[Sequence analysis of the mutated genes in CTXEVC Phi and nct-CTXnew Phi genomes in Vibrio cholerae JS94484 strain].
OBJECTIVE
To analyze the sequences of the mutated genes in CTX(EVC)Phi and nct-CTX(new)Phi genomes in Vibrio cholerae JS94484 strain.


METHODS
The mutated genes in CTX(EVC)Phi and nct-CTX(new)Phi genome were obtained by PCR, sequenced and analyzed.


RESULTS
ig1, rstR, ig2, and ctxAB genes in CTX(EVC)Phi genome of V. cholerae strain JS94484 were highly homologous with those of standard EVC strain N16961, while ig1, rstR and ig2 genes in nct-CTX(new)Phi genome of the strain JS94484 shared low homology with those of the other 3 biotypes of V. cholerae. Considerable difference was detected in the last 60 bp of zot genes between CTX(EVC)Phi and nct-CTX(new)Phi genomes, indicating possible difference in the amino sequences of the Zot proteins encoded by these two genes. The sequence of toxin-coregulated pilus A subunit gene (tcpA) of the strain JS94484 was identical with that of strain N16961.


CONCLUSIONS
ig1, rstR and ig2 genes of nct-CTX(new)Phigenome are of a novel type, and their functions await further investigation.
Rui
Yong-yu
YY
Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. yongyurui@tom.com
Kan
Biao
B
Gao
Shou-yi
SY
Liu
Yan-qing
YQ
Qi
Guo-ming
GM
D002772:Cholera Toxin / Q000235:genetics; D003001:Cloning, Molecular; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D016680:Genome, Bacterial / Q000235:genetics*; D017912:Inovirus / Q000235:genetics; D009154:Mutation; D039002:Prophages / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics*; D041242:Vibrio cholerae O139 / Q000235:genetics / Q000378:metabolism; D016662:Virus Integration
C085807:TcpA protein, Vibrio cholerae; D036781:Fimbriae Proteins; D002772:Cholera Toxin