[Oxidative stress-induced differentiation of L6 myoblasts].
OBJECTIVE
To explore the relationship between the differentiation of L6 myoblasts and oxidative stress.


METHODS
MTT assay was used to determine the viability of L6 myoblasts, from which the total RNA was extracted for amplification of the myogenin gene fragment by RT-PCR. H(2)O(2)-induced morphological changes of the cells were observed.


RESULTS
The myoblasts treated with low concentration of reactive oxygen (50 micromol/L H(2)O(2)) for 1 h exhibited accelerated cell growth (P<0.05), and treatment with 50 and 150 micromol/L H(2)O(2) induced the gene expression of myogenin, a molecular marker for differentiation of myoblasts. Morphological study revealed myotube formation and accelerated differentiation of the myoblasts induced by H(2)O(2).


CONCLUSIONS
The reactive oxygen may serve as the intracellular signal molecules to induce the growth and differentiation of the myoblasts.
Qiu
Xiao-zhong
XZ
Institute of Clinical Anatomy, Southern Medical University, Guangzhou 510515, China. qqiuxzh@yahoo.com.cn
Yu
Lei
L
Liao
Hua
H
Zhang
Li-sheng
LS
Qin
Jian-qiang
JQ
Yang
Jun
J
Ouyang
Jun
J
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D032446:Myoblasts / Q000166:cytology*; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats
D006861:Hydrogen Peroxide