title: Polyomavirus EGFP-pseudocapsids: analysis of model particles for introduction of proteins and peptides into mammalian cells.
abstract: A vector for preparation of mouse polyomavirus capsid-like particles for transfer of foreign peptides or proteins into cells was constructed. Model pseudocapsids carrying EGFP fused with the C-terminal part of the VP3 minor protein (EGFP-VLPs) have been prepared and analysed for their ability to be internalised and processed by mouse cells and to activate mouse and human dendritic cells (DC) in vitro. EGFP-VLPs entered mouse epithelial cells, fibroblasts and human and mouse DC efficiently and were processed by both, lysosomes and proteasomes. Surprisingly, they did not induce upregulation of DC co-stimulation molecules or maturation markers in vitro; however, they did induce interleukin 12 secretion.
authors.affiliation: Genetics and Microbiology, Faculty of Science, Charles University in Prague, Vinicná 5, 128 44 Prague 2, Czech Republic.
authors.forename: E
authors.initials: E
authors.lastname: Boura
authors.forename: D
authors.initials: D
authors.lastname: Liebl
authors.forename: R
authors.initials: R
authors.lastname: Spísek
authors.forename: J
authors.initials: J
authors.lastname: Fric
authors.forename: M
authors.initials: M
authors.lastname: Marek
authors.forename: J
authors.initials: J
authors.lastname: Stokrová
authors.forename: V
authors.initials: V
authors.lastname: Holán
authors.forename: J
authors.initials: J
authors.lastname: Forstová
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D003713:Dendritic Cells / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D018664:Interleukin-12 / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D010455:Peptides / Q000235:genetics*; D011120:Polyomavirus / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D014161:Transduction, Genetic / Q000379:methods*; D014771:Virion / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D010455:Peptides; D011506:Proteins; D049452:Green Fluorescent Proteins; D018664:Interleukin-12