title: Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption.
abstract: New applications in regenerative biotechnology require the ability to understand and control protein-surface interactions on micrometer and submicrometer length scales. Evidence presented here shows that micropatterned amphiphilic comb polymer films exhibit a pretreatment-dependent behavior with respect to protein adsorption for the proteins fibronectin, laminin, and for serum. A micropatterned surface, consisting of protein-reactive regions, separated by comb polymer, was created and tested for protein adsorption using the surface-sensitive imaging tool TOF-SIMS. Immersion of micropatterned surfaces in solutions of fibronectin or laminin resulted in uniform protein coverage on both the comb polymer and protein-reactive regions. However, preimmersion of similarly patterned surfaces in water for 2 h prior to protein incubation was found to dramatically improve the protein-resistant properties of the comb polymer regions. These results are consistent with poly(ethylene glycol) (PEG) side chain reorientation and/or hydration and poly(methyl methacrylate) (PMMA) backbone segregation away from the interface region.
authors.affiliation: Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA.
authors.forename: Zhanping
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Ma
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Hausner
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Chilkoti
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Beebe
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D001704:Biopolymers / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry; D005898:Glass; D007477:Ions; D046911:Macromolecular Substances / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D005353:Fibronectins; D007477:Ions; D046911:Macromolecular Substances; D011108:Polymers; D011506:Proteins; D011092:Polyethylene Glycols; D019904:Polymethyl Methacrylate