Regulatory role of Na,K-ATPase in the growth of heart tissue explants in organotypic culture.
We studied the effects of ouabain, norepinephrine, and acetylcholine on the growth of heart tissue explants from 10-12-old chicken embryos in an organotypic culture. Ouabain, norepinephrine, and acetylcholine stimulated the growth of explants. Our results suggest that trophic function of acetylcholine and norepinephrine is associated with regulation of Na,K-ATPase activity as a signal transducer.
Lopatina
E V
EV
V. A. Almazov Institute of Cardiology, Russian Ministry of Health, St. Petersburg. evl1112@infran.ru
Penniyainen
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VA
Zaika
A A
AA
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation; D006321:Heart / Q000033:anatomy & histology*; D009206:Myocardium / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D009924:Organ Culture Techniques / Q000379:methods*; D010042:Ouabain / Q000494:pharmacology; D015398:Signal Transduction; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry* / Q000502:physiology*
D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D000109:Acetylcholine; D009638:Norepinephrine