title: Surface modification of poly(ethylene terephthalate) via hydrolysis and layer-by-layer assembly of chitosan and chondroitin sulfate to construct cytocompatible layer for human endothelial cells.
abstract: Surface modification of poly(ethylene terephthalate) (PET) film was performed by surface hydrolysis and layer-by-layer (LBL) assembly followed a mechanism of electrostatic adsorption of oppositely charged polymers, exemplified with chitosan and chondroitin sulfate (CS). Hydrolysis of PET in concentrated alkaline solution produced a carboxyl-enriched surface. The changes of weight loss and surface chemistry, morphology and wettability were monitored and verified by UV-vis spectroscopy, atomic force microscopy (AFM) and water contact angle. Assembly of positively charged chitosan and negatively charged CS was then conducted in a LBL manner to create multilayers on the hydrolyzed PET film. The process of layer growth and oscillation of surface wettability were monitored by UV-vis spectroscopy and water contact angle measurement, respectively. In vitro cell culture revealed that the adherence of endothelial cells was significantly enhanced on the biomacromolecules-modified PET film with preserved endothelial cell function, in particular on those assembled with larger number of chitosan/CS layers. However, with regard to cell proliferation and viability properties after cultured for 4 days, minor difference was determined between the modified and the unmodified PET films.
authors.affiliation: Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Yunxiao
authors.initials: Y
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: He
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D002809:Chondroitin Sulfates / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007231:Infant, Newborn; D018625:Microscopy, Atomic Force; D011092:Polyethylene Glycols / Q000737:chemistry*; D011093:Polyethylene Terephthalates; D013499:Surface Properties; D014471:Umbilical Veins / Q000166:cytology; D017155:Wettability; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D011093:Polyethylene Terephthalates; D014841:von Willebrand Factor; D011092:Polyethylene Glycols; D002809:Chondroitin Sulfates; D048271:Chitosan