Comparative analyses of genomic imprinting and CpG island-methylation in mouse Murr1 and human MURR1 loci revealed a putative imprinting control region in mice.
Human MURR1 is an orthologue of mouse Murr1 gene, which was previously reported to be imprinted only in adult brain with a maternal allele-predominant expression and to contain another imprinted gene, U2af1-rs1, in the first intron. Human MURR1 was found not to harbor the U2af1-rs1 orthologue and to be expressed biallelically in tissues, including adult brain. Three genes identified around Murr1 and their orthologues around MURR1 were expressed biallelically. These findings suggest that the mouse imprinting locus is limited to a small region and the introduction of U2af1-rs1 in mouse causes the imprinting of this locus. The CpG island (CGI) at U2af1-rs1 with maternal methylation was the only differentially methylated region among CGIs found in these loci. Detailed methylation analyses of the U2af1-rs1 CGI in germ cells led to identification of a region with oocyte-specific methylation. These results suggest that this region is the imprinting control region of the Murr1/U2af1-rs1 locus in mouse.
Zhang
Zhongming
Z
Department of Biomolecular Sciences, Faculty of Medicine, Saga University, Saga 849-8501, Japan.
Joh
Keiichiro
K
Yatsuki
Hitomi
H
Wang
Youdong
Y
Arai
Yuji
Y
Soejima
Hidenobu
H
Higashimoto
Ken
K
Iwasaka
Tsuyoshi
T
Mukai
Tsunehiro
T
D048868:Adaptor Proteins, Signal Transducing; D000483:Alleles; D000818:Animals; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D009865:Oocytes / Q000166:cytology; D009928:Organ Specificity / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*; D012261:Ribonucleoproteins / Q000235:genetics; D000072668:Splicing Factor U2AF
D048868:Adaptor Proteins, Signal Transducing; C480519:COMMD1 protein, human; D002352:Carrier Proteins; C103799:Commd1 protein, mouse; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011506:Proteins; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; C098232:ZRSR2P1 protein, human; C000607495:Zrsr1 protein, mouse