Platelet activation markers in patients with venous thromboembolism without predisposing factors.
A constant in vitro hypersensitivity of platelets (adenosine diphosphate) has been suggested as a risk factor for arterial and even venous thrombosis. Our aim was to determine phenotypic and functional alterations of platelets by flow cytometry as potential prothrombotic risk factors in patients with a history of unexplained spontaneous venous thrombosis. Forty-nine patients with a history of spontaneous venous thrombosis and no inherited or acquired thrombophilic risk factors were compared with a reference group of 39 healthy volunteers. Flow cytometry (FACS) was used to analyze the surface expression of CD62 (P-selectin) and CD63 in nonactivated platelets and after in vitro stimulation with adenosine diphosphate and thrombin receptor activator peptide 6. Mean fluorescence intensity of CD62 and CD63 surface expression as well as percentage of CD62 and CD63 positive cells and binding index differed in patients with a history of thrombosis compared with the reference group, but failed to reach statistical significance. Similar results were observed after in vitrostimulation with adenosine diphosphate and thrombin receptor activator peptide 6. In conclusion, the expression of CD62 and CD63 of resting and in vitro activated platelets could not be established as a risk factor for spontaneous venous thromboembolism.
Gerdsen
Frank
F
Vascular Centre Berlin, Department of Internal Medicine, Evangelisches Krankenhaus Konigin Elisabeth Herzberge, Berlin, Germany. fgerdsen@hotmail.com
Weber
Martin
M
Langer
Florian
F
Eifrig
Barbara
B
Lindhoff-Last
Edelgard
E
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000096:biosynthesis / Q000097:blood*; D015539:Platelet Activation / Q000187:drug effects; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis; D018179:Receptors, Thrombin / Q000378:metabolism; D012307:Risk Factors; D060149:Tetraspanin 30; D013923:Thromboembolism / Q000097:blood*
D015703:Antigens, CD; D015415:Biomarkers; C554586:CD63 protein, human; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D018179:Receptors, Thrombin; D060149:Tetraspanin 30; D000244:Adenosine Diphosphate