title: Sulfite facilitates LDL lipid oxidation by transition metal ions: a pro-oxidant in wine?
abstract: Lipid oxidation in LDL may play a role in atherogenesis. It has been shown that sulfite - a compound in the aqueous fraction of wine - could inhibit free radical (AAPH) mediated oxidation of plasma. Thus, sulfite has been proposed as an antioxidant. In contrast, the aqueous phase of wine has recently been shown to contain not fully identified compounds promoting transition metal ion (Cu(2+)) initiated LDL oxidation. As transition metal ions can catalyse the auto-oxidation of sulfite, we studied the influence of sulfite on Cu(2+) initiated LDL oxidation. The results show that sulfite at concentrations found in vivo strongly facilitated LDL oxidation by Cu(2+). The LDL-oxidase activity of ceruloplasmin was also stimulated by sulfite. ROS formation by Cu(2+)/SO(3)(2-) was not inhibited by SOD but by catalase. We propose that formation of Cu(+), sulfite radicals (SO(3)*(-)) and hydroxyl radicals (OH(*)) is a mechanism by which sulfite could act as a pro-atherogenic agent in presence of transition metal ions.
authors.affiliation: Centre of Physiology and Pathophysiology, Department of Medical Chemistry, Medical University Vienna.
authors.forename: Hildegard
authors.initials: H
authors.lastname: Laggner
authors.forename: Marcela
authors.initials: M
authors.lastname: Hermann
authors.forename: Brigitte
authors.initials: B
authors.lastname: Sturm
authors.forename: Bernhard M K
authors.initials: BM
authors.lastname: Gmeiner
authors.forename: Stylianos
authors.initials: S
authors.lastname: Kapiotis
mesh_terms: D000578:Amidines / Q000737:chemistry; D002413:Cations, Divalent / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D008077:Lipoproteins, LDL / Q000737:chemistry*; D016877:Oxidants / Q000737:chemistry*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000737:chemistry; D013447:Sulfites / Q000737:chemistry*; D028561:Transition Elements / Q000737:chemistry; D014920:Wine
chemical_list: D000578:Amidines; D002413:Cations, Divalent; D008077:Lipoproteins, LDL; D016877:Oxidants; D017382:Reactive Oxygen Species; D013447:Sulfites; D028561:Transition Elements; C046728:2,2'-azobis(2-amidinopropane); D003300:Copper