Gas chromatography/mass spectrometry characterization of urinary metabolites of danazol after oral administration in human.
Danazol (17alpha-pregna-2,4-dien-20-yno [2,3-d]-isoxazol-17beta-ol), is a synthetic derivative of ethisterone, structurally related to stanozolol. For this reason its use as doping agent has been investigated. Danazol (Runch) (200 mg) were orally administered to two healthy male volunteers. Urine samples were collected up to 1-week post-dose. Four new metabolites have been identified in addition to the five previously reported. We propose the monitorization of 6beta-hydroxy-2-hydroxymethyl-1,2-dehydroethisterone and 6beta,16epsilon-dihydroxy-2epsilon-hydroxymethyl-ethisterone by free fraction analysis. In a same way, we proposed to detect the principal isomer of a mono-hydroxylated metabolite of 6beta-hydroxy-2epsilon-hydroxymethylethisterone in the conjugated fraction. We conclude that new metabolites can be included for the detection of danazol abuse since the main metabolite ethisterone is excreted relatively fast in urine.
de Oca Porto
Rodny Montes
RM
Instituto de Medicina del Deporte, Laboratorio Antidoping, Ciudad de la Habana, Cuba. rodnylad@yahoo.com
Fernández
Ariana Rodríguez
AR
Brito
Dayamín Martínez
DM
Vidal
Teresa Correa
TC
Diaz
Ahiram Lopez
AL
D000284:Administration, Oral; D000328:Adult; D045930:Anabolic Agents / Q000008:administration & dosage / Q000652:urine*; D003613:Danazol / Q000008:administration & dosage / Q000652:urine*; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008297:Male; D012015:Reference Standards
D045930:Anabolic Agents; D003613:Danazol