Unusual solute protection against pressure inactivation of yeast alcohol dehydrogenase.
A 3:1 combination of sucrose and glycine provides significantly greater protection against pressure-induced inactivation of yeast alcohol dehydrogenase than either solute alone. Trehalose, alone, gives much greater protection than sucrose alone, but not so in combination with glycine. These are striking new findings that cannot be accounted for by current theories of protein stabilization.
Kidman
Gene
G
Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53705, USA.
Northrop
Dexter B
DB
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D002241:Carbohydrates / Q000502:physiology; D004795:Enzyme Stability / Q000502:physiology; D006874:Hydrostatic Pressure; D011489:Protein Denaturation / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013997:Time Factors
D000596:Amino Acids; D002241:Carbohydrates; D000426:Alcohol Dehydrogenase