The role of innate immune cells in the response of heat-treated Mycobacterium tuberculosis (M.tb) antigens stimulating PBMCs.
The proliferation response of gammadelta T cells to the antigen from heat-treated Mycobacterium tuberculosis H37Ra (M.tb Ag) was used as a good model in gammadeltaT cell research. From preliminary research it is found that activated NK cells positively elevated gammadeltaT cells proliferation after simulating PBMCs with M.tb Ag. To investigate different behaviors of NK cells, gammadeltaNKT cells, gammadeltaT cells and relationships between these cell subsets, activation and proliferation of different cell subsets of PBMCs in response to M.tb Ag were analyzed. We demonstrated that NK cells, gammadeltaNKT cells and gammadeltaT cells could be activated after stimulation with M.tb Ag. gammadeltaNKT cells and gammadelta T cells proliferated while the number of NK cells decreased after 11 day-simulation with M.tb Ag. Meanwhile, at the early time of stimulation the cytotoxicity of PBMCs was enhanced.
Wang
Chao
C
Institute of Immunology, School of Life Sciences, University of Science & Technology of China, Hefei, China.
Li
Jun
J
Zheng
Huaixin
H
Wei
Haiming
H
Zhang
Ruijun
R
Li
Baiqing
B
Tian
Zhigang
Z
D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D006358:Hot Temperature; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology*; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011489:Protein Denaturation; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013997:Time Factors; D015854:Up-Regulation
D000942:Antigens, Bacterial