Cloning and sequence analysis of myostatin promoter in sheep.
To better understand the structure and function of the myostatin's gene promoter region in sheep, we cloned and sequenced a 1.517 kb fragment containing the 5'-regulatory region of the sheep myostatin gene (GenBank accession number is AY918121). The promoter sequence consists of three TATA boxes, one CAAT box, and eight putative E-boxes. Some putative muscle growth response elements for Octamer-binding factor 1(Octamer), Activator protein 1(AP1), Growth factor independence 1 zinc finger protein (Gfi-1B), Myocyte enhancer factor 2 (MEF2), Muscle-specific Mt binding site (MTBF), Glucocorticoid response elements (GRE) and Progesterone receptor binding site (PRE) were detected. Some of the motifs are conserved as compared to with that in the goat, bovine and porcine myostatin promoters. However, some differences were also found.
Du
Rong
R
Shanxi Agricultural University, College of Animal Science and Technology, Taigu, Shanxi 030801, PR China. durong752@sohu.com
Chen
Yong-Fu
YF
An
Xiao-Rong
XR
Yang
Xing-Yuan
XY
Ma
Yi
Y
Zhang
Lei
L
Yuan
Xiao-Li
XL
Chen
Li-Mei
LM
Qin
Jian
J
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D006041:Goats; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D055435:Myostatin; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000235:genetics*; D013045:Species Specificity; D013552:Swine; D014157:Transcription Factors / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics*
C525911:MSTN protein, human; C525912:Mstn protein, mouse; D055435:Myostatin; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D004247:DNA