title: Centrobin: a novel daughter centriole-associated protein that is required for centriole duplication.
abstract: In mammalian cells, the centrosome consists of a pair of centrioles and amorphous pericentriolar material. The pair of centrioles, which are the core components of the centrosome, duplicate once per cell cycle. Centrosomes play a pivotal role in orchestrating the formation of the bipolar spindle during mitosis. Recent studies have linked centrosomal activity on centrioles or centriole-associated structures to cytokinesis and cell cycle progression through G1 into the S phase. In this study, we have identified centrobin as a centriole-associated protein that asymmetrically localizes to the daughter centriole. The silencing of centrobin expression by small interfering RNA inhibited centriole duplication and resulted in centrosomes with one or no centriole, demonstrating that centrobin is required for centriole duplication. Furthermore, inhibition of centriole duplication by centrobin depletion led to impaired cytokinesis.
authors.affiliation: Department of Medicine, Division of Cancer Biology, Evanston Northwestern Healthcare Research Institute, Northwestern University Feinberg School of Medicine, Evanston, IL 60201, USA.
authors.forename: Chaozhong
authors.initials: C
authors.lastname: Zou
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Yujie
authors.initials: Y
authors.lastname: Bai
authors.forename: William T
authors.initials: WT
authors.lastname: Gunning
authors.forename: David E
authors.initials: DE
authors.lastname: Wazer
authors.forename: Vimla
authors.initials: V
authors.lastname: Band
authors.forename: Qingshen
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002502:Centrioles / Q000502:physiology* / Q000648:ultrastructure; D018385:Centrosome / Q000648:ultrastructure; D048749:Cytokinesis; D020868:Gene Silencing; D006801:Humans; D051379:Mice; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: C507203:CNTROB protein, human; D018797:Cell Cycle Proteins; D034741:RNA, Small Interfering