Proteomic analysis of the Gallus gallus embryo at stage-29 of development.
The chicken (Gallus gallus) is one of the primary models for embryological and developmental studies. In order to begin to understand the molecular mechanisms underlying the normal and abnormal development of the chicken, we used 2-DE to construct a whole-embryo proteome map. Proteins were separated by IEF on IPG strips, and by 11% SDS-PAGE) gels. Protein identification was performed by means of PMF with MALDI-TOF-MS. In all, 105 protein spots were identified, 35 of them implicated in embryo development, 10 related with some diseases, and 16, finally, being proteins that have never been identified, purified or characterized in the chicken before. This map will be updated continuously and will serve as a reference database for investigators, studying changes at the protein level under different physiological conditions.
Agudo
David
D
Departamento de Ciencias de la Salud III, Facultad de Ciencias de la Salud, Universidad Rey Juan Carlos, Avenida de Atenas, S/N E28922 Alcorcón, Madrid, Spain. david.agudo@urjc.es
Agudo Garcillán
David
D
Gómez-Esquer
Francisco
F
Díaz-Gil
Gema
G
Martínez-Arribas
Fernando
F
Delcán
José
J
Schneider
José
J
Palomar
María Angustias
MA
Linares
Rafael
R
D000818:Animals; D002642:Chick Embryo / Q000254:growth & development* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D020543:Proteome / Q000032:analysis*
D020543:Proteome