G-CSF protects human cerebral hybrid neurons against in vitro ischemia.
Granulocyte colony-stimulating factor (G-CSF) protects neurons against experimental focal cerebral ischemia. However, its neuroprotective effect on human brain is unknown. We sought to determine whether G-CSF can protect the human cerebral neurons in vitro. Human cerebral-neuroblastoma hybrid cell line (A1) was exposed to oxygen and glucose deprivation with or without G-CSF. G-CSF promoted cell survival and decreased cytotoxicity effectively at 25 ng/ml. G-CSF reduced early apoptotic (annexin V+/PI-), and late apoptotic or necrotic (annexin V+/PI+) cells, and decreased active caspase-3 immunoreactivity. G-CSF could protect human cerebral neurons following in vitro ischemia.
Jung
Keun-Hwa
KH
Stroke & Neural Stem Cell Laboratory in Clinical Research Institute, Department of Neurology, Seoul National University Hospital, Seoul, South Korea.
Chu
Kon
K
Lee
Soon-Tae
ST
Kang
Lami
L
Kim
Seung U
SU
Kim
Manho
M
Roh
Jae-Kyu
JK
D015153:Blotting, Western; D002545:Brain Ischemia / Q000473:pathology*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D006822:Hybrid Cells / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009336:Necrosis; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D014158:Transcription, Genetic / Q000187:drug effects
D018696:Neuroprotective Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D016179:Granulocyte Colony-Stimulating Factor; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue