title: The heme-binding lipoprotein (HbpA) of Haemophilus influenzae: role in heme utilization.
abstract: Haemophilus influenzae has an absolute growth requirement for heme and a heme binding lipoprotein (HbpA) has been implicated in the utilization of this essential nutrient. HbpA was identified by examining clones from an H. influenzae genomic library that caused Escherichia coli harboring the clone to bind heme. However, HbpA has not been shown to mediate heme acquisition in H. influenzae. We constructed an insertional mutation of hbpA in a nontypeable H. influenzae strain and demonstrated a role for the gene in utilization of multiple heme sources. This is the first report confirming a role for HbpA in utilization of heme.
authors.affiliation: Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Morton
authors.forename: Larissa L
authors.initials: LL
authors.lastname: Madore
authors.forename: Ann
authors.initials: A
authors.lastname: Smith
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Vanwagoner
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Seale
authors.forename: Paul W
authors.initials: PW
authors.lastname: Whitby
authors.forename: Terrence L
authors.initials: TL
authors.lastname: Stull
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D015321:Gene Rearrangement; D006193:Haemophilus influenzae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D006418:Heme / Q000378:metabolism*; D008074:Lipoproteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C097952:HbpA protein, Haemophilus influenzae; D008074:Lipoproteins; D006418:Heme