title: Assignment of immune-related genes to the channel catfish, Ictalurus punctatus, genetic map.
abstract: Eighteen new genes, adenosine A1 receptor (ADORA1), complement component 4-beta (C4b), complement component 8-beta (C8b), chemokine ligand 19 (CCL19), chemokine ligand 21 (CCL21), chemokine ligand 25 (CCL25), chemokine receptor 2 (CCR2), chemokine receptor 5 (CCR5), chemokine receptor 4 (CCR4), chemokine receptor 7 (CCR7), chemokine receptor 9 (CCR9), interleukin 1-beta (IL1B), integrin II-beta (ITGB2), novel immune type receptor 2 (NITR2), novel immune type receptor 4 (NITR4), natural killer cell lysin (NKLYSIN), nucleotide excision repair (RAD23B) and tumour necrosis factor-alpha (TNF), were assigned to the channel catfish (Ictalurus punctatus) genetic linkage map. Polymorphic microsatellite markers were developed for NITR2, NITR4 and RAD23B from short-tandem repeats in the available sequence. Polymorphic microsatellite markers were developed for the remaining 15 genes by short-tandem repeat-anchored primer sequencing of catfish bacterial artificial chromosomes. Two gene clusters (MYOG-NRAMP-ADORA1) and (CCR4-CCR2-CCR5) displayed conservation of synteny between catfish and mammals. Assignment of 18 new genes to the catfish linkage map will further advance integration of genetic and physical maps and comparative mapping between channel catfish and map rich species.
authors.affiliation: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, PO Box 6100, Mississippi State, MS 39762-6100, USA.
authors.forename: A
authors.initials: A
authors.lastname: Karsi
authors.forename: W R
authors.initials: WR
authors.lastname: Wolters
authors.forename: G C
authors.initials: GC
authors.lastname: Waldbieser
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D017931:DNA Primers; D005796:Genes / Q000235:genetics* / Q000276:immunology; D007059:Ictaluridae / Q000235:genetics* / Q000276:immunology*; D018895:Microsatellite Repeats / Q000235:genetics; D026801:Synteny / Q000235:genetics
chemical_list: D017931:DNA Primers