[Inter-laboratory study for validation of a Japanese official analytical method for determination of patulin in apple juice].
To validate a modified version of AOAC official method of analysis 995.10 as an official standard in Japan for determination of patulin in apple juice, an inter-laboratory study was performed in 11 laboratories using a non-contaminated sample, 2 naturally contaminated samples and 2 spiked samples of apple juice. For naturally contaminated apple juices, the relative standard deviations for repeatability and reproducibility were 3.2, 7.1% and 10.0, 21.7%, respectively. HORRAT values were 0.4, 0.9. The average recovery of patulin from spiked sample was 83.7%. The limit of quantification was calculated as 10 microg/kg. From these results, the method was thought to be suitable as an official standard for determination of patulin in apple juice in Japan.
Sugita-Konsihi
Yoshiko
Y
National Institute of Health Sciences, Setagaya-ku, Tokyo, Japan.
Tanaka
Toshitugu
T
Sugiura
Yoshitugu
Y
Tabata
Setsuko
S
Nakajima
Masahiro
M
Sakurai
Hiroyuki
H
Nakaie
Yoko
Y
Sato
Koichi
K
Kitani
Yusuke
Y
Fujita
Kazuhiro
K
Hayashi
Sachiko
S
Iizuka
Tayoshi
T
Hirakawa
Yoshinori
Y
Mochizuki
Naoki
N
Hoshino
Mariko
M
Sato
Yoshirou
Y
Takahashi
Naomi
N
Takatori
Kosuke
K
D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000592:standards; D007564:Japan; D027845:Malus / Q000737:chemistry*; D010365:Patulin / Q000032:analysis*
D010365:Patulin