title: Melanin in the trabecular meshwork is associated with age, POAG but not Latanoprost treatment. A masked morphometric study.
abstract: We wished to conduct a light and electron microscopic investigation of pigmentation within the trabecular meshwork of normals and primary open angle glaucoma (POAG) patients. In particular we wished to get a precise determination of whether there was a relationship between pigmentation and age. In addition we wanted to know if there was a difference between normals and POAGs and whether trabecular meshwork hyperpigmentation was associated with topical latanoprost medication. A total of 25 sham trabeculectomies conducted on post mortem donor eyes provided the age-matched normals and there were 62 trabeculectomy specimens from POAG patients. These were masked and the meshwork subjected to qualitative and quantitative morphological investigation. Light and electron microscopy confirmed that most of the trabecular meshwork melanin was phagocytosed and within meshwork cells. The granules were measured and found to be of the large iris epithelial type. Light microscopic morphometric analysis showed that the number of meshwork cell profiles that contained melanin increased both in normals and POAGs with age. However there was nearly three times more pigmented meshwork cells in the POAGs than the normals. The POAGs were divided into three groups of (1) minimal or no medication prior to surgery, (2) maximal medical therapy and (3) maximum medical therapy including latanoprost (12 specimens). All groups were significantly greater that the normals but of the three it was the maximal medical therapy group (without latanoprost) that had the highest pigmentation. We concluded that pigmentation of the meshwork is age-related and it is elevated in POAG by mechanisms unknown. The melanin accumulation seems to be partly due to the disease process, partly as a consequence of chronic antiglaucoma medication but interestingly not due to latanoprost even in patients where there is iris darkening (four specimens).
authors.affiliation: St Paul's Unit of Ophthalmology, Department of Medicine, Royal Liverpool University Hospital, Liverpool L69 3GA, UK.
authors.forename: Kathryn P B
authors.initials: KP
authors.lastname: Cracknell
authors.forename: Ian
authors.initials: I
authors.lastname: Grierson
authors.forename: Penny
authors.initials: P
authors.lastname: Hogg
authors.forename: Ajesola A
authors.initials: AA
authors.lastname: Majekodunmi
authors.forename: Peter
authors.initials: P
authors.lastname: Watson
authors.forename: Vincent
authors.initials: V
authors.lastname: Marmion
mesh_terms: D000287:Administration, Topical; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D000959:Antihypertensive Agents / Q000008:administration & dosage*; D003593:Cytoplasm / Q000737:chemistry / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism; D005902:Glaucoma, Open-Angle / Q000188:drug therapy / Q000378:metabolism* / Q000601:surgery; D006801:Humans; D007429:Intraocular Pressure / Q000187:drug effects; D000077338:Latanoprost; D008543:Melanins / Q000032:analysis*; D008854:Microscopy, Electron / Q000379:methods; D008875:Middle Aged; D010587:Phagocytosis / Q000502:physiology; D010858:Pigmentation / Q000502:physiology; D011461:Prostaglandins F, Synthetic / Q000008:administration & dosage*; D012189:Retrospective Studies; D014129:Trabecular Meshwork / Q000737:chemistry* / Q000473:pathology; D014130:Trabeculectomy
chemical_list: D000959:Antihypertensive Agents; D008543:Melanins; D011461:Prostaglandins F, Synthetic; D000077338:Latanoprost