title: Identification of asymmetrically localized transcripts along the animal-vegetal axis of the Xenopus egg.
abstract: In many organisms, proper embryo development depends on the asymmetrical distribution of mRNA in the cytoplasm of the egg. Here we report comprehensive screening of RNA localized in the animal or vegetal hemisphere of the Xenopus egg. Macroarrays including over 40,000 independent embryonic cDNA clones, representing at least 17,000 unigenes, were differentially hybridized with labeled probes synthesized from the mRNA of animal or vegetal blastomeres. After two rounds of screening, we identified 33 clones of transcripts that may be preferentially distributed in the vegetal region of the early stage embryo, but transcripts localized in the animal region were not found. To assess the array results, we performed northern blot and quantitative real-time reverse transcription-polymerase chain reaction analysis. As a result, 21 transcripts of the 33 were confirmed to be localized in the vegetal region of the early stage embryo. Whole-mount in situ hybridization analysis revealed that 11 transcripts, including 7 previously reported genes, were localized in the vegetal hemisphere of the egg. These 11 transcripts were categorized into three groups according to their expression patterns in the egg. The first group, which contained four transcripts, showed uniform expression in the vegetal hemisphere, similar to VegT. The second group, which contained three transcripts, showed gradual expression from the vegetal pole to the equator, similar to Vg1. The last group, which contained three transcripts, was expressed at the germ plasm, similar to Xdazl. One transcript, Xwnt11, showed both the second and the third expression patterns.
authors.affiliation: Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Akou 678-1297, Japan. r104y005@stkt.u-hyogo.ac.jp
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kataoka
authors.forename: Akira
authors.initials: A
authors.lastname: Tazaki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kitayama
authors.forename: Naoto
authors.initials: N
authors.lastname: Ueno
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Makoto
authors.initials: M
authors.lastname: Mochii
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000502:physiology; D004625:Embryo, Nonmammalian; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D010063:Ovum / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D012333:RNA, Messenger