Neurogenesis in rats after focal cerebral ischemia is enhanced by indomethacin.
OBJECTIVE
Newborn cells may participate in repair following ischemic brain injury, but their survival and function may be influenced by inflammation.


METHODS
We investigated the effects of indomethacin, a nonsteroidal antiinflammatory drug, on the fate of newborn cells following transient focal ischemia.


RESULTS
Bromodeoxyuridine (BrdU)-labeled cells, including migrating neuroblasts, were observed in the neighboring striatum and overlying cortex 1 day poststroke. The density of BrdU+ cells labeled with doublecortin, nestin, glial fibrillary acidic protein, or NG2 was increased at 14 and 28 days. Indomethacin increased BrdU+ cells of all lineages and reduced microglial/monocyte activation.


CONCLUSIONS
Indomethacin enhanced the accumulation of newborn cells following stroke.
Hoehn
Benjamin D
BD
Department of Neurosurgery, Stanford University, Stanford, CA, USA.
Palmer
Theo D
TD
Steinberg
Gary K
GK
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D002540:Cerebral Cortex / Q000473:pathology; D003342:Corpus Striatum / Q000473:pathology; D000089662:Doublecortin Protein; D007155:Immunologic Factors / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology*; D007249:Inflammation / Q000276:immunology; D002546:Ischemic Attack, Transient / Q000473:pathology* / Q000503:physiopathology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000473:pathology; D009000:Monocytes; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000894:Anti-Inflammatory Agents, Non-Steroidal; D015415:Biomarkers; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; D007155:Immunologic Factors; D007213:Indomethacin