Enumerating leptospires using the coulter counter.
The currently accepted gold standard for enumeration of leptospires is tabulation of organisms using a Petroff-Hausser counting chamber and dark-field microscopy, a technically demanding, time-consuming technique. Quantification of leptospires with a Coulter counter produced reliable and reproducible counts, comparable to the counting chamber. This experiment demonstrates that the faster, less technically demanding Coulter counter may be an alternative to determine numbers of leptospires.
Humberd
Christina M
CM
Infectious Diseases Department, Wilford Hall USAF Medical Center, San Antonio, Texas 78236, USA. Christina.Humberd@lackland.af.mil
Murray
Clinton K
CK
Stuart
Saundra K
SK
Reeb
Barbara A
BA
Hospenthal
Duane R
DR
D001431:Bacteriological Techniques; D015169:Colony Count, Microbial / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007919:Leptospira / Q000145:classification / Q000302:isolation & purification; D008853:Microscopy / Q000379:methods; D012703:Serotyping