title: Intratracheal and intranasal immunization with ovalbumin conjugated with Bacillus firmus as a carrier in mice.
abstract: Inactivated Bacillus firmus (BF), G+ nonpathogenic bacterium of the external environment, was coupled to ovalbumin (OVA) and used in immunization experiments as antigen carrier. Balb/c mice were immunized thrice intra-tracheally and intra-nasally with conjugates of OVA and BF. Surprisingly, administration of OVA-BF conjugates inhibited anti-OVA IgG response in both sera and mucosal secretions if compared to an exposure to OVA alone. The suppression of antigen-specific antibody production was accompanied by promotion of TH1 phenotype.
authors.affiliation: Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, Prague, Czechia.
authors.forename: P
authors.initials: P
authors.lastname: Mlcková
authors.forename: M
authors.initials: M
authors.lastname: Polácek
authors.forename: D
authors.initials: D
authors.lastname: Cechová
authors.forename: L
authors.initials: L
authors.lastname: Marusková
authors.forename: I
authors.initials: I
authors.lastname: Stanková
authors.forename: P
authors.initials: P
authors.lastname: Chalupná
authors.forename: O
authors.initials: O
authors.lastname: Novotná
authors.forename: J
authors.initials: J
authors.lastname: Julák
authors.forename: L
authors.initials: L
authors.lastname: Prokesová
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D000941:Antigens / Q000008:administration & dosage*; D001407:Bacillus / Q000276:immunology*; D004337:Drug Carriers; D005260:Female; D018928:Immunity, Mucosal; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D014132:Trachea
chemical_list: D000907:Antibodies, Bacterial; D000941:Antigens; D004337:Drug Carriers; D007074:Immunoglobulin G; D010047:Ovalbumin