title: Comparative nucleotide sequence analysis of the phosphoprotein gene of peste des petits ruminants vaccine virus of Indian origin.
abstract: The nucleotide sequences of the phosphoprotein (P) gene of peste des petits ruminants (PPRV) vaccine virus (PPRV Sungri/96) belongs to Asian lineage have been determined and the deduced amino acid sequences were compared with another vaccine strain PPRV/Nigeria75/1 and with those of the other morbilliviruses. The 1652 nucleotides of the P gene encode a phosphoprotein of 509 amino acid residues (from nucleotide numbers 60 to 1587), which is 91% identical to that of PPRV/Nigeria75/1. The C protein consists of 177 amino acid residues and is 91% identical with that of PPRV/Nigeria75/1. The conserved mRNA editing site (5'TTAAAAGGGCACAG) was present at positions 742-756 in the P gene, which is conserved in all other morbilliviruses. The CTT trinucleotide sequence is present at the N/P and P/M intergenic region, which is totally conserved in morbilliviruses. This will be the third sequence for the P gene of PPRV since that of the vaccine strain and a wild-type Turkish isolate has been published already.
authors.affiliation: Central Institute of Fisheries Technology, Cochin 682 029, India.
authors.forename: D
authors.initials: D
authors.lastname: Muthuchelvan
authors.forename: A
authors.initials: A
authors.lastname: Sanyal
authors.forename: J
authors.initials: J
authors.lastname: Sarkar
authors.forename: B P
authors.initials: BP
authors.lastname: Sreenivasa
authors.forename: S K
authors.initials: SK
authors.lastname: Bandyopadhyay
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D007194:India; D008969:Molecular Sequence Data; D018107:Peste-des-petits-ruminants virus / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D010750:Phosphoproteins; D014764:Viral Proteins