Intratracheal and intranasal immunization with ovalbumin conjugated with Bacillus firmus as a carrier in mice.
Inactivated Bacillus firmus (BF), G+ nonpathogenic bacterium of the external environment, was coupled to ovalbumin (OVA) and used in immunization experiments as antigen carrier. Balb/c mice were immunized thrice intra-tracheally and intra-nasally with conjugates of OVA and BF. Surprisingly, administration of OVA-BF conjugates inhibited anti-OVA IgG response in both sera and mucosal secretions if compared to an exposure to OVA alone. The suppression of antigen-specific antibody production was accompanied by promotion of TH1 phenotype.
Mlcková
P
P
Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, Prague, Czechia.
Polácek
M
M
Cechová
D
D
Marusková
L
L
Stanková
I
I
Chalupná
P
P
Novotná
O
O
Julák
J
J
Prokesová
L
L
D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D000941:Antigens / Q000008:administration & dosage*; D001407:Bacillus / Q000276:immunology*; D004337:Drug Carriers; D005260:Female; D018928:Immunity, Mucosal; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D014132:Trachea
D000907:Antibodies, Bacterial; D000941:Antigens; D004337:Drug Carriers; D007074:Immunoglobulin G; D010047:Ovalbumin