[The role of caveolae in shear stress-induced endothelial nitric-oxide synthase activation].
This article deals with the influence of shear stress on endothelial NO synthesis, and the role of caveolae in shear stress-induced eNOS activation. Human umbilical vascular endothelial cells (HUVEC) were cultured and exposed to different levels of laminal shear stress and Filipin, the perfused cultures were collected, and NO(2-)/NO(3-) was detected using nitrate reduction method. The structure of caveolae was observed through transmission electron microscopy (TEM). The level of NO(2-)-/NO(3-) was found to increase with the elevation of shear stress level (P < 0.01). It was the highest at 1.5 N/m2. After treatment with Filipin, the level of NO produced by HUVEC decreased significantly (P < 0.01), but after recovery and shear without Filipin, the level of NO synthesis bounded back (P < 0.01). It was then concluded that shear stress can induce endothelial NO synthesis and caveolae plays a key role in shear stress-induced eNOS activation.
Li
Yinping
Y
Faculty of Pathology and Pathophysiology, Wuhan University, Wuhan 430071, China.
Ouyang
Jingping
J
Zheng
Hanqiao
H
Yu
Zhui
Z
Wang
Baohua
B
D021941:Caveolae / Q000502:physiology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D005372:Filipin / Q000494:pharmacology; D006801:Humans; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D033081:Shear Strength; D014471:Umbilical Veins / Q000166:cytology
D005372:Filipin; D052250:Nitric Oxide Synthase Type III