[Enhancement of immune responses to hepatitis B DNA vaccine by superantigen SEA in mice].
To investigate the adjuvant effect of plasmid DNA encoding superantigen SEA (D227A) (pmSEA) on immune responses induced by HBV DNA vaccine containing HBV preS2 and S antigen in BABL/c (H-2d). BALB/c mice were immunized intramuscular injection with HBV DNA vaccine (pHBVS2S) mixed with or without pmSEA plasmid. Antibodies againat HBV PreS2 and S antigen in the sera were accessed by Anti-HBs ELISA, and the HBsAg specific cytotoxic T lymphocytes (CTLs) activity was determined by 5 Chromium Release Assay. The HBs peptide-specific IFN-gamma secreting T cells were detected by ELISPOT. Anti-HBs antibody titers and CTLs activity in mice immunized with pmSEA + pHBVS2S group were significant higher (P < 0.05) than pHBVS2S DNA vaccine group. The ratio of IgG1/IgG2a (0.282) was apparently different from the group immunized with peptide (10). Mice immunized with HBV DNA vaccine plus adjuvant produce higher titer of IgG1 and IgG2a antibodies against HBV S antigen 1.36 and 1.73 time higher than that without adjuvant respectively. HBs peptide--specific IFN-gamma secreting T cells increased 2 - 3 times by the pmSEA adjuvant, compared to DNA vaccine group. HBV DNA vaccine (pHBVS2S) induces humoral and cellular immuno-responses in BALB/c mice, and the responses could be significantly boasted by the plasmid encoding mSEA. Therefore the pmSEA was a potential adjuvant for DNA vaccines.
Jin
Yan-Wen
YW
Beijing Institute of Biotechnology, Beijing 100850, China.
Li
Ping
P
Xu
Quan-Bin
QB
Liu
Xuan
X
Huang
Wei
W
Wang
Yun-Long
YL
Cao
Cheng
C
Ma
Qing-Jun
QJ
D000276:Adjuvants, Immunologic; D000818:Animals; D004768:Enterotoxins / Q000276:immunology*; D006509:Hepatitis B / Q000276:immunology / Q000517:prevention & control / Q000628:therapy; D006510:Hepatitis B Antibodies / Q000097:blood*; D017325:Hepatitis B Vaccines / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D013211:Staphylococcus aureus / Q000276:immunology; D018089:Superantigens / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
D000276:Adjuvants, Immunologic; D004768:Enterotoxins; D006510:Hepatitis B Antibodies; D017325:Hepatitis B Vaccines; D018089:Superantigens; D019444:Vaccines, DNA; C026066:enterotoxin A, Staphylococcal; D007371:Interferon-gamma