Locked nucleic acid molecular beacons.
A novel LNA-MB (molecular beacon based on locked nucleic acid bases) has been designed and investigated. It exhibits very high melting temperature and is thermally stable, shows superior single base mismatch discrimination capability, and is stable against digestion by nuclease and has no binding with single-stranded DNA binding proteins. The LNA-MB will be widely useful in a variety of areas, especially for in vivo hybridization studies.
Wang
Lin
L
Center for Research at Bio/nano Interface, Department of Chemistry, UF Genetics Institute, Gainesville, Florida 32611-7200, USA.
Yang
Chaoyong James
CJ
Medley
Colin D
CD
Benner
Steven A
SA
Tan
Weihong
W
D005453:Fluorescence; D020869:Gene Expression Profiling; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry*
D009711:Nucleotides; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; C477371:locked nucleic acid