title: Catalytic properties of the bifunctional soybean beta-glucan-binding protein, a member of family 81 glycoside hydrolases.
abstract: The beta-glucan-binding protein (GBP) of soybean (Glycine max L.) has been shown to contain two different activities. As part of the plasma membrane-localized pathogen receptor complex, it binds a microbial cell wall elicitor, triggering the activation of defence responses. Additionally, the GBP is able to hydrolyze beta-1,3-glucans, as present in the cell walls of potential pathogens. The substrate specificity, the mode of action, and the stereochemistry of the catalysis have been elucidated. This defines for the first time the inverting mode of the catalytic mechanism of glycoside hydrolases belonging to family 81.
authors.affiliation: Department Biologie I - Botanik, LMU, Menzinger Str. 67, D-80638 München, Germany. fliegmann@lrz.uni-muenchen.de
authors.forename: Judith
authors.initials: J
authors.lastname: Fliegmann
authors.forename: Emilie
authors.initials: E
authors.lastname: Montel
authors.forename: Alma
authors.initials: A
authors.lastname: Djulić
authors.forename: Sylvain
authors.initials: S
authors.lastname: Cottaz
authors.forename: Hugues
authors.initials: H
authors.lastname: Driguez
authors.forename: Jürgen
authors.initials: J
authors.lastname: Ebel
mesh_terms: D002147:Calmodulin / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002384:Catalysis; D003001:Cloning, Molecular; D005936:Glucans / Q000378:metabolism; D006026:Glycoside Hydrolases; D006868:Hydrolysis; D037102:Lectins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010940:Plant Proteins; D013025:Glycine max / Q000737:chemistry*; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; D005936:Glucans; D037102:Lectins; D010940:Plant Proteins; C037937:glucan-binding proteins; D006026:Glycoside Hydrolases