title: T lymphocytes and dendritic cells are activated by the deletion of peroxiredoxin II (Prx II) gene.
abstract: Peroxiredoxin II (Prx II) is a member of antioxidant enzyme family and it plays a protective role against oxidative damage. Constitutive production of endogenous reactive oxygen species was detected in spleen and bone marrow cells lacking Prx II. Here, we investigated the role of Prx II in immune responses. The total number of splenocytes (especially, the population of S-phase cells and CD3(+) T cells) was significantly higher in Prx II(-/-) mice than in wild type. Number of peripheral blood mononuclear cells (PBMCs) in Prx II(-/-) mice was also higher than wild type. Differentiation of Prx II(-/-) mouse bone marrow cells into CD11c-positive dendritic cells was greater than that of wild type. Transplantation of Prx II(-/-) bone marrow cells into wild type mice increased PBMCs in blood and bone marrow-derived dendritic cells. Prx II deletion enhances concanavalin A (ConA)-induced splenocyte proliferation and mixed lymphocyte reaction (MLR) activity of bone marrow-derived CD11c-positive dendritic cells to stimulate recipient splenocytes. Collectively, these data suggest that Prx II inhibits the immune cell responsiveness, which may be regulated by scavenging the low amount of reactive oxygen species (ROS).
authors.affiliation: Laboratory of Human Genomics, Korea Research Institute of Bioscience and Biotechnology, Taejeon 305-806, Republic of Korea. eunyi@kribb.re.kr
authors.forename: Eun-Yi
authors.initials: EY
authors.lastname: Moon
authors.forename: Young-Wook
authors.initials: YW
authors.lastname: Noh
authors.forename: Ying-Hao
authors.initials: YH
authors.lastname: Han
authors.forename: Sun-Uk
authors.initials: SU
authors.lastname: Kim
authors.forename: Jin-Man
authors.initials: JM
authors.lastname: Kim
authors.forename: Dae-Yeul
authors.initials: DY
authors.lastname: Yu
authors.forename: Jong-Seok
authors.initials: JS
authors.lastname: Lim
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D010544:Peroxidases / Q000235:genetics* / Q000502:physiology; D054464:Peroxiredoxins; D017382:Reactive Oxygen Species / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D017382:Reactive Oxygen Species; D010544:Peroxidases; D054464:Peroxiredoxins