Clonotypic analysis of cerebrospinal fluid T cells during disease exacerbation and remission in a patient with multiple sclerosis.
Migration of autoreactive T cells into the central nervous system (CNS) compartment is thought to be an important step in the pathogenesis of multiple sclerosis (MS). To follow the evolution of T cell repertoire in the CNS of a patient with relapsing-remitting MS, we analyzed cerebrospinal fluid (CSF) cells obtained during an acute clinical exacerbation, and subsequent disease remission after 13 months of immunomodulatory therapy. T cell receptor CDR3 region length distribution was significantly altered during the relapse, demonstrating the presence of clonally expanded T cells in the CSF. CDR3 spectratyping is a valuable approach to identify disease-associated T cells in the CNS.
Muraro
Paolo A
PA
Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bldg. 10, Room 5B16, 10 Center Dr MSC1400 Bethesda, MD 20892-1400, USA. murarop@ninds.nih.gov
Cassiani-Ingoni
Riccardo
R
Chung
Katherine
K
Packer
Amy N
AN
Sospedra
Mireia
M
Martin
Roland
R
D000328:Adult; D001324:Autoantigens; D006801:Humans; D007155:Immunologic Factors / Q000627:therapeutic use; D008297:Male; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000235:genetics / Q000276:immunology / Q000473:pathology* / Q000628:therapy; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D013057:Spectrum Analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D001324:Autoantigens; C063762:CDR1 protein, human; D007155:Immunologic Factors; D009419:Nerve Tissue Proteins