title: Differences in mitochondrial genome organization of Cryptococcus neoformans strains.
abstract: The organization of the mitochondrial genomes in two strains belonging in different varieties of Cryptococcus neoformans was analysed. Physical maps of the mtDNA of the IFM5844 (var. neoformans) and IFO410 (var. grubii) strains were constructed by using EcoRI and EcoRV restriction enzymes; functional maps were constructed by hybridization, cloning and sequencing. Most of the genes important in the mitochondrial function (ND1, ND2, ND3, ND4, ND4L, ND5, ND6, ATP6, ATP9, COX1, COX2 and COB) and protein synthesis (SsrRNA and LsrRNA) were localized. We did not find any differences between the strains in the order of these genes. However, they differed significantly in the sizes of the mtDNAs: 32.6 kb for IFM5844, and 24.1 kb for IFO410. This can be attributed to two large regions of the mtDNA. In these regions, differences were found in the numbers of introns in COX1 (no intron in var. grubii, 5 introns in var. neoformans), COB (1 intron in var. grubii, 2 introns in var. neoformans), LsrRNA (no intron in var. grubii, 2 introns in var. neoformans), and ND5 (no intron in var. grubii, 1 intron in var. neoformans) genes. In several introns of the COB and COX1 genes LAGLIDADG motifs were found. Differences were also observed in the nucleotide sequences of some genes and in the sizes and sequences of intergenic regions. The nucleotide sequences of the genes of the IFM and IFO strains were compared with those of the H-99 and JEC 21 strains from the database. Surprisingly high similarities were found between the strains belonging in var. grubii (IFO 410 and H-99) and var. neoformans (IFM 5844 and JEC 21).
authors.affiliation: Department of Microbiology, Faculty of Sciences, University of Szeged, P.O. Box 533, Szeged H-6701, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Litter
authors.forename: Andrea
authors.initials: A
authors.lastname: Keszthelyi
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Hamari
authors.forename: Ilona
authors.initials: I
authors.lastname: Pfeiffer
authors.forename: Judit
authors.initials: J
authors.lastname: Kucsera
mesh_terms: D003001:Cloning, Molecular; D003455:Cryptococcus neoformans / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D021901:DNA, Intergenic; D004272:DNA, Mitochondrial / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D023061:Gene Order; D005800:Genes, Fungal; D007438:Introns; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic; D004272:DNA, Mitochondrial; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases