Enhancement of superficial pseudohyphal growth by overexpression of the SFG1 gene in yeast Saccharomyces cerevisiae.
In response to nitrogen limitation, diploid yeast strains of Saccharomyces cerevisiae undergo a dimorphic transition to a filamentous growth form known as pseudohyphal growth. This developmental change can be classified into two distinct growing forms: invasive pseudohyphal growth and superficial pseudohyphal growth. We identified a yeast gene, SFG1, whose overexpression predominantly enhances superficial pseudohyphal growth when starved for nitrogen. Sfg1 has a sequence similarity to members of a family of transcriptional regulators of fungal development. Cells of a homozygous sfg1/sfg1 diploid strain have a serious defect in pseudohyphal growth, indicating that Sfg1 has an essential function for pseudohyphal development. Our analyses show that Sfg1 may act separately from mitogen-activated protein kinase (MAPK) pathway and cAMP-dependent protein kinase A (PKA) pathway.
Fujita
Atsushi
A
Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6-4, 1-1 Higashi, Tsukuba 305-8566, Japan. a-fujita@aist.go.jp
Hiroko
Takatoshi
T
Hiroko
Fumika
F
Oka
Chitoshi
C
D000595:Amino Acid Sequence; D005800:Genes, Fungal; D020935:MAP Kinase Signaling System; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D001616:beta-Galactosidase / Q000235:genetics
D029701:Saccharomyces cerevisiae Proteins; D001616:beta-Galactosidase