Cutaneous distribution and localization of dyes affected by vehicles all with different lipophilicity.
The penetration of topically applied substances into the stratum corneum (SC) depends on several factors, e.g., the physicochemical properties of the vehicle used for application. The penetration of highly hydrophilic and lipophilic dyes into the skin was studied using a pure oil (o) or water (w) for the application compared to an o/w emulsion. The penetration and localization of both dyes, the lipophilic curcumin and the hydrophilic Patent blue V, was investigated in vivo using the method of tape stripping and microscopy. In addition, histological sections of biopsies, removed from porcine ear skin were studied using microscopy. Differences in the distribution and the localization of both dyes within the SC were observed. These differences depend on the physicochemical properties of both the vehicles and the dyes. The vehicle appears to affect, in particular, the pathways of penetration.
Jacobi
Ute
U
Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Medical Faculty Charité, Universitätsmedizin Berlin, 10098 Berlin, Germany. ute.jacobi@charite.de
Tassopoulos
Tatiana
T
Surber
Christian
C
Lademann
Jürgen
J
D000279:Administration, Cutaneous; D000328:Adult; D000818:Animals; D002626:Chemistry, Pharmaceutical; D003474:Curcumin / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D006651:Histocytochemistry; D006801:Humans; D008055:Lipids / Q000032:analysis*; D008297:Male; D009821:Oils / Q000032:analysis; D010539:Permeability; D014677:Pharmaceutical Vehicles; D012394:Rosaniline Dyes / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D012867:Skin / Q000737:chemistry*; D012869:Skin Absorption; D012879:Skin Physiological Phenomena; D013552:Swine; D014867:Water / Q000032:analysis
D008055:Lipids; D009821:Oils; D014677:Pharmaceutical Vehicles; D012394:Rosaniline Dyes; D014867:Water; C008769:sulfan blue; D003474:Curcumin