Microbial modulation in the biomass and toxin production of a red-tide causing alga.
The effect of S10, a strain of marine bacteria isolated from sediment in the Western Xiamen Sea, on the growth and paralytic shellfish poison (PSP) production in the alga Alexandrium tamarense (A. tamarense) was studied under controlled experimental conditions. The results of these experiments have shown that the growth of A. tamarense is obviously inhibited by S10 at high concentrations, however no evident effect on its growth was observed at low concentrations. Its PSP production was also inhibited by S10 at different concentrations, especially at low concentrations. The toxicity of this strain of A. tamarense is about (0.95-12.14) x 10(-6) MU/cell, a peak toxicity value of 12.14 x 10(-6) MU/cell appeared on the 14th day, after which levels decreased gradually. The alga grew well in conditions of pH 6-8 and salinities of 20-34 per thousand. The toxicity of the alga varied markedly at different pH and salinity levels. Toxicity decreased as pH increased, while it increased with salinity and reached a peak value at a salinity of 30 per thousand, after which it declined gradually. S10 at a concentration of 1.02 x 10(9) cells/ml inhibited growth and the PSP production of A. tamarense at different pH and salinity levels. S10 had the strongest inhibitory function on the growth of A. tamarense under conditions of pH 7 and a salinity of 34 per thousand. The best inhibitory effect on PSP production by A. tamarense was at pH 7, this inhibitory effect on PSP production did not relate to salinity. Interactions between marine bacteria and A. tamarense were also investigated using the flow cytometer technique (FCM) as well as direct microscope counting. S10 was identified as being a member of the genus Bacillus, the difference in 16S rDNA between S10 and Bacillus halmapalus was only 2%. The mechanism involved in the inhibition of growth and PSP production of A. tamarense by this strain of marine bacteria, and the prospect of using it and other marine bacteria in the bio-control of red-tides was discussed.
Zheng
T L
TL
School of Life Sciences, Xiamen University, Xiamen 361005, China. wshwzh@jingxian.xmu.edu.cn
Su
J Q
JQ
Maskaoui
K
K
Yu
Z M
ZM
Hu
Z
Z
Xu
J S
JS
Hong
H S
HS
D000818:Animals; D001407:Bacillus / Q000235:genetics / Q000502:physiology*; D015169:Colony Count, Microbial; D017931:DNA Primers; D004141:Dinoflagellida / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008387:Marine Toxins / Q000378:metabolism / Q000633:toxicity*; D051379:Mice; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000032:analysis*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000032:analysis; D018675:Toxicity Tests
D017931:DNA Primers; D008387:Marine Toxins; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride