title: Paenibacillus alkaliterrae sp. nov., isolated from an alkaline soil in Korea.
abstract: A Gram-positive, rod-shaped, motile and endospore-forming bacterial strain, KSL-134T, was isolated from an alkaline soil in Korea, and its taxonomic position was investigated by a polyphasic study. Strain KSL-134T grew optimally at pH 7 x 5 and 30 degrees C. Its cell wall peptidoglycan contained meso-diaminopimelic acid. Strain KSL-134T was characterized as having MK-7 as the predominant menaquinone and anteiso-C15 : 0 as the major fatty acid. The DNA G+C content was 49 x 4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KSL-134T formed a distinct lineage within the evolutionary radiation encompassed by the genus Paenibacillus. Similarity levels between the 16S rRNA gene sequence of strain KSL-134T and those of the type strains of recognized Paenibacillus species ranged from 90 x 4 to 96 x 5 %. DNA-DNA relatedness levels and some differential phenotypic properties were enough to distinguish strain KSL-134T from several phylogenetically related Paenibacillus species. On the basis of phenotypic and phylogenetic data, strain KSL-134T (=KCTC 3956T=DSM 17040T) was classified in the genus Paenibacillus as a member of a novel species, for which the name Paenibacillus alkaliterrae sp. nov. is proposed.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
authors.forename: So-Jung
authors.initials: SJ
authors.lastname: Kang
authors.affiliation: The Center for Traditional Microorganism Resources, Keimyung University, Shindang-Dong, Dalseo-gu, Daegu, Korea.
authors.forename: Soo-Hwan
authors.initials: SH
authors.lastname: Yeo
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
authors.forename: Tae-Kwang
authors.initials: TK
authors.lastname: Oh
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S