title: Significant enhancement of fluorescence on hybridization of a molecular beacon to a target DNA in the presence of a site-specific DNA nickase.
abstract: We have developed a simple isothermal (55 degrees C) reaction that permits detection of DNA targets using only two components: a molecular beacon and a site-specific DNA nickase without deoxyribonucleotide triphosphates and primers. The loop sequence of the molecular beacon should contain a DNA nickase recognition site. The nickase-molecular beacon (NMB) combination permits a 100-fold increase in fluorescent signal. The applications of the NMB assay for enhancement of fluorescent signal in some isothermal methods are discussed.
authors.affiliation: Institute of Theoretical and Experimental Biophysics, 142290 Poushchino, Russian Federation.
authors.forename: Ludmila A
authors.initials: LA
authors.lastname: Zheleznaya
authors.forename: Damir S
authors.initials: DS
authors.lastname: Kopein
authors.forename: Evgeniy A
authors.initials: EA
authors.lastname: Rogulin
authors.forename: Sergey I
authors.initials: SI
authors.lastname: Gubanov
authors.forename: Nikolay I
authors.initials: NI
authors.lastname: Matvienko
mesh_terms: D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000737:chemistry*; D005453:Fluorescence; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; D004247:DNA; D003850:Deoxyribonuclease I