title: Characterization of high molecular weight impurities in synthetic phosphorothioate oligonucleotides.
abstract: Phosphorothioate oligonucleotides manufactured by standard phosphoramidite techniques using 2'-deoxyadenosine- or 2'-O-(2-methoxyethyl)-5-methylcytosine-loaded solid supports contain branched impurities consisting of two chains linked through the exocyclic amino group of the 3'-terminal nucleoside of one chain and the 3'-terminal hydroxyl group of another via a P(O)SH group. These impurities are not produced when a universal, non-nucleoside derivatized support is used.
authors.affiliation: Isis Pharmaceuticals Inc., 1896 Rutherford Road, Carlsbad, CA 92008, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Kurata
authors.forename: Kym
authors.initials: K
authors.lastname: Bradley
authors.forename: Hans
authors.initials: H
authors.lastname: Gaus
authors.forename: Nhuy
authors.initials: N
authors.lastname: Luu
authors.forename: Isaiah
authors.initials: I
authors.lastname: Cedillo
authors.forename: Vasulinga T
authors.initials: VT
authors.lastname: Ravikumar
authors.forename: Kent
authors.initials: K
authors.lastname: Van Sooy
authors.forename: James V
authors.initials: JV
authors.lastname: McArdle
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Capaldi
mesh_terms: D044503:5-Methylcytosine / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003839:Deoxyadenosines / Q000737:chemistry; D015394:Molecular Structure; D008970:Molecular Weight; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013873:Thionucleotides / Q000737:chemistry*
chemical_list: D003839:Deoxyadenosines; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D013873:Thionucleotides; C434331:phosphoramidite; D044503:5-Methylcytosine