[Purification and activities of an alkaline protein from mushroom Coprinus comatus].
An Alkaline protein, y3, can be purified from the fruiting bodies of mushroom Coprinus comatus by means of CM-sepharose FF ion-exchange column chromatography and Superdex 75 High Resolution molecular sieve chromatography. The protein has a molecular weight of about 14.4kD by SDS-PAGA. Some activities of y3 have been detected, and the result is the following: the inhibition rate against Tobacco Mosaic Virus is 83.0% when the concentration of y3 is 12.5 microg/ mL. y3 is able to agglutinate rabbit and human erythrocytes at the concentration of 1.562 microg/mL and 0.781 microg/mL. Using an assay system based on mach cancer cell line MGC-803, y3 was studied for its inhibitory ability against celles multiplication, and the IC50 is 12 microg/mL. The N-terminal sequence is NRDVAACARFIDDFCDTLTP, which has no homology with other sequences in Genbank.
Wu
Liping
L
Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. wuliping@fjau.edu.cn
Wu
Zujian
Z
Lin
Qiying
Q
Xie
Lianhui
L
D000818:Animals; D003302:Coprinus / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006388:Hemagglutinins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D008970:Molecular Weight; D011817:Rabbits; D014027:Tobacco Mosaic Virus / Q000187:drug effects
D005656:Fungal Proteins; D006388:Hemagglutinins