title: Seeligeriolysin O, a protein toxin of Listeria seeligeri, stimulates macrophage cytokine production via Toll-like receptors in a profile different from that induced by other bacterial ligands.
abstract: Seeligeriolysin O (LSO), a member of cholesterol-dependent cytolysins of Listeria seeligeri, exhibits cytokine-inducing activity. In this study, we examined the profile of cytokines expressed in macrophages of mice after stimulation with full-length form of recombinant LSO (rLSO530), C-terminal-truncated protein (rLSO483) and two authentic cytokine-inducing Toll-like receptor (TLR) ligands from bacteria, peptidoglycan (PGN) and LPS. Both rLSO530 and rLSO483 were able to induce IL-12 p40 and IL-12 p70 more strongly in macrophages than PGN or LPS. In contrast, IFN-beta and nitric oxide were induced by LPS but not by rLSO530, rLSO483 or PGN. In the presence of exogenously added IFN-beta, IL-12 p40 and IL-12 p70 production was inhibited after LSO stimulation, but IL-12 p70 production was enhanced after PGN stimulation. Although LSO signaling appeared to be associated with both TLR2 and TLR4, the profile of cytokine production by LSO stimulation was distinct from those by stimulation with PGN or LPS. Thus, it was shown that LSO is a unique bacterial ligand that induces macrophage cytokine production in a manner different from PGN or LPS.
authors.affiliation: Department of Microbiology, Kyoto University Graduate School of Medicine, Japan. yutaka@kuhp.kyoto-u.ac.jp
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ito
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kawamura
authors.forename: Chikara
authors.initials: C
authors.lastname: Kohda
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: Takamasa
authors.initials: T
authors.lastname: Nomura
authors.forename: Masao
authors.initials: M
authors.lastname: Mitsuyama
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology* / Q000494:pharmacology; D001427:Bacterial Toxins / Q000276:immunology* / Q000494:pharmacology; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008087:Listeria / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000276:immunology; D010457:Peptidoglycan / Q000276:immunology / Q000494:pharmacology; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000172:deficiency / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D016207:Cytokines; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D010457:Peptidoglycan; D052899:Pore Forming Cytotoxic Proteins; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; C510990:seeligeriolysin O, Listeria seeligeri; D054353:Perforin; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse