[Production of a novel heparinase from Sphingobacterium sp].
The novel heparinase-producing bacterial strain Sphingobacterium sp. was isolated and screened from soil. The optimum medium composition is (g/L): Soytone 20, NaCl 1, K2HPO4 2.5, MgSO4 0.5, Heparin 2, Sucrose 15, pH 7.5. The optimum temperature for growth and enzyme production was 32 degrees C. When cultured at a rotating shaker at 30 degrees C for 36 hours, 200 r/min, 50 mL medium in 500 mL flask, the production of heparinase reached 4000 U/L.
Gao
Ningguo
N
Institute of Microbiology, Chinese academy of Sciences, Beijing 100080, China.
Cheng
Xiulan
X
Yang
Jing
J
Zhang
Shuzheng
S
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D019763:Heparin Lyase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012988:Soil Microbiology; D045249:Sphingobacterium / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D013696:Temperature
D001426:Bacterial Proteins; D019763:Heparin Lyase