Detergent-free caveolae proteome suggests an interaction with ER and mitochondria.
Recent proteomic studies of detergent resistant membrane fractions have begun to characterize the protein composition of caveolae and lipid rafts. The methods used in most of these studies, however, are not able to distinguish between plasma membrane and internal membrane lipid domains. Here we used a non-detergent method for obtaining fractions enriched in caveolae derived from the plasma membrane of multiple cell types. Unexpectedly, the proteins in the caveolae proteome suggest these lipid domains may interact with elements of ER and mitochondria. A comparison of the partial proteome we obtained with other published reports identifies 26 proteins that are candidate marker proteins for identifying caveolae in multiple cell types.
McMahon
Kerrie-Ann
KA
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 74390-9039, USA. kerrie-ann.mcmahon@utsouthwestern.edu
Zhu
Meifang
M
Kwon
Sung Won
SW
Liu
Pingsheng
P
Zhao
Yingming
Y
Anderson
Richard G W
RG
D021941:Caveolae / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003902:Detergents / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D008928:Mitochondria / Q000378:metabolism*; D020543:Proteome
D003902:Detergents; D020543:Proteome