title: [Relationship between the expression of cyclooxygenase-2 and microvessel density in oral squamnous cell carcinoma].
abstract: OBJECTIVE
  To detective the relationship between cyclooxygenase-2 (COX-2) and angiogenesis in oral squamous cell carcinoma (OSCC) and its clinical significance through observing the expression of COX-2 and determining microvessel density (MVD) in OSCC.
  
  
  METHODS
  PV-9000 immunohistochemistry was used to determine the expression of COX-2 and CD34, which was used to determine MVD, in 76 OSCC tissues and 12 normal oral mucosa tissues.
  
  
  RESULTS
  Overexpression of COX-2 was detected in OSCC, and was more intense compared with normal epithelium (P < 0.001). The high expression of COX-2 in OSCC was related to neck lymphnode metastasis, tumor size, TNM stage and histological grade (P <0.05). The MVD value in COX-2-positive group was much higher than that in COX-2-negative group (P < 0.01) and that in normal oral mucosa tissues (P < 0.01).
  
  
  CONCLUSIONS
  The high expression of COX-2 in OSCC was significantly associated with MVD, neck lymphnode metastasis, tumor size, TNM stage and histological grade. COX-2 might be one of the important factors in the angiogenesis of OSCC.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Xuan-ping
authors.initials: XP
authors.lastname: Cao
authors.forename: Song-tao
authors.initials: ST
authors.lastname: Zhang
authors.forename: Hao-yang
authors.initials: HY
authors.lastname: Wu
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Liu
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Zhang
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell; D051546:Cyclooxygenase 2; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D055806:Microvessels; D008875:Middle Aged; D009061:Mouth Mucosa; D009062:Mouth Neoplasms; D009389:Neovascularization, Pathologic
chemical_list: D051546:Cyclooxygenase 2