title: Fission yeast homologue of Tip41-like proteins regulates type 2A phosphatases and responses to nitrogen sources.
abstract: A fission yeast (Schizosaccharomyces pombe) gene encoding a member of the TIP41-like protein family was identified and characterized. Deletion of the fission yeast tip41 gene leads to slower growth when ammonium chloride is the nitrogen source, but the growth rate is not affected when adenine is the nitrogen source. The tip41 mutant cells also enter the G1 phase of the cell cycle earlier than wild-type cells in response to nitrogen starvation. Overexpression of tip41(+) causes cell death, spherical cell morphology and blocks the shift to G1 phase upon nitrogen starvation. Overexpression of tip41(+) increases the activity of type 2A phosphatase. In a ppa2 deletion strain with reduced PP2A activity, overexpression of tip41(+) no longer blocks the shift to G1 upon nitrogen starvation. These results suggest that fission yeast Tip41 plays a role in cellular responses to nitrogen nutrient conditions at least partly through regulation of type 2A phosphatase activity.
authors.affiliation: Children's Memorial Research Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60614, USA.
authors.forename: Csaba
authors.initials: C
authors.lastname: Fenyvuesvolgyi
authors.forename: Robert T
authors.initials: RT
authors.lastname: Elder
authors.forename: Zsigmond
authors.initials: Z
authors.lastname: Benko
authors.forename: Dong
authors.initials: D
authors.lastname: Liang
authors.forename: Richard Yuqi
authors.initials: RY
authors.lastname: Zhao
mesh_terms: D003001:Cloning, Molecular; D016193:G1 Phase; D005800:Genes, Fungal; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D013045:Species Specificity
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C444301:TIP41 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D009584:Nitrogen