[Immunogenicity of DNA vaccine expressing GP5 of porcine reproductive and respiratory syndrome virus fused with VP22 of bovine herpesvirus 1].
To enhance the immuogenicity of DNA vaccines expressing the GP5 protein of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the tegument protein VP22 (encoded by VP22 gene) of Bovine Herpesvirus 1 (BHV-1), which has been demonstrated to exhibit the unusual protein transduction property, was fused to N-terminus of GP5 of DNA vaccine construct pCI-ORF5M, resulting in pCI-VP22-ORF5M expressing VP22-GP5 fusion protein. The expression of VP22-GP5 fusion protein was confirmed by both indirect immunofluorescence assay (IFA) and Western blot. To investigate its immunogenicity, BALB/c mice were immunized with the fusion expression plasmid pCI-VP22-ORF5M and non-fusion expression plasmid pCI-ORF5M, respectively. The GP5-specific ELISA antibodies, neutralizing antibodies and lymphocyte proliferative responses were evaluated at various time points after primary immunization. The results showed that GP5-specific ELISA antibodies, neutralizing antibodies, and lymphocyte proliferative responses induced by DNA vaccine pCI-VP22-ORF5M were higher significantly than those of DNA vaccine pCI-ORF5M, indicating that fusion expression with BHV-1 VP22 significantly enhances the immuogenicity of DNA vaccine expressing the PRRSV GP5 protein, and that this strategy may also be useful to develop more efficient DNA vaccines against other pathogens.
Zhao
Wu
W
Laboratory of Animal Virology, College of Animal Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Xiao
Shao-Bo
SB
Fang
Liu-Rong
LR
Jiang
Yun-Bo
YB
Song
Yun-Feng
YF
Yan
Lin
L
Yu
Xiao-Lan
XL
Chen
Huan-Chun
HC
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D019677:Artificial Gene Fusion; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D019318:Porcine Reproductive and Respiratory Syndrome / Q000517:prevention & control; D011897:Random Allocation; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
D000956:Antigens, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C114789:glycoprotein 5, PRRSV; C434023:tegument protein VP22, bovine herpesvirus 1