The effect of sonication on simulated osteoarthritis. Part II: alleviation of osteoarthritis pathogenesis by 1 MHz ultrasound with simultaneous hyaluronate injection.
In our previous study, we demonstrated the effects of ultrasound (US) on the delivery of hyaluronan (HA) into the synovium, even at molecular sizes as high as 3000 kDA. We hypothesized that a combined therapy with US and HA would have synergistic effects on alleviating the pathogenesis of osteoarthritis (OA). In the present study, we evaluated the effectiveness of sonication on the progress of induced OA in rabbits. We measured the cartilage degradation and inflammation, synovial fluid amount along with protein content and, finally, performed histologic analyses of the integrity of the cartilage and synovium. Low-intensity continuous US at 1 MHz, 400 mW/cm2 was applied to the knees for 10 min bid. Combined treatment with US and HA most strikingly reduced total synovial fluid volume and also significantly alleviated the OA-induced accretion of total protein, proteoglycan and prostaglandin E2 in the synovial fluid. It also attenuated the release of collagen type II and matrix metalloproteinase-3 in the OA-induced joint to normal levels. Histopathology revealed that combined HA and US treatment also reduced the severity of OA-induced structural damages in the cartilage and synovium. The effectiveness of HA with regard to the alleviation of OA pathogenic changes can be greatly enhanced by the simultaneous treatment with HA and US.
Park
So Ra
SR
Department of Physiology, College of Medicine, Inha University, Incheon, Korea.
Park
Sang-Hyug
SH
Jang
Kyung Wha
KW
Cho
Hong Sik
HS
Cui
Ji Hao
JH
An
Hee Jung
HJ
Choi
Min Joo
MJ
Chung
Soo Il
SI
Min
Byoung-Hyun
BH
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000473:pathology; D024043:Collagen Type II / Q000032:analysis; D015232:Dinoprostone / Q000032:analysis; D005260:Female; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000627:therapeutic use; D007270:Injections, Intra-Articular; D019278:Matrix Metalloproteinase 3 / Q000032:analysis; D023421:Models, Animal; D020370:Osteoarthritis, Knee / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D011506:Proteins / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D011817:Rabbits; D013010:Sonication; D013582:Synovial Fluid / Q000737:chemistry; D013583:Synovial Membrane / Q000378:metabolism / Q000473:pathology
D024043:Collagen Type II; D011506:Proteins; D011509:Proteoglycans; D006820:Hyaluronic Acid; D019278:Matrix Metalloproteinase 3; D015232:Dinoprostone