[Study on breeding up high-yield strain of taxol by protoplast mutagensis].
In order to obtain resistant mutants to nystatin, ultraviolet radiation and LiCl were used to mutagenize the protoplasts of taxol-producing fungi NCEU-1, and four positive mutants with high yield of taxol were screened out on nystatin flat. After further screening experiments on fermentation, a mutant strain--UL04-5 which was able to produce taxol with high yield and could be stably passed on in genetics was eventually found, it's ability to produce taxol was improved from 314.07 microg/L (strain NCEU-1) to 418.24 microg/L (strain U04-5).
Zhao
Kai
K
Life and Science College of Heilongjiang University, Harbin 150080, China.
Zhou
Dong-Po
DP
Ping
Wen-Xiang
WX
Liu
Jun
J
Ma
Xi
X
Jin
Tao
T
D001203:Ascomycota / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D014644:Genetic Variation; D016296:Mutagenesis; D009761:Nystatin / Q000494:pharmacology; D017239:Paclitaxel / Q000096:biosynthesis*; D011523:Protoplasts / Q000378:metabolism*
D009761:Nystatin; D017239:Paclitaxel