title: Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.
abstract: Cell renewal in the adult central nervous system (CNS) is limited, and is blocked in inflammatory brain conditions. We show that both neurogenesis and oligodendrogenesis of adult neural progenitor cells in mice are blocked by inflammation-associated (endotoxin-activated) microglia, but induced by microglia activated by cytokines (IL-4 or low level of IFN-gamma) associated with T-helper cells. Blockage was correlated with up-regulation of microglial production of tumor necrosis factor-alpha. The effect induced by IL-4-activated microglia was mediated, at least in part, by insulin-like growth factor-I. The IL-4-activated microglia showed a bias towards oligodendrogenesis whereas the IFN-gamma-activated microglia showed a bias towards neurogenesis. It thus appears that microglial phenotype critically affects their ability to support or impair cell renewal from adult stem cell.
authors.affiliation: Department of Neurobiology, The Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Oleg
authors.initials: O
authors.lastname: Butovsky
authors.forename: Yaniv
authors.initials: Y
authors.lastname: Ziv
authors.forename: Adi
authors.initials: A
authors.lastname: Schwartz
authors.forename: Gennady
authors.initials: G
authors.lastname: Landa
authors.forename: Adolfo E
authors.initials: AE
authors.lastname: Talpalar
authors.forename: Stefano
authors.initials: S
authors.lastname: Pluchino
authors.forename: Gianvito
authors.initials: G
authors.lastname: Martino
authors.forename: Michal
authors.initials: M
authors.lastname: Schwartz
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D017628:Microglia / Q000166:cytology* / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000235:genetics; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D015847:Interleukin-4; D012313:RNA; D007371:Interferon-gamma