title: Inhibition of male chick phenotypes and spermatogenesis by Bisphenol-A.
abstract: Bisphenol-A (BPA) has been reported to bind to the estrogen receptor (ER) and also to act as a xenoestrogen on the reproductive system of many species. In our previous study, a high dose of BPA disturbed the growth of the comb and testes of male chickens. In this study, the exposure of relatively low doses of BPA on the growth of the male chicken phenotypes was investigated. White Leghorn male chicks were orally administered various doses of BPA (2 microg to 200 mg/kg) from 2 weeks of age, and thereafter the comb, wattle and testes were examined at 5, 10, 15, 20 and 25 weeks of age. Although the body weight showed no significant difference among the birds of all ages, the growth of above organs was significantly affected in the chicks even with a minimal dose of 2-microg BPA. These inhibitory effects appeared in a dose-dependent manner. Histologically, the growth of the testes was negatively affected by exposure to over 20-microg/kg BPA: namely, the development of seminiferous tubuli and spermatogenesis were severely inhibited. The mRNA expressions of ERalpha and the aromatase gene (p450arom) increased in the testes in a dose-dependent manner after BPA administration. Accordingly, even low doses of BPA delayed the growth of the male chicken phenotype either by a direct effect or by an indirect response resulting in an increase in both of the endogenous estrogen levels and hyper-sensitivity to estrogen.
authors.affiliation: Department of Integrated Structural Biosciences, Veterinary School, Osaka Prefecture University, 1-1 Gakuencho, Sakai, Osaka 599-8531, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Furuya
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Adachi
authors.forename: Sachi
authors.initials: S
authors.lastname: Kuwahara
authors.forename: Kazushige
authors.initials: K
authors.lastname: Ogawa
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tsukamoto
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D002645:Chickens; D003129:Comb and Wattles / Q000187:drug effects* / Q000254:growth & development; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D007150:Immunohistochemistry; D008297:Male; D010636:Phenols / Q000633:toxicity*; D010641:Phenotype; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000473:pathology; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D018809:Proliferating Cell Nuclear Antigen; C006780:bisphenol A; D000098826:Bisphenol A Compounds