Induction of cytokines by heat shock proteins and concanavalin A in murine splenocytes.
There has been considerable interest in the effect of heat shock proteins (HSPs) on the innate immune system. Whether HSPs have any direct effects on the activation of lymphocytes is not clear. Using gene expression array, protein array and enzyme-linked immunosorbent assay, we demonstrated that highly purified recombinant murine Hsp60 (rmHsp60), essentially free of lipopolysaccharide contamination, had no effect in the expression of 113 cytokine genes and the release of 22 common cytokines including interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) by murine splenocytes. Likewise, recombinant human Hsp60 (rhHsp60) and rhHsp70 had no effect in the release of IFN-gamma and IL-2. In contrast, concanavalin A induced the expression of a number of cytokine genes and the release of IFN-gamma and IL-2. These results suggest that Hsp60 and Hsp70 do not induce cytokine production by murine splenocytes.
Wang
Yun
Y
Institute for Clinical Research, Washington, DC 20422, USA.
Gao
Baochong
B
Tsan
Min-Fu
MF
D000818:Animals; D019556:COS Cells; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003208:Concanavalin A / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006360:Heat-Shock Proteins / Q000502:physiology*; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology; D040081:Protein Array Analysis; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D016207:Cytokines; D006360:Heat-Shock Proteins; D008070:Lipopolysaccharides; D008934:Mitogens; D003208:Concanavalin A