title: Phytoestrogens and their metabolites inhibit the sensitivity of the bovine corpus luteum to luteotropic factors.
abstract: The aim of this study was to examine whether active metabolites of phytoestrogens (equol and para-ethyl-phenol) inhibit sensitivity of bovine corpus luteum (CL) to luteinizing hormone (LH) and to auto/paracrine luteotropic factors (prostaglandin E2-PGE2 and prostaglandin F(2alpha)-PGF(2alpha)), and whether they influence pulsatile progesterone (P4) secretion by the bovine CL. In in vivo experiments, high levels of equol and para-ethyl-phenol were found in plasma and in the CL tissue of heifers and cows fed a soy bean diet (2.5 kg/animal/day), along with lower concentrations of P4 (P < 0.05). Both Prostaglandins (PG) and LH strongly stimulated P4 secretion in cultured pieces of CL that were collected from cows fed a standard diet (P < 0.01). There was no effect of PGs and LH on P4 stimulation in CLs obtained from cows fed a diet rich in soy bean. Finally, we examined whether active metabolites of phytoestrogens participated in regulation of pulsatile P4 secretion and LH-stimulated P4 secretion in vitro using a microdialysis system. Equol and para-ethyl-phenol had no effect on basic and pulsatile P4 secretion in CLs during 240 min of perfusion when compared to the control (P < 0.05). However, they inhibited LH-stimulated P4 secretion (P < 0.05). Phytoestrogens and their metabolites may disrupt CL function by inhibiting PG- and LH-stimulated P4 secretion.
authors.affiliation: Department of Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
authors.forename: Katarzyna K
authors.initials: KK
authors.lastname: Piotrowska
authors.forename: Izabela
authors.initials: I
authors.lastname: Woclawek-Potocka
authors.forename: Mamadou M
authors.initials: MM
authors.lastname: Bah
authors.forename: Mariusz K
authors.initials: MK
authors.lastname: Piskula
authors.forename: Wojciech
authors.initials: W
authors.lastname: Pilawski
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Bober
authors.forename: Dariusz J
authors.initials: DJ
authors.lastname: Skarzynski
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000187:drug effects* / Q000378:metabolism; D060754:Equol; D005260:Female; D066298:In Vitro Techniques; D007529:Isoflavones / Q000097:blood; D007986:Luteinizing Hormone / Q000502:physiology; D017551:Microdialysis; D009132:Muscles / Q000378:metabolism; D010636:Phenols / Q000097:blood; D048789:Phytoestrogens / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D011374:Progesterone / Q000097:blood / Q000378:metabolism*; D013025:Glycine max
chemical_list: C007610:4',7-dihydroxy-3,4-dihydroisoflavone; D007529:Isoflavones; D010636:Phenols; D048789:Phytoestrogens; D011374:Progesterone; D060754:Equol; D007986:Luteinizing Hormone; C042291:4-ethylphenol