Ultrastructure of rat pup's Purkinje neurons whose mothers were exposed to ethanol during pregnancy and lactation.
This study was intended to investigate the effects of alcohol on the ultrastructure of fetal cerebellar Purkinje cells. Twelve adult female rats of Sprague-Dawley species were utilized. Control and experiment groups were formed. Rats were made pregnant. Rats in experiment group were administered liquid diet containing 6% alcohol. Cerebellums of infant rats were taken on 6th, 8th, and 10th days after birth. For electron microscopy, tissue sections were processed and stained with the usual methods. When control and experiment groups were compared for electron microscopic investigation, degeneration of mithocondria as cristolysis, dilatations of rough endoplasmic reticulum tubuli, and ring-shaped appearance of Golgi apparatus unit were determined. In some groups, nuclear membrane disintegrated. In cytoplasms of Purkinje cells, multivesicular bodies were distinguished. It was determined that liquid diet containing 6% alcohol had toxic effects on Purkinje cells and caused ultrastructural signs of degeneration in these cells.
Köksal
Mete
M
Gazi University Faculty of Medicine Department of Histology and Embryology Beşevler, Ankara, Turkey. mete@dr.com
Ilgaz
Celal
C
Erdogan
Deniz
D
Ozogul
Candan
C
Tong
Ebru K
EK
Kalender
Hakan
H
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002492:Central Nervous System Depressants / Q000009:adverse effects*; D000431:Ethanol / Q000009:adverse effects*; D005260:Female; D007774:Lactation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000379:methods; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011689:Purkinje Cells / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley
D002492:Central Nervous System Depressants; D000431:Ethanol