2-Arachidonoylglycerol, an endogenous cannabinoid receptor ligand, enhances the adhesion of HL-60 cells differentiated into macrophage-like cells and human peripheral blood monocytes.
2-Arachidonoylglycerol (2-AG), an endogenous cannabionoid receptor (CB1 and CB2) ligand, enhanced the adhesion of HL-60 cells differentiated into macrophage-like cells to fibronectin and the vascular cell adhesion molecule-1. The CB2 receptor, Gi/Go, intracellular free Ca(2+) and phosphatidylinositol 3-kinase were shown to be involved in 2-AG-induced augmented cell adhesion. 2-AG also enhanced the adhesion of human monocytic leukemia U937 cells and peripheral blood monocytes. These results strongly suggest that 2-AG plays some essential role in inflammatory reactions and immune responses by inducing robust adhesion to extracellular matrix proteins and adhesion molecules in several types of inflammatory cells and immune-competent cells.
Gokoh
Maiko
M
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa 199-0195, Japan.
Kishimoto
Seishi
S
Oka
Saori
S
Metani
Yasuchika
Y
Sugiura
Takayuki
T
D001095:Arachidonic Acids / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects*; D002454:Cell Differentiation; D063388:Endocannabinoids; D005353:Fibronectins / Q000378:metabolism; D005989:Glycerides / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D008024:Ligands; D008264:Macrophages / Q000187:drug effects*; D009000:Monocytes / Q000187:drug effects; D037342:Pertussis Toxin / Q000494:pharmacology; D043885:Receptor, Cannabinoid, CB2 / Q000819:agonists* / Q000037:antagonists & inhibitors; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D001095:Arachidonic Acids; D063388:Endocannabinoids; D005353:Fibronectins; D005989:Glycerides; D008024:Ligands; D043885:Receptor, Cannabinoid, CB2; D019010:Vascular Cell Adhesion Molecule-1; C094503:glyceryl 2-arachidonate; D037342:Pertussis Toxin