Expressed protein ligation: a new tool for the biosynthesis of cyclic polypeptides.
The present paper reviews the use of expressed protein ligation for the biosynthesis of backbone cyclized polypeptides. This general method allows the in vivo and in vitro biosynthesis of cyclic polypeptides using recombinant DNA expression techniques. Biosynthetic access to backbone cyclic peptides opens the possibility to generate cell-based combinatorial libraries that can be screened inside living cells for their ability to attenuate or inhibit cellular processes.
Kimura
Richard
R
Chemical Biology and Nuclear Sciences Division, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA.
Camarero
Julio A
JA
D000818:Animals; D004274:DNA, Recombinant / Q000737:chemistry; D006801:Humans; D008956:Models, Chemical; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D010456:Peptides, Cyclic / Q000096:biosynthesis* / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
D004274:DNA, Recombinant; D019151:Peptide Library; D010455:Peptides; D010456:Peptides, Cyclic; D011506:Proteins