title: Determination of free ertapenem in plasma and bronchoalveolar lavage by high-performance liquid chromatography with ultraviolet detection.
abstract: A sensitive assay for the determination of unbound ertapenem in human plasma and bronchoalveolar lavage (BAL) was developed using ultrafiltration of plasma and BAL samples. A rapid HPLC method was used with ultraviolet detection set at a wavelength of 305 nm and a separation on a Prontosil AQ C18 column, with imipenem used as internal standard. This assay was linear over the concentration range of 0.5-100 microg/mL and 0.25-50 microg/mL in plasma and BAL, respectively. Limits of detection and quantitation were respectively 0.05 and 0.25 microg/mL. Validation data for accuracy and precision were CV<2.48 and 8.25%, accuracy in the range 98.1-104.2% and 102.2-108.4%, respectively, for intra and inter-day.
authors.affiliation: Clinical Pharmacokinetic Department, Victor Segalen Bordeaux 2 University and Clinical Pharmacy Haut-Lévêque Hospital, Bordeaux, France.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Gordien
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Boselli
authors.forename: Catherine
authors.initials: C
authors.lastname: Fleureau
authors.forename: Bernard
authors.initials: B
authors.lastname: Allaouchiche
authors.forename: Gérard
authors.initials: G
authors.lastname: Janvier
authors.forename: Olivier
authors.initials: O
authors.lastname: Lalaude
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Saux
authors.forename: Dominique
authors.initials: D
authors.lastname: Breilh
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077727:Ertapenem; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D047090:beta-Lactams / Q000097:blood*
chemical_list: D047090:beta-Lactams; D000077727:Ertapenem