title: Catechin derivatives in Jatropha macrantha stems: characterisation and LC/ESI/MS/MS quali-quantitative analysis.
abstract: A phytochemical investigation on methanol extract of stems of Jatropha macrantha led to the isolation of catechin, catechin-7-O-beta-glucopyranoside and proanthocyanidin B-3 along with other catechin polymers. Their structures were established by NMR and ESI/MS experiments. Additionally, an LC/ESI/MS qualitative study and an LC/ESI/MS/MS quantitative study of the phenolic fraction of J. macrantha stems were performed. Combination of high performance liquid chromatography (HPLC) (DAD) with positive electrospray ionisation (ESI) and tandem mass spectrometry (MS/MS) performed with Ion Trap analyser permitted to have qualitative data on catechin derivatives: several other proanthocyanidins were detected. A mixture of proanthocyanidin polymers was characterised by direct introduction ESI-MS analysis. An LC/ESI/MS/MS method was developed and validated for separation and quantification of catechin, catechin-7-O-beta-glucopyranoside and proanthocyanidin B-3. Due to the sensitivity and the repeatability of the assay, we suggest this method as suitable for industrial quality control of raw materials and final products. Quantitative analyses results confirmed that compounds 1-3 are major compounds of the plant and, in particular, proanthocyanidin B-3 appears to be the most abundant.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Facoltá di Farmacia, Universitá di Salerno, Via Ponte don Melillo, 84084 Fisciano, SA, Italy.
authors.forename: Angelyne
authors.initials: A
authors.lastname: Benavides
authors.forename: Paola
authors.initials: P
authors.lastname: Montoro
authors.forename: Carla
authors.initials: C
authors.lastname: Bassarello
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
mesh_terms: D002138:Calibration; D002392:Catechin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D031286:Jatropha / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D018547:Plant Stems / Q000737:chemistry; D044945:Proanthocyanidins / Q000032:analysis; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D044945:Proanthocyanidins; D002392:Catechin