Spin probe study on the interaction of chitosan-derived polymer surfactants with lipid membrane.
Chitosan-derived polymer surfactants, sulfated N-acyl-chitosan (S-Cn-Chitosan), were synthesized and compared with commonly used low-molecular-weight surfactants, sodium dodecyl sulfate (SDS), dodecyltrimethyl ammonium chloride (DTAC), and octaethyleneglycol mono n-dodecyl ether (BL8SY), in their interaction with a lipid membrane using a spin probe method. A suspension of dipalmitoylphosphatidyl-choline (DPPC) spin-labeled strongly (10%) with a spin probe, 1-palmitoyl-2-(12-doxyl) stearoyl-phosphatidylcholine, was mixed with the surfactant solutions. The dissolution time of the DPPC membrane was estimated from the peak height change vs time, which was caused by the decrease in spin-exchange interaction. The times were 2, 4, and 70 s for BL8SY, SDS, and DTAC and 1.2 and 8.8 h for S-C10-Chitosan and S-C14-Chitosan, respectively, showing that the dissolution of the lipid membrane with polymer surfactants was far slower than that with low-molecular-weight surfactants. In addition, the time depended on the length of the alkyl chains of the polymer surfactants. Simulations of the ESR spectra of the DPPC-surfactant systems containing small amounts of surfactants were carried out in order to examine how the membrane structure was changed by the incorporation of surfactant molecules. By this analysis, it was revealed that the rigidity of the membrane was decreased by the addition of low-molecular-weight surfactants in the order of DTAC>SDS>BL8SY, inverse to the order of dissolution times. S-Cn-Chitosan, in contrast, increased the rigidity of the membrane, suggesting that polymer surfactants adhered to the lipid membrane and tightly enfolded the riposome anchoring their alkyl chains.
Nonaka
Ken-ichi
K
Institute for Environmental Sciences, University of Shizuoka, 52-1 Yada, Shizuoka-shi 422-8526, Japan.
Kazama
Shunsuke
S
Goto
Ayako
A
Fukuda
Hiroshi
H
Yoshioka
Hiroe
H
Yoshioka
Hisashi
H
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013113:Spin Labels; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism
D008567:Membranes, Artificial; D011108:Polymers; D013113:Spin Labels; D013501:Surface-Active Agents; D015060:1,2-Dipalmitoylphosphatidylcholine; D048271:Chitosan