Biomaterial coatings by stepwise deposition of silk fibroin.
A completely aqueous, stepwise deposition process with Bombyx mori silk fibroin for the assembly of nanoscale thin film coatings is reported the first time. The focus of this work was to develop an understanding of the control of this deposition process and to characterize the films formed from a physicochemical perspective. The deposition process was monitored by UV spectrophotometry and research quartz crystal microbalance. Both absorbance and film thickness correlated linearly with the number of silk fibroin layers deposited, analogous to multilayered materials fabricated from conventional polyelectrolytes. The polymer adsorption process was stable and reproducible, with control of a single layer thickness ranging from a few to tens of nanometers, determined by the concentrations of silk fibroin, salt concentration in the dipping solution, and method of rinsing. The driving force for the assembly of silk fibroin onto the substrate was primarily hydrophobic interactions, while some electrostatic interactions were also involved. The difference with this approach from traditional polyelectrolyte layer-by-layer techniques is that an intervening drying step is used to control the structure and stability of the self-assembled silk fibroin. The assembled films were stable under physiological conditions and supported human bone marrow stem cell adhesion, growth, and differentiation. This approach offers new options to engineer biomaterial coatings as well as bulk materials with control of both interfacial properties conducive to specific cellular or tissue responses and the potential to entrap and deliver labile molecules or other components due to the all-aqueous process described.
Wang
Xianyan
X
Department of Biomedical Engineering, Bioengineering & Biotechnology Center, Tufts University, Medford, Massachusetts 02155, USA.
Kim
Hyeon Joo
HJ
Xu
Peng
P
Matsumoto
Akira
A
Kaplan
David L
DL
D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005349:Fibroins; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D049329:Nanostructures; D047011:Silk / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology*; D014867:Water
D020099:Coated Materials, Biocompatible; D047011:Silk; D014867:Water; D005349:Fibroins