title: Peritubular capillary damage in acute humoral rejection: an ultrastructural study on human renal allografts.
abstract: The ultrastructural features of peritubular capillary (PC) damage was studied in 12 kidney allografts with acute humoral rejection (AHR). AHR manifested in diffuse linear PC staining for C4d, and histology consistent with Banff grade III in 7 recipients and Banff grade II in 5. Allografts with acute tubular necrosis served as controls. First biopsies (post-transplantation day 16.2 +/- 2.2): The intra-capillary exudate comprised monocytes (59%), polymorphonuclears (14%), lymphocytes (12%) and not otherwise specified mononuclears (15%). Three patterns of focal PC endothelial injury were observed: lysis, an increased rate of apoptosis and fragmentation. No correlation was found between the respective damage types and the inflammatory cell types or the Banff grades. Controls revealed endothelial swelling, detachment from basement membrane and fragmentation. Follow-up biopsies: Monocytes transformed into macrophages intra-luminally. The reparative changes comprised endothelial cytoplasmic protrusions, binucleated endothelial cells and capillary sprouts. Early transplant capillaropathy and transplant glomerulopathy were noted in 2 recipients. Literature data indicate that lysis is mediated by anti-HLA alloantibodies; apoptosis, demonstrated first in the present study, may be induced by non-HLA-type anti-endothelial antibodies. Fragmentation is caused by ischemia. Ongoing endothelial injury leads to transplant capillaropathy and transplant glomerulopathy, the characteristic lesions of chronic rejection.
authors.affiliation: Department of Pathology, University of Szeged, Szeged, Hungary. lippeti@yahoo.co.uk
authors.forename: P
authors.initials: P
authors.lastname: Lipták
authors.forename: E
authors.initials: E
authors.lastname: Kemény
authors.forename: Z
authors.initials: Z
authors.lastname: Morvay
authors.forename: E
authors.initials: E
authors.lastname: Szederkényi
authors.forename: P
authors.initials: P
authors.lastname: Szenohradszky
authors.forename: F
authors.initials: F
authors.lastname: Marofka
authors.forename: J
authors.initials: J
authors.lastname: Toldi
authors.forename: M
authors.initials: M
authors.lastname: Exner
authors.forename: B
authors.initials: B
authors.lastname: Iványi
mesh_terms: D000208:Acute Disease; D000917:Antibody Formation; D001485:Basement Membrane / Q000473:pathology / Q000648:ultrastructure; D001706:Biopsy; D002908:Chronic Disease; D004730:Endothelium, Vascular / Q000473:pathology* / Q000648:ultrastructure; D006084:Graft Rejection / Q000276:immunology* / Q000473:pathology*; D006801:Humans; D007678:Kidney Glomerulus / Q000473:pathology / Q000648:ultrastructure; D016030:Kidney Transplantation; D008264:Macrophages / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron; D014184:Transplantation, Homologous