title: Antigenic specificity of the mucosal antibody response to Moraxella catarrhalis in chronic obstructive pulmonary disease.
abstract: Moraxella catarrhalis is an important human mucosal pathogen causing otitis media in children and lower respiratory tract infection in adults with chronic obstructive pulmonary disease (COPD). Little is known about the mucosal antibody response to M. catarrhalis in adults with COPD. In this study, 10 pairs of well-characterized sputum supernatant samples from adults with COPD who had acquired and subsequently cleared M. catarrhalis from their respiratory tracts were studied in detail in an effort to begin to elucidate potentially protective immune responses. Flow cytometry analysis was used to study the distribution of immunoglobulin isotypes in paired preacquisition and postclearance sputum samples. The results showed that immunoglobulin A (IgA) is the predominant M. catarrhalis-specific immunoglobulin isotype and that the sputum IgA contains a secretory component, indicating that it is locally produced at the mucosal site. Most patients made new sputum IgA responses to the adhesins UspA1 and Hag, along with the surface protein UspA2. A smaller proportion of patients made new sputum IgA responses to the iron-regulated proteins TbpB and CopB and to lipooligosaccharide. These results have important implications in understanding the mucosal immune response to M. catarrhalis in the setting of COPD and in elucidating the elements of a protective immune response.
authors.affiliation: VA Western New York Healthcare System, Medical Research 151, 3495 Bailey Avenue, Buffalo, NY 14215, USA. murphyt@buffalo.edu
authors.forename: Timothy F
authors.initials: TF
authors.lastname: Murphy
authors.forename: Aimee L
authors.initials: AL
authors.lastname: Brauer
authors.forename: Christoph
authors.initials: C
authors.lastname: Aebi
authors.forename: Sanjay
authors.initials: S
authors.lastname: Sethi
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis* / Q000096:biosynthesis; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis* / Q000096:biosynthesis; D007132:Immunoglobulin Isotypes / Q000032:analysis; D001936:Moraxella catarrhalis / Q000235:genetics / Q000276:immunology*; D045828:Moraxellaceae Infections / Q000276:immunology*; D009154:Mutation; D029424:Pulmonary Disease, Chronic Obstructive / Q000276:immunology* / Q000382:microbiology; D020545:Respiratory Mucosa / Q000276:immunology*; D013183:Sputum / Q000166:cytology / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C464644:Hag-1 protein, Moraxella catarrhalis; D007070:Immunoglobulin A; D007132:Immunoglobulin Isotypes; C089675:UspA protein, Moraxella catarrhalis