title: A novel method to obtain microtensile specimens minimizing cut flaws.
abstract: Cutting procedures conventionally used for microtensile specimens' preparation induce mechanical stress and attrition, leading to cracks in dental structure. Enamel is mainly affected due to its high module of elasticity and brittleness. As an alternative to the conventional diamond saw, a diamond wire was used for cutting. The two techniques were compared in efficacy in the preparation of two different sample shapes. Forty human molars, flattened in dentin or enamel, were used for sample preparation. After bonding procedures, resin block was built incrementally. Half of the teeth were cut using a diamond disc and the other half using a diamond wire, obtaining slabs and stick-shaped specimens. Slabs were then shaped to obtain hourglass samples. Five samples from each subgroup were randomly selected for Scanning electron microscopy (SEM) analysis. Remaining samples underwent microtensile test and the results were analyzed, including/excluding premature failures (pf), with three-way ANOVA and Tukey test, performed separately, by substrate. On dentin, only the exclusion of pf showed a significant higher bond strength values than when pf were included (p < 0.05); however, on enamel, all factors (equipment, shape and including/excluding pf) had a significant effect on bond strength (p<0.05). The most remarkable finding from SEM was the incidence of specimens' cracks and defects when diamond blade was used. Scratches and cracks in the hourglass-shaped specimens were also detected. In both cases, enamel was more affected than dentin.
authors.affiliation: Department of Dental Materials, University of São Paulo, São Paulo, Brazil. fesadek@usp.br
authors.forename: Fernanda Tranchesi
authors.initials: FT
authors.lastname: Sadek
authors.forename: Francesca
authors.initials: F
authors.lastname: Monticelli
authors.forename: Antonio
authors.initials: A
authors.lastname: Muench
authors.forename: Marco
authors.initials: M
authors.lastname: Ferrari
authors.forename: Paulo Eduardo Capel
authors.initials: PE
authors.lastname: Cardoso
mesh_terms: D003743:Dental Enamel; D003804:Dentin; D018130:Diamond; D006801:Humans; D008963:Molar; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods*
chemical_list: D018130:Diamond