Protective effect of capsinoid on lipid peroxidation in rat tissues induced by Fe-NTA.
The activity of a single IP administration (15 or 30 mg/Kg body weight) of vanillyl nonanoate, a simplified analog of capsiate, on ferric nitrilotriacetate (Fe-NTA)-mediated oxidative damage was investigated. A sub-lethal dose of Fe-NTA (15 mg Fe/Kg body weight) was administered IP to rats; animals were sacrificed, and kidney and plasma were collected 1 h after injection. In response to the Fe-NTA administration, a reduction of the levels of total lipids, total unsaturated fatty acids and cholesterol was observed, accompanied by a rise in the concentrations of malondialdehyde (MDA), conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol in plasma and kidney 1 h after administration. A pre-treatment with synthetic capsiate (SCPT) showed remarkable protective effect on the reduction of the levels of total lipids, total unsaturated fatty acids and cholesterol, and the cellular antioxidant vitamin E, inhibiting the increase of MDA, conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol in the plasma and kidney. The protective effect of SCPT and two analogues (vanillyl alcohol and vanillin) during the linoleic acid and cholesterol oxidation was investigated in in vitro systems, providing evidence of definite structure-activity relationships.
Rosa
Antonella
A
Dip. Biologia Sperimentale, Sez. Patologia Sperimentale, Università degli Studi di Cagliari, Cittadella Universitaria, Monserrato, Italy. anrosa@unica.it
Deiana
Monica
M
Corona
Giulia
G
Atzeri
Angela
A
Incani
Alessandra
A
Appendino
Giovanni
G
Dessì
M Assunta
MA
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D001835:Body Weight; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000378:metabolism / Q000494:pharmacology*; D005290:Ferric Compounds / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007653:Ketocholesterols / Q000378:metabolism; D007668:Kidney / Q000187:drug effects; D019787:Linoleic Acid / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects*; D008055:Lipids / Q000737:chemistry; D008315:Malondialdehyde / Q000378:metabolism; D008956:Models, Chemical; D015233:Models, Statistical; D009153:Mutagens / Q000494:pharmacology; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D018384:Oxidative Stress; D010100:Oxygen / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D013997:Time Factors; D014641:Vanillic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
D000975:Antioxidants; D005227:Fatty Acids; D005290:Ferric Compounds; D007653:Ketocholesterols; D008055:Lipids; D009153:Mutagens; C508530:vanillyl nonanoate; D008315:Malondialdehyde; D002784:Cholesterol; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D014641:Vanillic Acid; D009571:Nitrilotriacetic Acid; C003001:7-ketocholesterol; C470213:capsiate; D002211:Capsaicin; D010100:Oxygen; C020326:ferric nitrilotriacetate