Methane fermentation of Japanese cedar wood pretreated with a white rot fungus, Ceriporiopsis subvermispora.
Methane fermentation of Japanese cedar wood was carried out after pretreatment with four strains of white rot fungi, Ceriporiopsis subvermispora ATCC 90467, CZ-3, CBS 347.63 and Pleurocybella porrigens K-2855. These fungi were cultivated on wood chip media with and without wheat bran for 4-8 weeks. The pretreated wood chip was fermented anaerobically with sludge from a sewage treatment plant. Pretreatments with C. subvermispora ATCC 90467, CZ-3 and CBS 347.63 in the presence of wheat bran for 8 weeks decreased 74-76% of beta-O-4 aryl ether linkages in the lignin to accelerate production of methane. After fungal treatments with C. subvermispora ATCC 90467 and subsequent 30-days methane fermentation, the methane yield reached 35 and 25% of the theoretical yield based on the holocellulose contents of the decayed and original wood, respectively. In contrast, treatment with the three strains of C. subvermispora without wheat bran cleaved 15-26% of the linkage and produced 6-9% of methane. There were no significant accelerating effects in wood chips treated with P. porrigens which has a lower ability to decompose the lignin. Thus, it was found that C. subvermispora, with a high ability to decompose aryl ether bonds of lignin, promoted methane fermentation of softwood in the presence of wheat bran.
Amirta
Rudianto
R
Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Kyoto, Japan.
Tanabe
Toshiaki
T
Watanabe
Takahito
T
Honda
Yoichi
Y
Kuwahara
Masaaki
M
Watanabe
Takashi
T
D001487:Basidiomycota / Q000378:metabolism*; D028203:Cedrus / Q000382:microbiology*; D005285:Fermentation / Q000502:physiology; D008031:Lignin / Q000378:metabolism*; D008697:Methane / Q000378:metabolism*; D014934:Wood
D008031:Lignin; D008697:Methane