Application of a static fluorescence-based cytometer (the CellScan) in basic cytometric studies, clinical pharmacology, oncology and clinical immunology.
The CellScan apparatus is a laser scanning cytometer enabling repetitive fluorescence intensity (FI) and polarization (FP) measurements in living cells, as a means of monitoring lymphocyte activation. The CellScan may serve as a tool for diagnosis of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) as well as other autoimmune diseases by monitoring FP changes in peripheral blood lymphocytes (PBLs) following exposure to autoantigenic stimuli. Changes in FI and FP in atherosclerotic patients' PBLs following exposure to various stimuli have established the role of the immune system in atherosclerotic disease. The CellScan has been evaluated as a diagnostic tool for drug-allergy, based on FP reduction in PBLs following incubation with allergenic drugs. FI and FP changes in cancer cells have been found to be well correlated with the cytotoxic effect of anti-neoplastic drugs. In conclusion, the CellScan has a variety of applications in cell biology, immunology, cancer research and clinical pharmacology.
Harel
Michal
M
Center for Autoimmune Diseases, Department of Medicine B, Chaim Sheba Medical Center, Israel.
Gilburd
Boris
B
Schiffenbauer
Yael S
YS
Shoenfeld
Yehuda
Y
D000486:Allergy and Immunology / Q000295:instrumentation*; D017209:Apoptosis; D015551:Autoimmunity; D003592:Cytophotometry / Q000295:instrumentation*; D004342:Drug Hypersensitivity / Q000175:diagnosis; D004798:Enzymes / Q000378:metabolism; D005434:Flow Cytometry; D005454:Fluorescence Polarization / Q000295:instrumentation*; D006801:Humans; D019044:Image Cytometry; D008213:Lymphocyte Activation; D008495:Medical Oncology / Q000295:instrumentation; D009369:Neoplasms / Q000175:diagnosis / Q000276:immunology; D010601:Pharmacology, Clinical / Q000295:instrumentation
D004798:Enzymes