Probing protein tertiary structure with amidination.
A chemical derivatization method, amidination, that has recently been effectively employed in peptide mass spectrometry experiments is used to covalently modify lysines in several standard proteins. Protein and peptide mass spectra identify sites at which the reaction does or does not occur. This is therefore a rapid approach to elucidate solvent-accessible regions of folded proteins.
Janecki
Dariusz J
DJ
Department of Chemistry, Indiana University, Bloomington, 47405, USA.
Beardsley
Richard L
RL
Reilly
James P
JP
D000586:Amination; D000588:Amines / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D024402:Carbonic Anhydrase II / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D012997:Solvents; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism
D000588:Amines; D006454:Hemoglobins; D011506:Proteins; D012997:Solvents; D025801:Ubiquitin; D024402:Carbonic Anhydrase II; D003545:Cysteine