title: Influence of galactomannans with different molecular weights on the gelation of whey proteins at neutral pH.
abstract: The effect of locust bean gum, a galactomannan, with different molecular weights on the microstructure and viscoelastic properties of heat-induced whey protein gels has been studied using confocal laser scanning microscopy and small-deformation rheology. The results obtained clearly showed that differences in the molecular weight of the polysaccharide have a significant influence on the gel microstructure. Homogeneous mixtures and phase-separated systems, with dispersed droplet and bicontinuous morphologies, were observed by changing the polysaccharide/protein ratio and/or the molecular weight. At 11% whey protein, below the gelation threshold of the protein alone, the presence of the nongelling polysaccharide induces gelation to occur. At higher protein concentration, the main effect of the polysaccharide was a re-enforcement of the gel. However, at the higher molecular weight and concentration of the nongelling polymer, the protein network starts to lose elastic perfection, probably due to the formation of bicontinuous structures with lower connectivity.
authors.affiliation: Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Sónia R
authors.initials: SR
authors.lastname: Monteiro
authors.forename: Cláudia
authors.initials: C
authors.lastname: Tavares
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Evtuguin
authors.forename: Nuno
authors.initials: N
authors.lastname: Moreno
authors.forename: J A
authors.initials: JA
authors.lastname: Lopes da Silva
mesh_terms: D001234:Aspergillus niger / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry; D002845:Chromatography; D004548:Elasticity; D005685:Galactans / Q000737:chemistry; D005690:Galactose / Q000031:analogs & derivatives; D005782:Gels; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046911:Macromolecular Substances / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry; D018613:Microscopy, Confocal; D008894:Milk Proteins / Q000737:chemistry*; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D012212:Rheology; D013696:Temperature; D013997:Time Factors; D014783:Viscosity; D000067816:Whey Proteins
chemical_list: D001672:Biocompatible Materials; D005685:Galactans; D005782:Gels; D046911:Macromolecular Substances; D008351:Mannans; D008894:Milk Proteins; D011108:Polymers; D011134:Polysaccharides; D011506:Proteins; D000067816:Whey Proteins; C012990:galactomannan; D008358:Mannose; D005690:Galactose