title: Effects of Basella alba and Hibiscus macranthus extracts on testosterone production of adult rat and bull Leydig cells.
abstract: OBJECTIVE
  To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the bull, and to develop a novel in vitro test system using Leydig cells from bull testes.
  
  
  METHODS
  The effect of methanol extracts from both plants on testosterone production in isolated Leydig cells from the rat and the bull was analyzed using 125I-radioimmunoassay (125I-RIA). Rat Leydig cells were obtained by common methods, whereas a novel technique was used to purify Leydig cells from bull testes.
  
  
  RESULTS
  Bull testes from the slaughter house were a cheap source of pure Leydig cells. In culture, these cells produced testosterone for 5-6 days, which can be stimulated by human chorionic gonadotrophin (hCG). Basella alba extracts significantly enhanced testosterone production in bull and rat Leydig cells in a concentration-dependent manner. Hibiscus macranthus showed no androgenic effect but was shown to inhibit testosterone production at higher concentrations.
  
  
  CONCLUSIONS
  Leydig cells purified from bull testes can be used as an alternative tool in experimental animal research. Certain fractions of Basella alba extract demonstrated androgenic potential whereas Hibiscus macranthus extracts did not.
authors.affiliation: Department of Biochemistry, University of Yaounde I, P. O. Box 812 Yaounde, Cameroon. pmoundipa@uycdc.uninet.cm
authors.forename: Paul F
authors.initials: PF
authors.lastname: Moundipa
authors.forename: Nathalie Sara E
authors.initials: NS
authors.lastname: Beboy
authors.forename: Fabien
authors.initials: F
authors.lastname: Zelefack
authors.forename: Silvére
authors.initials: S
authors.lastname: Ngouela
authors.forename: Etienne
authors.initials: E
authors.lastname: Tsamo
authors.forename: Wolf-Bernhard
authors.initials: WB
authors.lastname: Schill
authors.forename: Thomas K
authors.initials: TK
authors.lastname: Monsees
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D031584:Hibiscus; D007457:Iodine Radioisotopes; D007985:Leydig Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D000432:Methanol; D010936:Plant Extracts / Q000494:pharmacology*; D010945:Plants, Edible; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents; D013739:Testosterone / Q000096:biosynthesis*
chemical_list: D007457:Iodine Radioisotopes; D010936:Plant Extracts; D012997:Solvents; D013739:Testosterone; D000432:Methanol