The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms.
The mechanisms of viral diseases are a major focus of biology. Despite intensive investigations, how a plant virus interacts with host factors to cause diseases remains poorly understood. The Rice dwarf virus (RDV), a member of the genus Phytoreovirus, causes dwarfed growth phenotypes in infected rice (Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector. However, its role during RDV infection within the rice host is unknown. By yeast two-hybrid and coimmunoprecipitation assays, we report that P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. Furthermore, the expression of ent-kaurene oxidases was reduced in the infected plants. The level of endogenous GA1 (a major active gibberellin in rice vegetative tissues) in the RDV-infected plants was lower than that in healthy plants. Exogenous application of GA3 to RDV-infected rice plants restored the normal growth phenotypes. These results provide evidence that the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression. In addition, the interaction between P2 and rice ent-kaurene oxidase-like proteins may decrease phytoalexin biosynthesis and make plants more competent for virus replication. Moreover, P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development.
Zhu
Shifeng
S
Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China.
Gao
Feng
F
Cao
Xuesong
X
Chen
Mao
M
Ye
Gongyin
G
Wei
Chunhong
C
Li
Yi
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D036022:Capsid Proteins / Q000502:physiology*; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D005875:Gibberellins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology; D012087:Reoviridae / Q000472:pathogenicity* / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D036022:Capsid Proteins; D018744:DNA, Plant; D005875:Gibberellins; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; C066398:ent-kaurene oxidase