title: In vivo effect of fluoride-substituted apatite on rat bone.
abstract: Different types of calcium phosphate compounds are commercially available for medical and dental applications as bone substitute materials. Biological apatites contain several kinds of minor elements such as carbonate (CO3), magnesium (Mg), and fluoride (F) in enamel, dentin, and bone. It has been shown that F ion and F-substituted apatite promoted osteoblast proliferation and inhibited osteoclast cell activity. The purpose of this study was to investigate the in vivo rat tibia activity on F-substituted apatite (FAp). Apatites of unsintered calcium deficient apatite (CDA), and FAps, with low, medium, and high F concentrations, were implanted in rat tibia for 1 and 2 weeks. Implanted tissues were embedded in paraffin blocks, stained with hematoxylin-eosin and histomorphometrically observed. Results showed that low F concentration induced better and faster new bone formation in vivo compared to CDA. Therefore the results suggested that F as a minor element in bone rendered a suitable effect on bone formation in vivo.
authors.affiliation: Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8525, Japan. mi8@ccr.tokushima-u.ac.jp
authors.forename: Miho
authors.initials: M
authors.lastname: Inoue
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagatsuka
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Tsujigiwa
authors.forename: Masahisa
authors.initials: M
authors.lastname: Inoue
authors.forename: Racquel Z
authors.initials: RZ
authors.lastname: LeGeros
authors.forename: Toshio
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagai
mesh_terms: D000818:Animals; D001031:Apatites / Q000627:therapeutic use*; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D018786:Bone Substitutes / Q000627:therapeutic use*; D002118:Calcium / Q000627:therapeutic use; D004396:Coloring Agents; D005459:Fluorides / Q000627:therapeutic use; D008297:Male; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D010010:Osteoclasts / Q000187:drug effects / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000187:drug effects* / Q000473:pathology / Q000601:surgery; D013997:Time Factors
chemical_list: D001031:Apatites; D018786:Bone Substitutes; D004396:Coloring Agents; C025105:fluorapatite; D005459:Fluorides; D002118:Calcium