title: Influence of beta(2)-adrenoceptor gene polymorphisms on diet-induced thermogenesis.
abstract: The sympathetic nervous system is involved in the control of energy metabolism and expenditure. Diet-induced thermogenesis is mediated partly by the ss-adrenergic component of this system. The aim of the present study was to investigate the role of genetic variation in the beta(2)-adrenoceptor in diet-induced thermogenesis. Data from twenty-four subjects (fourteen men and ten women; BMI 26.7(sem 0.8) kg/m(2); age 45.2(sem1.4) years) with different polymorphisms of the beta(2)-adrenoceptor at codon 16 (Gly16Gly, Gly16Arg or Arg16Arg) were recruited for this study. Subjects were given a high-carbohydrate liquid meal, and the energy expenditure, respiratory exchange ratio, and plasma concentrations of NEFA, glycerol, glucose, insulin and catecholamines were measured before and over 4 h after the meal. The AUC of energy expenditure (diet-induced thermogenesis) was not significantly different between polymorphism groups, nor was the response of any of the other measured variables to the meal. In a multiple regression model, the only variable that explained a significant proportion (32 %) of the variation in diet-induced thermogenesis was the increase in plasma adrenaline in response to the meal (P<0.05). The beta(2)-adrenoceptor codon16 polymorphisms did not contribute significantly. In conclusion, an independent contribution of the codon 16 polymorphism of the beta(2)-adrenoceptor gene to the variation in thermogenic response to a high-carbohydrate meal could not be demonstrated. The interindividual variation in thermogenic response to the meal was correlated with variations in the plasma adrenaline response to the meal.
authors.affiliation: Department of Human Biology/NUTRIM, Maastricht University, Maastricht, The Netherlands. j.oomen@hb.unimaas.nl
authors.forename: J M
authors.initials: JM
authors.lastname: Oomen
authors.forename: P M C M
authors.initials: PM
authors.lastname: Waijers
authors.forename: C
authors.initials: C
authors.lastname: van Rossum
authors.forename: B
authors.initials: B
authors.lastname: Hoebee
authors.forename: W H M
authors.initials: WH
authors.lastname: Saris
authors.forename: M A
authors.initials: MA
authors.lastname: van Baak
mesh_terms: D000273:Adipose Tissue / Q000502:physiology; D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D002395:Catecholamines / Q000097:blood; D004032:Diet; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005990:Glycerol / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000502:physiology*; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics*; D012119:Respiration; D022722:Thermogenesis / Q000502:physiology*
chemical_list: D001786:Blood Glucose; D002395:Catecholamines; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D018343:Receptors, Adrenergic, beta-2; D005990:Glycerol