Shewanella pneumatophori sp. nov., an eicosapentaenoic acid-producing marine bacterium isolated from the intestines of Pacific mackerel (Pneumatophorus japonicus).
An eicosapentaenoic acid-producing bacterium, previously described as Shewanella sp. strain SCRC-2738, was classified by phenotypic characterization, chemotaxonomic analysis, 16S rRNA gene sequence analysis and DNA-DNA hybridization. The isolate was Gram-negative, rod-shaped and motile by using polar flagella. The strain grew at 4-32 degrees C; the optimum growth temperature was 27 degrees C. NaCl was required for growth. The major isoprenoid quinones were ubiquinone-7 and ubiquinone-8 and its DNA G+C content was 42.8 mol%. The whole-cell fatty acids mainly (above 5 %) consisted of iso-C(13 : 0), iso-C(15 : 0), C(16 : 0), C(16 : 1)omega7c, C(18 : 1)omega7c and C(20 : 5)omega3 (eicosapentaenoic acid). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain SCRC-2738T was related closely (sequence similarities above 99 %) to Shewanella marinintestina (99.3 %), Shewanella sairae (99.3 %) and Shewanella schlegeliana (99.2 %). DNA-DNA hybridization and phenotypic characteristics confirmed that strain SCRC-2738T merited classification as a novel species of the genus Shewanella, for which the name Shewanella pneumatophori sp. nov. is proposed. The type strain is SCRC-2738T (=JCM 13187T=NCIMB 14060T).
Hirota
Kikue
K
Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Nodasaka
Yoshinobu
Y
Laboratory of Electron Microscopy, Graduate School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan.
Orikasa
Yoshitake
Y
Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan.
Okuyama
Hidetoshi
H
Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan.
Yumoto
Isao
I
Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.|Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D005227:Fatty Acids / Q000032:analysis; D007422:Intestines / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010473:Perciformes / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis* / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D020592:Shewanella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D015118:Eicosapentaenoic Acid