title: Nitric oxide stimulates the erythrocyte for ascorbate recycling.
abstract: S-Nitrosothiols act as carrier and reservoir of nitric oxide (NO), and release NO under stimulation of ascorbate (Asc). Erythrocyte can regenerate Asc from its oxidised products, thus saving this powerful antioxidant. In this paper the effect of donors of NO, superoxide, and peroxynitrite (SpNONOate, KO(2), and SIN-1, respectively) on the erythrocyte production of Asc was investigated. We report here that NO stimulated, while superoxide and peroxynitrite decreased, the Asc recycling. The NO-stimulating effect on the erythrocyte production of Asc was confirmed by using GSNO, a natural occurring S-nitrosothiol, as NO donor. These data highlight a new property of NO, that is the stimulation of erythrocytes for their Asc recycling. Such a property might contribute to regenerate Asc from its oxidised forms, thus preventing its depletion in the circulation. Temperature and pH significantly affected, both in absence and presence of NO, the recycling of Asc by erythrocytes. We propose that a positive feedback, involving the reciprocal stimulation between Asc and S-nitrosothiols, might enhance productions of Asc by erythrocytes and NO release by circulating S-nitrosothiols.
authors.affiliation: Dipartimento delle Scienze Biologiche, Università di Napoli Federico II, via Mezzocannone 8, 80134 Napoli, Italy.
authors.forename: Maria Stefania
authors.initials: MS
authors.lastname: Spagnuolo
authors.forename: Alessandro
authors.initials: A
authors.lastname: Carlucci
authors.forename: Luisa
authors.initials: L
authors.lastname: Cigliano
authors.forename: Paolo
authors.initials: P
authors.lastname: Abrescia
mesh_terms: D000328:Adult; D001205:Ascorbic Acid / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009589:Nitrogen Oxides / Q000494:pharmacology; D026403:S-Nitrosothiols / Q000494:pharmacology*; D013096:Spermine / Q000031:analogs & derivatives / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism / Q000494:pharmacology; D013696:Temperature
chemical_list: D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; D026403:S-Nitrosothiols; D013481:Superoxides; C039316:potassium superoxide; C091861:spermine nitric oxide complex; D013096:Spermine; D009569:Nitric Oxide; C002385:linsidomine; D008981:Molsidomine; D001205:Ascorbic Acid