title: Adsorption and direct probing of fibrinogen and sodium myristate at the air/water interface.
abstract: Fibrinogen (FB), a serum protein, is considered a major inhibitor of lung surfactant function at the lining layer of the alveoli. In this study, the adsorption of aqueous bovine FB at the air/water interface was investigated with tensiometry and directly probed for the first time with ellipsometry and infrared reflection adsorption spectroscopy (IRRAS). The tension results show that FB has moderate surface activity. The surface densities of FB were calculated by using two different ellipsometry models to range from 3+/-0.2 to 17+/-2 mg/m2, for 7.5 to 750 ppm of FB in water at 25 degrees C. Although FB at concentrations from 75 to 750 ppm reached about the same steady surface tension value, the surface densities at 750 ppm FB were substantially larger. The same techniques were used for studying aqueous mixtures of 7.5 to 750 ppm FB with 2 mM of sodium myristate (SM) to investigate a possible interaction of the SM with the protein. The behavior of the FB/SM mixtures was found to be close to that of SM alone. The surface tension of the FB/SM mixtures reached values less than 10 mN/m under surface area oscillation at 20 or 80 rpm. These results and the ellipsometry and the IRRAS results indicate that at a concentration of 2 mM SM, FB, up to 750 ppm, does not inhibit the surfactant surface-tension-lowering function. In certain cases the results demonstrate that FB and SM may act cooperatively in lowering the surface tension.
authors.affiliation: School of Chemical Engineering, Purdue University, West Lafayette, Indiana, 47907-1283, USA.
authors.forename: Erika M
authors.initials: EM
authors.lastname: Hernández
authors.forename: Tze-Lee
authors.initials: TL
authors.lastname: Phang
authors.forename: Xinyun
authors.initials: X
authors.lastname: Wen
authors.forename: Elias I
authors.initials: EI
authors.lastname: Franses
mesh_terms: D000327:Adsorption; D000388:Air; D000818:Animals; D002417:Cattle; D005340:Fibrinogen / Q000737:chemistry*; D019814:Myristic Acid / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared; D013500:Surface Tension; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water; D019814:Myristic Acid; D005340:Fibrinogen