title: Use of electrospray ionization mass spectrometry for profiling of crude oil effects on the phospholipid molecular species of two marine bacteria.
abstract: We investigated the membrane lipid composition of two hydrocarbon-degrading gram-negative bacterial strains (Pseudomonas nautica IP 617 and Marinobacter hydrocarbonoclasticus) initially cultured on a soluble substrate, then on petroleum hydrocarbons, and finally taken back onto the soluble substrate. For the two strains, the growth on petroleum and the return to the initial medium showed major, but comparable, qualitative and quantitative modifications of the intact phospholipid molecular species (IPMS) composition. Furthermore, since bacterial membranes are mainly made up of phospholipids, these modifications reflected hydrocarbon assimilation, restoration abilities and membrane fluidity adaptation. The electrospray ionization mass spectrometry (ESI-MS) analysis of intact phospholipid provided some new information (e.g. sn fatty acyl chain distribution) that could not be assessed by the classical fatty acid analysis. Moreover, such information should be particularly helpful with regards to bacterial taxonomy and xenobiotic toxicity studies.
authors.affiliation: Laboratoire de Chimie Analytique de l'Environnement, UMR 6171, IFR PMSE 112, Europôle de l'Arbois, BP 80, 13545 Aix-en-Provence Cedex 4, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mazzella
authors.forename: Josiane
authors.initials: J
authors.lastname: Molinet
authors.forename: Agung Dhamar
authors.initials: AD
authors.lastname: Syakti
authors.forename: Alain
authors.initials: A
authors.lastname: Dodi
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Bertrand
authors.forename: Pierre
authors.initials: P
authors.lastname: Doumenq
mesh_terms: D034241:Alteromonadaceae / Q000187:drug effects* / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry*; D010578:Petroleum / Q000633:toxicity*; D010743:Phospholipids / Q000737:chemistry*; D011548:Pseudomonadaceae / Q000187:drug effects* / Q000378:metabolism; D012623:Seawater / Q000382:microbiology; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010578:Petroleum; D010743:Phospholipids