title: Long-oligomer microarray profiling in Neurospora crassa reveals the transcriptional program underlying biochemical and physiological events of conidial germination.
abstract: To test the inferences of spotted microarray technology against a biochemically well-studied process, we performed transcriptional profiling of conidial germination in the filamentous fungus, Neurospora crassa. We first constructed a 70 base oligomer microarray that assays 3366 predicted genes. To estimate the relative gene expression levels and changes in gene expression during conidial germination, we analyzed a circuit design of competitive hybridizations throughout a time course using a Bayesian analysis of gene expression level. Remarkable consistency of mRNA profiles with previously published northern data was observed. Genes were hierarchically clustered into groups with respect to their expression profiles over the time course of conidial germination. A functional classification database was employed to characterize the global picture of gene expression. Consensus motif searches identified a putative regulatory component associated with genes involved in ribosomal biogenesis. Our transcriptional profiling data correlate well with biochemical and physiological processes associated with conidial germination and will facilitate functional predictions of novel genes in N.crassa and other filamentous ascomycete species. Furthermore, our dataset on conidial germination allowed comparisons to transcriptional mechanisms associated with germination processes of diverse propagules, such as teliospores of the phytopathogenic fungus Ustilago maydis and spores of the social amoeba Dictyostelium discoideum.
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA.
authors.forename: Takao
authors.initials: T
authors.lastname: Kasuga
authors.forename: Jeffrey P
authors.initials: JP
authors.lastname: Townsend
authors.forename: Chaoguang
authors.initials: C
authors.lastname: Tian
authors.forename: Luz B
authors.initials: LB
authors.lastname: Gilbert
authors.forename: Gertrud
authors.initials: G
authors.lastname: Mannhaupt
authors.forename: John W
authors.initials: JW
authors.lastname: Taylor
authors.forename: N Louise
authors.initials: NL
authors.lastname: Glass
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D015152:Blotting, Northern; D016000:Cluster Analysis; D030561:Databases, Nucleic Acid; D004023:Dictyostelium / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D005800:Genes, Fungal; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D015203:Reproducibility of Results; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014588:Ustilago / Q000235:genetics
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger