Isolation and expression pattern of RGS21 gene, a novel RGS member.
Regulators of G-protein signaling (RGS) proteins are known for the RGS domain that is composed of a conserved stretch of 120 amino acids, which binds directly to activated G-protein alpha subunits and acts as a GTPase-activating protein (GAP), leading to their deactivation and termination of downstream signals. In this study, a novel human RGS cDNA (RGS21), 1795 bp long and encoding a 152-amino acid polypeptide, was isolated by large-scale sequencing analysis of a human fetal brain cDNA library. Unlike other RGS family members, RGS21 gene has no additional domain/motif and may represent the smallest known member of RGS family. It may belong to the B/R4 subfamily, which suggests that it may serve exclusively as a negative regulator of alphai/o family members and/or alphaq/11. PCR analysis showed that RGS21 mRNA was expressed ubiquitously in the 16 tissues examined, implying general physiological roles.
Li
Xin
X
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.
Chen
Lei
L
Ji
Chaoneng
C
Liu
Bing
B
Gu
Jiefeng
J
Xu
Jian
J
Zou
Xianqiong
X
Gu
Shaohua
S
Mao
Yumin
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D020555:GTP-Binding Protein Regulators / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D020710:RGS Proteins; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment
D018076:DNA, Complementary; D020555:GTP-Binding Protein Regulators; D020710:RGS Proteins; C512065:RGS21 protein, human; D012333:RNA, Messenger