title: Radiation-related cytological changes in oral malignant cells.
abstract: Carcinoma of the oral cavity constitutes approximately 40% of overall malignant tumours in India, with an incidence of about 56,000 cases per year. Radiation responses in oral cancer cells by cytology have not been extensively studied. 102 patients with squamous cell carcinoma of the oral cavity treated by fractionated radiotherapy were studied. Serial scrape smears were taken from each tumour before and after irradiation. The abnormal nuclear counts per 1000 malignant cells were 1.6 and 14.1(p<0.001) for micronucleation, 0.9 and 5.5 (p<0.001) for nuclear budding, 7.6 and 28.1(p<0.001) for binucleation and 2.4 and 11.7(p<0.001) for multinucleation respectively. The study showed a significant rise in radiation induced cytological responses. In addition, radiation changes observed included abnormal and incomplete divisions of a nucleus, fibroblast like appearance cells, and enlargement of nuclear size as well as cytoplasmic granulation. These changes may have an important role to play in understanding the mechanism of cell killing after radiotherapy.
authors.affiliation: The Departments of Pathology and Preventive Medicine Moti Lal Nehru Medical College, Allahabad. mravi1@sancharnet.in
authors.forename: Ravi
authors.initials: R
authors.lastname: Mehrotra
authors.forename: Neeta
authors.initials: N
authors.lastname: Goel
authors.forename: Mamta
authors.initials: M
authors.lastname: Singh
authors.forename: D
authors.initials: D
authors.lastname: Kumar
mesh_terms: D000328:Adult; D000368:Aged; D003581:Cytodiagnosis / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000473:pathology* / Q000532:radiotherapy*