Optical readout of gold nanoparticle-based DNA microarrays without silver enhancement.
We present a novel readout scheme for gold nanoparticle-based DNA microarrays relying on "Laser-Induced Scattering around a NanoAbsorber". It provides direct counting of individual nanoparticles present on each array spot and stable signals, without any silver enhancement. Given the detection of nanometer-sized particles, which minimize the steric hindrance, the linear dynamic range of the method is particularly large and well suited for microarray detection.
Blab
Gerhard A
GA
Cognet
Laurent
L
Berciaud
Stéphane
S
Alexandre
Isabelle
I
Husar
Dieter
D
Remacle
José
J
Lounis
Brahim
B
D000162:Acoustics; D004247:DNA / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D007834:Lasers; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D055095:Optics and Photonics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*
D012834:Silver; D006046:Gold; D004247:DNA