Inferring the in vivo looping properties of DNA.
The free energy of looping DNA by proteins and protein complexes determines to what extent distal DNA sites can affect each other. We inferred its in vivo value through a combined computational-experimental approach for different lengths of the loop and found that, in addition to the intrinsic periodicity of the DNA double helix, the free energy has an oscillatory component of about half the helical period. Moreover, the oscillations have such an amplitude that the effects of regulatory molecules become strongly dependent on their precise DNA positioning and yet easily tunable by their cooperative interactions. These unexpected results can confer to the physical properties of DNA a more prominent role at shaping the properties of gene regulation than previously thought.
Saiz
Leonor
L
Integrative Biological Modeling Laboratory, Computational Biology Program, Memorial Sloan-Kettering Cancer Center, 307 East 63rd Street, New York, NY 10021, USA.
Rubi
J Miguel
JM
Vilar
Jose M G
JM
D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D007763:Lac Operon / Q000235:genetics; D009690:Nucleic Acid Conformation; D013816:Thermodynamics
D004247:DNA