Detection and characterization of tet(M) in tetracycline-resistant Listeria strains from human and food-processing origins in Belgium and France.
In the present study, three Listeria monocytogenes strains and one Listeria innocua strain out of a collection of 241 Listeria isolates from human and food-processing sources were found to display resistance to tetracycline (TC) due to the presence of the tet(M) gene. Through sequence analysis, it was shown that tet(M) genes in two of the isolates belong to sequence homology group (SHG) II, a group comprising chromosomally encoded tet(M) genes previously found in Staphylococcus aureus and in lactobacilli. The tet(M) genes of the two other L. monocytogenes strains were associated with a member of the Tn916-Tn1545 family of conjugative transposons and were closely related to SHG III, which harbours enterococcal tet(M) genes associated with Tn916. One of these transposon-containing strains was able to transfer the tet(M) gene to Enterococcus faecalis recipient strain JH2-2. Collectively, these sequence and conjugation data indicate that the acquisition of tet(M) by Listeria strains may be triggered by successive transfers between other Gram-positive organisms.
Bertrand
Sophie
S
Bacteriology Division, Scientific Institute of Public Health, 14 Wytsman street, B-1050 Brussels, Belgium 2,4Laboratory of Microbiology, Faculty of Sciences2 and BCCMTM/LMG Bacteria Collection4, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium 3AFSSA, 31 Av. Tony Garnier, 69364 Lyon cedex 07, France.
Huys
Geert
G
Bacteriology Division, Scientific Institute of Public Health, 14 Wytsman street, B-1050 Brussels, Belgium 2,4Laboratory of Microbiology, Faculty of Sciences2 and BCCMTM/LMG Bacteria Collection4, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium 3AFSSA, 31 Av. Tony Garnier, 69364 Lyon cedex 07, France.
Yde
Marc
M
Bacteriology Division, Scientific Institute of Public Health, 14 Wytsman street, B-1050 Brussels, Belgium 2,4Laboratory of Microbiology, Faculty of Sciences2 and BCCMTM/LMG Bacteria Collection4, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium 3AFSSA, 31 Av. Tony Garnier, 69364 Lyon cedex 07, France.
D'Haene
Klaas
K
Bacteriology Division, Scientific Institute of Public Health, 14 Wytsman street, B-1050 Brussels, Belgium 2,4Laboratory of Microbiology, Faculty of Sciences2 and BCCMTM/LMG Bacteria Collection4, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium 3AFSSA, 31 Av. Tony Garnier, 69364 Lyon cedex 07, France.
Tardy
Florence
F
Bacteriology Division, Scientific Institute of Public Health, 14 Wytsman street, B-1050 Brussels, Belgium 2,4Laboratory of Microbiology, Faculty of Sciences2 and BCCMTM/LMG Bacteria Collection4, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium 3AFSSA, 31 Av. Tony Garnier, 69364 Lyon cedex 07, France.
Vrints
Martine
M
Bacteriology Division, Scientific Institute of Public Health, 14 Wytsman street, B-1050 Brussels, Belgium 2,4Laboratory of Microbiology, Faculty of Sciences2 and BCCMTM/LMG Bacteria Collection4, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium 3AFSSA, 31 Av. Tony Garnier, 69364 Lyon cedex 07, France.
Swings
Jean
J
Bacteriology Division, Scientific Institute of Public Health, 14 Wytsman street, B-1050 Brussels, Belgium 2,4Laboratory of Microbiology, Faculty of Sciences2 and BCCMTM/LMG Bacteria Collection4, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium 3AFSSA, 31 Av. Tony Garnier, 69364 Lyon cedex 07, France.
Collard
Jean-Marc
JM
Bacteriology Division, Scientific Institute of Public Health, 14 Wytsman street, B-1050 Brussels, Belgium 2,4Laboratory of Microbiology, Faculty of Sciences2 and BCCMTM/LMG Bacteria Collection4, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium 3AFSSA, 31 Av. Tony Garnier, 69364 Lyon cedex 07, France.
D001483:Base Sequence; D001530:Belgium; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005516:Food Microbiology; D005602:France; D006801:Humans; D008087:Listeria / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D008088:Listeriosis / Q000453:epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial; D013752:Tetracycline