title: High-level expression and purification of recombinant E1 enzyme.
abstract: The ubiquitin E1 enzyme is an ATP-dependent enzyme that activates ubiquitin for use in all ubiquitin conjugation pathways. This chapter describes the expression and purification of human E1 enzyme for use in in vitro ubiquitination reactions.
authors.affiliation: Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712-1095, USA.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Beaudenon
authors.forename: Jon M
authors.initials: JM
authors.lastname: Huibregtse
mesh_terms: D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D044763:Ubiquitin-Conjugating Enzymes / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D044763:Ubiquitin-Conjugating Enzymes