Identification of a new HLA-B allele, HLA-B*4443, in a German family.
A novel human leukocyte antigen (HLA)-B allele is described. The allele was identified in a German blood donor of Caucasian origin. Because high-resolution HLA-typing using sequence-specific primers gave inconclusive results, sequence-based typing was performed. Nucleotide sequences of exons 2 and 3 most closely match with HLA-B*4417 and HLA-B*440301 (99.5% identity). The predicted protein sequence revealed a single amino acid substitution (D156L) compared with the HLA-B*4417 allele but two substitutions (Y113H, D116S) compared with the HLA-B*440301 allele. Therefore, the novel allele has been officially assigned HLA-B*4443 by the WHO Nomenclature Committee. The HLA-B*4443 allele was found with the A*2301, Cw*0401, B*4443, DRB1*0701, DRB4*0107, and DQB1*0202 haplotype.
Thude
H
H
Institute for Transfusion Medicine, University Hospital of Jena, Germany. hansjoerg.thude@med.uni-jena.de
Hardt
C
C
Schorner
U
U
Ferencik
S
S
Helfricht
C
C
Barz
D
D
D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D005091:Exons / Q000235:genetics*; D005260:Female; D005858:Germany; D015235:HLA-B Antigens / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D044465:White People
D015235:HLA-B Antigens