[Expression of antigenic region of VP1 gene of swine vesicular disease in Escherichia coli].
The antigenic region of VP1 gene of swine vesicular disease virus was amplified by reverse transcription polymerase chain reaction (RT-PCR) and nested polymerase chain reaction (nPCR). After the amplified fragment was cloned into the expression vector pProEX-HTb. The insert position,the size and the reading frame of the insertion were identified by PCR, restriction digestion and sequence analysis of the recombinant plasmids. SDS-PAGE and Western blot indicated that the transformed BL21(DE3) by the recombinant plasmids and induced by IPTG could express the antigen region of VP1 of swine vesicular disease virus, the expressed antigen protein could be recognized by the positive serum of SVDV.
Zhang
Yongguo
Y
Lanzhou Veterinary Research Institute, Chinese Agriculture Academy of Sciences, Lanzhou 730046, China.
Liu
Xiangtao
X
Han
Xueqing
X
Zhang
Yanming
Y
Xie
Qingge
Q
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D029822:Enterovirus B, Human / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D013555:Swine Vesicular Disease / Q000276:immunology / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
D000956:Antigens, Viral; D014764:Viral Proteins