Accelerated solvent extraction of ochratoxin a from rice samples.
In this paper, accelerated solvent extraction (ASE) for the analysis of ochratoxin A (OTA) is applied for the first time. Optimization of the method is given for the extraction of OTA from rice samples. Several parameters such as type of solvent, temperature, pressure, static time, and cell size were investigated thoroughly to find the optimal extraction conditions. The optimum ASE operating conditions were methanol as extraction solvent, 1500 psi, 40 degrees C, 5 min of static time, 50% flush volume, 60 s of purge, 1 cycle, and 11 mL cell size. The total extraction time was approximately 15 min. OTA was determined by liquid chromatography coupled with a fluorescence detector and confirmed by methyl ester derivatization. The analytical performance of the method was monitored with quality control parameters. Finally, the optimized method was used to evaluate 12 rice samples, 1 of which was positive with an OTA content of 4.17 ng/g. The proposed method offers the possibility of a fast and simple process to obtain a quantitative extraction of OTA.
Juan
Cristina
C
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Avenida Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
González
Liliana
L
Soriano
Jose Miguel
JM
Moltó
Juan Carlos
JC
Mañes
Jordi
J
D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D009793:Ochratoxins / Q000032:analysis / Q000302:isolation & purification*; D012275:Oryza / Q000737:chemistry*; D011312:Pressure; D012997:Solvents; D013696:Temperature
D009793:Ochratoxins; D012997:Solvents; C025589:ochratoxin A