Bio-sensing on a chip with compact discs and nanofibers.
This paper describes a novel, sensitive detection system for biomolecules (DNA and proteins etc.) that is integrated in a lab-on-a-chip utilizing optical compact discs (CDs) and bio-nanofibers. The new method comprises a microchannel containing CD grating that confines fragments of unique bacterial cellulose fibrils (BC), which have nanometre scale fibers and holes. A maximum of six times higher sensitivity to detect DNA was obtained with this CD and BC system compared to a conventional method. We also demonstrate an effective light-confining effect for biological application with the new method.
Tabuchi
Mari
M
Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Science, The University of Tokushima COE, 1-78 Shomachi, Tokushima 770-8505, Japan. tabuchi@ph.tokushima-u.ac.jp
Kobayashi
Kazuyoshi
K
Fujimoto
Masayuki
M
Baba
Yoshinobu
Y
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D016250:Compact Disks; D004247:DNA / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007834:Lasers; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods
D020099:Coated Materials, Biocompatible; D004247:DNA