Synthetic, site-specific biotinylated analogs of human MCP-1.
Human monocyte chemoattractant protein 1 (MCP-1, CCL2) is a 8.6-kDa protein that has been implicated in a number of diseases including atherosclerosis, rheumatoid arthritis, chronic obstructive pulmonary disease and cancer. As part of a program to identify antibodies against MCP-1, we synthesized site-specific, biotinylated human MCP-1 analogs to be used for panning of an antibody phage display library. In contrast to material obtained from random biotinylation, the site-specific biotinylated analogs were homogeneous and retained full activity.
Kruszynski
Marian
M
Centocor R&D, Inc., 145 King of Prussia Road, Radnor, PA 19087, USA. mkruszy2@cntus.jnj.com
Tsui
Ping
P
Stowell
Nicole
N
Luo
Jinquan
J
Nemeth
Jennifer F
JF
Das
Anuk M
AM
Sweet
Raymond
R
Heavner
George A
GA
D000595:Amino Acid Sequence; D019921:Biotinylation; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D018932:Chemokine CCL2 / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D018932:Chemokine CCL2; D002118:Calcium