Identification and characterization of Sarcoptes scabiei and Dermatophagoides pteronyssinus glutathione S-transferases: implication as a potential major allergen in crusted scabies.
The astigmatid mite Sarcoptes scabiei is the causative agent of scabies, a highly infectious parasitic disease of the skin. Although the mite causes marked hypersensitivity reactions, particularly in crusted (severe) scabies, little is known about the specific scabies mite molecules involved in such immunologic responses. We have identified six genes encoding scabies mite homologues of mu and delta-like glutathione S-transferases (GSTs) as well as novel house dust mite GSTs. A mu class S. scabiei GST was subcloned into a prokaryotic expression system. The purified recombinant protein rSsGST01 reacted strongly with IgE and IgG4 in sera from crusted scabies patients. This response was not observed with control antigens or with ordinary scabies and uninfested patient sera. In addition, the specific IgE response to rSsGST01 did not correlate with the total IgE level of the patient. These results suggest that GST may play a role in the pathophysiology associated with crusted scabies.
Dougall
Annette
A
Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Darwin, Northern Territory, Australia.
Holt
Deborah C
DC
Fischer
Katja
K
Currie
Bart J
BJ
Kemp
David J
DJ
Walton
Shelley F
SF
D000485:Allergens / Q000276:immunology*; D000818:Animals; D040002:Dermatophagoides pteronyssinus / Q000201:enzymology* / Q000276:immunology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D012520:Sarcoptes scabiei / Q000201:enzymology* / Q000276:immunology; D012532:Scabies / Q000276:immunology*; D017422:Sequence Analysis, DNA
D000485:Allergens; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D005982:Glutathione Transferase