title: Implementation of the modified-SHIRPA protocol for screening of dominant phenotypes in a large-scale ENU mutagenesis program.
abstract: SHIRPA is a three-stage protocol for the comprehensive assessment of primarily mouse behavior. The first stage consists of high-throughput phenotyping of 33 behavioral observations and 7 metabolic or disease observations. We modified this part of the protocol by integrating new morphologic observations into the initial phenotype assay of behavior and dysmorphology. Behavioral observations assessed by this protocol, now referred to as the "modified-SHIRPA," are compatible with the original "SHIRPA" protocol. Using modified-SHIRPA, we screened dominant phenotypes of more than 10,000 G(1) progeny generated by crossing DBA/2J females with ENU-treated C57BL/6J males. To date, we have obtained 136 hereditary-confirmed mutants that exhibit behavioral and morphologic defects. Some independent mutant lines exhibited similar phenotypes, suggesting that they may represent alleles of the same gene or mutations in the same genetic pathway. They could hold great potential for the unraveling of the molecular mechanisms of certain phenotypes.
authors.affiliation: Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Kouyadai, Tsukubai, Ibaraki, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Masuya
authors.forename: Maki
authors.initials: M
authors.lastname: Inoue
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Wada
authors.forename: Aya
authors.initials: A
authors.lastname: Shimizu
authors.forename: Junko
authors.initials: J
authors.lastname: Nagano
authors.forename: Akiko
authors.initials: A
authors.lastname: Kawai
authors.forename: Ayako
authors.initials: A
authors.lastname: Inoue
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kagami
authors.forename: Taeko
authors.initials: T
authors.lastname: Hirayama
authors.forename: Ayako
authors.initials: A
authors.lastname: Yamaga
authors.forename: Hideki
authors.initials: H
authors.lastname: Kaneda
authors.forename: Kimio
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Osamu
authors.initials: O
authors.lastname: Minowa
authors.forename: Ikuo
authors.initials: I
authors.lastname: Miura
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Gondo
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Noda
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Wakana
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Shiroishi
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D005038:Ethylnitrosourea / Q000494:pharmacology*; D005260:Female; D006614:Hindlimb / Q000002:abnormalities; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008817:Mice, Mutant Strains / Q000145:classification / Q000235:genetics; D016296:Mutagenesis; D009153:Mutagens; D010641:Phenotype; D012880:Skin Pigmentation / Q000235:genetics
chemical_list: D009153:Mutagens; D005038:Ethylnitrosourea