title: Evaluation of supercritical fluid extraction/aminopropyl solid-phase "in-line" cleanup for analysis of pesticide residues in rice.
abstract: Supercritical fluid extraction (SFE) and the use of aminopropyl solid-phase material for "in-line" cleanup was evaluated for residue analysis of 22 GC-amenable pesticides in wild- and white-rice samples with a fat content of 1.9 and 0.4%, respectively. After optimizing the extraction conditions on glass beads as inert material and evaluating the fat amount extracted from rice by SFE, the use of Florisil, Celite, Extrelut, Hydromatrix, and an aminopropyl material as fat-retention materials for SFE "in-line" cleanup was assessed, aminopropyl being the most suitable material for this cleanup of fat. Pesticide mean recoveries obtained from rice samples, at fortification levels around 0.5 mg/kg, by means of the SFE/in-line cleanup method finally proposed (15-mL CO2 volume, 50 degrees C temperature, 200 atm pressure, 200 muL of methanol static modifier, and a 1-cm layer of aminopropyl at the bottom of the extraction vessel), ranged between 74 and 98%, except for captafol and dimethoate for which mean recoveries lower than 21% were determined.
authors.affiliation: Pesticide Residue Research Group, Faculty of Experimental Sciences, University of Almería, 04071 Almería, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Aguilera
authors.forename: Mariano
authors.initials: M
authors.lastname: Rodríguez
authors.forename: Maria
authors.initials: M
authors.lastname: Brotons
authors.forename: Mourad
authors.initials: M
authors.lastname: Boulaid
authors.forename: Antonio
authors.initials: A
authors.lastname: Valverde
mesh_terms: D025924:Chromatography, Supercritical Fluid; D005898:Glass; D008863:Microspheres; D012275:Oryza / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D011437:Propylamines
chemical_list: D010573:Pesticide Residues; D011437:Propylamines