title: Decreased levels of CXC-chemokines in serum of benzene-exposed workers identified by array-based proteomics.
abstract: Benzene is an important industrial chemical and environmental contaminant that causes leukemia. To obtain mechanistic insight into benzene's mechanism of action, we examined the impact of benzene on the human serum proteome in a study of exposed healthy shoe-factory workers and unexposed controls. Two sequential studies were performed, each using sera from 10 workers exposed to benzene (overall mean benzene air level >30 ppm) and 10 controls. Serum samples were subjected to anion-exchange fractionation and bound to three types of ProteinChip arrays (Ciphergen Biosystems, Fremont, CA) [hydrophobic (H50), metal affinity (IMAC3-Cu), and cation exchange (WCX2)]. Protein-expression patterns were detected by surface-enhanced laser desorption/ionization (SELDI)-TOF MS. Three proteins (4.1, 7.7, and 9.3 kDa) were consistently down-regulated in exposed compared with control subjects in both studies. All proteins were highly inversely correlated with individual estimates of benzene exposure (r > 0.75). The 7.7- and 9.3-kDa proteins were subsequently identified as platelet factor (PF)4 and connective tissue activating peptide (CTAP)-III. Initial proteomic results for PF4 and CTAP-III were subsequently confirmed in a single experiment using a ProteinChip-array-based immunoassay(Ciphergen Biosystems). The altered expression of the platelet-derived CXC-chemokines (40% and 63% for PF4 and CTAP-III, respectively) could not be explained by changes in absolute platelet counts. Thus, SELDI-TOF analysis of a limited number of exposed and unexposed subjects revealed that lowered expression of PF4 and CTAP-III proteins is a potential biomarker of benzene's early biologic effects and may play a role in the immunosuppressive effects of benzene.
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, 6120 Executive Boulevard, Rockville, MD 20852, USA. vermeulr@mail.nih.gov
authors.forename: Roel
authors.initials: R
authors.lastname: Vermeulen
authors.forename: Qing
authors.initials: Q
authors.lastname: Lan
authors.forename: Luoping
authors.initials: L
authors.lastname: Zhang
authors.forename: Laura
authors.initials: L
authors.lastname: Gunn
authors.forename: Diane
authors.initials: D
authors.lastname: McCarthy
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Woodbury
authors.forename: Marielena
authors.initials: M
authors.lastname: McGuire
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Podust
authors.forename: Guilan
authors.initials: G
authors.lastname: Li
authors.forename: Nilanjan
authors.initials: N
authors.lastname: Chatterjee
authors.forename: Ruidong
authors.initials: R
authors.lastname: Mu
authors.forename: Songnian
authors.initials: S
authors.lastname: Yin
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Rothman
authors.forename: Martyn T
authors.initials: MT
authors.lastname: Smith
mesh_terms: D001554:Benzene / Q000009:adverse effects; D015415:Biomarkers / Q000097:blood; D019743:Chemokines, CXC / Q000097:blood*; D006801:Humans; D016014:Linear Models; D016273:Occupational Exposure / Q000009:adverse effects; D040081:Protein Array Analysis; D040901:Proteomics
chemical_list: D015415:Biomarkers; D019743:Chemokines, CXC; D001554:Benzene