title: Evaluation of energy state of islet independent of size using a newly developed ATP bioluminescence assay.
abstract: ATP content or energy charge (EC) of islets may be a good parameter to assess viability. In this study, we examined adenine nucleotides and EC of freshly isolated or 24-hour cultured rat islets of various diameters using a novel bioluminescent enzymatic cycling assay system. For freshly isolated islets, ATP content and islet diameter showed a high correlation (r = 0.842, P < .001), but a significant correlation was not observed for cultured islets (r = 0.284) when all islets were included for the analysis. When only the cultured islets with a diameter <350 microm were included for analysis, a significant correlation was observed (r = 0.719). EC of freshly isolated islets fluctuated widely irrespective of diameter, in contrast with results of 24-hour cultured islets, which showed a stable, high EC, regardless of diameter. These data suggest that the ATP content of islets correlates with the islet size and that EC of islets widely fluctuates following isolation, indicating a significant role of monitoring ATP and EC of islets before transplantation.
authors.affiliation: Department of Surgery I, Fukushima Medical University, 1 Hikarigaoka, Fukushima 960-1295, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ishii
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Ise
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Tsutiya
authors.forename: A
authors.initials: A
authors.lastname: Kenjo
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Anazawa
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Terashima
authors.forename: M
authors.initials: M
authors.lastname: Gotoh
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques; D048429:Cell Size; D004734:Energy Metabolism / Q000502:physiology*; D007515:Islets of Langerhans / Q000166:cytology / Q000502:physiology*; D008163:Luminescent Measurements; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000255:Adenosine Triphosphate