Association between anti-nucleophosmin and anti-cardiolipin antibodies in (NZW x BXSB)F1 mice and human systemic lupus erythematosus.
We showed previously that nucleophosmin (NPM), a nucleolar phosphoprotein, is recognized by sera from (NZW x BXSB)F1 (WB) mice, a model of systemic lupus erythematosus (SLE) and anti-phospholipid syndrome. In the present study we analysed the prevalence and kinetics of anti-NPM autoantibodies in WB mice by a solid-phase ELISA with recombinant human (rh) NPM as the antigen and showed that most male WB mouse sera had anti-NPM antibodies that were responsible for their indirect immunofluorescence staining pattern on Hep-2 cells. Anti-NPM antibodies were significantly associated with anti-cardiolipin (aCL) antibodies. This antibody profile mirrored that observed in certain human SLE sera because anti-NPM antibodies were detected in 28% of the sera from patients with SLE and were similarly associated with aCL antibodies. The demonstration that rhNPM bound to cardiolipin (CL) in vitro and increased the CL-binding activity of a WB-derived aCL monoclonal antibody indicates that NPM can interact with CL to form SLE-related immunogenic particles that might be responsible for the concomitant production of anti-NPM and aCL antibodies.
Lartigue
Aurelia
A
INSERM U519 and Institut Fédératif de Recherche Multidisciplinaire sur les Peptides, Faculté de Médecine et Pharmacie, 22 boulevard Gambetta, 76183 Rouen Cedex, France.
Drouot
Laurent
L
Jouen
Fabienne
F
Charlionet
Roland
R
Tron
François
F
Gilbert
Danièle
D
D000818:Animals; D017153:Antibodies, Anticardiolipin / Q000097:blood*; D004195:Disease Models, Animal; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009687:Nuclear Proteins / Q000138:chemical synthesis / Q000276:immunology*; D000090243:Nucleophosmin; D011994:Recombinant Proteins / Q000138:chemical synthesis / Q000276:immunology
D017153:Antibodies, Anticardiolipin; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D000090243:Nucleophosmin