title: Proteome analysis of mitochondrial outer membrane from Neurospora crassa.
abstract: The mitochondrial outer membrane mediates numerous interactions between the metabolic and genetic systems of mitochondria and the rest of the eukaryotic cell. We performed a proteomic study to discover novel functions of components of the mitochondrial outer membrane. Proteins of highly pure outer membrane vesicles (OMV) from Neurospora crassa were identified by a combination of LC-MS/MS of tryptic peptide digests and gel electrophoresis of solubilized OMV proteins, followed by their identification using MALDI-MS PMF. Among the 30 proteins found in at least three of four separate analyses were 23 proteins with known functions in the outer membrane. These included components of the import machinery (the TOM and TOB complexes), a pore-forming component (porin), and proteins that control fusion and fission of the organelle. In addition, proteins playing a role in various biosynthetic pathways, whose intracellular location had not been established previously, could be localized to the mitochondrial outer membrane. Thus, the proteome of the outer membrane can help in identifying new mitochondria-related functions.
authors.affiliation: Institute for Physiological Chemistry, Ludwig-Maximillians-Universität, Butenandstrasse 5, 81377 Munich, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Schmitt
authors.forename: Holger
authors.initials: H
authors.lastname: Prokisch
authors.forename: Tilman
authors.initials: T
authors.lastname: Schlunck
authors.forename: David G
authors.initials: DG
authors.lastname: Camp
authors.forename: Uwe
authors.initials: U
authors.lastname: Ahting
authors.forename: Thomas
authors.initials: T
authors.lastname: Waizenegger
authors.forename: Curt
authors.initials: C
authors.lastname: Scharfe
authors.forename: Thomas
authors.initials: T
authors.lastname: Meitinger
authors.forename: Axel
authors.initials: A
authors.lastname: Imhof
authors.forename: Walter
authors.initials: W
authors.lastname: Neupert
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Oefner
authors.forename: Doron
authors.initials: D
authors.lastname: Rapaport
mesh_terms: D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry*; D007425:Intracellular Membranes / Q000737:chemistry*; D008928:Mitochondria / Q000737:chemistry*; D009492:Neurospora crassa / Q000737:chemistry*; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004274:DNA, Recombinant; D005656:Fungal Proteins; D020543:Proteome