title: Dialister micraerophilus sp. nov. and Dialister propionicifaciens sp. nov., isolated from human clinical samples.
abstract: Seventeen anaerobic, Gram-negative, tiny coccobacilli were collected in France from various human clinical samples. Biochemical analyses as well as molecular studies, including 16S rRNA and dnaK gene sequencing, affiliated all the isolates to the genus Dialister. However, 16S rRNA and dnaK gene sequence similarities were below 95.2 and 79.7 %, respectively, when comparisons were performed with the currently described species Dialister pneumosintes and Dialister invisus. Two clusters consisting of 13 and four isolates could be differentiated. 16S rRNA- and dnaK-based phylogeny confirmed that these two clusters represent two novel and distinct lineages within the genus Dialister. Finally, phenotypic, genotypic and phylogenetic data supported the proposal of the two novel species Dialister micraerophilus sp. nov. (type strain ADV 04.01T=AIP 25.04T=CIP 108278T=CCUG 48837T) and Dialister propionicifaciens sp. nov. (type strain ADV 1053.03T=AIP 26.04T=CIP 108336T=CCUG 49291T). The G+C content of the DNA of the D. micraerophilus type strain is 36.3 mol%. On the basis of 16S rRNA gene sequence analysis, 11 isolates originating from Canada could also be affiliated to D. micraerophilus sp. nov., and were included in the species description.
authors.affiliation: Laboratoire de Bactériologie-Virologie, Faculté de Pharmacie, 15 Avenue Charles Flahault, BP 14491, 34060 Montpellier Cedex 5, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Jumas-Bilak
authors.affiliation: Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Jean-Pierre
authors.affiliation: Centre National de Référence des Bactéries Anaérobies et du Botulisme, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Carlier
authors.affiliation: Laboratoire de Bactériologie-Virologie, Faculté de Pharmacie, 15 Avenue Charles Flahault, BP 14491, 34060 Montpellier Cedex 5, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Teyssier
authors.affiliation: National Microbiology Laboratory - Public Health Agency of Canada, 1015 Arlington St, Suite H5040, Winnipeg, MB, Canada R3E 3R2.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Bernard
authors.affiliation: Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Gay
authors.affiliation: Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
authors.forename: Josiane
authors.initials: J
authors.lastname: Campos
authors.affiliation: Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Morio
authors.affiliation: Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Marchandin
mesh_terms: D006801:Humans; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D045854:Veillonellaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D012336:RNA, Ribosomal, 16S