Analysis of recQ gene transcript in fruiting bodies of basidiomycetous mushroom Lentinula edodes.
Quantitative reverse transcription PCR analysis and total RNA staining demonstrated that Lentinula edodes recQ gene (Le.recQ) transcript is present in all the parts of the fruiting body, but in hymenophore at the highest density. Results of in situ RNA-RNA hybridization showed that the Le.recQ transcript level within the hymenophore is higher in the hymenium, subhymenium, and the outer region of the trama. Trama cells themselves contain a lower level of the transcript.
Katsukawa
Shiho
S
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan. shihokat@bio.titech.ac.jp
Shishido
Kazuo
K
D000251:Adenosine Triphosphatases / Q000235:genetics*; D001487:Basidiomycota; D004265:DNA Helicases / Q000235:genetics*; D048690:Fruiting Bodies, Fungal / Q000201:enzymology / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D053484:RecQ Helicases; D020942:Shiitake Mushrooms / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D013194:Staining and Labeling
D012333:RNA, Messenger; D000251:Adenosine Triphosphatases; D004265:DNA Helicases; D053484:RecQ Helicases