title: Fiber-optic fluorescence imaging.
abstract: Optical fibers guide light between separate locations and enable new types of fluorescence imaging. Fiber-optic fluorescence imaging systems include portable handheld microscopes, flexible endoscopes well suited for imaging within hollow tissue cavities and microendoscopes that allow minimally invasive high-resolution imaging deep within tissue. A challenge in the creation of such devices is the design and integration of miniaturized optical and mechanical components. Until recently, fiber-based fluorescence imaging was mainly limited to epifluorescence and scanning confocal modalities. Two new classes of photonic crystal fiber facilitate ultrashort pulse delivery for fiber-optic two-photon fluorescence imaging. An upcoming generation of fluorescence imaging devices will be based on microfabricated device components.
authors.affiliation: James H. Clark Center for Biomedical Engineering and Sciences, Stanford University, 318 Campus Drive, Stanford, California 94305, USA.
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Flusberg
authors.forename: Eric D
authors.initials: ED
authors.lastname: Cocker
authors.forename: Wibool
authors.initials: W
authors.lastname: Piyawattanametha
authors.forename: Juergen C
authors.initials: JC
authors.lastname: Jung
authors.forename: Eunice L M
authors.initials: EL
authors.lastname: Cheung
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Schnitzer
mesh_terms: D000818:Animals; D005336:Fiber Optic Technology / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D055100:Optical Fibers