Immunoproteomics of outer membrane proteins and extracellular proteins of Shigella flexneri 2a 2457T.
Shigella flexneri 2a is an important pathogen causing bacillary dysentery in humans. In order to investigate any potential vaccine candidate proteins present in outer membrane proteins (OMPs) and extracellular proteins of S. flexneri 2a 2457T, we use the proteome mapping and database analyzing techniques. A subproteome map and database of OMPs were established first. One hundred and nine of the total 126 marked spots were cut out and processed to MALDI-TOF-MS and PMF. Eighty-seven spots were identified and they represented 55 OMP entries. Furthermore, immunoproteomics analysis of OMPs and extracellular proteins were performed. Total of 34 immunoreactive spots were identified, in which 22 and 12 were from OMPs and extracellular proteins, respectively. Eight novel antigens were found and some of these antigens may be potential vaccine candidate proteins. These results are useful for future studying of pathogenicity, vaccine, and novel antibacterial drugs. Maps and tables of all identified proteins are available on the Internet at www.proteomics.com.cn.
Ying
Tianyi
T
Beijing Institute of Biotechnology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China.
Wang
Hengliang
H
Li
Mingzhu
M
Wang
Junjun
J
Wang
Jie
J
Shi
Zhaoxing
Z
Feng
Erling
E
Liu
Xiankai
X
Su
Guofu
G
Wei
Kaihua
K
Zhang
Xuemin
X
Huang
Peitang
P
Huang
Liuyu
L
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000032:analysis*; D012763:Shigella flexneri / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D020543:Proteome