title: Opposite roles of the dmd gene in the control of RNase E and RNase LS activities.
abstract: When the dmd gene of bacteriophage T4 is defective, expression of middle genes starts normally but drops abruptly. However, the residual expression of middle genes at late stages continues at a higher rate in cells infected with a dmd mutant than with the wild type. In order to understand the complex effects of the dmd gene, we followed changes in the quantity of mRNA from a middle gene, uvsY. The uvsY mRNA was degraded rapidly by RNase LS at middle stages but stabilized at late stages, suggesting that RNase LS targets middle-gene mRNAs only at middle stages. Furthermore, another RNase targeting middle mRNAs at late stages is also suggested to be inactivated when dmd is mutated. We found that RNase E was involved in the degradation of uvsY mRNA. Judging from the processing of gene-32 mRNA, RNase E activity declines after the beginning of the middle stage when dmd is defective.
authors.affiliation: Department of Biology, Graduate School of Science, Osaka University, Japan.
authors.forename: Takuma
authors.initials: T
authors.lastname: Kanesaki
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hamada
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yonesaki
mesh_terms: D017122:Bacteriophage T4 / Q000201:enzymology / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D004722:Endoribonucleases / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D020871:RNA Stability / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D008565:Membrane Proteins; C092864:UvsY protein, Enterobacteria phage T4; D014764:Viral Proteins; C060035:gp32 protein, Enterobacteria phage T4; D004722:Endoribonucleases; C022537:ribonuclease E