Analysis of the matrix protein gene sequence of the Asian lineage of peste-des-petits ruminants vaccine virus.
The M gene nucleotide sequence of an Indian peste-des-petits ruminants (PPRV) vaccine virus ("PPRV Sungri/96") belonging to Asian lineage was determined. The gene is 1476 nucleotides long with a single open reading frame (ORF). The nucleotide and predicted amino acid sequence was compared with the homologous region of the African Lineage Vaccine virus "PPRV/Nigeria/75/1". The nucleotide sequence of the "PPRV Sungri/96" was 86% identical to that of "PPRV/Nigeria/75/1", while a homology of 93% and 95% could be observed in the ORF and amino acids level, respectively. The M gene encodes a protein of 335 amino acids, with a predicted molecular weight (MW) of 37.8 kDa. The ORF is flanked by a 3' untranslated region of 436 nucleotides and a high level of sequence divergence (approximately 30%) could be observed in this region between the vaccine viruses of Asian and African lineages. A high degree of conservation of several amino acids of this protein observed previously was also confirmed in this study.
Muthuchelvan
D
D
Central Institute of Fisheries Technology, Cochin 682029, India.
Sanyal
A
A
Sreenivasa
B P
BP
Saravanan
P
P
Dhar
P
P
Singh
R P
RP
Singh
R K
RK
Bandyopadhyay
S K
SK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D007194:India; D008969:Molecular Sequence Data; D029021:Peste-des-Petits-Ruminants / Q000821:virology*; D018107:Peste-des-petits-ruminants virus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014709:Vero Cells; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
D017931:DNA Primers; D004279:DNA, Viral; D014763:Viral Matrix Proteins; D014765:Viral Vaccines