title: Anti-vimentin antibody detection in recipients of heart-beating and non-heart beating donor kidneys.
abstract: Alternative donor sources include non-heart-beating donors (NHBDs). There donors have been exposed to significant ischemia, so that it is common to utilize machine perfusion to either improve the organs or at least assess their viability. Both prolonged warm ischemia and machine perfusion can potentially damage the vascular endothelium, thereby exposing vimentin to antigenic recognition. The aim of this study was to determine whether anti-vimentin antibodies could be detected in the blood of renal transplant recipients at specific time points after transplant and whether they could be related to the donor source. Fifty-one recipients of NHBD kidneys were compared to 52 recipients of heart-beating donor (HBD) kidneys. All recipients had similar anti-vimentin levels pretransplant. However, at 1 month those kidneys from Maastricht category II NHB donors showed significantly higher levels. At 6 months both Maastricht category II and category III NHB donor recipients displayed significantly higher levels than recipients of HBD kidneys.
authors.affiliation: Renal/Liver Transplant Unit, The Freeman Hospital, High Heaton, Newcastle Upon Tyne NE7 7DN, UK.
authors.forename: H
authors.initials: H
authors.lastname: Mi
authors.forename: V
authors.initials: V
authors.lastname: Carter
authors.forename: A
authors.initials: A
authors.lastname: Gupta
authors.forename: J
authors.initials: J
authors.lastname: Asher
authors.forename: B K
authors.initials: BK
authors.lastname: Shenton
authors.forename: S
authors.initials: S
authors.lastname: Stamp
authors.forename: Y T
authors.initials: YT
authors.lastname: Wong
authors.forename: M A
authors.initials: MA
authors.lastname: Gok
authors.forename: D
authors.initials: D
authors.lastname: Talbot
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D003404:Creatinine / Q000097:blood; D005260:Female; D006323:Heart Arrest; D006339:Heart Rate; D006650:Histocompatibility Testing; D006801:Humans; D007668:Kidney / Q000276:immunology*; D016030:Kidney Transplantation / Q000276:immunology / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D014019:Tissue Donors; D016896:Treatment Outcome; D014746:Vimentin / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D014746:Vimentin; D003404:Creatinine