Complement C3a and C4a increased in plasma of patients with aspirin-induced asthma.
BACKGROUND
Aspirin-induced asthma (AIA) is a distinct clinical syndrome that affects up to 10% of adults with asthma. Although eicosanoid metabolites appear to play an important role in AIA, the exact pathogenic mechanism for the syndrome remains obscure. In addition, the proposed mechanism fails to explain why aspirin does not cause bronchoconstriction in all individuals.


OBJECTIVE
We aimed to identify proteins that were differentially expressed in between AIA and aspirin-tolerant asthma (ATA) plasma.


RESULTS
By using a proteomics approach, six proteins were found to be differentially expressed in plasma between patients with AIA and patients with ATA at baseline, and eight proteins were significantly up- or down-regulated after aspirin challenge in patients with AIA. These proteins, which were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, can be classified into four groups: complement components, apolipoproteins, modified albumin, and unknown proteins. Among them, the complement component levels in plasma were validated by using ELISA. Plasma concentrations of C3a and C4a were higher in patients with AIA (n = 30) than in patients with ATA (n = 24). After the aspirin challenge, C3 decreased in both patients with AIA and those with ATA, but the C3a concentration increased in the AIA patient group (p = 0.019). Moreover, C3a and C4a levels and the ratios of C3a/C3 and C4a/C4 were correlated with the changes of FEV(1) values after aspirin challenge.


CONCLUSIONS
Aspirin intolerance may be related to alterations in the levels of complements, as well as those of lipoprotein and other proteins.
Lee
Seung-Ha
SH
Genome Research Center for Allergy and Respiratory Diseases, Soonchunhyang University Hospital, Bucheon, Korea.
Rhim
Taiyoun
T
Choi
Yun-Sung
YS
Min
Ji-Won
JW
Kim
Sung-Ho
SH
Cho
Sun-Young
SY
Paik
Young-Ki
YK
Park
Choon-Sik
CS
D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D001241:Aspirin / Q000009:adverse effects*; D001249:Asthma / Q000097:blood* / Q000139:chemically induced*; D015926:Complement C3a / Q000032:analysis*; D015934:Complement C4a / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics
D000894:Anti-Inflammatory Agents, Non-Steroidal; D015926:Complement C3a; D015934:Complement C4a; D001241:Aspirin