A prospective follow-up of Epstein-Barr virus LMP1 genotypes in saliva and blood during infectious mononucleosis.
To monitor multiple Epstein-Barr virus (EBV) infections during the early and convalescent stages of infectious mononucleosis (IM), a cloning and sequencing study of the LMP1 gene was conducted in saliva and peripheral blood mononuclear cells (PBMCs) from 23 patients with IM at day 0 (D0) and day 180 (D180) after the onset of the disease. Multiple EBV strains were detected in 9 (39%) of the patients during follow-up, with 7 of 9 cases detected as early as D0. Six of the nine patients harbored the same dominant strain in saliva and PBMCs during follow-up, with a trend toward a restriction of the number of EBV strains in saliva but not in PBMCs at D180. Furthermore, transmission of a minor strain was observed between partners in a heterosexual couple. There was no correlation between multiple infections and EBV DNA load in either compartment.
Fafi-Kremer
Samira
S
Laboratoire de Virologie Moleculaire et Structurale, Universite Joseph Fourier, Centre Hospitalier Universitaire, Grenoble, France.
Morand
Patrice
P
Germi
Raphaele
R
Ballout
Mirvat
M
Brion
Jean-Paul
JP
Genoulaz
Odile
O
Nicod
Sandrine
S
Stahl
Jean-Paul
JP
Ruigrok
Rob W H
RW
Seigneurin
Jean-Marie
JM
D003001:Cloning, Molecular; D005260:Female; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007244:Infectious Mononucleosis / Q000635:transmission / Q000821:virology*; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008297:Male; D012463:Saliva / Q000821:virology*; D017422:Sequence Analysis, DNA; D019562:Viral Load; D014763:Viral Matrix Proteins / Q000235:genetics
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins