title: Optical readout of gold nanoparticle-based DNA microarrays without silver enhancement.
abstract: We present a novel readout scheme for gold nanoparticle-based DNA microarrays relying on "Laser-Induced Scattering around a NanoAbsorber". It provides direct counting of individual nanoparticles present on each array spot and stable signals, without any silver enhancement. Given the detection of nanometer-sized particles, which minimize the steric hindrance, the linear dynamic range of the method is particularly large and well suited for microarray detection.
authors.forename: Gerhard A
authors.initials: GA
authors.lastname: Blab
authors.forename: Laurent
authors.initials: L
authors.lastname: Cognet
authors.forename: Stéphane
authors.initials: S
authors.lastname: Berciaud
authors.forename: Isabelle
authors.initials: I
authors.lastname: Alexandre
authors.forename: Dieter
authors.initials: D
authors.lastname: Husar
authors.forename: José
authors.initials: J
authors.lastname: Remacle
authors.forename: Brahim
authors.initials: B
authors.lastname: Lounis
mesh_terms: D000162:Acoustics; D004247:DNA / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D007834:Lasers; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D055095:Optics and Photonics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*
chemical_list: D012834:Silver; D006046:Gold; D004247:DNA