Effect of nitrogen concentration in culture mediums on growth and enzyme production of Phanerochaete chrysosporium.
Effect of different nitrogen concentration in the mediums on growth and enzyme production of Phanerochaete chrysosporium was studied when glucose concentration was 10 g/L. The results showed that the medium contained 0.8 g/L ammonium tartrate is the best. It not only supply abundant nutrients for the growth of Phanerochaete chrysosporium, which make mycelia the best grow compared with the other medium, but also produce higher manganese-dependent peroxidase (Mnp) and laccase (Lac) activity. In addition, it is observed that the variation of mycelia surface is related to ligninolytic enzyme secreted by Phanerochaete chrysosporium. When the surface of mycelium pellets appeared burs, it predicts secondary metabolism begin. This experimentation demonstrated that when the ratio of carbon and nitrogen in nitrogen limited medium is equal to 100:8, growth and enzyme production of Phanerochaete chrysosporium is the best, it could achieve the maximum Mnp and Lac activity.
Gao
Da-wen
DW
ESPC State Key Joint Laboratory, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China. gdw@tsinghua.edu.cn
Wen
Xiang-hua
XH
Qian
Yi
Y
D018533:Biomass; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000032:analysis*; D042845:Laccase / Q000378:metabolism; D009584:Nitrogen / Q000494:pharmacology*; D010544:Peroxidases / Q000378:metabolism; D020075:Phanerochaete / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development*; D013053:Spectrophotometry; D013644:Tartrates
D003470:Culture Media; D013644:Tartrates; D002244:Carbon; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; D009584:Nitrogen; C029768:tartaric acid