Proteomic analysis of chronic pancreatitis and pancreatic adenocarcinoma.
OBJECTIVE
Markers to differentiate among pancreatic adenocarcinoma, chronic pancreatitis, and normal pancreas would be of significant clinical utility. This study was therefore designed to analyze the proteome of such specimens and identify new candidate proteins for differential diagnosis.


METHODS
A PowerBlot analysis with more than 900 well-characterized antibodies was performed with tissue specimens from patients with chronic pancreatitis, pancreatic adenocarcinoma, and normal pancreas. Differential expression of selected proteins was confirmed on a larger scale by quantitative reverse transcription-polymerase chain reaction and immunohistochemistry using tissue arrays.


RESULTS
A total of 30 and 102 proteins showed significant deregulation between normal pancreas when compared with chronic pancreatitis and pancreatic adenocarcinoma, respectively, and although a substantial proportion were found similarly dysregulated in both chronic pancreatitis and pancreatic adenocarcinoma, several proteins were identified as potential disease-specific markers.


CONCLUSIONS
A large number of proteins are differentially expressed in chronic pancreatitis and pancreatic adenocarcinoma compared with normal pancreas. Among these, expression analysis of UHRF1, ATP7A, and aldehyde oxidase 1 in combination could potentially provide a useful additional diagnostic tool for fine-needle aspirated or cytological specimens obtained during endoscopic investigations.
Crnogorac-Jurcevic
Tatjana
T
Molecular Oncology Unit, Cancer Research UK, Barts and The London School of Medicine and Dentistry, John Vane Science Centre, Charterhouse Square, London, United Kingdom. t.c.jurcevic@qmul.ac.uk
Gangeswaran
Rathi
R
Bhakta
Vipul
V
Capurso
Gabriele
G
Lattimore
Samuel
S
Akada
Masanori
M
Sunamura
Makoto
M
Prime
Wendy
W
Campbell
Fiona
F
Brentnall
Teresa A
TA
Costello
Eithne
E
Neoptolemos
John
J
Lemoine
Nicholas R
NR
D000230:Adenocarcinoma / Q000235:genetics / Q000503:physiopathology*; D014408:Biomarkers, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000503:physiopathology*; D050500:Pancreatitis, Chronic / Q000235:genetics / Q000503:physiopathology*; D040081:Protein Array Analysis; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor