Modulation of beta-actin, insulin-like growth factor 1 and glucose-6-phosphate dehydrogenase gene expression during vibriosis of sea bream, Sparus (=Rhabdosargus) sarba Forsskål.
Gene expression changes, related to growth regulation, were investigated in silver sea bream, Sparus (=Rhabdosargus) sarba, subjected to vibriosis. Using reverse transcriptase polymerase chain reaction assays, transcript levels of beta-actin, insulin-like growth factor 1 (IGF-1) and glucose-6-phosphate dehydrogenase (G6PDH) were analysed. A tissue-specific response for beta-actin was found, as transcript levels in the kidney increased during early- and mid-stage vibriosis, but remained unchanged in liver tissue. The gene transcript levels of IGF-1 were found to decrease in both liver and kidney from an early stage of vibriosis. The gene transcript levels of G6PDH increased at early and mid stages of vibriosis in liver and kidney before decreasing during the late stage of vibriosis. Further studies on G6PDH demonstrated that chemical inhibition of G6PDH in silver sea bream fibroblasts resulted in increased cell death upon exposure to hydrogen peroxide. The results from the present study demonstrate the complexity of growth-related gene expression events that occur in fish tissues during disease.
Deane
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Department of Biology, The Chinese University of Hong Kong, Shatin, NT, China.
Woo
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D000199:Actins / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D017931:DNA Primers; D005393:Fish Diseases / Q000378:metabolism* / Q000382:microbiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D010473:Perciformes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014735:Vibrio Infections / Q000378:metabolism / Q000662:veterinary*
D000199:Actins; D017931:DNA Primers; D007334:Insulin-Like Growth Factor I; D005954:Glucosephosphate Dehydrogenase