A modified automated high-throughput mycobacterial interspersed repetitive unit method for genotyping Mycobacterium tuberculosis.
A total of 502 isolates of Mycobacterium tuberculosis collected in Taiwan were genotyped using a modified and high-throughput typing system involving mycobacterial interspersed repetitive units. The Hunter-Gaston discriminatory index improved significantly to 0.972 with the modified 15-locus scheme, including ETR-A, ETR-B, and ETR-C, compared with 0.951 for the previously reported 12-locus design.
Chin
Pei-Ju
PJ
Reference Laboratory of Mycobacteriology, Center for Disease Control, Department of Health, Taipei, Taiwan 115, ROC.
Jou
Ruwen
R
D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D020071:Interspersed Repetitive Sequences / Q000235:genetics*; D018598:Minisatellite Repeats; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013624:Taiwan; D014376:Tuberculosis / Q000382:microbiology
D004269:DNA, Bacterial