title: Voltage-dependent ion channels in the mouse RPE: comparison with Norrie disease mice.
abstract: We studied electrophysiological properties of cultured retinal pigment epithelial (RPE) cells from mouse and a mouse model for Norrie disease. Wild-type RPE cells revealed the expression of ion channels known from other species: delayed-rectifier K(+) channels composed of Kv1.3 subunits, inward rectifier K(+) channels, Ca(V)1.3 L-type Ca(2+) channels and outwardly rectifying Cl(-) channels. Expression pattern and the ion channel characteristics current density, blocker sensitivity, kinetics and voltage-dependence were compared in cells from wild-type and Norrie mice. Although no significant differences were observed, our study provides a base for future studies on ion channel function and dysfunction in transgenic mouse models.
authors.affiliation: Institut fuer Klinische Physiologie, Charite-Universitaetsmedizin Berlin, Campus Benjamin Franklin, Germany. guido.wollmann@yale.edu
authors.forename: Guido
authors.initials: G
authors.lastname: Wollmann
authors.forename: Steffen
authors.initials: S
authors.lastname: Lenzner
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Berger
authors.forename: Rita
authors.initials: R
authors.lastname: Rosenthal
authors.forename: Mike O
authors.initials: MO
authors.lastname: Karl
authors.forename: Olaf
authors.initials: O
authors.lastname: Strauss
mesh_terms: D000818:Animals; D015153:Blotting, Western; D020746:Calcium Channels, L-Type / Q000378:metabolism; D002478:Cells, Cultured; D018118:Chloride Channels / Q000378:metabolism; D004195:Disease Models, Animal; D015785:Eye Diseases, Hereditary / Q000378:metabolism*; D040181:Genetic Diseases, X-Linked / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D051664:Kv1.3 Potassium Channel / Q000378:metabolism; D008564:Membrane Potentials; D051379:Mice; D018345:Mice, Knockout; D018408:Patch-Clamp Techniques; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism
chemical_list: C485995:Cacna1d protein, mouse; D020746:Calcium Channels, L-Type; D018118:Chloride Channels; D007473:Ion Channels; D051664:Kv1.3 Potassium Channel; D024661:Potassium Channels, Inwardly Rectifying