title: Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens.
abstract: Intestinal colonization by enteropathogenic and enterohemorrhagic Escherichia coli requires the locus of enterocyte effacement-encoded type III secretion system. We report that NleC and NleD are translocated into host cells via this system. Deletion mutants induced attaching and effacing lesions in vitro, while infection of calves or lambs showed that neither gene was required for colonization.
authors.affiliation: CMMI, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom.
authors.forename: Olivier
authors.initials: O
authors.lastname: Marchés
authors.forename: Siouxsie
authors.initials: S
authors.lastname: Wiles
authors.forename: Francis
authors.initials: F
authors.lastname: Dziva
authors.forename: Roberto M
authors.initials: RM
authors.lastname: La Ragione
authors.forename: Stephanie
authors.initials: S
authors.lastname: Schüller
authors.forename: Angus
authors.initials: A
authors.lastname: Best
authors.forename: Alan D
authors.initials: AD
authors.lastname: Phillips
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Hartland
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Woodward
authors.forename: Mark P
authors.initials: MP
authors.lastname: Stevens
authors.forename: Gad
authors.initials: G
authors.lastname: Frankel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D020895:Enterocytes / Q000378:metabolism* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D008969:Molecular Sequence Data; D009154:Mutation; D021381:Protein Transport; D017422:Sequence Analysis, DNA; D012756:Sheep
chemical_list: D029968:Escherichia coli Proteins; C506688:Z0986 protein, E coli; C506689:Z0990 protein, E coli