In vivo and in vitro comparison of the effects of FGF-2 null and haplo-insufficiency on bone formation in mice.
We previously reported that deletion of the Fgf2 gene (Fgf2-/-) resulted in decreased bone mass in adult mice. This study examines the effect of haplo-insuffiency (Fgf2+/-) on bone loss in vertebrae from these mutant mice. Fgf2+/+ mice attained peak bone mass at 8-9 months of age. In contrast BMD was significantly reduced in vertebrae from adult (8-9) Fgf2+/- mice. Exogenous FGF-2 rescued reduced bone nodule formation in Fgf2+/- and Fgf2-/- cultures. Runx2 mRNA was reduced in cultures from Fgf2+/- and Fgf2-/- mice. FGF receptor2 mRNA and protein were markedly reduced in Fgf2+/- and Fgf2-/- mice. Decreased bone formation in Fgf2 mutant mice may correlate with impaired FGFR signaling, decreased Runx2 gene expression.
Naganawa
T
T
Department of Medicine, University of Connecticut Health Center, Farmington, CT 06030-0524, USA.
Xiao
L
L
Abogunde
E
E
Sobue
T
T
Kalajzic
I
I
Sabbieti
M
M
Agas
D
D
Hurley
M M
MM
D000818:Animals; D015415:Biomarkers; D001835:Body Weight; D015519:Bone Density; D002454:Cell Differentiation; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D016222:Fibroblast Growth Factor 2 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D006239:Haplotypes; D051379:Mice; D018345:Mice, Knockout; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D046509:Tissue Culture Techniques
D015415:Biomarkers; D050659:Core Binding Factor Alpha 1 Subunit; D012333:RNA, Messenger; C493716:Runx2 protein, mouse; D016222:Fibroblast Growth Factor 2