Assessment of reproductive toxicity in male rats following acute and sub-chronic exposures to diphenyl diselenide and diphenyl ditelluride.
The present study was conducted to evaluate the toxicity of the exposure to diphenyl diselenide [(PhSe)2] and diphenyl ditelluride [(PhTe)2] on reproductive system in Wistar rats. Adult male rats were exposed intraperitonealy (acute) or subcutaneously (sub-chronic, during 4 or 8 weeks) to (PhSe)2 or (PhTe)2 prior to mating. A number of biochemical parameters in rat testes were examined, such as delta-aminolevulinate dehydratase (delta-ALA-D) activity, lipid peroxidation, glycogen content and components of the antioxidant defenses (superoxide dismutase (SOD) activity and ascorbic acid concentration). Furthermore, a possible effect on fertility and reproductive performance in male rats were studied. Sperm counts of caudal epididymis were also evaluated. No lethality was noted in any group. Reduction on body weight in rats which received (PhTe)2 was only evidenced in acute exposure, while (PhSe)2-exposed rats presented significant loss of body weight in acute and 4 week-exposure. Mating and fertility indexes were not affected after acute and sub-chronic exposure. Regarding other parameters studied, except for a decrease in testes glycogen content in acutely (PhSe)2-treated group, no alterations were found in treated groups. Sperm counts of rats treated acutely and sub-chronically were unaffected by drugs exposure. Histological evaluation revealed no modification on testicular tissue in rats exposed to (PhSe)2 and (PhTe)2. The results suggest the absence of the male reproductive toxicity induced by (PhSe)2 and (PhTe)2 administered intraperitonealy (acute) or subcutaneously (sub-chronical) to adult rats Wistar.
Stangherlin
Eluza Curte
EC
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, SM, RS, CEP 97105-900 Santa Maria, Brazil.
Favero
Alexandre Marafon
AM
Weis
Simone Nardin
SN
Zeni
Gilson
G
Rocha
João Batista Teixeira
JB
Nogueira
Cristina Wayne
CW
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001555:Benzene Derivatives / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D005260:Female; D005298:Fertility / Q000187:drug effects*; D006003:Glycogen / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009942:Organometallic Compounds / Q000633:toxicity*; D016566:Organoselenium Compounds / Q000633:toxicity*; D000623:Porphobilinogen Synthase / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects*; D013076:Sperm Count; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D023382:Toxicity Tests, Acute; D036401:Toxicity Tests, Chronic
D001555:Benzene Derivatives; D009942:Organometallic Compounds; D016566:Organoselenium Compounds; D017392:Thiobarbituric Acid Reactive Substances; C434247:diphenylditelluride; C061132:diphenyldiselenide; D006003:Glycogen; D013482:Superoxide Dismutase; D000623:Porphobilinogen Synthase; D001205:Ascorbic Acid