title: [Expression of L-type calcium channel alpha1C subunit in adult rat heart].
abstract: OBJECTIVE
  To investigate the expression and distribution of L-type calcium channel alpha1C subunits in adult rat heart.
  
  
  METHODS
  HE staining was applied on the frozen sections of adult rat heart to identify the sinoatrial node (SAN), atrioventricular node (AVN), and posterior nodal extension (PNE). The protein expression of L-type calcium channel alpha1C in adult rat heart and its cellular localization were examined by Western blotting and immunohistochemistry, respectively.
  
  
  RESULTS
  L-type calcium channel alpha1C subunit was immunolocalized on the membrane of the myocardial cells, and its expression increased gradually in the SAN, AVN, PNE, right atrium and right ventricle. The protein level of L-type calcium channel alpha1C in the AVN was similar to that in the PNE (P>0.05), and its level in the right atrium and ventricle were significantly higher than those in the SAN and AVN (P<0.01). The results of Western blotting were well consistent with the results of immunohistochemistry.
  
  
  CONCLUSIONS
  The expression of L-type calcium channel alpha1C subunit may play a role in the electrophysiological functions of the heart.
authors.affiliation: Department of Cardiology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. oy1114@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Ou
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Niu
authors.forename: Fu-xian
authors.initials: FX
authors.lastname: Ren
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Feng-dong
authors.initials: FD
authors.lastname: Ling
mesh_terms: D000818:Animals; D001283:Atrioventricular Node / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004594:Electrophysiology; D005260:Female; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012849:Sinoatrial Node / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D020746:Calcium Channels, L-Type