How to broaden enzyme substrate specificity: strategies, implications and applications.
For identification of mutations associated with the broadening of enzyme substrate specificity, three strategies, including directed enzyme evolution, are described for selected examples. Implications concerning enzyme models are highlighted. Applications to the field of biocatalysis are discussed. A bidimensional map for the classification of enzyme activities is suggested so as to improve genome annotations.
Jestin
Jean-Luc
JL
Unité de Chimie Organique URA 2128 CNRS, Département de Biologie Structurale et Chimie, Institut Pasteur, 28 Rue du Dr. Roux, 75724 Paris 15, France. jjestin@pasteur.fr
Vichier-Guerre
Sophie
S
D002384:Catalysis; D004253:DNA Nucleotidylexotransferase / Q000235:genetics* / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism*; D019020:Directed Molecular Evolution; D013379:Substrate Specificity; D013824:Thermus / Q000201:enzymology / Q000235:genetics
D004253:DNA Nucleotidylexotransferase; D012321:DNA-Directed RNA Polymerases