title: Methacrylate-based chromatographic media.
abstract: This review summarizes the preparation and application of chromatographic separation media based on methacrylate monomers with a major focus on highly crosslinked macroporous beads prepared from 2-hydroxyethyl methacrylate and glycidyl methacrylate, respectively. The effects of process variables such as composition of the polymerization mixture that includes monomers, porogenic solvents, and free radical initiator, suspension stabilizer, reaction temperature, and stirring are detailed for both classical and templated suspension polymerization. In addition, specific features of the preparation of monodisperse beads are also discussed. The performance of methacrylate-based separation media is demonstrated on numerous separations in a variety of chromatographic modes.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Milan J
authors.initials: MJ
authors.lastname: Benes
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
authors.forename: Frantisek
authors.initials: F
authors.lastname: Svec
mesh_terms: D002845:Chromatography; D008689:Methacrylates; D008768:Methylmethacrylates; D008855:Microscopy, Electron, Scanning; D008863:Microspheres
chemical_list: D008689:Methacrylates; D008768:Methylmethacrylates; C020347:GMA-EDMA; C004919:ethylene dimethacrylate