Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: relevance for natural killer-dendritic cell interaction.
In this study, we demonstrate the involvement of DNAM-1-triggering receptor and its ligands, poliovirus receptor (PVR) and Nectin-2, in natural killer (NK) cell-mediated lysis of dendritic cells (DCs). The surface expression of both ligands was up-regulated in DCs as compared to monocytes. It reached maximal densities after DC maturation induced by different stimuli including lipopolysaccharide (LPS), poly I:C, flagellin, and CD40L. Both immunohistochemical analysis and confocal microscopy revealed expression of DNAM-1 ligands by DCs in lymph nodes in which they were localized in the parafollicular T-cell region and surrounded the high endothelial venules. Remarkably, in cytolytic assays, DNAM-1 cooperated with NKp30 in the NK-mediated killing of both immature and mature DCs and the degree of contribution of DNAM-1 appeared to correlate with the surface densities of its specific ligands PVR and Nectin-2.
Pende
Daniela
D
Istituto Nazionale per la Ricerca sul Cancro, L.go R. Benzi 10, 16132 Genova, Italy. daniela.pende@istge.it
Castriconi
Roberta
R
Romagnani
Paola
P
Spaggiari
Grazia Maria
GM
Marcenaro
Stefania
S
Dondero
Alessandra
A
Lazzeri
Elena
E
Lasagni
Laura
L
Martini
Stefania
S
Rivera
Paola
P
Capobianco
Andrea
A
Moretta
Lorenzo
L
Moretta
Alessandro
A
Bottino
Cristina
C
D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D023201:CD40 Ligand / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D053646:Interleukin-2 Receptor beta Subunit; D007694:Killer Cells, Natural / Q000276:immunology*; D008024:Ligands; D008070:Lipopolysaccharides / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D055628:Natural Cytotoxicity Triggering Receptor 3; D011070:Poly I-C / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000276:immunology*; D011991:Receptors, Virus / Q000276:immunology*; D015854:Up-Regulation / Q000276:immunology; D000098862:T Lineage-Specific Activation Antigen 1
D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; C508020:IL2RB protein, human; D053646:Interleukin-2 Receptor beta Subunit; D008024:Ligands; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C431710:NCR3 protein, human; D055628:Natural Cytotoxicity Triggering Receptor 3; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2; D011991:Receptors, Virus; C066100:poliovirus receptor; D023201:CD40 Ligand; D011070:Poly I-C