Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas.
An actinobacterial strain, RMV-1378T, isolated from a cold desert of the Indian Himalayas, was subjected to polyphasic taxonomic characterization. The strain formed branching, non-fragmenting vegetative hyphae and did not produce diffusible pigments. Neither aerial mycelium nor spore formation was observed. The G+C content of the DNA was 72.0 mol%. The strain had chemotaxonomic characteristics typical of the genus Actinoalloteichus and was closely related (99.3 % 16S rRNA gene sequence similarity) to Actinoalloteichus cyanogriseus, currently the only Actinoalloteichus species with a validly published name. However, the results of DNA-DNA hybridization experiments showed 51.9 % relatedness with the type strain of A. cyanogriseus. On the basis of the above data and the physiological and biochemical distinctiveness of RMV-1378T (=MTCC 6194T=JCM 12472T=DSM 44848T), this strain should be classified as the type strain of a novel species of Actinoalloteichus, for which the name Actinoalloteichus spitiensis sp. nov. is proposed.
Singla
A K
AK
Biochemical Engineering Research and Process Development Centre (BERPDC) and Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Council of Scientific and Industrial Research (CSIR), Sector 39-A, Chandigarh 160 036, India.
Mayilraj
S
S
Biochemical Engineering Research and Process Development Centre (BERPDC) and Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Council of Scientific and Industrial Research (CSIR), Sector 39-A, Chandigarh 160 036, India.
Kudo
Takuji
T
Japan Collection of Microorganisms (JCM), RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Krishnamurthi
S
S
Biochemical Engineering Research and Process Development Centre (BERPDC) and Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Council of Scientific and Industrial Research (CSIR), Sector 39-A, Chandigarh 160 036, India.
Prasad
G S
GS
Biochemical Engineering Research and Process Development Centre (BERPDC) and Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Council of Scientific and Industrial Research (CSIR), Sector 39-A, Chandigarh 160 036, India.
Vohra
R M
RM
Biochemical Engineering Research and Process Development Centre (BERPDC) and Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Council of Scientific and Industrial Research (CSIR), Sector 39-A, Chandigarh 160 036, India.
D000192:Actinomycetales / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D003889:Desert Climate; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S