title: Effect of orally administered non-viable Lactobacillus cells on murine humoral immune responses.
abstract: BALB/c mice were immunized intraperitoneally with the food antigen ovalbumin (OVA) while they were fed with Lactobacillus GG heated killed cell preparation. The oral administration of Lactobacillus GG did not appear to modify the antigen-augmented serum IgE in the tested mice but significantly augmented serum OVA specific IgG in the tested mice fed with a diet containing 0.1% Lactobacillus GG as the non-viable cell preparation (P< 0.05). The fecal OVA specific IgA of the tested mice fed with nonviable Lactobacillus GG cells was also significantly elevated (P< 0.05) compared to those from OVA immunized mice. The spleen cells of mice fed with non-viable Lactobacillus GG cells secreted more IL-6 (P< 0.01). These results suggest that the non-viable Lactobacillus GG can augment the systemic and mucosal immune responses in a host animal favoring secretory IgA but not IgE in an adjuvant-like manner.
authors.affiliation: Technical Research Laboratory, Takanashi Milk Products Co., Ltd, Yokohama, Kanagawa, Japan. he-fang@takanashi-milk.co.jp
authors.forename: Fang
authors.initials: F
authors.lastname: He
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Morita
authors.forename: Akira
authors.initials: A
authors.lastname: Kubota
authors.forename: Arthur C
authors.initials: AC
authors.lastname: Ouwehand
authors.forename: Masataka
authors.initials: M
authors.lastname: Hosoda
authors.forename: Masaru
authors.initials: M
authors.lastname: Hiramatsu
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kurisaki
mesh_terms: D000284:Administration, Oral; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D018928:Immunity, Mucosal; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007778:Lactobacillus / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D019936:Probiotics / Q000627:therapeutic use*; D013154:Spleen / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D010047:Ovalbumin