title: Apical cell adhesion molecule, trophinin, localizes to the nuclear envelope.
abstract: Trophinin mediates homophilic and apical cell adhesion between trophoblastic cells and endometrial epithelial cells, which is potentially the initial attachment step in human embryo implantation. Since trophinin is an atypical membrane protein without the signal sequence, it is possible that trophinin localizes to the cytoplasm. By treating trophinin-expressing trophoblastic cells with a series of detergents, we found significant levels of endogenous trophinin in the cytoplasm, particularly at the nuclear envelope (NE). Fluorescence photobleaching of GFP-trophinin expressed in COS-1 cells showed the stable association of trophinin with the NE, suggesting an additional role of trophinin besides apical cell adhesion.
authors.affiliation: Department of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Aoyama
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakayama
authors.forename: Daijiro
authors.initials: D
authors.lastname: Sugiyama
authors.forename: Sakura
authors.initials: S
authors.lastname: Saburi
authors.forename: Daita
authors.initials: D
authors.lastname: Nadano
authors.forename: Michiko N
authors.initials: MN
authors.lastname: Fukuda
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D019556:COS Cells; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000235:genetics; D002522:Chlorocebus aethiops; D003902:Detergents / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D009685:Nuclear Envelope / Q000737:chemistry*; D038761:Photobleaching; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects
chemical_list: D015815:Cell Adhesion Molecules; D003902:Detergents; C093649:TRO protein, human; D049452:Green Fluorescent Proteins