[Chemical modification of Streptococcus suis type 2 haemolysin].
The haemolysin of Streptococcus suis type 2 Jiangsu isolate was purified. Effects of protein modification reagents on the haemolysin activity were detected. The haemolysin was not affected by PCMB, succinic anhydride (SA), EDC, and N-AI modification, and it indicated that sulfhudryl groups, amino groups, carboxyl groups and tyrosine residues were not essential to haemolysin activity. The haemolysin activity was significantly decreased after NBS, DEPC, 2, 3-Diacetyl, H2O2 modification and was greatly increased after DTT modification. The results indicated that tryptophane residues, histidine residues, arginine residues and disulfides groups seemed to be essential to the haemolysin activity.
Fang
Shaoqing
S
Key Lab Animal Disease Diagnostic & Immunology, Ministry of Agricultural, Nanjing Agricultural University, Nanjing 210095, China.
Lu
Chengping
C
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000378:metabolism; D009930:Organic Chemicals / Q000737:chemistry; D055550:Protein Stability; D017011:Streptococcus suis / Q000737:chemistry* / Q000378:metabolism
D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D009930:Organic Chemicals