Leishmania (Viannia) panamensis: cloning of the histone H1 genes by representational difference analysis.
We report the use of representational difference analysis to identify genes that have up-regulated expression in the amastigote life-cycle stage of Leishmania (Viannia) panamensis. This simultaneous process of selection and amplification allowed the cloning of several specific DNA fragments. One of them shows a high percentage of similarity with histone H1 genes from other Trypanosomatids and, as expected, is up-regulated in the amastigote life-cycle stage. The gene is present in two copies that are expressed at different levels in promastigotes and also in amastigotes, which seems to be a consequence of their different 3' untranslated regions.
Alzate
Juan F
JF
Programa de Estudio y Control de Enfermedades Tropicales, Universidad de Antioquia, Medellín, Colombia.
Ramírez-Pineda
J Robinson
JR
González
Victor M
VM
Patiño
Edwin
E
Vélez
Ivan D
ID
Jiménez-Ruiz
Antonio
A
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D006657:Histones / Q000235:genetics* / Q000302:isolation & purification; D018334:Leishmania guyanensis / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D016053:RNA, Protozoan / Q000032:analysis; D015854:Up-Regulation
D018076:DNA, Complementary; D006657:Histones; D012333:RNA, Messenger; D016053:RNA, Protozoan