Giardia assemblage A infection and diarrhea in Bangladesh.
Giardia lamblia is the most prevalent human intestinal protozoan worldwide, but only a minority of infections result in diarrhea. We tested here whether the 2 major G. lamblia genotypes, assemblages A and B, differ in their propensity to cause disease. To determine whether an association exists between infection with assemblage A or B and diarrhea, 2534 Bangladeshi patients were enrolled in a case-control study. A total of 322 Giardia infections were identified and assayed for genotype by real-time polymerase chain reaction. Higher odds ratios for diarrhea were observed for assemblage A and A2 infections, whereas higher parasite DNA loads and a higher overall prevalence were observed for assemblage B infections. Our findings indicate that genotypic differences in virulence and fecundity may help to explain why not every Giardia infection results in disease, but they need to be confirmed in other urban populations of the developing world.
Haque
Rashidul
R
Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Roy
Shantanu
S
Kabir
Mamun
M
Stroup
Suzanne E
SE
Mondal
Dinesh
D
Houpt
Eric R
ER
D000818:Animals; D001459:Bangladesh / Q000453:epidemiology; D016022:Case-Control Studies; D016054:DNA, Protozoan / Q000032:analysis; D003967:Diarrhea / Q000453:epidemiology / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology*; D006801:Humans; D016133:Polymerase Chain Reaction; D014774:Virulence
D016054:DNA, Protozoan