Efficient primer strand extension beyond oxadiazole carboxamide nucleobases.
Two oxadiazole carboxamide deoxyribonucleoside analogues are described that can be incorporated and efficiently extended by Taq DNA polymerase. The primer strand extension beyond oxadiazole nucleoside analogues occurs at rates similar to the values observed for the canonical Watson-Crick base pairs irrespective of the template nucleobase. These distinctive chemical effects in DNA polymerase extensions are attributed to the smaller size and unique electronic properties of the oxadiazole nucleobase.
Adelfinskaya
Olga
O
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA.
Nashine
Vishal C
VC
Bergstrom
Donald E
DE
Davisson
V Jo
VJ
D000225:Adenine / Q000737:chemistry*; D003596:Cytosine / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry*; D006147:Guanine / Q000737:chemistry*; D015394:Molecular Structure; D010069:Oxadiazoles / Q000737:chemistry*; D013941:Thymine / Q000737:chemistry*
D017931:DNA Primers; D010069:Oxadiazoles; D006147:Guanine; D003596:Cytosine; D000225:Adenine; D013941:Thymine