Display and selection of chicken IgA Fab fragments.
Passive immune therapy is regaining interest to prevent and cure infectious diseases both in human and veterinary medicine. Therefore, systems are required that enable efficient targeted selection of antibodies originating from virtually any animal species. Here, a system for the selection of chicken IgA, using phage display, is described. A novel phagemid vector (pChick3) for the display and selection of chicken IgA antibodies in Fab format was developed. The functionality of pChick3 was demonstrated by construction of an immune antibody library using B cells from chickens infected with Eimeria acervulina. From this library, 10 different IgA fragments with specific binding to the E. acervulina antigen mix, the sporozoite or oocyst fractions were selected. These results demonstrate the efficiency and versatility of the pChick3 vector system that can readily be applied to construct libraries and subsequently select antibodies of the alpha isotype against a wide variety of pathogens and parasites.
Wieland
Willemien H
WH
Laboratory of Molecular Recognition and Antibody Technology, Wageningen University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands.
Orzáez
Diego
D
Lammers
Aart
A
Parmentier
Henk K
HK
Schots
Arjen
A
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors; D007070:Immunoglobulin A / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000276:immunology; D008969:Molecular Sequence Data; D019151:Peptide Library; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
D000913:Antibodies, Protozoan; D007070:Immunoglobulin A; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D011994:Recombinant Proteins