[Investigations on the application antigens for CFT to the diagnosis of infections in cattle and sheep caused by Chlamydia germs].
Studies were carried out to evaluate and choose the best methods to produce Chlamydial antigens for CFT. The antigens were prepared by the following 4 methods: Beer's method using placentas of ewes infected with Chlamydia: from infected chicken embryos by means of an original own method and by the method of Gyulai at al. (as modified by the author); by the author's method using bull testicles infected with orchitis granulomatosa. Chlamydial antigens obtained in these experiments were active in CFT with some positive Chlamydial sera. The results obtained indicated that the method of Gyulai at al. as modified by the author would be the most useful one under Polish conditions for the production of Chlamydial antigen used in CFT. This test was used for serological diagnosis of Chlamydia infections in cattle and sheep.
Sadowski
J M
JM
Zakład Mikrobiologii, Instytutu Weterynarii w Puławach.
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000517:prevention & control; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000276:immunology / Q000517:prevention & control
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial