title: An early intestinal mucosal source of gamma interferon is associated with resistance to and control of Cryptosporidium parvum infection in mice.
abstract: Resistance to and control of Cryptosporidium parvum infection in mice in the absence of adaptive immunity appears to be gamma interferon (IFN-gamma) dependent. Using an IFN-gamma-neutralizing antibody in a murine model, we demonstrated increased susceptibility to infection within 24 h. We correlated this early resistance and control with increased mucosal expression of IFN-gamma and demonstrate that CD8+ T-cell receptor alphabeta intestinal intraepithelial lymphocytes express and secrete this cytokine shortly after infection. The rapid kinetics of IFN-gamma expression and secretion by naive CD8+ T cells in response to a protozoan pathogen have not previously been demonstrated.
authors.affiliation: Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center, Box 041, 750 Washington Street, Boston, MA 02111, USA. bleav@tufts-nemc.org
authors.forename: Brett A
authors.initials: BA
authors.lastname: Leav
authors.forename: Masaru
authors.initials: M
authors.lastname: Yoshida
authors.forename: Kathleen
authors.initials: K
authors.lastname: Rogers
authors.forename: Seth
authors.initials: S
authors.lastname: Cohen
authors.forename: Nihal
authors.initials: N
authors.lastname: Godiwala
authors.forename: Richard S
authors.initials: RS
authors.lastname: Blumberg
authors.forename: Honorine
authors.initials: H
authors.lastname: Ward
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000953:Antigens, Protozoan / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003457:Cryptosporidiosis / Q000276:immunology*; D016785:Cryptosporidium parvum; D004198:Disease Susceptibility; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D007421:Intestine, Small / Q000276:immunology / Q000473:pathology; D051379:Mice; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis
chemical_list: D000906:Antibodies; D000953:Antigens, Protozoan; D016693:Receptors, Antigen, T-Cell, alpha-beta; D007371:Interferon-gamma