Palleronia marisminoris gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium belonging to the 'Alphaproteobacteria', isolated from a saline soil.
Strain B33(T) is a moderately halophilic, exopolysaccharide-producing, Gram-negative, non-motile rod isolated from a hypersaline soil bordering a saline saltern on the Mediterranean seaboard in Murcia (Spain). The bacterium is chemoheterotrophic and strictly aerobic. It contains a pink pigment but does not synthesize bacteriochlorophyll a. It requires 0.66 M Na+, 0.1 M Mg2+ and 0.1 M K+ for optimum growth. It does not produce acid from carbohydrates. It cannot grow with carbohydrates, organic acids, sugars, alcohols or amino acids as sole sources of carbon and energy. Its major fatty-acids are 18 : 1omega7c (68.9 %) and 19 : 0 cyclo omega8c (12.8 %). The sole respiratory lipoquinone found in strain B33T is ubiquinone-10. The DNA G+C content is 64.2 mol%. 16S rRNA gene sequence comparisons show that the isolate is a member of the Roseobacter clade within the class 'Alphaproteobacteria'. The similarity values with Roseivivax halodurans and Roseivivax halotolerans are 88.2 and 88.0 % respectively and 92.2 % with Salipiger mucosus. DNA-DNA hybridization values with these species are <30 %. In the light of the polyphasic evidence gathered in this study it is proposed that the isolate be classified as a novel genus and species with the name Palleronia marisminoris gen. nov., sp. nov. The proposed type strain is strain B33T (=CECT 7066T=LMG 22959T).
Martínez-Checa
Fernando
F
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain.
Quesada
Emilia
E
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain.
Martínez-Cánovas
M José
MJ
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain.
Llamas
Inmaculada
I
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain.
Béjar
Victoria
V
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain.
D025541:Bacteriochlorophyll A / Q000032:analysis; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011188:Potassium / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D012964:Sodium / Q000378:metabolism*; D012988:Soil Microbiology
D025541:Bacteriochlorophyll A; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011135:Polysaccharides, Bacterial; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012462:Saline Solution, Hypertonic; D012964:Sodium; D011188:Potassium