[Effect of polyphenols from the Mediterranean diet on proliferation and mediators of in vitro invasiveness of the MB-49 murine bladder cancer cell line].
OBJECTIVE
To evaluate the effect of different polyphenols on the proliferation and invasive capacity of MB-49 murine bladder tumor cell lines and to identify the mediators involved in this process.


METHODS
MB-49 murine bladder cancer cells were cultured in media supplemented with resveratrol, rutin, morin, quercetin, gallic acid and tannic acid (all of them are polyphenols usually present in Mediterranean diet) for periods of 24, 48 and 72 hours to quantify the expression of urokinase-type plasminogen activator (uPA) and its receptor (uPAR) in the culture medium, as well as of metalloproteinase-9 (MMP-9) and cell proliferation.


RESULTS
All the polyphenols studied significantly inhibited proliferation of MB-49 cells, varying according to the time periods and doses used. The cells in the media supplemented with the nutrients to study did not show inhibition of mRNA expression of urokinase-type plasminogen activator (uPA) or its high affinity receptor (uPAR). It was even slightly increased in certain cases. However, mRNA expression of metalloproteinase-9 was strongly inhibited.


CONCLUSIONS
The polyphenols present in our usual diet exert an effect on the proliferation and mediators of bladder tumor invasiveness in MB-49 cells.
García Mediero
J M
JM
Servicio de Urologia, Hospital Universitario de Getafe, Madrid. garciamediero@hotmail.com
Ferruelo Alonso
A
A
Páez Borda
A
A
Luján Galán
M
M
Angulo Cuesta
J
J
Chiva Robles
V
V
Berenguer Sánchez
A
A
D000818:Animals; D045744:Cell Line, Tumor / Q000737:chemistry / Q000187:drug effects / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D038441:Diet, Mediterranean; D005419:Flavonoids / Q000494:pharmacology*; D066298:In Vitro Techniques; D020782:Matrix Metalloproteinases / Q000502:physiology; D009361:Neoplasm Invasiveness / Q000473:pathology*; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000178:diet therapy / Q000473:pathology*
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D020782:Matrix Metalloproteinases