title: GPR40 gene expression in human pancreas and insulinoma.
abstract: To assess gene expression of a membrane-bound G-protein-coupled fatty acid receptor, GPR40, in the human pancreas and islet cell tumors obtained at surgery were analyzed. The mRNA level of the GPR40 gene in isolated pancreatic islets was approximately 20-fold higher than that in the pancreas, and the level was comparable to or rather higher than that of the sulfonylurea receptor 1 gene, which is known to be expressed abundantly in human pancreatic beta cells. A large amount of GPR40 mRNA was detected in tissue extracts from two cases of insulinoma, whereas the expression was undetectable in glucagonoma or gastrinoma. The present study demonstrates that GPR40 mRNA is expressed predominantly in pancreatic islets in humans and that GPR40 mRNA is expressed solely in human insulinoma among islet cell tumors. These results indicate that GPR40 is probably expressed in pancreatic beta cells in the human pancreas.
authors.affiliation: Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tomita
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Masuzaki
authors.forename: Michio
authors.initials: M
authors.lastname: Noguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iwakura
authors.forename: Junji
authors.initials: J
authors.lastname: Fujikura
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Tanaka
authors.forename: Ken
authors.initials: K
authors.lastname: Ebihara
authors.forename: Junichiro
authors.initials: J
authors.lastname: Kawamura
authors.forename: Izumi
authors.initials: I
authors.lastname: Komoto
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: Koji
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Doi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Shimada
authors.forename: Kiminori
authors.initials: K
authors.lastname: Hosoda
authors.forename: Masayuki
authors.initials: M
authors.lastname: Imamura
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
mesh_terms: D006801:Humans; D007340:Insulinoma / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C473034:FFAR1 protein, human; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled