title: Identification of an HLA-A24-restricted OY-TES-1 epitope recognized by cytotoxic T-cells.
abstract: OY-TES-1 was identified as a human homologue of the mouse, guinea pig, and pig proacrosin binding protein sp32 precursor. Differential expression levels of OY-TES-1 mRNA between testis and other normal tissues, and its expression in cancers indicated that OY-TES-1 would be classified as a cancer/testis antigen and considered to be a candidate of target antigen for cancer immunotherapy. In this study, we showed identification of HLA-A24-binding OY-TES-1 peptide, TES(401-409) (KTPFVSPLL) recognized by CD8 T-cells. Purified CD8 T-cells from healthy donors stimulated in vitro with the peptide-pulsed autologous DC and PBMC produced IFNgamma in response to the peptide-pulsed PBMC and showed cytotoxicity against the peptide-pulsed autologous EBV-B specifically. Furthermore, cytotoxicity was also observed against an OY-TES-1 mRNA-expressing tumor line, LK79. The retention time of the fraction in HPLC of the acid eluate from LK79 cells that showed positive sensitization against autologous EBV-B cells in recognition by CD8 CTL was the same as that of the fraction of the TES(401-409) peptide itself, suggesting that the TES(401-409) was a naturally processed peptide on LK79.
authors.affiliation: Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Okumura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Noguchi
authors.forename: Akiko
authors.initials: A
authors.lastname: Uenaka
authors.forename: Toshiki
authors.initials: T
authors.lastname: Aji
authors.forename: Toshiro
authors.initials: T
authors.lastname: Ono
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Motoi
authors.initials: M
authors.lastname: Aoe
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Shimizu
authors.forename: Eiichi
authors.initials: E
authors.lastname: Nakayama
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology*; D045744:Cell Line, Tumor; D000939:Epitopes; D015234:HLA-A Antigens / Q000378:metabolism*; D059871:HLA-A24 Antigen; D006801:Humans; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: C485651:ACRBP protein, human; D000951:Antigens, Neoplasm; D002352:Carrier Proteins; D000939:Epitopes; D015234:HLA-A Antigens; D059871:HLA-A24 Antigen; D009842:Oligopeptides