title: Determination of vitreous interleukin-1 (IL-1) and tumour necrosis factor (TNF) levels in proliferative diabetic retinopathy.
abstract: We measured interleukin-1 beta (IL-1beta) and tumour necrosis factor-alpha (TNF-alpha) in the vitreous humour and serum of patients with proliferative diabetic retinopathy (PDR), in order to determine the role of these cytokines in the pathogenesis of the disease. Vitreous and serum samples were collected from 21 patients with PDR who were undergoing pars plana vitrectomy. Control vitreous samples were obtained from cadavers and control serum samples from healthy subjects. The cytokines were measured by enzyme-linked immunosorbent assay. Vitreous IL-1beta and TNF-alpha concentrations in patients with PDR exceeded those of controls (P<0.05), as did serum IL-1beta and TNF-alpha. We suggest that increased vitreous IL-1beta and TNF-alpha levels may play a significant role in the pathogenesis of PDR, which features abnormal cell proliferation and neovascularisation.
authors.affiliation: Department of Ophthalmology, Cukurova University, Faculty of Medicine, Adana-Turkey, Balcali, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Demircan
authors.forename: B G
authors.initials: BG
authors.lastname: Safran
authors.forename: M
authors.initials: M
authors.lastname: Soylu
authors.forename: A A
authors.initials: AA
authors.lastname: Ozcan
authors.forename: S
authors.initials: S
authors.lastname: Sizmaz
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003930:Diabetic Retinopathy / Q000097:blood / Q000276:immunology* / Q000601:surgery; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis* / Q000097:blood; D008297:Male; D008875:Middle Aged; D015861:Retinal Neovascularization / Q000097:blood / Q000276:immunology* / Q000601:surgery; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*; D014821:Vitrectomy; D014822:Vitreous Body / Q000276:immunology
chemical_list: D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha