title: Synergy between xylanases from glycoside hydrolase family 10 and family 11 and a feruloyl esterase in the release of phenolic acids from cereal arabinoxylan.
abstract: The bioconversion of waste residues (by-products) from cereal processing industries requires the cooperation of enzymes able to degrade xylanolytic and cellulosic material. The type A feruloyl esterase from Aspergillus niger, AnFaeA, works synergistically with (1-->4)-beta-D-xylopyranosidases (xylanases) to release monomeric and dimeric ferulic acid (FA) from cereal cell wall-derived material. The esterase was more effective with a family 11 xylanase from Trichoderma viride in releasing FA and with a family 10 xylanase from Thermoascus aurantiacus in releasing the 5,5' form of diferulic acid from arabinoxylan (AX) derived from brewers' spent grain. The converse was found for the release of the phenolic acids from wheat bran-derived AXs. This may be indicative of compositional differences in AXs in cereals.
authors.affiliation: Sustainability of the Food Chain Exploitation Platform, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK. craig.faulds@bbsrc.ac.uk
authors.forename: C B
authors.initials: CB
authors.lastname: Faulds
authors.forename: G
authors.initials: G
authors.lastname: Mandalari
authors.forename: R B
authors.initials: RB
authors.lastname: Lo Curto
authors.forename: G
authors.initials: G
authors.lastname: Bisignano
authors.forename: P
authors.initials: P
authors.lastname: Christakopoulos
authors.forename: K W
authors.initials: KW
authors.lastname: Waldron
mesh_terms: D001203:Ascomycota / Q000201:enzymology; D001234:Aspergillus niger / Q000201:enzymology; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism*; D004357:Drug Synergism; D002523:Edible Grain / Q000737:chemistry / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000378:metabolism; D007220:Industrial Waste; D014242:Trichoderma / Q000201:enzymology; D014990:Xylans / Q000378:metabolism*
chemical_list: D003373:Coumaric Acids; D062385:Hydroxybenzoates; D007220:Industrial Waste; D014990:Xylans; C085118:arabinoxylan; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D043364:Endo-1,4-beta Xylanases; C017616:phenolic acid