title: Novel enzyme immunoassay utilizing lipopolysaccharide-binding protein as a capture molecule for the measurement of chlamydial lipopolysaccharide in serum.
abstract: Chlamydia pneumoniae causes respiratory tract infections. It has a tendency to cause persistent infections, which have been associated with several chronic diseases (e.g., atherosclerosis). At present, there is no reliable method for the diagnosis of chronic C. pneumoniae infection. We developed a novel enzyme immunoassay (EIA) for the quantification of chlamydial lipopolysaccharide (cLPS) in human serum. Serum cLPS was solubilized with detergent and then captured by LPS-binding protein (LBP). LBP-LPS complexes were bound to the solid phase with anti-cLPS monoclonal antibody, and the bound complexes were detected with anti-LBP antibodies. The new method was used to quantify serum cLPS in acute coronary syndrome (ACS) patients (n = 102) and their healthy controls. cLPS was detected in 77.5% of ACS patients and in 52% of controls (P < .001) with geometric mean concentrations of 1.87 and 0.61 microg/mL (P < .001), respectively. The novel cLPS EIA method will provide a potential diagnostic tool for C. pneumoniae infection.
authors.affiliation: Department of Molecular Medicine, National Public Health Institute, Biomedicum, PO Box 104, FIN-00251 Helsinki, Finland. terttu.tiirola@ktl.fi
authors.forename: Terttu
authors.initials: T
authors.lastname: Tiirola
authors.forename: Anne
authors.initials: A
authors.lastname: Jaakkola
authors.forename: Aini
authors.initials: A
authors.lastname: Bloigu
authors.forename: Mika
authors.initials: M
authors.lastname: Paldanius
authors.forename: Juha
authors.initials: J
authors.lastname: Sinisalo
authors.forename: Markku S
authors.initials: MS
authors.lastname: Nieminen
authors.forename: Sylvi
authors.initials: S
authors.lastname: Silvennoinen-Kassinen
authors.forename: Pekka
authors.initials: P
authors.lastname: Saikku
authors.forename: Matti
authors.initials: M
authors.lastname: Jauhiainen
authors.forename: Maija
authors.initials: M
authors.lastname: Leinonen
mesh_terms: D000209:Acute-Phase Proteins / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D002352:Carrier Proteins / Q000276:immunology*; D016993:Chlamydophila pneumoniae / Q000737:chemistry*; D006801:Humans; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000097:blood*; D008562:Membrane Glycoproteins / Q000276:immunology*; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D000907:Antibodies, Bacterial; D002352:Carrier Proteins; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D000099231:Lipopolysaccharide-Binding Protein