Suppressive effect of Clostridium perfringens-produced heat-stable substance(s) on proliferation of human colon adenocarcinoma HT29 cells in culture.
Clostridium perfringens has been regarded as one of the intestinal bacteria increasing colon cancer risk. In previous studies, we have shown that the oral administration of C. perfringens culture medium can inhibit the mutagen-induced formation of pre-neoplastic lesions in rat colon, thus proposing the existence of factor(s) preventing colon tumorigenesis in this culture medium. However, the properties of effective factor(s) and the mechanism of this inhibitory action still remain to be investigated. Then, the effect of C. perfringens culture medium on human colon adenocarcinoma HT29 cells was examined. The exposure of HT29 cells to C. perfringens culture medium was found to suppress the proliferation of these cells probably through the reduction of immediate early gene egr-1 expression. These observations suggest that C. perfringens culture medium has a cytostatic action on colon tumor cells, which may be responsible for the prevention of pre-neoplastic formation in rat colon.
Arimochi
Hideki
H
Department of Molecular Bacteriology, Graduate School of Medicine, The University of Tokushima, Kuramoto, Tokushima 770-8503, Japan. arimochi@basic.med.tokushima-u.ac.jp
Morita
Kyoji
K
Kataoka
Keiko
K
Nakanishi
Shusuke
S
Kuwahara
Tomomi
T
Ohnishi
Yoshinari
Y
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D003015:Clostridium Infections; D003016:Clostridium perfringens / Q000502:physiology*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D003470:Culture Media / Q000494:pharmacology*; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D019073:HT29 Cells; D006358:Hot Temperature; D006801:Humans; D015151:Immunoblotting; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology
D003470:Culture Media; D051766:Early Growth Response Protein 1