title: Effect of eel calcitonin glycosylation on incorporation and channel formation in planar phospholipid membranes.
abstract: Previous studies have shown that different calcitonins interact with planar lipid membranes to form ion channels. In this study, glycosylation of eel calcitonin (eCt) at different positions (Ct3-GlcNAc, Ct14-GlcNAc, Ct20-GlcNAc, Ct26-GlcNAc) is shown to preserve molecular structure and slightly change the energy of incorporation and channel formation in planar lipid bilayers made up of palmitoyl-oleoyl-phosphatidylcholine:dioleoyl phosphatidyl-glycerol (85:15, w:w). The voltage needed to form channels decreased as the attached carbohydrate moved toward the C-terminal (eCt = Ct3-GlcNAc > Ct14-GlcNAc = Ct20-GlcNAc > Ct26-GlcNAc). Interestingly, all the Cts tested maintain the characteristic voltage-conductance dependence found for other Cts, the only channel properties modified concern ion selectivity, that shift toward anion selectivity (eCt = 0.97, Ct3-GlcNAc = 0.49, Ct14-GlcNAc = 0.41, Ct20-GlcNAc = 0.36, Ct26-GlcNAc = 0.47). These aspects would be useful in managing peptide properties for biotechnological and therapeutic applications considering the physiological nature of this peptide.
authors.affiliation: Dipartimento Farmaco-Biologico, Università degli Studi di Bari, I-70126 Bari, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Meleleo
authors.forename: Silvia
authors.initials: S
authors.lastname: Micelli
authors.forename: Kazunori
authors.initials: K
authors.lastname: Toma
authors.forename: Katsuji
authors.initials: K
authors.lastname: Haneda
authors.forename: Enrico
authors.initials: E
authors.lastname: Gallucci
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002116:Calcitonin / Q000737:chemistry* / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002463:Cell Membrane Permeability; D004524:Eels; D006031:Glycosylation; D007473:Ion Channels / Q000096:biosynthesis* / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism*; D017433:Protein Structure, Secondary
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers; D010743:Phospholipids; D002116:Calcitonin