Structure and chromosomal DNA binding of the SWIRM domain.
The evolutionarily conserved Swi3p, Rsc8p and Moira (SWIRM) domain is found in many chromosomal proteins involved in chromatin modifications or remodeling. Here we report the three-dimensional solution structure of the SWIRM domain from the human transcriptional adaptor ADA2alpha. The structure reveals a five-helix bundle consisting of two helix-turn-helix motifs connected by a central long helix, reminiscent of the histone fold. Using structural and biochemical analyses, we showed that the SWIRM domains of human ADA2alpha and SMARC2 bind to double-stranded and nucleosomal DNA, and we identified amino acid residues required for this function. We demonstrated that the ADA2alpha SWIRM domain is colocalized with lysine-acetylated histone H3 in the cell nucleus and that it potentiates the ACF remodeling activity by enhancing accessibility of nucleosomal linker DNA bound to histone H1. These data suggest a functional role of the SWIRM domain in chromatin remodeling.
Qian
Chengmin
C
Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York University, One Gustave L. Levy Place, New York, New York 10029, USA.
Zhang
Qiang
Q
Li
SiDe
S
Zeng
Lei
L
Walsh
Martin J
MJ
Zhou
Ming-Ming
MM
D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000737:chemistry; D002843:Chromatin / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D002875:Chromosomes / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins; D019077:Helix-Turn-Helix Motifs; D006657:Histones / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000378:metabolism; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D002843:Chromatin; D004268:DNA-Binding Proteins; D006657:Histones; D009707:Nucleosomes; C097695:TADA2A protein, human; D014157:Transcription Factors; D004247:DNA