title: [Cloning and expression of S9-1 gene of rice black-streaked dwarf virus in Escherichia coli].
abstract: The cDNA of RBSDV S9 was amplified by RT-PCR using the total RNA of infected maize leaves as the template, and then the PCR product was cloned into pBluescriptII SK. After the DNA sequence was determined, the S9-1 gene was subcloned into E. coli expression vector pET-21d. SDS-PAGE analysis revealed that a 40 kD protein was highly expressed. N-terminal sequence analysis confirmed the 40 kD protein was S9-1 protein. Antiserum was prepared using the purified protein. Western blotting indicated that S9-1 protein could be detected in the infected maize leaves.
authors.affiliation: The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Chunbo
authors.initials: C
authors.lastname: Li
authors.forename: Yongwang
authors.initials: Y
authors.lastname: Zhong
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhang
authors.forename: Chunhong
authors.initials: C
authors.lastname: Wei
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008970:Molecular Weight; D010935:Plant Diseases / Q000821:virology; D012087:Reoviridae / Q000235:genetics*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003313:Zea mays / Q000821:virology
chemical_list: D014764:Viral Proteins