title: [Expression and significance of the matrix metalloproteinase inhibitor RECK gene in prostate cell strains].
abstract: OBJECTIVE
  To investigate the expression and significance of RECK gene and MMP-9 in prostate cell strains such as BPH-1, DU45, LNCaP and PC-3.
  
  
  METHODS
  The expression of mRNA of RECK and MMP-9 was measured by RT-PCR and RECK protein was evaluated by Western blot.
  
  
  RESULTS
  The mRNA level of the RECK gene in the prostate carcinoma cell strains, such as DU45, LNCaP and PC-3, was lower than that in the benign prostate hyperplasia cell line BPH-1, while MMP-9 had a higher expression. The protein level of RECK in DU-45, LNCaP and PC-3 was lower than that in the BPH-1.
  
  
  CONCLUSIONS
  The RECK gene is supposed to be a kind of tumor suppressor gene, which may act by inhibiting the activity of MMP-9.
authors.affiliation: Department of Urology, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China. dr_zhenyu1976@yahoo.com.cn
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Xu
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Gao
authors.forename: Zheng-pu
authors.initials: ZP
authors.lastname: Zhang
authors.forename: Jing-ping
authors.initials: JP
authors.lastname: Ge
authors.forename: Chuan-liang
authors.initials: CL
authors.lastname: Xu
authors.forename: Ying-hao
authors.initials: YH
authors.lastname: Sun
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D058851:GPI-Linked Proteins; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000235:genetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; C115460:RECK protein, human; D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9