[Characterization of Amycolatopsis meditteranei U32 chromosome by pulse-field gel electrophoresis].
Amycolatopsis meditteranei U32 is an industrial strain with high yield of rifamycin SV. Its chromosome structure was characterized by pulse-field gel electrophoresis. The topological structure of U32 chromosome was linear and its genome size was about 10 Mb. There was no internal plasmid found. Gene encoding several enzymes involved in rifamycin bio-synthesis and relevant primary metabolism as well as protein related to metabolic regulation were localized by Southern blot. The results revealed that there were at least five copies of rrn operon in U32. The rifamycin bio-synthesis gene cluster was located on one about 700 kb PshBI fragement where several other important genes related to primary and secondary metabolism were also situated. They were the nasD gene, the mcm gene encoding methylmalonyl CoA mutase, the accA gene encoding acethyl CoA carboxylase biotin carrier protein and one copy of the rrn operons.
Ma
Wei
W
The Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. wma@sjtu.edu.cn
Yao
Yufeng
Y
Jiang
Weihong
W
Jiao
Ruishen
R
Zhao
Guoping
G
D000192:Actinomycetales / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D008969:Molecular Sequence Data