title: Hydrolyses and transglycosylations performed by purified alpha-D-glucosidase of the marine mollusc Aplysia fasciata.
abstract: The purification and characterisation of the alpha-glucosidase from the marine mollusc Aplysia fasciata are reported. Overall substrate specificity of the pure enzyme for both hydrolytic and transglycosylation reactions was studied. Remarkable characteristics of this enzyme are indicated by the results of the interesting survey of transglycosylation reactions reported: pyridoxine glucosylation, synthesis of chromophoric (pNP) di- and trisaccharides, glucosylation of cellobiose and sucrose. For these last two acceptors both the yields of reactions and the concentrations of products are comparable to those obtained using glycosyl transferases; in addition, synthesis of pyridoxine and chromophoric glycosides were still possible using a 1:1 ratio maltose:acceptor which is a very interesting characteristic from a synthetic point of view (effortless purification, productivity of each reaction batch, etc.).
authors.affiliation: Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, Via Campi Flegrei 34, 80078 Pozzuoli, Naples, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Andreotti
authors.forename: Assunta
authors.initials: A
authors.lastname: Giordano
authors.forename: Annabella
authors.initials: A
authors.lastname: Tramice
authors.forename: Ernesto
authors.initials: E
authors.lastname: Mollo
authors.forename: Antonio
authors.initials: A
authors.lastname: Trincone
mesh_terms: D000818:Animals; D001048:Aplysia / Q000201:enzymology*; D002384:Catalysis; D002475:Cellobiose / Q000096:biosynthesis; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008320:Maltose / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature; D014312:Trisaccharides / Q000096:biosynthesis; D000520:alpha-Glucosidases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D014312:Trisaccharides; C026440:glucosylsucrose; D002475:Cellobiose; D008320:Maltose; D000520:alpha-Glucosidases