title: Methanomethylovorans thermophila sp. nov., a thermophilic, methylotrophic methanogen from an anaerobic reactor fed with methanol.
abstract: A novel thermophilic, obligately methylotrophic, methanogenic archaeon, strain L2FAW(T), was isolated from a thermophilic laboratory-scale upflow anaerobic sludge blanket reactor fed with methanol as the carbon and energy source. Cells of strain L2FAW(T) were non-motile, irregular cocci, 0.7-1.5 mum in diameter and usually occurred singly (sometimes forming clusters of two or four cells). The cells stained Gram-negative and lysed immediately in 0.1 % (w/v) SDS. Growth was inhibited by chloramphenicol and tetracycline, but not by penicillin, bacitracin, spectinomycin, vancomycin or kanamycin. Methanol and mono-, di- and trimethylamine were used as substrates, but H2/CO2, formate, acetate, propanol, dimethyl sulfide and methanethiol were not. The temperature range for growth was 42-58 degrees C, with an optimum at 50 degrees C. The fastest growth was observed at a salinity below 100 mM NaCl; no growth occurred above 300 mM NaCl. The optimal pH for growth was 6.5; growth was observed from pH 5 to pH 7.5. The G+C content of the genomic DNA was 37.6 mol%. Analysis of the 16S rRNA gene sequence and the partial methyl-CoM reductase gene sequence revealed that the organism was phylogenetically closely related to Methanomethylovorans hollandica DMS1T (98 % similarity for the 16S rRNA gene sequence and 91 % similarity for the methyl-CoM reductase gene sequence). The DNA-DNA relatedness between L2FAW(T) and Methanomethylovorans hollandica DMS1T was 46 %. On the basis of these results, strain L2FAW(T) (=DSM 17232T=ATCC BAA-1173T) represents the type strain of a novel species, for which the name Methanomethylovorans thermophila sp. nov. is proposed.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
authors.forename: B
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Laboratory of Microbiology of Anthropogenic Environments, Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.|Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
authors.forename: S N
authors.initials: SN
authors.lastname: Parshina
authors.affiliation: Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
authors.forename: W
authors.initials: W
authors.lastname: van Doesburg
authors.affiliation: Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
authors.forename: B P
authors.initials: BP
authors.lastname: Lomans
authors.affiliation: Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
authors.forename: A J M
authors.initials: AJM
authors.lastname: Stams
mesh_terms: D000693:Anaerobiosis; D001483:Base Sequence; D019149:Bioreactors / Q000382:microbiology; D000432:Methanol / Q000378:metabolism*; D017019:Methanosarcinaceae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D013440:Sulfides / Q000378:metabolism*; D013696:Temperature
chemical_list: D012336:RNA, Ribosomal, 16S; D013440:Sulfides; D000432:Methanol