title: Unusual solute protection against pressure inactivation of yeast alcohol dehydrogenase.
abstract: A 3:1 combination of sucrose and glycine provides significantly greater protection against pressure-induced inactivation of yeast alcohol dehydrogenase than either solute alone. Trehalose, alone, gives much greater protection than sucrose alone, but not so in combination with glycine. These are striking new findings that cannot be accounted for by current theories of protein stabilization.
authors.affiliation: Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53705, USA.
authors.forename: Gene
authors.initials: G
authors.lastname: Kidman
authors.forename: Dexter B
authors.initials: DB
authors.lastname: Northrop
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D002241:Carbohydrates / Q000502:physiology; D004795:Enzyme Stability / Q000502:physiology; D006874:Hydrostatic Pressure; D011489:Protein Denaturation / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013997:Time Factors
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D000426:Alcohol Dehydrogenase