title: Conjugated linoleic acid-induced fatty liver can be attenuated by combination with docosahexaenoic acid in C57BL/6N mice.
abstract: We investigated the effect of dietary combination of conjugated linoleic acid (CLA) and docosahexaenoic acid (DHA) to attenuate CLA-induced fatty liver in C57BL/6N mice. Mice were fed semisynthetic diets that contained either 6% high linoleic safflower oil (HL-SAF), 4% HL-SAF + 2% CLA, or 3.5% HL-SAF + 2% CLA + 0.5% DHA for 4 weeks. This 4 week feeding of CLA showed hepatic lipid accumulation concomitant with the decrease in adipose tissue weight in mice. However, 0.5% supplementation of DHA to the CLA diet could alleviate fatty liver without decreasing the antiobesity effect of CLA. The CLA diet promoted fatty acid synthesis in the liver, but DHA supplementation significantly attenuated the increase in enzyme activity induced by CLA. On the other hand, serum adipocytokines, leptin and adiponectin, were drastically decreased by CLA feeding, and DHA supplementation did not affect those levels. These results show that DHA supplementation to the CLA diet can attenuate CLA-induced fatty liver through the reduction of hepatic fatty acid synthesis without affecting adipocytokine production in C57BL/6N mice.
authors.affiliation: Department of Applied Biological Sciences, Saga University, Saga 840-8502, Japan. yanagitt@cc.saga-u.ac.jp
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Yanagita
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Wang
authors.forename: Koji
authors.initials: K
authors.lastname: Nagao
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ujino
authors.forename: Nao
authors.initials: N
authors.lastname: Inoue
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage*; D004281:Docosahexaenoic Acids / Q000008:administration & dosage*; D005234:Fatty Liver / Q000139:chemically induced* / Q000517:prevention & control*; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage* / Q000633:toxicity*; D008055:Lipids / Q000032:analysis / Q000097:blood; D008099:Liver / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis; D015430:Weight Gain / Q000187:drug effects
chemical_list: D004041:Dietary Fats; D044243:Linoleic Acids, Conjugated; D008055:Lipids; D012333:RNA, Messenger; D004281:Docosahexaenoic Acids