Inhibitory effect of deferoxamine on Paracoccidioides brasiliensis survival in human monocytes: reversal by holotransferrin not by apotransferrin.
The mechanisms used by Paracoccidioides brasiliensis to survive into phagocytic cells are not clear. Cellular iron metabolism is of critical importance to the growth of several intracellular pathogens whose capacity to multiply in mononuclear phagocytes is dependent on the availability of intracellular iron. Thus, the objective of this paper was to investigate the role of intracellular iron in regulating the capacity of P. brasiliensis yeast cells to survive within human monocytes. Treatment of monocytes with deferoxamine, an iron chelator, suppressed the survival of yeasts in a concentration-dependent manner. The effect of deferoxamine was reversed by iron-saturated transferrin (holotransferrin) but not by nonsaturated transferrin (apotransferrin). These results strongly suggest that P. brasiliensis survival in human monocytes is iron dependent.
Dias-Melicio
Luciane Alarcão
LA
Department of Microbiology and Immunology, Biosciences Institute, São Paulo State University, Botucatu, SP, Brazil. acsoares@ibb.unesp.br
Calvi
Sueli Aparecida
SA
Peraçoli
Maria Terezinha Serrão
MT
Soares
Angela Maria Victoriano de Campos
AM
D001059:Apoproteins / Q000494:pharmacology*; D003676:Deferoxamine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006801:Humans; D007501:Iron / Q000502:physiology; D009000:Monocytes / Q000382:microbiology*; D010228:Paracoccidioides / Q000187:drug effects* / Q000502:physiology; D017262:Siderophores / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014168:Transferrin / Q000494:pharmacology*
D001059:Apoproteins; D017262:Siderophores; D014168:Transferrin; C028284:apotransferrin; C110714:holotransferrin; D007501:Iron; D003676:Deferoxamine