Controlled recovery of the transcription of nanoparticle-bound DNA by intracellular concentrations of glutathione.
Positively charged trimethylammonium-functionalized mixed monolayer protected clusters (MMPCs) bind DNA through complementary electrostatic interactions, resulting in complete inhibition of DNA transcription of T7 RNA polymerase. DNA was released from the nanoparticle by intracellular concentrations of glutathione, resulting in efficient transcription. The restoration of RNA production was dose-dependent in terms of GSH, with considerable control of the release process possible through variation in monolayer structure. This work presents a new approach to controlled release of DNA, with potential applications in the creation of transfection vectors and gene regulation systems.
Han
Gang
G
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, USA.
Chari
Nandini S
NS
Verma
Ayush
A
Hong
Rui
R
Martin
Craig T
CT
Rotello
Vincent M
VM
D004247:DNA / Q000008:administration & dosage* / Q000235:genetics / Q000493:pharmacokinetics; D012321:DNA-Directed RNA Polymerases / Q000008:administration & dosage / Q000235:genetics / Q000493:pharmacokinetics; D003692:Delayed-Action Preparations; D005978:Glutathione / Q000494:pharmacology*; D049329:Nanostructures; D012313:RNA / Q000096:biosynthesis; D055672:Static Electricity; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection / Q000379:methods*; D014764:Viral Proteins / Q000008:administration & dosage / Q000235:genetics / Q000493:pharmacokinetics
D003692:Delayed-Action Preparations; D014764:Viral Proteins; D012313:RNA; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D005978:Glutathione