[Level of nitric oxide and tumor necrosis factor-alpha production by human blood neutrophils under UV-irradiation].
The effect of the UV-irradiation (75.5-2265 J/m2) on the nitrogen oxide (NO) and the tumor necrosis factor-alpha (TNF-alpha) production by neutrophils of human blood was studied by Griess method based on the accumulation of NO2(-) -ions in the incubation matter and on the enzyme-linked immunosorbent assay (ELISA). UV-irradiation in the dose of 75.5 J/m2 reveals the correctional effect relatively to the native level of NO, providing the maintenance of the physiological homeostasis. The irradiation of the neutrophils by the doses of 755 and 2265 J/m2 increases the concentration of NO metabolites, that might lead to different pathologies. The process of NO-synthesis stopped during the incubation of these cells in the presence of the transcriptional inhibitor of the protein sunthesis - cycloheximid. This suggests that the NO generation by photomodified neutrophils is due to the activation of iNOS synthesis de novo by UV-irradiation. UV-irradiation also has a modulating effect on the level of TNF-alpha production by phagocytes. The resulting effect depends on the native cytokine concentration. The positive correlation between NO and TNF-alpha concentrations in native and irradiated cells in the dose of 755 J/m2 was estimated, that shows close interrelationship between these parameters.
Artiukhov
V F
VF
Gusinskaia
V V
VV
Mikhileva
E A
EA
D004307:Dose-Response Relationship, Radiation; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D014466:Ultraviolet Rays
D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II