title: Synthesis of oxytocin analogues with replacement of sulfur by carbon gives potent antagonists with increased stability.
abstract: [Chemical reaction: See text] The neuropeptide oxytocin 1 controls mammary and uterine smooth muscle contraction. Atosiban 2, an oxytocin antagonist, is used for prevention of preterm labor and premature birth. However, the metabolic lifetimes of such peptide drugs are short because of in vivo degradation. Facile production of oxytocin analogues with varying ring sizes wherein sulfur is replaced by carbon (methylene or methine) could be achieved by standard solid-phase peptide synthesis using olefin-bearing amino acids followed by on-resin ring-closing metathesis (RCM). These were tested for agonistic and antagonistic uteronic activity using myometrial strips taken from nonpregnant female rats. Peptide 8 showed agonistic activity in vitro (EC50= 1.4 x 10(3) +/- 4.4 x 10(2) nM) as compared to 1 (EC50= 7.0 +/- 2.1 nM). Atosiban analogues 17 (pA2= 7.8 +/- 0.1) and 18 (pA2= 8.0 +/- 0.1) showed substantial activity compared to the parent oxytocin antagonist 2 (pA2= 9.9 +/- 0.3). Carba analogue 35 (pA2= 6.1 +/- 0.1) had an agonistic activity over 2 orders of magnitude less than its parent 3 (8.8 +/- 0.5). A comparison of biological stabilities of 1,6-carba analogues of both an agonist 8 and antagonist 18 versus parent peptides 1 and 2 was conducted. The half-lives of peptides 8 and 18 in rat placental tissue were shown (Table 2) to be greatly improved versus their parents oxytocin 1 and atosiban 2, respectively. These results suggest that peptides 8 and 18 and analogues thereof may be important leads into the development of a long-lasting, commercially available therapeutic for initiation of parturition and treatment of preterm labor.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Jake L
authors.initials: JL
authors.lastname: Stymiest
authors.forename: Bryan F
authors.initials: BF
authors.lastname: Mitchell
authors.forename: Susan
authors.initials: S
authors.lastname: Wong
authors.forename: John C
authors.initials: JC
authors.lastname: Vederas
mesh_terms: D002244:Carbon; D005260:Female; D006801:Humans; D008958:Models, Molecular; D008968:Molecular Conformation; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000138:chemical synthesis*; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry; D013455:Sulfur; D015149:Tocolytic Agents / Q000138:chemical synthesis; D014599:Uterus / Q000187:drug effects / Q000502:physiology; D014668:Vasotocin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D010456:Peptides, Cyclic; D015149:Tocolytic Agents; C047046:atosiban; D010121:Oxytocin; D013455:Sulfur; D002244:Carbon; D014668:Vasotocin