[Preparation of Pseudomonas aeruginosa recombinant outer-membrane protein F and the study of its antigenic properties].
In Escherichia coli M15, the gene of P. aeruginosa recombinant outer-membrane protein F (OprF) was cloned. OprF, chromatographically purified on Ni-agarose and containing an additional sequence of 6 histidines on the N-end, was obtained. The purified OprF specifically reacted with rabbit serum, hyperimmune to P. aeruginosa, and in the mice injected with this protein specific IgG antibodies were synthesized. The optimum concentrations of P. aeruginosa OprF were selected for further tests of its protective properties from infection induced by P. aeruginosa.
Kaloshin
A A
AA
Zlygostev
S A
SA
Toropchina
Iu N
IuN
Kurbatova
E A
EA
Zverev
V V
VV
Mikhaĭlova
N A
NA
D018829:Adhesins, Bacterial / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D002845:Chromatography; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D006639:Histidine; D007106:Immune Sera / Q000032:analysis; D007114:Immunization; D007115:Immunization Schedule; D007074:Immunoglobulin G / Q000097:blood*; D051379:Mice; D018272:Porins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D011552:Pseudomonas Infections / Q000097:blood / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera; D007074:Immunoglobulin G; D018272:Porins; D011994:Recombinant Proteins; D006639:Histidine