title: Histopathological and ultrastructural alterations of induced Yersinia enterocolitica infection in mice.
abstract: Yersinia enterocolitica is an enteric bacterium and infections by this organism are mostly foodborne. It has been implicated to cause enterocolitis, terminal ilitis. diarrhoea, mesenteric lymphadenitis and arthritis in man. Due to paucity of information regarding histopathological and specially ultrastructural alterations in tissues affected, this study was planned with mice as the experimental model. Nine pathogenic Y.enterocoliticaisolates were used to infect 80 albino mice by oral and intraperitoneal route. Pathological alterations were studied by light and electron microscopy. Histopathological examination of intestines showed severe edema, purulent enteritis, goblet cell hyperplasia infiltration of mononuclear cells, thickening of mucosa and necrosis of the tips of villi. Liver showed congestion, hepatocellular degeneration and necrosis, atrophy of hepatocytes and microabcesses. The lungs revealed congestion, edema, haemorrhage and purulent ronchopneumonia, while kidneys showed mild necrotic changes and bacterial emboli in glomeruli. Ultrastructural changes were indicative of mitochondrial degeneration and their loss in kidneys, membranous degeneration with formation of myelin figures in lungs and disorganization, disruption and bleb formation of microvilli in intestines. Y.enterocolitica caused significant histopathological and ultrastructural alterations in experimentally infected mice. Variation in pathogenicity of different strains of Y.enterocolitica was also observed.
authors.affiliation: Department of Microbiology, Christian Medical College, Ludhiana.
authors.forename: Madan
authors.initials: M
authors.lastname: Lal
authors.forename: H
authors.initials: H
authors.lastname: Kaur
authors.forename: L K
authors.initials: LK
authors.lastname: Gupta
authors.forename: N K
authors.initials: NK
authors.lastname: Sood
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D007422:Intestines / Q000473:pathology; D007668:Kidney / Q000473:pathology; D008168:Lung / Q000473:pathology; D051379:Mice; D008854:Microscopy, Electron; D014774:Virulence; D015009:Yersinia Infections / Q000473:pathology*; D015008:Yersinia enterocolitica / Q000472:pathogenicity