Effect of resistance exercise and carbohydrate ingestion on oxidative stress.
Some research studies have produced data indicating that resistance exercise induces oxidative stress, despite minimal increases in VO(2). These studies have primarily relied on oxidative stress markers with low sensitivity and debatable reliability. However, F(2)-isoprostanes as measured by gas chromatography mass spectrometry are considered to be a reliable and precise indicator of oxidative stress. Carbohydrate ingestion during exercise is associated with reduced levels of stress hormones, which may influence oxidative stress and plasma antioxidant potential. Therefore, the purpose of this study was to investigate the influence of carbohydrate ingestion during resistance training on F(2)-isoprostanes and plasma antioxidant potential. Thirty strength-trained subjects were randomized to carbohydrate (CHO) or placebo (PLA) groups that lifted weights for 2 h. Subjects received 10 ml kg(- 1) h(- 1) CHO (6%) or PLA beverages during the exercise. Blood and vastus lateralis muscle biopsy samples were collected before and after exercise and analyzed for cortisol as a marker of general stress, F(2)-isoprostanes as a measure of oxidative stress, and ferric reducing ability of plasma (FRAP) as a measure of antioxidant potential, and for muscle glycogen, respectively. Decreases in muscle glycogen content did not differ between CHO and PLA. Cortisol and FRAP increased significantly in CHO and PLA (P = 0.008 and 0.044, respectively), but the pattern of change was not different between groups. F(2)-isoprostanes were unaffected by exercise. These results indicate that exhaustive resistance exercise and carbohydrate ingestion have no effect on oxidative stress or plasma antioxidant potential in trained subjects.
McAnulty
Steven R
SR
Department of Health, Leisure and Exercise Science, Appalachian State University, Boone, NC 28608, USA. mcanltysr@appstate.edu
McAnulty
Lisa S
LS
Nieman
David C
DC
Morrow
Jason D
JD
Utter
Alan C
AC
Dumke
Charles L
CL
D000328:Adult; D000975:Antioxidants / Q000737:chemistry; D001706:Biopsy; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D002849:Chromatography, Gas; D015444:Exercise; D028441:F2-Isoprostanes / Q000737:chemistry; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D013058:Mass Spectrometry; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D009132:Muscles / Q000473:pathology; D018384:Oxidative Stress; D012680:Sensitivity and Specificity; D013314:Stress, Mechanical; D013997:Time Factors
D000975:Antioxidants; D002241:Carbohydrates; D028441:F2-Isoprostanes; D006003:Glycogen; D006854:Hydrocortisone