Prostate cell lines secrete IL-8 in response to Mycoplasma hominis through Toll-like receptor 2-mediated mechanism.
BACKGROUND
The roles of Toll-like receptor 2 (TLR2) and Mycoplasma in prostate inflammation remain unclear. We investigated whether Mycoplasma induced inflammatory cytokine secretion through TLR2-mediated mechanism in prostate cancer cell line.


METHODS
Cell surface expression of TLR2 on PC-3 cells were examined by flowcytometry. PC-3 cells were stimulated with Mycoplasma hominis (M. hominis), and IL-8 secretion and NF-kappaB activation were examined.


RESULTS
PC-3 cells expressed TLR2 mRNA and cell surface TLR2 protein. The membrane fraction of M. hominis induced IL-8 secretion from PC-3 cells and NF-kappaB activation in a concentration-dependent manner. Transient transfection of the dominant negative mutant TLR2(P681H) into PC-3 cells attenuated M. hominis-induced IL-8 secretion and NF-kappaB activation. Antibody against the extracellular TLR2 domain significantly suppressed M. homnis-induced IL-8 secretion from the prostate cell lines including PC-3, PrEC, and transformed myofibroblasts.


CONCLUSIONS
These results clearly demonstrate that the prostate cell line can secrete inflammatory cytokine in response to M. hominis through a TLR2-mediated mechanism.
Takeyama
Koh
K
Department of Biochemistry, Sapporo Medical University School of Medicine, Sapporo, Japan.
Mitsuzawa
Hiroaki
H
Shimizu
Takeyuki
T
Konishi
Masanori
M
Nishitani
Chiaki
C
Sano
Hitomi
H
Kunishima
Yasuharu
Y
Matsukawa
Masanori
M
Takahashi
Satoshi
S
Shibata
Ken-Ichiro
K
Tsukamoto
Taiji
T
Kuroki
Yoshio
Y
D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D019535:Mycoplasma hominis / Q000472:pathogenicity*; D016328:NF-kappa B / Q000502:physiology; D011467:Prostate / Q000166:cytology* / Q000473:pathology*; D051195:Toll-Like Receptor 2 / Q000502:physiology*
D016209:Interleukin-8; D016328:NF-kappa B; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2