title: Fast and simultaneous detection of prominent natural antioxidants using analytical microsystems for capillary electrophoresis with a glassy carbon electrode: a new gateway to food environments.
abstract: This paper examines for the first time the analytical possibilities of fast and simultaneous detection of prominent natural antioxidants including examples of flavonoids and vitamins using a CE microchip with electrochemical detection (ED). Unpinched injection conditions, zone electrophoretic separation and amperometric detection were carefully assayed and optimised. Analysis involved the zone electrophoretic separation of arbutin, (+)-catechin and ascorbic acid in less than 4 min using a borate buffer (pH 9.0, 50 mM), employing 2 kV as the separation voltage and +1.0 V as the detection potential. In addition, the separation of different 'couples' of natural antioxidants of food significance including (+)-catechin and ascorbic acid, (+)-catechin and rutin, as well as arbutin and phlorizdin is proposed. To demonstrate the potential and future role of CE microsystems, analytical possibilities and a new route in the raw sample analysis are presented. The preliminary results obtained allow the proposal of CE-ED microchips as a real gateway to microanalysis in foods.
authors.affiliation: Dpto. Química Analítica e Ingeniería Química, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
authors.forename: Antonio Javier
authors.initials: AJ
authors.lastname: Blasco
authors.forename: Inés
authors.initials: I
authors.lastname: Barrigas
authors.forename: María Cristina
authors.initials: MC
authors.lastname: González
authors.forename: Alberto
authors.initials: A
authors.lastname: Escarpa
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001104:Arbutin / Q000032:analysis; D002392:Catechin / Q000032:analysis; D004566:Electrodes; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005419:Flavonoids / Q000032:analysis; D005504:Food Analysis / Q000379:methods; D010695:Phlorhizin / Q000032:analysis; D012431:Rutin / Q000032:analysis
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D012431:Rutin; D002392:Catechin; D001104:Arbutin; D010695:Phlorhizin