The heme-binding lipoprotein (HbpA) of Haemophilus influenzae: role in heme utilization.
Haemophilus influenzae has an absolute growth requirement for heme and a heme binding lipoprotein (HbpA) has been implicated in the utilization of this essential nutrient. HbpA was identified by examining clones from an H. influenzae genomic library that caused Escherichia coli harboring the clone to bind heme. However, HbpA has not been shown to mediate heme acquisition in H. influenzae. We constructed an insertional mutation of hbpA in a nontypeable H. influenzae strain and demonstrated a role for the gene in utilization of multiple heme sources. This is the first report confirming a role for HbpA in utilization of heme.
Morton
Daniel J
DJ
Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Madore
Larissa L
LL
Smith
Ann
A
Vanwagoner
Timothy M
TM
Seale
Thomas W
TW
Whitby
Paul W
PW
Stull
Terrence L
TL
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D015321:Gene Rearrangement; D006193:Haemophilus influenzae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D006418:Heme / Q000378:metabolism*; D008074:Lipoproteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation
D001426:Bacterial Proteins; D002352:Carrier Proteins; C097952:HbpA protein, Haemophilus influenzae; D008074:Lipoproteins; D006418:Heme