Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit.
The key step of N-glycosylation of proteins in the endoplasmic reticulum is catalyzed by the hetero-oligomeric protein complex oligosaccharyltransferase (OST). It transfers the lipid-linked core-oligosaccharide to selected Asn-X-Ser/Thr-sequences of nascent polypeptide chains. Biochemical and genetic approaches have revealed that OST from Saccharomyces cerevisiae consists of nine subunits: Wbp1p, Swp1p, Stt3p, Ost1p, Ost2p, Ost4p, Ost5p, Ostp3 and Ost6p. By blue native polyacrylamide electrophoresis we show that yeast OST consists of two isoforms with distinct functions differing only in the presence of the two related Ost3 and Ost6p proteins. The OST6-complex was found to be important for cell wall integrity and temperature stress. Ost3p and Ost6p are not essential for OST activity, and can in part displace each other in the complex when overexpressed, suggesting a dynamic regulation of the complex formation.
Schwarz
Markus
M
Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany.
Knauer
Roland
R
Lehle
Ludwig
L
D002473:Cell Wall; D004591:Electrophoresis, Polyacrylamide Gel; D006602:Hexosyltransferases / Q000737:chemistry* / Q000502:physiology; D007527:Isoenzymes; D008565:Membrane Proteins / Q000737:chemistry* / Q000502:physiology; D046912:Multiprotein Complexes / Q000737:chemistry / Q000502:physiology; D021122:Protein Subunits; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000502:physiology
D007527:Isoenzymes; D008565:Membrane Proteins; D046912:Multiprotein Complexes; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D006602:Hexosyltransferases; C507060:OST6 protein, S cerevisiae; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase