title: Substrate phosphorylation affects degradation and interaction to endopeptidase 24.15, neurolysin, and angiotensin-converting enzyme.
abstract: Recent findings from our laboratory suggest that intracellular peptides containing putative post-translational modification sites (i.e., phosphorylation) could regulate specific protein interactions. Here, we extend our previous observations showing that peptide phosphorylation changes the kinetic parameters of structurally related endopeptidase EP24.15 (EC 3.4.24.15), neurolysin (EC 3.4.24.16), and angiotensin-converting enzyme (EC 3.4.15.1). Phosphorylation of peptides that are degraded by these enzymes leads to reduced degradation, whereas phosphorylation of peptides that interacted as competitive inhibitors of these enzymes alters only the K(i)'s. These data suggest that substrate phosphorylation could be one of the mechanisms whereby some intracellular peptides would escape degradation and could be regulating protein interactions within cells.
authors.affiliation: Laboratório de Neurociências, Universidade da Cidade de São Paulo, 03071-000, São Paulo, SP, Brazil.
authors.forename: M F M
authors.initials: MF
authors.lastname: Machado
authors.forename: F M
authors.initials: FM
authors.lastname: Cunha
authors.forename: D A
authors.initials: DA
authors.lastname: Berti
authors.forename: A S
authors.initials: AS
authors.lastname: Heimann
authors.forename: C F
authors.initials: CF
authors.lastname: Klitzke
authors.forename: V
authors.initials: V
authors.lastname: Rioli
authors.forename: V
authors.initials: V
authors.lastname: Oliveira
authors.forename: E S
authors.initials: ES
authors.lastname: Ferro
mesh_terms: D000595:Amino Acid Sequence; D006868:Hydrolysis; D008666:Metalloendopeptidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D010766:Phosphorylation; D013379:Substrate Specificity
chemical_list: D007703:Peptidyl-Dipeptidase A; D008666:Metalloendopeptidases; C022510:thimet oligopeptidase; C062316:neurolysin