[Research of the hemostasis effect of chitosan acetic acid solution].
The effect of chitosan with different molecular weight and deacetylation degree on blood hemostasis was tested. The experiments found evident alteration of red blood cell morphology and unusual coagglutination between erythrocytes in the anticoagulant blood which was treated by chitosan acetic acid solution. The red blood cells clot formation experiments showed that chitosan with low deacetylation degree (60%-70%) caused more red blood cells to assemble when compared versus chitosan with other deacetylation degrees. The effect of molecular weight between 10(5)-10(6) was not obvious. The thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), and concentration of fibrinogen (FIB) of blood treated by chitosan acetic acid solution were measured. The results proved that the hemostasis property of chitosan acetic acid solution was independent of the platelets and the normal "Cascade-like" coagulation pathway.
Tian
Feng
F
The Institute of Medical Equipment, Academy of Military Medical Science, Tianjin 300161, China. tianfeng56754@eyon.com
Yang
Jian
J
Chen
Shiqian
S
Kan
Yaodong
Y
Ji
Fengxiang
F
D019342:Acetic Acid / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D005340:Fibrinogen / Q000032:analysis; D006490:Hemostatics / Q000737:chemistry / Q000494:pharmacology*; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D011817:Rabbits; D013918:Thrombin Time
D006490:Hemostatics; D005340:Fibrinogen; D048271:Chitosan; D019342:Acetic Acid