The effect of colloidal fluid replacement on wound healing in an experimental sublethal hemorrhagic shock model.
OBJECTIVE
The aim of the study was to evaluate the effects of different resuscitation solutions on wound healing in an animal model of sublethal hemorrhagic shock.


METHODS
Sublethal hemorrhagic shock was established by blood withdrawal until the mean arterial pressure fell to 40 mmHg within 15 min. Animals were resuscitated with four different solutions: group I (n=19) 0.9% NaCl, group II (n=20) 10% dextran 40, group III (n=20) 6% hydroxyethyl starch, group IV (n=18) 4% modified fluid gelatin. Following inhalation anesthesia, a full thickness skin incision of 2 cm in length was made on the abdominal wall and sutured primarily. The incision wound on the abdominal wall was excised on the fifth day. The breaking strength of the incision and hydroxyproline content of dry tissue were evaluated.


RESULTS
Differences in breaking strength values were observed between groups I-III and I-IV (P<0.05). Hydroxyproline values were significantly lower in group IV (58.47 microg/ml) and group II (69.17 microg/ml) (P<0.05). The gelatin-resuscitated group had lower breaking strength and hydroxyproline values than the other groups.


CONCLUSIONS
It can be concluded that resuscitation with modified fluid gelatin may have detrimental effects on wound healing in an experimental model of sublethal hemorrhagic shock.
Eroglu
Erol
E
Department of General Surgery, Suleyman Demirel University, School of Medicine, Isparta, Turkey. heroleroglu@hotmail.com
Eroglu
Fusun
F
Yavuz
Lutfi
L
Agalar
Canan
C
Agalar
Fatih
F
D000818:Animals; D003102:Colloids / Q000627:therapeutic use*; D005440:Fluid Therapy / Q000379:methods*; D006909:Hydroxyproline / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000628:therapy*; D014945:Wound Healing / Q000187:drug effects*
D003102:Colloids; D006909:Hydroxyproline