[Measuring rupture forces of P-selectin/PSGL-1 bonds using an optical trap assay].
Selectin/ligand interaction plays an important role in such biological processes as inflammatory reaction, tumor metastasis, etc. External forces affect dissociation of receptor-ligand bonds. A novel approach, upon optical trap technique, was developed in this study to investigate the dissociation of P-selectin/PSGL-1 (P-Selectin Glycoprotein Ligand 1) bindings. Stiffness of optical trap was calibrated with laser power using a viscous drag method. While P-selectin and PSGL-1 molecules were functionally coated on surfaces of glass beads, respectively, the dissociation of interacting molecule bond was studied by measuring the rupture force distribution. It was found that most probable rupture force increased with loading rate at < 25 pN/s. These results complemented and validated the current theory at low loading rates.
Zhang
Yan
Y
Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080, China.
Ye
Zhiyi
Z
Huo
Bo
B
Sun
Ganyun
G
Long
Mian
M
D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry*; D055095:Optics and Photonics / Q000295:instrumentation*; D011485:Protein Binding; D017434:Protein Structure, Tertiary
D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein