title: Surface modification to reduce nonspecific binding of quantum dots in live cell assays.
abstract: Nonspecific binding is a frequently encountered problem with fluorescent labeling of tissue cultures when labeled with quantum dots. In these studies various cell lines were examined for nonspecific binding. Evidence suggests that nonspecific binding is related to cell type and may be significantly reduced by functionalizing quantum dots with poly(ethylene glycol) ligands (PEG). The length of PEG required to give a significant reduction in nonspecific binding may be as short as 12-14 ethylene glycol units.
authors.affiliation: Department of Chemistry, Vanderbilt University, Station B, 351822, Nashville, Tennessee 37235-1822, USA.
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Bentzen
authors.forename: Ian D
authors.initials: ID
authors.lastname: Tomlinson
authors.forename: John
authors.initials: J
authors.lastname: Mason
authors.forename: Paul
authors.initials: P
authors.lastname: Gresch
authors.forename: Michael R
authors.initials: MR
authors.lastname: Warnement
authors.forename: David
authors.initials: D
authors.lastname: Wright
authors.forename: Elaine
authors.initials: E
authors.lastname: Sanders-Bush
authors.forename: Randy
authors.initials: R
authors.lastname: Blakely
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: Rosenthal
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D003584:Cytological Techniques; D005456:Fluorescent Dyes / Q000592:standards*; D011092:Polyethylene Glycols / Q000494:pharmacology; D045663:Quantum Dots; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D005456:Fluorescent Dyes; D011092:Polyethylene Glycols; C006903:carbopol 940