Purification and characterization of aldoxime dehydratase of the head blight fungus, Fusarium graminearum.
Fungal aldoxime dehydratase (Oxd) of Fusarium graminearum MAFF305135 was purified and characterized for the first time from its overexpressing Escherichia coli transformant. The enzyme showed about 20% identity with known Oxds, and had similar enzymatic properties with nitrilase-linked Oxd from the Bacillus strain. It belongs to a group of phenylacetaldoxime dehydratases (EC 4.99.1.7), based on its substrate specificity and kinetic analysis.
Kato
Yasuo
Y
Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Japan.
Asano
Yasuhisa
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D005670:Fusarium / Q000201:enzymology*; D006836:Hydro-Lyases / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008190:Lyases; D017385:Sequence Homology; D013379:Substrate Specificity
D001426:Bacterial Proteins; D008190:Lyases; D006836:Hydro-Lyases; C412124:aldoxime dehydratase; C404242:oxd protein, Bacillus