[Lyophilization for platelet preservation].
To explore a new lyophilized preservation methods for human platelets, platelets were pre-treated with aldehyde, human albumin or trehalose was added to the system of condensed cooling as protectant to stabilize the structure of platelets. The optimal resuspending buffer was also selected in the study. The morphological changes of platelets were observed by using electron microscopy after lyophilization, and the expression of membrane proteins on platelets was detected also after lyophilization. The results indicated that the recovery rate of platelets treated with aldehyde was generally more than 60%. Aggregative ability was reduced a little than the platelet untreated. 5% of human albumin had an advantage over 40 mmol/L of trehalose in respect of the preservation effect. In the way of keeping aggregative ability, PPP was obviously better than PBS. The results of electron microscopy displayed that organelles including mitochondria and excreted granules could be observed distinctly. Whereas, expression of membrane proteins of platelet treated with aldehyde was evidently dropped as compared with those of the fresh platelet. In conclusion, aldehyde as a novel protective agent, has excellent effects on lyophilization of platelets and is worthy to be further studied.
Cao
Wei
W
Beijing Institute of Transfusion Medicine, Beijing 100850, China.
Wang
Yan
Y
Jing
Peng
P
Liu
An
A
Pei
Hai-Yun
HY
Zhu
Rui-Quan
RQ
Ye
Chu-Yan
CY
Han
Ying
Y
D000418:Albumins / Q000494:pharmacology; D000447:Aldehydes / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects; D001793:Blood Preservation / Q000379:methods*; D005612:Freeze Drying; D006801:Humans; D015203:Reproducibility of Results; D014199:Trehalose / Q000494:pharmacology
D000418:Albumins; D000447:Aldehydes; D014199:Trehalose