Structural studies of the core region of Aeromonas salmonicida subsp. salmonicida lipopolysaccharide.
The core oligosaccharide structure of the in vivo derived rough phenotype of Aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, CE-MS and one- and two-dimensional NMR analyses and established as the following: [carbohydrate: see text] where R=alpha-D-Galp-(1-->4)-beta-D-GalpNAc-(1--> or alpha-D-Galp-(1--> (approx. ratio 4:3). Comparative CE-MS analysis of A. salmonicida subsp. salmonicida core oligosaccharides from strains A449, 80204-1 and an in vivo rough isolate confirmed that the structure of the core oligosaccharide was conserved among different isolates of A. salmonicida.
Wang
Zhan
Z
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6.
Li
Jianjun
J
Vinogradov
Evgeny
E
Altman
Eleonora
E
D048409:Aeromonas salmonicida / Q000737:chemistry*; D002240:Carbohydrate Sequence; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000737:chemistry*; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular
D008070:Lipopolysaccharides