title: Heme oxygenase-1 (Hsp32) is involved in the protection of small intestine by whole body mild hyperthermia from ischemia/reperfusion injury in rat.
abstract: OBJECTIVE
  The aim of the present study was to explore whether heme oxygenase-1 (HO-1) is involved in the hyperthermia-provided protection of the small intestine from ischemia/reperfusion injury in rats.
  
  
  METHODS
  Intestinal damage was induced in male Sprague-Dawley rats by clamping both the superior mesenteric artery and the celiac trunk for 30 min, followed by reperfusion. Whole-body hyperthermia was induced in anesthetized rats by placement in a temperature-controlled water bath. Whole-body hyperthermia to a core temperature of 42-43 degrees C for 15 min was followed by passive cooling. We started the hyperthermic treatment 6 h before the vascular clamping. The severity of the mucosal injury was evaluated by several biochemical markers and histological findings. Hyperthermia-induced heat-shock proteins were detected by Western blotting. We also investigated the effect of zinc protoporphyrin IX (an HO-1 inhibitor) on the protective effect of hyperthermia.
  
  
  RESULTS
  The rats, which were killed after ischemia/reperfusion, had severe intestinal inflammation. Hyperthermia significantly induced the production of Hsp70 and HO-1 in intestinal mucosa and significantly reduced ischemia/reperfusion-induced mucosal injury. The combination of zinc protoporphyrin IX with hyperthermia extinguished the protective effects of hyperthermia on ischemia/reperfusion injury.
  
  
  CONCLUSIONS
  Hyperthermia protects against ischemia/reperfusion injury in rat small intestine through the expression of heat-shock proteins, especially HO-1.
authors.affiliation: Inflammation and Immunology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sakamoto
authors.forename: S
authors.initials: S
authors.lastname: Kokura
authors.forename: T
authors.initials: T
authors.lastname: Okuda
authors.forename: T
authors.initials: T
authors.lastname: Hattori
authors.forename: K
authors.initials: K
authors.lastname: Katada
authors.forename: Y
authors.initials: Y
authors.lastname: Isozaki
authors.forename: N
authors.initials: N
authors.lastname: Nakabe
authors.forename: O
authors.initials: O
authors.lastname: Handa
authors.forename: T
authors.initials: T
authors.lastname: Takagi
authors.forename: T
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Naito
authors.forename: N
authors.initials: N
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D019743:Chemokines, CXC / Q000032:analysis; D004790:Enzyme Induction; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis / Q000502:physiology*; D006979:Hyperthermia, Induced; D051550:I-kappa B Kinase / Q000096:biosynthesis; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis; D007413:Intestinal Mucosa / Q000737:chemistry / Q000187:drug effects; D007421:Intestine, Small / Q000166:cytology / Q000187:drug effects; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D011524:Protoporphyrins / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D019743:Chemokines, CXC; D018840:HSP70 Heat-Shock Proteins; D036341:Intercellular Signaling Peptides and Proteins; D011524:Protoporphyrins; D017392:Thiobarbituric Acid Reactive Substances; C017803:zinc protoporphyrin; D009195:Peroxidase; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D051550:I-kappa B Kinase