Pyruvate improves neutrophilic nitric oxide generation in peritoneal dialysis solutions.
To investigate the effects of pyruvate (Pyr)-based peritoneal dialysis solutions (P-PDS) on neutrophilic nitric oxide (NO) generation, we incubated human peripheral neutrophils in dL-lactate (Lac, 40 mM)-based PDS and equimolar P-PDS, and Hanks' balanced salt solution at various pH and high glucose (HG) levels, respectively. The production of NO in various test solutions was determined based on the Griess reaction. Acidic pH, high Lac, and HG induced an acute and substantial inhibition of neutrophilic NO, whereas Pyr in PDS significantly improved the NO generation in both acidic pH and physiological pH, and also in HG conditions. The Pyr protection may be associated with the improvement of glucose metabolic pathways in addition to its alkalization.
Wu
Yi-Tai
YT
Department of Nephrology, Tong Ji Hospital, Tong Ji University School of Medicine, Shanghai, China.
Wu
Zhao-Long
ZL
Jiang
Xiao-Feng
XF
Li
Su
S
Zhou
Fang-Qiang
FQ
D002470:Cell Survival; D002478:Cells, Cultured; D015314:Dialysis Solutions / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D010530:Peritoneal Dialysis; D019289:Pyruvic Acid / Q000494:pharmacology*; D013053:Spectrophotometry
D015314:Dialysis Solutions; D007773:Lactates; D009569:Nitric Oxide; D019289:Pyruvic Acid; D005947:Glucose