title: Surface-associated material from Porphyromonas gingivalis stimulates the release of nitric oxide by inducing expression of inducible nitric oxide synthase.
abstract: The purpose of this study was to examine the effects of surface-associated material (SAM) from Porphyromonas gingivalis, a major cause of inflammatory periodontal disease, on the production of nitric oxide (NO) and expression of inducible nitric oxide synthase (iNOS) in the murine macrophage cell line RAW264.7. We also attempted to throw light on the signaling mechanisms involved in P. gingivalis SAM-induced NO production. SAM from P. gingivalis 381 was obtained by saline extraction. NO production was assayed by measuring the accumulation of nitrite in culture supernatants. Western blot analysis of iNOS and analysis of reverse transcription-polymerase chain reaction (RT-PCR) products were carried out. We found that P. gingivalis SAM can induce iNOS expression and stimulate the release of NO without additional stimuli and demonstrated an important role of the transcription factor NF-kappaB and microtubule polymerization in NO production. The production of NO required L-arginine, protein tyrosine kinase, and protein kinase C. The ability of P. gingivalis SAM to promote the production of NO may be important in the pathogenesis of inflammatory periodontal disease.
authors.affiliation: Department of Periodontology, College of Dentistry, Pusan National University, 1-10 Ami-Dong, Seo-Ku, Busan 602-739, South Korea. sungjokim@pusan.ac.kr
authors.forename: Sung-Jo
authors.initials: SJ
authors.lastname: Kim
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Choi
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Cho
authors.forename: Ju-Youn
authors.initials: JY
authors.lastname: Lee
authors.forename: Jeom-Il
authors.initials: JI
authors.lastname: Choi
authors.forename: In-Soon
authors.initials: IS
authors.lastname: Choi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*
chemical_list: D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; C048022:substrate adhesion molecules; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II