Alterations in plasma antioxidants during reperfusion of the ischemic small intestine in rats.
The aim of this study was to evaluate the contribution of three plasma antioxidants (albumin, uric acid, SH groups) to the plasma total peroxyl radical-trapping antioxidant capacity (TRAP) in 2 and 4 h of intestinal reperfusion in rats. TRAP increased significantly both after 2 and 4 h of reperfusion. Neither albumin nor SH groups contributed significantly to this increase. TRAP was strongly influenced by the increase in uric acid concentration and also probably by the cell destruction caused by oxidative stress. Since the TRAP increase was accompanied by an increase in the level of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation), we can conclude that even such a large increase in TRAP is not sufficient to prevent the progression of lipid peroxidation and oxidative stress.
Papezíková
Ivana
I
Laboratory of free radical pathophysiology, Institute of Biophysics, Academy of Sciences of The Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic.
Lojek
Antonín
A
Cízová
Hana
H
Cíz
Milan
M
D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D005260:Female; D007421:Intestine, Small / Q000098:blood supply*; D007511:Ischemia; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D010545:Peroxides / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000503:physiopathology* / Q000662:veterinary; D012709:Serum Albumin; D013438:Sulfhydryl Compounds / Q000097:blood; D014527:Uric Acid / Q000097:blood
D000975:Antioxidants; D010545:Peroxides; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; D014527:Uric Acid; C049375:perhydroxyl radical