Degradation of 2,6-di-tert-butylphenol by an isolated high-efficiency bacterium strain.
An aerobic bacterium strain, F-3-4, capable of effectively degrading 2, 6-ditert-butylphenol (2, 6-DTBP), was isolated and screened out from an acrylic fiber wastewater and the biofilm in the wastewater treatment facilities. This strain was identified as Alcaligenes sp. through morphological, physiological and biochemical examinations. After cultivation, the strain was enhanced by 26.3% in its degradation capacity for 2,6-DTBP. Results indicated that the strain was able to utilize 2,6-DTBP, lysine, lactamine, citrate, n-utenedioic acid and malic acid as the sole carbon and energy source, alkalinize acetamide, asparagine, L-histidine, acetate, citrate and propionate, but failed to utilize glucose, D-fructose, D-seminose, D-xylose, serine and phenylalanine as the sole carbon and energy source. The optimal growth conditions were determined to be: temperature 37 degrees C, pH 7.0, inoculum size 0.1% and shaker rotary speed 250 r/min. Under the optimal conditions, the degradation kinetics of 2,6-DTBP with an initial concentration of 100 mg/L was studied. Results indicated that 62.4% of 2,6-DTBP was removed after 11 d. The degradation kinetics could be expressed by Eckenfelder equation with a half life of 9.38 d. In addition, the initial concentration of 2,6-DTBP played an important role on the degradation ability of the strain. The maximum initial concentration of 2,6-DTBP was determined to be 200 mg/L. Above this level, the strain was overloaded and exhibited significant inhibition.
Zhang
Ya-lei
YL
Key Laboratory of Yangtze Water Environment of Ministry of Education, Tongji University, Shanghai 200092, China. zyl@mail.tongji.edu.cn
Fang
Zhen-wei
ZW
Xu
De-qiang
DQ
Xiao
Yi-ping
YP
Zhao
Jian-fu
JF
Qiang
Zhi-min
ZM
D000421:Alcaligenes / Q000378:metabolism*; D001673:Biodegradation, Environmental; D050260:Carbohydrate Metabolism / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010636:Phenols / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
D010636:Phenols; D014874:Water Pollutants, Chemical; C035407:2,6-di-tert-butylphenol