[Experimental study of cisplatin loaded polylactic acid-polyethylene glycol nano-particles for targeting oral carcinoma].
OBJECTIVE
To investigate the target deliver of Cisplatin to oral carcinoma tissues by intravenous injection of Cisplatin loaded polylactic acid- polyethylene glycol nanoparticles (CDDP-PLA-PEG-NP).


METHODS
CDDP-PLA-PEG-NP was prepared by the emulsion-solvent evaporation method. The buccal cancer model was established in 64 golden hamsters, which were divided randomly into two groups for 32 animals in each group, CDDP-PLA-PEG-NP (6.6 mg/kg) and CDDP (1 mg/kg) were respectively injected into mice tail vein. At 0.083, 0.5, 1, 2, 4, 6, 12, 24 h after drug administration. 4 animals in each group were sacrificed and CDDP concentration in the plasma and tumor were determined by high performance liquid chromatography. Targeting ability was evaluated by targeting index (TI), selectivity index (SI) and relative extraction efficiency (re).


RESULTS
The average diameter of CDDP-PLA-PEG-NP was (143.2 +/- 1.8) nm. The diameter distribution was from 103.5 nm to 175.8 nm. Drug loading and embedding ratio were (15.2 +/- 0.9) %, (89.0 +/- 0.8) % respectively. Values of TI and SI are more than 1 at 8 time points. The area under CDDP concentration-time curve of oral carcinoma tissues in CDDP-PLA-PEG-NP group was 10.36 times as many as that in CDDP group.


CONCLUSIONS
CDDP-PLA-PEG-NP can specifically deliver CDDP to oral carcinoma tissues by vein injection. Stealth anticancer nano-particle system can be regarded as a valuable drug deliver system to treat oral carcinoma.
Yang
Kai
K
Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China.
Chen
Shao-wei
SW
Chen
Rui
R
Wan
Yu-ming
YM
D000818:Animals; D002945:Cisplatin; D016503:Drug Delivery Systems; D019344:Lactic Acid; D051379:Mice; D009062:Mouth Neoplasms; D053758:Nanoparticles; D009369:Neoplasms; D010316:Particle Size; D011091:Polyesters; D011092:Polyethylene Glycols; D011108:Polymers
D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); D002945:Cisplatin