[Archaeal diversity of Zabuye Lake in Tibet analyzed by culture-independent approach].
With culture-independent approach, microbial DNA was directly extracted from samples of Zabuye saline soda lake. Using the microbial DNA as template, archaeal 16S rDNAs were amplified by PCR. Amplified products were cloned and sequenced. 60 different cloned partial sequences, most of which were related to haloalkaliphilic archaeon, were acquired. In the phylogenetic tree, some clones of Zabuye lake belonged to Genus Natronobacterium, Natrinema, Natronococcus, Natronorubrum, Natronomonas, Halorubrum, Haloterrigena, Halorhabdus in Family Halobacteriaceae. Other clones represent some novel groups. All of them show prolific archaeal diversity of Zabuye lake.
Fan
Huapeng
H
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. fhp_dragon@sina.com
Xue
Yanfen
Y
Zeng
Yan
Y
Zhou
Peijin
P
Ma
Yanhe
Y
D001105:Archaea / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D044822:Biodiversity; D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism*; D014871:Water Microbiology
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride