title: A prospective follow-up of Epstein-Barr virus LMP1 genotypes in saliva and blood during infectious mononucleosis.
abstract: To monitor multiple Epstein-Barr virus (EBV) infections during the early and convalescent stages of infectious mononucleosis (IM), a cloning and sequencing study of the LMP1 gene was conducted in saliva and peripheral blood mononuclear cells (PBMCs) from 23 patients with IM at day 0 (D0) and day 180 (D180) after the onset of the disease. Multiple EBV strains were detected in 9 (39%) of the patients during follow-up, with 7 of 9 cases detected as early as D0. Six of the nine patients harbored the same dominant strain in saliva and PBMCs during follow-up, with a trend toward a restriction of the number of EBV strains in saliva but not in PBMCs at D180. Furthermore, transmission of a minor strain was observed between partners in a heterosexual couple. There was no correlation between multiple infections and EBV DNA load in either compartment.
authors.affiliation: Laboratoire de Virologie Moleculaire et Structurale, Universite Joseph Fourier, Centre Hospitalier Universitaire, Grenoble, France.
authors.forename: Samira
authors.initials: S
authors.lastname: Fafi-Kremer
authors.forename: Patrice
authors.initials: P
authors.lastname: Morand
authors.forename: Raphaele
authors.initials: R
authors.lastname: Germi
authors.forename: Mirvat
authors.initials: M
authors.lastname: Ballout
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Brion
authors.forename: Odile
authors.initials: O
authors.lastname: Genoulaz
authors.forename: Sandrine
authors.initials: S
authors.lastname: Nicod
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Stahl
authors.forename: Rob W H
authors.initials: RW
authors.lastname: Ruigrok
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Seigneurin
mesh_terms: D003001:Cloning, Molecular; D005260:Female; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007244:Infectious Mononucleosis / Q000635:transmission / Q000821:virology*; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008297:Male; D012463:Saliva / Q000821:virology*; D017422:Sequence Analysis, DNA; D019562:Viral Load; D014763:Viral Matrix Proteins / Q000235:genetics
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins