title: Tat(28-35)SL8-specific CD8+ T lymphocytes are more effective than Gag(181-189)CM9-specific CD8+ T lymphocytes at suppressing simian immunodeficiency virus replication in a functional in vitro assay.
abstract: Epitope-specific CD8+ T lymphocytes may play an important role in controlling human immunodeficiency virus (HIV)/simian immunodeficiency virus replication. Unfortunately, standard cellular assays do not measure the antiviral efficacy (the ability to suppress virus replication) of CD8+ T lymphocytes. Certain epitope-specific CD8+ T lymphocytes may be better than others at suppressing viral replication. We compared the antiviral efficacy of two immunodominant CD8+ T lymphocyte responses--Tat(28-35)SL8 and Gag(181-189)CM9--by using a functional in vitro assay. Viral suppression by Tat-specific CD8+ T lymphocytes was consistently greater than that of Gag-specific CD8+ T lymphocytes. Such differences in antigen-specific CD8+-T-lymphocyte efficacy may be important for selecting CD8+ T lymphocyte epitopes for inclusion in future HIV vaccines.
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715, USA
authors.forename: John T
authors.initials: JT
authors.lastname: Loffredo
authors.forename: Eva G
authors.initials: EG
authors.lastname: Rakasz
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Giraldo
authors.forename: Sean P
authors.initials: SP
authors.lastname: Spencer
authors.forename: Kelly K
authors.initials: KK
authors.lastname: Grafton
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Martin
authors.forename: Gnankang
authors.initials: G
authors.lastname: Napoé
authors.forename: Levi J
authors.initials: LJ
authors.lastname: Yant
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Wilson
authors.forename: David I
authors.initials: DI
authors.lastname: Watkins
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D015683:Gene Products, gag / Q000276:immunology; D015696:Gene Products, tat / Q000276:immunology; D008253:Macaca mulatta; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D014779:Virus Replication / Q000276:immunology* / Q000502:physiology
chemical_list: D000956:Antigens, Viral; D018984:Epitopes, T-Lymphocyte; D015683:Gene Products, gag; D015696:Gene Products, tat