Development and application of a sol-gel immunosorbent-based method for the determination of isoproturon in surface water.
An immunosorbent was fabricated by encapsulation of monoclonal anti-isoproturon antibodies in sol-gel matrix. The immunosorbent-based loading, rinsing and eluting processes were optimized. Based on these optimizations, the sol-gel immunosorbent (SG-IS) selectively extracted isoproturon from an artificial mixture of 68 pesticides. In addition to this high selectivity, the SG-IS proved to be reusable. The SG-IS was combined with liquid chromatography-tandem mass spectrometry (LC-MS-MS) to determine isoproturon in surface water, and the linear range was up to 2.2 microg/l with correlation coefficient higher than 0.99 and relative standard deviation (RSD) lower than 5% (n=8). The limit of quantitation (LOQ) for 25-ml surface water sample was 5 ng/l.
Zhang
Xiuli
X
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
Martens
Dieter
D
Krämer
Petra M
PM
Kettrup
Antonius A
AA
Liang
Xinmiao
X
D002138:Calibration; D005782:Gels; D006540:Herbicides / Q000032:analysis*; D010671:Phenylurea Compounds / Q000032:analysis*; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis*
D005782:Gels; D006540:Herbicides; D010671:Phenylurea Compounds; D014874:Water Pollutants, Chemical; C028904:isoproturon