Modulation of iodide uptake by dialkyl phthalate plasticisers in FRTL-5 rat thyroid follicular cells.
Plasticisers imparting flexibility to plastics are man-made chemicals abundantly present in the environment. Effects of six different dialkyl phthalates were studied in vitro in the rat thyroid cell line FRTL-5 on their ability to modulate basal iodide uptake mediated by the sodium/iodide symporter (NIS). The present study shows that diisodecyl phthalate (DIDP), dioctyl phthalate (DOP), diisononyl phthalate (DINP) and bis (2-ethylhexyl) phthalate (DEHP) significantly enhance iodide uptake when concentrations in the magnitude between 10(-4) M and 10(-3) M were applied. In this range, these phthalates do not assess toxicity on the cells. Specific inhibiton of NIS demonstrated that enhancement of iodide uptake is due to NIS. In contrast, benzyl butyl phthalate (BBP) also augments iodide uptake at 1mM but this concentration has just exceeded the toxicity threshold and dibutyl phthalate (DBP), the most toxic compound did not modulate iodide uptake at any concentration applied. As we can deduce from our results, plasticisers are capable of significantly modulating NIS mediated iodide uptake activity.
Wenzel
A
A
Department of Internal Medicine I, University of Ulm, Robert-Koch-Strasse 8, Germany.
Franz
C
C
Breous
E
E
Loos
U
U
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D007454:Iodides / Q000378:metabolism*; D010795:Phthalic Acids / Q000494:pharmacology* / Q000633:toxicity; D010968:Plasticizers / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D007454:Iodides; D010795:Phthalic Acids; D010968:Plasticizers; C032279:phthalic acid