[Genetic diversity analysis of Chinese stylo anthracnose pathogens using random amplified polymorphic DNA].
Genetic diversity of 43 Colletotrichum gloeosporioides isolates from stylo in China were analyzed using random amplified polymorphic DNA (RAPD) with eight arbitrary 10-base oligonucleotide primers compared with 276 isolates from other countries including two of Colletotrichum cutaturn based on a wide survey and disease sample collection. The results showed a good DNA polymorphism between isolates. The amplified fragments were between 0.3-2.8 kb. Chinese isolates were grouped in clusters II, III and VI with the majority in cluster VI based on six clusters of isolates from South America, the centre of origin of Stylosanthes genus. The genetic variation in the Chinese pathogen population was very limited compared with that in South America, the centre of host-pathogen diversity. The results also showed that a genetic variation in Chinese population of Colletotrichum gloeosporioides existed, though this variation was less significant. Isolates grouped by geographic origin and host species or genotypes indicated isolates from different host species or genotypes had their own parasitic specialization on genetic basis and pathogens from different countries evolved in their own way relatively.
Yi
Kexian
K
Tropical Pasture Research Center, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China. yikexian@21cn.com
Huang
Junsheng
J
Liu
Guodao
G
Pauline
Weeds
W
Sukumar
Charkraborty
C
D002681:China; D020231:Colletotrichum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D007887:Fabaceae / Q000382:microbiology*; D014644:Genetic Variation; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique