title: Sensitive determination of phenolic compounds using high-performance liquid chromatography with cerium(IV)-rhodamine 6G-phenolic compound chemiluminescence detection.
abstract: A simple, selective and sensitive determination method of 20 phenolic compounds has been developed using high-performance liquid chromatography (HPLC) with chemiluminescence detection. The method is based on the chemiluminescent enhancement by phenolic compound of the cerium(IV)-rhodamine 6G system in sulfuric acid medium. Twenty phenolic compounds were separated on a XDB-C(8) column with a gradient elution using a mixture of methanol and 1.0% acetic acid as a mobile phase. Under the optimized conditions, a linear working range extends 2 orders of magnitude with the relative standard deviations of intra- and inter-day precision below 4.0%, and the detection limits (S/N = 3) were in the range of 1.5-82.1 ng/ml. The chemiluminescence reaction was compatible with the mobile phase of high-performance liquid chromatography. The proposed method has been successfully applied to the assay of phenolic compounds in red wine without any pretreatment.
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Hefei, Anhui.
authors.forename: Qunlin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Cui
authors.forename: Aung
authors.initials: A
authors.lastname: Myint
authors.forename: Mei
authors.initials: M
authors.lastname: Lian
authors.forename: Lijuan
authors.initials: L
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods; D009942:Organometallic Compounds / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D012235:Rhodamines / Q000737:chemistry*; D014920:Wine / Q000032:analysis
chemical_list: D009942:Organometallic Compounds; D010636:Phenols; D012235:Rhodamines; C506935:cerium(IV)-rhodamine 6G