title: The secretome of Pleurotus sapidus.
abstract: Due to their unique capability to attack lignified biopolymers, extracellular enzymes of white-rot fungi enjoy an increasing interest in various fields of white biotechnology. The edible fungus Pleurotus sapidus was selected as a model organism for the analysis of the secretome by means of 2-DE. For enzyme production, the fungus was grown in submerged cultures either on peanut shells or on glass wool as a carrier material. Identification of the secreted enzymes was performed by tryptic digestion, ESI-MS/MS ab initio sequencing, and homology searches against public databases. The spectrum of secreted enzymes comprised various types of hydrolases and lignolytic enzymes of the manganese peroxidase/versatile peroxidase family. While peptidases were secreted mainly by the cultures grown on peanut shells, versatile peroxidase type enzymes dominated in the cultures grown on glass wool.
authors.affiliation: Zentrum Angewandte Chemie, Institut für Lebensmittelchemie der Universität Hannover, Wunstorfer Strasse 14, D-30453 Hannover, Germany. zorn@lci.uni-hannover.de
authors.forename: Holger
authors.initials: H
authors.lastname: Zorn
authors.forename: Thilo
authors.initials: T
authors.lastname: Peters
authors.forename: Manfred
authors.initials: M
authors.lastname: Nimtz
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Berger
mesh_terms: D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000378:metabolism*; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D020076:Pleurotus / Q000201:enzymology* / Q000502:physiology; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005656:Fungal Proteins