title: Proteomic characterization of Yersinia pestis virulence.
abstract: The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Caf1), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.
authors.affiliation: Biosciences Directorate, Lawrence Livermore National Laboratory, CA 94550, USA. chromy@llnl.gov
authors.forename: Brett A
authors.initials: BA
authors.lastname: Chromy
authors.forename: Megan W
authors.initials: MW
authors.lastname: Choi
authors.forename: Gloria A
authors.initials: GA
authors.lastname: Murphy
authors.forename: Arlene D
authors.initials: AD
authors.lastname: Gonzales
authors.forename: Chris H
authors.initials: CH
authors.lastname: Corzett
authors.forename: Brian C
authors.initials: BC
authors.lastname: Chang
authors.forename: J Patrick
authors.initials: JP
authors.lastname: Fitch
authors.forename: Sandra L
authors.initials: SL
authors.lastname: McCutchen-Maloney
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D002118:Calcium; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D010544:Peroxidases / Q000032:analysis / Q000378:metabolism; D010960:Plasminogen Activators / Q000032:analysis / Q000378:metabolism; D013696:Temperature; D037521:Virulence Factors / Q000032:analysis / Q000378:metabolism; D015010:Yersinia pestis / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D037521:Virulence Factors; C086725:caf1 protein, Yersinia pestis; D010544:Peroxidases; C081610:catalase-peroxidase, bacteria; D010960:Plasminogen Activators; D002118:Calcium