title: Structural insight into poly(A) binding and catalytic mechanism of human PARN.
abstract: Poly(A)-specific ribonuclease (PARN) is a processive, poly(A)-specific 3' exoribonuclease. The crystal structure of C-terminal truncated human PARN determined in two states (free and RNA-bound forms) reveals that PARNn is folded into two domains, an R3H domain and a nuclease domain similar to those of Pop2p and epsilon186. The high similarity of the active site structures of PARNn and epsilon186 suggests that they may have a similar catalytic mechanism. PARNn forms a tight homodimer, with the R3H domain of one subunit partially enclosing the active site of the other subunit and poly(A) bound in a deep cavity of its nuclease domain in a sequence-nonspecific manner. The R3H domain and, possibly, the cap-binding domain are involved in poly(A) binding but these domains alone do not appear to contribute to poly(A) specificity. Mutations disrupting dimerization abolish both the enzymatic and RNA-binding activities, suggesting that the PARN dimer is a structural and functional unit. The cap-binding domain may act in concert with the R3H domain to amplify the processivity of PARN.
authors.affiliation: Laboratory of Macromolecular Structure, Institute of Molecular and Cell Biology, Proteos, Singapore.
authors.forename: Mousheng
authors.initials: M
authors.lastname: Wu
authors.forename: Michael
authors.initials: M
authors.lastname: Reuter
authors.forename: Hauke
authors.initials: H
authors.lastname: Lilie
authors.forename: Yuying
authors.initials: Y
authors.lastname: Liu
authors.forename: Elmar
authors.initials: E
authors.lastname: Wahle
authors.forename: Haiwei
authors.initials: H
authors.lastname: Song
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000595:Amino Acid Sequence; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D019281:Dimerization; D005095:Exoribonucleases / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011108:Polymers / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D040681:Structural Homology, Protein
chemical_list: D000241:Adenosine; D005095:Exoribonucleases; D011108:Polymers; C069424:polyadenosine