Sero-molecular evaluation of human cytomegalovirus disease in renal transplant rejection.
Cytomegalovirus (CMV) is the most common viral pathogen in renal transplant recipients resulting in graft rejection. The prevalence of CMV disease and renal graft rejection is not well studied in India. Sequential specimens from 32 renal allograft recipients were examined by using CMV IgM specific mu capture ELISA and DNA by PCR. Twelve of the 32 patients were CMV IgM positive and out of 12 patients, 9 had rejection and 4 experienced CMV disease. CMV IgM specific mu capture ELISA helped in diagnosis of CMV disease, though it is less sensitive in detection of rejection. PCR itself was proved not sensitive enough in detecting either CMV disease or rejection. At present, optimal laboratory detection of CMV infection in these patients can be achieved only by multiple and more sensitive parameters.
Kishore
Janak
J
Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow-226014, India. janak@sgpgi.ac.in
Mukhopadhyay
Chiranjoy
C
Savitri
Ayyagari
Archana
A
Sharma
Rakesh Kumar
RK
D000328:Adult; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D016021:Epidemiologic Studies; D005260:Female; D006084:Graft Rejection / Q000821:virology*; D006801:Humans; D007194:India / Q000453:epidemiology; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence; D018570:Risk Assessment; D012307:Risk Factors