title: Gradual graft-cell repopulation with recipient cells following vascularized bone and limb allotransplantation.
abstract: Little is known about the fate of graft cells following vascularized bone allografting. This study was conducted to define the process of graft-cell repopulation with recipient cells. Sixty-five vascularized tibial bone and 50 limb allotransplantations were performed in rat sex-mismatched pairs. FK 506 was used for immunosuppression. The ratio of donor and recipient cells in the graft was evaluated by semiquantitative polymerase chain reaction, using the Y-chromosome primers. Allografted bones had no rejection episodes. In the vascularized bone allograft model, donor-derived cells were gradually replaced by cells of recipient origin, such that by 24 weeks, they comprised only 10% of total cells. In the limb allograft model, male recipient cells were detected in female grafts not at 1 week but at 48 weeks posttransplantation. The ratio of recipient cells was more than 10% in the femur and tibia. Recipient-derived cells gradually migrated into the grafted bone cells with the passage of time.
authors.affiliation: Department of Orthopedic Surgery, Yamaguchi University School of Medicine, Yamaguchi, Japan. muramatu@po.cc.yamaguchiu.ac.jp
authors.forename: Keiichi
authors.initials: K
authors.lastname: Muramatsu
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kurokawa
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Kuriyama
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Taguchi
authors.forename: Allen T
authors.initials: AT
authors.lastname: Bishop
mesh_terms: D000818:Animals; D016025:Bone Transplantation / Q000276:immunology* / Q000473:pathology; D019070:Cell Lineage; D005260:Female; D006614:Hindlimb / Q000637:transplantation*; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D008297:Male; D051381:Rats; D051876:Sex-Determining Region Y Protein / Q000235:genetics; D016559:Tacrolimus / Q000627:therapeutic use; D013977:Tibia / Q000637:transplantation*; D018183:Transplantation Chimera; D014184:Transplantation, Homologous; D014998:Y Chromosome / Q000235:genetics
chemical_list: D007166:Immunosuppressive Agents; D051876:Sex-Determining Region Y Protein; C497747:Sry protein, rat; D016559:Tacrolimus