[Invasion of Aeromonas hydrophila in cells involving signal transduction and cytoskeleton].
Invasion of Aeromonas hydrophila strain Ah J-1 isolated from diseased fish to cultured HEp-2cells monolayer was evaluated by the recovery of gentamincin-resistant (Gm') bacteria from Triton X-100 cell lysates. The invasive efficiency (IE) could reach to 0.2% at 37 degrees C. In addition, potential signal transduction pathways that precede bacterial internalization were studied by using signal transduction inhibitors. Bacterial internalization of Ah J-1 involved microfilaments and protein tyrosine kinase since cytochalasin D (an inhibitor of microfilament polymerization) and genistein (an inhibitor of protein tyrosine kinase) prevented internalization. Staurosporine (a protein kinase C inhibitor) and Sodium orthovanadate (a protein tyrosine phosphatase inhibitor) accelerated internalization of Ah J-1 into HEp-2 cells.
Chu
Weihua
W
Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. chuweihua2002@yahoo.com.cn
Lu
Chengping
C
D016980:Aeromonas hydrophila / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000378:metabolism*; D005393:Fish Diseases / Q000378:metabolism* / Q000382:microbiology; D005399:Fishes; D016905:Gram-Negative Bacterial Infections / Q000378:metabolism* / Q000382:microbiology / Q000662:veterinary*; D006801:Humans; D015398:Signal Transduction