Circulating microparticles are elevated in haemophiliacs and non-haemophilic individuals aged <18 years.
Platelet and cell stimulation lead to plasma membrane remodelling, resulting in phosphatidylserine (PS) externalisation in the outer leaflet of the membrane, associated with the shedding of PS-rich microparticles (MPs), and contributes to thrombin generation by promoting the assembly of coagulation enzyme complexes. A previous study assessed MP levels in haemophiliacs after haemostatic treatment. To further investigate in vivo membrane remodelling, MP levels and characteristics were studied in 79 haemophiliacs and in 62 non-haemophiliacs. Both groups showed heterogeneity in MP levels, with higher values in individuals <18 years. This finding should be considered when studying MP levels in individuals <18 years.
Proulle
Valérie
V
Service d'Hématologie Biologique, AP-HP, Hôpital Bicêtre, Faculté de Médecine Paris XI, Le Kremlin Bicêtre, France. valerie.proulle@bct.aphp.fr
Hugel
Bénédicte
B
Guillet
Benoit
B
Grunebaum
Lélia
L
Lambert
Thierry
T
Freyssinet
Jean-Marie
JM
Dreyfus
Marie
M
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D002462:Cell Membrane / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005164:Factor IX / Q000032:analysis; D005169:Factor VIII / Q000032:analysis; D006467:Hemophilia A / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010718:Phosphatidylserines / Q000097:blood; D015539:Platelet Activation
D010718:Phosphatidylserines; D005169:Factor VIII; D005164:Factor IX