Inflammatory events in a vascular remodeling model induced by surgical injury to the rat carotid artery.
1.--The aim of our study was to gain insight into the molecular and cellular mechanisms of the inflammatory response to arterial injury in a rat experimental model. 2.--Rats (five for each experimental time) were subjected to brief clamping and longitudinal incision of a carotid artery and monitored for 30 days. Subsequently, Nuclear Factor-kappaB (NF-kappaB) expression was measured by electrophoretic mobility shift assay. Heat shock protein (HSP) 27, HSP47 and HSP70 were evaluated by Western blot. Morphological changes of the vessel wall were investigated by light and electron microscopy. 3.--In injured rat carotid artery NF-kappaB activity started immediately upon injury, and peaked between 2 and 3 weeks later. Western blot showed a significant increase of HSP47 and HSP70 7 days after injury. At 2 weeks postinjury, HSP27 expression peaked. Light microscopy showed a neointima formation, discontinuity of the media layer and a rich infiltrate. Among infiltrating cells electron microscopy identified dendritic-like cells in contact with lymphocytes. 4.--Our model of surgical injury induces a significant inflammatory process characterized by enhanced NF-kappaB activity and HSPs hyperexpression. Dendritic-like cells were for the first time identified as a novel component of tissue repair consequent to acute arterial injury.
Rinaldi
Barbara
B
Department of Experimental Medicine, Excellence Centre on Cardiovascular Diseases, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy. barbara.rinaldi@unina2.it
Romagnoli
Paolo
P
Bacci
Stefano
S
Carnuccio
Rosa
R
Maiuri
Maria Chiara
MC
Donniacuo
Maria
M
Capuano
Annalisa
A
Rossi
Francesco
F
Filippelli
Amelia
A
D000208:Acute Disease; D000818:Animals; D002339:Carotid Arteries / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D020212:Carotid Artery Injuries / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D004730:Endothelium, Vascular / Q000473:pathology / Q000648:ultrastructure; D055551:HSP27 Heat-Shock Proteins; D050958:HSP47 Heat-Shock Proteins / Q000096:biosynthesis; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008854:Microscopy, Electron; D016328:NF-kappa B / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D017539:Tunica Intima / Q000473:pathology; D017540:Tunica Media / Q000473:pathology; D014656:Vascular Surgical Procedures / Q000009:adverse effects*
D055551:HSP27 Heat-Shock Proteins; D050958:HSP47 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486114:Hspb1 protein, rat; D016328:NF-kappa B; D009363:Neoplasm Proteins