title: Polymorphisms of the prion protein gene (PRNP) in Hanwoo (Bos taurus coreanae) and Holstein cattle.
abstract: Polymorphisms in the prion protein gene (PRNP) in humans and sheep correlate with susceptibility to transmissible spongiform encephalopathies (TSEs). Bovine spongiform encephalopathy (BSE) has been reported in British and Japanese cattle; it has occurred thus far in Holstein cattle. BSE in Hanwoo (Bos taurus coreanae) cattle has not been diagnosed up to now. To characterize the bovine PRNP polymorphisms in Korean cattle, we analyzed the open reading frame (ORF) of PRNP in 120 Hanwoo (beef) cattle and 53 Holstein (dairy) cattle. Three polymorphisms were found, the third position of codon 78 (G-->A), the third position of codon 192 (C-->T), and the deletion of a single octa-repeat. An analysis of codon 78 revealed no difference in the genotype (P = 0.2026) or allele (P = 0.7180) frequencies between Hanwoo and Holstein animals. However, there were significant differences in the genotype (P < 0.0001) and allele (P < 0.0001) frequencies at PRNP codon 192 between Hanwoo and Holstein animals. The rate of Holstein animals with deletion of a single octa-repeat was 91.5% undeleted homozygotes, 8.5% heterozygotes (with R3 deletion), and 0% deleted homozygotes. However, none of the 120 Hanwoo animals had any octa-repeat deletions. The genotype (P < 0.0001) and allele (P < 0.0001) frequencies of a single octa-repeat-deletion were also significantly different between Hanwoo and Holstein animals.
authors.affiliation: Ilsong Institute of Life Science, Hallym University, Anyang, Kyounggi-do, South Korea.
authors.forename: Byung-Hoon
authors.initials: BH
authors.lastname: Jeong
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Sohn
authors.forename: Jong-O
authors.initials: JO
authors.lastname: Lee
authors.forename: Nam-Ho
authors.initials: NH
authors.lastname: Kim
authors.forename: Jae-Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Cho
authors.forename: Yi-Seok
authors.initials: YS
authors.lastname: Joo
authors.forename: Richard I
authors.initials: RI
authors.lastname: Carp
authors.forename: Yong-Sun
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000483:Alleles; D000682:Amyloid / Q000235:genetics*; D000818:Animals; D002417:Cattle; D003062:Codon / Q000235:genetics; D016643:Encephalopathy, Bovine Spongiform; D005787:Gene Frequency; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D016366:Open Reading Frames / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D000072002:Prion Proteins; D011328:Prions; D011498:Protein Precursors / Q000235:genetics*; D017384:Sequence Deletion; D012756:Sheep
chemical_list: D000682:Amyloid; D003062:Codon; C512656:PRNP protein, human; D000072002:Prion Proteins; D011328:Prions; D011498:Protein Precursors