Tat(28-35)SL8-specific CD8+ T lymphocytes are more effective than Gag(181-189)CM9-specific CD8+ T lymphocytes at suppressing simian immunodeficiency virus replication in a functional in vitro assay.
Epitope-specific CD8+ T lymphocytes may play an important role in controlling human immunodeficiency virus (HIV)/simian immunodeficiency virus replication. Unfortunately, standard cellular assays do not measure the antiviral efficacy (the ability to suppress virus replication) of CD8+ T lymphocytes. Certain epitope-specific CD8+ T lymphocytes may be better than others at suppressing viral replication. We compared the antiviral efficacy of two immunodominant CD8+ T lymphocyte responses--Tat(28-35)SL8 and Gag(181-189)CM9--by using a functional in vitro assay. Viral suppression by Tat-specific CD8+ T lymphocytes was consistently greater than that of Gag-specific CD8+ T lymphocytes. Such differences in antigen-specific CD8+-T-lymphocyte efficacy may be important for selecting CD8+ T lymphocyte epitopes for inclusion in future HIV vaccines.
Loffredo
John T
JT
Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715, USA
Rakasz
Eva G
EG
Giraldo
Juan Pablo
JP
Spencer
Sean P
SP
Grafton
Kelly K
KK
Martin
Sarah R
SR
Napoé
Gnankang
G
Yant
Levi J
LJ
Wilson
Nancy A
NA
Watkins
David I
DI
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D015683:Gene Products, gag / Q000276:immunology; D015696:Gene Products, tat / Q000276:immunology; D008253:Macaca mulatta; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D014779:Virus Replication / Q000276:immunology* / Q000502:physiology
D000956:Antigens, Viral; D018984:Epitopes, T-Lymphocyte; D015683:Gene Products, gag; D015696:Gene Products, tat