, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
De Silva
A Dharshan
AD
Sambandamurthy
Vasan K
VK
Clark
Simon O
SO
Stavropoulos
Evangelos
E
Jacobs
William R
WR
Brennan
John
J
Chan
John
J
Williams
Ann
A
Colston
M Joseph
MJ
Tascon
Ricardo E
RE
D019264:Adoptive Transfer; D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology / Q000637:transplantation; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D032581:Tuberculosis Vaccines / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology / Q000517:prevention & control*
D032581:Tuberculosis Vaccines