Determinants of product topology in a hybrid Cre-Tn3 resolvase site-specific recombination system.
Many natural DNA site-specific recombination systems achieve directionality and/or selectivity by making recombinants with a specific DNA topology. This property requires that the DNA architecture of the synapse and the mechanism of strand exchange are both under strict control. Previously we reported that Tn3 resolvase-mediated synapsis of the accessory binding sites from the Tn3 recombination site res can impose topological selectivity on Cre/loxP recombination. Here, we show that the topology of these reactions is profoundly affected by subtle changes in the hybrid recombination site les. Reversing the orientation of loxP relative to the res accessory sequence, or adding 4 bp to the DNA between loxP and the accessory sequence, can switch between two-noded and four-noded catenane products. By analysing Holliday junction intermediates, we show that the innate bias in the order of strand exchanges at loxP is maintained despite the changes in topology. We conclude that a specific synaptic structure formed by resolvase and the res accessory sequences permits Cre to align the adjoining loxP sites in several distinct ways, and that resolvase-mediated intertwining of the accessory sequences may be less than has been assumed previously.
Kilbride
Elizabeth A
EA
Institute of Biomedical & Life Sciences, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, UK.
Burke
Mary E
ME
Boocock
Martin R
MR
Stark
W Marshall
WM
D000595:Amino Acid Sequence; D004247:DNA / Q000378:metabolism; D045566:DNA, Cruciform / Q000378:metabolism*; D019426:Integrases / Q000737:chemistry / Q000378:metabolism*; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D045564:Transposon Resolvases / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
D045566:DNA, Cruciform; D014764:Viral Proteins; D004247:DNA; C045073:Cre recombinase; D019426:Integrases; C473491:Tn3 resolvase; D045564:Transposon Resolvases