The NIT1 promoter allows inducible and reversible silencing of centrin in Chlamydomonas reinhardtii.
An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA interference in Chlamydomonas reinhardtii.
Koblenz
Bettina
B
Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655, USA.
Lechtreck
Karl-Ferdinand
KF
D000619:Aminohydrolases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000378:metabolism; D020868:Gene Silencing; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000378:metabolism*
D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D000619:Aminohydrolases