Efficient solid-phase synthesis of trifunctional probes and their application to the detection of carbohydrate-binding proteins.
[structure: see text] An efficient solid-phase synthesis of trifunctional probes containing a photoreactive group, a reporter tag, and a carbohydrate ligand was developed. Labeling studies with these probes demonstrate that specific lectins can be labeled with high sensitivity and selectivity. This technique serves as a powerful tool for the rapid detection and profiling of lectins.
Lee
Myung-ryul
MR
Department of Chemistry, Yonsei University, Seoul 120-749, Korea.
Jung
Da-Woon
DW
Williams
Darren
D
Shin
Injae
I
D002241:Carbohydrates / Q000737:chemistry*; D037102:Lectins / Q000032:analysis* / Q000737:chemistry; D008024:Ligands; D015335:Molecular Probes / Q000138:chemical synthesis*; D015394:Molecular Structure
D002241:Carbohydrates; D037102:Lectins; D008024:Ligands; D015335:Molecular Probes