[Identification, cloning and sequencing of GST gene of bacterium degrading poly-aromatic hydrocarbons].
A bacterium (designated strain ZX4) able to degrade poly aromatic hydrocarbons was isolated from oil-contaminated soil. Based on analysis of the 16S rDNA sequence, whole cell fatty acid, and Biolog-GN, the strain was identified as Sphingomonas paucimobilis. The detection of GST (Glutathione S-transferase) indicated that the GST from the strain had the activity of conjugating with CDNB and it maybe relate with degradation of PAHs. The PCR product of GST gene from the strain confirmed its presence. Phylogeny analysis based on gst sequence indicated that the strain was relatively close with the strain which can degrade PAH likewise.
Xia
Ying
Y
College of Life Sciences, Zhejiang University, Hangzhou 310029, China. xiaying7702@sohu.com
Min
Hang
H
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D020578:Sphingomonas / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006841:Hydrocarbons, Aromatic; D012336:RNA, Ribosomal, 16S; D005982:Glutathione Transferase