title: Evaluation of a rat model of experimental autoimmune encephalomyelitis with human MBP as antigen.
abstract: Experimental autoimmune encephalomyelitis (EAE) is a good model for human multiple sclerosis (MS) research. However, there are some defects in the traditional models. Here, we improved the model by using the human myelin basic protein (MBP) as antigen. EAE was induced by immunization of female Wistar rats with human MBP. Compared with the traditional models, the new model was evaluated by clinical signs to pathological changes. The immune state of the model was assessed by the lymphocyte infiltrative response and levels of TNF-alpha, IFN-gamma, IL-10. It was found that most of rats exhibited tail tone loss and hind-limb paralysis, also there were demyelination, infiltrative lymphocyte foci, "neuronophagia" in the cortex of cerebra and the white matter of spinal cords. PBMCs and spleen lymphocytes were strongly responsive to the stimulation of MBP and PHA. The levels of TNF-alpha and IFN-gamma were altered with the severity of EAE. In the remitting phase, IL-10 was increased significantly. This study demonstrates that the animal model of EAE induced by human MBP bears resemblance to the features of human multiple sclerosis and promises to be a better model than ever before for the study of MS.
authors.affiliation: Department of Pathophysiology, Nanjing Medical University, Nanjing 210029, China. guolin0724@163.com
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Li
authors.forename: Hongyi
authors.initials: H
authors.lastname: Lin
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ji
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Lingli
authors.initials: L
authors.lastname: Que
authors.forename: Yingdong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yushan
authors.initials: Y
authors.lastname: Rong
authors.forename: Jianwen
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000097:blood* / Q000473:pathology; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D009103:Multiple Sclerosis / Q000097:blood* / Q000473:pathology; D004676:Myelin Basic Protein / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016207:Cytokines; D004676:Myelin Basic Protein