Changes in the localization of antigen presenting cells and T cells in the utero-vaginal junction after repeated artificial insemination in laying hens.
The goal of our present study was to observe whether the populations of antigen presenting cells (Ia+ cells) and T cell subsets (CD4+ and CD8+ T cells) change in the utero-vaginal junction (UVJ) of Rhode Island Red laying hens that showed dramatic declines in fertility after repeated artificial insemination (AI). Rhode Island Red laying hens were divided into two groups: a virgin group (R-V) and artificial inseminated group (R-AI), which was exposed to weekly AI for a period of 3 mo. Undiluted fresh semen collected from healthy Tosa-Jidori roosters, a native Japanese breed maintained in Kochi Prefecture, was used for AI. The UVJ tissues were processed for frozen sections, and Ia+ cells and CD4+ and CD8+ T cells were identified by immunohistochemistry. The Ia+ cells and CD4+ and CD8+ T cells were observed in the stroma and mucosal epithelium of UVJ in both the R-AI and R-V birds. The frequencies of them in the stroma were significantly higher in R-AI than R-V. The higher frequency of Ia+ cells in the UVJ of R-AI group indicated a greater potential capability for antigen presentation to CD4+ cells. The significant increase in CD8+ and CD4+ T cells in the UVJ of R-AI birds might be the result of a homing process of lymphocytes, which may affect sperm survivability and fertility.
Das
Shubash Chandra
SC
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
Nagasaka
Naohiro
N
Yoshimura
Yukinori
Y
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D005260:Female; D005298:Fertility / Q000276:immunology / Q000502:physiology; D007150:Immunohistochemistry / Q000662:veterinary; D007315:Insemination, Artificial / Q000009:adverse effects / Q000276:immunology* / Q000662:veterinary; D008297:Male; D052287:Oviparity; D010058:Oviposition; D014599:Uterus / Q000276:immunology*; D014621:Vagina / Q000276:immunology*