title: Phytoremediation of metolachlor by transgenic rice plants expressing human CYP2B6.
abstract: We introduced the human cytochrome P450 gene CYP2B6 into rice plants (Oryza sativa L. cv. Nipponbare), and the CYP2B6-expressing rice plants became more tolerant to various herbicides than nontransgenic Nipponbare rice plants. In particular, CYP2B6 rice plants grown in soil showed tolerance to the chloroacetanilide herbicides alachlor and metolachlor. We evaluated the degradation of metolachlor by CYP2B6 rice plants to confirm the metabolic activity of the introduced CYP2B6. Although both CYP2B6 and nontransgenic Nipponbare rice plants could decrease the amount of metolachlor in plant tissue and culture medium, CYP2B6 rice plants could remove much greater amounts. In a greenhouse, the ability of CYP2B6 rice plants to remove metolachlor was confirmed in large-scale experiments, in which these plants appeared able to decrease residual quantities of metolachlor in water and soil.
authors.affiliation: Plant Biotechnology Department, National Institute of Agrobiological Sciences, 2-1-2, Kannondai, Tsukuba, Ibaraki 305-8602, Japan. shiwak@affrc.go.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawahigashi
authors.forename: Sakiko
authors.initials: S
authors.lastname: Hirose
authors.forename: Hideo
authors.initials: H
authors.lastname: Ohkawa
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Ohkawa
mesh_terms: D000081:Acetamides / Q000032:analysis / Q000378:metabolism*; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics* / Q000378:metabolism*; D065702:Cytochrome P-450 CYP2B6; D004351:Drug Resistance; D006540:Herbicides / Q000032:analysis / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology*; D012987:Soil / Q000032:analysis; D014867:Water / Q000032:analysis
chemical_list: D000081:Acetamides; D006540:Herbicides; D012987:Soil; D014867:Water; D001189:Aryl Hydrocarbon Hydroxylases; C585599:CYP2B6 protein, human; D065702:Cytochrome P-450 CYP2B6; D010089:Oxidoreductases, N-Demethylating; C051786:metolachlor