A novel processing method for injection-molded polyether-urethane scaffolds. Part 2: cellular interactions.
A large-scale scaffold processing method with injection molding has been successfully developed. Water was used as a foaming agent for the new technique. NaCl was used as a porogen to achieve an open-cell structure. Organic solvents, which are common foaming agents for polyurethane, where not used. Toxic remains in the polymer were therefore prevented. Biocompatibility tested gave a mean optical density of 81% from WST-1 proliferation assay. In comparison to the previously study processing method, hot pressing (Haugen H, Ried V, Brunner M, Will J, Wintermantel E. J Mater Sci: Mater Med2004;15:343-346), the current scaffolds had an increase of 20% of the mean optical density. Cell seeding showed that human fibroblasts adhered to the surface and proliferated. The spread of the adhered fibroblasts was uniform on the surface. A quantitative MTT analysis proved that there was a significant (p < 0.01) increase in the OD level after 7 and 14 days of incubation. This cell layer thickened with increased incubation time from 7 to 14 days (p < 0.05) and had typical fibroblast morphology.
Haugen
H
H
Oral Research Laboratory, Faculty of Dentistry, University of Oslo, NO-0317 Oslo, Norway. h.j.haugen@odont.uio.no
Aigner
J
J
Brunner
M
M
Wintermantel
E
E
D001672:Biocompatible Materials / Q000737:chemistry*; D020811:Biomedical Technology / Q000295:instrumentation / Q000379:methods; D002470:Cell Survival; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D008628:Mercury / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*; D016062:Porosity; D012965:Sodium Chloride / Q000737:chemistry; D013314:Stress, Mechanical; D013718:Tensile Strength; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D011140:Polyurethanes; C115016:polyetherurethane; D014867:Water; D012965:Sodium Chloride; D008628:Mercury