[Cold storage of platelet suspension by adding trehalose].
The study was aimed to explore the trehalose method for storing platelets in cold. (51)Cr-labeling platelet was used to detect the platelet survival. The platelet function in vitro was performed by platelet aggregate analyzer. After treatment with 50 mg/ml trehalose at 37 degrees C for 4 hours, the rabbit platelet concentrates (PC, 2.0 x 10(9)/ml) were stored in 4-8 degrees C refrigeration, the platelet function in vitro and survival of chilled platelets transfused into self-rabbits were observed. The results showed that trehalose could protect the chilled rabbit platelets. After PC stored at 20-24 degrees C and 4-8 degrees C for up to 24 hours, the platelet aggregate in vitro in response to 11.2 micromol/L ADP were (75.3 +/- 9.8)% and (80.5 +/- 12.5)%, the survival of PC stored at 20-24 degrees C and 4-8 degrees C for 24, 48, 72 hours after transfused into self-rabbits were (78.1 +/- 7.9)%, (65.4 +/- 6.7)%, (57.5 +/- 7.2)% and (5.1 +/- 2.5)%, (2.8 +/- 2.0)%, (0.9 +/- 0.8)%, respectively. The PC treated with 50 mg/ml trehalose were remained stable for up to 12 days of refrigerated storage in autologous plasma. The platelet aggregate in vitro in response to 11.2 micromol/L ADP at 12 days after stored in refrigeration was (77.8 +/- 9.5)%, the survival on 24, 48, 72 hours of platelet transfused into self-rabbits were (75.7 +/- 11.0)%, (67.0 +/- 8.5)%, (56.8 +/- 8.0)%, respectively. Compared with control group of storing at 20-24 degrees C for 24 h, P > 0.05. In conclusion, trehalose can protect the chilled blood platelets, prolong the circulation of refrigerated rabbit platelets, and not impair chilled rabbit platelet function.
Huang
Cheng-Yin
CY
Department of Transfusion Blood, Jiangsu Province Blood Center, Nanjing 210042, China. Huangchengyin1223@126.com
Tang
Rong-Cai
RC
Cai
Li
L
Cui
Yun
Y
Liu
Lu
L
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects*; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D017713:Platelet Transfusion; D011817:Rabbits; D014199:Trehalose / Q000494:pharmacology*
D003451:Cryoprotective Agents; D000244:Adenosine Diphosphate; D014199:Trehalose