title: Vibrio gigantis sp. nov., isolated from the haemolymph of cultured oysters (Crassostrea gigas).
abstract: Polyphasic analysis of four new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on DNA gyrase subunit B (gyrB), RNA polymerase sigma70 factor (rpoD), replication origin-binding protein (rctB) and transmembrane regulatory protein (toxR) genes, fluorescent amplified fragment length polymorphism and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed that these new isolates should be accommodated in a novel species, Vibrio gigantis sp. nov. Phenotypic features that differentiate V. gigantis from other known Vibrio species include arginine dihydrolase, gelatinase and beta-galactosidase activities, NO(2) production, growth at 35 degrees C, and utilization of sucrose, melibiose, amygdalin, glycerol, galactose, starch and glycogen. The type strain is LGP 13T (=LMG 22741T=CIP 108656T).
authors.affiliation: Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
authors.forename: F Le
authors.initials: FL
authors.lastname: Roux
authors.affiliation: Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
authors.forename: A
authors.initials: A
authors.lastname: Goubet
authors.affiliation: Laboratory for Microbiology and BCCMTM/LMG Bacteria Collection, Laboratory for Microbiology, Ghent University, K.L. Ledeganckstraat 35, Ghent 9000, Belgium.
authors.forename: F L
authors.initials: FL
authors.lastname: Thompson
authors.affiliation: Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
authors.forename: N
authors.initials: N
authors.lastname: Faury
authors.affiliation: Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
authors.forename: M
authors.initials: M
authors.lastname: Gay
authors.affiliation: Laboratory for Microbiology and BCCMTM/LMG Bacteria Collection, Laboratory for Microbiology, Ghent University, K.L. Ledeganckstraat 35, Ghent 9000, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Swings
authors.affiliation: Laboratoire de Génétique et Pathologie, Institut français de recherche pour l'exploitation de la mer (Ifremer), 17390 La Tremblade, France.
authors.forename: D
authors.initials: D
authors.lastname: Saulnier
mesh_terms: D000818:Animals; D049896:Crassostrea / Q000382:microbiology*; D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000145:classification* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase