title: Corneal epithelial homeostasis following daily and overnight contact lens wear.
abstract: This report reviews and integrates our recent clinical and laboratory findings of the effects of daily (DW)/overnight (EW) contact lens wear on corneal epithelial homeostasis. In a prospective, double-masked human clinical study, three lens types (high and hyper Dk/t soft; hyper Dk/t RGP) were used to assess the effects of daily and overnight lens wear on corneal epithelial thickness, cell surface size, Pseudomonas aeruginosa (PA) binding to exfoliated epithelial cells and exfoliation rates. Concomitantly, we studied in a rabbit model, the effects of overnight lens wear on corneal epithelial surface cell viability (live/dead assay, TUNEL-labeling, Annexin-V staining, Bcl-2 expression), basal cell proliferation and migration patterns. The comprehensive results obtained yield important new insights on both the normal homeostasis of the corneal epithelium and the biological changes induced by contact lens wear.
authors.affiliation: Department of Ophthalmology, Southwestern Medical Center, University of Texas, 5323 Harry Hines Boulevard, Dallas, TX 75390-9057, USA.
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Ladage
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: David H
authors.initials: DH
authors.lastname: Ren
authors.forename: W Matthew
authors.initials: WM
authors.lastname: Petroll
authors.forename: James V
authors.initials: JV
authors.lastname: Jester
authors.forename: H Dwight
authors.initials: HD
authors.lastname: Cavanagh