[The product identification study on the isolating process of strains produced trehalose].
In the isolating process of stains whose endocellular enzymes can produce trehalose on starch or maltooligosaccharides, we discovered the components of enzymatic reactant were complicated and it was difficult to purify them each other, however, we have to know quickly whether there was tehalose in the enzymatic reactant above. In order to make it clearly, thinner layer chromatography, high performance liquid chromatography-electrospray ionization mass spectrometry and 13C-nuclear magnetic resonance were adopted. The unknown oligosaccharide produced by Arthrobacter nicotinovorus D-97 was identified rapidly and this unknown oligosaccharide needn't be purified from the enzymatic reactant solely. The production on starch or maltooligosaccharides by endocellular enzymes of Arthrobacter nicotinovorus D-97 is trehalose. This research method in our paper can be applied in the isolating process of production trehalose and other functional glucobioses strains.
Rong
Shaofeng
S
The School of Biotechnology, Southern Yangzte University, Wuxi 214036, China. rongshaofeng@yahoo.com
Zhang
Haiping
H
Yang
Jing
J
Duan
Zuoying
Z
Mao
Zhonggui
Z
D001173:Arthrobacter / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D009682:Magnetic Resonance Spectroscopy; D021241:Spectrometry, Mass, Electrospray Ionization; D014199:Trehalose / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry
D014199:Trehalose