Genetic relationships among wheat genotypes, as revealed by microsatellite markers and pedigree analysis.
Genetic relationships among 20 elite wheat genotypes were studied using microsatellite markers and pedigree analysis. A total of 93 polymorphic bands were obtained with 25 microsatellite primer pairs. Coefficient of parentage (COP) values were calculated using parentage information at the expansion level of 5. The pedigree-based similarity (mean 0.115, range 0.00-0.53) was lower than the similarity assessed using microsatellite markers (mean 0.70, range 0.47-0.91). Similarity estimates were used to construct dendrograms by using the unweighted pair-group method with arithmetic averages (UPGMA). Clustering of genotypes in respect of marker-based similarity revealed two groups. Genotype PBW442 diverged and appeared as distinct from all other genotypes in both marker-based and pedigree-based analysis. The correlation of COP values with genetic similarity values based on microsatellite markers is low (r = 0.285, p < 0.05). The results indicate a need to develop wheat varieties with a diverse genetic background and to incorporate new variability into the existing wheat gene pool.
Sud
Suman
S
Department of Plant Breeding, Genetics and Biotechnology, Punjab Agricultural University, Ludhiana, India. Suman_sood2@yahoo.co.in
Bains
Navtej Singh
NS
Nanda
Govinder Singh
GS
D001947:Breeding / Q000379:methods*; D016000:Cluster Analysis; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics; D010375:Pedigree; D013045:Species Specificity; D014908:Triticum / Q000235:genetics*