title: Microsatellite polymorphism in Japanese mongrel dogs.
abstract: The genetic variability of 182 unrelated mongrel dogs living in various areas of Japan (from Hokkaido to Okinawa) was studied by collecting their blood. Ten microsatellite loci were chosen from different autosomal chromosomes. After combining a few rare adjoining alleles to allelic classes, it was confirmed that the Hardy-Weinberg equilibrium was attained in each locus. The polymorphic information contents (PICs) of the loci, Ren37A11, Ren48E01, AHTk253, ZuBeCa30, Ren277K09, Ren42N13, AHT130, PEZ03, PEZ12, and AHT121, were 0.58, 0.63, 0.67, 0.67, 0.68, 0.71, 0.79, 0.80, 0.80, and 0.80, and the power of discriminations (PDs) were 0.80, 0.85, 0.87, 0.88, 0.88, 0.89, 0.94, 0.94, 0.94, and 0.94, respectively. The combined mean exclusion chance (MEC) was 0.9995, indicating that these microsatellite loci are useful for kinship testing of Japanese dogs.
authors.affiliation: Laboratory of Biophysics, School of Medicine, Teikyo University, Tokyo, Japan.
authors.forename: Noboru
authors.initials: N
authors.lastname: Oishi
authors.forename: Mari
authors.initials: M
authors.lastname: Maeda
authors.forename: Koichi
authors.initials: K
authors.lastname: Makimura
authors.forename: Toshiko
authors.initials: T
authors.lastname: Sawaguchi
authors.forename: Makio
authors.initials: M
authors.lastname: Hayashiya
authors.forename: Takuya
authors.initials: T
authors.lastname: Kubo
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Michihiro
authors.initials: M
authors.lastname: Kasahara
mesh_terms: D000818:Animals; D004285:Dogs / Q000235:genetics*; D005787:Gene Frequency; D005828:Genetics, Population; D007564:Japan; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic