Efficient spin-spin scalar coupling mediated C-13 homonuclear polarization transfer in biological solids without proton decoupling.
We demonstrate that an efficient C' <--> C alpha polarization transfer based on J-coupling can be realized under fast magic-angle spinning (MAS) condition without 1H decoupling. Experimental results are presented for model crystalline compounds as well as a non-crystalline 17-residue polypeptide MB(i + 4)EK. Measurements on MB(i + 4)EK demonstrate that 53% of the initial C' polarization was transferred to the cross peaks at 7.05 T under 25 kHz MAS spinning.
Mou
Yun
Y
Department of Chemistry, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei, Taiwan.
Chao
John Chin Hao
JC
Chan
Jerry C C
JC
D000409:Alanine / Q000032:analysis*; D001704:Biopolymers / Q000032:analysis*; D002247:Carbon Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D011522:Protons; D013113:Spin Labels; D014633:Valine / Q000032:analysis*
D001704:Biopolymers; D002247:Carbon Isotopes; D010455:Peptides; D011522:Protons; D013113:Spin Labels; D014633:Valine; D000409:Alanine