Oxidized tissue proteins after intestinal reperfusion injury in rats.
OBJECTIVE
To analyse if the carbonyl proteins measurement could be validated as a method that allows the identification of an intestinal oxidative stress after ischemia and reperfusion injury.


METHODS
Twenty-five male Wistar rats (n = 21) weighting 200 to 250 g were divided into three groups. Group I--control (n = 10). Group II--sham (n = 5) and Group III (n = 10) subjected to 60 minutes of intestinal ischemia and equal period of reperfusion. For this purpose it was clamped the superior mesenteric artery in its distal third. Histological changes and carbonyl protein levels were determined in the samples of all groups. In group III, samples of both normal and reperfused ileal segment were studied.


RESULTS
All the reperfused segments showed mucosal and submucosal swelling and inflammatory infiltrate of the lamina propria. Levels of carbonyl protein rose in group III, including in the non-ischemic segments. The sensitivity and specificity of the carbonyl protein tissue levels were respectively 94% and 88%.


CONCLUSIONS
The carbonyl protein method is a useful biologic marker of oxidative stress after the phenomenon of intestinal ischemia and reperfusion in rats. It was also noteworthy that the effects of oxidative stress could be seen far from the locus of the primary injury.
Schanaider
Alberto
A
Department of Surgery, Faculty of Medicine, Federal University of Rio de Janeiro (UFRJ), Brazil. alberto-sch@ig.com.br
Menezes
Vinícius José Martinho Toledo
VJ
Borchardt
Aline Cury
AC
Oliveira
Pedro Lagerblad de
PL
Madi
Kalil
K
D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002255:Carbonic Acid / Q000032:analysis* / Q000378:metabolism; D007082:Ileum / Q000098:blood supply; D007422:Intestines / Q000098:blood supply*; D008297:Male; D018384:Oxidative Stress; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D012680:Sensitivity and Specificity
D015415:Biomarkers; D011506:Proteins; D002255:Carbonic Acid