[Study on development of DNA microarrays for human papillomavirus (HPV) diagnosis].
To study the technology for establishing DNA microarrays for the diagnosis of HPV. HPV6, 11, 16 and 18 gene fragments were isolated and printed onto aminosilane-coated glass slides by PixSys 5500 microarray printer as probes to prepare the HPV. HPV samples, after labeled with Cy3 or Cy5, were hybridaized with the microarray followed by scanning for analysis. The experimental condition for preparing the HPV gene chips was investigated and the possibility of HPV genotying using DNA microarrays was discussed. The technique established in this study for preparing HPV DNA microarrays is applicable and has potential clinical application significance.
Liu
Cuihua
C
Institute of Molecular Biology, First Medical University, Guangzhou 510515, China. liuch@fimmu.edu.cn
Ma
Wenli
W
Zhang
Bao
B
Shi
Rong
R
Zheng
Wenling
W
D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D015344:DNA Probes, HPV / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology
D015344:DNA Probes, HPV; D004279:DNA, Viral