Inhibitory effect of Aucubin isolated from Eucommia ulmoides against UVB-induced matrix metalloproteinase-1 production in human skin fibroblasts.
Of 30 herbal plants tested, the methanol extracts of Eucommia ulmoides (52%), Evodia officinalis (45%), and Pleuropterus multiflorus (41%) each showed a potent inhibitory effect on the matrix metalloproteinase-1 (MMP-1) production in ultraviolet B (UVB)-irradiated human fibroblasts. Aucubin was isolated as the MMP-1 inhibitor from E. ulmoides, and significantly suppressed the production of MMP-1 by nearly 57% compared to the control. It also reduced MMP-1 mRNA expression. These results suggest that aucubin is a photoprotective phytochemical, and could be used as a potential agent in preventing photoaging.
Ho
Jin-Nyoung
JN
Department of Food Technology, Graduate School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Lee
Yoo-Hyun
YH
Lee
Yun-Dong
YD
Jun
Woo-Jin
WJ
Kim
Hye-Kyung
HK
Hong
Bum-Shik
BS
Shin
Dong-Hoon
DH
Cho
Hong-Yon
HY
D031283:Eucommiaceae / Q000737:chemistry*; D005347:Fibroblasts / Q000201:enzymology* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D057888:Iridoid Glucosides; D039823:Iridoids / Q000302:isolation & purification / Q000494:pharmacology*; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D010936:Plant Extracts; D012333:RNA, Messenger / Q000032:analysis; D011837:Radiation-Protective Agents / Q000302:isolation & purification / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D014466:Ultraviolet Rays
D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D010936:Plant Extracts; D012333:RNA, Messenger; D011837:Radiation-Protective Agents; C006650:aucubin; D020781:Matrix Metalloproteinase 1