[The expression of recombinant protein of human interleukin-24 and its anti-tumor mechanism].
The hIL24 cDNA sequence was cloned into prokaryotic high expressive vector pET-21a(+) and recombinant hIL24 was expressed in E. coli with IPTG induction. The purified recombinant hIL24 exhibits following functions in HeLa cell: inhibiting cell growth, inducing apoptosis, inducing PMBC to secrete IL-6, TNF-alpha, IFN-r and inhibiting blood vessel formation. Our preliminary results suggest that the apoptosis induced by rhIL24 is through down-regulating expression of anti-apoptosis factor Bcl-2 and activation of mitochondria apoptosis pathway.
Ye
Zhen-Min
ZM
Cell and Molecular Biology Institute, College of Medicine, SooChow University, Suzhou 215007, China.
Zhu
Ye-Han
YH
Sheng
Wei-Hua
WH
Miao
Jing-Cheng
JC
Wei
Shao-Hua
SH
Wu
Kang
K
Yang
Ji-Cheng
JC
D017209:Apoptosis / Q000187:drug effects*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D008928:Mitochondria / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D000099180:Interleukin-24
D007378:Interleukins; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; D000099180:Interleukin-24