title: Nanosphere induced gene expression in human dendritic cells.
abstract: The molecular mechanisms of nanosphere-induced mucosal immunization are important to decipher, since this can form the basis for novel approaches in, e.g., nasal vaccination. In this study, we have investigated the effect of nanospheres as antigen carriers on immature human dendritic cells. The results clearly indicate that tetanus toxoid immobilized nanospheres have a direct effect on human monocyte derived dendritic cells and induce a specific transcriptional profile involving genes crucial for phagocytosis and a protective immune response.
authors.affiliation: Department of Molecular Chemistry, Graduate School of Engineering, Osaka University and CREST, Osaka, Japan.
authors.forename: Michiya
authors.initials: M
authors.lastname: Matsusaki
authors.forename: Kristina
authors.initials: K
authors.lastname: Larsson
authors.forename: Takani
authors.initials: T
authors.lastname: Akagi
authors.forename: Malin
authors.initials: M
authors.lastname: Lindstedt
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Akashi
authors.forename: Carl A K
authors.initials: CA
authors.lastname: Borrebaeck
mesh_terms: D051940:B7-2 Antigen / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D004337:Drug Carriers; D015870:Gene Expression; D020869:Gene Expression Profiling; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D018928:Immunity, Mucosal; D066298:In Vitro Techniques; D043942:Nanotubes; D010587:Phagocytosis; D013745:Tetanus Toxoid / Q000008:administration & dosage
chemical_list: D051940:B7-2 Antigen; D004337:Drug Carriers; D006684:HLA-DR Antigens; D013745:Tetanus Toxoid