title: Up-regulation of the claudin-6 gene in adipogenesis.
abstract: To investigate the role of claudin-6 in adipogenesis, claudin-6 mRNA was examined in adipose tissues and adipocyte differentiation. Claudin-6 mRNA was found to be differentially expressed in four different adipose tissues, and up-regulated in each fat depot of mice fed a high-fat diet as compared to a normal-fat diet. Levels of claudin-6 transcripts were increased during differentiation of 3T3-L1 cells in vitro. Moreover, small interfering RNA (siRNA)-mediated reduction of claudin-6 mRNA inhibited differentiation of 3T3-L1 cells. These results suggest that claudin-6 is another important regulator in adipogenesis and fat deposition.
authors.affiliation: Department of Food Production Science, Faculty of Agriculture, Shinshu University, Nagano, Japan.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Hong
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hishikawa
authors.forename: Hisae
authors.initials: H
authors.lastname: Miyahara
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tsuzuki
authors.forename: Chizu
authors.initials: C
authors.lastname: Gotoh
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Iga
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Sang-Houn
authors.initials: SH
authors.lastname: Song
authors.forename: Ki-Choon
authors.initials: KC
authors.lastname: Choi
authors.forename: Hong-Gu
authors.initials: HG
authors.lastname: Lee
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sasaki
authors.forename: Sang-Gun
authors.initials: SG
authors.lastname: Roh
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000235:genetics*; D000273:Adipose Tissue / Q000737:chemistry / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D057167:Claudins; D004041:Dietary Fats / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis; D034741:RNA, Small Interfering / Q000494:pharmacology; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D057167:Claudins; D004041:Dietary Fats; D008565:Membrane Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C122101:claudin 6