Polyomavirus EGFP-pseudocapsids: analysis of model particles for introduction of proteins and peptides into mammalian cells.
A vector for preparation of mouse polyomavirus capsid-like particles for transfer of foreign peptides or proteins into cells was constructed. Model pseudocapsids carrying EGFP fused with the C-terminal part of the VP3 minor protein (EGFP-VLPs) have been prepared and analysed for their ability to be internalised and processed by mouse cells and to activate mouse and human dendritic cells (DC) in vitro. EGFP-VLPs entered mouse epithelial cells, fibroblasts and human and mouse DC efficiently and were processed by both, lysosomes and proteasomes. Surprisingly, they did not induce upregulation of DC co-stimulation molecules or maturation markers in vitro; however, they did induce interleukin 12 secretion.
Boura
E
E
Genetics and Microbiology, Faculty of Science, Charles University in Prague, Vinicná 5, 128 44 Prague 2, Czech Republic.
Liebl
D
D
Spísek
R
R
Fric
J
J
Marek
M
M
Stokrová
J
J
Holán
V
V
Forstová
J
J
D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D003713:Dendritic Cells / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D018664:Interleukin-12 / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D010455:Peptides / Q000235:genetics*; D011120:Polyomavirus / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D014161:Transduction, Genetic / Q000379:methods*; D014771:Virion / Q000235:genetics
D036022:Capsid Proteins; D010455:Peptides; D011506:Proteins; D049452:Green Fluorescent Proteins; D018664:Interleukin-12