Peroxisome proliferator-activated receptor alpha L162V polymorphism in nonalcoholic steatohepatitis and genotype 1 hepatitis C virus-related liver steatosis.
BACKGROUND
Peroxisome proliferator-activated receptor alpha (PPARalpha) plays important roles in lipid metabolism. A recently discovered L162V polymorphism of the PPARalpha gene is associated with enhanced transcriptional activity. In this study, the frequency of L162V was investigated in nonalcoholic steatohepatitis (NASH) and genotype 1 hepatitis C virus (HCV)-related liver steatosis.


METHODS
Seventy-two NASH and 141 HCV-infected patients (54 with steatosis, 87 without steatosis) and 119 healthy controls were included. L162V polymorphism of the PPARalpha gene was analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).


RESULTS
PCR and RFLP analysis of the related gene segment was successful in 93%, 96%, and 100% of NASH and HCV-infected patients and controls, respectively. The frequency of the L162V polymorphism was similar in the NASH and HCV-infected patients and controls (5.9%, 3.6%, and 2.5%, respectively). No difference in the frequency of this polymorphism was observed in HCV-infected patients with or without significant liver steatosis. L162V was not associated with obesity, type 2 diabetes mellitus, hypercholesterolemia, or hypertriglyceridemia.


CONCLUSIONS
Neither NASH nor genotype 1 HCV-related liver steatosis seems to be associated with the PPARalpha L162V polymorphism. This polymorphism may have no association with the presence of type 2 diabetes mellitus, obesity, or various blood lipid alterations in NASH and HCV-infected patients.
Verdi
Hasibe
H
Institute of Hepatology, Ankara University, Turkey.
Koytak
Elif Sare
ES
Onder
Oğuz
O
Ergül
Ayça Arslan
AA
Cinar
Kubilay
K
Idilman
Ramazan
R
Erden
Esra
E
Bozdayi
Abdurrahman Mithat
AM
Yurdaydin
Cihan
C
Uzunalimoglu
Ozden
O
Bozkaya
Hakan
H
D000328:Adult; D001483:Base Sequence; D015992:Body Mass Index; D016022:Case-Control Studies; D004247:DNA / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000235:genetics; D005234:Fatty Liver / Q000150:complications / Q000235:genetics* / Q000473:pathology; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics; D019698:Hepatitis C, Chronic / Q000150:complications / Q000235:genetics / Q000821:virology; D006801:Humans; D006949:Hyperlipidemias / Q000150:complications / Q000235:genetics; D008297:Male; D008875:Middle Aged; D047493:PPAR alpha / Q000235:genetics*; D011110:Polymorphism, Genetic
D047493:PPAR alpha; D004247:DNA