title: Studies on the interaction of Nile red with horseradish peroxidase in solution.
abstract: The interaction of an extrinsic probe (Nile red) with an enzyme (horseradish peroxidase) in solution was investigated using fluorescence techniques. Nile red fluorescence is very environmentally sensitive and the presence of domains of differing polarity within the enzyme was ascertained by the decomposition of the Nile red emission spectrum. Further evidence for the position of the probe inside the enzyme was obtained from a molecular modeling study. A decrease in the emission intensity of the dye during incubation with horseradish peroxidase was explained by the occurrence of resonance energy transfer between the Nile red and the heme group in the enzyme. This was supported by a calculation of the critical transfer distance and a comparison of the fluorescence intensity of the dye in both the holo- and apo-enzyme. These data were then applied to the study of the effect of temperature on the structure of the enzyme, where changes in conformation were elucidated.
authors.affiliation: Departamento de Física, Universidade do Minho, Braga, Portugal. graham@fisica.uminho.pt
authors.forename: Graham
authors.initials: G
authors.lastname: Hungerford
authors.forename: Ana
authors.initials: A
authors.lastname: Rei
authors.forename: M Isabel C
authors.initials: MI
authors.lastname: Ferreira
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006418:Heme / Q000737:chemistry; D020035:Holoenzymes / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D010078:Oxazines / Q000737:chemistry*; D011487:Protein Conformation; D013696:Temperature
chemical_list: D005456:Fluorescent Dyes; D020035:Holoenzymes; D010078:Oxazines; D006418:Heme; D006735:Horseradish Peroxidase; C044808:nile red