Measurement of the total antioxidant response using a novel automated method in subjects with nonalcoholic steatohepatitis.
BACKGROUND
Oxidative stress, an increase in oxidants and/or a decrease in antioxidant capacity, is one of the potential biochemical mechanisms involved in the pathogenesis of nonalcoholic steatohepatitis. We aimed to investigate the total antioxidant response using a novel automated method in nonalcoholic steatohepatitis subjects. As a reciprocal measure, we also aimed to determine total peroxide level in the same plasma samples.


METHODS
Twenty-two subjects with biopsy proven nonalcoholic steatohepatitis and 22 healthy controls were enrolled. Total antioxidant response and total peroxide level measurements were done in all participants. The ratio percentage of total peroxide level to total antioxidant response was regarded as oxidative stress index.


RESULTS
Total antioxidant response of subjects with nonalcoholic steatohepatitis was significantly lower than controls (p < 0.05), while mean total peroxide level and mean oxidative stress index were higher (all p < 0.05). In subjects with nonalcoholic steatohepatitis, fibrosis score was significantly correlated with total peroxide level, total antioxidant response and oxidative stress index (p < 0.05, r = 0.607; p < 0.05, r = -0.506; p < 0.05, r = 0.728, respectively). However, no correlation was observed between necroimflamatory grade and those oxidative status parameters (all p > 0.05).


CONCLUSIONS
Nonalcoholic steatohepatitis is associated with increased oxidant capacity, especially in the presence of liver fibrosis. The novel automated assay is a reliable and easily applicable method for total plasma antioxidant response measurement in nonalcoholic steatohepatitis.
Horoz
Mehmet
M
Department of Internal Medicine, Harran University, Sanliurfa, Turkey. mehmethoroz@yahoo.com
Bolukbas
Cengiz
C
Bolukbas
Fusun F
FF
Sabuncu
Tevfik
T
Aslan
Mehmet
M
Sarifakiogullari
Serpil
S
Gunaydin
Necla
N
Erel
Ozcan
O
D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D001331:Automation; D016022:Case-Control Studies; D003124:Colorimetry / Q000379:methods* / Q000592:standards; D005234:Fatty Liver / Q000097:blood* / Q000150:complications / Q000378:metabolism; D005260:Female; D006801:Humans; D008103:Liver Cirrhosis / Q000209:etiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010545:Peroxides / Q000097:blood*; D012720:Severity of Illness Index
D000975:Antioxidants; D010545:Peroxides