[Preparation of chiral alcohol by stereoselective reduction of acetophenone and chloroacetophenone with yeast cells].
Four strains of microorganisms which have activity of chloroacetophenone reduction were screened, in which Saccharomyces cerevisiae B5 showed the highest activity and good stereoselectivity. This strains showed different activity on the reduction of various substrates in the following order: 2'-chloroacetophenone > 2-chloromethylacetophenone > 4'-chloroacetophenone > 3'-chloroacetophenone > acetophenone. Ethanol is the best cosubstrate and its optimal concentration is 5%.
Ou
Zhimin
Z
Institute of Biochemical Engineering, College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China. oozzmm@163.com
Wu
Jianping
J
Yang
Lirong
L
Cen
Peilin
P
D000098:Acetophenones / Q000737:chemistry* / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism; D007218:Industrial Microbiology; D007700:Kinetics; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D015003:Yeasts / Q000737:chemistry / Q000378:metabolism; D002721:omega-Chloroacetophenone / Q000737:chemistry / Q000378:metabolism
D000098:Acetophenones; D000438:Alcohols; D002721:omega-Chloroacetophenone