The effect of sonication on simulated osteoarthritis. Part I: effects of 1 MHz ultrasound on uptake of hyaluronan into the rabbit synovium.
High molecular weight (MW) hyaluronan (HA) preparation is considered to be more biologically active than HAs of lower MWs. However, many of the HA preparations currently used to treat osteoarthritis (OA) have lower MWs by the enhanced penetration of HA molecules into the synovial lining cells. In this study, we determined the effectiveness of sonophoresis on the delivery of high MW HA into synovial membrane using an animal model of OA. A total of 1000 kDa (HA1000) and 3000 kDa (HA3000) HA were labeled with fluorescein and injected into the knees of rabbits. Low-intensity continuous ultrasound at 1 MHz, 400 mW/cm2 was applied to the knees for 10 min treatment bid. Synovial fluid analysis revealed increased absorption and fluorescence microscopy showed deeper penetration of both HA1000 and HA3000, more so with the latter. Histologic examination indicated that ultrasound treatment resulted in no apparent damage to the synovial membrane. These results suggest that simultaneous sonication with HA injection might compensate for the short half-life of HA. Consequently, this dual treatment would render HA a far more effective tool in the management of OA.
Park
So Ra
SR
Department of Physiology, College of Medicine, Inha University, Incheon, Korea.
Jang
Kyung Wha
KW
Park
Sang-Hyug
SH
Cho
Hong Sik
HS
Jin
Cheng Zhe
CZ
Choi
Min Joo
MJ
Chung
Soo Il
SI
Min
Byoung-Hyun
BH
D000818:Animals; D005260:Female; D006207:Half-Life; D006614:Hindlimb; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D023421:Models, Animal; D008970:Molecular Weight; D020370:Osteoarthritis, Knee / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D011817:Rabbits; D013010:Sonication; D013582:Synovial Fluid / Q000737:chemistry / Q000378:metabolism; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology
D006820:Hyaluronic Acid