Sulfurihydrogenibium yellowstonense sp. nov., an extremely thermophilic, facultatively heterotrophic, sulfur-oxidizing bacterium from Yellowstone National Park, and emended descriptions of the genus Sulfurihydrogenibium, Sulfurihydrogenibium subterraneum and Sulfurihydrogenibium azorense.
A novel thermophilic, sulfur-oxidizing Gram-negative bacterium, designated strain SS-5T, was isolated from the Calcite Hot Springs in Yellowstone National Park, USA. The cells were motile rods (1.2-2.8 microm long and 0.6-0.8 microm wide). The new isolate was a facultative heterotroph capable of using elemental sulfur or thiosulfate as an electron donor and O2 (1-18 %; optimum 6 %, v/v) as an electron acceptor. Hydrogen did not support growth. The isolate grew autotrophically with CO2. In addition, strain SS-5T utilized various organic carbon sources such as yeast extract, tryptone, sugars, amino acids and organic acids. Growth was observed between 55 and 78 degrees C (optimum 70 degrees C; 3.5 h doubling time), pH 6.0 and 8.0 (optimum pH 7.5), and 0 and 0.6 % (w/v) NaCl (optimum 0 %). The G+C content of the genomic DNA was 32 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the isolate was a member of the genus Sulfurihydrogenibium. On the basis of the physiological and molecular characteristics of the new isolate, we propose the name Sulfurihydrogenibium yellowstonense sp. nov. with SS-5T (=JCM 12773T=OCM 840T) as the type strain. In addition, emended descriptions of the genus Sulfurihydrogenibium, Sulfurihydrogenibium subterraneum and Sulfurihydrogenibium azorense are proposed.
Nakagawa
S
S
Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
Shtaih
Z
Z
Department of Biology, Portland Sate University, PO Box 751, Portland, OR 97207-0751, USA.
Banta
A
A
Department of Biology, Portland Sate University, PO Box 751, Portland, OR 97207-0751, USA.
Beveridge
T J
TJ
Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Sako
Y
Y
Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
Reysenbach
A-L
AL
Department of Biology, Portland Sate University, PO Box 751, Portland, OR 97207-0751, USA.
D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D006091:Gram-Negative Chemolithotrophic Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure; D045482:Hot Springs / Q000382:microbiology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010802:Phylogeny; D013457:Sulfur Compounds / Q000378:metabolism*; D013696:Temperature; D014871:Water Microbiology; D014959:Wyoming
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D013457:Sulfur Compounds