Proteome analysis of Nelore bull (Bos taurus indicus) seminal plasma.
The Nelore bull (Bos taurus indicus) seminal plasma proteome was analyzed by MALDI-TOF MS and two-dimensional gel electrophoresis. A total of 260 spots were visualized in the 2-DE gel (pI range 3-10) and 13 spots could be identified by peptide mass fingerprinting corresponding to 11 different polypeptides. The results allowed the creation of the first proteomic map of Bos taurus indicus seminal plasma. The roles of the identified proteins in the bull seminal plasma are discussed.
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Brazilian Center for Protein Research, Department of Cell Biology, University of Brasília Brasília/DF, Brazil 70.910-900.
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Sousa
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Ricart
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D000818:Animals; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D008297:Male; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D020543:Proteome; D029589:Seminal Plasma Proteins