title: Short- or long-term effects of adult myoblast transfer on properties of reinnervated skeletal muscles.
abstract: Skeletal muscle demonstrates a force deficit after repair of injured peripheral nerves. Data from the literature indicate that myoblast transfer enhances recovery of muscle function. Thus, we tested the hypothesis that transfer of adult myoblasts improves the properties of reinnervated rabbit tibialis anterior (TA) muscles in both the short term (4 months) and long term (14 months). Two months after transection and immediate suture of the common peroneal nerve, TA muscles were made to degenerate by cardiotoxin injection and then transplanted with adult myoblasts cultured for 13 days. Under these conditions, muscles studied at 4 months were heavier, contained larger fibers, and developed a significantly higher maximal force than muscles that had only been denervated-reinnervated. In the long term, although muscles made to degenerate were heavier and developed a significantly higher maximal force than denervated-reinnervated muscles, myoblast transfer failed to improve these parameters. However, the overall characteristics of long-term operated muscles tended clearly to approach those of the controls. Taken together, these results may have significant implications in certain orthopedic contexts, particularly after immediate or delayed muscle reinnervation.
authors.affiliation: UMR 866 Différenciation Cellulaire et Croissance, Institut National de la Recherche Agronomique (INRA), Montpellier, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Coulet
authors.forename: Fabien
authors.initials: F
authors.lastname: Lacombe
authors.forename: Cyril
authors.initials: C
authors.lastname: Lazerges
authors.forename: Paul-André
authors.initials: PA
authors.lastname: Daussin
authors.forename: Bernadette
authors.initials: B
authors.lastname: Rossano
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Micallef
authors.forename: Michel
authors.initials: M
authors.lastname: Chammas
authors.forename: Yves
authors.initials: Y
authors.lastname: Reyne
authors.forename: Francis
authors.initials: F
authors.lastname: Bacou
mesh_terms: D000818:Animals; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D007150:Immunohistochemistry; D008297:Male; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology* / Q000294:innervation* / Q000502:physiology; D032448:Myoblasts, Skeletal / Q000737:chemistry / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D018995:Myosin Heavy Chains / Q000032:analysis; D011817:Rabbits; D013997:Time Factors
chemical_list: D018995:Myosin Heavy Chains