Expressed protein ligation to study protein interactions: semi-synthesis of the G-protein alpha subunit.
Interactions between G proteins and GPCRs are fundamental for transmitting signals for a multitude of physiologic responses. Little is known regarding the protein-protein interface between the G protein and the receptor, much less the mechanisms for receptor activation of G proteins. Here, we will describe how expressed protein ligation will aid in the study of protein-protein interactions between semi-synthetic G alpha subunits and GPCRs.
Anderson
Lori L
LL
Department of Biochemistry and Molecular Biophysics, Washington University, St. Louis, MO 63110, USA.
Marshall
Garland R
GR
Baranski
Thomas J
TJ
D000818:Animals; D044385:GTP-Binding Protein alpha Subunits / Q000138:chemical synthesis*; D019204:GTP-Binding Proteins / Q000737:chemistry; D006801:Humans; D047668:Inteins; D008956:Models, Chemical; D015202:Protein Engineering / Q000379:methods; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*
D044385:GTP-Binding Protein alpha Subunits; D011506:Proteins; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins