Detection and characterization of protein nitrosothiols.
Protein S-nitrosylation is a post-translational modification of cysteine residues elicited by nitric oxide (NO). Detection and quantification of protein nitrosothiols remains a challenge because of the lability of the nitrosothiol moiety. Here, we describe approaches for labeling S-nitrosylated proteins with affinity and radioactive tags to facilitate their detection, purification, and identification.
Jaffrey
Samie R
SR
Department of Psychology, Weill Medical College, Cornell University, New York, NY 10021, USA.
D011506:Proteins / Q000737:chemistry*; D026403:S-Nitrosothiols / Q000032:analysis* / Q000737:chemistry*
D011506:Proteins; D026403:S-Nitrosothiols