title: Full assignment of the 1H and 13C spectra and revision of the O-acetylation site of the capsular polysaccharide of Streptococcus pneumoniae Type 33F, a component of the current pneumococcal polysaccharide vaccine.
abstract: The structure of the capsular polysaccharide from Streptococcus pneumoniae Type 33F was originally determined by a combination of chemical methods and limited use of NMR spectroscopy [Can. J. Biochem. Cell Biol.1984, 62, 666-677]. We report full 1H and 13C assignments and confirm the structure of the saccharide repeat unit, but find that the site of O-acetylation is O-2 of the -->5)-beta-D-Galf, rather than the -->3)-beta-D-Galf residue. We find that a slightly higher percentage of the repeat units are O-acetylated: [carbohydrate: see text].
authors.affiliation: Laboratory for Molecular Structure, National Institute for Biological Standards and Control Blanche Lane, South Mimms, Herts EN6 3QG, UK.
authors.forename: Xavier
authors.initials: X
authors.lastname: Lemercinier
authors.forename: Christopher
authors.initials: C
authors.lastname: Jones
mesh_terms: D000107:Acetylation; D016667:Bacterial Capsules; D002240:Carbohydrate Sequence; D004578:Electron Spin Resonance Spectroscopy; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D022242:Pneumococcal Vaccines / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013296:Streptococcus pneumoniae / Q000737:chemistry
chemical_list: D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial; C116872:capsular polysaccharide 33F