Relation between oxidative stress markers and antioxidant endogenous defences during exhaustive exercise.
Hydrogen peroxide (H2O2) could induce oxidative damage at long distance from its generation site and it is also an important signalling molecule that induces some genes related to oxidative stress. Our objective was to study the plasma and blood cells capability to detoxify H2O2 after intense exercise and its correlation with oxidative damage. Blood samples were taken from nine professional cycling, participating in a mountain stage, under basal conditions and 3 h after the competition. Catalase and glutathione peroxidase activities decreased (40 and 50% respectively) in neutrophils after the cycling stage, while glutathione peroxidase increased (87%) in lymphocytes. Catalase protein levels and catalase specific activity maintained basal values after the stage in plasma. Catalase protein levels decreased (48%) in neutrophils and its specific activity increased up to plasma values after exercise. Myeloperoxidase (MPO) increased (39%) in neutrophils after the cycling stage. Exercise-induced hemolysis and lymphopenia inversely correlated with cellular markers of oxidative stress. Plasma malondialdehyde (MDA) directly correlated with neutrophil MPO activity and erythrocytes MDA. Intense exercise induces oxidative damage in blood cells as erythrocytes and lymphocytes, but not in neutrophils.
Sureda
Antoni
A
Laboratori de Ciències de l'Activitat Física, Departament de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Crtra. Valldemossa Km 7.5. E-07122-Palma de Mallorca, Illes Balears, Spain.
Tauler
Pedro
P
Aguiló
Antoni
A
Cases
Nuria
N
Fuentespina
Emilia
E
Córdova
Alfredo
A
Tur
Josep A
JA
Pons
Antoni
A
D000975:Antioxidants / Q000378:metabolism*; D001642:Bicycling; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood; D006461:Hemolysis; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D018384:Oxidative Stress; D009195:Peroxidase / Q000097:blood; D010807:Physical Endurance
D000975:Antioxidants; D015415:Biomarkers; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D005979:Glutathione Peroxidase