title: Autoradiographic localization and binding study of benzodiazepines receptor sites in carp brain (Cyprinus carpio L.).
abstract: This study demonstrates, for the first time, by both autoradiography and binding assay that [3H]Ro 15-1788 binds to carp brain with a high degree of anatomical selectivity. Saturation binding of the radioligand was determined in seven anatomically defined regions and suggested the presence of one class of binding sites (Type I-lke). In general, there was a good correlation between the autoradiographic and the binding data. By far, the optic tectum and the vagal, facial, and glossopharyngeal lobes showed the majority of [3H]Ro 15-1788 binding sites. Low to negative concentration of binding sites was detected in the cerebellum. The location of [3H]Ro 15-1788 binding sites in particular brain regions, indicates that benzodiazepine receptors could be associated with pathways involved in the control of basic central functions as spatial learning acquisition and retention, and feeding behaviour.
authors.affiliation: Department of Animal Productions, Section of Anatomy, University of Pisa, Via Matteotti 5, 56100 Pisa, Italy. apirone@vet.unipi.it
authors.forename: Andrea
authors.initials: A
authors.lastname: Pirone
authors.forename: Gino
authors.initials: G
authors.lastname: Giannaccini
authors.forename: Laura
authors.initials: L
authors.lastname: Betti
authors.forename: Antonio
authors.initials: A
authors.lastname: Lucacchini
authors.forename: Giovanni
authors.initials: G
authors.lastname: Mascia
authors.forename: Laura
authors.initials: L
authors.lastname: Fabbrini
authors.forename: Paola
authors.initials: P
authors.lastname: Italiani
authors.forename: Angela
authors.initials: A
authors.lastname: Uccelli
authors.forename: Carla
authors.initials: C
authors.lastname: Lenzi
authors.forename: Ortenzio
authors.initials: O
authors.lastname: Fabiani
mesh_terms: D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry / Q000502:physiology*; D002347:Carps / Q000378:metabolism*; D003627:Data Interpretation, Statistical; D005442:Flumazenil / Q000378:metabolism; D018757:GABA Modulators / Q000378:metabolism; D058785:GABA-A Receptor Agonists; D058787:GABA-A Receptor Antagonists; D008566:Membranes / Q000378:metabolism; D011963:Receptors, GABA-A / Q000378:metabolism*; D013649:Taste / Q000502:physiology
chemical_list: D018757:GABA Modulators; D058785:GABA-A Receptor Agonists; D058787:GABA-A Receptor Antagonists; D011963:Receptors, GABA-A; D005442:Flumazenil