title: Short amplicon STR multiplex for stain typing.
abstract: We developed a short tandem repeat (STR) typing kit based on DNA database systems that are included in, for example, the Interpol Standard Set of Loci recommendations (i.e., TH01, VWA, D3S1358, FGA) and the gender typing system Amelogenin. Two different multiplex sets were tested using the fluorescent dyes FAM, JOE, and VIC. The PCR results were compared to the commercially available AmpFISTR Blue kit, which contains the STRs D3S1358, VWA, and FGA. The advantage of our multiplex compared with the Blue kit was the generation of shorter amplicons (<200 bp) and the higher combined power of discrimination.
authors.affiliation: Department of Legal Medicine, University Hospital, Ulm, Germany. peter.wiegand@medizin.uni-ulm.de
authors.forename: P
authors.initials: P
authors.lastname: Wiegand
authors.forename: R
authors.initials: R
authors.lastname: Klein
authors.forename: G
authors.initials: G
authors.lastname: Braunschweiger
authors.forename: C
authors.initials: C
authors.lastname: Hohoff
authors.forename: B
authors.initials: B
authors.lastname: Brinkmann
mesh_terms: D053523:Amelogenin / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005456:Fluorescent Dyes; D006801:Humans; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
chemical_list: D053523:Amelogenin; D017931:DNA Primers; D005456:Fluorescent Dyes