title: Efficacy of cetylpyridinium chloride against Listeria monocytogenes and its influence on color and texture of cooked roast beef.
abstract: Sliced (cut) and exterior (intact) surfaces of restructured cooked roast beef were inoculated with Listeria monocytogenes, treated with cetylpyridinium chloride (CPC; immersion in 500 ml of 1% solution for 1 min), individually vacuum packaged, and stored for 42 days at 0 or 4 degrees C. Noninoculated samples were similarly treated, packaged, and stored to determine effects on quality (color and firmness) and on naturally occurring bacterial populations, including aerobic plate counts and lactic acid bacteria. Immediately after CPC treatment, regardless of inoculation level, L. monocytogenes populations were reduced (P = 0.05) by about 2 log CFU/cm2 on sliced surfaces and by about 4 log CFU/cm2 on exterior surfaces. Throughout 42 days of refrigerated storage (at both 0 and 4 degrees C), L. monocytogenes populations on CPC-treated samples remained lower (P = 0.05) than those of nontreated samples for both surface types. After 42 days of storage at both 0 and 4 degrees C, aerobic plate count and lactic acid bacteria populations of treated samples were 1 to 1.5 log CFU/cm2 lower (P = 0.05) than those of nontreated samples for both surface types. CPC treatment resulted in negligible effects (P > 0.05) on the color (L*, a*, and b* values) of exterior and sliced roast beef surfaces during storage. For both sliced and exterior surfaces, CPC-treated samples were generally less firm than nontreated samples. CPC treatment effectively reduced L. monocytogenes populations on roast beef surfaces and resulted in relatively minor impacts on color and texture attributes. CPC treatment, especially when applied to products prior to slicing, may serve as an effective antimicrobial intervention for ready-to-eat meat products.
authors.affiliation: Department of Animal Sciences & Industry and Food Science Institute, Kansas State University, Manhattan, Kansas 66506, USA.
authors.forename: M
authors.initials: M
authors.lastname: Singh
authors.forename: H
authors.initials: H
authors.lastname: Thippareddi
authors.forename: R K
authors.initials: RK
authors.lastname: Phebus
authors.forename: J L
authors.initials: JL
authors.lastname: Marsden
authors.forename: T J
authors.initials: TJ
authors.lastname: Herald
authors.forename: A L
authors.initials: AL
authors.lastname: Nutsch
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002417:Cattle; D002594:Cetylpyridinium / Q000494:pharmacology*; D015169:Colony Count, Microbial; D005511:Food Handling / Q000379:methods; D018857:Food Packaging / Q000379:methods; D005519:Food Preservation / Q000379:methods*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008461:Meat Products / Q000032:analysis* / Q000592:standards; D010858:Pigmentation; D013696:Temperature; D013997:Time Factors; D014618:Vacuum
chemical_list: D000891:Anti-Infective Agents, Local; D002594:Cetylpyridinium