[BabA2, oipA and cagE Helicobacter pylori genotypes in Colombian patients with gastroduodenal diseases].
BACKGROUND
Helicobacter pylori infection is associated with the development of several gastroduodenal diseases. Bacterial virulence genes have been found associated with an increased risk for gastric disease.


OBJECTIVE
Herein, associations were made between the presence of vacA, cagA, cagE, babA2 and oipA genes in H. pylori isolates and the range of clinical consequences of the infection.


METHODS
PCR was used to amplify vacA, cagA, cagE, babA2 and oipA genes in 166 isolates-50 patients with peptic ulcer, 39 with non-atrophic gastritis, 26 with atrophic gastritis, 26 with intestinal metaplasia and 25 with gastric adenocarcinoma.


RESULTS
cagA, cagE, babA2 and oipA genes were found in 73%, 75%, 48% and 74% of isolates, respectively. The cytotoxic vacA s1m1/cagA positive/cage positive genotype was present in 64% (100/157) of isolates. A higher frequency of cytotoxic strains was observed in cancer patients (84%), intestinal metaplasia (91%) and peptic ulcer (81%) in comparison with gastritis patients (50%) (p=0.002, 0.008, 0.007, respectively). The oipA and babA2 frequency was higher in cytotoxic isolates than in non-cytotoxic isolates (oipA: 81% vs. 52%, P=0,003; babA2: 58% vs. 12% (p<0.001). No significant association was found among clinical outcomes and oipA or babA2 genotypes, analyzed alone or in combination with vacA and cagA.


CONCLUSIONS
Therefore, babA2 or oipA genes are not marker indicators of ulcer or cancer.
Quiroga
Andrés Javier
AJ
Laboratorio de Inmunologia, Instituto Nacional de Cancerología, Bogotá, D. C. Colombia.
Cittelly
Diana Marcela
DM
Bravo
María Mercedes
MM
D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003105:Colombia; D005260:Female; D005767:Gastrointestinal Diseases / Q000382:microbiology*; D005798:Genes, Bacterial; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D006801:Humans; D008297:Male; D008875:Middle Aged; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; C400429:BabA protein, Helicobacter pylori; D001426:Bacterial Proteins; D037521:Virulence Factors; C080866:cagA protein, Helicobacter pylori