[Usefulness of serum inhibin B measurement for evaluation of ovarian reserve in controlled ovarian hyperstimulation].
OBJECTIVE
To assess usefulness of serum inhibin B (INHB) measurement for evaluation of ovarian function.


METHODS
Serum INHB level on day 3 of menstrual cycle was determined by enzyme labeled immunosorbent assay (ELISA) in 96 cases of in vitro fertilization and embryo transfer (IVF-ET). The patients were classified into 3 groups including (1) poor response (n = 6), normal response (n = 72), and over response (n = 18) according to their response to ovary stimulation. In addition, serum follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E(2)) levels were also determined by chemiluminescent microparticle immunoassay in these patients. Evaluation of the INHB measurement and related factors was performed by statistical analysis.


RESULTS
Serum INHB levels in poor, normal and over-response groups were (28 +/- 20), (85 +/- 42), (92 +/- 34) pg/ml; FSH levels were (11.9 +/- 5.3), (7.5 +/- 2.6), (7.2 +/- 1.7) U/L; E(2) levels on day 3 of human chorionic gonadotropin (hCG) administration were (2558 +/- 2108), (9366 +/- 4472), (18 392 +/- 9655) pmol/L; numbers of retrieved oocytes per cycle were (0.6 +/- 0.4), (8.7 +/- 3.6), (14.3 +/- 2.9); top grade embryos were (0.4 +/- 0.3), (3.8 +/- 1.9), (4.6 +/- 1.7); pregnancy rates were 16.7%, 36.1%, 61.1%, respectively. INHB level was negatively correlated to FSH (r = -0.222, P < 0.05) and FSH/LH (r = -0.371, P < 0.05); while positively correlated to E(2) on the day of hCG administration (r = 0.336, P < 0.05), number of retrieved oocytes (r = 0.404, P < 0.05), number of quality embryos (r = 0.323, P < 0.05) and pregnancy rate (r = 0.246, P < 0.05), respectively.


CONCLUSIONS
INHB test may reflect the ovarian reserve which is of clinic importance in the guidance of controlled ovarian hyperstimulation.
Wu
Ri-ran
RR
Reproductive Medical Center, Zhongshan Boai Hospital, Zhongshan, Guangdong Province 528403, China.
Liao
Yue-chan
YC
Ye
Yun
Y
Du
Yan
Y
Ke
Wan-na
WN
Zhang
Xu-bin
XB
Zhuang
Guang-lun
GL
D000328:Adult; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004624:Embryo Transfer; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000628:therapy; D007265:Inhibins / Q000097:blood*; D007986:Luteinizing Hormone / Q000097:blood; D010053:Ovary / Q000187:drug effects / Q000502:physiology*; D011237:Predictive Value of Tests; D011247:Pregnancy; D018873:Pregnancy Rate; D013480:Superovulation
D006063:Chorionic Gonadotropin; C422364:inhibin B; D004958:Estradiol; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone