title: Adherence of Acanthamoeba to human corneal epithelial cells recovered from normal non-lens wearers and asymptomatic contact lens wearers.
abstract: To increase our knowledge of factors leading to Acanthamoeba keratitis in contact lens wearers, we determined the ability of this organism to adhere to corneal epithelial cells (EC) recovered from non-lens wearers (NL) and from subjects using hydrogel contact lenses on a daily (DW) and extended wear (EW) schedule. ECs were incubated with trophozoites of Acanthamoeba and, after 3 h, the median per cent of cells exhibiting adherence was 24, 23 and 23 for NL, DW and EW groups respectively (P=0.552, Kruskal-Wallis Test). There were no differences between the groups for the number of adherent amoebae and a significant majority had only one adherent trophozoite per EC. No difference in adherence was seen with increasing exposure time. Factors other than amoebic adherence to superficial corneal EC are responsible for the increased incidence of Acanthamoeba keratitis in lens wearers.
authors.affiliation: L.V. Prasad Eye Institute, Hyderabad, India. savitri@Ivpeye.stph.net
authors.forename: S
authors.initials: S
authors.lastname: Sharma
authors.forename: P R
authors.initials: PR
authors.lastname: Sankaridurg
authors.forename: N
authors.initials: N
authors.lastname: Venkata
authors.forename: S
authors.initials: S
authors.lastname: Potluri
authors.forename: S
authors.initials: S
authors.lastname: Athmanathan
authors.forename: S
authors.initials: S
authors.lastname: Reddy
authors.forename: B A
authors.initials: BA
authors.lastname: Holden
authors.forename: G N
authors.initials: GN
authors.lastname: Rao