Quantitative analysis of Staphylococcus aureus in skimmed milk powder by real-time PCR.
A large-scale outbreak of food poisoning caused by consumption of skimmed milk powder contaminated with staphylococcal enterotoxin A (SEA) occurred in Japan. No viable Staphylococcus aureus was detected in the skimmed milk powder, however, sea and nuc genes of S. aureus were detected in it by PCR. The number of S. aureus in skimmed milk powder was estimated by quantitative real-time PCR.
Ikeda
Tetsuya
T
Hokkaido Institute of Public Health, Sapporo, Hokkaido, Japan.
Tamate
Naoto
N
Yamaguchi
Keiji
K
Makino
Sou-ichi
S
D000818:Animals; D001426:Bacterial Proteins; D017931:DNA Primers; D004720:Endonucleases; D004768:Enterotoxins / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay; D007564:Japan; D008836:Micrococcal Nuclease; D008892:Milk / Q000378:metabolism* / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D013202:Staphylococcal Food Poisoning / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D017931:DNA Primers; D004768:Enterotoxins; C074002:nuc protein, staphylococcus; C026066:enterotoxin A, Staphylococcal; D004720:Endonucleases; D008836:Micrococcal Nuclease