[Isolation of a bacterium producing epoxide hydrolase with high enantioselectivity and optimization of fermentation conditions].
A bacterial strain 463# producing epoxide hydrolase was isolated from oil-contaminated soils. It showed high enantioselectivity towards styrene oxide, and also towards glycidates, such as ethyl phenyl glycidate and ethyl benzyl glycidate. The strain was identified as Bacillus sp. The fermentation conditions for this strain were optimized. With 1% glucose as carbon source, 1% (NH4)2SO4 and 0.5% beef extract as nitrogen source, Bacillus sp. 463 grew well and showed high epoxide hydrolase activity. In addition, 2-10 mmol/L styrene oxide could promote the production of epoxide hydrolase.
Li
Congfa
C
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
Cui
Hongjie
H
Tian
Xiuyun
X
Ji
Aiguo
A
Qu
Yinbo
Y
D001407:Bacillus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D004851:Epoxide Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D012988:Soil Microbiology; D013379:Substrate Specificity
D001426:Bacterial Proteins; D003470:Culture Media; D004851:Epoxide Hydrolases