The effect of sugars and free amino acids from the freshwater prawn Macrobrachium rosenbergii hemolymph on lectin activity and on oxidative burst.
We determined the effect of low molecular weight components (LMWC) from healthy juvenile and adult Macrobrachium rosenbergii hemolymph on lectin activity and oxidative burst (OB) in hemocytes. In an attempt to identify the LMWC that affect the lectin's hemagglutinating activity or oxidative burst, we determined the hemolymph carbohydrates and free amino acids (FAA) concentration. The LMWC (<2000 Da) were obtained after dialysis of the hemolymph. Our results showed that LMWC from juveniles exerted a greater inhibition on lectin than LMWC from adult hemolymph. Production of superoxide radicals by hemocytes was lower in the presence of juvenile (p<0.05) as compared to adult LMWC. FAA composition of the hemolymph and of LMWC from adults showed higher proportion of alanine (which corresponded to 25% of total FAA) and proline (>20%); whereas, in juveniles, the main FAA identified were glycine (>40%) and alanine (26%). N-Acetyl-D-glucosamine (GlcNAc) was the main sugar residue in the hemolymph and LMWC from juveniles; its concentration was 2.4 times higher than glucose (Glc), whereas, in adults, Glc was the main free sugar residue. Our results suggest that the proportion of FAA and carbohydrates in the hemolymph of M. rosenbergii seems to be correlated with the maturation process; furthermore, the high proportion of free GlcNAc and glycine regulate, in the juvenile stage, lectin activity and cellular oxidative mechanisms, respectively.
Soria
Frida
F
Laboratorio de Lectinas, CIQ, Universidad Autónoma del Estado de Morelos, Cuernavaca Morelos 62210, Mexico.
Sierra
Claudia
C
Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, Calzada de Tlalpan 4502, 01040, Mexico.
Bouquelet
Stephane
S
Laboratoire de Chimie Biologique de la Université des Sciences et Technologies de Lille, UMR du CNRS no. 8576, Villeneuve d'Ascq 59655, France.
Brassart
Colette
C
Laboratoire de Chimie Biologique de la Université des Sciences et Technologies de Lille, UMR du CNRS no. 8576, Villeneuve d'Ascq 59655, France.
Agundis
Concepción
C
Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina, UNAM 04510, Mexico.
Zenteno
Edgar
E
Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina, UNAM 04510, Mexico.
Vázquez
Lorena
L
Laboratorio de Lectinas, CIQ, Universidad Autónoma del Estado de Morelos, Cuernavaca Morelos 62210, Mexico. Electronic address: Ivazquez@mklinux.dti.uaem.mx.
D000596:Amino Acids / Q000097:blood* / Q000737:chemistry; D000818:Animals; D002241:Carbohydrates / Q000097:blood* / Q000737:chemistry; D004912:Erythrocytes / Q000502:physiology; D005618:Fresh Water; D006384:Hemagglutination; D006434:Hemocytes / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D033541:Palaemonidae / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016897:Respiratory Burst; D013481:Superoxides / Q000378:metabolism
D000596:Amino Acids; D002241:Carbohydrates; D037102:Lectins; D013481:Superoxides