title: Nitric oxide protects against mitochondrial permeabilization induced by glutathione depletion: role of S-nitrosylation?
abstract: Nitric oxide (NO) is known to mediate a multitude of biological effects including inhibition of respiration at cytochrome c oxidase (COX), formation of peroxynitrite (ONOO-) by reaction with mitochondrial superoxide (O2*-), and S-nitrosylation of proteins. In this study, we investigated pathways of NO metabolism in lymphoblastic leukemic CEM cells in response to glutathione (GSH) depletion. We found that NO blocked mitochondrial protein thiol oxidation, membrane permeabilization, and cell death. The effects of NO were: (1) independent of respiratory chain inhibition since protection was also observed in CEM cells lacking mitochondrial DNA (rho0) which do not possess a functional respiratory chain and (2) independent of ONOO- formation since nitrotyrosine (a marker for ONOO- formation) was not detected in extracts from cells treated with NO after GSH depletion. However, NO increased the level of mitochondrial protein S-nitrosylation (SNO) determined by the Biotin Switch assay and by the release of NO from mitochondrial fractions treated with mercuric chloride (which cleaves SNO bonds to release NO). In conclusion, these results indicate that NO blocks cell death after GSH depletion by preserving the redox status of mitochondrial protein thiols probably by a mechanism that involves S-nitrosylation of mitochondrial protein thiols.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597, Singapore.
authors.forename: Matthew
authors.initials: M
authors.lastname: Whiteman
authors.forename: Yee Liu
authors.initials: YL
authors.lastname: Chua
authors.forename: Dawei
authors.initials: D
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Duan
authors.forename: Yih-Cherng
authors.initials: YC
authors.lastname: Liou
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Armstrong
mesh_terms: D016923:Cell Death; D045744:Cell Line, Tumor; D005978:Glutathione / Q000172:deficiency*; D006801:Humans; D008627:Mercuric Chloride / Q000494:pharmacology; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D024101:Mitochondrial Proteins / Q000378:metabolism; D009569:Nitric Oxide / Q000502:physiology*; D010084:Oxidation-Reduction; D010539:Permeability; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; D013438:Sulfhydryl Compounds; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D008627:Mercuric Chloride; D005978:Glutathione