title: Capillary affinity electrophoresis using lectins for the analysis of milk oligosaccharide structure and its application to bovine colostrum oligosaccharides.
abstract: Animal colostrum and milk contain complex mixtures of oligosaccharides, which have species-specific profiles. Milk oligosaccharides have various types of structure related to the core structures of glycolipids and N- and O-glycans of glycoproteins and provide a good library to examine the binding of oligosaccharides to various lectins. Recently, we reported a capillary affinity electrophoresis (CAE) method for analyzing the interactions between lectins and complex mixtures of N-linked oligosaccharides prepared from serum glycoproteins. The present paper reports the interactions between 24 milk oligosaccharides and six lectins (PA-I, RCA(120), SBA, WGA, UEA-I, and AAL) analyzed using CAE. Based on the resulting data, we constructed a library that enables us to determine nonreducing terminal monosaccharides, such as Gal, GalNAc, GlcNAc, and Fuc, and to differentiate Gal- or Fuc-linked isomers, such as lacto-N-tetraose, lacto-N-neotetraose, and lacto-N-fucopentaose II and III. In addition, using the library, we show that a combination of the lectins can characterize the neutral oligosaccharides derived from bovine colostrum.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, Kowakae 3-4-1, Higashiosaka-shi, Osaka 577-8502, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nakajima
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Namiko
authors.initials: N
authors.lastname: Matsushita
authors.forename: Tadasu
authors.initials: T
authors.lastname: Urashima
authors.forename: Minoru
authors.initials: M
authors.lastname: Suzuki
authors.forename: Akemi
authors.initials: A
authors.lastname: Suzuki
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kakehi
mesh_terms: D000818:Animals; D002417:Cattle; D003126:Colostrum / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013997:Time Factors
chemical_list: D037102:Lectins; D009844:Oligosaccharides