Isolation and characterization of a novel platelet aggregation inhibitory peptide from the medicinal mushroom, Inonotus obliquus.
This study describes the extraction and characterization of a platelet aggregation inhibitory peptide from Inonotus obliquus. Ethanol extract from I. obliquus ASI 74006 mycelia showed the highest platelet aggregation inhibitory activity (81.2%). The maximum platelet aggregation inhibitory activity was found when the mycelia of I. obliquus ASI 74006 was extracted with ethanol at 80 degrees C for 12 h. The platelet aggregation inhibitor was purified by systematic solvent fractionation, ultrafiltration, Sephadex G-10 column chromatography, and reverse-phase HPLC. The purified platelet aggregation inhibitor is a novel tripeptide with a molecular mass of 365 Da, having a sequence of Trp-Gly-Cys. The purified platelet aggregation inhibitor also showed high platelet aggregation inhibitory activity in Institute of Cancer Research (ICR) mice.
Hyun
Kwang Wook
KW
Department of Genetic Engineering and Bio-Medicinal Resources Research Center, Paichai University, Daejeon 302-735, Republic of Korea.
Jeong
Seung Chan
SC
Lee
Dae Hyoung
DH
Park
Jeong Sik
JS
Lee
Jong Soo
JS
D000363:Agaricales; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D000431:Ethanol / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010455:Peptides / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D010974:Platelet Aggregation; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D013696:Temperature
D005343:Fibrinolytic Agents; D010455:Peptides; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D000431:Ethanol