Allelotyping analysis at chromosome 13q of high-grade prostatic intraepithelial neoplasia and clinically insignificant and significant prostate cancers.
BACKGROUND
Loss of heterozygosity (LOH) at 13q is one of the most common chromosomal alterations in high-stage prostate cancer, yet little is known about genetic changes in earlier-stage prostate cancer.


METHODS
We used five microsatellite markers at 13q14, 21, and 33 to compare LOH frequencies in 51 lesions of high-grade prostatic intraepithelial neoplasia (HGPIN), 21 cases of incidental prostate cancers (IPCs), 31 cases of latent prostate cancers (LPCs), and 102 cases of clinical prostate cancers (CPCs).


RESULTS
The frequency of LOH at 13q with at least 1 marker was 0%, 38%, 56%, and 49% in HGPIN, IPCs, LPCs, and CPCs, respectively. No statistically significant difference was found between the types of prostate cancer. Allelic loss at 13q14 was significantly more frequent in pT4 tumors than in earlier-stage tumors (P=0.011).


CONCLUSIONS
Allelic loss at 13q is not only an important event in the metastasis of prostate cancer, but also associated with the initiation of the tumor.
Lu
Wei
W
Department of Pathology, The Jikei University School of Medicine, Tokyo, Japan. luwei@jikei.ac.jp
Takahashi
Hiroyuki
H
Furusato
Masakuni
M
Maekawa
Suguru
S
Nakano
Masataka
M
Meng
Chenxi
C
Kikuchi
Yasushi
Y
Sudo
Akemi
A
Hano
Hiroshi
H
D000483:Alleles; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002882:Chromosomes, Human, Pair 13; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D009362:Neoplasm Metastasis / Q000235:genetics; D016133:Polymerase Chain Reaction; D019048:Prostatic Intraepithelial Neoplasia / Q000235:genetics* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology