title: [Activating protein-1 members in response to changes of wall-shear stress in osteoblastic cells].
abstract: OBJECTIVE
  To observe activating protein-1 (AP- 1) members in response to changes of wall-shear stress in osteoblastic cells in vitro.
  
  
  METHODS
  Isolated and purified osteoblastic cells from the calvaria of newborn SD rats were cultured and subcultured. The third generation cells were subjected to wall-shear stress of 0.8 Pa, 1.2 Pa, 1.4 Pa and 1.6 Pa separately. Gene expression of the seven AP-1 members were studied before (0 h) and 10 min, 15 min, 30 min, 60 min after treated with wall-shear stress.
  
  
  RESULTS
  The expression of FosB, c-Fos, c-Jun, JunD and JunB mRNA increased transiently after application of 1.2 Pa wall-shear stress in osteoblastic cells compared to 0.8 Pa , 1.4 Pa and 1.6 Pa stress, and peaked at 15 min.
  
  
  CONCLUSIONS
  Mechanical environment changes in osteoblastic cells induced a dramatic induction of most of the AP-1 members.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital, School of Stomatology, Zhejiang University, Hangzhou 310006, China.
authors.forename: Fu-dong
authors.initials: FD
authors.lastname: Zhu
authors.forename: Shi-fang
authors.initials: SF
authors.lastname: Zhao
mesh_terms: D000818:Animals; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D018808:Transcription Factor AP-1
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D018808:Transcription Factor AP-1