Inhibition of p38 mitogen-activated protein kinase protects human islets from cryoinjury and improves the yield, viability, and quality of frozen-thawed islets.
The development of an optimal islet cryopreservation method will permit transplantation of islets from multiple donors in a single procedure and contribute to alleviation of the islet shortage. In this study, we have improved human islet cryopreservation methods under serum-free conditions using an intracellular-based islet cryopreservation solution (ICS), especially supplemented with a p38 pathway inhibitor (p38IH) to suppress p38 mitogen-activated protein kinase (MAPK) activation. Three different solutions were compared for freezing and thawing of human islets (1) conventional RPMI1640 medium, (2) ICS, and (3) ICS supplemented with a p38IH, SD-282 (ICS-p38IH). Islet cryopreservation with ICS-p38IH significantly improved islet recovery, viability, and quality after thawing of cryopreserved islets. This improvement may allow the use of cryopreserved islets in clinical islet transplantation.
Omori
K
K
Department of Diabetes, Endocrinology and Metabolism, City of Hope National Medical Center/Beckman Research Institute, 1500 East Duarte Road, Duarte, CA 91010, USA.
Valiente
L
L
Orr
C
C
Rawson
J
J
Ferreri
K
K
Todorov
I
I
Medicherla
S
S
Protter
A A
AA
Schreiner
G F
GF
Riggs
A D
AD
Kandeel
F
F
Mullen
Y
Y
D018929:Cell Culture Techniques; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D004789:Enzyme Activation; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D048051:p38 Mitogen-Activated Protein Kinases