The expression of minichromosome maintenance protein-2 in normal and abnormal megakaryocytes and comparison with the proliferative marker Ki-67.
The minichromosome maintenance (Mcm) and Cdc6 proteins are important regulators of eucaryotic DNA replication. In most normal tissues, a similar proportion of cells express Mcm-2 and Ki-67. The present study showed that in both normal and abnormal states, the proportion of megakaryocytes expressing Mcm-2 is roughly seven times as many as those that express Ki-67. This is likely to be related to the process of endomitosis and endoreduplication. We also demonstrated that a significantly lower proportion of megakaryocytes in myelodysplastic syndrome express Mcm-2. These findings provide new insights into megakaryocyte biology.
Lampert
Irvin A
IA
Department of Histopathology, Hammersmith Hospital, Imperial College London, London, UK. ialampert@blueyonder.co.uk
Horncastle
Donna
D
Dilworth
Steven
S
Roberts
Irene
I
Alison
Malcolm R
MR
Naresh
Kikkeri N
KN
D001853:Bone Marrow / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D015672:Erythroid Precursor Cells / Q000378:metabolism; D006801:Humans; D039661:Integrin beta3 / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism*; D008533:Megakaryocytes / Q000378:metabolism*; D064111:Minichromosome Maintenance Complex Component 2; D009190:Myelodysplastic Syndromes / Q000378:metabolism* / Q000473:pathology; D009196:Myeloproliferative Disorders / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000378:metabolism*
D018797:Cell Cycle Proteins; C061248:ITGB3 protein, human; D039661:Integrin beta3; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; C084859:MCM2 protein, human; D064111:Minichromosome Maintenance Complex Component 2