title: A panel of 20 highly variable microsatellite polymorphisms in rhesus macaques (Macaca mulatta) selected for pedigree or population genetic analysis.
abstract: This paper reports 20 new microsatellite loci that are highly polymorphic in rhesus macaques (Macaca mulatta). We screened known human microsatellite loci to identify markers that are polymorphic in rhesus macaques, and then selected specific loci that show substantial levels of heterozygosity and robust, reliable amplification. The 20 loci reported here were chosen to include one highly informative microsatellite from each rhesus monkey autosomal chromosome. Fourteen of the 20 polymorphisms are tetranucleotide repeats, and all can be analyzed using standard PCR and electrophoresis procedures. These new rhesus markers have an average of 15.5 alleles per locus and average heterozygosity of 0.83. This panel of DNA polymorphisms will be useful for a variety of different genetic analyses, including pedigree testing, paternity analysis, and population genetic studies. Many of these loci are also likely to be informative in other closely related Old World monkey species.
authors.affiliation: Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA. jrogers@darwin.sfbr.org
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Rogers
authors.forename: Mackenzie
authors.initials: M
authors.lastname: Bergstrom
authors.forename: Roy
authors.initials: R
authors.lastname: Garcia
authors.forename: Jordan
authors.initials: J
authors.lastname: Kaplan
authors.forename: Ann
authors.initials: A
authors.lastname: Arya
authors.forename: Leilah
authors.initials: L
authors.lastname: Novakowski
authors.forename: Zach
authors.initials: Z
authors.lastname: Johnson
authors.forename: Amanda
authors.initials: A
authors.lastname: Vinson
authors.forename: Wendy
authors.initials: W
authors.lastname: Shelledy
mesh_terms: D000483:Alleles; D000818:Animals; D005819:Genetic Markers / Q000235:genetics; D005828:Genetics, Population / Q000379:methods*; D005838:Genotype; D008253:Macaca mulatta / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D010375:Pedigree; D011110:Polymorphism, Genetic / Q000235:genetics*
chemical_list: D005819:Genetic Markers