title: Evaluation of nonstarch polysaccharides and oligosaccharide content of different soybean varieties (Glycine max) by near-infrared spectroscopy and proteomics.
abstract: A total of 832 samples of soybeans were screened by near-infrared (NIR) reflectance spectroscopy, to identify soybean samples with a lower content of oligosaccharides and nonstarch polysaccharides (NSP). Of these, 38 samples were identified on the basis of variation in protein content and agronomic value and submitted to high-resolution NIR spectroscopy. On the basis of the NIR data, 12 samples were further selected for chromatographic characterization of carbohydrate composition (mono-, di-, and oligosaccharides and NSP). Their soluble proteins were separated by two-dimensional gel electrophoresis (2DE). Using partial least-squares regression (PLSR), it was possible to predict the content of total NSP from the high-resolution NIR spectra, suggesting that NIR is a suitable and rapid nondestructive method to determine carbohydrate composition in soybeans. The 2DE analyses showed varying intensities of several proteins, including the glycinin G1 precursor. PLSR analysis showed a negative correlation between this protein and insoluble NSP and total uronic acid (UA).
authors.affiliation: Matforsk, The Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway. kristin.hollung@matforsk.no
authors.forename: Kristin
authors.initials: K
authors.lastname: Hollung
authors.forename: Margareth
authors.initials: M
authors.lastname: Øverland
authors.forename: Milica
authors.initials: M
authors.lastname: Hrustić
authors.forename: Petar
authors.initials: P
authors.lastname: Sekulić
authors.forename: Jegor
authors.initials: J
authors.lastname: Miladinović
authors.forename: Harald
authors.initials: H
authors.lastname: Martens
authors.forename: Bjørg
authors.initials: B
authors.lastname: Narum
authors.forename: Stefan
authors.initials: S
authors.lastname: Sahlstrøm
authors.forename: Mette
authors.initials: M
authors.lastname: Sørensen
authors.forename: Trond
authors.initials: T
authors.lastname: Storebakken
authors.forename: Anders
authors.initials: A
authors.lastname: Skrede
mesh_terms: D009844:Oligosaccharides / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*; D040901:Proteomics; D013025:Glycine max / Q000737:chemistry* / Q000145:classification*; D019265:Spectroscopy, Near-Infrared
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides