Characterization of a human SWI2/SNF2 like protein hINO80: demonstration of catalytic and DNA binding activity.
The proteins belonging to SWI2/SNF2 family of DNA dependent ATPases are important members of the chromatin remodeling complexes that are implicated in epigenetic control of gene expression. We have identified a human gene with a putative DNA binding domain, which belongs to the INO80 subfamily of SWI2/SNF2 proteins. Here we report the cloning, expression, and functional activity of the domains from hINO80 gene both in terms of the DNA dependent ATPase as well as DNA binding activity. A differential expression of the various domains within this gene is detected in human tissues while a ubiquitous expression is detected in mice. The intranuclear localization is demonstrated using antibodies directed against the DBINO domain of hINO80.
Bakshi
Rachit
R
Dr. B. R. Ambedkar Centre for Biomedical Research, University of Delhi, Delhi-110007, India.
Mehta
Abhishek Kumar
AK
Sharma
Ritu
R
Maiti
Souvik
S
Pasha
Santosh
S
Brahmachari
Vani
V
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D009928:Organ Specificity; D017434:Protein Structure, Tertiary
D004268:DNA-Binding Proteins; D000251:Adenosine Triphosphatases; D004265:DNA Helicases