G-tail telomere HPA: simple measurement of human single-stranded telomeric overhangs.
Accurate measurement of telomeric 3'-overhang (G-tail) lengths is essential for investigation of the biological effects of telomere dysfunction. G-tail telomere hybridization protection assay (Gt-telomere HPA) has the advantages of being simple to perform, accurate and highly sensitive for G tails as short as 20 nucleotides. Furthermore, Gt-telomere HPA is specific and quantitative for human G tails, and can be used to assay cell lysates as well as genomic DNA.
Tahara
Hidetoshi
H
Department of Cellular and Molecular Biology, Division of Integrated Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima City, Hiroshima 734-8551, Japan. toshi@hiroshima-u.ac.jp
Kusunoki
Mayuko
M
Yamanaka
Yusuke
Y
Matsumura
Shusaku
S
Ide
Toshinori
T
D001483:Base Sequence; D045744:Cell Line, Tumor; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012680:Sensitivity and Specificity; D016615:Telomere / Q000737:chemistry / Q000235:genetics*
D004277:DNA, Single-Stranded