title: Reconstitution of an apicoplast-localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum.
abstract: In the malaria parasite Plasmodium falciparum isoprenoid precursors are synthesised inside a plastid-like organelle (apicoplast) by the mevalonate independent 1-deoxy-d-xylulose-5-phosphate (DOXP) pathway. The last reaction step of the DOXP pathway is catalysed by the LytB enzyme which contains a [4Fe-4S] cluster. In this study, LytB of P. falciparum was shown to be catalytically active in the presence of an NADPH dependent electron transfer system comprising ferredoxin and ferredoxin-NADP(+) reductase. LytB and ferredoxin were found to form a stable protein complex. These data suggest that the ferredoxin/ferredoxin-NADP(+) reductase redox system serves as the physiological electron donor for LytB in the apicoplast of P. falciparum.
authors.affiliation: Biochemisches Institut, Justus-Liebig-Universität Giessen, Friedrichstrasse 24, 35392 Giessen, Germany.
authors.forename: René C
authors.initials: RC
authors.lastname: Röhrich
authors.forename: Nadine
authors.initials: N
authors.lastname: Englert
authors.forename: Katrin
authors.initials: K
authors.lastname: Troschke
authors.forename: Armin
authors.initials: A
authors.lastname: Reichenberg
authors.forename: Martin
authors.initials: M
authors.lastname: Hintz
authors.forename: Frank
authors.initials: F
authors.lastname: Seeber
authors.forename: Emanuela
authors.initials: E
authors.lastname: Balconi
authors.forename: Alessandro
authors.initials: A
authors.lastname: Aliverti
authors.forename: Giuliana
authors.initials: G
authors.lastname: Zanetti
authors.forename: Uwe
authors.initials: U
authors.lastname: Köhler
authors.forename: Matthias
authors.initials: M
authors.lastname: Pfeiffer
authors.forename: Ewald
authors.initials: E
authors.lastname: Beck
authors.forename: Hassan
authors.initials: H
authors.lastname: Jomaa
authors.forename: Jochen
authors.initials: J
authors.lastname: Wiesner
mesh_terms: D000818:Animals; D002384:Catalysis; D004579:Electron Transport; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010269:Paraquat / Q000737:chemistry; D010963:Plasmodium falciparum / Q000201:enzymology*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics; D013729:Terpenes / Q000378:metabolism*
chemical_list: D005288:Ferredoxins; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D013729:Terpenes; D009249:NADP; D005287:Ferredoxin-NADP Reductase; D010269:Paraquat