A PNA-DNA hybridization chip approach for the detection of beta-secretase activity.
Developed was the addressable chip technology based on the PNA-DNA complementary hybridization equipped with short seven-mer PNA-encoded peptides that can be a versatile scaffold to monitor on-chip immunoassays. We also developed and validated a methodology to perform beta-secretase enzyme assay with a highly sensitive fashion, resulting that a peptide substrate tethering dual fluorescent probes allowed us to detect beta-secretase activity 10 times more sensitively than assays in solution.
Sano
Shusuke
S
Department of Bioengineering and The COE 21 Program, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, B-40, 4259 Nagatsuta, Midori, Yokohama 226-8501, Japan.
Tomizaki
Kin-ya
KY
Usui
Kenji
K
Mihara
Hisakazu
H
D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; D001483:Base Sequence; D010450:Endopeptidases / Q000032:analysis*; D006801:Humans; D007118:Immunoassay; D015335:Molecular Probes / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020135:Peptide Nucleic Acids / Q000032:analysis* / Q000235:genetics
D015335:Molecular Probes; D020135:Peptide Nucleic Acids; D053829:Amyloid Precursor Protein Secretases; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; C509280:BACE1 protein, human