Shedding of feline leukemia virus RNA in saliva is a consistent feature in viremic cats.
The purpose of this investigation was to characterize the shedding pattern of feline leukemia virus (FeLV) RNA in saliva, and to correlate it with the proviral load in whole blood, viral load in plasma, levels of p27 in saliva and plasma, the isolation of infectious FeLV from saliva, and the titers of FeLV-specific antibodies of the IgG and IgA isotypes. We evaluated 24 experimentally FeLV-infected cats for these parameters using real-time RT-PCR and PCR, cell culture assay and sandwich ELISA. We observed that shedding of viral RNA in saliva was a consistent feature in viremic cats. Latently FeLV-infected cats, displaying a very low proviral load, did not shed infectious virus in saliva, but occasionally shed viral RNA. Consequently, salivary shedding of FeLV RNA may not necessarily indicate a transmission potential for susceptible cats. This study also confirmed previous results from our laboratory, showing that a negative result for p27 in plasma, or for viral RNA in plasma or saliva does not exclude FeLV infection, considering that blood cells from those cats contained provirus. We also showed that FeLV RNA and DNA were stable for more than 64 days in saliva samples stored at room temperature. We conclude that the detection of FeLV RNA in saliva may be a useful indicator of viremia, and that the detection of salivary viral RNA by RT-PCR could become a reliable tool for the diagnosis of FeLV infection, which is facilitated by the low invasive method of collection of the samples.
Gomes-Keller
M A
MA
Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057, Zurich, Switzerland. mgomes@vetclinics.unizh.ch
Tandon
R
R
Gönczi
E
E
Meli
M L
ML
Hofmann-Lehmann
R
R
Lutz
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005256:Leukemia Virus, Feline / Q000276:immunology / Q000302:isolation & purification*; D016582:Leukemia, Feline / Q000175:diagnosis* / Q000821:virology; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011533:Proviruses / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012463:Saliva / Q000821:virology*; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors; D019562:Viral Load / Q000662:veterinary; D017735:Virus Latency; D017201:Virus Shedding
D000914:Antibodies, Viral; D012367:RNA, Viral