Rapid on-chip postcolumn labeling and high-resolution separations of DNA.
When performing genetic analysis on microfluidic systems, labeling the sample DNA for detection is a critical preparation step. Labeling procedures often involve fluorescently tagged primers and PCRs, which lengthen experimental run times and introduce higher levels of complexity, increasing the overall cost per analysis. Alternatively, on-chip labeling techniques based on intercalating dyes permit rapid labeling of DNA fragments. However, as noted in the literature, the stochastic nature of dye-DNA complex formation hinders the native electrophoretic migration of DNA fragments, degrading the separation resolution. In this study, we present a novel method of controllably labeling DNA fragments at the end of the electrophoretic separation channel in a glass microfluidic chip. Permitting the DNA to separate and labeling just before detection, achieves the rapid labeling associated with intercalators while maintaining the high resolution of native DNA separations. Our analyses are completed in minutes, rather than the hours typical of sample prelabeling. We demonstrate an electrophoretic microchip-based intercalator labeling technique that achieves higher resolution performance than reported in the literature to date.
Sieben
Vincent J
VJ
Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, Canada.
Backhouse
Christopher J
CJ
D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D046209:Electrophoresis, Microchip / Q000379:methods*; D007364:Intercalating Agents / Q000737:chemistry*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods
D007364:Intercalating Agents; D004247:DNA