title: [Expression and clinical significance of cancer-related gene MPS-1 in gastric cancer].
abstract: OBJECTIVE
  To investigate the expression of cancer-related gene MPS-1 in gastric cancer and to evaluate its significance in clinical diagnosis and therapy.
  
  
  METHODS
  The mRNA expression of MPS-1 was determined by polymerase chain reaction after reverse transcription (RT-PCR) in cancer tissues and adjacent non-cancerous tissues from 42 cases with gastric cancer. The expression levels of MPS-1 in 6 gastric cancer cell lines (AGS, MKN-45, SGC 7901, KATO III, N-87 and SNU-1) were also determined by RT-PCR and Western blot.
  
  
  RESULTS
  The MPS-1 mRNA was expressed in all tissues and cell lines. The mRNA expression level of MPS-1 in cancer tissues were 1.37+/- 0.87, significantly higher than 0.99+/- 0.67 in adjacent normal gastric mucous tissues (P< 0.01). The expression of MPS-1 was correlated with TNM stage (P< 0.05), but not with age, gender, tumor size and differentiation. The expression level of MPS-1 mRNA in the primary lesions was hig her in the patients with TNM stages III, IV than those with TNM stages I, II. Meanwhile, RT-PCR and Western blot showed the same results that MPS-1 expression was higher in the six gastric cancer cell lines as compared with that in the normal gastric cell line GES-1.
  
  
  CONCLUSIONS
  The high expression of MPS-1 in gastric cancer indicates that MPS-1 might play an important role in gastric carcinogenesis,which may provide a new target in immunotherapy for gastric cancer.
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai Institute of Digestive Surgery, Shanghai 200025, China.
authors.forename: Yun-wei
authors.initials: YW
authors.lastname: Wang
authors.forename: Zheng-gang
authors.initials: ZG
authors.lastname: Zhu
authors.forename: Bing-ya
authors.initials: BY
authors.lastname: Liu
authors.forename: Qin-long
authors.initials: QL
authors.lastname: Gu
authors.forename: Jian-fang
authors.initials: JF
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Qu
authors.forename: Xue-hua
authors.initials: XH
authors.lastname: Chen
authors.forename: Yan-zhen
authors.initials: YZ
authors.lastname: Lin
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011505:Protein-Tyrosine Kinases; D012334:RNA, Neoplasm / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D018797:Cell Cycle Proteins; D012334:RNA, Neoplasm; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; C075840:TTK protein, human