Rapid and sensitive liquid chromatography-mass spectrometry method for determination of ropinirole in human plasma.
A rapid and robust liquid chromatography-mass spectrometry (LC-MS/MS) method was developed for non-ergoline dopamine D(2)-receptor agonist, ropinirole in human plasma using Es-citalopram oxalate as an internal standard. The method involves solid phase extraction from plasma, reversed-phase simple isocratic chromatographic conditions and mass spectrometric detection that enables a detection limit at picogram levels. The proposed method was validated with linear range of 20-1,200 pg/ml. The extraction recoveries for ropinirole and internal standard were 90.45 and 65.42%, respectively. The R.S.D.% of intra-day and inter-day assay was lower than 15%. For its sensitivity and reliability, the proposed method is particularly suitable for pharmacokinetic studies.
Bhatt
Jignesh
J
Torrent Research Centre, Gandhinagar 380009, Gujarat, India. jigu_baroda@rediffmail.com
Jangid
Arvind
A
Shetty
Raghavendra
R
Shah
Bhavin
B
Kambli
Sandeep
S
Subbaiah
Gunta
G
Singh
Sadhana
S
D002138:Calibration; D002853:Chromatography, Liquid; D015283:Citalopram / Q000097:blood / Q000737:chemistry; D018491:Dopamine Agonists / Q000097:blood* / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents; D007211:Indoles / Q000097:blood* / Q000737:chemistry; D013058:Mass Spectrometry; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D017367:Selective Serotonin Reuptake Inhibitors / Q000097:blood / Q000737:chemistry; D012996:Solutions
D015283:Citalopram; D018491:Dopamine Agonists; D007202:Indicators and Reagents; D007211:Indoles; D017367:Selective Serotonin Reuptake Inhibitors; D012996:Solutions; C046649:ropinirole