Reactions between human serum gamma-globulin and zinc cations.
Interactions of human serum gamma-globulin with zinc cations in solution were studied by differential spectrophotometry in UV light. Supraphysiological concentrations of zinc caused an increment in optical density of protein solution reflecting the effect of gamma-globulin saturation with the metal. Zinc concentrations below physiological led to hypochromism in the protein absorption spectrum. Conformation changes in gamma-globulin during interactions with zinc are analyzed for the surface and intramolecular binding sites and are compared with the effects of copper cations.
Cheknev
S B
SB
Laboratory of Cell-Cell Interactions, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow. cheknev@riem.ru
Babaeva
E E
EE
Vorobeva
U A
UA
Denisova
E A
EA
D002412:Cations; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry; D013053:Spectrophotometry; D013997:Time Factors; D014466:Ultraviolet Rays; D015032:Zinc / Q000737:chemistry*; D005719:gamma-Globulins / Q000737:chemistry*
D002412:Cations; D011506:Proteins; D005719:gamma-Globulins; D015032:Zinc