Proteomic characterization of Yersinia pestis virulence.
The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Caf1), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.
Chromy
Brett A
BA
Biosciences Directorate, Lawrence Livermore National Laboratory, CA 94550, USA. chromy@llnl.gov
Choi
Megan W
MW
Murphy
Gloria A
GA
Gonzales
Arlene D
AD
Corzett
Chris H
CH
Chang
Brian C
BC
Fitch
J Patrick
JP
McCutchen-Maloney
Sandra L
SL
D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D002118:Calcium; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D010544:Peroxidases / Q000032:analysis / Q000378:metabolism; D010960:Plasminogen Activators / Q000032:analysis / Q000378:metabolism; D013696:Temperature; D037521:Virulence Factors / Q000032:analysis / Q000378:metabolism; D015010:Yersinia pestis / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity
D001426:Bacterial Proteins; D003470:Culture Media; D037521:Virulence Factors; C086725:caf1 protein, Yersinia pestis; D010544:Peroxidases; C081610:catalase-peroxidase, bacteria; D010960:Plasminogen Activators; D002118:Calcium