title: Evaluation of rapid diagnostic methods for pediatric viral diarrhea using samples collected in Nepal and Japan.
abstract: We evaluated real time PCR, ELISA and immunochromatography (ICG) test systems for detection of diarrheagenic viruses (Rotavirus, Adenovirus and Norovirus) in pediatric diarrheal fecal samples collected in Kathmandu, Nepal (n = 23) and in Kobe, Japan (n = 25) in summer of 2004. Of the 23 samples collected in Nepal, one (4.3%) was positive for Rotavirus and two (8.7%) for Adenovirus whereas six out of 25 samples (24.0%) collected in Japan were positive for Norovirus. Of the Japanese samples, 11 (44.0%) showed diarrheagenic bacteria (Campylobacter jejuni, Salmolella enteritidis) while six (26.1%) samples from Nepal showed parasites like Ascaris lumbricoides, Giardia intestinalis, Trichuris trchiura and Vampirolepis nana. Viral pathogens were not common during summer season in both Nepal and Japan. Real time RT-PCR was found to be relatively more sensitive compared with ELISA for Norovirus. However, immunological methods are simple and less time consuming (three hours for ELISA, 15 minutes for ICG) and appeared to be useful for rapid diagnosis of viral gastroenteritis especially in developing countries.
authors.affiliation: Department of Microbiology, Kobe Institute of Health, Kobe, Japan. mkurokawa@mvh.biglobe.ne.jp
authors.forename: Manabu
authors.initials: M
authors.lastname: Kurokawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ono
authors.forename: Satoko
authors.initials: S
authors.lastname: Ishiyama
authors.forename: Shiba Kumar
authors.initials: SK
authors.lastname: Rai
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000302:isolation & purification; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D006801:Humans; D007120:Immunochemistry; D007223:Infant; D007231:Infant, Newborn; D007564:Japan / Q000453:epidemiology; D009390:Nepal / Q000453:epidemiology; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification; D012621:Seasons; D013997:Time Factors; D014777:Virus Diseases / Q000175:diagnosis* / Q000453:epidemiology