[Isolation and characterization of a novel radiation-resistant rod-shaped bacterium].
A novel radiation-resistant bacterium was isolated from soil of lake bank in Beijing. The bacterium produced orange-pigmented colonies and formed rod-shaped cells that stained gram negative alike the Deinobacter grandis previously described by Japan's scientist. It was found with electron microscopy that the isolate is of 0.6 microm - 1.6 microm size and has a 30-40 nm thickness of cell wall, being slightly larger and thicker than the Deinobacter grandis. There was a difference in the concentration and molecule weight of catalase between the isolate and the Deincoccus radiodurans R1. The deoxyribonucleic acid guanine plus cytosine (G + C) base ratio was 70.7 mol%. 16S RNA gene sequencing also showed that this rod-shaped bacterium possessed a high homology with the Deinobacter grandis, suggesting that it might be classified into the genus Deinobacter and constitute a new species in this genus.
Lü
Xing
X
Academy of Military Medical Sciences, Institute of Radiation Medicine, Beijing 100850, China. lvx@nic.bmi.ac.cn
Xing
Ruiyun
R
Zhou
Xubin
X
Liu
Qiongming
Q
Zheng
Hui
H
D001426:Bacterial Proteins / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal; D034301:Deinococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000528:radiation effects*; D005618:Fresh Water / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002374:Catalase