title: Crystallization and preliminary X-ray diffraction studies of two myotoxic Lys49-phospholipases A2 complexed with alpha-tocopherol.
abstract: BnSP-7 and BnSP-6, two Lys49-phospholipase A2 isolated from Bothrops neuwiedi pauloensis snake venom, were co-crystallized with alpha-tocopherol and X-ray diffraction data were collected for both complexes (2.2 and 2.6 A). A new "alternative" quaternary conformation for these two complexes compared with all other dimeric Lys49-PLA2 has been observed.
authors.affiliation: Departamento de Física e Biofísica, Instituto de Biociências, UNESP, C. P. 510, CEP 18618-000, Botucatu-SP, Brazil.
authors.forename: Juliana I
authors.initials: JI
authors.lastname: dos Santos
authors.forename: Agnes A S
authors.initials: AA
authors.lastname: Takeda
authors.forename: Daniela P
authors.initials: DP
authors.lastname: Marchi-Salvador
authors.forename: Andreimar M
authors.initials: AM
authors.lastname: Soares
authors.forename: Marcos R M
authors.initials: MR
authors.lastname: Fontes
mesh_terms: D000818:Animals; D001665:Binding Sites; D017837:Bothrops / Q000145:classification; D003435:Crotalid Venoms / Q000737:chemistry; D003460:Crystallization / Q000379:methods*; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D054501:Group II Phospholipases A2; D054513:Group IV Phospholipases A2; D008239:Lysine / Q000737:chemistry / Q000378:metabolism; D010741:Phospholipases A / Q000737:chemistry*; D054467:Phospholipases A2; D030162:Reptilian Proteins; D012910:Snake Venoms; D014961:X-Ray Diffraction / Q000379:methods*; D024502:alpha-Tocopherol / Q000737:chemistry*
chemical_list: D003435:Crotalid Venoms; D030162:Reptilian Proteins; D012910:Snake Venoms; C479860:BnSP-6; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054513:Group IV Phospholipases A2; C410627:Lys49-phospholipase A2, Bothrops neuwiedi pauloensis; D054467:Phospholipases A2; D024502:alpha-Tocopherol; D008239:Lysine; D003545:Cysteine