title: The majority of human immunodeficiency virus type 1 particles present within splenic germinal centres are produced locally.
abstract: In most stages of human immunodeficiency virus (HIV) infection, cell-free viral particles can be detected in germinal centres (GCs) that are principally retained, in the form of immune complexes, on the surface of follicular dendritic cells (FDCs). The source of this virus remains unknown, although it is agreed that the FDCs themselves are not infected productively. By sequencing HIV viral DNA, genomic RNA and spliced mRNA isolated from individual splenic white pulps, it was shown here that the majority of HIV-1 viral particles are produced locally within the supporting lymphoid structure and do not result from trapping of circulating viruses or immune complexes. These findings underline the exquisite spatial organization of HIV-1 replication in vivo, suggesting a local origin for viruses trapped in splenic GCs.
authors.affiliation: Unité de Rétrovirologie Moléculaire, Institut Pasteur, Paris, France.
authors.forename: Marie-Jeanne
authors.initials: MJ
authors.lastname: Dumaurier
authors.affiliation: Unité de Rétrovirologie Moléculaire, Institut Pasteur, Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Gratton
authors.affiliation: Unité de Rétrovirologie Moléculaire, Institut Pasteur, Paris, France.
authors.forename: Simon
authors.initials: S
authors.lastname: Wain-Hobson
authors.affiliation: Unité de Rétrovirologie Moléculaire, Institut Pasteur, Paris, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Cheynier
mesh_terms: D004279:DNA, Viral; D020566:Dendritic Cells, Follicular / Q000821:virology; D018858:Germinal Center / Q000821:virology*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013154:Spleen / Q000821:virology*
chemical_list: D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral