PCR detection of virulence-associated genes in Campylobacter jejuni strains with differential ability to invade Caco-2 cells and to colonize the chick gut.
In this study, the presence of 20 putative virulence genes was examined in 11 Campylobacter jejuni isolates with different colonization and invasion abilities as determined in a chick colonization model and on Caco-2 cells, respectively. The majority of the genes were detected in all strains. Among them, there were genes of the flagellar secretion apparatus like flhA, flhB, flgB, flgE2, the flagellin genes flaA and flaB, invasion-associated genes like ciaB and iamA, the cytotoxin genes cdtA-C, the adhesion related gene cadF, and some genes involved in the colonization process (docA, docB). The plasmid gene virB11 could not be detected in any strain. Specific differences between the isolates were observed only in genes cgtB and wlaN involved in lipo-oligosaccharide (LOS) biosynthesis. The gene cgtB was only detectable in three of five strains with strong colonization and invasion abilities. Probably, wlaN can overcome the lack of cgtB in the two cgtB- isolates.
Müller
J
J
Friedrich Loeffler Institute, Institute of Molecular Pathogenesis, Jena, Naumburger Str. 96a, 07743 Jena, Germany. jens.mueller@fli.bund.de
Schulze
F
F
Müller
W
W
Hänel
I
I
D000818:Animals; D018938:Caco-2 Cells; D016123:Campylobacter jejuni / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D002645:Chickens; D017931:DNA Primers / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000145:classification / Q000235:genetics*
D017931:DNA Primers; D037521:Virulence Factors