Functional analysis of a putative Ca2+ channel gene TaTPC1 from wheat.
The cytosolic free-calcium concentration [Ca2+](cyt) transiently increases under abiotic stresses and the proteins that control this process are gradually disclosed. The Ca2+-permeable channel is one type of these proteins in plants. In the present study, a novel Ca2+-permeable channel gene TaTPC1 encoding a putative membrane protein was cloned from wheat. It was induced under high salinity, polyethylene glycol, low temperature (4 degrees C), and abscisic acid. Expression of TaTPC1 in the yeast mutant lacking CCH1 can recover its growth under lithium stress through functional complementation. TaTPC1 transgenic plants exhibited more stomatal closing in the presence of Ca2+ than the control, supporting a role for the calcium channel in regulating plant responses to environmental change.
Wang
Yu-Jun
YJ
National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Yu
Jia-Ning
JN
Chen
Tao
T
Zhang
Zhi-Gang
ZG
Hao
Yu-Jun
YJ
Zhang
Jin-Song
JS
Chen
Shou-Yi
SY
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D003001:Cloning, Molecular; D015870:Gene Expression; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D030821:Plants, Genetically Modified / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
D015220:Calcium Channels; D010940:Plant Proteins; D002118:Calcium