Effect of prostaglandins against alloxan-induced diabetes mellitus.
Previously, we observed that alloxan-induced in vitro cytotoxicity and apoptosis in an insulin secreting rat insulinoma, RIN, cells was prevented by prior exposure to prostaglandin (PG) E(1), PGE(2), PGI(2), PGF(1)(alpha), and PGF(3)(alpha) (P<0.05 compared to alloxan), whereas thromboxane B(2) (TXB(2)) and 6-keto-PGF(1)(alpha) were ineffective. In an extension of these studies, we now report that prior intraperitoneal administration of PGE(1), PGE(2), PGF(1)(alpha), and PGF(3)(alpha) prevented alloxan-induced diabetes mellitus in male Wistar rats, whereas PGI(2), TXB(2), and 6-keto PGF(1)(alpha) were not that effective. PGE(1), PGE(2), PGF(1)(alpha), and PGF(3)(alpha) not only attenuated chemical-induced diabetes mellitus but also restored the antioxidant status to normal range in red blood cells and pancreas. These results suggest that PGE(1), PGE(2), PGF(1)(alpha), and PGF(3)(alpha) can abrogate chemically induced diabetes mellitus in experimental animals and attenuate the oxidant stress that occurs in diabetes mellitus.
Sailaja Devi
M M
MM
UND Life Sciences, Shaker Heights, OH 44120, USA.
Das
U N
UN
D000496:Alloxan / Q000008:administration & dosage / Q000633:toxicity; D000527:Alprostadil / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000032:analysis; D002570:Ceruloplasmin / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000517:prevention & control*; D003922:Diabetes Mellitus, Type 1 / Q000139:chemically induced / Q000517:prevention & control; D015232:Dinoprostone / Q000494:pharmacology; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000032:analysis; D005982:Glutathione Transferase / Q000032:analysis; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000097:blood; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D011453:Prostaglandins / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000032:analysis; D013929:Thromboxane B2 / Q000494:pharmacology
D000975:Antioxidants; D001786:Blood Glucose; D007328:Insulin; D008054:Lipid Peroxides; D011453:Prostaglandins; D011460:Prostaglandins F; D009569:Nitric Oxide; D019344:Lactic Acid; D008315:Malondialdehyde; D013929:Thromboxane B2; D000496:Alloxan; C033246:prostaglandin E3; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D005982:Glutathione Transferase; D000527:Alprostadil; D015232:Dinoprostone; C100006:prostaglandin F1