Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis: diagnosis of cutaneous leishmaniasis by polymerase chain reaction.
An evaluation of 5 laboratory methods for diagnosing American cutaneous leishmaniasis (ACL) was carried out on patients from an endemic area of Brazil. From 164 patients presenting cutaneous lesions, and suspected to have ACL, 133 (81.1%) were confirmed for the disease by Montenegro skin test (MST) and/or parasitologic examination (PE). In both groups of patients, the positivity of polymerase chain reaction (PCR) was similar to that of immunofluorescence assay and enzyme-linked immunosorbent assay, and higher than that of MST and PE (P < .05). In the group of patients suspected to have ACL, PCR presented the same positivity as PE and MST together. No correlation between positivity of the laboratory methods and clinical or epidemiologic aspects was observed. Our data confirmed the value of PCR as an alternative laboratory method for diagnosing ACL, especially for those patients with negative PE and MST.
Marques
Marcos J
MJ
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil.
Volpini
Angela C
AC
Machado-Coelho
George L L
GL
Machado-Pinto
Jackson
J
da Costa
Carlos A
CA
Mayrink
Wilson
W
Genaro
Odair
O
Romanha
Alvaro J
AJ
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001938:Brazil; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis*; D006801:Humans; D007891:Leishmania / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D016133:Polymerase Chain Reaction
D000913:Antibodies, Protozoan; D017931:DNA Primers; D016054:DNA, Protozoan